LOCUS       DAOQEW010000001        71860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_58_length_71860_cov_8.167579, whole genome shotgun sequence.
ACCESSION   DAOQEW010000001 DAOQEW010000000
VERSION     DAOQEW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 71860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71860
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58_length_71860_cov_8.167579"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3299)
                     /locus_tag="PLS35_00005"
     CDS             complement(<1..3299)
                     /locus_tag="PLS35_00005"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNI17052.1"
                     /translation="MNTKLRSRSGKRQQSGVLYEEGHDKAVLNILQAVKKGDFSVRLP
                     AEWDGMAGKIADEINGLIELNAMFSEELKRVSHAVGKKGKLSERASVPEARGEWKTNI
                     EAVNGLIDDLVRPTTEMARVIGAVAKGDLTQSVALEAEGHPLRGEFLSTAKTVNSMVG
                     QLDAFAAEVTRVAREVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMASNLTNQVRNIAE
                     VTIAVANGDLSKKITADVRGEILQLKDTINTMVDQLRSFAAEVTRVAREVGTDGKLGG
                     QAVVPGVAGTWKDLTDSVNSMASNLTNQVRNIADVTVAVANGDLSKKITVDVRGEILQ
                     LKDTINTMVDQLRSFAAEVTRVAREVGTDGKLGGQAQVRDVSGVWKDLTESVNLMAGN
                     LTAQVRNIADVTTAVANGDLSKKITADVRGEILQLKEAINTMVDQLRGFAAEVTRVAR
                     EVGTEGKLGGQAVVPGVAGTWKDLTDSVNSMASNLTNQVRNIAEVTTAVANGDLSKKI
                     TADVRGEILQLKDTINTMVDQLRSFAAEVTRVAREVGTDGKLGGQAQVRDVSGVWKDL
                     TDSVNSMASNLTAQVRNIAEVTTAVANGDLSKKITADVRGEILQLKEAINTMVDQLRS
                     FAAEVTRVALEVGTEGKLGGQAQVRDVSGVWKDLTESVNLMAGNLTDQVRNIAEVTIA
                     VANGDLSKKITVDVRGEILQLKEAINTMVDQLRSFAAEVTRVAREVGTEGKLGGQAAV
                     SGVAGTWKDLTDSVNSMASNLTDQVRNIAYVTIAVANGDLSKKITADVRGEILQLKEA
                     INTMVDQLRSFAAEVTRVAREVGTEGKLGGQAQVRDVSGVWKDLTDSVNLMAGNLTDQ
                     VRNIANVTIAVANGDLSKKITVDVHGEILQLKEAINTMVDQLRSFAAEVTRVAREVGT
                     DGKLGGQAIVPGVAGTWKDLTDSVNSMARNLTAQVRNIAEVTIAVASGDLSKKITVDV
                     RGEILQLKDTTNTMVDQLNAFAAEVTRVAREVGTEGKLGGQAVVPGVAGTWKDLTDSV
                     NAMASNLTVQLRDMSNVATSIANGDLTRQITVDVRGEILQIKDVINRMVAQLSAFASE
                     VTRVAREVGTEG"
     gene            complement(3587..3973)
                     /locus_tag="PLS35_00010"
     CDS             complement(3587..3973)
                     /locus_tag="PLS35_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17053.1"
                     /translation="MINELNRPIQEPNVGRAVLGTSLGQSASYCGATAILSLHQPVIE
                     VSESDGNFNVYADFRPVLENTSMQVAVEFAQQGIILTGGTVERYLPIPTDAYIGHARV
                     TVTGGIARISIPTADAGHRWRSIVMW"
     gene            4181..6157
                     /locus_tag="PLS35_00015"
     CDS             4181..6157
                     /locus_tag="PLS35_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17054.1"
                     /translation="MPSPDETSPSIPQPGFPLDFMLAHLAAIVECSDDAIISKDLNGM
                     ITSWNRGAQHLFGYTACEMIGQPVLRLIPEDRQAEEPRILERLRRGERIDHYETVRKR
                     KDGTLIEISLSVSPIKDREGRIIGASKIARAISELKQGERNLAHLVEALPAAVYTTDA
                     DGRITRYNQAAVDLWGQRPSLETARWSGSWRQYWADGRPMPPEECPLAQSLALNRPVS
                     GLETIVERPDGTRLPVASHPTPLRDASGAPIGGVDILVDLTEQKRAERDMQNSAAELE
                     RRIVERTGELLLSQERLRALASDLTLTEQRERRHLATELHDYLAQLVVASRIRLAQVI
                     PTIHDATASAALTKIDGMLDQALTYTRSLVAELSPHILYQFGLSQSLVWLGEQMKQHG
                     LEVAVKTMAPSFTLPDDQAVLLFQSVRELLFNVIKHAKVDRAAITINVDEDRRELWIC
                     VEDEGAGFDVTDLSVAKNTRCKFGLLSIRERMELLGGECEVSSVPGSGTVAILRLPLA
                     SGALKQPFPLAADTAQTITPSIDASSRNINILLVDDHAMVRQGLRSILDAHDNLTVVG
                     EGADGQDAVVMARSLRPDVVIMDVNLPIIDGIEATRILTQEHPSIMVIGISVRNDPQV
                     HDAMTEAGAVAFLPKESAADQLYEVILRAFPVQA"
     gene            6365..9910
                     /locus_tag="PLS35_00020"
     CDS             6365..9910
                     /locus_tag="PLS35_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17055.1"
                     /translation="MTSVLLTLQRLVPSAAGLAIGFPVFAFLCWAYNPTFLYNLSPSA
                     GTVKPSTMAAICFCGLSLWLQADEAAMTRARRRMAQGFAGLAGLLAAVTMLEYLLSLD
                     LGVDLWLASVPDLAVDSHPGRMAPATAFALFMLGLALIQLDSKHSGQDQASHYLALAG
                     GTIGAIALVGYLYGVQTLYKVGTSNTISIFSAALLLALGLGILSARPTRGFMATLTSD
                     ELGAVMLRRVLPFAIALPILAGWIRIAAQRSGRYDFNFGVALAVSAVMLIILAFLWLL
                     AGNLNRSDGQKKHILQILQQREARHRLALKAGRMGTFHQDLDRHTLVFSPELEHIFGL
                     PAMSFGSTFAAFQDLVHPEDRAHVLKTINYAIQHRSAYDLECRILRQDPPEEAWIAVT
                     GQVLPDSTGHPRQITGVMFDITERKHSVTALQQSEQQLRVITDALPGLIASVDCDERY
                     RFVNAGYEHQFGLSRDQIVGLSVKDLLKEDYSTVQPFIRRVLSGERISFETTLLDERG
                     EQTLLSTYVPDRNSEGMVAGFYALITNITERKRTESALRESEERFRHLFEQASDGIVT
                     ADMNGQYLDVNNRACQMLGYTREELFDLRVPDLLDPHDYPRLRAIKAELIQGHRHSGE
                     WKLRRKDGGTISVEISARLQPDRRWHAIVRDITERKRADAGLRESEAYFRMLADATPV
                     LVWKAGTDRLCSWLNRSWLEYTGRLLDDELGTGRTDSIHPDDRDEFNRVYNQHFSRQE
                     PFELEYRLRRQDGTYGWILDRGVPLLSAAGVFSGYLGAALDITDRKLAEEQLQQWTVE
                     LEKRVDERTQALVHSQARLRALVSDLSATEEQERRRLATELHDYLAQLLVVGRMKLSQ
                     ARPQVRNPKTEQLLSEADDMLTQSLNYTRSLVAELSPQILYQFGLPAALKWLAGQMKS
                     HGLSVSIQCGIDQLTLAEERAVILYQSVRELLFNVAKHAGTGEAMITLDQSPTNQVLI
                     TVADNGRGFNPAILVDTEGHHPGRFGLFNVQERIEAIGGTLSLKSSEGCGTTVTLIVP
                     LKPFPESAVESDLPVDGHVPDVLRTPSQRLRILLVDDHAMVRQGLRSVLESYTDLEVV
                     GEAGDGGTAISIASALKPDVVVMDINMPNIDGIEATRHILSEHPDVVVIGLSVQNERH
                     IEEAMLKAGAAVFVTKERAAGQLYEAIVSTVRTRR"
     gene            complement(9925..10092)
                     /locus_tag="PLS35_00025"
     CDS             complement(9925..10092)
                     /locus_tag="PLS35_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNI17056.1"
                     /translation="MLSWAVTFLVIGLIAGVLGVTGVAGTATHIAYVLFVIFLILAVI
                     GMVMGRRPPAV"
     gene            complement(10379..11443)
                     /locus_tag="PLS35_00030"
     CDS             complement(10379..11443)
                     /locus_tag="PLS35_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNI17057.1"
                     /translation="MALFLLGVMAGFAGMAMAASDEYIAGYAAGLLEHEFHLSGAVVQ
                     VEQGRVAVYAKRLAAEDPAKIVTALKAIPGVVSAEVHTGSAPEGAAGPGVVRATTPTA
                     ESKFLPRGLLVDPFHADLRWPHFGASYHSLSSGGREFASAFGESFSVYRDAAPFQGEW
                     ELGIQAGVFGVFDTEKRSIDLINADYTFGIVASYRADRFSGFIRIRHQSSHLGDEFLL
                     NNPQVVRINLSYEEIDLKMSYDLTTWLRVYGGVGTIVRKDPSTLGRETTQGGVELKSP
                     WLFFGGKVRPVAYADFQTHARTHWTTGQSIMAGLQFENARIGDRKLQVLGEYYSGPSP
                     DGQLFTQRVEWIGMGIHLWF"
     gene            complement(11531..12130)
                     /locus_tag="PLS35_00035"
     CDS             complement(11531..12130)
                     /locus_tag="PLS35_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI17058.1"
                     /translation="MRQGVLSAALVLCASFLIAGGVVVPSPARAADDKGLFEQMDIGL
                     LSIGGRATYIDPKDGSSRWFGGGQVRLHPWRYLAFEGSADYRRNDIGDTRVHTYPVQV
                     SALIYPLGTTRLAPFLLGGGGWYYTTVKGPGGFDDTQNRFGAHAGGGLQFFFNKHVSI
                     DSTYRYIWLEKIESRDQNIVDKKFQDNGHMVTVGVNFHF"
     gene            complement(12175..12516)
                     /locus_tag="PLS35_00040"
     CDS             complement(12175..12516)
                     /locus_tag="PLS35_00040"
                     /inference="COORDINATES: protein motif:HMM:NF017353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNI17059.1"
                     /translation="MRLGHWRLSIVALAGILMMTAAGHAGEQHRLRGTQGTLILAVNQ
                     DQLAGMWKQFMGELKQKWAEFTDDDLMLIEGRIDTLEGKIQERYGDRREEVKRWVDEW
                     FESHGEKRTQS"
     gene            complement(12585..12863)
                     /locus_tag="PLS35_00045"
     CDS             complement(12585..12863)
                     /locus_tag="PLS35_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNI17060.1"
                     /translation="MKKMIAIALLSGVIASPAWALFETNKQLAATATVTLEDAVKHAL
                     KLVPGKAVEAEIGKQDGRTVYEVEIIDSNNKEQKVYVDAQSGQAKIDR"
     gene            complement(12917..13162)
                     /locus_tag="PLS35_00050"
     CDS             complement(12917..13162)
                     /locus_tag="PLS35_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNI17061.1"
                     /translation="MNTDQFKGKWVQFKGEVKKQWGKLTDDDLKQVEGDYDKFLGRIQ
                     ERYGDKKEEVMRWADDWYSRQGQAQQGTQHRETQPPR"
     assembly_gap    13503..13602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13668..13928)
                     /locus_tag="PLS35_00055"
     CDS             complement(13668..13928)
                     /locus_tag="PLS35_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17062.1"
                     /translation="MKVWPGRPYPLGATWDGEGVNFALFSENTTSVELCLFDGPDATR
                     ESHRIRLEERTNQTWHVYLPEGRPGLHYGHCVAACRWQRNCG"
     gene            complement(13986..14525)
                     /locus_tag="PLS35_00060"
     CDS             complement(13986..14525)
                     /locus_tag="PLS35_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNI17063.1"
                     /translation="MKTEKPQASMIKDVQSIRERARQHIEEGALTADYKADRDTVIKL
                     LNEALATEIVCVLRYRRHYFMAQGMNAESVKAEFLAHAGEEQAHADELAERIVQLGGE
                     PNLSPDGLSSRSHSEYVEGEDLEEMIKEDLIAERIAIESYRDMIAFVGSDDPTTRRLL
                     ETILMKEEEHAEDLVSLLK"
     gene            complement(14565..14756)
                     /locus_tag="PLS35_00065"
     CDS             complement(14565..14756)
                     /locus_tag="PLS35_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17064.1"
                     /translation="MMPVSLSGNQFGKYMGLLISIAACALVVGTQARTVTQRTDEQGL
                     MTELGLESAWPHVRTDFSR"
     gene            complement(14875..15363)
                     /locus_tag="PLS35_00070"
     CDS             complement(14875..15363)
                     /locus_tag="PLS35_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI17065.1"
                     /translation="MTSTSSKSNVPRTIAEAGFTRPRRRLYLETRIDEDLGVLPGEVR
                     KVRHVMSSALTVVSPDTSHEEAVSLMAAMNVPVIVVYEGTRLVGMVTDRDVALHRSSR
                     PPTTALTVRDIMSEQVPYCQEDDLLTDAQALMRLSEVDWMPVLDRQGRLAGVLSIYVP
                     PS"
     gene            complement(15393..15689)
                     /locus_tag="PLS35_00075"
     CDS             complement(15393..15689)
                     /locus_tag="PLS35_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17066.1"
                     /translation="MESSEKAASIRQWINPEERVTVDFENEHDLNAEVIECDGQTVTL
                     LLETAFPHYKQHLTLPLSMISVGEDKSHYTRDPDKPVQYGRLRITVHEARPQAV"
     gene            complement(15989..16624)
                     /locus_tag="PLS35_00080"
     CDS             complement(15989..16624)
                     /locus_tag="PLS35_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNI17067.1"
                     /translation="MAVTQPYQAKAFDLFGLSGISDETLRVHFALYEGYVKAANELRE
                     QLSEFIQQGHVDHEQMPAYSELTRRLGFEFNGMVLHEYYFGNLRRGGGAEPPNGSAFH
                     RAVERTFGSYERWKADFCSVGMMRGVGWAICNVDPARGLVSNHWVSLHEHGNVAGFLP
                     ILVMDVWEHAFLLDYQPSERKQYIDSFFSNIGWGVVEDRLQAGLSSVAIPH"
     gene            16919..17575
                     /locus_tag="PLS35_00085"
     CDS             16919..17575
                     /locus_tag="PLS35_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17068.1"
                     /translation="MARARIVIADDHSLVLEAYRQLLEPDFDVVGTASNGQELLNIAP
                     ALNPDIILLDISMPTMNGLEVTTQLRAAIPHAKIIFVTMMSEPFYISQAFQMGASAYV
                     LKQSASTELLSALNAALKNQRYISPQLSLEVQDAIETPWVKPEGFSSKLTPRQQEVLQ
                     LLTKGMSTKEIATTMHVSTKAVEFHKGNITRRLGIRTTAELTRFALSQGLTKLSDQHP
                     "
     gene            complement(17585..18268)
                     /locus_tag="PLS35_00090"
     CDS             complement(17585..18268)
                     /locus_tag="PLS35_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17069.1"
                     /translation="MKPRVLIADDHTLVAEGVEKLLEHDFQLCGRAADGRALVRAVEK
                     EKPDIALVDIALPLLNGLDACRQIKKSVPEVKLLVLTMHSEQYFVTEAFRAGVSGYVL
                     KQSVADELVFAIKEVLKGRLYVSPSVAENLVAQALHPVAPAPAGAPPSNDSLSGRQRE
                     VLQLIAEGQSTKEIASTLNVSIKTVEFHKTRIMKQLGIHSTAELTKHAIAIGLIAMPQ
                     QPTVPGSQP"
     gene            complement(18357..19649)
                     /locus_tag="PLS35_00095"
     CDS             complement(18357..19649)
                     /locus_tag="PLS35_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17070.1"
                     /translation="MSCSIVCLFSVGAIGDDLFGLGQDFALAEEPTPIAGEPFRTTLF
                     GEEIYVPPRDRRSVTAISFGIQAIPNGPTQQEVLPFGALYVWRNWDDDNRRLRGTFSG
                     VVNDVDYTIGLRDYPNWSLLFTWDNFILPFGRSEYVQGQRQRGTELEWSYAFAGAGLG
                     YRLPVHPFRQDSAVNISLTYEAGYRWFKGTGHTSADYGVPKDTYEGRIHFRIRADALK
                     RNLMELPHEGFTLGGDVVHGHRAKWDQWGGAPFNTQDFKKEQTYIQASAYALLATGLP
                     FIESDKHRLLSSIHAGIGKDLDRFSAFRLPGRPTGYEWEAISLPMIHGVAFNELFPTR
                     YAVANLQYRYEALFFLYPYVEATWGLVERPVFTSNTAVKSITDSMPALGGGLVTGAPW
                     KSQVELNYTYNFGIYRDPGGAPPTKGGHGIIFFWSKEL"
     gene            19930..20793
                     /locus_tag="PLS35_00100"
     CDS             19930..20793
                     /locus_tag="PLS35_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17071.1"
                     /translation="MERTYRTQPHCFNGTSAHVPRWRCCLAAAGLFLLTSTTLFANPI
                     EVIYPEGVSEGFVTLKTLDGKKLADGELSQVTTGADRLASRLTFRFADGSLYDETVTF
                     SQKKHLTMLSYQLTQRGPAFPEPLVISLNGETGQYQVRQREKTPDEKLITGRLDLPKD
                     VYNGLTITVLKNLRGQTGAIVHMLVFNPEPKLYEVELARVDEEDTRNTTRKETKATAV
                     HYSLKPRLGWFTAALARLLGRTPPDYHFWLIKKDAPAFVRFEGSLYAGGPSWRIEQVS
                     PRVKQARVPEP"
     gene            complement(20801..>21496)
                     /locus_tag="PLS35_00105"
     CDS             complement(20801..>21496)
                     /locus_tag="PLS35_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17072.1"
                     /translation="SNGRAIVMIGIMLGGIVCGEAGPFLVWSAEPVLSVAAAQHDQVH
                     GALAAEGTVDRFILDPRGEVEGLFLADGTHLYVTSRAADQLVRAFAPGNAVRVYGRLM
                     PDERLVQADAVINVTTGTTFTVPLRLDLPMQEQEHTLSMTEMSAAGTIRAFFYHPLKH
                     TIQGMLLSDETQVRFPPDASPALRRSFHVGEHVRVRGNGTINRFGRAVEALAIGRNSA
                     ALVPLDASLQRLP"
     assembly_gap    21497..21596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21597..23107)
                     /locus_tag="PLS35_00110"
     CDS             complement(<21597..23107)
                     /locus_tag="PLS35_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNI17073.1"
                     /translation="MNATAKIEDYALIGDTHTAALITRQGSLDWLCLPRFDSPACFAA
                     LLGTPEHGRWLITPHEDNTTIRRRYRGDTLVLETLFETPTGTAMLIDFMPPRDRDINV
                     VRIVVGVKGTVRMKMDLTLRFDYGSIVPWVSHQRRFLKAVAGPDAVYLETELELQGVG
                     FSTVADFTVTEGQEIPFTLTWRASHDSPPRPLITERALSQTELWWKKWSQRCTYQGSW
                     RNHVVRSLITLKALTYAPTGGIVAAPTTSLPELLGGVRNWDYRYCWIRDATFTLYALL
                     SAGYHSEAKAWREWLLRAVAGKPSDLQIMYGIRGERRLTELELNWLPGYEQSAPVRIG
                     NAASQQFQLDVYGEMMDTLHQARELGLAASEDAWRVQHAIMEFLETAWDRPDEGIWEV
                     RGPRRHFTHSKVMAWVAVDRAVKAIERCHVDGPLERWRALRETIHEQVCREGYDSNRG
                     TFVQYYGGTSLDGSLLMIPLVGFLPAFDPRVQGTLHAIERELTVDGLVMRYQPT"
     gene            23207..23533
                     /locus_tag="PLS35_00115"
     CDS             23207..23533
                     /locus_tag="PLS35_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17074.1"
                     /translation="MRKVLAKKTQPRKKTSSAKKRTVKRDIEPAAHVIERKTKKKPAT
                     SAKAKKTTLTKKKTSKTTRKRSPGSSRNAKSVGRAVGKVIGRAIGTVERALTLVRPNS
                     NTPKKQ"
     gene            23655..23978
                     /locus_tag="PLS35_00120"
     CDS             23655..23978
                     /locus_tag="PLS35_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17075.1"
                     /translation="MAFQVVSTLVSTTLLMALALPAALTAANPAPESGQPQPPSTQQT
                     PPTAPPSRIDPGIQKAPDTVPNPKSIVPPPVVDPKMAIDPERPGGPAHPKPPSPADKP
                     PMPPR"
     gene            complement(24036..25307)
                     /locus_tag="PLS35_00125"
     CDS             complement(24036..25307)
                     /locus_tag="PLS35_00125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNI17076.1"
                     /translation="MNQQSRNPVVIVGGGFGGLAAARALHHLKGEVILIDRMNHHVFQ
                     PLLYQVATAALSPADIAWPLRTLLRAQQNVRIMMDEVRAVDRSARRIELRNSEPVSYS
                     SLILSPGARHSYFGHNTWEQDAPGLKTLPDALEIRERMLMAFEKAERLKGTPEARDEL
                     TFAIVGGGPTGVELAGALAEIGRKAMTPDFPVLHQAASRILLIEGGPRILEAFPPALS
                     SRAQASLESLGVTVLLNCRVHDVTSKGVMAGSEFISSAHVIWAAGNVASPILASLDVP
                     LDSSGRVIVGPDLSLKEDPWVFVIGDAAHCLSAQQKPLPGLAAVAMQEGRYVAGIIAR
                     CIPDGQRSAFRYRDRGILATIGRAKAVAQFGPISLSGVVAWLAWCFVHIFFLIGFRNR
                     ARVMFEWIWYYVTFKPGARLIYWKDRRINPQ"
     gene            25565..26653
                     /locus_tag="PLS35_00130"
     CDS             25565..26653
                     /locus_tag="PLS35_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI17077.1"
                     /translation="MKIAQVAPLWESVPPVLYGGTERIVSFITEELVRQGHEVTLFAS
                     GDSTTRARLMPMCGEALRPSKVVMNYEAPLTAMIEKVFGSAPEYDLIHSHLEFIPFPL
                     AKRCRTPMLTTLHARLDQPELIPVFRKFADLPLVSVSEAQREPLPWANWQATIHPGIP
                     HSLYTPQYNQGQYLVFLGRVAPEKGLDRAIEIAKRVEMPIRIGAKIDAQHAEYYKSVI
                     EPLLDHPLVEFVGEVTDLEKDDLLGDAYALLAPYAWPEPFGLAIIEALACGTPVIGSR
                     RGAIPEIIEHGVTGFVCDTLDEMTQAIRHVSLLERTRCRQAFETRFTAERMAQEYVAV
                     YGQILGLPDKAPQPSSDLASGLPAAGQI"
     gene            complement(26726..27439)
                     /locus_tag="PLS35_00135"
     CDS             complement(26726..27439)
                     /locus_tag="PLS35_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNI17078.1"
                     /translation="MNRRMAMYAGTIALALMTGTPAFSQVSTGSGAPPPDSTMGKGAG
                     TSTPKESPSFDKKTDLTGGKQGIPEEYADTPVRQGKLVEMKDSKYLNASVYGTNGEEI
                     GKIQQVMKDEKTGDIEYVVFVSNESKRPQPIRWSQFKTKGDKLQLTLKKEEIQNALPM
                     SSSKDKSPDLQEYKDKMGQVREAPTVPGSAGVPGQKGPDATGSMGEESVGGGGPSGTS
                     GLPSGKAPGFEGGNPSSKR"
     gene            complement(27493..27855)
                     /locus_tag="PLS35_00140"
     CDS             complement(27493..27855)
                     /locus_tag="PLS35_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNI17079.1"
                     /translation="MMATHIGRRLLTASTIEGTAVQNAAGEDLGEIHSLMIDLEKGRI
                     AYAVLSFGGFLGLGDKLFALPWGALTISPGGDFFILNVPRERLEQAEGFDKDRWPDMA
                     DTAWAERLHTYYGYKPYW"
     assembly_gap    27936..28035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28036..30347)
                     /gene="treY"
                     /locus_tag="PLS35_00145"
     CDS             complement(<28036..30347)
                     /gene="treY"
                     /locus_tag="PLS35_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HNI17080.1"
                     /translation="MPRIPIATYRLQLNHTFTFRDAGRIVPYLYALGITDCYVSSILK
                     AVPGSMHGYDLVDPECLNPELGTEEDFAAFTAALKQYDMGLLLDVVPNHMGIRSTENR
                     WWWDVLEHGPGSRYATAFDIDWSPLKRELEDKVLLPILGEQYGAVLENQDIRLVYENG
                     GFIVTYFQHRFPVAPQSWEGILSFRLEELTARVGEQDEHVQELLSILTALRNLPARKE
                     RDPKRIAERYREAHMIRRRLAALLQESETIRVHLSATVDTYNGVKGDSASFDRLDALL
                     DEQSYRLASWRVASEEINYRRFFDINELAAIRTEETRVFQESHQLVFRLLKSGVATGL
                     RIDHVDGLYDPEHYLVQLQEWAARELEPAASGEPASLFVVVEKILGKGESLPVTWPVS
                     GTTGYDFLNLVNGLFVQSAKERSMDALYQRFVGQRIVYDDLVYLTKKLIMRASMSSEL
                     NVLGHQLNLLSEKDRQYRDFTLNSLTHAITELIACFPVYRSYITADQKDVLERDRTYI
                     MMAVNRAKRRNPTLNSQVFDFVRDLLLKRLDDRIKLAGPDQVRFVTKFQQTTSPVTAK
                     GIEDTAFYIYNRLVSLNEVGGEPAHYGLSVDAFHKALRERRAHWPHALLATSTHDTKR
                     GEDVRSRINVLSEIPERWRAALASWAKHNRRFRVKVDETVAPDRNEEYLLYQTLLGAW
                     PSTPMDQPQYEEFKKRIEQYMIKAIREAKVHTGWINPREDYEEGVKRFVQAILDRSVS
                     NPFLEEFLPFQAFVARCGMNNSLAQLLVKIT"
     gene            complement(30372..31646)
                     /gene="glgC"
                     /locus_tag="PLS35_00150"
     CDS             complement(30372..31646)
                     /gene="glgC"
                     /locus_tag="PLS35_00150"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNI17081.1"
                     /translation="MEKHGRISQPRVLAMILAGGKGTRLMPLTEARSKPAVPFGGAYR
                     IIDFVLSNFYNSGILGMHVMVQYRSQSLIEHLRKAWRIGDGERQFVTVVPPQMNSGAG
                     WYEGTADAVFQNLNLIRDFAPDIVAVFGADHIYRMDIRQMVQFHLDREADVSVATLPV
                     PVHAAQGFGIVEIDRTSLVVGFDEKPPVPKSMPGDPAHALSSMGNYLFNADVLIQALE
                     EDAKQEGGSHDFGRDILPRRLKTDRVMAYNFLDNEVPGVGYHEEPAYWRDVGTIQAYW
                     QANMDLLGDTPPCDLGNREWPIRTEPSYGPPASLVNCYVDHALIGEGTRAIEADIRRS
                     IIGRHVRIESGAYIEDSIIFDHTHIGAGAKLQRVVVDRHNTIPANVDLGPRGQERGEE
                     MDAPSEWAESGVLVIPKPQAATEEQKRRLPSY"
     gene            complement(31869..32432)
                     /locus_tag="PLS35_00155"
     CDS             complement(31869..32432)
                     /locus_tag="PLS35_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNI17082.1"
                     /translation="MIAIGREISPSTVQAYHQGEIRTLVLEELRGKWLVLMFYPGDFT
                     FVCPTELAELARLYPEFVTLGAEVCGVSTDSVYVHKAWHDSSPMVRTVEFPLIADSTG
                     ALSREFGVYLEEEGVALRGTFLIDPDGVLRAYEVHDNSIGRYIGETLRKLQAAIRVRE
                     GEGEACPANWQPGQPTLTPGLDLVGKL"
     gene            complement(32479..33933)
                     /locus_tag="PLS35_00160"
     CDS             complement(32479..33933)
                     /locus_tag="PLS35_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17083.1"
                     /translation="MRRKTTRTAGKAVSHLVSVGVVLAMSVAYVYADRDGKELRHTVN
                     PAIEKTKEKGFEGVDFSYDLFGGPPGQSSARLAQEAMKKDQEEKPKVMAQQARLLQDR
                     YRLECKTHSTVRMTKGKPQPIGPTVQLKNGLTWESLSQMEPEEIRKQKAFPAGFMRLP
                     HVKHEVGGMVFPEEQIHQFPRLERFDVDLDLPACFLPEFPPPIFLTTHPELGDVSQGE
                     VLSVENFDRLFRGLITPVQLDGLRMLVTQFPQEEFNLTADRKSPKPSAGVACLDCHVN
                     FHTTGQFHLNPDTRPQRDRLRLDTVSLRGMFNQQIHGSKRSLRSVEDFTEFEQRTAYF
                     NGDHIRAAKKGISIIDRLQVAHMAQIQNMIDFPPAPKLDPMTGRLDRSRADRQEIAGE
                     DLFFGKARCAVCHQPPAYTDHMMHDLFLERFGAEADGPIKTFALRGIKDSPPYFHDGR
                     LLTLEDSVEFFNVVAGLKLNNDEKAALVAFLRAL"
     gene            34165..34386
                     /locus_tag="PLS35_00165"
     CDS             34165..34386
                     /locus_tag="PLS35_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17084.1"
                     /translation="MNCHRCSNLMFPVALQDWGGGLMNQDVAAWRCFACGEIVDQLIA
                     QNRTRGGEDAEEWRRGGARRRVNGIGLLR"
     gene            complement(34481..34681)
                     /locus_tag="PLS35_00170"
     CDS             complement(34481..34681)
                     /locus_tag="PLS35_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17085.1"
                     /translation="MTTLLVAVVLIGMILTGLYAFGTFSTPYTEAFRFGFYLLIALAL
                     VAVVLVVGRQPFEGYDPTPNSP"
     gene            35159..36001
                     /locus_tag="PLS35_00175"
     CDS             35159..36001
                     /locus_tag="PLS35_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17086.1"
                     /translation="MTVHMNRFLPHQGLHLATAVGISMMLSGVPVAMTSAHAAEETKT
                     GDTSQLREDAQTLLKGNRTVLATVLAVTSDQIKVDIGEVQPRFLPLKQAQQKGFPKVS
                     EGDDLIVVLNEQNLLVDYHPLDGGTSAHTIIRGELSQTLPTGHETAVIKSGGKEQSFK
                     IRSQARSKVAAIPRGATAVFLIDETNQIADVSLSRQQAAQLAGAHSQPIKGQHQQVEG
                     TVTSPLQSNHITIRTPGGTDKPYEVRDILQSKIATLHKGDAVVLLIDGDNKVIDVAIP
                     PKGK"
     gene            complement(36034..37401)
                     /locus_tag="PLS35_00180"
     CDS             complement(36034..37401)
                     /locus_tag="PLS35_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI17087.1"
                     /translation="MTQARILIVDSNVGAATDLRDMLSVDGYEVSAVRDPKDAGGYLE
                     RIPVDLILTDLPPETEELTCLRQLRGLCPTTPIVLMTAHGSLRTAVEGLKAGAFDYMS
                     KPFTAEEVRSVARRAVEHTHMRAEQAASGEAAPDDTSPFASLVGSSPTMVQVYKAIAR
                     VAQTDSSVLLQGESGTGKELIARAIHASGPRSSGPFVTVDGGALADSLLESELFGHER
                     GSFTGAISSRKGLLEQAHSGTCFLDEVADLSPALQGKLLRVLQEKEIRRVGSTATTAL
                     DVRIIAASKLPLEPLVRAGTFREDLYYRMNVVTIDIPPLRERLDDLPLLTKRFLRLYG
                     SMKMPPVTELSAEALALLRRYWWPGNVRELEHAIERAVTLSPYSVICPEDLPPAVKDA
                     QPEGLARAQGWVTLEDLERNHILQVLDSHARDLGRSATILGIHRKTLLRKLRHYGLAA
                     GYGVA"
     gene            37951..38556
                     /locus_tag="PLS35_00185"
     CDS             37951..38556
                     /locus_tag="PLS35_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17088.1"
                     /translation="MKRWLACCASVIVTAIWFPCSSGALEFTADRVTHTDRGFHRARI
                     FYHDGMWRLEHNERGPVSITIVRLDRNQVWHLLPVTHHYKTVSYDRDYALHLATTLDH
                     EISRETIGVQILDGHPTVLYEVLTITPGGGRESYYQWIATDINFPLKLAKKNGDWMVE
                     YRHVKFGPISDVFFELPRHYAPMDDDQHTASSPEPVRRMSP"
     gene            38553..39110
                     /locus_tag="PLS35_00190"
     CDS             38553..39110
                     /locus_tag="PLS35_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17089.1"
                     /translation="MTTRTSRHQMTGARDHIVIGLLPNMGAVREALEALRARGISDDQ
                     IGLAMRQAEERLSDEESPSPTAQDATTGMVGGGIIGGLAGLLTTTGVVAIPGLAPLLA
                     GGSLIGLLGATGASIVAASGLGAVAGGLVGALISINVPETAAKHYADAVHEGQILISV
                     KTDGDPAQVEELLASLGATTDRQSP"
     gene            39237..40106
                     /locus_tag="PLS35_00195"
     CDS             39237..40106
                     /locus_tag="PLS35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNI17090.1"
                     /translation="MAKQQASRTKSNTPAAQKVVSQNGSAEPPVPHHEPIKTLVVDIG
                     GTGIKSMIVNEVGEPIGERLRLPTPKRKTPPSVLKTIHRLAKLSGRFHRVSVGFPGTI
                     RNGVILDAVNLDPSWKHFNLIAALGKTLRKKPVRAANDADVQGYGAISGKGVELVLTL
                     GTGVGSSLFIDGRLVPNLEVGKNRLRGSELERIGKKLWNNRLLKVIAKLEKMFHYDRL
                     YIGGGNAVQVDIGRVPGNVTVVSNLNGLLGGIALWEEAAHFAASRQELANNNANHSAP
                     PKPAAERAAPRGR"
     gene            40491..41171
                     /locus_tag="PLS35_00200"
     CDS             40491..41171
                     /locus_tag="PLS35_00200"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNI17091.1"
                     /translation="MGDKERLISGLVGGGLLLHSLRGPFSGLSALKALLGLALVQRSV
                     TGHCAGYHMLGMNTHDQTDTSSIGRSKVHSDRAIKIERSITIQRSAKELYAFWRQLDN
                     LPTIMSQVRTVEPLDDRRSHWVINTLPGAPTVEWDAEIINEVENERIGWKTLDGATVE
                     HAGSVRFNPLNDERQTRVTVTLQYDPPAGAIGAAIADLFGQEPGQKIARDLERFKEAM
                     ESHTTVSQ"
     gene            41241..41561
                     /locus_tag="PLS35_00205"
     CDS             41241..41561
                     /locus_tag="PLS35_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17092.1"
                     /translation="MNMWRGLTVAVMVMGATVISGVPGGAAQKESMEKTPKVEDDTSD
                     KSAGKASKDKVIKKRRSSGKSNAEDTTDAKSQPKSQESREGTPGTTLMFRSDGAGNSG
                     AAGK"
     gene            41612..42739
                     /locus_tag="PLS35_00210"
     CDS             41612..42739
                     /locus_tag="PLS35_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17093.1"
                     /translation="MPKQNRHIRLFAAMLGLGIMSATGVYADQAREQREAGLQGQSDG
                     RVHNADITGGFYDDRYKLDDWYYDFYDSPGYSRADSTSVIQASNENTATMADAAHPSL
                     RHHRPYRTDQNVPATPYAGDPWFYAQRDPMYGIPETSSDAYGGNVIKGTIKAAKQVRN
                     RTTGDQNTVVLLTTPDRRQVTADLGSSRRTMDLALTKGDSLVVAGPWEDVGPYSVLMA
                     QQIRTEAKRVTLNRPIGAARGDRTVEGRIQQFRDIRMNRSGELHRAAAVQTPDGRMAL
                     VDLGPDNQDRTPLRAAAGDRMVAQGPVVQVGNYPVLWANRVAINDGMPVDVTRTTAPT
                     DFVPATGNRQENCLGAGCDNQPLNQGQPGNRNSNAMDGTTR"
     gene            complement(42957..43307)
                     /locus_tag="PLS35_00215"
     CDS             complement(42957..43307)
                     /locus_tag="PLS35_00215"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HNI17094.1"
                     /translation="MAQRSTNKADLEYALMMTILNFHTEFMRSNYANVQVHLSQDEIE
                     VTLTRQGSVPAERRLAESPEGRSLLRQVHEAMFLSCERILRERIEPVVERKVREMVTS
                     LDPVSGRSSITIRL"
     gene            complement(43528..43887)
                     /locus_tag="PLS35_00220"
     CDS             complement(43528..43887)
                     /locus_tag="PLS35_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HNI17095.1"
                     /translation="MATTFSEAAKATRKKKVIKLVGVDMYIVTEEGIPKFPDGEFGPF
                     KCEFISNRGTKVWPGFVSPDLMMVNWYRCRFMATGDVTDADVDTFLAVLTKKGWWWSA
                     AQKLWTHDGEPAFSKAY"
     gene            complement(43923..44936)
                     /locus_tag="PLS35_00225"
     CDS             complement(43923..44936)
                     /locus_tag="PLS35_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNI17096.1"
                     /translation="MAQHVVTMLPGEGTGPEICEAVRLVIDASGVDIKWEYEEIGLGC
                     LEKYGTLLPDKTIKNIAKNKVALKGPTTTPVGTGHKSANVTLRKVFDLFANVRPAKLI
                     PVLKRPWDKIDIINFRENTEDSYAAIEHMVSDEVAQCLKVITWPGSYRIADFAFQWAR
                     ANGRKKVYCVHKANIMKMTDGLFLDAFREAAKKYPEIETGDVIVDNCCMQLVRNPAQF
                     DCLVLPNLYGDILTDLCAGLVGGLGFAPGANIGDNCSIFEAVHGSAPKYAGMKKVNPS
                     AVLVSGIMMLRWLKETAAADRIEKAMFAVLHEGKHVTYDVGGTATTDEYAQAIVDKMQ
                     GKA"
     gene            complement(45188..46579)
                     /locus_tag="PLS35_00230"
     CDS             complement(45188..46579)
                     /locus_tag="PLS35_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNI17097.1"
                     /translation="MRGRRTKTCPPSWTVPFSSYAEVTEFLRGVEVDCILRGQPSDGT
                     VSLGAVARCLHELKRPDLAYRSIHVTGTNGKTTVSRMIGALIRATGLTVGVYTSPHVT
                     NFRERIAINGEPIGEDELVDACNHVKAFLDVHGLQLAPFEFLTAAAFFAFRAAKVDYA
                     VIEVGIGGRQDATNVIAPELAVITTVEYDHADMLGDTLEQIAGQKAGIIKPFTPVLCG
                     PMPEGPRAVVLSRAAALKAPVLLSGQDYEVTEFIRDGYAGLCTVRVGASTHPQLRLRS
                     PAAFMAANAAHALMAYDVLRKRGLLPDIDPDEIGRVFEKTEWPACCEVLSGTPAVLLD
                     GAHNAPAAMRLATILRTTFEGRRSVLLVSLARDAQWEQVLRALAGAGADRLIVTGGPQ
                     DRAVPPDAIAEQWRTCSSVAAEVVDSTEAAFRRAVQAAGPHGVVAVTGSPQLAGYCRP
                     LAKTFPAQALVQP"
     gene            complement(46618..49899)
                     /locus_tag="PLS35_00235"
     CDS             complement(46618..49899)
                     /locus_tag="PLS35_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNI17098.1"
                     /translation="MHTTNGSDQRHRDAQRMELRSYVSLAGETIAKVWPMTSVIARNP
                     LQGFEHLRFDEAVERAEQLFGGSGTLSGEAFREAFRRGRIQEKHFEEALRGQAGDRQI
                     TFGDRQVTHLDVLRAIMVAVGRDHDRVGTAATAPPTPTGDSVTVPHLAAWLGRMLPRE
                     TLETERAAGLQYIPGEWPCESTMADWCDRTLGVTITDEINRQMIKWCAAFCDEGEAAW
                     PMPKREETFFRAWKAAARYDFSLRCAGVERAASKIAALADRPEDVLLESLEMLHIPHA
                     IWQSYLSRHLAALPGWAGFIKWREDQDAHVWQSVYRIDLVKYLAVRLFYERELVATAC
                     RASLGCEGTASAVLEYGKRYPYTLWFRRASMSGDLPSALLPEASRLQRAGRSYDPAAW
                     EQDCRRWFEEQPAGERTYRVEAQNLVSLSGALAVPVHEITSTSPGDLAMLLRWMREFT
                     PAKQRVAWLEAFERSHQCEVLRKLELPAGEDARIAAPGAMTARPVAQFVFCIDVRSEV
                     FRRHLENRGGYETYGFAGFFGVPFSYRSLDEQRELPLCPVLLKPKHLIREVPRTYQDA
                     MAERRKHSSHLVKAGHELLHDLKHNVVTPYVMVEALGWFFLLPFLGKTLCPRWYHRFA
                     AWVKSWVVPPVATTLTVDKFSTADAENMLAAEQRLQIAWWLRKHGQRAGSLVTGAVLD
                     EVRRQALEEDEESASHPGALGRLLGVCSTEETDALVTLRRECRLTYRGTRSRLDRITR
                     TGFTVNEQAYYVETSLRLMGLTGSFARLVVLCGHGSTSQNNPYESSLDCGACGGGQGL
                     PNARAFAMMANRPQVREILASRGLTIPPDTQFVAALHDTTTDRVQVADLEDVPSTHRK
                     ELAQILDDVHEAGGEAAAERWVALTAAESHEGTRAARQELERRSVDWAQVRPEWGLSR
                     NSVFIVGSRRLTREADLNGRSFLHSYDHAMDGDGKLLEIIMTAPLIVAQWINMEYYFS
                     AVSPEVFGSGSKIYHNVTGGIGVMTGSQSDLRMGLPTQTVMCGEAPYHEPMRLTAIIE
                     APRERLSAIIERQPLLEKLFNNRWLLLIACEPAEAKYYRYDGGKWFVVADMAEQVGEA
                     PVPQA"
     gene            complement(50148..50945)
                     /gene="cysD"
                     /locus_tag="PLS35_00240"
     CDS             complement(50148..50945)
                     /gene="cysD"
                     /locus_tag="PLS35_00240"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNI17099.1"
                     /translation="MRHLRELEDQSVYILREAYKHFDGLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLVHIDTGYEMPELIEYRDRICRDWRLNLIVGQNKAALADGMGPDRGRVV
                     CCTAMKIDALNQTIAQHGWSAIILGIRADEEGTRAKERYFSLRGKEGEWNFRDQPPEL
                     WEQFQTKFPPGSHIRVHPLLDWTELNIWEYLEQERIELPSPYFDRGAGTRYRSLGCVP
                     CTGTVDSCASTIPQIITELRRTTTAERAGRAQDEGRGMETLRKLGHM"
     gene            complement(51283..52695)
                     /locus_tag="PLS35_00245"
     CDS             complement(51283..52695)
                     /locus_tag="PLS35_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI17100.1"
                     /translation="MAWHLLLLEDEASVREAFALRLRDKGYVVQTAETGEEALGLLRS
                     FEPDVLIVDLVMPNLSGLDVLARVKQMSPALPVIVLTARGTVKDAVEAMRLGAFDFVA
                     KSIDMDDLLHTLRRVTDFLTLQRQVQLHSGQDAARYALDRVIAKSPVTKSFLAQVQEL
                     VRNDRVTVLLQGETGTGKQYMGRVIHYNSARGSKPCVEVDCPSIPRDLFESELFGHEK
                     GAFTGAAGRKCGLIEMAEGGTVFFDEIGDLPLALQAKLLRVIEERSLRRVGGMNTVPV
                     DVRFMAATNRDLKAAVAKGEFREDLYFRLNVVMLTVPPLRARPEDIIPLAEHCLLRSA
                     LALKKPVRELTESARQLLQAYHFPGNVRELGNLIERAVIFCNGPSLDAAHFPADLAAA
                     VAPASLPGLPGAVQPTAAPAEADPLMVSLSFKVGERSLSDLEDAIIQAVLERSDGNKT
                     LAAKHLGITRWMLDRRRKAY"
     gene            complement(52776..54749)
                     /locus_tag="PLS35_00250"
     CDS             complement(52776..54749)
                     /locus_tag="PLS35_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17101.1"
                     /translation="MASSRRSPTTRSRPVQSEIAAAEVLAWLSDCIQGGLSFVCKGIL
                     IFENSQFAELVESGLPRVEGHEHALRKRLLDDAIAWNNRPLGERKSVEYQLEGRDGGR
                     LYYVCRFNVVPYRGERGVLMVLEDVTERVMLAQDASHVARFQSVLARIGTLAVSGVSA
                     QELMNEAVKETAAALEVELCKILVPRAHDGQLYIMAGSGLQSELIGKLTIEGGTHSQA
                     GYAIAKRLPVVVDDLRRETRFSPSRLLTEHGAVSGMCVPMLVEDRVYGVMTAHSKRVR
                     KFSQQEQEFLCTMANTIGTVLERRWHEETQMDFYHRLFLSAQDGVMMTDTTGRILEWN
                     PALERMTGWTREEALGQRPAILKSGKHPPEFYDRLWASIRAGQPFVERFVNRRKDGSE
                     FLVWESVNPVKASDGTTQYFMAILTDLSEREQMLEALRHTEQVKLVGQLAGGILHEVR
                     NPLIGLGSLATHLADQASLPQEARDRCRLIAREAARIDELLESHLGQLRPRPFDITSC
                     DLASVLDDMLTLLRPNIVKNQILIKTSVAPDLPSVDASKAHILQVCLNIVLNAIDAMP
                     GGGHLHVTLVPEARRVPGVLMRFADTGKGIPADDLERIYEPFFTKGKAKGVGLGLTIT
                     RDMVERHHGQLVIQSPPGRGAVVDVWLPLKHEA"
     gene            complement(54862..55185)
                     /locus_tag="PLS35_00255"
     CDS             complement(54862..55185)
                     /locus_tag="PLS35_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNI17102.1"
                     /translation="MLTLHPMKEIRIVVEGEHLKIVTSLLDRVGATGYTIINNVSGKG
                     HHGFHEGHLLFNDTSSQVIVFTVVPEERIEPILAGLGPLFHKHSGAMFVSDVAVTRRD
                     HFVTP"
     gene            complement(55203..58559)
                     /locus_tag="PLS35_00260"
     CDS             complement(55203..58559)
                     /locus_tag="PLS35_00260"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNI17103.1"
                     /translation="MAIETRAFTEAQRMELRSHVELAGEAIAQYWPMRTFIHHNPLHG
                     MEDMPFPQAIKRGEHLLGGKGYLSNAIYRRFFEQGRIGLNDVSCVLAPLASDRCVTFA
                     GRELTHLELMRVSMVHGLVDLPAKLQDSGAAGDTALDDLLAWLGTAVAGTRIRQPEPL
                     VPWESLDLLSHETLSAWCDQTLGTCIVSDINEQMIKWCSVFLDEGEASWVMPNREQTF
                     YRAWKRLARYDAGLGLLGIQDAATKIDALGERPEEAVLSCLVDLGIPKSAWEAYFALH
                     LSALPGWAGYIKWRAEEQHHPWQVRYPIDLVKYLAVRLFYEREFVEYHCRRALNRPGQ
                     YDAIRAILDQAPYAHWLRRQLTARTIPPWAIREAEEWRRSHQAATLDEWNQVGSRLYE
                     QTSEWRGTEAMKRDARRLLALAAALGTDPALISQTSVADAATVLNWLDGFPAGQQGQR
                     WLEALEAKHRRDVVDELRAVTKQLRATDDRAAPAGSARPLAQMVFCIDVRSEVFRRHL
                     EQLGGYETFGVAGFFGIPVKYQAFGEEHPVTHAPVLLKPKNHIREIPRSYHGAAASRH
                     RLFAQLSHVAHTLLHDLKENVITPYVMVEALGWFFSLPFFGKTLCPLAYQRLTSSLKS
                     LWVPPLATTLTIDKLTREEAEEMVAVEQRAVIRAALRQEFPALGSAITPALIDAIRVG
                     VMEDAEGQRGGDVAHILGISPVEVAALYERFRRDFNLTPRGMSSRLQRITQHGFSVSE
                     QAYGVEAALRLMGFTSGFSRLVVICSHGSTSDNNPYESALDCGACGGNSGLPNARAFA
                     SMANNAAVRKVLETRGIRIPGDTHFLAGLHDTTRNDVRIVDMEDIPATHRKDLVRLLE
                     DLREAGAQAAQERGLALGGPVATWSRKDPHTKAARRSVDWAQVRPEWGLSRNNLLIVG
                     RRELTRPLNLQGRAFLHSYDHRQDSSGKLLEAIMTAPLIVAQWINMEHYFSTVDNEVY
                     GSGSKVYHNIVGRVGVMSGANSDLRLGLPAQTVLDGSMPYHEPMRLLTIIEAPRTRVE
                     SVIATHPGLERLFHNEWVLLVVCEASEGAFYHYDIMRGWQPVSGLPEQSSAAAPRFVG
                     ESAPFLHQKDGETVGSVQEATAFEQKRMNP"
     gene            complement(58596..60260)
                     /locus_tag="PLS35_00265"
     CDS             complement(58596..60260)
                     /locus_tag="PLS35_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNI17104.1"
                     /translation="MMEAIFPLVTILAPLFGTLSIALFWREMGERVSRLGIGALWASA
                     LTSLVTLALVSYGDPIRLTLAGAPSGAWSYTILVDRLAAVMMVLISSVSLVIHVYSER
                     YMVGDAGYTRFFALLGSLTFVLLSLVSSGNLLWMFACWHLVTWLLATLLVCNPASLAA
                     RDAGQKTFWVQSLGDAAFALVLLILYGSYGTLDVTELFPRVQAAPVTSWSGLALELNV
                     PLVTTLLLVLSVMTKSAQFPFHFWLIGTIEAPTPVSALMHAGIVNAGGFLVNRMAPLF
                     GLAPTTLHLLFVIGGLTALIGASAMLTQSSVKRTLVYSTMGQMGYMVMECGLGAFALA
                     VFHLCAHGLFKATLFLNSGAQIHKARTEFKLPPGNRVEESRAFSPMTWTTGLAVTLVL
                     PLIILLVAHGVVSIPLQESQGTVIFLFFGWVTTSQAMFSLYRLHAGASWNVSLTMLGA
                     LALIGLTYLWAGEAFTHFLYPAHGIAASYVQAAEWNRALFDAVVGVSAIMIVGAWGLI
                     YGKAKGIKVLMPGWMESLRTRAYVAFLNGLYIEDMVRMIGRAGSRR"
     gene            60632..61129
                     /locus_tag="PLS35_00270"
     CDS             60632..61129
                     /locus_tag="PLS35_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17105.1"
                     /translation="MLSAGQIRTSERRRIAHVLRGWMILWLLALPLFHIHPETDPHHG
                     EAGHVHAAAVHTVFSGDLEGEFGEHQETASVRPHPGEGPAVSAEGAHAWSSDPELNLS
                     LLNDATDRKLAKPLLTHLLFVAHHLVPLFDQQDGPHGEPLSSTVTMLFTRDVPARAPP
                     SPFFS"
     gene            61233..62483
                     /locus_tag="PLS35_00275"
     CDS             61233..62483
                     /locus_tag="PLS35_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI17106.1"
                     /translation="MMPRMVLSLLMACVFSQSGALSWAGEERPRAYSLSDVLTFAAQH
                     NPVLAGAEGLVKQSQGQQIAAGAYPNPSITGIAGRGAIRDPSTGTHVTERTLTVEQPL
                     EWTGKRQARQEAADAGLAGANAALEDARLSLLADVKIAFYHLLFGQRDVELSAQNVTN
                     VEEVLRTVQARVTAGEATSFDSLKAGVEVQKAKKEVARANNALLVARARLNTLTAGTL
                     GKEFSVQGDFDRPKQSLNADGLTAQALEQHPAVRRLSKLAEQADHTLRFEREARVPNV
                     SVIGSYHREAGDESLTAGLAVPLPLWYRRQGEIQSALGAKHRADAERLRAQQELEQAI
                     TQHAQEVRTAQEQLQVFETGLLKQAEQTLTVARTSFRHGAASLLDVLDAQRVYRQTQL
                     EYAQVRADLSIALARLERALGTSL"
     gene            62521..63684
                     /locus_tag="PLS35_00280"
     CDS             62521..63684
                     /locus_tag="PLS35_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI17107.1"
                     /translation="MTRRLMTIGMVPALLWLLSGCSDQGTPPPKPATTGEAQTITGSS
                     KAIHPPPAVQARLRSEPAALRAVPTLITAPGEVALDLKQVAKITSRIGGQVERIHVQL
                     GDRVKKGQALVAIGSLQLDQLIEEYLVGKAQADVAENSFRRTEKLRADDIVTERKLIE
                     DKGLYLETQARYQHIRERLSNMGISTDDLKQGPHEKSHLYTLTAPIAGTVVIQNAVRG
                     QGVGPGDELFEIVDTSRVWVFANLPIEQARQFKEGDSGTITPKGGEAIVAPLTYLSPV
                     ADETTRTIRVRFEVANLQGQLKPREYVEVALGWSGAPVITVPMTAITTIDKTRGLFLD
                     TADGYTFVPVDAGREGGGWVEIRRGVKEGDRIVTDGVFDLKNVLLKEHIGSGE"
     gene            63692..66814
                     /locus_tag="PLS35_00285"
     CDS             63692..66814
                     /locus_tag="PLS35_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNI17108.1"
                     /translation="MQQLLTFSLRYRFFTLVAIVVVIASGLWSFAHLTIDAVPDLTPV
                     QVQILTRAPALGPVEVEQFVTFPIEASLNGLPALRELRSISRYGLSAVTAIFDDRTDI
                     YRARQFVAERLAQAMERIPAEYGRPVMGPLTTGLGEVYQFTVKGEGFSPMALRTLLQW
                     DIGMKLRAVPGVVEVNIWGGDPQQFHVIVDPSKLLSFKLSMKQVFDALQRNNAIAGGG
                     YIEHQREQLSIRGEALATQLSDLAQIVVAHGPGGVPIYIADLAEVKEGSALRIGAATA
                     MGEGETVIGMVQMLAGENAQEVVTRVKARVQDIQATLPAGVTIEPYYDRTIFVSKVMQ
                     TVRNNLLEGGLLVIAVLFLFLGDLRAGLIVASAIPLAMLIAFIGMMQAGLSGNLMSLG
                     AIDFGLLVDGSVVMIDNILRRLGEKPPPTREARLKEVLAAGQEVLRPMTLAVTIIILV
                     YVPILALTGIEGKMFRPMAVTVIMALTGSLFLALTVTPLLSFWFLRATPGSETTAVIR
                     GVRRVYAPWLQRAVARPIWPVSIAVGLFAVSLVAGSRLGIEFVPRLEEGDLAVQVWRL
                     PSISLSESVTTALDIERVLRRFPEVTRVVTRTGSPEVATDVMGVEMSDVFVILKPQRD
                     WVTAHSRNELIEAMKRQITDQVPGVGLGFTQPIEMRFNELIAGVRSDLAVKIFGPDLD
                     ILKQQAERAARALQHVPGAADVKVEQVAGLPLLRVVVDRAEIARYGLTAEEVLTLVQS
                     ARVGTIVGTVVQGSRRFELVVRLADRVSRDPGSLGSLLIPTMHGELVPLSRVTSILVD
                     SGPAQVSREHVQRRIVVETNIRGRDLGGFVSEAQQAVAQAVTLPPGYELIWGGQYEHL
                     QEAGKRLAMVVPVTLMLILGMLSVIFGTLRPALLIFLNVPLALSGGILALWLRGLPLS
                     ISAIIGFIALFGIAVLNAVVLVSHIRQLEAEGVSTREAVLHGAMDRLRPVLMTALVAS
                     LGFLPMALATSMGAEVQRPLATVVIGGLLTSTALTLLVIPALYSRLVPQAASRPHPAA
                     VQADDSAIQV"
     gene            67007..67372
                     /locus_tag="PLS35_00290"
     CDS             67007..67372
                     /locus_tag="PLS35_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17109.1"
                     /translation="MATIMVIDDEPSIRGLLREVLERSGHKVVEAKDGREALDLYQKH
                     KADLLIMDLLMPEVDGLEATLQLTREYMDTKIIAMTGAQGDRNFLDIARLFGAHRTFE
                     KPFDLKEMLNAVEAELAQK"
     gene            67545..67943
                     /locus_tag="PLS35_00295"
     CDS             67545..67943
                     /locus_tag="PLS35_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNI17110.1"
                     /translation="MLIVLVGILPLHNFVGHAHWQYIRWVPTPDQLSSPAILLDLAFD
                     AVANILLFVPFGLLYALRGLEGKPLSPRHLLLMAFALSFSIEYYQVYCHNRSTSLLDL
                     LDNVLGAYLGMKAGEVYIRKNASAVVEPAA"
     gene            complement(67946..68353)
                     /locus_tag="PLS35_00300"
     CDS             complement(67946..68353)
                     /locus_tag="PLS35_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNI17111.1"
                     /translation="MACLCVLGLVLSGCAAPKPILYPNAHYQQVGADAAQADIQDCMA
                     LAKEAGASSSEGKTAQVVTGTVGGGAVGSAAGAVGGAILGHPGRGAMVGAASGATAGF
                     LRGLFKRSPPGAAFTNYVDRCLRDRGYDPTGWQ"
     gene            complement(68433..69290)
                     /locus_tag="PLS35_00305"
     CDS             complement(68433..69290)
                     /locus_tag="PLS35_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17112.1"
                     /translation="MFKIKKKIEKPKPPILYNVIEDYSEFDAPGNVTVCAMTLPEDLA
                     AHAKILAESYDVPLEQMLQLLTDGGVYVYPQVGGLLTRGLFACRVDPTGASPKQTWVL
                     DLMQFAEAEQWARARAVSLIDAAGEIFYRTLPEELKTKLAQKNLGLDYRTLDRAVAKG
                     GDIKFIDFRKDWSPHFKRLCIMPDGRLVETGGLEEFAQLHQITMPQAKKLVEEGGTMD
                     VAGGEVLACQIVNGQPAVARFSAKNYAKAKELVTAKGLHIMDALSEVAYNDSVMMRTL
                     QRKDLGGRI"
     gene            complement(69356..71101)
                     /locus_tag="PLS35_00310"
     CDS             complement(69356..71101)
                     /locus_tag="PLS35_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNI17113.1"
                     /translation="MTLDQPSSGASAGTTSTDPIDRVIAYHIRTKHHFNRYARSLGFL
                     DWANQPNPFRRFEGAQLIRLPLLSPDDDPQSPSYDALYQPGAVASRPLTIKTISRFFE
                     LALGLSAWKRAGESEWALRNNPSSGNLHPTEGYVIVPPVDGLDLPPGLYHYAAKEHGL
                     EFRAGLPSGKITQLLAPFPDGAFLFGLSSVHWREAWKYGERAFRYCNHDVGHALGSAR
                     IAAATLGWRMVLLDGVEQNQVARLLGTHRQEDFGEAEPEHPDCLAVIWPSPNMKHDVQ
                     NLPLGVDQTVVKDLAPGPWHGHANLLSREHGVHWEAIDEAAEASWKTGEERLVVSLPV
                     EPPVHSEPRHDPQATRGAATIIRQRRSAVSFDGRTAISSATFFQILLRLIPHASQPQL
                     ARPMPWDLLPWDPAIHLLLFVHRVEGLTPGLYVMARDAKKLPLLQQAMNPELEWTPVP
                     GCPTDLPLYWLLQGNAQRLAAQVSCHQGIAGDSAFSLGMLAEFEGRLRQGGAWWYPRL
                     FWEAGLVGQVLYLEAEAAGVRGTGIGCYFDDPVHEIVGIKDLTLQSLYHFTIGGPVDD
                     GRLLTLPAYYHLQRG"
     gene            complement(71150..>71860)
                     /locus_tag="PLS35_00315"
     CDS             complement(71150..>71860)
                     /locus_tag="PLS35_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNI17114.1"
                     /translation="GRSIPMPATPHSEKHFTATATVRDIVIGMADGLTVPFALAAGLS
                     GAVASSALVVTAGLAEIAAGSIAMGLGGYLAAKTDLEHYAAERKREVRETEHIPETEA
                     EEVAEIFRGYGLRDEHIAPLIASLRANPRRWVDFMMRFELGLEEPDPARARSSAWTIA
                     LSYVIGGLIPLLPYMVFHEIATALWWSIAVTPVALFVFGYVKSGLTGVPPWRGGVQTV
                     FVGGLAAAAAFGIARLLG"
BASE COUNT        14867 a        22066 c        20016 g        14611 t
ORIGIN      
        1 ccctcggtac cgacttctct cgcgacccgc gtcacctcgc tcgcaaacgc gctcagctga
       61 gcgaccatgc gattgatcac gtccttgatc tgcagaattt ctccgcgcac atcgaccgtg
      121 atctgcctgg tcaagtcacc gttcgcaatc gaggtcgcga cgttcgacat atcccgcaat
      181 tgcaccgtca ggttgctggc catggcgttc acgctgtcag tgaggtcttt ccacgtaccg
      241 gcgacacccg gcacgaccgc ctggccgccc agtttgcctt ccgtgcccac ttcgcgcgcc
      301 acccgggtca cttccgccgc aaaggcgttc aattgatcga ccatcgtatt cgtcgtgtcc
      361 ttgagctgca gaatctcgcc gcgcacgtcc accgtaatct tcttcgacaa gtcgccgctc
      421 gccacggcga tcgtcacctc ggcgatgttc cgcacctggg ccgtcagatt ccgcgccatg
      481 ctgttcacgc tgtcggtgag atccttccag gtcccggcca ccccgggcac aatggcctgc
      541 ccgccgagtt tgccgtcggt gccgacttcg cgcgccaccc gcgtgacctc ggccgcaaaa
      601 ctccgcagct ggtccaccat cgtgttgatg gcctctttca actgcaaaat ttctccatgc
      661 acgtccacgg tgatcttttt ggacaagtcg ccgttggcca ccgcaatcgt gacattcgct
      721 atgttccgca cctggtcggt caggttgccc gccatgagat tgacgctgtc cgtgagatcc
      781 ttccagacgc cgctcacatc gcgtacctgg gcctgcccgc ccagcttgcc ttccgtgccc
      841 acttcccgcg ccacccgcgt gacctccgcc gcgaaactcc tcaattgatc caccatcgtg
      901 ttgatggctt ccttgagttg cagaatttcg ccgcggacat cggccgtaat tttcttcgac
      961 agatcgccgt tggctaccgc aattgtgaca tacgcaatgt tccgcacctg gtcggtcaga
     1021 ttactcgcca tggagttgac gctgtccgtg agatccttcc aggttcctgc gacgcctgat
     1081 accgcggcct ggccgcctaa cttgccttcc gtccccacct cgcgtgccac ccgcgtgacc
     1141 tcggcggcga agctacgcag ctggtccacc atcgtgttga tggcttcctt gagttggagg
     1201 atttcgccgc gcacgtccac ggtgattttt ttggagaggt ccccgttcgc caccgcgatc
     1261 gtcacttcgg caatgttgcg cacctggtcg gtcagattgc cggccatgag gttcacactc
     1321 tcggtcagat ccttccagac accgctcaca tcccgcacct gcgcctggcc gccgagtttt
     1381 ccttccgtgc ccacttccag agcgacgcgc gtcacttcgg ccgcgaagct gcgtagctgg
     1441 tccaccatcg tgttgatggc ttctttgagt tgcagaattt cgccgcgcac gtcggccgta
     1501 atctttttgg acaagtcgcc gttcgccacg gccgttgtca cctcggcaat gttgcgcacc
     1561 tgggccgtca ggttactggc catcgaattc acgctgtcgg tgagatcctt ccacacgccg
     1621 ctcacatccc gcacctgcgc ctggccgcct aatttgccgt cggtgccgac ttcgcgcgcc
     1681 acccgcgtga cctcggccgc gaaactccgt aactggtcca ccatggtatt gatggtgtcc
     1741 ttgagctgca gaatttcccc gcgcacgtcg gccgtgattt ttttcgagag atcgccgttg
     1801 gccacggctg tcgtcacctc ggcgatgttg cgaacctgat tcgtcagatt gctggccatg
     1861 gagttcacac tatccgtgag atccttccac gtcccggcca cccccggcac cacggcctgt
     1921 cccccgagtt tgccttcggt gccgacttcc cgtgcgactc tggtcacctc ggccgcgaag
     1981 ccgcgcaact ggtcgaccat cgtgttgatg gcttctttga gttgcaggat ttcgccgcgc
     2041 acatcagcgg tgattttctt cgaaaggtct ccattggcga cggcggtcgt cacatcggca
     2101 atgttgcgca cctgggccgt caaatttccg gccatgaggt tgacgctttc cgtcaggtcc
     2161 ttccagacgc cgctgacatc gcgcacttgg gcctggccgc ctaacttgcc atcggtgccg
     2221 acttcgcgcg cgacccgtgt gacctccgcc gcgaaactac gtaactgatc caccatcgta
     2281 ttgatcgtat ctttgagctg caggatttcg ccgcgcacgt ctacggtgat ttttttagac
     2341 agatcaccat tcgcgacggc caccgtcacg tcggcaatgt tccgcacctg gttcgtgaga
     2401 ttgctggcca tggaattcac gctgtcggtg agatccttcc aggtaccggc cacgccgggc
     2461 accaccgcct gcccaccgag tttgccgtcc gtccccactt cacgcgccac acgggtcact
     2521 tccgctgcga aactgcgcaa ctggtccacc atggtattga tcgtgtcttt gagctgaagg
     2581 atttcgccgc gcacgtcggc ggtgattttc ttggataggt cgccgttcgc gacagcgatg
     2641 gtgacctccg cgatgttccg tacctggttg gtgaggttgc tggccatgga gttgacgttg
     2701 tccgtcaagt ccttccaggt tccggccaca ccgggaacca cagcttgtcc gcccagcttg
     2761 ccctccgtac cgacctctcg cgcgacccga gtgacttcgg ccgcaaaggc atccagctga
     2821 ccgaccatac tgttcaccgt tttggcggtg ctcaggaatt cgccccgcag cggatgtccc
     2881 tctgcttcca gggccacgga ttgagtcagg tctcctttgg cgaccgcccc gatgacacgc
     2941 gccatttctg tggtcggccg gaccaggtcg tcgatcagac cgttgaccgc ttcaatatta
     3001 gttttccatt ctccccgggc ttcaggaacc gacgcgcgtt ccgacaactt tccttttttg
     3061 cccaccgcat ggctgacccg cttcagctcc tcagagaaca tggcgttcaa ttcaatcaag
     3121 ccattgattt catccgcaat ctttccagcc atcccatccc actccgctgg caggcgcaca
     3181 gaaaagtcgc cctttttgac ggcttggaga atgttgagga cggccttgtc atggccctcc
     3241 tcatagagga ccccggactg ttgtcgcttg ccggatcgcg aacgcagctt ggtattcatt
     3301 gaatccctcc gtccatcact gcagagcagc gccgcggtcg agaagacgac ccacgggtta
     3361 gaatcactgt agtgcgaaac actataaagg gcgggattat ccgcaaggac tgggggatac
     3421 cctagttccc taggcttggg attgtcgcag agctttgtag gaagaaatcg gattggagag
     3481 gagtcgtgag gcggaggatc aggctgaaca gacggctcga atactgggct cacggtgccc
     3541 gccgcccaag gtcaggcatg cgggctctcc gggtgcgttc gttgatttac cacatgacaa
     3601 ttgagcgcca gcgatgtcct gcgtccgccg tcggaatgga aattctggca atcccgccgg
     3661 tgaccgtcac ccgagcgtgc ccaatatagg cgtcagtggg aatgggaagg tatcgttcga
     3721 ccgttccgcc ggtgaggata atgccttgct gcgcgaattc cacggcgacc tgcatggatg
     3781 tattctccag caccggtcga aagtctgcat agacattaaa gttaccatcg ctttcggaca
     3841 cttcgatgac gggttgatgg agagaaagga ttgcggtcgc cccgcagtat gaagcggact
     3901 gaccgagtga agtccctaag acggcacggc ctacgtttgg ttcctggatt ggacgattca
     3961 attcatttat catggcggct cctttatgaa tggaactgat gattgccctg tgcttggtac
     4021 cttagcgggt tttcccagac gagttactag gaactcccct tgattcagag tcgatgtccg
     4081 ctagcacttt gcctagttgt gacacagctg tcggcggctc tagagtggcc tgaaaaagtg
     4141 gtgtcgcgct tcccctactc tggaactcag gaatatgtat atgccgagtc ctgacgaaac
     4201 atccccttcg atccctcaac ccggttttcc cttggatttc atgctagccc atctggcagc
     4261 catcgtggaa tgttccgacg atgccattat ttccaaggat ctcaacggga tgatcacgag
     4321 ctggaaccgg ggcgcgcaac acctgtttgg ctacacggcc tgtgaaatga tcgggcagcc
     4381 ggtgctacgc ctgatccccg aggatcgcca ggccgaggag ccacgtattt tggagcggct
     4441 ccgccgggga gaacgtatcg atcactatga aaccgtacgg aaacgcaagg acgggacctt
     4501 aattgaaatc tctctctccg tctcgcccat caaggaccgc gagggacgga tcatcggggc
     4561 atccaaaatc gcgcgggcga tatcggaact aaagcagggc gaacggaatt tagcgcatct
     4621 ggtcgaggcg cttcctgccg cggtctatac cacggatgct gacggccgca ttacgcggta
     4681 caaccaagcc gccgtcgatc tctgggggca gcggccttcc ctcgagaccg ctcgctggtc
     4741 cggatcctgg cgccaatact gggcggatgg acgccccatg ccaccggaag aatgtccctt
     4801 ggcgcaatcc cttgccctca atcgccccgt aagcggattg gagaccatcg tcgaacggcc
     4861 ggacggcact cgcctgccgg tggcctctca ccccacgccg ctgcgggatg catccggcgc
     4921 tcctatcggt ggagtggata tcctcgtaga tttgaccgag caaaagcggg cggaacggga
     4981 catgcagaat tccgccgctg aactcgaacg ccgcatcgtt gaacgaacag gcgagctcct
     5041 tctctcgcag gaacgcctgc gcgcattggc ctctgacctc acgctgaccg aacaacggga
     5101 acgccggcat ctggctaccg aacttcacga ctatctggca caacttgtcg tcgccagccg
     5161 gatcagactc gcacaagtca ttccgaccat tcacgatgcc acggcatcgg ccgccctcac
     5221 gaagatcgat gggatgctcg accaggcgtt gacctatacg cgttccctgg tcgccgagct
     5281 cagccctcac atcctgtatc aattcggctt gtcacaatcc ctggtgtggc tgggagaaca
     5341 gatgaagcag cacggccttg aggtggccgt gaaaactatg gctccgtcat tcaccctgcc
     5401 ggacgatcaa gccgtgttgt tgtttcagtc ggttcgcgaa ctgctcttta atgtcatcaa
     5461 acatgccaaa gtcgaccgcg ccgcgattac cataaacgtg gatgaggacc gtcgagagtt
     5521 gtggatctgc gtggaggatg aaggcgccgg cttcgatgtg acggacctga gcgtcgcaaa
     5581 aaatacacgc tgtaaattcg gcctgctgag catcagagaa cggatggagc tgctcggagg
     5641 cgagtgcgaa gtgtcgtccg ttcccggaag cggcacggtc gccatccttc ggcttccact
     5701 ggcatcaggc gccctcaagc agccgtttcc gctggccgcc gacacggcac agaccatcac
     5761 gccttctata gacgcctctt cgcgcaacat taacattctg ctcgtcgatg accatgcgat
     5821 ggtgcggcag gggcttcgaa gtatcttgga cgcacatgac aatttgacgg tggtcggtga
     5881 aggagccgac ggtcaggacg ccgttgttat ggcgcgctcg cttcgtcccg atgtcgtcat
     5941 catggatgtg aatttaccga tcatcgacgg gatcgaggcc actcgcatcc tgacccagga
     6001 acatccctcg atcatggtca tcggcatttc ggtccgcaat gatccacaag tgcatgacgc
     6061 catgacggag gccggggcgg tcgcgtttct accgaaggaa tccgctgctg accagctcta
     6121 cgaagtcatt ctgcgcgcat ttcccgtcca ggcctgagtc cgacgtgagg ccgccgaact
     6181 aggggatccc ccagtagcgt cccgcagccg acagtcctat gctgtaagcg gcttgattga
     6241 atggggattg ttcgcccacg aaacacccat gccactcacc gctgttccac cttgagtcac
     6301 gcccggagag gggtccgttg tcgcttgtcg aaggcggtaa gacccggagg cttgtcattt
     6361 acacatgact tcggtgctgc tgacgctgca acgtctggtc ccctccgccg ccggactcgc
     6421 cattggattt ccggtcttcg cctttctttg ttgggcatac aacccgacat tcctctacaa
     6481 cctgtcgccc tcggcaggaa cggttaaacc atccacgatg gccgccatat gtttctgcgg
     6541 cctctccttg tggctccagg ccgatgaggc cgcgatgacg cgagcgaggc ggcgcatggc
     6601 gcaaggcttt gccggcctcg ccggccttct ggccgccgtc acaatgttgg aatatctatt
     6661 gtccctcgac ctgggcgtcg atctgtggct cgcctctgtt ccggatttgg cggtggactc
     6721 ccatcccggc cgaatggctc ctgccacagc cttcgctctt ttcatgctgg gcttggctct
     6781 gatccagctt gattcgaaac acagcggcca ggaccaggcc agtcattatc tggcacttgc
     6841 gggaggcacc attggggcca tcgcactggt cgggtatctg tacggtgtcc agacgctcta
     6901 caaggtcggc acatccaata ccatttccat ctttagcgcc gcgctgctcc tcgccctcgg
     6961 cctgggtatt ctgagcgccc gccccacgcg cgggtttatg gccaccttaa ccagcgacga
     7021 actgggggcg gtgatgctgc ggcgcgtcct gcccttcgcc attgccctgc cgattctggc
     7081 cggatggatc cgtatcgcgg ctcaacggtc gggccgctac gattttaact tcggggtcgc
     7141 gctggccgtg tcggccgtca tgttgatcat cctggccttt ctctggttat tggccgggaa
     7201 tctcaatcgc agcgacgggc aaaagaagca tatcctgcaa atactgcagc aacgcgaggc
     7261 ccggcaccgt ctcgcgctga aggccggtcg tatgggaacg ttccaccagg atcttgatcg
     7321 tcacaccctg gtcttttctc ccgaactgga acacatcttc ggtttgcctg cgatgagctt
     7381 cggcagtacg ttcgccgcat tccaggatct ggttcatccc gaggaccggg cacatgtgct
     7441 caagaccatt aactacgcga ttcaacaccg gagcgcctac gacctcgaat gccgcatcct
     7501 ccggcaggat cctcccgagg aagcctggat cgcagtcacc ggacaggtgt tgcctgactc
     7561 caccggccat ccgaggcaaa tcaccggggt catgtttgat atcacggaac gcaaacactc
     7621 cgtgaccgct ctccagcaga gtgaacagca gctgcgggtc atcactgatg cgctgcccgg
     7681 ccttattgct tctgtcgatt gtgacgagcg ataccggttc gtcaatgcgg gctatgagca
     7741 ccagttcgga ctgtcgcgtg accagatcgt ggggctttcg gtgaaggatc tcctcaagga
     7801 ggactattcc accgttcagc ccttcatccg gcgcgtgctg agtggtgaac gcatatcctt
     7861 tgaaactacc ctcctggatg agcggggtga gcagacgctg ttatcgacgt acgtgccgga
     7921 ccgaaactcc gaaggcatgg tggcgggatt ctatgcgctg atcacaaata tcacggagcg
     7981 caaacgcacc gaatccgcgc tgagagaaag cgaagaacgc ttcaggcatc tctttgagca
     8041 ggcgtcggac gggattgtga ccgcggacat gaacggccag tatctcgatg tgaacaatcg
     8101 cgcctgccag atgttaggct atacccgcga agagctcttc gacctgcgag tccccgacct
     8161 gctggacccg catgactacc cgcgattgag agcgatcaag gccgaactga tccaaggaca
     8221 tcgtcattcc ggcgaatgga aattacgccg caaggacggg ggaaccatct ccgtcgagat
     8281 cagcgcgaga ttgcaaccgg accgccgctg gcatgccatc gtcagagaca tcactgaacg
     8341 caaacgggcc gatgcgggat tgcgcgaaag tgaggcgtat tttcgcatgc tggccgatgc
     8401 cactccggtg ctggtctgga aagccgggac ggatcggctc tgttcctggc tcaaccggtc
     8461 ctggttggag tacaccgggc ggctgttgga tgatgaattg ggaaccggcc ggaccgacag
     8521 catccatccc gacgaccggg acgaattcaa cagggtctac aaccagcact tctctcgcca
     8581 agagcctttt gaactggaat atcgattacg ccggcaagac gggacctacg gatggatcct
     8641 ggaccgcggc gttccgctgc ttagcgccgc cggggtgttt agtggctatc tcggcgcggc
     8701 cctcgatatc acggaccgga agttagcgga agaacagctg caacaatgga ccgtcgaact
     8761 ggagaagcgc gtggacgagc gcacgcaagc actcgtgcat tcgcaagcgc gtctccgtgc
     8821 gctggtatcc gatctcagcg ccacggaaga gcaggagcgt cgccggctcg ccacggagtt
     8881 acatgactat ctcgctcagt tgctggtcgt cggccggatg aaactcagcc aagcgagacc
     8941 gcaggtccgg aatcccaaaa cggaacaact gttgagtgaa gcggacgaca tgctcacgca
     9001 atcgctcaac tacacacgat ccctcgtcgc tgaattgagc ccgcagatcc tgtatcaatt
     9061 cggtttgcct gccgccctca aatggctggc gggacagatg aaatcacacg ggctttcagt
     9121 gtccatccaa tgcggcatcg atcagctcac gctcgctgaa gaacgcgcgg tcattttata
     9181 tcagagtgtc cgggagcttc tgttcaacgt ggcaaaacat gctggaaccg gcgaggccat
     9241 gatcacgctg gaccagagtc cgaccaatca ggtgctgatt actgtggcgg acaacgggcg
     9301 cggattcaat ccagccattc ttgtagacac cgagggccat catcctggac ggttcggact
     9361 ctttaacgtg caagaacgca tcgaagcgat cggcggaaca ctgagtctca aatcttctga
     9421 aggctgcgga acgacggtca cactgattgt gcccctgaag ccctttccag aatcggctgt
     9481 cgagtctgac ctccccgtcg acggccatgt cccggatgtg ctcaggaccc cgtctcaacg
     9541 attacgcatt ctgctggtcg atgaccatgc catggtgcgt caaggattgc ggagtgtatt
     9601 ggaaagttat accgatctgg aggtggtggg ggaagccggc gatggaggga ctgccatcag
     9661 tatcgcctcc gccttaaaac cggatgtggt cgtcatggat atcaatatgc cgaacatcga
     9721 cggcatagag gcgactcgcc atatcctcag cgagcatccc gatgtcgtgg tgatcggcct
     9781 ctcggtccaa aacgaacggc atatcgaaga agccatgttg aaggccgggg ccgcggtctt
     9841 tgtcaccaaa gaacgggccg ccggccaact ctacgaagcc attgtgagca ccgtgcgaac
     9901 cagacgatga gcagttcgca cgggtcacac agctggcggg cgtcggccca tcaccatgcc
     9961 gatcacggcc aaaatcagaa agatcacgaa caggacatac gcgatatgcg tggcggttcc
    10021 agccactccg gtgacgccca gcacacctgc aatcagcccg atcactaaaa aagtcacagc
    10081 ccagctcaac atgatcgcac ctcatctata ggtgaaccca tactccctta tctatcggga
    10141 gccccgctcg aatggtacgc gtgctgcgta ccacaccctt tatgcccgca gacacaactt
    10201 ccggtcggcg ctgcggcatt tcgacaggca tcggaacaaa actcacggcc tttttcggcc
    10261 tcacaagcac aggcaggatg cgcgcatgat ttttttcgta tggttgcctc cgccattacc
    10321 tcgtcctttc cggcggccga ctccgccttc cttctcgagc ctcttccctt tgctgatgtt
    10381 agaaccacag gtgaataccc atgccgatcc actccacccg ttgagtaaac aattgtccgt
    10441 ccgggctcgg accggagtaa tactcgccca ggacctgcaa cttccgatcg ccgatccggg
    10501 cattttcaaa ctgcaagccg gccataatgg actgaccggt cgtccaatgc gttctcgcat
    10561 gggtttgaaa atcggcgtac gccacaggcc tcacttttcc cccgaagaag agccacggac
    10621 tcttgagctc gaccccgcct tgagtcgttt cccgccccaa cgtgctcggg tcttttctca
    10681 caatcgttcc gacgccgccg tacacccgca accaggtcgt cagatcgtat gacatcttga
    10741 gatcgatctc ctcatagctg aggttgatcc ggacgacctg gggattattc agtaaaaatt
    10801 cgtctcctaa atgtgaactc tgatgccgaa tacgaatgaa cccagagaat ctatcggccc
    10861 gatagctggc cacaatgccg aaggtgtagt cggcattgat caaatcgatc gagcgctttt
    10921 ccgtatcgaa tacgccgaac acaccggctt gaattcccag ttcccattcg ccctgaaaag
    10981 gagcggcatc ccggtacacc gagaaggatt cgccgaaggc actggcaaat tcccggccgc
    11041 ctgatgaaag agaatggtag gaggcgccga aatgcggcca tcgtaaatcc gcgtgaaacg
    11101 ggtccacgag caatccgcgc ggaagaaatt tcgattccgc ggtcggtgtc gtcgcacgga
    11161 ccacgcccgg ccccgccgct ccttccggcg cagaccccgt gtgtacctca gcgctaacaa
    11221 ccccgggaat tgccttgaga gccgtgacga tcttcgccgg gtcctcagcc gccaatcgct
    11281 tggcatacac cgccacccgc ccctgttcga cctgcaccac cgctccagag agatggaact
    11341 catgttccag caggccggcc gcgtatccgg cgatatactc gtcggacgcg gccatcgcca
    11401 ttcccgcgaa tcccgccatc acgcccagga gaaagagggc catcccaata ggcgatgtgc
    11461 ggctcctagt cccctctttc ggacacctgt gccacaacct gttccacctc acagctccgt
    11521 ccgtctcagc ctagaaatgg aagttgacac cgaccgtcac catgtggccg ttgtcctgga
    11581 actttttgtc gacgatgttt tggtcccggg actcgatttt ttccagccag atgtaccggt
    11641 acgtactgtc gatggataca tgcttgttaa agaaaaattg gagaccgccg ccggcgtgag
    11701 cgccaaaccg gttttgcgta tcatcgaagc cgcccggccc cttcacggtc gtgtaatacc
    11761 acccgccgcc acctaacaag aatggcgcca gtctggtggt gcccagtgga taaatcagag
    11821 cggagacttg aacggggtag gtatggacgc gcgtatcacc gatgtcgttg cggcgatagt
    11881 ctgccgagcc ttcaaatgcc aggtaccgcc agggatgcaa tcgaacctgc ccacccccga
    11941 accatcggct tgagccgtcc ttcggatcaa tgtatgtggc gcgaccaccg atggagagca
    12001 gcccgatgtc catctgctca aacaaccctt tatcgtccgc cgcccttgct ggggacggca
    12061 cgaccactcc gcccgcgatc aaaaacgatg cacacaggac caacgctgcg ctcaagaccc
    12121 cttgtctcat tgtcgatctc ctctgtaaat ggcaatgtgg cctagttgac ttcgctagga
    12181 ttgggtcctc ttctccccat ggctctcaaa ccattcatca acccagcgtt tcacttcttc
    12241 ccgccgatcg ccataccgct cctggatctt gccttccagc gtatcgatgc gcccctcgat
    12301 gagcatcaag tcgtcatcgg tgaactcagc ccacttctgt ttcaattcac ccatgaattg
    12361 tttccacatt cctgccaatt gatcctgatt gacggccaag atcagggtgc cctgtgtgcc
    12421 acgtaacctg tgttgttcgc ccgcatgtcc cgccgccgtc atcatcagaa tccccgcgag
    12481 ggcgacgatt gacagcctcc agtgcccgag cctcatcgtg tctctccttc gaagccgatg
    12541 gcaaggcgca ccggcgtgtc acctgccggt acgcctggtg cccctcaacg gtctattttc
    12601 gcctgaccgc tttgcgcgtc gacatagact ttctgttctt tgttgttcga gtcgatgatt
    12661 tcgacttcat agaccgtgcg gccatcttgt tttccgatct cagcctctac ggctttaccc
    12721 ggaacaagct tcaaggcgtg tttcacggca tcttccaggg tgaccgtggc cgtcgccgct
    12781 aactgcttgt tcgtttcaaa cagagcccag gccgggctgg ctatgacacc ggacaacaat
    12841 gcgatggcaa tcattttctt catagggccg actccttccg cgtcaggttg agctttgtac
    12901 gtatcctcat atgtgttcag cgcgggggct gagtctcccg atgctgagtt ccctgctgcg
    12961 cttgcccttg gcggctgtac caatcatccg cccagcgcat gacttcttcc ttcttatccc
    13021 cataccgctc ctgaatgcgg ccgaggaatt tgtcgtaatc tccttcgacc tgcttcaggt
    13081 catcatcggt caattttccc cactgttttt tgacttcccc tttgaactgg acccatttcc
    13141 ctttgaattg atctgtattc atcgtgatcc tccatagtga tgtatcattg ccgcggacgc
    13201 ttgcgcccct gaggaagcgg cgtccccgcg actcctctgt cgggcggcct ctttcagtac
    13261 ccggtgggac ggagatcgaa aagcaagtcc ggtttttcca cctcggtaca acaccacggt
    13321 attctcctcg gcgttgtcgc cgagggtgac gacgatcttc gtccgcactc cgtggtcttt
    13381 ctgatgtggt ttcataggca cctcccgacg gttgtccatc tttgttatat ctccgaatag
    13441 ggaaatggtt gactggacaa agccctagtt ttaaaaggcc ctcgcccctg acaggtctac
    13501 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattcagat gagggtaaca
    13621 gaaactcgac cggttcatga tgcgcattcg agagcaccag aaatgtatca tccacaattg
    13681 cgttgccatc ggcatgcagc cacacaatgt ccataatgga ggccggggcg cccctccggc
    13741 aggtagacat gccaggtttg attggtccgt tcttcgagac gaatccgatg cgattccctc
    13801 gtcgcatccg gtccatcaaa gaggcatagt tccaccgacg tggtattttc agaaaacagg
    13861 gcaaaattca ccccttcccc atcccacgtg gctcccagcg gatagggtcg gcccggccag
    13921 actttcatat catctccatc cgaaacggac tgtgaatcga gacgtcacag tagcgctcga
    13981 cgtcatcact tcagaagact gaccaggtcc tcggcatgtt cttcttcttt catcagaatg
    14041 gtttccaaca gccttctcgt cgtgggatca tcactgccga caaatgcgat catgtcgcga
    14101 taactctcga tcgcaatccg ttccgcaatg aggtcttcct tgatcatttc ttccaggtct
    14161 tcgccctcca catactcgga gtggctacgg gaagataacc cgtccggcga gagattcggt
    14221 tccccgccta attgaacgat ccgttccgcc agttcatccg catgggcctg ttcctcaccc
    14281 gcatgcgcca ggaactccgc cttgacactt tccgcgttca tcccctgcgc cataaaataa
    14341 tgccgcctgt accgcaaaac acagacgatt tccgtggcca acgcttcatt caagagtttg
    14401 ataaccgtgt cgcggtcggc cttgtaatcc gcggtcaacg cgccttcttc gatatgttgc
    14461 cgggcacgtt ccctgatgga ctgcacatcc ttaatcattg acgcttgagg cttctcagtt
    14521 ttcatgtcga ctcctttcaa cgtaaaggca cggtcacgtg gtggtcagcg cgagaagtct
    14581 gtgcgcacat ggggccaggc cgattccagt cccaattccg tcatcaaacc ctgctcatcc
    14641 gtccgttgcg tcaccgttcg ggcctgggtc ccgaccacga gtgcacaggc agcgatcgaa
    14701 atgagcaatc ccatatactt accgaactga ttgcctgaca gagacaccgg catcatgacg
    14761 tctcctcctc tactgacaac agctgtgctc acactagaag agaggcccag agggacgtac
    14821 taggcagaac ccttggcaac acaggggatt cccctcggcc acggacgagc gaagtcagga
    14881 aggaggaaca taaattgaca acacccccgc caggcggcct tggcgatcca gaaccggcat
    14941 ccaatcgacc tccgacaggc gcatgagcgc ttgtgcatcc gtgagcagat cgtcctcctg
    15001 gcaataggga acctgctcgc tcatgatgtc tctgaccgtc aatgcggtgg tagggggccg
    15061 ggaactccga tgcaatgcga catcccgatc ggtcaccatg cccaccaaac gcgtgccttc
    15121 atagaccacg atcaccggaa cgttcatcgc cgccatcaac gacaccgcct cttcatgact
    15181 cgtgtccggc gacacgacgg tcaaggctga agacatcacg tgtctcacct ttcggacttc
    15241 ccccggcaac actcccaaat cctcatcaat tctggtttcc agatacagac gccgacgcgg
    15301 acgtgtaaag ccggcctcgg caatggtacg aggaacgttc gatttgctgg atgtgctggt
    15361 cattggtgca agattttcaa caattaacaa tttcagacag cttgaggacg agcttcgtgg
    15421 acggtaattc gaagccgccc atactgtacg ggcttgtcgg ggtcccgagt gtaatgactt
    15481 ttatcctccc ccacggagat catgctgaga ggaagcgtaa gatgctgttt ataatgtgga
    15541 aatgccgtct cgagcagcaa cgtcaccgtt tgcccatcgc actcaatcac ttcggcattc
    15601 aggtcgtgct cgttttcgaa atcgacggtc acgcgctcct ccggattgat ccactgccga
    15661 atgcttgccg ccttctctga agattccatg cgccgcctcc gtgtcgccgc atgccgacgg
    15721 ccgtcacccg cgaccgtctt tcgccaacga catcaacact tctcgcatgc attcgctgat
    15781 gccaccactg gcaataactc taggtcctca gccagctgcg gaatcggcgg attgaccggt
    15841 tgcggtggaa tgggtggagg aaacggctcc ggtggaaatg gattcggacc aggatccgga
    15901 ttcggattcg gttcaggaaa cggcgcgggt cctgggttca tctgcgggaa cagattcgag
    15961 tggcgacaat tcatagaagg cctttcattc aatgcggaat agcgacggat gacaatccgg
    16021 cctgaagccg gtcttcaacc acgccccatc cgatattgct aaaaaaagag tcgatatact
    16081 gtttccgctc actgggctgg taatccaaga gaaacgcgtg ctcccacaca tccatcacga
    16141 ggatgggtag aaagcccgcc acattcccat gctcgtgcag agacacccaa tggttcgaca
    16201 cgagccccct tgccggatcg acattacaga tcgcccatcc cacacctcgc atcatgccca
    16261 cactgcaaaa gtcggccttc catcgctcat agctgccaaa ggtacgttca acggccctgt
    16321 gaaaggcgga gccgttcggc ggctccgctc cccctccacg ccgaagattg ccaaaataat
    16381 attcgtgcaa caccatcccg ttgaactcga atcccagcct gcgcgtcagt tccgagtagg
    16441 ccggcatttg ttcgtgatcg acgtgtcctt gttgaatgaa ttcagacaat tgctcgcgca
    16501 attcgtttgc ggcttttaca tagccctcat acagcgcgaa atgaacgcgc aacgtctcat
    16561 cggagatacc cgacaatccg aacaggtcaa acgctttggc ttggtacggt tgggtgacgg
    16621 ccatgttgag cccctttcat cgtgcttaag atggacatac ttcaccatag gcccccacct
    16681 tgcctttccc aactaggcga tgccctggca taccatggcc tacgcagggg gctttcctag
    16741 gcgccatctt tcataaatta cggcagtatg aactgaatgg acccccgaag aatttaccgg
    16801 ggaagagacc gtgcaaagac tagcgcagac ctgctaaggt gaagggaccc tcgaaacgac
    16861 gagagtgtca ccgcggagga gctggtaaag tagtcccacc gagagaggaa ggagcaccgt
    16921 ggccagagca cgcatcgtca ttgccgatga ccatagtctg gtgttggagg cttaccgaca
    16981 attgctggag ccggatttcg atgtggtagg tacggcttca aacggccagg aactgctgaa
    17041 catcgcgccg gccctgaatc cggacattat cctgctcgac atttcgatgc cgaccatgaa
    17101 cgggttggag gtcacgacac aactgcgtgc cgccatacca cacgccaaga tcatcttcgt
    17161 gaccatgatg agtgagccgt tttatatttc gcaggccttt cagatgggcg cgtcagccta
    17221 tgtgttgaaa caatccgctt ccacggaatt gttgtccgcg ctgaacgccg ccctcaagaa
    17281 tcagcgttac atctcgccgc agctctccct tgaggtgcag gatgccatcg aaacgccctg
    17341 ggtgaaaccg gaaggatttt cgtccaagtt gacgcctcgc cagcaggagg tgctgcagtt
    17401 gctgacgaag ggcatgtcga caaaagagat cgccacgacc atgcatgtgt ccacgaaggc
    17461 cgtggaattc cacaaaggca acatcacgcg ccgcctcggc atccgcacga ctgcagaact
    17521 cacgcgcttc gccttatcgc aaggcctcac gaagctgagc gatcaacatc cctgacagat
    17581 gacgttacgg ttgagagccg ggaacggtag gctgttgcgg catggcgatg agcccgatgg
    17641 caatcgcatg tttggtcagc tcggcggtgc tatgaatgcc gagctgtttc atgatgcggg
    17701 ttttgtgaaa ctccaccgtc ttgatggaca cattgagcgt cgaggcgatt tccttggtgg
    17761 actggccttc cgcgatcaac tgcagcactt cccgctgccg gccactgagc gagtcattcg
    17821 acggaggcgc cccggcggga gccggcgcaa ccgggtggag cgcctgggca accagatttt
    17881 ccgccacgct cggtgacacg tacagacggc cctttaagac ttccttaatc gcaaatacca
    17941 attcgtctgc cactgactgc ttcaacacat aaccggagac cccggcgcga aaggcttccg
    18001 tcacgaagta ctgctcgctg tgcatcgtga ggacgaggag cttcacttcg ggaactgatt
    18061 tcttgatttg ccggcaggca tcgaggccgt tcaagagcgg gagtgcgata tccacgagcg
    18121 caatatccgg cttctctttt tcaacggccc ggacgagggc ccgtccgtct gcggctcgac
    18181 cgcacaattg aaaatcgtgt tccagcagtt tttctacacc ttctgcaacg agtgtatgat
    18241 cgtcggctat gaggactcgt ggtttcatgg atatgctccg ggctagtgga caagccgagt
    18301 aggaactgct gtggaatcct accactctct tcagatgaat acgagtggtt cgtcggtcac
    18361 aattccttcg accagaaaaa aatgatgcca tgcccgccct tggtgggcgg cgcgccgcct
    18421 gggtcccgat agatcccgaa gttatacgtg taattgagtt ccacctgcga tttccacggg
    18481 gcccccgtca ccaatccgcc gcccaaggcc ggcatggaat ccgtaatact tttcactgcc
    18541 gtgttcgacg taaacaccgg acgctccacc aatccccagg tcgcctcgac gtaggggtac
    18601 agaaagaata aggcctcata gcgatattgc aagttggcca cggcatagcg ggtcggaaat
    18661 aattcgttga aggcgacacc atggatcatc ggcagcgaaa tggcttccca ctcatacccc
    18721 gtcggccggc cagggaggcg gaacgctgag aatcgatcca ggtccttccc gatccccgca
    18781 tgaatactgc ttaacagacg gtgcttgtcg ctctcgatga atggcagtcc cgtagccagt
    18841 aaggcgtagg cgctggcttg aatgtacgtc tgttcctttt tgaaatcctg agtattgaac
    18901 ggggcgcctc cccattgatc ccacttcgcc cgatgaccat gcaccacatc gccgcccaag
    18961 gtgaatccct cgtgtggcaa ctccatcagg ttgcgcttca gggcatcggc gcgaatccgg
    19021 aaatgaatcc ggccttcata cgtgtccttc ggcacgccgt agtccgcgga ggtgtgaccc
    19081 gtgcccttaa accagcgata tccggcttcg tacgtaagcg aaatgttgac cgcgctgtcc
    19141 tgacgaaagg gatggaccgg cagtcgatac cccagcccgg cgccggcaaa cgcataactc
    19201 cactccaact cggtgcctcg ctgacgttgc ccttgcacat actccgagcg gccgaacggc
    19261 aggatgaaat tatcccaggt gaacaagagc gaccagttcg gatagtcgcg caacccgatc
    19321 gtatagtcca catcgttgac gacccccgaa aaggtcccgc ggagccgccg gttgtcatcg
    19381 tcccaattcc tccagacata taacgcgccg aagggcagta cttcctgttg cgtgggcccg
    19441 ttgggaatgg cctgaatacc gaaactgatg gcggtgacac tgcgccggtc ccggggcggc
    19501 acataaattt cttccccgaa cagggtggtt cgaaacggct cacccgcaat cggggtgggt
    19561 tcctcggcca gcgcgaagtc ctgcccgagc ccaaacagat cgtcaccgat tgcccctaca
    19621 gagaacagac atactatgct gcaactcaca cggaaacacc attcagagag agttcgaccc
    19681 atcgattcta tggctcctga catcgcacgt gggattcctt gacagccgat gtcctcttat
    19741 atgtcagccg tgatacaccc ggcccctagt gaaacccctg gcagaggggg tggaatagcc
    19801 gagttctttt tcgtcgcagg tcgcgcaata catgggaaat ttttgtcgca gccccctttt
    19861 ctcgccaacc tctcaaacaa ggaatattcc cagtgttctc ctgtgtggac caaagatagg
    19921 ttcacgacaa tggaacgtac ctatcgaact cagccacatt gcttcaatgg gacgtctgcg
    19981 catgttcctc gctggcgatg ctgtctcgcc gccgccggat tgttcctcct gacgtcgacc
    20041 acactgttcg cgaacccgat tgaagtcatc tacccggaag gcgttagcga aggatttgtt
    20101 acgctcaaga ccttggacgg caaaaagctc gccgatggag aactgagcca ggtaacgacc
    20161 ggtgccgatc gactggccag ccggctgacc tttcgttttg ccgacggctc gctctatgac
    20221 gaaaccgtca cgttttccca aaagaaacat ctcaccatgt tgagttacca gttgactcag
    20281 cgagggccgg ccttccccga gcccctcgtg atttcgctca atggtgaaac gggacagtat
    20341 caagtccggc aacgcgaaaa gacgccggat gaaaagctga tcaccgggcg cctggacctt
    20401 ccgaaggatg tctacaacgg cctcaccatt accgtcctga aaaatcttcg cggacaaacc
    20461 ggcgccatcg ttcacatgct ggtgtttaac cctgaaccca agctctacga agtcgaattg
    20521 gctcgagtcg atgaggagga cacgcgcaac accaccagga aagagaccaa ggccacagcg
    20581 gtgcattaca gcctcaaacc ccgcttggga tggtttacgg cagcgctggc ccggcttctc
    20641 ggacgaactc cgcccgacta ccacttctgg ctgatcaaga aagacgcccc cgccttcgtg
    20701 cggtttgaag gcagcctcta cgcaggcggt ccaagttgga gaatcgaaca ggtttcgccg
    20761 cgcgtcaaac aagcccgggt tcccgagccc tagtgacctg tcaggggagg cgttgtaacg
    20821 aggcatccaa cgggacaagg gccgctgagt tccggccaat ggccagggcc tccaccgccc
    20881 gcccgaatcg attgatcgtc ccgtttcccc tcaccctcac gtgctccccg acatggaagg
    20941 agcgccggag tgcgggactg gcatcgggtg gaaaccgaac ctgcgtttca tccgacagca
    21001 acatgccttg aatcgtgtgt ttcagcggat ggtagaagaa ggcacgaatc gttcccgccg
    21061 cactcatttc cgtcatcgac agcgtgtgct cctgctcctg catgggcagg tccaggcgga
    21121 gcggaacggt gaacgtcgtt cccgtcgtca cgttgatcac ggcatccgcc tgaaccagcc
    21181 gttcatcggg catgagccgt ccgtagaccc gtacggcgtt gcctggtgcg aacgcccgga
    21241 cgagctgatc cgctgcccgc gaggtgacgt acagatgagt cccgtcggca aggaacaatc
    21301 cttccacctc tcctcgtgga tccagaataa aacggtccac tgttccctct gccgcaaggg
    21361 cgccatgaac ctggtcatgc tgcgcggctg ctacggaaag caccggttcg gccgaccaga
    21421 ccaggaatgg tccagcttct ccacacacga tgccacccaa catgatgccg atcattacaa
    21481 tggccctgcc gttcgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgg
    21601 gctgataccg catgaccagt ccatccaccg tcagctcgcg ttcgatggcg tgcaacgtcc
    21661 cctgcacgcg aggatcgaat gccgggagaa atccgacgag cggaatcatc aataaactgc
    21721 cgtccaagga tgttcccccg taatattgca cgaacgtccc ccgattcgaa tcgtacccct
    21781 ctcggcacac ctgttcgtga atcgtctcac gcaaggctcg ccatcgctcg agcggtccat
    21841 cgacatgaca tcgctcgatg gccttcaccg cccgatccac tgcgacccag gccatcacct
    21901 tggaatgggt gaaatgccgg cgcgggccgc gcacttccca aatgccttcg tcggggcgat
    21961 cccaggcggt ctcaagaaat tccatgatgg catgttggac gcgccaagca tcttcgctgg
    22021 ccgccagccc caactccctg gcctggtgca gggtgtccat catttcgccg taaacatcca
    22081 actgaaactg ctgggaggcc gcattgccga tacgcaccgg ggctgattgc tcgtacccgg
    22141 gcaaccagtt caactccagt tccgtgagtc tccgttctcc cctgatgcca tacatgatct
    22201 gcaggtccga aggtttccct gccaccgcac gcaaaagcca ctcgcgccag gccttggctt
    22261 cgctgtggta gccagccgac agcaaggcat acaacgtgaa ggtcgcatcc cgaatccagc
    22321 agtagcggta atcccaatta cgtacaccac cgagcaattc cgggagcgaa gtggtcggag
    22381 ccgcgacaat gccgcccgtc ggggcatagg tcagcgcttt caatgtgatc agtgaacgga
    22441 ccacatgatt ccgccaggat ccctgatagg tgcagcgttg ggaccatttc ttccaccaga
    22501 gctcggtctg agataacgca cgttccgtga tgagcggtct tgggggagag tcgtgggagg
    22561 cccgccacgt cagagtgaag ggaatctcct gcccttcggt gacggtgaag tcggcgacgg
    22621 tggaaaatcc cacgccctgt aattccagtt cggtttcaag atacaccgcg tcgggaccgg
    22681 ccaccgcctt caggaagcgg cgctgatggc tcacccaggg tacgattgat ccgtaatcga
    22741 atcggagggt caggtccatc ttcatgcgca cggtaccctt gactcccacg acaatacgta
    22801 ccacattgat gtcgcggtca cggggtggca tgaaatcgat caacatcgct gtgcccgttg
    22861 gtgtttcgaa gagcgtttcc agcaccaggg tgtcaccccg ataccgtcga cgaatagttg
    22921 tgttatcttc gtggggcgta atgagccaac gtccatgctc aggcgtaccg agcagcgccg
    22981 cgaagcaggc cggtgaatca aagcgcggaa gacagagcca gtcgagagac ccctggcggg
    23041 taatcagcgc tgctgtgtgg gtatctccga tcagcgcata gtcttcgatt tttgccgtcg
    23101 cgttcatagg cctctcgcgt aggtgcggtg acgtgattga tgattcgttt taaactgtac
    23161 aggcgggcac tcgcctaaac aacccctaac atctcaggag gacaacatga gaaaggttct
    23221 ggcgaaaaaa acgcagccgc gcaaaaaaac ctcttctgcg aaaaagcgca ccgtcaagcg
    23281 ggacatcgaa ccagctgcgc atgtgatcga aagaaaaacg aagaagaagc cggccacatc
    23341 cgccaaggcc aaaaagacca cgctgacaaa gaagaagacc tctaagacaa ccaggaaacg
    23401 atctcccggt tccagccgca acgccaaaag cgtcggtcgg gctgtgggga aagtcatcgg
    23461 ccgagccatc ggaacagtcg aacgggcgct taccctcgtc aggcctaaca gcaatacacc
    23521 gaagaaacaa tagatagacg ggaaaaggaa cagagtcgct agggggacgc ctagtacgga
    23581 tgttgtgggg aaacctctac actgaatcca agcgaacgag ggacgaggac ctcgtgatgc
    23641 gtggaggtgg acccatggca tttcaagtgg tttcgacact ggtgagcacg acgctgctca
    23701 tggctttggc attgcctgcg gcgctgaccg ccgcaaatcc cgctcccgag tcgggccaac
    23761 ctcagccgcc ctcgacccag caaacgccgc cgaccgcccc gccgtcgcgc atcgatccgg
    23821 gcatccaaaa ggctcccgac acggttccca atcccaaatc catcgtgccg ccacctgtgg
    23881 tcgatcccaa gatggcgatc gatcctgaac gaccgggtgg gccggcacat cccaagccgc
    23941 cgtcgccagc agacaagccg cctatgccgc cgcgatgaca tcatacggtc cattctcctg
    24001 aatgcgaggt cggcacgcag gatggaccgt tcatctcatt gcggattgat ccggcggtct
    24061 ttccaataga tcagtcgagc acccggctta aatgtcacgt aataccatat ccactcgaac
    24121 atcactctcg ctcgattgcg aaagccgatg agaaagaaga tatggacaaa gcaccaggcc
    24181 agccaagcca caaccccgga gagtgagatc gggccgaatt gtgcgaccgc cttggcacgt
    24241 ccgatggtcg ccagtattcc acgatcccga tatcgaaacg ctgagcgttg gccgtcggga
    24301 atacaacggg caatgatccc cgccacgtag cggccttcct gcatggcgac ggccgccaat
    24361 cccggcaacg gcttctgctg ggccgaaaga cagtgcgctg catcgccgat cacaaacacc
    24421 cagggatcct ctttgagact gagatcgggg cccactatca cgcgccctga ggaatctaat
    24481 ggaacgtcta gcgaagcgag aatgggtgat gccacattgc ccgcagccca gatcacatgg
    24541 gcgctgctga tgaattctga tccggccatc acgcccttcg acgtcacatc gtgaacgcgg
    24601 cagttcagca gcacagttac gcccaggctt tcaagtgagg cttgcgccct gctcgataag
    24661 gcgggaggaa acgcttccag aatacgcggt cccccttcga tgagcagaat tcgagaagcc
    24721 gcttggtgca gcaccggaaa atccggcgtc atcgcctttc gcccgatctc cgccagcgcc
    24781 cctgccaact ccacccccgt cgggccgccc cccacgatcg cgaaggtcag ttcatcacgc
    24841 gcctcagggg tgcccttcag gcgctccgcc ttctcaaatg ccatgagcat ccgttcgcga
    24901 atctctaatg catccggcag ggtctttaaa ccaggggcat cctgctccca tgtattgtgg
    24961 ccaaaatacg agtgcctggc gccgggggag agaatcagac tgctgtaaga gaccggctca
    25021 ctattccgga gctcgatgcg acgggcggaa cgatcaacgg cccgcacttc atccatcatg
    25081 atccggacgt tctgttgggc ccgaaggagc gttcgaaggg gccaggcgat gtcagccggt
    25141 gacagggctg ccgtcgcaac ttggtagagc aacggttgga acacgtgatg attcattcgg
    25201 tcgatgagga tgacctcccc cttcaagtgg tgcagcgcgc gcgccgctgc caatcctccg
    25261 aatcccccac cgactatgac gaccggattt cggctctgct ggttcactcg cgcttctcca
    25321 tcacgcttac ggtgaaaggc atcacagcag gcgtctcgct ggacgcatca tctctgcacg
    25381 agcacaacgg agccactagg gagccgccta gttctggaaa ggcacgcgct ctcgctatga
    25441 tccatctcgc aggagtggct cttcatcacc acacattctt gtcggaaaca aacgccagct
    25501 acctgccttt cacatcacac ccaacattca cacacattta ttgtgccggg aaaaggagcc
    25561 aggtatgaag atcgctcagg tcgctccctt atgggaaagt gttccgccgg tgctctacgg
    25621 cgggacggaa cgcatcgtct cattcatcac ggaggagctg gtccgacaag gccacgaggt
    25681 cacgctcttt gccagcggag attccacgac aagagcccga ctgatgccga tgtgtggcga
    25741 agcgctccgc ccctcaaaag tcgtcatgaa ttatgaagcc cctttaacag ccatgatcga
    25801 aaaggtattc gggtcagcac ccgaatatga cctcattcat tcacatcttg aattcattcc
    25861 tttcccgctg gccaaacgat gccgaacgcc gatgctcacg accttgcatg cccgtctcga
    25921 tcagccggag ctcatcccgg tgttccggaa atttgccgac cttccgctgg tctccgtctc
    25981 ggaggcgcaa cgtgaaccgc ttccctgggc gaactggcag gcgacgattc atcccggtat
    26041 accgcattcc ctctatacgc ctcagtacaa ccagggacag tatctcgtgt tcctcgggcg
    26101 cgtcgcgcct gagaagggac tggaccgggc tattgaaatc gcgaagcgcg ttgagatgcc
    26161 gatacgcatc ggtgccaaaa tcgacgcgca gcatgcggaa tactataagt cggtcatcga
    26221 gccgctcttg gaccatccac tggttgagtt tgtgggagaa gtgacggacc ttgaaaagga
    26281 tgatttactg ggagatgcct atgcgctgct cgctccctat gcgtggcctg aaccgttcgg
    26341 cctggccatc atcgaagcgt tggcctgcgg cacaccggtc atcggatcgc ggcgcggcgc
    26401 catccctgaa atcattgaac atggcgtgac cggctttgta tgcgacacac tcgacgagat
    26461 gacccaagcc atcaggcatg tgtcgctgct cgaacgcacc cgatgccggc aggcatttga
    26521 gacgcgattc accgcggaac gaatggccca ggaatatgtg gcagtctacg ggcagatcct
    26581 gggacttccg gacaaggctc ctcaaccttc atccgacctg gcttccgggt tgcctgctgc
    26641 cggccagatc taattccacc ttcacgcacg aagggcaggt actctgttga gcacctgccc
    26701 ttctcgaacc acctacttct tctacttatc gcttactgct gggattgcca ccttcgaatc
    26761 ccggagcttt ccctgaaggc agcccgctgg tgccgctcgg tccgccgcca cccacagact
    26821 cttcacccat ggagccggtt gcgtcaggcc ccttttgtcc agggacgccg gcgctgccag
    26881 gaacggtcgg ggcttcccgc acctgtccca tcttatcttt gtattcttgc aggtcgggag
    26941 acttgtcttt gctgctgctc ataggcagcg cattttgaat ctcctctttt ttcaaggtca
    27001 attggagctt gtcgcctttg gttttaaatt gactccacct gatgggttgt ggcctcttgg
    27061 attcattgga gacgaagacg acatattcaa tgtctccggt cttttcatct ttcatgacct
    27121 gctggatctt gccgatctct tctccgttgg tgccatagac agaggcattg agatatttgc
    27181 tgtctttcat ctcaacgagc ttcccctgtc ggacgggtgt atcagcatat tcttccggga
    27241 taccctgttt cccgcccgtc agatctgttt tcttgtcgaa tgaaggggat tccttgggcg
    27301 tgctggtgcc cgcgccctta cccatggtgg aatctggcgg tggcgcgcct gatccggttg
    27361 agacctgact gaaggccggc gtgccggtca tcaacgccaa cgcaatcgtt cctgcataca
    27421 tggccattcg tcgattcatg tggattctcc tttgtatgag atgtcgtggt ggacgcccct
    27481 actgatttgg ttttaccaat agggcttgta gccgtaatag gtatggagcc gctcggccca
    27541 ggcggtgtcg gccatgtccg gccagcgatc cttatcgaat ccttccgcct gctccaaacg
    27601 ttcccgcggc acattgagaa tgaagaaatc tcctcccggg gagatcgtga gcgcccccca
    27661 cggcagagcg aacagcttgt cccccaatcc taaaaacccg ccgaaggaca gtacggcata
    27721 ggcaatccgt cctttttcga ggtcaatcat gagcgaatga atctcgccca gatcttcgcc
    27781 tgccgcgttt tgaaccgcgg tgccttcgat cgttgacgct gtcaataatc gacgcccgat
    27841 atgtgtggcc atcattttcc tcctctaatg ttaggaatca ctgcatatga gtgttcatgc
    27901 taatggcgct gtgcccttgt ccgtcgtccg tcgaannnnn nnnnnnnnnn nnnnnnnnnn
    27961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28021 nnnnnnnnnn nnnnngtgat tttgaccaat agctgcgcca atgaattgtt catgccgcaa
    28081 cgggccacga acgcctgaaa cgggagaaac tcctcgagga acgggttgga aacagaccgg
    28141 tccaggatag cctgcacaaa tctcttcacg ccctcctcgt aatcttcacg aggattgatc
    28201 cagccggtgt gcaccttcgc ttcgcggatg gctttgatca tgtactgttc aatccgcttt
    28261 ttgaactcct cgtactgagg ttgatccatc ggagtcgagg gccaggcccc gagcagcgtt
    28321 tgatacagca gatactcctc gttccgatcg ggcgcgacag tctcgtccac tttgactcga
    28381 aagcgtctat tatgtttcgc ccagctggcc agggcagctc tccagcgttc cggaatctcc
    28441 gacaggacat tgatccttga gcgaacgtcc tcgcctcgtt tggtgtcatg tgtcgacgta
    28501 gccaacaggg catgaggcca atgcgcacgg cgttcccgca gcgccttgtg aaaggcgtcg
    28561 acagaaagtc catagtgagc cggttcacca ccgacttcat tgagcgacac gaggcggttg
    28621 taaatataga acgcggtgtc ctcgatgcct tttgccgtca cagggctcgt ggtctgttgg
    28681 aacttcgtca caaatcgcac ctgatccggt cctgcgagtt tgatccgatc atccaggcgc
    28741 ttcaggagaa ggtctcgcac aaagtcgaag acctggctgt tcaaggtggg attccggcgt
    28801 ttcgccctgt tcacggccat catgatgtag gtgcggtccc gctccagcac atctttttga
    28861 tccgccgtga tgtaggagcg atagacagga aaacaggcga tcagttccgt gatggcgtgg
    28921 gtcaaactat tcaaagtgaa atcccgatat tgccggtctt tttccgaaag cagattcaat
    28981 tggtgtccca gcacgttcag ctcgctggac atcgaggccc gcatgatcaa ctttttggtg
    29041 agatacacca ggtcgtcgta cacaatccgc tgaccgacga aacgctgata caaggcatcc
    29101 atggaccgtt ccttcgccga ctggacgaag aggccattga cgaggttcag aaagtcatag
    29161 ccggtcgttc cggagaccgg ccaggtcacc ggaagcgatt cgcctttgcc gaggatcttt
    29221 tcgaccacga cgaacaacga ggccggctcc cccgaggcgg ccggctcgag ttctcgggcc
    29281 gcccactcct gcaattggac caagtaatgt tccgggtcat agagcccgtc gacatggtcg
    29341 attcgcaggc ccgtggccac accgctcttt aacagcctga acaccaactg gtgagattcc
    29401 tggaagacgc gtgtttcttc cgtgcggatc gcggccaatt cgttgatatc aaaaaatcgc
    29461 cggtaattga tttcctcgga agccacgcgc caggacgcga gacgataact ctgctcgtcg
    29521 agtaacgcat ccagccggtc gaaactggcc gaatctcctt tcacaccgtt gtacgtatcc
    29581 acggtggccg acagatgcac gcgaatggtc tcactctcct ggagtaacgc tgccaggcgg
    29641 cgcctgatca tgtgcgcttc acgataccgt tcagcgatgc gtttcgggtc acgctctttc
    29701 ctcgccggaa gattgcgtaa ggcggtcaaa atactgagca gttcctgaac gtgctcatcc
    29761 tgctccccca cccgcgccgt caactcttcc aggcggaagg ataggatccc ttcccaggat
    29821 tggggcgcga caggaaaccg gtgctggaaa tacgtgacga tgaagccgcc attctcgtag
    29881 accagccgaa tatcctggtt ctccaatacc gcgccatact gctcgccgag gataggcagc
    29941 agcaccttat cctcgagctc gcgcttcaac ggcgaccaat cgatgtcgaa cgctgtggca
    30001 tagcgagatc ccgggccatg ctcgagaacg tcccaccacc accggttctc cgtggagcgg
    30061 atccccatgt gattgggcac cacgtccaat aagaggccca tatcgtattg tttgagcgcc
    30121 gctgtgaacg cagcgaagtc ctcttcggtt cccagttcag gattgaggca ttcaggatcc
    30181 acgaggtcgt agccatgcat actgcccggc acagccttca agatagacga cacatagcaa
    30241 tcggtaatgc cgagcgcgta gagataggga acaatgcggc cggcgtcacg aaaggtaaac
    30301 gtatggttga gctgaagacg gtaggtcgcg atgggaatgc gcggcatacg tatcagagcg
    30361 cgtgccgccg ttcaatagga aggcagtcgt cgcttctgct cctctgttgc cgcctgaggt
    30421 ttggggatga ccagcactcc cgattccgcc cattctgagg gcgcgtccat ctcctccccg
    30481 cgctcttgcc cgcggggtcc gagatcgaca ttggccggaa tggtattgtg ccgatcgacg
    30541 accacgcgct gcagcttcgc gccggcgcca atgtgcgtgt gatcgaaaat aatagagtct
    30601 tcgatatagg cgccggattc gattcgcacg tggcggccga tgatcgaccg tctgatgtcc
    30661 gcctcgattg cccgtgtgcc ttcgccgatc aaagcgtgat cgacataaca attcacgaga
    30721 cttgccggcg gaccgtagga cggttccgtt ctaatcggcc attcccgatt ccccagatca
    30781 cagggcggtg tatcgccgag caggtccata ttggcttgcc aataggcctg aatggtgccc
    30841 acgtcccgcc aataggccgg ttcctcgtga tacccgacgc ccggcacttc attatcgagg
    30901 aagttgtacg ccatgacccg gtccgttttg agacggcgcg gaagaatgtc gcggccgaag
    30961 tcatgcgatc cgccctcctg cttggcatct tcctccaggg cctggatcaa tacatcggca
    31021 ttgaagaggt aattccccat ggacgaaagg gcatgcgccg ggtcgcccgg catggatttg
    31081 ggcaccgggg gcttttcgtc gaaccccacc accagactcg tgcgatcaat ctccacgatc
    31141 ccgaatccct gtgcggcatg cactggcacc gggagtgtcg cgaccgatac atccgcttcc
    31201 cggtcgagat gaaactgcac catctgccga atatccattc ggtaaatatg gtctgcgccg
    31261 aatacggcga cgatgtcagg agcaaaatca cggatcaggt tgaggttttg aaagaccgca
    31321 tctgcggtgc cttcatacca tcctgctccg gagttcattt gaggagggac gacggtgaca
    31381 aattgccgct ctccatctcc aatccgccat gccttccgga ggtgctcgat cagagattgc
    31441 gatcggtact gcaccatcac gtgcatgccc agaatgccgg agttgtaaaa attgctcaga
    31501 acgaagtcga taatccgata ggcaccgccg aacggaacgg ccggtttgct tcgcgcttcg
    31561 gttaacggca tgaggcgcgt tccttttccc cctgccagga tcatggcaag aacgcgaggc
    31621 tgactgatcc gtccatgttt ctccataacg ctcctcggta ggcaggatga ttgcagcggg
    31681 cacggccact atcaccttac aaaaaatgtc gccgatcgcg tactggacaa atccctagcg
    31741 cgacctctgc acgatccggc agatgatgga cccatgtcag cagtgaagga aatttttcgc
    31801 ggcagaaacg gatcgagttg agctaggttg cgacggcttg gtctcccccc gcgggagcac
    31861 gggaggcgct acaatttgcc aacgagatcg agacctggtg tcagcgtggg ctgcccgggt
    31921 tgccagttgg ccggacaggc ttccccctcg ccttcacgca ctcgaatggc ggcctgaagt
    31981 ttgcgcaagg tttctccgat atatcggccg atactgttgt catgtacttc gtacgcgcgc
    32041 agcacgccat cgggatcgat gaggaaggtt cctcgcaggg ccacaccctc ctcctcaaga
    32101 tagacgccaa actcccggct caacgctccg gtcgagtccg caatcaatgg aaattcaaca
    32161 gtgcgtacca tcggcgaact gtcgtgccag gccttatgca catacacgga atcggtgctg
    32221 acgccgcaca cttccgcacc gagtgtgacg aattcaggat acagtcgcgc cagttcggca
    32281 agctcggtcg ggcacacgaa cgtgaagtct ccagggtaaa acatgaggac caaccatttg
    32341 cctcgcaatt cttccaacac gagagttcgt atctccccct ggtgataggc ttgcacggtc
    32401 gatggcgaaa tttctcgtcc aatagcgatc atgatgtcct ttcctcgaac cgtgtttccg
    32461 gtggtccttc gtgcgggcct acaacgcccg taaaaatgct accagggcag ccttctcatc
    32521 gttgttcaac ttcagccccg ccacgacatt aaagaactcc acgctatcct ccagcgtcag
    32581 gagcctcccg tcgtgaaaat atggcggcga atctttgatc cctcgcaacg cgaaggtctt
    32641 gatgggacca tccgcttcgg cgccgaagcg ctctaagaag agatcgtgca tcatgtgatc
    32701 ggtataggct ggaggttggt gacagactgc gcaacgggct ttgccaaaga acagatcctc
    32761 tcctgcaatt tcctgccggt cggcgcggga tcgatccaac cgtccggtca tgggatccag
    32821 ctttggtgcc ggtggaaagt cgatcatgtt ttggatctgt gccatgtggg ccacttgcaa
    32881 gcgatcgatg atgctgatgc ccttcttggc ggcacgaatg tgatcgccgt tgaaataggc
    32941 tgtacgctgt tcgaattctg taaaatcttc cacagagcgg agactgcgct tggagccatg
    33001 tatctgctga ttgaacattc ctcgcaggct caccgtatcg agacgcagac ggtcacgctg
    33061 cgggcgggta tcgggattca aatgaaattg gcccgtcgta tgaaaattca cgtgacagtc
    33121 taagcaagcg acaccggcgc taggcttggg cgatttgcga tctgcggtca ggttgaattc
    33181 ctcctgcgga aattgcgtga ccagcattct gaggccatcc aattgcacgg gcgtgatcaa
    33241 cccacgaaac aggcgatcaa aattctccac gctcaagact tcgccctgcg acacatcacc
    33301 taattcagga tgggtggtaa gaaaaatcgg cggcggaaac tccggcagaa agcaagcagg
    33361 cagatctaaa tcgacatcga accgctccag cctagggaac tgatggatct gttcctcggg
    33421 aaacaccatg cctcccacct cgtgcttcac gtgaggcaat cgcatgaaac cggcgggaaa
    33481 cgccttttgc tttcggattt cttccggttc catctgggac agggattccc acgtgaggcc
    33541 attcttgagt tggacggtgg ggccaatcgg ttgtggcttt ccttttgtca tgcgtacggt
    33601 ggaatgtgtt ttacactcca gtcgataacg gtcctgtaag agtctcgcct gttgggccat
    33661 gaccttgggt ttctcttcct gatccttctt catggcttcc tgagccaggc gagcggatga
    33721 ttgcccggga ggtccgccaa agaggtcgta cgagaaatcc acaccttcga atcccttttc
    33781 cttggtcttc tcaatggccg gattcactgt gtgccgcagt tcttttccgt cccgatcggc
    33841 atagacatat gccacagaca tagccagcac cactccaacc gacaccaggt gcgatacagc
    33901 ttttcccgcc gtccgtgttg tttttcgtct cacagtgtca cctcgtcata ggggccaaat
    33961 cgccgcatac gctcgcggtt ctgtttgaaa caatctgcac aggaccggaa cggttgtcct
    34021 actaggcaga cccctgggcc ggagccgttc ttccctatga ctcagtgaaa ccagggagtc
    34081 tcctagtgct ccgccggtca tctgatttct aggatgggag tcgataccaa acagaacaga
    34141 tcttgcgtgg aaaggaggcc tactatgaat tgtcatcgat gcagcaactt gatgtttccc
    34201 gttgcgcttc aggattgggg cggaggactc atgaatcagg atgtcgcggc ctggcgctgt
    34261 ttcgcctgcg gagaaattgt ggatcagctg attgcgcaga atcgtacccg cggtggcgaa
    34321 gatgcggaag agtggcgccg agggggcgca cgccggcggg tcaacggaat cggactctta
    34381 cgttagacga caatacctcc catttattcc gggcgccggt gagcaacatc ggcgccctgc
    34441 ttatccttcc tctcccgccg gctcatccag gcgcagtcaa tcatggacta ttcggggtgg
    34501 gatcatagcc ctcaaacggc tgccgtccca ccaccagcac caccgcaacc agcgctagag
    34561 cgatgagcag ataaaacccg aagcgaaacg cttcggtata gggtgtgctg aacgttccga
    34621 acgcatagag acctgtgaga atcatgccta tcagcacgac ggcaaccagt agggtcgtca
    34681 ttgcagcacc tccagcgtaa gtggtgagtt tcccctcagc cgacgcttgc atcggcggat
    34741 catcaaccgg tggatcttgg tgcggtggag gatggtccgg tggttctttc tccggaggat
    34801 gccgtggccc tggttccttc accggcggtt ttgacccggt ctgcgtcata gggtcacttc
    34861 cagcaatcag caacacccat gcccccatgc ttctccctga gcatgagcga tcattgcaga
    34921 tttccttatc accccgagcc cggtaccccc gcaatcgcgg atggcctgtc ctccaaagga
    34981 cagattgtct cccccggcca ttgcagacac accttattgc tgacacgcaa aacaccagaa
    35041 acctcgcctc cactgcattt tttgaccagc gtccgtttca agcacggcaa cggtccgtct
    35101 tttgctaact acggcatcat cggccgacct gcagtgaacg acatacgaag gagtcaccat
    35161 gaccgtacac atgaatcgct ttctgccaca tcagggcctt catttggcaa ccgctgtcgg
    35221 tatttccatg atgctcagcg gggtaccggt tgccatgacg tccgcccatg ccgccgagga
    35281 aaccaaaaca ggtgatacct cacagcttcg tgaggatgca caaacgttac tcaaaggaaa
    35341 tcgcactgtg ttggcaacag tacttgccgt cacctcggat cagatcaaag tcgacatcgg
    35401 tgaagttcaa ccgcgttttc tcccgctgaa gcaggcacaa cagaaagggt tcccgaaagt
    35461 atccgaaggc gatgacctca tcgtcgtcct gaacgaacaa aatcttctgg tcgactatca
    35521 tcctctagac ggaggcacga gcgcccacac catcatccgt ggggaacttt cgcagactct
    35581 tccgaccggg catgaaacgg ctgtcataaa gtcggggggc aaagaacaat cgttcaaaat
    35641 ccgctctcag gcgcggagta aagtcgcggc catcccccgc ggagccacgg cggtttttct
    35701 gatcgacgag acgaatcaga ttgccgacgt cagcctgtcc cgccaacagg cggcccaact
    35761 cgcgggagcg cattctcaac cgattaaagg ccagcatcaa caggttgaag gaaccgtcac
    35821 gtctcccttg cagtcgaatc acattacgat tcgcactccc ggcggcacgg ataagccata
    35881 cgaagttaga gacatcctcc agtcaaaaat cgcgacactc cacaaaggag atgcggtcgt
    35941 gctgctgatc gatggcgaca ataaagtcat tgacgtggcc attccgccca aagggaaata
    36001 aagcgcacgg cgctgtggtc tgggacaagt ccgtcacgcg actccataac cggctgcgag
    36061 gccgtaatgg cgaagtttcc gcagcagtgt tttccgatga ataccaagaa tcgtggcgga
    36121 tcgtcccaga tcccgcgcat gggaatccaa cacttgaaga atgtggtttc gctcgagatc
    36181 ttcgagggtg acccaccctt gcgcgcgtgc caacccttca ggctgcgcgt ccttgaccgc
    36241 cggaggaagg tcttcggggc agatgacgga ataggggctc aacgtgacag ctcgctcgat
    36301 cgcgtgttcc agttcccgga cgttgccggg ccaccagtag cggcggagca gggccagcgc
    36361 ctcagcggac aattccgtga cgggtggcat tttcatcgac ccgtacaggc gcaaaaagcg
    36421 tttggtcaac aatggtaggt cgtcaagacg ctctcgcaat ggcgggatgt cgatcgtcac
    36481 gacattcatt cggtaataga gatcctctcg gaatgtgccc gcccgtacga gaggctccag
    36541 cggaagctta gaggccgcaa tgattcggac atctaacgcc gtcgttgcgg tacttccgac
    36601 acggcgaatt tccttctcct ggaggacacg cagcaacttg ccttgtaatg ccggcgacag
    36661 gtcggccacc tcgtcgagga aacaggttcc ggaatgggct tgctccagca gcccctttcg
    36721 ggaagagatc gccccggtga aggagccacg ttcgtgcccg aacagctctg actccagcaa
    36781 gctgtcggcc aacgctcctc catcaacggt gacgaacggc ccggagcttc gaggtccgct
    36841 cgcatggatg gcccgggcga tcagttcctt gcctgtgccg ctttcgccct gtagcaacac
    36901 ggagctgtcc gtttgagcca cccgggcgat cgctttgtag acctgcacca tggtggggct
    36961 gctaccaacg aggctggcga agggggacgt gtcgtcagga gcggcttcgc ccgatgcagc
    37021 ctgctcggcc cgcatatgag tgtgttcgac cgcacgtcgg gcgacagaac ggacttcctc
    37081 ggccgtgaac ggtttactca tatagtcaaa ggcgccggct ttgagtcctt ctaccgctgt
    37141 ccgtagcgaa ccatgagcgg tcatgagcac gatcggggtg gtgggacaga gtccgcgcaa
    37201 ttgacgcagg caggtcagct cctccgtttc aggaggaagg tcagtgagaa tgagatcgac
    37261 tgggatccgt tcgaggtagc cgccggcgtc cttcgggtcg cgaacggcgg aaacctcata
    37321 tccgtcgacg gacaacatat ctcgtaaatc agtcgcagcg cctacgttgc tatcgacgat
    37381 gagaatacgg gcctgtgtca tggtggttat tatccatagc gaggtgatgg tccaatgcac
    37441 atgagagtca tagcctacgc tcgcggacaa tcgcccgaca cctggatgaa accctggtct
    37501 ttgggtgtct ttatgatgcg cggcaacgct cgttctgacg ccgaacacac cttctctacc
    37561 cccgctcagc cccatcagga gaagatgtgt gcacttgctc gcacagtacc atggtccatc
    37621 ctcctaaccg tcctctgctg tatggtgatg ttttctatgg tgatagtccc ccttctctgt
    37681 ctttgcccag ccgttccaaa agccagaacc cattccgccg gctcccatcc ccggcccttc
    37741 ccctaaaccg ctgccgcccg cgccaatccc tgctcccgcg ccgaagccca ttccccccgc
    37801 tccaccgagg tgacgcacag ttccctttcc tagagagtcc agcgtcttgc gcgacagaaa
    37861 cagggggttc tcctcggacc gatggagtaa cccgccgtga tacgcatgac tagagactca
    37921 ctccttccac cgttcatgaa gggagcggtc atgaagagat ggctggcttg ctgcgcatca
    37981 gtgatcgtga cggctatctg gttcccctgt tcgtccgggg cactcgagtt cacggccgat
    38041 cgggtgacac acaccgatcg cggatttcac cgagcgcgga ttttctatca tgacggcatg
    38101 tggcggctgg agcacaacga gcgagggccg gtgagcatca cgatcgtcag actggatcga
    38161 aatcaggtgt ggcatctgtt gccggtgacc catcattaca aaaccgtctc gtacgatcgc
    38221 gattatgccc ttcacctggc gacgacgctt gatcatgaaa tctcgcggga aacgatcggc
    38281 gtccagatct tagacggcca tccgactgtc ttgtatgagg tcctgaccat cacgccggga
    38341 ggcggaagag aaagttatta ccaatggatt gcgacagaca tcaatttccc gctgaaactg
    38401 gccaagaaaa atggcgactg gatggtggag tatcgtcacg tcaagttcgg gcctatctcg
    38461 gacgtgtttt ttgagctgcc acgccactac gcgcctatgg acgacgatca gcacaccgcg
    38521 agttcgccgg agccagtaag gaggatgagc ccatgacgac aaggacctcc cgacatcaga
    38581 tgaccggggc aagagaccat atcgtgatcg gactgctgcc gaacatgggc gcggtgcgag
    38641 aagcgctgga agctctgcgg gctcgtggca tctccgatga tcagatcggg ctcgcgatgc
    38701 gccaagctga agaacggtta tccgacgagg agagtccctc gcctacagca caggacgcga
    38761 cgaccgggat ggtcggcgga ggcattatcg gcggactggc aggtctgttg accacgaccg
    38821 gcgtggttgc cattcccggt ctggcaccat tgctggccgg cggctcactc atcgggctgc
    38881 tgggcgcaac gggcgcatcc atcgtggcag ctagcggact cggcgcagtc gccggaggac
    38941 tcgttggcgc gctgatcagt atcaacgtgc ctgaaacggc cgccaaacat tatgcggatg
    39001 cggtccacga gggacagatt ctcatcagcg tgaagaccga cggcgaccct gcccaggttg
    39061 aagagctcct cgcttctttg ggagccacca cggaccggca atcgccctag cgctggtcgc
    39121 tgatctttct agggaaacca ccagcttcgc cgtcatggga cttcgaccat gatgtcctca
    39181 gcacgcactg aggaaacctt tccaactttt acagggagca ctgcccaatc ggcactatgg
    39241 ctaagcaaca agcatcccgt acgaagagca acacacctgc tgcgcagaaa gttgtctctc
    39301 agaacggatc ggccgagcca ccggttccac accacgaacc catcaaaacg ctcgtcgtcg
    39361 atatcggggg cacaggcatt aaatccatga tcgtcaatga agtcggagag cccattgggg
    39421 aacggttgcg gctcccgact ccgaaacgaa aaactccgcc cagcgtgctg aagaccattc
    39481 atcgtctcgc caaactgtcc gggcgtttcc atcgggtcag cgtcggattc cccggcacca
    39541 tcagaaacgg cgtcatcctt gacgcggtga acctcgaccc ttcctggaag cacttcaatc
    39601 tcatcgccgc gctcggcaaa accctgcgca agaagcccgt tcgagcggcc aacgatgctg
    39661 acgtgcaggg gtatggcgcg atttccggaa agggagttga gctggtttta actttgggaa
    39721 cgggcgtcgg gtcctccctg ttcatcgatg gccgcctcgt cccgaatttg gaagtcggaa
    39781 agaatcggct gcgaggctcc gagctcgaac gcatcggcaa aaaattgtgg aacaatcgct
    39841 tgctgaaggt catcgccaag cttgaaaaaa tgtttcacta cgaccgtctc tacatcggtg
    39901 gtggcaatgc cgtgcaggtg gatatcgggc gggtccctgg caatgtcacc gtcgtctcga
    39961 acctgaatgg actgctgggt ggaatcgcct tgtgggaaga agccgcccac ttcgctgcga
    40021 gcagacagga actggcgaat aacaacgcca accattctgc cccgcctaag ccggcagctg
    40081 aacgcgccgc accgcgtggg cgctgagcag attgctccag cgagccccgc gactgagaca
    40141 agttgtcctt gctcagacaa tcggtccgta ccctgtatcg acctgcgttg ggcatcagtc
    40201 gtgaagatct ttcaaacagc gctgccagaa agcacagtcc acacgtttca taagcccatg
    40261 agaagaccct gctgtggtcc cgcatttgca ccggtgttgt gttgaagaat gtcatacagg
    40321 catccgtctg gtctcttaac aagaatgatt tggaggatca catggcacac atgcgcgggg
    40381 gagtcagaac agcgccacct gagcgccgtc acaagtcaga gcaggacagg aacggcggcg
    40441 gccggatgga ggcaaggccg catggccgac aagatgagac ggaccagaac gtgggtgaca
    40501 aggaacggtt gatctccggc ctcgtcgggg gcggcctgct gctacacagc ctgagaggtc
    40561 cgttttccgg cctgagcgcg ctcaaagccc tcctcggcct cgcactcgtg cagcgctccg
    40621 tcaccggtca ctgtgccggg tatcacatgc tcgggatgaa cacgcatgac cagacggata
    40681 catcatcgat aggacgctcc aaagtgcata gcgaccgggc cattaaaatt gagcgctcca
    40741 tcacgatcca acgatctgcc aaggagctct acgccttctg gcggcaactc gacaatcttc
    40801 ccaccatcat gagtcaggtc cgcacggtcg aacctctgga tgatcgcagg tcacattggg
    40861 tcatcaacac cctgcccggc gctcctaccg tggaatggga tgcggaaatc atcaatgagg
    40921 tcgaaaacga gcgcatcgga tggaaaacgc tcgatggcgc aacggtcgaa cacgcggggt
    40981 cggttcggtt caatccgttg aacgatgaac gccagacccg ggtaactgtc acattgcaat
    41041 atgatccgcc tgccggggcc atcggtgccg ccatcgcaga tctgtttgga caggagccag
    41101 ggcagaagat cgcccgcgat ctcgagcgtt tcaaagaagc gatggagtca cacaccaccg
    41161 tctctcagtg agcagacagg agtgcgcggt tcgattccta ttgcgcatgc gttcggtctg
    41221 ccggagtgaa agggggaagt atgaatatgt ggcgaggcct cacggtcgca gtgatggtga
    41281 tgggagcgac tgtcatttct ggagtgccgg gtggagccgc tcaaaaggaa tccatggaga
    41341 agacgccgaa ggtggaagac gacacatcgg acaaatctgc ggggaaggcg tccaaagata
    41401 aggtcatcaa gaaacggcgg tcatcaggca agagcaatgc cgaagacaca accgatgcca
    41461 aatcacaacc caagtctcaa gagtcgcgtg aggggacacc cggcacgacc ttgatgttca
    41521 ggtccgacgg cgcaggaaac tccggcgcag cgggcaaata gacaggatca gcggagaact
    41581 tttgcatcca taatcttgaa ggaggcacac catgccgaaa cagaacaggc acatccggct
    41641 gttcgccgcc atgctcggac taggaatcat gagcgccaca ggggtctatg cggaccaggc
    41701 acgtgaacag agagaagcgg gcctgcaggg gcagagcgat gggcgagtcc acaatgcaga
    41761 tatcaccggc ggcttctatg acgaccgcta caaactcgac gactggtact acgattttta
    41821 tgacagtccg ggatattccc gtgctgattc cacgtccgtc atccaagcct ccaacgagaa
    41881 tacggccacc atggcagatg ccgctcatcc atccttgcgg catcatcggc cctaccgcac
    41941 tgaccagaat gtcccggcca cgccctatgc cggagatccc tggttctatg cacagcggga
    42001 ccccatgtac ggcataccgg aaaccagctc ggatgcgtac ggtggaaacg tcatcaaagg
    42061 cacgatcaag gcggccaaac aagtccgcaa ccggacaacc ggtgatcaaa ataccgtggt
    42121 attgctcacc actccggatc gccggcaagt caccgctgac cttgggtctt cacgacgcac
    42181 catggatctc gcactgacga aaggtgattc gctcgtcgtg gccggtccat gggaggatgt
    42241 gggtccttac tccgtgctta tggcgcagca gatcaggacc gaagccaagc gcgtcaccct
    42301 gaaccgtccg atcggggccg cccgcggaga caggaccgtg gaaggtcgta ttcagcaatt
    42361 tcgagacatt cgaatgaatc gcagcgggga actccatcgc gcagcggctg tgcaaacccc
    42421 ggatgggcgg atggcacttg tggaccttgg gccggataat caggaccgga cacccttgcg
    42481 agctgccgcc ggcgaccgga tggtggccca ggggccggtg gttcaggtgg gcaactaccc
    42541 tgtcctctgg gccaaccgcg tggccatcaa tgacggcatg ccggttgacg tgacgcgtac
    42601 gactgcgccg actgacttcg taccggcaac cgggaacagg caggaaaact gtctcggtgc
    42661 cggctgcgac aaccagccgt taaatcaggg ccagcccggc aaccggaaca gcaacgcgat
    42721 ggatggcacc actcgctgat ccgggggact ccctcctgcg cagggaaggg ggacccattg
    42781 gatcctcctt ccccattttc acgccgctcg tgctgctggt gtcgatcatc agataggggc
    42841 caggcccggc cctcgggtct cgaaacgttg tggggtacgg ggggatgtgc cgatgcgggg
    42901 cgcgcacggc gagttgtcgt tgttgattaa caacaagggt gtcgcgacca cactgtttac
    42961 aaacgaatcg tgatgctgct tcgcccggaa acggggtcca agctcgtcac catctctctc
    43021 accttgcgct ccaccaccgg ctcgattcgc tcacgaagaa tccgttcaca ggaaagaaac
    43081 atcgcctcgt gaacctgccg taacaatgag cgtccttccg gtgattcggc caatcgcctc
    43141 tcagccggta ccgatccctg cctcgtcaac gtcacttcga tttcatcctg agacagatgc
    43201 acctggacat tggcatagtt ggatcgcatg aactcagtgt gaaagttcag gatcgtcatc
    43261 atgagcgcat actcgagatc ggctttattg gtgcttcttt gtgccatgct ctgctccccg
    43321 atagtctgaa gacgaatcgc aacctgccga tgccgcttac gtgcggaggc tgtcaaaccg
    43381 gacagtgacc actcgcttca cactgtacca tgaaatcgca tcccggccga tgcacggtga
    43441 aacgacacgc gtgggacatg ccggcctatg aagtcgccag acatgatgaa gcctcggggc
    43501 acatggggcc ccgaggcttc cgtgaggcta ataggccttg ctgaaggccg gttcaccatc
    43561 atgagtccag agcttttgcg ctgcggacca ccaccagccc ttcttagtca agaccgccag
    43621 gaatgtatcg acgtcagcat cggtcacatc ccctgtcgcc atgaaccgac aacgatacca
    43681 gttcaccatc atcagatcgg gactgacgaa ccccggccag accttggttc cccgattgga
    43741 gatgaactcg catttaaacg gcccgaactc gccgtccgga aatttcggaa tgccttcttc
    43801 ggtgacgatg tacatgtcca caccgacgag cttgataact ttcttcttcc gcgtcgcttt
    43861 cgctgcttca gaaaacgttg ttgccatggg cgcttcccct ttctttatca atggcgggat
    43921 tctcaggctt tcccctgcat cttgtcgacg atggcttgag catattcgtc ggtggtggcg
    43981 gttccgccta cgtcgtaggt gacatgtttg ccttcatgga ggaccgcgaa catggctttt
    44041 tcgatacgat cagccgccgc agtctccttc aaccagcgca gcatcatgat gccggacacc
    44101 aacaccgccg aagggttcac ctttttcatc cccgcatact tcggcgctga cccgtgcacc
    44161 gcctcgaaga tcgaacagtt gtccccgata ttggcgcccg gcgcgaaacc caaccctccc
    44221 accagccccg cgcagagatc cgtcagaata tccccgtaca agtttggcag taccagacag
    44281 tcgaactgag cgggattcct cacgagctgc atgcagcagt tgtctacgat gacgtccccg
    44341 gtttcgattt caggatactt cttcgcggcc tcgcgaaacg catccagaaa cagcccgtcc
    44401 gtcattttca taatgttggc tttgtgcaca cagtacacct tcttccgacc attggctctc
    44461 gcccactgaa acgcaaagtc ggcaatgcgg taagagcctg gccaggtgat gaccttcagg
    44521 cattgcgcca cttcgtcgga caccatatgc tcgatggccg cataggaatc ttccgtgttt
    44581 tcccggaagt taatgatgtc gatcttgtcc caggggcgct tcaacaccgg aatcagctta
    44641 gccggccgga cattggcaaa caggtcgaac accttacgca acgtcacgtt cgcgcttttg
    44701 tgccccgtcc cgacgggcgt cgtcgtcggg cctttaagcg ccaccttgtt tttggcaatg
    44761 ttcttgatgg tcttatccgg aagcaaggtg ccgtacttct ccaaacatcc caatccgatc
    44821 tcttcgtatt cccatttgat atcgacaccg ctcgcgtcaa tcaccagtcg caccgcttcg
    44881 cagatttcag ggccggtccc ttctcccgga agcatcgtga ctacatgttg tgccatcaga
    44941 gaacctcctt gcgtcaccag ggggtaaccg gaccatccgt tcccccgctc gcacagacat
    45001 catgtgcaaa ggcgattcca cggcgtagtc ttgcccgtgc ggcggtgtct gccgcatttt
    45061 tcagtacaat tcccgtctcc ggcgtccgtg caggtcggtc ccttggtatg ggaacggtgc
    45121 gatcgtgcac agacacacac tgtacgctgt gtgcctgtgc atctgtgctc agcaggataa
    45181 gcggacacta cggttggaca agtgcctggg cgggaaacgt ttttgccaga ggccggcagt
    45241 aaccggccag ttggggagat ccagtcacgg ccaccacccc atggggcccg gcagcctgga
    45301 ccgcacgcct gaatgcggct tccgtactgt ctaccacctc agcggcgacc gaagagcagg
    45361 ttcgccattg ttcggcaatc gcgtcgggcg gcacggccct atcttgtgga ccgccggtca
    45421 cgatgagccg gtcggctccc gctccggcaa gagcgcgcaa cacctgttcc cattgtgcat
    45481 cccgcgccag ggatacgagc aagacactgc ggcggccctc gaaggtcgtg cggagaatag
    45541 tcgcgagccg cattgccgcc ggggcgttgt gcgcgccatc cagaagcacc gcgggcgtgc
    45601 cggacagcac ttcacagcag gctggccatt ccgtcttctc gaatacccgg ccgatctcgt
    45661 ccggatcgat gtccggaagc agtcctcgct tgcgtaacac gtcataagcc atgagggcat
    45721 gagcggcatt ggcggccatg aatgccgcag gcgaccgtag tcgcagctgt ggatgcgtgg
    45781 acgcgccgac ccttaccgta caaagtcccg catagccgtc acggatgaac tcggtgactt
    45841 cataatcctg cccgctgagt agcacgggtg ccttgagcgc cgcggctcga gacaggacca
    45901 cggcccgcgg cccctccggc atcgggccgc acagcaccgg cgtaaagggt ttgatgatgc
    45961 ccgccttttg gccggcaatc tgctcgagcg tgtccccgag catgtctgcg tggtcatatt
    46021 cgacggtcgt aatgaccgcg agctcaggcg cgatgacgtt ggtggcatcc tgccgcccgc
    46081 cgatgccgac ctcgatcacc gcatagtcca ctttggccgc cctgaacgcg aaaaaggccg
    46141 ccgcggtcag gaattcgaac ggagccagtt gaaggccgtg cacgtccaga aacgccttga
    46201 cgtggttaca ggcatcgacc agctcatctt cgccgatggg ctcaccgttg atcgcaatgc
    46261 gttcccggaa attcgtgaca tgcgggctcg tatagacgcc caccgtcaat ccggtcgccc
    46321 gtatcagcgc accgatcatg cggctgaccg tcgtctttcc attcgtgccg gtcacgtgga
    46381 tcgagcgata ggccaaatcc ggccgcttga gttcatgcag gcagcgagcg accgctccca
    46441 ggctgacggt gccgtcggac ggctgccccc gcaggatgca atcgacttca acgcctcgca
    46501 aaaattcggt cacttcggca taggacgaga acggcacggt ccaagacgga ggacaggtct
    46561 tcgttcgtct accccgcata cacacctccc tggctgggga cagttcaatg ccgctgatca
    46621 ggcctgcggg actggcgctt cacccacctg ttcagccata tccgcgacga caaaccattt
    46681 gccgccatca taacggtaat atttggcctc ggccggctcg caggcgatga gtagcagcca
    46741 ccggttgttg aacagtttct ccaggagcgg ctgccgttcg atgatggcgc tgaggcgttc
    46801 cctcggcgcc tcgatgatgg ccgtgaggcg catcggctcg tggtaagggg cctctccaca
    46861 catgaccgtc tgagtcggca agcccatccg aaggtcgctc tgactgcccg tcatcacgcc
    46921 gatcccgccg gtcacgttat gataaatctt gctcccgctg ccgaagacct cgggggatac
    46981 ggcggaaaaa tagtattcca tattgatcca ctgcgcgacg atcaagggcg cggtcatgat
    47041 gatttcgagc agcttgccgt caccatccat ggcatggtcg tatgagtgca ggaacgagcg
    47101 gccgttcaga tcggcctccc gcgtcaatcg cctgctgcct acgataaaga cactattgcg
    47161 cgacaagccc cattcgggac gcacctgcgc ccaatccacg ctgcgccgtt cgagttcctg
    47221 ccgagccgcg cgcgttccct catgcgattc ggcggccgtc aaggccaccc agcgttcggc
    47281 cgccgcttcc ccgccggcct catgaacatc gtccaggatt tgggcaagtt cctttcgatg
    47341 agttgagggg acatcttcga gatcggcgac ctgaacccgg tcggtcgtcg tatcgtggag
    47401 ggcggcgaca aactgtgtat ccggagggat cgtcaaccct ctcgaggcca aaatctcgcg
    47461 aacctgcggg cgattggcca tcatggcaaa tgcgcgcgcg ttgggaagtc cctgtcctcc
    47521 accgcaagcc ccgcaatcca aagacgactc atacggatta ttttgcgagg tgctgccgtg
    47581 accgcatagc acgaccaatc gagcgaagga tccggtgagg cccatcagtc gcagcgatgt
    47641 ctcgacgtag tacgcctgct cattgacggt aaacccggtc cgggtaatgc gatcgagtcg
    47701 ggagcgagtc cctcgatacg tcagccggca ttcgcggcgc agcgtgacca gtgcatccgt
    47761 ttcctctgtg gagcagacac ccaagagccg gccgagggcc ccgggatgcg aggcggattc
    47821 ctcatcttcc tcgagtgctt gacgccggac ctcatccaac acagctcctg tgaccaggct
    47881 tccagcccgt tggccatgtt tccttaacca ccaggcaatc tgcagacgtt gttcggcggc
    47941 aagcatgttc tccgcgtcgg cggtcgaaaa cttatcgacc gtcaaggtgg tggcgaccgg
    48001 cggcacgacc caggatttga cccaggcggc aaatcgatgg taccagcggg ggcagagcgt
    48061 tttcccgagg aagggcaaca ggaaaaacca gccgagtgcc tcgaccatga cgtagggcgt
    48121 gacgacgttg tgcttcaagt cgtggagcag ttcgtggcct gccttgacga gatggctgct
    48181 gtgcttgcgg cgttcggcca ttgcgtcctg atacgtcctc ggcacctccc tgatgaggtg
    48241 tttcggtttc aacaggaccg ggcagagcgg cagttcccgc tgctcgtcca acgaacgata
    48301 ggagaacggc acgccaaaga accccgcgaa tccgtacgtt tcataccccc cgcgattctc
    48361 gagatgcctc cgaaacacct cggaccgtac atcgatgcag aacacgaact gggcgactgg
    48421 tcgcgccgtc atcgcgcccg gcgcggcgat ccgcgcgtcc tcacctgctg gcagctcgag
    48481 tttgcgcagc acctcgcatt gatgactgcg ttcgaatgct tccagccatg ccacacgctg
    48541 tttagccgga gtaaactccc gcatccatcg taacaacata gcgaggtcgc ctggcgaagt
    48601 agacgtgatc tcgtggaccg gcacggcgag cgcgcccgac aggctcacga gattctgggc
    48661 ttccaccctg taggttcgct caccggcagg ctgttcctca aaccaccgcc ggcaatcctg
    48721 ttcccaggcg gctggatcgt atgaccggcc cgctcgctgg aggcgggagg cttcaggcag
    48781 caaagctgaa ggaagatccc cggacatcga agctctcctg aaccagagcg tgtatggata
    48841 ccgtttccca tactccagca ctgcgctggc cgttccctcg caccccagcg aggcacggca
    48901 cgcggtcgcg acaagctcgc gttcataaaa cagccggacg gcaagatact tcacaagatc
    48961 gatgcgatag accgactgcc agacgtgcgc atcctggtct tcccgccact tgataaatcc
    49021 cgcccagcct ggcaaggcgg ccaggtggcg cgacaaatac gattgccaga ttgcgtgggg
    49081 gatgtggagc atctccagcg actccaacag gacgtcttca gggcggtcgg ccagcgccgc
    49141 aattttcgat gccgcccgtt ccacccccgc acacctgaga ctgaaatcgt atcgcgcggc
    49201 agctttccat gcgcggaaaa atgtctcttc ccgcttcggc atgggccacg ccgcctcccc
    49261 ttcgtcgcaa aacgccgcgc accacttgat catctggcgg ttgatttcat ccgtgatggt
    49321 gacgccgagc gttcgatcgc accagtcggc catcgtgctt tcacaaggcc attccccggg
    49381 gatgtactgc aaacccgccg ccctctccgt ctctagtgtc tcgcggggaa gcatgcgccc
    49441 caaccacgcc gccaggtgag ggacggtcac ggagtctcct gtgggcgtag gcggggccgt
    49501 cgctgctgtg cctacccgat cgtggtcgcg gcccactgcc accatgatgg ctctgaggac
    49561 atccaggtga gtaacctggc gatcgccaaa cgtgatctgc cgatcgcccg cctgccctcg
    49621 caaggcctct tcgaaatgtt tctcttgaat gcggcctcga cgaaatgctt cccggaaggc
    49681 ttcccccgag agggtgcccg atccgccgaa cagctgctca gcccgttcga ccgcctcatc
    49741 gaaacggaga tgctcgaatc cctgcagggg gtttcgggcg atcactgacg tcatcggcca
    49801 caccttggcg atggtctctc cggcgaggct cacataagac cgcaactcca ttcgttgtgc
    49861 gtcgcgatga cgctggtccg aaccattcgt tgtatgcatg ccgtccctca ctttgtccct
    49921 gtgtcacagc aagaacgggg ccaaccgtct cccgcaccac cacagatgcg gctggctctg
    49981 atttgttcgg aaggtcgcga ttcaatatct gcctcgcgac gcggcggaag acggtcggca
    50041 aggtcgaggc gtgcaagggt acacgaacgt gcaaccatgc acgtccgtgc agagcgcaac
    50101 ccgttgaaca gggaacgtgg gccatcaatg cgggtgcata ggaggagcta catatggccg
    50161 agcttccgga gggtttccat gccgcgtcct tcgtcttgtg cgcggccggc tcgttcggct
    50221 gtcgtcgtgc ggcgcagttc ggtgatgatt tgaggtatgg tggaggcgca ggaatcaact
    50281 gtgccggtgc aggggacaca acctaagctc cgatatcgtg tgccggctcc cctgtcgaaa
    50341 tatgggctcg gcagctcaat tcgttcctgt tcgagatatt cccagatgtt cagctcggtc
    50401 caatcgagaa gaggatgcac gcgaatgtgg gaaccgggcg gaaacttcgt ctgaaattgt
    50461 tcccagagct ccggcggctg gtcgcgaaaa ttccactcgc cctccttgcc ccgcaaagag
    50521 aagtatcgtt ccttggcacg cgtgccctcc tcgtccgctc gaatgccgag gataatggcc
    50581 gaccagccgt gctgggcgat ggtctggtta agggcatcga ttttcatggc cgtgcagcac
    50641 acaacccggc cgcggtccgg tcccatgccg tcggccagcg ccgccttgtt ctggcccacg
    50701 atgagattca acctccagtc gcggcatatg cgatcccgat attcgatcag ttccggcatt
    50761 tcgtaccccg tatcgatgtg caccagcgga aaaggcacgt ggccgaaaaa tgccttgcgg
    50821 gccagccata acagcaccgt cgaatccttc cccatcgacc ataacatcgc caagccgtca
    50881 aaatgcttat aggcttcgcg caagatataa acgctctgat cttcaagctc tcgcaggtgt
    50941 cgcatagcat ctcccaggca ctagatgaat tccatccgcg caggttttcc agtcgttagc
    51001 ctcacgaccc gctcgtggcc ggatcgacta tcaaaccgtc cttgcaatat gaggtcccgt
    51061 taacaacgac cacccctggc ttcctgcggg agggccatat gccggcaggg gacggcagag
    51121 acactgtccg gcaacgaacg ggtctggaac ggatgagtgg atagatgcgc tggttgtgcg
    51181 cggacagacc ttcccgcgca caggtgtgtg cctgcgcacc ggggcgcagc gcgccgtcgc
    51241 agtcttgtgc ggaagacagg tggggggtgg gaatctggac tgctagtatg ccttgcggcg
    51301 ccgatcgagc atccagcgcg tgatccccag gtgctttgcc gccagggttt tgttgccgtc
    51361 gcttcgttcg aggacggcct gaatgatggc atcctcgagg tcgctcagcg accgctcgcc
    51421 gaccttgaaa gacaggctga ccatgagcgg gtcggcttca gcaggggccg ccgtaggctg
    51481 aacggctccg gggagtccgg ggagtgaggc gggcgccacc gccgcagcca ggtccgccgg
    51541 aaaatgagcc gcatcgagtg acggaccatt gcaaaagatc accgcccgtt cgatgagatt
    51601 gcccagctcc cggacgttcc cggggaagtg ataggcttgc agtaattgtc tcgcagactc
    51661 ggtcagttcg cgaaccggct tcttgagggc cagcgccgac cggagaaggc agtgttcggc
    51721 cagcggaatg atgtcttccg gccgcgctcg caagggcgga accgtgagca tgacgacgtt
    51781 cagccggaaa tagagatctt ctcggaattc tcccttcgcg acagccgcct tcagatcgcg
    51841 gttcgtcgcc gccatgaacc gcacatcgac cggcacggta ttcattcctc cgacccgtcg
    51901 cagcgaccgc tcctcgatca cccgcaacag cttggcttgc agcgccaagg gaagatctcc
    51961 gatctcgtca aaaaagaccg tcccgccttc agccatctcg atcagcccgc atttacggcc
    52021 tgcggcgccc gtgaatgcgc ccttctcatg gccgaagagt tcgctctcga agaggtctcg
    52081 tggaatcgac gggcagtcga cctcgacgca gggcttgctg ccacgcgcac tgttgtagtg
    52141 gatcacccgg cccatgtact gcttgccggt tcccgtttca ccttgcaaca gcaccgtgac
    52201 gcggtcgttg cgcacgagtt cctggacctg ggccagaaaa cttttggtga ccggactctt
    52261 ggcgatcacg cgatccaacg cgtaccgggc ggcatcctga ccgctatgca actgcacctg
    52321 acgctgaagg gtcaagaaat cggtcaccct gcgcagggtg tggagcagat cgtccatatc
    52381 aatggacttg gccacgaaat cgaacgcgcc gagccgcatg gcttccaccg catctttgac
    52441 cgttcctctg gccgtcagca cgatgaccgg cagagcgggc gacatctgct tcacccgcgc
    52501 aagcacatcg aggccggata gattcggcat caccaaatcg actatgagga catccggctc
    52561 gaaggaacgc agcagtccaa gcgcctcctc gccggtctcc gccgtctgca ccacatagcc
    52621 cttgtcccgc aaacgtaatg caaaggcctc gcgaaccgac gcctcgtctt ccaacaacaa
    52681 caaatgccac gccatgtcag gcgcctccat atggacggtg gtccgtccgc atcatcgata
    52741 ggccatcacc gaaaaacgct acgttcagca tgctgctacg cctcgtgttt gagaggaagc
    52801 cacacatcga cgactgctcc cctccccgga gggctctgga tcaccagctg gccgtggtgc
    52861 cgctcaacca tatcgcgggt gatggtcagc ccgagcccga cgcccttggc ttttcccttg
    52921 gtgaagaatg gctcatagat ccgctccaga tcgtccgccg gtatgccctt cccggtatcc
    52981 gcaaatcgca tcaacacccc gggaacccgt cgggcttccg gcacaagtgt gacatgcaag
    53041 tggccgccgc ccggcatcgc atcgatggcg ttgagcacga tgttcaagca gacctgcaga
    53101 atgtgggctt tggaagcatc gacagaggga aggtccggcg cgacggaggt cttgatcagg
    53161 atctgattct tcacgatgtt cggccgcagc agagtcaaca tatcgtccaa taccgaagcg
    53221 aggtcgcagg acgtgatatc aaacgggcgg gggcgcaatt ggcccaagtg ggactccagg
    53281 agttcatcga tccgtgcggc ctcccgcgca atcagccggc accggtcgcg cgcttcctga
    53341 ggaagcgagg cctggtccgc gagatgggtg gccagactgc ccaacccgat gaggggattc
    53401 ctcacctcgt gcagaatgcc tcccgcgagc tggccgacca gctttacttg ttccgtgtgg
    53461 cgcagggctt ccaacatttg ttcgcgttcg ctgagatccg tgagaatcgc catgaaatac
    53521 tgggtcgtcc cgtcggaggc cttgacgggg ttgacactct cccagacgag gaattccgat
    53581 ccgtctttcc gccgattcac gaaccgttcg acaaagggct gcccagcgcg aatggaagcc
    53641 cacaaacgat catagaattc cggcggatgc tttccggatt tcagaatcgc cgggcgctgc
    53701 cccagagcct cttctctcgt ccatccggtc atccgctcca gagccggatt ccattccaga
    53761 atccggccgg tcgtatcggt catcatgacg ccgtcctgtg cggacaggaa caggcgatga
    53821 tagaagtcca tctgcgtttc ctcatgccac cggcgctcga ggaccgtccc gatggtgttg
    53881 gccatggtgc agagaaactc ctgctcctgc tggctgaact tgcgcacccg cttggagtgg
    53941 gccgtcatga ccccgtagac cctgtcctcg actagcatgg gcacacacat gcccgacacg
    54001 gctccatgtt cggtcaacaa ccgcgaaggc gagaagcgcg tctcgcggcg taggtcgtcc
    54061 acgacaaccg gaagacgctt ggcaatcgca tacccggctt gagaatgcgt gccgccctcg
    54121 atggtcagtt ttccgatcag ttcgctttgc aagcctgatc cggccatgat gtacagctga
    54181 ccatcgtggg cgcgcgggac caggatttta cacagctcca cttccagcgc ggcggcggtc
    54241 tctttcaccg cctcattcat gagctcttga gccgacacac cgctgacggc gagcgtgccg
    54301 atgcgagcca acaccgattg aaaccgggct acgtgggacg catcctgtgc gagcataacg
    54361 cgttcggtca catcctccag caccataagt acgccccgct caccacgata gggcacgaca
    54421 ttgaatcggc agacgtagta gagacgcccg ccgtcccggc cttcgagctg gtattccacg
    54481 ctcttgcgtt cgccaagcgg ccgattgttc cacgcaatgg catcgtcgag gagccgtttg
    54541 cgcagggcat gctcatggcc ctcgaccctc ggaagcccgc tctcgaccaa ttccgcaaat
    54601 tgagaatttt cgaagatgag aatgcctttg cagacaaacg atagtcctcc ctggatgcaa
    54661 tcgctcagcc aggccagtac ttcggccgcg gcgatttcag actggactgg ccgggaacgc
    54721 gtggtggggc tgcggcggga tgacgccatc aggtctcgtc tctatcgggt ttggcggatg
    54781 atttcattca gcgctcacag tcgcgcacgc ggaaacgggg cgcctgcgac atcgaaaagc
    54841 cgctgtgcac cccctagcaa tctagggggt gacgaaatgg tctcgacgcg tcacggctac
    54901 gtcgctgacg aacatcgcac cggaatgttt gtgaaacaac gggccaagcc ccgccaggat
    54961 gggctcgatc cgttcctcag gcacaacggt gaagacgatc acctggctgc tcgtatcatt
    55021 gaacagcaga tgcccctcat ggaatccatg atggcctttg cccgacacat tgttgatgat
    55081 ggtatacccg gtcgcgccca cccggtcgag caggctggtg acgattttca aatgttcccc
    55141 ctccacgaca atgcgaatct ctttcatcgg atgcagcgtc agcatactcc gtctcccttc
    55201 ctctatggat tcatcctctt ttgctcgaat gccgttgctt cctgaaccga ccccaccgtt
    55261 tcaccatcct tctggtgcaa aaacggcgct gattcgccaa cgaagcgggg agcagcagcg
    55321 gacgactgct ccggcaagcc ggacaccggc tgccagcccc gcatgatgtc ataatgataa
    55381 aaggcgcctt cgctcgcctc gcacacgacc agcaacaccc attcgttgtg gaacaaccgc
    55441 tccagtcccg gatgggtggc aatgacggat tcgacacggg ttctcggcgc ttcaatgatg
    55501 gtcaacagcc gcatgggctc gtggtagggc attgagccgt cgagcacggt ctgcgcggga
    55561 agtcccaggc gaagatcact attggctcca ctcatgacgc ccacccgacc gacgatattg
    55621 tgatagacct tgctgccgct gccatagacc tcgttgtcga cggtggagaa ataatgctcc
    55681 atgttgatcc actgcgcgac gatgagaggc gcggtcatga tggcttccag cagcttcccg
    55741 gaactgtcct gccggtgatc gtaggaatgg agaaaggcgc gcccctgcaa attcagcggc
    55801 cgggtgagtt cacgccgccc gacgatgagc aggttgttcc tcgacaagcc ccattccggg
    55861 cgcacctgag cccagtccac gcttcgtctg gccgctttgg tatggggatc ctttcgcgac
    55921 cacgtggcga cgggtccccc taacgccagg ccccgttcct gtgccgcctg ggcgcccgct
    55981 tcgcggagat cctccaggag ccgtaccagg tctttccgat gagtcgcagg aatatcttcc
    56041 atgtccacga tccggacatc gttccgcgtc gtatcatgaa gcccagccag gaaatgcgta
    56101 tcgccgggga ttctgatgcc ccgcgtttcc aataccttcc ggacggccgc attatttgcc
    56161 atcgacgcaa aggcacgcgc attgggcagg ccgctgttgc ctccgcacgc gccgcaatcc
    56221 aaggcggatt cgtaggggtt attgtccgag gtgctcccat gggaacagat caccacgaga
    56281 cgcgaaaatc ccgacgtgaa tcccatgaga cgaagggccg cctcgacgcc gtacgcctgt
    56341 tcggagacgg aaaagccatg ctgggtgatc cgctggagtc gcgacgacat accacgcggc
    56401 gtgaggttga aatcgcgccg gaatcgctcg tacagcgcag cgacttccac aggggagatg
    56461 ccgaggatgt gcgccacgtc cccgcccctt tgtccttccg catcttccat cacgccgaca
    56521 cggatcgcat cgatcagcgc cggggtgatg gcggaaccga gggcaggaaa ttcctgccgc
    56581 aacgccgcac gaatgaccgc gcgctgctcc accgcaacca tctcctccgc ctcttctctc
    56641 gtgagcttat cgatcgtgag cgtggtggcc aggggcggaa cccacaggct ctttaacgaa
    56701 gacgtgagac gttggtaggc aagggggcac agggttttgc cgaaaaacgg cagactgaaa
    56761 aaccatccca gtgcttccac catgacatag ggggtaatga cgttttcttt cagatcgtgc
    56821 aggagcgtat gcgccacgtg gctgagctgg gcaaacaacc gatgtcgact tgccgccgcg
    56881 ccatggtaac tgcgtggaat ctcgcggatg tggttcttgg gttttaacaa caccggcgca
    56941 tgggtgaccg gatgctcttc tccgaacgcc tgatacttca ccggtatgcc gaagaacccc
    57001 gccaccccaa aagtctcata acctcccaac tgctccagat gccttcggaa cacctcggaa
    57061 cggacgtcga tacaaaacac catttgcgcg agcgggcgcg ccgatccggc tggtgcagct
    57121 cgatcatcgg tggcgcgaag ttgtttcgtc acggcccgca attcgtcaac tacgtcgcgg
    57181 cgatgcttgg cttcgagggc ttccagccaa cgctggcctt gttggccggc gggaaatcca
    57241 tccagccaat tcaacacggt cgccgcatcc gccactgagg tctgactgat cagcgccgga
    57301 tcagtgccga gcgccgcggc cagagcaagc aaccggcggg cgtcccgctt catcgcttca
    57361 gtgccacgcc actcggacgt ttgctcatag agacggctgc cgacctggtt ccattcatcg
    57421 agcgtggcag cctggtggga ccgacgccac tcttcggctt cccggatcgc ccatggtggg
    57481 atggttctgg ccgtcagctg gcgccgtaac caatgggcat agggggcctg atcaaggatg
    57541 gcgcgaatgg catcgtactg tcccggccga tttaacgcgc gcctgcagtg atactcgaca
    57601 aattcgcgct cgtaaaagag ccggaccgcc aagtacttca cgaggtcgat cgggtaccgc
    57661 acctgccacg gatggtgctg ctcctccgct ctccatttga tatatcctgc ccagccaggc
    57721 aaggcggaga ggtgcaaggc gaagtaggct tcccaggcag acttcggtat tcccagatcc
    57781 accaggcaag agaggaccgc ttcttccgga cgctcgccta aagcatcaat ctttgtagcc
    57841 gcatcctgaa tccccagcag ccccaagccg gcatcgtaac gggccaagcg tttccaggcg
    57901 cgataaaacg tctgctcccg gttgggcatg acccaggacg cttcgccttc gtccagaaag
    57961 acgctgcacc acttgatcat ctgctcattg atgtccgaca cgatgcaggt tcccaaggtc
    58021 tggtcgcacc aggccgagag cgtctcatgg gacaagaggt caagcgactc ccagggcacc
    58081 aatggctccg gctgacgaat ccgcgtcccg gccacggcgg taccgagcca ggccagtaga
    58141 tcatcgagag cggtatcccc ggccgcccca gagtcctgca gtttcgccgg taaatcgacc
    58201 aggccatgca ccatagaaac gcgcatcagc tccagatgtg tcaattcgcg ccccgcaaag
    58261 gtcacgcacc ggtcgctggc cagaggcgcc agcacgcagg acacgtcatt caggccgatg
    58321 cggccttgct cgaagaaacg gcggtagatg gcattcgaca gatagccctt tccacccaac
    58381 aaatgctctc cgcgtttgat ggcctggggg aaaggcatat cctccattcc atgcaacggg
    58441 ttatggtgaa tgaaggtccg catgggccag tactgggcaa tcgcttctcc cgccagctcg
    58501 acatgcgagc gcaattccat gcgctgggct tctgtgaagg cacgtgtttc aatggccatt
    58561 cagtgactcc gacttcgatt ccattgcggg gcatctcacc ggcgcgatcc cgcgcggccg
    58621 atcatgcgca ccatgtcctc gatatagagg ccgttcagga aggcgacgta ggcacgagtg
    58681 cgcaaggact ccatccaccc cggcatgagt actttgatgc ccttcgcttt tccgtagatc
    58741 agcccccagg ctccgacgat catgatagcg gagactccca ccaccgcgtc gaacagggca
    58801 cgattccatt ccgcagcctg cacgtacgac gcggcaatgc cgtgagcggg atagaggaaa
    58861 tgcgtgaagg cttcaccggc ccagagatac gtcaaaccga tgagagccag cgcgccgagc
    58921 atggtcaggg agacattcca agacgctccg gcgtgcaggc gatacaggct gaacatggcc
    58981 tgcgaggtcg tcacccaacc gaagaacagg aaaatgaccg taccttgcga ctcctgcaaa
    59041 ggaatactca cgacgccatg cgccaccagc agaataatca acggcaacac cagcgtcacg
    59101 gcgaggcctg tggtccaggt catcggtgaa aaggcgcggc tctcctccac ccggttgcca
    59161 ggaggcagtt tgaattccgt cctggcctta tgaatctgcg cgccggagtt gagaaacagc
    59221 gtcgctttga acaggccatg ggcgcagaga tggaacactg ccagcgcgaa ggcccctagt
    59281 ccacattcca tgaccatata gcccatctgc cccatcgtgg aatagaccag tgtgcgcttg
    59341 acgctggact gggtcagcat ggcgctcgcg ccgattaacg ccgtcaggcc gccgatgacg
    59401 aacaacaggt gaagcgtggt gggagcgaga ccgaacagcg gcgccattcg gttgaccaga
    59461 aatccccccg cgtttacgat tccggcatgc atcagtgctg atacgggcgt gggcgcttcg
    59521 atggtgccga tcaaccagaa gtggaacgga aattgtgccg acttggtcat gaccgacaga
    59581 accaggagga gcgtcgtgac aagagggacg ttcagttcga gcgcgagacc ggaccagctc
    59641 gtcaccggtg ccgcctgcac tcgcggaaac agctcggtca cgtccaacgt gccatacgaa
    59701 ccatagagga tgagcaagac cagggcaaag gcggcgtcac ccagactttg cacccagaac
    59761 gtcttctgcc cggcgtccct cgccgcgagg ctcgccgggt tgcacaccaa caaggtcgcg
    59821 agcaaccacg tcaccagatg ccagcaggca aacatccata acagattgcc gctgctgacg
    59881 aggcttaata acacaaacgt gaggctgccg agcagagcga agaagcgggt ataccccgca
    59941 tcgccgacca tgtaccgctc agagtacaca tgaatgacca agctcacgga actgatgagc
    60001 accatcatga ctgcagccaa ccgatcgacg agaatggtat acgaccacgc tcctgaggga
    60061 gcgccggcga gcgtgaggcg gatgggatcc ccataggaga ccagcgccag cgtgaccagc
    60121 gatgtcagcg cggaggccca taacgctcct atccccagcc gtgacactcg ttcgcccatc
    60181 tctcgccaga agagggcgat tgatagcgtg ccgaagagtg gcgccagaat cgttaccaat
    60241 ggaaagattg cttccatcat aggtggctct ccaaatgaat catcatgctt cctctacagc
    60301 aagaacaggg cctgattcag acgtgcatca gactccgaat gaatccggaa aatgcgtgcc
    60361 tgtagaggat gtccgctgca gccacgggtg cctcaacgct taagtattgt gcgcggacgc
    60421 acagctgtgc atatgcgcac aatcaggaag ttaagtgcaa gtcaggctta cactgatgtg
    60481 ggactcggca gcggggcatg ctgcgtaccg acgcgccttt ccccctgcca ttggttcccc
    60541 agctgcggcg aggctttaca ccccgtcaca cgtccttcga ttcggaccgt ggcttgatgt
    60601 gataaccggc ctatgatagc gtttgcaatg catgctgtct gcaggacaaa ttcgcaccag
    60661 tgaacgccgt cggattgcac acgtcttgcg cggctggatg atcctgtggc tgctcgcctt
    60721 gccgttgttc cacatccatc ccgaaacaga cccacatcat ggggaggccg gtcatgtcca
    60781 tgccgccgca gtgcataccg tcttctccgg tgacctcgaa ggcgaattcg gcgaacatca
    60841 agagacggcc tctgtaagac ctcatccagg tgaagggccg gcggtttcag ccgaaggtgc
    60901 gcatgcctgg agttccgatc cggaactcaa cctgtctcta ctcaatgacg ccaccgaccg
    60961 taagctggcc aaaccgctac tgacgcatct cctgtttgtc gcccatcatc tggtacccct
    61021 ctttgaccag caggacggtc cgcacggtga gccattgtcc tccaccgtca ccatgctctt
    61081 caccagggat gtgccggccc gtgctccgcc ctccccgttc ttcagctaag tatcacccgt
    61141 tttcaccgca gacattctga tcgcacgcac gtctccgcga ggcctgttcc ggctcccgcc
    61201 cgacgatccg tgcggaatgg aggaggtttc cgatgatgcc tcgtatggtc ctttccctgc
    61261 tgatggcctg cgtcttcagc caatcgggcg ccttgtcctg ggctggagaa gaacggcctc
    61321 gcgcctattc gctgtcggac gttctgacgt tcgcggcgca acacaatccc gtgctcgcgg
    61381 gagctgaggg actggtgaaa caaagccagg ggcaacagat tgctgccggc gcctatccga
    61441 atccttccat caccgggatt gccgggcgcg gcgcgatccg tgaccccagt accggaaccc
    61501 acgtcaccga gcggacgttg accgtggaac aaccccttga atggaccggc aaacggcagg
    61561 cccgccagga agcggcggat gccggcctcg ccggcgcgaa cgccgctctg gaggacgccc
    61621 ggctttcatt gctcgctgat gtcaagattg cgttctatca cctcctgttc ggccaacgcg
    61681 atgtcgaact gtccgctcaa aatgtcacga atgtggaaga ggtcttgcga acggtgcagg
    61741 cacgcgtgac ggccggtgaa gcgacatctt tcgacagtct gaaagccgga gtcgaggtgc
    61801 aaaaggcgaa gaaggaagtg gctcgcgcca acaacgcgct gctggtggcc agggcacggc
    61861 tgaataccct cactgccggg acactcggca aggagttttc cgtgcaaggc gatttcgacc
    61921 ggccgaagca gagcctcaat gcagatggtc tgaccgcgca ggccttggag caacaccccg
    61981 ccgttcgacg cttgagcaaa ctggcggagc aggccgacca tacgctgcgt tttgagcgag
    62041 aggcccgcgt tcccaacgtc agtgtgatcg gaagttacca tcgcgaggcg ggcgacgaat
    62101 ctctcacggc cggcctggcc gtgccgctcc cgctgtggta ccggcggcaa ggcgagattc
    62161 agtccgcatt gggcgccaaa caccgtgccg atgcggagcg cctgcgggct cagcaggaat
    62221 tggagcaggc catcacccaa catgcccagg aagtgcggac ggcccaggag caactgcagg
    62281 tcttcgaaac ggggttgctg aaacaggcgg agcagaccct caccgtggca cgaacgagtt
    62341 tccggcacgg cgcagccagt cttctggatg tcctcgatgc gcaacgggtg taccgccaga
    62401 ctcaactcga atatgcccag gtgcgagcgg atctctccat cgcactggcc cggctcgaac
    62461 gcgcgctggg aacatcatta tgaacgccga ccgcagtgga tggactattg gagtgccgcg
    62521 atgacgagac gactgatgac gatcggcatg gtgcccgcgc tgctctggct tctgtctggt
    62581 tgcagcgacc agggaacgcc gccaccaaaa cctgccacga ccggcgaagc ccagacaatt
    62641 accggtagca gtaaagccat ccacccgccg ccggcagtcc aggctcgcct tcgaagcgaa
    62701 cctgccgccc tgcgtgcggt acccacactg atcacagctc caggtgaggt ggccctcgac
    62761 ctgaagcagg tcgccaaaat cacgtcccgc atcggcggac aggttgagcg tatccatgtg
    62821 caattgggag atcgagtcaa gaaaggccag gcgctggtcg ccatcggcag cctgcaactc
    62881 gatcagttga tcgaggaata tctcgtcgga aaggcccaag ccgacgtggc ggaaaacagt
    62941 tttcgccgga ccgagaaact gcgcgccgac gacatcgtca cggagcgcaa gctgatcgaa
    63001 gacaaggggc tctatctcga gacgcaggcg cgttatcaac acatccgcga acgactgtcg
    63061 aacatgggca tctcgacgga tgacttgaaa caaggccccc atgagaagag ccatctttac
    63121 accctcaccg ctccgatcgc agggaccgtc gtcatccaga acgcggtgcg cggacaggga
    63181 gtcggccccg gtgatgaatt gtttgagatc gtcgatacca gccgtgtctg ggtctttgcc
    63241 aatcttccca tcgagcaggc caggcaattc aaggagggcg actccggtac catcacgccg
    63301 aaaggcggcg aagccatcgt cgcgccgctc acctacctgt cgccggtggc ggacgagacc
    63361 acccgcacca ttcgcgtgcg gttcgaggta gccaatctcc aaggacagtt gaaaccacgc
    63421 gagtatgtcg aagtggcact cggctggtcc ggggccccag tgatcaccgt tcccatgacc
    63481 gccatcacca cgatcgacaa aacacgcggc ctgtttctcg acaccgcgga tggctacacg
    63541 ttcgtgccgg tcgacgcagg ccgcgaaggc ggcgggtggg tggaaatccg tcggggtgtg
    63601 aaggaaggcg accggatcgt gaccgacggc gtgttcgatc tgaaaaatgt gctgctgaag
    63661 gaacacatcg gctcgggcga atgaggcgcg catgcaacag ctactgacgt tttcactccg
    63721 ctatcgattc tttacgctcg tggccatcgt cgtcgtgatt gcctccggcc tgtggtcgtt
    63781 cgcacacctg acgatcgatg ccgtccccga cctgacccca gtgcaggtgc aaattctcac
    63841 acgcgccccg gcgctggggc cggtcgaggt ggaacaattc gtgacgtttc ccatcgaagc
    63901 ctccttgaac gggttgccgg ctctgcgcga gctgcgatcc atttcgcggt acggcctttc
    63961 cgcagttacc gcgatcttcg atgatcgcac cgacatctat cgagccaggc agttcgttgc
    64021 cgaacgattg gctcaggcca tggaacgcat cccggcggaa tacggccgcc cggtgatggg
    64081 ccccctcacg accggactgg gcgaggtcta tcaattcacg gtgaaaggcg aaggctttag
    64141 tccgatggcc ctgagaacgc tgcttcagtg ggacatcggc atgaagttgc gcgccgtgcc
    64201 cggcgtagtc gaggtgaata tatggggagg cgatccgcag cagttccacg tcatcgtcga
    64261 tccctcgaaa ttgttgtcat tcaaattgtc catgaagcag gtgttcgatg cgttgcagcg
    64321 caacaacgcc atcgccggcg gcgggtatat cgaacaccag cgcgaacaac tgtcgatccg
    64381 cggcgaagca ctggcaacac agctcagcga tctggcccag atcgtggtag cccacgggcc
    64441 tggcggcgtc cccatttata ttgctgacct ggccgaagtg aaagaaggat cagcgctcag
    64501 gatcggagcc gccacggcca tgggtgaggg cgagaccgtc atcggcatgg tgcagatgct
    64561 ggccggagaa aatgcgcagg aggtggtcac tcgcgtgaag gcgcgggtgc aagacattca
    64621 ggcgaccctc cccgccggcg tcaccatcga accctattat gatcgcacga tcttcgtctc
    64681 gaaggtcatg cagaccgtcc gcaacaacct cctggagggt ggtctgctcg tcatcgctgt
    64741 cttgtttctc tttctcggcg acctccgcgc agggctcatc gtcgcctccg cgattcccct
    64801 cgccatgctg atcgccttca tcggcatgat gcaggccggg ctctccggca atctcatgag
    64861 cctgggtgcg atcgacttcg gccttctagt cgatggctcc gtcgtgatga tcgacaacat
    64921 cctgcgacga ctgggcgaga agccgccacc aaccagggaa gcccgcttga aggaagtgct
    64981 ggccgccggg caggaggtgt tgcgccccat gacgctggcc gtgacgatca tcattctggt
    65041 gtacgtcccg atcctcgcgc tgaccggcat cgaaggcaag atgttccgcc cgatggcggt
    65101 gaccgtcatc atggccttga ccggttccct cttcctcgcc ctgacggtca ccccgttgct
    65161 gtccttttgg tttttacgcg ccactcccgg cagcgaaacg acggcggtca tacggggcgt
    65221 gagaagagtc tatgccccat ggctgcagcg ggccgtggcc cgcccaatct ggcccgtgtc
    65281 gattgcggtg gggctttttg ccgtcagcct cgtggccgga agcaggttag gaatcgaatt
    65341 tgtcccgcgt ttggaagagg gggacctcgc ggtgcaggta tggcggctcc cgagcatctc
    65401 gttgagcgaa tcggtcacca ccgcgttgga tatcgaacgg gtcctgaggc gttttcctga
    65461 agtcacgcgg gtcgtcacgc gcaccggcag ccctgaagtg gccacggacg tcatgggcgt
    65521 tgagatgtcc gatgtcttcg tcatcctgaa accccagcgc gattgggtca cggcacacag
    65581 ccgcaacgaa ttgatcgagg cgatgaaacg acagatcacg gaccaggtac cgggggtcgg
    65641 acttggattc acccaaccga ttgaaatgcg cttcaatgaa ttgattgccg gcgtgcgttc
    65701 ggacctggcg gtcaaaatct tcggtcccga tctcgacatc ttgaagcagc aggctgaacg
    65761 ggcggcgcgg gcgcttcaac atgtaccggg agcggctgat gtcaaagtgg agcaggtcgc
    65821 cggcttaccc ttactgcgcg tcgttgtcga tcgcgctgag atcgcccgtt acgggctcac
    65881 ggctgaagag gtgctcacgc ttgttcaatc ggcccgggtc ggaacgattg tcgggacggt
    65941 cgtgcagggt tcccggaggt tcgaattagt ggtgcgattg gcggaccggg tctcgcgtga
    66001 tcctggttca ttgggcagtc tgctcatacc gaccatgcat ggagaactgg ttccgctctc
    66061 acgcgtgacc agcatcctcg tcgattcagg tccggcacag gtgagccggg agcatgtaca
    66121 gcggcggatt gtggtcgaga ccaacattcg cggacgcgat ctcggcggat ttgtatcgga
    66181 ggctcaacag gccgtggccc aggcagtcac gctgccacca gggtatgagt tgatctgggg
    66241 aggacaatac gaacacttgc aggaggcggg caagcgtctg gccatggtcg tgccggtcac
    66301 actgatgctg attctcggca tgctatctgt catcttcggc acgctacgcc cagccctcct
    66361 cattttcctg aatgtcccac tcgccttgtc aggcggcatc ctggcgctgt ggctgcgggg
    66421 attgccgctc agcatctcag ccattatcgg gttcatcgct ctgttcggca ttgccgtcct
    66481 caatgcggtc gtcctggtca gccacatccg ccagctggag gcggaagggg tttccactcg
    66541 ggaagcggtc ctgcacggag cgatggatcg tttgcggccc gtgttgatga cggccctggt
    66601 ggcaagcctg ggctttctgc ccatggcctt ggccaccagt atgggagccg aagtccaacg
    66661 gccgctagcc accgtggtga tcgggggatt gctgacatcg acagccctga ctctgctggt
    66721 gatcccggcg ctgtactcgc gcctggtccc gcaggctgcg tcccggcccc atccggcagc
    66781 tgtacaggcg gacgatagcg ccattcaggt gtaacccccc gtggcatcac gagaaatcga
    66841 ccaccagccg cacgcgatac ggcgccgctt ccgactcttt tctggacaga ctttcatccc
    66901 ggcggtaagc tttcctctac gaggcgaagt gtctgcacgc aagccagggg cctgcgcagc
    66961 cgctcaagcg aattcataac cggcccattt tcccgaggag tagtttatgg cgaccatcat
    67021 ggtgatcgac gacgaacctt ccatccgcgg cctcctgcgc gaggtcctgg aacgatcagg
    67081 acacaaggtt gtcgaagcca aggatgggcg tgaagcgttg gatctctacc agaagcataa
    67141 ggccgatctg ctcatcatgg acttgttgat gccggaagtg gatggattgg aagccacgtt
    67201 gcagctcacc cgcgaatata tggacacgaa aatcattgcc atgaccggcg ctcaggggga
    67261 tcggaatttt ctcgatatcg cccggctctt cggcgcgcac cggactttcg aaaagccctt
    67321 cgacttgaag gaaatgctga acgccgtgga agcggagctg gcgcagaaat aacctcgcac
    67381 cggccaatcc gccggctcac ctgacgcact cgtcactccc gggaaaaact actggaatca
    67441 ccgccgctgc ttctgcaaga attcaccgtg cagcagaccc ccacagccag gttctcgtgt
    67501 ttctaacccc cactcgcgca tggcgtctct ggtgtgcctg gagcatgctg attgtgctcg
    67561 tgggcatcct gcctctccat aacttcgtcg gccatgcgca ttggcagtat attcgttggg
    67621 tgccgacgcc ggatcaattg agttccccgg cgatcctgct ggatcttgcc ttcgatgcgg
    67681 tcgccaatat cctgctcttc gttcccttcg gcctgctcta cgcgcttcgc ggcctggaag
    67741 gcaaacctct ttctccgcgc catctgttgt tgatggcctt tgcgctgtcc ttcagcatcg
    67801 agtactacca ggtctattgt cataaccgca gcacgtcgct gctcgacctg ctggacaatg
    67861 tcttgggcgc atacctgggg atgaaagcgg gggaagtgta tatcagaaag aatgcgtcag
    67921 cagtggtcga accggccgca taagattatt gccagcctgt cgggtcatag ccccggtctc
    67981 gtaagcaccg atcgacataa ttcgtaaacg ccgcgccggg cggagaccgt ttgaacagtc
    68041 ctcgcaaaaa ccctgcggta gcgccgctgg ctgcgccgac cattgcaccc cgtcccgggt
    68101 gtccgagaat ggctccgccc accgctccgg ccgcagaacc gacagcgccg cctcccactg
    68161 tccccgtcac gacctgggcc gtcttgcctt cactggaaga cgccccggcc tccttcgcca
    68221 gcgccatgca atcctggata tcagcctgcg cggcatcggc cccgacttgc tggtagtgag
    68281 cattgggata cagaatgggt ttgggggcag cgcagccgga aagcacgaga cccagaacac
    68341 agagacaagc catttgcctc atgacacaga cctccggtgc gctgaatgtt tgaagggaac
    68401 gtcaggccgg cccggtgatg gtacggtata aattagatcc ggccacccaa gtccttgcgc
    68461 tgcagcgtac gcatcatgac ggaatcgttg tacgccacct cgctgagcgc atccatgatg
    68521 tgcagccctt tggcagtgac caactctttg gccttggcat aattcttcgc actgaaccgc
    68581 gcgaccgcag gctgaccatt cacaatctgg caggccagga cttcaccgcc ggcgacatcc
    68641 atcgttcccc cttcttcaac cagctttttc gcctgcggca tcgtaatctg atgcaattgg
    68701 gcaaattcct ccagcccgcc ggtttccacg agacgtccat cgggcatgat gcacaaccgt
    68761 ttgaaatggg gagaccagtc tttcctgaaa tcgatgaact tgatgtcgcc gcctttggcc
    68821 acggcacgat ccagcgtgcg gtaatcgagg ccaagatttt tctgggcgag tttggttttt
    68881 aattcctccg gcagcgtccg gtaaaagatc tctccggcgg catcgatcaa tgagacggcg
    68941 cgcgccctgg cccattgctc cgcttcggcg aactgcatga gatccagcac ccaggtctgc
    69001 ttgggagaag cccccgtcgg atctacccgg caggcaaaga gcccccgcgt gagcagcccc
    69061 cccacttggg gatacacgta cacgcccccg tctgtcagga gctgcagcat ctgctccaac
    69121 ggcacatcgt aactttccgc caggatcttc gcatgcgccg ccagatcttc cggcaaggtc
    69181 atggcgcaca ccgtgacgtt tcccggcgca tcgaattccg agtaatcctc gatcacgttg
    69241 tacagaatcg gcggctttgg cttctcgatc ttcttcttga tcttgaacat aatgaattcg
    69301 ttcctcgtcg ttcgtctttc gttcacacta cgtgcggtaa attactgcgg tgcaatcacc
    69361 cgcgctggag gtggtagtaa gccggcaagg tcagcagccg cccgtcgtcc accggtccac
    69421 caatggtaaa gtgatagagt gattgcagcg tcaggtcctt gatgccgacg atctcgtgca
    69481 cgggatcatc aaaataacag ccgatgcccg tccctcgcac gccggctgct tctgcctcga
    69541 gatacaatac ctgcccgacc agccccgctt cccagaaaag gcgtggatac caccaagccc
    69601 cgccctgccg caagcgcccc tcaaattcgg ccaacatgcc gagtgaaaac gcgctgtcac
    69661 cggcgatgcc ttgatgacaa ctgacttggg cagccaggcg ttgcgcattg ccttgcagca
    69721 gccagtacag cggaagatct gtcggacacc caggcaccgg ggtccactcc aattcaggat
    69781 tcatcgcctg ctgcagaagc ggcagcttct tcgcatcccg cgccatcaca tagaggcccg
    69841 gcgtcaggcc ctccacccgg tgcacgaaca acagcagatg aatcgccggg tcccagggca
    69901 agagatccca cggcatgggc cgggccagtt gcggttggct ggcgtgaggt atcagccgaa
    69961 ggagaatctg aaaaaacgta gcgctggaaa ttgccgtgcg gccatcgaac gacacggcgc
    70021 tgcgacgctg tctgatgatg gtcgcggcgc cgcgcgtggc ttgtgggtcg tgacgtggtt
    70081 ccgaatgcac gggcggctca acggggagag acaccaccag gcgctcttca cctgttttcc
    70141 atgaggcctc cgctgcttcg tcgatcgctt cccaatgcac gccatgctcg cgactcaata
    70201 ggttcgcatg gccatgccat ggtcccggcg ccagatcctt caccaccgtt tgatccacac
    70261 ccaacggcag gttttgaaca tcgtgcttca tgttgggtga aggccagatc acggcaagac
    70321 agtcgggatg ctccggttcg gcctcaccaa aatcctcctg gcgatgggtg cccaacagcc
    70381 gcgcgacctg attttgttcc accccgtcca gcagcaccat cctccaacca agcgtcgccg
    70441 cggcgatacg cgcggaacca agggcgtgcc ccacgtcgtg attgcaatag cgaaaggccc
    70501 gctctccata cttccaggct tcgcgccaat ggacggaact gaggccgaat agaaatgcgc
    70561 catccggaaa cggcgcgagc agctgtgtga tcttcccgga cggaaggccc gcgcggaact
    70621 ccaagccatg ttccttggct gcatagtgat agaggccggg cggcagatcc aagccatcga
    70681 ccggcggaac gatcacatag ccttcggtcg gatgcagatt gccggacgaa ggattgttgc
    70741 gaagagccca ctccgactcc cctgccctct tccaggccga cagcccgagc gccagttcaa
    70801 aaaaccgtga gatcgtcttg atggtcaatg gccgggaggc cactgcgcct ggttgataga
    70861 gagcatcgta agagggcgac tgcggatcgt catccgggct cagcaacggt aaacggatca
    70921 gttgcgcacc ctcaaaccgc cgaaacggat tgggttgatt tgcccaatcc agaaatccca
    70981 acgatcgcgc gtatcgattg aaatgatgct tggttcgaat gtgataggcg atcacacggt
    71041 cgattggatc tgtcgaagta gtcccggcag acgcgccgga agacggttgg tcgagagtca
    71101 tgcgcgcagt ctaacgaggc ggaggaagga aagtaaacgc acagtccgtt cagcccagga
    71161 gtcttgcgat cccgaaggcc gccgctgccg cgagtcctcc gacgaacacc gtttggactc
    71221 cgccacgcca gggcggcaca ccggtcaatc cacttttcac gtatccgaaa acaaacaagg
    71281 ccacgggcgt cacggcaatc gaccaccaca gcgccgtcgc aatctcgtgg aacaccatgt
    71341 agggcaacaa cggaatcaat cccccgatca catacgagag cgcaatcgtc caggcgctcg
    71401 acctggcacg cgccggatcc ggttcttcca atcccaactc aaaccgcatc atgaagtcga
    71461 cccaccggcg ggggttggct cgaagggagg cgatcagcgg agcaatgtgt tcatcgcgca
    71521 acccgtagcc acgaaaaatc tcggccacct cctcggcctc cgtttcggga atatgctcag
    71581 tctctcgcac ttctcgcttg cgttcagcag cgtaatgctc aagatcggtc ttggccgcga
    71641 gatacccgcc cagccccatc gcgatcgagc cggcggcgat ttccgccaat cccgccgtaa
    71701 ctactaaagc ggacgaagcc accgcacccg acaatccggc ggccagggca aaggggaccg
    71761 tcaacccgtc ggccatgccg atgacgatat cgcgcaccgt cgccgtggcc gtgaaatgtt
    71821 tttcactatg tggagtcgcc ggcataggga tactccttcc
//
LOCUS       DAOQEW010000002        69271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_63_length_69271_cov_8.953320, whole genome shotgun sequence.
ACCESSION   DAOQEW010000002 DAOQEW010000000
VERSION     DAOQEW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 69271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_69271_cov_8.953320"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLS35_00320"
     CDS             complement(<1..1531)
                     /locus_tag="PLS35_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17115.1"
                     /translation="MTIRRMLQRLLHSPQPGEAPDAPLESEQVIARQQYESSLRESEE
                     KFRRLFESSRDAIMILYPPNWNFIACNPATVQLFGARDEAHFTSLGPGDVSPEYQPDG
                     ELSMVKARKAIEAAMREGFHLFEWMHKRVGGPTFLATVQLTRITLQGVEGLQATVRDI
                     TEQRRAETELRAYATFQRAILDHAGSAIISCQPDGIIQLFNPAAEALLGYTADELIGR
                     QHPGIFHVREEIVERARQLSEEFGCTIAPGFDVFIEKSRRNLPNEHEWTYIRKDGTRR
                     TVLLNITALRDADSKITGYLAVASDITPLKRTAQELLLAKEAAEAASRTKSQFLANMS
                     HEIRTPMNGVLGMTELLLTTALDTRQRHLTRTIQQSGEALLAIINDILDFSKIEAGKL
                     QLERLDFDLQDTVDNAVELFATPAQRKGLELTCHMPSSIPRTLRGDPIRLRQALLNLI
                     SNALKFTSTGEINVRIDAVVETDTSVTLRFAVKDSGVGIPAEAHQRIFEAFSQADGTT
                     TR"
     gene            complement(1545..4454)
                     /locus_tag="PLS35_00325"
     CDS             complement(1545..4454)
                     /locus_tag="PLS35_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17116.1"
                     /translation="MPWFHHLWMMWQGSLRLRLSVGMAVLITLGMTVFATIRLVEIRH
                     SLVDAAQARALAVSRTFAMMGAAAVLDNLFRIQEALTRYANEADIVGIVIIDPDNMVV
                     AATKPHDIGRELTDHTLSLAQTSGSETMAHRLSEDGTPTLIVVTPLKNEDDIAAWVRI
                     EFSLAAMQGELVREVRRLLVLSVLLMSVTIVVGQIGIRRMSALFRDTAAKLQDTLHTL
                     HRPLKEDAGNATSLNGDSPGPASYTGELEQMVDLVETTTALLRTQAQRLQSFTDSLED
                     AVRQRTTELHQAKDAAETANQAKSQFLANMSHEIRTPMNGVLGMTELLLTTALTPKQR
                     SLAKTLHQSGTGLLDIINEILDFSKIEAGKLKLECIEFGLRQTVEEAIDLVAETAHRK
                     HLELTCFIPEEIPDAVVGDPVRLRQILLNLVGNAIKFTQAGDVSVTLSLVSQEASRVN
                     LKCCIRDTGVGISDEAKARLFQAFSQADGSTTRRFGGTGLGLAIVKQLAHLMGGDVGV
                     DSIPGQGSTFWFTVSMEYASQAVSAHEGLASPLAGHSILVVDDNSTNRHILEAHLSNW
                     GATVQSAASSEDGLRLLTEAKGGNQKIQMAVLDIRLPGMNGIDLARKIREDVDLRDMA
                     ILALSSEDRETDESHHHPKLFNAWLRKPVHQSLLKDCLTRLCLAPSAEDAPATIQPPE
                     QTRTLAGHILLAEDNPVNREVACTMLEILGCTTAVAEQGKEAVETAAHGAFDLILMDC
                     HMPEMDGLTATGLIRAHEQQSMPARHTIIVALTANALEGDRERCLAAGMDDYLTKPFT
                     LDALKELLQRWLPEHVPSDSTSDSQPAEVSNGVPPQQAVSSHAQPLAAESEHVDAHAW
                     EAIRARQRPGQPDFLHKALSLYLPYADKQIARLENAIADQDARAIATIAHTMKSSSGK
                     LGAQRLALLYTELESASRAGRIGDFQELYDRLIPEHRAVSALMRDELNGEGRSAA"
     gene            complement(4456..5175)
                     /locus_tag="PLS35_00330"
     CDS             complement(4456..5175)
                     /locus_tag="PLS35_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17117.1"
                     /translation="MVMRLMLVVLGLTLGVPSLLQAESPTGKLAGRITYSGSALPDQT
                     AEITRDSAFCGTSTTIKDVVIHPKTRGLEGVVVSIESGNLPSSDAPLAPLVLTNTHCA
                     FLPRTVIGQVGQQLEIRSDDPVMHNTHISLGSRTFVNVAMIPASRPVRKPLKQPGIYT
                     VKCDAHKFMHADVIAFAHPFFSQTDESGSFQITNLPPGEHVVNIWHHTLGSYQQTVRV
                     SAEAETTIAFDFAAHAESNKK"
     gene            complement(5256..6029)
                     /locus_tag="PLS35_00335"
     CDS             complement(5256..6029)
                     /locus_tag="PLS35_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI17118.1"
                     /translation="MGISMYKYLVILAVSGLCATSAYAVRPEDNRDTLAEGRRIYERA
                     CAWCHGNGGLGDGPSGWSIGRYSAPRPRDFTKESYKLRSTPSGELPTDQDLFRTLTQG
                     IPGVMPSYRSLNESERWQVIAYLKSFNPAFEQETPVSIPLPPPPVSRPEGAVQHGRVL
                     YAKYDCRVCHGANGEGNGPESQAGRLRDTNNLPIRATNLTDPASFKNGSTAQDLYRSI
                     MTGLDGTPMPSYANEFAGQEEAVWDLVWYLQSLSGRPEK"
     gene            complement(6054..7355)
                     /locus_tag="PLS35_00340"
     CDS             complement(6054..7355)
                     /locus_tag="PLS35_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17119.1"
                     /translation="MSVPTADAIPSFARKYALDCTACHTAPPRLNTFGERFLENGYQL
                     PGTTDGGLTGKKNLGDVSLDDVANYTGVRLRGNVLRTHHFKQQNPSGAEAGTVQNKSD
                     LAFPEVFSLFTAGTLAKNIGFFAELESNLEASHTGIERAFLTFNNIGGENVANIRVGK
                     IDPSAAFSFSTLRQQLDFIAESTHPSTNAVQRAGLFPLATAAKFYGLRNRSGGTISPY
                     APSLYNAVVETGAEVRGRPFGDWFMYQLGVLNGANEGFGDSNKGKDFYGAIRFDYARS
                     ANFSASLSGFAYLGNSNATVFDGTRTTDVNWSRYGVAAHLRYKMFDLHGMYTLDRITH
                     APATALSNFDTDASGLTVALDTHVANNTLLSLRYDTMDAGGDLSQRSSQSFLGAQVKH
                     YVRSNIALYARLDHNLRHAEDGQAAARNLREAFFAGIDVAY"
     gene            complement(7662..8090)
                     /gene="queF"
                     /locus_tag="PLS35_00345"
     CDS             complement(7662..8090)
                     /gene="queF"
                     /locus_tag="PLS35_00345"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNI17120.1"
                     /translation="MREQNKKKLKTGNSTTKLGYNERHARSGISAPLPDIETFPNQYK
                     GYEITIVIPEYTAICPKTNLPDFGTITLHYKPNKQCLELKALKMYVHAYRNIGIFYEN
                     AVNRILQDVVRACRPTWAKVTGEFAARGGLRSVIEAKYPE"
     gene            8831..9730
                     /locus_tag="PLS35_00350"
     CDS             8831..9730
                     /locus_tag="PLS35_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNI17121.1"
                     /translation="MSLDYVKFSSGFEKFMPKEYRDMVEHGPFGKKVSVSQMGSFKEV
                     LEEHPMCAGCAMTLFIRLAMIAFPNPEDTITVGTAGCGRLAISQAAIPFVYGNYGDQN
                     GVASGLSRGLRLRFGDKPKDVVVMAGDGGTADIGFQQVLHSWFRKERFTTIMLDNEVY
                     GNTGGQESGMTNRGAVLKMAPLGKKFEKMDMLQMAKVAGCAYVATVVPNNPRRVESVI
                     KKAVLIAREVGSTYIQAYTSCNIEYAIPTDKVMEDAKNVENDRYQFSEYITDEAKQFL
                     TERYGYKEFLPKPAAPAPGLPKA"
     gene            9754..10467
                     /locus_tag="PLS35_00355"
     CDS             9754..10467
                     /locus_tag="PLS35_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNI17122.1"
                     /translation="MATRFNIRMAGVGGQGVVTGSHILSTAVIHAGGESTIVPFYGSE
                     KRMAPVESYVRVSDEPIYEIGEITFPHIIIIFHPQVITHGKSYTMPFYFGLKENGIAL
                     INNDGPMKLHRDQARELEERHAKLYYFPATKLSLEVAGMDLATNMALMGCIGAITGLT
                     TVEALEQSVKDRFLGKGFVVSGGTAALDSVVERKFKKKQELIEKNVAVIRAGWNYAVD
                     HGWAAPSVKRSEATESVTA"
     gene            10493..10816
                     /locus_tag="PLS35_00360"
     CDS             10493..10816
                     /locus_tag="PLS35_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17123.1"
                     /translation="MYLVADISVEICAAKSCKLCTQYCPEANTILYSDEMGKDKGFKY
                     GSAYVAVDRCKGCAQCVWVCDNMAKNNAIKMIMIDQLPVAALTDNITYGEKSTTATLV
                     SPVVG"
     gene            10838..11602
                     /locus_tag="PLS35_00365"
     CDS             10838..11602
                     /locus_tag="PLS35_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNI17124.1"
                     /translation="MGTTQDTRERIIVPGPAGFHPPSAAQMGVSLPDPGQGLYYGLLE
                     PNEDTVIEEMARKMLTSPNATIFPGPLVLWAWNNHAVEKAKAVLEIAAQIPDVMIIPM
                     PDYRPKYPKIDPEEVINPNHPNLTIWGNKIEACIFIGVHCHYANLTLKMIRAGTNCCT
                     MAICAEQGHEDAMLTIRDSDTLKLKKTAQIFKKVREELGIKLPDNGENVRFTGTQSKV
                     HDGKTHTNPMTFMPSAAGAGSAAAFGHSPDQMKREG"
     gene            11751..12974
                     /locus_tag="PLS35_00370"
     CDS             11751..12974
                     /locus_tag="PLS35_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNI17125.1"
                     /translation="MTPEYMFLEAPRTKEFITGSEAAKEAIRRSNVDLAIAYPITPQS
                     ETMQLVGVLYGEGYVKEYYRGEEEVGVMAAIAGGSRAGVRCYTATAGPGTLRGLEGIA
                     SWPGHRLPVVAMFTCRVVNAPLAIQPDNIEVSYLLNCGMIVFHAENQQDMYDFTLAGF
                     VISEKNDVTLPVGVCCDGFFVTHARGYVRMQDRGMKLPPREPWRGAVPVLDAENPPAR
                     LSRDAPVQKSNFMAYNIHAVWQQEVWAAVERSRKYINQYMGGLLTAENVEDAEAIIIA
                     SGSAAAQSREAVRICAEKGMKIGLIKVRSLRPFPTQELRKLCGKAKLIVVPEFNYVGW
                     LAKEVATAIYGFSNAKIIGGPRVYGGQSMPVELIVDEVESGLTGKKSTNVAISQVMGA
                     SAADHEAMGHFMRSI"
     gene            complement(13059..13307)
                     /locus_tag="PLS35_00375"
     CDS             complement(13059..13307)
                     /locus_tag="PLS35_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17126.1"
                     /translation="MTDPILQAYREVEMAMERFTLVLHDHVDYLRKTEAPGSDKLHRM
                     ASGTKAMRDSASIYLSYAKYVAHGMPESPDLVEEDLQG"
     gene            13376..14581
                     /locus_tag="PLS35_00380"
     CDS             13376..14581
                     /locus_tag="PLS35_00380"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HNI17127.1"
                     /translation="MYQLSSAMLGTIATVARVNNSLQNPALSASLVSGVKNLSEMFTR
                     GTKAGATAYLEDGALRASYLHYYVPVNVGKIQMLLEELRPLLPSRTNEEFRVLDVGGG
                     PGTGSLALLDWCSSQSLPPISSLRMTVVDRSIEAMKLSTRLWEEYARQINIPSIQPLQ
                     TAVCDLEHSFPPVLRMGAEKNAYHLIIVQNVLSELFAEKSDCIARRTTLINHLVDCLA
                     KDGSLMLIEPASRAESRALHQVRDNVLSELQCSIYSPCLHDQLCPALLKPDDWCHEER
                     EWQTPSWISQIDREVGFIKDALKFSYVIIRKDGKSLVPRKQDYHRVVSELREMKGEKR
                     VWLCDQTGRSEVGRQDKERSTTNATFDNWHRGAIVRVDEIVRKERKGRPAMVGRILSS
                     GVAEVIRPA"
     gene            complement(14586..14771)
                     /locus_tag="PLS35_00385"
     CDS             complement(14586..14771)
                     /locus_tag="PLS35_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17128.1"
                     /translation="MTDTQKPKPPLPVADVAPKPRKEIPLVSVPNDRDMIAEEMAALF
                     DEDRVSHQHGSSEPEAD"
     gene            complement(14808..15167)
                     /locus_tag="PLS35_00390"
     CDS             complement(14808..15167)
                     /locus_tag="PLS35_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17129.1"
                     /translation="MALIQLEQVEKRLSSVQCAICKTNTFGIDRRTLQSDGECRGVCL
                     KCRYSFPVYTDMEFYLRTQPDIPYRLKEISCPNCQHRGVSLDFRITMSVREAIYFVTC
                     SACHTQFPERSSLEAFE"
     gene            15300..15722
                     /locus_tag="PLS35_00395"
     CDS             15300..15722
                     /locus_tag="PLS35_00395"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNI17130.1"
                     /translation="MINKRLLVGNADDAKNPPPQVGAILMVAEEQNVTVPARIIYAKI
                     PLKEFGEPAVSALYDAVQWIEKHLPDNRLMVCCRAGMGRSVSVVIAYLCCVEGMTYSD
                     ALKLALTRRPGGMPLPRLDKVIEDVFRRRRAVERPSLS"
     gene            15897..16742
                     /gene="mutM"
                     /locus_tag="PLS35_00400"
     CDS             15897..16742
                     /gene="mutM"
                     /locus_tag="PLS35_00400"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNI17131.1"
                     /translation="MPELPEAEVAVRQLRERVVGALVSDCWVGREDIVREGLSSLEWF
                     RGATITGVERRGKSVILAFSRAEEVKFMTAELGMTGLLLFRNTPTKHPQHTHFVLKLE
                     GGAESEIRYWNPRRFGRLSLLDQAGLDRYTSRRFGPDPLVISQKQFVQLLLSTRSRLK
                     PLLMHQQAIAGIGNIYANEMLFRARLHPDQIANQVNQKAAIRLHEIMGDVLREAIDLG
                     GSSVRDYFAPDGTEGQYKRRHLVYAKSGEPCPNDCGAVIRRSIGERSSFYCPRCQRIR
                     RPQVG"
     gene            16951..18150
                     /locus_tag="PLS35_00405"
     CDS             16951..18150
                     /locus_tag="PLS35_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNI17132.1"
                     /translation="MAKVLEGPGMGLMKKWGINVPNYVVVTSVDELTKLGQANEWLKK
                     SKLVAKAHEALGSRFKLGLVKVDLDFKAAEAATKEMIGRQVGSITVTQVIVSEMIPHK
                     EEYYCAVKSTREGSEILVANCGGIEVESNWERVKRLCLDIGQSPSPESLEKLSKEAGF
                     SGALAKKMAEFAGKMFACFDSEDAQYLEVNPVVLRAGDDELVALDAVTLLDGDAKFRH
                     PDWNFAFAAEFGRAYSKQELEVMAVDSKIKGSVKFIEIPGGDTAMLPAGGGASVYYSD
                     AVVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIRNIIVGGAIANFTDVKKTFGG
                     IINGFRKAKSEGKLKNVKIWVRRGGPREKEGLDAMRALKDEGFDINVFDRNTPLTDIV
                     DKALQAK"
     gene            18193..20013
                     /locus_tag="PLS35_00410"
     CDS             18193..20013
                     /locus_tag="PLS35_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNI17133.1"
                     /translation="MSILANKDTRVVIQGGQAGVNAARRMAEFCYLIKRPLNVEAFVY
                     PPDAGKTNEIPYGSGLIAIPVYKSIAEATKNHPTLNTSLVYIGADRAMKGGMEALDDS
                     HIKVVSMITEGVPEKDAKILGAHARKLGKVFNGPSSIGIISAGACRLGVIGGAFDNLV
                     LSKLYREGSFGVITKSGGLSNEIIWICSQFADGITTAIGIGGDAYPGTDYVSYLEMFE
                     NDPQTKAVVIVGEMGGDLEERAAEWYGAKKRRVKLIAVVSGFCQESLPKGMKFGHAGA
                     KEGMKGEGSARSKSDALKKAGALVPPTFGALGPAIKETYEDLLKSGQVKAPVEPAVLP
                     RLPKTVEEAMKADEVMVAPLIRTTISDDRGDEPCYDGYPASELINKGYEIPHVIGLLW
                     DKRLISKQEAEIIKRIMMLSADHGPCVSGAYATILAACAGIGLSQSVAAGMIMIGPRF
                     GGAVTDAGRFFKYAVDNKMSVDEFLAYMKKNHGPVPGIGHRVKSLRNPDKRVKELVGY
                     VKSLKIKTPCLDFALEVEKITSVKKDNLILNVDGTMAAVLVDIGFPVDSLNGFFILSR
                     TIGLIGHWVDQKRQDSRLIRLFDYLVNYAAPKRREVPPLK"
     gene            20121..22364
                     /locus_tag="PLS35_00415"
     CDS             20121..22364
                     /locus_tag="PLS35_00415"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNI17134.1"
                     /translation="MSMDLAKALYAKMPEVFAKARKKFGRGLTLAEKILVSHADNFDS
                     QNWERGKAMLALRPDRVAMQDATAQMAVLQFMQANKKKAAVPSTIHCDHLIRAEMGSE
                     KDLLRALDENKEVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGCLIIGTDSHT
                     PNGGGLGGLAIGVGGADAGEVMAGLPWEVLHPKLIGVRLTGKLNGWASPKDVILYLCG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTICNMGAELGATTSVFPFDEKMVAYMKITD
                     RADLATFTQSHKDLLVADPEVAQSPEKYYDQIVEIDLSKLEPHVVGPHTPDLARPISK
                     LKAEAQEKGYPVELKAALIGSCTNSSYEDISRSAHVAQQALKAGLKAKASFLISPGSE
                     RIYHTMKRDGFMDTFEKLGGTVLSNSCGPCIGQWKRSDGVKGRADSIVSSFNRNFPGR
                     NDGISETLSFLASPEVVTAYAITGDLGFDPVNQSVKGADGKEFKLQPPVGEELPAKGF
                     AKGEEGFVAPAEDGSNLTVDIPPTSERLQLLQPFPKWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANNSFSSEPGKGTNVLTGESNLTMAQIARAYKA
                     KGIGSIVVGDENYGEGSSREHAAMSPRFLNVRAVITKSFARIHETNLKKQGILPLTFA
                     DPKDYEKIEQNDRISVVDLANLAPGKPVTVVIHKTGGDLRIQTNHSMTAQQITWFKAG
                     SALNALN"
     gene            22390..24618
                     /locus_tag="PLS35_00420"
     CDS             22390..24618
                     /locus_tag="PLS35_00420"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNI17135.1"
                     /translation="MAKADKIIYTKTDEAPMLATYSFLPIINAFTKAAGVTVELRDIS
                     LAGRVIAVFPEYLTAEQKQHDALAELGEMAKTPEANIIKLPNISASIPQLVATIKELQ
                     SQGYKLPDYPENPKDDKEKDIKSRYDKVKGSAVNPVLREGNSDRRAPLSVKAYARKHP
                     HKMSPWSPDSKTHVSHMKGGDFRSNEKSMTIPAATTAKIEFVGADGKTTVLKEKVALQ
                     AGEVLDATFMSVKALRTFLEEQIEDAKKQGVLWSLHMKATMMKVSDPKIFGHGVTVYY
                     KDVFEKHAETFKKLGVDPDNGLGDVYAKIKSLPDDQRKAIEADIQAVYQKRPPMAMVN
                     SDKGITNLHVPSDIIIDASMPPVIRDSGKMWGPDGKLADVKCVIPDASYAPIYHEVVE
                     FCKQKGQFDPRTMGTIPNVGLMAQAAEEYGSHDKTFKAPANGTIRIVDANGTVLHQHQ
                     VEAGDIWRACQVKDAPIQDWVKLAVTRARATGAPAVFWLDKTRAHDAELIKKVNAYLP
                     KHDTNGLEIKIMSPAEACRFSIERMKEGKDTISVTGNVLRDYLTDLFPILEIGTSAKM
                     LSIVPLLNGGGLFETGAGGSAPKHVQQFQEEGYLRWDSLGEFLALAASLEHLAKVGNN
                     PVAKILADTLDQANAKFLESNKSPARKVGEIDNRGSHFYLALYWAQALAAQTADKKIA
                     ERFAKIAKDLSDNEKKIDGELLAAQGKPQDVGGYYHPNDAKAAKALRPSATLNAIIDA
                     IA"
     gene            24702..25412
                     /locus_tag="PLS35_00425"
     CDS             24702..25412
                     /locus_tag="PLS35_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNI17136.1"
                     /translation="MAQEDTNVIDQPEVLRHKMVTIEISGKKYEVPEGITVIKALWHT
                     GQEVVRGAGCLGGFCGACATYYRTKDDPKVRTCLACQMAVEDGMSFTIMPPFPARKAL
                     YDIQSLKDPKQDLFNLYPEAPLCRNCNACTEACPQKIDVREGVWKAVFGDFKSVSEMF
                     MDCVMCGMCTPVCIADIAPNLVALYVSRAQGAHFTEKPQGLDLRIKEIQDGRFNDEWN
                     KVLAMNEQELADHCATVK"
     gene            25481..27082
                     /locus_tag="PLS35_00430"
     CDS             25481..27082
                     /locus_tag="PLS35_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNI17137.1"
                     /translation="MDIHALQQIVHQTRDARRKQTIPKFSPADRDQLIHKYHPDYRAS
                     AYRPIRFGPNADDKTVVELAALLEGDSSIPEHLDLTPQYTADVLVIGGGGAGCAAALH
                     AHTSGAKVILATKLRLGDSNSVMAQGGMQISVAPEDSPVTHFLDTLKGGHMQNDHELL
                     KVMVEEGPSIAKWLIELGVLFDRQADGNLHVKKGGGSSKPRLLTCSDYTGLEIMRVLK
                     DEVLNQKIQLLEFCAAVELLSDDNGQCTGAIFKDLDNHRHVIVAAKSVILATGGIGRL
                     HIQGFPTSNHYGATGDGLCLSYRMGAKLDHIDTFQYHPSGAVYPEQLIGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGIRTMSGRIGVWLDTPLLDAEHG
                     PGTVEKHFPAMMMQFERFGIDISKDPVLIYPTLHYQNGGVKIDANSETNVKNLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRSGLTAASRAGSMSQGKLTLNHLKRFRAEAKKHG
                     NSSGVISPMILPAYTRKEPERTATK"
     gene            27168..28346
                     /gene="sucC"
                     /locus_tag="PLS35_00435"
     CDS             27168..28346
                     /gene="sucC"
                     /locus_tag="PLS35_00435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNI17138.1"
                     /translation="MNVHEFQAKQLFAQFGVPVPRGKEITSPEGATAWANELNTPVFV
                     VKAQIHAGGRGKAGGVKITKDRGAIAGLAKELIGKTLITHQTGPKGRTVHRLLLEEGA
                     NIAKELYLSILVDRDTGWPTFIASTEGGMEIEEVAEKTPEKIIKEAIDPAVGFQGHNG
                     RNVAFALGLQSMEPAVINPFVQMLGNLYRLFMEKHAALVEINPLIITKEKTLVALDGK
                     VSFDDNGIFKHEDVQKMRDLNEEDPLEIEATANNLNYVKLDGNIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKDTVAAGFRILLKDPNVKGIFINIFGGIVRCERIAHG
                     VIEAAKEVKITVPLVVRLQGTNADEGRKLLAESGLKLEVANDLWEAAQKIVKLTGKAA
                     "
     gene            28358..29230
                     /gene="sucD"
                     /locus_tag="PLS35_00440"
     CDS             28358..29230
                     /gene="sucD"
                     /locus_tag="PLS35_00440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNI17139.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHASQCKAYGTKMVAGVTPGKA
                     GQEVEGIPVFNTVADAVKKTDCDTSLIFVPPPFCADAILEAADAGVKLIICITEGIPV
                     NDMVKVKRALHGRDVRLIGPNCPGVITVDEAKIGIMPGFIHKRGVVGVVSRSGTLTYE
                     AVHQLSTLGLGETTCVGIGGDPVNGTGFVDVLPLFEKDPETQAVVMIGEIGGDAEEKA
                     AEYIKQHVKKPVISFIAGITAPPGRRMGHAGAIISGGKGTATEKMKALESAGVRVVKN
                     PAEIGHAVMKALGR"
     gene            29512..31146
                     /locus_tag="PLS35_00445"
     CDS             29512..31146
                     /locus_tag="PLS35_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI17140.1"
                     /translation="MLVFLIHVRDPQFYALPAKTRAKNGRIRVMGFPPIGIMSLSAVL
                     KRAGHECVMFDQAHPETPNEFIIEEIKRRKPALVGMSFLSTTSYPYAKILARQIRVAD
                     GSVKLAFGGVFASLNAGLVKLQCPEVDFVCRGDGEQLLLELLDHIDDPKDVAGVTWAK
                     DGKVINNPNRAMERHLDQWPFPDRESLELDFVESMPLDVPAVLSMERFTTMQTSRGCP
                     WPCVFCDIPIFNEGKWRARSAQHVVDELKFLEANGYGSVYFVDDHFLLQPKRIDAICN
                     GIIDAKLSIQWGIEGRVDSVAQHLFPAMAKAHCRTVMFGIESGSQKILDRLQKEQTLA
                     EVETAVRNAKKAGIEIVHGFFTVGNPDETVEDMEKTFDFASKLPLDTFGFNRLCVYRG
                     TPLWQEYIKRGLVSDAKDWYKYFKCSEIDPTCLPGATINSVRQEGLKKLFIYKLTRYP
                     IQTFKLLRRFLRYMPFRDVAYLIIKPFLGQKKGATKAEVLSRAVEHAEMKDAAAQLTQ
                     LSDELLHSVLEESKAERQRIQQEAESSRELPMISAR"
     gene            complement(31135..31872)
                     /locus_tag="PLS35_00450"
     CDS             complement(31135..31872)
                     /locus_tag="PLS35_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNI17141.1"
                     /translation="MATVRIGLSGYSYKPWQGPTRFYPPTLKQAQFLEYYSTRYRTVE
                     LDGVWFRLPTLSAVTKWIEQTPAHFVFSVKAHRTITHVKRLRPDAASFLHTMLEHLAP
                     LAEKQKLGPILLQLPPNFKRNDDRLAEFLQGLPPTYRWVMEFRHDSWNHATVENLLRR
                     FRVAWAAVETDDRPAEYRDTADFLYARLRRSQYSNKDLTRWGEYFLQTRRNGKDCYAY
                     CKHEDEGSPWMWADQLLKIMNVGTLPG"
     gene            complement(32204..33460)
                     /gene="ahcY"
                     /locus_tag="PLS35_00455"
     CDS             complement(32204..33460)
                     /gene="ahcY"
                     /locus_tag="PLS35_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNI17142.1"
                     /translation="MDYDVKDMGLADQGKLKIEWAEATMPVLRLIRKRFEKEQPFRGI
                     RATACLHVTTETANLMKTLKAGGADVRLCASNPLSTQDDVAAALVKHEGIPTFAVKGE
                     DNKTYYRHIESAIAHKPHLSMDDGADVVSHLHSKRKELLRHVIGGTEETTTGVIRLRS
                     MAEKKVLKFPVISVNDADTKHMFDNRYGTGQSTMDGIVRATNRLVCGSTLVVAGYGWC
                     GRGIATRARGMGANVIVTEIDPLKGLEAVMDGFRVMPMDEAAPLGDFFVTVTGNLKVI
                     RGEHFAAMKDGAIVCNSGHFNVELDIPALEKLSKKKLAVRSGVDQYTLKNGRRVSLLG
                     EGRLVNLATAEGHPSSVMDMSFANQALGAEFIVKNYKKLEKKVYPVPADIDKEISRLK
                     LAGMGVSIDKLTKEQVKYLASWEMGT"
     gene            complement(33567..34715)
                     /gene="metK"
                     /locus_tag="PLS35_00460"
     CDS             complement(33567..34715)
                     /gene="metK"
                     /locus_tag="PLS35_00460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNI17143.1"
                     /translation="MRHNYLFTSESVTEGHPDKIADQISDGILDAIIAQDKTSRVACE
                     TILTTGIAFVAGEISTKAYVEIPDIIRDVIKDVGYTDASWGFDSNTCSVLTSIHQQSS
                     DIAMGVDSGGAGDQGLMFGYATNETTELMPMPIVLAHRLTKRLAEVRKKKVLKWVRPD
                     GKSQVTVEYKDGKPFRIDTIVVSTQHSPDVTNKQIERDLMEYVIKPSMPKGLYDATSV
                     KHHINPTGRFVVGGPMGDTGLTGRKIIVDTYGGHGSHGGGAFSGKDPSKVDRSASYMA
                     RYIAKNIVAAGLASKCEVQLAYAIGVADPVSVLVDTKDTEKVAPESLDKLVRKHFPLT
                     PRGIIDHLKLRRPIFRKTAAYGHFGRNEPEFTWEKTDKAKALRKDAGL"
     gene            35113..36549
                     /locus_tag="PLS35_00465"
     CDS             35113..36549
                     /locus_tag="PLS35_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNI17144.1"
                     /translation="MNDKPDAASEMSASGPSTSGLGWEEFSREVVDFFASIKLAMFLF
                     IVLAMTATIGTVIQQGERPETYVQEYGEEAYRWFLRLGFTDVYHTWWFTGLLGLLCVN
                     SLTCFHKRFPSVWRSMRQDKVSVSLAFIQGMKQQTTLSLNQPKETVAESLVRLFAEKG
                     YKVLAKSDPGEVTVYATKGIMGRVGAHVAHLSATVIVLGGLLGSYYGFSEFGVCLEGQ
                     TYHIPRGNFDLRVDKFWIDYHENGSVKSYNSTLTVIDQGAPTMTKTITVNDPLVYKGI
                     WFYQSSYGDAWDQIEAARINIKDKASDKVVATVDLEWNKEKAVEGLPLKMKMTDFVAD
                     FAFNSTEKKVFSKTAEHSNPAIRLAVDERSTVQSTPWVFYHYPDLFEIKDSAYQFEFI
                     GYQPKKFTGLQIARNPGIMMVWIGCTMLVVGMTLSSLIYHRRLWARILPGESGVTVHL
                     GGTTHKSQIDFQKEFKKLTEKIQAKTSS"
     gene            36622..37536
                     /gene="ccsB"
                     /locus_tag="PLS35_00470"
     CDS             36622..37536
                     /gene="ccsB"
                     /locus_tag="PLS35_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNI17145.1"
                     /translation="MMRSLFLFDMTFWLYLAALGLYVAYLFAKRPAMQMAPAGHPIEN
                     LEDRDGAWATQLGQVATLVTVFGWVLNTMALMTRAFERMEHSGTFAPWSNQFEAMAYV
                     SWAIILGYVLLEFRYRIKAVGAFVVGIGFIAMGAASLLPYRYQTAEPLVPALNSYWIY
                     IHVSVTLTSYAAFAMAGGLGLMYLFKERAVNRGSQSRFYAAFPDLETIDELGYKAIML
                     GFPLLAFGIILGAMWANYAWGGYWSWDPKETWSLIVWLIYGAYIHARMTRGWEGHKAA
                     IYAIFGFLMVIFCFWGVNFLLSGLHAYA"
     gene            37540..38163
                     /locus_tag="PLS35_00475"
     CDS             37540..38163
                     /locus_tag="PLS35_00475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNI17146.1"
                     /translation="MSDILNQSSSGVTTRSGPSRTVVVLAAAAILGVMFLVVWLQSSK
                     YEPLIVGKEAPDFQLPDLNDKPLRLSDYRGKVVFLNFWATWCKPCREEMPSMEVLYKN
                     FEKDGLVILAVSIDRVTTKKEIPPFVKGLNLTFPVLVDSWGQTDKRYKLMGVPETYII
                     DQQGVLREKVIGPRDWTRLDNLKVLTQLLKPAEKAASAATVEAVPAL"
     gene            38183..38737
                     /locus_tag="PLS35_00480"
     CDS             38183..38737
                     /locus_tag="PLS35_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNI17147.1"
                     /translation="MMLSIIVGGLMIGGVMIADAPWASPLLSPAPAPILVASPAPQVD
                     EEAPNFLLKDSEGFNVTLDQFRGKVVLLNFWATWCGPCKIEMPAMERLYRSFSRKEFE
                     ILAVSTDAQGTAVTRPFQQEVGFTFPVLHDADFRIGLQYGARQLPMTFLIDRKGIIRN
                     RIPGARDWSAPAGKRLVEALLQGS"
     gene            38739..39485
                     /locus_tag="PLS35_00485"
     CDS             38739..39485
                     /locus_tag="PLS35_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNI17148.1"
                     /translation="MTDSVQSISLVAAFSAGLLSFVSPCVLPLVPSYISYITGLSIEQ
                     LTDVSERSRFRKAIILNSLLFIAGFSTVFVAFGASASLLGQVLITYQEHLRRFGGIVV
                     IVFGLYLLGILNLNFLKMEHRYQFRNRPAGFLGSFLIGIAFAAGWTPCVGPVLGTILL
                     YASTTESMFSGVVLLTFYSLGLALPLFLTALGVDRFLSYFKEVRSYLWGVSTVSGVLL
                     IVVGVMIYGNSLTMITSFLERYGIGWYLGQ"
     gene            39605..40810
                     /locus_tag="PLS35_00490"
     CDS             39605..40810
                     /locus_tag="PLS35_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNI17149.1"
                     /translation="MADGYGPTTYDVIIVGMGPAGSTAAAALSRGGLTVLGLDKDAHP
                     RYKVCGGGLSARIDHVLEPGFHSVVEQSVSGVQFVYRGQDPLLIESARPIAYMVMRDR
                     FDHYLVQEAIQAGAHIRTGHGVVELMQDQHGVEVVTAQGRYRAQIVVGADGANSVVAR
                     RFFPQRSQYRAPALESEVPLGNGRHGHSASTILVDVGAARQGYGWIFPKRACLSVGVG
                     EFRRKSKSLRATFDRFVTDEQHVIGGAVPRPAGHPIPVYTESEKREQDEESRFVAGRA
                     ALVGDAAHLVDPLFGEGIYYAIRSGQLAAHAILAHRDDHHMAVSAYAAALRREILPDF
                     RVTARMAQVIYAFPRLAFKLLGRYQDVVQDYFEVLQGRTTAQQFLPNAKKRLKASVND
                     LLLEALHLR"
     gene            complement(40836..41738)
                     /gene="bamD"
                     /locus_tag="PLS35_00495"
     CDS             complement(40836..41738)
                     /gene="bamD"
                     /locus_tag="PLS35_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNI17150.1"
                     /translation="MHRHSRDWCVRIAVMVGLLCFSAGCSSKPKTTAQGKPVSGTDEQ
                     IFLGDTIEKNYDPNVIMKRGEAFFDKEEFAEAIVEYQHFLELHRSHQLAVYAQFRLAE
                     SHLRMGKSIDRDPDPIQKAITAFEKLRKDYPNSKYEAQALQRLADCHDWLAKTHLFVG
                     QFYYRRSSYLAAAHRFDMIMKDYPDKTVAPEALYYLALTYQELGANDWAMEKLQLLAE
                     KYPNSDVAGSGRRLLAKLEKKSPPAAIVASKETPAPQSPPSNTLLAASVPSTFAAPAV
                     PELKRAPSAALSQPFVSCRLGAWC"
     gene            41940..42932
                     /locus_tag="PLS35_00500"
     CDS             41940..42932
                     /locus_tag="PLS35_00500"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNI17151.1"
                     /translation="MKCSRIVGTGSFVPPRVVHNEEVGKPLGLNSDQITALTGIRTRH
                     WAEPGQASSDLAVEAGRRACEAAGIPPQSLSAILVSTTSPDSAFPSTACHVQRALGAE
                     SVMAFDLSASCSGFLYGLSMADAMIRSGHIRSCLVVAAEVKSRSLDPADRETAVLFGD
                     GAGAVVLVQEESIRPQAAGILGVRLYAEGAGHGLITIPAGGSRKPSGTETVRGKDHLL
                     RMRGSAVFRAAIRHLEQAMLELLKEFGLSTVDVRRVIAHQANARILEQLRRRLGLPRE
                     VLYSVIERYGNTSSASLPIALDCAVREQRIAAGDVVVLAAFGGGLTWATGLVRW"
     gene            42957..43574
                     /locus_tag="PLS35_00505"
     CDS             42957..43574
                     /locus_tag="PLS35_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNI17152.1"
                     /translation="MFGLIPREEAFFDLFKNAAHNMIEGSRLLKDMTEDFRNPVEKAK
                     RIKDVEHVGDGITHDIARKLNQTFITPIDREDIHDLASALDDILDVVEAIADRFVLYK
                     VVAPTEAAIRLANVLYQAAVEVGATVDLLGKSHQAVTECSVRVNSLENEADRISRDAI
                     SSLFEKETDPIAVIKWKEIYENFEAGTDRCEDVANILERIALKHT"
     gene            43639..44631
                     /locus_tag="PLS35_00510"
     CDS             43639..44631
                     /locus_tag="PLS35_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNI17153.1"
                     /translation="MAELSGLLLVVVILALLFDFSNGWHDSANAIATVVSTRVVSPST
                     AVLVAGALNVAGAFMSTAVAKMVGTGIVDPQSVTQTVVASALAGAIVWNFMTLMLGLP
                     TSSSHALIGGLVGAALTHGGMAAVKFSGLRSVLEAMILSPFFGFAIGLVLMIVLSWLF
                     FRVPRALALRLFSRLQLVSASFMAFSHGANDAQKAMGIITLALVSAGALPKAEVPTWV
                     IGSCALAMGLGTAVGGWRIVRTLGMRIVKLEPVHGFAAETGAAIVLMATAHIGLPVST
                     THTITSTVMGVGAVKRVSAVRWGVTRRILSAWLFTLPGAALLSTICYLLLSKLS"
     gene            complement(44597..46456)
                     /locus_tag="PLS35_00515"
     CDS             complement(44597..46456)
                     /locus_tag="PLS35_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI17154.1"
                     /translation="MNLSLRWKVALGTLAAVLVGLLVAGWLVIRSVEQTELSRMAEML
                     ETRSSLAAMALRPLFDQTGQAVPSPLLHTTIRELSQQARLRITVIKQDGTVLSDSAVP
                     AEGLTHIDNHLARPEVAQALASGRGMDIRASQTTGERTYYVARLLSEPARIQPGVPVI
                     RLGLPLTSIDERVRHIQQDLLTAFGAAFLLAMVLSLWVSRNLTKPLSEMAAAARQLAA
                     GTPGIRLTVSSSDEVGLLARTLNQMTDQLETKIKEVSDDRAQLLAMLIAMVEGVMVLD
                     YRGTVVQVNPALERMFALELTESRGRHYAELIRHEGLTALVSAVLQTRSGQGGEITLS
                     PSGSCLRVEASIAGGNREQEACAVFVFHDITELRRLEKIRKDFVANVSHELRTPLTSI
                     KGYVEALLDGGKDDPSTAAAFLEIIMRQSNRLNLILDDLLQLSQIESGQVLFRREPVE
                     LRALLERTVAVIKPLADKKHHTIELSLPDEYVVVEGDEERLVQVFINLLENAVKYTPD
                     QGRISMAIRQATQSRTASPRPMIEIVVADSGIGIPEADRPRVFERFYRVDKARSRELG
                     GTGLGLAIVKHIVEAHSGHVWVEGNAPRGSRFVVHLPVAQESSTTISTAAGNI"
     gene            complement(46453..47169)
                     /locus_tag="PLS35_00520"
     CDS             complement(46453..47169)
                     /locus_tag="PLS35_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17155.1"
                     /translation="MSSPSSTHKKILIVEDEKDILQLVKLYLEKEGFRTVSAMTGTEG
                     LRQVKAEHPDLIILDLMLPELDGLEVCKRIRLNQETALLPILMLTAKAEESDTVIGLE
                     LGADDYVTKPFSPKALVARVKALLRRLDRNANSPQTQYHYGPLTVDLSRHEVRLEGEE
                     VTLTAKEFGLLEHLLRNVGRVLTRDVLLSAVWGYDYYGTTRTVDVHVRRLKQKLPLLD
                     DAIISIKSLGYKLRESDIPA"
     gene            47344..48306
                     /gene="ispH"
                     /locus_tag="PLS35_00525"
                     /gene_synonym="lytB"
     CDS             47344..48306
                     /gene="ispH"
                     /locus_tag="PLS35_00525"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNI17156.1"
                     /translation="MKIYLANPRGFCAGVDRAIDIVDLSLKKYGAPIYVRHEIVHSRH
                     VVNSLRHKGAVFVEELNEVPEGSVVIFSAHGVAKSVWEEAQSRRLHVIDATCPLVIKV
                     HNEVNRDYTQGYELILIGHAGHPEVIGTLGQIPDKFHLVSSVQDVETLHVEKTQNLSY
                     VTQTTLSVDECRDIVEALHRRFPNIKGPHQEDICYATQNRQNAVKALSKLVDVILVIG
                     SPNSSNSNRLRELGEHCGIPSYLIDAASDINPDWLKDAKSVGLSAGASAPEVLVTEVV
                     AYLKRLGSSEEVEELTVIEEDVEFLLPKELVTIESASRTAASAN"
     gene            48497..52180
                     /gene="smc"
                     /locus_tag="PLS35_00530"
     CDS             48497..52180
                     /gene="smc"
                     /locus_tag="PLS35_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNI17157.1"
                     /translation="MYLKSLEMLGFKSFAEAKIQFPKGITAIVGPNGSGKSNVVDSIL
                     WVLGEQSTKTLRSEKMEDVIFNGTEVRKPLGLVEVSLVIGGLGELRLDAISGLPSQLS
                     EYQELMITRRLYRNGDSEYLINKTPCRLKDIRNVLIETRAGSKGHTVIEQGRIEQILQ
                     ASPQDRRELIEETAGIVRYKKQKAEALRKLESTQQNLLRVRDIVAEVKKQLNSLERQA
                     RQARTYQTLQQEAKGLEIELLSRDYRTMHADLESVDREAREVEALEAEQVAEQARLDS
                     EQEAIRLRMNDTAEAISRVRDTLAGTEQRQSQALTAAEVERNRTELFERQRVQASQEM
                     ERLAGDQEQAQVEIEDLRATLLQLEGDIVEQEAQFQQADAEAKALAGHRAAAVSEEER
                     ARKDVLNLAVLVANTEQSLAQVTARQQETSARAERVSREQEQMVAQVAGLDEQRELLA
                     AQREEASQRIQGLMAERQAAIERIEGLGGQITGLDRDIVRFSEDVAAVESRLGALQGV
                     VREEMGYGREGEEEGTALKTCDGVREALAEWLVIPSGLDRAVETILGERVRGWLVDQP
                     SAASRAVEFLKGKELGRGAFIPQQIRWATDQAAADSSASWWPALNGQPGVVGRAIDVI
                     RAEGASASTLSYLFDGIVIVESLAVALQLWEQHQWPAPAGPTYVTLSGETLDAAGVMT
                     GGGTSAGAGLLQRRREVLELEARRMEAVQALELARVAREEAASALGLGREEEQQLGRA
                     IREAEMLELSLQKDDANVERQRGELHRRMDMLAEELQRGLAEQARLQEQLESSQAQLE
                     QWVAEKLGQENGLLQIRERLVVIESQGLAIQQRLTEVRLSLESMRGRREHAHSDIARF
                     TQRLDAAQRRAAELEEQVTGLVQATESSREEQTRQEALCRELGAEVDGIKSELIAAQE
                     RQAEEMSGLHTVEETLSTVRQSLSALHDRRMTAEVRKAEVKAHLSTIESTLAGTYQID
                     PATLLVVSTEEQPVTEGEEPAAPVSLLETPQLREQIQKIRERLDRMGAINLAAIDEHR
                     ELEERYQFLTTQEQDLSTSIASLKEIIQKINRTTKDMFVETFNELQQKFRDVFAQFFP
                     GGRAELQLVEEPLEEGVEDNGAREPGVEIVAQPPGKRLKSITMLSGGEKTLTAMALLI
                     ASFLIRPTPFCILDEIDAPLDEENIGRFTSVLRSLSSTAQFMVITHNKRTMAMADSLF
                     GVTMEEPGVSTLISVKLGDLQPA"
     gene            52404..54575
                     /gene="shc"
                     /locus_tag="PLS35_00535"
     CDS             52404..54575
                     /gene="shc"
                     /locus_tag="PLS35_00535"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNI17158.1"
                     /translation="MRLLKNLFSRLSDSLLVSVPSRIRAARDFASAPVLRLVSSKPDA
                     PASDGAKRAPSHTTGQLEALEEAIRKSQAWFLARQDATEGFWVAELEADTTLSSEYLM
                     LRRFMNCVDPERERKVIRYLKSAQLPCGGWPIYHGGPADISASVKAYFALKLSGVSAD
                     EPFMAQARTCILDKGGVVAANVFTKIALALFGQYDWRGVPSMPPEIMLLPKRFYFSIY
                     AISYWSRAVLIPLLIIFAKRPQCYVPPEQGIDELYTQPPAEIDYSTVPPFKKDRTWFT
                     ARNFFINLDALLKVYDRSPVEWVRQKALKGAENWMLDHMKGSGGLGAIYPAMANSVMA
                     LNCLGYKNDDPLVVKAMREIEELEIHDTVMDNGQCVDALHLQPCHSPIWDTALLINAL
                     SEAGMSQDHPALQKAATWLLSKQTKTVGDWIISSPGAEPGGWYFQFENELFPDVDDSA
                     VVLMALAKVRLPDEDQQRLAIRRGCRWVTAMQGSDGGWGAYDVDNNRIVFNYIPFADH
                     RALLDPSTADLAGRCLEMLATLGYDWTHPSVASALTFVKNDQEQDGSWYGRWGVNYIY
                     GTWSVLSGLRAIGEDLSSPYIRRAVSWVESKQNPDGGWGESCLSYADVAESGRGDSTP
                     SQTAWALLALMAGGVTDSFSLARGIHYLIRNQRKDGSWEEVRHTGTGFPRVFYLRYHW
                     YCQYFPLWALAMYRNLKTRGTTRADELRQLAYQSGQFRSPR"
     gene            54630..55385
                     /locus_tag="PLS35_00540"
     CDS             54630..55385
                     /locus_tag="PLS35_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17159.1"
                     /translation="MTTIGVFVATRWELAAVRHAFPASEVRAVGGIRCVVVRQPFVEW
                     CIIPMGVGPERAMATAKRILAEQALSAIWSTGFACALGPAGIGDVLIGTDVILENGGK
                     AGPSVPCAPAMVEWVRQAVHVQGLPMRSGGFVTVPRVLCLAEEKRAIAGRAGGIGLDM
                     ESAALGRVASEHRIPFAIVRTVSDLEDESLPLDFNLFLRPSGWAKGVVACLAHPTSLI
                     GLNRLRVQSQIAGAQLTALFRACADRALGEGIG"
     gene            55440..56207
                     /locus_tag="PLS35_00545"
     CDS             55440..56207
                     /locus_tag="PLS35_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17160.1"
                     /translation="MEGIARIGRFTIDLTEQMGRMMLFVLSSFAWLTRPPFRFYQIVK
                     QLNFIGYKSTFVVVLTAVFTGMVLALQGYYTLRKFGSEAVLGSAVALSIIRELGPVLA
                     ALMVTARAGSAMTAEIGIMRITEQIDALDTMAINPLQYLIGPKLVASLIAVPLLVALF
                     DVVGIYGGYVVGVQLLNGNEGAYWSSIESAVEWKDVYGGILKSISFGLLISWVCCYKG
                     FHTKHSAEGLGTATTEAVVLSAVLILVWDYFLTSVLL"
     gene            56292..57053
                     /locus_tag="PLS35_00550"
     CDS             56292..57053
                     /locus_tag="PLS35_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17161.1"
                     /translation="MIKLVGVEKTLGKQAVLRGVDLTIPTGKLTTIIGRSGEGKSVLL
                     KHIIGLMQPDRGEVWIDGTDIARLKGRALNEVRKKFAMLFQGAALFDSMTVFENVAFP
                     LREKLRLKGDVVTRRVEEKLEQVGLKGMGHKFPAELSGGMRKRAGLARALVMEPEIIL
                     FDEPTTGLDPLMAKAIHDLIVAMQQQFRFTAVMVSHEIPEIFGISDYVAMLKNGRIAE
                     MAPSSEFVKTTDEEIREFIFVGGTVAPKGLPAESR"
     gene            57065..57508
                     /gene="mlaD"
                     /locus_tag="PLS35_00555"
     CDS             57065..57508
                     /gene="mlaD"
                     /locus_tag="PLS35_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNI17162.1"
                     /translation="MERAKLELMVGIFVLVGIACLGYLSIKLGKLEVIGGHNYPIEAE
                     FTSASGLKPGASVEIAGVEVGRVRTIGLNSDRALVALAIQDGVKLYSDTIASIKTRGI
                     IGDKYLALSVGGGGDPLKPGDKIRDTESGLDLEELVSQYVHGKVN"
     gene            57595..58209
                     /locus_tag="PLS35_00560"
     CDS             57595..58209
                     /locus_tag="PLS35_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI17163.1"
                     /translation="MHVRRWMVVGVLLALQLFVGGLSFALAGPATESIRGTVDEVLRI
                     LNDKELKAPAKHEDRRQRLEKVVAARFDYSEMSRRSLGAQWNQLSDKEKQEFVDLFRT
                     LLTNTYADRVETYSGEGVQYLNERTEKEYAEVRTKVLSGKTEIPMDYRLLNKSNDWHV
                     YDVVVDGVSLVNNYRGQFTKILHTSSYSDLVDQLRKKSEKIKAP"
     gene            58243..59448
                     /locus_tag="PLS35_00565"
     CDS             58243..59448
                     /locus_tag="PLS35_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNI17164.1"
                     /translation="MQRHYFSSIRILLALAGLMFLSSPAPAGADTQIFPVPSVSTSKN
                     DGNDAGLIAPILISDPDGELKYLMAPMLIQNSIVGSRAVFNLFKYDPGGRQMKLIASL
                     TEKIERKVLFDYVDPAFGNGQYFLNFGGTFFKNATSRFFGLGQSTVQADESNYTAREA
                     RAYWRLGLYANEVTQVSVGQRVRQVQLQRGATDLPFSVEQFPTVDGIQGESIIVGHRA
                     SFYYDTRDSLVTPTDGMSVMAYAELNQNVKNGDHPVYSRYEIEVKKLFPSESKRAILV
                     VRADLQATIGSQVPFFEQSSLGGQNNLRGFGMDRYIDKHLIAFSIEERIHILRTKLAG
                     VTADFELAPFLDTGQVFNSFKDVSFQDYRMTPGVGFRAIVRPNVVGRLDYGYSREGGA
                     IFAGLDFPY"
     gene            59478..60476
                     /locus_tag="PLS35_00570"
     CDS             59478..60476
                     /locus_tag="PLS35_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HNI17165.1"
                     /translation="MRHLVYFFCLIGLSCGLPLLAGAEGFGPFPVRNFQALDQLVLAM
                     PGERAAVLRKGDLDIRLEAANTATIARDREEQADVTMKFETVRAGLFLRYGLTERLEV
                     GAEIPAYHRYSGFMEGAILGVERGTTGVSPPRKALRDTGYAFNVSNGGRTMFQGSENA
                     TGLGDVSFYGKYQLLQETGSQPALSIRMAVKAPTGDTGQVFGSGHPDAGIGLALDKTF
                     ATGWILYANVNGIFPTGRIAGLDVQPVVSGLVAVEYLWTENFSITAQFDYYSPPFHGT
                     GTRVLDKGVTESVLGVSYRILPRLLWQVYGVENLDFITGSAADFTLSTVLTYRFRS"
     gene            60657..62600
                     /gene="dxs"
                     /locus_tag="PLS35_00575"
     CDS             60657..62600
                     /gene="dxs"
                     /locus_tag="PLS35_00575"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNI17166.1"
                     /translation="MSLLKNIHSPADLKRLSPEQFPELCQEIREQILSVVSNVGGHLA
                     SNLGVVELTVALQYLLDTPKDKIVWDTSNQAYTHKLLTGRREQFHTLRQYGGLSGFCK
                     REESAYDTFNAGHAGTGVSAAFGMVEAREQRGEKHKVVCVVGDGAMTAGMTLEGLHHA
                     GGTNKDFIVVLNDNQMSISRNVGAISAYLNRTFTGEFYARMREETGQLLRKIPHIGAE
                     MQKIARRAEELAKGAILPGLLFEELGFQYAGPIDGHNFEHLLPTLENVLKMKGPVLLH
                     VITKKGLGYQAAMDNPVWFHACPPFVRETGVPAKKAVRPSYTSMAVDALIKVARQDKR
                     VVAITAAMCEGTGLNAFEKEFPERIYDVGIAEQHAVTFAAGMAAQGMKPVVALYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVVAAPKDE
                     NELQHMIKTCVSFDGPASVRYARGVSLGVPMDPEPTTLPIGKGELLREGTDVAIVAIG
                     VTVWPAMKAAERLAQEGISAAVVNARFAKPLDTELILKTAKNVRCLVTVEEGCKMGGF
                     GSAVLETLSEAGVMLRTKILGLPDWYIEQGPQDLLRERYGLTADGIYNSVKQLFGAGV
                     VADDAARLASLVGSLPHGDEQGS"
     gene            62615..63814
                     /gene="ispG"
                     /locus_tag="PLS35_00580"
                     /gene_synonym="gcpE"
     CDS             62615..63814
                     /gene="ispG"
                     /locus_tag="PLS35_00580"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNI17167.1"
                     /translation="MHITRKKTRQIQAGSVKIGGDAPISVQSMCSTDTRDVKATVEQI
                     RQLETAGCELIRVAVPDDEAANALPKIKAAMSVPLIADIHFDHRLALKAAEVVDCVRI
                     NPGNIGAWWKVQDVIKAVNERGIPLRVGVNGGSLERPLLDKYGWPSPEALSESALNAV
                     HALEDEGFTNMKVSLKASDVHHAIDAYYLFSTQSNYPLHIGITEAGTAMTGAVKSAIG
                     LGWLLSQGIGDTLRVSLAADPVEEVKVGFEILKSLELRHRGINVIACPTCGRVEIDVV
                     RMANELEKKLGHIKTPLNVSVLGCVVNGIGEGKEADIGIAGGEGKGILFKKGKLMRKV
                     PMDELMDTLIHEVEQLAKEKEAEASGVTHNGSSDGQTEEGWELMGHAPDEQSTIVRDI
                     PVLPSKP"
     gene            63916..63991
                     /locus_tag="PLS35_00585"
     tRNA            63916..63991
                     /locus_tag="PLS35_00585"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63949..63951,aa:Cys,seq:gca)
     gene            64449..66149
                     /locus_tag="PLS35_00590"
     CDS             64449..66149
                     /locus_tag="PLS35_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNI17168.1"
                     /translation="MTPDPIFFRDLAYVFIAAVVGGTSAYLLRQPLILGYVFGGILIS
                     PLTPGPSISDVHSFELFAEIGVILLMFSIGIEFSLNDLLRVRWVALLGGPLGIFLSIL
                     LSVGLGHLVGWTMIQSVVIGSVVSVASTMVLARLLLDSGDLRAKHGRVMIGITLVEDL
                     AVVVLTVLLPALGTLEPGRFATIGKALWLAVLILVPFSYLAAKAIPPLLTRIAKTQNA
                     ELFLLVSLSLGIGTAAVTQMAGLSLALGAFLAGLIISGSEYGHETLARLLSLRDAFVA
                     LFFVTIGILIDPRIVWSNLPLLGLMLGLILIGKFVIWTLVVRLFRYAWGTALLVGVGL
                     TQIGEFSFVLVQVAKTAGHVGEDVYNATLAASLLSILLNAPFVRYAPRWVAALHLRMG
                     RAAMPMQGGVQDEQAGHVVVCGFGRMGSTVGLALDHFGIAYTVIERDPDLVRQLRRRG
                     ISCVYGDASQTELLTAAGAERAALVIVALPVIHEASLTVRRLRGLNEKIPVLVRAHGF
                     REAEDLRAVGATEIILPEIEGAHTLIRHAFQALKVPKPDILAYIKSGQHFRASDADGE
                     "
     gene            complement(66238..66861)
                     /locus_tag="PLS35_00595"
     CDS             complement(66238..66861)
                     /locus_tag="PLS35_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI17169.1"
                     /translation="MRRSSIGLLFLALLCIPAVTRAENYVAAGIGVNAPSFDTTDAGK
                     VSLQKDLFYGGKIGHYFNDRGYNWFGLEVDMYRSTPGIKQQTLPTSSNPRLSGPMPGA
                     DLQVHSLAFNALVRVTGYQYKVEPYAGLGIGLNVGNISNGNFRPEASFAPSFNVLAGI
                     RYYMTESIAPFIEYKYNFAQFKFDRSNVTADYRANLFMFGIAYHFGR"
     gene            complement(66953..69226)
                     /locus_tag="PLS35_00600"
     CDS             complement(66953..69226)
                     /locus_tag="PLS35_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17170.1"
                     /translation="MKPLLSHRFPISPLWSGLLLALLYLNVHDVLRAEESASPTTSHV
                     VDMRFSTTTFLLNLEKRGLEDNTFTNGLGRDTTLIGNLINATLYKRLLPSLEAEIGVF
                     ANMPFGHDHEISQVRPVVRLTYQPVEQITAVAGTIRTPHRDFHDAVFDNGNRFLRPIE
                     QGAQMLVDSTHYQQDLFINWEQAFRGSAPNRYDVGYAGQLKFGPLHFNGQAHWVRNGQ
                     ALFKLDRSFSTRDNLVVATGPELVLEPSRWFASTAWWHQIGISFTYLTSYNQADNGSD
                     ASRGRGYELRTWLDLAGWRPYLAFWKGRNFLSQQGDPEFRASNFPEFGLSKTIRLGKR
                     ASIEFGAQARRIRAFFTEDGLNYMNRDTFKWVNQEYLVFNWDWDTNHADLIGDVWASL
                     SRPFDDRQDAPSERRFTAKLDTLTYVYSISYHGVRQIGGIPVSNHTFAGQYLSPVLQY
                     RVSPQLTLDAGLFAGLPVGDTQSFHTVQPILSAQYEMVPDVRLVAGTLHRNHPFVDAL
                     FNDATLFSRPVEQGFQLLVDREHYQQDLFINWNQIETYQKAERFDVGYAGRVAAGPFA
                     LNGQVYWTHFGGAQYSEARTFFGPGLPRDRPAGNNFLTAFGPQFTWQPKQYWPQLSWF
                     REIDVMALYLTSQNEPTQSSSPIVRGRGYQLTAGVNLDGWRPYITLWRGEHFVTERGD
                     PAYYAGDFTEFGLFKDFILPAGFSLRLGGFGRTIERHLTHTEYALLNWSWDQTPWRGF
                     CQRPTLLHRSESSCGQS"
BASE COUNT        14941 a        17978 c        20664 g        15688 t
ORIGIN      
        1 ggcgggtcgt tgtgccatcg gcctgcgaaa aggcctcgaa gatgcgctga tgggcttcgg
       61 cggggatgcc gacgcccgaa tccttcacgg caaaccgcag ggtgacagag gtgtcagtct
      121 cgaccacggc gtcaatacgc acgttgattt cgcccgttga ggtgaacttc agcgcattac
      181 tgatcaggtt cagcaacgcc tgccgcagcc tgatgggatc gccacgaagg gtgcgcggta
      241 tcgaactcgg catgtggcac gtgagttcca gccctttgcg ttgggccggg gtggcaaaga
      301 gttccacggc gttatcgacg gtgtcctgga gatcaaagtc cagccgttcc agctgcagtt
      361 tgcctgcttc gattttggag aagtcgagga tgtcgttgat gatcgccaaa agggcttcgc
      421 ctgattgctg gatcgtgcga gtcaggtggc gttgccgggt gtccagtgcg gtggtcagca
      481 ggagttcggt catgccgagg acgccgttca tgggtgtccg gatctcatgg ctcatgttgg
      541 cgaggaattg tgacttcgtg cggctggcgg cctccgcagc ttctttcgcc agcaggagtt
      601 cctgggcggt gcgtttgagg ggagtaatgt cggaggcgac ggccaggtac cccgtgatct
      661 tgctgtcggc gtcacgcagc gccgtgatat tcaacagcac cgttcgcctg gtcccgtctt
      721 tcctgatgta ggtccactca tgttcattgg gaagattgcg ccgggatttt tcaataaaga
      781 cgtcgaaccc cggtgcaatg gtgcagccga attcctcgga gagttgtctg gcccgctcca
      841 cgatttcctc gcgaacgtgg aagatccctg gatgttgacg tccgatcagt tcgtcggcgg
      901 tgtaacccag gagcgcttct gccgccggat tgaacaattg aatgatgccg tcaggctggc
      961 aggaaataat cgccgagccg gcatggtcca ggatggctcg ttgaaaagtg gcataggcgc
     1021 gtagttcggt ttcagcgcgg cgttgttcgg taatatcgcg aacggtggcc tgcaatccct
     1081 cgacaccttg gagtgtgata cgagtcaatt ggacagtggc caaaaaggtt ggtccgccaa
     1141 cccgtttgtg catccattcg aagagatgaa acccctcccg catggcagcc tcaatggcct
     1201 tgcgcgcctt gaccatggac aactcgccgt ccggctggta ttcgggtgac acatctcctg
     1261 ggccgaggga cgtgaagtgc gcctcgtccc gggccccgaa cagttggacc gtggccgggt
     1321 tgcaggcaat aaaattccag ttgggcgggt acagaatcat gatcgcatcg cgcgaagact
     1381 cgaagaggcg ccggaatttc tcttcgctct cacgcaggct cgactcgtac tgttgccggg
     1441 cgatgacctg ttcgctctcg agaggagcgt cgggagcttc tcccggctgc ggagaatgca
     1501 acaatcgttg gagcatccgg cggatggtca tgacgcctgc tccctcatgc tgccgaccgt
     1561 ccttccccgt tgagttcatc ccgcatcaag gcagagactg cgcgatgctc aggaatcaag
     1621 cggtcataca gttcctggaa gtccccgatc cgtcctgcgc gtgaggcgga ttcaagttcg
     1681 gtatacagga gagcaaggcg ttgtgccccc agcttccctg aggaggattt catcgtgtgg
     1741 gcgatcgtgg cgatggctcg agcatcctgg tcggcgatgg cgttctctag ccgagcgatc
     1801 tgcttgtcgg cataggggag atagagcgaa agggctttat gtaaaaagtc cggctggcct
     1861 ggtcgctggc gggctcgaat ggcttcccag gcatgcgcgt ccacatgctc gctctctgct
     1921 gccaagggct gagcatgaga tgaaacggcc tgttgaggtg gaaccccgtt cgatacttcg
     1981 gccggctgcg agtctgaagt ggagtcggaa gggacatgtt cgggaagcca gcgttggagc
     2041 agctccttca gcgcgtccaa ggtaaagggc ttggtcaggt aatcgtccat tcctgcggca
     2101 aggcagcgct cacggtcccc ctccaatgca ttggcggtga gcgcgacaat gatcgtgtgt
     2161 cgggccggca tggattgctg ttcatgggcg cggatcagcc ccgtggccgt caggccatcc
     2221 atttccggca tgtgacaatc catgagaatc aaatcaaatg ccccatgcgc cgccgtctcg
     2281 accgcctctt tgccctgctc cgccacggcg gtcgtgcatc ccaggatctc gagcattgtg
     2341 cacgcgacct cgcggttcac aggattgtct tctgccagga ggatatgccc ggccaatgtt
     2401 ctcgtctgtt cgggaggctg aatggttgca ggagcgtctt cagcggacgg cgcaaggcaa
     2461 aggcgcgtga gacaatcttt cagcaatgac tgatgaacag gttttctcaa ccacgcattg
     2521 aacagcttgg gatggtggtg agattcgtcc gtctcgcgat cctctgagct cagggcgagg
     2581 atcgccatgt cgcgcagatc tacgtcttcc cgtattttcc gggcaaggtc gatgccgttc
     2641 atgccaggga ggcgaatgtc cagcaccgcc atctggatct tctgattccc gcctttggcc
     2701 tcggtgagca ggcgcagccc atcttcgctc gaggcggcgg attgcaccgt cgctccccag
     2761 ttggataagt gtgcctcaag gatgtgccgg ttagtggaat tgtcgtcgac gaccaggatc
     2821 gagtggcccg ccagcggtga cgccaggcct tcatgggcgg agacagcttg tgaggcgtat
     2881 tccatcgaga cggtaaacca gaatgtggat ccttggccgg gaatactatc gacgccgacg
     2941 tcgcctccca tcaagtgggc gagttgtttg acgatcgcca gcccaagacc tgtgcctccg
     3001 aagcgccgcg tcgtggaccc gtccgcttgt gagaatgctt gaaagagtcg cgccttggcc
     3061 tcgtcggaaa tccctacccc cgtatcccgg atgcagcatt tgaggttcac gcgggaggct
     3121 tcctgagaaa ccaggctgag cgtgaccgat acgtcgccgg cctgagtaaa tttaatggca
     3181 ttgccgacga ggttgagcag aatctggcgg agccggaccg gatcgcccac gacggcatcc
     3241 ggaatctcct cggggataaa gcaggtcagt tcgagatgtt tccggtgggc tgtttctgcc
     3301 actagatcaa tcgcctcctc aaccgtctgc cgcaagccga attcaatgca ttccagtttc
     3361 aatttgccgg cctcaatttt ggagaaatcg aggatttcgt tgatgatgtc caggaggccg
     3421 gtcccggact ggtgaagcgt tttcgcgagg gagcgttgct tcggcgtgag ggcggtcgtg
     3481 agcagcaact ccgtcatgcc taagacgccg ttcatcggcg tccggatctc atggctcatg
     3541 ttggccagga attgtgactt ggcttgattg gccgtttctg ccgcgtcctt cgcttgatgt
     3601 aattcggtgg tgcgctgccg cacggcgtct tccaacgagt cagtgaagga ctgtagccgt
     3661 tgcgcttggg tcctaagcag ggcggtggtc gtttccacca gatcgaccat ctgctccagt
     3721 tcgcctgtat aggacgcggg gccaggcgag tcgccgttaa gcgacgttgc gtttcctgcg
     3781 tcttctttca aggggcggtg caacgtatgg agcgtatcct gcagtttcgc ggccgtgtct
     3841 cgaaagaggg cagacattcg cctgatgccg atttgtccga ccacaatcgt gacgctcatc
     3901 agcagaacgg aaagcactaa cagacgacga acttcgcgga ccaactcgcc ttgcattgcc
     3961 gcaagagaaa attcgatgcg aacccacgcg gcgatgtcgt cttcattctt gagcggggtg
     4021 acgacgatca gcgtcggggt tccgtcctca ctcagtcggt gggccattgt ctccgaccct
     4081 gaggtttgag cgagagagag ggtgtgatcg gtcagttccc ggccgatatc gtgaggcttt
     4141 gtcgcggcga caaccatatt gtcgggatcg atgatgacga tgccgacaat gtccgcctcg
     4201 ttggcatatc gggtgagagc ctcttgaatg cgaaacaaat tgtccagaac ggcggccgct
     4261 cccatcatgg cgaaggtgcg gctgaccgcc agtgcgcggg cttgcgcggc gtctaccagg
     4321 gagtgacgga tttctaccag tcggatggtg gcgaacaccg tcatgccaag ggtgatcagg
     4381 accgccatcc ccacactcag ccggagccgg agactccctt gccacatcat ccagaggtga
     4441 tgaaaccagg gcatcttatt tcttgttgga ttcagcatga gcggcgaagt caaaggcgat
     4501 ggtcgtctct gcctcagcgg aaacacgcac agtttgttga tagcttccca gcgtgtgatg
     4561 ccagatattc accacatgct cacccggtgg gaggtttgtg atctgaaatg aaccactttc
     4621 atctgtctgg ctgaaaaatg gatgcgcaaa ggcgatcaca tccgcatgca tgaatttgtg
     4681 tgcgtcgcat ttgaccgtat agatgccggg ttgcttgaga ggctttcgta ccggtcggct
     4741 ggccgggatc atcgccacat tcacaaaggt gcgtgatccc agggagatgt gcgtgttgtg
     4801 catgaccggg tcgtcgctgc ggatctccag ttgttggcct acctgtccta tgaccgtgcg
     4861 tggcaggaat gcgcagtggg tattcgtcag gacgagcgga gcaagagggg catcgcttga
     4921 gggaaggttg ccgctctcga tgctcacgac gacgccttca aggccgcgcg tctttggatg
     4981 gatcacgacg tctttgatcg tggtgcttgt tccgcaaaat gcagaatcgc gagtgatctc
     5041 ggccgtctgg tcaggcaggg ccgacccgct ataggtaatc ctgccggcca acttgccggt
     5101 cgggctttcg gcctgcagca aagagggaac tcccagggtc aaccccagga ctaccagcat
     5161 gagcctcatc accatgagtg gccgttcctt tcttcgccgg ctgtgcggtg cgcgtaaggc
     5221 gttgcgcttg atggcgtgat gatcatcggc gtgactcatt tctccggacg gcctgaaagg
     5281 gattggaggt accacaccaa atcccaaacc gcctcttcct gtccggcaaa ctcgttcgca
     5341 tacgagggca ttggcgtgcc gtcgaggcca gtcatgatgg atcgatagag gtcttgtgcc
     5401 gtgctgccgt tcttgaatga agccggatca gtgagattcg tggcgcgtat gggcagattg
     5461 ttggtgtcac gaaggcgtcc ggcctgcgat tccggtccat ttccttctcc gtttgctcca
     5521 tggcagaccc gacagtcgta ctttgcgtag agcactcgcc catgctgtac cgcgccctcg
     5581 ggtcgtgaga ccgggggagg aggtagagga atggatacgg gtgtttcctg ctcgaaggca
     5641 gggttgaatg atttgagata ggcaatgacc tgccatcgtt ctgactcgtt aagggagcga
     5701 taggatggca tgacccctgg aattccttgc gtgagagtcc tgaacaggtc ctgatctgtg
     5761 ggcaattcgc cggacggtgt gctgcgtagt ttgtagcttt cttttgtgaa gtcgcgtgga
     5821 cgaggggcgg agtaacggcc gatggaccaa ccggatggtc catctcccaa gcctccgttc
     5881 ccatggcacc acgcacacgc tcgctcatag atccggcggc cttccgcaag ggtgtcccga
     5941 ttgtcttcag gccgaacggc ataggcgctc gtcgcgcaga gcccgctgac cgcgaggatg
     6001 acgaggtatt tatacatgct gatgcccatg gcgaatgttc gtctcctgcc gatctagtag
     6061 gccacgtcaa ttccggcaaa gaaggcttcc cgcagattcc tggctgccgc ttgcccgtct
     6121 tccgcgtggc gcagattgtg gtccaatcgc gcatacagtg cgatattcga ccgcacgtaa
     6181 tgcttcacct gggcgccgag gaagctttga gagcttcgtt ggctgaggtc tccccccgca
     6241 tccatagtgt cgtatcgcaa cgaaagcagc gtattgttcg ccacgtgcgt atccagcgcg
     6301 accgtgagcc cgctggcgtc cgtgtcaaaa ttgctgagag ccgtcgctgg tgcatgcgtg
     6361 attcgatcga gcgtatacat tccgtgcaga tcgaacattt tgtatcgcag gtgtgccgcg
     6421 acgccgtagc gactccagtt cacatccgtc gttcgtgtcc catcgaagac ggtcgcattg
     6481 ctattgccga gataggcaaa tcctgacagg ctggctgaaa aatttgctga gcgtgcgtaa
     6541 tcgaaccgga tggctccata gaagtctttg cctttattgg aatcgccgaa cccttcattg
     6601 gctccgttca gcacgccgag ttggtacatg aaccaatctc cgaagggccg cccccgtacc
     6661 tctgcgccgg tctctaccac tgcgttatag agcgaagggg catagggaga aatcgtgccc
     6721 cccgatctgt tccgcagtcc gtaaaatttg gctgccgttg ccagcggaaa caatcccgcg
     6781 cgttgaacgg catttgtcga ggggtgcgtg ctttccgcaa tgaagtccag ttgttggcgc
     6841 agtgttgaaa aggaaaaggc tgcggatggg tcgatctttc cgacgcggat attggcgacg
     6901 ttttcacctc cgatgttgtt gaaggtgagg aaggcccgct caattccggt gtgagatgct
     6961 tcgaggttgg attcgagttc ggcaaaaaac ccgatgtttt tcgctaaggt gccggcggta
     7021 aagaggctga acacttcggg gaaggccagg tcgctcttgt tttggaccgt gcctgcctct
     7081 gcaccggatg gattttgctg tttgaagtga tgggtgcgga gcacattacc gcgcaggcgg
     7141 acgccggtgt agttcgcaac atcgtccagg ctcacatccc ccagattttt ctttcccgtt
     7201 aggccgccgt ctgtggtgcc cgggagctga tagccatttt cgaggaagcg ttcgccaaat
     7261 gtattgagtc gtggaggggc tgtatggcac gcggtgcagt cgagtgcata cttgcgggca
     7321 aatgatggaa tggcgtctgc agtgggaacg gacacggcgt acattcctac aatgaagacc
     7381 agaaccgtca tcgtcagtcg gacaatgaca gcctttgcgg agcgcgtaat caaaacggca
     7441 ccctttcatg cccgagcgtg gtcgcgtgac ctcgcacccg tcctacacct tgactgtccg
     7501 atgtcggtgg attcgaagcg agactttagg cccaagggcg atcagcgcgg ttgctgaaga
     7561 aggggggcgt gatgcgatgt gctgaggagc tgctcggaat cggccaggag gagcaggtac
     7621 agcggggcgg aatgtcgaaa gatcagtgga tagttggcgg gctattcggg atattttgcc
     7681 tcgatgacgc tgcgcaaccc tccgcgagcg gcaaactcac ccgttacctt ggcccaagtc
     7741 ggtcggcagg cccgaaccac gtcttggagg atgcggttga cggcattttc gtagaaaata
     7801 ccgatgtttc tataggcgtg gacatacatt ttgagtgctt tgagctcaag gcactgttta
     7861 ttcggtttgt agtgaagagt aatcgttcca aagtccggga gattggtctt ggggcagatc
     7921 gccgtatatt caggaatgac aatcgtaatt tcatagccct tgtactggtt tggaaaggtt
     7981 tcaatgtctg gcaaaggggc cgagatcccg ctgcgtgcgt gccgctcgtt atagccgagc
     8041 ttggttgtgg agtttcctgt cttcaatttc ttcttgttct gttctctcac accatcaccc
     8101 cttaccggca atcgtcatac ctgtttcatc cattccaact gacccagctc attgagcttg
     8161 ttgtacagtg tgacccacgg acaggtcatg tccggataga cttcgcaaaa cccacctttg
     8221 cgcacccctc cgcaaggacc gtggctcatg aattttgggc actcggcctt aagggagccg
     8281 tctgggatcg gcgggcgctt atgcacgcct ccgacacgtt gatcaccctg ttcttcccct
     8341 ggtaccagaa ctcgtatcgc catagatgtt aggaggccta tcgtgttaag cgataatttt
     8401 tacgcgcttc tcagatgaac tgcaactgaa ttgaacctta tttcatgttt gttctttgct
     8461 caaaatcttt gagttcaagc ggatttaggg ccaagggcct agcaaggcgg catccactag
     8521 gccttctttc gttcagtgtg ggccttctgc aaagttgcaa tgcttttgtg gactctatag
     8581 acttgccgtt accgtggaat gacagggttc ttcctaattt ctagcgccga cttgattgac
     8641 agattttttt gcgctttgtt acactcggcg gcatcagaca gttaggtcgt aagtcgacat
     8701 tcctttccgt ttcaggaaca cagtccagta cttagttcga acaacgtcga tataatttgc
     8761 gcttggcttt ctccggccat tttggaatga cggaggaaag tcaccagagc ataaaaggga
     8821 gggaatcctt atgagtcttg attacgtcaa gttctcgagc ggctttgaaa agttcatgcc
     8881 gaaagaatat cgagacatgg ttgagcacgg cccgtttggg aaaaaggtct ctgtttcgca
     8941 gatggggtcc ttcaaggaag tgctggaaga gcaccccatg tgcgctggat gtgccatgac
     9001 cctctttatc cgccttgcca tgatcgcatt cccgaatccc gaagatacta tcactgttgg
     9061 aacagcgggc tgtggacgtt tggcaatctc gcaagctgcc atcccctttg tgtacggtaa
     9121 ctatggtgac caaaacggtg tggcgagtgg cttgtctcga gggttgcgcc ttcgttttgg
     9181 tgataagccg aaggacgtag tggtgatggc gggcgacggg ggtacggccg atatcggatt
     9241 ccagcaagtt cttcactcct ggttccggaa ggaacgcttt accacgatta tgttagacaa
     9301 cgaagtctat ggaaataccg gtgggcagga aagcggaatg acgaatcgtg gcgccgtgct
     9361 gaagatggct ccgttgggga aaaagtttga aaaaatggac atgttgcaga tggcgaaggt
     9421 cgccggctgc gcgtacgttg ccacggttgt cccgaacaat ccgcgccgcg ttgagagcgt
     9481 gattaagaag gctgtgctga tcgccagaga ggtcgggtcg acgtatatcc aagcctatac
     9541 ctcctgcaac atcgaatacg ccattccaac cgacaaggtc atggaagacg ccaagaacgt
     9601 tgaaaacgat cgctatcagt tctccgaata cattaccgac gaagcaaagc aatttctcac
     9661 ggagcgctat ggctacaagg agttcctccc gaagccggcg gctcccgctc ctggtctgcc
     9721 gaaggcctga gcaacgttta aggaggattc agcatggcga cacggttcaa tattcgtatg
     9781 gcaggtgtgg gtggccaggg cgttgtgacc ggctcgcaca ttctgagcac ggcggtcatt
     9841 catgccggcg gcgagagtac aatcgtaccg ttttatgggt cagaaaagcg catggcgccg
     9901 gttgagagct atgtcagggt ttcagatgag cctatttatg aaattggcga aatcactttc
     9961 cctcacatca ttatcatctt tcacccccag gttattacgc acggtaagtc gtacacgatg
    10021 ccattctatt tcggcttaaa agagaatggt atcgccttga tcaataatga cggcccgatg
    10081 aaattgcacc gcgatcaggc tcgcgaactc gaagagcggc atgcgaagct ctactacttc
    10141 cctgctacca agctttcgct tgaggttgcg gggatggacc ttgctacgaa tatggcgttg
    10201 atgggttgta tcggcgctat tactggtctc actacagtcg aggcactgga gcagtccgtt
    10261 aaggatcgat tcctcggaaa aggttttgtc gtatccggtg gaaccgcggc cttggatagc
    10321 gtcgtcgagc gaaagtttaa gaagaagcaa gaattgattg agaaaaatgt ggccgttatt
    10381 cgagcaggct ggaattatgc tgttgaccat ggatgggccg caccatccgt gaaacgttcg
    10441 gaagcgaccg aaagcgtcac ggcgtaatcc ggatcacgga caaggagttc tcatgtatct
    10501 cgtagccgat attagtgttg agatctgtgc cgcaaaaagt tgcaagctct gcacacagta
    10561 ttgcccggaa gccaatacca ttctttacag cgacgaaatg ggcaaggaca agggatttaa
    10621 gtatggctca gcttatgtgg ccgtagatcg gtgcaagggc tgcgcgcaat gtgtgtgggt
    10681 ctgcgacaat atggccaaaa ataatgccat taaaatgatt atgattgacc agttgccggt
    10741 ggcggcattg acggacaata ttacctatgg ggaaaaaagt acgacggcga cattggtcag
    10801 ccctgtagtt gggtaattgg acgaaagggg aacgggcgtg ggaacgacgc aagatacgag
    10861 agagcggatt atcgtgccag ggccggcagg gttccatcca ccgtcagcag cacagatggg
    10921 agtgtcgttg cctgatcctg ggcaaggact atattacggc ctcctcgaac ctaatgaaga
    10981 cacagtgatc gaagaaatgg ctcggaaaat gctgacgagc ccgaatgcaa cgatcttccc
    11041 aggacctctc gtcttgtggg cgtggaacaa ccatgcagtc gaaaaagcca aggccgttct
    11101 ggagatcgcg gcgcagatcc cggacgtcat gattattccc atgcctgatt atcgccccaa
    11161 gtatccaaag atcgatcctg aagaggtaat caatccgaat catcccaatc ttacaatttg
    11221 gggtaacaag atcgaggctt gtatctttat tggtgtgcat tgtcattacg ccaatctgac
    11281 cctcaaaatg atccgggcag ggacgaattg ctgcacaatg gctatctgcg cagaacaggg
    11341 ccatgaagac gccatgctga cgattcgaga ctccgacacc ctaaagctga aaaagactgc
    11401 gcagatcttc aaaaaggttc gtgaagaatt gggcattaaa ttgcctgaca atggggagaa
    11461 tgtccgcttc actggcactc agtcgaaagt ccacgatggc aagacgcata caaatcctat
    11521 gacttttatg ccttccgctg ccggtgcagg tagtgccgcc gcgtttggtc attctcctga
    11581 tcagatgaag cgagaaggct aggggtttag gctaaggcaa taagtcgaga ggtgtgatga
    11641 tgagcgaagc acaaaagacc ggtccccaga gcgagccagc cacgagcgtt tcgcctgcga
    11701 aagtagagac caagaaagac cctcatgcgg cggcaaagca acagaaggtt gtgacgccgg
    11761 aatatatgtt tctggaagct ccacggacca aagagtttat tactggcagc gaggcggcaa
    11821 aggaagccat ccgtcgctcc aacgttgacc tcgctatcgc ctatccgatt accccgcaaa
    11881 gtgaaaccat gcagctcgtc ggtgttctgt atggagaggg gtatgtgaag gaatattacc
    11941 gtggcgagga agaagttggc gtcatggccg ctatcgcggg tggttcgcga gcgggcgttc
    12001 gatgctacac cgccactgcg ggtcctggga ctttgagagg tcttgaaggt atcgcgtcat
    12061 ggcccggtca ccggctaccc gttgtggcga tgttcacctg tcgagtcgtg aacgcaccat
    12121 tggccattca gcctgacaat atcgaagtgt cgtatctgct caattgcggc atgattgtgt
    12181 tccatgctga aaatcagcaa gacatgtatg attttacgct ggcgggattt gtcatcagcg
    12241 aaaaaaatga tgtgactctt cctgtgggcg tgtgttgcga cgggttcttt gtgacgcatg
    12301 cgagaggcta tgtgcgcatg caagatcgtg gtatgaagct tccgccgcgt gagccttggc
    12361 gtggagctgt gccagtgttg gatgccgaga atcctccggc ccggctttct cgcgatgccc
    12421 ccgttcagaa gtcaaatttt atggcttaca acattcacgc tgtctggcaa caggaagtgt
    12481 gggctgcggt cgagcgctct cgaaagtaca ttaatcagta catgggcggg ctgctgacgg
    12541 ctgaaaatgt cgaggatgct gaggccatca tcattgcttc aggtagtgca gcggctcaat
    12601 ctcgcgaagc cgtccgcatt tgcgctgaaa agggtatgaa gattggcctg atcaaggtta
    12661 gatcccttcg tcccttcccc actcaagagt tgcgtaagct ctgtggaaaa gccaagctta
    12721 ttgtcgtgcc ggaatttaac tacgtcggat ggttggcaaa ggaagtggcg acagccatct
    12781 atggcttctc aaatgcgaag attatcggtg gaccacgggt ttatggtggt caatccatgc
    12841 cggtagaatt gatcgttgac gaagttgaat cagggctaac tggaaagaag tcgacgaacg
    12901 tagcgatttc tcaggtgatg ggcgcctcgg ctgcggatca cgaagccatg gggcatttca
    12961 tgcgcagcat ctaaggatta acggcgtaat cctcgtcaga taggagggcc cgttcggttg
    13021 acccgaacgg gccctctgca ttttttggtt ccaagcgatt aaccctgaag atcttcttcc
    13081 acgagatcag ggctttctgg cataccgtga gccacgtatt tggcgtacga caaataaatg
    13141 gatgcgctgt cacgcatggc tttggtgcca ctggccattc gatgcagctt atcagatccg
    13201 ggcgcctcgg tcttgcggag ataatcgaca tggtcatgaa gaactagcgt gaagcgctcc
    13261 atggccattt ctacctctcg gtaggcttgc aaaatggggt cagtcatgtg aagtactcct
    13321 ctcctatcca agataccgca gccgtcttta gagggtcaac caagaggccg actccatgta
    13381 ccaactgtca tcggcaatgt taggcacaat cgcaacggtg gctagggtca ataactccct
    13441 acagaatcca gcactttcag catcgctagt cagtggcgtg aaaaatcttt ctgaaatgtt
    13501 tacgagagga accaaagctg gtgcgacagc ctatcttgaa gatggggcgc tacgtgccag
    13561 ctaccttcat tattatgtgc ctgttaacgt cggcaagatc cagatgcttt tggaggaatt
    13621 gcggccgcta cttccatccc gtacaaatga agaatttcga gtgttggatg tcgggggcgg
    13681 acctggcacc ggctcactag cccttctcga ctggtgcagt tcacagtctt tgccacccat
    13741 aagcagtctc cgcatgacgg tggttgaccg ttcgatcgag gccatgaaac tgtcgacccg
    13801 gctctgggag gaatacgcca ggcagatcaa cataccttct attcaaccat tgcagactgc
    13861 cgtgtgcgat cttgagcact cctttcctcc agttcttcga atgggagcgg agaaaaatgc
    13921 ctatcacctt atcattgttc agaatgtgct ttcggaactg tttgccgaaa aatccgattg
    13981 tattgctcga cggacaacac tgatcaatca cctggtagac tgcttggcaa aagacggaag
    14041 cctaatgctc attgagccgg catcgagggc tgaatcgcgt gccctgcatc aggtgaggga
    14101 taatgtgctg tctgaattgc aatgctcgat ctacagtccc tgcctccatg accaactctg
    14161 tcccgcgttg ctcaaacctg acgattggtg tcatgaagag cgcgagtggc aaactccttc
    14221 ctggattagt cagatcgatc gcgaagtggg atttattaag gacgctctca aattttctta
    14281 tgtcatcata cgtaaagatg ggaagtcctt ggtgcctcga aagcaagatt atcaccgcgt
    14341 cgtaagcgag ttgcgggaga tgaaggggga aaaacgggtg tggttgtgcg atcagacggg
    14401 taggtctgaa gttggtcgac aagataagga gagatcaacc actaacgcca cgttcgacaa
    14461 ttggcatcgt ggggcaatcg ttcgcgttga cgagatcgtc cgaaaagaac ggaaaggccg
    14521 gccagccatg gtcggtcgaa ttctttcctc cggagtggcg gaagtcattc ggccggcgtg
    14581 aagcgctaat cggcttcagg ctctgaggag ccgtgctgat gagacacgcg atcctcatca
    14641 aagagtgcag ccatttcctc cgcaatcatg tcccgatcgt tgggcaccga gacgagagga
    14701 atttctttgc gaggctttgg tgcgacatca gcgacaggga ggggcggttt cggcttttgt
    14761 gtgtcggtca taataagtca gtatacacga aatgccgggg gcgagggcta ttcaaaagct
    14821 tccagggaag aacgctcggg aaactgagtg tggcatgcgc tgcacgtcac gaaatagatt
    14881 gcctcacgga cagacatcgt gatgcggaag tcgagagata cgccacgatg ttgacaattt
    14941 ggacaagaaa tttccttgag ccggtaagga atatcaggtt gtgttcgcag ataaaattcc
    15001 atgtcggtat ataccggaaa gctgtagcgg catttgaggc acaccccacg acattctccg
    15061 tccgattgca gagtccgccg atcaatgccg aatgtatttg tcttgcagat cgcgcattgt
    15121 acgctggaca ggcgcttttc tacctgttca agctgaataa gagccatcgt tattacccca
    15181 tcagccaagt ggttgaatga gtatacgcac tgcgttacgg aatcttcaat cgttgggcat
    15241 ggaatcgcag gaatccttga ccccctcccg cggaattcta tactaacctc accatgcaca
    15301 tgatcaataa gcgtctgctc gtcggtaatg ctgatgatgc gaagaacccg ccgccgcagg
    15361 tcggagcgat actcatggtg gcagaagaac aaaatgtgac tgtccccgca aggatcatct
    15421 atgccaagat tcccctgaag gagtttggtg agccagcggt gtcagccctc tacgacgcag
    15481 tgcaatggat tgaaaagcac ctgcctgaca atcggttgat ggtctgttgc cgggctggta
    15541 tggggcgttc ggtgtccgtt gtcatcgcct acctttgttg cgtggagggg atgacgtatt
    15601 ccgatgcact taagcttgcc ctcacaaggc gtcctggtgg tatgccgcta cctcgcctcg
    15661 ataaggtgat cgaggacgtt ttccgccgac gtcgtgctgt agagcgacct tctctctcct
    15721 agattgattc ccccgacatt attagcagca tcctttcact acaggcctcc cccctttcgc
    15781 atctattgcg ctgccaattg gtttaaagga actgccgttg ggagttgcat ttctccttgt
    15841 cgccatggcg taatttactg ttttttgtgt cattacctac atacggaaac ctatacatgc
    15901 ctgaattgcc ggaagctgaa gtggctgtgc gccagctacg cgagagagtg gttggagccc
    15961 tggtgagtga ttgctgggta gggcgggaag acattgtgcg ggaaggcttg tcttccttgg
    16021 aatggtttcg cggagcaacg attacagggg ttgagcgaag agggaaaagc gtcatcctcg
    16081 cttttagccg cgcagaagaa gtgaaattta tgactgccga attggggatg accggcctac
    16141 tcctgtttcg gaatacgccg actaaacacc cgcaacatac gcactttgtc ctgaagcttg
    16201 aaggaggcgc ggaatctgag atccgatatt ggaatccacg tcgattcgga cgtctctcgt
    16261 tattagacca agctggactt gatcgatata catcccgtcg ttttgggccc gatcctttag
    16321 tgataagtca aaagcagttt gtgcagttgc ttttatccac gcgtagtagg ctgaaacccc
    16381 tgttgatgca tcaacaggct atcgcaggaa ttgggaatat ttacgccaat gaaatgctgt
    16441 ttcgtgcccg acttcatcca gatcaaattg ctaatcaagt gaaccaaaag gcagcgatac
    16501 ggctgcatga aatcatgggg gacgtcttgc gagaggcaat tgatctgggt ggttccagtg
    16561 tgcgcgatta ttttgcgccg gatggaacag aggggcagta taagcgtcga caccttgtct
    16621 atgccaaatc aggcgagcct tgtccgaacg attgtggagc ggtgatcagg cgatccatcg
    16681 gcgagcgaag ttccttttat tgtcctcggt gccaacgcat tcgtcgcccg caagttggtt
    16741 agcatcggaa ggtccgtctc ggttctttca gataactaag gagacaattt aagcttatca
    16801 atcatttatg gtaatgaatc ttcagtgccc attatggtag gacttctgag ctcgaagcgt
    16861 aggccctttg gaggattgag ctaatccgca gaattcggta ctctgtcgct ccgtttctct
    16921 gccctatcac acaccaagga gtcgtcaatc atggcgaaag tgctggaagg tcccgggatg
    16981 gggctgatga agaagtgggg aatcaacgtt cctaattatg tggtcgtcac ttcagttgat
    17041 gaactgacca aattggggca agccaatgaa tggttgaaga agtcgaagtt ggtcgccaaa
    17101 gcgcatgaag cgctcggctc gcgttttaaa ctgggattgg tcaaggtcga tttagatttc
    17161 aaggccgcgg aagctgcgac caaggaaatg attggtcgcc aagtcggcag catcactgtg
    17221 acccaggtca ttgtctctga gatgatcccg cacaaggaag aatactattg tgctgtgaaa
    17281 tcgacacgcg aaggcagtga gatcctcgta gccaattgtg gcggaattga ggttgaatct
    17341 aactgggaac gtgtcaaacg tttgtgcctc gacatcgggc aatctccctc tcccgaatcc
    17401 ctcgaaaagc tcagcaaaga agccggattc tccggcgcgc ttgccaagaa aatggcggag
    17461 tttgctggga aaatgtttgc ctgcttcgat agcgaagacg cgcaatatct tgaagtgaat
    17521 ccggtagttc tccgagccgg cgatgatgaa ctggtggccc tggatgcagt cacgcttctc
    17581 gacggtgatg ccaaattcag gcaccctgat tggaactttg cctttgccgc ggaattcggg
    17641 cgagcatatt ccaaacaaga gcttgaggtg atggccgtcg atagcaagat caaagggtcc
    17701 gtcaaattca tcgaaatccc cgggggcgat accgccatgt tgcctgccgg cgggggcgcc
    17761 agtgtctatt actctgatgc tgtcgtcgct cgaggcggca agttggcaaa ctatgcggaa
    17821 tactcgggag atccacccga ttgggccgtt gaagtgctca ccgagaaagt ctgctctctt
    17881 cctggtatcc gaaacatcat cgtaggtggt gccatcgcaa atttcactga tgtaaaaaag
    17941 acattcggcg gcatcatcaa cggattcagg aaagcaaagt cggaaggcaa gctcaaaaat
    18001 gtgaagattt gggtgcgtcg tggcgggccg cgtgaaaaag aaggccttga tgccatgcgc
    18061 gcgctgaaag acgagggctt tgatatcaat gtcttcgacc gcaacacacc gcttaccgac
    18121 atcgtcgaca aggcacttca agcgaagtag cctgccgtct atgaccaact gaatataagg
    18181 ggagatcccg agatgagtat tctggcaaac aaagacaccc gcgtggtgat ccagggtggt
    18241 caagccggtg tcaacgccgc ccgccgcatg gccgagttct gttatctcat caagcgtccg
    18301 ctgaacgtcg aggcattcgt gtatccgcca gacgccggta agaccaatga gataccttac
    18361 ggcagcggcc tcatcgcgat tcctgtctac aagtctatcg ccgaagcgac gaagaatcac
    18421 ccgaccctca ataccagtct ggtctacatt ggcgcagacc gtgcgatgaa gggcggaatg
    18481 gaagcgcttg atgattcgca tatcaaggtg gtctcaatga tcactgaggg tgtgccggag
    18541 aaggacgcga agattctcgg cgcccatgcg cgcaagctgg gtaaggtttt caatggtcca
    18601 tcctctatcg gtattatttc agccggcgcc tgtcgtcttg gtgtgattgg tggtgcattt
    18661 gacaacctcg tcctttcgaa gctgtatcga gaaggatcgt tcggcgtcat taccaagtct
    18721 ggtggtctct cgaatgaaat catttggatc tgttcgcagt tcgccgacgg cattacgacg
    18781 gcgatcggga tcggtggcga tgcctaccct ggtacggatt acgtcagcta ccttgagatg
    18841 tttgaaaacg atccgcaaac gaaagccgtt gtgattgtgg gcgaaatggg cggcgacctc
    18901 gaggagcgcg cagctgaatg gtatggggcc aagaagcggc gagtgaagtt gatcgccgtg
    18961 gtatctggat tctgccagga gagtttgccc aaaggaatga aattcggcca tgccggtgcc
    19021 aaggaaggca tgaaaggcga agggtccgct cggtcgaagt ccgatgccct caagaaagcg
    19081 ggagcacttg tgccccctac ctttggcgcg cttggtcccg caattaagga aacgtatgag
    19141 gacctgctga agtccggcca agttaaggcg ccagtcgagc cggcggttct gcctcgctta
    19201 ccaaagaccg ttgaagaagc aatgaaggcc gatgaagtga tggttgcgcc actcattcgc
    19261 acgacaatca gcgatgaccg tggcgatgaa ccgtgctacg acggctatcc tgcctccgaa
    19321 cttatcaaca aaggctatga aatccctcac gtcatcggac tcttgtggga caaacgcttg
    19381 atctcgaagc aggaagccga aatcattaag cggattatga tgctctcggc ggatcatggt
    19441 ccctgcgtaa gcggcgccta cgcgacgatt cttgccgcct gtgccggcat cggcctttcc
    19501 cagtcagtgg cagccggcat gattatgatt ggtccccgtt ttggcggagc cgtgaccgat
    19561 gccggtcgct tctttaagta cgcagtcgat aacaagatgt cggtcgatga attcttagcc
    19621 tatatgaaga agaatcatgg tccggttcct ggtatcggcc atcgtgtcaa gagcctgcgt
    19681 aatccggaca agcgggtcaa ggaactggtt ggttatgtta agagcctgaa gatcaagacg
    19741 ccttgtttgg attttgcact tgaggtggag aagatcactt cggtgaaaaa ggataatctc
    19801 attctgaacg tcgacgggac catggcggct gtactcgtcg atatcgggtt cccggttgat
    19861 agtttgaacg gattcttcat cctttctcgc acgattggct tgattggcca ctgggtggat
    19921 cagaagcggc aagatagccg cttgattcgg ttgttcgatt atctggtgaa ttacgcggcc
    19981 cccaaacgcc gcgaagtgcc gccgctgaag tagcgcaaaa cgtgacgtgt tgtttgcgaa
    20041 aaggatcctg ctagacaacc tccttcgcag cgaacgagtc gcgcaaccta tagatcgtac
    20101 taacagacgg agaacctact atgtccatgg atctcgccaa agcgctctat gccaagatgc
    20161 cggaggtttt tgcaaaggcc agaaagaagt tcggtcgtgg tttgacgctt gccgaaaaga
    20221 ttcttgtctc acatgcggac aactttgata gccagaattg ggaacggggc aaggccatgc
    20281 tcgctctccg gccagaccgc gtggctatgc aggacgccac ggcccagatg gccgtgctgc
    20341 aattcatgca ggccaacaag aaaaaggcgg cggtgcccag tacgatccat tgtgaccatt
    20401 tgatccgtgc cgaaatggga tccgagaagg atctccttcg cgcgctcgat gagaacaagg
    20461 aggtttataa cttcctcgcc tcagctgcaa aaaagtatgg cattgggttt tggaagccag
    20521 gcgccggaat catccaccag gtcgttttgg agaactacgc gtttcccggc tgcttgatca
    20581 ttggtaccga ctcacacacc ccgaacggcg gcggcttggg aggactggcg atcggtgttg
    20641 gtggggcgga tgccggcgaa gtcatggctg ggcttccctg ggaagtgctg catcccaaac
    20701 tgatcggcgt gcgattgacc ggaaagctga atggctgggc ttcccccaaa gacgtgattc
    20761 tctatctgtg tggcctcctt actgtaaaag gcggcaccaa caagattgtc gagtactttg
    20821 gtccgggtgc tgaaaccatc agcgccacgg gtaaaggcac catctgcaat atgggggcag
    20881 aattaggcgc gacgacctcc gtcttcccgt tcgacgagaa gatggtggcg tacatgaaga
    20941 ttaccgaccg cgccgatctg gccaccttca cgcagtcaca caaagatctg ctggtggcgg
    21001 atcctgaagt cgctcagtca cctgaaaaat actacgatca aattgttgag atcgatctgt
    21061 caaagctgga gccgcacgtt gtcggtcccc acaccccgga tctggcgcgg ccaatttcaa
    21121 agttgaaagc tgaggcgcag gaaaagggat atccggttga actgaaggcg gcgttgatcg
    21181 gcagttgtac gaattcctca tatgaggaca tcagccgttc ggcccacgtg gctcaacagg
    21241 cattgaaggc aggcttgaag gcgaaggctt catttctgat ctctcctggc tccgaacgca
    21301 tttatcacac gatgaagcgg gacgggttta tggatacgtt cgaaaagctc ggcggaaccg
    21361 tgttgtctaa ttcctgcggc ccctgcatcg ggcagtggaa gcggtcagat ggagtcaagg
    21421 gtcgtgcgga ctccattgtc agttccttca atcgtaactt ccccggacgt aacgacggca
    21481 tcagcgaaac gctgtctttc ctcgccagtc ccgaagttgt gactgcctac gccatcactg
    21541 gtgatttggg ttttgaccca gtcaaccaat cggttaaggg ggcagatggg aaagagttta
    21601 agcttcagcc gcccgttgga gaggaattac cagccaaggg atttgcgaag ggtgaagaag
    21661 gattcgtagc gcccgcagag gacggctcga acctgaccgt ggatattccg ccgaccagcg
    21721 aacggttgca attgttgcag cctttcccga agtgggatgg taaagacttc gagaagttgc
    21781 cgctcctgat caagacgaaa ggtaagacta cgacagatca tatttcgccg gcagggccat
    21841 ggctcaagtt ccgcggtcac ttagacaaga tcagcgacaa tatgttttta ggcgccaata
    21901 atagcttttc gtcggagcct ggtaagggaa ccaacgtgct gactggtgaa tccaatctta
    21961 cgatggccca aattgcacgc gcctacaagg ccaaaggcat cggatcgatt gtcgtgggag
    22021 acgagaatta cggggaaggc agtagccgcg aacatgccgc aatgtctcca cgcttcttga
    22081 atgtccgggc tgttatcacc aaaagctttg cccgcattca tgaaaccaat ttaaagaagc
    22141 aaggcatcct cccgctcaca tttgccgatc ctaaggacta tgaaaagatc gagcaaaacg
    22201 atcgtatcag cgtcgtggac ctggccaatt tggcgcccgg caagcccgtg acagtggtca
    22261 ttcataagac tggtggtgat ctgaggattc agacgaatca cagcatgacc gctcagcaaa
    22321 tcacctggtt caaggccgga tcagcgttga atgctttgaa ctaaacaata cagcaaaggg
    22381 agaagcgcaa tggcaaaagc agataaaatt atctatacga agaccgacga agcgccgatg
    22441 ctggcgactt attcgttctt gccgatcatc aatgccttca cgaaagcggc tggcgtgacc
    22501 gttgagttgc gcgatatttc tcttgcgggc cgcgtgattg cagtattccc ggaatacctt
    22561 actgccgaac agaaacagca cgacgccctc gccgagttag gggagatggc gaagaccccc
    22621 gaagccaaca ttatcaagct gccgaacatc agcgcctcta ttccgcagct cgtggccacc
    22681 atcaaggaac tgcagagcca ggggtataag ctcccagact atcccgagaa tcctaaggac
    22741 gataaagaga aagacatcaa gtcccgctac gacaaagtca aaggcagcgc ggtcaatccg
    22801 gtactgcgcg aaggcaactc cgatcgccgc gccccattgt ccgtcaaagc ctatgcccgg
    22861 aagcacccgc ataagatgtc tccctggagt cccgattcca agacccatgt ttctcatatg
    22921 aaaggcggag acttccgttc gaatgagaag tcaatgacga ttccggccgc gacgacggcg
    22981 aagattgagt tcgtcggtgc cgatggcaag accaccgttt tgaaggagaa ggtcgctctg
    23041 caggctggtg aagtgctcga cgcgacgttc atgagcgtca aggcgttgcg cacgttcttg
    23101 gaggagcaga tcgaagatgc caagaagcag ggtgtccttt ggtcgcttca tatgaaggcg
    23161 accatgatga aggtctccga tccgaagatc ttcggtcatg gcgtgaccgt gtactacaag
    23221 gatgtatttg agaagcatgc cgagacgttc aagaagctcg gcgtcgatcc ggacaacggt
    23281 ctcggtgacg tatatgcgaa gatcaagtcc ctgccggacg accagcgcaa ggcgatcgaa
    23341 gccgatatcc aggcggtcta tcagaagcgc ccgccgatgg cgatggtgaa cagcgacaag
    23401 gggatcacca atcttcatgt gccgagcgac atcatcatcg acgcatccat gcctcccgtc
    23461 atccgcgaca gcgggaagat gtgggggcca gacggcaagc tggccgatgt gaagtgcgtg
    23521 atcccagacg ccagttacgc gccgatctac cacgaggtcg tcgagttctg taagcaaaaa
    23581 ggccaattcg acccccgcac gatgggcacg atcccgaatg tcgggttgat ggcccaggcg
    23641 gcagaagagt acggttcaca cgacaagacc ttcaaggcgc cagccaacgg caccatccgc
    23701 atcgtggatg cgaacggcac ggtcttgcac cagcatcagg tggaagccgg cgatatttgg
    23761 cgtgcctgcc aggttaaaga tgccccgatt caggattggg tcaagttggc cgttacgcgc
    23821 gctcgcgcaa caggcgcccc tgccgtgttc tggctggaca agacccgcgc gcatgacgcg
    23881 gaattgatca agaaagtgaa cgcctatctt ccgaagcacg acacgaacgg tctggagatt
    23941 aagattatgt cgccagccga agcctgccgc ttctccatcg agcggatgaa ggaaggcaag
    24001 gacacgattt ccgtcaccgg taacgtgctc cgcgattatc tcaccgatct gttcccgatt
    24061 ctcgaaatcg gcactagcgc caagatgctc tcgatcgtcc cgttgctcaa cggcggcggc
    24121 ttgttcgaaa ccggcgcggg gggatcggct ccgaagcatg tgcagcaatt ccaggaagaa
    24181 ggctatttgc gttgggattc tctcggtgaa ttcctcgcac tggccgcatc gcttgagcat
    24241 ctggcgaagg tgggcaacaa tccggtggcg aagattttgg cggatacgct ggatcaagcc
    24301 aacgcgaagt tccttgagag caataagtca cccgcgcgta aggtcggcga gatcgacaac
    24361 cgcggcagtc acttctacct cgcgctctac tgggcgcagg cgttggcggc ccaaaccgcg
    24421 gacaagaaga tcgctgagcg atttgcgaaa attgcaaagg atttgagcga caacgagaag
    24481 aagatcgacg gcgaactgct tgccgcgcag ggtaagcccc aggacgtcgg cgggtattac
    24541 cacccgaacg atgcgaaagc cgctaaagcc ctgcggccaa gcgcaacgct gaacgcaatc
    24601 attgacgcga ttgcttaagc gatcatgatt tgatccggtc aggcgcaaga aggaccaacg
    24661 tccttcttgc gccgcacctt tttacgattg acgagaaagc catggcacaa gaagacacca
    24721 atgtgatcga tcagccggaa gtattgcgcc acaaaatggt gaccattgag atttccggta
    24781 aaaagtacga agtgccggaa ggtattacgg tgatcaaggc cttgtggcat actggccagg
    24841 aggtcgtgag aggcgcaggg tgtctcggtg gtttctgtgg cgcctgtgca acatattacc
    24901 gcacgaagga cgatccgaag gttcgaacct gtttggcttg tcagatggcg gttgaagacg
    24961 gcatgtcctt taccatcatg cccccgtttc cagcccgcaa agcgctttac gacattcagt
    25021 cgcttaaaga tcccaagcaa gacctcttca atttgtaccc cgaggcgccg ctctgcagaa
    25081 actgtaacgc ttgcacggaa gcctgccccc agaaaattga tgtgcgtgaa ggcgtctgga
    25141 aagcagtatt tggcgacttc aaaagcgtgt cagaaatgtt tatggattgt gtcatgtgcg
    25201 gcatgtgcac gcccgtctgt attgccgaca tcgcgccaaa cctggtggcc ttatatgtca
    25261 gccgggccca aggagcgcat tttaccgaga agccccaggg ccttgatctc cgcattaaag
    25321 aaattcaaga cggtcgattc aacgacgagt ggaacaaagt gctcgcgatg aacgaacagg
    25381 agttggccga tcactgtgcg accgtaaagt aatgcattgt gccgttagcc ggttctgagc
    25441 gcatggctcg ggtactgtgc tccgcgctga aagtgaaacc atggatattc atgcactcca
    25501 gcagattgtt caccaaaccc gagatgctcg tcgcaagcag accattccaa agttttcccc
    25561 tgccgaccgc gaccagttaa ttcataagta tcaccccgat tatcgggcga gtgcctatcg
    25621 gccgattcga tttgggccca atgccgacga caaaaccgtt gtcgagctgg cggcgctgct
    25681 tgaaggcgat agctctatcc ccgagcatct cgacttgaca cctcaatata cggccgacgt
    25741 cctagttatt ggcggcgggg gagcagggtg tgcggcggca cttcacgctc acacatccgg
    25801 tgcgaaggtg atcctggcaa caaaacttcg tcttggcgat tccaacagcg tgatggctca
    25861 aggaggcatg caaatctccg ttgcccccga agactctccg gtcacacatt ttcttgatac
    25921 cctcaagggc ggacacatgc agaacgacca tgagctgctc aaggtcatgg ttgaagaggg
    25981 gccatccatt gccaaatggc tgattgaact cggggtactc tttgaccggc aagccgacgg
    26041 caatttgcat gtcaaaaaag gtggaggaag ctcgaagccg cgtcttctca cctgctcgga
    26101 ctacaccggc ctcgaaatca tgcgggtcct gaaggacgag gtgctgaacc aaaagattca
    26161 gctacttgag ttctgtgcag ctgtcgaatt attgagcgat gataacggtc agtgcaccgg
    26221 agccatattt aaggatctcg acaaccaccg tcatgtcatt gtcgccgcca agtcggttat
    26281 tctggccacc ggtggaatcg gtcgcttaca tatccaggga ttcccaacca gcaatcacta
    26341 cggtgctacc ggtgacgggt tatgcctctc gtatcggatg ggagccaagc ttgaccatat
    26401 cgataccttt cagtatcacc catcaggcgc ggtgtaccct gagcagttga tcggggctct
    26461 ggtcacggaa ggtattcggt cagagggtgg ccatcttgtc aacgccaagg gagagcggtt
    26521 tgtcaatgag ttggacactc gcgatgtcgt ctcctcctcg atcattcggg agtgtgagga
    26581 aggtcgtggt attcgcacga tgtcaggtcg gatcggagtg tggctggata cgccgttgct
    26641 tgatgcggag catggccccg ggacggtaga gaagcatttc cctgcgatga tgatgcagtt
    26701 cgaacggttc ggaatcgaca tcagtaaaga tcccgtgctt atctacccca ccttgcacta
    26761 tcagaacggc ggagtgaaga tcgatgcgaa ctcggaaacc aacgtgaaga acttgtttgt
    26821 cgcgggagaa gcatccggag gactgcatgg gcgaaatcga ttgatgggca attccttgct
    26881 tgatctgatg gtcttcggta aacgttcggg tttaaccgcc gcatcacgtg ccggatccat
    26941 gtctcaagga aaactgacgc tgaaccattt gaagcggttt cgagctgagg cgaagaagca
    27001 tggaaattcc agtggagtta tttctcccat gattcttccc gcctataccc gcaaagaacc
    27061 agagcgtacc gctaccaaat gatcgtacgt ttttacagcc gaacatcact gggtttctgc
    27121 gcacactatg tcgtttttca taagccacta gctcgtatga ggattcaatg aacgttcatg
    27181 agtttcaggc caagcagtta tttgcacagt ttggggtgcc agtgccgcgt ggaaaagaaa
    27241 ttacctcgcc ggaaggagcg actgcgtggg ccaatgaact aaatacccct gtgtttgtcg
    27301 taaaggctca aattcatgcg ggcggacgag ggaaagccgg tggtgtcaag atcacgaaag
    27361 ataggggtgc catcgcaggg ctcgccaaag aattgatcgg gaagactctg atcacgcatc
    27421 aaacgggacc gaaaggcagg acagtccatc ggttgcttct tgaagaaggt gcgaatatcg
    27481 ccaaggagct ctatctcagt attttggtcg atcgcgatac cggctggccc acctttattg
    27541 ccagtactga aggtggcatg gagatcgaag aggtcgccga gaaaacccca gaaaagatta
    27601 ttaaggaagc gattgatccg gctgtcggat ttcaagggca caatggtcgc aatgtggcct
    27661 tcgccttggg gcttcagagc atggagccgg ccgtcatcaa cccgttcgtg cagatgctcg
    27721 gtaatcttta tcgcctgttc atggagaaac atgccgcgct cgtcgaaatc aaccccttaa
    27781 tcatcaccaa ggaaaagaca ctggtggcgt tggacgggaa ggtgtcgttt gacgacaacg
    27841 gaatttttaa acatgaagat gtgcagaaaa tgcgggatct caatgaagag gacccgttgg
    27901 agatcgaggc cacggcgaat aatctcaatt atgtaaagct tgatggcaat atcggctgca
    27961 tggtgaatgg cgcaggtctt gcgatggcga cgatggatgt gatcaagctg gccggcagtg
    28021 aacctgccaa ttttctagat gtgggcggcg gggccacgaa agacacggta gcggcaggct
    28081 tccgaattct gctgaaggac ccgaacgtca aaggtatctt tatcaacata ttcggcggca
    28141 ttgtccggtg cgaacgtatc gcgcatggcg tgatcgaagc ggcgaaagaa gtgaagatta
    28201 cggtgccctt agtggttcgc ctccaaggta ccaatgcaga tgagggacgg aaactgttgg
    28261 cagagtctgg actcaagtta gaggttgcca acgatctgtg ggaagcagcg cagaaaatcg
    28321 tcaaactgac cggcaaggcg gcgtaaagga gaagaccgtg agcattctcg tcaacaagaa
    28381 tacacgagtg gtggtacagg gaatcacggg gaaggaagga tctttccacg catcgcagtg
    28441 caaggcttac gggacgaaga tggtcgctgg agtcaccccc ggcaaggcag gccaggaagt
    28501 tgaagggatc cctgtcttta atacggtagc cgatgcggtg aaaaagaccg actgcgatac
    28561 ctctctcatt tttgtgcccc cgcccttctg tgcagacgct atcctggaag ctgccgatgc
    28621 aggcgtgaaa ttgatcatct gcattaccga aggaattccc gtcaatgata tggtgaaggt
    28681 gaagcgggca ctacatggac gtgatgtgcg ccttatcggg ccaaactgtc ctggagtcat
    28741 tacggtcgac gaagcgaaaa tcggtatcat gcctggattc atccataagc gcggggtggt
    28801 tggagtcgtc tcgcgcagtg ggaccttaac gtatgaggcg gtgcaccaac tctctacgtt
    28861 aggacttggt gagacgacgt gtgtgggtat cggtggagat ccagttaatg gcaccggctt
    28921 cgtggatgtg ttgccactct ttgagaagga ccccgaaacg caggctgtgg ttatgatcgg
    28981 ggaaattggt ggcgacgcgg aagaaaaggc ggcagaatac attaaacagc acgtgaaaaa
    29041 gccggttatc agctttatcg ccggtatcac ggcgcctcct ggtcgccgta tgggacatgc
    29101 tggtgcgatt atttctggtg gtaaaggtac ggcaacagag aaaatgaagg ctcttgaatc
    29161 cgctggagtt cgagtcgtca agaatcctgc cgaaattggg catgcggtga tgaaggcact
    29221 tggccggtaa ctcatctcca gtatgtatcc actgaagggc gtcggaccgg tccgacgccc
    29281 tctttgtttt tggcgacgtg tgcagctgta tgggtgaatc actggacgcc ttcgaatttt
    29341 gacgcccatg tcagaatctt cccttttccc tccatgccag acgtggtact gtatggcgcc
    29401 cagcgtgagg cgattaaccc tcgaacgttg gcccgcctga tcggatctgg tttctttccg
    29461 gagccgcaac cgtccggtaa gggtccgaac tgtccatgaa aggacgccac tatgctcgtg
    29521 tttctcatac atgtgcgtga tcctcagttc tacgcgcttc cggccaagac gcgagcgaag
    29581 aatggccgta ttcgggtcat ggggttcccg ccgatcggca ttatgtctct ctcggccgtc
    29641 cttaaacggg caggtcacga atgtgtcatg ttcgatcagg cgcaccctga gacaccgaac
    29701 gaatttatca ttgaagaaat aaagcgacga aagccggcgc tcgtcgggat gagctttctc
    29761 agtacgacga gttatcctta cgcgaagatt ctcgcccgcc agattcgcgt ggcggatggc
    29821 tcggtgaagc tggccttcgg tggcgtattt gccagcctga acgcgggcct ggtcaagttg
    29881 caatgtccgg aggtcgactt tgtgtgccgc ggtgacgggg aacagttgct cctggagttg
    29941 ctcgaccata tcgacgaccc caaggatgtg gctggcgtga catgggcgaa agacggaaaa
    30001 gtcatcaata acccgaatcg ggccatggag cgacatctgg accagtggcc tttcccggat
    30061 cgggaaagtt tagagctaga cttcgtcgaa tccatgccgt tggacgtccc agcagtcctg
    30121 tccatggagc gctttacgac catgcagacg tcccgaggtt gcccatggcc ctgcgtattc
    30181 tgtgatattc cgatcttcaa cgaaggtaaa tggcgagcgc gcagcgccca gcatgtcgtc
    30241 gacgagctga agtttttaga agcaaacggg tacggctcgg tttatttcgt ggacgatcac
    30301 ttcttactgc agccgaagcg tattgatgca atttgcaatg gcatcattga tgccaagctt
    30361 tctattcaat ggggaattga aggccgcgtg gattcggtcg cacagcatct gtttcccgcc
    30421 atggcgaaag cccattgccg aacggtgatg tttggcatcg aaagcgggag ccagaaaatt
    30481 ctcgatcgtt tgcagaagga acagacgttg gccgaggtag agactgcagt gagaaatgct
    30541 aaaaaagccg gcattgaaat tgtccacggc tttttcaccg taggaaatcc tgatgagact
    30601 gtggaggaca tggaaaaaac attcgacttc gcgtcgaagc ttccattgga tacgtttgga
    30661 ttcaatcgac tgtgtgtcta tcgggggacg cccttgtggc aggagtacat caagcgtgga
    30721 ttggtgagtg atgctaagga ttggtacaaa tattttaagt gttcggaaat cgatcctaca
    30781 tgcttgccag gcgcgaccat caattcggtc cggcaggagg ggcttaagaa gctgttcatc
    30841 tacaagctta cccgctatcc aattcagacg ttcaagttgc tccgccgatt cttgcgctat
    30901 atgccattcc gcgatgttgc ctatctcatc atcaagccat ttctggggca gaagaagggg
    30961 gcaactaagg cagaggtgct ctcacgcgcc gtggaacatg cggagatgaa agatgcggct
    31021 gcgcagctga cgcagttgag tgatgaatta ttgcatagcg tgctggaaga atcgaaagcg
    31081 gagcgccagc gcattcagca ggaagccgag agctctcggg agttgcccat gatttcagcc
    31141 cggtagtgtc ccgacattca tgatcttcag cagttgatct gcccacatcc agggtgagcc
    31201 ttcgtcctca tgtttgcagt aggcgtagca atccttccca ttccgtctcg tctgtagaaa
    31261 gtattcaccc caccgcgtta agtctttgtt cgaatattgg ctgcggcgca gacgggcgta
    31321 aaggaagtca gccgtgtctc ggtattccgc gggtcgatca tccgtttcaa cagccgccca
    31381 cgcgacgcga aaccggcgca agaggttttc cacagtggca tgattccagc tgtcatgccg
    31441 gaattccatg acccagcgat acgtgggggg caggccttgc aagaattcag ctaggcggtc
    31501 atcgtttcgt ttgaaattgg gaggcagttg aagaaggatg gggcctagct tctgtttctc
    31561 ggctaaggga gcgagatgct ccaacatggt gtgaagaaag gacgccgcat cgggcctcag
    31621 tcgcttcacg tgggtgatgg tacgatgtgc ctttactgaa aagacaaaat gagccggagt
    31681 ctgctcaatc cattttgtca ctgccgacag ggtaggcagc cgaaaccata ctccatccag
    31741 ttcgactgtg cggtatcgcg ttgaataata ctccagaaat tgtgcttgct tgagcgttgg
    31801 agggtaaaat cgagtcggcc cctgccatgg cttataagaa taccctgaca agccgatgcg
    31861 gacagtcgcc atagagacct gaccttgctt cggcgagaca cgttcttcac cgaggtaaac
    31921 tgctacctgc ctagactgat gtgtccggca acacgcttga gaaaagcctg caattcagtt
    31981 tggagcatcc ccagcgtgaa tgcaatcgcg aggctatcgg ccatgcacct atcgattggc
    32041 atcgaagcac gaaaaagcca tcatcaatgc gagtgcattc tcaggtcttg agtggtttag
    32101 tgaggagacg ctgtttgcgg ctcgcatgcc attcagaaaa ggcaagcggc cggtgcggga
    32161 gccgcaaccg accgcttgcg agcacgggaa tacgggtttt ggattaggtt cccatttccc
    32221 acgaagccag atacttcacc tgctctttgg tgagtttgtc gatcgacaca cccatgccgg
    32281 cgagtttgag gcgggagatc tctttgtcga tatccgccgg caccgggtac accttcttct
    32341 ccagtttctt gtagtttttc acgataaact ccgcgcccag cgcctggttg gcgaagctca
    32401 tgtccatgac gctggagggg tgaccttcag cggtcgccaa attcacgagg cgtccttctc
    32461 caaggaggct gacgcgacgg ccatttttca aggtgtattg atccactccg gagcgcacgg
    32521 caagtttctt cttgctgagt ttctcgagtg cggggatgtc caattcaacg ttgaaatgtc
    32581 cggagttgca tacgatcgct ccgtctttca ttgctgcgaa atgttcaccg cgaatgacct
    32641 tgagattacc ggtgacggtc acaaagaagt ctccgagcgg cgcagcttcg tccatgggca
    32701 taacgcgaaa tccgtccatg accgcttcga gccccttgag tgggtcgatt tccgtcacga
    32761 tcacattggc acccatgccc cgcgcgcgcg tggcgatgcc gcgtccgcac caaccgtagc
    32821 cggccacaac cagcgtcgaa ccacagacca accgattggt ggcgcgaacg atgccgtcca
    32881 tggtgctctg gcccgtgccg taccggttat cgaacatgtg tttcgtgtcc gcgtcgttca
    32941 cggaaatgac ggggaacttc aatactttct tctcggccat gctgcgaagc cgaatgacgc
    33001 cggtcgtggt ttcttcggtt ccaccaatga catggcgaag cagttctttt cgttttgagt
    33061 gtaaatggga aacgacatcg gcgccgtcgt ccatcgaaag gtggggcttg tgagcgatgg
    33121 ccgattcaat gtgccgataa taggtcttgt tgtcctcccc cttgacggca aaagtcggga
    33181 tgccttcgtg tttcaccagg gcggctgcga cgtcatcctg cgtgctgagt gggttagagg
    33241 cgcagaggcg aacgtcggcc cctccggcct tgagcgtctt cataagattg gcggtctcag
    33301 tcgtgacatg gagacaggcg gttgcgcgaa tgcctctaaa cggctgctcc ttttcaaagc
    33361 gttttcgaat caatcgcaac accggcattg tggcttcggc ccattcgatc ttcagtttgc
    33421 cttgatccgc caggcccata tctttcacgt cgtaatccac tggttcctcc tcctgtgtcg
    33481 tgagtgatga ttgatcttcg attgcagaaa aaagggggcg ggcttacaac ccgcccccct
    33541 cggctggtat cacgcgtgac gggaccttag aggcccgcat ccttacgcaa agctttcgct
    33601 ttgtcggtct tttcccacgt aaactccggt tcgttgcgac caaagtgtcc ataggcggcc
    33661 gtcttcctga aaatgggccg acgcagtttc aagtggtcga taatcccgcg cggggtcagc
    33721 gggaaatgtt tccgcaccaa cttatcgagg ctttcgggag ccaccttctc cgtgtctttg
    33781 gtgtcgacca ataccgagac tggatctgcg acgccgatag cataggccag ctggacttcg
    33841 catttcgagg ccagcccggc ggcaacaatg ttcttggcaa tatagcgagc catatatgag
    33901 gctgagcggt cgaccttgga tgggtctttg cccgagaacg cacctccgcc gtggctgcca
    33961 tgtccgccgt aggtgtccac aatgatcttg cgtccggtca aaccggtgtc acccatagga
    34021 ccgccgacga cgaatcgacc ggtaggattg atatgatgtt tcacgctggt tgcgtcgtac
    34081 aggcctttcg gcatggaggg cttaatgacg tattccatca gatctcgctc gatctgttta
    34141 ttggtcacat ccgggctgtg ttgcgtggag acgacgatgg tatcaatacg gaagggcttg
    34201 ccatccttgt attcgactgt gacttgggat ttgccgtcgg ggcggaccca cttcaagact
    34261 tttttcttcc gtacttcagc cagacgtttc gtcaagcgat gggccaaaac gatcggcatg
    34321 ggcatgagtt cggtcgtttc gttggtcgca taaccaaaca tcagtccctg atcgcccgcc
    34381 ccgccggaat cgactcccat cgcaatatcg ctggattgtt gatggataga ggtcaggact
    34441 gagcacgtat tagagtcgaa tccccaggag gcgtctgtat atccgacatc cttgatcacg
    34501 tctcggatga tatcgggaat ctccacatag gctttcgtag agatttcgcc ggcgacgaag
    34561 gcgataccgg ttgtcaggat ggtttcacac gcgactctcg atgtcttgtc ttgggcgatg
    34621 atggcgtcta gaattccatc tgaaatctga tcggcgattt tgtccgggtg tccttcagtg
    34681 accgactcag acgtaaacag gtagttatgt ctcattgttc cccctggaag tgaagtgatt
    34741 cgaggatgcg gtggttagag ttaggcggca caacgaccga gcgtggaaga tgctgcacgt
    34801 gcacagataa ggcaaccccg caaaaacgct acggcaatag tagctgaccg ggtggttttt
    34861 gtctaccgtt ctgacggaat tgatagcgtc tttcgcgaga ctcgggtctt gcttgacagc
    34921 tcttcgagac gacttgtaag atagcttact tttcgcgtat ttggaacatt tttcatcaaa
    34981 cgctctgccc cggcactccc aacctcgtgg tatcggcctg catggatgca gggtcccaca
    35041 cgcagctcgt cattcgttat gatgatggga tctgtatgac ggccaggtcg aagcagcgag
    35101 gtgtgtggat tcatgaacga taaacccgac gcagcatcag agatgagcgc ctccggccct
    35161 tccacgtccg gcctcgggtg ggaagagttt tcgcgcgaag tcgtagactt tttcgcttcc
    35221 attaagctgg cgatgttcct gttcatcgtg ctggccatga cggcgaccat cggcacagtg
    35281 atccagcaag gtgagcgtcc ggaaacatac gtgcaggagt atggggaaga ggcctatcga
    35341 tggtttcttc gtctgggctt tacggacgtc tatcacacct ggtggtttac cggtctctta
    35401 ggccttctct gcgtcaactc gctgacctgt ttccataaac gatttcccag cgtctggcgc
    35461 tccatgcgtc aggataaagt cagtgtttcc ttggcattca ttcaagggat gaagcagcag
    35521 accaccctct cgctcaatca accgaaagaa acagttgcgg aatccctggt caggcttttt
    35581 gcggaaaaag gctacaaggt gctggctaag agtgatcccg gcgaggtcac cgtctatgcg
    35641 accaaaggaa tcatgggccg ggtcggcgcg catgtcgccc atctaagtgc gacagtgatt
    35701 gtccttgggg ggctgctcgg cagctattac ggcttttccg agttcggcgt gtgtctagag
    35761 gggcagacgt accatatacc cagaggaaac tttgacctgc gggtcgataa gttctggatc
    35821 gactatcacg agaatggctc cgtcaagtcc tacaacagca ccttgaccgt catcgatcaa
    35881 ggcgctccga ccatgacgaa gacgatcacg gtcaacgacc ccttggtcta taaggggatc
    35941 tggttttacc agtccagtta cggggatgcc tgggaccaaa tcgaggccgc gcggatcaat
    36001 attaaggata aggcgtcgga caaggttgtg gcgacggtgg atttggagtg gaataaggaa
    36061 aaagcggtcg agggtcttcc gttgaaaatg aagatgacgg acttcgtggc cgacttcgcg
    36121 ttcaattcga ctgaaaagaa ggtcttttcc aagaccgccg aacatagcaa tcctgctatt
    36181 cgtctggcgg ttgatgagcg cagcaccgtg caatcgaccc cctgggtgtt ttatcattac
    36241 cccgacctgt ttgagatcaa ggattccgcc tatcagtttg aatttatcgg gtatcaaccg
    36301 aagaaattca ccggtctgca gattgccagg aatccgggca tcatgatggt atggatcggg
    36361 tgtacgatgt tggtggtagg gatgacattg tcatccctca tctatcaccg gcggctgtgg
    36421 gcgaggattc ttcccggcga atcgggcgtg acggttcatt tgggcggaac cacgcacaaa
    36481 agccagatcg acttccaaaa agagttcaag aaattgacgg agaagattca agccaagact
    36541 tcttcttaaa gcccgtttca gcgccgttga cggatattcc attcaggacg acgctgcact
    36601 ctacgataga cggaggcccg tatgatgcga tcattgtttt tgttcgatat gacgttctgg
    36661 ctctatctcg cagcgctggg cctctatgtt gcctatctgt tcgcaaagcg tccggctatg
    36721 cagatggctc cggcggggca tcccattgag aacctggaag accgtgacgg ggcttgggcg
    36781 actcaattag gacaggtggc caccctggtc accgtgtttg ggtgggtgct caataccatg
    36841 gccctgatga cccgggcgtt cgagcgcatg gagcattccg ggacctttgc cccctggtcc
    36901 aatcaatttg aagccatggc ctacgtttcg tgggcgatca ttctcggcta cgtgctgctg
    36961 gagtttcggt accggatcaa ggctgtcgga gcgtttgtgg tggggatcgg gtttatcgcg
    37021 atgggcgcag cgtcgctgct gccctatcgc taccagacgg cagaaccgct ggtgccggcg
    37081 ttaaatagtt attggatcta catccatgta tccgtaaccc taaccagcta tgcggcattc
    37141 gccatggccg gcgggttggg gttgatgtat ctgttcaagg aacgggcggt gaatcggggc
    37201 agtcagtccc gtttctatgc ggcctttcca gacctcgaaa caatcgatga actcgggtat
    37261 aaggccatta tgttgggctt cccgctactg gctttcggca tcattctcgg ggccatgtgg
    37321 gccaactacg cctggggcgg ttactggagt tgggatccaa aagagacctg gtcgttgatc
    37381 gtatggctca tttacggcgc ttatatccat gcccgcatga cgcgtgggtg ggaagggcat
    37441 aaggcggcca tctacgcgat cttcgggttt ctgatggtga tcttctgttt ctggggagtg
    37501 aattttctgc tctctggtct tcatgcgtac gcgtagacca tgagtgatat tctgaatcaa
    37561 agttcaagcg gcgttacgac acgaagtggt ccttccagaa cggtcgtggt gctggctgcc
    37621 gcagccattc ttggcgtgat gtttctcgtc gtctggctgc agagctcaaa gtatgagccg
    37681 ttgatcgtcg gaaaagaggc ccccgacttt cagctacctg acttgaacga caagccctta
    37741 cgcttgtctg attatcgggg gaaagtcgtc tttttgaatt tttgggccac ctggtgcaaa
    37801 ccctgccggg aagaaatgcc ttccatggaa gtgctgtaca agaattttga aaaggacgga
    37861 ctcgtgattc tggcagtcag catcgaccgg gtcacgacta agaaagagat cccgcccttt
    37921 gtcaaaggcc tgaatctgac ctttcccgtc ctggtcgact cctggggaca aacggataaa
    37981 cgctacaaat tgatgggagt tcctgaaacc tatatcatcg atcagcaagg tgtgttgcgc
    38041 gaaaaagtga ttggtcctcg ggattggacg cgattagata atctcaaagt cctgacgcaa
    38101 ttgctcaagc ctgctgaaaa ggcggcaagt gcagccactg ttgaagcagt cccagcgcta
    38161 taggcggatg atgaaccgtt ggatgatgct ctccataatt gtcggcggat tgatgatagg
    38221 cggagtgatg attgccgacg ctccatgggc ctcgccccta ttgagcccag ccccagctcc
    38281 aattctggtg gcgagccctg cgccgcaggt tgatgaagag gctccgaact ttctactgaa
    38341 ggattccgag gggttcaatg tcactttgga ccagtttcgc ggcaaggtcg tgcttctgaa
    38401 tttttgggcc acgtggtgtg gtccctgcaa aatcgaaatg ccggcgatgg aacggcttta
    38461 tcgatcgttt agccggaagg agttcgagat tctggcggtt tcaaccgatg cccaaggcac
    38521 ggccgtcacc cgcccctttc agcaggaagt cggctttaca tttcccgtcc ttcacgacgc
    38581 cgatttcaga atcggacttc agtatggcgc ccgacagctg ccgatgacat ttttgatcga
    38641 tcgcaaaggt atcattcgga atcgaattcc cggcgctcga gactggagcg cgccagcggg
    38701 gaaacggttg gtcgaagcgc ttttgcaagg gtcctagtat gaccgactca gtgcaatcga
    38761 tttccttggt ggccgctttc tcagccggcc ttctctcatt tgtctctccc tgcgtcctgc
    38821 cgctggtccc gtcctacatt tcctacatca cgggcctttc gatcgagcaa ctaaccgacg
    38881 tttcagagcg aagccgtttt cgcaaggcca tcattttgaa ctccttgctg tttatcgcag
    38941 ggttctcaac ggtatttgtg gcgttcggag cttcggcgag tctgttgggc caggtgttga
    39001 ttacctatca ggagcatttg cggcgtttcg ggggcattgt cgtcattgtg tttgggcttt
    39061 atctcctggg cattctgaat ttgaacttcc tgaaaatgga acaccggtat caattccgga
    39121 atcgtcctgc aggctttctc ggatcattct tgatcggcat tgcatttgcg gcgggttgga
    39181 cgccgtgcgt cgggcctgtg ctcggaacaa tccttctgta tgcgagcacg accgaatcga
    39241 tgttcagtgg agtcgtgtta ctcacgttct attccctggg attagccttg ccgttgttcc
    39301 tcacggcgct aggggtcgat cggttcctga gctacttcaa ggaagtgcgc tcctatcttt
    39361 ggggagtctc cacggtgagc ggggtgttgc tcattgtcgt gggcgtgatg atctatggaa
    39421 attcgttgac catgattacg agctttctcg agcgatacgg gattggctgg tatcttggtc
    39481 aataatcagg cctcatgagt gcttccctct ccggctcttc ctgacctctc ccacgcagca
    39541 acaatttctc acgatggtcg ccgaagactt gcgcggcaag ccctttcaca accggtgtgt
    39601 aaccatggct gatggatacg gacccacaac gtacgatgtg atcattgtcg ggatggggcc
    39661 ggccggatcg acggctgctg cggctcttag tcgaggcggt ttgacggtgc ttggtttaga
    39721 taaggatgcc cacccccggt acaaagtgtg tggcggcggc ttgtcggcgc gtattgatca
    39781 cgtgctggaa cccggttttc actccgtggt cgagcagtct gtgagtggtg tgcagtttgt
    39841 ctatcggggg caagaccctc tgctgatcga atcagcgcgg ccgattgcct acatggtcat
    39901 gcgggaccgg ttcgatcact atctcgtgca ggaagcgatt caggccggcg cgcatatccg
    39961 gaccggccac ggcgtggtgg aactcatgca ggatcaacat ggcgtggaag ttgtcacagc
    40021 acagggacgc tatcgggcgc agatcgtcgt cggcgcagat ggcgcgaata gtgtcgtggc
    40081 caggcggttt ttcccacaac gatctcagta ccgggctccc gcattggaaa gcgaagttcc
    40141 ccttggcaat ggcagacacg gtcattctgc gtcaacgatt ctcgtagatg tgggcgcggc
    40201 acgtcagggg tatggatgga tattcccgaa gagagcatgc ctttctgtag gagtcggaga
    40261 gtttcgccgg aaatcgaaga gcctgcgcgc cacctttgac cgctttgtca cggatgagca
    40321 acacgtgatc gggggggcgg tgcctcgtcc ggcagggcat ccgattcctg tgtatacaga
    40381 atctgaaaag cgggagcagg acgaagagtc ccgttttgtc gcaggccgcg cggcactcgt
    40441 cggtgacgcg gcccatctgg tcgacccgtt gtttggcgag gggatctact acgcgatccg
    40501 ttcaggacaa ctggcggctc atgcgatact ggcccatcga gatgaccacc acatggcggt
    40561 gtctgcctac gcggctgctc tgcggcggga aatccttccg gactttcgcg tgacggccag
    40621 aatggctcag gttatctatg cgttcccacg cctggccttc aagcttttgg ggcgttatca
    40681 ggatgtcgtt caggattatt tcgaagtgct gcaggggcgt acgacggctc agcaattcct
    40741 ccccaacgcg aagaagcgcc tgaaggcgtc agtcaacgac ctcctcctcg aagcattgca
    40801 tctgcgatag ggtagtgagc caggtaagcg ctaggtcagc accaggctcc gagccggcat
    40861 gagacgaagg gctgggacaa ggcagcactc ggcgcccgtt tgagctcggg gacggcgggc
    40921 gcagcaaagg tcgatgggac ggaggccgcg aggagcgtgt tagatggtgg gctctgtgga
    40981 gccggcgttt ctttgctggc cacgatggcc gccggcggcg acttcttctc tagtttggcc
    41041 agtaatcgac ggccgcttcc cgccacatca ctattcggat atttttcggc taagagctgg
    41101 agtttttcca tcgcccaatc gttcgcgccg agctcctggt acgtcaacgc cagatagtat
    41161 aaggcttccg gggctaccgt cttgtccgga taatccttca tgatcatgtc gaaccgatgg
    41221 gccgcagcta gatacgacga gcgacgatag taaaattgcc caacaaacag atgcgtcttc
    41281 gcgagccaat catggcagtc agcgagacgc tggagcgcct gggcctcgta tttgctgttg
    41341 gggtagtcct tccgcaattt ttcaaatgcg gtaatggctt tctgaatggg atcgggatcg
    41401 cggtcaatcg attttcccat ccgaagatgg ctttccgcta agcgaaattg ggcatagacg
    41461 gcaagctggt ggctacgatg caattccagg aaatgctggt attcaacgat ggcttcggcg
    41521 aactcttcct tgtcgaaaaa ggcctcgccg cgcttcatga tgacgttggg atcgtaattt
    41581 ttctcgatgg tgtcgccaag aaagatttgc tcatctgttc cgctcacggg tttgccttgg
    41641 gccgtagttt tgggtttgct ggaacacccg gccgaaaaac acagcagtcc aaccatgacg
    41701 gcgatgcgca cacaccaatc cctcgaatgc cgatgcatcc agactccttc gcacagcaag
    41761 cattgatggt agagacaacg taactacaca atttttctag cagatcgtga aatgaaaagc
    41821 aacgaaatca atgtccggag ttgaccctcc tgaagccggg tcctataatc gcgccgcacc
    41881 tctgcatggg gtgcgacgcg ttgtaaaacc aaatcccaga aggctccagt aggaattccg
    41941 tgaagtgcag tcggattgtc gggaccggtt cctttgtgcc ccctcgagtg gtccataacg
    42001 aggaggtcgg gaagccgctt gggctgaatt cggatcagat cacggccctg acgggtattc
    42061 gcacgagaca ttgggccgaa ccggggcagg catcatcgga tctggcggtc gaggcaggac
    42121 ggcgggcctg cgaggctgcc ggaataccgc cgcaatccct gagcgcgatt ctcgtttcga
    42181 ccacctcacc ggacagcgcc tttccttcca ccgcctgcca cgtgcagcgt gccctggggg
    42241 cggagtcggt catggcgttc gacctatcgg cttcgtgttc ggggtttctc tacgggttgt
    42301 cgatggccga cgcgatgatc cggagcggtc acatccgatc ctgcttggtc gttgcggccg
    42361 aagtgaagtc ccgctcgctg gatccggcgg acagagaaac agccgtcttg ttcggcgatg
    42421 gggcgggtgc cgtggtgctg gtgcaggaag aatccattcg cccgcaggcc gcgggcattc
    42481 ttggcgtgcg cctgtatgca gaaggggcag ggcatggcct catcaccatt cctgctggag
    42541 ggtcgcggaa gccgagcggg acggagaccg tccgtggaaa agatcacctc ctgcggatgc
    42601 gggggagtgc cgtgtttcgc gccgcgatcc gtcatttgga acaggcgatg ctcgaactgt
    42661 tgaaggaatt tgggctctcg accgttgatg tgaggagggt cattgctcac caagccaacg
    42721 ccaggatttt ggagcaactt cgtcgccgtc tcgggctacc ccgagaggtg ctctattcgg
    42781 tcattgaacg ctatggcaat acttcgtccg cgtcccttcc catcgcgtta gactgtgcgg
    42841 ttcgagagca gaggattgca gctggagatg tcgtagtatt ggcagcattt ggcgggggct
    42901 taacctgggc cacaggactg gtgcgttggt aacccctcgc ggctagaaag gacgttatgt
    42961 tcggtctcat accccgggaa gaagcgtttt tcgatttgtt caaaaatgcg gcgcataaca
    43021 tgatcgaagg cagccgtctt ttgaaggaca tgacggaaga ctttcggaat ccggttgaga
    43081 aagccaaacg gattaaggat gtcgaacatg tcggggacgg catcactcat gacatcgccc
    43141 ggaaactgaa ccagaccttc attactccca ttgatcggga agacatccat gatttggcca
    43201 gtgcgctgga cgacattctg gatgtggtcg aggcaatcgc cgatcggttc gtcttgtata
    43261 aggtcgtcgc tccaacggag gcggcgatca gattggccaa tgtcctgtat caggcggcgg
    43321 ttgaggttgg agccacggtg gacttactcg gcaagtccca tcaggcggtc acggagtgca
    43381 gcgtgcgggt gaatagcctg gaaaacgaag ccgatcgtat ttcgcgcgat gccatctcgt
    43441 ctttattcga gaaggagacg gatccgatcg ccgtcatcaa gtggaaagaa atctacgaaa
    43501 atttcgaggc gggcaccgat cggtgcgaag atgtcgcgaa cattctcgag cgcatcgcct
    43561 tgaagcacac gtgagcaacc ctcgcacatt cttcgatact catcctgacc aatacacgag
    43621 ggataggacg cggcattcat ggctgaactg agcggattgt tgttagtcgt agtgattctg
    43681 gcgctgctgt ttgacttttc caacgggtgg catgacagcg ccaatgctat cgcgacggtg
    43741 gtctcgacgc gcgtggtcag tccttctacg gcggtccttg tcgccggtgc gctcaacgtg
    43801 gccggagcgt tcatgtccac tgccgtggcc aagatggtcg gcacgggaat tgtcgatcct
    43861 caatcggtga cccagaccgt tgtcgcctct gcgctggccg gtgccatcgt gtggaatttc
    43921 atgaccctca tgctgggtct cccgaccagc tcctcccatg ccctcatcgg cgggttggta
    43981 ggcgccgcgc tgacccatgg cgggatggcg gccgtcaaat tttcaggatt acgctcggtg
    44041 ctggaggcga tgatcctgtc gccgtttttc gggtttgcga tcgggctggt gttgatgatc
    44101 gtattgagct ggctcttttt tcgtgtgcca cgagccttgg ccttgagatt gttttcgcgg
    44161 ctccaactcg tctccgcaag ttttatggct ttcagtcatg gggcgaatga tgcacagaaa
    44221 gccatgggca tcatcacgct ggcgctcgtt tcagccgggg cccttcccaa ggcggaagtg
    44281 ccgacctggg tcatcggatc ctgcgctctt gcaatggggc ttggcacggc ggtgggcgga
    44341 tggcgaatcg tgcgaacgtt ggggatgcgg atcgtgaagc tggaaccggt ccatggcttt
    44401 gcggcggaaa ccggagctgc gatcgtgttg atggccacgg cgcatatcgg acttccggtc
    44461 agtacaaccc acaccattac ttcgacggtg atgggagtcg gagcggtcaa gcgggtctcg
    44521 gcggtgcgct ggggtgtgac ccgtcgcatt ctgtcggcct ggctcttcac cctccccgga
    44581 gctgcgctcc tgtcgactat atgttacctg ctgctgtcga aattgtcgta gacgactcct
    44641 gcgcaacggg caggtgaacc acaaaccggc tgcccctggg cgcatttccc tccacccaga
    44701 catgcccgct atgggcttcg acgatgtgtt tgacaatggc gagtcctaaa cccgttccac
    44761 ccaattcgcg cgagcgggcc ttatcgaccc tgtagaatcg ttcgaagacc ctcggccggt
    44821 ccgcttcggg aatgccgatt cccgagtctg ccacgacaat ctcaatcatc ggacgcggtg
    44881 acgccgtccg gctttgcgtc gcctggcgga ttgccataga gatccggccc tgatccggcg
    44941 tgtatttgac cgcattttcc agcaggttaa taaagacctg aacgaggcgt tcctcgtccc
    45001 cttccaccac gacatactcg tccggaagcg acaattcgat cgtatgatgt ttcttgtccg
    45061 ccaacggctt gatcaccgca accgtccgtt ccagcaaggc ccgcaattcc accggctccc
    45121 tgcgaaacag tacctggccg gattcgatct gcgacaattg cagcagatcg tcgaggatga
    45181 ggttcaaacg attgctttgc cgcatgatga tttctaaaaa cgctgctgct gtcgaagggt
    45241 cgtccttgcc gccgtccagc agggcctcga cgtacccctt gatcgacgtg agcggggtac
    45301 ggagctcgtg cgacacgttg gcgacaaaat ctttgcggat tttctccaat cgacgcagtt
    45361 cggtaatgtc atggaagaca aagacggcac aggcttcctg ctcacgattg ccgccggcga
    45421 tcgacgcttc cacccgcaaa cagctgccgc tgggggagag cgtaatttca ccgccctgac
    45481 cggatcgcgt ctgcaacacg gccgagacca gcgcggtcag cccttcgtgc cggatcagtt
    45541 cggcataatg acggccgcgt gattccgtca gctctaatgc gaacatccgc tccaacgccg
    45601 gattcacctg aacaaccgtg cctcgatagt ccagcaccat gacgccctcg accatcgcaa
    45661 tcagcatggc caggagttgc gcgcggtcgt cggagacttc cttgatcttc gtctccagct
    45721 ggtcggtcat ctggttgagt gttcgcgcca gcaaaccgac ttcatctgat gacgagacgg
    45781 tgaggcggat cccgggcgtc cccgcggcga gttggcgagc ggcagcagcc atctccgaga
    45841 gcggctttgt gagattgcgt gacacccaga ggctgagcac catcgccagg agaaaggcgg
    45901 ccccgaacgc tgtcagcaag tcctgctgaa tgtgccgtac ccgttcatcg atggaggtta
    45961 atggcaggcc gaggcggatc accgggacgc ctggttgtat gcgcgccggt tcggaaagga
    46021 gccgggcgac atagtacgtg cgctctccgg tagtctggct ggctcgaata tccatgcccc
    46081 ggccggatgc cagcgcttgg gccacttccg ggcgggcaag atgattgtcg atgtgagtga
    46141 gtccttcggc ggggaccgcg ctgtccgaga gcacggtccc gtcctgtttg atgacggtga
    46201 tgcgaagacg ggcctgctgg ctgagttcgc ggatggtggt gtgaagcagg gggctgggaa
    46261 cggcttgccc ggtttgatca aacagcggcc tgagcgccat ggcggccaag ctgctgcgcg
    46321 tctccagcat ttccgccatg cggctgagtt cggtctgttc caccgaacgg atcacgagcc
    46381 agcccgcgac caggaggccg acaaggacgg cggcgagtgt gcccagggcg actttccacc
    46441 gtaggctgag gttcatgccg gaatatcaga ctcgcgcagt ttgtagccca gtgatttgat
    46501 cgagatgatg gcgtcatcca gaagcgggag tttctgtttg agccggcgca cgtgaacatc
    46561 caccgtgcga gtggtcccat aataatcata tccccacacc gcgctgagca ggacgtcgcg
    46621 cgtgagcacc cgcccgacat tgcggagcag atgctccaag aggccgaact cttttgcggt
    46681 gagcgtcacc tcttctccct ccagccggac ttcatgccgc gacagatcta cggtcaaggg
    46741 gccataatga tattgggtct gaggactgtt ggcgttgcga tccagtcgcc ggagtaatgc
    46801 cttgacccgc gccaccagag ctttgggact gaaaggtttg gtcacgtaat catcagcacc
    46861 cagctcaagc ccgatcacgg tatcggactc ttcggccttg gcggtcagca tgaggatggg
    46921 gagaagtgcg gtctcctggt tcagacggat ccgtttgcac acctccagcc cgtccagttc
    46981 gggcaacatg agatcgagaa taatcagatc gggatgttcg gccttcacct gccgaaggcc
    47041 ttccgtgccg gtcatggctg acaccgtccg gaatccctcc ttctcgaggt agagtttgac
    47101 tagctggaga atatctttct catcttcaac gatcagaatt tttttgtgcg tgctggaagg
    47161 tgatgacatg gccggattgt tgcacaatgg ggcaacggcc tcaactgctt ttggggctgg
    47221 caagatgcgg atgacgcggt tgacgtcaag ggcgggtctg acgtaaggtg accgcttagt
    47281 gagacgatgt gtataccccg gcaagccggg cagcccttca tgatcgcgag agggaatcag
    47341 cagatgaaga tatacttagc caatcctcgc ggattctgcg cgggtgtcga ccgcgcgatc
    47401 gatattgtcg atctgtccct gaaaaaatac ggcgcgccga tctatgtgcg ccatgagatt
    47461 gttcatagcc ggcatgtggt gaactcgctc cggcacaagg gtgccgtatt cgtggaggag
    47521 ttaaatgagg tgccggaagg atcagtggtg atcttcagtg cccacggtgt ggcgaagtcg
    47581 gtatgggaag aggcgcagag ccgccgcctg catgtcatcg acgccacctg tcccttggtg
    47641 atcaaggtgc ataacgaagt caaccgtgat tacacgcaag gctatgaatt gattctgatc
    47701 ggccatgctg ggcaccccga ggtgattggg acgttggggc agattccgga taagttccat
    47761 ctcgtatcct ccgtgcagga tgtcgaaacg ctccacgtgg aaaagacgca gaacctgtcc
    47821 tatgtcaccc agacgacact cagtgtggat gagtgtcgag atatcgtcga ggccttacat
    47881 cggcgattcc ccaatatcaa aggcccgcat caggaagata tttgttacgc gacgcagaac
    47941 cgtcagaatg cggtgaaggc cttgtctaag ctcgtcgatg tcatccttgt catcgggtcg
    48001 ccgaacagct ccaattcgaa ccggttgcgc gaattgggcg agcattgcgg cattccctcc
    48061 tatctcatcg atgccgcgtc ggatatcaat ccggactggt tgaaggatgc caagagtgtg
    48121 ggcctgtcgg caggggcctc tgcgccagaa gtgttggtga cggaagtggt ggcctatctc
    48181 aagcgtctgg gttcttctga agaagtggaa gaattgaccg tcattgaaga ggatgtggaa
    48241 ttcctcctgc cgaaagaact cgtcaccatt gaatctgcat cacgcacggc cgcctctgcg
    48301 aactaactgc tcggccgtcc tcatattgtc ctacaggtag atcaggccat tcctccatat
    48361 attgggggag tggcctcttt attcccccat atcaagggct tttcctttgc attgattcta
    48421 tcgctgtgat agaaggtttt gcgttttctt cctcacaacg cgtcccttct caaccttctt
    48481 gctctggagg tcgccggtgt atctgaagtc cttggaaatg ctcggcttca agtcattcgc
    48541 ggaagcgaaa attcaatttc ccaaaggcat tacggcgatc gtaggtccga acggcagcgg
    48601 caaaagtaat gtggtggatt ccatcctctg ggtgttgggc gaacaaagca ccaaaacgct
    48661 tcggagcgag aagatggaag acgtcatctt caacgggacg gaagtgcgga aaccactcgg
    48721 gttggtggaa gtctcgctgg tgatcggggg actaggcgag ttgcgattgg atgcgatttc
    48781 aggattgccg agtcaattga gcgaatacca ggaactgatg attacgcgcc gtctctatcg
    48841 caatggcgac agcgagtatc tcatcaacaa gacgccctgc aggctcaaag atattcgcaa
    48901 tgtgctgatt gaaaccagag cggggtcaaa gggccatact gtcatcgaac agggacgcat
    48961 tgagcagatt ctgcaggcgt caccgcagga ccgtcgggaa ttgatcgaag aaaccgcggg
    49021 catcgtccgg tacaaaaaac agaaggctga agcgcttcgt aagctggaat ccacgcaaca
    49081 aaatctcctg cgggtgcgcg atattgtggc ggaagtgaag aagcagctga attcactgga
    49141 acgccaggct cgccaggccc ggacgtacca aacgctacag caggaagcca aggggctcga
    49201 gatcgagttg ttgtcccgtg actatcgcac catgcatgcc gacctggaat ccgtcgaccg
    49261 cgaagcgcgg gaggtggaag cactcgaggc cgagcaggtc gccgagcagg ctcgtctgga
    49321 ttcggagcag gaagccatcc ggctccggat gaacgacacg gccgaggcca tctcgcgcgt
    49381 gcgggatacc ttggccggga ccgagcagcg gcaatcgcag gcactgacgg ccgcggaggt
    49441 ggagcggaac cgcacggaat tgttcgagcg gcaacgcgtg caggcttcgc aggaaatgga
    49501 gcgactggcg ggggatcagg agcaggcgca ggttgaaatc gaagacctgc gagcgacctt
    49561 gctgcaactg gaaggcgaca ttgtcgagca agaggcgcag tttcagcagg cggatgctga
    49621 agcgaaagcc ctggcggggc atcgggccgc cgctgtcagc gaagaggagc gcgcccggaa
    49681 ggacgtgctg aacctcgccg tgctcgtagc gaacacggag caaagtctcg cgcaggtgac
    49741 cgcgcgccag caggaaacgt cagcccgggc ggaacgggtc tcgcgtgagc aggagcagat
    49801 ggtcgcgcaa gtggctggat tggatgagca gcgggaactt ctggcggcgc agcgggaaga
    49861 ggccagtcag cggatccagg gactcatggc ggaacggcag gctgcgatcg agcgtattga
    49921 agggctcggc gggcagatca cgggcttgga tcgcgatatc gtccgatttt ccgaagatgt
    49981 ggctgcagtg gaatcgcgcc tgggcgcgtt gcagggtgtg gtgcgggagg aaatgggcta
    50041 cggccgtgag ggagaggaag aaggcaccgc gctgaaaacc tgcgacggtg tgcgcgaggc
    50101 gcttgcagag tggctcgtca ttccgtccgg gctggatcgt gcggtggaga cgattctggg
    50161 tgagcgggta cggggctggt tggtcgatca gccctcagcc gccagccgag cggtggagtt
    50221 tttgaaaggt aaggaacttg ggcgtggcgc atttatccct cagcagatcc gttgggcgac
    50281 cgaccaggca gcagcggatt cttcagcttc ctggtggcct gcactcaatg ggcagccggg
    50341 agtcgtgggg cgtgccatcg atgtcatccg cgcagaaggc gcatccgctt ccaccttgag
    50401 ttatttgttt gatggcatcg tgattgtcga atccctcgct gtggcactgc aattgtggga
    50461 acagcatcag tggccggcac ctgcaggccc aacctatgtg accttgtccg gcgagacgtt
    50521 ggatgcagcc ggcgtcatga ccgggggtgg gaccagtgcc ggtgcgggac tcctgcagcg
    50581 ccggcgggaa gtgctggagt tggaggctcg ccgcatggaa gcggtgcaag cgctggaact
    50641 cgctcgtgtc gcacgtgagg aagcagcctc cgcattgggt ctggggcgtg aggaggaaca
    50701 gcagctgggc agggccatcc gcgaggccga gatgctggag ttatcccttc agaaagacga
    50761 tgcaaacgtg gagcgccagc gtggagagtt gcaccggcgg atggacatgt tggccgaaga
    50821 gctgcagcgg ggattggctg aacaggcgcg gttgcaggag cagttggaat ccagccaggc
    50881 tcaacttgag caatgggtgg cagagaagct cggccaggaa aacgggttgc ttcagatccg
    50941 tgaacgactg gtggtcatcg agagccaagg gctggcgatc cagcaacggc tgaccgaagt
    51001 gcggttgtcg ctcgaaagca tgcgtggacg tcgcgagcat gctcacagcg atattgcgcg
    51061 gttcacgcaa cggctggatg ctgcgcagcg acgtgcggcg gagctcgagg agcaagtgac
    51121 cggactggta caggctacgg agagcagccg cgaggagcag accaggcagg aggcgctctg
    51181 ccgtgaactc ggtgccgagg tggatggcat caaatcggaa ctcatcgcgg cgcaggaacg
    51241 tcaggctgaa gaaatgagcg gattgcatac cgtcgaagaa accctctcca ccgtgcggca
    51301 gagtttgtcg gccttgcatg atcgccgcat gacggctgaa gtgcgcaagg ccgaagtgaa
    51361 ggcgcatctc tcgaccatcg agagcacgct ggccggcacc tatcaaatcg atccggctac
    51421 tctgctggtg gtatcaactg aggaacagcc cgtaactgaa ggagaagagc cagctgcacc
    51481 ggtctctctg ctcgagacgc cacagttgcg agaacagatt cagaaaatcc gggaacggtt
    51541 ggaccgcatg ggcgcgatca atctcgcggc gatcgacgaa catcgtgaat tggaagaacg
    51601 gtatcagttt ttgacgacac aagagcagga tctgtctacc tctattgcct cgctcaagga
    51661 aattattcag aagatcaacc ggaccaccaa agacatgttc gtggagacct tcaacgaatt
    51721 gcagcagaaa ttccgggatg tgttcgcaca attctttccc ggtggccgcg cagagctgca
    51781 gttggtcgaa gagccgttgg aagaaggggt ggaggataac ggcgcgcgcg agccgggtgt
    51841 ggaaatcgtg gcgcaaccgc cggggaaacg attgaagagt attaccatgt tgtccggcgg
    51901 agaaaagact ttgacggcga tggcgctgtt gattgccagc ttcttgatcc gtccgacgcc
    51961 gttctgcatc ctggacgaaa tcgatgcgcc gctggacgaa gagaacatcg ggcggttcac
    52021 aagcgtcttg cgcagcctgt cctcgactgc ccagttcatg gtcatcacgc acaacaagcg
    52081 gacgatggcg atggcggatt cgctcttcgg cgtcacaatg gaagagcccg gcgtgtcgac
    52141 cttgatctcc gtcaagctcg gcgatttgca gccggcctaa cgaggacggt gtcggggaag
    52201 cagcgcgctg agtcttctgc tcaaacgcgc acttagcgcg cacttcgagg gctacttccc
    52261 ttgactggtc tgaagcagtt tgttatacag tcctgctaat ggttatacat ggtactgtgc
    52321 ggtttcatgt gatcactgct tgattgatgg aaccgtttcg actatcgatc ccgccgtctc
    52381 tgtctgattc acggcaacac gctatgcgac ttctcaagaa tctcttcagt cgtctgtctg
    52441 atagtctgct ggtctcggtg ccgagccgaa tcagagcggc gcgcgatttt gcctctgctc
    52501 ccgtgctgcg cctcgtctcc agtaagcccg atgctccagc cagcgacggt gcaaagcgag
    52561 cgccttcaca caccaccggt caactcgaag cactggaaga agccatccgg aagagtcagg
    52621 cgtggtttct ggcccggcag gatgctaccg aaggtttttg ggtagctgag ctggaagcag
    52681 atacgaccct gagctccgaa tatctgatgc tgcggcggtt catgaactgc gtcgacccgg
    52741 agcgcgagcg caaagtcatc cgctatttga agtccgccca acttccgtgt ggaggctggc
    52801 ctatttatca cggggggccg gcggatatca gcgcttccgt gaaagcgtac tttgccttga
    52861 agctttccgg cgtctcagcc gatgaaccgt tcatggccca ggcgcgaacc tgcatcctcg
    52921 ataagggcgg ggtcgtcgcg gccaatgtgt tcacaaaaat cgccctcgct ttattcggtc
    52981 agtatgattg gcgcggcgtg cccagcatgc cgccggaaat catgctgttg ccgaagcggt
    53041 tctatttcag catctacgcc atttcttatt ggtcgcgcgc ggtcctcatt ccgctcttga
    53101 tcatttttgc caaacggccc cagtgttatg tgccacccga acagggcatc gacgagctgt
    53161 acacgcagcc cccggcggag attgattaca gcacggtccc tccattcaag aaggaccgga
    53221 cctggtttac ggccagaaac tttttcatca atctcgacgc gctgctcaag gtctatgatc
    53281 gctcaccggt cgagtgggtc cgccagaagg cgctcaaggg cgccgagaac tggatgctcg
    53341 atcatatgaa ggggagcggt ggactcggcg cgatttatcc cgccatggcc aattcggtga
    53401 tggctctgaa ttgcctcggc tacaagaatg atgatcccct cgtcgtcaaa gcgatgcggg
    53461 agatcgaaga actcgaaatt cacgatacgg tgatggacaa cggacaatgt gtcgatgcgc
    53521 tgcatctgca accctgccat tctccgattt gggacaccgc cttgctcatc aatgcgctgt
    53581 ccgaggccgg tatgtcgcag gatcatccgg cgttgcagaa agccgccacc tggttgttat
    53641 ccaaacaaac aaagaccgtg ggcgactgga ttatttcctc ccccggcgcg gaaccgggcg
    53701 gctggtactt tcagtttgag aacgagctct tccccgacgt ggatgattcg gctgtcgtgc
    53761 tgatggcgct tgctaaagtg cgtctgcctg atgaagacca gcagcggctg gcgattcgcc
    53821 gagggtgccg gtgggtgacg gcgatgcagg gatccgacgg cggctggggc gcgtacgacg
    53881 tcgacaataa tcgaatcgtg ttcaactaca ttccttttgc cgaccatcgc gcattgttgg
    53941 atcccagtac ggccgacctg gccggacggt gtctcgaaat gctcgccacc ctgggatatg
    54001 actggacgca tccgtccgtg gcctcggcgt tgacctttgt gaagaacgat caagagcagg
    54061 acggtagttg gtatggtcgt tggggcgtca attacattta cgggacctgg tcggtgctct
    54121 ccgggctgcg cgccatcggc gaggacctgt cctctcccta tatccgtcgc gcggtcagct
    54181 gggtcgagtc gaagcaaaat cccgacggtg gatggggcga gtcctgtctc tcctatgcgg
    54241 atgtggcgga gagcgggcgt ggcgatagta ccccgtcgca aactgcctgg gcgctcttgg
    54301 ccctgatggc aggcggcgtc acagactcat tcagccttgc ccgaggtatt cactacctca
    54361 ttcgaaatca gcggaaggac gggtcatggg aagaggtgcg ccataccgga accggtttcc
    54421 ctcgcgtgtt ctacctccgt tatcattggt attgccaata cttccctctc tgggcgctgg
    54481 ccatgtatcg caatctgaag actcgtggaa cgacgagggc ggatgaattg cgacagctgg
    54541 cctatcaatc aggacagttc cggtcgcctc gctgaccgtg gatgctcatc gagtccattt
    54601 cccctcgcca tcagttatcg ggagccgctg tgaccacgat cggtgtcttt gttgccaccc
    54661 ggtgggaatt ggccgccgtg cggcacgcct ttcctgcgag cgaagtccgg gccgtcggtg
    54721 gaattcgttg tgtggtggta cgccagccgt tcgtcgaatg gtgcatcatt cccatgggag
    54781 tgggtccgga gcgggcgatg gccacggcca aacggatact cgcggaacag gcgctgtcgg
    54841 ccatctggtc gacgggattt gcctgtgctc tgggaccggc cggcatcggg gacgtgttga
    54901 ttggtacgga cgtgatcctg gaaaacggcg ggaaggcggg gccttctgtg ccgtgcgcgc
    54961 cggcgatggt cgagtgggtg aggcaggcgg tccatgtgca aggcttgccg atgcgaagcg
    55021 gagggtttgt cacggtgccg cgtgtactct gcctggcgga agaaaaacgc gcgatcgccg
    55081 gacgagccgg aggcatcggt ctcgacatgg agagcgcggc cttagggagg gtggcctcgg
    55141 agcatcgaat cccttttgcg atcgtccgca cggtctccga tctcgaggat gagagtctgc
    55201 cgcttgactt caacctgttt ctcaggccat ctggatgggc gaagggtgtc gtcgcgtgcc
    55261 tggcacatcc gacgagcctg atcgggctca atcgtctccg agtgcagagt cagattgccg
    55321 gcgcacagtt gacggcgttg tttcgtgcct gtgccgatcg tgctctgggt gaaggcatcg
    55381 gctgatcagg agttatgcgt ggcggtcgtc cggcccgtga gagaagagga gggtgaggcg
    55441 tggaaggtat tgctcgcatc ggacggttta ccatcgatct gacggagcag atggggcgaa
    55501 tgatgctgtt cgttctctcc tcctttgcct ggctgacccg tccgccgttc aggttctatc
    55561 aaatcgtcaa acaactcaat ttcatcggct ataaatccac cttcgtggtg gtcctcaccg
    55621 ccgtctttac cggcatggtt ttggcgctgc agggttacta cacgctgcgg aaattcggct
    55681 ccgaggcggt gctgggatcc gccgttgcgc tcagtatcat tcgtgagttg gggccggtgc
    55741 ttgccgcgct gatggtgacg gcccgcgcag gatcggctat gacggctgaa atcggcatca
    55801 tgcggattac cgagcagatc gatgctctgg atacgatggc catcaacccc ctgcagtatc
    55861 tgattggtcc caagctggtg gccagtttga ttgctgtgcc gttattggtc gcactgttcg
    55921 acgtcgtcgg catttacggt ggatacgtcg tcggcgtgca actgctgaac ggcaatgaag
    55981 gcgcctattg gagttcaatc gagtcggctg tggaatggaa ggatgtctat ggcggcattc
    56041 tcaaatccat cagcttcggc ctgttgatca gttgggtctg ctgttacaag ggctttcaca
    56101 cgaaacacag tgccgaaggt ttgggaacgg ccacgaccga agcggtggtg ctgtctgccg
    56161 tgttgatcct ggtgtgggat tactttctga cctcggtgtt gctatagagc tgtcagtcat
    56221 cggccgtccg cattcagcgt ttggatgtga acggattccc gaacgctgat gacataaatt
    56281 gctggattca gatgattaaa ctggtcgggg tagaaaagac cttggggaaa caggccgtgc
    56341 tgcgcggcgt cgacctcacc attcccacgg ggaaattgac gacgatcatt gggcggagcg
    56401 gcgaaggcaa gagtgtgttg ttgaaacaca tcatcggcct catgcagccg gatcgcggcg
    56461 aagtctggat cgatgggacg gacattgccc gcctcaaagg acgggctctc aacgaggtgc
    56521 gcaagaaatt cgcgatgctg ttccagggcg ccgccctgtt cgattccatg acggtgtttg
    56581 agaatgtcgc ctttccgttg cgggaaaaac tccgcctgaa gggtgacgtc gtgaccaggc
    56641 gagttgagga gaagctggag caggtaggat tgaagggcat ggggcataaa ttccccgcgg
    56701 aactgagcgg cgggatgcgc aaacgggctg ggctggctcg tgcgttggta atggagccgg
    56761 agatcattct gttcgatgag ccgacgacgg gcctcgatcc gttgatggcg aaggccattc
    56821 acgacctcat cgtggcgatg cagcagcagt ttcgtttcac cgcggtgatg gtgagtcatg
    56881 agattccgga aatttttggc atttccgact atgtggcgat gctgaagaac ggccggattg
    56941 ccgagatggc accatcaagc gagtttgtga agacgaccga tgaagagatt cgcgagttta
    57001 tttttgtcgg gggcacggtc gcgcccaaag ggttgccggc tgagagtcgc tgataggaga
    57061 cgttatggaa cgtgcaaagc tggaattgat ggtcgggatt ttcgtgctcg tcgggatcgc
    57121 ctgcctggga tatctttcca tcaagctggg aaaactggag gtcatcggcg gacacaatta
    57181 tccgatcgaa gcggagttta cgtcggcctc aggactgaag ccgggagcgt cggtggagat
    57241 tgccggggtc gaggtgggcc gcgttcgcac cattggcctc aatagtgacc gggcattggt
    57301 ggccctggcg attcaggatg gcgtcaagtt gtactccgac acgattgctt ccatcaaaac
    57361 ccgtggcatc atcggggata aatatctcgc actttccgta ggcgggggcg gcgatccatt
    57421 gaagcccggc gacaaaattc gtgacactga gtcagggctt gatctggagg aattggttag
    57481 ccagtatgta catggcaagg tgaactaatc tcaccggctc atttgcagag ggtggcaatg
    57541 cgaacattgg gctggtggga ccagggaatc accactcaca ggagaggtgt tggaatgcat
    57601 gtaaggcgat ggatggtcgt gggtgtcctg ttggcgctgc agctgttcgt gggcgggctg
    57661 tcgttcgccc tggccggtcc ggccacggaa tcgatccggg gaacagtgga tgaggtcctg
    57721 aggatcttga acgataaaga actgaaggcg ccggccaaac atgaagaccg gcggcagcgt
    57781 ttggaaaaag tcgtcgcggc gcgatttgac tactccgaaa tgtcacgacg atcattgggc
    57841 gcgcagtgga atcaactgtc ggacaaggag aagcaagagt ttgtcgatct gttccgaacg
    57901 ttgctgacca acacctacgc cgatcgggtg gagacctatt ccggcgaggg tgtgcaatac
    57961 ctcaatgagc gcaccgagaa agagtatgcg gaggtgcgca ccaaggtgtt gtccggcaaa
    58021 accgaaatcc ccatggacta ccggctgctg aacaaaagta atgattggca cgtgtatgac
    58081 gtggtggtcg atggcgtcag tctggtgaac aattaccgcg ggcaatttac gaagatcctt
    58141 cacacctcct cgtattccga cctcgttgat cagctccgca agaaatccga gaagatcaaa
    58201 gccccgtagt ctcaatagaa agcgaccgtt ccggcgagcg ccatgcagcg acattatttt
    58261 tcgtcgatcc gtattctcct ggcgctggcc ggacttatgt tcctgagctc tccggctccc
    58321 gcaggggccg atacccagat ttttccagtt ccttcggtct ccaccagcaa aaacgacggg
    58381 aacgacgccg gtttgatcgc acccatcctg atctccgatc cggatggcga attgaaatac
    58441 ctgatggcgc cgatgttgat tcagaattcg atcgtcggca gccgggcggt gttcaacctc
    58501 tttaagtacg atccgggggg gcggcagatg aagctgatcg cctccctgac agagaaaatc
    58561 gagcgcaagg tgttgtttga ctatgtcgac ccggcgttcg gtaacgggca gtattttctg
    58621 aacttcggtg gaaccttctt taaaaatgcc acgtcccggt tcttcgggct gggccaatcg
    58681 acggtccaag cggatgaatc gaattacacg gcgagagaag cgcgcgcgta ctggcgcctc
    58741 ggcctctatg cgaatgaggt aacgcaggtt tcggtggggc agcgggtgcg ccaggtacaa
    58801 ctccagcggg gcgccacaga cctgccgttt tcagtggagc aatttccgac tgtcgacggt
    58861 attcagggcg aatccatcat tgtcgggcat cgcgcctcat tttattacga tacccgcgac
    58921 agcctggtca cgcccaccga tggcatgtcc gtcatggcct atgctgagct gaatcaaaat
    58981 gtgaaaaatg gggatcatcc cgtgtattca cggtacgaaa tcgaagtgaa aaagttgttc
    59041 cccagcgaat ccaagcgtgc cattctggtc gtacgggccg atttgcaggc cacgatcggt
    59101 tcgcaagtgc cgttcttcga acagtcgtcg ctgggcggac aaaacaatct ccgcggattc
    59161 gggatggatc gctacatcga caaacatctg atcgccttca gtatcgaaga gcggattcat
    59221 atcttgcgca cgaagttggc aggggtgacg gcggattttg aactcgcccc gtttctggat
    59281 acgggccagg tgttcaattc ctttaaggat gtgagctttc aggattaccg gatgacaccg
    59341 ggtgtcggtt ttcgggcgat tgtgcgaccg aacgtggtcg gtcgcctgga ttacggatat
    59401 agccgcgaag gcggagcgat tttcgccggg ttggacttcc cttattaagc gcctgagtga
    59461 acggccgggt attactcatg cggcatctcg tctatttttt ctgcctcatc gggctttcct
    59521 gcggcctgcc gcttctggct ggcgcggagg ggtttggtcc cttcccggtc agaaactttc
    59581 aagcactgga ccagctggtg ttggccatgc ctggtgagcg ggcggctgtg ttgcgcaagg
    59641 gggatttgga tatccgtctc gaagcggcta ataccgccac gatcgcccgg gatcgggagg
    59701 agcaggccga tgtaaccatg aaatttgaga ccgtgcgcgc cggtctgttt cttcgatacg
    59761 gcctcaccga acgattggag gtgggggcgg agataccggc ataccatcgg tacagcggat
    59821 ttatggaagg tgcgattctc ggggtggaac gaggcacgac gggagtgtct cctccacgaa
    59881 aggctttgcg ggataccggg tacgccttca acgtgtccaa cggtggccgt acgatgttcc
    59941 agggatcgga aaatgccacc ggcttaggcg atgtctcatt ttatggcaaa taccagttgt
    60001 tgcaagagac cggaagccag ccggcattgt cgatccgtat ggcggtcaaa gctcccacgg
    60061 gagacaccgg acaggtattc ggaagcggcc acccggatgc cggcatcggt ctggccctcg
    60121 acaagacttt cgcgaccggc tggatcctgt acgccaacgt gaacggaatc tttcccacag
    60181 gacggatcgc gggacttgat gtgcaaccgg tcgtcagcgg gttggtcgcg gtggaatacc
    60241 tctggacgga gaatttttcc atcacggcgc agttcgacta ttattccccc ccgtttcacg
    60301 gcacaggaac ccgtgttttg gataaagggg tgaccgagtc ggttctgggg gtaagttacc
    60361 ggatactgcc gaggctgctg tggcaagtct atggagtcga aaacctggac ttcatcaccg
    60421 gcagcgccgc cgattttacc ctttctacgg ttcttacgta ccggtttcgg tcgtaaggca
    60481 atggtcgggt ggtccggagg acagcggaat cttgacacag gcgttgccat atgtgaggat
    60541 gaggcgattt gtacgaactt ctttcgctgc ttcgccacga ccttctcaag acgattgttt
    60601 tccacgaata tgaatacctg cgtccaattg tcatgctttg acaaggagat tgtgcaatgt
    60661 ctctgctaaa aaatatccac agtcccgctg atttgaagcg actgtcccct gaacagtttc
    60721 cggaactatg ccaggagatt cgagagcaaa tcctctcggt ggtttcgaat gtcggggggc
    60781 atctggcctc caatctgggt gtcgtggagt tgacggtcgc attgcaatat ctcttggaca
    60841 cgccgaaaga taagatcgtc tgggacacca gcaatcaggc ctacacccac aaactcctca
    60901 ccgggcgtcg ggaacagttc cacacattgc ggcaatacgg cggattgagt gggttctgca
    60961 agcgtgaaga aagtgcgtac gataccttca atgccggtca tgccggtacg ggtgtgtcgg
    61021 ccgcatttgg catggtggaa gcccgcgagc agcggggaga aaaacataag gtcgtctgcg
    61081 tcgtcggcga cggggcgatg acggcgggca tgacgctgga aggtttgcat cacgcgggcg
    61141 ggacgaacaa ggattttatt gtcgtcctca acgacaatca gatgtcgatt tcccgcaacg
    61201 tcggagctat ttccgcctac ttgaatcgga ccttcaccgg tgaattctac gcccggatgc
    61261 gtgaagaaac cggccagctc ctgcggaaga ttccccacat cggtgccgaa atgcaaaaaa
    61321 tcgctcgtcg ggccgaagag ttggccaagg gtgcgattct tcccggcctg ttgttcgagg
    61381 agttgggctt ccagtatgcc ggaccgatcg acggacataa ctttgagcac ctgcttccaa
    61441 cattggaaaa tgtgctgaaa atgaagggcc cggttctgct gcacgtgatc acgaaaaaag
    61501 gcttgggata tcaggctgcc atggataacc ccgtgtggtt ccatgcctgc ccgccgtttg
    61561 tgcgtgaaac gggggtgccg gcaaagaaag ccgtgcgtcc cagttacacc agcatggccg
    61621 tcgatgcctt gatcaaggtt gcgcgtcagg acaagcgggt ggttgcgatc actgcggcca
    61681 tgtgtgaggg cacggggctc aatgcgttcg aaaaagagtt cccggaacgc atctatgacg
    61741 tggggattgc tgagcaacat gccgtgacct ttgccgccgg tatggcggcg cagggcatga
    61801 agccggtggt tgccttgtat tccaccttcc tgcagcgggc ctacgatcag gtcgtccatg
    61861 atgtggccac ccagaatttg ccggtgacgt tctgcatcga ccgtggtggg ctcgtggccg
    61921 aagacggcac gacgcaccat ggcgcctttg atttcgcgtt cctccggcat gtgccgaaca
    61981 tggtcgtggc cgcaccgaag gacgagaacg aattgcagca catgatcaag acctgtgtga
    62041 gttttgatgg ccctgcttcc gtgcgctacg cacgtggcgt cagcctgggt gtgccgatgg
    62101 atcctgagcc aaccactctg ccgatcggca agggagagtt attgcgtgaa ggaacggatg
    62161 tggcgatcgt ggcgatcggc gtgacggtct ggccggccat gaaggctgcc gaacggttgg
    62221 cgcaggaagg catttccgct gccgtcgtca acgcccgctt cgccaaaccg ctggataccg
    62281 agctcatcct caaaacggcg aagaacgtcc gttgtctggt gacggtggaa gaaggttgta
    62341 agatgggcgg attcggttcc gcagtcttgg aaacattgtc agaagccgga gtcatgctgc
    62401 gcacgaagat ccttgggttg ccggattggt atatcgagca agggccgcag gacctgttgc
    62461 gtgagcggta tggtctcacc gccgacggca tttacaacag cgtcaagcag ttgttcggcg
    62521 ccggtgtcgt ggcggacgat gccgctcgcc tggcttcact cgtcggcagc ctgccgcacg
    62581 gtgatgagca aggcagctaa ggccgctgac acccatgcat atcaccagga aaaagacgcg
    62641 gcagattcaa gcgggatcgg tcaagatcgg cggtgatgcg ccgatttcag tccagtcgat
    62701 gtgctcgacc gatacgcgcg atgtgaaggc gaccgtggag cagatccggc aattggaaac
    62761 cgccgggtgt gagctcattc gcgtcgccgt tcccgacgac gaagcggcga acgcgctgcc
    62821 gaagatcaag gcggccatgt cggtgccgtt aattgccgac attcactttg atcatcgatt
    62881 ggcgctcaaa gcggccgaag tcgtcgattg cgtccgtatc aaccccggta acattggggc
    62941 ctggtggaaa gtgcaggacg tcatcaaggc cgtcaacgag cggggcattc ccctgcgcgt
    63001 gggcgtcaac ggcggctcgc tcgaacggcc gttgctagat aagtatggct ggccttcgcc
    63061 tgaagccctg tctgaatcgg ccttgaacgc cgtgcatgcg ctcgaagacg aaggcttcac
    63121 caacatgaag gtgtcgctca aggcttccga tgtgcaccac gcgatcgatg cctactatct
    63181 gttctctact caatccaact atccactgca tatcggtatt acggaagccg ggacggccat
    63241 gaccggtgcg gtgaagtccg cgatcggcct cgggtggctg ctttcgcaag gcatcggtga
    63301 cacgctacgc gtctcgcttg cggccgatcc agtggaagag gtcaaggtcg gcttcgaaat
    63361 cctgaagtcg ctggaattgc gtcatcgcgg gatcaatgtc atcgcctgtc ccacctgcgg
    63421 ccgtgtcgaa atcgatgtgg tgcgtatggc gaacgagttg gaaaaaaagc ttggacatat
    63481 caagaccccg ttgaacgtgt ctgtcctcgg ttgcgtggtc aatggcattg gcgaaggcaa
    63541 ggaagcggat atcggaattg ccggcggaga aggcaaaggc attctcttca agaagggaaa
    63601 gttgatgcga aaggtgccca tggatgaact catggacacc ctcattcacg aagtcgaaca
    63661 gctggctaaa gaaaaagagg ctgaagcttc cggtgtcacg cacaacgggt cgtcggacgg
    63721 acagaccgaa gaaggttggg aattgatggg ccatgcgccg gatgaacaat ccaccatcgt
    63781 tcgcgacatt ccagtgcttc ccagtaagcc gtaatccgcg gcattcgttc agcggctgtg
    63841 ttgtggaagt ttcctctgtt tctctgctct tcgcggttga gttcctgaac ctcccccttc
    63901 tataatccga cccgtggcgc cgtacccaag tggctaaggg agcagtctgc aaaactgcga
    63961 tgcggcggtt cgaacccgcc cggcgcctcc aaccattcct acagacatct cagagggttc
    64021 ggtggttcta accggcccct gttccacccg ctgagtttcg ttactcccgg ttttactccc
    64081 gagtccattc acactttttg ctgcaaaccc acattcgctc gattcatcgt tcttcgtaaa
    64141 tgagtcgggg aggagatggg cgtaatgcct cgtcatggcg ctcttggaat ggcccatgag
    64201 ttactgcacc gttcggatgt cagcatttga cactggatag tgtaaggcaa gagggctgtt
    64261 cggtcgggcg caaaagagaa aaacttgagg ctgtcgatgt cattgttggc gcgtgcgtga
    64321 gaagccaaag ccgtgccctt ttggatcaag aaccttacct cctgaattgt gaaatggtta
    64381 gaatacggaa ggcctgtttg atgctcagac caatttgcgg atgacgaatt tgttgtctga
    64441 aaggatgcat gacccctgat ccaatttttt ttcgagatct cgcctatgtg ttcatcgctg
    64501 cggtcgtcgg agggacttcc gcctatctgc ttcgccagcc attgattctg ggatatgtct
    64561 tcggtggcat tctcatcagt cccttgacgc ctggcccatc catctcagat gtccacagct
    64621 ttgaattgtt cgccgagatc ggcgtgattt tgttgatgtt cagcattggt atcgaatttt
    64681 cgttgaatga cttgttgcga gttcgttggg tcgccctcct gggaggacca ctcgggattt
    64741 tcctctccat tcttctctcc gtcgggctcg gccatctggt gggatggacc atgatccaaa
    64801 gcgtggtgat tgggtcggtg gtctccgtgg ccagtacgat ggtgttggcg cggttgcttc
    64861 tggatagcgg cgacttacgg gccaaacatg gcagggtcat gatcggaatc acattggtgg
    64921 aggatctggc cgtcgtggtc ctcaccgtgc tgcttccagc gctgggcacg ctggaaccgg
    64981 gtcgcttcgc taccattgga aaggcgttat ggttggcagt tctaattctg gttccgtttt
    65041 cctatctcgc ggccaaagcg atccctccgt tattgacgcg cattgcgaag acacaaaatg
    65101 ccgagctctt tctcctggtc tcgctatctc tgggcatcgg gacggcggcg gtaacacaga
    65161 tggcgggatt gtcccttgcc cttggcgcat ttctggctgg gttgatcata agcgggtcgg
    65221 agtacgggca cgaaacgctt gccaggctgc tctcacttcg cgatgcattc gtcgccctct
    65281 tttttgtgac gatcgggatt ctgatcgacc cgcgtatcgt gtggtccaat cttccgctgc
    65341 tcggcctcat gctcggattg attctcatcg gcaagttcgt catctggacc ctggttgtac
    65401 gacttttccg ttacgcctgg ggcaccgcac tcctcgtcgg cgtcggtctc acgcagatcg
    65461 gcgagttttc ttttgtgttg gtgcaggtcg ccaagacggc aggtcatgtg ggagaggacg
    65521 tgtataatgc caccctcgcc gcatcgttgt tgagcattct tctcaacgca ccgttcgttc
    65581 gatatgcacc gaggtgggtc gcggccttgc acttgcgaat gggacgggct gccatgccaa
    65641 tgcaaggggg cgtccaagac gagcaagcag ggcacgtggt ggtgtgcgga ttcggtcgca
    65701 tgggcagcac ggtgggcctg gcccttgacc attttgggat cgcctacacg gtgatcgagc
    65761 gcgacccgga cctcgtacga caattacggc gtcgcggcat ttcctgcgtg tatggcgatg
    65821 cctcgcaaac cgagctgttg actgcagccg gcgctgagcg ggctgctctg gtgattgttg
    65881 cgctcccggt gattcacgaa gcatcgctga ctgttcgacg gctgcgggga ctgaatgaaa
    65941 aaatccccgt gcttgtccgg gcgcatggat ttagggaggc agaagatttg agggcggtag
    66001 gcgcgacaga aatcatcctg ccagaaatcg aaggggcaca taccctgatt cggcatgcat
    66061 ttcaggcgtt gaaggtgccg aagcccgaca ttcttgccta tatcaagtcc gggcaacact
    66121 tccgcgcgtc cgatgccgac ggagaatagt gctgactgtt ctcacgatcg ttggcggacc
    66181 atcggatgga aagaagaggt cgtcctcgta agaacaggac gctggatccg gcaggggtta
    66241 ccgtccgaaa tggtaggcaa tgccgaacat aaacagattg gctcgatagt ctgccgtgac
    66301 attgctgcgg tcgaacttga attgagcaaa gttatacttg tactcaatga aaggggcaat
    66361 tgactcagtc atgtagtaac ggataccggc gaggacatta aagcttggag cgaaggatgc
    66421 ttcaggtcgg aagttgccgt tggaaatatt gcctacgttc aggccaatgc caagaccggc
    66481 atagggctcc actttgtact gatagccggt gactcggact agtgcattaa aggccaacga
    66541 atgcacctgc aaatcagcgc ccggcattgg ccccgacagt cgtggattgc tggaggtcgg
    66601 gagggtctgt tgcttgatgc cgggcgtgga ccggtacatg tctacttcaa ggccaaacca
    66661 attatagccc cggtcattaa agtagtgacc gattttgcct ccataaaaca gatccttctg
    66721 tagagagact ttgccggcat ccgtggtatc aaaactggga gcattcacgc cgatccccgc
    66781 agccacatag ttctcggcgc gtgtgacggc tggaatgcac aacagggcca gaaacagaag
    66841 accgattgac gaacggcgca tgcgccctcc tcttacaata gctgacggcc tcaggagcac
    66901 taaagtgcta ctctaacgag cttggcgcgg ggtggcaaga gagaagtgta tctcaagatt
    66961 gaccgcatga tgactcactt cggtgtagca gcgtggggcg ttgacagaaa ccgcgccagg
    67021 gcgtttgatc ccacgaccaa ttcaacagcg cgtactccgt atgggtgaga tgccgttcga
    67081 tcgttcggcc gaatccgccg agtcgtaaag aaaagccggc gggtaggatg aaatctttga
    67141 acaggccaaa ttctgtgaag tcacccgcat agtaggctgg gtcgccccgc tcggtgacaa
    67201 agtgttcccc gcgccagagc gtgatatagg gccgccaccc gtccaagttc acgcctgctg
    67261 tcagttgata cccgcgccca cgaacgattg gtgaactgct ctgcgtcggt tcgttctgac
    67321 tggtcagata cagggccatg acatcgattt cccgaaacca cgacaactgc ggccagtact
    67381 gcttaggctg ccaggtgaat tgaggaccga acgccgtgag gaaattgttg ccggcggggc
    67441 ggtcgcgggg cagtccaggg ccgaaaaacg tgcgggcttc tgaatattgg gcgccaccga
    67501 aatgggtcca atatacctgg ccattcagcg cgaagggccc tgctgctact cgacctgcat
    67561 agcccacgtc gaaccgctct gccttctgat acgtttcgat ctggttccag ttgatgaaga
    67621 gatcctgctg gtaatgttca cgatcaacca gcaattggaa gccttgttcc acggggcggc
    67681 tgaacagcgt ggcatcattg aataatgcgt ccacgaaagg atggttgcga tgcagggttc
    67741 cggccaccag tcgcacatcc ggcaccattt catattgtgc ggagagaatc ggttgcacgg
    67801 tgtgaaagga ctgtgtatct ccaacgggca atccggcgaa caggccggcg tcgagtgtga
    67861 gttgggggct gactcgatat tgaagtaccg gcgagagata ttgtcccgcg aacgtgtgat
    67921 ttgagaccgg gatgccgccg atctgccgta ccccgtgata ggagatactg taaacatagg
    67981 tcagtgtatc gagtttggct gtgaacctgc gctctgacgg agcgtcctgt cgatcatcaa
    68041 aaggccgaga cagcgaagcc catacatcac caatcaggtc ggcgtggttg gtatcccaat
    68101 cccaattgaa gacgagatat tcctgattca cccacttaaa cgtatccctg ttcatatagt
    68161 tgaggccgtc ttcggtgaag aaggctcgga tacggcgcgc ttgcgctccg aattcgatgg
    68221 aggcgcgctt gccgagacgg atcgttttcg agagcccgaa ttccgggaaa ttgctcgctc
    68281 taaattccgg gtctccctgt tggctgagga agttccggcc cttccaaaag gcgaggtagg
    68341 gacgccatcc ggccaagtcg agccaggtgc gtaattcata accccgcccg cgcgacgcgt
    68401 ctgagccatt gtcggcctgg ttgtagctag tcaggtaggt gaaactgata ccaatctgat
    68461 gccaccaagc agtagatgcg aaccatcggg atggttcaag caccaattct ggacccgtgg
    68521 cgacgacgag attgtcgcgt gtcgaaaacg atcggtctag cttgaacagt gcctgcccgt
    68581 tgcgcaccca atgggcctga ccattgaaat gcaaagggcc gaacttcagt tgcccggcat
    68641 acccgacatc gtatcgattg ggggccgacc cacgaaaggc ctgctcccag ttgatgaaca
    68701 ggtcttgctg atagtgggtg gaatccacta gcatttgagc cccctgttca atcgggcgca
    68761 ggaaccggtt gccgttgtca aacactgcgt cgtggaagtc ccggtgcggg gtgcgaatcg
    68821 tgccggcgac tgctgtgatt tgctcgaccg gttgataggt caggcggacg acgggacgga
    68881 cctgtgagat ttcgtgatcg tgtccaaagg gcatgttggc aaagacgccg atttcggcct
    68941 ctagcgaggg aagtagacgt ttgtagagcg tcgcgttgat caggttcccg atcaacgtcg
    69001 tgtcgcgtcc caggccgttg gtgaaggtat tatcctccag tccccgtttt tcaagattga
    69061 gtagaaacgt cgtcgtggag aaccgcatgt cgacgacgtg ggaggtggtg ggggaggcag
    69121 attcctcagc tcgaagcacg tcgtggacat tcaggtagag cagcgcgaga agcagccctg
    69181 accatagtgg gctgatggga aagcgatggg agagcagtgg tttcacgcgt gggggccgat
    69241 gataagaatt tggcaggcgt ggcgtcaatc a
//
LOCUS       DAOQEW010000003        64043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_72_length_64043_cov_9.013768, whole genome shotgun sequence.
ACCESSION   DAOQEW010000003 DAOQEW010000000
VERSION     DAOQEW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 64043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64043
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_64043_cov_9.013768"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..759
                     /locus_tag="PLS35_00605"
     CDS             16..759
                     /locus_tag="PLS35_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI17171.1"
                     /translation="MKYRGASQLMLGGMAMVLLTAPGCSGMKWFQSGSDESSNSGSSL
                     SASSGASGGYGRGEAGRDGQYPSLGRQDGSSQQNDASDTEGGKLRGFSPLVNGQIAGE
                     ERLSRSPIGSMLTSNEQDAKWAAEIRREEAAALEAGLQDVFYGYDRYNVSDNDGVASL
                     TVNAGWLKENPKALLKISGHCDERGTHDYNLVLGEKRAKAARSFLIDMGVSAKQVAIV
                     SYGKDRPFCADHDEVCYQQNRRGHMLLRK"
     gene            1076..1240
                     /locus_tag="PLS35_00610"
     CDS             1076..1240
                     /locus_tag="PLS35_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17172.1"
                     /translation="MNQRFGITVKFLISADSPEEAEEIIESACEKMASSIHEGDVSYE
                     GIEDIEEEDE"
     gene            1364..2755
                     /locus_tag="PLS35_00615"
     CDS             1364..2755
                     /locus_tag="PLS35_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HNI17173.1"
                     /translation="MTDTVESTGTHSRPPGFLNRIHQSNKQYLPQWLGVPAHIHHVAY
                     RMANPPVERPNSRREFQHLLQALEISEGAIEERFGYGFKTAPNGDRLVVVWEAHTEYY
                     SYQVWHVVGDSNTPLDFGPITFPGYQMPLCPLGLRVNALDLLFLPQELPLDRELPARL
                     PGAMVYGSRILGDDIVAVTSFTPDEFDRERYLICSTSEQALRQQVAKIVDTIVAIENY
                     YHLVMLPMKAFSRAVDQIHDYEQRHLYQRAVLIEQLGGTNPPTMQKWLTVLTQDLLQV
                     SRLAESMRYRLSASVPYDRIVQSNLVALQERPYPPLRQISEYVSWKITGVTDGYQQLL
                     RRIDAMEKDFEATVAVLRTHIELRLQEQNIQLQDQNMKLLASVDSTTRAQAILQRTVE
                     SLSVIVITYYLTGLGSYVLKACYEMGWVKNANVATAIFVPIAFAISFTLMTVGRKIII
                     KRMANPAGDPGGH"
     gene            complement(2847..3209)
                     /locus_tag="PLS35_00620"
     CDS             complement(2847..3209)
                     /locus_tag="PLS35_00620"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17174.1"
                     /translation="MDTDLRSSTRVTVTYPVRLSGDSMIGQGTVVNLSAPGCAIETTL
                     PVRPGDYLELHVMAPDQARPLNVGLAKVRWATEQKAGVEFIRVRRDEQSRLQRLIGQV
                     LEESAIGSERDRQELTAA"
     gene            3559..3879
                     /locus_tag="PLS35_00625"
     CDS             3559..3879
                     /locus_tag="PLS35_00625"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17175.1"
                     /translation="MERRDTPRYEIKVPLTFSGHAIDGSGLVTSLSKDGCHVTSEESL
                     PMRANLSLHVQFPAPYEPLTVEVAEVRWTNATGFGLVFVHVHDEEHARLQRFIDWLKR
                     TQNN"
     gene            4230..5741
                     /gene="zwf"
                     /locus_tag="PLS35_00630"
     CDS             4230..5741
                     /gene="zwf"
                     /locus_tag="PLS35_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNI17176.1"
                     /translation="MDLAQLRTPCVMIIFGIDGDLAKRKLLPALYNLMAGHFLPEQFA
                     IVGLDRSETTTEEFRAKLDRLMPEYLPENADRAVWQAVSQRVHYMAGDFQEGATYRRL
                     NDLLQGVDASAGTQGNYIFYMATIPGLFGRIVTHLGEYGLTVERDGTWRRVIIEKPFG
                     HDLDSAKALNHELQRVLQERQIYRIDHYLGKETVQNILVFRFANGIFEPLWNRRYIDH
                     VQITVAESLGVEHRGRYYEQAGAMRDMVPNHLLQLLCFLAMEPPNSFAADAVRDEKAK
                     VLRGVVPLSSLDVLRFVAFGQYGEGAVDGKPVVGYRAEPHVGAESTTETFVAMKLFID
                     NWRWAGVPFYLRTGKRMTRRLTEIVVQFKQAPFMLFRKTQVDRLAPNVLVIRIQPDEG
                     ICLRFDAKVPGPTVRIGEVNMDFQYADYFGNAPQTGYETLLHDAMAGDATLFQRADNI
                     ELGWSVVQMILDVWKSLPGSAAFPNYQSGTWGPPEAEALLRREGREWWNGGQA"
     gene            5774..6319
                     /gene="msrA"
                     /locus_tag="PLS35_00635"
     CDS             5774..6319
                     /gene="msrA"
                     /locus_tag="PLS35_00635"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNI17177.1"
                     /translation="MAQAGMEVATLAGGCFWCLEAVYDQVKGVQSVESGYIGGQIDEP
                     TYEQVCGGRTGHAEAVRVTFDPAVVSFRDLLEIFFVIHDPTTRNRQGNDVGTQYRSAI
                     FYHTPEQQQAAQEMIAAVTQEHLYPNPVVTEVVPATRWFEAESYHQEYFARNPLEGYC
                     TYVVGPKVAKFRQRFLALRKP"
     gene            complement(6334..6771)
                     /locus_tag="PLS35_00640"
     CDS             complement(6334..6771)
                     /locus_tag="PLS35_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17178.1"
                     /translation="MNPVQQSPRQGAEPPLWSIAAAAAVILLTPMLLFSLAPDGPIRD
                     GDTVFSTGAHKVPLFKPEQHRQAGYDATCLLDPKDPMIVLHTPAEGSDEEDIIAQVQG
                     KNTIEWPFCPPQAELIIKPYHVTQQPSMIKDLRDGFLRLFKSS"
     gene            complement(7476..8351)
                     /gene="ttcA"
                     /locus_tag="PLS35_00645"
     CDS             complement(7476..8351)
                     /gene="ttcA"
                     /locus_tag="PLS35_00645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNI17179.1"
                     /translation="MQPTSTKPVPAPARPAAQSEEIERVETRLCRLAGQAIADYRMIE
                     DGDKVMVCLSGGKDSYGLLEILLSLRSRAPIHFDIIAVNLDQKQPGFPAHVLPEYLTR
                     RGVPFHIETRDTYSVVKRLIPEGQTTCSLCSRLRRGHLYRLSTELGATKIALGHHRDD
                     ILETLLLNLFFTGKMKAMPPKLRSKSGRHVVIRPLAYVKEADLARYAALLQFPIIPCD
                     LCGSQEDLKRKQVKQMLQDWNQRFPGSNDSMFAALGNIAPSLLLDRSLFNFSDLKADA
                     ASPDASVSDAEEDLL"
     gene            8630..9691
                     /locus_tag="PLS35_00650"
     CDS             8630..9691
                     /locus_tag="PLS35_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HNI17180.1"
                     /translation="MGRTQIHRRQRSEPGGRVPVWAEWTDEQLLDLRMCDLDLRLDGT
                     FYQEPIEQLYRELDARRLVFRPHFWLSDEWFTPDGVPGIAVPFYLAHPRLAKLEASQM
                     LEVEGGTHDWCMRILRHEAGHAIENAFLLRRRRRRQKLFGRSSQPYPEYYTPRPYSRS
                     FVRHLDVWYAQSHPDEDFAETFAVWLDAGSLWKERYRGWPVLKKLDFMERLMGELAGV
                     APMVTGRQQLDPLPRIYKTLRDHYEEKRKHYGIGRASSYDTDLNKLFSAGVAYAKNPS
                     AAEFLRRTRKEIRRRVAEWTGEYQYTIDQVLEGMIERCHASKLHLTQQTEQAKLDFAI
                     LLTVQTMNYLHSGRHKVAL"
     gene            9688..10710
                     /locus_tag="PLS35_00655"
     CDS             9688..10710
                     /locus_tag="PLS35_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNI17181.1"
                     /translation="MRRMRVLVLMHKDLVPPEQLNGQDLDTAAWKTEYDVVSTLRKLG
                     HDVQALGVKSDLEVIRAAVEDWKPHIAFNLLEEFDGVAVYDQNVVSYLELMRIPYTGC
                     NPRGLMLARDKALAKQVMSYHRIPYPEFMVVPLHRMVRRPKYLPFPLIVKSITEEASL
                     GISQASIVDDDAKLRERVAFIHDNVGTGALVERYIEGRELYVGVMGNAHLQVFPVWEL
                     VMDKMPDESRRIATQRVKWSRKYQDKYGIRSCEARNLPDGVIDHIQHLAKRVYRALGL
                     SGYARIDMRMDQAGTVYVIEANPNPQIASGEDFADSAEKANLAYKDLLQELLHVGLRW
                     RPAQAA"
     assembly_gap    10966..11065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11066..11547)
                     /locus_tag="PLS35_00660"
     CDS             complement(<11066..11547)
                     /locus_tag="PLS35_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17182.1"
                     /translation="MTYARRIRLFLAGLAFIGFLSPCAAQSADPQEPHWGFATDLGLW
                     TGTTNNTTFALGFGLDYYMDPNFSFGGMALFTPVGDLTQIGVAGVAKYHLRLNSGFNL
                     VPFAGLGILHADLNRGSGPGKIDRNDTSHFIPLGMSLEYQVGPKIAFSTTLMVNLHHI
                     T"
     gene            11688..12182
                     /locus_tag="PLS35_00665"
     CDS             11688..12182
                     /locus_tag="PLS35_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17183.1"
                     /translation="MKKESMTPAEAADALYKLMPRRITVEMLSDYGIQGEDAHEDAMT
                     RELLSFTLYWVLAAINAHIPRKYRDLVFHRVMDLIRADWETEYKLGALKWEEYLDEVE
                     ARRARYAPVGDYEGGAIAASEEISDLLETEGLIQPEDRPKLLVLLPDLVPLDKYQELL
                     SQCV"
     gene            complement(12229..12834)
                     /locus_tag="PLS35_00670"
     CDS             complement(12229..12834)
                     /locus_tag="PLS35_00670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNI17184.1"
                     /translation="MSKLVLIRHGESQWNLENRFTGWVDVPLSPKGIEEAKAAGKKLA
                     GFTFDRAFSSVLARANETLRFILEAIGQTTIPVEKDKALNERMYGELQGLNKAETAKK
                     FGDEQVKIWRRSYDVRPPGGESLKDTAERVLPYYESRIKPYVLKGETILIAAHGNSLR
                     ALVMQLEQLTREQVLELNIPTGAPLLYELDTNGKVQSHRYL"
     gene            13025..13858
                     /locus_tag="PLS35_00675"
     CDS             13025..13858
                     /locus_tag="PLS35_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17185.1"
                     /translation="MPDVSPSDWSSLQQWWQEMGRRLQVEDRVEAFFRRAMGASQALA
                     GAVQVGGEVEYILFVLVRYWIPVVLPPPGRERASKGDPDYWLESEQILKAAAVRLREL
                     KPLIELLTASNPLSAGVAEPQAARPPMVEVELANMLQGIAESAGSYGGPDYTSVIKTF
                     DPVPLRQTQPFKHNKKNSAELWVVFLLREHFRSLGLGKDRYWPLVAGLCAAAGIANPN
                     GQPYGPDELKSWWQKNWPRTYTQLEQTQAAPDLSGAAYQQDFDWFVSWIEWQRQQISE
                     V"
     gene            complement(13882..14523)
                     /locus_tag="PLS35_00680"
     CDS             complement(13882..14523)
                     /locus_tag="PLS35_00680"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNI17186.1"
                     /translation="MKTCSQCRQENRDDARFCHQCGAPFALEARAAASETEQDAPQTD
                     TELLKAFIGPNADRYLETFKKFSGPGGPQFALSWHWPAFVFEPFLWFLYRKMYVYALI
                     YAIGPAMAFYITQDLSADIVWRVIAGGSANYIYFWHVKEQLAKIKSERATGGETREQL
                     LGELGGVQPYVVWVGVGLLVLKIGLVVAMFKEGPPDGPKGPPAKPHPASLTHV"
     gene            14692..15897
                     /locus_tag="PLS35_00685"
     CDS             14692..15897
                     /locus_tag="PLS35_00685"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNI17187.1"
                     /translation="MPTGELRLNAEQYLMNTYQRQPISIVRGRGSKVYDLEGREYIDF
                     VAGIAVNVLGHGHPDLVLAIQKQAQHLLHTSNLYYTEPQVRLAQTLVEHSFAQKVFFC
                     NSGAEANEAAIKLARKYSYDKYGADRHEIITMTSSFHGRTMATLTATGQDKVQKGFAP
                     LMPGFSYVPFNDLSALERAITPKTAAIMLEPIQAEGGVHVAERGYLQGLRELCRERDV
                     LLIFDEVQTGIGRTGTLFCYEQFGMQPDIMTLAKGLGGGVPIGACLATDSVARAFGPG
                     THASTFGGNPLACASALAVLRVLLDGKILDQGRRMGEYLAKGLATLKERHRCIKEVRG
                     MGLLQGMELDVDGKTVVADCLTRGLLINCVGERVLRFVPALIITEREIDRLLASLSQI
                     LSQRTTSTH"
     gene            15914..16894
                     /gene="argF"
                     /locus_tag="PLS35_00690"
     CDS             15914..16894
                     /gene="argF"
                     /locus_tag="PLS35_00690"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNI17188.1"
                     /translation="MLRRLRRSTPRTGLGKDFLDLLSIPTDELTGLLRLAAQLKVKQR
                     RGVSHPLLQGRMLGLLFQKPSTRTRVSFEAGMNQLGGQAMVLPMGDIQLSRGESIADT
                     AHVLSRYLDAIVLRTFDHAIAEEWAREASIPVINGLTDLNHPCQALSDLLTIQEKKRR
                     LKGLKLAYVGDGNNVTNSLIEAAAKMGMTIAVGCPPGYQPDRRIVELSLAEAERTGAV
                     IEIGSDPSVAVKDADVVYTDVWISMGQEREQAKRLKTLAPYQLNARLLKCAKPDALVM
                     HCLPAHRGEEISAEVLDGPQSVVFDQAENRLHMQKAILVRLLGKKTARKT"
     gene            16909..18126
                     /locus_tag="PLS35_00695"
     CDS             16909..18126
                     /locus_tag="PLS35_00695"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNI17189.1"
                     /translation="MSQSSYKKVVLAYSGGLDTSVILKWLEEVYGCEVVAFCADLGQG
                     EDLKAIKKKAQSLGVKKVYVEDLRETFVKDHVFPMLRGNAIYEGSYLLGTSIARPLIA
                     KRQIEIAAKEGAAAVCHGATGKGNDQVRFELTYMALHPQIKIIAPWREWTMRSRRELI
                     EYAEKHGIPVTATKAKPYSMDMNLFHTSYEGGILEDPWEAPPEEIFVMSVSPERAPAK
                     AREVEIEYVAGNPVAVDGKKMSPAALLGHLNKLGGEHGVGRVDLVENRYVGMKSRGVY
                     ETPGGTILHAAHRGLESLTMDREVLHLRDSLIPRYAELIYYGYWYAPEREMLQVALDE
                     AQKDVTGTVRVKLYKGTCTVVGRKSPRSLYRLDMATFEEDDVYRQKDAEGFIRLNALR
                     LAIRAQRKKRPGR"
     gene            18126..>18610
                     /locus_tag="PLS35_00700"
     CDS             18126..>18610
                     /locus_tag="PLS35_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNI17190.1"
                     /translation="MGKSTPVGARGRKSARAPQRSGRALPKGKAWGGRFAEQTDRLVE
                     QFSSSIAYDRRLYPYDIQGSIAHCKTLERAGVLTGRESGQLVRGLQFVKVELDGGRFP
                     FSPQDEDIHMAIERRLTELIGPVGGKLHTGRSRNDQVALDLRLFLRDVLSTFMKQVQE
                     FR"
     assembly_gap    18611..18710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18711..19370
                     /gene="argH"
                     /locus_tag="PLS35_00705"
     CDS             <18711..19370
                     /gene="argH"
                     /locus_tag="PLS35_00705"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNI17191.1"
                     /translation="STLSLIMMHLSRLSEELILWASQEFRYVDLPDTFCTGSSMMPQK
                     KNPDVPELVRGKTGRVYGHLMGTLTLLKGLPLSYNRDLQEDKEALFDAVDTTGQSLVL
                     CTELVRRLAVNRDVMAEAAEGGGMLATELADYLVTTGVPFREAHSITGQIVRFSLEKR
                     RPLQRLTLKELQGFSSHFKADVLNCLTVRGAIDRKAQIGGTARRRVEARIKELEKALK
                     G"
     gene            19367..19669
                     /locus_tag="PLS35_00710"
     CDS             19367..19669
                     /locus_tag="PLS35_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17192.1"
                     /translation="MMSAGARRAAIQPHALLATVSFILLAGCGVLGAPIPPEDVGVAP
                     VVERQLRREGLLASGANVRGSASRPSAPSGLTIEETPIPPDPDPLPVSPWRTMGTR"
     gene            19769..21031
                     /gene="lysA"
                     /locus_tag="PLS35_00715"
     CDS             19769..21031
                     /gene="lysA"
                     /locus_tag="PLS35_00715"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNI17193.1"
                     /translation="MHDFQYRNGELYCEEVPLSRIAKEVGTPCYVYSHHTLVRHFRVY
                     DGAFKNIPHIVAFAMKANSNLAVLRLMAKEGSGVDIVSGGELFRALKAGVPASKIVFA
                     GVGKKQEEIRDALKADILMFNVESSAELQAINEVAASMDVKARVALRINPDIDPKTHP
                     YISTGLKKSKFGIAADRAIEEFKNAAAFSHIKVVGLHAHIGSQLTQVTPFVESLKKVL
                     TMVQTLAEQGIPLRYVNIGGGLGITYSDETPPEPKDLAEAISPLVRDLKCVLIMEPGR
                     VIVGNAGVMLTKVLYAKDGEAKRFLIVDAAMNDLIRPSLYDAYHDIRPVYEKVTQGEK
                     KTVDVVGPVCESGDFLAKDRVMPAMGAGELMAVMSAGAYGFVMSSNYNSRPRVPEVLV
                     HEGQYHVIRSRETYEDLVRGEHVPEFLS"
     gene            21068..21940
                     /gene="dapA"
                     /locus_tag="PLS35_00720"
     CDS             21068..21940
                     /gene="dapA"
                     /locus_tag="PLS35_00720"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNI17194.1"
                     /translation="MFTGSLVAIVTPFKNGKLDERALGDLIEWQINSGTHGIVPCGTT
                     GESATLTHAEHDRVVAFTVEVVRRRVPVVAGTGSNSTEEAIALTKHAKAAGVDGALLI
                     TPYYNKPTQEGLFLHYKAVAEAVELPLVVYNIPGRTGVNMMPSTIARLTVCPTVVAIK
                     EGSGAVQQASEIIQLCGERLTVLAGDDALTLPMMAVGGKGVITVTANLVPTKMAELVN
                     AFRDGRLEAARAMHYELYPLFTALFYETNPIPVKEALHLMGKIAPEIRLPLCPMGNDN
                     KAKLLTVLKAAGLV"
     gene            21970..22773
                     /gene="dapB"
                     /locus_tag="PLS35_00725"
     CDS             21970..22773
                     /gene="dapB"
                     /locus_tag="PLS35_00725"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNI17195.1"
                     /translation="MIKVVITGAAGRMGSRLVSLVKDSAFLTLAGAVESKGHHALGED
                     SGEVAGCGRTGVPIVEDLSSLMERGEVVVDFTTPAATLGHLRIVAQHRRGIVVGTTGF
                     SPSELDELRSVSKQIPCVFSPNMSVGVNVIYKVIAEMAKTLGEDYDIEVIEAHHRLKK
                     DAPSGTALKMAEVLARAVNRDLGQVGVYARKGLIGERKKGEIGIQTIRAGDIVGDHTV
                     MFGTMGERIELTHRASSRDTFARGALRAARWLVKQPPGLYDMLDVLSLK"
     gene            23452..23712
                     /locus_tag="PLS35_00730"
     CDS             23452..23712
                     /locus_tag="PLS35_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNI17196.1"
                     /translation="MYRFFLIAVLLIVLYYLIRRAIRKIGQDGALEKPKDGRLSGNQM
                     VQDPVCRVFVARENAVKENIGGQTYFFCSRGCAAAFEKQLSG"
     gene            complement(23709..24011)
                     /locus_tag="PLS35_00735"
     CDS             complement(23709..24011)
                     /locus_tag="PLS35_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17197.1"
                     /translation="MRASVWLLAVGLLVGACSHNPYLDNSLTRYQGKDRAWIEKELGP
                     PDAKTTRFFGGEKWTYNRIAGGKAGPPLFNFKANECQMILFFDKDDTVSDSTYSGC"
     gene            24184..24831
                     /gene="fsa"
                     /locus_tag="PLS35_00740"
     CDS             24184..24831
                     /gene="fsa"
                     /locus_tag="PLS35_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNI17198.1"
                     /translation="MKLYLDTANVKEIQEGANLGLIDGVTTNPSLVAKEGRSFKEMLL
                     EICNMVDGPISAEVVGVESEAMIKEGRDLAKVHKNIVVKVPLIPEGLRATKKLSAEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDVSSDGMALIRQIVTIYKNYDFKTQV
                     LVASVRHPQHVVEAALAGGHICTMPYSVFQQLVKHPLTDIGLKKFLADWDAMGKK"
     gene            complement(24838..25545)
                     /locus_tag="PLS35_00745"
     CDS             complement(24838..25545)
                     /locus_tag="PLS35_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNI17199.1"
                     /translation="MSTLSMLNADIIACTQCPRLVSYRESVAATKRRQYREWLYWGKP
                     IPGFGDPRARLYVLGLAPAAHGGNRTGRVFTGDRSGDWLYEALHRFGFANQATSTDVD
                     DGLALTDCYIGATVRCAPPANKPTPDEFTACRPFVLGELRLLEHVQVVVVLGKIAFDH
                     YFKACREMGRELPSPLPKFGHGVAYELAWGVSLIGSYHPSQQNTFTGKLTRPMFHRIF
                     SDAASRLEGNRRGSPSG"
     gene            25640..26716
                     /locus_tag="PLS35_00750"
     CDS             25640..26716
                     /locus_tag="PLS35_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNI17200.1"
                     /translation="MRNNDWGLRAFVVGVTMVLGLAPLFAEVSLDSKAPAPSSPSASD
                     DSRVPIPERGTGPLSAEPEGAPELEDRLVILPEIKREGERFFLSSFKLPDKLTFAGQS
                     IPLDNWQVRERIEYEFYQFLEDQGESIILAKRTGRCFAPAEKQLADAGLPDDLKYMLL
                     VESKCIAAAYSRAKASGPWQFINSTGKRYKLHNERWLDERRNLEMSTEAAIKYLRYLR
                     ELQGDWFLAMASYNAGEDRVRKLLKEQNVTDFWRMHGPRETMRYVARIIAAKEIYSQP
                     EKYLGLTKKDLYPPLETETVTVTIKEAQRRLTSIAEEYGTYFLELKMLNPEFTKDYLP
                     KGSYQVKVPRQTCPNRCFKQDKLP"
     gene            26722..28062
                     /locus_tag="PLS35_00755"
     CDS             26722..28062
                     /locus_tag="PLS35_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNI17201.1"
                     /translation="MIEIGVPQPRTRALLARLLQAGLHAVDPYEAVRRQVSIGRRHLT
                     IGSQQYSLNQVKRIVVVGAGKASARMAQALEHQLGPRIDAGLVVVKYGHGAPTNTIRI
                     VEAGHPIPDVAGLEAGRQVMAVVKTLTRDDLLIVLLSGGASSLLPAPVSGITLQDKQQ
                     TTKLLLRSGATIHEINAVRKHLSSVKGGQLAAATAARVASVILSDVIGNDLGTIGSGP
                     TAPDPTTFREAWEIVERYGVVKTIPASVRNRLSMGMRHRAPETPKPRASVFRRVDNIL
                     IGDNQAAVDAVATAAQQQGLRTLVLSTTLTGEARELAKMFGAMAREMARHGRPIPRPC
                     CIIAGGEPTVTIRGEGTGGRAQEFALAAAPEIAGLPNVWVAGFATDGTDGPTSVAGAV
                     VDGQTVVRARRKGLDLSQALQDNDAYPFFEKLHGHIVTGPTGTNVNDLYLLLAL"
     gene            28078..30708
                     /locus_tag="PLS35_00760"
     CDS             28078..30708
                     /locus_tag="PLS35_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNI17202.1"
                     /translation="MIPSLILSLEESRDPSLVGGKAAGLNRLLAAGFAVPRGLCVPTT
                     VYRHVLAAGGINAEEVWRRVLQASQEERSQELARVHACLLKQAWPTGFQLALERRLVE
                     LTHDPTTRWAVRSSATNEDVEQASAAGLYSTSLGLSSSEILRGICDCWQSLWSDAAVQ
                     YALRTSSSKSFPSMAVVIQPLLQATTAGVAYSIHPVTGRTSQVVVNAVPGLAASLVSG
                     EVTPDQYVVEVAEDEGRPIRIRRRLLARKVQKLLSTPAGLHTESISEAGQKQSSLSDA
                     QLFELATLSKHIEAAFGHPIDLEWVWDADKVWVVQARPITAVQPSPALTNDECEWTRA
                     NLKETMPELPSPLGLSFLERFMDAYIVGPYRRLGCTVPEGLSSVRVMHGRPYLNVTLF
                     YTLVTQLHGNPSFLTEQMGGEPLGFTPSVRPLSGLALVRAGVLMMREWRRVVRQGPAN
                     FVAMKDMAQTYRPDRIRNLSAKELAATLDRLGQWLDTHEVTFGIAGGVAQSLQAMGTF
                     LPGWLGEDWRELLNASLQGQGSVISAQHILRLAELVEVASRESVVQRWFLREGWAASG
                     FREALAGTGFLRRFDEYLADYGHRAVGESDIMSPRIADQPDSVLMLLRTQLREGKAVP
                     PGDVMTQQAQRREAALAEIYRRFGWRRHRGVVFRWWYRRLSRFCALREANRHHLLYYS
                     TAVRHLLLQLGERMVEQGILTQPDDMFYLTMDERVALNEGASGDWQRLVRTRREQRTR
                     DQNASVPDTVRDWEAVLEQGPSMTGQVDGGLLRGIAVSAGTTSGPVRIVRSTQDWQRV
                     RPGDILVVPVIDPGMAPLFGIAAGLIAEMGGTLSHGAIIAREYGLPVLVNVPYATTLL
                     REDEPVHIVSSTGTISRLVP"
     gene            30804..32213
                     /gene="rmuC"
                     /locus_tag="PLS35_00765"
     CDS             30804..32213
                     /gene="rmuC"
                     /locus_tag="PLS35_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNI17203.1"
                     /translation="MIDLTSTTFIAGGVAGLGIGVLVTGWWVTARTQSRIQAQLLESG
                     ERAQRADALAVALQQRLDTQQSEVDQLRQALAESQEGRTKAETRMEVISRSMEDQKAM
                     LSQARQELQESFEALSGQALKQNNAAFLNLARASFETLHAEAKGELSQRHQAIGELVK
                     PLEESLHRYQEQLQQAEQIRQREYGGLDQQLKFMAESHQRLQQETSNLVKALRAPAVR
                     GRWGEMTLRRVAELAGMVAHCDFLEQDSVDSMDGLLRPDMVVYLPAGRQIVVDAKTVL
                     AAYLDAYEAQDDQQRQAHLRRHANQVRTRMDALSLKAYWNQFGQTPEFVVLFLPGEQF
                     LGAALEQDPSLIEDGFAQGIVLATPTTLMALLRAVAYGWRQEQLTEHAEQAGRLGKDL
                     YERMAVLTDHLNDIGQALGKSVGAYNKAVASLETRVLPAARKFKDLGISSEKDLAMLE
                     STGIEPRTGFPFAAEDMRT"
     gene            32216..32644
                     /locus_tag="PLS35_00770"
     CDS             32216..32644
                     /locus_tag="PLS35_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNI17204.1"
                     /translation="MMQPQDMVAEFHRKFDIPIGERPSIPPDATRQLRVRLIQEEFAE
                     LQEAMTAQDLPAVAKELADLLYVVYGTAVSYGVDMDPVFREVHRSNLSKVGGYKREDG
                     KWVKPPTYSPADVKPLLAKQLSGSVGSASHLETRSQESEA"
     gene            32646..33155
                     /locus_tag="PLS35_00775"
     CDS             32646..33155
                     /locus_tag="PLS35_00775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17205.1"
                     /translation="MKELYCPACGTPCVRVTSGTSLIEKTLNRLSIFQVRCQLCTTRF
                     QARRPGNRLTTQAFDRREYRRLPVNFAGTLRLDHTAAGGVVTDISMGGCTLQATTPLP
                     RGTFVTFVLHGPSGQPDIKVEAAMICSIQPQSIGMKFLEFQPEDKQRMGQLVLELLAY
                     QQAPPRISV"
     gene            complement(33237..34127)
                     /gene="panC"
                     /locus_tag="PLS35_00780"
     CDS             complement(33237..34127)
                     /gene="panC"
                     /locus_tag="PLS35_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNI17206.1"
                     /translation="MQSTPCAESSHVKIIRTTSAMANWSRGLHREGVTIGFVPTMGAL
                     HEGHRALVRAARLACDAVVVSIFVNPTQFGPTEDLAKYPRQFRLDRALCAKEGVDAIF
                     APTATAMYPAGFQSIVAVPSLARRWEGEARPHHFQGVATVVTKLLSLVQPDISWFGQK
                     DFQQAALVRQLAKDLNLSGTLRVHPTIREHDGLALSSRNVYLSASQRDAAPVLYRALQ
                     AGAAAIRSGERRGALIQRLMTQMIKTERSVTPEYLAVCDPSTLEPLPTIQRHAILLGA
                     IRLGTVRLLDNMPVAIRRKP"
     gene            complement(34091..34654)
                     /gene="folK"
                     /locus_tag="PLS35_00785"
     CDS             complement(34091..34654)
                     /gene="folK"
                     /locus_tag="PLS35_00785"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNI17207.1"
                     /translation="MATAFIGFGSNLGNRIDFCDRAVTLLGLLPHSRLDAVSSLYETE
                     PIEDGAAPGPAWFLNGAVQVETNITPKSLLEICREIERALGRDQDNRKGPRTLDLDLL
                     LYDDCVLNDPGLIVPHPRLHQRRFVLAPLVELDPDRRHPVLGQTLRELLETLADPSIV
                     RRLDPQPNSRYGSRPACSPPPVQSPHT"
     gene            complement(34746..35921)
                     /locus_tag="PLS35_00790"
     CDS             complement(34746..35921)
                     /locus_tag="PLS35_00790"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNI17208.1"
                     /translation="MAGFPIEVATRIKTLPPYLFAAIDKMKQEALARGVDIINLGIGD
                     PDLPTPAPIIESLYEAAKNPKHHQYPSYEGMLSFRKAVADWYKRRFNVTLDPANEVLT
                     LIGSKEGIGHVHLAFIDPGDIVLVPSPGYPVYPVGTSFCGGVSHIMPLTKANGFLPDL
                     NAIPKDVAKQAKLMWLNSPNNPTSVIMSKDYFKRVVEFAQENQIIVCHDAAYSEIFYD
                     GKRPASFLEVDGAKEVGVEFHSLSKTYNMTGWRIGFAVGNKEVLAGLGRVKSQLDSGV
                     FEAVQAAGITALGLDDSVTDGLRKIYQERRDTLVPGLKKLGLEVDAPPAAFYIWVTVP
                     KGYTSASFTAHLLEKAGIVTTPGNGFGAPGEGYIRMTVCTTKERLAEAVERIRKVGF"
     gene            36183..37385
                     /locus_tag="PLS35_00795"
     CDS             36183..37385
                     /locus_tag="PLS35_00795"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNI17209.1"
                     /translation="MDATSLWPAAGVVLVALATAMFLPRTTLQEASSTPRYPSLDGLR
                     GYLALAVFVSHSSIWYFYLRSGTWDVPPSNVYTQLGQGSVTLFFMITGFLFWSKLLDG
                     RHQPIDWSRLYLSRALRLGPLYLVAVACVLLMSFGRTGFQMREAPAVVAEQTAQWLLF
                     TIPGISSVNGFAETVPLAGAVWSLPYEWLFYAGLPLAAFCLRTSPPIQWVIVSLAAVV
                     ILVMVMPQVRFPILYAFAGGIAAAGLVRIAAIRMMLSRGVWGAIAILCLATAFMAFPT
                     AYTVPATLLLAGGFLIIACGNSLYGILEWPASIVLGEMAYSLYLLHSVVLFVTYRLLL
                     AESAATLSPAEHWAMVLCQVPALILLCSATFRLVEKPGMEAVPRWHARLVARRADTSA
                     SAVPSGRR"
     gene            37475..37831
                     /locus_tag="PLS35_00800"
     CDS             37475..37831
                     /locus_tag="PLS35_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HNI17210.1"
                     /translation="MKILAPCPSSPNCVSTQAADQSRQIAPYRYQQNLAGAQNTLKTI
                     INRLPRTRLVEENDGYLHYEFTSFLLRFIDDVEFVFDDATKMIQFRSASRTGYSDLGV
                     NRRRMEEIRTLVEGKL"
     gene            38001..38528
                     /locus_tag="PLS35_00805"
     CDS             38001..38528
                     /locus_tag="PLS35_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17211.1"
                     /translation="MIRRAIHLTAVLSLALSSACVSVQVDPLTTKAFEPRSGADAVTT
                     LQREPNHGHVQIARIVATSEYASEDTLRDRILERAKELGADAVVLGDADVRRLSDRGP
                     TFQSTMGTAVPGGVSNTNSYRSGYWNPFRMDAWSFTQGAGGGQGWMLHMSGLAIRYLS
                     AEEVRAAQKPPLSTP"
     gene            38875..39732
                     /locus_tag="PLS35_00810"
     CDS             38875..39732
                     /locus_tag="PLS35_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17212.1"
                     /translation="MSVVNIDQRHPAVLPHVRIQPAKGLLELDLAALWQYRELIHILV
                     WRDVTVRYKQTLLGVAWAMFQPVATMLIFTVVFSVMGKIPSDGFPYPVFLYAGLLPWF
                     YISQALSRGGTSLVGEAPLISKVYFPRLILPLSATIAPLVDLFLAFIAFLGLMAWFEV
                     MPTWRILFLPLFAGLGAMITLAAGLWVSALYVRYRDVGHILPMFIQLGMFVSPILYPV
                     SKVPESWRAIYALNPVVSVVEGFRWALIPGFQVDFSMVLPSLGIVFVVFIGGLIYFRS
                     MERTFADVI"
     gene            39733..41031
                     /locus_tag="PLS35_00815"
     CDS             39733..41031
                     /locus_tag="PLS35_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17213.1"
                     /translation="MSTVAIRAEGLSKHYRLGAAVQHNTLRDQLMHRLKSLTRWGSGR
                     SEPDPSFWAVKDVSFEVKQGEVLGIIGHNGAGKSTLLKILSRITQPTKGTVDIYGRVS
                     SLLEVGTGFHSELTGRENIFLNAAMLGMRRDEVRRKFDEIVAFSGVEDFIDTPVKRYS
                     SGMYVRLAFAVAAHLEPEILIVDEVLAVGDASFQQKCLGKMEEVSRSGRTVLIVSHNM
                     GIIEGLCERAILLENGRIARIGKTQTVVEGYADAIRSQASMSIGDRPDRVGLGEILLT
                     EIDLLDKDRHSVTSAITGRDVIIRMHYRCAEGKEFRNCRVSVSVNGRKGQDLFVLSTD
                     IVDPTPLTLRGTGYVDFILPDLPLTGGAYFLQSYIESNGHAQDWIKNVVPFPVLDADY
                     YGTGNLCPPGWEANGVLVRYRWESSDDLEGSTLPLRAREK"
     gene            41180..41383
                     /locus_tag="PLS35_00820"
     CDS             41180..41383
                     /locus_tag="PLS35_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17214.1"
                     /translation="MGSAFEDGQQAQLELAGLQRTLKWTNIQREQLLDRLDLLRLDNQ
                     RLQERVEELERQLAEAKQQQVLF"
     gene            41512..42285
                     /locus_tag="PLS35_00825"
     CDS             41512..42285
                     /locus_tag="PLS35_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17215.1"
                     /translation="MTHSIKTADDVRWLLTHTQGFRGGRVMDVHLAKRWMLDEESGRD
                     VSAGRVVTVTVRYHLQGVLRIAKLSLHGVSDFSVFEQDGGDCAALGMIQVELHEGLLR
                     FWFDPQGNLYVVCEEALLEEVSVPHSDGDIHQGAAPWIFQADSGDPPTVDWLLTQLDQ
                     VGVPCIWKSTPRRAGHHGTLCWEGELVSTADVNAGPTAALTVRAYEPIDGAGFGIRLQ
                     PRLATGRFSGRLLRAVTEVVTQRYVGTCLTGERGVSSEW"
     gene            complement(42346..43032)
                     /locus_tag="PLS35_00830"
     CDS             complement(42346..43032)
                     /locus_tag="PLS35_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI17216.1"
                     /translation="MDIAKVERVYTSYSGVYDHIFGKIFHESREACVRNLRIRPEENI
                     LEVGVGTGIALEYYPKNCNITGIDLSSGMLAKARQRQSHYHLDHVRLMLMDAGKMEFP
                     DDSFDTVMAAYVVTAVPDYRKVVNEMIRVCKPGGRIIMLNHFSNGNKLIAAVEKVISP
                     LCKHIGFRTDLSLNTVLEGTDLHVARKEKVNPMKFWHLVECVNRKGAKSSNGNGSVNG
                     NGNGRHGHVS"
     gene            43289..43918
                     /locus_tag="PLS35_00835"
     CDS             43289..43918
                     /locus_tag="PLS35_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17217.1"
                     /translation="MMLAGAFAIVLFIGLIVGDWMYFTRLSSDATRYGYGIGQVQDRF
                     VALTVPKLLRAFDTNGFLSLPHGVARVFPDANRMIIRPTHRLFSMRFRTAWPLKGSIE
                     LASDSDGLRMVCTKRVPWSSSILTFLWFSLVGFGTLGFLITYVLQGGLESLGSVLLGI
                     GVTGVGLIVVAFGLLTVTVAYRIENTRLMLVYTELRDIALANEGAASRP"
     gene            complement(43967..44323)
                     /locus_tag="PLS35_00840"
     CDS             complement(43967..44323)
                     /locus_tag="PLS35_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17218.1"
                     /translation="MGEQKAPQRGTGPVESLRDEFRQHLDVFYSRLKLAAPYDSVEKA
                     LGTLTTTLKALPPEELQRLGADQTQRWAHFRQAFVDSGLIQKHRGIIAGLARSSASPD
                     LPPEFNHLLDLYRPKT"
     gene            44431..44910
                     /locus_tag="PLS35_00845"
     CDS             44431..44910
                     /locus_tag="PLS35_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17219.1"
                     /translation="MSVRSLCLLVGVLLTGLTGCSHHHGSYEHAKGDYYWGKASQDVS
                     GLVEKHVKNPETVKQVNVVMGEMIAEIKSAREQHRQFHRALYELNANYSAAPEEFTKI
                     LDELNNSRMRSSAKILGLRFKMKALLTAEEWTALSEELKRYGSRYYGKGAEGASAHQ"
     gene            complement(44927..45535)
                     /locus_tag="PLS35_00850"
     CDS             complement(44927..45535)
                     /locus_tag="PLS35_00850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNI17220.1"
                     /translation="MPTLLLIRHGETDWNRSGQVMGNQPIPLNETGEQQALACAEMLA
                     QTPIAGIVTSPVLRAVQTAHIVGRRHAAPVHHAPGLREIGVGNWINRYWKDLADDPAK
                     RDWYLHPDRARPSGGETLCEVQQRAVAAVEQALQVAQDGPYVFVSHGDVIRAVLAHYL
                     RLDLNALRQARIDHAGVSGIEVTEAATHLLFLNHRPGLPRLA"
     gene            complement(45556..46605)
                     /locus_tag="PLS35_00855"
     CDS             complement(45556..46605)
                     /locus_tag="PLS35_00855"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17221.1"
                     /translation="MSQRIRIATLLRRSICLLSFLLLACGSRESAVVSIALHPSNPNI
                     VYVATNDAVHKTRDGGATWEQFPAFTARRVTTLAIDPKLPATVYAGTMGDAIYKSPDG
                     GQHWLPHNVGLKEHVSFINEIIFHPDNTELIYLATTVGAFISKNGGREWEERMAGMKE
                     VHIVTCITMDHQHPRVLYAGTTGGTYRSEDGGTSWQKKNTGLIPEDVLNAAMALGVNM
                     LAVDPRNSEVVYAATTKGLFRSANRGESWEQIGQGLSNQFISTLALHPMDANIFYIGG
                     PDGVRKTTDGGKTWQSQNEGLTTTNIRTISLSPSDPQLLYLGTNGSGLYRSTDGGQHW
                     APIPLTGPQQPPPAS"
     gene            complement(46679..47815)
                     /locus_tag="PLS35_00860"
     CDS             complement(46679..47815)
                     /locus_tag="PLS35_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HNI17222.1"
                     /translation="MTPLRLGCTIRLLLLAVLPVTAQAVATNETVHAFDMATPGYQYR
                     FPHDHGAHERFRTEWWYYTGHVVAADGHRFGFELTFFRRGMPPDQVQTLPSRWSIQQL
                     YLAHLALTDLDAGRFYYADKVSREGLGKAGADTDRLHVWVDRWSLLTNDPAASRHQLQ
                     AATDSVAINLTVIPKKPPVIHGHDGLSRKGADPGQASHYYSLTHLATEGQIRVGTETF
                     AVTGLSWMDHEFGSADLGLNVVGWDWFSLQLSDSTELMWYSLRRADGSSDPVSSGTLV
                     LADGRAHALDAQDLNIEPLAHWTSPRSNGRYPQRWRLTSATMGLDVTVESLLADQELD
                     TARSTGVTYWEGAVSVSGQVRGAPVTGRGYVEMTGYAERFTPKL"
     gene            47995..48684
                     /locus_tag="PLS35_00865"
     CDS             47995..48684
                     /locus_tag="PLS35_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNI17223.1"
                     /translation="MFPEPEREPSNRELSGKVQPFPVPERIPLFPLPNVVFFPKTYLP
                     LHVFEPRYRQMVADAAAAGQCIGMALLKEGWEEQYEGNPPIFSIGCVGRLASVQMLPD
                     GRSNILLQGLERYEIHEEFFEKSYREARVTLKPRDGSLSLDQALRRYLMGVLDEYLKA
                     DEEASPLHSLIRPDVSDEVFVNSLSTYLDCTPLEKQFLLEAEHVPQQARRLSDLIQFK
                     LAERRGAGGWG"
     gene            48684..49655
                     /locus_tag="PLS35_00870"
     CDS             48684..49655
                     /locus_tag="PLS35_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI17224.1"
                     /translation="MSRPVAIDVSHLGKTYDKVRAVEDLSFQVYTGEIFGLLGPNGAG
                     KSTTLRILITLLNPTSGSAKIFGLDSVKDADRVRQTIGYVPQERAIDRFLTGREHLEL
                     LADLYHLSQEDAAGRIPQLLKLVELEQQADRPAKTYSGGMKRKLDIACGLLPDPKILF
                     LDEPTLGLDVQSRLRIWDHVRAMRERGITVVMTTNYLDEADQLCDRIAIIDGGRIKAL
                     GAPNELKVGLGGDLVSLTVREHDRVEKLAAAIRDLPAIRAVTVTANGLDIRVDSPEKA
                     LPAILEAANRLTCQLEFIDYHRPRLDDVFIAHTGRSIKDDHPKAEDA"
     gene            49685..50464
                     /locus_tag="PLS35_00875"
     CDS             49685..50464
                     /locus_tag="PLS35_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17225.1"
                     /translation="MKEYWQEIRALTMRWVRRLSREKFSMLFTLVQPMLFWLIFFGNL
                     FQRAADLQVTQSSSYISFLTAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMSTPIHR
                     TSVILSRFIFVTTITSLQVLVILAVAWLFGVQPVTGLAGVATILLIGMMFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMGFLAQFNPMTWAIDAVR
                     PLILQGWAEALPHVGMVIGVMVLFDALCLYGGAKAFRRAIG"
     gene            50491..51351
                     /locus_tag="PLS35_00880"
     CDS             50491..51351
                     /locus_tag="PLS35_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNI17226.1"
                     /translation="MFVNESQIRALIRLLGDEDERIVKTISGKLIDFGDCAVPLLQEA
                     EIEQPEMADRIVTVLEEIRGARLEEELRDLIAFPDDQVDLETGAFILARYAYPTLDVQ
                     AYRRQLDRMAQDVREQIGSRVSGEETVRALNRYLFTEAGFKGNTKNYYEVENSYLNCV
                     MDRRTGIPISLSTVYLLIGRRLQLPVHGIGMPGHFLVKFDSDRYKIFIDCFNGGALLT
                     EKNCARFLTEAGYGFEDRFLQKSPTRAILARMIKNLLAIYNKLDEPLKKHRLPRFVEI
                     LGCEQREEGL"
     gene            complement(51462..52220)
                     /locus_tag="PLS35_00885"
     CDS             complement(51462..52220)
                     /locus_tag="PLS35_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI17227.1"
                     /translation="MIRLTVLGSGTNVHPTRAAAGYLLQTDQTFLLDFGPRTLSNLIK
                     SGVDRHSITHILFSHFHADHFADFIPFFFDAVIFCKYQGGTRPPLTLIGPHGTATLMR
                     SMMTTFPSFDRPPFRVTIREVADRSIRIGETRITPATVTHAPKLHCVGYRVEYQGHAV
                     AYSGDSQYCESLVRLCHEADFAVLDCSFPENRPGAGHLHAGLCGRVAQEAGVNQLVLS
                     HFYPIAEQYDVRAQAKHYFPGRVRMARDLLKLRS"
     gene            complement(52217..52675)
                     /locus_tag="PLS35_00890"
     CDS             complement(52217..52675)
                     /locus_tag="PLS35_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNI17228.1"
                     /translation="MDLPDLKDDPHLKVLRPLVETYLAFWRADSRHVRSLRLTPSQFD
                     VIATLGDTKGLTCAELSTATLVTKGTLTGVLDRLEAKGLIRRTPVADDRRSTRICLTA
                     KGNKLFQTTFAAHIAFLRPFFQRALSAADADQLRTLLLRLHRSFQEKPVA"
     gene            52763..53407
                     /locus_tag="PLS35_00895"
     CDS             52763..53407
                     /locus_tag="PLS35_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI17229.1"
                     /translation="MSPAMPRHSQSGQANLTAVFLLLGLVVSGVWVWKRLSPEMQDLI
                     IDQALPLAALVLAVLVFVWMLVGKIRARRTRNQQRARLIKLFESQTAADKRLQLAFAV
                     IEVNEYRRVGLEAIAPRFQELFLTTLKRALGDKQHQVRGMAASHLGVIGDETVVPHLL
                     KALEDDHAYVRSSAALGLGRLRATAAKDKLAYVSKEDWDQTVRSRAREALERIR"
     gene            complement(53443..54276)
                     /locus_tag="PLS35_00900"
     CDS             complement(53443..54276)
                     /locus_tag="PLS35_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNI17230.1"
                     /translation="MISTVMRWQELARVWIGHWLSRPFHHLFNLIPGVEIGLSATVVT
                     RLPLTHAPLAGRRAVHLSDLHLDRYRPRHRALIRAVADLKPDWIFITGDLVNVRDGLP
                     HLLRFLTELRRLAPVYVTLGNHDHYSGIRVDEFAEQFDRRKVTLLLNQVTFIRVATGE
                     LAIVGLDDPSLHRADLRCMPSAQPGRFTLVLAHAPNILDQLRPEHHTDLTLCGHSHAG
                     QWQIPGIPTFWLPPGCHGRTHGMYEKGGHRLYVNRGLGWSVLPMRVNCTPEIVLLEWA
                     A"
     gene            complement(54273..54899)
                     /locus_tag="PLS35_00905"
     CDS             complement(54273..54899)
                     /locus_tag="PLS35_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17231.1"
                     /translation="MFFFCACTVSQARSATAAARPIGIDVFAQLTQHIKRQADTDKEA
                     FAIASQCMNFFYKQQRHKPAPPAVQQIAWIPAESSELAAADSDAQEADCRKRYPEGLD
                     AVRTDFQRTQALLSLSLTFYEFALVGDGDDNSLYNARELHDILVALNLSAEPSLGAAA
                     HLRSLTAHFDSLHEARGMEALMAGMGKLYDQGYRVTTADKAHLNRVME"
     gene            55059..56111
                     /locus_tag="PLS35_00910"
     CDS             55059..56111
                     /locus_tag="PLS35_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HNI17232.1"
                     /translation="MPTPSWLDDTVPYLTASVPALGGRIRSTPEDFCVDERPLYLPCG
                     EGEHLYIKITKRGLSTPDLVLRLSSHLHVKAQTIGVAGLKDAQAVTTQMLSLQGVKAE
                     AVAALPTDERLLSVEVLGRHRNRLRKGHHAGNQFRLVVRDVREGSEELLGRLFEELVR
                     RGVPNYFGPQRQGRSGTNFQLGAELLQDAARRNKMPRAKRIWFMNAYQSYVFNHIVAK
                     RIDSIDRVLLGDWAMKSDNGACFPVEQPEVEQLRADRFEISPTGPLFGSRAPWATGEP
                     GEIERAVIAELGTTPEVLSKAGAECGFRGERRALRVRLNDLVWSLEGSVLTLSFWLPP
                     GSYATSVLREIVKTNA"
     gene            56212..57456
                     /locus_tag="PLS35_00915"
     CDS             56212..57456
                     /locus_tag="PLS35_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNI17233.1"
                     /translation="MTALQETVFLQGHIIDSLILAKVLDTILMMGGTFDLQDVNIGAT
                     RDAPSQARIVVRAHSSRLLAEILQAIQPHGAAVERDTECRFEPAPAAGIFPDEFYATT
                     HLPTQIRLDGEWVEVEGMEMDLGICVDPAKARARTLPMGSVKAGDLIVTGREGIRVTP
                     LARPRERDVFGFMESVVSSERPHQPVIADIAQRMLKLRAGYRQGQTGTKVLLAGGPAI
                     VHAGGREALTWLIEAGFIHVLFCGNALAAHDMEAALYGTSLGYGLTAGRSVPHGHEHH
                     LRTINRIRAIGSIHEAVRSGVITEGIMAACVRQDVNMVMAGTIRDDGPLPGVVTDSMQ
                     AQGAMRAEVQGVGLALLVASTLHAIATGNLLPASVPTVCVDVNPSVPTKLADRGSFQA
                     VGLVMDAASFLKELARELGWRL"
     gene            57453..58253
                     /locus_tag="PLS35_00920"
     CDS             57453..58253
                     /locus_tag="PLS35_00920"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNI17234.1"
                     /translation="MSRLLVCPPEYFQIDYEINPWMRRENAVVPSRAVGQWQTLMHVL
                     EQDVGVGVERMQPVPGLPDLVFTANAGVVASRRVVISRFRYPERRREEVYFAQWFREH
                     GYEVLTLEESLYFEGAGDLLGFPDTWFGGYRQRSDIRSFPKLSEIFQREIIPLELIDG
                     RFYHLDTCFCPLSGGELLYFPSAFDAYGLAAIAQRIPEEQRLVVPEDEAIRFACNAVC
                     VGKRIVIPAGCPRTMRLLESRGYRTHDVELDEFMKSGGAAKCLTLALD"
     gene            complement(58287..58646)
                     /locus_tag="PLS35_00925"
     CDS             complement(58287..58646)
                     /locus_tag="PLS35_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNI17235.1"
                     /translation="MKPCPACGRALPEINRYCTYCGTGVAGDTAQKAMPQASEATRDQ
                     LNLHILYGMVTVLVVSVIMPPWETPPSQPAAFLGFHFILSPPQPDAIVSRLLLTIELT
                     TTAIAGLYLSFLFRSKG"
     gene            58768..59793
                     /locus_tag="PLS35_00930"
     CDS             58768..59793
                     /locus_tag="PLS35_00930"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNI17236.1"
                     /translation="MTRSSSQRVAVIGAGAWGTALARHLAEQQISVCVWAHEPEVVQA
                     IDQRRENVVFLPGVRLPATLTATSHLSDALIGADCVIFAVPSHVARLVLSRLGPLLPQ
                     SIPFVSATKGIEETTLELMTQVMQDVLPDHMRHSLMVLSGPSFASEVSQGKPTALCLA
                     GQDAGAVKAIQALFMTPTFRVYADDDLIGVQLGGSLKNVMALAAGVVDGLELGHNARA
                     ALITRGLAEMIRLGMAMGADPRTFYGLSGVGDLILTCTGPLSRNHSVGVRLGKGERLE
                     GILASMQAVAEGVRTAKAALGLAQRCGVDMPIVQEVNAVLFADKPCRQAVGDLMEREA
                     KEEKALS"
     gene            59790..60575
                     /gene="larB"
                     /locus_tag="PLS35_00935"
     CDS             59790..60575
                     /gene="larB"
                     /locus_tag="PLS35_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNI17237.1"
                     /translation="MNQDQLRALLMEVQQGAVAVPDALHRLRTLPYENLGFASLDHHR
                     ALRQGFPEVIFCEGKTVAQVVAIAKTLLLKNNSLLATRVEPAVARALLRMSKRATYHE
                     AARVVAIVPPKLVRRGSVLIVTAGTADIPVAEEARVTADIMGSKTDTLYDVGVAGLHR
                     LLGQQDRLHDARAIIVAAGMDGVLPSVVGGLVRQPVIAVPTSRGYGAHFGGLAALLTM
                     LNSCAAGVGVMNIDNGFGAGCLAHRINMVSEMDSPKPERAKRQ"
     gene            complement(60609..61178)
                     /locus_tag="PLS35_00940"
     CDS             complement(60609..61178)
                     /locus_tag="PLS35_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17238.1"
                     /translation="MTNSTPAPRVSLKRASTGRTFGLLLLLSGFCLLGDPSLLLAKTK
                     GKSSVPRPEPDLKILSLQAAPMPYRPQEGPFHFIATVQLPKEVDEHLMLEVSALVTSS
                     SMTSLRFLSIRQPVNEHVQTTPSANGDTPQKHVQVDLEWDGLDHNKQQIPIGSYEYEV
                     RVKLLSNGEKGQRTQMLSWPKRGKIVVKH"
     gene            61350..62558
                     /gene="larC"
                     /locus_tag="PLS35_00945"
     CDS             61350..62558
                     /gene="larC"
                     /locus_tag="PLS35_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNI17239.1"
                     /translation="MARHLHFDCFSGISGDMTLGALVDAGLPFKDLVRGLASLKIDGY
                     RLTRKRVERGAITATKVDVLIDKGFRAPLTLSQIAKILRRSGLPPAVKERSQAVFDVL
                     ADAEGKAHGVEPSHVHFHEVGVIDSFVDVVGGILGLHLLDIRRVTASAINVGSGMLQS
                     AHGALPVPGPAVAALAVGVPIYAKGPERELTTPTGMALLKTVTTDFGCLPSMQVRQVG
                     YGAGTADPAQWANVLRVFVGDEASESGAMETIVELETNLDDVNPQVYDTVFDRVFAAG
                     AVDATLAPVIMKKGRPGNVLSVLAPREHTEAVLAVLFAETTALGVRIREVQRRVLPRQ
                     FASVRVNGQDVRIKLADIRPGLSKAAPEYDDCKRVAERSGRPVKDILDEALMVYRQTL
                     GTRPRRKGRA"
     gene            62617..63630
                     /locus_tag="PLS35_00950"
     CDS             62617..63630
                     /locus_tag="PLS35_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17240.1"
                     /translation="MTVLLYVGMFVASVVITLAGCHLFTNGIEWLGKRLNISEGAVGS
                     VFAAVGTTLPETSIPIIAIFFGAGREQMEVGLGAILGAPFMLSTLVLPILALLLVLYA
                     KLGKRTATFKLNYGEVRVDISFFLVSYVLALTCAMIPSRVLHVAVAAVLLAMYVYYMK
                     LKFSAEDEESEGGDELEPLLFARRSAKPSYLVIGAQGIVGLLGLVGGAHLFVTAANSI
                     SAQLQVSPLILALLIAPLATELPEMSNSFLWLYRKKDRLAVGNVTGAMVFQGSIPVSV
                     GLLGTEWTLGTTALATMVLAVVAMSMSLLQALWSGHWRPWLLSGSALLYLGYTLFLYT
                     HGS"
     gene            63620..>64043
                     /locus_tag="PLS35_00955"
     CDS             63620..>64043
                     /locus_tag="PLS35_00955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNI17241.1"
                     /translation="MARKRSQHSLRPVLGLTMGDPAGIGPEVIAKALADKALTRLCQP
                     VVIGSRPVMERTIKWLDLPLDVVTFDPASGARVKPGQVAVADPLERPLPKFRLGVASA
                     VTGAASIAFIREAVELAQAGSVGGIVTAPINKEAMNMAG"
BASE COUNT        12783 a        17288 c        19920 g        13852 t
ORIGIN      
        1 caaggagcaa ggttcatgaa gtaccgtggc gcgtcgcaac tgatgttggg cggaatggca
       61 atggtcctgt taacggcacc gggctgtagc ggcatgaagt ggtttcagtc cggctcggat
      121 gagtcctcca attcgggttc gtctttgtcg gcgtcatccg gtgcgtcggg cgggtatgga
      181 cgcggggagg cggggcgaga cggacagtat ccctcacttg gccggcagga cggctcatcc
      241 cagcagaacg acgcatcaga tacggaaggc ggaaagttgc gcggcttttc tcctctcgtg
      301 aacggccaga tcgccggtga agaacgcctg agccggagcc cgatcggcag tatgttgacg
      361 tcaaatgagc aggacgcgaa gtgggccgcc gaaattcggc gtgaagaagc ggccgcgttg
      421 gaagccggcc tccaggacgt gttctacggg tatgatcgtt ataacgtgtc ggacaatgat
      481 ggggtggcgt cgctcaccgt caatgccggt tggttgaagg agaatcctaa ggccctcctc
      541 aagatctccg ggcactgcga cgaacgtggg acccatgatt acaatcttgt cctcggtgaa
      601 aaacgtgcca aagcggcgag aagctttttg atcgacatgg gcgtgagtgc gaagcaggtg
      661 gcgatcgtgt cctacggcaa agatcgcccc ttctgtgccg accatgacga agtgtgttat
      721 caacagaatc ggcgcggcca tatgttgctg cgcaaatagg cgagagtctc cggcgcccgc
      781 ccgcggggtg ccggggccag gtttcggcga gttgctcctt ccagcggcct ttgctcaata
      841 gcaggttccc catcgcaccc tctccgtcct tcagagaacg cccgtccatc ctgacggtcc
      901 gttaacgcct ctttcgaagc cgcagttctg cttttctgac cgttgtcagg gcccatgaat
      961 cacgtgcggg tttcccctct tgcgacagaa gggtgacaat acgcgggagc ttatggtatt
     1021 gagccacagc aggcgacaga ctttctgccg cctatttcgc cgaaggagta cgattgtgaa
     1081 ccagcgcttt gggattaccg tgaagttttt gatttcagcc gactctcccg aggaggccga
     1141 agagattatt gaatcagcct gtgaaaagat ggcgtcgtcc atccacgagg gcgatgtcag
     1201 ttacgaaggc attgaagata tcgaagagga agacgagtaa tgacgaagtc cgttccattc
     1261 gcctctgccg cctgtctccg atgccggcct cgtcacgagg ggcgatcgga ttggagaggc
     1321 tcggcagcgc cctgggccgg gttcagtggg agacagaggt gatatgacgg atactgtcga
     1381 gtcgaccgga acgcattccc gccccccggg gtttttgaac cggatccatc aatcgaataa
     1441 gcagtacctg ccgcaatggc tcggcgtgcc ggcgcacatc catcatgtcg cctaccggat
     1501 ggcgaatccg ccggtagaac ggcccaatag ccggcgggaa tttcagcatc tcctccaggc
     1561 cctcgaaatt tcggaaggag ccattgagga gcggtttggc tacgggttca agacggcgcc
     1621 caacggcgat cgtctcgtcg tcgtgtggga ggcgcacacg gagtactaca gttaccaggt
     1681 ctggcatgtg gtgggggact cgaacacgcc gctcgatttc gggccgatca cgtttcccgg
     1741 ctatcagatg ccgttgtgtc ccctcgggct gcgcgtgaat gcgctcgatc ttctgttcct
     1801 gccgcaggag ttgccgcttg atcgcgaact gccggcgcgg cttcccggcg ccatggtgta
     1861 cgggagtcgg attctgggag acgacatcgt cgcggtgacg agctttacgc ccgatgagtt
     1921 cgaccgggaa cggtacttga tttgctctac ctcggagcag gcgcttcggc aacaggtggc
     1981 gaagattgtc gatacgatcg tcgccatcga aaattattac cacttagtca tgttgccgat
     2041 gaaggcattt tcgcgggccg tcgatcaaat ccatgactat gagcaacgcc acctctatca
     2101 acgggcggtg ttgatcgagc aattgggcgg gacgaatccc ccgacgatgc agaaatggct
     2161 gacggtgctc acccaagacc tgttgcaggt gagccggctg gcagagtcga tgcgataccg
     2221 cctctccgcc tcggtgccgt acgaccgcat cgtccagagc aacctggtcg cattgcagga
     2281 gcggccttat cccccgctgc gccaaatttc agagtacgtc agttggaaga tcaccggggt
     2341 gaccgacggc taccagcagc tgctccgtcg catcgatgcc atggagaagg attttgaagc
     2401 gaccgtggcg gtgctgcgca cgcatatcga gttgcggttg caggagcaga atatccaatt
     2461 gcaggatcag aatatgaagc tgttggccag cgtggattct acgacgcggg ctcaggcgat
     2521 cctgcagcgc acagtggaaa gtctgtccgt gatcgtcatc acgtactatc tcacggggct
     2581 gggcagctat gtgctgaagg cctgttacga gatgggatgg gtgaagaatg ccaacgtggc
     2641 cacggcgatt ttcgttccga tcgccttcgc gatttccttc accctcatga ccgtagggcg
     2701 gaaaatcatc atcaagcgga tggcgaatcc cgccggtgat cccggcgggc attagattcg
     2761 gactgctacc ttctcaactt tcccgcacat catcgtcact ttttcgagag aaggtctggc
     2821 ggccatgtct ggccttgccg tgccgatcag gcagccgtca attcctgtct gtcccgctca
     2881 gatccgatgg ccgactcttc gaggacctgg ccgatcaatc gctgaagtcg gctttgttcg
     2941 tcccggcgca cgcggataaa ttcgacgcct gccttttgct cagtggccca gcgaaccttg
     3001 gccagcccca cattcagcgg tcgggcctgg tccggcgcca tgacgtgtaa ttcgagataa
     3061 tcgccgggtc ggacgggaag tgtcgtttcg atggcgcaac cgggggcgga gagattgacg
     3121 accgtgccct gtccgatcat cgaatcgccg gaaagcctga cgggataggt aacggtgacg
     3181 cgagtggatg aacgaagatc cgtgtccatg tgaaggcctc cttgccttgg gccctctact
     3241 cctgagggcg tacagacctt ttcggtaggg tatttcccga actttagcca gaaagactga
     3301 gcaatcgatc aatttcccgg ccaaggccga gcgcccggcc ggatttggta tgcggctgat
     3361 ggcaggaggg ttccccgcgg actccttgcc agggcctttt tgctggaacg cgtagaccgt
     3421 agggctggca agcgaggcag agaaggcaga tccggtccct tcccgtgaaa ggcggttgat
     3481 ttcatgggcg gaagagcgta tccatcagcg gacgtttctc gaatcgacca ccacagcagc
     3541 atcggatgaa aggttcccgt ggagcgccgc gatacaccac gctatgaaat aaaggtgccg
     3601 ctcacattct ccggccacgc catcgacggc agcgggcttg tgaccagtct ttccaaggat
     3661 ggctgccatg tcacgagcga agagtcgctt cccatgcgcg ccaacttgtc gctacatgtc
     3721 caattccccg cgccgtatga accgctgacg gtcgaagtcg cggaggtgcg atggacgaat
     3781 gccacgggct tcggcctggt gtttgtgcat gtgcatgacg aagagcatgc gcgcttgcag
     3841 cggttcatcg actggttgaa gcgcacccag aacaattaac gctgcggtcg ctcgccgttg
     3901 gccaattgcg cctgtctttc gcgaacacgc tcactcgtcc catcgtgaca ttctcttccg
     3961 aaacgatctc ctcgtgacga ttgtctgagt tgaaggcccg aactcttctc tgacacgcgt
     4021 gccggccatt cagtcagccg cgtcatgcgc gttcattatc gcgaagacgt cgtgctgcat
     4081 gagcggatcc gcacgatcag gtgtcagatt ccacgacgtc ctcaccaaaa aaatctcaac
     4141 gagactgtgc ggtggttgcg gttaggggag ggatcaggca ggataccgta tgtccccaca
     4201 acaatctcgg aactaaccag gatcctttca tggacctcgc acaactgcgc acgccctgcg
     4261 tcatgatcat ctttggcatc gatggcgacc ttgccaagcg gaagttgctg cctgctctgt
     4321 acaacctgat ggcagggcat ttcctgcccg aacaattcgc catcgtgggc ttggatcgct
     4381 cggagacgac gacggaagaa tttcgcgcga agctggatcg actcatgccc gagtatctgc
     4441 cggaaaacgc cgatcgcgcc gtatggcagg ccgtctcaca gcgtgtacat tacatggccg
     4501 gggatttcca ggaaggggcg acttaccgcc ggctgaacga cctgcttcaa ggggtcgatg
     4561 cttccgccgg gacgcagggc aattatattt tttacatggc gactatcccc ggcctgttcg
     4621 gtcggattgt gacgcacctg ggggaatacg gcctgaccgt ggaacgagac ggcacctggc
     4681 gccgcgtcat catcgagaaa ccgttcgggc atgatctgga ttcggcaaaa gccctcaatc
     4741 atgaattgca gcgagtgctg caggaacggc agatttatcg gatcgatcac tacctcggta
     4801 aagaaaccgt gcagaacatc ctggtgttcc ggtttgcgaa cgggattttt gagccgttat
     4861 ggaaccggcg gtatatcgat cacgtgcaaa tcacggtggc ggagagcctc ggggtcgaac
     4921 atcgcggccg gtattatgag caggcgggtg ctatgcggga tatggtgccg aatcatctcc
     4981 ttcagttgct ctgcttcctg gcgatggaac cgccgaactc ttttgccgcg gatgccgtgc
     5041 gcgacgagaa agccaaagtt ctgcgcggag tcgtgccgct cagttcgctt gacgtgttgc
     5101 gttttgtagc atttggccag tatggcgaag gtgcggtcga tgggaagccg gtagtcgggt
     5161 atcgcgcaga gccgcatgtg ggcgccgaat cgacgacgga gacctttgtg gcgatgaaac
     5221 tgttcatcga caactggcgc tgggccggag tgccgtttta cctgcggacg ggaaaacgaa
     5281 tgacccggcg attgaccgaa attgtggtgc aattcaagca agcgcccttc atgttgtttc
     5341 gcaagaccca agttgatcgt ttggcgccga atgtgctcgt catccgcatt cagcccgacg
     5401 agggcatttg cctgcgtttc gatgccaagg tccctggtcc cacggtgcgg atcggggagg
     5461 tgaatatgga ttttcagtac gccgactatt tcgggaatgc gccccaaacc ggctacgaaa
     5521 cgctgctcca cgacgctatg gccggcgacg cgacgctgtt tcaacgggcg gacaatattg
     5581 aattaggctg gtccgtcgtg cagatgattc tggatgtgtg gaaatctctg cccggatcag
     5641 cggccttccc gaattatcag agtggaacct ggggcccgcc tgaagccgag gcgctgttgc
     5701 ggcgcgaagg ccgtgagtgg tggaacggcg ggcaggcgta gatggtggat caacatcgaa
     5761 agcgaggtcg ttgatggcgc aggcggggat ggaggttgcg accctggcgg ggggctgctt
     5821 ctggtgtctg gaagcagtct atgatcaggt caagggtgtg caatcggtag aatccggata
     5881 tatcggcggg cagatagatg agccgacgta tgaacaggtg tgcggcgggc ggacggggca
     5941 tgccgaagcg gtacgtgtga catttgatcc cgccgtggtc agctttcgag atctgctcga
     6001 gatatttttc gtgattcacg atccgacgac gcgaaatcgg caaggaaacg atgtcggcac
     6061 ccagtatcga tcggcgatct tctaccatac gcctgaacag cagcaggccg cgcaggagat
     6121 gatcgcggcc gtgactcaag agcatctgta tcccaacccg gtggtcaccg aggtggtgcc
     6181 ggcgactcgg tggtttgaag ccgagtccta ccaccaggag tactttgccc gcaatcctct
     6241 cgaaggctat tgcacgtatg tagttgggcc gaaagtcgcg aaattccgcc agcggtttct
     6301 ggcgttgcgg aagccctgag ctccgcggtc tggctaggaa gacttgaaga gcctcaggaa
     6361 gccgtctcgc agatctttga tcatgctggg ctgctgggtg acgtggtacg gtttgataat
     6421 gagctcagcc tgcggaggac aaaacggcca ttcaatcgtg tttttgcctt ggacctgcgc
     6481 gatgatgtct tcttcatccg acccctccgc cggggtatga aggacgatca tcggatcttt
     6541 cggatcaagc agacaggttg cgtcatatcc cgcctgacgg tgttgctccg gtttaaacag
     6601 aggtacctta tgagccccgg tggaaaagac cgtgtcgcca tcgcgaatcg gcccgtcggg
     6661 ggcgagcgaa aacagcagca tcggcgtcaa cagtatcacg gctgctgcgg ccgcaatgga
     6721 ccacagcggg ggctcggctc cctgccgagg ggactgttga accgggttca tactcgcgga
     6781 tggcccccat gcgataggac ttgcttccac gtggaaactg tttcacactc acgcccccgc
     6841 accggggtca tcttacccga tgcgaaggag aaaggcgcgt gacgacagtc tccatggtgt
     6901 attgtccaat gtgctctggt agcgccaagc gagtgacccg tcatggttgg cgcgatttcg
     6961 tgatgcgtgt gatcggcttc tatccgtttc gatgtacgct gtgtctctac cgcttccgct
     7021 tgcgccgccg cacctgatgc ccgcgaggag atgcgtcttg ttcgaaatga agacagggtg
     7081 tgcggacttg ccataggtgt ccggctaccc acgatgggcc tgtcgtggaa acttgacttg
     7141 catcgcctga gggtctttca gtaggattct cccttggttg tccatctggc ctttgcgcaa
     7201 gtgagtgggg gatatgtcgt actgcggtaa gtgctccggg tatacaaaac gttcgcgccg
     7261 tcaggggctg ctggattttt tctttttcct gatcggctac tatccctacc gttgtttgcg
     7321 atgtggtcgg cgaacccatc ttcgccagcg cgtctgatcg ttccttctgc ccctgttttt
     7381 tccttcacat gtccagcgaa ggttgcgtgg cctgcgacag tgatatacct agcgctggtt
     7441 ccatgctgga actgcggcgc gtcacagatc tacagctata gcagatcctc ctcggcatcg
     7501 cttacagaag cgtcagggga ggcggcgtcc gctttcaggt cagaaaaatt gaaaagtgaa
     7561 cgatccagga gcagggaggg cgcgatgttc cccagggcgg cgaacatgct gtcgttggaa
     7621 ccgggaaaac gctgattcca gtcctgcagc atctgcttga cctgtttgcg tttcaaatcc
     7681 tcctgcgagc cgcagaggtc gcaggggatg atcggaaact gaagcagggc cgcatagcga
     7741 gccaggtccg cctctttcac ataggccaac ggtcgaatca ccacgtgacg gccgctcttg
     7801 gagcgcaact tcggcggcat cgccttcatc ttgccggtga agaaaagatt caacagcagc
     7861 gtttcgagaa tatcgtcacg atgatggcct aaggcgattt tggtcgcgcc caattccgtg
     7921 gacagccgat agagatggcc gcgccgcagg cgtgaacaaa gcgagcaggt ggtctgccct
     7981 tccggaatca atcgtttgac gacggaatag gtgtcgcgcg tttcaatgtg aaaaggaacg
     8041 ccgcggcgag tgagatactc cggcagcaca tgggcaggaa atcccggctg cttctgatcc
     8101 aggttgacgg caatgatgtc gaagtgaatc ggggcccggc tgcgtagcga cagcagaatt
     8161 tccaagagtc cgtaactgtc tttccccccc gacagacaca ccatcacctt atcgccatcc
     8221 tcaatcatgc gatagtccgc aatggcttgg cccgcgagac ggcagaggcg ggtttccacc
     8281 cgttcgatct cttcggattg tgcggctggg cgcgcagggg ctggaacggg ttttgtgctg
     8341 gtcggctgca tggccaggca ttctagctca agaaggggcg cgaatgggat aggccgaatg
     8401 ctcatcgagg gtggcgcggg aattcagcga ttcctacgga cgattcccaa ttttagacgc
     8461 cggcgtgaat cacgtcccgt cattatcacc aggcggcgtt caccgctccg agggttttcc
     8521 ctgagcctcg acgtgactcg cccagtgatc gggcagcaca attcttgcga gctgctatag
     8581 ttgaaacaga acaaaagggc gccggacggg gctttttggt ggttgcacga tggggcggac
     8641 acagattcat cgacgacaga ggtctgaacc gggcggtcgg gtgccggtct gggcagagtg
     8701 gacggacgaa cagctgctcg atcttcgcat gtgcgatctg gatctgcgcc tcgacggcac
     8761 cttctatcag gagccgatcg aacaactgta tcgcgaattg gacgcgcgcc gattggtctt
     8821 caggccgcac ttctggctct cggatgaatg gtttacgcca gacggggtac cgggtattgc
     8881 cgtcccgttt taccttgcgc acccgcggtt ggccaagctg gaagccagcc agatgttgga
     8941 agtggaaggg ggaactcacg actggtgcat gcgcatcctg cgccatgagg ccgggcatgc
     9001 gattgaaaac gcgttcttgc tgcgccgtcg ccgccgtcgg cagaaactct tcggccgctc
     9061 ctctcaaccc tatcccgaat actacacccc gagaccctat agccggagtt tcgtccgcca
     9121 cctggatgtc tggtatgccc agagccatcc ggatgaagac tttgccgaaa cgtttgccgt
     9181 gtggctggat gcggggtcgc tctggaagga gcgctaccgt ggttggccgg tcctgaagaa
     9241 actggatttc atggaacggc tcatgggtga actcgccggc gtggcgccca tggtcacggg
     9301 tcgacaacaa ctcgatccgc tgccgcgcat ctacaagacg ctgcgggacc attatgagga
     9361 aaagcgaaag cactacggaa tcggccgggc ctcctcctac gacacggacc tgaacaaatt
     9421 gttttcggcc ggagttgcct atgccaaaaa tcccagcgcc gccgagttcc ttcgccgaac
     9481 gaggaaggag atccgccgcc gggtggctga gtggacggga gagtaccaat acacgatcga
     9541 tcaagtctta gaaggcatga tcgaacggtg tcatgcgtcg aagttgcatc tgacgcaaca
     9601 gacggagcag gccaaactcg actttgcgat cctgctgacc gtgcagacca tgaattacct
     9661 gcacagcggg agacataagg tggcgttatg agacgaatgc gcgtactcgt gctcatgcat
     9721 aaggatctgg ttccgccgga gcaactcaac ggtcaggatc tggacactgc cgcgtggaaa
     9781 accgagtatg acgtggtctc gactctgcgc aaacttggcc atgacgtgca ggcgctcggt
     9841 gtgaaaagtg acctggaagt cattcgcgcg gcggtagagg attggaaacc gcacattgcc
     9901 ttcaatctac tggaagagtt cgacggcgtg gcggtgtacg accagaatgt cgtctcctat
     9961 ctcgaattga tgcgaattcc ttacaccgga tgcaacccgc gcggactcat gctggcgagg
    10021 gataaggcgt tagccaaaca agtgatgtcc taccatcgga ttccctatcc ggagtttatg
    10081 gtcgtgccgc ttcaccgcat ggtgcgtcgt cccaaatatc tgccgttccc cttgattgtg
    10141 aagtcgatca ccgaagaagc gtcgctgggc atttcgcaag cctccattgt cgacgatgac
    10201 gcgaagttgc gcgagcgggt ggccttcatt cacgacaatg tcggaaccgg agccctcgtc
    10261 gaacggtata tcgaagggcg ggagttgtat gtcggcgtga tgggtaacgc gcacttgcaa
    10321 gtgttcccgg tctgggaact ggtgatggat aagatgccgg atgagtcacg gcgcatcgcc
    10381 actcaacgcg tgaaatggag ccgcaagtac caggacaaat acgggatccg ttcatgcgaa
    10441 gcgaggaatt tgccggacgg agtgatcgat catattcagc atctggccaa acgcgtctac
    10501 cgtgcgttag gactgagcgg ctacgcgcgc atcgacatgc ggatggacca agcggggacg
    10561 gtctatgtga ttgaagccaa tccgaatcct cagatcgcca gtggtgaaga ttttgccgac
    10621 tctgccgaaa aagcgaacct cgcctacaaa gacctgctac aggaattgct gcatgtgggg
    10681 ctcaggtggc gtcctgccca agccgcgtag cggatgttga aacgggctgg ctgctgcgtt
    10741 ctcgttttgc tcaaaacttc gacgtactcg aatggtacgc ctcagctttt cgctcactgc
    10801 ggccttgcag caagacccgt ttgaacatcc gccagagcag ccgctgaaac gtctccccag
    10861 cttcgttctc gtctcgctca gagactcaac gtaccgcaag ggtacacctc gcctcctcgc
    10921 tctgctgcgg cctcgcttgg aacccgtttg agcggctgct ccaacnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnngtgat gtggtgcaga ttcaccatga gggtcgtgga
    11101 gaacgcgatc ttcggtccga cttggtattc caaagacatg cccaggggaa tgaagtgact
    11161 ggtgtcgttg cgatcgatct tcccgggtcc actgccgcga ttcagatcgg catgcagaat
    11221 acccaatccg gcaaagggca cgaggttgaa tccactgttc aggcggagat gatatttggc
    11281 gacgccggcc acgccgattt gcgtgagatc accgacgggg gtgaagaggg ccatcccgcc
    11341 gaatgaaaaa ttggggtcca tgtagtaatc gagaccgaat cccaacgcaa aggtcgtgtt
    11401 gttcgtcgtg ccggtccaga gcccgagatc ggtcgcaaag ccccaatgcg gctcctgcgg
    11461 atcggcggat tgggcggcgc aaggcgaaag aaatccgatg aaggcaaggc cggcgagaaa
    11521 aagtcggata cgtcgagcgt atgtcatagt cctacgagct ataggtggtg ggtgtatgta
    11581 gcataggccc ttgctgcgga caagcaaggg tgaccagggc gcccgcatct tagtattgtc
    11641 aatccgtcag gggctccagt atcctgccct cctgagggag gatgggcatg aaaaaagaat
    11701 ccatgacacc ggctgaagcc gcggatgcct tgtataaact gatgccgcgc cggattaccg
    11761 tagagatgtt gagtgactat gggattcagg gtgaggacgc gcatgaagat gccatgacgc
    11821 gcgaactgct gtcctttacg ctctactggg tcctggcggc tatcaacgcg cacattccgc
    11881 ggaagtaccg tgacctggtg ttccatcgcg tgatggatct gattcgcgcc gattgggaga
    11941 ccgagtacaa actcggggcg ctgaaatggg aggagtatct cgacgaggtc gaagcccgcc
    12001 gcgcccgata tgcgccggtg ggtgattacg agggcggcgc tatcgccgca tccgaagaaa
    12061 tctcagatct gcttgaaacc gaagggttga ttcaacccga agatcggccg aagttgctgg
    12121 tcctacttcc cgatctcgtg ccgctggata agtatcaaga actcctgagc cagtgtgtgt
    12181 aagccgcacc cttcgagctg tttgccgcgg cgcctagggc gaatgttgtc acaggtagcg
    12241 gtgtgactgc accttcccgt tggtgtcgag ttcgtacagc aacggcgcgc cggtgggaat
    12301 gttgagttcc agcacctgtt cgcgggtcag ctgttcgagc tgcatgacca gcgcccgcag
    12361 actgttgccg tgcgcggcga tcaggatggt ttcccccttg agtacatagg gcttgatccg
    12421 gctctcgtaa tacggcagga cccgctccgc cgtatccttc aggctttctc ctcccggagg
    12481 gcgcacgtcg taactgcgtc gccagatttt gacctgctcg tctccgaact tcttggccgt
    12541 ctcggccttg ttcagccctt gtagctcacc gtacatgcgc tcgttcagcg ccttgtcttt
    12601 ttcaacagga atggtcgtct gtccgatcgc ttcgagaatg aagcgcaggg tttcgttggc
    12661 gcgagcgagg acggaggaga aggcgcgatc gaaggtgaat cctgccagct ttttccccgc
    12721 tgctttcgct tcctcgatcc ccttcggcga gagggggacg tctacccagc cggtaaaacg
    12781 gttttccaaa ttccactgcg actcgccgtg acgaatgaga accagtttgc tcatcgtgct
    12841 tcgtctcctt gtccgaaggg atatgaccct gtcgcgacag gttatgcgat ttccccttca
    12901 ccctgtcaag ctctcttgtt tcgcacatag agcccggtcc gagtacctcg gttgcaatca
    12961 aaggtggcag tcagtaccat gccgccggtt tcccctgtaa ggttctcacc cgtggaatgt
    13021 cgcgatgccg gatgtttccc cttcggactg gtcttccttg cagcaatggt ggcaggagat
    13081 gggacgccgc ctgcaggttg aggatcgtgt cgaggcgttt ttccgtcgcg cgatgggggc
    13141 aagtcaggcc ctggcaggtg cggtgcaggt cggcggggag gtggaatata tcctcttcgt
    13201 gctggtgcgc tactggattc ccgtcgtgct tccgcctcca gggcgggaac gggcttccaa
    13261 gggtgacccg gactactggc tggaatccga acagattttg aaggccgccg cagtacgctt
    13321 gagggaacta aagccgctga tcgaactgtt gacggcaagc aatccactga gtgccggcgt
    13381 tgctgagccg caggctgcca gaccaccgat ggtggaggtc gaactagcca atatgctgca
    13441 ggggattgcg gaatcggccg gaagctacgg ggggccggat tacacctcag tgatcaagac
    13501 attcgatccc gttcccttac gtcagaccca gccgtttaag cacaataaga aaaatagtgc
    13561 cgagttgtgg gtggtctttc tgttgcgcga gcacttccgc agccttgggc tggggaagga
    13621 tcgctattgg ccattggtgg ccggtctctg cgcggctgcc gggatcgcca accctaacgg
    13681 acagccctac gggccggacg agctgaagtc gtggtggcag aagaactggc ctcgcaccta
    13741 tacccagtta gaacagacgc aggcggcgcc cgacctcagc ggagcagcgt atcagcagga
    13801 ttttgactgg ttcgtgtcct ggatcgagtg gcagcggcag cagatctcgg aagtgtagcc
    13861 ttctatcagc ccgctgctgg tttagacgtg cgtcagactg gcggggtgcg gttttgcagg
    13921 cgggcctttc ggtccatccg gcgggccctc tttaaacatc gcgacgacca atccaatttt
    13981 cagcacgagc agcccgacgc cgacccacac gacatagggt tgtacgccac ccaattcgcc
    14041 taagagctgc tcgcgggttt ctccccccgt ggcccgctcg cttttaattt tggcgagctg
    14101 ttccttgacg tgccagaaat agatgtagtt ggcgctgcct cccgcaatga cccgccagac
    14161 gatgtcggcg gacaagtcct gggtgatgta aaaggccatg gccgggccga tagcatagat
    14221 gagcgcgtac acatacatct tccggtacag aaaccagaga aatggctcga acacgaaggc
    14281 cggccagtgc cagctcaatg cgaattgcgg tccgcccggg cctgagaatt ttttgaaggt
    14341 ttccaggtac cggtcggcat tggggccgat gaaggctttc aacagttctg tatctgtctg
    14401 gggcgcgtcc tgctctgtct cgctggcggc agcgcgagcc tcgagcgcaa aaggtgcccc
    14461 gcactgatga caaaagcggg catcgtcacg gttttcttgc cggcattgcg agcaggtttt
    14521 catatcgtcc ccttatccca tccgccatct cttcccgtca agggacgccc ctgtgaaacc
    14581 ccgagcgatc cacctcgccg ccgcgctggg agctgcgaat aggcgctggt tcggttgctt
    14641 tcacctgtgc cctatgatag cttcgccgac tcacgatggg agaggtgtgt catgccgacg
    14701 ggtgagttgc gtctcaatgc cgaacagtac ctgatgaata cctaccagcg tcagccgatt
    14761 tcgatcgtgc gggggcgtgg ctcgaaggtc tatgatctgg agggccggga gtacatcgat
    14821 ttcgtggccg gcattgcggt gaatgtgctc gggcatggac atccggatct ggtgcttgct
    14881 attcagaaac aggcgcagca tctgctccat acctccaatc tgtattatac cgagccccaa
    14941 gtgcggctcg cccagaccct ggtcgaacat tcgtttgcgc agaaggtgtt tttctgcaac
    15001 agcggcgcgg aagcgaatga agcggcgatc aagctggcca gaaagtattc gtacgacaaa
    15061 tatggcgcgg atcgtcacga gatcatcacg atgacgagct cgtttcacgg gcgcacgatg
    15121 gccacgctga cggctaccgg ccaagacaaa gtccaaaagg gatttgctcc gttgatgccg
    15181 ggcttttcct atgtgccgtt taatgatctg tcagcactcg aacgagcgat cacgcccaag
    15241 acggcggcca tcatgctgga gccaatccag gccgaaggcg gagtgcatgt ggcggaacgg
    15301 ggctacctgc agggcctccg agaattgtgc cgggaacgtg acgtcctgct gattttcgac
    15361 gaggtgcaaa ccggcatcgg ccggaccggt accctctttt gttatgagca attcgggatg
    15421 cagccggaca ttatgaccct cgcgaaaggc cttggcggcg gagttccgat cggcgcctgc
    15481 ctcgcgacgg acagtgtcgc tcgtgccttc ggccccggaa cccatgcctc gacgttcggc
    15541 gggaacccct tggcctgtgc gtccgcgctc gcggtattgc gggtcttgct ggacggcaag
    15601 attctggacc agggccggcg gatgggcgag tatttggcaa aagggctggc gaccttgaaa
    15661 gaacggcatc gctgcatcaa agaggtgcgc ggcatgggcc tgttgcaggg catggaattg
    15721 gacgtcgacg gcaaaacggt tgtcgccgat tgtctgacgc gagggttatt gatcaactgc
    15781 gttggagagc gggtgctgcg tttcgtgccc gcgctcatca taacggagcg cgagatcgat
    15841 cgcctcctgg cctcgctctc acaaattttg agtcaacgaa ccacttcaac ccattaattg
    15901 tgtcgtggcg cgtatgttgc ggcgtcttcg tcggtcaact ccccggaccg gcctcgggaa
    15961 agattttctg gatttgctct cgattcccac cgacgagctg accggattgc tgcgcctcgc
    16021 ggcccagctg aaagtcaaac aacggcgcgg cgtctctcat cccctgctgc aaggccgcat
    16081 gctagggttg ctctttcaga agccgtcgac ccgaacgcgc gtgtcgttcg aggcgggcat
    16141 gaatcagctc ggcggccagg cgatggtgtt gcccatgggc gacatccaat tgtcccgcgg
    16201 cgagagcatt gccgatacgg cccatgtgtt gtcgcggtac ctcgacgcga ttgtcctgcg
    16261 aacgtttgat catgccatcg cggaagagtg ggcccgcgaa gccagcattc ctgtgattaa
    16321 cggccttacc gatctgaacc atccctgcca ggccctgtcc gatctgttga cgattcagga
    16381 gaaaaagcga cggctgaagg gcctcaagct cgcctatgtc ggcgatggga acaatgtcac
    16441 gaattcgctc atcgaagctg ccgccaagat gggtatgacg atcgcggtgg gctgtccgcc
    16501 cggctatcag ccggaccggc gtattgtgga gctgtctttg gccgaagcgg agcggacggg
    16561 ggcggtcatt gaaatcggct ccgacccctc tgtggccgtc aaggatgcgg acgtggtcta
    16621 taccgatgtc tggatcagca tgggacagga gcgggagcag gccaagcgcc tcaagaccct
    16681 tgcgccctat cagctcaatg cgcgcctgtt gaaatgcgcg aagcccgatg cgttggtgat
    16741 gcattgcctg cctgcccatc gcggcgaaga aatcagcgcc gaggtattgg atggcccgca
    16801 gtcggttgtg ttcgatcagg ccgagaaccg gttgcacatg cagaaggcga ttttggtcag
    16861 gctcctcgga aagaaaaccg cacggaagac gtaaggaaaa ggagtggtat gagtcagtcg
    16921 tcgtacaaga aagtggtgct ggcctattct ggcggattgg atacctcggt tattctcaaa
    16981 tggctggaag aagtctatgg ctgcgaagtg gtggcctttt gcgccgacct cgggcaaggc
    17041 gaagatttga aggccatcaa gaaaaaggcg cagtccctgg gtgtgaagaa ggtgtatgtc
    17101 gaggatttgc gggagacgtt tgtcaaggat cacgtgtttc ccatgttgcg tgggaatgcg
    17161 atctacgaag ggagctatct gttaggcacg tcgattgcgc ggccgctcat tgccaagcgg
    17221 caaatcgaga ttgccgccaa ggaaggcgct gcagcggtct gtcacggcgc gacgggaaag
    17281 ggcaatgacc aagtccggtt cgagctgaca tacatggcgc tgcatcccca gatcaagatt
    17341 attgcgccgt ggcgggaatg gacgatgcgc tcgcggcggg agttgattga atatgcggag
    17401 aagcacggca tccccgtcac cgcgaccaag gccaagcctt acagcatgga tatgaacctg
    17461 ttccatacga gttatgaggg gggcattctg gaagatccgt gggaggcccc gccggaggaa
    17521 attttcgtca tgtcggtttc gccggaacgc gcgccggcca aggcgcgtga agtcgagatt
    17581 gaatatgtcg ccggcaatcc ggtggcggtg gacggaaaga agatgagccc ggccgcactc
    17641 ttggggcatc tgaacaagtt gggcggcgag catggcgtcg gtcgcgtcga tctcgtggaa
    17701 aaccgctacg tcggcatgaa gtcacgcggt gtctatgaaa cgcccggtgg aaccattctg
    17761 catgcagcgc atcggggctt ggaatcgttg acgatggatc gggaagtgct gcacctgcgg
    17821 gatagtctca ttccgcgcta cgccgaactg atctattacg gatactggta tgcccccgag
    17881 cgtgaaatgc tgcaggtagc ccttgatgag gctcaaaaag atgtcacggg cactgtgcgg
    17941 gtgaagctgt acaaaggcac ctgtacggtt gttggacgga agtctccgcg ctcattgtat
    18001 cgcttggaca tggcgacgtt cgaagaagac gatgtgtacc ggcagaaaga tgccgaggga
    18061 ttcattcgtc tcaacgcact ccgcttggcg attcgcgcac aacgtaagaa gaggccgggc
    18121 aggtaatggg gaaatccaca cctgtcggcg ctcgcgggcg caagtcggcg cgtgctccgc
    18181 aacggtccgg ccgggccttg cccaaaggga aggcctgggg tggccggttt gccgagcaaa
    18241 cggaccgttt agtggagcaa ttctcctcat ccatcgccta cgaccggcga ctgtatccct
    18301 atgacatcca aggcagcatc gcccactgca aaaccctcga gcgggcaggc gttctgactg
    18361 gccgcgagtc gggtcagctt gtgcgaggct tgcaattcgt gaaggtggag ctggatggcg
    18421 ggcggtttcc cttctcgccg caggatgaag acattcacat ggcgattgag cgccgattga
    18481 cggagctgat cggacctgtc gggggaaaac tccacacagg ccgaagccgc aacgaccagg
    18541 tggcgctgga tctgcggttg tttttgcgcg atgtgttgtc caccttcatg aagcaggtgc
    18601 aggaattccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agtacattgt
    18721 ccctgatcat gatgcatctc tcgcggttga gtgaggaatt gattctctgg gcctcacagg
    18781 agtttcgcta cgtggacctg cccgatacct tttgtaccgg cagcagcatg atgccgcaaa
    18841 aaaagaatcc tgatgtgccg gagttggttc ggggaaaaac cgggcgggtg tatggccatt
    18901 tgatggggac gctgacgctg ctgaagggcc tgccgctgag ttacaacagg gatttgcagg
    18961 aagataagga agccttgttc gatgccgtgg atacgaccgg acaatcgctg gtgctgtgca
    19021 ccgagttggt gcggcggctg gccgtcaatc gggacgtcat ggctgaggcg gccgaaggtg
    19081 gtggcatgtt ggccacggaa ctggcggatt atctcgtgac gacgggggtt ccgttccggg
    19141 aggcccattc cattaccggc cagattgtgc gattctcact ggagaagcgg cggccgcttc
    19201 aacggttgac gcttaaagag ttgcagggat tttcgtcgca ctttaaggcc gatgtattga
    19261 attgtctgac agtgcgaggc gcgatcgacc gaaaagctca gatcggcggg acggcgcggc
    19321 gccgtgttga agcgagaatc aaggaactcg aaaaggcgct gaagggatga tgtccgccgg
    19381 tgcaaggcgc gcggccattc agccacacgc cttattggcg accgtatcgt tcatcttgct
    19441 ggcggggtgc ggggtcttgg gcgcgccgat tccgccggag gatgtcgggg tggcacccgt
    19501 ggtcgaacgg cagttgaggc gggaagggtt gttagcatca ggcgcgaatg ttcggggatc
    19561 agcctcgcgg ccgagcgctc cgagcgggct gacgattgaa gagaccccga ttccaccgga
    19621 tcctgatcca ctgccggttt cgccatggag gacgatgggg acgagataag cgcaggagag
    19681 tcacggattt ttctgcgaat ccccttcgcc gcggcggtcc gacacttgtt acgggccagt
    19741 ttagaattgt tgatcgagga ttattaccat gcatgacttt caatatcgga atggagaatt
    19801 gtactgtgaa gaggtgccgc tttcgcgtat tgcgaaagaa gtgggcacgc cttgttatgt
    19861 gtatagtcac cacacgttag tccgccattt tcgcgtgtac gatggcgcct ttaaaaacat
    19921 tccgcacatt gttgcctttg cgatgaaggc gaattcgaac ctcgcggtcc ttcggctcat
    19981 ggcgaaggag gggagcggag tggatatcgt ttcaggcggg gaactcttcc gtgccctgaa
    20041 ggcgggcgtg ccggcatcaa agatcgtctt tgccggggtg gggaaaaagc aggaagaaat
    20101 tcgtgacgcg ttgaaggccg acatcctcat gtttaatgtc gaatcgtctg cggagttgca
    20161 ggccattaac gaggtggcgg cttccatgga cgtgaaggcc cgggtggcgt tgcggatcaa
    20221 tcccgatatc gacccgaaga cgcatccgta catttccact ggcttgaaaa aaagcaaatt
    20281 cggtattgcg gctgatcggg cgattgaaga attcaagaat gcggcggcat tcagccatat
    20341 caaagtggta gggctgcacg cgcatatcgg atcgcaattg acgcaagtga ccccgtttgt
    20401 ggagtcgttg aagaaagtgt taacgatggt gcagacgttg gcagagcaag ggattcccct
    20461 tcggtatgtg aacatcggtg gtggcttggg cattacctat tccgatgaga cgccgccgga
    20521 accgaaagac ctggccgagg ccatttctcc actcgtgcgc gatttgaagt gtgtgctgat
    20581 catggaaccc ggccgcgtga tcgtcgggaa tgccggagtg atgcttacta aagtgttgta
    20641 tgccaaggac ggcgaagcca aacgcttcct gatcgtggat gcagcgatga acgatttgat
    20701 tcgtcccagt ctctatgatg cctatcacga tattcgtccc gtgtacgaga aggttacgca
    20761 aggggagaag aaaaccgttg atgtcgtggg ccccgtatgc gaatccggag actttttggc
    20821 aaaggatagg gtcatgccgg caatgggcgc tggtgagttg atggccgtga tgagcgccgg
    20881 tgcctacgga tttgtcatgt cctcgaatta taattcgcgc cctcgcgtcc cggaagtttt
    20941 ggtgcacgaa gggcagtatc acgtcattcg gtcgcgcgag acttacgaag acctggtccg
    21001 aggcgagcac gtgccggagt tcctttcgta agtcgggctt ttattctctt ccttgatgga
    21061 gcgtcccatg tttacagggt ctttagtggc cattgtgacg ccgttcaaaa acggcaagct
    21121 cgatgagcgt gcgctcggtg acctcatcga atggcaaatc aacagcggca cccacgggat
    21181 tgttccctgc gggacgaccg gcgaatcggc cacactaacg catgctgagc acgatcgtgt
    21241 ggtggccttc accgtggaag tcgtgagacg tcgtgttccg gtggtggcgg gaacaggatc
    21301 caatagtacc gaagaagcga ttgcattgac gaaacatgcc aaagcggccg gcgtggatgg
    21361 cgccctgctg attacgccgt attacaataa gccgacacaa gaaggcctgt ttctgcatta
    21421 caaagcggtg gctgaagcgg tcgagctccc gctcgttgtc tacaatatcc ctggccggac
    21481 cggtgtgaac atgatgcctt ccaccatcgc gcgactgacc gtctgtccga cggtggttgc
    21541 gattaaggaa ggaagcgggg cggttcagca ggcatcagaa atcattcagc tctgcgggga
    21601 gcggttgacc gtcttggcgg gggacgatgc gttgaccctg ccgatgatgg cagttggcgg
    21661 aaagggtgtc attacggtca cggccaatct cgttccgacg aagatggctg aattggtcaa
    21721 tgcatttcgt gacgggcgac tcgaggccgc gcgcgcgatg cattacgagc tgtatcctct
    21781 ctttacggcc ttgttttatg agaccaaccc cattcctgtc aaagaagcat tacatctgat
    21841 gggcaagatt gcgcctgaaa tacggttgcc gctctgcccc atgggaaatg acaataaggc
    21901 gaaactgctg actgtactga aggccgctgg cctggtgtag ttagcccgtt cgttctgaag
    21961 gatggtgcca tgatcaaggt tgttatcact ggggctgccg gacggatggg ttctcgtctc
    22021 gtgtccctgg tcaaagattc ggcatttctg acattggcgg gggctgtcga gagcaagggc
    22081 catcatgctc tgggtgagga ttccggggaa gtggccggtt gcgggcggac aggcgtgccg
    22141 atcgtcgagg atctttcaag tctgatggaa cgaggggaag tcgtcgttga ctttacgacc
    22201 cctgcggcga cgctcgggca tttaaggatc gtggctcagc atcgccgtgg aattgtggtt
    22261 gggacaacgg gattttcccc atctgaatta gacgaacttc gaagtgtgag taaacaaatt
    22321 ccatgtgtgt tctcgcctaa tatgagcgtg ggcgtgaacg tcatctacaa agtgatcgcg
    22381 gagatggcga agacacttgg cgaggactat gacatcgaag tcattgaagc gcaccaccgg
    22441 ctcaaaaagg acgcccccag cggaacggct ctcaagatgg cggaagtcct ggcacgagcg
    22501 gtcaataggg acctgggaca ggttggggtg tatgcccgca agggattgat cggtgagcgt
    22561 aaaaagggag agattggcat ccagaccatt cgtgccggag acatcgtcgg agaccacacg
    22621 gtgatgtttg gaaccatggg cgaacgtatc gagcttacgc atcgcgccag cagtcgagat
    22681 acgttcgcac ggggtgcatt acgagccgcg agatggctgg tgaagcaacc tcccggtctt
    22741 tatgacatgc tggatgtgtt gagtctcaag taagttgttt ccaacgaatt cgatctcagg
    22801 caaggtgtac ggctatttcc gcatgaggaa gcgccggcgg atcccgtccc tttgcgccta
    22861 tatttgaacc aatctgaatt gaataaggga ggagtgctgt tcgttcaaga cacggcgcaa
    22921 ctagcagttg gctctgtatg ttggccgtat gaaacggagg aggcttatcg tgcaatgggg
    22981 tggtacggaa gtccagagga ggacggtact ctgtagaggg tacgtcgcta ccccctactc
    23041 atcgcaagtg agcactctaa gatcgaatgt tactgcatcg gctcaatgtc gcgacgtgat
    23101 cggtctcggt tccgcatcgg ctgcttttcg gcgtccacag ttcaggcaag ccagcaccgt
    23161 gatcgctaaa ccagcattca gatcaaccgc ccgttccggc aacattgttc cgcggcattt
    23221 gtcacattta tgcatggatg gcttataaca ttacgctgta ggataaacca tccatctgaa
    23281 gtactgtctg ctcgagggga aaaggccctc atagccatgg aagtatctgg gccattcggt
    23341 cctagtaatg cccttaaggg attgttccat gctcgtaata tgtccaggtg gcgggaatga
    23401 atgaagggat cacccctgga catttcgtgg gtaggttagg gaagtaccac aatgtataga
    23461 ttctttctga ttgccgtact gctgatcgtc ctttactacc tgatccggag ggcgatcaga
    23521 aagattgggc aggatggtgc actggaaaaa ccaaaagatg gcagactctc cggcaaccaa
    23581 atggtacaag atcccgtttg tcgcgtcttt gtcgctcgcg agaatgccgt gaaagaaaac
    23641 attggtgggc aaacctattt tttttgcagc cgcggatgtg cggccgcatt tgagaagcag
    23701 ctttctggct agcagcctga ataggtcgag tccgagacgg tatcatcttt atcaaagaac
    23761 aagatcatct ggcattcgtt cgccttgaaa ttaaacagcg gcggtcctgc ctttcccccg
    23821 gcaattcgat tgtaggtcca cttctctcct ccgaagaagc gagtagtttt tgcgtcggga
    23881 ggccccaatt ccttttcgat ccacgcgcgg tctttccctt gataacgggt caatgaattg
    23941 tccaagtaag gattatggct gcaagcaccc acgagaaggc cgacggctag aagccaaacg
    24001 gatgcgcgca tacggctagc cctcctgatt aggtccgcgt tgaagcggac attctgcgga
    24061 tgttgatgtg tcaaacgagt ctatcatagg aaagttgacc cgttgccttg cctttgactt
    24121 gcttctagaa taaaacgtga cacaagaacg tgaaaatttg aagcgaaaag aaaggactct
    24181 ttcatgaaac tttatctcga tacggcgaac gtcaaagaaa ttcaagaagg cgccaacctg
    24241 gggctgattg atggcgtgac cacgaatccg tctttggtag ccaaggaagg gcgtagtttc
    24301 aaggaaatgt tgctggaaat ctgcaatatg gtcgatggtc cgattagtgc cgaggtcgtc
    24361 ggtgtcgaat ccgaggccat gattaaagaa ggtcgggatt tagccaaggt tcacaagaat
    24421 atcgtcgtaa aagttccgct tattcctgaa ggccttcgag ctaccaagaa gctttccgcc
    24481 gaagggattc gtgtcaatgt gaccctgtgc ttttctccca cgcaggcgct gctggcagcg
    24541 aaggccggcg cctggtgcgt atcgcccttt atcggccggc ttgatgatgt cagctcagat
    24601 ggaatggcgc tgatcaggca gattgtcacg atttacaaga attatgattt caagacccag
    24661 gtcctggtcg cgagcgtcag gcatccgcag catgtcgtcg aagcggcgtt ggcgggtggg
    24721 catatttgca cgatgcccta ttcggtgttt cagcagttgg tcaaacaccc gctgaccgat
    24781 atcggtctaa agaagttcct ggcggactgg gacgcgatgg ggaagaagta gttgcaacta
    24841 acccgagggg gacccacgcc ggttcccctc aaggcggctc gccgcatcag aaaaaatgcg
    24901 gtgaaacatg ggcctggtca atttgcccgt aaatgtattt tgctggctcg gatgatacga
    24961 gccgatcagg gatacccccc aagctaactc atatgcaacg ccatgaccga atttaggcag
    25021 gggtgagggc agttctcgcc ccatctcgcg acacgcttta aaatagtgat caaacgcaat
    25081 cttgcccaac acgacaacca cctgcacgtg ctccagcagg cgcagttcac ctaacacaaa
    25141 cgggcgacaa gctgtgaact catccggcgt gggcttgttc gcaggcggcg cgcatcgcac
    25201 agtcgcgccg atatagcagt ccgtaagagc gaggccgtca tcgacatcgg tggacgtggc
    25261 ctggttggca aagccgaatc gatgaagggc ctcatacagc caatctccgc tgcgatcgcc
    25321 tgtgaatacc cgtcccgttc gatttcctcc atgtgctgcc ggtgccaacc ccaaaacgta
    25381 cagtcgggca cgtggatccc caaatcccgg aatcggcttt ccccaataca gccactcccg
    25441 atattgccga cgcttcgtcg ccgcaacgga ttcgcggtaa ctgaccagtc gagggcattg
    25501 agtgcaggca atgatgtcag cattgagcat cgagagtgtc gacatgatgg tgaagtgtat
    25561 caaaccgacg agtttcttgt cccgtcaggg gcttgttgat agcatggggc tttcagtgtc
    25621 atgaaaggat acgtcgggca tgcggaacaa tgattggggc cttcgggcct tcgtagttgg
    25681 tgtcacgatg gtgctcggct tggcaccgtt gtttgcagaa gtctctctcg attcgaaagc
    25741 cccagcgcct tcttcgccga gcgcgagcga tgactcccgc gttcccatac ccgaacgcgg
    25801 aacaggcccc ctgtcagccg aaccagaggg tgcccctgag ctcgaagacc ggctcgtcat
    25861 ccttcctgaa atcaaacgcg agggcgagcg ctttttctta agttcattca agctgcccga
    25921 caagttgacc ttcgccgggc agtcgattcc actggataat tggcaggtgc gtgagcgcat
    25981 cgaatacgag ttctatcagt ttctcgagga tcaaggcgaa agcattatcc tggcgaagcg
    26041 aacgggccgg tgctttgccc ccgctgaaaa gcaactcgct gatgccggct tgccggatga
    26101 cctgaaatac atgttgttgg tcgagagtaa gtgcattgcc gcggcctatt ctcgagccaa
    26161 agcgtcgggt ccatggcaat tcattaactc cacgggtaag cgctacaagc tgcacaatga
    26221 acgctggctg gacgaacgtc gcaatctcga gatgtccact gaagcggcca tcaagtatct
    26281 ccgctatctt cgggaactgc agggcgactg gttcctagcc atggcgtcct ataacgcggg
    26341 cgaagaccgg gtgcgaaaac tgctcaagga acagaatgtg acggatttct ggcgcatgca
    26401 tggcccgcgg gaaaccatgc ggtatgtcgc gcgcattatt gcggcgaagg aaatctattc
    26461 ccagccggag aagtatcttg gcttgaccaa gaaggatctc tatccgccgc ttgaaacaga
    26521 gacggtgacc gtgaccatta aggaggcgca gcgccggctc acctctatcg ccgaagagta
    26581 tggcacctat ttccttgaac tgaagatgct caatccggag ttcacgaagg actacttgcc
    26641 aaaggggagt tatcaggtta aggtgccccg acaaacctgt ccgaaccgct gtttcaagca
    26701 agataaactt ccatagttcg catgatcgaa atcggcgtcc cgcagcctcg caccagagcc
    26761 cttctcgcca ggctgttgca ggcgggattg cacgcggttg atccctatga ggcggtgcgg
    26821 cgtcaggtca gcatcggacg caggcatctc accatcggct cgcagcagta ttctctcaat
    26881 caagtgaaac gtatcgtcgt ggtcggcgcc gggaaggcgt cggcccgcat ggcgcaggcg
    26941 ctcgagcacc agctggggcc gcggatcgac gccggtttgg ttgtggtgaa gtatggtcat
    27001 ggggcgccga ccaacaccat tcgtatcgtg gaggccgggc atcccattcc cgatgtcgcc
    27061 ggtcttgagg ccggccgtca ggtcatggcg gtggtaaaga cgctcacgag ggatgatctg
    27121 ctcatcgtgc ttctgtccgg tggtgcgtcg agccttctcc ctgcccccgt gtctggaatt
    27181 acgctccaag ataaacagca gacgaccaaa ttattgctgc ggtccggggc gaccattcat
    27241 gagatcaacg ccgtacgcaa gcacctgtcc agcgtcaaag gcgggcagct cgctgcggcg
    27301 actgcggcgc gcgtggccag cgtgattctc tccgacgtca tcgggaacga cctcggtacg
    27361 attggctcag ggcccaccgc gcctgatcca acgacctttc gggaggcctg ggagattgtt
    27421 gagcggtatg gggtcgtcaa aactattccg gcatctgttc ggaatcggct cagcatgggg
    27481 atgagacacc gtgcacccga gacacctaaa ccccgcgcct cggtatttcg ccgcgtcgac
    27541 aacatactca tcggagacaa tcaagccgca gtcgatgccg tcgccacggc tgcacagcaa
    27601 caagggctac gcacgttggt cctgtccacg acacttaccg gtgaagcgag ggaactcgcc
    27661 aaaatgttcg gcgccatggc tcgtgagatg gcgcggcatg gacgtcccat cccgcgcccc
    27721 tgctgcatca ttgcaggagg agaaccgacc gtcaccattc gaggggaggg aacgggcgga
    27781 cgagcccaag agtttgcgtt ggcagccgca cctgagattg cgggattgcc caacgtgtgg
    27841 gtggcgggtt ttgcgactga cgggactgat gggccgacgt ctgtggctgg tgctgtggtg
    27901 gatggccaga cggtcgtgcg ggcccgccga aaggggctgg acctgtctca ggcgttgcaa
    27961 gataacgatg cctacccatt cttcgagaaa ctgcacggtc atatcgtcac cggtcccacc
    28021 gggaccaacg tcaacgatct ttacctcctc ctcgcactct agttactatc cctctccatg
    28081 attccatcac tgattctctc tctggaagag agccgcgatc cgagtctggt cgggggcaag
    28141 gctgccggct tgaaccgttt gctcgccgct ggtttcgccg taccgcgagg attgtgtgtc
    28201 ccgacaacgg tctaccgtca tgtcctggcc gcaggcggca ttaatgccga agaagtctgg
    28261 cgaagagttc tacaggcgtc acaggaagaa cggagccagg agctggcacg agtgcacgcc
    28321 tgcctgctga agcaagcgtg gcccacagga tttcagctcg cattggaacg acggctcgtc
    28381 gagctcacgc acgatccgac aacacgatgg gctgtgcgat cctcagcaac gaacgaagat
    28441 gtcgagcaag ccagcgcggc cggcctctat agcacgtccc tcggcctgtc gtcttccgag
    28501 attttgcgag ggatctgcga ttgttggcag tccttgtgga gcgacgccgc ggtgcaatat
    28561 gccctcagga cgagcagttc aaaatctttc ccatccatgg cagtggtaat ccaaccgctg
    28621 ctgcaagcca ctaccgcagg tgtggcctac tcgatccacc cggtcacggg gcgcacttct
    28681 caggtcgtcg tcaacgccgt gcccggtctt gccgcatctc tggtcagcgg cgaggtgact
    28741 ccggaccagt atgtcgttga agtggcggaa gacgaggggc ggccaattcg gattcggcgg
    28801 cgtctgctgg ccaggaaagt gcagaaattg ctgtccaccc ctgctggact gcacaccgag
    28861 tcgatttctg aagccgggca gaagcagtcc tcattgtctg atgcgcagct tttcgagttg
    28921 gccacattgt cgaagcacat cgaagccgcg tttggacacc cgatagacct cgaatgggtg
    28981 tgggacgctg ataaggtgtg ggtggtacag gcgcgaccga tcaccgcggt gcaaccctcc
    29041 ccggcactga cgaatgatga atgcgagtgg acacgggcga atttgaaaga aaccatgccg
    29101 gaattgccga gcccgctggg cctctcgttc cttgaacggt tcatggatgc ctatatcgtt
    29161 gggccctatc ggcgcctcgg gtgcacggtt cctgagggcc tctcatccgt tcgcgtgatg
    29221 catggacggc cctatctcaa tgtcaccttg ttttacaccc tcgtcacaca attgcatggg
    29281 aacccgtcgt tcttaaccga gcagatgggt ggggaaccgt tgggctttac gccatcggtg
    29341 cgtcctttga gcgggctcgc gctggtacgc gccggtgtgc tgatgatgcg ggaatggcgg
    29401 agggttgtgc gccagggccc ggcaaacttt gtcgcgatga aagacatggc gcagacctat
    29461 cgacccgacc gtatccggaa cctctcggcg aaggagcttg cagcaacgct ggatcgtctt
    29521 gggcaatggc ttgatacgca cgaggtcacg ttcggaatcg ctggaggggt cgcacagagt
    29581 ctgcaggcaa tgggtacctt cttgcccggg tggctgggag aagactggcg agaattgctg
    29641 aacgcctctt tgcaaggcca gggaagcgtg atcagtgcgc aacacatttt gcgtctggcg
    29701 gaattagtcg aagtcgcaag tcgtgagagc gtggttcagc ggtggtttct gagagaaggc
    29761 tgggcggcct cagggtttcg cgaggccctc gccggcaccg gctttctgcg acggtttgat
    29821 gagtatctgg cggattacgg acaccgcgcg gtgggagaat cggacatcat gtcgccgcgc
    29881 atcgcggatc agcccgattc ggtcttgatg ttgttgcgga cccagttgcg tgaggggaag
    29941 gccgttccgc cgggcgatgt catgactcag caggcacagc ggcgcgaagc ggcgcttgcg
    30001 gagatttacc ggcggttcgg ctggcggcgt caccgagggg tggtttttcg ctggtggtat
    30061 cggcggctca gccgtttttg cgcgctgcgt gaggcgaatc gccatcattt actctactac
    30121 tccacggccg tgcgccatct cctccttcaa ctcggcgagc gaatggtgga gcagggaatt
    30181 ctgactcagc ctgacgacat gttttatctc accatggatg agcgggtggc actgaatgag
    30241 ggggcatcag gggattggca acggctcgta cgcacacgcc gagagcagag aacgcgtgac
    30301 caaaatgcgt ctgtgcctga caccgttcga gattgggaag cggttctcga gcagggaccg
    30361 tccatgaccg gccaagtcga tggaggactg ctgcggggca ttgccgtcag tgccggaacc
    30421 acgagtggtc cggtccgcat cgtccgttcc acacaggatt ggcagcgagt acggccgggc
    30481 gacattctcg tcgttccggt catcgatccg gggatggcgc ctctttttgg tatcgcggcc
    30541 ggcctcatcg ctgaaatggg cggcacgctg tcgcatggag ccatcatcgc gcgggagtac
    30601 ggccttccgg tcctggtcaa tgtgccctat gctaccaccc tcttgcgtga ggacgagccg
    30661 gttcacattg tgagttcgac aggcaccatc agccgcttgg tcccttgacg tttgatctgg
    30721 tttctcgtac cctctacgcg atttccctac aacgggcagg tgcgaggcat ggcggcgatg
    30781 ctctgtcttt atcaggatgc atcatgatcg atctcacctc gacaaccttt atcgcaggag
    30841 gcgtggccgg actcgggatt ggtgtcctgg tcactggatg gtgggtgaca gctcgcacgc
    30901 aatccagaat ccaggcacag ttgctcgaaa gcggggagcg tgcacaaagg gctgatgcct
    30961 tggcagtggc attgcaacag cggctcgaca ctcaacagtc cgaagttgac cagttgcggc
    31021 aggcgctggc ggaatctcaa gaaggccgga cgaaagccga aacgcggatg gaagtcatca
    31081 gtcggagcat ggaagaccaa aaggcgatgt taagccaggc gcggcaggag ctgcaggaat
    31141 ccttcgaggc cttgtccggg caagccctga aacaaaacaa tgccgcgttc ctcaacctcg
    31201 ctcgcgcctc atttgaaacc ttgcacgcgg aagccaaagg ggagttatct cagcgccacc
    31261 aagcgatcgg tgagctggtg aagccgctgg aagaatcgct ccaccgttat caggaacaat
    31321 tacaacaggc cgaacagatt cgccaacggg aatacggtgg actggaccag cagctgaagt
    31381 tcatggccga atcccatcag cggcttcagc aggaaaccag caatctcgtc aaagccttac
    31441 gggctccggc ggtgcgcggg cggtgggggg aaatgaccct ccgtcgcgtg gctgagctgg
    31501 cggggatggt ggcccattgc gattttctgg aacaggactc ggttgactca atggacggac
    31561 tgctccgtcc cgacatggtg gtgtatctgc ctgcaggccg acagatcgtg gtggatgcca
    31621 agacggtgtt ggccgcctat ctcgatgcgt acgaagccca ggatgaccag cagcggcagg
    31681 cgcatttacg tcgccatgcg aaccaggtgc gaaccaggat ggatgcgctg agcctgaaag
    31741 cctattggaa ccagttcgga cagacaccgg aattcgtcgt actgtttctg cccggtgagc
    31801 agttcctcgg cgctgcgctg gagcaggatc cttctctcat cgaggatggg tttgcccaag
    31861 ggattgttct ggccacgcct actacgttga tggcgctgtt gcgcgcggtc gcctacggat
    31921 ggaggcaaga acagttaacc gaacatgccg aacaggcagg ccggctgggc aaggatctct
    31981 acgagcggat ggccgtgctc accgaccatc tcaatgatat cggtcaggcc ttagggaaaa
    32041 gcgtcggagc ctataacaag gcggtggcct ccttggaaac ccgggtgttg cctgccgcta
    32101 gaaaatttaa agatctcggg atatcatctg agaaagacct tgcgatgctg gaatccaccg
    32161 gtatcgagcc gaggacagga tttccctttg cggccgagga tatgagaacg taagaatgat
    32221 gcagccccag gacatggtgg ccgagtttca ccgcaagttc gatatcccca tcggcgagcg
    32281 gccgtcgatt cctcctgacg ccacccggca actgcgggtg cggctgattc aagaggagtt
    32341 tgccgaactg caggaggcca tgactgcgca ggatcttccg gcggtggcga aagaactggc
    32401 tgatctgttg tatgtggtgt acggcacggc ggtgtcctac ggcgtagata tggatccggt
    32461 gttccgtgag gtgcatcgat cgaatttgag caaagtgggc ggctacaagc gggaggatgg
    32521 aaagtgggtg aaacctccga cctactctcc cgcggatgtc aaaccactgc ttgcaaaaca
    32581 gttgtcggga agcgtgggct ccgcctcgca tctggagaca aggagtcagg agtcagaagc
    32641 gtagtatgaa agagttgtat tgtccggctt gcggtacgcc gtgcgtcaga gtgacgtccg
    32701 ggacaagtct tatagagaag acgttgaacc ggctgtccat ttttcaggtt cggtgccagc
    32761 tctgcacgac tcgctttcag gctcgccggc cgggtaaccg gttgaccacg caagcattcg
    32821 atcgccgtga gtatcgtcgg ttgcccgtca actttgcggg gacgctgagg ctcgatcaca
    32881 cggcggccgg aggggtggtc accgatattt ccatgggtgg gtgtacgttg caggcgacca
    32941 caccgcttcc acgcgggacc ttcgtgacgt tcgtgctgca tgggccatcc gggcaaccgg
    33001 atatcaaagt cgaggcggcg atgatctgct cgattcagcc tcagtcgatc ggcatgaaat
    33061 ttttagaatt ccaacctgaa gataaacagc gcatggggca attggttctc gagctcctcg
    33121 cgtaccaaca agcacctccc cgcatctccg tataattcag acagagccgg atcgcttttt
    33181 atggatggat aggcctgtcc tcatgcgagg ccgggcagat tccatcgcct tcctgttcag
    33241 ggcttgcgtc tgatcgccac cggcatattg tccaggagtc tgacggttcc gaggcgaatc
    33301 gcgccgagaa gaatggcgtg gcgctggatt gtcggtaaag gttccagagt agaaggatca
    33361 cagacggcga gatattccgg tgtgactgat cgctctgtct tgatcatttg agtcatgagg
    33421 cgttgtatga gtgcccctcg ccgttcgccg ctacgaatag cagcagctcc ggcctgcagt
    33481 gcccgataga gcacaggtgc ggcgtcacgt tggcttgcag agaggtacac gttccgtgaa
    33541 ctgagcgcca acccgtcatg ttcacggatc gtcggatgaa ccctcaatgt gcctgaaaga
    33601 ttgaggtcct ttgccagttg ccggaccagt gcggcttgct gaaagtcttt ctgtccgaac
    33661 cagctgatgt cgggttggac gagcgacagg agtttcgtca ccaccgtggc cacgccctga
    33721 aaatggtgcg ggcgcgcctc gccttcccag cgccgtgcca ggctcggaac cgcgacaatg
    33781 ctctgaaaac cggccgggta catagccgtg gcggtcgggg caaagatcgc gtcgactcct
    33841 tctttcgcgc acagtgcacg gtcgagccga aattggcgtg gatatttcgc caggtcttct
    33901 gtcggaccaa actgggtcgg attgacaaaa atgctgacga ccacggcatc gcaggccaac
    33961 cgggcggctc gaaccaacgc gcgatgtccc tcatgcaagg cccccatggt cgggacaaac
    34021 ccgatcgtca ctccttctcg atggaggcca cggctccagt tggccatggc tgaagtcgtg
    34081 cggatgatct tcacgtgtga ggactctgca cagggggtgg actgcatgcc ggcctcgatc
    34141 cgtaacgtga gttcggctgg ggatcgagcc ggcggacaat ggaaggatcg gcgagtgttt
    34201 ccagaagttc tcggagagtt tgcccgagca ccggatggcg tcgatcggga tccagctcaa
    34261 ccaggggcgc gagaacgaat cgacgctgat ggagacgagg atggggaacg atgaggccgg
    34321 gatcattcag cacacagtca tcgtacagca ggagatccag atcgagcgtg cgcggcccct
    34381 tgcgattgtc ttgatctcgt ccgagtgccc gttcgatttc gcgacagatt tccagcaggc
    34441 tcttgggcgt gatgttcgtt tcgacttgca cggcgccgtt cagaaaccat gcggggccgg
    34501 gggccgctcc gtcctcaatc ggttcggtct catagagcga cgagaccgca tccagccgtg
    34561 aatgcggtag gaggccaagc agggtcaccg cccgatcaca gaaatcgatc cgattgccca
    34621 ggttagagcc gaacccgata aatgctgttg ccatagtctg aagccggctc agcgtcctgc
    34681 acgcagcacg ctgaatactg cgtcagccac ctgcggagac aggaggcacc gcaatagccg
    34741 ccacgctaga atcccacctt gcgaattcgt tcgaccgctt ccgcaagacg ctcctttgtt
    34801 gtgcacacgg tcatgcggat atagccttca ccaggcgcgc cgaagccgtt ccccggtgtc
    34861 gtgacaatgc cggctttttc cagcagatga gcggtgaagg atgccgacgt atatcccttc
    34921 ggcaccgtga cccagatgta gaaggcggcg ggaggcgcat cgacctccag cccgagtttt
    34981 ttcaggcccg ggacgagggt gtcgcgccgc tcttgataga ttttgcgtaa tccatctgtc
    35041 acggaatcat cgagcccgag ggccgtaatg ccggccgctt gcacggcttc gaacacgccc
    35101 gagtccagct ggctcttgac cctgccgagc cctgccagga cctctttgtt gccgacggcg
    35161 aatccaatgc gccacccggt catattgtag gtcttggaaa gagaatgaaa ttcgacgccg
    35221 acctctttcg cgccatcgac ttcgaggaag ctggccggcc gtttgccgtc atagaaaatt
    35281 tccgaatagg ccgcatcatg gcacacgatg atctgattct cctgcgcaaa ctcgacgaca
    35341 cgcttgaaat agtctttgct catgatgacg gacgtcggat tgttggggga gttcagccac
    35401 atcaatttgg cctgcttcgc cacatccttc gggatggcat tgagatctgg caggaagccg
    35461 ttggctttcg tgaggggcat gatgtgcgag acgccgccgc agaagctggt tccgaccgga
    35521 tagaccggat agccggggct ggggactagg acaatgtcgc ccgggtcgat gaaggcgaga
    35581 tgaacgtgcc cgatcccttc tttggagccg atcagggtca gcacctcgtt tgccggatcg
    35641 agggtgacat tgaaccgtcg cttgtaccag tcagctacgg ccttgcggaa cgacagcatg
    35701 ccctcgtagg aagggtattg atggtgtttc ggattcttgg ccgcttcata gaggctctca
    35761 atgatgggcg ccggtgtcgg cagatctggg tccccgatac caaggttgat gatatcgact
    35821 ccgcgcgcaa gcgcctcttg tttcatcttg tcgatggcgg caaaaagata gggcggcagc
    35881 gttttaatgc gggtggcgac ttcaatcgga aaaccggcca tgcgagagca ctcctttgtt
    35941 gaacctgagc ggtgacgctc agcagtgtac gataggtggc ggctggcagc cgtcaatcgg
    36001 aaatcggaag ggctagcgta ccccaggacc actctcgcaa tcaacggagt gagtgtcgga
    36061 gtggcgctcc agcccagcgg ctgatggggg agcggtcgtg aacgtcagca tcgccgtgcg
    36121 acgggttggc gccgatctca gaaaggttag acaaatgtgc cgtcctatga tgtcgtgagt
    36181 ccatggatgc aacaagttta tggcccgcag cgggtgtggt gcttgtcgcg ctcgccacgg
    36241 ccatgtttct tcctcgaact actcttcagg aggcctcgtc aacgccgcgc tatccgtcgc
    36301 tcgatgggct tcgagggtat ctggcgttgg cggtgttcgt cagccattcc tccatctggt
    36361 acttctattt gcgatcgggc acctgggacg ttcccccctc caatgtgtac acgcagctgg
    36421 gacaaggcag cgtcaccctc ttcttcatga tcaccgggtt tctcttctgg tcaaagctcc
    36481 tcgatggacg tcaccaaccg atcgactggt cgcgcctcta tctgtctcgg gcgctacgcc
    36541 taggaccgtt gtatctcgtg gcggtcgcct gtgtattgct catgtcgttt ggccgcacgg
    36601 gttttcaaat gcgtgaggcg ccggcagttg tggccgaaca gacggcacag tggctgctgt
    36661 ttacaattcc cggtatctcg tccgtcaatg gatttgcgga aacggtgccc ttggccgggg
    36721 ccgtgtggtc gctgccatat gaatggttgt tctatgccgg tttgcctctc gctgccttct
    36781 gtcttcggac aagccctccg attcagtggg tgatcgtcag tctggcggct gtggtgattc
    36841 ttgtgatggt gatgccacag gttcggttcc caatattgta tgcgtttgcc ggtggaattg
    36901 cggcggcagg gcttgtgcgt attgctgcca tccgaatgat gctgagccgt ggtgtgtggg
    36961 gcgccatcgc gatcttgtgt ctggcgaccg cgtttatggc gtttccgacg gcgtatacgg
    37021 ttccggccac gctgcttttg gccggtgggt ttctcattat tgcctgcggg aattctcttt
    37081 acggcattct cgaatggccg gcgtcgattg tcctgggcga aatggcgtac agcttgtatc
    37141 tcctgcatag tgtggtgttg tttgtaacct accggctctt gctggccgag tcggccgcca
    37201 cgctatcccc cgccgagcat tgggccatgg tgctgtgtca ggttcctgcg ctgattctcc
    37261 tctgttctgc gacgttccgc ctggtggaaa agcccggcat ggaggccgtg ccacgttggc
    37321 acgcccggct agtcgctcgc cgagcggaca caagtgccag cgcggtgccg tcgggccgcc
    37381 ggtgagacgt ggtttgcaat gggcgaacgg ttgacagacc aggcgcggct tattagagtt
    37441 cggtgtacgt tctaaccatc ctaaccacga tgccatgaaa atacttgcgc cctgcccctc
    37501 gagtcccaac tgcgtgtcga cgcaagcggc ggatcagagt cgccagatcg cgccatatcg
    37561 gtatcagcaa aatctggctg gcgcgcaaaa cacgctgaag accatcatca atcgattgcc
    37621 tcgcacccga ctcgtcgaag aaaacgacgg gtatctacac tatgaattca ccagcttctt
    37681 gctgcggttc atcgatgatg tggaatttgt ttttgatgac gcaacgaaga tgattcaatt
    37741 ccgctcggct tccaggaccg gctacagcga ccttggtgtg aatcggcggc gcatggaaga
    37801 gattcgcacc ctcgttgaag ggaaattgta gtacgaaatt ggacagccta ccccacgtga
    37861 tgcaggatta ccccggagaa gctgcgaggg caggcgattt gtggttgatt tgattgacct
    37921 gagtcgtcaa gatgcatggt agccaaggcg acatgaacat cacgggcggg cggtaattcc
    37981 taaacacacg aggcgttgtt gtgatcagac gagcgatcca cctcacagcc gtgctctccc
    38041 tagccctttc gagcgcctgt gtctctgtcc aggtggatcc gttgaccacc aaggcgtttg
    38101 aacctcgatc gggcgcggat gctgtgacga cgttacaacg cgagcctaac cacggccacg
    38161 tgcaaatcgc ccgcattgtc gctacgagtg aatatgccag tgaagatacc ttgcgtgacc
    38221 gcattctcga gcgagccaaa gaattggggg ctgacgcggt cgtattagga gacgcggatg
    38281 tccggcgtct gtcggataga ggcccgacgt ttcaatcaac gatgggcaca gccgtaccgg
    38341 gcggggtatc gaataccaat tcttaccgca gcgggtattg gaacccgttc cggatggacg
    38401 cgtggagctt tactcaaggg gctggcggcg ggcaaggttg gatgctccac atgtctgggt
    38461 tagcgattcg gtatctctca gcagaagaag tgcgagccgc acaaaagcct cccctctcta
    38521 cgccatagcc gggctccctg ccggctgaag cgactgttac ggtccttcgt cactccgtgt
    38581 atcgattgtc ccgttgaggc tggagaagtc aacgagcctg tggcttccgc gtttttaccg
    38641 gcagcaggat cgctcaggca tccattcgga agagctgttg ttcagagata gggtcaagag
    38701 agaaatgttg ttccaatggc aatgtatcga gcttgaccga gcgtcatgaa tttgacataa
    38761 caaatggtat tcagtacgag ttaggatcaa ttgtgcggct gcactaggat ttcggtctct
    38821 gcgcgtcgtc ctccagctgg acatgtctag gcctatagga accactcaaa cgaaatgtct
    38881 gttgtaaaca tcgaccagcg acatccggcg gtgctgccgc atgtgcgcat tcagccggcc
    38941 aagggtttgc tggaattgga tttggctgcc ctttggcagt atcgcgagct catccatatt
    39001 ctggtgtggc gtgatgtgac cgtgcggtac aagcagacgc tgttaggcgt ggcctgggcg
    39061 atgttccagc ctgtggcgac catgctgatt ttcacggtcg tgttcagcgt gatggggaag
    39121 attccctccg acggttttcc ctatcctgtt tttctctacg cgggactgct gccatggttt
    39181 tatatctctc aggcattgag tcggggtggg accagcttgg tgggagaggc gccactcatc
    39241 agtaaggtgt atttcccgcg gcttattctt cccctctccg cgacgattgc gccgctggtt
    39301 gatttatttc tggcattcat tgcgtttctc ggtctcatgg cctggttcga agtgatgccg
    39361 acctggagaa ttctgttcct gccgctgttc gccggattgg gggcgatgat cacattagcc
    39421 gcagggctct gggtttcggc gctctacgtc agatatcggg atgtcggtca tattcttccg
    39481 atgttcattc agttggggat gttcgtgtcg cccattctgt accctgtgag taaggtgccg
    39541 gaatcgtggc gcgccatcta tgcactcaac ccggtggtga gtgtggtgga aggattccgg
    39601 tgggcattga ttccgggctt tcaggtcgac ttttcgatgg ttcttcccag tctgggcatt
    39661 gtttttgtcg tgttcattgg cggattgatc tatttccgtt cgatggaacg cacctttgcg
    39721 gacgtgattt agatgagtac cgtcgcaatt cgagcagaag ggctatccaa gcattaccga
    39781 ctgggggcgg ctgtccagca caacacgctg cgtgatcagc tcatgcaccg gctcaagtcc
    39841 ctcactcgat ggggctccgg ccggtctgaa cccgatccct cgttttgggc agtgaaggac
    39901 gtttcgttcg aagtgaagca gggggaagtg ctgggcatca tcgggcacaa cggggctgga
    39961 aaaagcacct tactcaagat cttgtctcgc atcacgcagc ccacgaaagg caccgtggat
    40021 atctacgggc gcgtcagctc gctcttggag gtcggaacgg gctttcactc agagttgacc
    40081 ggccgggaga atatttttct gaacgcggcc atgttgggca tgcgacggga cgaagtccgg
    40141 agaaagtttg atgagatcgt cgccttctcc ggtgtggaag atttcattga tacaccggtg
    40201 aagcggtact cgagcggcat gtatgtacga ttggcctttg ccgtcgccgc gcatctcgaa
    40261 ccggaaatct tgattgtgga cgaagtgtta gcggtcggcg atgcgagttt tcagcaaaag
    40321 tgtctgggga aaatggaaga agtgagccga agcgggcgca cggtcctcat cgtcagtcac
    40381 aatatgggta tcatcgaagg gctttgcgaa cgggcgattc tgctagaaaa tgggcgcatc
    40441 gcaagaatcg gcaaaacgca gacggtagtc gaaggatatg ccgatgcgat tcgcagccag
    40501 gcgagcatgt cgatcggcga tcgcccggac cgagtcggcc tgggagagat cctgctcact
    40561 gagatcgatc tactggacaa ggatcggcac tccgtgactt ctgccataac aggacgggat
    40621 gtgatcatcc ggatgcacta ccgctgcgcg gagggtaaag aatttcgcaa ctgccgggtc
    40681 agcgtttccg tcaatgggcg gaagggacag gatctattcg tcttgtcgac cgatatcgtt
    40741 gatccgacgc ctttgaccct acgtggaacc gggtatgtgg atttcattct gcccgacctg
    40801 cctttgaccg gcggcgcgta ttttttgcag tcctatatcg agtctaacgg ccatgctcag
    40861 gactggatca agaatgtcgt gccgtttcca gtgctcgatg ccgactatta tggcacgggc
    40921 aacctctgcc cacccgggtg ggaggctaac ggcgtgctgg ttcgttatcg ctgggagtcc
    40981 agcgacgacc tcgaggggag caccttgccc ttgcgagctc gcgaaaaata agcccttcga
    41041 gagagtttcg tctcacccac gcagctgaga gcagttcccg cgcgtccctc gtcgataaat
    41101 cactaatcgc tccgcaactg gctaagagtg cgctacgatt tgcctatcac tcctgatggc
    41161 gatcgactga ggcgtacgca tgggctccgc gtttgaagac gggcagcagg cacagcttga
    41221 actggctggc ttgcagcgca cactgaaatg gaccaacatt cagcgcgaac aattgctgga
    41281 tcgtctcgat cttctccgcc ttgataatca gcgtctacag gaacgtgtag aggaattgga
    41341 gcgtcaactg gcagaagcca agcagcagca agtgctcttc tagcatccct ttccccgtta
    41401 cctcctctcc tggaaccgtc gtttccctcg cttttctccg gcgtccgcgt gatacagtgg
    41461 ttcgagaggg tgtcgataga cggcatcttg cttgaaggtt ttcacgttgc gatgacacat
    41521 tcgattaaaa cggccgacga cgttcgttgg ttgctgacgc acacgcaggg gtttcgcggc
    41581 ggtcgtgtga tggatgtgca tttggccaaa cgctggatgc tcgacgaaga gtcaggccgg
    41641 gacgtgtcgg cagggagagt cgtgacagtc accgtacgat atcatctgca aggcgtcctc
    41701 aggattgcca aactcagtct gcacggtgtc tccgactttt ccgtctttga gcaggacggc
    41761 ggggactgtg ctgctctggg aatgattcag gtggagttgc atgaaggcct cctgcgcttc
    41821 tggttcgatc cgcaggggaa cctgtacgtg gtgtgtgaag aggcgctttt ggaggaggtg
    41881 tcagtaccgc atagcgacgg ggacattcac cagggagccg cgccctggat ctttcaggct
    41941 gattccggtg accctcccac ggtggattgg ctgctgaccc aacttgatca ggtaggggtc
    42001 ccttgcatat ggaaatcgac gccgcggcgt gcgggccatc acgggacttt gtgttgggaa
    42061 ggggagttgg tgtctaccgc cgacgtgaat gccggcccca cggcagcgct gaccgtgcgg
    42121 gcctatgaac cgatcgacgg agcagggttc ggaatcagac tgcagcctcg actcgccact
    42181 ggccgcttca gcggccggtt gttgagggcg gtgactgaag tggtcactca gcggtatgtg
    42241 ggtacctgcc tgactggcga gaggggcgtt tcaagcgagt ggtgagaaaa gacgagtgag
    42301 gatttggccc ataggccgga tccaaatctc aacggaccaa gcactctaac tgacgtgccc
    42361 gtgccgcccg ttgccatttc cgttcacgct gccgttccca ttgctgctct tggcaccctt
    42421 ccggttgaca cattccacca agtgccagaa cttcatcgga ttcacttttt cttttcgggc
    42481 cacgtggagg tcggtgcctt cgagtaccgt gttcaacgag agatcggtac ggaaaccgat
    42541 gtgcttgcag agtggggaaa tgaccttctc cacggcggcg atcagcttat tgccgttgct
    42601 gaagtgattc agcatgatga tgcgcccgcc cggcttgcac acgcggatca tttcattgac
    42661 gactttgcga tagtcgggca cggcggtgac gacgtacgcc gccatcactg tgtcaaagct
    42721 atcgtcgggg aattccattt tcccggcgtc catcaacatc agccgtacat ggtccaaatg
    42781 atagtgcgac tggcgttgtc tggccttggc cagcatgcct gaggagagat cgatacccgt
    42841 aatgttgcag ttcttgggat aatattcgag ggcgattccc gttcccacgc cgacttcgag
    42901 gatgttttct tccggccgga ttcggaggtt gcgcacgcag gcttcgcgcg actcatgaaa
    42961 gatttttccg aagatatgat catagacgcc ggaataactg gtgtacacac gctcgacttt
    43021 ggcgatatcc atcgtccctc cgcaaaatca cgccgctctg cccgcacaac tctgcagtgc
    43081 agaagtgacg gcgataagaa gtgtggcgtg gaaaaatcta tgaaggcggg aacgcaactc
    43141 ggatgactcg tcctcacccg ccggataaga acgggcttaa tgtagccaat gcgcagatgg
    43201 gtagtcaaca ggttcctcct gctttttgcc tgtcgtcacc atcggttcgg acttgattct
    43261 ccgtaaagcc ctgtgggaag tttcggctat gatgctcgcc ggtgcgtttg ccatcgtgtt
    43321 gtttatcgga ttgatcgtcg gcgactggat gtactttacc cgcctctcgt ccgatgccac
    43381 ccggtacggc tatggcatcg ggcaagtgca ggaccggttt gtggccctca cggttccaaa
    43441 gctcctgcgc gcgttcgaca cgaatgggtt tttgagcttg cctcacgggg tcgcgcgggt
    43501 gtttcccgac gcgaatcgaa tgatcatccg ccccacgcat cgcctgttct cgatgcgctt
    43561 ccgcacggcc tggccgctca agggcagcat cgaattggca tcggactctg acggcctgcg
    43621 catggtgtgt acgaagcggg tgccctggtc gtcctcgatc ttgacgttcc tctggttcag
    43681 tctcgtggga ttcggaacgc tcgggttcct catcacctac gtgttgcagg gcgggctcga
    43741 gtcattggga agtgtgctgc tggggattgg cgtgaccgga gttggattga tcgtcgtcgc
    43801 ctttgggttg ctgacggtca cggtagccta tcgtatcgaa aacacgcggc tgatgctcgt
    43861 gtacacggaa ctgagagata tcgcgttggc caatgaaggg gcagcatcgc gcccctgatt
    43921 tgccaaaaga tcgcctgaat ccagcacgcc ttcgtgaacc tgtcagctaa gttttggggc
    43981 gatacagatc gagcagatga ttgaattcgg gcggcagatc cggtgaggcg ctggatcgtg
    44041 ccaggcctgc aataattccc cggtgtttct ggatcaggcc cgaatcgacg aaggcctgcc
    44101 gaaagtgagc ccatcgctgg gtctggtctg cgcccagacg ttgcagttcc tcaggcggta
    44161 acgctttgag cgtggtggtg agcgtgccca aggccttttc gacgctgtcg tagggagcgg
    44221 ccagtttgag gcggctgtaa aagacgtcga ggtgttgacg gaattcgtcc cggagtgatt
    44281 cgaccggccc tgttccgcgt tgaggggcct tttgttcgcc catacccgta ctccttgccc
    44341 tgtcaggtgt tcggaaagct tacgggagga aggcgctgcg gcgcaacgcg ttttcatgcg
    44401 acacagtcat ttttttcaat ggaggttgtg atgagcgttc gatccctgtg tcttcttgtc
    44461 ggtgtgctgt tgaccggact gaccggttgt tcccaccacc atggaagtta tgagcatgcc
    44521 aagggcgact attattgggg caaggcctct caggatgtga gcggcctggt ggaaaaacac
    44581 gtcaagaatc cggaaaccgt caagcaggtg aacgtggtga tgggcgagat gatcgccgag
    44641 atcaagtcgg ctcgggaaca acatcgccag tttcatcgcg cgctctatga gttgaacgca
    44701 aactattcag ctgcgcccga ggagttcacc aaaatcctcg acgaattgaa taacagccgg
    44761 atgcgttcct cggcaaagat cttgggcctt cggttcaaga tgaaagcctt gttgacggct
    44821 gaggaatgga ccgccttgtc cgaagaactg aagcggtatg gcagccgcta ctacggaaaa
    44881 ggcgcggagg gagcatctgc gcaccagtaa cggcgtggag tgtggttcag gccagcctgg
    44941 ggaggcctgg gcgatgattg aggaacagca ggtgggtggc ggcttcagtc acctcgatgc
    45001 cgctgacccc tgcatggtcg atgcgcgcct ggcgcagcgc attcaagtcg agccggagat
    45061 aatgggccag caccgcccga atgacgtcgc catgggacac aaacacgtag ggtccatctt
    45121 gcgccacttg cagcgcctgt tcgactgctg cgacggcacg ctgttgcact tcgcagagcg
    45181 tttcaccgcc tgaagggcgg gcccggtccg ggtgcagata ccagtcgcgc ttggcgggat
    45241 catccgccaa atccttccaa tagcgattga tccagtttcc cacgccgatc tctctcagtc
    45301 ccggggcatg atgcacgggc gctgcatggc gacgtccgac gatgtgggcg gtttgtaccg
    45361 cccgtagcac agggctggtc acgatgcctg caatgggtgt ctgcgccagc atctccgcgc
    45421 acgcgagggc ttgttgttcg ccggtctcgt tcagaggaat cggctggttt cccatgactt
    45481 gtcccgatcg attccagtcg gtttccccat gtcggatcaa caggagtgtc ggcatcggta
    45541 gtccagcgag cctcgttatg acgcgggtgg gggttgctgt ggtcccgtga ggggaatcgg
    45601 cgcccaatgt tgtcccccgt cggtcgagcg gtagagcccg ctgccattgg tgccgagata
    45661 caatagctgg ggatcactcg ggctgaggct gatcgtgcgg atattcgtcg tggtgagtcc
    45721 ctcattctgt gattgccagg tttttccgcc atcggtggtt tttcgcacac cgtccgggcc
    45781 accgatgtag aaaatatttg catccatggg atgcagcgcc agcgtactga tgaattggtt
    45841 cgacaatcct tggccgatct gttcccaaga ctctccgcga ttcgctgatc gaaacagccc
    45901 cttggtggtc gcggcgtaga ccacttcgga gttccgcgga tcaaccgcca gcatattcac
    45961 gcccaacgcc atggctgcgt tcagcacatc ctcgggaatg agcccggtat tctttttttg
    46021 ccagcttgta ccaccgtctt cactgcgata ggtgccgccc gtcgttcccg cgtacaagac
    46081 ccgggggtgt tgatggtcca tggtaatgca cgtcacgatg tgcacttcct tcatcccggc
    46141 catccgttct tcccattcgc gcccgccgtt tttggagatg aaggcgccga ccgtggtggc
    46201 gaggtaaatg agttccgtgt tgtcgggatg gaagatgatt tcattgatga acgacacatg
    46261 ctctttgagc ccgacgttgt ggggaagcca atgctggccg ccgtccggac tcttataaat
    46321 ggcgtcgccc atggtcccgg cgtacacggt ggcgggaagc ttgggatcta tggccagcgt
    46381 cgtgacgcgg cgggcggtga acgctggaaa ctgttcccag gtcgctccgc cgtcccgggt
    46441 cttgtgcacg gcatcgttcg tggcgacata gacaatattg gggtttgacg gatggagcgc
    46501 gatcgacacc acggccgatt cacgactgcc gcaggccaga agcagaaagc tgaggaggca
    46561 aatcgaccgc cgaagcaggg tcgctattcg aattcgttgg gacatcacag tggtcgcaga
    46621 cctaaccaca gcgtcgtgac tgagtcaagg tgagacatcg tcccgaaggc ctgaagcgtt
    46681 agagctttgg cgtgaaacgt tcagcatagc cggtcatctc gacatagccg cgtcctgtga
    46741 ccggtgcgcc tcgcacctga ccggagaccg acaccgcccc ttcccaatag gtcacgcctg
    46801 tgctgcgagc cgtatccaat tcctggtccg cgaggagcga ctccacagtc acatccaatc
    46861 ccatcgtagc cgaggtgagt cgccatcgct gcgggtaccg tccgttggat cgcggactgg
    46921 tccagtgggc cagtggttcg atgttgaggt cctgcgcgtc gagtgcgtgc gcgcgtccgt
    46981 cggccaggac cagcgtgcca ctcgagaccg gatcgctcga tccgtcggcg cgtcgcaagg
    47041 aataccacat cagttcggtg gaatcgctca actgaaggct gaaccaatcc catcccacca
    47101 cattcagtcc cagatcggcg gagccgaatt catgatccat ccagctgagg cccgtgacgg
    47161 caaaggtttc ggtgccgact cgaatctgcc cttcggttgc gaggtgagtg agcgaataat
    47221 agtgcgaggc ctgtcccgga tcggcgcctt ttcggctgag gccatcgtgc ccatgtatga
    47281 ccggcggttt tttcgggatg accgtgagat tgatcgccac actgtcggtc gccgcctgaa
    47341 gctggtggcg cgatgctgct ggatcattgg tgagcaggga ccagcggtcc acccacacgt
    47401 gtaaccggtc tgtgtccgcg ccggccttgc cgaggccttc gcgactgact ttgtcggcat
    47461 aataaaatcg gccggcatcc aggtcggtca gagcgagatg ggccaggtag agctgctgaa
    47521 tggaccatct ggacggaagc gtctgcacct ggtctggagg catgcctcgg cgaaagaagg
    47581 tgagttcaaa cccgaatcga tggccatcag ccgccacgac gtggccagtg taataccacc
    47641 attcagtccg aaatcgttcg tgcgcgccgt ggtcgtgtgg aaatcgatac tgataccccg
    47701 gtgtcgccat gtcgaaggcg tgaaccgtct cattcgtcgc cacggcctgt gcagtgacgg
    47761 ggagaactgc caggagtaga aggcgaatcg tgcaaccgag acgtaatggt gtcatgagat
    47821 acatcagcga ccagtcgtgc aggtcagtgc ttcaggtctg tcctgggccc actgtctttt
    47881 ttgatggaca gtcgataaaa cgccagtatc catgcatgtt ttgtgatgtt tgagagcgag
    47941 atgcggcccg gtggcagcgt tgacaatttt gcaaagcatc agctatcgtg agccatgttt
    48001 cctgagcctg aacgtgagcc gtcgaaccgg gagttgtcgg gaaaggtcca gccgtttcct
    48061 gtcccggaac gcattccctt gttccctctg cctaacgtcg ttttctttcc gaagacctat
    48121 ctcccgctcc atgtattcga gccgcgatac cgccagatgg tagcggatgc ggcggcggca
    48181 gggcaatgta tcggcatggc cttattaaag gaaggttggg aggaacagta cgaagggaat
    48241 cctcccattt tctccatcgg ctgtgtcggg cgattggcga gtgttcaaat gctccccgat
    48301 ggccggtcca acatcctgct gcaaggcctg gagcgctacg aaattcatga agagttcttc
    48361 gagaaaagct atcgtgaagc ccgcgtcacg ttgaagccgc gcgacgggtc gttatccctg
    48421 gaccaagctc tgcgacggta cctgatgggg gtgcttgatg aatatctgaa agccgatgag
    48481 gaggcctccc cgctgcacag cttgatccga cccgacgtga gcgacgaggt gttcgtgaat
    48541 tcgctctcta cctatctcga ctgtacaccg ttggaaaaac agttcctgct ggaagcagag
    48601 catgtgccgc aacaggcccg ccgtctgagc gatttgattc aattcaagct ggcggagcgg
    48661 cgcggtgccg gaggatgggg ctgatgtcgc ggccggtggc tattgacgta tcccacctcg
    48721 ggaagaccta cgacaaggtt cgggctgtcg aggacctctc gtttcaagtt tacaccggag
    48781 agatcttcgg tctgctcggt ccgaacggcg cgggcaagag caccacgctg cgaattctga
    48841 tcaccctgct caatcccacg tccggatcgg ccaaaatttt cggcctcgat tcggtgaagg
    48901 atgcggaccg tgtccggcag acaatcggct atgtgccgca ggaacgggcc atcgaccggt
    48961 tcttgaccgg ccgggaacac ctcgaactcc tcgccgacct ctaccatctc tctcaggaag
    49021 acgccgcagg acgcatcccc cagctgttaa aattggtgga actggaacag caggcggacc
    49081 gtccggcgaa gacctattcc ggtgggatga agcgcaagct ggacattgcc tgcgggttat
    49141 tgcccgatcc caaaattctg tttctcgacg agccgacact cgggctggac gtgcaaagcc
    49201 gtttgcgcat ctgggaccat gtgcgcgcca tgcgggagcg cgggattacg gtggtcatga
    49261 ccaccaacta tttggatgag gccgatcagc tgtgcgaccg gattgccatc atcgacggag
    49321 gtcgaattaa agcgctgggc gctcccaacg aactgaaagt cgggttgggc ggagacctcg
    49381 tgtcgctgac ggtgcgtgaa catgatcgcg tcgagaagct cgcggctgcc atcagggatc
    49441 ttccggccat tcgtgccgtc acggtgaccg caaacgggct cgacattcgg gtggattcgc
    49501 ccgagaaagc gttgcccgct attcttgaag cggcgaatcg cctgacgtgc cagctggagt
    49561 tcatcgatta tcatcggcct cggttggatg acgtgtttat tgcccataca gggcgatcta
    49621 tcaaggatga ccatccgaag gccgaagacg cctaactgtc gccggggagc atgcagatcg
    49681 agatatgaaa gaatattggc aggagattcg ggcgttgacg atgcgctggg tgcggcggct
    49741 gagccgggaa aagttcagca tgctgtttac gctcgtgcag ccgatgttgt tctggctgat
    49801 cttcttcggc aacctgtttc aacgcgcggc ggatctgcag gtgactcaat cgtcgagtta
    49861 cattagtttt ctgactgccg gagtggtggt catgacggtg ctcaataacg gcttggccgg
    49921 cggcgtcgac ctcctcttcg ataaggaaaa cgggtttctc gaacgcctga tgtccacgcc
    49981 gatccatcga acctccgtta tcctgagccg cttcatcttt gtcaccacca tcacgtcgct
    50041 acaggtcctc gttatcttgg cggtggcctg gctattcggc gtgcagccgg tgaccggcct
    50101 ggcgggagta gcgacgattt tgctcatcgg catgatgttc ggagtcggct taacggccat
    50161 ctcgatggcc atggcgtttt ccgtgaagag ccacggcgac ttcttttccg tgctgggctt
    50221 cctttcgctg cccatgattt tcttgagctc cgccctcgtg cccttggcgg ccatgccggg
    50281 ctggatggga tttctcgccc aattcaatcc gatgacctgg gccattgatg ccgtccgtcc
    50341 gctcattctt cagggatggg ccgaggcgtt gcctcatgtc ggaatggtca tcggcgtgat
    50401 ggtgttgttc gatgcgctgt gtctctatgg tggtgcgaag gcgttccggc gggcgatcgg
    50461 gtgagcacgg tgacgcctcg aggatcttcc atgttcgtga acgaaagcca aatccgcgcc
    50521 ctgatccggc tccttggtga cgaggatgaa cggattgtca aaacgatcag cggcaaactg
    50581 atcgatttcg gcgactgcgc tgttccgctg cttcaggagg cggaaatcga gcagccggag
    50641 atggccgatc gtatcgtcac cgtgctggag gaaattcgcg gcgcgaggtt ggaagaagag
    50701 ctccgcgatc tgatcgcctt tccggatgat caggtcgatc tggaaaccgg cgccttcatc
    50761 ctcgcgcggt atgcgtatcc cacgctggat gtgcaggcct accggcggca attggatcgt
    50821 atggcgcagg atgtccggga gcagatcggg tcccgtgtat ccggcgaaga gacggtcaga
    50881 gccttaaatc ggtatctctt caccgaggcc gggttcaaag ggaacaccaa aaattattac
    50941 gaagtcgaaa acagctatct gaattgcgtc atggaccgcc ggaccggcat tccgatcagc
    51001 ctctcgaccg tctacctgct gatcggtagg cggttgcagt tgccggtgca tggcatcggg
    51061 atgcccggcc acttcctggt gaaattcgat tcggatcggt acaaaatttt catcgattgt
    51121 ttcaacggcg gggcgctgtt gaccgaaaag aactgcgcgc ggttcctgac cgaagccggc
    51181 tacggctttg aagatcgttt cctgcagaaa agcccgaccc gagctattct ggcccggatg
    51241 atcaaaaatc tcctcgccat ctacaataag ctcgatgagc cgctgaaaaa gcaccgctta
    51301 ccacggttcg tggaaatcct gggctgtgaa cagcgcgaag aagggttgta gcaggaagct
    51361 gaacacggcc accagcgtcg ttctcgcctc attcagatcc tcaacgtagg ccgggtttct
    51421 ccccttcagg tttctcccat cgtgcagagg ctgtgtctcg actagctccg cagcttgagc
    51481 aggtcccgcg ccattctgac tcgtccagga aagtagtgtt tcgcctgcgc gcggacatca
    51541 tattgttcgg cgatcggata aaaatgtgac aggaccagct ggttgacgcc ggcttcctgg
    51601 gcgacgcggc cgcaaaggcc tgcgtgtaaa tgcccggcgc cgggccggtt ttcaggaaac
    51661 gagcaatcga gcacggcgaa atcagcctca tggcagagtc gcaccaggct ctcgcaatat
    51721 tgagaatccc ccgaataggc cacggcatgg ccctgatact cgacacggta tcctacgcaa
    51781 tgcagtttgg gggcgtgagt gacggtagcg ggggtgatgc gcgtctcgcc gatccggatg
    51841 ctgcgatcgg ccacttcgcg gatagtgact cggaagggcg ggcgatcgaa actcgggaac
    51901 gtcgtcatca tggaccgcat caaggtggcg gtgccgtgtg gaccgatgag cgtcaggggc
    51961 ggtctggtcc cgccttggta tttgcagaag atcacagcat caaagaagaa gggaataaaa
    52021 tcggcgaagt ggtcggcatg gaaatgggaa aacagaatat gggtgatgct gtggcgatcg
    52081 accccgctct ttatcaaatt ggagagtgtg cgcggtccga aatccaacag gaatgtctga
    52141 tcggtttgaa gaaggtatcc ggcggccgcg cgtgtggggt gaacattggt tcctgacccc
    52201 agcaccgtga ggcggatcat gcgaccggtt tctcctggaa acttcgatgg agacgcagga
    52261 gcagggtgcg caactgatcg gcgtcggcgg cggagagcgc gcgttggaaa aaggggcgaa
    52321 ggaacgcaat gtgggcggcg aaggtcgtct gaaacagctt gttgcccttg gcggtcaggc
    52381 aaatacgggt gcttcggcga tcatcagcca ctggtgttcg gcgaatcagt cctttggctt
    52441 ccagccgatc cagtacaccg gtgagtgtgc ccttggtcac caaggtagcc gtggagagtt
    52501 ccgcgcaggt gagtcccttc gtgtcaccga gcgtcgcgat cacgtcgaat tgtgacgggg
    52561 tgagccgaag cgaacgcaca tggcggctgt ccgcgcgcca aaatgcgaga taggtctcga
    52621 ccagggggcg gagcactttg agatgaggat cgtctttcag gtccgggagg tccatcgcgg
    52681 gcgagactag tcatccgcga gggcagctgt caagagtcgt tcacaggcct tgcgttgacg
    52741 ccgcaaggac cgccccccta tcatgtcgcc tgccatgccg cgtcactcac aatcaggaca
    52801 agccaatctg accgccgtgt ttctcctgct gggtcttgtc gtgtccggcg tctgggtgtg
    52861 gaagcgcctg tcgccggaga tgcaggatct gatcatcgat caggcgctcc cgctggcggc
    52921 cttggtgctg gcggtgctgg tcttcgtatg gatgctggtc ggaaagattc gcgcacgccg
    52981 cacgcgaaat cagcagcgcg ctcggttgat caagttgttc gagagccaga ccgcggccga
    53041 caaacgactg cagctggcgt ttgcggtgat tgaagtgaat gaataccggc gcgtgggcct
    53101 tgaggccatt gcgccacgct ttcaggaatt gtttctgacg acactcaaac gggctctggg
    53161 cgataaacaa catcaagtgc ggggcatggc ggccagccat ctgggcgtca tcggggatga
    53221 gacggtcgtg ccgcatcttc tgaaggcgtt ggaagacgat catgcctatg tgcgatcgag
    53281 tgccgccttg ggactcggcc gcctgcgcgc gacggcagcc aaggacaagt tggcctatgt
    53341 cagtaaggaa gattgggatc aaaccgtgcg gagccgggcg cgtgaggcgt tggagcgcat
    53401 tcgttaatgt gggtagggag atagagaggg acagtcagtc gttcaagcgg cccactcgag
    53461 gagcacaatt tctggtgtgc aattcacgcg catgggcagg accgaccaac ccagtccgcg
    53521 attgacgtat aaacggtggc cgcctttttc atacatcccg tgagttcgtc catgacaacc
    53581 gggcggtagc cagaatgtcg ggatgccggg aatttgccat tggccggcat ggctatggcc
    53641 gcagagcgtc agatcggtgt ggtgctcggg ccgcagctgg tccagaatgt tgggcgcgtg
    53701 ggccagcacc agggtgaaac gcccaggctg tgccgaaggc atgcagcgaa ggtctgcgcg
    53761 atggagtgac gggtcatcca gcccgacgat cgccagttcg ccggtcgcta ctcgaatgaa
    53821 cgtcacctgg ttgagcagca acgtgacctt gcggcggtcg aattgctcgg cgaactcatc
    53881 aacacggatg ccgctatagt gatcgtggtt tccgagggtg acgtagacgg gggccaggcg
    53941 gcgcagttcc gtgagaaagc gaagcagatg gggaagtcca tcccggacgt tgaccagatc
    54001 gccggtaatg aagatccaat caggcttcag gtccgccacc gctcgaatca gggcgcggtg
    54061 ccgggggcgg taacggtcca gatgtagatc gctcaaatgg acggcgcgtc gtccggccag
    54121 cggcgcatgg gtgagcggaa gccgggtcac gactgttgcc gataaaccga tctccactcc
    54181 cgggatcaaa ttgaacagat ggtgaaatgg tcgactgagc caatggccaa tccagacgcg
    54241 tgccagctcc tgccatcgca tcaccgtgga gatcattcca tcactcgatt caagtgtgct
    54301 ttgtcggcgg tggtgacgcg atagccttga tcgtagagtt tgcccatgcc ggccatgagc
    54361 gcttccatcc cacgcgcctc gtgcagggaa tcgaagtgcg cggtgagcga acgcagatgg
    54421 gccgcggcac ccagggaagg ctcggcggag aggttcaatg ccacaaggat gtcgtggagt
    54481 tcacgggcgt tgtataacga attgtcgtcg ccatcaccga ccaaggcgaa ttcgtaaaag
    54541 gtgaggctga gggagagcaa cgcctgagtg cgttgaaaat cggtgcgaac ggcatcgagt
    54601 ccttcgggat accgcttgcg gcaatccgcc tcctgtgcgt ctgaatccgc cgcagcgagt
    54661 tcagaggact ctgcgggaat ccaggcaatc tgttgcacgg cgggcggggc gggcttatgc
    54721 cgctgttgct tgtaaaaaaa attcatgcat tggctggcaa tcgcaaaggc ctctttgtcg
    54781 gtgtcagcct gacgtttaat gtgctgggtg agctgggcga agacgtcgat tccaattggt
    54841 cgggcggccg ccgtcgctga tcgggcctgc gagaccgtgc aggcgcagaa gaagaacatg
    54901 atcgcacagc tggtatgaac aacgcgtcta cgcatcggaa gtgcgtccag caggatggtt
    54961 ggcggtggag actatctcaa agcagtataa cacgggtggt cgccgtgcct aaccaaatga
    55021 catgaagtgt aatctttcag gaatcataaa ggaaactcat gccgactccc tcgtggctcg
    55081 atgatacggt accctatttg accgcgtctg tccctgcgct cggcggacgc attcgcagta
    55141 caccggaaga cttttgtgtg gatgagcgcc ccctgtatct cccctgcgga gagggcgagc
    55201 acttgtacat caaaatcacc aagcgagggc tgtccacccc ggatcttgtg ctgcgactct
    55261 cgtcccacct gcatgtgaag gcgcagacga tcggcgtggc agggctgaag gacgcgcagg
    55321 ccgtgacgac gcagatgttg tccttacagg gtgtgaaggc ggaagcggtc gcggcgttac
    55381 cgaccgacga acggctcctg agcgtcgagg tattggggcg ccatcgcaat cgattgcgta
    55441 aagggcacca tgccggcaat cagttccgtt tggtcgtgcg ggatgttcgc gagggaagtg
    55501 aggaattgtt aggccgtcta ttcgaggagt tggtgcgccg cggtgtgccc aactatttcg
    55561 gcccgcaacg gcaaggacgc tcggggacga actttcagct gggtgccgaa ttgctgcagg
    55621 atgcggcccg ccgcaacaag atgcctcgcg ccaagcgcat ctggttcatg aatgcctatc
    55681 agtcgtatgt gttcaatcac attgtggcga agcgaatcga cagtattgac cgggtgctgc
    55741 tcggtgattg ggccatgaag tccgacaacg gcgcctgctt tcctgtggag cagccggagg
    55801 tcgagcagct gcgggcggat cgattcgaaa tcagcccgac gggaccactc ttcggttcgc
    55861 gcgcaccctg ggctaccggt gagccgggcg agattgagcg agcggtcatc gctgaactgg
    55921 gaaccacgcc ggaagtgctc tcgaaagccg gagccgagtg cggatttcgc ggtgaacggc
    55981 gagccctgcg cgtgcgtctc aatgatctgg tctggtcgct ggaggggtcg gtgctgaccc
    56041 tctcgttctg gcttcccccc ggttcctatg ccacgagcgt gctacgagag atcgtgaaga
    56101 caaatgcgta gtgcgggcca tccttcctga ctaatggtag aatcatggta cgcggttgtt
    56161 acgcagggtc gataccgctg ccactgccgg agagcgttca ggagatccgt catgactgca
    56221 ctgcaggaaa cggtatttct ccaaggccac atcatcgatt cgttgatcct ggccaaggtg
    56281 ctggacacga tcctcatgat gggcggcacg ttcgatttgc aggatgtgaa cattggggcg
    56341 acgagagatg cgccctcgca ggcgcggatt gtcgtgcgcg cccattccag ccggctgttg
    56401 gccgagattc tccaagccat tcaaccccac ggcgccgcgg ttgaacggga caccgagtgc
    56461 cgtttcgagc cggctccggc ggcggggata ttcccggacg agttctatgc cacgacgcat
    56521 ttgccgaccc agatccgcct tgatggcgag tgggttgagg tcgagggcat ggagatggat
    56581 ctcggcattt gtgtcgaccc ggcaaaggct cgggccagaa cgctgcccat gggatcggtg
    56641 aaagccgggg acttgatcgt gacggggaga gaaggcatca gggtgacgcc gctggcccgt
    56701 ccccgcgaac gggatgtgtt cgggttcatg gaatctgtgg tgtcctcaga gcggccgcat
    56761 cagccggtca tcgccgatat cgcgcaacgc atgctgaaat tacgcgccgg ctatcggcag
    56821 gggcagaccg gcaccaaggt gctgcttgcg ggcggaccgg caattgtcca tgccggaggc
    56881 cgggaagcgc tgacctggtt gatcgaagcc ggatttatcc atgtcttgtt ctgcggcaat
    56941 gcgttggccg cgcacgatat ggaagccgcg ttatacggca cgtctctcgg atatggattg
    57001 actgctggac gttccgttcc gcatggacat gagcatcatt tgcggacgat caaccggatt
    57061 cgcgcgatcg gcagcatcca tgaagcggtg cgttccggtg tgattacgga aggcatcatg
    57121 gccgcctgcg tgcgccagga cgtcaacatg gtgatggccg gcacgattcg agacgacggg
    57181 ccattgcccg gcgtggtgac ggattccatg caggctcagg gggcgatgcg cgcggaagtg
    57241 cagggcgtcg gccttgcgct gttggtggcc tccacgttgc acgcgattgc gaccggcaat
    57301 ttgttgcccg cctccgtccc taccgtgtgt gtcgatgtta atccttccgt gccgacaaaa
    57361 ctggcggacc ggggcagttt tcaagccgtc ggtctcgtga tggacgccgc ctcgtttctc
    57421 aaggagctgg cgcgtgaact cgggtggcgt ctatgagccg gctgctggtg tgtccgcccg
    57481 aatatttcca aatcgattac gaaatcaatc cctggatgcg acgcgagaat gcggtggttc
    57541 cttctcgcgc ggtgggccaa tggcagacgc tgatgcacgt cctggagcag gatgtgggag
    57601 tgggcgtcga gcgaatgcag ccggttcccg gccttccgga tctcgtcttt acggccaatg
    57661 ccggggtggt ggcgagtcgc cgggttgtga tcagccggtt tcgttatccc gaacgccggc
    57721 gtgaagaggt gtacttcgcg caatggttcc gcgagcatgg gtatgaggtg ctgacgctcg
    57781 aggaatcgct ctattttgaa ggcgccggcg atctcttggg atttccggac acatggttcg
    57841 gcggatatcg ccagcgatcg gatatccggt cgtttccgaa gctgagcgaa atttttcagc
    57901 gtgagatcat tccgctcgaa ctcatcgacg gccggttcta ccacctggat acctgctttt
    57961 gtccgctcag cggcggagag ctgttgtact ttccttccgc cttcgatgcc tatggcctgg
    58021 ccgcgatcgc gcaacggatc cctgaggaac agcggcttgt cgtaccggaa gacgaagcga
    58081 ttcggtttgc ctgcaatgcg gtctgcgtcg gcaagcgtat cgtcattccg gccggctgtc
    58141 cccgcacgat gcggctcctg gaatcccgcg gctatcgcac ccatgatgtg gagctggatg
    58201 agttcatgaa gtcaggcggt gctgccaaat gcctgactct tgcactggac tgacagcact
    58261 ctcccgcctg cctgagtgcc tcaccgttac cccttcgatc ggaaaagaaa cgataggtac
    58321 agccccgcaa tcgccgtcgt cgtgagttca atcgtgagca acaaacggct gacgatggca
    58381 tcgggttgtg gcggcgaaag gatgaagtga aagccgagaa aggctgccgg ttgcgagggc
    58441 ggcgtttccc agggcggcat gatgacagac acgaccaaca ccgtgaccat gccatacagg
    58501 atgtggagat tgagctggtc tctggttgcc tcagaagctt gcggcattgc cttttgtgcc
    58561 gtgtcgccgg ccacgccggt cccacagtag gtgcagtagc gattgatttc tggcagggcg
    58621 cgtccgcagg ctggacaagg tttcatggct caggctcctc gtggacccgc agcctaccgt
    58681 gtgggcgcac ggaattccag tgtggatgcg aatgtgccgg atgcgttcgt tgacaccctt
    58741 ccagacccct tcctataatc cgcgtccatg acgcgatcat cgagtcaacg tgtagcagtc
    58801 atcggcgctg gcgcctgggg caccgcattg gcccggcatt tggccgagca acagatctcc
    58861 gtgtgtgtat gggcgcatga accggaggtc gtgcaggcca ttgaccaacg acgtgaaaac
    58921 gtcgtgtttt tgccgggagt gcggctgccg gctaccttaa ccgccaccag ccacttgtcc
    58981 gacgccctga tcggggctga ttgtgtcatc ttcgcggtgc cttcgcatgt ggcgcgactg
    59041 gttctgagcc ggcttggccc tctcctgcca cagtcgattc cattcgtgag cgcgaccaaa
    59101 ggcatcgaag aaacgacgct cgaactgatg acgcaggtga tgcaggatgt cctgcctgac
    59161 catatgcgac attcattgat ggtcttgtct ggtcccagtt tcgccagcga agtcagccag
    59221 ggaaaaccga cggcgctctg cctcgccggc caggacgccg gagccgtcaa agccatccag
    59281 gcgctcttca tgacgccgac gtttcgggtc tacgcagatg acgatctgat cggggtccag
    59341 ctcggcggat cgttgaaaaa tgtgatggcg ctggctgccg gtgtggtgga cgggctggaa
    59401 ctcggccata acgcccgtgc agccttgatc acgcgcgggt tggccgaaat gattcggctt
    59461 ggcatggcga tgggcgcaga cccgcggacg ttttacggat tgtccggggt tggagatctc
    59521 atcctgacct gtaccggccc gttgagtcgg aaccattcgg tcggggtacg gttaggcaag
    59581 ggtgaacggt tggaaggaat tctggccagc atgcaggccg tcgccgaagg cgtacgtacg
    59641 gccaaggcgg cattggggct ggcgcaacgg tgcggagtgg atatgccgat tgtgcaggag
    59701 gtcaatgcgg ttctcttcgc cgacaaaccc tgccgccagg cagtcggtga cttgatggaa
    59761 cgcgaagcga aggaagagaa ggccctctca tgaatcaaga tcagttgcgg gcgttgctca
    59821 tggaggttca gcagggcgcc gtggcggtgc cggatgcgct acatcggtta cgcacgttgc
    59881 cgtacgagaa tttaggattt gcatcgctcg atcaccaccg tgctctgcgg cagggttttc
    59941 cggaagtcat cttttgcgaa gggaaaactg tggcgcaggt ggtggctatc gccaagacac
    60001 tgttgctgaa aaataattcg ttgctcgcga cgcgggtgga acctgccgtc gcgcgcgcat
    60061 tgctccgcat gagcaagcgg gcgacatatc atgaggccgc gcgagtggtg gccattgtgc
    60121 cgcccaagct ggtgcgtcga gggtctgtgc tcatcgtcac agccggcacc gccgatattc
    60181 ccgttgccga agaggcacgt gtgacggccg atatcatggg cagcaagact gacaccttgt
    60241 acgatgtcgg tgtcgcgggg ttgcatcggt tgctcggtca acaagaccgg ttacatgacg
    60301 cgcgggcgat catcgtggcg gccgggatgg acggggtgct tccgagcgtc gtcggtggat
    60361 tagtacggca gcccgtcatc gcggtgccaa cgagtcgtgg atatggcgca catttcggcg
    60421 gcctggccgc gttactgacg atgctgaatt cttgcgcggc gggggttggg gtgatgaata
    60481 tcgataacgg gttcggcgcc ggctgcctgg cccaccgcat caacatggtg agtgagatgg
    60541 atagtcctaa gcctgaaagg gcaaagcgtc agtagaggcg gggtgtcgta tcgaagcctc
    60601 gtgctcgtct agtgtttgac cacaatcttg ccgcgtttgg gccatgacag catttgggtg
    60661 cgctggccct tctccccgtt actcaacagc ttgacccgca cttcatactc atacgatccg
    60721 atcgggatct gttgtttgtt gtgatccagc ccgtcccatt cgaggtcgac ctgcacatgt
    60781 ttttggggtg tatcgccatt ggcgcttggc gtggtctgga catgttcatt caccggctgt
    60841 cgaatcgaga gaaagcgcag cgaggtcata gaggaagagg tgacgagggc ggagacttcc
    60901 aacatgagat gttcatcgac ttccttcggc agttgcaccg tggcgatgaa atggaaagga
    60961 ccctcctgcg gtcgatacgg catgggcgca gcttgcagcg aaagaatttt taggtccggc
    61021 tcggggcgcg ggacactgct tttgcctttg gttttggcga gaagcagact tgggtctccc
    61081 agtaagcaaa agccgctgag cagaagcagc aggccgaaag tccttcctgt cgatgcgcgt
    61141 ttgaggctga cccggggagc tggtgttgag ttcgtcatgg gtgtcggtgt cgcaggtggt
    61201 ggtggctggt ggccgatgga atgcgtctct tgattgggga taacacagca tggcagggct
    61261 gtcaacgaac gagcggaatg attgctgtct gggtgcggct ccggtaagat gccgctcgtt
    61321 tgcgcgaaag agtcaattgt aggagcgacg tggcgaggca tctgcatttc gattgtttct
    61381 ccggcattag cggcgacatg acattgggcg cgttggtcga tgcggggctg ccgttcaaag
    61441 atcttgtgcg aggcctcgct tcgctgaaga tcgacggcta tcggctgacg cgtaagcgag
    61501 ttgagcgcgg agccatcacg gcgaccaagg ttgatgtcct gatcgacaaa ggctttcgcg
    61561 cgccgttaac cctgtcacaa attgccaaga ttcttcgcag gagcggcctc ccgccagcgg
    61621 tgaaagaacg aagtcaggcg gtattcgatg tgctggcaga cgccgaaggg aaggcccacg
    61681 gtgttgagcc gtcgcatgtc cattttcatg aagtgggcgt gatcgattcg tttgtggatg
    61741 tcgtcggtgg gatcctgggc ctgcatcttc tcgatatccg gcgtgtaacg gcctcagcga
    61801 tcaatgtcgg gtccggcatg ctgcagtcgg cgcacggcgc gttgcccgtg ccgggaccag
    61861 ccgtggcggc cctcgcggtc ggcgtgccga tttatgccaa ggggcctgaa cgggagctga
    61921 cgacaccgac cgggatggcc ttgctcaaaa ccgtgaccac cgatttcggc tgcctgcctt
    61981 ccatgcaggt acgccaggtg ggatatgggg cggggactgc cgacccggca cagtgggcga
    62041 atgtgttgcg cgtgttcgtc ggtgacgagg cgtccgagag cggggcgatg gaaaccatcg
    62101 tcgagctgga gacaaatctg gatgatgtga acccgcaagt gtacgacacg gtgtttgatc
    62161 gcgtatttgc cgccggcgcc gtggatgcga ccctggcgcc ggtgatcatg aagaagggcc
    62221 ggccggggaa tgtcctgtcc gtccttgcgc cgcgggaaca cacagaggcg gtcctggctg
    62281 tgctctttgc cgagacgacg gctctcggtg tgcggattcg cgaggtgcaa cgccgtgtgt
    62341 tgccgcgcca atttgcttcg gtgcgagtca acggacagga tgtgcggata aaactcgctg
    62401 atattcggcc ggggctcagc aaagccgctc cggaatatga cgattgtaaa cgcgtggcag
    62461 aacggagtgg tcgtcccgtc aaagacattc tcgacgaagc cctgatggtg tatcggcaga
    62521 cactaggcac ccgcccgaga agaaaagggc gcgcgtgatt caccagatcg tggctgttct
    62581 ctccttcccc ttttttctga tcccgcggag gcgccgatga cagttctcct gtatgtggga
    62641 atgtttgtgg cctccgtcgt catcaccctt gccgggtgtc atctcttcac gaacggcatc
    62701 gagtggctcg gaaaacgcct gaatatctcc gaaggcgctg tcgggagtgt gttcgctgcc
    62761 gtcgggacga ccttgcctga aacatcgatt ccaatcattg ccattttttt cggggccggc
    62821 cgcgaacaga tggaagtggg tctgggagcg attcttgggg cgccattcat gctgagcaca
    62881 ttagtcttgc cgattctggc gctcctgctg gtgctctatg ccaagttggg gaagcgaaca
    62941 gcaacgttca agctgaatta tggcgaggtg cgggtcgata tcagcttctt tctcgtcagc
    63001 tatgtcctgg cgctcacgtg cgcgatgatt ccctcccggg tcttgcatgt cgcggtggcg
    63061 gctgtcctgc tggccatgta cgtctattac atgaagctca agttttctgc cgaggacgaa
    63121 gaaagtgagg gcggagacga actcgagccg ctgctcttcg cgcggcgctc cgctaagccc
    63181 tcctatctgg tgatcggggc gcaaggcatc gtcgggttgc tggggttggt tggaggcgct
    63241 catctgtttg tgacggctgc caactccatt tctgcccaac tccaggtctc gccgctcatc
    63301 ctcgcattgt tgatcgctcc gctggctacg gaattgcctg agatgtccaa tagctttttg
    63361 tggttgtacc ggaaaaagga tcggctggcc gtggggaacg tgaccggtgc gatggtgttc
    63421 cagggatcga tccccgtctc cgtcggattg cttgggaccg aatggacctt gggtacaacg
    63481 gcactggcga cgatggtcct tgcggtcgtg gcgatgagca tgagcctgtt acaggccctg
    63541 tggtcagggc actggcgccc ctggttgctc agcggcagcg cgctgctgta ccttgggtac
    63601 acgttattct tgtacaccca tggctcgtaa gcgttcacaa cactcgctcc gccccgtgtt
    63661 gggtctcacg atgggcgacc cggccggcat tggccccgag gtcattgcga aggccttggc
    63721 cgacaaggcg ctcacgcggc tctgtcagcc ggtggtcatc ggctcccgcc cggtcatgga
    63781 acgcacgatc aagtggttgg atttgccgtt ggacgtggtg acgtttgatc cggcgagcgg
    63841 tgcacgagtg aagcccggtc aggtcgctgt cgccgatccg ttggaacgtc cgctgccgaa
    63901 atttcgcctg ggggtggcgt cggcagtgac cggggcggcc tccatcgcat ttatcagaga
    63961 agccgtcgaa ttggcgcagg cggggagtgt gggcggaatc gtcactgcgc ccatcaataa
    64021 agaagcgatg aacatggccg gct
//
LOCUS       DAOQEW010000004        56225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_91_length_56225_cov_11.714314, whole genome shotgun sequence.
ACCESSION   DAOQEW010000004 DAOQEW010000000
VERSION     DAOQEW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 56225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_56225_cov_11.714314"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLS35_00960"
     CDS             complement(<1..670)
                     /locus_tag="PLS35_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNI17242.1"
                     /translation="MKVLPKQCPRLHVIQIGCGGIGAFLGIHVARLARECFRLFDTVM
                     VTFYDGDTIEEKNLRRQNFCQAEIGHNKAEALAFRLSTAWGIPIRACPRHFSGKEAHP
                     EYDTLTILLGCVDTARARRSLHHALARWNTRNDAQAWLIDGGNLTTHGQVLVGNVVTD
                     FNPHESFQLRTVCQSLPAPALVHPELLKRERRPVNTQQRSCAELALGDPQSLTINSII
                     ASHMA"
     gene            complement(1390..2073)
                     /locus_tag="PLS35_00965"
     CDS             complement(1390..2073)
                     /locus_tag="PLS35_00965"
                     /inference="COORDINATES: protein motif:HMM:NF025815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17243.1"
                     /translation="MRGASSDQHDASLHFIGGQLLLEYKDGEAVIRKCISPEAARNAF
                     SSAGIDSDWLPEHVCRYGIGPRGPWLLLRFAPGRYQIPLADPITLSGGGEPHTMLAVP
                     MPGLLFLGYGTHYYVWAYKLWKCAATKLFKAPLANVYPDGAICFGNVHPRVAHGNTIT
                     NSWRLFWDSNFSDHLANGKSNAHPDNILPFLAKLHSTEAQDYPLDDLEPAHLSIATII
                     RQLMQSGKG"
     gene            complement(2869..3306)
                     /locus_tag="PLS35_00970"
     CDS             complement(2869..3306)
                     /locus_tag="PLS35_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17244.1"
                     /translation="MVTLQLEGQEIQLPPEIAISDDAVRKAIAPLFPQAAHATIQRTT
                     DTNGMTTIRLTKQAGTKGNYHVILRQLIESPRHVNPALTLYQAYQAESQPTQTTVESI
                     LLTQQKLRLAIEHGEKEYKETATIKERLRNTPGSPSRLLPPGF"
     gene            complement(3320..3853)
                     /locus_tag="PLS35_00975"
     CDS             complement(3320..3853)
                     /locus_tag="PLS35_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17245.1"
                     /translation="MKTEAVPAPPPPRLEEEDDEDEQDPIASFEQDSNDFQRATISIV
                     MTLLPTDHHPDGRLTLIGVRSHNLPPQLTTARFNDLLPLPESLSQALASWEQTFTAAL
                     TARKTQRDAKAAEQQAKTVKQEKKREAERQARKPKVAKPAAAKAPPVALTPKPDREQH
                     THDSTSNTALPQAGLFE"
     gene            complement(4139..5800)
                     /locus_tag="PLS35_00980"
     CDS             complement(4139..5800)
                     /locus_tag="PLS35_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17246.1"
                     /translation="MPAGIRTSLTRVYTYPAYLTFSSYAALPAAVREEADQMQSLWNA
                     MVDVYEVAHRQQERLHKQFLQTSRTTTRAAGGTRSIGDPFECIKQLRDQNDSLEDETL
                     LALSRTYRTAAAEYEASEHHNIDRCLPYPESMKQELSQITKRLHDTLHQLARTSPLSR
                     SHSNAVLERMKTAIRRVPVGNLPPSKETGPPTSLFFTHDFGSQGIRIQSLTRADVTQS
                     GSSFAANRLLTLHPVSQFAEDTDPRERGQRDFCRTEGELFVRNVPIPFEIILTPLLPK
                     DALLKQVSLIGSQQRPQTSVNMDSSNACLRNDTTWQWYLQFTLIVPPTQQSEPKKYGM
                     IALDFDHRLINDANPLVRVGEWVSDTGKHESVFFPARVVKNLIAAYEAREALNVQHLA
                     LKQLFVHFPFAGALADMVKEPIHSDVPLSRKRLRWIAHQAVKQAYSGPLTSIIREILS
                     QIRDTRIDVVRRRRQWRRDRAEFYDKLAHRLSREAGTIVLTPVLSAGRSGAEGSSTST
                     TRSAPLAQALAHPINMQEFIHRLQHAAPIHRTTLHFVQSMHALIA"
     gene            6688..7026
                     /locus_tag="PLS35_00985"
     CDS             6688..7026
                     /locus_tag="PLS35_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17247.1"
                     /translation="MSIAQLPNSVFEDAPLSCVFHSAQDNLYDVGKIVGLSMHHCHVE
                     SQAEVTPGMTLSLFVVLPTKRRALVLDEALVTWVRTEEFGVRLKTIPGEDAAYLESYL
                     AGHKVDAGLC"
     gene            7020..7502
                     /locus_tag="PLS35_00990"
     CDS             7020..7502
                     /locus_tag="PLS35_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17248.1"
                     /translation="MLNIHRIDHEASHTHCWRVTVQRRTRIYVRSFSDGRYGGAEQAL
                     VAATTYRDALIRSNPPLSKPVYCAILKKTNRSGISGLSCIDRWEFSKGRRVHRLYWDV
                     QWPIENCQSRHKKFSILKYGEDGAYRRALKARAAALKAISSQTFSPFASRVAANSAAA
                     "
     gene            complement(7526..8098)
                     /locus_tag="PLS35_00995"
     CDS             complement(7526..8098)
                     /locus_tag="PLS35_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNI17249.1"
                     /translation="MNPNFRNADAFTKLVLQPYRDAQFGPNTGPFLYEFQRHGLPAEE
                     FIGKLDDFFGSVSTDFRYAVEIRNAGLLGPRYHDMLRAHGVAHVYNHWSYMPPLAEQH
                     QRMKTMTAPFTVLRLLTPLKMTYEAAKKRAEPYDKIVGELPEMRKDTVSLIQEATAEN
                     RSTYVLVNNRAEGNAPLTIQALIDRLQTGG"
     gene            complement(8299..9723)
                     /locus_tag="PLS35_01000"
     CDS             complement(8299..9723)
                     /locus_tag="PLS35_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNI17250.1"
                     /translation="MKAGRWVAHTQPRLDHPDQWTREVAAEWVAAVLSMKKGDFCQTT
                     YLRQGQQGIPISPRHKMQLLSALNVFFRDCQEWEWIPRRFDPRRAFAVPRMLLALIHP
                     NPKPLPDDLWAKALSAGLQLGADDLASLSPRWPTRRFYPIEMVRAVAMVWLFSGLRSD
                     EIVRLRVGCATTKVLDEQRGKTVCLLEVPTNKTSRSFVKPVDPIVAAVIDAWEQVRPQ
                     QARFIDRKTGEPVPLLFAFRGRPLAPPYLNHTVIPLICTKAGIPRHDRHGTITSHRAR
                     STMATQLLNSPDHPMTVFELQQWLGHRSVSSTLHYLAITPTRLSESFLKSNHTHRNLR
                     TVQVLIDQDVVLSGDAGTQPWKFYDLGHGYCRYDFFEHCPHRMACAKCSFYLPKGSTE
                     AQLLEGKINLLKMRQEIPLQPAELAAVDEGIEAFERLLTQLRQTPTPAGLTPLQIDDA
                     KTSARESGVQAGLPETSAARPTVT"
     gene            10748..12439
                     /locus_tag="PLS35_01005"
     CDS             10748..12439
                     /locus_tag="PLS35_01005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNI17251.1"
                     /translation="MKREGEDGMEWWNPVARRCLEMTLLALVGGWAYFVNLHLSLLEG
                     SEGLYAGIAGEMGRRREFFDLTYHDVPYFNKPPLFFWLLNLSTSIWGDHEIALRLPGS
                     LAAVGTIILIYVLGVRLVSPAAGFWAALVVATSHVFLWYGRRVLFDSTLTFLVTLALF
                     AWIQVQLLGRSSRWYLLAFVSMALGAMLKEMHGFFLPLLVMALYAAIQRDTRMLKDGY
                     FWAGFALAVALMAGYAQMLGPGYQHHFRISNAIQSVWNTAFTRTSGPATDGHPLYWYL
                     GMMWADFFPWCAVLPSALLLLWAQRPFRAHPTELLLLVWVLSLFTAFSLATLKREPYL
                     TTIVPGIGLMIGYYYHRLFSSAEDSAAMTPLLRVLLGVLAVTFAAGMFFGAAPLRRRW
                     LVSTAVVSPMFIVTIVSLAGLLLYAVLRSRVRLALGMVGALAVAYVVLVVNYVFPAID
                     QAASPRKAVEEIKTLALGTQPALFMYIPGWPKNEDALYYLKRDNVVPDLPSQDAVREA
                     VRTHGAIRVVTEEQHAAALQSQAGLVVGKLQEFRQPGRKHLFLLSVQEQTSARVL"
     gene            12755..13180
                     /locus_tag="PLS35_01010"
     CDS             12755..13180
                     /locus_tag="PLS35_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17252.1"
                     /translation="MHPIHPMVVHFPIALLLASTLFDALAFRWRRHQFRDTSLSLLVL
                     GILAAGAAVLTGHFAEEAVERSGIPKQAIEIHEELGGSVFWVFLGLLGLRLASCWGWM
                     REQPMLVLVIGLSGGLLLLIASYFGGDLVYRFGAGVLPR"
     gene            13231..>13456
                     /locus_tag="PLS35_01015"
     CDS             13231..>13456
                     /locus_tag="PLS35_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17253.1"
                     /translation="MTTRIVMLLILCMLPATAQADASLSRAPWKERAVLRTGQVVQGD
                     YFAFGPHVEISGIVNGDVIVAGAKVILSGTV"
     assembly_gap    13457..13556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13557..13742
                     /locus_tag="PLS35_01020"
     CDS             <13557..13742
                     /locus_tag="PLS35_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17254.1"
                     /translation="VNGDVIVAGAKVILSGTVAQDARVAGAQVVSISLTHRHVVSRER
                     CSSASDMPVIQNHQQDS"
     gene            13971..14210
                     /locus_tag="PLS35_01025"
     CDS             13971..14210
                     /locus_tag="PLS35_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17255.1"
                     /translation="MMSHHETDDRVLEVIVRSQGRTLDDIVLECPELTWNQVFIVIDR
                     LSREGAIRLMPKERGCYTVHLASNVFHDARQYARV"
     gene            14424..14672
                     /locus_tag="PLS35_01030"
     CDS             14424..14672
                     /locus_tag="PLS35_01030"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNI17256.1"
                     /translation="MKAQTTQPFREKKPHTMQVEQPNVPVSTRKAAGSAQAAVDDLHA
                     RITARAYELYVVRGCRNGYHLEDWLDAERVILDRAVTK"
     gene            15325..15927
                     /locus_tag="PLS35_01035"
     CDS             15325..15927
                     /locus_tag="PLS35_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNI17257.1"
                     /translation="MWQIRIHGRGGQGVVTAAELLAMAAFMDGKYAQAFPSFGSERMG
                     APVVAFCRVAEGAIRTRETVTRPDVVIIQDPTLLHQVDLFGGLAPLGFVLINSTQTLD
                     ALHIEEFLSTLPLGHVCAFSATDLGLKYLKRPIANGPLLGAFAAITGLIRLASVESAI
                     QQKFPGAVGEANRAAAREAFDVVTTSITEQRGIVHHAHAD"
     gene            15911..17164
                     /locus_tag="PLS35_01040"
     CDS             15911..17164
                     /locus_tag="PLS35_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNI17258.1"
                     /translation="MRMQIEGSQAVAEAIALCQPEVIAAYPISPQTHIVERLSRKTKQ
                     GHIGRCQFLNVESEFAALSVLIGASASGARTYTATSSQGLLFMAEAVYNVAGLGLPIV
                     MTIANRAIGAPINIWNDHSDSMALRDAGWIQLFAEDNQDAVDLHIQAFRLAESLSCPV
                     MVCMDGYVLTHAYEPVDVPDAVQVNAYLPPYDPVQVLDPERPVSIGAMVGPEAFTEVK
                     YFSHQQHLRALEVMPELVEVFEQHFQRRSGGLCSAYRMEDADIAVIAMGSVIGTIKDA
                     VDELRNTGVRVGCVKIGCYRPFPKFQVSALLEKTTRVIVVEKDLAVGMGGIVSADVQS
                     ALQGRQISVHTVIAGLGGRSISTSSLKHMILEARAERLTEPYFLDLNKTVIEQELARR
                     RDIRRAGPVAEHMLQTMNVIGTKVS"
     gene            17176..18165
                     /locus_tag="PLS35_01045"
     CDS             17176..18165
                     /locus_tag="PLS35_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNI17259.1"
                     /translation="MTTERIKFYQTGTFTAGNRLLDEATRTVQSRLDRTNSLNSGHRA
                     CQGCGEALGARYALDAAMRASHNQLIAVNATGCLEVFSSPYPESAWQIPWMHSLFGNA
                     PAVASGIAAALRAKRRDDVTVIAQGGDGATVDIGFGCLSGMFERNDDVLYLCYDNEAY
                     MNTGIQRSGATPPFARTNTTQAVGEHPGNPIGNGKNVPRIALAHGIPYVATASVADLH
                     DLERKVTKAIGVRGARYIHVHVPCPLGWGSDPAHTMKVARLAVESGLFPLFEAEFGKV
                     ICSTPIRRKVPVERYLELQTRFKHLFGPPQDTEALAAIQAMADDNIAVYGLME"
     gene            18174..19802
                     /locus_tag="PLS35_01050"
     CDS             18174..19802
                     /locus_tag="PLS35_01050"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNI17260.1"
                     /translation="MERTPFAITLDPNTSLANHTGNWRTMKPIYVDRLPPCNHACPAG
                     ENIQAWLYHAEEGAYEEAWQQLMQDNPMPAIHGRVCYHPCESACNRGHIDEAVSIHAV
                     ERFLGDQAIAQGWRVACAPRTGKNVLVIGAGPSGLSTAYHLARMGHQVTIHEAGPQAG
                     GMMRYGIPKYRLPREIVDAEVARIQSMGVTIRLNSRVDDLEATMAEGRFDAAFLALGA
                     HLARRVEIPGVDAKRVLDAVTFLRDMEGAARPKLGRRVAIYGGGNTAIDVARSVERLG
                     MEPVIIIYRRDLGHAPAFASEITEAQDEGVQFQWLRRIAQGDESTLTVEEMALDEQGK
                     PQPTGRFERIEADSVILALGQRADTGFLRRVSGLRVREDGTIEVDGTMMTGRPGIFAG
                     GDMVPGEQSVTVATGQGKKAARHIDAYLRGSAYVKPEAHEPATFSRLNTWYYTDADAS
                     KQPLLNLVRRRSSFDEIVGGLDADTALLEARRCLSCGNCFNCDNCYAVCPDSAVIKLG
                     PGKRYAINYDYCKGCGLCAEECPCGAIDMVREVK"
     gene            19888..20370
                     /locus_tag="PLS35_01055"
     CDS             19888..20370
                     /locus_tag="PLS35_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI17261.1"
                     /translation="MALAQRACSGTALVLCAVLVGPVHAAKVSDAAATHEWMSTPVRT
                     AAEQMLAAMPDDYYSIKGVAAEKAIATGVPHVLDVREPNEFAVEHIPGAKNVPLRSLM
                     TAIDDLPGDKAASLIVYCKTGHRGAIALAVLRMLGYANVLSLYGGLDGWKAAGFAIEK
                     "
     gene            20404..22845
                     /gene="ppsA"
                     /locus_tag="PLS35_01060"
     CDS             20404..22845
                     /gene="ppsA"
                     /locus_tag="PLS35_01060"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNI17262.1"
                     /translation="MSYTATTTQAAIRWFEDIGIDDIPLVGGKNASLGEMYRELASRG
                     VKVPNGFAITAEAYRSFLRTTGLDQRITQIIRGLDTRDLDNLRQRGRQIRQAMLAASL
                     QQDLERAIVEAYDRLSDGHVDLVDVAVRSSATAEDLPDASFAGQQETYLNVQGHQALL
                     DACKRCFASLFTDRAISYRVDKGFDHFKIALSIGVQRMVRSDLSASGIMFSIDTETGF
                     RDAVLINASYGLGENVVQGAVNPDEYYVFKPTLRQGFRPILQKMLGSKEFKLVYDVGG
                     SKLVKNVPVPPDDRARFAITDDEILTLSRWACMIEDHYSAKKGRTSPMDMEWAKDGHT
                     GELFIVQARPETVQSQKRQDSLELYRLGRKGKVLLTGRSVGSKISLGPVRVVKHLQQL
                     RDFTSGDVLVTDKTDPDWEPVMKKASGIITNRGGRTCHAAIVSRELGVPAIVGTGNAT
                     EVLRDCQMVTLSCAEGDTGFVYEEALPFTVQRVELSDLTRPRTKIMMNVGNPEEAFAL
                     SFIPNDGVGLAREEFIISTYIKVHPLALLNYDRLDDAAVKAEIDRLTVGYADKAQFFV
                     DKLAQGVAMIAAAFYPKDVIVRLSDFKSNEYASLVGGRGYEPTEENPMIGFRGASRYY
                     DSRYQAGFALECRAMKKVRGEMGLTNVKLMIPFCRTVEEGRLVQAEMAKHGLTRGENG
                     LEIYVMCEVPSNVVLADQFAEVFDGFSIGSNDLTQLTLGVDRDSEIIAHLFDERNQAV
                     TSMMASVITTARKKGKKIGICGQAPSDYPEVASFLVEQGIDSISLNPDAVLKTTMAIL
                     KLEQGLQRGPAQRTG"
     gene            22868..24070
                     /locus_tag="PLS35_01065"
     CDS             22868..24070
                     /locus_tag="PLS35_01065"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNI17263.1"
                     /translation="MKRIISDLPHDRYRERTVLVRVDFNVPLDHGRIREDYRLRQAIP
                     TIEYLSQRGAKVILASHLGKPKGKVVPALSLKPVADRLGQILRVPTVKFVEAAVGESV
                     KQTLTTLKPGEVLLLENLRFEPGEEANDPVFAKQLAALAELYVNDAFGTMHRAHASTY
                     GAAQLFTDRLAGFLVRKEMDVLSQVRDRPVRPFRVVVGGIKIKDKLSALKALLPKADT
                     ILLGGGIAYTFLAAQGVATGDSPVEPEFIPWAKDVLAQYRKHILLPEDHVIAANAEQR
                     DRLQLVHREIPAGFMGLDIGRDTSLTFAHELLQGTGTIFWNGPLGAFEVDECADGTVD
                     IARAMALAHWRGAFTVVGGGDTVAAVRKAEVLETEISHVSTGGGASLKFIGGEELPGI
                     AVLSEQSQ"
     gene            24089..25147
                     /locus_tag="PLS35_01070"
     CDS             24089..25147
                     /locus_tag="PLS35_01070"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF003251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNI17264.1"
                     /translation="MRKVKVAVNGYGVIGKRVADAVKQQEDMELVGVADVVTDYRIKT
                     AAVLGLDIYAALPDKAQAMRAADIPVAGTLDDLLRKVDVVADCTPKKIGAANKILYEQ
                     AGVRAIYQGGESHALTGHSFVAQANYETAIGRSSTRVVSCNTTSIVRTLGALKKAGLL
                     KRARGTLIRRASDPWEAHLDGIMNTVIPEKTIPSHQGPDAKTVDPDLDVVTIAAKASH
                     NSSHLHTWWVELTRSASKEELLAAFRLAPRIAFIRMHEGVAALNSTIELMSDLGRRRG
                     DLWEVALWEDVLTVNGNEAYYTYQVFNQAIVVPETIDAIRALTGLESQAERSIQKTDQ
                     ALGIAKEFLRSSEHVLMA"
     gene            25282..26346
                     /locus_tag="PLS35_01075"
     CDS             25282..26346
                     /locus_tag="PLS35_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNI17265.1"
                     /translation="MAQTMKAFVMKKLGSVGMMEKPIPDLGPNDAIVKTTAALICTSD
                     VHTVGGAIGERTNLTLGHEAVGVIYKLGSAVKGFREGDRVAVNAITPCYLCENCLRGY
                     TSQCTDMLGGWKFANVKDGSLAEYFHVNSAQANLAPISKGLTDEQAAYCADMMSTGFM
                     GAEHANIPIGGSVAVFAQGPVGLMATVGSRLLGAGLVIAVEAVPERKKLAKEFGADVV
                     IDFTEQDPVEAILGLTGGQGVDSSIEALGAQATFEACIKATRPGGTISNVGYHGEGQY
                     VQMPRKEWGVGMSDKVIRTGLCPGGAERMKRLMRLLETGRVNPLPLTTHRFRFSDVEK
                     AFELMRTKADGMLKPLITFA"
     gene            26598..27173
                     /locus_tag="PLS35_01080"
     CDS             26598..27173
                     /locus_tag="PLS35_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNI17266.1"
                     /translation="MYKTKAYSAASATSSLVSTTIARRDPTEHDVQIEILYCGICHSD
                     LHSVRNDWSSVMSTTYPIVPGHEIVGRVTKVGSAVTKYKPDDLAAVGCLVDSDGTCPN
                     CTAGLENFCPNQTFTFGSPDKHKTAPVTYGGYSDSIVVDERFVLRAPTNLNGAGTAPL
                     LCSALGSPPTHPCATTASPRVRKWASSAPVA"
     gene            27146..27664
                     /locus_tag="PLS35_01085"
     CDS             27146..27664
                     /locus_tag="PLS35_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNI17267.1"
                     /translation="MGIVGPGGLGHMGVKFARVFGAHVVVFTTSPSKAEDALRLGAHE
                     VVVSRDANEMKKHTGSFDFILDTVPVDHDINQYLHLLRLDGNLTLVGVPSQPLALSSF
                     ALIFGRRSVSGSNIGGIPETQEMLDFCGEHNITADVEVIPIQNVNEAYDRLLKSDVKY
                     RFSIDMASLKTE"
     gene            27742..28626
                     /locus_tag="PLS35_01090"
     CDS             27742..28626
                     /locus_tag="PLS35_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016205472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17268.1"
                     /translation="MQTTTLSNGLHMPMLGLGVFQAPPDVTAAAVQEALRVGYRLIDT
                     AAAYANEREVGEGIRRSGLARDDIFIQTKVWISDYGYDATVHAFDKSAGKLGVDQLDL
                     LLLHQPLTSRFDLTLGAYKALEKLLADGKVRAIGVSNFMPDVLQRLLAETSVVPAVNQ
                     IEVHPYFQQKELQRLHAEHGILTQAWSPLGGITSYGGGEKRTFDDPTLQQIAREHGKS
                     AAQVMLRWHLQEGRSVIPKSTKPARIAENLDVFDFELTKEQVASIDALDTGVRSGPEP
                     DSITLENYGREIPEACGK"
     gene            28823..29623
                     /locus_tag="PLS35_01095"
     CDS             28823..29623
                     /locus_tag="PLS35_01095"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI17269.1"
                     /translation="MSKTIFITGASSGLGKATAKLFAVKGWNVLATMRQPAKETELTQ
                     FANVTVLPLDVTRSEQVSETIQSALKSSDVDVVFNNAGYGLAGPMDGIDDARIVRQFE
                     TNVFGAMRVTREFIPYFKQKRSGRFITTTSLAGLIGLPLNSVYNASKWALEGWSEGLS
                     YELAPFGIKVKTVAPGAVMTDFMSRSLDHVPAPGYDAMNEATVKFFVPDMAKISSAES
                     IAQVVWEAATDDQDHLRYVAGDDAIDIYALRQKLGNESFRQHLASLVL"
     gene            29662..30375
                     /locus_tag="PLS35_01100"
     CDS             29662..30375
                     /locus_tag="PLS35_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNI17270.1"
                     /translation="MKPQVICHMLSSVDGRLHVGRYTPLTSGKTSTEASAPYYEYSAK
                     FDADAQIIGGRTARESALPLRYEGDVATRPAHFQTFVGHRQSKRSLIVLDPQGTIRYD
                     SDNVSGENLIAVLGESVSQQYLDHLQAQGVSYLFAGPKGDDVPRLLGTLSADFGMTRV
                     LVEGGGTINGAFLKAGVIDELSLMLYPGIDGLAGIPSIFECQGAPGEKPAEGQSMELV
                     AVETLPDGIVWLRYRFHRQ"
     gene            30456..30740
                     /locus_tag="PLS35_01105"
     CDS             30456..30740
                     /locus_tag="PLS35_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17271.1"
                     /translation="MKYIKLGNTGIDVSKICLGMMSFGKPGSEHGLFPWAVDFEDAKP
                     IFKKAIELGINYFDTANVYQLGTSEEVTGKLIKEFKLDRDDLVVASAAWA"
     gene            30731..31651
                     /locus_tag="PLS35_01110"
     CDS             30731..31651
                     /locus_tag="PLS35_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17272.1"
                     /translation="MGLSFGYGPATDKGDAVKLIRAAFERGVTFFDSAEAYGAVNEEM
                     VGEAVAPFRDKIIIATKFGFKDGNSQAGLDSTPARIRLVAEQSLKRLKTDRIDLFYQH
                     RVDPNVPMEDVAGVVKDLITEGKVKHFGMSEAGVAMIRRAHAVQPLTALQSEYSMFTR
                     EPETEIIPTLEELGIGFVPFSPLGKGFLTGKMDVDTKLDANDFRNTLPRFEAENRRAN
                     LAFVELLTTIANRKQVTPAQIALAWLLAQKSWIVPIPGTTKLHRLEENLGAASVDLTT
                     DDLREIEQGVSKLTVQGARYPEKVEQMTNR"
     gene            31771..33153
                     /locus_tag="PLS35_01115"
     CDS             31771..33153
                     /locus_tag="PLS35_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNI17273.1"
                     /translation="MSYQSVNPFDGTTVKTFEQLTDAQLETALETAANCFENWRRTTF
                     AERGAIAAKAAAIMRARIDEFARPVTLEMGKLIAESRAEVQLSADIIDYYAKTAERFL
                     APEPLQPGSGEAKIESAPFGVLFGVQPWNFPYYQLARFAAPNLMAGNVVMVKHSGTVP
                     QCAIAFEKLWLEADAPAGAYTNLFISYEQVNRVIEDRRVKGVALTGSVDAGKSVAERA
                     GKHLKKTTMELGGSDAFIVLEDADLDKTVKWAVWAKMNNTGQCCIAAKRYIVVEKLAD
                     RFLEKFKSALSALTPGNPMDKATTLGPLSTEGALVNLLKQVDQAVAHGADLVLGGKRM
                     DRPGAFMEPTILTNIKPDNPAYREEFFGPVALMFRVKNEDEAVVLANDSDFGLGGSIF
                     TNDVARGKRVASRINTGMVFINHPTWTAPDLPFGGIMDSGYGRELSSMGIQEFVNKKL
                     VRVESIDAPA"
     gene            33579..34544
                     /locus_tag="PLS35_01120"
     CDS             33579..34544
                     /locus_tag="PLS35_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17274.1"
                     /translation="MEVSALGLGCMGLTSFYNTALPEGEAVAFLRAAYEKGITLFDTA
                     EAYGPFVNETIVGKAVAPFRDKVVIATKFGFDIDLETGRHLGGVNSKPAHIRAVVDAS
                     LKRLATDRIDLLYQHRVDPEVPMEDVAGAVRDLIAAGKVKHFGLSEAGPESIRRAHAV
                     QPVTALESEYSLWHRGPEEKILPLCQELGIGFMPFSPLGAGFLTGKIQADTRFGESDY
                     RNTEPRFAQENREANMALVALVKDAAARKGATPAQVALAWLLAKKPWIVPIPGTTKLH
                     RLEENIGAVDVVLSADDIAEMETTLAGLTLRGERLSAGELAMTGL"
     gene            34552..34992
                     /locus_tag="PLS35_01125"
     CDS             34552..34992
                     /locus_tag="PLS35_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNI17275.1"
                     /translation="MTQTIGIIGSGMIGGQVARLAIAAGFNVVICNSRGPETLAELVS
                     KQGPSARAATFAEVTEAADLIVLAVPFAGYAKLSAEALAGKTVIDTMNYYPERDGVMP
                     EVKTDTIATSELVQKHLSRSRVVRALNNMDFVRLLSRARPKGSA"
     gene            35122..35307
                     /locus_tag="PLS35_01130"
     CDS             35122..35307
                     /locus_tag="PLS35_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17276.1"
                     /translation="MPVYVNPYWDLDDSHAVSSFMVAPGRVVTRQEVAALLGKAIRHD
                     KMFGLLGAFDVRTNRLS"
     gene            35604..36668
                     /locus_tag="PLS35_01135"
     CDS             35604..36668
                     /locus_tag="PLS35_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17277.1"
                     /translation="MKTPLLKLNNGVEMPALGLGVFQSSPEDTIKAVATAIADGYRLI
                     DTARAYKNESQVGEGIRNSGINRAELFVTTKLWLTDYGYDAALRGFDGSLTKLRLDYL
                     DLYLLHWPTKNFDATVQSYKAAEKLLAAGRVRAIGVCNHTEQQLEALIARTDVVPAVN
                     QVELHPYFAQKALRQTSAALGIVMQAWSPIGGVKRYWPGASATGTGDPLADPVITGIG
                     RAHGKTAAQVILRWQFQNGVVAIPKSVKAARIAKNIDIFDFVLSDSEMTKIDALDTGA
                     RGGPDPTQIDMDSFSDTVDRMHPVRVSRRDAKQTEPLNEHRSIRHSCSYHGCRLRNGV
                     GISRGILGSRRVRRAVRKPQ"
     gene            36704..36961
                     /locus_tag="PLS35_01140"
     CDS             36704..36961
                     /locus_tag="PLS35_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HNI17278.1"
                     /translation="MNRVLVITYPKKDDRIASVMAAEYAMTNSWRDQGIIEHLFAKEN
                     DGGGVVVFNETSVEKVQALIAQLPLFPFFEKVEYFPLNKIY"
     gene            37000..37902
                     /locus_tag="PLS35_01145"
     CDS             37000..37902
                     /locus_tag="PLS35_01145"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI17279.1"
                     /translation="MQRVSFKNRNIIMAGSLCQPRDFKDGARYPAVVVVHPAGGVKEQ
                     TAGEYAKRLAAEGFVTLAFDTSYQGESGGEPRYLDEPMNRVGDIYSAVDYLTTLPFVD
                     AERIGILGICAGGGYSVKAASLDRRIKAVATASAANVGNAARKGWDGKSPMSALPGTL
                     EMLVNQRTAEAAGAAIAYAPYVPNVGESSPFRDLREGSDYYLTPRGRHPNAPNKMLMT
                     SLMGWIAFDAFDLVDPLLTQPLMICAGSEADSLWQSQELYAKAPGSSKELFIIERASH
                     MGLYDGSGREAVLGKITPFFRANL"
     gene            37916..38512
                     /locus_tag="PLS35_01150"
     CDS             37916..38512
                     /locus_tag="PLS35_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17280.1"
                     /translation="MSASTPMTSSATSAGQLLARQRRHASPELLAAALLLLGTVAALQ
                     SEYSLWWREPEQELLPTLEELSIGFVPFSPLGKGYLTGKIDETTTFDSTDFRNTVPRF
                     TAENRKANQAMVELLGRIAERKKATPAQVAIAWLLSQKPWIVPIPGTTKLHRLEENVG
                     GAAVELTPDDLRDIADAAANIAVHGERYSEAAQKMINR"
     gene            38626..39744
                     /locus_tag="PLS35_01155"
     CDS             38626..39744
                     /locus_tag="PLS35_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17281.1"
                     /translation="MKYRKLGNTGTVVSTLALGTMYFGDETAENDAFAILDAFLDAGG
                     NLVDTSDVYVGTVAEQIVGRWLAARPREITDRVIVATKGRFGDDSDPNAVGLSRRHLH
                     RALDASLRRLDVETIDLYQLHASDMHTPVEETLSFLDDAVRAGKIHYVGLSNFTGWQL
                     QLMVSTAKATGVQTLVSLQQQYSLLSRECEWEMNPAARHNGLSLLPWSPLAGGFLTGK
                     YQRGGTPASDSRAGSGKDLYQWISADYAGSDQNWATIETVIRIAKEIGATPAQVALSW
                     IANRPGVAAPIFGARNLRQLDENLAAAKLTLDENATKALEVVSAPRSNGYPYGAFGAG
                     QRNRDLRDGNPAPGLPVAGGSDHPLGRPSLTVTSRVSD"
     gene            39769..40215
                     /locus_tag="PLS35_01160"
     CDS             39769..40215
                     /locus_tag="PLS35_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17282.1"
                     /translation="MDRPRIFIHMHMSLDGKIVGSYLPTDVGMASQRQYYNLFLGPDR
                     HFRNHKGWLSGRTTSEDNFTHYRKLKLDEFAPPMPEGDFIAVDDAPMHYFSVDPSGVL
                     AWETNSITYFDTTAHVVQILSGKATNAYKDFLRRKGVSYTSPARTL"
     gene            40239..40502
                     /locus_tag="PLS35_01165"
     CDS             40239..40502
                     /locus_tag="PLS35_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNI17283.1"
                     /translation="MGSAFGVDEIMLCGGGGLNWSFIRADLCDEVSIVLTPAADGTKG
                     AQTLFEADERYTQPLPTAFKLKEVQPLDDGSVWLRYTVEGPIN"
     gene            40672..41760
                     /locus_tag="PLS35_01170"
     CDS             40672..41760
                     /locus_tag="PLS35_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI17284.1"
                     /translation="MCITPALGKPAAGPAPICDQNRTSSPLITQRRTLGSGKHALQVS
                     ALGFGCMGMNYHRGLHPDRDAMIKLARQATERGVTLFDTAEVYGPFVNEELVGEALAP
                     FRGQVVITTKFGWKIDPGSRTSIGLDSRPQHIKEVAEASLKRLKVDAIDLFYQHRVDP
                     DVPIEDVAGAVKELIQEGKVKHFGLSEAGAKTIRRAHAVQPVTAVQSEYHLMWRAPEK
                     DVLPVCGELGIGFVPYSPVNRGFLGGNLNEYTRFDSGSDNRKTLPRFTPEAMRANLAI
                     VEALNRFGRTRGVTSAQVALAWLLAKQPWIVPIPGTTKLAHLEENLRTADLKLSAAEM
                     SEIEGSVSKIEIVGDRYPAEQQKLVSPD"
     gene            41984..42394
                     /locus_tag="PLS35_01175"
     CDS             41984..42394
                     /locus_tag="PLS35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNI17285.1"
                     /translation="MNKPEFFVTPGYGERNLKLFHYSQAVKIGNRLELSGQGGWNDDW
                     EFPESITDEIAQAFRNVERTLAVAGARWEYVIHVNSYHVGLDGDQEEINQAMTERFRH
                     YMPGHAPVWTCLGVAALGDPKMRVEIRVTAILPE"
     gene            42533..43549
                     /locus_tag="PLS35_01180"
     CDS             42533..43549
                     /locus_tag="PLS35_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNI17286.1"
                     /translation="MRGAVLYGPRDVRFEERDAPKIIEPTDAIIRIALTCVCGSDLWP
                     YRGLQPITEPTPMGHEYCGIVEEIGGAVRSVKPGQFVIGSFATSDNTCPHCQAGYQSS
                     CVHREWMLRAQAPVLRVPLADGTLVPTPGVPSNDLLPSLLTASDVLGTGWFAADAANV
                     TPGSTVVVVGDGAVGLLGVLSAKQMGAERIIAMSRHEKRQQLAREFGATDIVTERGDE
                     GTTQIKELTNGVGADSVLECVGTQESMMQAILATRPGGSVGYVGVPHGVLLDGEKLFY
                     SHVHLHGGPAPVRRYLPKLIDLVLDRKINPGKVFDLTLPLDQVAEGYRAMDERRAIKA
                     LLRP"
     gene            43610..43885
                     /locus_tag="PLS35_01185"
     CDS             43610..43885
                     /locus_tag="PLS35_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17287.1"
                     /translation="MPRPSRPGATLEKQQPHLHDEARHIWESYKKGVIRDISFRQDRL
                     GVATTLERNCAGSLKPMPCTFRNDVEKAFELMRTKADGMLKPLITSA"
     gene            44054..46474
                     /locus_tag="PLS35_01190"
     CDS             44054..46474
                     /locus_tag="PLS35_01190"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNI17288.1"
                     /translation="MHTKTFFCIISVIFLLSWRSNVEAKSLPVVQVGIVRDGPSELVP
                     ELRDLIQKEILEITRGEFEVRFPNDLQFDGGWSVQGVKQAIDRVLAQPRVNLVIALGI
                     LASQEVSQRRQLPKPVIAPLILDPHLQGLPFKKGTSGMKNLSYLVTGKGFERDLEAFK
                     EVFPFTRLTLLADRVLLEAFPPVKDRARKMAETYGVKLTVVPVETSAEAAIGSIPPDT
                     EAVYLSPLPRLLAGEFDRLVLALNRRKLPSFSAVGQRDVQRGITLALSPELDLGRFAR
                     TIAANVQRILSGTDAGRLEVAFIRGEQLVINMATVRAIDKWPSFQVLSEAELVNEDAA
                     GTSRRLSLFEAVRESAEANLDLAAASRKVAAGVGQVGQARSGLLPQVLVGTSGVLNGR
                     GPDTFGTGGTPGQAAIALGGFRQLLYSDDAWTKYTVQQKTQLSREEEWNQLRLDIAQD
                     AALAYLTVLRTKTVRRIQKDNLKLTRANLDMAAARESVGYALRDELFRWEAEVANGQK
                     AVVSAEAQERQAEVDLNRILNRPLEERFQTVEQTLEDPGLLGDTRQLFAYIENPRGFQ
                     VFRDFEVEEGIQAAPELRRLDALSAAQERTITNAQRAYWLPEVAIQGAGFQQYAQGGG
                     GAGSLTVPLGPVGFAQTQNNFYVASIGLTFPLFQGGYKDATALKAREELRQVQFERAA
                     VALQVEERVRTTLYQTGASFPSIRLSRKAAESARKTLDLVVDQYSRGAVDIIKLLNSQ
                     NAALTANQAAANAVYEFLIDLAKVQRAVGQMSFFRSSEERAAWFERLKTHFVNAGVSP
                     VLRDDPRN"
     gene            46493..47485
                     /locus_tag="PLS35_01195"
     CDS             46493..47485
                     /locus_tag="PLS35_01195"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNI17289.1"
                     /translation="MPTPKIARPTLILGVGLLLLVAGASVWWFYFRIPPLVGFGSGNG
                     RLEVQEIDVATKFPGRIAEVLVDEGDSVQVGQVVARMDTSSLNAQVREAEAQVQRAKQ
                     GKITAKAVIAQRRSETLLAERDLERARALYVNANISVKDYDRARSTMDTAKSAITQAE
                     AQLAEAEAAIKASLAQSERIQADLKESVLTAPRSGRVQFRLAEPGEVLAAGGKVLTLI
                     DPTDVYMTVFLPAAEAGKIALGAQARITLDAAPNLVIPAAVSFVADKAQFTPKEVETR
                     SEREKLMFRIKVKIDPELVKGHEAQVKPGLPGVAYVQLDSSVQWPPSLQTKLIR"
     gene            47482..>50057
                     /gene="rbbA"
                     /locus_tag="PLS35_01200"
     CDS             47482..>50057
                     /gene="rbbA"
                     /locus_tag="PLS35_01200"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HNI17290.1"
                     /translation="MSEPSASVARVATLTHRYGDTTALDTLTLDIPAGRMVGFIGPDG
                     VGKSSLLGLIAGAKKIQAGRVEVLGGDMAAARHRNEVYPRIAYMPQGLGGNLYLTLSV
                     FENVDFFGRLFGQNREEREWRITDLLRSTGLTPFRDRPAGKLSGGMKQKLGLCCSLIH
                     DPDLLILDEPTTGVDPLSRRQFWDLVDRIRKRRAGMSVLVATAYMEEAERFDWLVAMD
                     AGKVLATGSPAELKGRMNVRTLEDAFVSLLPEEKRRGHSAIAIPPRRTHEGPPAIEAH
                     GLTMRFGSFTAVDHVSFRIERGEIFGFLGSNGCGKTTTMKMLTGLLPASEGDARLFDR
                     PVNAQDMETRKRVGFMSQAFSLYGELTVRQNLELHARLFHLQPEKIPGRVAELTKRFK
                     LEDYLDDLAEALPLGIRQRLSLAVAVVHQPEILILDEPTSGVDPVARDGFWELLIELS
                     RKDRVTIFISTHFMNEGERCDRISLMHAGRVLVSDTPEGLIRTRGAANLEEAFIGYLE
                     EASGEATQKEVVEEAAETMPSAAGTSRADRGRAFSPMRLFAYAHREALELRRDPIRLT
                     FALLGSVLLMFILGYGITMDVEDLRFAALDRDRTPQSRDYIQNLAGSRYFIERPFITD
                     DAELDRRMRSAELSVAIEIPSGFGRDLKRGRQTEVGVWVDGASPFRGETIKGYVQGMH
                     YQYLADLVRRTYGAQPQAGLADLEMRYRYNQDFKSLESMVPAVIPLLLVFIPALMTAL
                     GVVREKELGSITNLYVTPVTRLEFLLGKQLPYIALSTISFFGLVALAVVAFKVPLKGS
                     FATLTLGGVVYVIVTTGIGLLMSAFTRSQIGAVAGTAILTMLPTVQFSGLTDPVSSLE
                     GIG"
     assembly_gap    50058..50157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <50158..51131
                     /locus_tag="PLS35_01205"
     CDS             <50158..51131
                     /locus_tag="PLS35_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17291.1"
                     /translation="IGIVDEDQSPLSARIISAFYGPYFKPPRVISMDEIDRGLDAGEY
                     TFVLDIPPDFQRDVLAGKQPSIQVNIDATRMTQAFIGANYIQSIVTGEITEFAQRHRT
                     GTVLPITLATRVKFNPNLNGIWFGGVMEIISQVTMLSIILTGAALIREREHGTLEHLL
                     VMPLIPFEVMAAKVWANGLVVLVCTALSLRLVVQGALGVPIVGSTPLFLFGTMLHLFS
                     TTSMGILMATVARSMPQFGLLMIIVVLPLQMLSGGITPRESMPEIVQNIMLAAPTTHF
                     TALAQAILYRGAGVDVVWPQLIAIMGIGTVFFVGALLRFRASLAAQS"
     gene            51268..52185
                     /locus_tag="PLS35_01210"
     CDS             51268..52185
                     /locus_tag="PLS35_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HNI17292.1"
                     /translation="MRQGRWAVGILALAVATAGPVLAEEPPAGQAVDCRYEYATEGVT
                     GAAFPTDDVFRPLMADPKQPQFFASYLAARARTDGTHANIGSVGFGENFGFYTRREGC
                     NGWQVGLLAGVFSQFDLSAPSSDLINTDYVVGVPLSWRSGGWSTRVRLYHQSSHLGDE
                     FLLGRPGFNRLNLSFEELEALVSYDYRWGRLYAGGGYLVHREPASLDRNRVQWGVELR
                     GPTIPSPILGRALAGFLVTPVLGADFKSFEELNWVINANVVGGIEWSRQGSSRRFRFL
                     VNYYHGFNPYGQFFAQKVETVGFGFYLQL"
     gene            52272..52616
                     /locus_tag="PLS35_01215"
     CDS             52272..52616
                     /locus_tag="PLS35_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI17293.1"
                     /translation="MNALARALFILLILIGAVQGCGLWAAEGGASKGKRLFEEHCMVC
                     HGQGGKGDGYRLFNPPPADLTSPAVQEKLDAALLRTIHEGRRNTAMGRWKYVLSDEEA
                     REVLAYLRSLSQ"
     gene            52653..55028
                     /locus_tag="PLS35_01220"
     CDS             52653..55028
                     /locus_tag="PLS35_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNI17294.1"
                     /translation="MSRASQSPLSPEMLRKIDAYWRAANYLSVGQIYLYDNPLLKQPL
                     KRTHIKPRLLGHWGTTPGLNLLYVHLNRLIKEQDLNVIYIAGPGHGGPGLVANAYLEG
                     TYSEVYPNISQDEDGMKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYSLSHAYGAA
                     FDNPDLIVAAVVGDGEAETGPLATSWHSNKFLNPVTDGVVLPILHLNGYKIANPTVLA
                     RISHDELDHLFRGYGYTPYFVEGRDPRTMHQLMAAALDAVLEDIRRIKARARRGGAHA
                     RPLWPMIVLRTPKGWTGPKQIDGKRTEDYWRSHQVPMGDMDKPSHIKILEKWMKSYKP
                     KELFDKTGRLKPELSELAPKGTRRMSANPHANGGLLLRDLCLPDFRDYAVKVTKPGAI
                     VAESTRLMGKFLRDTMKLNMEGRNFRLFSPDENNSNRWQDVLEVTNRTWMADRYPYDD
                     HLAPDGRVMEILSEHQCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLKVCN
                     HIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVVRVYLPPDANCLL
                     SVTDHCLRSRNYVNVVVAGKQPAPQWLAMDAAVKHCSAGLGIWEWASNDRGSEPDVVM
                     ACCGDVPTLETLAATDLLRRHLPQLKIRVINVVNLMKLQPPKEHPHGLSDKEFDALFT
                     TDKPIIFAFHGYPWLIHRLTYRRTNHPHLHVRGYKEEGTTTTPFDMCVMNDLDRFHLV
                     GDVIDRLPQLGSRAAYAKQAIREKLLEHKQYIATHGDDMPEVRDWKWSRNG"
     assembly_gap    55060..55159
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <55160..>56225
                     /locus_tag="PLS35_01225"
     CDS             <55160..>56225
                     /locus_tag="PLS35_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNI17295.1"
                     /translation="DWEGDIPLKRPFAKTVIYELHVRGFTRHPSSGLSHETRGTYRGL
                     IDKIPYLQDLGVTAVELLPLFQFDEQDAPRGLVNYWGYSPISFFVPHHGYSSRKDHLG
                     PMDEFRDMVKALHRAGIEIILDVVFNHTAEGDRAGPTLCYRGLENAAYYMLEEDRSRY
                     ADYTGTGNTLNANQPIVRRLILDSLRYWVESMHVDGFRFDLASILSRDEDGRPLKNPP
                     ILWDIESDPCLAGTKLIAEAWDAAGLYQVGSFIGDSWKEWNGQFRDDVRSFMKGDNGT
                     VSQVARRLLGSPDLYGHEEREPEQSLNFVTCHDGFTLNDLVSYNHKHNEANGEDNRDG
                     TDFNLSWNCGVEGPTDDPSIE"
BASE COUNT        11665 a        15638 c        17200 g        11422 t
ORIGIN      
        1 ccgccatgtg cgaggcaatg atgctattga tcgtgagcga ttgcgggtca cctagggcaa
       61 gttccgcaca tgaccgctgt tgagtattga cggggcggcg ttctcgcttg agcaactctg
      121 ggtgcacaag cgctggcgcg gggagtgact ggcacacagt gcgtagctga aagctctcgt
      181 gtgggttaaa gtctgtgacg acgttgccaa ctaacacttg cccgtgggtg gtgaggttcc
      241 cgccatcaat cagccatgcc tgggcatcgt ttctggtgtt ccatcgcgcg agcgcgtgat
      301 gaaggctgcg cctggctctt gctgtgtcaa cgcaaccgag aagaatggtc agggtgtcgt
      361 actcaggatg ggcctctttc cccgagaaat gccgcggaca cgcgcgaatg ggaatccccc
      421 aagcggtgga gagtcgaaat gctaaggcct ctgctttatt gtgtccgatt tctgcctgac
      481 agaagttttg acgacgtagg ttcttctctt caattgtgtc cccatcatag aaagtgacca
      541 tcaccgtatc aaacagacga aaacactcgc gtgcgaggcg tgcgacgtgg ataccgagaa
      601 acgctccgat gcccccacat ccgatttgga tgacatggag gcgtgggcac tgcttaggaa
      661 gcactttcac ggcgtgcaca tgactaagat ctaggtgtgt cctggtgctt ttttctgtgc
      721 tgcgcttccc tgtagcgaca acacgtcgcg gcgacatctc gtccctcatg gtgtgtacgt
      781 gtcctgaagt gtgggaggga gctctgcaac gaactccgct gggattggta tgaaatggcc
      841 atacagcccg acacgaaacg taatggacgg gcgagtggtc aaccatccac acacaccgta
      901 tattcgaaac cctgtttcgt cttcattgtc cacggcggaa aaccgtggag ccatggtgtg
      961 gtgtgagtga atttcgacaa tggcacgctc gtaagatgga cagcgttcag gggtggtagg
     1021 ttggactgag aggggcgcac gaatctggtc tggaatagtc atgtgccaat ctgtgtcgtg
     1081 ttcaaaatgg aatagacttt ccaggggacc cgttggctct gcgcaggctc ccagtgcctc
     1141 ttcaatcatc tcttggatga gccgttgtgg aattctcgga atcgtgagtg tgaggctgct
     1201 tgcaatgggt ggaaggccgg caattggatg ttcgacaact ggaaaaagca ctgacagctc
     1261 tctacgtttg ccgcgaataa acaccccgtt gccagccaga atatagtcgt aaagggtgga
     1321 attggaggcc ggaagcggac cactcgcaat gtggtggttt atgaagggag acgccgtcgt
     1381 catatgtgat tacccttttc cgctctgcat gagttgccga atgatggtgg caattgagag
     1441 gtgagccggc tcaagatcat cgagaggata gtcttgtgcc tcagttgagt gaagtttcgc
     1501 caggaaaggt aggatattgt ccgggtgggc gttggatttc ccattcgcga gatggtcgga
     1561 aaagttacta tcccagaata gtcgccagct gtttgtaatc gtgtttccgt gggcgacccg
     1621 tggatggacg tttccgaaac agatcgcccc atcagggtac acgttagcga gcggtgcttt
     1681 gaagagcttc gttgccgcgc acttccatag cttgtaggcc cagacgtaat agtgggtccc
     1741 gtacccgaga aacagcaacc ctggcatcgg gacggcaagc attgtgtgtg gttctccgcc
     1801 tccggaaagt gtaatggggt cagccaatgg gatttggtat cgaccagggg caaagcggag
     1861 tagtagccac gggcctctgg ggcctatgcc atagcggcag acatgttctg ggagccagtc
     1921 ggaatctatc ccggctgaag agaacgcatt cctggcagcc tctggtgaaa tacattttcg
     1981 gataaccgct tcaccatctt tgtattcaag gagcaattgc ccgccgatga agtgcagtga
     2041 cgcgtcgtgc tgatccgagc tcgcacctct catgtttgcg cctcatgtgg tgcagtgcaa
     2101 agatctgacc agcacctgaa aatcgtttcc catgctgacg gattgtcgag taaggcttta
     2161 gtcgtgctat gcatggattg ttgcatgatt tgcgcccgtt tccattggcg agccaaagct
     2221 ttgagatccc gtttattcca ttggtacggg tgatctgggg actcgtagtc gtagctgatg
     2281 tcgaggaaca gagatccggt gcagtggccg aggatgtcga tgacctgggg aaatcctgaa
     2341 atgggaaagc tgcgtcgcgc acagcgtctt ctgaagtcaa gaaattggac gttgcatcgg
     2401 tgttgaggat tcaaaaagca tcgtggaatt gtagtgtgct cgctgagttc ttcctgaatg
     2461 ttcgtccagg tgggagcgta ggaatagtgt cccacccagg ccgctgcgat acagattgga
     2521 agcggtgcct cctcgaattc ggattcacaa tcataatttt ccagatgtat gggaatgaat
     2581 gggaagcggt ctcgaatcca ttcggtgtcc cagctatcga gcgggaagta gtgttgctcg
     2641 attagcttaa tgaactcttc ttgaagttgt tctgcggcct ccttgatgct tccatgcgga
     2701 agggtggcgg cggtcgtgcg aaacctccgg ggaaagatct cgtggaacgc gttgacgata
     2761 gaaaagtctc gctctcgttc ctccaggtat tcgaggcatg aggggaccga gataacctct
     2821 atgtttttga gggatgcgag aacgaggctt aatggcgcgg agggcgggtt agaacccagg
     2881 tggaaggagg cggctgggcg agccaggagt gttcctgagt cgctccttaa tggtagcagt
     2941 ctctttgtat tccttttcac cgtgctcaat ggcgagacgc agcttttgct gcgtgaggag
     3001 tatggattcg accgtcgtct gggttggctg ggactcggct tggtatgcct ggtagagcgt
     3061 gagggcggga ttgacgtggc ggggggactc gataagctga cggaggatga catggtaatt
     3121 tcctttcgtt ccagcctgtt tcgtgagcct aatcgttgtc atcccattcg tgtcagtggt
     3181 tcgctgaatg gtggcatggg cggcttgcgg gaagaggggg gcaatcgctt tgcgaaccgc
     3241 atcgtcgctg atggcgattt cggggggcaa ttgaatttct tgtccttcaa gttgaagcgt
     3301 aaccataagg ctcctttcgc tactcgaata agccggcttg agggagggct gtgtttgagg
     3361 tggaatcgtg tgtgtgttgt tcacgatccg gtttgggtgt tagggccacg ggcggcgcct
     3421 tcgcggcggc cggcttagca actttgggtt ttctggcttg ccgttccgct tcgcgtttct
     3481 tctcctgttt cactgtcttg gcctgttgct ccgcggcttt tgcgtcccgc tgggttttcc
     3541 gtgccgttaa cgctgcagta aaggtctgct cccaacttgc gagggcctgg ctcagagatt
     3601 ccggaagggg cagtaagtcg ttgaatcgtg cggtagtcag ctgcggcggg aggttgtgac
     3661 tccgtacgcc gatgagtgtg aggcggccgt cagggtggtg gtccgttggg agcagcgtca
     3721 tcacgatgga gatcgtagct cgctgaaaat cgttggagtc ctgttcgaag ctggcaattg
     3781 gatcctgttc gtcttcgtcg tcttcttctt ccagccgtgg gggcggagga gcgggcaccg
     3841 cctcggtctt cataggaact ggctcggttg ctagctgggg catgactgag gaaacgtccc
     3901 cactctgagg cgggtcagca gaggaagaat tggcaggtat gttgttcgta accacttggg
     3961 cgtgtgggag caaatcgaca gtgccaagca tggaggtcct cattatggtg tgcacaatgc
     4021 aggggggatc gtgaggcggt ggttattgtc gtggtaggcg ctggctcaag gactggagtg
     4081 tgttggggcg aagctcttgg ttgaggatgt cggagtctat ttgcaagagc tgcgtaagtc
     4141 aggcgatcag ggcatgcatg gattgtacaa aatggagtgt ggtccgatgg atgggcgcgg
     4201 catgctgtag acggtgaata aactcttgca tgttgatggg atgtgcgagg gcttgtgcta
     4261 acggcgcgct tcttgtcgta ctggtagaag agccctcggc acctgaacgc cctgcgctca
     4321 atactggagt cagcacaata gtcccggctt ctctcgaaag gcgatgcgca agtttgtcgt
     4381 aaaactcggc tcggtctcgg cgccattgcc ggcgccgtct cactacatcg attcgtgtgt
     4441 ctcggatttg cgagagtatt tctcggatta tgcttgtgag gggaccgctg taggcctgtt
     4501 taactgcctg atgagcaatc catcgaagcc tctttctgct cagtggcacg tcgctgtgaa
     4561 tgggctcctt gaccatatcg gcgagtgccc cagcaaatgg aaaatgaaca aatagttgtt
     4621 tgagggcgag gtgctgaaca ttaagcgctt ccctggcttc gtatgccgcg ataagattct
     4681 ttaccacccg tgcgggaaag aagacggatt cgtgtttgcc cgtgtcagat acccattctc
     4741 ctaccctaac aagcgggtta gcgtcattta tgagtcgatg atcaaaatca agggcgatca
     4801 tcccgtattt tttgggttcc gattgctggg tcggcggtac gattagcgtg aattgaaggt
     4861 accactgcca tgtcgtgtcg ttcctgagac atgcgttgga actgtccatg ttgacgctcg
     4921 tctgtggtcg ctgttgtgag ccaatgagag aaacctgttt gagaagagcg tcttttggca
     4981 gaagcggagt aaggatgatt tcaaacggaa ttgggacgtt gcgaacgaaa agctccccct
     5041 cagttctgca aaaatctcgt tgcccccgtt ctcggggatc ggtgtcctct gcgaattgcg
     5101 acaccgggtg gagtgtaagc aggcgattgg cggcaaatga ggagcccgac tgggtcacgt
     5161 ctgcgcgagt caacgattgg attcgaattc cttgcgaacc gaagtcgtgg gtaaagaata
     5221 acgaggtagg tggacctgtt tccttgctgg gtgggagatt tccgacgggc acgcggcgga
     5281 tggcggtttt cattcgctcg agcacggcat tgctgtgtga acgagacaga gggcttgtgc
     5341 gtgcgagttg atgcagtgtg tcatgcagac gtttggtgat ctgggaaagc tcctgcttca
     5401 tgctctcagg gtaagggaga catcggtcga tattgtggtg ttcggaggct tcgtactcag
     5461 ccgcagctgt gcgataggtt cgagaaaggg caagaagagt ctcgtcctcg agggagtcgt
     5521 tttggtcacg caattgtttg atgcactcaa agggatcgcc aattgaacgt gtgccgccag
     5581 ctgcgcgcgt cgttgtacga ctcgtttgaa ggaattgttt gtgaaggcgc tcctgttggc
     5641 gatgggcgac ttcataaacg tcgaccatcg cattccacag cgattgcatt tgatcagctt
     5701 cttctctgac cgcagccggc aaggcagcat aggaagaaaa tgtgagatat gcgggatacg
     5761 tatagacccg tgttagcgat gtcctaatgc cggcgggcat ggtgcaatct cctcttcaag
     5821 actctcgggt gtcagtgcga cgcattgctg tgcttcgcgc atgatcggtt tcaagtgact
     5881 gtaggcgaca gtgcccaagg agattttaag tggtttcccc atctgagcgt aggagcattt
     5941 gccttatgtg attggggttc cccctaacgg aacggcaatg gccgtatcgg agtaggtgat
     6001 ctttggaagg gcgaagtaga ctcccggggt gcgcttttca aagagatagg atggaagtcc
     6061 gattgtgcat cgtggggttt gtgtcggctt tttagctggt tgtgttggtg tatggaatgt
     6121 ccttcactcc tcgacgtgtc tatggccagg gtctttgcag cattgaagtg ttgatcaaga
     6181 gatgcctgaa tccatgaggt tgcgattggt gatatagagc attccctttt gcgcccgatt
     6241 attcttacat caactcacgc aggtaatact caaaaggtgg tttcaataga acatgtcata
     6301 aggcgatccc atgcgatggg ctgaccaata caacgttatg cggtcgatgt tattgtcttc
     6361 ccccgcaact cattggtggg ttcgtgatct gactctccgc ctagacgcca aagacccggt
     6421 tgatgtgttg aatggactcg aggttgtggc aagactgatg cggatgcgtc atgaggccgt
     6481 tcgcgcagga gaatgggatc agttggagtg tcagaaaccg accggggaag acgggatatc
     6541 aatgaaagac tgagacaaaa actcctagtc gtgaagatat gatctcaggt gaccagggct
     6601 tttagatcgt gatacacggg cgagtggttc ttggtcccgt cgaaagagga atatgacaaa
     6661 gtcgatagcg tgagtacact ttgatctatg tccattgccc aactgccaaa tagcgttttc
     6721 gaggacgcgc ctctgtcctg tgtgttccat tcagcacaag acaatctcta cgacgtgggc
     6781 aagattgttg ggctctcaat gcaccactgc catgtcgaga gtcaggccga agtgacgccg
     6841 gggatgacgc tgtccttatt cgttgtcctg ccgacaaagc gtcgggctct cgtgttggat
     6901 gaggctttgg tgacgtgggt acggacggaa gaattcggag tacgtttgaa aacgattcca
     6961 ggtgaagatg ccgcttactt ggagtcctac ctcgccgggc ataaggtgga cgctggcttg
     7021 tgttgaatat tcatcgcatt gatcacgagg cctcccacac gcactgttgg cgggtgactg
     7081 tacaacgtcg tacccgcatt tacgtgcgaa gcttcagtga cggccgctac ggcggagctg
     7141 agcaggcgtt ggttgcagcc acgacctacc gggacgcact gatcaggtcc aatcctccgc
     7201 tgagcaagcc agtctactgt gccattctca agaagacgaa tcgctctggc atttcagggc
     7261 tgagctgtat cgaccgatgg gaattctcca aggggcgccg cgtccatcgg ctctactggg
     7321 acgtccaatg gccgatcgag aactgtcaat cccggcacaa gaagttctcc atcctgaagt
     7381 atggagaaga tggggcgtat cgacgggccc tcaaggccag ggcggccgcc ctcaaggcga
     7441 tctcgtcaca gacgttttcc ccattcgcgt cgagagtcgc tgcaaacagt gcagctgcat
     7501 aggagttgtg ggtccagatt gcgtttcagc ctccggtttg taggcggtcg ataagtgcct
     7561 gaatcgtcag tggggcattc ccttcggctc ggttgttgac gaggacatag gtcgaccgat
     7621 tctctgctgt agcctcctgt ataagactca ctgtatcttt gcgcatctct ggtagctccc
     7681 ccacgatttt gtcatagggt tccgctcgtt tcttcgctgc ctcgtatgtc atttttaatg
     7741 gggtcaagag acgaaggacg gtgaacgggg ccgtcatggt cttcatgcgt tgatgctgtt
     7801 cggccagcgg gggcatataa gaccaatgat tgtagacatg agcgactccg tgcgccctca
     7861 gcatgtcgtg gtagcggggc ccgagcaggc cagcattccg tatctccacg gcgtagcgga
     7921 agtccgttga gacgctgccg aagaagtcat cgagtttccc gatgaattct tccgccggca
     7981 gcccgtgccg ttggaactcg tagagaaaag ggccggtgtt tggaccgaac tgcgcgtctc
     8041 gatagggctg cagcacgagt ttggtgaagg cgtcggcatt gcgaaagttg gggttcaccg
     8101 gcccagcgcg cgagctttag cgagcatgtt tcgcataagt gggaattatg tggaattagt
     8161 gttcaaccga gcagttggct cgcatgccat gtcatgcaaa ccccaccgaa cgagtgctgc
     8221 tcaggaagaa gggggcacct ggcctcaccc gatagggcca tctttgggat tacgctggtg
     8281 ctgagctgat gacagtgatt aggtgaccgt cggtcgtgca gcagatgttt cgggtagccc
     8341 agcctgcacg ccactttccc tcgccgaggt tttcgcgtca tcgatttgaa gaggggtcag
     8401 accagcggga gtgggtgtct gccttagctg agtgaggagc cgttcgaacg cctcgatccc
     8461 ttcatccacg gccgcaagtt ccgccggttg cagcgggatc tcttggcgca ttttcagtaa
     8521 attgattttc ccctcaagca gctgcgcctc cgttgaacct ttgggaagat agaacgaaca
     8581 tttggcacag gccatccggt ggggacaatg ttcaaaaaag tcgtaccggc aatatccatg
     8641 accgagatcg tagaatttcc atggctgggt gcccgcgtcc ccgctcaaga ccacgtcttg
     8701 gtcgatcagc acttgtaccg ttcgtagatt ccgatgtgta tgattcgatt tcaggaatga
     8761 ctcagacaag cgtgtcggcg tgatagccag atagtgcaag gtcgaactca cactccggtg
     8821 gccgagccat tgctgaagtt caaacaccgt catcgggtgg tcaggactat tcaggagttg
     8881 cgtggccatg gtggagcggg cgcggtgact cgtgatcgtc ccatgtcgat catggcgagg
     8941 gatcccggcc ttcgtgcaga tgagtggaat gaccgtgtga tttaggtagg gtggggccaa
     9001 tggtcttcct cgaaaggcaa acaagagcgg cactggttct cccgtcttgc ggtcgatgaa
     9061 ccgcgcctgc tgtgggcgaa cctgttccca ggcgtcgatg acggcagcca cgatgggatc
     9121 gactggtttc acgaaggacc gggaggtctt attcgtcggc acctcgagca gacacacggt
     9181 tttcccccgc tgctcgtcca acactttcgt ggtggcgcac ccgactcgga ggcgtacgat
     9241 ctcatcgctg cgcagcccgg aaaataacca gaccatcgcg accgcgcgga ccatctcaat
     9301 cgggtagaat ctgcgtgtgg gccaccgtgg cgagagggag gctagatcat cggctcccag
     9361 ctgtaatccc gctgacagcg ccttcgccca cagatcgtcg gggagaggtt ttggattcgg
     9421 gtgaatgaga gccagcagca tgcgcggaac ggcaaacgcc cgtcggggat caaatcgacg
     9481 ggggatccat tcccactcct ggcagtcgcg gaagaagacg ttcaaggcgc tcaggagttg
     9541 cattttatgt cgcggtgaaa tggggatgcc ttgctgtcct tgccgcaggt aggtggtctg
     9601 gcagaagtcc cccttcttca tgctgagcac cgcggccacc cattccgccg ccacctcccg
     9661 cgtccactgg tcgggatgat cgaggcgtgg ttgcgtgtgc gctacccagc ggcctgcttt
     9721 catgaggacg gctctcccgt ttcgtcggct cagaaattgg cgggtggagg tgtggaaccg
     9781 tcggttcacc gcgcacgccc atctcggagg cacgcctgtt tcccctacct gggcttctag
     9841 gagcctgtcc gacattgccg aaattgactg atatgggagg atgaaatcat attgatcgaa
     9901 ttgaaatcgt tgatcctgag ccgaatgtgt gcacacgaaa ctttcgtgtt tcctaatgtc
     9961 ggacaggctc ccagcaggct gcggaaaaac tcgattgttg cgtcagacgc cgcgattagc
    10021 tcacgtgccg tgtcggtgtc agaatcgtcc gcaggatgcg caaaaaggcc gtccagcaag
    10081 gccgcagcgt cacgtctgtg gacgggcgcg agtcggtgag cgcccggtgt cccggaggcg
    10141 aatcgttcat tcgtacccgc ccaccccgag cctgccaaga caagcccttt tcccgtggcc
    10201 gtacgttgag cgtttgaggt tcacgacgcg ctgaataaag cgcaccacgc ttgtgaacgc
    10261 cgtcgagata gtgaggcggc agtgtcttgc gagaatgccg ctggcggact ttttccgcat
    10321 cctgctagga aagtcaggtg ccggactgga ggcgggtgat cgacgagctt cccgagcctg
    10381 ggaaagcttt tcgaaacggg accagaaccg gttcgtcggt cgcttggctg ccaagtgagg
    10441 gctcgatcaa ttgtggtctt gttgcgtgaa gccgtatgat agagcgcagg ggcatgcggg
    10501 tgtaaggggc tgatggtaaa agggttggtc ggggaccatg agcatagttg cgtcggaacg
    10561 ccaaaacccc atttgcaagc tgtccaacat gatcgatctc gttgcccgcc atgtttgaac
    10621 cattaattgg ccgttgatag tgcgtgtgtt tgggggctcc atcacacact tcgtggatgt
    10681 cgatgcgaaa tctgccgata tgttgaatat gctgcgaagc gtcgaatgag tgttcatagg
    10741 aggaggaatg aaacgggaag gggaagatgg gatggagtgg tggaatccgg tggcgcgtcg
    10801 atgtcttgag atgacgctgc ttgcgttggt cggcggttgg gcctattttg tcaatctcca
    10861 tctgtcgtta ttggagggga gcgaaggact gtatgccggc attgcgggag aaatggggcg
    10921 ccggcgtgaa tttttcgacc tgacctatca cgatgtaccg tacttcaata agccgccgct
    10981 gtttttttgg ctgctcaatc tctcgacatc gatatggggc gaccacgaaa ttgccttgcg
    11041 cctgcccggc tcgctggccg ccgtgggcac catcatcctg atctatgtgc ttggagtcag
    11101 gctggtgtct ccggcggccg gattctgggc ggcgctggtg gtcgccacca gccatgtgtt
    11161 cctctggtac gggcgccgtg tgctgttcga ttcgaccctc acatttctgg ttaccctcgc
    11221 cctcttcgcg tggattcagg tgcaactgct ggggcgaagc tcacgctggt atctgctggc
    11281 cttcgtgagc atggccctcg gtgcgatgtt gaaagagatg cacgggtttt ttctcccgtt
    11341 gctcgtgatg gcgttgtatg ccgccattca gcgcgatacg cgtatgctga aagatggata
    11401 cttctgggcc gggttcgcgc tggctgtcgc gctgatggcg ggatacgcgc agatgctggg
    11461 tcccgggtac cagcaccatt tcagaatcag taatgcgatc cagtcggtct ggaatactgc
    11521 gttcacccgc acgtccggcc cggctaccga cgggcatccc ctgtattggt atctggggat
    11581 gatgtgggcc gactttttcc cctggtgcgc ggtgcttccg tcggcgctcc tgttgctctg
    11641 ggcccagcgc ccgtttcgtg ctcatcccac ggagttgctc ctattggtct gggtgttgag
    11701 cctctttacg gccttcagtc tggcgaccct caaacgggag ccctacctca cgacgatcgt
    11761 gcccggcatc ggcttgatga tcgggtacta ctatcaccgg ctgttctcgt cggccgagga
    11821 ctctgctgcg atgacgcccc tgctccgtgt gttgctgggt gtgttggccg tgacgttcgc
    11881 cgccgggatg ttcttcggcg cagcaccgct tcgccgccgg tggctggtgt ctactgccgt
    11941 ggtgtcgccg atgttcatcg tgacgatcgt ttcactggcc ggcttgctcc tctatgccgt
    12001 gcttcgatca cgcgtacgcc tcgcactcgg catggtcggg gctcttgcgg tcgcttacgt
    12061 ggtgctggtg gtaaactacg tgtttcccgc gatcgatcag gccgcgtcgc cccggaaggc
    12121 cgtcgaggaa atcaaaaccc tggccctggg aactcagccc gccctgttca tgtatatccc
    12181 ggggtggccc aagaatgaag atgccctgta ttatctgaag cgcgacaatg tggtgccgga
    12241 tctgccgagt caggatgcgg tgcgtgaagc ggtgcgcaca catggggcca tccgggtggt
    12301 gactgaagaa caacatgccg ctgcgctgca gtcgcaagca gggttggtcg tagggaagct
    12361 gcaggagttt cggcaacccg gtcgcaaaca tctcttcctg ctttctgtac aggaacagac
    12421 ctcagctcgg gtgctttgag aggagcgagc gatgggccgt tcgatgccaa ggtagccgct
    12481 gtgtcttgtt ttgatccatt ggccctgttg cattctgcca cagagccaag gattcgctct
    12541 gtagcagtcc tccccgattc cgatcccctt ctcctcctct cgcatctcca tctctgatct
    12601 tgctgtaagc tttcagctgc ctctgcgccg ggaagaagga gggctccatc cttgcacttc
    12661 cgatctggaa ggcgtcgatc ttgagagggc atctgtgcct tggcgattcc ggatgagacc
    12721 tggtcgattc accctattga gcgaaggagc gagcgtgcat ccgatccatc ccatggtcgt
    12781 ccattttcct attgcattgt tgttggcgag caccctgttc gatgcgttgg cgtttcggtg
    12841 gcggaggcac cagtttcggg acaccagctt gtctctgctg gtgctcggca tcctggcggc
    12901 cggagcggcg gtgctcaccg gacatttcgc cgaggaggcg gtggaacgca gcggcatccc
    12961 gaaacaggcc atcgaaattc atgaggaact gggcggctcc gtgttctggg tatttctcgg
    13021 cctgctcggg ctgcgtctgg cctcttgttg gggttggatg cgtgaacagc cgatgctggt
    13081 gctggtcatc ggcctgagcg gcgggctgtt gttgttgatt gcgagttatt tcggcgggga
    13141 tctggtctat cgattcggtg ccggcgttct gccacgatga gcgcggttcc aggtcgggtg
    13201 tggcctgcag cgctttcgga gggaactcac atgacgacac gtatcgtgat gttgttgatc
    13261 ctgtgcatgc tgcctgccac ggcccaggcc gatgcatcgt tgtctcgcgc gccgtggaag
    13321 gagcgggccg tcttgcgtac ggggcaagtc gtacagggag actattttgc attcggcccg
    13381 catgtggaaa tttccggcat cgtcaacggc gatgtgatcg tggcgggagc caaagtgatc
    13441 ctgtccggca ccgtggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtca
    13561 acggcgatgt gatcgtggcg ggagccaaag tgatcctgtc cggcaccgtg gctcaggatg
    13621 ccagagtcgc cggcgctcag gtcgtcagca tctccctaac tcaccgacat gttgtatctc
    13681 gggaacgttg ttcgtcagcg agcgacatgc cagtgatcca aaaccaccaa caagactctt
    13741 aaaagtattt agccacatcg ttgaggaagt ctgcgacaac caggcgggaa tgaatgtacc
    13801 gaaactcccc atgatgcagc gcgatccgtc cgcaccgtcc tctcttcctc ccgcgtaagc
    13861 tcccgttgtt ctgaggatct gaagaaaccc gccccgagaa tttgtaactg gcacccatgc
    13921 tgcagtgtgc gggatccata ggaacgtcat gtgattgcgc aggaggggaa atgatgagtc
    13981 accatgagac ggatgatcga gtgctggaag tgattgtgcg gtcacagggg cgcactctcg
    14041 atgatatcgt gctcgaatgc ccggagctca catggaatca agtcttcatc gtcattgatc
    14101 ggctcagtcg agagggggcg atccggctga tgcctaaaga gcgagggtgc tacacggtcc
    14161 atctcgcgag caatgtcttc cacgacgcgc ggcagtacgc cagagtgtga ggtgagagga
    14221 cgggggcctg aacgtggatg tcttctggcc cagtgttcgc ggtggtgttt tcagtgctga
    14281 tgcgcggaac gtttctgcgg gcctattggg tgactccatc acatgcgcgt gcggagttgc
    14341 acagaatgac gggctgttgg cgttctttcc agcaaggaga ctcgtacgga gggaaaaact
    14401 ttttagcgtt aaaggagacg ctcatgaagg ctcagacgac acaaccgttc cgggagaaga
    14461 aaccgcacac gatgcaggtt gagcagccta acgtgcctgt gtcaactcgc aaagcagcag
    14521 gttcggcaca agctgcagtc gacgatttac acgcccgcat cacggcacga gcctatgaac
    14581 tgtatgttgt gcgcggctgc cgaaatggct accacctcga agattggctg gatgcagaac
    14641 gagtcattct ggatcgcgca gtcaccaaat aggatttgcc gtggcgttga cgcgccgagc
    14701 aattcattgt ctgtcgagac aagccagggt ctccacaagg cagtgcagcc aagaacatca
    14761 ccctgagcat tatcaagacc aatatcggtt ccatcagcgg tcacgtgtgt cccttgcggc
    14821 aattgctgga ccttgcgcct tagtcacgag gtcatccagt cagatcaaga aaggtgtatt
    14881 tctggggagt ttcttcacag cgtcatctcg aaattgtttc caatctcccc aatgtccaac
    14941 attgagtcgc ggagttgatg tgacagtaac ttcgtgaaat tgcagtgctc aacggctggc
    15001 gcaggagacg gagtggatct tgtcggagat tcgacgtctc ccaacaccgt acccaagaga
    15061 ggtgtccatg gagagccaga cggataggaa ccgtcggatc gcgggcgtcg tcattcgttt
    15121 tcggagctgc gagcgtgatg agatcgttct tgaatgccga ggcctcgtgt ggaattaggt
    15181 cttcctggcc gttgatcctc tcagttgaaa cggcggtgta gtaccgcagt tgagccgacc
    15241 aggtatctac acactgcgcg taccagagca cgttggagcc gagcgtatga tcagcatggt
    15301 cggtggatgg tgatgagaaa cacgatgtgg caaatccgca ttcatgggcg gggtgggcaa
    15361 ggggtcgtga cggcggcaga actgctcgcg atggcggcgt tcatggacgg caaatatgcc
    15421 caggcctttc cgagcttcgg gtcggaacgg atgggagcac cggttgtggc gttctgccgc
    15481 gtcgctgagg gagccatccg cacgcgcgaa acggtgacgc gccctgatgt tgtcattatt
    15541 caggatccga cgctcctgca tcaggtcgac ttgttcggag ggctcgcccc cctcgggttt
    15601 gtgttgatca attcgaccca gaccctcgat gcactccaca ttgaggaatt tctctcaacg
    15661 ctgcccctcg gccatgtgtg tgcgttctct gccacggacc tcgggctgaa atacctcaag
    15721 cgacccattg ccaatgggcc actgctgggt gcgttcgccg cgatcaccgg actgatccgg
    15781 cttgcctccg tggagtcagc gattcagcag aaattcccag gtgcggtcgg ggaggcgaat
    15841 cgggctgccg cgcgggaagc gtttgacgtt gtcaccactt ctattactga acaaagggga
    15901 atcgtacacc atgcgcatgc agattgaggg ttcccaagcc gtggctgagg ctattgcact
    15961 ctgccaacct gaggtcattg cggcctatcc catttcccca cagacgcaca ttgtcgaacg
    16021 gttatcacgc aagaccaagc agggccacat cggacgctgc cagtttctga acgtagaatc
    16081 cgagtttgcc gccttgagtg tcctgattgg cgcctcggct tccggcgcga ggacctacac
    16141 ggcgacgagc agtcaggggc tgctctttat ggccgaggcg gtctacaacg tagccggact
    16201 aggactcccg attgtcatga ccatcgccaa ccgggcgatc ggcgcgccga tcaatatttg
    16261 gaacgatcat tcggacagca tggcgttgcg cgatgctggg tggattcagt tgtttgccga
    16321 ggataatcag gacgccgtcg atctccacat tcaagcgttc cgtctggccg aatcgctgag
    16381 ttgcccggtg atggtctgca tggacgggta tgtgctgacg cacgcctacg aaccagtgga
    16441 cgtgccggac gccgttcagg tgaatgccta tcttcctccc tatgatccgg tacaggtcct
    16501 tgatcctgaa cgtccggttt ccatcggcgc catggtgggg ccggaggcgt ttaccgaagt
    16561 taaatatttc tcgcaccaac aacatcttcg agcgctagag gtcatgccgg aattggtgga
    16621 ggtcttcgag caacattttc agcgacgatc cggcggactc tgctccgctt atcgcatgga
    16681 ggatgccgac atcgcggtga ttgccatggg gtcggtcatc ggaacgatca aagacgccgt
    16741 ggacgaactg cgaaacacag gtgtccgggt cggctgcgtc aaaatcggat gctatcggcc
    16801 ctttccgaag tttcaggtaa gcgcactgct agaaaaaacg acgcgggtga ttgtagtgga
    16861 gaaagacctt gccgtcggca tgggagggat cgtctctgcc gacgtccaat cagcgctcca
    16921 aggacggcag atttccgtcc acaccgtcat tgcaggattg ggaggacgtt ccatctccac
    16981 atcttcgttg aagcacatga tcctggaagc tcgtgcagaa cgtctgacag aaccgtactt
    17041 cttggacctc aacaagacag tgatcgagca ggagctcgct cgacggcgcg acatccgacg
    17101 ggctggtcca gtcgcggagc atatgctgca aaccatgaat gtgatcggaa cgaaagtgag
    17161 ttgatggacg aggcgatgac gacggaacgc ataaagtttt atcagaccgg caccttcacg
    17221 gctgggaacc gtttgctcga tgaagcgacg cggaccgtcc aatcccggtt ggatcggacc
    17281 aattcgctca actccggtca ccgagcctgc caaggttgcg gcgaagcgct gggggcgcgt
    17341 tacgcgctcg atgccgccat gcgtgccagc cacaatcaat tgattgccgt gaatgcaacc
    17401 ggctgtctgg aggtcttttc cagcccctat cccgaaagcg cttggcaaat tccctggatg
    17461 cattcgctgt tcggcaatgc accggcggtg gcgagcggga tcgccgcggc gcttcgtgcc
    17521 aagaggcgtg acgacgtgac ggtgatcgcg caagggggtg acggagcgac ggtcgacatt
    17581 gggttcggtt gtctgtccgg gatgtttgag cgaaacgatg acgtcctgta tctctgttac
    17641 gacaacgaag cctacatgaa caccggcatc caacgctcgg gggcgacccc tccgttcgcc
    17701 cgaaccaaca cgacgcaagc cgtgggtgag catcccggca acccgattgg gaacgggaag
    17761 aacgtgccac ggattgccct cgcgcatggg atcccctatg tcgcgacggc ctctgtggcc
    17821 gatctccatg acctggaacg aaaagtcacg aaggcgatcg gtgttcgcgg ggctcggtat
    17881 atccatgtgc acgtgccttg tccgctgggg tggggctcgg accccgcaca cacgatgaag
    17941 gtggcgcgcc tggcggtgga atccgggctg ttccctctgt tcgaggccga attcggaaaa
    18001 gtcatctgct ccacgccgat tcgccgcaaa gttcctgtcg aacgctatct ggagctacag
    18061 actcgattca agcatctgtt cggtccgccg caggacacgg aagccctcgc cgcgatccaa
    18121 gcaatggcag atgacaacat cgctgtttat ggactgatgg aatagaggca cctatggaac
    18181 gcacaccatt tgccatcacg ctcgatccga acacgagcct ggccaaccac acgggaaact
    18241 ggcgcacgat gaaaccgatc tatgtcgatc gcttgccacc gtgcaaccac gcctgcccgg
    18301 ccggcgagaa cattcaggcc tggctctatc atgctgaaga aggggcctac gaagaagcat
    18361 ggcaacagct catgcaggac aacccgatgc ccgcgatcca cggccgcgtc tgttatcacc
    18421 cctgtgaaag cgcctgtaat cgtgggcata tcgatgaggc cgtcagcatt cacgcggtgg
    18481 aacggttcct gggtgatcag gcgatcgcgc aagggtggcg ggtggcgtgc gctccacgga
    18541 cggggaagaa cgtgttggtc atcggcgccg gtccaagcgg actctccaca gcctatcact
    18601 tggctcggat ggggcatcag gtcaccattc atgaagccgg tccacaagct ggcggcatga
    18661 tgcggtacgg catccccaag taccgactgc cgcgtgagat cgtggatgcc gaagtcgcaa
    18721 gaattcaatc catgggtgtc acgattaggc tgaacagccg agtcgacgat cttgaggcga
    18781 ccatggcgga aggacgattc gacgccgcct ttctcgcgct tggcgcgcat ctagcccggc
    18841 gcgtcgaaat tccgggggtc gatgccaagc gggttctcga tgccgtcacg ttcttgcgcg
    18901 acatggaagg ggcggctcgc cccaagctag gacgccgcgt cgcgatctac ggtggaggca
    18961 acacggccat cgacgtggca cgcagtgtcg agcggttggg gatggaaccg gtcatcatta
    19021 tttaccgtcg agatttgggc catgcgccgg cgtttgccag cgaaatcacg gaagcccagg
    19081 acgaaggcgt gcagttccaa tggctacgaa ggatcgcgca aggtgacgaa tcgacattga
    19141 cagtcgagga aatggcactc gatgaacaag ggaaaccaca accgaccggt cgcttcgaaa
    19201 ggatcgaagc cgatagcgtg attctggcgt tggggcaacg ggccgatacc ggttttcttc
    19261 gtcgggtatc cgggttgcgt gtacgggagg acggaaccat cgaagtggat gggacgatga
    19321 tgactggccg tcccggtatc ttcgccggcg gagacatggt gccaggcgag caatccgtca
    19381 cggtggccac cggccagggg aagaaggccg cccgccacat cgatgcctat ttgcgcggct
    19441 ccgcgtacgt caaaccggaa gctcatgagc cggcgacctt cagccgtttg aacacctggt
    19501 actacaccga cgcagacgcc tcgaagcagc cgttactgaa tctggtgcgg cgtcggtcga
    19561 gttttgatga gatcgtcggc ggccttgatg cggacaccgc gctcttggag gcacgccgct
    19621 gtctgtcctg cggcaactgc ttcaactgtg acaactgcta tgcggtgtgt cccgacagcg
    19681 ccgtgatcaa gctcggacct gggaagcggt atgcgatcaa ctacgactac tgtaaaggat
    19741 gcgggctctg cgcggaggaa tgtccctgcg gcgccatcga catggtgaga gaagtgaaat
    19801 agaggggacc gacccgtgta tgtgatgctg gggtggcagc tcttctgccg acagaactcg
    19861 cacaatcgat gagagaggat ggcagccatg gccttagcac aacgagcatg cagcggcacc
    19921 gcattggttc tgtgcgctgt tctggtcggc ccggtacatg cggcgaaggt cagtgatgcc
    19981 gccgcgacac atgagtggat gtcaacaccg gtgcgaaccg ccgcagaaca gatgcttgcc
    20041 gcgatgcccg acgactacta cagcatcaag ggcgtcgcgg ccgagaaggc gatcgccacc
    20101 ggcgtacccc acgtgctcga tgtacgcgaa ccaaacgagt ttgcggttga gcatattccg
    20161 ggcgccaaaa acgtcccgct ccgcagtctc atgacagcca tcgacgacct cccgggcgac
    20221 aaagcggcgt cattgattgt ctactgcaaa accggacatc ggggtgctat cgcgttagcc
    20281 gtactgagga tgctcggtta cgcgaacgtc ctgagcctct acggtggatt ggacggctgg
    20341 aaggctgcgg gcttcgccat tgaaaagtag cgccacgaga agagtggcag aaggagttga
    20401 gacatgtcgt ataccgccac cacgacccaa gccgcgatcc gctggttcga ggacattgga
    20461 atcgacgata tcccgttggt tgggggcaag aatgcctcgc tgggcgagat gtaccgggaa
    20521 ttggcctcac ggggcgtgaa ggttcccaat gggtttgcca ttacggccga ggcctatcgt
    20581 tcgtttcttc gcacgaccgg gttggatcag cggatcacac agataatccg tgggctggac
    20641 acgcgcgacc tggacaatct ccgtcagcgg gggagacaga tccgtcaggc catgctggcg
    20701 gccagtctcc agcaggatct cgaacgagcc atcgtagagg cctatgaccg gctgagtgat
    20761 gggcatgttg atctggtgga tgttgcggtc cggagcagcg caaccgcaga agaccttccg
    20821 gatgccagct ttgccggcca gcaggaaacg tacctcaacg tgcagggcca tcaggcgctc
    20881 ctggatgcct gcaagcggtg ctttgcttca ctgtttaccg accgcgcgat ctcctatcgg
    20941 gtcgataagg gattcgatca cttcaagatt gcgttgtcga tcggtgtcca gcgcatggtg
    21001 cggtcggatc tgagcgcatc agggatcatg ttttcgatcg atacggagac cggctttcgc
    21061 gatgccgtgc tgatcaatgc ctcctatgga ttaggagaaa acgtcgtgca aggggccgtg
    21121 aatcccgatg aatactatgt cttcaagccc accctcaggc agggattccg gccgatcctc
    21181 cagaagatgc tgggcagcaa agagttcaag ctcgtgtacg acgtcggcgg gagcaagttg
    21241 gtcaagaatg tgccggttcc accagatgat cgcgccagat ttgcgatcac agacgacgag
    21301 attctcacgt tgtcacgatg ggcctgcatg atcgaagacc attacagtgc caagaagggt
    21361 cggaccagcc ccatggatat ggaatgggcg aaagacgggc atacgggcga actctttatc
    21421 gtccaggccc ggccggaaac cgtgcagtcg caaaagcgcc aagactcgct tgaactgtat
    21481 cggttaggtc ggaaaggcaa ggtgctgctc accggccgaa gtgtcggcag caaaatcagc
    21541 cttgggccgg ttcgcgtcgt caaacacctc caacagctgc gagatttcac gagcggcgat
    21601 gtcttggtca cggacaagac ggatccggat tgggagccgg tgatgaagaa ggcgtcaggc
    21661 atcatcacga atcgcggcgg cagaacatgc catgccgcca ttgtgagtcg agaactcggc
    21721 gttccggcta tcgtcggaac gggcaatgcc acggaggtgc tgcgggactg ccagatggtc
    21781 acgctgtcct gtgccgaggg tgatacggga tttgtctatg aagaggcact ccccttcacc
    21841 gtgcagcgcg ttgagctcag cgacctcaca agaccgagga ccaagatcat gatgaacgtc
    21901 ggaaacccgg aagaggcctt cgcgttgtcc ttcatcccca atgacggcgt cgggctggcg
    21961 agagaggagt tcatcatctc cacctatata aaggtacatc ccctcgcact cctcaactat
    22021 gaccggttgg acgatgctgc ggtcaaggct gagatcgacc gactgacagt gggctacgcc
    22081 gacaaggccc agttcttcgt ggacaagctg gcccaagggg ttgcgatgat tgcggcagcc
    22141 ttctatccaa aggacgtcat cgtccgactc agtgacttca aatccaacga gtatgccagc
    22201 ctggtcgggg gaagaggcta tgagccgact gaggagaatc ccatgattgg cttccgcggc
    22261 gcctctcgct actacgattc acgctatcag gccgggtttg ccttggagtg ccgtgcgatg
    22321 aagaaagtcc gtggcgagat gggactcacc aacgtgaagc ttatgatccc gttctgccgc
    22381 accgtcgaag aagggcgact ggtccaggct gaaatggcga agcatgggct tacgcgcggt
    22441 gagaacgggc tcgagatcta cgtcatgtgc gaagttccga gcaatgtcgt gttggcggat
    22501 cagttcgctg aggtcttcga cggattctcc atcgggtcca acgatcttac ccaattgacg
    22561 cttggggtgg atcgagattc cgagatcatc gcccatctgt tcgatgagcg taaccaggcg
    22621 gtgacatcaa tgatggcatc ggtgattaca acggccagga agaaggggaa gaagatcggc
    22681 atctgcggac aggccccgag cgactatcca gaggtggcct cgttcctggt cgaacaaggc
    22741 atcgacagca tctcgctcaa ccctgacgcg gtcctcaaga ccacgatggc cattctgaaa
    22801 ctggaacagg gtttacagag gggacccgca caacgaactg gctgagacac acagggagga
    22861 cggaccgatg aaacgaatca tctctgatct accccatgat cgctatcgag aacgaacggt
    22921 cttggttcgt gtggacttca acgtgcctct cgatcacggt cggattcggg aggactaccg
    22981 ccttcggcaa gccatcccga caatcgaata cctctcccag cgtggcgcga aagtgatcct
    23041 cgcctcccat ctgggaaagc ccaaggggaa agtggtgcca gcactgtcgc tgaaaccggt
    23101 tgctgatcgc cttggacaga ttctgagagt tccaacagta aagttcgtcg aggcggcggt
    23161 cggcgagtcg gtgaaacaaa cgctcaccac gttgaagcct ggagaggttc tgctgctcga
    23221 gaatctgcgg ttcgaaccag gcgaggaggc gaatgatccg gtctttgcca agcaactcgc
    23281 cgcactggcg gagctctatg tgaacgatgc ctttggcacg atgcaccggg cccatgcttc
    23341 gacgtacgga gcagcacaac tcttcacaga ccgcctggcc ggttttctcg tgcgtaagga
    23401 aatggatgtc ctgagtcagg tgcgcgatcg ccccgttcgt cccttcaggg tggtggtcgg
    23461 tgggatcaag atcaaggaca agctgtcggc gttgaaggcg ctgctgccga aggctgatac
    23521 catccttctg ggcggcggaa tcgcctatac gttcctggct gcccaaggcg tggcgacggg
    23581 tgattcgccg gtcgagcctg aattcattcc ctgggccaaa gacgtgctgg cacagtatcg
    23641 aaagcacatt ctgctgccgg aggaccacgt catcgccgcc aacgcggagc agcgggacag
    23701 actgcaactc gtccatcgag agatccctgc aggctttatg ggattggata tcggccgcga
    23761 tacgtcgctg accttcgcgc acgagttgct gcaaggaacc gggaccatct tctggaacgg
    23821 tcccctcggc gcgttcgaag tcgatgaatg tgccgacggg acagtcgaca tcgcgcgggc
    23881 catggcactg gcccattggc gtggcgcctt cacggttgtg ggaggcggcg acaccgtggc
    23941 ggcggtgcga aaagcggaag tactggagac ggagattagc catgtgtcaa caggcggagg
    24001 ggcatcgttg aaattcatcg gcggtgaaga actacctggg atcgccgtgc tcagcgagca
    24061 atcccagtaa tggagaagga gggaacccat gagaaaggtc aaagtcgcag tcaatggcta
    24121 tggggtgatc ggcaagcgcg tggccgatgc ggtgaagcaa caggaggata tggaattagt
    24181 cggcgtggcc gatgtcgtga ctgactaccg gatcaagacg gcagcggtcc ttgggctgga
    24241 tatctatgct gcgctgccgg acaaggccca ggcgatgcgc gcagccgaca tcccagtcgc
    24301 tggcaccttg gatgacttgc tccggaaggt cgacgtggtg gcggattgca cacccaagaa
    24361 aatcggagct gccaacaaga tactctatga gcaggcaggt gtgagggcaa tctaccaggg
    24421 gggcgaatca catgcgctga ctgggcattc cttcgtggcc caggccaatt atgaaacggc
    24481 catcggtcgt tccagcacca gggtcgtttc atgcaacacg acctcgattg tgcgaacgct
    24541 cggtgccctg aagaaggccg gactcctcaa acgcgcgcga ggcacgctga tccgtcgagc
    24601 atcggatcca tgggaagcgc atctggacgg catcatgaac accgtcattc cggaaaagac
    24661 cataccgagc caccagggtc cggatgcgaa gaccgtcgat ccggatttgg atgtcgtgac
    24721 gatcgcggcc aaagcgtccc ataacagcag ccatcttcac acctggtggg tggagctgac
    24781 ccgttcggcg agcaaagagg aattgttggc cgcattccgc ctggcaccac ggatcgcctt
    24841 cattcgcatg cacgaggggg tggcggcgct gaatagcacg atcgagctga tgagcgactt
    24901 gggacgtcga cgcggagatc tgtgggaagt ggcgctgtgg gaagatgtgc tcacggtgaa
    24961 cgggaacgag gcctactaca cctaccaagt gtttaaccaa gccatcgtcg ttccggagac
    25021 catcgatgcc attcgggccc tgacgggact ggagtcacag gcagagcggt cgattcagaa
    25081 aaccgatcag gccttgggga tcgccaaaga atttctgaga agtagcgaac acgtgctgat
    25141 ggcataagca tgtagcctca ggaggcatgc aggcatgaga ccggggcttt cggaagccga
    25201 agctgacagg tcgatgcgtc tgagaagtca gatcgcatgg agaaagcccc aactcaatcc
    25261 agatataagg aggagcaaac catggcccag accatgaaag catttgtcat gaagaagctc
    25321 ggctcggtgg ggatgatgga gaagccgatt cctgatctcg gaccgaatga tgcgatcgtc
    25381 aaaaccacgg ccgcgctcat ctgtacgtcc gatgtccata cggtcggcgg ggcgatcggt
    25441 gaacgcacca acctgaccct gggccatgag gccgtggggg tcatttacaa attgggcagc
    25501 gccgtgaagg gatttcgtga gggcgatcgg gtcgccgtca acgcgatcac gccctgttac
    25561 ctctgtgaga actgtttgcg cggttacacg tcgcaatgta ccgacatgct cggtgggtgg
    25621 aagttcgcga acgtgaaaga cggcagtctc gccgagtatt ttcatgtgaa cagtgcacag
    25681 gcgaacttgg cgccgatttc taagggtctc accgacgagc aggccgccta ctgcgcggac
    25741 atgatgtcca ccgggttcat gggggcggag catgcgaaca ttcccatcgg cggctctgtg
    25801 gcggttttcg cccagggtcc ggtcggcctc atggccacgg tgggatcccg gttactgggg
    25861 gccggcttgg tcatcgcagt ggaggccgtc ccggagcgaa agaaactggc gaaagagttc
    25921 ggggcggatg tggtgatcga cttcacggaa caggatcccg tcgaagccat tctcggcttg
    25981 acgggcggac aaggcgtgga ttcgtccatc gaagccctcg gggctcaggc gacgttcgaa
    26041 gcctgtatca aagcgacgcg tccgggcggg accatttcca acgtgggcta tcacggagag
    26101 ggacaatacg tacagatgcc gcgcaaagaa tggggcgttg ggatgagcga taaggttatc
    26161 aggacgggac tctgccccgg tggagcggaa cggatgaaac ggctgatgcg gcttttagaa
    26221 accgggcgtg tcaatccgct cccactgacg acgcatcgat tcagattcag cgatgtggag
    26281 aaggcctttg agctgatgcg aacgaaggca gacggcatgt tgaagccgct catcacgttt
    26341 gcgtgaaggg aagctcccaa ctcttttggc gtgccctgca agaaaattgg cgcgccgtgc
    26401 tagtgatcga tccgggtaaa gatcctactt cgtcggataa atgaataatc cattcgactt
    26461 caaggccgtc gactaatgta aaaggtccgc gacgagcaat atcaggccgg attaaaggtc
    26521 gaataaatgg aaacagccat cgggtctggt ttatcagacc gtataccgac atcttttgcc
    26581 tagaggagga tatatccatg tacaagacga aggcttactc cgctgccagt gcgacatcgt
    26641 cgcttgtctc tacgacaatc gctcgtcgag atccaaccga acatgacgta cagattgaga
    26701 tactgtactg cggcatctgt cactccgacc tccattcggt acgtaacgat tggagcagcg
    26761 tcatgtccac tacctacccg attgtcccag gtcatgagat cgtcggccgt gtcaccaagg
    26821 tcggctcagc ggtgacgaag tacaagcccg acgaccttgc cgcggtcggc tgcctggttg
    26881 actcggacgg gacttgcccg aactgtacag ccgggcttga gaacttctgc ccgaaccaga
    26941 ccttcacgtt cggctcaccg gacaagcaca agaccgcccc ggtcacctac ggcggctact
    27001 ccgacagcat cgtcgtcgac gagcgcttcg tcctccgcgc ccccaccaac ctcaacggcg
    27061 ccggcaccgc acctctgctc tgctctgcgc tgggatcacc acctactcac ccctgcgcca
    27121 ccacggcgtc accaagggta agaaagtggg catcgtcggc cccggtggct taggccatat
    27181 gggggtgaag ttcgctcggg tgttcggggc ccacgtggtg gttttcacca cctcgcccag
    27241 taaggcagaa gatgcgctgc gcctgggcgc gcacgaggtc gtcgtatccc gtgatgcgaa
    27301 cgagatgaaa aagcacaccg gcagctttga cttcattctc gacacggttc ccgtcgatca
    27361 cgacatcaac caatatcttc atctcctccg cctcgacggg aacctgaccc tcgtcggagt
    27421 gccgtctcaa cccctcgcgc tgtcgtcgtt cgcgttgatc tttgggcgcc gcagtgtgtc
    27481 cgggtcgaac atcggtggta tccccgagac ccaggagatg ctcgacttct gcggcgaaca
    27541 taacattacc gccgatgtcg aagtcatccc gatccagaat gtcaacgaag cctatgacag
    27601 actgctgaag tccgatgtga agtaccggtt ctccatcgac atggcgtctc tcaagactga
    27661 gtgaccgacc cgatactcga aaggaaaaac aaatgcaaaa aggacaataa ggtgtcagca
    27721 acctttgagg aggctatatc catgcaaacg accactttga gcaacggcct gcacatgccg
    27781 atgctagggc taggggtgtt tcaggccccg cccgatgtca cggctgccgc cgtccaagaa
    27841 gccttgcggg tgggctatcg cctcatcgac acggccgccg cctacgccaa cgagcgcgaa
    27901 gtgggcgaag gcatccgccg ttcgggactc gcccgcgacg acatcttcat tcagacgaag
    27961 gtgtggatca gcgactacgg ctacgacgcc accgtgcatg cgttcgacaa gagcgccggc
    28021 aagttggggg tcgatcaact cgacctgctg ctcttgcatc aaccgctcac cagccgtttc
    28081 gatctcaccc tcggcgctta caaggcactg gagaaactgc tggccgacgg caaggtgcgt
    28141 gcgatcggcg tgagcaactt catgccggac gtgctgcaac gccttctggc agaaacatcg
    28201 gtggtgcctg cggtgaacca gatcgaggtt catccctact tccagcaaaa ggaactgcaa
    28261 cgtctccacg ccgagcacgg catcctgacc caggcctggt cgcccctcgg cggcatcacg
    28321 tcctacgggg gcggcgagaa gaggacgttc gacgacccca ccctgcagca gattgcgcgt
    28381 gagcatggca agtcagccgc gcaagtgatg ctgcgttggc acctgcagga gggccgttca
    28441 gtgatcccca aatcgaccaa gcccgcgcgc atcgccgaga accttgacgt gttcgacttc
    28501 gaactcacca aggagcaggt cgcttcaatc gacgcgctcg atacaggcgt gcgcagcggc
    28561 ccggagccgg acagcatcac cttggagaac tacggtcgcg agattccaga ggcctgtgga
    28621 aaataaagtg tcaggaacct tttattggct caacatgcgg caagaccaga aatggttcct
    28681 gacacctttc caccccgccg ctgctgatcc cccgcatcgg agaagttgcc gagatcggga
    28741 gcgccgctca gacattccct cgctggtggg aagacgtctc accttgatct gccttgtgac
    28801 gacttgaaag gagctatcaa ccatgagcaa gaccattttc attaccggcg catcttcggg
    28861 gctgggcaaa gccacggcca aactgttcgc ggtcaagggc tggaatgtgt tggcaacaat
    28921 gcgtcagccc gccaaggaaa ccgagctgac ccagttcgcc aacgtcaccg tgctgccgct
    28981 ggacgtgacg cgctcggaac aggtgagcga aacgatccaa agcgcgctga agtccagcga
    29041 cgtggacgtg gtgttcaaca acgcgggcta cggtctggca ggcccgatgg acggtattga
    29101 cgatgcccgc atcgtgcggc agttcgagac caacgtcttc ggtgcgatgc gcgtcacgcg
    29161 cgagttcatc ccgtacttca agcaaaagcg cagcgggcgc ttcatcacca ccacatcgct
    29221 ggcgggcttg atcgggctgc cgctcaactc ggtctacaac gccagcaaat gggcgctgga
    29281 aggctggagc gaagggctgt cttacgagct ggccccgttc ggcatcaagg tcaagaccgt
    29341 cgcgcccggc gccgtcatga ccgacttcat gagccgctcg ctcgaccacg tgcccgcgcc
    29401 aggctatgac gccatgaacg aggccacggt gaagttcttc gtgcccgaca tggcgaagat
    29461 ttccagcgcc gaatccatcg cgcaagtagt ctgggaagcg gccaccgatg accaggatca
    29521 cctgcgctac gtcgccggcg atgacgccat cgacatctat gcgctgcgcc agaagctcgg
    29581 caacgaaagc tttcgccaac acctcgccag cctggtgctt tgaggctgcc cattcaccgt
    29641 caagaaagcg agatacacaa gatgaagccc caagtcattt gccacatgct gagttccgtg
    29701 gatggccgct tgcacgtggg ccgctacacg ccgctgacca gcggaaagac ctccaccgag
    29761 gcatcggccc cgtactacga atacagcgcg aagtttgatg cggatgcgca gatcatcggt
    29821 ggacggacgg cccgcgaatc ggcgctgccc ctgcggtacg agggcgatgt cgctacgcga
    29881 cccgcgcatt tccagacctt cgtgggtcat cggcaatcga agcgatccct gatcgtgctc
    29941 gaccctcagg gaacgatccg ctacgacagt gacaacgtgt ccggtgaaaa cctgatcgct
    30001 gtcctgggcg aaagcgtctc gcagcagtat ctggaccatc tgcaagcgca aggcgtgtcc
    30061 tatttgtttg ccggccccaa gggcgacgac gttcccaggc tcctgggcac gctatcggcg
    30121 gacttcggca tgacccgggt gctggtcgaa ggcggcggaa ccatcaacgg cgccttcctc
    30181 aaggccgggg tgatcgacga actgagcctg atgctttatc ccggcatcga cgggttggcg
    30241 gggattccgt cgatctttga atgccaaggg gcgccgggcg agaagccggc cgaggggcag
    30301 tcgatggagc tggtggccgt cgagactttg ccagacggca ttgtctggct gcgctatcgc
    30361 tttcatcggc aatgacgccc cccacacatt gaggttgcgc tatgcctatc acctttctat
    30421 tggttcggcg acctccatag aggaaagaga aaaccatgaa gtacatcaaa cttggaaaca
    30481 caggcattga cgtctcgaag atttgcctcg ggatgatgag tttcggcaaa ccgggcagtg
    30541 aacacggcct gttcccctgg gccgtggact tcgaggatgc caagcccatc ttcaagaagg
    30601 cgatcgaact gggcatcaac tacttcgaca cggccaacgt gtaccagcta ggcaccagcg
    30661 aagaggtgac cggcaaactc atcaaggaat tcaagctcga ccgggacgac ctcgtcgtgg
    30721 cctcggctgc atgggcctga gcttcggcta cggtcctgcg accgacaagg gggatgccgt
    30781 caagctcatc cgcgccgcgt tcgaacgcgg cgtcacgttc ttcgactcgg ccgaggccta
    30841 cggcgcggtc aacgaagaga tggtgggcga ggccgtcgcg ccctttcgcg acaagatcat
    30901 catcgccacc aagttcggct tcaaggatgg caattcgcag gccgggctgg acagcacgcc
    30961 tgcacgcatc cgcctcgtgg ccgagcagtc cctgaagcgg ctcaagacgg accgcatcga
    31021 cctgttctac cagcaccggg tcgatccgaa cgtcccgatg gaagacgtcg ccggtgtggt
    31081 caaagacctg atcacagaag gcaaagtcaa gcacttcggc atgtcggaag ccggcgtcgc
    31141 gatgatccgc cgcgcccatg ccgtgcagcc gctgaccgcg ctgcagagcg aatactcgat
    31201 gttcacccgc gaacccgaga cggagatcat ccccacgttg gaagaactgg gcatcggctt
    31261 cgtgccgttc agcccgctgg ggaaagggtt cctgacaggc aagatggacg tggacacgaa
    31321 gctggacgcc aacgacttcc gcaacacgct gccccggttc gaagccgaga atcgtcgcgc
    31381 aaatctggcg ttcgtggaat tgctcacaac catcgcgaat cggaagcagg tcacccccgc
    31441 gcaaatcgcg ctcgcgtggc tgctggcgca aaagtcgtgg attgtgccga tccccggcac
    31501 gaccaaactg caccgactgg aagaaaacct cggcgccgcg tcggtcgatt tgaccaccga
    31561 cgacctgcgc gagatcgagc aaggcgtctc gaagctgacc gtccagggcg cgcgttatcc
    31621 cgaaaaggtc gaacagatga ccaaccggtg aacgctcgcg ccacaacagc atgagccgtg
    31681 caccagctca tgaacgcttg gaccgccggc tcaccccggc acgaagccgt gatggagtga
    31741 ccacattaat cttaaagaaa ggatcacatc atgagctacc aaagtgtaaa tcccttcgac
    31801 ggaacaaccg tcaagacgtt tgagcaactg acagatgcgc agctcgaaac ggcgcttgaa
    31861 accgccgcaa actgtttcga gaactggcgg cgcaccacct tcgccgagcg tggagccatc
    31921 gcggcaaaag ccgccgccat catgcgggcg cgcatcgacg aattcgcccg ccctgtgacg
    31981 ctcgagatgg gaaagctcat cgccgaatcc cgtgccgaag tgcagttaag cgcggacatc
    32041 atcgactact acgccaagac cgccgagcgt tttctcgccc ccgagcctct tcagcccggt
    32101 tcgggcgaag ccaaaatcga aagcgctccg ttcggcgtgc tctttggtgt ccagccctgg
    32161 aattttccct actatcaact cgcgcgcttt gccgcgccga atctgatggc cggcaacgtc
    32221 gtgatggtga agcactcggg caccgtgccg cagtgcgcca tcgcctttga aaagttatgg
    32281 cttgaagcag acgcgcccgc gggcgcttac accaacctgt ttatttccta tgaacaggtg
    32341 aatcgggtga tcgaggatcg ccgcgtcaag ggagtggcgt tgaccggcag tgtggacgcc
    32401 ggcaagagtg tggccgagag agccggaaaa cacctcaaaa aaacaaccat ggagttgggc
    32461 gggagcgacg ccttcatcgt gctggaagac gcagatctgg acaaaaccgt gaagtgggcg
    32521 gtctgggcca aaatgaacaa caccggccaa tgctgcatcg ccgcaaaacg ttacattgtc
    32581 gtcgagaaac ttgccgaccg ttttttggaa aaattcaagt ccgcgctctc ggccttgaca
    32641 cccggaaacc cgatggacaa ggcgacgacg ctcggtccac tctctacgga aggggcactg
    32701 gttaacttgc tcaagcaggt cgatcaagcc gtcgcgcatg gggccgacct cgtcctgggc
    32761 ggcaaacgga tggatcggcc cggcgcgttt atggagccga cgatcctgac caacatcaag
    32821 cccgacaatc ccgcttaccg ggaggaattc ttcggccctg tcgcattgat gttccgggtc
    32881 aaaaacgaag acgaagcagt ggtgctcgcc aatgactcgg acttcggttt gggaggatcg
    32941 atttttacca acgacgtggc tcgaggtaaa cgcgtggcca gccgcattaa caccggcatg
    33001 gtcttcatca atcaccccac ctggacggcc ccggacctgc cctttggcgg catcatggat
    33061 tccggttatg ggcgcgagct ttccagcatg ggcatccagg agttcgtgaa caaaaagctg
    33121 gtgcgcgtgg aatccatcga cgccccggca taggaagcat tattcatgaa ggttcgtgac
    33181 gaaacggcgg agacgccatg cgagacaggg cgtggagccc cacgaggtcg agacgtactt
    33241 gacacagtac atagggagac cgcgcggggc gagcccgcct gcctggccgc gtaacagcca
    33301 cggccagact tgtcggagcg acgcatgcgc tgtagcgaat caagatcgat gattcggacc
    33361 gcgtcaagat ggttgatggg gattttaatg agtcctgagc ctaggccatc ggagtttgca
    33421 atttgctcgt ggctcaaacc ccttcgagca aacggttccc cgaccgatga acgattttga
    33481 gataggttct aaccacggga tggttacaaa acggtgcaat gacggcaacc ttaagaaagg
    33541 atcacatcat gcaaacacga aaactcggaa acagcggctt ggaagtctcc gccctcggtc
    33601 tcggctgcat ggggctgact tcgttctaca acacggcgtt gccggaaggc gaagccgtcg
    33661 ccttcctgcg tgcggcctat gagaaaggga ttactctctt cgacacagcg gaggcgtatg
    33721 gtcctttcgt caacgaaacc atcgtcggca aggcggtggc gccctttcgc gacaaggtcg
    33781 tgatcgccac gaagttcggc ttcgatatcg atctggaaac cggccggcat cttggcggtg
    33841 tcaacagcaa acccgcccat attcgcgccg tcgtcgacgc ctccctcaaa cgtctggcga
    33901 cggaccgcat tgatcttctc tatcagcacc gggtcgatcc cgaggtgccg atggaagacg
    33961 tggcgggcgc agtcagggac ctcatcgccg cagggaaagt gaagcatttc ggcctttccg
    34021 aagccggtcc cgaaagcatc cgccgggcgc acgcggttca gccggtcacc gcgcttgaaa
    34081 gcgagtattc gctctggcat cgcggacctg aggaaaagat tctcccgctc tgccaggaac
    34141 tgggaatcgg tttcatgccc ttcagtccgc tgggcgcggg ctttctcacc ggcaagattc
    34201 aagctgatac caggttcggc gagtcggact atcgcaacac ggaaccgcgc tttgcgcagg
    34261 aaaaccgcga ggccaatatg gctctcgtcg ctttggtgaa ggatgcggcc gcacgcaagg
    34321 gcgcaacgcc cgctcaggtg gccctggctt ggctactggc gaagaagccg tggatcgtcc
    34381 ccattccagg caccacgaaa ctgcatcgcc tggaagaaaa tattggtgcg gtggatgttg
    34441 ttctcagcgc ggacgacatc gccgagatgg aaacgacgct tgccgggctt accttgagag
    34501 gcgaacggct gtcggcagga gagttggcga tgacggggct gtaggagcaa tatgacacaa
    34561 accattggaa tcatcggcag cggaatgatc ggcggccagg tagcccgcct cgcaatcgcc
    34621 gcaggcttca acgtcgtgat ctgcaactcc cgcggaccag agacacttgc cgagcttgtc
    34681 tctaagcaag ggccaagcgc ccgggccgct acctttgcgg aggtgacgga ggcagccgat
    34741 ctcatcgtgc tcgccgttcc cttcgccggg tacgccaagc tttccgccga agcgttggcg
    34801 ggaaaaaccg tcatcgatac aatgaactac tatccggaac gtgatggcgt gatgccggag
    34861 gtcaagaccg acacaatcgc cacaagcgag ctcgtgcaaa agcacctgtc ccgctcgcgg
    34921 gtcgttcgtg cattgaacaa tatggacttt gttcgtttgc tgagccgcgc tcggccaaag
    34981 ggctcggcgt agcgcagtgc gctgccggtc tccagtgacc atgctgaagc taaggcagtc
    35041 gtaatcgatt ttctcgataa gattggctac gacgccgtgg acatggggcc gctgtccgaa
    35101 agctggcgca gcgagccgac catgcctgtc tacgtaaatc cttattggga cctggacgac
    35161 tctcacgccg tcagctcctt catggttgct ccaggacgcg tcgtgacccg gcaggaagtt
    35221 gccgcgcttc tcgggaaggc catccgtcac gacaagatgt tcggcttgct tggggccttt
    35281 gacgtgagaa ccaacaggtt gtcgtgatga atgttcaaaa atccgcgtag gctcacttta
    35341 tctttattat gaaacgacaa atggcccgct atgttctgat cgtgctttcg aacccgacgg
    35401 acgggaaaga ggaagagtac aagtcatccc gatccagaag gtcaacgaag cctacgacag
    35461 actgctgaag tccgatgtga agtaccggtt ctcgatcgac atggcgtcgc tcaaatccga
    35521 gtcaccggcg tctcgcgtgg gataaaccat gcaaaagcat aaattgagaa agcaacctgg
    35581 aaacgataag aaaggagatt gccatgaaaa caccgcttct gaaactcaac aacggcgtcg
    35641 agatgccggc gcttggcttg ggcgtctttc aatcctcgcc tgaggacacg atcaaggccg
    35701 tcgcgaccgc catcgcggat ggctatcgat tgatcgacac agcccgcgcc tacaagaacg
    35761 agagccaagt cggcgaaggc atccgcaaca gcggcatcaa ccgggcggaa ctcttcgtca
    35821 cgaccaagtt gtggctgacg gactacggct acgatgcggc gctgcgcggt ttcgacggaa
    35881 gcctgacgaa actcaggctg gactatctcg acctttacct gttgcactgg ccaacgaaga
    35941 attttgacgc aaccgtgcag tcctataagg ctgccgagaa gctgctggcc gcgggacggg
    36001 tacgggcgat cggtgtgtgc aatcacacgg agcagcagct cgaagcgctg atcgcgcgca
    36061 ccgatgtcgt tccagcggtc aaccaagtcg aactccatcc gtactttgcc cagaaggcgc
    36121 ttcgacagac aagcgcggcg ctcgggatcg tgatgcaggc gtggtcgccg atcggcgggg
    36181 tgaaacgcta ctggccggga gcgtccgcca ccggcaccgg cgatccgctt gcggatcccg
    36241 tcatcaccgg tatcggccgg gcgcatggga aaacggcggc gcaggtcatt ttgcgctggc
    36301 agttccagaa tggcgtcgtc gccatcccta agtcggtcaa agctgccagg atcgccaaaa
    36361 acatcgacat tttcgacttc gtgttgagcg actcggagat gacgaagatc gacgcgctcg
    36421 ataccggtgc gcgcggcggc cccgatccga cgcaaatcga tatggacagc ttctctgata
    36481 cagttgaccg gatgcacccg gttcgtgtca gcagacgcga cgcaaaacaa acggaaccgc
    36541 taaatgaaca tcgatcgatc aggcacagtt gctcttatca cgggtgccgg ctccggaatg
    36601 gggttggcat cagccgaggc attcttggaa gccggcgcgt tcgtcgtgct gtcaggaagc
    36661 cacaataaaa aggtcagcat tcaatcaaca ggagtctgca accatgaaca gagtactggt
    36721 catcacctac ccgaagaaag acgatcgaat cgcgagcgtc atggccgctg aatatgccat
    36781 gaccaattcc tggcgcgatc agggcatcat cgaacatctt ttcgccaaag agaatgacgg
    36841 cggcggggtg gttgttttca atgagacgag cgtggagaaa gtccaggcgc tcattgcgca
    36901 acttccgctg tttcctttct ttgagaaagt ggagtatttc ccgctcaaca agatctattg
    36961 agcttccgcc aggttcatct ttcaacacag gagaaggtta tgcaacgcgt cagcttcaag
    37021 aatcgcaaca tcatcatggc cggcagcttg tgccagccac gagatttcaa ggatggcgca
    37081 cgctacccgg cggtcgtcgt ggtgcatccc gcaggcggcg tgaaggaaca gaccgctggt
    37141 gagtatgcaa agcgcctggc cgcggaaggc tttgtcacgc tcgccttcga tacgtcctat
    37201 cagggcgaga gcggcggcga gccgcgttac ctggatgagc ccatgaaccg cgtgggcgac
    37261 atctacagcg cggtggatta cctcaccacc ttgcccttcg tcgatgccga gcgcatcggt
    37321 attctcggca tctgcgccgg tggtggctat tccgtcaagg cggcctcgct ggatcgccgc
    37381 atcaaggcgg ttgccaccgc cagcgcggcg aatgtgggca acgcggcacg caagggatgg
    37441 gatggcaaga gccctatgtc cgctttgccg ggcacgctcg aaatgctcgt caaccagcgc
    37501 acggcggaag ccgccggggc cgcgatagcc tacgcgccct acgtacctaa cgtcggtgaa
    37561 agcagtccct tccgcgatct gcgcgagggc tccgactatt acctgacgcc acgtggtcgg
    37621 catccgaacg cgcccaataa gatgctcatg acctcgctca tgggctggat cgcgttcgat
    37681 gccttcgacc tggtcgatcc gctgctgacc caacccctca tgatctgcgc cggcagcgaa
    37741 gccgattcgc tgtggcaaag ccaggaactc tacgcgaagg caccaggcag cagcaaggaa
    37801 ctcttcatca tcgaaagggc ttcgcacatg ggtctgtatg acggatccgg ccgtgaagcc
    37861 gtgctcggga agatcacgcc gttcttccgg gccaatctct gaaggaatac cccctgtgag
    37921 tgccagcacg ccaatgacaa gttctgcaac aagcgccggg caattgctgg cacggcagcg
    37981 caggcatgcg agtccggagc ttcttgccgc agccctgctt ctccttggca cggtcgcggc
    38041 cctgcagagc gagtactcgc tgtggtggcg cgagccggaa caggaattgc tgcctacgct
    38101 tgaggaactg agcatcgggt tcgtgccgtt cagcccgctc ggcaagggat acctcaccgg
    38161 gaagatcgac gagacgacga cgttcgacag caccgacttc cgaaacaccg tcccccggtt
    38221 cacggcggag aaccgcaagg cgaaccaggc gatggtcgag ctgctcggcc gcatcgccga
    38281 gcggaagaag gcgacaccgg cccaggtggc gatcgcgtgg ctgctctccc agaagccgtg
    38341 gatcgtcccg atccccggca ccaccaaact gcaccggctc gaagagaacg tcggaggggc
    38401 tgccgtcgaa ctgaccccgg atgacctccg cgatatcgcg gacgccgcgg caaacattgc
    38461 cgtccacggg gaacgatact ccgaggcggc gcagaagatg atcaaccgtt aaagaagcat
    38521 tcacgacaat cctgctgtgc gggcagggtc acacgcatct cggtcattga taaccgtcat
    38581 cggggtctgc gcaacgcgcg ccatcaaacg aaaggaaccc acatcatgaa atatcgcaag
    38641 ctcggcaata ccggcaccgt cgtatccacc ctggcgctcg gcacaatgta ttttggcgat
    38701 gagacggccg agaacgatgc gttcgccatt ctcgacgcct tcctcgatgc cggtggaaat
    38761 ctggtcgata catccgatgt ctacgtcggt accgtcgccg agcagatcgt cggccgttgg
    38821 ctggcggcac gtcctcgcga gataaccgat cgcgtcatag tggcgaccaa gggccggttc
    38881 ggtgatgatt ccgatcccaa tgcggtaggc ctgtcgcgcc gccatctcca ccgggcgctg
    38941 gatgcctcgc tgcgccgcct cgacgtggag acgatcgacc tgtatcagct gcatgcgtcc
    39001 gacatgcaca cgccggtcga ggagacgcta tcgttcctcg atgatgccgt ccgcgcaggc
    39061 aagatccact acgtgggcct gtcaaacttc accggctggc agcttcagct catggtgtcc
    39121 accgccaagg cgacgggggt gcagacgctg gtcagtctgc aacagcaata cagcctgctg
    39181 tcgcgcgagt gcgaatggga gatgaacccg gccgcgcggc ataacggctt gagcctactg
    39241 ccctggtcgc cgctggccgg aggtttcctg accggcaagt atcaacgtgg aggaaccccg
    39301 gcgtccgata gccgtgccgg ctccggcaaa gacctgtacc agtggatctc tgcggattat
    39361 gccggctccg atcaaaattg ggcgacgatc gaaaccgtca tccgcatcgc gaaagagatc
    39421 ggcgccaccc cggctcaggt cgcgctgagc tggatcgcca atcgtccggg cgtcgccgca
    39481 ccgatcttcg gcgcgcgcaa cctgcggcag cttgacgaga atcttgctgc ggccaaactc
    39541 acactggacg agaacgccac gaaggccctc gaagtggtca gtgcgcccag atcgaacggc
    39601 tatccttatg gcgcgttcgg cgccggtcag cgcaaccgcg acttgcggga tggaaacccg
    39661 gcaccaggtc ttcccgtcgc gggcggatca gaccatccgc tcggccgccc atcgctgacc
    39721 gtcacttctc gcgtcagcga ctgaactcga accctaggga gaaccccaat ggaccgaccc
    39781 cgtattttca tccatatgca catgtcactt gacggaaaaa tcgtggggtc ctatctgccc
    39841 acggacgttg ggatggcttc ccaaaggcag tactacaacc tcttccttgg gcccgaccgc
    39901 catttccgca atcacaaggg atggctgtct ggccgcacta ccagcgagga caacttcacg
    39961 cactatcgga agctgaagct ggatgaattc gctccgccaa tgccggaagg cgatttcatc
    40021 gccgtcgacg acgcgccgat gcattatttc tcagtggacc cgtccggcgt gctcgcctgg
    40081 gaaacgaact caatcaccta cttcgacacc accgcccatg tggttcagat cctttccgga
    40141 aaagccacca acgcctacaa agacttcctg cgccgcaaag gcgtttccta cacatcgccg
    40201 gcgaggacgc tctagatctg gaagaggctg taaagaagat cggctccgcc ttcggtgttg
    40261 acgagatcat gctttgtggc ggaggaggcc tgaactggtc tttcatccgg gcagacctgt
    40321 gcgacgaggt gagcatcgtc ctgacgccgg ctgcggatgg aacgaaggga gcgcagaccc
    40381 tgttcgaggc ggatgagcgc tacacccagc cactcccaac cgccttcaag ctgaaggaag
    40441 tgcaacctct ggatgatggc agcgtctggc tccgatacac ggtagagggg ccgatcaact
    40501 gagaggttct tccggattca ccacacaatg acgaacaaga accggtcaga tcgaccaatc
    40561 aaggaaactg aaatgaaaca aaagaatcca cagaacaacg cgagccaaag caacccggct
    40621 ccaatcagcc gccgcggttt cttgctgtcg gggggcgcgc tgggagccgc catgtgtata
    40681 acacctgcct tgggaaaacc ggcggccgga ccagccccga tctgcgatca gaaccgcacc
    40741 agctctcctt tgatcactca acgccgcaca ttgggctcag gaaagcacgc attgcaagtg
    40801 tcggccctcg gctttgggtg catgggaatg aactaccatc gcggcctcca tccggaccgg
    40861 gatgcgatga tcaagctggc ccgccaggcc acggaacgtg gcgtgaccct ctttgatact
    40921 gccgaggtgt atgggccgtt cgtcaacgaa gaactcgtgg gtgaggccct ggccccgttc
    40981 cgcgggcaag tcgtgatcac caccaagttc gggtggaaga tcgatccggg cagtcgcacg
    41041 tcgatcggcc tggacagtcg tccgcaacac atcaaggagg tcgccgaggc ctcgctcaag
    41101 cggctgaagg tcgatgccat tgacctgttc taccagcacc gggtcgatcc ggacgtgccg
    41161 attgaggacg tggcgggagc ggtgaaggag ctgatccaag agggcaaggt caaacacttc
    41221 ggcctctccg aagccggggc gaagacgatc cgccgggcac acgcggttca gccggtcacc
    41281 gcggtccaga gcgaatacca tctaatgtgg agagcacccg agaaggacgt tctgccggtg
    41341 tgtggagagc ttggaatcgg gtttgtcccg tacagccccg tgaaccgcgg ctttctcgga
    41401 ggcaacctga atgaatacac ccggttcgat tccggaagcg acaaccgcaa gacactgccc
    41461 cgcttcacgc cggaagcgat gagagccaat ctggcgatcg tggaggcgct caatcgattc
    41521 ggccgcactc ggggagtgac atccgcacag gtcgcgttgg cctggctgct tgccaaacaa
    41581 ccctggatcg tgcccattcc cggcactacc aagctggcac acctggaaga aaacctgaga
    41641 acggcggacc tgaaattgtc ggccgccgaa atgagtgaga tcgagggttc cgtttcgaag
    41701 attgagatcg tgggcgaccg ttatccggcg gagcaacaaa aactggtctc acccgattaa
    41761 tcccgaagct gtcacccatg gctatgcacc acgccgcacg tttctgaacc gtcgggctgc
    41821 gtagggagaa aatgatgaaa gaaaccaacg acgaagatag aacctaacaa tcaagacccg
    41881 taggtcactc acgacgtgac tttattattt ccgcagcgac tgctgtagcg agtgtggagc
    41941 tgcaaacctg gtgaacttac aaccagcaca gggagcgaac gccatgaaca agcctgaatt
    42001 cttcgtcacc cccggttacg gtgagcgcaa cctaaaactg ttccactatt cccaggcggt
    42061 caagatcggc aaccggctgg agctttcggg tcagggcggc tggaacgatg actgggagtt
    42121 ccccgagtcg atcacggatg agatcgctca ggcgtttcgc aacgtcgagc ggactttggc
    42181 tgtcgccggc gcgcgctggg agtacgtgat tcatgtgaac tcttaccacg tggggctcga
    42241 cggcgaccag gaagaaatca accaggccat gacggagcgg ttccgtcact acatgcccgg
    42301 ccacgctcca gtttggactt gcctgggagt cgctgccctt ggagatccga agatgcgggt
    42361 cgagatccgc gtgaccgcca tccttccaga atgaagggaa ggagccggat tgggcattct
    42421 cggcggctcg gtagacggac ctctgccgaa agcgggccgc cctcgacgcg agaagcagga
    42481 cgtacacccg ccacgaatcg cggcgggcac catttagacg aggaggtaca ccatgcgagg
    42541 agcagttctc tacggcccgc gcgatgtgcg cttcgaagag cgcgacgcgc caaagatcat
    42601 cgagccgacg gatgccatta tccgaatcgc gctcacctgc gtgtgcgggt cggacttgtg
    42661 gccgtatcgc gggctgcagc cgatcaccga acccacgccg atgggccacg aatactgtgg
    42721 catcgtcgag gagatcggcg gcgcggtgcg gtcggtcaag ccggggcagt ttgtcatcgg
    42781 ctcgttcgct acgtccgaca acacctgtcc ccactgtcag gccggctacc agtcgtcatg
    42841 cgtgcatcgc gagtggatgc ttcgggcgca agcgccggtg ctccgcgtgc cgctggcgga
    42901 cggaacgctg gtgccgacgc cgggcgtgcc gtcgaacgac ctgctcccga gcctactgac
    42961 agcatcggat gttctgggca ccggctggtt cgctgccgat gcagcgaacg tgacaccggg
    43021 atcaaccgtc gtggtcgtcg gtgatggcgc ggttgggctc ctgggcgtgc tttcggctaa
    43081 gcagatgggg gccgagcgga tcatcgcaat gagccgtcac gagaagcgac agcagctcgc
    43141 ccgcgagttt ggggcaaccg acattgtgac tgagcgcggt gacgagggca ctacccaaat
    43201 caaagagcta accaacggtg ttggtgctga ctcggtgctg gagtgcgtcg gcactcaaga
    43261 atcaatgatg caagcgattc ttgcgacccg tccgggtggc tccgtgggtt acgtcggcgt
    43321 gcctcacgga gtcctgctcg atggtgagaa gttgttctac tcacatgtcc acctccacgg
    43381 tggccctgcg ccggttcgtc gctacctgcc caagctgatc gacttggtgc tggacaggaa
    43441 aatcaatcca ggcaaggtgt tcgatctgac tcttccgctc gatcaggtgg ctgaaggcta
    43501 tcgggcgatg gacgagcgcc gcgcgatcaa ggcgctgctg cggccgtagt tcgaaacatg
    43561 gacggtagaa ggaacatgct tgcccgaaag gaacgctcat gaagattctt tgcctcgacc
    43621 gtcccgaccc ggtgcgacgc tggaaaaaca gcagcctcac ctgcacgatg aggcgcgcca
    43681 catctgggag tcgtacaaaa agggcgtgat tcgcgacatc tccttccgcc aggaccggct
    43741 cggcgtcgcc accaccctcg agaggaactg tgcaggatcg ctgaagccga tgccctgtac
    43801 atttcgcaac gatgtggaga aggcctttga gctgatgaga acgaaagcag acggcatgtt
    43861 gaagccgctc atcacgtctg cgtgaaagga ggctctatag ttgtccctgc tcataatatg
    43921 ttggaggcaa gagattgggc tagacgtgtc aggttgtttt gatctatggt gcagcaggaa
    43981 gaaacactta cggaggcagt agtccagaat tgcatcgcca cggagaagta accttgtcgg
    44041 ggagtgatgg ctcatgcata ccaaaacgtt cttttgcatc atcagcgtca tcttccttct
    44101 gtcctggaga tctaacgtgg aggccaagtc gttaccggtg gtccaggtcg ggatcgtgcg
    44161 cgacggaccc tcggagcttg ttcccgagct ccgtgatctc attcagaagg aaattctcga
    44221 gatcaccaga ggggaattcg aggtgcggtt ccccaatgat ttgcagtttg atggaggatg
    44281 gtcggtgcaa ggggtgaaac aggcgatcga tcgtgtgctg gctcagcccc gtgtcaatct
    44341 cgtcatcgca ctcgggattc tcgcttccca ggaggtcagt caaagaaggc aactacccaa
    44401 gcctgtgatc gcgccgttga tcctcgatcc gcacttgcag gggctgccgt tcaagaaggg
    44461 cacgagcggg atgaaaaacc tcagttacct cgtcaccgga aagggcttcg agcgtgatct
    44521 cgaggccttc aaagaggtgt ttccctttac ccggttaacc ctcttggcag atcgggtgtt
    44581 actggaggcc tttccgccag ttaaggaccg cgcgcgaaag atggctgaga cgtacggcgt
    44641 gaagcttacc gtggttccgg tcgagacctc tgccgaggcg gccattggct cgatccctcc
    44701 cgacacagag gccgtctatc tctcgcctct cccgcggctg ctcgccggtg aattcgatcg
    44761 tctggttttg gctctcaacc ggcgaaaact tcccagcttt tcggcagtgg gacagcggga
    44821 cgtacagcgc gggatcactc tcgctctgtc gcctgaattg gatctggggc gattcgcgcg
    44881 aacgatcgcg gcgaatgtgc agcgtattct gtcagggacc gacgccggac ggcttgaagt
    44941 ggcctttatc cggggcgagc agctcgtgat caacatggcg acagtccgtg cgatcgacaa
    45001 gtggccgagc ttccaagtgc tgagcgaggc agagttggtg aacgaggatg cagccggcac
    45061 ctcacgacgt ctgtcgctct tcgaagccgt tcgtgaatcc gcagaagcga acctggatct
    45121 ggccgcggcg agccgcaagg tcgcggcggg agtcggccag gtgggccagg cacgatccgg
    45181 tcttctgcct caggtgctcg tcggcacgtc aggggttctg aacgggcgag ggccggatac
    45241 gtttggcacc ggcgggaccc ccggtcaagc cgcgattgcc cttggcggat tccgccagct
    45301 cctgtactcg gacgacgcct ggaccaagta cacggtgcag cagaagacgc aactgtctcg
    45361 agaagaggag tggaaccagc tgcggctgga tatcgcacag gacgcagccc tcgcctatct
    45421 gaccgtcctt cggaccaaga ccgtgcgacg tattcagaag gataacctca aactgacgcg
    45481 tgccaatcta gacatggctg cggcccgtga gtcggtcggc tatgccctcc gggatgagct
    45541 gtttcgatgg gaagcagagg tggctaacgg gcagaaagcc gtggtttccg ccgaggctca
    45601 agaacggcag gcggaagtgg acctgaaccg aatcttgaat cgtcctctgg aagagaggtt
    45661 tcaaaccgtg gagcaaaccc tggaggaccc gggattgctg ggcgatacgc gtcagctgtt
    45721 cgcgtatatc gagaatccgc ggggatttca agtattccgg gacttcgaag tcgaagaagg
    45781 gatccaggcg gctcccgagt tgcgacggct tgacgccttg agtgcggctc aggagcgaac
    45841 catcacgaat gctcagcgag cctattggct cccagaagtt gccatacagg gtgccgggtt
    45901 tcagcaatat gcccaagggg gaggaggagc gggaagcctc acggtccccc ttggaccggt
    45961 cggctttgcg cagacccaga acaattttta tgtagcgagt atcgggttga cgttccccct
    46021 gttccagggc ggatacaaag atgccacggc cctcaaggcc cgtgaagaac tgcgtcaggt
    46081 gcagttcgag cgggccgccg tcgcacttca ggttgaagag cgggtccgga ctacgctgta
    46141 tcaaaccggc gcttcctttc ccagcatcag actctcgcgg aaagcggccg agtcggcccg
    46201 caagaccctg gatctggtcg tggatcaata ttcccgaggg gcggtggaca tcatcaagct
    46261 tctgaattcg cagaatgccg cgctgaccgc gaatcaggcc gccgccaatg cggtctatga
    46321 atttctgatc gatctggcga aggtacagcg ggcggtgggg cagatgagtt tctttcgaag
    46381 ctccgaagag cgagccgcct ggttcgaacg actgaaaacg cattttgtga atgccggcgt
    46441 ctcgccggtt ctgcgcgatg acccacggaa ctgattgaca cggagtgact ccatgccgac
    46501 cccaaagatc gctcgcccca ctctcatact aggagttggg cttctgttgc tcgtggcagg
    46561 agcctccgtc tggtggttct attttcgaat cccaccgctc gtgggattcg gcagcggcaa
    46621 cggacggctt gaggtgcagg agatcgatgt ggccaccaaa ttcccaggcc gcattgcgga
    46681 ggtgctggtt gatgagggcg atagcgtcca agtcggacaa gtcgtcgccc gcatggatac
    46741 gagttcgttg aacgcacagg tgcgagaagc ggaggcgcaa gtccagcgag ccaagcaagg
    46801 caagatcacg gccaaggccg tgatcgctca acggcggagt gaaaccttat tagccgaacg
    46861 ggatttggaa cgggctcgtg ctctctacgt caacgccaat atctccgtga aagactacga
    46921 tcgcgcgcgg tccacgatgg acacggcgaa gtccgcgatc acccaggccg aggcccaatt
    46981 ggccgaggcg gaagcggcca tcaaggcgag ccttgcccaa tcggaacgga ttcaggcgga
    47041 cctcaaggag agcgtattga ctgcacctcg gagcggccgg gtacagttcc gattggcgga
    47101 gcccggcgaa gtgctggcag caggaggaaa ggtcctgacg ctcatcgatc cgaccgatgt
    47161 ctacatgacg gtgtttcttc cggccgctga ggcggggaaa atagcgctgg gtgcccaagc
    47221 gcgaattacg ctcgatgccg ctccgaacct cgtcatcccc gctgcggtct cgtttgtcgc
    47281 cgacaaggcg cagttcaccc cgaaggaagt ggagaccagg tcggagcgcg agaaactgat
    47341 gtttcgaatc aaggtcaaaa tcgatcccga gttggtcaaa gggcatgagg cccaggtcaa
    47401 accaggtctc ccgggcgtgg cctacgtaca actcgattcg tccgttcagt ggcctccatc
    47461 cctgcaaacg aagctgatac gatgagtgaa ccatccgcat ccgtagcccg tgtcgccacg
    47521 ttgacccatc gttatgggga cacgactgcc cttgataccc tgaccttgga cattccggcc
    47581 ggccgcatgg tggggttcat cggtccggac ggagtcggta aatccagtct gttgggcctg
    47641 attgccggtg caaaaaaaat ccaggccggc cgggtcgaag tgctgggcgg cgatatggcg
    47701 gctgcgcggc atcgcaatga ggtctaccca cggatcgcct atatgcccca aggactggga
    47761 ggcaacctct atctgacctt atcggtcttc gagaacgtgg atttcttcgg ccgcctgttc
    47821 ggacagaacc gtgaggagcg cgaatggcgg atcacagacc tgctgcgaag cacggggttg
    47881 acgccgtttc gggaccgtcc cgcggggaag ttatcaggcg gcatgaagca gaaactcggc
    47941 ttatgttgct cgctgatcca cgatcccgat ctgttgattc tggatgaacc gaccaccggg
    48001 gttgatccgt tgtcgcgccg gcagttctgg gacctcgttg atcggatccg gaagcggcgc
    48061 gcggggatga gcgtgctcgt ggccaccgcc tacatggagg aggccgagcg attcgactgg
    48121 ctcgtggcca tggacgcggg caaggtgctt gcgacaggga gcccggcgga actgaagggt
    48181 cgcatgaacg tacggacgtt ggaagacgcc tttgtttcat tgctccccga agagaaacgt
    48241 cgaggccaca gcgccatcgc gatccctcct cgccggacac acgagggccc gccggccatc
    48301 gaagcgcatg gcctcacgat gcggttcggc agttttacgg cagtcgatca tgtgagcttt
    48361 cgcatcgagc ggggagaaat tttcggtttt ctcggctcga acggctgcgg caagaccacg
    48421 accatgaaga tgctcaccgg cctgttgccc gcatcggaag gagacgccag gctgttcgac
    48481 cggccggtga acgcccagga tatggagaca cgcaagcgtg tcggcttcat gtcccaggcg
    48541 ttctccctct atggtgaact cacggtgcgg caaaacctgg agctgcatgc gcgcctcttc
    48601 cacctccagc cggagaagat ccccgggcgt gtggctgagc tgaccaaacg gttcaagctg
    48661 gaagactatc tggatgatct ggcggaggct ctgccgctcg gaatccgcca acgtctctcc
    48721 ctggcggtcg ccgtcgtcca tcagccggag atattgatcc tggacgagcc gacgtccggt
    48781 gtcgacccgg tggcccgcga cggattttgg gagctcttga tcgagctctc ccgcaaagac
    48841 cgcgtgacga tcttcatctc cacccatttc atgaacgagg gggaacgttg cgaccgcatc
    48901 tcgctgatgc acgcggggcg agtgctggtg agcgatacgc ccgaaggctt gatccgtacc
    48961 cgtggcgctg cgaacctcga agaggccttc atcggctacc tcgaagaagc aagcggagag
    49021 gcgacgcaga aagaggtcgt cgaggaagcc gcagagacca tgccttccgc agcgggaacc
    49081 tcaagagctg atcgtggcag ggcgttcagc cccatgcggc tgttcgccta tgcccaccgc
    49141 gaagccctgg agctgagacg ggaccccatc cggctcacgt ttgcgctgct gggatcggtg
    49201 ctgctgatgt tcattcttgg gtatggcatt accatggatg tggaagacct gcgcttcgcc
    49261 gccctggacc gtgaccgaac gccgcagagc cgggactaca ttcagaatct cgccggctca
    49321 cgctacttca tcgagcgtcc gttcatcacc gatgatgcgg aactggaccg gcggatgcgc
    49381 agcgccgagt tgagcgtggc gatcgagatc ccttcgggtt tcggccggga tttgaagcga
    49441 gggcgccaga ccgaggtcgg cgtgtgggtc gacggcgcca gtccgttccg gggcgagacc
    49501 atcaaggggt acgtgcaggg catgcactat cagtacctcg ccgacctggt tcgtcgcacc
    49561 tacggcgctc agccacaggc cggcctcgcc gatctcgaga tgcgctaccg ctacaaccag
    49621 gacttcaaga gtctggaatc gatggttccg gcggtgattc cgctgttatt ggtcttcatt
    49681 ccggctctca tgacggccct cggcgtggtg cgggagaaag agctcggctc catcacgaat
    49741 ctgtatgtga cgccggtcac acgcctggaa tttctgttgg ggaaacaact accgtacatt
    49801 gccctcagca cgatcagctt cttcggtctc gtcgcgctgg ccgtggtcgc gttcaaggtt
    49861 cccctcaagg gcagctttgc caccctgacg ttgggaggag ttgtgtatgt gatcgtcacg
    49921 acgggaatcg gcttgctgat gtccgcgttc acccgtagcc agatcggcgc agtggccggg
    49981 acagcaatct tgacgatgct gcccacggtc cagttttctg ggttgaccga tccggtgtcc
    50041 tcgcttgagg ggatcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncga
    50161 ttggcatcgt cgacgaggat cagtcgccac tctcggctcg catcatcagc gctttttatg
    50221 gtccctattt caaaccaccc cgggtgatct cgatggatga gatcgaccgg ggcctggatg
    50281 ccggggaata cacatttgtc ctcgatatcc ctccggactt tcagagagac gtgctcgccg
    50341 gcaagcagcc ttcgatccag gtcaacatcg atgccacgcg gatgacgcag gccttcatcg
    50401 gcgcgaatta catccaaagc attgtgacgg gggaaatcac cgagtttgcc cagcgccatc
    50461 gaaccgggac cgtgctgccg attacgctcg cgacacgtgt gaagttcaat cccaacctga
    50521 acggcatctg gttcggcggc gtgatggaga tcatcagcca ggtgaccatg ctgtcgatca
    50581 tcctgaccgg cgccgccctg attcgtgagc gtgaacacgg cacactcgag cacctgctgg
    50641 tcatgccgct catcccgttt gaggtcatgg cggccaaggt gtgggcgaac ggcctcgtcg
    50701 tgctcgtctg taccgcgctg tccctgcggt tggtcgtaca aggagctttg ggcgttccca
    50761 tcgtcggttc cacccccttg tttttgttcg gcaccatgct ccatctgttt tccacgacct
    50821 cgatgggcat tctcatggct acggtcgccc gctccatgcc gcagttcgga ttgctgatga
    50881 ttatcgtggt cctgccgttg caaatgttgt ccggcggtat cacaccacgc gagagcatgc
    50941 cggagatcgt gcagaacatc atgctggcgg cgccgaccac gcattttacg gctttggcac
    51001 aggcgatcct gtaccggggc gccggcgtgg atgtggtgtg gcctcagttg atcgccatta
    51061 tgggtatagg aacggtgttt tttgtcggcg ctctgctgcg ctttcgggcg agcctggccg
    51121 cgcagagttg agggagaggt ggcctcgaca taccctgtgg gacttcgtac ctgccatggc
    51181 gctgctgggc ggttcgttgt tcggcttcga catgtggcgc tttgggcgcc agttccaatg
    51241 agtcgcgcga tgcgaggtgt ttcgaacatg agacaaggac gatgggctgt ggggatactg
    51301 gcgctggcgg tggccacggc ggggccggtt ctcgcggagg aaccgccggc cgggcaagcc
    51361 gtcgattgcc gttatgagta tgcgaccgaa ggcgtgaccg gcgcggcatt tcccacagac
    51421 gacgtctttc gacccttgat ggccgatccg aaacaaccgc agttctttgc cagctacctg
    51481 gctgcccgcg cgaggacgga tgggacccat gccaatatcg ggtcagtggg gttcggggaa
    51541 aacttcgggt tctatacgag gcgagagggt tgtaacggat ggcaagtcgg tcttctcgcc
    51601 ggggtgtttt cccaattcga cctgagcgcg ccctcgtcgg atctcatcaa caccgactat
    51661 gtcgtgggcg ttcccctgtc ctggcgatcc gggggctggt cgacgagagt ccgtctctat
    51721 caccaaagca gtcacctcgg cgatgagttc ctgctgggcc ggccggggtt caaccggttg
    51781 aatctgagtt ttgaagaact ggaagcgctg gtttcctacg actaccggtg ggggaggttg
    51841 tacgcgggcg gagggtattt ggtccatcgc gaaccggcct cgctcgaccg gaatcgcgtg
    51901 cagtggggag tcgagttgcg cggccccacc attccatcac cgatccttgg acgggcgctg
    51961 gcagggtttc tcgtcacgcc ggtgctcggg gcggacttca agtcattcga ggagttgaac
    52021 tgggtgatca atgccaatgt cgtcggcggg atcgagtggt cgagacaggg atcgagtcgc
    52081 cgcttccgct tcctggtgaa ttactatcac ggcttcaatc cctatgggca gttcttcgcg
    52141 cagaaagtcg agacggtggg atttgggttc tacctgcagc tgtgatcgag agccgctcgt
    52201 ccaaataagc gaacatgtat gagagaaaac gattgccgat atcacggacg atcggcgaaa
    52261 tggaataaga catgaatgcg ttggctcgtg cgctgtttat cctactcata ctcattgggg
    52321 cagtccaggg atgcggcctg tgggctgccg aaggcggtgc ctccaaagga aagcggctgt
    52381 tcgaggagca ctgtatggtg tgtcatggcc agggaggcaa gggggatggc tatcgtctgt
    52441 tcaatccacc accggccgac ctgacctccc ccgcggttca ggaaaaactt gacgccgcct
    52501 tgctcaggac cattcatgag gggcgacgga atacggcgat gggaaggtgg aagtacgtcc
    52561 tttccgatga ggaggcccgt gaagtgctcg cgtatctgcg gtctctctct caataaacca
    52621 gaggggaaga aagagaggaa ggagtcaggc ctatgagcag agcgtcgcaa agtccactga
    52681 gccctgagat gctgcggaag atagacgcgt actggcgcgc ggccaattat ctctccgtcg
    52741 gtcagattta cctgtacgac aatccgttgc tgaaacagcc gctgaagcgc acgcacatca
    52801 aaccgcggtt gctgggtcat tggggcacga ccccgggtct gaacctcctc tatgtgcatc
    52861 tcaaccgcct catcaaggaa caggacctga acgtcatcta tatcgccggc ccaggacatg
    52921 gcggaccggg gctggtggcc aacgcctacc ttgaagggac atatagcgag gtgtacccga
    52981 acatctcaca ggatgaggac ggcatgaagc ggctgttcac ccagttctcg ttccccggtg
    53041 gaattccgag tcacgtcgcg ccggaaaccc ccggctccat tcacgagggc ggtgagctgg
    53101 gttactccct ctcacatgcg tatggggccg cgttcgacaa tccggatttg atcgtcgccg
    53161 ctgtggtggg cgacggtgaa gcggaaaccg gtccgctcgc gacgagttgg cattccaaca
    53221 agtttctgaa tccggtcacc gacggggtcg tccttcccat tctccatcta aacggataca
    53281 agatcgcgaa tcccaccgtg ctggcccgta tcagtcacga cgaactggat cacctgtttc
    53341 gcgggtacgg ctacaccccc tatttcgtcg aagggcgtga tcccaggacg atgcatcagc
    53401 tcatggccgc cgcgctcgac gccgttctgg aggacatccg gcgcatcaag gcccgcgcgc
    53461 gccgaggggg cgcccacgca cgtccgctct ggcccatgat cgtgctccgg acgccgaaag
    53521 gctggaccgg tccgaaacag atcgacggca agcggaccga ggattactgg cgttctcacc
    53581 aagtcccgat gggggacatg gacaaaccgt cgcacatcaa gatcctcgaa aaatggatga
    53641 agagttacaa gccgaaggaa ctgttcgaca agaccggacg actcaagcca gaactcagcg
    53701 aactggctcc caagggaacg cggcggatga gcgcaaatcc gcacgcgaac ggcggactgc
    53761 tgctcaggga cttatgtctg ccggattttc gtgactatgc cgtgaaggtg acaaaaccgg
    53821 gtgcgatcgt ggccgagtcc acccgcctga tggggaaatt cctgcgggac acgatgaagc
    53881 tgaatatgga aggtcgaaac ttccgcctct tcagcccgga cgagaacaac tcgaaccgct
    53941 ggcaagacgt cttggaggtc accaaccgca cctggatggc ggatcgctat ccctacgacg
    54001 accacctggc gccggatggt cgggtgatgg agatcttgag cgaacaccag tgccagggat
    54061 ggctggaggg gtacctcctg accggccggc acgggttctt ctcctgctac gaagccttca
    54121 ttcacatcgt ggattcgatg ttcaatcaac atgccaagtg gctgaaggtc tgcaaccaca
    54181 ttccatggcg gcgaccgatc gcttcgctca attatctgtt gtcctcccat gtctggcggc
    54241 aggatcacaa tggattcagt caccaggacc ccgggttcat cgaccatgtc gtcaacaaga
    54301 aggcggaggt tgtccgggtc tatctccccc ccgacgccaa ctgcctgttg tcggtcaccg
    54361 atcactgcct ccgcagccgc aactacgtca acgtggtcgt cgcggggaag caaccggctc
    54421 cacagtggct tgcgatggac gccgccgtca aacattgctc cgccggtctc gggatctggg
    54481 aatgggccag caacgacagg ggaagcgagc ccgacgtggt gatggcctgt tgcggtgacg
    54541 tgccgacgct cgaaacactg gccgcaacag acctgctccg ccgccatctg ccgcaactga
    54601 aaattcgcgt catcaatgtg gtgaacctca tgaaactcca gccgccaaag gagcatcccc
    54661 atggattgag cgacaaagaa ttcgatgcgc tcttcacgac cgacaaaccg atcatcttcg
    54721 ccttccatgg atacccgtgg ctgattcaca gactgaccta tcggcggacc aatcacccgc
    54781 atttgcacgt gcgcggctac aaggaggaag ggacgaccac cacgcccttt gacatgtgcg
    54841 tgatgaacga cctcgaccgg ttccatctgg tcggggatgt gatcgatcgg ctgccgcagc
    54901 tcggatcacg ggcggcctac gccaaacagg cgatccgcga gaagctgctt gaacacaaac
    54961 agtacatcgc aacgcacggc gacgacatgc ctgaggtgcg tgattggaaa tggagcagaa
    55021 acgggtgatc caacagggtg aaagttttcc tctcggctcn nnnnnnnnnn nnnnnnnnnn
    55081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55141 nnnnnnnnnn nnnnnnnnng actgggaggg agatatcccg ctgaaacggc cgtttgccaa
    55201 gactgtgatc tatgagttgc acgtgcgcgg gtttacccgc catcccagtt ccggtctctc
    55261 tcacgaaacg cgcggtacgt atcgaggatt gatcgacaag attccgtacc tgcaggatct
    55321 cggcgtcacc gcagtggagt tgctgcccct gttccagttc gacgaacagg acgctccgag
    55381 gggtctcgtc aattactggg gctacagccc catctcattc ttcgtgccgc accacgggta
    55441 tagttcccgc aaagatcact tgggcccgat ggacgagttt cgcgacatgg tcaaggcgct
    55501 tcatcgggcc ggcatcgaga tcattctcga tgtcgtgttc aaccatacgg cggaaggaga
    55561 ccgcgccggc ccgacgctct gctatcgcgg cctggagaac gcagcgtatt acatgctgga
    55621 ggaggaccgg tcacgctatg ccgactatac cggcaccggg aacaccttga acgcgaatca
    55681 accgattgtc cgccggttga tcctcgacag cctgcgctac tgggtggagt cgatgcacgt
    55741 agacggcttt cgcttcgatc tggcctcgat cctctcccgc gacgaggatg gccggccgtt
    55801 gaagaatccc cccatcctgt gggacatcga atcggatccg tgcctagccg gaacgaagct
    55861 gatcgcggag gcctgggacg cggcgggcct gtaccaggtt ggctcgttta tcggcgatag
    55921 ctggaaagag tggaacggcc agttcaggga tgacgtccgc agcttcatga agggagacaa
    55981 cggcacggtg agccaggtgg cccgacgatt gttaggcagt cctgatctct atgggcacga
    56041 ggagcgagaa cccgagcaga gcctcaattt cgtgacctgt cacgacggat tcacgctgaa
    56101 cgacctggtg tcctacaacc acaaacacaa tgaagcgaac ggggaggaca accgggacgg
    56161 gaccgacttc aacctgagct ggaattgcgg agtggaaggt ccgaccgacg acccgtccat
    56221 cgagc
//
LOCUS       DAOQEW010000005        44669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_158_length_44669_cov_8.429193, whole genome shotgun sequence.
ACCESSION   DAOQEW010000005 DAOQEW010000000
VERSION     DAOQEW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 44669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44669
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_44669_cov_8.429193"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLS35_01230"
     CDS             complement(<1..272)
                     /locus_tag="PLS35_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HNI17296.1"
                     /translation="MGDWAKYGLYFLLGGTIVSISTYLGSQGRSFLAAFASTFPAMTG
                     ATFVLIYLNGGNEHLVTYAKNLLWFVPPWLVYVGCMIYGVERIGFWL"
     gene            complement(285..1304)
                     /locus_tag="PLS35_01235"
     CDS             complement(285..1304)
                     /locus_tag="PLS35_01235"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17297.1"
                     /translation="MNPSFSSYLVNDVFGDPGLFVEVRWSKRALLFDLGHNDALGPTR
                     LLRASDIFISHTHMDHFIGFDALLRVALGRGKTLRRHGPPGLIPNVQGKLHGYTWNLV
                     DGYPLTITAHEFHPDGIQTATFLATDGFQRHDEPDAPLNGCTGQGPFTVFRDPMFTVQ
                     ATALNHRIPSLAYALQEQFHVNFNKERLHEAGLPVGYWLKEVKQYFWEGQPDDFRFCA
                     TLYHEHHKETREFILGEIRERFVTITRGQKIAYVVDARYDEVNERKIIELARGADIFY
                     CEAPYLDRDADKARDRYHLTAKQAGIMAKKAEVRELVVFHFSPRYTGLGHEIEAEARE
                     AFRGQ"
     gene            complement(1392..2075)
                     /locus_tag="PLS35_01240"
     CDS             complement(1392..2075)
                     /locus_tag="PLS35_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17298.1"
                     /translation="MIRPEEASAAYPIQHNERGAALALLPLIATLLYYASPRELRQEP
                     LYQFLPQLCAYAALLTWSRLNGATVTRLGLTRALIPRGLRLGIATGLALGTANVLVIL
                     YLVPWLGADYRFLADTPHAKIPLLLMLPWFILMIAAMVELNFRGFLLGRLLGLGLPSA
                     LAVPLSALLFAFDPFLVATFHHLHWIAVWDGLIWGSLWVAFRNLYVTIVAHAVEVIVL
                     YSVMRTILT"
     gene            complement(2082..3632)
                     /locus_tag="PLS35_01245"
     CDS             complement(2082..3632)
                     /locus_tag="PLS35_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI17299.1"
                     /translation="MTNPMLRGCLLIGGATALLLWTASTSFGLEVTKPVPTERREAMS
                     LADAVLKALQNNLDIHIGRQTKESRLADIIIEQAKFDPTISLNGQYNRQVAPLNRPIL
                     GFTGANLQDITKFDQNTSSVTADITQNLPTGANYDLNYSPQRSYVSGPNTFLFNPAWT
                     GGLALTVTQPLLRNFGTDVNRTFISIAQNNATVEQHVFLDRVLTVIATVEQTFWEMVF
                     ANENLKVAQAALKAAEELLASNRAKAKAGVMSIVDVLQAEAAVASRVEQILVAEKSIR
                     DQEDQLRRLLNPAEEELRQDLRLMPTDPPTTSLEAISLQEAIDIAMERRPEVLQAGKN
                     VESSDLNVKFAKNQLLPTLSVQGTMGLSGLGADYGDATRRNFGGDFYNYGAGLVLSYP
                     IGNRSAYSTYNKRQLESRNAQASLQSVRQQVIVGVREAVRRVHTDFKRIETTRSARIM
                     AEKQLQAEQERLKVGLSTTRFVLDFQRDLATAQGNELRATVDYNKSLSNLARNKATTL
                     ERYSLRLE"
     gene            3681..4139
                     /locus_tag="PLS35_01250"
     CDS             3681..4139
                     /locus_tag="PLS35_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17300.1"
                     /translation="MMVAWYHKRRSVSMLARKGRATMRSTGIMLVAVTAALLWVSPAA
                     AQSVSGVRGFNGGVAPLFGLVGPGNLYIDNQGTQGFIYNFGNNFESYNFRNPTTGQAW
                     SGAVMTLGPQLSVGLIQGANQSGSPVVFPPVPRETAPLPPIQSGLLEDIP"
     gene            4691..5764
                     /locus_tag="PLS35_01255"
     CDS             4691..5764
                     /locus_tag="PLS35_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI17301.1"
                     /translation="MRIAQVSPLWESVPPKLYGGTERIVSYLTEELVRQGHEVTLFAS
                     GDSVTKAKLESPCQQALRLNTGIFNREAPLIQMMEQVFAAADQFDLIHSHLDFLAFSL
                     SRRCRVPVVTTLHGRLDLPELVPVFRDFAELPLVSISDSQRRPLPWCNWANTVYHGLP
                     KDLYKFNAEPGKYLAFLGRVSPEKCPDQAIELAKRVGLPMKMAAKVDPADRAYFERVV
                     EPLLDHPLIEFVGEITDAEKSDFIGNAIGLVCPYDWPEPFGLVLIESLACGTPVLAYR
                     RGSIPEIIGHGVTGFISENLDEMVSQVSKLGTIDRHRCRQVFDERFTAQRMTNDYVKI
                     YQQLIADAATLPGKAGQQHASNL"
     gene            5798..7078
                     /locus_tag="PLS35_01260"
     CDS             5798..7078
                     /locus_tag="PLS35_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI17302.1"
                     /translation="MADESQSTPESNVTGWRLLATRDFGCLWAGQVISQIGDGLNKVA
                     LLWFVYEMTGSALKMTAIGLLQTIPPLVFGPLIGVYLDYLPKKTVMIVVDLLRTLMVL
                     LIPLFYALDLLTLERLYVLVFLVSIVSTIFGPALASAVPLIVQRSQLTTANAFLQSTT
                     NIGVLLGPAMSGLGIALIGAQNVLYVDAATFLLSALFLFPIRVRETKSVKDLDVLGTP
                     VMQDMMVGFRFVFLQHRVVFALMITAVLYNLAISAFVFLLPVVAKELLQVGPMELGWL
                     WSALGIGMLAASIWLARMPQGTFQERIGKIGRSLTIGGLAVCALGLIQTPVLFSTFLL
                     IIIIGGSTSLFYPVVWAMLQEVTPEHLLGRVFTTFSVGGMASAMVGMAGFGWAADTLG
                     PAVSLIGIGLLLLLTAMVTMQVSRRGLVVTPAVA"
     gene            7280..8308
                     /locus_tag="PLS35_01265"
     CDS             7280..8308
                     /locus_tag="PLS35_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNI17303.1"
                     /translation="MPMRTIQTVCVAGILSFVVASGLGSVRSMASEPQPSETKPDVAP
                     GSGSKDAEGKIKEPDTKPKEPESKPKEPEAKPTEQKSKDSDTKAKEPEPKAKDGEAKP
                     KGQEPKAKESTTKPKESEHKAKEGESKSSKEPDQKSKEPEAKPKDAEIAAEHPCPSVP
                     QAADAKPQVEAPVPSGDSGTGERQKSEPEPKKPVRSSMVTAKLALMADPHLFPYDIEV
                     DAKDKDLVLLGKVGQESDKRVATDIIRCLEGVHAVENRLKVEADAAHGLFAERDKIIT
                     QLVKERFEKSKTLQSVKFDVKTEDGVVTLNGATRFQIIVLEAAQAARQVPGVRAVNTD
                     AVRLVAGE"
     gene            8364..9707
                     /locus_tag="PLS35_01270"
     CDS             8364..9707
                     /locus_tag="PLS35_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI17304.1"
                     /translation="MEPGRASGTGPGRPNVKWSPHLLTRDFTLVWWGQMVSQIGDGVS
                     KLALLWFVYSITGSPLKTTMIGLLQTLPPILFGPFIGVIVDRVPKKLLLISSDLIRAL
                     VLGVLPCLLPVDSFSIERLYLMVFVHAVASAVFGPALTAAIPSLVSRHEFTAANALLQ
                     TTTSIGIIVGPALSGVGIATMSSQEVLCVNAVSYVISALCFLLIRFPRTETVPAAEGG
                     SLAGTLRDVMEGFQYVLHRQRVILMLIGAASMYTFATSAFSTLFPVFGKKLLDLGPIE
                     VGYLWSAFGVGLLLVSLGLVSLSSWSLPKRIQLMAVSSFISGLTLLGLLFTSNRLVAA
                     VLMVIIGMGAGTLTPVAWGVLQEIAPTTLLGRVLAIYNLGAMTSAIAGMTIFGWVTQE
                     FGERPSVFGIGVGLFLSAVVSWRVARWVQVNWSETSVPAEEAARSVVMVTQPTSH"
     gene            9848..12013
                     /locus_tag="PLS35_01275"
     CDS             9848..12013
                     /locus_tag="PLS35_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNI17305.1"
                     /translation="MEEIISVNDQFYILASSSMADDRTRVLKHGETFGVFDRYGDIQP
                     VGRGTQGVFHQGTRFLSRQEIFLNNDRPMLLSSTVKEDNALLAVDLTNPDLYRDGRIA
                     IPRGSVHVFRSRFLWNGVSYERFRLSNYSLAPVKMTLSIRFEADFADIFEVRGKKRER
                     KGRMLPNILRKDLLVLRYEGLDEVTREARIQFVPEPLEITASQATFGIELEPKGEIAV
                     AVMVACDEVDSHRPLLSYDAAMAEAGLAFGAEQTEDCTIQTSNAQFNEWWNRSVLDVR
                     MMVTDTSEGPYPYAGVPWFSTPFGRDGVITAMECLWIRPELARGVLAYLASTQAKEVN
                     PAQDAEPGKILHETRKGEMAALHEIPFGLYYGSVDSTPLFVMLAGAYYERTADLPFIQ
                     SIWPNLEAALTWMDTYGDIDRDGFVEYVRKSPTGLDNQGWKDSHDSISHADGSLAEGP
                     IALCEVQGYVYDAKVQASKLAEALGYVDRASQLRRQARSLKERFEEAFWCEASSTYAL
                     ALDGHKRPCEVKTSNAGHCLYTGIASDEHARRVAETLMSDELFSGWGVRTLADSERRY
                     NPMSYHNGSIWPHDNAMIAVGLARYGLKAGVEKIMTGMFEVSLVLDFHRLPELFCGFV
                     RRPGQGLTRYPVACNPQAWAAGSAFMVLQACLGLSIVASEQKVIFNHPILPDFIDKMQ
                     IKNLTVGTASVDLLLRRHDLDVGITVIRRVGKVEVVSVK"
     gene            complement(12044..13585)
                     /locus_tag="PLS35_01280"
     CDS             complement(12044..13585)
                     /locus_tag="PLS35_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNI17306.1"
                     /translation="MSAQSHHSNLVGQLILSLWIALTPVTVLAENQLEVPQSLRQSLE
                     LVSSQRMVEDVRTLSGPTFNGRQTGTSDDLASAEFVRQRFLESNSSQPPLQESRSKGS
                     PAHASIRIQSTSHRPTIIGKDSFLRLAGSADSPPDQIGPDYLPVLDSPSADVQAPVVF
                     VGYGISDPESGFDEYAGLDVRQKVVLFLRGKPERYAKQVSHADKAHAAHAHGALAYLT
                     ATGPILNAYETRRGVTGRPSAFYGLTEPDRTIPGAWISTTRAMAILHTDRTGHEDRLR
                     TLQQHLNEGQPPQSIATDTVVTMRWHSAVQDGLLHNVVLTVPGSDQAKAEETIVIGAH
                     RDHFGRQGGLLFPGADDNASGTAVLLEIARVLQAAPVQPKRSVVLISFSGEEQGLVGS
                     KFYISQPTAPLRSTLGMINIDHAAVGNGRLTIGVTGMDKAAAQQAGQMAGVADRLDVF
                     GFFPGGDHVPFKEAGIPTVTVVSGGIHPHFHQPTDTAETINPDILSEAARYVLAMLWQ
                     LADAP"
     gene            complement(13627..14064)
                     /locus_tag="PLS35_01285"
     CDS             complement(13627..14064)
                     /locus_tag="PLS35_01285"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNI17307.1"
                     /translation="MDLRQHPPRRWSDATAGLIWLPRLIDKVRAFQAGTLGAYAYPSA
                     LDQSFMRHLRLTPAQIESLVRDMTSDEAIGAALRQHIPLSTEEIQERCAAFQKKYEWA
                     FAVLDRDDGYTRGLGYPLPQFLQRPLWRWYQRWSAQKAHATSI"
     gene            14260..14505
                     /locus_tag="PLS35_01290"
     CDS             14260..14505
                     /locus_tag="PLS35_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17308.1"
                     /translation="MAEQGKGLYDSQYARFRDNQGTHQGYEFQHGPAGSVPPPVVTFM
                     DKIKWWTLRGWRRKKILAERDRYQQDIIQIKTAHPKS"
     gene            14531..15361
                     /locus_tag="PLS35_01295"
     CDS             14531..15361
                     /locus_tag="PLS35_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17309.1"
                     /translation="MHYGYQRVRQIQVGRKNQWGGVTSFVRALRYVGVAAVVGVLAGG
                     METAWAVPKETSTEKAEHLRQQANPQLFNDWTFDKDEVGAIPKGFVAVTSGDQRPGSW
                     KVEAQAAVPSAPNVLLGTSGCGECQEILVAQGFQYEYPDLVVRIQQGGGAATGQVGVV
                     FGMKDAQNYYATVVDVSQKIIQVVRVIEGKESVLGRAAIKPKPVEWHTLRVQRNTIIS
                     KDFVETFFDGSLALSVEDQALGVGQVGVLVRGESSARFDNFNAAPLYSSRPLSAPAAY
                     "
     gene            15651..15947
                     /locus_tag="PLS35_01300"
     CDS             15651..15947
                     /locus_tag="PLS35_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNI17310.1"
                     /translation="MGSKIYVGGLPYSTTEQQLSDLFAVHGAVTSARIITDKFTGQSR
                     GFGFVEMSGDSEAQAAINALNGTQFGGRTLTVNEARPQEPRSGGGGRGMGGGRH"
     gene            16095..17444
                     /locus_tag="PLS35_01305"
     CDS             16095..17444
                     /locus_tag="PLS35_01305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI17311.1"
                     /translation="MVSFAELSLTSFLADRLQQAGFTAPTPIQSAAIPLALQGRDLLA
                     QAKTGSGKTLAFLIPLIERAVKEGWKPAGHASAQTGSSRSPRALVLAPTRELALQIEM
                     ELRKYAPPAVTSLAVYGGVPIERHYRALRQPPMIVIGTPGRLLDVAGTRHLDLRGIEY
                     VVMDEADQMLDRGFLRDIQRILQLLPSQRQTMLFSATFSPEILTLAESMLKNPARTAV
                     DPGVNTPTTITHAYYVVPSEASRVQLIHTLLQSSEAGDQSMVFCDQKYKVKRLAARLG
                     GEPASVGAITGNHSQAQRERTLTAFRSGRLRSLVATDVAARGLDVPTVSQVIHYELPG
                     NPTSYVHRTGRTGRAERSGATLLILSPQEEHEYLAMVRRLRIQTKRLTLPTLATLPPP
                     ARETESNSHPRHDGHGRDARPRRSGDRPNSTSQDARGGQGRRGWRAGRPAAPRRGNS"
     gene            17499..18032
                     /locus_tag="PLS35_01310"
     CDS             17499..18032
                     /locus_tag="PLS35_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNI17312.1"
                     /translation="MSSARNLLLSMMVAAGCLAAGEGAFAQSDLAIADGMKVSLEYTL
                     SLPDKSVADSNVGQEPITFVQGAHEIVPGLEKALDGMKAGQKRRIDVSAQDAYGPYNN
                     KLRQSIDKDKLPKDVKVGDILQASDNRLVKVLEVNDKKVLIDLNHPLAGKTLTFDVNI
                     LKVEKGDAADVPEKKNP"
     gene            18325..20322
                     /gene="uvrB"
                     /locus_tag="PLS35_01315"
     CDS             18325..20322
                     /gene="uvrB"
                     /locus_tag="PLS35_01315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNI17313.1"
                     /translation="MPPFKLDAPFKPCGDQGQAIDKLTSGILAGKQHQALLGVTGSGK
                     TFTMANVVERVQKPTLVLVHNKTLAGQLYQEFKQFFPHNAVEYFISYYDYYQPEAYIP
                     QSDTYIAKDASINDAIDQMRHAATTSLLQRNDVLIVSSVSCIYGLGSPEVYHDMLVYL
                     EEGMETRREKILAKLVDIQYARNDVDFHRGTFRARGDVIEIFPASSEAKSVRIELFGD
                     TVDAIHEIDPLTGKSLGKLPKIAIYPNTHYLIAPDRYERAITGIEEELEARVAAFRKN
                     GQLLEAQRIEQRTKFDLEMIRAMGYCHGIENYSRHLSGRAPGDPPPTLLDYFPKDFLL
                     IIDESHATVPQIGGMYEGDYSRKRTLVDYGFRLPSAVDNRPLKFAEFEQMLKQVIYVS
                     ATPGPYELEHAKGEVIEQIIRPTGLMDPLIDVRSAKGQVDNLLAEVRAEAAKGNRVLV
                     TTLTKRMAEDLTEYYHDLGVKVRYLHSDIKTLERAEIIRELRCGVFDVLVGINLLREG
                     LDLPEVGLVAILDADKEGFLRSHRSLIQTAGRAARNLDGRVIFYGDTVTDSMRSAMDE
                     TARRRHIQEAYNVANGITPESIKKLIPVLDYASGGTNAGQLELAAESLADYAPTADTE
                     QLIRRLEVEMKAAAKKLEFERAAELRNRIRSLKLKALEFTE"
     gene            complement(20365..20625)
                     /locus_tag="PLS35_01320"
     CDS             complement(20365..20625)
                     /locus_tag="PLS35_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HNI17314.1"
                     /translation="MRKSIGVLLIFILIGGMLGGIFGEILRVMAPNGAIQNIFASNFS
                     PGINPPLTVDLVLLKLTFGFSIKVNLLSVLGMFLGAYLYKQM"
     gene            21022..22371
                     /gene="ffh"
                     /locus_tag="PLS35_01325"
     CDS             21022..22371
                     /gene="ffh"
                     /locus_tag="PLS35_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNI17315.1"
                     /translation="MLDALSEKFERILKKLRGQGVLTEENIAEALKEVRLALLEADVN
                     FKVVKDFLDRVREKAIGQEVLKSLTPGHQVVKVVWDELRGMMGGERSGISLASRPPTI
                     LMMVGLQGAGKTTTSGKLARLFKSQGKRVLLVAADPRRPAAGDQLASLGHDLGIDVHR
                     FDQVDASRADVVRICQRGVERAQEQGYDLVVLDTGGRLHVDDELMAELVAVKEAVTPH
                     EVLLVADAMTGQDAVNMAGQFDRQVGLTGVILTKVEGDARGGAVLSIRAVTGKPIKFL
                     GMGEKLDALEPFHPDRMASRILGMGDVLSLIEKAQETFSREEAEAAQKKLTSSTFTLE
                     DFRSQLGQMNRLGSFEQILGMLPGGQKLKDLANSGLPEKEMKRVAAMIDSMTSRERRD
                     HTLINGSRKKRIARGSGTSVQEVNRLIKQFLQARKIAKVMSGGAGGRRQLAQMLRGM"
     gene            22472..22780
                     /gene="rpsP"
                     /locus_tag="PLS35_01330"
     CDS             22472..22780
                     /gene="rpsP"
                     /locus_tag="PLS35_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNI17316.1"
                     /translation="MAVHLRLARTGRHKRPMYRVIAADSRKPRDGRFLEILGIFDPLK
                     NPAVPVLKSERVLSWLRHGAQPTTTVRTLLKRHGVWKEFETEKSAKAEKPGKDEKSKK
                     "
     gene            22790..23317
                     /gene="rimM"
                     /locus_tag="PLS35_01335"
     CDS             22790..23317
                     /gene="rimM"
                     /locus_tag="PLS35_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNI17317.1"
                     /translation="MTPLDQAELVTIGRIERSFGVRGEARVRSLSDVPGRFEALREVT
                     IVASSGKSLVTRVTHVRSGGPTLIVGFEAFTTPEQVAEFRGGLLQVPRGDSPALPSDQ
                     YYQCDLIGMVVQDEAGTVLGQLEEVLAMSDNQAFLVRQDGKELLIPAAKQIVLSVDVG
                     KRVMTVRLPEGFGDL"
     gene            23319..24092
                     /gene="trmD"
                     /locus_tag="PLS35_01340"
     CDS             23319..24092
                     /gene="trmD"
                     /locus_tag="PLS35_01340"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNI17318.1"
                     /translation="MRCAVLTLFPDMVAPVLGQSILKRAQEKGLLEVSVQNLRDYTFD
                     RHKTADDVPYGGGAGMVMKAEPVLQAVDALRARYQASHPETALRVIMPSPQGRPFTQA
                     LAQSLAKDDRVLVFVCGHYEGMDERVNVALCPEQISVGDYVLTGGELAAMVMIDAAAR
                     LVPGVLGDAASAAEESFTEGLLEYPHYTRPAEVRGMAVPEVLVSGHHEVIRLWRRKEA
                     LRNTYLKRPDLLRDRELGLEDQRLLSEVMQESLVQVPVR"
     gene            24108..24512
                     /gene="rplS"
                     /locus_tag="PLS35_01345"
     CDS             24108..24512
                     /gene="rplS"
                     /locus_tag="PLS35_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNI17319.1"
                     /translation="MNRLERIQRSLTKKTAPKFEIGDTVRVHVKVVEGEKERIQVYEG
                     TVIARKGTLNSETFTVRKLSYNVGVERTFPIHSPSVAKIDVVRQGRVRRAKLYYLRTK
                     KGKFAKVEDREFKVESKAQAAAKVEAAAAAKA"
     gene            24602..25240
                     /locus_tag="PLS35_01350"
     CDS             24602..25240
                     /locus_tag="PLS35_01350"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNI17320.1"
                     /translation="MEARSRGYRHIAGLDEAGRGPLAGPVVAAAVLLPRRCRLPGLND
                     SKQIIEADRVRLFDEIVRRAIGVGVGAATEVEIDRLNILQASLLAMRRALMALPLQPD
                     FLLLDAVTLSGLSIPQRPIIKGDGLSCSIAAASIVAKVTRDRLMVEYHRWYPQYNFAE
                     HKGYATPEHLRLLREHGPCAIHRRSFAPVQHCFAEDDTVSLSLSDESESLFD"
     gene            25240..25626
                     /locus_tag="PLS35_01355"
     CDS             25240..25626
                     /locus_tag="PLS35_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNI17321.1"
                     /translation="MRDRRRLLGDEGEGQAEAYLRRQGYRILGRNVRSPLGELDLIAD
                     DAGTLVFVEVKRRRSGAYGGAIEAVDARKRAKLIQLASHYLAQHRLHDRACRFDVVLI
                     QDDAGSGAAVQHIANAFDVAGDDLRW"
     gene            25728..26396
                     /gene="ftsE"
                     /locus_tag="PLS35_01360"
     CDS             25728..26396
                     /gene="ftsE"
                     /locus_tag="PLS35_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNI17322.1"
                     /translation="MIQLFHVSKYYDRRPALSDVTLEIEKGEFVLLMGPSGAGKSTLL
                     KLLIGAERPEEGQILVQGRSLSKLRPSEIPVLRRKVGIVLQDFRLLPKKTVFENVSLP
                     LLVQGASTTEIRRKVAEALRAVGLDHKKDLFPPGLSTGEQQRICIARAIVNGPIMLLA
                     DEPTGNLDPELTSEIIELFKSINARGTTIIVATHDPNVLAQVNRRVITLEHGKVVSRD
                     QGAS"
     gene            26393..27292
                     /locus_tag="PLS35_01365"
     CDS             26393..27292
                     /locus_tag="PLS35_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNI17323.1"
                     /translation="MRRLLYLLREAVANVLTNRTTTLVAVATTAFTFACVGVFLLLYV
                     NLRTMASSLEQDIQVMVYLQDDLTEQARSEIEQQLKIDRAIASLTFVSKERALADFQA
                     QFPSESRLLQGLGQNPLPASFVVLMAPESRSSDAMRRWANRTQLIPGVSQVQYNQEWV
                     EALAGIVRYIEVAAIIVGVILSAASVTIIANTIRLALYSRREEIEILGLIGASTTFIR
                     VPYLLEGAALGLCGSALSLVILKGGFELFRHQIHSATRFLGVDALLTFFSFDMCLVLM
                     LVGLFLGCAGSFLSLLRFGEGRA"
     gene            27289..28485
                     /locus_tag="PLS35_01370"
     CDS             27289..28485
                     /locus_tag="PLS35_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNI17324.1"
                     /translation="MTRIQWVWVVLSVHLALSTSPPPAHAERKDSYADKIEQEKKTLE
                     KLRGTIVEKRKKADEAEKKRESILQGLQSLDERLVRYRQEHQDTVRKLKKKDVEIEQM
                     SVQLSRLSERIDERQDAIAARLRVQYVEGRFWQLKTLLAASSSGDFQRRFRYLSAVTQ
                     REYEIVEGYRKDAERIAEVERSREEARQGILLYKISTEEKLAQIQGLKKQKRVYLAKI
                     TQEKESHDRAVEELERSASRVDSLLKELETRRRAALANRPPSAGAGLRALRGTLLWPT
                     DGQVVSYFGRQKHPTFNTYVQRKGIEIRAAEGSIIRSVLAGQVVYADWLKGYGLVIIM
                     DHANGVFSLYAHASKILTSVGARIEAGNAIGETGDTGMTGENTLYFELREGAEPVDPL
                     VWLSKR"
     gene            28510..29856
                     /locus_tag="PLS35_01375"
     CDS             28510..29856
                     /locus_tag="PLS35_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNI17325.1"
                     /translation="MEQRRSRRWLYLLLMVTVALGVGIVLEKGLERTGHASETYEELR
                     TFSEVLTQVQKHYVDDTKVKDLVQGAIRGMLSTLDPHSAYMTPEMYKEMQVETKGEFG
                     GVGIQIGVKENRLAVISPIEGTPAHRAGIKAGDFITKVNDEPTKDLTLMDAVQKMRGP
                     KGTKVNLTIQRDGTADPLAFSLVRDTIKIESVKFKVLDNTIGYVRLTQFQEATGRDLA
                     RALKQFKEQKVQGTILDLRNNPGGLLTAAVDVSEQFVGNGKLIVYTKGREGKKDEWFS
                     KTKETLEDSPMIILVNEGSASASEIVAGALQDWGRAVIVGTTSFGKGSVQTILPLGDG
                     SGLRLTTAKYYTPKGRSIQSTGITPDIVVKLQTQTVAKAGDKDAKEAEPKTAKAPASG
                     KDSASSKPADEPAHKNGAASLNDAGGDISVEDDVQLQKAVELLKTWKIFKELRPAA"
     gene            complement(29831..30322)
                     /locus_tag="PLS35_01380"
     CDS             complement(29831..30322)
                     /locus_tag="PLS35_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNI17326.1"
                     /translation="MDDRLNQLAQIFRAMANLLAAQRANPYRVRAYRKAADAILMITG
                     DLTDLAARRQLQEIPGIGKDLAGKIEEFLTTGTIRAYEELRTPLPKEVAEWASLPGLS
                     DALVSYLYFRLNIRSLADLESLVASHLLRTQPGFSGSEDDLLAAIRRQRASDALTPPV
                     EAP"
     gene            30454..30660
                     /gene="thiS"
                     /locus_tag="PLS35_01385"
     CDS             30454..30660
                     /gene="thiS"
                     /locus_tag="PLS35_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNI17327.1"
                     /translation="MTIQVNGESRGIGDGHTVAALLRDLDIRADRVAVELNLEILDRK
                     DFETRGLCEGDRVEILSFIGGGAE"
     gene            30672..31442
                     /locus_tag="PLS35_01390"
     CDS             30672..31442
                     /locus_tag="PLS35_01390"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNI17328.1"
                     /translation="MNDRLVIAGREFQSRLWVGTGKYKDFAETKKAIDASGADVVTVA
                     VRRVNITDRSKENLLDYLDPKKYIILPNTAGCYTVEDAVRYARLARAAGVSDLVKLEV
                     IGDEKTLFPDTAGLIEAAKILIKEGFIVLPYTNDDPIVAKKLVDIGCPAVMPLAAPIG
                     SGLGIRNPYNLKIIMETVKVPIIVDAGVGTASDAALAMEYGADAVLMNTAIAGAQDPI
                     AMAEAMKYGVWAGRLAYKAGRIPRKLYATASSPIEGML"
     gene            31552..32178
                     /gene="thiE"
                     /locus_tag="PLS35_01395"
     CDS             31552..32178
                     /gene="thiE"
                     /locus_tag="PLS35_01395"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNI17329.1"
                     /translation="MSAVDFRLYLVTDRHQTAGRPLGSVLARAVRAGVRAIQLRERDL
                     PVRDLHALATELQRDLPDARLFINDRVDVALALRTVGVHLRESSLPAGVARCLLGPSQ
                     LLGTSVHSLDGVIVAEQQGADFVVLGPIYDTPSKRVYGPPLGMRILEQAAQKVRIPIF
                     AIGGITAARTREVRQAGAFGVAVLSSILSAVNVEQATEELLAAIQMES"
     gene            32428..34104
                     /gene="arc"
                     /locus_tag="PLS35_01400"
     CDS             32428..34104
                     /gene="arc"
                     /locus_tag="PLS35_01400"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HNI17330.1"
                     /translation="MTHKNDEHAREVEKLRTQIQSMEEEIRRLYQSRYQLEQTTKQNE
                     KLVATLQEAKSQIETLRAEVEKLTAPPSTYGIFSSLDTDGTGNVYVSGRKMKVSLHPS
                     IKAKELRKGQEVILNEALNVIAVRGFDVQGEVVRLKDVLEGNRALVTLHFDEEKVAEL
                     GEPLLSERLSVGDHLLYDPRSGCVIEKLPKSEAEELVLEEVPDVDYEHIGGLQREIEQ
                     VRDAVELPFLYPHIFSNYKLSAPKGVLLYGPPGCGKTLIAKAVASSIAKKLGHLKDRQ
                     LRSYFLHVKGPELLNKYVGESERQVREVFKKAKERADDGHPVIVFFDEMDALFRTRGT
                     GISSDIESTIVPQFLSEIDGVERLTNVIVIGASNRQDLIDPAVLRAGRLDVKVKVGRP
                     DATAARDIFSKYVSTDLPFAEEDLARHGGDARALVDSLMNLTVDAMYATTDENKFIEV
                     TYANGEKEVLYFKDFASGALIEGIVSRAKKFAVKRAIAQEGSGLRADDLIRAIREEFK
                     EHEDLPNTTNPDDWAKVAGKKGEKIVHLRTISGGPSEARQIETVSTGHYL"
     gene            34119..35618
                     /gene="dop"
                     /locus_tag="PLS35_01405"
     CDS             34119..35618
                     /gene="dop"
                     /locus_tag="PLS35_01405"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HNI17331.1"
                     /translation="MNDTHSHNLSRVIGTETEFGIASRDPNAADPVANSIHVIGYYPN
                     LPAPQAVWDYENENPLLDARGFEVDGERERPGPDYNRQLNKVLANGGRLYVDGAHPEY
                     STPECTNAREVVAFERVGERIIAQALAGITEARGRDQFVLYKNNSDGKGNSYGYHEDY
                     LVSRAVSFDRITQVLTPFLVTRPIYAGSGKVGAENQTPSVDYQISQRADFFETLVDLN
                     TMVRRPIINTRDEPHSDSTKYRRLHVIVGDANMAELSTYLKVGALSIVLDLLEAGADL
                     PRISLADPVNAIKQVSRDTQVKESLKLTDGSATTAIAVQRAYLKAAQSYYACHELNQV
                     TKDILVRWEDVLDRLERDPRLLVRELDWVAKRYLIESYMDRKSCGWDDPRVRLMDLQY
                     HDVRPDRGLYYTLERSHMIERVVLEHEIVRAETNPPVGTRAYFRGQCVKKYPNAVYGV
                     SWTSVLFDVGQNKIKRIPLMDPLRGTEALTGELFAHAETAAALLEKLST"
     gene            35632..36456
                     /gene="prcB"
                     /locus_tag="PLS35_01410"
     CDS             35632..36456
                     /gene="prcB"
                     /locus_tag="PLS35_01410"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HNI17332.1"
                     /translation="MNRMPFLPHHDGSSFFDFLTQYHPELRPGVHDLAMPRATLPLDL
                     GRPGAIPVPHGTTVLALKYRDGAIIAGDRRATEGFQIADRRIEKVFRIDEYSAMAIAG
                     AAGPCIEMAKLFQTELEHYEKLEGVQLSCEGKANKLGQMVKANLPMVFQGLVVMPLYV
                     GYDVKRKEGRIFKYDITGGRYEESDHHSIGSGGKDARNTMREHYRSGLDEEAALRVGL
                     LALYNAADEDVGTGGPDLVRGIYPTAKVVSDAGITDVPESRVRALYEAMIAERRRS"
     gene            36460..37143
                     /gene="prcA"
                     /locus_tag="PLS35_01415"
     CDS             36460..37143
                     /gene="prcA"
                     /locus_tag="PLS35_01415"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNI17333.1"
                     /translation="MPLPYYVSPEQMMQDKAEYAKKGIAKGRSIIALEYVDGIVLAAD
                     NPSSSLHKVSEVYDRIAFAGAGKYSEFEHLRKAGIRHADLTGYMYSREDVSARTLSNA
                     YSQSLGTAFSTDVKPLEVEILVVQVGGNGQANEIFRISFDGSIVDEKSFAVIGGRSEA
                     VQQYLREHATPQPPTLKDGLRRCLSALEQVATQKIPSENLEVAVLDRNRQGRKFRRLT
                     SADISHLLA"
     gene            37297..38673
                     /gene="pafA"
                     /locus_tag="PLS35_01420"
     CDS             37297..38673
                     /gene="pafA"
                     /locus_tag="PLS35_01420"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HNI17334.1"
                     /translation="MQQRIFGLENEYGLIFSPNGKIYLPMEKVLGYIFEGLIPNSWPS
                     NAFLVNGARFYQDTGCHPEYSTPECDNILDLVIHDKAGERLLEACLPAAEERLREEGL
                     SGEIYIFKNNTDSLGNTYGCHENYLMRRDVDFWKVTEQLIPFFVTRQIYSGAGKVLKV
                     SGKPQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIIVGDSNMSEY
                     ATYLKVGTATLVLSMIESGFSVTGMELEDPVKAIREISRDPTLNKKVKLDDGRQMTAI
                     EIQRVYVKRATEFLAAQDHDPVLDDVLDKWISVLDRLEDDPMQLVHEVDWVMKKHLIQ
                     SYTDKKDCGWDDPRVFLLDLQFHDVKRTRGLYYLMENRGMAARVVEEEAVQRAMSVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFSAVNRRVEELLSQ
                     VSGARLYR"
     gene            38706..39689
                     /locus_tag="PLS35_01425"
     CDS             38706..39689
                     /locus_tag="PLS35_01425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNI17335.1"
                     /translation="MSFLDRFLIISVVLLASVFPVELFPNTGPVPRGGDGQFSGKQGQ
                     VVVVTVPELKEATSVKGKFLGRTVTLFPNPTAGGTGYIGLLGIDLQDEPGPHELTIDA
                     QLGEQTRHFSYQVLVVKEKFAVEHLKLPKDKVDLDEKATARWKAEQEQVRKALAEESA
                     MRLWQAGFIEPVHGKRTGIFGSVRIMNGQPRNPHNGEDIGAPMGTDVMASNDGVVRLV
                     VDHIFSGRGIFVDHGLGLYSMYFHLSEALVNEGDLVKAGQVIGKVGATGRATGPHLHW
                     GMKVNGARVNPYALLELPFPQNPAADRPVLAAPAVPAQEELAPTAVGGDGR"
     gene            complement(39739..42195)
                     /locus_tag="PLS35_01430"
     CDS             complement(39739..42195)
                     /locus_tag="PLS35_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17336.1"
                     /translation="MQVLLIEDNEDDAALIREALCEHSPQDVLLTWADRLEAGLLKLA
                     EGSPDAVLVDLSLPDSQGMDTLVRVRAQAHEAAVIVLTGLDNDLVAEKSLLHGAQDYL
                     VKGRLTGDGLKRAIRYAMGRHRVEQALRKSEERFHLTCLATRDGIWDWDITSGETWFN
                     DAYQTVYGSGAKAREAGRIAWADQIHPEDRQSILAGLSHTLESDKNLWTAEYRFRRAD
                     GTYAYVIDHGYVLRNYAGTPYRMIGAKTDISDRRQAETMHAVQLTVGLALEESVSLNE
                     AVPKILRAMCELQGWTLGALWLVDTQHSVLRCNALWHPSGQQTEHFAQAYRTLFLRQD
                     MGMAGRAWKTDAPAFSPDIVNEADFPAHDAAREADLHGAIAFPIHTGKEILGIMEFFT
                     VEVLHPAVSRMERIAELGAMISQFLNRKDLERQLRQAQKMEALGRMAGGVAHDFNNLL
                     TIINSWAELLMDEPGLTSRAQRGMAQIREAGNKATGLTRQLLAFTRHQVVERQSLNLN
                     ERVSDIVELMKRVIGEDIHLVLTLDPTLGRIKADPGQIEQVVMNLVVNARDAMPHGGR
                     LELETGEVMISHSDPFWPDPLPPGPYVTLSVRDTGCGMSAETLAHVFEPFFTTKELGK
                     GTGLGLSTVYGIVRQSSGTVGITSEVGKGTSFTIYLPRLEEDTGAPRAVTQPKVTSEQ
                     SETILLVEDNDMVRGLAHTVLVAQHYHVLAARSGEEALEMVRRQGRHIALLVTDMVMP
                     GMGGAQLAHELQTLQPDIKVILTSGYSGRDETLLKTFDAQVAFLPKPYTPESLTKTVA
                     SALESQTRQGSCRDTSSHST"
     gene            complement(42199..42639)
                     /locus_tag="PLS35_01435"
     CDS             complement(42199..42639)
                     /locus_tag="PLS35_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17337.1"
                     /translation="MTMRPFDILIAEDNEDDILLIREAFETVNMTNRVAVVRDGAEAM
                     AYLRGEGSFSQCPLPGMVLLDINMPKKTGLEVLEEIKSDPRFRAIPVVILTVSEREED
                     IVRSFEKGACSYIRKPVTFTRFIAVVKEFELYWTLVSKVPTLKR"
     gene            complement(42636..>44669)
                     /locus_tag="PLS35_01440"
     CDS             complement(42636..>44669)
                     /locus_tag="PLS35_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17338.1"
                     /translation="ATAVTDSDGRFVSSRSTLIDITERKRADQALRLSHQSLEALVEE
                     RTAALQEANSRLEQELAQSKQMHQALIASETRFRELVESLPLPIWTCLPDGTCDYLSP
                     RWANYTGRSADEPFSTGCLQHVHPEDRPRITEQWHHSVAHIQPLDTELRFKRADGIYR
                     WFHAHATPIRDHDGHLLKWVGTYTDIHDRKQAQEIQARLASIVESSSDAVLSKGLDGR
                     ILSWNKSAELMFGYPSEEIIGQPVSRILPPERRAEEAILIEQITAGIRIQQFESVRLH
                     KDGHPIQVSLTVSPIVDANGEVTAVSTIARDITERKRAEEVLSQQRRLIELSYDPIFT
                     WDFERGILDWNRGCEQLYGYTRAEAIGRSSHTLLKITYPCPFDTICAELEATGAWTGE
                     LHHRTKDGREVLVESRWGLMKTGGRSLVLETNRDITERRQAELMILKKNKDLETLLYV
                     TSHDLKEPLRAIESFSVLLQERYASRLDEKGQDFLRRIVRATQRLDQLLTDILDLSRA
                     QRMEPPVDEIDAHQLVQEVLRRLESRIKESGATITVFTPLPRLRVNTTWAVQGIYNLV
                     ANALKFVADGRPPDIEIAPHTEIDAEGTECSGLVIRDRGPGVPSDQRERIFELFRRAV
                     GREIEGTGAGLAIVRQVAERHGGRAWVLPREGGGSDFFITFGINRQVVKEVGL"
BASE COUNT         9306 a        11997 c        13721 g         9645 t
ORIGIN      
        1 agccagaagc cgatccgttc gacgccgtag atcatgcagc cgacatagac cagccagggc
       61 ggtacaaacc agagcaggtt cttggcgtag gtgacgagat gctcgttgcc gccgttgaga
      121 taaatgagga cgaaggtcgc cccggtcatc gcaggaaagg tgctggcgaa ggcggcgaga
      181 aacgaccggc cttgtgagcc gaggtaggtc gagatactga cgattgtgcc gcccaataag
      241 aagtacagtc cgtacttcgc ccagtcaccc atccgcaccc tccttcactg tccgcgaaag
      301 gcctctcgcg cctccgcttc gatctcatgc cctaggcctg tgtaccgcgg cgaaaagtga
      361 aacacgacca gctcgcgcac ctcggctttc tttgccatga tgccggcctg cttggccgtg
      421 aggtggtacc ggtcccgagc cttgtccgca tcccgatcca gatagggggc ctcacagtag
      481 aagatgtccg ccccgcgtgc aagctctatg atctttcgtt cgttgacttc atcgtagcga
      541 gcatccacga cataggcgat tttctggccg cgcgtaatcg tgacgaatcg ctcgcggatc
      601 tcgcccagga tgaactcgcg cgtttcttta tggtgttcgt ggtagagcgt ggcgcagaat
      661 cgaaaatcgt ccggctgacc ctcccagaaa tattgcttca cctctttcag ccagtaaccc
      721 accggaagcc cggcttcatg taatcgctcc ttgttgaagt tcacatgaaa ctgttcctgc
      781 agcgcatagg ccaatgaggg gatgcggtga ttaagtgccg tagcctgcac ggtgaacatc
      841 ggatcacgga atacggtaaa gggcccctgg cccgtgcaac cgttcagcgg tgcatccggt
      901 tcatcgtggc gttgaaagcc gtccgttgcg aggaaggtcg ccgtctggat gccgtcggga
      961 tggaactcgt gcgcagtaat cgtgagcggg tatccgtcca ccaggttcca tgtgtagccg
     1021 tgaagttttc cttgaacatt tgggatgagg cccggtggcc cgtgacgccg cagagttttg
     1081 ccgcgcccga gggcgacgcg cagcagagca tcaaacccga tgaagtgatc catatgggta
     1141 tgggaaatga agatgtcgct ggcccgcagc agccgtgtcg ggcctagggc gtcgttgtga
     1201 ccgaggtcaa ataggagggc gcgtttggac cagcgcactt cgacgaacag cccggggtcg
     1261 ccgaatacat cattcacgag atagctggag aaggacgggt tcatgggtgc cgcatcacag
     1321 agccacgaat agtgagtccg tcgtgagtgc aactgttgct tgaagttccg ttaccggaac
     1381 ttcaagcaag gtcaggtcaa tatggttcgc atgacgctat agaggacgat aacctccaca
     1441 gcatgggcga cgatcgtgac atacagattt ctgaaggcga cccacagact tccccagatc
     1501 aggccatccc agacggcaat ccaatggaga tgatgaaagg ttgccaccag aaagggatcg
     1561 aacgcaaaca gcagggcgct caatggtacg gcgagggcgg agggcaatcc cagacccagc
     1621 agccggccta acagaaaacc gcgaaagttc agttcgacca tggcggcgat catgaggatg
     1681 aaccagggca gcatgaggag cagcggaatt ttggcgtgcg gcgtgtcggc gagaaatcga
     1741 tagtctgccc caagccaggg cacgagatag aggatcacca ggacgttggc tgtgccgagg
     1801 gcgagccccg ttgcgatgcc caagcggagt ccccttggga tgagggcgcg cgtgagcccg
     1861 agcctcgtaa ccgttgcgcc gttcaggcgg ctccaggtca acagcgcggc ataggcgcaa
     1921 agctgaggga ggaactgata caggggctcc tgccgaagtt cacgagggga cgcgtaatag
     1981 aggagtgtag caatgagggg aagcagcgcg agggccgccc cccgttcatt gtgctgaatc
     2041 ggatacgcgg ccgacgcctc ctcaggcctg atcatgagca gttattcaag tcgcagacta
     2101 taccgttcga gtgtggtggc cttattcctg gccaggttcg acaaggattt gttgtagtcc
     2161 accgtggctc gcagctcgtt gccttgcgcc gtggccaggt cacgttgaaa gtcgagcacg
     2221 aatcgcgtcg tactgagacc gaccttcaag cgttcttgtt cggcctgcaa ctgcttttcg
     2281 gccatgatac gagccgagcg ggtggtttcg attcgtttga aatcggtgtg tacgcggcgg
     2341 acggcttccc ggacaccgac gatcacttgt tgtcgaacgc tctgtaagga ggcctgggca
     2401 ttccgggact ccaattgccg tttattgtag gtgctatagg ccgagcggtt gccgatcggg
     2461 tagctgagga ctaatcccgc cccgtaatta taaaagtctc cgccgaaatt ccgtctcgtg
     2521 gcgtccccgt agtcggctcc aagtccggac agtcccatcg tcccttgcac cgacagggtg
     2581 gggagcaatt gattcttggc aaacttcaca ttgaggtcgc tgctctccac gttcttgccg
     2641 gcctgcaaaa cttccgggcg gcgttccatg gcgatatcga tagcttcctg aagactgatc
     2701 gcttccagcg aggtggtcgg tggatcggtc ggcataaggc gcagatcctg ccgcagttct
     2761 tcctccgctg gatttaagag gcggcgcagc tggtcttcct gatcgcgaat ggatttttcg
     2821 gccacgagga tctgttcgac acgcgaagcg acggccgcct ccgcctgcag gacgtcgacg
     2881 atcgacatga cgccggcctt ggctttggcg cggttgctgg ccaggagttc ttccgctgcc
     2941 ttgagtgccg cttgcgcgac tttcagattt tcattcgcaa agaccatctc ccagaatgtc
     3001 tgctcgacgg tagcaatgac agtgagcacg cgatcgagaa acacatgttg ttcgacggtg
     3061 gcattgtttt gtgcgatcga aatgaacgtc cggttgacat ccgtgccgaa gtttctcagc
     3121 agcggctgtg taaccgttaa cgcgagcccc ccggtccagg ccgggttgaa caggaaggtg
     3181 ttgggcccgc tgacatagct gcgttggggg ctgtaattca gatcatagtt tgcgccggtc
     3241 gggagattct gggtgatgtc ggcggtgacg ctggaggtgt tctggtcgaa tttggtgatg
     3301 tcctgcaggt tggcgccggt aaatccgagg atgggtcgat tcagcggcgc gacctgtctg
     3361 ttgtattgac cgttcaagct gatggtcgga tcgaattttg cctgttcaat aataatgtcg
     3421 gccaagcggc tttctttggt ttgtcggccg atatggatat cgaggttatt ctgcagtgct
     3481 ttgaggacag cgtcggccaa cgacatggct tcgcgtcgtt cggtcgggac gggtttggtc
     3541 acttccagac cgaatgaggt ggacgccgtc cagagcaaca gcgccgtcgc gccgccgatg
     3601 agcagacagc cccggagcat tggattcgtc ataacaaact ccttgatggg agatgcaggt
     3661 tgagccatac tgaattcatc atgatggtgg cgtggtatca caaacggcga tcagtgtcca
     3721 tgctggcgcg caagggcagg gcgaccatgc gctcgaccgg gatcatgctg gttgctgtaa
     3781 cagccgcact gctctgggtt tcgccggcgg ccgcgcaatc ggtatcaggt gtgcgcggat
     3841 ttaatggagg tgtggcaccg ctctttggtc tcgtgggtcc agggaatctc tacattgata
     3901 accaggggac gcagggattc atctacaatt tcggcaataa cttcgaatcg tacaatttcc
     3961 ggaatcccac gaccggtcag gcatggagcg gcgccgtcat gacgctgggg ccgcaattgt
     4021 cggtgggact cattcaaggc gccaatcagt cgggttctcc cgtggtgttt cctccagtgc
     4081 cgcgtgagac cgcacctctg cctccgattc aatccggtct gctcgaggat attccctagc
     4141 cagctgctgt tctcgtcaag aacttggcct gcgttcagca ggtgcacgta cactccgcgt
     4201 acgtttcctc cccatcgttt ttcttcttcg gatgtccttg cctctgttca gccacacaat
     4261 cttttcagag tcggaaggtc cgtctttgtg gcggtttggt gtgtggctgc aagtctgtca
     4321 cagcggagag ccgtagggct cttcattatc cacagaatcc acagcctgaa cgtcacctgt
     4381 cgatcgttgc aaagtggcgg gaggatattc gtaaggcgtt gatttataac gtacgtgttg
     4441 attgtatgca ttgccaattg atgaggaagt catcgacggt atggcgcttg ctttgactcc
     4501 gcgtcattgc gatcgatctc ctgtttcgta acgagacgac aaaaacaatg cgctcctgtc
     4561 aaagatgtca attgaatcgc atggggagaa cggaaagaat gccgacagac ggtgggaggc
     4621 gcggtgaagt gccagccgcc ggttttcagc ccccgtcacc gagcatctac gatcaaagga
     4681 gattgtttgt atgaggattg cccaggtatc gccgctttgg gagagcgtcc ctccgaaatt
     4741 gtatggaggc actgagcgca tcgtctccta cttgacggaa gaactcgtcc gtcaaggtca
     4801 tgaggtgacg ttgtttgcca gcggtgattc ggtgaccaag gcgaagttgg aatccccgtg
     4861 tcagcaggcg ttgcgtctca acaccggcat tttcaaccgc gaagcaccgc tcattcagat
     4921 gatggagcag gtgttcgctg ccgccgacca gttcgatctc atccactccc atctcgactt
     4981 tttggccttt tccctttccc gccgctgtcg cgtgccggtg gtgaccacgc ttcacgggcg
     5041 gttggacttg cccgagcttg tgccggtctt ccgtgatttt gcggagctgc cgctggtttc
     5101 catttcggac tcgcagcgcc ggcccttgcc ctggtgtaac tgggccaaca ccgtctatca
     5161 cgggttgccg aaagatctgt ataagttcaa cgccgaaccg ggcaagtact tggccttcct
     5221 cggccgcgtc tctccggaaa aatgtccgga tcaagccatt gagttggcca aacgtgtcgg
     5281 cttgcccatg aagatggccg cgaaggtcga tcccgcagac cgtgcctact ttgagcgagt
     5341 ggtggagccg ttgctcgacc atccgttgat cgagttcgtc ggagaaatca ccgatgcaga
     5401 aaagagcgac tttatcggga acgccatcgg gctcgtctgt ccctatgact ggcctgaacc
     5461 gttcggcctc gtattgatcg aaagcctcgc ctgtggtaca cccgtcctcg cctaccgccg
     5521 gggatctatt ccagaaatca tcggtcatgg tgtgacgggc ttcatcagtg aaaacctcga
     5581 tgaaatggtg agccaggtgt cgaagctggg cacgatcgat cgccatcgat gtcgccaggt
     5641 ctttgatgaa cggttcaccg cccagcgcat gaccaacgat tacgtcaaga tctatcaaca
     5701 actcattgcg gatgccgcta cgcttccggg taaagccggg cagcagcacg cttcgaacct
     5761 ctagtcagcg cgatctcaac ctcgcggaag gaccagtatg gcagacgagt cgcagtccac
     5821 tcctgaatcc aatgtcaccg gatggcggtt gttggcgacc cgagactttg gatgtctctg
     5881 ggccggccaa gtcatctccc aaatcggtga cggactgaac aaggttgcgc ttctgtggtt
     5941 cgtgtatgaa atgaccgggt cggcgctcaa gatgaccgcc atcgggttat tgcagacgat
     6001 tccgcctctg gtcttcggtc ccctcatcgg cgtctatctc gactatctcc cgaaaaaaac
     6061 ggtcatgatc gtcgtcgatc tgttacgaac gttgatggtg ctgttgatac cgctcttcta
     6121 cgcgctggat ctgttgacgc ttgagcgcct ctatgtgctc gtcttcctgg tctccattgt
     6181 gtccactata ttcggaccgg cgttggcctc ggctgtgcct ctgattgtcc agcggtcgca
     6241 actgacgacg gcgaatgcgt ttctgcagag cactacgaac atcggcgtct tgttgggacc
     6301 ggccatgagc ggtctgggta tcgctttgat cggcgcgcaa aatgtgctgt atgtggatgc
     6361 tgccaccttc ctgctctccg ccctcttctt attcccgatt cgtgtgagag agaccaaatc
     6421 ggtgaaggat ctggatgtcc tggggactcc cgtcatgcag gacatgatgg tgggattccg
     6481 cttcgtcttc ctgcagcacc gagtggtgtt tgcattgatg attacggccg tcctctataa
     6541 tctggcgatc agcgcctttg tgtttctcct ccctgtggtg gcaaaggaac tgttgcaagt
     6601 cggaccgatg gagttgggat ggttgtggtc tgccctaggc atcgggatgc tggcggcatc
     6661 aatttggttg gctcggatgc cccagggcac attccaggag cgtatcggca agatcggtcg
     6721 ctcgttgacg atcgggggcc tcgcggtgtg cgcgttggga ttgattcaaa ctccggtgtt
     6781 gttcagcacg tttttattga tcatcatcat cggtggtagc acctccctgt tctacccggt
     6841 cgtgtgggcc atgctccagg aagtgacgcc cgaacatctg ctcgggcggg tctttaccac
     6901 gtttagtgtc ggtgggatgg cgtcggccat ggtgggcatg gcgggattcg gctgggccgc
     6961 cgacaccctt gggcctgccg tcagtctcat cggtatcgga ctgctcctct tgctcactgc
     7021 catggtcacg atgcaggtta gccgtcgtgg cctggtggtg actccggctg tcgcataacc
     7081 tcccttccag gacgatcgtt tcggtctatg cggcagctcg ctggtatgcg ccacgcgagc
     7141 tcaccccgct tgaccaatgc cggcgatggg cggagttgct ttctgcccct ccgtcgcgta
     7201 tgtcttttct ctcaggctgt tcgaagacga atccgggcct gccttctcgg ctcggtttac
     7261 ttttacacat gaggggcata tgcctatgcg gactattcag accgtgtgtg tggcagggat
     7321 tttgtcgttt gtggtggcgt ccgggcttgg gagcgtgagg tcgatggcct cggagccgca
     7381 gccgtcagag acaaaacccg acgttgcacc tggttcggga tcgaaggatg ccgaaggaaa
     7441 gataaaggaa ccggatacga aaccaaaaga gccggagtcc aaaccaaaag agccggaggc
     7501 caagccgact gagcaaaaat caaaggacag cgatacaaag gcgaaagagc ctgaaccgaa
     7561 ggccaaggac ggggaagcaa aacccaaagg tcaagaaccc aaagccaagg aatcgacgac
     7621 caagcccaag gaatcggaac ataaggcgaa ggagggtgag tccaaatcct cgaaagagcc
     7681 ggaccaaaaa tccaaggagc cggaagcgaa accgaaggat gccgagattg ccgctgagca
     7741 tccctgtccc tcggtgccgc aagcggctga cgcgaagccg caggtggaag cgccggtccc
     7801 ttctggtgat tccggtacgg gagagcgtca gaaatcggaa ccggaaccca agaagccggt
     7861 ccgctcatca atggtgacgg ccaagttggc cctgatggcc gacccccatc tgtttcccta
     7921 cgatatcgaa gtggatgcca aggataaaga ccttgtcttg ttgggaaaag ttgggcagga
     7981 gtcggataag cgtgtggcca ccgatatcat acgatgcctc gaaggcgtgc atgccgtaga
     8041 aaaccggctc aaagtcgagg cggacgccgc gcatgggttg ttcgcggagc gtgacaaaat
     8101 cattacccaa ttggtgaagg agcggttcga gaaaagcaaa accttgcaat cggtcaagtt
     8161 cgatgtgaaa acagaggacg gcgtcgtcac cctgaacgga gcgacgcggt ttcagatcat
     8221 cgtcctcgaa gcggcacagg cggcacggca ggtaccgggt gtccgagcgg tgaataccga
     8281 tgcggtccgc ttggtcgccg gagagtaagc gcggttcatg aggccgatgc cacaggaggt
     8341 tcgacacgca gtctgctgac gctatagaac cgggacgcgc aagcggcacc gggccaggac
     8401 gccccaacgt caagtggtcg ccgcatctgt tgacgcgcga tttcacgctg gtctggtggg
     8461 gccagatggt ctcgcagatc ggcgatggcg tgtcgaaact cgcgctgctc tggttcgtct
     8521 attccattac cggctctcct ctgaaaacca ccatgatcgg gctgttgcag acgctaccgc
     8581 ccattctgtt cggtcccttc atcggtgtga tcgtcgatcg tgtccccaaa aaactcctgc
     8641 tgatcagtag cgacttgatt cgagcgttag tgctgggtgt gttgccctgc ttgctgccgg
     8701 tagactcgtt cagcattgag cggctgtatc tcatggtgtt cgttcatgcg gtggcgtctg
     8761 ccgtgttcgg tcctgccctg acggccgcga ttccttccct ggtgtcccgc catgaattca
     8821 cggcggccaa cgccttgctg cagaccacga ccagcatcgg catcatcgtc ggcccggcgc
     8881 tgagcggcgt cggcattgcg acgatgagtt ctcaagaagt gctctgcgtg aatgccgtca
     8941 gttatgtgat atcagcgctg tgctttttgt tgatccgctt tccgcgcacg gaaacggtgc
     9001 cggctgcgga ggggggctcc ctggccggaa ccctgcggga cgtgatggaa gggtttcagt
     9061 atgtgctcca tcggcagcgg gtcatcctca tgctcatcgg cgcagcctcc atgtatacct
     9121 tcgccactag tgcgttcagc acgttgtttc cggtgttcgg caaaaagctg cttgatctgg
     9181 ggcccatcga agtcgggtat ttgtggtcgg ccttcggtgt gggattgctc ttggtctcgc
     9241 tgggacttgt gtcgctgtcg tcctggtcgc tgccgaagcg gattcaattg atggcggtgt
     9301 ccagtttcat cagcggccta acccttctgg gtttgctctt cacatcgaac cggttggtag
     9361 ccgccgtgct catggtcatc atcggcatgg gcgcgggcac gctgaccccg gtggcctggg
     9421 gtgtcttgca ggaaattgcg cccacgacgt tattggggcg agtgctggct atctacaatc
     9481 ttggggcgat gacctctgcg atcgccggca tgacgatttt cggctgggtg acgcaggaat
     9541 tcggtgagcg tcccagcgta tttggaatcg gggtgggtct gtttttatcg gcggtggtgt
     9601 cctggcgagt ggcccgatgg gtgcaggtca attggtccga gacgagcgtg cctgccgaag
     9661 aagccgcccg gtccgttgtt atggtgacgc agcccacaag tcattgagtc cacatccgta
     9721 tcatatgatt gcacgggtcg agaggccctg tccaaagacg aagcgagcag caggcaggaa
     9781 ttggtgaggt gtgatggcac aggccgcgct cattgcatcg atcgttcagg gatgacggga
     9841 ggaaaacgtg gaagaaatca ttagcgtgaa tgatcagttc tatatcctgg ccagttcatc
     9901 gatggccgat gaccggacgc gtgtgctcaa gcatggagaa accttcggtg tgtttgaccg
     9961 gtatggcgac attcaaccgg taggccgggg cacacaaggc gtgttccatc aagggactcg
    10021 gtttctctcg cggcaggaaa tatttctgaa caatgatcgg ccgatgctgt tgagctcgac
    10081 ggtaaaggaa gacaacgcct tgctggccgt ggatctgacc aacccggact tgtatcggga
    10141 cggacgcatc gccatcccgc gcggcagcgt acacgtcttt cgctctcgtt ttctctggaa
    10201 tggcgtcagt tatgagcggt ttcgtttgtc gaactacagc ctggcgccag tgaagatgac
    10261 cctctcgatc cgattcgagg ccgacttcgc cgacatcttt gaggtgcgcg gtaaaaaacg
    10321 cgagcgcaaa ggccgcatgt tgccgaacat cttacggaaa gatctcctgg tgctgcgata
    10381 cgaaggtctc gacgaagtga cgcgggaggc ccggattcag tttgtcccgg agccgttgga
    10441 gattaccgcg tcacaagcca ccttcggcat cgaactggaa ccgaaagggg agatagccgt
    10501 agcggtcatg gtggcctgcg atgaggtgga cagtcatcgg ccgctcttgt cttatgatgc
    10561 ggcgatggca gaagccggtt tggcattcgg cgccgagcag acggaagact gtaccatcca
    10621 aacgtccaac gcgcagttca atgagtggtg gaaccgttcc gtgttggacg ttcgcatgat
    10681 ggtcaccgat acgagtgaag gtccgtatcc ctacgccggt gtgccctggt tcagcacccc
    10741 cttcgggcgg gatggcgtga tcacggcgat ggagtgtttg tggatcagac cggaattggc
    10801 gcgtggggta ttggcttatc tggcgtccac tcaagcgaag gaggtcaatc ctgctcagga
    10861 tgccgagccg gggaagattc ttcacgaaac ccgcaagggc gaaatggcgg cgctgcacga
    10921 aattcccttc gggctctatt atggcagcgt cgattccacc ccgttattcg tgatgctggc
    10981 gggggcctat tacgagcgca cggcagacct gccctttatt caatccatct ggcccaatct
    11041 ggaagcggca ttgacctgga tggatacgta tggggatatc gatcgcgatg gtttcgtcga
    11101 gtatgtgcgg aaatcgccca ctggcttgga taaccaggga tggaaagact cccatgattc
    11161 catctcgcac gcggacggtt ccttggccga aggaccgatc gcgttgtgcg aagtgcaggg
    11221 ctatgtgtat gacgccaagg ttcaggcgtc gaaactggcg gaagccttgg gctacgtcga
    11281 tcgtgccagt cagctgcgac ggcaggctcg gtcactcaag gaacggttcg aggaggcctt
    11341 ctggtgcgaa gcctcgtcga cctatgctct tgccctggat gggcacaagc gcccctgcga
    11401 agtgaagacg tcgaacgccg gtcactgtct ctacacaggc attgccagcg atgagcatgc
    11461 gcggcgcgtg gcggagacgc tcatgtctga tgagttattc agcgggtggg gcgtgcggac
    11521 cctggccgat tccgaacgtc ggtataaccc gatgtcctac cacaacggat cgatctggcc
    11581 acatgacaat gcgatgatcg ctgtcggtct cgcgcggtat ggcctcaagg ccggcgtgga
    11641 aaaaatcatg accgggatgt tcgaggtgag tctcgtcctc gattttcacc ggttgccgga
    11701 actgttctgc gggtttgtcc gtcgtcccgg ccaaggcctc acccgatatc ctgtcgcctg
    11761 taatccgcag gcctgggcgg ccggatccgc ctttatggtc ctccaggcct gcctgggctt
    11821 gtcgattgtc gcgtcggaac agaaagtgat cttcaaccat ccgattcttc cggactttat
    11881 cgacaagatg cagatcaaaa atctgacggt cggcaccgcg tcggtggacc tgttgcttcg
    11941 gcggcatgac ctcgatgtcg ggatcacggt catccggcgg gtcggtaaag tcgaggtggt
    12001 ttcggtgaag tagcgcgggc aatacgtggc ccgctccgct gcgttatggc gcgtctgcca
    12061 gctgccacag catggcgagg acatatcggg cagcctctga aagaatatca gggttgatag
    12121 tctcggcggt gtctgtgggc tggtggaaat ggggatgaat tccgccgctc accaccgtga
    12181 ccgtgggaat gcccgcctcc ttgaacggca catgatctcc gccggggaaa aagccgaaga
    12241 catcaaggcg gtctgctacg cctgccattt gccccgcctg ctgcgcggca gccttatcca
    12301 tcccggtgac accgatcgtc aggcgtccgt tgccgaccgc tgcgtggtcg atattgatca
    12361 tccccaaggt cgaacgtaag ggcgcagtcg gctggctgat atagaatttg gatcccacca
    12421 acccctgttc ttctccgctg aacgaaatca ggacaaccga ccgtttcggc tggaccgggg
    12481 cggcttgcag cacgcgcgcg atctccagca gcacggctgt tccggaggcg ttatcgtcgg
    12541 ctcctgggaa cagcaggccg ccttggcgtc caaaatgatc gcggtgcgca ccgatcacga
    12601 tcgtttcttc tgccttcgcc tggtcgcttc ccggtacggt caagacgacg ttgtgaagca
    12661 ggccgtcctg gactgcgctg tgccaacgca tggtaacgac ggtatccgtc gcgatcgatt
    12721 gcggtggctg gccttcattg aggtgttgct gcaacgtccg tagccgatcc tcgtggcctg
    12781 ttcggtcagt gtggagtatg gccatcgcgc gggtggtgct gatccaggca ccgggaatcg
    12841 tacgatccgg ctccgttaag ccgtaaaagg cgctcggtcg accggtgact ccgcggcgag
    12901 tttcataagc attgaggatt ggtccggtgg cagtgaggta ggccagcgcc ccatgggcgt
    12961 gagcggcatg ggccttatcc gcatgtgaca cctgcttggc gtatcgctcg ggtttgccac
    13021 gcaaaaagag cacgaccttc tgacgaacat cgaggcccgc gtattcgtcg aagccacttt
    13081 ccgggtcgga gattccgtat ccaacaaaca cgaccggcgc ctgcacgtcg gccgaagggg
    13141 agtcgaggac gggaaggtaa tcggggccga tctggtccgg cggcgaatcg gcactgcccg
    13201 ccaggcgcag gaacgaatcc ttgccgatga tcgttggccg atgggaagtg gactgtatgc
    13261 gaatcgatgc atgggcggga gaacccttgc tgcgagattc ctgcaaggga ggctgtgagc
    13321 tgttcgattc caggaagcgt tgccgtacga attcagcgga ggccaggtcg tcggaggtgc
    13381 cggtctggcg gccattgaag gttggtccac tcagtgttcg gacatcctcc accatgcgtt
    13441 ggctggaaac gagctccaag gattgtctga gcgactgagg aacttccagc tggttctcgg
    13501 ccagcaccgt cacaggcgtc agcgcaatcc agaggctgag gatgagttga cccactaaat
    13561 tggagtgatg gctttgggcg gacaatgagg ctctcctgct gtgaggacct acgtcagcga
    13621 atggcgtcag atcgaagtgg cgtgtgcttt ctgggccgac caacgttgat accagcgcca
    13681 gaggggtcgt tgcagaaact gtggaagcgg atatcccagt cctcgagtat agccgtcatc
    13741 tcgatcgagt acggcgaacg cccactcata tttcttttga aaggctgcgc agcgctcctg
    13801 gatttcttcg gtgctgagtg gaatgtgttg ccgaagggcc gccccgatcg cctcatcgga
    13861 ggtcatgtca cggacaagcg actcgatctg ggcgggcgtg agccggaggt ggcgcatgaa
    13921 ggactggtcc aatgctgaag ggtaggcata ggcgccgagc gtgccggcct gaaatgcccg
    13981 caccttgtcg atcaggcggg gtaaccagat caatccggcc gtggcgtcgc tccatcggcg
    14041 gggtggatgc tgtcgaagat ccatgcgtga tccaggtttc agctcaatga ggcggccttc
    14101 ggaatgaggc actatagcat aagggaagag ccccgttgca gagagcaaag ggctcaaggt
    14161 actatcggtt cccgggccgg caaaggatca gtattccgct gcatgaggcg gtcccggatc
    14221 gcaacagcag ctcgactgat aacgcaaagg gaatcagcta tggcagagca gggaaaaggt
    14281 ctctacgact ctcagtacgc acggtttcga gacaatcagg ggacacatca gggctatgaa
    14341 tttcagcacg ggccggcagg cagtgtgcct ccgcccgtgg ttacgttcat ggataaaata
    14401 aaatggtgga cgttacgcgg atggcgacgg aagaagatct tggctgaacg tgatcgatat
    14461 cagcaggaca tcatccagat caaaacagca catccgaaat cgtgaggtcc actcgaggag
    14521 gagtacttcc gtgcactatg gatatcagcg cgtcaggcag atacaggtag gtcggaaaaa
    14581 tcagtggggt ggtgtcacat cgtttgtcag ggccctgcga tatgttggcg tggcggccgt
    14641 cgtgggtgtg ttggcgggtg ggatggagac ggcatgggct gtgccgaagg aaacctccac
    14701 ggaaaaagcg gagcatttgc ggcaacaggc caatccccaa ctcttcaacg attggacgtt
    14761 tgataaagat gaagtcgggg ccattcccaa ggggttcgtg gccgtgacct ccggcgatca
    14821 acggccaggt tcgtggaagg tcgaggcgca agcagccgtg ccgtctgctc ccaatgtgtt
    14881 gctcgggaca tccggctgcg gagagtgtca ggaaattctt gttgcgcagg gatttcagta
    14941 tgagtacccc gatctggtcg ttcgcattca acaggggggc ggggctgcca ccgggcaggt
    15001 gggtgtcgtg tttgggatga aggatgccca gaattactac gcgactgtcg tggatgtgtc
    15061 tcagaaaatt attcaggttg tgcgcgtgat cgaggggaaa gagtcagtcc tcggccgggc
    15121 ggcaatcaag ccgaagccgg ttgaatggca cacccttcgc gtacagcgga ataccattat
    15181 cagcaaggat ttcgtcgaaa cattctttga cggaagtctg gccttgtccg tggaagatca
    15241 ggcgcttgga gtggggcagg tgggggtatt ggtgaggggg gagtcgtcgg cacgcttcga
    15301 taatttcaat gcggccccct tatattccag ccgaccgttg tctgctccgg cggcctattg
    15361 agccagcaaa agtgacattc ggccggatcg gatagatttt tggcgagacg atttcgcgct
    15421 tgcctttccc attttggtgc gatacagtga aggtgcttca ttgcgaagag ccttggcgat
    15481 gaagtagaaa aagaattaca gggctcgtgt cgatgttgtt ttgggttctt cccgggaatt
    15541 tcggaagcca gagctaagat tgccgctgtg tttgcatctt cgccccattc cttcctcgtt
    15601 tctttctcga gtcccagaca acaccattct attctcaaag gaggaaccca atgggttcga
    15661 agatctacgt tggcggcttg ccatattcaa ccaccgaaca acagctgagt gacctgttcg
    15721 cggtgcatgg cgcggtgacg tctgcgcgca tcatcacgga caagttcacc ggccaatcac
    15781 ggggctttgg tttcgtggaa atgtcgggag attctgaggc acaggcggcc atcaatgcat
    15841 tgaacgggac acaattcggt ggccgcacct tgacggtcaa cgaagcccgc ccgcaggagc
    15901 cccgctccgg tggcggtgga cgtggaatgg gcgggggccg gcactaagct gcgcccgttt
    15961 ctatatacct attcaggggc tgccgacagg gtagcccctg cgtttttctt accgtgttgt
    16021 cctctctttg gtctgcaggg ccatcaggtt gtctgcccct gtttccccag atagagattt
    16081 aggagtgtcg ttgcatggtg tcgtttgccg aattgtctct tacatcgttt ctcgctgacc
    16141 gtttacagca ggccgggttt accgcgccga ctcccattca gagcgctgcg attcctctgg
    16201 cgctgcaggg gcgtgatctg ttagcccagg ccaaaacggg gagcgggaaa acgttggcgt
    16261 ttctcattcc gttgattgaa cgtgccgtca aggaagggtg gaagcctgcc ggccatgcgt
    16321 ccgcgcaaac cgggtcgtca cggtcgccgc gtgccctggt gttggcgccg acccgtgagt
    16381 tggccctcca gattgaaatg gaactgcgca agtatgcgcc accggcggtg acgtcgctgg
    16441 ctgtgtacgg cggtgtgccc atcgagcggc actaccgtgc cctccgtcag ccgccgatga
    16501 tcgtcatcgg gacgccgggg cgtctgctgg atgtggccgg cactcggcat ttggatttgc
    16561 gcggcatcga atatgtcgtg atggatgaag ccgatcaaat gctcgatcgg gggttcctgc
    16621 gcgacatcca acgtatttta cagctgcttc cctctcagcg gcagaccatg ttgttttcgg
    16681 cgacgttctc gccggagatt cttacgctgg cagaatcgat gctgaagaat cctgctcgga
    16741 cggcggtgga tcccggcgtc aacacgccaa ccaccatcac gcatgcctat tacgtggtgc
    16801 ccagcgaagc atcgcgggtg cagctgattc atacgctgct gcaatcatcg gaagccggtg
    16861 atcagtcgat ggtgttttgt gaccagaagt acaaagtgaa gcgcctggct gcccggttgg
    16921 gcggagaacc ggcttcggtg ggggctatta ccggcaatca ctcgcaggcg cagcgtgagc
    16981 ggacgctgac ggcgttccgt tctggtcggt tgcggtcgtt ggtggccacc gatgttgcgg
    17041 cgcgcggatt ggatgttccg accgtttcgc aggtcattca ttatgagttg cctggcaacc
    17101 cgacttcgta cgttcaccgt accggccgga cgggccgagc ggagcgatcc ggcgcaaccc
    17161 tcttgattct ctctccgcaa gaggagcatg aatatttggc catggtgcga cggttgcgaa
    17221 tccaaacgaa gcgcctgaca ctgccgacct tggcaacgtt gccgcctccc gcgcgtgaga
    17281 cggagtcgaa cagccatccc cggcatgatg gacacgggcg tgatgcccgg ccgcgtcgtt
    17341 ccggagatcg ccctaactcc acgagccagg acgccagagg gggacaggga cgtcgcggat
    17401 ggcgtgccgg tcgtcctgcc gctccgcgcc gcggcaatag ttgatgcagt atttggccgg
    17461 atggaaatgc tgtccggcag attgaggagg atgccgcgat gagttctgca cggaatctct
    17521 tgctgagcat gatggtcgcc gccggttgtc ttgcagccgg cgagggcgcc ttcgcgcaaa
    17581 gcgatcttgc cattgccgac ggcatgaagg tgtcgttgga gtatacgttg tcgcttcccg
    17641 acaaaagcgt ggccgattcg aatgtcgggc aggagccgat aacgtttgtg cagggggcgc
    17701 atgaaatcgt tcccggcctc gaaaaggcgc ttgacggcat gaaggccgga cagaagcggc
    17761 gtatcgatgt gtcggcacag gacgcctacg gcccctacaa caataagctg cggcaaagta
    17821 tcgataagga taagcttccc aaggacgtga aggtcgggga cattttgcag gcttcggaca
    17881 accggctggt caaagtcttg gaagtgaatg acaagaaagt tctgatcgat ctcaatcatc
    17941 cactggcagg aaagacactc acgtttgacg tgaatatcct caaggtggaa aaaggcgatg
    18001 ccgccgacgt tcctgagaag aaaaatccct gaccgatcgc aatgcaccta agcggtgagc
    18061 aggcccgctg atcgggctgg ttgtaggttc tcgcggtaag atgccctccg actcgccgtg
    18121 gcgatcagcg tcacggcggc acagtgtgcg ttcgagtctc ccttcaacga tgtaaacgcg
    18181 atgtcatttc agaggtggcg tgtcttcgag acatgccccg aagatcatgg cgtcctgctg
    18241 ctcggtcaca tcgacagtcg gcaaacttca tcgggttttc aggccgggcg atttggttgg
    18301 cgcgcatgtt aagatgacgt gattatgccc ccgtttaagc tcgatgctcc attcaaaccc
    18361 tgcggcgacc aaggccaagc gattgacaag ctgacgtccg gaattttggc cggcaagcag
    18421 catcaagcgt tgctcggcgt caccggttcc ggcaagacct tcacgatggc gaatgtcgtc
    18481 gagcgggtgc agaagccgac tctcgtgctc gttcacaata agacgttggc cgggcagctc
    18541 taccaggaat tcaaacagtt ttttccgcac aacgccgtcg agtacttcat cagttactac
    18601 gattactacc agccggaagc gtacattccg cagagcgaca cgtatattgc gaaggatgcc
    18661 tcaatcaacg atgctatcga tcagatgcgc catgccgcca cgacatcgct cctgcagcgg
    18721 aatgatgtct tgatcgtctc gtccgtttcc tgcatctacg gcctcgggtc gccggaggtc
    18781 taccatgaca tgttggtcta tctcgaagag gggatggaga cgcgccgcga gaagattctg
    18841 gccaaactgg tcgatattca atatgcacgc aatgacgtgg atttccaccg tggaacattt
    18901 cgcgcgcgcg gcgatgtcat cgagattttc ccggcttcgt cggaagccaa gtcggtgcgc
    18961 atcgagctgt tcggcgatac agtcgatgcc atccatgaga tcgatccgct gaccggaaaa
    19021 tcgctgggca agctgccgaa gatcgccatc taccccaata cgcactacct cattgcgccc
    19081 gatcgttacg aacgggcgat taccggtatc gaggaggagc ttgaggcgcg agtggccgcg
    19141 ttcaggaaga acggtcagtt attggaggcg caacggatcg agcagcgaac caaattcgat
    19201 ctcgaaatga ttcgtgccat ggggtattgc cacgggatcg aaaactattc gcgccatttg
    19261 agcgggcgtg caccgggaga tccgccgccg acgttgctcg attatttccc caaagacttt
    19321 ctcttgatca tcgatgaatc gcacgcgacg gtaccgcaga tcggcggcat gtacgaaggt
    19381 gactattcca ggaaacgcac gcttgtggac tatggcttcc ggctgccgtc tgctgtcgac
    19441 aatcgtccgc tgaagtttgc tgagttcgaa cagatgctca aacaggtcat ctatgtctcg
    19501 gcgacgccgg gcccctatga actggagcat gccaagggtg aagttatcga gcagatcatc
    19561 cgcccgaccg gcttaatgga tcctctgatc gacgtgcgat cggcgaaggg gcaggtggac
    19621 aatctgttgg cggaggtccg ggcggaagca gcgaagggca atcgcgttct cgtgacaacc
    19681 ttgaccaagc gcatggccga agacctcacc gagtattatc atgacctcgg ggtgaaagtg
    19741 cggtatctgc actcggacat taaaacgctg gagcgggcgg aaattattcg cgagctccga
    19801 tgcggcgtgt tcgatgtgct ggtcgggatt aacctgttgc gagagggact ggaccttccg
    19861 gaggtcggat tagtggcgat tctggatgcg gataaggaag gatttctccg gtctcatcgg
    19921 tctctgattc aaacagccgg ccgcgcggcg agaaatctcg atggccgagt cattttttat
    19981 ggggatacgg tgactgactc gatgcggagc gcgatggatg agacggcgcg ccggcgccat
    20041 atccaggagg catacaatgt cgcgaatggc attacgccgg aaagcattaa gaaactgatc
    20101 ccggtgctcg actatgcgtc gggaggaacg aatgccggtc agcttgagct tgcggcggaa
    20161 tcgttggcgg actatgcgcc cacagcggat acggagcaat tgatccggcg tctggaggtc
    20221 gagatgaaag ccgccgccaa gaagttggag tttgaacggg cggcagagtt gcggaaccga
    20281 atcaggtcct tgaagctgaa agcgctggag ttcaccgagt aacgatcgag ctgcgagacg
    20341 gcgacggttg ggaatggcct caacttacat ctgcttgtag agataggctc ccaggaacat
    20401 tcctaacacg ctaagcagat tgaccttaat gctgaaacca aaggtcagtt tgaggagaac
    20461 cagatccacc gtgaggggtg ggttgattcc gggagagaaa ttgctggcaa agatgttttg
    20521 aatcgcgccg ttcggggcca tcacgcgcag aatttcacca aaaatcccgc ccagcattcc
    20581 gccgatcagg ataaagatca gcagcacgcc gatcgatttt ctcatccgat gtctctcccg
    20641 gttcacactg tcggcggtat gtgccgactt gaggcaccat atcggaagcg taaaatgatg
    20701 tcaacaaaat gcatgccaga tgctagcagt ggcgcatgtt cctggcggga aaggccctgc
    20761 cgtgctccac ggtggaattc tttcttcgcc gttcggtccc gcgccctata tcacgaccgt
    20821 ttttggctgg ccgagggctg gcggtgatgt cgagacaggt gacgtggcga gtcttccgga
    20881 cgtcgccaat agagtgccga atatcttgac ttcacgcgcc gcccttctat agactgcccg
    20941 atgctatttt tttgatgttt ttcgaggcga tcggagctct ggctcgaacc cggcttcgat
    21001 ggcctttttg tttggtgatt ggtgcttgac gcgctaagcg aaaaatttga acggatcctc
    21061 aagaagcttc gtgggcaagg tgtgctcacg gaggagaata ttgccgaagc gctgaaggaa
    21121 gtccgtctcg cgttgcttga agcggacgtg aatttcaaag tcgtgaagga ttttctggat
    21181 cgcgttcggg aaaaagcgat cggtcaggaa gtgctgaaaa gcctgacgcc tggtcatcag
    21241 gtcgtcaaag tcgtgtggga cgaactccgc ggcatgatgg gcggagaacg gtccggtatc
    21301 agcctggcct ccaggccgcc gacgattctc atgatggtcg gattgcaggg agccgggaaa
    21361 accacaacct ccggcaagct ggcccgtcta ttcaagagtc agggcaaacg tgtgttgctt
    21421 gtggcggccg acccgcgtcg ccctgccgca ggcgatcagt tggccagcct cggtcatgac
    21481 ctgggtatcg atgttcaccg atttgatcaa gtcgatgcct cgcgcgctga tgttgtccgt
    21541 atctgtcagc gcggagtgga gcgcgcccag gaacaaggct acgacctcgt ggtcctcgat
    21601 accggcggtc ggttgcacgt cgatgacgaa ttgatggccg aacttgtggc ggtcaaagag
    21661 gccgtcactc cccacgaggt gttgctggtt gccgatgcga tgaccggtca agatgcggtc
    21721 aatatggctg gtcagttcga ccggcaggtc ggtctcaccg gggtgatcct gacgaaagtg
    21781 gaaggtgacg ccaggggcgg cgcagttctg tctatccggg ccgtaaccgg gaagccgatc
    21841 aagtttctgg gtatgggcga aaagctcgac gccttggagc cgttccatcc ggaccggatg
    21901 gcctcccgaa tcctcgggat gggcgatgtc ctttcgttga tcgaaaaagc gcaggagacg
    21961 ttttcgcggg aagaggcgga agccgcgcaa aagaaactca cgagcagtac gtttacgttg
    22021 gaagacttcc gctctcaact gggacagatg aatcggctgg gatctttcga gcagatcctg
    22081 gggatgttgc cgggcggaca gaaattgaaa gatctcgcga acagcgggtt gccggaaaaa
    22141 gaaatgaagc gggtcgcggc catgatcgat tccatgacca gccgcgaacg acgcgatcac
    22201 acgttgatca atggcagcag gaaaaaacga attgcacgcg gcagcggcac gagcgtgcaa
    22261 gaggtcaacc gtttgatcaa gcagtttttg caggcgcgaa aaattgccaa ggtcatgtcg
    22321 ggcggagcag gtgggcggcg tcagttggcc cagatgcttc ggggaatgtg accggagcag
    22381 ggtaagagct gcgtactcac tggggaagaa gagcgtgtta accgagcaga cccagccatg
    22441 gctgagggct cattattgta ggaggttcta agtggcggtt catttacgat tggcacggac
    22501 gggacgacac aagcgaccga tgtatcgagt cattgcagcg gattcacgta agccgcgcga
    22561 cggccggttt ttagaaatcc tcggcatttt cgatccactg aagaatccgg ccgtgccggt
    22621 attaaagtcc gagcgggtgc tttcgtggtt gcggcacggc gctcagccca cgacgacggt
    22681 gcgaacgctg ttgaaacggc acggcgtctg gaaggaattc gagactgaga agagtgcgaa
    22741 ggctgaaaag ccgggaaaag acgagaagag caaaaagtag gcggagctca tgacgccgct
    22801 cgatcaggca gagcttgtga ccattggaag gatcgaacga tccttcgggg tgcgaggcga
    22861 agcgcgcgtg cggtccctca gcgatgtgcc ggggcgattt gaggcgcttc gcgaggtgac
    22921 cattgttgcg tcgagcggca agagtcttgt gacccgggtg acgcatgttc ggtcaggtgg
    22981 gccgacattg atcgtggggt tcgaagcgtt cacgacgccg gagcaggtcg ccgagtttcg
    23041 cggtggactg ttgcaggttc cgcgtggaga ctcgccggcg ctgccgtcag atcagtacta
    23101 tcaatgcgat ctgatcggga tggttgtcca ggatgaagcc ggaaccgtgc tggggcagct
    23161 ggaagaggtt ctggctatgt ctgataatca ggcattcctc gtacggcagg atggaaaaga
    23221 actgctgatt cctgcggcaa aacaaatcgt gctgtcggtg gatgtcggaa aacgggtgat
    23281 gaccgttcgg ctacccgaag ggttcggaga tctctaatat gcggtgcgcc gttctgacat
    23341 tattccccga tatggtggca ccggtgcttg gccagagtat tctcaagcgc gcccaggaaa
    23401 agggattgct ggaggtttcc gttcagaatc tgcgggacta tacgttcgac cggcataaaa
    23461 cggcggacga tgtgccgtat ggcggtggcg cgggcatggt catgaaagcc gaaccggttc
    23521 tccaggccgt cgatgcgttg cgcgcccggt atcaggcgtc tcatcccgag accgccttgc
    23581 gggtgatcat gccgtctcca cagggacggc cgtttacgca agcgcttgcg cagtctctgg
    23641 cgaaggatga ccgtgtgctg gtattcgtct gcgggcatta tgaaggcatg gatgagcgtg
    23701 tcaatgtggc gctgtgtcct gaacagatct ccgtcggcga ctatgtgttg accggtgggg
    23761 agttggcggc gatggtcatg atcgacgccg cggcccgctt agtccccggt gtactcggtg
    23821 atgcggcgtc agcggccgaa gagtcattta ctgaagggtt gttggaatat ccacattaca
    23881 ccaggcctgc ggaggtgcgc gggatggcgg ttcctgaggt gctggtatcg gggcaccatg
    23941 aggtgatccg cttgtggcgt cgcaaagaag cgttgcgcaa cacctatctg aagcggccgg
    24001 atcttttgcg ggatcgcgag cttggattag aagatcaacg gttattgagt gaagtgatgc
    24061 aagagagtct agtacaggta cctgttcgtt agaggaggag agagattatg aatcggctag
    24121 agcggatcca acgatcctta accaaaaaaa cggcgcccaa gttcgagatc ggggataccg
    24181 ttcgagttca cgtgaaggtc gttgaaggtg aaaaagaacg tatccaggtg tatgaaggaa
    24241 cggttatcgc cagaaaaggg acgctgaata gcgaaacctt caccgtccgg aaactgtcgt
    24301 ataacgttgg agtggaacgg accttcccga tccattcacc cagtgtggcg aaaattgacg
    24361 tcgtccgcca gggacgagtc cgccgcgcca agctgtatta tttgcggacg aagaagggta
    24421 agttcgccaa ggtggaagac cgcgagttca aagtcgaaag caaggcgcag gctgcagcga
    24481 aggtcgaagc tgccgctgcc gcaaaggcct gacaaggccc ttcgtatacg ctcaggacag
    24541 aggcacacgt ttggttggta gcctgaaccc gacgacaggg ggacccaccg aggaatttga
    24601 ggtggaggct cggtcgcgcg ggtaccggca tattgctggt ttggacgaag ccggacgtgg
    24661 gcctctcgcc ggtcccgtgg tcgccgcggc cgtattattg ccgagacggt gccgcctacc
    24721 gggactcaac gattccaaac agatcatcga agcggaccgt gtccggctat tcgatgaaat
    24781 tgttcgacgg gcaatcggtg tcggcgtcgg cgctgcgact gaggtggaga tcgatcgcct
    24841 caatattctc caagcctccc tattggcgat gcgtcgggcg ttgatggcgc ttcccctgca
    24901 accggacttt ctgctcctgg atgcggtcac gctctccggc ctctcaattc cccagcgtcc
    24961 catcatcaaa ggtgatgggc tgtcttgttc catcgccgcc gcctcaattg ttgcgaaggt
    25021 cacgcgcgac cgcctgatgg tcgaatatca tcggtggtat cctcaataca attttgcaga
    25081 gcataaaggc tatgccacgc cggagcatct tcgtttgctc cgcgagcatg ggccctgtgc
    25141 aattcaccgg cgaagtttcg cgcctgtaca acattgcttt gcagaggatg acacggtctc
    25201 cctttcgctg tcagatgaga gtgagagcct gttcgactga tgcgtgatcg acgccgtctc
    25261 ctcggagatg aaggggaagg tcaagccgaa gcctatcttc gccgacaggg gtatcgcatc
    25321 ctgggccgca atgtgcgctc ccctctaggc gaattggatt tgatcgcgga cgacgccggc
    25381 actctggtat ttgtcgaagt caaacgccgg cgatcagggg cctatggcgg cgcgatcgaa
    25441 gcggtagatg cccgcaaacg tgccaaattg attcaactgg catctcacta tctcgcacag
    25501 catcgattgc acgaccgtgc ttgtcgcttc gacgtcgtat tgatccagga cgacgccgga
    25561 tccggcgcag ccgtgcagca tattgcgaat gcattcgacg tcgcagggga tgatctgcga
    25621 tggtaggatg ccgttgagtg tctgaagtgt tgcgccgggc ctgtgccatc ccaggcggaa
    25681 agggccgacc tcgtgctgca ttctgaccgc taccgttgac accattcatg attcaacttt
    25741 ttcacgtttc aaaatactac gaccgccgcc ccgcgctttc cgatgtcaca ctggaaatcg
    25801 agaaaggcga atttgtcctc ttgatggggc ccagcggtgc gggaaagtcg actctgctca
    25861 agctgttgat cggggcggag cgcccggaag agggccagat tctcgtgcag ggtcgaagtc
    25921 tctcgaaact gcgtccgtcc gaaattcccg tcctgcgtcg taaggtgggc attgtcttgc
    25981 aggatttccg gcttctaccg aaaaaaaccg tgtttgagaa tgtttcgctg ccgttgttag
    26041 tccagggtgc atccacgacg gaaatccggc gcaaagttgc agaggcgttg cgggctgtcg
    26101 gtctggatca taaaaaagat ctgtttccgc ccggcctgtc aacgggggag caacagcgca
    26161 tttgtatcgc ccgcgcgatc gtcaacgggc cgatcatgct gctggccgat gaaccaaccg
    26221 gtaatctgga ccctgagtta acgagcgaaa tcatcgagct gttcaagtcg atcaatgcac
    26281 gggggacaac catcattgtc gcgacgcacg atccgaatgt tttggcgcag gtcaatcgac
    26341 gggtgatcac cttggaacac ggaaaagtgg tgtcccggga tcagggggca tcgtgagacg
    26401 cctgctgtat cttttgcgag aggccgtggc caacgtcttg accaatcgga ccaccaccct
    26461 ggtcgcggtg gcgacgaccg cctttacctt tgcctgtgtc ggggtatttt tgctgctcta
    26521 cgtgaatttg agaaccatgg cctcttcgct cgaacaggat attcaggtca tggtgtatct
    26581 gcaggatgac ctgactgagc aggcgcgaag cgaaatagaa caacaattga agattgatcg
    26641 agcaatcgcc tcgttgacct ttgtctccaa ggaacgggcg ctcgctgatt ttcaggccca
    26701 atttccgtcg gagtcgcgtc tcctgcaggg gctcgggcaa aacccgctcc cggcctcatt
    26761 cgttgtcctg atggccccgg agtctcgttc ctccgacgcc atgcggcgat gggctaatcg
    26821 gacccaactg attcccggtg tcagccaggt ccagtataac caggagtggg tggaagccct
    26881 ggctggcatt gtccggtaca ttgaggtggc ggccatcatt gtcggcgtaa ttctgtcggc
    26941 cgcttcggtg accattatcg ccaacacgat tcgtctggcc ctgtactcca ggcgagagga
    27001 aattgagatt ctgggtctga tcggggcgag taccacgttt atccgtgtgc cctatttgct
    27061 ggaaggtgcc gcattgggtt tgtgtggcag cgccctttcg ctggtgatct taaaaggcgg
    27121 attcgaattg ttccggcatc aaattcattc tgccacgcgg tttctggggg tcgatgcgct
    27181 cctgacgttt ttttcgttcg acatgtgcct ggtgctcatg ctggtggggc tgttcctggg
    27241 atgcgcaggg agttttctct cgctgttgcg ttttggcgag gggcgggcat gacgcgcatt
    27301 cagtgggtat gggtggtgtt gagcgtccac ctggcattgt cgacgagccc gccgcctgca
    27361 catgccgaac gcaaggattc gtatgccgac aagatcgagc aggaaaagaa aacgctggag
    27421 aaattacgcg gaaccattgt cgagaagcgt aagaaggccg acgaggccga gaaaaagcgc
    27481 gagtcgatac tacaggggct gcaatcgctg gatgagcgtc tcgtgcgtta ccggcaagaa
    27541 caccaggata ccgtcagaaa actcaaaaaa aaggatgtgg aaatcgagca gatgagcgtg
    27601 cagctgagcc gtctgtccga gcggatcgat gaacggcagg atgccatcgc cgccaggctg
    27661 cgggttcagt atgtcgaggg aaggttttgg caactgaaga cccttctcgc cgccagttca
    27721 tccggcgact ttcagcgccg gtttcggtat ctctccgccg tgacgcaacg ggaatatgag
    27781 atcgtggagg gctatcggaa ggacgccgaa cggatcgccg aagtggaacg gagccgtgag
    27841 gaggccaggc agggtattct cctttataag atcagcacgg aagaaaagct ggctcaaatt
    27901 caagggctca agaagcagaa acgcgtctat cttgcgaaaa tcacacagga aaaggagtcg
    27961 catgatcggg cggtcgagga gctggagcga tccgcgagcc gagtcgacag tttattgaaa
    28021 gagcttgaga cccggcggcg ggccgccttg gcaaatcgtc ctccctccgc cggagcaggg
    28081 cttcgggcat tacgagggac gctcttgtgg ccgacggacg ggcaggtggt ctcctacttt
    28141 ggccgccaga agcatcccac cttcaacacg tatgttcaac gaaaagggat tgagattcgc
    28201 gcagcagaag ggagtattat acgatcggtg cttgccggtc aggttgtcta tgcggactgg
    28261 ttgaaaggat acggactcgt tataatcatg gatcatgcca acggggtgtt ttcactctac
    28321 gcccacgcat ctaaaatctt gacgtcggtg ggcgcccgaa ttgaagccgg gaatgcgatc
    28381 ggtgaaacgg gcgacaccgg catgacaggt gaaaatacgc tgtattttga gctgcgcgag
    28441 ggggcggaac cggtggatcc cctggtgtgg ctgtcaaagc ggtaggcctc ggaaggaagg
    28501 aacccacgta tggaacagcg gcggagccgg cggtggttat atcttttgct gatggtgaca
    28561 gtagcgctgg gagtcgggat cgtgctggaa aagggtttgg agcggaccgg ccacgcgtct
    28621 gaaacctatg aagagctgcg caccttttcc gaagtgttga cgcaggttca aaaacactat
    28681 gttgacgaca cgaaggtgaa agacctcgtg caaggggcga ttcgtggaat gctgtcgacg
    28741 ctggaccccc actccgcgta tatgacgccc gagatgtata aggagatgca agtcgagacg
    28801 aagggtgaat tcggcggcgt cggcattcag atcggcgtga aggaaaatcg gttggccgtg
    28861 atatctccga tcgagggaac tcccgcgcac agagccggga tcaaggccgg tgatttcatt
    28921 accaaggtga acgacgaacc caccaaagac ctgacgttga tggatgcggt gcagaagatg
    28981 cgggggccga agggcaccaa ggtgaaccta acgattcagc gggacggcac cgcggatccg
    29041 ctcgcctttt cgttggttcg cgataccatc aagattgaaa gcgtcaagtt caaggtgctc
    29101 gataacacga tcggatacgt gcgattgacc cagttccagg aagccacagg aagagatctc
    29161 gctcgggcat tgaagcaatt caaagagcaa aaggtccagg ggaccatcct cgacttgcgg
    29221 aacaaccccg gtggattgct gacggcggcg gtcgatgtat cggagcagtt tgtcggaaat
    29281 ggaaaactca tcgtgtatac aaagggacgc gaggggaaaa aggacgagtg gttctccaag
    29341 accaaggaga cgctggagga ttccccgatg atcatcctcg tcaatgaagg ctctgccagc
    29401 gcatccgaga ttgttgccgg ggctttgcag gattggggac gggcggtgat cgtcggaacc
    29461 acctcgttcg gcaaagggtc cgtgcagacc attctgccgc tcggcgatgg ctcagggctc
    29521 cgcctgacca cggccaagta ttacaccccc aaggggcgct cgattcaatc gaccgggatc
    29581 accccggata tcgtcgtgaa actgcagacg cagactgtcg cgaaagcggg cgacaaggat
    29641 gcgaaagaag ctgagccgaa gacggcgaag gctccggcct ccgggaaaga ctcggcttct
    29701 tccaagccag cggatgaacc ggcgcataaa aatggcgcgg cctcattgaa cgatgcaggc
    29761 ggggatatct ccgtggaaga tgatgttcag ttgcaaaagg cggttgaatt gctgaagacc
    29821 tggaaaatct tcaaggagct tcgaccggcg gcgtaagagc gtcagaggca cgctgccggc
    29881 ggatggccgc cagcaggtca tcctccgatc ccgaaaatcc tggttgcgtc ctcaagaggt
    29941 gggacgcaac cagggattcc aaatccgcta acgagcgaat attcagccgg aaatagagat
    30001 agctcaccag cgcgtcagac aggcccggca atgaggccca ttcagcgact tccttcggta
    30061 acggagttct gagttcttca taggcgcgaa tcgttcccgt ggtgagaaac tcttcgattt
    30121 ttcctgccag gtccttgcca attccgggaa tctcctgcaa ctgacgacga gccgccaaat
    30181 ctgtcaggtc tccggtgatc atcaggatgg cgtcggccgc tttacggtag gcgcgcaccc
    30241 ggtaggggtt ggcccgttgg gctgccagta ggttcgccat ggcgcgaaaa atctgggcga
    30301 gttgattgag acggtcatcc atcgtgaaga gtggtatcga gcgtgatggc catattgtgg
    30361 agggaatgcc tgtcaaacgg caaggcctat cgcccttggt tgacaaccgg ttttcccgtt
    30421 tcataggatg attcgacaca ttcaggagcg gttgtgacga tccaggtgaa tggggaatct
    30481 cgcggcatcg gtgacggaca tacggtcgcg gcgttgctgc gcgacttgga cattcgcgct
    30541 gaccgagtgg ctgtcgaatt gaacctggag atcctggacc gcaaggattt cgaaactcgc
    30601 ggtctctgcg aaggggaccg agtggaaata ttgagcttca ttggcggcgg agcggaatag
    30661 ggatggatac gatgaatgat cggttggtga tcgccggcag ggaatttcaa tcgcgtctgt
    30721 gggtcgggac ggggaaatat aaagattttg ccgagactaa aaaagcgatc gatgcttccg
    30781 gcgccgatgt ggtgacggtc gcggtgcgcc gcgtgaatat cacggatcgt tcgaaggaaa
    30841 acctcctcga ttatctggat ccgaagaaat atatcatcct gccgaatacg gccggttgtt
    30901 acacggtgga agatgccgtg cgatatgctc gattggcacg agcggccggc gtctctgatc
    30961 tggtcaagct ggaagtgatc ggcgacgaaa agaccctctt tcccgatacc gcaggattga
    31021 tcgaagcggc gaagattctc atcaaggaag gattcatcgt gttgccctat acgaacgatg
    31081 atccgatcgt tgccaagaaa ctggtggaca ttggatgccc tgcggtgatg ccgctggccg
    31141 ccccgattgg ctctggtctc ggcattcgaa atccatacaa cctgaaaatc atcatggaaa
    31201 cggtcaaagt cccgatcatc gttgatgccg gggtgggaac ggcttcggat gcggcacttg
    31261 ccatggagta tggcgctgac gcggtcctga tgaataccgc cattgccggc gctcaggatc
    31321 cgatcgcgat ggcggaagcg atgaaatatg gcgtgtgggc cggtcgattg gcgtacaagg
    31381 ccggccgtat tccgcgcaaa ctctacgcga cggccagcag ccccatcgag ggcatgctgt
    31441 aaacatttct aagttgtgcg tgacacgttg cgagctggaa gtcgatcatt ccacgtgtga
    31501 gttgatgcgt cacgtcagcg ttcatcactc tgcattcacg atgtcctcct gatgtctgct
    31561 gtcgatttcc gactgtacct cgtcacagat cgtcatcaaa ccgccgggcg tccgctcgga
    31621 tcggtcctgg cacgcgccgt tcgtgcgggg gtgcgagcaa ttcaactgcg agagcgcgat
    31681 ctgcctgttc gcgacctgca tgcactggca acggaacttc agcgggacct gccggatgcc
    31741 cgtttgttca tcaatgatcg cgtcgatgtg gctctggccc tgcgtaccgt gggtgtacat
    31801 ttgcgcgaga gcagtttgcc ggcgggagtg gcgcgatgtc tgctggggcc ttcgcaactg
    31861 ctggggactt cggtgcattc cctcgacggt gtgatcgtgg cggaacagca aggagcagac
    31921 ttcgttgtgt tggggccaat ctatgacact ccgtcgaaac gcgtatacgg gccgccgctt
    31981 ggcatgcgaa ttctcgaaca ggctgcgcag aaggtacgta ttcctatctt tgccatcggc
    32041 ggcatcacag ctgctcgcac gcgcgaggtg cgacaagcgg gggcattcgg cgtagccgtg
    32101 ttgtcatcga tcttaagcgc ggtgaacgtc gagcaggcga ccgaagagtt gcttgctgcg
    32161 atccagatgg agtcgtgatg ccgcacacgc acgcgtgatg ttgaacacgc atacggtcgt
    32221 ggcgtcgccg ccgctcctgg tgcagttgac caccctatag gcggatgata gaatccgaaa
    32281 cagtgctgat gatcgtcatg gattaggatt ttcatcaatc cggcgagtgg ggcgatggcc
    32341 gattcaaaag ggagttcagg ccgactccgg tccttccgtg attccgtcaa gcgcctgact
    32401 cggtcacttt ctgacggaga tggagccgtg acgcacaaga acgacgaaca tgcccgcgaa
    32461 gtggaaaaac ttcgcaccca aatccagtcg atggaggagg agattcggcg gctctaccag
    32521 tcacgctacc aactcgagca aacgacgaag caaaacgaaa agctcgtcgc caccctccaa
    32581 gaagccaaat cacaaatcga aaccttgcgg gccgaagtgg agaagctcac ggctccgccc
    32641 tcgacctacg gcattttttc cagtctggat accgatggaa ccggaaacgt gtatgtttcc
    32701 ggtcgcaaga tgaaggtgag ccttcatccg tctatcaagg cgaaggaact gcgtaaaggg
    32761 caggaagtca ttctcaatga agccctcaac gtcatcgcgg tgcgcggttt tgacgtacag
    32821 ggtgaagtgg tgcgtttgaa agatgtcctg gaaggcaacc gcgcgctggt gaccttgcac
    32881 ttcgacgaag agaaggtggc cgagctcggt gaaccgttgc tgagtgagcg gctcagtgtt
    32941 ggcgatcacc tcttgtatga tccgcgctcg ggttgcgtca ttgaaaagct gccgaagtcg
    33001 gaagccgagg aactggtgct ggaagaggtc ccggatgtgg actatgaaca catcggcgga
    33061 cttcagcggg aaatcgaaca ggtacgcgac gcagtggaac tgcctttcct gtacccgcat
    33121 atcttctcca actataagtt gagcgcaccg aaaggggtgt tgctctacgg ccctccaggc
    33181 tgcgggaaaa ccctgatcgc gaaagccgtg gccagctcca ttgcgaaaaa gctggggcat
    33241 ttaaaagacc ggcaactacg cagctatttc cttcatgtga aggggcctga gctgctgaac
    33301 aaatatgtcg gcgagtctga gcggcaggtg cgcgaagtgt tcaagaaggc caaagaacgg
    33361 gctgatgatg gacatcctgt cattgtattt tttgacgaaa tggacgcctt gttccgcaca
    33421 cgcgggaccg gtatttcgtc ggacatcgag tcgaccatcg tgccgcagtt tttgtccgag
    33481 atcgatggcg tcgaacgcct gaccaatgtc atcgtcatcg gcgccagtaa tcggcaggac
    33541 ttaatcgatc cggcggtgct gcgtgcaggc aggttggatg tgaaggtgaa ggttggccgg
    33601 cccgacgcga cggcggcccg ggatattttc tccaaatacg tgtcgacaga tcttcccttc
    33661 gccgaggaag acctggcacg gcatggcggg gatgcccgag cacttgtcga ctccctgatg
    33721 aatctcaccg tcgatgccat gtatgccacc accgacgaaa acaaattcat tgaggtcacc
    33781 tacgcaaacg gcgaaaagga agtgctctac ttcaaggact ttgccagcgg cgccctcatt
    33841 gagggcatcg tctcgcgagc caagaaattt gccgtcaaac gcgccatcgc tcaagagggg
    33901 agcggcttgc gggccgatga tctgattcga gcgattcgcg aagagttcaa agagcacgaa
    33961 gacttgccga acacgacgaa tccggacgat tgggcgaagg tcgccgggaa aaaaggcgag
    34021 aagatcgtcc atctacggac catcagcggc gggccgtcgg aggcacgcca gatcgaaacc
    34081 gtcagcacgg gccactacct gtaggcgcgc gatatcccat gaatgacact cattcgcaca
    34141 atctgtcccg tgtcatcggc acggagacag aatttggtat tgccagccgg gatccgaatg
    34201 cggcggaccc tgtagcgaac tccattcatg tgatcgggta ctatccaaac ttgcccgcgc
    34261 cccaggccgt gtgggactac gaaaatgaaa accccttgct ggatgcgcgc gggttcgagg
    34321 tcgatgggga acgcgagcgt ccggggccgg actacaatcg acagctcaat aaggtcttgg
    34381 ccaacggtgg ccggttgtat gtcgacggcg cgcacccgga atattccacg cctgaatgta
    34441 ccaatgccag ggaggttgtg gcctttgagc gggtcgggga acgtatcatt gcgcaggctc
    34501 tggccggaat cacggaggcg cgcggccgcg atcaattcgt tctgtataag aacaattcgg
    34561 atggcaaagg caacagctac ggctatcacg aagactatct cgtctcgcgc gccgtatcct
    34621 tcgatcgaat cacgcaggtc ctcacgccgt ttctggtgac gcgtcccatt tatgccggct
    34681 caggcaaggt cggcgccgag aatcagaccc cttccgtcga ttatcagatc tcgcagcggg
    34741 ccgatttttt cgaaacgctc gtcgatctga acaccatggt ccggcgaccg atcatcaata
    34801 cgcgagatga accgcattct gattcgacca aatatcggcg tctccatgtg attgtcgggg
    34861 atgccaatat ggcggagttg tctacgtatc tcaaagtcgg tgccctttcg atagtcctcg
    34921 atctgctgga ggctggagcc gatcttcccc ggataagcct ggccgaccct gtcaatgcca
    34981 tcaagcaagt gtcccgggat acgcaggtga aggagagcct caagctgacc gacggaagcg
    35041 cgacaacggc gattgccgtg cagcgggcct acctcaaggc ggcgcagagt tactatgcct
    35101 gccacgaact gaatcaggtc acgaaagaca tcctggtgcg ttgggaggat gtgcttgatc
    35161 gcctcgaacg ggatccgcgt ttgttggtgc gggagttgga ctgggtggcg aagcgctatc
    35221 tgattgagtc ttacatggac cgcaagtcct gcggatggga cgatcctcgc gtgcggttga
    35281 tggatctgca atatcatgat gtccgtccgg accggggatt gtattacacc ctggaacgca
    35341 gtcacatgat cgagcgggtt gtgctggagc atgagattgt ccgcgcggag acaaatcctc
    35401 ccgtcggaac gcgagcctat tttcgcggcc agtgcgtcaa gaaatacccg aatgccgtct
    35461 acggggtgag ctggacgtcg gtgctctttg atgtcgggca gaacaagatc aagcggattc
    35521 cgttaatgga ccctctgcgc ggtacagaag cgctgacggg tgagctcttc gcccatgcgg
    35581 aaacagcagc tgcgctgctc gaaaaactat caacctgact gcgaccatca catgaatcgt
    35641 atgccctttc ttccacacca cgacggttcg agtttcttcg attttctcac ccagtatcac
    35701 cccgaattaa gaccaggcgt acacgatctg gcgatgcccc gcgctacgct tcctctagac
    35761 ctcggccgtc caggcgccat cccggtgccg cacggaacga cggtgctggc attgaagtat
    35821 cgggatgggg cgattattgc cggcgaccgg cgggcgacgg aggggtttca gattgccgac
    35881 cggcggattg aaaaagtgtt ccgcatcgat gagtattccg ccatggctat tgccggtgcg
    35941 gccggcccct gtattgaaat ggccaagctt tttcaaaccg agctggagca ttacgaaaag
    36001 ctggagggtg tccagctctc ctgcgagggc aaggccaaca agctgggaca aatggtgaag
    36061 gccaatctcc cgatggtgtt tcaggggttg gtcgtgatgc cgctctatgt cgggtacgat
    36121 gtgaagcgaa aagaaggtcg tatcttcaaa tatgacataa cgggtgggcg gtacgaagag
    36181 tccgatcacc attccatcgg ctccggcggt aaagatgcac gcaataccat gcgggaacat
    36241 taccggtcgg ggcttgatga agaggctgcg ctccgtgtgg gcctgttggc cttgtacaac
    36301 gcggcggatg aagacgtggg aacggggggg cctgatctcg tccggggaat ctatccaacg
    36361 gccaaggttg tcagcgatgc cggtattacc gatgtgccgg aatcacgtgt gcgtgcccta
    36421 tatgaagcca tgatcgcaga acgaaggaga tcgtaatcaa tgccgttgcc ctactacgtc
    36481 tcgcctgaac agatgatgca ggataaggcg gagtatgcca aaaaaggcat cgccaaggga
    36541 cgttcgatta tcgccttgga gtatgtcgac ggcattgtgc tggcggccga caatcccagt
    36601 tcgtctctcc acaaggtgtc ggaagtgtat gatcgtatcg catttgccgg ggccggcaaa
    36661 tatagcgaat tcgagcatct ccgcaaggca gggatacggc acgccgatct gaccggctat
    36721 atgtacagcc gcgaagatgt gagtgcccgg actctttcca acgcctattc acaaagcctg
    36781 gggacggcct tcagcacgga cgtgaagccg ctcgaggtgg aaatccttgt ggtgcaggtc
    36841 gggggcaacg gccaggccaa cgaaatcttt cgtatctcgt tcgacggcag catcgtggat
    36901 gagaaaagtt ttgcggtcat cggcgggcga tcagaagccg tgcagcaata cctgcgcgaa
    36961 catgcgacgc cgcagccacc gacgctcaaa gatggcctcc ggcgctgtct ctccgccctt
    37021 gagcaggtcg ccactcagaa gattccttct gaaaacctcg aggtcgcggt actggacaga
    37081 aaccgtcagg ggcgaaagtt ccgacggctc accagcgccg atatctccca tctgcttgcc
    37141 taacgttcat ccctcaacat agtccattgc ggtatggccg gtcattgtcc ggtaatttcc
    37201 gatcgcgcgt ggacgacgaa tgctcaccgg agtctgttga agccacccgc gtcagggtgt
    37261 atcctgacag gagtcgcacc gagggcatcc atctccatgc aacaacgaat tttcggcctt
    37321 gaaaacgagt atggtctgat tttctctccc aacgggaaga tctaccttcc gatggagaaa
    37381 gtcctgggct acatctttga agggctgatt ccgaacagtt ggccgtcgaa cgcgtttctg
    37441 gtcaacggcg cgcgtttcta tcaggatacc ggctgccatc ccgaatactc cacgcctgaa
    37501 tgcgacaata ttctggacct ggtgattcac gataaggccg gcgagcggtt gctggaagcc
    37561 tgtttaccgg cggcggagga gcggttacgg gaagaggggc tctccggcga aatctatatc
    37621 tttaagaaca ataccgactc gctcgggaat acgtatgggt gccatgaaaa ttatctcatg
    37681 cgccgggatg tggatttttg gaaggtgacc gagcagctca tcccgttttt tgtgacccgg
    37741 cagatctatt ctggcgcggg caaggtgttg aaggtttccg gcaagccgca gtacttcatt
    37801 tctcaacgcg cgcagcatat ccacgaaaag acgtcctcct cgacgacctc ttcacgcagt
    37861 atcatcaata cccgcgatga gccccatgcc gatgcggaga aataccgccg gctccacatc
    37921 atcgtgggcg attccaacat gtcggaatac gccacctatc tgaaggtcgg cactgccacg
    37981 ctggtcttat cgatgatcga atcgggtttc tccgtgacgg gcatggaact cgaggatccc
    38041 gtcaaagcca tccgcgaaat ctctcgcgat cctacgctga acaagaaggt gaagctggat
    38101 gacgggcgac agatgaccgc gatcgaaatt cagcgcgtct atgtcaagcg agccacagag
    38161 tttctggccg cgcaggatca cgatccggtt ctggacgacg tgctggacaa atggatctcc
    38221 gtgctggatc ggctggaaga cgacccgatg cagctcgtgc atgaggtcga ttgggtgatg
    38281 aaaaaacatc tgatccagtc gtatacggac aaaaaggatt gcgggtggga tgatcccaga
    38341 gtgttcctgc tggatttgca atttcatgac gtgaaacgga cacgtgggct gtattattta
    38401 atggagaatc gtggcatggc agcgcgggtc gtggaggaag aggcggtaca acgagccatg
    38461 tcggtcccgc cacagactac cagggcgaaa gtccgcggtg acttcatccg gtttgcacgc
    38521 gccaagaatc ggtcgtatac ggtcgattgg acctatctga aactgaacgg gtattgggaa
    38581 gagacgattc tctgtatgga cccgttcagc gcagtcaatc ggcgcgttga agaattattg
    38641 tcgcaggtct ccggtgcgcg attgtaccga tgagacgggt tggtggagtg aatcgagccc
    38701 acaccatgtc gttcctcgat cgctttctca tcatctcggt cgtgctgctc gccagcgtgt
    38761 ttcctgttga gctcttcccg aacacggggc ctgttcctcg cggcggagac gggcaattca
    38821 gcggcaagca gggtcaggtg gtcgtggtga ccgtcccgga actcaaggaa gccaccagcg
    38881 tcaaaggaaa gttcctcggt cgcactgtaa ccctgttccc gaatcccacc gccggaggca
    38941 cgggctacat cggattgctc ggtatcgatc tgcaagatga accgggaccg catgagctga
    39001 ctatcgatgc gcaactaggc gagcaaacca gacatttttc gtatcaggtg cttgtcgtaa
    39061 aggagaagtt tgccgtcgag catctgaaac tgccgaaaga taaagtcgat ctcgatgaga
    39121 aagcgaccgc gcgctggaag gctgagcaag agcaggttcg aaaggcctta gcggaagagt
    39181 ctgccatgcg actctggcag gcggggttta tcgagcccgt tcacggtaaa cggaccggca
    39241 tttttggcag tgtccggatt atgaatgggc aacccaggaa tccacacaac ggagaagata
    39301 tcggcgcccc gatggggacc gacgtcatgg ccagcaatga cggggttgtg cggctcgtcg
    39361 tcgatcatat tttttcgggg cgaggcatct ttgtcgatca tggcctcggt ctctactcga
    39421 tgtactttca cttgtcggaa gcgctggtca acgaagggga tctggtaaag gccggtcaag
    39481 tgatcgggaa ggtgggcgcc acagggcggg cgaccggtcc gcatctccac tgggggatga
    39541 aagtcaacgg cgctcgggtg aacccctacg cgctcctcga attgcccttt ccgcagaatc
    39601 ccgccgcgga ccgcccggta ctggcggcgc cagcggtccc cgctcaggaa gaattagctc
    39661 ccacagcagt cggtggtgac ggccggtagt ccgtccctcc agtacgcccc tcgtcgactt
    39721 cgtggctctg ataacccttc aggttgagtg cgacgacgta tccctgcagc ttccttgtcg
    39781 agtctgtgat tccagcgctg aggccacggt cttggtcagc gactcgggcg tatacggttt
    39841 cggcaaaaag gccacctggg catcgaatgt tttgaggagt gtttcatcgc gccctgaata
    39901 gccggacgtg aggatgactt tgatgtccgg ttggagggtt tgcagttcgt gagccagctg
    39961 ggcgccgccc atgcccggca tcaccatatc tgtcaccagt agcgcaatgt ggcgtccctg
    40021 ccggcgcacc atttccagcg cttcctctcc ggagcgggcg gccaggacat gatagtgctg
    40081 ggcaacaagc acggtatggg ccagacctct caccatgtcg ttgtcttcca ccagtagaat
    40141 ggtttccgac tgttcgctcg tgaccttggg ctgcgtcacc gcgcgaggag cgccggtgtc
    40201 ttcctccagg cgcggcaggt aaatcgtgaa gctggtgcct tttcccacct cgctcgtgat
    40261 ccccacggtg ccgctgcttt gtcgaacaat cccgtagacc gtcgacagtc cgagccccgt
    40321 ccccttgccc aattccttcg tggtaaaaaa cggttcgaag acatgggcca atgtttcggc
    40381 gctcattcca caccccgtat cacgaacgga cagggttaca taaggcccag gaggaagagg
    40441 atcaggccag aatggatcgg aatgggagat catcacttcc ccggtttcca attccagccg
    40501 gccgccgtgg ggcatggcat cccgggcatt gacgaccaga ttcatcacga cttgttcgat
    40561 ctgcccagga tcggctttga ttcgtcccaa cgtcgggtcc agggtcagga cgagatgaat
    40621 gtcttcgccg atgacccgtt tcatgagttc gacgatatcg ctgacgcgct cattaaggtt
    40681 cagggactgc cgttcgacga cctgatggcg tgtgaaggcc agcaattggc gcgtcagtcc
    40741 ggtagccttg ttgccggctt ctctgatttg cgccatgcct cgttgtgccc gggacgtcag
    40801 gcccggttcg tccatcaaca gttcggccca actattgatg atggtgagga ggttgttgaa
    40861 gtcgtgtgcc acgcctcccg ccatccgtcc cagggcttcc attttctgcg cctggcgcaa
    40921 ctgtcgctcc agatctttcc ggttgagaaa ttggctgatc atcgcgccca gctcggcaat
    40981 tcgctccatg cgggacacgg cagggtgcag gacttcgacc gtaaaaaact ccatgatgcc
    41041 caggatctct ttgccggtgt ggatcggaaa ggcgatcgct ccatggaggt ccgcctctcg
    41101 cgcggcatca tgcgcgggga agtcggcctc attcacaata tccggactga atgccggggc
    41161 gtccgttttc catgcccgtc cagccattcc catgtcctgt cttaggaaca aggtgcgata
    41221 ggcctgggcg aagtgctcgg tctgttgccc tgagggatgc cagagtgcat tgcagcgtag
    41281 aaccgagtgt tgagtatcga caagccacag cgcacccaat gtccagccct gaagttcgca
    41341 catcgcgcgc agaatttttg gcacggcctc attcaacgac acagattctt ccagcgcgag
    41401 gcccacggtg agttgaacgg catgcatggt ttctgcttgc cggcggtcgg aaatgtcggt
    41461 ttttgccccg atcatccggt agggggtgcc ggcatagttc cggagcacat agccatggtc
    41521 gatgacatac gcataggtgc catctgctct gcgaaaacga tattccgccg tccacaagtt
    41581 tttgtcggac tccagtgtat ggctgaggcc cgcaagaatc gattggcgat cctccggatg
    41641 gatttggtct gcccacgcaa tccgtcccgc ttcccgcgcc ttcgcccctg agccgtagac
    41701 ggtttgatag gcatcgttga accaggtctc cccggacgta atgtcccaat cccagatgcc
    41761 gtctctggtg gcaagacacg tgagatgaaa tcgctcttcg cttttccgca acgcctgctc
    41821 tacgcgatgt cgccccatgg cataccggat ggcgcgtttc agcccatcac ccgttaatcg
    41881 ccctttaacc aaataatcct gcgccccatg cagaagagat ttctccgcca cgaggtcatt
    41941 gtcgagtccc gtcagtacaa tcacggccgc ttcatgagct tgggcccgca cgcggacaag
    42001 cgtgtccatt ccctggctgt ccggtagcga cagatcgacc aacacggcgt ccggagagcc
    42061 ttcggcaagt ttcagcaggc cggcttccag acggtccgcc caggtgagca ggacatcctg
    42121 cggtgagtgc tcgcagagtg cctcgcgaat caatgccgcg tcatcttcat tgtcttcgat
    42181 cagcaacacc tgcatgtgtt acctcttgag cgtcggaacc ttggagacga gcgtccaata
    42241 gagttcgaat tctttgacga ccgcgatgaa gcgggtgaag gtgacgggct tgcgaatata
    42301 ggagcaggcg cctttctcga acgaacgcac aatatcctct tcccgctcgc tgaccgtcag
    42361 gatgacgacg gggatggcac gaaatcgcgg gtcggatttg atttcctcca acacctcgag
    42421 ccccgtcttt ttcggcatat tgatatcgag gagcaccatg ccggggagcg gacattggct
    42481 gaacgagcct tcaccccgga ggtaggccat cgcctcagcc ccatcccgga cgacggccac
    42541 ccggttggtc atgttgacgg tttcgaacgc ctcgcggatg aggagaatgt catcttcatt
    42601 gtcttcagcg atcagaatat cgaacggcct catcgtcata gtcctacctc tttcacaacc
    42661 tgccggttga tcccgaatgt gataaaaaag tccgatcctc ctccctcacg gggtaagacc
    42721 caggcgcggc ctccatgccg ctccgccact tgccggacga tggccagacc ggctccggtt
    42781 ccttcgattt cccgtcccac cgctcgccgg aacaactcaa agatgcgctc acgctggtcg
    42841 gacggaaccc ccggtccgcg gtcgcgtatg accaggccgg aacattccgt cccctcagca
    42901 tcaatctcgg tatggggagc aatctcgata tccggaggtc tcccgtcagc gacaaacttc
    42961 aaggcattcg ccaccagatt gtagatgccc tggacggccc aggtcgtatt gacccggagc
    43021 cgcggcaacg gcgtgaacac cgtgatcgtg gccccgctct ctttgatgcg gctttccagc
    43081 cgtcgtaaca cctcttggac gagctgatgc gcgtcgatct cgtcgaccgg tggttccatg
    43141 cgctgggcgc gggagaggtc caaaatgtcg gtgagcaatt ggtcgagccg ctgtgtggcg
    43201 cgtacgatgc ggcgaagaaa atcctgtcct ttctcatcaa gccggctcgc atagcgttcc
    43261 tgcagcagaa ccgaaaaact ttcaatcgcg cgcagcggtt ccttcagatc gtgcgaggtc
    43321 acatagagca gggtctcgag atccttattt ttcttcagga tcatcaattc ggcttggcgc
    43381 cgttcggtaa tgtcccggtt cgtttcgagg accaggctgc gaccccctgt tttcatcaat
    43441 ccccagcggc tttccacgag cacctcccgc ccgtctttgg tgcggtgatg taactcgccg
    43501 gtccaggctc cggtcgcttc caattcagca caaatggtgt cgaaagggca gggataagta
    43561 attttcagca gtgtgtggct gcttcgtccg atcgcttccg cacgggtgta gccgtacaat
    43621 tgttcgcatc cccgattcca atccaggatg ccccgctcaa aatcccaggt aaaaatcggg
    43681 tcataggaca gttcaatgag tcggcgctgt tgactcaaga cttcctcggc acgtttgcgt
    43741 tccgtgatat cccgggcgat cgtcgacacc gccgtcacct ctccgttggc atcgactatg
    43801 ggcgacacgg tcaaggacac ctggatgggg tggccgtctt tatggagacg cacgctctcg
    43861 aattgttgga tgcggattcc cgctgtgatc tgctcgatga ggatggcttc ctccgcccgg
    43921 cgctctggcg ggagaatccg ggagaccggc tggccgataa tctcttcgct aggatagccg
    43981 aacatcaact ccgcgctttt gttccaggac aagatccgtc catccaagcc cttgctcaga
    44041 acggcatcgg atgatgattc cacgatggaa gccagtctgg cctggatttc ctgcgcttgt
    44101 ttgcgatcgt ggatgtcggt gtaggtcccc acccatttta gcaggtggcc atcgtgatct
    44161 cggatgggcg tcgcatgcgc atggaaccag cggtagatgc catccgcccg cttgaagcgc
    44221 agttccgtat ccaggggctg aatatgagcc acggagtggt gccattgctc ggtaattctc
    44281 gggcggtctt caggatgaac gtgctggagg catccggtgc tgaagggctc gtctgcagat
    44341 cgtcccgtat aatttgccca tcgagggctg agatagtcgc aggtgccgtc agggaggcag
    44401 gtccagatcg gcagcggcag ggattcgaca agttcgcgaa accgggtttc gctcgcaatc
    44461 agtgcctgat gcatctgttt gctttgtgcc agctcctgtt ccaaacggct gttggcttcc
    44521 tgaagcgcag ccgtgcgttc ttcgaccaag gcctcgagag actgatgact cagacgcagg
    44581 gcctggtcag cgcgtttgcg ttccgtaata tcaatcagcg tggagcggct tgacacgaac
    44641 cgtccgtcag agtcggtcac ggccgtggc
//
LOCUS       DAOQEW010000006        42090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_175_length_42090_cov_8.782681, whole genome shotgun sequence.
ACCESSION   DAOQEW010000006 DAOQEW010000000
VERSION     DAOQEW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42090
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175_length_42090_cov_8.782681"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLS35_01445"
     CDS             <1..836
                     /locus_tag="PLS35_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNI17339.1"
                     /translation="TSLEEIVMGLRTRRDWYGADTKVVTEEISKTSRLVSKITGMVIQ
                     PNKAIVGANAFAHTSGIHQDGLLKDKTTYEIMRPESVGLEQVKLVMGKLSGRHAFRQR
                     LEDLGYKLTEDEVNHAFERFKRLADQKKEIYEEDLEVIISEEVAKMSERVVLKSFQVE
                     SGTNKTPKATVELEIDGKVVSHSGTGDGPVDAVYRTIAAMTQTKSKLLMFGVNAITGG
                     TDAQGEVSVRLEEDGRTVSGHGADTDIITAAARAYLNALNKLAYFAAKQAEGIHKVSL
                     I"
     gene            939..1301
                     /locus_tag="PLS35_01450"
     CDS             939..1301
                     /locus_tag="PLS35_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNI17340.1"
                     /translation="MFSTHLQQCPEFLAGDHTRLRELLHPGKAALELGYSLAHGLLDP
                     GKQSLWHRLKSSEVYYFIAGRGVMKVEEASMPVQAGSVIYVPPGAKQSLINTGTESIE
                     FLCLVDPAWKAEDEAVVE"
     gene            1320..2396
                     /gene="leuB"
                     /locus_tag="PLS35_01455"
     CDS             1320..2396
                     /gene="leuB"
                     /locus_tag="PLS35_01455"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNI17341.1"
                     /translation="MKAKIAVLAGDGVGREIVPEAVKVLKTVAERFGHTFEFQAADVG
                     GHAIDKVGVPLPAETLTLAKHSDAVLLGAVGGPKWEGLDYSIRPERALLGLREQLGLY
                     ANLRPAKLYPMLADASTLRREVIEGIDMLVIRELTGGIYFGKPKGVEKMPGGGERGFN
                     TEVYTTEEIRRIAVVAFEAARKRRKKVMSVDKANVLESSELWRRVVTEVQKDYADVTL
                     SHIYVDNCAMQLVRNPRQFDVLLCNNIFGDILSDEAAMLTGSIGMLPSASVGAKVSLF
                     EPIHGSAPDIAGKNIANPIATIASGAMMLSYAFQLDKEAAAIEQAIVKTLEQGFRTKD
                     IHAEGMRLVGTTEMGDAIVKNLPA"
     gene            2400..3614
                     /locus_tag="PLS35_01460"
     CDS             2400..3614
                     /locus_tag="PLS35_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17342.1"
                     /translation="MTISATPSAMIATLAGNGEPGYAGDGGPAAVASLNEPKGLCIDR
                     EGNVYIADSENHVVRRVDRKTGLITTVAGVGPGGTGGAMMAPEPVAMPPQEEEDPFAD
                     TSGDSTKAYTQVTDLSGTVRYVTGGRLTIEQESGDGGPARRARLNFPSAVAVDRAGNL
                     YIADTMNHRVRKVDAKTGIITHVAGTGQARYSGDGGPAVQAAINEPTGLAVTDDALYI
                     ADQSNNRVRRVDFATGVITTAAGDGSAAYSGDQVPAVQASLAGPSGVAVGNDGLLYVA
                     DTFNSRIRSVDPATGQITTAAGDGGTYRYQGPEEPASPSLSRPAGLAVDYDGNVYITD
                     SDSHLIRVWNGRTKTITRVSGTGVAQFGGDGGNALAGSLSYPFGVAVDAAGTVYVADT
                     FNHRIRVLTATA"
     gene            3625..4644
                     /locus_tag="PLS35_01465"
     CDS             3625..4644
                     /locus_tag="PLS35_01465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNI17343.1"
                     /translation="MLKKKQAYTVAVLGATGAVGKESLEILEERNFPIETLRLFSSKR
                     SAGEVLTCQGKEYKVEELTEASSFAGVDIAFISATDAISRDYGARLGAAGIVVIDDSA
                     VFRMDPDVPLVVPEVNAAALQSMKRGIVAIPNCTTTPLVMALKPLRDVAGIKRVVVTT
                     FQSVSGTGSAAMDELVDQTKALISFQDVKVQVYPYQIAFNLLPQVGSFGDGGDCSEEV
                     KIVQETRKILDMPTLRVTATTVRVPVLRCHSEAINVELERPMKANDARAAIAEMPGVI
                     VYDDPVKKLYPMPFDVSGKDEVYVGRVRVDESITNGLNLWVVSDNLRKGAALNAVQIA
                     ECLIQ"
     gene            4648..4902
                     /locus_tag="PLS35_01470"
     CDS             4648..4902
                     /locus_tag="PLS35_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNI17344.1"
                     /translation="MGAWSGIGRLFIVAGLALAGVGILLTLLERWSGAGAAFGWIGRL
                     PGDLSITRERFSLYVPITTSLVLSILLSVVLYILSWIFRR"
     gene            4941..6092
                     /locus_tag="PLS35_01475"
     CDS             4941..6092
                     /locus_tag="PLS35_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNI17345.1"
                     /translation="MALAGIVFAQSASAESIRVLLVQEAPFVDVRSDGPLALVMETGD
                     TKTLHGPIHLTARADGLHVDGRRILGGQVLIRSLRHNLTLVVGKEGGSAGTPLPISGT
                     VRVSRKGHAVAVVNQVDLEEYVKGVVPSEVSSAWHPEMLKVQAVAARTYALYNKMLSA
                     ARDYDVMATIQDQVYRGRTGVDHRVEEAVESTRGIVVTHQQAPIYAAFSSTAAGPTED
                     AVNVWANKDLPYLKGVECPFDLESPYYQWKASVKIEQLEKNLRHQGFAVGTIATITPI
                     AFSRAGRVARLRILHSGGETLLRGEDLRKAAGYTVIPSTQFEVESIGAEVVFAGYGAG
                     HAVGLCQWGAKELAELGYSYNSILQYYYPGTELRNSALSQLMMPVIPTP"
     gene            6092..7132
                     /gene="queA"
                     /locus_tag="PLS35_01480"
     CDS             6092..7132
                     /gene="queA"
                     /locus_tag="PLS35_01480"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNI17346.1"
                     /translation="MLLSEFDFPFDPALVADRPVLPRHDARLLVMDRSSHARMHRRVA
                     DLPSLLQPGDLIVVNNTKVMPARVPAQTRSTGKILDLLFVQDLGQGLWEVLIKGRFKP
                     GAFIDFPGGGCGEIVERSQARTTVKVTGVTSVYDLMHNTGTMPLPPYIKREPSQDDQR
                     WYQTMFAQQEGAIAAPTAGLHFTPELVETLTGKGMELAEVTLHVGPGTFRSVKSDRIE
                     EHRMLAEQVEVTARTVEQIRLAQKRGNRVVAVGTTVVRALETAGRGGRGIQPFQGPAE
                     LFITPGFEFQVIDALVTNFHLPRTTLLMLVSAFVGLEPLRAAYEEAVAERYRFYSYGD
                     AMFIARGLSAAT"
     gene            complement(7125..7823)
                     /locus_tag="PLS35_01485"
     CDS             complement(7125..7823)
                     /locus_tag="PLS35_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNI17347.1"
                     /translation="MIKLLREAAHDYPWFLKSIMGTLALAFVITMGWWGFGDQSGNVV
                     ASVGDQVVPLDEYRRAYENTYRFYKDKGQNDIKDEFLKQFVLEQLIDNRMWLHVAKEM
                     GLSVSDEDLRKAIMQRTEFQKNGNFDPESYRRLLAANRLTPASFEAMEAKDILTNKAR
                     LVIMDAVALTPSEYAEAQTLVSREGESDPTKAAIAKERIFQNLLFQKQQRALMAYSES
                     MKSKVPVKIHKELM"
     gene            7827..8444
                     /locus_tag="PLS35_01490"
     CDS             7827..8444
                     /locus_tag="PLS35_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17348.1"
                     /translation="MRLFSAGFRGFYSGDDSKGKRKKCAESGCEKIATLFVQVKEICY
                     SVEELGRFPVAEEAIGRSPVELGVYPKAQVLNRFIAKMIDLLIVAAVSKLVPPIGVLA
                     GLAYILLGDGFGGGRSVGKRLIGLQTIVPRTRDPAGFRESIIRNLPCGLAQLAFEIPY
                     VGWIGWGAVLSLEGLLVIGNEQGRRLGDEIAKTQVLESGQLDVSD"
     gene            8493..9542
                     /locus_tag="PLS35_01495"
     CDS             8493..9542
                     /locus_tag="PLS35_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNI17349.1"
                     /translation="MAFMSDMFGWFSNDLAIDLGTATTLVYVQGKGIVLNEPSVVAVE
                     KKTERVMAVGADAKRMLGRTPGNIIAVRPMKEGVIADFEKAEAMLSHFIQKAHNRTAF
                     VRPRIIIGVPSRITQVEQRAVRDSAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDIGGGTTDIAVISLGGIVYSESVKVAGDRMDDAIMNYIKKKYNLLIGEHMAERIKFE
                     IGSAYPFEERKTMMIKGRDLISGIPRTLVVDDAEVREALQEPIGTIVNAIKVALENTP
                     PELAGDIIDRGIVLTGGGSLLKGMDTRFREETNLPIITVDDPLTSVVLGVGKILDELD
                     LLRKVSVMSQCSTSR"
     gene            9561..10442
                     /gene="mreC"
                     /locus_tag="PLS35_01500"
     CDS             9561..10442
                     /gene="mreC"
                     /locus_tag="PLS35_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNI17350.1"
                     /translation="MAISRSTYGTRRLAIVLFACLLVALFLLPSQSQRLLQYVGGPLG
                     QVLSLPLAAFSSVDHGIADMWNGYVALQTVREENRQLHRDIELLKGQNNQLRESMTAV
                     QRYEALLNFKQQSPSQTLAAQVIGRDATNWYRGMILNKGENDGVRTEMGVITPAGVVG
                     RVVKTNATSSVVLLVTDPNNAIAGVVQRTRDEGIVEGTSHGRARLKYIPLLSNVQAGD
                     RVVTSGLTGAFPRGLAIGSLIQVEKSEGDLFQSAEIEPEVDLSKLDEVLIITAPYEDA
                     DAAQKLLQAIHGDRKKP"
     gene            10439..10939
                     /locus_tag="PLS35_01505"
     CDS             10439..10939
                     /locus_tag="PLS35_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17351.1"
                     /translation="MKFLLYLLLALVVVPLQTTLLHYASVFGVRPDLGLIAACLVGFL
                     GGELDGLILGLILGCFQDMLSAGDLWINVVTKGGSGFLAGLAGRHMAHITPAVMTAGL
                     AIISCLSSAVFLYSMNPAGLDEVWLGVRSTVLVQAGLDAALGAGLYLLFRQRWSDDRM
                     VTEGAL"
     gene            10981..12864
                     /gene="mrdA"
                     /locus_tag="PLS35_01510"
     CDS             10981..12864
                     /gene="mrdA"
                     /locus_tag="PLS35_01510"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNI17352.1"
                     /translation="MATVGFNDSDLLDLQRRLVILRVGLLLVVALLALRLWHLQIREG
                     PYYRDLSENNRTRSVVLEPARGLIFDRNGVLLANNVPSFALYVTLEDVKDRQALVGQL
                     ASLLALDPEVIQKKLSTGKGGKLQPRKVKDRLTLREATLIESHRLDLPGVMIQVESQR
                     NYPAGAVAAHLLGYVGEVSADQLEKPDFADLHQGSIVGQYGVEKWFDRQVRGRAGQKS
                     IEVDALGHEKRAIVSDKPLAGDDLYLTIDAKLQKVAEDLLGDESGAIVALDPTNGDIL
                     AMASRPGFDPNVLSKELTNKQWVEIVQNERRPLNNRATQGQYPPGSTFKVVMAAAALE
                     SNTVTPSTMVRCNGGYQFGNRLFHDWKQGGHGSVDMNEALIHSCDVYFYTVGQRMGID
                     TIAEYAKQFGLGQETGVELPSERVGIVPSTAWKQKARNQPWYPGETISAAIGQGYVTV
                     TPLQMASVIGTVANDGVSIKPRLVQAVMNRTTGERAEFPPTVRGKVAVKPATLALIKS
                     ALADVVTKGTATRAKSPLVTIGGKTGTAQTAALRTGPEKDIPKKFRDHAWFVAFAPVE
                     APRIAVAVLAEHMGHGGSAAAPLAKDVIEAYVKAYPQVLQGAPASGRAKSPAVPVDTR
                     PKT"
     gene            12864..13982
                     /gene="rodA"
                     /locus_tag="PLS35_01515"
     CDS             12864..13982
                     /gene="rodA"
                     /locus_tag="PLS35_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNI17353.1"
                     /translation="MIDRVIDSRGLDSFDLRFMGLIAVILSVGVLSIYSVTHSQDTAF
                     PFYLKQLVWILLGTVAFLVMYLSDYHKIARLAYPTYAVILIMLAVVLFMGKSSRGAQR
                     WIPIGPFAFQPSEFAKLVLVLVLANYYSRASRVGWLHRVVLPGLIVLPGLLLILKQPD
                     LGSGLSFLAVYAAMLLMVGVRSKALGIILLLSVMLFPFVWEVVWASLHDYQRERVMAF
                     VDPDYDPGGKGYHALQSRIAIGSGELSGKGLYGGTQSQLKFLPEGHTDFVFAVYAEEW
                     GFVGVLVLLALFIALIWVSLEIAARAKDTLGALLAAGIVAMLCFCVVVNIGMTAGMFP
                     IVGIPLPLVSYGGSATIMTMASLGLLLNVKRRRLTLFY"
     gene            14125..15636
                     /locus_tag="PLS35_01520"
     CDS             14125..15636
                     /locus_tag="PLS35_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNI17354.1"
                     /translation="MGVEIAISIAREETRVAVLDNGVVTDLYGDRAKDQDYVGNIYKG
                     RVAKVLPGMQAAFIDIGLEKAAFMHVSDLSIDVEPGDTLVEGDDEDNKDSEVPKPKRQ
                     SSKPIEELLSEGQELMVQISKGPIGTKGSRVTTYVSLPGRYLVFMPTVEHIGVSRRIP
                     RDEERARLKEIMKRARRPGFGYIVRTVSEGVKEEELRSDVEFLHVLWQDVLTKRDQQP
                     APSLLHSDLSLSFRVVRDLFGRRVDRLLIDSREEYNAIKGFVQRFSPEQTSRIHFYDK
                     EESLFDYLGVEQEIARAMSRKVWLKSGGYLVIDHTEAMTVIDVNTGRFVGKRDQEETI
                     LRNNLEAAREIAYQVKLRGIGGIIIVDFIDMEREKNRDKVYHALVDAMSSDKARTRIS
                     RVSDLGLIEISRERVREDLLRSLSEPCHYCDGRGYTKSPMSVAYEIFREVRRLGHEIE
                     QQRVVVGAHPAVALLLQEQEQPGVEELERRYSAKILVTPDDRLHLEQFDLVVM"
     gene            15746..16867
                     /gene="dprA"
                     /locus_tag="PLS35_01525"
     CDS             15746..16867
                     /gene="dprA"
                     /locus_tag="PLS35_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNI17355.1"
                     /translation="MVNSLSLRPWLMLRAIPGVGDAILLKLVQAMGSPDAVLSATQAS
                     LEDVGCRVPLIDAIRRGPDAQAVKQLDRELAAVQHRQITVLTYLDPQYPAPLKTIPDP
                     PPLLYVQGTLLESDRQAVAIVGTRKVSSAGRVLAEELACELAEMGFTIVSGLARGVDA
                     AAHRGALAGKGRTLAVMGCGLDRTYPADHAALRQEIERHGAVLSELPLGSAPHSYHFP
                     RRNRIISGLSLGVVVTEAAVESGSLITARLAGEQGREVFAVPGFVKAENSRGPNSLLK
                     DGARLVESAQDILDELLPQLDAAFRERLGDRARGARPAMQLGPEEAMVYDAVSVLPQS
                     VDDVIRRSGLAAAQVTAVLLSLELKNCVRQLPGNEYVRL"
     gene            16963..19302
                     /gene="topA"
                     /locus_tag="PLS35_01530"
     CDS             16963..19302
                     /gene="topA"
                     /locus_tag="PLS35_01530"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNI17356.1"
                     /translation="MAKSLIIVESPTKARTITKYLGRGYSVMASVGHVKDLPTSKLGV
                     DLEHDFEPQYVTIKGKSKVLADIKKKAQQVDTVFLAPDPDREGEAIAWHIAQELHGKG
                     KKKDGKVYRVLFNEITESAIKRALKSPGEIDMKLVQAQQARRVLDRIVGYQGSQLLWK
                     KVRRGLSMGRVQSVAVRLICDREQEREKFRAEEYWSIVALLRGDNPPAFEAKLHSING
                     QDADIGTSAQAEQILNAVQGKAFVVQSIERKEKKRNPVAPFITSRLQQEAARKLHFTP
                     KKTMTLAQQLYEGVEIGAEGPMGLITYMRTDSPRISQEATEDARLVIRERFGEEYLPA
                     TPNVYKTQKAAQEAHEAIRPTMAARDPESIRQFLEQDQYNLYKLIWNRFIASQMVPAI
                     LDVTRVDSSLIGTPERYVFRTTGTVVKFPGHTAVYLEGTDKELPSQKPKSEQESEDES
                     DRQLPVLREGESLHLVTQEAQTLPGLISKQHFTQPPPRYNEALLIRELEEKGIGRPST
                     YAAIISTIQDRKYVEKVEGRFLPTETGRTVNDFLLKGFPDLLDTEFTSHMEEQLDEVE
                     EGTKPWVSAVREFYAPFVREMELAQSIPGPKDIVEPPTNLPCEKCGRMLEIKWGRNGK
                     FLACPGYKEDPPCKNTQNFEKLPDGTIKIVPKLEETTDEKCEKCSSPMVVKSGRFGKF
                     LACSAYPECKTTKAIALGVKCPQPGCGGDLVQKRTKKGRNFYSCSRYPECEFALWDRP
                     INKPCPKCQAPFLIEKVSKQAGRSVQCRNEEECGYREAG"
     gene            19610..20086
                     /gene="bfr"
                     /locus_tag="PLS35_01535"
     CDS             19610..20086
                     /gene="bfr"
                     /locus_tag="PLS35_01535"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNI17357.1"
                     /translation="MKAKQGVIELLNKVLTADLTAINQYFVHAKMCANWGYERLHHKV
                     RERSIDEMKDADELISHILYLEGVPNVQRMNTVHIGETVPEQLKLDLKAEQEMLALLS
                     EGVVHCTKVADFTTRHMFEDMAKDVDEHIDWIETQMETIKQIGLENYLAEQIKKDS"
     gene            20121..20600
                     /gene="bfr"
                     /locus_tag="PLS35_01540"
     CDS             20121..20600
                     /gene="bfr"
                     /locus_tag="PLS35_01540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNI17358.1"
                     /translation="MKAKEGVLDILNKVLVSELTAVHQYLLHAALCKHWGYARLHEHF
                     SHLAQEEVSHSSGLIDHILYLGGSPEMGRLDAVAAGKKVSELFSADLAFEREDADALR
                     QGIAHCAKVGDFTTRHRLEDMIIDTEEHIDWFETQLRTIEQVGEDRYLAEQLTADKS"
     gene            complement(20708..21268)
                     /locus_tag="PLS35_01545"
     CDS             complement(20708..21268)
                     /locus_tag="PLS35_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNI17359.1"
                     /translation="MMSGKSSTSNISFDRLHSFSSELRVQSPQWADRLEQVRTEQDLG
                     DLICDLFNLSHAINPETQPPPIPHTQPSRIAAFVNENLHRGVTLKVLANFLGYSEKYC
                     SDLFCRIMGEPFSKYLRRQRVERARALLITTRQTLTEIASALGFSDQFAFSHFFKRAT
                     GQSPMAIRLTIARQQRQTIQGLQQTS"
     gene            complement(21326..22081)
                     /locus_tag="PLS35_01550"
     CDS             complement(21326..22081)
                     /locus_tag="PLS35_01550"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNI17360.1"
                     /translation="MHRSTRPIPTTSQLIQDLATQLSALNTLQLSPESLEAVRVIQLE
                     RLARRIETVIPGHFGHGERTAHYALLLADRLRLTKHQRLDLHYAALLHDVGLLMMPGH
                     LLGAPAPHTLNDYALIQSHPREGAALLTPYTFLQVAARLIAHHHERWDGAGYPYGLRG
                     EYIPASARILAIADVFDSITSRTNSWDSALRTLHASAGSQFDPAFCVTFIELLRNHDH
                     YRAYQSSDLSSDAMHSQSSPEHVPEAYMTGSGI"
     gene            complement(22187..22705)
                     /locus_tag="PLS35_01555"
     CDS             complement(22187..22705)
                     /locus_tag="PLS35_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17361.1"
                     /translation="MRNFNTRFRLRDSDRHIRLVYTFFLLLMLAGFTFSFFWAHSMTG
                     LSPQGIADHYRGSNATFGEPMSFRELAEITHFHLFTMPVVFMILVHVLYLTNASNRLK
                     IVTTWLSFGGVILDLLSPWLISYVSPVFVLTMLTGDTFMTAGFFIMFVIPLHEMWILK
                     QPLMAGKRGEHA"
     gene            complement(22718..23395)
                     /locus_tag="PLS35_01560"
     CDS             complement(22718..23395)
                     /locus_tag="PLS35_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNI17362.1"
                     /translation="MMPLLLLLTLTGAFALAPLNAQAERVWDNELKRYLTEQEMSLAE
                     VFLTEEEAVKLMFPKSERVRRELLNLPQDKKALIESRIGWKFPEESFEVYIGETGTHI
                     DGYALVQNTIGKHKPMTYMVGVDAHGLVSNVELLTFREARGSEVRTKRFNVQYEGKSV
                     LDPVRINKDIINISGATMSVRSMTAGIKRVLVLVDEFYLKPQGRGSDTVTAQKTEKGF
                     FKSIFGN"
     gene            complement(23392..23853)
                     /gene="bamC"
                     /locus_tag="PLS35_01565"
     CDS             complement(23392..23853)
                     /gene="bamC"
                     /locus_tag="PLS35_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNI17363.1"
                     /translation="MQRAHIFIAAAAFLTIGCASLSGSHEQSYSCSQDVVWDAAMDTM
                     KSYSITSQDKAKGVIETGWLEMDGKERSYGIFGRTGFGNRERARMSLALKTSNGAVSV
                     SLLETRQRWHSRGGVTSQATKWWPVEPSEEATAEVTTRLNEKLKQQGCTPL"
     gene            complement(23917..25395)
                     /locus_tag="PLS35_01570"
     CDS             complement(23917..25395)
                     /locus_tag="PLS35_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI17364.1"
                     /translation="MKIRAILGALVLASLPAMPALADNMTPEEIKKLVDEAVEKRLQE
                     HERREGAGDHRETVGAPGNEPGIGSLPEVGKERRTENQAALSFGSTGSGRLVYAKPFV
                     AAPKAIVGGYMDIQYRSHRNGVLESGMGSGTTNGFDQQRFVPFIYADITEHVKFAAEI
                     EIEHGIREDATQGAEIGLEFAHIDYLVKEPFNLRAGILLVPIGKFNLLHDSPLNDLTD
                     RPLVSRLVIPSTMAETGAGFYGTLYPGRTSKLDYEFYVTTGPCGYNGDGSPRIREEDG
                     TRRSRQRKCPSEDGLDINNGKAVSGRVAFSPMLGVEVAGSSYFGNASPLSYNPLSITA
                     IDWTFQRGPFELLGEAAWAYARGNSRAVQGATFATPGSLLSGLNTLNSFAAPPQRMQG
                     FYVQANYHFMPAFLTALSPKRFGEGSTFTAVFRYDRVNLNMDNRGENSGELEQFSFGL
                     NYRPIEDAVFKASYQYMPKSFNPNTGQRIHDSAFVISAATYF"
     gene            25971..27071
                     /locus_tag="PLS35_01575"
     CDS             25971..27071
                     /locus_tag="PLS35_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNI17365.1"
                     /translation="MKLSINSHPLRAFLFMFVNDAVLRRGFALAIRGSLGLLLLLGLG
                     CAGGNLGSSPILVKRTQMHMGTWVSITAVAPDREAAQYAASAGFQEIHRLEELLSTWI
                     KDSELSRVNGAAGKEAVSVSPDTMKVLQASVAMARLTGGGFNILVGPAVEAWSVLDRQ
                     QIPSESELENIRPLTNLNALQLSQAQGTVYLAKPGMRVDVGGIGKGFAADMAVEAMQK
                     AGATAGVVALSGDIRTFGQMPDGTTFPFGIRHPRREGTVLAFVDLQNEAISTAGDYER
                     YFERDGVRYHHVLDPVTLQPARDCQSVTIVAKDGLTADGLDTGIFVMGPERGLALVES
                     LPGVGAVIVDRDGKVWVSSSLKGRVRIKDGVD"
     gene            complement(27152..27412)
                     /locus_tag="PLS35_01580"
     CDS             complement(27152..27412)
                     /locus_tag="PLS35_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNI17366.1"
                     /translation="MPRIEALKEYPVDARLKHVEGRVVVRIVIQEDGQIVSAAVAKSS
                     GHDTLDQAALETVRKISPIALTQPLEQSPVTLHVPIRYQLGP"
     gene            complement(28099..28473)
                     /locus_tag="PLS35_01585"
     CDS             complement(28099..28473)
                     /locus_tag="PLS35_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNI17367.1"
                     /translation="MEREVNQINVIPLVDVMLVLLVIVLTTATFISTGQIPVNLAKAK
                     EAGDHKDVPVVVTLTANGDLFLNDRPVPADGLKSVMLTHPRESLVVVRADKVTLLERF
                     VSVVDEIRGLGFQSVSLEVVRL"
     gene            complement(28457..28888)
                     /gene="exbB"
                     /locus_tag="PLS35_01590"
     CDS             complement(28457..28888)
                     /gene="exbB"
                     /locus_tag="PLS35_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNI17368.1"
                     /translation="MDVLKNAVEYGIIGLLIALSVWSVAVAVERWLYYRRVNLAQFTD
                     VQTFEMALTKRLVIIGTVAANAPYIGLLGTVLGIMMTFHTMGTSGTMAVNTIMIGLSL
                     ALKATAVGLLVAIPCVVMNNILRRRVAELLTTYKVQHGTRG"
     gene            complement(28988..30136)
                     /locus_tag="PLS35_01595"
     CDS             complement(28988..30136)
                     /locus_tag="PLS35_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17369.1"
                     /translation="MSLVNESITGRQQPASEGIIPNTLDTTVERSAVLELRDVSCAYE
                     PTRPAIEGITFTVHQGEILCLLGPSGCGKTTILRAIAGFERVTRGTIALSGQRVSSPD
                     TMIPTEQRHIGMVFQEYALFPHLRVEKNIAFGLRHLPRTEQQAIVDDLLTLTGLRGLE
                     HRYPHELSGGQQQRVALARALALRPVLLLLDEPFSNLDPDMASRMRQDLHALLRQTKT
                     TAILVTHDHDEAFSMADRVAVLNKGRLEQFDTPEAIYHLPTTPFVADFVGQADFIAGV
                     VTHHLVTTELGDFPNTQHLATGTHVVVMIRPDDIHIVPTKGADARILARQFKGSENVY
                     TIQLPSGQIVHSSESSLSIYQVGTAIALRVVATHTVLFPQPPGSPPVA"
     gene            complement(30133..31737)
                     /locus_tag="PLS35_01600"
     CDS             complement(30133..31737)
                     /locus_tag="PLS35_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNI17370.1"
                     /translation="MLTATRVRAPSSLQWISLFTAAFLVLPTAYIVYVAMTAAPAVWA
                     RLWSTRIPELLGNTLSLALGVALITLVLGVSLAWITVRYDFPGRRIWEWALALPLAMP
                     TYVLAYVYAHLLGMGGPTEQWWQLLFGHEARLPSPQSFPGVTMIMALDTFPFVYLLVR
                     SAIQNLNVSFEEVARACGVSPWATLRRVTLPLIRPAIAAGLALVILYVISDFGAVSLL
                     RYQTLTYAVYQQMTSRYDHTAASILSLLLVVMAIIFLVTERWFRQRSRFYQTSGRYRR
                     STRQHAGPLGTALITSYVVLVFGAAFGIPAAMLIQWSVEAISQGALDTRFLGFIWNSS
                     FLAALAACGAVIIGLPLAYLASRRPSRLNIACLQAAYAGYALPGPVAALAVLVLFTHF
                     APALYGTVVVLIIAYILHFLPVGLQSMEPALQQVTPNVEEVARTLGCTTHQSLWRVTL
                     PLVRNGFVAAWVLMFLQTMKELPATLLLRPVGFDTLAIRVWLEASEEYYQLAAPAALL
                     IVILSLPALMLLVSKDWRGEAQKGVT"
     gene            complement(31753..32784)
                     /locus_tag="PLS35_01605"
     CDS             complement(31753..32784)
                     /locus_tag="PLS35_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNI17371.1"
                     /translation="MASFPRTVFALTLLISALILQEWPAKTADAADKLVVYSGRAERL
                     IKPVLDEFQTKSGIQIELLSSGTTELVNRLQAEGDHTPADVFLTNDAGSLEHARELKL
                     LRPMNMREVERAIPSQFRASDNSWIGLSGRFWVVVYNTTLVKPEQIKSLLDLAQPQWK
                     DKIAIPNSGSEYLQAGVSVIKTTFGDERTKQFLQGLKTNAGSQVYQKSSQIVEAVAKG
                     QVAAGIVNHYYIYRHLAAQPTAPIAAIMTDQQEGGMGAIMNVTGIGVTRASKHVDSAK
                     LLIEFLVAQAGQKLFADLDKEYPLHPDVKADPALVDRHSFRAALVPLARLAELRESTL
                     TLIEQVGLR"
     gene            complement(32876..33307)
                     /locus_tag="PLS35_01610"
     CDS             complement(32876..33307)
                     /locus_tag="PLS35_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNI17372.1"
                     /translation="MSKNLKEMDVLRQHLAKHQLKLTRQRELILTAFLRQEHVTAETM
                     YHQLAKKDPHLGLATIYRTLNLFCEAGIAQARHFGTQTQYDNISHKGHHDHLICTGCG
                     TIVEFENCEIERLQEEVATKNGFVIQTHRLELYGLCARCRH"
     gene            33459..34022
                     /locus_tag="PLS35_01615"
     CDS             33459..34022
                     /locus_tag="PLS35_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17373.1"
                     /translation="MRKSHEMTVDRALLLYVLSLAEPYGLLSDVKLQQLCFLCELQMF
                     GKGLKGFHFEFVRFAYGAFSRDLDNDLTSLRRKERVENFSPSDQVRDETLPLLLKGID
                     GNETNEKVRDLMQAVIATYGPQDVTAIMQSVESVELSTPQQPDFKIPIRDIVFHTTLL
                     VPSRIEAPAEFLLPANHLAKLNVSMGY"
     gene            complement(34156..34542)
                     /locus_tag="PLS35_01620"
     CDS             complement(34156..34542)
                     /locus_tag="PLS35_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17374.1"
                     /translation="MANILIINADSNLKRLIKLALPGMEHFVQYSATLHNASAQPPAS
                     KLDLVVARLVADDDQGPEDLVDLRKAFPSSRIVVTAALHEPALEGEKFQRVVRELQIE
                     YCLLEPIETGAMLHTIQSALTLPRRH"
     gene            complement(34677..35363)
                     /locus_tag="PLS35_01625"
     CDS             complement(34677..35363)
                     /locus_tag="PLS35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17375.1"
                     /translation="MNAFDTLPAVKTELNILLADDHALLRRGLKEFLQDFLTEDGLTP
                     HITETDSAHSAIDQIRTGAWDLVILDLNLPDMPGLEVLRIVKSLQPSLPILIVSIYAE
                     QHYAARAIRAGALGYLTKDTGPQQLRAAVSRILQGGNYFPPPQSEQVSDTMLDRNTPC
                     AHDLPAVLSDREIEVLQWIAQGRRLTEIAEHLNLSIKTVSTYRARLLTKLGMKTTAEL
                     IRYALDQQLV"
     gene            complement(35832..37352)
                     /locus_tag="PLS35_01630"
     CDS             complement(35832..37352)
                     /locus_tag="PLS35_01630"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNI17376.1"
                     /translation="MKTLKTLEPLTNRLLEIRRIQSAASVLSWDQETYMPAGGGAARA
                     EQIATLEGLAHERLVSPHIESLLNEWIDPSTGQAAEHWDEPSRSLLRETWRDFSRARK
                     LPSDFVIRLSRECSLAQQAWVTARAESQFSKFLPSLRTILDLKCEETQYLGYRDSPYD
                     ALLDTYEPGATVGQLTPLFAQLRARLVPLLKRVQASKVRIDDTCLHHSFDHTKQIEFG
                     RLVLVAMGYDFERGRLDLSAHPFTTSFHPTDVRVTTRVFEKDLPSCLFSCIHEGGHGL
                     YDQGLDPRYYGSPLGESVSLGFHESQSRLWENCVGRSRAFWQCFYPILQHTFPQQLAG
                     VPLDQFYAAINRVTPSLIRVEADELTYNLHIMLRVEIEQALIERRAHPEDLPSLWNDR
                     MEAYLGITPAQDADGVLQDVHWSMGAFGYFPTYTLGNLYSVQFFEQAKLELPQLEEQI
                     AAGQLVPLRHWLEQKIHRWGRMFTPDHLARRVTGNGVTPEPFLRYLEAKYGALYRL"
     gene            37437..38087
                     /locus_tag="PLS35_01635"
     CDS             37437..38087
                     /locus_tag="PLS35_01635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17377.1"
                     /translation="MADLVSPNIDAYAAACSLPETPVRRALREETERTMEYARMLVGP
                     LEGAFLHVMTRLVRAERVLEIGMFTGYSALCFAEALPDQGRVITCEVDEDSAAVARRF
                     FAQSPHGRRIEIRMGPALETMAELTGPFDLIFIDADKLNYVNYYRRAMELLSPRGVIL
                     VDNVLWGGDVLTDPPPDDRTAAIQDLNRIVAADPRVTAVLATIRDGIWVITPKSSH"
     gene            38146..39057
                     /locus_tag="PLS35_01640"
     CDS             38146..39057
                     /locus_tag="PLS35_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNI17378.1"
                     /translation="MEKPSASAAYAALTISALVWGGSIVGQKLALGAFSAVETSLIRG
                     IGALAILIPLWWWTEGNRTTLTARDLKVLSLLGLFVFGNHLLTLFGLRYIGASTAGVL
                     IGASPAITALLSSLLVRDIPFNQVAGGCAVSFAGVALVSGVGGDAPIGDHPWLGGTLV
                     LLGLVSWALYSIGGRQVMNRLSPLTVNWTTLLLSLLLQIPLLSTDQKMLVTGTSAVPV
                     SGWLAVGYLIVFATALGQQAWLYGVQGVGPSRAGVFVNLIPVSALLLSAVILGEAIGA
                     REIAGIVLILMGVWLVGRQTEPSKRRG"
     gene            39253..40773
                     /locus_tag="PLS35_01645"
     CDS             39253..40773
                     /locus_tag="PLS35_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17379.1"
                     /translation="MIEHHDTGLSRVLVISTRPDGIITSFNSAAERLLGVGADAVVGK
                     VTPAAFHDPDELRRRRETLAAGLGKPIQDSFGVLVARVGIGRMTEDEWTYLDRSGYRF
                     PVFLSVSAIPDEAGHVLGYCFVAKDRRGQLQAEALLRRQAKLLDLANDAILVRDLQAD
                     TITYWNEGAVRLYGWTAEEALGAYIHDFLSTTFPLPLEEIKQQFLEQGLWRGELVHKT
                     RAGREITVSSRWTLLRDAAGMPSGSLELNTDITEQKRAQKALKDAHEELELRVMERTT
                     DLRDANERLRILSRRLMEIQELERRAIARDLHDEIGQALTAIKLNLRELRTHPNCEPV
                     EDQIADSLEILAQVLQRVRSLALDLRPSLLDELGLVPALRWYVGRQAERAGWQLTFSA
                     DGLTSRPPAEIEIACFRLAQEALTNVVRHSQAKKVEVLLERSSQALTMVIRDNGIGFD
                     RDEVRSGASVGLSGMEERVQLVGGAFSIESTPAVGTEIRAYFPHGVATKGTEDSLV"
     gene            40770..41423
                     /locus_tag="PLS35_01650"
     CDS             40770..41423
                     /locus_tag="PLS35_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17380.1"
                     /translation="MNKIRVLLAEDHTLVRAGFRALLEKLEGIQVIGEVSDGRDALRV
                     AKELDPEVVLMDIAMSGMNGLEATSRMRQECPRTKVLMLTMYTNEEYLKEALRAGASG
                     YLLKDADRPELELAIKTVCRGETYLTPAMAKFTLDAYCRQDDVPMSPLSKLTGRQREI
                     LQLIAEGCSTKQIAHRLDLSVKTVETHRAQLMERLEIHDVPGLVRLAIRTGLVRSDT"
     gene            41633..>42090
                     /locus_tag="PLS35_01655"
     CDS             41633..>42090
                     /locus_tag="PLS35_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNI17381.1"
                     /translation="MKKLRVAVLGTGAFAEACHVPGLQSHPGAEVVVLCGRNSARTRA
                     MADRLGVPEISLDYEQVCARNDIDAVTIATPNVAHALQAKAAFSAGKHVFCEKPLGMN
                     VQEVIEMVRAAERSHAIHQVAFTYRYLHGVQELKCRLLKGDIGEPYHVRV"
BASE COUNT         9006 a        10878 c        12969 g         9237 t
ORIGIN      
        1 acacctcgct ggaagaaatt gtgatggggc tccggacacg cagggattgg tacggcgccg
       61 acacgaaggt ggtgacggag gagatctcca aaaccagccg cctggtgagc aagatcaccg
      121 gcatggtcat tcagccgaac aaggccatcg tgggcgcgaa tgcctttgct catacttcag
      181 gcattcatca ggatggattg ctgaaggata agaccaccta cgaaatcatg cggccggagt
      241 ccgtcgggtt ggagcaggtc aagttagtga tgggcaagtt gtcagggcgc catgccttca
      301 ggcagcggct ggaagacctg ggctacaagc tcacggaaga tgaggtcaat cacgcctttg
      361 aacgcttcaa gcggctggcg gatcaaaaga aagagattta cgaggaagac ctcgaggtca
      421 tcatctccga agaagtggca aagatgtccg aacgggtggt cctgaagtcg tttcaggtcg
      481 aaagtggaac gaataagacg ccaaaagcca cggtggaact ggagattgac gggaaagtgg
      541 tgtcgcacag cggcacggga gatggaccgg tggacgcggt ctaccggacg attgccgcta
      601 tgacacaaac caagagcaag ctgctgatgt tcggggttaa tgccattacc ggcgggacag
      661 acgcgcaagg agaagtctcg gtgcggctcg aggaagatgg ccggaccgtc tccggtcatg
      721 gtgcggatac ggatatcatc accgcggccg ccagggcgta tctgaacgcg ctcaataagc
      781 tggcgtattt tgcggcgaag caagccgaag ggattcacaa ggtcagcttg atttgatccc
      841 aacctgcaag gtagattcct ccgtcctggt cagcacatgg tgttggtcag ggcggggcga
      901 catcttgtat tgccccctat cgtcacaatc ggcacacgat gttcagcacg caccttcaac
      961 aatgccctga atttctggca ggcgatcaca cgagattgcg ggaattgctg catccgggaa
     1021 aggccgcgct ggagctgggg tatagcctcg cccatgggct gctcgatccg ggcaagcagt
     1081 ccctgtggca tcgcctgaaa tcgtccgagg tgtactactt catcgccggg cgcggcgtga
     1141 tgaaggtgga agaagcctca atgccggttc aggctggctc cgttatctat gtgccgccgg
     1201 gcgccaaaca gtcgctgatc aatactggaa cggaatcgat agagtttctc tgcctggtcg
     1261 atcccgcctg gaaggcggaa gacgaggcgg tcgttgaata gttgagagga gagcgcgacg
     1321 tgaaagcaaa aatcgcagtc ctggccggag atggagtcgg ccgtgaaatc gttcccgaag
     1381 cggtcaaggt cttaaagacc gtagcggagc ggttcgggca tacctttgaa tttcaagcgg
     1441 cagatgtggg tggccatgcc atcgataagg tcggagtgcc ccttcctgcg gagacgctca
     1501 ccctcgcgaa acacagtgat gccgtcttgc tcggcgccgt cggtggcccc aaatgggaag
     1561 gcttggatta cagcatccgt cccgagcggg cgttgctggg tcttcgcgaa cagctcggac
     1621 tctatgcgaa tttgcgtccg gcgaaactct atcccatgct cgccgatgcc tccaccttgc
     1681 ggcgagaggt cattgaagga atcgatatgc tcgtgatccg cgagctgacc ggcggcatct
     1741 atttcggcaa accgaagggc gtggaaaaaa tgcccggcgg tggagagcgc ggctttaata
     1801 cggaggtcta tacgaccgag gaaattcgcc ggattgccgt ggtcgcgttc gaagcggccc
     1861 gcaagcgtcg caagaaggtc atgtcggtcg acaaggccaa tgtgttggag tcgtccgaat
     1921 tatggcgacg cgtggtgaca gaggttcaga aggactatgc cgatgtgacg ttgagccata
     1981 tctatgtcga caattgcgcc atgcagttgg tacgcaatcc gcgtcagttc gatgtactgc
     2041 tttgcaacaa tatctttggc gatatcttga gcgacgaggc tgcgatgctc accggatcga
     2101 tcggcatgct gccctcagcc agtgtcggcg ccaaggtgag cctctttgag ccgattcatg
     2161 ggagcgcgcc ggacatcgcc ggaaagaata ttgccaatcc catcgccacc atcgcttccg
     2221 gcgccatgat gctgtcctat gcatttcaat tggacaaaga agcggcggcc attgagcagg
     2281 caatcgtcaa gacccttgag cagggattcc gcaccaagga cattcatgcg gaaggcatgc
     2341 ggttggtcgg gaccactgag atgggtgatg cgatcgtgaa gaaccttccc gcctaggcca
     2401 tgacgatcag cgccacacca tccgcaatga ttgcaaccct ggcagggaac ggcgagccag
     2461 gatatgccgg tgacggggga cctgccgcag ttgcctcgtt gaatgagccg aaagggcttt
     2521 gcatcgaccg cgaagggaat gtgtatatcg cggattctga aaatcacgtc gtacgccggg
     2581 tcgatcggaa aacaggcctg attacgacgg tcgctggagt gggtcctggc ggaacgggtg
     2641 gggccatgat ggcgccggaa cctgttgcga tgccacccca agaagaagag gatccttttg
     2701 ccgacacctc gggggacagc accaaggcgt acacacaggt cacggatctg agcggcaccg
     2761 tccggtatgt caccggggga cggctgacga ttgagcagga atcaggagac ggcggaccgg
     2821 ctcgccgcgc gaggctgaat ttcccgagtg ccgtggctgt ggatcgtgcg gggaatctct
     2881 acattgccga cacgatgaat catcgcgtcc ggaaggtcga tgcgaaaacc ggcatcatca
     2941 ctcacgttgc cggcacaggt caggcgcggt attccggcga tggcgggccg gcggtccagg
     3001 cggcgatcaa tgagccgacc ggactggctg tgaccgatga tgccttgtac attgcggatc
     3061 agagcaataa tcgcgttcgc cgggtggact tcgcgaccgg ggtcatcact acggcggccg
     3121 gcgatggatc ggccgcctac agcggcgatc aggtgccggc tgtgcaggcc agtttggcag
     3181 ggccgagtgg tgtggcggta ggcaatgacg gtctgttgta tgtggcggac accttcaaca
     3241 gccggatacg atcggttgat ccggcgacgg ggcagatcac tacggcggcg ggagacggcg
     3301 gcacctaccg ctatcagggc cctgaggagc ctgcgtcccc aagtctttcc cgtccggccg
     3361 gtctggccgt tgactatgac ggcaacgtgt acatcacgga ttctgatagt catttgattc
     3421 gagtatggaa tgggcgcacg aaaaccatca cgcgcgtgtc agggaccggc gtcgcgcagt
     3481 tcgggggaga cggcggcaac gcgttggcag gcagtctcag ttatccattc ggcgtagcag
     3541 tcgatgcggc cggaaccgtg tatgtggcgg atacgttcaa tcaccgcatc agggttctca
     3601 cggcgactgc gtaaggacag tatcatgttg aagaagaaac aggcctatac ggtggcggtg
     3661 ctgggggcga ccggagcggt cgggaaagaa agtctcgaga ttctggaaga acgcaatttt
     3721 ccgattgaga cgctgcggct tttttcgtcg aagcggtcgg ccggcgaggt gctcacctgt
     3781 cagggcaaag agtacaaggt ggaggaactg acggaggcct cctcttttgc cggagtggac
     3841 atcgcgttca tttccgctac cgatgccatt agccgggact atggcgcgcg attgggcgcg
     3901 gcgggtatcg tggtcatcga tgacagcgcc gtcttccgga tggatccgga cgtcccgctt
     3961 gtcgtgccgg aggtgaatgc ggcggcgctg cagtctatga agcgcggcat cgtggcgatt
     4021 cccaactgta cgacgactcc gttagtgatg gcgctcaagc ccttgcgcga tgttgcgggg
     4081 atcaaacgcg tcgtcgtcac cacttttcag tctgtgtccg gaactggatc agccgccatg
     4141 gatgaattgg tggatcagac caaggcgctg atctcctttc aggatgtgaa agtgcaagtc
     4201 tatccctatc aaattgcctt caacctcttg ccgcaagtcg ggtcgttcgg agacggtggg
     4261 gattgttcgg aagaggtcaa gatcgtccag gagacgcgca agattttgga catgcctacg
     4321 ctccgggtca cggcgacgac tgtgcgggtc ccggtgctgc gctgccattc cgaagccatt
     4381 aacgtggaac tcgagcgtcc gatgaaagcc aatgatgcgc gggcggccat cgcggaaatg
     4441 ccaggggtga ttgtctatga cgatccggtg aaaaaactct atccgatgcc gttcgatgtc
     4501 tccggcaaag acgaggtgta tgtcggacgg gtgcgcgtgg atgaatccat caccaatgga
     4561 ttgaatctgt gggtcgtcag cgacaatctc cggaagggcg ccgcgctgaa cgccgttcaa
     4621 attgccgaat gtttgataca atgatctatg ggtgcttgga gcgggatcgg ccggctgttc
     4681 atcgttgccg ggttggctct ggccggcgtt ggaattctcc tgacgttgct tgaacgatgg
     4741 tccggtgccg gcgcggcgtt cggatggatc ggccggttgc ccggcgatct gtcaattacg
     4801 cgagaacgct tcagcctcta tgttccgatt acaaccagtc ttgtgctcag cattctgttg
     4861 agcgtcgtgt tgtatatcct ttcatggatc tttcgccgct gatcccacat cgtgcccgga
     4921 gtttcctcgc agcgggagtc atggcgctcg ctgggatcgt cttcgcgcag tctgcctcag
     4981 ccgaatccat tcgtgtctta ctcgtacagg aagcaccgtt cgtagacgtg cggtcggatg
     5041 ggccgctggc tcttgtgatg gagacgggcg acacgaaaac cctgcatggc ccgatccatc
     5101 tcacggcgcg agcggacggg ttacatgttg atggccggcg tattctcggc ggacaggtgc
     5161 tcatccgctc cctgcggcac aatctgaccc tggtcgtcgg gaaagaagga ggatctgcag
     5221 gcactccgct tccgatcagt gggaccgtgc gagtgtcacg caaagggcat gcggtcgcgg
     5281 tcgtcaatca ggtggatttg gaggaatatg tgaagggcgt ggtgccatcc gaagtcagtt
     5341 cggcctggca ccccgaaatg ctgaaggtgc aggccgtggc ggcgcgaacc tacgcgttat
     5401 acaacaagat gctcagcgcg gcacgcgact atgatgtcat ggcgacgatt caggatcagg
     5461 tctatcgggg acgtaccggg gtcgatcatc gagtcgaaga ggccgtcgaa tcaacccgag
     5521 ggatcgtcgt cacccatcaa caggcgccga tttacgccgc cttttcctca accgcggcgg
     5581 gcccgactga agatgcggtg aatgtgtggg ccaataaaga tctcccgtac ctcaaggggg
     5641 tcgaatgtcc ctttgatctg gagtcgccgt attatcagtg gaaggccagt gtgaaaatcg
     5701 aacagcttga gaagaatctc cggcaccagg gatttgcggt cggaaccatt gccacgatta
     5761 cgccgattgc cttcagtcgt gcgggaagag tcgcgcgttt gcggattttg cattccggcg
     5821 gcgagacgct gttgcggggc gaagatctgc ggaaggccgc ggggtatact gtcatcccca
     5881 gtacgcaatt cgaggtggaa tcgatcggtg ccgaagtggt gttcgccgga tacggcgcag
     5941 gccatgcggt ggggctgtgt cagtggggcg caaaagaatt ggctgaactc ggatattcgt
     6001 acaacagtat tctgcagtat tactatcctg gaactgaatt acgaaactcg gccttatcgc
     6061 agttgatgat gccggttatt ccaaccccct gatgttactc agcgagtttg atttcccatt
     6121 tgatccggca cttgtggccg accgtccggt actaccccgc catgatgcgc gcctactggt
     6181 catggaccgc agcagccacg cacggatgca ccgccgggtg gcggatcttc catcgctgct
     6241 ccagccgggg gacctgattg tggtgaataa taccaaggtc atgccggcgc gggttccggc
     6301 ccagacgcgc tcgacgggga aaattctcga tctgctgttc gtgcaggatc tgggacaggg
     6361 cctgtgggag gtattgatca aaggacggtt caagccggga gcgttcattg attttcccgg
     6421 cggcggatgc ggcgaaatcg tggagcggag tcaggccagg acgaccgtca aggtgactgg
     6481 tgtgacgagt gtgtatgatc tcatgcacaa taccgggacg atgccgctcc cgccctatat
     6541 caaacgcgag ccctcgcagg acgatcagcg ctggtatcag accatgtttg cgcaacagga
     6601 gggcgccatt gccgccccga ctgcgggact tcattttacg cccgaattgg tggagaccct
     6661 gaccggcaag ggcatggagt tggcggaggt gacgttgcac gtggggcccg gcacgtttcg
     6721 cagcgtgaag agcgaccgga ttgaagagca ccggatgctg gccgagcagg tggaggtgac
     6781 tgctcgtacg gtcgagcaga ttcgcctggc gcagaagcga ggaaatcgag tggtggcggt
     6841 cgggaccaca gtcgtgcgcg cattggaaac ggcaggccga ggaggtcgtg gcatccagcc
     6901 tttccagggt ccggccgaat tattcatcac gccggggttt gaatttcagg tgatcgacgc
     6961 gttggtgacc aatttccacc tgccgcgaac aacgctgctg atgctggtgt ccgcgtttgt
     7021 gggactggag ccgcttcggg ctgcctatga ggaggcggtg gcggaacggt acaggttcta
     7081 cagttacgga gatgcgatgt tcatcgcgag gggactgtct gccgctacat aagctctttg
     7141 tggatcttca cgggaacttt gctcttcatc gattccgaat aggccatcaa cgcccgctgc
     7201 tgtttctgga acagcagatt ctggaaaatc cgttccttcg caatcgccgc tttcgtcgga
     7261 tcagattctc cctcgcgtga gacgagcgtc tgtgcctctg catattcaga aggcgttaac
     7321 gcgacggcat ccatgatgac caggcgggcc ttgttggtga gaatatcctt ggcttccatg
     7381 gcttcgaacg aggcgggggt caagcgattt gctgccagca gtcgccgata ggactcggga
     7441 tcgaagttcc cattcttctg aaactccgtt cgctgcatga tggctttccg caaatcttca
     7501 tcggagaccg agagtcccat ttctttggcc acgtgcagcc acatgcgatt atcgatcagt
     7561 tgttccagca caaactgctt gaggaattca tccttgatgt cgttctggcc tttgtctttg
     7621 tagaagcgat acgtattttc ataggcacgg cgatattcat ccagcggcac gacctggtcc
     7681 ccgaccgacg ccacgacatt gccgctctgg tcaccgaatc cccaccagcc catcgtaatg
     7741 acgaacgcca gggccagcgt gcccatgatg gatttgagaa accacgggta atcgtgtgca
     7801 gcttcgcgta ataatttgat catcgaatgc gcctcttctc ggcagggttt cgcggattct
     7861 actcaggcga cgacagcaaa ggcaagagga agaagtgcgc ggagagcggg tgcgagaaga
     7921 ttgcgacttt gtttgtgcag gtcaaagaga tttgctatag tgtcgaagaa ttggggagat
     7981 ttccggtggc ggaggaggca atcgggcgca gcccggtcga actgggcgtc tatcccaagg
     8041 cccaggtgct gaatcgcttc atcgcgaaaa tgatcgatct cctgatcgtg gcggcggtga
     8101 gcaaactggt ccctcccatc ggtgtcctgg ccggacttgc ctatattttg ttgggcgacg
     8161 ggtttggtgg tggtcgcagt gtcggaaagc ggctgatcgg actccaaacc atcgttccac
     8221 gtacgcggga cccggcggga ttccgggagt cgatcattcg caatctgccc tgtggtcttg
     8281 ctcagttggc atttgagata ccctatgtgg ggtggatcgg atggggcgcg gtactctcgc
     8341 tagaaggatt attggtcatc ggcaatgagc aaggccgccg gttgggcgac gaaattgcca
     8401 agacgcaggt gctggagagc ggacagctgg atgtatcgga ctgaatggct cgatgctgct
     8461 gaacgaagca atcgtgtgaa gggagagttc tagtggcctt catgagtgat atgttcggat
     8521 ggttctccaa tgatctggcg atcgatttgg ggacggcgac cactctcgtg tacgtccagg
     8581 ggaagggcat tgtcctcaac gaaccttccg tggtcgccgt tgagaaaaag acggaacgtg
     8641 tcatggccgt cggcgccgac gcgaagcgca tgttgggacg cacgccggga aatatcatcg
     8701 cggtgcgtcc gatgaaggaa ggcgtcattg cggatttcga aaaagccgaa gcgatgctga
     8761 gccacttcat tcagaaggct cacaatcgca cggcattcgt gcggcctcgc atcatcatcg
     8821 gcgtaccctc gcggattacg caggtggagc agcgggccgt ccgtgattct gccgaactcg
     8881 ccggtgcgcg tgaagtctat ctgatcgaag aaccggtggc agccgccatc ggggccggtt
     8941 tgcccattac cgaaccgtcg ggaaacatgg tggtcgacat cggcggcggc acgaccgaca
     9001 tcgcagtgat ctcattgggc ggcatcgtct atagcgagtc ggtcaaagtg gccggcgaca
     9061 gaatggacga tgccatcatg aactacatta agaagaaata taatctgctc atcggcgagc
     9121 acatggccga acgcatcaag ttcgaaatcg ggtcggccta tcctttcgaa gagcggaaga
     9181 ccatgatgat caagggtcgc gacttgatct ccggcattcc acggacgttg gtggtggatg
     9241 atgccgaagt cagagaagcg ttgcaggaac ccattggaac tatcgttaat gccatcaagg
     9301 tggcgcttga aaacacaccg cctgaattgg ccggagacat catcgatcgc ggtatcgtac
     9361 tgactggcgg cggatccttg ttgaagggaa tggacactcg gtttcgtgag gaaaccaatc
     9421 ttccgatcat tacggtcgat gacccgctga cctccgtggt cttgggcgtc gggaagattc
     9481 tcgatgagtt ggaccttctt cgcaaggttt cggttatgtc gcagtgcagt acctctcgat
     9541 gacctccatc ccgagtgtgg atggctatat cgcgctcaac atacggcacc cggcgtctcg
     9601 ctatcgtctt gttcgcctgc ctgctcgtcg ccctctttct tctccctagt caaagccaga
     9661 ggttgctgca gtacgtcggt ggaccgctcg gtcaagtcct cagtcttcca ctcgccgcat
     9721 tttcctccgt ggatcatggc attgccgaca tgtggaacgg ttatgtcgcc ttgcagacgg
     9781 tgcgtgagga aaatcgccag cttcatcgcg acatcgagtt actcaaagga cagaataatc
     9841 agctgcgcga gtccatgaca gccgtgcaac ggtatgaggc cttgctgaat tttaagcagc
     9901 aatcgccatc gcagaccctg gcggcgcagg tgatcgggcg ggatgccacg aactggtacc
     9961 gcgggatgat cctcaacaag ggtgagaatg acggagtgag aacggaaatg ggggtgatca
    10021 cccctgccgg tgtcgtcggt cgggtggtga aaaccaatgc gacctcgtcg gtggtgctct
    10081 tggtcacgga cccgaacaat gcgattgccg gcgtcgtgca gcgaacccgt gatgaaggta
    10141 tcgtcgaggg aaccagtcat gggcgggcgc gactcaagta catcccgctg ttatcgaacg
    10201 ttcaggccgg tgatcgcgtg gtgacgtcgg gcctgaccgg cgcgttcccc cgggggctgg
    10261 ccatcggaag cctgattcag gtcgaaaagt cagaagggga tttgttccaa tccgcggaaa
    10321 tcgagccgga agtggacctc tccaaactgg atgaggtgtt gatcatcacc gctccctatg
    10381 aggatgccga cgccgcgcag aaattgcttc aggccattca tggagacaga aaaaagccat
    10441 gaagttcctg ttgtacctcc tgctggcgct ggtggtggtt cctttgcaaa cgacattgct
    10501 gcattacgcc agcgtattcg gtgtgcggcc cgatctcggg ctgatagcgg cctgcctggt
    10561 gggatttctc ggcggagagc tggacggttt aattctcggc ctcattctgg gatgttttca
    10621 agatatgctg tcagcaggag atttgtggat caatgtggtg accaagggcg gaagcgggtt
    10681 cctggccggc ctggccggga ggcatatggc ccacatcact ccggctgtca tgacggccgg
    10741 cctcgccatc atctcctgcc tgtcgagcgc ggtctttctc tattcgatga atcccgcagg
    10801 gctggatgaa gtctggttgg gcgtgcggtc caccgtgctg gtacaggccg gacttgacgc
    10861 cgctctcggc gcgggcctgt atctgttgtt ccgtcaacgt tggtctgatg atcgtatggt
    10921 cacggaggga gccctctaaa tgaggaacgg tctctggcaa gagaccggcg cagggcaacg
    10981 atggcgacag taggcttcaa cgactccgat cttctcgatc ttcagcggcg actggtcatt
    11041 ctgcgtgtgg gcttgctgtt ggttgtggcc ttgctcgcct tgcgcctctg gcatctccag
    11101 attcgggaag gcccctatta tcgggatttg tctgagaata accgaacgcg ttccgtggtg
    11161 ctcgagccgg cgcgcggact gattttcgac cggaacggcg tgttgctggc gaacaatgtg
    11221 ccgagttttg ccttgtacgt gacgctggag gacgtgaagg atcgtcaggc gttagttggg
    11281 caattggcct cgcttctggc cctcgacccg gaagtgattc agaaaaaact gtcgaccggc
    11341 aagggcggaa aattgcagcc acgcaaggtc aaagaccggc tgacattgcg cgaggcgacc
    11401 ctgatcgagt ctcaccgctt ggacttgccc ggggtaatga ttcaggtgga atcgcagcgg
    11461 aactatcccg cgggcgctgt ggcggcgcac ttgttggggt atgtcgggga agtctcggcc
    11521 gatcaacttg aaaagccgga tttcgcagat ctgcaccagg gtagcatcgt cgggcagtat
    11581 ggcgtggaga agtggtttga ccggcaggtt cgtggccgcg cggggcaaaa gagtatcgaa
    11641 gtggacgcgc tcggccatga gaaacgtgcc attgtgtcag acaagccgct cgcgggtgac
    11701 gacctctatc tgaccatcga cgccaagctg cagaaggtgg ctgaagacct gttgggtgat
    11761 gaatcaggcg ccatcgtggc tctcgatccg acgaatggcg atattctggc gatggccagt
    11821 cgccctgggt tcgacccgaa cgtattgtcg aaagaattaa cgaacaagca atgggtcgaa
    11881 attgtccaga acgaacggcg gccgctgaac aatcgtgcga cgcagggaca gtaccctccc
    11941 ggatcgacat tcaaggtcgt catggccgcg gccgccctcg aatcgaacac ggtcacgcct
    12001 tcgacgatgg tgcgttgcaa tggcggctat cagttcggca atcgattgtt tcatgactgg
    12061 aaacagggcg ggcatggatc agtcgacatg aatgaagcgt tgatccactc ctgcgatgtg
    12121 tatttctata ccgttgggca gcggatgggc atcgacacga ttgctgaata tgcgaagcaa
    12181 ttcgggctcg gacaggagac aggagtcgag ttgccgtcgg aacgtgtggg catcgtgcct
    12241 tctaccgcgt ggaaacagaa ggcgcgtaat cagccctggt atccgggcga aaccatttcc
    12301 gctgcgatcg ggcagggata cgtcacggtg acgcccttgc agatggcgag tgtgatcggc
    12361 acggtcgcga acgacggtgt gtcgatcaag ccgcgcctgg tacaggccgt gatgaaccgc
    12421 acgacgggag agcgggcaga gtttccgccc acggtgcgcg gaaaagtggc agttaaaccg
    12481 gccaccctcg cgctcatcaa gtcggccttg gccgacgtgg tgaccaaagg aacggcaacg
    12541 cgagcaaagt ctccattggt cacgatcggg ggaaaaaccg gcaccgctca gacggcggcc
    12601 ctccgtaccg ggccggagaa agacattcca aaaaagtttc gtgatcatgc gtggttcgtc
    12661 gcctttgctc ccgtggaagc gccgcgcatt gccgtcgccg tcctcgctga gcacatgggg
    12721 cacggtggat cggctgccgc gcccttagcg aaggacgtta ttgaagccta tgtgaaagcc
    12781 tatccccagg tactgcaggg ggcaccggca agcgggcggg ccaagtcgcc tgcggtgccg
    12841 gttgatacgc ggccgaagac gtgatgattg atcgggtgat cgacagtcgg gggttggata
    12901 gtttcgatct tcgcttcatg gggctgatcg ccgtcatcct gtcggtcggg gtgctgtcga
    12961 tctatagcgt gacgcactct caggacaccg cctttccttt ctatctcaaa cagctggtct
    13021 ggattctgct gggaaccgtc gcgtttctcg tgatgtatct ctccgattat cacaagattg
    13081 cgcgcctggc gtatccgacc tacgcggtca ttttaatcat gctcgcggtc gtgttgttca
    13141 tggggaaatc gagccgcggg gctcagcgat ggattcccat cggaccattc gcgtttcaac
    13201 cgtctgaatt cgcgaaactg gtgctggtgc tggtcctggc caactattac tcccgggcat
    13261 cgcgcgtggg atggctgcat cgggtggtgt tgccgggctt gatagtgttg ccggggctgt
    13321 tgttgatctt gaagcagccg gatttgggta gcggattaag ctttttggcg gtctatgcgg
    13381 ccatgttatt gatggtcggc gtccggtcga aggcgttggg catcattttg ttgctctccg
    13441 ttatgctgtt cccgttcgtc tgggaagtgg tctgggcctc gctgcacgac tatcaacgcg
    13501 aacgggtcat ggccttcgtg gatccggatt acgatccggg aggcaaaggc taccatgcgt
    13561 tgcaatcgag aattgccatt gggtcaggcg aactatcggg caaaggattg tacggcggca
    13621 cacaaagtca gctgaagttc ctgcccgaag ggcatacgga ctttgtattc gccgtctatg
    13681 cggaggaatg gggctttgtc ggcgtcctcg tcctgctggc tctgtttatc gcattgatat
    13741 gggtgtcatt ggaaattgcc gcgcgtgcga aggacacgct cggggcgtta ctcgcggccg
    13801 gtatcgtcgc catgttgtgt ttttgcgtgg tcgtcaatat cggcatgacc gcagggatgt
    13861 ttcccatcgt cgggattccg cttcctctgg tgagttatgg gggcagcgcg accatcatga
    13921 cgatggcgtc attgggtttg ttattgaatg tgaagcgacg tcggctgacc ctgttttact
    13981 aggcgatgcc tggacagcgg ccgacttgtt gtgaaataac catgtgaacc agaggggcgt
    14041 cgggctgccg gcgagacgag agaacccgga catctgtccg cgtgcgtctc ccaatccgat
    14101 ccatctgggg ttaaaggagt gtgtatgggt gtggagatcg caatcagtat cgcgcgggaa
    14161 gaaacccgcg ttgccgtcct ggataatggt gtcgtgacgg atctctatgg cgaccgggcc
    14221 aaggatcagg attacgtcgg caatatttat aagggacggg tggcgaaagt cttgccgggg
    14281 atgcaggcgg ctttcatcga tatcgggctg gagaaggcgg cgtttatgca tgtctccgat
    14341 ctgtctatcg atgtagagcc cggagatacc ctggtcgaag gcgacgacga agacaataag
    14401 gattccgaag ttcccaagcc taagcggcaa agctcaaagc ccatcgaaga gttgctgtct
    14461 gagggacagg aactcatggt gcagatttcc aaaggcccga tcggcacgaa gggatcacgg
    14521 gtcacgacct atgtgtcttt gcccggacga tatctggtgt tcatgccgac cgtggagcat
    14581 atcggtgtat cccgccggat tcctcgcgat gaagaacgcg cgcgtttgaa ggaaattatg
    14641 aaacgggcgc ggaggccggg gttcggctac atcgtgcgga cggtcagtga aggggtgaaa
    14701 gaagaagaat tgcgctccga cgtcgagttc ctgcatgtcc tctggcagga cgtgcttacc
    14761 aagcgtgacc agcagcccgc ccccagcctc ttgcatagcg atttgagcct gagctttcgc
    14821 gtggtccggg atctcttcgg ccgtcgagtc gatcgcctat tgattgattc acgcgaggaa
    14881 tacaacgcga tcaaggggtt cgtgcaacgg ttttctccgg agcagacgtc ccgcatccac
    14941 ttctatgaca aggaagaaag cctgttcgat tacctggggg tggagcagga aatcgcccgc
    15001 gcgatgagtc gaaaagtgtg gctcaagtcc ggggggtatc tggtgattga tcacaccgaa
    15061 gccatgacgg tgatcgatgt gaataccggc cggtttgtcg gcaaacggga tcaggaagaa
    15121 accattctcc ggaacaactt ggaagcggca cgggagattg cctaccaagt gaagttgcgg
    15181 ggcattggcg ggatcatcat tgtcgatttc atcgatatgg agcgggaaaa gaaccgcgac
    15241 aaggtctatc acgccttggt ggatgccatg tcttcggaca aggcccgaac cagaatttcc
    15301 cgcgtgtctg atcttggatt gatcgaaatt tcccgtgagc gggtgcgcga ggatctgctg
    15361 cggtcactct ctgaaccgtg ccactactgc gacgggcgcg ggtatacgaa atctcccatg
    15421 tcggtggcct atgaaatttt cagggaagtg cggcggctcg gtcacgagat cgagcagcaa
    15481 cgtgtcgtcg tcggggcgca tcctgctgtc gccctgctgt tacaggagca ggaacagccc
    15541 ggggtagagg aactcgagcg gcggtatagc gccaagattc ttgtgactcc ggatgatcgt
    15601 ctacatctcg aacagttcga cttagtcgtc atgtaataca gcaaggcgcc gccctggctg
    15661 gttttacgcg aacgtctcgt ctctgacagc gcttccagca ccacggtcac tgagtcgcac
    15721 cgaccatgcc tgatgaactc agacgatggt gaatagtcta tctctccgcc catggctgat
    15781 gctccgtgcg atccctggtg tgggcgatgc cattctgctc aagctggtgc aggcgatggg
    15841 ctctccggac gcggtgctgt cggcaacgca agcgagcctg gaagacgtcg ggtgcagagt
    15901 tccgttgatc gacgccattc gccgtgggcc tgacgcacag gcggtcaaac agcttgatcg
    15961 ggaactggcg gcggtgcagc atcgtcagat caccgtgctg acgtatctcg atccgcagta
    16021 tcctgcgcct ctcaagacaa ttccggaccc tccgccgctg ctgtatgtcc agggaaccct
    16081 gctggaaagt gatcggcaag ccgtggcgat agtcgggacc aggaaagtca gttccgccgg
    16141 ccgggtgcta gccgaggaat tggcctgtga gttggcggag atgggcttca cgattgtcag
    16201 tgggttggct cgcggagttg atgctgccgc acatcgcgga gcactcgccg gcaagggacg
    16261 gacgcttgcc gtgatgggat gtggattgga tcgcacgtac ccggccgatc atgccgcgct
    16321 ccgccaggaa atcgagcggc acggggcggt cctctccgaa ctgccgctcg ggtccgcgcc
    16381 gcacagctac cacttcccac gccggaaccg catcatcagc gggctgtcgt taggcgtcgt
    16441 ggtgaccgag gccgcggttg aaagcggctc tctcattacc gcgcgattgg ccggtgaaca
    16501 ggggcgcgag gtgtttgcgg tgcctggctt tgtgaaggcc gagaatagtc gcggaccgaa
    16561 tagcctgctc aaggatggcg cgaggctcgt cgaatccgcg caggatattc tggatgaatt
    16621 gttgcctcag ctggatgccg cgtttcgcga acggctcggc gatcgcgcga gaggagcccg
    16681 accggctatg caattagggc ccgaggaagc gatggtatat gatgccgtat cggtattgcc
    16741 gcaatcggtc gatgatgtca ttcgacgcag cgggcttgcg gccgcgcaag tcaccgcagt
    16801 gttgctgtcc ttggaattga aaaattgtgt ccgccagttg cccggcaacg agtatgtgcg
    16861 cctctgagtg gacccgttga gagattcaag ctgacagggt ctcgatattt gttatagtga
    16921 gtgtgatagt ctgggcgcag gtacttagga acatggagcc acatggccaa atctctgatc
    16981 atcgttgagt cgcccaccaa ggcgcgaacc atcaccaagt atctcggacg aggatattcc
    17041 gtcatggcct ccgtggggca tgtcaaagat ttgccgacca gtaaactggg cgtcgatctg
    17101 gagcatgact ttgaacctca gtacgttacg atcaagggca aatccaaagt ccttgcagac
    17161 atcaagaaaa aagctcagca agtggatacg gtcttcttag cgccggaccc tgatcgtgaa
    17221 ggggaggcga ttgcctggca tatcgcgcag gaactgcacg gcaagggcaa gaagaaagac
    17281 ggcaaggtct accgtgtgct ctttaacgaa attacagaat cggcgatcaa acgcgcgctg
    17341 aagtcccccg gtgaaattga tatgaagctg gttcaggcgc agcaggcgcg ccgggtgctt
    17401 gatcgtatcg tagggtacca gggcagtcag ttgttatgga agaaagtgcg gcgcggtctc
    17461 agcatgggac gcgttcaatc cgtcgcggtg cggcttattt gcgaccggga acaggaacgc
    17521 gaaaagttca gagccgagga atattggtcc attgtcgcgc tcttgcgtgg agacaatcct
    17581 ccggctttcg aggccaagct gcattccatc aacgggcagg atgccgatat cggtacgagt
    17641 gcgcaggccg agcaaatcct caatgccgtg caaggcaaag catttgtcgt tcaatccatt
    17701 gagcggaaag aaaaaaagcg aaacccggtc gcgcccttca tcaccagccg attgcagcag
    17761 gaagccgcca ggaaattgca tttcaccccg aagaagacca tgacgctggc gcagcaattg
    17821 tatgagggcg tggaaatcgg cgcagaaggc ccgatgggtc tcattacgta tatgagaacc
    17881 gattcgccga gaatctccca agaggcgacc gaagatgcga ggctggtgat tcgcgagcgt
    17941 ttcggagagg agtatctgcc ggcgactcct aatgtgtaca agacccaaaa ggcggcccag
    18001 gaagcgcacg aagccattcg tccgacgatg gcggctcgcg atcctgaatc catcaggcaa
    18061 tttctagagc aggatcaata caatctgtac aagctgatct ggaatcggtt cattgcctca
    18121 cagatggtcc cggctatctt ggatgtgacg cgggtcgatt ccagtctgat cggaacacca
    18181 gaacgctacg tgtttcgcac gaccggcacc gtcgtgaaat tccccggcca cacagccgtc
    18241 tacctggagg ggaccgacaa ggaattaccc tcgcagaagc ctaaaagtga gcaagagtcg
    18301 gaggacgagt cggaccggca gcttcccgta ctccgtgaag gagagtcgct gcatctggtc
    18361 actcaggaag cacagaccct gccgggcctt atctcaaagc agcatttcac ccagccgccg
    18421 cctcgttaca acgaagcgct gttgatccgt gaactggaag aaaagggcat cggtcgccca
    18481 tcgacctatg ccgccatcat ttctacgatt caggatcgga aatatgtcga gaaggtcgag
    18541 ggccgtttcc tgccgaccga aacgggtcgg acggtcaatg actttttgct gaaggggttt
    18601 ccggatctgc tggacacaga attcacctcg catatggaag agcaactcga cgaagttgaa
    18661 gagggaacca agccgtgggt gtctgcggtg agggagtttt atgccccctt tgtcagggaa
    18721 atggagctgg cgcaaagtat tcccggcccc aaagacatcg ttgagccccc gaccaacctg
    18781 ccttgtgaaa agtgcggccg catgttggag atcaagtggg ggcgaaacgg aaagtttttg
    18841 gcctgtcctg gatataagga agatcctccc tgcaagaata cgcagaactt tgaaaaactt
    18901 cccgacggga caatcaaaat cgttcccaag ctggaggaga cgaccgacga aaagtgtgag
    18961 aaatgttcaa gccccatggt cgtcaaatct ggccgcttcg gaaaatttct agcctgttcc
    19021 gcttatcccg aatgcaaaac gacgaaagcc attgccttgg gggtgaaatg cccacagcct
    19081 ggatgcggtg gggatctggt tcaaaagcgc acgaaaaaag ggcgaaactt ttattcgtgt
    19141 agccgttatc cggagtgcga gtttgcgctt tgggatcggc cgattaataa gccctgtccc
    19201 aagtgtcagg ccccgttcct cattgagaaa gtcagtaagc aagccggccg ctctgtgcag
    19261 tgccgtaatg aagaagagtg cggctaccgc gaggccggat aatctcgacg cacggtctgc
    19321 ccgtcccaag gttgtttctc accttccttg ttcttcaatc ccgatcgacc gaatctccat
    19381 ctgcactgta ccgatctgct cgcgcgagga cacgtgttct gatgaggaca atgagaagcg
    19441 ttctcggcga tgttgagaac cgccctcata aatacgtata tcaatggttg acaagccctt
    19501 cgagaatctc taagtaagga gtacaataat ggggaagcag gaattgcgta gcgactaaac
    19561 cagatactag tccataggcc gtgagacgtt atggccggag gagggggata tgaaggcaaa
    19621 gcaaggcgtt attgagctgc tgaataaagt gttgacggcg gatcttaccg ccatcaatca
    19681 atatttcgtc catgcaaaga tgtgcgccaa ttggggctat gaacggctcc atcacaaagt
    19741 cagggagcgc agtattgatg agatgaagga tgcggacgaa ctgatcagcc acattttgta
    19801 tttggaaggt gtgcccaacg ttcaacgcat gaacacggtc catattggag agaccgttcc
    19861 cgagcagttg aagctcgacc tgaaggccga gcaggaaatg ctcgccctat tgagtgaagg
    19921 agtagtgcat tgtacgaagg tcgcggattt tacgacgcgg cacatgttcg aagatatggc
    19981 gaaggatgtg gatgaacata tcgattggat tgagactcag atggaaacga tcaagcagat
    20041 aggcctcgag aattacctcg ctgagcagat caagaaagac agttaaaccc gcgccggaat
    20101 ggtgcagtga ggacatgggc atgaaagcga aagagggcgt tctcgatatt ttgaacaagg
    20161 tgttggtaag tgagctcacg gcggtgcacc agtatctgct gcacgccgct ctgtgcaagc
    20221 attggggcta tgctcgactc cacgagcact ttagccattt ggctcaggag gaggtcagcc
    20281 attcctccgg tttgatcgat catatcctct accttggcgg atccccggag atgggacgtc
    20341 tggatgccgt ggcagcgggc aagaaagtgt cggagctctt ctcggcagat ttagcttttg
    20401 agcgtgagga cgcagatgcg cttcgtcaag gcatcgcaca ctgcgcgaaa gtcggggatt
    20461 tcaccacccg acatcggctg gaagacatga tcatcgatac ggaagaacac atcgattggt
    20521 tcgagaccca gcttcgaacg atcgaacagg ttggagaaga tcgctacctg gcggaacaac
    20581 tcaccgccga caagtcctaa ccgcaaccgg aagtgatggc gggtcggcga gctcaaacac
    20641 tggctgaccc gccattcttt tattctcacc ctgcattccc agaccgcatc acttgttgag
    20701 ctccgcctca agaagtctgc tgcaagccct gaatcgtttg tctctgctgt cgagcaatag
    20761 tcaaacgaat agccatggga gactgtcccg ttgctcgctt gaagaagtgg ctgaaggcga
    20821 attgatcgct gaaccccagc gctgaagcga tttccgtcag cgtctgtctg gtcgtgatga
    20881 ggagcgcacg ggctcgttcg actcgctgtc gccggagata tttagagaac ggctcgccca
    20941 tgatgcggca aaacagatca gagcaatatt tttcagaata gccgaggaaa ttcgccaaga
    21001 ccttcagcgt cacgccacga tggagattct cgtttacaaa ggccgcgatg cgcgaaggtt
    21061 gcgtgtgagg gataggcgga ggctgtgttt ccgggttgat ggcatgggag aggttaaaaa
    21121 gatcacatat caggtccccc aagtcctgtt ccgttctgac ctgttccagc cggtccgccc
    21181 actgtggcga ttgaactcga agttctgatg agaaagagtg caaccgatca aatgaaatat
    21241 tcgaggtaga cgattttcct gacatcatgc cctccatcga gagttacacc gcccacggta
    21301 acgagtaatt tggagatacg actggttaga tgcctgagcc tgtcatgtat gcttctggca
    21361 catgttctgg tgacgattgc gaatgcatgg cgtcgctgga gagatcggag ctctggtatg
    21421 cccggtagtg atcgtgattg cgtagaagct caatgaacgt gacgcagaac gccggatcaa
    21481 attgagagcc ggcggaggca tgaagcgttc gcagggcgct gtcccacgag ttggttcggc
    21541 tggtgatgct gtcaaacaca tcggcaatcg cgagaattct cgctgaggcg ggaatgtatt
    21601 cgccgcgcag cccgtaggga tatccggctc cgtcccaccg ctcatgatga tgggcaatca
    21661 gccgtgccgc cacttgaaga aatgtatagg gagtcagcag tgcggcccct tcgcgaggat
    21721 ggctttggat cagggcatag tcgttaagtg tatgaggagc tggcgcaccg agcagatgtc
    21781 ccggcatcat gaggagccca acgtcatgca gcaatgccgc atagtgcaaa tccaggcgtt
    21841 gatgtttggt aagcctaagg cgatcggcca gcaataaggc ataatgtgcg gtgcgttccc
    21901 catgtccgaa gtgcccggga atgacggttt cgatccgtcg tgccaaacgc tcaagttgga
    21961 tcaccctgac cgcctcaagc gactccgggg aaagctgtaa ggtattgagg gctgagagct
    22021 gcgtcgccaa gtcttgaata agttgacttg tggttggtat cggtcgtgtc gaacgatgca
    22081 tgaaagtcct ccaaaatgtc agagcccaag accatgtgaa tggtcttggg ctctgggcga
    22141 aatacctctg gccgcacatg agtggtgtac atgccgcaat gctaggtcat gcgtgttctc
    22201 cgcgtttgcc tgccattaac ggttgtttca agatccacat ctcgtggaga ggaatgacga
    22261 acatgatgaa gaatcctgcc gtcatgaatg tatcgcctgt cagcatggtt aagacgaaga
    22321 cgggagaaac gtaactaatc agccaaggcg ataagaggtc gagtatgact cctccgaagg
    22381 aaagccatgt cgtgactatt tttagtcggt tactggcatt ggtcaaatag aggacatgga
    22441 caaggatcat aaacacgacc ggcatcgtga acagatggaa atgggtaatt tcagccagct
    22501 ctcggaaaga catcggttcg ccgaaggtgg cattggagcc gcggtaatga tcagcaatcc
    22561 cttggggcga taagccagtc atgctgtgtg cccagaaaaa agagaacgtg aagcctgcaa
    22621 gcatcaacag gagaaagaat gtgtagacga gcctgatatg gcgatccgaa tcgcggaggc
    22681 gaaaacgggt attgaaatta cgcatgtctg ctgaaaatca gttcccaaaa atcgacttga
    22741 agaagccctt ctccgttttt tgagcggtta cagtgtcgct gccccgccct tggggtttca
    22801 agtaaaattc gtccacgagg acgagaacgc gtttgatccc tgccgtcatc gagcgaacgg
    22861 acatggtggc gccgctgatg ttgatgatat ctttgttaat ccgcactggg tcgagaacgg
    22921 attttccttc atattgcaca ttaaatcgct tggtccgtac ctcgctgccc cgcgcctcgc
    22981 gaaaggtgag caattccaca ttggaaacca ggccatgagc atctactccg accatatagg
    23041 tcatgggctt gtgtttgccg atggtgtttt ggaccagggc gtacccgtca atgtgtgtgc
    23101 cggtctcgcc aatatagact tcgaatgatt cctccgggaa tttccacccg atacggcttt
    23161 cgatcaatgc tttcttgtcc tggggaaggt tcagcagttc ccttctcacg cgctccgatt
    23221 tcggaaacat gagtttgacg gcttcttctt cagtaagaaa gacttcggcc agggacattt
    23281 cctgctctgt caaataacgt ttgagctcat tgtcccatac acgctcggcc tgggcgttga
    23341 gtggagcgag agcgaaggcg ccggtgagag tgagcagcag caataacggc atcataacgg
    23401 cgtacacccc tgttgtttta atttttcgtt cagtcgagtg gtcacctctg ctgtcgcctc
    23461 ttcggatggt tccacgggcc accacttagt ggcctgggac gtgacccctc ctcgtgaatg
    23521 ccagcgctgg cgtgtttcca gcagactgac ggagacagcg ccattcgatg tcttgagggc
    23581 gagactcata cgcgcgcgct ctctgttccc aaatcctgtg cgcccgaaaa tgccataact
    23641 gcgctctttt ccatccatct ccagccagcc ggtctcgatg acgcctttgg ctttgtcctg
    23701 ggacgtgatc gagtagcttt tcatggtatc catcgcggcg tcccagacga cgtcttgtga
    23761 gcaggaataa gactgctcgt gactgccgct gagcgaggcg caaccgatgg taaggaatgc
    23821 tgctgctgcg ataaatatgt gtgccctttg cacggaaatc ctgcctcttg tgaatccggc
    23881 ggccctcccc atagagagag ccgccggggt gtgaatttag aaatacgtcg ctgcagaaat
    23941 cacgaatgcg ctgtcatgaa tgcgctgtcc agtattggga ttaaacgatt tcggcatgta
    24001 ttggtagctg gccttgaata cggcatcttc gatcggccga taattgagac cgaacgaaaa
    24061 ctgctctaat tcgccagagt tttctccacg attgtccata ttgagattga cccggtcata
    24121 tctgaacacg gcggtgaatg ttgatccctc accaaagcgc ttcggtgata aggccgtgag
    24181 gaacgcgggc atgaaatgat agttcgcctg gacatagaat ccctgcatcc gctgcggagg
    24241 ggcggcaaat gagttcaagg tattaagtcc gctaagtagc gagccgggag tggcgaaggt
    24301 ggctccctga accgcgcggg aattgccccg tgcataggcc caggccgctt ctcccaagag
    24361 ctcgaatggg ccacgttgga aggtccaatc gatggcggta atgctgaggg ggttgtagct
    24421 caggggggaa gcatttccga agtagctgga tccggcaact tcgacaccga gcatcgggct
    24481 gaacgcgacg cggccagaga ccgcctttcc gttgttgata tccaacccat cttcagaggg
    24541 gcacttgcgc tgtcgggaac gtcgagtgcc gtcttcttca cgaatgcgag ggcttccgtc
    24601 cccgttatat ccgcatgggc cggtggtgac atagaattcg tagtcaagct tgctggttcg
    24661 acccgggtac agggtgccat aaaagccggc gcccgtttcg gccatcgtcg acggaatcac
    24721 cagacgactg acgagtggtc gatcggtgag atcgttgagc ggcgagtcat gcaacaggtt
    24781 gaacttcccg atcggaacca aaagaatgcc tgcgcgaagg ttgaacggtt ctttgaccaa
    24841 gtagtcgatg tgggcgaact ccaagccgat ttcggccccc tgcgtggcat cttcacgaat
    24901 gccatgctcg atttcaattt cagcggcgaa tttcacatgc tcggtgatgt cggcatagat
    24961 gaatggaaca aatcgttgtt gatcaaatcc gttggtggtc ccgcttccca tgcctgattc
    25021 aagaacgcca ttgcggtgtg aacggtactg tatgtccatg tacccaccga cgatcgcttt
    25081 tggcgcggcg acaaacggct tggcataaac caagcgtcca gatccggtgg acccgaacga
    25141 caatgctgct tggttctcgg ttcgacgttc ttttccgact tccggaaggg agccgatacc
    25201 cggctcatta ccaggggcac ccacagtttc cctgtgatcg ccggcacctt cgcgacgttc
    25261 atgctcctgc aatcgctttt caaccgcttc gtcaaccagc ttcttgatct cctccggggt
    25321 catgttatct gccaacgccg gcattgccgg taaactggcc aggaccagag ccccgaggat
    25381 tgcccgaatc ttcatgggga tcctccatcc gcgagtaagg ttatggtatg actcgggact
    25441 caatggtctc tggtctgtaa tgaaagcctg agtgtggggt tgaagccctg gatattgtcc
    25501 ggtgatgagg cctcctttcg tgaatcgact catgctggca ccgtacccca tccttcgatt
    25561 gaggtaaaag gaatgaccac aatgcgtttg cagcgcttgc atttaagctc gagcccttgt
    25621 ccgcgaactt tggcgatcag ttgaccgcat tcgcagcgtg tttcgctgtc ttggcacaag
    25681 gaatgtattg cgttattggt tggtgtcata gtgaagtgtc gagttcctgg tattgagaac
    25741 gattattaat atcccgatca gtagggtaat gtcaagacgc ttttgggaag gtgtgctgag
    25801 ctcatcggat cctgaggaaa cggacgactt agcgggagat tagggattgg gcaggcacat
    25861 gggaggcggc atggtgaagc gggaattcac gacaatgcca aggttgcaat tttcctcagt
    25921 ggcagagaag atgactcgct gcagattccc actaaccaag gaccatgccg atgaagttgt
    25981 ccattaatag tcaccccttg cgagcatttc tattcatgtt cgtaaacgat gctgtcctac
    26041 ggcgcgggtt tgctttggca attcggggaa gcctggggct gctattgtta cttgggctgg
    26101 ggtgcgcagg tggaaacctt ggaagctctc ccattctcgt caagcgcacc caaatgcata
    26161 tgggtacgtg ggtgagcatt acggcagtcg ccccagaccg agaggcagct caatacgccg
    26221 catcggcagg atttcaggaa atacatcggt tggaagaatt gttgagtact tggattaaag
    26281 atagcgaact gtcacgggtg aatggagcag cgggtaaaga agctgtctct gtcagtcctg
    26341 atacgatgaa ggtccttcag gcatcagttg cgatggctcg gcttaccggc ggtggcttca
    26401 acattcttgt cggccccgcg gtcgaggctt ggagtgtgtt ggatcgccag cagatcccgt
    26461 cggagtctga gctagagaac atccgtcctc tcacgaactt gaacgcgttg caattaagcc
    26521 aggcgcaggg aaccgtctat cttgcaaaac cgggaatgcg cgtggatgtg ggaggcatcg
    26581 gtaaagggtt tgcggccgac atggcagtgg aggcgatgca aaaagccggt gcaacggcgg
    26641 gggttgtggc attgtcgggg gatatccgga catttggcca aatgcctgac ggaacgacct
    26701 ttccattcgg tatccgccat ccacgtcgcg aaggaacagt actggcattc gtagatcttc
    26761 agaatgaggc aatttctacg gcgggcgatt acgagcgcta tttcgagcgg gatggagtcc
    26821 gatatcatca tgtccttgat ccggtgacct tgcagccggc acgcgactgt caaagtgtca
    26881 cgattgtcgc caaagacggc ctgacggcag atgggttgga tacgggcatt tttgtgatgg
    26941 gcccagaacg gggactcgcg ctagtggaat cgttgcctgg tgtcggcgca gtgatcgtcg
    27001 atcgtgacgg aaaggtctgg gtgtcctcat ccctcaaagg acgagttcga atcaaggacg
    27061 gcgtcgacta gtgttgtcat accttcagcg tgagtcccag tcggagaggc gagcgggaag
    27121 catgctgaac gaagggccgg catcagccag gttatggtcc tagctggtag cggatgggga
    27181 catggagagt gaccggtgat tgttcgagag gctgcgtcag cgcgatcggt gaaatctttc
    27241 gcactgtttc aagtgcggct tgatcaagag tgtcatgccc ggaacttttt gcgaccgcgg
    27301 cggacacgat ttggccatct tcctgaatca caattcggac gaccactctc ccttccacgt
    27361 gcttgagccg tgcatccacg gggtactctt tgagcgcttc aatgcgtggc aagagaatgc
    27421 ctgcaagcca gccataatcg acctttctgg gcgccgggac gttgttcgct gatggggtga
    27481 gcgcggcggt tgaggtcggg gctgggggct gttcggtgga ttgctgtgca gctggaaaag
    27541 gagacgttgg tggatctggg acttcatgat ctttggctgg ggaagtgact ggtgcttgct
    27601 gtggatgaac ttccgccggg gaagggacca ctggcgcggg agaatggtcc gttgccggct
    27661 ccggtcgggg agcttccact gcctcaggaa gaggccgcgc gagagagcgt tgaggtgccg
    27721 gctcgatatt ctgaggcggc gatggaacaa cgggggtagg tgtcagcggt tcatggatac
    27781 tagtgtgaga ggccgggacc gatttcgagg aatgttgtac atcggaggcc gcggggcgcg
    27841 gagagggtgt cggcgtgctc tgcgctgtca tgcgcgcagg agttgaggga acttgcggtt
    27901 tgtcgagcgt gggagttgac gcggatgggg tgacgacggt cacgtcccac tgaaacgacg
    27961 aggatggcgg ggggagtcga atcttggcgg cgaacagggt ggccaagaag accgctgcgc
    28021 cgtgcaagca gagcgagatg atccatccgc ttgcccgcaa gcggacgaaa gcgtccggca
    28081 tgccggcagg gggagcggtc acaagcgcac gacctccagg ctgacagatt ggaagcccag
    28141 gccacggatc tcatccacaa ccgaaacaaa acgttccagc agcgtgacct tgtccgctcg
    28201 cacgacgacc agagattcgc gcgggtgggt caacatcacg cttttgaggc catccgctgg
    28261 aacgggccgg tcgttcaaaa acagatctcc gttggcggtc aaggtgacga cgacggggac
    28321 atctttatga tcccccgcct cttttgcctt ggcgaggttg acaggaattt ggcccgtgct
    28381 gatgaaggtg gcggtcgtca ggacgatgac gagcagcacc aacatcacat ccaccagcgg
    28441 aatgacgttg atttgattaa cctctcgttc catgttgcac cttataggtc gtcagcagtt
    28501 cagcgacacg tcggcgaaga atattgttca tgacaacgca gggaattgcc acaagcagac
    28561 cgaccgctgt tgcctttagg gccaggctca gaccgatcat gatggtattc acggccatgg
    28621 ttccggaggt ccccatcgta tgaaaggtca tcatgattcc taaaaccgtg cccagcaatc
    28681 cgatatacgg tgcattggct gcgacggtgc cgatgatgac gagtcgcttg gtcaacgcca
    28741 tttcaaaggt ctgcacgtcg gtaaactgcg ccagattgac gcgtcgataa tatagccacc
    28801 gctccactgc gaccgcgact gaccacacgc tcagcgcgat caacaggcca atgatcccat
    28861 actccaccgc atttttcagc acgtccattc gttgttcctc cactgttatc gccggggggc
    28921 cgcataacat cgttgcggat gtccttctcc gtcttgttcg gtctcgtgtc aggaagtgag
    28981 tctgatgtca cgccaccggc ggagagccgg gcggttgcgg aaacaagacc gtatgggtcg
    29041 ccaccacgcg cagggcgatg gcggttccaa cctgatagat gctcagcgac gattcactac
    29101 tatgtacaat ttggcctgat gggagttgga tcgtgtagac attctccgaa cctttgaatt
    29161 gccgtgcaag gatacgggca tcggctcctt tagttgggac gatgtggata tcgtcggggc
    29221 gaatcatcac aacaacatgc gtgcccgtcg cgaggtgttg agtattcgga aagtcgccga
    29281 gttctgtcgt caccaagtgg tgcgtcacga ctccggcgat aaaatccgcc tggccgacaa
    29341 aatcggcaac gaagggggtg gtcgggaggt gataaatggc ttcgggcgta tcgaattgtt
    29401 ccaggcgccc cttatttaag accgccacac ggtcagccat ggaaaatgcc tcatcgtgat
    29461 catgcgtgac gagaatggcc gtggtcttgg tttgtctcag cagggcgtga agatcctggc
    29521 gcatgcgact cgccatatcg ggatcgagat tgctgaatgg ctcgtcgaga aggagcagca
    29581 ccggacgaag ggcaagggcc cgggcgaggg cgacgcgttg ctgttgaccg ccggacagtt
    29641 catgcggata ccggtgctcc aatccacgca gcccggttaa cgtgagtagg tcgtcgacga
    29701 ttgcctgctg ctcggttcgt ggaaggtgtc gcaggccgaa tgcaatgttc ttttcgaccc
    29761 gtaaatgggg aaacagggcg tattcctgga acaccatgcc gatatgccgc tgctccgtcg
    29821 gaatcatggt atccggcgag gagacccgtt gaccggacag cgcaatagta cccctggtca
    29881 cccgctcaaa tccggcgatg gcgcgaagaa tagtcgtttt gccgcagccg gaaggcccga
    29941 ggaggcagag aatctcccct tggtggaccg tgaaggtgat gccctcgatg gccggtcgtg
    30001 tgggctcata ggcgcacgat acgtcgcgca attcaaggac ggccgatcgt tcgacggtcg
    30061 tgtccagagt attcgggatt attccctcgg atgcgggctg ttgccggcct gttatagatt
    30121 cgttcacgag gctcacgtga cgcccttctg cgcctctcca cgccagtctt tggaaaccag
    30181 cagcatcagc gctggaaggc tcaagatgac gatgagcaat gcggcaggcg ccgccagttg
    30241 gtaatactct tcgctggctt ccagccacac gcggatggcc agtgtatcaa atcctacagg
    30301 tcgcaacagc aatgtggccg gcagttcttt catggtctga agaaacatca acacccatgc
    30361 ggccacaaat ccattgcgga cgagcggaag cgtgactcgc cacaggcttt ggtgagtcgt
    30421 gcatcccagg gtgcgggcga cttcctcgac attgggtgtc acttgttgca gggccggttc
    30481 catggattgg aggccaaccg gaaggaaatg cagaatatag gcgatgatga gcacgaccac
    30541 agtcccatac aatgccggtg cgaagtgggt aaaaagtacg agaactgcca gtgcggctac
    30601 cggtcccggc agcgcatagc cggcataggc ggcttgaagg caggcaatgt tcagtcgtga
    30661 ggggcggcgg ctcgcgaggt aggctaacgg aaggccgatg atgaccgcgc cgcaggctgc
    30721 cagtgctgcc aggaaactgc tgttccaaat aaagcccaga aagcgcgtgt ccaacgcgcc
    30781 ttgagaaatg gcttccacgc tccattggat caacatggct gccggaatgc caaacgcggc
    30841 tccgaagacc aggacaacgt agctggtgat gagcgctgtt ccgagtggcc ctgcatgttg
    30901 acgtgtcgat cgtcggtatc gacccgatgt ctgatagaag cgactgcgtt ggcgaaacca
    30961 tcgttcggtc accaggaaga tgatggccat cacgacgagc agtaggctca aaatactggc
    31021 ggccgtgtgg tcatatcggc tggtcatctg ttggtagacc gcataggtca gggtttggta
    31081 ccggagtaag gacaccgcac cgaagtctga gatcacatac aggatgacga gtgccaaccc
    31141 cgctgcaatg gccgggcgaa taagcgggag cgtgacccgc cgaagcgtag cccatggaga
    31201 gacgccgcag gcgcgcgcga cttcttcaaa cgagacattg agattttgga ttgcgctgcg
    31261 gaccagcagg tacacgaagg gaaaggtgtc gagggccatg atcatggtga ccccgggaaa
    31321 actttggggc gagggcaggc gagcctcatg gccgaagagc agttgccacc actgttctgt
    31381 cgggccgccc attcccagta ggtgggcata gacgtacgcc agcacgtagg tgggcatagc
    31441 gagaggcagc gcaagggccc attcccatat gcggcgaccg gggaagtcgt atcgaacggt
    31501 gatccaggcc agcgatacgc ctaacaccaa cgtaatcagc gcgacgccaa gggctaacga
    31561 cagcgtgttc cctaagagtt ctggaatgcg cgtggaccag aggcgagccc atacggccgg
    31621 tgcggccgtc atcgcgacat agacaatgta cgcagtgggc aggaccagga acgctgcagt
    31681 gaagagactg atccactgca gggaagatgg cgcccgaact ctggtcgcag taagcatgct
    31741 cggccacagg ggtcagcgaa ggccgacctg ctcgatcagg gtgagagtgg attcgcgcaa
    31801 ttcagcgagt cgggctagag gaacgagtgc cgcgcggaag ctatgccggt cgacgagagc
    31861 cgggtcggct tttacgtccg ggtgcagtgg atattcttta tccaagtccg caaagagttt
    31921 ttgccccgcc tgggccacca gaaactcgat taatagtttg gcgctgtcga cgtgtttcga
    31981 ggctcgggtc actccgatgc cggtcacatt catgatggcc cccatgccgc cttcctgctg
    32041 atcggtcatg atcgcggcga tgggcgcggt cggctgtgct gccagatgac gataaatata
    32101 gtagtgattg acgatgccgg ctgcgacctg gccctttgcg actgcttcca caatttgtga
    32161 acttttctgg tagacctgcg accccgcatt ggtcttcagg ccttggagaa attgtttggt
    32221 gcgttcgtcc ccgaaggtgg tcttaatgac ggacacgccg gcttgaaggt actcgctgcc
    32281 ggaattcggg atggcaattt tgtctttcca ttgtggctgg gccaggtcga gcagcgactt
    32341 gatttgctcg ggtttcacca gagtcgtgtt atagacgacc acccaaaatc ggccggaaag
    32401 cccgatccag ctgttgtccg aagcgcggaa ttgagaggga atggcccgct cgacttcacg
    32461 catgttcatg ggacgaagaa gtttgagttc tcgcgcatgt tcgaggcttc cggcatcgtt
    32521 ggtcagaaag acatcggcag gggtgtggtc cccctctgcc tgcaaccggt tgaccaactc
    32581 ggtggttcct gaggacagga gttcgatctg aatgccgctt ttggtttgga attcgtcaag
    32641 aaccggcttg atcaatcgct cagcacgacc agagtaaacg acgagtttgt cggctgcatc
    32701 cgcggtcttg gcaggccact cctggagtat caacgccgag atgagcaggg tcagagcaaa
    32761 tacggtgcga gggaatgaag ccatgcggcg tgcctccacg agtgaggacg ctcggggcgt
    32821 cctagttgaa attgataagc cgtctcaaga tgataccgga tgtgaagaga atccgtcaat
    32881 ggcggcagcg cgcgcagagg ccgtacagtt ctagtcgatg ggtttgaatg acaaacccat
    32941 tctttgtggc gacttcctct tggagtcgtt cgatttcgca attctcaaac tccacaatgg
    33001 tgccgcaacc ggtgcagatc agatgatcgt gatgaccttt gtgagagatg ttgtcgtatt
    33061 gtgtctgggt accgaagtgt cgagcctggg caatgccagc ctcgcaaaac aggttcagtg
    33121 tgcgatagat ggtggcgagg ccaagatgcg ggtccttctt cgcgagctga tggtacatgg
    33181 tttcagccgt gacgtgttcc tgtcgtagaa acgcggtcag gatcagttcg cgttggcgag
    33241 tcagctttaa ttggtgcttg gccagatgct ggcgaagcac atccatctct ttaagatttt
    33301 tgctcatggt gtgtgactcg gcgtgaaaga ggctcattaa aaacgaaagt gcctcgatcg
    33361 gtcaagaggg gacgcgcagt aggccattgt cgattgacga atcggaagcg gcctgtttat
    33421 agtcccccgt caccgccaat tgtgaggaga ttcgaccctt gcgcaaatcc cacgaaatga
    33481 ccgttgatcg ggccctgctg ctctatgtgc tttcgctggc tgagccctat gggttgttga
    33541 gcgacgtgaa gcttcagcag ctctgtttcc tttgtgagct tcagatgttc ggaaaggggc
    33601 tcaagggttt tcactttgag ttcgtccggt tcgcgtatgg cgcgttcagt cgggatttgg
    33661 acaatgacct gacctcgctc cggcgcaagg aacgggtcga aaacttcagt ccctcagatc
    33721 aggttcgtga tgagacgctc ccgctcctgc tgaaagggat tgacggaaat gaaacgaatg
    33781 aaaaggtgcg cgacctcatg caagcggtca ttgccaccta cgggccgcag gatgtcactg
    33841 ctattatgca gtcagtcgaa tcggtggaat tgagtactcc acagcagccc gatttcaaaa
    33901 ttcccatccg cgatatcgtc tttcatacca cactcctggt gccgtcccga attgaagcgc
    33961 cagcagaatt tctcttgccc gccaatcatc tggcgaagct caatgtctcg atgggatact
    34021 gagctcgatt tcttgagccc acacctgctt gatagttgga attgctcaca aggctggcag
    34081 caggcctaga gatctctctt aacaaggagg atattcccgc tcatcttcca ttaccagcgt
    34141 tgctggtaca tggttttagt ggcgccgggg taaggtgaga gccgactgta tcgtatgcag
    34201 catggctccc gtttcgatgg gttccagcaa acaatactcg atttgtaact cccggaccac
    34261 ccgctgaaac ttctccccct ccagggcagg ttcgtgcaga gccgctgtaa caactattct
    34321 ggagcttgga aaggcttttc gtaggtcgac cagatcttcc ggcccctggt catcgtcggc
    34381 gacaaggcgg gcgacgacga gatccagctt agatgcgggc ggttgagcag aggcattgtg
    34441 taacgtggca ctatactgaa cgaagtgctc cattccaggc agcgccaact tgatgagccg
    34501 tttcagattc gagtcggcgt tgataatcag gatattggcc ataatccccc gacgcgaagt
    34561 gatggtggac agcgattgat catcgtgcga tcactcaaaa aaacgtcgca gatgtctgcc
    34621 cgttttggcg gagatggtgt ggtgaaggtg tgcctcctct cgataacctc gtggggttaa
    34681 accagttgct gatccagggc ataccgaatt aactctgcgg tcgttttcat tcccaatttc
    34741 gtgagcaacc gggcccgata ggtgctcacg gttttaatgc tcaggttgag gtgttcagca
    34801 atctcggtca ggcgccggcc ttgcgcaatc cactggagga cctcaatctc gcggtctgat
    34861 aggactgcag gcaggtcgtg ggcgcacggt gtgttgcggt ccagcatcgt gtcggagacc
    34921 tgttccgatt gcggcggggg gaaatagttc ccgccttgca gaattcggga cacggcagca
    34981 cgaagttgtt gcggacccgt gtctttggtc aagtatccca gtgcacctgc tcgaatggct
    35041 cgagcggcat agtgttgctc ggcgtatatg ctcacgatca gaatcggcag actgggttga
    35101 agagatttca caatgcgcaa gacttccaat ccaggcatgt ctggtaagtt caggtcgaga
    35161 atcaccagat cccaagctcc cgtccgaatt tggtcaatcg cgctatgggc cgagtcggtt
    35221 tcggtgatgt gaggcgttaa tccatcttcg gtgagaaaat cctgcaggaa ttccttgagg
    35281 ccgcgccgaa ggagggcatg gtcatcggcg agcaagatat tgagttctgt tttgacggcg
    35341 ggaagggtgt caaatgcatt cacagcagtg aattccccct atggtgtggt gctgagcatg
    35401 ggggcgccgt gcggaaacga agatccgtcc tggcccgcct gccattccaa tacggagcag
    35461 aaatgaggtc tgccggcgtg gaaacaggcg gaaagagagc gttcatgccc cttcatgacg
    35521 tctagtcgtg gcggcgttcc agcggacatc gaccctcagg ctacagccgg gacttctccg
    35581 gcgcaaattg gctggctgag acttccttcg attccttggc ccacgtgctc agtcgattca
    35641 atctcaggga aagctctaaa aattcctaga tttaagtagg ggaaaagtat cacgcgtaag
    35701 gggcgggcgc cagagctaaa gggccctgtt atggccctag agaattctcg cgactccagt
    35761 atgcttgtat tcccgaacga gcccgcgtgg cactcatctg cagagagacg ggaagagact
    35821 gctggggagg attacaggcg gtaaagggct ccgtatttcg cctccagata ccgtaagaac
    35881 ggttctggcg tcacgccgtt ccccgtgaca cgtcgagcta aatggtccgg cgtaaacata
    35941 cgcccccacc ggtgaatctt ttgttccagc cagtgccgaa gcggcaccaa ctgcccggcg
    36001 gcgatctgtt cttccaattg cgggagctcc agctttgctt gttcgaagaa ttgcaccgag
    36061 tagaggttgc ccaaggtgta ggtgggaaaa tacccgaagg ctcccatcga ccaatggacg
    36121 tcctgaagga cgccgtcggc gtcctgtgcc ggcgtgatcc ccaggtaggc ctccattctg
    36181 tcattccaca ggcttggcag gtcttccgga tgcgcccgtc tttcaatgag cgcctgctca
    36241 atttccacgc gcagcatgat gtgcagattg taggtcagct catcggcttc tacgcggatg
    36301 agcgaggggg tgacgcggtt aatcgcggca tagaattgat cgagaggcac cccggccaat
    36361 tgctgcggaa atgtgtgctg caaaatgggg taaaagcatt gccagaaggc tcgcgaccgg
    36421 cccacgcagt tctcccagag ccgcgattgg ctttcgtgaa aaccgagcga gacagactcg
    36481 ccgagcggcg atccgtaata gcgcgggtcc aggccctgat cgtacaagcc atggccgcct
    36541 tcgtggatgc agctgaacag gcatgacggc aaatccttct cgaagacccg cgtcgtcacg
    36601 cggacatccg tcgggtgaaa tgaggttgta aacggatggg ccgagagatc aaggcggcca
    36661 cgctcgaagt catatcccat ggcgacaagg acgagtcgcc cgaactcgat ctgcttcgtg
    36721 tgatcaaagc tatggtgaag gcaggtgtcg tcgatgcgca ccttgctggc ctgaactcgt
    36781 ttcaggagcg ggaccagccg cgcccgaagt tgtgcgaaga gcggcgtgag ttggcccact
    36841 gtggcgcctg gctcataggt atccagtagc gcgtcatagg gtgagtcccg gtatcccaag
    36901 tactgagtct cttcacattt gagatcgagg atggttctta acgaaggaag gaacttcgag
    36961 aattgacttt ctgctcgtgc cgtcacccag gcctgttgcg ccaacgaaca ttcacggctc
    37021 agacggatga caaaatcaga cgggagcttt ctcgcacgac tgaaatcacg ccaggtttcc
    37081 cgcaagagcg atcgcgacgg ttcatcccag tgttctgccg cttgcccggt ggaagggtcg
    37141 atccactcat tcaataggga ttcaatgtga ggtgacacaa gccgctcatg cgccagaccc
    37201 tccagcgtgg caatttgttc ggcgcgagct gccccgccac cagccggcat gtatgtttcc
    37261 tgatcccacg acagaacaga agctgcgctt tgaatccggc ggatttccag cagtcggttc
    37321 gttaacggtt ccaaggtctt caaggtcttc acttgtgcgc tctttctcca cgatgtccgt
    37381 ggtatgattc gcggccggtg cgaattgtcg cacaactgtc tcctggagtg aaactgatgg
    37441 ccgatctcgt gtcgccgaat atcgatgcct atgctgctgc ctgttctctc ccggaaacgc
    37501 cggttcgtcg ggcgctccgt gaggaaactg aacgtacaat ggagtacgcc cggatgttgg
    37561 tgggcccgct ggaaggggcg tttctgcatg tgatgacgcg cctggtgcga gctgagcggg
    37621 tcctggaaat cgggatgttc acgggctaca gcgctctctg ctttgccgaa gcattgcccg
    37681 atcagggtcg ggtgatcaca tgtgaggtgg atgaggactc tgctgcggta gcgcgtcgat
    37741 tctttgcgca gtcgccgcat ggccgtcgaa ttgagattcg tatggggccg gcactcgaga
    37801 ccatggcaga attgacggga cccttcgatc tgatttttat cgatgccgac aagctgaact
    37861 atgtgaacta ttaccggcga gcgatggaat tgctgtcgcc gcgcggggtc atcctggtcg
    37921 acaatgtgtt gtgggggggg gatgtcctca ccgatccgcc gccggatgac cggactgcgg
    37981 caatccagga cctcaaccgg atcgtggcgg ctgatccgcg tgtcacggcc gttctcgcga
    38041 ccattcgcga cgggatctgg gtcatcactc ccaaatcgtc gcactagcat cactggtcga
    38101 gacgttccga tcgaccactc gatataaatc ccaggaatcg cttgaatgga aaaaccgtcc
    38161 gcatccgccg cctatgcggc attgacgatt tcggccttgg tctggggtgg ttcaatcgtc
    38221 gggcaaaagc tggctcttgg tgcgttttca gccgtagaga cgtctctcat ccgcggcatc
    38281 ggcgcgctgg cgatcttaat cccgttgtgg tggtggactg aaggtaaccg tacaacttta
    38341 acggcccgcg acctcaaagt cctctcttta ttggggttgt tcgtattcgg taaccatctg
    38401 ttgaccctgt tcggccttcg ctatatcgga gcctcaacgg ctggggttct gatcggcgcc
    38461 agtccggcca ttacagcgct tctgtcttcc ctcttggtgc gtgatattcc attcaaccag
    38521 gtcgcaggcg gctgcgcggt ctcctttgct ggcgtcgcgc tcgtttcggg agtcggaggg
    38581 gatgctccca tcggcgatca tccctggtta ggtgggacgt tagtattgtt ggggctggtg
    38641 agctgggcgc tgtattccat cgggggccgt caggtgatga accgattgtc tccactgacc
    38701 gtcaactgga ccacgctgct gctctcgctg ttgttgcaga ttcctctcct ctcgaccgat
    38761 cagaaaatgt tggtgaccgg tacaagcgct gtgccggttt ccggttggct ggccgtgggg
    38821 tatctcattg tgttcgccac ggcgttgggc caacaggcat ggttatatgg cgttcaaggc
    38881 gtaggacctt cgcgggcagg ggtgttcgtg aatctcattc ccgtatcggc attgttgctc
    38941 tccgccgtga ttttgggtga agcaatcggg gcacgggaaa ttgcgggcat tgtcctcatt
    39001 ctgatgggcg tgtggttggt cggccgacaa accgagccct caaaacgtcg tggctagctc
    39061 ggtgcgctgc tcgttggtgt catcgatcca gccgttctgc tctgacgctc ttttcgttcc
    39121 ggctgtcgct tgaatgctcg ctctgtagta tgattgccgc caggttggca cctccacgat
    39181 cgctggaacg ttggcagcca gcccatcatt ctcctctcag tcttatcggg atgggtcccc
    39241 acgggaagcc acatgattga acatcatgat accggcttaa gcagagtgct cgtcatctcg
    39301 actcggccgg acggaatcat taccagtttc aatagcgcgg ccgagcgatt actcggagtc
    39361 ggcgccgatg cggttgtcgg taaagtgacg ccggctgcct ttcatgatcc ggatgaacta
    39421 cgccgccgaa gagaaaccct tgctgccggg ttaggaaagc caattcaaga ttcgttcggt
    39481 gtcctcgttg cccgggtcgg tattggacgg atgaccgaag acgagtggac gtatcttgat
    39541 cggagcggat atcggtttcc ggtatttctc tccgtgagcg ccattcctga tgaagctgga
    39601 cacgttctgg ggtactgctt tgtcgccaag gatcggcgcg ggcaactgca agccgaggca
    39661 ctgcttcgtc gtcaggcaaa actgctcgac ttggccaacg atgccattct ggttcgcgat
    39721 ctgcaagccg atacgattac gtattggaat gaaggcgccg tgcgattgta tggatggacg
    39781 gctgaagaag cgctgggggc gtatatccat gactttttga gcaccacctt tcctcttccg
    39841 ttagaggaga tcaaacagca atttctggag caggggctgt ggcgcggtga actcgtgcat
    39901 aagacacgtg ccgggaggga gattaccgtc tccagtcggt ggacgctgtt gcgcgatgct
    39961 gctgggatgc cgagcggcag tctggaattg aacacggata tcacggagca aaaacgggca
    40021 cagaaagcct tgaaggatgc gcatgaagaa ttggaattgc gggtcatgga gcgtacaacc
    40081 gacctccgtg atgctaatga gcggctacgg atcctttcgc gccgtctcat ggagattcag
    40141 gaattggagc gccgggcgat cgcccgcgat ctgcacgacg agatcggtca agcgttgacg
    40201 gccattaagt tgaatcttcg ggagcttcga acccatccca attgcgagcc tgtcgaggat
    40261 cagatcgcag acagtttgga aatattggct caggtgctgc agcgggttcg tagtttggcg
    40321 ttagatctac ggccatcttt gctggatgaa ttgggtttgg tgccggctct cagatggtac
    40381 gtcggcagac aagccgaacg cgcaggctgg caattgacgt tttctgctga cggcctgacc
    40441 agcaggccgc ctgcggaaat tgaaattgcc tgctttcggc ttgcgcaaga agcgttgacg
    40501 aatgtcgtgc gtcattccca ggcgaagaag gtagaagtgc tgctggagcg ctcttcccaa
    40561 gcgctgacga tggtgattcg cgacaatgga atcgggtttg atcgggatga ggtccgatcc
    40621 ggagccagcg tcggactctc gggtatggaa gagcgggttc aacttgtcgg gggagcgttc
    40681 agcattgaat caactccggc cgtgggcacg gagattcgtg cgtactttcc gcatggtgtc
    40741 gcgaccaaag gcactgagga ttcattggta tgaacaaaat cagggtattg ctggctgagg
    40801 atcatacatt ggtccgggcg ggatttcggg ctctcctgga gaaactggaa ggaattcagg
    40861 ttatcggtga agtgagcgat ggtcgcgacg cactgcgggt tgcgaaagag ctggatcccg
    40921 aagtggtgtt gatggacatc gccatgtccg gcatgaatgg tctggaagcg acgagtcgga
    40981 tgcggcagga atgtccccgc actaaagtgc tcatgctgac catgtacacg aatgaggagt
    41041 acctcaagga agccttacga gccggcgcat cggggtatct gctcaaagat gcggatcgtc
    41101 ccgaattaga gctggctatt aagaccgtct gtcgaggcga gacttatttg actccggcta
    41161 tggctaaatt caccctggat gcctactgtc ggcaggatga tgtgccgatg agcccgctga
    41221 gcaaactcac ggggcggcaa cgtgaaatcc ttcaattgat tgccgagggc tgctcgacca
    41281 agcagattgc ccaccgcctt gatttgagcg tcaaaactgt ggaaacccat cgagcccaat
    41341 tgatggaacg tctcgaaatc cacgatgttc ccggtcttgt aaggttagcc atcaggacgg
    41401 gattggtgcg atcagacaca tgagcaataa caagcggtta gaattatttc gcttccgcgc
    41461 gcattccgtt tgcgatcggg aataattttc gcggactcag ggaatcccct attacacccc
    41521 gggatgtggt gtgccatatt accgctgctt cacgggggtt cgttccttga atcatggctg
    41581 aacattcgcc ataccaaatt gggctgctgg tccttcctca tcaggttatt ctatgaaaaa
    41641 attgcgcgtg gctgtccttg gcacaggggc atttgctgaa gcgtgtcacg tgccaggatt
    41701 gcaatcgcat cccggcgcag aggtggtcgt cttatgcggg cgaaactcgg cgcgaactcg
    41761 agccatggcc gaccgtctcg gggtgccgga aatttcgctc gactacgaac aggtctgcgc
    41821 acgaaatgat atcgatgcgg taaccatcgc aactccaaac gtggcgcatg ccctccaggc
    41881 aaaggctgcg ttttcagccg ggaagcatgt cttttgtgaa aagccgctcg gcatgaatgt
    41941 tcaagaagtc attgaaatgg tccgagccgc agaacgtagc catgccatcc accaagtcgc
    42001 atttacctac cggtatttgc atggtgttca agaattgaaa tgtcggctgc ttaaaggcga
    42061 cataggcgaa ccgtatcatg tgcgtgtgca
//
LOCUS       DAOQEW010000007        42059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_176_length_42059_cov_8.956457, whole genome shotgun sequence.
ACCESSION   DAOQEW010000007 DAOQEW010000000
VERSION     DAOQEW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42059
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_42059_cov_8.956457"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PLS35_01660"
     CDS             <1..1316
                     /locus_tag="PLS35_01660"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNI17382.1"
                     /translation="ALSGCDKTIPGTVMALCRLNVPSLMIYGGSIMPGQFQGHDVTIQ
                     DVFEAVGKHASGKMTNAELKDLEDHACPGPGACGGQFTANTMAIAFEFLGMSPMGRNG
                     VPAMDVRKDDVAFECGKMVMDLLKKDIRPKQIITRRSIENAIAAVATTGGSTNAVLHL
                     LAVAREMGVRLTIDDFDKINRKVPLLADLKPGGHFTAADLYAAGGTTLVAKRLIDAKI
                     LHPDQITVSGRTIGEEAKAATEKPDQQVLRPLSKPIKPTGGLVILKGNLAPEGCVVKV
                     AGHSILHFSGPAKVYEREEDAFKAVQAGKIKAGDVVVIRYEGPSGGPGMREMLGVTAA
                     IVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAIKGGPIAAVKTGDVITFDIVKRRL
                     DVNITQKEMASRLKKVKHPAPRYLSGVMGKYARHVSSASEGAVTS"
     gene            1319..2224
                     /locus_tag="PLS35_01665"
     CDS             1319..2224
                     /locus_tag="PLS35_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17383.1"
                     /translation="MALRSRWSLAAALCAAACLLGPIKPHAGEPPQALCADWKSHHPE
                     WIWCDDFEIDRLSHYFEYDIRHGRFVREAGAGVGASTGMRAEFLPGDPHGGSLHLAFG
                     KTPSEYMKPVDAGTANYRDIYWRFDLRLQPGWTGGGADKLTRATILSSNRFAQAAIGH
                     LWSGAHPGSDPDRLYLDPASGTDEFGTLLTTTYNDFPRLRWLGAAGGQTALFNPAHIG
                     TWFCIEMHMKLNSRDAADGVLEYWIDGQLEAQRTNLNWIGMYNEYGINAVYLEQYWTS
                     VPFSKVQQRYFDNFVVSTARIGCAR"
     gene            2324..4468
                     /gene="ppk1"
                     /locus_tag="PLS35_01670"
     CDS             2324..4468
                     /gene="ppk1"
                     /locus_tag="PLS35_01670"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNI17384.1"
                     /translation="MALRSTASHIEPQSATPAPKVSSVRPVTSDLSRPEWFINRELSL
                     LEFNRRVLDLAKDPKVPLLERLKYLCIVSSNLDEFFEVRVAGLKEQVTHGVEQRGADG
                     LTPSELLARIAVLTHQLVQEQYVVLNQSLIPKLADERIRFLKRADWMPSHIRWMRRFF
                     SRELLPLLSPVGLDPAHPFPKLLNKSLNFLVTLEGTDAFGRPNGTAIVQVPRSLPRVI
                     HLPVRNAAWPHDFAFLSSVIHAFVHQLFPGMHVTGCYQFRVTRNSNLFVDEEDVDDLR
                     RALEGQLPDRRFGDEVRLEVADNCPPDLVYFLREQFHLDARDVYQCHGPVNLHRLMAV
                     PDLVDRPDLKFQPFTPGVPTTPVPSEDWFDAIRQSDILLHHPYQSFAPVTEFLRQAAT
                     DPHVLTIKQTLYRTGADSAIVQSLVDAARGGKEVTVVIELRARFDEEANIELAHDLEE
                     AGAHVVYGVVGLKTHAKMSLVLRREGRLLRSYTHLGTGNYHARNARLYTDFGLLTCDA
                     AIGRDVRTVFQQLTSLGQPGRLKHLLQSPFTLHTTLLKWIARETDRARQGKPAHIIAK
                     MNALLEPQIIRALYKASQAGVKIDLIVRGPCALRPGIAGLSEHIRVRSIIGRFLEHSR
                     IFYFLNDGDDRVFLSSADWMDRNFFRRIEVAFPVLDPILRHRLIEEGLRPYLEDNTHA
                     WILHRDGTYKRQTPGRRAARSAQQWLMNRLVT"
     gene            4603..4995
                     /locus_tag="PLS35_01675"
     CDS             4603..4995
                     /locus_tag="PLS35_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNI17385.1"
                     /translation="MAVAHVVRPRSRHQAFRSIRRQLLQDQRILLRQAASWRPPETEA
                     ELPADVLDIATCDRDLALGDLMRQRTYAKLQQITRALNRLDHASYGMCHLCRTDIPLP
                     RLRAQPDATLCVDCKAECEHRAMLRKQV"
     gene            complement(5702..6454)
                     /locus_tag="PLS35_01680"
     CDS             complement(5702..6454)
                     /locus_tag="PLS35_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17386.1"
                     /translation="MNRMVIAIVAVACLVNAACVSKPPKKQFQRLAGEFVSPVHITKP
                     VDQQSAVIGITIKSSAWVPNTEDRLYLVKIENDRDLFEGAKLIPTSIVSPPPGAGGGG
                     TVYVQNVPPGRYAAVAFSTSEKGFGKVREVTLFLLPKSVVKQSDTTVTAGSMHFMGEY
                     EVGASTMVLQENAADEVQAHYFEAFWGKPLAQVIADLQVLGTPAYFAVHTVNVKGGSR
                     DADAEARFQATAKRDLEPSWAPVIERARVPKK"
     gene            complement(6661..7923)
                     /locus_tag="PLS35_01685"
     CDS             complement(6661..7923)
                     /locus_tag="PLS35_01685"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17387.1"
                     /translation="MNMRSNALAFTIGLAILGTLSGCAEDHRWRAAMNDGNTAMRSGD
                     YTHAEESFVAARKEAEVLDPQGKRLAETLSQLGEVNRELGRYPRAEALFQEALAIRER
                     VYGPAHAETAASLTDLGELYRLQGLYTQSEALHQRAREIREKVFGPDHSKTAESLNNI
                     AVVYQDQRRFADAEPVLQRALAIFEKQLGPEHSLTAITRDNLAKMYQAQGQHPRAMPL
                     YQRALTIHEKAFGPNHPIVARNLENLGDTYRAQNQYPQAEALYQRAVSIFRKSIGSDH
                     VDTAEAMSRLGQLYELQGLYSQAEPLFQQAIAIREKQQGADNPQVAGELKNLASLYQS
                     QNKLDQSEDLYKQALVIYEQAVGYDREAYVKTLKSYASLLRRRQRSGEAERLEERAKS
                     VLKRLSLESQSQGKTAADLPLAPVSPVP"
     gene            complement(8152..10170)
                     /locus_tag="PLS35_01690"
     CDS             complement(8152..10170)
                     /locus_tag="PLS35_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNI17388.1"
                     /translation="MTNPSPNTPVSGPLRIALIGAGRHAQHHARAILRCPGVQLVAVA
                     DPSDAAQAAMRDIVPGIGCFKTPEELFASEKLHVVHIITPPATHAPIARMALKAGCHI
                     YVEKPFTESVEDAQQILDEASAKGLRVCAGHQLLYEPPTRVLTQYLRSIGRVVHVESY
                     FSFRTVRHAPGGRKVLRADHQLLDILPHPVYLLLQVLEQAGEGRTELLSLEVSQAGTV
                     HALVRRGGVTGTLIVTLEGRPVESYLRVIGKNGSLFADYVRSTTQRAIGPGSSGIDKL
                     FAPYRQAWQLLTGTTSAMASRFLKSQRSYPGLAELFSAFYESARTGNPSPLSPESLLE
                     TVRICERVAKALKVGEAKALAAAAPKPVESRGVLVTGGTGFLGKEIVRALLSRGRPVR
                     VVARREPSPWERIAGAEYVVADVATGAAAHLFKGVDTVIHAAAETAGGWPEHQRNSLD
                     ATEHMLRGAAAVGIKHFVHVSSLAVLAQGTGRPIPDDHPLEPDSKGSGPYVWGKLESE
                     RLAVQLGKDLGVSVKVIRPGALVDYRDFDPPGRLGKRLGNIFVAVGSPGDRLGVVDVG
                     FAGRFLGWMTDAWDMAPSPLNLLDPVSPTKRELLDRLRQANPDLSVFWLPRFVLIPLS
                     WVATLAQKVLRPGKPAIDVAKVFSVLPYDTSGIATLAPRVDSASELHR"
     gene            complement(10330..11325)
                     /locus_tag="PLS35_01695"
     CDS             complement(10330..11325)
                     /locus_tag="PLS35_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17389.1"
                     /translation="MDAASILIVEDEPVVAKDIQLSLQRLGYRVPATATSGEEAIRKA
                     GDTHPDLILMDIVLKGKMDGVETALQIQRRQDVPVIYLTAYADNHTLERAKVTSPAGY
                     MLKPYQANELRTTIELALHRAQHDRHMRARLRWIATTVRCIGDGIVTTDRGGRVTYMN
                     PAAEEFTKVTQDEAMGMSVTALMGFPDDGATQHGGNPANQAMAEMRLVTIDEATVMSR
                     QGEPRSIRGSVAPVVDDGGAVLGAVFVFHERVPGERHLGPSNVQAEAIWRMEERLGRP
                     QGIINLCSWCKRVPDQSGEWYDLATFIAERSAIQFNGGLCPECMDQCFPCNGKHK"
     gene            complement(11330..13162)
                     /locus_tag="PLS35_01700"
     CDS             complement(11330..13162)
                     /locus_tag="PLS35_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17390.1"
                     /translation="MTIPSSYFLGMSRSSYSILGVALALIAVIGAIDWWLPLGLTITT
                     LYVVPVLIASRIPYPRLTFWVAATASFVTILDLFHSPLYALTWVSAVNRAFALMVIWV
                     TALLCLRRQRDEAELLRINEDIEQQVQERTSDLAAANQELEVLRAEAVSELVAIVKSS
                     DDAIVGMTLTGIIQSWNRGAERVYGYSAEEVLGRPISVLCPPNRLDEVPTMLDRIARG
                     EHVRNVEMVQRRKQGERIDVSLTISPVKDSDGCVMGASAIARDVTVKKRIEAALRESE
                     ARFRMMADTAPVMVWMAGPDTHITFINKRWLEFTGRTMQEEIGDNWFTGIHVDDLERC
                     RKSYLDAFKSEQPFFLEYRLRRHDGEYRWIMDTGVPLFDEDGRFGGYIGTCMDLTERK
                     DMEDQLRRMLKEKESLLREVHHRVKNNLQVISSLLNLQSASIKDPVVNQLFRECQVRI
                     TSIALLHETLHRSNDLSRIKMGDYTRTLTGHLFRSYGVDPGLISLELNVDDVEFDIDT
                     GLTCGLIIDELVSNCLKHAFVESGRGTVHIDLLDHVDGTFTLCVSDDGIGIPTDGVLN
                     NPDSLGLELVTLLAEKLDGHTELRSGSGTEWRIRFQQLQYSERV"
     gene            complement(13680..14798)
                     /locus_tag="PLS35_01705"
     CDS             complement(13680..14798)
                     /locus_tag="PLS35_01705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNI17391.1"
                     /translation="MNIPLLDLKAQYQSMRSEILAAIEATCDEQGFILGPRVVALEQA
                     VATYVSSTHAVGVASGSDALLLALMALGVKAGDEVVTVPFTFFATAGAISRLGAKPVF
                     MDIRPDDFNMDPTLLERVITKRTKAIIPVHLFGQCADMGAIHDIARRHKIGVIEDACQ
                     AIGAAQNGKRAGVLGDLACFSFFPSKNLGGFGDAGMVTTNDKELAESIAMLRVHGSRV
                     RYVHEAIGINSRLDALQAAVLLVKLKRLDQWADGRRRNAARYHQLFTDAQLTDHVTLP
                     VTHPHNFHVFNQFTLRVRKRDELRAYLKDHGVGTEVYYPLPLHLQNCYRDLGYQKGAF
                     PQSERAAEEVLSIPIYAELTDEQLQYVVQMIAAFFRKR"
     gene            complement(14819..16009)
                     /locus_tag="PLS35_01710"
     CDS             complement(14819..16009)
                     /locus_tag="PLS35_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI17392.1"
                     /translation="MDVICHVITKLELGGAQEVAMRVVSGLDRRRFRPVLIAGPGGLL
                     TEEAHALEGVDVRLIPSLVREVHPLQDLQALFELVATFRRLRPKIVHTHSSKAGILGR
                     LAAWLAGVPCILHTIHGYGVTPAQPLWLQHLVIKLEWMVGRVTTHWIAVSQADRRQGL
                     EWELFSASKVSVVRPGIDPTPFAARIEQAERDRLRAALGVGPEQLLVGTVSCLKPQKS
                     PEDFIRVAALVCQRMPAAKCVLAGDGALRPQIEAMIQAQGLQDRVSLLGWRRDVASLL
                     KAFDVFVLTSQWEGLPCAILEARASRIPIVATRVGGSAEAIIEGIQGTLCPAGDVKAM
                     ASRVCQILGDERLRADLRNGAQELPEEFTIQETIKQYHSLYTYLLHATRPAEDVMKLQ
                     PNRL"
     gene            complement(16023..17333)
                     /locus_tag="PLS35_01715"
     CDS             complement(16023..17333)
                     /locus_tag="PLS35_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI17393.1"
                     /translation="MIVIVGAGLAGLSAAYHLRGMPYKILEREREVGGLCRSYVKDGF
                     TFDYTGHLLHFRQTAIKALVESLLPDRLQRHARKSFVFSHDTYTEYPFQVNTYGLPPE
                     VVRECLLGFIATLSRSPSTPPAENPSFKQWIIESLGEGIAKHFMVPFNEKLWQVPLDE
                     LTSDWVSWLVPKPDVKDVVSGALGIKDKAFGYNPSFQYPVSGGIKVLPEAFLPSVENL
                     TYDSELVEIETGRRRAVFRSAQGERIEEYDRIISTIPLPELVRRCVDLPASMRELAGA
                     LRWVSVYNVNLAVAREHVSDKHWIYFPEHRYPFYRAGFPMNFSPSMGQPGCSSLYVEI
                     SHQPTEKESETSLIERVRRGLEGAGVLQPTDELVMSDVKDLYYAYVLFDRYRSRAVKE
                     LLAELERRGISSIGRYGLWEHTSMEDAIAQGQQLAMRLRMRAAA"
     gene            complement(17330..18388)
                     /locus_tag="PLS35_01720"
     CDS             complement(17330..18388)
                     /locus_tag="PLS35_01720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNI17394.1"
                     /translation="MILLALTFLVAVLLSVYGVPIARRAALKFGIVDSPDGRLKHQGE
                     PVPYLGGLAIYLSFLVSLAFTFEFRQDVLGIVLSGTLIVMLGLIDDFGVLSPGTKLAG
                     QFLAVFVLIKSGIRIEIASLPDWVDILLTVFWMIGIINAFNLLDIMDGLSAGVGVISA
                     AFLCVVAILNGDQTIAFMLAALMGSLLGFLRYNWRPASIYMGDSGAMFIGLMLGALSM
                     IGKYTQGHSVSLLTPVLILGMPIFDTLFVMYIRFLRGLPVFLGSPDHIAIRLRHWGLS
                     VTHVVLLSYLGAAILGGIGLLVMAVPQDLALILSGVTVLCLAAAAVALTRVNVSSGVV
                     AVSPEAVPARSTERTGAV"
     gene            complement(18385..19524)
                     /locus_tag="PLS35_01725"
     CDS             complement(18385..19524)
                     /locus_tag="PLS35_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI17395.1"
                     /translation="MTVTYFSKSSSIGPSSRYRVYQYLPHLYNAGIECHVEPLFGPTY
                     FSILHIRSLMLQTVLKIPYVVARFLKRLGTLMMLGQRDLIVIEGQLFPYAPPLVERLL
                     CWLGYRLVIEMDDAIYLTRGHERKMPVLFRMATGVIVGNDRLAAYATQYSSKVTVVPT
                     VVDTERFAPRPLRPTAASHLLDDPITIVWIGLAYNLKYLNVLAPAIRRLQERYRVKLR
                     VVCSQPPVLDGLDVEFRSWEWRREVEDLQDATIGVMPLEDTEWARGKCALKLLQYLAV
                     GLPAVASPVGVNRDILVNGDNGFLASTEEEWYLRLESLCRDPQLRARMGQAGRRTVET
                     RFSLAVWGPTLADVYRAFAEDAPSRQMPRPVGLSVRGHGHSTGMR"
     gene            complement(19696..21522)
                     /gene="asnB"
                     /locus_tag="PLS35_01730"
     CDS             complement(19696..21522)
                     /gene="asnB"
                     /locus_tag="PLS35_01730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNI17396.1"
                     /translation="MCGIVGVCDRMEPQQAHHVLSRMLATMIARGPDGEGRFVDPHIA
                     MGMRRLAVIDVEGGGQPLFSRDGQVVAFQNGEIYNHAALRRELAQRGVECRTASDTEV
                     LAHGYDHWGMDGLLARLDGMYAIAILDQRNHQLHLARDRFGEKPLYYSVANGKFAYSS
                     NTTALAWLPWVDRDIDAGSVDRYLALHYVPGRRTMFKGIQRVLPGEHISISLDSLQII
                     HDRYYRPAMGPIEPVDTGTLVGRIEAAVTSRLVADVPVGVFLSGGIDSSIVAALAAKH
                     HPAIDTFSMGFKDARYDESPHAQAVARAIGSSHHHFTFDETAFHDLLPSVIDGLDEPL
                     GDQALLPTYWLCREAKRHVTVVLSGEGADELFAGYEYYGALLSRRSWRACLKAWVMGR
                     GPADELQRLCDNVWPVTPSGFPLVMDTAGRRQLLGGYAPDRDPWEEALRSTLQQSANG
                     LQRATLADVLTWLPDDLLVKLDRMAMANSLEGRAPFLSPVVAETALRLPESSRMTPVR
                     SKVALREVAARLLPPEIVHRRKQGFVLPMATWLKQWLRRVENLYEYFDLQRIAVFDGN
                     AAATLVERQLTSDRPNERLLFSLVMLAEWHHSFFRRIPPAKF"
     gene            complement(21539..22582)
                     /locus_tag="PLS35_01735"
     CDS             complement(21539..22582)
                     /locus_tag="PLS35_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17397.1"
                     /translation="MEQRLRSMLLPGMRRLGLVPLLRRLRAGFRSAGNAEPAGSGHPG
                     NDHDRVSDYWGQQFQAMRVDNSYWLNNKLVEEATYRLMTDTPRHWLGWLLNDYFAQRT
                     FDRSLSVCCGDGAHEIQLYTSGKVRFVSGVDISEGAIKQAAARFAEAGAPADCYRFEV
                     RDVNALQLEDQYDLIFSTGALHHTTNLEALLSTVERALTPNGYFVVVEFIGPNRFQWT
                     DRQIEIANQVLNVLDPAYLRDGRNTRFERPTIENMLACDPSEAVRSADVYPLVKKYFD
                     VRYERFYNGTLLHQLYPLLRTELANQGKRDFDSIVKLILLLEDLLIKGNVLPSDFVFL
                     ICQKPVPGSRRAA"
     gene            complement(22708..23847)
                     /locus_tag="PLS35_01740"
     CDS             complement(22708..23847)
                     /locus_tag="PLS35_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI17398.1"
                     /translation="MTVRFNLETPGGPQLNTINRFSGWYIPDHGRRPELYVACNGKRA
                     ASLEWGSVRPDVSSVYSTHSQAIISGFQGDLLMGEQEQAQAVEVVVLDAAGQDRELFR
                     RTYAVGPYRPPVIRDRSFNLFELLVCPECRSPLAKQDQGWMCPRCARLVPLKGGVPHF
                     LQEQGTPCLHVTEQTNTHPYSADVLDILGRHRAGLVLDFGAGHTPKELLRPHVVYLDA
                     VQYEWTDLVCTRARLPFRDHSFDAVVSQAVFEHLEDPHYTARELCRILRPGGIIHLDT
                     AFMQPLHGDPWHFFNMTQHGLRKVMAPFEEIRCGIKSYQHPSASLLMQFDAIAPFITS
                     RKWRNTIAKWRKELEAGAGEFDDALGEAGRTTLAAGFFFEGRRPQ"
     gene            complement(23844..26906)
                     /locus_tag="PLS35_01745"
     CDS             complement(23844..26906)
                     /locus_tag="PLS35_01745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI17399.1"
                     /translation="MNRLMQRLIKAIRFSRFAIVEGLRWYKRHGRLPRKAECADLFRK
                     AFFRFKILAQRISPAPKTIEPVLPPYDAWLKVNVWNRRMRDDLLDRLSRHAGRLPTIS
                     LVMPVHNPPLECLTKAIDSVRAQVCGEWELCIADDCSTNPAIRDELERFRAEDPRIRL
                     TYLERNLNISLATNRAADLATGEFLLFFDHDDELTPDAVGEAALYLAEHPEIDALYSD
                     DDKIDSAGKRYAPQFKPDWSPELLLSYMYMSHIFVVRRSLFETVGAMRQGFEGAQDYD
                     LALRVAERTGMVGHIPKVLYHWRALPGSTASSGAAKPASIEAGRRAVAEAFERRGIAV
                     RVTQPEFAAAGNLGIYCHEFPDDGPTVTILIPTKNQVTVLRQCVESLRATTYRQYEIV
                     IIDNESDDPETLAYLHSLPHRVIRLGNPSGRFNFAAINNRAVEQVTGEFILFLNNDTE
                     VKSPRWLSQMVGYAQMPGVGAVGAKLIFADGRIQHAGVIHGLYHGMAGPAFKLTPSWE
                     HGYLAYASVVRNYSAVTAACLLTSRRRFLDVGGFNEAEFGVAYNDVDYCYRLVDQGYR
                     CVYCPDAQLMHYEGYSRGFRDDPAEVAAFKQKYKRRQDPWYSPHLSLVDESFNLVPRA
                     VAARRQKPIPMVMTALSLNCEGAPWSQYELTKELVRRKVIQPIVFSPLDGPLRSLYEE
                     EGIPVMVDRHPLWGVTTLKEYEEAVKVFSSKCLSWGAELIYANTLQSFYAVAAAQIAG
                     IPSVWNPRESEPWQTYFDYLPDGVIQKAYDCFASPYRVVFVADATRDAYAALNTRHNF
                     TVVRNGLDQTRIQQTSEEWLPAQARQSIGACDGEVVILLLGTVCPRKGQQDLVKALAQ
                     LSPDLQKRVRCYIVGDRPSEYSAGLHALVAQLPSELRSRMHLVPETRQTARYYRAADL
                     FVCTSQLESYPRVVLEAMAYGLPIVTTPVFGIREQVREGVNALFYEAGDSGALADRLR
                     RLLQDEALRARLASKSASVLALGTDFDEMVSSYAEIFAEAWLSGGPLS"
     gene            complement(26999..27946)
                     /locus_tag="PLS35_01750"
     CDS             complement(26999..27946)
                     /locus_tag="PLS35_01750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17400.1"
                     /translation="MNKTVNESTELEATGERFLPEMQGTIALEHLHRYALAKDCAAGK
                     IVLDIACGEGYGSAVLAGTADRVYGVDIAPDAIAHARAIYQRPNLEYLVGECANIPLP
                     DCAVDLVVSFETIEHHTEHEAMFAEIKRVLRPGGVMVMSSPDKLEYTERSGHANAYHV
                     KELYAEEFQRLVERYFNRVAMLGQRVLYASAILPQGAPGEAVHYSRDKDSAIQAASGL
                     VRPCYQVAIASDAALPPVLSSLFEQAGESDPEIHALQHQLEHAGRRILGLEEEVAGLK
                     AAIELMMNSHSWKATRPLRAVANALRAVSGNRVEKSRRA"
     gene            complement(27943..28677)
                     /locus_tag="PLS35_01755"
     CDS             complement(27943..28677)
                     /locus_tag="PLS35_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI17401.1"
                     /translation="MNASMIPLPCKVCGGSTFSHHDVLWGDLIAQWGLSSREVDYINV
                     QQGTACTGCGSNVRSIALADALLRWTRATTTLQEWVHTPVARRCDVLEINEAGSLTGT
                     LSTLPGHRLVRYPEYDMMALPFEDRSFDVVLHSDTLEHVPDLMKGLRECRRVLRPGGA
                     CLFTVPVVVGRLTRGRKGLPLSVHGSEGCIDTALTVHTEFGADVWCQVLESGFDECRI
                     VPYRYPAGIALIAEVAESRPVTGGQW"
     gene            complement(28646..30019)
                     /locus_tag="PLS35_01760"
     CDS             complement(28646..30019)
                     /locus_tag="PLS35_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17402.1"
                     /translation="MSSEWAIRVRQLSKTYRVYDRPRDRGKQWILPPLQKCFGQSPSR
                     YYREVHAIEDVSFEVQKGETLGIIGRNGSGKSTLLQLIVGTLSPTSGTIETDGRIAAL
                     LELGAGFHPEFTGRENVFLNATLLGLSQADIDARLDDMLAFADIGSSIDQPVKTYSSG
                     MVVRLAFAVMAHVSADILVIDEALAVGDAFFVQKCIRFLRAFMERGTVLFVSHDSAAV
                     LSLCRTALWLEHGRIAGKGEAKVICDGYLHQPTPMEIPREKVAEGLPSSDPSDPAQPI
                     QNDSSNLAVLPHHETRSASHSRPAWIETITLCNANGRTVHTVTQREQVTLRVCCVTSV
                     GLASPIVGFLVRNRFGQSLFGANTCAAGNAAGHGVSPGTRFVAELEFVMPVLHPGDYA
                     MSIALADGTQEEHVQQHWIHDALAFTSAPSEWCFGLIAVEVMRTQICEIEAQDAQHER
                     LHDSIAL"
     gene            complement(30009..30833)
                     /locus_tag="PLS35_01765"
     CDS             complement(30009..30833)
                     /locus_tag="PLS35_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17403.1"
                     /translation="MVFQRYGLSPLIVLTSMSAHWNLIRQLTKREIASRYRGSLLGML
                     WAFLHPMVMLTVYTLVFRGAFGMRWGQDGESALDFGLLLFSGLIVHALFAESIHRAPY
                     LIVNHSNYVKKIVFPLEILAWTSLGSALFHALISALVLIAFYGFLHHDLHWTMLLVPL
                     LLLPLSLVTIGISWFLASAGVFVRDIGQASGPLTTVMLFLSPVFYPAGAFPEAYRILL
                     YANPLTFLITQAQDVLIWGRPPSWIGIGLYCAGSYLIAWCGLLWFQKTRKAFADVL"
     gene            complement(30950..31714)
                     /locus_tag="PLS35_01770"
     CDS             complement(30950..31714)
                     /locus_tag="PLS35_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNI17404.1"
                     /translation="MGAIGVIAVNAFRESLRDKILYNLVLFAGLLIGLSVLLADLSIT
                     EHHKVIADMGLAAINLIGVIIAIFVGISLVNKEIERRTIYTIMARPISRTFFIMGKYL
                     GLALTLFVNLAIMLAVFLLTLWLYHVPIQTSLFQAVALIFVEILVVTAIALFFSTFTS
                     TTLSAIFTLGLYVIGHLTADLRSMVANSESGAVKGVVDLLYYLCPNLEMLNIKGQAAV
                     GLVVAPEYLMLASLYGLLYASLLLTGACLVFQQRDF"
     gene            complement(31716..32687)
                     /locus_tag="PLS35_01775"
     CDS             complement(31716..32687)
                     /locus_tag="PLS35_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17405.1"
                     /translation="MDDIVTHNLTKTYASGWPGRPPFVALDGLSLTVGRGEIFGFLGP
                     NGAGKTTTLKILMGLVRPTSGTALLLGQPAGDVATRRRIGFLPESPYFYDYLTAEEFL
                     GFYGRLAGLNRSAITQRVTDLLELVGLVEARTRQLRKFSKGMLQRIGLAQALIHDPEL
                     IILDEPMTGLDPVGRKQVRDLILSLRDCGKTVFFSTHILHDVEMICDRVGIVMKGRLV
                     ASGRVDELVRQDHTRSVEVVCQQLKVEGNAFIHSLATRVLQQGQQCLIVLPSPDAVDA
                     LVGEIRRQGGQLLSITPHKASLEDLFFQEAAQATTRVLPHMTSGRAS"
     gene            complement(32700..33656)
                     /locus_tag="PLS35_01780"
     CDS             complement(32700..33656)
                     /locus_tag="PLS35_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17406.1"
                     /translation="MRGRTDIRSRLSHVLCVLVLLIGTSGVLHILEGERTAVARAEQL
                     AYLPKGEYFKLAVLGYRQVVADLIWLQAVQHIGAKRDTQLGHTWTYHAVDVLTDLDPT
                     FVPPYQATGLFLGVLVGRHDEGVAILRKGIRHNPANWQLPFLAGYISYYERCDASAAG
                     EFFQLAAHVPGSPAYLPQLAARMTVESGDPTAALEFLDRFSRSVADDRVREALFRRMK
                     EIVQEQDLRFLEESIRRYRLKYGQAPAKVEDLMLHGIIPRLPEDPLGGQYEIDGLTGI
                     VSASSKRDRLRIHEKVACRLGVASTPSTQADAIETLQPAVQQ"
     gene            complement(33662..35566)
                     /locus_tag="PLS35_01785"
     CDS             complement(33662..35566)
                     /locus_tag="PLS35_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI17407.1"
                     /translation="MIIRLVILAWLGGTLIQAIRAGRLIVPRLGARYVVLAFLGFAVA
                     ATIFSPYVHQSRQWLVMITGYAILFYLLVSVVDRWERVHTLTMMVIVMGLGEAAWTIV
                     QGVVWNVVRPSGTFFNPNFLAGYLTVSWAVLLSIAVYGYRKSAILYRSSVSPVFWWAG
                     MGSALGIVLIAMIFTQSRGGLVAWLAATVWILTARYGWKLAGTGAAVLLLAALIIPTP
                     FKERVLAEHEQNPVSYARWQMWQGAIAQMADHPLGIGLGLYQYTYPLYAFPVEGEITR
                     YGKVAHTPHNDYLQIGVEMGVGALLVFLAAIGLVMRDLLQVLQARLVRWQRSLILGIG
                     GGGIALLVHAALDSSLRESALAILLVLCAGLIVSVARITSGNTDALHAIPIQSRPLWG
                     MGAACLLLLVGAEVSRLGMAWLTFDKASRLAVAGDTEIAIQGFKNAIALDSGKSLYHH
                     GLGSVYARDFEASRDKQLFQLAHAEFKAAIELNPLDSRLLALLAQLYVSAAQTPSLSG
                     RLDEQQIFWLRAALRVYERAVRLAPFSAPYRYEQARLHWMLGERPLAEQQAKEAEQLE
                     PNFLPARALLARLSVDAGRIDDARRELQEIQVRQARYEQWNKNNIDRTFLSVDVAPLR
                     AAVEAKGLAG"
     gene            complement(35874..36395)
                     /locus_tag="PLS35_01790"
     CDS             complement(35874..36395)
                     /locus_tag="PLS35_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI17408.1"
                     /translation="MLKQVKGQKGFTLIELMIVVAIIGILAAIAIPNFLRYQAKSRQS
                     EAKTNLGAIFVAETAYLSENSRYGSFSEIGYALAGTTNRYTYRSPAILGNGASSNTVG
                     VDQIPCGAPVGCTVQSDASPAVPSLASSTAGSVGFTASAVASIDNDPTIDGWSVNDIK
                     GGLTQAVPDDVIS"
     gene            complement(36650..37177)
                     /locus_tag="PLS35_01795"
     CDS             complement(36650..37177)
                     /locus_tag="PLS35_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003111216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNI17409.1"
                     /translation="MVTESKQAGFTLIELMVVVVIVGILAAFAIPNFLRYRAQAMQAE
                     ARTNLAGIFVGEMSFFTERKEFGNFTDIGFAVAGPGTNRYTYRTGLGLGAGLGPNGGN
                     LCGSAGNCDTIQSEVPAAGAISFTGSVGIATTSSAGFTATAAADLDGDSTHDGWYVND
                     SKQGLSAADPNDVSS"
     gene            complement(37565..39421)
                     /locus_tag="PLS35_01800"
     CDS             complement(37565..39421)
                     /locus_tag="PLS35_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17410.1"
                     /translation="MQRLSEERRQSCTLCKPVGPKAAAVMLIALACVTAACQTAAPSR
                     STQPVVPAAAPPVSRPLSPASDSRASYHFLLGYQAELEQETEQAIKEYQLALQTDPSS
                     HYLKARLAVLYFTAGDVPAAVRFADEVADAPGLDAQMLGQIGGMYAAAGKPDKALRLF
                     NRAIEQEPQRSEHFFAKGLLLANQKQYAEAEETIRSGIKVSPDSAVGYYYLGRIGVEA
                     RDFDKATTHFEQAVTLNPAFEPAYVALGSVYEAKQDRGKAIDIYRRYLQGVNPKNREI
                     RHHLIRLQVSAKQYDEALRELQEMLEEDPSDLDAQLRMGLVYGEQKNYPKAIQQLTQI
                     LTVRPSELKVRDYLGYLYEETKDYAHAIEAYRHNLTLEPSYFEGHLHLGVLLYRTKQY
                     PEAIQHLKEASRLNPKQPEAYIVLGLSHFQVEQYEPSLQAFLEGIRHNPDNADLHFNA
                     GTAYDKLNRFDDVVKSMETTLTLDPHHADAMNYLGYSYAERGVKIEEAIALTKQAVAL
                     RPTNGYYVDSLAWAFFKKGLLSEALAEMKRAVALVGDDPVIYEHLGEIHLKQQHLSDG
                     REALLHALELDPSNDKLMQRFRELGLGDPAKEERIRQAIRRVTERKTAPSAQ"
     gene            complement(39503..40858)
                     /gene="dnaB"
                     /locus_tag="PLS35_01805"
     CDS             complement(39503..40858)
                     /gene="dnaB"
                     /locus_tag="PLS35_01805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNI17411.1"
                     /translation="MKSLSAVDLTAPRLPPQNIEAEQSVLGGILLDNTAMAKAMEVLT
                     DQEFYRTAHRKIYQAMLELSDRGEVIDQITLTECLKGRSELDAVGGSAYLAELVQAVP
                     TAANIRYHSKIVRDKALLRGLIETSTEVITRGYDGTAAVDELLDFAERSVFGLAQGKL
                     DRSFTQVNHIIKESLDVVDKLSKRKERVTGVPTGYIDLDDLTAGLQPSDLIIVAGRPS
                     MGKTSLALGMAQHAALHAGTVVGIFSLEMSKPQLVLRMLSSEARVDSHALRTGRLQKE
                     DWWRLAEAAGKLEQAPIFIDDSGAVTVQQMRGKARRLKAEKGLDLLIVDYLQLMQGKS
                     DSESRQQEISDISRSLKSLAKELNVPVIALSQLSRAVEARKPPVPMLADLRESGAIEQ
                     DADVVMFIYREEVYEPNTERKGIAEILVSKHRNGPIGKRDLFFHDRFAKFENLETRES
                     V"
     gene            complement(40855..41979)
                     /gene="hisZ"
                     /locus_tag="PLS35_01810"
     CDS             complement(40855..41979)
                     /gene="hisZ"
                     /locus_tag="PLS35_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNI17412.1"
                     /translation="MGSLSLKASASSTSLVAGRERSLIPVGMSTILPAAARRIRHLEQ
                     TLLAVLARGGYDEIILPMFEYLDVLAPGLEPELLEKCYQLVDRTTGRLMVLRPDATAQ
                     IARTVAMGMMGERLPLRLCYRTSVFRYEREHAGRDREIFQVGAELIGVDGVTGDAEVL
                     TLLLDCLSQVGLSAFKVAVGHVGFFTALLVRSGLSSEGQKRVEQAAARKDIPLLEELL
                     AREGLARSTGRIILEVLELCGGPEVLSRGRKLVGRDKVLLKPLDRLAQVYERLVSPGQ
                     ESVLIDLGEFRGFEYYDGIVFDVFAPGIGAELGGGGRYDHLMGRFGRTAASSGFALDV
                     DRLFRAIDSSVRPLPPDATRSQSKRRPATPPPSRRPRSRA"
BASE COUNT         9433 a        12771 c        11413 g         8442 t
ORIGIN      
        1 tcgccttgtc gggatgcgac aaaaccattc ccggcaccgt tatggcgctc tgccggttga
       61 atgtgccgtc gctcatgatt tacggcggat cgatcatgcc gggacagttc cagggacatg
      121 atgtcaccat ccaagacgtg tttgaagcgg tcggcaaaca cgcgtcgggc aagatgacga
      181 atgccgaact gaaagacctg gaagaccatg cctgcccggg acccggtgct tgcggcggcc
      241 agtttaccgc caacaccatg gccatcgcct tcgaatttct cggcatgtcg ccgatgggac
      301 gtaacggtgt cccggccatg gatgtgcgca aggacgatgt ggcgttcgag tgcggcaaaa
      361 tggtcatgga cctgcttaaa aaggacattc gccccaaaca gatcatcacc cgccgctcga
      421 tcgaaaatgc catcgccgcc gtcgcgacaa ccggcggttc gacgaatgcg gtcctgcatc
      481 tgctggccgt ggcgcgcgaa atgggtgtgc ggctcaccat cgacgacttc gataagatca
      541 atcgcaaggt gccgttgttg gccgatctga agccgggtgg gcacttcacc gcggcagatc
      601 tctatgcggc cggcggcacg accctggtgg cgaagcgcct gatcgatgcg aagatcctcc
      661 acccggacca gattaccgtc agcggacgga cgatcggcga agaggccaag gccgctacag
      721 aaaaacccga ccagcaggtg ttgcgcccgc tgtccaagcc gatcaagccg actggcggcc
      781 tggtcatcct caagggcaac ctggcacccg aaggctgcgt cgtcaaagtc gccggtcatt
      841 ccatcctgca tttcagcgga ccggccaagg tgtacgagcg cgaagaagat gccttcaaag
      901 ccgtgcaggc cgggaagatc aaggccggcg atgtcgtcgt cattcgatac gagggaccat
      961 ccggtggacc aggcatgcgg gaaatgctcg gcgtgacggc tgcgatcgtc ggtgcagggc
     1021 tgggcgattc agtcgcgctg ctcaccgatg gacggttctc cggcgcgacg cacggactga
     1081 tggccggaca tgtcgcccct gaagccatca agggcggtcc gatcgcagcc gtgaagaccg
     1141 gggatgtcat cacctttgac atcgtgaaac ggcgcttgga cgttaacatc acgcaaaagg
     1201 aaatggcctc gcggctgaag aaagtcaaac accctgcccc gcgctatctt tccggggtca
     1261 tgggaaagta cgcccgccac gtctcctccg cttccgaagg ggcggtcacc agctagccat
     1321 ggcactacgg tctcgatgga gtctcgcggc ggcgctctgc gccgccgcct gcctcctcgg
     1381 gccgatcaag ccccatgcag gcgagccacc ccaggctctg tgcgcagatt ggaagagtca
     1441 ccatccagaa tggatctggt gcgatgattt cgaaatcgac cgcctgagcc attacttcga
     1501 gtacgatatc cggcatggcc gcttcgtccg tgaagccgga gcgggagtcg gcgcctcgac
     1561 cggcatgcga gcagaattcc tgccgggtga tccgcatgga gggtcgctcc atctggcctt
     1621 cggcaagact ccaagcgagt atatgaagcc ggtcgacgcg ggaaccgcca attatcgcga
     1681 catctactgg cggttcgacc tacggctgca accgggctgg accggtggcg gagccgataa
     1741 gctgacgcga gcgacaatcc tttcgagcaa tcgatttgcg caggcagcca tcggccattt
     1801 gtggagcggc gctcatccgg gatcagaccc tgatcgcctc tacctcgatc ccgcatcggg
     1861 cacagatgaa ttcggtacgc tgctcacgac gacctacaac gatttccctc gcctacggtg
     1921 gcttggcgcg gccggcggcc agacggcgct gttcaatcca gcccacatcg gcacctggtt
     1981 ctgcatcgaa atgcacatga agctgaatag tcgggatgcc gcagatggtg tcctggaata
     2041 ttggatcgac gggcagctgg aagcgcaacg gaccaatctg aattggatcg gcatgtataa
     2101 tgagtacggg atcaatgccg tgtatctgga acagtattgg acgtcggtcc cgttcagcaa
     2161 ggtgcagcag cgctattttg acaattttgt ggtctccacg gctcgaattg gatgtgcccg
     2221 ataaacggga caccggcaag gcccccgcac agtgagcgga aaagttaacc gtttcttgat
     2281 ctgtcatcac accaggctgg tgtagactgc tcacccatta cgtatggcat tacgctccac
     2341 agcatcacac attgagcctc agtcagccac accggcaccg aaggtgtcat ccgtccgccc
     2401 ggtcacgagc gatctctccc gtcccgaatg gttcatcaat cgagagctca gccttctgga
     2461 attcaaccgc cgcgtgctcg atctggccaa ggacccaaag gtccctctgc tggaacgcct
     2521 caaatacctc tgcatcgtca gttccaatct cgatgaattt ttcgaggtgc gggtggccgg
     2581 ccttaaggaa caggtcacgc atggagtcga gcagcgtggg gccgacgggc tgacgccatc
     2641 ggagctcctg gctcgcatcg ccgtgctcac ccaccagttg gtgcaggaac agtacgtggt
     2701 cctgaaccag tcgctgatcc cgaaactcgc cgacgaacgg atccggtttc tgaaacgggc
     2761 cgactggatg ccatcgcaca ttcgctggat gcgccgcttc ttctcacgcg agctgcttcc
     2821 attgctgagt ccggtcgggc tggatcccgc gcatccgttt ccgaaactgc tcaataaaag
     2881 tttgaacttt ctggtgacgc tggaagggac cgacgcattc ggacgcccca acggcacggc
     2941 catcgtccag gttccgaggt ccctcccgcg cgtgattcac cttcctgtgc gcaacgccgc
     3001 ctggccgcat gattttgcgt tcctgtcctc ggtcatccat gcctttgtcc atcaactttt
     3061 ccccggcatg catgtcacgg ggtgctacca attccgcgtc acgcgaaaca gcaacctgtt
     3121 tgtcgacgaa gaagacgtgg acgacctccg ccgtgccctc gaaggtcaac tgccggaccg
     3181 tcgcttcggc gatgaggtgc ggctggaagt ggcggacaat tgcccgccgg atctggtcta
     3241 ttttctccgt gagcagtttc atctcgatgc gcgagacgtg tatcaatgcc atggaccggt
     3301 caatctccat cgactcatgg ccgtacccga tctcgtcgat cgccccgacc tgaagtttca
     3361 gccgttcaca cccggcgtgc cgaccactcc ggtgccgagc gaggattggt tcgacgcgat
     3421 ccggcagagc gacatcctgc tgcatcatcc ctatcaatca ttcgcgccgg tcaccgagtt
     3481 tcttcgtcaa gcagccacgg atccgcacgt acttacgatc aagcagacgc tatatcgaac
     3541 gggggctgat tcggccatcg tgcaatccct cgtggatgcc gcgcgcggcg gaaaagaagt
     3601 caccgtggta atcgaattgc gtgcccgttt cgacgaagaa gccaatattg agctggcaca
     3661 cgatttggaa gaagccggag cccatgtggt gtacggggtg gtgggactca agacgcatgc
     3721 gaagatgagc ctcgtgcttc gccgggaagg ccggctcctg cgcagctata cccatctcgg
     3781 caccggcaac tatcacgccc gcaatgcgcg gctctatacg gatttcggac tactgacttg
     3841 tgatgcggcc atcgggcggg atgtgcggac cgtctttcaa caactgacct ctctgggaca
     3901 accgggacgg ctcaaacacc tgctccaatc gccgttcacc ttgcatacta ccttgctcaa
     3961 atggatcgcc cgtgaaaccg accgtgccag gcaaggcaaa ccggcccata tcatcgccaa
     4021 gatgaatgcg ctgctggagc cccaaattat tcgcgcgttg tacaaggctt cgcaagccgg
     4081 agtcaaaatc gatttgatcg tccgtggacc ttgtgcctta cgacccggga tcgccggcct
     4141 ctcagaacat attcgtgtcc gctccatcat cggacgattt ctcgagcaca gccggatttt
     4201 ttacttcctc aatgacgggg acgacagagt gtttctctcc agcgcggact ggatggatcg
     4261 caattttttc cgacgaattg aagtcgcgtt tcctgtcctg gatcccatac ttcgccaccg
     4321 gctcatcgag gaaggcctcc gtccttacct ggaagataat acccatgcct ggattctgca
     4381 tcgagacggc acgtacaagc gccaaacgcc cggacgaaga gccgcacgtt cagcccaaca
     4441 atggctgatg aaccgactcg tcacttaagg ccttcgccaa tcctccaact ttttatcccc
     4501 cctgacggtt taaccccttc ttaacctgac tgtgatctgg ctgtcgtgtc cacacgatag
     4561 cctaatgacc gcaccagtca ctttaccgat ggaggggctc ccatggccgt cgcacatgtc
     4621 gtccgaccac gttcacgtca ccaggcgttc cgatccattc gccggcaatt gcttcaagac
     4681 caacgcatcc tcttgcggca agctgcgagc tggagaccac cggaaaccga agccgaactg
     4741 cccgccgatg ttcttgacat cgcgacatgc gatcgcgacc tggcgcttgg cgatctcatg
     4801 agacagcgga cctacgctaa actccaacaa attacgcggg cactcaatcg tctcgatcat
     4861 gcgtcctatg gaatgtgcca tctgtgccgg actgatattc ccctcccgcg tttgcgcgca
     4921 cagccggacg ccaccctttg cgtggactgc aaagcggaat gtgaacaccg cgcgatgctg
     4981 cgcaagcagg tctgaccatc cgcgagggat gggagttgag ctgcgccacc tcaccgcacc
     5041 tctctcccga gctgtccccc tctactgagc cggagcggga cagcccccct ccccattcaa
     5101 ctgaacgttc cgagtaggcg ctagcctgag ataccgcgag cggatgtgtt acataccgac
     5161 ccggcaatga ggatgatcgg cagcgaggat ggaatcgtcg tacgcgatta gcgacgtcga
     5221 gattggcgtt gacaacagga ccttataagg cgtcagcaga caggcgttcg agtcgctcac
     5281 ttatgcactc cgggcagagt gtcgcctgaa acctgcggac gtcaaccgta gagcgaaagt
     5341 cgttgtgaca ttcccggcag acgcggttgc tctggggtac gccatggcga aacgtcaaca
     5401 catcctgccg gcgcgaacga ggtcgataga tttgcaggcc tccctgtaca agttgcatgt
     5461 gatcctcctg cattggtggt gagatgcggc gacaatgact agcgtacagg aggaagatca
     5521 ccgcgacttc cggagtaggt aaacattgtg ttaacggaag aggcgatgat ggcgaagaga
     5581 ggcgggaaga tacggtgcgg atctggagct ttagcggggg gaccgatcgg atacattaca
     5641 aaaaaagagc cgcgcaccgt cctgacgatg cgcggccgta tctcgcgtcg ctccgctgtt
     5701 gttacttctt cggcactcga gcccgttcga tgaccggtgc ccacgaaggt tcaagatcgc
     5761 gcttcgccgt tgcctgaaaa cgggcttcgg catcggcatc acggcttcca cctttcacat
     5821 tgaccgtatg caccgcgaaa taggcggggg tgccaagaac ctgaagatcg gcaatcactt
     5881 gcgccaatgg ctttccccag aatgcttcga agtaatgggc ctgcacctcg tcggctgcat
     5941 tttcctgaag aaccatcgtc gacgcgccga cctcgtactc acccatgaaa tgcatgcttc
     6001 ccgccgtcac cgtggtatca ctttgcttca cgaccgactt cggcaacaga aagagggtca
     6061 cttcccgcac cttaccgaat cccttttccg atgtactgaa ggccaccgca gcataccggc
     6121 ccggcggcac attctgcaca tacaccgttc cacctccacc ggctccggga ggaggagata
     6181 cgatactggt cggaatgagt ttagcccctt cgaataaatc ccggtcattt tcgatcttca
     6241 ccagatacag gcgatcctct gtattcggca cccatgcgga actcttgatc gtgatgccga
     6301 ttacggcact ctgctgatcg accggcttgg tgatatggac gggcgaaacg aactctccgg
     6361 cgagccgctg aaactgtttt ttcgggggct tactcacaca ggctgcattg accagacacg
     6421 ccacggctac tatcgcgatg accattcgat tcatagaacg tcctctgggt gggtcgcact
     6481 taccggttcg gcggccacag tgtaggagat tctacgatgc gtctcaatcg atacttccga
     6541 aggggtgcgc gcgggcccgg ccggtccaag cagaatggag ggggtcggac caacgcgttg
     6601 agaggagaag caggagcacc gatggaggag tcctgagtcc aggaagctgg gggtacactc
     6661 ctaagggacc ggagacacag gcgcaagagg caggtccgcc gctgttttgc cctggctctg
     6721 gctttccaac gaaagacgtt tcaatacgct cttagcccgc tcctcaaggc gttcagcttc
     6781 gccggagcgt tggcggcgtc tcaataatga ggcatagctc ttcaacgttt tcacataggc
     6841 ttcacgatca tacccgacgg cctgctcgta gatgaccaac gcctgtttgt acaggtcctc
     6901 cgattgatcc agcttgttct gtgactggta gagcgacgcc agatttttca gttcacccgc
     6961 gacctgagga ttatcggcac cctgctgttt ttcccggatc gcaatcgcct gctgaaacaa
     7021 cggctctgcc tgcgaataca gtccctgcag ttcatacagt tgtcccaagc ggctcatggc
     7081 ctcagccgtg tccacgtggt cgctcccgat cgacttccga aagatgctca cggcacgctg
     7141 atagagcgct tcggcctgag gatattgatt ctgcgcccga taggtgtcac ccagattttc
     7201 caggtttctc gccacgatcg gatgattggg accaaaggcc ttctcatgaa tcgtgagggc
     7261 gcgctgatac agcggcatag cgcgaggatg ctgcccctgt gcctgataca ttttcgccag
     7321 attgtcccgg gtgatggccg tcaagctgtg ctccgggccg agctgtttct caaagatggc
     7381 aagggcccgc tgcagcactg gttccgcatc ggcgaagcgt cgttgatcct gatacaccac
     7441 ggcgatgtta ttcaggcttt ctgccgtttt gctgtgatcg gggccgaaca ccttttcacg
     7501 aatttctcta gcgcgttgat ggagcgcttc cgactgtgtg tacaatccct ggagccggta
     7561 gagctcaccc aggtccgtca aacttgcagc cgtctccgca tgagcaggtc catagacgcg
     7621 ctctctgatt gccaaggctt cctggaacag cgcttcggcg cggggatacc ggcctagttc
     7681 acgattcact tcgcccagct ggctcagcgt ttcggcgaga cgctttcctt ggggatcgag
     7741 aacttccgcc tctttacgcg cagccacaaa ggattcttcg gcgtgcgtat agtcgcctga
     7801 ccgcatcgcg gtattgccat cattcatcgc agcacgccag cgatggtctt ccgcgcaacc
     7861 ggagagggtg ccgagaatgg ccagaccaat ggtaaatgcc agcgcgttcg atcgcatgtt
     7921 catagtgtta actcttcgat ggtgaggcgg ttgtgtttgg agactacctt gcctgcggaa
     7981 tacccttaga cttacccgct ccgggggctc aaatcaaccc cgaggaagca actttttctt
     8041 gaagcgacgg ttcattcaat ttttcagaga cttagccatt ctgaagatcg gcgcatacac
     8101 tgtggaatag aggccacgaa taccgagcga agcacaccgt gagggtaaag ttcagcgatg
     8161 gagttccgaa gcagaatcga cgcgaggagc aagcgtagcg atgccggagg tatcgtaggg
     8221 caacacgctg aagactttgg ccacatcgat cgccggcttc ccggggcgca acactttttg
     8281 cgccagtgtc gccacccatg aaagcggtat caggacaaac ctgggcaacc aaaagacgct
     8341 caagtccgga ttggcctggc gaagacgatc cagcagctcc cgtttggtcg gagacacagg
     8401 gtcgagcaaa ttcagtggac tgggcgccat gtcccaggca tcggtcatcc agccgaggaa
     8461 tcgaccggca aatccgacat ccaccacacc aagccgatcg ccgggcgagc cgaccgccac
     8521 aaagatattg cccagacgct tgccgagacg gccgggagga tcgaaatcac gataatccac
     8581 cagagctccc gggcgaatga ccttcaccga caccccgagg tccttcccga gttgcaccgc
     8641 cagacgttcc gactccaact ttccccagac atagggcccc gaccccttgc tgtccggttc
     8701 caagggatga tcgtccggaa tcggccgtcc cgtgccttgg gccagcaccg cgaggctact
     8761 gacgtgaaca aagtgcttga taccgacggc agccgcgcca cgcagcatat gctcggtcgc
     8821 atccaaggaa ttacgctgat gctccggcca cccgccggcg gtttctgcag cggcatgaat
     8881 gaccgtatcc actcccttga agagatgagc ggcggctccg gttgccacat ctgctacgac
     8941 atattcggcg cccgcgatcc gctcccaggg agatggctcc cgccgcgcca ctacacgaac
     9001 ggggcgtccg cgggataaca gcgcacggac gatctccttt cccaagaagc ccgttccacc
     9061 ggtcaccagc acgccgcgac tctcgaccgg tttcggggcg gctgccgcca gggctttggc
     9121 ctcgccgacc ttcaatgctt tcgccacccg ttcgcagata cgcacagtct ccagcaaact
     9181 ctcgggcgac aacggcgacg gattgccggt gcgagctgat tcgtaaaacg ctgaaaacag
     9241 ctcggccaat cccggataac tccgctggct cttgagaaac cggctcgcca tcgcagaagt
     9301 ggtgccggtc aataattgcc acgcctgccg atagggtgcg aagagcttat caatgccgga
     9361 cgatcccggg ccgatggcgc gctgggtggt gctgcggacg taatcggcaa atagagatcc
     9421 gttcttcccg atcacgcgca gataactctc gaccgggcgt ccctccagcg tcacgatcaa
     9481 tgtaccggtg acaccgcccc ggcgcaccag cgcgtgcacg gtcccggcct gactgacttc
     9541 cagcgacaac agttccgtgc gcccttctcc ggcttgctcc agcacttgta ggagcaaata
     9601 gaccggatgc ggcaggatat cgagtaattg atgatcggcg cgaagcacct ttcgcccgcc
     9661 cggggcatgc cgaacggtcc ggaaggagaa ataactttcg acgtgaacaa cccgaccgat
     9721 cgagcgcaag tattgcgtca gcacgcgagt cggcggttcg taaagcagtt ggtggccggc
     9781 acagacgcga agaccctttg cgctggcctc atccaggatc tgctgggcgt cctcgacgga
     9841 ctcggtaaac ggtttctcca cataaatgtg gcatccggcc ttgagtgcca tacgcgcgat
     9901 cggggcatgg gtcgccggcg gggtgatgat atggacgacg tgcagttttt ccgaagcaaa
     9961 gagctcttca ggcgtcttaa agcagccgat acccggcacg atgtcgcgca tggccgcttg
    10021 agccgcatcg gaggggtcag ccacggccac caactgcaca ccgggacatc gtaagatggc
    10081 gcgggcatgg tgttgtgcgt ggcggccggc gccgatgagc gcgatccgca acgggcctga
    10141 tactggagtg ttggggcttg ggttcgtcac aagtagtctg cgaatgtgca gtgctactat
    10201 agtctattcg ggcgcgttct tgaagtcttc ttcgaaatta gtgcaaggag caggacaggc
    10261 gcgaaggctc gccgtcgacc cacggaatag cgctgagtga cagtacaggg attccaggag
    10321 cgagtttggc tacttatgtt ttccgttaca gggaaagcac tggtccatac actccggaca
    10381 gagcccgcca ttgaactgaa tcgcagaacg ctccgcaatg aatgtggcca agtcgtacca
    10441 ctcgccggac tgatccggca cccgtttgca ccacgaacac aaattaatga tcccctgcgg
    10501 ccgtccaagc cgctcctcca ttctccagat ggcttcagcc tgtacgttcg atgggccaag
    10561 gtgccgctca cccggaacgc gctcatgaaa gacgaacacc gccccgagca ccgcgccgcc
    10621 gtcatcgacg accggggcga cactcccgcg aatggaccgc ggctcgcctt gccgggacat
    10681 caccgtcgcc tcatcgatgg tcacgaggcg catctccgcc attgcctgat tggcaggatt
    10741 gccaccgtgt tgagtcgccc catcatcggg aaaccccatg agcgccgtca cactcatgcc
    10801 catggcttca tcctgggtca ccttggtaaa ttcttccgca gcgggattca tataagttac
    10861 ccggccgccg cgatccgtcg tgacgatccc gtctccgata cagcgcaccg tcgtcgcgat
    10921 ccatcgcagc cgtgcgcgca tgtgccggtc atgctgcgct cggtgcagag ccaactcaat
    10981 cgtcgttcgc agttcatttg cctgataggg tttgagcatg tagccggccg gcgacgtcac
    11041 ctttgctcgt tccagagtat ggttgtcggc ataggccgtc aggtagatga ccgggacatc
    11101 ctgccggcgc tgaatttgaa gagccgtttc gacgccgtcc attttccctt tcagcacaat
    11161 atccatcaga atgagatccg gatgcgtgtc gcccgctttg cggatggcct cctctcccga
    11221 ggttgccgtc gccgggaccc ggtacccgag ccgctgcaaa ctgagttgaa tatccttcgc
    11281 cacaaccggt tcgtcttcta cgatcaagat gctggctgcg tccatggcgt catacccttt
    11341 ctgaatattg cagttgctgg aagcggattc gccattccgt gcccgagccg cttcggagtt
    11401 ccgtatggcc gtccagtttc tcggccaaca gcgtgaccag ctccaatccc agcgaatcgg
    11461 gattgttcag caccccatcc gtgggaatgc cgatgccgtc atcgctcaca cacaaggtga
    11521 atgtcccgtc cacatggtcc aagagatcga tatgcacggt cccgcggccg ctctcgacga
    11581 acgcgtgctt caaacaattg gacaccagtt catcgatgat caggccgcac gtcagacccg
    11641 tgtcgatatc aaactcgaca tcgtctacat tcaactccaa actgatgagt cccggatcga
    11701 ctccgtagga acggaacaga tgcccggtca gggtgcgcgt ataatcgccc atcttgatgc
    11761 gcgagagatc gttcgaacgg tgtagcgttt catggagcag cgcgattgag gtgatacgga
    11821 cctggcactc tcggaacaac tgattcacca cggggtcctt gatagaggca gattgaagat
    11881 tcaacagact ggagatgacc tggagattat ttttgacccg atggtgcact tcccgcagca
    11941 aactctcctt ctctttgagc atgcgccgga gttgatcctc catatctttg cgctcggtga
    12001 gatccatgca ggtgccgata tagccgccga atcgaccatc ctcgtcgaac agtggcacac
    12061 ccgtatccat gatccatcga tactccccgt catggcgccg taggcggtac tcgagaaaga
    12121 acggctgttc cgacttgaat gcgtccagat aggacttccg gcaacgctcc aggtcgtcta
    12181 cgtgaatgcc ggtaaaccaa ttgtcaccga tctcttcctg catcgtgcgc ccggtgaact
    12241 ccagccaccg tttgttgata aaggtaatat gcgtgtccgg tcccgccatc cacaccatga
    12301 ccggagccgt gtcggccatc atccgaaatc gcgcttcgct ttcacgcaaa gccgcctcga
    12361 tgcgtttttt caccgtgacg tcgcgcgcaa tagccgacgc acccatgacg cagccatcgg
    12421 aatccttgac aggcgagatc gtcagcgaca catcaatacg ctctccctgt ttccggcgct
    12481 gcaccatctc cacattgcgc acatgctcac cgcgagcaat gcggtccaac atcgtcggca
    12541 cttcatctaa tcgattggga ggacagagca cggaaatcgg ccgccccaag acttcctcgg
    12601 cactatatcc atagacccgt tctgcacctc gattccaact ttgaataatg ccggtcaagg
    12661 tcatgccgac gatagcgtcg tcggacgatt tgacgattgc caccaactcc gacaccgctt
    12721 ccgcacgaag gacctcgagc tcttgattgg cagccgccag atccgaggtg cgttcctgca
    12781 cctgctgctc gatatcttca ttgatgcgca gcaactccgc ctcgtcgcgc tggcgacgca
    12841 gacacaacaa ggccgtcacc cagatcacca tcaaggcgaa ggctcgattc acggcgctca
    12901 cccacgtcaa cgcgtaaagc gggctgtgaa acaggtccag gatcgtgaca aaggacgcgg
    12961 tcgcagccac ccagaatgtg agacgtggat acggaatgcg agaggcaatg aggacaggca
    13021 ctacgtagag cgtagtgatc gtcaatccca gaggcagcca ccaatcgatg gcgccgatca
    13081 cggcaatcaa tgccagcgcc acgcccagaa tcgaatagct gctgcgggac atgcccagaa
    13141 agtatgaaga aggaattgtc ataattgtaa cgacgccgtt tcacgttccc tctgggtgta
    13201 ggagcaatac ctatgccttc caactcacac gcttcataac ggcatgcgtg aacacgtgct
    13261 gtcgaggaaa ggtacggacg tacagacagc ctcgccctgc tcggtgcgac ttcccggcta
    13321 accatgtatc cgcgacaggc ggcaggtgag gcgtttcacg cagctagtgc agcgcggctc
    13381 gcctccccgg gccacatctt ctgggagatt cgggtgtaca cctctgcctc atgaatgttc
    13441 agacttgaac ggcgcgcgat tcgtgcggtt gtcgtccatt caatgactga tcggagtggc
    13501 gcactaggca ggcacaatca tgcggttgcc cacgacgtat ctgtggagag aaggcgttgc
    13561 ggggcctgcg catggacgac acatgcacga gaccctgcgg cgttgaggag atcgaggatc
    13621 ggccgacgga acaggccgtt ggagtggaac gcgatcgacg ctggcgaagt ctgccggcgt
    13681 tatcgtttac ggaaaaacgc ggcaatcatc tgcacgacgt actgaagctg ctcatcggtc
    13741 agttccgcat aaatcggaat cgacaacact tcttccgcgg cacgctccga ctgcgggaaa
    13801 gcgcctttct ggtacccgag atcgcgataa caattttgca ggtgcagcgg caatggataa
    13861 tacacttccg ttccgacgcc atgatctttc aagtaggccc gcaattcatc gcgcttcctg
    13921 acacgtagcg tgaattgatt gaagacatgg aaattatgag gatgcgtcac aggcagagtc
    13981 acatgatcgg ttaattgtgc gtcggtaaat aactggtgat atcgcgctgc attccggcga
    14041 cgcccatcgg cccattgatc gagccgtttt aacttcacca acaagacggc tgcttggagc
    14101 gcgtccaacc ggctgttgat gccgatggct tcatgcacgt atcgcacacg gctcccgtgg
    14161 acccgcaaca tggcgatgga ttcggccaac tctttgtcat tcgtcgtcac catgccggca
    14221 tcaccaaacc cgccaagatt tttcgatggg aagaaactga aacaggccag gtctcccagc
    14281 acgccggcgc gcttcccgtt ttgcgcagcg ccgatcgcct ggcaggcgtc ttcaatgacg
    14341 ccgatcttgt gccgtcgcgc aatgtcatga atggcgccca tgtccgcaca ttgaccgaag
    14401 agatgcaccg ggataatggc tttggtccgt ttggtgatga ctcgttccag caacgtcggg
    14461 tccatattaa aatcatcggg acggatatcc atgaagaccg gtttggcacc caggcgtgaa
    14521 atagcccccg cggtggcaaa gaaggtaaac ggcaccgtga cgacttcatc gccggcttta
    14581 acacccaacg ccatcaatgc cagcagcaag gcatcgctgc cagaagccac gcccaccgca
    14641 tgcgtgctac tgacataggt cgccaccgcc tgttcgagcg ccaccacccg cggccccagg
    14701 ataaagccct gctcatcaca ggtcgcttcg atcgcggcca gaatttcact gcgcatggat
    14761 tggtactggg ctttcaaatc aagcagcgga atattcatcg tcttacccgg tttcctcttt
    14821 acaagcgatt gggctgcaat ttcatcacat cctctgccgg ccgcgtggcg tgcaggagat
    14881 acgtgtagag cgagtgatac tgtttaattg tttcttgaat cgtaaactct tccggcaact
    14941 cctgcgcccc gttccggaga tccgctcgaa gccgttcgtc acccaggatt tgacacacac
    15001 gactggccat ggctttgaca tcgccagccg gacagagggt tccctggatg ccctcaatga
    15061 tcgcttcggc ggagccccct actcgcgtgg cgacaatcgg aatccgactg gccctcgcct
    15121 ccagaattgc gcagggcaac ccttcccatt gagatgtcaa cacgaagaca tcaaacgcct
    15181 tcagcaatga ggccacatcg cgccgccagc cgagaagact cacccgatcc tgcagccctt
    15241 gcgcttgaat catcgcttcg atctgcggcc gcagtgcgcc gtctccggcc agcacacatt
    15301 ttgctgctgg catgcgctga cagacgagcg cggcgacgcg gataaagtct tcgggagatt
    15361 tctgcggttt caaacacgag actgttccca cgaggagctg ctccggcccc actcccagcg
    15421 cggctcgcag acggtcgcgc tccgcctgct caatccgggc ggcaaatggc gtcggatcaa
    15481 tgccaggccg gaccaccgac accttggagg cgctgaacag ctcccactcg agtccctgtc
    15541 ggcgatcagc ctgggagact gcgatccaat gggtggtcac tcgtccgacc atccactcaa
    15601 gcttgatcac cagatgttga agccacaacg gctgggcagg tgtcacacca tacccgtgta
    15661 tcgtgtgcag gatacagggg accccggcga gccaggcggc caaccgccct aggataccag
    15721 cttttgaact gtgggtgtgc acaatctttg gccgcaagcg tcgaaacgtc gcgaccagtt
    15781 caaacagcgc ctgcagatct tggaggggat ggacttctcg aacgagcgat gggatcaagc
    15841 ggacatcgac accttccagc gcatgcgctt cctcggtcag caagccgccg gggccggcaa
    15901 tcagcaccgg acggaatcgc cgacgatcca ggcccgacac cacccgcatc gccacttcct
    15961 gtgcgccgcc cagctcaagt ttggtgatga cgtgacagat tacatccatg cgattgacgc
    16021 cctcaggccg cggccctcat tcgcaaccgc atggcaagct gctgcccctg ggcaatcgcg
    16081 tcttccatcg acgtgtgttc ccacaatcca tatctcccga ttgacgaaat gccgcgtcgc
    16141 tccagctcag ccaacaactc cttcacggcg cggctgcggt accgatcgaa caacacatag
    16201 gcgtaataca agtccttcac atccgacatg accagctcat ctgtcggctg cagcacgccc
    16261 gccccttcca acccacggcg gacccgttcg atcagcgatg tctccgattc tttttccgtg
    16321 ggctggtgcg aaatctctac atacagagag ctgcaccctg gctgccccat ggacggagag
    16381 aaattcatgg ggaacccggc acggtagaat gggtaccggt gctccggaaa ataaatccaa
    16441 tgcttatccg acacatgttc ccgcgccacg gccagattga cgttatagac cgacacccac
    16501 cgcagcgcgc cggccagctc ccgcatcgac gctggaagat cgacgcaccg gcgcaccagc
    16561 tcgggtaaag gaatggtgga aatgatacgg tcatactcct caatgcgttc cccctgcgca
    16621 ctgcggaaaa ccgcgcgacg cctccccgtt tcgatctcca ccagttcgga atcgtacgtc
    16681 aggttctcca cggagggcag aaatgcctcc ggcaacacct tgatgccgcc gctcaccgga
    16741 tactgaaacg acgggttgta gccgaaagcc ttgtccttaa tacctaaggc gccgctgacg
    16801 acgtccttga catccggctt cggcacgagc caggacaccc agtccgaggt gagctcatcg
    16861 agcggcacct gccacaattt ttcattaaag ggcaccatga aatgtttggc gatcccttcg
    16921 ccgagacttt cgatgatcca ttgtttgaac gatgggttct cggcgggcgg cgtcgatggc
    16981 gaccgtgaca gcgtggcgat gaatcccagc aggcactccc gcaccacctc gggaggaagg
    17041 ccgtacgtgt tgacctgaaa cggatattcc gtataggtgt catgagaaaa gacaaatgat
    17101 ttgcgcgcat gccgctgcaa ccggtcggga agcaggcttt cgaccaacgc cttaatcgcg
    17161 gtctggcgaa aatggagcag atgtccggtg tagtcaaagg tgaagccgtc tttgacatac
    17221 gagcggcaaa gtcctccgac ctcgcgctcc cgctccagaa tcttgtacgg catgccgcgc
    17281 aagtgatagg ccgcgctcag ccctgccaat cctgccccga cgatgacaat catactgcgc
    17341 cggtcctttc tgtcgaacgt gccgggacgg cctcagggct caccgccacg accccgctgg
    17401 acacattgac tcgcgtcaga gccacagcag ccgcggccag acacaacacc gtgacaccgc
    17461 tcaagatgag ggcaaggtcc tgcgggacgg ccatgaccag cagcccgatc ccgcccaaga
    17521 tcgccgcccc gagataactg agcaacacga cgtgcgtgac cgacaacccc caatggcgca
    17581 accgaatcgc gatgtgatcg ggactgccga ggaataccgg caatccgcgg aggaatcgga
    17641 tgtacatgac gaacagcgtg tcgaaaatcg gcatgccgag gatcaagacc ggcgtcagca
    17701 acgagacgga atggccctgc gtgtatttgc caatcatcga caatgcgcca agcatcaagc
    17761 cgatgaacat cgcgcccgaa tcccccatgt agatggaagc cggccgccag ttgtagcgaa
    17821 gaaacccgag cagacttccc atcaaggccg ccagcatgaa cgcaatcgtc tgatcgccgt
    17881 tcagaatggc aaccacgcac agaaacgcgg cgctgatcac gccaaccccg gctgacaatc
    17941 catccatgat gtccaggaga ttaaaggcat tgatgatgcc gatcatccag aacaccgtga
    18001 gcaggatatc gacccaatcg ggaagcgatg caatctcgat acgaatgccg ctcttgatca
    18061 acacgaagac cgccaggaac tgcccggcca gcttcgtgcc cggcgacaac acaccgaagt
    18121 cgtcgatcaa tcccagcatg acgatcaacg ttcctgagag cacgatgccg agcacgtcct
    18181 gccggaattc gaaggtaaac gccaggctca cgagaaagga gaggtagatc gccaatccac
    18241 ccaaataggg caccggctca ccctggtgct tgagccgacc atccgggctg tcgacaatgc
    18301 cgaattttaa cgccgcacgg cgcgcgatag gcacgccata gacggacaac agcaccgcga
    18361 ccaggaatgt cagtgccagg aggatcatcg catacctgtg gaatggccat gtcctcgcac
    18421 agaaagaccg acaggccgcg gcatctgacg gctgggcgca tcctcggcaa acgcacgata
    18481 cacatcggcg agcgtaggtc cccataccgc caaggagaac cgggtctcca cggtccggcg
    18541 gcctgcctgc cccatgcgag cacgcaattg cggatcgcgg cacaatgact caaggcgcag
    18601 ataccactct tcttcggtcg aggcgagaaa tccattgtcc ccatttacaa gaatgtcgcg
    18661 attgaccccg acaggcgagg ccaccgccgg caatcccacc gcgagatact gcagcaactt
    18721 gagcgcacat ttgccccggg cccactctgt atcctccagc ggcatcacgc cgatggtggc
    18781 atcctggaga tcctcaactt cacgccgcca ctcccaggat cgaaactcga cgtccaaccc
    18841 gtcgagcacc ggtggctgag aacaaacgac gcgaagcttc acgcgatagc gctcttgcag
    18901 ccggcggatt gcaggcgcaa gcacgttcaa atatttgagg ttgtaagcca ggccgatcca
    18961 gacaatcgtg atagggtcat cgagcagatg ggatgcggcg gtcggtcgca agggcctcgg
    19021 agcaaatcgc tcagtgtcca ccaccgtagg caccaccgtg actttagagg aatattgggt
    19081 ggcgtaggcg gctaaccgat cgtttcccac aatgacgccc gttgccatcc tgaagagcac
    19141 aggcatcttc cgttcatggc cacgagtcag gtaaatcgcg tcatccatct cgatgaccaa
    19201 acgatagccc aaccagcaca acaaccgttc gaccagtgga ggcgcatacg gaaagagttg
    19261 cccttcgatg acgatcaggt cccgttggcc cagcatcatg agcgttccaa gccgtttgag
    19321 aaagcgcgcc acgacatacg gaatcttcag aacggtctgc agcatcaagg agcgaatgtg
    19381 gagaatcgaa aagtaggtgg gaccgaacag cggctccaca tggcactcga tgcccgcatt
    19441 gtacaaatgc ggcaagtatt gatacacgcg atagcggctg ctggggccaa tgctcgagct
    19501 cttagaaaaa taggtgaccg tcatggcgcg ccgtttcgcc ccgttgtctc ggaatatcca
    19561 ccggttgcgc aggcggaacc ggctagtcaa atagtaccgg agttctccgg caaggataga
    19621 tttttcgctg tttttgacgg gggcagagag gctcgatatc ggccgcgggc ctccgatagc
    19681 cgtcctcccg gttgtttaga atttcgcggg gggaatgcgg cggaaaaacg aatgatgcca
    19741 ttcagccagc atcacaagtg agaataacag acgctcattc ggacgatcag aagtcagttg
    19801 tcgttcaacc agagtggcgg cagcgttccc atcgaagaca gcaatacgct gcaggtcaaa
    19861 atattcatac aggttctcta ctcttcgtaa ccattgcttc aaccatgtcg ccatcgggag
    19921 gacaaatccc tgctttcttc ggtggacgat ttcgggcggc agcagtcgcg cggcgacctc
    19981 acggagtgcg accttgctgc gcaccggcgt cattcgactg gattcaggca gtcgaagcgc
    20041 ggtctcggcc actaccggcg acaaaaatgg cgcgcgcccc tcgagactgt tcgccatggc
    20101 catgcgatcc agtttcacca gaagatcatc gggcaaccat gtcaggacat cggcaagggt
    20161 ggctcgctga agaccgttcg ccgactgttg gagcgtggat cgcaacgcct cctcccacgg
    20221 atcgcgatcc ggcgcatacc cgccaagtag ctgccgccgg ccagccgtat ccatgacgag
    20281 cggaaaacct gatggcgtca ccggccatac gttatcgcac agccgttgaa gttcatccgc
    20341 tggacctcgc cccatcaccc aggctttcag gcatgcacgc cacgaccgac gcgacaacag
    20401 agcaccgtaa tactcatacc ctgcaaagag ctcgtctgca ccttccccgg acaacacgac
    20461 tgttacatgc cgcttggctt cccggcagag ccaataggtg gggagcaatg cctgatcgcc
    20521 gagtggttca tctaacccgt ctatgaccga gggcaacagg tcgtggaagg ccgtctcatc
    20581 aaatgtgaaa tgatggtgtg aggaaccgat ggcacgtgcc accgcctgag cgtgcggcga
    20641 ttcatcgtaa cgcgcatctt tgaaacccat cgaaaaggta tcgattgcgg gatgatgctt
    20701 cgcggcgagc gccgcgacaa tggaactatc gataccgcca gacagaaaaa cgccgacggg
    20761 gacatccgcg accaaccggc tggtaaccgc agcctcgatt cgaccaacga gcgtcccggt
    20821 atcgactggc tcaatcggcc ccatcgcggg gcggtaatac cggtcgtgga taatctggag
    20881 cgaatcaagc gagatactga tgtgttcacc gggcaggacg cgttgaatcc ctttaaacat
    20941 cgtacggcgg cccggcacgt agtgcagcgc gaggtaccgg tcgacactac ccgcatcaat
    21001 gtcacgatcg acccagggca accatgccaa agcggtcgta ttcgagctgt acgcaaactt
    21061 gccgtttgcg acgctatagt agaggggttt ttctccgaac cggtcacggg ccagatgcag
    21121 ctggtgatta cgctgatcca gaatggcgat cgcatacatg ccatcgagtc gcgctaacag
    21181 tccatccatg ccccaatgat catatccgtg cgccaacacc tcagtgtcgc ttgccgttcg
    21241 acattccaca ccacgttgtg ccagctccct tcggagcgca gcatggttat agatttctcc
    21301 gttctgaaag gctacgactt ggccgtctcg actgaaaagt ggctgtccgc caccttcgac
    21361 gtcgatcacc gctaaccggc gcatgcccat ggcgatatgc gggtcaacga accgaccttc
    21421 accgtcgggt ccacgcgcta tcatcgttgc cagcatacgc gacagcacat ggtgtgcctg
    21481 ctggggctcc atgcgatcac agacgccgac aatgccgcac atgcgttgat atccctggtt
    21541 atgctgctcg ccggctaccc ggcacaggtt tctggcagat gaggaacacg aagtctgacg
    21601 ggagcacgtt gcctttgatc aggaggtctt ccagcaggag gatcaatttc acaatcgaat
    21661 caaagtcccg cttcccctga ttcgccaact cagttcgtag gagcgggtac aattgatgga
    21721 gcagcgtgcc gttataaaag cgttcgtaac ggacatcgaa gtacttcttc accagcggat
    21781 atacgtcagc tgatcgcacg gcttctgacg gatcgcaggc gagcatattt tctatggtcg
    21841 gcctttcgaa tctggtattc ctgccgtccc gtaaataggc gggatcgagc acgtttagta
    21901 cctggttcgc aatctcaatc tgccggtcgg tccactgaaa ccgatttggc ccgatgaatt
    21961 ccaccaccac gaaatacccg ttcggcgtca aggcgcgctc caccgttgag aggagcgcct
    22021 cgagattggt cgtatgatgc agcgcgccgg tcgaaaaaat cagatcgtac tggtcctcca
    22081 gttgcagcgc gttgacatcc cgcacctcaa atcggtaaca gtccgccggg gctccggcct
    22141 cggcaaacct cgctgccgcc tgcttgatcg cgccttctga aatatccacc ccgctgacga
    22201 aacgcacctt gcccgatgtg tacagttgga tctcgtgcgc cccgtctcca caacatacgc
    22261 tgagagagcg atcgaacgtg cgttgggcaa aatagtcatt caggagccac cccaaccaat
    22321 gccggggagt atcggtcatc aatctatagg tcgcctcttc aaccagcttg ttattgagcc
    22381 agtacgaatt gtccactcgc atcgcttgaa actgttgtcc ccaatagtcg gatacccggt
    22441 catgatcgtt cccagggtgg ccggagccag cgggttcagc gtttccagca gagcggaagc
    22501 ccgcacggag cctcctcagc aggggcacca ggcccagccg cctcatcccc ggaagcaaca
    22561 tgctccgcaa tcgctgctcc atgaaacctg cctccatcat cccctaaatc ccactcacaa
    22621 gagacttatt tcgtcctgtc cggcattttc acggagcaac cgcccgcttc cggcacgccc
    22681 ccaagtggca gcccctggct acaaccatta ttgagggcgc cggccctcga aaaaaaaccc
    22741 tgctgccaag gtagttcgcc cggcctcccc gagtgcgtca tcgaactccc ctgccccggc
    22801 ctccaactct tttcgccact tggcgattgt gttacgccac tttcttgatg tgatgaacgg
    22861 cgcaatcgcg tcgaattgca tcaagagact cgcggaggga tgttggtagc tcttgatccc
    22921 gcaacggatt tcctcaaagg gagccatcac tttccgcaag ccatgctggg tcatattgaa
    22981 gaaatgccaa gggtctccgt gtaacggctg catgaaggcg gtgtcgagat gaatgatgcc
    23041 acccgggcgg agaattcgac agagctcgcg tgccgtgtaa tgcggatcct caaggtgctc
    23101 gaacacggcc tgactcacca ccgcatcgaa cgagtgatcg cgaaatggca gtcttgcccg
    23161 cgtgcagacc agatcggtcc attcatactg aaccgcatca aggtacacta catgcggacg
    23221 caacaattct ttcggcgtgt gtccggcccc gaaatccagt accaacccgg cccggtggcg
    23281 ccccaggatg tcgagcacat cggcagaata gggatgagta ttcgtctgct cggtgacatg
    23341 aaggcagggc gttccctgct cctgaagaaa gtgtggaacg ccccccttga ggggcacgag
    23401 cctggcacaa cgagggcaca tccagccctg gtcctgcttc gccagaggag accggcattc
    23461 cgggcatacg agaagctcaa acagattgaa cgaccggtcc cgtataacgg gcgggcgata
    23521 cggccccacc gcatatgtgc ggcgaaataa ttcgcgatcc tgccctgccg catcgagaac
    23581 cacgacctca acggcttgag cttgctcctg ctcccccatc aatagatcgc cttgaaaccc
    23641 gctgatgatc gcttgcgaat gagtcgaata gacggacgac acatccgggc ggacacttcc
    23701 ccactccagc gacgccgctc gttttccatt acaggccacg tacaactctg ggcggcgacc
    23761 atggtctgga atataccaac cagaaaaccg gttgatcgta ttcaattgcg gaccgccggg
    23821 cgtctccaga ttgaaccgta cggtcatgat aacggccctc cgctcagcca cgcctcggca
    23881 aagatctctg catagctgct caccatttca tcaaaatcag tcccgagcgc caggacgctg
    23941 gcactcttcg aggctaaacg agcccgcagt gcctcgtcct gaagtagacg ccgaagtcga
    24001 tcagccaggg cgccgctatc acccgcttca taaaacaaag cattcactcc ttctcgcacc
    24061 tgctcgcgaa ttccaaatac gggagtcgtc acaatcggga gaccataggc catcgcttcc
    24121 aataccactc gcgggtaact ttcgagctgc gatgtgcaca caaacaggtc cgcagctcga
    24181 taatatcgtg cggtttgacg cgtttctgga acgagatgca tccggctcct caactctgac
    24241 ggcagttgcg caacgagggc gtgcaatccc gcgctgtatt cacttggccg gtcccctacg
    24301 atgtaacagc gaacccgctt ctgcagatcg ggagacagtt gagccaaggc tttgacaaga
    24361 tcttgttgcc ccttcctggg acagaccgtt cccaggagta gtatcaccac ctcgccatca
    24421 catgcgccga tggactgacg ggcctgtgca ggaagccatt cttcgctcgt ctgttgaatg
    24481 cgtgtctggt cgaggccgtt ccgaacgacg gtaaaattgt gtctcgtatt gagcgccgca
    24541 taggcgtcgc gggtggcatc cgcgacaaag accacgcgat atggcgatgc aaaacaatcg
    24601 taggcttttt gaatgacccc gtcaggcaaa taatcaaaat atgtctgcca gggctcgctt
    24661 tcccgaggat tccatacgga ggggatgccg gcgatctgcg cagcagcgac ggcgtaaaaa
    24721 ctttggagcg tgttggcata tatgagttct gctccccacg acagacactt cgaggagaag
    24781 actttcacgg cttcctcata ttccttcagc gtcgtcacac cccagagcgg atgccgatcc
    24841 accatgaccg gaatgccctc ttcttcatac agggaccgca acggaccatc cagaggggaa
    24901 aacacaatgg gctgaatgac cttccgccgg accagctcct tcgtcaattc gtattggctc
    24961 cacggggctc cctcgcagtt caagcttaag gcggtcatca ccatcggaat cggcttctgt
    25021 cgccgcgctg cgacggcgcg cggcacgagg ttgaaactct catcgaccag ggacaaatgc
    25081 gggctatacc aggggtcctg ccgccgtttg tacttctgct taaacgcggc aacctcagca
    25141 ggatcgtcac ggaatcctct tgaataaccc tcatagtgca tgagttgcgc gtcggggcag
    25201 taaacgcagc gatatccttg gtcgaccagc cgatagcagt agtccacatc gttataagcg
    25261 acaccgaact cggcctcatt gaaacctccc acatcgagaa agcgccggcg agatgtcaac
    25321 aaacaagctg cggtgacggc cgaataattc cgcacaaccg aggcataggc caaataaccg
    25381 tgttcccacg agggcgtcag cttgaaggcg ggaccggcca tgccatggta aagcccatgg
    25441 atgactccag catgctgaat gcgaccgtcc gcaaagatga gcttggcacc aactgccccg
    25501 acccctggca tttgcgcgta tccaaccatt tggctcaacc agcgtgggga cttgacctcc
    25561 gtatcattgt tcaaaaacag aatgaactca ccggtgactt gctctacggc gcgattattg
    25621 atggcggcga aattaaaccg gccggaagga ttaccaagtc gaatgactcg atggggaagc
    25681 gagtgcagat acgccaacgt ctcagggtcg tcactctcat tgtcgatgat gacgatctcg
    25741 tactgtcgat aggtggtcgc gcgaagtgat tcgacgcatt gacgcagcac cgtgacctga
    25801 ttcttggttg gaatgagaat cgtcacggtc ggtccatcat ctggaaattc gtgacaatag
    25861 atccccaggt tccctgccgc cgcaaattcc ggttgcgtca cccgcaccgc aatgcctcgt
    25921 cgctcaaatg cttcggcaac agcccgccgt cctgcctcaa tgctggcagg cttcgctgcg
    25981 ccgctggagg ccgtcgagcc tggcaacgcc cgccagtgat agagcacttt cggaatatgc
    26041 ccgaccatgc ccgtccgctc cgccacccgc aacgcgagat cataatcctg agcgccttca
    26101 aatccctgcc tcatagcgcc cacggtctca aacaacgatc gtcgcacgac gaagatatga
    26161 ctcatgtaca tgtaggaaag cagcaactcc ggcgaccaat ccggtttgaa ttgcggcgca
    26221 taccgctttc ctgccgaatc gatcttgtca tcatcagaat aaagcgcatc aatctcaggg
    26281 tgttccgcca gatacagtgc cgcttcgccg accgcatccg gcgtcaactc atcatcgtga
    26341 tcgaaaaaca gcaaaaattc tcctgtcgcc agatctgcgg cgcgatttgt ggctagactg
    26401 atattgaggt tccgctccag gtaggtgaga cggatacgag ggtcttccgc gcggaaccgc
    26461 tccaattcat ctcgtatggc gggattcgta ctgcagtcgt cggcaatgca gagttcccat
    26521 tctccgcaca cctgcgcccg tactgaatcg atggctttgg tgagacactc aagtgggggg
    26581 ttatgcaccg gcataaccag cgaaatcgtc ggcaaacggc cggcatgccg gctcaatcgg
    26641 tcgagtaaat cgtcgcgcat tcgccggttc cagacattga ccttcagcca ggcgtcatag
    26701 ggagggagta ctggctcgat ggttttagga gccggcgaga ttcgttgcgc gaggattttg
    26761 aatcgaaaga aagctttccg gaatagatcg gcacattcgg ctttcctggg cagtcgacca
    26821 tgacgcttat accaccggag accttcgacg atggcgaagc gtgaaaaccg aatggctttg
    26881 atcaatcgct gcatgagacg attcatggct cacaacaagg cggtcggcaa gacgccagtt
    26941 actatgcctg gcccaccgcg cgataacggg atcgcgcagt ctgaatgtgt gcatgttttc
    27001 atgcgcggcg gctcttctcg actcggttcc cgctgacggc tcgtagcgca ttcgccacgg
    27061 cgcgaagcgg tctagtcgct ttccaggaat gcgaattcat catcagttcg atggccgcct
    27121 tgagccctgc cacctcttct tccagtccta gaattctgcg accggcatgc tcaagctgat
    27181 gttggagcgc atgaatttca ggatctgact ccccggcctg ttcaaacaag ctggacaaca
    27241 ctgggggcaa ggctgcatcc gaggcgatcg ccacttggta acagggccgc accaatccgc
    27301 tcgcagcctg aatagccgaa tctttgtcgc gggagtaatg aaccgcttcc cccggcgccc
    27361 cttgcggtag aattgccgag gcatacagca cccgctggcc gagcatggcc acacgattga
    27421 agtagcgctc aacgagtcgc tggaattctt ccgcatagag ctctttcaca tggtaggcat
    27481 tggcgtgacc ggagcgttcc gtgtattcca gcttatccgg actggacatc accataaccc
    27541 cgcccggtcg caaaacacgt ttgatttcgg caaacatggc ttcatgctct gtgtggtgtt
    27601 cgatcgtctc gaaactcacc accagatcga ccgcacagtc cggaagggga atattcgcgc
    27661 attcaccgac caggtactcg agattgggtc gctggtagat agctctggca tgggcaatcg
    27721 catccggcgc aatgtccact ccgtagacgc ggtcggcagt gccggcgagc acggcagagc
    27781 cgtacccttc gccgcaggcg atatcgagca caatttttcc ggcagcgcaa tccttcgcca
    27841 aggcatagcg atgcaaatgc tccagtgcga tggttccctg catctcagga agaaatcgct
    27901 ctccggtcgc ttcgagttca gttgattcat ttactgtctt gttcaccact gaccccctgt
    27961 taccggtctg gattcggcaa cctctgcaat gagcgcaatt cccgcgggat accgataggg
    28021 cacgatccga cattcatcaa agccgctctc caagacctga caccacacgt ctgcgccgaa
    28081 ttctgtatgt acagtcaagg cggtgtcgat gcacccttcg cttccgtgca cactcagcgg
    28141 caaccctttt cttccccgcg tgagccttcc caccaccaca ggtaccgtaa agagacaggc
    28201 tcctcctgga cgaaggaccc ggcggcactc ccgcaatcct ttcatcaggt ccggcacatg
    28261 ttccaaggta tcggaatgga gcacgacgtc gaatgaacgg tcttcgaacg gtaaagccat
    28321 catgtcgtac tcgggatacc gcaccaggcg atgccctgga agcgtcgaca gcgtgccggt
    28381 caacgatccg gcttcattga tctccaacac atcgcatcgg cgagccaccg gagtatggac
    28441 ccactcttgc aatgtggtcg tcgcgcgggt ccatcgcaat aaggcatcgg ccagggcgat
    28501 ggagcgcaca ttcgagccac agccggtgca cgcggttccc tgctggacgt tgatgtagtc
    28561 tacctcacgc gatgagaggc cccactgggc aatgaggtca ccccataaca cgtcatgatg
    28621 agaaaaggtg ctgccgccac acaccttaca aggcaatgga atcatggagg cgttcatgtt
    28681 gagcatcctg tgcttcgatt tcgcagatct gtgtgcgcat tacttctacg gcgatcagac
    28741 caaaacacca ttcggaagga gcagacgtga aagccaacgc atcatgaatc caatgttgct
    28801 ggacatgctc ctcctgcgtt ccgtcggcaa gcgcgatgct catggcatag tcgcccggat
    28861 gcagcaccgg catgacaaac tccagttcag ccacgaagcg cgtacccgga ctaacaccgt
    28921 gaccggcagc atttcccgcg gcacacgtat tcgcgccgaa cagagactgc ccgaagcggt
    28981 tgcgcaccag gaacccaacg atcggacttg ccaatccgac ggacgtgacg caacagacac
    29041 gcagcgtcac ctgctcacgc tgcgtgacgg tgtgaaccgt gcgaccgttg gcattgcaca
    29101 aggtgatggt ttcgatccat gctggccggc tatgcgatgc cgatctcgtc tcatgatgtg
    29161 gtaataccgc caagttcgac gagtcattct gtataggctg agccggatcc gaggggtctg
    29221 atgacggaag accttccgcc accttctccc gcggtatttc cataggggta ggctgatgca
    29281 ggtatccatc acagatcacc tttgcttccc ccttgcccgc gatccgtccg tgctccagcc
    29341 acaaggccgt gcgacagagg cttaggacgg ctgctgaatc gtgactgacg aacaacaccg
    29401 tcccccgttc cataaacgct ctaagaaagc ggatacactt ttgaacaaaa aatgcatcgc
    29461 caacagccaa cgcttcatca atcaccagga tgtcggcgct gacatgtgcc atcacggcaa
    29521 atgccaggcg gacaaccatt ccactcgaat acgttttgac cggctgatcg atcgatgacc
    29581 cgatgtcggc gaacgccagc atgtcatcca accgggcatc gatgtctgcc tgggacaaac
    29641 ccagcaaggt ggcattcaga aagacgttct cgcgccccgt aaactcagga tgaaatccag
    29701 cccctaattc caataacgcg gcgatgcgtc catcggtctc gatcgtgccg ctcgtcggac
    29761 tcaacgtgcc gacgatcaat tggagaagcg tcgacttgcc cgagccgttc cgtccgataa
    29821 tcccaagcgt ttcccccttc tgcacctcga aggacacatc ctcaatggca tgcacttcgc
    29881 gatagtatcg tgaaggagac tgcccaaagc atttctgcaa tggtggaagg atccattgtt
    29941 ttccacgatc gcgcgggcga tcgtacacgc ggtacgtctt agaaagttgg cggacacgga
    30001 tagcccactc agaggacatc ggcaaacgcc ttccgtgtct tttgaaacca gaggagcccg
    30061 caccaggcga tcagataact tcccgcacaa tagagaccga taccgatcca ggatggcggc
    30121 ctgccccaaa tgagcacatc ctgagcttgc gtgatgagaa atgtcagcgg attcgcatac
    30181 agcaagatcc ggtaggcctc aggaaacgcc ccagcgggat aaaacaccgg cgacagaaac
    30241 agcataacgg tcgtcaacgg accactcgcc tgaccaatgt cccgcacaaa gactcccgcg
    30301 gatgctagaa accacgagat accgatggtc accagagaca gcggcagcag gagaagcggc
    30361 accagcaaca tggtccagtg caggtcatga tgcagaaacc cataaaacgc gatcagcaca
    30421 agtgcgctga tcaacgcatg gaacaacgcc gaaccgagag atgtccacgc caggatttcc
    30481 agggggaaga cgattttttt gacgtaattg ctgtgattga cgatcaagta gggcgcccga
    30541 tgaatacttt ccgcaaacaa tgcatggacg atcaggcctg agaacagcag cagtccaaaa
    30601 tcgagcgcgc tctccccgtc ctgtccccac cgcattccaa aagcgcctcg aaagacaagg
    30661 gtatagaccg tgagcatgac catggggtgc aggaatgccc acaacattcc caggagtgat
    30721 cctcgatacc ggctggcaat ctcccgcttc gtcaattgac ggatcagatt ccagtgcgcg
    30781 gacatgctcg tgaggacgat caagggggag aggccgtaac gctggaagac catttcttac
    30841 ccttcagtaa gagcgcggca acaaatttcc aagactctgg gcgacacgca catagccggt
    30901 agccgacagg ctccatggct acgatgaacc acgcctctct cgcgatgact taaaaatccc
    30961 gctgctgaaa caccaggcag gcccccgtca gcaagagact ggcatacaac aggccataca
    31021 gtgacgcgag catgagatac tccggagcca cgacaagacc caccgccgct tgccctttga
    31081 tgttgagcat ttccagattt ggacagaggt agtacaacag gtccactact cccttcaccg
    31141 caccgctttc actattggcc accatcgatc gcaggtcagc cgtgagatgg ccgatgacat
    31201 agagacccag cgtaaaaatg gcgctcaatg tggtggaggt gaacgtcgaa aagaacagcg
    31261 cgatagccgt caccaccagg atttccacaa agatcagcgc gacagcttgg aaaagagacg
    31321 tctgaatcgg cacatgatag agccagagcg taagcagaaa caccgccagc atgatggcca
    31381 gattcacgaa gagcgtcagt gccaacccga gatacttccc catgatgaaa aaggtccggc
    31441 tgatgggcct cgccatgatc gtataaatcg tccgccgctc gatttcttta ttgaccaggc
    31501 tgatgccgac gaaaatagcg atgatcacgc caatcaaatt gatcgccgcc aggcccatat
    31561 ccgcaatgac cttatggtgc tcggtaatgg agagatccgc cagcagcact gatagcccga
    31621 tgagcaaccc ggcgaacagc acgagattgt ataaaatctt gtcccgcaaa ctttcacgga
    31681 acgcattgac agcaataacg ccgatcgccc ccatgtcagg acgccctccc actggtcata
    31741 tgcggcaaca ctcgcgtggt cgcctgcgcg gcctcttgaa aaaataaatc ctccaacgac
    31801 gctttgtgcg gagtgatgga cagcaactgt ccaccctggc gccgaatctc gcccacaagc
    31861 gcatcgaccg catcaggact gggcaaaaca atcagacatt gctgtccctg ctgcagcacg
    31921 cgggtggcca gggagtgaat gaacgcattc ccctcgacct tgagctgttg gcagaccact
    31981 tcgaccgatc gtgtatgatc ctggcgcacc aattcatcaa cccgtccgct ggccaccagt
    32041 cggcccttca tcacaatgcc gacacggtca caaatcatct caacatcgtg cagaatgtgt
    32101 gtgctgaaaa acaccgtttt gccgcaatcg cgcagactca aaatcagatc gcgtacctgt
    32161 tttcgtccga ccggatccaa tcctgtcatg ggctcatcga ggataatcaa ttccgggtcg
    32221 tgaatgaggg cttgcgcaag cccgatgcgt tgcagcattc ccttggaaaa cttccgcaac
    32281 tgtctggtgc gcgcttccac aagtccgacc agttcaagaa gatcggtcac tcgctgcgtg
    32341 atcgccgaac gattcaggcc ggccaaccgc ccgtagaatc cgagaaactc ttcggcagtg
    32401 agatagtcgt aaaagtaagg cgactccggc aaaaatccga ttcggcggcg cgttgcgaca
    32461 tcccccgccg gttgtcccag cagcagtgcc gttccactcg tggggcgcac cagtcccatc
    32521 aaaattttca gtgtggtcgt ttttccagcc ccgttcgggc cgagaaatcc aaaaatttct
    32581 ccacggccga cggtgagcga tagcccatcc agagccacaa atggaggtcg cccgggccag
    32641 cccgacgcgt aggttttggt cagattatgt gtgacgatat cgtccacggc actcctccgt
    32701 cattgctgca cagcgggctg cagcgtctct attgcatcgg cctgtgtcga gggcgtcgac
    32761 gcaactccta agcggcaggc tactttttca tgaatacgta gtcggtcccg ttttgaggac
    32821 gcactgacga tgccagtcag gccatcaatt tcatattgcc cgcctaaagg atcctcggga
    32881 agtcgcggaa tgatgccgtg cagcatcaga tcttccacct tcgcgggcgc ctggccatac
    32941 ttcagccgat accgtctgat gctttcttca agaaaccgga ggtcctgctc ttgaacaatc
    33001 tctttcattc gccgaaacag cgcttcgcga acccgatcat cggctacgct ccgggagaaa
    33061 cggtctaaaa attctaacgc cgcagtggga tctccacttt ccacagtcat gcgtgcggct
    33121 aattgaggaa ggtacgctgg agaaccgggc acgtgcgctg ccagctgaaa aaactctccg
    33181 gcggctgagg cgtcgcaccg ttcgtagtag gagatatagc cggcgagaaa cggtagttgc
    33241 caattggccg gattgtgccg gatacctttg cgtaagatag ccacaccttc gtcatggcgg
    33301 ccgaccaaca caccaaggaa gaggcccgta gcttgatagg gaggaacgaa ggtgggatcg
    33361 aggtccgtca ggacatctac ggcatgatag gtccaggtat ggcccaactg tgtgtcgcgc
    33421 ttcgcgccga tatgttggac agcctgtagc cagatcaggt ctgcgacgac ctgccgatac
    33481 ccgagaacgg ccagtttgaa atattctccc ttcgggagat acgccaattg ctcggccctt
    33541 gcgacagcgg tccgctctcc ctccagaata tggagcactc ccgatgtgcc gatcaacaaa
    33601 accaatacac acaacacatg actgaggcgc gacctgatgt ccgtacgacc tctcattggc
    33661 ttcacccggc aaggcctttt gcttcaacgg ctgcacgcaa cggcgcgaca tcgacactta
    33721 aaaatgtcct gtcgatatta tttttgttcc actgttcata tcgcgcctgc cgcacctgta
    33781 tttcctgcaa ctcccgcctc gcgtcatcga tgcggcccgc atcgaccgac aaacgagcca
    33841 gcaacgcgcg agcaggaaga aaattcggct ccaattgctc ggcttccttc gcctgctgct
    33901 cggctaacgg ccgctcgccg agcatccaat gaagacgcgc ctgttcatac cgatagggag
    33961 cagaaaatgg cgcgagccgg acggcccgtt catacacccg caacgccgca cgcaaccaaa
    34021 atatctgctg ttcgtctagg cggcctgata aagatggcgt ctgcgccgcc gacacataga
    34081 gctgcgcgag aagtgccaac aatcggctgt ccaacggatt taactcaatc gccgctttga
    34141 attccgcatg cgcaagctgg aacagctgct tatcgcgaga cgcttcgaag tctctcgcat
    34201 agaccgatcc gagaccatgg tgatagagcg atttgcctga atccaacgca atcgcgttct
    34261 tgaatccttg gatggcgatc tctgtgtctc ctgccacggc caaccgagat gccttatcga
    34321 atgtcagcca ggccatccct aatcgactga cttccgctcc gaccaacagt agcaaacagg
    34381 cggcacccat tccccacaag gggcgcgact gaatcgggat ggcatgcagg gcatctgtgt
    34441 tgccactggt tatccgagca actgatacga tcagtcccgc acagagaacg agcaggatcg
    34501 cgagggctga ttcacgcaaa ctggagtcca gcgcggcatg gacgagaagg gcgattcctc
    34561 cgcctcctat accgaggatg agacttctct gccaccgtac caatcttgct tggagcacct
    34621 gcagaaggtc ccgcatgacc aggcctatgg cggccagaaa gaccagcagc gcgcctacgc
    34681 ccatctccac tcctatttga agatagtcat tgtgaggagt gtgggcgact tttccgtatc
    34741 gggtaatttc cccttcgacc ggaaacgcat acaaaggata cgtatattgg tagagcccta
    34801 agccgattcc caatggatga tcggccatct gggctattgc gccttgccac atctgccaac
    34861 gggcatacga aacagggttc tgttcatgct cagcaagcac ccgttctttg aacggagtgg
    34921 ggatgataag cgccgcgagc agaagcacgg cggcgcccgt accggccaac ttccatccat
    34981 atcgggctgt caggatccac actgtggccg cgagccaggc caccagacct ccgcgtgact
    35041 gagtgaagat cattgcgatc aggacaatgc ccaacgctga tcccatgccg gcccaccaga
    35101 atacaggcga caccgaggac cgatacaata ttgcgctttt ccggtatccg tagacggcga
    35161 tgctcaatag aactgcccag ctgacggtca gatagccggc gaggaaattc gggttgaaaa
    35221 atgttccgct gggtctgacg acattccaca caacaccctg cacgatagtc caggcagctt
    35281 ctcctaatcc catcacgatg accatcatcg tcaacgtatg cacacgctcc caccggtcga
    35341 caacagagac cagcagataa aacaagatgg cgtatcccgt gatcatcact agccactgcc
    35401 gactttgatg gacatagggg gaaaaaatcg tggctgcgac agcaaagccc aggaacgcca
    35461 ggacgacata tctggctcca agccttggaa caattaaacg acctgcccgg attgcctgaa
    35521 tgagcgttcc accaagccag gccagaatca caagccgtat gatcatttgc gctggatgcg
    35581 tcgttccgcc ctcctgaagc ggcgaaaaaa tcatcagcgc gcacattacg atgactggca
    35641 tcgaagacag gaattgcata ggtgtgttcg cagacttgat gcgaccgata cctcctgctt
    35701 tatcagagac cggcaatttg gcaaagtgaa agtgaaattg aatactgcgg cgagagaaac
    35761 agggcaggaa agaaacaggc tgttcagaga atgggaagga tctggtcagc caaaagaaca
    35821 cgggggagga acattgttcc tcccccgcat cggagcagac ctgcgcgtga tcgttagctg
    35881 atcacgtcgt caggaacagc ctgggtcaga ccacccttga tatcgttgac tgaccaacca
    35941 tcgatggtcg ggtcattgtc aatgctggcc accgcggacg ccgtgaagcc gactgaacca
    36001 gcggtcgagc tagcgaggga cggcacagca ggagaagcat cactctgaac ggtgcaaccg
    36061 accggcgcgc cgcaaggaat ctgatcgacg cccacggtgt ttgacgatgc accgttaccc
    36121 aagatggcag ggctgcggta cgtgtaccgg ttggtcgtac cggcaagggc atagccgatt
    36181 tccgagaagc tgccatagcg cgagttttcg ctaaggtagg ctgtctcagc cacgaagatc
    36241 gcgcccaggt tcgtcttggc ttccgactgg cgcgacttgg cttggtagcg caagaagttc
    36301 ggaatggcga tggccgccaa gataccgata atcgccacga cgatcatcaa ctcaatcaac
    36361 gtaaaacctt tctgaccctt cacttgcttc aacataatgc ctccctttcc tattgttgcc
    36421 acttcatccg acacgttctt gattcctgtc acttcattcg gaaccgcgtg ttcacgcctg
    36481 acagatcttg ggaatagcag gttctatgcc caacacctcc ttcctgtagt tgcgtatctt
    36541 tttgcatatc ttccaatcac ttagatttag gatcgctttt gaactcctca cggctgcctg
    36601 aacgagattc ctttttccgt catcaggtga caagttttgg cacccgcacc taactcgata
    36661 catcattcgg gtcagcggca ctcagccctt gcttcgaatc gttgacgtac cacccgtcat
    36721 gcgtcgaatc tccatcaaga tccgctgcgg cggttgcggt gaaacctgcc gaagaggtgg
    36781 tggcgattcc tacgcttcca gtgaaactga tcgcgccagc agccggcact tcagattgaa
    36841 tcgtgtcgca gttgccggca gatccgcaaa gattgcctcc gttcggtccc aatccagccc
    36901 caaggccaag cccggttcga tacgtatacc gattcgttcc tggtccggcc accgcgaagc
    36961 cgatatccgt gaagtttccg aactctttcc gttccgtgaa gaaggacatt tcaccgacaa
    37021 aaattcccgc caggttcgtc cgtgcctcag cttgcatcgc ttgtgcgcgg tatcgaagaa
    37081 agttcggaat ggcgaatgca gccaagatcc ccacaatcac cactacgacc atgagctcga
    37141 tcagtgtgaa ccctgcttgc tttgattcag tcaccattgg gctcgaagac tttcgctctg
    37201 gaaacccatc ctgatgctcc gcccgtccgt actgccgaca ggccacgtat aagcaggccc
    37261 catgccagga gaggagcctt ttaactgttc ccgcataacc tgccgacaaa ccgaggcatt
    37321 tttgtctaga tttcacaatt ctggccctgt tcgctcaaac aggaagagga gccgagctag
    37381 gcaaagtgcc aaaaattgac tgaatcgaac aggtagagct cgttggctgg ggaggaagaa
    37441 ctgctgcgga agccgctgga ggactatgtg gaaacgaacg ggattggcaa tgaggtgatg
    37501 aacaatcggt ggcgatgccc tctcggtata gagggcacga ggcaactgta gcgtgagaca
    37561 agaattactg agccgaggga gccgtcttcc gctctgttac acgccggatg gcttggcgga
    37621 ttcgctcttc tttggcagga tcgcccaaac cgagctcacg aaaccgctgc atgagcttgt
    37681 cattcgaggg atcaagttcg agcgcgtgca ggagagcctc gcgaccatcg gagagatgtt
    37741 gctgcttcaa atggatttcg cccaaatgtt catagatgac cggatcatcg ccgacgagcg
    37801 ccaccgcccg cttcatttcg gccagagcct cgctcagcaa ccccttcttg aaaaaggccc
    37861 aggcgaggct atcgacgtaa tatccattcg taggccgtag cgcgacggcc tgcttggtca
    37921 gtgcgatggc ttcttcgatt ttgactcctc gttccgcata actgtacccg aggtaattca
    37981 tcgcatcagc gtggtgagga tccaacgtga gcgtggtctc catcgacttc accacatcgt
    38041 caaaacgatt caatttgtca taggcagtcc cggcgttgaa atgcaggtcg gcattatctg
    38101 gattgtgccg gattccttcc agaaaggctt ggagggaagg ctcatattgt tcgacttgaa
    38161 aatgcgacaa cccgagaacg atatacgcct caggctgctt ggggtttaag cggctggctt
    38221 ccttcagatg ctggatggcc tcgggatact gcttggtccg atacagcaag acccccaagt
    38281 gcagatgccc ctcgaaatac gagggttcca aggtcaaatt gtgtcggtac gcctcaatcg
    38341 cgtgggcgta atccttcgtt tcctcgtaga gatacccgag ataatctcgc accttgagct
    38401 cggaggggcg cacggtaagg atctgcgtca actgttgaat cgcttttgga taattcttct
    38461 gttccccgta cacaaggccc attcgcaact gagcatcgag atctgacgga tcctcctcca
    38521 gcatttcctg taactctcgc agcgcttcgt cgtactgctt cgccgacacc tgcagccgga
    38581 tgaggtgatg ccggatttca cgattttttg gattcacgcc ttgcaagtaa cgccggtaga
    38641 tatcgatggc cttcccgcga tcctgcttgg cttcatacac agaccccaat gcgacatagg
    38701 ccggttcaaa cgctggattc aacgtcacgg cctgctcgaa atgcgtcgta gccttatcga
    38761 aatcccgggc ctccacaccg attctgccca agtagtaata ccccactgcc gagtccgggc
    38821 tgaccttgat gccggagcgg atagtttctt cggcttcggc gtattgcttt tgattggcca
    38881 acaacagccc tttggcaaaa aaatgttcgc tgcgttgagg ttcctgctcg atggcacgat
    38941 tgaacagccg caaggccttg tccggctttc ctgccgccgc atacatcccg ccgatttgcc
    39001 cgagcatctg cgcatctagc cccggcgcat ccgcaacttc atcggcaaat cgcaccgcag
    39061 ccggcacatc acctgcggtg aagtagagca ccgctaatcg agccttgaga tagtgagaag
    39121 aggggtctgt ctgcagtgcc agctgatact ctttgatagc ctgttctgtc tcctgctcca
    39181 actcggcctg ataccccagc aggaagtgat aggaggcacg cgaatcagat gcgggagaca
    39241 aggggcgaga gaccggagga gccgcggcag ggacgaccgg ttgtgtggag cgtgacggcg
    39301 cggcggtctg gcacgcagcg gtcacacaag caagcgcgat cagcatgaca gctgcagcct
    39361 ttggtcccac gggtttacag agggtgcagg actgccgacg ctcttcactc aatcgttgca
    39421 caattcatcc gctcaatctg cagggggaat gttcggggta caggaaacga tgcggaatta
    39481 taccggcccg tgaaagaacg cgtcaaacgc tctcacgcgt ctctagattc tcaaacttgg
    39541 cgaatcggtc gtggaagaac aaatcgcgtt ttccgatcgg accattgcga tgtttgctga
    39601 cgagaatctc agcaatgccc ttccgctcag tgttcggctc atagacttct tcccgataga
    39661 taaacatcac cacgtcagcg tcctgctcga tggcgccgct ttcacgcaaa tcggccaaca
    39721 tcggcaccgg cggctttcta gcctcgacgg cccggctcaa ctgcgataag gcaatgacgg
    39781 gaacgttcag ctctttggca agactcttta aggagcggga gatgtcggaa atttcctgct
    39841 gccgcgactc ggaatcgctc ttgccctgca tgagctgcag atagtccacg atgagcaaat
    39901 caaggccctt ctcggccttg agccgtcggg cctttccgcg catctgctgc acggtcaccg
    39961 ctccggagtc atcgataaag atcggggcct gctccagctt gccggccgcc tcggccaatc
    40021 gccaccaatc ctccttttgc agtcttccgg tgcgaagcgc gtgcgaatca acacgagcct
    40081 cggaactcag catacgaaga accaattgcg gcttggacat ttcgaggctg aaaattccta
    40141 cgaccgttcc ggcatgcaag gcggcatgtt gggccatgcc gagggccaaa ctcgtctttc
    40201 ccatgcttgg ccgtccggcc acgatgatca agtctgacgg ctgcaacccg gccgtcagat
    40261 catccaaatc aatatagccg gtcggcaccc cggtcacccg ctctttccgc ttggacaact
    40321 tatccacgac atcaaggctt tccttgatga tgtggttgac ctgggtgaag gagcgatcga
    40381 gcttgccctg ggcgagcccg aacaccgagc gttcggcaaa atcgagcaac tcatcgaccg
    40441 ccgctgtacc gtcgtatccg cgggtaatga cttccgtaga ggtttcgatc agccctcgta
    40501 agagcgcttt atcccggacg atcttgctgt ggtaccgaat attcgcggcc gtagggacgg
    40561 cctgcaccag ctcggcaagg taggctgatc caccgaccgc atctaattcc gaacgtccct
    40621 tcaggcattc cgtcaacgtg atctggtcaa tcacttcgcc acgatcggac agttccagca
    40681 tggcctgata aatcttgcga tgagcagtcc gatagaactc ctgatccgtc aggacttcca
    40741 tcgccttggc catggccgta ttgtccaata gaattccgcc cagcacggac tgttcggctt
    40801 cgatgttctg tggcgggagt cgaggcgctg tcaggtcaac ggccgagagc gatttcatgc
    40861 tcggctcctt ggccgtcgtg acggaggagg cgtcgcaggc cgacgcttcg attgagatct
    40921 cgtcgcatcg ggcggaagcg ggcggaccga cgaatcgata gctcggaata gtcgatcgac
    40981 atccagagca aatcccgagg atgccgccgt gcgaccaaag cggcccatca agtgatcata
    41041 gcggccgcca ccgcccaatt ctgcgcctat ccccggagca aagacatcaa aaactatccc
    41101 gtcataatat tcaaagccgc gaaactctcc cagatcgatc aggactgatt cctgtcccgg
    41161 cgaaaccaac cgttcataga cctgcgccag tcgatcgagc ggtttcaata gcactttatc
    41221 gcgcccaacc agcttacgtc cgcgcgacag gacttcggga cctccgcata gttccaggac
    41281 ctccagaatg atccgtccag tggatcgggc cagtccctcg cgcgccagca attcctcgag
    41341 gagcgggatg tctttgcgag cagccgcttg ctccacgcgt ttctggcctt cgcttgagag
    41401 accggacctg acaagaagag cggtaaagaa gccgacatgc ccgaccgcca ctttaaaggc
    41461 gctgagtccc acctgactca aacagtccag cagaagtgtc agcacctcgg catccccggt
    41521 caccccgtca accccgatga gctccgcgcc gacttgaaaa atttcccggt cgcggccggc
    41581 atgctcgcgt tcgtagcgaa agaccgaggt gcgataacac aagcgcaacg gcagccgttc
    41641 gcccatcata cccatggcga cggttctggc gatctgtgcc gtcgcatccg ggcgcaacac
    41701 catcaggcgg cccgtcgttc gatcgaccag ctgataacac ttttcgagga gttccggttc
    41761 aagccctggc gcgaggacgt ccagatactc gaacatcggc agaatgattt cgtcgtaccc
    41821 gcctcgcgcc agaacggcca gcagagtctg ctcaaggtgg cgaatacgtc gagccgcggc
    41881 gggaaggatg gtgctcattc cgaccggaat aagcgaccgt tcccgacccg ccaccagcga
    41941 cgtggaggaa gcagaggcct tcaacgaaag ggagcccatg atcgactgcc gggcaatcta
    42001 cagacctttc gacaggtcgg cgacctttac ggaaaggatg tgcttgctgt tcgtaattt
//
LOCUS       DAOQEW010000008        42054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_177_length_42054_cov_8.383819, whole genome shotgun sequence.
ACCESSION   DAOQEW010000008 DAOQEW010000000
VERSION     DAOQEW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 42054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42054
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_42054_cov_8.383819"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLS35_01815"
     CDS             <1..192
                     /locus_tag="PLS35_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17413.1"
                     /translation="ANIAVLGADGTILAVNEASERFATANGFTENAHAGIRHVPDRTF
                     GCSRARVRTPQLVSAAMVS"
     gene            282..2714
                     /locus_tag="PLS35_01820"
     CDS             282..2714
                     /locus_tag="PLS35_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17414.1"
                     /translation="MHRSQAGLMAILEAAVDGIVVINERGIIESVNPAVTKLFGYTPD
                     RLLGQNVKMLMPDPYHTEHDQYLRNYLTSGLKKVIGIGREVLGRRSDGTSFPIHLAVN
                     EILVDGSRKFAGIVRDISSQILSEMQFRQVVDSAPNGLLMIDPHGTIMMVNKQVEAMF
                     GYSREELLGNRVEMLMPERFRSNHSTYRRGYFSGPTSRAMGVGRDLPGLRRDGSEFPV
                     EIGLNPIDTPQGRLALASIVDISGRKRMEDALAKAAEDLEEKNRELSETRDMALRAGQ
                     AKTDFLATMSHEIRTPMNGVLGMTTLLLDTTLTAEQRDYLQTLRSSSESLLRIINDIL
                     DFSKIEAGKFTIEHIPFDLRLTIEDTLDILAPTAQGRQLELVGLIDAQTPRTVVGDPG
                     RIRQILTNLVGNAIKFTEHGEVLIQVLQTDEDPGSVVLRFEVIDTGIGLSPEAQAKMF
                     QAFTQADSSTARKYGGTGLGLTICKRLVELMGGTIGLQSFAGQGTCVWFTIRLGTHTD
                     AAPAVPPIPSGLDLTGLRVCLVDDNATNRSLLQYHVSAWNMHHESAVDGPSALALLRR
                     GVEEGTPFDVAIIDMYMPEMNGLELCRLIKQDPALRKTHLIMLTASGQRGDSLAAQEA
                     GAAAYLTKPIRERHLADCIRLIFGRDNAKEQPAPFLTRHTITEAIAREAPRLLVVDDN
                     PVNQKVAVKMLEKLGCRVDLAGNGMEALAAICRHQYPLVFMDCQMPDLDGLETTRLLR
                     SQEKPGEHLPVIAMTANAMEGDRESCLKAGMDDFISKPIITSDLKKILARWVPQNQLE
                     GEQAASPETPSS"
     gene            2859..3416
                     /locus_tag="PLS35_01825"
     CDS             2859..3416
                     /locus_tag="PLS35_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNI17415.1"
                     /translation="MSHPSPSHTPSAPSTAAHRVEQLFEILIFGSRWIQAPLYGGLIV
                     AELLYAAKFLTELWHMIVHFREESETLFMLGVLSLIDVTMVANLLTMVIIGGYATFVS
                     KLDLETHPDRPEWLTHVDPGTIKIKLAASLVGISSIHLLKAFVDVTNENPEHIKWKIF
                     IHMTFLGSAIFLAYTDKLMQRDRKH"
     gene            3683..7681
                     /locus_tag="PLS35_01830"
     CDS             3683..7681
                     /locus_tag="PLS35_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNI17416.1"
                     /translation="MTTPGPEKDTYLTVANILGEETELLHGPFSDDHPLKVLERAAPT
                     EASDKRYLDELNRHIHACHPKRAGLCLSGGGIRSATFGLGVLQSLARLQLLDKFDFLS
                     TVSGGGYIGSWLTAWIHRHPRGLKGVIEDLNRPSKAGSEEASLPVRWLRNYSNYLSPH
                     LGFFSADSWTLLGIYLRNLHLNWMVLLPLLMMPLLAPRWIIALAQLNAKETSPSPISA
                     ASLLVIGIGLAVMALIYLHLCRPTLREFRRDTWWQTLESQRWFLIACLCPLLISVTCL
                     TTAWAWFRNGGGGADQFTWAHAVFGGALIHTVSWLFSAVALNRFRAFTRWLLLETLAV
                     AFTGALGGWLLRASFIKTCGQWNVASHAEWFATLAVPGVLTIFLLTATIFVGLASRFT
                     EEQDREWWGRTGSWVLIAMVMWSGLCAIVIFGPGLIHWMTPQVAASVGGLSGLLTLFL
                     GFSSRTTAQQQEERSTVTAVTDLAVRAAAPLFMVCVLIALALGTSWQLDLLAHHYHMH
                     LNHLDGPMQTGLGIGSDPWGHDQILHNTPWWLLFRFTATVVALGLLMSVFININRFSL
                     HAMYRNRLIRAYLGASRRQAERERTFNPFTGFDNLDNPSVSDLRFDDVRLARWFSAQE
                     QKQPTMQALLSYLQLSNPTDEQQEALCAQLPQDLRQTQACKRLSEAAHQLRLGPACVA
                     KLTALQMPDTAAGREASKWFSDLFIHKLHVQSPGPFHVINIALNLVKGDNLAWQQRKA
                     QSFTISPLHSGSWNLGYRRSEEYGANRYIGQPLSLGTALAISGAAASPNMGYHSSSAV
                     TFLLALFNIRLGWWLGNPGRAGADTYHQASPTLSIRPLLAEAFGLTDSCHPYVYLSDG
                     GHFENLGLYEMVLRRCHCILVVDAGCDPRLTFEDLGNAIRKIRIDLGIDIELNLDQIK
                     RAEGATTSNRHHAIGTIRYDKVDDNATAGTLIYLKPSLTGNEPSDVQDYAARHSAFPH
                     EPTSDQFFDEAQFESYRRLGEHIAQEVLRPALHRTEQDFSRLCHALRSHWVTTPPGMQ
                     ESFLGETDDLKDLERLLRDDPDLLRYDMEIYPELCSVFGLDCGNVALNPRAALHTCNL
                     QIQLMEQVYLAVKLEEFHGHALNRGWMNLFRRWTSSATFRMFWPTVCSMYSQPFVRFA
                     GQHFNLSIDQVAVLQPLEQTGDLSSLFRELLIEWASVPDYAETFMNALKRPLPLEEVT
                     DGPPPPLAAWQMRLPAIDSQAGPVGSGEILAVVAVTRLSVTGHRLALYGWVRPAYRGL
                     GLGHRLFGKAIDVLTEVYSGHNLIVDLGPDNAAWAGTSIRNVGWLRFYEQLGFTRDRS
                     DPKRFQMMRELS"
     gene            complement(7712..8095)
                     /locus_tag="PLS35_01835"
     CDS             complement(7712..8095)
                     /locus_tag="PLS35_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17417.1"
                     /translation="MPLILIADDNQQICGWLRAVLEAEGYRVIQASDGREAMATIERD
                     APDLMILDMYLPVQDGLETILMLHNVQSSVKILGISGQPLQGCDILKIAMAFGAHGVL
                     EKPFSRELLLRQVQTLLSSVQLPSV"
     gene            complement(8114..8794)
                     /locus_tag="PLS35_01840"
     CDS             complement(8114..8794)
                     /locus_tag="PLS35_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17418.1"
                     /translation="MEQQSVYRIVLIDDSRMVRLGIREQLLTATDFRVVGEAMSAVEG
                     MELVEQVKPDLVLLDSRLPDLSGVEACRTMVARNPAQRVLMLSIQDDPVVVQEAIVAG
                     ARGYLLKDAPVDTWLDAVRRVASGDVLFQPHLLGMVLREVHDMGNGLSSKSLAALSPQ
                     EHRLLPLVAEGKTNKEIASALALSDKTVKNYLANIFSKLGISRRTQAATLYARALPSR
                     SMSSGHYV"
     gene            complement(8950..9588)
                     /locus_tag="PLS35_01845"
     CDS             complement(8950..9588)
                     /locus_tag="PLS35_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17419.1"
                     /translation="MNKCRCLIADDHPVVRRGVRDLLEDEQLCSEILEAETGEEALDA
                     VRRQPWDLMILDIALPDKHGLDVLKEAKLLQPRLPVLMLSLYPEKEFAMRAIRAGASG
                     YLTKQSAPSELLAAVSRVLQGGRYITAALAEQLAQAFETGTEGELHARLSDRELQVLR
                     LLGQGKSVSMIGEELCLSVKTISTYRARILEKLSCESTGELIRYAIEARLID"
     gene            complement(9581..15541)
                     /locus_tag="PLS35_01850"
     CDS             complement(9581..15541)
                     /locus_tag="PLS35_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17420.1"
                     /translation="MGTHTLPPDETARVGALCRYRVLDSPPEADFDEMVQLAASIYKA
                     PIAAITFIDHDRQWVKAAVGLDTSQTDRPSGLCSFTIQGKDPMVIEDASADDRFSSHA
                     LVRGHPHVRFYCGAPLVTRDGYAVGTIAVMDSIPRTVKEDDLLTIRRLARQTAALLEL
                     RMLRSERSSVDSLSTDDQVETLTEAAFVIDRSGRILRANRGAERLLGVLASDLRGRLL
                     HECVHRPGDGDEEGQDGSCTLMHACLSGTAVLEQDDVFVNGDGSLLQVRWNHVPCWSG
                     GDWSGGIVAVRDLTIQKRNEEVLRLMAPTTRLTGTAFLQDLVETLVKTIGADYGFCLR
                     ALPEGRCAHSVAGWGPNGRLDPVDYALAGTPCEHVLHEGFCHYSSGVQALFPQDEVLV
                     HLSIESYMALTLPSPTGGPVGWIALLGRSPLKDAERAEALLRVAAGRAGSELGRHLAE
                     EQLKESEERYALALRGTSDGIWDWNVLAGQAVLSPRWKALLGFAEDELPDHESSFFQR
                     VHPEDVPRVKAEIERHFARRTPYDVQCRLLHKDGTYRWVQTRAQALWDDQGRAYRMVG
                     ASTDITERKQAEDVARRWQQVFDQAQLGLAHADLATNTFLAVNPAFARQCGYTVEELI
                     GRSIFFIYPPEEHAALEERIQRIHDTGGLTFESIHRRKDGSLFPVMVEVMLIRDADGV
                     PVSRVAYVKDITEAKQAEQARRASEERFRAAYRNATVGMSIADVGGRIQEVNQALCKI
                     LGYSEQELLANTFQSITHPDDLGQNLDRIQTLLSGRRVYDVFEKRYVRKDGGVVWAQV
                     GVSVIRDEDGTPLYTLAMVQDITERKHAQEALQLAKYTVDSATEAIYWVGADAELLDV
                     NDSACSMLGYSKDEFRRMRVHDINPNFQAETWPTFWAHTKERGRVSFESCHRAKDGRL
                     IPIDVNVNFVHYNGVECHFAFVRDITERKSAERALRASEERFHFAIEATNDGMWDWDM
                     RTDEVYYSPQWIRLLGYQSEEVLPTRAFFMTLLHPDDVARMMDVLQAHVDGRIPLKEI
                     EVRLRQKSGEFRWYLNRGKVVARAEDGRPLRMVGTITDISERKQSERERDEALSHLET
                     IMETVPDIIFALDLEGRVAKWNRKLETVTGYTREELQGKSALEMVPSDEAEKTAAAIR
                     EAYEKGYAELEGHLLAKDGRTMLYHWTGAPYRDLQGRVIGVTGVGRDITERKKVEQML
                     ASETRILEMMGSDAPLSAVLAEICLMIEGLSSGVLCSVLLLEEDEAHLRHGAGPSLPA
                     SYARILDGMAIGPARGSCGTAAFTGQQVVVTDIASHPFWADFRELALAHGLRACWSTP
                     IISAAGKILGTFAIYNQTPRQPTAEAPIVARASHLACLAIERKRTESALRLMQFSIQQ
                     AVDAVFLVSAEARILDVNEAACAMLGYTREELLSKTVHDIDTNFTRENWRARWQHLKV
                     RKSFSYESRHQKKDGTVIQTDTTINFLVHEGREYYCAFMRDITNRKRAEAQLHLTQFA
                     MERAADMVFWVDEAARLLLVNAAACERTGYTKDELLHMAVPDIDPNYQMEAWVRHWQE
                     LRETGRQRLETQHRSKSGEIYPVEVVANFVVFEGREYNFATVRDMSERQRTELRLRSS
                     EERFRLVAQATNDVLWDWDLVTNAHWWSPNAREKFGYDPQREPSIDAWTMRLHPDDKA
                     PILAMVQQAIESEVQTLAAEYRFLLADGTYGYFLDRAHIVRNEVGMAIRMIGAMIDVT
                     GPKRAYASLEEAYRRFQAMSQELQMVESNERRRLSRELHDELGQLLTSLKFDLASVKR
                     SVASRSKALGPRSQERLARALETTDLLFVRLRQIVRALRPPVLEELGLKAALEALIAD
                     VQARTRLRCSLVFEQGEGGPSRCPTLETAFYRIVQELLTNVIRHARATTVAIAITSGQ
                     QNWQMTVQDDGIGFDVAGLAPTGGFGLRGIRERVEILGGQVDILSSSETGTRVHVVIP
                     VSSEPEPACETQHETTASRRRRRKTVDE"
     gene            complement(15688..18201)
                     /locus_tag="PLS35_01855"
     CDS             complement(15688..18201)
                     /locus_tag="PLS35_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI17421.1"
                     /translation="MPAKKGSTARPSAAANQGQPTEEFEKLGVFYLGRPYALATKQSK
                     PGWLLYDSKDLVTHAVCVGMTGSGKTGLCVGLLEEAAIDGIPAIVIDPKGDLSNLLLT
                     FPQLRGEDFAPWINEDDARKKGLSPADYATQQAALWQKGLGEWGQSGERIQKLRDAAD
                     VVIYTPGSNAGLPVSILKSFAAPSPEFLDDAELLRERISTTVTSVLGLIGLQADPIKS
                     REHILLSSILDSAWRAGRDLDLAALIHQIQTPPMTKVGVLDVESFFPSKERFALAMQL
                     NNLLAAPGFAAWMEGEALDVGQILYGPSGKPRIAIFSIAHLNDAERMFFVSLLLNQTL
                     GWVRSQSGTTSLRAILYMDEIFGYFPPVANPPSKAPLLTLLKQARAFGLGVVLATQNP
                     VDLDYKGLANTGTWFIGRLQTERDKARVIEGLEGAAASSGKKFDRQQMEQILAGLGNR
                     VFLLNNVHEDAPEVFQTRWTLSYLRGPLTRTQIKQLMDPVKRQLPARTSRQDELGAAG
                     ASGSTSAASRTTASRPMLPPDVPQYFLPLRGTQPSGATLIYQPMVLGAAQVRFTDNKA
                     GIEATNDVTRLAAISSGVVPVEWEQSTAADVQVADLEREPAAAAEFAALPGAASKGKQ
                     YDSWKSDLSGWLFRTQKLELWRSPTSNTLSKPGESERDFRVRLQQVGREERDRLSDEL
                     RKKYAPKITTLRDRVRRAEQAREKQQNESRSSQVQAVISVGASILGAFLGRKTLSATN
                     IGRATTAIRSAGRVMKESQEVGHAEENVATLQQQLTDLEAQFKSESDALMAATDPLTE
                     KLDQISLKPTKSNIIVKLVGLAWVPAWRGADGTSAPAWL"
     gene            18425..18697
                     /locus_tag="PLS35_01860"
     CDS             18425..18697
                     /locus_tag="PLS35_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17422.1"
                     /translation="MSRGWALGIAVLLTGCGPHYTIPNYDTNEIEKVISSGRSPQGCI
                     ENLKEDAKNLNVDVRLTDVQQQPSSGPISWIYTNTYMCTGKVVAKR"
     gene            18716..19537
                     /locus_tag="PLS35_01865"
     CDS             18716..19537
                     /locus_tag="PLS35_01865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNI17423.1"
                     /translation="MNTVSMRHSATNEGLGGRRPADAPLTLRFLVTFDDGPHPNTGKV
                     LRHLARNPVQPHIKGIFFVQTRHPKRGGSSDGRAMLAIEQAEGHLLGLHTGTTKGHLS
                     HTRMSPAELERSLLNGKEDLDQITGKPTMLVRPPYWLFNASTFDCYGRHRLHMLLSDV
                     KAFDGVNWGIHLLRRLNFRSQLAGLHARFLRGTLPVVNHTIPIVVTFHDTNSFTAEHL
                     GDYLSLLVEEAARLGLPLHSKPFIDDPDELLAAALRCAKTSRGTERNPALIQEAQ"
     gene            19539..19820
                     /locus_tag="PLS35_01870"
     CDS             19539..19820
                     /locus_tag="PLS35_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNI17424.1"
                     /translation="MAFSSQSQVGADGALEQELATLIVEALRLEVLPETIDPSSALFG
                     EGLGLDSIDMLEIALLVSKRYGVELRSENKAALASLRALANHIAQHRIR"
     gene            19817..21238
                     /locus_tag="PLS35_01875"
     CDS             19817..21238
                     /locus_tag="PLS35_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNI17425.1"
                     /translation="MMMQSIDSQMAPASPARRTHQPRAAAGYPLVGHHAATSPLAWRR
                     DRAHSVHEFLCAAISLAAQLPRKHFAINLCRDRYHFAVGFAAALIARQISLLPTCRAA
                     EPLQQLARQYPGAYVLTDHDEGDIPIPRFSLLREMEQGVGPKDVPVIPADQIAAIAFT
                     SGSSGFPQAHAKSWGSLVMGARALGQQFGLTQSSSRVAVGTVPAQHMYGLETTIMLPL
                     QWGWSVHAGTSILPADLRTDLEQLESPAWLMTTPFHLKAFLRERTNLRGLEGIISATM
                     PLDRSIALSAERLWHVPVHEIYGCTEGGMLASRRTTAGEAWTLCHDLRMRHAEDTTWV
                     MGGHVGAWLQLSDRITLRSQREFLLLGPHRDLVKVAGKRASLTALNAILNRIPGVVDG
                     TFYWPDPGQTGTGRLRAFVVAPGMTRAAILAELRKRIDPVFLPRPLYVVDQLPRNTTG
                     KLPHEHLKTFARHIMHPQQHVHE"
     gene            21242..21562
                     /locus_tag="PLS35_01880"
     CDS             21242..21562
                     /locus_tag="PLS35_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17426.1"
                     /translation="MPTERPRQIRRDHPALAGHFPGNPLVPGVVLLTELLSILEQEAG
                     MKPGPLELSSVKFLRLLRPEEPFTLRWRFLPNQDIAFAVTRGEDMIATGTVHRARVTT
                     QVAS"
     gene            21559..22473
                     /locus_tag="PLS35_01885"
     CDS             21559..22473
                     /locus_tag="PLS35_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17427.1"
                     /translation="MSTGWRHQPERGSRRLMRLLVLITLWVGRPVARGLLYPICSYFL
                     LFSGGAQQTSRDYLRRILQRPPSWKDVFRHFHAFASTIHDRVYLLSGRHRYFDIHTEG
                     ASALHAVLAQNRGCILLGSHLGSFEVLRAQGIFDHKLPITVIMHEAAASNLNEVLHNL
                     RPEIRDRIIPPGTADTMLAVKERLDRGEIVGILGDRLFNDEKGMACEFLGKTRMFPEG
                     PFLLAALLQVPIVLFFGLYQGGRRYHLYFEPLADTIPVNGARRSETLRPWIERYVSRL
                     EHYCRVSPYNWFNFYEGPDHAQRSHPAV"
     gene            22430..23029
                     /locus_tag="PLS35_01890"
     CDS             22430..23029
                     /locus_tag="PLS35_01890"
                     /inference="COORDINATES: protein motif:HMM:NF039988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HNI17428.1"
                     /translation="MKVPITLSALILLCSLWAWLPASAEPAMGAEPFDVPGLMELLAK
                     VSSRQDRFTETKTIAMLTQPVVLKGTLAYVRPDRVEKHITSPYTEDLTVQGDRLTLSS
                     PKGTKQIAVDSHPLIRSFIEAIRASLAGELAVLRRLYHIKFQGTRQQWELTLRPLDAQ
                     AAEYVASIILRGEGDRLTTVDIRETGGDRSLMEIDGPVS"
     gene            23026..25347
                     /locus_tag="PLS35_01895"
     CDS             23026..25347
                     /locus_tag="PLS35_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNI17429.1"
                     /translation="MTLHARPALFLWMLLVFISGGIVANMTMTTDLTALLPRSADHRQ
                     ELLVSQLRDGVAARLILIGLSGAEGEALAQASRTIARKLRDTGLFASVNNGDPADFTI
                     VREVLMRHRYLLSAAVIPEHFTATALREALERQLQFLASPAGALMKTALPSDPTGEVP
                     HILAAFTPGEGPAMHQGVWFSRDGTRALLVAETSSPGFALDQQESAVQAVRTAFTSAG
                     LPEHAHLDMTGPGVFAVESRATIQRDSWRLSVIAITVVTILLFLVYRSVTPLLLSLLP
                     VLTGLLVGVAAVQTWFGFVHGITLGFGATLIGEAVDYPAYVFTQGIAGERLQQTLSRI
                     WPTLKLAVLTTVFGALSLLFSSITGLAQLGVLAFVGVLVAGFVTRWILPALPPGVIRS
                     APHQLLPMSWLPALASVNRIRWTIWVAALLALATLAMRHENIWDNDLANLSPIASPSK
                     TLDEQLRKDLGAPDVRYVLIVEGSSKEEVLQRSESAELPLRRLVDDGLLAGFDLPSLY
                     LPSDASQRKRQAALPSSDVLAASLAEAVKGLPFRAGLFEPFLHDVEMARTSPLIDIVN
                     LQQTPFFLKIRSLLVHTDDAWAGLVPLRHVTSPSGLADRLAREAGPGLTFLDLKSEAG
                     RLVNGYRHESLRLTALGVVAMTILLWWGLRDMALVSRVLLPPLLAILFVVTLLTVIGE
                     RLSLFHLVSLLLVLGIGLNYALFFNRPFSDEEEKRRTWLSLTVCMLATLSAFGALAFS
                     RTPVLHAIGLTVGLGAAGSLLIAAILGQQRVSQ"
     gene            25344..26534
                     /locus_tag="PLS35_01900"
     CDS             25344..26534
                     /locus_tag="PLS35_01900"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNI17430.1"
                     /translation="MMAPLTVTAMATANPLGLGLAETWQALRTERQGLRPNDFEDADI
                     KTWIGRIEGLETEPITGELADFDCRNNRLALVGLRQDGFEQAVASARQRYGPDRIGVL
                     IGTTTSGILETEWAYRRRAAGGELLPLTVRYRHTQNLFSVSDVVRRHLKLTGPVASIS
                     TACSSSAKVFASASRWMAAGLCDAVVVGGVDSLCFMTLYGFASLGLLSPRPCRPCDEH
                     RDGLSIGEAAGFLLLERETTRDSGLRFLGYGESSDAHHMSTPHPEGLGAVLAMERALD
                     RAELKPIDIDYINLHGTGTPANDLAEDRAIWKTFGERTPCSSTKERTGHTLGAAGITE
                     AILSLLCLRHEFIPGMVRIEQPDPQLRSGIMLRSESRPLRTVISNSFGFGGSNCSLIF
                     GKRP"
     gene            26531..27328
                     /locus_tag="PLS35_01905"
     CDS             26531..27328
                     /locus_tag="PLS35_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HNI17431.1"
                     /translation="MMEFWVISIGIAGPGLDGWAAARPILAGETEYRPAPLTPPNLKL
                     LSAAERRRCSQSARLAIAAAQDALLAGPLQGEAIATVFTSSDGDGAITQEICESLAGA
                     AREVSPTSFHNSVYNAPAGYWSIATRSQLSSTSVCAYDVSFAAGLLEAATYTTVERCP
                     VMLVASDLPFPRPLYGIRPVEESFAAALLMTPTSHPDACMRCEIALAPHQAITAFPAT
                     IPESLRSNPAARSLPLLALLARQAADTVRLEYTDESDLVIDCKPEGH"
     gene            27328..27804
                     /locus_tag="PLS35_01910"
     CDS             27328..27804
                     /locus_tag="PLS35_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17432.1"
                     /translation="MERRCDDIAELVPHRGTMCLLDSVETWDEHHIVCRATSHLRPDN
                     PLRDGSGLRTLCAIEYGAQAIAAHATLLGSGHRQRAAEGVLASLRHVTTTQSHLDGLK
                     GALTILAMLQLSHQQGRIYDITVTADGQTLLTGRLSVMLEADHSGEARSVSQEGAA"
     gene            27801..28523
                     /gene="fabG"
                     /locus_tag="PLS35_01915"
     CDS             27801..28523
                     /gene="fabG"
                     /locus_tag="PLS35_01915"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNI17433.1"
                     /translation="MSKRALVTGGSGGIGQAVCQVLGEAGFEVIVHGCTRMDRAEAVA
                     QAIRDKGGKAEALCFDVTDAAQTRQALDRLQPAGPIQVLVNNAGIYDDAPMAGMTNDQ
                     WHRVLDVSLNGFFHVTQPLLLPMIRTRWGRIVTITSVSGLIGNRGQVNYAAAKAGLHG
                     ATKALALELASRHITVNAVAPGIISTPASLDTFGAEAIERLVPMKRAGTVEEVAGLVS
                     FLVSDTAAYVSGQIISINGAQA"
     gene            28520..>29067
                     /locus_tag="PLS35_01920"
     CDS             28520..>29067
                     /locus_tag="PLS35_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI17434.1"
                     /translation="MRERDKDAIAIIPAYNEAGTIHHVASRTLHYVERVVVVDDGSTD
                     QTVSSLRELPVTVLRHANNLGKAAALRRGMQHAVAQGATAIITLDGDGQHDPAAIPAL
                     LAMHRRDPRAIVVAARRRHRHTIPRLRVLAQRIADFWISLAAGCTIEDSQSGFRLYPI
                     SAWTAAGWPCDAASGFVFESEIL"
     assembly_gap    29068..29167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29168..30175
                     /locus_tag="PLS35_01925"
     CDS             <29168..30175
                     /locus_tag="PLS35_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI17435.1"
                     /translation="VVGDFRLSLSVSARVAAVPYLTVTNAHWSPFAHQHFVVPELAIT
                     ERFGPRLGQALFTMMRPFVFAQQGHALNQVRRDYGLPALPYSLPHQFTDADVTLYADV
                     PELVPTYHRPPHHHYLGPVLWSPDSVPRWWDSLPDTGRMAYVTLGTSGRADLLPLILD
                     ALEQLGIEALVSTAGRSIPGRLPPGTRVAQYLPGQAAAARADLVICNGGSATVYQALA
                     AGKPVLGIPNNLDQYLMMDYVRRSGAGDYLRAGTISRAAVTMLCDGLLGNPAFRDRAG
                     RVQTAIEQNRTARAFDDHLRSVLGTGRSHQAPSKIRTPKVSAFDSVPEESSPSNRASR
                     R"
     gene            30172..>30591
                     /locus_tag="PLS35_01930"
     CDS             30172..>30591
                     /locus_tag="PLS35_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNI17436.1"
                     /translation="MNQTCTVHDPIPPQDAAQMEQRVEEHLTVHSRALTGLFLLAVLY
                     TLHFGQVIFLPMTIAFLLAVLFAPLLRRLRQWHLPEPLGAAILLALLVSGLVYGGSRL
                     AEPAAEWSTKVPVALRSVEYKIQPVKKSMQDVTKATEL"
     assembly_gap    30592..30691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30692..31069
                     /locus_tag="PLS35_01935"
     CDS             <30692..31069
                     /locus_tag="PLS35_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNI17437.1"
                     /translation="LLGMPNPILWGVMAGLLTFIPYIGHIFGIVVVMLVAALTFDDLG
                     QMFLIGGVYWGLAILEGSFASPMILGRRLESNPVVLVLAIMIWGWIWGVGGALLAVPL
                     LAAFKIMCDHLEPLQPIGEFLGK"
     gene            31169..32083
                     /locus_tag="PLS35_01940"
     CDS             31169..32083
                     /locus_tag="PLS35_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17438.1"
                     /translation="MNRRWIVALAFALCTIPSLVFAESPPQSVHWGAIAFPDHDPTLT
                     LSTAVLDRFTQFDGAGRRYNDINQTMGFNFFTISWTKPLTQLPGWNLNLTAGGGPTRD
                     GPSRFLQNDVVHKLRGFDPVPVGEKRDANDFMLSGTMTRWFGLLGTSEVFFAGVGGAG
                     GSLYYEPYVQAGFRRLAILNLIPVLGDYLRVSGLARYGRPFSGAAFHQVAPQSYLAQA
                     SVGLGNYHHWKDCTPWEIELAVTVDSGLFVDHRGDALEERFVSLALRYSAFTFETWND
                     LINQKDYGPTFGARLTLDLFYMYDRWFH"
     gene            complement(32111..32494)
                     /locus_tag="PLS35_01945"
     CDS             complement(32111..32494)
                     /locus_tag="PLS35_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098609 - cell-cell adhesion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin domain-containing protein"
                     /protein_id="HNI17439.1"
                     /translation="MRRQHIFAVAAVLALTGLPAFAADLEVQVVDKNCFIEIFEDDNF
                     DVDDPHVVLQGPKEYATLKNLAGKDWSNDIESVIVGSNATVRAYEDKDFKGTEIAFAP
                     GQRVPNLGKLDMANDIESLKITCGK"
     gene            complement(32661..33089)
                     /locus_tag="PLS35_01950"
     CDS             complement(32661..33089)
                     /locus_tag="PLS35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNI17440.1"
                     /translation="MKSSSSIHNLACALCVAAAGLMVGACNTTKATVDTTVNFFSSTS
                     PNDLFTSDGMVVKEQKINLFAGVAYENLRQEAATGSGQYVTALAALYEIPVSKQQEFG
                     QVLQRNHADLFAADLQDDHSAHLKMVSALNRELTAAALLP"
     gene            complement(33322..33651)
                     /locus_tag="PLS35_01955"
     CDS             complement(33322..33651)
                     /locus_tag="PLS35_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNI17441.1"
                     /translation="MHTILLLTISNIFMTFAWYGHLKYKESPLWIAILASWGIAFVEY
                     CFQVPANRIGHYEFTAAQLKTIQEVITLMVFCVFSAVYLKEELKWNYLVGFGMMIGAV
                     FVVFKEW"
     gene            complement(33713..34270)
                     /locus_tag="PLS35_01960"
     CDS             complement(33713..34270)
                     /locus_tag="PLS35_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17442.1"
                     /translation="MPLLIRILIVGFLGLLNGCYVGEGTGYLRFEGKVSNEEGQPLDG
                     REVRIQLSPVESIHVRNVGKVAHTQNDGKFVIETHGIVGVVYVAPIGWIVVPLGVVIA
                     ALTPWTNSEVQDFLRPSYTFGPDCLDHPKIIALKIDSADYGMTFIEKDIVRKLYHGVE
                     RKNGESCARVYDFGELEISMGSLSR"
     gene            complement(34777..37284)
                     /gene="uvrA"
                     /locus_tag="PLS35_01965"
     CDS             complement(34777..37284)
                     /gene="uvrA"
                     /locus_tag="PLS35_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNI17443.1"
                     /translation="MSNSIIIKGAREHNLKNLDVEIPRDKLVVMTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLEQMGKPDVDSIEGLSPAISIEQKSTSHNPRSTVGTV
                     TEIYDYLRLLYARVGHPFCFQCGQEITAQTVQQMVDAICELPEGSKFQILSPIVRGRK
                     GEYRKELLEMRKAGYVRARINGEMVDLGEDITLDKQKKHTIEIVVDRLVMKPGDALMR
                     RIADSVETSVKLAGGLVGVLSETGKLHLYSDKLACIKCGVSYPEITPRVFSFNSPHGA
                     CPACDGIGYAMTPGATEEDDFTLLERCETCQGARLKPESLAVKIAKKSIAEITGLSVR
                     AAADFFATLKFTERELVIAHRILKEIRERLGFLVNVGLDYLTLDRPAATLSGGEGQRI
                     RLATQIGSGLVGVLYILDEPSIGLHQRDNRRLLQTLLRLRDLGNTVIVVEHDAETMEA
                     ADYILDLGPGAGTHGGRIIAQGTPKQVKANPDSLTGLYLRGAQMVSLPQRRRTPKGFV
                     TVVGAKKHNLKGVNVNVPLGLLTCVTGVSGSGKSTLVLEVLFHSLSQLLYHKKPKIDG
                     CKEIKGVDALDKIIDIDQSPIGRTPRSNPATYTGLFTFIRDLFSNLPESRVRGYKPGR
                     YSFNVKGGRCEACQGDGLIKIEMHFLPDVYVTCEVCKGQRYNRETLDIQYKGRSIADV
                     LNMTVDDALEFFENIPFIKSKLQTLHDVGLHYVKLGQSATTLSGGEAQRVKLSRELSK
                     RPTGRTMYILDEPTTGLHFADIQRLLDVLDRLVETGNTVLVIEHNLDMIRNADWIIDL
                     GPEGGDRGGEIVAEGPPKEIAKVKRSYTGQVLREAGV"
     gene            37487..>37678
                     /locus_tag="PLS35_01970"
     CDS             37487..>37678
                     /locus_tag="PLS35_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17444.1"
                     /translation="MHDVLSLFKRNINQLFGITVDILNVGRSLQQLSLSMQILANNGV
                     VQAAKIPGGKGRPMLALVEI"
     assembly_gap    37679..37778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37779..38433
                     /locus_tag="PLS35_01975"
     CDS             <37779..38433
                     /locus_tag="PLS35_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17445.1"
                     /translation="AAKIPGGKGRPMLALVEILNNTPKEIRPEVEALEHLCAGLAKVT
                     ASSSNIVWRYHQLIASLLSAMTQGELSSKSKSLETLSHLRFTTAADVTQLTQHPAFTA
                     VGGLERENRLYLVHHCQANLTELQERLKEALRCLGETHQALNGLKMIGLTARYMAFCI
                     SSEAAGLAEAEANFKNLAAEISRVVDDLDNKTRAMKDAIEHGQELLGLLLKGHTYAK"
     gene            38423..39202
                     /locus_tag="PLS35_01980"
     CDS             38423..39202
                     /locus_tag="PLS35_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI17446.1"
                     /translation="MQSKVLFNKDDHQWVVIGRDPEKDDHVIDTNEYVIIHRGHAMLL
                     DPGGIQIFPQVLAELAKYVRMQDIKVIFASHQDPDICSSLAMWLDLNPAIKTYCSWLW
                     TGFVSHFSTGAVITLNPIPDDGMRIKIGEEGPEMEAVPAHYCHSSGNFSLYDPSAGIL
                     FSGDIGGALVPDHHASLLVTDFEQHIQYMRGFHLRWMPSTTALRGWVQRVRAIKPKMI
                     CPQHGSIFEGENVGKLLDWLESLEVGQWKGSAPKSETRAAA"
     gene            39296..40063
                     /locus_tag="PLS35_01985"
     CDS             39296..40063
                     /locus_tag="PLS35_01985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17447.1"
                     /translation="MVRAEQSPITEPAAKAVSTWSGQAREQAVQRMFTAIAGVYDLNN
                     TLLSFGLHHHWKRLTASYVPTVTNGRALDIGAGTADLALLIEPKMGEQGHVVASDLNH
                     AMLAEGLRKVSGRGLLDRITCLQANAEQLGFPDNTFHAVTTGFCMRNVGNLTQAFTEI
                     RRVLRPGGRFVCLEFSRPAYGWLRALYDWYSFRLLPWIGTRVARDRTGVYEYLPASIR
                     TFPDQERLATLLREAGFREVEYRNLTGGIVAIHVATK"
     gene            40130..41773
                     /locus_tag="PLS35_01990"
     CDS             40130..41773
                     /locus_tag="PLS35_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI17448.1"
                     /translation="MNSILYIFLPCKKVYPIGVTYLADFIHRRKPEVRQRILDLSLFS
                     ESQRTQAIRDAALEFKPDLVCFSWRDIQIFSPHEGDASLEHAFNFYFASNPIKRVAAS
                     FAGLKQLYRYYSHIRANLSYPWLIRKEFPKTQIMIGGGAFTAFADQLIEKLPEGTIGI
                     LGEGEDAILKVVNGESLENERYIIREGKQTRKGNQGSPALLDALTVDLPYLTSIFPQH
                     ASYMDESIGVQTKRGCPYDCAFCLYPYIEGKRVRYRPPEMVVKDISQHYHQWGARRFW
                     FTDAQFITGKEAYPQCTEILERILSEKLEIEWSGYIRTSLITPELAKLMVRSGVGDLE
                     VAITSGSQEVLNNLHMGFKLERLYDGCRYLAEAGFKGKVILNYSLNSPKETEESLLQS
                     VESYKKVASILGEERVFPLMFFLGIQPNTDLEHRLLEEGYLSAGYNPLMLTPTSIRKL
                     LYNPAPLNKIIAKACLTAWEQKEGSRDPRTWTGSLSQTPPAGAKPDQAHYADANLIRG
                     IQNNSGREALLTLEEILRSRRPAQPTAAATEKTAVSPTS"
     gene            41939..>42054
                     /locus_tag="PLS35_01995"
     CDS             41939..>42054
                     /locus_tag="PLS35_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI17449.1"
                     /translation="MYKTIYIPVDNSDHSNTAVDVGVELAKTYGSKIVGSHV"
BASE COUNT         8419 a        12907 c        11688 g         8740 t
ORIGIN      
        1 gccaatatcg ccgtgctggg cgccgatgga accattctcg ccgtcaatga agcctcggag
       61 cggttcgcca cagccaacgg gttcaccgag aacgcccacg cggggatccg acacgttccg
      121 gacaggacat tcggatgttc tcgcgcgaga gtccgaacgc ctcagctcgt ttctgcggcg
      181 atggtttcgt agcaatcgtc acccctatgc aggggcagta ggactatggc gcggtggtca
      241 agcatatcaa tgtgtcccac ttcaagcagg agtaagccgc catgcatcgc agccaagcag
      301 ggttgatggc catcctagag gcggcggtgg acggcatcgt cgtgatcaac gaacggggca
      361 tcatcgagtc tgtcaacccc gccgtcacga aactgttcgg ctacactccg gaccggttgc
      421 tcggtcaaaa cgtcaaaatg ctcatgcccg atccctacca caccgagcat gaccaatacc
      481 tgcgcaatta cctgacatcc ggcctgaaga aagtcatcgg catcggtcgt gaagtgctgg
      541 gacggcgaag cgatggaacg tcgtttccga ttcatttagc cgtcaatgaa atactggtcg
      601 acgggtcgag aaagtttgca ggaatcgtcc gggatatctc gtctcagatc ctgtccgaaa
      661 tgcagttccg gcaggtggtc gactcggccc ccaacggcct gctgatgatt gatcctcacg
      721 gcactatcat gatggtcaac aagcaggtcg aagccatgtt cggatacagc cgcgaggaac
      781 tattgggaaa ccgagtggaa atgttgatgc cggaacggtt ccgatcaaat cattccacct
      841 accgtcgcgg ttatttttcg gggcctacct cccgcgccat gggagtgggg cgggatcttc
      901 ccggactgcg tcgagatggc tcagagttcc cggttgaaat agggctcaac ccgattgaca
      961 cgccccaagg ccgacttgct ctcgcgtcca tcgtcgacat ttcaggacgc aaacggatgg
     1021 aagatgcgtt agccaaagcc gccgaggacc ttgaagaaaa aaaccgcgaa ttgtcggaaa
     1081 cgcgcgacat ggcgctgcga gccggccagg ccaagacgga ttttctcgcg accatgagtc
     1141 acgagatcag gactccgatg aatggcgtgt taggtatgac aaccctgctg ctcgacacca
     1201 ccttgactgc agagcagcgg gattacttgc agacgctcag aagttccagc gagtccctcc
     1261 tgcgcatcat caatgacatt ctggacttct caaagatcga agccggcaag ttcaccatcg
     1321 aacatattcc gttcgatctc cggctcacaa tagaagatac gctggacatt ctggcgccca
     1381 ccgcgcaagg acggcagctc gaactggtgg gtttgattga tgcgcaaact ccccgcacgg
     1441 tcgttggtga tcctggccgc atccgtcaaa ttctcaccaa cctcgtcggc aacgcaatca
     1501 agtttacgga gcatggtgaa gtcctgatcc aggttttaca gacagacgaa gatccaggct
     1561 cagtcgtcct ccgatttgaa gtgatcgata ccggcatcgg cctgagccct gaggcgcaag
     1621 ccaaaatgtt tcaggccttt actcaagccg atagctcaac ggccagaaaa tatggcggaa
     1681 cgggtcttgg gctgacgatt tgcaaacgtc tcgtcgagtt gatgggcgga acaatcggcc
     1741 tgcagtcatt cgccgggcag ggaacctgcg tttggtttac aatccgcctt ggcacccaca
     1801 cggacgcggc tccggcggtt cctcccatcc cctcaggcct ggacctcacc ggcctgcggg
     1861 tctgcctggt cgatgataac gcgaccaacc ggagcctcct ccagtaccat gtgtcggcgt
     1921 ggaacatgca ccacgaaagc gcggtcgacg gcccgtctgc attggccctc ctgcggagag
     1981 gggtggaaga agggacgccg ttcgatgtcg ctattatcga tatgtacatg ccggagatga
     2041 acggcctcga gctctgccgg ttgatcaaac aagatccggc tcttcgaaaa acgcacctaa
     2101 tcatgctcac cgcttcgggc caacgtggag acagcctggc tgcccaggag gccggcgccg
     2161 ccgcatacct gacgaaacca atccgcgaac gccacctcgc cgattgcatc cgtctcatct
     2221 tcggccgcga caatgcgaag gaacagccgg ctccgtttct tacgcgtcat accatcacgg
     2281 aagccatagc tcgcgaggcg ccacggctgc tggtcgtgga cgataacccg gtcaatcaga
     2341 aggtcgccgt gaagatgctc gaaaaattgg gatgccgcgt ggatttggcg ggaaacggga
     2401 tggaggcact ggctgccatc tgccgacatc agtacccgtt ggtctttatg gactgtcaga
     2461 tgccggatct cgacggcctc gagaccaccc gattgctgcg gtcccaggaa aagccgggcg
     2521 aacatcttcc ggtcatcgcg atgacagcca atgcgatgga gggcgatcgc gaaagctgtc
     2581 ttaaggccgg catggatgac tttatcagca aaccgattat cacctccgat ctgaagaaga
     2641 tcctcgcgcg atgggttccg cagaaccaat tggaaggtga gcaagccgcc tcgcctgaaa
     2701 ccccgtcgtc ttgattgccc cgttccccca agacgctcct catccagccg gcgcgcccga
     2761 cttcatacaa gcttcgcgtc ctctctccct atctcgcgat tgacagaagc cggcgcgccc
     2821 actaggatac gccctcaaca aggagggtac gtgcacgcgt gagtcatccg tctccatccc
     2881 atactccatc cgcaccatct acagcagccc atcgcgtcga gcagctcttc gaaatcctga
     2941 tcttcgggag ccgatggatt caagctcccc tctatggcgg gctgatcgtc gctgaactcc
     3001 tctatgccgc caagttcctg acggagctct ggcacatgat cgtccatttc cgcgaggaga
     3061 gcgaaaccct gttcatgctg ggcgtgctgt cactgatcga cgtcaccatg gtcgccaacc
     3121 tcttgaccat ggtcatcatc ggcggctacg cgaccttcgt cagcaaactc gatctggaaa
     3181 cccatccgga ccgccctgaa tggctcaccc atgtggaccc cggcaccatc aagatcaagc
     3241 tggccgcgtc gttggtcggt atttccagta ttcacctcct caaggctttt gtggacgtga
     3301 ccaacgaaaa ccccgaacac atcaagtgga agatctttat tcacatgacc ttcctcggtt
     3361 cggcgatctt tctggcctat accgacaagc tgatgcagcg ggaccggaaa cactaaccgc
     3421 acctctggaa aggttggggc atcctgttcc gtcgtatcaa ttgagatacg ggtggtatct
     3481 attccgataa tcggtcaccc atgtcgtcag acactgtccc ttgtatccgc cgcctctccc
     3541 cagccattaa cgaccctgca ctgtcgcttc gaatgaccgc cttgtccttc tccaccgagg
     3601 catgtgttgt gccttttcat cccgtgaagg aatcacactc gccatctggc ggccggtcat
     3661 ccaccatcgc cacggagtcc cgatgactac tcccggtcct gagaaagata cctatctgac
     3721 cgttgccaac attctgggag aggaaacgga actgcttcac ggccccttct cggacgacca
     3781 ccctctgaag gtcctggaac gggcagctcc tacggaagcg tcggacaagc ggtatctcga
     3841 cgaactcaac cgccacatcc acgcctgcca tccgaagcgt gccggcctct gcctgtccgg
     3901 cggaggtatt cgaagcgcaa ccttcggact gggcgtcctg caatccctgg cacgcctgca
     3961 gttgttggac aaattcgact ttctctctac ggtgtccggc ggcggctata tcggcagctg
     4021 gcttaccgcc tggatccatc gccatccccg gggcctaaaa ggcgtcatcg aggatctcaa
     4081 cagaccatcc aaggccggtt cggaggaagc gtctctgccg gtccggtggc tgcgaaacta
     4141 tagtaattac ctcagtccgc acttgggctt cttctccgcc gactcctgga cattgctcgg
     4201 catctatttg cgcaaccttc atctgaactg gatggtcctg cttcccttgc tcatgatgcc
     4261 gctgctcgcc cctcgctgga tcatcgctct cgcccagctc aatgccaaag agacatcacc
     4321 gtcgcccatt tcagccgcct cgctgctggt catcgggata ggcctggcgg tcatggcgtt
     4381 gatctatttg cacctttgca ggcccaccct tcgtgagttc cgtcgcgaca cctggtggca
     4441 aacactggag agccagcgct ggtttctgat tgcctgtttg tgtcccttgc tcatctccgt
     4501 cacctgtctc accaccgcat gggcctggtt tcgcaatggc ggtggtggag ccgatcaatt
     4561 cacctgggct cacgcggtct tcggcggggc cttgatccac acagtcagct ggctcttttc
     4621 cgccgtcgcc ctcaatcgat tcagggcatt cacccgctgg ctcttgttag aaaccctcgc
     4681 cgtggccttc accggcgcgc tgggcggatg gcttctgcgg gcatccttca tcaaaacctg
     4741 cggtcaatgg aacgtcgcct cccatgcgga atggtttgcc acactggcag tccccggggt
     4801 cctgacgata tttctcctca ccgcgacgat ttttgtcggc ctcgccagcc ggttcactga
     4861 agaacaggac cgcgaatggt ggggccgcac cggttcctgg gtgctgatcg caatggtcat
     4921 gtggtcaggc ctctgcgcca tcgtgatctt cggacccggc ctgatccact ggatgacgcc
     4981 acaggtagct gcctcggtcg gcggactgtc cggccttctg acgttatttc tgggattcag
     5041 ctctcggacg accgctcagc aacaagagga acggtcaacc gtgaccgcag tcaccgacct
     5101 ggcggtgcgc gccgccgccc cgctgttcat ggtctgcgta ctgatcgccc tcgccctggg
     5161 caccagctgg caattggatc tgctcgcgca tcactaccac atgcacctga accatcttga
     5221 cggtcccatg cagaccggct tgggaatcgg aagcgatccg tggggtcacg atcaaattct
     5281 gcacaatact ccgtggtggc tgctgttccg cttcacggcg accgtagtgg ccctcgggct
     5341 gctgatgtcc gtctttatca acatcaatcg attctcgctt cacgcgatgt atcgcaaccg
     5401 gctcattcga gcctatctcg gggcgtcccg ccggcaggca gaacgtgaac gaacgtttaa
     5461 tcccttcacc ggattcgaca atctcgacaa tccgtccgta agcgacctgc gttttgatga
     5521 tgtccgcctc gctcggtggt tctctgcgca ggagcagaaa caaccgacta tgcaggcact
     5581 gctcagttac ttacagttat caaatccgac ggacgagcaa caggaagcct tgtgcgcgca
     5641 actcccgcag gatctgcgac agactcaggc atgcaagcgc ctcagcgagg ccgcacacca
     5701 actccgtctc ggtccagcgt gcgtcgcgaa gctgaccgcc ttacagatgc cggacactgc
     5761 cgccggtcgc gaggcctcca agtggttcag cgatctcttc atacacaaac ttcacgtaca
     5821 aagtcctggc ccgttccatg tgatcaacat tgccctcaac ctggtcaagg gcgataattt
     5881 ggcctggcaa caacggaaag ctcaatcatt tacgattagt cctctccaca gcggcagttg
     5941 gaacctgggc tatcgtcgct ctgaagaata cggagccaat cgctacatcg gccaacctct
     6001 gtcgctcggt acggccttgg ccatttcagg ggccgccgcc agtcccaaca tgggatacca
     6061 ttcgtcctcg gcggtgacgt ttcttctggc cctcttcaat atccgcctcg gctggtggct
     6121 ggggaacccc ggccgcgcag gcgcggacac ctaccaccaa gcctctccga ccctgtcgat
     6181 tcgtcccctt ctggctgaag cattcggcct cactgactcc tgccacccct atgtctatct
     6241 ctcggacggc ggacatttcg agaacttggg gttgtatgaa atggtcttgc gtcggtgcca
     6301 ttgcatcctc gtggtggatg ccgggtgtga tcctcgtctc accttcgagg accttggaaa
     6361 tgccattcgc aagattagaa tcgatctcgg gatcgacatt gaactaaacc tggaccagat
     6421 caagcgggcg gagggcgcca cgacgagcaa tcgccaccat gccatcggca ccattcgcta
     6481 tgacaaggtg gatgacaatg ccacggccgg gacattgatc tatctcaaac cgtcattgac
     6541 gggcaacgaa ccatctgacg tgcaggatta cgcggcccgc cattccgcat ttccccacga
     6601 gccgacctcc gatcaattct tcgatgaggc ccagttcgag tcctatcgcc gtctgggtga
     6661 gcatatcgcg caagaggtcc tcaggccggc gctccatcgc accgagcagg acttttcacg
     6721 actgtgccat gcgttgcgtt cgcactgggt gacgaccccg ccgggaatgc aggagtcttt
     6781 cctcggcgag acggatgatc tcaaagacct cgagcggctc ttgcgggacg atccggacct
     6841 gttgcgctac gacatggaga tctatcccga gctctgttcc gtcttcggac tggattgcgg
     6901 caacgtcgcc ctcaatcctc gcgccgcgct ccatacctgc aacctgcaaa ttcagctcat
     6961 ggaacaggtc tatctcgcgg tgaagctgga agagttccac ggccatgcgc tcaatcgcgg
     7021 atggatgaac ctgtttcggc gttggacttc ctcggctacg ttccgaatgt tctggcccac
     7081 cgtgtgcagc atgtacagcc aacccttcgt tcgttttgcg gggcagcatt tcaatctttc
     7141 aatcgaccag gtcgcggtgc tgcagccact ggagcagacc ggtgacctta gctcgctgtt
     7201 ccgggagtta ttgatcgaat gggcctcggt acccgattat gcggagacat tcatgaatgc
     7261 cctgaagcgg ccgctgccgc tggaggaagt caccgatggc ccgccaccgc cgctggcggc
     7321 ctggcagatg cgtttgccgg ccatcgactc ccaggccgga ccagtcggtt ccggtgaaat
     7381 tctggccgtc gtcgccgtga ctcggctttc cgtgaccgga caccggctcg ccttatatgg
     7441 ttgggttcgt cctgcgtacc ggggattggg tctggggcat cgtctgttcg ggaaggccat
     7501 tgatgtgctg acggaggtat attccggcca taatctgatc gtcgatttag gcccggacaa
     7561 tgccgcctgg gccggcacgt ccatacgcaa tgtcggatgg ctccggttct atgaacagtt
     7621 gggatttaca cgggatcgtt cggatcccaa gcgctttcag atgatgcggg agctttcgta
     7681 ggaccgggaa aagaaccagg gcaggtaccg ttcagaccga ggggagttgc acggacgaga
     7741 gcagcgtctg aacctgtcga agtagcaatt cacgactgaa cggtttttcc aacacgccat
     7801 gggctccgaa cgccatcgca atcttgagaa tgtcgcatcc ttgcaggggc tgaccggata
     7861 tgcccaggat ttttacagac gattggacat tgtgtagcat caggatggtt tccagcccat
     7921 cctgcaccgg caaatacata tccagaatca tcagatcagg cgcgtctcgc tcaatggtcg
     7981 ccatcgcctc ccggccgtct gatgcctgga tgacacgata accttccgcc tcgaggacag
     8041 cccgtaacca cccgcaaatc tgctggttgt catccgcgat caaaatcaac ggcattcagg
     8101 gccctccttg ctgtcacacg tagtggccag aagacatgga ccggcttggc agagctctcg
     8161 cgtacagtgt cgcggcctgc gttcgcctgc tgattcccag tttggaaaaa atattcgcga
     8221 ggtagttttt gacggtttta tcggagagcg cgagggcgga agcgatttcc ttgttcgttt
     8281 tgccttcggc gaccaggggc aacaaccggt gttcctgcgg agaaagggca gccaacgact
     8341 tggaggaaag gccatttccc atgtcgtgca cttccctcag caccataccg agcaagtgcg
     8401 gctggaacaa cacatcaccg gaggccactc tgcgaacggc atccagccag gtgtcaacgg
     8461 gcgcatcttt gagcagatag cctctcgctc cggcgacgat ggcctcctga accacgacgg
     8521 gatcgtcctg aatactcagc atgagcaccc gctgtgcggg attgcgagcg accatcgtcc
     8581 ggcaagcctc tacgcctgag agatccggca gacggctatc cagtaacacc aggtcaggct
     8641 tgacctgttc caccagctcc atcccttcca cagccgacat cgcttctccc accacgcgaa
     8701 aatccgtcgc tgtcagaagt tgttcccgaa tcccgagacg caccatccga ctatcatcga
     8761 tcaatacaat gcgatagaca gactgttgct ccatgtctcc cccattgcga taacactgag
     8821 gcctcgccgg ggtactagtt tcggtacatt cgcgtagtag gaatataggc aggaggtgga
     8881 tttacgcgtc agcattcggc tgacgcgacc acaacggaca ggcgaggctc taaggagaaa
     8941 gactaagacc taatcaatca atcgcgcttc gatggcatat cggatcagtt caccggtact
     9001 ttcgcacgac agtttttcca ggattctcgc acgatacgta ctaatggttt tcacgctgag
     9061 gcacagttct tccccgatca tcgaaaccga tttgccctgt cccagcaacc ggaggacttg
     9121 cagctcacga tcggagagcc gcgcatggag ttccccctcc gtcccggttt caaaggcctg
     9181 cgccagctgt tcggcaagag cggcggtaat ataccggccg ccttgcaaca cccggctgac
     9241 cgccgccagc agctcggagg gcgcgctttg cttggtgaga tacccggagg cgccggccct
     9301 gattgcccgc attgcgaatt ctttttccgg atagaggctc aacatcagca cgggcaagcg
     9361 gggttggagc aacttggcct ccttcaagac atccagcccg tgtttatcgg gcagcgcgat
     9421 gtcgagaatc atcagatccc aaggctgacg ccgtacggcg tcgagcgcct cctctccggt
     9481 ttcagcctcc aaaatttctg agcacagctg ctcgtcctcg agtaaatcac gcacacctcg
     9541 acggacgacc ggatggtcat cggctatcag acatcgacat ttattcatca actgttttcc
     9601 tccgccgccg acgtgatgcc gtcgtttcat gttgtgtttc acacgctggc tccggctccg
     9661 aactcaccgg aatcaccaca tgcacccgtg ttcccgtctc cgaactggaa aggatgtcca
     9721 cctgtccgcc taatatttcc accctttccc gtatacctcg caagccgaat cccccggtgg
     9781 gagccagtcc cgccacatcg aaaccgatgc cgtcatcctg gaccgtcatc tgccaattct
     9841 gttggccgct cgtaatggcg atggccacag tcgtcgcgcg cgcatggcgt atgacattgg
     9901 tcagcaattc ttgcacaatg cggtagaacg cggtttccag cgtgggacag cgtgacggtc
     9961 ccccctcccc ttgctcgaac accagcgaac agcgcagccg ggtgcgcgcc tgcacgtcgg
    10021 caatcagggc ctccaatgcg gccttcagtc ctaactcttc caacaccggt ggccggaggg
    10081 cccgtacgat ctgacgcagg cggacaaaga gcagatccgt agtttctaac gcgcgggcca
    10141 atcgttcctg gcttcgaggg cccaaggctt tggaacgcga ggcgacactc cgcttgacgg
    10201 aggccagatc gaacttgagt gaggtcaaca actgccccag ttcgtcatgc aactcacgag
    10261 agagacggcg acgttcattg gattcgacca tttgcagttc ctgcgacatg gcctgaaaac
    10321 gtcgataggc ttcctcgagc gatgcatagg cgcgcttggg acctgtcaca tcgatcatgg
    10381 cgccgatcat gcgtatggcc attcccacct catttcgcac gatgtgggcc cgatccagaa
    10441 aatagccata ggtcccgtcg gccagcagga accggtactc tgcggcgagc gtctgcacct
    10501 ccgactcaat ggcctgttgg accatggcca gaatcggtgc cttatcgtcg ggatgtaacc
    10561 gcatcgtcca ggcatcaatg ctcggttccc gctggggatc gtacccgaac ttctcccggg
    10621 cattggggct ccaccaatgc gcgttcgtca caagatccca gtcccataac acgtcgttgg
    10681 tggcctgagc cacgagacga aaccgttcct cgctggatcg aagccgaagc tccgtgcgct
    10741 gccgctccga catgtcccga accgtggcga agttatactc gcgtccttcg aagaccacga
    10801 aattcgccac gacctccacg ggatagatct cgcccgattt ggatcgatgt tgcgtttcca
    10861 ggcgctgacg gccggtctct cgaagctcct gccagtggcg gacccaggct tccatctgat
    10921 aattgggatc gatatccggc acggccatgt gcagtaattc gtctttcgtg tagcccgtcc
    10981 gttcgcatgc cgcggcattc acgagcaaca ggcgcgctgc ctcgtccacc caaaacacca
    11041 tgtcagccgc gcgttccatc gcgaattgcg tcagatgcaa ctgcgcctcc gcccgcttcc
    11101 gattcgtgat atcccgcatg aacgcgcaat aatattcccg gccttcgtgg accagaaaat
    11161 tgatggtggt gtcggtctgg atcaccgttc catctttttt ctgatgccgg gattcgtacg
    11221 agaaggactt cctgactttc aaatgctgcc agcgggctcg ccagttttcg cgggtgaaat
    11281 tcgtgtcgat atcatgcacg gtcttggata aaagttcctc acgggtatag cccaacatgg
    11341 cacaggctgc ttcgttgaca tccagaatcc gtgcctccgc actgaccaga aaaaccgcgt
    11401 ccaccgcctg ctgaatggag aactgcatga ggcgcagggc gctctccgtt cgcttccgtt
    11461 cgatcgcgag gcaggccaga tgcgaggccc gggcgacaat cggcgcttca gcggtcggct
    11521 gacggggtgt ctgattgtag atggcaaagg tgccgaggat ttttcctgct gccgagatga
    11581 tgggggtcga ccaacaggct cgaagcccat gcgccagggc gagctcacga aagtccgccc
    11641 agaagggatg gctcgcaata tccgtcacca ccacttgctg tccggtgaat gcagccgtgc
    11701 cgcatgaacc gcgcgcagga ccaatggcca tcccgtccaa aattcgagca taggacgccg
    11761 gaaggctggg accggcgcca tggcgcaagt gggcttcatc ctcctccagc agcaacacgg
    11821 agcagaggac gccgctggac agtccctcga tcatcaggca aatttctgcc agcactgccg
    11881 acagtggagc atccgatccc atcatctcca agatgcgggt ttccgacgcg agcatctgct
    11941 ccaccttctt gcgctcggtg atgtctcgcc cgacaccggt gacaccgatc acccgccctt
    12001 gcaaatcccg atagggagca ccggtccagt gatacagcat ggtccggcca tctttagcca
    12061 acagatggcc ctccaactcg gcgtagccct tctcgtaggc ttcgcggatc gccgcggctg
    12121 ttttttccgc ttcatcggaa ggcaccattt caagcgccga cttaccctgc aactcctccc
    12181 tcgtatagcc cgtcaccgtt tctaatttcc ggttccattt ggccactcgc ccctcgagat
    12241 ccaacgcaaa aatgatgtca gggacggttt ccatgatcgt ttcaagatga ctgagcgcct
    12301 catcgcgttc acgctcagac tgcttgcgct cgctgatatc ggtaatcgtc ccgaccatgc
    12361 gcaacgggcg gccatcctcc gctctggcaa cgactttgcc gcggttcaga taccatcgaa
    12421 actcaccgga tttctgtcgc agccggactt cgatctcctt gaggggaatg cggccgtcca
    12481 cgtgtgcctg caacacgtcc atcatgcggg cgacatcatc gggatgcaga agggtcataa
    12541 aaaatgcccg ggtgggcagg acttcttcgg actgatagcc aagcaatcga atccactgcg
    12601 ggctgtagta cacctcatcc gtccgcatgt cccagtccca catgccatcg ttggtggcct
    12661 caatggcaaa gtggaaccgc tcttcactgg cccgcagtgc gcgctcggct gatttgcgtt
    12721 cggtgatatc ccgcacaaac gcgaaatggc attcgacccc gttgtaatgg acaaagttca
    12781 cattcacatc gatgggaatc aggcggccat ctttggcacg atggcaactc tcaaacgaga
    12841 cacggccccg ttctttggtg tgtgcccaaa aggttggcca tgtttcagcc tggaagttcg
    12901 ggttgatgtc gtggactctc atccgccgaa attcgtcttt cgagtagccc agcatggaac
    12961 aggccgaatc gttcacatcc agtaattccg cgtcggcgcc gacccaataa atggcctcgg
    13021 tcgcgctatc aacggtgtat ttggccagct ggagcgcttc ctgggcgtgc ttccgttcgg
    13081 tgatgtcctg taccattgcg agggtataga gcggggtgcc gtcctcgtcg cgtatgacgg
    13141 atacacccac ctgggcccac acgacgcctc cgtccttcct gacgtatcgc ttttcaaaca
    13201 catcatagac ccgtctgccg gacaggagcg tctgaatacg atcaagattc tgccccagat
    13261 catcaggatg ggtgattgac tgaaacgtat tggccagcag ttcctgctct gaatagccga
    13321 gaattttaca gagagcctgg ttgacctcct ggatgcgacc tcccacgtct gcgatggaca
    13381 tccccactgt cgcattgcgg taggccgcac gaaatcgttc ttcactcgcg cgtctcgcct
    13441 gttccgcctg cttcgcttcc gtgatgtcct tcacgtacgc gacgcgtgac accggcactc
    13501 catcggcatc cctgatgagc atgacctcga ccatcaccgg gaaaagactt ccatccttgc
    13561 gccggtgaat ggattcgaat gtcaggccgc ccgtgtcatg aatgcgctgg attcgctctt
    13621 ccagggcggc atgttcctct gggggataga tgaaaaaaat tgagcgaccg atcaattcct
    13681 ccaccgtgta cccgcattgc cgggcgaacg ccgggttcac ggccagaaag gtattggttg
    13741 ccaaatccgc gtgggcaaga ccgagctgtg cctgatcaaa cacctgttgc cagcgtcgcg
    13801 cgacgtcttc agcctgcttg cgctccgtaa tatcggttga cgccccgacc atacgatagg
    13861 cgcggccctg gtcatcccat aaggcctggg cccgtgtctg aacccatcgg tacgtgccgt
    13921 ccttgtgtaa cagccggcat tgcacgtcat acggggtccg gcgggcgaaa tgtctctcaa
    13981 tctccgcttt cacccgcggc acgtcttcgg gatgtacgcg ctgaaaaaac gaggattcgt
    14041 gatccggcaa ctcgtcttcg gcaaacccca gcagtgcctt ccagcgcgga gacagaacgg
    14101 cctgaccggc aagaacattc caatcccaga tgccatcaga agtccctcga agcgccaggg
    14161 catagcgctc ctcgctctcc ttgagctgtt cctctgcaag atggcgaccc agttcactcc
    14221 ccgcacgacc ggcggccaca cgcagcaagg cttccgcccg ttcagcatcc ttcagggggg
    14281 atcgaccgag cagcgcgatc cagccaactg gccctccagt cggcgagggc agtgtcagag
    14341 ccatgtacga ttcgatggac aggtggacca acacctcgtc ctgtgggaac aaggcctgga
    14401 cgccggaaga gtagtggcag aatccttcat ggaggacatg ctcgcagggg gtcccggcga
    14461 gggcatagtc cacaggatcc agccggccgt tcggccccca cccggccacg gaatgcgcgc
    14521 atcgcccttc gggcagtgcg cgaagacaga aaccgtagtc cgcgccaatc gtttttacca
    14581 gggtctccac caggtcctga aggaacgccg tgcccgtgag cctggtggtg ggggccatca
    14641 ggcgcagcac ctcctcattc cgtttctgaa tagtgagatc tcgcaccgcg acgatgccgc
    14701 cgctccagtc tccaccagac cagcacggca catggttcca gcgcacctgc agcagagagc
    14761 catcaccgtt cacgaacaca tcgtcctgtt cgagcacggc ggtcccgcta aggcaggcat
    14821 gcatcaacgt gcacgatccg tcctgcccct cttcatcccc atcgccagga cggtgcacac
    14881 actcatgcaa caaccgcccc ctgaggtcag aggctaaaac gcctagaaga cgctctgccc
    14941 cccgattagc ccgaagaatc cggccgctcc gatcgatcac gaaggccgcc tctgtcaggg
    15001 tctccacctg atcatccgtg gacagcgagt cgacggatga tcgttcggac ctcagcatgc
    15061 gcagttccag gagtgccgca gtctgccgcg ccaatcgtcg aatggtcagg aggtcgtcct
    15121 ccttcaccgt ccgcggaatc gaatccatta cggcaatcgt gccgaccgca tacccgtctc
    15181 gagtgacgag aggcgcgccg caataaaatc tgacatgcgg atgtccgcgg accagcgcat
    15241 gggatgaaaa gcgatcatcc gccgaggcat cctcgatgac catcggatct ttcccttgaa
    15301 tggtaaaaga gcacagccca gacggacggt cggtttggct ggtgtccagg cctacagcgg
    15361 ccttcaccca ctggcggtcg tgatcgatga acgtgatggc ggcaatcgga gccttataga
    15421 tcgaggcggc cagttgcacc atctcgtcga agtcggcttc tggaggagaa tcgaggacgc
    15481 ggtagcggca cagggcccct acacgcgcgg tttcatcggg aggcagcgta tgcgtcccca
    15541 tgcgtgagct cccacgcgcg agagaatgaa atggctgtat tcatacatcc ggccgagtca
    15601 tatcacaaat acccaagatg gactagggcc atctggaacg acactcttcc tgtagaggcg
    15661 accgcgcgag ccacctctgt ggactggcta caaccaagcc ggggcagagg tgccgtcggc
    15721 gccgcgccag gcgggcaccc acgccagtcc caccagtttg acgatgatat ttgacttggt
    15781 gggctttagt gaaatctggt cgagcttttc agtcaaggga tccgtcgccg ccatgagcgc
    15841 atcgctttcc gatttgaact gggcctccag gtccgtcagc tgttgctgaa gcgtggccac
    15901 gttttcttcc gcatggccga cctcttgcga ttccttcatc acacgccccg cgctgcggat
    15961 cgccgttgtc gcacggccga tgttcgtcgc gctgagtgtc ttgcggccca gaaatgcacc
    16021 cagtatcgat gcgccgacag aaatgaccgc ctgcacctga ctggatcgag attcgttctg
    16081 ttgtttctcc cgggcctgct cggcgcgccg gacccggtct cgaagcgtcg taattttggg
    16141 cgcatatttt ttccgaagct cgtcgctcag tctgtcacgc tcttcccggc caacctgctg
    16201 taaccgtacg cgaaaatcgc gttccgactc gccgggcttc gatagcgtat ttgaggtcgg
    16261 acttcgccag agttcgagct tttgcgtacg aaacaaccag ccggaaaggt cgctcttcca
    16321 gctgtcgtat tgcttccctt tgctcgctgc accgggcaga gccgcgaact ccgcagcggc
    16381 ggccggttcg cgttcaagat ccgccacctg cacatccgcg gccgtcgact gttcccactc
    16441 gaccggaacg acgccactgc tgatagcagc cagtcgcgtg acatcgttcg tggcctcgat
    16501 ccctgctttg ttgtcggtaa atcgcacctg cgcagcaccc aagaccattg gctgatagat
    16561 gagcgttgcg ccacttggtt gcgttccccg gagcggaaga aaatattgcg gcacatcggg
    16621 aggcaacata ggccgggagg ccgtcgtgcg cgacgccgca ctcgtcgagc cggaagcccc
    16681 tgcggctccc agttcgtcct gccgtgaggt ccgcgctggc agctgacgct tcaccggatc
    16741 catcaactgt ttgatctgtg tgcgtgtcag tggcccccgg agatatgaca gcgtccagcg
    16801 tgtttgaaag acttccggcg catcttcatg cacgttattc aacagaaaga cccgattgcc
    16861 cagaccggca aggatctgtt ccatctgctg gcggtcgaac ttcttaccgc tactggccgc
    16921 cgctccttcc aagccttcga tcacccgcgc cttatcgcgt tccgtctgca aacggccgat
    16981 gaaccaggtg ccggtattgg ccagcccctt ataatcgaga tccaccggat tttgcgtggc
    17041 caacacgaca cctagcccga acgcgcgcgc ctgcttcaac agcgtcaaca gaggcgcttt
    17101 ggagggaggg ttcgcgaccg gcggaaagta gccaaaaatt tcgtccatgt agaggatcgc
    17161 gcgcaggctg gtggtgccgg actgcgaacg gacccacccc aatgtctggt tgagcagcag
    17221 tgagacaaaa aacatccgct ccgcgtcgtt caaatgggca attgaaaaaa tcgcgatgcg
    17281 cggcttcccg gacggcccgt acaaaatctg tccgacatcc agcgcctcac cttccatcca
    17341 ggcggcaaac cccggcgcgg ccagcagatt gttcaattgc atggccaggg cgaatcgttc
    17401 tttcgaaggg aagaatgatt ccacatccag taccccgact ttggtcatgg gaggcgtttg
    17461 aatctgatgg attagcgccg ccaggtccag gtcacgccca gcccgccagg cactgtcaag
    17521 aatcgacgac aacaatatat gttcacgact tttgatggga tcagcctgga gaccgatgag
    17581 tcccaacaca ctggtaaccg tcgtggaaat cctctcgcgc aaaagttccg catcatcgag
    17641 gaactcgggt gaaggcgccg caaaggattt gaggatcgac accggcagac ccgcattgct
    17701 gccgggcgta tagatgacga cgtccgccgc atcgcgcaac ttttgaatcc gctcgccgct
    17761 ctgaccccat tcgcctaagc ctttctgcca caacgcagct tgttgggtcg cgtagtcggc
    17821 aggcgaaagt cctttcttgc gggcatcgtc ctcgttgatc cagggggcaa agtcttcgcc
    17881 gcgcagttgc gggaacgtca ggagaagatt ggagagatct cccttgggat cgatgacgat
    17941 ggccggaatg ccatcaatgg cggcttcttc caagagtccg acgcacaggc cggtcttccc
    18001 gctccccgtc atgcccacac acacggcatg cgtcaccaga tccttcgaat catacagcag
    18061 ccatcccggc ttggattgct tcgtcgcgag cgcatagggc cggccgagat aaaagacccc
    18121 gagcttctca aactcctccg tcggttgccc ttggttcgct gcggcagacg ggcgagccgt
    18181 tgatcccttc ttggctggca taattcctcc tcgcatggca atgcacgtgt gaccggattg
    18241 gtcgaggcca ttgtattgat tgacagaaat gtccgcaagc aacggcaccg gcattcgcct
    18301 cagcaaactc gggggctcct agggccaaac tagggaggcc cccagtgtgg gtcttcgtct
    18361 cagtttcata cagtcaaacc ctgacaggcc ggactttttt accgtcaacg aggggagatt
    18421 ggacatgagc agagggtggg cacttggaat cgcagtcttg ctgaccggat gcgggcctca
    18481 ctacaccatt ccaaattacg acacgaatga aatcgaaaag gtgatttcca gcggccgctc
    18541 tcctcaggga tgtatcgaaa atctcaagga ggatgccaag aatttgaacg tggacgttcg
    18601 cctgaccgac gtccagcagc agccgtcctc gggccccatt tcctggatct acacgaacac
    18661 gtacatgtgc acggggaaag tggtggccaa acgataattc acgaaaggct gacacgtgaa
    18721 caccgtatcg atgcggcact cagcgacaaa cgaggggttg ggcgggcgac ggcctgcaga
    18781 tgcgcctctg accctgcgct tcctggtgac ctttgatgac gggccccatc ccaacaccgg
    18841 gaaagtcctg cggcacctcg cccgcaatcc ggtgcaaccg cacatcaaag gaatattttt
    18901 cgtgcagacg cgccacccga agcgaggggg ctcatccgat gggcgagcca tgctggcgat
    18961 tgaacaagcc gaggggcacc ttttggggtt gcatacgggg accacgaaag ggcacctcag
    19021 tcacactcgc atgtcgccgg ctgaactcga gcggtcactg ctcaacggca aagaggacct
    19081 cgaccagatc accggcaagc cgacgatgct ggtccgccct ccatactggc tgttcaacgc
    19141 gtcaaccttc gactgctatg gacggcatcg gcttcacatg ctgttgtccg atgtcaaagc
    19201 cttcgacggg gtgaactggg gcatccacct gctgcgaagg ctgaatttcc gctcgcagct
    19261 ggctgggtta catgcccgct ttcttcgggg aacgttgccg gtggtgaatc acaccattcc
    19321 catcgtggtg acctttcacg atacgaactc cttcacggca gaacacctcg gcgactatct
    19381 cagccttctg gtcgaggagg ccgcgcggtt gggcctcccc ttacattcca agcccttcat
    19441 cgatgatccc gacgaattat tagccgcggc gcttcggtgc gccaaaacca gcaggggtac
    19501 ggagcgcaat ccggccctta tccaggaggc gcagtgatat ggcgttttcc tcgcaatccc
    19561 aggtgggcgc cgacggcgcg ttggagcagg aactggccac cctcatcgtg gaggcactcc
    19621 gcttggaagt gttgcccgag accatcgatc cgtcctcggc actcttcggc gaggggttag
    19681 ggctggattc catcgacatg ctggaaatcg ccctgttggt gtccaaacgt tatggcgtgg
    19741 aattgcgctc ggaaaataag gcggccctgg cttcgctccg ggcattggcg aatcacattg
    19801 cccaacatcg gattcgatga tgatgcaatc gatcgacagc cagatggctc cggcctcccc
    19861 cgcccggcgc acgcaccagc cgagagcggc agccggatat cctctcgtag gacatcatgc
    19921 cgcaacgagc ccgctcgcct ggcgccgtga tcgtgcacat tccgttcacg agtttctgtg
    19981 tgctgcgata agtctcgcgg cgcagttgcc gcgcaaacac tttgccatca atctttgccg
    20041 ggatcgctac cacttcgccg tgggatttgc cgcggccttg atcgcacgtc agatcagtct
    20101 gttgcccacc tgccgcgcgg cagaacccct tcaacaactc gcgcgacagt atcccggtgc
    20161 atatgtgctg accgatcatg acgaggggga tatccccata cctcgcttct ccctccttcg
    20221 agaaatggag cagggggtcg gtccgaaaga tgttccggtg attcccgccg atcagatcgc
    20281 cgccatcgcg ttcacctccg gcagtagcgg gttccctcag gctcatgcga aaagctgggg
    20341 atccctggtc atgggcgcgc gcgcattggg gcaacagttc ggcctgacgc agtcttcgtc
    20401 gagggtggct gtgggaaccg tccctgccca gcacatgtat gggttggaaa ccacgatcat
    20461 gctgccgctt caatggggat ggagtgtgca cgccggcact tcaatcctcc ccgcggacct
    20521 tcggacggac ctggagcaac tggaatcgcc ggcctggttg atgacaacgc ccttccatct
    20581 caaggcgttt ctccgcgaac gcacgaacct gcgcggcctt gagggcatca tctcggccac
    20641 gatgcccctg gaccggtcga tcgcgctcag cgcagaacgg ctgtggcatg tgcccgtaca
    20701 tgaaatctat ggatgcacgg aaggagggat gctcgcgtct cgccgcacaa cggccggtga
    20761 agcctggacg ctgtgccacg atttacggat gcggcatgcc gaggacacga cctgggtcat
    20821 gggcggacat gtgggagcct ggctccaact atcggaccgc atcacgctcc gcagtcagcg
    20881 ggaattcctc ctcctggggc cacaccgtga cctcgtgaag gtagcgggaa aacgcgcctc
    20941 cttgacggcg ctcaatgcaa tcctgaaccg cattcccggt gtggtggacg gcacgtttta
    21001 ttggcccgac ccaggacaaa caggcaccgg cagattgaga gctttcgtgg tcgcgccggg
    21061 gatgactcgt gcagcgattc ttgccgagct ccggaaacgt atcgacccgg tctttcttcc
    21121 ccgcccgtta tatgtggtgg atcagctccc caggaacacc accggcaaat taccacacga
    21181 acatttgaaa acctttgccc gacacatcat gcatccccaa cagcacgttc acgagtaacg
    21241 catgcctacc gaaagaccgc gacagatccg ccgggaccac ccggctttgg ccgggcattt
    21301 tcctggcaac cctctcgtcc cgggagtggt gctcttaacc gaattgctga gcatccttga
    21361 acaagaagcc ggtatgaaac ctggccccct cgaactgagc tcggtgaaat tccttcgact
    21421 gcttcggcct gaagaaccct tcacgctgcg gtggcgattc ctgcctaacc aggacatcgc
    21481 ctttgccgtg acgcgtggtg aggacatgat cgccacaggc acggtccatc gcgctcgcgt
    21541 caccacgcag gtcgcatcat gagcaccggc tggcgccatc agccggaacg aggaagtcga
    21601 aggctgatgc ggctgctcgt cttgattacc ctctgggtgg gacggccggt agcgcgcggg
    21661 ctgctctacc cgatctgttc ctatttcctt ctcttctccg gcggtgccca acagacttcg
    21721 cgcgactacc tgcggcgcat tttgcaacgg cctccgtcgt ggaaagacgt ctttcggcac
    21781 tttcacgcct tcgcctcaac gattcatgac agggtgtatc tgctgtccgg acgccatcgc
    21841 tacttcgata ttcacaccga aggcgcttcg gcattacatg ccgttctcgc ccagaatcga
    21901 ggctgcattt tgctgggttc tcacctcggc agtttcgaag tcctgcgcgc gcaaggcatc
    21961 ttcgatcaca agctgccgat cacggtgatc atgcacgaag ccgcagcctc caacttaaac
    22021 gaagtgctgc acaacctgcg ccccgagatc cgggaccgga ttattccgcc tggaaccgcc
    22081 gacacgatgc tggcggtcaa agaacgattg gatcgagggg aaatcgtggg gattctcgga
    22141 gaccgcctgt tcaacgatga aaaaggcatg gcctgcgagt tcctcggcaa aacccgcatg
    22201 ttccccgaag ggccatttct gctcgccgcg ctgctccagg tgccgatcgt gctgtttttt
    22261 ggtctctacc aggggggccg ccgataccac ctgtattttg agcccttggc cgacactatt
    22321 cctgtgaatg gcgcccgccg atcggaaacc ctacgccctt ggatcgagcg atacgtcagc
    22381 cggctggaac attactgccg tgtatctccc tataactggt tcaactttta tgaaggtccc
    22441 gatcacgctc agcgctctca tcctgctgtg tagcttgtgg gcctggctgc cggcgtccgc
    22501 tgaacctgcc atgggagcgg agccattcga cgtgcccgga ctgatggaac tgctggccaa
    22561 ggtctcgtcc cgccaggacc gtttcacgga aacgaaaacc atcgccatgt tgactcagcc
    22621 ggtggtgctc aaaggcacgc tggcttatgt tcgtccagac cgcgtcgaga aacatatcac
    22681 ctcgccctat acggaggatc tcacggtgca aggcgatcgg ctgacgctgt cctcaccgaa
    22741 gggcacaaag cagattgccg ttgatagcca cccgttgatt cgctccttca tcgaagcgat
    22801 ccgggccagt ctggcgggag aacttgccgt attgcggcgg ctctaccaca tcaagtttca
    22861 gggcacccgc cagcagtggg aactcacgct gcggccgtta gatgcgcaag cggcggaata
    22921 cgtggcctcg atcatccttc gaggagaggg cgatcgttta acaacggtcg acatacggga
    22981 aaccggaggg gaccggtcct tgatggagat cgatgggccg gtctcgtgac cctgcatgcc
    23041 cgaccggccc tgttcctctg gatgctgctg gtgtttatca gcggaggaat cgttgccaac
    23101 atgacgatga ccaccgactt gaccgccttg ctaccccgtt ccgctgacca tcggcaggaa
    23161 ctgctggtgt cgcaactccg ggacggcgtc gcggcccgac tgatcctgat cggcctctcg
    23221 ggcgccgagg gcgaggcact ggcccaagcc agccgaacca tagcccgcaa attacgagac
    23281 acgggcctct tcgcctcggt aaacaacgga gaccccgccg acttcaccat cgtccgtgag
    23341 gtgctgatgc gccatcggta ccttctgagt gcggccgtca tcccggagca tttcacggca
    23401 acggcattgc gggaggcatt ggaacgacag ctgcaatttc tcgcctctcc ggccggtgcg
    23461 ctgatgaaga ccgccttgcc ctccgatccc accggtgaag tgcctcacat actggcggcc
    23521 tttaccccgg gggagggacc ggcaatgcac cagggcgtct ggttttcgcg cgacggaacc
    23581 agagccctgc tcgtcgctga aaccagctcg ccagggtttg ccctcgatca gcaggagtcg
    23641 gcagtgcagg ccgtgcggac ggcattcaca tcagccggcc tgccggagca tgcccatctg
    23701 gacatgaccg gccccggcgt attcgcggtg gaatcccgcg ccacgatcca gcgcgactct
    23761 tggcgactgt ccgtcatcgc catcacagtg gtgacgattc tgctgtttct cgtctaccgg
    23821 tcggtgacgc ctctcctgct gagtctcctt ccggtgttga ccggcttgct cgtcggagtg
    23881 gcggcggtac aaacctggtt cgggttcgtc catggcatca cactgggttt cggcgccaca
    23941 ctgattggag aagcggtcga ctaccccgcc tacgtcttca cacagggcat cgcaggagaa
    24001 cgactgcagc agaccctgtc gaggatatgg ccgacgttga aactggccgt actgaccacg
    24061 gtcttcggcg cgctcagctt gctgttttcg agtatcaccg gcctggcgca gttgggcgta
    24121 ctggcattcg tgggggttct cgtagccgga ttcgtgacgc gctggatcct tcccgccctg
    24181 ccgcccggag tcatccggtc cgctccacac cagctgcttc cgatgtcatg gctgcctgct
    24241 ctggcatcgg tgaatcgcat tcgctggacc atatgggtgg cggccctgct ggccctggcc
    24301 accctggcaa tgcgacatga aaacatctgg gataacgacc tcgctaattt gagtccgatt
    24361 gcatcgccgt caaaaaccct cgatgagcaa ctgcgaaaag acctcggcgc gccggatgtt
    24421 cggtatgtgc tgatcgtcga gggctcgagc aaggaagagg tcctgcaacg cagtgaatcc
    24481 gctgaactgc ctctgcgccg cttggtggac gacggactgt tggccgggtt cgacctgccg
    24541 agtctgtatc tcccgtcgga tgcctctcaa cgaaaacggc aggccgcact gccttcttcc
    24601 gatgtgctgg ctgcatcatt ggctgaggca gtgaaaggac ttcccttcag ggccggactg
    24661 ttcgagccgt ttttgcacga tgtcgagatg gcccgcacga gtccattgat cgatatcgtc
    24721 aatcttcaac agacgccctt ctttctcaaa atccggtcgc ttctggttca cactgatgat
    24781 gcgtgggcgg gtctcgtgcc cctgcgccac gtgacctctc cctccggtct ggccgaccgc
    24841 cttgctcggg aggcggggcc cggactcact ttcctcgatc tgaaatccga agcggggcgg
    24901 ctggtcaacg ggtaccggca tgaatcctta cggctgaccg cccttggagt ggtggccatg
    24961 acgatactcc tgtggtgggg gctgcgggac atggcgctgg tgagccgcgt cctgttacca
    25021 ccgctactgg ccatcctgtt cgtcgtcacc ctgctcaccg tgatcggcga acgcctctcc
    25081 ctctttcatc tggtgtcact cttgctcgtg ttgggcatcg ggctcaatta cgcgctgttc
    25141 ttcaaccggc ccttctcaga tgaggaagag aaacggcgga cctggctttc gctcacggtc
    25201 tgcatgctgg ccaccctgtc ggcgttcggg gccctggcct tttctcgtac acccgtgctg
    25261 catgccatcg ggctcaccgt tggtctcggc gcggcagggt ccttactcat tgccgcgatc
    25321 ctcggccaac agcgggtgtc gcaatgatgg ctccattgac cgtcaccgcc atggccaccg
    25381 ccaacccgct cggactgggg ctggcggaga cgtggcaggc gctgcgcacg gagcgacagg
    25441 gtttgcggcc gaatgacttc gaagatgcgg acatcaagac ctggatcggc cggatcgaag
    25501 gactcgaaac ggaaccgatt accggcgaac tcgccgattt cgattgccgc aacaatcgat
    25561 tggcgctggt cgggttacga caggatgggt ttgaacaggc ggtcgcctcg gcgcggcagc
    25621 ggtacgggcc ggatcgcatc ggcgtactca tcggcacgac gacctccggc attctcgaaa
    25681 ccgaatgggc ctatcgacgc cgcgcggccg ggggtgaatt gctcccgctg actgtgcgct
    25741 atcgccacac acagaatttg ttctccgtga gcgatgtcgt gcggcgtcac ttgaaactca
    25801 ccggcccggt cgcatcgatt tccacggcct gttcctcaag cgccaaagtc ttcgcgagcg
    25861 cctcgcgctg gatggccgcg gggctctgtg atgccgtcgt ggtgggcggc gtggacagtt
    25921 tgtgcttcat gacgctctat gggtttgcct ccctgggcct gctgtcgcct cgcccctgtc
    25981 gtccctgcga cgaacatcga gacgggcttt ccatcgggga agccgcggga ttccttttgc
    26041 tggaacgcga gaccacccgc gacagcggtt tgcggtttct gggttacggc gaaagcagcg
    26101 acgcccacca tatgtccacg ccgcacccgg aaggcctggg tgctgtcttg gctatggaac
    26161 gcgcgctcga ccgggcggag ttgaaaccca tcgatatcga ttacatcaat ctgcatggca
    26221 ccggaacgcc agccaatgat ctcgcggaag accgggccat ctggaaaacg ttcggagaaa
    26281 gaacgccgtg tagttcgacg aaggagagga ccgggcatac cctcggcgct gccggaatca
    26341 ccgaagcaat tctcagcctc ctctgcctgc ggcatgaatt tattccaggc atggtgcgga
    26401 tcgagcaacc ggatccgcaa ctccgcagcg gcatcatgct tcgaagcgaa tcccgtccct
    26461 tgcgcaccgt gatcagtaac tcattcggtt tcggtgggag caattgcagt ctcattttcg
    26521 gaaagaggcc atgatggagt tctgggtgat cagcatcggg atcgccggcc ccgggctgga
    26581 cggatgggct gctgctcgac ccatcctggc cggtgaaacc gaataccggc ctgcgcctct
    26641 cacacctcct aatctcaagc tgttgtccgc cgccgaacgc cgccgctgta gccaatccgc
    26701 acgtcttgcg atcgcggcgg cgcaggacgc cttgctggca ggacctctcc agggagaggc
    26761 gatcgccacc gtctttacgt cttcggatgg tgacggggcc atcacacaag agatatgcga
    26821 atcactggcg ggtgccgcac gtgaggtatc ccccacgtcg tttcataact ccgtctacaa
    26881 cgccccggcc ggctattgga gcattgcaac ccgctcgcaa ctctcatcga cgagtgtctg
    26941 cgcctacgat gtgtcgttcg ccgccggact cctcgaagcg gccacctata ccaccgtcga
    27001 gcgctgcccg gtgatgttgg tcgcgtccga tctgcccttc cccaggcccc tgtatggaat
    27061 tcgccccgtg gaagaaagtt tcgccgcggc gcttttaatg actccgacaa gccatcccga
    27121 tgcgtgcatg cggtgcgaga tcgcgctggc cccgcatcag gccatcacgg cctttcccgc
    27181 taccataccc gagtcgctgc gcagcaaccc ggcggcgcgc agcttacccc tgttggccct
    27241 gctggcccga caggccgcag acaccgtgcg gctcgagtac acggacgaga gcgacctcgt
    27301 gatcgactgc aaaccggagg gtcattgatg gaacgccgct gtgacgacat cgccgagctg
    27361 gttcctcatc gcggaaccat gtgcctgctc gactcagtcg aaacatggga cgaacaccat
    27421 atcgtctgcc gtgccacgag ccaccttcgt ccggacaatc ccttacgaga cggcagcggc
    27481 cttcgtaccc tctgcgcgat cgaatacggc gcgcaggcca ttgccgcgca tgcgacgttg
    27541 ctcgggagtg ggcatagaca acgcgcggcg gaaggtgtgc tggcttcgct ccgtcatgtc
    27601 actacgacgc agtctcactt ggatgggctg aagggtgcgc tcacgatcct cgcgatgctg
    27661 cagttgagcc accagcaagg ccgtatctat gacatcacgg tgaccgcaga tggacaaacg
    27721 ctgttgactg gtcgcctatc cgtgatgctg gaggcggatc attctggcga ggctcgatcc
    27781 gtctcacagg aaggagccgc atgagtaagc gtgcgctggt gacgggaggc agcggcggca
    27841 tcgggcaagc cgtctgtcag gtgctgggcg aggccggatt cgaggtcatt gtccacggat
    27901 gcacccgcat ggatcgggcg gaggccgtgg cgcaggccat ccgtgacaag ggcgggaagg
    27961 ccgaggccct gtgcttcgat gtcacggatg cagcgcaaac ccgccaggcc ttagatcgcc
    28021 tgcaaccagc cggccccatt caagtcctcg tgaacaacgc cggtatctat gacgacgcgc
    28081 cgatggcggg gatgacgaac gaccagtggc acagggtgct cgatgtgtca ctgaacggat
    28141 tttttcatgt gacgcagcca ctcctgctgc ccatgatccg gacccggtgg gggcgtatcg
    28201 tcaccatcac gtctgtcagc gggctcatcg gcaatcgcgg ccaggtgaac tacgccgcgg
    28261 cgaaagccgg tctccatggc gccacgaaag ccctggcgct ggaactcgcc tcacgtcaca
    28321 tcaccgtgaa tgccgtcgcg cccggcatta tctccacgcc ggcgtcgctc gacacattcg
    28381 gcgcagaagc catcgagcga ctggtcccga tgaaacgagc cggcactgtt gaggaagtgg
    28441 ccggcctcgt ctcgttcctc gtctccgata cggcggccta tgtatccggc cagatcatct
    28501 cgatcaatgg agcccaggca tgagggaacg ggacaaggac gctattgcca tcatccccgc
    28561 ctacaatgaa gccggaacga ttcaccatgt cgcctcccgg actttgcatt acgtcgagcg
    28621 ggtcgtcgtg gtggatgatg gctcaaccga tcagacggtg tcgtcgctgc gggaattgcc
    28681 cgtgacggtc ctgcgacatg cgaacaattt gggcaaggcc gccgcgctcc gacgagggat
    28741 gcagcatgcc gtcgcgcaag gggctacggc gattatcaca ctcgacgggg acggccagca
    28801 tgatccagcc gccatccccg ccttactcgc catgcaccgc cgggacccgc gcgcaatcgt
    28861 cgtcgcagcg aggcgccggc atcgccacac gattccgcga ctccgcgtac ttgcacagcg
    28921 gattgcggac ttctggatct ccctggcagc aggctgtacg atcgaagaca gtcagtctgg
    28981 atttcggctc tatccgattt ccgcctggac ggcggctggg tggccttgcg atgcggcttc
    29041 cggattcgtg tttgaaagcg aaatcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnngtg gtcggagact ttcgtctgtc gctgtccgtg agtgccagag tcgctgccgt
    29221 tccctacctc acggtgacca atgcccattg gagcccgttc gcacaccagc atttcgtcgt
    29281 tcccgaattg gccatcacag agcgcttcgg cccgcgcctg ggacaagcac tcttcaccat
    29341 gatgcgtcca tttgttttcg cccagcaggg acatgccttg aaccaggttc gccgcgacta
    29401 tggattgccg gcactcccgt acagcctgcc gcaccagttc acagacgccg acgtgacgct
    29461 ctacgccgat gtgccggagc ttgttccgac ctatcatcga ccgccgcacc atcactatct
    29521 cggtccggtt ctctggtcgc cggactcggt tcctcgatgg tgggactcat tgcctgatac
    29581 gggacggatg gcctacgtca ccctcggcac gtccggacgg gccgacctct tgcccctcat
    29641 tctggacgca ctggagcaac ttggtatcga agccttggtg tccaccgccg gtcgctccat
    29701 tcccggacgg cttccacccg gtacgcgggt cgcacagtat ctgcctggcc aagcagccgc
    29761 cgcgcgagcg gatctggtca tttgtaatgg agggagcgcg accgtctatc aagccctggc
    29821 ggccggcaag ccggtgttgg gtattcccaa caacctcgat cagtacttaa tgatggacta
    29881 cgtccgccgg agcggcgcgg gcgactacct cagagcaggg accatctccc gtgccgccgt
    29941 gacaatgctg tgtgacggct tgttaggcaa ccccgccttc cgcgatcgag ccggccgggt
    30001 acaaaccgcg atcgagcaga accgcacagc gagagcattc gacgatcatt tgaggagcgt
    30061 attgggaacg ggcaggtctc atcaggctcc gtccaagatc cggacgccga aggtgtcggc
    30121 cttcgattct gttcccgaag aaagctctcc atccaatcgg gcgagtcggc gatgaatcag
    30181 acctgtaccg tccatgaccc aatcccgccc caagatgccg ctcaaatgga gcagcgggtg
    30241 gaagaacatc tcacggtgca ctccagggcg ctcaccggcc tcttcctgct ggccgtcctc
    30301 tatacgttgc atttcggaca ggtgatcttc ctgcccatga cgattgcgtt cttgttggcg
    30361 gtgctgtttg cccccctcct gcgtcgcctg cggcagtggc atctgccgga gcctctcggg
    30421 gcagcgatac tcctcgcact gttagtcagc ggcctcgtgt atggaggctc gcgtctggcg
    30481 gaaccggcag cggagtggag caccaaagtg ccggtggcat tgcggtcggt tgaatacaaa
    30541 attcagccgg tcaaaaaatc gatgcaggat gtgacgaaag ccaccgaact gnnnnnnnnn
    30601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctgctcgga atgccaaacc cgatcctgtg
    30721 gggggtcatg gccggcctct taactttcat tccgtacatc ggccacatct tcggcatcgt
    30781 ggtggtgatg ttggtggcgg cgttgacgtt cgacgatctc ggacaaatgt ttctcatcgg
    30841 cggtgtctat tggggcctgg ccattctgga aggatccttc gcgtcgccga tgattttggg
    30901 gcggcgcttg gaaagcaacc cggtcgtcct ggtcctggct attatgatct ggggctggat
    30961 ctggggcgtc ggcggagcgc tcctggccgt ccccctgctc gctgcattca aaatcatgtg
    31021 cgatcacctc gaacccctgc aacccatcgg cgaatttctg ggcaagtagc cggcgcactc
    31081 gacagctacc aatcgtgcct tccttgatcg ttcctccgca aagaacagtt gccaacgtga
    31141 tggcgatact ggcaggatgc gcgcgcctat gaatcgtcga tggatcgttg cgctcgcctt
    31201 cgcgctctgc acaattccca gtctggtctt cgctgaatcc ccgccccaaa gcgtacattg
    31261 gggcgcgatc gcgtttcccg accatgaccc caccctgacc ctgagtaccg ccgtcctgga
    31321 tcggtttacg caattcgacg gagcagggag acgctataac gacattaacc agactatggg
    31381 gttcaacttt ttcaccatca gctggacgaa accgcttacc caattgcccg gctggaatct
    31441 caacctcacc gcaggcggcg gccccacccg tgacgggccg agccggttct tgcaaaacga
    31501 tgtcgtccat aagttgcgcg gcttcgaccc ggtgccggtc ggcgaaaaac gtgacgccaa
    31561 tgattttatg ctgagcggga ccatgacgcg ctggtttggg ctcttgggga ccagcgaggt
    31621 gtttttcgcc ggcgtaggcg gggcgggcgg atccttatat tatgagccgt acgttcaggc
    31681 aggatttcgc cgactggcga ttctcaatct gattcccgtg ctcggcgact atctccgggt
    31741 ctccggattg gcacgatatg gccggccatt cagcggcgcg gcgtttcacc aggtcgcccc
    31801 gcaatcgtac ctcgcccagg cgtcggtggg cttaggcaac tatcaccact ggaaagattg
    31861 taccccgtgg gaaattgaac tggctgtgac cgtcgattcc ggtctgttcg tggaccaccg
    31921 gggggacgcg ctggaagaac ggtttgtgtc actggcgttg cgctactccg cgttcacctt
    31981 cgaaacgtgg aacgacctca tcaaccagaa agattacggc ccgaccttcg gcgcccgcct
    32041 gacgctcgat ttgttctata tgtacgaccg ctggttccac tgaaggccgg accggttctc
    32101 ttcccctgtc tcactttccg caggtgatct tcagcgactc gatgtcgttc gccatatcca
    32161 gcttgcccag attcggcaca cgctgtcccg gcgcaaaggc gatttccgtc cccttgaagt
    32221 ctttgtcttc ataggcacgc accgtggcat tggaaccgac aatcacactt tcaatatcat
    32281 tactccaatc cttcccggcc agattcttca acgtggcata ctctttggga ccttggagca
    32341 cgacgtgcgg atcgtccaca tcgaagttat cgtcttcgaa aatttcgatg aagcaattct
    32401 tgtccacgac ctggacttcg agatcagcgg caaaagctgg cagacccgtt aaggccagca
    32461 ccgccgctac ggcaaatata tgttgtcgtc gcattatcac ccctccttaa tgtgagtctt
    32521 gtactggctc aacgcttggc acatcacttc aaaattccga gaaaccacag cagcacgaga
    32581 atgctggccg gcacgcctaa taaccagagg atgaaccctt tgcccatcgg tcccatggat
    32641 gcctccttgc gaatgctgct tcacggcaat aatgccgccg ccgtgagttc gcgattcaat
    32701 gccgacacca tcttgaggtg cgcactgtga tcgtcctgaa gatccgccgc aaataaatcg
    32761 gcatggttgc gctgcaggac ctgcccgaac tcctgctgct tgctgacggg aatttcgtag
    32821 agcgcggcca gtgcggtcac atactgccca ctgccggtcg cagcttcctg ccgcaggttt
    32881 tcataggcga cgcccgcgaa caaattgatc ttctgttcct tcacgaccat gccatcggag
    32941 gtgaacagat cattcgggct cgtgctggag aaaaagttga cggtggtatc gaccgtggct
    33001 ttggtggtgt tacaggcccc gaccatcaag ccggcggccg ccacacagag ggcacaggcc
    33061 aggttgtgaa ttgaggatga tgacttcata cagacctcct tggcttgtct cgcccacaaa
    33121 ggcgaaggat tcaacacagt gcacactttg ttgtacctgg ccaagggaga aggagagcct
    33181 agttattacc ctaggagaaa gaacagaaag gccctagcgg cttcaccgag ccggtgaatg
    33241 aacggttcga cagcgcgaaa cgaggaatga ctgggaaaaa gaaggaagga gcagacaggg
    33301 gaaatgccta acgaagcact ctcaccattc tttgaagacg acaaataccg caccgatcat
    33361 catgccgaat cccaccagat agttccattt gagttcctct ttgaggtaga cggctgagaa
    33421 gacgcaaaac accatgagcg ttatgacctc ctgtatcgtc ttgagctgcg ccgcagtgaa
    33481 ctcataatga ccgatccgat tagccggcac ctggaaacag tactccacga acgcgatgcc
    33541 ccaactcgca agaatcgcga tccacagcgg cgactccttg tacttcaaat gcccatacca
    33601 ggcaaaggtc atgaagatgt tggaaatggt cagtaagaga atcgtgtgca tgggcctcct
    33661 tgttcatcta tgattcaggg agatcctaca gcagaagctc gctctgcatc gcttagcgtg
    33721 acagcgagcc catcgaaatt tctagttcgc cgaaatcata aaccctcgcg caggattctc
    33781 cgttctttcg ttccactcca tgatatagct ttcgaacaat atctttctcg ataaacgtca
    33841 ttccatagtc ggcagaatcg atcttcagtg caattatctt tggatggtcg agacagtcag
    33901 gtccaaaggt ataggacggg cgcaaaaaat cctgcacctc gctattcgtc caaggggtca
    33961 gagccgcaat aactacccca aggggaacca caatccaccc tatgggagcg acataaacga
    34021 caccgacaat cccatgggtt tctattacaa atttcccatc gttttgtgtg tgtgctacct
    34081 tgcccacatt tctgacatga atggattcta cgggagacaa ctgtatccga acttcccgcc
    34141 catctaaagg ttggccctct tcgttgctca cttttccttc gaaccgaaga taccccgttc
    34201 cctcaccaac ataacagccg ttgagaagac cgagaaatcc cacaatcagg attcggatga
    34261 gtaagggcat tttcactcgc ctcaggggaa attgatccga tggtgccctt ttgctcgatc
    34321 acctgcgcgg gcggactgcg ccgaccctcc actgcgggcg gccaacctct tcctttcttg
    34381 cgggggtgga agggtggagc cttcagagtg cggtatggcg ggcgtggaga gatgtaccgg
    34441 ataacctgtc acagtgcggc gtcttgcaac ccttttgtcg cgcattagag tggcacgcgg
    34501 ctggaaccaa cgcatgactg tggactctca tggactgtcc gtctcttctc ctacctcttt
    34561 tttcctaatc acccgctccc cttctctctt agcggggtgt aagggatgga gcctttggaa
    34621 gtcggcatgg cgggtgtgga aagatgtagc ggataaacct gccgttgtgc gacggcatgc
    34681 gactttcggt cgcgcctcac agtcgcacac ggcaccggag cggatcggaa gcaacttcga
    34741 tccttcgcat catgccgact ccgaagactc catcccttac acccccgcct ctcttaacac
    34801 ctgtcccgta tacgaccgct tcaccttagc gatttctttc ggcgggcctt ccgcgacgat
    34861 ttcgccaccg cggtcgccgc cttctggccc gagatcgatg atccaatcgg cattgcggat
    34921 catatcgaga ttgtgttcga tcaccagaac ggtattccca gtctcaacca gacgatcgag
    34981 cacgtccagc aatcgttgaa tgtcggcgaa gtgaaggccg gtggtgggtt catcgagaat
    35041 gtacatggtg cggccggtgg ggcgtttgga aagttcgcgc gagagcttca cccgttgagc
    35101 ttcgccgccc gataaggtgg tggccgactg ccccagcttg acgtagtgca agcccacgtc
    35161 gtgaagcgtc tgaagtttcg acttgataaa ggggatattt tcaaaaaact ccaaggcgtc
    35221 atccacggtc atgttgagga cgtcggcgat gctgcggcct ttgtattgaa tgtccaaggt
    35281 ctcgcggttg tagcgctgtc ccttgcagac ctcgcaggtg acgtagacgt caggcaggaa
    35341 atgcatttcg attttgatca gcccatcgcc ctggcaggct tcacatcgtc cgcctttgac
    35401 attgaaacta tagcggccgg gcttgtagcc acgcacgcga gactcaggca aattggaaaa
    35461 cagatcgcgg atgaacgtga agagtcctgt gtacgtggca gggttcgacc ggggcgtgcg
    35521 cccgatcggg gattgatcga tgtcgatgat tttatccagc gcgtccacgc ccttgatctc
    35581 tttgcagccg tcgattttgg gcttcttgtg ataaagcagc tgcgacagcg agtggaataa
    35641 cacttcgagc acgagggtgc ttttccccga ccccgacaca cccgtcacgc aggtcaggag
    35701 gccgagcggc acattgacgt tcacgccttt taaattatgt ttcttcgcgc cgacgacggt
    35761 gacgaaccct tttggggtgc ggcgacgctg gggaagcgac accatttgcg cgccgcgcag
    35821 gtagaggccg gttaacgaat cggggttcgc cttgacctgt ttcggagtcc cctgggcgat
    35881 gattcggccg ccgtgggtgc cggcgcctgg gcccaggtcg agaatatagt ccgcagcctc
    35941 catcgtctcc gcgtcgtgct cgacgacgat caccgtgtta ccaaggtcgc gcaggcggag
    36001 taaggtctga agcaatcgcc ggttgtcccg ttgatgcagc ccgatcgacg gttcgtccag
    36061 aatgtacaac acaccgacca gaccggaccc gatctgcgtg gctaatcgaa tacgctgccc
    36121 ctccccgccc gagagcgtcg ccgccggacg atccaacgtc aggtagtcca atccgacatt
    36181 gaccagaaac ccaagccgct cgcggatttc cttcaggatg cgatgggcga tgaccaattc
    36241 acgttcggtg aatttgagcg tggcgaaaaa atccgccgcg gcacgcacgg agagaccggt
    36301 gatctcggca atagacttct tggcgatttt caccgccaga ctttccggct tgagacgggc
    36361 gccctgacag gtctcgcacc gttcaagcag ggtgaagtcg tcttcttccg tggcaccggg
    36421 agtcatggca tacccgatcc cgtcgcaggc cgggcaggcg ccgtgggggc tgttgaacga
    36481 aaatacccgc ggcgtgatct caggataact gactccgcat ttgatacagg cgagcttgtc
    36541 gctatagagg tgcagcttgc cggtctccga cagaaccccg accaatcctc cggccagctt
    36601 caccgacgtt tcgacggaat ccgcgatccg ccgcatcaag gcatcgcccg gcttcatcac
    36661 caaccgatcg accacgattt cgatggtgtg cttcttctgc ttgtcgaggg tgatgtcctc
    36721 gcccagatcg accatctcgc cgttgatcct ggcgcgcaca tatccggctt tgcgcatctc
    36781 cagcagttct ttgcggtatt cccctttccg accccggact atgggggaga gaatctgaaa
    36841 ctttgacccc tccggcagtt cgcagatggc atccaccatc tgctgtaccg tctgcgcggt
    36901 aatttcctgt ccgcattgga aacagaacgg gtgaccgaca cgggcataca gcagacgcag
    36961 atagtcgtag atttcggtga cggttccgac ggtggaccgg ggattgtggc tggtgctctt
    37021 ctgttcgatc gagattgccg gcgacaaccc ttcgatggag tccacatcgg gcttccccat
    37081 ctgttcgaga aactgccggg cataggcgga caacgactcg acataccgcc gctgcccttc
    37141 cgcatagatt gtgtcgaagg ccagggaaga tttccccgac ccgctcaggc cggtcatcac
    37201 gaccagcttg tcccgtggaa tctcgacatc gagattcttc aggttatgtt cacgcgcccc
    37261 tttgatgatg atggaattgc tcataagcgc gggattatat cacgccgggc aacgaggctt
    37321 ggcatccgac accttaaact tgacgctcat gctcagacga ccgtcaaaat gtcgacgcgg
    37381 tgttccagca tcgaacgatg gcattttagt tttccacgaa tgtgccgata cggaactgcg
    37441 ggaaccttcg ccccgatcca caaccacccc gatttcgatg gccgccatgc atgacgtgct
    37501 gagcctcttc aaacgcaaca tcaatcaact gttcgggatc accgtcgata tcctcaacgt
    37561 gggccgctct ctccaacaac tgtcgctctc catgcaaatt ctcgcgaata atggcgtggt
    37621 gcaagcggcc aaaatccctg ggggcaaagg ccggcccatg ctggcgctgg ttgagatcnn
    37681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag cggccaaaat ccctgggggc
    37801 aaaggccggc ccatgctggc gctggttgag atcttgaata acacccctaa ggaaatccgg
    37861 ccggaagtgg aagccctgga gcatctctgt gccgggctgg ccaaggtgac cgccagcagt
    37921 tcaaacattg tctggcgata ccatcaattg attgccagtc tgctttcggc gatgacacag
    37981 ggggagctct cttccaagtc gaagagcttg gagaccctct cgcaccttcg ctttaccact
    38041 gccgccgacg taacccaatt gacgcaacat cccgccttca cagcggtggg gggcctggag
    38101 cgggagaacc gcttgtatct ggtgcatcat tgccaggcga acctcactga gttacaggaa
    38161 cggctgaagg aggcgctgcg gtgtctgggg gaaacgcatc aggcgctgaa cgggctgaaa
    38221 atgattggac tcaccgcgcg ctatatggcg ttctgcatct cttctgaggc ggcggggctg
    38281 gcggaggcgg aggccaactt caaaaatctg gcagccgaaa tcagccgggt cgtcgacgac
    38341 ctcgataaca agacccgcgc gatgaaggat gctatcgaac atgggcaaga gctccttgga
    38401 ctgctgctga aagggcatac ctatgcaaag taaggtgttg ttcaataaag acgatcatca
    38461 gtgggtcgtc atcggccgtg accccgaaaa ggatgaccac gtcatcgaca ccaacgaata
    38521 tgtaatcatc catcgcggcc atgccatgct gctggacccg ggaggcattc agatttttcc
    38581 ccaggttctg gccgaattag cgaagtatgt ccgcatgcag gatatcaagg tgatctttgc
    38641 cagccaccag gacccggaca tctgctcctc gctcgccatg tggctcgacc tgaatccggc
    38701 gatcaagacc tattgctcct ggctctggac cggctttgtc agccatttca gcaccggcgc
    38761 cgtcatcacg ttaaacccga ttcccgacga cggcatgcgc atcaagatcg gcgaggaagg
    38821 tcctgaaatg gaagccgttc cagcccatta ttgccactcc tccggcaact tctctctcta
    38881 cgaccccagt gcaggcatct tgttctcggg cgacatcggc ggcgcccttg tcccggacca
    38941 ccatgccagc ctgcttgtga cggatttcga gcagcacatc cagtacatgc gggggttcca
    39001 tctccgctgg atgccctcta ccacggcgct ccgtggatgg gtccaacggg tgcgtgccat
    39061 caagcccaag atgatctgcc cacagcatgg atcgattttc gaaggagaga acgtcggcaa
    39121 actgctcgat tggctggaaa gcctggaagt cggccagtgg aaagggtcgg ccccgaagag
    39181 cgagacccgc gccgccgcct aacgccggca ccccggcgag ccaatcgatt tcgatctccg
    39241 tccgcacaga cccgcgacct tgacaacggg caaaagcggg tgctaccacc ctcccatggt
    39301 gcgggcagaa caatctccga taaccgaacc ggcggcgaaa gccgtctcaa cctggtccgg
    39361 ccaggctcgt gaacaggccg tccagcgcat gttcaccgcg atcgcgggcg tctacgacct
    39421 caacaacacc ctgctcagct tcggtctcca tcaccattgg aagcgcctca cggcttcgta
    39481 tgtgccgacc gtaacgaacg gccgtgcact cgacatcggc gccggaacgg cggatctcgc
    39541 cctgctgatc gaacccaaaa tgggcgaaca gggccatgtg gtggcctccg acttaaatca
    39601 tgccatgtta gccgaagggc tccgcaaagt gtcggggaga gggctcctcg atcgcattac
    39661 ctgcctgcag gccaatgcgg aacagctggg ctttcccgat aatacgttcc atgcagtgac
    39721 gaccggcttc tgcatgcgca atgtgggtaa cctgacccag gcgttcacgg aaatccggcg
    39781 ggttcttcga cctggcggcc gtttcgtgtg tctggagttt tcccggccgg cctatggctg
    39841 gttacgtgcg ctctatgatt ggtattcgtt tcgcctgctc ccgtggatcg gcaccagggt
    39901 ggcgcgtgat cggaccggcg tctatgaata cctgccggcc tcgatcagga ctttccccga
    39961 tcaagagcga ttggccacac tgctgaggga ggctggtttc cgcgaagtgg aataccggaa
    40021 cctcaccgga ggcatcgtcg ccatccacgt agcaaccaaa taataaccac gcgatcagct
    40081 ttcggctctc agcacaggct gatggctacg agctgacggc tgacagctga tgaattccat
    40141 cctctacatt tttctcccct gcaaaaaggt ctacccgatc ggcgtgacct acctggcaga
    40201 ctttatccat cgccggaaac ccgaggtgcg acagcgcatt ctcgatctgt cgctgttttc
    40261 cgaatcccaa cggacgcaag ccatccgcga tgccgcgctg gagttcaagc cggatctggt
    40321 ctgtttttcg tggcgggaca ttcagatttt ctccccccac gaaggagacg cctcgctgga
    40381 gcacgccttc aatttttact tcgccagcaa tccgatcaag cgggtggccg cgtcattcgc
    40441 cggcttgaag cagctctacc ggtattacag ccacattcgc gcgaacctgt cgtatccctg
    40501 gttgatccgg aaggaatttc ccaagaccca gatcatgatc ggcggcggag cgttcacggc
    40561 gtttgccgat caattgatcg agaagctccc ggaagggact attggaatcc tcggcgaggg
    40621 ggaagacgca atcctcaagg tggtcaacgg cgaatcgctt gaaaacgaac gctatatcat
    40681 tcgggaaggg aaacagacgc gcaagggaaa tcagggctcc ccggccctgc tggacgcatt
    40741 gaccgtcgat ctgccctacc tcacgtctat ttttccccag cacgcctcgt acatggacga
    40801 atcgatcggc gtccagacga aacgcggctg cccctatgat tgtgctttct gcctctatcc
    40861 ctacatcgag gggaaacggg tgcggtatcg gccgccagaa atggtcgtca aagatatctc
    40921 tcagcactat catcagtggg gcgcccgtcg attctggttc accgatgccc agttcatcac
    40981 gggcaaggaa gcctatcctc aatgcacaga gattcttgaa cgaatcctga gcgaaaagct
    41041 ggagattgag tggtcgggct atattcgcac ctcgctgatc actccggagc tggctaagct
    41101 gatggtgcga tcgggcgtcg gagacttgga agtcgccatt acctccggat cgcaggaggt
    41161 gctcaacaat ctccatatgg gcttcaaact cgaacgtctc tatgacggat gccggtatct
    41221 ggccgaagcc ggtttcaaag ggaaagtgat cctgaattac tcgctgaact ctccgaagga
    41281 gacggaagaa agtctgcttc aaagcgtgga gtcctacaaa aaagttgcgt caattttagg
    41341 tgaagagcgt gtgttcccgc tgatgttctt cctcggtatc cagcccaata cggatctgga
    41401 acaccggctg ctggaagaag gctacctttc agccggatat aaccccctca tgttgacgcc
    41461 gaccagcatt cgtaaactgc tttacaaccc ggccccgctc aacaaaatca tcgccaaagc
    41521 ttgcctgacc gcctgggaac agaaagaggg cagccgcgat cctcgcacat ggaccggatc
    41581 cctttctcaa acaccccccg ccggagcgaa gccggaccag gctcattatg ccgatgcgaa
    41641 tctcattcgg gggattcaga ataactcggg acgtgaagcg ctcttgacgt tggaagagat
    41701 ccttcgatca cgccgccccg cgcaaccaac agccgccgca acggaaaaga cggctgtgag
    41761 tccaacgagt tagcgactat ccacagaccc tttgaccacc gtcggtttgc ttgcaatccc
    41821 cgagaacacg gtgctatcat gccgcttctt ttggggtaaa gttccattca accgtttgaa
    41881 ttcttggcgg ttctctgatc tcatccccct atagagtgtt tgcctaggag gcgtttcgat
    41941 gtacaagacg atctatatcc cggtcgataa ttccgaccat tccaatacag ccgtcgatgt
    42001 cggagttgag ctggcgaaaa cctatggctc aaagatcgtg ggtagccatg tcta
//
LOCUS       DAOQEW010000009        41098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_183_length_41098_cov_8.038521, whole genome shotgun sequence.
ACCESSION   DAOQEW010000009 DAOQEW010000000
VERSION     DAOQEW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 41098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_41098_cov_8.038521"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLS35_02000"
     CDS             <1..323
                     /locus_tag="PLS35_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17450.1"
                     /translation="GIMAEEEEVQRRQEALQSLMSMRERLLRESLEARIKRARGTGDW
                     TNLSPAECASIYKEERVHLRAQLERLKAERDRTRGKLSALKRAKVRAQRIRAAEAASG
                     KKRK"
     gene            392..1201
                     /locus_tag="PLS35_02005"
     CDS             392..1201
                     /locus_tag="PLS35_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNI17451.1"
                     /translation="MTFSSRTVSRNFLSHIRDVAKTKRTRDRERVFVLEGTKPILELV
                     RSAPRQIVCLVVAPTFLERQSPGDVQEIVRSGCTVHTCPEHQLAQLSDVETSSGALAI
                     VHQPTWDQAAMLAQPRVFGLYGDMLQDPANIGTIIRTAAGLDVSALWMAPHSVDVFNP
                     KVVRATAGTLFQLPIFSHTSFENLLSLGCTILAADVGAHSNSVPIRSIQSIPARTIVA
                     IGSESRGLSDAVLDAAAMRFTIPLKPGVESLNAATAAAIALFHLSGLPVES"
     gene            complement(1210..1671)
                     /locus_tag="PLS35_02010"
     CDS             complement(1210..1671)
                     /locus_tag="PLS35_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17452.1"
                     /translation="MTDLIPPTTSRVADRTDADINARIRRQTEANVAYFAKGGAQAIS
                     ERLRELDREWDVERLLEANAATIALTGLCLGSLVNRRWFLLPAVVGGFLLQHAVQGWC
                     PPIPIFRRLGMRTQTEIEQERYALKAMRGDFANIPRDGDDRTAAVLNAVRA"
     gene            1849..3210
                     /gene="radA"
                     /locus_tag="PLS35_02015"
     CDS             1849..3210
                     /gene="radA"
                     /locus_tag="PLS35_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNI17453.1"
                     /translation="MKAKTTFHCQACGHQAPRWLGRCPDCGGWNTLKEERLPAAPKGR
                     QAMPLTAMAVATPISDIEIVGEPRHSTGMGEFDRVLGGGVVPGSVMLIGGDPGIGKTT
                     LLLQALPLLAETGEQVLYVSGEESPRQIKMRGERLGIGGKHLLILGETSLEQILKAIQ
                     EIKPAAVVVDSIQTVYTEQLTSAPGSISQVQEVAGQLMWFAKRNNVPVFIIGHVTKEG
                     AIAGPRLLEHIVDTVLYFEGDKSHSFRILRAVKNRFGSTNEIGVFEMKDGGLEEVSNP
                     SELFLAERPQRSTGSVVVSSLEGTRPILVELQALVSGTNYPMPKRMANGVEPNRLSLL
                     LAVMEKRLGMHLSGQDVYVNVVGGIHIDEPAIDLGIVAAVTSSLRESPIDFTTLVMGE
                     VGLGGEVRAISQAEMRIREAAKMGFKRCLLPERNVAKLDPVDGIELVGIREVGDALDA
                     VLA"
     gene            3216..>3472
                     /locus_tag="PLS35_02020"
     CDS             3216..>3472
                     /locus_tag="PLS35_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17454.1"
                     /translation="MATKTVRVNQVRQLLSILLLWVFTLPGCALFGPKDTTPLKEATA
                     EQLTALLKEQAGEVRTIKGLFSAKITGGILPIGQRVQGTVF"
     assembly_gap    3473..3572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3573..4231
                     /locus_tag="PLS35_02025"
     CDS             <3573..4231
                     /locus_tag="PLS35_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17455.1"
                     /translation="ITGGILPIGQRVQGTVFYQRPNAIRLRGFSAVGSEIFEFVQLED
                     QFKLRLPTMGREVTGRSSEADQLGQLTRPFQLSVWAMSGVTGTQAVGPQEQVRLSEDG
                     SRYRLDVLTAPGPSGAAPYISRRIWFDRRNLLVVQEERLTPAGEIEATMQLDDYRAVG
                     PLSPATLASNGQSPETVTRIMRPFTIRMTDGQGSGSLQVTFHELVPNEPIKPGELGRV
                     "
     gene            4228..5052
                     /gene="tsaB"
                     /locus_tag="PLS35_02030"
     CDS             4228..5052
                     /gene="tsaB"
                     /locus_tag="PLS35_02030"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNI17456.1"
                     /translation="MKVQPDHHLLAIDTATAWQSVALLSGEKVLALVEQDADGSHARS
                     LLGAIDRVLRETSITLKQLQSLAVSIGPGSFTGLRVGLATMLGFRAVLGLPIAPVPTL
                     EAMAWNLRDAKGLLVPVLKSRHNEVYWAAYEWLPESGLRTLIAEQVGPPGSVARALQG
                     SNSCTVFGDGWQAYENDIRNAIEAVGGQVQEVSEETHRPSAVSVGLAGQRRLDGGQIA
                     EQELVPRYVQRTEAEVKFDEQQGVSALERRRQRVASKLTQRRARPVPKGSDVRRRT"
     gene            5080..5586
                     /gene="rimI"
                     /locus_tag="PLS35_02035"
     CDS             5080..5586
                     /gene="rimI"
                     /locus_tag="PLS35_02035"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNI17457.1"
                     /translation="MTSTSILIEPATPAVLDEVLAIEQACFSAPWTRKMLAAELSGNQ
                     FANFLIAKCPDPLSGSFAVAGYFCFWVVFEEVRLMNLAVLEPFRRQGVATKLVCTALQ
                     TGVERGASRAMLEVRESNLEAQTLYRRLGFRQTAKRSRYYNHPEEDAVLMEMTPLQVS
                     EVCRQPGV"
     gene            5651..5866
                     /locus_tag="PLS35_02040"
     CDS             5651..5866
                     /locus_tag="PLS35_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HNI17458.1"
                     /translation="MQTETAITERLRRSNTEFRALEESHHRLDEKLADLQRRHVLTPA
                     EEVLKKQLQKEKLATKDKIAELIRSSR"
     gene            5961..6731
                     /gene="tatC"
                     /locus_tag="PLS35_02045"
     CDS             5961..6731
                     /gene="tatC"
                     /locus_tag="PLS35_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNI17459.1"
                     /translation="MLAPLAAHIQSLKKRLLIIAVTLGVAFASAFAYSTEMVAWLNRP
                     FPNQLVFYGPTEALFASIKVSFLAGIILSLPIVFYQFWKFIEPALLPKEQRWAIPIFL
                     LAALLFALGLIFCNLVILPLVIDFFVSFGMDRDITPALGVGTYIDFNVKFLLIFGCAF
                     ELPLVLTILSRVGVVSAAVLAHYRKHAIMAALIISAIVTPDATLFTMLLMAVPLMVLY
                     EIGIIGAKIFGRSSGPAPDMNLPLDPDIPIGTAGHRVR"
     gene            6766..7842
                     /locus_tag="PLS35_02050"
     CDS             6766..7842
                     /locus_tag="PLS35_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17460.1"
                     /translation="MLRHMLVLTALTVGGLQGLVHAAPSLIGNEGGGALKLEQGHPGQ
                     DPSSPADIGNTSIQALASGAGGVIYAGSFGMGIFRSTDRGGTWMSANEGLTDPFVLCL
                     TTAKDGAIYAGTFRGGVFRSRDAGKTWQPINKGLKRLEIKVLMMDAKGLYAGTGDGVY
                     LLNAAQDHWKVVTTGLDEILVHALARTDDGTLYAGTSGKGVHSYKERTPGWTRLRQGL
                     KDHEGLVENFIRVLAVDKEQGVYAGTFDGGVFRSGDGGKTWLPISRALPNDSIRGLIS
                     NERGVYVATGRGIFKTVDKGRQWMPLNKGLSNLSVQVLIEGDKGGFYAGTSSGVFRSD
                     DDGLTWTAVSQGLEGESVAPFKFN"
     gene            7860..8369
                     /locus_tag="PLS35_02055"
     CDS             7860..8369
                     /locus_tag="PLS35_02055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNI17461.1"
                     /translation="MTAQTGSPQATITVTSKNEPWGQIVLRFFPDVAPNHVKNFTDLA
                     KKGFYNGTTFHRVIPGFMIQGGDPNSKNPDRASHGMGGPGHNVKAEFNSKPHKRGTLS
                     MARANDPDSAGSQFFICVNDANFLDWQYTVFGEVQSGLDVVDKVVGAKRDGRDNPLER
                     VEMTVTITE"
     gene            8408..9112
                     /locus_tag="PLS35_02060"
     CDS             8408..9112
                     /locus_tag="PLS35_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17462.1"
                     /translation="MTPVLRFLRVTVHLSQVAVLAAAVFTAAGCAIPQVPSRTVYEDP
                     VNFVRLELDANVLPEWPPGHFTHPARFSQEQVRRVLMGLTVQEHRTMVQRWIGDDSRR
                     LPMFRDAEIMILVPQLVEALRLARENERVTYYLSQPQTSVKRTITSGGMYIRGTELHF
                     ILGNWQTLYGIPAYGMIYDRRYPMNPIISKGFDLFFDLDQALVTQTTSIWDGLLANTK
                     DELVIDLAIVFPGQNI"
     gene            complement(9167..10606)
                     /locus_tag="PLS35_02065"
     CDS             complement(9167..10606)
                     /locus_tag="PLS35_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI17463.1"
                     /translation="MTYLLLRSTSLMLAALSLAAASASGTYAAEAEQPVPLSRGSFLG
                     VQQAVEIAVKNHPMLIEGQANLKASEARTEQARSLYYPQVYANANTVAGAGVSNPRFL
                     IGGGLLRENQTTFTGGVIANQRLYDFGYTSNLIESNKLAERAQGQDVNARRALVLLYV
                     QRAYLNSLKRHRLVQIAEETVRERGIIAGQIETLYRQQLKSKLDFDLARVELVNAQSL
                     LVRSRNDLKASFADLNRTMGIAGADDYVLEDISVAVRPQKTLETLINESLSHPEVKRA
                     KEQTASADARLTATKRQYLPTVSAIASGGTFDPFDPRQNQQTGGWWMAGAMVSMPLFT
                     GFLIENQVVEANANRQAASAATSNIEQALTQQVTNAYLDTLTFAQQITLAEEQVKTAQ
                     EALQLSKQRYKLGLGTVVEVTQSEVAVTAAQTRLADTQYDYKIAEVTLAYTSQGDDTG
                     RILLMSRASTASRPGDAGSQAEGPPAAAY"
     gene            complement(10603..11907)
                     /locus_tag="PLS35_02070"
     CDS             complement(10603..11907)
                     /locus_tag="PLS35_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI17464.1"
                     /translation="MDEPSTTLRPVRATRRKIRWIALAIVLTLVIGGLLYWREIHDVG
                     HPVPPASPAVPTPPQPPEESVAVPDSAPVDVQVTKPTRRDLVYTVKLPANVSPLYQTT
                     LYAKVSGYLKWIGPDKGDAVKRNDVVAVIDAPEVEEQYQQAVSDYKIKKLTYERLAKV
                     WKESPDVIAKQDVDVAEAAYQGARHLMEQRVVMRDYTKVRAPYDGIVTARFADPGALI
                     QIATSSATSAIPLFTIMDLNTVRIYANVPQDDSPWIVPGKTQATVKVTELPSRSFTGL
                     VTRSTMALDPATRSLLVEIDLPNQDHALRPGTFAEVTLGLREIPQALVVPPQAITSTP
                     KGKSVFVVEDGKAKPVAVQTGISDGRWIEISSGLRGDEDVVAVGKRRLLEGMPVVASP
                     FNLPDAKLSQQKFERRAPGGTPPPPTAAPPTNRAAQTQGDRP"
     gene            complement(11907..15176)
                     /locus_tag="PLS35_02075"
     CDS             complement(11907..15176)
                     /locus_tag="PLS35_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI17465.1"
                     /translation="MLTRAALKNPYAVFALCMIALILGGVSYQKMRVDIFPEIKLPSI
                     LVTTFYRGLSPSEMEGAITLKMEQRFVEASYVEHIESQSLAGMSYIKVFFQPDYGIDA
                     AQSELTSLAYSIIRLLPPGVYPPSVYKFGVSSLPVGYLSISSDALGPKEIRDLAYFTV
                     RQQIANIPGISFGPPLGGKVRQITVFLDQQRLLARGISPSDVVNALNQQSAIIPAGDI
                     KIGDLDYYVYSNSLVDVVDKINDLPIKIVNGTPVLIRDIGTAADSAAVQTSIVRVNGR
                     EATYIPITRQEGANTLEVTDGIRAKLSKLTEIPAGTTVKFLYDQSLYIRQSIANLQKE
                     GLLGAGLAGLMIFIFLASVKAALVVGLAIPLSLTVALVALYLTGQSVNIMTLGGLALV
                     IGTLLDNNIVVQENLHRHLEMGKDGRSAAEDSAMELTLPILVATICILIVYLPIMFFT
                     GIIKYLFVPLAMTVAFAMLADYVVSMSVTPVVLAWLYQGGHGKGTEHEASADEGWFRY
                     VLAIYEPILKGGLRFKPVVIGLATVALIATGAFLIPRLHTEFFPKVDAGNFTMLVTAP
                     EGSRIEKTTAIVGQVEQLVHETIPKADLEEVISNTGLYYGDAARFAPNTGNHTAFVLV
                     NLITGHEGRTEDYIAQLRSRLKTSLPGVEISFQTGGIISDVLNFGLKAPIDIQVKGPS
                     LDVIRPVAERIQQQIAQVPNTVDVRIKQGKSYPELHIDVDRTKAAYYGINQNRVVVDV
                     ITGISSNLALSPNYWLDPKTANGYFLLAQYPEQSLTTTEDLLNIPIIGARTPLLPTSS
                     LTGGGIQGSTLALQNTPFAGRQMEIASGYYASSDDRRGPPVMLRDVAQLKFKTGPDSV
                     DHYDLSRLINVLVTPVGNDLGHVAKDIEQVLAGIQLPKDVTVQLRGEVANMRGAIQNF
                     ALALPLAVVLIYLVMVALFRSFIDPLIILVAVPLGWIGTVLTLHMTNTSVNVESMIGT
                     LMMMGIVVSNSILLVDFANRMVRNGSTAAHAVLEAGRRRIRPILMTALATILGLLPLA
                     LGFGEGNETMVPLARAVVGGLAVSTIMTLLVVPVMHSLVLIRRERAPLSATAPATSED
                     I"
     gene            complement(15170..15604)
                     /locus_tag="PLS35_02080"
     CDS             complement(15170..15604)
                     /locus_tag="PLS35_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17466.1"
                     /translation="MPSSCSILLIDDSPGECELFRQALTQTGYKGRLDIVEGGQAAMA
                     YLHEHVQHEEPALILLDLKLRGERGVDVLKRIKQEARVAHIPVVILTSSDDTADVRAC
                     YSGGANGYVVKPGQFDDLVTLSLHLWKFWLEHNCTQRMAAPC"
     gene            15958..17220
                     /locus_tag="PLS35_02085"
     CDS             15958..17220
                     /locus_tag="PLS35_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI17467.1"
                     /translation="MSDDHSTPDSSRGWRLICTRDFGLLWWGQTTSQIGEGLNKVALL
                     WFVYELTGSAMKMTMVGLLQTIPPLVLGPLVGVYLDRMPKKPVMIWVDILRALLTLLI
                     PTLYALDMLSITGLYGIIFLTSVVSTIFGPALVSAVPLLVRPSELMSANALIQGTNNI
                     GMLLGPAISGIMIALIRAENVLFVNSATFLISALCLMPIRFSPSHAVSSEQATSVWQE
                     LQVGFRFIFGQQSMVLTLVIISSLYNLGVSAFVFILPVYAKEFLQVGPVQLGWLWSAL
                     GVGMLAASAWLALKQESDIQGRLRIVVSGMTIGGLAVCSLSLLETPLIAAGIVIIVGG
                     STAVLNPIIWALLQELTPEHLIGRVVTTFSVGSMASAMAGMTGFGWVADAVGPAASLV
                     ALGLVLLLTAAVAVACVRRTCALPVAHA"
     gene            17315..18634
                     /locus_tag="PLS35_02090"
     CDS             17315..18634
                     /locus_tag="PLS35_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI17468.1"
                     /translation="MGHLSPFSLADSPPAANVYTTNMSDTHRWLLTRDFGFMWWSQVL
                     SQVAEGVSKLALLWFVYAVTGSPLKTTVIGLLQTLPPILFGPFIGVLVDRFPKKTILI
                     TSDVARGLLIGLIPCLVPIEHFTVESLYVLTFLFGMATAVFVPTLSSAVPFMVARPQF
                     TAANALLQSTTSLGIIIGPAVSGLGIAFSGSQDVLCVNALTYFASAACLLPIRLRATE
                     QAPDSSAGWRPFIRHLLEGLRYAFLTHRTLLILIVLASIYTFGSGAFTTLFPVFGRQL
                     LGLGPVEVGYLWSWLGVGLLLSSIGLIWLSAWDLHKRLLAIAVSCALAGLAVVGLTWT
                     DSVGVASAYVVCIGIGLGTWTPIAWGIIQEVAPPGMVGRVMAVYTAMATATSMLGMSA
                     FGWVAESFGSITSVLGIGGVMGVLAWGSAWFGGRIRTVGVTEGGNRS"
     gene            19025..19777
                     /locus_tag="PLS35_02095"
     CDS             19025..19777
                     /locus_tag="PLS35_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17469.1"
                     /translation="MGATEELAKKFQTPLTDVVSVPFQYNTFFGVGPGNATSHLLNIS
                     PVVPVRLGDWHVVTRTVIPVISVSRFTPGLEDEIPGTGGGTATGLGDINFTPFLTPGR
                     TGAVSWGLGPSITLPTSTMEELGSKKWSAGPSAVVMFRFSPVVGGVLLRQQWSFAGGR
                     HHPGVSQFLMQPFASYNLSGGWFLTSAPIMTANWEAQPGNKWLVPVGGGVGRAFILDH
                     QPMSLTAQAYYHVIRPDLGAEWQTRLTMQFLF"
     gene            19994..20497
                     /locus_tag="PLS35_02100"
     CDS             19994..20497
                     /locus_tag="PLS35_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17470.1"
                     /translation="MSKFLIQAIVVLALVGPGGQFVRADEGSSKSHHQHLQGVVIEKG
                     GALAVKVPNGSTYQLNENRSERHGHALPKAGDEVTVVIDENNNVLEVHPKGTEGKHRF
                     ITGEVVSIGVMQREITLKTSKGEQKFAMEKQELSSSIKDGARVRAELNEAGSIIDVHP
                     EKSGGGH"
     gene            complement(20573..21094)
                     /locus_tag="PLS35_02105"
     CDS             complement(20573..21094)
                     /locus_tag="PLS35_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17471.1"
                     /translation="MHKSLRHAAWIIAIAMLPGCVGLGAWTLGTRSESSDHPKIDQTR
                     GAIDLRKAGTPSEFKTVAELRTQWGEPDNIELRKDGREEWIYKTGGLRWSGMVLYLVV
                     IPLPAMIPVGSQYVSLVIHDGRIEQATRRDWAFKAGAYCGFFGMVYGGLGCGTGTFEE
                     KQGSDAQVVNGTP"
     gene            21274..21654
                     /locus_tag="PLS35_02110"
     CDS             21274..21654
                     /locus_tag="PLS35_02110"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17472.1"
                     /translation="MAPRRYARTYYRFPLNYPVIFGGAPFVGEGVLTNLSLKGCSVTC
                     DREVLCGSQVRVSMLLHHQPPALPVELGTIKWVRGNQFGVEFVRVPLEAQQRLNRTLR
                     MELIEWLENRHRNSASPASPRHDN"
     gene            21846..25073
                     /locus_tag="PLS35_02115"
     CDS             21846..25073
                     /locus_tag="PLS35_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17473.1"
                     /translation="MASDPPPSASSSQDGIQQGGHSWWARLAEWNPSLLTTVTLAVAT
                     GLRMALTPLWGSGYTFITYYPAIMFIAVANGWRHGVAATLVSSVLTIVLFFDMQASPG
                     EQQTALALFITANVIIVSLSEAVTRARLRAQAETSLVRAQEQRLRLEIDARAKAEETV
                     RASERQMQFVTDHAPVLIAQCGTDGRYRFVNQRYADLVRRTPEDLIGLHPREVLGAEA
                     YQQAEPYMREALAGRQVKFDQQFLGAASIGRLLQVTYAPEWDERGIVTGFIAAIVDIT
                     ERKQAEQARATLAAIVESSEDAIVSKDLNGLITSWNRGAERLYGYTRQEAVGQSVLLV
                     IPDEQIEEETPILAKIVRGETVQQYETIRRRKDGTLIDIALTVSAIRNDEGRIVGVSK
                     VAHDITERKRAEAALRESEERLRLFIEHAPAAIAMFDRQMRYVAVSRRWLADYGLEGE
                     VLGRSHYEIFPDIPERWRRAHQEGLAGVVMRIEEDRFERSDGTVYWLRWEVRPWRDKA
                     GGIGGIIIFAEDITDSKKAEDALRESEAQHRATFDNAAVGIAHVGLDGRWLRCNDALC
                     ALTGYSREELLARTFTEITHPDDIEGDWASVRRLLAGEIETFSMEKRYIRKDGSLIWG
                     HLTVSLLRDEQGSAHHFISIIQDIQIRKEAQAQLRQLADDLEQRVEVRTRELADTQDK
                     LRALTTELNLTEHRIRKQLATDLHDYLAQLLVLSRIKLGQAKLEEVTPAAMEAMTEVQ
                     EVMDRALAYTRTLVAQLSPPILNEFGLLMALRWLADQMQRELTVKLDLESDVLSLSEE
                     QSVLLFQSVRELLMNIVKHAGTTEAKISVTQADGVLRICVSDRGVGCDVGPGEAKLEP
                     GFGLFSIRERMKALGGEFDMQSVPGEGTTAILQLPVVQPGPTADEAVAETGVAAVSHA
                     VVSEPAAPGVPKLPTTASLLATPKIRVLLVDDHRMLRQGLRTIVNGHPGLEVVGEARD
                     GLEAIELTRTLSPAVVVMDVNMPRCDGVVATKRIKEEYPEIKIVALSMHNSPDIVARM
                     KQAGAYGYLTKESAGVQLCRAIVEAALSRPSGASGEGRDVSCGPS"
     gene            complement(25106..25486)
                     /locus_tag="PLS35_02120"
     CDS             complement(25106..25486)
                     /locus_tag="PLS35_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17474.1"
                     /translation="MVEILVVDDNLQLRDTLRKILNKRPGFRVTGEAADGIAASQLAQ
                     TLRPTVVLMDVGMARMDGVEATRRIRALLPNTVVIGMSCHNSREVEVALRAVGANAFI
                     PKELVPEQIFDVISQQLPQDQTVQ"
     gene            25716..26981
                     /gene="pfp"
                     /locus_tag="PLS35_02125"
     CDS             25716..26981
                     /gene="pfp"
                     /locus_tag="PLS35_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HNI17475.1"
                     /translation="MAIREQRPVVGILVGGGPAPGINSVIGAATIRSILGGSDVIGIQ
                     DGFKWIMDGNTSKVRRLSIEDISRIHFSGGSCLGTARANPTQKTEHLDACLASLHTLG
                     VTRLITIGGDDTAFSALKLEQRAGGRLQVVHVPKTIDNDLDLPHGIPTFGFQTARHIG
                     VELVKSLMVDAETTSRWYFVVTMGRKAGHLALGIGKAAGATLTIIPEEFRHKPIRLQT
                     LVDILVAAFIKRLSAGRSDGVAVLAEGLVEILDQQDLNGLEDVERDQHGHVRLAEINF
                     GFVLKRAVEKELRTFGIKTTIVDKNIGYELRCADPISFDMEYTRDLGYCAAQFLLEGG
                     NAAMVSIQNGRFIPIPFGDILDPATGRTRVRMVDVESESYVIARRYMIRLSSDDLHQP
                     DDLVRLATTAGLASDDFRKRFEYLIGKDV"
     gene            26997..27950
                     /locus_tag="PLS35_02130"
     CDS             26997..27950
                     /locus_tag="PLS35_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17476.1"
                     /translation="MMSAPTPDTETTIAFENQRAVLSPWGASLRRYFIVGSSGSETDI
                     VWGYSGGGQKKGGQGDVLIPFPGRVAGGRYSFDGQLLQLERNDKEGPNAIHGFVRSLP
                     WTTHQAVAHAVTYEVSVDAADYTSRGYPFSLVVRLTYSLGIQGLSCTFSVRNVGGAAA
                     PVGVGFHPYFTVGTGLIDEAEAKIPAAGFLEFNEQLAPTGKVIAATGTEWDYRTFQRI
                     GPRRFNHCYLELERDAAGMATASLRNHKSGRTIDVVMDRAFSAVVVYTGDAIADAPRR
                     ALAIEPMTCATDAFNHPDWGLKRLLPGEVFTGSYSVRARSR"
     gene            complement(28021..29766)
                     /locus_tag="PLS35_02135"
     CDS             complement(28021..29766)
                     /locus_tag="PLS35_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17477.1"
                     /translation="MAVPKDRQVRHHGRLLSLSVMLLLTPIAGTGSGAFANDLPLRSE
                     GQIAQHAKAAWEQGTLNLALGIVEQGIQDHPRALALHQLRGDMLATSRQTEAALESYE
                     TVLASIPAALDVRWAKWSVLVRSGQVEEAVAELQRIAVIDPHNPLLHLRLARELRKLD
                     RLEESFEAYQKAVELGPDMLNWRLALARARFDVLDYEGAERDVQFVLDRVPPGSPLHL
                     SARNLLTVFFGSTERGRRFAPVMTPDVSPKQLRDWSMIRAEGYRLFEAGRFREAEPVY
                     RELLRLNPMDPLAGRHLSLILMGLGRCKDALAVSQAGSEFDPTDEEHTTTAFRMGQCM
                     VELGRWEDAYMQFKTLYDATVEFEKSTKDVQLPSGTRVLNKANLVQWLEKVRLHVPEF
                     AQQVDEDAAATAAQAKAAAPTIPPEAELAAKAIEALKPQNTLDPAASLVGRDADFAWF
                     RFVIPAKKVMRDDSPTGAHDFIPLDPGISFPGSQQDIYLVFSLVTASYDEVPLAARCF
                     KETAELAGAHTAVAQDRLIMSTNDQSGYFVLPRPATGWTAGLYRCGLFAGEETSAYTQ
                     VDEVRFRIIEPTRPS"
     gene            complement(30021..31583)
                     /locus_tag="PLS35_02140"
     CDS             complement(30021..31583)
                     /locus_tag="PLS35_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI17478.1"
                     /translation="MPLSTTVHDLRTLIRSSHPLIVIETVEEDRVMTLLQSVAAQERM
                     PLFEWSITRGLTRPEEGPSLSKLTATPLAALQHLNGLTVEGIFWLKDLAPHLQDAAVA
                     RQLREVSHHLSRSRATCVMTGHPITLPLDLDQIAVRFDLQLPDRDELHAMLQSVLQSL
                     RTRTSPRRPTSTTVVQSLLNSMATPKPTMAGPSDQDCEAILRALQGLTLHQARQVITQ
                     CVIEDGVLSADDVQTILTRKVQAIRDGGLLEYYPLEDNRFELGGFVKLKSWLERAKMG
                     FTAEAKALNLTPPRGIMLVGVPGCGKSLAAKAIAREWHLPLLKLDAGRLFDKFVGESE
                     KNFRKAIEMAESLSPIVLWIDEIEKAMSAGGGSGDADGGLSRRLFGAFLTWLQEKKQD
                     VFVVATANNLSALPPELLRKGRFDEIFFVDLPDKDERTAIWSIHLNLRKQDRAQFDLP
                     KLVDASKGFSGSEIEQAVVAGLYRALYHKQPLTTDLLIQELSHTVPLSVTRSEEINQL
                     RATAQGRFVNVR"
     gene            31793..32803
                     /locus_tag="PLS35_02145"
     CDS             31793..32803
                     /locus_tag="PLS35_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNI17479.1"
                     /translation="MTPDLENSCKDEEARATPVATFWRRTGAAVADVLLLGAIGACLG
                     FLWFDQLAALGRTGRFIGAAIALVYFATLNSQIGQGQTLGKRLAKIRVTDAKGALISL
                     PRSAFRATIFFAPLALNGTATPPGPYEQLVGIVLSILIFGVGVAQAYLYCCNRRTGQA
                     LHDIVVGTFVREAESASEIQQKMWKPHLAIAGGLSTLVLGVLLLPYTSDPPDFVSQLA
                     EARRLAQSVVPDAHVSISRQTSMMTTPGSGQTPASRVQLSVEVRVKPENFESVADNIA
                     MAMFRSSSGVGDVGQVVIVISYGYDIIISHARTTKVFAHTPQEWLKRLKLDELHNVVP
                     PA"
     gene            complement(32905..33765)
                     /locus_tag="PLS35_02150"
     CDS             complement(32905..33765)
                     /locus_tag="PLS35_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNI17480.1"
                     /translation="MQHQHNTNYYAILELSADATEADIKRAWHEQMQIWHPDRFLHSS
                     TLHKKAEARTQLINQAYQTLSDPTARARYDSGRQQPSAPPPPPRPSSPPRPQPVTRPR
                     QELRGPQTMLNVTRFSHPKIMVPAIHLLVDVHETQPYAFKGLIRIAGTRTQALPAGDY
                     AIAEAPGIFCVERRRAEEFNTIVSNPSDNRPRFLRELEPLLAIPHRFLVIEGALHSHR
                     GGGRLEQYHRNGLIDFLDSITARFGLQIIYADSRDEAEERVANLAAIHYAYYLAEQQG
                     LGRCLTENDA"
     gene            33934..34953
                     /gene="galE"
                     /locus_tag="PLS35_02155"
     CDS             33934..34953
                     /gene="galE"
                     /locus_tag="PLS35_02155"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNI17481.1"
                     /translation="MILVTGGAGYIGSHTCVELLNAGSAVTVFDNFSNSHPEALARVQ
                     RITGKSLRLIRGDIRDRAALVAALRESGATSVIHFAGLKAVGESVEKPLSYYDNNVVG
                     SLRLLEAMGECGVTTLVFSSSATVYGDPQRLPLTEDHPLSATNPYGRTKLMVEEILRD
                     LQRSHHSWRIGILRYFNPVGAHASGLIGEDPQGMPNNLLPFVAQVAVGRRDHLNVWGN
                     DYSTPDGTGVRDYIHVVDLALGHLKALKALDGWDRPKECLTVNLGTGNGYSVLEIVRA
                     FEKASGKAVPYKIAQRRPGDVASCYADPGYALKALGWQAARGLDDMCTDAWCWQYNNP
                     NGYTG"
     gene            34965..36182
                     /locus_tag="PLS35_02160"
     CDS             34965..36182
                     /locus_tag="PLS35_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17482.1"
                     /translation="MMNRLTRDRAQTDLLTHRHRSVVWVLGAGALLSAFLFVPAAQAN
                     HRDHQPHADAEVPGVLGQGREIPGAPFKVFLSTGAVADVSGLDDADLDAAVQTVIDTF
                     IIMLQHRADYPRFEESLKKQALKQVVIEPVVVNDEGKAFPFLVARTKEPGRVTLLISA
                     TSLKDRGYLNHPDSLLPVLSREFQWVVSKADTAPKAKAVAVERNLEKAPILTDEEIAG
                     LSAEERVRLLQQLFDTYLRTVDDQRSLDGQPWYEVGHSAPLPPTHADSTTKLYDIRIR
                     EALQKIVRDPFFLERTPKAVRNLLNGKVWNVAYVKIDQRDWSTRTRVLSEEKSVVVGK
                     RERRIQPAAILVNVHRLAAADDPFYRETQGLPMGALSADQLAHVIALEIHHNIQEKSL
                     SGHTAQDALTAPR"
     gene            complement(36195..36863)
                     /locus_tag="PLS35_02165"
     CDS             complement(36195..36863)
                     /locus_tag="PLS35_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17483.1"
                     /translation="MDRSQAGTVRSMSFACVGLTLVSLLTGALPAAALESSARTIVSQ
                     SNSHVGLSIDLAGFTIERDQLKPNGRRYLMASHPGTGIKVAIALEQVAGKASTAGCIE
                     HLQHVQKGSAVSRGKDVTLSTAHEMPTLEYTLPRFQGVRLDQKSLYACMAEGNVYANI
                     HVSKVRYTDADASRFRQLLTTLRLQSGPSPRMTAHQSNSAAPLFSVDTVRYEQKNEAR
                     TIAP"
     gene            37373..38434
                     /locus_tag="PLS35_02170"
     CDS             37373..38434
                     /locus_tag="PLS35_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNI17484.1"
                     /translation="MGRSGMLLCAMVVMWAGLWEVGAAAEPSSVPAAEALRIMQFVLT
                     GTVSTERARGFVNEAQAAGFTAVQVLLTDGVQFDHAPWIPVKTAWTKAEFLSWADYAR
                     AHGLEVIPEVKLLTHQEQFFQRQYPGLMFNTVSYDPRKDATYAVVFPFLDELIEAVHP
                     RAIHIGHDEAFGWTVGQVSKWLKLGEVMIPADLFLRDVVRVHDYLGKKGVETWMWADM
                     VLSPAEFPGAQTRHLHGIAAGYGKALRQQLPRDIVMCDWHYGDEQGSFPSIDIMQGEG
                     FRVIGATWKQESATRKFSRYALSRHAYGLMATTGFHIQQNDTKMIHWIIQASGALFRN
                     PEAVVPPMPASAGSSEGRG"
     gene            38751..39842
                     /gene="ychF"
                     /locus_tag="PLS35_02175"
     CDS             38751..39842
                     /gene="ychF"
                     /locus_tag="PLS35_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNI17485.1"
                     /translation="MSVKCGIVGLPNVGKSTLFNALTKSGIAAENYPFCTIEPNIGIV
                     EVPDARMQALADIVKPQRMQYATTEFVDIAGLVAGASKGEGLGNQFLANIRETDGIVN
                     VVRCFEDDNVVHVAGKVDPISDIATIVTELALADLTTVEKAQERNVKLIRSGDKDAAK
                     LGDLLVQVAACLNEGKPARTMKLDPAQRALLKPLCLLTMKPVMYVANVSEKGFANNPL
                     LTRVEDYAAQEGAPVVAICAALESEIAVLSDEEKQEFLADIGMKEPGLNRLIRAAYQL
                     LGLQTYFTAGVKEVRAWTIHIGDTAPQAAGVIHGDFEKGFIRAEVIGYDDYIACKGEA
                     GAKEKGKMRLEGKEYVVRDGDVMHFRFNV"
     gene            39897..40355
                     /locus_tag="PLS35_02180"
     CDS             39897..40355
                     /locus_tag="PLS35_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNI17486.1"
                     /translation="MMNEQDTQRAVAILNRIMELELAGVVRYTHYALMIYGYNRIPIV
                     SWLKGNADESLAHAHKAGELVTLLGGHPSLKIGTLLETEKHDIGDILRESLEHEKAAV
                     ASYYELLKLAEGKSVLLEEFAREMIVGEELHLDEVNKMLRKSGDVQAFRS"
     gene            40453..40917
                     /gene="trmL"
                     /locus_tag="PLS35_02185"
     CDS             40453..40917
                     /gene="trmL"
                     /locus_tag="PLS35_02185"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNI17487.1"
                     /translation="MFDVILYQPEIPPNTGNIIRLCANTGARLHLVKPLGFTLEDKQL
                     LRAGLDYHEFATLAVYETWETCLERFTGQRLFAVSTKGRQRYDLVRYAEGDVFVFGPE
                     SRGLPAEILASVSDQQRIRVPMVPGSRSLNLSNSVAVVLYEAWRQAEFGQCL"
BASE COUNT         8185 a        11309 c        12761 g         8743 t
ORIGIN      
        1 gcggcattat ggccgaagag gaagaagtcc agcggcggca ggaagccttg cagtcgttga
       61 tgtcgatgcg cgagaggctg ctgcgtgaat ccttggaagc gcggatcaag cgggcacgtg
      121 gaacgggaga ctggaccaac ctgtctcctg ctgaatgtgc cagtatttac aaagaggaac
      181 gcgtgcacct tcgggctcaa ttggaacgat tgaaggccga gcgggaccgc acacgcggca
      241 agctgtcggc tctcaagcgg gcgaaagtgc gtgcgcaacg tatccgtgcc gcggaagccg
      301 cttcggggaa gaaacgtaaa taggatagat agcggggagg tggtgctatt ccgcactcct
      361 ccccgcagtg ccgcagacct ccaggtatct gttgaccttc tcatctcgca ccgtctctcg
      421 caactttctc tcccatatca gggatgtcgc taagacgaag cgtacgcgcg accgcgaacg
      481 cgtctttgtg ctcgaaggca caaagccgat tcttgaatta gtgcgatcgg cccctcgcca
      541 gatcgtctgt ctcgttgtcg ctccgacgtt tctcgaacgc cagtcaccgg gtgatgtgca
      601 ggagattgtc cgcagcgggt gcaccgtgca cacgtgcccg gaacatcagc tggcccagtt
      661 gtctgatgtc gaaacctcga gcggtgctct cgccattgtg catcaaccga cctgggatca
      721 ggctgccatg ctggcgcagc ccagagtgtt cggtctctac ggagacatgt tgcaggatcc
      781 ggcgaatatc ggaacgatta tccggacagc agcgggactc gatgtgagcg ccttatggat
      841 ggcaccccat tctgtcgatg tgttcaatcc aaaggttgtg cgggccacgg ccggcacgct
      901 gtttcaactc ccgattttct ctcataccag ctttgagaac ctgctgtctt taggatgtac
      961 gattctggca gccgatgtcg gggcccattc gaacagcgtc ccgatccggt cgattcagtc
     1021 cattcccgct cgcaccatcg tggcgatcgg cagtgaaagt cgtggcttgt cggatgccgt
     1081 ccttgacgcc gccgcgatgc gatttacgat cccgctcaaa ccaggggtcg aatcactcaa
     1141 tgccgccacg gcggccgcca tcgccctgtt tcatttgtcg ggattaccgg tagaatcata
     1201 ggccgctcat caggcgcgca cggcgttcaa gaccgctgcg gtccggtcat cgccgtcgcg
     1261 tgggatgttc gcaaaatctc cccgcatcgc tttcagggca tatcgttcct gctcgatttc
     1321 cgtctgagtc cgcattccca atcgacgaaa gatcggaatt ggtgggcacc atccttgaac
     1381 ggcatgttgg agtaaaaatc ctccgacgac ggccgggagg agaaaccagc gccggttcac
     1441 cagacttccg agacagaggc cggtcaaggc gatcgtcgcc gcgttggcct ccagcagccg
     1501 ttccacgtcc cattcgcggt ccagttcgcg taggcgttca ctgatggcct gtgctccacc
     1561 ctttgcgaaa taggcaacat tggcttctgt ttggcggcga atgcgcgcat tgatgtccgc
     1621 atcggtccgg tcagccaccc gtgacgtcgt cggcggaatc agatcggtca tcgagagcct
     1681 cctttgtttc tgtgttcggc acgtcatcca ctatgagatt cagagggcaa cgatgtggac
     1741 taggcattcc ccgcggaggc cttatatcgt ctccagatct ccttcctttt tctcgtccgt
     1801 ttctattacg atgccggtac acaggtgctg agcctgaggc gaacatcaat gaaggcgaag
     1861 acgacatttc attgtcaggc atgtggccac caagccccgc gctggctcgg acgctgcccc
     1921 gactgcgggg gctggaacac gctcaaagag gagcgcctgc ctgccgctcc caaaggtcgc
     1981 caggccatgc cgcttacggc catggcggtt gccacaccca tctctgacat cgaaatcgtc
     2041 ggcgagccgc ggcatagcac cggaatgggc gaattcgatc gggtcctcgg cggcggcgtc
     2101 gtgcccgggt ccgtgatgtt gatcggcggc gacccgggta ttggcaagac gacgttattg
     2161 ctgcaggcct tgcccctgct ggcggagaca ggcgagcagg tcttatatgt ctccggtgag
     2221 gagtctcccc ggcagatcaa gatgcgaggg gaacgcctcg gcatcggcgg caagcatctc
     2281 ctcattctcg gagagacatc cctcgaacaa atcctcaaag cgatacaaga aatcaagccg
     2341 gcagcggtcg tcgtggattc gattcagaca gtctacaccg agcaattgac ttcggcgccg
     2401 gggagtatca gtcaggtgca ggaagttgcc gggcagttga tgtggttcgc gaagcgcaac
     2461 aatgtccccg tgtttatcat cggacatgtc acgaaagaag gcgccattgc cggaccacgc
     2521 ttgctggaac atatcgtcga tacggtgctg tacttcgaag gcgacaagag ccacagtttt
     2581 agaattttgc gcgcggtgaa aaatcggttc gggtcgacga atgaaatcgg cgtattcgag
     2641 atgaaggatg gcgggctgga ggaagtcagc aacccctcgg agttgtttct cgccgaacgc
     2701 ccgcaacgca gcaccgggtc cgtcgtggtg tccagcctcg aggggacacg accaattctt
     2761 gtggaacttc aggcgctggt atcgggaacg aattatccga tgcccaagcg gatggcgaac
     2821 ggagtggaac caaatcggtt gtccttgctg ctggccgtga tggaaaaacg gttgggcatg
     2881 cacctctcgg gacaggatgt gtacgtgaat gtagtcggcg gaattcatat cgatgagccg
     2941 gccatcgacc tgggtattgt ggccgccgtg acgtcgagtt tgcgcgagag cccgattgat
     3001 ttcacgactc tggtcatggg cgaagtgggc ttgggcggcg aggtccgggc aatcagccag
     3061 gccgagatgc ggattcgcga ggcggcgaag atgggtttca agcgttgcct gttgcctgag
     3121 cggaacgtgg cgaaactcga tccggtagat gggattgaac tggtcggcat ccgtgaggtc
     3181 ggagacgcgc tggatgcggt gttggcatga ggaccgtggc tacgaaaacc gtacgcgtaa
     3241 accaagtgcg ccaattactg agtatcctcc tgctctgggt cttcacgctc cccggctgcg
     3301 cgctctttgg tccaaaagac accactccgc tcaaagaggc gacggcggag cagttgaccg
     3361 cgctcttaaa agagcaggcg ggagaagtgc gaaccatcaa aggcctcttc agtgccaaga
     3421 ttaccggcgg catcttaccc atcggccaac gagtccaggg cacggtgttt tannnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagattacc ggcggcatct tacccatcgg
     3601 ccaacgagtc cagggcacgg tgttttatca gcggcccaac gcgatcaggc ttcgtggctt
     3661 ctcggcggtg ggtagcgaaa ttttcgagtt cgtccaactg gaagatcaat tcaagctgcg
     3721 gctgccgacg atgggccgtg aagtgaccgg tcgatcttct gaagccgatc aactgggaca
     3781 gcttacgcgt ccgtttcagt tgagtgtctg ggccatgagt ggcgtgacgg ggacgcaagc
     3841 cgtcggcccg caggagcagg ttcggttgtc ggaggacggc tctcgatacc gcctggatgt
     3901 gttgacggca ccgggaccct ctggggcggc tccttacata agtcgacgaa tctggtttga
     3961 ccgtcggaat ttgctggtgg tgcaggaaga acggctgaca cccgccggcg agatcgaagc
     4021 gacgatgcaa ctcgatgact atcgggcagt cggcccgttg tcgccggcca ccttggcctc
     4081 caatggacaa tcgccagaga ccgtcacgcg catcatgcgt ccctttacca ttcgcatgac
     4141 cgatgggcag ggatccggaa gtctgcaggt gacattccat gaactggttc cgaatgagcc
     4201 gattaaaccg ggtgagctgg gacgcgtatg aaggtgcaac cggatcacca cctgttagca
     4261 atcgataccg cgacggcctg gcagagcgtg gccctgttga gcggtgagaa ggtgctggcc
     4321 ctcgttgagc aagatgccga cggatcgcac gcacggtcgt tactgggcgc cattgatcgt
     4381 gtgttgcgtg agacgagcat tacactgaag caacttcaga gtctggcggt atccatcggc
     4441 ccaggttcat tcaccggttt gcgggttggg ctcgcgacca tgttggggtt tcgtgcggta
     4501 ttgggtcttc cgattgcacc ggtccccaca ttggaagcca tggcctggaa cctgcgtgac
     4561 gcgaaagggc tcttggtgcc ggtcctgaaa agccggcaca acgaagtcta ttgggctgcc
     4621 tatgagtggt taccggaatc gggcctacgg accctcatcg ccgagcaggt tgggccgccg
     4681 ggttcggtag ctcgagcctt acaggggagc aattcctgca cagtgttcgg tgatggatgg
     4741 caggcgtatg agaacgatat caggaatgct atcgaggccg tcggcgggca agtgcaggaa
     4801 gtcagcgagg aaacacaccg tccttcggcc gtgagtgtgg ggttggcggg ccaacgccgt
     4861 ctggacgggg gacagattgc ggaacaagaa ttggttcccc gctatgttca acgcacggaa
     4921 gcggaagtga aatttgacga acagcagggc gtctccgcgc ttgagcggcg acgccagcga
     4981 gtggccagta agctgacgca gcgacgagct cgacctgttc caaaaggcag tgatgtccga
     5041 cgcaggacgt agtgtgccac atggagcctg ttctctgaga tgacatcgac gtcgatcttg
     5101 attgaacctg cgacaccagc cgtcctcgat gaggtgcttg ccattgaaca ggcttgcttc
     5161 tcagccccct ggacgcgcaa aatgttggca gccgaactct ccggcaacca atttgcgaac
     5221 tttttgatcg cgaagtgccc cgaccccttg tcgggatcct ttgcggtggc tgggtatttt
     5281 tgtttttggg tcgtgtttga ggaagtgcgg ctgatgaacc tggcggtctt ggaaccgttt
     5341 cggcggcaag gcgtcgcaac gaagttggtc tgcaccgccc tgcaaactgg agtggaacgt
     5401 ggtgcctcca gggcgatgct ggaagtgcgg gaatcgaatc tcgaggcaca gacgctttat
     5461 cgccggctag ggtttcgtca gaccgccaaa cgatcacggt attacaatca tcccgaggaa
     5521 gatgcggtgc tgatggaaat gacgccgctg caggtgagtg aggtgtgtcg gcagcccgga
     5581 gtgtgagctc cgggagccgg gtaacgcgat ggtcatccac gaacgtatgt agtcaaccag
     5641 gaggtgcgac atgcagacgg agacggcgat tactgagcgg ttacggcgat ccaacacaga
     5701 attccgcgca ctggaagaat ctcaccatcg cctcgatgag aaattagccg atctacaacg
     5761 gcggcacgtg ctgacgccgg ccgaagaagt gctgaagaaa cagctgcaga aagaaaaact
     5821 ggccacgaag gataagattg ccgaactcat tcgatcctca cggtaacgaa gatcgatagg
     5881 agttctgcgc cgggctcttc tcgcggacgg atggggcaga gcggagagac cgagcgcgga
     5941 ggcattggtt cagaccggac atgctcgcac cactggccgc ccatattcag tcgctgaaaa
     6001 agcggttgct gatcatcgcg gtcaccttgg gggtcgcgtt tgcgtcggcg tttgcctatt
     6061 caacggagat ggtcgcgtgg ctcaaccgtc cgttccccaa ccagctggtc ttctacggac
     6121 cgaccgaagc gttgtttgcc tccatcaagg tatcgtttct ggctggcatc attctcagcc
     6181 tgccgatcgt gttttaccaa ttctggaaat tcatcgaacc agcgctgctt ccaaaggagc
     6241 agcgctgggc aattccgatt ttcctgctcg cggcgctcct gttcgcgctc ggcctcatct
     6301 tttgtaatct cgtcatcctg ccgctggtca tcgacttttt tgtcagcttc ggtatggatc
     6361 gcgacatcac gcccgcgctc ggtgtcggca cctatatcga tttcaacgtc aaattcctcc
     6421 tgatcttcgg ttgtgcattt gaactgccac tggtgctgac cattttgtcc cgggtcggcg
     6481 tggtctcggc ggcggtattg gcccattacc gtaagcatgc catcatggcg gccttgatca
     6541 tttcggccat cgtgacgccg gatgccaccc tgttcaccat gttgctgatg gcggtcccgc
     6601 tcatggtact ctatgagatc gggatcatcg gcgcgaagat cttcgggcgg tcgtcaggac
     6661 cggcgcccga catgaatttg cccctcgacc cggatatacc catcggtacc gccgggcatc
     6721 gcgtccgata ggccggcgtt cacaattttt gaagggtgat ctcctatgct gcgtcacatg
     6781 ttggtgttga cggcgttgac ggtcggcggt ttacagggat tagtgcatgc cgctccctcg
     6841 ctaatcggga atgagggagg aggcgcgctg aagttagaac agggacatcc cgggcaggat
     6901 ccttcctcac cagccgatat cggcaacacg agtattcaag ccctcgcgtc aggtgccgga
     6961 ggcgtgatct acgcaggctc attcggcatg ggcattttcc ggagtaccga tcggggcggc
     7021 acctggatgt cggccaatga gggactgacc gatcccttcg tgctctgcct caccaccgcc
     7081 aaagacggcg cgatctatgc ggggacattt cgtggcggcg tgttccgctc ccgcgatgcc
     7141 gggaaaacct ggcagccgat caacaaggga ctgaaacggt tggagatcaa agtcctgatg
     7201 atggacgcca agggactgta tgcaggaacc ggcgacggtg tctacctgtt gaatgcggcg
     7261 caggatcact ggaaagtcgt gaccaccggc ctggatgaga ttctggttca tgcgctggcg
     7321 aggactgatg atggcacgct atatgccgga acctcaggca aaggggtgca tagttataaa
     7381 gaacgcacgc cgggatggac gcgcctgcga cagggcctca aagatcacga gggcttggta
     7441 gagaatttta tccgcgtgct ggccgtcgat aaggagcaag gggtgtacgc cgggacattc
     7501 gacggtggag tgttccgcag cggcgatggt ggcaaaacct ggttgccgat cagccgtgcc
     7561 ctcccgaatg actccatccg cggtttgatc agcaatgagc gcggcgtgta tgttgccacg
     7621 ggacggggga ttttcaagac cgtcgataag gggcggcaat ggatgccgtt aaataagggc
     7681 ctcagcaatc tatcggtaca agtgttgatc gagggcgata agggcggttt ttatgccggg
     7741 accagttccg gcgtgtttcg cagcgatgac gacggcctca cctggacggc agtcagccaa
     7801 gggttagaag gcgagagcgt cgcgcccttc aagtttaact gagtaggaaa ggacacgaga
     7861 tgacggcaca aaccggttca ccacaagcga ccattactgt gacatccaag aacgagccgt
     7921 gggggcagat cgtgctgcgc tttttccccg acgtagcgcc gaatcatgtg aagaacttta
     7981 cggacctggc gaagaaaggg ttttacaacg gcaccacctt tcatcgggtc attccaggct
     8041 tcatgattca aggcggcgat cccaacagca agaatccgga tcgcgcctcg cacgggatgg
     8101 gtggacccgg ccacaatgtg aaggccgaat tcaacagcaa gccgcataag cggggaacgc
     8161 tgtcgatggc gcgcgccaat gatccggaca gcgcgggttc gcagttcttc atctgcgtga
     8221 acgacgcgaa tttcttggat tggcaatata cggtgttcgg ggaagtgcag agcgggctcg
     8281 acgtcgtcga caaagtggtc ggagccaagc gtgacggacg ggacaacccg ctggagcgcg
     8341 tggaaatgac cgtgacgatt accgaataat gcgacgcacc gttgaagcgt aaggagacga
     8401 ctgcgtcatg accccggtgc ttcggttcct acgcgtcacg gttcaccttt cacaggtcgc
     8461 ggtgttggcc gcagcggtgt tcaccgctgc gggctgcgcc attcctcagg tcccgtcgcg
     8521 gaccgtctac gaagaccccg tcaatttcgt ccggttggaa ttggatgcca atgtgctgcc
     8581 ggagtggccg cccgggcatt ttacgcatcc ggcccggttt tctcaggagc aggtcaggcg
     8641 ggtgctgatg gggctcacgg ttcaggaaca ccggacgatg gtccagcgct ggatcggcga
     8701 tgactctcga cgattgccca tgttccgcga tgcggagatc atgattctcg tgcctcaact
     8761 ggtcgaggcc ctacggttag cacgagaaaa tgagcgagtg acctactacc tcagccagcc
     8821 tcaaacgtcg gtgaagcgga cgatcacctc tggagggatg tatatccggg gtaccgaact
     8881 gcactttatt ttggggaatt ggcagacgct gtatggcatt ccggcttacg ggatgattta
     8941 cgatcgacgg tacccgatga atcccatcat ctccaaagga ttcgatctgt ttttcgacct
     9001 cgaccaggcg ttagtcactc aaacgaccag catctgggat gggctcctcg ccaatacaaa
     9061 agacgaactg gtgatcgatc tcgcgattgt ctttcccggt cagaatatct gaaccgcccg
     9121 tcgctcctga aacggttcct gcagtctgtg gaagcctcac gacgccctag tatgccgctg
     9181 cgggtggtcc ttccgcctga ctccctgcat caccagggcg ggaagccgtc gatgcgcggc
     9241 tcatcagaag aatccggccg gtatcgtcgc cttgggaagt ataggccagc gtaacttctg
     9301 cgatcttata atcgtactgc gtgtccgcga gtcgcgtttg cgccgctgtg actgccactt
     9361 ctgattgcgt cacttccacg accgtgccca acccgagttt gtaccgctgt ttggacaact
     9421 gcaacgcctc ttgtgccgtc ttcacctgtt cttccgccag tgtgatctgt tgcgcgaagg
     9481 tgagagtgtc gaggtaggca ttcgtcactt gctgcgtcag cgcctgctcg atgttactgg
     9541 ttgcagcgga agcggcctgc cggttggcgt tggcttccac gacctggttt tcgatcagga
     9601 agccggtgaa cagcggcatc gagaccatgg cgccggccat ccaccagccg ccggtctgct
     9661 ggttctggcg cggatcgaat ggatcgaagg tgccgccgct ggcgatcgcc gacacggtcg
     9721 ggaggtattg gcgtttggtc gcggtgaggc gcgcatcggc ggacgccgtc tgctctttcg
     9781 cccgtttgac ttccggatga gagaggcttt cgttgatcag ggtctccagc gttttctgcg
     9841 ggcgaacggc aacggaaata tcctcaagga catagtcatc ggcgccggcg atgcccatcg
     9901 tgcggttgag gtcggcgaaa ctggctttga ggtcgttgcg gctccgcacc agcagggatt
     9961 gggcattcac gagttcgacg cgggccaggt cgaagtccaa tttggatttc agttgctggc
    10021 gataaagcgt ttcgatctgg ccggcgatga tgccacgctc gcgaaccgtt tcttccgcga
    10081 tctgcaccag acgatgccgc ttcagactat tcaggtacgc gcgctgcaca tacagcaaca
    10141 ccagcgcacg tcttgcattg acatcctgcc cctgcgcgcg ttcggcgagt ttgttcgact
    10201 cgatgaggtt gctggtatag ccgaaatcgt agaggcgttg gttggcgatc acgcctccgg
    10261 taaaggtggt ttggttctcg cgcaacaatc ctcctccgat caggaaccgt gggttgctca
    10321 cgccggcacc ggcgacggtg ttggcgttgg cgtagacctg gggatagtac agggagcgtg
    10381 cctgttccgt acgcgcctcc gaagccttca aattggcctg gccctcgatc agcatcggat
    10441 ggttcttgac ggcaatttcc accgcttgct gcacgccgag gaaactgccg cgtgacaatg
    10501 gcaccggctg ttctgcctcg gcggcatagg taccggaggc tgacgctgcc gccagggaca
    10561 gggcggccag catgagagag gtgctgcgta gcagtaaata agtcatgggc gatctccctg
    10621 tgtctgggcc gctcggtttg tagggggcgc ggcggtcggc ggtggaggag tgccgcctgg
    10681 cgcgcgccgt tcaaatttct gttgggaaag tttggcgtcc ggcagattga atggcgaggc
    10741 gacgaccggc atcccttcga gcagacgacg tttgccaacg gccaccacat cttcgtcccc
    10801 tcgcaagccg gagctgatct cgatccaccg gccgtcgctg atgccggttt gtaccgccac
    10861 cggtttcgcc ttgccatctt cgacgacgaa gacggacttg cccttcggcg tgctggtgat
    10921 agcctggggg ggaaccacga gcgcctgcgg aatttcacgc agaccgaggg tgacttcggc
    10981 gaaggtgccg ggacggagtg cgtggtcctg gttaggtaaa tcgatttcga ccagcagact
    11041 acgcgtggcc ggatcgagcg ccatggtgga gcgggtgacg agaccggtga acgagcggga
    11101 cggcagttcg gtcaccttca ccgtggcctg ggtctttccc ggcacgatcc aggggctgtc
    11161 gtcctggggc acattggcat aaatgcgcac ggtgttcaga tccatgatcg taaagagggg
    11221 aatcgcgctg gtggccgagg acgtggcgat ttgaatcaag gctccggggt ccgcgaaccg
    11281 ggccgtcacg atgccgtcgt aaggggcgcg gactttggtg taatcccgca tgacgacgcg
    11341 ctgttccatc aggtgccttg ctccctgata agccgcttcg gccacgtcca cgtcctgttt
    11401 cgcgatgacg tccggcgact ctttccagac tttcgccagc cgttcgtagg tcagcttctt
    11461 aattttgtaa tccgacaccg cctgctggta ctgctcttcc acttccggcg cgtcgatgac
    11521 cgcgacgaca tcattccgct tcacggcatc gcccttgtcc gggccaatcc atttgagata
    11581 accggacact ttggcgtaga gcgtggtttg atagagtgga gacacattcg cgggcagttt
    11641 gaccgtatag accaaatcgc gtctggttgg cttggtcacc tgcacgtcca cgggagcgct
    11701 gtccggtacc gccacggatt cctcgggcgg ttgtggcggg gtgggcactg caggagacgc
    11761 agggggaacc ggatggccga cgtcatggat ctctcgccaa tacagaagtc cgccgatgac
    11821 aagtgtcagc acgatggcga gggcgatcca gcggattttc cggcgcgtgg ccctgacggg
    11881 gcggagagtc gttgacggtt cgtccattaa atgtcctccg aggtcgcggg agccgtggca
    11941 gagagcggcg cgcgttcccg acgaatcaat accagactgt gcatcaccgg tacgaccagc
    12001 agcgtcatga tcgtactgac agccaacccg ccgaccaccg cgcgggccaa cggcaccatg
    12061 gtttcgttgc cctcgccgaa cccgagcgcc aggggcaaca gacccaggat tgtggcgagc
    12121 gccgtcatga ggatcggccg gatgcgccgc cgcccggctt ccagtacggc atgcgcagcg
    12181 gtcgagccgt tgcgcaccat gcgattggcg aaatcgacga gcagaatact gttcgacacc
    12241 acgatgccca tcatcatcag cgtgccgatc atggattcca cgttgaccga cgtgttggtc
    12301 atgtgcaggg ttaacaccgt gccgatccac ccaagcggca ctgccaccag aatgatcagg
    12361 ggatcgataa aagagcgaaa cagggcgacc atgaccagat aaatcaacac cacggcgagc
    12421 gggagcgcca gggcaaagtt ttgaatcgcc ccgcgcatgt tggccacttc gccgcgcagc
    12481 tgcacggtca cgtccttcgg caactgaatg cccgccaaca cttgctcgat atccttcgcg
    12541 acatggccga ggtcgttgcc gacgggggtg acgagcacat tgatcaaccg cgacagatcg
    12601 tagtggtcga ccgaatcggg accggtcttg aacttcagtt gagccacatc gcgaagcatg
    12661 acgggcggcc cgcgtcggtc gtcgctggat gcgtagtagc cgctcgcgat ttccatctgc
    12721 cttccggcaa acggggtatt ttggagggcg agcgtggatc cctgaatgcc gcctcccgtg
    12781 agactggagg tagggaggag aggggtgcgc gcgccgatga tcggaatgtt gagcaaatct
    12841 tcggtcgtcg tcagtgattg ctcgggatac tgcgccaaca ggaaataccc gttggcggtt
    12901 ttgggatcga gccagtagtt gggcgaaagg gccaggttcg aactgatgcc ggtgatgacg
    12961 tccacgacga cgcggttctg attgataccg taataggcgg ccttggtgcg atccacatcg
    13021 atatgcaatt ccgggtaact cttgccctgc ttgattcgca catcgacggt attgggcacc
    13081 tgggcgatct gctgttgaat ccgctcggcc accggacgga tcacgtcgag actcggacct
    13141 ttcacttgaa tgtcgatcgg ggctttcaat ccgaaattca acacatcgct gatgatgccg
    13201 ccggtctgga aagagatctc gacaccgggc agcgaggtct tgagcctgct ccggagctgg
    13261 gcaatatagt cctcggtgcg gccttcgtgt cctgtgatga ggttgacgag cacgaaggcg
    13321 gtatggttgc ccgtgttcgg ggcgaatcga gcggcatcgc cataatagag accggtgttg
    13381 gaaatcactt cttccagatc cgctttgggg atcgtctcat gcaccaattg ttcgacctgt
    13441 ccgacgatgg ccgtcgtctt ttcgatccgc gaaccttccg gcgcggtgac cagcatcgtg
    13501 aagttgcctg cgtcgacttt tgggaagaat tcggtgtgca agcggggaat caagaaggcg
    13561 ccggtggcga tcaacgcgac cgtcgccagg ccgatcacga ccggcttgaa gcgcaggccg
    13621 cccttcaaga tcggctcata gatggcgagc acatagcgga accatccttc atcggccgag
    13681 gcctcatgtt ccgtgccttt gccatgtcct ccctggtaga gccaggcgag cactaccggg
    13741 gtgaccgaca tggacacgac atagtctgcc agcatggcaa aggcgacggt catcgccagc
    13801 ggcacgaaga ggtacttgat gatgccggta aaaaacatga tgggcagata cacgatgagg
    13861 atgcagatcg tggcgacgag aataggcagc gtcagctcca tcgcgctgtc ttcggctgcc
    13921 gagcgtccgt ccttgcccat ttccaagtgt cgatgcaggt tttcctgcac gacgatgttg
    13981 ttgtcgagca gggtgccgat gacgagggcc aatccgccga gcgtcatgat gttgacgctt
    14041 tggccggtca gatacagggc aaccagcgcc accgtcagcg agagggggat ggccaatccg
    14101 accaccagcg ccgctttcac gctggcgagg aaaataaaga tcatcaaccc ggccagacca
    14161 gcgcccagca agccttcctt ctgaagattg gcaatcgact gccggatata gagcgattga
    14221 tcgtagagaa acttcacagt agtgcctgcg ggaatttcgg tgagtttgga cagtttggcg
    14281 cggatgccat cggtgacttc cagcgtattt gcgccttcct gcctggtgat gggaatgtag
    14341 gtggcttcgc ggccgttcac gcgcacgatc gaggtctgta cggcggcgct gtcggcggcc
    14401 gtgccgatat cgcggatcag aaccggggtg ccgttgacga tcttgatcgg taggtcgttg
    14461 atcttgtcca cgacgtccac aaggctattg gaatagacgt aatagtccag gtcgccgatt
    14521 ttgatgtcgc ctgcgggaat gatggcgctc tgctggttga gcgcgtttac gacatcggag
    14581 ggggagatac cgcgcgcaag caaccgctgt tggtccagga agacggtaat ttgccggacc
    14641 ttgccgccca agggcggacc gaaggaaatg ccggggatat tggcaatctg ctggcgcacg
    14701 gtgaagtagg cgagatcgcg aatttctttc ggaccgagcg catcgctgga aatagacagg
    14761 tagcccaccg gcaaactgga gacaccgaat ttataaacag agggtggata cacgccgggt
    14821 ggaagcaggc ggatgatgct ataggccagg ctggtcagtt cggattgtgc cgcgtcgatg
    14881 ccatagtccg gttgaaaaaa gactttgatg tagctcatcc ccgcgagcga ttgggattcg
    14941 atatgttcga cgtagctcgc ttcgacgaag cgttgctcca tcttcagcgt gatcgcgcct
    15001 tccatttcgc tgggactcaa gccgcgatag aacgtggtga caaggatgga gggcagcttg
    15061 atttcgggga agatgtccac acgcatcttc tggtacgaca cgccgccgag aatcagcgcg
    15121 atcatgcaca aggcgaagac ggcatacgga tttttcagcg cggcacgggt cagcatgggg
    15181 cagccatccg ttgggtgcag ttatgttcca gccaaaattt ccagagatgc agggaaaggg
    15241 tcacgaggtc atcgaattgt cccggcttga cgacgtaccc attggcgccc ccgctgtagc
    15301 aggcccgaac atccgccgtg tcgtctgagc tagtcaggat gacgacagga atgtgtgcga
    15361 cgcgcgcctc ctgcttgatg cgtttcaaca catccacgcc tcgttctcct ctgagcttga
    15421 ggtcgagcag aatcaaggcc ggctcctcgt gctgcacatg ttcatgcagg taggccatgg
    15481 ccgcctggcc cccttccaca atatccagcc gccccttgta gccggtttgc gtaagggcct
    15541 ggcgaaacaa ttcgcattcg ccggggctgt cgtcgataag gaggatggag catgagctag
    15601 gcatggcaga tcagtaacgc aggaattgtg ccgggatcat cgagtggtgt ggtgtcgcca
    15661 taagcctacg aaagcgcaag ggtctgcggg cgcacatgag aggaggttgc ttcagcgacg
    15721 gggacgacgc cgcgacaggt aggaagaaat tccagagggg tagggcaata ttcccgtgtg
    15781 cgttctgaag ccagttttga caaatgtgga ggggctgccc gggatttgtc gatctgcctc
    15841 cagggccttt cctaggccgg ttgtctggtt gaaatggcta gcatagctcc agacgttgcg
    15901 agagggcacc gaacatttcc actcccgcct tcgattgctc caggaggatt cgtcgtcatg
    15961 tcagatgatc attcgactcc ggactcgagt cgcgggtggc gtcttatctg caccagggat
    16021 tttgggttgc tgtggtgggg acaaacgacg tctcagatcg gtgaggggct caacaaggtt
    16081 gcgctgttgt ggttcgtgta tgagctgacc ggatcggcca tgaagatgac catggtcggt
    16141 ctgttgcaga cgattcctcc gctcgtgctg ggtccgctag tcggcgtcta tctggatcgc
    16201 atgcccaaaa agcctgtcat gatctgggtc gatattcttc gcgcgctgct cacactgctc
    16261 attccgacgc tctatgcgct ggacatgctg tcgattacag gactgtacgg cattattttt
    16321 ctcacgtcgg ttgtgtccac catattcggt cccgctctcg tgtcggcggt tccgttattg
    16381 gttcggcctt ctgaactcat gtcggccaat gctcttattc agggcaccaa taatatcggg
    16441 atgctgctcg gcccggcgat cagcggcatt atgatcgccc tcatcagggc agagaacgtg
    16501 ctgttcgtga attccgccac attcctgata tcggccctgt gcctcatgcc gatccgtttc
    16561 tctccttcgc atgccgtgtc cagcgagcag gctacgtccg tctggcagga actccaggtc
    16621 ggatttcgat tcatcttcgg ccagcagtcg atggtcctca cgctagtgat tatttcatcg
    16681 ctctacaatc tcggcgtgag cgcctttgtg tttattttgc cggtttacgc caaggagttt
    16741 cttcaggtcg gcccggtaca gttgggttgg ttgtggtccg ccctgggtgt cggcatgctg
    16801 gcggcctcgg cctggctggc gttgaagcaa gagagtgata tccaggggcg cttgcgcatc
    16861 gtcgtgagcg ggatgacgat cggcggcctg gcggtatgca gtttgagtct gctggaaacg
    16921 ccgctgattg cggccggaat cgtgatcatc gtgggaggca gcacggcggt gctgaatccg
    16981 atcatctggg cactcttgca ggagttaacg ccggaacatc tgatcggtcg cgtcgtgacc
    17041 accttcagcg tcggctccat ggcgtcggcg atggcgggga tgacgggctt cgggtgggtg
    17101 gccgatgccg taggccccgc cgcgagcctg gtggcactcg gcctggtgtt gctgctcacg
    17161 gcagcggtgg ctgtcgcatg cgtgcgtaga acatgcgcgt tgccggtggc gcatgcgtga
    17221 gcatgtgtga cgttcagtat cccccgtcgt tcccttctag gacatgcaat gaaccaaggc
    17281 gatcaccccg tcgccagcga aaggatcaga atgcgtggga cacctctcgc ctttcagcct
    17341 tgctgattcc cctcctgccg caaacgttta tactacgaat atgtccgata cccatcggtg
    17401 gctgctgacc cgtgacttcg gcttcatgtg gtggagccag gtgctgtctc aagtggctga
    17461 aggcgtcagc aagctggcgc tgttgtggtt cgtctatgcg gtgacggggt cgcccctcaa
    17521 aacgacggtc attgggctcc tccagacact gccgccgata ctcttcgggc cgttcatcgg
    17581 cgtcctcgtc gatcggtttc ccaagaaaac gattttgatc acgagcgatg tcgcaagagg
    17641 tctgttgatc ggcctgattc cctgtctggt gccgatcgaa catttcacgg tcgaatccct
    17701 ctacgtcctg acattcctgt tcggcatggc tacggccgta tttgtgccga cgctgtcatc
    17761 cgccgttccc ttcatggtgg ctcgcccgca gttcacggca gccaatgcgc tgctgcaaag
    17821 cacgaccagt ctgggtatca tcatcgggcc tgctgtcagc ggactcggta ttgcgttctc
    17881 gggatcgcag gatgtgctgt gtgtgaatgc gctcacctat ttcgcatccg cagcctgctt
    17941 gttgccgatc cggttgcgag cgacggagca ggcaccagac tccagtgcgg gctggagacc
    18001 gttcattcgg catctcctcg aaggcctgcg gtatgccttt ctgactcatc gcacgctgct
    18061 gatcctgatc gtcctcgcct ccatctacac gttcggcagt ggcgcattca cgacactgtt
    18121 tcccgtattt ggacgccaac tgctcggcct tggcccggtc gaggtgggat atctctggtc
    18181 ttggctcgga gtggggctgc tcctctcatc cataggcctg atctggttga gcgcctggga
    18241 tcttcacaaa cggctgttgg caattgcggt gtcctgtgca ctggcaggtc tggcggtagt
    18301 ggggctgaca tggaccgata gtgtgggcgt cgcgtcggct tatgtagtct gtattggaat
    18361 cggcctgggc acctggaccc cgatcgcctg gggcatcatt caagaagtgg ctccgcccgg
    18421 catggtcggc cgcgtgatgg ccgtgtacac agccatggcc accgcgacct ccatgctggg
    18481 gatgagcgcc ttcggctggg tagcggagtc gtttggctcg attaccagcg tcctcggaat
    18541 cggcggagtc atgggtgtgt tggcctgggg cagcgcctgg tttggaggaa ggattcgaac
    18601 cgtgggagtg acagagggag ggaaccgcag ttagcgcaag ttgccgcgat caggagacgc
    18661 ctaaccgccc gcacatcgcc catcctgtac gctcgccatt tccgcctgtc acaaagttga
    18721 accacacggc ttgccacgta cggaaaaaat agggagaatg cgccggccag tcggcacgac
    18781 atggcctgtt cgaacaatct cactcagtac aaaaaacaac ggtatgtgta gttatgaatg
    18841 agaacagtgc agagtcgtcg cgttacacgc gtgcatccgg tcctcgctgc ttaccagtcg
    18901 tctctcaccg gtgatatcac tgttccatag agacagggcg aggtctcaaa gcttcggttg
    18961 gcttcctccg tggtctgccg cgttgctcgt catgctgttc tgcgcatcat cgagttgggc
    19021 agagatggga gctactgagg aactcgcgaa aaagttccag acgcctctga cggatgtcgt
    19081 ctcagtaccg tttcagtaca acaccttctt cggtgtcggg ccgggcaacg ccacatcgca
    19141 cctgttgaac atctctcccg ttgtgccggt tcgcttgggt gattggcatg tcgtcacgcg
    19201 cacggtcata cccgtgattt cagtgtcacg gttcactccg ggtttggaag atgaaatccc
    19261 gggcacgggc ggtggcacgg cgaccggatt gggtgacatc aatttcaccc cgtttctcac
    19321 gcccggtcgc accggggccg tatcctgggg cctggggcca tccatcacgc tgccgacctc
    19381 gacgatggaa gaactcggca gtaagaagtg gagtgccggg ccatcggccg tggtgatgtt
    19441 tcgattcagt ccggtggtgg gcggtgtcct gcttcgtcag caatggtctt ttgccggagg
    19501 ccgtcatcac ccgggcgtca gccagtttct catgcagccg tttgccagct acaacttgag
    19561 cggtggctgg tttctcacct cggctcccat catgacggcg aactgggaag cgcagccggg
    19621 caataagtgg ctggtcccgg ttggcggcgg agtggggcgg gcgtttatcc tggaccacca
    19681 gcccatgagc ctcacggcgc aagcctatta tcatgtcatc aggccggatc tgggagcgga
    19741 gtggcaaacc aggctgacga tgcagtttct cttttagcct gttgttcagt cgcgcggacg
    19801 aacataagac tcgggccgag tgatgcatgc tctcgctctg taagcctgta ggaaatcttg
    19861 tttgtctgcc agggaactca agggcttctc ctagtccatt cgtctcaccg gcatcgatat
    19921 tcttttctgt aatgggcttt tcacgggggc agcgcagaac cgctgaagtg gtccatcaac
    19981 aggagggtcg aatatgtcca aatttctcat ccaggctatc gtcgtgctgg ctcttgtcgg
    20041 accgggtggt cagtttgttc gggccgatga gggctccagc aaaagccacc atcagcatct
    20101 acagggggtc gtgatcgaaa aagggggagc acttgcagtg aaagttccca atggcagcac
    20161 ctatcagctc aatgaaaacc gctccgagcg tcatgggcat gcccttccca aggcaggaga
    20221 cgaagtcacc gtggtcatcg acgagaacaa taacgtgctc gaagtgcacc cgaaaggaac
    20281 tgaaggaaaa catcggttta tcacgggcga ggtggtatcg attggcgtca tgcagcgcga
    20341 gatcacgttg aagacctcga agggcgagca aaagttcgcg atggaaaagc aggaactctc
    20401 ttcttccatc aaggatggcg cgcgcgtccg ggctgagttg aatgaagccg gcagcattat
    20461 tgacgtgcat ccggaaaaat ccggcggcgg tcactgacct ggctcagtag gcgggagacc
    20521 ttcaccatcc ctctgtccgg tggatgatcg ggcagaggga tgggccggga cttcacggag
    20581 ttccgtttac tacctgggcg tccgatccct gtttctcttc aaacgtgcca gttccacacc
    20641 ctaatccacc gtacaccatc ccgaagaatc cgcaataggc cccagccttg aaggcccaat
    20701 ctctgcgggt ggcttgttcg atgcggccat cgtgaatgac aagagagacg tattgactgc
    20761 cgacagggat catggcaggc agggggatga caacaagata cagcaccata ccggaccatc
    20821 gtaagccgcc ggtcttatag atccactctt cccgtccatc cttgcgtaat tcgatattgt
    20881 caggctcacc ccattgcgtt cgcagttcag cgaccgtctt gaactcagag ggcgtgcctg
    20941 ccttgcgcag atcgatggca cctctggtct gatcgatctt cgggtggtcg ctggattcgc
    21001 tgcgcgtgcc gagggtccag gcgcccagtc cgacgcagcc tgggagcatg gcgatcgcga
    21061 tgatccaggc tgcatggcgt aacgacttgt gcatagtgtc ctttctctgg cgtgatgggg
    21121 cgaaccctac tcaggtccct ctcgaaatac aagcagggca gacgagacgg agactgccga
    21181 taccgaagga agaccagtcg ccaacccccc tcccaaggta ggataccgcc tgaccggcga
    21241 gcctcggtag gatcccgcca cagggaggtt gtgatggcac ctcgtcggta tgctcgaacc
    21301 tattaccggt ttccgctcaa ctatccagtc atttttggcg gagcgccctt tgtcggtgaa
    21361 ggcgtcctga ccaatctctc attaaaaggc tgctcggtga cctgcgatcg cgaagtgctc
    21421 tgcgggagcc aggtgcgggt gagtatgctt cttcatcatc agccgcccgc gttgccggtt
    21481 gaactcggga cgatcaaatg ggtgcggggg aatcaattcg gcgtggagtt cgtgcgcgtg
    21541 cctcttgaag ctcagcaacg gctcaaccgg acattacgaa tggaattgat cgaatggctg
    21601 gagaaccgtc accggaatag tgcctcgcct gcatcgccac gtcacgacaa ctagcatacc
    21661 tgcttcttca gcgtccgccc cgctagcccc atctcgcttc cccgaatgtc caggatcagg
    21721 tgtggtgtct cctgaggcat tcaggtacac tacgcctctt agccgaacat cctgcgcatg
    21781 ctgcgcacga tcattctgat cgccacctag accaagagcc gattcatcgc tcgccgatgg
    21841 agcccatggc ttctgacccg cctccttcag cttcttcatc tcaggacggg atccagcagg
    21901 gtggtcactc ctggtgggcg cgtctggccg agtggaatcc ttcactgctc acgacggtca
    21961 cgctcgccgt ggcgaccggt cttcgtatgg cgctgactcc gctgtgggga tcaggctata
    22021 ccttcatcac gtactatccc gccatcatgt tcatcgccgt ggccaatggc tggcgacatg
    22081 gtgttgccgc caccctggtc tcctcagtcc tgactatcgt cctgtttttt gatatgcagg
    22141 cttcgccggg tgagcagcag acggcgctgg cgttgtttat caccgccaat gtcatcatcg
    22201 tgtcgctctc cgaagcggtc acgcgtgctc gtcttcgggc gcaggcggag acatccctgg
    22261 tgagggccca ggaacagcga ttgcgtctgg agatcgacgc gcgcgccaag gccgaggaga
    22321 cagtccgcgc cagtgagcgt caaatgcagt tcgtcacgga tcatgctccg gttctcatcg
    22381 cacaatgcgg aacggatgga cggtatcggt tcgtgaatca gcgatatgcc gacctcgtgc
    22441 gacggacgcc cgaagatctc atcggcctgc atccgcgtga ggtgttgggc gcagaggcgt
    22501 accagcaggc cgaaccctat atgcgagagg cgctggctgg acggcaggtc aagtttgatc
    22561 agcaatttct tggtgctgcg tccataggcc gccttctgca agtgacctac gcaccggagt
    22621 gggacgaacg cgggatcgtg acgggattca ttgccgccat cgtcgatatc accgaacgga
    22681 aacaggccga gcaggcacgg gccacgcttg ccgccattgt cgaatcgtca gaggacgcga
    22741 tcgtcagcaa agatctcaac ggcctcatca ccagttggaa tcgaggtgcc gagcggttgt
    22801 acgggtatac ccggcaggag gccgtggggc aatccgtgct gctggtcatc ccggacgaac
    22861 agatcgagga ggaaaccccg attcttgcga agatcgtgcg aggcgagacg gttcagcaat
    22921 atgaaacgat ccggcggcgg aaagacggga cgttgatcga tattgccctg accgtatcgg
    22981 cgatccgcaa tgatgaggga cggatcgtcg gcgtctccaa ggtcgcccac gatatcaccg
    23041 aacgcaagcg agccgaggcg gcgttgcggg aaagtgagga acggctgcgg ctcttcatcg
    23101 aacatgcgcc cgccgcaatc gccatgttcg atcggcagat gcggtatgtc gcggtgagcc
    23161 gccgctggct ggcggactac ggacttgaag gcgaggtgtt gggccgcagt cactacgaga
    23221 tttttcccga cattccggag cggtggcgaa gggcgcatca ggagggcttg gccggcgtcg
    23281 tgatgcgtat tgaagaagac cggttcgaac gttccgacgg gaccgtctat tggctgcgat
    23341 gggaagtccg gccctggcgg gacaaggccg gcggtatcgg cgggatcatt atcttcgccg
    23401 aagatatcac ggacagcaaa aaggccgagg atgccttacg ggaaagcgag gcccaacatc
    23461 gcgccacctt cgataacgcg gcggtgggga ttgcccatgt ggggctcgat ggacgctggc
    23521 tccgctgcaa tgatgccctg tgcgccctca cggggtactc tcgggaagag ttgctcgcca
    23581 ggacatttac cgaaattacc catccggatg acatcgaagg ggattgggct tccgtccgca
    23641 ggctgctggc cggtgagatc gaaaccttct ccatggaaaa acggtacatc cggaaggatg
    23701 gctcgctcat ctgggggcat ctgaccgtct cgctgttgcg ggatgagcag ggctccgcgc
    23761 accacttcat ttccatcatt caggacattc agatccggaa agaagcacaa gcgcaattgc
    23821 gccaactggc ggacgatttg gagcagcgtg tcgaggtgcg gacgagagaa ttggcggata
    23881 cgcaggacaa gctgcgcgcg ctgacgacag aactgaatct gactgagcat cgtatccgaa
    23941 aacaactcgc gaccgacttg cacgactatc tggcgcaact cctggtgttg agtcggatta
    24001 aactcgggca ggccaaattg gaagaggtga cgccggcggc gatggaggcc atgacggagg
    24061 tccaggaagt gatggatcgg gccctggcct ataccaggac gttggtggca cagctcagcc
    24121 cgccgatctt aaacgagttc ggactcctga tggcgctgag atggctggcg gaccagatgc
    24181 agcgggagct caccgtgaag ctcgatctgg agagtgacgt cctctccctt tccgaggagc
    24241 agtccgtcct gttgtttcaa tcggtccgcg aactcctgat gaatatcgtg aaacatgcgg
    24301 ggacgactga ggcgaagatt tccgtcacac aggccgacgg agtattgcgc atttgcgtgt
    24361 cggatcgcgg tgtgggttgt gacgtcggtc cgggggaggc caagctggaa ccaggctttg
    24421 gtctgttcag tattcgcgag cgcatgaagg ctcttggagg ggagttcgac atgcaatcag
    24481 tccccggcga gggcacgacg gccattctcc agctgccggt tgttcagcca ggccccacgg
    24541 ctgatgaggc ggtggctgaa acgggtgtcg ctgcggtgag tcacgccgtg gtgtccgagc
    24601 cggccgcacc gggggtgccg aaactcccca caactgcctc gctcctcgcc actcccaaga
    24661 tccgggtctt gttggtagac gaccatcgaa tgttgcgcca ggggctgcgg accatcgtga
    24721 acggacatcc cggcctcgag gtcgtcggtg aagcccgcga tggcctggaa gccatcgaat
    24781 tgacccgtac cctcagcccc gctgtcgtgg tgatggacgt caacatgccc cgatgtgacg
    24841 gcgtggtggc gaccaaacgg atcaaggaag aataccccga aataaaaatc gtggcactct
    24901 ccatgcacaa ctcgcccgat atcgtggcac gcatgaagca agcgggagcg tatgggtatc
    24961 tcaccaaaga aagcgctggt gtccagctgt gccgagcgat tgtcgaggcg gcccttagcc
    25021 ggccgtcagg ggcgagcgga gaaggtagag atgtgagttg tggcccctca tgagaagaag
    25081 ctgtagggct gcttcttcca ccgcatcatt gcaccgtctg atcctgcggc agttgctggg
    25141 agatgacgtc aaagatttgt tcgggtacca gctctttggg aatgaacgcg ttcgcgccga
    25201 cggcgcgtaa ggcgacctcg acttcccggc tgttatggca agacattcca atcacgaccg
    25261 tgttcggcag gagcgcgcgt atccggcgcg tcgcctcaac cccatccatg cgtgccatgc
    25321 ctacgtccat gaggaccact gtcgggcgga gcgtctgggc caattgcgag gcggcgatgc
    25381 cgtcggctgc ttccccggtc acacgaaagc cgggccgctt gttcaggatc tttcgtaagg
    25441 tgtctctcag ttgcagattg tcgtcgacga cgagtatctc gaccatgaag tacctccttc
    25501 gagtgaagca gaaggctgtc ggtcttaggc tagcacaggt tcgataattc gcgagtgata
    25561 ttgaagaacc cacctggtcg tccttgacgc actttctgag tgcgtgtagg gtcgcaggca
    25621 tgaacggcct gcgggatagc cggaaacagc ctagcatggt gcgctgacgg cgcagggagc
    25681 gggtcgaatc atttcagacc gataggagca ggaacatggc gatacgtgaa cagcgaccgg
    25741 tcgtgggcat tcttgtcggt ggggggccgg caccgggcat caacagtgtg atcggggcgg
    25801 cgacgatacg aagcattctc ggcggctccg acgtcattgg cattcaggac ggtttcaaat
    25861 ggatcatgga cgggaacacc agcaaggtca gacgtctgtc gatcgaagac atcagtcgca
    25921 ttcacttcag cggaggttcc tgtctcggga cggcgcgtgc caatcctacg cagaaaaccg
    25981 agcacctcga tgcctgcctg gcaagtctgc atacgctggg cgtgaccaga ctgattacga
    26041 tcggcggaga tgatacggca ttctccgcct tgaagttgga gcagagggcc ggcggccgcc
    26101 ttcaggtggt gcacgtgccc aagaccatcg acaacgatct cgatctgccg catggcattc
    26161 cgaccttcgg attccagacg gctcgccata tcggggtcga actggtcaag agtctgatgg
    26221 tcgatgcgga gaccacctcc cgctggtatt tcgttgtcac catgggccgc aaggccgggc
    26281 acctggcgct ggggatcggc aaggctgccg gagccaccct gaccatcatt ccggaagaat
    26341 tccgccacaa acccattcgt ctccagacgc tggtcgatat tctggtggcc gcgtttatca
    26401 agcggctcag tgccggcagg tcggacgggg tggcggttct cgccgaaggc ctggtcgaaa
    26461 ttctcgacca acaggatttg aacggactcg aagatgtgga acgcgaccag cacggacatg
    26521 tgcggctggc ggagattaat ttcggcttcg tgctcaagcg ggcggtggag aaggaactgc
    26581 gtacattcgg gatcaagact accatcgtgg ataaaaatat cgggtatgaa ctgcgctgcg
    26641 cggatcctat ttcattcgat atggaatata cgcgggacct gggctactgc gccgcgcaat
    26701 ttctgttgga aggcggcaat gcggccatgg tgtcgattca aaacgggcgg tttattccta
    26761 ttccgtttgg cgacattctc gacccggcca ccggccgtac gcgcgtgcgt atggtggacg
    26821 tcgagtcgga gtcttacgtc atcgcacgtc ggtacatgat ccgcctttcg tccgacgatc
    26881 tgcaccaacc ggatgacctg gttcgattgg cgaccaccgc cggactcgcc tcggatgatt
    26941 ttcgtaaacg gttcgagtac ttgatcggta aggatgtgta aggagtgttg cagaacatga
    27001 tgtctgcccc cacgcccgac acggaaacga cgatcgcctt tgagaaccag cgtgcggtgc
    27061 tgtcaccgtg gggcgcctcc ttgcggcggt actttatcgt cggcagttct ggaagcgaaa
    27121 cggacatcgt atggggctat tcaggcggcg ggcagaagaa gggcgggcag ggcgatgtgc
    27181 tgattccatt tccaggtcgc gtggcgggcg gacgatactc gtttgacggt caactacttc
    27241 aacttgaacg gaatgataag gaaggcccga atgccattca cggatttgtg cgaagcctcc
    27301 catggacgac gcatcaagcc gtcgctcacg ccgtgaccta tgaagtaagt gtggatgccg
    27361 ccgactacac ctcgcgaggg tacccgtttt cgctcgtcgt gcgtctcacc tacagtctgg
    27421 gcattcaggg attgtcctgc acattttctg tacggaatgt gggaggtgcg gcagctcccg
    27481 tcggagtagg attccatccc tacttcacgg tcggcaccgg gctgatcgat gaggctgagg
    27541 ccaaaattcc tgccgcaggc tttctcgaat tcaacgagca gctcgcgccc accggcaagg
    27601 tgatcgccgc aacaggaact gaatgggact accgcacgtt tcagcgcatt ggtccgcgcc
    27661 gcttcaacca ttgttatctt gaattagagc gcgatgcggc ggggatggcc acggcatcac
    27721 tgcgcaatca caaaagtgga cgcacgattg atgtggtgat ggatcgtgct ttctcggcgg
    27781 tcgtggtcta cacgggggat gccatcgctg atgcgcctcg ccgcgccctg gccatcgaac
    27841 cgatgacttg cgcgaccgac gcctttaatc accccgactg gggactcaag cgactgcttc
    27901 ccggtgaggt cttcaccggt tcctactccg tcagggcacg cagccgttga tctgttccag
    27961 ccggtgatga cggtgcggct ttctggtgca gcgcaccagt tccaatatca cggctgtctg
    28021 ttacgaaggc cgcgtgggtt cgatgatgcg gaaccgcacc tcatcaacct gggtataggc
    28081 cgacgtttct tcgcctgcaa acaggccgca ccggtagagg ccggctgtcc agcctgtggc
    28141 cggacgaggc agcacaaaat aaccggattg atcgttcgtg gacatgatga gccggtcttg
    28201 cgcgacggct gtgtgtgcgc cggccagttc ggcggtctct ttgaagcagc gtgcggccag
    28261 gggcacctca tcatatgagg cggtgaccag gctgaagacc agatagatat cctgctgact
    28321 gcccgggaag ctgatacccg ggtccaacgg aatgaagtca tgcgcgccgg tcggtgaatc
    28381 atcgcgcatt actttttttg ctggaatgac gaaacgaaac caggcgaagt cggcgtcccg
    28441 gccgacgagg gacgccgcag gatccagcgt gttttgcggc ttcaaagctt cgatggcctt
    28501 cgcggcgagt tccgcctccg gaggaatcgt cggagccgcc gccttggctt gagccgccgt
    28561 ggctgcagcg tcttcgtcga cctgctgggc aaactccggc acgtgcaggc ggaccttctc
    28621 caaccactgt acgaggttgg ccttattcaa gacacgggtt ccggagggga gctgcacgtc
    28681 cttcgtggat ttttcaaact ccacggtcgc atcgtacagc gtcttgaact gcatgtaggc
    28741 gtcttcccag cgtcccagct ccaccatgca ttggcccata cgaaacgccg tggtcgtatg
    28801 ttcttcatca gtcggatcga attcagaccc agcttgtgac actgcgagcg cgtccttgca
    28861 acggcccaac cccatgagga tgagactgag atgccgcccg gccagcggat ccatcgggtt
    28921 gaggcgaaga agttctcgat agaccggctc cgcttcacga aaccgtcccg cctcaaagag
    28981 ccggtacccc tccgcgcgga tcatcgacca gtcgcgtaat tgtttcgggc tgacatccgg
    29041 tgtcatgacc ggtgcaaatc ggcgcccccg ctccgtcgag ccgaagaaga cggtgaggag
    29101 atttctggca gagaggtgaa gcggcgagcc gggcgggact cgatccagga cgaactgcac
    29161 gtcacgttcg gcgccttcgt aatcgagaac atcaaaccgg gcccgcgcca gcgccaaccg
    29221 ccagttgagc atgtccggcc cgagttccac ggctttctgg taggcctcga aggattcttc
    29281 cagccggtcc agtttccgga gttcgcgagc caggcgcaga tggagcaggg ggttgtgcgg
    29341 atcgattacg gcgatgcgtt gaagctcggc gaccgcttcc tcgacctgtc ctgatctcac
    29401 cagcacactc cacttggccc accggacgtc gagggcggca gggatgcttg ccaggacagt
    29461 ctcgtacgat tcaagcgcgg cttcggtttg ccgtgaggtg gccagcatgt ctccgcgaag
    29521 ttgatgcaac gcgagcgctc gcggatggtc ttgaatgccc tgctcgacga tgccgagcgc
    29581 aagattgagc gtcccctgtt cccaggcggc cttggcatgt tgtgcgatct ggccttccga
    29641 gcgaagcggg aggtcattgg cgaacgcgcc cgaaccggtt cccgcaatgg gcgtgagaag
    29701 caacatgacc gataggctga gcagccgccc atggtggcga acctgccggt ctttgggaac
    29761 agccatacga aatctatgtg tgatcatggt ggatgtctct agtgttacgg acgagccgtc
    29821 tcctgtcttt cgatcattag aggcagaacg agtctgaagg attcgccttc gcctgatgga
    29881 tcgagacata agactctatc ttactgtatg aacgtctagc cctgcgagcg caccggggga
    29941 ccacgccatt atgtgaaagt gatgccgtgt gggcgccgag agaaagtggc ccgatcagaa
    30001 cgacgaagag cggaacccgc tcatcgaacg ttgacgaagc ggccttgcgc ggtggctcgc
    30061 agctgattga tctcctcact ccgcgtcacc gaaagcggca cggtatggga gagttcctgg
    30121 atgagaagat cggtcgtcaa cggctgtttg tgatacagcg cacgatatag gccagcgacc
    30181 acggcctgtt cgatttccga tccgctgaat cctttgctgg cgtccacgag cttcgggaga
    30241 tcgaattgtg cgcggtcttg tttgcgaagg ttcagatgga tcgaccagat ggcggtacgt
    30301 tcgtccttgt ctggaagatc cacaaagaat atctcgtcaa agcggccttt gcgcagcaac
    30361 tcgggaggca gagccgagag attattggct gtcgccacga caaagacgtc ctgtttcttt
    30421 tcctgcagcc atgtgagaaa ggcgccgaac agccggcggc tcaggccccc atccgcatct
    30481 ccgctgccgc ctcccgccga catcgccttt tcgatttcat cgatccagag cacgatcgga
    30541 gacagggatt cagccatctc gatcgctttg cgaaagttct tttccgattc gccgacgaat
    30601 ttgtcgaata accggcctgc atccaatttg agcagcggca agtgccattc gcgcgcaatg
    30661 gctttggccg cgagcgattt gccgcatccc ggcacaccga ccagcatgat gccgcgtggc
    30721 ggggtcaggt tgagcgcctt ggcttcggcc gtgaagccca tcttcgcccg ctccagccag
    30781 gacttcagtt tcacaaagcc gcccagttcg aaccggttgt cttccaacgg ataatattcc
    30841 agcaggccgc cgtctcttat ggcttggact ttgcgcgtga gaatcgtctg cacatcgtcg
    30901 gcggacagca ctccatcttc gatcacgcat tgggtgatga cctgccgtgc ctgatgcaag
    30961 gtcaggcctt gtaaggcgcg aaggatggcc tcgcaatcct ggtccgatgg gccggccatc
    31021 gtgggcttgg gggtggccat ggagttgagc agactctgca cgacggtggt cgaggttggt
    31081 cgacggggtg acgttcgcgt cctcaacgat tggaggacgc tctgaagcat cgcgtgtaac
    31141 tcatctcgat ccgggagctg cagatcgaac cgcaccgcaa tctgatcgag gtcgagcggc
    31201 agggtgatgg gatgtcccgt catgacgcag gttgcccgtg aacggctgag gtgatgactc
    31261 acctctcgca gttggcgcgc gacagcggca tcctgcaagt gcggggcaag atccttcaac
    31321 cagaaaatgc cttcgacggt caatccattg agatgttgaa gcgccgccag tggggtggcg
    31381 gtcagcttgc tgaggctcgg cccttcctcg ggcctggtga ggcctctcgt aatcgaccat
    31441 tcgaacaggg gcatgcgttc ctgggcggcg acggattgca gcaacgtcat cacccggtct
    31501 tcttccacgg tctcgatcac aatgagtgga tgagaggagc gaatcagggt gcggagatcg
    31561 tgcacggtgg ttgagagcgg cataggaatg aaatgctatc acgggcccca cgcctcacca
    31621 agaaaagggg cgattgtggt gggagtggga aacgatagca gggatagaaa tggatggtac
    31681 gtagcgggga agtcccagtg atcgtgcatt catgttgtgc ggatcaaatt gacaggtgat
    31741 tccagtgagt caacaatcac caggtaaagt aaaactctgg aagagaattc atatgacacc
    31801 tgacttggaa aattcctgca aagacgaaga agcgcgggca actccggtcg caacattctg
    31861 gcgacgtacc ggagccgctg tggcggacgt gttactcctt ggagctatcg gggcttgtct
    31921 tggtttcctg tggttcgatc aattagccgc gcttggacga acgggtcgat tcattggtgc
    31981 tgcaattgcg ttggtgtatt tcgccacatt gaatagtcaa attggccagg ggcagactct
    32041 tggtaaacgc ctcgcgaaaa tccgggtaac agatgcgaag ggcgcactga ttagcctgcc
    32101 ccggtcggct ttccgtgcaa caatcttttt cgcaccattg gcacttaatg gaacagccac
    32161 tcctccagga ccatatgaac agcttgtggg cattgtcctc agtattctga tctttggtgt
    32221 aggtgtggca caggcatatc tctactgttg caatcgacgg acaggtcaag cccttcacga
    32281 tatcgtggtt ggaacatttg tgagggaggc ggagagcgct tcagagattc aacagaagat
    32341 gtggaagcct catctcgcga ttgccggtgg actcagcact ctagtgctcg gggtcttgct
    32401 gctgccttac acctctgatc cgcctgattt tgtatcgcag ctggcagagg cgcgacgcct
    32461 ggctcaatcg gtggtaccgg atgcgcatgt gtcgatcagt cggcagactt ccatgatgac
    32521 gactcctggg agcgggcaga cccccgcttc ccgggtgcag cttagtgtgg aggtgcgtgt
    32581 aaaaccggaa aatttcgaaa gtgttgcaga caacatcgct atggcgatgt ttcggtcttc
    32641 gtccggagtt ggtgatgttg ggcaagtggt gatagtgatt tcgtatggct acgacatcat
    32701 catctcgcat gcacgaacaa caaaggtgtt cgcgcatact cctcaagaat ggcttaagcg
    32761 tctcaagctg gatgagttac ataatgtcgt gccgcctgca taaccatcaa tggctggaag
    32821 tgataacaac gagtaggatt gttaggtcgg ctacaggtac gttctctaaa tcacccgcac
    32881 agtgactcgc cccttttttc agacttaggc gtcgttctcc gtgaggcagc gaccgagacc
    32941 ctgttgttcg gcaaggtaat aggcgtagtg gatggctgcc aaattcgcga ctctctcctc
    33001 ggcctcgtcg cgactgtctg cataaatgat ctgcaggccg aatcgtgcgg taatggaatc
    33061 gagaaagtcg atcaagccat tgcgatgata ctgttcgagc cggcccccac cgcgatgaga
    33121 atgcagggcg ccttcgatga cgagaaaacg atgggggatc gcgagcagtg gctcgagttc
    33181 gcggaggaaa cgcgggcgat tatcggacgg atttgagaca atcgtattaa actcctccgc
    33241 acgtcggcgt tcgacacaaa agatgccggg cgcttcggcg atggcgtaat ctccagccgg
    33301 cagggcctgc gtgcgcgttc ccgcgatccg aatgagccct ttgaatgcgt agggttgggt
    33361 ttcgtgcacg tcgaccagaa gatgaatggc agggaccatg attttgggat gactgaaccg
    33421 ggtgacgttc agcatcgttt gcggtccgcg tagctcctgt cgcgggcgtg tcaccggttg
    33481 gggacgtggg ggcgaagaag gtcgcggggg aggcggcggt gctgagggct gttgccttcc
    33541 gctatcgtag cgggcgcgcg ctgtgggatc gctgagtgtt tgataggcct gattgatcaa
    33601 ctgcgtgcga gcttcggcct tcttgtgcag ggtggatgaa tgtaggaagc ggtcgggatg
    33661 ccagatctgc atctgttcat gccaggcgcg tttaatatca gcctccgtcg cgtcagcaga
    33721 caattccagg atggcgtaat agttggtgtt gtgttggtgc tgcatgggta tccctgcgtg
    33781 ttcgaagtgt aacggagtgc gacaggaaac ggctagattg aaataaggaa ggccatgtga
    33841 ttccggccgt gccattgtgc gagccttcga ggcggtgtat cgtaggccgg tagaggcggg
    33901 tgttctcatc tcatcccagc ggaggcgatg cgcatgatct tggtgacggg gggcgccggg
    33961 tatatcggtt cacatacctg tgtggaactg ctcaatgccg gctcggcagt gaccgtgttc
    34021 gacaactttt ccaatagcca tcctgaagcc ctggcgcgtg tgcaacgcat cacgggaaaa
    34081 tctcttcgtc tgatacgggg cgacattcgg gatcgggcgg ctctcgttgc ggcgcttcgg
    34141 gagagcggcg ccacctcggt catccacttt gccgggttga aagcggtggg tgaatcggtc
    34201 gaaaagccgc tgtcctatta cgacaacaac gtggtgggat cgttgcgtct tctcgaggcg
    34261 atgggggagt gcggcgtcac gacactcgtc ttcagttcgt cggccaccgt ctatggagac
    34321 ccgcaacgct tgccgttgac cgaagatcat ccgctgtccg cgaccaatcc ctatggccgt
    34381 acgaaattga tggtggaaga gatcctgcgc gatctgcagc gtagccatca ctcctggcgg
    34441 atcggcatcc tgcggtattt caacccggtc ggggcgcatg ccagcggcct gattggagag
    34501 gatccgcaag gcatgccgaa taaccttctc ccgtttgtgg cgcaggtggc ggtcggccgg
    34561 cgcgaccatc tgaatgtgtg gggcaacgat tattcgaccc cggacggaac gggcgtgcgc
    34621 gattacatcc atgtggtgga tttggcgctc gggcatctca aagcgctgaa ggcgctggat
    34681 ggatgggacc gcccgaagga atgtctgacg gtcaatctcg gcacggggaa cgggtacagc
    34741 gtgctggaga ttgtccgggc gtttgagaaa gccagcggga aggcggttcc ctacaagatt
    34801 gcgcagcgcc gtcccggcga tgtcgcctcc tgttacgccg atccagggta tgccctcaaa
    34861 gcgttagggt ggcaggccgc ccgtggactg gatgatatgt gtaccgatgc ctggtgctgg
    34921 caatacaaca atccgaacgg atacacaggt tgagctgatt gcggatgatg aaccggctca
    34981 caagagatcg cgcgcaaaca gatctgctca cccaccgtca tcgatctgtg gtctgggttc
    35041 ttggcgccgg tgcgctcctg agcgcgttcc tgtttgtacc ggccgcgcag gcgaatcacc
    35101 gcgatcatca accccatgca gacgcggaag tgcccggtgt cctcggccag ggtcgagaaa
    35161 tcccgggtgc accttttaaa gtctttctga gcacaggtgc agtagccgat gtcagcggac
    35221 tggatgatgc cgatctcgat gcggctgtcc agacagtaat cgacaccttc atcattatgc
    35281 tgcagcatcg tgccgactat ccgcgttttg aggagagcct caagaaacag gcgctcaagc
    35341 aggtcgtaat cgaaccggtc gtggtcaatg acgaggggaa agcctttccg tttttggtcg
    35401 cccgcacgaa agaaccgggc cgtgtcacac tgctgatcag cgccacctcc ctgaaggaca
    35461 gagggtatct caatcatccg gattccctgc tgcctgtgct gtccagagag tttcagtggg
    35521 tggtgagcaa ggccgatacc gcaccaaagg cgaaggcggt ggccgtggaa cgaaacctgg
    35581 agaaggcccc catcctcacg gacgaagaga ttgcgggtct ctctgccgaa gaacgggttc
    35641 ggttgctcca acaactcttc gacacctatc tgcgaacggt agacgatcag aggagcctcg
    35701 acggacaacc ctggtacgaa gtcggccatt ccgcgccgct accgccgact catgcggatt
    35761 caaccacgaa gttgtatgac atccgcattc gtgaagcgct gcagaagatc gtgcgtgacc
    35821 cgttttttct ggagcgaacc ccgaaggcgg tccgcaatct tctcaatggc aaagtctgga
    35881 atgtcgccta tgtgaaaatc gatcaacgcg actggtcgac ccgcacgagg gtgttgtccg
    35941 aggaaaagtc tgtggtggtg ggaaaacggg agcggcgcat acaaccggcg gcgattctgg
    36001 tcaatgtcca ccgcctcgct gctgcagatg acccctttta tcgggagacg caggggctgc
    36061 cgatgggagc cctatcagcg gatcaactgg cgcatgtcat tgctctggag atccatcaca
    36121 acattcagga aaaatccctg agcggtcaca cggctcagga tgccctcacg gcgccccggt
    36181 aagcggcgtc tcacttacgg cgcaatggtt ctcgcctcgt tcttctgctc gtatcgcacg
    36241 gtgtccacgg aaaacagcgg cgcggcgctg ttcgattggt gagccgtcat ccgtggcgat
    36301 gggccggatt gcaagcgcag ggtggtcagc agctgccgaa agcgggaggc gtcggcatcg
    36361 gtataccgga cctttgagac atggatgttg gcatagacgt tcccttcggc catgcaggcg
    36421 tacaaactct tttgatcgag acgaacaccc tgaaagcggg ggagcgtgta ttcgagcgtc
    36481 ggcatctcgt gtgcagtcga aagagtgacg tcttttcctc ggctcaccgc tgatcctttc
    36541 tgcacgtgtt gcaaatgctc gatgcatccg gcggtcgaag cctttccggc cacctgctcc
    36601 aacgcgatgg cgaccttgat gcctgtgcct ggatgggagg ccatgaggta gcggcgcccg
    36661 ttgggtttga gttgatcgcg ttcaatggtg aagccggcga gatcgatgga gagtcccaca
    36721 tgactgttcg attgggagac gattgtacga gcactgcttt ccagtgctgc cgccggtagc
    36781 gctccggtca acagcgacac cagggtcaga cccacacacg caaaagacat cgatcggacg
    36841 gtgccggctt gtgatctatc cataggtgct cctctcctca agcctgtctg tgcatgccgt
    36901 cagtgtatgg tgaaccggaa cggatcccaa gcgatttgct cattccggcg agcgtacatc
    36961 aggatgacag gtagggaatc ctcttcactt ttcctcgccg gtgtagagcg gtgcacagta
    37021 ccgttccact cgtgttgctc tcgcctgtgc tgccaacgcg atctccgata gcggccggtc
    37081 cccataccct gaggcggtca aatcggccat tcctgtagtt ggattccatt gcgttgtcct
    37141 acgttgatct acatcgcgga gaaaatctct gtgccgacgg cttttctctg tcctgaattc
    37201 ttgacgtgat gcatggctcg gtgtattttg cccgcctgat tttttgtcta cgtattcctg
    37261 cgttgctgtg atgagcgaga acgactgcct tcgaaggcga ggaaagggcg ccggtccaag
    37321 gccggccgtc ctggaaagca actgaccgaa ccattgtgat ggagagacgc acatgggccg
    37381 ttccgggatg ttgttgtgtg cgatggtcgt gatgtgggcc ggtttatggg aagtcggtgc
    37441 ggcggcagag ccgtcgtcgg ttcctgcggc agaagccctg cgaatcatgc agttcgtcct
    37501 gacagggact gtgtcgacgg aacgcgcgcg cggatttgtg aatgaagccc aggctgcagg
    37561 gtttaccgcg gtgcaggtgc tgctgaccga cggtgtgcag ttcgaccatg ccccctggat
    37621 acccgtgaaa accgcctgga cgaaagcgga gttcctgtcc tgggccgatt atgcccgtgc
    37681 ccatggcttg gaagtcattc cagaggtcaa gctgctcacg caccaggagc aattctttca
    37741 gcgccaatat ccgggcttga tgttcaatac cgtgtcctac gatccgcgga aagatgcgac
    37801 gtatgcggtg gtatttccgt ttctcgacga actgatcgag gcggtgcatc cgcgggcgat
    37861 ccatatcggc cacgacgaag ctttcggatg gaccgtcggt caggtgtcga aatggttgaa
    37921 gctgggtgaa gtgatgatcc cggcggatct ctttctgcgg gatgtggtgc gggtgcatga
    37981 ttatctcggc aagaaggggg tagaaacctg gatgtgggcg gacatggtgt tgagcccggc
    38041 ggaatttccc ggcgcgcaga ccaggcatct ccatggcatc gcggcgggat acggcaaagc
    38101 gctacgccag cagcttccac gggatattgt gatgtgtgat tggcactatg gcgatgaaca
    38161 gggaagtttt ccgtcgattg acatcatgca aggggaaggg tttcgagtca ttggtgcgac
    38221 ctggaaacag gagtcggcga cgcgcaagtt cagccgctat gccttatccc ggcacgccta
    38281 tggcctgatg gccacaaccg ggtttcatat tcagcagaac gataccaaaa tgatccactg
    38341 gatcatccag gcgtccgggg ctttgtttcg caatcccgag gcagtggtgc cgcctatgcc
    38401 cgcgtcggct ggttcatcgg aaggaagggg ataatccgcc gactgcacaa tcggcaagtt
    38461 gactccatct atcccgggca tgggccatat ggttcatgcc cgggatctgt gctccattca
    38521 tcggccgtcc gtcagctgcc tccaaggtct tctccgtatc cctctattga tcattgagaa
    38581 tgtgaacgtg cagttgaggc tcgtccattt ctgccccgag tgcaggggag tggaccgcac
    38641 cagccttgaa cacgtagaat gcgcctcttc ggttgacgct gccccttcag cagtagagat
    38701 atgggaacgg cgggccgaat caacagggcc atgaccagga agggatcgtc atgagtgtga
    38761 aatgtggaat cgtgggactg ccgaatgtgg gcaagtccac cctctttaat gcgctgacca
    38821 aatcggggat cgccgcggag aattatccgt tctgcacgat cgagccgaat atcgggatcg
    38881 ttgaggtgcc ggatgcgcgg atgcaggcgc tggccgatat cgtcaagccg cagcggatgc
    38941 agtacgccac gaccgagttc gtggatatcg cgggattggt ggcaggggct tccaagggcg
    39001 aagggttggg aaaccagttc ctcgccaata ttcgtgagac cgacgggatt gtgaacgtcg
    39061 tgcgctgctt tgaggacgat aacgtggtcc atgtggcggg aaaagtcgat ccgatttctg
    39121 acatcgccac catcgtcacc gagctcgcgc tggccgacct cacaaccgtg gaaaaggcgc
    39181 aagagcggaa tgtgaaactc attcgctcgg gagacaaaga cgccgccaag ttgggcgacc
    39241 tgcttgtgca ggtcgcggct tgtctgaacg agggcaaacc ggcgcggaca atgaagttgg
    39301 atccggcgca acgggcactg ctgaaaccgc tgtgcctgct gacgatgaaa ccggtgatgt
    39361 atgtggccaa cgtgtcggaa aaagggtttg ccaacaatcc gctcctgacg cgtgtggaag
    39421 actacgcggc ccaggaaggt gcgccggtcg tggcgatttg cgcagccttg gaatcggaga
    39481 tcgccgtgct ctccgacgag gaaaaacagg aattcctggc cgatatcggg atgaaggagc
    39541 cgggactgaa tcgccttatt cgcgcggcct atcagctcct cggcctgcaa acgtacttta
    39601 cggccggggt gaaagaagtg cgcgcctgga cgattcatat cggcgacacc gcgccgcagg
    39661 ccgcaggcgt cattcacggt gacttcgaaa agggattcat ccgggccgaa gtcatcggct
    39721 acgatgatta cattgcgtgc aagggggagg cgggcgcgaa ggaaaagggc aagatgcggc
    39781 tggaagggaa ggaatacgtg gtgcgcgacg gcgacgtgat gcacttccgt ttcaacgtct
    39841 aggttagtgc catgaccatg ttatgaggca gtgaattcct gcctgattgg agatcgatga
    39901 tgaacgaaca ggatacgcag cgcgcggtag cgattctcaa ccggatcatg gagctggaac
    39961 tggcgggtgt cgtgcggtac acgcactatg cgctcatgat ctacgggtac aatcgtattc
    40021 cgatcgtgtc gtggttgaag ggcaacgcgg atgagagctt ggcccatgct cacaaggccg
    40081 gcgaactggt gaccctgctg ggtggccatc cgtcgctgaa gatcgggacc ttgctcgaaa
    40141 ccgaaaaaca cgatatcggc gatattctgc gggagagcct ggagcatgag aaggccgccg
    40201 tcgcgtctta ttacgaacta ctgaagctgg cggagggtaa atccgtcttg ttggaggagt
    40261 ttgcccggga aatgatcgtg ggcgaagagc tccatctcga cgaagtgaat aagatgctgc
    40321 gcaaatcggg tgatgtacag gcctttcgtt cctgatcatt aattggctgg aggggtaagt
    40381 tgtccgggca accggctggc tctcccctcg gctcatagcc gtcgatctcc gccgatccat
    40441 ctccctgtca tcatgtttga tgtcattctg tatcagccgg aaattcctcc gaacaccggc
    40501 aatatcatcc ggctgtgcgc caataccggt gcccggctgc atttggtgaa accgttggga
    40561 ttcacgctgg aggacaagca actcctccgg gcagggctcg actaccacga attcgccacg
    40621 cttgccgttt atgagacctg ggagacctgt ctcgaacggt tcactggtca aagactgttc
    40681 gccgtttcga ccaaaggaag gcagcggtac gatctggttc ggtacgcgga gggtgacgtg
    40741 ttcgtgttcg ggccggaaag ccgcggtctg ccagcggaga ttctcgcaag tgtctccgat
    40801 caacaacgca ttcgtgtgcc gatggtacct ggaagccgca gcctcaacct gtctaattca
    40861 gtcgcggtgg tgctctacga agcctggcgg caggcggagt tcggacagtg cctgtgatgg
    40921 tctcgaatct gctccttgcc gtccgttagg cagatagatc gtttcaggac aacgcttacc
    40981 gggagtcaaa tctccagtac caatccgatc tcgaccacct gctccgacgg cagatggaag
    41041 taagaactgg cgcgctgggt attgcgcgac agcaccacaa acagtttttc ccgccaga
//
LOCUS       DAOQEW010000010        40966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_184_length_40966_cov_9.681455, whole genome shotgun sequence.
ACCESSION   DAOQEW010000010 DAOQEW010000000
VERSION     DAOQEW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 40966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40966
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_40966_cov_9.681455"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLS35_02190"
     CDS             complement(<1..398)
                     /locus_tag="PLS35_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17488.1"
                     /translation="MPTTWGNIKEEQMDAFRDVTKSYIGDSELLRMLRRVLRMIGRGQ
                     AYTFQQKETVLTLTGAQQYDLETLLPRWCRIISITNPVSQDAAVPLEMDYMDIKNFAL
                     TVDRFAYSIIGNRYLRVFSPTQSPLSGSLR"
     gene            complement(539..1021)
                     /locus_tag="PLS35_02195"
     CDS             complement(539..1021)
                     /locus_tag="PLS35_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17489.1"
                     /translation="MPSPIVYYRQNDPLWANVTIGKTKLPLWRWGCTITALCTLLSSR
                     GYKITPGEMAKLPGLYNDQGEMIWANLPKATKGMVKACEGRIYGRNDAKIRTSILGGP
                     TLVAMLQVEIKKDKHWLAAIGVLPGGEDYWVSDPLDGKRKQLSKAYPGITGSAHLILS
                     "
     gene            complement(1126..1398)
                     /locus_tag="PLS35_02200"
     CDS             complement(1126..1398)
                     /locus_tag="PLS35_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17490.1"
                     /translation="MSEELALGQREITVETQFDSLSAEEVTLLGRAAFSLVLDIVKGS
                     GTQTLNAAIGELAGNADAGLDASAKPEAAATDKASTGETTEEAKTE"
     gene            complement(1571..1798)
                     /locus_tag="PLS35_02205"
     CDS             complement(1571..1798)
                     /locus_tag="PLS35_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17491.1"
                     /translation="MATERRIMHFVLNDGCTAAVLITPIEYARAWKMAQDTTLEILVQ
                     VVNPDGVLKKLDIREFEKKRLNGFAQQRKGL"
     gene            complement(1777..2043)
                     /locus_tag="PLS35_02210"
     CDS             complement(1777..2043)
                     /locus_tag="PLS35_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17492.1"
                     /translation="MHIWFKRSKENPDGFRGENTMTLKSFKHIVEEVQTGNISWFRIN
                     VKIGRYPFSFHIEKVDVERTLREAEKELNFRPKGEMASWPPSAA"
     gene            complement(2123..2419)
                     /locus_tag="PLS35_02215"
     CDS             complement(2123..2419)
                     /locus_tag="PLS35_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17493.1"
                     /translation="MSESSPFEITRAVLPIGAAVAAVVVIISLIGGGIVYVTGIASTS
                     TQNSKEIEVLTSRINTIDVSQQALSTKQSVQDVKLDTILDRIQEIKTALEKTQP"
     gene            complement(2423..2713)
                     /locus_tag="PLS35_02220"
     CDS             complement(2423..2713)
                     /locus_tag="PLS35_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17494.1"
                     /translation="MQIAWRLDKETIKKILIGIGISVGGAVLAGFVVLSKDIGQFILQ
                     GTAIDWRSAAFAVWGALSTSIVNTAWQFMKGVSPEVLKNANVNLDNFQGPQA"
     gene            complement(2719..3069)
                     /locus_tag="PLS35_02225"
     CDS             complement(2719..3069)
                     /locus_tag="PLS35_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17495.1"
                     /translation="MDKEDVQKAGAAIGIASIVAIGGLVSFVFDGKDPKTEGTLQVCV
                     DGKNCVAYSETDYATLKAEAAALLDKPGGMTWQDLQAVTAIADAAIKERGGLSIKLDE
                     QNTLKDELKDLLTQ"
     gene            complement(3080..3868)
                     /locus_tag="PLS35_02230"
     CDS             complement(3080..3868)
                     /locus_tag="PLS35_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17496.1"
                     /translation="MKFLENSAKKFGISAVVSLLVISSAVFPAYALTDKDLTITQWDE
                     GNFLFQPYYFQPTMDGRSQILGMRGDTQHPFLFTMGHGLSNNTSTGEVVVGGLDQADI
                     SGLTADLAAKADAASLATVATTGDYNDLLNKPSTSWTFATSSRSLTTGTGATGFQVSA
                     TQNVTVSYAVNIATVATLGAPAAGYITMEIAPTNSSNAADWYEVGTRCRADNTVGLLA
                     VQSTQGNGCSLSAPIPAGYYVKLRSVTVSGTPTFTFVSGWETKQ"
     gene            complement(3922..5295)
                     /locus_tag="PLS35_02235"
     CDS             complement(3922..5295)
                     /locus_tag="PLS35_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17497.1"
                     /translation="MKKILYSLLTLFSVAIMVIAGGVQYANSQTLSPGGGTGVLQPGL
                     FYLKNGAILTTNPAYTIGSATNPIAAGYFTFLSAATASISGIGTGDMLPSANNTFSLG
                     SATRTWANLYLGTNAYVSGTINTNNLIWTNATGTNTTSTNLFATRLLFTNATGSGALQ
                     AQSVSSTSATTSWMTITSKVGNSGKINITNSNLNCAASQTACIRTDTGAMAISAGTGG
                     AQLYFNIDNVGGTSSFYGNILPDNTTRNLGSLTLPWNLFAGSVSSTSVTSTKMYIGNS
                     PGGCASLTGGVVGIASQGTGFYINASHQIQFCTNGNTGPFLDPGEGWFATNVVPLSTG
                     TGNVGSSIRVYGQGWFNHTTGTDAYYSQGIAIAGTSTQATSTNYTINATNFSFKFPAA
                     ATSVIINDSFVRGTTSTHVQVTIADLDATMFYAQVTTTAVGQIRVRAPAAPTADTKVN
                     VLIINER"
     gene            complement(5299..5844)
                     /locus_tag="PLS35_02240"
     CDS             complement(5299..5844)
                     /locus_tag="PLS35_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17498.1"
                     /translation="MYDPQVSGYNPQYWKTVVGASDPSVTADGIVLTNTTIETYISHR
                     YAGLLLNLKLPVDPQAAQNSFWGFGSVSLGNRGKTGFIIENQVFKAVAYDNVGTLIKS
                     TTITWDASWTNNTTTVFEVVWRETGVRWLINGVKVAEVSTLLDGFSAMPTNLGLPIML
                     AADANTVNMFLMDIRDLQART"
     gene            complement(5893..6306)
                     /locus_tag="PLS35_02245"
     CDS             complement(5893..6306)
                     /locus_tag="PLS35_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17499.1"
                     /translation="MKFQSSAKNLRLIKRADWIEPVQGAPAIRHAGEYITFTNGVYET
                     EDEKEIEWLKAHKLFSVIEKAPQSFWVYREPIDISKVLKDKDEEIARLNAENESLKAE
                     NDGLQVRLNIRKSAKEKAPAEPPAPPVGEPQVATT"
     gene            complement(6392..7186)
                     /locus_tag="PLS35_02250"
     CDS             complement(6392..7186)
                     /locus_tag="PLS35_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17500.1"
                     /translation="MNADTLAVANGLAQKRSCVATGDDSSIRQFLGMRILYKGSSASA
                     LIKTSGNNAILSLIGAAGSEAADAAFTYPSGGTPGTISLNAGGFSTMGEVVDFINNLP
                     GYSARLIGLRRSDPCSTTGALVADSTGQQAKSTNGAPLIVDTSVAGHITAEISCLDGL
                     MNKSAADTDMKRIAVNALHLVAMTLQHTAGVFEVISVNDQEKTDEVIYSRLIAVQFPV
                     NASSVDFTNSSGIGINARPGRRLLVRARGAVAVTNMNVVSTQKVLN"
     gene            complement(7212..8381)
                     /locus_tag="PLS35_02255"
     CDS             complement(7212..8381)
                     /locus_tag="PLS35_02255"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HNI17501.1"
                     /translation="MDFPFITFLGMNGRVYGGNPYGVEGPIGEMYGGEVNVSGQALSK
                     RKVLNAEYQIFEDEVLEDTTTVNLVAGYSASATSIVVTDASIFSKNDQVLVPRTSEVF
                     VVTASDLTTNTLTVVRGVGSTAAALVNADYLVRLASAYAVNALSGTAKMTVPTRNYNY
                     TQIFRTPVQIGRTDKDSKVNYSPTSDLDRLKRQAGIEHLRSQERAFLFGGREERAAVD
                     GSGTRQRFTGGVFSLLTSNVMDLSGSGGVMTQGSVDAFAELVFARGSTEKIAFCSPRV
                     LSRINGLYDNLIRYEPETKMFGSNVMELVTSHGVLKLVRHPMFGKGGIAQKHAGTMLV
                     IDPASVKYAHLDQAENMYHDNIQENDRDGYKGEWLGECGLHLANETAHGVMLNVL"
     gene            complement(8525..9316)
                     /locus_tag="PLS35_02260"
     CDS             complement(8525..9316)
                     /locus_tag="PLS35_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17502.1"
                     /translation="MADSQDTANLPGAEGTSGETTPTEPAKDGEAGSEKVVDPVKPDA
                     GKQDDNEYFHVEGDVKFKTKEEFLEHAKKRIGDASRVIGENFNLRKEIEELKGGKQPQ
                     KTDSKDGGNTPAEEFSDEEKAVLQKLGKVFLSREDLEKLLSERLSDISPVVNRIRSQE
                     QQSANDLVAEFMQANPDALAIANDLASAVEKLGSSGLQVDLYDAYRLLTKKEPVRPSQ
                     EPPKKDDTEEEKTKIKQAQAGGAPSGAAGKPAKKGDDFFAGLFRG"
     gene            complement(9395..9694)
                     /locus_tag="PLS35_02265"
     CDS             complement(9395..9694)
                     /locus_tag="PLS35_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17503.1"
                     /translation="MTPEQKAQEELNERIKIGRSFVILMNSPEWSVTVDQFLVSLQEE
                     MEKLTLTSIPPDKARELCWMAHGRQEFINRFKEQMESWISDASLTLDQPKEGSAS"
     gene            complement(9710..11797)
                     /locus_tag="PLS35_02270"
     CDS             complement(9710..11797)
                     /locus_tag="PLS35_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17504.1"
                     /translation="MDQLPIFQNIQRGPAEKQVKMTKEEQEILKRKDEQFGASKAAKA
                     PIKQMMEASHLSFNQILSDWAFAQRDIAKYGPAIYVPVTFQTVASIQAQLNGRPPRYR
                     LSPQNTPQDREKANFVSKISQGEMKRAQAMVAISNSVQESLIFGTSFLRSVFRYDRRK
                     MKFVKGVDRSEEGKSPVYEEREKVFYKGWVPQNDHPLCVYLPAVHEDDPSRWPFYIYR
                     DLVDTRNVKAYYEAHPELAYKNVYKGLCAGGDLTDDLECLFKTDPMYDYYSMQRYPGS
                     TRDALGGRKAQPYAQLKSENLSERLTIYDQENDEWTVVVAGRVVEHHPNPLEDTKENP
                     ITVMRDYKVSRVPWGIGEPHLLRYLQWEANALHTLALDGTKFTTAGVFGVNSAYLKNP
                     ADLSVYPGKIFDMKNMPGLTMDQVVKSFSTGDVKGSVFKMLQVNDGLAGRVTGIGSAV
                     IGGDPINPNVSATDSNNLKAAASTRIYERARAIEQETMVDLVKKQLSFMAEMYDEELT
                     VKLTDQEFYKFMPGKEADAPLEKVAQFANDGFQGVVFADDIAAGFDVVVEGESTLPIS
                     RSERRTEAMQLMKIATESRRPPTSQELAKDPSLPQKYPQGVPVLDANAVAEDILLPTF
                     TSVDNIDELMWSSDDEGTGAPDRGRGVGRPPDALNPDTMAPAKTEGQIATEGGPQPAD
                     MGVNQMERVDQAA"
     gene            complement(11806..13455)
                     /gene="terL"
                     /locus_tag="PLS35_02275"
     CDS             complement(11806..13455)
                     /gene="terL"
                     /locus_tag="PLS35_02275"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNI17505.1"
                     /translation="MEYTLTQLEINPSMELTDFTVTSFRKQWEELLLEAKKMAEEKFP
                     VDKDAAATEFEGLLFALYEEYAKTNFWFFVTEILGWRGLSREFHGDLMGFLSDAEIKS
                     KLILCPRGHLKSTIGTVAYALWRLFCNPDLRILIVNYKLENAKAFLWELRSIIEMNET
                     VRGIYSRFIPTDTKKMKWNETSILLRRVKIMKEASIEVAGTTTEVTGKHYDLIIYDDV
                     VGPENIGTLEQVDKLRHWFNQVQAVLEPGGEQVVIGTRWHFADLYGFIEENLSEFQSF
                     KREVYKPDGSTLWPEKFTKQAVERIENRMSADPKSGRALFLAQYRNIIVDEQTATFKH
                     SMVKYIEDQPGGLPSDLGVTITCDPAISDKQSADRTAFVVRGVDSSHRWWVLEVVARR
                     GMTPAETIDELFRLYTKYSAKYFVAGVGVETQAYQKSLVFQIRDRMMRDGVFLPLVEL
                     GNWRTTKELRIRGVSPLFENGAIFFRRPGEDDTNILIDELLRFPKAPHDDAADALSMH
                     LEIDTVASPAPSGTEDTEPTGLDRYGYKIQPQTAGIGVCGL"
     gene            13573..14727
                     /locus_tag="PLS35_02280"
     CDS             13573..14727
                     /locus_tag="PLS35_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17506.1"
                     /translation="MGSSIEQHPRSKNYFVEQVFRVNEKLDTFFPPDPETKVNEKLEA
                     LVLVGRIILEETTPDTWDRRGYDLFRLQVRRMKRYDEYSDDALDEVFRKAILLEGEGN
                     TQRFRLYAKNGGQVAKAAESTKPISEAPVSSNPGFVAKILADGKNLLITTEYGLVDTT
                     TERILSFTLFQMDVLRDTHFVYPSIFKKRHEQNVAAWFQSVVEVKKTTSDAEMFNAFF
                     KTAIVDPLSQEAALTQETRPILRNGVYAFKPNTLLDWARENERYQKLSRPEFVSALTK
                     LYGPDFNSGAYRFKDRCWTVTAAAHDTVMGGTGRDKYDDEGNAKADPEVQHDGGGRQD
                     DSTHLDVSGAPGEGHLAGPDPGPLVLEERSGRAEEPPPTAPQPDESGGNG"
     gene            14618..16120
                     /locus_tag="PLS35_02285"
     CDS             14618..16120
                     /locus_tag="PLS35_02285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI17507.1"
                     /translation="MSPDQILVLSFSKSAVEELKSRLRPLLNLTKAGVMDDAEFKEET
                     RMIRTLHSLAYESMKEMGAKLLSTDKFQKFCDEKGWDISPSMGGLFEMRDGGTHDRVW
                     NVLERSRLSGRPIEELTPYAGEVALARRLKDEYDEFKENENVCDYTEMIEFAITNRAV
                     PAGIRAILVDEAQDMCDLMYRYHQVLLQELQEGGFSAWYGDEDQCLYTFMEAEPRFFV
                     EMPAEHTVLGEQSYRVPTRIAALAQSIIEKNRDRLPKIIQGVRGGGEIIDCDSVSSAA
                     DEAMKRDGEVLWLAPTNHQVDMIKAQLLAAGYPVKLSRDLEDAKRAMRLIQDKPKTLT
                     QEELGLLLTAKVGKKLIPLRKDWFVSPRDFNKWYQGTLNGSRIYPPEGLEIATDDRLT
                     DKFKALFNVGAIEGLGLDDRQLKVLASLGESRETGRIQLMTIHAAKGLEAPTVVLCSE
                     ARGKMYEGFIDQPDTLRRLLFVGVTRAKETLIFHRRANTTTRELRKLVGA"
     gene            complement(17034..17564)
                     /locus_tag="PLS35_02290"
     CDS             complement(17034..17564)
                     /locus_tag="PLS35_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17508.1"
                     /translation="MARTLEQKMRVEELRRSFGLTDREAGYAIATVELPHGSSEQDKL
                     RAAGYAEGSARTNKITRKVRTSTKIQKAIEAEARRVARIKELAEEDYEKTKVDAPGHI
                     ERKLWEHQHDATVTPNQTRALELIGKMHAVFVERVDVSAGEHSPAAANLLGRLKMTKE
                     LDKSQEILTGEFTPKE"
     gene            17711..17854
                     /locus_tag="PLS35_02295"
     CDS             17711..17854
                     /locus_tag="PLS35_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17509.1"
                     /translation="MESTKIVSAPLRTLTPKADQYAALKAAYVAQLIGSAPKQNPTHP
                     VWR"
     gene            18129..18728
                     /locus_tag="PLS35_02300"
     CDS             18129..18728
                     /locus_tag="PLS35_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17510.1"
                     /translation="MIYRRSPVTGRALSRFPVAGTVITTLGLITTVVWFATQYKPVEA
                     AHAASPALPLEVIALTGDLPPCTLDTSTGIYDCTDPARTVELHARMQVRYTLPVTRTR
                     YSRADSCHYPSTINGKSVCLTAAGRDTVEGVTVACPRSLKLGTRVRFQGEHHIYTCED
                     RYSRWVDAMRDRPTIDVFAEAAHLSDLPAREDVTLEVLY"
     gene            18816..19028
                     /locus_tag="PLS35_02305"
     CDS             18816..19028
                     /locus_tag="PLS35_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17511.1"
                     /translation="MTYDEACKIDDLLLTTGQKVFTMEDGTLFTREDCKKIIDEFNTI
                     KRKCARCGDELAESDDLICGYHHLED"
     gene            19050..19295
                     /locus_tag="PLS35_02310"
     CDS             19050..19295
                     /locus_tag="PLS35_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17512.1"
                     /translation="MDTLKQSLKESIAKDHARQLSGIVVPWTREEFKKKKTPHGLYAT
                     ILLKDGSRATVSAKRQRDILEAYGENAAMVLECLCDF"
     gene            19791..20111
                     /locus_tag="PLS35_02315"
     CDS             19791..20111
                     /locus_tag="PLS35_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17513.1"
                     /translation="MHMQTFSFDELPYETRVAITSRYYADTAYQAFLGKVVSENPEDF
                     ADVGDWAEETGMRFTFEGAHVSEEAIKAHEKNVKTARVILQAARENGNLSVEACDLLS
                     SFIE"
     gene            complement(20171..20362)
                     /locus_tag="PLS35_02320"
     CDS             complement(20171..20362)
                     /locus_tag="PLS35_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17514.1"
                     /translation="MAAIAAIAASRHRFFMIQEFGLAHPVTNHNVRHFTGEGVVKRVA
                     FLNELYETFNITHRETMRG"
     gene            20575..20727
                     /locus_tag="PLS35_02325"
     CDS             20575..20727
                     /locus_tag="PLS35_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17515.1"
                     /translation="MQSFLFEDLDERGKKAAIFRYWSEVPADWDIEVFFKVAKWRFTE
                     HGERIA"
     gene            20753..22132
                     /locus_tag="PLS35_02330"
     CDS             20753..22132
                     /locus_tag="PLS35_02330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI17516.1"
                     /translation="MDISAPLLMPHQDAGAAWLLAQGAGLLAFEAGTGKTISALEAVK
                     RRDARRILIVMPASLLDQWHSEIIRWGYGEDVYQMTGGKAEREAVYRGLAHPGALVDM
                     APSGRVFILTSYERFRLDAKAAYGVAWDAVILDETLKIQSPTALMTKAVLLMPRLPGQ
                     LRIALNGTPYSNGWGDIWATVSWLAPGTLYRTWWQFRTMHAIVNPYFHGITGWRGVDE
                     IKSKIKPHIMTVRKTDVLSDLPEKTEIQIPVTLSKKEFAVYHRIKEELILEWDGEDTS
                     LPNIIVKMMRLRQCANGLFAFPGHGEVSSKLDVLGDVLEPLFQASLPVVVFSEFEETA
                     NRVAQQYGLPVISGRVSQKDRAQIVEDFNAGKVKGIVGTRAMALGLNLQAASHLVNVD
                     LPFSYAQYDQRIGRVWRHGQKNAVTVYNLMANNTVDFNVWAILARKLSGADELASVTR
                     ADVDEILST"
     gene            22219..22434
                     /locus_tag="PLS35_02335"
     CDS             22219..22434
                     /locus_tag="PLS35_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17517.1"
                     /translation="MSEELKGKLEEAIGGGPEPKQVDLGLLLEAMIEKGGGQIVLAKN
                     GNLFAVCVVSLPFTGELSKQTEPAPAV"
     gene            23772..24260
                     /locus_tag="PLS35_02340"
     CDS             23772..24260
                     /locus_tag="PLS35_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17518.1"
                     /translation="MKKLLVAVSLFATVLGTAPAALAASASENQLIQARIDAMLYAVE
                     NHHPEVSLAADRYNTDLAFVMSANGISREDAIARMARALIAQNGRMPAPPIPSNVGAA
                     YDPAPHAFRQAQARTFYAGVDPVTGTHVFTVEQRASLLDSFWYCTPGKVYTYTPSSSS
                     CK"
     gene            24537..24803
                     /locus_tag="PLS35_02345"
     CDS             24537..24803
                     /locus_tag="PLS35_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17519.1"
                     /translation="MASPNTKKLQEARRLTRLSRVKIVRPAEPGPNRAERRKKIRLRP
                     KMKAERRQLLLDIKNPPRVKPVKKTAKPVQKPTAKRKFFNRRSS"
     gene            25136..25429
                     /locus_tag="PLS35_02350"
     CDS             25136..25429
                     /locus_tag="PLS35_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17520.1"
                     /translation="MMDIARKILMGLVFALFAVASFAILGSRIYGVPFNWCPTKNYFL
                     TITAPAQGAHGNLVYSGQLCSKQNPVEGINASIRKMLNLGEKDGFVIDFMMEL"
     gene            25598..25795
                     /locus_tag="PLS35_02355"
     CDS             25598..25795
                     /locus_tag="PLS35_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17521.1"
                     /translation="MRTSTIIAIIKTAYLAAIGIGLNLYYPDFGVLRTLGVFTIFLGF
                     GMIHHALENQIEYEHFHANDR"
     gene            26276..27724
                     /locus_tag="PLS35_02360"
     CDS             26276..27724
                     /locus_tag="PLS35_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI17522.1"
                     /translation="MMGLGARTSEQRIVVLNTLIAYLLLFANIKDFQWGPKHRAEATL
                     GNPLYYGSYSSISSLLALGLHGWGKLLAGPLALGTILSGSRAPLAATAAGLLTYAASE
                     RRARKPILGALALVAGGAVALKFLQRRDLNPKKTVISRFIVTGDFNARHAFWKAAIKA
                     WRAKPLLGWGLGSYENLFHAYGDPQIIYRSHYETWPDKAHNTYLDKLAESGVVGLGAH
                     LLVRALAIWRATPHARAALVASMVQDAFFFPHPSTAHLDAVLTGRSLGRAGRKRSSLP
                     GLIGSLPLAILTLFGARRIKDEQRAARAVSLLQQGRHAEANESLRRIRSGIDVRPLLG
                     MRLGTETLELLDAILDQTDAENPKDAKRRYIHAGFLDLNNFKKEKAGEIYKCALELAP
                     TKIPILLDYGSYLDSIGDVVGAEAQFAYAKTIAPELGETLWRYGLHLATYGKREKSGA
                     EMLVQAVENVKYKYKPVSSGDIHIYETAKSLL"
     gene            27721..27969
                     /locus_tag="PLS35_02365"
     CDS             27721..27969
                     /locus_tag="PLS35_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17523.1"
                     /translation="MSPSETVKQREYYMSLGRAIFNSVKGYGALVDDSIRHAEKWEKL
                     SAEAQQVFGAAAIIVTGLHEFAKGESALVPNVSITKRS"
     gene            27974..28363
                     /locus_tag="PLS35_02370"
     CDS             27974..28363
                     /locus_tag="PLS35_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17524.1"
                     /translation="MKPLIILSLFVMLCGAGCKKAPEVRATLHQCTPLELQTDELCIR
                     LRKEVEADNLRDLEAKKQKPIMVTVKLKTPIKTWCPAYDAPNGCTEEYKTETTTQIPL
                     QEFVDLLDFKYVPATSTTSNQPAKLVK"
     gene            28366..28635
                     /locus_tag="PLS35_02375"
     CDS             28366..28635
                     /locus_tag="PLS35_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17525.1"
                     /translation="MKPGTLIMLPDGRIGTVVYHGLDGYGIQWGEVIVDEAILKSVNP
                     LFGEEIEDYPYVPQAMLRDPYKNADVECVGSDYNIVTPLTNQQSS"
     gene            28643..29074
                     /locus_tag="PLS35_02380"
     CDS             28643..29074
                     /locus_tag="PLS35_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HNI17526.1"
                     /translation="MRPIKFRVFDTHHKCIRQAHQIDVRISGENVFVNTGFGELTEPY
                     IILMQFTGLLDKNGKEIYEGDIVKQTWQTVVGVLAPNEDEVYETEECITEVVFENGAF
                     CLKNSYHNYGAIPVFEAVDKYTESFVEVIGNIWENPELLTH"
     gene            29112..29378
                     /locus_tag="PLS35_02385"
     CDS             29112..29378
                     /locus_tag="PLS35_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17527.1"
                     /translation="MIKKEPVKKTFGDILREHGAVFSICLSDGGYANVDLNHDGKKWV
                     VTFLNDDGCNREDEIPMEDVEKVARFVQQLRDMRLPDFKTDLVK"
     gene            29543..30037
                     /locus_tag="PLS35_02390"
     CDS             29543..30037
                     /locus_tag="PLS35_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17528.1"
                     /translation="MASALPRNPNMPPTPFPAGKTVRELNIDLKKKFIVVKPDGESRY
                     HGVKEGDIAVATKHWQTDSDCITCKIERPGGQMICTFQLIELAYYEPTEQEVWEPKEK
                     DLVNLKGAVVQVKPNGSLTVSVGGYNLAFLPKDVEFFSRPAPRKVTMAEVCERFGETV
                     IIEG"
     gene            30194..30538
                     /locus_tag="PLS35_02395"
     CDS             30194..30538
                     /locus_tag="PLS35_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17529.1"
                     /translation="MTILSLETSKKVYELCGEYDTERVFAYDFDGRYTVMFREELEIC
                     LGDVPAPNFAELIRLLRKIGEKKGWKQGEFSDAVGVAFSLLMLYMNAPTEPEGMRAVE
                     EYLMKLFYDPRR"
     gene            30522..30767
                     /locus_tag="PLS35_02400"
     CDS             30522..30767
                     /locus_tag="PLS35_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17530.1"
                     /translation="MTPVDKNAIVDMLVRCVNKSLKEFVPTSGRLSEPMIFIDAARFL
                     QALEAEREEIVTDEPDGIALSRYEALKRLSQYPDSIR"
     gene            30838..31125
                     /locus_tag="PLS35_02405"
     CDS             30838..31125
                     /locus_tag="PLS35_02405"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNI17531.1"
                     /translation="MNTFAAQRSICYYCKRQVVFSEWSIDHIIPIVKGGKTTEENCIG
                     TCRTCNNLKGSLPHDYFKAKMESQLSLYESQDLLSRDDSPPDRGHIDDADA"
     gene            32008..32190
                     /locus_tag="PLS35_02410"
     CDS             32008..32190
                     /locus_tag="PLS35_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17532.1"
                     /translation="MEELRASLAEHGRMARARAPKSTERIGKVLQLFDAHPDRKTFTM
                     CVNMLSEIRFDEWISG"
     gene            32311..32736
                     /locus_tag="PLS35_02415"
     CDS             32311..32736
                     /locus_tag="PLS35_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17533.1"
                     /translation="MPDNVYQAQLIDVDLKQNRKFKSDEEETRLQFTFVIIDEGPYYG
                     RRVWEDARQIVSMRPKTSKLYELIKSLTGKTFTEEECKRYTEIVTPEFLNGMIGTQKR
                     LALSTHVSQTGNKSNKIQAIMPVEKELPPYDETKVKNEQ"
     gene            complement(32824..>34158)
                     /locus_tag="PLS35_02420"
     CDS             complement(32824..>34158)
                     /locus_tag="PLS35_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17534.1"
                     /translation="VVMQARASQNPVVSTIAKFTLPFVRTPVNILKQGIEYSPLGIVN
                     LPGAANKSEKIAKILMGIGTTTAAASLLGQDRITWGTPTDKKEKEDFLAAGMQPYSVK
                     IGDKWYSYSKLHPALAFNLALFAALHDANKRGEVDDTMLDKTLSGVAKWGGFFADQSY
                     MKQIGDLMSAAKGDAEAGGKIIANYASQLIPARALMGWVNNIIDPVQRAADTDGSLLD
                     QQMQWIMSQIPGLSKNVPARTDSKGAPIMKSNNILNAFSPVKVSTGNQTGEVRYNERV
                     QANVKYAGERKEQRKEASAQQEKARVEYQRLLKLPKDQANAELDRIKAEDPRLYEDMA
                     TIHKSTVRNYTIDEENLRTLNIENGFRAAHIWAELQKMGSDEEKNAYLQDLEEKKIMT
                     EDVSNQLAARYILEATSKMKTASEKNTFLDGLRKQGRLNKGVEQELRRLSSQ"
     assembly_gap    34159..34258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34259..35616)
                     /locus_tag="PLS35_02425"
     CDS             complement(<34259..35616)
                     /locus_tag="PLS35_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17535.1"
                     /translation="MNKPVQPPGAVQKGIDIVEAPLKKGLQFGQTILQAAPRTVAQTA
                     LGIAGVKAPVAPKTGLQKLVLGNEPIDPQEAYKAGQKAAATQNKITPGSSPLAKLSRI
                     SGIGLGALMMGGLKAADVAMPEGEGAAKKVGQEGIEVIAKGVDKAAPAIEETVNSIKK
                     LATSKPAQEAVAVERLAPKNLFPKELPPVNEEKGLLETFKKGEPAPTPSIQKTESQLR
                     IPEGNTPGGVPSEAPRELSSYSEGVTPSGENVKQTRLDVSEGAKKSVEEGVSVAKPRI
                     EEVVGKKLTNKEVEQYARDTGKIMEETVGRDVTLQRAAENLNLRREIAKVAENGKITP
                     DFIELLVRDRAASADTARQLQARKIMAKPDQLSKIDTVIDAVTKREKDFQKVIDEAKN
                     VDWNDANAITEFYRAHVKPNVGEWIDTLRYNSMLSSPLTHIQNVASNFQGTALVAPIE
                     KA"
     gene            complement(35722..37254)
                     /locus_tag="PLS35_02430"
     CDS             complement(35722..37254)
                     /locus_tag="PLS35_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17536.1"
                     /translation="MMKKDPWSTPYGPPSPTATASAIRKVATPSVSVPRPVAAPAVPS
                     APAAAPAAPSPVQLNAGQMSTQAQLQDRAGLVGSPVDPNISQDAFAGNVAMALRNAGG
                     SPTAGGAPSPSYVPYTPPGASVPPGASAVTSPSDYAKSVATALSSYKPEAVDYTNPVF
                     NGRGIEVTSSNIPVPERSSFASDADFQQAMDTYNYAKLQEESKIANSSAFQYANSQNA
                     QAQEQQLLKMAEQAKASAIEETDRKLRIQRQSLIDAMAARGVTPTTDSQAMAELQRFD
                     QQAQSMRDAAALEASAAFSNKAASVRAQMQADAQARVKNIADAIRQVIDSKAKQTTAD
                     AAYLKSQAASDKNDIDKAYKEGKISLEERDMLLKEAESPANIALKEAQAGKASAEADY
                     TAGAKTANAEANTGYMAARTKEILDLLPYKIDDIKSEIAKRARAGTGGGGVANDGQIA
                     EAYQSFLQQGIEPSKAELANRASVMASTGMSATDVSSLGLEGAGALASAKGSASSGGF
                     RF"
     gene            complement(37380..39131)
                     /locus_tag="PLS35_02435"
     CDS             complement(37380..39131)
                     /locus_tag="PLS35_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17537.1"
                     /translation="MTVSLPITKFNPMPGIFKKTVSFFKTTLAQGISDSGTSLVVNDV
                     PGGAQEYPTWGVIENGSSGAELVYIPTAPVGNTFTVVRGLDPEADADTDAGSSFRRGH
                     SSNVDFIIGPVHRHWNEVVKVMTGVGGTGSLVLRIGDDTDSTITIYAQNADGNKPFVR
                     YDAATNKWLISNDGVNTYDIASGGSGLSAGNGLQIITSVISTNLRSSGGLRNNQGVGS
                     VQMDVDPAIVARLDTANTWAAIQDFRLTSAKVSTPTAAEDAVRKDYVDSRLVSNNYGD
                     GSDGPVDMDGTNTYASFASKSGSTYTLTRDVFATTLRVRVGSTLKMANFHVFANTSIL
                     NEGTMSNDGNAGTAASGETQGTGGAATAVGSMPANIAGGNGASGGVNNAPGNNGSAGS
                     NANHSVSATTSGAGGNGGTSGTNAGGTGGASGTNTGAALNIPRSLPGAYMLFDVNGTA
                     IIALNTAPGAGGGGGGGGSASSVQEPAGGGGGSSGGFGFVAAPDITNTGTIRCNGGNG
                     GAGATGTGSNVGGGGGGGAGSGGWLIIVRRTITNSGTIQAIAGTPGAGGAGIGTGVTG
                     STGGSGSAGVVTQIVSA"
     gene            complement(39100..40638)
                     /locus_tag="PLS35_02440"
     CDS             complement(39100..40638)
                     /locus_tag="PLS35_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17538.1"
                     /translation="MEAYKPVVQQDFSGGWNLRWALNAAQLLENESPYIVNMDYAARF
                     ALTKRRGVKIVGDRNTGMGSIPSLYTYKKYDGTEILIRSRDTILEKLAAGVWTSFDTG
                     FTASQLFDFVTANNIVIYGNAVEAMKSYDGSSVTSYGSNPKGNIMEVGYLKLFVAGVT
                     ANPNRLYYSVTDDYVNFSGGGSGSTDFQAKIVSVRCFFTRDGAESVQVFLANGDIFDV
                     GFDSGGIYKKKVRINCGAVTHRSTKQIENYNFVIDVFKDIRAVGYEESFADLRASERS
                     SPVSPYLLQSDLSLASAIYTRRDYILALKEQGALTNNAELVYSDEYKSWRRYIGHSIT
                     QFAVYGGNLCFASATDPNVYQYDSSTYNDDGVPIYARFDTKGFNFGDPIRSKHGRYVK
                     IAGYISSGCEIGVSLIGDGKYNSPLSQKTISGDGPYVDKSYSLTWGSDQWGGVPWAGF
                     AGYSSNIELRPFWVAVMCSDAKFDELRIMLENYQSDVDFVITYIKPNYVMDADDRIPA
                     DNQI"
     gene            complement(40641..>40966)
                     /locus_tag="PLS35_02445"
     CDS             complement(40641..>40966)
                     /locus_tag="PLS35_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17539.1"
                     /translation="YLRVFSPTQSPLSGSLRIIWCTSDLCVDGTTNETKALPTSDNDY
                     FLIPERFMETVIEGLNKLSFRKDRSNRADQTDSEKAFNDALAEMQLMETIQVDAPVRD
                     MQGAW"
BASE COUNT         9324 a        11374 c        10793 g         9375 t
ORIGIN      
        1 atgcggaggc tccctgaaag gggggactgc gtcggggaaa aaacacggag gtaccggtta
       61 ccaatgatgg agtatgcaaa gcgatcgacg gtaagcgcga aattcttgat gtccatgtag
      121 tccatctcga gcggtacagc cgcatcctgt gagacagggt tggtaataga aatgatgcga
      181 caccagcgtg gaaggagagt ttcaaggtca tattgctgcg cgcctgtaag cgtgaggacc
      241 gtttccttct gctggaaagt atatgcctga ccgcgaccga tcatacgcaa gacccgacgg
      301 agcatacgga gaagttcaga atctccgatg taggattttg tgacgtcacg gaaggcgtcc
      361 atctgctctt ctttgatgtt cccccaggtc gttggcatac gctatttttt cttggatcga
      421 ccggcttcac gaagtgcgat cgcgacggct tgcgcctttg gtcggccgct cttaatgagt
      481 tctttaatgt tatacgaaac cgctttgtcg ctctttcctt tgtcgagagg catattggtt
      541 atgaaaggat taagtgggct gaaccggtga ttccaggata cgccttggaa agctgcttcc
      601 gctttccatc aagcgggtct gagacccagt aatcctcccc accaggcagg acaccgatgg
      661 ctgcaagcca gtgcttgtcc ttcttaattt cgacctggag catggcgacg agcgttgggc
      721 cgccaaggat tgacgttcga atcttggcat cgttacgccc gtagatccgt ccttcacaag
      781 ccttgaccat tcctttcgtt gctttgggaa ggttcgccca aatcatctcg ccctggtcat
      841 tgtaaagacc ggggagcttt gccatctcgc ctggcgtgat cttatagcca cgagagctca
      901 gcagggtgca gagggccgta atcgtacagc cccagcgcca tagcggaagc ttcgtctttc
      961 cgatcgtaac gttggcccag agcgggtcat tctgacgata gtagacaatg ggtgatggca
     1021 tatggttcac gcccagacct tgatagggtc ccggcgtgaa ccaccgaatt cagatcaaga
     1081 gctttatccg tcgctggttg atgaactcgg gtggctcaat ctcgactatt ccgttttcgc
     1141 ttcttccgtc gtttcccccg tggatgcttt gtcagttgca gcggcttctg gcttggcgct
     1201 ggcatcaaga ccagcatcag cattaccagc gagttcaccg atcgcggcat tcaaggtctg
     1261 tgtacccgat cccttgacga tatcaaggac aagactgaaa gcggcgcgac caagaagagt
     1321 tacctcttca gcagagagtg aatcaaattg ggtttcaacg gtgatttcac gctggcccaa
     1381 tgcgagttct tctgacatag gtgtatgtgt tttgaattaa atgggacacc tctattatac
     1441 cttatgaaaa agggaagggc gagccttccc taagagcgga gtaccagcgg ctcgaacggc
     1501 tggacgtccc atgcttcccg cacgtcgcgg gaataccgag agcgcgcccg gttgcgctac
     1561 cccagaaggg tcaaagaccc ttgcgttgtt gcgcgaatcc attgagtcgt ttcttttcga
     1621 actcacggat atcgagcttc ttgagcacgc cgtcggggtt gacgacctgc acaaggattt
     1681 cgagagtggt gtcctgtgcc atcttccagg cacgggcata ttcaatcggc gtgatgagta
     1741 ctgccgcggt gcacccgtcg ttcaggacga agtgcattat gcggcgctcg gtggccatga
     1801 tgccatctct cctttcggcc ggaagttgag ttccttctcg gcctctctca aggtgcgttc
     1861 gacatctacc ttctcgatgt gaaacgagaa cgggtagcga ccgattttga cgttgatcct
     1921 gaaccaggaa atgttcccag tctgaacctc ctcgacgatg tgcttgaacg acttgagcgt
     1981 catggtgttt tcaccacgaa acccatcagg attttctttg gaccgcttga accaaatgtg
     2041 cattgcttcc tccctcctcg acgcgagatc gagaaggaag acagtttccc ctccttgcgg
     2101 agaggaggga ggaagcactt ttttatggtt gcgtcttttc aagggccgtt ttgatttcct
     2161 gaatccgatc gaggattgta tcgagcttca cgtcctgaac tgattgtttt gtcgaaagcg
     2221 cctgttgaga aacatcgatc gtgttgattc gcgatgtcag aacctcaatc tcttttgagt
     2281 tttgggttga cgttgatgcg attccagtaa cgtacacaat ccctccaccg ataaggctga
     2341 tgataacgac aaccgctgca acggctgcac cgatgggaag gactgcgcgt gtaatttcga
     2401 aagggcttga ttcagacata tctcaggctt gtggcccctg gaaattgtcc agattcacgt
     2461 tcgcgttttt aaggacttct ggcgatacgc ccttcataaa ctgccatgcc gtattcacaa
     2521 ttgaagtact gagggcgccc cagaccgcga aggcagctga gcgccaatcg atcgcggttc
     2581 cctgaaggat gaattgaccg atgtccttag aaaggacgac gaatcccgct aataccgccc
     2641 caccaaccga gataccgata ccgatgagaa ttttcttgat tgtctcttta tccaaccgcc
     2701 atgcaatctg catacaggtt attgagtgag taaatctttg agctcatcct tcaaggtgtt
     2761 ctgctcatca agcttgatac tgagaccgcc ccgttccttg atcgccgcgt cggcgatcgc
     2821 ggttaccgcc tggagatcct gccaagtcat gcctcctggc ttgtctaaga gcgccgcggc
     2881 ttccgccttc agcgtggcgt aatccgtttc ggaatacgcc acgcagttct ttccatctac
     2941 acacacctgc aacgtgcctt cagtctttgg atctttccca tcgaagacga acgaaaccag
     3001 gccgccgatg gccacgatgg acgcgatccc gatagctgca cccgccttct gaacgtcttc
     3061 tttatccata gaattgaggt tattgcttag tttcccatcc tgaaacgaat gtgaatgtcg
     3121 gagttccgct aaccgtgact gaacggagtt ttacgtaata gcctgcggga attggtgcac
     3181 tgagcgaaca gccattccct tgtgtgctct ggactgcaag gagtcccacc gtattatccg
     3241 cgcggcaccg agttccaact tcgtaccaat cggctgcatt cgaagagttc gtcggagcga
     3301 tctccatcgt gatgtatcca gccgccggcg cgccaagcgt tgcaaccgtc gcaatgttta
     3361 ccgcgtatga aacggtaacg ttttgcgttg ctgatacctg gaaccctgtt gcacccgttc
     3421 ctgtcgtaag cgatcgggat gaggtagcga atgtccagga tgtacttggc ttgttaagga
     3481 ggtcattgta atcacctgtt gtcgcaaccg tcgcgagaga tgctgcatcc gccttggcag
     3541 cgagatctgc cgtaagacct gagatatctg cctgatcgag acctcctact accacttctc
     3601 cagttgacgt gttgttcgag aggccatgtc ccatcgtgaa tagaaatggg tgctgtgtat
     3661 ctccacgcat cccgaggatc tgacttcgac cgtccatcgt cggttggaaa tagtatggct
     3721 ggaacaagaa gtttccttca tcccattgcg taatggtcag gtccttgtca gtgagcgcgt
     3781 aggcagggaa tacagctgat gagatgacga ggagcgaaac gactgccgaa ataccgaact
     3841 tttttgcaga gttctctagg aatttcatac tttttgtttt tatttttttg actgatgcta
     3901 tacatgaccc acctcctcga actatcgttc gttgatgatg agtacattta ccttcgtatc
     3961 agctgtaggc gctgctggag cacgcacacg gatctgacca accgcagttg ttgttacctg
     4021 cgcgtaaaac atcgtcgcat ccagatccgc aatcgttact tggacgtgcg ttgatgtggt
     4081 tccacgaacg aatgaatcgt tgatgatgac agatgttgcg gctgccggga atttgaacga
     4141 gaagtttgtg gcattgatcg tgtagttggt cgacgtggcc tgcgtcgatg tacccgcgat
     4201 tgcaatacct tgggaatagt acgcgtcagt acccgtcgta tgattgaacc aaccttggcc
     4261 gtacacacgg atcgaactac cgacgttccc tgtaccagtc gagagaggca cgacgtttgt
     4321 tgcaaaccag ccttcacctg gatctaggaa tgggccggtg ttcccgttcg tacagaactg
     4381 gatttgatgt gaagcgttga tgtagaaacc agtaccctgc gaagcaatac caacaacacc
     4441 gccggtaaga gaggcgcagc caccagggga gttgccgata tacatctttg tcgatgtgac
     4501 gctcgtggaa ctaacgctcc cagcaaagag gttccaagga agcgtcaacg aaccgaggtt
     4561 gcgcgtggtg ttatccggca agatgttccc gtagaagctc gatgtaccac ctacgttgtc
     4621 gatgttgaag tagagctggg caccgcctgt accagcagaa attgccatcg cgcccgtgtc
     4681 agtacgaata caagctgttt gggatgcggc gcagttgagg ttggagtttg taatgttgat
     4741 cttcccggag tttccaacct tcgacgtaat cgtcatccaa gaggttgtag cgctggttga
     4801 tgaaacgctt tgcgcttgaa gcgcaccgga acctgtggca ttggtgaaga gcaaccgtgt
     4861 cgcaaagagg ttggttgagg tcgtattcgt gccggtggca ttggtccaga taaggttgtt
     4921 tgtgttgatc gttccagaca catacgcgtt cgttccgaga taaaggttcg cccatgtgcg
     4981 cgtcgcagaa ccaagactga acgtattgtt cgccgaaggg agcatgtcgc ctgtaccaat
     5041 tccagaaatc gatgcggttg cggcggagag gaatgtgaag tacccagctg caattgggtt
     5101 cgtcgcagag ccaatcgtgt atgctggatt cgtcgtaagg atcgcaccgt tcttgagata
     5161 gaagagaccc ggctgcaaga cgcctgttcc acccccagga gagagtgtct gggagtttgc
     5221 atactgaacg cctcctgcga tcaccatgat agcgaccgag aaaagcgtga gcagtgagta
     5281 gagaattttc ttcataggtt acgtgcgcgc ctggaggtcg cggatatcca tgaggaacat
     5341 gttcaccgtg ttcgcgtcag cagcgagcat gatcggaagc ccaaggttcg tcggcatggc
     5401 cgaaaagcca tccaaaagcg tcgagacttc agcgaccttc acgccattga taagccagcg
     5461 tacgccggtt tcacgccaga cgacctcaaa cacggttgtc gtgttgttgg tccaagaagc
     5521 gtcccacgtg atcgtggtcg atttgataag cgtgcctacg ttgtcgtagg ccaccgcctt
     5581 gaatacctgg ttttcaatga tgaaccccgt tttcccacga ttgccgagag agacagagcc
     5641 gaatccccag aaggagttct gagccgcctg cggatcaacg gggagcttga ggttcagcaa
     5701 aagaccagca taccgatgcg agatatatgt ctcgatcgtc gtgttggtga ggacgatgcc
     5761 gtccgccgtg actgacggat cagatgcacc gacgacggtt ttccagtact gggggttata
     5821 cccagacacc tgtgggtcgt acaacaggtt atcgttcttg tcagtaaggc ggttctgcat
     5881 aagttgggta aattacgtag tcgcgacctg cggctcacct accggtggcg ctggaggttc
     5941 agctggagct ttctccttgg cgctctttcg gatgttcaag cgaacctgca agccatcgtt
     6001 ctctgccttg agactctcgt tctcagcatt taggcgagcg atctcttcat ccttgtcttt
     6061 cagaaccttc gaaatatcga ttggttcacg atagacccag aatgactgcg gcgccttttc
     6121 gatgacagag aatagcttat gagccttcaa ccattcgatt tccttctcgt cttcagtctc
     6181 atacacaccg ttcgtgaacg taatgtactc acctgcatga cggattgctg gcgcgccctg
     6241 gaccggttcg atccagtcgg cacgcttaat caaacgaagg tttttcgcac tggattggaa
     6301 tttcatgtgt gggactgtac gccttcccac atgggcgtcg tgacatgctg tggaggcggg
     6361 cacgacgccc aaggcgtact gatcttaagg attagttaag aactttctgc gtacttacga
     6421 cgttcatgtt cgtgacagcc actgcgccac gagcacggac gaggagacgg cgacccgggc
     6481 gggcgttgat gccaatgccc gagctgttcg tgaagtcgac agacgaagcg ttcactggga
     6541 actggacagc gatcaaacgg ctgtagatga cttcatcagt cttctcctga tcgttcactg
     6601 agatcacctc gaacacacca gcggtgtgtt gaagggtcat tgcgaccaag tgaagggcgt
     6661 tcactgcgat gcgcttcatg tccgtgtcgg cagccgactt gttcatgagg ccatcaaggc
     6721 acgagatctc tgcggtgatg tgaccggcaa ccgaggtgtc aacgatgagc ggagcgccgt
     6781 ttgtcgactt ggcctgttgg ccggtcgaat cagcgacaag agcgcccgtc gtcgaacatg
     6841 ggtctgaacg gcggagaccg atcaggcgcg cgctgtagcc cggaaggtta ttgatgaagt
     6901 caacgacttc acccatcgtg ctgaagcctc cggcgttcaa ggagatggtc cctggcgtcc
     6961 cacccgacgg gtaggtaaag gcggcatcag ctgcttcaga gccagcagcg ccgatcaagg
     7021 acaagatcgc gttgttcccg ctcgtcttga tgagagcgga agcggacgat cccttgtaca
     7081 agatgcgcat cccaaggaac tggcgaatgc tcgagtcatc accggtcgcc acacaggaac
     7141 gcttttgggc gagcccgttt gctacggcaa gagtgtctgc gttcatatga gtgcagttac
     7201 ttgttggtga attagaggac gttcaacatg acgccatggg ctgtctcgtt ggcgaggtgg
     7261 agaccacatt cgccgagcca ttcacccttg taaccgtcac ggtcattttc ctggatgttg
     7321 tcgtgataca tgttctcagc ctggtcaagg tgagcatatt tcacagaagc tgggtcgata
     7381 acgagcatcg tacccgcatg cttctgcgcg atgccgccct taccgaacat cggatggcga
     7441 acgagcttca acacaccgtg gctcgtaaca agctccatga cgttcgaacc gaacatcttc
     7501 gtttccggtt cataacggat gaggttgtcg tagagaccat tgatacgcga gaggacgcgc
     7561 ggggagcaga acgcgatctt ctccgtggag ccacgggcaa agacgagttc agcgaacgcg
     7621 tcgaccgagc cctgtgtcat gacaccaccc gaaccagaga ggtccatgac gtttgaagtg
     7681 aggagcgaga acacgccacc cgtgaaacgc tggcgcgtgc cagaaccatc aacagcagca
     7741 cgctcttcgc ggccaccaaa gaggaaggca cgttcctgcg aacggaggtg ttcgataccc
     7801 gcctggcgct tcaaccgatc gaggtcggac gtcggggagt agttcacctt cgaatccttg
     7861 tccgtacggc cgatctgaac cggcgtgcgg aagatctggg tgtagttgta attgcgcgtc
     7921 ggaacggtca tcttcgccgt gccagagagc gcgttaaccg cgtaagcgga cgcaaggcgg
     7981 acgagataat cggcgttcac aagagccgcg gccgtcgaac cgacaccacg gacaaccgtg
     8041 agggtgttcg ttgtgaggtc ggacgccgta accacaaaga cttcagacgt gcgcgggacg
     8101 aggacctggt cgttcttcga gaagatcgac gcatcggtca caacgatcga ggtcgcggaa
     8161 gccgaataac cggcaaccaa gttgacggtc gtcgtatctt cgagaacttc atcttcgaag
     8221 atctggtatt ccgcattcaa caccttgcgc ttcgagagcg cctgtccaga aacattgact
     8281 tctccaccgt acatttcacc gatcggccct tcaacgccgt acggattccc accgtacaca
     8341 cgaccattca tgccaaggaa cgtgatgaac gggaagtcca tgcgctcctt gttccaatac
     8401 aagcggcctt cgccatcaat gtatcggcca tttactggga ggtcattcga tccaactgga
     8461 ccctgaatga gttctggcat agccaagagt taagagtttt tagaggtaag acgtgcccct
     8521 tccgttatcc acggaagaga cctgcgaaga agtcatcgcc tttcttggcc ggcttgccgg
     8581 ccgcacctga aggtgcgccg cctgcttggg cctgcttgat cttggtcttc tcctcttcag
     8641 tatcgtcctt ctttggtggt tcttgtgatg gacggaccgg ttcctttttt gtgaggagac
     8701 ggtatgcgtc gtagaggtcc acctggagtc cggaactgcc gagcttctca acggcagaag
     8761 ccaggtcgtt cgcaattgcg agcgcatctg gattcgcctg catgaattca gctacaaggt
     8821 cgtttgcgct ttgctgctct tgggagcgga tgcggtttac gacgggactg atatcactga
     8881 gacgttcgga aaggagcttt tcaagatctt cgcgagaaag gaaaactttt ccgagcttct
     8941 gtaagacagc cttttcctca tcagagaatt cctctgcggg cgtatttccc ccgtctttcg
     9001 aatcggtctt ctgcggctgc ttgccgccct tgagctcttc gatttccttg cggagattga
     9061 agttctcgcc gatgacgcgc gacgcgtcgc cgatccgttt tttagcgtgt tcaagaaatt
     9121 cctccttcgt cttgaacttt acatcgcctt caacgtggaa atactcgttg tcatcctgct
     9181 tgcccgcgtc gggcttcaca gggtcgacaa ccttttcgct gcctgcctca ccgtctttcg
     9241 ccggttctgt tggggtagtc tcgccagacg tcccttcagc tccgggtaga ttggccgtgt
     9301 cttggctatc tgccatacgg atagaatgtt cgtgacttcg ttaaagagac cgtttatggc
     9361 ctcgagctgc ggagaacttc tcgcaagtcg agacctacga agccgaacct tctttcggtt
     9421 ggtcaagtgt caaagatgca tcgctgatcc aggattccat ctgctcctta aaccggttaa
     9481 tgaactcctg ccgcccatgc gccatccagc acaactcccg cgccttgtcc ggaggaatag
     9541 aggtgagcgt gagcttctcc atttcctctt gcaaggatac caaaaattgg tcgaccgtaa
     9601 cagaccactc tggtgagttc atgagaatca caaacgaacg accgattttg atacgctcat
     9661 tgagctcttc ttgtgctttt tgttctggcg tcatggtagg cataagaaat tacgcggctt
     9721 gatcaacacg ctccatctga tttaccccca tatccgctgg ttgcggaccg ccctctgtcg
     9781 cgatctgccc ttcggtcttc gcgggtgcca tcgtatctgg gttcaacgca tcaggcggac
     9841 gaccgacacc gcgcccgcga tcaggcgcgc cggtgccctc atcgtccgaa ctccacataa
     9901 gctcgtcgat attatcgacg gatgtaaagg tcggaagcag gatgtcttct gcgaccgcgt
     9961 tcgcatcgag gacaggaacg ccctgtggat acttctgtgg aagggatgga tctttcgcga
    10021 gctcctggga ggtcggcgga cgacgggatt ccgtcgcgat cttcatgagt tgcatggctt
    10081 ccgtgcggcg ctccgaacgc gagattggga gcgtcgattc accttctacg acaacatcaa
    10141 acccagcggc gatatcgtcc gcaaagacaa ccccctggaa cccatcattt gcaaattgag
    10201 caaccttctc aaggggagca tcagcttctt tgccaggcat gaacttgtaa aactcctggt
    10261 ctgtgagttt taccgtgagc tcctcatcgt acatttctgc catgaatgag agctgtttct
    10321 tcacgagatc aaccatcgtt tcctgctcaa tcgcgcgagc acgttcatag atacgcgtcg
    10381 aagcggctgc cttgaggttg ttcgaatctg tcgccgagac gttcggattg attgggtccc
    10441 cgccgatgac agcggaaccg atacccgtaa cgcgccctgc gaggccatcg tttacctgga
    10501 gcatcttgaa gaccgagcct ttcacgtcac ccgttgagaa tgacttgacg acctggtcca
    10561 tggtaagccc cggcatgttc ttcatgtcga agatcttccc aggatagacg ctgagatcag
    10621 ccgggttctt gagatatgcg gagttcaccc cgaacacgcc ggccgtcgtg aacttcgttc
    10681 catcgagtgc aagcgtatgg agtgcgttcg cttcccactg gaggtaccgc agtaaatgtg
    10741 gttcaccgat gccccatgga acacgcgaga ccttatagtc acgcatgacc gtgatcgggt
    10801 tctccttcgt atcctcaagc ggatttggat gatgctcaac gacgcgaccc gcgacaacga
    10861 cggtccactc atcattctcc tgatcgtaga tcgtgagacg ttccgacagg ttctcgctct
    10921 tcagctgcgc atacggctgc gccttacgcc caccgagcgc gtcacgcgtt gatcccggat
    10981 accgctgcat cgagtagtag tcatacatcg gatccgtttt gaagagacac tcaaggtcat
    11041 ctgtcaagtc ccctcccgca cagagcccct tgtaaacgtt cttatacgca agctccggat
    11101 gcgcttcgta atacgctttc acgttccgcg tatctacgag gtcacgatag atgtagaacg
    11161 gccaacggga cggatcgtcc tcatggaccg ctggcaagta gacgcagagt ggatggtcgt
    11221 tctggggaac ccagcccttg taaaacactt tctcgcgctc ttcatagacc gggctcttcc
    11281 cctcctcgct acggtcaaca cccttcacaa atttcatctt ccgtcgatcg tagcggaaca
    11341 cactacggag gaatgatgtc ccgaagatca aagactcctg gacgctgttt gaaatagcca
    11401 ccatcgcttg cgcgcgtttc atctctccct gagagatctt cgaaacgaag ttcgctttct
    11461 cgcggtcctg tggggtgttc tggggagaaa ggcggtagcg gggaggacgg ccgttcaact
    11521 gggcctggat cgaggcaact gtctggaaag tgaccgggac atagatcgct ggaccatact
    11581 tcgcgatgtc gcgctgagcg aacgcccaat cagaaaggat ctggttaaaa gaaagatggg
    11641 acgcttccat catctgcttg atcggggcct tcgctgcttt cgaggccccg aactgctcgt
    11701 ccttgcgctt gaggatctcc tgctcctctt ttgtcatctt gacctgtttt tcagcaggcc
    11761 cacgttggat gttttgaaaa attggaagtt ggtccatacc gggtattaaa gaccacagac
    11821 tccgatgccc gccgtttgcg gctggatttt gtacccatat cgatcgagcc ctgttggctc
    11881 cgtatcctct gtgcctgacg gagccgggga ggccaccgta tcaatctcaa ggtgcatgga
    11941 gagcgcgtcc gccgcatcat catgcggcgc cttcgggaaa cgcagcaact catcaatcag
    12001 gatattcgta tcatcttccc ccggacgccg gaagaaaatt gccccgttct caaagagagg
    12061 ggagacacca cggatgcgga gttctttcgt cgtccgccag ttgcccaact ccacgagtgg
    12121 aaggaacaca ccgtcacgca tcattctatc acggatctga aagacaagcg acttctggta
    12181 tgcctgcgtc tcgaccccta cgccggccac aaaatacttg gcactgtact tcgtatacaa
    12241 acggaacagc tcgtcaatcg tttcagcggg tgtcatgcct cgacgggcca caacctcaag
    12301 aacccaccac cggtgggacg agtcaactcc gcgcaccacg aacgccgtgc ggtcagcaga
    12361 ctgcttgtcg ctgatagcag ggtcacaagt gatcgtgacg ccaaggtcac tggggagacc
    12421 tcccggctgg tcctcgatat acttcaccat cgaatgcttg aacgtcgccg tctgctcatc
    12481 cacgatgata ttccggtact gcgcgaggaa gagcgcgcgc ccactcttcg ggtcggcgct
    12541 catgcggttc tcgatgcgtt caacggcctg tttagtgaac ttctcgggcc aaagcgttga
    12601 cccatccggc ttgtagacct cgcgcttgaa tgactggaat tcactgaggt tctcctcgat
    12661 aaacccatac aaatcagcga aatgccaacg cgtgccgatc acgacctgct caccgcctgg
    12721 ttccaaaacc gcctgcacct ggttaaacca gtggcgtagc ttgtcgacct gctcaagcgt
    12781 tccaatgttc tcaggcccga ccacgtcatc atagatgatg aggtcgtagt gtttcccggt
    12841 cacttccgtc gtggtccccg ctacttcgat cgacgcctcc ttcatgatct tgacacgacg
    12901 gagcaagatc gacgtctcgt tccatttcat tttcttcgta tccgtaggga tgaagcgact
    12961 gtagatacca cgtaccgttt cattcatctc aatgatggac cggagctccc acagaaacgc
    13021 cttcgcattc tcgagcttgt agttgacgat caagatgcgc aggtctggat tgcagaagag
    13081 gcgccagagc gcgtacgcca ccgtcccgat cgtcgatttc aagtgaccac gcggacacag
    13141 gatcagcttg cttttgatct ccgcgtccga taagaagccc atcaaatcac catggaactc
    13201 acgggagagc ccgcgccatc caaggatctc tgtcacgaaa aaccaaaaat tggtcttcgc
    13261 gtactcctca tacagagcaa acaagagccc ctcgaactcc gttgccgccg catctttatc
    13321 cacagggaac ttctcctcgg ccattttttt agcttcgagg agcagctctt cccactgttt
    13381 cctgaatgaa gtgacggtaa aatcagtgag ctccatggag ggattgattt ctaactgagt
    13441 tagagtatac tccatctcag ttctccgcac catgttttgg aacgcccgtt caacctgtcg
    13501 cgtcctcgga cgtacagaca gagaactgtt gagcgggctt tccagcacat ggccccatgg
    13561 ggaaccgtcc ctatgggatc ctccatcgaa caacaccctc gctcaaaaaa ctatttcgtc
    13621 gaacaagttt ttcgcgtcaa cgagaagctc gacaccttct tccctcccga cccggaaacg
    13681 aaggtgaacg agaagctcga ggcgcttgtc cttgtgggac ggatcatcct cgaagagacc
    13741 acgccggaca cctgggaccg acgcggatat gacctcttcc gtctccaggt acgtcggatg
    13801 aagaggtacg acgagtactc tgacgacgcc ctcgatgagg tcttccgcaa agcgattctc
    13861 ctggaaggag agggaaatac tcaacgcttc cgactctacg ccaagaacgg tggccaggtc
    13921 gccaaagcag ccgaatccac aaagcccatc agcgaagccc ctgtaagctc gaaccccgga
    13981 ttcgtcgcca agatcctcgc agacgggaag aacctactaa tcacgaccga atacggtctc
    14041 gttgatacca cgacagagcg catcctttcc ttcacccttt tccagatgga cgtcctccgt
    14101 gacacccatt tcgtctaccc ctccatcttc aagaagcgcc acgagcaaaa cgtcgccgcc
    14161 tggttccaga gtgtcgtcga ggtcaagaaa acgacctccg acgccgaaat gttcaacgcc
    14221 ttcttcaaga ccgcgatcgt cgacccactc tcccaggagg cggccctcac ccaagaaaca
    14281 cggccgatcc tacgcaacgg ggtctacgcc ttcaaaccga acaccctcct cgactgggcc
    14341 cgtgaaaacg aacgctacca aaaactctca cgtccagaat tcgtctcagc cctcactaaa
    14401 ctctatggac cagacttcaa ttcaggcgcc taccgattca aagaccgctg ctggaccgtc
    14461 actgccgccg cgcacgacac cgtcatggga gggaccggcc gcgacaaata cgatgacgaa
    14521 ggcaacgcca aagctgaccc ggaagtacag catgacggcg gggggaggca agacgacagc
    14581 actcatctcg acgtatctgg agctcctgga gaaggacatc tcgccggacc agatcctggt
    14641 cctctcgttc tcgaagagcg cagtggaaga gctgaagagc cgcctccgac cgctcctcaa
    14701 cctgacgaaa gcgggggtaa tggatgacgc cgaattcaag gaggaaacgc gcatgatccg
    14761 gacgctccac tctttagcct acgagagcat gaaagagatg ggagctaagc tgctctcaac
    14821 cgacaagttc caaaaattct gtgatgagaa gggctgggac atctccccct cgatgggagg
    14881 cctcttcgag atgcgtgacg gcgggacgca cgaccgcgtc tggaacgtgc tcgagcgctc
    14941 acgactcagc ggcaggccca tcgaggagct taccccctat gccggggagg tcgccctcgc
    15001 gcggaggctc aaggatgaat acgatgaatt caaggaaaat gaaaacgtct gcgactacac
    15061 cgaaatgatc gagttcgcca tcacgaaccg ggccgtacct gctggcatca gggccatcct
    15121 cgtcgacgag gcacaagaca tgtgtgatct catgtaccgg taccaccagg tcctcctgca
    15181 agagctccaa gagggaggat tcagcgcctg gtacggggac gaagaccagt gcctctacac
    15241 cttcatggaa gccgagccgc gctttttcgt cgagatgccc gccgaacaca ccgtgctcgg
    15301 cgaacagagc taccgcgtcc ccacacggat cgctgcgctt gcgcagtcga tcatcgaaaa
    15361 gaaccgcgac cgtctaccta agatcatcca gggcgtccgt ggaggcgggg aaatcatcga
    15421 ctgcgacagc gtgagctccg ctgccgatga ggcaatgaag cgcgatggtg aagtattgtg
    15481 gctcgccccc accaaccacc aggtcgacat gatcaaggcc cagctccttg ccgcgggcta
    15541 tccagtgaag ctcagccgag acctggaaga cgcgaagcgg gccatgaggc tcatccagga
    15601 caagcccaag accctgaccc aagaagagct gggcctcctc ctgacagcca aggtgggcaa
    15661 aaagctcatc cccctacgta aagactggtt cgtctcccca cgtgacttca acaagtggta
    15721 ccagggcacc ttaaacggct cacgtatcta ccctcccgag ggcctcgaga tcgccacaga
    15781 cgaccgcctg acggacaaat tcaaggccct attcaacgtc ggcgccatcg agggcctggg
    15841 tctcgatgac aggcagctca aagtcctcgc gagcctgggg gagagcaggg agacagggcg
    15901 gatccaactc atgaccatcc acgccgccaa gggcctcgag gcccccacgg tcgtcctatg
    15961 ctccgaagcg cgtggaaaga tgtacgaggg cttcatcgac cagccagaca ccctacgtcg
    16021 gctcctgttc gtaggagtga cccgagctaa agagaccctc atcttccacc ggagggcaaa
    16081 cacaacaaca agggaactac gaaaacttgt tggtgcctaa aagactcccc cagccgggga
    16141 gtctttttat cgatgacccc gtaagcccag caaactgcac tcgaaccccg ggaaaaaggg
    16201 atcaaacgga accaaaacaa cactcccact aaataaaaca acatcaaaca acaatcaaac
    16261 aacattcaca tttggctagg ttgagccaat aaattacaaa aacaacagaa acaacacttt
    16321 ttacagaaaa atgttgttgc aacgttgtcc aacgattttt agccaaaaaa ttgacccact
    16381 ttaaaaagtg ttgtttctgt tgttttggat aaaaaagatg ataaaaagtt taaaattttg
    16441 ctaggttggt agattttaaa aacaacattg atatgttgtt tacttgttgc tcaacaacaa
    16501 aaaaacgact tagtaacgaa gctacagggg ggttgtataa gcacgaatga aaatggggtc
    16561 cctctctaca gggggagaaa aaccgtgcaa cagggggatg ttggaaatga gggggagatg
    16621 ttgcaggggg agatgaggga gccggaggaa gacatgaggc tcagaagaga ggaacggggg
    16681 cagggctggc tgggggagta gggggcgttc ttcccactca atgggaggaa agggaaaaag
    16741 atactcgcgc caaaaagccg gagcgaattt tcgcggtggc gggagtcact gggcaaggtg
    16801 ggggaaattg ttacccgggg gcacccccac cctccccctc ttggcctcgt caggggcaca
    16861 tctggaggcc ttgatatata tcctccccat ggtgtgcccg ccctccctct accctttttg
    16921 tcgggagcgc atataaaaat cccccgtatc tataggggat tactgcgtcg tataatgatc
    16981 gttatacgac gcatggttga ttcttcgcta gtcttagcca aaaagctagc gacttattct
    17041 tttggggtaa actcgcctgt caagatctct tgacttttgt caagttcctt tgtcattttc
    17101 aagcgtccca gcaaattcgc agccgccggg gagtgttctc ccgcacttac gtccactctc
    17161 tctacaaaca ccgcgtgcat ctttccgatc aactccaacg ctctcgtttg gttcggcgtt
    17221 accgtcgcgt catgctggtg ctcccacaat ttcctctcaa tgtgtcccgg cgcatccact
    17281 tttgtttttt cgtagtcctc ttccgccagt tccttgatcc tcgccacccg tcttgcctcg
    17341 gcttcgatgg ctttttggat tttcgtgctg gttcgtacct ttcttgtgat tttgtttgtc
    17401 cttgcactcc cctccgcata tcctgctgcc cttagcttgt cttgctcgct gcttccgtgt
    17461 ggcagctcta ccgtcgcaat cgcatatccc gcctcgcggt ctgtgaggcc aaaagatcgt
    17521 cgcagttctt ccactctcat cttttgttca agtgttcttg ccatgccttt attctaactc
    17581 aatgaaactc ccctgtgaat atctcccctt ttccccagca tttccggcct cttgacattc
    17641 ttcccattga gtgggatgat ggttgtgtca ggagcgcagt ctcgccctcc tgtcaacaaa
    17701 ggatcgtctc atggaatcca ccaagatcgt gtccgctccc cttcgtacct tgacccccaa
    17761 agccgaccag tatgcagccc tcaaggccgc ttacgtcgcg cagctcatcg gttccgctcc
    17821 caagcagaac cccacgcacc ccgtgtggcg atgagcttcc cctgttccgc tcccacttcc
    17881 attgtgtgct tactctcatg tgatggaagt gccgcgaggc tcccctgcct cgtgtctatc
    17941 ttggacggcg cacacatcat gcctttcgtg tgtgccctct catgatggat tgaaccggca
    18001 caggcagcgc ggggggtctg gtacgtggcg tgttcacgcc gcctactcga ccccctcgcg
    18061 cctcgcctac tcaataaaaa taaaaagagc ttgctcaaaa aaaacaagca agctcacaca
    18121 tctcatctat gatctatcgt cgctccccgg taactggtcg cgcgctctcg cgcttccccg
    18181 tcgccggcac cgtcattacg acgcttgggc tcattacaac cgttgtgtgg ttcgccacgc
    18241 agtataagcc agtcgaggca gcgcatgccg cctcccctgc tcttcctctc gaggttatcg
    18301 ccctcacggg tgatctccct ccgtgcactc tcgatacctc aaccggcatc tatgattgta
    18361 ctgatccggc tcgcaccgta gagctccacg cacgtatgca ggttcgttac accctccccg
    18421 tcacgcgtac ccgttactcc cgcgcagact cctgtcacta cccgtcaacg atcaacggca
    18481 aaagcgtgtg tctcacagcc gcaggacgtg acacggtcga aggcgtgacg gtcgcctgcc
    18541 cacgctccct caagctcggc acgcgtgtcc gcttccaggg tgagcaccac atctacacgt
    18601 gcgaagaccg ttactcgcgt tgggttgacg ccatgcgtga ccgtccaacg atcgacgttt
    18661 tcgcagaggc cgcccatctc tcagacctcc ccgcccgtga agacgtgacc ctcgaagtcc
    18721 tttactaaga accttgacgc cgtgaggcca cagcttccac cgcttggcct catccgtcag
    18781 cctcccgctc taagagaggc ttttcacacc caactatgac atacgacgaa gcatgcaaga
    18841 tcgacgacct cttgctaaca actggtcaaa aagttttcac catggaagac gggacgctct
    18901 tcacccgtga agactgcaag aaaatcatcg acgagttcaa cacaatcaag cgcaagtgtg
    18961 cacgttgcgg cgatgaactc gcagaaagcg acgacctcat ttgcgggtat catcatttgg
    19021 aagattgaca aagaaatttc gcaataatta tggataccct aaaacaatcc ctaaaggaat
    19081 caattgcaaa agaccatgcc cgccagctct ctggaattgt cgttccttgg acgcgtgaag
    19141 agttcaaaaa gaagaagact cctcatggcc tgtatgcaac catcctactc aaagacggaa
    19201 gccgcgcaac ggtatctgca aaacgccaac gtgacatttt agaggcgtac ggtgaaaacg
    19261 cagcgatggt actcgagtgc ttgtgcgatt tttgacgccg tgcgcctgat ccagcatcgg
    19321 gcgcatccgt cagctctcca gctctaagga gagcatgaca aagaacaata tgaccagttg
    19381 ctcgtgcgct ccaatcgttg atactccaaa gcttctaaat ctgaaagaaa agaagcgcca
    19441 agggtacacc ctaccaatct attcaatggc ggcgacgtgc ctatccggcg cgtcccgctt
    19501 ctgtccatgc agcaagcgct cggtgattct tgccgaaatc gcagcagaca taaagaattc
    19561 ctaacttacc cttccatgat caaagtctct cttatcggca aggttggctc actcgacgcg
    19621 cgagacgcac cgacctgcct catctctcaa gactgccgtg tccttcgcgc gagtttcaac
    19681 acagcctcaa aatctcccat cttaggcgct gaagtcaaag caagtgggta cctctcattc
    19741 actcaagaca atttgcccgt cctcacagtc gaggaatggg cgaccatctg atgcatatgc
    19801 aaaccttctc tttcgacgaa ctcccttacg aaacccgcgt agcgatcacg agtcgctact
    19861 acgcagacac ggcatatcaa gcgttcttgg gaaaagtggt gtcagaaaac ccggaagatt
    19921 tcgccgacgt tggagattgg gcagaagaaa ccggtatgcg tttcaccttc gaaggtgcgc
    19981 atgtctcgga agaagcgatc aaagcccacg agaagaacgt caagactgct cgcgtaatcc
    20041 tccaagcagc acgtgaaaac ggcaacctgt ctgttgaagc gtgcgatctt ctctcgtcgt
    20101 tcattgagta gcctcgtgtg gacccttgcc gtccctcccc gaggggcggc atgctgttcc
    20161 catgaacccc ctaacctctc attgtttccc tatgcgtaat gttgaacgtc tcgtaaagct
    20221 cattcaagaa cgcaacgcgt ttgaccactc cctcgcccgt gaagtggcgg acgttgtgat
    20281 tggtgactgg atgcgccagg ccgaactctt ggatcatgaa gaaccgatgc cggctggcgg
    20341 caatggcggc aatggcggcc atcgaggggc aaccgtcttt gtaagggtgc ctttcacgac
    20401 agaatgcaca ctttcagccc catgccagaa tggctcgtgt cgacggcatg ggaatccccc
    20461 acgcaacgcg tgctaagctc catgtccgtc taccttgaga acattgagcg taatcgggcg
    20521 gcatatcgtg ctgccctttc gcgtcgggca tctttactca cacgtaagca acctatgcaa
    20581 tcatttttgt ttgaggatct tgacgagcgc gggaagaagg cagccatctt tcggtactgg
    20641 tcggaagtcc cggcggattg ggatattgaa gtctttttca aggtagccaa gtggcggttt
    20701 accgagcacg gcgaacgcat cgcgtagtat ttgcatatct catttttgat atatggatat
    20761 ctccgctccc ctcctcatgc cacaccagga tgccggcgcc gcatggctcc tcgctcaagg
    20821 cgcgggtctg ttggcgttcg aggcaggtac aggcaagacc atctccgccc tcgaggccgt
    20881 gaagcgccgc gatgcgcgcc gcatcttgat cgttatgccc gcatcgctcc tcgaccagtg
    20941 gcactctgaa atcatccgct ggggctatgg cgaggacgtc tatcaaatga cgggcgggaa
    21001 ggcagaacgc gaggcggtct atcgtggact cgcccaccct ggcgcgctcg tcgacatggc
    21061 accgtctggt cgcgtgttca tcctcacaag ctacgaacgg tttcggttgg atgcaaaagc
    21121 ggcgtatggc gtggcatggg atgcggtcat ccttgacgag acgctcaaga tccagtcgcc
    21181 gacggcactc atgacgaagg cggtgctact catgccgcgc ctccccggcc agctccgcat
    21241 tgcattgaac ggcacgccgt acagcaatgg ctggggtgat atctgggcaa cggtctcgtg
    21301 gctcgcgccg ggcacgctgt accggacgtg gtggcagttc cgtacgatgc atgcgatcgt
    21361 gaatccatac ttccacggca tcacggggtg gcgaggggtc gatgagatca aatcgaaaat
    21421 caaaccgcac atcatgaccg tacggaagac ggatgtttta tctgacttgc cggagaaaac
    21481 agagatccaa attccggtaa ccctttcaaa aaaagagttt gcggtttatc accgcatcaa
    21541 agaagaactc atcctagaat gggacggtga ggatacatcg ctccccaaca tcatcgtcaa
    21601 aatgatgcgg ttacgccagt gtgcgaacgg cctgttcgcg ttccctggcc atggcgaggt
    21661 gtcgtcgaag ctcgatgtgc ttggtgatgt gctcgaaccg ctcttccaag cttcactccc
    21721 cgtcgttgtg tttagtgagt ttgaagaaac agccaaccgt gttgcgcagc agtacggtct
    21781 ccctgtcatc tcggggcgcg tatcacagaa agaccgtgct caaattgtgg aagacttcaa
    21841 cgctggaaag gtcaaaggca tcgttggcac tcgcgccatg gcgttgggat tgaacttgca
    21901 ggcggcatcg cacttggtga acgttgacct gccgttttcg tatgcgcaat atgaccagcg
    21961 tattggccgc gtctggcggc atggccagaa gaatgcagtc acggtctata atctcatggc
    22021 aaataacacc gttgacttca atgtctgggc gattcttgcg cgcaaactct ctggtgccga
    22081 cgagctcgcc tcagtcacgc gcgcagacgt tgacgagatt ttgtccacat aatgaccctt
    22141 gacttttctt ctaactcgag ttagaataag atcgtattac agcgactaaa ccttactcac
    22201 ttttattcgc aggttcctat gtccgaagaa ctcaaaggca agcttgaaga agcgatcggt
    22261 ggtggtcctg aaccgaagca ggttgacctt ggtctcttgc ttgaagccat gatcgagaaa
    22321 ggcggcggcc agatcgtgct cgcgaaaaat gggaacctat tcgccgtctg tgtcgtctct
    22381 ctcccgttca cgggtgagct atcgaaacaa accgagccgg cgcctgccgt ttgaggtatg
    22441 caaaaccgct tccaaaacaa gaagcgtaac tgctacacct gcggggagac gatgaagaac
    22501 ccaaagagct tgacaaactg cgtaaaatgc cgggcgcgaa acaaagaact tcggaacgta
    22561 aacaacgcgt acattaaaag gtcatccatg tgggtgacga tggagcggcg gggattggtt
    22621 ggttccccgt gatccctcag cggatcactc ccgccttccg ggatcgatcg agcttagacg
    22681 actgtgtttt ttagagccat agtcgtcttc ctagaaaggg cttgatcgag actggaaggc
    22741 gggaaaggta aagggttccc gcgtctgtct cccatgaaag ggggcgcgat ggtctggatc
    22801 agggcgcaag atacgtcctc ccagtgcaaa gaagaaaagc gccatcgcct tcccttcctg
    22861 gtctgatgag agcgtgttga ttgccgttga tacgctcaca ccccatcggg ccaggaaggg
    22921 gagtaccctg ggtacgccgt cccaccaaaa cggctctcgc taagagtggt gacgctcgac
    22981 gacgctctgt tcaaggctct gtgtgctaat cctgttcaaa ggtacatgta agcctttact
    23041 tgtagaaccc acatcccttg catcgtaagc cgcatcatca ctcctcttag cgggaacatc
    23101 gcaacgtacc tggcatcaaa ctaatagtgg actggagcgg atgcctgaag tccacggccg
    23161 ttgccttcct gctggtctgt cccgaccccg taggcactgg gaagccctag ggtttccctc
    23221 tcgccgcgag tggtgattaa gaggcttggc atagcggcta ggctaagtcg tgagggaaac
    23281 gtcacgctct acaatcatca ctcacggcgg gaaacgtacc cgcaggaaaa acgaaacgat
    23341 ggtatacgga gatagcccct acggccatcg ttctcgccgc gagtggtgat gtggtttcaa
    23401 accagtgatc ctggtgcgca tcatcactca cggcgggaag cagcagttcg ggtaaggtgg
    23461 cctgtatacc caccgaaacc gacgtaagcc agtatggggc tgttgcaccc gcactatctc
    23521 ggggtagtag taaggtctta ggcgggcccg ctgatggcca aaaaatacca gcccttaaag
    23581 ctggagatgc aggtccaaat cctgcccctg agtccatggc ttgacggttt accaggccgt
    23641 cgagccccgt tgagaggtgg gaggcatgta taatagagag aacctggatg acccttatac
    23701 ggctccttga tcttgaaccg cctacctctt ctcaacggca cttaagccga tttatttagg
    23761 aggcaattgt tatgaagaaa ctgcttgttg cagtctcatt gttcgcaaca gttctcggca
    23821 cggcgccagc cgcgctcgcc gcctcggctt cagagaacca gcttattcaa gcccgtatcg
    23881 atgcgatgct gtacgcggtg gaaaaccacc acccggaggt gtctctcgct gctgaccggt
    23941 acaacacgga cctcgcgttc gtgatgagtg ccaacggtat cagccgtgag gacgcgattg
    24001 cccgcatggc acgtgcgctc attgcacaga acggccgcat gccggctccg ccgatcccat
    24061 cgaatgtggg agcggcatat gacccggctc cccatgcgtt ccgccaggcg caagcccgga
    24121 cgttctacgc aggtgtggat ccagttaccg gcacgcatgt gttcaccgtt gaacagcgcg
    24181 cgtcgctcct cgactcgttc tggtactgca caccggggaa ggtatacacc tacaccccgt
    24241 cgtcctcatc gtgtaagtaa gcccactccc ccacttcttt gtgggggatc gggagtggag
    24301 ggtgtagctc agtttgcccc gcaaggcctc tgggactctc cccgccctcc actctcggtc
    24361 tataattcac tttcttttta tgacaccaga gaagaacctt aagcagattg cgatcacgaa
    24421 agtctcgaat ggcttcatca tcgacgagaa aaatggcgga acgtatgtcg cgtgctcatt
    24481 tgatgatctg aaagccatcc tcgatggctg gaattccgca gagtaaggct tttagtatgg
    24541 cttccccgaa taccaagaaa ctacaggagg cgcgccgcct cacacggttg agccgcgtca
    24601 agatcgtgcg tccagcagag ccaggaccta accgggcgga gcgtcgcaag aagatccggt
    24661 tgcgccccaa aatgaaggcg gaacgacgtc agctcttgct cgatatcaag aaccctccgc
    24721 gtgtgaaacc agtaaagaaa acagcaaagc ctgtacagaa acccacggcg aaacggaagt
    24781 tcttcaaccg gcgatcctcg tgatcgcccg tcgtgaaggg gggatatggg acagtgagcc
    24841 caacataaga aggacgtggc aagtcgcgct ctcattgact tctccttgag agcgcagcct
    24901 cgcaaaagtc tatagcctct tgcgagggag tagcctagga gtcaaacctc ctcccccctt
    24961 cacggcgcac ccttaacggg acagtgcgcc tgcacctttt attgtgagtt gctaatctcc
    25021 cttcattcgg acaggcgagg gatccgactt acctttcgcc cgttccgatg ggttcatgtc
    25081 tgccagagta aaacggacat gagcccttcg gaacgattaa taatttattt ttcttatgat
    25141 ggatattgca cgaaaaattc tcatgggtct cgtcttcgcc ctattcgctg ttgcgtcatt
    25201 tgccattctt ggcagccgta tctacggcgt cccattcaac tggtgcccaa cgaaaaacta
    25261 tttccttacg attacagctc cagctcaggg tgcccacgga aatttggttt actctggaca
    25321 gctttgcagt aagcagaatc cagtcgaagg gatcaacgct tccattcgga agatgctgaa
    25381 tctcggggag aaggatggtt tcgtgatcga cttcatgatg gaactatgag agaagaggac
    25441 ttcctatggt ttgagatcgg atgggagccg cgctacagac gacggtttca cattcaagag
    25501 gctctcaaat tcctctttac cggacgcctc acgattcggc tccgtcccca tgaggcgaag
    25561 caggtagcct gggctattca aacaaaacaa cctctcaatg agaactagca ccatcattgc
    25621 catcatcaag acggcctacc tcgccgcgat cggcatcgga ctcaacctct actatcccga
    25681 cttcggggtg ctccgtacct taggcgtctt cactatcttt ctcgggttcg ggatgatcca
    25741 tcatgcactt gaaaatcaaa ttgaatacga acacttccat gccaacgatc gataaccggg
    25801 atcctatccc gaaatgccaa gaacaaggtt gcacacgaag ttgtaagtcc atgggccgta
    25861 cggcatcagg acgtcgacgc ttcaagagcc gctgcaaaac acacctagat ggccagcaag
    25921 atccgcctaa ggcagcacgt gaacgaagcc agaagaaaaa aaagatgggc cctatcgctc
    25981 gtgaggtgga ggcgatgagt cgtcgtgggg tacgtgcgtg gctcaagagc ctgtgatcga
    26041 cttcatcctg gcgctgcctc tcgttctctg gcgcggcgtc tcgtacggtc acgtacaagg
    26101 gagggcgctt ctcgcgcgtc tcgcagtcac gctctccctc ctcactcccc gcccgattct
    26161 tccacctgca ctcatctcat tattcatcga taccctcgcg acgagggatc tgcgtggcct
    26221 ccttggctct ccacaccgga tggatggcct ggtcgacaag ggcgcgcagt atctcatgat
    26281 gggcctcggc gcacgtacga gcgagcagag gatcgtcgtc ctcaacaccc tcattgccta
    26341 cctcttgctc ttcgcgaaca tcaaagattt ccaatgggga ccgaagcacc gggctgaagc
    26401 taccctcggt aacccgctct actatggatc atactcatcc atatcatccc tactcgcact
    26461 cggactccac ggctggggca agctcctcgc agggcctctt gctctcggaa cgatcctgtc
    26521 tggctctcgt gctccgctcg cagccaccgc agctggtctc ctcacttatg ccgcttctga
    26581 aagacgagca agaaagccca tccttggagc tcttgccctt gtggccggag gtgccgttgc
    26641 cttgaagttc ttgcaacgac gtgacctgaa tcccaagaag actgtgatct cgcgcttcat
    26701 cgtgacaggg gatttcaacg cccgtcatgc gttctggaag gcggcgatca aggcatggcg
    26761 cgcgaagccg ctcttgggtt gggggctcgg gagctacgag aacttgttcc atgcctacgg
    26821 tgacccacaa attatctatc gttcacacta tgaaacctgg ccagacaaag cgcataacac
    26881 gtatctcgac aagctggcag agtcaggggt tgtcgggctt ggcgcgcacc tactcgttcg
    26941 agctcttgcc atctggcgag ctacgcctca cgcccgcgcc gccctcgtgg cgtcaatggt
    27001 tcaagatgct tttttcttcc ctcatccgtc gaccgcacac ctcgacgcag tccttactgg
    27061 acgaagtctt ggacgcgcag gaagaaaacg atcgagcctt ccgggactca ttggatctct
    27121 tccacttgca atccttactc tcttcggcgc gcgaagaatc aaggatgaac aacgagctgc
    27181 ccgcgccgtg tctctgttgc aacaaggtcg acatgctgaa gccaacgaat cccttcgacg
    27241 aatccgatct ggaattgacg ttcgaccgct cctcggaatg cgactgggaa ctgaaacact
    27301 ggagcttctc gacgccatcc tggaccagac ggacgcggag aatcccaagg acgccaagcg
    27361 acgatacatc catgccggat tcctcgactt gaacaacttc aagaaagaga aggctgggga
    27421 aatctataag tgcgcgctcg agctcgcgcc tacgaaaatc cccatcctcc tggactatgg
    27481 ttcatacctc gactcaatcg gggacgtggt cggagccgag gctcagttcg cctatgcgaa
    27541 gaccatcgca cccgagcttg gcgagaccct ctggcgctac gggttgcacc ttgcgaccta
    27601 cgggaagcgc gagaaatcag gggcagagat gctcgttcag gccgtcgaga acgtgaagta
    27661 taaatacaag cctgtgtcaa gtggggatat ccatatttac gaaacagcga aatcattact
    27721 atgagccctt cagaaacagt aaagcagcgt gagtactaca tgagccttgg acgtgcgatc
    27781 tttaattcag tcaaggggta cggggctctc gttgacgaca gcatccgtca tgcggaaaag
    27841 tgggaaaagc tatccgctga ggcgcagcag gtattcggtg cggctgccat tatcgtcacg
    27901 ggtttgcatg agtttgcaaa aggagaatcc gctctcgtgc caaacgtttc aatcacaaag
    27961 agaagctagt cctatgaaac ccctcatcat cctctcactc ttcgtcatgt tgtgtggggc
    28021 tggatgtaaa aaagcaccag aagtaagagc tactctccac cagtgtaccc ctttagagct
    28081 gcaaacagat gagctctgca tacgtctgag aaaagaagtc gaggcagaca atctccgcga
    28141 ccttgaagct aagaaacaaa aacccatcat ggtcacggtc aagctcaaga cgccgatcaa
    28201 aacctggtgt ccagcatacg atgcgccaaa cggttgcacg gaagaataca aaactgaaac
    28261 cactacccaa atccctcttc aagagttcgt cgatctcctc gacttcaagt acgtccccgc
    28321 aacctcaacc acgtctaacc aaccagccaa gcttgtaaag tgaatatgaa accaggaacc
    28381 ttgatcatgc tcccagatgg aagaattggg acagtcgttt accacggact tgatgggtat
    28441 ggcatccagt ggggggaagt aattgtggat gaagctattc tgaaatccgt gaatcctctt
    28501 tttggcgaag aaatagaaga ctatccctat gtaccacaag cgatgcttcg cgacccctat
    28561 aaaaatgcgg atgttgaatg tgttggtagt gactacaaca tcgttacccc cctaaccaac
    28621 cagcaaagct cgtgaagtga atatgagacc aatcaagttc cgcgtctttg atacgcacca
    28681 taagtgtatc cgacaggctc atcaaatcga cgttcgtatt tctggagaga atgtatttgt
    28741 caatacgggt tttggtgaac ttaccgaacc atatattatc ctcatgcaat tcactggcct
    28801 cctcgacaag aatggcaagg agatttatga gggagacatt gtgaaacaga catggcaaac
    28861 tgtggtgggt gttcttgcac caaacgaaga tgaggtttat gaaacggaag agtgcattac
    28921 ggaagtcgtt ttcgagaatg gagcattctg cctgaaaaac tcttatcaca attacggtgc
    28981 cattccagta tttgaagcag tcgataaata cacggagtca tttgtcgaag tcatcggcaa
    29041 catctgggaa aatccagaac tcctcaccca ctaaataaat gccagcgatt gcctggtgaa
    29101 gtgaaacgaa tatgatcaaa aaagaacccg taaaaaagac attcggcgac atcctgcggg
    29161 agcacggagc ggtcttttca atctgccttt ctgacggcgg atacgccaac gtcgatctca
    29221 accacgacgg aaagaagtgg gtcgtgacat tcctgaacga cgacggctgc aaccgggaag
    29281 acgagatccc aatggaagac gtcgagaagg tggcgcgctt cgtccagcag ctgcgcgaca
    29341 tgcggttgcc cgatttcaaa actgacctgg tgaagtaagg atatgaagat cgaactaaca
    29401 aaagaagagt gctgggtgct cggagagatc gtgaatcacg ccagttcatg tgttgatcga
    29461 gatacagttt ccgtttcctt tcgaccagac ttgttgactg aaaaacaatg gagagacctg
    29521 gcctacaagc tctatcagcc gtatggcctc ggctctccca agaaatccta atatgccccc
    29581 aacaccattc ccagcaggga agacagtacg tgaattgaac attgatctta aaaagaagtt
    29641 tatagtggtg aaaccagacg gcgaaagcag atatcacggt gtcaaggaag gagatattgc
    29701 ggttgcaact aaacactggc aaacagatag tgattgcatt acttgcaaaa ttgaacgtcc
    29761 aggaggtcaa atgatatgca cgtttcaact aatagaactc gcctactacg aacccaccga
    29821 acaggaggtg tgggagccga aggaaaagga tcttgtgaat ctcaaaggcg ctgttgttca
    29881 agtcaagcca aatggtagtc tcactgtttc agtgggtggc tacaacctcg cgttccttcc
    29941 aaaagacgtt gaattcttct cccgtccagc tcctcgtaag gtgacgatgg ccgaggtttg
    30001 tgagcggttt ggggagacag tgattattga ggggtgatat ggcttcttgg gaatataagt
    30061 ctgtctacac aacgtacgat tttggagctg gtttggagtt ccttctcaac tacgaggcaa
    30121 aggatggctg gatattctcc agcttgaaga gtgaaggaga agaaaactta ctaacattga
    30181 aacgaaagaa gctatgacaa tcctctcctt ggaaacaagc aaaaaggtgt atgagctgtg
    30241 cggggaatat gacacagaga gagtgttcgc ttacgatttc gatggaagat ataccgtgat
    30301 gtttcgtgaa gagctggaga tttgtttggg cgatgttccc gctcccaact tcgccgaact
    30361 catccgcctc ttgcggaaga taggggagaa gaagggatgg aaacaggggg agttctctga
    30421 tgctgttgga gtcgcttttt ccctactcat gctttatatg aatgccccaa ccgaaccaga
    30481 aggcatgaga gctgtcgaag aatacttgat gaaactcttt tatgaccccc gtcgataaaa
    30541 atgccattgt agacatgctg gtgaggtgcg tgaataaatc cctcaaggag ttcgtcccaa
    30601 ctagtggaag attgagcgaa ccaatgatat tcatcgacgc tgcgcgtttc ctccaagccc
    30661 tggaagcgga gagggaggag attgtaacag atgaaccgga cggtatagcg ctaagccgct
    30721 atgaagccct caaaagactt tctcaatatc cggactcgat tcgataaaga gcggaaaaag
    30781 aagtggcttg cggcacgtag acgggaatat ctgagatcgc ggaaaggcgg gtatctcgtg
    30841 aatacgttcg ctgcgcagcg gagtatctgt tactactgca agcggcaagt cgtcttcagt
    30901 gaatggagca tcgaccacat cattcctatc gtgaagggtg gtaagacgac ggaagagaac
    30961 tgcattggta cgtgccggac ctgtaacaac ctgaagggaa gcctgcccca cgattacttc
    31021 aaggcgaaga tggaatccca attatcacta tatgaatccc aagatcttct ttctcgcgat
    31081 gattctcctc ctgatcgggg tcacattgac gatgctgacg cgtgattcag cgagcctgct
    31141 cgtgttttac acccgatctg ggttcctctt tttcagtctc gtcctcaatt cggcaaacat
    31201 cctcttaggg ctcaagcaca aatatggatc tcaaaaaaac tctcaaacaa cttggactcg
    31261 atgaccagct tccgacggcg atccttgttg tgacgacaat cgcattcgct gcggccgcgg
    31321 tctactggta caatagtcgg catgcgggaa agcagggacc taattcccaa gctgaagaaa
    31381 caccttaagg aaacctacgg ggcctcctgc cacaaatacc atggaggccc cttcggggaa
    31441 gcaggacacg ctgatctctt cgggaccctt ccaggcggac gtgcgttcta cttcgaggcg
    31501 aaggtccccg gcaatacccc tacccaagca cagctcctct tcctcgaaaa agaggcagcg
    31561 cttggtgccg tgacgggggt tgtcacatct gtacaagatg tggataaata cctcttgccc
    31621 ttgacaaaat aactcttctc atctaagtta gatgagaaat atggcggaac tcctgaacac
    31681 gcggaccgta caagttaagg tctacgtttg ccagcgctgc acctacgagt ggcagccgcg
    31741 ccatttgggg cacgtccccg tgaagtgccc acgctgccgc aatgcgaatt gggataagcc
    31801 ctatcgtcgt ttaggtcttg cgaagtggcc tgaccgaatc aaggaaagca aatgatcaaa
    31861 gagggatatc gtctcccaaa gccgacaggc gaggagttcg accctgagac gaatgcgctc
    31921 cgggcggaat gtcttcggct cgggctctcg cctaccctct ggccaaaacg gatcccttca
    31981 gataacctgc cccccgagga aatccgtgtg gaagaactcc gggcatcatt ggccgagcat
    32041 ggccgcatgg cacgcgcccg cgcaccaaag tccacagaac ggattgggaa ggtgttgcag
    32101 ctttttgatg ctcacccaga taggaagaca ttcaccatgt gcgtcaacat gctgagtgaa
    32161 attcggtttg atgaatggat cagtggatga tcccatcgcg tccgcctttg ccgaacccac
    32221 aggtggacgt gtaggttcat tcattttagt cttattatga tcaatccaga ccacaaactc
    32281 ccggaaccgg aacgacgcgt atacgagccc atgccggaca acgtgtacca agcgcagctt
    32341 atcgacgttg acctcaagca gaatcgcaag ttcaaatccg atgaggagga aacccgtctg
    32401 caatttacct tcgtgatcat tgacgaaggt ccctactacg gccgccgcgt atgggaagac
    32461 gctcgccaga tcgtctccat gcggccaaaa acctccaagc tctacgagct catcaagtcg
    32521 cttacgggca agacattcac cgaggaagag tgcaagcgct acacggaaat cgtgacgcct
    32581 gaattcctga acggtatgat cggaacgcag aagcgcctgg cgctctcaac ccacgtcagc
    32641 caaaccggca acaagtccaa caagatccag gccatcatgc ctgttgagaa ggaacttccg
    32701 ccgtacgacg agaccaaggt caagaacgag cagtagattc ttggtgcctg ggttcggcag
    32761 cgcacatata aatacccgcc atctggcggg tatttatatg ttgcggtgat ccggcagcct
    32821 cacctattgg ctcgacaatc tccgaagctc ctgttcaacc cctttattca gtcggccttg
    32881 cttcctgagc ccatccaaga acgtgttctt ttctgaggcc gtcttcatct tgcttgtcgc
    32941 ctcgaggata tagcgggctg caagctggtt cgagacgtcc tccgtcatga tcttcttctc
    33001 ctcaaggtct tggaggtaag cattcttttc ctcatcggag cccatcttct ggagctcggc
    33061 ccagatatgc gcggcgcgga acccattctc gatattgagc gtgcggaggt tctcctcgtc
    33121 gatcgtgtag ttgcggacag tcgacttgtg gatcgtcgcc atatcttcgt agagacgggg
    33181 gtcttctgcc ttgatccggt cgagctcggc gtttgcctgg tccttcggga gtttgagcag
    33241 gcgctggtat tcgacacgcg ccttttcctg ttgagcagag gcttctttgc gttgctcctt
    33301 ccgctctcct gcgtatttga cgttcgcctg tacacgctcg ttgtagcgaa cctcgccggt
    33361 ctggtttcct gtggagactt tgaccggtga gaaggcattc aagatgttgt ttgacttcat
    33421 aatcggcgct cccttggagt ctgtacgggc cgggacgttc ttcgaaagcc ctgggatctg
    33481 cgacatgatc cactgcatct gctggtcaag gagcgagcca tctgtatcag ccgcgcgctg
    33541 cacagggtcg atgatgttgt tgacccatcc catgagggcg cgggcaggga tgagctggga
    33601 ggcgtagttt gcgatgatct tacccccggc ttcagcatcc cctttcgcag cgctcatgag
    33661 gtcaccgatc tgtttcatgt acgactgatc tgcgaagaac ccaccccact tcgcaacacc
    33721 agaaagcgtc ttatcaagca tggtgtcatc cacttccccc cgcttatttg catcgtggag
    33781 cgcagcaaag agtgcaaggt taaaggcaag agctggatgc agcttcgagt aggagtacca
    33841 cttatccccg atcttgacgg aatatggttg catgccagct gcgaggaagt cttccttttc
    33901 cttcttgtcc gtcggcgtac cccatgtgat ccggtcctgg cctaagagcg atgcagctgc
    33961 tgtggtcgtc ccaataccca tgaggatctt agcgatcttc tcggatttat tggctgcgcc
    34021 agggaggttc acgataccaa gcggggagta ttcgatcccc tgtttcaaga tgttcactgg
    34081 cgtacggacg aacggcaggg tgaacttcgc gatcgttgag acaactgggt tctgcgaggc
    34141 gcgtgcctgc atgacgacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
    34261 cgccttctcg atcggcgcga caagcgccgt accctggaag ttcgatgcca cgttctgaat
    34321 gtgcgtaagg ggtgatgaga gcattgaatt gtagcggagc gtatcaatcc attcacctac
    34381 attaggcttc acgtgggcac ggtaaaattc cgtgattgcg ttcgcgtcat tccagtccac
    34441 gttcttcgcc tcgtcaatga ccttctggaa atccttctcg cgcttcgtga cggcatcgat
    34501 caccgtgtcg atcttcgaca gctggtctgg tttcgccatg atcttccgcg cctggagctg
    34561 gcgagccgta tcagctgacg ctgcccggtc acgtacgagg agttcaatga aatcgggagt
    34621 aatcttccca ttttctgcga ccttcgcgat ctccctacgg aggttcaggt tctcagctgc
    34681 acgctggagc gtgacatcac gccccacggt ttcctccata atcttaccgg tatcacgggc
    34741 atactgttcg acttctttgt tcgtgagctt ttttccaaca acctcttcga tacgtggctt
    34801 ggcaacagac acgccttcct cgactgactt ctttgcaccc tcagagacat cgagtctagt
    34861 ctgcttgaca ttttctccag aaggtgttac accttcgcta tatgacgaca attctctcgg
    34921 tgcttcactc ggcactccac ctggcgtgtt cccttctggg atacgcaatt gcgattctgt
    34981 tttttggatc gatggtgtgg gggctggctc acctttttta aaggtctcaa gtagaccctt
    35041 ctcttcgttg accggcggga gttctttcgg gaacaggttc ttcggagcaa ggcgttcaac
    35101 ggcaacagcc tcctgggctg gttttgacgt tgcgagtttc ttgatagagt tcactgtttc
    35161 ctcgattgca ggagccgctt tgtcgactcc cttcgcgatc acctcaatac cttcctgacc
    35221 caccttcttc gccgcaccct ccccttcagg catggcgacg tcagccgcct taaggccacc
    35281 catcatgaga gcgccaagac caatgcctga aatgcggctg agtttggcca gagggctcga
    35341 tccaggcgtg atcttgtttt gcgttgctgc ggccttctgg cctgccttat aggcctcctg
    35401 cgggtcaata ggttcattgc cgagcacgag cttctggagg cccgtcttgg gcgccacagg
    35461 ggctttaacc cccgcaatac cgagagccgt ctgcgcaacc gtacggggag ccgcctggag
    35521 aatcgtctgc ccaaactgga gacccttctt gagtggggct tcaacgatat caattccttt
    35581 ctggaccgcg cctgggggct ggactggctt attcactatg cgcgagaaga gccccggctg
    35641 gggagccgga gcaggtgtta caggtggagt tgccccagac gttttcagaa agaggttgac
    35701 gagtgggcta ggcatagata tttagaaacg gaatccgcca ctgcttgcag atcccttggc
    35761 tgaagcgagg gcacctgcgc cttcgagacc gagagaagaa acgtccgttg cagacatgcc
    35821 ggttgaagcc atgacggacg cccggttcgc gagctccgct ttcgacggct cgatcccttg
    35881 ctggaggaaa gactggtagg cttctgcgat ttggccgtca ttcgcaacgc ctcccccgcc
    35941 ggtaccagca cgggcgcgtt tagcgatctc actcttgata tcgtcgatct tgtacggaag
    36001 gagatcgaga atctccttcg tccgtgcagc catatagccc gtattggctt ccgcatttgc
    36061 cgtctttgcg ccagctgtgt agtccgcttc cgcgctcgct ttcccggcct gcgcttcctt
    36121 gagggcgatg ttcgccggcg actccgcttc cttgaggagc atgtcacgtt cttcaagaga
    36181 gatcttccct tccttgtagg ccttgtcgat atcattcttg tcgctcgcag cctggctctt
    36241 gaggtatgcc gcatcagctg tggtctgctt agccttcgag tcgataacct gtcggatcgc
    36301 gtccgcgatg tttttcacgc gggcttgcgc atcggcctgc atttgcgcac ggacactcgc
    36361 cgccttgttc gagaatgccg ccgaggcttc aagtgctgct gcgtcacgca tggactgtgc
    36421 ctgctgatcg aagcgctgaa gctccgccat tgcctgggag tccgtggttg gcgtcacgcc
    36481 gcgcgctgcc atcgcatcga tgagcgactg gcgctggata cgcagcttgc ggtccgtctc
    36541 ttcgattgca gatgccttcg cttgctcagc catcttgagg agctgttgtt cctgagcctg
    36601 tgcgttctgg ctgtttgcat actggaacgc ggaagagttt gcaatcttcg attcctcctg
    36661 gagctttgcg taattgtagg tatccattgc ctgctggaag tccgcatcag atgcaaatga
    36721 gctccgctct ggaaccggga tattcgaaga cgtgacctcg atgccgcggc cattgaatac
    36781 ggggttcgta tagtcgaccg cttctggttt gtaggaacta agagctgttg cgacgctctt
    36841 cgcgtaatcc gaagggcttg taacggcgga agcgcctggt gggacagatg cacccggagg
    36901 ggtatacggg acgtatgacg gggaaggcgc gccacctgcc gtcggggatc cgcctgcatt
    36961 tcgaagcgcc atcgcgacat tcccggcgaa ggcatcctgg gagatgtttg gatcgacagg
    37021 tgatccgaca agacctgcgc gatcctggag ttgggcctgc gtcgacatct gcccggcatt
    37081 cagctggaca ggggacgggg ctgctggtgc cgcagcagga gcgcttggga cggctggagc
    37141 agcgacagga cgcggaacag agaccgacgg ggtcgcgact ttccggatag ccgaagccgt
    37201 cgcggtgggc gacggggggc cgtatggggt agaccaggga tccttcttca tcatttggtc
    37261 tgctgtaagt gtcttcggtg gaagtacgga gaaaccccca tatccgccgt tgtccttcac
    37321 tgcggtcgca gcaggaccgt atgttgctct gatcttagag aggtcaatag ccatatggat
    37381 tatgctgaga caatttgagt tacgacaccc gcgctccccg aacctccagt tgaaccggtg
    37441 acgccggtac cgatcccggc accccctgcg ccgggcgtac ccgcgatcgc ttggatcgtt
    37501 ccagagttcg tgatcgtgcg tcgcacaata atgagccaac cgcccgaacc tgctccacca
    37561 ccgccgccgc cgccaacgtt tgaacccgta cccgtagctc ctgcgccacc attaccgcca
    37621 ttacaacgaa tggtccctgt atttgtaatg tcaggtgcag caacgaaacc aaagccgcca
    37681 gaggagccac cgccgccgcc agccggctcc tggacgctgg atgcggaacc accacctcca
    37741 ccaccgccgc ctgcacccgg agcggtattt agagcaatga tcgctgtgcc atttacatca
    37801 aaaagcatgt acgcacctgg aagactccgt gggatattga gcgctgcacc cgtattcgtt
    37861 cccgaggcgc cgcctgtccc accagcgttc gtgccagaag taccaccatt tccaccggca
    37921 ccggacgtcg tcgcagaaac agaatggttc gcgtttgaac cagctgaacc attgtttcct
    37981 ggagcattgt ttacgccacc agaggcaccg tttccaccgg cgatgttcgc cggcatggaa
    38041 cctaccgctg tcgcagcacc accggttccc tgggtttcac cggatgcagc cgtaccggcg
    38101 ttcccatcat tcgacattgt cccctcgttc aaaatcgacg tgttcgcgaa cacgtggaag
    38161 ttcgccatct tgagcgtaga accaacacgg acgcggagcg tcgttgcgaa gacgtcgcgc
    38221 gtcaacgtat acgtggagcc tgatttcgac gcaaaggagg catacgtatt cgtaccgtcc
    38281 atgtcgaccg gaccatcgga accatcacca taattattcg aaacaaggcg agaatccaca
    38341 tagtctttcc ggaccgcatc ttcagccgca gtcggcgtcg agaccttcgc ggaagtcaag
    38401 cggaagtctt ggatcgcggc ccaggtattc gctgtatcaa gacgcgcaac gattgcaggg
    38461 tccacatcca tctggacaga acctacgcct tggttgttac gaagtccgcc gcttgatcgg
    38521 aggttcgtcg agatgacaga agtgatgatc tggagaccgt tccccgcaga gaggcctgat
    38581 ccaccggaag caatgtcgta cgtattcaca ccatcgttgc tgataagcca cttgttcgtt
    38641 gcagcgtcgt accgaacgaa cggcttattg ccgtcggcat tctgcgcgta aatcgtgatg
    38701 gtcgaatcag tgtcgtcacc aatgcggaga acgagagacc ccgtcccgcc tacacccgtc
    38761 atgactttta caacctcatt ccagtggcga tggaccggac caatgatgaa atccacattg
    38821 gagctatgtc cacgacggaa tgaagatccc gcatcagtat ctgcatccgc ttcaggatca
    38881 agaccacgaa ccacggtgaa tgtgttcccg accggagccg ttgggatata cacaagctcg
    38941 gctccagaag agccgttctc aatcacgccc cacgtcggat attcctgcgc gccaccagga
    39001 acatcattca caacgaggct tgtcccgcta tctgaaatac cctgagcgag ggtcgtcttg
    39061 aagaaggaaa cggtcttttt gaaaatgcca ggcataggat taaatttggt tatcggcagg
    39121 gatacggtca tccgcatcca tgacgtagtt tggcttgatg taggtaatga cgaaatcaac
    39181 gtcgctctgg taattctcga gcataatacg gagctcatcg aacttcgcat ccgagcacat
    39241 gaccgctacc caaaatggcc ggagctcgat gttcgatgag taacctgcga accctgccca
    39301 cggcacacca ccccactggt cagaacccca tgtcaatgag tagctcttgt caacatatgg
    39361 cccatcacca ctaatggttt tctgtgagag gggggagttg tacttcccat ccccgatgag
    39421 cgagacgccg atctcgcacc cagaagaaat gtaaccagcg atcttgacat agcgcccatg
    39481 cttcgaacgg atcgggtctc caaagttgaa tccctttgta tcgaagcgcg catagatcgg
    39541 aaccccatcg tcattgtacg tcgatgagtc gtactgatag acgttcggat ctgtcgcaga
    39601 agcgaagcag aggttccccc cataaaccgc gaactgtgta atggaatgac caatataccg
    39661 acgccacgat ttgtactcgt cgctatatac gagctcagca ttattcgtga gcgccccctg
    39721 ctccttgagc gcgaggatgt aatcgcgacg cgtataaatc gcggaggcaa gcgagagatc
    39781 tgactggaga agataggggg agactgggct cgatcgctcg gaagcgcgga ggtccgcaaa
    39841 cgactcctca tatccaactg cacggatgtc cttgaataca tcgataacga agttgtaatt
    39901 ctcgatctgc ttggtcgagc ggtgtgtgac agcgccgcag ttgatacgta ctttcttctt
    39961 gtagatcccg cctgagtcaa aaccgacatc aaagatgtcc ccatttgcga ggaatacttg
    40021 gacggattct gctccatcgc gagtgaagaa gcagcggacg gaaacgatct tcgcctggaa
    40081 gtccgtgctc ccagagccac cgcccgagaa gttcacgtag tcgtccgtga cggagtagta
    40141 gagccggttc gggtttgccg tgacacctgc gacgaagagc ttgagatacc caacctccat
    40201 gatgttgccc tttggattcg aaccgtaaga tgttacagac gatccatcgt aggacttcat
    40261 tgcttcgacg gcattcccat agatcacgat gttgtttgct gtcacgaaat caaacaactg
    40321 gcttgccgtg aagcctgtgt caaagctggt ccacactcct gcggcgagtt tctcgaggat
    40381 cgtgtcacgg gaccgaatca agatttccgt tccgtcatac ttcttgtacg tataaagcga
    40441 tgggatggag cccatgcccg tattgcggtc accaacgatc ttcacgccac gtcgcttcgt
    40501 gagcgcgaag cgggctgcgt aatccatgtt gacgatgtat ggagactcat tctcaagaag
    40561 ctgcgccgca ttcaaagccc agcggaggtt ccatcctccg ctaaaatcct gttgaacgac
    40621 gggcttatat gcttccatag ttaccaagct ccctgcatgt cacggacagg ggcatcgacc
    40681 tggatcgttt ccatgagctg catctctgcc agtgcgtcgt tgaatgcctt ttcggaatct
    40741 gtctgatctg cacggttcga acggtccttc cgaaaggaga gcttattgag gccttcgatc
    40801 acggtttcca tgaagcgctc cggaatcagg aagtaatcat tgtcactcgt tgggagtgcc
    40861 tttgtttcat tcgtcgtccc gtcaacacaa aggtctgagg tacaccagat gatgcggagg
    40921 ctccctgaaa ggggggactg cgtcggggaa aaaacacgga ggtacc
//
LOCUS       DAOQEW010000011        40582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_186_length_40582_cov_8.639302, whole genome shotgun sequence.
ACCESSION   DAOQEW010000011 DAOQEW010000000
VERSION     DAOQEW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 40582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40582
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_40582_cov_8.639302"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLS35_02450"
     CDS             <1..950
                     /locus_tag="PLS35_02450"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17540.1"
                     /translation="YTRLHRQLQEVTDRFFPETEWTSIDEFYADTTRLQTRHPDPRAL
                     GDALKADILHTTGLTCTIALASGKTAAKIAADAHKPDGLAVIEPGAEAAFLAPLPIRS
                     LPGMGPKSTEAVERLGVRTIGDLLLPRFESALARLLGTRLASFQSLARGLDSDPVVAD
                     REAKSTSHETTFDEDTNDPAVLEPIIHGFLETLAHELRLEGLAAGSFTLKLKDSHFRI
                     TTRQRKFATPLNYDPDMWPEIRQALGDLLQPRLRYRLVGLGLSDLVPAPEPLFDRRQR
                     DAVAALDRLIERHGTGVVRLGALPQSEAGTRKRRPRTPD"
     gene            1040..1522
                     /gene="rfaE2"
                     /locus_tag="PLS35_02455"
     CDS             1040..1522
                     /gene="rfaE2"
                     /locus_tag="PLS35_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNI17541.1"
                     /translation="MPTKVTTRDELVSLLAERRQRQQRVVFTNGCFDLMHIGHVRYLQ
                     AARNLGDLLVVGVNSDGSVRALSKGPDRPIVPDVQRAEVLAALACVDYVVIFSEPDPG
                     ALIAALQPDILVKGGDWPLDRIIGRETVEARGGRVQTIPLVPGVSTTTLVQRIRSTTA
                     "
     gene            1530..5003
                     /gene="mfd"
                     /locus_tag="PLS35_02460"
     CDS             1530..5003
                     /gene="mfd"
                     /locus_tag="PLS35_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNI17542.1"
                     /translation="MPPVVPSHLTNWFAPAREALRTGSGKPCLMGLHGSTAGFGLALL
                     TLSAPAQPLADRSWLVVAKTDDEAERLYRDTLFFRTLCGQSGDDLALFPKWETLPYES
                     TVPHIDLVSRRMQTLNRLCTTARTVLFTSVPALTQRVLPALVFTESILQFQPNSDLER
                     EVLVSSLLRLGYRKGSVVEIPGEFSIRGGIVDIYSTAYPDPLRVEFLGDTIESIRFFD
                     PATQKSTDKIKQAWVLPARELIRSDDAPDALTPLAADAEWHAPSVYGAMDSLLDYFPQ
                     PPVLVLDQPGALKAHTAECWQTIEEGYLRHEDRTDPNPYPTPDQLYLTWDQLVTATQD
                     YATLALEPVTAPDASWEPVLTCPAQTPASVGLGQRGTAFSQTLEVLDRLREGGPVVLV
                     ARSQGQVGRLLALFGEHDRPAMEWKPAALSAGGAQKAPFSVLNGEVSSGFLSPDLRLV
                     VLTEEELFAKGARHKPQHKSKAATFLSSLEDLNIGDFVVHMQYGIAKYQGLRRLSVQD
                     FDSDFLVLEFAGTDKLYVPLDRLSQVQRYAGADAHVPRLDRLGGTSWAKTTARVKKDI
                     EEMAHELVDLYANRELVHRTSYGKDSMLYHEFEAAFEYEETPDQRKAIEDIAKDLAST
                     KPMDRLVCGDVGYGKTEVAMRAAFKAVEENRQVAVLVPTTLLAHQHYDNFAERFAPFP
                     TRVALLSRFQSPKDTKAIIKDTAAGLVDVLIGTHRLLQKDVQFRNLGLVIIDEEQWFG
                     VKHKERLKQLRTQVDVLTLTATPIPRTLQMTMASVRDLSIIDTPPSGRLAIRTQVLRF
                     SEKAIREAILRELGRGGQTYFVHNRVETMERMGAWLQELVPEARIVMAHGQMDSKPLE
                     AVMLKFFHREADILIASAIIQSGIDVPTANTIIVNRADTFGLAQLYQLRGRVGRGGEQ
                     AYAYFLVPDEGNLSDDAQKRLTAIQQFTELGSGFRIAAADLEIRGAGNLLGKQQSGHI
                     AAVGLDLYLQMVEQAVQRLRGQVVEEEPDPTLRLSVSAYIPEDYVVDSHQRLSLYKRL
                     SSCGQLGDLALMHGEIEDRYGPPPDPVERLFELMQIRLLAKQLRLSSVVEQTHAVVIT
                     FDPKAKVSETAVQALMDRYKKRLRFLSPLSFELQMPHDDWSLVFPELNATLQTLHVCG
                     TNNQEPRTGST"
     gene            5035..5931
                     /locus_tag="PLS35_02465"
     CDS             5035..5931
                     /locus_tag="PLS35_02465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNI17543.1"
                     /translation="MTSPQTPDISPWRARLTGTALALCALGSLAGCTEPPQEEPVIAM
                     INGRSITQAEFDIRWEELSQATRARYEKEGGKRRFLDELIMRELLMQEARKQGLDQSD
                     DIREKTLRYREQLILDELLKDRIKTKVEVSKEELDAYLEKHANQLLANPKVQVSVMLL
                     PNVYAAKDLKRQVEGGGNFTRFALRYSIDERSRAKGGDLGPYRKGLLEPELDALIPSL
                     HPGVLSDPIKTDKGYYLMKVSPLEPEILQADQATRERLRQELLAEKRRKRLDDVFAEL
                     RTGATIRMADAARYVTDEPGRP"
     gene            6084..7043
                     /locus_tag="PLS35_02470"
     CDS             6084..7043
                     /locus_tag="PLS35_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI17544.1"
                     /translation="MRPTSLQSLSRPALAGLCLYVWVGLFPAGPVDAAKLEDRIVAVV
                     NTDLIMLSELKRDLLPDQERLRKLYSGEDLERRLKTAEAMAVTKMIERKLQLQAAKNK
                     GVEVSDQEVTQAVQEMKKQGEALNQADPNTTRSVREQLTLMRVVDREVRGLIMVADSE
                     MKRYYEEHQDRFAYPEEYQLSQILIKPRTPDGLSVAQGRAEALLATLKQGEPFEDLAL
                     RFSDGADASRGGRLGLVRQGELIPALEQALTSVPVGQITGIVETAEGLHIVRVDDKKP
                     RQFRPFEQVKAEIQSLVFQQKTEDQYQIWMADLKNKAYIEIKF"
     gene            complement(7202..7906)
                     /gene="yihA"
                     /locus_tag="PLS35_02475"
     CDS             complement(7202..7906)
                     /gene="yihA"
                     /locus_tag="PLS35_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNI17545.1"
                     /translation="MAHLSSVAQVGAHPALLPDAIIMKLLSAEFIKSSVSPEQFPADH
                     LPEIAFVGRSNVGKSSLINSLLHRKKLAKVSKTPGKTRAVNFFTVRTSDPKLPLLSLV
                     DLPGYGYAKVSKTMRAQWGPMIEQYLEQRQSLGAVILLIESRVWMPQDVMTIQWVQSL
                     GCGLIIVLTKADKLRQSERKPTLTEVRVACGLPAEVPVLLYSAETHEGRDALWGEIRA
                     QFNGLQKHESGTRELR"
     gene            7936..8712
                     /gene="mtgA"
                     /locus_tag="PLS35_02480"
     CDS             7936..8712
                     /gene="mtgA"
                     /locus_tag="PLS35_02480"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNI17546.1"
                     /translation="MSKSTGSLFGRILFWMTVLIGLPAAALALYWLATLPDVSRLAKH
                     HPTETAVMEARRAQAKEQGHPLHIKFAWVPLSRISPALQRAVVAAEDASFFAHEGFDW
                     EGIKDAALYNLEVGEFKRGGSTITQQLAKNLYLSSERSLLRKAREALITRSLEHHLTK
                     KQILELYLNVAEWGQGVFGAEAAARHHFGKSAEDLTIEEAALLAAILPSPRRYDPIRH
                     TAYLTRRQRHIVRWLERGNGRKAASINRLPPESLEVVPVE"
     gene            complement(8744..9172)
                     /locus_tag="PLS35_02485"
     CDS             complement(8744..9172)
                     /locus_tag="PLS35_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI17547.1"
                     /translation="MPALFHLAFPVHDLAAAKQFYVEGLGCVLGRESPTAVTFGLAGH
                     QLVAHLSEDELDRQKGIYPRHFGLVLTREEEWQALADRAEAQHLTFYQQPRRRFPGTR
                     IEHRTFFLEDPSGNLLEFKYYVHESAIFGERDYTSVGQST"
     gene            complement(9213..10151)
                     /locus_tag="PLS35_02490"
     CDS             complement(9213..10151)
                     /locus_tag="PLS35_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HNI17548.1"
                     /translation="MKRLRPVLLVHGGAGRRAMTVAQAACVERTLAEGHRLLQAGVPA
                     LSVVEEAIRLLEDSGLFNAGRGSNRQLDGVRRMDASIMEGRDLRAGAVASIEGILHPV
                     TAARLVMEKTAHVLLVGKSASRFADYFGLERAPRSRRSLTAQPKQLRPAAGVSRTLQL
                     HQAIVKTGRLRARSLGKETVGAVALDVKGTVAAGASTGGVDVMLPGRVGDTPLIGCGV
                     YADNDAGAVSMTGLGESIIRVAVAKEIADGLATGLSPVAAANRVLRKVVERVQGAAGV
                     LVLSPDGRCTIRHSTPHMIAGIIGPDGRAIVRDRFA"
     gene            complement(10148..10714)
                     /locus_tag="PLS35_02495"
     CDS             complement(10148..10714)
                     /locus_tag="PLS35_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17549.1"
                     /translation="MGSPVSRSHPLRQLFGALTEKSFTEHLGWPDLNVTEYVSNLLVE
                     FAHTDQLYKIQNTQGRPLDSVVEMLFESEVLLEAQSFERERDVHRHIGDFTLFMTGLF
                     PEYLRRLKSVGRIYHKDFLVDYVKAGKRSYGLVAEFGHYDPEQDTPLFRKLSENFELC
                     VTGLGFVRSDLDRMQDPACRRVKDLLLN"
     gene            complement(10813..11283)
                     /gene="dtd"
                     /locus_tag="PLS35_02500"
     CDS             complement(10813..11283)
                     /gene="dtd"
                     /locus_tag="PLS35_02500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNI17550.1"
                     /translation="MKAVIQRVTQASVEVEGQVVGRIGLGLLVLLGVAKGDGEGDLSY
                     LVEKVQALRIFNDDQGKMNRSLRDVGGALLVVSQFTLLGDTAKGRRPGFDLAAPPEAA
                     RAWYDEAVQRLRHEGLKVETGVFGAHMQVELLNDGPVTFIVDSRRPGAHADLKS"
     gene            complement(11310..11810)
                     /locus_tag="PLS35_02505"
     CDS             complement(11310..11810)
                     /locus_tag="PLS35_02505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNI17551.1"
                     /translation="MTETRTFKTSWGWMTVTASAKGVTSIDLFPSVRTVRHDSHRDEA
                     AASIVKEAQAQLLSYLDGERREFALPIDWSAGTSFQRKVWKAIAGIPYGRVRSYQWVA
                     MRVGGKQYARAVGMALGANPVPIVVPCHRIIAHDGSLGGFSSGLPLKRRLLGLEGTLT
                     QLRNAR"
     gene            complement(11812..13440)
                     /gene="murJ"
                     /locus_tag="PLS35_02510"
     CDS             complement(11812..13440)
                     /gene="murJ"
                     /locus_tag="PLS35_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNI17552.1"
                     /translation="MSDPAATPEPSQPAVDETHSVVKAAGLIGIATFSSRILGFVRDM
                     VLARLFGATPAADAFFVAYRVPNLLRELFAEGSMSAAFIPVFTEYHTLKAKRDAWELA
                     SATFTTLLTIVTAVTLVGILAAPGIVWLLAPGFRESVDKLALTTLLTQLMFPYLIFIS
                     LAALAMGILNSLRDFAAPAFSPVFFNIFTIACMLFLSPWLSEPILAVAIGIVVGGVAQ
                     FAMQLPGLKRRGMLFGLRFNPGHPGVKRIGLLMVPSLLGLSVTQINITVSTILASYFS
                     GGPTYLFYGMRLIQFPLGIFGVALATAILPTLSAQAARGALDELRTTIGFGLRMIFFI
                     ILPAMAGLILLRYPIVHLVFEHGSFTKTDTLATATALLCYAVGLWAFAGVRIIVSAFY
                     SLQDTRTPAITAGIAVGANIGLSLWLMTILDAAGLALATALASMLNGSILVAVLHRRL
                     GRVDWGAVVRSAGRVLAASIPMAGICLWVASAPLWMETGQWIVKSVMLFGGIGFSVIA
                     YLGMHLMLGSDELDVVMGLVKRKLGRVARKFGVA"
     gene            complement(13498..14160)
                     /locus_tag="PLS35_02515"
     CDS             complement(13498..14160)
                     /locus_tag="PLS35_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNI17553.1"
                     /translation="MATGSKPALTEAERARVEAAVQAAEQHTSAEIVPLIVGRSGLYR
                     EAHHRAGLLSAVLALTVLVTIETAWLPWGWQASNAVWLVGITVLAYSVGVWVGTWPML
                     LRLFTSRDRMRHKVHMRAERAFAQQGLAQTRERTGLLLMVSLLERQVYILPDQALQGR
                     ISVDQWQDIVSVVVERMKVGDLAGGLCHGIEAAGVLLARACPSREGDNPNELPNAVIQ
                     DL"
     gene            complement(14147..15052)
                     /locus_tag="PLS35_02520"
     CDS             complement(14147..15052)
                     /locus_tag="PLS35_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNI17554.1"
                     /translation="MRLMGRAGWAVLAGWLWFTLVPSLPARALDVPPLSGRVVDLAHV
                     LPPATTAQLSETLQAHESKTSNQVAVLIVPSLEDEPLFDFSHRVATTWKLGAKGTDNG
                     VLLLIAIKERKIRLEVGYGLEGVLTDARSAQIIRNEIAPKFRAGDVPAGVTAGVQAVL
                     HTIEGTYRAPESPTAVHSDGEVIGNTIVAVIVGVVIGLFFSRAHRVLGPVAGGGLSFL
                     VAPWVIPALIAGAASVALVSLMSGLGQASGGGRRSRSGPFDDTWFSTRHGGWGSGGLG
                     DSFGGSGGFSGGGGDFGGGGASGDW"
     gene            complement(15083..15673)
                     /locus_tag="PLS35_02525"
     CDS             complement(15083..15673)
                     /locus_tag="PLS35_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNI17555.1"
                     /translation="MARLMGASFLLAGLLALSGCGYNDLQGLDEDTKATWSEVINQYQ
                     RRADLIPNLVNTVKGYAAHEKDTLEGVVQARAKATSVQITPELLKDEAAFKRFQEAQQ
                     SLSSALGRLLALAENYPNLKADQGFRDLQSQLEGTENRITVARKRYIDKVAEFNKMVR
                     YFPTNLTAKFLLHLDEKANFTVADEKAVAKPPEVKF"
     gene            15847..16152
                     /locus_tag="PLS35_02530"
     CDS             15847..16152
                     /locus_tag="PLS35_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17556.1"
                     /translation="MALDRSTDTPGGFQVRHRALGIFQGSSIGLAFWHPSSHMPEYGL
                     CRFATRAKAQDYVDFLSSPACPEPLSQADLIIEDFDHAEHERLTTEYPQASAWETPL"
     assembly_gap    16161..16260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16313..16813)
                     /locus_tag="PLS35_02535"
     CDS             complement(16313..16813)
                     /locus_tag="PLS35_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17557.1"
                     /translation="MAQPLTCPQCGQDKVFRSPCSTLMERLQSLACMSPFHCQSCSFR
                     FSASRFGYRYPARMIDRREHLRIPVRLFLSFSGGKVRGEGTVRDISMGGCIIESQASV
                     HEDDIFYLQIVLENEQAPIEVAAMVRSVSSRGIAFKFLRSAQENKRLLAFVQSKTDSV
                     GPRSPA"
     gene            17318..17497
                     /locus_tag="PLS35_02540"
     CDS             17318..17497
                     /locus_tag="PLS35_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17558.1"
                     /translation="MTCGRCKGLMVDEWRPDFSPETFVWRCINCGSIVDPLIEQNRRT
                     RMAEQLLLEPVEVLQ"
     gene            complement(17566..18339)
                     /gene="xth"
                     /locus_tag="PLS35_02545"
     CDS             complement(17566..18339)
                     /gene="xth"
                     /locus_tag="PLS35_02545"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNI17559.1"
                     /translation="MKVATFNVNSLRKRLPIVLQWLERHQPDVLCLQETKVQDSEFPL
                     AALATSGYEITFRGMKAYNGVAVLSRTKPESVCYGFDDGGEAEDARLLRVVIQGIPII
                     NTYVPQGFEIDSPKYQYKLGWYDRLLKHFDMHLSPKEPAIWCGDMNVAPRPIDVHSPE
                     KHLKHVCYHEDARKAYGKTIAWGFEDVFCKLYPDRQQFTFFDYRAPSALAANKGWRID
                     HILATAPLAQKCQRVDVDLEPRRATDASDHTVLWADFDM"
     gene            complement(18468..18872)
                     /locus_tag="PLS35_02550"
     CDS             complement(18468..18872)
                     /locus_tag="PLS35_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17560.1"
                     /translation="MRRSIAVMVAAAFVGMSSFAAADDLGMGKMSEGMKQDMGTFSDE
                     MNAHKEELKSDLKAKKESAKSELKAKRDEAKAKKKQAKADAKAKKEHVKAKVKAKKDA
                     ARAKAADMKSAADDTVQEMGGMKNDLKEATRY"
     gene            complement(19066..19890)
                     /locus_tag="PLS35_02555"
     CDS             complement(19066..19890)
                     /locus_tag="PLS35_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17561.1"
                     /translation="MQDMLPVIGSSVFFDLLKSILLLLILLIARTVLVRWIKNNQALT
                     IDARRRWIVTTRNSMVLCLFVGLVVIWAHELEAFAVSLVALAATLVLATKELILCWSG
                     AALRVGGGVYGVGDRIQLGTYRGVVLEYDVFATKLLEIGPGQTSHLYTGRTVVFPNSL
                     LFGNPLIKESPSQEYGLYVLSVPLLSTDNWQAAEQALLGAAKVECAPFMDQMGRQMKL
                     LEQRNLLEAPSPEPRITIQLPEAGRIHLVLRFPAPDRGRSRVEQAILRRYLTTIRQ"
     gene            complement(19908..20399)
                     /locus_tag="PLS35_02560"
     CDS             complement(19908..20399)
                     /locus_tag="PLS35_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17562.1"
                     /translation="MTRNDNRLSYVSNRLLPLSVRWSRLGMLLCLAMLSGCGGPWRDT
                     YLNKGVHKLTQADIEDKFGPPSTAKTPVLGGDTVWTYRVPMGDREVHPWDPSSLAAGK
                     KTKAPPSPSPFGKSLAGNDEPYREPLYCYRYTLSFDEQKRLKDWKREECVPRTSEDGT
                     VVQ"
     gene            complement(20383..20598)
                     /locus_tag="PLS35_02565"
     CDS             complement(20383..20598)
                     /locus_tag="PLS35_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17563.1"
                     /translation="MSDLRTNALIARGMGIVFIAGGFMLGIYGLEFPNTMWLQTALGL
                     LVTGMMAQGYAMYCSIRWVREQRDSER"
     gene            complement(20670..21353)
                     /locus_tag="PLS35_02570"
     CDS             complement(20670..21353)
                     /locus_tag="PLS35_02570"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17564.1"
                     /translation="MRRPALVGLCALLLFMAWSCGQQTWEAAMQAGEVAVQRGQLGEA
                     EQIYAAALKKAEEFGPHDRRVAVTLAHLAQVYSTQGKFVEAEPVYLEALKIYQEVHGE
                     NHLDVAAMLNNLGVLHRKHGQFADAQRLLTRALAIKEKLLGPDHLEVALAVSNLAAMY
                     AAQGDTAQAAALFARALAIREKQLGPDHPDVAKSVIDYAGALRKIGRTPEADALEGRA
                     AAIRSKTKS"
     gene            complement(21350..22759)
                     /locus_tag="PLS35_02575"
     CDS             complement(21350..22759)
                     /locus_tag="PLS35_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI17565.1"
                     /translation="MPSQLSQKNERRHDVVVIGGGSAGYAAARTACEAGADVAIVDQG
                     PLGGLCILRGCMPTKTILRSAEIMALMKRAREFGLAPVTARADLSAIVDRKNRLVQEF
                     ADYRIAALRDPRFTLYESHAEFVSPHEICAGGLRIRGDAFVIATGSSVAKVAIPGLSE
                     AGYVTSDEMLDVRHAPVSLAVLGGGLVAVEFAQFFSRIGTKVTMLQRGATLLSDMDQD
                     VGQALASAFEDEGIEVLTGVAFDQVTSSPTGKTVHFRQNGVSHSRSAEVIFHALGRCP
                     NLKGLNLEAAGVKVIDGRLVVGADMRTSQPHIFAVGDVNDLTPIVHLAIQQGETAAFN
                     ATHPDVPARVIDHRLDSEMVFTDPQVAIAGLSERQCREQAIPYLTASYPFADHGKAMC
                     LGALAGFVKLLCRPDNGELIGAQIVGPEAAELIHELIAVMYFHGTAQDLLRIPHYHPT
                     LAEIVTYPAESILEQMGRI"
     gene            22809..23027
                     /locus_tag="PLS35_02580"
     CDS             22809..23027
                     /locus_tag="PLS35_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNI17566.1"
                     /translation="MIKPGRYRHYKGNDYEVLGVARHSETEEEYVVYRQLYGTGGLWI
                     RPMTMFSESVSIGGEIVPRFRRLEEGPA"
     gene            complement(23007..>23422)
                     /locus_tag="PLS35_02585"
     CDS             complement(23007..>23422)
                     /locus_tag="PLS35_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HNI17567.1"
                     /translation="LDSYRHCVGRELMERVDGAEAQAQALFTAPIVVVSHGMQDDPIL
                     NYGSQLALTLWEMTWEQLVHMPSRLTAEPVNRTERESMLERARTQGYIDNYRGVRISS
                     SGRRFLIEQALVWNVVDRAGYRRGQAATFSRWTFL"
     assembly_gap    23423..23522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23523..23877)
                     /locus_tag="PLS35_02590"
     CDS             complement(<23523..23877)
                     /locus_tag="PLS35_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17568.1"
                     /translation="MARWQWALLVACCSVMLASGSALASDRAHAKSLTRSGLTVPASS
                     LRSCSGEDLLGTWSLVAFDSAYRFKDPQAPYLFPYQVFQYSREQGAKSAHSRTPMLDS
                     PDRILEGVPLDLTYQV"
     gene            complement(23901..24134)
                     /locus_tag="PLS35_02595"
     CDS             complement(23901..24134)
                     /locus_tag="PLS35_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17569.1"
                     /translation="MEQEPINADHISIPRWTIKRDPRALGCSICGSAIVKITAEWRQV
                     SFCTCRLIEYRVLPRAPKALPAPSPAADSRKDP"
     gene            24296..25699
                     /gene="leuC"
                     /locus_tag="PLS35_02600"
     CDS             24296..25699
                     /gene="leuC"
                     /locus_tag="PLS35_02600"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNI17570.1"
                     /translation="MAAQTLFEKIWNDHVVRAEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LTVAGRTPRRSAAALAVPDHNVPTTDRRVAIADPVSAKQIQTLDDNCKTFGITLFGMN
                     DIRQGVVHVIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEVRVDGQLSPRCSAKDIILAIIGKIGTAGGTGYVIEYTGSAIRALSMEGRM
                     TLCNMSIEGGARAGMVAPDETTFNYIKGRPMAPKGALWDQAVAAWRNLHTDAGAKYDA
                     VVELRAETIAPQVTWGTSPGMVTGVDGTVPDPRSMSDDKLRQATERAIEYMALKPGMP
                     IRDIKIDKVFIGSCTNSRIEDLRLAASFAKGKKVAGSVHAMVVPGSGLVKQQAEQEGL
                     DRIFKESGFEWREAGCSMCLAMNADVLQPGERCASTSNRNFEGRQGAGGRTHLVSPAM
                     AVAAAIEGHFVDIRQWS"
     gene            25778..26398
                     /gene="leuD"
                     /locus_tag="PLS35_02605"
     CDS             25778..26398
                     /gene="leuD"
                     /locus_tag="PLS35_02605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNI17571.1"
                     /translation="MDAFTTLTGLVAPLDRLNVDTDQIIPKQFLKTIKRTGLREGLFY
                     DWRRRKDGSQDPEFFLNQPRYQQASILLTRDNFGCGSSREHAPWALLDQGFRCILAPS
                     FADIFYNNCFQNGILPVVLKAAEIQALFEGVAAHDGYTLTVDLAAQQVNTPKGMAYHF
                     DIDPFRKDCLYRGLDAIGLTLQHAAQIGEYEQRRRTQAPWLFQDVR"
     gene            26418..26807
                     /locus_tag="PLS35_02610"
     CDS             26418..26807
                     /locus_tag="PLS35_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17572.1"
                     /translation="MTWWETLLFPVAWLWRPKLLFTLLFFGGAAYLLIVFNWSYSDGD
                     RAGYLQKLSRKGWFCKTQEGELAMTTVPGVAPVLWSFTVWDENVAKKLDGQMGKRVVL
                     HYKEFRYIPTTCFGETPYFVDRVEVLD"
     gene            complement(26817..27875)
                     /gene="corA"
                     /locus_tag="PLS35_02615"
     CDS             complement(26817..27875)
                     /gene="corA"
                     /locus_tag="PLS35_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNI17573.1"
                     /translation="MKLKHKHSRKAGLPPGTLVHIGEKRSETVTITVYEYGEGQYQER
                     TVSKPDEVVATGEPTVRWVDVGGIHKMEVLEAFGKMFGLHPLLLEDIANTDQRPKLDD
                     YGTYGYLVLKMLYEGQSEGDINVEQVSLVFGENFVLSFQENGGDVFQGVKERLRNGKG
                     RLRHAGADYLLYALMDSIVDRYFVILETLGEKIEALQDLCVTNPGPETLRDIHALKRQ
                     LLFFRRSVWPLRDVMNNLSRSDGQFLQGSTKVFFRDVYDHIIQIGDTIETLREMVSSM
                     MEVYLSSVSYRLNGVMKVLTIITTIFMPLSFIASIYGMNFEHMPELKSPWGYPAVLGV
                     MALTGLAMLYFFKQKKWL"
     gene            28017..28820
                     /locus_tag="PLS35_02620"
     CDS             28017..28820
                     /locus_tag="PLS35_02620"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNI17574.1"
                     /translation="MEYELTILSQAMSEAGREALRLASAGFETFTKADQSPVTSADLA
                     VNRILQDRLSTAFPEDGWLSEETTDNDTRLSRKRVWIVDPIDGTRSFVRGLPEFCLSV
                     ALVENGVPTVAAIFNPATGEFFSAIRGRGLRVERLSDSDQPGFTSSERPVALVNPWEL
                     RVGRFEGLQDHLHCRPIGSIAYALALVAAGQADAAITLAGGNEWDVAAGVLLVEESGG
                     RATTAAGHPVTFNRPDPRLPGTLAVGSDLPAPVRTRLIQHGLAAASHPL"
     gene            28851..29759
                     /locus_tag="PLS35_02625"
     CDS             28851..29759
                     /locus_tag="PLS35_02625"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI17575.1"
                     /translation="MAALATSERYSMNVALLGTGLLGQGVAERLHATGHDLTVYNRSI
                     KKTQALRQQGIRIALTAAEAMSAADVVLLLLSDAAAIRAVLFDPAASRAIDGRTIIQM
                     GTIGPAESCSLEKDVTGLGGRYLEAPVLGSIAEAKAGTLLVMVGATPEHYDKWAPLLR
                     YLGEEVRLIGPVGKAAVLKLALNQLIAAEMAAFAFSLGLIRRGGVDVNDFMGVVRKSA
                     LYAPMFEKKLSRLEKRNYAQPNFSTRHLLKDVELALSAAEKARLSPAGLQGVRSLLID
                     TIAAGLGDVDYSAIYEQIDPLGSEHG"
     gene            complement(29763..30239)
                     /locus_tag="PLS35_02630"
     CDS             complement(29763..30239)
                     /locus_tag="PLS35_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17576.1"
                     /translation="MKQKQWFFLVIGLSLLSIQACSHAPRPQETTWTEPPAGEISLPR
                     GEHAVLAGEWEYEEGGMVVPLRLDRFGNGSYDFKDGRFRTDLFSDHRWAGAWAQREND
                     REGGFEITLSPDYSEGEGRWWYTRIEGDKAPTKAGGRFHVMKVQSIVDNQPWPSAR"
     gene            30590..31048
                     /locus_tag="PLS35_02635"
     CDS             30590..31048
                     /locus_tag="PLS35_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17577.1"
                     /translation="MPSSDNKHAGGEAQNPSHSSPLNILLVEDMEDNRVLVSLLLKAF
                     PYRLDLAEHGAAGVEKFQSGRYDLVLMDIQMPVMDGYAAIRLMRQWEKEQQRSETPIL
                     ALTGNTHDEDIGKAQAAGFTAHVMKPLKKVTLLEAIQRYAAPFSRKESAA"
     gene            31045..31380
                     /locus_tag="PLS35_02640"
     CDS             31045..31380
                     /locus_tag="PLS35_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNI17578.1"
                     /translation="MSDMAPVTSSCGAGPIVVLVDSAFEPLIPKFMANRNKELATMTE
                     ALATSDFATIRQLAHGMKGVSGSYGFHAMTTIAGRMEEAAKVANGTSIRQDLLELASY
                     LTRVEITYE"
     gene            31589..31984
                     /locus_tag="PLS35_02645"
     CDS             31589..31984
                     /locus_tag="PLS35_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17579.1"
                     /translation="MDNPETPKLRVLMVEDEEDTASLLKFLLERANYQVVHAKDGRQA
                     QELVNTIAPPDIVLLDVMLPFLSGLQVLTVIRKREGWKKVPIVMLTADGSEHDIKRAL
                     ESGANDYMIKPFNPRELTSRLKRFVAHAA"
     gene            32006..33097
                     /locus_tag="PLS35_02650"
     CDS             32006..33097
                     /locus_tag="PLS35_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI17580.1"
                     /translation="MLGLDQSDLTLRIGTIAAISIYSSVLLLLLGILLLRYIRNNKDR
                     RRRAVTEIWRPILAECVVEVPETLPPLTTRDHLVLLYLWNHCYESIRGDARENLKELA
                     RRTGTDRFAKELLHARLLRRRLLAIVTLGHLQERSIWNELAAALQADNAFVSLVAAKA
                     LLHIDAKAAVPLIIPVISQRKDWSPLKVVAIFEPAGADIAAEAIAKAACLASPEIGSR
                     LIRHLAATQSQHALPPLRDMLQKGTPSDDLLAACLFLFGECSDPRDIVTIRTHLSHPT
                     WYVRLQAASALGKVGIEEDEARLIALLDDEHWWVRYRAAEALSNLPWMTTERLTRVHN
                     TLTTVESQEHLVPFMAQSNAKAQHPARIA"
     gene            complement(33125..35080)
                     /locus_tag="PLS35_02655"
     CDS             complement(33125..35080)
                     /locus_tag="PLS35_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17581.1"
                     /translation="MQRSTLVRVSGIALLFVSLVIGVRILVSAPPVQHMEVPEKDFHR
                     FQGVAGPDDATVVPRHVPTAWTTYEPGSTSRLAILLTDPDSSWLGLAHGLKSIGIPFR
                     ITRNVHEALAHKVVLVYPMISGKVLKPEELQALAEFPKTGGTLIGVQVLGGGLNHVFG
                     FRDAVASRQRYDMTLATADPLLADLTDPREQRLRVGIRDKGIEVMGTYGYTDASAPLA
                     RFDDGTAAVTQVAHGPGRAYAIGVDLGFLLLKGYNNRGEELVKTFDNEFDPTLDVWLR
                     LIKAMYRTAQEEAVTLGTVPFGKSLSVMMTHDVDFTQSIKHAVEYAEYEKRQGVVGTY
                     FIQVKYIRDYNDDIFFNDEGVRYVKQLADLGMELGSHTIAHSRVFNQFPMGSGRESYP
                     SYAPFVKDRTTAYNVSILGELRVSKFLIDHFSGQSIVSFRPGELSNPFGLPQALLATG
                     YRYSSTATANNSLTHLPYQLNYDRLTDSEVEIFEFPVTIEDEALPKMGDRLPQALEVA
                     KRISRYGGSVVVLIHPNVLDHKLAFEKGFVEGVRTYAWFGSVGEFGRWWAARNRVEVD
                     VVRDADAMRVTLNVPVSLAGLTLEVPPGWEVARSGNPLRGVAQHGTMVTFPQLQGTYT
                     VLFTPSSLTPSASRTSSSRSNTVNMGL"
     gene            complement(35085..37448)
                     /locus_tag="PLS35_02660"
     CDS             complement(35085..37448)
                     /locus_tag="PLS35_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17582.1"
                     /translation="MNDSFTWINQRTSVAGLLAVIAFAAAWFFAPGTMIAAMAPELSP
                     RQTLQQARVLEDEKRYEEAIAAYRQYLQAKPDDDDVRASVAKLLSWQGQWDAAAAAYR
                     DLLTRHPLDHDIRVGLAQVLSWQKHYDLAREEYERVLHDEPDHVEALTGLGNVYLWSG
                     HQDQAIPYYERVVALTGDADVAARLQSLKAASRSPVVPVNPSGAADGNDSLTAHAAQT
                     LERGGRLETMRQYADAVTVYREELQLSPANDEVRAALARVLSWQGVHPEATTLYREIL
                     ARHPQDQDIRVALGQVLSWQKQFEEAGRVYQDVLQSDPTHVEARRGLADVAHWQGHYA
                     EAVQRYEALVAETHDPELEAQLKTIRSEWRTTSPQVAEHPASTHDSQGTDERVIGAAV
                     EEATKFEVAKQYPEAERVYRDVLRRHPDNDDIRTALARVLSWQGAHAEATTLYREVLA
                     RHPEDQDIRLALAQVLSWQKQFDEAERLYGAVSQAEPTRIEARRGLAEVAHWRGDRSE
                     ALQRYEALLDDTHDPAIEQQIKAVKSELLVSPRAAVGQGLAGLRLPYRDYAKIGYGHY
                     TYTKNQPDERDLLFELAKPLGNQTLVMRVEPINRFGFHDTPMSAELYSPLWSRAWGYI
                     AGQATINPHFSPNYSFVGEVAQGLGGLHSSLAPFEASFGYRRLNYKHDDIDLLMPGLT
                     IFLPFNLWLTEKVYFIPNTGAVTLASQLTWRPADRLQFFASGSFGTSGERIVAAQDFT
                     RVGSRTIQGGVTFPINERFSAETSGYYEDRGFLYVRRGGNFNLIYHW"
     gene            complement(37482..39158)
                     /locus_tag="PLS35_02665"
     CDS             complement(37482..39158)
                     /locus_tag="PLS35_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17583.1"
                     /translation="MSVSTHIDQCVSARDGVARDQIDGKSVGRGTEAVGRRLFATVLC
                     SAAVISILWLSLAQAEDIPLSPPIPSTIHEIELTYAGNSTDPAVAFSGPVPPAGPLPY
                     RPFLPILELLPGFGVAHVFGPCHGQAVRSDSHLELEWTAGDAGRCGQRIELRPAGRPM
                     DLMSYQMLRLRGRATGQVVVAIEDLAGHQREHNQSLATVTGTFDLTIPLKEIGRGVDL
                     RYVTALVVSAEGPRGRIEFEQLTVSQRNTVKKPPGGTGFWVWNYRAAIQEPQPLLATC
                     RRQACSRMLIQMPSLNDDEAIWEGYASLMAQVQEAGIDALALDGYPEAIQEPHLLADK
                     VQRLFHLVRGGAVSGIQLDIEPYLLPGFLDDDGQLRRYLGAIETVSEAIHGRAKLSMV
                     IPFWLATPTVGGRPLAYAVMDRADEVAVMSYRTDLEEVQDIAEDILRYGDVSEKPVWL
                     AVETTVLPLEEHVVLRRDLRPDHADALLDREHKRLRWKPISEADRLTGSGEWFRIHRR
                     FTVRPERLTFAGRSRVEVSQAISHMLDTTMHPSFAGVIIHDLDGFRALPE"
     gene            39139..40545
                     /locus_tag="PLS35_02670"
     CDS             39139..40545
                     /locus_tag="PLS35_02670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI17584.1"
                     /translation="MWVDTLISTSEWLFLLYFLGLTAGYLMLDLIAIVHLRQYLQERV
                     LESLPHSYTGFEPQISILVPAYNEAATIAGSIRSLLQLSYSEYEIVVINDGSKDATID
                     VLRKEFDLFPFPEACNTQLPTAEIRGIYHSSSHTNLRVIDKENGGKADSLNAGINLSI
                     YPLFCCIDADSILQPDSLRRAVQPFLDDHRTVASGGTVRIANGCRVENGFLTKIGLPS
                     NLLALFQIVEYLRAFLFGRLGWSPLNAMLIISGAFGIFRKEAVVTVGGYRSDTIGEDM
                     ELVVRLHRHLRMGDKPYRITFVPDSVCWTEAPEDLRTLKNQRIRWQRGLCESLTKNLD
                     LLCHPKGGTIGWLAFPFMAVFEWIGPAIEVFGYCFLIFSFVMGFVSIQAWLILLVAAI
                     GLGVLLSVSALLLEEMSFHVYPDQRDVLKLLLAAILENFGFRQLNSLWKLIGLYRWVC
                     GKKATWGTMTRTASWQAR"
BASE COUNT         8615 a        12325 c        11352 g         8090 t
ORIGIN      
        1 tctacacacg cctccatcgg caactccaag aggtgacgga ccggttcttt cctgaaaccg
       61 agtggacaag tatcgatgaa ttctatgcgg acacgacccg gctacaaact cgacatccgg
      121 atccccgcgc acttggcgat gccctgaaag ccgacattct ccacacgacc ggcttgacct
      181 gcacgatcgc cctggcttcc ggaaaaaccg ccgccaagat cgccgccgac gcacataaac
      241 ctgacggcct ggcggtgatt gaaccgggcg cggaagccgc atttctggcc ccgttgccga
      301 ttcgctcgtt gcccggcatg ggtcccaaat ccacggaggc cgtcgagcgc ttgggggtgc
      361 ggacgatcgg cgatttgctt ctccctcgat tcgaatcagc cctcgctcgc ctcctgggca
      421 cccgcctggc gtcgtttcaa tcgctggcac ggggcctcga ttccgatccg gtggtcgctg
      481 atcgcgaagc caagagcacg agtcacgaga cgaccttcga cgaagatacg aacgatccgg
      541 ctgtactgga gccgatcatc catggatttc tcgaaacact ggcgcatgag cttcgcctgg
      601 agggtctggc tgccggttca ttcaccctga agctcaagga ttcgcatttt cgcatcacca
      661 cgcggcaacg gaagtttgcc acgccgttga actatgaccc ggacatgtgg ccggagattc
      721 gccaggcctt gggagatttg cttcagccgc gattgcggta ccgcctggtg ggcctgggcc
      781 tatcggatct ggtaccggct ccggaaccgc tgttcgaccg ccgtcaacgc gatgcggttg
      841 ccgccctgga tcgactgatc gaacgacatg ggaccggcgt cgtgcgtctg ggcgccctcc
      901 cgcagagtga agctgggacg aggaaacgaa gacctcgcac gccagactga ttcccgagcg
      961 caccacgttc gccatgcatt cctggtggca gactttttct ccatcctgat aagatgcacg
     1021 cgaggattga ttgaacgtga tgcctacaaa agtgaccacg cgagatgaac tggtttctct
     1081 gctggccgag cgccgccagc ggcaacagcg cgtcgtcttt acaaacgggt gtttcgatct
     1141 catgcacatc ggacatgtcc ggtacctgca ggcagcccgc aatctgggag atctgctcgt
     1201 ggtcggcgtg aactccgacg gttcggtgcg cgcattgagc aaaggtcccg atcgccccat
     1261 tgtcccggac gtccaacggg ccgaggtcct cgccgcactg gcctgtgtcg attatgtggt
     1321 catcttttcg gagccggatc ccggagccct gatcgcagcg ctgcagccgg atattctggt
     1381 gaagggggga gactggccgc ttgaccggat catcggccgt gaaaccgtcg aggcacgcgg
     1441 cgggcgcgtg caaaccattc ctctggtccc cggcgtctca accacgaccc tggtacaacg
     1501 tattcgctcc accacggcat aaggatttcg tgccacctgt cgtcccatcg catctcacga
     1561 actggttcgc gcctgcgcga gaggccctcc gcacgggatc gggcaaaccc tgcttgatgg
     1621 gattgcacgg ctccacggca ggcttcgggc tcgcgctcct cacgctcagc gcgccggcac
     1681 aacctctcgc cgaccgctcg tggctggttg tcgccaaaac ggatgacgaa gcggagcggc
     1741 tctatcgcga cacgcttttt ttcagaaccc tctgcggcca atcaggcgat gacctggccc
     1801 tgttcccgaa atgggaaacg ctgccctacg aatcgacggt gccccacatc gacctcgtct
     1861 cccgccgcat gcaaacgctc aatcgactct gcaccacagc tcgcacggtg ctctttacgt
     1921 ccgtgccggc tctgacgcaa cgtgtgcttc cagccctggt cttcaccgaa tccatactgc
     1981 agtttcaacc gaacagcgat ctggagcgcg aagtgctggt ctcgagcctg ttgcgcctcg
     2041 gctatcgcaa gggatcagtg gtagagatcc cgggcgaatt cagtattcgt ggaggcatcg
     2101 tcgacattta ttcgacggcc tatccggacc cgctgcgagt ggagtttttg ggcgatacga
     2161 tcgaatcgat ccgctttttt gatccggcca cgcaaaaatc gaccgataag atcaagcagg
     2221 cctgggtcct gcccgcgcga gaactgattc gctcagacga tgcaccagac gcgcttacgc
     2281 ccttggccgc agatgcggaa tggcatgccc cgtcggtcta tggggcgatg gacagcctgc
     2341 tggattattt tccccaaccg cccgtgctgg tactggatca gcccggcgcc ctcaaagctc
     2401 acacggcaga atgttggcag accattgagg aagggtacct gcgtcacgaa gatcgcacgg
     2461 atccgaatcc ctatccgact ccggaccagc tgtacctcac gtgggatcaa ctcgtcaccg
     2521 ccactcaaga ctatgcgacc cttgccctgg agccggtcac tgcgccggat gcgagctggg
     2581 aaccggtgct gacctgtccc gcgcaaacac cggccagcgt gggactcggc cagcgcggca
     2641 cggcgtttag tcagacattg gaagtcctcg atcggctgcg cgaaggcggg cccgtggtgc
     2701 tggtcgcccg gagtcagggc caagtcggcc ggctgctcgc gctcttcggc gagcacgatc
     2761 gaccggcgat ggagtggaaa cccgcggccc tttccgcagg cggcgcgcag aaggccccat
     2821 tttcggtcct gaacggagag gtctcgtcgg gattcctttc gcccgacttg cggctcgtcg
     2881 tgctgaccga agaagaactg ttcgcaaagg gtgcccggca caaaccgcaa cacaagagca
     2941 aagcggccac gtttctctct tcgctcgaag atctgaacat cggcgatttc gtcgtccaca
     3001 tgcagtacgg catcgcgaaa tatcagggat tgcgccggct ctcggtccag gacttcgaca
     3061 gtgactttct ggtgctcgag ttcgccggga ccgataagct ctatgtgccg ctcgaccggc
     3121 tgagccaggt gcagcgctat gcaggggccg acgcgcatgt gccgcgcctc gaccggctcg
     3181 gcgggacgag ttgggcaaaa acgacggcgc gagtcaaaaa agatatcgaa gaaatggccc
     3241 atgagctcgt cgatctctac gccaaccgcg agctggtcca tcggacgtcg tacggcaagg
     3301 acagcatgct gtatcacgag tttgaggcgg ccttcgaata tgaggaaacc ccggatcagc
     3361 ggaaggccat cgaagatatt gcgaaagatc tggcctccac caaaccgatg gaccgcctcg
     3421 tctgcgggga tgtgggctat ggcaaaacgg aagtggcgat gcgggccgcc ttcaaggccg
     3481 tcgaagaaaa ccggcaggtg gccgtgctgg tcccgaccac gctgcttgcc catcaacatt
     3541 acgacaattt cgccgaacgg ttcgcgccct tccccacccg cgtggcgctg ttgtcccgct
     3601 ttcaatcgcc caaagacacg aaggccatca tcaaggatac ggcggcgggt ctggtcgatg
     3661 tgctcatcgg cacccaccgg ctcttgcaaa aggatgtgca gtttcgcaac ctcggcctgg
     3721 tcatcatcga cgaggaacaa tggttcggcg tcaaacataa ggaacgctta aaacagctgc
     3781 gtacccaggt cgacgtgttg acgctcaccg ccacgccaat cccgcgcacc ctgcaaatga
     3841 ccatggccag cgtgcgcgac ctgtccatca tcgacacgcc cccgtccggg cgccttgcga
     3901 ttcgcaccca agtgcttcgg ttcagcgaaa aagcgatccg cgaagcgatt ctccgcgagc
     3961 tcggccgggg tggacaaacc tacttcgtcc acaatcgcgt ggaaaccatg gaacggatgg
     4021 gcgcgtggct ccaggaactg gtccctgagg ctcgcattgt gatggcacac ggccagatgg
     4081 attccaaacc cctcgaagcc gtgatgttga aatttttcca ccgggaagcc gatatcctga
     4141 tcgcgtcggc gatcatccaa tccggcatcg acgtgccgac cgccaacacg atcatcgtca
     4201 atcgcgccga tacgtttggc ctggcccagc tgtaccagct gcgcggccgg gtggggcgtg
     4261 gtggtgaaca ggcctacgcc tatttcctgg tgccggatga aggcaatctc tcggacgacg
     4321 cgcagaagcg gctgaccgcc atccagcaat ttaccgagtt ggggtcggga ttccggattg
     4381 cagcggcgga cctggaaatc cgcggggcag ggaatctgct cggcaaacaa caatccgggc
     4441 acattgccgc cgtcggactc gatctctacc tgcaaatggt ggaacaagcc gtccagcgcc
     4501 tgaggggcca ggtcgtcgag gaggagccgg accctacact gcgtctcagc gtctcggcct
     4561 acattccgga agactacgtg gtcgacagcc atcaacgcct ctcgctctac aaacggttgt
     4621 cctcctgcgg acaactcggc gacctcgcgc tcatgcatgg cgaaattgaa gaccggtatg
     4681 ggcctccgcc ggacccagtc gaacgactgt tcgaactcat gcagatccgg ctcctggcca
     4741 aacaactccg gctcagttcg gtggtcgagc agacgcatgc ggtcgtgatc accttcgatc
     4801 ccaaggcgaa agtttcggaa accgctgtgc aggccctgat ggaccggtat aaaaaacggc
     4861 tgcgatttct gtcgcctttg tcgtttgaac tccagatgcc gcatgacgac tggagtttgg
     4921 tctttccaga actcaacgca accttgcaaa cccttcacgt ctgtggtacc aacaatcaag
     4981 agccacggac cggctctacc tgatcttccc agaaggatgc tccgccccga ccgcatgaca
     5041 tcaccgcaga caccagacat atccccctgg cgggcacgac tgaccggcac cgcgctagcc
     5101 ttgtgtgcgc tgggctcatt ggccggctgt accgaaccgc cgcaggagga accggtgatc
     5161 gcgatgatca acggccgctc cattacccaa gctgagtttg acatccggtg ggaagaactc
     5221 tcccaggcca cccgcgcccg gtatgaaaaa gaaggcggga aacgccgctt cctcgatgaa
     5281 ttgatcatgc gcgagctgtt gatgcaggaa gcgcgcaaac agggcttgga ccaatcggat
     5341 gacatccggg agaaaaccct gcgctatcgc gagcagctga ttctcgatga actcctgaaa
     5401 gaccggatca agaccaaggt tgaagtgtcc aaagaagaat tggatgccta cctcgaaaag
     5461 catgccaacc agctcctggc gaatcccaaa gttcaggttt cggtcatgtt gctacccaac
     5521 gtctatgcgg ccaaggatct gaagcgccag gtggaaggcg gcgggaactt cacccgcttt
     5581 gccctgcgct attcgatcga cgaacgcagc cgcgccaaag gcggcgacct cggcccctat
     5641 cgaaagggcc tgctggagcc tgaactggac gcgttgattc cgtcgctgca ccccggcgtc
     5701 ctcagcgatc cgatcaagac cgacaagggt tactatctga tgaaggtcag tcccctggag
     5761 ccggaaattt tacaggccga ccaggccacc cgtgaacggc ttcgccagga actcctggct
     5821 gagaagcggc ggaaacgcct tgatgatgtc tttgccgaac tccgcaccgg tgcgacgatt
     5881 cgcatggctg atgcggcccg ttatgtgacc gatgaaccag ggcgccccta acctcctgcg
     5941 caacggcttc ctgcccgcgt tccgtgttca cgcctccttc cttttcatcg caagatcgct
     6001 ccctttctga agtctcgatt gtgtacaatc cccttaccgt gatgaatgcc cgattgtctt
     6061 tcctttctca ttgtgtccgc gccgtgagac caacctctct gcaatcgctc agccgaccgg
     6121 ctttggccgg cctatgcctt tacgtctggg tagggctgtt cccggccggt ccggtcgatg
     6181 ccgcgaagtt ggaagaccgt attgtcgcag tcgtgaatac cgacctgatc atgctctccg
     6241 agctcaagcg cgacttgctt cccgatcagg agcgccttcg caagctgtac agcggcgagg
     6301 atctcgaacg ccgcctcaag accgcggaag cgatggccgt cacgaagatg atcgagcgca
     6361 agctgcagct ccaggccgcg aagaacaagg gcgtggaggt gtccgaccag gaagtcacgc
     6421 aggccgtgca ggaaatgaaa aaacagggcg aagccctcaa ccaggcagac cccaacacca
     6481 ccagaagcgt tcgtgaacag ctgacgttga tgcgtgtcgt cgaccgggaa gttcgagggc
     6541 tgattatggt cgccgattcg gaaatgaagc ggtactacga ggagcaccag gatcgtttcg
     6601 cctaccccga ggaataccag ttgagccaaa ttctcatcaa accgcggaca ccggacggcc
     6661 tctccgtggc gcagggtcgg gccgaagccc tcctcgcgac gctcaaacaa ggcgaaccct
     6721 ttgaagatct ggcgctgcgt ttttccgatg gcgcagacgc ttcgcgcggc gggcggttag
     6781 gactggtccg gcaaggggaa ctgattccgg cactggagca ggcgctcacc tcggtgccgg
     6841 tggggcagat tacaggaatc gtagaaacgg cggaggggct gcatatcgtg cgcgtcgatg
     6901 acaaaaagcc gcgccagttt cgcccgttcg aacaagtgaa ggcggaaatc cagtccctcg
     6961 tgtttcagca gaagaccgaa gaccaatatc agatctggat ggccgatctg aaaaacaagg
     7021 cctacatcga gatcaagttt taacaggatg ctgaaaaagt ccgccagcag cgttctcgcc
     7081 tcgttcagac cctcaacgta cctccaaggg tacgcttcgg cccttcactc gctgcggcct
     7141 tgctggacgg tctttttgag catcctgcgg gagtgttctt ttgcgatgtg atacaagcgt
     7201 gctatcgaag ttctcttgtg ccagattcgt gtttctgcaa tccattaaac tgcgcgcgaa
     7261 tctcgcccca taacgcgtcc cgcccctcgt gggtttcggc cgaatacaaa agaaccggca
     7321 cttccgccgg cagcccgcaa gcgacacgca cttcggtgag agtcggtttc cgctcgctct
     7381 gtcgcaactt atccgccttc gtcagcacga tgatgaggcc acaacccagc gactgcaccc
     7441 actggatcgt catgacatct tgcggcatcc agacgcgcga ctcgatcagc agaatcaccg
     7501 cgccgagcga ttgccgctgt tccagatatt gttcgatcat cgggccccat tgcgcgcgca
     7561 tcgttttgga gaccttggca tagccatatc ccggcagatc gaccagtgac aagagcggca
     7621 gcttcggatc ggaagtacga acggtaaaaa aattgacggc gcgcgttttg cccggcgtct
     7681 tgctgacctt cgccagtttc ttgcgatgca agagcgaatt gatcagcgag gacttcccga
     7741 cattcgatcg tcccacgaac gcaatttccg gcaggtgatc ggcaggaaac tgttctgggg
     7801 acacggaact tttgataaac tcggcgctga gtagtttcat aatgatcgcg tcaggcagca
     7861 atgcgggatg ggcgccaact tgggccacag aggacaagtg agccatctcg taggctcata
     7921 gatagagtgt agggaatgag caagtcaaca ggcagtctct tcgggcggat actgttttgg
     7981 atgaccgtgc tcatcggttt gccggccgcc gccttggcgt tgtattggct ggccacattg
     8041 cccgacgtct cgcggctggc gaaacatcac cccacagaaa cggcggtgat ggaagcccgg
     8101 cgggcccaag ccaaggaaca aggccatccg ctgcacatca aattcgcctg ggttcccctg
     8161 tcccggattt cgcccgcgct ccaacgtgct gtcgtcgcgg ctgaagacgc ctcctttttc
     8221 gcgcacgaag gatttgactg ggaaggcatc aaggacgcgg cgctctacaa tctggaggtc
     8281 ggcgaattca aacgcggggg cagtaccatc acgcagcagc tggcgaaaaa tctctacctg
     8341 tcatccgaac ggtctctgct gcggaaagcc cgggaggcct tgatcacccg ctccctggaa
     8401 caccatctga cgaagaagca gattctcgaa ctgtatttga atgtcgccga atggggccag
     8461 ggcgtctttg gagcggaagc ggcggcgcgc caccatttcg gaaagtccgc cgaggatctc
     8521 acgatcgagg aggcagccct gctggccgcg attcttcctt cccctcgtcg ctatgacccg
     8581 attcggcaca ccgcctatct cactcgacgg caacgccaca tcgttcgctg gctggagaga
     8641 gggaacggcc ggaaagcggc ctcgatcaac cgactccctc cggaaagtct cgaagtcgtt
     8701 cctgtggagt aataccattc actcgtcacg cgcaacgcca gcactatgtg gattggccga
     8761 ccgacgtgta gtcgcgctct ccgaaaatcg ccgactcgtg gacgtaatac ttaaattcga
     8821 gcagatttcc tgacgggtct tccaggaaaa acgtgcgatg ttcgattcgt gtcccgggaa
     8881 accggcggcg tggctgttga taaaacgtaa ggtgctgggc ctccgcgcga tcggccagcg
     8941 cctgccattc ttcctcccgc gtcaacacca gcccgaaatg gcgaggatag atacccttct
     9001 gccgatcgag ttcgtcttca gagaggtggg cgacgagttg atgcccggcg aggccgaagg
     9061 tgacggccgt tggagattcc cgccccagga cacacccgag cccctcgacg tagaattgtt
     9121 tggcagcggc caaatcgtgg acgggaaagg caagatgaaa aagcgccggc ataggctgag
     9181 acctcgtgtc actgtatcag ccgatgactg cgctaggcaa accggtcgcg cacaatcgcg
     9241 cgtccgtccg ggccgatgat cccggcaatc atatgcgggg tgctgtggcg aatcgtgcac
     9301 cggccgtccg gcgagagcac taagacgccg gctgcacctt gaacgcgctc cactactttt
     9361 cgcagcacgc ggttggccgc ggccaccggg ctcagaccgg tcgccaaccc atccgcaatt
     9421 tccttggcga cggcaacccg aatgatgctc tctcccaatc cggtcatgga tacggcaccg
     9481 gcgtcattgt cggcatagac cccgcagcca atcagtggcg tatcgccgac ccggccaggc
     9541 aacatcacat cgacaccacc ggtcgacgcg ccggctgcca cggttccctt cacatccaac
     9601 gccaccgcgc ctaccgtttc ttttcctaag ctccttgcac gaaggcggcc ggttttcaca
     9661 atggcctgat gcagctgcaa cgttcgactc acccccgctg ctggtctcaa ttgcttgggc
     9721 tgggctgtca ggcttcgcct cgaacgcgga gcgcgctcga gtccaaaata gtctgcaaag
     9781 cgactggcgg acttcccgac cagtaagaca tgcgcggttt tttccatgac gagccgggcg
     9841 gcggtgacgg gatgaaggat gccctcgatg gaagccaccg cgcctgcgcg aaggtcccgg
     9901 ccctccatga tcgaagcatc catccggcgt acgccgtcga gttggcgatt ggagccgcgc
     9961 cccgcattaa acagaccgga gtcttccaac aatcgaatgg cctcttcgac cacagacaac
    10021 gccgggacac cggcctgcaa cagacgatgg ccttcggcca acgtgcgttc cacgcaggcg
    10081 gcttgagcaa cggtcatggc gcggcgtccc gcgccaccgt gaacgaggag aaccggtcgt
    10141 aggcgtttca attgaggagc agatctttca cgcgacggca ggcaggatct tgcatacggt
    10201 ccaagtcaga ccggacaaat cccagtccgg tcacacagag ttcaaagttt tctgacagct
    10261 tgcggaacaa cggagtatcc tgctcgggat cgtaatgacc aaattccgcg accaggccat
    10321 aggaccgctt cccggctttc acgtaatcga ccagaaaatc cttgtgatag attctcccca
    10381 cgctcttgag gcggcggaga tattccggaa agagcccggt cataaacagc gtaaagtcgc
    10441 cgatgtggcg atgcacgtca cgctcacgtt cgaacgactg tgcctccaac aagacttcgg
    10501 actcaaataa catctcgacg accgagtcga gcggacggcc ctgggtattc tggattttgt
    10561 agagttgatc ggtgtgcgcg aattcgacga gcaggttgga gacgtactcg gtgacgttca
    10621 gatcgggcca cccgaggtgt tcggtgaaac ttttctccgt caatgctccg aagagctgcc
    10681 ggagcggatg gctgcgagac acgggacttc ccatctcgac ctcccgggca atccttgccc
    10741 tgtgaaaata gtataccaga ccattctcaa tccgagcgga ctttctctct gactccttat
    10801 cggcatcccg gatcaagact tgaggtccgc gtgtgctccc ggtcgccggc tatccacaat
    10861 aaacgtgact ggcccgtcat tgagtagttc cacctgcatg tgcgcgccaa agacgcccgt
    10921 ttccaccttg aggccttcgt gacgtagtct ctggacagcc tcgtcatacc atgcacgcgc
    10981 cgcctccggc ggagcagcta ggtcaaaccc cggccggcgc cccttcgcgg tatcaccgag
    11041 cagggtaaat tgcgagacga ccaacagcgc tcctccgaca tcacgcaggg agcggttcat
    11101 ttttccctga tcgtcattaa aaatccgtag ggcttgtacc ttctccacaa gataggagag
    11161 atcgccttct ccatcaccct tggccacgcc caataacacg agcagtccga ggccaatgcg
    11221 accgacgacc tgcccttcta cctcgactga cgcctgtgtg acacgctgga tgaccgcctt
    11281 catctcgagt tcgccacaat cgttcgcacc tatcgggcat tgcgcaattg cgtcaatgtc
    11341 ccttccaaac ccaacagacg ccgcttcaac ggtaatccgc tcgaaaaccc gcccaaggag
    11401 ccgtcatgcg caatgatgcg gtgacagggc acgacgatag gtaccggatt cgctcccagc
    11461 gccatgccca cggctctggc atactgcttc ccacccacac gcatggccac ccactgatag
    11521 gatcgcacgc ggccgtaagg aatccctgca atcgccttcc acacctttcg ctgaaacgag
    11581 gtgccggcag accaatcgat aggcagcgca aactcgcgac gctccccgtc gagataagac
    11641 aagagctggg cttgcgcctc tttcacaata gacgccgcgg cctcgtctct gtgtgaatca
    11701 tgacgaacgg tgcgaacaga cggaaacaaa tcaatggacg tcactccctt tgcagaagcc
    11761 gtgacggtca tccaccccca agacgtttta aaggtcctcg tctccgtcat ttcatgcgac
    11821 cccgaatttt cgcgcgaccc ggccgagttt acgcttgacc aatcccatga ccacatccag
    11881 ctcgtccgac cctagcatga gatgcatgcc gagatacgcg atgacgctga acccgatccc
    11941 cccaaacagc atgacggact tcacgatcca ttgaccggtt tccatccaca ggggcgcgct
    12001 cgccacccac agacagatgc cggccattgg aatggaggcc gccagtaccc ggccggccga
    12061 tcgcacgaca gcaccccaat ccacccgccc caagcgccga tgcaacacgg ctacgagaat
    12121 acttccgttc agcatcgagg ccaatgccgt ggccagcgcc aagcctgcgg cgtccagaat
    12181 cgtcatcaac cagagagaca agccaatatt cgccccgacg gcaatccccg ctgttatggc
    12241 cggagtccgc gtatcctgca gggaataaaa ggcggacacg ataatacgga ctccagcaaa
    12301 tgcccagagt ccgacggcgt aacagagcag cgcggtcgct gtcgccaggg tgtcggtctt
    12361 ggtgaaggat ccgtgctcaa acaccaaatg cacgatcggg taccgcagga ggatcagccc
    12421 ggccatggcc gggaggatga tgaaaaaaat catcctgagg ccgaacccga tggtcgtccg
    12481 caattcatcc agtgctccgc gcgcagcctg cgccgacaga gtcgggagaa tagccgtggc
    12541 gagtgctaca ccaaaaatac ccagcggaaa ctgaatcaac cgcatcccgt aaaacaaata
    12601 cgtgggcccg ccggagaaat aggacgcgag gatcgtgctc accgtgatat tgatttgggt
    12661 cactgacaag cccagcagtg acggcaccat cagaagtccg atgcgtttca ctccgggatg
    12721 gcccgggtta aaccgcaggc cgaacaacat gccgcgtctt tttaacccag gaagctgcat
    12781 ggcaaactgc gccaccccgc cgacgacaat gccgatggcc accgcaagga tcggctcgga
    12841 taaccacggc gataggaaga gcatgcaggc gatcgtaaaa atattgaaaa agaccggaga
    12901 gaaggccggc gctgcaaagt cacgtaacga attcagaatc cccatcgcca gagccgccag
    12961 actgataaag atcaggtagg gaaacatcag ctgcgtcaac agcgtcgtta aggccaactt
    13021 gtcgacgctc tctcgaaacc ccggggccag cagccatacg atgccgggcg cagccagaat
    13081 cccgaccagc gtcacggcgg tcacgatggt gaggagcgtc gtgaaggtcg cactagccaa
    13141 ctcccaggcg tcacgcttgg ctttcaaggt gtgatattcg gtaaagacgg gaatgaaggc
    13201 cgcggacatc gatccttccg cgaacaattc gcgaagcaga ttgggcacgc gataggcgac
    13261 aaaaaacgcg tccgcggccg gcgtggcgcc gaacagcctg gccaacacca tgtcccggac
    13321 aaatccgaga atgcggctgg aaaatgtggc gatgccgatc agacccgccg ccttgacgac
    13381 ggaatgggtt tcatcgacag ctggttgaga tggttcagga gtcgcggcgg gatcggacat
    13441 tggcggaatt gtagcggaat ggagtgaggc cgtctattgt ccgacagaat ccagccgtca
    13501 cagatcttgt atcaccgcat tcggcaactc gttgggatta tcgccctcac gggaaggaca
    13561 ggcccgagcg agaagaactc cggcggcttc gattccatga cagagaccac cagcgaggtc
    13621 accgaccttc atacgctcca ccacgaccga aacaatgtcc tgccactgat ccactgaaat
    13681 gcgcccctgc agtgcctggt ccggcaggat atagacctgt cgctctagca atgagaccat
    13741 cagcaacagc ccggtccgtt cacgggtttg tgcaaggccc tgttgagcaa aggcccgttc
    13801 cgcgcgcatg tgcactttgt gacgcatgcg gtcccgggaa gtgaagagcc gcagaagcat
    13861 gggccaggtt ccaacccaga cgccaacgga ataggcgagc accgtgatgc cgactaacca
    13921 cacggcattc gaagcctgcc atccccaagg caaccaggcg gtttcgatcg tcacaagtac
    13981 cgtcaacgcc agcacggcac tcaataggcc ggcacgatga tgcgcttcgc gatagagccc
    14041 tgatcgcccc acgatcaggg gtacgatctc cgcgctcgtg tgctgctcgg cggcctgtac
    14101 ggccgcctcg acacgcgccc gctcagcttc agtgagtgcc ggcttactac cagtcgccac
    14161 tggcccctcc tcctccgaaa tcaccgccgc cgcccgagaa gccgcccgat cctccgaaag
    14221 aatcgcccag tccgcccgat ccccaccccc cgtgccgtgt gctgaaccag gtgtcatcga
    14281 atggaccgct gcggcttcgc cgtcctcctc cgctcgcttg tcccaatcca ctcatcaaac
    14341 tgaccagcgc cacgcttgcg gcgccggcga taagagccgg gatgacccac ggtgcgacca
    14401 gaaacgagag tccccctcct gcgacgggac ccagtacgcg gtgagcccga gagaagaaga
    14461 gaccaatgac cacgcctaca atgacggcaa caatcgtatt gccgatcacc tcaccatcgg
    14521 aatgaacagc ggtcggcgat tctggtgccc tataggttcc ctcaatagtg tgcaggacag
    14581 cctgtacacc ggccgtgacg ccggccggga catcaccggc gcgaaatttg ggagcgattt
    14641 cattgcggat gatttgcgcc gatcgcgcat ccgtcaagac tccttccagc ccgtatccga
    14701 cttccagacg aattttacgt tctttgatcg caatgaggag cagaacgcca ttgtcggtgc
    14761 ctttggcgcc taacttccag gtcgtcgcga cacggtggga aaaatcgaac agcggctcgt
    14821 cctccaatga aggaacaatc agtactgcga cctgattgga ggtctttgat tcatgcgcct
    14881 ggagggtctc ggacaattga gccgtggtgg cggggggcag gacatgcgcg aggtcgacca
    14941 cgcggccgct gagcggaggt acatcgagag cacgggccgg aaggctcggc accagcgtga
    15001 accatagcca tccggcaagg acagcccacc ctgctcggcc catcagccgc atcgccattg
    15061 ctcctgtcac aggaagccga cgctagaact tcacttccgg tggcttggcc acagcctttt
    15121 catcggccac ggtgaagttg gctttttcgt ccaaatggag aagaaatttg gccgtcagat
    15181 tcgtgggaaa gtagcggacc atcttattaa actccgccac cttgtcgatg tatcgcttgc
    15241 gcgcgacggt gatgcgattt tcggttcctt cgagttgact ttgaaggtca cggaatcctt
    15301 gatctgcctt gagattggga taattctcgg caagtgcgag caggcgcccc agggcactcg
    15361 ataaactctg ttgcgcttct tgaaacctct tgaaggcggc ttcgtctttg agtaactccg
    15421 gcgtgatctg cacgctggtc gccttggcgc gcgcctgcac cacgccttcc aacgtatcct
    15481 tttcatgcgc cgcatagccc ttgaccgtgt tgaccaggtt cggaatgaga tcggcccgac
    15541 gttggtactg gttgatcact tcgctccacg tggctttcgt gtcctcatcg agcccttgga
    15601 gatcgttgta gccgcatccg gacaaggcca ggagaccggc gagtaaaaaa cttgcgccca
    15661 tcaatcgtgc cattgagatc atcgctcctc ctccgcgccg aacggtgaca gcgcttcttc
    15721 aagatctaag gcttttgtcg acgaagtcaa tcatccagtc tgccgcccac cgcttgagat
    15781 gtcgcttcgc tcacaacctt gttatacttc gcagcgggta aggttattgg gtcgcaacgg
    15841 aatcttatgg cgctcgatcg ttctaccgat actccgggag gctttcaggt tcggcaccgt
    15901 gccttgggaa tcttccaagg cagctcgatc gggttggcct tctggcaccc ttcgtcccat
    15961 atgccggaat atggtctctg ccgctttgct acccgggcta aagcccagga ctatgtcgat
    16021 ttcctgtcgt cacctgcgtg cccggaacca ttgagtcagg cagacctcat catagaagat
    16081 ttcgatcacg cggaacacga acgtctcacg acggaatacc cacaggcctc tgcctgggaa
    16141 actccactct agcagaatgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 tctcctgaag tttcgtggga ttcgcgaggc gatgctgcgt agcggagagt cgttaggcag
    16321 gagaacgagg tccgaccgag tccgtcttgg actgtacgaa cgccagtagg cgcttatttt
    16381 cttgggctga ccgcagaaat ttgaaggcga taccgcgaga actcaccgat ctgaccatgg
    16441 cagcgacttc gatgggagcc tgttcattct ccagcacgat ttgcaggtaa aagatatcgt
    16501 cctcatgcac cgatgcctga ctttcaatga tgcaaccgcc cattgaaata tcccgcaccg
    16561 tcccctcccc ccgcaccttt cccccggaaa acgacaaaaa caggcggaca ggaatacgca
    16621 ggtgctcgcg acgatcaatc attcgagccg ggtaccgata gccgaagcgg gacgccgaga
    16681 aacgaaagct gcatgactgg caatgaaacg gtgacatgca ggcgagcgac tgcaaccgct
    16741 ccatgagagt cgagcagggg gaacggaaaa ctttatcctg gccgcactgc gggcaagtca
    16801 acggttgtgc catatccaat aatcggcgcc tctcagggcc tgtctccttc acacgcgcgg
    16861 gatagggctc gcgcgacgat ctatacatcc tgtgcggacg tcatccagca cattccggtg
    16921 ccgatgtggc ctgcaataca tttttcggaa agctcgcgtc tgtcgccagt tcaatcgata
    16981 aaggatggtc ggagttctgt caagaaactc aacggttctg cgcgtagacc atgaggtaac
    17041 tgagtgggct caatctgaag gagggaagaa ttcgacttct tgtcgcaagc gtcgctccat
    17101 ttcgactctc atttcacgtc agattttata cagaatgttc ctcgctgctt ctcaatttgt
    17161 ctaagcagga caagtgaagc tcctgatatc gcggggaaat tttgattaat tcattttaac
    17221 gtcgcactaa cacctcttta atctcccact gttagtctct ccctcacatg ggcacggcct
    17281 tcacgcgttg gaggcacatc ctaaggaggt ggacaccatg acatgcggac gatgcaaagg
    17341 actaatggtt gatgaatggc gacccgattt ttcgccggaa acgtttgtct ggcggtgtat
    17401 taattgcgga tcgatcgtgg atccgttgat cgaacagaat cgacgtactc ggatggccga
    17461 gcaattattg ttggaaccgg tcgaggtatt gcagtagatc gaaccctcag ggcaggggca
    17521 ctgggtgctc ctgccctgta gcacgcgagg tagagtacgg tggcattaca tatcgaagtc
    17581 ggcccaaagc acggtgtgat cggaagcatc ggtggcacgg cgaggctcta aatcaacatc
    17641 gacgcgctga catttctggg ccaacggcgc tgtggccaga atatggtcga tccgccaccc
    17701 tttattggca gctaaggcac ttggcgcacg gtagtcaaaa aacgtgaact gctggcgatc
    17761 tggatacagt ttgcagaaca catcttcgaa gccccacgca atcgtcttcc cataggcttt
    17821 gcgcgcgtct tcgtggtagc agacatgttt cagatgtttc tccgggctgt ggacatcaat
    17881 gggtcttggg gccacattca tgtctccaca ccagatcgcc ggctccttcg gggagagatg
    17941 catatcaaag tgcttcagta agcggtcgta ccaacccagt ttatattgat atttcgggga
    18001 atcgatctca aacccctgcg gcacataggt gttgatgatt ggaatcccct gaatgacgac
    18061 ccgcagcagc ctggcatcct ccgcttctcc gccatcgtca aatccgtaac agacagattc
    18121 aggcttcgtc cgactcagta cggcgacgcc attgtaggcc ttcatacccc ggaacgtgat
    18181 ctcatatccc gacgtcgcga gcgcggcaag aggaaattcg ctgtcttgca ctttggtttc
    18241 ctgcaggcag agcacgtcag gctgatggcg ctccagccac tgcaagacga tcggaagacg
    18301 tttccgcagt gagttgacgt tgaaggtggc gactttcatc gtgggcgcga attctaccag
    18361 agcccaccac gcgcaacaac cgaggtctgc aaaatactac ggcctgtgat cgggaggttc
    18421 cgatcacagg ccgcaggcga gaaaacgcgc cgttttccgt ctacccgtta atagcgggtc
    18481 gcttccttca ggtcgttctt catgccgccc atctcttgaa cggtatcgtc cgcagcactt
    18541 ttcatgtcgg ccgctttcgc ccgggccgca tctttcttcg ctttcacttt cgctttgacg
    18601 tgttccttct tggccttcgc atcggctttg gcttgtttct ttttggcttt tgcttcgtcc
    18661 cgcttcgcct tcagctcgct ctttgcagac tctttcttcg ccttcagatc gctcttcagt
    18721 tcttctttat gggcattcat ctcgtcggaa aaggtgccca tatcctgctt catcccctcg
    18781 gacatttttc ccatgcccag atcgtcggca gcggcgaatg agctcatgcc cacaaatgca
    18841 gcagcgacca tcacagcgat tgaccttctc atcaacaacc cccctcttgg ttgaatatgg
    18901 tgtcaggcat cccggcaatc cgacgggcct gcagcatcca tcaatacccc gaagagtttc
    18961 gtttgccaat ttttttgccg ttttttacgg caaggaggag ggtcgcttac agtcgagagc
    19021 gcggccatcc gcaagagaga cggatcactt gaactacaag cggccttact gacgaatagt
    19081 cgtgaggtag cgccggagaa tggcctgctc cacccgcgat cggccgcggt ccggtgcagg
    19141 aaatcgcagg accagatgaa tccgccccgc ctcgggaagc tggatcgtga tgcgtggctc
    19201 aggcgaaggg gcttccaata aatttcgttg ttccagcaac ttcatttgcc gacccatttg
    19261 atccataaaa ggggcgcact ctacctttgc cgcgccaagt agggcttgtt cggcggcctg
    19321 ccaattgtca gtgcttaaga gcggaaccga caagacatac aaaccatact cttgcgacgg
    19381 actctcctta atgagcggat tgccgaaaag cagactgttg ggaaagacga cggttcgccc
    19441 cgtgtacaag tgtgaggtct gaccagggcc gatctccaga agcttcgtgg caaatacatc
    19501 atattcgagg acgacgccac ggtacgtacc gagttgaatc cggtctccga ctccatagac
    19561 cccgccgccg acgcgtaatg ccgcgccgct ccaacagaga atcagctcct ttgttgccag
    19621 aactaaggta gccgccaatg cgacgagcga gaccgcgaag gcttccaact cgtgcgccca
    19681 aatgaccacg agtcccacaa agaggcacag caccatgcta ttgcgggtgg tcacgatcca
    19741 ccggcgccgc gcgtcgatgg ttagcgcttg gttattttta atccatcgaa cgagaaccgt
    19801 tcgcgcaatg aggaggataa ggaggagaag gatggacttg agcagatcga agaagacact
    19861 ggagccgatc accggcagca tatcttgcat ggttttttcc gtcattgtca ctgcactacg
    19921 gtaccatcct cactcgtgcg tggcacgcat tcttctcttt tccaatcttt caggcgtttt
    19981 tgctcgtcaa atgacaaggt atagcggtag caatacagcg gctcccgata gggttcatcg
    20041 ttaccagcca gactttttcc aaagggtgac ggcgaaggcg gggctttggt ttttttgccg
    20101 gcggccaggc tgctgggatc ccagggatga acttcccggt ctcccatcgg tacgcggtag
    20161 gtccaaacgg tatcgccgcc tagtaccggc gtcttggccg tgctcggtgg gccgaacttg
    20221 tcttcgatat cggcctgggt gagcttgtgc acacccttat tcaggtaggt gtcccgccag
    20281 ggtccgccgc agcctgacaa catcgccagg cacaacagca tgcctaacct gctccatcga
    20341 accgacaacg gcagcaggcg attgctcacg taacttaagc ggttatcgtt ccgagtcacg
    20401 ttgttcccgc acccagcgaa tcgaacaata catcgcatac ccttgagcca tcatcccggt
    20461 gaccagcaag cccaatgcgg tttgcagcca catggtgttg ggaaactcca acccgtagat
    20521 gccaagcatg aagcctccgg caatgaagac tatgcccatt ccgcgcgcaa tcaatgcatt
    20581 ggtccgcaaa tcagacatcg ttcgcctcgt ttctcaccaa cctgttacgc tacaccggca
    20641 catcaggtcg gttcaactct tcccttcatc tacgacttcg ttttcgagcg aatcgcagcg
    20701 gctctcccct cgagcgcgtc tgcttcagga gtgcgaccga tctttcgtaa cgctcccgca
    20761 taatcgatca cactcttggc cacatccgga tggtcaggcc ctaattgttt ttccctgatc
    20821 gccaacgccc tcgcgaagag cgccgccgcc tgtgctgtat cgccttgagc ggcgtacatg
    20881 gcggctaaat tgctcactgc caaggccacc tcgagatgat ccggaccgag aagtttctct
    20941 ttgatcgcga gtgcccgggt caacagccgc tgtgcgtccg cgaattgtcc gtgcttgcga
    21001 tgcagtaccc cgagattgtt caacatggcc gcgacatcca ggtggttctc gccgtggacc
    21061 tcctggtaaa tcttgagcgc ttccaaatag acgggttcgg cctctacaaa tttcccttgc
    21121 gtgctataca cctgagccag atgcgccaag gtcacggcga cgcgccggtc atggggaccg
    21181 aactcctccg ctttcttcaa cgctgcagca taaatctgtt cggcttctcc caattgtcct
    21241 cgctgcacgg ccacttcacc ggcctgcatg gccgcctccc acgtctgttg cccacaagac
    21301 caggccatga acagcaagag cgcgcacaaa ccgacgagag caggccgcct catatccgcc
    21361 ccatctgctc gaggatggat tcagctggat aggtcacaat ctctgccagc gtcggatgat
    21421 agtgcgggat gcgcaacaag tcctgcgccg tcccgtgaaa atacatgacc gcgatgagtt
    21481 cgtgaatgag ttccgccgct tcaggcccca cgatctgcgc gccgatcagt tcgccattgt
    21541 ccgggcgaca caacaatttc acgaatcctg cgagagctcc gagacacatc gccttcccat
    21601 ggtcggcaaa cgggtacgaa gccgtcagat agggaatcgc ctgttcacgg cactggcgct
    21661 cactgaggcc ggcaatagcc acttgcggat ccgtaaacac catttcgcta tctaaccgat
    21721 ggtcgatgac ccgcgctggc acatccggat gagtcgcgtt gaaggcagcc gtttctcctt
    21781 gctggatcgc caaatggacg atcggcgtga gatcgttcac atccccaacg gcaaaaatat
    21841 gcggttgcga ggtccgcata tcagccccga cgacgaggcg accatcgatg accttcacgc
    21901 cagccgcctc tagattgagt cccttcaggt taggacaacg gcccagagca tgaaagatga
    21961 cctcagccga ccggctgtgc gagacaccat tctgccggaa atgcacggtt ttcccggttg
    22021 gggatgaggt cacctggtcg aaggcgaccc ctgtgagtac ttcgatgcct tcgtcctcaa
    22081 aagcagaggc caacgcctga ccgacatcct ggtccatatc ggacaacagc gtcgcaccac
    22141 gctggagcat ggtgaccttc gtgccgatcc gggaaaaaaa ttgcgcgaac tcgaccgcca
    22201 ccaggccgcc gcccaatacc gcgagcgaga ccggtgcatg acgcacatcg agcatttcgt
    22261 cgcttgtcac gtaccccgcc tcagacaatc cggggatggc caccttcgcc acactggatc
    22321 cggtggcaat cacaaacgcg tcacctcgaa tgcgcaaccc tccggcacaa atctcatgag
    22381 gagagacgaa ttcggcgtgc gattcgtaca gcgtgaaccg cggatcgcgc aacgcggcga
    22441 tgcgataatc ggcaaactcc tgcaccagcc ggttcttccg gtcgacgatg gccgagaggt
    22501 cagcccgggc ggtgacaggc gccaacccga actcccgcgc gcgtttcatg agcgccatga
    22561 tttcggctga ccggagaatg gtcttcgtgg gcatgcagcc acgcaaaata cacaagcctc
    22621 ccaacggccc ctgatcgacg atcgccacat cggctccggc ttcgcacgcc gtccgcgcag
    22681 cggcataacc cgccgacccg ccgccgatga ccacgacatc gtgccgacgc tcgttcttct
    22741 gagagagttg tgatggcatg cctgattcga tagactcgct cgcaggttcg aaaccgcaag
    22801 gatacaccat gatcaaaccc ggtcgctatc gtcactataa agggaacgac tatgaagtgc
    22861 tcggggtggc ccggcattca gaaaccgagg aggaatacgt cgtctatcgt caactctatg
    22921 gaacaggcgg cttgtggatc agaccgatga ccatgttttc ggaatcggtc tccatcggcg
    22981 gcgagatcgt gcccagattc cggcggttag aggaaggtcc agcgtgagaa cgtcgccgcc
    23041 tgcccccggc gataacccgc ccgatcgacc acattccaca cgagggcctg ctcgatcagg
    23101 aaccgccgcc cgctgctcga tattctgact ccccggtaat tgtcgatgta tccctgggtg
    23161 cgggcgcgtt caagcatcga ttcacgctcc gtacggttca ccggttccgc cgtcagacgg
    23221 gatggcatat gcaccagctg ctcccaggtc atctcccaga gcgtgagcgc gagttgcgat
    23281 ccgtaattca ggatcggatc gtcttgcatc ccatgtgaga ccaccacgat cggggcagtg
    23341 aacagcgcct gagcctgagc ttcagcccca tccacccgct ccatcaactc tcgaccgaca
    23401 caatggcgat agctgtcgag cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nncgacctga taggtcagat ccagcgggac gccctcgagt atccgatccg ggctgtcgag
    23581 catcggggta cgcgagtgag ctgacttggc tccctgctcg cgtgaatact ggaagacttg
    23641 atagggaaac agataggggg cctgcggatc tttgaagcga taggcagaat cgaatgccac
    23701 caggctccag gtaccgagca gatcctcacc ggaacaggat cgcagcgacg aggccggaac
    23761 ggtcagcccg gatcgcgtca gtgactttgc atgggcgcga tcactcgcca gggccgatcc
    23821 cgacgccagc atgaccgagc agcacgccac cagaagcgcc cactgccatc gcgccatgtg
    23881 catctccatc tcagacggga ttaaggatct ttgcgtgaat ctgccgcggg agagggagca
    23941 ggtaaggcct tgggagctcg cggcaggacg cgatactcga tcagtctgca cgtgcagaac
    24001 gagacctgtc gccattcagc ggtaatcttc acgatcgccg aaccgcagat ggagcacccc
    24061 agggcccggg gatcgcgctt gatcgtccag cgagggatgc tgatgtggtc cgcgttgata
    24121 ggttcctgtt ccatggaaac cttatcggtg cgcggttttg aaatctgaag aggcattttt
    24181 ttccggagcc cctttgccct tgatgcgatg ggcctggtcg catatataat agctcctcga
    24241 tgagcaccgc gccctcaatc tctcgcaggg gcgcgggttt ataaggagca tggccatggc
    24301 tgcacagaca ctgttcgaaa aaatttggaa cgaccacgtg gttcgggcgg aaccggatgg
    24361 aaccacgttg ctctatatcg accgccattt ggtgcacgaa gttacctcgc cgcaggcctt
    24421 cgaagggctg acggtcgcgg gtcgcactcc acgccgatcc gccgccgcac tggcggttcc
    24481 cgaccacaat gtcccgacga ccgaccgccg cgtcgccatt gccgatccgg tgagcgccaa
    24541 acaaattcaa acgctggatg ataactgcaa gacgtttggc atcaccctct tcggcatgaa
    24601 cgacatccgg caaggcgtgg tgcacgtcat cgggccggag caaggattca ccctgccggg
    24661 catgacgatc gtgtgcggcg attcccacac ctcgactcac ggcgcgttcg gtgcactcgc
    24721 cttcgggatc ggcaccagcg aagtcgagca tgtgttggca acgcaatgtc ttgtgcagaa
    24781 gcgtccgaaa acgatggaag ttcgtgtcga cggccaactc tcgccgcgtt gttccgcgaa
    24841 agacattatc ctcgccatca tcggcaagat cggaactgcc ggcggaacag gttatgtcat
    24901 cgaatatacg ggttcggcca ttcgcgcgct cagcatggaa gggcgtatga cgctctgcaa
    24961 catgtcgatc gagggtggag cacgcgcggg catggtcgcc ccggacgaga ccacattcaa
    25021 ctacatcaaa ggccgcccga tggcgccgaa gggcgccctg tgggaccagg ccgtcgcagc
    25081 ctggcggaac ctccacaccg atgccggcgc gaagtacgat gccgtcgtgg aattacgtgc
    25141 cgagacgatt gcgccgcagg tgacgtgggg aaccagcccg ggcatggtga ccggcgtgga
    25201 tggcactgtg ccggaccccc gcagcatgag cgatgacaaa ctccgtcagg ctacggaacg
    25261 cgccatcgaa tatatggctc tgaaaccagg catgccgatc cgggatatca agatcgacaa
    25321 ggtcttcatc ggatcctgca cgaattcccg aatcgaagac ttacgcctgg ccgcctcctt
    25381 cgccaagggc aagaaggtcg ccggcagcgt gcacgccatg gtcgtccccg gctccggcct
    25441 cgtgaagcaa caggcggaac aggagggctt agaccgcatt ttcaaggaat ctggcttcga
    25501 atggcgcgag gcgggctgca gcatgtgcct ggccatgaat gccgatgtgt tacagccggg
    25561 cgaacgctgc gcgtcgacca gcaaccggaa cttcgaaggc cgtcagggcg ccgggggacg
    25621 cacccatctg gtgtcgccgg ccatggcggt ggcagcggcg attgaaggac atttcgtcga
    25681 tattcgtcag tggtcataag caggaagaca attcgttcct gacgccgggt ccgctgatca
    25741 cagcggccgg cagacacgac gcacaaaagg acataccatg gacgcattca ccacactcac
    25801 cggcctggtc gcgccactgg accggctcaa tgtcgatacc gaccaaatta ttccgaagca
    25861 gttcctgaaa accatcaagc gcaccggtct tcgggaaggg ttgttttatg attggcggcg
    25921 acgaaaagac ggatcccagg atcctgaatt tttcctgaac cagccgcgct atcagcaggc
    25981 ctcgatcctc ctgacgaggg acaacttcgg ctgcggatcc tctcgcgaac atgccccctg
    26041 ggcgctgctc gaccagggat tccgatgtat tctcgccccc agcttcgccg acattttcta
    26101 caacaattgc ttccagaacg gcattctgcc ggtcgtcctg aaggctgcag aaattcaagc
    26161 gctgttcgag ggcgtggctg cacatgatgg ctacacgttg acggtcgact tggcggctca
    26221 gcaggtcaac acacctaagg gcatggccta tcacttcgac atcgatccat tccgaaagga
    26281 ctgtctgtat cgcggattgg acgcaatcgg cctgacgctc cagcacgcag cgcaaatcgg
    26341 cgaatatgag caacgccgcc gcactcaagc accctggctg tttcaagacg tgaggtaacc
    26401 gtcagaaaag gagatctatg acgtggtggg aaacccttct gtttcctgta gcctggctct
    26461 ggagacccaa acttctcttc acgttgttgt ttttcggcgg tgcagcctat ttgctgatcg
    26521 tctttaattg gagctactcg gacggcgacc gtgccgggta cctgcaaaaa ctgtctcgaa
    26581 aaggatggtt ttgcaagacg caggagggtg agctggcgat gacgaccgtg ccaggcgtcg
    26641 ccccggtgct gtggagcttc acggtgtggg acgagaacgt ggccaagaaa ctcgacgggc
    26701 agatggggaa gcgggtcgtc ctgcactaca aagagtttcg ctatatcccc acgacctgtt
    26761 tcggtgaaac cccctacttc gtggatcgtg tcgaggttct ggactgatct cctgattcag
    26821 agccacttct tttgcttgaa gaagtacagc atcgccagtc cggtcaacgc catgacgccc
    26881 agtaccgccg gatatcccca gggcgatttc aactccggca tgtgctcgaa attcatccca
    26941 tagatgctgg cgatgaaact gagcggcatg aaaatcgtgg tgatgatggt cagcaccttc
    27001 atcacgccgt tcaagcgata actcacgctg gacagataca cttccatcat cgacgagacc
    27061 atttcgcgca gggtttcgat cgtgtcgcca atttgaatga tgtggtcata cacatcacgg
    27121 aaaaatactt tggtggaccc ctggagaaat tgtccatccg accgcgacag gttgttcatc
    27181 acgtcccgga gaggccagac ggagcggcgg aaaaagagca gctgccgctt gagtgcatga
    27241 atgtcccgca gtgtctcagg accgggattc gtcacacaga gatcctgtaa cgcttcgatc
    27301 ttttctccca gtgtttccag aatgacgaag tatcggtcga cgatcgaatc catcaaggca
    27361 tagagcaggt aatccgcgcc ggcatggcgc aaacggcctt tcccattgcg tagccgttcc
    27421 ttcacgcctt gaaagacgtc cccgccgttc tcctgaaacg agagcacaaa attctctccg
    27481 aacaccaggc tgacctgctc aacattgatg tcgccctcgc tttggccttc gtagagcatc
    27541 ttcagcacca gatagccgta cgtgccgtaa tcgtcgagct tcggtcgttg gtcggtgttg
    27601 gcgatatcct ccagcaatag cgggtggagg ccgaacattt tcccgaaggc ttccaacacc
    27661 tccatcttat ggatgccgcc gacatccacc catcgaacgg tcggctcccc cgtcgccacc
    27721 acctcgtccg gcttggacac cgtgcgttcc tggtactggc cttcgccgta ttcgtagacg
    27781 gtaatggtga ccgtctccga cctcttttcc ccgatatgga ccagtgtgcc gggcgggagg
    27841 cctgccttac gtgaatgttt gtgtttcaac ttcatcgtac tgccagcttg tacgcggaac
    27901 ctgcaagagg gtcaagagcc gcagcatccg catgaagcga cgtcgggctt tgttcccgtc
    27961 ccaagacctg gtaggttgaa gagaagaaga ctcgcccatt cccgggagcc atgactatgg
    28021 agtacgaact gaccatcctg tcgcaagcca tgagtgaagc cggccgtgaa gcgcttcgct
    28081 tagccagcgc aggatttgaa acgtttacga aagccgatca gtcaccggtg acctctgctg
    28141 acctggctgt gaatcgcatt ctccaggaca gattgtcgac agcctttccc gaggacggct
    28201 ggctttccga agagacaacg gacaacgaca cacgcttgag tagaaagcgt gtctggatcg
    28261 tcgatccgat cgatgggacg cgttcgttcg tgcggggcct gccggaattc tgcctgtcgg
    28321 tcgcgctggt cgaaaacgga gtgccgaccg tggcagccat ttttaacccc gcgaccggcg
    28381 agtttttttc agccattcga gggcgtggcc tccgtgtcga acgactgtcg gattctgatc
    28441 aaccgggctt cacctcatcc gaacggccgg tcgcgctcgt caatccatgg gaactccggg
    28501 tcggccggtt cgaaggcctg caggaccatc tccactgccg cccaatcgga tccatcgcct
    28561 acgcgcttgc gctcgtggca gccggtcaag cggatgcggc gatcaccctg gccggcggaa
    28621 atgaatggga cgtggctgcg ggagttctgt tggtagaaga aagcggcgga cgcgccacga
    28681 ccgccgctgg tcatcccgtc accttcaatc gcccagaccc gcgcttaccg ggaacgctgg
    28741 ccgtcgggtc ggacctcccg gcgccggtgc gcacccgcct gattcaacat ggcctcgcgg
    28801 ccgcatccca tccgctttga gccttttcaa cgcgcgcgcg tctctgtacg atggccgcat
    28861 tggctacatc cgagaggtat tccatgaacg tcgccttgtt aggcacagga ttgttgggcc
    28921 agggcgtagc ggaacggctg catgccaccg gccacgacct gacggtctat aaccgttcca
    28981 tcaaaaagac tcaggcactc cggcagcaag gcatccgtat cgctctgact gccgcagagg
    29041 ccatgtcggc agcggacgtc gtgctcctcc tgctgtcgga tgccgcagcc atccgtgcgg
    29101 tgttgttcga tcccgcggcc agtcgcgcga ttgacgggcg cacgatcatt caaatgggca
    29161 cgatcggacc ggccgaaagt tgctcactcg agaaagacgt cacaggtctc ggcggccggt
    29221 atcttgaagc gccggtgctc ggcagcattg cggaagccaa agccggcacg ctgctggtca
    29281 tggttggtgc gaccccggag cactatgaca agtgggctcc gcttctgcgt tatctcgggg
    29341 aagaggtgcg cctgatcggg ccggtcggaa aagcggccgt actgaagctg gccctcaatc
    29401 agctcatcgc cgctgaaatg gcggcgttcg cattcagtct gggcctcatt cgccgaggcg
    29461 gcgtcgatgt gaatgatttt atgggagtgg tgcgaaagag cgcgctgtac gcgccgatgt
    29521 tcgagaagaa actatcccgg ttggagaaac ggaactatgc tcaaccgaac ttttccacgc
    29581 ggcatctcct caaagatgtg gagctggccc tgtccgctgc ggaaaaggcc cgcctttcgc
    29641 cggccggcct tcagggagtc cggtcactcc tcatcgacac gattgccgca ggccttggag
    29701 acgtggacta ttcggcgatc tatgaacaga tcgatcccct cggctccgag cacgggtagg
    29761 aatcagcgcg cggaaggcca aggctgattg tccacaatcg attgtacctt catcacatga
    29821 aatcgcccgc cggccttggt gggagctttg tcaccttcaa tgcgcgtgta ccaccaccgc
    29881 ccctcgcctt cggaatagtc gggggacagg gtaatctcaa agcccccttc ccgatcattt
    29941 tctcgttgag cccaggctcc agcccatcgg tggtcggaaa acagatccgt ccggaatcgt
    30001 ccgtctttaa aatcgtagga cccgtttccg aatcgatcca atctcaacgg caccaccatc
    30061 ccgccttctt catattccca ttcacccgcg agaactgcgt gctccccgcg aggcaaggaa
    30121 atttccccgg cgggcggttc ggtccacgtg gtttcttgag gccgtggcgc atgactacag
    30181 gcctggatgc ttaacaggct cagtccgatc acgaggaaaa accattgttt ctgtttcatt
    30241 ctattcatcc cgggtgtttc cttccccctg tatgcatcag ggtaatactg tacataccat
    30301 gcgtatccca tgtgctcaag gctgcgatca aattttgctc atttccacca ctctggcttg
    30361 atcactccag accaagagcg atctcgccac acggcggaat gtcccttcta agtaattgag
    30421 aaatctctca tcaactcaat atctcaaggt cacgcggtcc caaccgataa agaatcggca
    30481 ggaaagatct gcgccgatat tccgacacgc caggattttg cacgaggcag gagttcctct
    30541 cgcaacccgg cgaaggctgc cacatccaat tgggagagac ctgttgatga tgccatcttc
    30601 agacaataaa cacgcagggg gcgaggctca gaatccctct cactcgtctc ctctgaacat
    30661 cctgctcgtg gaggatatgg aagataatcg cgtcctggtt tccctcttac tgaaggcgtt
    30721 tccgtatcgc ctggatctgg ccgaacacgg tgcggcaggc gtcgagaagt ttcaatccgg
    30781 ccggtacgac ctcgtcctca tggatattca gatgccggtc atggatggct acgccgcgat
    30841 tcgcctcatg aggcaatggg aaaaagagca gcagcggagc gagacgccca ttctcgccct
    30901 gaccggcaac acccatgacg aagacatcgg gaaggcccag gccgccggat ttaccgccca
    30961 tgtgatgaaa ccgctgaaga aggtcacgct gctggaagcc attcaacgct atgcggctcc
    31021 cttctcacgc aaggagtctg ccgcatgagc gacatggcac ctgtcacgtc cagctgcggt
    31081 gccggaccga tcgtggtcct ggtcgactct gcgtttgaac cgctgattcc gaaattcatg
    31141 gccaaccgga acaaagaact cgcgacgatg acggaggccc ttgcgacaag cgactttgcg
    31201 accatcaggc aactggctca tggcatgaag ggtgtcagcg gcagttatgg gtttcacgcc
    31261 atgacgacca tcgccggtcg aatggaggag gcggccaagg tcgccaacgg aacatccatt
    31321 cgccaggact tgctcgaact ggcctcgtac ctcaccagag ttgagatcac gtacgaatga
    31381 cgaggcccca tgtgctcacg tatctggtcg gtggcaggct caatgactct acgctgccca
    31441 tgcagcacgg tggccgggcc ggggagtgag aaaatcaaga agtatgcatg tgtttgtaag
    31501 aatccgtgct acaaaagaat gaactcgtcc ggatcaggac aatctgaata ttcaggcccc
    31561 tcactcatcg atacctaagg agcgatgtat ggataacccc gaaacaccca aactcagggt
    31621 tctcatggtt gaagatgagg aagacacggc gagcctcttg aagtttctgc tggagcgggc
    31681 caactatcag gtcgttcatg ccaaagacgg ccggcaggcg caggagctcg tcaataccat
    31741 cgcgcctccg gacatcgtgc tgttggacgt gatgctgcct ttcttaagcg gattgcaggt
    31801 actcaccgtc atcagaaagc gcgagggctg gaaaaaagtc ccgatcgtca tgttgacggc
    31861 cgacgggagc gagcatgata tcaagcgcgc cttggagagc ggtgcaaatg attatatgat
    31921 caagccgttc aacccgcgcg aactgacgag tcggctgaag cgctttgtcg cacatgcggc
    31981 ctgagcgagc gccgagagga atggcgtgct gggcctcgac cagtcagacc tgacgctccg
    32041 catcggcacc atcgcggcca tttccatcta ttcctccgta ttgcttctcc tccttggcat
    32101 tctcctgctt cgatacatac gcaacaacaa agaccggcgt cgacgcgccg tcacggaaat
    32161 ctggcggccg atactggcag aatgtgtggt cgaagtgcct gagacgcttc ctccactcac
    32221 gacccgcgac cacctcgtcc tgctctatct ctggaatcat tgctacgagt ctattcgtgg
    32281 agacgcccgg gaaaatctga aagaacttgc ccggcgaacg ggcactgatc ggttcgcgaa
    32341 ggaactcctc catgcccggc tgctccggcg ccggttgctc gcgatcgtga cgctcggcca
    32401 ccttcaagaa cgatccatct ggaacgagct cgccgccgcg ctccaggccg ataacgcgtt
    32461 cgtctccctc gtcgcagcca aagcgctgct gcatatcgat gcgaaagccg ccgtccccct
    32521 gatcattccg gtgatcagcc agcgaaagga ttggtctccg ctcaaagtcg tggcgatctt
    32581 cgaaccggca ggtgcggaca tcgccgctga agccatagcg aaggctgcgt gccttgccag
    32641 cccggaaatc gggtcgcggt tgattcgcca cctggcagcc actcaaagcc agcatgcctt
    32701 gccgccgtta cgggacatgc tgcagaaagg gacgccgtcc gatgatctac tggcagcctg
    32761 cctgttcctg ttcggagaat gcagtgatcc tcgagatatc gtgacgatcc ggacgcacct
    32821 ctcgcatcca acctggtatg tgcgactcca agcggcgtcg gcattgggga aggtggggat
    32881 tgaggaagat gaagcacgac tcatcgccct gttggatgat gagcattggt gggtgcgcta
    32941 ccgggccgct gaagcgttgt cgaatctccc ctggatgacc acggagcgtc tgacccgcgt
    33001 gcacaacaca ctcaccaccg tcgagtcaca ggaacacttg gttcctttta tggctcaatc
    33061 gaatgcgaag gctcagcatc cggctcgcat cgcctagcgc atgaccgcgc acaccgctcc
    33121 ttcactacag gcccatgttc actgtgttcg aacgcgatga cgacgtgcga gaagccgacg
    33181 gggtcaaaga actgggcgtg aagaggacgg tgtaggtgcc ctgcaattga gggaatgtga
    33241 ccatcgttcc atgttgcgcc actccccgca gcgggttgcc tgagcgtgcc acctcccatc
    33301 cgggcggcac ctcgagcgtc aagcccgcca aagagactgg aacgttcaac gtcacccgca
    33361 tggcatcggc atcgcgcacg acatcgacct ccacccgatt acgcgccgcc caccatcgtc
    33421 cgaattctcc caccgagccg aaccacgcgt aggttcgcac gccctccacg aaccctttct
    33481 caaaggcgag tttgtgatcc agcacattcg gatgaatgag cacgacgaca gagcctccgt
    33541 accggctgat acgtttcgcg acctccaggg cctgcggaag acggtctccc atcttcggta
    33601 acgcttcatc ttcgatcgtc acgggaaact caaaaatctc gacctcgctg tcggtcagac
    33661 gatcgtaatt cagttggtat ggcaaatggg tcagcgagtt attcgccgtc gccgtggaac
    33721 tgtaccgata gccggtcgcc aggagcgctt gcggaagtcc aaagggattg gacaattcgc
    33781 ctggcctgaa agaaacgatg gattggccgg aaaagtgatc gatgagaaac ttgctcactc
    33841 gcaattcgcc caggatgctc acattgtagg ccgtcgtccg atccttcacg aacggggcat
    33901 acgatggata ggattcgcgg ccgcttccca tgggaaactg attgaacacg cgtgaatggg
    33961 cgatcgtatg gctacccaac tccatgccga gatccgctaa ctgcttcaca taccggacgc
    34021 cctcgtcgtt aaagaagata tcgtcgttat aatcgcgaat gtatttcacc tgaatgaaat
    34081 acgtgccgac gacgccctgg cgtttctcat actcagcata ctcaaccgcg tgtttgatgg
    34141 actgtgtaaa atccacatcg tgcgtcatca tgaccgacag cgacttgccg aaaggcacgg
    34201 ttcccagggt cacggcctct tcctgcgccg tgcgatacat cgctttgatg agccgcaacc
    34261 agacatcgag cgtcgggtcg aactcattgt cgaatgtctt gaccagctcc tccccgcgat
    34321 tgttgtatcc cttcagcagg aggaagccga gatccactcc aatcgcatag gctcgaccgg
    34381 gcccatgagc cacctgcgta acggcggcgg tcccatcatc gaaacgcgca agcggagccg
    34441 acgcgtcggt gtaaccatag gttcccatca cctcgatacc cttgtcacgg atgccgaccc
    34501 gcagccgttg ctcacgcggg tcggtcagat ccgccaacag aggatcggcc gtagccaacg
    34561 tcatgtcata gcgctgacgc gaggcaacag cgtcacgaaa cccgaagacg tgattcaatc
    34621 cgccacccag cacttgcacc ccgatcagtg tgccgcctgt cttcggaaat tccgccagcg
    34681 cttgtagttc ctccggtttc aacaccttcc cggatatcat gggatagacc agcaccacct
    34741 tatgcgccaa ggcctcatga acattgcgcg taatacgaaa gggaatcccg atggatttca
    34801 gcccgtgggc taagccgagc cacgatgagt ccggatcggt taagagaatg gccaaccggc
    34861 ttgtcgaacc cggttcgtag gtcgtccacg cggtcggcac gtggcgcggc acgaccgtcg
    34921 cgtcgtcggg gcccgccacc ccctggaaac gatgaaagtc tttctccgga acctccatgt
    34981 gctggacggg aggagcactg acgagaatcc ggacgccaat gacgagagac acaaagagca
    35041 gagcaatacc tgacacacgg acgagcgtgg accgttgcat agacctacca atgatagatg
    35101 aggttgaagt tcccgccccg tcggacatag aggaaccccc ggtcttcgta gtatccggac
    35161 gtttcggcgg agaatcgttc attaatcggg aacgtgacgc cgccttgaat cgtgcgactt
    35221 ccgacgcgcg taaagtcttg tgccgctacg atgcgttcgc ctgacgtgcc gaacgagccg
    35281 gaggcgaaga attgcaaccg atcggccggc cgccaggtga gctgagaggc cagtgtgacg
    35341 gcccccgtat tcggaataaa gtaaaccttc tccgtcagcc acagattgaa gggaagaaaa
    35401 atcgtcaggc ccggcatcaa tagatcgatg tcatcgtgct tgtaattcaa acgccggtaa
    35461 ccaaacgacg cttcaaacgg tgccagtgag gagtgcagcc cgcccaggcc ttgcgcgact
    35521 tcgccgacaa aggaatagtt gggcgagaaa tgcggattga tcgtggcctg ccccgcgatg
    35581 tacccccagg cccgcgacca caagggacta tagagctcgg ctgacattgg cgtgtcgtga
    35641 aatccaaagc gattgatcgg ttccactcgc atcaccagcg tttgattacc gagcggtttg
    35701 gcgagttcaa acaagaggtc gcgttcatcc ggctgattct tcgtataggt atagtggccg
    35761 tagccgatct tggcgtagtc ccggtaggga agacgcaggc cggccagccc ctgtccgacg
    35821 gccgcgcgcg gcgacacgag caattctgat ttcaccgcct tgatctgctg ctcaatcgcc
    35881 gggtcatgcg tgtcgtccag cagggcctca taccgctgca acgcttcgga tcgatcgcca
    35941 cgccaatggg ctacctcggc cagtccgcgc cgagcctcga tccgagtggg ctcggcctgc
    36001 gagactgccc cgtacaatcg ttccgcctca tcaaattgtt tctgccatga caagacttgc
    36061 gccagcgcga ggcgaatgtc ctgatcctca gggtgccgag ccagcacttc acgatagagg
    36121 gtcgtcgctt ccgcgtgagc gccctgccat gaaagcaccc gcgccagcgc cgtgcggatg
    36181 tcgtcattgt cagggtgccg gcgcaggaca tcgcgataga cgcgttccgc ttcaggatat
    36241 tgtttggcca cttcgaattt cgtggcttct tcaacggcgg ccccgatcac tcgctcgtcc
    36301 gtgccctggg agtcgtgagt tgacgcaggg tgctcggcaa cctgtggcga ggtcgtgcgc
    36361 cattccgagc gaatagtctt cagctgggct tcgagctcgg gatcgtgtgt ttcggcaacc
    36421 aacgcttcat aacgttgaac cgcttctgcg taatgtccct gccaatgagc cacatcggcg
    36481 aggccgcgcc tcgcctcgac atgcgtcggg tctgattgga gcacgtcctg gtatacgcga
    36541 ccggcttcct cgaattgctt ctgccacgag aggacttgtc ccagtgccac acgaatatcc
    36601 tgatcctggg gatggcgcgc caggatctct cgatataacg tcgtggcttc cgggtgaacc
    36661 ccttgccagg acaggacccg tgccaacgcg gcgcgcacct cgtcattcgc cggcgacagt
    36721 tgcagttcct cgcgatagac ggtcaccgca tcggcgtatt gacgcatcgt ttccagacgt
    36781 ccgccccgct ccaatgtttg agccgcgtga gcggtcaggg aatcgttccc atcagcagcg
    36841 ccggacggat tgacagggac taccggtgaa cgagaagcgg ccttcaagga ttgaaggcgc
    36901 gcggccacgt ccgcatcgcc ggtcaatgcg accactcgtt catagtacgg aatggcctga
    36961 tcctgatgac cgctccacaa gtacacattg cccaggccgg tgagggcctc gacatggtcc
    37021 ggttcgtcgt ggagcacccg ctcgtactcc tctcgggcga gatcatagtg tttctgccac
    37081 gagagaactt gggcgagacc gacccggatg tcgtgatcga gggggtgcct ggtcaggagg
    37141 tcgcgatagg cggccgcagc cgcgtcccat tggccctgcc aggacagaag tttggcgacc
    37201 gacgcacgga catcgtcatc gtcaggtttg gcctggaggt attgccggta cgccgcaatg
    37261 gcttcctcgt accgcttctc gtcctccaag actctggctt gttgcagagt ctggcgggga
    37321 gaaagctccg gcgccatggc ggcaatcatc gtcccgggtg caaagaacca cgccgcggca
    37381 aacgcaatca ccgcgagcag ccctgctacg gacgttcgtt gattgatcca ggtgaaggag
    37441 tcgttcatcc gtgaagactc tgtgcttcca acaggcacgc cctattcggg gagggcccgg
    37501 aatccatcga gatcgtgaat aatcacccca gcaaaactcg gatgcatcgt ggtgtcgagc
    37561 atatgactaa tagcctggga cacttccacg cgggaacgtc cggcaaaggt cagacgttcg
    37621 ggcctgaccg taaatcgacg atggatgcgg aaccattcgc cggatcccgt cagccggtcg
    37681 gcctcggaga tcggcttcca ccgtagccgt ttgtgctcgc gatctaacaa ggcatcggca
    37741 tggtccggac ggagatcacg ccgcagcacg acatgctctt caagcggcaa caccgttgtc
    37801 tcaacggcca gccataccgg cttctcactc acatcgccat accgcaggat gtcctcggcg
    37861 atgtcctgca cttcctcgag atcggtacga taactcatga ccgccacttc atcagcccga
    37921 tccatcacgg cataggcgag cggtcgacca cccacggtcg gggtagccag ccagaaggga
    37981 atcaccatcg acaatttggc gcggccgtgg atggcctcgc tcaccgtctc gatcgcgccg
    38041 agataccgac ggagctgccc gtcatcatcg aggaatccag gcaagagata cggctcgata
    38101 tcgagctgta tgccggatac cgcaccaccc cgcaccaggt gaaacagtct ctgtacctta
    38161 tcggccaaaa ggtgaggttc ctgaatggct tcggggtacc catccagcgc cagtgcatca
    38221 atcccggcct cctgcacctg agccatcaac gacgcgtagc cttcccagat cgcctcatca
    38281 tcgttgagtg atggcatttg gatgagcatt ctcgaacagg cttgcctgcg gcaggtagcc
    38341 agcagtggct gtggctcctg tatcgccgca cggtagttcc acacccagaa accagtccca
    38401 ccgggtggct tcttcaccgt attacgttgc gacaccgtca gctgctcgaa ttcgatccgg
    38461 ccgcgtggtc cttcagcgga cacgacgagt gccgtcacat atcgaaggtc cactccgcga
    38521 ccaatctctt tcaggggaat agtgagatca aatgtccccg tcactgttgc cagcgactgg
    38581 ttgtgctccc gctgatgacc ggccagatct tctatggcta ccaccacctg gccggtggct
    38641 cgcccacgaa gccgcagcat ctggtaggac atgagatcca tcggccttcc ggcgggacgc
    38701 agctcgatac gctgaccaca acgtcccgcg tccccggcag tccactccaa ttcgagatgc
    38761 gagtcgcttc gtaccgcctg tccatggcag ggaccaaaga catgggccac cccgaatccc
    38821 ggtaacagtt caagaatggg taaaaacggt cgataaggaa gaggacccgc aggaggcact
    38881 ggcccgctga atgccacggc tggatcggtg ctgttcccgg cataggtcag ttcgatctca
    38941 tggatcgtgg aaggaatcgg cggggacagg ggaatgtcct ccgcctgcgc aagagaaagc
    39001 cacaggatgg agatgacggc agcactgcat aacacggtcg cgaagaggcg ccgcccgacg
    39061 gcctctgtgc cccgaccgac ggatttcccg tcaatctggt ctcgagcgac accatccctc
    39121 gcggagacgc attgatcgat gtgggtagac acgctcattt caacttctga gtggctgttc
    39181 ctgctctact ttctcggcct gaccgccgga tacttaatgc tggatctcat cgcgatcgtt
    39241 cacctgcggc aatacctgca ggagcgtgtg ctggaaagcc tgccgcacag ttacaccggt
    39301 ttcgaaccgc agatcagcat cctggtcccc gcctataacg aggccgccac catcgccggc
    39361 tctattcggt cgctgctgca actcagctat tcggaatatg aaatcgtggt gatcaacgat
    39421 ggatcaaagg atgccacgat cgatgtcctc cgcaaagaat ttgatctgtt tccgttcccc
    39481 gaagcctgta atacgcaact gcctacggcg gaaatcagag gaatttatca ctcctcgagt
    39541 cataccaatc ttcgcgtcat cgacaaagaa aacggcggca aggcggactc cctgaacgcc
    39601 ggcatcaacc tgtcgatcta tcccctgttc tgctgcatcg atgcagattc cattctccag
    39661 ccggacagcc tgcggcgagc ggtgcaaccg tttctcgacg accatcgcac ggtcgccagc
    39721 ggagggacgg tgcgcatcgc gaacggttgc cgcgttgaaa acggcttcct gaccaagatt
    39781 ggattgccct ccaatctgct ggcactgttc caaatcgtgg aatacctccg tgcctttctc
    39841 ttcggacgac tgggctggtc gccgctgaat gccatgctga ttatctccgg cgcattcggc
    39901 attttccgga aagaggccgt cgtcaccgta ggaggctatc gatcggatac gatcggggaa
    39961 gacatggaac tggtcgtgcg cctgcatcgc catctgcgga tgggcgacaa accgtatcgc
    40021 atcaccttcg tgcccgattc cgtctgttgg accgaggcgc cggaggactt gcgtacgctc
    40081 aagaatcagc gcatccgctg gcaacgcggg ctgtgcgaaa gcctgacaaa aaacctcgat
    40141 ctcctgtgcc atccgaaggg cggcacgatc ggctggctgg cgtttccgtt catggccgtg
    40201 ttcgagtgga tcggcccggc cattgaggtg ttcggctact gcttcctgat cttcagtttt
    40261 gtcatgggat tcgtctcgat ccaggcgtgg ctgatcctgc tcgtggcggc catcggcctg
    40321 ggcgtgttgt tgtcggtgag tgcgctttta ctggaagaaa tgtcgttcca cgtctacccg
    40381 gatcagcgcg acgtgttgaa actgttgctg gcagccatcc tggagaattt tggattccgc
    40441 cagttgaatt ccctgtggaa gttaatcggc ttgtatcgat gggtctgcgg gaagaaagca
    40501 acctggggaa ccatgacgag gactgcgtcc tggcaggcgc ggtaaggatg ctggtgctgt
    40561 cactgagttt atgaggtcaa tc
//
LOCUS       DAOQEW010000012        36819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_224_length_36819_cov_9.875802, whole genome shotgun sequence.
ACCESSION   DAOQEW010000012 DAOQEW010000000
VERSION     DAOQEW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 36819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_36819_cov_9.875802"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="PLS35_02675"
     CDS             <1..2048
                     /locus_tag="PLS35_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI17585.1"
                     /translation="GVELAKTYGSKIVGSHVYAAKMHDKRFKQMEAGLPEEYHDEKEL
                     DRQRQIHDSLITRGLQIITDSYLDYVDKKCNEANLPVERRSLEGRNWKVLAEDINTNA
                     YDLVIMGALGVGAVKDSVIGSNTERVLRRVRNSDMLIIKQPTPVGTGKIVVAVDGSPY
                     SFGGLMTGLALGKAFNVPVEVISAFDPYFHYAAFHSISGVLNEEAGKVFRFKEQEKLH
                     EEVIDSGLAKIYQSHLDISREIAQAEQTDVKTTLLDGKAFEKIIQYVRKENPWLLIVG
                     RIGVHSDEDMDIGSNTENLLRSASCNILVSNRKYVPPIDTQAEYTIAWTEEALRRMER
                     IPVFARGVAKTAIHRYAIEKGHTIISNTVVDSAVGHILPKGAMDAMRALGGNLDAAGI
                     DRDKMQADDSVAQDLMGSTLSGMMTQVVEEKPAHSPGTQAYLDRMSQNYFVCDGCGYI
                     GKGDTPVKCPVCSAEGDRFKQVDKSIFEAAAKAEGGLETDVAYDDIPMQWTKDAKEAI
                     RAVPAGFQRRRAKAKIEKSARKLGMTTITLEYANPMIQEAASEDYTPIFANKGTGTAG
                     QPSAEVPTANGTESATANGNGTEQPQASQAPSSPYTWTPDAQGRLDRAPEGFMRECTK
                     ALIEKHADKIGTTVITLEVATEGIEQAKGYMADAMKTGNLKDMIANLTGSSKTGANR"
     gene            2048..3451
                     /locus_tag="PLS35_02680"
     CDS             2048..3451
                     /locus_tag="PLS35_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI17586.1"
                     /translation="MGKSLPVLNIPFLGDKLESLQQQISKFISPTSPGTAPNDGRTVD
                     DFKPYLVALNLTKRCNLKCDHCYLDATTKAGGGSDELSTEECFRLIDQIAEVNKGCLL
                     VITGGEPLVRPDILDIARHAVGLGFIVVFGTNGMLINDRMAKTLVEIGVMGVGISIDS
                     LNPEKHDHFRGVPGAFASALAGIEACKRNGLQFQVHFSAQPMNYQELPAVVEWAHTLG
                     ARVLNVFFMVCTGRGEELTDITPQQYEEVLGYLVDCQDQYKDMLVRARCAPHFKRLAY
                     EKDPNSPLTKATGYMGGGCLAGTNYARVTPNGELTPCPYMPLSAGNVRETSFADLWER
                     SDVFNSFRYPQLKGKCGDCEYTDICGGCRARPYVDHGDWLDEDQWCLYTPKGGDKIKV
                     AFNTPEESEVAWDEASELRLSRIPYFLRAMVKKGVERHARENGIALVTIELMEELRKK
                     RFGNDAPVFNFDMRGKD"
     gene            3518..4033
                     /locus_tag="PLS35_02685"
     CDS             3518..4033
                     /locus_tag="PLS35_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17587.1"
                     /translation="MDSVQSIFLDLNQLIPIVNHWFHLLSAVIWIGGLAFIVMAVKPG
                     LAEAALPKEYVRPITDAFYKHYKRVAGVLLVVLLFTGGINLHYVNQIFVSQTGQGMQH
                     HPKYLWIFCIKLILVLCLLTLFLYTVIFKTDQDEEEEGENYESIPFQRAALWMGFFII
                     LCAAAMKHLHQ"
     gene            4354..6132
                     /locus_tag="PLS35_02690"
     CDS             4354..6132
                     /locus_tag="PLS35_02690"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HNI17588.1"
                     /translation="MRTRLYRSFALTALLLTLSPLHCRAIDPSPNQISAESLNQSLSD
                     QDLQQWYHLSAGTQLIPYDWLVALLDESLAKTFAVTGLVEDPRHPDKLPVGFAKTEGP
                     NVPVPLVGFTCSFCHTTQFRYKGHSIRVEGGPSLQNNAALVNALIQRLGALTPPDVSA
                     FLQSLKEEAPPEPFKTFANRVLASRGEAVTAYTLTKLAQDVRVLTQALVSRSGKDISP
                     QQWGPGRFDALGRGGNTVFAPLNPDNLRPANAPVSIPALWGVWEFDWVQWAGSIQHPL
                     ARNIGQVIGVNAGLFSWARPGVPTPSDKNKVFHSSIDVTSLTILENLARRLPPPQWPS
                     VFPPINRELAARGKDLYHGNKAKGIPNLCAHCHVPSKVSHSSANSPSLQITMVPLQEI
                     GTDSLYLENFSQRTVDTSFLGRGRISAREASEYVTTELMTLDKTGDLSSYRDRPNIWR
                     DKAQYIARPHFGIWATAPYLHNGSIPTLYELLSPARERRACFVLDPNMEFDPEKVGFV
                     TGDCANPSQSPTLPPRLEFRTALPGNSNRGHEFANTPDCDSAKSNGVLGCELPVNDRL
                     AVIEYLKTCDLDRLILPNAPACPNFD"
     gene            6201..8918
                     /locus_tag="PLS35_02695"
     CDS             6201..8918
                     /locus_tag="PLS35_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI17589.1"
                     /translation="MTDLLSSDHSILERPFMEFRPFGLNDKGEKISDISGVVVQSNVD
                     YMCDYLSRTAGSQAAAAAVDELCRLLNARLPDRTYHVTPDFLRNIWNSYSYEFVCYLR
                     EFCEQLSGDPQFHINVGTDRKVPPLIQILCRPFSTQQLYKMWPYFGRKYVRDVLEFEV
                     GRVTRRSAVLRMTFTERALVQFGPYRKRCVDVICLSCKSSIARVQTQLHGTPAATVKD
                     LSCTAHGDAYCEWEFTWTPQVRSSIPVAMWTLVAGGTFTYLHVRWPDVPTFEALGLAA
                     VPASLLWWMITSHMRQATKPLQRLIKDQEQVVDARHEELREAYLEQQSTAVMLRRKVN
                     DLTTLHRAGLLFSSTFDREELLTDVLDTIVRDLHYDRAMITQFDRTRQVSHDFRVRGM
                     PQEVADFLRTQEVSVTDPETVEGAVFLRGEPVLTNNVHEIWDRLHPFDQKLIAMVNVK
                     SFITVPLKTHDAVIGSLSVDRTHNQALTQDDLDMLVTLASQVAIALDNAQAYREIEAL
                     NAGLEVRVRERTAELEAANTQLKQMDRLKSKFLAHVSHELRTPLTSIVGFADNMLEGL
                     VGSLNMKQEQYLTRIRANGTRLARMITDLLDLSRVEAGKLVLSFDHVALQTVVSDVIE
                     QLLPLAIAKHLQIELRSPDPALLVWADPDRVSQILTNLLDNAIKYTPDGGHITVDLSV
                     GDHEMARVVVRDTGQGIPADALPKLFDPFFRVHHQERSQTKGLGLGLAIVKDLVDLHG
                     GSIMVHSTVDEGTEFTFTLPLDSRAPEPAAQVPAARRRLLVVDDDADICDLLRDRLES
                     EGFHVDTAADGRTALRILAETPVDGVLLDIALPEVDGFEVLRQLRPQQPALPVVMMTA
                     VEALDRAMAAVEAGAQGYLLKPFDAARLRQIVDHWFLAGASKTDPTSGR"
     gene            8923..10311
                     /locus_tag="PLS35_02700"
     CDS             8923..10311
                     /locus_tag="PLS35_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNI17590.1"
                     /translation="MLFSRRFRIALVLSLVLLPVSLSLSVTPVQAQTPRLQGQSASAA
                     AMGNAFVAQADDPSALHYNPAGMTQLHGFQTLFGTSLIGGTTQFTGPSGAQATGDRNG
                     SLAYPPPGHVYLVANLKDLGFAALGDFSAGIGLNNPFGSLTRYPNDGPFRSALTFTTL
                     PLLDIKPTIAYKLNDQLSFGLGADIYTFSGLFGEGHLEQKSVWPGGLGIPAGSLVEIN
                     GSDTTAGFNVSLLYTPYRNSEGKPLVNIGMVYRSQATFHLTGNFLANGTSIAGTAATF
                     VLPQVFSGGIAVWPVRTPDREWKLEFDVDYVGWKSNRTLDIHLTNGTVLPFPQNWQSG
                     YTAMVGTEYRWLRLASLPDWDVALRAGYMNQQTQVPDRTFNPGVPSANTHIPSVGIGF
                     ACHEHGYFLGLIRCGELGIGPLKPKLFAIDLSYQAAFYEVRTVTGNQNPTVNGRYDTL
                     IHAGSLSLRFNY"
     gene            11309..14806
                     /locus_tag="PLS35_02705"
     CDS             11309..14806
                     /locus_tag="PLS35_02705"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNI17591.1"
                     /translation="MPGLCQAQPSSPITSSGLNTTVTTSGNSFNITGGTRPGGGTNLF
                     HSFGEFGVPANQVANFLNDSGLATTNILSRVTGGTPSNIFGTIQTTGFGNANLFMMNP
                     AGMVFGPNASLNVGGSVHFTTADYLRLGDGARFNAMPGPQDAAVSSAPVAAFGFLGSN
                     PSAIVVQGSQLTLAEGNTLSLIGGNVQIANGTLSDGAIQPARLSAPGGQINLVAVGSP
                     GEALISNGAGTLAEPMLTGFTGLGTISLSHGTTIGTSGDAAGRIVIRGGHFSMEDAGL
                     EAISTSVSPNSLPSSSPRGDISIQAEEVSLSNGAHLLTSTINGTAGNIALEVGTLRSN
                     VGPGGLPLSGAAPVTITSSSTGLGGPGSISVTGNGRAASDVVVLSNTQAVTTVPHSAQ
                     PTIVPAHIDMTAEHLELRNGTVLKTDTTGGADAGSITLKVGTLATEAGPDGRVLLSSS
                     SNCGTGCVGGQAGDITIQGIPGITPAETHLYVPIVRPDSPPTVAISYQLARNMDLRGT
                     DIRSEAIGNAPGGQVIMRAQGSISLADTTISVATQDFQIDGPKPSGELVRNQGFSRID
                     ILAHDVTLKDSTVRADANVSDIGSCPLCLGGPSAGEIWFRVGNSFTADNSAIINSGKG
                     RAQAGLTKIIRDHFFSFGAIWEPDYPDYPTNSITLNHSQITVESPHSGIPGYLRLRAD
                     NVTLNHSILNSQVNDVSNSRGLDGQLIDVPGAGERGRVIADGRDVQGSVLVSAKTLDI
                     TGGGIIAPTTGSRIASRIELLANELTTHRGTGPGGTFDAPRILDAADPTRVVLSSSST
                     GSGGAGRISIAGEGVAMPEGTPYRPASSIHLDGTALFTDTHSDALGGKIEMISRGPIV
                     LHDTTVSANVTDVRPASAGLQEQGGNISLSAGSLLVQGGGLSALSRGTQTGGNIAITV
                     QGPITLESGAAISASNTGSANAGSVTIHAGSEFVSRDASVTTESIHASGGNIFIQATD
                     SIRLVNSRISTSVLGGPNSSGGNIILDPAVVTLQNSQVLAQAVQGAGGNIRIIAGTFL
                     ADQTSAVSASSEFGLNGTVSIQSPVSSLSGTLATLPQSTLQTQQLVTQRCAAQGSGHL
                     SSLVVAGRDVLPVEPGGWLMSPLSVFAVDAHDDGGRPVAETIPDAAEGGNTLVELTQG
                     HPTPEPRSNFTTPGGGCRS"
     gene            14907..16604
                     /locus_tag="PLS35_02710"
     CDS             14907..16604
                     /locus_tag="PLS35_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNI17592.1"
                     /translation="MTFQALAQVAPPLPPIFDPTGKSGKPPALLKEDFNTPPSEPPSS
                     ILPKVPTTPPALPDNKLGAFRVFVHDIHVTGSTVFSETELSEVTAPYRNRYLTTEDLE
                     KVRLALTLLYVNNGYLTSGAVVPDQDVTFGTIVIQMVEGKLAHIDVEGNAWFRNAYLR
                     DRVARGVSTPLNIHPLQERIQLLQQDPRIERINAELKPGDVRGESVLNVRVKDANPFR
                     AWLEFNNHQTPTVGAERGLATVAHQNVTGHGDQFMFTYGRSHGVNPIIDTSYSLPLNR
                     YETTLTAYYRRNAFLVVENPFRALDLNVDSQVIGLALRQPVYRTVTAEFALTLTGEHL
                     FLKTTSAFDAPGLPSLFIPGSSTTGVATVSALRFSQEYTHRSSSLVFAALSRVSVGLN
                     VLGATINSGPLPDGQFVSWLGQAQVVKRLDDWWGVQLLGRVAAQLASDRLFPLEQMPV
                     GGRFSVRGYRENTLVRDDAVLASFESRVPLLRFPSGEDRLQFAPFVDVGHAWNAKGHT
                     PDPHTLASVGAGLRWMILPQERARFEVYWGQQLNHVRGGEGNLQDHGIHLQLVVQVL"
     gene            16605..18947
                     /locus_tag="PLS35_02715"
     CDS             16605..18947
                     /locus_tag="PLS35_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNI17593.1"
                     /translation="MRHHRLTVFPPPSHLLRYGLKGLSLCLIIFFCCTVSLAADSQPS
                     AGDLMQRGRAAAREGSYEEALLAWKAAAELYDQAGETKNHIQALTQSAFAARTLGHIN
                     QAFLQEELALHLARQLRDSQWLVITLSELGKTYVTNHQYEVAADYIGQALEIAQAQSF
                     RALSPALQNDLGIVLALQGRPEEALAAFHQCAVSAKQEGLIPLFIRARVNAARMAIQV
                     AQFDTATSALAEAADQLAGSASSYEKAEGLVNLALGYRDLALAHSGSHASALKRSALF
                     LQEAAGMTQHPGDARLASYVYGHLGHLYEVERRFDEALQLTRQAVFTAQSARAPESLY
                     RWQWQSGRLLNRLGQLDDALSAYQEAAITLHPIRSEVTFAVQTSPDSHQPSIRALYFE
                     LADLLLRRAALMHENREAEAYLRAARDVIEAFKAAELRDYFRDDCVDQMQARITKLDA
                     LSPSTAIIYPIVFSDRTELLVSLPDGLTRHSVPVTAATLTDEVRRFRHTLEKRTTREY
                     LPHAQQLYDWLIRPLDPDLRRLKVNTLVFVPDGSLRTIPMSALHDGKEFLIEHYAVAM
                     SPGLDLTDPQPIDRAKVHLLSSGLTKAVQGFPPLPHVADELDSIRALFKGEQLIDQEF
                     LTPRLEEELKGGRYSILHIATHGQFAPDVNQSFLLTFDARLSMTQLERLVGLFRFRRE
                     PLELLTLSACQTGSGDDRAALGLAGIAVKAGARSALATLWSINDDASSELVSEFYRQL
                     HDASVSKARALQHAQLKLLSDRVYEHPAYWAAFLLLNNWL"
     gene            18996..19703
                     /locus_tag="PLS35_02720"
     CDS             18996..19703
                     /locus_tag="PLS35_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNI17594.1"
                     /translation="MARTFVATLLFTVSLVSTNAAAPEGVPDVPQYNAPQKMTPRARL
                     GGSLRGSEGEDPAVAALVPDHVGITVKQHPALNWFLSKHTSLPITFTLIDERSIHALV
                     ERPLAPPHQAGVHAVKLEDLGVTLQPNIQYRWYISVIKDADSPSQDIVTGGMIERCEF
                     SECSILGAMTTCTREAVMTSAAKGFWYDAMGCLCDLIDSNPADTALRKQRAALLRQVG
                     LHDVADWDLAQSHARQG"
     gene            19707..20447
                     /locus_tag="PLS35_02725"
     CDS             19707..20447
                     /locus_tag="PLS35_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HNI17595.1"
                     /translation="MPYRRVSFPLVLMIAWLTGGYASQFPFGAPDSIAADPLVPLELR
                     LPEYKPKKADAPRARIGGHSRGTEAGAPVLIALVPDHIAFTVKNDPTLCWYQSLHTSR
                     PMVLTVVDSRGIRPVLEHSLPTPVRHGIQCLRPREYGVEFRVEESYRWYVTVVMDPNR
                     PSLDVVAGGMIERLSLDEACALGMPCPSSSCDTDGIYRYAESGLWYDAIACLVELLEQ
                     ESDKDRLRLMLDHLLHQSGVHLPGESSP"
     gene            20830..20955
                     /locus_tag="PLS35_02730"
     CDS             20830..20955
                     /locus_tag="PLS35_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17596.1"
                     /translation="MELVMGGLLVGVSGMLAVLIVAVWKDSASHRTGAHPSHRRV"
     gene            21060..21341
                     /locus_tag="PLS35_02735"
     CDS             21060..21341
                     /locus_tag="PLS35_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17597.1"
                     /translation="MNRFVYLRPQKRQRMTVSLPADLLERMRDAAYWTSGTTMAGLIS
                     SAIEDLLHNLESQNGRPFSPRLQDLKPGRPRSNKPDQSSVSENIQSVSR"
     gene            21561..23651
                     /locus_tag="PLS35_02740"
     CDS             21561..23651
                     /locus_tag="PLS35_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI17598.1"
                     /translation="MRTRTARAETIVHCPFTATQPEPALAVHPITLRWDHYTRALARG
                     LMSVCWAFAIEAHSPIPTYAAPQADAPANEELELLKEEETVSIATRHEQPISQAPSNV
                     YVITDEDIRHSGATDIPTILRRVPGLDVMQVTGADFNVSARGDNQLFANKILVMVDGR
                     SIYIDGQGLVFWKSLPVTLPEIKRIEVLKGPASVVYGFNAFDGVINIITKSAQEMKGT
                     TLQFGGGELGTISSAAVQAGSAGKLDYRLSIGHDQNQQWRNNDALAFRSNKFNAQTQY
                     NFSGDAILRVAGGFIDTNRFDGPVSNVQVPRSQFNQGYSDVHYQYRNFSLRGWWQGND
                     IPVESSTHPLLAPFVRQTNATGGSNVSFTTNTYNIEAQHSLDFGPANRLSYGVNYRYN
                     SVDGAAVSELGREDRLGFHLQDEWKILSSLTLVAGVRYDLHTRINGTWSPRVALIYNL
                     TPEHTFRVSGSVAYRPPTLFESNLDLRILPTVPPLFAPIPLFGSQRLNPEQIISYEAG
                     YQGWWLKHRLRTRLDLFFNHLSDLINFQTIGATRTLVNGGEADIYGTEAGFEFLATRW
                     LSGFANVSYQKIGQTFTGVSRRGGPDWKANVGLRGDWDNGLNGEIAVHYVAGATYPLI
                     ELFTALAPVLPAPISPNIDSYTLLNLRGGDRFWHEKAEVAIAVYNALNDRHKEHPLGD
                     TLGSRVMGWLTLRY"
     gene            23655..25721
                     /locus_tag="PLS35_02745"
     CDS             23655..25721
                     /locus_tag="PLS35_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI17599.1"
                     /translation="MHAESKGVWQTMVAAFMTVSISQFAAAEPPAIRVEDSLTVPANN
                     DLELLKEEETVSIASRYEQPISQAPSNVYVVTDEDIRQSGATDLPTVLRRIPGLEVMQ
                     MTGADFNVSARGDNQPFANKMLVMVDGRSIYLDVQGNVFWKSLPVTLPEIKRIEVLKG
                     PASAMYGFNAFDGVINIITKSPEEMKGTTLQFGGGEQGTISSAAIQAGTVGKFGYRLS
                     IGRDQTQQWRDGDALAFRAHKFNVQTDYALSSTSKLQLSGGLIETNRYDGQVGEVTSN
                     SIRPSFGYANAVYEHGAFLVRAWWSGYTDHATINPLPQLAGLLSVTDRNGVSTNPFSG
                     NTYNVDVQHALDMGAANRLLYGANYRHNSLSSPAIDQFSRENRLGLFLQDEWRASSAI
                     TVVAGIRYDLHSQINGTWSPRLTLLYQPVEGHTFHLSGSAAYRPPTLFESHQDQRVTT
                     NIPTGLPFPLPSSVSSTVPVTGSTGLAPERIISYEAGYQGWYWKHRIRVRADLFYNHV
                     SDLIGSRITASGSSEFVNDPGSADIYGGEAGIEVLANRWLSGFANYAYEEIGQSFTGT
                     VRRGAPRSKVSAGVRTEWDNGLSGELSYYYVGAATYPLAQSFTQLGTIPGAGITVPGN
                     QVGSYNLLNLRAGYRFWQQKAAAGYMRDAEVAISVFNALNDEHKEHPLGDLIGRRVMG
                     WLTLRF"
     gene            25752..28874
                     /locus_tag="PLS35_02750"
     CDS             25752..28874
                     /locus_tag="PLS35_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17600.1"
                     /translation="MAPATLTHSILVVDDDPDIALGLQDFLDHDGYQVNVAMTCAEAL
                     AQAHAHHYNAVLLDLGLPDGDGSSVLLTLHQQQPQLPVIILTAYTSTDRTVGSLTQGA
                     FAYLTKPYNRDELRAVLQRAVGVQALAVKAEHAEHALHESEARFRSLVEAATDSIVLA
                     DHNGHILSWNRAASHLFGYTDEEVRGQSLSILMPPRHRAAHERGLARVRDTGESRLIG
                     RLVELEGLRKDGSEFPLELSLAMWKSDEAVFYSGIIRDITQRRRAEEILDRLRHLHEV
                     ILTQAGEGIYGLDRDGITTFVNPTAAELLGYEPFELIDRPMHGLLHHSRSDGSPYPSG
                     ECPIYAALHDGLVHRVSADVFWRKDGHPVPVEYVSTPIIEKGRTAGAVVVFRDISERK
                     EAERAVEESQERFRQLAEHIREVFWITNPEKSHVVYISPGYEEIWGRSCDSLYAHPTS
                     WLDAIHPEDRPRIEEAAISRQALDVYNETYRILRPDGSMRWIWDRAYPIRDASNQVYR
                     IVGFAEDITEQKRIEAELIASERRYRVLFDDNPSMYFMVDAVGTVLSVNRFGAERLGY
                     EVQDLVGTSVLNVFHPEDQQAVLHNLSDCLSEVGQPKRWELRKVRKDGHVIWVRETAQ
                     AVRGHEQAPVVLIVCEDITAMKEAEIALHDSEELKNQILHSSADCIKVLDLEGRLQYM
                     NDAGQTLLEIKDLGTYTNKPWSEFWEGDDREAALAALEAAKAGDIGKFVGFCPTTTGQ
                     AKWWDVQVTPMLDTQGYSRRLLAISRDITAYKQVQESLHASEERLEHVIRGSHDGFWD
                     GHVLPDHPWYAPITPVWWSPRVREMLGYNEEEFPDILDSWMSRLHPEDREAVFERLNA
                     CMEQGVSQYDVEYRLLNKQHEYQWFHARAEVVQRDAQGQAVRMAGALQDITDRKRTEA
                     RLRLSEERLRMALTSSDLGIWDWDVTSDRLYWSEGVELLFGLAPGSFPGTYTDYIGLI
                     YLLDRGSTLTRLELSLRDQTSVHIRHRVVWPDSSIHWLAWSGRIYRNADGVATRVLGI
                     VHDTTGRMDE"
     gene            29007..29900
                     /locus_tag="PLS35_02755"
     CDS             29007..29900
                     /locus_tag="PLS35_02755"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI17601.1"
                     /translation="MVLLLWTLLIASPALATDVIILKSSDIAAYNQAINGFKAAIPAG
                     INLSEYDLQGDLEKGRKLARKIRASDPSLVFAVGLKAAKAAQLEIVDIPVVYSMVLDP
                     AKYGLNAQNMTGVLLEVPAERQLVMIRNLLPQLKHIGTLYDPAKTSTLIDEARRLLKQ
                     SGTELAPMQISSERDVPGSLRALLPSVDALWLVPDSTVLTDESLRFILNTALEARVPV
                     IGFSREFARSGALLCLSVNYADIGRQAGQLTRKLLEGQLSLPLKPWHPDRIETSLNLK
                     TAKFLGIDIPRELEQKADELY"
     gene            29900..32212
                     /locus_tag="PLS35_02760"
     CDS             29900..32212
                     /locus_tag="PLS35_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI17602.1"
                     /translation="MKGPSVKDPAHLAAPPFLPNGLKRFVSLRTKFVVFFSLIIIFTC
                     SGMSWYFIHSKRVAMTERVQDLGSILVKNLAHNVRYGIILEDRVILEQFIDGVLGVDE
                     VVYAQITGADGQVLAAKSKGKLKTPLGTVRAVDQPFYPSAAIAASLIRHPLPEPTVTR
                     LRLIGESSLPFEEQDATLRVSTAGMREETFYDFALPVLKRAEPRLEALALQEEETHKT
                     RTAASTAPAFGVVQIGLTEVPMQRELAEVLRTFLLLTLGIITAGILSTNLLARRIITP
                     LRNLAAGARKVGEGDLSTFVVPTTQDEVGQLTALFNVMTKSLQERNQAISANLETIRR
                     HVTQLTTLNQSSAAITSTLDLDRLLSTVLQLLSENLGFARMVLFLWDSERGVATVGQM
                     LGMPPEAEEAARRLVMPVHEDGGFAAEILLHGKPVLVPNIETVADRISPAVLPWLRQV
                     GLSSFVAAPLRSQQRILGYLGADRGAQPCTQEDLDLLVTIASHVAVAVDNARAYTELA
                     TLTEHLERRVEERTHELQSVNERLQDHDQRRSKFVSVASHELRTPMTSIKGFVENMLD
                     GLTGQLSERQEHYLQRVKHNVDRLTRIINQLLDWSRLDVGRIDIKPESLSIDAFVRDV
                     VESFQTLAAEKSITLDVLPCEQPLVVYADRDKLEQILWNLVGNAIKFTPHAGHVSVQC
                     TAHDTHSVQITVADTGCGIAPDELPLVFNEFSKVESAMPTSQGAQLGLFITKSLVTLH
                     GGQMWVESRLGAGTQFSFTLPLSATQPAQP"
     gene            32379..35183
                     /locus_tag="PLS35_02765"
     CDS             32379..35183
                     /locus_tag="PLS35_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17603.1"
                     /translation="MAFAAWVLRTSTNTGCFTRQRGPTVHTAVLIVDDDRDIRESLTD
                     MLRHEGYLVESAGSGNEALSHAKQRQYGAAILDIQLPDLNGLSVLKVLMELDASLPVI
                     ILTGNATSENTIGSLAKGAFAYLTKPYNAQELKAVIRRAVSVKGLAVRAEHVEQALHA
                     SEERFRALVESATDAIVLADHYGHIIWWNGAAERMFGYSKREALGQSLAMLMPERFRS
                     AHETGIMRLTTGSAAKLIGKTVELVGLTKAGDEFPIELSLASWRAKEGAFFSGIIRNI
                     TRRKKAEESVVRLSHQNALILDAAGEGIFGLDLEGRTTFVNATAARMLEWSAAELIGK
                     PLAPLLYRLHDEGAEYVQDPLCLSAAVRDGAVHRVQNDVFTSKSGVSFPVQYVTSPIR
                     EQDRPVGVVVVFQDITARKQLECLQQAQLSISHILAQTSTLEEAVPQLLRVIGEMGPW
                     EMTIFWHKEPMNERLVCEGTWMRSSCRWDEFATICRNSEFPPGMDFPGVVWSTKLPLW
                     IPDALTDARFTRAPTAARLGIHGACLIPIRTGTVIHGVLELYADKPRLPDRQLIHILT
                     DTGMKIGQFIDRARAGSALRATHRMTQNLLASLPGAIFLCARDFKIQYANTLAHHYFA
                     VGGASLVGRPLFECLALSEAATQQLIKEWAALNADSPHQAPERECEVASRLYRYRFFP
                     VATPESAQYQVGIVLWDVTDDKHLQDQLIQSEKLTSLGTMVSGMAHEINNPAQAILSM
                     AELIQEEKDPAVIKEFAADIVGYARHVSNVVRDFAGYARSAGRDGETEIDVAERLMEA
                     VKMARRGPHFGYVDVVTRFDAPTFLRARKSELDQVFVNLINNATQAMDGKGRLTLGAA
                     QDGAWIDVTISDTGPGIPAAVRPRIFDPFFTTKAPGKGTGLGLSIVHKIVTRHAGTIT
                     VDSTEGEGTTFRLRFPALLQ"
     gene            complement(35226..35987)
                     /locus_tag="PLS35_02770"
     CDS             complement(35226..35987)
                     /locus_tag="PLS35_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNI17604.1"
                     /translation="MTQACASSVTEAVERAISALDLDRLERDYWEQNEFLYIPQFLPR
                     EFVEAELASQAQVLKSGLNRNYIPGHKKGGSVSYYTVREQAPQFMDLYRSDAFRAMLN
                     QLTRVNLLLCPDNDPHSCALYYYTEAGDHIGYHYDTSYYKGARYTILLGLLDQSKQCR
                     LVCDLFKDVPGKQPVHMELATAPGDLVIFNGDKLWHAVTPLGEQEERIVLTLEYVTNP
                     DMGAFKRLYSNLKDSFAYFGLRSVFKRAYSSRSRA"
     gene            complement(36203..>36819)
                     /locus_tag="PLS35_02775"
     CDS             complement(36203..>36819)
                     /locus_tag="PLS35_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNI17605.1"
                     /translation="EVQEVVKNIQSRYEKTKDLQASFTQKTRIEGFSTPVISTGHFYI
                     KKPGKLRWDYIEPATEEIYVNKDDVKMYVPEHKQVLVGKLTYMAASQAPLQLLQGVAK
                     LDEEFDIEPMPNKERGAGGILLVSLTPKQGRAEPERAIQKIVIEVQPKTYFLKTIALH
                     EVSGNVATFEFSELKPNSGLKDDLFDFKAPADVEIVRAPVLSRP"
BASE COUNT         7925 a        11059 c        10121 g         7714 t
ORIGIN      
        1 tcggagttga gctggcgaaa acctatggct caaagatcgt gggtagccat gtctacgcgg
       61 cgaagatgca cgataagcga tttaagcaga tggaggctgg ccttccggaa gaatatcacg
      121 acgagaaaga actcgaccgg cagcgccaaa tccatgactc gctgatcacc cgcggactgc
      181 agatcatcac ggattcgtac ctcgattacg tcgacaagaa atgtaatgag gccaacctgc
      241 cggtggaacg tcgctcactc gaaggacgca actggaaagt gctggctgag gatatcaata
      301 cgaacgccta cgatctcgtg atcatgggcg cgttgggagt cggagccgtc aaagacagcg
      361 tcatcggcag taataccgag cgagtcctgc ggcgcgtgcg caactccgac atgctgatca
      421 tcaagcagcc gacgcctgtt ggcacgggga aaatcgtcgt cgctgtcgac ggcagcccct
      481 actccttcgg cggactcatg accggtttgg cacttggcaa agccttcaat gtcccggttg
      541 aagtcatttc cgctttcgat ccctacttcc attatgcggc ctttcacagc atctccggcg
      601 tgctcaatga agaagccggc aaggtgttcc gcttcaaaga gcaggaaaag ttgcacgagg
      661 aagtcatcga cagtggtctg gcaaagatct accaatcgca cctcgatatt tcccgcgaaa
      721 ttgcccaagc cgaacagacc gatgtaaaaa cgaccctgct agacggcaag gccttcgaaa
      781 aaatcattca atacgtgcgg aaagagaatc cctggctgct catcgtgggc cgaatcggcg
      841 tccacagcga tgaagacatg gacatcggca gcaacacgga aaacctgctc cgctctgcct
      901 cctgcaatat cctggtttcc aaccggaaat acgtcccgcc gatcgataca caggcggaat
      961 acaccattgc ctggacggaa gaggccttgc gtcggatgga acgcatcccg gtgtttgctc
     1021 gcggagtagc caaaaccgcc attcaccgct acgccattga gaagggacac acgatcatca
     1081 gcaatacggt cgtggattcc gccgtcggac atatccttcc gaagggcgcg atggacgcca
     1141 tgcgtgcgtt gggcgggaac ctggatgcgg ccggcattga tcgcgacaag atgcaagccg
     1201 acgattccgt tgcgcaggat ctgatgggct cgacgctgag cggcatgatg acgcaggtcg
     1261 tggaagaaaa gccggcccat agccctggaa ctcaggcgta tctggatcgc atgagccaga
     1321 attactttgt ctgtgacggc tgcggatata tcggcaaagg cgataccccc gtgaaatgcc
     1381 ccgtgtgcag cgccgaaggg gatcgattca agcaggtcga caagtcgatc ttcgaagcgg
     1441 ccgctaaagc ggagggcggc ctcgagaccg acgtcgccta tgacgacatt ccgatgcagt
     1501 ggacgaagga cgcgaaggaa gcgatccgcg cagtaccggc gggattccaa cgccgccggg
     1561 ctaaagccaa gatcgagaaa agcgcccgca agctcggcat gacgacgatc accctcgaat
     1621 acgctaatcc catgattcaa gaagcggcgt ccgaggacta caccccgatc tttgcgaaca
     1681 agggcaccgg aaccgcaggc caaccttcgg cggaagtacc gaccgccaat ggcactgaat
     1741 ccgctacggc gaacggcaac ggaactgaac aacctcaggc ttctcaagca ccatcttcgc
     1801 cttacacctg gaccccggat gcgcaaggcc gtttagatcg ggcccctgaa ggattcatgc
     1861 gggaatgcac caaggcgttg atcgagaaac atgctgacaa gatcggcacg acggtcatca
     1921 ccctggaagt ggcaaccgag ggaattgagc aagccaaggg ctacatggcc gatgccatga
     1981 aaaccggcaa tctgaaggac atgattgcca acctgacggg ctcgtccaag accggggcga
     2041 accggtgatg ggaaagtcgt tgccggttct caatattccc tttctcggag acaagctgga
     2101 gtcacttcag caacagatct cgaagttcat ctctccgact tcaccgggca cagcgccgaa
     2161 tgatggccgg acggtcgatg acttcaagcc ctatctcgtc gccctgaacc tcaccaagcg
     2221 atgcaatctc aagtgcgacc attgctatct ggacgccacg acgaaagccg gcggcggatc
     2281 ggatgaactc agcacggaag aatgcttccg cctcatcgat cagattgccg aagtaaataa
     2341 gggatgcctg ctggtcatca ccggcggtga accgcttgtc cgtccggata ttctcgacat
     2401 tgcccgccac gcagtagggc tgggctttat cgttgtgttc gggaccaacg ggatgctgat
     2461 caatgatcgc atggccaaga cgctggttga aatcggcgtg atgggcgtgg gcatcagcat
     2521 tgattcgctc aatcctgaga aacacgacca ctttcgcgga gtgcccggcg cctttgccag
     2581 tgcattggcc ggcattgaag cctgcaagcg taatggcctt cagttccagg tgcatttcag
     2641 tgcccagccc atgaattatc aggagctgcc cgcggtagtc gaatgggccc ataccctggg
     2701 cgcacgagtc ctgaacgtct ttttcatggt ctgcaccgga cgcggcgaag agctgaccga
     2761 tatcacgcca cagcagtatg aagaagtgct gggatatctg gtggactgcc aggaccaata
     2821 taaagacatg ctggttcggg cacgttgcgc tccgcatttc aaacggctgg cttacgagaa
     2881 agatcccaat tccccgctaa ccaaggccac cgggtacatg ggcggaggtt gccttgccgg
     2941 taccaactat gcccgtgtca cgccaaacgg agaactgacc ccctgtcctt atatgccgct
     3001 gtcggccggc aatgttcggg agacgagttt tgccgatttg tgggaacggt cggatgtgtt
     3061 taactccttc cgctaccctc aactcaaagg caaatgcggt gactgcgaat acaccgacat
     3121 ctgtggtggt tgtcgggctc gtccttacgt ggatcatggc gactggctgg atgaggatca
     3181 gtggtgcctc tatacaccga aaggcggcga caagatcaaa gtcgccttca acacacctga
     3241 agaaagcgaa gttgcatggg acgaagcctc tgagcttcgg ctcagccgga ttccctattt
     3301 tctccgcgcg atggtgaaaa agggcgtcga acgtcatgct cgtgaaaacg gcatcgccct
     3361 cgtgaccatc gaattgatgg aagagctgcg gaagaagcgt ttcggcaacg atgcgcctgt
     3421 cttcaatttc gatatgagag ggaaggacta ggagccggcg cgagacaggc cttcggtaga
     3481 ctcccctacc tttgctatcc gccctagccc atctatcatg gattccgtac aaagcatctt
     3541 ccttgacctg aaccagctca tccccatcgt gaatcactgg ttccatctcc tctcggcggt
     3601 gatctggatc ggtggactcg ccttcattgt gatggccgtc aagccgggcc tcgctgaagc
     3661 cgccctaccc aaggaatatg tccgccccat caccgatgcc ttttacaagc attacaagcg
     3721 ggtggccgga gtcctgttgg tggtcctcct gtttaccggc ggcatcaacc tccactacgt
     3781 caaccagatc tttgtgtctc agactggtca gggcatgcag caccatccca agtatctctg
     3841 gattttctgc attaagctga ttctggtcct ctgcctcctc acgctgttcc tctataccgt
     3901 gatcttcaaa acggatcagg acgaagaaga ggaaggcgaa aactacgagt cgattccctt
     3961 tcaacgcgcc gccctctgga tgggcttctt catcatcctc tgcgccgccg ccatgaagca
     4021 cctgcaccaa tagtcctttc ctcgaccctc cggacaacga atctcgaccg tcggcatgct
     4081 gcccggccgc cgggccggaa tagtctcaaa ggttcatacc tcataactcg actttcaaaa
     4141 tctattggca atcagagggc gatccaggta cccttcgtcc gcgcctatgg catgtgagtt
     4201 gctttctgct acggagaggg ccgcgagttt catcgctcca ccattgccgc cctcgctcct
     4261 acaaggaaca tacggtcgtc tcggttactt gagcatcgga gtgactatgc ctatcccgac
     4321 tcattgctct cgtccttcat gaggagatga atcatgcgca cccgcctgta ccgctccttc
     4381 gctcttaccg cacttctact gacattgtct ccactgcact gccgggcgat cgacccatct
     4441 cccaatcaga tctctgccga gtcgttaaat cagagcttgt ctgatcagga cctgcagcag
     4501 tggtaccatc tgtcggccgg gacccagctc attccttacg attggctggt agcactactt
     4561 gatgagtcgc tggcaaaaac cttcgcggtg acggggcttg tcgaagatcc tcggcatccg
     4621 gacaaacttc cggtcggctt tgccaaaacg gaagggccga acgtcccggt gcctctggtc
     4681 ggcttcacct gttcgttttg tcacacgacc caatttcgct acaaggggca ctcaatcagg
     4741 gtcgagggtg gcccctcgct ccaaaataat gcggccttgg tgaacgcctt gatccaacgg
     4801 ctcggagccc tgacgcctcc cgatgtgtcc gcatttctcc agagcctgaa agaagaggct
     4861 cctcctgagc cctttaagac ctttgccaat cgcgtgcttg ccagccgcgg agaagccgtg
     4921 acggcttata cgctaacgaa gctcgcccag gatgtgcggg tccttaccca ggcgctcgtg
     4981 tcccgcagtg ggaaagacat ctcgccccag caatgggggc caggtcgatt cgatgcgctg
     5041 ggtcgcggtg ggaataccgt ctttgcaccg ctcaatcccg ataatctcag acctgccaat
     5101 gcccccgtaa gtattccggc attgtgggga gtgtgggagt ttgactgggt gcagtgggcc
     5161 ggttcgattc aacatccgtt ggcgaggaat atcggacagg tgatcggcgt caatgccggg
     5221 ctttttagtt gggcacgtcc cggcgttccc actccctcag acaagaacaa ggtgttccac
     5281 tcatctatcg atgtgacttc gctcaccatc ctcgaaaatc tcgcacgccg cctgccgccg
     5341 ccgcagtggc cgtctgtctt ccctcctatc aatcgggaac tagcagcccg cggcaaagac
     5401 ctgtatcacg gcaacaaggc taagggaatt ccgaacctgt gtgcccattg ccacgtccct
     5461 tcaaaagtgt ctcactcctc ggccaatagc ccaagtctgc agataacaat ggttccccta
     5521 caggaaatcg gcaccgactc cctctatctg gagaattttt ctcagcgaac cgtggacacg
     5581 agcttcttag gacgcggacg catttccgcg cgggaagcct cggaatatgt cacgaccgaa
     5641 ttgatgaccc tggacaagac cggcgaccta agcagctatc gcgaccggcc gaacatctgg
     5701 cgggacaagg cccaatacat cgcccgcccc cactttggca tctgggccac cgccccctac
     5761 cttcataatg gctctatccc gactctctat gaactattat ccccagcacg ggagcgtcgc
     5821 gcttgcttcg tgctcgatcc gaacatggaa tttgatcctg aaaaggtggg ctttgtcaca
     5881 ggagactgtg ccaatccctc tcaatcacca accttgcccc cacgcctcga attccggaca
     5941 gcattaccgg ggaacagcaa ccgtgggcac gagtttgcaa acacgccgga ttgcgacagc
     6001 gcaaagagca atggagtttt gggatgtgag ttgccggtga atgatcgcct ggccgtcatc
     6061 gaatacctca agacctgtga tctcgaccgc ttgattttac cgaacgcgcc ggcctgtccg
     6121 aatttcgact agcagaggac gtgaaaccat ccggtgccgc tcgtacatga cggagatgca
     6181 tcgaaggagg ccaccgacgc atgaccgacc ttctctccag tgaccactcg attctcgagc
     6241 gtccgttcat ggagtttcgc cctttcggct tgaacgacaa gggcgagaag atctctgata
     6301 tcagcggcgt cgtcgtccaa tcgaacgtgg attatatgtg cgactattta agtcgcaccg
     6361 ccggttccca agcagcggca gcagcggtgg atgaactctg tcgcctcctg aacgcacggc
     6421 ttcccgaccg cacgtaccac gtcacacccg atttccttcg gaacatctgg aatagttact
     6481 cgtatgaatt cgtctgttat ttgagggaat tctgcgaaca gctatccgga gaccctcagt
     6541 tccacatcaa tgtcggaacg gaccgcaagg ttccaccttt gattcagatc ctctgccgcc
     6601 cgttttcgac ccaacagctc tataaaatgt ggccctactt cggacggaaa tatgtccgcg
     6661 atgtgttgga attcgaggtg ggccgggtga cgagacgctc ggcggttctc cgcatgacgt
     6721 ttacagaacg agcgttggtc caatttgggc catatcgaaa acgctgtgtc gacgtcattt
     6781 gtctctcctg caagagcagt atcgcccgcg tacaaaccca gttgcacggc accccggccg
     6841 ctacggtcaa agatctttcg tgcacggccc acggcgatgc gtactgtgaa tgggagttta
     6901 cctggacacc gcaagtccgg agttccatcc cggtggccat gtggacgctg gttgccggcg
     6961 gcacgtttac ctatctccat gtgcgctggc ccgatgttcc cacattcgaa gcgctgggcc
     7021 ttgccgccgt tcccgcctcg ctcctgtggt ggatgattac cagccacatg cggcaggcca
     7081 ccaagccgct tcaacgcctg attaaggacc aagaacaggt cgttgatgcg agacacgagg
     7141 agctgcgcga ggcctacctc gagcagcaaa gtacggccgt gatgttacgt cgaaaagtga
     7201 atgacctcac gacgcttcac cgcgcgggat tattgttcag ctcgacattc gatcgggaag
     7261 aacttctgac cgatgtcctg gatacgatcg tccgcgattt gcactacgat cgcgccatga
     7321 ttacgcaatt cgatcgaacg cgtcaggtct cacacgattt tcgagtgcga ggcatgcctc
     7381 aggaagtggc agatttttta cgcacgcaag aagtgtcggt caccgacccc gagacggtgg
     7441 agggcgccgt gttcttgcgt ggcgaaccgg tgctgacgaa taacgtgcat gagatctggg
     7501 atcggttaca cccgttcgat cagaaattga tcgccatggt caatgtaaaa tccttcatta
     7561 ccgtcccgct caaaacccac gacgccgtga tcggttcact ctccgtcgat cgcacgcaca
     7621 accaagcgct gactcaggac gatcttgata tgttggtcac cctggccagc caggtcgcca
     7681 ttgccctcga taatgcccaa gcctatcgag agatcgaagc cctcaacgcc ggcctggaag
     7741 tccgggttcg tgaacgaacc gccgaattgg aagccgctaa cacccagctg aagcaaatgg
     7801 atcggctcaa atccaaattc ctcgcccacg tctcgcacga attgcggacc ccgctcacga
     7861 gcatcgtcgg gtttgcggat aatatgctcg agggactggt cggttcgctc aacatgaagc
     7921 aggagcaata cctgactcgg atcagagcca atggaaccag gctcgcacga atgatcacgg
     7981 atttactcga cctctcccgc gtggaggccg gcaaactggt gctctccttc gaccacgtgg
     8041 cactgcaaac cgtggtcagt gacgtcatcg agcaacttct cccgttggcc atcgccaaac
     8101 acttgcagat cgaactccgg agtcctgacc cggccctgct cgtctgggcg gatccggatc
     8161 gcgtcagcca gattttgacg aatctcctgg ataatgctat caagtacacc ccggacgggg
     8221 gacacattac cgtagacctc tcagtcggtg atcatgagat ggcccgtgtc gtggtccgtg
     8281 acaccggtca aggcatcccc gccgacgccc tgcctaaact cttcgatccg tttttccgcg
     8341 tgcaccatca agagcggagc cagacgaagg ggctgggcct cggtttggcg attgtgaaag
     8401 atttggtgga tctgcatggc ggcagcatca tggtgcacag tacggtggac gaaggcactg
     8461 aatttacatt cactctgcct ctcgactcac gggcaccgga gcccgctgcg caagtacccg
     8521 ccgctcgccg cagactgctg gtggtggatg atgatgcgga catttgcgac cttttgcgag
     8581 accgtctgga atcagaagga tttcacgtcg acacggcagc cgacggccgg acggccctcc
     8641 gcatcctggc ggagacgccg gtcgatggcg tcttgctgga cattgcctta cccgaagtgg
     8701 atggatttga ggtgttgcgg caactacggc cccagcaacc tgcgttaccg gtggtgatga
     8761 tgaccgccgt ggaggcattg gaccgcgcca tggccgcggt agaagccggc gcacaaggat
     8821 atctcctgaa accattcgac gcggcccgct tgcgacaaat cgtcgatcac tggttcctcg
     8881 ccggcgcaag caagaccgac cccacaagtg gacgataacc ctatgctctt ctctcgcaga
     8941 ttccgcatcg ccctggtact gtccctggtt ctgcttcccg tgagtctttc cctcagtgtg
     9001 acacctgttc aggcgcagac tccccgcctt caagggcaat cggcatccgc cgctgctatg
     9061 ggaaatgcat ttgtggcgca agcagatgat ccctctgcac tgcactacaa tccggccggt
     9121 atgactcagt tgcatggatt ccaaaccctc tttgggacct cgctgatcgg cgggaccact
     9181 caattcaccg gtccaagcgg cgcccaggcg acgggagatc gcaacggaag cctggcctac
     9241 ccgccaccag ggcacgtgta tctggttgcc aatctgaaag acctcggctt cgccgcttta
     9301 ggagatttca gcgccggtat cgggttaaat aaccccttcg gctcgttgac ccgatatccc
     9361 aacgatggac cgtttcgcag cgccctgacc ttcacgacac tgccgctact cgatatcaaa
     9421 cccacaattg cctacaagct caacgaccaa ctatcgttcg gattaggtgc agacatctac
     9481 accttctcag gcctgttcgg tgaagggcat ctcgagcaaa aatcggtctg gcccggagga
     9541 ttaggcattc ccgccggctc actcgttgaa atcaacggga gtgataccac cgcgggcttc
     9601 aacgtcagcc tcctctacac accctatcgc aacagcgagg gcaaacccct cgtcaacatc
     9661 gggatggtct atcgcagtca ggccaccttt catctcaccg gcaacttctt ggcgaatggc
     9721 acttcgatcg ccggtacggc agcgaccttt gtcctgccgc aagtcttttc cggtggcatc
     9781 gccgtctggc cggtacgtac ccctgatcgt gaatggaaat tggaattcga tgtggactat
     9841 gtcggatgga aatcaaaccg aacgctcgat attcatttga cgaacggcac cgtgctaccc
     9901 ttcccgcaaa actggcagag cggttacaca gcaatggttg gaaccgaata tcgatggctg
     9961 cggctggctt cactgccgga ttgggatgtc gccctgcgtg ccggctacat gaatcagcaa
    10021 acgcaagttc ccgatcgaac cttcaatccc ggggttccat cagccaatac ccacattccc
    10081 tccgtcggca ttgggtttgc ctgtcatgag catggatatt tcctgggctt gatccgatgc
    10141 ggtgagttgg gcatcggtcc actcaagcca aaactcttcg ccattgacct gtcataccag
    10201 gccgcgttct atgaagtacg taccgtcacg ggcaatcaga atccgaccgt caacggtcgc
    10261 tacgacacat tgattcacgc cggctcgctc tccttgcgat tcaactactg aaccaatcag
    10321 ccatttctca actcgacacg ccactcgtcg ccccgacttt cgagtacgca agggcacagt
    10381 gattgcttca ctcacatctc gatagcaaca cgagcgaagc aacgtgccca gttccatacg
    10441 accaaagcgg cgtggcgtgg agccggtctc cgatcgtcgg ttgcgttaac cgaccggatt
    10501 tctcgcctcc tggattgtgc ccatccagcg ctgagtagac cgccttcatc tccacgccaa
    10561 ctgccgcgtc ttcgttctca acggagacgc ggctcctttc cccgcactcc cgagcggggg
    10621 ctctcatcga tctgaacgtg tcagaatttc ctgagtctgc acatcactct atccgctatg
    10681 cgctgccttc gcacaaacgc atggcgggcg cattcgtatc ggatccgata cgccacgaat
    10741 ccaatccgat acacctttca actcgacgca ggcccgcata gcggatcggc acgctagcct
    10801 tgatgcgaga tgtcgtgaga atgtctacag atcttcccgc ccgcacatcc gtgctcaaac
    10861 aaccgccgtc catggcacag gccttgcgcg tcacgtagcc aattacaact tctcttcggc
    10921 tgacagtgat gagccaacct caccttcagg agggatcatc atggtcgcgc tcttttttct
    10981 cctactcggg ggcctcgtcc ttctagtcgc agcctgtcgt atctggatga ccgcacccac
    11041 tggaacgttc aaggagacca atgcaccggt agccaacaac tcaggcatgg ccgaaacaca
    11101 gcacagggtt tgaacagaag gctgactcaa cggagtctgg ggctcaacca actgcaacca
    11161 cccagtaatc tgttggagat tccggccgag ccccatgtcg gtgtgggggg ggacaagggc
    11221 ttctatggtg acgagaggat tacgcgccag gcctgtagct ctcccacacc tcgctgtctt
    11281 gcttgcttgt gtgctagcac aagtgttcat gccggggttg tgccaggctc aaccatcctc
    11341 accgatcacc tcctcgggcc tgaataccac cgtcaccaca agcggaaatt cttttaatat
    11401 cacgggcggc actcgtcccg gtggtgggac gaacctcttt catagcttcg gcgaattcgg
    11461 cgtgcccgcg aatcaggtgg ccaattttct caacgattca ggactggcca ccacgaacat
    11521 tctgagccgc gtcaccggcg ggaccccttc caacatcttc ggcaccatcc agacgaccgg
    11581 cttcggcaac gccaatctgt ttatgatgaa tcccgcgggg atggtcttcg gacccaatgc
    11641 ctccttgaat gtcgggggct cggtgcattt tacgacagcg gattatctcc gcttggggga
    11701 cggggcccgc ttcaacgcca tgccggggcc gcaggatgca gccgtctcca gcgcaccggt
    11761 cgcggcattc ggctttttag gatcaaaccc ctccgccatt gtggttcaag ggagtcaact
    11821 cactctcgcc gaaggcaaca ccctctcact gataggcggt aacgtccaaa tcgccaacgg
    11881 gacattatca gatggcgcga tccagcccgc gcgcctctcg gcaccgggag ggcaaatcaa
    11941 cctggtcgcg gtcggatccc ctggggaagc gttgattagc aatggagcgg ggacgctcgc
    12001 cgagccgatg ttgaccggct tcaccggact cggaacgatt tcgctttcgc acggcacgac
    12061 aataggcaca agcggcgatg ctgcagggcg catcgtcatt cgtggcggac acttcagcat
    12121 ggaggatgca ggccttgagg ccatctcgac atccgtctct ccaaactcgc tcccctcttc
    12181 ctccccgaga ggggatatct cgattcaggc ggaagaggtt tcgttgtcga acggcgctca
    12241 cctacttacg tccacgatca atggcacggc cggtaatatc gcgctggagg tgggtactct
    12301 gcgaagcaat gtcggaccgg gcggcctccc gctctcgggt gccgcgccag tcactattac
    12361 cagcagcagc acagggctag gcggacccgg aagcatttcg gtcacgggca acggacgggc
    12421 ggcttcggat gttgtagtcc tcagtaacac acaggccgtg actactgtgc ctcactccgc
    12481 tcaaccgaca attgtaccag cccacattga tatgacggca gagcatctcg aactcaggaa
    12541 cgggacggtc ctcaagacgg ataccacggg aggagctgat gccggatcga tcacattgaa
    12601 agttggaacg cttgctacgg aagccggtcc tgacggaaga gtgcttctct caagcagcag
    12661 taactgcggg accggatgtg tggggggaca ggccggtgac attaccatcc agggcattcc
    12721 aggaatcact ccggcggaaa cccacttgta tgtccccatc gtcagacctg actcccctcc
    12781 caccgtggcc attagttatc agttggcgag aaatatggac cttcgaggca cagacattcg
    12841 ctccgaagcg atcggtaatg cgccgggcgg acaagtcatc atgagggcgc agggatccat
    12901 ctcgctcgcc gataccacca tctcggtcgc gacacaggac tttcaaatcg acggacctaa
    12961 gccaagcggc gaacttgtcc gcaaccaagg gttcagccgg attgatatcc tcgcgcatga
    13021 tgtcacgtta aaagacagca ccgttcgggc agacgcaaat gtctcggata tcggctcctg
    13081 cccgctctgt ctcggggggc ccagcgcagg agagatttgg tttcgagtcg gcaattcatt
    13141 cactgccgat aattccgcca ttattaatag tggcaaaggc agagcccagg ccgggctcac
    13201 gaaaatcatc agagaccact tcttttcatt tggcgccatt tgggagccgg attaccccga
    13261 ttatcctacg aactcgatta ccctcaatca ttcccaaatc accgtcgaat ctccgcattc
    13321 agggattccc ggatatcttc gactccgcgc cgacaacgtg acgttgaacc attccatcct
    13381 caactctcaa gtcaacgacg tgtcgaactc gcgagggctg gacgggcaat tgatcgatgt
    13441 ccccggcgcc ggcgaacgcg gacgagtgat tgcagacggc cgggacgtac agggcagcgt
    13501 cttggtttcg gcaaagacgc tcgacatcac cggcggcgga attatcgcgc caaccacagg
    13561 cagccgcatc gcgagtcgca tcgagttgct cgcgaacgag ttgacgaccc acaggggaac
    13621 tgggccggga ggaacgtttg atgccccacg catcttagat gccgccgacc ccacgcgcgt
    13681 ggttctgtcg agcagcagca cgggaagcgg aggcgcaggc aggatcagca tcgcaggcga
    13741 gggcgtggct atgcccgagg gcaccccata ccggcccgcg tcgtccattc atctcgatgg
    13801 cacggccctc ttcaccgata cgcacagcga tgccctggga ggcaaaattg agatgatctc
    13861 gcgcggccca atcgtcctgc atgacaccac cgtctccgcc aatgtcacag atgtgcggcc
    13921 ggcttctgcc ggcttgcaag agcaaggtgg gaacatcagt ctctcggcag ggagtctctt
    13981 ggtacaaggt ggcgggcttt cggcactctc tcgcggcaca cagaccggcg gcaacatcgc
    14041 gatcacggtg caaggcccga tcacgctgga atccggtgcg gcaatttcgg ccagtaacac
    14101 ggggtcggca aatgccggca gcgtcaccat ccacgccggg tcagagtttg tgagccggga
    14161 tgcttccgtg acgacggaat ccattcacgc aagcggtggc aacatcttca ttcaagccac
    14221 tgattccata cgactcgtga atagccgcat cagtacctct gtgctaggtg gtccgaattc
    14281 atccggaggc aatatcattc ttgatccggc cgtggtcaca ctgcagaaca gtcaggtact
    14341 cgcgcaagcg gtgcaggggg caggcggcaa tattcgcatc atcgcaggga cattcctggc
    14401 cgatcagacc agcgccgtca gtgcgtcgtc cgaattcggg ttgaacggca ccgtctccat
    14461 ccaatcgccg gtctccagct tgagcgggac gctcgcgacg ctcccgcaaa gcaccctgca
    14521 aacacagcaa cttgtcacac aacgctgtgc cgcccaggga agtggtcatc tcagcagtct
    14581 cgtcgtcgcg ggacgcgatg tgctgcccgt cgaaccaggc ggatggttga tgagcccgtt
    14641 aagcgtcttc gctgtggatg cccatgatga cggaggtcgg cctgtcgctg agacgattcc
    14701 tgatgcagcc gaagggggca acaccctggt ggaactgacg cagggacatc cgactcctga
    14761 gccgcgctcg aacttcacta ccccgggagg aggctgtcgc tcataacggg ttgagccggc
    14821 gcgccggcgg acaggtacaa cacttggctt tgcgcatcgt tacggtccca tgtgtatgtc
    14881 gcgttgtggt actgagcctt tgccttatga cattccaagc cctggctcaa gtggcgcctc
    14941 ccctcccgcc gatttttgac ccgaccggga aatcggggaa accgccggcc ctgctcaaag
    15001 aagacttcaa tactcctccc tcggagccac ccagctcgat actccccaaa gtaccgacta
    15061 cgcctcccgc gctgcccgac aataagttgg gggcatttcg agtgttcgtg catgatatcc
    15121 atgtgaccgg cagcaccgta ttttctgaaa ctgaactttc agaagtgacc gcgccgtatc
    15181 gcaatcgata cctcacaaca gaagatttgg agaaggtgcg actcgccctg acacttttat
    15241 atgtgaacaa cggctatctc acatccggcg cagtggttcc ggaccaggac gtgacgttcg
    15301 gcacgattgt cattcagatg gtcgagggga aacttgcgca catcgacgtc gaggggaatg
    15361 cgtggtttcg aaatgcgtat ctccgcgacc gtgtggcgcg cggggtgtcg acgccgctca
    15421 acatccaccc gcttcaggag cggatacaac tgctgcaaca agaccctcgc atcgaacgga
    15481 tcaatgcaga actgaaacct ggagacgttc gcggcgagag cgtcttgaac gtgcgcgtga
    15541 aagatgccaa tccctttcgg gcctggctgg agttcaataa ccatcaaacc ccgacggtgg
    15601 gcgccgaacg cggtctggcg accgtggcgc atcagaacgt gaccggtcac ggcgaccaat
    15661 tcatgtttac ctacggccgc tcccacggcg tcaatcccat catcgatacg tcctatagcc
    15721 taccgctcaa caggtatgag acgacgctga ctgcctacta tcgacgcaat gcctttctcg
    15781 tcgtcgaaaa tccttttcgc gccttggatt tgaacgtgga ctcccaggtg atcgggctcg
    15841 ctctgcgaca gccggtctat cgaaccgtca ccgctgagtt cgcactgacg ttgacggggg
    15901 agcatctatt tttgaagacc acatccgctt tcgatgctcc cggccttccc tcgctgttca
    15961 ttcccggttc gtccacgaca ggagtggcca ccgtcagcgc actgcgattc agccaggagt
    16021 acacacatcg ctcttcatcc ttggtatttg ctgccctatc ccgcgtcagt gtcgggttga
    16081 atgtattggg cgccacgatc aatagcggac cacttcctga tgggcagttc gtgtcctggt
    16141 tgggccaggc tcaggtcgta aagcgattgg atgactggtg gggcgtgcaa ttgttggggc
    16201 gtgtggcagc gcagttggca agtgaccgac tcttccccct ggaacaaatg ccggtcgggg
    16261 gccgtttcag cgtgcgcgga tatcgcgaga acacgcttgt gcgggacgat gccgtgctgg
    16321 cttcattcga gtcgcgagtg ccgttgctcc ggttcccatc cggagaagac cggctgcaat
    16381 tcgccccgtt cgtcgatgtc ggccacgcct ggaacgccaa aggccacacc ccagatccgc
    16441 atacgttggc gagtgtcgga gctggactcc gttggatgat cctccctcag gagcgggccc
    16501 gcttcgaggt gtattggggg cagcaactca atcacgtccg cggcggggaa ggcaatttgc
    16561 aagatcatgg cattcatctc caacttgttg tgcaggtgct gtaaatgagg catcaccggc
    16621 tcacagtatt cccgccgccc tcgcatctgc ttcgatacgg actcaagggg ctcagtctgt
    16681 gcctgattat cttcttctgc tgtaccgttt ctctggctgc ggacagtcag ccttcggctg
    16741 gtgacttgat gcagcgcggt cgagcggcgg cccgagaagg atcctatgaa gaagcgctcc
    16801 tcgcatggaa ggcagccgcg gagttgtacg accaggccgg tgagacgaag aaccatatcc
    16861 aagcgctgac acagtccgcg tttgccgctc gtaccttggg gcatatcaac caggcgtttc
    16921 tccaagagga actcgcgctg cacctcgcac gccagctgcg cgattctcaa tggctggtca
    16981 tcacgctctc cgaattaggc aagacctatg tcacgaacca tcagtatgag gtggcagcgg
    17041 actatattgg tcaagcactt gaaatcgcac aggcacagtc attccgggcg ctgagtcccg
    17101 cactccagaa tgatctcggg atcgttttgg ccttgcaggg acgcccggaa gaagccctgg
    17161 cggcattcca ccaatgtgca gtgagtgcga aacaagaggg gctcatcccg ctcttcattc
    17221 gtgcccgtgt caacgcggcc cggatggcga ttcaggttgc gcagttcgat acagccacat
    17281 cggccctggc cgaagctgca gatcaacttg ccggctccgc ctcctcctac gaaaaggccg
    17341 aaggactcgt gaatctggct ttgggatatc gcgatctggc cttggctcat tctggctctc
    17401 acgcgtccgc cctcaagcga tctgcgctct tcttgcagga agccgcaggg atgacacaac
    17461 acccgggcga cgcgcgcctc gcatcatacg tttacggcca tctcgggcac ctctatgaag
    17521 ttgaacgccg gtttgatgaa gcccttcagt tgactcgaca agcggtcttc accgcgcagt
    17581 cggctcgcgc accggagtcg ttgtatcggt ggcaatggca aagcgggcgg ctgctgaatc
    17641 gtctcggcca actcgacgac gccctgtcgg cctatcaaga agccgccatc actctgcatc
    17701 ccattcgaag cgaggtaaca ttcgccgtcc agactagccc ggattcgcat cagccatcca
    17761 tccgcgcgtt atattttgag ttagccgact tgcttctgcg gcgagccgcg ttgatgcatg
    17821 aaaaccgaga ggccgaggcg tacttgcgtg cggcccggga tgtgattgaa gcctttaagg
    17881 ctgcagaact tcgagactac tttcgagacg actgcgtcga tcagatgcag gcccgcatca
    17941 caaaactgga cgcgctctca ccttccactg ccattattta tcctatcgtc ttcagcgatc
    18001 gcaccgagct cctcgtcagt cttcctgacg ggctgacacg tcattccgtt ccggtgacgg
    18061 ccgccacctt gacggatgaa gtgcgtcgct tccggcatac gctcgaaaag cggacgactc
    18121 gtgaatacct ccctcacgcg cagcagctgt acgattggct gatccgaccc cttgatcctg
    18181 atcttcgccg gctcaaagta aacacattgg tctttgtccc ggacggatcg ctccgcacca
    18241 ttcccatgtc ggccctgcat gacggaaagg agttcctcat cgagcactat gccgtggcca
    18301 tgtcccccgg ccttgatttg acggaccctc aaccgattga tcgtgccaag gtgcatctgt
    18361 tgtccagcgg gctgaccaaa gccgtgcagg ggtttcctcc attgccccat gtcgccgacg
    18421 agctcgattc cattcgtgcg ctgtttaagg gagagcaact catcgaccag gagtttctga
    18481 cgccccggtt ggaagaggaa ttgaaggggg ggcgttacag catccttcac atcgccactc
    18541 atggacagtt tgccccggac gtcaatcagt cgttcctcct gaccttcgat gcccgtctga
    18601 gcatgaccca gctggagcga ctcgtcggcc tgtttcgttt tcgacgagag ccgcttgaat
    18661 tgctcacctt gagcgcctgt cagaccggca gtggtgacga tcgggcagcg cttggactgg
    18721 ccgggatcgc cgtcaaggca ggtgccagaa gtgccctcgc cacactctgg tccatcaatg
    18781 acgacgcctc gtccgaactc gtctcagaat tttaccgtca actgcatgac gcatcggtct
    18841 ccaaggcgcg agctttgcag catgcgcagt tgaagttgct gtcagatcgg gtgtatgaac
    18901 atcccgccta ttgggcggca tttttattat tgaacaattg gttgtgacgg actctggaac
    18961 agggtgatcg cttggcgacg tggctctacc gctccatggc tcgaacattc gtagccacac
    19021 tccttttcac cgtgtcgttg gtgagtacca atgcggccgc gccggaaggt gtgccggacg
    19081 tcccccagta caatgcaccc cagaagatga cgccccgggc acgtctgggc ggaagcttac
    19141 gcggcagtga gggtgaagat cctgccgtcg cggcgcttgt cccggaccac gtcggcatca
    19201 ccgtcaaaca gcatccggca ttaaactggt ttttgtccaa gcatacgtca ttgccgatca
    19261 cttttaccct gatcgatgag cgctccattc atgccttggt tgaacgcccc ctcgcgccgc
    19321 ctcatcaagc cggtgttcac gccgtcaagc tcgaagacct gggggtgacg cttcaaccga
    19381 acattcagta ccgctggtat atatccgtga tcaaagatgc cgactcgccc tcacaggata
    19441 tcgtcacggg tggaatgatc gaacgctgcg aattcagcga gtgctcgatc ctaggcgcca
    19501 tgacaacatg tacgcgcgaa gccgtcatga ccagcgccgc caaaggattc tggtacgacg
    19561 cgatgggatg cctctgcgat ctcattgact ccaatccggc agataccgcg ctccggaagc
    19621 agcgtgccgc cttgctcagg caagtgggcc tgcatgatgt ggcggactgg gatctcgcac
    19681 aatcccatgc gcgacaaggg tgacgcatgc cataccggcg agtcagtttc ccactggtct
    19741 tgatgatcgc gtggctgacc ggggggtatg cgtcgcagtt tccgttcggc gcccctgata
    19801 gtatcgcggc ggatcctctc gtccctcttg aattgcgcct gcccgaatac aaaccgaaaa
    19861 aggcagatgc ccctcgagcc cgcatcgggg gacacagccg tggaacagaa gccggagccc
    19921 ctgtcctcat tgctctggtt ccggatcaca ttgcctttac agtcaagaat gatcccaccc
    19981 tctgttggta ccagtctttg catacctctc ggccaatggt tctgacggtg gtcgattccc
    20041 gcggtatccg ccccgtcctg gagcattcac tgcccacgcc agttcgtcac ggaatacagt
    20101 gcctgaggcc gcgcgagtat ggggtcgaat tcagagtaga agagtcgtat cgatggtatg
    20161 tgacggtggt gatggacccc aacagaccct cgctcgacgt cgtcgcaggc ggaatgatcg
    20221 aacgactctc tctcgatgaa gcctgcgctc tcggcatgcc ctgcccctcc tcctcttgcg
    20281 ataccgacgg gatttatcgc tatgcggagt caggtctctg gtacgacgcc attgcctgtc
    20341 tggtggaact gctcgagcag gagtctgaca aggataggct gcggctgatg ctggaccatt
    20401 tgctgcacca atccggtgtc cacctgccgg gagagtcgtc accgtaataa caacgttgag
    20461 actcgcactc accggctctc tccattcttc actgccactg cttctagccg tctaccgatc
    20521 agcgcgcctc cccctaaaaa catgtagccc gctgatcgtt gtccgttatt tgattttctc
    20581 ggttgaagac caccgtgatt gggtatctca tcagatacgc taggatcgga tccgatacag
    20641 tgagacgggc aaccgctcag ggatacgact gttacctcac tcgactttcc aaacattttt
    20701 tccatcgagc cggagctgtg cgtaccgatc gttgtggcac ggctgttgct gtacctttgg
    20761 acatgtagac gattctttgg gcggttgtag aaagacacag ccccccttcg aaatagaaag
    20821 gagcctgaca tggaactcgt catgggcggt ctcctggtgg gcgtcagtgg catgttggcc
    20881 gtcttgatcg tcgcggtctg gaaagacagc gcctctcatc gtacgggggc gcatccgtca
    20941 caccggaggg tttagccgct tgatgttcgt ttgaacgaag cggcgcggca gaaaaacaca
    21001 gccagaacgg atcgggaggc gtacacctcc atacgcctct cgtgcaaagg agttgcacca
    21061 tgaaccggtt cgtgtatttg agacctcaaa agcgccagcg catgactgtc agcctcccgg
    21121 cggatctttt agaacggatg cgcgatgctg cgtactggac atccgggacc accatggccg
    21181 gcctcatttc gtcggccatt gaagatctgc tccacaatct ggaatcgcaa aatggccgac
    21241 cgttctcccc gcgcctccag gacctaaagc caggccgccc caggtccaat aaaccggatc
    21301 aatcgtctgt atctgaaaac atacagtctg tatcccgtta gatacgcaca ggctcgccac
    21361 gattccccgc gccaccttcc ttcgtgccac gttcatccga atcaatcgga tcttttcgga
    21421 atctcaaccc gccgtttctt tctctatccg cacgaaacaa gcatgccttt tgcgagagaa
    21481 tttgttgagt caaagtgggt tcactccttt tctccgtcag acaacgtccg actccgccgc
    21541 taaagggctc aaggtatgcc atgcgcactc ggaccgcacg agccgaaacc atcgtccatt
    21601 gtccatttac tgccacgcaa cctgagcccg cattggcggt acacccaatc acgcttcgct
    21661 gggatcacta tacgcgcgca ttggcgagag gtctcatgag cgtatgctgg gccttcgcca
    21721 tcgaggcaca ttcgccgatc cccacctatg ctgcaccaca agcagacgcc ccggcaaacg
    21781 aagagctgga actactcaaa gaagaagaaa cggttagcat cgccacgcgt cacgagcaac
    21841 cgatttcaca agcgccttca aacgtatacg tcattacgga tgaagacatt cggcattccg
    21901 gcgccaccga tattcccacc attctccggc gcgtgccggg gctggatgtc atgcaagtca
    21961 ccggggctga cttcaacgtc agcgcacgag gcgacaacca gctcttcgcg aataagattc
    22021 tcgtcatggt ggacgggcga tcgatctaca tcgacggcca ggggctcgtg ttctggaaat
    22081 cactgccggt gacgctgccg gagatcaaac gcattgaggt tctcaagggg ccggcatccg
    22141 tggtctatgg attcaatgcc ttcgacgggg tcatcaacat cattaccaag tccgcgcagg
    22201 agatgaaggg tacgacactg cagttcggtg gtggggaact cggcaccatt tccagcgctg
    22261 cggtacaggc cgggtcggca ggcaaactcg attatcgcct atccatcggg cacgaccaga
    22321 atcagcaatg gcgaaacaat gatgcgctgg cgttccgatc gaataaattc aacgcacaga
    22381 cacaatacaa cttctcgggc gacgccatct tgcgcgtcgc tggcgggttc attgatacaa
    22441 acaggtttga cggtcccgtc tcaaacgtgc aagtccctcg ctcccaattc aatcaaggct
    22501 attccgacgt ccactaccaa tatcggaact tctctcttcg cggctggtgg cagggcaatg
    22561 acatccccgt ggaatcttcc acccatccgc tcctcgcccc atttgtccgg cagaccaatg
    22621 cgacgggtgg ctcgaatgtc tcgttcacca cgaataccta taatatcgag gcgcaacaca
    22681 gtctcgactt cggcccggcc aaccggctga gttacggcgt caactaccgc tataactccg
    22741 tcgacggcgc agccgtctca gaattgggcc gtgaagatcg actgggattc catctgcagg
    22801 atgagtggaa aatcctgtca tcgcttactc ttgtggcggg agtccgatac gacctgcata
    22861 cccgaatcaa cggcacctgg agcccgcgcg ttgcccttat ctacaatctg actccggagc
    22921 atacgttccg tgtcagcgga tcagtggcat accggccacc tacgctgttc gagtccaatc
    22981 tggacctgcg aatcttaccg accgtccctc ccctgttcgc ccccattccg ctcttcggtt
    23041 cacagaggct caatccggaa cagattattt cctatgaagc cggctaccag gggtggtggc
    23101 taaaacaccg gcttcggacc aggctcgatc tgttttttaa ccatctctca gacctgatca
    23161 attttcaaac catcggtgcc acacgcacgt tggtgaacgg cggggaagcg gacatctacg
    23221 gaaccgaggc tggatttgaa tttctggcga cgagatggtt gagcggcttt gccaacgtgt
    23281 cttaccaaaa gatcgggcag acctttaccg gcgtctcacg tcgaggcgga ccagactgga
    23341 aagccaatgt ggggttgcgt ggcgactggg acaatgggct caatggtgaa attgcggtgc
    23401 attacgtcgc aggcgccacc taccccctga ttgaattgtt tacggccctc gctccggtcc
    23461 tgcccgcgcc gatcagcccc aacatcgata gctatacctt gttgaacctc cgcggcgggg
    23521 accgattctg gcatgagaaa gccgaggtcg ccattgccgt ctataacgcc ctgaacgacc
    23581 gccacaagga acatccgttg ggagacacgc tgggaagccg agtcatgggt tggctcacct
    23641 tacggtattg agttatgcat gctgaatcca agggagtgtg gcagacgatg gtcgcggcat
    23701 tcatgacagt gagcatcagt cagttcgctg ccgccgaacc tccggccatc cgggtcgagg
    23761 attccctgac cgttcccgcc aataacgatc tggagttgtt gaaagaagag gaaaccgtga
    23821 gcattgcatc acggtacgaa caacccattt cgcaggcacc gtcaaacgtc tacgtcgtca
    23881 cggatgagga catccggcag tccggcgcca ccgatctccc aaccgtgctc cgtcgcatcc
    23941 cgggtttgga agtcatgcaa atgaccggcg ccgatttcaa cgtcagcgcg cgaggtgaca
    24001 atcagccctt cgcaaacaag atgctggtca tggtcgacgg acgttcgatc tacctcgatg
    24061 tgcaaggcaa cgtcttctgg aaatcccttc ccgttacact cccggagatc aagcgcatcg
    24121 aagtgctgaa aggccccgcg tcggccatgt atggatttaa tgccttcgat ggggtcatca
    24181 acatcatcac caagtcacca gaagagatga aaggcaccac ccttcaattc ggcggagggg
    24241 agcaaggcac catcagcagc gcggccatcc aggcgggtac agtcggcaag ttcggctatc
    24301 gactctctat cggtcgcgat caaactcagc aatggagaga cggcgacgcg cttgcgtttc
    24361 gcgcgcacaa attcaatgtc cagacggact atgcgttgtc ctcgacgtcg aagctgcaac
    24421 tctctggagg gctaatcgaa acgaatcggt acgacgggca agtcggtgaa gtcaccagca
    24481 attccattcg cccttccttt gggtacgcca atgcggtcta cgagcacggc gcctttctcg
    24541 ttcgtgcatg gtggtccgga tatacagatc atgccaccat caaccctctt ccccaattgg
    24601 ccggccttct cagcgtgacc gatcgtaacg gcgtatcgac caatccgttt tcaggcaata
    24661 cgtacaatgt ggatgtgcaa catgcattgg atatgggtgc ggccaatcgc ctcctctatg
    24721 gtgcgaacta ccggcacaat tcgctttcga gtccggccat cgatcaattc agcagggaaa
    24781 accggctcgg gttgttcttg caggacgaat ggcgggcatc ctccgcgatt accgtcgtcg
    24841 ccggtatccg gtatgatctc cacagccaga tcaacggcac ctggagtcct cgcctcaccc
    24901 tgttgtatca accggtagag gggcacactt ttcatctctc gggatcggcg gcctatcgtc
    24961 ctcccacgct cttcgaatcc catcaggacc aacgagtcac gacgaatatc ccgaccggtt
    25021 tacccttccc attaccttcg tcagtctctt caaccgtacc ggtgacgggc tcgaccggtc
    25081 tcgctcctga acggatcatt tcgtatgagg ccgggtatca gggttggtac tggaaacatc
    25141 gcatacgcgt acgggcggat ctattttata accacgtctc ggacctcatc ggcagccgaa
    25201 tcaccgcgag cgggtcttct gagtttgtga acgatcccgg atccgccgac atctatgggg
    25261 gcgaagccgg catcgaggtt ctggccaatc gctggttaag cggattcgcc aattatgcat
    25321 atgaggagat cggccagtcc ttcaccggaa cagtccgccg gggcgcgcca cgatcgaagg
    25381 tgagcgcagg ggtccgcacc gaatgggata acggcctgag cggagagctc agttattact
    25441 acgtgggcgc ggccacttat ccgcttgcac aaagcttcac ccaactcggc actattccgg
    25501 gagctggcat cacggtgcct ggaaaccagg tgggcagcta taacctcctc aatctgcgtg
    25561 ccggatatcg attctggcaa cagaaggcgg cggccgggta tatgcgtgat gcggaggtgg
    25621 cgatctcggt cttcaacgcg ctcaatgacg aacacaagga acaccccctg ggcgacttga
    25681 tcggccggcg ggtgatgggt tggctgacgc ttcggtttta acctgtgccc gacgacactc
    25741 gggaggatgc aatggcaccg gcaacactga ctcattccat cctggttgtg gacgacgatc
    25801 ctgatattgc gcttgggctt caggatttcc tcgaccacga cgggtatcaa gtgaacgtcg
    25861 ccatgacctg cgcggaagcg ctcgcccaag cccacgccca tcactacaat gcggtattac
    25921 tcgatctggg cctgccggac ggagatggct cttccgttct gctcacgctc caccaacaac
    25981 agccgcaact gccggtgatc atccttacag cttataccag caccgaccgc acagtcggct
    26041 cgctgacaca aggtgccttc gcctacctga caaagcccta taatcgagat gaactacgcg
    26101 cggtcctgca acgggcagtg ggcgtccagg ccctggccgt caaggctgaa catgccgagc
    26161 atgccctgca tgagagcgaa gcacggtttc gttccctcgt cgaagcggcg accgactcca
    26221 tcgtcctcgc cgatcacaat ggtcatattc tttcctggaa tcgcgcggct tcgcacctgt
    26281 tcggatacac ggatgaggaa gtccgcggac agtccctcag catcctgatg ccaccccggc
    26341 atcgcgccgc tcacgaacgg gggctggcgc gggtccgaga cacgggggaa tcccgcctca
    26401 tcggccgcct cgtcgaactc gaaggattgc ggaaggacgg gagcgagttt cctctggaac
    26461 tctcactggc catgtggaaa agcgacgaag ccgtgtttta cagcggcatc atccgcgaca
    26521 ttacacaacg acgccgcgcc gaggaaattc ttgaccgcct gcggcatctc cacgaggtca
    26581 tcctcactca ggccggcgaa ggcatctatg gcctggatcg cgacggaatc acgacgtttg
    26641 tgaatccgac agccgccgaa ctactcggct acgaaccgtt cgaactgatc gaccggccga
    26701 tgcatggcct gcttcatcat tctcgatccg acggcagccc ctatccgtca ggtgagtgcc
    26761 ccatctatgc cgcattgcac gacggactgg tgcatcgcgt ctcggccgac gttttctggc
    26821 gaaaggacgg tcaccccgtg cctgtggaat acgtcagtac gccgatcatc gaaaagggac
    26881 gcacagcagg cgcggtcgtg gtgtttcgcg atatctccga acgcaaagag gctgagcgcg
    26941 cagtcgagga aagccaggag cggtttcgtc aactggcgga acatatccga gaggtgttct
    27001 ggatcacgaa tccagaaaaa tcgcatgttg tgtatatcag ccccgggtat gaggaaatct
    27061 ggggtcgctc ctgcgatagt ctttacgccc atcccacgtc ctggctggat gccattcacc
    27121 ctgaagatcg gcctcgaatc gaagaagccg ccataagccg acaagcgttg gacgtgtaca
    27181 acgaaaccta ccgcatcctg cgtccggacg gatccatgcg atggatctgg gatcgcgcct
    27241 atccgatccg tgacgcctcg aatcaggtct atcgcatcgt ggggttcgct gaggatatta
    27301 ccgagcagaa acggatcgag gcggaattga tcgctagcga acgacgctat cgggtcctct
    27361 tcgacgacaa cccgtctatg tatttcatgg tcgatgccgt tggcaccgtg ctctccgtca
    27421 atcgcttcgg agccgaacgg ttgggctacg aggtgcagga tctcgtcggg acctccgtcc
    27481 tcaacgtgtt ccatccggag gatcaacagg ccgttttaca caatctgtcc gactgcctct
    27541 ccgaagtggg acaaccgaag cgctgggaac ttcgcaaagt ccgaaaagat ggccacgtga
    27601 tctgggtgag agaaaccgcc caggccgtgc ggggacacga acaagccccc gtggtgctga
    27661 ttgtctgcga ggacatcacc gccatgaaag aggctgaaat cgcgcttcac gacagtgagg
    27721 agttaaagaa ccagatcctg cacagcagcg cggactgcat caaggttctc gacctggaag
    27781 ggcggctgca atacatgaat gatgccggac agacgttgct ggagatcaag gatcttggca
    27841 cctacaccaa caaaccctgg tcggaatttt gggagggaga cgatcgggag gctgccttag
    27901 ccgcgctgga ggcagccaag gccggggaca ttggaaagtt cgtcgggttc tgccctacca
    27961 cgactggaca ggcgaaatgg tgggatgtgc aggtcactcc tatgcttgac acacaaggct
    28021 attcccggcg tctcctggcg atttctcgcg atattactgc gtataaacag gtccaagaat
    28081 cgctccacgc cagcgaggaa cggttggaac acgtcattcg cggctcccac gacgggttct
    28141 gggacggcca tgtcttgccc gatcatcctt ggtacgcgcc tattaccccc gtgtggtggt
    28201 ctccccgcgt gcgcgaaatg ttgggataca atgaagaaga gtttccggac attctcgaca
    28261 gctggatgtc ccgattgcac cctgaagatc gtgaagcagt ctttgagcgg ctgaatgcct
    28321 gcatggaaca aggcgtctcc cagtacgacg tcgagtaccg gctcttgaac aagcaacatg
    28381 agtatcaatg gtttcatgca cgcgcggagg tggtccagcg cgatgcacaa ggacaggccg
    28441 tcaggatggc cggcgcattg caagatatca ccgatcgcaa acgaaccgag gcccgactgc
    28501 ggctcagcga agagcgcttg cgcatggccc tgacctcatc ggatctcggc atctgggact
    28561 gggatgtgac ctccgaccgt ctgtattggt cagaaggcgt cgaactgcta ttcggcttgg
    28621 cgccagggtc ctttcccggc acctataccg actatattgg attgatctat ttgttagatc
    28681 gcggatctac tcttactcgc ctcgaactca gtcttcgtga tcagacttcc gtccatattc
    28741 ggcatcgcgt cgtatggccc gatagctcga tacattggct ggcatggagc ggacggatct
    28801 atcggaatgc cgacggagtc gcaacgcgtg tactcggcat cgtgcacgat acgacaggac
    28861 gcatggacga atgacctggc gacattcggc gacctcatac cgggcaggct gattcgacga
    28921 ggacgcagat tgtgacgtgt gcattcctac aacggacgtg tgacagcagg gcatcaggac
    28981 gcctttggaa caggctatgc tggagcatgg ttctcctctt gtggaccttg ctgatcgcca
    29041 gtcctgccct ggcaactgac gtcatcattc tcaaatcatc tgatatcgcc gcctacaatc
    29101 aagccatcaa tggcttcaaa gccgctatcc cggccggaat caatctctcc gaatacgact
    29161 tacagggaga ccttgagaag ggtcggaaat tggctcgaaa aatccgcgca tccgatccgt
    29221 cattggtctt cgccgtcgga ttgaaggcgg ccaaggcggc ccaacttgaa atcgtcgaca
    29281 tccccgtcgt gtactctatg gtattggatc cggccaaata cggtctcaac gctcagaata
    29341 tgaccggggt cctgctggaa gttcccgcag aacggcagct agtcatgatc cgcaacctac
    29401 tgcctcaact caaacacatc ggcacgctgt atgacccagc caagacctcg acgctgattg
    29461 acgaagccag acgtctactg aaacagagcg gaaccgaact ggctccgatg caaatcagca
    29521 gtgaacggga tgtgcccgga agcttgcgtg ccttactgcc ttcggtcgac gcgctatggc
    29581 tcgttcccga ttccacggtg ctgacggacg agtctctgcg ctttatctta aatacggcac
    29641 tggaagctcg ggtaccggtg attggcttct cacgcgagtt cgcgcggagc ggggcgttat
    29701 tgtgcctgtc cgtcaactac gccgacatcg gacggcaggc cggacaactc acacgcaagc
    29761 tgctcgaggg ccagctctcg ttaccgctaa aaccctggca ccccgaccgc atcgaaacca
    29821 gcttgaatct caaaacggcc aaattcctcg gtatcgacat tcctcgtgaa ctcgagcaaa
    29881 aggccgacga gctgtattga tgaaaggacc gtctgtgaaa gatcccgccc atcttgccgc
    29941 cccgccgttc ctcccgaacg ggctgaaacg attcgtcagc ctgcgaacca agttcgtcgt
    30001 gttttttagt ctgatcatca tcttcacgtg ctcaggtatg agttggtatt tcatccatag
    30061 caaacgcgtc gcgatgaccg aacgggtcca ggacctcggc agcatcctgg tgaagaacct
    30121 cgctcacaat gttcggtacg gcatcattct cgaagatcgg gtcattctgg aacaattcat
    30181 cgacggggtg ctcggagtgg atgaagtcgt ctacgcgcag atcaccggcg ccgatggcca
    30241 ggtccttgcc gcgaagagca aaggcaaatt gaagacaccg ctcgggacgg tgcgcgccgt
    30301 cgatcagccg ttttacccgt ccgctgcgat cgcggcatcc ctgatcagac atccgctccc
    30361 ggaaccgact gtgacacggc tgcgactcat tggcgaaagt agcctcccct ttgaagagca
    30421 agacgccacg cttcgcgtat caacggccgg catgcgagaa gaaacgtttt acgattttgc
    30481 gcttcccgtg ctcaaacgcg ctgaaccgag actggaagca ctggcgcttc aagaggaaga
    30541 gacgcacaag acccgcaccg cggcgtcgac ggcgcctgcc tttggcgtcg tccaaatcgg
    30601 cctcactgag gtacccatgc agcgcgaact ggcggaggtg ctacggacct tcctcctcct
    30661 cactttgggc attattacgg ccggcattct ctccaccaat cttcttgccc gccgcatcat
    30721 tacgcccttg cgcaatctcg cggcgggagc ccgcaaagtc ggcgaaggcg atctctccac
    30781 ctttgtcgtg ccgaccacac aagacgaagt tggccagctc actgcgctct tcaatgtgat
    30841 gaccaagtcg cttcaggaac gtaatcaggc gatctctgca aacctcgaaa ccatccgccg
    30901 ccacgtcacc cagttgacga ccctgaacca atccagcgcg gcgattacct ccaccctcga
    30961 tctggatcgg ttgctctcta cggtgctgca gcttctgagc gaaaatctcg gcttcgccag
    31021 aatggtcctg ttcttgtggg atagcgagcg cggtgtcgcc acggtaggac aaatgctggg
    31081 catgccaccc gaggctgaag aagccgcacg tcgtctggtc atgcccgtgc atgaggacgg
    31141 cgggttcgcc gcggaaatcc tcctccacgg gaaacccgtc ctggtgccga atatcgagac
    31201 cgtcgccgac cgcatctctc ccgccgtctt gccttggctt cgccaggtcg gattgtcgtc
    31261 atttgtcgcc gcccctctcc gcagtcagca acgcattctc ggatatctcg gggcggatcg
    31321 cggcgcacag ccttgcaccc aggaagatct cgatctgctg gtcaccatcg ccagccatgt
    31381 ggcggtcgcg gtggacaacg cgcgggcgta taccgaactg gccaccttga cggaacatct
    31441 cgaacgacgc gtggaggaac ggacgcatga attgcaatcc gtcaacgagc gccttcagga
    31501 ccacgatcag cgacgctcga agtttgtttc cgttgcatct catgaattgc gtacaccgat
    31561 gacgtccatc aaagggttcg tcgaaaacat gctggatgga ttgaccggac aactgtccga
    31621 acggcaagag cactatctgc aacgcgtgaa acataacgtg gatcggctga cgaggatcat
    31681 caatcaattg ctggactggt cccgcctgga tgtcggacgc atcgacatca aacccgagtc
    31741 gttgtctatc gatgcgtttg tccgtgatgt cgtggagagc tttcagaccc tggcggcaga
    31801 aaagtccatt acgttagacg tcctgccgtg tgagcaaccc cttgtcgtct atgctgatcg
    31861 agacaagctg gaacagattt tgtggaatct cgtcggcaac gccatcaagt tcactccgca
    31921 tgccggtcac gtctccgtgc aatgcactgc ccatgataca cattcggtcc aaatcaccgt
    31981 tgccgacacc gggtgtggaa ttgcccccga cgagttaccg ctggtgttta acgaattttc
    32041 caaagtggaa tcggccatgc cgacgtcgca aggcgcacag ctcggcctct ttattacgaa
    32101 aagcctcgtc accctgcacg gcggtcaaat gtgggtagaa agccgattgg gggcgggaac
    32161 gcagttctcc ttcaccttgc cgctctccgc cacgcaaccg gcgcaaccgt agattcacgc
    32221 ttcactcgaa gcgacgtctc ttccctgtat ccgttccgat aatagccgca ttgttttgaa
    32281 cagccctgac tggactgttc agaatgattc atctgtcggc ttgtcttgct tcgccggaga
    32341 atcaatgagt tgttcgtgcc tctcaggagc gtatcagcat ggcgtttgcg gcatgggtat
    32401 tgcggacatc aaccaacacg ggatgtttca ccagacagcg tggccccacc gtgcataccg
    32461 ccgtgctcat tgtcgatgac gatcgggata ttcgcgaatc cctcaccgac atgctgcggc
    32521 atgaaggtta tctcgtcgag agcgcggggt cagggaatga ggccctgtct catgccaaac
    32581 agcggcagta cggcgcggcc attctcgaca tccagctgcc tgacctcaac ggactctcgg
    32641 tgctgaaagt gctgatggag ttggacgcct cgctccccgt gattatcctg accgggaacg
    32701 caacctccga gaataccatt ggctcgctcg ccaagggcgc gtttgcctac ctgactaagc
    32761 cgtacaacgc gcaggagctg aaagccgtca ttcgtcgagc ggtttcagtg aaagggctcg
    32821 cggtacgcgc cgaacatgtc gagcaagccc tgcatgccag cgaggagcga ttccgcgcgc
    32881 tggtggaatc ggcaaccgac gccatcgtct tggctgacca ctacggccat atcatttggt
    32941 ggaatggggc ggccgaacgg atgttcggat attccaaacg tgaggcgctg ggacagtccc
    33001 tcgccatgct catgccggaa cgcttccgtt cggcccatga gacaggcatc atgcgcctga
    33061 ccacagggtc ggctgcgaaa ctgatcggga aaaccgtcga actggtagga ctgaccaaag
    33121 ccggggatga atttcccatc gaactgtcct tggcctcctg gcgtgccaag gagggcgcgt
    33181 tcttcagcgg catcatccgc aacattacgc ggcgcaaaaa ggcggaagaa tccgtcgtcc
    33241 ggctcagcca ccagaacgcc ttgattctcg acgccgcagg ggaaggaatt ttcggccttg
    33301 accttgaggg acgcacgacc ttcgtgaacg ccacggcggc acgaatgctg gagtggagtg
    33361 cggcagaatt gatcggcaaa ccccttgccc cactcctgta tagacttcat gacgaaggag
    33421 ccgagtatgt ccaggatccc ttatgtcttt ccgctgcagt acgcgacgga gcggtgcacc
    33481 gtgtacaaaa cgatgtgttc accagcaaaa gcggggtgtc attcccggtc caatacgtaa
    33541 ccagtccgat ccgtgaacag gatcgaccag tgggcgtcgt ggtggtgttt caagatatca
    33601 ccgcgcggaa gcagctggaa tgtttgcaac aggcacaact ctccatcagc catatcctcg
    33661 ctcagaccag cacgctcgaa gaagccgtcc cccaactgct ccgcgtgatc ggagaaatgg
    33721 gaccgtggga gatgacgatc ttctggcaca aagaaccgat gaacgaaaga ctggtctgtg
    33781 aaggcacgtg gatgcgatcc tcctgccgct gggatgaatt cgccacgatc tgccggaata
    33841 gcgaattccc tccggggatg gattttccgg gggtcgtctg gagcacgaaa ctgccactgt
    33901 ggattcccga tgcgttgaca gacgcccgct tcacccgcgc gccgaccgcc gccaggctcg
    33961 gaatacacgg ggcctgcctc atccccatcc gaaccgggac cgtcatccat ggcgtactgg
    34021 aactgtatgc cgacaagcct cgcctgcctg atcgccagct cattcacatc ttgaccgata
    34081 ccggcatgaa aatcggacag tttatcgacc gcgcgcgagc cgggtcggca ttacgcgcga
    34141 cccatcgaat gacgcagaat ttgctggcca gcctgccagg cgccatcttc ctttgcgccc
    34201 gtgacttcaa gatccaatat gccaataccc tggcgcatca ctacttcgcc gtcgggggag
    34261 cgtccttagt cgggaggccg ctgttcgaat gcctcgcgct cagtgaggcc gcgacgcaac
    34321 aattgatcaa agaatgggct gcgctcaatg ccgactcacc gcatcaggcc cccgaacgcg
    34381 aatgcgaagt cgcttcacga ctgtatcggt accgcttctt cccggtcgcc actccggaaa
    34441 gcgctcagta tcaggtaggc atcgtgctgt gggacgtcac cgacgacaaa catctgcagg
    34501 atcaactgat tcaatcggag aagcttacca gtctgggcac aatggtctcg gggatggcgc
    34561 acgagatcaa taatccggct caagccatac tcagcatggc cgaactgatt caggaagaaa
    34621 aagacccggc ggtgatcaag gaatttgccg ccgatatcgt cggctacgca cgccacgtct
    34681 ccaatgtcgt gcgggatttc gccggatacg ctcgttcggc tggccgggac ggcgaaacgg
    34741 aaatcgatgt ggcggagcgc ctcatggaag ccgtcaagat ggcccgccga ggtccgcatt
    34801 tcggctacgt cgatgtcgtc acgcgcttcg acgcccccac ttttctgcgc gcccgcaaga
    34861 gcgaactcga tcaggtcttc gtcaacttga tcaacaatgc cacgcaagcc atggacggga
    34921 aaggccgcct gaccttaggc gcggctcaag acggtgcgtg gattgatgtg acgatttcgg
    34981 ataccggccc gggcattccc gctgccgtgc ggcctcgcat ctttgatcca ttttttacta
    35041 cgaaggcgcc gggaaagggg acggggctgg gtctcagcat cgtgcataag atcgtgactc
    35101 ggcatgcagg gacgattacg gtggacagca ccgaagggga aggcaccacg tttaggctgc
    35161 gttttccggc cttgttgcag taaaggggaa acaagcacgg gtcgacgacg agcccgcgga
    35221 gggatttacg cgcgggaacg agaagagtag gcccgcttaa agacggaccg caatccgaag
    35281 taggcgaatg aatccttcag attggaatag agacgtttaa aggcgcccat gtcgggattg
    35341 gtcacatact cgagcgtgag cacaatccgc tcttcctgct cgccgagcgg cgtcacggca
    35401 tgccagagct tgtctccatt gaagatcacc agatcgccgg gcgctgtggc caattccata
    35461 tgtaccggct gtttacccgg cacatcttta aacagatcac agactaagcg gcattgtttg
    35521 gactgatcca gcaaccccag cagaatcgtg taccgcgcgc ctttatagta agaggtatcg
    35581 tagtggtatc cgatgtggtc gccggcttcc gtgtagtaat acaaggcaca ggaatgcgga
    35641 tcgttgtcgg ggcagagtag aagattgacc ctggtcaact gattgagcat ggctcgaaac
    35701 gcgtctgaac gatacaggtc catgaactgc ggggcctgct ccctcaccgt atagtagctg
    35761 acactgccgc ccttcttatg tcccggaatg taattgcgat tcaacccgga tttgagcacc
    35821 tgcgcctgtg acgccagctc ggcctcgaca aactcgcgcg gcagaaattg gggaatatac
    35881 agaaactcgt tctgctccca atagtcgcgc tccaaccggt cgagatccag ggccgaaatg
    35941 gcccgctcaa ccgcctctgt tacgctgctt gcacaggcct gcgtcatgcg tccctcactg
    36001 agtccggatt catcatggtt ccactgagga catcgtcttc cgatgaacaa aacgccactt
    36061 aaaaaaaagc aagttgcttg caggatggtc aaaaagtccg tccagtgagg ccgcagcgag
    36121 cagacaaccg aggcgtaccc tcgcggtacg ttgaggttgc ctgcgatgcg agaacgaagc
    36181 tggctgattt ttcaccatcc tgctagggtc ggctcagcac cggcgccctg acgatttcta
    36241 catcggccgg ggccttgaaa tcaaataggt cgtccttcaa tccgctgttc ggcttgagtt
    36301 ctgagaattc aaaggtggcg acgttgccac tgacctcatg cagtgcaatg gtcttgagga
    36361 agtaggtttt aggctgcacc tcgatcacga ttttttgaat cgctcgttcc ggttcggcac
    36421 gtccctgttt cggagtcagc gataccagga gaataccccc ggcgccccgc tccttattcg
    36481 gcatcggttc gatatcgaac tcctcatcca gcttggcaac accctgcaag agctgcagcg
    36541 gcgcttgtga cgcggccata taggtgagct taccgaccag gacctgctta tgctcgggca
    36601 catacatctt cacatcgtct ttgttgacgt agatttcttc cgtggccggt tcaatatagt
    36661 cccaccgcaa cttgccgggc ttcttgatgt aaaagtgacc ggtcgaaatc accggcgtgg
    36721 aaaatccttc aatcctggtt ttttgcgtga atgacgcctg caaatccttg gtcttttcat
    36781 atcgcgactg aatgttcttc accacctcct gcacttcgt
//
LOCUS       DAOQEW010000013        35512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_246_length_35512_cov_7.986745, whole genome shotgun sequence.
ACCESSION   DAOQEW010000013 DAOQEW010000000
VERSION     DAOQEW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 35512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_35512_cov_7.986745"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLS35_02780"
     CDS             complement(<1..922)
                     /locus_tag="PLS35_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNI17606.1"
                     /translation="MRMTVCVITLLLVTGCAVGPDYSRPELQMPTGFRMAVSDQETQS
                     FANLPWWDLLQDEELRRLIRVALAENKDLQRAVASVEEFQSRLLISKMDFAPKADVTA
                     NAPSFGRKANFLFPGFPNPFNYYLQGNLSWELDIWGRVRRSNEAARGDLLAREEARRA
                     VVLQLVSGVAEAYFELLQFDMQLGVAQRTLKSWEESVRIAEARLRQGMISRIDADQFE
                     AERANAAARAAELTRQMVQKENQLSILLGRPPGQVARGRSLTEQVLPPEVPAGLPSEL
                     LQRRPDLVQAEQELAAATARIGVAKADRFPK"
     gene            complement(935..1240)
                     /locus_tag="PLS35_02785"
     CDS             complement(935..1240)
                     /locus_tag="PLS35_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17607.1"
                     /translation="MLKITSEQTGDSARLTLEGSLSGPWVTELERTWQTVRLSGIFAP
                     VVELVGITFISEDGKALLARMWREGAALVAHGCCTRHIVGEITGAAPVAGPGQCDPA"
     gene            complement(1252..4335)
                     /locus_tag="PLS35_02790"
     CDS             complement(1252..4335)
                     /locus_tag="PLS35_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI17608.1"
                     /translation="MRSFTDVFIKHPVLAVVINLVIVLVGWRALTTLPVQQYPKIESS
                     SVIITTVYYGASAETVRGFLTTPIERVVSAISGVDYLESTSRAGISTVTVHLKLNHSS
                     TAALAEVTARLQQVRSELPAEAEPPAVEVQRADRPYASFYLSFTSPERTVPALTDWLL
                     RSLQPQFATLTGVQRVTIEGGRQIAMRVWIDPDRLAAYNLSPGDVQAALRRNNYLAAV
                     GRTKGNLVQINLLANTDLRSTEEFEDLIVTDRGGAIVRLRDVARVENGAEEAEMVAKY
                     NRTEGVYLGIWPLVGSNEIEVAHRLREEMDRIRPTLPKDIDMQLVWDGTMFMRSALEE
                     ITKTLGETVLIVATVVFLFMGSVRTALVPLVAMPVSLVGAAIFMYAFGFSLNLLTILA
                     IVLSVGLVVDDAIVVVENVERHVREGKSRIQAALLGSRELVGPIIAMTITLAAVYAPI
                     GFQGGLTGSLFLEFAMTLAAAVVLSGVVAITLSPVMSSYFVHPEGKEGRLTKMVNRVF
                     DATRAGYGRLLDGALEMRWAIVVVALLVMVAAWPLYQFSRQELAPVEDQSHISLFLEA
                     APDSTVAATNRDSLKVIDALRAFPEARFTWSLTSSWGGFGGLVAKDWRERSRSTEMMY
                     GQVYGAVSQIPGLRVFPRLDPPLPTPGQYDVELILESQLPAEQLMETTAAVLGAGWQS
                     GKFLYVDTDLKLDLPEARVLIDRERLADLGFDLARVGQELGTLLGGAYVNRFNFYDRS
                     YKVIPQIGDKDRATLDPLLDLKIKTPGGQLVPVSTFTRIETSTAPRTLNRFQQRNAVR
                     IFGGVKPGVTKDEALRVLETAAAAAAGSRATLDYAGESRQIRHEGSALIVTLGFAVVL
                     IYLVLAAQFQSFRDPLIVLLGSVPLAISGALVFSFLNLTTINIYSQVGLITLVGLIAK
                     NGILIVEFANTLQARGLSKTAALREAAMTRLRPVLMTSAATVFGHLPLVLVSGPGAAA
                     RNSIGMVLVTGMTVGTLFTLFVVPVFYSLIATRHQPAEELEPAEEPGLQLAGAKG"
     gene            complement(4353..5480)
                     /locus_tag="PLS35_02795"
     CDS             complement(4353..5480)
                     /locus_tag="PLS35_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI17609.1"
                     /translation="MNRRGWIGSSLLMLTVIGIGVGLAVWKYGSIQDQAAASTNQPEP
                     MEVVTLAVARAVDHHQTATSIGTVLALRSITLRNELAGTVSQVRFTPGQIVEAGTVLV
                     ALDVSVEEADLRAQQAQAVLARTVLDRRRALTQDLAAAQEEVDRAGADLDVVQAQIAR
                     TKALIARKTIRAPFRARVGLADVHPGQYLNEGTQLTTLQGIDEAVHVDFAVPQHVAAG
                     LRHGDLVDVYPAGDTSVLQAKIVALDARIDPATRNAMVRARIDRSPSMPTPGAAVRIK
                     VRVGAPRSAVAIPVNAVRKGPGGDQVFVINSDGQGKSRAQVRHVESGAMSGDDIIVYA
                     GLAAGEQVAASGAFKLRDGVLVVAAGGPVAKSDATPSPEQP"
     gene            complement(5586..6113)
                     /locus_tag="PLS35_02800"
     CDS             complement(5586..6113)
                     /locus_tag="PLS35_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17610.1"
                     /translation="MEFTMPTTEQAHWGLRAMKTVALADGVVDESERHMLEAVQRVFG
                     THHDVDGLQPLAPEELAAGLPDQQIRRQLVNGLIVMSLIDQDVKRAEAELVERFASAL
                     QVTLPEVTNLRHVVKKELFHLRLDLVRRSMIAPLFRQMPTGRQSEFGTRQHPALPRKK
                     WRELWQKNRWFTAMV"
     gene            complement(6149..7102)
                     /locus_tag="PLS35_02805"
     CDS             complement(6149..7102)
                     /locus_tag="PLS35_02805"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNI17611.1"
                     /translation="MESVTELLEQHGAWVLFGGVLAEQLGLPFPALPLLVAAGVLVGT
                     GHLSWSGALIAALSATLLADIVWFLAGRWRGRPVLTLLCRIALEPDACVRRTEDFFRK
                     HGVQSLLVAKFIPGLSTIAPPLAGIVGLGLPVFLLYDALGAAVWAGSGLGFGLLFTEE
                     VALALTYSEQVVPALLLAAVVLLPAYIGWKAWHVRRQLQAVPRMTVADLLDKLAAPDP
                     PLLIDVRTRLAVEAEPGLPNALQMSLEDLARRHGELPRSRDLVLYCACPADAASAQGV
                     MLLQRKGFTRVWPLAGGLHAWRAIVDGPEPVPMIQVERLAV"
     gene            complement(7340..9961)
                     /locus_tag="PLS35_02810"
     CDS             complement(7340..9961)
                     /locus_tag="PLS35_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI17612.1"
                     /translation="MAPYTMPMHDMMPSSVEPMMADRGAALLQVAEVIAAHHDLPSLF
                     QDLAQCLPVVAPFDFVGLVLHDAAKHVMQVHVLETAEAKRVTRRLDGLEIPMKDSASG
                     WVWEHQQPILIPSLAAESRFQVGMDALRGIGVQSVCFFPLTTAMRRLGAIGFGCLKPF
                     AFDEGTVSFLNQVAKLVAVAVDNVLHHQDLARDRDRLRLLLEVTESIASHHDQSLLLA
                     DLAKRLPQVVPFDFMNVVRHDPSRDVMRLWLLVTSEQSTIEPGLETPVDESPGGLVWK
                     TQEPLTVDDVEREPRFPALMTLFRANGVRSFCVVPLTTAHRRLGAMGFGSLRPRVYLD
                     SEIAFMKQVAQQVAVALDNALNSESTLAYQSQLTRERDRQRLLLEVNNAVVSHLDLDQ
                     LFPAVSACLRRVIQHDGSSLLLCDEETGRWRIHVLDFDRNENFIEEGRIEETTQSPSC
                     LAITTGKAALFNERDLLAMGGASPCAQDLLDRGIKSFCSVPLLSRNRSIGALNVGRRR
                     DAGFDPEDVQLLGQVAQQIAIAVENALAFREIAALKDKLTKEKVYLEEEIQTDYNFEE
                     IVGESRALKRVLNQVQTVAATDSTVLILGETGSGKELIARALHNLSARRERTFVKLNC
                     AAIPTGLLESELFGHEKGAFTGAIATKIGRFELADRGTIFLDEVGEIPLELQVKLLRV
                     LQEQEFERLGSTRTMRVNVRVIAATNRDLSHMVDAQQFRSDLYYRLKVFPVTVPPLRE
                     RVEDIPVLVRHFVQKFASRMKKHIETVPTDAMRALQAYGWPGNVRELENFVERAVILS
                     SGSELFVPTAELKRPVSPSNGSATTLEDAERDHIIKALRDTHWVIGGSSGAAARLGMK
                     RTTLQSKMQKLGIVRPQ"
     gene            complement(10016..10843)
                     /locus_tag="PLS35_02815"
     CDS             complement(10016..10843)
                     /locus_tag="PLS35_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17613.1"
                     /translation="MKSHILLSLCLAVLAATPLTGQAQAAQPGNVTITATDYGFKSPE
                     QVPSGLTAIEVINEGAEVHHAQLVRLAPGKTPADFAAAMKADPEHPPIWATFVGGPNA
                     VVPGDRATAVMDLQPGQHLALCLVPNKAGVAHVAMGMARPFMVTETIGTRIAEPKPDV
                     VITARDFQFVLSKPITAGSHTIQLLNEGSQTHEVVLVKLAPAATAKDFIAAFEPGAVG
                     PPPGRPLGGIVGIEQGSRGLFTAMFDPGPYALICFFPDKKTGAPHFAQGMTWEFDVR"
     gene            10985..11440
                     /locus_tag="PLS35_02820"
     CDS             10985..11440
                     /locus_tag="PLS35_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17614.1"
                     /translation="MIPPAIVDLLQTGVSVNVGTRDKNFMPECTRGWGIWVAANRTTV
                     TLLLTEPASARTLANLRDNGLMAITCSRPTDHVTCQLKGSVTQIRQATPQDYERQQQW
                     RHAFLGELIAVGVPAEQTDALIMQPAVAIEMQVTGVFAQTPGPGAGEQV"
     gene            11444..12829
                     /locus_tag="PLS35_02825"
     CDS             11444..12829
                     /locus_tag="PLS35_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNI17615.1"
                     /translation="MGTAALALKDLWPCFEGIIPATIATCAKDGTPNISYVSQVHYVD
                     PEHVAISFQFFNKTRKNIAEHPFASVMVLDPRAMQAYRLSLTYERSESSGPLFQAMSL
                     QLQAIASHAGMSDVFRLRAADIYAVRHIERVDGFTSWPVPAHDERPPSLFIDPNLHAL
                     RVVAEQLNRAECLGELLETALRCLDEFFGLHHTMLLLVDEKRRTLQTIASRGYDENGV
                     GSEVGFGEGLIGMVAAAKLPLRLSGLDYTLRYARAVRDRAQALSGPESLCREIPLPGL
                     SNASTQIAVPLIIRHRCLGVLVAESLEPLTLLIREEAVMAIVAGHLAAGIEQSAQEAD
                     DSVAVEAPAAAAPPLHSPLSHRSFCFYQADDCIFVDGEYLIKNVPGRILWRILKQHQD
                     EHRVEFTNRELRMDSWLGLPEWKDNFETRLILLRKRLEQKCPDVRLVPRGRGRFALET
                     SVKIILSEKPA"
     gene            complement(12863..13372)
                     /locus_tag="PLS35_02830"
     CDS             complement(12863..13372)
                     /locus_tag="PLS35_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HNI17616.1"
                     /translation="MPVPILRRTVDLSGFPDLVVIYLGMRVNRWTGLKTLFGFGPQIA
                     ASVDAQPEGLLLHESLLWSLMPPHVGMRQYWRDFDALERWARSDPHRQWWQQFLRDTG
                     GTGFWHEAYFMRGGMEAVYNDILQDIGFLRFAPIRPAKGAQFTARSRAGRTGESALPP
                     AVLEEDLDR"
     gene            complement(13424..13750)
                     /locus_tag="PLS35_02835"
     CDS             complement(13424..13750)
                     /locus_tag="PLS35_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17617.1"
                     /translation="MVHRNGRIGLGLVVLLWCVLAGGTLAVPLTRAEEPTIEFNSPDV
                     LRQILEQQVGKRVKVRLLSGQDVDGKVAKVGTQAVALTELSGMEFFDATIRLDQVAAV
                     IVRRGK"
     gene            14016..14564
                     /locus_tag="PLS35_02840"
     CDS             14016..14564
                     /locus_tag="PLS35_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNI17618.1"
                     /translation="MGWIILAVLVALVLVVIGMYNSLVRLRAAAESAWADIDVQLKRR
                     YDLIPNLVETVKAYAAHEKGALEAVITARAKAMSAQGPEAKAVAENQLSQSLKSLFAL
                     AEAYPQLRAVEAFNQLQGSLNQVEDALQNARRYYNAVVRDYNAKRQEVPTNFIAGWFN
                     FAGRQFFELTETTQREPPRVQF"
     gene            14566..16338
                     /locus_tag="PLS35_02845"
     CDS             14566..16338
                     /locus_tag="PLS35_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNI17619.1"
                     /translation="MDRAGDPIVKPRRRPHFVHVLSSLIFLCLIESVSLLPADARSFV
                     LSRFDVELQVLSGGELLVTETVSPRFEGAWNGIERLIPVEYRTPQGFNYTLFLDRISV
                     TDEQGTPLKFESSRERHYRNLRIWIPGAADATRTFVLKYLVRNGLKFFEDHDELYWNV
                     TGDEWDVPIEQASVRILLPPQATGVRAQAFTGAYGAREQAATVTILGSGIEMRMTRPL
                     GFREGLTAVVGWDKGLVAAPTVVEQTRMTLVSNWPLGIPLLAFLVMYRLWSTRGKDPR
                     LRPIAVMYEPPDQLTPAEAGTLVDESPDTRDITATVVDLAVRGYVRISERKAEHLFGL
                     WSSMDYAFQRTKPRQEWAALPSFERLLLEALFKDSEVDEILLSSLENRFYKSLPGIHD
                     AIFQSLQKRTYYSQRPDRVKQGYLIGGIAFGMLLTIGLGILADRWGLAPLTVFVAGLL
                     SGMIVIGFGRIMPARTQQGTRALEGVLGFEEFLTRVEADRFDRVIKTPELFEKFLPYA
                     MALGVEKNWAAAFETIVMTAPAWYQGSDLTQFNTRGFTSRMGDLASRTGSTMTSAPRS
                     SGGSGFSSGGSSGGGFGGGGGRGF"
     gene            complement(16370..16816)
                     /locus_tag="PLS35_02850"
     CDS             complement(16370..16816)
                     /locus_tag="PLS35_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HNI17620.1"
                     /translation="MRYVIAIVVMCLSVATTVQYADARDRNGVFKASELIGMKVQGTD
                     GKNLGDIKDLVINPDDGAIDYAVLDFGGFLGIKDKYFAIPWDALQVASGKDKILIDAT
                     KRDLKNAPGFDKNHWPDFGDLAQTTVIYEFYGVPAPNTSGQHKATR"
     gene            complement(16990..17637)
                     /gene="pdeM"
                     /locus_tag="PLS35_02855"
     CDS             complement(16990..17637)
                     /gene="pdeM"
                     /locus_tag="PLS35_02855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HNI17621.1"
                     /translation="MRIRIRDECVALLPERAMFWPTHSLLVVADCHLGKAETFQQQGL
                     WLPSQSGQQDLTRLSALVLSVDAQHVLFLGDLVHSLSGVTDAVVAEFARWLHEFSGSV
                     RVVLGNHDGGLARRWPKEWVKAERCDQVTIAPFRFKHQPIEGTVEADTFYWVGHVHPM
                     IQLAQGPDRVRLPAFVINDRQGVLPAFSSVSGGYDVPLRPDERRFAIGGDRIYEL"
     gene            complement(17638..20199)
                     /locus_tag="PLS35_02860"
     CDS             complement(17638..20199)
                     /locus_tag="PLS35_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HNI17622.1"
                     /translation="MSSSAKVLRGIQRLNRMGPLRPRPDAEEQARARRKAWPTLSREE
                     AWKQWNEWFDRQGWSPFPFQVEAWQAFDAGRSGLVAVPTGSGKTYAAVGGPLLSLIAR
                     PIAGHQPPLTILYITPLKALARDIAQAIQQPLDDLGWPIDVDLRTGDTTATARRKFKE
                     RLPHVLVTTPESLAILLTDQGWKDRMRAVQAIILDEWHELLGTKRGSLLELTLARLRA
                     MAAGARVWALSATIANLEEAASIAVGSGLEPTIIRADIPRHIRIHCLLPESIQRYPWF
                     GYSGLSLLPEVVRQINPDQSTLLFTNTRSHAERWYQAILEARPEWSTVMALHHGSLDQ
                     DERQRVEDGIKSGALRLVVATSSLDLGVDFPPVENVIQIGSAKSIARAVQRAGRAFHR
                     PGEDTLVQLCPTNVMEILEASALRQAMLGGVLEERTPLDKPLDVLVQCLLNASFNEGF
                     DPADLCRQVRSAVSFRTVTDDEWSWALAFIRSGGALSSYPEFRKVEVVEGRYRFVSTE
                     AARRHKMNIGTILSDSGVTVQVRGGARLGMIDETFVAKLRPGDVIQFGGRTVQFLQMR
                     DMTAYVKPARQRSPVAAVWSGTILPLSVQLSFYLRREVDRLRQAVEGFNSRSPEIDAL
                     LPIARLQSSRSRIPSTAQTLIETCRTAEGHHVFVYTWEGWSVNEGLGHLVAYRLSRLR
                     PNTIAVSATNYGFELLSTEPLGSDREIGQALTTLDGLEEDIHASQNFPELARRAFREI
                     ARVAGLIYQGTPSARKTARHLQMSASLLFDVFRQYEPSHPLLAQAFREVHDRQLQIDR
                     LRGLMRRMGEQELLIVPIPRLTPFAFPLYVERIKSRLSTEKLEQRVARLQREVFR"
     gene            complement(20196..21923)
                     /locus_tag="PLS35_02865"
     CDS             complement(20196..21923)
                     /locus_tag="PLS35_02865"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNI17623.1"
                     /translation="MQAFAILFEQLDQTTATNEKVRIMTEFFRHQDPDTSAWVLFFLS
                     GQRPKQIVGSTKLRRWAQEKVGYPDWLMEESYAAVGDTAELIALVLAAGEGAVKHAPL
                     SRLSLGEWMVQRILPLAGMDDQARRSNILAWWEELETNETFILNKLLTGSLRVGVSET
                     LVYRAVAAAFSLSPAVIASRLVGTWQPSAEFFLRLSAPAEETQTGQRSDETLLPLPFC
                     LAAPMEGDPEELGDISNWQCEWKWDGIRCQVVKSGSRLEIWSRGEERITGSFPDLSRL
                     LSPVSGDWVIDGEIVAGDWHRPDAFQSLQRRLNRKKPSLALIASSPVSFLAYDLLQAD
                     GRSWRHHPLAERQAMLERTLHPYIGERLGISPLLALSSWEQARLLRETSRVEGAEGLM
                     LKAKDSEYVTGRKRGVWFKWKIDPLAIDAVLTAAQPGTGRRASLYTDYTFAVWAGDSL
                     ITVAKAYSGLTDEEIRELDGWIRKHTRERFGPVRMVEARRVFEIGFEGISQSDRHKSG
                     FALRFPRILRERTDKRVEDADHVATLETLLASFGSSAGRPGRARARAESLPGQEQLQF
                     ELGAGDVGS"
     gene            complement(21923..22915)
                     /locus_tag="PLS35_02870"
     CDS             complement(21923..22915)
                     /locus_tag="PLS35_02870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HNI17624.1"
                     /translation="MSDLIRLQPEGLVGPGNRFHIDAWGQAAVNLITHAHGDHARTGS
                     AEYWCAEPCAPILRHRLGPDLRIRAVPYRKPVRLDDCWISFHPAGHILGSAQIRVEQG
                     SRVWVVTGDYKRDADPTCELFEPVPCDTMITEASFGLPIYRWASTRSVTGRIVDWWQR
                     CREEGVTALLFCYALGKAQRILAELTHFTQEEVLLHGAVAALTQIYREQGVSMVPTRL
                     VSDAEGPTEGRLILAPPSAHRSPWMKRFRAVSTAFASGWMAVRGIRRRRGYHRGFVLS
                     DHADWKSLLDTVEESRATRVLVTHGTSDTLARYLRDVKGAKAEALTTHFGEGED"
     gene            complement(23215..24237)
                     /locus_tag="PLS35_02875"
     CDS             complement(23215..24237)
                     /locus_tag="PLS35_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17625.1"
                     /translation="MLKPEPAGGPTPQLFFQTANAYQRSQALKAAVELDLFTAIGLGH
                     DQVPALAQRCGAAERGVRILADYLTIQGFLTKEGDRYKLTPDSAVFLDRRSPAYMGSV
                     LGFLHAPKFIAAFEHLTEAVRKGGTVAGEAGTVAPEHPLWVDFARSMIPMMAKPAEEI
                     GIFVRQSQPNVKKVLDIAAGHGLFGVEIASRCPQAEIVALDWPNVLTVARELAQQAGI
                     ASRYRTIAGDAFQQEFGECYDLVLLTNFLHHFPVQTCESLLRKIHRSLAPGGRVITLE
                     FVPDENRVTPPPVAEFSLIMLATTPEGDAYTFREYEQMFAAAGFSRTELHPVAGSIEQ
                     VLVSTL"
     gene            complement(24397..27693)
                     /locus_tag="PLS35_02880"
     CDS             complement(24397..27693)
                     /locus_tag="PLS35_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17626.1"
                     /translation="MDLVRATHSLHKPIRAKTRAFDHHVETQGPPAQKPPSIPAGLDR
                     TDWMCAALFSLAVAIACLLIARAMPSFLFQSMDFWFEADTLREISNMTRVHDDHYRTS
                     VHPLFSLLTFVPVYLIKHGLSVSPLRAVLLVGSLVGGLWGGTLYVLFRLLGCRKPDAI
                     VLTLLGLVSASALFWLPVPTSYSWGSLSIMLALVLLLYTERRPIGASVYVVASALTLS
                     FTVTNWMAGLLVSLARWPWKQAVQLSINALCLVVILWGVQKVIFPSAAFFIGQREEAS
                     FMNHPQAGGITNVASSIVFHSLVAPTVRFLKDDAYAQANADAFRLSERLTFQFSTPGS
                     GGPLGLVAVILWSGLLLNGLWRLVTLDHHLRFRLVLGSMLAFEVLLHMLYGEETFVYS
                     LNFMPLLLAVAACGALAPGRRIVTGVAAVLVVCVALNNWQQFQAATHTATQFTPQREL
                     MTAMMEKDPERPWPRSVGHVPLAVPGSPEGGTAYHEPGGDFSPQLPSFGVSLWLCDAT
                     GAPVVVSQTVPIHEITQEFAPSAHPTIPAITTKTPYYQATWTRLDASRWELRFKNETN
                     HVPVIVLRSVGPAGGPVTALNQEEGKVTVNHRWAVTATPAPDRIAMGDENTAGWMTEQ
                     SAVTSWSGEAGWGYARLQFSLGTTKPGEEIRVVLSDLRPPVEIEGYYKQAPKRARLAL
                     PDPRLQASMNAQITHLMMSLVDDETRPGDPAVFYRAWHRQGAYITAALARAGEPRVSR
                     VLSQFLAMHDFAGGSGPEADAPGLTIWALTESADYIGDKVHDEWLWPHILRKARRIEE
                     MLTAKTPLVEPYTIPAPHDFKHLRQTRTAVLAQPAREGLIVGRVGEEWPSLYVNAVSY
                     RGLVAAAEFAERLGKRQQAARWRTQAQGLLESWQRQSTHDSMQGSASPVSLRALADPS
                     SRRHQLKQLLTTYRPTADHTTSAGQLSQAHQALRQGKPEAVWTVLHRLWGEQASPGLY
                     TWEAALSSKDEVADGWQYARGWHNELAVTPDYETAALLLLLQQDMLAYLDEQAAEPTV
                     VIGAGITAGWLSQPMAVSNLALPGGSITWQWDGHAMRVTVRGPERAVRLGTAFPPGTS
                     LIVTHKPAIH"
     gene            complement(28102..28308)
                     /locus_tag="PLS35_02885"
     CDS             complement(28102..28308)
                     /locus_tag="PLS35_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17627.1"
                     /translation="MDVAQVLIAIVGFGAAVTTYCLMRFPHMRREPFRQYRGCSVLVS
                     SLAALVVLCLALVVLIAQYAWHAR"
     gene            28424..29005
                     /locus_tag="PLS35_02890"
     CDS             28424..29005
                     /locus_tag="PLS35_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI17628.1"
                     /translation="MTAIVTIGTKRLRLRPFQTTDAEAVHRLAGSQDVAAGTFLPHPM
                     DRQAAQDWVTERLKDQAAGTGVTFAITLLENGELIGSIGMELMLVHEQGRLTYWLGRD
                     YWNQGYGTEAVTALVEYGFNSLSLHRIYAPHFHTNPASGRVLQKVGMTYEGRLREHYL
                     RFGQRIDVELYGMIRNEFLGLKVGEKGVADDHG"
     gene            29160..29804
                     /locus_tag="PLS35_02895"
     CDS             29160..29804
                     /locus_tag="PLS35_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17629.1"
                     /translation="MWQSRNQIQIPDNSNGTRFGLTDIQGNGPNVQRRVELTWNLSHQ
                     HSLRFVYAPLGFSGTGSFGSPVRFSGGTFAPGVPIDSDYKFDSYRLTYRYLVHESERW
                     RWRIGATAFIRDARVELRQQGRVASDSNVGFVPLLSTSLEYDIAPRWTALLDFDGLVS
                     TQGRAIDAALKVRYDLTDHWFMTAGYRVFEGGVDNDRYAFGWYNFALVSVGVRF"
     gene            complement(29784..31145)
                     /locus_tag="PLS35_02900"
     CDS             complement(29784..31145)
                     /locus_tag="PLS35_02900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNI17630.1"
                     /translation="MGISPSLRGMSVIVAGAGLAGLTAAYELQRRGAQVTVVEARDRV
                     GGRVWTRRDGFAEGQHAEGGGDMIDPDQESIRRLAKDLGLTLSPILRGGFAFARQAAR
                     GPVITQASKAGDLWAQLAQAAEPWVRAHRLNERRWDGPIARQLAEQSVADWLDSIGAN
                     QQLRARLNGMRGFFLADPEHLSLLALVDQLSAESSALDHFYRIKGGNDRLATALADTL
                     NEPIQLKTIVCAVAQRRGQRRVTVRRGGQQDHITADAVVLALPATIVRRLAFSPPLPS
                     PQRKAFQDLQYGPATKTLLQFDRRFWRHQGAPLAYGTDLPIGAMWDGNEEQRGRSGIL
                     TLLAGGSASRDTQRLLAHGGAEAVAEQLKWLGATKAVLCASHHVSWEDDRFVRGGYAV
                     FQPGYDPEQRAWLSRPHGRILFAGEHTSLRWQGYMNGAVESGLRAALEVECLLGRLKS
                     NAH"
     gene            31317..31964
                     /locus_tag="PLS35_02905"
     CDS             31317..31964
                     /locus_tag="PLS35_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17631.1"
                     /translation="MNADHPSSPSSPDMLVPEPSMNSTPADERSFHIRLAVFASVVII
                     AVVGAFVGYPTWQEYVSQQEAKLELRAYVNVRALGPPALKEGVSPRVRETIQNAGRTP
                     AYNDAIQAFITVADYPLIKNFERVTCPPDDSQPDKRKWFMGKVPRTETVRETPFTAEE
                     ITAIQTTKSALYYHGTVCYRDIFHESHRTDFCMFWKWDAGRLSPSLSCDQGNKTD"
     gene            complement(32001..32471)
                     /locus_tag="PLS35_02910"
     CDS             complement(32001..32471)
                     /locus_tag="PLS35_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNI17632.1"
                     /translation="MTPWKRQGYALKSGEGPSIEFRGTKMTVKLSGASSEESHSLIEM
                     VHPSGVGPALHIHPTGAEAFYVLEGHYTIHCGEQQYEAAAGDFVYVPKGLPHLYQSGV
                     NGGKVLVLSPAGLERYFSEVAELLRVGPVTWDVEQAIAAQYGQEFLDGLRHWGQ"
     gene            complement(32598..32942)
                     /locus_tag="PLS35_02915"
     CDS             complement(32598..32942)
                     /locus_tag="PLS35_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17633.1"
                     /translation="MENKSDAEILAVADPIMDNLMEASTAIDHARHVQDFTDRLKAIV
                     TKDYLERVCRQYQAEKGTFGARTPVAVFRRPGSIAIVWKQHFIDSPGDYVAEMVLVQQ
                     GGRYLVDHVFVL"
     gene            complement(33077..33541)
                     /locus_tag="PLS35_02920"
     CDS             complement(33077..33541)
                     /locus_tag="PLS35_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNI17634.1"
                     /translation="MPKKLSAGIVLYRRIGGGLQVFLVHPGGPFWAKKDSGAWSIPKG
                     EYIEGEEPETVARREFQEETGGELTGRLQALTPLKQPSGKVISAWAVEGDIDPAELKS
                     NSFYLEWPPRSGTIQQFPEVDRGAWFDLPTAQEKMLVGQRPFLEELVRFVKE"
     gene            33608..34267
                     /locus_tag="PLS35_02925"
     CDS             33608..34267
                     /locus_tag="PLS35_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17635.1"
                     /translation="MKHHTEPDGTLVVTIDSRFIVGMLTGAALLCAVPVFRSWLIGGA
                     TAHDATPLIGSAMFLVGAFFSYERNRFVFDPHLKEVRWSRRSLMAKDEGRMPFSCLRS
                     LTVQAAIGSTKTAPLVRLALVTDEREIPLSRAYAGGMRREYEELAITIRRLMQMNAER
                     TNFLLDSLTAALAQGRKLDAIKLVRMTKDMTLEEARQFVEQLAVSPTAGSRSNDSPPS
                     S"
     gene            complement(34299..>35512)
                     /locus_tag="PLS35_02930"
     CDS             complement(34299..>35512)
                     /locus_tag="PLS35_02930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNI17636.1"
                     /translation="CDVLSDDQDVVERHAVVLWENGQLICRAVQGIVYLDGHKTMESV
                     LTLQQQIRMGRSLWQLNGPTPGRGSGGWIEDLSDRISEVAGVERLQGFNARDMFSDVL
                     RKRTDEEIEEHFTAGTLTTTPPIEKVITGWPKPWVFFKTFILSAVVYLAFVFAMKEFN
                     NANLVPGLIMTGAFLIPSSLLIFFFEMNVLRNVSLYQVVKMVMFGGVTSLILSLFLFK
                     WTDLDSWMGAMSAGVIEETGKALALLLIINNQKYRTALNGLLFGAAVGTGFSAFESAG
                     YALMAGLSGGQSAMLHSITVRGLLSMLGLHIVWTSMVGAALWRIRGNRSFTIEMLYDK
                     RFLRIFGLAVALHMFWNAPFNLPFYLKYIALGFIAWVVNLALVQVGLREVKALQEAQA
                     SEASDSRLRTA"
BASE COUNT         7366 a        11438 c        10126 g         6582 t
ORIGIN      
        1 gcttcgggaa tcgatccgcc ttggcgaccc cgatcctcgc cgtggccgcc gccaattcct
       61 gttcggcttg caccaggtcg ggccggcgct ggagcaattc ggacggcaga ccggccggca
      121 cctccggcgg caacacctgc tccgtcaaag aacggccccg cgccacttgc cccggcggtc
      181 gcccaagcag gatgctgagc tggttctcct tctgcaccat ctgcctggtc aattccgccg
      241 cgcgagcggc cgcattcgcg cgctccgcct cgaactgatc ggcgtcgata cgtgaaatca
      301 tcccctgacg caatcgggcc tcggcaatcc ggaccgattc ctcccacgac ttcaaggtgc
      361 gctgcgccac acccagctgc atgtcaaact gcaacaattc aaagtaggcc tccgccaccc
      421 cgctcaccaa ctgcagcacc accgcacgcc gggcttcttc gcgggcgagc agatctcctc
      481 tcgcggcttc gttcgaccgc cgaacccgtc cccagatatc caattcccag gacagatttc
      541 cttggagata gtaattgaac ggattgggaa atcctggaaa caggaaattg gccttacggc
      601 cgaacgacgg cgcattggcc gtgacatcgg ccttcggagc gaaatccatc ttcgagatga
      661 gcaggcgcga ctggaattct tccacggacg ccactgcgcg ctgcagatcc ttgttctccg
      721 ccagcgcgac gcgaatgagc cggcgcagtt cttcgtcctg caacagatcc caccagggaa
      781 gattggcaaa ggactgtgtt tcctgatcag aaaccgccat ccggaacccg gtcggcatct
      841 gaagctcggg acgtgaatag tcaggaccga cggcacagcc ggtcacgagg agcagagtga
      901 tgacacacac agtcatacgc atgatgaacc tattctatgc agggtcgcac tggccggggc
      961 ccgccaccgg ggctgctccg gtaatttcac cgacaatgtg cctggtacag cagccatggg
     1021 cgaccaaggc cgccccttcc cgccacatcc tggccaggag ggccttgccg tcttccgaga
     1081 tgaacgtgat cccgaccagt tctacgacgg gcgcaaatat gccggacagc cgcacggtct
     1141 gccaggttcg ctccaattcc gtcacccagg gcccgctcaa actgccctcc agggtcagcc
     1201 gggcggaatc tccagtctgc tccgatgtaa ttttcaacat aatgagtcca ttcacccctt
     1261 tgcccccgcg agttgcagac ccggttcctc ggccggttcc aactcctcgg ccggttggtg
     1321 gcgcgtggcg atcaacgaat agaacaccgg caccacgaac agcgtaaaca gcgtgccgac
     1381 cgtcatgccc gtgaccagca ccatgccgat gctgttccgc gctgccgcgc ccggccccga
     1441 caccagcacc agcggcagat ggccgaacac cgtcgccgcc gaggtcatca acacggggcg
     1501 caaccgcgtc atggccgcct cacgcaacgc cgccgtcttc gacaggccac gcgcctgcaa
     1561 ggtgttggcg aattccacga tcagaatccc gttcttcgca atgaggccca ccagcgtgat
     1621 caaaccgacc tgcgaataga tgttgatcgt cgtgaggttc aggaaactaa agacgagcgc
     1681 gccggagata gccaacggca ccgagcccaa cagcacgatc aacggatcac gaaaactctg
     1741 gaactgagca gccagcacca gatagatcag caccacggcg aatcccagcg tcacgatgag
     1801 ggccgacccc tcatgccgga tctggcgcga ttcgcccgca taatcgagag tcgcgcgaga
     1861 accggcggcg gccgcggcag ccgtttccag cacacgcaac gcttcgtcct tcgtcacgcc
     1921 cggcttcact ccgccgaaga tgcggaccgc attccgttgc tgaaaccgat tcagcgtccg
     1981 aggggccgtg ctggtctcga tgcgggtgaa ggtcgacacc gggaccagct gccccccggg
     2041 cgtcttgatc ttcaagtcca gcagcggatc cagcgtggct cgatccttgt cgccgatctg
     2101 cggaatgacc ttgtagctcc ggtcatagaa attgaagcgg ttcacgtagg cgccaccgag
     2161 cagcgtgccc agttcctgcc cgacacgggc caggtcgaac cccaggtcgg ccagccgctc
     2221 acgatcgatg agcacgcgcg cctctggcaa atcgagcttg aggtccgtat cgacatacag
     2281 aaattttccg ctctgccatc cggcaccgag cacggccgcc gttgtttcca tcaactgctc
     2341 ggcaggcaac tgactttcca gaatcaattc cacgtcatac tgccccggcg tcggcagggg
     2401 cggatcgagc cgcggaaaga cgcgcaaccc aggaatttgc gacaccgcgc catagacctg
     2461 gccatacatc atctccgtcg accgagaccg ttcgcgccaa tctttggcga ccaagccacc
     2521 gaacccgccc cacgatgacg tgagcgacca ggtgaagcgc gcctccggaa acgctcggag
     2581 cgcatcgatc actttgagag aatcccgatt cgtggccgcc accgtcgaat ccggcgcggc
     2641 ttccagaaac aggctgatgt gactctgatc ttccaccggc gccagctcct gccgggagaa
     2701 ctgatacagc ggccaggccg cgaccatcac caagagggcc acgaccacaa tcgcccagcg
     2761 catctccaag gccccatcaa gcagacgtcc atagcctgcg cgcgtggcgt cgaagacccg
     2821 attcaccatc ttggtcaacc gaccttcctt accttcagga tgaacaaaat acgagctcat
     2881 caccggcgag agtgtgatgg ccaccacgcc ggacaacacc acggcggcgg ccagcgtcat
     2941 cgcgaattcc aaaaacagcg acccggtcaa accaccttga aagccgatcg gggcatacac
     3001 cgccgccagc gtgatcgtca tcgcgatgat gggcccgacc agttcacgcg aaccgagcag
     3061 cgccgcctgt attcgggatt tcccctcgcg cacatgacgt tcaacgtttt cgacaacgac
     3121 gatcgcatcg tccacgacca atccgaccga caacacaata gcgaggatcg tcaacaggtt
     3181 caggctgaag ccgaatgcgt acatgaagat cgccgcgccg accaacgaca ccggcatggc
     3241 gaccagcggc accaacgctg tccgcaccga acccatgaaa agaaacacca ccgtggccac
     3301 gatcagcacc gtttcaccga gtgtcttggt gatttcctcc aatgcgctcc gcatgaacat
     3361 ggtgccgtcc cacaccaact gcatatcgat atccttcggc agcgtggggc gaatgcggtc
     3421 catttcctcc cgcaaccggt gcgccacctc gatttcgttc gaacccacca ggggccagat
     3481 accgagatac accccttccg tgcggttata cttggcgacc atttcagctt cctcagcgcc
     3541 gttctccact cgcgccacat ctctcaaacg cacgatggct ccgccgcggt ccgtgacgat
     3601 gaggtcttcg aactcttcgg tgctgcgcag atccgtattc gccagcaaat tgatctgcac
     3661 caggttgccc ttcgtccggc cgacagccgc caggtagttg ttgcgccgca acgccgcttg
     3721 cacgtcgccg ggcgacaagt tgtaggcggc gagacggtcc ggatcgatcc agacccgcat
     3781 ggcgatctgc cgtccgccct cgatcgtcac gcgctgcaca ccggtcaggg tggcaaattg
     3841 cggctggagc gatcgcagca accaatcagt caaggccggc accgtgcgtt ccggtgacgt
     3901 gaagctcaga taaaacgagg cataaggacg atcggcccgc tgcacctcca ccgctggtgg
     3961 ttccgcctcc gccggcagct cagagcgaac ctgttgcagg cgtgccgtga cttctgccag
     4021 cgccgccgtg ctgctgtggt tcaacttgag gtgcaccgtg acggtgctga tgcccgcccg
     4081 gctggtggat tccagatagt cgacgccgct gatggccgac acgacccgtt caatcggcgt
     4141 ggtcagaaag ccgcgcaccg tctcggcgct ggccccgtaa tagacggtgg tgatgatgac
     4201 cgacgaactt tcgatcttag gatattgctg caccggaagc gtcgtcagcg cgcgccaacc
     4261 gacgaggacg atgaccagat tgatcaccac ggccaagacc gggtgcttga tgaagacatc
     4321 agtgaatgaa cgcatggtca tgcctctctg atttacggtt gctccggcga aggagttgcg
     4381 tcggacttgg cgaccggccc accggccgcc accaccaaca cgccatcacg caatttaaac
     4441 gcaccggatg ccgccacctg ttctccggcg gccaacccgg catagacaat gatgtcatca
     4501 cccgacatcg caccactctc cacgtgacgc acctgcgcgc gcgatttgcc ttgaccgtca
     4561 gaattgatca caaatacctg atcgcctccc ggccccttgc gcaccgcatt caccggaatc
     4621 gccaccgcgc tgcgcggcgc accgacccgc accttgatcc gcacggccgc gcccggtgtc
     4681 ggcatcgagg gtgaccgatc gattcttgcg cgcaccatgg cattgcgtgt cgcgggatcg
     4741 atgcgggcat ccaacgccac gatcttggcc tgaagcacag acgtgtcacc cgccggatac
     4801 acatccacca ggtcgccgtg tcgcaatccg gccgccacat gctgcggcac ggcaaagtcc
     4861 acatgcacgg cttcatcgat gccttgtagc gtcgtgagct gcgtgccttc attcagatat
     4921 tgtccggggt gcacgtcggc gaggccgacc cgggcacgga acggagcgcg gatggtcttg
     4981 cgcgcaatta acgctttggt gcgtgcgatt tgggcctgca cgacatcgag atccgccccg
     5041 gcgcgatcaa cctcttcctg cgcggcagca agatcctgtg tcagcgcccg gcgtcgatcg
     5101 aggacagtac gggccaacac cgcctgggcc tgctgtgccc gaagatcggc ttcttccacc
     5161 gacacatcga gggcaaccag caccgtgccg gcttcgacaa tctgccccgg cgtgaaccgc
     5221 acctggctga ccgtcccggc taattcattg cggagggtaa tggagcggag ggccaacacg
     5281 gtcccgatcg aggtcgcagt ctggtgatgg tcaacggccc gggccactgc gagcgtgacg
     5341 acttccatcg gttccggctg attggtcgac gcagcggctt gatcctggat gctgccatat
     5401 ttccacaccg ccaacccgac cccgattccg atcaccgtca gcatcaacaa cgacgatccg
     5461 atccatccac gacgattcat gaccattcct cccgaccatt gacagatgaa actggcgaca
     5521 gactcacttg atcaacagaa gcaacgctgg ctgatcacag caaatcgcgt tcctgccgaa
     5581 cccgtctaca ccatggccgt gaaccaccga ttcttctgcc ataattcacg ccacttcttt
     5641 ctcggcagcg ccggatgctg tcgcgtgccg aattccgact gccttcccgt cggcatctgc
     5701 cgaaataacg gcgcgatcat cgaacggcga accaaatcga ggcggaggtg gaatagttcc
     5761 ttcttcacaa catggcggag attcgtcacc tcaggcaggg tgacctggag tgccgaggca
     5821 aaccgctcca cgagttccgc ctctgcacgc ttcacgtcct ggtcgatcaa ggacatcacg
     5881 atcaggccgt tgacgagttg ccggcggatc tgctgatcgg gcaaccctgc cgccaactcc
     5941 tccggcgcaa gtggttggag gccgtccacg tcatgatgtg tgccgaacac gcgctgtacg
     6001 gcttccagca tatgtcgctc tgattcatcg accacaccgt ccgccagcgc cacggttttc
     6061 atggcgcgta atccccaatg ggcctgctcc gtcgtgggca ttgtgaattc catcagatgc
     6121 ctccctgccg acgtgagtgt attgcgcgtc agactgccag tctctccacc tgtatcatcg
     6181 gcaccggttc aggcccgtcc acgatcgccc gccacgcatg aagcccaccg gccagcggcc
     6241 atacccgcgt gaagcctttc cgttgcaaca gcatcactcc ctgtgcgctg gcggcatcag
     6301 ccgggcaagc gcaatagagc accagatcgc gggagcgggg aagttcccca tgccgccgcg
     6361 ccaaatcctc aagggacatc tgcagggcat taggaagacc gggctctgcc tcaacggcaa
     6421 gccgcgtccg cacatcgatg agcaagggag gatccggcgc tgccaacttg tccagcagat
     6481 ccgccaccgt catcctcgga acggcttgaa gttggcggcg aacatgccag gccttccagc
     6541 cgatgtaggc gggcagcagg accactgctg cgagcaggag tgccggtacc acctgttccg
     6601 aataggtcag ggccaacgcc acctcctcag tgaagagcag gccgaacccc aacccggacc
     6661 ccgcccacac tgcagcaccc agcgcatcgt agagcaggaa caccggaagt cccaggccga
     6721 caattccggc taagggcggc gcgatcgtgc tcaatccggg aatgaacttc gcgaccaaca
     6781 gcgattgcac gccatgcttg cggaaaaagt cttccgtccg ccgcacgcag gcgtccggct
     6841 ccagcgcgat ccggcacaac agcgtcagca ccggacggcc ccgccagcgc ccggcaagaa
     6901 accagacgat atccgccagg agggtagccg acagcgcggc gatcaacgcc ccggaccagg
     6961 agaggtgacc ggtccccacc aacacgcccg ccgcgacgag cagcggcaat gccggaaacg
     7021 gcaatccgag ttgctcggcc agcacaccac cgaacaacac ccacgctcca tgctgctcca
     7081 acaactcagt caccgattcc atcagccgct ccttccggtt cttccgatct caatacagtc
     7141 aggctcacag caggatttgt tccaggccga ggagccgcgc ccgagaacga gaattcgttg
     7201 cggcgaaagg cgattccaac gaaggagccg tgagacggtg atcaaaacag gtgccggtgc
     7261 cgaggagtga ctgccgacat ctcggcagat gtcgactgat cggcacagac agaagaaagg
     7321 ggacgagtcg gaggttcggt tattggggac ggacgatgcc gagcttctgc atcttggact
     7381 ggagcgtcgt gcgcttcatg cccaagcggg cagccgcgcc ggacgatccc ccgatgaccc
     7441 agtgggtgtc ccgcagggct ttgatgatgt ggtcgcgctc cgcatcctcc agcgtcgtgg
     7501 ccgacccgtt ggacggagac accggacgtt ttaattccgc agtcggcacg aacagctccg
     7561 acccggatga caggatgacc gcccgttcga cgaagttttc cagttcacgc acgttgcctg
     7621 gccatccata ggcttgcagc gcccgcatgg catcggtcgg caccgtttcg atgtgctttt
     7681 tcatgcgcga ggcaaacttc tggacgaagt gccgcaccag cactggaata tcttcgactc
     7741 gttcgcgaag cggcggtacg gtgaccggga agactttcaa gcggtaatac aaatcgctgc
     7801 gaaactgttg tgcgtccacc atgtgactca ggtcgcgatt cgtcgcggcg atcacccgga
     7861 cattgacccg catcgtcctg gtgctaccca gccgctcgaa ttcctgctcc tgcagcacgc
     7921 gcagcagctt gacctgcaat tcgagcggga tctcccccac ttcatcgaga aaaatcgtcc
     7981 cccggtccgc caattcgaat cgcccgatct tcgtggcgat ggctccggtg aaggcgccct
     8041 tttcgtgccc gaagagttcg ctttccaaca ggccggttgg gatggcggcg caattcagct
     8101 tcacaaaggt ccgttctcgg cgagcgctca gattgtggag ggcgcgggca atcaattcct
     8161 ttccgctccc cgtctcaccg agaatcaaca ccgtggaatc agtggccgcc acggtctgca
     8221 cctggttcaa cacccgcttc agcgcgcggc tttccccgac gatttcctcg aaattgtaat
     8281 ccgtttggat ttcttcttcg agatagactt tttccttggt caacttgtct ttcagtgcgg
     8341 cgatctcacg gaaagccagg gcattttcaa ccgcgatggc aatttgttgc gcgacctggc
     8401 caagcaactg cacgtcctcc ggatcgaacc ccgcgtcccg ccgccgtcca acattcaacg
     8461 cgccgatcga acgattgcgc gagagaagcg gcacggagca aaaggacttg atgccgcgat
     8521 ccagcaaatc ctgcgcacag ggtgaggcgc ctcccatggc cagcagatcc cgctcattga
     8581 acaaggccgc tttaccggtc gtaatcgcca ggcaggaggg cgactgcgtg gtctcctcga
     8641 tacgcccctc ttcaatgaaa ttttcgtttc gatcgaaatc cagcacatgg atgcgccacc
     8701 ggccggtttc ttcatcgcac agcaacaggc tggacccgtc atgctgaatc acccggcgca
     8761 ggcaggcgct gaccgccggg aagagctgat ccaaatccag atgcgagacg acggcattgt
     8821 tcacctcgag caacagccgt tgccgatcgc gttccctggt caactgcgac tgataggcca
     8881 gcgtgctctc cgagttgagc gcattgtcca acgccaccgc cacttgctgc gcgacctgtt
     8941 tcatgaaggc gatctcgctg tcgagataga cccggggccg caaacttccg aatcccatcg
     9001 cgcccagacg ccggtgcgca gtggtgagcg gcaccacaca aaacgaacgc acgccgttcg
     9061 cgcgaaacag ggtcatcaag gcgggaaacc gtggctcccg ctcgacgtcg tccaccgtca
     9121 acggttcttg tgtcttccac accaggccgc ccggtgattc gtcgacgggc gtttccaatc
     9181 ccggttcgat ggtggactgc tcagacgtga ccaggagcca cagccgcatc acgtcacgcg
     9241 aagggtcgtg gcggacgaca ttcatgaaat cgaatggcac cacctggggc aaccgcttag
     9301 ccaggtcggc caacaacaga ctctgatcat gatgcgacgc gatcgactcg gtcacctcga
     9361 gcagcaggcg caatcggtcc cggtcgcggg cgagatcctg gtgatgcagc acattgtcca
     9421 cggcgaccgc caccagtttg gcgacctgat tcagaaagga gacagtccct tcgtcgaaag
     9481 cgaagggctt caggcagccg aaaccgatgg cgcccagccg gcgcatcgcg gtagtgagcg
     9541 ggaagaagca gactgattgg acgccgattc cccgtaaggc gtccatcccg acttgaaacc
     9601 gcgactcggc cgccagcgac gggatcagaa tcggttgttg atgctcccac acccatccgc
     9661 tggccgagtc cttcatcgga atctccagcc cgtcgagacg gcgcgtcacc cgtttcgctt
     9721 ccgccgtttc cagcacatgc acctgcatca cgtgcttggc ggcatcgtgc aggaccagcc
     9781 cgacgaaatc gaatggcgcc accacaggca ggcattgcgc caggtcctgg aagagcgacg
     9841 gcaaatcgtg gtgcgcagcg atgacctctg ccacctgcag caacgccgca ccacgatcgg
     9901 ccatcatcgg ttcaacactg ctcggcatca tgtcatgcat gggcatagta tacggtgcca
     9961 tccggcctcc gatgcaactg ccgcgaggcg gccccgcccg ttcgtgcgtg aaccatcagc
    10021 gcacatcaaa ttcccaggtc atcccctgcg cgaaatgcgg cgcgccggtt ttcttatccg
    10081 ggaagaagca gatcaaggca tagggccctg gatcgaacat ggccgtgaac agaccccggc
    10141 tcccctgttc aattcctaca atgcccccca acggccgccc cggcggaggg ccgacagcgc
    10201 caggttcgaa cgccgcgatg aagtccttcg ccgtggcagc cggcgcgagc ttcaccagca
    10261 cgacctcgtg agtctggctg ccttcgttga gcagttgaat ggtgtgcgac ccggcggtga
    10321 tgggcttgga caacacaaac tgaaaatccc tggcggtaat gaccacatcg ggcttcggct
    10381 cagcgatgcg ggtcccgatg gtctccgtca ccatgaacgg cctggccatg cccatcgcca
    10441 catgcgccac gccggccttg ttcgggacaa gacagagcgc cagatgttgc cccggttgca
    10501 ggtccatcac ggcagtcgcg cgatcccccg gaaccacggc attcgggcca ccgacaaaag
    10561 tggcccagat cggcggatgc tccggatcgg ccttcattgc tgcggcgaag tcggccggcg
    10621 tcttgcccgg agccaaccgg acaagttgcg catggtggac ttcagcgcct tcgttgatca
    10681 cctcgatggc ggtcaggcct gacggcacct gctccgggct tttgaagcca tagtcggtcg
    10741 cggtgatcgt gacgttcccc ggctgcgctg cctgcgcctg tccggtgaga ggcgtggcgg
    10801 ccagcacggc cagacacagt gaaagaagta tatggctctt catgatcatc ctccttgatc
    10861 gtggactgcg ttccaacacc agcactgtcg ggagggaacg tatcacggga ggcgccgact
    10921 tccttcctaa gaatatgcta tggtgtccct aaatttttcc taatgtgatg ccactacgcc
    10981 tgcaatgatt cctcctgcca tcgtcgatct gctgcagacc ggcgtctccg tcaacgtcgg
    11041 cacacgtgat aagaatttca tgccggaatg caccagaggc tggggcatct gggtagcagc
    11101 caatcgtacg accgtgacgc tattactcac ggaaccagca tcggcccgta cgctggccaa
    11161 cctccgtgac aacgggctca tggcgattac ctgctcacgc cccacggacc acgtcacctg
    11221 ccagctcaag ggtagcgtca cgcagatccg tcaggcgact ccacaggatt acgagcgaca
    11281 acagcagtgg cgccatgcgt ttctgggcga actcattgcg gtcggcgtgc cggccgagca
    11341 gaccgacgcc ctcatcatgc agcccgccgt tgcaatcgag atgcaggtca ccggcgtctt
    11401 cgcccaaact cccggccctg gcgccggcga acaggtgtga ggcatgggca ctgccgcgct
    11461 ggcactcaaa gatctctggc cctgcttcga aggcatcatc ccggccacca tcgcgacctg
    11521 cgccaaagac ggcacgccta acatcagcta tgtcagccag gtgcattacg tcgatcccga
    11581 gcacgtcgcg atttcgtttc agttcttcaa caagacgcgc aagaacatcg ccgagcatcc
    11641 cttcgcctcc gtgatggtcc tcgaccctcg cgcgatgcag gcctatcggc tttcgctcac
    11701 ttatgagcga tcggaatcct cgggaccttt gtttcaagcc atgtctctgc aactgcaggc
    11761 catcgcctcc catgccggca tgtcggacgt cttccgcctg cgcgccgccg acatttacgc
    11821 cgtgcgtcat atcgagcggg tggacggctt cacctcgtgg cctgtcccgg cgcacgacga
    11881 gcgaccgccc tccctgttca tcgatccgaa cctgcatgcc ttgagagtgg tcgcggaaca
    11941 gttgaaccgg gcggaatgcc tcggcgaact gttggagacg gcactccgct gcctggatga
    12001 gttcttcggc ctgcaccaca cgatgctgct actcgtggat gagaaacgcc gcacactcca
    12061 gaccattgca agccgcggct atgacgagaa cggggtcggc tccgaggtcg gtttcggcga
    12121 aggcctcatc ggcatggtgg cggcggcgaa actgccgctg cggctttccg gcctcgacta
    12181 cacccttcgt tatgcccgtg cggtacgtga tcgtgcccaa gcgctctccg gcccagaatc
    12241 cctgtgccgc gaaattcctc tccccggcct ctccaatgcg tccacacaga tcgcggtccc
    12301 gctgatcatc agacaccgct gcctcggcgt gttggtcgct gaatccttgg agccgctcac
    12361 gctcctgatt cgcgaagagg cggtgatggc cattgtggcc ggccatctgg ccgccgggat
    12421 cgagcagtcg gcacaggaag cggacgactc cgtggcagtc gaggcccccg ccgcagcggc
    12481 accgccgctc cactcaccgc tctcgcaccg ctcgttctgc ttctaccagg ctgatgactg
    12541 catcttcgtc gatggggaat acctcatcaa gaacgtgccg ggacgcattc tctggcggat
    12601 cctgaaacaa catcaggatg aacaccgagt ggagttcacg aatcgcgagc tgcggatgga
    12661 tagctggctg ggcctgcctg aatggaaaga taacttcgaa accagattga ttctgttgcg
    12721 gaaacggctg gagcagaaat gccccgatgt gcgacttgtc ccgcgtgggc gcgggcgatt
    12781 tgccctcgaa acttccgtca agattatcct gtcggaaaag cccgcgtgag gacggcctac
    12841 gagcctcctg acacctgagt tgtcatcgat ccaggtcctc ttccaacacc gccggtggta
    12901 acgccgattc accggttcgt ccggctcgac ttctggcggt aaactgcgcc cccttagccg
    12961 gccgaatcgg tgcgaaccgc aggaacccga tgtcctgcag gatgtcgttg tagaccgcct
    13021 ccatcccgcc gcgcatgaaa taggcctcat gccagaatcc tgttccgccg gtatcacgca
    13081 gaaactgctg ccaccactgc cgatggggat cggaacgagc ccagcgttcc agcgcatcga
    13141 agtcgcgcca atattgccgc attccaacgt gcggcggcat cagcgaccag agcagtgatt
    13201 catgaagcaa cagtccctcc ggctgagcgt cgactgatgc ggcgatttga ggaccgaacc
    13261 cgaataaggt cttcagccct gtccagcggt tgacccgcat gccgagatag atgacgacga
    13321 ggtctggaaa gccggagagg tcgacggttc tgcgtaggat ggggacaggc atcgtttcct
    13381 tagcctagat ggcagaatta gtaatcgacc atgtgatggg cgactacttg ccgcgccgca
    13441 caatcaccgc cgccacctga tccagccgga tggtcgcatc gaagaactcc atcccggata
    13501 gttcggtcag ggctaccgcc tgcgtgccga ctttggccac cttgccgtcc acatcctgac
    13561 ccgagagcaa cctgaccttc acccgtttgc cgacctgctg ttccagaatc tgacgcaaca
    13621 cgtccggact attgaactca atcgtcggct cctcagccct ggtcaacgga accgcgagag
    13681 tccctccagc caagacacac cacagcagga ccaccaggcc caagccgatt cgtccgttac
    13741 gatgcaccat acgaaccctc ctcaaatctt tgtgagtgaa ctgcgcaaga gtacgtgata
    13801 gtcgttcgag tgccatccgg tgccgtggag gatacccgga gaggcgtgga aagtcacgca
    13861 accgtcacgg gattgcctgg caccggatct gagcctatcc aagccgcgat gcccccctgc
    13921 aggaaggccg cgcgatcctt tacagattgc aggggtcacg gtatcatccc tgaagttcca
    13981 tacgtacctt cacccaaatc cgcaaggaga cgccgatggg ttggatcatc ctcgccgtgc
    14041 tggttgcgct cgtgctggtc gtcatcggga tgtacaactc gctggtccgc ctccgagccg
    14101 cggccgaaag cgcctgggct gatatcgatg tccagctcaa gcgccgctac gacctcatcc
    14161 ccaaccttgt cgaaaccgtg aaggcttacg cggcgcacga aaagggcgcg ctcgaagccg
    14221 tgatcactgc ccgggcgaag gccatgtcgg cccaaggccc ggaagccaag gccgtcgccg
    14281 agaatcagtt gagccaatcg ctgaagtcgc tgtttgccct ggctgaagcg tacccgcaac
    14341 tgcgcgccgt cgaggctttc aatcaactcc agggcagtct gaaccaggtg gaagacgcgc
    14401 tgcagaatgc caggcgctat tacaacgctg tcgtccgcga ttacaatgcg aagaggcagg
    14461 aggttccgac caatttcatt gccggctggt tcaactttgc cggccgccag tttttcgagt
    14521 taaccgaaac cacccagcgt gagccgccac gggtgcagtt ctagcgtgga tcgtgccggc
    14581 gatccgatcg tgaaaccccg acgacgtcca catttcgtcc acgttctctc cagcctgatc
    14641 ttcctgtgcc tcattgaatc cgtgagcctc ctgccggctg atgcgcgttc ctttgtgctg
    14701 agtcgtttcg atgtggagct gcaagtgttg tccggcggcg agttgctcgt gacggaaacc
    14761 gtcagcccgc gcttcgaagg cgcctggaac ggtatcgagc ggctgattcc cgtggaatat
    14821 cgtactccgc aagggttcaa ctacactctg tttctggatc gcatctccgt gacggacgag
    14881 cagggcaccc cgctcaaatt cgaaagcagc cgggagcggc attaccggaa cctccggatc
    14941 tggattcccg gcgccgcaga tgccacccgc accttcgtgc tgaaatacct tgtccggaac
    15001 ggcctgaaat ttttcgagga ccatgacgag ctgtattgga acgtcaccgg cgacgagtgg
    15061 gatgtcccca tcgagcaggc ttccgtacgc atcctccttc ctccgcaggc gaccggcgtc
    15121 cgtgcccagg ccttcaccgg agcctatggc gcgcgcgaac aggcagcgac cgtgacgatc
    15181 ctcggttcgg ggatcgagat gcggatgaca cgccccctcg gctttcggga aggactcacc
    15241 gccgtcgtcg ggtgggacaa gggcctcgtg gccgccccca ccgtggtgga gcagacccgc
    15301 atgacgctcg tcagcaattg gcccttgggc atccctctac tcgcgttcct cgtgatgtat
    15361 cgcctctggt ccaccagggg aaaagatccg cgactccggc cgattgccgt catgtacgaa
    15421 ccaccggacc agctgacacc ggcagaagcc gggacactgg tggatgaaag tccggacacc
    15481 cgcgatatca ccgccaccgt agtggacctg gccgtgcgcg gctatgtgcg aatcagcgag
    15541 cgcaaggccg aacatctgtt cgggctctgg tcgagcatgg attatgcgtt tcagcggaca
    15601 aagccacggc aggaatgggc ggccttaccg tccttcgagc ggctcttgct ggaggctctg
    15661 ttcaaggata gcgaagtcga tgagattctc ctcagctcgc tcgaaaaccg cttctacaaa
    15721 tctctcccag gaatacacga cgcgatattc cagtccctgc aaaaacgcac gtattacagc
    15781 cagcgccccg accgggtgaa acagggatat ctcatcggcg gcattgcctt cggaatgctg
    15841 ctgaccatcg ggcttggcat cctcgctgac cggtggggcc tcgcgccact cacagtcttc
    15901 gtggccggct tgctttcagg gatgattgtc atcggattcg gccgcatcat gccggctcgc
    15961 acacagcaag gcacacgggc gttggaaggg gtgctggggt ttgaggaatt tctcacgcgc
    16021 gtcgaagccg accggtttga tcgcgtcatc aagacgcccg aactgttcga gaaatttctg
    16081 ccctatgcca tggcgctcgg agtcgaaaag aattgggcgg cagcattcga aacgatcgtc
    16141 atgaccgctc cggcctggta ccaaggcagc gatctcacgc agttcaatac gagaggattc
    16201 acgagccgca tgggtgacct ggcgtcacga accggctcga cgatgacatc cgctccccga
    16261 agctccggcg gatcaggatt cagcagtggg ggatcatcgg gcggcggatt cggcggcgga
    16321 ggcggccgcg ggttttaacg cgcggccgca ctgtcactgc acctgcatgt cacctggtcg
    16381 ccttgtgctg cccgctcgtg ttgggtgccg gaaccccgta aaattcatag atgacggtgg
    16441 tctgcgccag atcgccgaaa tccggccaat ggttcttatc gaaccccggc gcgtttttga
    16501 gatcccgttt tgtggcgtcg atgagaatct tgtctttacc cgatgccacc tgcagcgcat
    16561 cccagggaat ggcgaaatac ttgtctttga tgcccaggaa cccgccgaag tccaggaccg
    16621 cataatcaat tgctccgtca tcgggattga tcaccagatc cttgatgtcg cccagattct
    16681 ttccgtccgt tccctgcacc ttcatcccga tcagttcact ggccttgaac actccgttgc
    16741 gatcccgagc gtcggcatac tggacggttg tggcaacgga caggcacatg accacgatgg
    16801 caatgacgta ccgcataggg ccctcctttc gtcgcctatg caaggcgacc tctgcgccat
    16861 ttaacaacga cgccaggagg aacgtgcctc tcaaaaaccc tagttcgaca cgcacgggta
    16921 agccggcatg gtctcgcctc aaaggagcgc gaacggtttt gaggccggca tgccctctcc
    16981 atcgaaaggt cacaactcat agattcgatc gccaccgatc gcgaaccgcc gctcgtcagg
    17041 acgcagaggt acgtcatacc cgccgctgac ggatgaaaag gccggcagca caccttgtcg
    17101 gtcattgatc acaaaggcgg ggagcctcac ccggtccggg ccttgcgcca attgaatcat
    17161 cggatggaca tgtcccaccc aataaaaggt gtctgcctcg acggttccct caattggctg
    17221 gtgcttgaaa cgaaatggag cgatggtcac ctggtcacac cgctctgcct tcacccattc
    17281 cttcggccag cggcgagcga gtccgccatc atggtttccc agcacgaccc ggaccgaccc
    17341 gctgaactcg tgcaaccatc gcgcgaactc tgccacgacc gcgtcagtca ccccgctcaa
    17401 cgaatggacc aggtcgccca agaacaggac gtgctgtgcg tcaacactga gcacgagcgc
    17461 agagaggcgt gtaaggtcct gttgtcccga ctgcgagggc aaccacaatc cctgctgttg
    17521 aaaagtctcc gccttcccga gatggcaatc cgccacgacc agcagcgaat gggtcggcca
    17581 aaacatcgcc cgttcgggaa gcagggcgac acattcgtct cggatacgga ttctcattca
    17641 gcgaaacacc tcccgttgca accgcgcgac ccgctgttcg agcttttcgg tcgagaggcg
    17701 cgacttgatc cgctccacat agagaggaaa ggcaaacggt gtcagccgcg ggatgggaac
    17761 gatcaacagc tcctgctcgc ccatccgacg catcaggccg cgcagccggt cgatctgcaa
    17821 ctggcgatcg tggacttcac gaaacgcttg cgccagcagc ggatgagacg gttcatactg
    17881 tcgaaacaca tcgaacagca gcgaggcgct catttgcaga tgacgtgccg ttttcctcgc
    17941 ggacggagtg ccctggtaga tcagccccgc aacccgcgcg atttcgcgga acgcccgcct
    18001 cgcgagctcc ggaaagttct gcgacgcgtg gatgtcctcc tccaacccgt cgagagtggt
    18061 cagcgcctga ccgatctcac ggtcactgcc gagcggttcc gtggagagca actcgaagcc
    18121 gtagttggtc gccgagacag caatcgtgtt cggacgcagc cgcgacagac gataggccac
    18181 cagatgcccc agcccttcgt tgacgctcca gccttcccag gtgtacacga acacatgatg
    18241 cccttcggca gtccggcagg tttcgatcag cgtctgcgcc gtggatggaa tacgtgaccg
    18301 gctggattgc agacgcgcga tcggcagcaa cgcatcaatc tcgggcgacc gtgaattgaa
    18361 tccttccacc gcctgcctca ggcgatcaac ttctcgccgg aggtagaaag acagttgcac
    18421 gctgagcgga agaatggtgc cgctccagac cgcagcgacc ggactccgtt gacgggccgg
    18481 cttcacatag gcggtcatat cccgcatctg cagaaactgt acggtgcggc cgccgaactg
    18541 aatgacatcg ccgggtcgaa gcttcgccac gaacgtctca tcgatcatgc cgagtctggc
    18601 tcctcctcgg acctgtaccg tcacgccgct gtcggagagg attgtgccga tgttcatctt
    18661 gtgccgccgg gccgcctccg tcgaaacaaa ccgatagcgc ccctcgacca cttcgacctt
    18721 gcggaattct ggataggaag acagggctcc tccggaacgg atgaatgcca acgcccaaga
    18781 ccattcgtca tccgtgacgg ttcgaaagga gacagccgat cggacttgcc ggcagagatc
    18841 ggctggatcg aatccctcgt tgaatgaggc attcaggaga cactgcacca gcacgtcgag
    18901 cggcttgtcc aacggcgtcc gttcctccaa gacgccaccc agcatggctt gccggagggc
    18961 cgacgcctcc aaaatctcca tgacattggt cggacagagc tgcaccagcg tgtcctctcc
    19021 cggccgatga aacgcgcgac cggctcgctg aaccgcccgg gcgatcgatt tggcagatcc
    19081 aatctggatg acattttcaa ccggcgggaa atcgaccccg aggtcgagtg aagaggtcgc
    19141 gacgaccaat cgtaaggcgc cgcttttgat gccatcttca acgcgctgcc gttcgtcctg
    19201 atccagcgat ccatggtgca aggccatgac ggtcgaccat tccggcctgg cttcgaggat
    19261 cgcctgatac caccgttcgg catgcgacct ggtgttagta aacagcagcg tgctttgatc
    19321 gggattgatc tgcctgacga cttccggcaa cagactcaat cccgaataac caaaccaggg
    19381 gtaacgttga atggactccg gcaggaggca gtgaatccgg atgtgtcgtg gaatgtccgc
    19441 acggatgatg gtcggctcaa ggccgctccc gaccgcgatc gacgccgctt cctccaagtt
    19501 cgcgatagtt gccgacaggg cccacacccg ggctccggcg gccatcgcac gcaaccgggc
    19561 gagcgtcaac tccagcaaac tgcctcgctt cgttcccagc agttcgtgcc actcatcgag
    19621 gatgatcgcc tgaacagctc gcatccgatc cttccaccct tgatcggtaa gtaagatcgc
    19681 aagtgattcg ggggtcgtca cgaggacatg gggcaatcgt tctttgaatt tccggcgcgc
    19741 cgtcgcggtc gtgtcgccgg tgcggagatc cacatcgatc ggccagccca gatcgtccaa
    19801 cggctgttga atggcctgcg caatgtcccg agccagggcc ttgaggggcg tgatatagag
    19861 aatcgtgagt ggcggctggt ggccagctat cggacgggcg atcaggctca ggagcggccc
    19921 tccaacggcg gcgtaggtct tcccgctgcc cgtcggcacc gcgaccaggc ctgagcgtcc
    19981 cgcgtcaaat gcctgccagg cctcaacctg aaatggaaac ggcgaccatc cttgccgatc
    20041 gaaccactcg ttccattgtt tccacgcctc ttcgcgtgag agcgtcggcc aggcttttcg
    20101 acgagcacgg gcttgttcct cagcatcggg ccgtggacgc agcggcccca tccggttgag
    20161 ccgctggatg cctcgcagga ctttcgccga gctactcatg aaccgacatc tccagccccg
    20221 agttcgaact gcagctgctc ctgccccggc agggactccg cccttgctct ggcgcgtccc
    20281 ggccggccag ccgacgaacc gaaggatgcg agcagcgttt caagtgtcgc gacgtggtcg
    20341 gcatcctcca cgcgcttgtc cgttcgttcg cgcagaatcc ggggaaaccg gagcgcgaac
    20401 ccggatttgt gccggtcgct ctggctgata ccctcgaaac cgatttcgaa cacgcggcgt
    20461 gcctcgacca tgcgaacagg gccgaaccgc tcgcgcgtat gcttgcgaat ccagccgtcg
    20521 agttcgcgaa tctcttcgtc cgtgagacct gaataggcct tcgcgactgt gatcagacta
    20581 tcgcccgccc agacagcaaa cgtgtaatcg gtatacagcg acgcgcggcg gccggttccc
    20641 ggctgggcgg cggtcagaac ggcatcgatc gccagcggat cgattttcca cttgaaccac
    20701 acgccgcgct tgcggccggt cacatactcg ctgtccttcg ccttcagcat gagtccctcg
    20761 gcgccctcta cacgcgacgt ctcacgtaac agtctggcct gctcccacga tgacagcgcc
    20821 agcagcggac tgatccccag acgctccccg atgtacggat gcagtgtgcg ttcaagcatc
    20881 gcctgccgtt ctgcaagggg atggtgccgc catgaccgtc catcggcttg aagcaggtca
    20941 taggccaaga atgaaacagg actggaggcg atgagcgcca gcgagggttt tttgcggttc
    21001 aaccgccgct gcagagattg aaacgcatcg ggacgatgcc aatcccctgc cacaatctcg
    21061 ccgtcgataa cccagtcgcc ggataccggc gagaggagtc gcgacaggtc gggaaaggag
    21121 ccggtgatcc gctcctcgcc gcgtgaccag atctccaggc gcgagccgga tttcacgacc
    21181 tgacagcgga taccgtccca cttccactca cattgccagt tcgatatatc tcccagttcc
    21241 tccggatcac cctccatcgg agcagcaaga caaaagggaa gtggtaacag ggtctcgtcg
    21301 gacctctgcc cggtctgagt ctcttccgcc ggcgcactaa gacggagaaa gaactcggcg
    21361 ctgggttgcc aggttcccac aagccgggaa gcaatcaccg caggactcag ggaaaacgcc
    21421 gcggccacgg cgcgatacac gagcgtttcg gacaccccga cccgtaacga tccggtcagc
    21481 aacttgttca agatgaacgt ctcattggtc tcgagctctt cccaccacgc gagaatgttc
    21541 gagcggcgag cctgatcgtc catccccgcg agcggcaaaa tgcgctggac catccactcc
    21601 cccaacgaga gtcgagacaa cggcgcgtgc ttgacggctc cttctcccgc agccagcaca
    21661 agggcgatca gctccgccgt atcgcccaca gctgcatagg attcctccat caaccaatcg
    21721 ggataaccga ccttctcctg cgcccaacgc cgcagcttgg tggacccgac gatctgtttc
    21781 ggcctttgac ccgagaggaa gaacaggacc caggcgctgg tgtcgggatc ctggtgacgg
    21841 aagaactccg tcatgatccg caccttttcg ttcgtcgcgg tcgtctgatc gagttgctcg
    21901 aacagaatgg caaacgcctg cactagtctt ctccctcgcc gaagtgggtc gtcagggctt
    21961 cggccttcgc tcccttgacg tctcggaggt atcgagccag cgtgtcggag gtgccgtgcg
    22021 tcaccaacac acgagtcgcg cgcgattctt cgaccgtgtc gagaagactt ttccagtctg
    22081 cgtggtccga caggacgaac ccgcggtgat agccacgccg gcgacggatc cctcgaaccg
    22141 ccatccaccc ggacgcaaat gccgtggaca cggcacgaaa ccgcttcatc cacgggctgc
    22201 gatgggcgga aggcggcgcc aggatcagcc gcccttcggt aggaccttca gcatcgctca
    22261 ccagcctggt cggaaccatc gagacgcctt gttcgcggta aatctgcgtg agtgcggcca
    22321 cggccccatg cagcaggacc tcctcctgcg tgaagtgcgt cagctcggcg aggatgcgtt
    22381 gcgcctttcc caacgcatag caaaacagca gggccgtgac tccctcctcc cggcatcgct
    22441 gccaccagtc gacgatccgc cccgtcacag aacgggtcga ggcccagcga tagatcggca
    22501 atccaaacga cgcttccgta atcatcgtgt cgcagggcac cggttcaaag agttcgcagg
    22561 tgggatcggc gtcacgctta taatcccccg tgacgaccca gacgcgagac ccctgctcca
    22621 cccgtatctg ggcggagccg aggatgtgac cggccgggtg aaatgaaatc cagcaatcat
    22681 cgagccgcac cggcttgcgg tagggcacgg ctcgaatacg gaggtccggg ccgagacgat
    22741 gccggagaat cggcgcgcag ggctccgcgc accaatactc ggcactcccc gtcctggcat
    22801 ggtcgccatg ggcatgggtg atcaaattga cggcagcctg cccccaggcg tcgatatgga
    22861 aacggttgcc cggtcccacg agcccttccg gctgcaaacg aatgaggtcg ctcatcggca
    22921 agtccccgtc cagctgctca ttcgatcatc ataccaaccg cttcattaga caatgaccgg
    22981 gcatgccaga gggattccca aaaccctagc ggtggcgaat tgatctgtac ggggaggaat
    23041 accggcagcg tctgcagtga tgtcgaaggg gggtacgctt atctgattga aacactctgc
    23101 cggatcaggt gctgccgatc accttagaga cagtaggggt cagacccaaa ccggcaccgg
    23161 tggatgatca accggtgccg gtcgtgaggt gagtcgcgac tcatggaagg cctattacag
    23221 agtcgacacc aggacctgct cgatcgaccc ggctaccggg tggagttccg tccgggaaaa
    23281 tcccgccgcc gcaaacatct gctcatactc ccggaacgta taggcgtcgc cttccggcgt
    23341 cgtcgccagc atgatcaggc tgaactccgc cacgggcggc ggcgtgacgc gattctcatc
    23401 cggcacgaac tccagcgtaa tgacgcgccc gcccggcgca agcgagcgat ggatcttacg
    23461 cagcagcgat tcgcatgtct gcacggggaa atggtggaga aagttggtga gcaggacgag
    23521 gtcgtagcat tcgccgaatt cctgctggaa ggcgtcgccg gcgatcgtcc gatagcgcga
    23581 tgcgataccg gcctgttggg cgagttccct ggccacggtc agcacgttgg gccaatccaa
    23641 cgccacgatt tctgcctgcg ggcatcggct cgcaatttcg acgccgaaga gaccgtgccc
    23701 ggccgcgata tccaacacct ttttcacgtt gggctgcgac tgccgaacga agatgccgat
    23761 ctcctcggcc ggcttggcca tcatggggat catggaacgc gcaaagtcca cccagagcgg
    23821 atgctccggg gcgaccgttc ccgcctcgcc ggccaccgtg ccacccttcc gcaccgcctc
    23881 ggtcaaatgc tcaaatgcgg cgatgaactt gggcgcatgc aggaacccca agacagagcc
    23941 catataggcg ggcgagcgac ggtccaaaaa gaccgcactg tccggcgtca acttataccg
    24001 atcgccctct ttggtgagga atccttgtat ggtgaggtaa tccgcgagaa tccgcacgcc
    24061 gcgctcggca gcaccgcaac gctgagccag tgccggcacc tgatcatggc ccagtccaat
    24121 ggcggtgaag agatcgagtt caaccgcagc cttcagcgct tgcgaccgtt gataggcatt
    24181 ggccgtctga aagaataact gcggggtcgg ccccccggcc ggttccggtt tcaacatcgg
    24241 caacactcct cggttcggtg gtggtagcca tgtgagcgct cggcattgac cggattgtac
    24301 cccgtcggga ggatggatgc gagggtgggc gatagcagaa cgacggcacc agcgctggtg
    24361 gcgcacaacc gcgtccgtgg aagaaatact cacgcttcag tgaatagccg gtttatgcgt
    24421 gacgatcaac gaggtaccgg ggggaaacgc agttcccaaa cgaaccgctc gctccgggcc
    24481 gcgcaccgtg acgcgcatgg catgcccgtc ccattgccag gtgatcgaac caccggggag
    24541 cgccaggttg gagaccgcca tcggctgcga caaccagccc gcggtgatac cggcgccgat
    24601 gacgactgtg ggctccgcag cctgctcatc gagatacgcc agcatgtctt gttgcaagag
    24661 caggagcaac gccgccgttt cgtagtcggg cgtgaccgcc aactcgttat gccatccgcg
    24721 cgcatattgc catccgtcgg ccacctcgtc cttcgacgac aaggccgcct cccaggtata
    24781 caatcccggg gacgcctgtt cgccccacag ccgatgcaaa accgtccaga ccgcctctgg
    24841 cttcccttgc cgcaatgcct gatgagcctg cgacaattgt ccggcactcg tggtgtggtc
    24901 cgcagtgggg cgatacgtcg tcagcaattg cttgagttga tggcgacggg acgaaggatc
    24961 agccaaggcc cgcagactca ccggtgacgc gctgccctgc atgctgtcgt gggtagattg
    25021 tcgctgccag ctctccagta acccctgcgc ctgcgtgcgc cagcgcgccg cctgctggcg
    25081 cttaccgagc cgctcggcga actcggccgc ggcaaccagc ccgcgataac tgacggcatt
    25141 cacgtagagc gacggccact cctcacccac ccggccgacg atgagccctt ctcgtgccgg
    25201 ttgcgccaac accgccgttc tggtttggcg cagatgtttg aaatcgtgcg gagccggaat
    25261 cgtatacggc tcgaccagcg gcgttttcgc cgtcagcatc tcttcgatgc gccgggcctt
    25321 cctcagaata tgcggccaca accactcgtc atgcaccttg tcgccgatat agtcggcgga
    25381 ttcagtgagg gcccagatcg tcagcccagg cgcatcggcc tccggaccgg agcctcctgc
    25441 aaaatcatgc atcgcgagaa actgtgagag cacccggctg acgcgaggct cccccgcgcg
    25501 ggcgagtgcc gccgtaatgt acgcgccttg gcgatgccag gcccgataga acacagcagg
    25561 atcgccgggg cgcgtttcat catcgaccag gctcatcatc aagtgcgtga tctgggcgtt
    25621 catcgaagcc tgcaggcgcg gatccggcag cgcgagccgg gcccgcttcg gcgcctgttt
    25681 gtagtagcct tcgatttcaa cgggagggcg caagtctgac agcacgaccc ggatctcttc
    25741 gcccggcttc gtcgttccca gcgagaattg cagccgcgcg tatccccacc cggcctcgcc
    25801 gctccaggac gtgacggccg actgttcggt catccaacca gctgtatttt cgtctcccat
    25861 cgcaatgcgg tcaggagcgg gcgtcgcggt gacggcccac cgatggttca ccgtcacctt
    25921 cccttcctcc tgattcaacg cagtcaccgg ccccccggcc ggccccacgc tccgaagcac
    25981 gatcaccggc acatggttcg tctcattctt gaatcggagt tcccagcggg acgcatcgag
    26041 ccgcgtccac gtcgcctgat aatacggcgt tttggtggtg atggcgggaa tcgtcggatg
    26101 agcggatggc gcgaattctt gtgtgatctc gtggatcggt accgtctgac tgacaaccac
    26161 cggcgcaccg gtcgcatcgc acaaccagag ggagacgccg aaactgggca gttgcgggct
    26221 gaaatctcct cctggctcat ggtaggccgt tcctccctcg ggcgaaccgg gaacggccag
    26281 ggggacatgt ccgaccgacc tgggccaggg ccgctccgga tctttctcca tcatggctgt
    26341 catcagctca cgctggggcg tgaactgcgt cgccgtatgg gtggcggctt gaaactgttg
    26401 ccaattattc agcgcgacac agaccacgag cacagcggcc actcccgtaa cgatgcgtcg
    26461 accgggagcg agagcaccac atgccgcgac cgccaaaagg agcggcatga aattcaaact
    26521 gtagacaaac gtttcctccc cgtagagcat gtgcagcagg acctcgaatg ctaacatgct
    26581 ccccagcacg agccggaacc gcaggtgatg atccagcgtc accaacctcc ataacccatt
    26641 gaggagtaac cccgaccaca ggatgacggc cacgagcccg agcgggccgc ccgagccggg
    26701 agtggaaaat tgaaaggtca gacgctccga gaggcggaag gcatcggcgt tggcctgcgc
    26761 ataggcatca tccttcagaa aacgaacggt tggtgcaacg aggctgtgaa atacaatcga
    26821 ggaggccaca ttggtgatgc ctcctgcttg gggatggttc atgaaggagg cttcctcccg
    26881 ctggccgata aaaaacgccg ccgacggaaa gatgaccttc tgcacgcccc acagaatcac
    26941 caccaggcac agcgcattga tggaaagctg caccgcctgc ttccagggcc atcgcgccag
    27001 gctgacgagt agtcctgcca tccagtttgt gacggtgaaa ctcaacgtca gcgcgctggc
    27061 caccacgtag acgctcgctc cgattggacg ccgctccgta tacaacaaca gcacgagcgc
    27121 cagcatgatg gacagagagc cccaggaata ggaggtggga accggcagcc agaacagcgc
    27181 cgatgcgctg acgagcccca gcaaggtcaa aacgatcgcg tccggcttcc ggcacccgag
    27241 caaacggaac aggacataga gcgtgccgcc ccatagcccg ccaaccagac tgccgaccag
    27301 gagcacggca cgcagcgggc tgacggacaa tccatgtttg atcagataga ccgggacaaa
    27361 ggtcagcaga gaaaataagg gatgcacgga cgtccggtag tggtcgtcgt gcacgcgggt
    27421 catgttcgag atttcgcgca gcgtatctgc ctcgaaccag aaatccatcg actgaaagag
    27481 aaaggacggc atcgctcgtg cgatcagcag gcaggcgatc gctaccgcca gggaaaagag
    27541 ggcggcacac atccagtctg ttcgatcgag ccccgcagga atggacggcg gcttttgcgc
    27601 cggcggcccc tgcgtctcga cgtgatggtc gaacgcgcga gttttggcgc gaataggctt
    27661 gtgcaaagaa tgagtggccc gtaccagatc cattcgactc ggcatcctgg ttcaaaaaca
    27721 aaacatccct cgcggtttca gcacgctgtt gagggacgaa cgatgtcagc aaacacaacg
    27781 gaaaggcccg tggatcgggc gggaacgatc gatgagacgc ggagtgaggg gagtacgagc
    27841 gaaggagtga ctgtctcatc tcgccgtcga agggatcggc gtatgtccgg tcgactgcac
    27901 aggtgtcgcg tttattccta gcagaccgac cgggtcagat caacccttga tgccacagaa
    27961 aagatgacca acactctttc tgtagattga ggaagcgcag attgcttcag tttcaggcac
    28021 atcgttccac aattggacgg atgagcgtga ttgaggagaa tgaagcgggg ccatgaatgg
    28081 aagctcagtt cgcaggtttc gttaccgcgc atgccaggca tattgggcaa tcagtaccac
    28141 cagcgcaagg cacaatacca ccagcgcggc aagactcgag accagcaccg aacagcctcg
    28201 atactgccgg aagggttccc gacgcatatg cggaaaccgc atgagacaat aggtcgtcac
    28261 cgcggccccg aaaccgacga tggcaatcaa cacctgcgca acgtccattg aaactcctta
    28321 cggaaacacc aggggtaacc ctacccgcga acaggaggag cgtcaagaat cccgacgaaa
    28381 gccgacagtg gtggctgtcc agtcggtata atgagagcca cccatgacag caatcgtcac
    28441 aattggaacc aagcggctgc ggctccggcc atttcaaacg accgacgcag aggccgtgca
    28501 tcgtctcgcc ggatcacaag acgtggctgc cgggactttc cttccacatc caatggatcg
    28561 gcaggcggcc caagactggg tcacggaacg tctgaaggat caggcggcag gaacgggtgt
    28621 aacctttgcc atcaccctgc tggagaacgg cgagctcatc ggctctatcg gcatggagct
    28681 catgctcgta catgaacagg gacgactgac ttattggctg ggccgtgact attggaacca
    28741 aggctacgga actgaagctg tcacggcttt agtcgaatac gggttcaaca gcctgagcct
    28801 gcatcgcatc tacgcgccgc attttcatac gaatccggcg tcgggacgcg tgttacagaa
    28861 ggtgggcatg acatacgaag gccgtctgcg tgagcactat cttcggttcg gccaacgcat
    28921 tgacgtggag ttgtacggaa tgatccggaa cgaatttcta ggactgaaag tcggcgagaa
    28981 aggagtggcc gatgatcatg ggtagattct cgcacagtca gcgaaaagct cacgccgctc
    29041 tggcaagggc tgtgatccgg ctctccttca tagccacagt aggtctaccc aatcttggat
    29101 tcacccaaga agtaaggtcc cccgaccatc gcttctcgct ggaatttgaa acaggtgccg
    29161 tgtggcaatc gcgaaatcag atccagattc ccgataacag caatggaact cgctttggtc
    29221 tgaccgacat tcaagggaac ggccctaacg ttcaacggcg cgttgagctc acgtggaatc
    29281 tcagccatca gcattcactc cgattcgtgt atgcgcctct cgggttttca ggcacagggt
    29341 cgttcggttc cccggtacgt ttttcgggtg gcacgtttgc cccgggtgtt cccatcgatt
    29401 ccgattacaa gttcgattcc taccgcctga cctatcggta tctcgtacac gagtcggaac
    29461 gttggcgctg gcgcatcggc gccacggctt tcatacgaga tgcgcgcgtg gaactgcgtc
    29521 aacagggaag ggtggcgtcc gatagcaacg tcggattcgt cccgctactc agcacgagcc
    29581 tcgagtatga catcgctccg cgctggacgg ctttgcttga cttcgacggg ctcgtcagca
    29641 cacaaggacg tgcgatcgac gcggcgctaa aagtccgcta tgacctcacc gaccattggt
    29701 tcatgactgc gggataccgg gtattcgaag gcggagtgga caatgatcgg tatgccttcg
    29761 gctggtacaa cttcgcgctg gtatcagtgg gcgttcgatt ttagccgtcc gagcaagcac
    29821 tccacctcca gcgccgctcg cagaccgctt tcgacggctc cattcatgta gccctgccaa
    29881 cgcaggctgg tgtgttcgcc ggcaaagaga atccgtccat gcggacggga cagccaggca
    29941 cgttgctccg gatcatatcc gggctgaaac accgcatagc cgccgcgcac gaaacgatcg
    30001 tcttcccagg acacgtgatg cgatgcacac agaacggctt ttgtcgcccc caaccacttc
    30061 agctgctccg ccaccgcctc ggcaccgccg tgggccagga gtcgctgcgt gtcacgactc
    30121 gctgacccgc cggccagcaa ggtgaggatg ccgcttcgcc cccgctgttc ttcgtttccg
    30181 tcccacatcg cgccgatcgg caggtcggtg ccgtacgcca gaggcgctcc ctggtgccgc
    30241 cagaatcgcc gatcgaactg caacagcgtc ttcgtcgcag gtccgtactg gagatcttga
    30301 aaggctttgc gttgtgggga cggcaagggt ggactgaagg cgagccgacg cacgatggtg
    30361 gcaggtagcg ccaggactac cgcatcagcg gtgatgtgat cctgctgacc tcccctgcgc
    30421 acggtaaccc gcctctggcc tcgacgctgc gcaactgcac acacgatggt ctttagttga
    30481 atcggttcgt tgagtgtgtc ggcgagtgcg gtggcgagac gatcgttccc gcctttaatg
    30541 cggtagaaat ggtccaacgc cgacgattcc gcagacagtt gatcgacgag ggccagcaac
    30601 gacagatgct ctgggtcggc cagaaaaaat ccgcgcatgc cattcagccg cgcacgtagc
    30661 tgctggttag ccccgataga gtcgagccag tccgccacag actgttcagc cagctgccgc
    30721 gcgattggcc catcccatcg gcgctcgttc aaccgatgcg ctcgcaccca gggctcagcg
    30781 gcttgtgcca attgagccca gaggtcacct gccttggaag cctgcgtgat gacgggcccg
    30841 cgagccgcct gacgggcgaa ggcaaagccg ccgcgcagga tcggcgatag ggttagcccc
    30901 agatcctttg ccaaccggcg gatggactcc tggtcaggat cgatcatgtc gcccccgccc
    30961 tcggcatgtt gcccctcggc aaaaccgtca cgcctcgtcc acacgcggcc ccccacacgg
    31021 tcgcgcgcct ccaccaccgt gacctgcgca ccgcgccgtt gcagttcgta cgcggcggtc
    31081 agaccggcta gccccgcccc cgcgacgatc actgacatgc cacgcaatga tggactgatt
    31141 cccatgactc tcgacgatac gcgattcagc agctcagaga tagggcgatg tgaggtggcg
    31201 cgacaatcag tcactcctca cgtgaatcca gtacatttct ggaaggatgc gcacgggctg
    31261 cttgatccca cattccagaa tcgctacgct acagtcttga aacgaggagg ttgagcatga
    31321 acgccgatca cccgagtagc ccttcctccc ctgacatgct cgtccccgag ccttcgatga
    31381 attcgacgcc tgctgacgaa cgcagctttc acatcaggct cgccgtcttt gcgtctgtgg
    31441 tcatcattgc ggtggttggc gccttcgtgg gctatccgac gtggcaggaa tacgtctcgc
    31501 agcaggaagc gaaactcgaa ttgcgtgcct acgtcaacgt ccgcgcgttg gggccaccgg
    31561 cgttgaagga aggtgtgtcg cctcgagttc gggagaccat ccagaatgcc ggacggacgc
    31621 cggcctacaa tgatgccatc caggccttca tcaccgtggc cgactacccg ctgatcaaga
    31681 atttcgagcg cgtgacctgc cccccggacg attcgcagcc ggacaaacgc aaatggttca
    31741 tgggcaaggt tccgcgaacg gaaacggtcc gcgagacgcc attcacggcc gaagagatca
    31801 cggccattca gacgaccaag tcagcgctct actaccatgg gaccgtgtgc taccgggaca
    31861 tctttcacga atcgcaccgc accgacttct gtatgttttg gaaatgggat gcaggccgcc
    31921 tgagtccttc gctgtcctgt gatcagggaa acaagacaga ctaggcggga acgccgaaaa
    31981 ccgtaacgtc gcgcaccctt tcactgcccc cagtgacgaa gtccgtcgag aaattcctgg
    32041 ccatactgtg cggcgatcgc ctgctccacg tcccacgtga ccgggccgac tcgcaacaat
    32101 tcggcaactt ctgaaaagta ccgttcaagt cccgcgggcg acaacaccaa cactttccct
    32161 ccgttcacgc cggattgata gagatgcggc aggcctttcg gcacgtacac gaagtcgcct
    32221 gctgccgcct cgtactgttg ctcgccgcaa tgaatggtgt agtggccttc cagcacatag
    32281 aaggcctcgg cgccggtcgg atggatatgc aacgccgggc ccacgcctga tggatgaacc
    32341 atttcaatca gcgaatggga ctcctccgac gaggcaccgg acagtttgac ggtcatcttc
    32401 gtgccgcgaa actcaatcga aggcccttcg cccgacttga gcgcataccc ttgtcgtttc
    32461 catggagtca tgacatcgcc tttcaacctt cgattaccga tcatgtgaat acgtttcaat
    32521 tcatgaggga gcggcaggga aggcggatat ggtcagcacc cacgatatgt ggacttccag
    32581 cggaagcaat cggcaggtca cagcacaaac acgtgatcga cgagatagcg gcctccttgc
    32641 tggaccagaa ccatttcagc gacatagtcg ccaggtgaat cgataaagtg ctgcttccac
    32701 acaatcgcga tggagcctgg acggcggaag acggctaccg gcgttcgcgc cccgaacgtg
    32761 cctttttcgg cctgatactg ccggcacaca cgctcgagat agtctttcgt cacaattgct
    32821 ttcaggcgat cggtgaagtc ctgaacatgg cgagcatggt caatggccgt cgaagcctcc
    32881 atcagattgt ccatgatggg atcggcgacc gctagaattt ccgcgtcgga cttattctcc
    32941 aaatccatct gctcctctta ggaatgtatt cggtgcttag tctcggcgca aagatacgca
    33001 ggcccactga gaagaatcaa ccaggcgatg gacgactatc tgaacaaaac aagagatcag
    33061 aattatggag ccagacctac tccttcacga agcgcactaa ttcctcaagg aacggccgtt
    33121 gcccaacgag cattttctcc tgcgcggtgg gtagatcgaa ccaagcgccg cggtcgactt
    33181 cgggaaactg ttgaattgtt ccggagcgag gcggccattc gagataaaat gagttgctct
    33241 tcaattctgc gggatcaatg tcgccttcta cggcccaggc agagatcacc ttcccgcttg
    33301 gctgcttgag tggcgtgagg gcctgcagcc ggcccgttag ctcaccgccg gtctcttcct
    33361 gaaattcgcg tcgcgcaaca gtctccggct cttcgccttc gatgtactca cctttcggaa
    33421 tcgaccaagc tccgctatct ttcttcgccc agaacggtcc gcctggatga acaagaaaga
    33481 cttgcagccc tccaccgatt ctccgataga gcacaattcc agcgctcagt ttcttgggca
    33541 tacaccacct caaccaaaat atctgaactc ctacctgtcc aggcgttcgt ctgctaccct
    33601 tctctctatg aagcaccaca ctgaaccgga cggcacgctg gttgtaacca ttgactcgcg
    33661 gtttattgtt gggatgctga ccggcgccgc actgctttgc gccgttccgg tctttcggtc
    33721 ctggttgatt ggtggcgcga ccgcgcacga tgccacgccg ctgatcgggt cagccatgtt
    33781 tttggtcggc gcgttcttca gctacgaacg aaatcgtttt gtctttgatc cccatttgaa
    33841 ggaagtgcgg tggagccgcc gtagcctcat ggcaaaggat gaaggccgta tgcctttttc
    33901 atgcctgcgc tcgcttacag tacaagccgc gatcggaagc acgaaaaccg ctcccttagt
    33961 gcggctggcg ctagtgacag acgagaggga aattccgctc tcgagagcct acgcaggcgg
    34021 gatgaggcga gagtatgaag agttggcgat tacgatccgt cgcctgatgc aaatgaacgc
    34081 agaacgaacc aatttcttgc tggacagcct aacagccgct ttggctcaag gccgcaaact
    34141 cgacgcgatc aaactcgttc gcatgacgaa agatatgaca ctggaagaag cacgacaatt
    34201 cgtcgaacag ctagccgtgt ctccgacagc tggctcacga tccaacgatt ctcctccttc
    34261 aagctgatcc tccatggctc atgcaaaggc gatgtgccct acgccgttct caatctcgaa
    34321 tccgaagcct cggacgcctg tgcctcttgc aacgccttca cttctctgag gccaacctgc
    34381 accagggcca gattcacgac ccaggcgatg aagcccaacg caatatactt gagataaaac
    34441 ggcagattga acggcgcgtt ccagaacata tgcaacgcca ccgccaatcc gaagatgcgg
    34501 agaaatcgct tgtcgtacag catctcaatg gtgaaggatc gattccccct gatgcgccag
    34561 agtgcggccc cgaccatgga ggtccataca atatgcaggc ccagcatact caagagaccc
    34621 cgcaccgtga tgctgtgcaa catagccgac tgtccgccgc tcagcccggc catgagggca
    34681 tagccggcgg actcgaacgc ggagaaaccc gtgcccaccg ccgctccaaa caacagaccg
    34741 ttaagcgccg tgcggtattt ttgattgttg atgatgagca ggagcgccaa cgccttcccc
    34801 gtctcctcga tgaccccggc gctcatcgcg cccatccaac tgtcgaggtc cgtccacttg
    34861 aaaaggaaga gcgataaaat cagcgacgtg acgccgccga acatcaccat cttcacgacc
    34921 tgatacagtg aaacattccg aaggacgttc atctcaaaaa agaagatgag cagggacgac
    34981 ggaatgagga acgcacctgt cataatgagc cccggcacga gattggcgtt attgaattcc
    35041 ttcatggcga acacaaacgc cagatagacg accgccgaga gaatgaaggt tttaaaaaag
    35101 acccagggct tcggccagcc ggttatcacc ttttcaatcg gcggtgtggt ggttagcgtt
    35161 cccgccgtga aatgctcttc aatttcctcg tcggtccgct tgcgcagcac gtcggaaaac
    35221 atgtcgcgcg cattgaagcc ttgaagccgc tctacccctg ccacctcgct gatgcgatcg
    35281 ctcagatctt cgatccaccc tccggagcct ctccccggcg tcggcccgtt gagttgccac
    35341 aaggaccgcc ccatccgtat ctgctgctgc agtgtcagaa cggactccat cgtcttgtgt
    35401 ccatcgaggt acacgatgcc ttggaccgcc cggcagatga gttggccgtt ctcccacagc
    35461 acgaccgcat gccgctccac aacatcctga tcgtcgctca gcacatcaca tt
//
LOCUS       DAOQEW010000014        34879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_254_length_34879_cov_8.543820, whole genome shotgun sequence.
ACCESSION   DAOQEW010000014 DAOQEW010000000
VERSION     DAOQEW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_254_length_34879_cov_8.543820"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLS35_02935"
     CDS             complement(<1..607)
                     /locus_tag="PLS35_02935"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNI17637.1"
                     /translation="MARRIGVMGGGAVCVLVMGLVCGVSVHARAEGTEAAVPGQDASA
                     AKSNEEASGVVAESGETPKPKPVKIPEVMVRDVHERNEPDQESVKDIAGSVNVVTREE
                     IQRARPKNADEMLRRVPGVNVLDEYGQGLRPNIGIRGMDPRRSKNILLMMDDIPIQPA
                     LFGDASTYYMVPIERIDHIEVIKGGASVLYSPNTQGGLINFI"
     gene            complement(749..937)
                     /locus_tag="PLS35_02940"
     CDS             complement(749..937)
                     /locus_tag="PLS35_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17638.1"
                     /translation="MTNSTKREPARMLTCDDVRCRCGQLVARWAEEGIEIKCKRCRRL
                     VTIAFGSIAGRPPAMLEN"
     gene            complement(994..1452)
                     /locus_tag="PLS35_02945"
     CDS             complement(994..1452)
                     /locus_tag="PLS35_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17639.1"
                     /translation="MKDVMQRVRRGLSSAVLCSVCLLWLGTGTALAESKGEKAFQAVA
                     TDAKGVETDVHGIIFYWEEKISETSFVPHELKEVPVKRGTATVKIKFDVIKAIDVKPS
                     GNGAPPAVSITLNDGKTGEFTLAIAGSFKGQSDFGEVELPAVELKRIAFK"
     gene            complement(1492..2535)
                     /locus_tag="PLS35_02950"
     CDS             complement(1492..2535)
                     /locus_tag="PLS35_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNI17640.1"
                     /translation="MSDIALRAHIVYERGSRSGGWLISLLLHGGLVALAMQAVMEVTP
                     HRQTETFRWDVALQSAPAPSPVAAEPLPPQQSVPQPPQPQKPVSRPVSQPSPEPVSQV
                     VERTAVVQTVQATQVTPRVVERSAPQERQAVATEVQPVTSQPVHETMVAHAQEILPQE
                     RQETVHTVEAQAVVTQVSEAMIQESAVPVATQSTAVEQPPIVEPAVVATEAPVETSAA
                     PVIEPQEAIVSEPVVVRQSAVVHREVREQAPAQADLGWLAESLWSRIEELKRYPRQAR
                     ARRWEGKVVLEAVIRHDGTILECLIAESSGHGLLDQDAISVLRKASPLTLKHPLGKEQ
                     ITILVPIAYRLES"
     gene            complement(2823..5924)
                     /locus_tag="PLS35_02955"
     CDS             complement(2823..5924)
                     /locus_tag="PLS35_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNI17641.1"
                     /translation="MNISAPFVRRPVATTLLTIGATFIGIVAFQFLPVAALPQVEFPT
                     INVSASLPGASPEIMASSVAAPLERQFTRIAGVTEMTSSSTMGGTNVTLQFELDRDID
                     GAARDVQAAINASRVDLPPNLPSAPRYSIVNPADGPILILAITSELVTRGQMYDAASS
                     ILQQKLSQVEGVGQVAVGGGSLPAVRVDLNPTALNKYGIGLGDVREVLTSTNVNRPKG
                     QLTSDDRTWQIRTNDQLHKADEYLPLIVAYRNGRAVHLSDVASVESSVEDLRTMGVAN
                     GTPAVLVIVYRQPDANIIETVDRLRARLPQLEASLPGSMSLSIVMDRTPVIRASLHDV
                     ERTLAISVALVILVVFVFLRNLRATLIPAVAVPVSLISTFGVMYLLDYSLDNLSLMAL
                     TIATGFVVDDAIVVLENITRYREQGVPAMEAALRGAKDIAFTVVSMTVSLVAVFIPVF
                     LMGGMLGRLFREFAVTLSVAIGVSLLVSLTTIPMLCARVLKSETNVAHGWWYRISERG
                     FNVMLRGYATSLGWVLRHPRTMLIVTLATMALTIYLYVLVPKGFFPQQDTGRLFGNIR
                     AAQDISFQAMRDKLTEVVGIIKSDPAVASITGFTGGGSHSGTTNTGRMFIALKPLEER
                     GMSSEEVIARLRPKLAKVPGAPTYLQAIQDLRIGGRASSAQYQYTLQSVDLEELNTWA
                     PKVENKLRTLPEIVDVNSDQQDRGQQSLVIFDRSTASRLGLTPQLIDDTLYDAFGQRQ
                     VSIMYTPLNQYHVVMEVAPQYWQNPDALHEIYVRSPNGAQVPLSAVTRYEPANAILSV
                     NHQGQFPAVTVSFNMAAGVSLGEAVAAIDQAMHEIGLPASVRGTFQGTAKAFQSSVEN
                     QPWLILAALVTVYIVLGILYESYIHPLTILSTLPSAGVGALLALLLFKSELSMIALIG
                     IMLLIGIVKKNAIMMIDFALQSERLGEGKTPQAAIYEACLLRFRPIMMTTAAALLGAL
                     PLAVGMGVGSELRRPLGIAIVGGLLVSQVLTLYTTPVVYLLLDRLRLRWVRARESAPH
                     PAA"
     gene            complement(5921..9034)
                     /locus_tag="PLS35_02960"
     CDS             complement(5921..9034)
                     /locus_tag="PLS35_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNI17642.1"
                     /translation="MNPSRLFIVRPVATVLTMIAILLAGALAYRHLPVSALPQVDYPT
                     IQVLTFYPGAGPDVMTSSITAPLERQFGQMPGLNQMTSTSSGGSSVVTLQFSLDIDLD
                     IAEQEVQAAMNSAATFLPRDLPAPPVYNKVNPADAPILTLALTSATLPLSQVRDFAET
                     RFAQKISQLSGVGLVSISGGQRPAVRIQANPRALAAYGLTLEDLRVVVAAANVNQAKG
                     GFDGPRRASIINATDQLFAGKDYKALIVAYRNGAPVRLSEVAEVIDDVENTKQAAWMN
                     ETPAVIVNIQRQPGTNVIEVVDRVKQLLPQLQGTLPSSVQVSVLTDRTTSIRATVRDV
                     QFELVLAVALVILVIFLFLRTLPATVIPAVAVPLSLIGTFGLMYLMGFSLNNLTLMAL
                     TISTGFVVDDAIVMIENIARYIEQGESPFQAALKGSKQIAFTILSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLSITILISAIVSLTLTPMMCARLLRERREEHPGPLARASQQM
                     LDRIIAGYGRSLQWVLRRQQATLLVTGATLVVTVALFVLIPKGFFPLQDTGVILGITE
                     GPQAVSFGTMAERQQALARVILEEPAVESLTSFIGVDGTNTTLNSGRVYINLKPLGER
                     VGGAGDIIRRLESRVADVEGITLHMQPVQDLTVEDRVSRTQFQYTLEDADPEELNRWA
                     PKLLEHLQLRPELRDVSSDQLNQGLASLVEIDRNTASRMGITPQLITDTLYDAFGQRQ
                     VSTMFTQLNQYRVILEVMPEFQKGPEALQALEVRGSGGSVPLNVFTRVTETTVPLVIS
                     RQGQFPAVTISFNLAPNGSLGAAVEAIRHVTQELGLPASIRGSFQGAAQAFQNSLEHE
                     PLLILAALITVYIVLGILYESYIHPLTILSTLPSAGVGALLALMLFRMEFSIIALIGI
                     VLLIGIVKKNAIMMIDFALEAERNEGKPPEQAIYEAGLLRFRPIMMTTMAALFGALPL
                     AMGAGVGSELRRPLGIAIVGGLVLSQLLTLYTTPVVYLALDRVATRFRRSREPVSVAG
                     ALPIGPP"
     gene            complement(9031..10269)
                     /locus_tag="PLS35_02965"
     CDS             complement(9031..10269)
                     /locus_tag="PLS35_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HNI17643.1"
                     /translation="MAESVRFSTLLKRWMIGLSAACLLAVGTYVTMTRSGEAQPSRSE
                     KRPPTAARNTPVVAAVAKTGEINIYLNGLGSVIPMNTVTVKSRVDGQLMRVLFKEGQL
                     VRNGELLAQIDPRPFEVQLIQAQGQMARDQAQMKNAQLDFERYRDLYKQNFIPKQQLD
                     TQEALVRQYEGIVKADQGQIDNAKLQLTYSSITAPIDGRVGLRLVDAGNIVHANDPNG
                     LLVITQLQPITVVFALAEDHLPAVFERLKSGKQLVVEAFDREQKRKLATGTLLTVDNQ
                     IDPTTGTVRLKAVFPNDDSALFPNQFVNARLLLDIKQGATIVPSAAIQRGPKGTFVYV
                     VNEAEQTVSVRAVTVGVSQGEDTSIDTGLASGETVVVDGTEKLREGSKVEMRPSGGAV
                     PTSQESPPAGGERKKQGKRT"
     gene            complement(10269..>10655)
                     /locus_tag="PLS35_02970"
     CDS             complement(10269..>10655)
                     /locus_tag="PLS35_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17644.1"
                     /translation="CAGALMGAVGTSLYYQYEDQHRWERGPGARQERIMKRLTQELVL
                     STTQQAAVEPIVARAHVELLRLRVQHQPDVDRVLDQGMQELKATLSPEQQAKLDGLHA
                     QLQQRWRMTREYVRQAQDAGGRKEER"
     assembly_gap    10656..10755
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10990..11898)
                     /gene="cynR"
                     /locus_tag="PLS35_02975"
     CDS             complement(10990..11898)
                     /gene="cynR"
                     /locus_tag="PLS35_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HNI17645.1"
                     /translation="MEFRHLRYFLAVADALHFTKAAEGLPVSQPALSAQIKQLEQEVG
                     VPLFDRVGRSVQLTRAGSIFREHARRALREMELAQVAIAEEEGLQRGTLTVGVVQTVN
                     AYLVPEIVGRFSTLHPHVSLKFDELSGPDIEAGVKNGVLELGIGFVPPGSDRIATQPL
                     FEEDFVLIASPRHRFATRRHLPLASLADEPLVLLPGIFCTRRLLNASFEQAGIVPRVT
                     VEMNSIEGILATVKTSKLATVLPRLSLGLERHDGLRGIPLNNPTPRRALGLLWKKGGY
                     RSGAARALADQVRLVVNKRWKQKPGK"
     gene            12025..12744
                     /locus_tag="PLS35_02980"
     CDS             12025..12744
                     /locus_tag="PLS35_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNI17646.1"
                     /translation="MQQVSGFIRSIQVGLPEQGHPHCHEDRPPQSWSTGIFKRPVRGP
                     IRLQQKNLEGDGQADLIHHGGVDKAVCVYPSEHWFHWSSVLPQQQLIGGEFGENFTLE
                     GLTEADVCIGDIFSVGSAVVQISQPRQPCWKLARRWQIKDLAVQVEQTGFTGWYFRVL
                     QEGVVESRIPLHLTDRPYPEWTVTTANRIMHQEHSNLKAAEHLSLCPLLSASWRHALH
                     QRSQQQNSPDPQPRQFGNSQS"
     gene            12768..13106
                     /locus_tag="PLS35_02985"
     CDS             12768..13106
                     /locus_tag="PLS35_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17647.1"
                     /translation="MTRLTLSVCSLALGAILAMSSWCAAENVTTINILSPRNGDSVPE
                     TFEIKYEILNAPEGNHAHVYLDGAYQKGFKGTFTHVPKGEHTITVKIADHDHKDAGTA
                     TDSVTVKVGE"
     gene            complement(13214..13897)
                     /locus_tag="PLS35_02990"
     CDS             complement(13214..13897)
                     /locus_tag="PLS35_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17648.1"
                     /translation="MCECINRKMFQAPLRHLAAAGLVGVLFTTLLPAPLNAARGGTSP
                     GGGKTEILPVQKAAPSVVIIDASGSEADLRRQLRSKNGVAELGASTSKTMFSQAPTGT
                     FGFILPKFLGLALVTQSPDLVLERVSPATSAYEVHKLADGSGLLIGFMGKDVLMQVAP
                     SQRPKSVRIALHSNPSEKAPHIVAVPLVKLAADMMPLRIDPKNPDSPVVFEMDLQSSS
                     TRASTQGGQ"
     gene            complement(13992..14432)
                     /locus_tag="PLS35_02995"
     CDS             complement(13992..14432)
                     /locus_tag="PLS35_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17649.1"
                     /translation="MGMAWPFGLSTGDKVSLGRVLVVDDEDSIRSVLRMMLTQAGYDV
                     EEADDGGKAVEVLNSGDNPLMLDAIICDIRMPRINGVEAIAYFRAQYPSLPVIVLTGY
                     HDEPLASSLRRQGIVEFLEKPAEREQILASVAQAIAGRRISFYP"
     gene            complement(14762..14941)
                     /locus_tag="PLS35_03000"
     CDS             complement(14762..14941)
                     /locus_tag="PLS35_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HNI17650.1"
                     /translation="MFLKWAAVFFVLAMVAAAFGFTGIADKASDVAEILFYIFLAIVL
                     TFVVSGVVVARKLTA"
     gene            complement(14984..16345)
                     /locus_tag="PLS35_03005"
     CDS             complement(14984..16345)
                     /locus_tag="PLS35_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI17651.1"
                     /translation="MSRSTTPLEIESHEQDNIDLCPSARIRPPKVVHLSTYDVAGGAA
                     RAAYRLHDGLRRAGVDSSMFVREARTSDGSIIRFEPTTTFCKRAERRIRREQIERKLR
                     PAISPENDKIEPFRTDRSEYGTDILSKLPPYDILNLHWIAEFVDYPSFFGAFSKNTRV
                     VWTLHDMNAFTGGCHYDLGCGRYLGECGDCPQLAAAGPNDLSHEIWARKKSLFDQLSD
                     GQIHFATPSTWLAEEVQRSPILGRFPVSVIPYGLDLTEFSPRDVGSAREVFGIPPKAK
                     VILFVADGLPLFRKGFEVLVRALEHIRSWIPDLHLVTVGHNSPDLGHHIPHSYLGHIN
                     SDRLLSNIYSAADLLVIPSLQDNLPNTVLESMACGTPAVGFEVGGIPDMIQHCETGLL
                     VPPGEAGALGAAMLELLQNPERRLALGANCRQYALNHFSLEEQADRYCQLYTEILSER
                     AIR"
     gene            complement(16489..17781)
                     /locus_tag="PLS35_03010"
     CDS             complement(16489..17781)
                     /locus_tag="PLS35_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17652.1"
                     /translation="MGNVAISVDRLTKRYSLGLQARHDTLRDRLAAALSWWKQCRSDD
                     TGKGREFCALTDVSFDVKPGEVFGIIGHNGAGKSTLLKILSRITAPTSGTADIYGRVG
                     SLLEVGTGFHPELTGRENIYLNGSILGMRRAEITAKLDEIIDFAGVEEFIDTPVKRYS
                     SGMYVRLAFSVAAHFEPDILIVDEVLAVGDAGFQQKCLGKMRQASRGGRTVLVVSHNL
                     PIIENLCQRALFLAQGRVALTGTARDVVQAYAGMTADLARSALDTRTDRQGKGHVIAT
                     AIEVLDQYRNVSSSAICGRETIVRWHYRCVPDKIIRRCRVELSLHARIGDPYETAYFL
                     MSTDLVSPLPLDLSGDGYIDFRVPEWPLSGGQYYVMSYVESDKEVQDWVHNASLLSVV
                     DGDFYGTGKSYPPNWRGKSVLVKYDWTQTAREAVHSFL"
     gene            complement(17910..18275)
                     /locus_tag="PLS35_03015"
     CDS             complement(17910..18275)
                     /locus_tag="PLS35_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17653.1"
                     /translation="MMSQRLSNWSRRGWMVVMLAVGALTAGCMHHPGGIAPSTKPLAP
                     GGYTELGKVRGQDCVYHLLGLIPVTGGNEMRNAVEDALRTKPLADAMVEVTVDGYFQY
                     FILFSRACTQVYGTAVETK"
     gene            18754..19818
                     /locus_tag="PLS35_03020"
     CDS             18754..19818
                     /locus_tag="PLS35_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNI17654.1"
                     /translation="MITASGAVILILIGLLLGLGEVIIRSVHLIRDGIPFFESNAGGR
                     IGPITLDQDLGWKATEHYQEALVEKTKNGTLYPVRRSQTQYGFRSFGDVSSRRPKMLV
                     IGDSFTHATAVSDDRTYHALLSKLLDVEVFAYGAGGYGTLQEYLVFDRYLDIVRPDVI
                     LWQYCINDFINNDNELERLSRVNNNGWVRPYWRNGTIQLLSPKESSLQIRDWINRHSR
                     FLYFVVTRLDRLRAANTRDTVEVDIEAEGMRHAGFARSVAVTDDLMGRVRARAGTRPI
                     MAFNCTEAEPYSQAFRDISGHHAIAYWGDVAQVVQDADTRGEDVFAADGSHWNERGHE
                     LAAQALAKHFKTALPPVPRP"
     gene            complement(19879..20151)
                     /locus_tag="PLS35_03025"
     CDS             complement(19879..20151)
                     /locus_tag="PLS35_03025"
                     /inference="COORDINATES: protein motif:HMM:NF040444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HNI17655.1"
                     /translation="MPEQKTMQRARQAKREGKAPTTQAGAFVEEEMEHMKRGKHRVQS
                     RKQAIAIGLSKARKAGVKVPAAGSKRATSASRRKSASRTRGSKRRE"
     gene            complement(20144..21247)
                     /locus_tag="PLS35_03030"
     CDS             complement(20144..21247)
                     /locus_tag="PLS35_03030"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI17656.1"
                     /translation="MKAHRHHHPRVVVITGASAGVGRATVREFAREGAWIGLVARSRD
                     GLEGARKEVEAAGGRALVLPTDVAYPDQVEAAAAMVERRFGPIDVWVNNAMASVLSPA
                     LKMTAEEYQRVTDVTYLGYVYGTLAALRRMVPRDVGVMVQVGSALAYRSIPLQSAYCG
                     AKHAVQGFVDSLRSELLYDGSRVHITTVQLPAVNTPQFDWIKSRMPCHPQPVPPIYQP
                     EVIARAIVWASRHRRREISIGLPTVKAIVGQKFIPGLLDYYLAWKGYKAQQTNEPIAP
                     DRPNNLFHPLPGDHGAHGGFDEGAYEFSLQLWLNQRRLWVAAAALAVGGYLAKGLSTR
                     RNDRRWDGEPFSPTGGTTRSAREQAARQGGLYA"
     gene            complement(21370..22419)
                     /gene="cydB"
                     /locus_tag="PLS35_03035"
     CDS             complement(21370..22419)
                     /gene="cydB"
                     /locus_tag="PLS35_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNI17657.1"
                     /translation="METLWFSALTFVLAMYIVLDGYDLGVAMLFPVVATDESERRMVR
                     ATIGPVWTGNEVWLIAGSGLLFLSFPKAYAAGFSGFYLGFIMVLWLLMGRGLAFELRG
                     HVEHPLWRRFWDTIFFVTSLSLAFLLGLFAGNVIRGVPLDADGYFFLPLWTDLRPGLN
                     PGILDWFTLLAGLTMVALLTLHGASYLAMKTVGRLQARSRALASWALLPAVLLVLVFV
                     VAIPFVRATFTVNFHTHPAGYLWVVLGLLAFVSLALSHTRQRQVAAFASSSVLLAVFI
                     AVIAWGTYPNLLVATTEPANSVTIHNASPGKDGLQAALWWLPFGLLVVVLYQIQIHRM
                     FAAPVTPDSRSVSDH"
     gene            complement(22419..>23708)
                     /locus_tag="PLS35_03040"
     CDS             complement(22419..>23708)
                     /locus_tag="PLS35_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI17658.1"
                     /translation="FPQLTMGLALFVLYFRTRDLFSVGEHFHEVARFWTGIFALSFAF
                     GVVTGIPLEFQFGTNWAAFSNYAGGIIGQTLAMEGVFAFFLESSFLGVLLFGESRCSR
                     RILWVASAMLWVGSWLSGYFILATNAWMQHPVAYRVLDNGKLAIESLAELLTNPWLLW
                     QFTHNMTAAVATGSFVMAAVGALYLLAGRHLVHAKTYVQTGVVAGAVASALLITPTGH
                     ESARQVFEHQPIKGAAFEGLFRSERGADILLIGQPNLETMTIDNPLVVPSALSLLVYY
                     DLYANVKGLDAFPPQDWPDHIALLYFSYHIMAGLGTWLVVVMGLAFLWLWRGRLFASS
                     WLLWLLLLSAPAPYIATTAGWMTAELGRQPWLVYGLFRTAEGISPLVHSGNALFTLLG
                     FLGLYLALGLLFVLLFLEMLRRGPIDHGATAGQSGEL"
     assembly_gap    23709..23808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23809..24712
                     /locus_tag="PLS35_03045"
     CDS             <23809..24712
                     /locus_tag="PLS35_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNI17659.1"
                     /translation="SDDASHERLAQTLGWFSIGLGMAELFAPRTVARIAGLPHDHRWL
                     LGASGLREITMGLGILSERRPAGWMWSRVGGDALDLALLATALSSAQGNRKRTAAAML
                     AVAGIAAVDCFASRHLSRMVGWTSDDGAVLIKKTIMIDSSPEALYLFWRDFQNLSRFT
                     EHVESVTIYDRHHSHWTVKGPAGYRVEWNAEITDDEPNRKIAWRTTSQADIHHTGSVE
                     FTPSPGGRGTLVRVVMEYNVPAGVLGASIAKLFGSEPGQQVQSDLRRLKQIIEVGEVL
                     RSDGSLHGKGILQQRPAQPAGSHT"
     gene            24763..25929
                     /locus_tag="PLS35_03050"
     CDS             24763..25929
                     /locus_tag="PLS35_03050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HNI17660.1"
                     /translation="MKANCWYGAGDVRVEQVPDPTILNRRDAILKVTSTAICGSDLHL
                     YNGLMPTMKAGDILGHEFMGEVVEVGSEVTNLRVGDRVVTAFPIACGRCLPCRQELFS
                     LCENSNPNAWMAEKLLGHSPSGIFGYSHMLGGFAGGQAEYARVPFADVGTIKIPSDIP
                     DEQVLFLSDIFPTGYMAAENCQIQSGDTIAVWGCGPVGQFAIRSAFLLGAERVIAIDR
                     FPDRLQMARDGGADTMNYEEEEVYDRLMEMTGGRGPDACIDAVGMEAHGPGVLAAYDR
                     MKQAVMLESDRPYALRQAIMSCKNGGTVSIIGVYSGFIDKFPIGSIMNRSLTIKTGQA
                     HVQRYMAPLLERIQRGEIDPSVIITHRMRLDEAPQAFAKFNRKEDHCMKIVLTP"
     gene            complement(25948..26130)
                     /locus_tag="PLS35_03055"
     CDS             complement(25948..26130)
                     /locus_tag="PLS35_03055"
                     /inference="COORDINATES: protein motif:HMM:NF040444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HNI17661.1"
                     /translation="MARYSKGSQKTVQEAMHKRKKGTLKSGSGKKVKSKKQAIAIGLS
                     EARKKGKKVPAKKSAK"
     gene            complement(26182..26616)
                     /locus_tag="PLS35_03060"
     CDS             complement(26182..26616)
                     /locus_tag="PLS35_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI17662.1"
                     /translation="MPLLKEIMTPSVEVIAPNATAADAARKMRDLDVGAIPVCDGEKL
                     LGMVTDRDLVLRVLALSRSPVDALVSDAMTPGLVYCFEDQDAEVAAELMAEKQIRRIP
                     ILSKNKQLVGIVSLGDLAIDGLDQHTSGEVIHDVSDPSRGGH"
     gene            complement(26716..27057)
                     /locus_tag="PLS35_03065"
     CDS             complement(26716..27057)
                     /locus_tag="PLS35_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17663.1"
                     /translation="MLPCHPAHNIQAAARRRLSLKEKRGLMAGLAAKARDKLGRLRLG
                     RARQVQGELKAVKEAQGEGHLPQGPGWGRGPAWEVGQEWAPEAGEARVAAVLEVAAGG
                     NAQRWATEGQN"
     gene            complement(27179..27685)
                     /locus_tag="PLS35_03070"
     CDS             complement(27179..27685)
                     /locus_tag="PLS35_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI17664.1"
                     /translation="MAGKTAFASGVVEMLKEDHAKVKGLFEEFESADGQEQAEIAATA
                     IFELEIHAELEEKLIYPAIREAIDDSDMMNEAVEEHHLVHVLIKELKKLKPKDEKFHA
                     KFSVLSELVKHHIEEEEGEMLPEAERHDIDWDALEVAVMKRKEVLMAKSSKKSGSRSK
                     ATPKSGRS"
     gene            complement(27840..28316)
                     /locus_tag="PLS35_03075"
     CDS             complement(27840..28316)
                     /locus_tag="PLS35_03075"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HNI17665.1"
                     /translation="MAMLKSHSAPNLPQPEAASEVASHLLEECRMVLPGIQALFGFQL
                     IAVFNNKFGEMPEAHQTLHLLAIVFVTISIAFVMTPAAYHRQAHQFAISRTFVQLSSL
                     LLLLSMILLSVGICLDVYVVCALITGSDAISLMVALAAALLFALLWFVFPRCIRNP"
     gene            complement(28332..28727)
                     /locus_tag="PLS35_03080"
     CDS             complement(28332..28727)
                     /locus_tag="PLS35_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI17666.1"
                     /translation="MSISSSRIRTVHHESGFIRSFTLESSEQFEADAVFTTRGDVVHN
                     RLAAQAGAALNEEGQVVVDHCMQTSVPGLYAAGCLTPANCQMIIAAGQGATAGQAINR
                     DLFEESLRRHALPMRASTRDCRESHRLMC"
     gene            complement(28724..29305)
                     /locus_tag="PLS35_03085"
     CDS             complement(28724..29305)
                     /locus_tag="PLS35_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNI17667.1"
                     /translation="MNRDAKGWDVIIVGSGPAGLSAALYLGRSRRRTLLLHSGHSMAK
                     WEVNVQNYLGFPQGIAGTDLLERGLTQAMQFGVEVIEDTVVSLSVGDPFQLEGGKTRY
                     EAGRILLATGLTHVPPDIPGVKECLGHSLFFCKDCDAYRVQGQRIVIIGWNNEAAEYA
                     LGMLTFSSNVKVCTNGRPAAWIRPMPRGSTSMA"
     gene            complement(29364..29939)
                     /locus_tag="PLS35_03090"
     CDS             complement(29364..29939)
                     /locus_tag="PLS35_03090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNI17668.1"
                     /translation="MALLIIDMINPFTFPRAEDLFPAAYDVAVRICRLKQKANQAAMP
                     VIYINDNFGTWRHDFQTLVRQCRDEPCRGRGIAELLAPSQNDYFVLKPKHSAFFATPL
                     EILLQSLSVRRLILTGVTGDQCILYTAADAYMRGYGLFVPSDCTASLSAQVNEQALEH
                     MRTYFDANTCLSEQLITTAPAEALAKCGTPQ"
     gene            complement(30125..30580)
                     /locus_tag="PLS35_03095"
     CDS             complement(30125..30580)
                     /locus_tag="PLS35_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17669.1"
                     /translation="MNIGVYVRLAHHSETQLAEAFLQVSDHHRAEPDVQETCRLLSSW
                     SKEKSNGIRPFIATYGEPRVNEPETLFSDLFQGPRTGGLALLRDLHDLWLLATEAMIS
                     WTVLLQASRALRDEDLEHLCTRHITQTDRQLAWLQTRTKQAAPQALVVA"
     gene            complement(30577..32925)
                     /locus_tag="PLS35_03100"
     CDS             complement(30577..32925)
                     /locus_tag="PLS35_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HNI17670.1"
                     /translation="MTITRNSIEDIWGPRTPYRGQWPERSDCNVEGTPEKWVPSACVL
                     CSNGCGVEIGVREGKIVGIRGRSDDRVNRGRLGPKGLYGWKANASPDRLTHPLIQKKG
                     RFEEVSWDEAMDVIVRKSQEVRQQYTAGAIGFYTSGQLFLEEYYTLGVIGKAGLGTPH
                     MDGNTRLCTATSSAALKETFGCDGQPGSYNDIDTTDALFNFGHNVAEQQTVLWMRMLD
                     RLCGANPPKLVVVDPRPVPAALKADVHLAPKPGTNVAIMNGLLHLLIKHGWVDYSYLS
                     AHTMGFEQLEKVVAAWTPARVAQVADIPERGLREAAEILGTTPTLVSTVLQGVYQSMQ
                     ATAAACQVNNLHLLRGLLGRDGCGIYQMNGQPTAQNTRECGADGDLPGFRNWDNPSHI
                     ADLARLWNVEPHIIPHWAPPTHAMQIFRYCEQGSIKFLWISGTNPAVSLPDLRRTRRI
                     LGQKDLFVVVQDAFMTETASRADVILPAAIWGEKTGCFTNVDRTVHLSRKAIEPPGEA
                     RPDLEIFLDYAERMNFRDKDDQPLIKWHTAEEAFEAWKACSRGRPCDYSGMTYERLSS
                     RSGIQWPCNSENPDGTSHLYVDGHFPSDAEYCETYGHDFDSGAAATPERYRAHNPQGR
                     AVIKASDYLAPFEEPDERFPLWLTTGRVVYQFHTRTKTGRVEELQAKAPEPFVEISSQ
                     DAADHGIADGERVEVRSRRGYVQMRAKITGIRPGVLFIPFHYGYWDENSHPRAANELT
                     LVEWDPVSKQPHFKCAAVRIGKPGKSGLVPKLVKTFVRKTFG"
     gene            complement(33021..34061)
                     /locus_tag="PLS35_03105"
     CDS             complement(33021..34061)
                     /locus_tag="PLS35_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI17671.1"
                     /translation="MVDVSCLICGGVYHETVAFAAEIAEQKELLRRFHQQRQRQDAQA
                     DLKDRTTFSQDYSTEVVRCLTCGFVYRNPRPTLRSVTAQYADDQYGEEHLEAEFQAQQ
                     RWAEWKITAFAQHCHFTSRHPVVVEVGSFVGGFLAAAQQRGWSILGVDPGKEVVNFCQ
                     ARGLPVFHGTLNDATIAARSVDAVVIWNTFDQLVNPDTTLSAARRLLREDGHLVLRVP
                     NGAMFQQGVHWLKAGGSRRRWAIVTLAWNNLLSFPYLNGYSVSTLDQLVARHGFSRVA
                     VEVDTLMTLSDQHTTLWGQWEERVVKSSCRILAGTTTLLPSLHNMVSPWLDVYYAVSG
                     DAATSPPIACPA"
BASE COUNT         7567 a        10541 c         9531 g         7040 t
ORIGIN      
        1 ggatgaaatt aatcaggcct ccctgcgtgt tcggtgaata gagcacggac gcgccgcctt
       61 tgatcacctc gatatggtcg atacgttcga tcggcaccat gtaatacgtc gaggcatcac
      121 caaagagagc cggctggatg ggaatatcgt ccatcatcag cagaatattt ttgctgcgcc
      181 gaggatccat cccccggata ccgatgttcg gccgcaaccc ctggccatat tcgtccaaca
      241 cgttcacacc gggcacgcgt cgcaacatct cgtctgcgtt tttcggccgg gcccgctgaa
      301 tttcctcgcg cgtgacgaca ttgaccgacc ccgcgatgtc cttcaccgac tcttgatccg
      361 gctcgtttcg ttcatggaca tctctcacca tcacttcggg aatcttcacc ggcttaggtt
      421 tcggcgtttc ccccgattca gccacaacgc ctgaggcctc ttcattggac ttggcagcag
      481 aggcatcctg accaggcaca gccgcctctg ttccttcggc ccgcgcatgg acactcacgc
      541 cgcagacaag tcccataacc aacacacata ccgcccctcc gcccatgacc ccgatacgtc
      601 tcgccatgat tacttgttct ccttgttcat tcaccgacac gtagaaagac atcagcacac
      661 ataaaaaagc ccaagaccct gttgatggtc ttgggctctg gacacacacg gtcctttggc
      721 ctctatggca tgatcggctg aggatggatt aattctcgag catcgccggc ggcctccccg
      781 cgatcgaccc aaaagcaatg gtgaccagac gccggcaccg cttgcatttg atttcaatgc
      841 cctcttccgc ccaacgagcc accagctgtc cgcaccggca ccgcacatcg tcgcacgtca
      901 acatccgggc aggttcacgc ttcgtgctgt tcgtcatgcc aattcctcca ctcgacggcc
      961 ttctgttccg tcacgctcaa ttcgccgccg catttattta aaggcgattc gtttgagttc
     1021 gaccgccggc aattcgactt cgccaaaatc cgattgcccc ttaaagctgc cggcgatcgc
     1081 cagggtgaac tcgccggtct tgccgtcgtt caacgtaatc gagacagccg gcggcgcccc
     1141 gtttccggac ggtttcacat cgatggcctt gatcacatcg aacttgattt tgactgtggc
     1201 cgttccgcgc ttcaccggca cttccttcag ttcgtgcggc acaaacgagg tttcgctgat
     1261 cttctcttcc caataaaaaa taatgccgtg cacatcggtc tccacccctt tggcatcagt
     1321 cgccaccgcc tgaaacgctt tctcgccttt cgactcagca agagctgtcc cagtccccag
     1381 ccataacagg catacgctgc acaggacagc gctcgacaac cccctccgca ctctctgcat
     1441 gacatccttc atggtgaaac tcctttgccc cttgtgatga tgttgctgtt actaactttc
     1501 aagccgatag gcaatcggca cgagaatggt gatttgctct ttccccagcg gatgtttcaa
     1561 ggtgagggga gacgccttcc gcaacaccga gatggcatcc tgatccagca acccgtgacc
     1621 ggaactttcg gcaatcaaac attccagaat tgttccgtca tggcggatca ccgcctccag
     1681 cacgaccttg ccctcccacc ggcgtgcccg cgcctgccgg ggatatcgct ttaattcttc
     1741 aatcctggac cacagcgact ccgctaacca gccaaggtct gcctgcgccg gagcctgttc
     1801 acgcacttcg cgatgcacga ctgcgctttg acgcacgacg accggctccg atacgatcgc
     1861 ctcctgcggc tcgataacgg gagccgctga ggtctccaca ggtgcttcag tcgcaacgac
     1921 cgctggctcg acgataggcg gctgttccac tgcagtcgac tgcgttgcga caggaaccgc
     1981 cgactcttga atcatcgcct cggatacctg cgtcaccacg gcctgtgctt cgacggtatg
     2041 aacagtctcc tgacgttctt ggggaaggat ttcttgcgca tgcgccacca tggtttcatg
     2101 taccggttgc gaggtgacag gctgtacctc tgtcgcgacc gcctgacgtt cctgaggagc
     2161 ggagcgttcg actactcgcg gggtgacttg cgtagcctga accgtttgaa cgactgccgt
     2221 ccgctccacg acctgagata ctggctccgg tgaaggctgc gacaccggcc gagacacggg
     2281 cttttggggc tgtggcggtt gcggaacgga ctgttgcgga gggaggggct cagctgctac
     2341 aggactcggc gctggggccg actgcaaggc gacatcccat cgaaaggtct cggtttgccg
     2401 atggggtgtg acctccatca ccgcctgcat cgcgagggcg acaagtccgc catgcaacag
     2461 caatgagatc agccatccac cgctacgaga tccccgctcg tatacgatat gggctctgag
     2521 tgcgatgtct gacatgctcc gacctcctcc ccgaccagac aatccggtcc gcgggacagt
     2581 aaataaaaaa agcccaaagc tgtcgtgata cagctttggg ctctgaccgc aggggtcgct
     2641 ggccttattc gagtgttgcg cgaacgtgac gcactacccg cgtccttgac caagatttag
     2701 tcggcttttc tcccgacaag tcttgatagc aattctcaat atcatgaccg cgaaacagtt
     2761 gtcaatgcgc atatcgccct actcacggcg gcgaagcact tccatgtata tcgtgcgagt
     2821 ccctatgcgg cgggatgagg agcgctctct cgggcgcgga cccatcgcag tcgcagacgg
     2881 tcgaggagca ggtagacaac aggagtggtg tagagggtga gaacctgact caccagcaac
     2941 ccgcctacga tggcaatacc gagcgggcga cgcagttccg atcccacccc catgccaaca
     3001 gccagaggca acgcacccaa gagggcggcc gcggtggtca tcatgatcgg tcggaaccgt
     3061 aacaggcagg cttcataaat cgccgcctgg ggagtttttc cttctcccag gcgttcactc
     3121 tgcaaggcga aatcgatcat catgatggca tttttcttca caatgccgat caagagcata
     3181 attccaatca aggcaatcat gctcaactca gacttgaaca gcagcaatgc caacagggcc
     3241 ccgaccccgg cggacggcag cgtggaaaga atggtgaggg gatgaatata actctcatac
     3301 agaatcccca gcacgatata caccgtgact aacgccgcca ggatcaacca gggttggttc
     3361 tctacagacg actgaaacgc tttggccgtt ccttggaacg tccctcgcac actcgccggc
     3421 aatccgatct catgcatcgc ctggtcgatc gccgccaccg cctcaccgag cgacacgccg
     3481 gccgccatgt tgaaggacac ggtcaccgca ggaaactgcc cctggtgatt gacggacaag
     3541 atggcattgg ccggttcgta ccgtgtcacc gcactcaatg gcacttgagc accattcggc
     3601 gaacggacgt aaatttcgtg gagtgcgtcg ggattctgcc aatactgcgg cgcgacctcc
     3661 atcacgacat ggtattgatt gagcggcgta tacataatcg aaacctgccg ctgaccgaac
     3721 gcatcgtaga gggtgtcatc gatcaattga ggcgtcaaac cgagtcgtga cgcggtactc
     3781 cggtcgaaga tcaccagcga ttgttgaccg cgatcctgct gatcgctatt cacgtccaca
     3841 atctccggca aagtgcgtaa cttgttctcg actttaggcg cccaggtgtt gagttcctcc
     3901 agatccacgc tctggagagt atattgatac tgcgcactgc tggcccgccc gccgattcgc
     3961 agatcctgga tagcttgcag ataggtcggg gcacccggca cttttgccag cttcgggcgt
     4021 aaccgggcaa tgacttcctc ggaactcatg ccgcgctctt ccagcggttt caatgcgatg
     4081 aacatacggc ccgtattggt ggtgccggaa tggctccctc cgccggtgaa cccggtgatg
     4141 ctcgccaccg ccgggtcgct cttgatgatc cccaccacct cggtcaactt gtctcgcatg
     4201 gcctggaacg aaatatcctg cgcggcacgg atgtttccga agaggcgccc cgtgtcctgc
     4261 tgcgggaaaa accccttcgg caccaataca tacagataga tcgtaagggc catggtcgcc
     4321 agggtcacga tcaacatggt gcggggatgc cggagaaccc agcccagact cgtggcatat
     4381 cctcgcagca tcacattgaa tccccgctcg ctgatgcgat accaccatcc atgggcgaca
     4441 ttggtctccg acttcaatac tcgcgcacac agcatgggaa tcgtcgtcaa cgacaccagc
     4501 aatgacactc cgatcgccac cgaaagcgtc acggcaaatt cgcgaaacaa ccgccccagc
     4561 atcccgccca tgagaaacac cggaatgaac accgccacca aggatacggt catcgacacc
     4621 accgtaaacg cgatgtcttt cgctcctcgc aacgccgctt ccatggccgg cacgccctgc
     4681 tcacgatagc gtgtgatgtt ttccagcacc acgatggcat cgtccaccac aaaaccggtc
     4741 gcgatcgtca aggccatgag cgacaggttg tctaagctgt aatcgaggag atacatcacg
     4801 ccgaacgtgg aaatcagcga cacgggcaca gccaccgcgg ggatgagtgt ggcgcgaaga
     4861 ttccggagaa acacgaacac gacgagaatg accagggcga ccgagatcgc gagggtccgc
     4921 tccacgtcgt gcaaggaggc acggatgacg ggagttcgat ccatgacgat cgacagcgac
     4981 atgctccccg gcagcgacgc ttccagctgc ggcaaccgcg cgcgaagccg atccaccgtc
     5041 tcgatgatgt tggcatcagg ctgccggtag acgatcacca agaccgccgg tgtaccattc
     5101 gcgacaccca tagtccgcag gtcttccacc gatgattcca cggaggccac atccgacaga
     5161 tgcaccgccc gtccgtttcg ataggctacg atcaacggca gatattcatc cgctttatgc
     5221 aactgatcat tcgtccggat ctgccaggtg cgatcatccg aggtgagctg tcccttcgga
     5281 cgattgacgt tcgtactggt cagcacctcc cggacgtcgc ccagtccgat gccatactta
     5341 ttcagggcgg taggattcaa atcgacacgg actgccggca aggagcctcc gcccaccgcc
     5401 acctgcccga cgccttccac ctgcgagagt ttctgctgca agatgctcga cgccgcgtcg
     5461 tacatttgtc cgcgcgtgac gagttccgag gtaatggcca gaatgagaat gggcccgtcg
     5521 gccggattca caatgctgta gcgaggtgca ctcggcaggt ttggcgggag atcgacacgg
     5581 gaggcattga tcgccgcttg cacgtcgcgc gcagcgccat cgatgtcacg gtcgagctca
     5641 aactgcagtg tgacgttggt gcctcccatc gtgctggagg atgtcatttc tgtcactccg
     5701 gcaatccggg tgaactgccg ctccagcgga gccgccacgg acgaggccat gatctcaggg
     5761 ctggctcccg gcaacgaggc tgatacattg atcgtcggaa actcgacttg cggtagtgcg
     5821 gcaaccggaa ggaattgaaa cgccacgatc ccgatgaacg tcgcaccaat ggtcaataac
     5881 gtggtggcaa ccgggcgccg gacaaagggg gcggagatgt tcatggtgga cctatcggca
     5941 gcgcaccggc cacggacacc ggttcgcgac tgcggcggaa acgcgtcgca acccgatcca
     6001 aagccagata caccacaggc gtggtgtaga gcgtgaggag ctggctcaag accaatcctc
     6061 ccacgatcgc gatcccgagc gggcgtcgta attccgagcc cacccctgcc cccatcgcca
     6121 gaggcaaggc gccgaagagc gcagccatgg tggtcatcat gatcgggcga aaccgcaaca
     6181 gtccggcttc gtagatggcc tgctctggtg gtttaccttc gttgcgctca gcctccaagg
     6241 caaagtctat catcatgatg gcgttcttct tcacgatgcc gatgagcaac acaatcccga
     6301 tcagcgcgat gatgctgaat tccatacgga acaacatgag ggccagcagc gcgccgacac
     6361 ccgccgaggg aagggtggag agaatcgtca gcggatggat gtagctctca tacaaaatac
     6421 caaggacgat gtagaccgtg atcaaggcgg ccagaatcaa cagcggttca tgttccagag
     6481 agttttgaaa ggcctgcgcc gcgccctgaa aacttccgcg gatactggcg ggaagcccca
     6541 attcctgcgt gacatgccga atcgcttcca ccgccgcgcc aagcgaacca ttcggcgcaa
     6601 gattgaacga gatcgtcacg gcgggaaact gcccctgccg gctgatgacc agcggcacgg
     6661 tggtctcggt caccctggtg aagacattca acggcaccga tccgccgctg cctctcacct
     6721 ccagagcttg cagggcttcc ggtcctttct gaaattccgg catcacctcc agaatgacgc
     6781 gatactgatt caactgcgta aacatcgtcg aaacctgccg ctggccgaac gcatcgtaca
     6841 aggtatccgt gatgagttgc ggcgtgatgc ccatgcgtga cgcggtattg cggtcgatct
     6901 ccacgagcga ggccaatccc tgattcagtt gatcgctgct gacatcgcgc agttcagggc
     6961 gcagctgcaa atgttccagc agtttcgggg cccaccggtt caactcctcc ggatcggcat
     7021 cttccaacgt gtattgaaac tgcgtccgac tcacccgatc ttccaccgtg aggtcttgca
     7081 ccggctgcat gtgcaaggtg atcccttcca cgtcggccac acgtgattcc agccgtctga
     7141 tgatgtcacc ggcgcccccc acccgctcgc ccaaaggttt cagattaatg tacacgcgcc
     7201 cgctgttgag cgtcgtattc gtgccgtcca caccgataaa tgacgtgagg ctttccacag
     7261 ccggttcctc aagaatgacg cgagccagag cttgctggcg ttccgccatc gtgccgaacg
     7321 agaccgcttg cgggccttcc gtgatgccga ggatgacgcc tgtatcctgg agcggaaaaa
     7381 atcctttggg aatcagaaca aacagcgcga ccgtgaccac cagcgtggcg cccgtcacaa
     7441 gcagcgtcgc ctgttggcgc cgaagtaccc actgcagact tctcccatag cccgcgatga
     7501 tgcgatccag catttgctgc gaggctcgcg ccaacggccc cggatgctcc tcccgccgtt
     7561 cgcgcaaaag cctggcgcac atcatcggag tcaacgtgag cgacacgatt gccgaaatga
     7621 ggatcgtaat gctcagcgtc acggcaaact cccggaataa ccgtcccacc acatcgccca
     7681 tgaacaacaa cgggatcaat actgcgatca gggacacggt caacgacagg atcgtgaagg
     7741 cgatttgctt ggaccccttg agagcagcct ggaaaggcga ttccccctgt tcgatgtatc
     7801 gcgcgatgtt ctcgatcatc acgatggcat cgtcgaccac gaagcccgtg gagatcgtca
     7861 gcgccatcaa ggtcagatta ttcaggctga accccatgag gtacatgagg ccgaaggtgc
     7921 cgataagaga gaggggtacg gccaccgccg gaatcaccgt ggccggcaat gtgcggagaa
     7981 acaaaaaaat caccagaatc acaagggcca ccgctagcac caattcgaac tgtacatcgc
     8041 gaacggtcgc gcgaatcgaa gtcgtgcgat cggtcagcac ggagacctgc acggatgacg
     8101 ggagggttcc ctgtagctgc ggcagcaact gcttgacgcg gtcgaccact tcgatgacat
     8161 tcgtccccgg ctggcgctga atgttgacga tcaccgccgg cgtctcgttc atccaggcgg
     8221 cctgcttggt attttcgaca tcgtcgatga cctcggcgac ttccgacagc cgaaccggcg
     8281 cgccgttgcg ataggccacg atcagcgcct tatagtcttt gccggcaaat aattggtccg
     8341 tggcgttgat gatggaggcg cgacgcggcc cgtcaaagcc gcccttggcc tggttcacat
     8401 tggcggcagc caccaccacg cggagatctt ccaacgtcaa cccgtaggcg gccaacgcgc
     8461 gcggattcgc ctgtatccgc accgccggac gctggccccc gctgatgctt acgagaccga
     8521 caccggacaa ttgcgaaatc ttttgcgcaa accgggtttc ggcaaagtca cgcacttgcg
     8581 acagaggcaa ggtagcggag gtcaacgcca gcgtgaggat cggcgcatct gcggggttaa
     8641 ctttgttgta gaccggcggc gcaggaagat cgcgaggcag gaaggtggcc gcggaattca
     8701 tggccgcctg cacttcctgc tcagcgatat ctagatcgat gtcgagactg aattgaaggg
     8761 taacgaccga actgcccccg gaactcgtgg aggtcatctg attgagcccg ggcatttgcc
     8821 cgaactgccg ttccagtgga gccgtaatgg aagacgtcat cacgtccggc ccggcgccgg
     8881 gataaaacgt caacacttga atggtgggat aatcgacctg cggaagcgcg gaaacgggca
     8941 gatggcgata tgccagcgcg ccggccagca gaatcgcgat catcgtcaag accgtcgcca
     9001 ccggccgcac gatgaacagc cgcgaagggt tcacgtgcgt ttcccctgtt ttttccgttc
     9061 tcctcccgcg ggaggactct cctggctggt cggcactgct ccgcccgatg gacgcatctc
     9121 gaccttgctg ccttcacgca acttttccgt tccatccacc acaacggttt cacctgatgc
     9181 caggccggta tcgatcgagg tgtcttcgcc ctgtgacaca cccacggtca ctgcgcgcac
     9241 gctgaccgtc tgttccgcct cattcaccac gtagacaaat gttcccttgg gaccgcgttg
     9301 aatcgcggcc gacggcacga tggtcgcacc ttgcttgata tcaaggagca ggcgggcgtt
     9361 cacgaactga ttggggaaga gcgcgctgtc gtcgttggga aacaccgcct tcagacggac
     9421 ggtcccggtg gtcgggtcga tttgattgtc gacggtgagc aacgtccccg ttgccaattt
     9481 gcgcttctgc tcccgatcga aggcctccac caccagctgc tttcccgact tgagccgttc
     9541 gaacaccgcc ggcagatgat cttccgccaa cgcaaagacg acggtgatcg gctgcaattg
     9601 cgtgatcaca agcaatccgt tgggatcgtt ggcatgcacg atattgcccg catctacgag
     9661 gcgcagcccg acccgcccgt cgatcggcgc ggtaatgctc gaataggtca gttgcaactt
     9721 ggcattgtcg atctgtcctt ggtccgcctt gacgatacct tcatactgac gcacaagggc
     9781 ttcctgggta tcgagctgct gtttgggaat gaagttctgt ttgtacaggt cccggtagcg
     9841 ttcaaagtcg agctgtgcat tcttcatctg cgcctggtcg cgcgccattt gtccctgcgc
     9901 ctggatcaac tgcacttcaa aggggcgggg gtcgatctgc gccaataact ctccgttccg
     9961 cacgagttgg ccttccttga agagcacgcg catcaactgg ccatccaccc ggcttttcac
    10021 cgtgacggta ttcatcggga tgacggaccc caggccgttc aaataaatgt tgatctcacc
    10081 ggtttttgcc acggccgcca cgaccggggt attgcgcgca gccgtcggtg gacgcttttc
    10141 cgaccgactg ggttgcgcct caccggagcg ggtcatcgtg acataggttc ccacggccaa
    10201 taggcaggct gcggagagcc cgatcatcca tcgtttcaag agagtggaaa atctcaccga
    10261 ctcagccatc accgctcttc cttccgtccc ccggcatcct gcgcctgccg gacatactcg
    10321 cgggtcatgc gccagcgctg ctgcaactgg gcatggagtc cgtctaactt ggcctgctgc
    10381 tcaggcgata atgtcgcctt caattcctgc atcccctgat caagcactcg atcgacgtcc
    10441 ggttgatgct gcacgcgcaa ccgtaacaat tccacatggg ctcgcgccac gattggctca
    10501 accgcggctt gctgagtcgt cgacaatacc agttcctggg tcagccgttt cataatccgt
    10561 tcctgccggg ctcccggccc ccgctcccat cgatgctgat cttcgtactg ataatacagt
    10621 gacgtcccca cggcgcccat caaggctccg gcgcannnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnngatcg atacattcgg catcatcgga caagagtgcc tgcgctcctg
    10801 taaagctgac cgtcctccct gcttacagca tggacctcag catcataccc catccgccct
    10861 caggaccgca ggcgtggccc tcttattatg ttagtcgttg atagaccggc aaaggttaca
    10921 cgtgaccaca cacaatcgct caggccggaa ggctgactgg gccgtgccct atcaaccctg
    10981 gtctacgagt cacttacccg gcttctgttt ccagcgcttg ttaacgacga ggcgtacctg
    11041 atcagccaga gcccgggccg ccccgcttcg gtacccgccc ttcttccata acagcccgag
    11101 agcgcggcga ggtgtgggat tgttgagtgg aatcccgcga agcccgtcat gccgctctaa
    11161 gcccagagac agccggggaa gcacggtcgc cagtttgctg gtcttcaccg tcgcgagaat
    11221 cccttcgatc gaattcattt ccacagtcac cctaggcacg atacccgcct gttcaaaact
    11281 tgcattcagt aaacggcgcg tacaaaaaat acccggcagg agcaccaggg gctcatccgc
    11341 gagagacgcc aatggcaggt ggcggcgtgt ggcaaagcgg tgccggggtg acgcaatcaa
    11401 gacaaaatct tcctcaaaga gcggctgagt tgctatccga tccgatccgg gaggaacgaa
    11461 cccgatgcct aattccagga ctccgttctt cacaccggct tcgatgtccg gcccggatag
    11521 ttcatcgaat ttcagcgaga cgtgcggatg cagcgtcgag aaacgtccga cgatttcggg
    11581 aaccagatag gcattgacgg tttgcaccac gccgacggtc agggtaccgc gctgcagccc
    11641 ttcctcttca gcaatggcca cttgcgccag ttccatttca cgcagggcac gccgggcatg
    11701 ttcccgaaaa atcgatccgg cccgggtcaa ttggaccgat cgcccaaccc ggtcgaagag
    11761 cggcacgccg acttcctgct ccaactgttt gatctgggcg gacaaggccg gctgagagac
    11821 cgggagccct tcggccgctt tggtaaaatg caacgcgtcg gccaccgcga gaaagtaccg
    11881 gagatgacgg aattccatac cacctcttat cataactaga agtgattgat ctaatcagaa
    11941 caaacacttt gacgcctgaa cgggcctggc actagggtga cgccggtcac tcgcgtcgtt
    12001 gtctaacgga tcgaacagca tcacatgcag caggtcagcg gtttcatacg ttccattcag
    12061 gtgggtctgc ctgagcaggg tcatccgcac tgccacgaag atcgcccccc gcaatcctgg
    12121 tccaccggca tcttcaagcg tccagtccgt ggccccattc gcctccagca aaagaacttg
    12181 gagggtgacg gccaagcaga tctcatccac cacggaggcg tcgacaaggc ggtctgtgtg
    12241 tatccgtccg agcattggtt tcactggagc agcgtcctcc cgcagcaaca actcatcggc
    12301 ggcgagttcg gcgaaaactt tactctcgaa ggactgacgg aagcagacgt gtgcattgga
    12361 gacatttttt cagtggggtc cgctgtcgtg cagatatctc aaccacggca gccctgctgg
    12421 aagctggccc ggcgctggca gatcaaggat ctggcggtgc aagtggaaca gaccggattc
    12481 acgggatggt attttcgcgt ccttcaagaa ggtgtcgtcg aatcacgcat cccgttacac
    12541 ttaacggatc gcccgtatcc cgaatggacg gtcaccaccg cgaatcggat catgcatcag
    12601 gaacacagca acctgaaagc cgctgaacac ttgagcctct gcccgttgct ctctgcaagt
    12661 tggcgacacg cattgcacca gcggagccag cagcagaaca gtccggatcc gcagccacgt
    12721 caattcggca attctcaatc ctaatcacca ttctttccgg aggtgttatg actcgtctca
    12781 ctctatccgt ctgcagtctg gcccttggcg ccattctcgc catgagttcc tggtgcgccg
    12841 ctgaaaatgt gaccaccatc aacattttgt ctccgcgcaa cggcgactcc gtcccggaaa
    12901 cgttcgaaat caagtatgag attttgaatg cgccggaggg gaatcatgct cacgtctacc
    12961 tggatggcgc ctaccagaag gggttcaagg gtaccttcac gcacgtgcct aagggcgaac
    13021 ataccattac ggtcaaaatt gccgatcatg atcacaaaga tgcaggaacg gcgacagaca
    13081 gcgtaacggt gaaggtcggg gagtaggagg aagcctcggg cgacgagagt gcgcctgata
    13141 cggtgcggct ttcaaaatcg cacggccgga aggcggccca ctgtccgccg ccttcctcac
    13201 cagtgctcag cgcctattgc ccgccctgtg tcgacgcacg ggtggacgag ctctgcagat
    13261 ccatctcaaa cacgaccgga ctgtctggat ttttcgggtc gatccggagg ggcatcatat
    13321 cggccgcgag tttcaccagc ggcaccgcca caatgtgcgg agccttttcc gatggattcg
    13381 aatggagtgc gatacgaacg ctcttcggcc gctgactagg cgccacttgc ataagcacgt
    13441 ccttacccat aaacccaatc agcaagccgc ttccatcagc cagcttatgc acctcatacg
    13501 cactcgttgc aggagacacc cgctccagaa cgagatccgg actctgcgtg accagggcca
    13561 gccccaggaa ttttggcaga ataaagccaa acgtgcccgt cggggcttgg gaaaacatcg
    13621 tcttggaggt cgacgcgccc agttccgcca ctccgttctt gctgcgtaac tggcgacgca
    13681 gatctgcttc gcttcccgac gcatcaatga tcacaaccga aggagccgct ttttgaaccg
    13741 gcaagatctc cgtttttcct ccaccgggac tggtcccgcc gcgtgcagca ttgagggggg
    13801 ccggcagaag cgttgtgaaa agaacaccca ccaagcccgc ggccgcgaga tgacggagcg
    13861 gcgcctggaa catctttcga ttgatacatt cacacatgag tactgatcct cctgaacaag
    13921 ggagagctgt cgttccggag gattctaaga agccgactca cgaaaatcaa acaggaagga
    13981 gtgcacttgc ctcacggata gaagctgata cggcgtccgg ctatggcctg ggcgacggat
    14041 gccaggatct gctcccgctc cgccggtttt tcgagaaatt ccacaatgcc ctgtcggcgt
    14101 aacgaggagg ccaatggctc gtcatgatag ccggtcagta cgatgaccgg cagagatgga
    14161 tattgggctc gaaaataggc aatagcctcc acaccattga tgcgcggcat ccggatatcg
    14221 caaataatcg catccagcat cagcgggttg tcgccgctgt tcaacacttc caccgctttc
    14281 ccgccgtcgt ccgcctcctc cacatcgtac ccggcctgcg tcagcatcat gcgcagcacg
    14341 ctgcggatcg agtcttcgtc atccaccacc aagacccggc ccaggctgac cttgtcgccc
    14401 gtacttagtc cgaacggcca tgccattccc atggtgtccc tcccttcgcc agacgcatgt
    14461 gtttccctgg gttggcgatg caaggacaag gccttcgacc tatcggactc gacatgaccc
    14521 gattcaacaa gttagcgatc gagatcttct gtccatcgcg tggaacgcct ctttcaagtg
    14581 gtggaatcac accacctcgg tgcaaatgca ccaaagctca cagtgaagta tggtgagggg
    14641 tggaagacgt tcaggaagga cagcggccgg cacagagatg caccggccgc gtaaacagct
    14701 ggggcggcct cgacatcaac atgtcctcat agcgatacgg tccgctatag agaggaggga
    14761 ctcacgccgt cagtttgcgt gcaaccacca cacccgagac cacaaaggtg aggacgatcg
    14821 ccagaaagat gtaaaacaga atttccgcta catccgacgc cttgtccgca attcctgtga
    14881 atccaaatgc cgcagccacc atggccagaa cgaaaaatac tgctgcccat ttgagaaaca
    14941 tacccacctc ctcggctcag attcccgatc ccagcactcg actttaccga atggcacgtt
    15001 cgctcaagat ctctgtgtac aattggcagt accggtcggc ctgctcttcg agagaaaaat
    15061 ggttgagggc atattggcga cagtttgcac ccagcgccaa gcggcgctca gggttctgca
    15121 acagttccag catggcagca cccaatgctc ctgcctcacc aggcggcacc agcaagccgg
    15181 tctcacaatg ctgaatcatg tcgggaatcc ctccgacttc aaaccccacc gccggcgttc
    15241 cacaggccat cgactcgagg accgtattcg gcaagttatc ctgcaacgac gggatcacga
    15301 gcagatcggc tgcgctgtaa atgttggaga gcaatcgatc gctgttgatg tgtcccaggt
    15361 aggagtgagg aatatgatgt cctaggtcgg ggctattatg gcccaccgtg acgagatgaa
    15421 ggtccgggat ccagctgcga atatgctcca aggcccgtac cagaacctca aatcccttcc
    15481 ggaacagcgg gagcccgtcg gccacgaaca gaatgacttt cgctttagga gggatcccga
    15541 agacctctct ggcactgccg acatcgcggg gcgagaactc cgtgaggtcg agcccatagg
    15601 gaatcaccga cacagggaac cggccgagaa tcgggctgcg ctgcacttcc tcggcgagcc
    15661 acgtgctcgg agtcgcaaag tgaatctgcc cgtcggaaag ttggtcaaag agtgatttct
    15721 tgcgggccca gatttcatgg gagagatcat tcggccctgc ggcagccagc tgaggacagt
    15781 ctccgcattc accgagatag cggccgcacc ccagatcgta atgacagccg cccgtaaacg
    15841 cattcatgtc gtgaagtgtc cacaccacgc gcgtattctt cgagaaggcc ccaaagaagg
    15901 aggggtaatc cacaaattcc gcaatccagt gcaggttcag aatgtcatag ggcgggagtt
    15961 tcgacaaaat gtcggtccca tattccgagc gatccgttcg gaatggttca atcttgtcat
    16021 tctcgggaga gatggcgggg cggagcttgc gttcgatttg ttcacgccgg atgcggcgct
    16081 cggctcgttt acagaaagtg gtggtcggct cgaaccgaat tatagatccg tcagaggttc
    16141 gggcttcccg gacgaacatg gacgaatcga cgcctgccct gcgcaatcca tcatgcagcc
    16201 gataggccgc ccgcgcggca ccgccggcga cgtcgtaggt actgagatgg acgactttag
    16261 gaggacggat gcgagcgctg gggcaaagat cgatgttgtc ttgttcgtga ctctctattt
    16321 caaggggagt ggtgcttctg gacatgattg ggttccctat tcatcttcca ccgtggtgcg
    16381 cgggaaaatg tcttgagatg agtcccgatc atgcctgctg aactctcctt gctccactag
    16441 gcataccccg aggaatcact cgtcgcgagg ccgctcgaaa tggagtgatc acaggaagga
    16501 gtgcaccgcc tcgcgggccg tctgcgtcca atcatatttc accaacacag acttgcctct
    16561 ccaattggga ggataacttt ttccagtccc gtaaaagtct ccgtcgacaa cggacaatag
    16621 gctggcgtta tgcacccaat cctgaacctc tttgtcactt tccacgtacg acatcacata
    16681 gtactggccg ccggagaggg gccattccgg aacccggaaa tcaatgtagc cgtctccgga
    16741 caaatcgagt ggtagcgggc tcacgagatc ggtggacatg agaaaatacg ccgtctcata
    16801 ggggtcgccg attcgagcat gtaacgaaag ttccacgcgg catcgccgaa tgattttgtc
    16861 aggcacgcac cggtaatgcc atcgcacgat ggtctctcgc ccgcagatgg ccgacgacga
    16921 gacgttccga tactggtcga gcacctcgat ggcggtcgca atgacgtggc ctttcccttg
    16981 tctgtccgtg cgtgtgtcca acgcacttct ggctaaatcg gccgtcatgc ctgcataggc
    17041 ctgcaccaca tccctggctg taccggtaag agccacacgt ccctgtgcga ggaacagcgc
    17101 acgctggcag agattttcga tgatcggcaa attgtggctg accaccaaca ccgtccgacc
    17161 gccccgactg gcctgccgca tcttccctaa acacttctgt tgaaaacccg catcgcctac
    17221 ggccaggact tcgtccacga taagaatgtc tggctcaaaa tgggccgcga cagaaaaggc
    17281 caagcgcaca tacatcccgc tcgaatagcg tttcaccggc gtgtcgataa actcctcgac
    17341 gcccgcaaaa tcgatgatct catccaactt ggcggtgatc tcggcccggc gcatcccgag
    17401 aatagaccca ttcagataaa tgttctcgcg ccccgtcagc tcagggtgaa agccagtgcc
    17461 gacctcaagg agcgaaccga cgcgaccata gatatcggct gtccctgacg tgggcgccgt
    17521 gattctggac aggattttga ggagggtact tttcccggcc ccgttatggc cgataatgcc
    17581 gaacacctcc cccggcttca catcaaagga gacatcagtg agagcgcaaa actctcttcc
    17641 cttcccggta tcatcggaac gacactgctt ccaccaggag agcgcagccg cgaggcgatc
    17701 acgaagcgta tcgtgacgcg cctgcaggcc gagggaatag cgttttgtca ggcgatcgac
    17761 tgagatggcg acgttgccca taagtccgca tgtagtggat atacgatatt tcgctccact
    17821 tatgtgtcac acattcgtct tcagatcacc tcggactttc cctagcagat gggtggatgg
    17881 attaagcgtg agtcgcctca accagggatc tattttgttt ccaccgccgt tccatacact
    17941 tgggtgcacg cacgagaaaa cagaatgaag tattgaaaat agccgtccac cgtgacctca
    18001 accattgcgt cagccaaggg tttcgtgcgt aacgcatcct cgacggcatt gcgcatctca
    18061 ttcccgccgg tcacggggat caatccgaga agatggtaga cacaatcctg cccacgcacc
    18121 ttgcccagtt cggtatatcc tcccggagcg agcggcttgg ttgaaggcgc gatgcctccc
    18181 ggatgatgca tgcatcccgc ggtgagggct cccaccgcca acatgacgac catccaccca
    18241 cgcctagacc agttgctcaa acgttgactc atcatgaggt tctactcctt tcatgcgcat
    18301 agccttcacg acatgcagtg tgacatccac gtcggacata ccgttgtgag gatgtatgag
    18361 gaagttggta ctgcaaggaa ccggcggagt acggcgagca cgtttcccca tcttgcacag
    18421 atagcccttc agcctcaacc ctaaattgcg acggcctagc acatgatcga atgtgcgaca
    18481 tcctctcgcg ggctgcttgg cagggcacgt cgccgccgga actccaaatg gaggtattac
    18541 ggaacagcag aaccttaccc tcgccaccga gcacatcatg ggcaatttca ttgcctgtca
    18601 gggaatacct gatagtattt attcacggaa catgagacct tcgccggctg ccggatgatg
    18661 gcggtgtgag ccgatcacac cagagaatgt tccggcgtgg gagcgcacgc ctctcgcaga
    18721 gtgcggctgt ataccttggg cagaaaactc gtcatgatca ctgcgtcggg cgcggtgatc
    18781 ctcatactca tcggcctgct cctggggttg ggtgaggtga tcattcgttc cgttcacctc
    18841 attcgagacg gcattccttt ttttgaaagc aacgcaggcg ggcgaatcgg tcccattacc
    18901 ctggatcagg acctgggctg gaaggccact gagcattatc aggaagcctt ggtggaaaag
    18961 accaagaacg gcaccctgta cccggtccgc cgctctcaaa cgcaatatgg ctttcgcagt
    19021 ttcggcgatg tgtcgtcccg ccgccccaaa atgttggtga tcggcgattc attcacccat
    19081 gccaccgcgg tctcagacga ccggacctac cacgctttat tatccaaact gctcgatgtc
    19141 gaggtctttg cctatggggc aggaggctat ggcacgttgc aggaatatct cgtgttcgac
    19201 cggtacctcg atatcgttcg cccggatgtc atcctgtggc aatactgcat caacgacttc
    19261 atcaataacg acaatgagct cgagcgcctc agccgcgtca acaacaatgg ctgggtgcga
    19321 ccctactggc gcaacggcac catccaactc ctctctccga aggaatcatc gcttcagata
    19381 cgagactgga tcaatcgcca cagccgattt ctctatttcg tcgtcaccag gctggatcgt
    19441 ctgcgagccg caaatacgcg ggatacggtt gaagtggaca tcgaagcgga aggcatgcga
    19501 catgccgggt tcgctcgatc ggtcgctgtc acggatgact tgatgggacg agttcgggca
    19561 cgggcgggta cgcggccgat catggcattt aattgcacgg aggccgaacc ttacagccag
    19621 gcttttcgcg atatttccgg gcaccatgcc attgcctatt ggggcgatgt ggctcaggtg
    19681 gttcaagacg cggacacacg cggggaggat gtatttgccg ccgatggaag ccactggaac
    19741 gagcgggggc acgaactggc agcccaggcg ctggcgaaac atttcaagac agcactcccg
    19801 cccgtaccgc gcccctgacg tcgcctcacc tatcgcaggc ggacgatgtc tttcatccaa
    19861 cgtaccccac cattgcgact attcccgtcg cttggagccc ctggtgcgag acgctgactt
    19921 ccgtcgagac gctgaggtgg cccgtttcga accggctgca ggcaccttca caccggcttt
    19981 tctcgccttc gaaaggccga tcgcaatggc ctgctttcta gattgcacgc ggtgtttgcc
    20041 gcgcttcatg tgttccattt cttcttctac gaatgccccc gcttgagtcg taggcgcctt
    20101 cccttcccgt tttgcctgcc gcgcccgttg catggtcttt tgttcaggca tacagtcccc
    20161 cttggcgtgc tgcttgttcg cgcgcgcttc tggtcgttcc gccggtcggc gaaaaaggct
    20221 caccgtccca acgccgatca ttccgtcgag tggagaggcc cttcgcgaga taccctccga
    20281 ccgccaatgc cgcagctgcc acccacagac gccgctgatt gagccacaat tgcaaactga
    20341 actcgtaagc gccttcgtcg aagccgccat gtgcgccgtg gtcacctgga agtgggtgga
    20401 agaggttgtt gggtcgatcc ggagctatag gctcgttcgt ttgctgcgcc ttatagccct
    20461 tccaggccaa ataatagtcc aacagcccgg gaataaactt ctgcccgacg atggctttca
    20521 ccgtcggcag cccgatcgag atctcccggc gacgatggcg cgaagcccag acgatggccc
    20581 tggcgatcac ttccggctgg taaatcggag ggaccggttg gggatggcaa ggcatgcgag
    20641 acttgatcca gtcgaattga ggggtattca ccgccggcaa ctgcacagtc gtaatatgga
    20701 cgcggcttcc atcgtaaagc agttcggacc tgagggaatc gacgaatccc tgcaccgcgt
    20761 gcttcgcccc acaataggcg gactggagcg gaatcgatcg ataggctaag gccgagccaa
    20821 cctgcaccat cacgccgaca tctctcggca ccatgcgtcg caacgcagcc aatgtgccgt
    20881 acacatagcc gagataggtg acatccgtga cgcgttgata ctcctcggcc gtcatcttca
    20941 gagccggcga tagaaccgac gccatggcgt tgttcaccca gacatcgatc ggtccgaatc
    21001 ggcgctcgac catcgccgca gccgcttcaa cctggtcggg atacgcaaca tccgtcggaa
    21061 gcaccaacgc ccgccctcca gccgcctcga cttccttgcg ggccccttcg agtccatcgc
    21121 ggctcctggc gaccaatcca atccaggcgc cctccctggc aaactcccgc actgtcgccc
    21181 ggccgactcc cgcggaagct ccggtgatca caacaacccg tgggtgatga tgacggtgag
    21241 ctttcataag tccttcgatc gtgagtgaac agacgccttc gtgatgatct gagagcgtac
    21301 gcccagagca tcggattgga actggacgaa accctggatg gttgctcgct cagcgcatgg
    21361 aaatttcagc taatggtccg acacagatcg tgagtccggc gtgacagggg cggcaaacat
    21421 gcggtgtatc tgaatctgat acaacaccac aaccagcaag ccgaacggca gccaccacaa
    21481 ggcggcctgc aggccatctt tgcctggaga ggcgttgtga atcgtcacac tgtttgccgg
    21541 ttcggtcgtc gcgaccagga gattcgggta ggtgccccat gcaatcacgg cgatgaacac
    21601 agccaacaac acgctcgacg aagcgaaagc agccacctgc cgttggcgcg tgtgactgag
    21661 ggcgagggag acgaacgcga gaaggccaag cacgacccac aggtacccgg caggatgagt
    21721 gtggaaattt accgtgaagg tagcccgcac aaacgggata gcgacgacga agaccaatac
    21781 caacaggaca gcgggcaaga gggcccagct cgctagcgcc ctgctcctcg cctgcagccg
    21841 gccaacagtc ttcatggcta gatacgaggc gccgtgcagc gtcagcaggg ccaccatcgt
    21901 caagccggcc aggagggtaa accaatcgag aatgccgggg ttcaacccgg ggcgaaggtc
    21961 ggtccagagc ggcaggaaaa aatacccgtc tgcatccagt ggcacaccgc ggatgacgtt
    22021 accggcgaac agtccgagaa gaaacgccaa cgacaggctc gtcacgaaaa agatcgtatc
    22081 ccaaaagcgc cgccagagtg gatgttcgac atggcctctc aattcaaatg ccagtccgcg
    22141 ccccatcaag agccagagca ccatgatgaa gcctaagtag aatccgctga acccggctgc
    22201 ataggctttg ggaaaagaca gaaacaacaa cccgcttcca gcgatcaacc atacttcatt
    22261 cccggtccat acagggccga tggtggcgcg caccatacgt cgctcggatt catctgtggc
    22321 cacgacggga aaaagcatcg ccacgcccaa atcgtatcca tcaaggacga tatacatcgc
    22381 cagcacgaag gtgagggcgc tgaaccacaa cgtttccatc acagttcccc tgactgtccg
    22441 gcagtcgccc catggtcaat cggtccccgt cgaagcatct cgagaaataa caatacaaac
    22501 agcagtccga gggcgagata gagaccgaga aaaccgagga gggtgaacag tgcgttaccc
    22561 gaatgcacga gaggcgagat gccctccgcc gtgcgaaaca ggccatagac cagccatggc
    22621 tggcggccca attccgccgt catccaaccg gcagtggtgg cgatgtaggg agcgggggcg
    22681 ctcaacaaca gcaaccagag cagccagctg gaggcaaaca atctgccgcg ccataaccac
    22741 aggaatgcca accccatcac gacgaccagc cacgtcccca gtccggccat gatgtgatag
    22801 gaaaaatata agagggcaat atggtccggc cagtcctgcg gaggaaaggc gtccagccct
    22861 ttcacgttgg catagagatc gtaatacacg agcagactga gtgccgacgg gaccacgagc
    22921 gggttatcaa tcgtcatcgt ctcaaggttg ggctgaccga tcagaaggat gtctgccccg
    22981 cgctcgctgc gaaacaggcc ttcaaacgcc gctcctttga tgggctgatg ctcaaacacc
    23041 tgtcgcgcgc tttcatgacc ggtcggagta atcaaaagcg ccgaggccac cgcccccgcc
    23101 accactccgg tttgcacata ggtcttcgca tgcaccagat gccgaccagc caagagatac
    23161 agtgctccga ctgccgccat gacgaatgaa ccggttgcga ccgcagccgt catattgtgg
    23221 gtgaattgcc agagcaacca ggggttcgtg agcagttccg cgaggctctc aatggccaac
    23281 ttcccgttgt cgaggacacg atacgccacg gggtgctgca tccaggcatt cgtggccaga
    23341 atgaaatagc cggaaagcca ggaaccgacc cacaacatcg cgctcgccac ccacagaatc
    23401 cggcggctac atcgagactc accgaagagg agcactccca ggaacgagga ctccaggaaa
    23461 aaggcgaaga ctccctccat cgccaacgtc tgaccgataa tcccccctgc gtaattggaa
    23521 aacgccgccc aattcgtgcc gaactgaaat tccaacggga taccggtgac gacgccaaaa
    23581 gcgaaactca gggcaaagat gccggtccag aagcgagcga cctcatgaaa gtgctcaccg
    23641 acgctgaaga gatccctggt gcgaaagtac aggacgaaca gcgccagccc catggtgagt
    23701 tggggaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct ctgacgatgc
    23821 atcccacgaa cggctcgcac aaaccctcgg atggttcagc atcggactgg ggatggcaga
    23881 actctttgcc ccccgaaccg tcgctcgcat agccggcctg ccacacgatc atcgctggct
    23941 gctaggagcc tctggattac gagagattac gatggggctt ggcatcctct cggagcgacg
    24001 cccggcgggg tggatgtggt cccgtgtcgg cggagatgcc ctcgatctgg cgctcctcgc
    24061 tacagcgctc tccagtgcgc aaggaaaccg caagagaacg gccgcggcga tgctggccgt
    24121 cgcgggaatc gcggcggtgg attgttttgc gagccggcat ttgagcagga tggtgggctg
    24181 gacgagtgac gatggagccg tcttgatcaa aaaaacgatc atgatcgata gttcccccga
    24241 agcgctctat ctcttctggc gcgatttcca aaacctgtct cgcttcaccg agcacgtgga
    24301 atcggtgacc atctacgacc ggcaccattc tcattggacg gtgaaaggac cggccgggta
    24361 tcgggttgaa tggaacgcgg agatcaccga cgacgaaccg aaccgaaaga tcgcgtggcg
    24421 gacgacctcg caggcggaca tccaccacac cggctcggtg gaattcacac cgtcgcccgg
    24481 cggacggggg actcttgtgc gagtcgtgat ggaatacaac gtgccggctg gggtgctggg
    24541 tgcttccatt gcgaaactgt tcggcagcga acccggccag caggtccaga gtgatcttcg
    24601 ccgactcaag caaattattg aagtcggtga agtgctgaga tcggacggat cgttacatgg
    24661 caaagggatt ctccagcaac ggccggcgca accagccggg agtcacactt aaacctatcg
    24721 ttcgtctatt tgcgtgaacg acggaagcaa gggaggatcc ccatgaaggc gaattgttgg
    24781 tatggcgcgg gcgatgtccg cgtggaacag gttcccgatc cgacgatcct caatcgccgt
    24841 gacgccatcc tgaaggtgac ttcgacggcc atctgtggtt cggaccttca tctatacaat
    24901 ggattgatgc cgaccatgaa ggccggagat attctcgggc acgaattcat gggtgaggtg
    24961 gtcgaagtcg gcagcgaggt caccaacttg cgcgttgggg atcgggtcgt cacggcgttt
    25021 ccgattgcct gcgggcgatg tctcccctgc cggcaggaat tattttcgct ctgcgagaat
    25081 tccaacccca acgcgtggat ggcggaaaag ttgctggggc attctccgtc cggaatattc
    25141 ggctactcgc acatgctcgg cggattcgcc ggtgggcagg cggagtatgc ccgggtgccc
    25201 ttcgcggatg tggggacgat taaaattccc tcggacatcc ccgacgaaca ggtgttgttc
    25261 ctctccgata tttttcccac cggatacatg gccgccgaaa attgccagat ccaatccggt
    25321 gacacgatcg ccgtgtgggg ttgcgggccc gttggacaat ttgccatccg tagcgcgttt
    25381 cttctgggtg ccgaacgtgt cattgccatc gatcggtttc ctgatcgcct gcagatggca
    25441 cgggatggag gcgccgacac catgaactat gaagaagaag aggtctatga tcggttgatg
    25501 gaaatgaccg gcggtcgagg ccccgacgcc tgtatcgacg ctgtgggcat ggaagcgcac
    25561 ggccccggcg tccttgccgc ctatgatcgg atgaaacagg ccgtgatgct ggaaagcgat
    25621 cgtccgtatg ccttacgaca agccatcatg agctgtaaaa acggcggcac cgtctcgatc
    25681 atcggcgtct acagcgggtt tatcgacaag tttcccatcg ggtccatcat gaatcgctca
    25741 ttgacgatca aaacgggaca ggcccacgtg cagcgctaca tggcgccctt gttggagaga
    25801 attcagcgcg gagagatcga tccgagtgta attattaccc atcggatgcg cctcgacgaa
    25861 gcgccgcagg cctttgcaaa attcaaccgc aaggaagacc actgcatgaa gatcgtgctg
    25921 acgccctagt cctgcggacg cgtcagtcta tttcgcagat tttttggcgg ggactttctt
    25981 gccctttttg cgagcctcgg acaacccgat ggcaatcgcc tgctttttgc ttttcacctt
    26041 tttcccgctg ccgcttttga gcgtcccctt cttacgcttg tgcatggctt cctggacggt
    26101 tttctgcgag cctttgctgt acctggccat tgtgcgcctc cttgtggcaa acgagaatat
    26161 tcctgccggt gaatcgtccg actaatggcc tcctcgtgat ggatcagaga catcatggat
    26221 cacttcaccc gacgtgtgct gatccaatcc atcgatagcc agatcgccta aggagacgat
    26281 cccgacgagt tgtttgtttt ttgacagaat cggtatccgg cggatctgtt tctccgccat
    26341 cagttccgcg gcgacctcgg catcctgatc ctcgaagcag tacactaacc ccggcgtcat
    26401 cgcgtcactc actaaggcat cgaccgggct tcgactcaag gccagaacac gcaaaacgag
    26461 atcgcgatcc gtcaccatcc ccaacagttt ttctccatcg cataccggga tagcacccac
    26521 atccaggtct cgcatcttgc gcgcggcgtc agcggccgtc gcattgggag cgatcacctc
    26581 gacactcggt gtcattattt ctttgagtaa gggcatggtc ctcctttgcc ggcctatctc
    26641 aatcctatgg cggcagcgga tgaggaatgc atgccccact cgctgccgcc tgctcaccgt
    26701 cgatacgaat acatctcagt tttgcccttc ggtggcccac ctctgggcgt taccgcccgc
    26761 cgccacctcc agaaccgctg ccacccgagc ttcccccgct tccggagccc attcctgtcc
    26821 cacctcccat gccggacccc gaccccatcc cggaccctga ggaagatgac cctccccctg
    26881 tgcttccttt actgccttga gttccccctg aacctgtctg gccctgccca gcctgagtct
    26941 gcccagcttg tccctggcct tggctgccag acccgccatg agtccccgct tttccttcag
    27001 tgacagccgg cggcgtgccg ccgcttgaat gttgtgcgcc ggatggcagg gcagcacaca
    27061 cgagaacacc tcccgtcaga caagcgatca ccaatgttgt gtggttcctc atacgatgct
    27121 cctctcaaag agaaggatta gagacgatcc ccccggtttg taccccgccg ccagacactc
    27181 agcttcgccc tgacttggga gtggcttttg atctcgaccc ggatttcttc gaggactttg
    27241 ccatcaacac ctctttgcgt ttcatgaccg ccacttcaag cgcatcccaa tcgatgtcgt
    27301 gacgttcagc ctccggcagc atctcgcctt cttcttcctc aatgtggtgc ttgacgagct
    27361 cgctcaacac actgaacttc gcatgaaact tttcatcctt cggctttaac ttcttcagtt
    27421 ctttgatgag cacgtgcacg aggtgatgct cttccacagc ttcattcatc atgtcgctgt
    27481 cgtcaatcgc ctcacggatc gcggggtaaa tcagcttctc ttcgagttcg gcatggatct
    27541 ccaattcaaa aatggccgtc gcggcgattt ctgcttgctc ctggccgtcg gcagattcaa
    27601 attcctcgaa caaccccttt actttggcgt ggtcctcttt cagcatctca acaacccccg
    27661 atgcaaatgc ggttttccct gccatgacgg cctccttatg ggttaaatcg gctcacactc
    27721 tcgcaacagc ctatcaaaca gattccatca ggagagctcg tgtcatccct agttcgtgaa
    27781 gggagccacg tcatgtggtc gtcacagaag aggacgatgc agggagcaaa gagaggactt
    27841 tatgggttcc taatacagcg cggaaagaca aaccagagca atgcgaacag caaagctgcg
    27901 gccagggcca ccatcagact gatcgcgtca cttcccgtga tgagcgcgca aaccacatag
    27961 acgtcgaggc agatacctac gctcagcagg atcatgctca acaacagcag cagagacgag
    28021 agctggacga aggtacgact gatcgcgaac tgatgggcct gccgatgata ggccgccggc
    28081 gtcatgacga aggcgatcga aatagtgacg aacacaatag cgagcaaatg caacgtctga
    28141 tgcgcctccg gcatctcgcc aaatttgtta ttgaacaccg cgatgagctg aaacccgaat
    28201 agcgcttgaa taccggggag gaccatgcgg cattcttcga ggagatgtga ggcgacctcg
    28261 gaagcggcct caggttgagg gagattcgga gcggaatggc ttttcaacat cgccatacaa
    28321 cctcacgcct tctagcacat caaccgatgg ctctcccggc aatccctcgt cgaggctcgc
    28381 attgggagtg catgtcggcg caaactctct tcaaacagat cgcgattgat cgcctgcccc
    28441 gcagtggccc cttgtccggc ggcgatgatc atctggcaat tcgccggtgt cagacaccct
    28501 gcggcataca agcccgggac actggtttgc atgcaatgat ccaccaccac ctgaccttct
    28561 tcattcaagg cagcaccggc ctgggcggca agccgattgt gtacgacatc gccgcgagtc
    28621 gtaaatacgg cgtcggcctc gaactgttca ctggactcca acgtgaacga cctgatgaat
    28681 ccgctttcat gatggacggt acggatgcgc gaagacgata tgctcacgcc atactcgttg
    28741 agccacgcgg catgggtcgt atccacgccg cgggccggcc gttcgtgcat actttcacgt
    28801 tcgatgaaaa cgtcaacatg cccaaggcat actctgccgc ctcattgttc catccgataa
    28861 tgacaattcg ttgaccttgc acccggtagg cgtcgcaatc cttacaaaaa aacaagctgt
    28921 gtcccaggca ttccttcacg ccgggaatat ccggcggcac atgcgttagg ccggtggcca
    28981 gaagaatccg cccggcttca taacgggttt ttccgccctc gagctggaac ggatcgccaa
    29041 cactgagcga gacgacggtg tcttcgatca cctctacgcc aaattgcatc gcctgtgtca
    29101 gccctcgctc gagcagatct gtaccggcaa taccttgcgg aaatccgaga tagttttgga
    29161 cattcacttc ccatttggcc atggaatgac cagagtgcag cagcaatgtg cgtcgccggc
    29221 ttcgaccgag gtatagcgcg gcagaaagac ctgctggccc acttccaaca atgatgacat
    29281 cccacccctt cgcgtcgcgg ttcatggcta tcctggttct agcccgcatt gtctggcaaa
    29341 gcgggatggt gcgcgcctct tcctcattga ggagtcccac acttggcgag cgcctccgcc
    29401 ggagctgtcg taatcagttg ctcggagagg caggtgttcg cgtcaaaata ggtacgcata
    29461 tgctccagcg cctgctcgtt cacctgagca ctcaacgatg ccgtacagtc agaaggcacg
    29521 aataagccat acccacgcat atacgcatcg gccgccgtat agagaatgca ctggtcgccg
    29581 gtgactccag tgaggatcag acgccgaacg ctgagcgatt gcagcaagat ctccaacgga
    29641 gtggcaaaaa acgcagaatg ctttggcttc aacacaaagt agtcgttctg cgatggagcc
    29701 aagagctcag caataccccg tcctctgcat ggttcatcgc gacactgccg gacgagggtc
    29761 tgaaaatcat ggcgccatgt cccgaaatta tcgttgatat agatcacggg cattgcagcc
    29821 tggttcgcct tctgcttgag gcggcagatg cgtaccgcca catcgtaggc ggccggaaag
    29881 agatcttccg cacggggaaa ggtgaacggg ttgatcatgt cgatgatgag aagggccatg
    29941 tctgaaagac cggacctgcc cccgattgac tcagcggatt gattcggaag ctccattgct
    30001 gcgaccaccc tcacaggaaa ttatgattcg tagaacggtt gccccataaa ccgtgctcat
    30061 gccgtcgctg catcacgagt ctgctcggga gcgccccttg ggcgggcacg aagctgaggg
    30121 ggaactaggc gaccaccagc gcctgaggag cagcttgttt ggtacgtgtc tgcagccaag
    30181 cgagttggcg atcggtctga gttatatgcc tagtgcagag atgctccaaa tcctcatccc
    30241 gtaatgcccg cgacgcctgc agcagcaccg tccacgaaat catggcttcg gtggccagga
    30301 gccagaggtc atgcaagtct ctcaggagcg cgagccctcc ggttcgaggg ccttgaaaca
    30361 gatcactgaa gagtgtctcc ggctcgttca ccctcggttc cccgtaggtg gcgataaacg
    30421 gacggatccc gttcgatttc tctttggacc aggatgacag gaggcgacag gtctcctgca
    30481 catcaggctc ggcccgatga tggtcggaca cctgcaaaaa ggcctcagcc agctgcgttt
    30541 cgctgtggtg agctaaacga acatagacgc cgatattcat ccgaatgtct ttctgacaaa
    30601 tgtcttcacc agcttcggca caaggccgga cttgccgggc tttccaatac gaacggcggc
    30661 acatttgaaa tgcggctgtt tgctcaccgg atcccattcc accagcgtca attcattcgc
    30721 ggcgcgagga tgactgttct cgtcccaata gccgtagtga aacgggataa agagaacccc
    30781 tggtcgtatc ccggttatct tcgcccgcat ctgcacatat ccgcgacggg accgcacttc
    30841 cacgcgctcg ccgtccgcga tgccgtgatc tgccgcgtcc tgacttgaga tctccacaaa
    30901 cggctctggg gccttggctt ggagctcttc gacgcgcccg gtttttgtgc gcgtgtggaa
    30961 ttgatacacc acgcgtcccg tggtgagcca caacggaaat cgctcatcag gttcttcaaa
    31021 cggagctaga tagtcgctgg ctttgatgac cgcccgcccc tgcggattat gggcccgata
    31081 ccgttccggc gtcgccgcgg cacccgagtc aaagtcatgc ccgtaggtct cacagtattc
    31141 ggcgtcggag gggaaatgcc catcgacgta caaatgcgag gtcccatccg gattctcgct
    31201 gttacagggc cattgaatgc cggagcggct tgagagccgc tcgtacgtca taccactata
    31261 gtcgcagggg cgccctctcg agcatgcctt ccaggcttca aatgcttctt ccgctgtgtg
    31321 ccacttgatg agaggttggt cgtccttgtc tcgaaagttc atccgctcgg cataatccag
    31381 gaagatctcg agatccggac gggcttcccc tggcggctcg atggccttcc gggacaaatg
    31441 gaccgtgcgg tcgacgttgg taaagcatcc ggttttttcg ccccagatcg ctgccggcaa
    31501 aatgacatct gcccgcgagg ccgtttcggt cataaacgcg tcttgaacga ccacaaacag
    31561 atccttctga cccagaatac gcctggtacg ccggagatca ggcagtgaca cggcaggatt
    31621 ggttcccgaa atccagagaa atttgatgga accttgctcg caatagcgga agatctgcat
    31681 ggcatgtgtg gggggcgccc aatgtggaat gatatgaggc tcgacattcc agagcctggc
    31741 gaggtcggcg atatgtgacg gattatccca attgcgaaaa cctggcaaat ccccgtctgc
    31801 tccacactca cgcgtatttt gcgccgtggg ctgcccattc atctgataga ttccgcagcc
    31861 atcacgcccc agtagtcccc tgagaagatg gagattgttc acctgacagg ctgctgccgt
    31921 ggcctgcatg gattggtaga cgccctggag aacggtcgat acgagcgtcg gcgtggtgcc
    31981 gagaatttca gcggcttctc gcaggcctct ttccggtatg tccgccacct gcgcgacccg
    32041 tgcgggcgtc caggcggcca ccaccttttc cagttgctca aaccccatgg tatgggcact
    32101 gaggtacgag taatcgaccc atccatgttt gatgaggaga tgcagtaggc cattcataat
    32161 ggccacattt gtgcctggtt taggcgcgag atggacgtcc gctttcaagg cagccggcac
    32221 gggccgagga tccacgacga caagcttcgg cggattcgca ccgcaaagcc ggtccaacat
    32281 gcgcatccat aagaccgttt gttgctccgc gacattgtgg ccgaaattga aaagggcgtc
    32341 ggtcgtatca atgtcgttgt acgaacccgg ttgaccgtcg cagccgaacg tttctttgag
    32401 ggccgcggat gaggtggccg tacagaggcg tgtattgcca tccatatgtg gagtacctaa
    32461 accggctttt ccgatgacgc ctagcgtgta atattcttca aggaacagtt gcccgctggt
    32521 gtaaaagccg atagccccgg ccgtatactg ctgacgaact tcctgagatt ttcggacgat
    32581 cacatccatc gcctcgtccc aactgacttc ctcaaacctg ccctttttct gtatcagtgg
    32641 gtgtgtgagc cggtccgggc tcgcattggc cttccatcca tacagcccct tcgggcctaa
    32701 ccgtcctcga ttgacccgat cgtctgagcg tccccggata ccgacgattt ttccctctct
    32761 caccccaatt tccactccac acccgttgga gcacaacaca catgcggaag gcacccactt
    32821 ctccggagtt ccttccacat tacagtcaga ccgctccggc cactgtccgc gatagggcgt
    32881 gcgcgggccc caaatatcct cgatgctatt gcgggtaata gtcattgttc gtgaaagagg
    32941 tattcgcctc acttatacgc gcgaacatgt cccatatgat ctgggaaagc ccctagcgaa
    33001 cactctttac aaacgtttgc tcaggcagga caggcaatgg gcggagacgt ggcggcatca
    33061 ccactgacgg cgtagtagac atccaaccag gggctgacca tattgtgaag ggaaggaagc
    33121 agggtggtgg tgccggccag gattcggcag gaagatttca caactcgttc ctcccactgg
    33181 ccccacagag tcgtgtgctg gtctgacaat gtcatgagcg tatccacttc gaccgccacg
    33241 cgagagaaac catggcgtgc gaccagttga tccagagtcg aaaccgaata tccgttgagg
    33301 tagggaaaag acagaagatt attccacgca agggtcacga tagcccaacg acgccgacta
    33361 ccaccggcct tcaaccaatg cacgccttgc tgaaacatcg cgccgttagg aacccgcagg
    33421 accagatgac catcttcccg taggagccgg cgcgctgcac tgagggtcgt gtcgggattc
    33481 accaactgat caaaggtatt ccaaatgacc accgcatcaa ccgatcgggc ggcgatggtg
    33541 gcatcgttca gcgtgccatg aaagaccggc aatcctcggg cctggcaaaa attcaccacc
    33601 tctttccccg gatcaacgcc caggattgac caccctcttt gttgggcagc ggcaagaaaa
    33661 cccccgacaa aacttccaac ttcgacgact accggatgac gggaagtgaa gtgacagtgt
    33721 tgcgcgaagg ctgtaatctt ccactccgcc catctttgct gagcctgaaa ctccgcctcg
    33781 agatgctctt cgccatactg gtcgtctgca tactgagcgg tgacggatcg aagcgtcggc
    33841 cgaggattac gatagacaaa tccacacgtc agacatcgga ccacctccgt tgaatagtct
    33901 tgcgagaatg tggttcggtc cttgaggtcc gcctgcgcgt cctgacgttg gcgctgttgg
    33961 tgaaaccttc gcagcagctc tttttgttcg gcgatctctg ccgcaaacgc gaccgtttca
    34021 tgatagacac ctccgcatat caggcaggac acgtcgacca tgactggttc gtccgatccc
    34081 gcccgccgta ctgattcttc agctgttcct accccatgct gactcctgtg tagatcccgg
    34141 attgtattgc gttcagaaaa atactcatgg tttttcgcat gtcggtgagg aaagcatagg
    34201 cgcggccgtt caacattgga tattcagggc aggatcacag aacaatggcc ccacagtcgg
    34261 gggctgcata taacctcggg aaggatcttg tgctcccatc ggcgcattca gtggtggccc
    34321 tccctgcata cccggcaagc cgcgtcccgc gctggtgaaa ggcggagggc cactgttcgc
    34381 gcgaggcccc gagctgggcg cgagctggga ggtggtcgag ggtgtcagaa tgttagccgg
    34441 tagcccgctc gtcggcgctg atgaaggttg gataggagag atcgccgctg gagtgctcga
    34501 ttgaggagta cctaatccgg tttgcgccca agcgagccac gcacccccga ccgggcggga
    34561 acacccaacc gctatcatga gtaggaacag taagcgtttc catctcatgc tctcttatgc
    34621 catgcacatc tacatactga gagctaggat gttccctggt gatacggaga gccgacgtag
    34681 agcagcgaaa catggcaccc tctggcgcgc tagtaagggc cggcgaaggc gaggaagcgg
    34741 cgacttttgt ttatggtgac gaggagtgaa aaaactaaat cgtctcggtc tcgaaaggga
    34801 ctacaggctt gtttcgagta agggtggtga gtcggctggg attcgaaccc aggaccctcg
    34861 ccttaaaagt tcgttaagt
//
LOCUS       DAOQEW010000015        34563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_264_length_34563_cov_10.430654, whole genome shotgun sequence.
ACCESSION   DAOQEW010000015 DAOQEW010000000
VERSION     DAOQEW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_34563_cov_10.430654"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..806
                     /locus_tag="PLS35_03110"
     CDS             27..806
                     /locus_tag="PLS35_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNI17672.1"
                     /translation="MVIEPFYVYYKNNYGSSATAGVGNQAFGLGTAKHANQTRHMIGN
                     RIEMRKGGFDFSNEVAWQFGQMGQAGACVGDQKCLHINAWATRNWIGYTFYDTAWKTR
                     LAFNLDYASGDSRNNTCTNGTNCKTANTFENFFPTNHIHMGYMDVQAWKNMLSPSVNL
                     QARPSARDHIELWYTNLNLANSKDCWYRASQGCYVFSNANNTKTHIGDEIDVAYTRMF
                     ADGKVALQAAYGTIFSGGYLTSTLNQQVNQHWAYMSLWMNF"
     gene            886..1314
                     /locus_tag="PLS35_03115"
     CDS             886..1314
                     /locus_tag="PLS35_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17673.1"
                     /translation="MKKLMFVTLGVAALAIGSQPVTAQAQVADAIQAVNDAIVELTDA
                     PGLGKRTTVDVTKRWGSDRSVIDSATAKLQDGLSKAQSGGVGADAMRQLKLAVDYGKA
                     RMHKEARLSAQGALRHLCVANQDQGPGCDTVPKFGSYTAP"
     gene            1566..>2035
                     /locus_tag="PLS35_03120"
     CDS             1566..>2035
                     /locus_tag="PLS35_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI17674.1"
                     /translation="MNDLSTQLHQQFGFSSFRPGQREVIDAVLSRRDAMAVMPTGQGK
                     SLCYQLPATILPGLTLVISPLIALMQDQVNSLKARHVAAAAFHSGLSEPERDKIVLNL
                     KLRRLQLLYLAPERMQHERFLRLLRSLWVSLLVVDEAHCISQWGHDFRPDYLNIG"
     assembly_gap    2036..2135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2136..3067
                     /locus_tag="PLS35_03125"
     CDS             <2136..3067
                     /locus_tag="PLS35_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNI17675.1"
                     /translation="EAYYQEVGRAGRDGQPATCLLLFHERDVATQEYFIQQASKESGN
                     AARVERMKTLLQDLLDYVAGTTCRQLAILDYFSDEVERALGPCGLCDRCKTPVPLPVD
                     VVNDESACAKAILTAVSWCGGRFGVTRIVEMLRGSRSKGLLAYGAEDYPGYGIYQTWT
                     KTALTRLVKSLIDTGHLQVEGSEYPTLDLTRRGQEVVTGLRPVEVKTAVQSPASSAYQ
                     TPVNGPNNSMAASLTSSVDPQLFERLRQLRKELAEEEGVAPFVIFHDKTLRTIAGHKP
                     VTLSALLEIPGIGEVKVERYGRRVLGIVNEECR"
     gene            complement(3111..3731)
                     /gene="thrH"
                     /locus_tag="PLS35_03130"
     CDS             complement(3111..3731)
                     /gene="thrH"
                     /locus_tag="PLS35_03130"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HNI17676.1"
                     /translation="MQNPVIVCLDLEGVLVPEIWVNFAIKTGIEELKITTREMPDYDA
                     LMTRRLNILDQHGYTLADIQSVIDAMGPMEGAAEFIAWLRERAQVIILSDTFYEFALP
                     LMRQLGFPTIFCNQLEIGPNGRIVSYKLRQPNQKKHAVAALKSLNFRVMAAGDAYNDT
                     AMLGEAHAGFFFRPPAHLPKEFPQFPVTTTYAELQEQFRKAGGLKA"
     gene            complement(3822..4619)
                     /locus_tag="PLS35_03135"
     CDS             complement(3822..4619)
                     /locus_tag="PLS35_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNI17677.1"
                     /translation="MTHQTAVEHFEISLQTPVGEVSTAIGVPTSFVPVTAILPLMRGL
                     GEEVQTLELAHVRDAGQTVSCQKGCAACCRMLVPISAPEAFALAMSIDRLAPPERERL
                     LSKIDVAQQRLARAGILQQLSALAESSDPISDEAIEPLNRAYYALRMPCPFLDNEACS
                     IYDDRPAACRELAVTSPAADCQDMTNQTIRPVPVAVRISTALSLLWADLTATTPRLIP
                     LPLAIDWAKRHQAEQARQWAGTELFEKAIDKIGRYLGQERARRGSGG"
     gene            4821..5150
                     /locus_tag="PLS35_03140"
     CDS             4821..5150
                     /locus_tag="PLS35_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17678.1"
                     /translation="MRDLVEQARQESQAQHTFGFSAPPYQPDHAIADLLSILDDRIES
                     EGLQVGMPLEFLHEMWRHCNEARSQVMEQVWLESNLGPPGSSKDRIRELTYAALITHL
                     ESDAQQE"
     gene            complement(5183..6001)
                     /locus_tag="PLS35_03145"
     CDS             complement(5183..6001)
                     /locus_tag="PLS35_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNI17679.1"
                     /translation="MDLTPMWFGVYKASKYLLYPLSWIVLSGLLTLLIASLPVTPRRT
                     TWVRRLAFSTVLLLFLTATPLLSHLYISQLEARYPPFAVPPVAKFDAVVVLAGGVFHK
                     GSLRPADETSDPSRQRTACGAELWFQGLAPKMVLAGGDATVLRTGPPESREMKRWAIR
                     LGVPESVIHVEENSRTTYENAVQTRALLGPAHILLVTAAYHLPRAVGLFKKQGFTVTP
                     VACGYESTHHPQQAWEEKTVFDFIPTARALLLSTQTIDEVAGILVYWITGKWQP"
     gene            6093..6575
                     /locus_tag="PLS35_03150"
     CDS             6093..6575
                     /locus_tag="PLS35_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNI17680.1"
                     /translation="MAEGRHYWLMKSEPNVFSLDDLARAPKQITSWDGVRNYQARNFM
                     RAMKVGDQVLFYHSNAQPPCVMGIAEVVKTAYPDATQFDPHDVHYDPASPADQPRWDV
                     VDIRFVRKFAAPLSLDLLREQVRLKGMELLRKGSRLSVQPVKPSEWDEILRLARTHRD
                     "
     gene            complement(6632..6877)
                     /locus_tag="PLS35_03155"
     CDS             complement(6632..6877)
                     /locus_tag="PLS35_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNI17681.1"
                     /translation="MRIPNKVVLPFGYHIMIRQVTDSEMDRQDANADGIWDNEAKTIY
                     IRKRLPVTRRRYILAHELGHAWLDWQHRYLDDGKARS"
     gene            7073..7858
                     /gene="ricT"
                     /locus_tag="PLS35_03160"
     CDS             7073..7858
                     /gene="ricT"
                     /locus_tag="PLS35_03160"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNI17682.1"
                     /translation="MNDRMVYHPAMVDKLLAQELAKPYPSVIRIVGVKIRDRGEVKKF
                     DAGEATLAVGDRVLVEVGGELSYGVVYGAPQATPFIPPMRVIQSIARKATAEDAATID
                     RYERLASEGMRACREQAVALGLKMKLVEVFCSFHRRQMTFVYTAEDRIDFRELVRLLA
                     RRFGGRIEMRQVGVRDEASRLGGIDTCGLVLCCAAFLTEVKPVTVKQARVMGLPVDDP
                     KLLGVCGRLKCCLLFEAMESQPSSSSSKSSALINPARTRLASS"
     gene            7978..9069
                     /locus_tag="PLS35_03165"
     CDS             7978..9069
                     /locus_tag="PLS35_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNI17683.1"
                     /translation="MPQVLVLGSGKIGSLIAGLLSAKGQYQVHLADLTLDAPKRLIDD
                     LALTTVTPCALDVRRPDSVVAYVTAHRFDAVISCLPYFYNPAVAEIARAHHLHYFDLT
                     EDVAVTGCVKRISEGADHAFLPQCGLAPGFISIVTNDLIGHFESIDHVKMRVGALPVH
                     PSNALKYSLTWSTDGLLNEYGNVCVGIERGEEVHLRPLEGYETIELDGLLYEAFNTSG
                     GLGTLADTYRGRVRTMNYKTLRYPGHCEKIQFLMNDLKLNEDRETLKRILERAIPQTH
                     QDVVLMYAAVTGTRQGELFEETYVKKVYPRMMLGRTWSAIQVTTASSLCCVLDLVLAQ
                     PSAYRGFIRQEHFPLRDVVNNRFGDCFRS"
     gene            9077..10633
                     /locus_tag="PLS35_03170"
     CDS             9077..10633
                     /locus_tag="PLS35_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNI17684.1"
                     /translation="MTTEELFTRLGLAREQSGGGGSSSTWTRTTPAGVVESRNPSTGQ
                     TLGRVYGCTDDEYDALVIGLQHMQKVWRTVPAPKRGEVIRRIAEALREQKDALGSLIA
                     LEVGKIKAEGDGEVQEMIDMADFAVGLSRMLYGYTMHSERPQHRMYEQWHPLGVIGVI
                     TAFNFPVAVWAWNAFIAAVTGNTVLWKPSPKAPLCAVAIQQLCNRVLEDAGYPGVFAL
                     CTTDRVELAERLVRDERLPLISFTGSVPVGRQVAEVVGRRLGRSLLELSGNNAIIVDE
                     TADLELATRAIVFGAVGTAGQRCTSTRRVIVQDSQYDKLVSRLVSAYGQVTIGDPLQP
                     NVMMGPLIDREAVARYQSALEDVRQAGGEVLCGGRVLSRPGYYVEPTIVRAQNHWKIV
                     QQETFAPILYVMTYRTLDEAIDMQNAVPQGLSSALFTTHLHHSEAFLSASGSDCGIAN
                     INIGTSGAEIGGAFGGEKHTGGGREAGSDAWKAYMRRQTTTINWGTELQLAQGITFGR
                     SEGGNHGASR"
     gene            10617..11210
                     /locus_tag="PLS35_03175"
     CDS             10617..11210
                     /locus_tag="PLS35_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17685.1"
                     /translation="MGQAAELDAVIARMTADFLAQNTAAGMLRRALDDVGVGFVPVMD
                     HLTIRTMNIDQRAEEFVALGYGYDETIYYDDWYAKVFRKAGYPALFVDQAYPDARGHT
                     SIIPRWVDKFGDRVFHHVAVRVEDIEQAILKLKSKGVVFAGEIVGARGGPLRQIFTAP
                     EMIDGQPFSVLELAERHQGYQGFLPPQADSLMRSTVR"
     gene            11363..12433
                     /locus_tag="PLS35_03180"
     CDS             11363..12433
                     /locus_tag="PLS35_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNI17686.1"
                     /translation="MVRTVLPLCFILVLLCVATFGIGESLVGASPSGHAPHGTVTIDG
                     ITVPDIGPLPTAVPIPSSNVNYAAKIELGKQLYFDGRLSKNNAISCAFCHNPGTGFAD
                     SRQTSIGVGGGIGGRQSPTVYNTGFNHVQFWDGRARSLEEQAIGPIHNPVEMAETHEH
                     VVAKLGKIKGYQQEFRAVFGTEVNLQGIAEAIAAYERTVLSTNSAFDKYVLGDVKAMD
                     EAAARGLGLFKGKARCILCHNGPNFTDNQFHNLGVPQVGPMKEDLGRYNVTRADKDKG
                     AFKTPTLRSVTETAPYMHDGAFKTLEEVVDFLDQGGGSNPRLSPLMRPLGLTTEEKAD
                     LVMFLKALTGEPIKFSMPQLPK"
     gene            12544..13050
                     /locus_tag="PLS35_03185"
     CDS             12544..13050
                     /locus_tag="PLS35_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNI17687.1"
                     /translation="MFRQLCVLGASTLALAMGGPALAADMKPGPADGFDIHVMAPHKM
                     EDGSVAGPFHHYCKAIKPEVLQCLLFESTAPNAVLTDVEYFVAKPVSRSAVPLDVWNK
                     FYHDHEVEIATGRVQVLDMPDAQAKEVAAAAAKTDGIIFHLWPKGAKAPDGSVGHPTS
                     VGHKPRTE"
     gene            13104..13658
                     /locus_tag="PLS35_03190"
     CDS             13104..13658
                     /locus_tag="PLS35_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HNI17688.1"
                     /translation="MRSAIAMIGLCAAALTATGCAETSHDMKSTAAAAPAATAAKAMP
                     TPAQGYTIHVMAPHKFEDGTVHGPYHHYCKPISPEILQCLLFESTDSNALLTDIEYFV
                     AKSVSRAHVPLETWNKYYHDHEVEIATGRVQILDMPDAQAKEVAAVAAKTDGIIFHLW
                     PDGAKAPNGEVGHPQSVGHKHRTE"
     gene            13743..14189
                     /locus_tag="PLS35_03195"
     CDS             13743..14189
                     /locus_tag="PLS35_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI17689.1"
                     /translation="MKSVSLFLYAWTVVGVGAIAWSADSEVLRPRVPVDQIESARAVT
                     NPFPVTPEMREKGKALFEGKAFCRACHGADGKGLGLDLDYSTFKGPLPRNFTDKMWQQ
                     ARTDGELFWILKNGSPGTDMASFIPLVLTEEEAWQVLMYVRSFGGR"
     gene            complement(16918..18036)
                     /locus_tag="PLS35_03200"
     CDS             complement(16918..18036)
                     /locus_tag="PLS35_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17690.1"
                     /translation="MPSADAPSLVIEQGTCYALFAYDIGLAINLDEAERHVTAIKERG
                     RMRHKARAPHYFEYRPAPLRLIQETASLDFGDYRSSEAVEVMLYDFGAVTVIYRIPIN
                     GPFEGLLSLSESLYENERLLADSRHRLDQLVRDIRLAIDRPSVSPEVEDYLVFSVEHC
                     SPSAIQALGTPFEPMFAHVLRSERTRLSDQEIREATSCRISFSRDDTALIDWNAAIVF
                     GEDMDDVRAVLEFVNVELLEMRTLDEQLDQALDQGYVALTRKARVTSWLPGSHEAALT
                     QIGQLQVDSAVLFERVANTLKLLGDQYLARVYRLASQRFHLEAWDASIIRKLQTLDSI
                     YGKMSDRATTQRMEVLEWIIIALITISIAVSFLPMTSH"
     gene            18247..18924
                     /locus_tag="PLS35_03205"
     CDS             18247..18924
                     /locus_tag="PLS35_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNI17691.1"
                     /translation="MPTIKAIIFDFDGVLFDTEAIHFAVFREVLRSEGIPLTLSRYHA
                     AYVGLSDEACFRAILNDHRIVVPKEDIQQLVRRKTDLVQRAFRASLPRVSGVSEFVSD
                     ASMRYRLAIASGALLQEIVLCLKLGSMLTMFEHITAAGDVREGKPNPAPYLHALHALH
                     GRSPIAANECLVIEDTPHGVEAAHKAGMRCVAVTTTLPADRLAQADLVVPSLHALRLE
                     DLTVQLP"
     gene            19062..21464
                     /locus_tag="PLS35_03210"
     CDS             19062..21464
                     /locus_tag="PLS35_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17692.1"
                     /translation="MSRTTALSANSSPYAVESRHVIEREAVEFLANSVTLPFSSSDIT
                     AGSESELQAAVLGRSADVDLAQTIHRSNYLKNIRDRTETGESPRIVIQQLQEFLNDSQ
                     STVWENSWVRFPRNMLNKEANQLLACDLRANRRDPKGTPRTDIDRFLVTEQGEEWVRV
                     PVSYVLRLALAQVIGAIESDVPMLAKEGRRLLDHFLNDNSSPETFSFYVSGMDPARGM
                     GKAIAHETAIRFLLTQLLVQYANRAFGLDETGQHAIVYASPHPPVRQRRLNGLISDSF
                     YRELFVNPCLSGWDQGEDKSRYMGLCHEVLSRSQLNAVVRLKDAGIITRNLVVLPATS
                     NISLANNGTHLSLGSRMLTETLQANGKRFTAVHEKVLGDLVIKIVEHFLPLFVGTYSA
                     APYRMNFRDFHPETALGFLPHELDFAHLRMLWRRWKKKAGLSVFGRSITPLGPPWLDR
                     AISFIFNLRGDCLPDFRLIDYLVCLLSTEQSPALDGIPGNDLRLKRDLGQLGVFDERM
                     SLYLLYKLRAQSVMGFSGFEGRHYSLFWNHTDDFAQAASMQALVTALAFQYIARGEVS
                     HEDIPDDPMTESERRQYLFATAVGVGTVNVREAGPNRFLTRILMKTHKTRASRRYAGH
                     LRVRCEDYRMALVAMLEEDASDLIESFQMQECMRSLRDRLSNQDSSTAGKLTHGILNS
                     VGSPSAFDLSGEEFALAAEGYYRRELRAQYLREACESVREELCAIDQGVSEQATQYRG
                     LVTAMIGTRSAVDAFTEEQPRLLAETADAKTLQRCLGLCIMSIAHARISAGRTEEQPL
                     AS"
     gene            21461..22486
                     /locus_tag="PLS35_03215"
     CDS             21461..22486
                     /locus_tag="PLS35_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNI17693.1"
                     /translation="MSMPHQYIERDSNLVVTERFCGDQWLNWAYRALWENANWFMKAL
                     TSRRTTQALGFFNYDRPFTSTQAAVARFARALSVDLSECLDSSDRLNTPRRLFQRRLR
                     YWQVRPMDDDPRAIVSPADARLLIGSFADDSSIFLKGKFFDYDELLGKHRPQWRRLFQ
                     RGDWAIFRLTPDKYHYNHCPVSGRVVDCYEINGRYHSCNPGAVVVAATPYSKNRRIVT
                     IIDTDVPGGTRVGQVAMIEVVALMIGQIDQCYSSVAYDDPQTMQIGMMLKKGCPKSLY
                     RPGSSTDVLLFEPGRLSFSEDLLRNRFRPGVQSRFSEGFGRPLIETDVKVRSTIAWRL
                     ERGIDGR"
     gene            22476..23531
                     /locus_tag="PLS35_03220"
     CDS             22476..23531
                     /locus_tag="PLS35_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17694.1"
                     /translation="MGDSTSVIALLIVLYGLLFWWAFRTLPQESWQFVASMPRMKLSD
                     GRWVGTNLTYYGAWTATAVVFAVAMAIVLMQSVAVALDAIALLAVVLLGLCVPASKIL
                     ARLIEGKANTFTVGGASMLGLFLAPMIIDGINLVLNWTGKGALPAAAVLAALSVAYAF
                     GEGTGRLACLSFGCCYGVPLQESGPRLSAWFQQYHIAFVGRTKKAAYEGDLEAVPLIP
                     IQALTAVMSVGAGITGVWLFLQGHMAASFLSTIAITQLWRVASEYLRADFRGVGTLSW
                     YQILSLLGVLYAFVLTFWLDTFSPSVPNMRLGLRDIWSPGIILALEALWLGIFFFTGR
                     SRVTASTLTMRVLRDRI"
     gene            23735..23920
                     /locus_tag="PLS35_03225"
     CDS             23735..23920
                     /locus_tag="PLS35_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17695.1"
                     /translation="MNIADDLFNDEERKQLAYEAASAMAKRALTIDERSAHAHFLYAA
                     SLGSGEASKGFRKVGLS"
     gene            23986..24258
                     /locus_tag="PLS35_03230"
     CDS             23986..24258
                     /locus_tag="PLS35_03230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17696.1"
                     /translation="MMGGLCAELPWLLGGSDKDAELYLKRAIAADGRFTNAHLILARL
                     LMRQGRLAEAREHIESVLHAEHPHYPYAWKHTFRPEAERLLNLLSK"
     gene            24516..26123
                     /locus_tag="PLS35_03235"
     CDS             24516..26123
                     /locus_tag="PLS35_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNI17697.1"
                     /translation="MGPALHTTTAAHPLTVQPSRSLASFGFFAFVILLFLGTLYTVEQ
                     LSFELMAVKQSSAWPFILLGVALVIALGFEFVNGFHDTANAVATVIYTHSLPPHAAVI
                     WSGLWNFIGVMMASGAVAFGIVSLLPVELILQVSAGAGFAMVFALLIAAIIWNLGTWY
                     IGLPSSSSHTLIGSVIGVGLMNQLMAGSAEATSGVDWAQALNIGKSLLVSLLFGFSVA
                     AFLLLVAMRAIPIASLYRAPQGKTPPPLWIRGILILTCTGVSFAHGSNDGQKGMGLIM
                     LILIGTVPTVYALNRTVDISYTPAFVETSRQAERTLDRYTHGQPAPTDPRHEVGQYLR
                     SRQLMPETIPALRALSAEVREGISAFGSVAQVPDEQTGNLRNDMHLITEALRRMEKQH
                     DPPLSAPDLKILKEYRSTLDTATKFIPTWVKAAVALALGLGTMIGWRRIVVTVGEKIG
                     KAHLTYGQGACAEMVAMGTIGAADMLGLPVSTTQVLSSGVAGTMAANQSGLQWNTVRN
                     LLMAWVLTLPASIVLSGSLFWIFRHLV"
     gene            26153..27118
                     /gene="corA"
                     /locus_tag="PLS35_03240"
     CDS             26153..27118
                     /gene="corA"
                     /locus_tag="PLS35_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNI17698.1"
                     /translation="MLSAFVLEEGRVIQAQIDDKDDLTRKDLVWVDLAYPSEEERELV
                     QSVFPLELPDDEELQDLEASARYYQDQHGIHIRSTFVQTADDVASIVNMSFSLHQGRL
                     LTVHDDDLSLLRLFRMQARSSRGMVRDAADILLSCYELGVERDADVLERIYTALDEVG
                     TVVLSRKKEITSRIMRDNVERIAAQEDLNSKVRLDLIDNRRALSFLLRSRVLSPDQAQ
                     RVKGVLRDIDSLNGHTGFIFDKINFLMDALLGMINLEQNKIIKIFSIAAVVFLPPTLV
                     ASIYGMNFEHMPEMKWPLGYPMALLLMVFAGGAPYLYFRQKGWLD"
     gene            27159..27479
                     /locus_tag="PLS35_03245"
     CDS             27159..27479
                     /locus_tag="PLS35_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17699.1"
                     /translation="MNSQQNNKPSRPGRKGALAKLSEPQVGDAPPKLKSKPSKRPMLH
                     DASTVPPQKHVLTQEEFRARVSRKAFELYEIRRSVTEVDDWIAAERLVKVELLSEERG
                     AGSV"
     gene            28019..28339
                     /locus_tag="PLS35_03250"
     CDS             28019..28339
                     /locus_tag="PLS35_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNI17700.1"
                     /translation="MRTKTKQLTDVATVFHALSDETRLLILEQLKGGEQCVCDLTEIF
                     KTGQSRLSFHLRVLKEAGLILARPEGRWVYYSLNSDAVEELQDVVNSLKAVSSRAARQ
                     GCCP"
     gene            28383..29492
                     /gene="arsB"
                     /locus_tag="PLS35_03255"
     CDS             28383..29492
                     /gene="arsB"
                     /locus_tag="PLS35_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNI17701.1"
                     /translation="MKLMDAAGVIAQQTLPTEKRLNVFERYLTVWVAFCMVAGMLLGT
                     SAPGLIQMLRGMEFGQDSHVNFPMAVLIWLMITPMMMNVDFRAVAKVGQRPQGLLVTL
                     FVNWVVKPFSMALFAWIFFRFLFSAWMSPAEADQYIAGAIILAAAPCTAMVFVWSYLT
                     DGDPAYTLVQVSVNDLIMLVLFAPIVGLLVSGASSLTVPLAVLVYSVIGFIVIPLVLG
                     VVIRHWFISQRGKTWFEETLLPSFAPMTIGALLVTLVLIFAFQADNILAKPLHVLLIA
                     IPILLQVYFNASLAYGLMKWVKVPYAVAAPGALIGASNFFELAVATAIALFGPESGAA
                     LATVVGVLVEVPVMLSVCHVCNRTRHWFPSKEATA"
     gene            29909..30379
                     /locus_tag="PLS35_03260"
     CDS             29909..30379
                     /locus_tag="PLS35_03260"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HNI17702.1"
                     /translation="MKRFHLHMGVEDLDTSIRFYTALFGVPPVKTKSDYAKWMLDDPR
                     INFAISTRAKKGVDHLGIQADGEDELAALRGRIQAGHLPVVNEGKTVCCYALSDKSWI
                     QDPSGLPWETYRTMEDAQLFSGSTDSSEGACCTPLTMAPTNCCEPSEKTAGCCD"
     gene            30379..30807
                     /locus_tag="PLS35_03265"
     CDS             30379..30807
                     /locus_tag="PLS35_03265"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNI17703.1"
                     /translation="MESAIVTVLVLCTGNSCRSIMAEALINALRPGRYHAVSAGSHPT
                     GYVHPKSIDTLKRHGIDPGQPKSKSWNEFSHQTFDLVITVCDQAAAEACPLFLGAPTK
                     MHWSTPDPAKATGSEVEIDAAFDQAFLMLKKRVEHLTSTT"
     gene            complement(31197..32330)
                     /locus_tag="PLS35_03270"
     CDS             complement(31197..32330)
                     /locus_tag="PLS35_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNI17704.1"
                     /translation="MDQKTLLDLANLHGTPLVVVDHAVLRENYDQFRKHLPRVQVYYA
                     VKANSDPAIVQTFYDVGASFDVASMAEFLIVHEKIKHLPAQQRQDFIWDRIIYANPIK
                     AIETLTQLDQYKPLITYDNHEEVLKIARYAPHSGLVLRLSVPNTGSMVELSSKFGALP
                     GEAVDLIAFAHNNKLVVEGLSFHVGSQCTNVQNYIQALHLAAGIFSEAKTRGFDLKLL
                     DIGGGFPAHYDDSVPAFQSLAKTINAELDRLFPQPIEILAEPGRFLVASAATSVAQII
                     GKAVRGGKLCYYLDDGVYHTYSGVIFDHCQYHLKSFRKDPTQICAVFGPTCDALDTIS
                     LVEQLPDMDRGELVYAENMGAYCGASSTYFNGFPPAKVVHVNQ"
     gene            complement(32408..33868)
                     /locus_tag="PLS35_03275"
     CDS             complement(32408..33868)
                     /locus_tag="PLS35_03275"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNI17705.1"
                     /translation="MQVFPNRILFVGFGAVARCTLPILLKHIGVDPKRITILDFDPNE
                     AALAPWIKQGVTFVKDRVTPENLGTLLGKHASRGDLLIDLAWNIDCCEIVQWCHDRGV
                     LYINTSVEIWDPYCGVDDKHPTERTLYWRHMNLRRMMASWGEPGPTAVVEHGANPGLI
                     SHFTKHALLDIARQTLADKKFSGEQAERIAHHAQAQEFNHLAHQLGVRVIHCSERDTQ
                     ITDRPKQVDEFVNTWSVEGFREEGTTTAEMGWGTHEKQLPRFAYEHPDGPKSQICLAR
                     MGMNTFVASWVPPDHNIIGMVVRHGEAFSITEKLTVWEGDKAIYRPTVHYAYCPCDAA
                     IASLHELRGSDYRLQSRTRIMTDEIISGADILGVLVMGHPYQSWWCGSHLDIEEARRL
                     VPHQNATTMQVAISVVAACMWMLENPSRGLCVPDNLPHDYILNMSKPYLGKFISAASD
                     WNPLKHYSNFFRGYNQPQLDRSDPWQFKNFLIMEGD"
BASE COUNT         7525 a         8901 c         9999 g         8038 t
ORIGIN      
        1 acaatcagct gaaaatgatt cctggaatgg tcattgagcc gttctacgtg tattacaaaa
       61 acaattacgg ttcatcggct acggcagggg tcggaaacca ggcatttggg ctgggtaccg
      121 caaagcatgc caaccagact cggcatatga ttggtaatcg aattgaaatg aggaagggtg
      181 gattcgactt cagcaatgaa gttgcctggc agttcggtca gatggggcaa gctggcgctt
      241 gtgtgggcga ccagaaatgt ttgcatatca atgcgtgggc gaccagaaac tggattggct
      301 acacgttcta tgacacggcg tggaagaccc gcctggcctt caacctcgat tacgcgtctg
      361 gcgacagccg gaacaacacc tgcaccaacg ggaccaattg taagacagcc aacacctttg
      421 aaaacttctt cccgacgaac cacatccaca tgggttacat ggacgtgcaa gcgtggaaga
      481 acatgctgtc tccttcagtc aacttgcagg ctcggccttc ggcgcgtgat cacatcgaat
      541 tgtggtacac gaacctgaac ctggccaatt cgaaggactg ttggtatcga gcctcgcagg
      601 ggtgttacgt gttctcgaac gccaacaaca cgaagaccca catcggcgac gaaatcgacg
      661 tggcgtacac ccggatgttc gccgacggca aagttgccct gcaggctgca tatggcacga
      721 tcttcagcgg tggctatttg accagcacgt taaatcaaca ggtcaatcag cactgggcct
      781 atatgtcgct gtggatgaac ttctaggaaa aagcgatcag gttacagctt tcagctgaca
      841 gcgtccttgc tgatagctga tggctttcac caaaggagac acgcaatgaa gaaactcatg
      901 ttcgtaacgc tgggcgtcgc agcgttggcg atcggcagcc agcctgtcac tgcacaggcc
      961 caggtcgcgg atgcgattca agcagtgaac gatgccatcg tcgagttgac cgatgcgccg
     1021 ggcctcggca agcggacgac cgtcgatgtg acgaagcggt ggggttccga ccggtccgtg
     1081 atcgacagcg ccacggcgaa attgcaggat gggctcagca aggcccagtc cggtggagtc
     1141 ggcgctgatg cgatgcgtca gctgaagctg gcggtcgatt atgggaaagc ccgcatgcac
     1201 aaggaagctc gcttgtccgc acagggtgcc ttgcgccacc tgtgcgttgc gaatcaagat
     1261 cagggcccag gctgcgacac ggtgccgaag ttcggcagct acaccgcgcc ataggtagaa
     1321 ctctggtgat cttcgaggta tgaggggaga tccccctgtc tgtctcgagt ctgaaacccc
     1381 gccgggtgat ccccggcggg gtttcttttt tgaggcgact gactcttcgc tgatgtcttc
     1441 ggctgatgat tgatattcga tgtaacctga cggcgagcga ctcctgctgc ttgcccgtga
     1501 ttcgtcggac gtcttatact cggccgtccg caatacgcaa agtagttccg ttctgagtgt
     1561 tccccgtgaa tgacctaagt acacagcttc atcaacaatt cggattttca tcattccgcc
     1621 cgggccagcg ggaggtcatc gatgccgtct tgtctcgacg cgatgccatg gcggtgatgc
     1681 ccaccggcca gggcaagtca ctctgttatc agctgccggc tacgatcctg cctggcctga
     1741 cactcgtcat ttccccgttg atcgccctga tgcaggatca ggtgaacagt ctgaaagccc
     1801 gccacgtggc cgccgcagcc tttcattccg gcctctctga accggagcgt gacaagattg
     1861 tcctcaatct taaattgaga cggctccagc tcctctatct cgcccctgaa cggatgcagc
     1921 atgaacggtt cctgcgtctt ttgcgatcgt tatgggtgtc tttgctggtc gtcgacgaag
     1981 cgcattgcat ttcgcaatgg ggacatgatt tcaggccgga ctatctgaac atcggnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggag gcctattacc aggaggtcgg
     2161 acgggccggg cgtgacggcc aaccggccac ctgcctgctg ttgttccatg agcgggatgt
     2221 ggccacgcag gaatacttta ttcaacaggc ctcgaaagaa tccggcaacg cagcccgtgt
     2281 ggaacgcatg aagacgttgc tccaggattt gctggactat gtggccggga cgacctgccg
     2341 ccaactcgcc atattggact atttcagtga tgaggtggag cgggcgctgg gcccttgtgg
     2401 attgtgtgac cgttgcaaaa cgccagtgcc gttgcctgtg gacgtcgtaa atgatgaatc
     2461 ggcatgtgcc aaggccattc tcacggccgt ctcctggtgc ggggggcgat tcggagttac
     2521 gcggattgtg gagatgctgc gcggcagccg gtcgaaaggg ctcctggcct atggggctga
     2581 agactatccg ggatatggaa tctatcagac ctggaccaag acggcactga ctcgtctggt
     2641 gaaatcctta atcgacaccg gacatttgca ggtggaaggg tcggaatatc ccacgctcga
     2701 cttgacgcgg cgcgggcagg aggtggtgac gggcctcaga cctgtagaag tgaagacggc
     2761 ggtgcagtcc ccggcttcgt cggcgtatca gacgccggtc aacggcccaa acaactccat
     2821 ggccgcatca ctcacgtcgt ccgttgatcc gcagttgttt gaacggctcc gtcaattgcg
     2881 taaagaactc gccgaggaag agggcgtggc gcccttcgtg atttttcacg acaaaacgct
     2941 tcgcacgatc gccggccata aacctgtgac gttgagcgcc ctgctggaga tccccggcat
     3001 cggcgaggtt aaggtggagc ggtatggccg gcgcgtgctc gggatcgtca acgaggagtg
     3061 tcggtaactt cttccgtgtt gtatcgtccg cgtttcattc tgcgttctta ttaagccttc
     3121 agccctcccg ccttacggaa ttgctcctgc aattccgcat atgtcgtcgt cacaggaaat
     3181 tgcgggaatt cttttggcag atgagccggg ggacgaaaga agaatccggc atgggcctct
     3241 cccagcatgg cggtatcgtt gtaggcatcc cctgcggcca tgacgcggaa attcaggctc
     3301 ttcagtgcag ccaccgcgtg tttcttctgg ttgggttgcc ggagtttgta gctgacaatg
     3361 cggccgttgg ggccgatttc cagttgattg caaaaaatgg ttggaaagcc cagttggcgc
     3421 atcagcggaa gggcgaattc ataaaacgtg tcagacagga tgatgacctg agcgcgttcg
     3481 cgcagccagg cgataaattc ggcggcccct tccatcggcc ccatggcatc gatgaccgat
     3541 tgaatgtcgg cgagggtgta gccatgttga tcaagaatgt tcaaccggcg cgtcatcaag
     3601 gcatcatagt ccggcatttc ccgtgtggtg atcttgagtt cttcaatgcc ggtcttaatg
     3661 gcaaagttca cccagatttc aggaactaag acgccttcaa ggtcgagaca cacaataacg
     3721 ggattctgca ttcattcctc cggaaaagtc gtacatgtga atagtgatgg gacgagggag
     3781 cgctcgcaca cgtcacagcg aagaaccccc aaatctgtct gttatccgcc gcttccgcgc
     3841 cgagcccttt cctgaccgag ataccgcccg attttatcga tcgccttttc aaatagttcg
     3901 gttccggccc attggcgggc ctgttcggcc tgatggcgtt tggcccagtc gatggccagc
     3961 ggcaggggga tcagcctggg tgtggtggcc gtgagatccg cccagagtaa actcagcgcc
     4021 gtgctgatgc gaaccgcaac gggaaccggc cggattgtct gattcgtcat gtcctgacag
     4081 tcggcagccg gggaggtcac ggcgagttcc cggcaggctg ccggtcgatc atcgtagatg
     4141 ctgcaggcct cattgtctag aaaagggcag ggcattcgaa gcgcataata ggcccggttc
     4201 aggggttcga tggcttcgtc actgatcggg tctgaagatt cagcgagtgc ggagagttgt
     4261 tgcaggattc cggcgcgggc caggcgttgc tgagccacgt caatttttga caggagccgc
     4321 tcacgctccg gtggggccag gcgatcgatg ctcatcgcga gagcgaaggc ttcgggcgcg
     4381 gaaatcggca ccagcatgcg acaacaggcc gcgcagccct tctggcagga aacagtctgc
     4441 ccggcgtcac gcacatgggc gagttccagc gtctggacct cctcgcctag tccgcgcatg
     4501 aggggaagga ttgccgtcac cggcacgaag cttgttggaa ctccaatggc ggtggagacc
     4561 tcaccgaccg gggtttgcag ggagatttcg aagtgttcga ctgctgtctg gtgagtcacg
     4621 gttctcgcgt cagctcattt cttgcggtca tcatcggaga taaaagcccg tatgggcgtt
     4681 gctgcagcta tcatagagaa ccatagaatc ggcggtcaac caatgggctt ggacgccggt
     4741 cttgccccac ggggacggtt gcggctactc tgtcgtcgat gcgggtatcc ggccatatca
     4801 tcggtttgtt aaaagaatat atgcgtgatc ttgtcgagca ggcgcggcag gaatcgcagg
     4861 ctcagcacac gtttggcttt tccgcaccgc cgtatcagcc ggatcatgcc atcgcggacc
     4921 tgctctcgat tttagatgat cggattgagt cggaaggtct gcaagtcggc atgccgcttg
     4981 agtttctgca tgaaatgtgg cggcattgta acgaggcccg cagccaggtg atggaacagg
     5041 tgtggctgga atcgaatctt ggcccgccgg gttcatccaa ggataggatt cgagagttga
     5101 cctacgccgc attgatcacc catctcgaat cggatgccca acaagaatag ccgtgcccgg
     5161 tcgtcattcg tgccgaggac actcacggtt gccattttcc ggtgatccaa tagaccagaa
     5221 tgccggccac ttcgtcgatg gtctgcgtgc tgagtaagag tgcccgggcc gtggggatga
     5281 agtcgaacac tgttttctct tcccaggcct gttgaggatg gtgcgtggat tcgtagccgc
     5341 aggcaacggg agtgaccgta aagccctgct tcttaaataa gcccaccgcg cgggggagat
     5401 gataggcagc ggtgacgagc aggatgtggg ccgggcccag gagggccctg gtctgcaccg
     5461 cgttttcgta ggtcgttcgg gaattctcct ccacatggat gaccgattcc ggcacaccaa
     5521 ggcggatcgc ccaccgtttc atctcgcggg attcaggcgg tccggttcgg agtacggttg
     5581 catcgccgcc ggcgagcacc atcttgggtg cgaggccctg aaaccacaat tcggcgccgc
     5641 aagcggttcg ctggcgcgag gggtcggagg tttcgtcggc cggccgcaat gatcctttgt
     5701 gaaagacacc ccctgcgagg acgaccacgg cgtcgaattt cgctacgggg ggtactgcga
     5761 acggcggata gcgcgcctca agttgcgaaa tatagaggtg tgaaagaagg ggagtggcag
     5821 taaggaagag gagcagcact gtgctgaagg ccaagcgtct cacccaggtc gttcgtctag
     5881 gcgtgacagg cagagaggcg atgaggaggg tcaagagtcc ggacagcacg atccaggaca
     5941 agggatacag aagatatttt gaggctttat agacaccgaa ccacatcggg gtcagatcca
     6001 taggaactcc acggttggct caaagaattt ggctcaaaga atgctcagca tacacggagg
     6061 tgtgacggac accaagtgtt caggaggatg gtatggcaga gggacggcac tactggttga
     6121 tgaaatccga acccaacgtc ttttcactcg acgatctggc acgcgcgccg aagcagataa
     6181 cgtcttggga cggggtgcgg aactatcaag cgcgtaattt catgcgggcg atgaaggtcg
     6241 gtgaccaggt gttgttttat cacagcaacg ctcagccgcc ttgcgtgatg gggatcgccg
     6301 aagtggtcaa aacggcctat cctgatgcga cacagttcga tccccacgat gtgcattatg
     6361 atccggccag cccagccgac cagccccgct gggatgtggt ggatatccgg ttcgtgcgga
     6421 agtttgccgc gccactctca ttggatttgc tcagagaaca ggtacgttta aaggggatgg
     6481 aattgcttcg aaagggatcg cgcctgtcgg tacagccggt gaagccgtct gagtgggatg
     6541 aaatccttcg actcgcgcgt actcatcgtg actgaagcgg tacgtgaggg gcgaatggac
     6601 cccttcaaga tcttcattcg taaaggaacg gctaactgcg agcctttccg tcatccaaat
     6661 agcgatgctg ccagtcgagc caggcatggc caagctcgtg ggcaagtatg taacgccgtc
     6721 gcgtaacggg aagccgcttg cggatataaa tggtttttgc ttcgttgtcc caaatcccat
     6781 ccgcattggc atcctgccgg tccatctccg aatccgtgac ctgccggatc atgatgtgat
     6841 agccgaatgg aaggaccact ttgtttggta ttcgcaccgt gcccctcact gtctttctta
     6901 agggcgcctt ctgcgccgga ttcctgctcc cggatagcct aacatatttc ctgtttcgtc
     6961 ttccccgatt aaaaattcag ttttttcagg tgcttatgcg tcgttattcc gcctggtgtg
     7021 ttttccgctc ccctggtgtc ttcaggtgta aagagggtgt ggaggatttc aattgaacga
     7081 tcgaatggtg tatcatccgg ccatggtgga taaactcctc gctcaagaac ttgccaagcc
     7141 ttacccgtcg gtcatccgga ttgtgggtgt caaaatccgt gatcgcggcg aagtcaagaa
     7201 attcgacgcc ggggaagcca ccttagccgt aggcgatcgc gtattggtcg aggtcggggg
     7261 tgagctgagc tacggagtcg tgtacggggc tcctcaggca acgccattta tcccgcccat
     7321 gcgtgtcata cagtccattg cccggaaagc caccgcggag gacgcggcaa ccattgatcg
     7381 ctatgaacga ctggcttccg agggcatgag agcctgccgg gaacaggccg tcgcgttggg
     7441 gctgaagatg aaactggtcg aagtcttctg ctcctttcat cggcggcaga tgaccttcgt
     7501 ctatacggcc gaagatcgta tcgatttccg cgagttagtt cgtctgctcg cgcgccggtt
     7561 cggcgggcgg atcgaaatgc gtcaggtggg cgttagggat gaggccagcc gattgggcgg
     7621 aatcgacacg tgcgggctcg tcttgtgctg tgccgcattt ctgacagagg tcaaaccggt
     7681 gaccgtgaaa caagcccgcg tcatgggtct tcccgtggat gaccccaaat tactgggagt
     7741 ctgtggccgc ctcaagtgtt gtctcttgtt cgaagctatg gaaagtcagc cgtcctcctc
     7801 gtcatccaaa tcgtctgcac tgataaatcc cgctcgtacc cgtttggcgt cttcctagtt
     7861 ccccatccgt ttcggccaga tctctgtcgc atcgcgctgc gaagcctgct tttgcgacct
     7921 gctggtgaaa gcctcgtcat tgtagtatga aagagtataa tgaccatgag gtgacggatg
     7981 cctcaggtac tggtactcgg atccgggaaa atcggctctc tcattgccgg gctgctgtcg
     8041 gccaagggac agtatcaggt gcatctggcc gatctcacgc tggatgcgcc caaacgattg
     8101 atcgatgacc tggcgctgac gacggttact ccctgcgcgc tggatgtgcg gcggcctgat
     8161 tcggtcgttg cctatgtgac tgcccaccgt tttgacgcgg tgatctcctg tctcccgtat
     8221 ttttataatc cggcggtcgc tgaaattgcg cgagcccatc atctccacta ttttgacctc
     8281 accgaagacg tggccgtaac cggatgcgtc aagaggatca gcgaaggcgc tgatcacgcg
     8341 tttctccctc agtgcgggtt ggctcccggt tttatcagca tcgtcacgaa tgatctcatc
     8401 ggccatttcg aatccatcga ccatgtgaag atgcgcgtgg gcgcattgcc ggtccacccg
     8461 agtaatgcct tgaagtactc cctgacgtgg tcgaccgacg ggttgctcaa tgaatatgga
     8521 aatgtctgtg tcggaattga acggggggaa gaggttcacc tgcggccttt ggaagggtac
     8581 gagacgattg aattggatgg tctgctctat gaagccttca atacgtcggg tgggctgggc
     8641 actctcgcgg acacctatcg cggccgggtc cggacgatga actacaagac cctgcggtac
     8701 cccggccatt gcgaaaaaat tcaatttctc atgaacgatc tcaaactgaa tgaagaccgt
     8761 gagaccctca agcgtattct cgagcgggcg attccgcaga cgcatcagga tgtggtgttg
     8821 atgtacgccg cggtcacagg aacccggcag ggtgaattat ttgaagagac ctacgtgaag
     8881 aaagtctacc ctcggatgat gctcgggcgc acttggtcgg cgattcaggt gaccaccgcg
     8941 tcatccctct gttgtgtgct tgatctggtt ctggctcagc catccgccta tcgaggattc
     9001 atccggcagg aacatttccc cttgcgcgat gtggtgaaca atcggttcgg ggactgtttc
     9061 cgttcctagg gcaataatga cgacagaaga actctttaca cggttgggtt tggcccggga
     9121 acaatcgggt gggggcggtt cttcctcgac atggacacgt acgactccgg caggagtggt
     9181 ggaatcccgg aatccctcga cgggtcagac cctgggccga gtgtacggtt gcacggatga
     9241 tgagtacgat gccctggtga tcggcttgca gcacatgcag aaggtttggc ggacggtgcc
     9301 ggctcctaag cggggcgagg tcattcgtcg gattgctgag gcgctcagag aacagaagga
     9361 tgctctcggc agtctgattg ccctcgaagt ggggaagatt aaagccgagg gcgatggcga
     9421 agtgcaagag atgatcgaca tggcggattt cgccgtgggg ctttcgcgca tgttgtacgg
     9481 gtacaccatg cattcggagc ggccacagca ccggatgtac gaacaatggc atccgttggg
     9541 cgtcataggc gtgattacgg cgttcaattt tcccgtcgcg gtctgggctt ggaatgcatt
     9601 catcgcagcc gtgacaggaa atacggtgtt gtggaaaccc tcgcccaagg cgccgctctg
     9661 cgcagtggcc atccagcaac tctgcaatcg cgtcttggaa gacgccggat atccgggtgt
     9721 ctttgcactc tgtacgacag atcgtgttga actggctgag cgcctggttc gagatgaacg
     9781 gcttccgctg atctccttca ccggttctgt gccggtggga aggcaagtgg cggaggtggt
     9841 tgggcgtcgc ctgggccgca gtctgctgga attgagcggc aataatgcca tcatagtcga
     9901 tgagaccgcg gacctcgaat tggccacgag agcgattgtc ttcggcgcag tcggcactgc
     9961 cggacagcgc tgcactagta ccagacgagt catcgtgcag gattcccagt acgacaagct
    10021 ggtgtcgcgc ctcgtctctg cctatgggca ggtaaccatc ggggatccgc ttcaaccgaa
    10081 tgtcatgatg gggccgctga tcgatcgcga agccgtggcg cggtatcagt cggcactcga
    10141 ggacgtgaga caagctggtg gtgaggtgct ctgtggaggg cgcgtcttat ctcgtccggg
    10201 gtattacgtc gagccgacga ttgtgcgagc ccagaatcat tggaagattg ttcagcagga
    10261 aacgtttgcg ccgatcttgt atgtcatgac ctatcggacg ctcgatgagg cgatcgatat
    10321 gcagaatgcc gtgccgcaag gattatcgtc cgcgctgttt accactcacc ttcatcatag
    10381 cgaggccttc ctgtcggcct cgggcagcga ttgcggtatt gccaacatca acatcggtac
    10441 ctccggagcg gagatcggcg gggcattcgg gggagagaag cacacaggcg gcgggcgtga
    10501 agcgggatct gacgcgtgga aggcctatat gcggcgtcag accacgacga tcaactgggg
    10561 gactgaatta cagctggcgc aggggattac ctttgggcga tcggaaggag gaaaccatgg
    10621 ggcaagccgc tgagcttgat gccgtgattg ctcgtatgac ggcggatttc cttgcacaga
    10681 ataccgcggc cggaatgctt cgtcgcgcac tggacgacgt cggtgttgga tttgtgccgg
    10741 tcatggacca tttgacgatt cggaccatga acattgacca gcgcgcggag gaattcgtcg
    10801 cccttggcta cggatacgat gaaacgattt actacgacga ttggtatgcc aaggtcttca
    10861 gaaaagccgg atatccggca ctgtttgtcg accaggcgta cccggatgct cgcggacata
    10921 ccagcatcat tccccgttgg gtggataagt ttggtgatcg tgtgttccat cacgtagctg
    10981 tccgggtcga ggacattgag caagcgattc tgaaactgaa atcgaagggc gtggtctttg
    11041 ccggagaaat cgtcggtgcc cggggaggac cgcttcggca gatctttact gcgcccgaga
    11101 tgattgacgg gcaaccgttt tcggtcttgg aactcgccga acggcatcag ggctatcagg
    11161 gctttcttcc tccacaagcc gatagtctca tgcgctctac cgtgcgctag atactctgtg
    11221 ggttcccttc ctcatcgacg ctgccgcgcg catcttggcg gcgtctctct ttgcagaatg
    11281 gctaattctg cgtacaatgg ccagcgcgat atgagtagtt catcgcttcc aacacctgaa
    11341 atccatacag gaggtcatct ctatggtgcg aacggttttg ccgctctgct tcatccttgt
    11401 cctgttgtgt gtcgctacat tcgggattgg cgagagtctg gtgggtgcct caccttccgg
    11461 tcacgcgcct catgggaccg taaccatcga cggcatcacg gtacctgata tcggcccact
    11521 gccgactgca gttccgattc cctcttcaaa cgtcaattat gccgctaaga ttgagctggg
    11581 aaaacagttg tattttgacg gacgattatc caaaaacaac gcgatctcct gcgcgttctg
    11641 tcataacccg ggaacgggat ttgcggactc ccgtcagacg tcgatcggcg tcggtggtgg
    11701 aattggtggt cgccaatcgc cgaccgtcta caatacgggg tttaatcatg tgcaattctg
    11761 ggacggccga gcgcggtcgc tggaagaaca agcgatcggg ccgatccaca accccgtgga
    11821 aatggcggag acgcatgaac acgtcgttgc caagttgggg aagatcaagg gctatcaaca
    11881 agaattccgc gccgtcttcg ggacggaggt caatctgcag gggattgccg aggccattgc
    11941 tgcctatgag cgtacagtgc tctccacgaa ttccgccttt gataaatatg tgctcggtga
    12001 tgtaaaggcc atggatgaag ctgctgctcg agggttaggc ctctttaagg gaaaggcccg
    12061 gtgtattctg tgtcacaatg ggccgaactt taccgacaac cagtttcaca atctgggggt
    12121 tcctcaagtc gggccgatga aggaagacct gggacggtac aacgtcacca gggcagacaa
    12181 agacaaagga gcgttcaaaa cgccgactct ccgcagcgtt accgagactg ctccgtatat
    12241 gcacgatggc gccttcaaga ccttggagga agtcgtcgac tttttagatc agggaggcgg
    12301 cagcaatccc agactgagtc cgttgatgcg tccgctcggt ctcaccacag aggagaaagc
    12361 cgatctggtg atgttcctga aggcgttgac gggagagccg ataaagttca gcatgccgca
    12421 gttgccgaaa taaatcgcga atcctttcgg acagctgtga ctcttacggg cagagaggca
    12481 aaagggatcg gtctgtcgat aaaacccatc catgcaatcc accccaacca gaggaggcct
    12541 tctatgtttc gacaactttg cgtgcttggt gcgagcacgc tcgcgctcgc catgggcgga
    12601 ccagcacttg ccgcggatat gaagccgggt ccggccgacg gattcgacat tcatgtgatg
    12661 gccccgcata agatggagga cggatcggtg gccggtccct tccaccacta ttgcaaagcc
    12721 atcaagccag aggtcttgca gtgtttgttg ttcgaatcca cggcgccgaa tgccgtgtta
    12781 accgatgtgg aatacttcgt ggcgaaacct gtctctcggt cagcggtgcc gctggatgtg
    12841 tggaataaat tctatcacga tcatgaggtc gaaattgcca ccggtcgcgt gcaagtgctg
    12901 gacatgccgg atgcgcaggc gaaagaagtg gctgccgcgg cagccaaaac agatggcatt
    12961 atcttccact tatggccaaa aggtgcaaaa gctcctgatg gatcggtcgg tcatccgacg
    13021 tctgtggggc ataagccgcg gacggaataa catcgcgttt atgaacgatc gacgatctgt
    13081 cgacctatgg taaggaggcc attatgcgaa gtgcgattgc aatgatcgga ttatgcgcgg
    13141 ctgccctcac ggcgaccggc tgtgcggaaa cgtctcatga catgaagtcc acggctgctg
    13201 ccgccccggc cgcaacagcg gcgaaagcca tgccgacacc ggctcaaggg tataccattc
    13261 acgtgatggc cccccataag tttgaagacg gaacggtaca cggtccctat catcattact
    13321 gtaagcccat ctccccggag atcttacagt gcttgctatt tgaatccacg gactcgaatg
    13381 ccttgttgac ggacattgag tatttcgtgg ctaaatccgt ctcgcgggca catgtgccat
    13441 tggaaacctg gaataagtac tatcacgatc acgaagtgga gattgcaacg ggccgcgtcc
    13501 aaatcctgga tatgccggat gcgcaggcca aggaagtcgc tgcggtcgcg gctaagactg
    13561 acggcatcat tttccatctc tggccggatg gggcgaaggc gccgaacggg gaagtgggac
    13621 atccgcagtc ggttggccat aagcatcgga cggagtaagt aggttgtgtt tacgcaccgg
    13681 gcggaggggg agcgttcggc atcctctccg cccgtgttgt tttgtagaga ggaatagctg
    13741 tgatgaagtc cgtatccctc tttctttatg cgtggacggt agtcggcgta ggggccatag
    13801 cctggtccgc ggattccgag gtgttgcgtc ctcgcgtgcc ggtcgatcag atcgagtcgg
    13861 cgcgtgcagt gacgaatcca tttccggtga cgccggaaat gcgcgagaaa ggaaaggccc
    13921 tgtttgaggg aaaggccttt tgtcgcgcct gtcacggggc cgatgggaag ggcctgggtc
    13981 tggacctgga ctattctacc tttaagggac cgctgccacg aaatttcacg gacaagatgt
    14041 ggcaacaggc gcgcacggac ggcgaattgt tctggatact caaaaatggg agtcccggaa
    14101 cagatatggc atcgtttatt ccgttggttc tgaccgaaga agaagcctgg caagtgttga
    14161 tgtatgtgcg ttcattcggt gggagatgac agatcgtctc ggctcagtcg ggattgtgaa
    14221 gagtcgtcgt gggatgcggg aaccgggtaa cctgttctgt tgtgaaatat atacggatcg
    14281 tgagaatggc ccagagcaag gtttcgttgg aaaatcaacg acgataagcg gttaagaatt
    14341 gatccagacg gtgacgctct gaagcgggga gccgaagaaa ttcgatgccg aaatagtgcc
    14401 tgcggaccca gcggactgcg gccaggtcta tgaccaatgc atggtcgttg tccggcagga
    14461 ggagtcctac tgtcaggtag ctgccctcca tcaccgtccc ctcacattct agaaggcatc
    14521 catgtggtga gagattgaga acagtccctt ctccaatgaa tctggccccg gcgaacatga
    14581 tgggaaagac tatgcccagc cggtctcgag atcgttgaaa ggaaagggtg gtttgtgacg
    14641 gagaggggtg gagtcgtggc gttcgtggtg atgaaagatg tgactgcatg gagggcattg
    14701 tagtgcctgc aatggagttc ctcacatccc accttcgggg gatgtgccgg ttggttcttg
    14761 tgtaaggaat ttgccacgaa cgttgaggaa agaatcaatg gcggctggcg gcatagaaac
    14821 gatgactgca ggatcgacat cgccagggaa agcggcccag caaatgccat aagaaatcgc
    14881 gccaccctcg gcgtccagag cgacgtatta ctccgggaca cagagggcag caatgcgaga
    14941 aggcagtggg agtttcaacg gccataggtc ttactgtgaa gcgcgctctc agcgtagtgc
    15001 attgacggta aacaggtcga cgttatgctg ggcgcagtta cacattgtct ctgcctgcgc
    15061 tgcatggaaa aagtcatcac tcctgccacc acaccaaacg aatcacataa attcctgcga
    15121 tataagttgc tgcaatgata atcgcccaga tcatgatccc ctcctgattc tttcgcggca
    15181 ttctgccgca tccgtgatgc aacgatagga tgactagcaa ctcgcgtacc atcacgcgac
    15241 caacttgcgg ctgcgtgtga atgtcgaaat ttcttcgtgt tggcggttcg tgtcagcgaa
    15301 cagaggtttt cgtcgtcttg aattgagatg acgctgtatc caattcgata agagtgaatc
    15361 ggtttggata ccgggaaaag cccagatggc gaagccacta cttggcttgt tgtgatttga
    15421 tggaactggc gatcagccgc ggggaaggag cccgttcatg agggaggcgt cggcagggcc
    15481 gcggagtaaa gggacgtagc ttcctgcttc tgctcggtgg ttcgattggc gcatcagggt
    15541 tttgacgcgc gggaggaggc gttgcgagct cgttgctcga cggccaaggc agaggccgtg
    15601 atggcgtaat cgcactgtcc gcagtgcaaa aagcctccag tcgggaccaa actgtgatgt
    15661 cggcatcggg ggcagtcata aatcttcatt ggtgacctcc tgtgtcagtg caaggacgga
    15721 tggtgagggt atcgtgctga tccaaagggc aatgcggacc ctgcgcgtga tgtccccacg
    15781 atcaagaatc gcgtgcggtg atttcgccgg ggatcttcgt ggggtagcgg atatggaacg
    15841 ccttccgatc tccgcaattg acgcatcgcc accggccttc tggatcataa aaatcgtttg
    15901 atggttcgat tgccatatag cctccgcatc gggaacaggg catcgttcaa tctccttccg
    15961 cagcatttgt gggagccgat gaatggagag gatttacgga cggattgcgc cctctccatt
    16021 catcgggtcc atcactacgc tactcctccg gcctgatggg catggtcagc gccgtatcat
    16081 cttcacgcgc gggactttat caaccgttca gtcctgggtg cgctgcagat tgaagcaggg
    16141 cagctctcga tcatagtatc gcctgcgagc cgcgttagga atgtttactt tcctgaacga
    16201 ggaagacatt cagtgccagc atatgaggtc gatcgaggta atcgttctgc cagcacagga
    16261 gacgtttgtc tgtagagcga gcctactaag aacggaaagg cttctttcat cagttccgtc
    16321 gaagcttacg acaaccgttt cgtcgaaagg ggatagtctt gggagctggg aatttctgca
    16381 agaatagctc ggggaaagca tcggtggttg aaagcgctgt tgaaacgtac aatctgagat
    16441 tccgcagagt tgcttataga ttggggggcc tggcagaata cgtgcctaga tggccacgtc
    16501 tacatcgtct ctcacacaat gccctgatgt actcatacca ttaatggcca acattctggc
    16561 ctcgaggccg attatcgatc gcctgccaaa ggacaggcgg gtaaggcgag cacaagtccg
    16621 gccctctacc acgcattgta ttcatcaaca gagagtggcg ctgacgcgca ccgagtatga
    16681 cgagctcatg atcctgttca atagaactat ggggcaagcg tttacggtac cacttcagcc
    16741 gtctcagaaa atatgcacgg tgacctttgc cactccgcct ctcctcagcg aacgactggt
    16801 gtcgataagt cttcgaaaac tgacctccga cagggctaag tttaggctcg actaattttg
    16861 tgaaaatgct cggttgcgga gcttcttgcc tcttctcggt agagtggtat gccattccta
    16921 atggcttgtc atcggcagaa atgatacagc gattgaaatc gtaatcaggg caataataat
    16981 ccactcgagc acttccatcc gttgagtcgt cgcacggtcg gacatctttc cataaatgct
    17041 atccagcgtt tgtagttttc tgatgatgct cgcgtcccag gcctcaaggt gaaaccgctg
    17101 agaggccaag cgatagaccc tggccagata ctgatcgccc agcaacttca gggtattggc
    17161 cacacgttcg aagagcacgg cactgtccac ctgtagttgt ccgatttgtg taagagcagc
    17221 ctcatgggaa ccaggtagcc aagatgtaac tcgagccttt cgtgtgagcg caacataccc
    17281 ctgatccagt gcctgatcga gttgctcatc tagcgtacgc atttccagca gctctacatt
    17341 tacgaactcc aatactgccc gaacgtcgtc catatcttcc ccgaacacaa tggcagcatt
    17401 ccaatcgatg agagccgtgt cgtctcgact gaatgagata cgacaagatg tcgcttcgcg
    17461 aatctcctga tcggatagac gcgttcgttc gctgcgcagg acatgtgcaa acatcggttc
    17521 aaagggagtg cccaacgctt gaatggcaga tgggctgcaa tgctcgacgg aaaacaccag
    17581 gtagtcttct acctcgggcg agactgacgg acggtcaatg gccaatcgaa tgtcacgcac
    17641 caactggtcc aagcggtgac gcgagtcggc gagcagcctc tcattttcgt acaggctttc
    17701 gctcaagctc aggaggcctt cgaaaggtcc attgatagga atgcggtaaa tgacagtgac
    17761 ggcaccgaag tcatagagca tcacctccac ggcttcgctc gatcgatagt caccgaagtc
    17821 gagagacgcg gtctcctgaa tcaggcgcaa tggcgctgga cgatactcga aataatgtgg
    17881 agctcgtgct ttgtggcgca tgcggccgcg ttccttgatg gccgtcacat gtctctcagc
    17941 ttcgtcaaga ttgatggcca atccaatgtc ataggcgaac aaggcgtaac aggttccctg
    18001 ttcaatcacc aacgagggag catccgcgga gggcatgaga ctatctcctt tgatacaccg
    18061 atgatgcggc tatttgtgct cgtactgcaa gtcagccaat gttgaaggat gctgatctgc
    18121 ctgatgtgag gctcgccatc atgcgtccta cgtattacaa gaccgtaagg aagctgtaaa
    18181 ctctgtatca atcaggctca ggcgtattgc atggaaggat tggtatccgt atgaatgagc
    18241 aagcctatgc cgacaatcaa agcaatcatc ttcgatttcg atggcgtgct attcgacaca
    18301 gaggcgatcc actttgccgt attccgagag gtgctacggt cggagggaat cccgttgacg
    18361 ttaagtcgat accacgcagc ctacgtcggt ctctccgatg aggcttgttt ccgcgcaatc
    18421 ttgaatgacc atagaattgt ggtcccgaag gaggacatac aacaattagt tcgtcgcaaa
    18481 acggatctcg tgcaacgggc attccgtgca tctctgccgc gtgtatcggg cgtctcggaa
    18541 tttgtcagcg atgcatccat gcgttaccgg ttggccatcg cttcgggcgc acttcttcag
    18601 gaaattgtgt tgtgtttgaa gctagggagc atgcttacta tgttcgagca cattacggcg
    18661 gccggggatg ttcgggaagg caagcccaac ccggccccct atctgcatgc tctccacgcc
    18721 ttgcacggac gatcgccgat agcagccaat gaatgtctgg ttatcgaaga tacgccacat
    18781 ggagtggagg ctgcccacaa ggccggcatg agatgcgtcg cagtgaccac gacccttcct
    18841 gctgatcgac tcgcacaggc tgatctagtc gttccttcac ttcacgccct acgacttgag
    18901 gatctgactg tgcagcttcc ttgagagatt tccggtcttg ccgacagcag cacggtgtta
    18961 cgcaaagttt ccgacgacat gacggaatcg taacacacac tccctaagat acaatctccg
    19021 tcaggcatca gttcgagaga tggatcactc ggatcttctt gatgagtcga acgactgccc
    19081 tctccgcaaa ttcatcgccc tatgccgtcg aatcccgtca tgtaattgag cgggaggcgg
    19141 tggaattcct cgccaactct gtcacgttgc ctttttcctc aagcgatatc acggctggat
    19201 cagaaagcga gttgcaggcg gcggtcttgg gccgatcagc agacgtggac ctggcgcaaa
    19261 caattcacag atcaaactat ttgaagaata ttcgggatcg aacggagacg ggggagagtc
    19321 cgcgtatcgt catccaacag ctgcaggagt ttctcaacga ttcccaatcg accgtctggg
    19381 aaaactcctg ggttcggttt cctcgaaaca tgcttaataa ggaggccaat cagctgttgg
    19441 cgtgtgatct gcgagcaaat aggcgcgatc cgaaaggaac gcctcgtact gatattgacc
    19501 gttttttggt cacggaacaa ggagaggaat gggtcagagt gccggtcagc tatgttctgc
    19561 ggctcgcgct tgcccaagtc attggagcga tcgagtccga tgttcccatg ctggcgaaag
    19621 aagggcgtcg actgttggac cactttttga acgataacag ttcaccggag accttttcct
    19681 tctatgtgtc cgggatggat ccggcaagag ggatggggaa agccatcgcg catgaaacag
    19741 cgatccgctt tctcctcaca caattacttg ttcagtatgc aaatcgagca ttcggactgg
    19801 atgaaaccgg gcaacatgcg attgtctatg cttcgccaca cccgcctgtg cgtcagcgcc
    19861 gcctcaatgg actcatttcg gattcgttct atcgggaatt gtttgtgaat ccctgtttgt
    19921 ccggttggga tcagggcgaa gacaaatcgc gctatatggg actgtgccat gaagtattgt
    19981 ctcggagcca actcaatgcc gtcgtgcggt tgaaagacgc gggaattatt actcgcaacc
    20041 tcgtggtgtt gccggcaact tccaacatta gtctggccaa taacggtaca cacctcagtc
    20101 tgggtagccg catgttgacc gaaacactgc aagcgaatgg caagcgattt accgcggttc
    20161 atgaaaaggt cctcggcgat cttgtgatca aaatcgtcga acattttctg cccttgtttg
    20221 tagggaccta tagtgctgct ccgtatcgca tgaactttcg agactttcac cccgaaactg
    20281 cgttgggatt tttaccgcac gaactggact ttgctcacct gcgcatgctg tggcggcggt
    20341 ggaaaaagaa ggccggtctt tcggtatttg gccggtccat cacaccgcta gggccaccat
    20401 ggttggatcg agccatcagt tttatcttta atctccgtgg cgactgtctg ccagatttcc
    20461 ggttaatcga ctacctcgtg tgcctgttgt ctacggaaca aagcccggcg ctggacggca
    20521 tcccaggcaa cgatcttcga ctgaaacgag acctcggtca actgggggta ttcgacgagc
    20581 gcatgtcgct ctatctactg tacaagttgc gcgctcaatc agtaatgggg ttttcaggtt
    20641 ttgaaggccg acactacagc ctcttttgga accacacaga cgacttcgcg caagcggcaa
    20701 gcatgcaggc tctcgtgaca gccctggcgt ttcagtatat cgcaaggggt gaggtgagcc
    20761 atgaggatat ccccgacgat cccatgactg agagcgaacg ccgacaatac ctgttcgcga
    20821 cggcggttgg ggtagggaca gtgaatgtgc gagaggcagg ccccaatcga tttctcactc
    20881 ggattctgat gaagacccac aagacaagag cgagccggcg ttatgcgggg cacctgcgag
    20941 ttcgttgtga ggactaccgt atggcactgg tagctatgct cgaggaggat gcctcggacc
    21001 ttatcgagtc gtttcaaatg caggaatgta tgcgaagttt acgcgatcgg ctgtcgaacc
    21061 aggactccag tactgctggg aaactcacac acggcatcct gaactctgtg ggaagtccaa
    21121 gcgcctttga tctgtcgggc gaggagtttg ccctagcggc ggagggctac taccgtcgag
    21181 agttgagggc gcaatacctg cgcgaagcct gcgagtctgt acgagaggaa ttgtgcgcca
    21241 ttgatcaggg cgtttctgaa caggccacgc aatatcgcgg cctcgttacc gccatgatcg
    21301 gcacgcgttc ggcggtggac gcgtttacag aagagcagcc gcgcctgttg gcggagactg
    21361 ccgatgccaa gaccctccag cgttgtttgg ggctttgtat tatgagtatc gcccacgctc
    21421 ggatctctgc ggggcgaaca gaggagcagc cgctcgcttc atgagtatgc cgcatcagta
    21481 catcgaacgc gacagcaatc tggttgtgac tgaacggttt tgtggggatc aatggctcaa
    21541 ctgggcctac cgtgccttgt gggaaaatgc aaattggttc atgaaggcgc tcacttcacg
    21601 cagaaccacc caagccctcg gattctttaa ctatgatagg ccgttcactt ccacccaagc
    21661 agccgtggcc aggtttgctc gagctctgtc agtcgacctg tccgaatgcc tggatagctc
    21721 ggatcgcctc aacacaccgc gccgtctgtt ccagcggcgg ctgcggtact ggcaagtacg
    21781 gccaatggat gatgatccca gggccattgt ttctcctgca gatgcccgcc ttctgatcgg
    21841 ttccttcgcc gacgattcct cgatctttct taaaggaaag ttttttgatt acgacgaact
    21901 gctcggcaaa catcggccac aatggcgccg gctgttccag cgaggcgatt gggcaatttt
    21961 ccgtctgacg cccgataagt accattacaa tcactgtccc gtctcagggc gcgtcgttga
    22021 ttgttatgag attaacgggc gctatcattc atgtaacccc ggtgctgtgg tcgtcgcagc
    22081 aacgccctac tcgaagaaca gacgcatcgt caccataatc gatacggatg ttccgggagg
    22141 gactcgtgtg ggacaggtgg ccatgatcga agtggttgcc ctcatgattg gtcagattga
    22201 tcaatgctac tcctccgtcg cctacgacga tccacagacc atgcagattg gcatgatgct
    22261 caagaagggc tgtccaaaga gtttgtaccg cccaggaagc tcgaccgatg tattgttgtt
    22321 tgagcctggc cggctctcct tcagtgaaga cctgctacgg aatcgatttc gacctggggt
    22381 acagagccgg ttttcagaag ggtttggccg tccgctcatc gagacagatg ttaaggtgcg
    22441 ttcgacgatc gcatggcggt tagaaagggg gatcgatggg cgatagtacg tctgtcattg
    22501 cgcttctgat cgttttgtat gggttgctgt tctggtgggc ctttcgaacg ttgccacagg
    22561 agagctggca gtttgtggcc tctatgccgc gaatgaaact gtctgacggt cgctgggtag
    22621 ggaccaacct gacttactat ggggcctgga ccgcaactgc agtggtcttt gccgtggcga
    22681 tggcaatcgt cttaatgcaa agcgtcgcag tggcgcttga tgccattgct ctgttagcag
    22741 ttgtcttgct cggactatgc gtgccagcct caaaaattct ggctcgtctc atagaaggca
    22801 aagcgaatac gttcaccgtc ggcggcgctt ccatgctcgg cttgttctta gcgcccatga
    22861 ttatcgacgg aattaatctg gtgctgaact ggaccgggaa gggagcactt ccagccgctg
    22921 cggtcctggc agcgttgagt gtcgcctatg catttggcga agggacgggg aggctggcct
    22981 gcctcagctt tgggtgttgt tatggggttc ctctgcagga aagtggtcca cggctgtcgg
    23041 catggttcca gcagtatcac atcgcattcg taggcagaac gaagaaagct gcctatgagg
    23101 gtgatctgga agctgttcca ctcatcccca ttcaagcact gacagccgtc atgtcagtcg
    23161 gtgcaggcat caccggagtg tggctatttt tacaagggca catggccgct agcttcttga
    23221 gcactatcgc gatcacacaa ctctggcgag tagcatccga ataccttcgg gcggattttc
    23281 gtggcgttgg gacgctctcg tggtatcaaa tcctctcgct attaggcgtg ctctatgctt
    23341 tcgtcctcac attttggctg gataccttct cccccagcgt tccgaatatg aggttgggac
    23401 ttcgtgacat ttggagtccc gggatcattc tcgccctgga ggcgctgtgg cttggcatct
    23461 ttttcttcac cgggcggagc cgagtcactg catcgacgtt gaccatgcga gtgctgaggg
    23521 atcggatttg acaaggcgag tgccgcgtag ccccgtatac gtgttgttag cggtgctggt
    23581 ctgtctcacg tttcctcctc aggctagttc agaggaggaa actcttccag cggactatcc
    23641 ggcgtcgttg gaacgcgttc tctccgtcca gctgtcccgg ctctccacag aaggccccac
    23701 tctagatcga ttgctccagt tatcagagtt gtatatgaat attgcagatg atctgttcaa
    23761 tgatgaggag cgaaagcagc tggcgtacga agccgcatcg gcaatggcga agcgagccct
    23821 caccatagat gagcggagcg ctcatgctca ttttctgtat gcggcctcgc tgggtagcgg
    23881 ggaggcctca aagggctttc gaaaggtggg cttgtcttag gtgagctcaa gcagcatatc
    23941 cgacgcgcca tcgagctgaa tccgacccac gcacaggcgt tgcaaatgat gggaggcttg
    24001 tgcgctgagc taccctggct gctaggagga agtgataaag acgccgaatt gtacttaaag
    24061 cgggccattg cagcggatgg aaggtttacg aatgcccacc tcattttggc tcggttgctc
    24121 atgaggcaag gccgtttggc cgaggctcgt gaacatattg aatcggtcct gcatgcggaa
    24181 cacccgcatt atccctatgc ctggaaacat acatttcgac ccgaggcaga acggttactc
    24241 aatctgcttt caaaatagta agtaaaagat tgttcatgtc tcctgtcctg gcaaaagaga
    24301 tgttcaggct ccttcagcgg ggcagaacat aaggccagta tcttggatcg agataacgac
    24361 cactccgcgc ccccttccgc gaacctcctc aggtctctac gtcatttgcc caatagttaa
    24421 acgtgcccat acggcaggtt aataaaaccg taacagcgta cccctatact cgccagctca
    24481 cgtatctttt cccaacaagg gagacaattg catctatggg acctgcgctt cataccacca
    24541 ctgcagcaca tccattgacc gtccagcctt cgaggtctct tgcgtcgttc ggattctttg
    24601 ccttcgtgat cctgctcttt ctggggactc tctatacggt tgagcagcta tcctttgaac
    24661 tcatggcggt caagcaatca tccgcatggc catttatcct gctcggtgtt gccttagtca
    24721 ttgcattggg atttgagttc gtcaatggtt ttcacgacac ggccaatgca gtcgcgacag
    24781 tcatttatac gcactcgctg cctcctcatg cggctgtcat ttggtccgga ctctggaatt
    24841 ttatcggggt catgatggct tcgggcgctg tcgcgtttgg tatcgtatcg ttgctccctg
    24901 ttgagttaat cctccaagtc agtgcaggcg ccggattcgc catggtgttt gcgctattga
    24961 ttgctgccat tatttggaat ctgggtacct ggtatatcgg ccttccctct tcaagttctc
    25021 acactctcat tggctcggtg atcggggtgg ggctcatgaa tcaattgatg gccgggagcg
    25081 cagaggctac gagtggagtg gactgggccc aagcgttgaa cattggaaaa tccctcttag
    25141 tttccctgct gtttggtttt tccgtggccg cattcttatt acttgtggca atgcgtgcca
    25201 ttccaatcgc aagtctctac cgtgcccctc agggcaagac tcctcccccc ttgtggatac
    25261 gtggcatttt gattcttacg tgcaccggag tgagtttcgc tcatggctca aatgatgggc
    25321 aaaaggggat gggattgatc atgctcattt tgattgggac ggttcctacc gtgtatgcgc
    25381 tcaatcggac tgtggacatt tcctataccc cggccttcgt agaaacatcg agacaagcgg
    25441 aacggacatt ggacaggtat acacatggac agccagcacc aaccgaccca cggcatgaag
    25501 tgggacagta cttgaggtct cgtcaactca tgccagagac cattccggct ctacgggccc
    25561 ttagtgcaga ggtgcgcgaa gggatttcag cctttggatc agtggcacag gtcccggatg
    25621 aacaaaccgg caatctgcgg aacgatatgc atctcatcac ggaagcgctt cgtcgcatgg
    25681 agaagcagca tgatcctccg ttgtctgcgc cagacctaaa gatcttgaaa gagtatcgct
    25741 ccactttaga tacagcgaca aaatttatcc ccacatgggt taaggctgcg gtggcattag
    25801 cgctggggct gggtacgatg atcggatgga gacggatcgt ggtcacagtt ggcgagaaaa
    25861 tcggtaaggc ccatcttacc tacggacagg gcgcatgcgc cgaaatggtc gcgatgggca
    25921 caattggtgc cgcggacatg cttggtttgc ctgtgagtac tacgcaagtc ctctcttcag
    25981 gtgtggctgg aacgatggcg gcgaatcaat cgggactaca gtggaatacc gtgcgcaatc
    26041 tcctaatggc ttgggttctt acattgcccg cttctatcgt gttgtcgggg agtctctttt
    26101 ggatttttag gcacctcgtc tagtcacggg atgctaagtg gggcttggga agatgttgag
    26161 cgcatttgtc ttggaagagg gtcgagtcat ccaggctcag attgacgaca aagacgattt
    26221 gactcgcaag gacttggttt gggtggattt ggcatacccg agtgaagagg agcgcgagtt
    26281 ggtgcagtct gtgttcccac tcgaattgcc tgatgatgag gagttacaag acctcgaggc
    26341 gagcgcacgc tattaccaag atcagcacgg catacacatt cgatcaacgt ttgtccaaac
    26401 cgctgacgat gtggcttcaa tcgtgaacat gtccttcagt ctgcatcagg gccgattgtt
    26461 gacagtccac gacgatgacc tgagtttact acgactcttt cggatgcagg cgcgatcgag
    26521 taggggaatg gtgcgtgacg ccgcagacat cttgttgagt tgttacgaac tcggggtgga
    26581 gcgagatgcg gatgttcttg aacgaatcta tacggctttg gatgaagtag ggacagttgt
    26641 gttgagccgg aaaaaagaga tcacctctcg aatcatgcgc gataacgtcg agcgcatcgc
    26701 cgcgcaggag gatctcaatt caaaagtacg attggatttg atcgataaca gaagggcgct
    26761 ctcattcctg cttcgaagcc gtgtgctttc acccgaccaa gcccagcgag tcaaaggggt
    26821 gttgcgtgat atcgattcgt taaatgggca cacaggattc attttcgaca aaataaactt
    26881 tttgatggat gcgctgttag gcatgatcaa tctcgagcaa aacaaaatca tcaaaatttt
    26941 ctctattgct gcagtggtct ttctgcctcc cacccttgtg gcaagcatct acggaatgaa
    27001 ttttgagcat atgccggaaa tgaaatggcc tttgggctat ccgatggcgc ttcttttgat
    27061 ggtctttgcc ggcggcgcgc catatctgta ctttaggcaa aagggatggt tggactgaac
    27121 agcatgtacc cacgctcatt cgaggaaaga ggagaacgat gaattcccag cagaacaaca
    27181 aacccagtcg cccaggacga aaaggcgcac tcgcaaaact atcagaaccg caagtgggag
    27241 atgctccacc aaagctaaaa tcaaagccaa gcaaacgccc tatgctgcac gatgcctcga
    27301 cagtacctcc tcagaagcac gtgctgacac aagaagagtt tagagcacga gtgtcacgca
    27361 aagcctttga gctctatgaa attcgccgat cggttactga agtggatgat tggattgcgg
    27421 ccgagcgtct ggtgaaagtt gaattactca gtgaagaacg aggggcagga tcagtctaga
    27481 ttttacattc tgatcattat tctgctcatt atgagtggat cggccattgg gattgcatat
    27541 cggcgtgagc agattgattt gtgcgtatcc tgccgtattc aggtgatcgc ggaacgaggc
    27601 aaattgcgaa ggttatgaaa aggaagatca aacaacgatt taatcgactt gaaaagagcg
    27661 tccaacagct cgttcaagat attcaggctc ttaaattctc gttcagcacc attgaagctg
    27721 agaaaaggat ctacgcttcc gagaaagcca cgcgggcaaa taaagtggca acaacaagac
    27781 ggggggcatc tccaagagcc atgaagataa aagagccagt agcgacgaaa agggccgctt
    27841 ccaaatcacc ctcgaacctt ttggcaagtt agtcgaagat agcccaatct ccctaagagg
    27901 acgtaacatt cggactgttg ctcaactctc cgaaggagcc aatggtaatt ggcacataca
    27961 tgcctccggc atatatggaa cattgactaa tcaagaattg ttgatatagc atggagacat
    28021 gcgcacgaag accaaacagt tgaccgacgt ggccacggta tttcacgcct tatcggatga
    28081 aactcggctg ctgatcctcg aacaactaaa gggcggcgag cagtgtgtgt gtgatctgac
    28141 agagattttt aagacgggac agtcacgact ttcttttcat ctgcgagtgc tgaaggaggc
    28201 agggttaatc ctggcccgtc ctgaaggtcg atgggtgtat tactccctca attccgatgc
    28261 agttgaagag ttgcaggatg tggtgaattc gctcaaagcg gtgtcatccc gcgccgcgag
    28321 gcagggctgt tgcccgtgac atttttttgc cctcatacat caacaattat tgattttgcg
    28381 ggatgaaact tatggatgcc gccggagtca tcgctcaaca aactcttcct acagagaaga
    28441 gactcaatgt gttcgaacgt tatctcactg tctgggtggc cttttgtatg gtggcaggta
    28501 tgttgctggg aacgtccgct cccggcctga tccagatgtt gcgagggatg gagttcggcc
    28561 aggatagcca cgtgaatttc cccatggccg tcctgatctg gctgatgatc accccgatga
    28621 tgatgaacgt agattttcgc gctgtggcga aagttggtca gcgccctcag ggcctattgg
    28681 ttaccctatt tgtcaattgg gtcgtgaaac cattctctat ggccctcttc gcctggatct
    28741 tttttcgttt cctgttctcc gcctggatga gtcctgctga agccgatcaa tatattgctg
    28801 gcgccatcat tcttgctgcc gcgccatgca cagccatggt ctttgtgtgg agctatctga
    28861 cggatggtga tcctgcctat accctggtcc aggtgtcggt gaatgatttg attatgttgg
    28921 tgttgttcgc tccaatcgtt ggcctgctcg tcagtggagc ctcttcgctt actgtgccgt
    28981 tggcagttct tgtctattca gtcatcgggt ttattgtgat tcccttggtg ttgggagtcg
    29041 tgatccgaca ctggtttatc agtcagcgag gaaagacgtg gttcgaggag acgctcctgc
    29101 cgagctttgc gcccatgacc attggcgcgt tgctggtgac cttagttctc atcttcgcct
    29161 ttcaagcgga caatattctc gccaaaccac tgcatgtctt gctgattgcg atcccgattc
    29221 tgctgcaagt gtacttcaat gcgtctttgg cctacgggtt gatgaagtgg gtgaaagtcc
    29281 cgtatgccgt cgccgccccg ggagcgttaa tcggtgcgag taacttcttt gagctcgctg
    29341 tggccacggc cattgcgtta ttcggcccag aatcgggagc agccttggca accgtcgtgg
    29401 gcgtcctggt cgaagtccct gtcatgctgt cagtgtgtca tgtgtgtaac cgtacccggc
    29461 attggtttcc ttcgaaggag gccacagcat gatgcgtatc agtgacggaa catctccagg
    29521 tgctgttcga attgtcctgt ctgcactcga atcacatgct gaaagacgac caacacatct
    29581 tcatgactcg gactctgatc aggcaaaccg gactgctaaa tgtagggcca cagaaagtgg
    29641 ggacctacat ggataggctt ggtctgtttg ggatcctggc cgccaccata ttgataggtg
    29701 tgtattgctt cgaaaaagcg ttgaagccgt tgtggccagg actaagccag gcccccaacc
    29761 ttcacccaca ttctccaaga gtggttgctc tatatggctc tgttcatagg tctcctaccc
    29821 ctattgtacg gaatgctgag gctcgcatgc tcagtgagtt gttcgcaggg ccaattacaa
    29881 actgaattgc caagagggag gctgcatcat gaaacgattt catctccaca tgggcgtaga
    29941 ggatctcgac acgtccattc gcttctacac ggcattgttc ggggttcccc ccgtcaaaac
    30001 taaatcagat tacgccaaat ggatgctaga tgacccgcgt atcaatttcg cgatctccac
    30061 gcgagccaag aaaggtgtcg accatctggg cattcaggcc gatggcgagg acgaactcgc
    30121 agcgctacgt ggccggattc aggccggcca cttgccggtc gtgaatgaag ggaaaacggt
    30181 gtgctgctat gcgctgtctg ataaatcgtg gatccaggat ccgtccggcc taccgtggga
    30241 aacgtatcga acgatggagg atgctcaact gttttcaggt tccaccgatt cgtcggaagg
    30301 cgcctgttgc acgcctctga ccatggcccc gacgaactgt tgtgaaccat ctgaaaaaac
    30361 ggcggggtgt tgcgactaat ggaaagcgca attgtcacag tattggtgct gtgcacgggc
    30421 aactcctgcc gttctatcat ggcggaagcc ttgatcaatg ctctccgccc cgggcgctac
    30481 catgccgtaa gcgcaggcag ccatcccaca ggatatgtgc acccgaaatc catcgacacc
    30541 ctgaagcggc atgggattga cccgggtcag ccgaaaagca aatcgtggaa tgagttttct
    30601 catcaaacgt tcgatctcgt gattacagtg tgtgatcagg cggcggccga agcgtgcccg
    30661 ttatttctcg gagcaccgac caaaatgcat tggagtacac cggatcctgc gaaggcgact
    30721 ggatcagagg tcgagattga tgcggcgttc gaccaggcgt ttctgatgtt aaagaagcgt
    30781 gtcgaacatt taacctccac aacttgagcc atggcgattc ctgacaagac cttcccactc
    30841 agtgagcggt ctggcaggaa tgtctgcatg aacagttggt atgggcaatc gtggagagtc
    30901 tggcggctga gagcgaatcg gagaggaggc ggatttgaag tgaatccgcg cattcctatc
    30961 cgtcaatgtc gccgatacgc ttgaaggcgt cgagcaagca gcctaaagaa cgtgactctg
    31021 ccctggcagg gagcgcggtt gcgtttcatc ccatagaggc ctcaatgcac cacccattca
    31081 ttgagacctt gaatctcgag tgtgcgaatt cgcgacgagc gctgattttg gaagatgagt
    31141 gacgagtctt gtcactggtg ccgttgtgta tcgactccac ttcgtttgcc ggagcgtcac
    31201 tggttcacgt gtacgacctt ggccggcggg aaaccgttga agtaggtgct gctggcgccg
    31261 caataggccc ccatattctc ggcatacacc aattcgccgc gatccatgtc ggggagctgc
    31321 tcgaccaaac taatggtatc aagcgcgtca catgttgggc cgaagacggc acagatctgc
    31381 gtgggatctt ttctgaagct tttcaagtga tactggcagt ggtcgaagat cacgccgctg
    31441 taggtatgat agaccccgtc atcgagatag tagcagagct tgcctccacg cacggccttg
    31501 ccgatgattt gtgcgacgga ggtggcggcc gaagcgacga ggaagcgacc tggttcggcg
    31561 agaatttcaa ttggctgggg aaacaggcgg tccagttcgg cgttgatggt ctttgccaag
    31621 ctctgaaacg cgggcacgga gtcgtcgtaa tgggcgggga agcctccacc gatgtcgagt
    31681 agcttgaggt caaatccgcg agtcttggcc tcggagaaga taccggcagc gaggtgcaga
    31741 gcctggatgt agttttgcac attcgtgcat tggctgccga cgtggaagct caggccctcg
    31801 accacgagtt tattattgtg cgcgaaggcg atgaggtcca ccgcttcgcc cggcaaggcg
    31861 ccaaatttgc tcgataactc taccatcgag cccgtattgg gcacactcaa acgcagcacg
    31921 aggcctgaat gcggagcgta gcgggcgatc ttgagcacct cttcgtgatt gtcataggtg
    31981 atcaatggct tgtactgatc taactgcgtg agcgtttcga tggccttgat gggattggca
    32041 tagatgatac gatcccagat gaaatcctgg cgctgctgag ccggcaggtg tttgatcttt
    32101 tcgtgcacga tcaagaactc agccatgctc gcgacgtcaa agctggctcc cacgtcataa
    32161 aacgtctgca cgatggccgg atcgctgttc gctttcaccg catagtagac ttgtacgcgt
    32221 ggcaaatgtt tgcggaactg gtcatagttc tctcgcagca cggcatgatc cactacgacc
    32281 aatggcgtgc catgcaggtt tgccaggtca agtaaggttt tttgatccat gatccctctt
    32341 catttccgat gtgtcgcgac acccccaccg tatctgccgt tgatgactca aagtcgcatg
    32401 gccgggctta atccccttcc atgatcagaa aattcttaaa ctgccacggg tcggacctgt
    32461 cgagctgggg ctgattgtag cccctaaaaa agttggaata gtgcttcagt ggattccagt
    32521 ccgaggcagc ggagatgaat ttccccaaat agggcttgct catattgagg atatagtcgt
    32581 gcgggaggtt gtcgggaacg cacaagcccc tcgaaggatt ctcaagcatc cacatacagg
    32641 cggcgacgac ggaaatagcg acctgcatgg tggtcgcatt ctgatgcggc accaagcgac
    32701 gggcctcttc aatgtctaga tgggagccgc accaccacga ctggtacggg tgccccatca
    32761 cgagtacccc caggatgtcg gccccgctga tgatctcgtc ggtcatgata cgggttcgcg
    32821 attgcagtct gtagtcggag ccgcggagtt catggagaga cgcgatggcg gcatcgcagg
    32881 ggcaataggc gtagtgcacc gttggccggt aaatggcctt gtcgccttcc cacacggtga
    32941 gcttctcggt aatgctgaac gcctcgccgt ggcggacgac catgccgata atgttgtggt
    33001 ctggcggcac ccaacttgcg acgaaggtgt tcatgcccat cctcgccagg cagatttggc
    33061 ttttggggcc gtcggggtgc tcgtaggcga agcgaggtaa ctgtttttcg tgggttcccc
    33121 agcccatttc ggccgtggtc gtgccttcct ctcgaaaacc ttcgacgctc caggtgttga
    33181 cgaactcgtc gacctgcttc ggccgatcag tgatttgcgt atcgcgctca ctgcaatgaa
    33241 tgactcggac cccgagctgg tgggcgagat ggttaaactc ctgcgcctgg gcatgatgcg
    33301 cgattctctc tgcctgctcc ccactgaatt tcttatctgc cagggtctgc cgggctatgt
    33361 ccagaagcgc atgcttggtg aagtgactaa tcagtccggg gtttgcccca tgttcgacga
    33421 ccgccgtggg cccaggctcg ccccaactgg ccatcatgcg acggagattc atatggcgcc
    33481 agtacaatgt ccgctcagta ggatgtttgt cgtctactcc gcaatacgga tcccaaatct
    33541 ctactgatgt gttgatgtac agcacacctc tgtcgtgaca ccactgtacg atttcgcagc
    33601 aatcgatatt ccaagccaag tcgatgagaa ggtcccctcg agacgcgtgt ttgcctagta
    33661 gcgtgcccag attctctggc gtcacgcgat ctttgacaaa cgtcacacct tgtttgatcc
    33721 aaggggccaa cgctgcctca ttgggatcaa agtccaagat agtaatgcgt ttcggatcga
    33781 cgcctatgtg tttcagcaaa atgggcaatg tgcatcgcgc gacagcccca aacccgacga
    33841 acaaaatgcg atttggaaat acctgcatct aaagcctccc acttacacaa cccgtaggtc
    33901 tcgaaccctc cgcccggtgg ccaacaatac tcggctggga tgatcgtgcc acccgtattc
    33961 aggaggggtg gtattgtagt gcactgaagg gcatttgtgc tctgtctggt gttaagaaac
    34021 ggaaaatttt attctctttg tattgcgagt cttcatcaca tgccatcgat atttcagggc
    34081 ccgggagatc ggatcggcag cgagaccggg aggcagggag gatgtgacga ggctcgcgaa
    34141 acgaggaaaa gtgtgggggc gtatttgaac actaggagcg gaagggaaag gaacttgtcg
    34201 cgtgcgccgg atgcgattat ctccaggatc acagtgcaaa tggtcgaagg cgagcatttc
    34261 agccgagccg ctgttgtatt acctatgttc gtttgaacat tcgttgtggg gttctgaagt
    34321 gctatagagc cagagttcga ggctccttcg ggaacatgca agttcgtata gattgccctc
    34381 aaggtgatcg gggcttgaaa tagcgtcaac gctatggaga gtttgcggga tgaagtggtg
    34441 atcctagatt gccttggaac cagcgccaca gccggcaggt cttgaagaag gtgtgaatta
    34501 cctgagtgct tgaaaaaatg gtgggccgtg taggggtcga acctacggcc cgctgattaa
    34561 gag
//
LOCUS       DAOQEW010000016        34164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_267_length_34164_cov_8.453575, whole genome shotgun sequence.
ACCESSION   DAOQEW010000016 DAOQEW010000000
VERSION     DAOQEW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 34164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34164
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_267_length_34164_cov_8.453575"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..723)
                     /locus_tag="PLS35_03280"
     CDS             complement(94..723)
                     /locus_tag="PLS35_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNI17706.1"
                     /translation="MLTAIGDVMRRVYERGWITTRDGNISMRKRDGKYLYITPSGWRK
                     TIVHPEHVVRLEVVTDPTSGHLVPSVREGQQPSGELWMHWNLQRDTKKTRTVVHVHAT
                     HIVAAMYAGIDLQAMSAEFPEISRYTRVGRTVPALPALSRDLADVTAECLGLRKDGTL
                     EFDIVGQKNHGVCAVATDPWAAYEHIERLDHICEIVLKSGVAARSSGKL"
     gene            865..1848
                     /locus_tag="PLS35_03285"
     CDS             865..1848
                     /locus_tag="PLS35_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI17707.1"
                     /translation="MTILNGFTPALLLRNPHLMTLIPRYWPRLRWKENLPCCERLFTT
                     QPGTQLLGFCHWQDDPTTAPTVLLVHGLEGSADSHYMRGMAAKAFCAGFNIIRMNQRT
                     CGGTDHLTPTLYNSGLSNDYLAIIRELRERDGLSHIWLVGYSMGGNLVLKAAGELQRS
                     EAALAGVVAVSPNIDPTQCVAALEEPRNWLYHAHFLSSLKARMQRKAALFPGQWNLSP
                     LRLMKTITQFDDTYTARDGGYRDATDYYNRAGARHVLSEIAVPTLIITAQDDPFIPAS
                     MFDSPGIKNNRYITLAMLRNGGHCGFFQKRRYGEDRFWAENRIIEWLSSRA"
     gene            complement(1950..4958)
                     /locus_tag="PLS35_03290"
     CDS             complement(1950..4958)
                     /locus_tag="PLS35_03290"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI17708.1"
                     /translation="MKVLAISALVNALAATGLGMFVYFRDPAAARNRIYGLYCLSIAF
                     WSVFYCGWQLAESKDLALSLLRLVMAGASLIPILYFHHTVTFLDIAVRHAKALKIGYG
                     LAGLFLLVDTTPWLVAGVHPAMSFSFWPVPGPFFHPFLAYFLWYVVYATSLVGVALRD
                     ANGIRRHQYAYMLAASVIGYAGGATNFPLWYGVPLLPYGTVLITMYTALMAYTIVRYR
                     LMNISVVLNKGLGYAIVLAIIVVATSIGAVLSNRATGHSTPPLLAGTLFLICALWVLS
                     NNPRSSSNTVFSAVCGAACLWLFGCFMLFSATHEEEALFWVRVIYTGVVFLPALTYHF
                     AQRLAGGAVPDRAIGWNYALGGLFWSLLFTTYVVDGQYVYYWGRYPKAGVLHPWLVAY
                     VVGGGGLTVYRLYQGYRFHQTSAPLLGAQLKYTFVALLLGFSASLDFLQNYGIGFYPV
                     GYLLAGLSVTVVAYAIARYELMDISFVPSRPKVMLSIKLAGLIPAYMMILLVIRIFTG
                     TFHYLLAGVLFGLFVIVSSGLANLQKGVERAIGQTLFRERYDAYDTLVQFSKSLVAIL
                     ELKSLTKEIVQTLARVMNIKTASVYVLDKEQGVYSLTASYGFVTRDVIVPPIKMESEF
                     PRALLRTETALVREEIEYDKADVDYLRLLDTLKGLESEVCIPLVSKERLVGFCNLGRR
                     ANHGMYSTEELALLKTLAQHAAIAIDNALLYEDLRRSQLLMRRTDRLRSLETMAGGFA
                     HEIRNPLTSIKTFVQLAPDRRDDVEFMEQFSQVVCEDVERIERLVHEILDYARYMTPK
                     LTQESLNDVVSSCLYFIEVKASSKAITIQKELAPDLPYVKLDRQQIKQVLLNLFINAM
                     EAIGGEQGGRLSVRTRKLVKSVNEPWVQIEVEDSGPGIEPHDLDHIFDPFYTTKHESG
                     EREGTGLGLTIAHQIVQEHGGYVEVFSEVGHGTKFMVSLPVNPPMAEWQAAQPVYDDG
                     RKLAGSFLARPHLGLEQQFSKPDSPLKTRT"
     gene            complement(4992..7724)
                     /locus_tag="PLS35_03295"
     CDS             complement(4992..7724)
                     /locus_tag="PLS35_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI17709.1"
                     /translation="MSSDSTARNVTQPLSALCGHEDSLNVPAVERKKTQDAWSLSLAE
                     RRASRRLALRLSTQLFFQGHTWKGVTRSLSLGGLSMDFVSDIPAMLNQRMMLSFSPDE
                     RGLDSVGIVCGIRSSEDVHVPGRTQAAVTLAIQFIHLSDADERVVASLLSHGATAPRH
                     MRLAAALVVQEGEESLIDTGSLQTAATPPASMQLVRGPERPREERRRQQRVVVGLSTE
                     VSLRTRGGDRPLSAGLTTDLSPNGACVHLSAEEDLLGSELELRWLPSAPLQGSSTDSL
                     SATLCSVVGEVVWTRPVAPEARPGCVPVAGRTVLAGVRFLPVAKEAEDIIEHFLGQVS
                     MPSPETKVGSPTVLSEFSECFRPSGLRIVICHDRPQVAPAADAPIIVLAPGYGESKRD
                     YVPLAYYLAGNGFHVVRYDNVNHVGESDGLVTQFRLEDMEADLETVLDHVANQWPGRP
                     IGLVATSLAGRVALKVAGKVSHVRLLTLINGIMDVRHTLQAVHQEDLIGEHLSGVHKG
                     VVNIMGLTIDADRWLAHAVEGEYADLLTTQRDASRLQTPVVLFHAEQDAWVDPASIER
                     VTEAIGPYLRHSFVVPGALHRLQESPRKARTVYRQIAVCCQQELWPARRPERMVEPSH
                     REIGAQNRLERERSKKRKPMGKSDHVAFWQEYLQNFQTIPNVADFWRLMDHLYRLMGD
                     CHKGERILDAGCGNGNFGVFLQLNQAFRQRYGKRGDFRSPDYVGLDFVPAALAQAMSN
                     FEQVGATLQGQFYEGLRAYEPMSMRVCQADLESALPFPDHSFDRVVCNLVLGYVRDPL
                     FTLREYLRVLTPQGRLVISNLKPYADLSAIYRNFVDTAQTKDQVEEGRRLLDNSGRIK
                     EREGEGTFHFHYQTELESLLRAAGVSRPRVYSTFGNQALIAVAEKGMAHVTVAA"
     gene            8344..8784
                     /locus_tag="PLS35_03300"
     CDS             8344..8784
                     /locus_tag="PLS35_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17710.1"
                     /translation="MLPTGPGSLALSLIMQTAYVRLYIGLMNILLTGFVLGCQTSPPV
                     TEGYPHQQALQAKTLQEVLACAGNPLQKREDGPVTMLRYYREAPLLEESMVSSKGSRP
                     TVHHGCWATVILKDQRVDRVRYHFVPSSVDASNDCEEIFANCQE"
     gene            8781..9701
                     /locus_tag="PLS35_03305"
     CDS             8781..9701
                     /locus_tag="PLS35_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17711.1"
                     /translation="MKTRRFNGRKILGLLLMAWCLQSVADWPPPAYGLEKYGRPLPSM
                     DEPEHEAREEEETLFGGYFLTGAFVKNPTFAARPDNTGLVGMRHMLHLETDLYKQYLT
                     FYTDQNFFSDRTDGWIKLSEWDGTFAFTGVLDRFNWRLQYERDAPLDRRGLKQAYADA
                     LVTARFQAIQDSTWWRRTFPHQNFTAYAGAGWLFHNSQYFARPNNTGRALFRYVAHAD
                     LDVYKNKVVLYGDVNLFTDREASQTLNPSELDWIVGIAVRWQDMELAFYREEDRPLDQ
                     SGLVQKYFAVQLRFAFDVSKGAMERVGWKR"
     gene            complement(9709..11199)
                     /locus_tag="PLS35_03310"
     CDS             complement(9709..11199)
                     /locus_tag="PLS35_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNI17712.1"
                     /translation="MLNRIFGIETEYGLLVNQDRPDHSPSWVAQRIRDHIFHIARRGV
                     LDLHHRGHDEPPGNGGFLTNAGRIYIDMGHLEYASPECTTLADLVASDRAGDRIIQQA
                     VTALGLDETVSLIKNNIDHETDATFGSHENYLVTRQFPFSRRGLGPLVTFLVTRQVFT
                     GAGRIGCAGDPNEWVQIGGLILHRPGLRDAQDRSIVPFQISQRADYIVNDFFEWVQHN
                     RAIVNTRDEPLADPNQYRRIHLLCGDSNMAEYATALKMGTTGLVLQLIEAGQAPRGLS
                     ISEPVEALQDISRDPDRQWIVRLESGQSISALDIQEQFLAAAQRHCKGQDEETDWVLE
                     QWESVLTDLRHGYEKLVGRIDWASKLWLLETYREAEQLAWDDPMLKSLDLEYHNLHPE
                     RGLCYGLEEEGRGPRLTTDKGVQLAQDHPPRNTRAFGRGELVRHLLASTASGAGADAA
                     HSFGDHASCEYIINWSNFKLRDTEPFFMADPFKTYVQDVRSHLSAR"
     gene            complement(11279..12103)
                     /locus_tag="PLS35_03315"
     CDS             complement(11279..12103)
                     /locus_tag="PLS35_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="20S proteasome subunit A/B"
                     /protein_id="HNI17713.1"
                     /translation="MYEEPYRWIEAVGNRRQYLDEQFAMGAPVVALAYADGIVLLTVS
                     RGTPKLYEIYDRIALGGMGHPADLEKLRFSLLEMAHVEGFNRSPSDVTGARLMKYGLA
                     PAIKQAFEEIYKAPFIAKILLAELGVKPERDLFLTINYDGNFEERRGWAALGATSAVQ
                     VQMGRYIDAQPGFAQASLAQAVETGLCAWAVGSLAQTSSGTSDGDSSVRDAAEKPDAE
                     ALPDKTALLAHVRQTVAEKSLECVLLERNGPGSSKYRTLRPDELGRVLPSAVRASQ"
     gene            complement(12173..12946)
                     /locus_tag="PLS35_03320"
     CDS             complement(12173..12946)
                     /locus_tag="PLS35_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HNI17714.1"
                     /translation="MGMQGDFFQLLKEQGYQFGNPSAAAAGMDIPNATTILALKYRDG
                     VLVAGDRRATAGNMVMYDRTDKVLEIDRHSVLAIAGVPATAYEMVRVLEHSFKYYRRT
                     QLQELSFEGKLRAVSKLLKENVPAALAGTGAVVPVFAGYDHEQEAAKIYFYDILGAEF
                     EAVEYAVSGSGSPTIRGILHYVNTWGPQPLAAMPEEQATVQALRLLSSAAEFDSATGG
                     VNRELNLYPVVKLITAAGVQTIPDTHLKHLYERDVVRAL"
     gene            complement(13002..13199)
                     /locus_tag="PLS35_03325"
     CDS             complement(13002..13199)
                     /locus_tag="PLS35_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HNI17715.1"
                     /translation="MMRYTMMPERREAPGDPMPKPTGPSEEGGGPRRPDTGSPDKDNL
                     LKRMRKVDPKQAERYRQRTGQ"
     gene            complement(13196..14719)
                     /locus_tag="PLS35_03330"
     CDS             complement(13196..14719)
                     /locus_tag="PLS35_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HNI17716.1"
                     /translation="MHLFGIETEYGITREDLDAVDPVEESMELVRAHLTGEFERRWDY
                     HGEDPHEDARGFRVSGLQQDKEEDDFAKVDAHRPYSFHEMKSDLVLPNGARFYNDHTH
                     PEYSTPECRTLKDLLAHDRAGERIVQRAADRRNQRLGGAHVQLYKNNTDFHGHSYGCH
                     DNYLVPRALPFPQLVQGLLPFLVSRQVIAGAGKVGMEAQESGFVPGPFQLSQRADFME
                     AELGVDTMHNRPILNTRDEPHADRAKYRRLHLIVGDANFCEYATALKVGTTRLVLDLL
                     QRGAAPLVELEQPVTAIKQLSRDPDLKTTVRLKDGRTISGLAIQQEYWNAASRVCGGS
                     DPDADWVLREWHDTLQCLGGDRTPLVGKLDWVTKQWLLETFMREEQLAWEDPWLASLD
                     LEYHNLNPDRGLFLGLEAEGKTWRMTTERDIAQALVAGPSDTRGGLRGLCVRRFPEQI
                     TGMQWERVQFAGGLRGRTLEMGDLFDPEAVQACAGLFETAASPADALAAWAKRKDAQ"
     gene            complement(14757..16508)
                     /locus_tag="PLS35_03335"
     CDS             complement(14757..16508)
                     /locus_tag="PLS35_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI17717.1"
                     /translation="MSDHRNADQPGHGGQPSPSRDASTPADPIELIDECLAAFPDGDP
                     RQKLLYKLRHVVTAQSVAQDRRDTELKKLNEVVAKLTAPANRVGLLVEVPAEGVARIV
                     VGGAEYYANIDPRLAAEELKIGAQILVNEAYTVIKALEYDRNGPVLKVAEILPDGRIR
                     FEQDMGRQALILQRSSDLLGVDLKAGDEVRIEPTHRIAIEKFENRQARTHLLDEVPNV
                     TWEQIGGQHQAIDAIRKAIEYPLLHADTFTKYQFTQPKGFLLYGPPGCGKTMIGQAAA
                     ASLSQLVRESHGQAASDGHTKNPPVTSGAFLHIKGPEILNMWLGESERIVRDLFAKAR
                     ARRKEGALPFIFIDEAESVLGTRRSMRSFNINNTLVPMFCAEMDGIESLQDVVIILAS
                     NRPDLIDPAVLRPGRIDRKIKVARPTREAAVEILSVYLTPALPLEPALLEQHDHSHDA
                     ARRAVIEQVVDSLFSRTDQNRVLSIRLRNGQNKVLYRGDLVSGAILSAIVQRAKEKAI
                     ERAVAQTGAAAGDGIRAQDLLDAVQEEYREGEMLPPDDAAEEWLKLLDHHPEQVVGVS
                     SFRRGRPTEERLVNQII"
     gene            complement(16596..17696)
                     /locus_tag="PLS35_03340"
     CDS             complement(16596..17696)
                     /locus_tag="PLS35_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNI17718.1"
                     /translation="MSVAWACGVGLLSSVLSGLFSPAGGIAAEATSPSLTAQVAAPTD
                     LSPEEQATIAVFDRATRSVVFIANTAMQRDPWSFNLFEVPQGSGTGFVWSRQGHIVTN
                     YHVIYGADSITVTLADRTEYKAKVIGADPDHDVAVLQIQASEATLQPVTIGNSQSLRV
                     GQKVLAIGNPFGLDHTLTTGVVSALGRTIKSMSNRTIEGVIQTDAAINPGNSGGPLLD
                     SGGRLIGVNTQIVSPSGAFAGIGFAVPVDTINRIVPELIKHGKLIRPGLGISLVPDAM
                     ARRWGVKGVIIGKVGRGSAAERIGLHGARETAGGRIELGDIIVAVDGKPVESIDDLMD
                     SMEQHKVGDQVTIEFARGNRRMQATATLQPVN"
     gene            complement(17724..18146)
                     /locus_tag="PLS35_03345"
     CDS             complement(17724..18146)
                     /locus_tag="PLS35_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNI17719.1"
                     /translation="MAVKTVTIRVPMQGDSRMENITAQVQAALEQTPLRAGIVTVFIK
                     HTTASVLIVEDEPGIRADTQVLWERLIPADPRWQHNERNAGEDNGHSHLRGQLQGQSL
                     TIPFTDGAMMLGMWQQIVVLDFDTRARTRELVLQIIGE"
     gene            complement(18150..19022)
                     /locus_tag="PLS35_03350"
     CDS             complement(18150..19022)
                     /locus_tag="PLS35_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17720.1"
                     /translation="MARTRTTSLPLLVPRAFASTLLRFYDYPDPRRPGRMIKGYDCPH
                     AVRTARMCAAVAQRLGHAPARVKQYQIACLLHDLGRAGLDRTLFGRIWSWARAHGIPT
                     RPREWRAAHPDTRYGKETEAFVARYRTAMETAGITLDPWACEQVEMRLGYARRLAGRL
                     RIVKPELRDLGVAWAPWMGRIMLYYYYPEKLAGAQPWVRQLAEILVACEQFEAFSNQR
                     RGRDYYVRRREDLAEAFAYLNGLQKEGIISRPVVQALRELSAEGVFDAVLEQARGRAL
                     SKRERAFLRQASKE"
     gene            19183..19803
                     /locus_tag="PLS35_03355"
     CDS             19183..19803
                     /locus_tag="PLS35_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17721.1"
                     /translation="MRIPRSMQILAAGACLLLADTAAPVGASDQLVDVTGKVTPFVTL
                     RSRDNFSSEYRYDVTVRNSSPDLFVADSLILVLDHITNTAGQDRENLTGESLLTRMEI
                     IGQDGTTEDGKPYFRIPQGSGPDLTPNSESLPVTVRIRNRDYVAVFTPSFRVMGQRRE
                     PPKQKSQAAAAAAAAAPHKATTDKLIQLLLKKGLITAEEARTLLQP"
     gene            19800..20249
                     /locus_tag="PLS35_03360"
     CDS             19800..20249
                     /locus_tag="PLS35_03360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNI17722.1"
                     /translation="MSDHACKACVGTWPSQNHFIADCGLTKAYLHDDQFFPGWTVLVF
                     KRHATELFHLSREERSSLIEEVAQVSALLAEEWRAVKINYELLGNQLPHIHWHLIPRL
                     SQDPAPLEPVWRIAHEPVRPEPDALKSLVARLRAIWPAHRHQPPSSA"
     gene            20263..20925
                     /locus_tag="PLS35_03365"
     CDS             20263..20925
                     /locus_tag="PLS35_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17723.1"
                     /translation="MMQIPPGPNGSSPRTFFQTLIRTLPLHVLVTTSIVGGAALFYSL
                     SVSNRGTWTMLGSWSLTALYILIGLVGGTVAGVLDASRQMVERLEQVLRDWLHTLPAL
                     NRSADTTGRDVSTVRQEYETLVDHSVAQAGRRLRLPQWLERLIRAGVRGLVVDRFIAS
                     CTARGIHVVAPQEFRNWMLAEGVSLGFMPVLDQLAWWRYLLLGVLTLLAAIALTLAFL
                     TT"
     gene            21085..22428
                     /locus_tag="PLS35_03370"
     CDS             21085..22428
                     /locus_tag="PLS35_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17724.1"
                     /translation="MFLQRVSVGRWFLIATVALPLPSLPAGAQEEPSPAAATGTVQAE
                     TPVTETPAAPQADQAPQEAIRVEPLPSEPPAPPPPPPVAPPVQPPLTPLEILARARQA
                     LHIEPDAQEPRLTLGRTLFQLGDTDGAIDEYRTALRFHPTVAQAHLDLGTALMAKQDW
                     RTAMTELQEAVRLDPTLVQAHYSMGTIQYTRGNIQSAIKAYQEALRLKPDFAEAHYRL
                     GLVLKVAGKDKDAAQELEAAALAGLSKAQYFLGNAYRSGQGVEKSQIMAITWWAQAFE
                     QGLPEAAQALTQLRRLAAVKGNLQTKQSKAAAEAFKNYCDQIWLDFPDLDRDQPTETV
                     GTTLLKQGRTAEALPVLLREAYALNEMAHATLVQLYEQGLDGQLPPHGQWILSYLEST
                     AADGAVPSRLALARIYGKGLGLAPDQAKAKSYLKGLPKDDVKRILDDVAAEPPKP"
     gene            22469..25171
                     /locus_tag="PLS35_03375"
     CDS             22469..25171
                     /locus_tag="PLS35_03375"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI17725.1"
                     /translation="MRIATRLFVRNIWLQAAVMTLAAIVLSDILWTLAPATRQALEWA
                     PYDTWMRLRVQAAPDPNLLLIVRDRASDQQFGTGSWDRALPARLITALHDAGAASIGL
                     DIPLELPSPPNLGGAVSDALLMEAVKSAGTVSYPAIGVAVSDQEAAALSSHFTPGRSA
                     LHPTFDPDRVVRRLPLYHGDGPGELPALGFSLATTFWQVQSNQLERRSGRIHLHNARV
                     AGGSTGDIDIPVDRYGRLLINVAGHPLPTVFPTLSVLDLTRMIDRKENEGLSALVNGK
                     AVLFLTQPTPQPERMMPTGEEAPDMLLQAHLLHTLLTQDWIRDLSPLQRAIVTFALGL
                     STAWGVLRWTDWKGPVLGASMLVLYFFVSWAMLATAHWVLPFIIPVTTSVMILVVGSL
                     LGQMMATRRLVLLEQDMLLVQQNLVAVREALIYRETAVESLEEDLESARASMSHSVSK
                     EAESSRLAAELQLKIAEAQAQEDATRQRMAALEQQLQGMRAATITAEPLGNVEQDALR
                     RECEQAGMITRNPAILTMFRDLKKGARTTVTVLITGEAGTGKELCARAIHRLSPRAAK
                     PFIAVNMAAISPELFESELFGHVRGSFTGAHADRKGYFELAHLGTIFLDEIGDLRLDH
                     QSKLLRVLQDRSFYRVGATTPTTVDVRIVAATNKDLQRGVSEGWFREDLYFRLKGLVL
                     DLPPLRERRDDIPLIAEAYLHEAANHNHQSSMRLSEEAVSTLMQQSWPGNVRELRQCL
                     EQAMALSDSPILSAADLRLSGPASPKTGTSGAAPLLPDPAGDLAVLQQLRQQRFDMQA
                     TAKALGWDRSTVTQRLKGLCFQALVDASGDQAKAAASLAGDPSLLRTVELKLMDYYGH
                     LMETIAPFTNVDDALLDCKRRFKNLPERHFKSVEVLVRRHFSAA"
     gene            25274..26395
                     /locus_tag="PLS35_03380"
     CDS             25274..26395
                     /locus_tag="PLS35_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17726.1"
                     /translation="MTEPAQVTWTEEGQSYAAHWRSEWGAPPPKRVVIADDRMTADAA
                     HRLACEGTALLWRGDFQNARQLLLAMARRADRHPPKPGASPTESFHFHRQTQARRARI
                     LGMLLITLEDDYAIRLRRAPDLREACTEAYGPATARALVSLRELLGVIGAHEWRKNGI
                     DIPALGDRIHPHYGVFSPIRSEYVALVADAPLPACTLAFDIGTGTGVLAAILARRGVP
                     RIIATDQDPRALACARENLQRLELSDQVEVRQANLFPEGKAQLVVCNPPWVPARPTSP
                     LEQAIYDPENRMLYGLLNGLAAHLEPGGEGWLLLSDLAEHLALRSRKELLATFEQAGL
                     VVAGKTDIRPTHPRASEQADPLRAARAAELTSLWRLKSR"
     gene            complement(26431..27237)
                     /locus_tag="PLS35_03385"
     CDS             complement(26431..27237)
                     /locus_tag="PLS35_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17727.1"
                     /translation="MKRLSSWLLMSWLLMGSLVQAADYQLTLGQGIDVCEACKQNLER
                     MTVHPACEREYSAQFDLESPAWKPFDVAGHLEAMEQVLYFLTAGSESGRNIPDESRFE
                     DTLWRISTKDEPWAYWAQVDINNDGKAEPVLKLHSSLCHARNDGSIGRAYSAPIVVLT
                     ENLSGIDHELTDLVLQNPRQDGRAAGETNYQLYGVFRYKGTTYFDRWDDAGSGDQADQ
                     FTLSIYASTGDTTRKLCRLKELQAKRWFPGLPCTSRQCQTARPLPAHYIP"
     gene            27891..28709
                     /locus_tag="PLS35_03390"
     CDS             27891..28709
                     /locus_tag="PLS35_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI17728.1"
                     /translation="MQKSPIATVSAGPVAPTLVGPDRDMKDVYILSGLVGFLAIVVAA
                     FWYYSQADEAAHSNRSSEALDVAQVSQVLKESTEKVTGGAAMPVTHVETIPLASRSTE
                     ILHDDIQFEVGRKGLTDDGKASLQRHAEFLKNEPDWGVLLQGYTDQQGSMSFNKILGM
                     KRAETVKQQLISLGVPESAIRTVSLGEEGALCIDKSDACRQMNRRVHLEMRKIGQEHM
                     VIPAVITEPVTDSFSLTTDLSMESASAESDSENPTGVGASSNGADSPVESATGN"
     gene            28868..30925
                     /locus_tag="PLS35_03395"
     CDS             28868..30925
                     /locus_tag="PLS35_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HNI17729.1"
                     /translation="MALATSGVGTPPTDAGSAESPVTLSTGVVPTMGINEVTEGTLLF
                     RTDQAGRFTPAPVLKTDVHIAVTGTIARATVRQEFTNPSTRKGDWLEGIYVFPLPETA
                     AVDHLRMHVGERIIEGQIKERAVAKKVYEQAKQQGQRTSLLDQERPNIFTTSVANIGP
                     GEHITIEIEYQETVRYDDEQFHLRFPMAVGQRYIPGTPVIIEGQAATGSGTMLDTDRV
                     PDASRITPPMQSPRYGAINPLSLSLTLHPGFPIDTMESSFHPIISIPDAEGGYHVTLR
                     ADAVPADRDFQLSWHPVARTEPIATVFTEQKNGQTYAMLMLAPPTQHDEKAPRVPRDL
                     TFIIDTSGSMAGPSIDQAKRALAAALPRLSMQDRFNIIQFNHTVRSLFAVPQPVTTTT
                     IKQAIRYTEHLSADGGTEVLPALRQALKSPQESARLQQIILLTDGQVGNEEELFELLH
                     QRVGTRRLFTIGIGSTPNSHLMRKAAEFGRGTFTYIGKVEEVEQKLERLFRKLERPVL
                     TDVSVDQAGWSGLEQYPATVADLYEGEPIVLAMKADAVPSHATLHGQVGSRPWSLPVS
                     LNQTATQGGLSVYWARQKISSLMDEATKEGRAEVTKTSVIEVALAHHLVSQYTSLVAV
                     DVTPARPTDVPGAEPGQTTNLANMQLSALPKTATGWQLQLLLGLSALVLAALVRRLRN
                     EVA"
     gene            30922..31524
                     /locus_tag="PLS35_03400"
     CDS             30922..31524
                     /locus_tag="PLS35_03400"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HNI17730.1"
                     /translation="MTGTWHRSRPVVWLMASLLAIGLWQVWEGSWMYAKAGLAQLLLQ
                     RAWTRSVEGEAMPKPWPWADTWPVARLRIPRLSVDLIVLAGAHGRTLAFGPGHVGSSA
                     LPGQTGTMMLAGHRDTHFQFLKELQPDDRLEIVGTDGTTRRYRVTERQVMDSQRDSIA
                     TSDERAELILVTCFPFDAITTGGPLRFVVRADAIPQADRS"
     gene            complement(31577..>34164)
                     /locus_tag="PLS35_03405"
     CDS             complement(31577..>34164)
                     /locus_tag="PLS35_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNI17731.1"
                     /translation="EYHVELDPGQLMSYNVTLDQVMDGLAKSNTDVGGNYLSMGEQSF
                     NIRGMGLIKKLEDISNTVVTQKEGTPVFVRNLGKTSIGSRVRLGKVGIDDRDDVVEGV
                     VLLQRGAKALPVLDKVHEKVQELNARKLPQGVAIKTFYDRTVLIHTTIETVIDILIAG
                     VVLVSIILYVFLGHFRTAMIVALTVPVALLFTFGMMVLSGQSANLISLGAVDFGIIVD
                     STLIMVESIFYHLAHKSSAGVTVPMHVMRAAREVGRPIFFATTIIVIAFLPLFTMTGV
                     PGKVFAPMSLTYGFALTGALLMAFTLAPALCSLLLTGPIKERDTAVVEFLSRRYMALL
                     NWGLQREWLVIGIAIVTLVLAMAAVPFIGGEFMPALEEGNIWMRTTFPVDISFEQAAR
                     LVTDIRGIFRQFPEVISAASQLGRPDDGTDPTSFFNAEFLVTLKPFKEWRADVPTKKA
                     LVDQIEQRLAVIPGVTFNFSQAIQDNVQEAMSGVKGENAVKLYGSDLQTLEQLAAQIE
                     LVMKDVHGVKDLGVLHLLGQPNLVIEVNREECARYGLKVGDVNDVVQAAIGGQAVTQI
                     YEGERWFDLVVRFLPEFRRDIESIGNIVVSTPDGARIPLKQLASITEQTGAFIVYREN
                     NERYIPIKFSVRGRDLEGTVREAQERIEQQVPLPAGYRIEWHGEFDQLQEEKARLAKI
                     VPITLLLILFLVYLVLNNVRDAFLVLAAVPFSLVGGVLALLVTGTHFSISAAVGFISL
                     FGVAVQGALILISRMQELLREGYEIRDAIMKSAEVRMRPVLMTSLAAAIGLLPAAIAT
                     GIGAQSQQPLARVVVGGMLTSAALILVVLPVLYQLLHRYRKPSRSTGATASAGTANQG
                     HGSHE"
BASE COUNT         7259 a        10806 c         9447 g         6652 t
ORIGIN      
        1 ggagccaatc tagacacata gaaaggggct gtcggtatgc tgccgacagc cccttattct
       61 atctgaagcg tgagcgttag actttctacc gtgtcatagc tttccagatg accgtgcagc
      121 aactccgctc ttcagcacga tttcacaaat gtgatcgagc cgctcaatgt gctcataggc
      181 cgcccacgga tctgtggcga cggcgcacac gccgtgattc ttttgcccta cgatgtcaaa
      241 ctccagggtc ccgtcttttc tcagccctaa acattccgcc gtcacatcgg ccaggtcacg
      301 agataacgca ggcaatgcgg gaactgtacg gccaacacgg gtatagcggg aaatctcagg
      361 gaattcagcg ctcatcgcct gtaaatcaat tcccgcatac atggcggcaa cgatatgggt
      421 cgcgtgcaca tgaacaaccg tgcgcgtttt tttggtgtcc cgctgcaaat tccaatgcat
      481 ccaaagttcg cccgaaggct gctgcccctc tcgtacagat ggcaccaaat gaccgctggt
      541 tggatctgtc acaacttcca gcctgaccac atgttcagga tgcacaatgg tcttccgcca
      601 tccagacggt gtgatataca ggtacttccc atcccgcttc cgcatgctga tgttgccatc
      661 tcgggtggtg atccagccgc gctcgtacac ccgcctcatc acatcgccga ttgccgtcag
      721 catagctgcc tcctgtaaga aacaaggatc cagcatctgt tatcggcatt ttgctttcag
      781 gccttaagca ggacgcagca ccttcagctg aaggaaaatt tgatttgggc aaccagatcg
      841 acttttggct atgatgacta acgtatgacc atcctcaacg gatttacccc tgcattgctg
      901 ctccgaaacc cgcatttgat gacgctgatt ccgcgatatt ggcctcgcct gagatggaaa
      961 gagaaccttc cttgctgtga acgtctcttc acgactcaac caggcaccca gcttctcggc
     1021 ttttgtcatt ggcaggatga ccccacgacc gcaccaaccg tcctcctggt tcatggcctt
     1081 gaaggatcgg ccgattctca ctatatgcga gggatggcgg ccaaagcctt ctgcgcagga
     1141 ttcaacatca tccggatgaa ccaacgcact tgtgggggca cggatcacct gactccaact
     1201 ctctataaca gcggactcag caatgattac cttgccatca tacgggaatt gcgggagcgc
     1261 gatggcctga gccatatctg gctcgtgggg tactcgatgg ggggaaatct ggtgctaaaa
     1321 gcagcgggag aactgcaacg atcggaagcg gccctggcag gcgtggtcgc ggtcagtccc
     1381 aatattgatc ccacgcaatg tgtggctgct ctggaagagc cgagaaactg gctgtatcat
     1441 gcgcactttc tttcgagtct caaggcccga atgcaacgga aagccgctct gtttccaggc
     1501 caatggaatc tctcgcctct acgcctcatg aagacgatta ctcaattcga tgacacgtac
     1561 accgcacgcg acggaggata tcgagacgct acggattatt ataatcgggc cggcgctcgc
     1621 cacgtgctat ccgaaattgc ggtgccgacg ctgattatca ccgcccagga cgatcccttt
     1681 attccggcat caatgttcga cagcccgggg atcaaaaaca atcgctacat tacgctcgcg
     1741 atgctacgga acggtggaca ctgtgggttt tttcagaagc gccggtatgg agaagatcgg
     1801 ttttgggcgg aaaacagaat tatcgagtgg ctttcttctc gggcataatt gagacggaga
     1861 atcgtcttag cggccatccg caaaggcctc caatgatttg tgcggaacct gagacagcac
     1921 gctcagctca ggcaggtgca cttcctgtct cacgtccggg ttttgagggg tgagtccggc
     1981 ttgctgaact gttgctccaa accgagatga ggcctggcca aaaatgatcc ggcaagtttc
     2041 cggccatcat cataaaccgg ttgtgccgcc tgccactccg ccatgggcgg gtttaccgga
     2101 aggcttacca taaacttggt gccgtgcccc acctcactga acacttccac atagccgcca
     2161 tgctcctgga caatttggtg agcaatggtc aaccccaggc ccgtcccctc acgctcgccg
     2221 ctttcatgct tggtggtata aaacggatcg aaaatatgat ccagatcgtg cggctcaatg
     2281 cccgggccgc tatcctccac ctcgatctgc acccacggct cattcacaga tttgacgagc
     2341 tttcgtgtac ggacagacag gcgtccgccc tgttcgcccc cgatggcttc catcgcattg
     2401 ataaataaat tgagcagcac ctgtttaatc tgctgacgat ccagcttgac gtagggcaag
     2461 tccggcgcga gttctttttg aatagtgatg gctttgctgc tcgccttcac ctcgataaaa
     2521 tacaagcagg aggacacgac gtcgttcaag ctctcctgcg tcaatttcgg cgtcatatac
     2581 cgtgcatagt ccagaatctc gtgaaccagg cgttcaatac gctcgacatc ctcacagacc
     2641 acctgactaa actgctccat aaattccaca tcatcccgcc ggtctggagc cagttgcacg
     2701 aacgtcttga tggaggtcag cggattccga atttcatgag caaatccgcc cgccatggtc
     2761 tccagtgacc ggagtcggtc ggtacggcgc atcaacaact gcgaccgtcg caggtcttca
     2821 tacaacaggg cgttatcgat cgcgatggct gcatgttgag ccagcgtttt cagtagcgcc
     2881 aactcttcag tcgaatacat gccatgattg gcacggcgcc ctaaattaca gaagccgaca
     2941 agccgctcct tgctgaccaa cgggatacac acttccgact ccaacccctt caatgtatcc
     3001 agcaatcgca ggtagtcgac atccgctttg tcgtattcaa tctcttctcg gaccagagcg
     3061 gtttcggtcc gcaacagggc ccgcgggaat tcgctctcca tcttgatggg agggacgatc
     3121 acatctcgcg tcacaaaccc ataggaggct gtcaaactgt acaccccctg ttctttgtcc
     3181 aggacataca ctgacgcagt cttaatgttc atcacgcggg ccagcgtctg tacgatttcc
     3241 tttgtgagcg atttcagctc aaggatcgcc accagggact tggaaaactg cacgagtgtg
     3301 tcgtaggcat catagcgctc ccgaaagagg gtctgcccga tcgcacgctc cacgcctttt
     3361 tgcagattgg ccaaaccact cgacacaatg acaaagagcc cgaagagcac tccggcaagc
     3421 agatagtgga acgtgccggt gaaaattcga atcaccagca atatcatcat gtaagccgga
     3481 atcagacccg ccagcttgat cgataacatg accttcggcc tgctcggcac gaacgagata
     3541 tccatcaatt cgtatcgcgc gatggcgtag gcgacgaccg tgacggacag tcccgccagc
     3601 aaatagccaa ccggatagaa cccgatgcca tagttctgca agaagtcgag ggaggccgag
     3661 aagccgagta acagcgccac gaaggtatat tttaattggg cgcctaaaag aggagcggat
     3721 gtttgatgga accgatagcc ctggtagagc cgatacaccg tcagcccacc gcctcccacc
     3781 acataggcga ccaaccaggg gtggagcacg ccggccttcg gataccggcc ccaataatag
     3841 acatactgtc catccaccac atacgtcgtg aacaagagac tccagaacag cccgccgagg
     3901 gcgtagttcc acccaatcgc ccgatccggc accgcccctc ccgcaagccg ttgcgcaaag
     3961 tgataggtca gggcgggcaa aaatacgact cccgtataaa tgactcgcac ccagaacagg
     4021 gcctcctctt cgtgggtggc agagaacaac atgaagcagc cgaatagcca caggcacgca
     4081 gcaccgcata cggcgctaaa caccgtattc gaagatgagc ggggattatt gctgagcacc
     4141 cataaggcac agatcaaaaa cagagtaccg gctaataaag gaggagtgga gtgtccggtc
     4201 gcccgattgc tgaggacggc gccgatagag gtcgccacca caataatcgc cagtacgatg
     4261 gcatatccca gccctttgtt caggacgacg ctgatattca tcaggcgata ccgaacgatc
     4321 gtatacgcca tcaacgccgt atacatcgtg atcagcaccg tcccataggg caacagcggc
     4381 accccgtacc agagcgggaa attcgtcgcg ccgccggcat agccaatgac actggctgcc
     4441 agcatgtagg catattgatg gcggcgaatc ccgttggcat cgcgaagcgc gacaccgaca
     4501 agtgaggtgg catagaccac gtaccagaga aaatacgcga gaaatggatg gaagaacggg
     4561 cccggtaccg gccagaagga aaaagacatg gccggatgca cgccggccac caaccaggga
     4621 gtcgtatcga ccagcaggaa cagtccggct agtccatacc cgattttcag cgccttggca
     4681 tggcgaaccg caatgtcgag aaacgtgact gtgtggtgaa aatacaggat ggggatgaga
     4741 gacgcgccgg ccatgaccag gcgcaacagg ctcagcgcga gatctttcga ctccgccagc
     4801 tgccacccgc agtaaaacac gctccagaac gcgatgctca gacagtacag cccgtagatc
     4861 cggttgcgcg cagcggcagg atcccggaaa tagacgaaca ttccgagccc tgtcgcagcc
     4921 aacgcattga ccagcgcgct gattgcgagc actttcatat caagtcctcc gggaggaccg
     4981 gagccgtgtc atcatgcagc gaccgtgacg tgcgccatgc ccttttcggc cacggcaatg
     5041 agggcttgat tgccgaacgt ggaatacact cgcgggcgtg agactcccgc ggctcgcagt
     5101 agactttcca gttcagtctg gtagtggaaa tgaaacgtgc cttcgccttc gcgttccttg
     5161 attctaccag aattgtccag caggcgacga ccttcctcga cctgatcctt cgtctgagcc
     5221 gtatccacaa aattgcgata aatggccgat agatcggcat acggtttcaa attggaaatg
     5281 accaaccgcc cctgtggagt gagcacgcgc aggtattcac gtaaagtgaa cagcgggtca
     5341 cgtacgtagc ccagcaccag gttgcagacg acgcggtcga aggagtgatc gggaaacggc
     5401 agcgcgcttt ccaggtcagc ctggcagaca cgcatcgaca tgggctcata ggcccgcagc
     5461 ccttcataaa actgcccctg cagcgtcgca ccgacctgct cgaaattgct catggcttga
     5521 gccaatgcag ccggcacgaa gtcgagaccg acgtaatcgg gagagcgaaa atcgccgcgc
     5581 ttgccatacc gctgccgaaa cgcctgattc aattgcagga agaccccgaa attgccgttc
     5641 ccgcaaccgg catccaaaat gcgctcgccc ttgtggcaat cgcccatcaa tcgatagaga
     5701 tgatccatga gccgccagaa atcggccaca ttgggaatcg tctgaaagtt ctgtaaatac
     5761 tcctgccaaa acgccacgtg atcagacttt cccatcggct tccgcttctt gctccgttcg
     5821 cgctcaagcc ggttctgcgc accgatttca cggtgggacg gttcgaccat tcgctccggc
     5881 cgccgcgccg gccagagttc ctgttggcaa cagacggcga tttgccggta cacggtacgg
     5941 gctttccgtg gactttcctg caggcgatgt aatgcgcccg gcacaacgaa ggagtggcgc
     6001 aaatacggac cgattgcctc agtcacccgc tcaatagaag cgggatcgac ccaggcgtcc
     6061 tgctcggcat gaaacaggac gaccggagtt tggagacgcg aggcatcccg ctgcgtcgtc
     6121 aggagatcgg catactcgcc ctccaccgca tgcgccaacc atcgatccgc atcgatggtg
     6181 agccccatga tgttcaccac gcctttgtgt acacctgaca gatgctctcc aatcaaatcc
     6241 tcttgatgga cggcctgcag cgtatgccgg acatccataa ttccgttgat gagggtcaag
     6301 agccgcacat gcgacacctt gccggccacc ttcagcgcga cgcgtcccgc cagactggtc
     6361 gccacgaggc cgatcggtcg tcccggccat tgattggcga catggtccaa cacggtttcg
     6421 agatccgcct ccatgtcctc cagccggaat tgcgtcacca gtccgtcact ttccccgaca
     6481 tgattcacat tgtcataacg gacaacgtgg aacccattgc ccgccaggta atacgccaag
     6541 ggcacataat cgcgcttgct ctccccatat cccggcgcca atacgataat cggggcgtca
     6601 gctgccgggg caacttgtgg ccggtcatgg cagatgacga tccggaggcc ggatggtcgg
     6661 aaacattctg agaactcact gagcacggtg ggactgccca cttttgtttc agggcttggc
     6721 atcgacacct gtcccaggaa atgttcgatg atatcttccg cttccttcgc aaccgggaga
     6781 aagcgcaccc ctgccagcac ggtacgccct gccacaggga cgcacccggg gcgagcctcg
     6841 ggtgcaactg gtctggtcca caccacttcc cccaccaccg agcacagggt tgccgacaaa
     6901 gaatcggtcg aagatccctg gaggggagcg gatggcaacc atcgtaattc gagctccgat
     6961 cccaagagat cttcttcagc cgataggtgg acgcacgcgc cattgggcga caagtccgtc
     7021 gtcaagccag cggacaacgg ccgatccccg ccacgagttc gaagactcac ttccgtgctc
     7081 aatccaacaa ccacccgctg ctgtcgccgt cgctcttccc tcgggcgttc tggccctcgt
     7141 accagttgca tggaggccgg aggcgtagcg gcggtctgta atgaacccgt atcaatgagg
     7201 gactcctctc cttcctgcac gaccaaggcg gccgccagac gcatgtgtct tggcgcagta
     7261 gcgccatgac tgaggagcga tgccacaacc cgttcatctg cgtcactcag gtgtatgaac
     7321 tggatggcca gtgtcaccgc cgcttgagtg cgaccaggta cgtgcacatc ctcggacgag
     7381 cgaataccgc acacgatacc aacgctgtcc aacccgcgct catccgggct aaagctcaac
     7441 atcatccgct gattcagcat ggcaggaatg tcggagacaa agtccatcga gaggccgcca
     7501 agactgaggc tcctggtcac ccccttccag gtgtgtccct gaaaaaacaa ctgagtggag
     7561 aggcgaagcg ccagacgacg agacgctcgc cgttcggcga gagaaagcga ccaggcgtcc
     7621 tgcgttttct ttctctcgac ggccggaacg ttcaggctat cctcatgccc gcagagggca
     7681 gataacggct gagtcacatt acgcgcagtg ctatcagacg acatacattt cctcccgcta
     7741 gtttgagtcc tggtgtcggt cctgccacag catcgtggtg agtaacccgt ggccatctcg
     7801 ccccgtcatg catcgagcgt cgaggcgagg ctggactctt gcaagcgcac gaacgattcc
     7861 aaaacagcgg gaggatgtcg gatgatcaaa acggggaagg ataagcggca cgcacagcct
     7921 cagtgtaggc acacagcgac tagccaatct gtctgagatt cgaagcttat tctaggtgat
     7981 tagacataat tgtcaactca tcagtagaaa agatatcccg cggcctacaa gaaataacct
     8041 agttgttagc tgcagggctt cccatccccg gtcgcgccgc ctacggcccc caacccgctg
     8101 acaggaccag tcagagcact gacgaccgtc atgatactga cgcggatacc tggcatacgt
     8161 aattccgaga cctaccgtat ccactccctt gcactgacaa cgacaaggcc cgtttttcca
     8221 gggagctgat ccgcatcctc ggacatcagc cgcctacgct gctggcagtc tttccagcga
     8281 atgggattca acggggtcca cgtagtccag gccggctgtt cggttgcttt gaaacaaaga
     8341 gttatgttac ccacagggcc tggctctcta gccctgtcac tcatcatgca gactgcctac
     8401 gtccggctct acattggctt gatgaacatc ctcctgaccg gcttcgtgct tggttgtcaa
     8461 accagcccgc ccgtcacaga agggtaccct caccagcaag cacttcaggc aaaaaccctt
     8521 caagaagtac tggcctgcgc cgggaatccg ttacagaagc gtgaagacgg gccggtcacc
     8581 atgctccgct attaccggga ggcccccctc ctcgaggaat ccatggtgtc atcgaaggga
     8641 agccggccaa ccgtccatca tggctgctgg gcgaccgtga tcctcaaaga tcagcgggtc
     8701 gatcgggtcc gataccattt cgtgcccagt tctgttgatg cctccaatga ttgtgaagaa
     8761 atctttgcga attgccagga atgaagaccc ggcgcttcaa cggtcgcaag atcctgggcc
     8821 tgctgctgat ggcctggtgc ctgcaatctg tcgctgactg gccccctcct gcctacggtc
     8881 tcgaaaaata cggccggcca cttccctcca tggatgaacc ggaacacgaa gcacgggagg
     8941 aggaggaaac cctgttcggc ggatacttcc tgacgggggc gtttgtgaag aatccgacct
     9001 ttgccgcccg ccctgacaat accggtctcg tcggcatgcg gcacatgctg catctggaga
     9061 cagacctcta taagcaatac cttacctttt ataccgatca gaactttttc tcggatcgca
     9121 ccgacgggtg gatcaaactc tctgagtggg acggcacctt cgcattcacc ggtgtgctgg
     9181 accgattcaa ttggcggtta cagtatgaac gcgatgcgcc cctcgaccgc cgtggcttga
     9241 agcaagccta tgccgacgcg ctcgtgacgg cacgatttca ggccattcag gattccacct
     9301 ggtggcggcg cacctttcct catcagaatt tcaccgcgta tgccggagct gggtggctct
     9361 ttcataacag ccagtacttc gccagaccca ataataccgg ccgcgccttg ttccggtatg
     9421 tcgcccatgc cgacctggat gtctataaga acaaggtcgt gctgtatggc gacgtaaacc
     9481 tcttcaccga ccgcgaagcg agtcagaccc tgaacccctc cgagctcgac tggatcgtcg
     9541 gtattgccgt gcgctggcag gacatggaac tggccttcta tcgcgaggaa gatcggccgc
     9601 tggatcaatc agggctggtt caaaagtact ttgcggtgca gttgcgcttt gcgttcgatg
     9661 tgtccaaagg tgcgatggag cgagtgggat ggaagcggta ggccggaact agcgtgcact
     9721 gagatggctt cgcacgtcct gcacgtaggt tttgaacggg tcggccataa aaaacggctc
     9781 ggtatcacgc aacttaaaat tggaccagtt gataatgtat tcacaggagg catggtcacc
     9841 aaaactatgc gccgcatctg cccccgcccc actcgccgtg gacgccagca gatgccgcac
     9901 caattcacct cggccgaagg cccgcgtatt gcggggcggg tggtcctgcg ctaattgaac
     9961 gcccttgtct gtcgtgaggc gtggtccccg cccctcctct tcaaggccgt agcagagtcc
    10021 ccgctcggga tgcaggttgt gatattccag atcgaggctc ttcagcatcg ggtcgtccca
    10081 cgccaactgt tccgcctccc ggtaggtttc caatagccag agttttgagg cccagtcgat
    10141 ccggcccacg agtttttcat acccgtgccg caggtcggtc aacaccgact cccattgctc
    10201 cagcacccaa tccgtctctt cgtcctggcc tttgcaatgt cgctgcgcgg cggcaagaaa
    10261 ctgttcttga atatcgagcg cagagatcga ctgcccggat tccagccgga caatccactg
    10321 gcgatccggg tcccgtgaaa tgtcctgcaa cgcctcaacc ggctcgctga tactcaatcc
    10381 acggggagcc tggcctgcct cgatcagttg caggaccaac cctgtcgtgc ccatcttcag
    10441 tgcggtggca tattccgcca tattcgaatc gccgcagagc aagtggatgc ggcggtattg
    10501 attgggatcc gccagcggct catcgcgcgt attgacgatg gcccgattgt gctgcaccca
    10561 ttcaaagaaa tcgttcacga tataatcggc ccgctgggag atctgaaacg ggacaatcga
    10621 tcgatcctgc gcgtcccgca accccggacg atgcaagatg agcccgccga tttgcaccca
    10681 ttcattagga tcaccggcgc aaccgatccg ccccgcgccg gtaaacactt gacgggtgac
    10741 caagaaagtc accagcggac caagaccgcg ccgagaaaag gggaactggc gagtgactaa
    10801 atagttttca tgcgacccga acgtcgcatc ggtttcgtgg tcgatattgt tcttgatgag
    10861 cgagaccgtc tcgtccagtc ccaaagcagt cacggcctgt tgaatgatcc ggtctccggc
    10921 gcgatctgat gccacgagat ccgcaagggt cgtgcattca ggcgaggcat actccaaatg
    10981 gcccatgtcg atgtagatgc gcccggcgtt cgtcagaaac cccccgttgc ctggcggctc
    11041 atcatgccca cgatggtgca gatcgagcac gccgcgacgg gcgatgtgaa aaatgtggtc
    11101 ccggatgcgc tgcgccaccc aggaaggcga atggtcaggc cgatcctgat tgaccaggag
    11161 gccatattcc gtttcgatcc cgaaaatacg attcagcata ttcagccaac tatgggtacc
    11221 gggctccaga acgtgatcga tgccaagccc atttgttccg tcttacctcg ggaggagatc
    11281 actgtgaggc tcgaaccgcc gacggcagca cgcggcctaa ttcgtccggg cgaagggtcc
    11341 gatatttgga cgaaccaggg ccattgcgtt ccagcagcac gcattccaga gatttttcgg
    11401 ccacggtctg ccgcacatga gccagcaagg ccgttttgtc gggcaaggct tcagcgtccg
    11461 gtttttctgc cgcgtcgcga acagaggagt cgccatccga tgtaccggac gaggtttgcg
    11521 ccagagatcc caccgcccac gcgcagagac cggtttctac ggcctgcgcc aatgaagcct
    11581 gggcaaaccc cggctgtgcg tcgatatacc gacccatttg aacttgcacg gcggacgtcg
    11641 cacccaacgc agcccagccc cgccgttcct caaagttgcc atcgtagttg atggtcagaa
    11701 acagatcccg ttccggtttc acgcccaatt cggccaagag aattttggcg atgaagggcg
    11761 ccttgtaaat ctcttcaaac gcctgcttga tggccggcgc caggccgtac ttcatcagcc
    11821 tcgcacctgt cacatcggag ggcgatcgat tgaagccttc gacatgggcc atttccaaga
    11881 gcgagaaccg taatttctcc agatcggccg gatgccccat gccaccgagc gcaatgcgat
    11941 cataaatctc atagagcttc ggcgtgcctc ggctcacggt caacagcacg atgccgtcgg
    12001 cgtaggccaa cgccacgacc ggcgcaccca tcgcaaactg ttcatcgaga tactgccgcc
    12061 gattcccgac cgcctcgatc caccgatagg gttcttcata cataacacgt cacctttatc
    12121 ctgatcatct tagacccgcg aggtctccag ccttcaagtg aacatcgata ggctagagcg
    12181 cgcggacgac gtcacgctcg tacaagtgtt tcaaatgagt gtccggaatg gtctgcaccc
    12241 ctgccgcggt aataagcttc accaccggat acaggttcaa ctcacgattc acgccaccgg
    12301 tcgccgaatc gaactccgcc gcgctggaga gcaatcgcag ggcttgcacc gtcgcctgct
    12361 cttcaggcat ggctgcgagc ggttgagggc cccaggtgtt cacataatga aggatgcccc
    12421 ggatcgtcgg cgagccggag ccggacacgg cgtactccac cgcttcgaac tcagccccga
    12481 gaatgtcgta gaagtagatc ttggccgctt cctgctcatg atcatacccg gcaaaaaccg
    12541 gcaccaccgc tccggttcca gccaacgccg ccggcacgtt ttctttcagt aacttggaca
    12601 cggcgcgaag tttgccctca aaactgagct cctgcagctg tgtgcgacgg taatacttga
    12661 atgaatgctc cagcacacga accatctcgt aggcagtggc cggcacaccg gcaatcgcca
    12721 ggacactatg gcgatcgatc tccaatacct tgtcggtccg gtcatacatc accatgttgc
    12781 cggcggtggc ccggcggtca cccgccacca gcactccgtc acgatacttg agcgcaagaa
    12841 tcgtggtggc gttcggaata tccattcccg ccgcagccgc ggacggattc ccgaactgat
    12901 aaccctgctc tttcaagagc tgaaaaaagt ccccttgcat ccccattgcc tacctcgtta
    12961 gacacacccg caaccgttac gtttcacgat gactatcacg tttattgtcc cgtgcgctgc
    13021 cgatagcgct ctgcctgctt ggggtcgact tttcgcatcc gcttcagcag attatccttg
    13081 tccggcgaac cggtatccgg ccgccgagga ccgccgcctt cttccgaagg gcctgttggt
    13141 ttcggcatcg gatcgccggg ggcttcacgg cgctccggca tcattgtata tcgcatcatt
    13201 gtgcatcctt tcgttttgcc cacgcagcca aggcatcagc aggagacgcc gcggtttcga
    13261 ataacccggc acaagcctgc accgcctccg gatcgaacag atcgcccatc tccagcgtac
    13321 gaccgcgcaa cccgcccgcg aactgcaccc gctcccactg catccccgtg atttgttcgg
    13381 gaaaccgtct gacacacagg ccgcgcaatc ctccacgggt atctgaggga ccagccacga
    13441 gcgcctgggc aatgtcgcgc tcggtcgtca tccgccaggt cttcccttcg gcttcgaggc
    13501 cgagaaacaa gccacggtca ggattgagat tatggtattc caaatccagg ctggcgagcc
    13561 agggatcctc ccacgcgagc tgctcctcgc gcatgaaggt ttccagcaac cactgtttcg
    13621 tgacccaatc cagcttgccg acaagtggtg tgcgatcccc cccgaggcac tgcagagtgt
    13681 catgccattc acggagaacc caatcggcat cgggatcgct ccctccgcaa acccggcttg
    13741 ctgcgttcca gtactcttgc tgaatagcca ggccggagat cgtccgtcca tccttcaacc
    13801 gcacggtcgt tttgagatcg ggatcgcgag acaattgctt gatggcggtc accggctgct
    13861 ccaactctac cagcggagct gcgccccgct ggagcagatc gagcaccagc ctggtggtcc
    13921 cgactttgag cgccgtggcg tactcgcaga agttggcgtc accgacaatc aaatgcagcc
    13981 gccgatactt tgcccggtcc gcatggggct cgtcgcgcgt attgagaatg ggccggttgt
    14041 gcatcgtgtc cacacccagc tccgcttcca taaaatccgc ccgttgcgag agttgaaagg
    14101 ggcccggcac aaagccggac tcctgggctt ccatgcccac cttccctgca ccggcgatca
    14161 cctggcggct gaccagaaac ggcaagagcc cctgcaccaa ttgtgggaaa ggcagagccc
    14221 gtggaacgag gtagttatcg tggcagccat aactgtgacc gtgaaagtcg gtattatttt
    14281 tgtagagctg aacatgcgcc ccgcccaacc gctgattgcg acgatcggcc gcgcgttgca
    14341 caatgcgctc ccccgcccgg tcatgcgcca ggagatcttt gagcgtccgg cattcagggg
    14401 tcgaatactc cgggtgcgtg tggtcgttgt aaaaccgcgc gccgttcggc aagaccaaat
    14461 cgctcttcat ctcatggaac gaatacggac gatgggcatc gaccttggcg aagtcgtctt
    14521 cttccttgtc ctgctgcaga cccgagacac ggaagccgcg cgcatcctca tgcggatctt
    14581 cgccatggta atcccaccgc cgttcgaact cgccggtcag atgggcccgc accagctcca
    14641 tggattcctc gaccggatcg accgcgtcga gatcctccct ggtgatgccg tactctgttt
    14701 caatgccgaa gagatgcata gtccgttacg cgtgagaaga accgcgttcc cgccgatcaa
    14761 ataatctgat tgaccaaacg ttcttccgtc ggcctgcccc ggcggaacga agacacgccc
    14821 accacctgct ctggatgatg gtccaggagc ttcaaccatt cttccgccgc atcgtcgggg
    14881 ggcagcattt ccccttctcg atattcctcc tgcacggcat caaggagatc ctgcgcccga
    14941 atgccgtcgc ccgctgccgc gccggtctgc gccactgccc gctcgatcgc cttttccttc
    15001 gcccgctgga cgattgcaga cagaatggcc ccgctgacaa gatccccgcg atacagcacc
    15061 ttgttctgcc cgttgcgcag acgtatggac aggacacggt tctgatcggt tcgcgagaag
    15121 agactgtcga ccacttgctc gatcaccgcg cggcgcgccg cgtcgtgaga gtgatcgtgt
    15181 tgctccagca acgcgggctc gagcggaagc gcgggtgtga gatacacgga caaaatctcc
    15241 acagcggcct ctcgagtcgg acgcgccact ttgatcttgc gatcgatccg gcccggccgc
    15301 agcaccgcgg gatcgatcaa atccggccgg ttggaggcca gaatgatcac cacatcctgc
    15361 aacgactcga tcccgtccat ctccgcgcag aacatcggca ccagcgtatt gttgatgttg
    15421 aatgaccgca tcgaccgtct ggtccccaac accgactccg cttcgtcgat aaaaataaac
    15481 ggcagcgccc cttccttgcg tctggcccgc gccttggcga agagatcccg aacgatgcgc
    15541 tcggactcgc ccaaccacat gttgagaatt tccggtccct tgatatggag gaacgcgccg
    15601 ctcgtcaccg gcggattttt cgtgtggccg tctgaagcag cctgcccgtg cgactcgcgc
    15661 accagctgcg acaaactggc cgccgccgcc tgcccgatca tggtcttgcc gcaaccgggg
    15721 ggaccgtaca gcagaaaccc tttcggctgc gtaaattggt acttcgtgaa ggtgtcggca
    15781 tggagcagcg gatactcgat cgccttacgg atggcatcaa tggcctgatg ctgcccgccg
    15841 atctgctccc acgtgacatt cggcacttcg tcaagcaggt gggtccgcgc ctgccgattt
    15901 tcaaactttt caatggcaat ccggtgtgtg ggttcgatgc ggacttcatc accggccttg
    15961 aggtccacgc ccagcaagtc gctggaccgc tgaagaatca gggcctgccg tcccatatcc
    16021 tgctcaaacc ggatacgtcc gtcaggcaag atctccgcaa ccttcaacac gggcccattg
    16081 cggtcatact ccagggcctt gatcaccgta tacgcctcgt tgaccagaat ctgagctccg
    16141 atcttgagct cctctgccgc cagccgcggg tcgatattgg cataatactc cgccccgcca
    16201 accacgatac gcgccacgcc ctcagccgga acctccacga gcaggcccac acggttggcc
    16261 ggcgccgtga gcttcgcgac gacctcattg agcttcttca attcggtatc ccgccgatcc
    16321 tgcgccaccg actgcgccgt cacgacatgg cgcaatttat aaagcagctt ctgacgaggg
    16381 tcgccgtcgg gaaaggccgc cagacattca tctatcagtt cgatgggatc ggcaggcgtg
    16441 gaggcatcgc gcgatggcga tggctgtccc ccatggccag gttgatccgc attccgatgg
    16501 tcactcatat cattcctccg gataatctca ctccggtcat cctaacaggt tgtcgagaag
    16561 ggccgccagc ggcgttctgg tggctctgac tccactcaat tgactggttg gagagtcgcc
    16621 gtcgcctgca tccggcgatt gccgcgcgcg aattcgatcg tgacctgatc accgacctta
    16681 tgttgttcca tggaatccat caggtcatcg atgctttcca ccggtttgcc atccacggcc
    16741 acaataatat cgcctaattc aatgcggccg ccggcggttt cccgcgcccc atgaagtccg
    16801 atgcgctccg ccgcactgcc gcgcccgacc ttgccgatga tgacgccttt gacaccccac
    16861 cgccgcgcca tggcatcagg caccagcgaa atgcccaatc ccggcctgat caactttcca
    16921 tgcttgatca attccggaac gatgcggttg atcgtatcga ccggcactgc aaacccgatc
    16981 ccggcaaacg ctccgctggg gctcacgatc tgcgtgttca caccaatcaa gcgcccgccg
    17041 ctgtccagca agggcccacc ggagttgccg gggttgatcg cggcatcggt ctgaatcacg
    17101 ccctcaatgg tccggttgct cattgatttg atggtgcggc ccaacgcgct gaccacaccg
    17161 gtcgtcaagg tgtgatcgag gccaaaggga ttcccgatcg ccagcacctt ttggcccacc
    17221 cgcagagact gagagttgcc gatggtgacc ggctggagtg tagcctcgga cgcctggatc
    17281 tgcagcaccg cgacatcatg atccggatcg gcaccgatga ccttcgcctt atattcggtc
    17341 cggtccgcca gtgtcaccgt gatggaatcc gccccgtaaa tcacgtggta gttggtcacg
    17401 atgtgccctt gccggctcca gacaaatccg gtcccggacc cttgcggcac ctcgaacaga
    17461 ttgaatgacc agggatcacg ctgcattgcc gtattggcga taaacaccac cgatctggta
    17521 gcccgatcga acacggcgat cgtcgcctgt tcctccggac tcagatcagt cggggccgca
    17581 acctgcgccg tgagcgaagg ggatgttgct tcggctgcga taccaccggc cggggaaaac
    17641 agccccgata gcacgctcga caacagcccc actccacagg cccatgcgac agacatgtgt
    17701 gtgcgcactt tcaccgctcg catctattca ccgatgatct gaagcaccaa ctctctggtt
    17761 cgtgcgcgcg tatcaaaatc cagcaccacg atctgttgcc acatgcccaa catcatggct
    17821 ccgtctgtga agggaatcgt gagggactgg ccctgcagct gtccgcgcag atggctatgc
    17881 ccattatcct cgcccgcatt gcgctcattg tgctgccagc gaggatcggc gggaataagg
    17941 cgctcccaga gaacctgggt atcggcacga atgccgggct catcttcgac gatcagcacg
    18001 gaggcggtcg tatgcttgat gaataccgtc acgatgccgg cccgcagtgg cgtttgctca
    18061 agcgccgcct ggacctgggc tgtgatattt tccatccggc tgtcgccctg catgggcact
    18121 cgaatcgtta ccgttttgac ggccatccgc tactccttac tagcctggcg taaaaatgcc
    18181 cgttctctct ttgataaagc ccggcccctc gcctgttcga gcacagcatc aaacacgcct
    18241 tccgccgaca actctcgcaa ggcctgaaca accggacgac tgatgatgcc ttctttctgc
    18301 agaccgttca ggtaggcgaa cgcttcggcc agatcttctc ggcgacgcac ataatagtca
    18361 cgaccacggc gctgattgct gaacgcttcg aactgttcgc acgcgacgag gatctcggcc
    18421 agctgccgaa cccaaggctg cgcaccggcg agcttttcag ggtagtaata atagagcatg
    18481 atccgcccca tccacggcgc ccaggccact cccaggtcac gcaattccgg ttttacgata
    18541 cggagtctcc cggccagacg gcgtgcatac cccagacgca tctccacctg ttcgcaagcc
    18601 catggatcga gggtgatgcc ggctgtctcc atcgccgttc gatagcgggc gacaaaggct
    18661 tcggtctcct tgccgtagcg tgtgtcagga tgggccgccc tccattcgcg cggcctggta
    18721 ggaatcccat gggcgcgggc ccaggaccag atgcggccga agagtgtccg atcgagtccg
    18781 gcacggccta agtcatgaag gagacaggcg atctgatact gcttcacccg cgccggtgcg
    18841 tggcccaacc gctgggccac cgccgcgcac atccgggccg tccgtacagc atgagggcag
    18901 tcatacccct tgatcattcg cccgggacga cgcggatcgg gatagtcgta aaatcgtagc
    18961 agcgtagagg cgaatgcccg cggcaccagc aagggaaggg aggtagtacg tgtacgtgcc
    19021 atgcaggagt gatgcaccgg gggcgacgat tgaccgcccg gcagaatatc gtccatgaaa
    19081 agaacgtgtc aagcagaccg tcgcgcagga caggatttcc tcgcactggc tgcggggctt
    19141 tgttattctg aatcggatgt cgatcgacca cgggagggaa ccatgagaat acctcggtcc
    19201 atgcagatcc tggcagccgg tgcgtgcctt ctgctggcgg acaccgccgc tcccgtgggt
    19261 gcgtccgatc aactggtgga tgtgaccggc aaggtcacgc cgttcgtcac gctgcgcagc
    19321 cgcgacaatt tttccagcga atatcgctac gacgtgacgg tgcgcaacag ttcacccgat
    19381 ctctttgtgg ctgacagtct catcctggtc ctggaccata tcaccaatac cgccggacag
    19441 gatcgtgaga acctgacggg cgaatccctt ttgacgagga tggaaatcat cgggcaggac
    19501 gggaccacag aggatggaaa accctatttc cgcattccgc aaggatcggg ccctgatctc
    19561 accccgaaca gtgaaagcct ccccgtcacc gtccgtatcc ggaaccggga ctacgtagcc
    19621 gtgttcaccc cttcattccg tgtcatgggc cagcgacgcg agccgccgaa acagaaatct
    19681 caagcagcag ctgccgcggc cgccgccgca ccgcataaag cgaccacgga caaactcatt
    19741 cagctcctgc tcaaaaaggg gttgatcacg gcggaagagg cacggaccct tctccaacca
    19801 tgagcgatca tgcttgcaag gcttgtgtcg ggacctggcc gagccagaat catttcatcg
    19861 ccgattgcgg cctcacgaag gcctatctcc acgacgacca attctttccc ggctggactg
    19921 tgctcgtctt caagcgccac gcgaccgaac tgttccatct ctcacgagag gaacgcagca
    19981 gcctcatcga agaagtcgct caggtatccg ccctccttgc cgaagaatgg cgggcggtga
    20041 agatcaacta cgagctgctc ggcaaccagc ttccgcacat ccattggcac ctgattcccc
    20101 gcctgtcgca ggacccggct ccgctcgagc cggtgtggcg catcgcgcat gaaccagtcc
    20161 ggcccgaacc ggatgccctc aagtccctgg tggcccgcct ccgggcaatc tggccggctc
    20221 accggcatca gccgccatcc agtgcctaaa cgacctccgg tcatgatgca gattccgcca
    20281 gggccgaacg gaagtagtcc ccgcaccttc ttccagacgt tgatccgcac tctgcctctg
    20341 cacgtactgg tcacaaccag catcgtggga ggggcggcac tcttttacag tctctccgtc
    20401 tcgaaccgtg gaacctggac catgctcgga agctggtccc tgacggctct gtatatcctc
    20461 atcgggctcg tgggcgggac tgtggccggc gtattggacg catcccggca gatggtggaa
    20521 cgcctggaac aagtcctccg tgactggctc catacgttgc ctgccctgaa ccggagcgca
    20581 gacaccaccg gccgagacgt gtccacggtt cggcaggaat acgaaaccct cgtcgaccac
    20641 tcggtcgcgc aagccgggcg gcggctgcgg ctaccgcagt ggcttgaacg gttgattcgc
    20701 gcgggagtgc ggggtctggt ggtcgatcgc ttcatcgcgt cctgtaccgc acggggaatc
    20761 catgtcgtcg cgcctcagga gtttcgaaac tggatgctcg ccgaaggcgt gagcctggga
    20821 ttcatgccgg tcctggatca attggcttgg tggcgctacc tcctcttagg agtgctcacg
    20881 ctcctggctg ccattgcgct gacgctggcc ttcctgacga cgtgagcgtg gttccgattg
    20941 atccacttcg tacatcggac tggtttcacc ccgatccggt cccggcgggt catgggcacg
    21001 gttccccgcg agatcccatg gaaatttccc gtcatctatg tgtatatagt cggatacctt
    21061 ttgcgtggag ccctgccagc acctatgttc ttgcaacgcg tctccgtcgg acggtggttt
    21121 ctcatagcga cagtggcatt gcctcttccg tctctgccgg ccggtgccca agaagagcct
    21181 tctcccgccg cggcaacagg aaccgtccaa gcagaaacac ccgtcaccga aacacccgct
    21241 gctccgcagg ctgaccaggc ccctcaagaa gcgatccgcg tggaaccgct tcccagcgaa
    21301 cctcccgcgc caccgccacc tccaccggtc gcgccgccgg ttcagcctcc tctgacacca
    21361 ctcgaaattc tggccagggc ccggcaagcg ctgcatatcg aaccggatgc tcaggagccc
    21421 cggctcaccc tcgggagaac gctcttccaa ctgggtgata cggatggcgc gatcgatgaa
    21481 taccggaccg cgctgcgctt ccatcccacg gtcgcgcagg cgcatctcga tctcggcacc
    21541 gcgctcatgg ccaagcagga ctggcgaacc gccatgacgg aattgcagga ggccgtccgc
    21601 cttgatccga cgctcgttca ggcgcactac agcatgggca cgatccaata cacacgcggc
    21661 aacatccagt cggccatcaa ggcctaccag gaagcgctgc ggttgaagcc tgattttgcc
    21721 gaagcccatt atcgcctggg cctcgtactc aaagttgcgg ggaaagacaa agacgcggcg
    21781 caggaacttg aagcggctgc gctggccggg ctgtccaaag cccaatactt tctcggcaat
    21841 gcctatcgct cgggacaggg tgtcgagaaa agccagatca tggccatcac ctggtgggca
    21901 caggcgttcg aacaggggct accggaagca gcgcaggcac tgacacaact ccggcgactg
    21961 gcagcggtca aaggcaacct gcagaccaaa cagtccaaag cggccgccga ggcgttcaag
    22021 aactattgcg atcaaatctg gctggacttc ccggacctcg accgcgacca acccacggag
    22081 accgtcggca cgacgttgct gaagcagggc cgcaccgccg aagccctccc ggtcctgctg
    22141 cgcgaagcct atgccctcaa cgagatggcc catgccacgc tggtgcaact gtacgaacag
    22201 ggactcgacg gccaactccc gccgcatggc caatggattt tgagttacct ggaatccact
    22261 gccgccgatg gagccgttcc ttcccgcctg gcactggccc gtatttatgg aaaagggctc
    22321 ggtctggctc ccgatcaggc caaggccaag agctatctga agggtttgcc gaaggatgac
    22381 gtcaaacgta tcctcgacga cgtcgccgct gaacctccca agccgtaacg agcagacaat
    22441 ttcagtatcg gttcgtccgt gacaccccat gcgaatcgcc acccgcctgt tcgtcaggaa
    22501 tatctggctg caagccgcgg tgatgacgct ggccgcgatc gtcctcagcg acatcctatg
    22561 gacgctggcc ccggccacgc gccaggcgtt ggaatgggca ccctatgaca cctggatgcg
    22621 ccttcgtgtc caagcggcgc ccgacccgaa cctgctcctg atcgtcagag accgggccag
    22681 cgatcaacag ttcggcaccg gttcgtggga tcgggcgctc cccgcaagac tcattaccgc
    22741 cctgcatgat gccggcgccg cctcgatcgg cctggatatt cctctcgagc ttcccagccc
    22801 gcccaatctc ggaggcgccg tcagtgacgc cctgctgatg gaagcggtga aatctgccgg
    22861 caccgtctcg tacccagcga tcggggtggc tgtctcagat caggaggcgg cggctctgtc
    22921 ctcccacttc acaccgggaa gaagcgccct gcacccgacg tttgaccccg atcgcgtggt
    22981 ccgccggttg ccgctgtatc atggcgacgg ccccggtgaa cttccggcct tgggattctc
    23041 attggccacg accttctggc aagtccaatc gaatcaactc gaaagacgat cggggcgaat
    23101 acatctgcac aacgcgcgag ttgcgggtgg atcgacaggc gatatcgaca tcccggttga
    23161 ccgctacggc cggctactca tcaacgttgc cggacatccg cttcccacgg tcttccctac
    23221 cctctctgtg ttggatctca cccgcatgat cgaccggaag gagaatgagg gcctcagcgc
    23281 cctggtcaac ggcaaagccg tgctgttcct cactcaaccg actcctcaac cggaacgcat
    23341 gatgccgacc ggcgaagagg caccggatat gctgctgcaa gcccatctgc tgcacacgct
    23401 gctgacgcaa gactggattc gggacctttc cccgctccaa cgggccatcg tcacattcgc
    23461 acttggcctc tccaccgcct ggggagtcct gcgctggacg gattggaaag gcccggttct
    23521 gggcgcgagc atgctggtgc tctatttctt cgtcagttgg gcaatgctgg cgaccgccca
    23581 ttgggtcttg ccgttcatca ttcctgtcac caccagcgtc atgattctcg tcgtcggcag
    23641 cctcctgggc cagatgatgg cgaccagacg cctggtgctg ttggaacagg acatgctgct
    23701 ggttcaacag aacctcgtgg ccgtccggga agccctgatc taccgggaaa cggccgtgga
    23761 aagtctggaa gaggatttag aatccgctcg cgccagcatg tctcattcag tctccaagga
    23821 agccgaatcg agcaggctcg cggcggagct ccaactcaag attgccgagg cccaggccca
    23881 ggaagatgcg acgcggcaac ggatggcggc gcttgaacaa cagctgcagg gcatgcgggc
    23941 tgcgacgatc acggcagagc cgctgggcaa cgtggaacag gacgcccttc gccgggaatg
    24001 tgagcaggca ggaatgatca ccaggaatcc ggcgatcctc acgatgtttc gcgacctgaa
    24061 gaaaggcgca cgcacgaccg tgaccgtcct gatcacaggc gaggcgggaa ccggcaaaga
    24121 actctgcgcc cgcgcgatcc accgtttgag tccacgagcg gcgaagccat tcattgccgt
    24181 caacatggcg gccatctcac cggaactgtt tgagagtgaa ctcttcgggc atgtccgcgg
    24241 cagttttacc ggcgcgcacg cggaccggaa aggctatttc gaactggcgc atctcgggac
    24301 gatttttctc gatgaaatcg gcgacctgcg gctggaccat caaagcaaac tgctgcgcgt
    24361 gctccaggat cgctccttct atcgagtcgg cgccacaacc cccaccacag tggacgttcg
    24421 cattgtcgcg gcgacgaaca aggatctcca gcgtggcgtt tcggaaggct ggtttcgtga
    24481 agacttgtat ttccgactca aaggcctcgt gctggacctg ccacccctgc gggagcgtcg
    24541 ggacgatatc cccctcattg cagaggccta tctccacgaa gctgcgaacc acaaccacca
    24601 atcatcgatg cgcctttcgg aagaggcagt ctcgacgctc atgcaacaat cctggccggg
    24661 caacgtgcgg gaattgcggc agtgcctgga gcaggccatg gcgctgtcgg acagtccgat
    24721 cctctccgcg gcggatctcc gcctgtccgg gccagcatca ccgaaaaccg gcacatccgg
    24781 tgccgcgccg ctgttgcccg atcctgcggg cgacctcgcc gtcttacaac aattgcgtca
    24841 acagcgcttc gacatgcaag cgacggcaaa ggctctcgga tgggatcgca gcacggtgac
    24901 ccaacgactc aaagggctct gctttcaagc gctcgtggat gccagcggag accaggccaa
    24961 agccgcagcc tcattggcgg gcgacccgtc tctcctgcgg acagtcgagc tgaaattgat
    25021 ggactactat ggccacctca tggagaccat cgctccgttc acaaacgtgg acgacgccct
    25081 gctcgactgc aagcggcgtt tcaaaaacct gcccgagcgc cacttcaaat ccgtcgaggt
    25141 cttggtgcgg cgacattttt ctgccgcatg atccgaacgc ctcactctgt cacaatgaga
    25201 ccgttcggtg cgtcggcgca gacacacagg cgtcagcagg gggtatttcc gtcaatagcg
    25261 cgcggagcca ggcatgactg aaccggcaca ggtaacgtgg acagaagaag gacagtcata
    25321 cgccgcgcat tggcgctcgg aatggggcgc gcctcccccg aaacgtgtgg tgatcgccga
    25381 cgaccgcatg actgccgatg cggcccatcg cttggcctgc gagggcaccg cattgctctg
    25441 gcgaggcgac ttccagaatg cgcgacaatt gctccttgcc atggccagac gcgcagaccg
    25501 ccatcctccg aaacccggcg cctctcccac ggagtccttt cactttcacc ggcagacgca
    25561 agcacgccgg gcccgcatcc tgggcatgct tctgatcact ctggaagatg attacgcaat
    25621 ccgcctccgt cgtgcgcccg atctccgtga agcctgtaca gaagcctacg gccccgcgac
    25681 ggctcgtgca ttggtctcac tgcgggaatt gctcggagtc atcggggcgc acgaatggcg
    25741 aaagaacggg atcgacatcc ctgccctggg cgaccgcatc catccgcact acggagtgtt
    25801 ttcgccgata cgcagcgagt atgtggcgct ggtggccgac gccccgctcc ctgcctgtac
    25861 gctggcgttt gatatcggca cgggtaccgg ggtactggcg gcaatcttgg cccgccgtgg
    25921 cgtcccgcgc atcattgcga ccgaccagga tcctcgcgcc ctcgcctgcg cccgtgagaa
    25981 tcttcaacga ctggagctaa gcgatcaggt cgaggtgcgg caagccaatc tgttccccga
    26041 gggcaaagcc cagctcgtcg tctgcaaccc gccatgggtt ccggcgcggc ccacctcgcc
    26101 ccttgagcag gcgatctacg atcccgagaa ccgcatgctg tacgggcttt tgaatgggct
    26161 agccgcccac ttggaaccgg gaggagaagg ctggctctta ctctccgatc tcgccgaaca
    26221 tctggcactg agatccagga aggaactcct cgccaccttc gagcaggcag ggctcgtcgt
    26281 cgcgggaaag accgatatcc ggcccactca tccacgcgcc tccgaacagg cggatcctct
    26341 ccgcgccgcc cgcgcagccg agttgacctc cttgtggcga ttgaagagcc gttgagcgcc
    26401 gaagtgaccc ggcgcgccgg agtgatcgcg ctacgggatg tagtgagcgg ggagcggcct
    26461 ggcggtctgg cattgacggg acgtgcaagg caaaccgggg aaccagcgct tcgcttgcaa
    26521 ttccttcaac cggcagagtt tgcgagtcgt atccccggtg gatgcgtaga tactcaacgt
    26581 aaattgatcc gcctgatcgc cgcttcccgc atcatcccaa cggtcaaaat aggtcgtccc
    26641 cttgtagcgg aacacgccgt acaactggta attggtttcc ccggcagccc gtccatcctg
    26701 gcgaggattt tgaagaacca gatccgtcaa ttcgtgatcg atgccgctga gattttccgt
    26761 gaggaccacg atgggagccg aataggcccg accaatcgat ccatcgtttc gagcgtggca
    26821 cagtgaggaa tggagcttga gaaccggttc tgccttccca tcattattga tatccacttg
    26881 cgcccaatag gcccagggct cgtccttggt gctgattctc cacagcgtat cctcaaatcg
    26941 gctttcgtcc ggaatgtttc tccctgactc gctgccggcg gtcaggaaat acaacacctg
    27001 ttccatggct tcgagatgtc ctgccacatc gaagggtttc catgccggag actccagatc
    27061 aaattgcgcg ctgtactccc gctcgcaagc aggatgcacc gtcattcgtt ccagattctg
    27121 tttgcaggct tcacagacgt cgattccctg accgagggtc aattgatagt ccgctgcttg
    27181 aaccagcgaa cccatgagga gccacgacat caacaaccac gaggacaacc tcttcatcga
    27241 acgattctcc ctgcctgaat tgagacatag gcgatcaccg gggattttgg tcgatcttac
    27301 agcagccctc atccctctca aataaattcc tggatcggcg ccggaccgat cacaagcaca
    27361 gccatacaac ccgattcccc ttcgagagag ctcatgctct ccaagagttg aacacctggg
    27421 tgcaaagacg acgtgatggg aagaaggctg gttgctgctg cagatcgagc gggatatccg
    27481 aaacttcgac caaggcacaa ccggctcggc cccttttagc aggcgacgct cgccagaggc
    27541 aagagtcagg tccatccgga tcaccgaagc atctccgcct cattccgctt gatccaccac
    27601 gcagtctcca tgcggggata gccccgcatc cccgctagtc cccgcatgaa tcgcggtggc
    27661 cacgcatcga tgcgtttcac ctaacccctt gcttcacaag ggtttcgtga tcgtccgcga
    27721 cgtcgattcg gctggcctcc gccttgctct atgcaccgcc caacggcggg cgttcggcag
    27781 gaaggaggtg accatcggca ggccggcccc gcaagacagc agcgggcaaa tcttcaccaa
    27841 caaccaacgg gccggagcga tccggctcaa caatgcaagg agcaggcacc atgcaaaaat
    27901 cccctatagc taccgtcagt gcaggaccag tcgcccccac cctcgtcggc ccggatcggg
    27961 acatgaagga cgtctatatc ctcagcggtc tcgtcgggtt cctcgccata gtcgtcgccg
    28021 ccttctggta ctactcccag gcggacgaag cggcccatag caatcgatcg tctgaagcgc
    28081 tcgatgtagc ccaggtgtcc caggttctca aggagtcgac cgagaaggtg acagggggag
    28141 ccgcgatgcc ggtcactcac gtcgaaacaa tccccctggc gagcaggagc acagagatcc
    28201 tgcacgacga catccagttt gaagtcggac gcaagggctt gaccgacgac gggaaggcct
    28261 ccctccagcg ccatgcagag ttcctgaaaa acgaaccgga ttggggcgtg ttgcttcaag
    28321 gttatacgga ccaacagggt tcgatgagct tcaacaagat tcttggcatg aagcgggccg
    28381 agacggtcaa gcagcagctc atctcgctgg gcgtgccgga atcggccatc cgcaccgtga
    28441 gcctgggtga agaaggcgcc ctgtgcatcg acaagagcga tgcctgccgg cagatgaacc
    28501 gacgggtgca tctggaaatg cgcaagatcg gccaggagca catggtgatt cctgccgtaa
    28561 tcaccgaacc agtcacggat tcattctccc tgaccaccga cctgtcgatg gaatcggctt
    28621 ccgccgaatc tgatagcgag aaccctactg gggtcggcgc gtcctccaac ggggcagaca
    28681 gtcctgtcga atcagcaacc ggcaactaac gtgacgtcga gctctccctg ggaaagacct
    28741 cgtctttccc agggaccagc aaccacgcac aacttccatc gaacaatatg aggatcgcat
    28801 gctgaatgaa tggttctttc tcccccgcgc tcgactacac agatgtgcac gcatcctgat
    28861 ggtctgcatg gcactcgcca cctctggggt gggaactcct cccacggacg ccggctcggc
    28921 cgaatcgccg gtcacgttga gtaccggagt cgttcccacc atgggcatca atgaggtcac
    28981 ggaagggacc ctgctgttcc gcacggacca ggccggtcga tttaccccgg ctcccgttct
    29041 caagaccgac gtgcacattg ccgtcaccgg caccatcgcc cgcgcgacgg tccgtcagga
    29101 atttacgaat cccagcacca gaaaaggcga ctggcttgag ggcatctacg tcttccctct
    29161 cccggaaaca gctgccgtcg atcacctgcg gatgcacgtc ggcgagcgga tcatcgaagg
    29221 acagattaaa gaacgggccg tagccaagaa ggtctatgaa caggccaaac agcagggaca
    29281 gcgaacgagc ctgttggatc aggaacgacc gaatatcttc acgacctccg tagccaatat
    29341 cggacccggc gagcacatca ccatcgaaat cgagtaccag gaaaccgtcc ggtatgacga
    29401 cgaacaattt cacctacgct ttcccatggc ggtgggacag cgctacatcc ccggcacgcc
    29461 ggtcattatc gaagggcagg cggccacagg atccggcacg atgctggata cagaccgcgt
    29521 ccccgacgcc tcgcgtatca ccccgccgat gcaatcaccc cgctatggcg cgatcaatcc
    29581 cctcagcctt tcattgacgc ttcatcctgg tttcccgatc gatacgatgg aatcatcgtt
    29641 tcatcccatc attagcatcc cggatgccga gggcggttat catgtcacgc tccgggccga
    29701 tgcagtgccg gccgatcgcg actttcagct cagctggcat cccgtggccc gcaccgagcc
    29761 gatcgcgacg gtctttactg agcagaagaa tgggcagacc tatgcgatgc tgatgctcgc
    29821 ccccccgacg cagcatgacg agaaggcgcc gcgcgttcct cgcgatctta cgttcatcat
    29881 cgatacctca gggtccatgg ccggcccgtc gatcgaccag gcgaaacgtg ccttggccgc
    29941 agcgctgcca cggctttcca tgcaggatcg cttcaacatc atccagttca accacacggt
    30001 ccgctcgctg tttgccgttc ctcagccggt gacgaccacg accatcaaac aggccatccg
    30061 ctatacggag cacctctcgg ccgatggcgg gaccgaagtg ctcccggccc tgcggcaagc
    30121 cctcaagagc ccgcaagaga gcgccagact gcaacagatc atcttattga ccgatggcca
    30181 ggtcgggaac gaagaggaat tgttcgagct gctgcaccaa cgtgtcggca ccaggcggct
    30241 gttcacgatc ggtatcggat ccacacccaa tagccacctg atgcggaaag ctgccgaatt
    30301 cggacgaggc accttcacct atatcggtaa agtggaggag gtcgaacaga agttggagcg
    30361 cctgttcaga aaactggaac gcccggtgct gacggacgtc tccgtcgatc aggccggatg
    30421 gtccgggctg gaacaatacc cggcaacagt cgccgacctg tatgaaggtg agccgattgt
    30481 gctcgccatg aaagccgatg ccgtgccgtc gcacgcaacc ttgcatgggc aggtagggag
    30541 ccgtccctgg tcgcttccgg tctcgttgaa ccaaaccgcg acgcaaggag ggctttccgt
    30601 ctattgggcg aggcaaaaaa tctcatccct gatggatgag gccaccaaag agggccgtgc
    30661 cgaggtcacc aaaacatccg tgatcgaagt ggcgttggcc caccaccttg tcagccaata
    30721 caccagtctg gtggcagtcg atgtgacacc ggctcgacca accgatgttc ctggagcgga
    30781 gccggggcag acaacgaacc tggccaacat gcagctgagc gcattgccga agaccgccac
    30841 cggctggcag ctgcaactcc tgctgggatt gtcagcactc gtgctggcgg cgctggtccg
    30901 cagactgcgg aacgaggtcg catgactgga acctggcacc ggtcacgccc ggtggtttgg
    30961 ttgatggcaa gccttctcgc catcggactg tggcaggtct gggaaggatc atggatgtat
    31021 gccaaggccg ggctcgcgca gcttctgctg cagcgggcct ggacccgttc tgtggaagga
    31081 gaggccatgc cgaagccctg gccctgggcc gacacctggc ccgtcgcacg cttgcggata
    31141 ccacgcctgt ctgtggatct gatcgtactg gccggcgccc atggacggac gttggccttc
    31201 gggccgggtc acgtcgggtc aagtgccctg ccgggacaga caggcacgat gatgcttgcc
    31261 gggcaccgag acacgcattt ccaatttttg aaggagctgc aaccggacga ccggctcgag
    31321 atcgtgggaa cggatggaac gacccgccgc tacagagtca ccgagcggca ggtcatggat
    31381 tcgcagcggg acagcattgc gacgagcgac gaacgcgcgg aacttatatt ggtcacctgt
    31441 tttcccttcg acgcgatcac gaccggcggc cccttgcggt tcgtggtgcg ggccgacgca
    31501 attcctcaag ccgatcgctc atgaggcgct ccggaagaac ttgagcacaa tggtcgccgc
    31561 acgccggccc catacctcat tcatgcgaac catggccctg gtttgctgtt cccgcagagg
    31621 ctgtcgctcc ggtcgagcgc gatggcttcc ggtagcggtg tagcaattgg tacagcacgg
    31681 gcagcactac cagaatgagt gccgctgagg tcagcatccc tcccaccacc actcttgcca
    31741 agggttgctg tgactgcgca ccgataccgg tggcaatggc tgcgggcagg agtccgatgg
    31801 cggcagccag ggaggtcatc aacaccggcc gcatccggac ttccgcgctt ttcatgatcg
    31861 cgtcgcgaat ctcataccct tcccgcaaca gttcctgcat ccggctgatc agaatcaggg
    31921 caccctgcac cgcgacaccg aagagcgaaa tgaatcccac tgccgccgaa atactgaaat
    31981 gcgtcccggt gactagcaaa gccaatacac cgccgaccag agagaagggc accgccgcga
    32041 ggacaaggaa ggcatcgcgc acattgttca gcacgaggta gacgagaaac agaatgagca
    32101 gcagagtaat gggcacgatc ttggcgagac gcgccttttc ctcctgcaac tgatcgaact
    32161 ccccatgcca ttcgatccgg taaccggcag ggagcggcac ctgttgctcg atgcgctctt
    32221 gcgcctctcg aaccgtgcct tccaggtcgc ggcctcgaac gctgaactta atcggaatat
    32281 agcgttcgtt attttcgcgg tacacgatga acgcccccgt ctgttccgtg atggaggcca
    32341 attgcttgag tggaatcctg gccccatcgg gagtactcac gacgatattc ccgatcgatt
    32401 cgatatcgcg ccggaactca gggagaaacc gcaccaccag atcaaaccag cgttcccctt
    32461 catagatctg cgtgaccgcc tggccgccaa tggccgcctg caccacatcg ttgacgtcgc
    32521 ccactttgag gccgtagcgc gcgcattcct cgcgattgac ctcaatgacc aggttgggtt
    32581 gccccaacag atggagcacg cccagatcct tcacgccatg cacatccttc atgaccagct
    32641 cgatctgtgc ggccagttgc tccaacgtct ggagatcgct gccgtacagt ttgacggcgt
    32701 tctctccctt cacacctgac atcgcctcct gcacattgtc ttgaatcgcc tgggagaagt
    32761 tgaaggtcac accgggaatc acggcgaggc gttgctcgat ttgatcgacc agggcttttt
    32821 tggtcggcac atccgcccgc cactccttga acggcttcaa cgtcacaagg aattcggcat
    32881 tgaagaaact ggtgggatcg gtgccgtcgt cgggtcggcc taattgcgag gccgcactga
    32941 tgacctccgg aaactgcctg aagatgccgc gaatgtccgt caccaggcga gccgcctgtt
    33001 cgaacgaaat gtccacgggg aaggtggtgc gcatccagat attaccttct tccagggccg
    33061 gcatgaactc accgccgatg aagggcacgg ccgccatggc gaggactaac gtcacgatcg
    33121 cgatgccgat caccaaccat tcgcgttgaa gcccccaatt cagcaaagcc atatagcggc
    33181 ggctcaagaa ttccaccacc gctgtgtcac gctctttgat cggtccagtc agcagaagcg
    33241 agcacaatgc cggcgccagg gtgaacgcca tcagcaatgc accggtcaac gcaaagccat
    33301 aggtcaggga catcggcgca aatacttttc ccggcacccc tgtcatggtg aagagaggca
    33361 aaaacgcgat gacgatgatc gtcgtggcga agaaaatcgg acgcccgacc tcccgcgccg
    33421 cccgcatgac atgcatcggc accgtcacgc cggccgaaga tttatgcgca agatgataga
    33481 aaatactttc gaccatgatg agggtcgaat cgacgatgat cccaaagtcg accgccccca
    33541 acgagatcaa attggccgac tggccgctga ggaccatcat cccgaatgtg aagagcaggg
    33601 ccacggggac ggtcagcgcg acgatcatcg cggtccggaa atgtcccagg aacacataga
    33661 gaatgatcga gaccagcacg acacccgcga tcaaaatatc gatgacggtc tcaatggtgg
    33721 tatggatcag taccgtgcgg tcgtaaaagg tcttgatcgc cacaccctgc ggcaactttc
    33781 tggcgttgag ctcctgcacc ttctcgtgaa ccttgtcgag caccggcaag gccttggcgc
    33841 ctcgctggag caacacgacg ccttcgacaa catcgtcgcg atcatcgatg ccgactttgc
    33901 ccaggcgcac acgggaaccg atgctggtct tcccgagatt gcgcacgaac accggcgtgc
    33961 cttccttctg cgtcacgacc gtgttggaaa tgtcctcgag tttcttgatc aggcccatgc
    34021 cgcgaatatt gaagctttgc tcccccatcg agaggtagtt gccgcctaca tcggtgttgc
    34081 tcttggccag gccatccatg acctggtcaa gcgtcacgtt gtaactcatg agctgcccgg
    34141 gatctaactc gacgtgatac tcct
//
LOCUS       DAOQEW010000017        32703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_291_length_32703_cov_9.160653, whole genome shotgun sequence.
ACCESSION   DAOQEW010000017 DAOQEW010000000
VERSION     DAOQEW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 32703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32703
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_32703_cov_9.160653"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..508
                     /gene="ndhC"
                     /locus_tag="PLS35_03410"
     CDS             125..508
                     /gene="ndhC"
                     /locus_tag="PLS35_03410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNI17732.1"
                     /translation="MAGSELLLEYLSRYFPVLVFVFIALAFGAGTLIISYFVQPKYPE
                     PEKLSTYECGSEPFSDARMPFPVRYYIFAMLFVIFDVEVIFLYPWAIVFNKIGLIGLI
                     EMLIFIGLFLVAYVYAWRKGALEWD"
     gene            499..1026
                     /locus_tag="PLS35_03415"
     CDS             499..1026
                     /locus_tag="PLS35_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNI17733.1"
                     /translation="MGLIQLGGHDKDGSPDVITLTVEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDMDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VIAMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQERIMQKR
                     VFLSQPKEVKDALKV"
     gene            1043..1540
                     /locus_tag="PLS35_03420"
     CDS             1043..1540
                     /locus_tag="PLS35_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNI17734.1"
                     /translation="MSQLAQRIEDTFPGACTKVVEWRGDLAVTVKPETLHDVAKFLRD
                     DPALRFDYIVHVSSVDWPDDEDRFEVVYEVYSIRTRQRIRLKTRVPESDCIVDSLTDV
                     WKGADFMEREVYDMMGIRFRNHPDLRRILMPDEYDEGYPLRKDFPLRGKGWRDTFEFL
                     DEPTR"
     gene            1569..2816
                     /gene="nuoD"
                     /locus_tag="PLS35_03425"
     CDS             1569..2816
                     /gene="nuoD"
                     /locus_tag="PLS35_03425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNI17735.1"
                     /translation="MAQFEDQRTTVYKIDPEHPESETLPTLRTEELLLNMGPQHPSTH
                     GVLKVILELEGERLVKSTPVMGFLHRGVEKLAEEGTYHQFIPHTDRLDYVCAMYNNFA
                     YCRAVEKLMNITVPDRAEYLRTIVAEIQRIIGHQFWLGTQALDIGAMTVFFYCFRDRE
                     ILLDWFDELCGARLTTSWYRIGGVERDFTPSLFAKLKQFLDYFPPKIDEYVIFLEKNR
                     IWLARTKGVAVISAEDALSFGLSGPTLRGSGVDFDLRKYEPYSAYPKCEFSVPVGKNG
                     DTYDRYWIRVQELYESVKIIRQCLEQIQDGPIMADVPSVTLPPKERVFTNLESMIQQF
                     KLFSQGFNAPPGEIYCGTEAHKGELGFYIVSTGGGKPYRLKIRAPSFIHMGAFDHMSK
                     GYMISDAVTIFGTYDIVMGECDR"
     gene            2902..5595
                     /locus_tag="PLS35_03430"
     CDS             2902..5595
                     /locus_tag="PLS35_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNI17736.1"
                     /translation="MKPATNPDVEAATIELSIDGRTVSAKDGVSLYDVIASTGKIIPA
                     MCYHYTFDPFGSCGMCLVMQEGKKAPVRSCTAKAAAGMVIRTDGDDLFAARKKAVEKH
                     LSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKFIPEDTRSLVLDFNMNRCIAC
                     AECINVCKDVLMIDALQFMKKGGFNQVVAKGDQPLACEFCGDCLAVCPVGAITNKYSK
                     YLYKPWQMRKTTTTCNYCGDGCQMHLETKDTEVVRVTSPLSWKNKWGDRSETAKGHGG
                     LCVRGRFGFQYIDSQQRLRQPLVRTGDTLKETPWLDAMQTVIDQLSDIHRKHGPESIA
                     GLITARCTNEELYLFQKLMRGGLRTNQLDSSARYGHLNYVHAVRHAVGIGRPLNDWED
                     LTKAKAVLVIGSNITETNPLTAVRIKEAIRVYHAQVIVVDSSNTNIAQLASHPLLVKP
                     GTEALLIDGLVKAVIEQDLVDETSTTQHPQAFAALKQAVAQISLDEVSRTTGISVDQI
                     REVAAIFAEAPRAVALCAEGIVRRPNGYENVLKLVDLAWVTGKLGQPGCGVTTVTEEV
                     NEQGAIDMGVAPEFLPGQARFDDPGARERFGKAWEVTLPAIGTGANLLEIFTRIQKGQ
                     IKALYVIGENPLATLPASMDVKGALDKLQLLIVQDPFLTETGRMAHVVLPAATYAEKD
                     GTFTNLEGKVLRVRQALDQVGESLPDWHIMTALANGLGYEWPYQSPQDVQNEIMKLLP
                     GYYNLGQPRRIAPSADAYLSNGYSSEVVTRYRRRQAMDDTSRPFTLVMGQVLYHSGKM
                     STQASGLINIEPNNGRIRMNPADVEKLGLTEQSTVRLSSQQASVQAGVKADPDVQSGS
                     CFFPEHFNEPPIKDLMTTEVDAVTGVPYFKSTRVKIEKVGA"
     gene            5640..6227
                     /gene="nuoI"
                     /locus_tag="PLS35_03435"
     CDS             5640..6227
                     /gene="nuoI"
                     /locus_tag="PLS35_03435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNI17737.1"
                     /translation="MSVGALTKKVLQAALFYEIWDAMKVTFKHMFHRPITFQYPREQR
                     TIPDGHRGALGLLRYSDGRDRCVGCDLCEAACPSRCIKVISQEDPERPLQRFASEFYI
                     DITKCVFCGYCVEACPVNALAMTKMYEYSTHDKRTLLFDKKRLYEVGERHLEDAKKYL
                     YTHNQEKNSDESREYRYYFPQSVLKPTQSQPKHLS"
     gene            6239..6763
                     /locus_tag="PLS35_03440"
     CDS             6239..6763
                     /locus_tag="PLS35_03440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNI17738.1"
                     /translation="MAFVFFAYFAVVSIVAGILTVALKNPVQCGLALLALLLHVSGLF
                     VMLNAEFLWAVQVIVYAGAILVLYLFVLMLLNLKTDDRYFHARTPYLLAPAVIGLIYL
                     LFLLIRSPFSGSRGDASTAAVLLNGDAHAVGIKMFSDYLLQFEIIGVFLTGAIVGAIV
                     LAKAPKSIETRRDA"
     gene            6760..7062
                     /gene="nuoK"
                     /locus_tag="PLS35_03445"
     CDS             6760..7062
                     /gene="nuoK"
                     /locus_tag="PLS35_03445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNI17739.1"
                     /translation="MIPLSAYVAVSAILFTTGLLGVLIRRNFIIVLMSVEIMLNAATI
                     NLVAFSHYLESMQGQIVALFIIAIAAGEAAIGLAIIIVVFRGKISTNVDEMNLLKW"
     gene            7064..9067
                     /gene="nuoL"
                     /locus_tag="PLS35_03450"
     CDS             7064..9067
                     /gene="nuoL"
                     /locus_tag="PLS35_03450"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNI17740.1"
                     /translation="MSDSIDLIVKLIPLFPLMAVIVNGLFGHRYSHDLAHRFAWGSVL
                     MSFLCVLAVFTETLRTGAAREVIAYKWIFGGDLTINLAYLIDPLTCIMLLVITGVGFL
                     IHVYSVGYMHGEAGFTRFFVYMNLFMVSMLLLVMGNNYVVLFIGWEGVGLCSYLLIGY
                     YYDKVSAAKAATKAFVVNRIGDAGFLLAIFLVFVNFRTLDYTQVMQNAAQLSPEMATA
                     IALCLLVGAVGKSAQLPLYTWLPDAMEGPTPVSALIHAATMVTAGVYMIVRNHAIFDL
                     APMAMTTVAWIGGLTALFAATIGLVQTDIKRVLAYSTVSQLGYMFLGCGLGAYTAAVF
                     HLMTHAFFKALLFLSAGSVIHALSGEQDIRKMGALKSKIPWTHTLFLIGTIAIAGIPP
                     LAGFWSKDEIMGHAFVHHHYLLYGMAAVGAFLTSFYMFRLTYLTFYGTSRLDHHTAEH
                     VHESPMVMIGPLVVLATLSVIGGFPGVPPENGWFHHFLHSVAGVAGEEHATPPALMFG
                     LMGTATVIALLGWGLAHYFYSGPSTAAAALADRMPGAYTTLLNKYYVDELYDTLFVEP
                     TKQLGRLCDWFDRTIIDGLVRGIDRGTDLSSAAITWTEKHVVYAGLNIIGYANHLLAR
                     SWRRLQTGMVHQYAAIIVAGLFILVHLILLIWTGGGSTGYSAR"
     gene            9091..10650
                     /locus_tag="PLS35_03455"
     CDS             9091..10650
                     /locus_tag="PLS35_03455"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNI17741.1"
                     /translation="MLEEFSFGFPILSYLIFLPLVGAAVLWLIDDEDLLKSTTLGITL
                     VELALACLVLVRFVPDSAAMQFAEHARWMPALGIGYHLAVDGISVLFVGLTAFLTVLV
                     VIYSWDTIRNQVRLYFMALLALETTTIGIFVSIDLILFFVFWELMLIPSYFLIKLWGG
                     GADRHYAALKYVLYTLLGSVFMLVGIALLDINYHQWAVSHHMDHVYSFDLLELLSVPI
                     PLSQQILIFWLMFMGFAFKAPVFPFHTWLPDALVEGPIGMAVMLAGMKLGTYGFLRFT
                     FPLVPDASKSEGVVGIVMVLALSAILYGAVVALVQSDFKRLLAFSSISHLGFVVVGLF
                     ALNFQGLQGSLLTMINLGFSTAGLFFMAGFLSTRQQSSHLSSFGGFAKQAPLLATFLL
                     LIGMASIGLPGTNGFVGEFLILLGAFKAKWWFGAVAVLGVIFGAAYFLWYYERAMLGP
                     LSKHVSAAVKDLHMREITIALSLSIMILWIGLYPSPFLRMMNGSIQALVDRLDRAKVA
                     SVDTVIHVPAK"
     gene            10654..12309
                     /locus_tag="PLS35_03460"
     CDS             10654..12309
                     /locus_tag="PLS35_03460"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNI17742.1"
                     /translation="MAEYTLLIILFAPFLGALGLIFVSNRQVSLVRGIAAGSAFVSLA
                     ASVYLFYAYDSVKGGYQFIQRIEWSRQLGISLHLGVDGIGTPLVLASGILLFAGIFVS
                     WHIKDRMKEFYIWILILAAATIGVFMSLDLFFLYFFYEMSVIPMYLLLGMWGSHTKKY
                     LEMTDPEGLKQRDSVGFIFNFGANSKEYAAMKLVLFLSAFAVAALMGILLIYKFSGLN
                     TFDILILREKAHFSGPLATLIWLLIFFGFASIAPIWPLHSWSPVGHAAAPAATSMLHA
                     GVLMKLGHFSIIRVAFEILPETTRELMPIAAVLCIFSIIYGGLVAYYAKDTKYVIGYS
                     SSSHMGYVFLGMAALDYISLSGAVIYMFAHAMATGMLFAMAGWVYDQTHTRDIPSLGG
                     LSNRMPFISAAFVIGCMASIGMPGTVNFIAEVMIIVGSWNKYPFQVVVAVLGIVLTMA
                     YLFKMMRGLFYGSMAEKYSHSHDAVAVVDRMPLLIMITVSISFGIFPGHLYSVVRSGV
                     DPLIARITKVVPVAELPMNIPQASSHTMPVPVPGEAIAKVTSR"
     gene            12306..13802
                     /locus_tag="PLS35_03465"
     CDS             12306..13802
                     /locus_tag="PLS35_03465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNI17743.1"
                     /translation="MTFSLTLSASDLLLLLPELFLTLWLCVVLAVDFSFKRIVQEQLA
                     YLTILGVIITLACLAWFDMTGITGTLFGKMFVVDRMAIFFKVMILLATILVILLSIDY
                     VHRFSLFRGEYYFLVAMSALGMMFMASANDLLSLFVTLEFATFGFYVLVSYLRDDMAS
                     NEAGLKFFILGVFAAGLLAYGISLVYGETGKLVFSDMSGANPTTGLVIGFLLIFAALG
                     FKIGAVPFHSWIPDTYHGAPTPVTAFLSIAPKVAAFAILLRLFLVALATFKPAWALLI
                     VAASILSMTYGNIVAIAQRNIKRLLAYSGIAQVGNVLIGLAAGTKMGTDSILFYLLTY
                     LFANLGAFAVIMAISNSVGSDEIDDYRGLNRRSPFLAFAMLIFLLSLAGVPPLAGFIG
                     KLYIFVAAIKEGLYILITVGLVNIVVSMYYYLIVVKKMYINEPLDSSPIKTTGPLRAV
                     IYVGLAGTLVIGIYPQPFLDWAVAATLMFSNLIGPAASLTPPVTPFGG"
     gene            13961..15457
                     /locus_tag="PLS35_03470"
     CDS             13961..15457
                     /locus_tag="PLS35_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNI17744.1"
                     /translation="MNEPNRHQPVDVGLDSAFPPASPAEEKSSGRVSFLSPLLHTFQK
                     RSIEQRVLAGFGLVFAGILVISAISYRNMTVLIRNGDLDKRSHEFIQFLGATGEAIDD
                     AENGHRRFLVTGDESYLAAYNTLRDRAPEYIRYLRELTDPGSTQRGHVSDLEHLINQQ
                     LQEEHAAIELRAKTGFDSVRGMALTGVAKTALDSARRLQAQMDQEETKALTQRVVEST
                     TTTRSSIILLTIGALLLFVLLAAVYYLIRHDITERRRIADELHRRGELLEAANKELEA
                     FSYSVSHDLRAPLRHIDGYASLLAKAASISLDDKAKRYLQTISESATRMGQLIDDLLV
                     FSRMGRQEMLRGTVNLNQLIAALRHDLRHDLQDRAISWTIAQLPEVTGDAAMLRQVFM
                     NLMTNAIKFTGTRPQATIEIGSLDNGPDEAVLFVRDNGVGFDMRYANKLFGVFQRLHR
                     ADEFEGTGIGLANVRRIIHRHGGKTWAEGALGEGATFYVSLPRVRPLA"
     gene            15454..15921
                     /locus_tag="PLS35_03475"
     CDS             15454..15921
                     /locus_tag="PLS35_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17745.1"
                     /translation="MIASKPILLAEDNPRDAELALAAMEEHHLADKVILCHDGAEVLD
                     YLYCRGHFKTRLQGNPAVVLLDLKMPKVDGLEVLRTIKNDAALRPIPVVMLTSSREER
                     DLAESYALGANAYVVKPVEFHQFLKAVKELGVFWGMINEPPPEGASRSAGRSS"
     gene            15939..17582
                     /locus_tag="PLS35_03480"
     CDS             15939..17582
                     /locus_tag="PLS35_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17746.1"
                     /translation="MMTQPLRFIHLESNPADADLIDSTLREAGILCQPKHVHTREDFL
                     AALRQESFSLILADTTVPGFDGAAALGLARTLHPDVPFLFVSATQGEEFAIDMMQRGA
                     TDYIFKQRLGRLVPSIKRTLRELDERLERRRAEDALRMSEKQFRQAQKMEAVGRLAGG
                     LAHDFNNLLTVIMGHSQVLLGELPAGNPIRVKIEEMQKAGERAASLIRQLMAFSRKQP
                     VEPQVLPLNAILANVEGMLRRLIGEDIQLVIRPDPHHGHVKADPGQLEQVLMNLVVNA
                     RDAMPNGGLLAIETSQTELARTPMHHLHPLPLGHYVKLTVTDTGCGMDAEVLAHLFEP
                     FFTTKEEHKGTGLGLSTVFGIVTTCGGGIDVWSQVGHGTTFDLYFPRANPQTSAARSE
                     KPQSRPREGSETILLVEDDGGVRDLIRHELVKTGYQVIEAKNGVEACLTATQQSYHVD
                     LLLTDVVMPGMNGRELAQHLSVIKPNLRVLFMSGYLDDICVNSGMDPHRTTFLQKPFT
                     PDILLRTVRALLDSSSPATGQTQHPFSPPDQAPRTARAS"
     gene            17881..18636
                     /locus_tag="PLS35_03485"
     CDS             17881..18636
                     /locus_tag="PLS35_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNI17747.1"
                     /translation="MEACPQVPSLLMLQIDPERYRFSIHQFRDEGLRSPISIHDWQQR
                     TDAYVLFNAGLFREDYSYLGVLLKEGRSLGTKKHHSWQGLFAAEPTDGTLKKARVLDL
                     AYEGFTEDPPLYREAAQSLMLFDRTGKLRVKDSGKRAFQTVVAEDGQGAILVIKTVDI
                     VSLHHLADCLHRQLPALQQAMAMDGGASSDVIASPDLLHAAQETSSQAGWRSLLAGTT
                     GVHIPLPTVIGITPRTPSRSTPAHGPSQSTRAH"
     gene            complement(18638..19069)
                     /locus_tag="PLS35_03490"
     CDS             complement(18638..19069)
                     /locus_tag="PLS35_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI17748.1"
                     /translation="MRYLTSIMCGLCLCGLLFLLDVPQPAVAYHSYVLSVQQLRAGLM
                     KASSTNAKGFVLVDVRSPEEHASGMIPGTDLNIDFREMKARHRELGAKLEDHIVVYCQ
                     SGHRSNIAAETLADLGYTHVYNVVGSMNAWTEAGFPVSPGR"
     gene            19135..19446
                     /locus_tag="PLS35_03495"
     CDS             19135..19446
                     /locus_tag="PLS35_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17749.1"
                     /translation="MKRWSSCTVLTVSLFLSSCGEGALLVKDSADGGVVVYPYRAEQG
                     TLLSSFRKEAVRLMEQKCPGGYAIVREGETKGRLRQASPVAGSSDVVEERRWGIQFQC
                     K"
     gene            complement(19447..20022)
                     /gene="pyrE"
                     /locus_tag="PLS35_03500"
     CDS             complement(19447..20022)
                     /gene="pyrE"
                     /locus_tag="PLS35_03500"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNI17750.1"
                     /translation="MLQDELIAAFHKTQSFKWDPKGGFKLASGLLSPFYVDCRTLMAF
                     PHARHLVAKKAWEVIKDLQIDCLGGLEIGAISIATSISDYAYSAAPRREWRTFFVRKQ
                     AKDHGLGRLVEGVVRTGDRALIVDDVLTSGGSVVKAIVAAREAGLEITDALVIVDRKE
                     QDGRARVEQTGVKVMSLLTIDDLMKGGPIPI"
     gene            complement(20208..20717)
                     /locus_tag="PLS35_03505"
     CDS             complement(20208..20717)
                     /locus_tag="PLS35_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HNI17751.1"
                     /translation="MATVRKGVILVGHGGIPRGCPQDMVTKLKRLEAQRRAAKQPASA
                     EEMELDAKIRQWPRNAETDPYQAGLEAVAAQLRAKLGDVLFAVAYNEFCAPTLEESVE
                     QLAKQGATHITVATTMFTPGGSHSEVEIPEILEHLRPQYPGVELRYAWPFDLGMIAGT
                     LAEQVRRFS"
     gene            complement(20756..21124)
                     /locus_tag="PLS35_03510"
     CDS             complement(20756..21124)
                     /locus_tag="PLS35_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17752.1"
                     /translation="MPHVVVEGAGDLQSLYQNFAPMLQRADQEILKVQEFYLAKSGRE
                     ALLDAVVIEQGVARSFFIQLKQHDTTITVRLLPATDPEKTAAVKRVMALVAKFIRTVC
                     PASRYGKTNLQEFLEASVAK"
     gene            complement(21135..22088)
                     /locus_tag="PLS35_03515"
     CDS             complement(21135..22088)
                     /locus_tag="PLS35_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNI17753.1"
                     /translation="MTAATLRLGISRCLLGEEVRFDGGHKRDAFLTDVLGRYVEWVPV
                     CPEVEAGLGTPREAMRLVGNPQQPRLVTLKSGQDHTRALEVMADRRLDELAGADLSGF
                     VFKKDSPSCGVERVRTYNEHGMPGRKGVGLFARAFLGRFPLIPVEEEGRLCDPLLREN
                     FIERVFCYRRWRDLMNSGATRQAVVRFHTVHKYLLLAHSAPHYRQLGRLVARVSEYRP
                     KDLALQYGELFMQALAVRATVRKHVNVLHHILGHFKERLDAQEKAELLGVIGDYHQGL
                     TPLIVPLTLIKHYVQLFDVEYIREQVYLNPHPKELMLRNHV"
     gene            complement(22085..23524)
                     /locus_tag="PLS35_03520"
     CDS             complement(22085..23524)
                     /locus_tag="PLS35_03520"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNI17754.1"
                     /translation="MRGLVWFRRDLRLHDNPALSAACKECKEIVPLFVFDDPLLASHV
                     FGSVCVGFMLGCLEDLRRSLAEQGVTLVWRMGEPIDTVLRTVQECAVDAVYWNRDYEP
                     AAVARDRTVQQRLAQQGRLVRTFKDHVVFEAEEVRGLTGDPFQRYSAYRDRWWTQWRS
                     AAPPLLSVPSFPAASTAMKVSAWPTVQELGYESVPMWIEPGEQAARARLQWFLKKPVH
                     SYVSGRNLPAQDGTSKLSPHLRFGTISTRTMVHAALQTLSKGGQVSRPDVFTWMDELV
                     WREFFQQVLMAFPHVTEGPFKVKPGLPAPRPAGPERDRLFAAWCEGRTGYPIVDAGMR
                     QLNQTGWMHNRVRMVVASFLVKDLRIDWQSGERYFMQHLVDGDVAANNGNWQWCASTG
                     TDAMQGYRIFNPTLQSEKFDPDGHYLRCYVPELTSVPTKWIHEPQLMPQEEQIRVGCR
                     IGSDYPEPIVNHRQARQEYLDLGKQKVST"
     gene            complement(23521..23952)
                     /locus_tag="PLS35_03525"
     CDS             complement(23521..23952)
                     /locus_tag="PLS35_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17755.1"
                     /translation="MIQQTSQRGIDIKLFGALFLLVGLLDVLIIEWFPAYALKLFGVT
                     ITGPMAYVVKLHSPAAHFLIGYGFIFLRPWAWGLAMAYGGFGLTSELMNQWQFGFHQL
                     RTGFMATTALFLCYVAWRRVLFVDPLSPDTRLASSSREVHL"
     gene            complement(24002..24526)
                     /locus_tag="PLS35_03530"
     CDS             complement(24002..24526)
                     /locus_tag="PLS35_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17756.1"
                     /translation="MKRMIALLGCFVMISGVCLTEVRANPTPASTLSGVEKTGLYLLA
                     GDYRRALESCEQALQERPSVEAYVRLTYVYQAIDAYLEQLSKDESWMAVEHLYLNLAY
                     RHTEDLVDPPGGLARMAKEMIQTSVRQQSDVSAAMAVRLNKVASDRLWQEQTQWRQAH
                     PTTWWQAFPDAWVQ"
     gene            complement(24534..25019)
                     /locus_tag="PLS35_03535"
     CDS             complement(24534..25019)
                     /locus_tag="PLS35_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNI17757.1"
                     /translation="MMRKHTYRVAWMTLGVLSLCLSQWPEGTGKSWAAEGHEKSGTPG
                     GSYGLEVTIPDGVIGVSLHVGAERVGDTAVLYIAQVLPDGPAEKAGLKSGDEITTVDG
                     AVVTGKTYEQVALMVRGEPGTIVRLGIKAETAVREVAVTRVAGQTLYKGQMGSHGGSA
                     K"
     gene            complement(25044..25520)
                     /locus_tag="PLS35_03540"
     CDS             complement(25044..25520)
                     /locus_tag="PLS35_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNI17758.1"
                     /translation="MSLLVESIMQYLHGLAAAILVGKVVLLSFVVAPVLAKQLAPEQF
                     GKVVRQLFPAYYLLGMGAAAGGLVSVTALLVIRGASPGLSAAGGIWLIIFLAEAYCRS
                     PLTPQSNAMRDQLKEQESRGSVDPVLKASWERLHQRSLYLNSLVLLGGLCLVGLAS"
     gene            complement(25517..26434)
                     /locus_tag="PLS35_03545"
     CDS             complement(25517..26434)
                     /locus_tag="PLS35_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNI17759.1"
                     /translation="MNIVVTGGTGFIGHALVEELSHAGHDVVVLSRRPHSPTQSKVRY
                     VEWDARSVGPWQDAVAAADAVVNLAGEPIAEGRWTSARKRILFESRILATRSLVDAIA
                     TRSRPLPVLISASGIGYYGLQDDRVLDERSPLGEGFLAQLSAAWEAEALRAGQFGTRV
                     VLLRTGMVLERDGGALPKMMLPFRLFAGGPVLPGTQWVSWIHRADLIGLMQWALHSPE
                     VSGPVNAVAPGPETMKAFCGILGKVLHRPSWLPVPGLALRLGLGELGTLMTTGQRVDP
                     AKAQAGGYRFRYPTLEPALRAIMTKEQRA"
     gene            complement(26465..26737)
                     /locus_tag="PLS35_03550"
     CDS             complement(26465..26737)
                     /locus_tag="PLS35_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17760.1"
                     /translation="MTQHYIDLLDDTGQIDITAWHCAMCGEVLDPVILKNRHSPPPNL
                     LYGTKERKFSQRVTAVSNGGASKKSSHREAAPDKGKDSETGADRAP"
     gene            complement(26914..27045)
                     /locus_tag="PLS35_03555"
     CDS             complement(26914..27045)
                     /locus_tag="PLS35_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17761.1"
                     /translation="MTQEMILGMAAFVVSIGAVLYVRLRRPAYVMVRRSSREHPRVR"
     gene            complement(27229..28092)
                     /locus_tag="PLS35_03560"
     CDS             complement(27229..28092)
                     /locus_tag="PLS35_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI17762.1"
                     /translation="MTQGLTRRRTRWGYAGLLAAASTLALSPVFATPAVKELDPVLMV
                     TIPAGPFLMGTQSPKGRADEWPQRTVQVDAFAMDEVEVTNERYMTFVTTTGHRSPPNP
                     YGTGVLVSAKGIEQLPVVQVTWYDAKAYCAWAKKRLPTEAEWEKAARGTDGRTFPWGE
                     DTPTSKRANYDREWVEDKTLHPVGALPAGDSPYGIKDMSGNAREWVQDWYDPDYYANA
                     PTKNPQGPDKGIVRVIRGGSWHSPHSDITSTARGRGGFALQTHGTGFRCVRSLDGQAH
                     QDASGAEPKAR"
     gene            complement(28089..28457)
                     /locus_tag="PLS35_03565"
     CDS             complement(28089..28457)
                     /locus_tag="PLS35_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17763.1"
                     /translation="MRHRTIVTIIGGLAALLSAMELQAGPIDASRHTHPEKVQLVHEA
                     EHSVDHAWEVYHRAALGGTVASPDLQAQIEQHLHEARTLVTKAQEAADRGDAGAVEKL
                     VAQIKTHTERAIEGSKEQKK"
     gene            complement(28511..29569)
                     /locus_tag="PLS35_03570"
     CDS             complement(28511..29569)
                     /locus_tag="PLS35_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI17764.1"
                     /translation="MTVMGTVVRAGLVMGIIAGASVAQAHEAEIPKDMVYVGQGPSIM
                     GLDRTEAAAPTRRLSLYDKRMSMPWSAEAFHDEGPAHTVFLDSYLIDKYEVSNKDYGE
                     FIKATGHPAPAYWDDPRLNKPQQPVVGVNWIDAKTYCEYRGKRLPTEAEWEKAARGPN
                     GNLYPWGNDFDSNKANYDRHHNATQPVDAHPEGASYYGVYNMAGNVFEWVGDWYDPKY
                     YGRLETMVNPTGPEKPLWLGGTGTYVDRLTVGEKRVIRGGSWIAPEGTIRSTHRFWNH
                     PLNNSYGVGLGFRCAKTAPPDWEQRIRDSYITALVEMGRERFAEAQQAVARGLAMDPV
                     NVELLELRPLIEQSMKRP"
     gene            complement(29636..30598)
                     /locus_tag="PLS35_03575"
     CDS             complement(29636..30598)
                     /locus_tag="PLS35_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI17765.1"
                     /translation="MRSHIKVQMGIGVLLVAGATSFAIAADASNENKDMVLVPKGEFI
                     MGSNEHSDEVRHQVVLDAYLIDKFEASNARYKEFMRATGHPAPAYWDDPRLSKPEQPV
                     VGVSWTDANAFCKWDGKRLPTEAEWEKAAKGPEGDNHYPWGHHLDPKKANYGQNVGRT
                     MPVDSYPEGVSGYGVYNMAGNVFEWVDDWYDPKYYKTSNALNPKGADKGYNFANQGPV
                     KVLRGGSWLAPETSLHTSHRFWNQPENNSYGVGLGFRCAKSVTAVSEEAAQAGRDAFI
                     QALVGMGAEKNADAMASIEKALAADPNNKEYLATRELIQKSMKK"
     gene            complement(30649..30945)
                     /locus_tag="PLS35_03580"
     CDS             complement(30649..30945)
                     /locus_tag="PLS35_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17766.1"
                     /translation="MATTSALQGQVEQHERRIDRSSFSQEPNCSRCGGLMVRDFCTDL
                     LSGKDGLDCPTRRCVQCGDVVDPVIRWNRHLQQLAGVGREMNTLSQALHRQPSV"
     gene            31083..32000
                     /locus_tag="PLS35_03585"
     CDS             31083..32000
                     /locus_tag="PLS35_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNI17767.1"
                     /translation="MNIHRIHRVAKLTGLSRDVIRVWERRFDLLKPTRGANRYRNYSD
                     DDVALLRYLKQQLDGGASIGDLARLGREELMNRVRAAAPRTIPVDNTFERLLRELLSA
                     LEPLDRVTFEKRLNGAVAVVPFEEALHGILLPLQERVGQLWHTGRINVAVEHYVTSQI
                     KQKLYAAMNQLPVAEFGATVVIACPPQEEHDLAALAVAYRCRVRDCRVYYLGANVPIS
                     SLSKLCREVAPDLTILSITIAPSDPLATDLVQALIHDVAPVSKIIAGGRGALAMRQHF
                     AESGIELLESFGDLDETLERFTRRFPIPG"
     gene            32018..32434
                     /locus_tag="PLS35_03590"
     CDS             32018..32434
                     /locus_tag="PLS35_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNI17768.1"
                     /translation="MVGYPLTVWYDGACPLCSREMAFMKRLDRKRRLQFCDFSAPDYN
                     VAASGIPLSELGAVIHAQWEDGTVIRSVEVFRAIWQAVGLGFLTRMSRLPLIDPLVVQ
                     AYAWFARNRLWLTGRTVCTGGQCRNKSAANAAPPSA"
BASE COUNT         6773 a         9898 c         8644 g         7388 t
ORIGIN      
        1 tctaggacta tcgggactaa agccctaggt cctttgtgcg cttgctatta aaaatgagca
       61 agggctaaga ttccgacctc ttaaatagcc ttataagccc tcaaacccgt tgaggttcga
      121 gctaatggcc ggttccgagc tcctcttaga gtatctcagt cgatatttcc cggtactcgt
      181 ctttgtgttc atcgcactgg cgtttggcgc cggcacgctg attatcagct attttgttca
      241 accgaagtat cctgaaccag agaagctatc cacctatgag tgcggatctg aacccttttc
      301 tgacgccaga atgccgtttc ccgtgcgtta ctatatcttc gcgatgttgt tcgtgatttt
      361 cgacgtggaa gttatctttc tttacccctg ggccattgtg tttaataaga tcggcctcat
      421 tggcttgatc gagatgttga tctttatcgg gcttttcctc gtcgcctatg tctacgcctg
      481 gcgaaaagga gcattggaat gggattaatt caactcggtg gccacgataa agacggctct
      541 cccgatgtca tcaccctcac ggttgaaaaa gccgtgaatt gggccagaaa aggatcctta
      601 tggccgatga ccttcggtct ggcctgctgc gcaatcgaaa tgatcgcggc tgtttcatca
      661 cgctatgaca tggatcgtta tggcgcaggg gtcttccggg cctcgccccg acaatccgac
      721 ctgatgatcg tcgcaggtac cgtctgccgg cgcatggcgc ccgtcattcg caaaatttat
      781 gaccagatgc ccgaaccgaa gtacgtgatc gccatgggct cctgcgcaac atcaggaaat
      841 atttacgaca gttacagtgt ggttcaggga gttgaccgat tcgtgccggt agacatttat
      901 gtgccgggat gccctccaac cccagaagcg ctcttcgatg gcattctgaa gttgcaggag
      961 cggatcatgc agaagcgcgt cttcctctcc cagcccaaag aagtgaagga cgcgctgaag
     1021 gtctgacgga cggaacgact tcatgagcca actagcccaa cgtatcgagg acacctttcc
     1081 tggcgcttgc acaaaagtag tggagtggcg cggggacctc gccgtgacgg tgaagcctga
     1141 gacgttgcac gatgtagcca agtttctgcg ggacgatcct gcattacggt ttgactacat
     1201 cgtccatgtc agctcagtag actggcccga cgatgaagat cggtttgaag tcgtctacga
     1261 ggtctactcg attcgtacac gacaacggat tcgtctgaaa actcgcgtac cggaatccga
     1321 ttgcatcgtc gattccctga ccgacgtctg gaaaggcgcc gattttatgg aacgcgaagt
     1381 ctatgacatg atgggcatcc ggttccgtaa ccatccggat cttagacgca ttctgatgcc
     1441 ggacgaatac gacgagggat atccgttgcg taaagatttt ccgcttcgtg gcaagggttg
     1501 gcgagacacg tttgagtttt tggatgaacc gacccgatag gcaacaggtt tagccccact
     1561 gaacgaccat ggcacagttc gaagatcaaa gaactaccgt ttataaaatt gatcccgagc
     1621 atccggagag cgaaacgctt ccaacgctca ggaccgagga actcctcctg aatatgggcc
     1681 ctcaacaccc cagcacccat ggagtgttga aggtgattct ggagttggaa ggggagcggt
     1741 tggtcaagtc gactccggtt atgggttttc tccaccgtgg cgtggaaaag ctcgccgaag
     1801 aagggacgta tcaccaattc atccctcata ccgatcggct cgactatgtc tgcgcgatgt
     1861 acaacaactt cgcctactgc cgcgcggttg aaaaattgat gaacatcacc gtgccggatc
     1921 gtgctgagta cctgcgtacg atcgtcgcgg agattcaacg tatcatcggt caccagttct
     1981 ggcttggcac gcaagccctt gatatcgggg cgatgaccgt atttttttac tgcttccgtg
     2041 atcgggaaat tctgttggat tggttcgatg agctctgtgg tgcccgcctg acgaccagct
     2101 ggtaccgcat cggcggtgtc gagcgcgact tcaccccgtc tctgtttgcc aaattgaagc
     2161 aattcctcga ttacttccca ccgaagatcg atgaatacgt cattttcctt gagaagaatc
     2221 gtatctggct ggcacgcacg aagggcgtcg ccgtgatttc agctgaagat gcattgagct
     2281 ttggcttgag cggaccaacg ttgagagggt ccggcgttga tttcgacctt cgcaaatacg
     2341 aaccctattc tgcctatccc aagtgtgaat tcagcgtccc tgtcggcaag aacggcgaca
     2401 cgtatgatcg gtactggatc cgcgtgcaag agctctacga gagcgtcaag atcatccgcc
     2461 agtgtctcga acagatccag gatgggccca ttatggcgga tgtacccagc gtgacgctgc
     2521 ctcccaaaga gcgcgtgttc acgaacctgg aatccatgat tcagcaattc aagctgttct
     2581 cacaggggtt caatgcacct cccggagaaa tctactgcgg gaccgaagcg cataagggtg
     2641 agttgggttt ctacatcgtc agcacgggcg gcgggaagcc gtaccgactg aaaatccgcg
     2701 ccccctcgtt tattcatatg ggggcattcg atcatatgtc gaaaggctat atgatctccg
     2761 acgccgtgac gattttcgga acatacgaca tcgtcatggg agagtgtgac agataagcac
     2821 attgagaggt gggtgaaaga agtgtgaact tctcagtccc ctccaaacac ttataactaa
     2881 gtactaggcc aaagcatggg tttgaaacca gcgactaatc cggacgttga agccgcgacc
     2941 attgaactgt cgattgacgg acgaacggta tcggccaagg acggcgtttc gctttacgat
     3001 gtgatcgcca gcaccggcaa gattattccg gccatgtgct accactacac gtttgatccg
     3061 ttcggatcgt gcggcatgtg ccttgtcatg caggaaggga agaaggcgcc ggtacgatcc
     3121 tgcacggcga aagcggcagc cggcatggtg atccggaccg acggtgatga cctcttcgcc
     3181 gcccgaaaga aagcggttga gaaacatctt tcagttcacc cgctggattg tcccgtctgt
     3241 gacgcggacg ggcactgcga actccaggac atggcattcc agcatggcgt cacgaacctg
     3301 gccaacgcca aacaaaagtt cattccagaa gacacccgta gcctggtgct ggacttcaac
     3361 atgaaccgct gcatcgcctg tgcggaatgc atcaacgtct gcaaagacgt gttgatgatc
     3421 gacgcgctgc aattcatgaa gaagggcggt ttcaaccagg ttgtcgccaa aggagaccag
     3481 ccactcgcct gtgaattctg cggggattgc ttggccgtgt gtcccgtcgg cgccatcacg
     3541 aataagtact cgaaatatct ctacaagccg tggcagatga gaaagaccac gacgacgtgt
     3601 aactattgtg gcgatggttg ccaaatgcac ctcgaaacaa aggacacgga agtcgttcgc
     3661 gtgacgtcgc cgctttcctg gaaaaacaaa tggggcgacc ggtctgaaac cgccaagggg
     3721 cacggcggac tgtgcgttcg gggccgattc ggatttcaat acatcgacag tcagcaacga
     3781 ctccggcaac ccctggttcg caccggcgac actctgaaag agacgccatg gctcgatgcg
     3841 atgcagaccg tcatcgatca gttgtcagac atccaccgca agcacggccc ggaatcgatc
     3901 gccggactca ttactgcccg ctgcaccaac gaagaactct acttgtttca gaagctcatg
     3961 cgtggcggcc tcaggaccaa tcaactcgac agtagcgccc gatacggcca cttaaactac
     4021 gtgcacgccg tccgacatgc cgtcgggatt ggccgaccgc tcaatgattg ggaagatctg
     4081 accaaggcca aggccgtcct cgtcatcggt tccaatatta cagaaaccaa ccccctgacc
     4141 gcggtccgca ttaaagaagc catccgtgtg tatcacgccc aagtgatcgt cgtggattca
     4201 tcgaacacga atatcgccca attggcatcg catccactcc tggtgaagcc gggtaccgaa
     4261 gcactgctca ttgatggtct cgtcaaagcc gtcatcgagc aggatctggt cgatgaaacc
     4321 agtacgacac aacacccgca ggcatttgca gccttgaaac aagcagtcgc tcaaatatcc
     4381 ctggacgagg tatcccggac gaccgggatc tcagtggacc agatccgcga ggtggctgcc
     4441 atttttgcgg aagcgccgcg ggccgtggct ttgtgcgcgg aagggattgt ccgccgtccc
     4501 aacggatacg agaatgtcct caagctcgtt gacctggcat gggtgaccgg caaattggga
     4561 caaccaggat gcggtgtcac taccgtcacc gaagaggtga acgaacaggg tgccatcgac
     4621 atgggtgttg ctccagagtt ccttccgggg caggcccgct tcgacgatcc cggggctcga
     4681 gaacgtttcg ggaaggcttg ggaagtgacc cttccggcga tcggcacggg cgccaatctt
     4741 ctggagattt tcacccgcat tcagaaaggc caaatcaagg cactgtatgt cattggggaa
     4801 aacccgttgg caaccttgcc cgcctccatg gatgtgaagg gggccctcga caagttgcaa
     4861 ctcctgattg ttcaggaccc gtttctcacg gaaacaggcc gcatggctca tgtcgtcctc
     4921 cccgcggcta cctatgcgga aaaagacgga acattcacca atctggaagg aaaagttctg
     4981 cgtgtccgcc aggcgcttga ccaggtcgga gaaagcttgc cggattggca tatcatgacg
     5041 gccttagcca atggcctcgg ctacgaatgg ccctatcaat caccgcagga tgtgcagaac
     5101 gaaattatga agctgctccc tggctactac aacttggggc aaccacgccg tattgctccc
     5161 tccgctgacg catacctctc taatggctat tccagcgagg ttgtcacccg gtatcgcaga
     5221 cggcaggcca tggacgacac ctcacggcca tttaccctcg tgatgggaca ggtcctctac
     5281 cattccggga aaatgtccac gcaggcgtca gggctcatta atattgagcc caataatggg
     5341 cgtatacgaa tgaatccggc tgatgtggag aaactcggcc tgaccgagca atccactgtc
     5401 cgcctgagct cgcagcaagc atccgtgcag gccggagtga aagcagaccc ggatgtccaa
     5461 tccggatcgt gctttttccc cgaacatttt aatgagcctc caataaaaga tttgatgacg
     5521 actgaagtcg atgctgtaac cggcgtgccg tatttcaaat caacgcgtgt aaagattgaa
     5581 aaggttggtg cgtaagagta agctcgtccg tctttttgat gtcgtcaaag gtggtgggaa
     5641 tgagtgtcgg ggcgttgacc aaaaaagttc tgcaggcggc gttgttctat gaaatctggg
     5701 acgccatgaa ggtcaccttc aagcacatgt tccatcgccc tattacgttc cagtatccgc
     5761 gtgagcagcg caccattccg gacggccatc ggggtgctct tggcctcctt cgctatagcg
     5821 acggacgtga tcgctgcgtc ggatgcgatc tctgtgaagc ggcctgtccc tctcgctgca
     5881 tcaaggtgat cagccaggaa gatcccgaac gaccgctgca gcgatttgcc agtgagtttt
     5941 atatcgacat caccaagtgc gtcttttgcg gatactgcgt cgaagcctgt ccggtcaacg
     6001 cactggcgat gacgaaaatg tatgaatatt ccacccacga caagcggacc ctcctcttcg
     6061 ataagaaacg gctctacgag gttggtgaac gccacctcga ggacgctaaa aaatatttgt
     6121 atacccataa tcaagaaaag aattcggacg agagccggga gtatcgttac tactttccgc
     6181 agtccgtgct aaagcccaca cagtcgcaac ccaaacattt gagctgaatc gtaccgttat
     6241 ggctttcgtg tttttcgcgt atttcgcagt ggtcagtatt gtcgccggca ttctcaccgt
     6301 ggcgttgaag aatcctgtgc aatgcggttt ggcgcttctg gctctgttgc tccatgtatc
     6361 agggctgttt gtgatgctga acgccgagtt cctgtgggcc gttcaagtca tcgtctacgc
     6421 aggcgccatt ctggttctct acctctttgt gctcatgcta ttgaacctca aaactgacga
     6481 ccgctatttc catgcgagaa ctccgtatct cctggctccg gctgtcatcg ggttgatcta
     6541 cctgctgttc ctactcatcc gttccccgtt tagcggatct cgaggtgatg catctacggc
     6601 agccgttctg ttgaacggag atgcgcatgc ggtaggcatc aagatgttca gcgattattt
     6661 gcttcagttc gaaattatcg gcgtgttcct gacaggcgcg atcgtcggcg cgatcgttct
     6721 ggcgaaggcg cccaaatcta tcgaaacaag gcgggacgca tgattccttt atccgcctac
     6781 gtcgccgtca gtgccattct ctttacgacg ggtttgctcg gggttctcat ccgccgcaac
     6841 tttattatcg tgctgatgtc cgtcgaaatt atgctgaatg cggccacgat caacctggtc
     6901 gccttttctc actacctcga atccatgcaa ggtcagatcg tggcgttgtt tatcatcgcc
     6961 atcgcagcgg gagaggccgc catcggactc gccattatta ttgtcgtatt ccgcggcaag
     7021 atctcgacga acgtcgacga aatgaaccta ttgaagtggt aacgtgtccg actccattga
     7081 cctgatcgta aaactgattc ccctgtttcc gctgatggcc gttattgtga acggtctctt
     7141 cgggcatcgt tattctcacg acctggctca ccgctttgca tggggttcgg tcctcatgtc
     7201 gttcctctgc gtgctggcag tcttcacgga aaccttacgt accggagcgg ctcgcgaagt
     7261 cattgcctat aaatggatct tcggcggcga tctcacgatt aatctcgcct atctgattga
     7321 ccccctaacc tgcatcatgc tgctcgtcat caccggagta ggattcctca tccatgtgta
     7381 ctccgtgggg tacatgcatg gagaagccgg cttcacacgg tttttcgtgt acatgaacct
     7441 cttcatggtg tcgatgctcc tgctggtcat gggcaacaac tatgtggtcc tgttcatcgg
     7501 gtgggaaggc gtcggcctct gttcgtatct gttgatcggc tactactacg acaaggtatc
     7561 ggcggccaaa gcggcgacca aggcgtttgt ggtcaatcgc atcggcgatg caggcttttt
     7621 gctggccatt ttccttgtct tcgtgaactt ccggacactc gattacacgc aggtgatgca
     7681 aaacgcggcg cagctgtcgc ctgaaatggc cacggcgatc gcgctctgcc tcctcgtggg
     7741 agccgtcggc aaatcagccc aattgccact ctacacctgg ctgcccgatg caatggaagg
     7801 tcccacccct gtcagcgcgc tcatccatgc cgccacgatg gtgacggccg gcgtgtacat
     7861 gatcgttcgg aatcacgcca ttttcgacct ggcgccgatg gcgatgacga ccgtcgcctg
     7921 gatcggcgga ttgacggctc ttttcgccgc caccatcggc ctagtgcaga ccgacatcaa
     7981 gcgtgtcctg gcctattcca ctgtgagcca actcggttac atgttccttg gttgcggtct
     8041 cggcgcctac acagccgccg tattccatct tatgacccac gccttcttta aagccctcct
     8101 ctttttgtca gccggttcgg tcatccatgc tctttcaggc gagcaggata tccgcaagat
     8161 gggcgccttg aagtcgaaaa ttccatggac ccatacattg ttcctgatcg gcacgatcgc
     8221 gatcgcggga attccgcctt tggcggggtt ctggagcaaa gacgaaatca tgggccatgc
     8281 cttcgtccat catcactatc tcctgtacgg catggcagca gtcggcgcct tcctcacgtc
     8341 gttttacatg ttccgcctga cgtatctgac attttatgga acgtcgcgac tcgatcatca
     8401 tacggccgaa cacgtgcacg aatcgcccat ggtcatgatc ggccctctcg tcgtgctggc
     8461 cacgttgtcc gtcatcggcg ggtttcctgg cgttccaccg gaaaacggct ggtttcacca
     8521 tttcctccat tccgtggcag gagtcgcggg tgaggagcat gcgacgccgc cagcattgat
     8581 gtttgggctc atggggacgg cgaccgtcat cgcgctgctc ggatggggcc tcgcacacta
     8641 cttctatagc ggcccatcaa ccgccgccgc agcccttgcc gaccgcatgc ccggcgcata
     8701 taccaccttg ctcaacaagt actatgtcga tgagctctac gacaccctgt tcgtggaacc
     8761 caccaaacaa ttgggccgcc tctgcgattg gttcgaccgc accattattg acggacttgt
     8821 ccgcggcatt gaccgaggca ccgacctcag ctctgcggcg attacctgga cggaaaaaca
     8881 cgtggtctat gccggcctca atattatcgg ctatgcgaat cacctcttgg cacgctcctg
     8941 gcgccgcctg caaaccggca tggtacatca gtatgcagct atcatcgtgg cgggactatt
     9001 cattctcgtc cacctcatct tgttgatctg gaccggaggc ggttcgacag gttattcagc
     9061 acgctgacgc ggcagatttt tggatttacg atgcttgaag agttcagttt cgggttcccc
     9121 atcctctcgt acctgatctt cctccccctg gtcggggcgg cggttctctg gctgattgac
     9181 gatgaggatc ttctcaaatc gacgacattg ggtattaccc tcgtcgaact ggcgctcgcc
     9241 tgcctcgtcc tcgtgcgttt tgtccctgat tcggcggcca tgcagttcgc cgaacacgcc
     9301 cggtggatgc cggcgctggg aatcggatat cacctcgccg tcgacggcat cagcgtgctc
     9361 ttcgtggggt tgacggcctt tctgacggtc ttggtagtga tctattcctg ggacacgatc
     9421 cgtaatcagg tgcgcctcta tttcatggcc ctcctggccc ttgaaaccac cacgatcggc
     9481 atttttgtct ctatcgatct aatcctcttc ttcgtgttct gggaactcat gctcattccc
     9541 agctacttcc tgatcaagct gtggggggga ggagccgacc gccattacgc agctttgaaa
     9601 tacgttctct atacacttct gggcagcgtc tttatgttgg tcggcattgc cttgctggac
     9661 attaactatc accagtgggc cgtgagccat cacatggatc acgtgtattc attcgacctg
     9721 cttgagctgc tctccgttcc cattccactc tcgcagcaga ttttgatctt ctggctcatg
     9781 tttatggggt ttgcgttcaa agcgccggtg ttcccatttc acacctggct tcctgatgcc
     9841 ctcgtcgaag gtccgatcgg catggccgtc atgttggccg gcatgaaatt gggcacctat
     9901 ggattcctcc gcttcacctt cccattagtg cccgatgcat caaaaagcga aggagtggtt
     9961 ggcatcgtca tggtattagc cctgtctgcc attctctatg gggcggtcgt ggcgctggtg
    10021 caatcagatt tcaaacgatt actcgcattt agcagtatca gtcacctcgg atttgtggtg
    10081 gtgggcctct ttgcgttgaa tttccaggga ctccagggca gcctgttgac catgatcaat
    10141 ctggggttca gcacggccgg cctctttttc atggccgggt tcctgtcaac ccgccaacag
    10201 agctcgcact tatcgtcctt cggaggtttt gccaagcagg ctccgctcct ggcgaccttc
    10261 cttcttttga tcggcatggc gtcgatcgga ctgcctggca ccaacggatt cgtcggggaa
    10321 ttcttgattc ttttgggtgc cttcaaagcg aaatggtggt tcggggccgt ggccgtactc
    10381 ggcgtgatct ttggagctgc gtattttctg tggtattacg agcgagccat gctcggcccg
    10441 ctcagcaaac acgtgtcggc ggccgtcaag gatctccaca tgcgtgagat caccattgcc
    10501 ctctcgctct ccattatgat tttgtggatc ggcctctatc cctcgccctt cctcagaatg
    10561 atgaatggat ccattcaagc actcgtcgac cgacttgatc gtgcgaaagt ggcctcagta
    10621 gacactgtta tccacgtgcc agcgaagtaa gatatggctg aatacacgct gctcatcatt
    10681 ttgttcgcgc ccttcctggg agccctggga ttaattttcg tctccaaccg tcaagtttcg
    10741 ctggtacgag gcattgccgc cggttcggcg tttgtctcgt tggccgcctc cgtctatctc
    10801 ttttatgcct atgattcagt gaagggcggc tatcagttca tccagcggat tgagtggtcc
    10861 cgacagctcg gcatttccct gcatctcggc gtcgatggca ttggcactcc gctggtgctt
    10921 gcctcaggca ttttgttgtt cgccggcatc ttcgtctcct ggcatatcaa agaccggatg
    10981 aaagagttct acatctggat cctgattctc gctgccgcga ccatcggcgt cttcatgtcg
    11041 ctcgacttat tcttcctgta cttcttctat gaaatgtccg tcatccccat gtacctactt
    11101 ttgggcatgt ggggcagtca taccaagaaa taccttgaaa tgacggaccc cgaaggcctg
    11161 aaacaacgtg actccgtcgg ctttatcttc aacttcggcg caaacagcaa agaatatgcg
    11221 gcgatgaagc tggtcctgtt tctttccgcc tttgccgtgg ccgccttgat ggggattctg
    11281 ctcatctaca aattttccgg actgaacacg ttcgatatcc tgatcttgcg cgagaaagcc
    11341 catttctcag ggccgttggc cacgctcatc tggctcctga ttttcttcgg ctttgcgtcc
    11401 atcgcgccga tctggccatt gcattcctgg tctccggtag gtcacgccgc cgccccggcc
    11461 gctaccagca tgttgcatgc cggcgtgctg atgaaactcg gccacttttc gatcattcgc
    11521 gtggcgttcg aaattcttcc agagaccacc cgagaactga tgcccatcgc cgcggtgctc
    11581 tgtatcttca gcattattta tggcgggctg gtggcgtact atgcgaagga cacgaaatac
    11641 gtcatcggtt attcaagctc aagtcatatg ggctatgtct tcttgggcat ggcggccttg
    11701 gactacatca gcctcagcgg agccgtcatc tacatgttcg cgcatgccat ggccaccggc
    11761 atgctgttcg ccatggccgg ctgggtctac gatcaaacac acactcgaga catcccgtcc
    11821 ctgggagggc tctccaaccg gatgccgttt atctctgcgg ccttcgtcat cggttgcatg
    11881 gcctcgatcg gaatgcccgg gaccgtcaac tttatcgctg aagtgatgat catcgtcggc
    11941 agctggaata aatacccttt ccaagtcgtc gtggcagtgc tcggcatcgt gttgaccatg
    12001 gcctacctct tcaagatgat gcgcggcctc ttctacggtt cgatggccga aaagtatagc
    12061 cactcgcacg acgccgtggc agtggtcgat cgcatgcccc tgctcattat gattacggtg
    12121 agcatcagct tcggcatttt cccgggacat ctctactccg tggtccgatc aggggtcgac
    12181 ccgctgatcg cgcgtattac caaagtggtc ccggtcgcag agcttccgat gaacattcca
    12241 caagccagct cgcacaccat gcccgtgcct gttcccggtg aggcgattgc gaaggtaacc
    12301 tcgcgatgac tttctccctg acgctttcag ccagcgatct tctcctcctg cttcccgaac
    12361 tgtttctcac gctctggctg tgcgtcgtcc tggccgtgga tttttcgttc aaacgcatcg
    12421 tccaggaaca attggcctac ctgaccatct tgggagtgat tatcaccctg gcctgcctcg
    12481 catggttcga catgaccggc atcaccggca ccctgttcgg gaagatgttc gtcgtcgatc
    12541 gcatggcgat cttcttcaaa gtcatgatcc tcctggccac gatcctggtc attctgcttt
    12601 cgatcgacta tgtgcatcgc ttctcgctct ttcgaggcga atattatttc ctcgtcgcga
    12661 tgtcggcgtt aggcatgatg ttcatggcct cggccaatga tctcttatcg ctcttcgtca
    12721 ccctcgagtt tgcgaccttc ggcttttacg tgctggtctc ctatctgcga gacgacatgg
    12781 cgtcgaacga ggccggcctc aaattcttta tcctcggagt ctttgcggca ggattgctgg
    12841 cgtacgggat cagcctcgtc tacggcgaaa ccggcaagct cgtcttctcc gacatgagtg
    12901 gcgcgaaccc gacgacggga ttagtcatcg gcttcctgct gatctttgcg gcattaggat
    12961 tcaaaatcgg cgccgttccc ttccattcct ggatccccga tacgtatcac ggcgcaccga
    13021 cccctgtgac ggcgttcctg tccatcgccc cgaaagtcgc ggccttcgcc atcctgcttc
    13081 ggttgttcct tgtcgcgctg gccacgttta agcccgcgtg ggccttgctc atcgtcgccg
    13141 catctattct atcgatgacg tacggcaata tcgtggcaat cgcccaacga aacattaagc
    13201 gcctgctggc ctattccggt atcgcgcagg tcggaaatgt cctgatcggt ctggccgcag
    13261 gaaccaagat gggcactgac tcaatcttgt tctacctgtt gacctatctc ttcgcaaacc
    13321 tcggcgcctt tgcggtcatc atggcgatca gcaactccgt gggcagtgat gagatcgacg
    13381 attatcgcgg actcaaccgt cgctcgccct ttctggcgtt tgccatgtta attttcctgc
    13441 tctcactcgc aggggttccg ccactcgccg gctttatcgg gaaactctat atctttgtcg
    13501 ccgccatcaa agaaggcctc tacattctca ttaccgtcgg cctcgtcaac atcgttgtct
    13561 cgatgtacta ctatctgatc gtcgtcaaga agatgtacat caacgaaccg ctcgactcct
    13621 ctcccatcaa gaccacgggt ccgctgcgag cagtgatcta tgtcggcctg gccgggacgc
    13681 tcgtaatcgg catctacccc caaccgttcc tcgattgggc tgtagcagct acgctgatgt
    13741 tttccaatct cattggaccc gctgcttccc tgactccgcc ggtcaccccg ttcgggggct
    13801 gaggattcgt ccggcttcgc ttttctcggc ttccatcaat tctcttcgca tatcagtacc
    13861 ttctggtaca atgtgctgtg cagtcttccg actgcgtcaa catcattgac gcgcaattcc
    13921 caggctccag tccccagatc tcggagcttt cactgcaggc atgaacgaac caaaccgcca
    13981 tcaacccgtt gacgtcgggc tcgactccgc atttcctcct gcctcgccgg cggaagaaaa
    14041 gagttccggc cgagtatcgt tcctctcgcc actgcttcac acgtttcaga agcgatctat
    14101 cgaacagcgc gtcctggctg gattcgggtt ggtttttgcc ggcatcctgg tcatttccgc
    14161 catttcctat cgcaacatga cggtcctcat tcggaatggc gatttagaca agcgaagtca
    14221 cgagttcatc cagtttctcg gagcaaccgg agaagcgatt gacgacgctg agaacgggca
    14281 ccgtcgattt ctcgtcacgg gagacgagag ttatctggcc gcctacaaca ccttgcggga
    14341 ccgcgcgccc gaatatattc gctatttgcg ggagctcacc gatccaggca gcacacagcg
    14401 tgggcatgtc agtgatctcg aacatttgat caatcaacag ctccaagaag aacatgccgc
    14461 catcgaactc cgcgccaaga cagggtttga ctcggtgcgg ggaatggcgc tcacgggggt
    14521 cgccaagacc gcactcgatt cagcgagacg actgcaagcg cagatggatc aggaggagac
    14581 gaaagccttg acccaacggg tcgttgaatc aacaaccacg actcgctcca gcatcattct
    14641 gttgaccatt ggggcgctct tgctgttcgt gctcctcgcg gccgtgtatt acctgatccg
    14701 gcacgatatt accgaacggc gccggattgc cgacgaactc caccgccggg gagaacttct
    14761 tgaagccgcc aataaggaac tggaggcgtt cagctattcg gtgtcgcacg acttgcgcgc
    14821 gccactccgg cacattgacg gctatgcctc gctgctcgcg aaagccgcgt ctatctcact
    14881 cgacgataag gcgaaacgct acttacagac gatttcggaa tccgccaccc gaatgggaca
    14941 actcattgac gatctcctgg tgttttcgcg catggggcgg caggaaatgt tgcgggggac
    15001 agtcaatttg aaccaattaa tcgccgcatt gcggcacgat cttcgacatg acttgcaaga
    15061 tcgcgcaatc tcatggacaa tcgctcaact ccctgaggtc acgggagatg ccgccatgtt
    15121 gcggcaggtt ttcatgaatc tgatgacgaa cgcgattaaa tttaccggga ccaggccaca
    15181 ggccacgatc gaaatcggca gtctggacaa cggcccggat gaagcggtgt tattcgtacg
    15241 tgataatggg gtaggattcg atatgcgtta cgccaacaag ctctttgggg tgtttcaacg
    15301 gctccatcga gcggatgagt tcgaagggac aggcattgga ttggccaatg tcaggcgaat
    15361 tattcatcga catgggggga aaacctgggc ggaaggtgcg ctcggcgaag gtgccacctt
    15421 ttacgtcagc ctcccgagag tgaggccctt ggcatgatcg cttctaaacc cattttgctc
    15481 gctgaagaca atccgcgcga tgccgaactg gcgctggctg cgatggaaga gcatcatttg
    15541 gccgataagg tcatcctgtg ccatgatggc gccgaagtgc tggactacct ctattgccgc
    15601 ggccatttca aaacgcgtct ccaaggcaat ccggccgtcg tgcttctcga tttgaaaatg
    15661 cccaaggtcg atggccttga ggtcctgcgc acgatcaaga acgatgcggc cctcaggccg
    15721 atccccgtcg tgatgctcac ctcctcacgc gaagaacgcg atttggctga gagttatgca
    15781 ctcggagcca acgcctatgt cgtcaagccg gttgagtttc atcagtttct gaaagccgtc
    15841 aaagaactgg gcgtgttctg gggcatgatc aacgaaccac cgcccgaagg agccagccgg
    15901 tccgccggcc ggtccagcta attccgatct cgttgcagat gatgactcag cccctgcgat
    15961 tcatccatct tgagagcaac ccggcggacg cggatctgat cgactccacg ctccgggagg
    16021 cgggtattct gtgccaaccg aagcacgttc acacgcgtga agactttctc gcagccctcc
    16081 gccaggaaag cttcagcctc attcttgccg acaccaccgt tcccggtttc gacggcgccg
    16141 cggcgctcgg tctcgcacga accctgcatc cggatgtgcc gttcctcttc gtctccgcaa
    16201 cgcaggggga agaattcgcc atcgatatga tgcagcgcgg ggcgacggac tatatcttta
    16261 aacaacgcct gggcagactt gtcccatcca tcaaacggac cctgcgtgaa cttgacgagc
    16321 gtcttgaacg gagacgggcc gaagacgcgt tgcgcatgag cgaaaaacag tttcggcagg
    16381 cgcagaaaat ggaggccgtc ggccgccttg caggaggact ggctcacgac ttcaacaacc
    16441 tcctgacggt catcatggga cacagccaag tgctcctcgg ggaactgccc gccggcaacc
    16501 caattcgtgt aaagatcgaa gagatgcaga aagccggaga gcgagccgcg agtttaattc
    16561 gccaactcat ggccttcagc cgcaagcagc cagtagaacc gcaggtgctc ccattaaatg
    16621 ccatcctggc caatgtagag ggcatgctcc gtcgtctcat tggcgaagat attcaattgg
    16681 tcatccgtcc cgacccgcat cacggccatg tcaaggccga tccggggcaa ctggaacaag
    16741 tgctcatgaa tttggtcgtg aatgcccggg atgcaatgcc caacgggggc ctgctggcga
    16801 ttgaaacatc gcaaacggaa ctggcgcgca cgcccatgca tcatctccat cccttgccgt
    16861 tgggacacta cgtcaaactc acggtcaccg acaccggctg cggcatggat gccgaggtcc
    16921 tcgcccactt attcgaaccg ttttttacga cgaaagaaga acacaagggc acgggcctcg
    16981 ggctgtccac cgtcttcggg atcgtcacca cctgcggggg cggcatcgat gtctggagtc
    17041 aggtcggaca cggcaccacc ttcgacttgt atttcccgcg cgcaaatccg cagacctctg
    17101 ccgctcgttc ggagaaaccc caaagccggc cacgcgaagg atcggagacg atcctgctag
    17161 tcgaagatga cggcggcgtg cgcgacttga ttcgtcatga attggtcaaa accgggtatc
    17221 aagtgattga agcgaagaat ggcgtcgaag cctgcctgac cgccacgcag caaagctatc
    17281 atgtcgatct cttgctaacc gatgtggtca tgccgggcat gaacggccgc gagctcgcgc
    17341 agcacctctc cgtcatcaag ccgaacctgc gagtgctgtt catgtccggg tatctcgacg
    17401 atatctgcgt caacagcggt atggatcccc atcgaaccac gtttttacaa aaaccgttca
    17461 ccccggatat tctgttacgc acggtgcgtg ccttactcga ctcgtcctct cccgcaacgg
    17521 gccagactca acacccgttc tctccaccgg accaagcgcc gcgcaccgct cgcgcctcct
    17581 aaagaaacgt gctcatctga ccgatattcc cgacggaatt gacccctgcc cccatccttt
    17641 ttatactgag cccgcgcgag acgtttcacg ctgccatggt tggctgatta ccatgaaaga
    17701 ccacctgctc cgatgatcaa tccgcaccga tacttaccag gatcccttcg caggatgatt
    17761 cccctctgcg tctgccttct gtctctgtgt catggatggg ttcagacggc agccgcttcg
    17821 gcagaaccag tcccctggga gcgattaacc gcaggcatgc acgtggcgtt gtggaacccc
    17881 atggaggcct gccctcaggt tccctcgctg ctcatgctgc agatcgatcc ggagcggtac
    17941 cgtttttcca tccatcaatt tcgcgatgaa gggttgcggt cgccaatctc gattcacgac
    18001 tggcagcaac ggaccgatgc atatgtcttg ttcaatgcgg gcctgtttcg ggaggactat
    18061 tcgtatctgg gcgtgctatt gaaggagggg cgttcgctcg gcaccaagaa acaccattcc
    18121 tggcaaggcc tgttcgccgc cgaacccacc gacggaacgc tgaaaaaagc acgcgtgctg
    18181 gatctcgcct atgagggatt caccgaagat ccaccactgt atcgagaggc cgcacagtcg
    18241 ctgatgctat tcgaccggac tggcaagctt cgggtcaagg atagcggaaa acgggcgttt
    18301 cagaccgtgg tggcggaaga cgggcaggga gccattcttg tgatcaagac agtggacatc
    18361 gtctcgctcc accacctggc cgactgttta catcgccaac tgccggctct tcagcaggcc
    18421 atggccatgg atggcggcgc atcgtccgat gtcattgcca gccccgacct gttgcacgcg
    18481 gcccaggaaa cctcctcgca ggccggatgg cgatcgcttc ttgctgggac gacaggcgta
    18541 cacattccgc tgcccacggt gatcggcatc acaccacgca ccccttcgcg cagcacaccg
    18601 gctcacgggc cgtcacagtc cactcgcgca cactgatcta tcggccaggg gagactggaa
    18661 atcctgcctc ggtccaggca ttcatgcttc cgaccacatt gtacacatgc gtatagccca
    18721 gatcggccag agtctccgcg gcaatattgc tgcgatgacc agattgacag tagacgacga
    18781 tatggtcttc caacttcgcc ccgagctcac gatgtcgcgc tttcatctcg cgaaaatcaa
    18841 tgttcagatc ggtgccggga atcatgccgg acgcatgctc ttcaggggaa cgcacatcga
    18901 ccagaacaaa ccccttggca ttcgtagacg aggctttcat gagaccggcc cgcaattgct
    18961 gcacgctcaa cacataagag tgataggcga cggccggctg gggtacatcg agcagaaaaa
    19021 gaagcccaca cagacagagc ccacacatga tagacgtcag gtatcgcatg gttatctctc
    19081 tccttttggc gagccggaat aagtgtgcga catatgctgt atgataaaag ccctatgaaa
    19141 aggtggtcaa gctgcacagt ccttactgtc tcgctgtttc tatcaagctg cggcgagggc
    19201 gcccttctgg tgaaggacag tgccgacgga ggcgtggtcg tgtaccccta cagagcagaa
    19261 caggggaccc tcctgtcgtc gttccgaaaa gaggccgtgc ggctgatgga gcagaaatgc
    19321 ccgggcgggt atgcgatcgt tcgagaaggg gaaacgaagg gtcgcctgcg tcaggctagc
    19381 cctgttgcgg gatcaagcga cgtggtcgag gaacggcggt ggggcattca gttccaatgc
    19441 aaatagtcag ataggaatcg ggccaccctt catcagatcg tcaatcgtca acagactcat
    19501 gactttcact ccagtctgct cgacccgggc gcggccatcc tgttcttttc gatcgacgat
    19561 caccagcgcg tctgtgattt ccaatccagc ctcacgagcc gcgacaatcg ctttcaccac
    19621 agatccgccg gacgtcaaca cgtcatccac gatcaaggcg cggtcccccg ttcgcacgac
    19681 accttcgact aatcgaccaa gcccatggtc cttcgcctgc ttccgcacaa aaaaggtgcg
    19741 ccactcgcgt cgaggagcag ccgaataggc ataatccgag atggacgtgg cgatcgagat
    19801 cgcgccgatt tccagtccgc caaggcaatc gatctgtagg tccttgatga cctcccaggc
    19861 tttcttggcg actaagtggc gggcgtgggg gaacgccatt aacgtccggc aatcgacata
    19921 aaacggactg agcaatcccg aggcgagctt aaagccgcct ttcggatccc acttgaacga
    19981 ctgcgtcttg tgaaacgcag cgataagttc gtcttgcagc atgataggtc ctgcgctccc
    20041 ttgaaggccg attcatcaag ccaccgtagc agtaccggcg cctcgcaacg gcggctgatt
    20101 gtacaccggt ctccactcag atggcatcaa atgattcacc gacctgacag ccgcagtcct
    20161 gtccatcagc tgcgttcaca agacccctca accgattgcg attctcctca agagaatcga
    20221 cggacctgtt cagccagcgt cccggcgatc atgccaaggt caaacggcca ggcataccgg
    20281 agctcgacgc cagggtactg aggccggagg tgttcgagaa tttccgggat ctctacttcc
    20341 gagtgagatc cgcctggggt aaacatcgtg gtcgccacgg taatgtgggt ggcaccctgc
    20401 tttgcgagtt gttcgactga ttcttccaac gtcggcgcac aaaactcgtt ataggccacc
    20461 gcaaacaata catcccccag cttcgcgcgc aattgcgccg ccaccgcctc gagtcctgcc
    20521 tgataaggat cggtctccgc attcctcggc cattgacgga tcttggcgtc cagctccatt
    20581 tcttcagctg aagccggttg cttcgcggca cgccgctgcg cctccagtcg tttgagtttc
    20641 gtcaccatgt cttgaggaca ccctctggga atcccgccat gtcccaccag aatcacacct
    20701 ttccgcactg ttgccatttc gtcagtcctc cattcaatgt cacatccacc gatacttact
    20761 tcgcaacact ggcctctaag aattcctgca aattggtctt gccgtaccgg ctcgccggac
    20821 acaccgtgcg aataaatttc gccacgagcg ccatcaccct tttcacagcg gcagtttttt
    20881 cggggtccgt ggcaggcagc agccgaacgg tgatggtggt gtcatgttgt ttcaactgaa
    20941 taaaaaagct ccgtgccacg ccctgttcga tcaccaccgc atcgagcagc gcctccctgc
    21001 cgctcttcgc caggtagaat tcctggacct tgagaatttc ctggtcagcc cgttggagca
    21061 tcggggcgaa gttctgatag agactctgaa ggtcaccggc accctcgacc acgacatgcg
    21121 gcatgacgtc ctttctagac gtgattccgg agcatcagct ccttcggatg cggattcaga
    21181 tacacctgct cgcgaatata ctcgacatcg aagagctgca cataatgttt gatcagcgtg
    21241 agggggacga tcaacggcgt gagtccttga tggtaatccc cgatcactcc cagtaactcc
    21301 gccttttctt gcgcatcaag ccgttccttg aaatgtccga ggatatgatg cagcacattc
    21361 acatgtttgc gcaccgtcgc tcgaaccgcc agggcctgca tgaacaattc gccatattga
    21421 agcgccagat ccttagggcg atactcgctg acccttgcca ccaaacgacc cagctggcga
    21481 tagtgaggtg cactatgcgc cagcagtagg tacttatgga cggtgtgaaa ccgcaccact
    21541 gcctgcctcg tcgcaccgct gttcatcagg tcccgccaac gccgatagca gaatacccgt
    21601 tcgataaaat tctcacgcag caacggatca cagaggcgtc cttcttcttc tacgggtatc
    21661 aacggaaagc ggccaagaaa cgcccgcgcg aatagcccca cgcccttgcg acctggcatg
    21721 ccgtgctcgt tgtaggtccg aactcgttcc actccacaac tgggcgaatc ttttttgaac
    21781 acaaagccgg ataagtccgc gccggccaac tcatcgaggc gcctgtccgc catgacttcc
    21841 aaggcgcggg tatgatcctg gccgctcttg agcgtgacca accgcggctg ttgcggattg
    21901 ccgaccaacc gcatagcctc tctcggtgtg cccagtcccg cctcaacctc aggacagacc
    21961 ggaacccatt ccacataacg gcctaatacg tcggtcaaga aggcatcccg cttgtggccg
    22021 ccgtcgaagc ggacctcctc accaagcaag caccggctga tgccgagacg gagcgtcgcg
    22081 gccgtcacgt cgagaccttc tgtttgccga gatccagata ttcctgccgg gcctgccgat
    22141 gattcacgat aggctccgga taatccgatc cgatacggca tccgacgcgg atttgttctt
    22201 cttgcggcat gagctgcggt tcatgaatcc attttgtcgg cacggaagtc aattccggca
    22261 cgtagcatcg cagataatgc ccatcaggat cgaatttttc actttggagc gttgggttaa
    22321 agatgcgata cccctgcatc gcatccgttc cggtagaggc acaccactgc cagtttccgt
    22381 tgttggccgc cacatctcca tccaccaggt gctgcatgaa gtatcgctcg ccgctttgcc
    22441 aatcgatccg aaggtccttg accagaaacg acgccaccac catacgcaca cgattatgca
    22501 tccagcctgt ctgattcaat tggcgcatgc cggcatccac aatcggatat ccggtacggc
    22561 cttcacacca ggccgcaaag agccggtcgc gctcaggacc ggcaggacga ggagcgggaa
    22621 gtcccggctt gaccttgaac ggcccttcgg tgacatgggg aaaggccatc agcacttgct
    22681 gaaaaaattc tcgccagacc aattcgtcca tccaggtgaa cacgtcgggg cgggatacct
    22741 gaccgccttt ggagagggtc tgtaacgcgg catgcaccat tgtccgcgtc gagatcgttc
    22801 cgaatcgcaa atgaggagaa agcttcgacg tcccgtcttg cgctggaaga tttcgtccac
    22861 tgacataact atggaccggc tttttcaaaa accactgcag tctggctcgc gccgcctgtt
    22921 cgcctggttc gatccacatc ggtaccgatt cgtaacccaa ctcctgcacc gtgggccacg
    22981 ccgacacctt catcgctgta gacgccgcgg ggaatgacgg cacggatagg agcggtggcg
    23041 cagcagaccg ccactgagtc caccagcgat cacgataggc gctataccgt tgaaaggggt
    23101 caccggttaa cccccgcact tcttcggctt cgaacaccac atggtctttg aaggtcctca
    23161 ccagacgccc ctgctgagcc aggcgctgtt gcaccgtacg atcgcgcgcg accgccgccg
    23221 gttcgtaatc acgattccaa tagacggcat ccacggcaca ttcctgcacc gtgcgtagca
    23281 ccgtgtcaat cggctcaccc attcgccaga caagtgtcac accctgttcc gccaaggagc
    23341 ggcgcaaatc ctcaagacag cccaacatga accccacgca gacggatccg aacacatggc
    23401 tggccagcaa tgggtcgtcg aacacaaaaa gtggcacgat ctctttgcat tccttgcaag
    23461 cagccgaaag agcagggttg tcgtgcagcc tcaagtcacg acgaaaccag actaaccccc
    23521 tcataaatga acctcgcgag acgaggacgc aagccgcgtg tcgggcgaca gcggatcaac
    23581 aaacagcact cgtcgccagg ccacatagca gagaaacagg gccgtcgtgg ccatgaaccc
    23641 cgtgcgcagt tgatggaaac cgaattgcca ttgattcatc agttcgctgg tgaggccgaa
    23701 ccccccgtag gccatggcca atccccacgc ccagggacgc agaaagataa atccgtagcc
    23761 gatcaaaaaa tgggcggccg gggaatgcag cttcacaaca taggccatcg ggccggtaat
    23821 cgtcacgccg aatagtttga gcgcataggc ggggaaccat tcaatgatga gtacatccag
    23881 caggcccacc agcaaaaaga gcgccccgaa taatttgata tcgatgccgc gttgactcgt
    23941 ctgctggatc atggttccaa cctccttccc tggacacgtc ataccgtcgg gctccatcgc
    24001 gtcactgcac ccaggcatcg gggaacgcct gccaccaggt cgtgggatgc gcctgtcgcc
    24061 actgagtctg ctcctgccag aggcgatcgg aggccacttt gttcaaccgc accgccatcg
    24121 cggcgctgac gtcagactgt tggcgaacgc tggtttggat catttccttc gccatgcgtg
    24181 cgagaccacc gggcggatcg acgagatcct cggtatgccg gtacgccagg ttcagataga
    24241 ggtgctccac tgccatccag ctttcatcct tggacagctg ttcaagatac gcgtcaatgg
    24301 cctggtacac ataggtcaac cgaacatagg cctcgactga cgggcgctcc tgtaacgcct
    24361 gttcacaact ctccagcgcg cgacgatagt caccggccag cagatacagc cctgtttttt
    24421 cgacccccga tagcgtcgat gccggcgtgg gattcgccct cacctcggta aggcatactc
    24481 ccgagatcat gacgaagcat ccgagcaggg cgatcatacg tttcacacat cacctatttg
    24541 gcagagccgc catgtgatcc catctggccc ttgtacagtg tctgaccggc cacccgcgtc
    24601 acggccactt cgcgcacggc agtttctgct ttgattccca acctgacaat cgtccccggt
    24661 tcgcctctga ccatcaaggc cacctgctcg tacgtcttgc cggtgacgac cgcaccatcc
    24721 accgtagtaa tttcatcccc cgacttgagt ccggcttttt ccgccggccc atccggcaac
    24781 acctgtgcga tgtacaacac tgccgtgtca ccgactcgct ccgctccgac gtgcaacgag
    24841 acgccgatga cgccgtccgg aatcgtgacc tccaatccat acgaccctcc gggagtcccg
    24901 gacttctcat gtccctccgc ggcccaagac ttgccggttc cctcgggcca ctgactcaaa
    24961 cagaggctca agaccccgag ggtcatccat gccaccctat acgtgtgttt gcgcatcatg
    25021 cactcccttc ttcagaccga ccgttaagag gccaacccta ccaagcacag ccctcccagg
    25081 aggacgagcg aattcaaata gagcgaccgc tgatgcaatc gctcccaaga ggccttcaga
    25141 accggatcca cggaaccacg actttcctgt tccttaagct gatctctcat cgcattgctc
    25201 tgtggggtca gtggcgaccg gcaataggct tccgccagga agatgatcag ccagatacca
    25261 cccgctgccg acaggcccgg gctggcccca cgaatgacca agagtgcggt caccgacacc
    25321 aaacccccgg cagccgcgcc cattccgagg agatagtacg cagggaacaa ttgccgcacg
    25381 actttcccga actgctccgg ggccaactgc tttgccagaa cgggagccac cacaaacgat
    25441 aacagcacca ccttgcccac cagtatcgcc gcagccaggc cgtgcagata ctgcatgatc
    25501 gattcaacga ggaggctcat gcccgctgtt cctttgtcat aatcgcgcgc aaagccggtt
    25561 ccagtgtcgg atatcggaac ctgtagcccc ctgcttgtgc cttagctggg tcgacgcgtt
    25621 gtccggttgt catcaacgtt ccgagctctc ccaaacccag acgcaatgcc agcccaggaa
    25681 ccggcaacca cgatggccgg tgcaagacct tgcccaggat gccgcagaag gccttcatcg
    25741 tctccggccc aggcgcaacc gcattcacgg ggccggaaac ctccggggag tgtagagccc
    25801 attgcatcaa gccgatgaga tccgcccggt gaatccagga cacccactgc gtccccggca
    25861 agaccggacc acccgcaaac aaacggaacg gcagcatcat tttcggcaat gccccgccat
    25921 cccgttccag aaccatcccc gtccgtagaa gcacgacacg ggtgccgaac tggccggccc
    25981 ggagggcctc cgcttcccag gctgcggata attgagcaag aaatccttca ccgagggggg
    26041 atcgctcatc caggacacga tcatcctgga ggccgtagta gccgatgccc gaggcgctta
    26101 tcagcacagg gagcggtcga gacctggttg ctatcgcatc caccagcgat ctggtggcga
    26161 gaatacggct ctcgaaaaga atccgcttgc gggcgcttgt ccaccggcct tccgcaatcg
    26221 gctcgcccgc taaattgacc accgcatccg ccgccgcgac cgcgtcctgc caggggccga
    26281 ctgaccgcgc atcccactcc acatagcgta ccttcgactg agtgggggaa tgtggccttc
    26341 ggctcagcac cacgacgtcg tgacccgcat gcgacaattc ttccacgagc gcatggccga
    26401 tgaagcctgt ccctccggtg acgacaatat tcatgtggga gcctccagtt ccggcagtcg
    26461 tccgctacgg ggcgcgatcg gcgccagtct ccgagtcctt tcctttatcc ggtgccgcct
    26521 cgcgatggga cgacttcttc gacgcaccgc cattggaaac agccgtcaca cgctgactga
    26581 actttcgctc tttcgtacca tacagcaaat tgggtggcgg actatgccga ttcttgagga
    26641 tcacgggatc cagcacttcg ccgcacatgg cgcaatgcca cgccgtaata tcgatttggc
    26701 ctgtatcatc caggagatca atataatgct gcgtcaacaa taaccctccg caacggggac
    26761 agacgtgctt gcccggctgg ggaggcataa atgattctgg tgtcccatgt gtgtccattg
    26821 gaacccccga cttcgataaa aaataatcgt ccgcaccgta cctttcgctg gtgtgttggc
    26881 attccgctcc gacatacatg gccgctcgtg gcactagcgg acacgaggat gctcccggct
    26941 cgatcgcctc accatgacgt aggccgggcg ccgcaggcgc acatacagca ccgcaccgat
    27001 actgaccacg aaggccgcca tgcccaagat catttcctgc gtcatcccta cctccttcat
    27061 cccgctgagc gaaacgcccg ttcttgcacc ctctggtggt tcgctgaggg gacaggtgcc
    27121 tccaaaggaa gcacccatcc gcctcaacgg gaggccgcgt gcggagtcgc ggtacctcca
    27181 tgctcaccac agttctcgtt tcaacgaatg cccccatgaa cccatcgctt acctggcttt
    27241 cggctcagcg ccggatgcgt cctggtgagc ctgaccgtca aggctccgca cacagcgaaa
    27301 ccctgtcccg tgtgtttgaa gcgcgaatcc tccccggcct cgtgctgtcg aggtgatgtc
    27361 tgaatgcggg ctatgccagg atcctcctcg aatcacacgc acgataccct tgtctggtcc
    27421 ctggggattt tttgtcggag cattcgcgta gtaatccggg tcataccagt cttgaaccca
    27481 ctcccgggca ttgcccgaca tatccttgat cccgtacgga gaatcgccag ccggcaaggc
    27541 ccccacaggg tgaagcgtct tgtcctccac ccactcgcgg tcgtagttag ctcgcttcga
    27601 cgtcggcgtg tcctcccccc acggaaaggt ccggccatcg gttccacgtg cggccttttc
    27661 ccactccgct tccgtgggca atcgtttctt ggcccaggcg caataggcct tcgcgtcata
    27721 ccatgtgacc tgcacgaccg gcagttgttc gattcccttg gccgacacca gcacaccggt
    27781 tccgtaggga ttgggcggac tccggtggcc tgtggtcgtg acgaatgtca tgtaccgttc
    27841 attcgtgact tccacttcgt ccatggcgaa ggcatccacc tgcacagtgc gttgcggcca
    27901 ttcgtcggcc ctccctttcg ggctctgcgt gcccatcaag aacggtccgg ccggaatggt
    27961 taccatgaga accggatcca gctctttgac agccggcgtc gcaaacacag gggacagtgc
    28021 gagcgtagag gcggccgcca acaggcccgc atatccccat ctggtacgtc tcctggtcaa
    28081 accttgtgtc atttcttctg ctccttgctc ccctctattg cccgttccgt gtgagtcttg
    28141 atctgcgcga cgagcttttc caccgcacct gcgtcgccac ggtcggccgc ttcctgcgct
    28201 ttggtcacga gggttctcgc ttcatgcaaa tgttgttcga tttgtgcttg cagatcgggt
    28261 gacgccacgg ttccccccag cgccgcacga tggtacacct cccaggcgtg atccacgctg
    28321 tgctccgcct catgcaccag ttgcaccttc tccggatggg tgtggcggct ggcgtcaatc
    28381 ggccctgcct gaagttccat cgcggatagc agcgccgcca acccaccgat gattgtgacg
    28441 attgtcctgt gcctcatacc tctgcctccg tcataagaaa ctcttgggtg cgacagtcct
    28501 ccgtccgacg ttacggacgt ttcatcgatt gttcgatgag gggtcgcaac tccaacagtt
    28561 cgacattcac gggatccatg gctagtccac gagccactgc ttgttgcgcc tcggcgaaac
    28621 gttcccggcc catctccacc aatgccgtga tgtaactatc ccggatacgc tgctcccagt
    28681 ctggtggcgc cgtcttggca catcggaagc cgagccctac gccgtagctg ttgttgagag
    28741 gatgattcca gaagcgatgc gtcgagcgga tcgtcccttc cggtgcaatc caggaaccgc
    28801 cgcggatcac acgtttctcg ccaaccgtca gccgatccac ataggtcccg gtaccaccaa
    28861 gccacaaggg tttttccggg ccagtggggt tcaccatcgt ttccagtcgt ccgtagtatt
    28921 tcgggtcgta ccagtcgccc acccactcaa agacattgcc cgccatgttg tacacgccgt
    28981 aatagctggc gccctcagga tgggcatcta ccggctgggt cgcgttatgg tgccgatcat
    29041 agttcgcttt attcgagtcg aagtcgttgc cccatgggta gagattgccg ttaggaccac
    29101 gagccgcctt ttcccactct gcctccgtcg ggagccgctt accgcgatac tcgcaatagg
    29161 ttttcgcgtc gatccagttg accccgacca caggctgttg aggcttgttc aatcgcggat
    29221 cgtcccaata ggccggagcg gggtggccgg tcgccttgat gaattccccg tagtctttgt
    29281 ttgacacctc gtacttatcg atcagatagg agtccaggaa taccgtgtgc gcaggtcctt
    29341 cgtcgtggaa ggcctccgct gaccacggca tgctcattcg cttgtcgtac agggaaagac
    29401 gacgggtcgg tgcggccgct tccgtcctat ccagtcccat gatggacggg ccttgtccca
    29461 cgtacaccat atccttcgga atctcggcct cgtgcgcctg agcgacagaa gcccctgcga
    29521 tgatccccat gacgagtccc gccctgacta ccgttcccat gacagtcatg ccgcacctcc
    29581 tcctgctgaa ggtccgacag ggaggtggtc atcccacctc ctctgcggcc agcatttact
    29641 tcttcatcga cttctgaata agctcacgtg ttgccaggta ttctttattg ttcggatccg
    29701 cagccagggc cttctcgatg gaggccatcg catctgcgtt cttctccgca cccatgccca
    29761 cgagtgcctg aatgaaggca tcgcgtccgg cctgggccgc ttcctccgac accgcggtca
    29821 ccgacttcgc gcagcggaag ccaagtccaa cgccgtagga gttattctcc ggctgattcc
    29881 agaaccggtg gctggtgtgc aatgaggttt ccggagcgag ccatgacccg ccacgcaaaa
    29941 ctttcaccgg gccttgattg gcaaagttgt agcccttgtc cgcgcccttc ggattcaacg
    30001 cgttgctggt cttgtagtat ttggggtcat accagtcgtc gacccactca aagacgttac
    30061 cggccatgtt gtagacgcca tacccgctca cgccctcagg ataggagtcg accggcatcg
    30121 tgcggcccac gttctgtcca tagttggctt tcttcggatc gaggtgatgc ccccacggat
    30181 aatggttatc gccttccggc cccttcgcgg ccttttccca ttccgcttcg gtaggcaagc
    30241 gcttgccgtc ccacttgcag aacgcattcg catccgtcca gctcactccg accaccggct
    30301 gttccggctt gctcagacgg ggatcatccc aataggccgg ggcggggtgg cccgtggctc
    30361 gcatgaattc cttgtagcgt gcattcgaag cttcaaactt gtcgatcagg taggcatcga
    30421 gcaccacttg gtgccgcacc tcatcggaat gttcattgct gcccatgatg aattcaccct
    30481 tcgggacaag caccatgtcc ttgttctcgt tgctggcatc tgcggcaatg gcgaaggatg
    30541 tcgcccccgc caccagcagt actcctattc ccatctgcac cttgatatga ctccgcatgt
    30601 gaaacctcct tggttgtgaa cagtcgactc gtgtgaccat gacggccgct acacagaggg
    30661 ttgtctgtgc aacgcctggc tcagcgtatt catctcccgg cctacgccag ccaattgctg
    30721 cagatgacga ttccagcgaa taaccgggtc gaccacgtcc ccgcattgca cgcaccgccg
    30781 ggtgggacaa tcgagcccgt ctttcccgct gagaagatcc gtgcagaaat cacgaaccat
    30841 cagtccgccg caccggctgc aattcggctc ctgcgaaaag ctcgaacgat cgatccgcct
    30901 ctcgtgttgc tctacctgtc cttgcaatgc gctcgtggtt gccatggtca ctcctgccta
    30961 gttatgtcca atgtttgcat cgactatgtc taatgtttaa accattctgt ccaatgtgtc
    31021 aatagttatt atttaaaata tttgacaaac attggacatt gaacatatac ttagccgtac
    31081 caatgaatat ccacagaatt catagggttg cgaagcttac cggcctaagc cgcgatgtca
    31141 ttcgggtgtg ggagcgacgg tttgacctct taaaaccaac cagaggtgcg aaccgatatc
    31201 ggaactattc cgatgatgac gtcgcattgt tgcgatactt gaagcagcaa ttagatggcg
    31261 gagcgtcgat cggcgacctt gcgcgtctcg gccgtgaaga attgatgaat cgtgtgcggg
    31321 ccgctgcacc gcgcacgatc ccagtcgaca atacctttga gcgcctgttg cgcgaattac
    31381 tgtccgcgct ggaaccactc gatcgcgtga cgtttgaaaa acgcttgaat ggcgctgtgg
    31441 ctgtggtgcc gtttgaggag gcgctccacg gaattttgct tccgctacag gaacgggtgg
    31501 gacaactgtg gcataccggc cgtatcaatg tggccgtcga acactacgtc accagccaaa
    31561 tcaaacaaaa gctctatgcg gccatgaacc agctgcccgt ggcggagttc ggcgccaccg
    31621 tggtgattgc gtgtccaccg caagaagagc atgacctggc cgcgcttgcc gtcgcctatc
    31681 gatgccgggt tcgggattgt cgcgtctatt acctcggcgc gaatgtaccg atcagttcat
    31741 tgagcaaact ctgccgcgaa gtggcgccgg acctcaccat cctatccatc acgatcgctc
    31801 cttcggatcc gcttgcgacc gacctcgtgc aggcactgat tcacgatgtg gcaccggttt
    31861 ctaaaattat cgccggtggc cgaggagcgc tggccatgcg ccaacacttt gcagaatcag
    31921 gcatcgaact cctggaatcc ttcggcgatt tggatgagac gttggagcga ttcacccgac
    31981 gatttccgat tccaggataa cgaagggagc tcttctgatg gttgggtatc ccctcaccgt
    32041 atggtatgac ggcgcctgcc ctctgtgctc gcgcgaaatg gcctttatga aacgactcga
    32101 cagaaaacga agactccagt tctgcgactt ctccgctccc gactacaatg tcgccgccag
    32161 cggaattcct ctatccgaac tcggcgccgt gattcacgcg caatgggaag atgggacggt
    32221 catcaggagt gtcgaagtat ttcgcgcgat ctggcaggct gtcgggctgg gatttctcac
    32281 tcgaatgagc cgcctgccgc tcatcgatcc gcttgtggta caggcctatg cctggtttgc
    32341 aagaaatcgg ctctggctca ccggccgcac ggtttgcact ggaggtcagt gccggaacaa
    32401 atctgccgcc aacgcggcac ctccgtctgc ataaccgcta ggtgaagcaa ccacgttccg
    32461 cctccgtacg gttgccccga acccgcgtag cttcctcgca ctccagttct tgcacatagc
    32521 ccgtcatacg atctgtcttc ccacacgctt cttacccatg gcggtttgat gtaagacgcc
    32581 tccgacattc accgcaatcc tattgatttc acgcggcgag aattgtctta cagcagaacc
    32641 agccgacgcc ttgattatgt tgaatgccta ggcattttcc cagccaaacg gacacgctcg
    32701 act
//
LOCUS       DAOQEW010000018        31391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_311_length_31391_cov_8.810569, whole genome shotgun sequence.
ACCESSION   DAOQEW010000018 DAOQEW010000000
VERSION     DAOQEW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31391
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_311_length_31391_cov_8.810569"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /gene="serA"
                     /locus_tag="PLS35_03595"
     CDS             complement(<1..1586)
                     /gene="serA"
                     /locus_tag="PLS35_03595"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNI17769.1"
                     /translation="MKILVSDSLSKQGVEVLEKAGFTVVVKTKLPKEELLKEIKDADG
                     LIVRSGTKVTAELIAAASRLKVVGRAGSGLDNVDTPAATRRGIVVMNTPGGNTVTTAE
                     HTMAMIFSMSRRIPQATASTKGGKWEKEKFMGVELYNKVLGIVGVGQIGGYLTKLAQG
                     VGMQVMAYDPYLAPERAQKMGVSIVELDELFRRADVISVHTPLTPETKSLINAQAIAK
                     MKTGVMIANCARGGIVHEGDLCEALKSKKVAAAAFDVFEDEPVKSDNPLLALDNFICS
                     PHIGASTTEAQENVAVGIAEQIVEYFTKGIARGAVNIPSVSPELLPQLQPYLSLGERV
                     GLLQAQLLEGGLERLTVEYSGEVAGLNVAPLTIAVLKGLLTPILEDPVNYVNAPVVAK
                     ERGIEVKEVKSNDAGDFTSVIRVRVEAGKKSHQVAGTLYHRKDPRIIEIDEFKVEVVP
                     DNHLLLVLNEDRPGVIGTVGHILGDHNINIARMQCSREERGGKALLIFGLDAPLPAPV
                     LDQITNSKHILSVKVADLSKG"
     gene            complement(1615..2760)
                     /locus_tag="PLS35_03600"
     CDS             complement(1615..2760)
                     /locus_tag="PLS35_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNI17770.1"
                     /translation="MLKRYLLAPGPTPVPPEVLLAMARPMIHHRAPEFDKLFAEVRED
                     LKWLFQTRNDVLILAASGTGGMEGSVSNFLSPGDKALTINGGKFGERWTKLCKTFGAQ
                     VTELKVEWGRAIDPQAVADALKKDPSIKAVYVQASETSTAVAHDVKALAEIVKQYEDT
                     ILVVDAITALGVLDLKTDAWGLDVVITGSQKALMLPPGLAFASVSEKAWRLADKAKNA
                     AFYFNFKRERENQQKSTTAYTPAVSLILGLKEVMNILKAEGLEAVFARHAMLATAMRE
                     GVKAAGLSIFPQERPSDALTAISAPEGVDGQAVYKNLRTQYGMTAAGGQDHLKGKIFR
                     ISHMGYIDSFDVITALAAVEMVIKGLGYPVKLGSGVAKAQEIIMGKS"
     gene            3175..4476
                     /locus_tag="PLS35_03605"
     CDS             3175..4476
                     /locus_tag="PLS35_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNI17771.1"
                     /translation="MQTEPALTSEVPAVPLPDSLQPPAQEPDYLLEEEDRTAAAESEA
                     AEDPAAVASEPSTEIPPAEALLQLKPLSGTTARFADLLTPPAEVIQEAAVEQGSESDN
                     VSEYNVPIVLDPSVQGHIRFFNVAIRNRFEQWLIRLSHYRPLVDSIFSEFQLPSDLIY
                     LSLVESGFNPHAYSRARAAGPWQFMKGTAKVYGLRVDSYVDERRDPVKSTVAAARYLR
                     DLYDLFGTWPLAMAAYNAGEGKVMRALHTAQAESFSDIAKTRLIRRETKEYVPRFMAA
                     TIIAKNPDRYGFPQNDVRPHQFEEVVVRRPIHFKAIANVTGISYQELKVLNPELRRDA
                     TPPDDPEYHLKVPVGTREKVEQLLERAPTHKFPPLPMPVQVKGRHFKNETDSGHWYRV
                     RVGDSLEKIAKRFNISVKTLKSNNNLTGPTIKAGSRLVIAN"
     gene            complement(4513..5349)
                     /locus_tag="PLS35_03610"
     CDS             complement(4513..5349)
                     /locus_tag="PLS35_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17772.1"
                     /translation="MSYRIRVPAKVDPLDEAHLLTGVDRVLHVLQEQRRAVLAGLGVV
                     LVAGAVVAGVMWYDYQSTLKARELDQEATLHYLNRPADDPKKSQEQLSQAIALYKQVV
                     EQYPRSPAAPLALFHLGNAQVLANEVDAGIETYKRFMLLYGSNTSLLGLVQQRLAYAY
                     LAKGDRDQAVKAFTGILDVPGALNKDHVLFELAKIEESQSRPEGALAHYQDLMKNYPN
                     SPFTSEAAVRVKVLEVKKAPETPAAAPAPAPAASAPASQPEQPAKASTGKSAASSSKK
                     KQ"
     gene            5433..6623
                     /gene="bioF"
                     /locus_tag="PLS35_03615"
     CDS             5433..6623
                     /gene="bioF"
                     /locus_tag="PLS35_03615"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNI17773.1"
                     /translation="MFGLVKARAPMFEKHLQKLDDQHLLRRLRTIASATGPTVTLDGR
                     DIILLSSNNYLGLATHPTVVNAAIEATRRYGAGAGAARLVCGTLTPHETLERTLAGFK
                     GTEAALTFAAGYLANISVIPALIGKDELILADRLCHASLIDGCRLSGATFRVYHHRDM
                     NHLEQLLARRPAGRRTLIVTDGLFSMDGDIAPMKDLVQLAERYGAELYVDDAHGTGIL
                     GRTGRGTLEHCEVESRVPYHMGTLSKALGSAGGYVTGSATFIAYLVNTCRAFIYTTAP
                     PPATAAAATAAIQVIEQEPQRRASLWRNRERLAQGLLRLGFRLAASESPILPVIIGDP
                     DRAMKLAQALLAQGVYAPAIRPPTVPPATSRIRFTITADHTDEQIDRALIAAEQAGRS
                     INLI"
     gene            6825..7880
                     /locus_tag="PLS35_03620"
     CDS             6825..7880
                     /locus_tag="PLS35_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNI17774.1"
                     /translation="MIDISKLLTFGVQQGASDCHISAGEPPMIRLHGDLKKLDHPPLT
                     QDETHALIYDMMSDAQRKNFEEHRECDFSFDLGDIARFRVNVFVQGRGLGAVFRTIPT
                     EILPLEKLGMPPILRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNTFEGHILTIE
                     DPVEFVHKSKKCLVNQRELGVHTLSFANALRAALREDPDIILVGEMRDLDTIQLALTA
                     AETGHLVFATLHTSSAPKTIDRIIDAFPPNQQAQVRAQLSETLEAVLTQTLLKKKSGG
                     RIAAVEIMVGTTAVRNLIREGKLHQIPGIMQASQKDGMQTMDMALVDLATRGMVTKAE
                     AQSRSMNPNLFNTAGAA"
     gene            7930..>8585
                     /locus_tag="PLS35_03625"
     CDS             7930..>8585
                     /locus_tag="PLS35_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNI17775.1"
                     /translation="MDVRTLLEVMVKQESSDLYLTVDAPPIYRIHGSTHRTDAPPFTN
                     EQLESLALALMRGQQRGEFEEKMEMNLALYYKDLGRFRVNIFRQKGNVGLVFRHIKAE
                     IMTVEQLDLPPIVKDIAMTKRGLVLVVGATGSGKSTSLAAMIDHRNTVHAGHIISVED
                     PIEFVHHHKKSIVTQREVGFDTHSFGNALKNTLRQAPDVILIGEIRDTETMEAAITFA
                     "
     assembly_gap    8586..8685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8686..8925
                     /locus_tag="PLS35_03630"
     CDS             <8686..8925
                     /locus_tag="PLS35_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HNI17776.1"
                     /translation="IDEGCQTFDHVLLQLYKAGKISIEQALINADSANNLRLKIKLAG
                     LKGDDAVAALLDKNERDEKGFQIQGQMSQGGGKKR"
     gene            complement(8990..9496)
                     /locus_tag="PLS35_03635"
     CDS             complement(8990..9496)
                     /locus_tag="PLS35_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17777.1"
                     /translation="MVAPNDSSSVPPSQHNHKAIAALVAGLVLLLALVGIQNAPPPSA
                     VAPAVPNIDPNMVPLVTGEEPIQELFVRAGCVVCHRIPGIVGADGKVGPPLWLGKTGA
                     SRLADPQYRGQAQTVREYIVESVVSPGTYVVPGFPADTMPVWYGRKLTAGALDKIAAY
                     LELATDEP"
     gene            complement(9601..10452)
                     /locus_tag="PLS35_03640"
     CDS             complement(9601..10452)
                     /locus_tag="PLS35_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI17778.1"
                     /translation="MRLFSVTAVLSAIMLVGVGMASAAEPQAAASGTSAEGIKGKDGA
                     PMVLIPAGPFTMGSNDGLPAERPEHTVTLDAFYIDRYEVSMKHYRSFLVEAKHDAPPT
                     WDDEAAETVGDRPAVGMGWADAKAYCAWAGKRLPTEAEWEKAARGTDGRRYPWGQMQP
                     FVDIANYNRGVWVSEAVTLTSVSGGVEGMSVRHGLKEGGRSPYGLHHMAGNAAEWVAD
                     WYDREYYSKSPEKNPTGPETGEKKVIRGGSWSDLPVALRSSARVSAEPDFQDRAIGFR
                     CAMTPGL"
     gene            complement(10702..11196)
                     /locus_tag="PLS35_03645"
     CDS             complement(10702..11196)
                     /locus_tag="PLS35_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17779.1"
                     /translation="MSKHRGFCFTLLLLLVGVVEGCTTMPSGTSTVLPSSAFFPLDAS
                     ELKPLQAIAHAQDARMKNCHKGPACEDAYYTRALVALFENRADAITVFQELRTAMPNS
                     RYEVATVGWLNLLQDASLSSDHNKALMVQLKQEVLHNLLDRSEVATARPVKDHDRRVA
                     ELAR"
     gene            complement(11477..18793)
                     /locus_tag="PLS35_03650"
     CDS             complement(11477..18793)
                     /locus_tag="PLS35_03650"
                     /inference="COORDINATES: protein motif:HMM:NF018319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNI17780.1"
                     /translation="MSSNTTAWLNFALQQMAAESYLQDIDLHNELLVKPRLLLGNNNQ
                     FGISPTDADLAGKTRFTSVLADRFLARYDIVDHHASDATGFSATLLFDKETQQYTLSI
                     RSTEYRDEAQGGDWQRDGLPGADGEIKDYGFALAQLVSMERYWRELTAVGGKLAPDAK
                     INVTGYSLSGHLATVFTEMHSDRILQTYTFNGAGRGFVSELGQDGQPVEQTLRRMMLD
                     LEGRLLAFDPEGTQFRSGAAGNIYGDARYDVARQATLTRFPTIGTGNTQFFTIPAGVV
                     GGIPTQSEALDKVVQLVGNAETGSDVQFVANSGIHAPSQSIFIEGQPLLEGFNQQREF
                     QYGNTHSLTLLVDSFALQELFLKVDPTLEQSQIEGIFNAVSAQKADVTVLPGVIPLAE
                     GDTLEKTLDALRKVFLGNVSSTPFGRQPGDFGNLGNRDAFYQNIQQVTAALTGVSYRI
                     DSLVGQSASTMRTIALQDGPNDALGLAYRYALKELNPFVLRGMDRDTTQALYSRHNET
                     GELSLLDPNTGTGNLTSLYFEDRAAFLLKKIEVDSHSISLPSLTHFNDIELGYELGSD
                     ALPLPQVLFGGQGGDSLIGSLLFVDHLYGGQGKDQLYGNGGKDYLEGNQEDDLLDGGS
                     GADTMLGGTGDDIYIVDNIGDVVREYANSGRDEVKSSVTFTLDSQVENLTITESSARN
                     GTGNELENTIVGNSAINILSGLGGQDHLVGGGGNDILLGGTGDNDLLEGGIGFDTYIY
                     HSGDGMDRIEDSDAQGQIVFDARLLQGGIRRAGDGTNTYTSLDGQTTYVMSGTDLIVN
                     GVLIVNENFQSGQMGIQLRDVSAMPRDTGVPAGHFTDTYIGDADAEEWVPGAGAIEVY
                     ANGGNDVLHGDHAPILGAFDDLLDGGAGNDVFFGGYGDDYLIGGYGDDYAFVTDGDMF
                     LGGDGDDYAVGTANYLSFAEPIIGGGAHYADGGAGNDTLMGEMGADVLLGGDGDDVLR
                     GENRPTGWIGLAYDNSFWNRTPQAGIISLSGGNDYLDGGAGSDLLVGDGGDDILLGGA
                     GDDRLYGESDFTLTIAGDDWLDGGEGNDSLFGGAGADMLSGGDGDDLLVGDFSGDPGS
                     ADILDGGAGADELQGGGGDDILYGGIGMDRLAGFGGNDFLDGGADNDELQGGAGDDSL
                     WGGLGNDRVTGQEGDDTLFGDEGDDELQGDAGGDTLFGGEGHDALFGQDGDDLLSGEA
                     GDDLLNGGLGNDQLDGGDGIDDVQGREGDDFLVGGDGSDFLYGDGNNPTVLSLAGGND
                     TLDGEEGDDQLWAGAGQDQLFGGEGSDQLVGDAGDDQLYGEAGNDFLFGDSPFFSTQA
                     GSDVLDGGEGDDTLQGGGGEDDLQGGRGNDLLIGGSGLDTYRFNLGDGADTIQDDGVQ
                     RNRLIFGAGITAESLRLDVGPADTLVLRVGNDEDVIRVSNFGLNDPAEFHTIRQFEFA
                     DGTVLTDGELLARGFQLSSPETGGTLQGTSFDDRMQGSQVGDLLYGRGGNDVLLGGVG
                     DDVLQGDEGDDELDGGAGNDRLYGNQGFNVLRGGEGNDILESVGLGDQLLGGGGDDAY
                     HLRSMGQVITEEVNAGRDTIYLTPTESLTFQAPDNFENVRIEDDVYLDSTVQVNLVGN
                     ALDNELSGSHRLDGQAGNDTLIGTGDNTFVFGPGYGQDVVRTGLQVYAHSGLDQVQFL
                     QGVTPSNLSLERQANDLVVKVTGTADELRVQSYYESPANMVDQFLFADGAIWTSGEIE
                     NRVRTFIGVGADESFYGSAGDDTIRGMGGNDQIRANAGNDVLDGGVGNDFLEGYSGND
                     TYVFGRGYGHDTIDDQGDSADIDTLQLVDGITPGDITLQATPDFQTDALLTIKNSADQ
                     VTLAGFFQSDGMRVDRIQFSDGTMWDYSAMLAHTEGVNLVGTEDTDYFYGNVTNDTLL
                     GVGGDDVLSGGAGNDTLDGGTGADRMTGGSGNDVFMVDNINDIVMESVGQGIDTVYSS
                     LTYTLGSNLENLTLTGSAAINGTGNSLNNILTGNSAANVLTGGAGNDTYVVGLGDTVV
                     EAASAGTDTVRSDISWTLGTNLENLILTGIAAVNGTGNSLANTLTGNSAANILSGLAG
                     ADTLIGAQGDDLYIVDNVGDKITELAGEGLDTVQSSVTYTLSANVENLTLSGTSAING
                     TGNGLDNVIIGNSAANQLVGGAGNDTYVIGSGDTVVEGINAGIDTVQSSVTHTLAANV
                     ENLTLIGTGAINGTGNALNNILVGNNATNTLTGGAGDDTYVVGTGDTVVEALNAGLDT
                     VQSTVTWTLGANLENLTLSGAAAINGTGNTLANSLIGNAGNNVLMGGDGNDTLSGGKG
                     NDVLNGGTGNDLFQVARGDGQDTITDASGSSDRLSFASGINPLDLMLSQSANDLRIAV
                     YGSTDQVTITNWYAGAANQIETVQAGNGQQLMSTQVNQLIQAMAGFTQQTGLSWDQAI
                     AQRPQDVQQILAANWH"
     gene            complement(18783..19784)
                     /locus_tag="PLS35_03655"
     CDS             complement(18783..19784)
                     /locus_tag="PLS35_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17781.1"
                     /translation="MKQNRRMLSLFVIAGLLSLALIAPTGAGLFGKDETVGERFRKAI
                     EKIKQSCESRKLARGEVCGEVAKLKPADPLATEEGRFAHSIKIPNPVPEDSGYKPGMT
                     SQEYFEHLCKTEAGEFIYKTAENVEGLYMMRPREHANDYMQEHLYAMEDPYGYMDAES
                     QDPEFFFVGPLRYKFFEHPLAPRKKPSWGKSFLDNSYFTAPSDRAYHERYLGSNGKDP
                     LTMIKEFDSVRKARYGYTWRGIVRPHDREMGIAGGELIVLDMETGEVLGVRRGYIRTG
                     SVRNNLTGVWWLTGQACPYYQHRGGRSRDFDFSYWFIGKVVKPINYEMNVRELMNVK"
     gene            complement(19837..21231)
                     /locus_tag="PLS35_03660"
     CDS             complement(19837..21231)
                     /locus_tag="PLS35_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNI17782.1"
                     /translation="MMNALQLKLAGWRTIGRVGSGQSSHAAAQGEAIEFLPAILEIQD
                     APPSPIGRAILWTIMLAFASAVTWSSLSQVDIVAVAPGKIIPSGYSKTIQPFETGVIA
                     AIHVQDGQSVKRGEVLIELDATQNSADRDRASNEYRAALVEAARLHALIAGHSTFTAP
                     SESDPEFVLLQQQLLRDQMVEYAARVDAARHLIGQRKAAVDAAKDNLRRLEATVPMEN
                     ERAAAYRALLAQQYVSKMDYLQFEQQRIDKAQEWAGQKSKLRQDQAALAEAEQNYQVL
                     ISEFQQSKQAELSAMETKATSLVQEVRKAGQKTELQSLVSPIDGVVQQLAVHTVGGVV
                     TPAQTLLMVVPQDHPVEVEAQLENKDIGFVKEGQPVELKIETFPFTLYGTIPGKVLTV
                     SDDAVPLDKEKGGLVYVSRVSMDRATMQVEGKHVHLTPGMAVTVEIKTGQRRVIEFLL
                     SPLLKSMKESLRER"
     gene            complement(21231..23393)
                     /locus_tag="PLS35_03665"
     CDS             complement(21231..23393)
                     /locus_tag="PLS35_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNI17783.1"
                     /translation="MDVPTSHLESCSTAPPRPDTGLLCLLLIARFHDLPIDSGQIQHQ
                     FGLSGHMLTTNALVRAAKHVGLKAGLVSSSWGELAGAPLPAIAQRTDGTYVVLAKVQG
                     EKALLQDPLKERPHILSKEEFVACWSGSLLLFTKRSGLRVEDVSFDMTWFIPAVLKYR
                     RLLGELVLASFFLQVFALLTPLFTQVVIDKVLVHKGFTTLHVMAVGMLALALFDALLG
                     GLRTYLFAHTTNRIDVGLGAQLFRHLLALPLAYFEARRVGDTVARVRELEQIRQFITS
                     NSVTVVLDVVFTIVFLGVMWIYSPTLTLVVMASLPLYALLSVLITPAIRARLHEKFNR
                     GAENQSFLVEAVSGIQTVKALAVEPPLQRRWDEQLAGYVAASFRATSLITVAGQIATC
                     IQKSTTIAIMWVGAYQVIDGALSIGELIAFNMLSGQVTGPLLRMVNLWQEFQQVGISI
                     QRLGDVLNAKLEPSYNPNRTTLPQIAGQIVFDDVAFRYRVDGPLVLQRVSLTLHPGQV
                     VGVVGRSGSGKSTIAKLMQRLYVPERGRVLVDGVDLAQIDPAWLRRQVGVVLQENFLF
                     NRSVRDNIALTDPGLPMDRVMHAAKLAGAHEFILELTDGYDTVVGEHGCALSGGQRQR
                     IAIARALVANPRILIFDEATSALDYESEAIIQRNMAQICKGRTVIIIAHRLSTVRPAH
                     KIYVIDRGQLVEQGTHDELLSRDGMYTRLHQHQQGRAA"
     gene            complement(23415..23750)
                     /locus_tag="PLS35_03670"
     CDS             complement(23415..23750)
                     /locus_tag="PLS35_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17784.1"
                     /translation="MAVGSSPKQNGDKAVAVNVRLQSSTEADQPILANYLHASLAQGL
                     AYIDFGFLEPSLIGAVAQRAQNGEAMPKQLVGSRAVRVALPLDAVIRLHQQLQQMVVS
                     LQPAKSKLS"
     gene            24108..24692
                     /locus_tag="PLS35_03675"
     CDS             24108..24692
                     /locus_tag="PLS35_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNI17785.1"
                     /translation="MQKVLMLQAGWRTLCPVTALCLSLSACAGVSTTDTLPTVASVDL
                     ARYAGTWHEIARLPMWFQRHCVDSKATYTVRNDGAIGVRNECVTDTGGHDEVEGVATV
                     VDPGTNTRLRVVFDNFFARLFGSSREGNYWILALDADYQTAMVGTPDRRYLWILSRSR
                     QLDETAYTRLVEQARRLRFPVSELIRPHRSAATS"
     gene            complement(24784..25689)
                     /locus_tag="PLS35_03680"
     CDS             complement(24784..25689)
                     /locus_tag="PLS35_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNI17786.1"
                     /translation="MWALLKSLATQTIAKWSDEPGPRFAAALAFYTALTIVPLVVLTI
                     MVSVSTVGDESMVGGLQRQIGELIGESAANGLFHIIAQWRSAGSPLVNGVVALGVFVI
                     GALKVMDQLRDALDCIWGLQPKHPPGMGDRLKRRLASLSGLFGIAFILLASLTLSAWT
                     GIAIQFVLETTGMPGRLVQGIGLLMSFGVVSTLFAMIYKWVPQAQVAWPDVWVGAGIT
                     AILYLLGSKLLVVYLAHNTLVAFYGAAGSLVLVLLWAYYASQVFLFGAAFIAVYATHH
                     GSQVKPTEETVVVHVASQGESETEG"
     gene            complement(25847..26071)
                     /locus_tag="PLS35_03685"
     CDS             complement(25847..26071)
                     /locus_tag="PLS35_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17787.1"
                     /translation="MDKAQNLASAARANLNSEMQGYADALQRIIDLARNGAMLGHDHA
                     YDACCKIEAIATEALQRPTTPHRPDDKPTG"
     gene            complement(26232..26567)
                     /locus_tag="PLS35_03690"
     CDS             complement(26232..26567)
                     /locus_tag="PLS35_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17788.1"
                     /translation="MMKVPTYVSLVLCIAVLGGCNYSDGDRTGYLQKLSRKGWVCKTY
                     EGELAMTTVPGVAPVLWNFSVWDDDVAKKLDGQMGKRVVLHYREFRYVPTTCFGETSY
                     FIDRAEVSE"
     gene            26776..27234
                     /locus_tag="PLS35_03695"
     CDS             26776..27234
                     /locus_tag="PLS35_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17789.1"
                     /translation="MIATLATALLVVLATVPNGHAVAQSSQRDVFDERDTRVFKAVGC
                     TQDKQPAEALYYIVASRSDLSAGVPSPSSQFMKDEVEQNWQRIASQLTMEQVTEERFT
                     DTYHALLNDMIPRLQRAVEEKSGVSIAVSEVNSRPMDRTKDQDVPTCSSQ"
     gene            27502..27654
                     /locus_tag="PLS35_03700"
     CDS             27502..27654
                     /locus_tag="PLS35_03700"
                     /inference="COORDINATES: protein motif:HMM:NF023180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNI17790.1"
                     /translation="MSTILLIILILLLIGALPSWPYSSGWGYYPSGGIGLILLIVLIL
                     ALTGRV"
     gene            complement(27761..28081)
                     /locus_tag="PLS35_03705"
     CDS             complement(27761..28081)
                     /locus_tag="PLS35_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17791.1"
                     /translation="MSLKIDVTTVARVLLRDGSWYEVIPGSLTMDTFQWTDREGITIA
                     SVDLEALPPMGIRFKTRGMSGWLSCPLTSIVAVHYEPIELEDPLAVELPSLLSDELDR
                     PSVQ"
     gene            complement(28565..>29158)
                     /locus_tag="PLS35_03710"
     CDS             complement(28565..>29158)
                     /locus_tag="PLS35_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI17792.1"
                     /translation="DVRMPGNSQLEFLETVARRHPDLPVVVVTGYPSVGTVIESFRLS
                     IVDYLIKPVDLNELKKAVDRGLRRRSIARAVQDAMHETAKMAGIFERLGRSMNEFGGR
                     GQQVEWTAQEYMAQAMGHISQLSALVGRTLEASRTGQPQAPTDICAVMKCPRLDRYEA
                     AIEEAIQVMEHTKSSFKSKELGTLRQHLEDVLREGRR"
     assembly_gap    29159..29258
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29259..>31391)
                     /locus_tag="PLS35_03715"
     CDS             complement(<29259..>31391)
                     /locus_tag="PLS35_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17793.1"
                     /translation="QVIGKTDHELFPQDIADSYRQHDCKVLHTGEVMSFEEDALLNND
                     PRFYVVRKFPLRDEGGRIIAVGGVATDMTARLQAEVARHEAQERLDLVVAATQTGIWD
                     WDMQANTMYYSPRWKSSLGYEEQEISNSPSEWETRLHPDDKARAFALVDDYLSGRIAS
                     YELQHRLRHKDGTYRWIHTCAVLQRDEEGRPRRMTGSHVDITERKRAEEALSRSESTL
                     RSFFESGVMMMGVVELHDGDILHLSGNRCVASFFGTTSELMQGQLASKLGAPPDTIAL
                     WHRHYEMSRESAQSVRFEYPHSDPTSGTERYLSVSVCWIGTGPSERHRYSYVAEDVTE
                     ARRTKTALVETAERYRGVVTALAEGIVLQNAQGRIIACNPSAEQILGLTAEQMMGRTS
                     VTSEWRAIHKDGTPFEGEQHPAMVTLHTGQPCADVIMGVCRPGGEQRWLSINTQPLRE
                     PHAERPYAVVASFHDITERRRAEQALVEVQTQLEQRVRERTARIHELEAQRSQAEKVA
                     ALGHLAADIAHEINNPLAGIKNAFHLVKQGVSPDHRHYRFVELIDREIQRLAAIVKKM
                     YTLYQNGASGQWQATDVSDLLQNVGTLLDYKLVSRRIQLRTEIELTCPVVTLPRADLF
                     QVLLNLVQNAIDASPQDMEVVLKVARHGDTLHWSVMDHGPGITPEVFPHIFEPFFTTK
                     SAGGPQGLGLGLSVSRGLVRAMGGRIEV"
BASE COUNT         6991 a         9417 c         7882 g         6901 t
ORIGIN      
        1 cctttcgaca ggtcggcgac ctttacggaa aggatgtgct tgctgttcgt aatttgatcg
       61 agcaccggcg caggcagcgg cgcatccagc ccgaaaatca agagggcttt gccgcctcgc
      121 tcctcacgtg agcactgcat acgcgcgata ttgatgttgt ggtcgccaag aatatgtccg
      181 accgtcccga tcactcccgg acgatcctca ttgaggacca ataacaaatg gttgtccggg
      241 accacttcga ccttgaattc gtcaatttcg atgatacgcg gatctttgcg gtgatacagc
      301 gttcccgcca cttgatgaga ctttttcccc gcttccaccc gcacgcgaat cacgctcgtg
      361 aaatcgccgg catcgttgct cttgacctct ttcacttcga tcccgcgctc tttggcgacg
      421 accggcgcgt tcacatagtt gaccgggtct tcaaggatag gcgtcaggag ccctttcaag
      481 accgcaatcg tcaacggggc gacattcagt ccggccacct caccactata ttcgacggtc
      541 aaccgctcaa gtccgccttc caataattga gcttgcaaca aacctacacg ctctcccaat
      601 gagagatagg gctggagctg cggcaagagc tcgggagaca cagagggaat gttgacggcg
      661 ccacgggcga tccctttggt aaagtattcg acaatctgct cggcgattcc caccgcgaca
      721 ttctcttgcg cttcagtcgt agacgcaccg atatgcgggg agcaaatgaa attgtccaga
      781 gccaggagag gattgtccga tttcaccggc tcgtcttcga acacgtcgaa cgccgctgcc
      841 gccaccttct tcgatttcaa tgcttcgcag agatcccctt cgtggacgat gccgccacgg
      901 gcgcagttgg cgatcatgac accggttttc atcttcgcga tagcctgtgc gttgatgagc
      961 gacttggttt caggggtcag tggagtgtgg accgagatga catcggcccg gcggaacaat
     1021 tcgtccaatt ccacgatgga tacgcccatt ttctgcgccc gttcgggtgc gagatacggg
     1081 tcataggcca tcacctgcat gccgacgccc tgggcgagct tggtaaggta tccgccgatt
     1141 tgtccgacgc cgacgatccc cagtaccttg ttatacaact ccacgcccat gaacttttct
     1201 ttttcccact tcccgccctt ggtcgaagcg gtcgcttgcg gaatacggcg cgacatagaa
     1261 aaaatcatgg ccatcgtatg ttcggcggtg gtcacagtgt tgccacccgg cgtgttcatg
     1321 accacaatgc cgcgacgggt tgcggcgggg gtatcgacat tatctagccc ggaaccggct
     1381 cggccaacta ctttcaggcg gctcgccgca gcgatgagtt ctgctgtcac tttcgtcccg
     1441 gagcgaacga tcaacccgtc cgcatccttg atttccttca gcagctcctc tttgggcagc
     1501 ttggttttca ccacgaccgt aaagccggcc ttctccaaca cctctacacc ctgcttcgaa
     1561 agactgtcgc tgaccaagat tttcattgtc gtggcgcctt acctgaaggt gagtctagga
     1621 ctttcccata ataatttctt gagccttggc aaccccgctc ccaagcttca cgggataccc
     1681 caaccctttg atgaccattt ccacagccgc caaggccgtg atgacgtcaa agctatcgat
     1741 atatcccatg tgcgagatac ggaagatctt gcctttcaga tggtcctgac cgccagcggc
     1801 cgtcatacca tactgcgtgc gcaggttctt gtagaccgcc tgaccgtcca caccttccgg
     1861 ggccgaaatg gccgttaatg catcgcttgg ccgctcctgg gggaaaatag aaagtcccgc
     1921 cgccttgacg ccttctcgca tggccgtcgc caacatcgcg tgacgggcga agacggcttc
     1981 caggccctcg gccttcaaaa tgttcatcac ttccttcagg cctaagatga gggagaccgc
     2041 cggcgtatag gcggtggtgc ttttctgctg attctcccgc tcccgcttga agttgaaata
     2101 gaaggccgcg ttcttcgcct tgtcggcgag acgccaggct ttctcactca cgctggcaaa
     2161 ggccaatccc gggggcagca tcaaggcctt ctgcgatccg gtgatgacga cgtccagtcc
     2221 ccaagcatcg gtcttcagat cgagcactcc cagggccgta atggcatcca ccacgagaat
     2281 ggtgtcttcg tattgcttca caatttcggc cagggctttg acgtcgtgtg cgacggccgt
     2341 agaggtttcg ctcgcctgca cgtacacggc tttaatcgag gggtctttct tcaacgcgtc
     2401 ggcaacggct tgggggtcga tggcgcgccc ccactccacc ttcaactccg tcacctgggc
     2461 gccaaacgtc ttgcagagct tcgtccaccg ctcaccgaac ttgccgccgt tgatcgtcag
     2521 ggctttgtcg ccaggagaga ggaagtttga aaccgaccct tccatgccgc cggtgccgga
     2581 agccgcgagg atcaaaacat cgttgcgcgt ctggaacagc catttcaggt cctcacgaac
     2641 ctcggcaaac aatttatcga actcgggtgc ccggtggtga atcatcggtc tggccatggc
     2701 cagtagcacc tccggtggaa caggggtggg gccgggtgcc agcaaatacc gtttgagcat
     2761 gggacgatcc tcctcgctgt acggaactaa caatgtcagg gatttagaag gggcgtacgc
     2821 taccatagcc ccccagaggt gtcaaggtaa gttcccagga agatgcccgt cttttcagcg
     2881 acttcactgc ctttaccgga gagttgcttt caaaatgttc cggcggacct gatgctgaga
     2941 tcctgaaagg tttcgttcgc ccagcaactc aactccctca atttcttgac aagaactcac
     3001 ctcctcgata ggctacgatt cgagagtcgg tcacctccct tccctgaacc gtctagtagg
     3061 taccttatgc atgcacgttt ttccagcgct ggcgttccgc ccctttccct tcttttcgca
     3121 tgtctgctct ctgtttcttc gcctggttct gcaggcgcgg aatcgccgat tcccatgcaa
     3181 acggagcccg ctctcacatc cgaagttcca gcagttccgt taccagatag cctccaaccc
     3241 ccggcgcaag aacctgacta tctcttagag gaagaagatc gcacggccgc cgcggaaagt
     3301 gaggcggccg aggacccagc tgcggtcgcg agcgaaccgt ccacggagat tcctccggcc
     3361 gaagccctgc tccaactcaa gccgctcagc ggtaccaccg cgagatttgc cgatttgttg
     3421 acccctccgg ctgaagtcat ccaggaagcc gccgtggagc aagggtcgga atcggacaat
     3481 gtttccgaat acaacgtccc gatcgtgctc gacccttccg tgcaaggtca tatccgcttc
     3541 ttcaatgtcg cgatccgcaa ccgattcgag cagtggctga tcagactcag ccattaccga
     3601 ccgctggttg atagcatttt ctctgaattc cagctgccga gcgatctgat ctatctgtca
     3661 ttggtagaaa gcggtttcaa tccccacgcc tattcacgtg cccgggctgc cggcccctgg
     3721 caattcatga aaggcacggc caaggtctat gggttgcgag tggacagtta tgtggatgag
     3781 cgccgggatc cggtgaagtc caccgtggca gctgcgcgct acctgcgcga cctgtacgat
     3841 ttattcggca cctggcccct ggcgatggcc gcgtacaatg cgggagaggg caaagtgatg
     3901 cgggccttgc acacggccca agcagaaagc ttttcggaca ttgcgaaaac ccggctcatc
     3961 cggcgggaaa cgaaagaata cgtgcctcgg tttatggccg cgaccatcat cgctaagaat
     4021 ccggaccggt atggcttccc ccaaaacgat gtccgacctc accagttcga agaggtggtc
     4081 gtgaggcgcc ccatccattt caaagccatc gccaatgtca cggggatttc ctaccaggaa
     4141 ctgaaggtgc tcaacccgga attacggcgc gatgccacgc ctccggatga ccccgaatat
     4201 catttgaagg ttcccgtagg caccagagag aaggtggaac agttattaga acgggctccg
     4261 actcacaagt ttccgccgct gccaatgccg gttcaggtca aaggccgaca tttcaagaac
     4321 gagactgatt ctggacattg gtatcgcgtc cgcgtcggcg actcgctgga gaaaatcgcc
     4381 aaacggttta acatctcagt caaaacgctc aagtccaaca acaacctgac cggtcccacc
     4441 attaaggcag gaagccgcct ggtcatcgcc aactaggtcg acccgtctcc cgctgtaagg
     4501 ggaaagacca ggctactgct ttttcttgga agacgaggca gccgacttgc ccgttgaagc
     4561 cttggcaggc tgttcaggct gagaagcagg ggcgctggct gctggggccg gtgcgggagc
     4621 tgccgccggg gtttccggag ctttcttcac ttccaacact ttcacacgta ccgcagcttc
     4681 actggtgaag ggcgagttgg gataattctt catcagatcc tggtagtgtg cgagggctcc
     4741 ctccggacgg gactgcgact cttcaatttt tgccaactcg aacaacacat gatccttgtt
     4801 gagggctccg gggacgtcga gaatgcccgt gaaggccttc accgcctgat cgcgatcacc
     4861 cttggctagg taggcatagg ccaaccgttg ctggaccaat cctaagagcg aggtgttgga
     4921 accgtacaac aacatgaacc gtttgtaggt ttcgataccg gcatccactt cattcgccaa
     4981 aacctgcgca ttccccaaat ggaacaaggc caagggcgca gcagggcttc tcggatattg
     5041 ctccaccact tgcttataga gcgcgatagc ctgggacagc tgttcctggc ttttcttcgg
     5101 gtcgtctgcg ggccgattga gatagtgcag cgtggcttcc tgatcaagct ctcgcgcttt
     5161 taatgtggat tgatagtcgt accacatcac accggcgacg accgctccgg caaccaacac
     5221 cacccccagc cccgccaaga cggcgcgccg ctgttcctgc aatacatgaa gaacccgatc
     5281 aacgccggtc aacaggtggg cttcgtcgag cgggtcaact tttgcaggaa ccctgattcg
     5341 ataactcatg gtcgaacaat ggtctcggtt ggaaaagacg gtcgtttctc agagcctcgc
     5401 gccttatact agggaccact cagccttgtc aaatgtttgg cctcgtcaaa gcccgggcac
     5461 ccatgttcga aaaacatctc cagaaactgg atgatcaaca tctcttgcgc cggcttcgca
     5521 cgatcgcctc ggcgaccgga ccaacggtca cattggacgg ccgtgacatc attctgctct
     5581 cctctaacaa ttatctgggg ctggccacgc atccaactgt ggtgaatgct gccatcgagg
     5641 caacccgccg atatggagcc ggcgctggcg cggcacgcct ggtttgcggg acgctcacac
     5701 cccatgagac gcttgaacgc accctcgccg gattcaaagg cacagaagca gccctcacct
     5761 ttgccgccgg gtacctggcc aatatcagcg tgattccggc cctgatcgga aaagacgagc
     5821 tgatccttgc cgaccgtctc tgtcacgcca gtctcatcga cgggtgccgt ctgagcggag
     5881 ccacctttcg tgtgtaccat catcgagaca tgaatcatct ggagcaactg ctcgcccgtc
     5941 gtcccgccgg tcgacgtaca ctaatcgtca ctgatgggct attcagtatg gacggagaca
     6001 ttgccccgat gaaggatctc gtacagctgg cggaacggta tggtgcagag ctctatgtcg
     6061 acgacgcgca tggcacagga attcttggac gcaccgggcg cggcaccctc gaacattgtg
     6121 aggtcgaatc gcgtgtgccc tatcacatgg ggaccttgag caaagcactc gggagcgcgg
     6181 ggggatacgt gaccggttca gccacattca tcgcctattt ggtcaacacc tgccgcgcgt
     6241 tcatttacac caccgctccg cctccggcca ctgccgctgc ggcgaccgct gcaatccagg
     6301 tgatcgagca ggaacctcag cggcgcgcca gcctgtggcg caaccgcgaa cgactggcac
     6361 aaggtctcct tcggctggga ttccgcctcg cggcatcgga gagtccgatc ctgccggtga
     6421 ttatcggcga tcccgatcgt gcgatgaagt tagcgcaggc cctccttgca caaggcgtgt
     6481 atgcccccgc cattcgtcct ccgaccgtgc ctcctgctac cagtagaatt cgcttcacca
     6541 tcacggcgga ccataccgat gagcagatcg atcgcgcact gattgctgca gaacaggccg
     6601 gtcgatccat caatctgatc tgacctgacg actccccagt catctcccga tgaccacaag
     6661 agttagggcc ctagcaacgg gcatttcgtc gtcgcctctt ctggatattt tcttgatttc
     6721 tcaccctcct atggcatgat cgaaatggtc gagcgagcct tgatcttcta gacataacta
     6781 cgttcgtttt atccgcgcac attctctttc aatggagcga cccgatgatt gacatctcca
     6841 aactgttgac ctttggtgtc cagcaaggtg cctcggactg tcacatcagc gccggcgaac
     6901 cgccgatgat ccgtctgcat ggtgacctca aaaagctcga ccatccaccc ctgacacagg
     6961 atgaaactca cgccctcatc tacgacatga tgagcgatgc ccagcgcaaa aactttgaag
     7021 aacatcggga atgtgacttt tccttcgacc tcggcgacat cgctcgattc cgcgtgaacg
     7081 tctttgtcca gggccgcggg ttaggtgctg tcttccggac cattccaacg gaaattcttc
     7141 cgctcgaaaa actcggcatg ccgcccattc tccgacaact gtgcgaccgt gagaagggcc
     7201 tgattcttgt caccggcccc accggatccg ggaaatccac caccctcgcc ggtatgatcg
     7261 actatctgaa caacaccttt gaaggccaca ttctcaccat cgaagacccg gtggagtttg
     7321 tgcacaaatc gaaaaagtgc ctggtgaacc aacgcgaatt aggcgtacac accctgtcat
     7381 tcgccaatgc actccgtgcc gcactccgcg aggaccccga tatcatcctc gtcggcgaaa
     7441 tgcgggacct ggatacgatc cagttggcgt tgaccgctgc cgaaaccggg cacttagtct
     7501 ttgcgactct gcacacgtct agcgccccga aaaccatcga tcgcatcatc gatgcgtttc
     7561 ctccgaacca gcaagcgcaa gtccgcgcgc agctgtcgga aaccttggaa gcggtgctaa
     7621 cacagacgct gctcaaaaag aaaagtggtg gccggattgc agccgtggaa atcatggtcg
     7681 gcaccactgc ggtgcgtaac ctgatccgcg aaggtaagct gcatcaaatt cccggcatca
     7741 tgcaggcgag ccagaaagac ggcatgcaga ccatggacat ggccctggtc gacctcgcca
     7801 cgcgcgggat ggtgaccaaa gccgaggccc agtctcgcag catgaatccc aaccttttta
     7861 acacggccgg cgcggcctaa cgccccattc acggtattcg tctaccgata gccgcataag
     7921 aggcatctca tggatgttcg cacactcctc gaagtgatgg tcaaacagga atcgtccgac
     7981 ctgtatctca ccgtcgatgc gccaccgatt tatcgcatcc atggctcgac tcatcgaaca
     8041 gatgcgccac cgtttaccaa tgaacagttg gagtcattgg cactggctct gatgcgcggc
     8101 cagcagcgtg gcgaattcga agagaaaatg gagatgaacc tcgccctgta ttacaaagac
     8161 ctcgggcggt tccgcgtgaa catcttccgg caaaagggaa acgtagggtt agtgtttcgt
     8221 cacatcaagg cggagatcat gacggttgag cagttggacc tgccgcccat cgtcaaagat
     8281 atcgccatga cgaaacgcgg gctggtcctt gttgtagggg ccaccggctc cggtaaatcc
     8341 acctcactcg cggcgatgat cgatcacagg aacaccgtgc atgcggggca cattatcagc
     8401 gtggaagatc cgattgagtt cgtccatcac cacaaaaaat cgatcgtcac ccaacgggaa
     8461 gtcggtttcg acacgcattc gttcggcaat gcactgaaga acacgttgcg ccaggccccg
     8521 gatgtgattc tcatcggcga aattcgtgac acggaaacca tggaagcggc catcaccttt
     8581 gcggannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattga tgaagggtgc
     8701 cagacctttg accatgtgct cttacaactc tataaggccg ggaaaatcag cattgaacaa
     8761 gccttgatca acgctgatag cgccaacaat ctccggctca agattaaact ggcgggatta
     8821 aaaggtgatg atgcagtggc agccctgctc gacaaaaacg aaagagatga aaagggcttc
     8881 caaatccagg gacagatgag ccaagggggc ggcaagaaac gatgatgacg gacaggagtg
     8941 agctactgct ctaggcctca cgcaagcaac caggtgtatg aagcccaggc tacggctcat
     9001 ccgtagcgag ttcgagatac gcggcgatct tatccagcgc tcctgcagtc aatttcctcc
     9061 cataccagac cggcatcgtg tccgcaggaa aaccaggcac cacataggtg cctggcgaca
     9121 ccactgactc aacaatatat tcgcgaaccg tttgcgcctg cccccgatat tgtggatctg
     9181 ccaggcgaga ggcaccggtc ttgcccagcc agagcggagg gccgaccttt ccatcagccc
     9241 ccacgatacc cggaatccga tggcaaacca cacaaccggc gcgaacaaag agctcttgaa
     9301 taggctcctc ccccgtcacc aacggcacca tattcgggtc tatgttcgga acagccggag
     9361 ccactgcgga cggtggcgga gcattttgaa ttcccaccag agccagcaac aacaccaagc
     9421 cggcgaccaa tgccgcaatc gctttatggt tatgctgtga gggcggaaca gaggaagaat
     9481 cgtttggagc gaccatacac cggcaccatg aaccttacac gcacattgaa caaacacgct
     9541 actgcgtcaa cagatgaaga aagactacta gtctcggcaa cgcgtgaata atagccgatg
     9601 ctatagtccc ggagtcatgg cgcaacgaaa gccgatcgct cgatcctgaa aatccggttc
     9661 cgccgagact cgcgcagatg atcgtaacgc caccggcaga tctgaccagg accctccacg
     9721 aatcactttc ttctctccgg tttccgggcc agtgggattc ttttcaggac tcttcgaata
     9781 gtattcccga tcgtaccaat ccgccaccca ttcggcggca ttccctgcca tgtggtgaag
     9841 gccataggga ctgcgcccgc cttctttcaa gccatggcgt acactcatcc cctcgacacc
     9901 gccactcact gaggtaagcg tcaccgcctc gctcacccat accccgcgat tgtaattcgc
     9961 aatgtcgacg aagggctgca tttgtcccca cgggtatcgc cgcccgtcgg taccgcgcgc
    10021 cgccttttcc cattcggcct ctgtcggaag acgctttcct gcccaggcac aataggcctt
    10081 tgcatcagcc cagcccattc ctacggccgg acgatcacca accgtctctg cggcttcatc
    10141 atcccaggtc ggaggagcat cgtgcttcgc ctctacgagg aacgaccgat agtgcttcat
    10201 ggagacttca tagcgatcga tataaaacgc gtcgagagtc acggtatgtt ccggtcgttc
    10261 agcgggaagc ccgtcgttac tgcccatcgt aaatggcccg gccggaatca gcaccatggg
    10321 cgcgccatct ttgcctttga taccttccgc agaagtaccg cttgccgcgg cctgaggttc
    10381 ggcagccgag gccatcccta cgccaacaag catgatggct gacaacacgg ccgttacact
    10441 gaagagtcgc atacattcct cccatctgcg atagatccag aggcaggtca ccacgcccgg
    10501 caaagtgtag ccgtgcgggc agccaaactc aactgcggca aaatccgaga cacggttgaa
    10561 agtgatgtcg ctgtcggcaa ggtgagatag tggttgggaa aagcgagtcc cggagaaagg
    10621 cgatacaacc ggcggcggca tgttggccgg caagaaggtc cttgccggcc tggtgggaaa
    10681 gaggaaggag tggcgggata gttaccgggc gagctctgca acacggcggt cgtgatcttt
    10741 taccggcctc gccgtagcca cttcagaccg gtctaagagg ttgtggagga cttcttgttt
    10801 caactgaacc ataagcgcct tgttatggtc tgaggatagc gatgcatcct gaagcagatt
    10861 gagccagccc accgtcgcaa cctcataccg gctgttcggc atggccgtgc gtaattcctg
    10921 aaacaccgta atggcatccg cccgattttc aaacaacgcc accaacgctc gcgtgtagta
    10981 ggcatcttca caggctgggc ccttatggca attcttcatt ctcgcatcct gcgcatgagc
    11041 aatggcctga agcggcttga gctccgaggc atcgaggggg aaaaatgcgc tactgggtag
    11101 gaccgtggag gtgcccgatg gcatcgttgt gcatccttcg accacaccaa cgagcagcag
    11161 gagaagggtg aagcagaacc ctcggtgttt cgacatcgta agcctcctca actcgcgctc
    11221 gccagatggg ggcgcgatga tattgcgatg tacttggctt gcagggatgt tctgaatgta
    11281 ccatcgcgct tcggttcagc cattacacgg aggctgtacc tctttggtat ggtgaatctg
    11341 cgcggggcac ggccaggtca caggcactca ttcgaacgaa ataagtgggg atcgggcctg
    11401 aacaatctca ggaggaggaa gacaacggtg ataagaatcc tccgccccgc cttgtatgcg
    11461 gggcgaaggc tcggccttaa tgccagtttg cggcaagaat ttgttgcacg tcctgtgggc
    11521 gctgagcaat ggcctgatcc caactgagcc cagtctgttg ggtaaatccc gccatagcct
    11581 gaatgagttg attcacctga gtactcatca attgttgacc attgcctgct tggaccgttt
    11641 caatctggtt ggctgcaccg gcataccagt tggtaatggt cacctgatcg gtcgaaccat
    11701 agacggcaat gcgcaaatca ttagcgctct gactcaacat aagatctagt ggattgatcc
    11761 ccgacgcaaa actcaagcga tcgctgctcc cgctggcatc tgtaatcgtg tcttggccat
    11821 ctccgcgagc cacctgaaac aggtcgttcc ctgtcccacc attgagcaca tcatttcctt
    11881 ttccgcccga caacgtatca ttgccgtcgc cacccatcaa aacgttgttg ccggcgttac
    11941 cgatcagaga attagctaga gtattgcctg ttccgttgat cgcggctgct ccgctaagag
    12001 taaggttctc aagattcgca cctaaggtcc aggtcaccgt gctttgcacc gtatcaaggc
    12061 cagcgttcaa agcctcgacc actgtgtcgc ccgtccctac cacatacgtg tcatccccgg
    12121 cgccgccggt cagggtgttg gtggcattat tgccgacgag aatattatta agcgcgttac
    12181 ctgtaccgtt gatcgcaccg gtgcctatga gggtgagatt ttcgacattg gctgcgagcg
    12241 tatgagtcac ggaactctga acagtatcga tcccggcatt tattccctcg acgacggtat
    12301 cacccgagcc gatgacatac gtgtcatttc ccgcacctcc gactaactga ttggcagcac
    12361 tattcccaat tatcacgttg tccagtccgt tgccggtccc gttgattgcg gaggtgccgc
    12421 tcagcgtaag attttccaca ttggcactca acgtgtaggt cacagaactc tgaaccgtat
    12481 caagcccttc accggctagt tcagtgatct tgtcgccgac gttgtccaca atgtaaagat
    12541 catccccctg agccccgatg agtgtatctg ccccagccag gccactcaga atattggccg
    12601 cactgttacc ggtcagggtg ttggcgaggc tattgccggt cccgttaacc gcagcaatgc
    12661 cggtcaagat gagattttcg agattcgtgc ccagcgtcca actgatatca gacctgacag
    12721 tgtcagtacc cgcactcgcc gcctctacaa cggtgtcgcc aaggcccacc acgtaggtgt
    12781 cgtttccggc cccaccagtc aggacatttg ccgcactatt tccagtcaaa atattattca
    12841 gagaattgcc agtaccattg atggcagcgc ttccggtgag ggtcagattt tccaggttcg
    12901 aacccagcgt ataggttagg ctactgtaca ccgtatcgat accttgaccg actgattcca
    12961 tcacgatgtc gttgatattg tcgaccataa acacatcatt tccggaacca ccggtcatgc
    13021 gatctgcgcc agtgcctcca tcaagcgtgt cattgcctgc gcctcctgag agcacgtcgt
    13081 cgccacctac ccccaacaat gtatcgttcg tcacatttcc gtaaaaatag tccgtgtctt
    13141 cggtgcctac cagattgacg ccttccgtat gggccaacat ggcgctgtaa tcccacatcg
    13201 tcccatcgct aaactgaatt cgatcaacgc gcatcccatc ggactggaaa aatccagcga
    13261 gcgtcacttg atccgcacta ttcttgatgg tgagaagagc gtcggtttga aaatcaggcg
    13321 ttgcctgcaa ggtgatatcg cccggtgtaa ttccgtcgac aagttgaagg gtatcgatat
    13381 cagcgctgtc accttgatca tcaatagtat cgtgcccata accgcggccg aacacatagg
    13441 tgtcgtttcc gctatatcct tccaggaagt cgtttccgac gcctccgtca agcacatcat
    13501 tcccagcatt ggctcggatc tgatcattcc cccccatgcc tcggatcgta tcgtcaccag
    13561 ctgaaccata gaatgattcg tcagccccga cgccgatgaa ggttcgtacg cgattttcaa
    13621 tctctccaga ggtccagatg gctccatccg cgaagaggaa ttgatctacc atgttcgctg
    13681 gagactcgta ataagactgt acgcgtaact cgtcggcagt tccggtaacc ttcacgacga
    13741 gatcgtttgc ctgtctttcg agactgagat tggagggggt cacaccctgg aggaattgta
    13801 cctgatcgag ccccgaatgg gcatacactt gtagccccgt cctcaccacg tcctgcccgt
    13861 acccagggcc gaacacgaag gtattgtcgc cggtcccaat caacgtatca ttgcctgctt
    13921 ggccgtccaa ccggtgggag ccggacaact cattgtccaa agcattccca accaaattca
    13981 cctggaccgt agaatcgagg tacacatcat cctcgatcct cacattctcg aaattgtccg
    14041 gcgcctgaaa tgtaagcgat tcggtcggag tgaggtaaat cgtgtcccgg cctgcattca
    14101 cttcctcggt gatgacttgg cccatagagc ggagatgata tgcatcatct cctccaccac
    14161 caagcagctg gtctcccaaa cccaccgatt ccaaaatgtc attgccctcg ccaccgcgca
    14221 agacgttgaa tccttggttc ccatagagtc ggtcgtttcc ggcgccacca tctagttcat
    14281 catcaccttc atcaccttgc aagacatcat cgcccacacc accgagtaac acatcattgc
    14341 caccacgccc atacaataaa tccccaactt ggctgccttg catacgatcg tcaaaactcg
    14401 ttccttgtag cgtcccaccg gtttccggag aggacagctg aaaccctcgg gcgagcaact
    14461 cgccatccgt caacactgtg ccgtcagcaa attcaaactg tcggatagta tgaaactcgg
    14521 cgggatcgtt cagtccaaaa ttgctgaccc gaatcacatc ttcatcattg cccacgcgca
    14581 gaaccagcgt atctgctggt ccgacatcca aacgtagcga ttcggcagtg atgcctgcgc
    14641 caaagatgag tcgatttctt tgcacaccat catcttggat cgtatcggca ccatcaccca
    14701 ggttaaagcg gtacgtgtcg agtccgcttc cgccaatcag aaggtcgttt cccctacctc
    14761 cctggagatc atcctcgccg cctccgccct gaagcgtatc gtcgccctca cctccatcca
    14821 gcacatccga tcctgcttgt gtggagaaga acggggaatc tccgaatagg aaatcattgc
    14881 cggcttcacc atagagctga tcgtccccag catctccaac cagctgatct gatccctctc
    14941 cgccaaacaa ttggtcctgt cccgccccgg cccaaagctg gtcatcgccc tcttccccat
    15001 ccaacgtatc gtttccacct gcaagactca gaacagtagg attgttccca tccccgtata
    15061 gaaaatcact gccgtcaccg ccgaccaaaa aatcatctcc ttcacgtcct tgcacatcat
    15121 cgatgccatc gccaccgtcc agctggtcat tgccgagtcc gccattcagc aaatcatcgc
    15181 cggcttctcc tgaaagaaga tcatctccat cttgaccaaa cagcgcatca tgaccttccc
    15241 caccgaaaag agtatcgcct cccgcatctc cttgcagttc gtcgtcccct tcgtcaccaa
    15301 agagcgtatc gtctccctcc tgccctgtca cacggtcatt tccaagcccg ccccataaag
    15361 aatcgtcccc tgcgcctccc tgcaactcat cattatcagc gccgccatcg aggaaatcat
    15421 tcccgccaaa tccggcgagc cgatccatac cgattccacc atagagaatg tcgtcgcccc
    15481 cgcctccctg aagctcgtct gctcctgcgc caccgtcaag aatatccgct gacccagggt
    15541 cgccggagaa atcaccgact aagaggtcat ccccatcacc tccagagagc atgtccgctc
    15601 cggctcctcc gaagagcgaa tcgtttccct cacccccatc aagccaatca tcacctgcta
    15661 tcgttagagt gaaatctgac tcgccatata aacgatcgtc accggctccg ccgagcagaa
    15721 tgtcatcccc gccatcgccg accagcaagt cactgccggc ccctccgtct agatagtcgt
    15781 tgccgccaga caacgaaatg atccctgctt ggggggttcg attccaaaaa gagttgtcgt
    15841 acgccagtcc aatccaaccc gtaggacgat tttcgccacg aagcacatca tcgccgtcac
    15901 cgcccaacag cacatccgcc cccatctccc ccatcaacgt gtcgttaccc gcaccgccat
    15961 cggcatagtg cgccccaccg ccaatgatcg gttccgcaaa actcaaatag tttgctgttc
    16021 cgacagcata atcatctcca tcgccaccaa gaaacatatc cccatcggtc acgaaggcat
    16081 aatcgtcacc atatccgccg atcaaataat cgtccccgta tccgccaaag aacacgtcat
    16141 taccagcacc gccatccaac agatcatcga acgcgccaag gatcggcgca tggtctccgt
    16201 gaagaacatc attgccaccg ttggcataaa cctctatcgc ccctgctcca ggaacccact
    16261 cttccgcgtc cgcgtcgcct atataggtgt cagtaaaatg tcctgccgga actccggtgt
    16321 cgcgcggcat cgccgacacg tcccgcaact gaatccccat ctgaccgctt tggaaattct
    16381 cgttaacaat gagaaccccg ttgacgatca gatcggtccc tgacatgaca taggtagttt
    16441 ggccgtccag gcttgtatag gtgttagtgc catcgcctgc acggcggata ccaccctgca
    16501 gcaggcgggc gtcaaaaaca atctgcccct gcgcgtcgct gtcttcgatc cgatccattc
    16561 cgtcaccgga atgatagata taggtgtcga atcctatgcc gccttctaag aggtcgttat
    16621 cgccggtgcc gccaaggagg atgtcgtttc cgccgccccc tacaagatga tcctgccctc
    16681 cgagcccgct cagaatgttt attgcgctgt ttccaacgat cgtgttctcg agttcattgc
    16741 cggttccatt cctagcgctt gactcggtaa tcgtgaggtt ttcgacttgg gagtcgaggg
    16801 tgaacgtgac ggagcttttg acctcatccc tgccactatt cgcatactcc cgaaccacat
    16861 cgccgatgtt atcgactata tagatatcgt ctcccgtccc acccagcatc gtatcagcac
    16921 cgcttccacc atcaaggaga tcatcctctt gatttccttc cagataatct ttaccaccat
    16981 tcccatagag ctggtccttg ccttggccac cgtatagatg atctacaaaa aggagactcc
    17041 caataagtga atcgcctccc tgtccgccaa acagtacctg gggcaaggga agagcgtcag
    17101 aacccagctc ataccccaac tcaatatcat taaaatgtgt tagggatggg agactgatag
    17161 aatggctgtc cacctcgatc tttttcagca aaaacgccgc ccgatcctcg aaatacagcg
    17221 atgtaaggtt tcccgtaccg gtgttcggat ccaacagcga aagttcaccc gtctcattgt
    17281 ggcggctgta gagcgcttgc gttgtatctc gatccattcc tcgcaacacg aaggggttga
    17341 gttccttcag cgcatatcgg tacgcaagcc ctaaggcatc gttcggacca tcttgtaggg
    17401 caatcgtcct cattgtgctg gcagactggc caaccaaaga gtcaatccga taactgacac
    17461 cagtaagcgc ggcagtgact tgctgaatgt tttgatagaa ggcatccctg tttccgagat
    17521 tgccaaagtc gccaggttgc cgaccaaagg gggtacttga aacgtttcca agaaagactt
    17581 tgcgaagtgc atctaacgtt ttctccaggg tgtcaccttc ggctagtgga atgacacctg
    17641 gcaacactgt cacatcggct ttctgcgccg aaacagcatt gaaaatcccc tcaatttgtg
    17701 actgctcaag agtaggatca accttcagga atagctcttg aagggcaaag gaatccacaa
    17761 gaagagtgag actatgcgta ttcccatact gaaactctct ctgctgattg aacccctcaa
    17821 gcagaggttg tccctcaata aatatacttt gggaaggggc atggatccct gagttggcca
    17881 caaactgcac gtcactgccg gtctcagcat tcccgactaa ctgaacaacc ttatctaacg
    17941 cttcactctg cgtcgggata ccgcccacta ctccggcagg aattgtaaag aattgagtat
    18001 tccccgtgcc tattgttgga aatcgggtca gagtggcctg acgagcgaca tcatagcgag
    18061 catcgccata gatgtttcct gctgccccac tcctgaactg tgttccctct gggtcaaatg
    18121 cgagcagacg cccctcaaga tccaacatca tccgacgaag agtttgctcg accggctgcc
    18181 cgtcctgacc cagttcggac acgaaaccgc gtccagcgcc attgaaggtg taggtttgca
    18241 gaatgcgatc cgaatgcatc tcggtgaaga cggttgccag atggccgctt aaagaataac
    18301 cggtcacgtt gatcttggcg tcaggagcta atttgcctcc aacagcagtt agctcacgcc
    18361 agtaacgctc catactcacc agctgagcca aagcaaaacc atagtcctta atctcgccat
    18421 cggcccccgg caggccgtcc cgctgccaat ctccaccttg ggcctcatca cgatactcgg
    18481 tacttcggat ggaaagagta tattgttgcg tttctttatc gaatagaagc gtggcactaa
    18541 atccagtcgc gtcactggcg tgatggtcga caatgtcgta tcgagcaaga aatcgatctg
    18601 ctaatacgct tgtgaaccga gtttttccgg caagatcagc gtccgttgga ctgatcccaa
    18661 attggttatt gttgcccagt aataaacgtg gtttcaccaa gagttcattg tgcagatcaa
    18721 tatcttgaag ataggactcc gcagccattt gctggagagc aaaattgagc catgccgtcg
    18781 tgttacttga cattcattaa ctccctaaca ttcatctcat aattgatcgg cttaacaacc
    18841 tttccgataa accaataaga aaaatcaaaa tctctactgc gcccacctct gtgttgatag
    18901 tagggacaag cttgaccggt gagccaccac actcctgtga ggttgttacg aacgctccca
    18961 gttcgaatat atccacgcct tacccctaag acctctccgg tctccatgtc caatacaata
    19021 agttcgccac cagcaattcc catttctcga tcgtgaggac gaacgatacc tcgccatgta
    19081 tacccatatc tcgccttcct cactgaatcg aactccttga tcatggttag tggatctttc
    19141 ccattcgatc ctagatatcg ttcatgatac gcccgatcgc tcggggccgt gaaatacgaa
    19201 ttgtctagaa atgattttcc ccagctaggc ttcttccttg gggccagagg gtgttcaaag
    19261 aatttataac gaagcggccc aacgaaaaag aattccggat cctgagattc cgcatccata
    19321 tacccatatg gatcttccat ggcatacaga tgctcttgca tgtaatcatt cgcgtgctca
    19381 cgcggccgca tcatgtagag tccctcaacg ttctcagctg ttttgtaaat aaactctccg
    19441 gcttccgtct tacaaagatg ctcaaaatac tcttggctcg tcatccctgg cttatatccg
    19501 ctgtcttccg gcacaggatt cgggatcttg atcgaatgag caaaccgtcc ctcttcggtc
    19561 gccaacggat ctgctggctt gagcttggct acctctccac acacttcccc tcgggcgagc
    19621 ttccggcttt cacaactctg cttgattttc tcgatggctt tccggaaccg ctcccctact
    19681 gtctcatctt ttccgaacag ccccgcgccg gtgggggcaa tcaacgccag cgacaacagg
    19741 ccggcaatca cgaataggct taacattcgt ctgttttgct tcatgagaaa ccctcttcct
    19801 gaaggttcat gcaccattat ttgtgcatgg atttgcctac cgctcccgca aactctcttt
    19861 catcgacttc aataacgggc tcagcaaaaa ctcgatcacc cgccgctgtc cagtcttaat
    19921 ctccaccgtg acagccatgc cgggggtgag atgaacgtgc ttcccctcta cctgcatcgt
    19981 ggctcgatcc atactcaccc ggctcacata gaccagaccg cctttttctt tatctaacgg
    20041 taccgcatcg tcggagacgg tcaacacctt tccgggaatc gtcccgtaca acgtaaacgg
    20101 aaacgtttcg atcttgagtt ccaccggctg gccttccttc acaaatccaa tgtctttatt
    20161 ttcgagctgc gcttccactt cgaccgggtg gtcctgcggc acaaccatca gcagagtttg
    20221 agccggcgtc actactccgc ctactgtgtg cacggccaat tgctgaacaa caccatctat
    20281 gggagaaacc agactttgaa gttcagtttt ttgtccggct ttccgcacct cttgtacaag
    20341 tgacgtggct ttcgtctcca tggccgacag ttctgcttgc ttactttgct gaaattcaga
    20401 aatgagcacc tgatagttct gctctgcctc ggccaaggcc gcttgatcct gacgtagctt
    20461 gctcttttgc cctgcccatt cctgagcttt atcgatgcgt tgttgctcga attggagata
    20521 gtccatcttc gacacatact gttgtgccag caatgcccgg taagccgctg ccctttcatt
    20581 ttccatcgga acggtggctt cgagacgtcg gagattgtct ttggccgcat ctacagccgc
    20641 tttacgctgc ccgatgagat gtcgtgcagc atctacccgg gcggcatact cgaccatctg
    20701 atcccgtaaa agctgctgtt gcagcaacac aaattccggg tctgactcac tgggcgcggt
    20761 gaacgtactg tgcccggcta tcagcgcatg cagccgagcc gcctcgacca gagccgctcg
    20821 atattcattg gaagcccgat cacggtctgc gctgttttga gtcgcgtcaa gctcaatcag
    20881 aacttctcct cgtttgaccg attggccgtc ctgcacatga atcgcagcaa tcacacctgt
    20941 ctcgaacggt tgaatggttt tagaatagcc gctgggaata attttgcccg gcgccaccgc
    21001 cacgatatcg acctggctca acgaagacca tgtcacggct gaggcgaagg ccagcatgat
    21061 ggtccagaga atcgcccggc caatcggcga cggcggtgca tcctgaattt caagaattgc
    21121 gggcagaaat tcgatcgctt ctccttgggc tgcggcgtga gatgactgac cggaaccaac
    21181 gcgcccgatt gttcgccatc ctgccagctt gagttgaagg gcattcatca ttacgccgca
    21241 cgtccttgct gatgctgatg aagtcgcgta tacatcccgt cgcgcgagag caattcatca
    21301 tgcgtgccct gctcaaccaa ttgccctcga tcaatgacgt aaatcttgtg agcaggacga
    21361 accgtgctga gccgatgcgc gatgatgatg accgtacgcc ctttgcagat ctgggccata
    21421 ttgcgctgaa tgatcgcttc ggactcatag tccaaggcac tcgtcgcttc atcgaagatg
    21481 aggatgcgcg ggttcgccac caatgcgcgt gcgatcgcaa tgcgctgccg ctgtccgccg
    21541 gagagcgcgc agccatgctc cccgacgacc gtgtcgtatc catccgtcaa ctcaagaatg
    21601 aactcatgag caccagccag tttggcagca tgcatgacac ggtccatggg aagcccagga
    21661 tcggtcaacg cgatgttatc ccgcactgac cggttgaaga gaaagttctc ttgcagcacc
    21721 acaccgactt gacgccgcaa ccaggcaggg tcgatctgcg cgagatcaac accgtccacc
    21781 agaacccggc cacgctctgg cacatagagg cgttgcatca gcttggcaat cgtgcttttt
    21841 ccagatcctg agcgccccac cacaccgacg acctgccctg gatgcagagt aagagaaaca
    21901 cgctgcagca ccaacggccc atcgacacga taccggaacg ccacatcgtc aaatacaatc
    21961 tgcccggcaa tctgcggaag agtcgtgcgg ttgggattgt aggacggttc tagcttggca
    22021 ttgaggacat cccccagtcg ttgtatcgag atgccgactt gttgaaattc ctgccagagg
    22081 ttcaccatac gcagtaatgg gccggttact tgtcctgaga gcatgttgaa ggcgatcaac
    22141 tctccgatgc ttaaggcgcc gtcgatgacc tgataagcgc ccacccacat gatggcaatg
    22201 gtcgtagatt tctgaatgca agtcgctatt tgcccagcca cagtaatgag actagtggcc
    22261 cggaaactgg cggcgacgta tccggctaat tgctcatccc atcgacgctg gagcggtggt
    22321 tcgacggcga gcgccttcac ggtttgaata ccgctcaccg cttccaccag gaaggattgg
    22381 ttctccgcgc cgcgattgaa tttttcatgg agtcgcgcgc gaatcgctgg cgtaatgagc
    22441 acggagagca acgcgtataa gggcagcgag gccatcacca ccaatgtgag tgtggggcta
    22501 tagatccaca tcacgcccaa aaacacgatg gtaaacacca catccaggac taccgtcacc
    22561 gagttactgg tgataaattg gcggatctgc tcgagttcgc gtacccgcgc caccgtatcg
    22621 ccgacccgtc gggcttcaaa gtaggctagc ggcagcgcga gaagatggcg aaacagttgc
    22681 gctcccagcc ccacatcaat ccggttggtc gtatgggcaa acaggtacgt acgtagccct
    22741 ccaagcagcg catcgaagag ggcgagcgcc aacatgccaa ccgccataac atgaagtgtc
    22801 gtgaatcctt tatggacgag cactttgtcg atgaccactt gcgtaaagag cggggtcagc
    22861 aacgcgaaca cttggaggaa gaacgaagcc agtaccaatt cacccaacag acgtcgatac
    22921 ttgaggacgg ctggaataaa ccaagtcatg tcgaagctga catcttctac tcgcaagcct
    22981 gaccgtttgg taaaaagcag gaggctaccg ctccagcacg cgacaaactc ctccttggaa
    23041 agaatatgcg gccgctcttt cagaggatcc tgaaggaggg ccttttctcc ctgtactttg
    23101 gccaggacga cgtaggtgcc gtcagttcgc tgagcaatcg ccggaagtgg cgcccccgcc
    23161 agttcacccc acgaactgct caccaaaccg gccttgaggc cgacgtgctt agccgcacgc
    23221 accaaggcgt ttgtcgtcaa catatggcca gatagtccaa attgatgctg aatctggccg
    23281 ctatcgatag gcagatcgtg aaaccgcgca attaacagca ggcacaacag tccggtatcc
    23341 ggccgaggtg gcgccgttga acaagactcg agatgggaag ttgggacatc catgacggga
    23401 tagcgattgg cgcgttagga cagttttgat ttggcgggct gcaggctgac gaccatctgc
    23461 tgaagttgtt ggtgcaggcg gatcaccgcg tcgagcggca atgccactct aacggccctg
    23521 cttcccacaa gctgtttcgg catcgcttct ccattttggg cacgttgcgc gaccgcaccg
    23581 atcagactcg gctcgagaaa tccaaaatca atgtaggcca atccttgtgc gagacttgca
    23641 tgtaaatagt tcgccagaat cggctggtcg gcctctgtac tcgattgaag acgaacgttc
    23701 accgccactg ctttatcacc attctgcttg ggactgcttc caacggccat gcgcttctcc
    23761 tttaagcaaa tgaacgacat actggaacga ataccccgca tccgcacgat ggcgatatta
    23821 gtcgaaggtc cctgcgtgga atattccccg gacgatgggc gatgagaaaa actttggtag
    23881 cccagagatg tacttaggtc tttgaggtcc tttaatgcga ggtggaccta gtggcaatac
    23941 ccaagagtgc cacagctgga cttagtccaa tctatttcta catggaaatg cagggacata
    24001 tgacgtgcgg cgccttgcag gtcgagaagg ccgatccgtc tatcaattct tccctctcct
    24061 acgtcgtcag tgtagactga acaatattta cttcttgcgg aggaacgatg cagaaagttc
    24121 tcatgcttca ggccggatgg cgcacgctgt gtcctgtcac ggcactatgc ttaagcctgt
    24181 ccgcttgtgc gggagtgagc acaaccgaca cattgccgac cgtggcatcc gtcgatctcg
    24241 ctcgatatgc cgggacctgg catgagatcg cgcgcttgcc gatgtggttt cagcggcatt
    24301 gcgtggattc gaaagccacc tacaccgtgc gcaacgatgg cgctatcggc gtgcgcaatg
    24361 aatgcgtcac tgataccggt gggcatgatg aagtcgaagg ggtcgcaaca gtggttgatc
    24421 caggcacgaa tacccggctc cgcgtggtgt tcgacaattt cttcgcgcgg ctgttcggtt
    24481 catcccgcga aggcaactat tggattctgg cgctggatgc tgattaccag accgccatgg
    24541 tagggactcc ggatcgccga tatctgtgga ttttgtcgcg ctcgcgccaa ctcgacgaga
    24601 ctgcttacac ccgcctggtt gagcaggcca gacgattgag atttcccgtc tcagagctta
    24661 ttcgtcccca ccgatctgcg gcaacatcat agaaacggta gctacgtctc atctcgtctc
    24721 gtctgatcgc tagcctctca tatcactgcg gccctctgct ctctacggtc tctgccacga
    24781 acctcagccc tcagtttcgg actcgccttg cgacgcaaca tgaaccacga cggtctcctc
    24841 cgttggtttc acttgcgagc cgtggtgcgt ggcgtacacc gcaatgaagg ccgcaccaaa
    24901 cagaaatact tgggaggcat agtaggccca cagcaacact aatactaagg aaccggccgc
    24961 accatagaag gccaccagtg tgttatgcgc taggtagacg accagcagtt tgcttccgag
    25021 caagtagagt attgccgtga tccctgcacc cacccacaca tccggccacg ccacctgagc
    25081 ctgggggacc cacttgtaga tcatggcaaa cagcgtcgag acaacgccga atgacatcaa
    25141 gagcccaata ccctgaacca gtcttcccgg cattcccgtt gtttccaata cgaactgaat
    25201 ggcgatgccg gtccaggcgc tgagtgtgag ggaagccagc aggatgaagg caatgccgaa
    25261 cagcccggat agtgatgcaa gtcggcgttt caggcggtct cccatccccg gaggatgttt
    25321 gggttgaagt ccccagatac aatctaatgc atccctcagc tgatccatga ctttcagcgc
    25381 gccgatgaca aagaccccca gcgcgacgac tccgttcacg aggggggacc ctgccgatct
    25441 ccactgagca atgatgtgaa agagtccatt cgctgccgat tctccgatga gttccccgat
    25501 ctgccgctga aggccgccaa ccattgactc gtcgcccacc gtgctcacgg agaccatgat
    25561 cgtcagcacg accaagggca cgatggtcaa tgccgtataa aaggcgagtg cggccgcgaa
    25621 tcgcggtcct ggctcatcgc tccatttcgc aatggtttgt gtcgcgagag acttgagcag
    25681 ggcccacata gactcacgcc ctcctctgcc gtggaaacct catatcccca cgttgggcac
    25741 gatgcacatc agcctgtggc cgttgatagg acactcagtg catggaagcg gtggtggcat
    25801 ctgactcagg tcagaccggc agcgaagatg gggttgccac cgccgttcag cctgtcggtt
    25861 tgtcatctgg cctgtgtggc gtggtgggtc gttgcagagc ttcagtggca atggcttcga
    25921 tcttacaaca ggcatcatag gcatggtcat gtcccagcat cgcgccattg cgtgccaagt
    25981 caatgatgcg ctgaagggcg tcggcatatc cctgcatctc ggaattcaag ttggcgcggg
    26041 cagcagacgc aaggttctgg gccttgtcca tgagaagcct ctcactccac agagaagcca
    26101 ggccaagtgg atgagacggg gacactcaat ggctacacac gaataattct cggagccacg
    26161 acagaccttt gaaagacaca cccaatgtcg aacgttgtga ggcggcgccg ttccgaaacg
    26221 tcactcagag actactcaga gacttccgcc ctatctataa aataagaggt ttccccgaaa
    26281 cacgtggtgg ggacgtagcg gaactcccgg taatgtagta caacccgttt tcccatttgc
    26341 ccatcaagct tcttcgcgac atcatcgtcc catacgctaa agttccacag aacgggagcc
    26401 acgccaggaa ccgtcgtcat cgccaactcg ccttcatacg ttttacagac ccaccctttc
    26461 cgagacagtt tctgtaagta ccctgtgcgg tctccgtccg aataattaca gccgcccagg
    26521 accgctatgc acaacaccag ggagacgtat gtcggtacct tcatcatgga tgatgcatta
    26581 gagcattttc gaggcgggac gatacctagg cgtccccctc gtcgaccagt ggaaagactc
    26641 gccggacaag ctcaggaaca tcccgccgcg ccagtggcgc ggagaaaaat agaagagaat
    26701 aaagttagcc gaacgtcacc gggcggagca gttgtgcggt aggatgagtg aggacctatc
    26761 atgagaagga gacctatgat tgccacactc gcgaccgcac tgcttgtcgt tctggccacg
    26821 gttcccaacg gacatgccgt ggctcaatcc agccagcgag atgtctttga tgaacgcgac
    26881 acccgtgtat ttaaggcagt ggggtgcaca caagataagc aaccagccga agcgctgtac
    26941 tatatcgtcg cgagtcggtc agatctatcg gcaggcgttc cgtctccgtc gtcgcaattc
    27001 atgaaagacg aagttgaaca gaattggcaa cggatcgcct cacagttgac gatggagcaa
    27061 gtgaccgaag agcgttttac cgatacgtat cacgcgctgt tgaatgacat gattccccgt
    27121 ttacagcggg ccgtggaaga gaagagtggt gtatccattg cggtctcgga ggtgaatagc
    27181 aggccgatgg atcgtacaaa agaccaggac gtcccgacct gcagttcaca gtaacggcag
    27241 tccaccaaac cacccaacca tcgcctcccg atcccatcgt ggccgaagag caggcaactc
    27301 ggtactgctc atctacgcac ttcgtgccga gatccacgta ataaaacgcc gctccgctcc
    27361 actgtggcct ctccagccgc cgattcagac cggcttgagc atcgcttgcg gccccagcct
    27421 ctccgacctg gggttattcc ttgggtcctc gttgtcctct tcagtgctaa cgtgaatgag
    27481 taaccttaca cgaggtgcca tatgagtacg atcttattga tcatccttat tctcttgctg
    27541 ataggcgcgc ttccgtcctg gccgtatagt agcggatggg gctattaccc ctccggcggg
    27601 atcggtctca tcctgctcat cgtgctcatt ttggcattaa ccggaagagt ctagacgcgc
    27661 cccctttgac ggatcctttc gcgaggacgc gcggcgttca acgacgaact tgtatcctta
    27721 aagaagaacg gccgtctgga tgaggcagta tcttttatcg tcactgcacg gatggccgat
    27781 caagttcatc ggataataag gacgggagtt ccaccgccag cggatcttcc agttcgatcg
    27841 gttcgtaatg gacggctaca atggaagtga gcggacagga aagccatcca ctcatgccgc
    27901 gcgttttgaa acgaatgccc atcggcggaa gggcctcaag atcgacgctg gcaatggtga
    27961 taccctcgcg gtcagtccat tgaaatgtat ccatcgtcag tgatccaggt atcacctcat
    28021 accaggaacc atctcggaga aggacgcgag cgactgtcgt gacatcaatc ttcaggctca
    28081 tggctcctcc ctcgtcgtcg cccccttgac ggcctctttc ttgccccagg agacgttcgg
    28141 ggtgtggcgt cacctcgatc gccttgtcct ttgttaacca ccgttgtggc ctttcgccgt
    28201 cgatcctggc tcaatcaact cgcgcctggc agtgtggtgt ggcataaggg ccgcccttta
    28261 ttggcagggt aaagcctaat gacgctgatg catgctaggt attcccctat tggatccaga
    28321 agacacaaag gaaggcaggt agacggtcat ctcaaaaccc acaagaacga tcgcaacaaa
    28381 aaagcgcaca gctcctctag atcgaggaac ctgtgcgctt accccaagca gggcttcccg
    28441 ttatcgctca agaggccgcg tgttcgcgca acccgctctg gttctcgatg ttattcgcta
    28501 cctgcagaaa ccccgttaca cacgaaggtc ttcctacttt agacgcttcc ggggagtgcc
    28561 cggcttatcg acggccttcc ctcagcacgt cttccaaatg ctgccgcaac gtaccgagct
    28621 ctttggattt aaacgagctt ttcgtgtgct ccatgacttg gatagcctct tcaattgccg
    28681 cttcgtaccg gtccaatcga gggcatttca tcactgcgca gatatcggtc ggggcctgtg
    28741 gttgacccgt tcgagacgcc tccagagtgc ggccgaccag cgccgataac tggctgatat
    28801 gccccatcgc ctgagccata tactcctgcg ccgtccattc cacctgctgc cccctccctc
    28861 cgaactcgtt catcgaacgt cccaggcgct caaaaatccc agccatcttt gcggtttcgt
    28921 gcatggcatc ctgtacggcc cgcgcaatgc tccttcgacg gaggcctcga tctacggctt
    28981 tcttgagctc attcaaatca accggtttga tcaaatagtc cacgatcgac agtcgaaatg
    29041 attcaatcac tgttcccacg gacgggtatc ccgtcaccac caccacagga aggtcaggat
    29101 gtcgcctggc gacagtttca agaaattcca gttgcgagtt tcccggcatc cggacatcnn
    29161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg gacctcgatc ctgccaccca
    29281 tggctcggac aagcccgcgg gacacagaga gtcccagtcc gagcccctgc ggaccgccgg
    29341 ccgatttcgt cgtaaaaaat ggttcaaaaa tatgcggaaa tacctcgggg gtaatgcccg
    29401 gcccatgatc catcacgctc caatggagcg tgtcaccgtg ccgggccacc ttcaacacca
    29461 cttccatatc ctggggagac gcatcgatgg cgttctgcac gagattgagc aatacctgaa
    29521 acagatcggc gcgcggcagg gtcaccaccg gacaggtcaa ctctatttct gtgcgtaact
    29581 ggattcggcg cgagaccagc ttgtagtcca gcaacgttcc cacattttgg agcaggtcgc
    29641 tcacgtccgt ggcttgccat tgtcccgatg ctccattctg atagagcgtg tacattttct
    29701 tcacaatggc cgcgaggcgc tggatctctc gatcgatgag ttcaacgaat cgataatgcc
    29761 ggtggtcggg gctcacgcct tgcttgacga ggtgaaacgc gttcttaata cccgccagtg
    29821 gattgttgat ttcatgggcg atatcggccg ccagatggcc aagcgccgcc actttttcgg
    29881 cctgcgaccg ttgagcctcc aattcatgaa ttcttgcggt tcgctcacgc acacgttgct
    29941 caagctgagt ttgcacttcc accaaagcct gctctgctcg acgacgttcc gtgatgtcat
    30001 ggaatgaggc tacgacggca tacggacgtt cggcatgagg ctcccgaaga ggctgcgtat
    30061 taatcgacaa ccatcgttgt tctccgcccg gtcggcatac acccatgatc acgtccgcgc
    30121 agggctgccc cgtatggagc gtgaccatgg caggatgctg ctccccctca aacggcgtgc
    30181 catctttgtg aatagcccgc cactcagaag tgacggatgt tctccccatc atctgttctg
    30241 cagtcaggcc cagaatctgt tccgcactgg ggttacacgc aatgatccgt ccttgcgcat
    30301 tttgcagcac gatgccttcc gccaacgccg tgacgacgcc ccgatatcgc tcagccgttt
    30361 caaccaacgc cgttttggtg cgtcgcgctt cggtgacatc ttcggccaca taggaatacc
    30421 ggtgccgctc gctcggccct gtaccgatcc aacagaccga gacggacaaa taccgctctg
    30481 tgcccgaggt tggatctgaa tggggatatt caaatcgcac cgattgtgcg ctctctcggc
    30541 tcatctcgta gtgtcgatgc cacaatgcga tggtgtccgg cggcgccccg agcttcgagg
    30601 ccaactgccc ctgcatcaac tccgaagtag ttccaaaaaa gctggccaca caacgattgc
    30661 ctgagaggtg cagaatatcg ccgtcgtgta attccactac tcccatcatc atgacgccgc
    30721 tctcgaagaa gctccgaagc gtggactcgc ttcgagacaa ggcctcctcc gctcgcttac
    30781 gttcggtgat atccacatgg gaaccagtca tacgccgagg acgcccctct tcgtcccgtt
    30841 gaaggacggc gcaggtgtgg atccatcgat aggttccgtc tttatgccgc aaccgatgct
    30901 ggagttcata cgaggcgatc cgccctgaaa ggtaatcatc caccagggcg aaggcacgcg
    30961 ccttatcgtc gggatgcaac cgggtttccc actccgacgg cgagtttgaa atctcctgct
    31021 cctcatatcc caaactcgac ttccatcggg gggagtaata catcgtgttg gcctgcatgt
    31081 cccagtccca aattccggtc tgcgtcgcgg ccaccaccag atctaaccgt tcctgagcct
    31141 catgccgcgc cacttcagcc tgcaagcggg ccgtcatatc cgtcgccaca ccgccgaccg
    31201 ctatgatccg ccccccttcg tcacgaagcg gaaacttacg cactacatag aagcgaggat
    31261 cgttattcag cagggcgtct tcttcgaaac tcatcacctc gccggtatgg aggaccttgc
    31321 aatcatgttg tcgataacta tccgcgatat cttggggaaa cagttcatga tccgtttttc
    31381 caatcacctg c
//
LOCUS       DAOQEW010000019        31236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_312_length_31236_cov_7.196370, whole genome shotgun sequence.
ACCESSION   DAOQEW010000019 DAOQEW010000000
VERSION     DAOQEW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 31236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_312_length_31236_cov_7.196370"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLS35_03720"
     CDS             <1..302
                     /locus_tag="PLS35_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HNI17794.1"
                     /translation="FVLAGLFEIGGGYLVWQWWRNGSHWSVGLLGAVILILYGIVPTY
                     QPSHFGRVYAAYGGWFIILSILWGWLVDHVEPDRFDIIGAMVCLVGVTVIMYWPR"
     gene            670..1566
                     /locus_tag="PLS35_03725"
     CDS             670..1566
                     /locus_tag="PLS35_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17795.1"
                     /translation="MANQDETLKHIWKSGFPFQLKIEDEVRRTHSTHNWLIATREHPW
                     TNKETESSGFIDLVLMHDHFVTYRMVIECKRVKADDRRQLHWLFLIPEGEKQETPLAS
                     CLQIGGTSPMNARIGEANVSQQEWDHVRIWENVNIFPKSFESSICVLPSDDSKSQPIL
                     ERLAAGVIDSVEGLGDEEILVHQSQFQVDRLIAFLYPVIVTNAEIAICSFDPSKVSIL
                     DGTLDASAATITTVPFIRFRKSIATTFPEGTFSNLKEANEARELTVFVVNAGALPSLL
                     TNWRMASRDLSGRYAIQNHLQR"
     gene            1612..2334
                     /locus_tag="PLS35_03730"
     CDS             1612..2334
                     /locus_tag="PLS35_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNI17796.1"
                     /translation="MPTHDTFLSYASEDNDFAFQIAYGLRANGISVWFAPLMLKVGDR
                     LLDSVELGMAESRTGTLLLSPTYLHKGWTSYEMDILIRQHIEGTKPLLPIWHNASKKQ
                     VDAKHSGLGGIVAITDTSSATEVVFKLIEGLSSGAPSRAVVPIWEDPTYRFLEGLAEV
                     NLQSGEGAATTIFEFLLHAKESQYPLWIGGRVFTKNQLLLHVAQLLGPVPDRVKSWVH
                     EDGYRKLWEMCVENDLNPHNFY"
     gene            complement(2657..3148)
                     /locus_tag="PLS35_03735"
     CDS             complement(2657..3148)
                     /locus_tag="PLS35_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17797.1"
                     /translation="MPDRFHPDEALKNEAISQAMEGDFIGARQTGAKIAARQDFLEAW
                     MYILHLQRDRGDVIGIKETILSCPNPSWLQGHSYAELARHFAKIGNKTAAIEIAKLLG
                     RSGEMMVVYLSAPVDLILKGDFAGAREAVAKVEDEQLRNQLNALVDREEQIRSGPTQQ
                     SVL"
     gene            3519..4115
                     /locus_tag="PLS35_03740"
     CDS             3519..4115
                     /locus_tag="PLS35_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HNI17798.1"
                     /translation="MKTAWMALLALCAVAQTGEAALIGTDVMFQSVFQATSTSPEFKL
                     TSLNTVTVKEPGVEFPSLKALQVSNPSGLRLVDIAINIGDTFIDMNFTNSAPDFRFAS
                     GFRNGYKLTFDNAAMPSIRQALIDRTVTTLGLSDSDVTFSGNTLQVNVESLPFNTSTF
                     ARINLTVEGGPSPVPVPAAAWLFGSGLAALVALKRSGA"
     gene            complement(4517..5401)
                     /locus_tag="PLS35_03745"
     CDS             complement(4517..5401)
                     /locus_tag="PLS35_03745"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNI17799.1"
                     /translation="MKDNKTAVLRKLTEDSAMQLLEGVTLPPQPGTLLDINRERAKAE
                     PDVAAVAGIIERDLSVAAAVLKTINAPWFELPKKVESVRQAVVLLGMPNVVNVVIALA
                     MKSELQGNNQAGIDRFFDEANDTAMVLSSVMREVPVMSTDKAYLLGLFRDCGMPILLQ
                     RLPQYAEVLQEAAKRPEQSLPDIEDARLKTDHAVVGALMSRHWHLPEEIGTIILNHHR
                     TRELFGGKAQKLASSEESIRTVIAVLAMVDCICHRLRKRNDSPEWAAIGEKVLAHLKL
                     DQAEFEHLAWDQQDRFAA"
     gene            5795..6253
                     /locus_tag="PLS35_03750"
     CDS             5795..6253
                     /locus_tag="PLS35_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17800.1"
                     /translation="MRTTVLSSELAHSTSRIGFMRSVFELLGIVLVRFRPVQRMWGAW
                     LIAVNAACLLFITHIEAQVALAAVGLALLAQALIYQRTRFIRLLGVTHLIWVPMLAWM
                     AVRLAAVPEEDVAFQAWLLALIATNAISLAIDAWDATRFVRGERQPHYVW"
     gene            complement(6880..7278)
                     /locus_tag="PLS35_03755"
     CDS             complement(6880..7278)
                     /locus_tag="PLS35_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17801.1"
                     /translation="MQGFTPAFAYFLIVFGAGFLLGTIRVLLLLPVLGERAAELLEMP
                     FMLIVIVLAARWIDRRFLAGVDDRGRIVVGLLAFGLVLSAEFVVGIALRGMTPRDVLF
                     ARDPVSGAAYYLSLLLFAVMPWLIAPRRRR"
     gene            7645..8019
                     /locus_tag="PLS35_03760"
     CDS             7645..8019
                     /locus_tag="PLS35_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNI17802.1"
                     /translation="MAQSKQVAALLGPSLIVVSVTELLNLEIFTNTSAHLTYLNGTLP
                     VVAGLTIVRAHNYWMPRWPVRITLTGWLLLLGGLIRMVAPVSSQQTAHHPTAVLAMTA
                     VLLVCGVVLTVNGYRQRDVRDS"
     gene            8098..8514
                     /locus_tag="PLS35_03765"
     CDS             8098..8514
                     /locus_tag="PLS35_03765"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI17803.1"
                     /translation="MDIQYKNDTEGVDWERMSELFERVGWGKRKAEDIQAAFRSSSYV
                     RCAYDERTLVGVGRTVDDGQYYALIVDLVVDPQYQGRGIGSNILSYLEHSLANYVFTT
                     LTAAVGKEGFYLNQGWRRHTTSFIWPRTERQRKDHT"
     gene            8851..9159
                     /locus_tag="PLS35_03770"
     CDS             8851..9159
                     /locus_tag="PLS35_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HNI17804.1"
                     /translation="MLELRPTCEHCNTPLPPDSPEARICSYECTFCAACVTDVLHNVC
                     PNCGGGFEPRPIRPSKNWRGENYLGKDPASTTIRHRPVDPSAHRAFAATLNAVPPEQR
                     "
     gene            9588..10406
                     /locus_tag="PLS35_03775"
     CDS             9588..10406
                     /locus_tag="PLS35_03775"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI17805.1"
                     /translation="MVSVPLTNLKNDSINSDDAILLFENGGHKVYWIGTHTGGDEIEC
                     NTYLIIDGHEGYILEPGGFDRFPHVFKKVCLLLSPNSITHLFFSHQDPDICASFPSWT
                     KWNDDIKLVVSSLWTRFMLHYESRDLSGKAHNLNFVKVPDEGLSIPLNDGGRLDCLSA
                     PFLHSPGNILVHDTVSGFLFSGDVGAAMYKDHTFRLVIDDWNAHTQAMQGFHQRYMSS
                     NRAVAGMNKQLAPLPITAILPQHGCIFRGDEVSRFTQWLGKLPVGADYFYPDLA"
     gene            10697..11941
                     /locus_tag="PLS35_03780"
     CDS             10697..11941
                     /locus_tag="PLS35_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNI17806.1"
                     /translation="MDDNALENTAIVTSQTGVVARWQQIPLYGRIIIGVILGLAAGLA
                     LGNQAAFLATPGKLVLRLLGALAPPLILVAIVHTFMTTHLGGPLAARLPRLLLMNTLV
                     AITVGLTVANVIQPGRGAGLAPAEPAVETTHTANPLVAFIENVPKSLLGPLGDDGKVI
                     GVIFVAVAFGMALRKERHRPLGTVEHLVELVLESLIKILHWIIAVVPLAVFGIVASIV
                     GTEGFAQFQALGIFVLSVLLALTIQAAYYLVRVRFGSWVRPGELLRGGRDALVMAFST
                     ASSTATMPVTYAALKDRIGLREQSASMGALVGANFNNDGTALYEAMAALFIAQMIGID
                     LSMHQQVMIVLTSIIASVGAAGIPEAGLVTMTMVFTAVGLPVQFIPVLLTVDWFLDRC
                     RTAINVMGDMNVSCLLDGKQKG"
     gene            complement(11966..13024)
                     /locus_tag="PLS35_03785"
     CDS             complement(11966..13024)
                     /locus_tag="PLS35_03785"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI17807.1"
                     /translation="MMAFSLKPLRQQIIVITGATSGIGLTTARMAARRGARLVLAGRT
                     EGALTELERELSQGQHRAVTVTADVGKAADVQRISRVARQMFGGFDTWINNAGVSMYG
                     RLLDEPIEDMRRLFETNFWGLVYGSLEAARYFKAHDQAGAIINVGSTLSDRALVLQGM
                     YSASKHAVKAFTDALRMEVEMDGIPISVTLIQPGAIATPYSQHAKNYMKTEPTNPPPV
                     YSPETAAQTILYCAENRERHVYVGASGKGIGMMGYYAPRLADLLMETLFARQQQSDRP
                     PARPRRDNALDRPSGDLRERGDYQGHVSESSLYTTASLHPMLTGAALVGAGLALKTLW
                     PSKHTSERVQNAPRAQRR"
     gene            13460..14464
                     /locus_tag="PLS35_03790"
     CDS             13460..14464
                     /locus_tag="PLS35_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HNI17808.1"
                     /translation="MWRSRIATAALAVALISPLSVLAEEPPANPKSLAEQLVDAFNGV
                     FGVHPGMRANHPKGMVLEGTFTPTASAASVSKAAHLQKKKTAVPVTVRFSAGSGQPSV
                     PDTSQMPRGMAVKFSLPDGSKTDLVVLSFNGFPVATAEEFRDFLLAVGASGPDAPKPT
                     PIEQFLATHPAAKHFVESPKPQPVSYATLPYFGINAFKFTNAKGHVTFGRYQLRPMAG
                     EHFLTQEQLNTMDPDYLSAEIRERLRRGPAKFTLLLQVAEKGDKIDDPSIAWPDSRKQ
                     IKLGTISITKAVADSQAAEKKLLFMPGALVPGIEAADPMIAARSASYVVSLSRRAQAQ
                     "
     gene            14544..14954
                     /locus_tag="PLS35_03795"
     CDS             14544..14954
                     /locus_tag="PLS35_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17809.1"
                     /translation="MARWSRVWLGPEGLWLMISLAVYVAAAMNQPSTPAGNDFLETLW
                     VAIPLVGIPLTFATALLPRGSGWWWLTRVVVASCIGVIVASFIAAGGVDYHDSRNSGL
                     LGAPVYSLSIGLLLLFPLTILAILLIWKKRRTSR"
     assembly_gap    15250..15349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15350..16636
                     /locus_tag="PLS35_03800"
     CDS             <15350..16636
                     /locus_tag="PLS35_03800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI17810.1"
                     /translation="STGLLGELLAQYHPRNFDIRLWDGTIWPADPGATPEFTLILRTP
                     TVLRDMLWKASELALAEAFISGDLEIDGDLEQAIPLAEYLSRHPVTWGSWLRLGFKSL
                     RWPSNPSFTTNGLAAHVTGPNHSLLRDKQAIAYHYDHPPEFYAGWLDPQMVYSCAYFS
                     FTDDLDAAQVHKLDYLCRKLRLRRGERLLDVGCGWGALIVHAARYYGVQACGITLSEK
                     QAEFAEACIQQHGLMDRCQVELRDYRQLSTPNRYDKIVSVGMVEHVGATHLSGYFRHI
                     WNLLKPGGTFLNHGIGIRHGERCAFGPFVNTYVFPDAELTTISTTTGFAETAGFEVRD
                     VECLREHYVLTLRQWRQRLEKNVTPGSSSMDESTWRLWRLYLASAAHGFATGKLNLYQ
                     TLLSKTQEGSAGLPLTRDDWYEAHSSIDSRLSGRLR"
     gene            16805..17176
                     /locus_tag="PLS35_03805"
     CDS             16805..17176
                     /locus_tag="PLS35_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17811.1"
                     /translation="MKRDTWLVVTAVTGGIVVWLLVSAFAHKREAWDSDLYFTLGLPA
                     VCLLSALLGYLEPNRAWRWGAAPLVGQALVMLLTQGPGTVLPLGIMMFGVLALPSIVT
                     AKMGAYAAKRSLHRSTARQNH"
     gene            complement(17201..18082)
                     /locus_tag="PLS35_03810"
     CDS             complement(17201..18082)
                     /locus_tag="PLS35_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNI17812.1"
                     /translation="MRIATWNVNSLKARLEKVLWWLDRAKPDVLLMQETKLSDADAPI
                     DAFAQAGYALAHHGEGRWNGVAIASRCGISGVISNFGEPLQPARSTDTGDDEPFAEAR
                     MLAADCGGLLIICLYAPNGREVDSPFYEAKLVWFDKLARWLKQTGAPQKACVIGGDFN
                     VAPQDIDVWDPAACHGGTHVSDRERRAFDRIVREGFVDAYRCHHPEPGRYTWWDYRAG
                     NFHKNIGMRIDHLLVSTPLRDRLIWSEIDREARKGKPLPSDHAPLVIDLDTAGHPFDA
                     GWVSAESRIAARTGKKR"
     gene            complement(18150..19802)
                     /locus_tag="PLS35_03815"
     CDS             complement(18150..19802)
                     /locus_tag="PLS35_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNI17813.1"
                     /translation="MTLLQPNTPWWQSAIVYQIYPWSFQDSNGDGIGDLPGIISRLDY
                     LNGSPDSLGVDAIWLSPIYPSPMHDFGYDVSDYCDIDPRFGTLADFDRLVSEAHRRGI
                     RVVMDLVLNHTSNQHPWFIESRSSRTSAKRDWYYWADGKSGRRPPNNWAARFGGSAWT
                     HDPHTGQYYLHSFLSEQPDLNWTNPAVRDAVFGVVRFWLDRGVDGFRLDAINWLGKDT
                     SWPDNPRRLAWRSYYRQIHRYDRDQPHTHEALRALRSSLKDREDILLVGETSSDTPGG
                     PAAFYGNGSNELHEVFDFRLLRSDWHPDTFRRLILDSDRAVPQGGWPPVVFSNHDQSR
                     HIDRYGAGGEAIRRARAAALLLFTLRGTPFVYYGEELGLHDGRLRRADLHDPYTIRYW
                     PWKTGRDPARTPMPWDDSPQAGFTTGRPWLPLSHAWRQTNVAGEQRDPASILSLYKRL
                     IRMKKGSNALTVGTYHPIDGVPPECLFFTRESLVDGQREALFIAVNFSAHTQKISLPA
                     GPSNLERHGTLMLSTDPQRAEGPWTADHFLLGPDEGIVVSMQ"
     gene            complement(20117..>20362)
                     /locus_tag="PLS35_03820"
     CDS             complement(20117..>20362)
                     /locus_tag="PLS35_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17814.1"
                     /translation="MHVLTNKKGAIVGGGLLTPGKDHKGKPVHIQIEPMKGQSLKEVA
                     VPDELARLEGADFFSRLMCDFHLPRGKKELVRRGSKR"
     assembly_gap    20363..20462
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20621..21049
                     /locus_tag="PLS35_03825"
     CDS             20621..21049
                     /locus_tag="PLS35_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HNI17815.1"
                     /translation="MTTIDPTRHEWARFERLIVFDGICNWCNAWVNFTMARDRGRFHF
                     ATLQSAKGQELLTLLGLPVQDFETFLLLERGQVFTKSTAALRIARTLSGWWPLLYGFI
                     LIPRGLRDILYNYVARHRYRLMGKADTCRIPSTTDRDRFV"
     gene            complement(21046..21270)
                     /locus_tag="PLS35_03830"
     CDS             complement(21046..21270)
                     /locus_tag="PLS35_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HNI17816.1"
                     /translation="MDQNFKVGDHVSWNSEAGRIRGTIQKRITAPMKFKSYIARASKG
                     QPQYLVKSDKTGHLAMHKGTALRKIGGQSA"
     gene            21411..21929
                     /locus_tag="PLS35_03835"
     CDS             21411..21929
                     /locus_tag="PLS35_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17817.1"
                     /translation="MDIVSHGLWGALAFGRRSRTSFWLAFGIGLAPDLFSFGILWAAA
                     TLGLSPKPDFSHGTPPESTIPPYVHHLYDVTHSFVVFLAVFALLWLVMKRPVWELGAW
                     GLHVLVDIPTHSFAFFPTPIFWPLSDWKFDGWQWSQPAILIPNFTVLLLLYGWFLFQQ
                     ARPILRPERARD"
     gene            22042..22422
                     /locus_tag="PLS35_03840"
     CDS             22042..22422
                     /locus_tag="PLS35_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17818.1"
                     /translation="MANRKREPVPTPQSIASYVKRHAPRVKQSQRRHHATTETIREAD
                     HQGHHIVVKTRYEISVDGRKVLGHLGVSNDGHVHYHPIPNLSFASALDMVKAIIDVFP
                     HQFEAKKRPHKGPGRIRHTNRTHH"
     gene            22432..23388
                     /locus_tag="PLS35_03845"
     CDS             22432..23388
                     /locus_tag="PLS35_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HNI17819.1"
                     /translation="MAVTRRNILTNANVRAQYARGVNLLKREFTGPTTSSLGIPGPAR
                     QISTYDLFVVWHHTAMSTMTPPSQGDRNAAHRGPVFFPWHRFMLIELERNLQRVLGDD
                     TFGLPYWDWAADGSRTPGQQRQSALWRASGVGGTGSPVRTGPFRFVADDPDSFRIRVE
                     ADANGRLIQSNRGLRRDLGVNIATLPTRTDTAGALALTLYDQPNWDTTSAGFRNRAEG
                     WAAPDAPALHNRVHVWVGGDMLPSSSPNDPVFYLNHCNEDRLWEAWMARHTRAYVPTQ
                     SAPASLRGHRLNDQMLAFVSAAMRPADVLDVGSIYVYDSLAV"
     gene            23564..24304
                     /locus_tag="PLS35_03850"
     CDS             23564..24304
                     /locus_tag="PLS35_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI17820.1"
                     /translation="MAASQTYTVTILLDVNEAQDVDDRAESRSYLVADHSSSQAVIID
                     AVLENIERDVRILKELGLTLAYAIETHVHADHITAASLLKDRTGAQIVYGGGAAGTVM
                     GADLFLSDGQTLRFGGSALTALATPGHTDGCTSYLLPGAVFTGDALFIRGNGRTDLQG
                     GSAATLFDSVRQKLFALPDDTIVYPGHDYQGRVASTIGEEKRFNERLNLSIDKDTFIE
                     LMNRRKQPRPAKIDIAVPANLRAGRMAR"
     gene            24497..25015
                     /locus_tag="PLS35_03855"
     CDS             24497..25015
                     /locus_tag="PLS35_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17821.1"
                     /translation="MSHEQIAAPGPEWGIVIGSVLVRQEQKGSGADTAVRDQSTMFEV
                     EVVQVQPGDPNGESPYAARYHVDATAGLDRPFLARLRPGRYLIKNFYRTGMAGTGGDL
                     NLVFESQSGRVVYIGQLLVEVPPQLTHGKDYRFSVHDTHDITLSQLAPHHRDLTQQAT
                     TVPMQIREKDSH"
     gene            25208..25894
                     /locus_tag="PLS35_03860"
     CDS             25208..25894
                     /locus_tag="PLS35_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNI17822.1"
                     /translation="MSHNLKLSLCLNLILLGFILTGYVGPSVKTTFMPDVEAPVIGNH
                     TRIHPLGIVIGSVTLGDRVFVAPAASVRGDEGRHIHIGNESNVQDGVVVHGLETFEGD
                     HELPENEVEVDGMKYSVYIGNRVSLAHQSQVHGPARVGNDTFIGMQALVFRAEVGDRV
                     VVEPGAKIIGVKVPSGRYVPALSVVTTQDVADNLPLITERYPYRNLNGGVVRVNVQLA
                     EARQPQPIAR"
     gene            complement(25959..27167)
                     /gene="ilvA"
                     /locus_tag="PLS35_03865"
     CDS             complement(25959..27167)
                     /gene="ilvA"
                     /locus_tag="PLS35_03865"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HNI17823.1"
                     /translation="MVTVQSIAAAASRIASSIYESPLVHSRTLSRLTGNTIYLKLENL
                     QMTGSFKERGALNRILMMTEEERRRGVIAASAGNHAQGVAYHAVQRNIPVQIWMPRST
                     PLVKLSATRNHGADVVLHGENYDEACRAAMERSEERHATFIHPFDDDEVIAGQGTLGL
                     ELLRQNPTLDVIVVPVGGGGLIGGVGCAVKEQASNVEVVGVQTARLPSMKAALGSSIP
                     VELPASPTLADGIAVRTAGSRTLPLVQRYVDQMVTVDEDEIAAAILRLLEGEKTVAEG
                     AGAVALAALLQGNTGHRGKNIAVLVSGGNLDVNLLARIIERGMVRDGRRLRLRVLLPD
                     YPGSLEGLTSVIAKASANIVETSYNRAHYGVGLNEAALDVTMETRGREHASELLAALS
                     GSRYEFSVIE"
     gene            27639..29090
                     /gene="pyk"
                     /locus_tag="PLS35_03870"
     CDS             27639..29090
                     /gene="pyk"
                     /locus_tag="PLS35_03870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNI17824.1"
                     /translation="MSSSLVESTMQKTRIICTIGPATDSYEMLQKLYDAGMSIARLNM
                     SHGDHESHAKVIQHIKSLNRKVKFPIPILLDTQGPEIRTGDLSNELNLRQGEIVSVTT
                     RGPLNVEESSIHINYADLLDAVNVGDRITVDNGLINFEVLEKHERHMRCRVLDGGLLK
                     SKRHVNLPGVRVNLPAITPKDTKDILFGLERDVDFIALSFVREAGDIQQLKALMGDKV
                     GRVKIIAKIEDQEGVKNLESIIKESDGIMVARGDLGVEINLEDLPNVQRTIVRLCAEY
                     GKRVIVATHLLESMIHHPHPTRAEVTDVANAIYEEADAVMLSGETTVGKYPVKCVEYL
                     RRIALKSETIPGLQFAKRLRDVGNKQQLAAAAVQLAEGVKAKGIVVITRRGIMADLVA
                     NCRPFVTNIYAFTNMSQPRRTMLLNRGVFPFKIDFSSEPEKTLQTAFRILKDRERFQA
                     GDKVVIISDVLAQQRVDSIQIRDVPSDDLPVEA"
     gene            complement(29095..29541)
                     /locus_tag="PLS35_03875"
     CDS             complement(29095..29541)
                     /locus_tag="PLS35_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNI17825.1"
                     /translation="MPTNTVRLHRVLRSTPERIYRAFLDADAMAKWLPPNGFTGNVHH
                     MDAKVGGTFKMSFTNFTTGKSHSFGGKYLELTPYERIRYTDVFDDPNLPGEMHVTITL
                     KQMSCGTEMTAVQEGIPEMIPVEACYLGWQESLTLLTKLVEAEIPD"
     gene            29814..30503
                     /locus_tag="PLS35_03880"
     CDS             29814..30503
                     /locus_tag="PLS35_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HNI17826.1"
                     /translation="MNVRHRSITHACASITLTCGLVLVSLTGSGCQTAYYETMEKLGY
                     HKRDLMVSDVKKARDAQQDAKEQFKSALDRFTKTLNIQGGELQDKYEVLNSEYQQSEK
                     RAEAVRSRIASVEDVSDALFTEWEGELKQYSNASLRQKSQKQLAQTRTQYGQLIKAMK
                     RAEAKMDPVLAKLKDHVLFLKHNLNAQAIASLKSELTTVEGNIDLLIKDLNASIHEAD
                     TFIASMEKENA"
     gene            complement(30590..30985)
                     /locus_tag="PLS35_03885"
     CDS             complement(30590..30985)
                     /locus_tag="PLS35_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17827.1"
                     /translation="MDQQHQRSRGTGGGLFAGFLVGFLWFFLSFAAMFNGVFFLLLLV
                     PPAYALSGALLAWFFRLPPVHAACSLVGSALPWVAWLWPATMSSSGWMKSLVWPVFVG
                     TAFGMSLLGARLAAARRRRKPTQSDPALA"
     gene            complement(31040..>31236)
                     /locus_tag="PLS35_03890"
     CDS             complement(31040..>31236)
                     /locus_tag="PLS35_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17828.1"
                     /translation="RRSGGLAVHNVVIAVEGPEGAARQALFDAVDKGYLQGLVGQPLP
                     ELEASKARAGRDTQAASAAK"
BASE COUNT         6617 a         9243 c         8730 g         6446 t
ORIGIN      
        1 tgttcgtgct ggcgggtctg ttcgaaatcg gcggcggata cttggtctgg caatggtggc
       61 ggaatggtag ccactggagt gtcggcctgc tgggcgccgt catcttgatt ctgtacggca
      121 tcgtgccgac ctatcagccg tcgcacttcg gccgggtcta tgccgcctac ggcggctggt
      181 tcatcatcct ctcgattctg tgggggtggc tggtcgatca cgtggagccg gatcgattcg
      241 atatcatcgg ggcgatggtg tgtctggttg gcgtgacggt gatcatgtat tggccgaggt
      301 gagaggggga aagaggctgg cctggccgtc ctccatgctc gcgcaacgcg cggtcgaaga
      361 ctcccctcgt tggacgcgcg cacgcaggga accggccagg ccgcctcaga gggcgaatgt
      421 aatccaatca ataaacaaac aatagaccca gctagaacga aaaagtagaa tcctcagttg
      481 attatttggg agaactgtat gaaaggcgag tttaaggaaa tcggccactc aggaaataag
      541 atcacctttg agatattgac caatgctgag ggccaccgcg gctatcagac aagaattacc
      601 gacagctccc aggcgcctat agtcatgacc gaggcatacg cttttccagg cgggagaagg
      661 caaagctaaa tggccaatca ggacgagaca ttaaagcaca tctggaagtc tggctttcca
      721 tttcagttaa aaatcgagga tgaggttcga aggactcatt caactcacaa ttggttaatc
      781 gcaacgcgcg aacatccctg gacaaataaa gagactgaat cctccggctt cattgacctt
      841 gtcctgatgc acgatcactt cgtgacgtac aggatggtca ttgagtgcaa aagagtaaag
      901 gctgacgatc gaagacagct ccactggttg ttcttgattc ccgagggcga aaagcaagaa
      961 acaccgttgg cttcatgctt acagattggg ggaacctcac caatgaacgc gagaataggt
     1021 gaggcaaacg taagccaaca agaatgggat catgtgcgaa tatgggaaaa cgttaacatc
     1081 tttccaaaat ctttcgagtc ctcaatctgc gttcttccta gtgacgactc aaagagccag
     1141 cccatcttgg aacggctagc tgctggggtc attgactctg tcgagggatt aggggacgaa
     1201 gagattttgg tccaccaatc acaatttcag gtcgaccgct taatcgcctt cctctaccct
     1261 gtaatcgtca ccaatgcaga gatcgccatt tgcagttttg atccaagcaa agtcagcatc
     1321 ctagatggaa ctcttgatgc gtcggcagct accattacga cagtcccctt cattcgattt
     1381 cggaagagta ttgcaacaac attcccagaa ggcaccttct ccaatttaaa agaagcaaat
     1441 gaggccagag aactaaccgt atttgtcgta aatgctggcg ctctaccatc gctattgacg
     1501 aactggagga tggcatcgcg cgacctaagc ggacgatatg ccatacagaa ccacctacaa
     1561 cgataaccat ggccaagcct accctcattg tttgacctag aacacaatcc tatgcctacg
     1621 cacgatacct ttctttccta tgcgtccgag gacaacgatt ttgcctttca gatcgcatac
     1681 ggacttcgtg cgaacggtat ctcggtctgg tttgctccgc tcatgctcaa agttggtgat
     1741 agattacttg actctgtcga actaggcatg gctgagtctc gcacaggaac gctcctgcta
     1801 agtccaactt acctccacaa agggtggacc tcctacgaaa tggacatcct cattcgccag
     1861 cacatcgaag gaaccaaacc gctcttgccc atctggcaca acgcctccaa gaaacaagta
     1921 gatgcaaagc acagcgggct aggcgggatc gtcgctatca cagacacctc gtctgcgact
     1981 gaggtcgttt tcaagcttat agaaggactg tcgagcggtg cgcccagtag agccgtcgta
     2041 ccaatctggg aagatcccac ctataggttc ttagaaggtc tcgccgaggt gaaccttcaa
     2101 tccggggagg gggccgcaac aaccatcttc gagtttctgc tgcacgccaa ggagtcgcag
     2161 tacccactat ggatcggggg acgtgtcttc acgaagaacc agctgttatt gcacgtggcc
     2221 cagttgctcg gcccggtgcc tgatcgggtc aagagttggg ttcatgaaga cggataccgt
     2281 aagttgtggg agatgtgcgt tgagaacgat ctgaacccgc acaacttcta ctgagactag
     2341 ttgatactgc ggggctaacg gaccccttcg attgaataaa ccgccaccaa tgaagcaatg
     2401 ggtcatcctg agatggctcc ggtataacag ataggacaag gcctgtgatc acacattttc
     2461 ctacccggca cagagactcg catacggcac cttgcccatt tcttgccccg ctttttccag
     2521 agcagaggga cagcgagaat cgtgagcggc accagtagca agcgtccggt tgactggctg
     2581 caagggatgc gccgaggggg ttaggctttc gagtagtcgc gtatcgactc tgggtcgatt
     2641 gcgccgtggt cttcagttac aaaacagatt gctgagtggg cccactgcgg atttgctcct
     2701 ctctgtccac cagggcattc agttggtttc tcaattgttc atcttcaacc ttggcgacgg
     2761 cttctctggc accggcgaaa tcacctttga ggatcagatc cacgggcgcc gatagataca
     2821 ccaccatcat ctctccggat cgcccgagga gcttcgcgat ctcgatcgcc gcagtcttgt
     2881 ttcctatttt tgcaaaatgt cgggccaatt cggcatagct gtgaccttgc aaccaggatg
     2941 gattagggca cgagaggata gtctccttaa ttcctatgac gtccccgcga tcccgctgca
     3001 ggtggaggat atacatccac gcttccaaaa agtcttgccg cgccgcaatc ttggctccgg
     3061 tctgccgcgc gccaataaaa tcaccttcca tcgcttgaga gatcgcttcg tttttgagcg
     3121 cttcatccgg atggaaacga tcaggcatgg gcttcctcac ttgcgtcacc ttgctgaatg
     3181 acaccattca agcctagcac cctacgctga aaagtatagt agtactctcc ctgttctgcc
     3241 gtcggtctca tgtcctcaaa actgaccaga aatggtgccg ggaatcatgt atcggcagct
     3301 ggaacggaac acggcttatg caccatgcta tgtgcggccc aatatcaatg attcctgact
     3361 ggcctgccat ggaggaactg caacgatcaa cagtgcctga gaccacttta ctcacttgac
     3421 agagccctcg aaccactata gattccattt cggttactac ttagtcgaga tcaaaccact
     3481 caggttggac aatcggcacg ctaagagaaa ggtcttctat gaaaactgca tggatggctc
     3541 tgttggcatt atgcgcggtc gctcagaccg gcgaggcggc gttgatcgga acagatgtaa
     3601 tgttccaatc agtctttcag gccacctcca ctagtccaga atttaagcta acgagcctca
     3661 acaccgtgac ggtcaaggag ccaggcgtcg aattccccag cctcaaagcc ttgcaggtca
     3721 gtaatccgag tgggctccgc ttagttgata ttgcgatcaa tataggagac acgttcatcg
     3781 atatgaactt tactaattcc gcccctgact tccgattcgc atcaggcttc cgcaatggat
     3841 ataagctcac tttcgacaat gctgcaatgc ccagtatcag gcaggcactg atcgatcgta
     3901 ccgtgaccac gcttggctta tccgacagtg acgtcacatt ctccggcaat acgcttcaag
     3961 tcaacgtcga gagccttccg tttaacacct ccacgttcgc gcgaatcaat ttgacggtgg
     4021 aaggcggccc gagccccgtc cccgttccgg ctgcggcctg gctttttgga tctggccttg
     4081 ctgccttggt tgcgctgaaa aggagtggtg cttgatagat ggtcgagccc gtggcggcgg
     4141 gatcaaggcg acgccaagca gacagcgtgg cttagtgacc ggcccaggat gcggccacgc
     4201 gattgagtgg ctcgtgtgaa tcgctcacag accgcatagg agctggaatc actccaaacg
     4261 cgtgcgcaac ggggctcccg agcggtgaag atgcctgggt gcggaggttg gcgaagcagt
     4321 ctggaatgga atcgaccctc cggccacggg acgaccgaat gggttctgga agagctcagg
     4381 aagagaaaac ggggaccttc tgaatttctt ctgagttccg gaatggtgct cggtgacgct
     4441 cccccgcacg cagcgcgtat tgcccgcgcc tcgacaggaa cctcgccgat cttcagagaa
     4501 aggcagtacc cccgggctag gccgcgaacc gatcctgctg atcccaagcg agatgctcga
     4561 attcggcttg atccagtttg agatgcgcca ggaccttttc gccgatggca gcccactccg
     4621 gtgaatcgtt gcgtttccgg agtcgatggc aaatacagtc caccatcgcc agaaccgcga
     4681 tcacggtccg gatactttct tcggacgagg caagcttctg cgcctttccg ccgaacagct
     4741 cgcgagtccg gtgatgattg agaatgattg tgccgatttc ttccgggaga tgccaatgcc
     4801 ggctcatgag cgcccccacg accgcatgat cggtttttaa gcgcgcatct tcgatgtccg
     4861 gcagcgactg ctccggcctt ttcgccgcct cctgcagcac ctccgcatac tgcggaaggc
     4921 gttgcagcag aatcggcatg ccgcaatccc gaaagagccc gagcagatag gctttgtccg
     4981 tcgacatgac gggcacctca cgcatcacgc tcgacagaac catcgccgtg tcgttcgctt
     5041 cgtcgaagaa gcggtctatc cccgcttgat tgttcccctg tagttcgctc ttcatggcca
     5101 gggcgattac gacgttgacg acattgggca tccccagcaa caccaccgcc tggcgcaccg
     5161 actcgacctt tttcggcaac tcgaaccacg gagcgttgat ggttttcaac accgccgccg
     5221 ccacactcag atcccgctcg atgatgcccg ccacggccgc cacatcaggc tctgccttgg
     5281 cccgctcgcg attgatatca agcaacgtcc ccggttgagg cggcagcgtc accccctcca
     5341 ggagctgcat ggcagaatct tccgtcagtt tccgcaacac cgccgtcttg ttgtctttca
     5401 tcgcgtatcc ttgccgacat agaataatgt tctttatcgg ctcaaaaatg gatctctgta
     5461 gcccaattcc tcgccccgca tgaaaagata accagggacc ccctcaatga cttcagagct
     5521 ttagctcggt gtttggttat cctcccccac gcacatcgag tatttctcgt gacctaccgt
     5581 aagacgtttc actccactag tggattcact cttacatcac ggcgcttcgg cacgcgaatc
     5641 cggacgcctg gataagccaa cgtaccctcg ccgacccctg ctaagagaat cagactcccg
     5701 gataagacac caagagaaat gtcccgtaga gtgggaaccg ttgttgagca gaacaccggg
     5761 tgcggtcacg cgcacctgca ccaaggagga cgatatgagg accactgtac tatcatccga
     5821 gctggcccac tcaacgagtc gaatcggctt tatgcgttct gtctttgaac tgctcggaat
     5881 cgttctggtt cggttccggc ccgttcaaag aatgtggggc gcgtggctca ttgccgtcaa
     5941 cgcggcctgt ctgctgttca tcacgcacat tgaagctcag gtcgccctcg cagccgtagg
     6001 cctggcgcta ctcgcccagg cgcttatcta tcaacgcacg cgcttcattc ggctgctcgg
     6061 ggtgacacat ctgatctggg tacctatgct agcctggatg gccgtgcgtc ttgccgcggt
     6121 cccggaagag gacgtcgcgt tccaggcctg gctcctcgca ctcatcgcca cgaatgccat
     6181 ctcgttggcg atcgatgcct gggatgccac gcgtttcgtc cgtggcgaac gtcagcccca
     6241 ttacgtctgg tgatgaggag agaggaaagc ggaagaaagt gaccactcct cgccgggcta
     6301 agcgcgtctg acaaagactc gctcgatact gattgccttg agtgttgttg attgcgggcc
     6361 atgcccaatc tgcgggcaat cacggtctga caatcgcaca ttccgtctgc cgacgcagac
     6421 actcgaacca gacgtcgtta tttcgtcctt gcccggttct ttttccacac cagagcagca
     6481 gcgacgagtg taagtggcac cagcagtaca atcccaatcg agagactgta tgcgggtgcg
     6541 ccgagcaaac cggagtttcg ggaatcgtga tagtcgatgc cgcgagcaga actggtgact
     6601 cgcgcagaag agcggcaatg atcgagcgag cggtggcggg actaaggaga tgccaagctg
     6661 acagctcggc tcagtgactg actcgtgaca cggccaggcg gttgggtgac gcgtgtgaat
     6721 cgcccacaga ccgcatcgga gctgcaatca ctctgaaaca atgagcaacg gggccgcccg
     6781 aacagtgaag aagcctgttg cggcggatgg cgaaacagtt tggactggaa tcaagcagaa
     6841 acaaagttgc caggaattgc tccttcctcc acgctgccgt taccgccggc ggcgtggcgc
     6901 gattaaccac ggcatgacgg caaagagcag caggctgagg tagtaggccg cacccgaaac
     6961 cggatctcta gcgaacagca catcccgtgg cgtcatgcca cgaagcgcga tgcccacgac
     7021 aaactctgca ctcaagacca gtccgaacgc cagcagtccc acgacgatcc gcccccggtc
     7081 gtccacgccc gccaaaaaac gccgatcgat ccaacgagcc gccagcacaa tgacgatgag
     7141 catgaatggc atctccaaga gttccgctgc ccgttcgccc aggaccggaa ggaggagcag
     7201 gacgcggatt gtcccgagga gaaatccagc cccgaagacg attagaaaat aggcgaacgc
     7261 cggtgtgaat ccctgcatga ggcgctaggc tagctaaatg accattggct gtaaaggctt
     7321 tcgacgggcg cgtccgggcg atgtctgacg gaaggcgtac gatgccagga atcattcggc
     7381 ccgagcctgg tacccattcc ggctgcgacc tggctctttg ggtctggcct tgcggtgctg
     7441 gatgcactga aagggactgg caccaaggag tgcccttctg aagccggccc aagtcttctt
     7501 tcatccttgg cacctccggc ccatcgccca cgcaccaacc acatgcgtca cgcaatagac
     7561 caaacccacc ggaaacagta caatgctgcc ggggcctgtc ctgtgaaact gctggcgcgc
     7621 tgaggccgga gaacagaggg tgaggtggca cagtcgaagc aggttgcggc actgctcggg
     7681 ccgagcctca ttgtcgtctc cgttacagag ttgctgaacc tcgagatctt cacgaacacg
     7741 tccgcgcatc tcacgtacct caacggcact ttgcccgttg tcgcgggctt gactattgtc
     7801 cgtgcccaca attattggat gcctcgctgg cctgtgcgga tcacgctcac agggtggcta
     7861 ctcctcctcg gtgggctgat ccgcatggtc gctccagtct cgtcccaaca gaccgctcac
     7921 catcccacag ccgtattggc catgacggcg gtactgttgg tgtgtggagt cgtcctgacc
     7981 gtcaacgggt acagacaaag agacgtgagg gacagctgac cggcgcgaag agcagaagac
     8041 tgtgaacgcg agtcatgcta ccagggctca tgtctcaccc tagcaagaga acaacccatg
     8101 gacatacagt acaagaatga cacggagggt gtggactggg agaggatgtc cgagctcttc
     8161 gaacgcgtcg gttgggggaa acgcaaggcc gaagacattc aggctgcctt tcgatccagc
     8221 tcctatgttc gatgtgccta cgatgagcgt acgttggtcg gtgttggacg tacggtggat
     8281 gacggacaat actatgcatt gatcgtcgat ttggtggttg accctcagta tcaaggccgt
     8341 gggattggca gcaatatctt gtcgtatctc gaacactcac ttgccaacta tgtgtttacg
     8401 accctgacgg cggcggtggg caaagagggg ttctatctca accaaggatg gcgcagacac
     8461 accacgagtt ttatttggcc tcgcacagag cgacaaagaa aggatcacac gtaatctacc
     8521 aagggcccag catgatgccc caccagcgag gctgccctcg atgaaggcga gctgatgcgg
     8581 cgtccctccg gcgcgcggca gacagttcac cttgggcccc catcgcgtca gtcgagccag
     8641 cgatgaattc cctcaagacc ttccacagag tgtctgccgg tcaggagagg aacttcacgt
     8701 gcgaggtacc gaaccgtctc gcgagggtaa ccggattccg gcccaacgcc ggctggaggc
     8761 aagaactccc ctcgttactc tcccatagat cctcgaggct tgagcgcgta gaatggacac
     8821 atgaccattc cttagcgaac cagatccatc atgctggaac ttcggccaac ctgcgagcac
     8881 tgcaatacac cgttgccacc cgattcaccc gaggcgcgca tctgctccta cgaatgtacg
     8941 ttttgcgcgg catgcgtcac ggacgtgttg cacaatgtct gccccaattg cggcggcggc
     9001 ttcgaaccaa ggcccatcag accgtcgaag aattggagag gggagaacta tctcggcaag
     9061 gacccggcga gcacgacgat ccggcatcgg ccggttgatc cctccgccca tcgcgcattt
     9121 gccgccacgc tcaacgctgt cccacccgaa caacggtagc cactcgctcc aagacgacgc
     9181 caggaaatgg tcccaggaat catccagacc tggatacgta acaggggccc agccgaaccc
     9241 ttttgtcagg cgcctcatgc atattccgag aaggcgccga acctctctaa cacgtagagt
     9301 ccaagctagg acacgcaggt ctgcgagagt gaacgactgc aggcgcgtaa cctactcaca
     9361 cgcctgcctt gggagaatgt cagcggttca cgtaccatac tgatacacaa cgacacctgg
     9421 ccccatgcaa cagacaacgg gccaggcacc atttttttct ccaagaactt ccccttcagg
     9481 ctcccctcat tcgcgtattc cgtttgtgat cagtggatgt tcctctataa tttcacttag
     9541 gggtgcggag gccgggccgt gtagtgatgt accggatgga ggttgccatg gtgtctgttc
     9601 cgctgaccaa tctcaaaaat gattcgatca actccgacga cgccatcctg ctgttcgaaa
     9661 acggtggcca caaagtctat tggatcggca cacacaccgg aggagacgag atcgagtgca
     9721 acacctatct gatcatcgac ggacacgagg gctacatcct tgaaccgggc gggttcgacc
     9781 gtttcccgca tgtgttcaag aaagtgtgtc tcctcctcag cccgaatagc atcacccacc
     9841 tgttcttcag ccatcaagac cccgatatct gtgcctcatt tcccagttgg acaaagtgga
     9901 atgacgacat caagctggtt gtgtcgagct tatggacccg gttcatgctg cattatgagt
     9961 ccagggacct gagcgggaag gcccacaacc tcaatttcgt caaagtaccg gacgagggcc
    10021 tttcgatccc gttgaatgac ggcggccgac tcgattgtct ctccgcgcca tttctgcact
    10081 ccccgggcaa tattcttgtt cacgatacgg tcagcgggtt cctgttttcc ggagacgtcg
    10141 gcgcagccat gtacaaggac cacaccttca ggctcgtgat tgatgattgg aacgcgcata
    10201 cccaagccat gcagggattt catcagcgct atatgtccag caatcgagct gtcgccggga
    10261 tgaacaaaca actcgcgcca ctgccgatca cggcaattct tcctcagcac ggatgcattt
    10321 ttcgcggaga cgaggtttca cgcttcaccc aatggctcgg taaactcccc gtcggagccg
    10381 actatttcta ccccgacctg gcgtgactcc tacgggccgg ggttggtgcc gctgcaaccc
    10441 ggcctgacgg tggtccatca tccgccacgc ctgctcaact atacgcatca agtcatggct
    10501 gcgattcagt atcgatcggg caagcccacg gctgcagtgc gagctgaacg cggcgtgaat
    10561 actcttgcat ccatctcccc actgcagaag cagatctgat agtatccgcc acaatcgaca
    10621 aggctccttc taaccttgtc ccgttaacac aactgcgcat tccctgagaa acaggaacac
    10681 atcggagtac tcgttcgtgg atgacaatgc cctagagaac acagccatcg tcacttcgca
    10741 gacaggcgtg gtggcacgat ggcagcagat cccgctgtat ggacgcatca tcattggcgt
    10801 gatactgggc cttgcagcgg ggctcgcgct gggaaaccag gccgcctttc tagccacgcc
    10861 gggcaagctg gtgctccgcc ttttgggggc gctcgcgccg ccgctgattc tggtcgcgat
    10921 tgtccacacc ttcatgacga cgcatctcgg cggcccgctg gccgcacgcc tcccccgatt
    10981 gctgctgatg aatacgttgg tggcgatcac tgtcgggctg accgttgcca acgtcatcca
    11041 gccaggacga ggtgcgggac ttgcgccagc agaacccgcc gtcgaaacca cgcatacggc
    11101 aaatccactc gtggcgttta ttgagaatgt gcccaagagc ctgctggggc cgcttggtga
    11161 cgatggaaaa gtgatcggag tcatcttcgt cgcggtggcc ttcggcatgg ctctgcgtaa
    11221 ggagcgccat cgcccgctcg gaacggtcga gcatctcgtc gaactggtgc tagagtcctt
    11281 gatcaaaatt ttgcactgga tcatcgcggt ggtgcccctg gcggtgttcg gcatcgtggc
    11341 gagcattgta ggaacagagg gtttcgcgca gtttcaggcg ctgggtatct ttgtcctcag
    11401 cgtgttgctc gcgctcacga tccaggctgc ctattatctc gttcgcgttc ggttcgggtc
    11461 ctgggtccgt ccgggtgagt tgcttcgtgg cggacgggat gcgctggtca tggcgttttc
    11521 cacggcgagt tcgacggcca cgatgccggt gacttacgcg gcgctcaaag atcgcatcgg
    11581 cctgcgcgaa caatccgcca gcatgggcgc actggtcggg gcaaatttca acaacgacgg
    11641 caccgcgctt tacgaggcca tggcagccct gtttattgcg caaatgatcg ggatcgatct
    11701 gagcatgcac caacaagtga tgatcgtcct gacgagcatc attgcctcgg tcggcgcagc
    11761 cggtattccc gaggcaggtc ttgtcaccat gacgatggtc ttcacggccg tcggcctccc
    11821 cgtccagttt attcccgtcc tgctcacggt ggattggttt ctcgatcgct gccggacggc
    11881 gatcaatgtc atgggtgaca tgaatgtgag ttgcctgctg gatggaaagc agaaaggatg
    11941 aatcagcgca aactctgacg aaggatcaac gtcgctgcgc gcgcggtgcg ttctgcactc
    12001 gctccgaggt atgtttcgat ggccacaagg tcttcagcgc gagtcccgcg ccgacgagtg
    12061 cggcccctgt cagcatggga tgcagggatg cggtggtgta gagactcgat tccgacacat
    12121 ggccctgata gtcgcctcgc tcccgcaaat ccccggacgg gcggtccaag gcgttgtccc
    12181 ggcgaggcct ggcgggggga cgatccgact gttgttgtcg ggcaaacaat gtttccatca
    12241 gcaggtcagc cagcctgggt gcgtagtagc ccatcatgcc gatgcctttc ccgctggccc
    12301 cgacgtagac atggcgctct ctgttttcgg cgcagtacag aatggtctgg gcagccgtct
    12361 cgggtgaata cacgggcggc ggattcgtgg gctccgtctt catgtaattt ttggcatgtt
    12421 gtgagtaggg cgtcgcaatg gcgcccggtt gaatgagggt gacggagatc ggaataccat
    12481 ccatctccac ttccatgcgg agcgcatcgg taaatgcttt cacggcatgc ttggaggccg
    12541 aatacatccc ctgcaacacc agggcgcggt ctgagagggt actgccgacg ttaatgatcg
    12601 ccccggcctg atcgtgcgct ttgaagtacc gcgccgcttc cagtgacccg tacaccaatc
    12661 cccagaaatt ggtctcgaac aatcgccgca tatcttcgat cggttcatcc aggagccgtc
    12721 cgtacatcga cacgcccgcg ttattgatcc aggtgtcgaa ccctccgaac atctgtcgcg
    12781 caacacgtga tatccgttgc acatcggcgg ccttccccac gtctgccgtc acggtcacgg
    12841 cccgatgctg tccctgtgac aattcccgct cgagttcagt cagcgctcct tctgttcgac
    12901 cggccagaac gagtcgcgcc cctcgtcgag ccgccatccg cgcggtggtc aggccgatgc
    12961 cggatgtcgc cccggtgatg acgatgattt gctgacgaag tggtttgagc gagaaggcca
    13021 tcatgagagt tcctttctct cgttgtgtcg gtatgccgat cgcgtgctgg cgtctgtgtg
    13081 tggaggtctc tgcgtggatt gagaacaggt cgcaaaggta ggccgaaccc gccatcgatc
    13141 cgacgctatt ctacgcattc tttacgtaag gccgactggt aatacccctg gaatcgcgag
    13201 aaccggcgaa cgccgtcgca gatgttgctg aggagacctg aaccatccct tgtaggagtt
    13261 catccatcgg agtgatcgac ctcagatacg tcggggcgga cgccagcgca tctcagggtc
    13321 tggattgctc cattgactcg attggtacgc aggagtaggg tttcatcgct cttccgaccg
    13381 acctttcaca gacttgagta ggtgaccgtg catgccgctc tgccagccgg atctttcatt
    13441 attcaggagg aaatggccta tgtggagatc acgtatagca actgctgcac tcgccgtggc
    13501 gctgataagc ccgctttcag tccttgcgga agaacctccc gccaatccaa aatccctggc
    13561 cgagcagctc gtcgatgcct tcaacggggt cttcggagtg catccaggta tgcgggccaa
    13621 tcatccaaaa gggatggtcc tggaaggcac gttcacgccc accgcatcgg cggcctccgt
    13681 cagcaaggcg gctcacctgc aaaagaagaa aaccgctgtt ccggtgacgg tgcggttctc
    13741 tgccggttcg gggcaaccgt ccgttccgga caccagccag atgcctcgcg ggatggccgt
    13801 caaattttcc ctgcccgacg gatccaagac ggatctcgtc gtcctttctt ttaacggatt
    13861 tcctgtggcc acggcggaag agttcagaga ctttctcctc gcggtcgggg caagcggacc
    13921 cgatgcccct aaacccactc ccatcgagca gttcctagcg acgcatccgg cggccaaaca
    13981 tttcgtggag agcccgaagc ctcaaccggt cagttatgcc accctgccct atttcggcat
    14041 caacgcgttc aaattcacga atgcgaaagg gcacgtcaca ttcggtcgat accagcttcg
    14101 ccccatggcg ggcgagcact ttctcaccca ggaacaactc aacacgatgg acccggatta
    14161 tctgagcgcg gagattcgcg aacgattgcg ccgtggtcct gccaagttca cactcctcct
    14221 gcaagtggcg gaaaagggcg acaagatcga cgatccctcg atcgcctggc cggatagccg
    14281 gaaacagatc aagttgggca cgatcagcat caccaaagcg gtggcggata gtcaggctgc
    14341 cgagaagaag ctgcttttca tgccgggagc gttggtcccc ggtatcgaag cagccgatcc
    14401 gatgattgcc gctcgttccg cctcgtatgt cgtgtccctg tcacggcggg cccaggcgca
    14461 atgaggctgg cgaaacactc gatggatcgc ggccctgtcg gaaatcggcg cgcagtacta
    14521 ccagctaacg caccgacgtg gtgatggcac gctggtcccg ggtatggctg gggcctgaag
    14581 gactgtggtt gatgatcagt ctcgcggtgt atgtcgccgc cgcaatgaat caaccgtcga
    14641 cgccggcggg gaacgacttc cttgaaacgc tctgggtagc cattccatta gtcggcattc
    14701 ccctcacctt cgcgacggcc ttgttgccta gaggctcagg ctggtggtgg ctgacccgtg
    14761 tggtcgtcgc ctcatgcatc ggtgtgatag tcgcttcctt catcgcagca ggcggagtgg
    14821 actatcacga ttcccgcaac tccggactct tgggcgcgcc cgtctacagc ctctcgatcg
    14881 gactgctgct cctgtttccc ctcaccattc tcgctatcct gctgatttgg aagaaacgcc
    14941 gcacgagccg ctgattgcct tagaattatg gctacatgat gtttggatta tccacgggaa
    15001 cgcgctaggg ctccgcccag tagactagaa tcctgctggc agtacgattg cggtgaggag
    15061 aacttgtgac ggcatgacgc tgagcctccg attgggctga ccctcagcgc tgcacgagcc
    15121 tcagtgaacg aacggacctg ccggtgaatg ggatagacaa ctcgatgact ctctccagga
    15181 aaacgattca cgatagtacc ggcctgcttg gagagttgct ggcgcagtat cacccccgga
    15241 attttgatan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtaccggcct
    15361 gcttggagag ttgctggcgc agtatcaccc ccggaatttt gatatccgtt tgtgggacgg
    15421 gacaatctgg cctgccgatc ccggcgcgac tcccgaattc accctcatcc tccgcacccc
    15481 aaccgtcctg agggacatgc tgtggaaggc cagcgagctc gcactagccg aggcgtttat
    15541 ctccggtgat ctggagatcg atggagatct tgaacaagcc atccctcttg cggaatacct
    15601 ctcgcgtcat cccgtgacct gggggagttg gctgcgattg gggttcaaat cattacgttg
    15661 gccatcgaac ccctccttca ccacgaatgg cctggccgca cacgtcaccg ggcccaacca
    15721 ttcactcctt cgcgacaaac aagccatcgc gtatcattac gatcatcccc cggaatttta
    15781 tgccgggtgg ttggatccgc agatggtcta ttcatgcgcc tacttctctt tcaccgacga
    15841 tctcgatgcc gcgcaggtac acaaactcga ttatctctgc agaaagctcc gtctccgacg
    15901 tggcgagcgg ctgctcgatg tcgggtgcgg ctggggcgca ctgatcgttc acgctgcccg
    15961 atactacggg gtccaggcct gcggcattac cttgagcgag aagcaggctg aatttgcaga
    16021 ggcctgcatt caacaacatg gactcatgga tcgctgtcag gtcgaactcc gcgactatcg
    16081 acaactgagc actcccaatc gctatgacaa gattgtcagc gtcggtatgg tcgaacatgt
    16141 gggagccact cacctctcag gctattttcg ccatatctgg aatttgctga agccgggcgg
    16201 aacctttctc aatcatggga tcgggattcg gcatggggaa cgctgcgcat tcgggccatt
    16261 cgtgaatacc tatgtcttcc ctgatgcgga attgaccacc atctcgacca ccaccggatt
    16321 cgctgaaacc gccggctttg aagtaagaga tgtggagtgt ttacgagagc attacgtcct
    16381 gacccttcga caatggagac agcgcctgga gaagaacgtg acgcccggat cgtccagcat
    16441 ggatgaatcc acatggagat tgtggcgcct gtatctcgcc agcgccgccc atggctttgc
    16501 cacaggcaag ctcaacctct atcaaacctt gctgtcgaaa acgcaggagg gatcggccgg
    16561 cctgccatta acgcgggatg actggtatga ggcccattcc tcgattgatt cgcgcctgtc
    16621 gggaagactg cgttagtccc gccctctgaa taagacttct ttcctccctc ctcacattgt
    16681 tgctcttgag aagaacaccg cgtagctcga atgcgcaaaa tacttgctgc gtccatgcag
    16741 gaccgctagc ctgaccttac aacaccaaca cgcggctgat cgggttggtt tggagaggaa
    16801 agtcatgaaa cgggatacct ggcttgtcgt gacggcggtg actggcggaa tcgtcgtgtg
    16861 gttgttggtc tcggccttcg ctcacaagag agaggcgtgg gattccgatc tgtatttcac
    16921 gctgggcctg cccgcggtct gcctgctgtc tgctctgctt ggctaccttg aaccgaacag
    16981 ggcctggcgc tggggagcgg cgcctcttgt cgggcaggcg cttgtgatgc tgctgactca
    17041 aggcccgggt actgtgttgc cgctcggaat catgatgttc ggcgtccttg ccctaccgtc
    17101 gatcgtcacc gcaaagatgg gagcctatgc ggccaagaga agtctccacc gatcaaccgc
    17161 taggcagaac cattgagccg atcaccttgc cggctcctgg tcatcgtttt tttccagttc
    17221 gcgccgcaat gcgtgactca gccgataccc agccggcatc gaaaggatgg ccggctgtat
    17281 ccagatcaat gaccagcgga gcatgatccg aaggcaacgg ctttcccttc cgcgcctcgc
    17341 gatcgatttc ggaccagatc agacgatctc tcagcggagt gctcacgagc aggtgatcga
    17401 tgcgcatgcc gatgttcttg tggaaattgc cggcgcggta gtcccaccac gtgtaccgcc
    17461 ccggttccgg gtggtggcat cggtacgcat cgacgaatcc ctcgcgtacg atccggtcga
    17521 atgcacgccg ctcacgatct gacacgtgcg tgccgccgtg gcaggcggcc gggtcccaga
    17581 cgtcgatgtc ctgaggcgcc acgttgaaat cgcccccgat gacgcaggct ttctgcggtg
    17641 cacccgtctg cttgagccat cgcgccagct tgtcgaacca gaccagcttc gcctcataaa
    17701 acggagaatc gacctcacgg ccattcggtg catacaggca gataatgagc agtcctccgc
    17761 aatcggcagc caacatcctg gcttcggcga acggctcgtc gtcgcccgtg tcagtcgaac
    17821 gtgccggctg caacggctct ccgaaattgc tgatgacgcc gctgatgcca cagcggctgg
    17881 cgatcgcgac gccgttccat cggccttcac cgtggtgcgc cagcgcgtat cccgcctgtg
    17941 cgaacgcgtc gatcggcgca tcggcatccg acaacttcgt ttcctgcatc agcaacacgt
    18001 cgggcttggc ccgatccagc caccacagga ccttctcgag acgcgccttg agtgagttga
    18061 cgttccaggt cgcaatccgc atcccttaca gtaccctatt tgaatggacg tgtgccgaag
    18121 aaacggtcgg tgggtcggtg ggtgcttggt cactgcatgc tgacgacaat cccttcgtcg
    18181 ggaccgagaa gaaaatggtc tgccgtccag ggcccttcgg ctctctgggg gtcggtggaa
    18241 agcatcagcg tgccgtgacg ttcaagattg gaagggcccg ccggcagtga gatcttctgc
    18301 gtgtgggcgc tgaagttgac ggcaataaac agagcttctc gctggccatc cacgaggctc
    18361 tctcgtgtga agaacaggca ttccggtgga actccatcga taggatgata ggttccgaca
    18421 gtgagggcgt tcgatccttt cttcatccgg ataagccgtt tgtacaggga caagatcgaa
    18481 gcgggatcgc gctgctcccc agccacattc gtctgccgcc atgcgtgtga aagtggcaac
    18541 cagggtctgc cggtcgtgaa accggcttga gggctgtcgt cccagggcat gggagttcgc
    18601 gcgggatcgc gtcccgtttt ccacggccaa tagcggatgg tgtaaggatc gtgcaggtcc
    18661 gctcgcctga gcctgccatc atgaagaccc aattcttctc cgtaatagac gaatggcgta
    18721 ccgcgaagcg tgaacaacag cagggctgcg gccctggcgc gccggatagc ctcgccgccc
    18781 gcgccgtacc gatcgatgtg ccgcgactgg tcatggttgc tgaacaccac cggaggccag
    18841 ccaccctgtg ggaccgcgcg atcgctgtcc aggatgagcc ggcgaaaggt gtccggatgc
    18901 caatcagacc gaagcaagcg aaagtcgaac acctcgtgca gttcgttcga tccattcccg
    18961 taaaatgccg ccggcccacc aggagtatcc gaggacgtct ccccgaccag cagaatgtcc
    19021 tctcgatcct tgagtgagga ccggagtgcc cgcagcgcct catgggtatg cggctggtca
    19081 cggtcgtagc gatggatttg tcgataatag ctgcgccacg ccagacggcg cggattgtcc
    19141 ggccagctgg tgtctttccc caaccaattg atcgcatcca accggaagcc gtcgaccccg
    19201 cggtcgagcc agaaccggac gacaccgaag accgcgtcgc ggacggcagg gttggtccag
    19261 ttcaggtccg gctgctccga caaaaacgaa tgtaagtagt actggccggt gtgcggatca
    19321 tgtgtccatg ccgatccacc gaatcgcgca gcccaattat tgggaggccg ccggccggat
    19381 ttgccgtcgg cccagtagta ccagtcgcgt ttcgcggagg ttctggacga acgcgattcg
    19441 atgaaccagg gatgctggtt cgacgtatgg ttcaggacca gatccatgac gactcgtatg
    19501 ccccggcggt gagcttccga gacgaggcga tcgaagtctg ccagcgtgcc gaagcgcgga
    19561 tcgatatcgc agtagtcact gacgtcataa ccgaagtcat gcatgggcga aggataaatc
    19621 ggagagagcc agatggcgtc gacgccaagc gagtcgggcg agccgttgag ataatcgagc
    19681 cggctgatga tacccgggag atcgccgatt ccatccccgt tcgaatcctg aaaagaccag
    19741 ggatagatct gatagacgat ggccgattgc caccagggag tgttgggctg caggagggtc
    19801 atgatctgcc ttcgacccta cctcaggttg ccccgaggta caagcagagg gcgaaaattg
    19861 cgacgacagg accaagacgg cagccgcgcg gccacgaagg ccacgggaaa tgtcaccgga
    19921 agctcgcgaa atccggcagc cacttagctt cccagcaata cgcctctcgc tgagacaatg
    19981 acagtgacac tcagccgaca tgcgctgaag aaacgctgtg cgttcaacag agtacccgtg
    20041 atgaaaccag aaccgccgat ggtggcgtag gtcgtcaccg ccgttggagc atcgactctg
    20101 gaaatcgtcc cccgatctac cttttgcttc ctcttcgcac cagttccttt tttccgcgtg
    20161 gcagatggaa gtcacacatc aagcggctga agaagtctgc gccttccagc cgagcgagct
    20221 catccgggac cgccacttcc ttcaacgact gtcccttcat cggctcgatc tgaatatgca
    20281 ccggtttgcc cttatgatct tttccgggag tcagtaggcc tccccccacg atcgccccct
    20341 ttttgttcgt cagcacatgc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnacttgatt gaatgccata acaggcctcc cttaaaaagt tcgggcgcat tatagcgaac
    20521 gagcacgcgc gatgtaatcc agtcatccgc gattcaacag tggaattctt tccagcggga
    20581 actcacaata ttgccgagga agggtcaacc cgggcagacg atgacgacga tcgatcccac
    20641 cagacatgaa tgggcgaggt ttgagcgcct cattgtcttc gacggcatct gcaattggtg
    20701 caatgcctgg gttaatttca cgatggctcg tgaccgtggg cgctttcact tcgcgacgtt
    20761 gcaatcggcg aaggggcagg aactgctcac gctgttggga ttgcctgtcc aggatttcga
    20821 gacgtttctc ctgctggagc ggggtcaggt gttcaccaaa tcgaccgccg ctttgagaat
    20881 cgccagaacg ctgtctggct ggtggccgtt gctgtatggc tttattctta taccccgcgg
    20941 actgcgagat attctctaca actacgtcgc acgccatcgc taccggctta tggggaaggc
    21001 tgatacctgc cgcatcccca gcacgacaga ccgggaccgt ttcgtctaag cggattgccc
    21061 gccgattttt ctgagtgcgg tccccttgtg catcgccaga tggcctgttt tgtcgctttt
    21121 cacgagatat tgcggctgcc ccttggaagc tcgggcgata tacgacttga acttcatggg
    21181 agccgtgatc cttttctgaa ttgtccctcg aatacgcccc gcttccgaat tccaactcac
    21241 atgatcgccc actttaaaat tttgatccat ggcaactcct ccggctggcg tgagtacgca
    21301 ttgtgccccc tcgttacgca tccccatact gggatggtgc ccaggtacgc ctctcgccgg
    21361 cacgcccggt ataatgaacc agttgctctg aacccaggag gggtctcgac atggatatcg
    21421 tgtcgcacgg tctgtgggga gcgttggcgt ttggtcgaag aagtcgcacg agcttctggc
    21481 tggcgttcgg aatcggcctg gcgccggacc tgttttcgtt cggcatcttg tgggcagctg
    21541 cgacgcttgg cctgtcgccg aagcctgatt tcagccacgg cacgccaccg gagtccacca
    21601 ttccgcccta cgtgcatcat ctgtacgatg tcacacatag cttcgtcgtc ttcctcgccg
    21661 tctttgccct gttgtggctt gtcatgaaac gaccggtgtg ggagcttggt gcctggggtt
    21721 tacatgtctt agtcgacatc ccgacccatt cgtttgcgtt ttttccaacc cccattttct
    21781 ggcctctctc cgattggaaa ttcgatgggt ggcaatggag ccagccggcc attctcatcc
    21841 cgaacttcac agtgctgttg ttgctctacg gttggtttct gttccaacag gcacggccga
    21901 ttctcaggcc cgaacgggcg cgtgactgac ggtccgtccc ctacgaagcg ggcacccgca
    21961 ggccctctgg accagcgcga gctaccgccg tacactgata tcgttcagcc atctggatag
    22021 gtgagaggaa ggagtgaact tatggccaat agaaaacgcg agccggtgcc gacgccccag
    22081 tccatcgcgt cgtatgtgaa gcggcatgcc ccgcgagtga aacaaagcca gcggcggcat
    22141 catgccacca ctgagacgat ccgcgaggcg gaccatcaag gacaccacat cgtggtgaag
    22201 acccggtacg agatttccgt cgacggccgc aaggtgctcg gtcaccttgg cgtctccaac
    22261 gacgggcacg ttcattatca ccccatcccg aatttgtcgt tcgcctccgc gctggacatg
    22321 gtgaaagcca tcatcgatgt gttcccccac cagttcgagg cgaagaaacg tccgcacaag
    22381 ggaccgggac gcattcgcca cacgaaccgc acacaccact gaggtgaact catggccgtg
    22441 accagacgca acattctcac caatgccaac gttcgtgcgc agtacgcccg cggggtgaac
    22501 ctcttgaaac gtgagttcac gggtccgacg accagcagtc tcggtatccc cggtcccgct
    22561 cgacagatca gcacctatga tttattcgtc gtctggcacc atacggccat gtcgactatg
    22621 acgcccccgt cacagggaga ccgcaatgcc gcccatcgcg gccctgtgtt cttcccctgg
    22681 caccggttca tgttgatcga gctggaacga aatctccaac gagtgctcgg cgatgatacg
    22741 ttcggacttc cctactggga ttgggccgcg gatggatcca ggacaccggg tcagcagcga
    22801 caatctgcgc tttggcgggc atcgggtgtc ggcggaacag gctcaccggt tcggaccggt
    22861 ccctttcgct ttgtcgccga cgatcctgac agctttcgca ttcgggtcga ggctgatgca
    22921 aacggccgac tgatccagag caatcgcggc ttgcggcggg atctgggggt gaatatcgcg
    22981 accctcccca ctcgcactga tacggccggc gcccttgcgc tcactctcta cgatcaaccc
    23041 aactgggata caacatcggc cggctttcgg aatcgagccg aaggctgggc agcgcctgac
    23101 gcaccggcgc tgcataaccg cgtgcacgtg tgggtcggcg gagatatgtt gccgtccagt
    23161 tcccccaacg atcccgtctt ctatctgaat cattgcaatg aagaccgcct gtgggaagcc
    23221 tggatggccc ggcatactcg cgcctacgtt ccgacgcaat ccgcgcctgc cagtctccgc
    23281 ggacaccgcc tcaacgacca gatgctggca ttcgtctctg cagcaatgag acccgctgac
    23341 gtgctcgacg tgggaagcat ctacgtctac gattccctgg ccgtatgagg ggaaggcctc
    23401 tttcgagaac ccagggagag ccacatcttc ccaatggggc cagcagccct cagtcactca
    23461 cacgcctgat cgaattgcca ggagccatgg accggtgcca tgcaaccggc tacaatgcgc
    23521 cggtttcttc gggtcgctct tccttttatc tcggagttca tccatggcag cgagccagac
    23581 ctataccgtc accattctgc tggacgtgaa tgaggcgcaa gacgttgacg atcgggcgga
    23641 aagtcgcagt tatctcgtag cggatcacag ctcaagccag gccgtgatca tcgatgccgt
    23701 gctcgagaac atcgaacgcg atgtccggat cctgaaggaa ctgggcctga cgctggccta
    23761 tgcgatcgaa acccatgtgc atgccgacca catcaccgcc gcgtcgctcc tcaaggatcg
    23821 caccggcgcg caaattgtct atggtggtgg cgcagccgga actgtcatgg gagccgacct
    23881 gtttctttct gacggacaga cactccggtt cggcgggagt gccttgacgg cgctggcgac
    23941 gcccggtcac acggacgggt gcaccagtta tctcttaccg ggtgcggtgt ttacaggcga
    24001 tgcgctgttc attcggggga atggtcgaac ggatctccaa gggggatcag cggccacgct
    24061 gttcgactcg gttcgacaga aactgttcgc actgccggac gacaccatcg tctatccagg
    24121 ccacgactat caaggccggg tcgcttccac catcggcgaa gaaaaacgct tcaatgaacg
    24181 gctcaatctc tcaatcgaca aagacacgtt catcgagctg atgaacaggc ggaaacaacc
    24241 aaggcccgcg aagatcgaca ttgccgttcc ggcgaatctg cgggccggtc gcatggcacg
    24301 atgagcacga gccgcgtggt cgtcggcgcg cgtacccacg cagacaacgg acgagcgccc
    24361 gtcttgactg agagagccca accgaagaga gtgaggatgc atgcgccctg actgtacttc
    24421 tctcccgaaa cctgttgctt ccattctgct tgcggctctg gcctggagcc tcagctcctg
    24481 cgcgtcatca ctagacatgt cacacgaaca gatcgcggca ccgggtcctg aatggggaat
    24541 cgttatcggg tcggtgctgg tcaggcaaga acagaaaggc tccggagccg acacggcggt
    24601 acgcgatcag tccacgatgt ttgaggttga agtggtgcag gttcagcctg gtgatcctaa
    24661 tggggaaagt ccctatgcag cccgatatca cgtggacgcc acggcgggcc tggaccggcc
    24721 gtttctggcc cggttgcgac ccggacggta tctgatcaag aatttttatc gcacgggcat
    24781 ggccggcacg ggaggcgacc tcaatctcgt cttcgaaagc caatcaggcc gcgtggtcta
    24841 tatcgggcaa ttgcttgtcg aggtgccacc gcagctgact cacgggaaag attaccgctt
    24901 ttcggtgcac gacacccacg acatcaccct ctcccaactt gctccgcacc atcgcgatct
    24961 gacacaacag gccaccacgg tccccatgca gattcgcgag aaagacagcc actgagatat
    25021 ctgagcccac cacacagcat ctagaacgcc tgccggcaca gcctgcagac ttcctgctct
    25081 ttcaaatctc cttcatttcc ttgtacattc agggtcccac gcctacactt tcacctatgg
    25141 aatacctaga gtcttctcag acagaccact gcatgacgag gctttcgttc gaaagaaagt
    25201 tggagctatg tcgcacaacc tgaaactgtc cctctgtctg aacctgattc tgctcggttt
    25261 catccttacc ggctacgtcg gtcccagcgt gaaaacgaca ttcatgccgg atgtcgaagc
    25321 gccggtgatc gggaatcaca ccaggattca tcccctcggc attgtcatcg gatccgtcac
    25381 cctcggcgat cgcgtgtttg tcgcgcccgc cgcgtcggtg cgcggtgatg aggggcggca
    25441 catccacatc ggaaacgaga gcaatgtgca ggacggcgtc gtggttcacg gcctcgagac
    25501 cttcgaaggc gaccatgaac ttccggagaa cgaagtcgaa gtcgacggca tgaagtattc
    25561 cgtctacatc ggcaaccgtg tgtctcttgc gcatcagtcg caagtgcatg gtcccgctcg
    25621 agtcggcaat gataccttca tcggcatgca ggcgttggtg ttccgtgcgg aggtgggaga
    25681 tcgtgtcgtc gtcgaaccgg gtgccaaaat tatcggggtg aaggtgcctt caggacggta
    25741 cgtgccggcg ctctcggttg tgacgaccca ggatgtcgcg gataacctgc cactgatcac
    25801 ggagcgctat ccctatcgca acctgaatgg gggcgtggtc cgcgtcaatg tccaattggc
    25861 agaagccagg caaccgcagc ccattgctcg ctaggaaggg acgcggcgcg cggcgccgtg
    25921 aattgagcat cagatcaatt caacggtcat cccgtcgact attcgatcac gctgaactcg
    25981 taacgggagc cgctcaacgc cgccagtagc tccgacgcat gttcgcggcc acgtgtttcc
    26041 atggtcacgt ccaacgccgc ctcgttcagt ccgacaccat aatgggcacg gttgtacgat
    26101 gtctccacga tattcgcgct ggccttggcg attacggagg tgagtccttc aagcgaaccg
    26161 ggatagtccg gcagcagcac gcgcagccgc aaccgccttc catccctgac catccctcgc
    26221 tcgatgatgc gggccagaag attcacatcc agattcccgc cggacacgag caccgcgatg
    26281 tttttgccac ggtgccccgt attaccctgt aacaatgccg ccaaggccac cgcccctgcc
    26341 ccctctgcaa cggtcttctc tccttcgagg agcctcaaga tcgcggcggc gatttcatct
    26401 tcatcgacgg tgaccatctg gtcgacgtag cgttgcacca acggcagcgt ccgggagcct
    26461 gcggttctca ccgcgatccc gtccgccagg gtcgggctgg ccgggagttc gaccggaatg
    26521 ctgctcccca gcgcggcctt catcgaggga agacgagctg tttgcacgcc caccacttcc
    26581 acattgctcg cctgttcctt caccgcgcag ccgacgcccc cgatcagccc tccgcctcct
    26641 accggcacca cgatcacatc caaggttgga ttctgccgca acagttccaa gcccagcgtc
    26701 ccctgtccgg cgatcacctc gtcgtcatcg aagggatgga tgaaggtggc gtgccgctcc
    26761 tcacttcgct ccatcgccgc ccggcacgcc tcgtcgtaat tctctccgtg cagcacgaca
    26821 tccgcgccat gattccgtgt cgccgagagt ttcaccaggg gcgtcgaccg cggcatccaa
    26881 atctggacag gaatgtttcg ttgcaccgcg tgataggcca cgccctgggc gtggttgccg
    26941 gccgacgccg cgatgacccc gcggcggcgc tcctcctcgg tcatcatgag aatccgattg
    27001 agggcgcccc gttccttgaa cgaccccgtc atttggagat tttcgagctt gagatagatc
    27061 gtattgccgg taagccggga caaggtcctg gaatggacca ggggagattc gtagatggaa
    27121 gatgcgatgc gcgaggccgc ggctgctatc gactgcactg tcaccatgcc ctgctctcct
    27181 ttcactgcct caactgattg cgatcacaaa aaagcccctg acgggagtga atccgccagg
    27241 ggcctctgct gcataggtac acgtccggtg gcaggcaggc tcaggcggag ctggcgatca
    27301 tcatcgccgc cgccatgcga ataccaatgc cctgccgatt gatggtcatg cttgatccgt
    27361 cacgtcccga gagacactcg atgagcggag catataccag agccgggcta ggatcaagca
    27421 caccgtgaat tcaggaagcg tcctccccga tgacgccagc cacccgactc agaccgggac
    27481 catccactga gcaaccgact gtgtcgttct gcgacaggat gagccagtga tcgtggacga
    27541 gcctcgcgag aaaaagtaga atggcctcgt ccaactgatt cccgcgatat ccaatcactg
    27601 ccgcctgctg atcatgccgt atcgaaggca gtgatcgtat gtcttcatct ctcgtggaga
    27661 gcaccatgca aaagacccgc atcatctgca cgatcggacc tgcgaccgat tcctatgaga
    27721 tgctgcagaa gctctatgac gccggcatga gtatcgcccg tctcaatatg tcccacggcg
    27781 accacgaatc gcacgctaag gtcatccagc acatcaagag cctgaacagg aaagtgaagt
    27841 ttcccatccc cattcttctg gatacgcaag gcccggagat ccgcaccgga gatctctcga
    27901 atgaattgaa tctgcggcag ggcgaaatcg tcagcgtgac gactcgcggg ccgttgaacg
    27961 tcgaggaaag ctcaatccac atcaactatg ccgacttgct cgacgcggtc aacgtcgggg
    28021 accggatcac cgtcgacaac gggctgatta atttcgaggt cctggagaag catgaacgtc
    28081 atatgcgctg ccgcgtactc gacggagggc tgctgaagag caaacgccac gtgaatctcc
    28141 cgggcgtgcg cgtcaacctt ccggcgatca caccaaaaga cacgaaggat attctcttcg
    28201 gcctggaacg ggatgtggac ttcatcgcgc tttccttcgt gcgggaggcc ggagatatcc
    28261 agcaactgaa agcgctcatg ggcgacaagg tcggccgggt aaaaatcatc gccaagatcg
    28321 aggatcagga aggagtcaag aatctcgaat ccatcatcaa ggaatcggac ggcatcatgg
    28381 tcgcccgcgg agatctgggc gtggagatca atctggaaga tttgcccaat gtgcaacgca
    28441 cgatcgtgcg actctgcgcc gagtacggca agcgcgtgat tgtggccacg catctcctcg
    28501 aatccatgat tcaccatccg catcccactc gcgccgaagt caccgatgtg gccaatgcga
    28561 tctacgaaga ggccgatgcc gtcatgctct ccggcgaaac gaccgtggga aaatacccgg
    28621 tgaagtgcgt cgagtacctc cgcaggattg cgctcaagtc tgaaacgatc ccgggattac
    28681 agtttgccaa acgcttgcgc gatgtcggca acaaacagca gctggccgca gcggctgtgc
    28741 aactggcaga gggtgtcaag gccaaaggca tcgtcgtgat taccaggcgc gggatcatgg
    28801 ccgatctcgt cgccaactgc cgcccgtttg tcacgaacat ctatgcgttc acgaacatga
    28861 gccagccccg gcggacgatg ttgctgaatc gcggcgtctt tcccttcaaa atcgacttca
    28921 gttcagagcc tgagaaaacg ctccaaacgg cgttccggat cctcaaggat cgtgaacgat
    28981 tccaggccgg agacaaggtc gtcattatct cggacgtcct ggcgcagcaa cgggtggact
    29041 cgattcagat tcgcgacgta ccgtcggacg accttccggt ggaagcgtga tacgttagtc
    29101 ggggatttcc gcctcgacca gtttcgtgag cagggtcagc gattcctgcc agccaagata
    29161 acaggcttcc accggaatca tctccggaat cccctcctgc acggcggtca tctcggtgcc
    29221 gcacgacatt tgcttcaagg taatcgtcac gtgcatctca ccaggcagat tcggatcgtc
    29281 gaacacatcc gtgtagcgga ttcgctcgta cggcgtgagt tccagatatt tcccgccgaa
    29341 ggagtggctc tttcccgtgg tgaagttcgt gaacgacatc ttgaaggttc cgcccacctt
    29401 ggcgtccata tgatgcacgt ttccggtgaa cccgtttggt ggcagccatt tggccatggc
    29461 gtccgcatca agaaaggcac ggtatattcg ttccggcgtc gagcgaagta ctcggtgaag
    29521 acggacggta ttggtcggca tggtccgggc tcctttcaaa ggaagagcct ctcggtcggc
    29581 gagaagcatg attagcgatc gcgctatgca ggatgccagg agtcacgcat ttttgtctag
    29641 acgagatctc ggcataagca cgcatggaac aagcgcccgt ggtctgttac agtaaacgac
    29701 aaggcatcag ctcgcttcac ggtcatcgag aggaagcaga gcaacgggga tgacgatacc
    29761 gggcagctgg gctatcgcta acatggaccg cctgcattga aaaggaaggc atcatgaacg
    29821 ttcggcatcg ttcaatcact cacgcatgtg cgtcgatcac tctgacctgt ggtctcgtcc
    29881 tcgtgagcct caccggctcc ggctgtcaga ccgcgtatta cgaaacgatg gaaaagctgg
    29941 gctatcacaa acgtgacctg atggtcagtg atgtgaaaaa agcccgcgat gcccagcagg
    30001 acgcgaagga acaattcaaa tcggcgctcg atcgcttcac gaaaacgttg aatatccaag
    30061 gcggtgagtt gcaggacaag tacgaggtgt tgaacagcga ataccagcag agcgaaaaac
    30121 gcgcggaagc cgttcgcagc cgcatcgctt cagtggagga tgtgtcggac gccctcttca
    30181 cggagtggga gggggaactg aagcagtatt cgaacgcatc gctgcgtcag aaaagccaga
    30241 agcagctcgc gcagacgcgc acccagtacg ggcaattgat caaggccatg aaacgcgcgg
    30301 aggccaagat ggatcccgtg ttggccaaac tcaaggacca tgtcctgttc ttgaagcaca
    30361 acctcaatgc gcaggccatc gcctcgctga agagtgaatt gaccacggtc gaaggcaaca
    30421 tcgacttgct gatcaaggat ctcaacgcgt cgatccacga agcggatacc tttattgcct
    30481 ccatggaaaa agagaacgcc tgatcgcgag ccacccaccg gccgcacatt ttcgatcatt
    30541 cctggcagcc aatgagatct gcgcggccgg cggttcagca atgaaccagc taggcaagcg
    30601 cggggtcaga ctgggtcggc ttacgacgac gcctggcagc agcgagccgc gctccaagga
    30661 ggctcatgcc aaaggccgtg cccacgaaaa caggccacac gagagatttc atccagcctg
    30721 agctcgacat ggtcgccggc cacagccagg ccacccaggg taacgccgat cccacaagag
    30781 aacaggccgc atgaaccgga ggaagccgga aaaaccacgc gagaagcgcg cccgagagcg
    30841 catatgcggg aggcaccagc agcaggagaa agaacacgcc gttgaacatg gctgcgaaac
    30901 tgaggaaaaa ccataggaag ccgacgagaa acccggcaaa gaggccgcct cccgttcctc
    30961 tgctccgctg atgttgttga tccatggtcc cagtgtatcc cgtgaccacc agcgcaccaa
    31021 ggaattttag gttcggcgtc tacttagctg cggatgccgc ctgagtatct cgcccagccc
    31081 gcgccttcga tgcttcaagc tccggcaacg gctgcccgac gagaccctgg agatacccct
    31141 tatcgaccgc gtcgaacaga gcctggcgcg ctgccccctc cggtccttcg acggcgatca
    31201 ccacattgtg cacggcgagg cctccgctac ggcggg
//
LOCUS       DAOQEW010000020        30600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_322_length_30600_cov_8.086397, whole genome shotgun sequence.
ACCESSION   DAOQEW010000020 DAOQEW010000000
VERSION     DAOQEW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30600
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_30600_cov_8.086397"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLS35_03895"
     CDS             complement(<1..844)
                     /locus_tag="PLS35_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNI17829.1"
                     /translation="MQPKAPLPSGDATLKSGNAEQVKRISGQILAAADVDQILLDLRH
                     EILGCFDAEDLTLFVVDSEKKEIFSKIPHLDTVQEVRTPITEQSLAGFCAKYLRPVNV
                     GDAYSLVELSAIHPSLTHDATYDKSTGFKTKQVLAYPVVAENKYLMGVIQLLNKKSGG
                     RFTRKDEECVAEIAKSLGTAFYTLRKTSKKPPSKYDYLLTNGKISQHDLDVALAEAKK
                     GTTDIESLLIEKYKIPKAEIGKSLANFHKCPYIEYNERTIVDIELLKNLNVDYLKKNH
                     WMPLK"
     gene            complement(1083..2789)
                     /locus_tag="PLS35_03900"
     CDS             complement(1083..2789)
                     /locus_tag="PLS35_03900"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNI17830.1"
                     /translation="MRVSETLIPTLREDPGEAETVSHRLMLRAGLIRKVAAGIYTYLP
                     LGLRVLRKIERIVREEMNRAGAQEVLMPVASPAELWRETGRWDFYGKELLRFKDRHER
                     DFCLGPTHEEVITDLFRREVRSYRQMPLNFYQIQTKFRDEIRPRFGLMRGREFIMKDA
                     YSFDRDEAGARLNYQKMYDAYNRIFARCGLTFRPVEADTGLIGGSSSHEFMVLAETGE
                     ETIVYAEGGTYAANVERAEVLPPDVSPLPALRPLTAVSTPGRRTVEEVTKFLTIAPSQ
                     LVKTLLYTTGKETVAVLVRGDHEVNEIKLKRLLGATDIELVAPELIPKLTGAPVGFAG
                     PVGLKQIRILADWSVKAMANFVVGGNQVDTHFTDVNWERDFTVAQFSDLRNAQAGDLS
                     PRKDGVLKTAKGIEVGHVFMLGTKYSQAMKATFLDAQGQEQLAVMGCYGIGVSRVSAA
                     SVEQNHDAKGIKWPIPIAPFHVTLLPLSQSEPVLQLAESLYRAMEQAGIEVLWDDRDE
                     RAGVKFNDADLIGAPFHLVIGEKGLAQGQVELKLRHTGETKKIAPDQVIPTLTALLQA
                     AS"
     gene            complement(2796..4187)
                     /gene="rseP"
                     /locus_tag="PLS35_03905"
     CDS             complement(2796..4187)
                     /gene="rseP"
                     /locus_tag="PLS35_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNI17831.1"
                     /translation="MGTTFAWSPDVVSMLTHWALPFLVVLGVLVAFHEAGHFLAARWV
                     GVKVLKFSLGFGPKIFGRQIGETEYLLSIVPLGGYVKLFGEDEHEPLTPEDKKRAFVH
                     QSLWGKTLIVAAGPIFNFILAYLIYTAYIGLGYTLPVPSFKDIIPEIEAVLPGSPADQ
                     AGLKPGDRVIRVNEKEISTNAELLKYIAQSNGKQLTLDLTRGQQIKTVLVTPNKTTVQ
                     DNGKATTLFQLGIEERAPVITAVIPGSRAQAAGLVAGDRVLRIDGHDIFTWSQMTALV
                     RENPNHALQFEIQRNGSAQSVSVTPMGEKATVDGKPAEIGKIGISAQNQTILQTNEPL
                     KAPLLGAQATWGWTELTVVGIYKIITGDISRKNIGGPLTIAKTAGDAAEQGTSSLVFL
                     MAMLSINLGVLNLLPIPILDGGHLLFFFIEAIRRKPLEDRQRELAQQVGLVLLVGIMI
                     FAFWNDIERLISP"
     gene            complement(4213..5373)
                     /locus_tag="PLS35_03910"
     CDS             complement(4213..5373)
                     /locus_tag="PLS35_03910"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNI17832.1"
                     /translation="MKNIVILGSTGSIGTNTLDIVERFPQEFRVIGLTAGSNDEKLEA
                     QIRRFRPAFAALASEPAAAKLRQRCADLPVKILSGNEGVAQVAQSPDAELVISAIVGG
                     AGLLPTLAAIKAGKQIALANKEPMVMAGALMQAEAHKHQVRIFPIDSEHSAIFQSLEG
                     HRREDVKRIILTASGGPLWGFSREQLQDVSPERALQHPNWKMGSKITIDSATLMNKGL
                     EVIEARWLFDIPETQIEVLVHRESIIHSLVEYRDRSMIAQLGLPDMRTPISYAMRYPE
                     RMPLDLPSLDLTEVSTLTFFKPDHDRFPCLQLGYDALRIGGSMPATMNAANEVAVEAF
                     LQNGIRFLDIPDIIRSTMDAHQPRPIDGLDDALEADRWAREKAESLVHALTR"
     gene            complement(5406..6254)
                     /locus_tag="PLS35_03915"
     CDS             complement(5406..6254)
                     /locus_tag="PLS35_03915"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNI17833.1"
                     /translation="MSPTVDKARPRSSSETVRRVMAAVVFLPIFYILVHDLGPIAFFG
                     LVALSGMLAVAEFYRLHLAQAPWPWWSWAGVVATGLLLSSIQWPTIVADRTVLIATVV
                     MALCMPLLSGKALRDSLNDGMVLVMGVLYIGLLLGYLLLIRAMPDGALLIFFVVLVTW
                     AADTGAYIAGKSVGRHPLAPVVSPKKTYEGLAGGILLACLGAVAARAWFLPSISLVDC
                     LVLGVILTLAGLIGDLAESAMKRSTGFKDSGALIPGHGGMLDRLDSLLFTGPAFYYYV
                     AIVTTV"
     gene            complement(6295..7080)
                     /locus_tag="PLS35_03920"
     CDS             complement(6295..7080)
                     /locus_tag="PLS35_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNI17834.1"
                     /translation="MSDPRSRDIEPAPDSDLLSQLDPDLLPKHLAVIMDGNGRWAELR
                     GLPRIAGHQEGIKSVRELISLSLDLGIKVLTIYAFSQENWNRPAQEITALMGLLEHYL
                     STERSSLVEKGVRFQTIGRVTALPPSALRWVRTTEQETAHLDKLILNVALSYGGRAEL
                     ADAAKELARSVQEGRLSLDQIDERAMQQALYTHDLPDPDLLIRTSGETRISNFLLWQL
                     AYTELYFTPTLWPDFRRRETLVALIEYQRRERRFGRVFSSVSS"
     gene            7273..8715
                     /gene="tldD"
                     /locus_tag="PLS35_03925"
     CDS             7273..8715
                     /gene="tldD"
                     /locus_tag="PLS35_03925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNI17835.1"
                     /translation="MTNNETGALARFGVTDRDIEQALGRVKVSAVDYADLYFEYCQSE
                     SVSMEEGIVKRATKSVGQGVGVRATAGEKTGFAYSDELTAKDLNIAADTARYIANSVQ
                     GTAPVPVTHRPPLARNLYPHDRATIEVATRDRVTLLNAIDAEARRYDPRIKNVMAAYN
                     TEYKVMLVATSDGLMVGDIQPLSRLQVTCIAEENGNRQVGTFGGGGRIGLEYFQNDQR
                     YLHFAREAAREAILNLSAVDAPAGVMPVVLGGGWPGILLHEAIGHGLEADFNRKKTSA
                     FSTLMGKKVASEVCTIVDDGTLPFRRGSLNVDDEGTPTSRTVLIEKGILRGYITDKLN
                     ARLMGIPVTGNGRRESYQSVVLPRMTNTFMLAGESDPQDIIKSVKKGLYAVSFGGGQV
                     DITNGKFVFSASEAYLIEDGKITRPVKGATLIGSGPDILKQVSMVGHDLKLDEGIGTC
                     GKEGQSVPVGVGLPTIRIDEITVGGTKASS"
     gene            8712..10070
                     /locus_tag="PLS35_03930"
     CDS             8712..10070
                     /locus_tag="PLS35_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNI17836.1"
                     /translation="MTRMIGEQDYTTVAQDLLTRASKQGATAADVMVADGETLSVQVR
                     MGAVDRLTKAREKRLGLRVFFGQRSASASTSDFSRDSLERFVQETCALAQAVVEDPVS
                     GLPEPGQFATELPELHIHDSTKLQTDEQIALALRAERAAFAADPRITNSEGGECDSSS
                     GRIILANSHGFLGHYANSSFSLSVSPIASDAAGMQRDYWYGVNRSFAKLESPEAIGQE
                     ATRRTVRKLGARKAATGRVSVIFDPEVAGSLLSHLCSALSGYALYKGASFLIGQLGQQ
                     IAPEFVTIYDDGRMPGGLGTRPFDGEGLPTRKQAVVERGRLTSYLLDTYSGKKLGLPS
                     TGNAARSIGESPSAGPTNFYMVPGTTRPEDILASVKQGLYVTDLIGFGINMVTGDYSR
                     GASGFWIENGELAYPVEEITIASNLKQMYSAIETIGSDLVFRGRIASPTVKIAEMMVA
                     GN"
     gene            10091..10645
                     /locus_tag="PLS35_03935"
     CDS             10091..10645
                     /locus_tag="PLS35_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNI17837.1"
                     /translation="MQRLMASMLGALLTIPAIGMAAEFHVTSPTIKDQSTIGSEHVFN
                     GFGCTGQNVSPALQWEHAPKDTKSFAVTVYDPDAPTGSGWWHWLIFNLPPGTTGLSAG
                     AGNPDSAGAPAGSIQSMTDFGQPGFGGPCPPPGDKPHRYIFTVFALKVDQLPLKKDAS
                     GAMVGYYLNQNALGKASVTGLYGR"
     gene            10681..11136
                     /locus_tag="PLS35_03940"
     CDS             10681..11136
                     /locus_tag="PLS35_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17838.1"
                     /translation="MALDAEQGKIMLQLITSRYEDRHWRKKIEKTLSLPQSGVGDERQ
                     QQMFLYLKLNLKAYKSRRADPDDWILGGYATKEVIDRAKFQPHLVGQGITAEDVAFLG
                     VDPGPDVDEAWWEDMLVAWFGAPEDEEATEATEEESPSETAAESHPART"
     gene            complement(11196..11867)
                     /locus_tag="PLS35_03945"
     CDS             complement(11196..11867)
                     /locus_tag="PLS35_03945"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNI17839.1"
                     /translation="MSSPHIALQELADSLHDCQRCPLAKLGRRQVVFGVGNPQASVMF
                     VGEAPGFHEDQKGEPFVGAAGQLLNELLQSVGLSRADIYIANVIKCRPPNNRDPEPPE
                     VDTCKPFLLKQIALIRPKLVCSLGNWATQTLLERKVGITRVRGQAFYMKDFVLFPLLH
                     PAAALHQSSMLQPLREDFQKLKEFLDRHNQPAPAKEQEVASAARTLEIERPSSPAQQM
                     DLFGS"
     gene            complement(11962..13260)
                     /locus_tag="PLS35_03950"
     CDS             complement(11962..13260)
                     /locus_tag="PLS35_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI17840.1"
                     /translation="MLILGGGIAGLTAARSLAAKGHAVTVIEQGAHLGGRMWHAPPPI
                     LLEAHGATWSLLKTLGQDAVTRPLRHTPLEFLQSTGARIQFLHLPLPSPLNTLLGTTL
                     FQGLSMRDRWHLLSFLERTWEQDPPLPNDLDSRVADEWLTSIGQSETARHGVWNSLAR
                     LLLGAALEDISAGLFMRTLRRCFLTGARATKLIVPPQGVDSFLLAPLTRELDRLGVRV
                     KLNATATQVRFSHNRVTEVELADRTRLTADWYLSALPPQRLTPLLPERVVTHYAYFQQ
                     LSRLSESPLVMVRLHLDQPVRQTQLILLEGKTFHWIIRHPDEERHEQTSVLWAQAVGE
                     PSLLPQSTEELVRLAADAMAAAFPTTSPPRILDFDVMRLASAMLASKPGTHQYRPIST
                     SPFTNFFVAGAWTDTGWPANLESAILSGHRCAEAISTRPS"
     gene            complement(13269..14144)
                     /gene="hpnD"
                     /locus_tag="PLS35_03955"
     CDS             complement(13269..14144)
                     /gene="hpnD"
                     /locus_tag="PLS35_03955"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNI17841.1"
                     /translation="MMTPTEAQTYCTTLTKKSGSNFYYSFLFLPKARRDAMYTVYAFC
                     KEVDNAVDEPPPGSHPQEELARWRRELSAAYEGTPTLPVTISLARHVRELSIPQAYFE
                     ELIKGVEMDLTTTRYATFEQLSLYCYRVASVVGLICLHVFGTTSPRAQDYAVNLGMAF
                     QLTNILRDLGNDAECGRVYLPQEDFTRFGYREDDLLHRRYKPEFTELMIYEVGRAKEF
                     YAKAARALESLPREERQALTVAEIMRGVYSRILQRIEQSGYRVLGDRVTLTPSHRLAV
                     AAGVWLRSRLLSSAP"
     gene            complement(14141..15082)
                     /locus_tag="PLS35_03960"
     CDS             complement(14141..15082)
                     /locus_tag="PLS35_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNI17842.1"
                     /translation="MRICSLVPGATEVVAALGLADQLVGISHECDFPPQLSHAPVMVR
                     ARIESRSLASEQIDAQVGTMLSAGEGLYELDERGLLAAKPEVLITQNLCDVCAITPTQ
                     LTGILRKLAPAPRLVTLNPRRLDDILLDIATLGNALGQAEPATRFLSQLRGRLDAVHA
                     RIEKESLRPRVACLEWLSPLYTAGHWVPDMVAAAGGIDALAEAGAASHKIDWDVFAAS
                     EPDVIVLMPCGFTVARTKMELASLTSRPEWKDLPAVRRGQVYLVDALSYFSRPGPRLI
                     DGVEQLAAIFHPACFNHGLPEAVEQLTSDDIPTSMTR"
     gene            complement(15079..15786)
                     /locus_tag="PLS35_03965"
     CDS             complement(15079..15786)
                     /locus_tag="PLS35_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNI17843.1"
                     /translation="MSELRALIFDFDGVIADTEPLHFAALRQVLAGIDISLTEQEYYT
                     DYLGFDDRGCFLAALQSHQRQALPTLLGELMDRKAQAYLSAVREHLAIFPGVRELVHD
                     AAARYPLAIASGALRNEIELILEEAGLRKAFLHITSAEDVTRGKPAPEPFLHAMAALN
                     RQPSQPALTPDDCLVIEDSLPGIRAARAAGMKVLAVANTHTVQDLGEADAITHSLADT
                     RLSDLRSRLWGPAQGRL"
     gene            complement(15942..16709)
                     /locus_tag="PLS35_03970"
     CDS             complement(15942..16709)
                     /locus_tag="PLS35_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNI17844.1"
                     /translation="MVSSTGIPAIGSVWTDWRLLFASQPEPDLEFTEDDLDEAVPPPA
                     PPMNAPKRSKKGPFLWILLLLLVGGIGYVAMDPDAAMQMIQPYLDGGTETSQPVAQRP
                     PAPAPKPAPPAVQAPAAPIPAPAPAPSMAPPAVVDSVPPPPAEAPTPMPAPAPAAVVP
                     APVPAMKPASAPVRVAGPLYAEGQRVMVIADPSRPKSPMPLFADSIGTKTSTTVPAST
                     TLTILDGEYQKNGWIYSVRTQDGKKGWISERSLRLKR"
     gene            16993..17406
                     /locus_tag="PLS35_03975"
     CDS             16993..17406
                     /locus_tag="PLS35_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNI17845.1"
                     /translation="MRSYREELWFETKTRRAYLNITAQVEAAVRKSGVREGLVLVNAM
                     HITASVYINDDEAGLLQDYDTFLERLAPHDATYRHNNTGEDNGDAHIKRQLMGREVVV
                     AITDGKLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            17560..18573
                     /locus_tag="PLS35_03980"
     CDS             17560..18573
                     /locus_tag="PLS35_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17846.1"
                     /translation="MLSWSRPDPLTRDLIHLINARRHDHGDTDAAIDTLQAFIEQGRE
                     QDLLTVLAALDEEMAEWMFDLVAEASCAVIMDGPDEEKNAYALMAALELPLQANLGQP
                     LGLKIDPVATADLLHRHLHIPAGVVVRVEPRLLTDATLEGLTLQTLNEHLASIADSQR
                     TQAIASRLYPPVENTAFLFFQLIGMPDPGLPDSLEEPERRALLEGLVTQCSELALAPD
                     TLEVPVYASYALFDAQNAVRLHRLADETAAVWRDLDPMVRARTIVHLHTQCGNGVYMP
                     VWTDLFDPELPEDHGPVWTSPDVWVFTADLPIEWPGEMLTNRLLAEGCTRFENHIVEA
                     PEN"
     gene            18626..19234
                     /locus_tag="PLS35_03985"
     CDS             18626..19234
                     /locus_tag="PLS35_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNI17847.1"
                     /translation="MAGSSRIGAVVLVTLLSCLVLGGIWGVVLFVLPQSESPPEALPV
                     SVPTPPLPVPAATQPPDRPMIDDAARPGVANAEPPVVVPQPSVPPVAEAARMPIGREV
                     KCALEMDALCPEEEGDRRACLQRKAAQMSQPCRPMLRDKLARMKENWQQLRVACEADR
                     RQFCREVPSAGGELLLQCLESHAQEVSDQCFQFLPKRGRLLN"
     gene            complement(19235..19957)
                     /locus_tag="PLS35_03990"
     CDS             complement(19235..19957)
                     /locus_tag="PLS35_03990"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNI17848.1"
                     /translation="MSQHSIPPLLLVRSPHGGTRECEIRRTPFTIGRKPDNDLCLEDP
                     AVSAHHARIVQVQGVLFLEDLSSTNGSFVNEHPIDRRQLQDADALRFGTHRVIFREKP
                     GLAAEPIIPADMLVADKTMVVPSQAPRESPAPARKVGIVEILSGRTGQSHYPLTRQVS
                     LIGAQDDAVITLTGWFAPKTAAMISRRGEAYVVSQTESGKRILVNGRPVQREHALRHG
                     DVVEVAGITMRFVLRDERSHAA"
     gene            complement(19979..20803)
                     /locus_tag="PLS35_03995"
     CDS             complement(19979..20803)
                     /locus_tag="PLS35_03995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNI17849.1"
                     /translation="MHAWHVTHGAASDVGLVRRLNEDRYHADPAAGIFVVCDGMGGHR
                     AGEIASSRTVETIAHHLAEAETDRSLPLIGISRPEFSSATNRLASAVRLANHRVHQEA
                     ARRTEYAGMGTTVVAVRLTGAVLSIAHVGDSRLYLIRDGAVQLLTVDHSLVQEQMQSG
                     LLAAADMARAPHRHVLTRAVGVHPLVDVELGEMPVLPDDILLLCSDGLTAGVTADVIL
                     RVSQESIDPQAMADRLIALSNAAGGTDNATVIVALVTQRPTSLWDRIYTQLVSPPA"
     gene            complement(20812..22221)
                     /locus_tag="PLS35_04000"
     CDS             complement(20812..22221)
                     /locus_tag="PLS35_04000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNI17850.1"
                     /translation="MTTSWGWIGPYFIVILLAVLVGPFLATLPLFTHTYLRPLGMNVA
                     QVVRFIADGICLLMIWSAASRARRDLKDNGKGQTFLRAILFPSASLLIVIAAFQAYEA
                     HGISVLGPPRQPLYTWLVTGGLIGSATWLTFAWVRHVDALTSMFAWRPRRRAVAEEPV
                     RAEKPDEVRAADEAIPAAPPRPTAGIAVRNGNGVPSTLGRYQVLKELGRGAMGVVYLG
                     KDPTIQRFVAIKTMRLDDIDKEEDLKEFRDRFFREAESTGRLSHPNIITVYDAGEQDG
                     LAYIAMEYLEGVLLNCYCQKSTLVPAKQALQIVATVAEALDYAHSQGVIHRDVKPPNI
                     MILKQRLVKVMDFGIAKMASASKTQSSMIVGTPRYMSPEQATGKDVDGRSDVFSLGIV
                     LFELLTGERPFDAENMPALVMRIAKSPHAPLMKYRRDLPTRVQSILDRALQKEIQNRY
                     RHAGDMAQDLRDVFQVMPR"
     gene            complement(22362..23210)
                     /locus_tag="PLS35_04005"
     CDS             complement(22362..23210)
                     /locus_tag="PLS35_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNI17851.1"
                     /translation="MSRIDLHLHTTHSDGSCSTAEVMAFAKQAGLTALAITDHDIVDG
                     IPEATAIGKELGIEVVPGVEISSRLGESELHILGYFLNWTDPLLAQRLSTLRDSRHLR
                     NPKIVQRLNELGIPITYEEVRALAGTESVGRPHIARLLMEKKFVTSAKEAFDRYLANG
                     RPAFVDRELPEPAEAVRWIREAGGVPVLAHPTWVRTSAEGLRVLVRDLKAAGLGGMEV
                     HYSTHTPSQTTEYLDLARQCDLLVTGGSDFHGVTKPDIEVGIGRGQLKVSQKLLDPLR
                     KAATSS"
     gene            complement(23207..24145)
                     /gene="folB"
                     /locus_tag="PLS35_04010"
     CDS             complement(23207..24145)
                     /gene="folB"
                     /locus_tag="PLS35_04010"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNI17852.1"
                     /translation="MAERIVIERLEFYGRCGVTEEERRKPQLIVVDLELDAAVEAAAV
                     SDRLSETIDYAQVAERIVALSTSLTCQLLETLAEQLVGMLFAEFPADRVRIWIRKVHA
                     PLAMVAGSVGIRFERTRAAHQSTHQALSAAPFLIQQLARLPKGHILDVAAGRGRNALY
                     LLAQGSQVEAIDRDTDALSALEAAAGRQRLSGLTTRVLDLEASADHPPSLGHECYDAI
                     VVFFYLHRPLFPVIIDALKPNGILLYETFTIDNYFRHQHPRRWEFCLAQNELLRLTSP
                     LRVLHYDEGEHDGGHGSGPCYTARLVAQKTGPETSP"
     gene            complement(24204..24674)
                     /locus_tag="PLS35_04015"
     CDS             complement(24204..24674)
                     /locus_tag="PLS35_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17853.1"
                     /translation="MFLPARVFTSKNRRTSLQSIPRRFNGQSIDEQIRAVQSAWADVL
                     TLPLCLTVLAFYEWWRWLFSIPSNPLLLTVVAAVAIHATRRRRKAYKAELKQLHLNRT
                     PQPTVSDVTEFLYRGARRMVQDFTAKCSPATIVASARRFASPAHWLTKRLFARS"
     gene            complement(24805..25044)
                     /locus_tag="PLS35_04020"
     CDS             complement(24805..25044)
                     /locus_tag="PLS35_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17854.1"
                     /translation="MIDKPTLSFSESLTPERRTAFYEGHKPIAVVMIFIVFLLPFAGL
                     LVYGLIGAAASVLISALGYYLTPYLTQRLSLLAGR"
     gene            complement(25163..25681)
                     /locus_tag="PLS35_04025"
     CDS             complement(25163..25681)
                     /locus_tag="PLS35_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17855.1"
                     /translation="MTMRHFVHIAAIIGVLAVSGSTEAAELAGAWKGVLSSPDGSRAE
                     VQIDFSPQGLPLYSYTNNKGVTRQVELSHVGQTIEYVPPGGGVQRMAVKRFEQGPGRV
                     SISIIGSFERASQGYMDQRQEAALFEYTLVPEGMKMRVTTRTASHFGDKDLIVGGDPS
                     SVVAEGVLHRLH"
     gene            complement(25726..26637)
                     /locus_tag="PLS35_04030"
     CDS             complement(25726..26637)
                     /locus_tag="PLS35_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI17856.1"
                     /translation="MAKTTTASAAKPKALSLHIGLNAVSPAAYGGWSGELAACEFDAK
                     DMAAIAKSQGMKSTVLLTKQGTRAKMLAAMRKAAKQLKQGDLFFLTYSGHGGQVPDVT
                     GEEIDKQDETWCLYDGQLIDDELYFELSRFAAGVRILVLSDSCHSGTVTRAVPPPIDR
                     TKVTGRSKMMPPAVAIRTYREHQAFYNKLQHDVAKAAGKAGTTDPDSVLAQLAVSPRL
                     TAIAKEFHPAVILISGCQDNQTSMDGDHNGAFTEQVLKAWNHGNYRGNYAKFHAAVKA
                     GLPASQSPNLFTLGAAARFLRQQPFKV"
     gene            complement(26816..26971)
                     /locus_tag="PLS35_04035"
     CDS             complement(26816..26971)
                     /locus_tag="PLS35_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17857.1"
                     /translation="MVVKQGYRLIVVDKDGVLVSEFQLTAQALAAPETFVAAIKESIE
                     EIEEEDT"
     gene            27161..28183
                     /locus_tag="PLS35_04040"
     CDS             27161..28183
                     /locus_tag="PLS35_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNI17858.1"
                     /translation="MHTTKLIAAFLVLVLVGVPTISFAKARGGSGGGFSSGSRSGSSG
                     SMGSRGSRTYQDNGAKPIEQSTTARPSATPPPSMAGNPSTASRPAPAAQPSWFQRNPL
                     LAGIVGGLAGTWLGHMIFGATDTSARTTEGGEGEQAAAGAGSSGMLLLLMVMGGGALW
                     WYMRSRRTPAPNFSGLSRTSVASGSLLAESSRAGFGAAAVEEEMSSTDKAAFQQALID
                     IQTAWSHQDLAGLRRLVTPEMLDYFSTGLAEQASEGVANRVEDVVLGQAEVRESWSEG
                     ATQYATVSLCWRAKDYTISLAKQPGEQGYIVEGNAELPIETTEVWTFMRFGAGKWLLS
                     AIQQVS"
     gene            complement(28385..28696)
                     /locus_tag="PLS35_04045"
     CDS             complement(28385..28696)
                     /locus_tag="PLS35_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17859.1"
                     /translation="MPISRRPELEKDNPVAPQIYVGPRRKRSLARKTSSELQAPSDEM
                     ATRLQRLVEAAEQFVHSMPPVQETDQERHALLEAISQAQGCLPIEDTAASRLRQASHT
                     R"
     gene            29463..29963
                     /gene="moaC"
                     /locus_tag="PLS35_04050"
     CDS             29463..29963
                     /gene="moaC"
                     /locus_tag="PLS35_04050"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNI17860.1"
                     /translation="MADFTHFNESGRARMVDVGAKASTERLATAQAIVFLQPETLEKI
                     QQGKIAKGDVLSVAQVAGVMGAKKTPDLIPMCHPILLTSVDISFQEIAQRDREGRCSI
                     VISATAKTTGPTGVEMEAMTAASVAALTIYDMCKAVDRGMSFGDICLLTKSGGKSGTY
                     QRPVPV"
     gene            29973..30236
                     /locus_tag="PLS35_04055"
     CDS             29973..30236
                     /locus_tag="PLS35_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNI17861.1"
                     /translation="MVTVRLFGMTKMLAGNQGALSLALPEGRRVKDLVSVIEAAYPKI
                     GELLHKKKVLVSVNQDIAHEDLEVRDGDEIALLPPFAGGSAGC"
     gene            30385..>30600
                     /locus_tag="PLS35_04060"
     CDS             30385..>30600
                     /locus_tag="PLS35_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNI17862.1"
                     /translation="MSLEQVTKADQPVTGDEAMLVRVQREDFSIDEELCRVRARSKRI
                     GGIAIFLGTARDRSKGKDVDGITFEHYE"
BASE COUNT         6186 a         8518 c         9516 g         6380 t
ORIGIN      
        1 gcttgagcgg catccagtga ttcttcttca ggtagtcgac gttgaggttc ttgagcaatt
       61 cgatgtcgac gatcgtgcgc tcgttgtatt cgatataggg gcacttgtgg aaattggcga
      121 gtgatttgcc gatctcagct ttcgggatct tgtatttctc aatgagcagc gactcgatat
      181 ccgtcgtgcc cttttttgcc tcagcgagtg cgacatcgag atcgtgttgg gaaatttttc
      241 cattggtgag caggtagtcg tatttggacg gcggcttttt cgaggtcttt cgaagggtgt
      301 agaacgcggt gcccagggat ttggcaatct ccgcgacgca ctcttcgtct ttgcgcgtga
      361 agcgtccgcc gctctttttg ttcaggagtt gaatcacccc catcagatac ttgttttcgg
      421 cgacgaccgg ataggcgaga acttgtttgg ttttgaagcc ggtactttta tcgtacgtgg
      481 catcgtgggt cagcgacgga tgaatcgcgc tcagttcgac caagctgtag gcatcaccga
      541 cattgaccgg gcgcaggtat ttggcgcaaa accccgccag gctttgctca gtgatcggcg
      601 tacgcacttc ctggacggta tcgagatgag gaatcttgga gaagatttct tttttctcgg
      661 aatccacgac gaagagggtg aggtcttctg cgtcgaaaca tccgaggatt tcgtggcgta
      721 gatccaggag aatttgatcg acgtctgcgg cggcgaggat ttgtccgctg atccgcttga
      781 cctgttcggc gttcccggat ttgagagtgg catcgccact aggcagcgga gctttaggct
      841 gcatcaacgt gtctccacgc gggctgaacg gggaattgca tgcagggtca gaaacgcgat
      901 gccggcctgg aatcgttcgc cgtacaggag tttcggacgg tgcgtcttcc gtgtgagtca
      961 gtgtatcgca acttgtagtc aaggcaagca aaaggcattt tctcaaaccc cggcctggag
     1021 cttgccttcg tcggcgtgat gagattgtcg tgaaggtggc gaacgaggat cgggagtgga
     1081 ttttaggacg cagcttggag cagtgccgtg agtgtcggaa tgacctgatc cggagcaatt
     1141 tttttcgttt cgccggtgtg acgaagtttg agctcgacct gcccctgcgc caagcctttt
     1201 tctccgatca ccaggtgaaa gggggcgccg atcaggtcgg catcgttgaa tttaacaccg
     1261 gctcgctcgt cgcgatcatc ccacaggact tcaatgccgg cttgttccat cgctcggtag
     1321 agcgactcgg cgagttggag aaccggctca gactggctga gcggcaacag cgtgacatga
     1381 aagggcgcga taggaatcgg ccacttgatg cccttggcgt cgtgattctg ttcgactgat
     1441 gcggccgaaa cacggctcac gccaatgcca taacaaccca tgacggccag ttgttcctgg
     1501 ccctgcgcat ccaggaaggt ggccttcatc gcctggctgt atttcgtgcc cagcatgaag
     1561 acgtggccga cttcgatacc cttcgccgtc ttgagcacgc catccttgcg cggggagaga
     1621 tcgccggctt gcgcattgcg gaggtccgag aattgggcga ctgtgaaatc gcgttcccag
     1681 ttcacgtcgg tgaaatgggt atcgacctgg ttgccgccga cgacaaagtt cgccatggcc
     1741 tttacggacc aatcggcgag gattcggatc tgtttcaagc cgactggccc tgcaaagccg
     1801 actggtgcac cggtcagctt gggtatcaac tccggcgcga cgagctcgat atcagttgcc
     1861 ccgagcaggc gcttgagctt gatttcgttc acctcatgat cgcctcgaac caggacggcg
     1921 acggtttcct tgccggtggt gtagagcagc gttttgacga gttgcgaggg agcaatggtt
     1981 aagaacttgg tgacctcttc taccgtccgt cgccccgggg tggagacggc ggtgaggggt
     2041 cgtaaggccg gcagcgggga tacgtcgggt ggaaggacct cggcccgttc gacgttggcg
     2101 gcgtaggtgc ctccctcggc gtagacgata gtttcctcac cggtttctgc cagcaccata
     2161 aactcgtggg aggaacttcc tccgatcaat ccggtgtctg cttccacggg acggaaggtc
     2221 aatccgcagc gagcgaagat gcggttatag gcgtcataca ttttctgata gttcagcctg
     2281 gctcccgctt cgtcccgatc aaagctgtac gcatccttca tgatgaactc gcggccgcgc
     2341 atcaagccga aacgcggacg gatttcgtca cggaacttcg tctggatctg atagaagttc
     2401 agtggcatct gccgatagga acggacttcg cggcggaaga gatcagtaat gacttcctcg
     2461 tgggtcgggc ccaggcagaa atcgcgctca tgccggtctt tgaaccggag gagctctttg
     2521 ccatagaagt cccaccggcc ggtttcacgc cacagttcgg ccggcgatgc gaccggcatg
     2581 aggacttcct gtgcgccggc ccggttcatt tcctcgcgga cgatgcgttc gattttccgt
     2641 agaacgcgca acccaagcgg aagataggta tagatgccgg cggcaacttt ccggatgagg
     2701 ccggcgcgca gcatgaggcg atgactgacc gtttcagcct cgcccggatc ctcacgtaat
     2761 gttggaatga gagtttcgga gacgcgcatg tggggctatg gggaaatcaa tcgctcgatg
     2821 tcattccaaa aggcgaaaat cataataccg accagaagta ccaaccccac ctgttgtgcc
     2881 agttctcgct gccgatcttc aagtggtttt cgtcgaatcg cttcaataaa aaagaataac
     2941 agatggccgc cgtcgagaat tgggatgggt agaagattga ggacacccag gttgatgctc
     3001 agcatcgcca tgaggaagac cagactggag gtgccctgtt cggccgcatc gccggcggtt
     3061 ttggcgatgg tgagcgggcc gccgatattc ttgcgggaaa tatctccggt gatgatcttg
     3121 taaattccga ccaccgtgag ttcggtccat ccccacgtcg cctgggcgcc caataacggg
     3181 gctttcaagg gttcgttggt ttgtaaaata gtctggttct gggcagagat gccgattttt
     3241 ccgatctcgg ccggctttcc gtctaccgtg gccttttcac ccatcggcgt tacggaaacc
     3301 gattgggcgc ttccgtttcg ttggatctca aactgcaagg cgtgattcgg attttcccgc
     3361 accagcgcgg tcatctgcga ccaggtgaag atgtcatgcc catcaatgcg aaggacacgg
     3421 tcgccggcta caagcccagc ggcttgtgcg cgcgatcccg ggatgacggc cgtaataacc
     3481 ggcgcgcgtt cctcaatccc gagctggaac agggtcgtgg ctttgccgtt gtcttggacg
     3541 gtggtcttat tgggggtgac gagcaccgtt ttgatctgct gcccgcgcgt caggtccaag
     3601 gtgagttgct tcccattgct ctgggcgatg tacttcagca gttccgcatt cgtcgaaatt
     3661 tctttctcat tcacccggat gacccggtca cctggtttca atcctgcctg atcggctggc
     3721 gaccccggga gcacggcttc aatttcagga atgatgtctt tgaagcttgg cacgggaagg
     3781 gtgtagccaa gcccgatgta ggccgtatag atgagatagg ccaggatgaa gttgaaaatc
     3841 ggtcccgccg ccacaatcag ggtcttcccc cagagagatt gatgaacgaa ggcccgcttt
     3901 ttgtcttccg gagtcagcgg ttcatgctcg tcttctccga acagcttgac gtagccgccg
     3961 agcggcacga tcgacaggag gtattcggtt tcaccgatct gtcgtccgaa gatcttgggc
     4021 ccgaacccga gtgaaaactt cagaaccttc acgccgaccc agcgtgccgc taggaaatgc
     4081 cccgcttcat ggaaggcgac gagtacgccc aggaccacca ggaacggaag ggcccagtga
     4141 gtcagcatcg acaccacgtc aggggaccag gcaaaggttg ttcccacaac aacactcctt
     4201 aggttcaggg tattaccgcg tcaaggcatg cacgagggac tcggcttttt cccgagccca
     4261 gcgatccgct tccagcgcat catccaaacc atcgatgggt ctcggctggt gagcgtccat
     4321 tgtactccga ataatgtccg gaatatcgag aaaacgaata ccgttctgca ggaaggcttc
     4381 caccgccact tcattggcgg cgttcatcgt ggccggcata ctgccgccga tccgcagggc
     4441 gtcgtagccc agttgcaggc acggaaagcg atcatgatcc ggcttgaaga aggtcaaggt
     4501 gctcacttcg gtcaaatcca gagagggaag atccaacggc atccgttcag gataccgcat
     4561 ggcgtaagaa ataggggttc gcatatcggg caggcccaat tgcgcgatca tcgaccgatc
     4621 tctgtattct accagagaat ggataatgct ctcccgatgc accaagacct cgatctgggt
     4681 ttccggaata tcaaagagcc aacgcgcctc aataacctcc aatcccttat tcattaaggt
     4741 tgccgaatcg atggtgattt tcgatcccat tttccagttc gggtgttgca acgctcgttc
     4801 agggctgacg tcttgcaact gctcccggga aaagccccag agcggtccgc cggatgccgt
     4861 gaggataatg cgtttgacgt cctcacgccg gtgcccttca agggattgga agatcgcgct
     4921 atgttcacta tcgatgggga aaatgcgaac ctgatgtttg tgggcttctg cctgcatcaa
     4981 ggcgccggcc atcaccatcg gttccttatt cgccagggcg atttgtttgc cggccttgat
     5041 tgcagccagc gtgggcagca atcccgcgcc gcccacgatc gctgaaatca ccaattcagc
     5101 gtccggggat tgtgcgacct gggccacgcc ttcgttgccg gagagaattt ttaccggcag
     5161 gtctgcgcac cgttgtcgca acttcgcggc ggccggttcg ctggccaggg cggcaaaggc
     5221 cggccggaaa cgccggattt gcgcttcaag tttctcgtca ttcgagccag cggtcagacc
     5281 gatgacgcga aactcttggg gaaaccgttc cacgatatcg agggtgttcg tgccgatcga
     5341 gccggtcgaa cccaaaatca cgatgttctt catggcatac tccagagcag gcgaccgatc
     5401 acccgttaca cggtcgtgac gatcgcgaca tagtagtaga aggccggtcc agtgaacaag
     5461 aggctatcca gacggtccag catcccgccg tggcctggaa tcagggcgcc ggaatccttg
     5521 aatcccgtgc tgcgtttcat ggccgattca gccaggtcgc cgatgaggcc ggcgagggtg
     5581 aggatcacgc caagcaccag acaatcgacg agtgagatgc tgggaaggaa ccaggcgcgt
     5641 gccgcgaccg cccccaggca ggccagaaga atgcctcccg ccaagccctc ataggttttt
     5701 ttcgggctga ccaccggggc caatgggtgt cgtccaacgg actttcctgc gatgtaggcg
     5761 ccggtatccg ctgcccaggt caccagcacg acaaagaaaa tcagtaaggc cccgtcaggc
     5821 atggctcgaa tgagcagcag gtagcccagc aaaagtccga tatacaggac gcccatcacc
     5881 agcaccatgc catcgttcag cgaatcgcgc agcgctttgc ctgagagcag gggcatacag
     5941 agcgccatca ccacggtcgc tatcaagaca gtacggtctg ccacgattgt cggccactgg
     6001 atgctgctca acagtaaccc cgttgcgacg actcctgccc agctccacca gggccaaggc
     6061 gcttgggcca aatgaagccg atagaattct gcgaccgcca gcatgccgga gagggcaacc
     6121 aatccgaaga aggcgatcgg cccgaggtcg tgcaccagga tatagaaaat cggcagaaaa
     6181 acaaccgcgg ccatcacccg gcgaacggtt tccgaggagc tgcgtggtcg agccttgtcc
     6241 accgtgggtg acatctcctc gcactggcat gctcccggtt tcaccacggg aagactagga
     6301 ggagacgcta ctgaacactc ggccgaaacg gcgctcgcgc cgctggtatt cgatcaacgc
     6361 gaccaaggtt tcgcgccgac ggaaatccgg ccagagggtc ggggtgaagt agagttccgt
     6421 ataggccagt tgccataaca gaaaattgct gatgcgggtt tcgccgctcg tgcggatcaa
     6481 cagatccggg tcgggcaggt cgtgcgtata cagggcctgt tgcatggcgc gttcatcgat
     6541 ctgatccaaa gacaggcgcc cttcctgcac agaccgcgca agctccttcg cggcatcggc
     6601 cagttccgcg cgcccgccgt agctgagcgc gacgttcagg attaacttgt ccagatgcgc
     6661 cgtttcctgt tcggtcgtgc gcacccagcg cagtgccgat ggcggcagcg cagtgacccg
     6721 gccgatggtc tggaagcgca cgcccttttc gaccaggctg gaacgctcgg tggagagata
     6781 gtgttccagc aatcccatca aggccgtgat ttcctgggcc ggccgattcc aattttcctg
     6841 ggaaaaggca tagatggtga gcaccttgat gccgaggtcg agcgacagcg agatcaattc
     6901 ccggacggac ttaatgcctt cctggtgacc ggcaatacgg ggtagtccac gcaattcggc
     6961 ccatcggcca ttgccatcca tgatgacggc caaatgtttc ggcagcagat cagggtcgag
     7021 ttgcgagagc aggtcggagt cgggtgcagg ctcgatatcg cgggatcgag gatcgctcat
     7081 ggaacggcgt aggcaccttt tcttgtgacg acgtgaatcc ttagacaggt aacgcgagtc
     7141 tagcggtggg gtgtgggaaa gtcaaactat atttcccaat ttcgctgtat ttgcaggtgg
     7201 ttcggaagtg tttacattgc gcctccagaa aagagtacgt tatactcacg caccgtatga
     7261 gaggagtaat ccatgaccaa taacgagacc ggcgcgctgg cgcgtttcgg ggtcaccgat
     7321 cgagatatcg agcaggcgct gggacgggtg aaggtctccg ctgtcgacta tgccgacctg
     7381 tatttcgaat attgccagtc ggaatcggtc tccatggaag aggggatcgt caaacgcgcg
     7441 acaaaaagtg tcgggcaggg tgttggggtg cgggcgacgg ccggtgaaaa aaccgggttt
     7501 gcctattccg atgaactcac cgccaaggat ttgaacattg ccgccgatac ggcacgctat
     7561 atcgccaatt ctgtgcaagg cacggccccg gttcccgtca cccatcggcc acctctggct
     7621 cgtaatctgt atcctcacga ccgcgctacg atcgaagtgg cgacccggga ccgggtgacg
     7681 ctgttgaacg ccatcgatgc ggaagccagg cgctatgatc cccggatcaa gaacgtcatg
     7741 gcggcctata acaccgaata taaagtgatg ttagtcgcga cgtccgacgg actcatggtc
     7801 ggagatattc agccgttgtc caggctacag gtgacctgta tcgcggaaga aaacggtaac
     7861 cggcaggtcg gtacattcgg cgggggcggc cggatcgggc tggaatattt tcaaaacgat
     7921 cagcggtatc tgcactttgc gcgggaagcg gcgcgggaag ccattctgaa tctgagcgcc
     7981 gtcgatgccc cggccggtgt catgccggtt gtgctgggcg gtggttggcc gggcattttg
     8041 ctccacgaag ccatcggcca cgggttggaa gcggatttca atcgtaagaa aacctcagcg
     8101 ttctcgacct tgatggggaa aaaggtggcg tccgaggtct gcaccatcgt cgatgacggt
     8161 acgcttccgt tccggcgcgg ttcgttgaat gtcgacgatg aagggacgcc aaccagccgt
     8221 acggtcttga tcgaaaaggg cattctacgt ggttatatca ccgacaaact gaatgccagg
     8281 ctcatgggca ttccggtgac cggcaacggg cggcgcgaaa gttatcagag tgtggtgttg
     8341 ccgcggatga cgaatacctt catgctggcc ggtgaatccg atccgcagga catcatcaaa
     8401 tcggtcaaga agggcctcta cgccgtgtca tttggcggcg ggcaggttga tatcaccaat
     8461 ggcaaattcg tgttttccgc cagcgaggcc tacctcatcg aggacgggaa aatcacccgt
     8521 cccgtgaagg gcgccacgtt gatcggcagc ggaccggaca ttttgaagca agtttccatg
     8581 gtgggccatg atttgaagct cgatgaagga attggcacct gtggtaagga agggcagtca
     8641 gtcccggttg gcgtcggcct acccaccatc cgcatcgacg aaatcaccgt cggtggaacg
     8701 aaagcatcat catgacacgt atgatcggag agcaagacta tacgacggtc gcgcaggatt
     8761 tgctgacgcg agcttcgaaa cagggcgcga cggcggcgga cgtcatggtg gccgacgggg
     8821 aaaccctctc ggtacaggtc cgaatggggg ccgtcgatcg cttgacgaaa gcgcgtgaga
     8881 agcgattggg gttgcgggtt ttctttggcc agcggtcagc cagtgcgtcc acctccgact
     8941 tttctcgcga ctcgctggaa cggtttgtcc aggaaacctg tgccttggct caagcggtgg
     9001 tggaggatcc tgtctccggc cttcccgaac cggggcagtt cgctaccgaa ttgcccgagt
     9061 tgcacatcca cgatagcacg aagctgcaga cggatgagca gattgccttg gcccttcgcg
     9121 ctgaacgcgc ggcgtttgcc gcggatcctc gcattacgaa ctcggaaggg ggcgaatgcg
     9181 actcgtcctc cggccggatc attttagcca acagccatgg gtttctcggg cactacgcca
     9241 acagcagttt ttccctgtcg gtctcgccga tcgcctcgga tgcggccggg atgcagcggg
     9301 attattggta tggcgtgaac cgatcgttcg ccaaactgga aagcccggaa gccatcggac
     9361 aagaggccac caggcgcacg gtccgcaagt tgggcgcccg caaggcggcg actggccggg
     9421 tgtcggtgat ctttgatccc gaagtggcgg gcagtctgct gagtcatctc tgcagtgcgc
     9481 tgtctggtta cgccctgtac aagggggcct catttctcat cgggcaactc ggccagcaga
     9541 tcgctccgga atttgtgacg atttatgacg acggacggat gcccggtggt ctgggcacgc
     9601 gcccgtttga cggcgaggga ttgccgacaa gaaaacaggc cgtggttgaa cgcggccggt
     9661 tgaccagtta tttgctggac acgtactccg ggaagaagtt gggattgcct tcgaccggaa
     9721 acgcagcgcg cagcatcggt gaaagtcctt ctgccggccc gacgaacttt tatatggtgc
     9781 cgggcacgac caggccggaa gacattctgg cgtcggtgaa acaaggattg tacgtgaccg
     9841 atctgatcgg cttcggcatc aacatggtca ccggagatta ttcccgcggg gcgagcgggt
     9901 tctggatcga gaacggcgag ttagcctatc cggtcgaaga aattaccatc gcgagcaatt
     9961 tgaagcagat gtattcagcc attgagacaa tcggctcgga tctggtgttc cgcggacgca
    10021 tcgccagccc gacggtgaag attgccgaaa tgatggtcgc agggaactag cgacaaaggg
    10081 gggatgggtg atgcaacgat tgatggcctc aatgctcggc gccctactga cgatccctgc
    10141 gatcggcatg gcggcagagt ttcacgtgac cagcccgacg atcaaggatc agagcacgat
    10201 cggtagtgaa cacgtgttca acggatttgg ctgtacgggg cagaatgtct cgcctgcgct
    10261 gcaatgggag catgccccga aggacacgaa gagttttgct gtgacggtgt acgatcccga
    10321 tgctcccacc ggaagcggct ggtggcattg gctcatcttc aaccttccgc cgggtaccac
    10381 cggtttgtcg gctggtgcag gaaatccgga cagcgccggc gctccggcgg gcagcattca
    10441 aagcatgacc gattttggac agccgggctt cggggggcca tgccctcccc cgggagacaa
    10501 gccgcatcgg tatatcttta cggtctttgc gttgaaggtg gaccaattgc cgctgaagaa
    10561 agacgcgtcc ggcgcgatgg tcgggtatta cctcaatcag aatgccctcg gaaaagcatc
    10621 ggtcaccggg ttatatggac gataagcagg caatcatcag aacagagtaa ggaacacacc
    10681 atggctcttg atgccgaaca gggaaaaatc atgctgcagc tgatcacctc gcgatacgag
    10741 gatcggcatt ggcgcaagaa gatcgagaag acgctcagtt tgccgcagag cggagtcggg
    10801 gacgagcgcc agcagcagat gtttctctat ctgaagctga atttgaaggc gtacaaatct
    10861 aggcgggcgg accccgacga ctggatctta ggcggatacg ccacgaagga agtcatcgat
    10921 cgggcgaagt ttcaaccgca cttggtgggg cagggaatca cggctgagga cgtcgcgttc
    10981 ctcggtgttg atccgggccc ggatgttgat gaagcctggt gggaagacat gctggttgcc
    11041 tggtttggtg ctcccgaaga tgaagaagcg actgaggcaa cggaggagga atctccgtcc
    11101 gaaaccgctg ccgagagtca tcctgcccgt acgtgaacga acgcgctgtc tggctgcttc
    11161 gcctgttggt gaggcggcca ggccaggcgg cgttgctaag atccgaataa atccatttgc
    11221 tgggcgggcg aagacggacg ctcgatctca agtgtccgtg cagcactggc aacttcttgt
    11281 tccttcgccg gggctggctg attgtgccgg tccaaaaact ccttgagctt ctgaaaatct
    11341 tcccgcaagg gctgcagcat gctgctttga tgcagggctg cggcaggatg aagcaaagga
    11401 aagagcacaa agtctttcat gtaaaaagcc tgcccgcgga ctcgcgtaat gcctacctta
    11461 cgttccagca gcgtttgtgt cgcccagttg ccgagcgagc agaccaactt cggtcgaatc
    11521 agagcgatct gtttcaggag gaacggtttg caggtgtcga cttccggcgg ttccgggtcg
    11581 cgattattgg gcggccgaca tttgatgacg ttggcaatgt agatatctgc gcgggatagt
    11641 ccgaccgact gcagcagttc attcagcaat tgccccgccg cgcctacgaa cggctcacct
    11701 ttttgatcct cgtgaaagcc cggtgcttcc cccacaaaca tgacggaggc ctgcggattg
    11761 cccacgccga ataccacctg acgccggcct agtttggcga gcgggcatcg ttggcagtcg
    11821 tgaagcgagt cggcgagttc ctgcagggcg atatgcggtg aagacatgga cgtgacatct
    11881 cgcgtggagc gccaaatctt agtcgcgcca tcggggcttg tcaacgctgc gctgcgcgtt
    11941 ggcgcgcacc ggtgcgagtc gttatgaggg acgggtagag atggcctcgg cacaacggtg
    12001 gccgctgaga atggctgatt ccagattggc cggccatccc gtgtccgtcc acgctcccgc
    12061 cacgaaaaaa ttggtaaagg ggctggtcga gatcggtcga tactgatgcg tgcccggttt
    12121 gctggccagc atggcggagg cgaggcgcat gacgtcgaag tcgagaatgc gcggcggcga
    12181 cgtcgtcgga aatgcggccg ccatggcatc cgcggcaagg cgaacgagtt cttccgtcga
    12241 ctgtggcaac agacttggct cgcccaccgc ctgagcccag aggaccgacg tctgctcgtg
    12301 tcgctcttca tcgggatgac ggatgatcca gtggaaagtt tttccttcga ggaggatgag
    12361 ctgtgtctgt cgaaccggct ggtcgaggtg caggcgaacc atcacgagcg gggattcgct
    12421 gagacggctg agttgctgga aataggcata gtgagtcacc acgcgttccg ggagaagcgg
    12481 tgtgagacgc tggggcggca gcgcggagag ataccagtcg gcagtgagcc tggtccgatc
    12541 ggctaactca acttctgtga ctcggttgtg tgagaagcgc acttgagtgg ctgtggcatt
    12601 cagtttcact cgaacgccga gccggtcgag ttctctcgtc aggggcgcca gcaaaaatga
    12661 atccactcct tgaggcggca cgatcagctt cgttgcccgg gcaccggtca agaaacaccg
    12721 ccgcaaggta cgcataaaca ggccggcaga aatgtcttcc agcgcggctc ccaacaacag
    12781 cctggcgaga ctgttccaca caccgtgacg ggcggtttcc gactggccga tgctcgtgag
    12841 ccattcgtcg gccacacgcg agtcgagatc gttcggcagc ggcgggtcct gttcccaggt
    12901 ccgctcgaga aacgagagca ggtgccagcg gtcacgcatg gacaggccct ggaataaggt
    12961 cgtcccgagc aacgtattta atggagaggg gagaggaaga tgcaggaact ggatgcgggc
    13021 gccggtcgat tgcaaaaatt ccaacggagt atgccggagc ggtcgggtca ctgcgtcctg
    13081 gccgagagtc ttgagcaatg accaggtggc tccatgcgct tcgaggagaa tgggtggcgg
    13141 cgcatgccac atccggcctc caaggtgagc gccttgctcg atcacggtga cggcgtggcc
    13201 tttggcggcg agggaacggg ccgcggtcag accggcgatg ccgccgccca ggatgagcac
    13261 cgatcggctc atggggcgga ggagagaagg cgggagcgca gccagactcc ggctgccacc
    13321 gctaaccggt ggctaggggt gagggtcacc cgatctccga gcacgcggta accggattgc
    13381 tcgatgcgct gcaaaatacg gctgtacaca ccgcgcatga tttcggctac ggtcagagcc
    13441 tggcgttctt cgcgtggcag tgactcgagc gcgcgcgcgg ctttggcata aaattccttc
    13501 gcgcgtccga cctcatagat catgagttcg gtgaactccg gcttataccg gcggtgcaga
    13561 agatcgtctt cccggtagcc gaagcgcgtg aaatcctcct gcggcagata cacgcgtccg
    13621 cactcggcgt cgttgcccag gtcgcgcaga atgttggtca gttgaaaggc catgccgaga
    13681 ttcaccgcat aatcctgggc gcgcggagac gtagtgccaa acacgtgcag gcagatcaaa
    13741 cccacgaccg aggctacgcg ataacaatag agcgagagtt gctcgaacgt ggcatatcgg
    13801 gtggtcgtga ggtccatctc gacgcccttg atcaattctt cgaaatacgc ctgcggaatg
    13861 gacaattcgc gcacgtgtcg ggcgaggctg atggtcaccg gcaacgtcgg cgtcccctca
    13921 taggctgcgc tcagttcgcg gcgccagcga gccaactcct cttgcggatg gctaccgggg
    13981 ggtggttcgt cgacggcatt gtccacctcc ttgcagaagg catagacggt gtacatggcg
    14041 tcgcgacgcg cctttggcag gaagagaaaa gaatagtaaa aattgctgcc gctctttttg
    14101 gtcagggtgg tgcaataggt ttgcgcttcg gtcggtgtca tcatcgtgtc attgacgtcg
    14161 gaatgtcatc ggaggtgagt tgctcgacgg cctcgggcag accatgattg aaacaagccg
    14221 ggtggaagat ggccgccagc tgttccacgc catcgatgag tctcgggcct ggtcgactga
    14281 agtacgagag cgcatcgacc agatacacct gtccccgtcg gacagccgga aggtccttcc
    14341 attcagggcg gctcgtgagg gaagccagtt ccattttggt gcgggcaaca gtaaacccgc
    14401 acggcatcag cacgatcacg tccggttcgg aggcagcgaa gacgtcccag tcaattttgt
    14461 gagaggcggc accggcctct gccagagcat cgataccgcc tgccgctgcc accatgtcgg
    14521 gtacccaatg accggccgtg tataacgggg acagccattc gaggcaggct accctcgggc
    14581 gcagtgattc tttctctatc cgcgcgtgca ccgcgtcgag cctgccacga agttggctca
    14641 gaaaccgggt tgcgggttca gcctggccca acgcgttgcc aagcgtggca atgtccaaca
    14701 ggatatcgtc gagccgtcgc gggttaagag tcaccaggcg cggagccggc gccagcttgc
    14761 gcaagatccc cgttagctgt gtcggagtga tcgcacagac atcgcacaaa ttctgcgtga
    14821 tgagcacctc gggcttggct gcaagaagcc ctcgttcgtc cagttcgtag aggccttcac
    14881 cggctgagag cattgtgccg acttgcgcat caatctgttc actggctagt gaccggcttt
    14941 cgatgcgagc gcggaccatg acgggggcgt gacttagctg cggagggaaa tcacattcat
    15001 ggctgatgcc gaccagttga tcggcgagac ccagcgccgc aacgacttcc gtggctccgg
    15061 gtaccagcga acaaattctc ataaacggcc ttgagccggt ccccagaggc gcgatcggag
    15121 gtccgagagt ctggtgtccg ccaatgaatg cgtgatggcg tctgcctcgc cgagatcctg
    15181 cactgtgtgg gtgttggcga ctgcaaggac tttcatgccg gccgctcgcg cggccctgat
    15241 cccgggcagg gaatcttcaa tgaccaggca gtcgtctgga gttaatgccg gttgcgacgg
    15301 ctgccgattc aaggcggcca tggcatggag gaatggctcc ggcgcaggtt ttccccgagt
    15361 cacgtcctca gcgctggtga tatggagaaa ggctttgcgg agcccggctt cctcgagaat
    15421 caattcgatt tcgttccgca gcgctcccga agcaatggcg agtggatacc gggcggcagc
    15481 gtcatgaacc aactcgcgca cgccagggaa aatcgccaga tgttctctga ccgcggacag
    15541 atacgcctga gccttacggt ccatcaattc acccaggagt gtgggcaacg cctgccgctg
    15601 atgcgactgt aatgcggcca ggaaacaccc gcgatcgtca aagccgagat aatcggtgta
    15661 gtactcttgt tctgtcaggg aaatatcgat gccggccaaa acctgtcgaa gggccgcgaa
    15721 atgcagcggc tcggtgtcgg caattactcc gtcgaaatca aaaatgaggg cgcgtaattc
    15781 gctcatgagg tccgggccta tccttatgcg gagatcgtct gagaccggtt tgccgattat
    15841 gcgggcgcag cattatagca agggtggcgt ggcaaaaaaa gacgacggcc cgttccaagg
    15901 gaaacgggcc gtcgttcgcg aagcgaaaag caggaatgta cttagcgttt cagcctgaga
    15961 ctgcgttcag aaatccaacc ttttttgccg tcctgggtgc gcactgagta gatccagccg
    16021 tttttctggt attccccgtc aagaatcgtc agcgtggtgc tggccggcac ggtggtgctg
    16081 gttttcgtgc cgatggaatc ggcaaataaa ggcatcggag acttgggcct gctgggatca
    16141 gcgatcacca tcacccgctg accttccgca tacaacggac ccgccactct gacgggcgcc
    16201 gaagccggtt tcatcgcagg aacgggagca gggaccacag cagcgggtgc tggtgccggc
    16261 attggagtcg gggcttccgc gggcggcggt ggcacgctat cgaccacggc gggcggagcc
    16321 atcgatggcg caggtgccgg cgcaggtatc ggtgccgcag gtgcctgtac ggcgggcgga
    16381 gccggtttcg gggccggcgc cggcggtcgc tgggccacgg gttggctggt ctccgtgcca
    16441 ccatccagat acggctggat catctgcatg gctgcgtcag gatccatggc cacatagccg
    16501 atgcctccta ccagaaggag cagcagtatc cagaggaagg gacccttctt ggatcgcttg
    16561 ggcgcattca tcggcggggc cggtggcggc acggcttcat ccaaatcgtc ttcagtgaat
    16621 tccaaatcag gctcgggctg gcttgcgaac agcagacgcc agtctgtcca cacactgcct
    16681 atcgcaggaa tgcccgtaga tgacaccatc ggaggacctc ctcattcgga atcatggcga
    16741 gagtctgtct aatcgtaggt acttatcacc cgttctccga cctgtcaatt ttgttcgaga
    16801 aaacgacgac atgactttcc gggcacggtg cgcctcgcga gcggagctca cgacgcctga
    16861 tgagtcagcc gcgcaggcga atacacgatc attccgatgg ggcattcgcc gggtcatcgc
    16921 gcacatgcac aagggcttgc aagttcgaac ttccccgttg cagaatcgcg caccacctgt
    16981 gcacggagac cgatgagatc ctaccgagaa gagctctggt tcgagacgaa aaccaggcgc
    17041 gcgtatctga acatcacggc ccaggttgaa gccgccgtgc gaaagagcgg cgtgcgtgaa
    17101 ggactggtgt tggtgaatgc catgcatatc acggcgagcg tctatatcaa cgacgacgaa
    17161 gccggactgc tccaggatta tgacacgttt ctggaacggc ttgcgccgca cgacgcgacc
    17221 tatcgtcaca ataacacggg cgaggacaat ggtgatgcgc acatcaaacg gcagttgatg
    17281 gggcgggagg tcgtggtcgc gatcacggat ggtaagctgg acttcggtcc ctgggaacaa
    17341 attttttatg gtgagtttga cgggcgacgg cgcaagcgcg tactggtaaa agtcatcgga
    17401 gaatgacgac gcgatccttg tacggagacg ctgcgtcggc cggagcgata aggaaccgca
    17461 ttcgcgcttg ccacgtatgg gaaagtttgg caacatggac aacaggatga acgacgacta
    17521 cctcgcaaca cgaacgcgca tcgacctggg aggccagcca tgttgagttg gtcacgaccg
    17581 gacccgctga cgcgagatct gatccatctc attaacgccc gacgccacga tcacggtgat
    17641 accgacgcgg cgatcgatac cctgcaagcc ttcattgagc aggggcggga gcaggatctg
    17701 ttgaccgtgc tggccgcact cgacgaggag atggcggaat ggatgttcga cctggtcgcg
    17761 gaggcctcct gtgccgtgat catggacgga cctgatgaag agaaaaacgc ctatgcgttg
    17821 atggcggcac tggagttgcc ccttcaggcc aatctgggcc agcccctcgg attaaagatc
    17881 gaccccgtgg cgacggccga tctccttcac cggcatcttc acattcccgc aggcgtggtc
    17941 gtgcgtgtcg agccgcggtt gctgaccgat gcgacactcg aagggctgac gctccaaaca
    18001 ctcaacgagc acctcgcaag tatcgccgat tcccaacgca cgcaggccat tgcctcccgg
    18061 ctctatcctc cggttgaaaa tacggccttc ctctttttcc agctgatcgg gatgccggat
    18121 cccggattgc ccgactcatt ggaagagccg gaacgtcgcg ccttgctcga gggcctggtg
    18181 acccaatgtt ccgaactggc gctggccccg gacacgctgg aggtgccggt gtatgcgtcg
    18241 tatgccctgt tcgatgccca gaatgccgtg cggctgcatc gcctggccga cgaaaccgcc
    18301 gctgtctggc gtgacctcga tccgatggtg cgggctcgca cgatcgtcca cctgcacacg
    18361 cagtgcggga acggggtgta tatgccggtc tggacagatc tctttgatcc cgagttgccg
    18421 gaggaccatg gtccggtgtg gacttcaccg gatgtgtggg tgttcacggc cgacctgccg
    18481 atcgagtggc cgggcgaaat gctgacgaat cgtctgctcg ccgagggctg cactcgcttc
    18541 gagaaccaca tcgtcgaagc gcccgaaaac tgacggtcgc tgttccgttt gccgatcgag
    18601 ttggatactc tcatagagga gtttcttggc tggctcatcg cgaatcggcg ctgtcgtgct
    18661 ggtgaccctc ctcagttgtc tggtgttagg cggtatctgg ggagtcgtcc tgtttgtcct
    18721 tccgcaaagc gagtctcctc ccgaggccct gccggtgtcc gttcccacgc ctccgcttcc
    18781 ggttcctgcc gcgacgcagc ccccagaccg ccctatgatc gatgatgctg ctcgccccgg
    18841 cgtggcgaat gctgagcctc cagtcgtcgt gccgcaacct tccgttccgc ctgttgccga
    18901 ggccgcgcgc atgcccatcg gacgggaggt caaatgcgcg ctggagatgg atgcgctctg
    18961 tcctgaagag gaaggggacc ggcgggcctg tttgcaacgg aaggcagccc agatgtctca
    19021 gccttgtcgc ccgatgctgc gcgacaagct tgctcgaatg aaagaaaatt ggcagcaact
    19081 tcgagtggcg tgcgaagcgg atcgacggca gttttgtcgt gaggtgccgt cagcgggcgg
    19141 cgaattgttg cttcagtgct tggaatcgca tgcgcaagaa gtgtccgatc agtgctttca
    19201 attccttccg aagcgcggtc gtctgctcaa ttagtcaggc ggcgtggctc cgttcgtctc
    19261 gcaacacaaa gcgcatcgta attcccgcga cttccaccac gtctccatgc cgcagggcat
    19321 gttctcgctg aaccggccgg ccgttgacca gaatgcgttt cccgctttcc gtctggctga
    19381 ccacataggc ctctccgcgg cggctgatca tcgcggcggt tttcggtgca aaccagccgg
    19441 tgagagtgat gacggcatca tcctgcgcgc cgatcagtga gacctgtctc gtcaaaggat
    19501 agtgcgattg accggtccgg ccagaaagaa tttccacgat tcccaccttg cgtgcgggag
    19561 ccggtgactc gcgtggcgcc tgagatggca ccaccatggt cttgtcggcc acgagcatgt
    19621 ctgcggggat gatgggttct gctgccagtc ctggcttctc gcgaaaaatc acccgatgag
    19681 ttccaaaccg gagcgcatcg gcatcctgca attgacgccg atcgatgggg tgttcgttca
    19741 cgaaacttcc gttggtgctg ctgaggtcct cgagaaacag cacaccttga acttggacga
    19801 tgcgggcatg gtgcgcagaa accgccggat cttccaggca gagatcgtta tccggcttgc
    19861 gaccgatggt gaagggggtg cgcctaattt cacattctct cgtgccgcca tgaggggatc
    19921 tcacgagcag gaggggtggt atcgagtgct gagacatgga ggctccttca aagtctatct
    19981 aggctggggg cgaaaccaat tgggtgtaaa tgcggtccca gaggctggtg ggccgttgcg
    20041 tcaccagcgc cacgatgacg gtcgcgttgt cggtgccgcc ggcggcattc gagagagcga
    20101 tgagacggtc tgccatagcc tgaggatcga tggattcctg actcactcgc aggatgacat
    20161 cggccgtcac tccagcggtc aatccatcgg agcagagtaa caggatatcg tcgggcagta
    20221 ccggcatctc ccctaactcg acgtccacca ggggatgcac acccaccgca cgggtcagca
    20281 catgtcgatg cggggcgcgc gccatgtctg cagcggccaa gagtccggat tgcatttgct
    20341 cttgaacgag cgaatggtcg accgtcagca actgcactgc gccgtcacga atcagataca
    20401 gtcggctatc gccgacgtga gcgatcgaca gaacggctcc agtaagccgg actgcgacga
    20461 ccgtcgtccc catcccggca tattcggtgc gtcgtgccgc ttcctgatgc acccggtgat
    20521 tggcgaggcg caccgcgctc gccaatcgat tggtggccga agaaaactct ggacgggaaa
    20581 tgcctatcaa cgggagggac cggtctgtct ccgcctccgc aagatggtga gcaatagtct
    20641 caaccgtgcg gctgctggcg atttccccgg cccgatgccc gcccatcccg tcgcatacga
    20701 caaaaattcc ggcggccgga tcggcatgat agcggtcttc gttgaggcgg cggaccaggc
    20761 cgacgtccga agcggctcca tgtgtgacgt gccaggcgtg catgggcgtg gttatctcgg
    20821 catcacctgg aagacgtcac ggaggtcttg cgccatgtcc cccgcatgcc ggtagcggtt
    20881 ctgaatctct ttctgcagcg cccggtccag gatgctttgg acgcgcgtcg gcaggtcgcg
    20941 gcggtacttc atcaacgggg catgtggaga cttggcgata cgcatgacca gcgccggcat
    21001 attctcggca tcaaagggcc gttctcccgt caaaagttcg aagagcacaa taccgagtga
    21061 aaagacatct gagcgtccgt ccacgtcttt tcctgtcgcc tgttcaggcg acatgtaccg
    21121 cggcgtcccg acgatcatgc tgctctgtgt ttttgaagcg ctcgccatct tggcgatgcc
    21181 gaagtccatc accttcacca ggcgttgctt gaggatcatg atgttcggcg gtttcacgtc
    21241 acggtgaatg acgccctgac tatgcgcata gtccagcgct tcggcaacgg tggccacgat
    21301 ctgcagcgcc tgtttggctg ggaccagcgt cgatttctgg cagtaacagt tgaggaggac
    21361 tccctcgaga tactccatgg cgatataggc caggccgtcc tgctcgccgg cgtcgtagac
    21421 cgtgatgata ttcggatggg acaggcgccc ggtggattcg gcttcgcgaa agaaccggtc
    21481 tcgaaactct ttcaggtctt cttccttgtc gatgtcgtcc agccgcatgg ttttaatggc
    21541 cacaaatcgc tggatcgtcg gatcttttcc caggtagacg actcccatgg caccgcgccc
    21601 cagttccttg agcacctggt atcgtcccag cgtagacggc acgccgtttc cgttgcgcac
    21661 ggctatgccg gctgtcgggc gggggggggc ggcgggaatg gcttcgtctg ccgcacgcac
    21721 ctcgtcgggc ttctccgctc gcaccggttc ttcggcaacc gcccggcggc gtggacgcca
    21781 ggcaaacatg ctggtcaggg catcgacatg acggacccag gcaaacgtca gccaggtcgc
    21841 tgagccgatg aggccgccgg tgaccaacca ggtatagagc ggctgtctag gcgggccgag
    21901 cacagatatg ccgtgtgctt cataggcctg gaatgcggca atgacgatca gaagggaggc
    21961 ggacgggaaa agaatggcgc ggagaaaggt ttgacccttg ccgttgtcct tcaggtcccg
    22021 tctggctcgc gaggccgcag accagatcat gagcaggcag atgccgtctg caatgaaccg
    22081 aacgacttgc gcgacgttca tgcccaacgg ccggaggtag gtgtgcgtaa acagcgggag
    22141 ggtcgcgaga aatggcccga ccagaaccgc aaggagaatg acgatgaaat acgggccgat
    22201 ccagccccaa gatgtggtca tgtgttggcg cggtacgtcg ttgtagccag tgtaacggaa
    22261 ggtcgtactg tgtcaacgaa acgagggaat cacgcaccgc cggggcagag tctcgtggca
    22321 tccggatgag gacagaggag gcgtgtttgg gagaaaaggg ctcaggacga ggtggcggct
    22381 tttctgagtg gatcgagcaa tttctgtgag actttgagct ggccgcggcc gatcccgacc
    22441 tcaatatccg gtttggtgac gccgtggaaa tcgctgccgc cggtgacgag gagatcgcat
    22501 tgcctggcca gatccaggta ttcggtcgtt tgactgggcg tatgggtgct gtaatgaacc
    22561 tccattcctc ccaatccggc agccttgagg tctctcacaa gtacccgcaa cccttcggcg
    22621 gatgttctga cccacgtcgg gtgggcgagc accggcacgc cgccggcttc gcgaatccat
    22681 cgcacggctt cggccggttc cggtaattcc cggtcgacaa aggccgggcg gccgtttgcc
    22741 aggtatcgat cgaaggcctc tttcgccgag gtcacaaatt ttttctccat caaaagacgg
    22801 gcgatgtggg ggcgccccac ggattccgtg cctgccagag cccgcacttc ttcataagta
    22861 atcggaatgc ccaattcgtt gaggcgttga acaatcttag gattacgaag atgtcggctg
    22921 tcgcgcaatg tgctcaggcg ttgcgccaat aacggatcgg tccaattcaa aaaatatccg
    22981 aggatatgca gttcgctctc tccgagccgc gaactgattt cgacgccggg aaccacctcg
    23041 atgccgagtt cttttccgat agctgtcgct tcgggaatgc cgtcgacaat gtcgtgatcc
    23101 gtgatcgcca gcgccgtgag gccggcctgc ttggcaaagg ccatgacctc ggccgtggag
    23161 caactgccgt cggagtgcgt ggtgtgcaga tggagatcaa tgcggctcat ggtgatgtct
    23221 ctggccccgt tttctgcgcg accagtcgcg cggtgtagca tggcccgctg ccatgacctc
    23281 cgtcgtgttc gccctcgtca tagtgaagaa cgcgcagggg cgaggtgagg cggaggagtt
    23341 cgttctgcgc caggcaaaac tcccatcgcc gcggatgctg atggcgaaaa taattgtcga
    23401 tggtaaatgt ttcgtagagg aggatgccat tgggtttcag ggcgtcaatg atgacaggga
    23461 acagaggccg atgcaggtaa aaaaagacga cgatggcatc ataacattcg tggcccaagc
    23521 tgggcgggtg atccgcagag gcttccaggt cgaggacgcg tgtcgtgaga ccggagagac
    23581 gctgtctgcc cgcagcggct tccaatgcgg agagggcgtc ggtgtctcga tcgatggcct
    23641 ccacctgtga cccctgggcc aggaggtaca gggcatttcg tccgcgtcca gcggcgacgt
    23701 cgaggatgtg tcccttgggg agacgcgcaa gttgttggat gagaaacggt gcggcgctca
    23761 gtgcctggtg tgttgactga tgggcggcac gcgtgcgttc aaaccggatg ccgactgacc
    23821 cggcgaccat ggccagcggg gcatggactt ttcgaatcca gatgcggacc cgatcagccg
    23881 gaaattccgc gaataacatg ccgaccaatt gctcggccag ggtttcaaga agctggcagg
    23941 tgaggctcgt gctgagtgcg acgattcgtt ccgctacttg agcataatcg atcgtttcac
    24001 tcaagcgatc cgaaaccgcg gcggcttcca cggcggcgtc cagttcgagg tcgacgacga
    24061 tcagctgggg tttgcggcgt tcttcctccg tcacgccgca gcggccgtag aactccaggc
    24121 gttcgatgac aatccgctct gccatggcgg cattgtcgta gatgggcttt cagactgtca
    24181 agcaaagcgg gcgcgccatt cagctaggag cgtgcgaaga gacgcttcgt gagccaatgg
    24241 gcgggcgagg cgaaccggcg ggctgaagca acgatcgtcg caggagaaca cttcgccgtg
    24301 aagtcttgca ccatccgtct cgcacccctg tacagaaact cggtcacgtc actgactgtc
    24361 ggttgtgggg tacggttcag atgaagctgc ttcaattcgg ccttgtaggc tttgcggcgg
    24421 cggcgtgtgg catgaatcgc cacggccgcg acaacggtca acagcaaggg attcgagggg
    24481 atggagaaca accaccgcca ccattcatag aatgccagca cggtcaggca gaggggcagc
    24541 gtcaacacat cagcccatgc cgattgtacg gcgcggatct gttcgtctat cgattgaccg
    24601 ttgaaccggc ggggtatgct ttgcagggac gtgcgtcggt ttttcgaggt gaagactcgc
    24661 gcggggagaa acattccggg tacctccagt agtgaacgac atctttcagg aggcattgta
    24721 ggacaggttg cacaatcaga atagaaaaga cggaatttct gcgggtgtcg actcggtagt
    24781 tttacccgta tgtattctgg tcgcttaacg acctgcgagc aggctcagac gctgcgtcag
    24841 atagggagtg aggtaatacc ctaaggcgga gatcagcacg ctcgcggctg cgccgatcag
    24901 accgtacacc aacagtccgg caaagggcag caggaagacg atgaagatca tcacgacggc
    24961 gatgggttta tgaccttcgt aaaaggccgt gcgtcgttca ggtgtgagcg attcagagaa
    25021 cgagagggtg ggcttatcga tcattctgct gcgggtgtat tccacatata cgatgtgtag
    25081 agcgaacgaa ggttgtcatg tccagcgacg tcggcggcgg acaggtgaca tacgaatcga
    25141 cgtgcccgcc gtggctttca attcagtgga gccgatgcag cacgccttcg gcgacaacgg
    25201 agctcggatc gccaccgaca atcaaatcct tatcaccgaa atgggaggcg gtccgcgtcg
    25261 tcacgcgcat cttcattccc tcggggacca gcgtgtactc aaacaaggcg gcttcctggc
    25321 gttggtccat atacccctga ctggcgcgtt cgaacgagcc gataatactg atcgacacac
    25381 gccccgggcc ctgttcgaaa cgcttcacag ccatccgttg cacaccgcca cctggtggca
    25441 cgtattcaat cgtttgcccg acgtgtgaca attccacctg gcgtgtgacg cccttgttgt
    25501 tggtatagga gtagagaggg aggccctgcg gactgaagtc gatttggact tcagcccggc
    25561 tgccgtctgg gctgctcagg acccctttcc acgcgccggc caattccgcg gcctcagtgg
    25621 agccggatac ggccaggacg ccgatgatcg ctgcgatgtg cacgaagtgt ctcatagtca
    25681 tgagcctacc ctcctgtgcg atggccggtc gcgcgccgag ctctcttata ctttgaatgg
    25741 ctgttgccgg agaaagcgcg ccgccgcccc gagcgtaaac agattgggcg actggctggc
    25801 gggcagcccc gccttcactg ccgcatggaa tttggcatag ttcccccgat agttcccgtg
    25861 gttccacgcc ttcagcacct gttcggtaaa ggcgccattg tggtcgccgt ccatggacgt
    25921 ctggttgtcc tggcagcctg aaatgagaat cacggcagga tgaaattctt tggcgatggc
    25981 cgtgaggcgg gggctcacgg ccagttgggc cagcacgctg tccggatccg tcgtcccggc
    26041 cttgccggcg gctttcgcca cgtcatgctg caatttgttg tagaacgcct gatgttcccg
    26101 ataggtccga atggcgacgg cagggggcat cattttcgag cgtcccgtca ccttcgtccg
    26161 atcaatcggc ggtgggacgg cgcgggtgac cgtaccgcta tggcagctgt ccgacaacac
    26221 aaggattcgc acgcccgccg caaagcggct taattcgaag tacaactcat cgtcgataag
    26281 ctggccgtcg tacagacacc aggtctcatc ctgcttgtcg atttcttctc ctgtcacgtc
    26341 gggcacctga ccgccatggc cggaataggt cagaaaaaac agatcgcctt gtttgagctg
    26401 cttcgcggct tttcgcatcg cggccagcat tttggcgcgg gtgccttgtt tggtcagcag
    26461 cacggtggat ttcatgccct gcgatttggc gatggcggcc atgtctttgg catcgaactc
    26521 acaggcggcc agttcaccgc tccagccgcc ataagcggcg ggactcacgg cgttgaggcc
    26581 gatgtgtaac gagagggctt tcggctttgc agcggatgct gtggtggttt tggccatacg
    26641 tacctccttg gtcggctggc tccatgtctt gataggttcg gatgaagagc aaatgatggc
    26701 ggtacggtaa tcgttacgcc gaaatctgtc aacggagctt cgtgggaaat actggtattg
    26761 tggccgtccg gtgacgctca tggatcggga cgagatgtgg cgatgaacca catgattagg
    26821 tgtcctcctc ctcgatttct tcgattgact ccttgatcgc agccacaaat gtttccggtg
    26881 cggcgagggc ctgcgcagtc agttggaatt cagagaccag caccccatct ttatccacga
    26941 cgatcagccg atacccttgt ttcacgacca cgacctttct ctgcgcattt cgcaggcaga
    27001 tgctattgac gagaggccct acgggcaggc tactgtatcg aacgatcagc cgtcatgtct
    27061 tcgttgggag gcaggatgac cggttggtgt tcgccattca ggcctggtag gatgacacaa
    27121 tcaccagtac gattcgatcg tccaaggaaa ggatagtcac ttgcatacaa ccaaactcat
    27181 agccgcattt ctcgtgcttg tgttggtcgg cgtgccgacg atttcttttg cgaaggcgcg
    27241 agggggatca gggggaggat tttccagcgg gtcacgaagc ggatcttcag gaagcatggg
    27301 cagtcgtgga tcgcgcacct atcaggacaa tggggccaag cccatcgaac aatccacgac
    27361 agccaggcct tccgccaccc cgccgccttc gatggcgggc aatccttcga ctgcatctag
    27421 gcccgctccc gctgcgcaac cgtcatggtt tcaacgcaac ccccttctgg ccggcattgt
    27481 cggaggtctt gccggaacgt ggctcgggca catgattttt ggtgcgaccg acacgagtgc
    27541 ccgcacgacg gaaggtggtg agggcgaaca ggccgccgca ggggcaggct caagcggcat
    27601 gttgctgctg ttgatggtga tgggtggcgg tgcactctgg tggtatatgc ggtcgcggcg
    27661 taccccggcg ccgaattttt ctgggctttc gcgaaccagt gtcgcgagcg gctcattgct
    27721 ggccgaatca tcgcgtgcag gattcggggc ggccgctgtt gaagaggaga tgagctcaac
    27781 agacaaggcg gcgtttcaac aagcgctgat cgatattcaa accgcctgga gtcaccagga
    27841 cctcgccggc ttgcgtcgcc tcgtgacgcc ggaaatgctg gattatttca gcaccggtct
    27901 ggccgagcag gccagtgagg gtgtcgcgaa tcgcgtggaa gatgtggtct taggacaggc
    27961 ggaagtccgt gaatcgtgga gtgaaggtgc gacgcaatat gcgacggtca gtctctgctg
    28021 gagagccaag gactacacca tctctctggc gaagcagccg ggcgaacagg gctacattgt
    28081 ggagggcaac gctgagctgc cgatcgaaac aacggaggta tggaccttca tgcggtttgg
    28141 agccgggaag tggcttttgt cagccattca gcaagtgtca tagcggaatg ttgaaaaatc
    28201 ccgccagctt cgttctcacg tcgttcagag gatcaacgta cggctcgagt acgtctcacc
    28261 tcttccctcg ttgcggcctt gctggacggg atttttgaac atcccgcggg cctcgtgaat
    28321 tgcgaatgga tcggtattgt tccttggggt agtcacaccg gtggctgccc ccgttgttct
    28381 ttggttagcg tgtatgtgat gcctgccgca aacggctggc ggcggtgtct tcaataggca
    28441 ggcaaccttg ggcttgagaa attgcctcga gcagcgcatg ccgctcctgg tccgtctcct
    28501 gcaccggcgg catgctgtgc acgaattgtt ctgccgcctc caccaagcgt tgcagcctgg
    28561 tggccatttc gtccgacggt gcctggagtt cggacgaagt cttgcgagcc agtgaccgct
    28621 ttcttctcgg cccgacatag atctgcgggg ccacgggatt atccttctcc aattccggtc
    28681 gtctcgatat cggcatgcgt cctcccaaat ggcaaacgag taatgcccac tctactcgat
    28741 tctgccgcgc gtttgcatgg tcgaaatcat tcttgtctca atggagaggt gagcctgaat
    28801 ccgtcaaacg attcatggct tgtcacactg attatctgga atcgtaaccg tgtcatggct
    28861 gcgattgacg gggcgccaca gggatgacgg gtgaggtggt atgtcgactc agatagaccg
    28921 ttaggggaaa acgagtgcac cggtcaactg tatggaagga ttcagggaaa tcgcatcgcc
    28981 tctgatcgat gcatcagtca gtctctcatt tctccttcgg tgaattatag gcacgactgt
    29041 tgcgtcagta attgccgttg ttgtaacgcg aggtgacatt cattcggcca aggctgtcag
    29101 cgatgccggc cagaggagtg aagcatgcgc cagtctctcg gaatgcgttg ctattttacc
    29161 gccttcgcca tgtttctttc ggccctcgtc ggccggtcct gggcgtccag gagataggtt
    29221 gcgctagcaa tctcccggtt tcgtgtctgt cagcggtgtt ttccctcacg cagtcctttg
    29281 tctgactgaa ttcggttcct tcctccttga tcccgctcgg accagatact cgcctgccgt
    29341 tcaacgcgct cgatttcgtt gacgaggcgc gggcatcggc cggatgtcgc ttgactccca
    29401 ttttccccgg ctcctataat ttggcggttt tgatgcgaca accagctgat ttggcataaa
    29461 gaatggccga ctttacgcat ttcaacgaat cggggcgggc gcggatggtc gatgtcgggg
    29521 caaaggcctc gaccgagcgc ctggccaccg cgcaggccat cgtcttcctc caaccggaaa
    29581 cccttgaaaa aattcagcaa ggaaagatcg caaagggcga tgtgttgtct gtcgcccagg
    29641 tggccggcgt catgggggcc aagaagaccc cggatctcat tccgatgtgt caccccattt
    29701 tgttgacgag cgtcgatatt tcgttccagg aaatcgctca acgggaccgt gaggggcgct
    29761 gctcgatcgt gatcagtgct accgccaaga ctacgggccc gacgggcgtt gaaatggaag
    29821 ccatgacggc cgcttcggtg gccgcgttga cgatatatga tatgtgcaag gcggtcgatc
    29881 ggggcatgag cttcggcgac atctgtctgc tgacgaagtc gggcggcaag tccggcacct
    29941 atcaacgtcc ggttccagtg taagggcgtg acatggtaac ggttcgactc ttcggcatga
    30001 cgaaaatgtt ggcgggaaac caaggtgcct tgtctctggc cctgccggaa ggccgacggg
    30061 tgaaagacct ggtgtctgtg atcgaagccg cctacccgaa gatcggtgag ttgctccaca
    30121 agaaaaaagt gctcgtttcg gtcaatcagg atatcgcgca tgaggatctt gaagtgcgtg
    30181 acggggatga aattgctttg ctgccgccgt ttgccggtgg aagtgccgga tgctgaacaa
    30241 gcctgccagc ggcgtgctcg ctttgcgcgt cggctcgacg tactcaaagg tacgcctcgc
    30301 ctccgtgcgc gctgcggcct tgccggacag gcttgttgag catcctttcc gcagagccaa
    30361 cgggaaaccg gagggagaga atcgatgagt cttgaacagg tcacgaaagc cgaccagccc
    30421 gttaccggcg acgaggcgat gctggttcgg gtgcagcgag aagatttctc gatcgacgaa
    30481 gaactctgcc gtgtccgcgc tcgttccaag cgcatcggcg gcattgcgat ctttctgggg
    30541 actgccagag accggtcgaa ggggaaagat gtcgacggca ttaccttcga gcattacgag
//
LOCUS       DAOQEW010000021        30471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_327_length_30471_cov_9.079070, whole genome shotgun sequence.
ACCESSION   DAOQEW010000021 DAOQEW010000000
VERSION     DAOQEW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 30471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_30471_cov_9.079070"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..>1438
                     /locus_tag="PLS35_04065"
     CDS             122..>1438
                     /locus_tag="PLS35_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNI17863.1"
                     /translation="MGENHSAKSPLCVDLDGTLIKTDLLWESLLALLKQNPLSIFLLP
                     VWLLRGRAYLKHQIAQRVTLDASTLPYDQELVEFLSKERLAGRELILATASHVSFAQV
                     VATHLGLFDGRVFGSDSSVNLKGARKVALLVERYGTRRFAYAGNSTADFPVWAEASEA
                     IVVNASAGVASRARTLTTVSRVFRAPVNLVKQVAKALRVHQWSKNVLVFVPGVASHQL
                     ANGWLLLQASLAFLSFSLCASAVYIVNDCLDLESDRRHPRKRNRPFASGNLSIPFGLA
                     LAAVCLIGGVLLALALPSVFLLVLGGYLALTTGYSFYLKQFVLVDVIVLAQLYTVRVY
                     GGGAATGVPPSHWLLTFSLFLFLSLALVKRFTELRLMSQVEGKGLHGRGYWVTDLEHI
                     SSIGTASGLLAVLVLALYISSKEVLLLYSHAEVLWLVCPVMLYWISR"
     assembly_gap    1439..1538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1539..2273)
                     /locus_tag="PLS35_04070"
     CDS             complement(1539..2273)
                     /locus_tag="PLS35_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI17864.1"
                     /translation="MPNQVALITGGAKGIGRAIALDLAAQHWCIAICYRTSAAAAEET
                     SAAIMARGGQALALQCDVSDPAASKQLVAQVEAQWGRIDALINGAGPYHRVNLFDETT
                     TGWREMFDGNLHPIFYLGQAVAPGMKARKHGRIINFSMANADQMVAQPEVTGHYIAKA
                     GVLILTRTLAKLLAPYGITVNAISPGFIDSGSAPAEELAGVVKRIPAGYVGSVNDTVA
                     AVRYLLSEEARYVNGANLHLSGGWGI"
     gene            complement(2285..3037)
                     /locus_tag="PLS35_04075"
     CDS             complement(2285..3037)
                     /locus_tag="PLS35_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNI17865.1"
                     /translation="MSPQPSDRKQPASAALVIFAKAPVPGQVKTRLCPALTEDEAATL
                     HGSFVLDTLERTKAAASTFKLSFDRILACAPSSTHVFFRIMEARYGVTLFDQEGDDLG
                     ARMQHTFAQLFARGYRHICLVGTDVPSLPLTHYRDAFAALTRHDLVLGPANDGGYYLI
                     GMTRPHPKLFAHIPWSTDQVLALTQQNAAAEGLNTALLPSWNDVDTLEDLQALIEDCV
                     ADKKRPKKERIYSTRTAGTLELLAKRLRERRG"
     gene            complement(3041..3757)
                     /locus_tag="PLS35_04080"
     CDS             complement(3041..3757)
                     /locus_tag="PLS35_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNI17866.1"
                     /translation="MTIAVIMPVLNESRCLAHILAHTQALGFDEVIIVDGGSSDDTFS
                     IAQSFAKQCDTDPLRASGSMRVLTAPRGRARQLNTGAAASACDVLLFLHADTLLPSNA
                     RRAVLTALSDAAAVGGRFNVRFDSSRLTALVVSSLMNVRSRWSGIATGDQAIFVRRAV
                     FETMGGFADIPLMEDIDFTRRLKRTGPLVSLSDEVVTAFRRWEQQGPLRTILLMWTLR
                     FLYWIGVNPHRLQHFYSMVR"
     gene            complement(3802..4209)
                     /locus_tag="PLS35_04085"
     CDS             complement(3802..4209)
                     /locus_tag="PLS35_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17867.1"
                     /translation="MPPPSDLPDPSTRPSWPPPHRPDDESTSERQAHVFGMMIGTALM
                     FIGFLDVFLSISGGFEINVVPLLIYFSGIAVWANAVVENPTVRYSLMAGSVLISLAFF
                     HYGEVLFWHKQVVFWSTVAIVMYFMFKDPKPTA"
     gene            complement(4212..5363)
                     /locus_tag="PLS35_04090"
     CDS             complement(4212..5363)
                     /locus_tag="PLS35_04090"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HNI17868.1"
                     /translation="MKRAASSKGILPYQHITQLITRGAIKSDAPIEDRQIQPASLDLR
                     LGKKAYRLISSFLPELSAISSRLNVLDFYQSDLVMYEMDLTQGAILEKGHVYLVPLLE
                     HLELPATLRARANPKSTTGRLDVFTRVVTDLNAGFDEIRAGYTGPLYLEVVPRSFAIK
                     VRTGDCLNQIRFVRGDATVSDTALQKLHAADPLLFRNASSPKPLPQKEFRTERGLFLR
                     IDLTGSAHEEAVIGYRAKKNSHVIDLAKIGHYAALDFWEPLKRHRHDSLLLEPEEFYI
                     LASKERIRVPSGYAAEMVAYEAACGELRTHYAGFFDPGFGYGDGTMRGTQVVLEVRPH
                     DVPFLIHDGQTFFKVVYDRMLTVPSQVYGTALGSSYQRQALTLSKHFKA"
     gene            complement(5360..6493)
                     /locus_tag="PLS35_04095"
     CDS             complement(5360..6493)
                     /locus_tag="PLS35_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNI17869.1"
                     /translation="MEDWKRILAQSVVKPKDLAERLGVDPKEVEDIVGDYPMRITPTV
                     LATIKEKGDAIWKQVVPDRAEMADADAEDDPLEEDLMSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAIAYLREHQEVRDVILSGGDPLLLPDHLLER
                     ILKSLRTIPHLELIRIGTRVPGSLPERITPKLCEIIKKYHPFYMNLHFNHPDELTPDV
                     KRACGMLADAGVPLGAQTVLLKGVNDDPEVMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTSVETGLKIIKSLQGHTSGMAVPHFVIDAPGGGGKIPLLPGDYLVNLDEDGAVLR
                     NYENNTYHYPQPGSPQGRELPMVGAHPSWAASGNGCDGGGEHL"
     gene            complement(6608..6853)
                     /locus_tag="PLS35_04100"
     CDS             complement(6608..6853)
                     /locus_tag="PLS35_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNI17870.1"
                     /translation="MALTFYHVRWCPDCAVVRDRLSELNLSYEDVVVPDFRPMRKQVY
                     EVSGQYYVPVLKDGDTVLTETHDILAHLQTHYEKAQP"
     gene            complement(6923..8437)
                     /locus_tag="PLS35_04105"
     CDS             complement(6923..8437)
                     /locus_tag="PLS35_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17871.1"
                     /translation="MALAQHSREPLNSDEARRRCLAIRDALVHAGVYGPADTEQGTAT
                     AGSGVSPVRFRLSPTPFPLSSSEVMCFQHLGQDLLAFYRALNRLYQESARGTQPGWVA
                     AYLDQGKPESLVTFSRMNRFRNLIPGIIRPDVIPTADGMVITELDSVPGGIGLTACMT
                     RAYDDQLGLSPSPASPPSERSPLIAGRDGMLQGFAAMLRAQQGDREGCLAIVVSDEAK
                     EYRPEMEWMAQRLRDIGFQAFCVEPRAVRCTDEGLYLQDEHGMRTIGLLYRFFELFDL
                     KNIPKSELMMYAAKKGQVAVTPPYKPALEEKLAFGLFHHPELEPFWKETLTAETRTNL
                     GQLFPRTWILDPQPVPPTAVIPHLLISGRAVSDFRRLAHTTQKERQLVVKPSGFSELA
                     WGSRGVSIGHDLPQVEWAAALEQALQSFSTTPYVLQEFHKGRLFEASYWDERSDTVVP
                     MSGRVRLSPYYFVAGDKAELGGILATLCPANKKIIHGMVDAIMAPCAIAPGVAA"
     gene            8657..10165
                     /locus_tag="PLS35_04110"
     CDS             8657..10165
                     /locus_tag="PLS35_04110"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNI17872.1"
                     /translation="MTSPLVPVSPANPTRPVGQSLLQIVPSTTCLHCDVCCRFPEVDS
                     FLRPFFTGDEIRAAVAAGLTPELFPTTEGTQIDLVPNPAGEGYVCPAFDSETSHCRIY
                     EVRPLDCRLYPFALMWDAERTHVLLGWDTKCPYMRDAPSSLVDQAVDGVAQWVEADER
                     VDTLARYPRLIGRYQDDVIPLRTLARVTERVQRAKPQIRRQALTIQDRGRIEAALTVT
                     AGFQESPLAAASFAYHYIWKHRLTYTWAELQGHLCLFAESPDGIFLVLPPLGSGPIDQ
                     PTAEAFRLMRAWNGDSSVTRMENVPESFVPALRASGYETGEKEPDYLYAAADLVDLAG
                     EAYRSPRAACNRFVREHGGVLEPYDCRDQTACVSLFREWREQKLRSGSDEWANALLED
                     AVGAHETALSAHSELGLTGAVVRVGGRIRAYTMGLWLNQSVFCVLLEVADRDIVGAGA
                     FVFREFCRQAHAKGACWINTMDDSGLPSLAKAKRWYHPARLLPNYVVTEFRR"
     gene            10162..13263
                     /locus_tag="PLS35_04115"
     CDS             10162..13263
                     /locus_tag="PLS35_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17873.1"
                     /translation="MTASPLPGFPRRSYPWLPVLIVGVTIVALVIGVVMLRSIEVRMV
                     EATGENLTLASAEIADKLDRLLFERHADVRMMARAFTDRSADKKYINEYLQWMKETYA
                     PVYLWLGVTDPHGRMIAATDSSIIGQDYGRSGWFDRVRQTHTVQVDEVEIHESNHKIE
                     SVAFTAPILSVSGQFLGAVTTRIGLPMLEDVLIETVREIQQTDGAAGPFEYQFLTKGG
                     LVFVDSSSSNIDRVNLRELNLPSVLRSSSGKAGYVEEIHVRRHVPIVTGYSQTKGYSD
                     YSGLQWTILLRIDRDVILLPIRAMMWKLGIAGVVVWLPMVGLLLWATGRLREQHRQAQ
                     QESEWARAAEAALLQSQERNRVIVDTALDAVISMDAGGIITDWNAQAVNIFGWEREEA
                     LGRRVSETVIPVRDRDGHERGLRHFLETGQGALLNRRIEMMGSHRDGHEFPVEITISP
                     ARLGDAYIFSAFIRDITARKRTERQLASQYAVTRVLAESRTLEEAGPKILQAICESLE
                     WELGIFWRLDRQGSVLRCLDVWQSPGLNAEEFVLDTWRHTFALGKGLPGRIWQAGEPT
                     WIKDVVQEANFPRGGAASRVGLHGAFGFPVRVGTEVEGVIELFRREIKQPDEQLLKMV
                     ADVGLKIGQFGERARAEDALRRTEAQLQQSQKMEAVGRLAGGVAHDFNNMLTVIRGYS
                     ELVLSRLSPTDGFRKELEEVKKAADRASGLTGQLLAFSRRQFIAPKVLDLNSVIHNME
                     GMLRRLLGEDIVELCTVLDPGLGQLKADPGQVEQVIMNLAVNARDAMPDGGRLTVETA
                     NVQFGQRRNRPPMGAEPGSYIALIVRDTGHGMDEDTQSHVFEPFFTTKEKGKGTGLGL
                     STVYGIVKQSGGFIEVESKPGRGATFKIFFPRVEAVSEETESVAVGGDSVRGRETVLL
                     VEDEPGVRRLVNETLRLHGYTVLEARHGIEALLTGAKHAGPIHLLLTDVVMPQMSGPE
                     VAEKLATVRPEVKVLYMSGYPDHPAFSKGGIDTEHSFLQKPFTPTTLAQKVREVLDGS
                     KVS"
     gene            13358..14197
                     /locus_tag="PLS35_04120"
     CDS             13358..14197
                     /locus_tag="PLS35_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HNI17874.1"
                     /translation="MSEGRAATAPKGQAQDIDHYRIAREPFYADVRGEVGLFTIAAQS
                     RMPVMLKGPTGCGKTRFVQHMAYRLGRPLITVACHEDLTASDLVGRYLLKGQETVWMD
                     GPLTLGVKQGAIVYLDEIVEARKDTTVIIHPLSDDRRLLPIEKKGQVIEAVDEFMLVI
                     SYNPGYQSILKDLKQSTKQRFMAIEFDYPLPDIESRVVAHEAGVSPEIAQRLVKIAEK
                     VRNLKNHGLEEGVSTRLLIYAATLIKQGVPADRACDVAIARPITDDPDMLRSIIEVVK
                     AIF"
     gene            14243..14578
                     /locus_tag="PLS35_04125"
     CDS             14243..14578
                     /locus_tag="PLS35_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNI17875.1"
                     /translation="MTTHNSGRESKSSAAAQVVRTTPEGAVISVHVQPKASRTQCAGL
                     HGDAVKIRVAAPPADGAANEELCRFLARTCGLPVSGVEILSGTSSRQKRILLRGRSAE
                     QVRAQLQLE"
     gene            14634..15119
                     /locus_tag="PLS35_04130"
     CDS             14634..15119
                     /locus_tag="PLS35_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17876.1"
                     /translation="MHGEPGVELWPRSSVLRKIRSIIFRWSLSLIVVVVSGCAGPEPI
                     FKSTTYLQLHGQQQAQDEAAVCAVKAERAGLQHGTNRSGNAGAGAALGVIGGAAVGAS
                     AGLVGGPTGIAIGAAAGGAVGGILGLFAGTYKPLHPRQDYAAFVERCLKEKGYETEGW
                     Q"
     gene            complement(15158..16405)
                     /locus_tag="PLS35_04135"
     CDS             complement(15158..16405)
                     /locus_tag="PLS35_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNI17877.1"
                     /translation="MISRFCCNPIVGLRYGLGVVAVLTSLSMAGCLHTMEALDPRALP
                     VTTAPAQRLQSYVSFLASPALAGRQPGTPGNRQAAQYIEEQFRLVGLQPLASLEGYRQ
                     TISDRMGDNVIGVIPPIDHTARTRWIVIGAHYDHLGYPFLGADDNASSIAILIETARN
                     LTGLSRHGIMFVAFNGEELPYFGTAEMGSQFLFHHLPPEVGAAENLQAAIIMDLMGGV
                     HWAPLKDAIFATGAEKSPALYRRVTETRVSPLTIYRGGIHLVEEIPDHGHKAFSDYDV
                     FRNQKVPFLFLSAARSPRYHTAGDVASTLHYERMAATVEWLRQLLALMSQDEAPYAFD
                     AHRVEFADEVASFTSLVEQALHEETRIPGTSRWSLRKLEQDLEWLREVHGSAPTPQLL
                     ERLERISIRVQCLMADYSGCFTF"
     gene            complement(16402..16848)
                     /locus_tag="PLS35_04140"
     CDS             complement(16402..16848)
                     /locus_tag="PLS35_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HNI17878.1"
                     /translation="MAEPSKESLEKMWKYVKGFAEKSGTAMHPNPAVTNAVVLGLAAH
                     VDELGKPLCPCNFYPDKQAEAKLRRWMCACDEMQIYKYCHCLLFVNEEGLPITEYLPE
                     DHEGRQCYGLVPDPTPGKGRALRHKALPMAPKVPESPATPDSSAQS"
     gene            16980..20015
                     /locus_tag="PLS35_04145"
     CDS             16980..20015
                     /locus_tag="PLS35_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNI17879.1"
                     /translation="MAPGHSFPQLIERLADQLGPASATTMVDAIVASATRPDAPAGVW
                     LLLEELTDAAPKAARGAIEALLELQRRGLLAEVLPWLDLGVAIAADSGALALRFFKES
                     PLLLGLLDPPQRAVVLAAGLELADRQANVAVEFIRVAPEVVPVLPPADWPTWMELACE
                     LSETDFALAVEYIRQIPAIARVLSLDTVRAWMQFGMTLIVDNSLGKPDYLGALEFFRT
                     SPGILADIPDQISRHQVIRVGAQIAERSPEMGIALLSEAPSILRGLPTDEWRRQVLQY
                     GLILAERDAETALAYMRRCPELVALIGSGEGAQQKFQSWFASGMDVLEYSVEGGRAYF
                     GMESEKALGALSQAMSGVPLRQIARRVKLFAQALCGRDLRIYDLPDSLESAQPMPRAT
                     VSDDGRSIGLPSLVRRHSTYEENVRLYMVMAAHEAGHLEFGTFDLPLARLEDVSKELT
                     QRYGRPALREVRTLGDMFARYPQPGLIRDLWALVEDARVEYLLRREYPGLSRDLAVIA
                     KDAVQVRSLAHGMTVRELVVDQLLLLSTVEAGSMPLPDALKREIMHLWSLCQTIFHPQ
                     ATAEEAVRLADRLYVSMDELLAIRREAIPSPDEAESPEQSIPAPKASEDLSDTYRPMD
                     NWDYRGAMDPNLVRDRTESTPEQSPASGQGDSEGGAGLAGDSGGSSGAGTARSQQMSQ
                     EMLVPGRRQPSLVEEMLAVEGEGARPQDDQTETVKAVRYREWDAAIQDYRMNWCRVVE
                     REAVEGSSDFVEGTLAAQRGPVALLRRYFESLRPPGLRRVPGQVDGEDLDMDAVIGRL
                     ADIQAGIEPSERIYVRREKREREVAAAFLVDLSGSTSRQVERGRRIIDIEKQGLVLLC
                     EALSAIGDQFAVYGYSGQGRHLVDFVVVKDFDQLVTGRAGAKLGGMVPLQQNRDGAAI
                     RHATTKLLARQAKTRLLVMISDGRPLDDGYKDEYSLEDTRMALREARASGIEPVCITI
                     DKQADPYLRRMYGDVRYVVIDRVEGLPEKLPRIYHRLTA"
     gene            20059..20469
                     /locus_tag="PLS35_04150"
     CDS             20059..20469
                     /locus_tag="PLS35_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17880.1"
                     /translation="MIEQSPRPTVPPWLYKLFTGHQYPYVRRQAKFANRDFKPGEERP
                     EPTREAIDAKFWEIYPRCTAKILQEVKAGMIVVFHELGEYPPGGYQDLVDAPEEFLAA
                     KYGKKKIKVNFYDGDNFVCTINFKVGGWTGHEPA"
     gene            20542..21483
                     /gene="mdh"
                     /locus_tag="PLS35_04155"
     CDS             20542..21483
                     /gene="mdh"
                     /locus_tag="PLS35_04155"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNI17881.1"
                     /translation="MGRPKITVVGAGNVGGTAAQRLAEKNAYDVVLVDIVPGIPQGKA
                     LDITQAGPVCGYSTRVVGTNGYDETAGSSIAVITSGIPRKPGMSRDELLGTNAKIVKT
                     VVQELVSRSPNIILILVTNPLDAMVHVARKVSGLPKSRVLGMAGVLDTARLRSFVAEE
                     LDVPGTDVQAMVLGGHGDTMVPLVRQTTVAGKPITDRLSSERLAALIKRTQDGGAEIV
                     GLLKTGSAFYAPSASVVDMVEAIMKDQKRVVPCAMLCEGEYGLKDVIVGVPVRLGRGG
                     GESIVEYDLTVEERTALQASSDAVRDLCGVVDRLLTA"
     gene            21587..22174
                     /locus_tag="PLS35_04160"
     CDS             21587..22174
                     /locus_tag="PLS35_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HNI17882.1"
                     /translation="MKFLEDPYQTLGAGVALAVGLIVVYLGMAGVGAGEADWFGLVVR
                     WVHFLAGITWIGLLYFFNLINAGFLKSLDGPTKNIVIPKLMPAALNWFRHGATVTVLA
                     GIVLYGYLYSKGGTGALALGIGGLLGIIMMGNVHGIIWPNQKKVIAAVAAAAQGTPAP
                     PEMAQWGKTALYASRINFLLSIPMLFFMGAGSHLK"
     gene            22428..23729
                     /gene="nuoF"
                     /locus_tag="PLS35_04165"
     CDS             22428..23729
                     /gene="nuoF"
                     /locus_tag="PLS35_04165"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNI17883.1"
                     /translation="MTLPQPRVLQKLDGAPWEIDPYLKTGGYDAWRKCLKELTPADVV
                     GEIKKAGLRGRGGAGFPTGIKWDKVLNHRVPERYFVCNAGEHEPGTFKDRHLLKHIPH
                     QLIEGCLIASRTVNAKASYIYVNHEYEEEEANLRKAIAQARERGLLGKNILGTGVDLD
                     LEVFSGHGSYVAGEETAMLESMQGRPAMPRQKPPFYPTDFGLYGKPTLVNNVETLCNI
                     PQILKNGAAWFTQVGTEKCPGTMLFSLSGSINRPGVYEMPMGVTIRDLIETCGGGVPN
                     GRKIKAVFPGGPAFSMVTADQLDLPMDFDSLKKAGTGLGSAGTIVIDDATCMVAATLK
                     YSNFFKGESCGQCPPCRMGTINLATLVEKIERGEGSQKDLDSLLQLCGFVKGTGYCTL
                     ITGAAVLVQSSLRLFQHEFEEHIRMHRCPFQSAQTGVGAKA"
     gene            23742..24098
                     /locus_tag="PLS35_04170"
     CDS             23742..24098
                     /locus_tag="PLS35_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNI17884.1"
                     /translation="MPRVSFLHPEGRSGEVESNLTLLEAAKALGFGLNHDCGGNASCT
                     TCRVEVQMGGENLSEIDFDEQDLLDREALSEPWHRLGCQARVLGDVVVRVPETKWVQP
                     TSASSSQFGKKGAGLP"
     gene            24157..24483
                     /gene="erpA"
                     /locus_tag="PLS35_04175"
                     /pseudo
     CDS             24157..24483
                     /gene="erpA"
                     /locus_tag="PLS35_04175"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
     gene            24619..25068
                     /locus_tag="PLS35_04180"
     CDS             24619..25068
                     /locus_tag="PLS35_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNI17885.1"
                     /translation="MSEATITRRYRFCAAHRLHTDQLSSEENWTTFGKCNNPNGHGHN
                     YVVFVSVRGDMDSVTHQVVDLSKLDEVVQRIVVRRFDHQDLNKDPEFTTQTTTGENLV
                     LLIWNLLVNEVPGGRLVKVGVIETRDNYFEYSGSAKQVRREDGVRHG"
     gene            25061..25696
                     /gene="folE"
                     /locus_tag="PLS35_04185"
     CDS             25061..25696
                     /gene="folE"
                     /locus_tag="PLS35_04185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNI17886.1"
                     /translation="MAKVVSGSRARRSRTTATPTADDPIEGLMTKLLLELGEEPGRNG
                     LLNTPKRVAKAMRFMTQGYRQDIDHLLNGALFPIEYDEMVIVKDIDFFSMCEHHLLPF
                     FGKCHVGYLPNKKVVGLSKIPRVVDAFSRRLQVQERLTVQIAETLKKKLNAHGVAVVM
                     EARHLCMMMRGVEKQNTVAVSSSMLGVFRTQQQTREEFLKLIRGSSVKDGG"
     gene            complement(25693..26502)
                     /locus_tag="PLS35_04190"
     CDS             complement(25693..26502)
                     /locus_tag="PLS35_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI17887.1"
                     /translation="MFFQTQDIRLAYDDRGSGVPLVFLHAFPLNRSMWAPQVAALSTQ
                     FRTIALDFRGHGESDAPLWSFSLEQYADDVAALLDHLNIPQAVLVGLSMGGYVSLAFS
                     KKCGDRLCALVLADTRAQEDSPEGKRGRFHLAQTAYTQGTEAVANTMLPKLLGATSLK
                     SKPELVEQIRRTIEHTPVSGILVDLMAMAERSDSVAHLTAISCPTLVVIGDEDHTTSL
                     VDAQIMASHIPNARLAVIPAAGHLSNCEQPELFNDLLRGFVEGVRANGRRS"
     gene            26649..27968
                     /locus_tag="PLS35_04195"
     CDS             26649..27968
                     /locus_tag="PLS35_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNI17888.1"
                     /translation="MPAHDSTGDLFASSQPAPRITPSGKVPLAERLRPRSFDDLVGQD
                     DVVGPGRPLRSAIEQDRLSSVIFWGPPGCGKTTLAGLVAQYTKSQFVPFSAVTGGIPE
                     LREIIKAAEQRRTRGMATILFVDEIHRFNKAQQDAFLPHVERGTVVLIGATTENPSFE
                     LIAPLLSRSLVIVLQPLTTEALGSILDRAVADTDRGLGSLRLTIQPAARDRLIAFGNG
                     DARSLLTTLEFVAGQAPVGMDGRRIIDESVLEAALLKKALRYDKGGEEHYNLISAYIK
                     SLRDSDPDGALYWLARMLEGGENPRFIARRMVIFASEDIGNAEPQALVVANAVAQAVE
                     FVGLPEAQITLAHGTTYLASRPKDNASYVGLQEAIRDARQHGNLGVPLHLRNAVTSLM
                     KEIGYGQGYRYVHNDPAAKSEQGHLPEPLKGKRYYRPRTAQDHEERG"
     gene            28010..28387
                     /locus_tag="PLS35_04200"
     CDS             28010..28387
                     /locus_tag="PLS35_04200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17889.1"
                     /translation="MARQTNSTESVSERRKYVRATLVGSALVSPQNGAKGFTAVLNNV
                     NKIGAGLHSKETLPIGAKITVSLAFLDPDRVEQQEKLSATVAWAKPWEKGMLLGVVWD
                     DLVTKEKNRWLYYYLEETLKSTD"
     gene            complement(28384..30225)
                     /locus_tag="PLS35_04205"
     CDS             complement(28384..30225)
                     /locus_tag="PLS35_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI17890.1"
                     /translation="MLQLESVHKQYSTKVLLEGATAHLRPGARVGLVGPNGAGKTTLF
                     RMILGEESPDKGTIRKRPRLRVGYLPQELETIVGKTVLDAAHRDIYPEHEAERILSGL
                     GFTEADFSRPIENLSGGYRMRVALAHLLLSNPDVLMLDEPTNHLDKPTQRWFERFLLE
                     SNLTLLVISHDTKFLDGIATHIWELRDRTLQEYRGNYTKFQELRAARDAQIEAAANRQ
                     SKEVARVQSFIDRFRYQANKAKQVQSRIKQLDKVKLIERQRDTKRVRFKFPLPAASGR
                     HVLELKGVAKSYGEKVIYRSLNFAVERGQRIALVGENGAGKSTLLKMLAGVLPFEKGS
                     RHVGHGVTLHYFAQHQAESLNQDHTILESLAEVSAQAETNFLRGIAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPASVDMLTDAMTDFPGTIVFIS
                     HDPTFLARVATMIVEVDEGQAKTYLGDYEYFLWKKAQEFESIKESSAELAAAQNDKSS
                     GPTKAMASQVQQKSSGGERRDLNKTQARLEKQVSRAESEIASTESKVKARELELADPA
                     LYKELGRWSELQQEQETWKKELERLTARWESLSEELQGVREKLTALG"
     gene            complement(30385..30460)
                     /locus_tag="PLS35_04210"
     tRNA            complement(30385..30460)
                     /locus_tag="PLS35_04210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30425..30427),aa:Ala,seq:ggc)
BASE COUNT         6215 a         8194 c         9406 g         6556 t
ORIGIN      
        1 tccgccggtg ctctttcgaa agagggcgtc ttgtctgcga ccggacgagg cgccttacca
       61 ccctgttcct ggaatttctt gcgagaccgc cctcaagccg atagaatcct ttttatcgct
      121 tatgggtgag aatcattcag caaagagtcc cctctgcgtc gatcttgatg gcacactcat
      181 taagaccgat ttgttgtggg aatcactgct ggcccttctg aagcagaatc cgctctcgat
      241 tttcctactt cccgtttggc tactgagagg cagagcctac cttaagcatc agatcgctca
      301 aagggtcacg cttgacgcta gtacgttgcc ctatgaccag gagttggttg agtttctctc
      361 gaaagagcgc ttggccggga gagagctgat tttagctaca gccagccatg tgagttttgc
      421 acaggtggtg gctactcacc tcggattgtt tgatggacgc gtattcggca gcgacagctc
      481 cgtaaacctg aagggggctc gtaaggtcgc gctcctggtt gaacggtatg gaactcggcg
      541 gtttgcctat gccggaaact ctactgctga ttttccggtg tgggcagagg ccagtgaggc
      601 gatcgtcgtc aatgcgtcag ccggtgtagc gagtcgtgct cgaacgctga caacggtcag
      661 ccgcgttttc cgcgcgccgg tgaatttggt gaagcaagtc gccaaggcgt tgcgagtcca
      721 tcagtggtca aaaaatgtgc tggtgtttgt cccaggggtg gcatcccatc aactcgcgaa
      781 tggttggttg ttgctacagg cgagcctcgc ttttctttca ttcagtttat gtgcttccgc
      841 agtgtacatc gtcaatgatt gcctcgatct ggagtcggac cgccgacacc ctcgtaaaag
      901 aaaccggcca tttgcttctg gcaatctgtc gatcccattt ggacttgcct tagctgcagt
      961 gtgtctgatt ggcggtgtcc tgcttgcctt ggcgcttcct tcggtgtttc ttctcgtact
     1021 aggtggttac ctggctctga ctacagggta ttcgttttat ttgaagcaat tcgtgcttgt
     1081 agacgtgatt gttctggctc agctctatac ggtccgtgtt tatgggggcg gagctgcaac
     1141 tggtgtcccc ccctcccatt ggttgctgac attctcgctt ttcctctttc tgagcctggc
     1201 gctggtcaag cggttcacgg agctgcgcct catgagccaa gtcgaaggta agggtttgca
     1261 tgggcgaggg tattgggtga cggatctaga gcacatttcg agtattggaa cggccagcgg
     1321 actcttggcg gtgttggtac tggcgctcta tatcagcagc aaggaggtgc tactgctgta
     1381 ttctcatgcg gaagtcttat ggctggtgtg ccctgtgatg ttgtactgga tcagccggnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt agatgcccca gccgccgctg
     1561 agatggagat tcgccccatt gacgtagcgc gcctcttcac tcagcaagta tctcaccgcg
     1621 gcgactgtat cgttgacgct tcccacatat cccgctggga tccgtttgac cactcctgcc
     1681 agttcttccg caggggcact gcctgaatcg ataaagccgg gcgaaatcgc gttgaccgtg
     1741 atgccatagg gggcgagcag cttcgccagt gtgcgagtca ggatcaacac tccggctttg
     1801 gcgatataat gacccgtcac ttccggctgg gccaccattt ggtcggcgtt ggccatgctg
     1861 aaattgatga tccgcccatg tttcctggct ttcatgcccg gcgcgactgc ttgcccgaga
     1921 tagaaaatcg gatgaagatt tccatcgaac atctcgcgcc agcccgttgt cgtttcgtcg
     1981 aagaggttta cgcgatggta agggcctgct ccattgatca acgcgtcgat ccgtccccac
     2041 tgcgcttcga cctgtgcgac caactgcttg gaggccgccg gatcggacac gtcgcattgc
     2101 agggcaaggg cttggccgcc acgtgccatg atggccgcgc tggtctcttc tgcggctgcg
     2161 gcactcgtgc ggtagcaaat agcgatgcac caatgttgtg cggcaaggtc gagggcgatg
     2221 gcgcggccga tccccttggc tccgcccgtg atgagtgcaa cctggttcgg cataccgtct
     2281 ccttctagcc ccgccgttcg cgcaggcgct tggcgagcaa ctccagtgtt cccgccgttc
     2341 tggttgaata gatgcgttcc ttcttcggac gtttcttatc cgcgacgcag tcctcgatca
     2401 aggcttggag atcctccagt gtgtccacat cgttccagga gggcagaagt gcggtgttca
     2461 ggccttctgc ggcagcgttc tgctgagtga gtgccaggac ctgatctgtc gaccagggaa
     2521 tatgcgcaaa cagtttcggg tgcggcctgg tcatgccgat caggtagtac ccgccgtcgt
     2581 tggccggtcc caggacgaga tcatgccggg tcagtgcggc aaaggcatcg cgatagtgtg
     2641 taagcggcag cgaggggaca tcagttccca ccaggcagat gtgtcggtac ccgcgggcga
     2701 acagctgagc gaacgtgtgt tgcatgcgag cgccgaggtc gtcgccttcc tgatcgaaga
     2761 gtgtcacccc gtagcgtgct tccatgatgc ggaaaaacac gtgcgtgctc gagggcgcgc
     2821 aggccagaat acgatcgaac gacagcttga aggtgctggc cgcggccttg gtgcgttcga
     2881 gcgtgtcgag gacaaagctg ccgtgaagcg tggcggcttc gtcttcggtc aaggccggac
     2941 aaagtctggt tttcacctgt cccgggaccg gcgctttggc gaagatcacc agggcggcgg
     3001 acgcaggctg tttacggtcc gagggttgag ggctcatcac tcatctcacc atgctgtaaa
     3061 aatgttgaag tcgatgcggg ttgaccccga tccagtacag gaagcgaagg gtccacatca
     3121 acagaattgt gcgaagagga ccctgttgtt cccatcgacg gaaggcggtg acgacctcat
     3181 ccgacaagga gaccagcggc ccggttcgtt tgagccgccg cgtgaagtcg atgtcttcca
     3241 tcaggggaat gtcggcgaat cctcccatcg tctcaaaaac ggcgcgccgc acaaagatcg
     3301 cttggtcgcc ggtggcgatt ccgctccagc gcgagcgcac gttcatcaga ctgctgacga
     3361 ccagggctgt cagtcgagag gagtcaaacc ggacattgaa gcggccgccg acggcggctg
     3421 catctgagag tgcggtcaag acggcccgcc gcgcgttcga tgggaggagg gtgtcagcat
     3481 gcaaaaagag caggacatca cacgcgctcg cagccgcgcc ggtattgagc tgcctcgcac
     3541 gcccgcgtgg ggcagtcaat acacgcatcg atccgctcgc gcgtaaggga tctgtatcac
     3601 attgcttggc gaatgactgc gcaatggaga aggtgtcgtc ggaactcccg ccgtcgacga
     3661 tgatcacctc gtcaaaccct aatgcctgag tatgggcgag gatgtgagcg aggcaacggg
     3721 attcgttcag caccggcatg atgacggcga tggtcatcgg agccggagag cacccgtcgt
     3781 atgagggccg ctcgcggcca tttacgcggt cggtttgggg tccttgaaca tgaagtacat
     3841 cacgatggcg acggtggacc agaagaccac ctgcttatgc cagaacagga cctcgccgta
     3901 atgaaagaag gccagactga tcaggacgga tccggccatc aaggaatagc ggacggtcgg
     3961 attttccacg accgcgttag cccagacggc gatgccgctg aagtaaatca agagcgggac
     4021 gacgttaatt tcaaatccac cgctgatgga gagaaacacg tccaaaaaac cgatgaacat
     4081 caacgccgtg ccgatcatca tcccgaagac gtgcgcctgc cgttcggagg tgctttcatc
     4141 gtccgggcga tgtggaggcg gccaggacgg acgggtagag ggatctggga ggtcgctggg
     4201 aggtggcatg ttcaggcctt aaaatgtttg ctgagggtga gggcttgtcg ttggtatgac
     4261 gagccgagcg ctgtcccata cacttgtgat gggaccgtca gcattcgatc atacactact
     4321 ttgaagaagg tttgcccgtc atgaatcagg aatggcacat cgtgcgggcg aacttccagc
     4381 acgacctgcg tgccgcgcat cgtcccatcg ccatacccga aaccggggtc gaagaatccc
     4441 gcataatgcg tgcgtagctc accgcaggcc gcttcgtacg cgaccatttc ggcggcatac
     4501 ccggacggga cgcggattcg ttccttggag gccaggatat aaaactcttc cggctccaat
     4561 agcaggctgt cgtgccggtg ccttttgagc ggttcccaga aatccaaggc cgcatagtga
     4621 ccgatcttcg ccagatcaat gacatgactg ttcttctttg cccgatagcc gatgacggcc
     4681 tcctcgtgag cgctcccggt gaggtcgatc cgcaaaaaca gtccgcgctc cgtccggaac
     4741 tctttctgag ggagcggttt cggcgaggac gcattgcgga agagcagggg gtcggccgca
     4801 tgcaattttt ggagagcggt atccgaaacc gttgcatctc cgcgtacgaa ccggatttga
     4861 ttgaggcaat caccggtgcg caccttaatg gcaaatgacc ggggaaccac ttccagatac
     4921 aggggccctg tgtagccggc ccggatctca tcaaacccgg cgttcaagtc ggttacgaca
     4981 cgggtgaaca cgtccaggcg ccctgtcgtg cttttggggt tggcacgggc gcggagcgtg
     5041 gccggcagtt cgaggtgctc caggagcggc accagataga catggccctt ctccagaatg
     5101 gcgccttgcg tcagatccat ctcatacatg acgagatcag actgataaaa gtcgagcaca
     5161 ttgagacggc tgctgatggc cgataactcg ggcaagaagc tgctgatcag ccgataggct
     5221 tttttgccca gccgcaagtc cagactggcc ggctggatct gccggtcttc gatcggggcg
     5281 tccgacttga tggcgccacg ggtgatcagt tgggtaatgt gctggtaagg tagaatcccc
     5341 ttcgaggacg cggcccgttt cacaggtgct caccccctcc gtcacacccg ttgccggatg
     5401 cggcccagga agggtgggcg ccaaccatcg gcaactcgcg gccttgaggg gagccgggct
     5461 gaggatagtg atacgtattg ttttcgtagt ttctcaacac cgcgccgtct tcatccagat
     5521 tgaccagata gtctcccggg agtagcggaa ttttcccgcc gccgccgggg gcatcgatca
     5581 caaagtgagg cactgccatc ccgctggtat gaccttgcaa cgacttgatg atcttgagcc
     5641 ctgtttccac cgaggtccgg aaatgattcg tgcctttggt caaatccgct tgatacaggt
     5701 aatagggttt gacgcgcgcg agcagtagct ggtgcatgag ccgtttcatg acctcggggt
     5761 catcgttaac ccccttcagt aacacagtct gtgctcccag gggaaccccg gcgtccgcca
     5821 gcatgccgca cgcacgcttc acgtccggcg tcagttcatc cggatggttg aagtgcaggt
     5881 tcatatagaa gggatgatat ttcttgatga tctcgcagag tttcggggta attcgctcag
     5941 gcaggctgcc cggtacacgc gtcccgatgc ggatcaattc cagatgcgga atcgtgcgga
     6001 gcgatttgag gattcgttcc aagaggtgat ccggcagtag caatggatca ccaccggaca
     6061 ggatgacatc ccgtacctcc tgatgttccc gcagatacgc gatggccctg tcgagctccc
     6121 cctttttgag gaagcccggt ttgccgacca gccgttttcg ggtacaaaac cggcagtaaa
     6181 ttggacactg attggtgacc atcaacagca cacggtctgg ataccggtgg accagatggg
     6241 gcaccgggct catcaggtct tcttcgagcg gatcatcctc cgcatcggcg tcggccattt
     6301 cagcgcgatc cggcacgacc tgcttccaga tcgcgtcccc cttctccttg atggtggcca
     6361 acacggtcgg cgtaatgcgc atgggatagt cgcccacgat gtcttcgact tctttggggt
     6421 cgacaccgag ccgctcggcc aggtcttttg gcttcaccac gctttgagcc aggatgcgtt
     6481 tccaatcctc cacgtttcta tcctttcgct gatgccgttc caaccggccg ggccgtattg
     6541 gccggagcct tgtccgggca tctcgtctga tccgtgatcc tcaacggtcg ccctaatagc
     6601 gacctcctca cggttgcgcc ttttcgtagt gtgtctgcag gtgtgccaga atgtcgtggg
     6661 tttctgtgag caccgtgtct ccgtctttca agacggggac gtagtactgg ccggatactt
     6721 cgtagacttg tttgcgcatt ggccggaagt ccggcacgac cacgtcttcg taggacaggt
     6781 tcaattcact gagacgatcc cgaacgaccg cgcaatcggg acaccatcga acatgataga
     6841 aggtcagagc catgagttcc tattggggaa aagagtgaat agccgtccac tgaacagatt
     6901 gtattcccgt cctcacctca ccctaggcag ccacgcctgg cgcgatcgca cagggcgcca
     6961 tgattgcgtc gaccatgcca tgaatgatct tcttattggc aggacagagt gttgccagga
     7021 ttccccccag ctcggccttg tcgccggcaa caaaataata gggggataag cgaacccgcc
     7081 cggacatcgg caccacggtg tcgcttcgtt catcccagta ggacgcttcg aacaaacggc
     7141 ctttatggaa ttcttgaagc acgtaaggcg tcgtggaaaa ggactgaagc gcctgctcca
     7201 aggccgcagc ccattcgact tgcggcaggt catgcccgat ggagacgcca cggctgcccc
     7261 aggcgagttc ggagaatcct gagggcttga ccaccagctg ccgttctttt tgagtcgtgt
     7321 gggccaggcg gcgaaaatcc gatacggccc gcccgctgat gaggagatgt gggatgacgg
     7381 ctgtcggagg tacgggttgc gggtcgagaa tccaggtgcg agggaataat tgtcccaagt
     7441 tcgttctcgt ctcggcggtc aacgtctcct tccagaatgg ctccagttca gggtgatgga
     7501 atagcccgaa ggcgagcttt tcctccaagg ccggcttgta cggcggcgtg acggcgacct
     7561 gtcccttctt ggcagcatac atcatgagct cggatttcgg gatgttcttc aagtcgaaga
     7621 gctcaaaaaa gcggtagagc aatccgatgg tccgcatgcc gtgctcatcc tggagataca
     7681 gcccttcatc ggtacaacga accgctcgcg gttcgacaca aaacgcctgg aaaccgatat
     7741 cgcgcaggcg ctgggccatc cattccatct ccgggcggta ttctttcgcc tcgtcggaga
     7801 caacgatcgc caggcaccct tcccggtcgc cctgctgagc gcgcagcata gcggcaaacc
     7861 cctgcaacat gccgtcacga ccggcaatca gcggagaccg ttcggatggt ggagatgcag
     7921 gcgaagggct gaggccgagc tgatcatcat aggcgcgtgt catgcacgcc gtcaggccta
     7981 ttccgccggg cacggagtcg agctccgtga tcaccatgcc atcggccgtc gggatgacat
     8041 ccggccgaat gatgccgggg atgaggttac ggaaccggtt catccggctg aacgtgacga
     8101 gggattccgg tttgccctga tcgagatagg ccgcaaccca gccgggctgg gttccgcggg
     8161 cactttcttg ataaaggcgg tttaatgccc ggtaaaacgc cagaagatcc tgccctaagt
     8221 gctgaaaaca catgacttcc gacgaggaaa gcggaaaggg tgtcggagaa aggcggaatc
     8281 gtaccggcga aactccggaa ccggccgtgg ctgtgccctg ttccgtgtcg gccgggccat
     8341 agacgccggc gtgaacgagc gcatcacgga ttgcgagaca acgtcggcgg gcttcgtcgg
     8401 agttcaacgg ctcacgtgaa tgttgtgcca gtgccaatgc aatatcctcg atgctcgcag
     8461 cgcagtcgca tgcttcgcag tcgcggtcgg attcgaggtg gagcaaatcg taccatggcc
     8521 tcttgcactg gtacaagtcg gcgaggcgcc tgagaggctc gtcggcagct ggtggatctg
     8581 ctccacagcc cttggacggc aaacatgtgg aacagtagga ccacgtgcag gttagtgata
     8641 caatgcgcgc cagctcatga cgagtccact cgttccagtc tccccagcga atcccacacg
     8701 gcccgtaggg cagtctctcc tgcagatcgt gcccagtacg acctgtcttc attgcgacgt
     8761 ctgttgccgg tttcccgagg tagacagctt cttgcgtccc ttcttcaccg gcgatgagat
     8821 ccgtgcagca gtggcggccg gcctgacacc ggaactgttc ccgactaccg aaggcacgca
     8881 aatcgatctg gtcccgaacc cggctggcga aggatatgtc tgcccggcct ttgacagtga
     8941 gacctcgcac tgccggatct atgaggtccg ccccctggac tgccggctct atccgtttgc
     9001 gttgatgtgg gatgccgagc gcacccacgt cctcctcggg tgggatacga agtgtcccta
     9061 tatgcgcgac gctccctcgt cgctggttga ccaggcagtc gacggggtgg cgcaatgggt
     9121 tgaagcggat gagcgggtgg acacgcttgc gcgatacccc aggctcatcg gacggtatca
     9181 ggacgatgtt atccctttgc gcaccttggc acgggtgacc gaacgtgtgc agcgagcaaa
     9241 gccgcagata cgtcggcagg ccctgaccat tcaggatcga ggtcgaatag aagccgcact
     9301 gaccgtcacc gccggttttc aggagagccc gctcgccgct gcttcgttcg cctatcatta
     9361 catctggaaa catcggctga cgtatacatg ggcagagctg caggggcatc tgtgcctctt
     9421 cgccgagtcg cccgacggga tattcctggt tttgcctccg ctggggagtg ggccgatcga
     9481 tcagcccacg gccgaggcct ttcggctgat gcgggcgtgg aatggggatt cgtccgtcac
     9541 aaggatggag aatgtgccgg aatcgttcgt gcccgcattg cgtgcgtcgg gttatgagac
     9601 gggtgaaaaa gagccagact atctgtacgc tgccgcagat ctggtcgacc ttgcaggaga
     9661 agcgtaccga tcgcctcgcg cagcctgcaa ccggttcgtg cgcgaacatg gcggggttct
     9721 tgagccctat gattgtcgcg accagacggc ttgcgtgagt ctgtttcgtg aatggagaga
     9781 acagaagctg cggtccggat cggatgagtg ggccaatgct ctgctggagg atgccgtcgg
     9841 agcccatgag acagccttgt cggcacatag tgagctgggg ctgaccggag cagtggtgcg
     9901 agtcgggggg cgcattcggg cctacacgat ggggctgtgg ctcaatcaat cagtgttctg
     9961 tgtcttgctg gaagtggcgg atcgggatat tgtgggggcg ggcgcgttcg tgtttcgtga
    10021 attctgtcgc caagcgcacg ctaaaggcgc atgttggatc aatacgatgg atgattccgg
    10081 ccttccgagt ctggcgaaag ccaagcgctg gtatcatcct gcccgattgt tgccgaatta
    10141 tgttgtgacg gagttccgac gatgacggcc tcgccccttc caggtttccc tcgccgatcc
    10201 tatccgtggc ttcccgtgct gattgtgggt gtgacgattg ttgcgttggt gatcggcgtg
    10261 gtcatgttgc gctcgatcga ggtgcggatg gttgaagcca ccggagaaaa tctcacgctc
    10321 gcgtcggcgg agattgcgga caaactcgac cggctgctct tcgaacgtca tgccgatgtc
    10381 cgcatgatgg cgcgggcgtt caccgaccgc tctgccgata aaaaatatat caacgaatat
    10441 ctgcagtgga tgaaagagac ctatgcgccc gtctatctct ggctgggggt gacggatcct
    10501 cacgggcgga tgattgcggc aacggattca tccatcatcg gccaggatta cggccggagc
    10561 ggctggttcg accgggtccg gcagacccac acggtgcagg tcgatgaagt cgagatccat
    10621 gaatcaaatc ataagatcga atcggtcgcg tttaccgcgc ccattctcag tgtcagcggg
    10681 cagtttctcg gcgcagtcac cacccggatc ggcctgccga tgctggaaga tgttctcatc
    10741 gaaacggtac gcgaaattca acagaccgat ggcgcggccg gtcccttcga atatcaattc
    10801 ttgaccaagg gcggtctggt ctttgtggat tcctcttcct ccaacatcga ccgcgtcaat
    10861 ttgcgtgaac tcaatctgcc ctcagtcttg cggagcagtt ccggcaaggc gggatatgtt
    10921 gaagaaatac atgttcgccg tcatgtcccc atcgtgaccg gctattccca gacgaaggga
    10981 tacagcgatt attccggctt gcagtggacc attctcttgc ggatcgatcg agacgtcatt
    11041 ctgctgccga tccgggcgat gatgtggaaa ctcggcattg ccggagtggt cgtatggctt
    11101 ccgatggtgg gattgctgtt gtgggccacc ggacgattgc gtgagcagca tcggcaggca
    11161 cagcaagaga gcgaatgggc ccgcgcggcg gaggcggccc tcttgcagag ccaggaacgc
    11221 aaccgggtca tcgtcgatac ggcgttagac gccgtcattt ccatggatgc gggcggcatc
    11281 atcacggatt ggaatgcgca ggccgtcaac atcttcggct gggaacgcga ggaagcgctc
    11341 ggccgtcgcg tatcggagac ggtgattccc gtccgggacc gggatgggca tgagcgagga
    11401 ttgcggcatt tcctcgaaac cggacaaggc gctcttctca atcgtcgcat tgagatgatg
    11461 gggagtcatc gcgacgggca tgaattcccg gtggaaatta ccatctctcc ggccaggctg
    11521 ggcgacgcgt atattttcag cgcgttcatt cgcgatatca ccgcgcgcaa acggacggaa
    11581 cgccaactgg cttcccagta cgccgtcacg cgcgtgctgg cggaatcgcg aactttggaa
    11641 gaagctggtc caaaaattct ccaggccatc tgcgaaagcc tggaatggga attgggcata
    11701 ttctggcggc tcgaccggca gggaagtgtg ctgcgttgtt tggatgtgtg gcagtcaccg
    11761 ggactcaatg ccgaagaatt tgttctcgac acctggcggc atacgtttgc gctcggcaag
    11821 ggacttcccg gacggatttg gcaggcgggg gagccgacgt ggatcaagga cgtcgtgcag
    11881 gaagcgaatt tccctcgggg cggtgcagct tcgcgcgtgg gattacacgg cgccttcgga
    11941 ttccccgttc gagtcggaac cgaagtagaa ggcgtcatcg agctcttccg gcgcgaaatc
    12001 aaacagccgg acgagcaact cttgaagatg gtcgccgacg tcggtctcaa gatcgggcag
    12061 tttggcgaac gagcccgcgc cgaggatgcc ctgcgacgca cggaagcgca gctgcagcag
    12121 tcgcaaaaaa tggaagcggt cggacgcctc gccggaggcg tcgcgcacga tttcaataac
    12181 atgctgacag tgattcgagg ctacagcgaa ctggtgctga gtcgtctttc cccgaccgac
    12241 ggattccgga aggaattgga agaggtcaaa aaggcggcgg atcgtgcatc cggtctgacc
    12301 ggccaattgt tggccttcag tcgccggcaa tttatcgctc cgaaggtgct ggacctgaac
    12361 tcggtgatcc ataatatgga aggcatgctc cggcgcttat tgggcgaaga catcgtcgaa
    12421 ctctgtactg tgctggatcc gggattgggg cagctgaagg cggatcccgg gcaggtcgaa
    12481 caggtcatca tgaatctggc cgtgaacgcc cgcgatgcca tgccggacgg cggccggctg
    12541 accgtcgaga cggccaacgt gcaattcggc cagcgccgca accgcccgcc gatgggcgcc
    12601 gagcctggtt cgtacatcgc gctcatcgtg cgtgataccg gccacgggat ggacgaagat
    12661 acgcagtccc acgtctttga accatttttt actaccaagg aaaagggcaa ggggacggga
    12721 ctgggattgt ctacggtcta tgggattgtg aaacaaagcg gggggttcat tgaggtcgag
    12781 agcaagccgg gccgtggggc cacctttaaa atatttttcc cgcgtgttga agcggtcagt
    12841 gaggaaacgg aatcagttgc cgtgggcggt gattcggtca gagggcgcga gactgtgttg
    12901 ttggtcgagg atgaaccggg cgtgcgacgc ctggtcaatg aaacgttgcg gctccatgga
    12961 tatacagtgc tggaagccag gcatggcatc gaagcattgc tgacgggtgc caaacatgcc
    13021 ggtcccatcc atttgctcct caccgatgta gtcatgccac aaatgagcgg cccggaagtc
    13081 gccgaaaaac ttgcgacggt ccgtccggaa gtcaaagtgc tttatatgtc cggttatccg
    13141 gatcacccgg ccttctctaa gggtggcatt gataccgagc attcctttct gcagaaaccc
    13201 ttcacgccca cgacgctggc ccaaaaagtt cgtgaagtgt tggatgggtc gaaggtcagt
    13261 tgacccgagg tttctgtcgg tcgcttgaac ttcctgccgg tttccgttta tcgtggcgca
    13321 ccatgcactt tatttaattt caggcaggag tggcggaatg agcgaggggc gagcagcgac
    13381 ggcgccaaaa ggacaggcac aagacatcga ccattaccgg atcgcgcgcg agccgtttta
    13441 cgccgacgtg cggggcgagg tcgggctctt taccattgcg gcgcaaagcc gcatgccggt
    13501 gatgctcaag ggccccacgg gctgcggaaa aacgcggttc gtccagcaca tggcctatcg
    13561 gctggggcgt ccgctcatca ctgtcgcgtg ccacgaggat ttgaccgctt ccgaccttgt
    13621 cggccgctat ctcttgaagg gacaggagac ggtgtggatg gatggtccgc tgaccctggg
    13681 tgtaaagcag ggagcgattg tctatctgga tgaaatcgtc gaggctcgca aagacaccac
    13741 agtcattatc catcctctga gcgacgaccg gcgcctgctg cccattgaaa aaaaaggaca
    13801 ggtcatcgag gcggtggatg agttcatgct ggtgatctcc tataaccccg gttatcaaag
    13861 cattctgaaa gatctgaagc aaagcaccaa gcagcggttc atggccatcg agttcgatta
    13921 tccgctgccg gatatcgaga gccgggtcgt ggcgcatgaa gccggagtca gtcccgagat
    13981 tgcgcagcga ctggtcaaga ttgcggagaa ggttcgtaac ctcaaaaatc acgggcttga
    14041 agaaggcgtc agcacccgat tgttgatcta tgcggcgacc ctgatcaagc agggcgtgcc
    14101 cgcggatcgc gcgtgcgatg tcgccattgc caggccgatt accgatgatc cggacatgtt
    14161 gcgcagcatc atcgaggtcg tgaaagcgat tttctagccg ctcgtttttg cacctcaccg
    14221 actctgcgtg cttcacaatg ttgtgaccac ccacaattcc ggccgggagt ccaaatcctc
    14281 ggcagcagcg caggtcgttc gcaccacacc cgagggcgcc gttatttccg ttcatgtcca
    14341 gccgaaagct tcccggactc aatgtgcagg gcttcacggt gatgcagtca aaattcgtgt
    14401 ggctgcaccg cctgctgatg gtgcagccaa cgaagagttg tgccggttcc ttgcccgcac
    14461 atgcggcctg cccgtgagcg gcgtcgagat tctcagcggg acgagtagcc gccagaagcg
    14521 aattttactg aggggacgtt ctgccgaaca ggtgcgcgca caacttcaac ttgagtaagc
    14581 cgcggttggg tcgcacctct gaatgcatga ggcactcttg atgcgaggat gttatgcacg
    14641 gtgagccggg tgtcgagcta tggccgcggt cgtctgttct gagaaaaatc aggtccatca
    14701 tttttcgatg gtcactgtcg ctgattgtcg tggtcgtgag cggatgtgca gggcctgaac
    14761 ccatcttcaa gtcgactacc tatttgcaac tccacgggca gcaacaagcc caggatgaag
    14821 ccgcggtctg tgcggtgaag gccgagcggg ccggattgca gcatggaacg aatcgcagcg
    14881 ggaatgccgg cgccggcgcc gcgctgggcg tcatcggtgg ggcggcagta ggggcctcgg
    14941 caggactggt cggggggcca actgggattg ccatcggtgc ggcagcgggc ggcgccgtcg
    15001 gtggaatctt ggggctgttc gccggaacct acaagccgtt acacccgcga caagattatg
    15061 cggcattcgt cgaacgttgt ctgaaggaaa agggctacga gaccgagggg tggcaataat
    15121 gatcggtttt cgatcaaagg ccgtgcaggt tcagtcgtca aaatgtaaag cagcctgaat
    15181 agtcggccat caggcattga acacgaatag aaatccgttc cagccgctcc aatagttgcg
    15241 gcgtcggggc ggaaccgtgt acctctcgca gccattcaag atcctgctcc aattttctca
    15301 acgaccagcg tgatgtgcct ggaatgcgcg tctcctcgtg aagcgcctgc tccacaaggc
    15361 tggtgaacga ggcgacttca tcggcaaatt cgacgcggtg ggcatcaaaa gcatagggcg
    15421 cctcgtcctg cgacatgagc gcgagcagtt ggcgtagcca ttccaccgtc gcggccatgc
    15481 gttcgtagtg gagcgtgctg gcgacgtcgc ctgccgtgtg ataacggggt gatcgcgcag
    15541 cggagagaaa caggaatggg accttctggt tgcggaatac atcgtagtcg ctgaaggcct
    15601 tgtgaccatg gtcgggaatt tcttcgacca ggtgaattcc tccacggtag atcgtgaggg
    15661 gtgagacccg ggtttcagtg acccgtcgat acaacgcagg gcttttctct gctcccgtgg
    15721 caaaaatcgc gtccttcagc ggagcccaat gcacgcctcc catcaaatcc atgatgatcg
    15781 cggcttgaag attttccgca gcgccgactt ccggcggcag gtggtgaaac aaaaactgtg
    15841 atcccatttc ggccgttccg aagtagggaa gttcctcacc gttgaacgcg acaaacatga
    15901 tgccgtggcg tgaaagtccc gtcagatttc tggctgtctc aatcaggatg gcgatcgagg
    15961 aggcgttatc gtcggctcca agaaagggat accccagatg atcgtaatgg gccccgatca
    16021 caatccagcg ggtgcgagct gtgtgatcga tcggcgggat caccccgatg acgttgtcgc
    16081 ccatgcgatc cgaaatggtc tgccgatagc cttcaagcga ggcaagcggc tgcagtccga
    16141 cgagacgaaa ttgttcttcg atgtattggg ccgcctggcg gttgcctggc gttcccggtt
    16201 ggcgaccagc cagtgcggga ctcgcgagga aggagacgta tgactgcaat cgttgcgccg
    16261 gagctgtcgt gacgggcaac gctctcggat caagagcctc cattgtgtgg aggcagccgg
    16321 ccatactcaa agaggtcaat acggcgacaa ctcccaagcc atagcgaagg ccgactatcg
    16381 gattgcagca aaaccggctg atcacgattg tgcggaggaa tccggcgtag ccggggattc
    16441 cggcaccttc ggagccatgg gcagcgcttt gtggcgaagt gctcggcctt tgcccggagt
    16501 cggatcaggg acgaggccgt aacattggcg tccctcgtgg tcttcgggca agtactccgt
    16561 aatcggcagc ccttcttcat tgacgaagag caggcaatgg caatatttgt aaatttgcat
    16621 ttcgtcgcac gcacacatcc agcgacgcag tttggcctcg gcttgtttat cggggtagaa
    16681 attgcagggg cagaggggct ttcccaactc atcgacgtgg gcggctaacc ccaacaccac
    16741 ggcgttggtc acggcaggat tcgggtgcat ggccgtgccg ctcttctccg cgaagccttt
    16801 cacatacttc cacatcttct cgagactttc tttgctcggc tcagccattc cgtcccccgc
    16861 gtgacattgg ttcagtgaaa cagtcgatcg gtaccaggcg gtcgcctgcg acgacacagt
    16921 ttacaagcga aattgtgtcc tttacaatcc acgacttccc tcaaaatgga tcacagtaca
    16981 tggcacccgg gcactccttc ccacaactta ttgagcgatt ggccgaccaa ctcgggccgg
    17041 cttccgctac gacaatggtt gacgccatcg ttgcctcggc caccagaccg gatgcgccgg
    17101 cgggggtgtg gctgttgctg gaggagctca cagacgctgc gccgaaagcg gccaggggtg
    17161 cgatcgaggc tctgttggaa ctgcaacgcc ggggtttgct ggccgaggtg ctgccatggc
    17221 tcgacctggg cgtagccatc gcggctgatt cgggagcgtt ggcattacga tttttcaagg
    17281 agagtcccct cctgctcggt ctactcgatc cgcctcagcg agccgtggta ttggctgccg
    17341 gtttggagtt ggctgaccgc caggccaatg tggcggtgga atttattcga gttgcgccgg
    17401 aagttgtccc cgttctgcct ccagccgatt ggccgacgtg gatggaactg gcctgtgagt
    17461 tgtcagagac cgattttgcc ttggccgtgg agtatatccg tcagattcct gccatcgccc
    17521 gcgtgctctc tctggatacc gtacgagcct ggatgcagtt cgggatgaca ctcattgtcg
    17581 acaatagcct cggcaagccg gactatctgg gagcgctgga attcttccgg acgagtcccg
    17641 gcatccttgc ggatattccc gatcagatat cacgccacca agttattcgc gtgggcgcgc
    17701 agatagcaga gcggtcgccg gagatgggta ttgcccttct gtcggaagcc ccgtcgattc
    17761 tccgcggtct cccgactgat gaatggcgga ggcaggtgct gcaatacggt ctgatcctgg
    17821 cggaacgcga tgccgagaca gccttggcct atatgcgccg ttgccctgaa ttggttgcat
    17881 tgattggatc aggtgaaggg gcgcaacaga agtttcagtc ctggtttgct tccgggatgg
    17941 acgtgctgga gtacagcgtg gaagggggcc gcgcctactt tggcatggag tccgaaaaag
    18001 cactgggcgc gttgtcgcag gccatgagcg gggtgccgtt gcggcagatc gccaggcggg
    18061 tcaagttatt cgcccaggcc ctctgcggcc gcgatctacg gatttacgat ctgcccgact
    18121 cgctggaatc agcgcaaccg atgccacgtg ccacggtcag cgacgacggg cggagcatcg
    18181 ggttgccgtc gctcgtcagg cggcactcca cctatgagga gaatgtccgt ctctatatgg
    18241 tcatggccgc gcatgaggca gggcatttgg aattcggcac cttcgatctg cccctcgccc
    18301 gactggaaga tgtgagtaaa gaactgacgc aacgttatgg acgcccggcc ttacgggaag
    18361 tccgtacttt aggcgatatg tttgcacggt atcctcagcc agggctcatc cgcgatctgt
    18421 gggcgctcgt cgaagatgcc cgagtcgaat atttgctgcg tcgggaatat ccgggcctga
    18481 gccgggattt agccgtgatc gcaaaggatg cggtgcaggt tcgaagtctg gcgcatggca
    18541 tgacggtgcg cgaattggtc gtcgatcagc tcctgctcct ttccacggtc gaggccggtt
    18601 ccatgccgct tcctgatgcg ctcaagcgcg agatcatgca tctctggagc ctttgtcaaa
    18661 ccatcttcca tccgcaggcc actgcggagg aggccgtgcg attggccgac cgtctctatg
    18721 tctcgatgga tgagttattg gccatccgtc gcgaggctat tcccagtcca gatgaagcgg
    18781 aatcgcctga acaatcaatt cctgcgccga aagcctcgga ggatctttcc gacacgtatc
    18841 gtccaatgga caactgggac tatcgtggcg cgatggaccc gaatctggtt cgagaccgca
    18901 cggaatcgac acctgaacag agtcctgcgt ccggccaggg ggatagtgaa ggcggagctg
    18961 gactcgccgg tgattcaggc ggatcgtctg gtgccggtac ggcgcgttcg cagcagatgt
    19021 ctcaagagat gttggtgccg ggtcgtcgac agccctcgct ggttgaagag atgttggcgg
    19081 tggaaggtga gggcgcgcgg ccacaagatg atcagactga gacggtcaag gcggtccgct
    19141 atcgggaatg ggatgccgcc attcaggact atcggatgaa ttggtgtcgc gtggtcgaac
    19201 gggaggcggt cgaagggtcg tcggattttg tggaagggac cttggcagcc cagcgggggc
    19261 cggtcgcgct cctgcgccgc tatttcgaaa gcctgcgtcc cccgggattg cggcgtgttc
    19321 caggccaagt ggatggtgaa gacctggata tggatgccgt catcggccga ctcgccgata
    19381 tacaagcggg cattgagcca tcggaacgga tctatgtgcg gcgcgaaaag cgggaacgtg
    19441 aggtggccgc ggcgtttttg gtcgatctca gcgggtccac cagccgccag gttgaacggg
    19501 gacgacgaat cattgatatc gaaaagcagg gattggtcct cttgtgtgaa gcgctgtctg
    19561 cgatcgggga ccaatttgcc gtctacggct actccgggca gggccgccat ctggttgatt
    19621 ttgtcgtagt caaagatttt gaccaactag tgactgggcg ggcgggcgcg aagctggggg
    19681 gcatggttcc cttgcagcag aatcgcgatg gtgcggccat tcggcatgcg acgaccaagc
    19741 tcctcgctcg tcaggcaaaa actcgcttgt tggtcatgat cagcgacggc cgtccgttgg
    19801 atgacggata caaggacgag tattccttag aagatacacg catggcgctg cgggaagccc
    19861 gggcgagcgg gatcgaaccg gtctgtatca cgatcgacaa acaggcggat ccctatcttc
    19921 gacggatgta cggggatgtg cgctatgtgg tgattgatcg ggtggagggg ttgccggaga
    19981 agcttccaag gatttaccat cggctgacgg cctgaccggt tgcacatagg gccttttcag
    20041 cattggagca tacgaatcat gattgagcag tctcccagac cgaccgtgcc gccctggctg
    20101 tataaattgt tcacgggcca tcaatatccc tatgtgagac ggcaggccaa gtttgcgaat
    20161 cgggacttca agcccggaga agagcgtccg gagcctacgc gagaagcgat tgatgccaag
    20221 ttttgggaaa tctatccgcg gtgcaccgcc aaaattttgc aggaagtgaa agccggcatg
    20281 atcgtggtgt ttcacgagct gggtgaatat ccgccgggcg ggtatcagga cctggtggat
    20341 gcgccggaag aatttcttgc cgcgaagtac gggaaaaaga aaattaaagt gaatttctat
    20401 gacggcgata attttgtctg cacgatcaat ttcaaggtcg gcggctggac ggggcacgaa
    20461 ccggcgtgaa atgtgtgaag caggtgcgaa ggtgactttg cacccgagac ctcaccgttc
    20521 acgcttcacg aggtggaaat aatgggacga ccaaaaatca cagtggtggg cgcggggaat
    20581 gtcggtggaa cggccgcgca gcggttggca gagaagaatg cctatgatgt cgtgttggtc
    20641 gacatcgtgc cgggaattcc acaaggaaaa gcactggata tcactcaggc cggtcccgtc
    20701 tgcggataca gtacccgcgt ggtcgggaca aacggatatg atgagacggc cgggtcatcc
    20761 atcgcggtga ttacgtcggg gattcccaga aagccgggca tgagccggga tgaactcctg
    20821 ggcaccaatg ccaaaatcgt caagactgtg gtgcaggaat tggtgtcacg ttcaccgaac
    20881 atcattttga tcctggtgac gaatcctctg gatgccatgg tgcatgtcgc gcgaaaagtc
    20941 agcggcctgc ccaagtcgcg cgtcctcggc atggcgggcg tattggacac cgcacggcta
    21001 cggtcgtttg tcgcggagga gcttgatgtg ccggggacgg atgtgcaggc gatggtgttg
    21061 ggtggtcacg gagataccat ggtgccgctc gttcgccaaa cgaccgtggc aggtaaacct
    21121 atcacggatc gattgtcatc tgagcgtctc gcggccttga tcaagcgcac gcaggacggc
    21181 ggagccgaaa tcgtgggatt actgaaaacc ggcagcgcat tttatgcgcc gtcagcctca
    21241 gtagttgata tggttgaggc gatcatgaag gatcagaagc gtgtggttcc ctgcgcgatg
    21301 ctctgcgaag gtgaatacgg attaaaggat gtcatcgtcg gcgtgccggt caggctcggc
    21361 cgcgggggtg gggaatccat tgtggaatac gatctgacag tcgaggaacg gacggcgcta
    21421 caggcttcat ccgatgcggt gcgagacctg tgcggcgtgg ttgatcgtct gttgacggcg
    21481 taggtagtgc ccaccaagac agcgaggata gcaggttgct tgacaaggct cggcaccctg
    21541 cagtagagaa cgtggttggc agtaaaccgt tacagttggg gggcgtatga aatttctgga
    21601 ggatccgtat cagacattgg gcgcaggagt tgcgcttgct gtcggattga tagtcgtcta
    21661 tctaggtatg gccggagtgg gagcgggcga ggccgattgg ttcggtctgg ttgtccgctg
    21721 ggtccacttt ttggcgggca tcacctggat cggactgttg tatttcttta acctgatcaa
    21781 tgcgggcttt ctcaagagct tggacgggcc gacgaagaac atcgtcattc caaagctgat
    21841 gcctgccgcg ctgaactggt tccgtcatgg tgccacggtg accgtgcttg cgggcatcgt
    21901 gctctatggc tacctctatt ccaagggggg cactggggcg ttggcactcg gcatcggtgg
    21961 gctcctcggc atcatcatga tgggaaacgt ccacggtatc atttggccga atcagaagaa
    22021 ggtcattgct gcggtggccg ctgccgcaca ggggacgccg gctccgccgg agatggctca
    22081 gtggggtaaa accgcgctct atgcttcgcg gatcaatttc ctgttgtcca tcccgatgtt
    22141 gttcttcatg ggcgcaggga gccatttgaa gtaacgttac gcgtggacag aatgaagggg
    22201 gattggtcat ccgagtgggt gatcagtccc ctttttcatg cgctcgcctg tggtgtgagt
    22261 tatcagcccc tcggagcgag tctttgggct gagttgtcgt aggtctatca ggcctaactg
    22321 attgagattg aggcctaatc aggcgaacgt ggcttgacgg ctctcgaagc gcagccgtat
    22381 agtagctacc tgctaacctg ggggtgtaac ccccgaaagg gaacaacgtg accctacctc
    22441 aaccacgggt attgcagaaa cttgatgggg cgccatggga aatcgacccc tacctcaaga
    22501 ccggcggcta cgacgcatgg cgaaagtgcc tgaaggaact cacgccggct gatgtcgttg
    22561 gcgaaatcaa gaaggccggt ctgcgcgggc ggggcggagc aggatttcca accggtatca
    22621 agtgggacaa ggtcctcaac catcgcgtgc cggagcgcta ttttgtgtgc aatgccggtg
    22681 agcatgagcc gggaaccttc aaggaccgcc accttctcaa acatattccg catcagttga
    22741 ttgagggctg tctgatcgcc tcccgaacgg tcaatgcgaa ggcgtcctat atctacgtca
    22801 accatgagta cgaagaagaa gaagcgaacc tgagaaaggc cattgctcag gctagagaac
    22861 gaggtttgtt gggcaagaac attctcggca cgggtgtgga tctggatctg gaagtatttt
    22921 ccggccacgg cagttacgtg gcgggagaag aaacggcgat gttggaatcg atgcaagggc
    22981 gtccggcgat gcccaggcag aaaccaccgt tttatccgac ggatttcggc ttgtacggga
    23041 agccaacgtt ggtgaacaac gtcgaaacct tatgcaacat tccccagatt ctgaaaaatg
    23101 gtgcggcctg gttcacgcag gtggggacag agaaatgtcc cggcaccatg ctgttttcac
    23161 tgagcggttc gatcaatcgg cctggcgtct atgaaatgcc gatgggcgtc accatcaggg
    23221 atctcattga aacctgtggc gggggcgttc cgaacgggcg caagatcaag gcggttttcc
    23281 cgggaggacc tgccttctcg atggtgacgg cagaccagtt ggatctgccg atggacttcg
    23341 actcgctcaa gaaagcaggg accgggttgg ggtcggctgg gaccattgtg attgatgatg
    23401 ccacctgtat ggtagcggcg accctcaaat attccaattt cttcaaggga gaaagttgcg
    23461 gacaatgtcc tccctgccga atgggtacaa tcaatctggc gacattggtc gaaaagattg
    23521 agcgaggaga gggaagccaa aaggatctgg actctctcct gcagctgtgc gggttcgtga
    23581 agggaacggg atattgcacg ctcatcaccg gtgccgcggt tctagtgcaa agcagcctgc
    23641 ggttattcca gcatgaattt gaggaacata ttcgcatgca ccgttgtccg ttccagtcag
    23701 ctcagactgg tgttggcgcg aaggcgtaga ggagtcggcc catgccgcgc gtgagctttc
    23761 ttcatccgga agggcgtagt ggggaggttg agtcgaacct caccttgctg gaagctgcga
    23821 aggcccttgg ttttgggctc aatcacgact gcggagggaa cgcatcctgt acgacctgcc
    23881 gggttgaagt gcaaatgggc ggagagaacc tgtccgagat tgatttcgac gagcaggatc
    23941 tcctcgatcg cgaagccctc tctgagccgt ggcatcgact tggttgtcag gcgagggtgt
    24001 taggtgatgt agtggttcgc gttcccgaaa ccaagtgggt gcaacccaca tcagcgtcat
    24061 ccagccagtt cgggaaaaaa ggggccgggc ttccgtaagt tcccgcgaga tggtaaagta
    24121 acataacgtg ccgaacatgc agaccagaag gaggaccaac ggatggtaac gattacgcaa
    24181 ttggcagagc agaagataaa agaactgatg accgaagaga aagatgtggt cggcctgaga
    24241 atttatgtgc gtggcggtgg atgccacggg tatcagtatg gaatggcctt cgaatcaaaa
    24301 atggccgatg atgatacggt cattgagaaa ggtgatgtga aggtcatcat ggattctcag
    24361 agtgccccgc tgttgcaggg tgcggaagtc gattacgtcg acagtctgca agggtccggc
    24421 ttctccatta agaacccgca agccaagacc acctgtggct gcggcagttc cttcagtgca
    24481 taagagcctt ccggttgtgc ttcaaccgga acgccgatgt ttcgttgagt gacgtatcag
    24541 gccgggagtg gctcccctct ctagcgggta gctctcccgg cttgttcgtt ttgatgatct
    24601 ttctggagaa tcgtccggat gtccgaagcc accattacgc gccgctatcg tttttgcgcc
    24661 gcgcatcgct tgcacaccga tcagctgtcg tctgaagaaa attggaccac attcgggaag
    24721 tgcaacaatc ccaatgggca cgggcacaac tatgtcgtgt ttgtctcggt caggggcgac
    24781 atggattctg taacccatca ggtggttgac ctgtcaaagc ttgatgaggt ggtccagcga
    24841 attgtcgtgc gacgcttcga tcatcaggac ctgaacaaag atccggagtt cacgacacaa
    24901 acgacgaccg gagagaatct cgtcttactg atttggaatt tgttggtaaa tgaagttccg
    24961 ggggggcgtc tggtgaaagt tggtgtgatc gagacgcgag acaactattt cgaatattcc
    25021 ggatccgcga agcaggtgcg gagagaagat ggagtgaggc atggctaaag tcgtttccgg
    25081 gtcccgcgcc agacggtcgc gtaccactgc aacgcccacc gccgatgacc cgatcgaagg
    25141 gttgatgacc aagcttttgc tggaactggg ggaggagcct ggccgtaacg gtctactgaa
    25201 tacgcccaaa cgtgtcgcga aagcgatgcg gtttatgacc caaggctatc gtcaggacat
    25261 cgatcacttg ctcaatggcg ctctgtttcc catcgagtac gatgaaatgg tgatcgtgaa
    25321 ggatatcgat ttcttcagca tgtgcgagca ccatttgttg ccgtttttcg ggaagtgtca
    25381 cgtcgggtat ctgccgaaca agaaggtggt gggccttagc aagattccac gcgtggtcga
    25441 tgccttcagc cggcgcctac aagtccagga acgcttgacc gttcaaatcg ccgaaacgct
    25501 caagaaaaag ctgaatgccc atggtgtcgc cgtggtcatg gaagcgcggc atctctgcat
    25561 gatgatgcgc ggggtcgaaa agcaaaatac cgtcgccgta agcagctcta tgctgggagt
    25621 attccgcacc caacagcaga cccgagaaga attcctcaag cttattcgtg ggagcagcgt
    25681 caaagacgga ggctaggatc gcctgccgtt tgcccgcacc ccttcgacga atccccgcaa
    25741 gagatcatta aacagctcag gttgttcgca gttgctcaga tgcccggcgg ccggtatgac
    25801 ggccagccgg gcatttggta tgtggctggc cattatttga gcgtcgacga gcgatgtcgt
    25861 gtggtcttcg tctccgatca cgacaagggt tgggcaggaa atggccgtca gatgggcaac
    25921 ggaatcactc cgttccgcca tggccatcaa atcgaccaga attccgctga ccggagtgtg
    25981 ttcaattgtc cggcgaattt gctcgaccag ttcgggttta ctctttagtg acgtggcgcc
    26041 gagcaacttc ggcagcatag tattcgccac cgcctcggtg ccttgggtat aggcggtttg
    26101 cgccagatga aagcgtcctc tctttccttc cggactgtcc tcctgtgctc gagtatcggc
    26161 gagtaccaac gcacagagcc gatcgccgca tttctttgag aatgccaggc tgacgtatcc
    26221 acccattgag agtcccacca ggacagcctg cggaatgttc agatggtcga gcagggcggc
    26281 cacgtcgtcg gcatactgtt cgagtgaaaa ggaccacaga ggggcatccg actcgccatg
    26341 accgcgaaaa tcgagcgcga tagttctaaa ttgtgtggag agcgcggcga cttgcggcgc
    26401 ccacatggag cgattcaacg gaaacgcgtg cagaaacacg agtgggacgc ctgagccgcg
    26461 gtcgtcatat gccaatcgaa tatcctgtgt ctgaaaaaac atcggtcccc ctgccaagaa
    26521 ttgatgccgg atgggtggta gcattgccag ggttgagaat caaggggatg gttgagtgag
    26581 acgggccagt ttgcggtgtt tccgtggcga cgtatagaat gcagaaggcc ttgcacctga
    26641 gatcaatgat gccagctcat gattcgacag gcgatttatt cgcttcatcc cagcccgcgc
    26701 caaggataac accttccggg aaagtcccct tggcggaacg cctgcgccct cgaagtttcg
    26761 atgatctcgt gggccaggac gacgtggtag gtcctggacg tccgttgaga agtgcaattg
    26821 agcaggatcg gctttcttct gtgatcttct ggggacctcc gggttgtgga aaaaccacgt
    26881 tggccggctt ggtggctcaa tatacgaaat cgcagttcgt gccgttctct gcggtcacgg
    26941 gagggatccc ggaactacgc gagatcatca aagctgcaga gcagcggcga acgaggggga
    27001 tggcgaccat tctctttgtg gacgagatcc atcgattcaa taaggcgcag caggatgcct
    27061 ttcttccgca tgtcgaacgg ggaacggtgg tgctgatcgg cgcgactacg gagaatccgt
    27121 ctttcgagct cattgccccc ttgctttcac gatcgctcgt gatcgtgctc cagccgctaa
    27181 ccaccgaggc actcggctcg attttagatc gtgcggtggc tgataccgac cgtgggttgg
    27241 gctcgcttcg cctcacaata cagcccgctg cccgcgaccg attgattgcc ttcggaaatg
    27301 gagatgcgag aagcctctta acaacgctgg aatttgtggc cgggcaagcg ccggtcggca
    27361 tggatggccg ccggatcatc gatgaatcgg tgttggaggc cgccctgctg aaaaaggccc
    27421 ttcgctacga taagggtggc gaagagcatt acaatttgat ctccgcctac atcaagagcc
    27481 tgcgtgattc cgatccggat ggggccctct attggctcgc gcgcatgctg gagggaggag
    27541 aaaaccctcg atttatcgcg cgacgtatgg tgatttttgc atctgaagac atcggcaatg
    27601 ccgaaccgca ggcgttagtt gtggctaatg ccgtggcgca agcggtcgag tttgtcgggc
    27661 tcccggaggc acagatcacc ttggctcatg gaacgacgta cctggcatcc cggccaaagg
    27721 acaatgcgtc ttatgtggga cttcaagagg ccattcgaga tgcgcgccaa cacggcaatc
    27781 tgggcgtgcc gttgcacctt cgtaatgccg tgacgtcgct catgaaagag atcgggtatg
    27841 gccaaggata tcggtatgtg cacaatgacc ctgcggccaa atccgagcag ggccacctcc
    27901 ctgaaccgct caagggcaag cggtattatc gtccccggac ggcccaagat catgaagaaa
    27961 ggggctagac aaagctggtt atacggttat acttagcacc gaggtcgcta tggcgcgtca
    28021 gaccaattca accgaaagtg tcagtgaacg ccggaagtat gtccgggcca ctctggtcgg
    28081 gtcagccctg gtctccccac agaacggggc caagggattt acggcggttt tgaataatgt
    28141 gaacaagatc ggcgcgggat tgcattcgaa ggaaaccctg ccgattggtg ccaagatcac
    28201 ggtctccttg gcatttctgg atcccgatcg cgttgagcag caggagaagt tgagcgccac
    28261 ggtggcctgg gccaaaccgt gggagaaggg gatgttgctt ggcgtggtgt gggatgatct
    28321 ggtgacgaaa gaaaagaacc gctggttgta ttactatctg gaagagacgc tgaagtcgac
    28381 ggactaaccg agtgccgtca gtttttcccg taccccctgc aattcctccg acaacgattc
    28441 ccagcgggcc gtgagccgtt ccagctcttt cttccaggtc tcctgctctt gttgcaattc
    28501 gctccagcgg ccaagttcct tatagagcgc cggatccgcg agttccagct ctctcgcctt
    28561 gaccttggat tccgtagagg cgatctcaga ttccgcgcgc gagacttgtt tctcgaggcg
    28621 tgcctgggtt ttgttcaagt cccgccgttc tccgccggaa gacttctgtt ggacctgcga
    28681 cgccatggcc ttcgtgggac ctgacgattt gtcattctgg gcggcggcaa gttccgcgct
    28741 gctttccttg atcgattcga attcctgcgc cttcttccac aagaaatact cgtagtcgcc
    28801 caaataggtt ttggcctggc cttcatccac ctcgacgatc atggtcgcaa cgcgggcgag
    28861 aaacgttgga tcgtgagaaa tgaacacgat tgtccccgga aaatcggtca tagcatccgt
    28921 caacatatcc accgaggccg gatcgaggtg gttggtcggc tcatcaagca aaagggtatt
    28981 ggccggttcc accagcatgc gagccagggc cacccgattg cgctcgccgc cgctgagtgc
    29041 cttgatcggc tttttctgat cgtcgccgct aaaaaggaag gctccggcga tgcctcgaag
    29101 aaaattcgtt tcagcctgcg ccgagacttc ggccagtgat tccagaatcg tgtggtcctg
    29161 gttcaaggat tcggcctggt gctgagcaaa gtagtgcagg gtcactccat ggccgacatg
    29221 gcgggatccc ttctcgaacg gcaatacgcc agccagcatc ttgagcagcg tacttttgcc
    29281 ggcgccgttt tcacccacca gcgcgatgcg ctggccgcgt tccaccgcga aattcaggga
    29341 tcggtaaatg accttctctc cataactctt cgcgacgccc ttgagctcga gcacgtgccg
    29401 gccgctggcc gcgggcagcg gaaacttgaa ccgcacccgc ttggtatcgc gttgccgttc
    29461 gatcagcttg accttatcca attgcttgat gcgcgactgc acctgcttgg ctttgttggc
    29521 ctgatagcgg aagcggtcga taaagctctg cacgcgcgcc acttccttgc tttgccgatt
    29581 cgccgccgcc tcaatctgag catcacgggc cgctcggagc tcttggaact tcgtatagtt
    29641 cccacgatat tcctgaaggg tccgatcccg gagttcccag atatgcgtcg cgatgccatc
    29701 caggaatttg gtgtcgtggc tgatcacgag cagcgtcagg ttggattcca gcagaaaccg
    29761 ttcgaaccac cgctgagtcg gtttgtccaa gtggttcgtc ggctcgtcca acatgagcac
    29821 gtcggggttc gagagcagga gatgcgcgag cgccacgcgc atgcggtatc cgccggagag
    29881 gttctcaatc ggcctggaga agtccgcttc ggtaaaccct aacccggaaa gaatacgttc
    29941 ggcttcatgt tccgggtaga tgtcgcgatg ggcagcatcc aacacagttt tcccgacgat
    30001 agtttccaat tcctggggga ggtagccgac gcggagtcgc gggcgtttgc ggatggtgcc
    30061 tttatcggga gactcttcgc ccagaatcat ccgaaagagg gtcgtttttc ccgcgccatt
    30121 ggggcccacg agaccgacgc gggctccagg tcgcagatgg gcggtagccc cttcgagcaa
    30181 cactttcgtg gaatattgct tatggacaga ttcaagctga agcatggatt agtagtactc
    30241 ggggtctgag gtgaaacgtt cggcgaggta cccgctgcgg agtgtcagcg cccccgtgtg
    30301 aagcggcggc tggctaagcg tatgactcgt agagttcgga tatccccgca cacatcacgg
    30361 agtgttggtc ggacaagctt gttttggtgg agctggccgg gattgaaccg gcgacctcgt
    30421 gaatgccatt cacgcgcgct cccaactgcg ccacagcccc acgcaagctt g
//
LOCUS       DAOQEW010000022        28370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_375_length_28370_cov_7.545047, whole genome shotgun sequence.
ACCESSION   DAOQEW010000022 DAOQEW010000000
VERSION     DAOQEW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_28370_cov_7.545047"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..582)
                     /locus_tag="PLS35_04215"
     CDS             complement(109..582)
                     /locus_tag="PLS35_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17891.1"
                     /translation="MNLRFAFIILVLAFIALLLAIGVLTAIRWLRIHYPERANAILAA
                     SAVIVAGAVIMTIIEMNDRPMFRPHDLITLQEPVVARTIPGDRGAGSATCVIDLHEHL
                     GVLEIEIERGALKARVESNNTSAPVFCPIGTEVRIDLNWLHRLTITRRQTQVSGS"
     gene            complement(579..2141)
                     /locus_tag="PLS35_04220"
     CDS             complement(579..2141)
                     /locus_tag="PLS35_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI17892.1"
                     /translation="MLRTQPSALKVPTPAILIVDDDPDIALVLKDLLEHSGYQVQVAV
                     TGAEALTRAKTDSFSAVLLDLMLPDMDGLSVLTLLKGIDPALPVIMLTAFVEVAKKHA
                     SLTEGAFGYLTKPYDGEELKALIKRAVGVKHLSLEAASAKQALTASEDRFREVVQTAP
                     DAIVLADGEGRILSWNSAAERLFGYRAEEVLDRSLTMIMPERYRDNHERALERVRTTG
                     DVRLKGTVVTMHGLHKDGREFPIEMSLSSWISSGTRVHCGIVRDITARKEAEARLLQQ
                     QIEQQALLDLIPAMVWYKDSHNRILRANRRAAESIGKTVADIEGRSTDEFYPEDAERY
                     HQDDLEVIVSGRPKLGIIEPHRAGGKKHWVQTDKVPYLDPAGKVIGVLVFAQDITDQK
                     RHEAALRENQDLVRIVMEATGDGIVIVDGDGNILAVNTRVAELFGYASGDLTGQSLQR
                     LIPSLFNFGTFELASDRGASTKGESLPLNPTCVGLHKDGAEIAVSISSTMLMTANGAH
                     GVVAVHPLGGPG"
     gene            complement(2330..2506)
                     /locus_tag="PLS35_04225"
     CDS             complement(2330..2506)
                     /locus_tag="PLS35_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17893.1"
                     /translation="MKNVEMSVEGTWLTIKVDLSKEFGPSASGKTTIIASTEGNVTIT
                     GREEKIGLNVYRKK"
     gene            complement(2516..2719)
                     /locus_tag="PLS35_04230"
     CDS             complement(2516..2719)
                     /locus_tag="PLS35_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNI17894.1"
                     /translation="MSVARVTEIIAASPKSFDDAIHQGIARANKTLQHVKSAWIEDQK
                     VDIEDGKITQYRVALKVTFVLNE"
     gene            2967..3677
                     /locus_tag="PLS35_04235"
     CDS             2967..3677
                     /locus_tag="PLS35_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HNI17895.1"
                     /translation="MPTEIEIQITPLFSTPLLVFTPADHTRINARLSTMILEREASVP
                     AHRDNEVVGWSSPHDLSMLEWAGEPLRDLFAPVLEVAKQVTTLSQRCGQGTGRPEWDV
                     VEVWANVQRNGGTNAAHSHPGSFWAGVYYVDVGEVSSNKDMGGELQIYDPRGCLPRML
                     APYLQYSMTELHDAGTSISYAPTSGQCVLFPGWLFHAVNTYHGKAPRISVAFNLDPVL
                     APGMMNGTQKRPITESRR"
     gene            complement(3702..4304)
                     /locus_tag="PLS35_04240"
     CDS             complement(3702..4304)
                     /locus_tag="PLS35_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17896.1"
                     /translation="MTGPRRSTLFSFRRSGGRPIVGSLALLAVLAGGCSPYEWRHENQ
                     WESRDQIWLSEASQVKVRAAQSRVFDGADRVKTIQAVVSTCQDLGFAVEVLDEALGIV
                     SAKKFDDYEPTRLRDPSYHLYRDDGLLILTPHFRSWGPFYHRSNLVRLTVTVRPRGEA
                     QLVVRASAQYYLKAIESPEPYQQFFRTLEQALFIQASTLP"
     gene            complement(4308..4745)
                     /locus_tag="PLS35_04245"
     CDS             complement(4308..4745)
                     /locus_tag="PLS35_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17897.1"
                     /translation="MNRTQFIGAVGIVAAALALHGCVAPEPRQELFAPTDAQMKIRSA
                     QSRTFEVTDSAVAMRGVIAALQDLGFIIERANAPLGLVTAARFAEPNYYDVLGVTVTV
                     RPQEAGKTMIRANAIYNSKPIEDPKVYQNFFAAVERSLFLVKD"
     gene            complement(4758..5312)
                     /locus_tag="PLS35_04250"
     CDS             complement(4758..5312)
                     /locus_tag="PLS35_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17898.1"
                     /translation="MILVLACGLFLGGCAEQTQPAQFFELSPESSANRAMQTRLFETG
                     ADNELLSASAASLQDLGFQIEESVREVGLLRAAKERSAREYGQYRNRFLIWLVSLGHL
                     VIPVDLHQKIAATIVIRPANHSDARQEVRIIFYRVIWKGDGQADRNYIPPGEQKMEMI
                     RDPEIYQQFFAKLSKAVFLEPHSI"
     gene            complement(5350..5751)
                     /gene="bamE"
                     /locus_tag="PLS35_04255"
     CDS             complement(5350..5751)
                     /gene="bamE"
                     /locus_tag="PLS35_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNI17899.1"
                     /translation="MRHMQWGLALAGICVVIGLTGCGGKQAEIKEDRLTVGRVQGEVK
                     VGMSAAQVAELLGSPNIVTTDDKRREVWIYDKVSTDRVDTSSSSYAGLIILGANSSDH
                     SSSQRQRTLTIIIKYDEEKKVRDFAYNSTQF"
     gene            5939..6451
                     /locus_tag="PLS35_04260"
     CDS             5939..6451
                     /locus_tag="PLS35_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNI17900.1"
                     /translation="MPMPDAMRTEEVQAACDALVARVRTAVLLTLKQGHPFGSHVPYL
                     MGDDWSRVYLHLSGLASHTQHMLKDPRISLFIAEPDGAGKNPSALQRLNLQGSGAILS
                     PSHQSYDKVKERYLGQFPQSRMLFGFGDFAMWELHMSDAHLVLGFGHAYQAHSDNPRE
                     WQHQKPETKP"
     gene            6558..7067
                     /locus_tag="PLS35_04265"
     CDS             6558..7067
                     /locus_tag="PLS35_04265"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNI17901.1"
                     /translation="MVRSASCTVLCAESRELTGVVPYMVFAAPIILSYMLLLVGLAVA
                     APGVGWGGSLIEFVPEAWRDGAHVPAYGVFAWLVMWGLLRRGWPVTHATSIGILATGV
                     FGLWTEVAQGTAPGREVSLHDVWSDLLGSTIAAAFMLWREKAIRMSKGFMVGHKRSHH
                     HLMKGTRSR"
     gene            7064..7405
                     /locus_tag="PLS35_04270"
     CDS             7064..7405
                     /locus_tag="PLS35_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI17902.1"
                     /translation="MNASYRQRGTRRIPVQYPIYYSNGAFQTIGVTEDFNIEGGRVRG
                     REAVTVGMELVVIVIQAIPETPMLIRRATVRWSKGHDFGIALSGVPPQSEAELKAMAK
                     VMLPGLWSCLN"
     gene            complement(7409..7531)
                     /locus_tag="PLS35_04275"
     CDS             complement(7409..7531)
                     /locus_tag="PLS35_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17903.1"
                     /translation="MSWENWAVAIGLGALLMMFFGLMEMFFAGPRHPLDPSHRK"
     gene            complement(7586..8533)
                     /locus_tag="PLS35_04280"
     CDS             complement(7586..8533)
                     /locus_tag="PLS35_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17904.1"
                     /translation="MGRKYDLVDLMAAIGLVATLFGGYLLVTAADGFWQAPITPTMSS
                     VIASNEPSTGMQYLQPVLGQAIVQDLLLERDAGAALSTSAMELNRAVSESERISTTLL
                     SPLVLAELRAFGQDTDHRARMQYVMGKSVVNQTRRGVSSGMLSPDQYGGEFNTNLIRT
                     AELTGQRMHDQFEATRQPALGQSIIEAIQEEDRMTATAQERVGRAVVQVTQAQEHYAE
                     AKADRQIQLASATVAAVRTDALMDRLAILENMSADRTMIAHHRAASADVSRGSLILAC
                     VGLIALFVGGLAFSTRKGEVDSIPLWKLETLLQLHRSGR"
     gene            9031..9429
                     /locus_tag="PLS35_04285"
     CDS             9031..9429
                     /locus_tag="PLS35_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17905.1"
                     /translation="MAIITTILLFVMWALISPRCDADAQSLPFRESHGTTGTLSPLGD
                     GSAIYSDPHGNTNPTQLGSGVPSHSFSGPHGATPGTLTPFGTPTPPNLLTPAPLLPLQ
                     PKGMAIPQPQSPASPSPPGRFAPPGGRPGR"
     gene            9526..11160
                     /gene="lnt"
                     /locus_tag="PLS35_04290"
     CDS             9526..11160
                     /gene="lnt"
                     /locus_tag="PLS35_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNI17906.1"
                     /translation="MTISRARLILLACASGFLYPLSFPAFDLGIVAWFALVPLHIAVE
                     YASPRRAFRVGWLAGTLAFTGAMFWVITAMNVYGKVPLPIAVLVMLLLTVYLGLYLAV
                     YAGALSWIRIAFPTLGFLPAPFLWVTLELIRTYFLSGLPWALFGYSQYQWLAIIQFAD
                     HFGVYGVSFLLVLVNAALAETLIWSFKAYRGFQIRSVPWPSLVAAASGFGVALFYGTS
                     VLSAAQHATPRMLSVGLVQPNVEQAQKWDVAFRQETMSRYDRLTTGLGENLDLIIWPE
                     AATPFVFEMEPQYRAIVNDMARRADAPLLFGSPALRRYPDGKPFLLNSAYLLATDGQI
                     LGRYDKQHLVPFGEYIPFHNSLLFFLDKLVEGIGDFEAGPGSTLLMFKPHEKTPPMTA
                     AASNADFHLKFGVVICYEVIFPNLVREFAANGADFMVTVTNDAWFGPSSAPYQHFGMV
                     VFRAVENHVAFARAANTGISGFIDPYGRVLQQSPIFTQEALRGTIPVSHQQTFYSQYG
                     DLFAYACAILIALLCLTGYFSPNAEPAGGEPAGRPA"
     gene            11179..12322
                     /gene="prfB"
                     /locus_tag="PLS35_04295"
     CDS             join(11179..11247,11249..12322)
                     /gene="prfB"
                     /locus_tag="PLS35_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNI17907.1"
                     /translation="MLDDVRTRVRTLGEQLAQLRGHLDLARMTAELNDIDVKTSQPDF
                     WKDTQAAAKVNRRKAALDRELSRWRDTERRQSDIEALLELALESGDAALEQELTAELE
                     PFEKAVAQFRIELLLSGELDANNAIFAIHPGAGGTESQDWAQMLLRMYVRWAEQKGFK
                     IETLDLQAGEEAGIKSATLSVNGPYAYGYLKAEAGVHRLVRISPFDANKRRHTSFASV
                     FVYPELDDDVEVVIEDKDLRVDTFRAGGAGGQNVNKVETAIRITHIPTGIVVQCQNER
                     SQLQNRNGAMKILRARLFEVEQRKKEAEFNAIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTGHEVGQVSAVMDGDLDGFIEAYLQRKMTGGKTPQPVAMTDDDV"
     gene            12397..13878
                     /gene="lysS"
                     /locus_tag="PLS35_04300"
     CDS             12397..13878
                     /gene="lysS"
                     /locus_tag="PLS35_04300"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNI17908.1"
                     /translation="MDELNDQRQQRIKKLDQLRQLGVAPYGTRFEIKDRAGQLVQLHG
                     EKSKEVLEQEQVGCTIAGRIVGLRRFGKAAFAVLQDGSDRLQVYLKKDTLGDQAHQIS
                     EGLDLGDWIGVTGILFRTKTNEFTVEAKTLTFLSKALRPLPEKWHGLTDVETRYRQRY
                     VDLIANPQVHQIFALRSRIISGIRAFLIERGFLEVETPMMHPIPGGAAAKPFVTHHNA
                     LDAELYLRIAPELYLKRLIVGGFPRVFEINRNFRNEGISTIHNPEFTMLEFYVAYADY
                     QDLIILTEQLFSRLAQDILGTMTIDYQGAQINLGSPWRRWSYHQAILEVNSLPPSVLT
                     NRDEALAAAKRLGVEVSPKESLVNILNEIFEETVEPKLEQPTFITDYPIEISPLARRK
                     DSDPSLTDRFELYIAGREIANAFSELNDPLDQRERFEAQAAQHAAGDEEAHRVDEDFL
                     RALEYGMPPTAGQGIGIDRLVMLFTNQASIRDVVLFPQLRPEK"
     gene            13881..15158
                     /locus_tag="PLS35_04305"
     CDS             13881..15158
                     /locus_tag="PLS35_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17909.1"
                     /translation="MALPYEIFIGLRYLRAKRRNRTISFNTIVSIVGITLGVAALIGT
                     VGIMTGFKEDVQAKILGTTAHILVNDRMKESMVDYEEQVKKVAGVPEVLAATPFIFRQ
                     VLLTSPSGVQGIILRGIDPAREGTVTELAHNLVNGNLDDLSHSTKVVIPEKEREPNGP
                     DTAMRPGIILGKELSMRLGAFPGDTLNVVSPVGPVTGASMTPKIRQFVVVGIFQSGMY
                     EYDSSLAYIELGEAQKFFNMGATVTGIEVKVADVFRAADVARSIEQQLGFAFWARDWM
                     QMNRNLFSALKLEKTMMFLLLVLITIVASFNIVSTLTMIVTEKQREIAILKAMGATRK
                     GIMRIFMLNGLIIGCSGAAIGVPLGYAFLWLIQTFWTFDPTVYYISRIPVHVLGSDVL
                     LVAGSAILISFAATLYPSLQAAKLDPAAALRYE"
     gene            15231..15899
                     /locus_tag="PLS35_04310"
     CDS             15231..15899
                     /locus_tag="PLS35_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17910.1"
                     /translation="MIDIVDLYKTFPMGGRELVVLNNINLRIQRGELIAIMGASGAGK
                     STLLQILGTLDRPTKGTVSFDGQNLFQLTEQQQAEFRNKRVGFVFQFHHLLPEFTALE
                     NACLPAMIQKREMADVVGEATKLLGEVGLADRLHHKPGELSGGEQQRVSVARALMQQP
                     DLVLADEPTGNLDSHTGDALFALMRQLNRSRGTTFVIVTHNEKLSAQADRIISMQDGT
                     IISS"
     gene            16007..17152
                     /gene="traF"
                     /locus_tag="PLS35_04315"
     CDS             16007..17152
                     /gene="traF"
                     /locus_tag="PLS35_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNI17911.1"
                     /translation="MNRQVMSYVQYTAVLLAVLLPLQAAAVEFVFVGSRQMGMGGAGV
                     ATTTDSLATYWNPAGMAMSKKFDMRIQGGGQVVDRGDVFDTIKDINHINLNDTSAPNI
                     ARLQQQIDRMNKPGTNLTAAASGGFYVKGNFGEHALGFNVSDVATSGGFLRGPVGFTN
                     NGTNLTVNGQFAMNGLEVRQAALSYAYAFMDRMFSIGVTGKVIQGAAYTGATNIRGAS
                     DDVKVFEDIGRAKISSALGIDVGAMFRPSSWLRMGIVAKDINAPTFDAPNGDKFKLLP
                     QVRTGIAVNPYNSLTLTADVDITKNNTLTPGVYSQVLSLGAEQTILSELLSFRLGAFK
                     NMQDAKTPFIPTAGFGIRILALRIDIAGGYDFRDQAALASGSIAVTF"
     gene            17199..17804
                     /locus_tag="PLS35_04320"
     CDS             17199..17804
                     /locus_tag="PLS35_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17912.1"
                     /translation="MTRHTFSTISVVLCLVMTGLLAGCGGLQEMWEGPAARGFYPKTM
                     AILPISGTYDSAREDVEEVVAKVLIKSRRVEKVVPPDQVTDTFQTTKDAFDALVTYFS
                     RMETTGQSDKNSAIKIGHALGADSLLVVKVNAWEYTREEGDNIARVGLSMRLVDAVNG
                     AIVWKARHQVQESYLFIRPDMKDLATKLAADMIKYMPPEKR"
     gene            complement(17820..18875)
                     /locus_tag="PLS35_04325"
     CDS             complement(17820..18875)
                     /locus_tag="PLS35_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNI17913.1"
                     /translation="MAVARRKISRTTRKTTSTNSSVAARRKTTVRKPAPIGEDRLLRT
                     LQQGLFNTLSSAEGEGRSPIGVAAVFVQLFLQLTKVQAVALYVRDEQTREMACLAEAG
                     SSDPSVTELWLEGLKKAEQNAQIVSGELHGVRLHRINRLDGVVMFRAGKSQRLPSRKL
                     LSLLTAVEPWLAVLLDHARLTVKYAAKILRIQHMEQVSDILNSSLAEEEKLRRALDAA
                     VRLVEAEAGALFLASPDGTLSLSALGGERAAGLTALHSTVAAGVHRTGQAVLITQGRQ
                     DPRLVAGQGWQAALSVASLVSVPVRMGARAVGVLEVVNRRSGKPFSNWDVLELASLSN
                     QFGLAIDNLRRGSGVRA"
     gene            complement(19076..19876)
                     /gene="trpA"
                     /locus_tag="PLS35_04330"
     CDS             complement(19076..19876)
                     /gene="trpA"
                     /locus_tag="PLS35_04330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNI17914.1"
                     /translation="MNRLDQTFGRLKAKGEKALITYIMAGDPSLQDTEQLVLELERAG
                     ADIIELGVPFSDPIADGPVIQQAAERGLQSGTSLRRILEAVTRLRDMTQIPIVLMAYY
                     NNIHAFGEADFCSKAAAAGVDGLIVPDMPPDEAGPLREPAAAADLHLIFLLAPTSTAS
                     RRAYVAKESGGFVYYVSITGITGAKLNDMAGVRDNVAKIRKHTKTPVAVGFGVATPED
                     AARVAETADGVIVGSAIVRRVGEHGQDGRLVHEVGSFVRSLKAAMQPG"
     gene            complement(20041..21231)
                     /gene="trpB"
                     /locus_tag="PLS35_04335"
     CDS             complement(20041..21231)
                     /gene="trpB"
                     /locus_tag="PLS35_04335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNI17915.1"
                     /translation="MAIPDRHGRFGPYGGRYVPETLMPALLELEAAYQRTRKDKSFQA
                     ELKHYLTEYVGRPTPLYFAQRLTKRLGGAKIYLKREDLCHTGAHKINNAIGQALLARR
                     MKKPRLIAETGAGQHGVATATVAAMFGLECEIYMGTEDMQRQALNVFRMRLLGSKVTG
                     VDAGSRTLKDAISEAMRDWTTNVRTTHYVLGSVLGAHPYPMMIRDFQAVIGREARKQI
                     LSAEGRLPDCLIACVGGGSNAMGLFHAFVPDKKVRMVGVEAAGLGVESGKHAARFAGG
                     RPGVLQGTMTYLLQDENGQVNLTHSVSAGLDYAAVGPEHSYHKETNRAEYTSATDDEA
                     LAAFDLLAREEGIMPALESAHAIAEVVKCAPRMKKNQIIVVNLSGRGDKDVQQVARIK
                     GVTL"
     gene            complement(21320..21952)
                     /locus_tag="PLS35_04340"
     CDS             complement(21320..21952)
                     /locus_tag="PLS35_04340"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNI17916.1"
                     /translation="MTVKVKICGLTNAEDAAVAVEAGADAVGFVFHKKSPRCAETEAV
                     KAIVKELPPFVLPIGVFVNEEAKVVRDIMDSCGLALAQLHGDETAAYCESLGRPVLKA
                     IRLRDRRSFLALAEFQGRAGVRGFLVDAFSPDAYGGTGQVADWSLAAEAASVARILLA
                     GGLTPDNVIQAIEQVHPYGVDVSSGVEAKPGKKDHAKVRAFLEAVKRLPR"
     gene            complement(21956..22270)
                     /locus_tag="PLS35_04345"
     CDS             complement(21956..22270)
                     /locus_tag="PLS35_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17917.1"
                     /translation="MRTLLLFGLMAMGLTVAGCVNQEIHEYSPKWDSWMGSTKDDRIK
                     EMGIPTRCHSFKDGGEVCEWMIPQQDGRQDLIGLTFNPKGQACQWSYRGFYGMQKSQT
                     SC"
     gene            complement(22267..23091)
                     /gene="trpC"
                     /locus_tag="PLS35_04350"
     CDS             complement(22267..23091)
                     /gene="trpC"
                     /locus_tag="PLS35_04350"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNI17918.1"
                     /translation="MILDRILEHKKAELRHKSSRGYLADLKTRIRDAGPTLGFAVTLD
                     ARRTADRPALIAEVKKASPSLGLLRPEFEQRFEPVKIAEAYREHGASAVSVLTDKDFF
                     QGDLAYLADVKRTVGLPALNKEFMVADIQFYEARAYGADAVLLIVAGLEKRQLIDFSA
                     LAKELSLDVLVETHHERELDTVLEWLPDVRLIGINNRDLKTFSTDLGVTLRLAKRIPA
                     DKMIVSESGIHTRKDVERLVEAGVHAMLIGESLIRAQDTGAKIRELLGDPPTKEQS"
     gene            complement(23096..24112)
                     /gene="trpD"
                     /locus_tag="PLS35_04355"
     CDS             complement(23096..24112)
                     /gene="trpD"
                     /locus_tag="PLS35_04355"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNI17919.1"
                     /translation="MIKDAINKLAERADLTEQEAETVMGEIMDGSATCAQIAAYLMGL
                     RMKGETVEEIAGSVLAMRARATHIRVKDSLVLDTCGTGGDRAHTFNISTTAAFVVAGA
                     GITVAKHGNRSVSSKSGSADVLAALGVAIQLPPEQVADCVNEVGIGFLFAPLYHSAMK
                     HCAQPRQELGIRTLLNILGPLTNPAGARLQVVGVFDAGLTELLAKVLVHLGAQHCFVV
                     HGMDGLDEITVTDRTRISEGKAGVVSSYTIDPAEFGLARVRPKDLVGGNAEDNAAITR
                     DIFRGRKGPKRDIVCLNAASALVAGRKAKTLQEGFELAQRTIDAGAAMEKLEQLIAFT
                     KKAS"
     gene            complement(24152..24715)
                     /gene="pabA"
                     /locus_tag="PLS35_04360"
     CDS             complement(24152..24715)
                     /gene="pabA"
                     /locus_tag="PLS35_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNI17920.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEDVVVYRNDKISIPEMESLKP
                     SRLVISPGPCTPSEAGVSVEAIKHFGGKLPLLGVCLGHQSLAVAFGGEVIRAERLMHG
                     KTSMVRHDGRTLFRGLPNPFEATRYHSLIVNRKNLPDCFEISAETAEGEIMGMRHKTL
                     GIEGVQFHPESILTTAGKELLRNFLKL"
     gene            complement(24763..26259)
                     /gene="trpE"
                     /locus_tag="PLS35_04365"
     CDS             complement(24763..26259)
                     /gene="trpE"
                     /locus_tag="PLS35_04365"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNI17921.1"
                     /translation="MTKQQYSLTLDEFRGLATEGNLIPLYREILADYETPVSAFAKID
                     QGPTAYLLESVAGGENWARYSFLGSGSSAVIHEEKGDLILTRGKKRLKIQSRGNPLER
                     VRELMEEYRPVTVPGLPRFVGGAVGYLSYDVVRTFEELPSLRKDSLGMPEVAFLLTDT
                     LLIFDNVSQKIKVVANAYLESTTDRAVREAYRHATARIDKMIARLKRPVRQPRVKRRR
                     KPITFTPNMNRADFEKMVTRTKEYIRAGDIVQAVLSQRWETQIHTTPFQLYRALRVVN
                     PSPYMYYLRVAGVELVGSSPETLVRCEDGQISLRPIAGTRRRGNSPDEDQDLARSLLA
                     DEKERAEHVMLVDLGRNDVGRVAARGSVKVDSLMQVERYSHVMHIVSQVTGQLEKGKS
                     VYDVTRACFPAGTVSGAPKIRAMQIIEELEPTRRGPYAGAVGYFGFSGNMDMCINIRT
                     VVIKGRQAYIQAGAGIVADSVPEHEYEETCNKARAMMKAIELAEQGLE"
     gene            complement(26281..26967)
                     /locus_tag="PLS35_04370"
     CDS             complement(26281..26967)
                     /locus_tag="PLS35_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNI17922.1"
                     /translation="MMSEQQGRLRRRSVVPIGLLILGSLGLGGCVMSEKYEAEKARSL
                     NFQRLLAQEEKRSAELDAEVKRGKRELSEYEARNRELGAQVEAVRQQAAQIQEEAQAM
                     KEASLLEKRAHEDMKRLTTIPKAKKAAPKKDFAAAVDEALKTEVPSDLSLEPATGAAK
                     DALTHSDLMDGAGATAAAATGTTHTVRPGETLYRIGQKYHVAPDQLRKWNNLKDNTVG
                     VGQKLIVSQP"
     gene            27203..28156
                     /locus_tag="PLS35_04375"
     CDS             27203..28156
                     /locus_tag="PLS35_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI17923.1"
                     /translation="MASGAQPASSRVISIEGITLHLAQPMSMGQEWIGSREILKQLLA
                     CWLVIDERDLPLSPRITGQPGIGKTTLAMAGARERKQDLYVFQCTADTRPEDLLITPV
                     LAESGTISYHASPLVTAVLTGSICVLDEGNRMNEKSWASLAPLLDHRRCVESIIAGIL
                     IKAHADFRCCVTMNEDASTYEVPDYILSRLQPTLGMGFPAREDELAILRYHLPFAPAE
                     MLALTVEFLQEAHQLSLEYSVRDGIHLLQYALKRRAQDPTDPLSVDAAWRESLIKVLG
                     EEALDLPMQSRRRKRALGDQALPQGLGDFFFGSDDPLHPGR"
     gene            28156..>28370
                     /locus_tag="PLS35_04380"
     CDS             28156..>28370
                     /locus_tag="PLS35_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17924.1"
                     /translation="MAAPSSTADDVFRLSPRIELFPVLHGSGDVAQEVRDRLTDRRYD
                     CLAVPLPPSFEHPLEEAVVDLPTISVIV"
BASE COUNT         5733 a         8855 c         7848 g         5934 t
ORIGIN      
        1 tcaacactca atcccctttt tgtacatatt cccccggggc gttaactccc cccactcggg
       61 tacgataggc ctctccacct cgatcagtca agagcacgca ggcgtcacct acgacccgct
      121 cacctgcgtt tgccggcgag tgatggtcag acgatgcagc cagttcaggt cgatacgcac
      181 ttctgtgccg atcgggcaaa acacgggcgc ggaggtattg ttactttcga cccgtgcctt
      241 cagcgcgccc cgctcgattt caatctccaa cacgcccaga tgttcatgca ggtcgatgac
      301 gcaggtggcg ctgcccgccc cgcgatcgcc aggaatcgtt cgtgccacga ccggctcttg
      361 caaggtgatg agatcatgcg gccggaacat cggccgatcg ttcatttcga ttatggtcat
      421 gatgacggcc ccggcgacaa tcaccgcgct tgccgcgagg atggcgttgg cacgctcggg
      481 atagtggatc ctgagccatc gaatcgccgt caggacgccg atggcaagca gcagtgcaat
      541 gaacgccagc accaggatga tgaacgcaaa gcgcaggttc atccggggcc gcccaggggg
      601 tgaacggcaa cgacgccatg cgctccattt gccgtcatca gcatcgtcga gctgatggag
      661 acggcaatct ctgcgccgtc cttatgcagg ccgacgcatg tcgggttgag cggtaacgac
      721 tctcccttcg tcgaagcgcc gcggtcagac gccaactcga atgtcccaaa attgaagagt
      781 gaaggaatga gccgctggag tgattgcccc gtcagatcgc ctgaggcata accgaataac
      841 tccgccaccc gtgtattcac ggccagaata ttcccgtcgc catccacaat cacgatgccg
      901 tcgccggtgg cttccatcac gatccgcacg agatcctgat tctcgcgaag cgcggcttca
      961 tgacgctttt ggtccgtgat gtcctgcgcg aagaccagca caccgatgac ctttcccgcc
     1021 gggtcgaggt agggtacttt gtctgtttgc acccagtgct tcttccctcc cgctcgatgc
     1081 ggctcgatga tgccgagttt cggtcgacct gacacgatga cttccaaatc gtcctgatga
     1141 taccgttcgg cgtcctccgg ataaaactca tcggtcgatc gcccttcgat gtccgccacg
     1201 gttttcccga tagactcggc cgctcgccgg ttggcccgca gaatgcgatt gtgggaatcc
     1261 ttgtaccaca ccatggctgg tatcagatcc aacagcgcct gttgctcgat ctgctgctgg
     1321 agcaggcgtg cttccgcttc tttccgtgcg gtgatgtccc ggacaatacc gcagtgcact
     1381 cgggtaccgc ttgaaatcca actactcagc gacatttcaa tgggaaattc ccgtccatcc
     1441 ttatgcaggc catgcatggt gacgacggtc cccttcaagc gcacatcgcc ggtggtgcgc
     1501 acccgttcga gagcccgctc gtgattgtcg cgatatcgtt cgggcatgat catggtcagg
     1561 gaccggtcca ggacttcctc cgcacgatac ccgaatagcc gctccgccgc actgttccat
     1621 gacaaaatac gcccttcccc atctgcaagc acaatggcgt ccggcgcggt ttgcacgact
     1681 tcgcgaaacc ggtcctcgct ggcagtgagc gcctgtttcg ccgaagcggc ttcgagcgac
     1741 aggtgcttga ccccgacggc ccgcttgatc agggccttca attcctcgcc gtcatacggt
     1801 ttggtgagat agccgaaggc cccctcggtg agcgacgcgt gtttcttggc tacctccacg
     1861 aacgccgtca gcatgatcac cggcagtgcc gggtcgatgc cctttaacag ggtcagcact
     1921 gaaaggccgt ccatatcggg caacatgaga tcgaggagca ctgccgagaa cgagtccgtc
     1981 ttcgccctgg tcagcgcttc cgcaccagtg acggcgacct gaacctgata tccggaatgc
     2041 tcgaggaggt ctttcaagac aagcgcaatg tcgggatcat cgtccacgat gagaatcgcg
     2101 ggagtgggca ccttgagggc actgggctgg gttctcagca tggcggtcaa cgaagcccaa
     2161 gggttctggt acgatccggt tcccgcactg aacggtattg tcctcttttt gacgacagga
     2221 atccagaccg ttagccggaa ttgcccctct caggtgcgtt gttgtcacac gcatgttctt
     2281 cgtctctccc gctcatggac ggaacagtcg tggtcaggca cccgcgcctc tattttttcc
     2341 gatagacatt gaggccgatt ttttcctccc gccccgtgat ggtgacattc ccttctgtgg
     2401 acgcgatgat ggtggtcttc cccgacgcgg acgggccgaa ctcctttgat agatccacct
     2461 tgatggtcaa ccaggttccc tcgacggaca tctcgacatt tttcataatg cgtccttatt
     2521 cgttaagcac gaacgtcact tttaacgcca cccgatactg cgtgatcttt ccatcctcga
     2581 tatccacttt ttgatcctcg atccacgcgc tcttgacatg ctgcaacgtc ttattggcgc
     2641 gggcaatacc ttgatgaatg gcatcgtcaa aactcttcgg ggatgcggcg atgatttccg
     2701 tcactcgggc aacagacatg atagcctccc tggttataga gttcgatgga tcctggtcac
     2761 ctcaggctag gccgacggca aagccctgtc aaggagccac ccccgtcttg aaccaggcag
     2821 catctggatt tctgccacag ctgcctccaa ttttgccggg aaagccttcc ccgctctccc
     2881 tgggtaaacc tctaggttga cttgcgttgt aacggggata taccgtgcac ccatccgcgg
     2941 tcgtagatca gtatgggaca ccatccatgc cgaccgagat cgagatacaa atcacccctc
     3001 tattttccac gcctctgctg gtattcactc ccgctgacca tacacgcatc aacgcccggc
     3061 tctcgaccat gattcttgaa cgggaagcgt ccgtcccggc ccatcgagat aacgaagtgg
     3121 tcggctggtc atcgcctcac gatctgtcca tgctcgaatg ggccggcgaa ccgctacgcg
     3181 atctgtttgc tccggtgttg gaagtggcga aacaggtcac caccttatcc cagcgctgcg
     3241 gacagggtac tggtcgaccg gagtgggatg tcgtagaagt ctgggccaat gtgcagcgga
     3301 acggcggcac caatgcggcc cattcccacc ccggtagttt ttgggccggg gtgtactatg
     3361 tggatgtcgg ggaggtttct tccaacaaag acatgggagg agagttacaa atctacgacc
     3421 cacgcgggtg tctgccgcgt atgctagcgc cgtatcttca gtacagcatg actgaattgc
     3481 acgatgcagg gaccagcatc tcttatgctc ccacgtcggg acagtgtgta ctgtttcccg
     3541 gctggctctt tcatgccgtg aacacctacc atggaaaagc cccccgcatc agtgtggcat
     3601 tcaacctcga tccggttctc gcgcccggca tgatgaacgg cacacagaaa cgtcctatca
     3661 ccgaatcccg ccgataatgc acccgttgat gatcgttccg gctacggcaa tgtgctcgcc
     3721 tgaatgaaca gcgcctgttc caatgtccgg aagaattgct ggtagggttc gggcgattca
     3781 atcgccttca gataatactg ggcactggcc cgcacgacca actgggcctc tccacgtggt
     3841 cgcacggtga cggtgagacg aaccagattg ctccggtggt aaaacggtcc ccaactacgg
     3901 aagtgtggag tgagaatcag cagcccgtcg tctcgataga gatggtagga agggtccctt
     3961 aatcgggtcg gttcgtaatc atcgaacttc tttgccgaaa cgatgcccag agcctcatcc
     4021 agcacttcga cggcaaaccc cagatcctga caggtagaga ccacggcctg aatcgtcttg
     4081 acccggtccg ccccgtcgaa tacccggctt tgcgccgccc gtaccttgac ctggctcgcc
     4141 tcggagagcc agatctggtc acgcgattcc cattgatttt catgtcgcca ctcatagggc
     4201 gaacagcccc ccgcgagcac cgctaggagc gcgaggctcc ccacgatcgg ccgaccgcca
     4261 gaccggcgaa atgagaacag cgttgagcga cgaggaccgg tcacgcgcta gtccttcacc
     4321 agaaacaatg accgctccac cgcggcaaaa aagttctgat aaactttcgg gtcctcgatc
     4381 ggcttactgt tatagatcgc attggcccgg atcatggtct tgccggcttc ttggggcctc
     4441 accgtcaccg tcactcccag gacatcataa taattgggtt cggcaaaacg cgccgcggtt
     4501 accagaccga gcggtgcgtt ggcacgttcg ataataaatc ccaaatcttg caaagccgcg
     4561 atgaccccgc gcatggccac ggcagaatca gtcacctcga acgtgcggct ctgcgcactc
     4621 cggattttca tctgcgcatc ggtcggcgcg aataattctt gcctcggctc cggagccaca
     4681 cagccgtgca gcgccagggc agctgccacg attcccaccg ctccgatgaa ttgtgtccga
     4741 ttcatgtacc ctcgccgtta aatcgaatgg ggttccaaga agacggcctt ggaaagcttc
     4801 gcaaagaact gctgataaat ttccggatcg cggatcattt ccatcttctg ctccccgggt
     4861 ggaatatagt tgcggtccgc ttggccatcc cccttccaga tcacccgata gaagatgatc
     4921 cggacttcct gcctcgcatc actgtggttt gccggacgga tgacaatcgt cgccgcaatt
     4981 ttttgatgta aatcgacggg gatcaccagg tgccccagcg acaccagcca gatgaggaag
     5041 cggttgcgat actgaccgta ctcccgggcg cttcgctctt tcgccgcgcg cagaagccca
     5101 acctctcgca cgctctcttc tatctgaaag ccgagatctt gcagcgaggc ggccgaggcc
     5161 gataacaatt cattatccgc cccggtctcg aatagccgcg tctgcatcgc ccgattggcg
     5221 ctgctctccg gcgagagttc aaaaaactgc gccggctggg tctgctccgc gcagccgcca
     5281 aggaacagcc cgcaggcaag caccagaatc atggcagccc atccgcgtcc gctgccagcg
     5341 tcaggccgct tagaattgag tcgagttata ggcaaagtct ctgacctttt tctcttcgtc
     5401 atacttgatg atgatggtca gcgtgcgctg gcgctgggag ctggagtggt cgctggagtt
     5461 ggcgcccaga atgatcaatc cggcatagga ggagctcgaa gtatcgacac gatccgtcga
     5521 caccttgtca tagatccaca cctcgcgccg cttatcatcg gtggtcacga tgttcggcga
     5581 gcccaggagt tccgccacct gcgccgcgga catgccgact ttcacttccc cctgcacacg
     5641 ccccacggtc aaccggtctt ccttgatttc ggcctgcttc ccgccgcaac cagtcaaacc
     5701 gatcacaaca cagatacctg ccaaggccaa tccccattgc atgtgtctca tggtctgcgt
     5761 ctccattttt cacaccatcc tctacgttat cgccaggggc acatcttagc ttctgtccat
     5821 cccggacgca aagcgtgatt cgccaggagc gtccggccga cgactgcctc cattgtcaga
     5881 tggctggaca attgatcagc agcagctaca atggtcgtag ggagggcggt caatgcctat
     5941 gccgatgcca gatgccatgc ggacggaaga ggtccaggcc gcgtgcgatg cacttgtcgc
     6001 gcgggtaaga accgccgtgc tgttgaccct caaacagggc caccccttcg gctctcatgt
     6061 cccctatctg atgggagacg attggtcccg ggtttatctt catctgagcg gtctcgcctc
     6121 gcacacccaa catatgctga aagacccacg cattagtctc tttattgccg agccggacgg
     6181 agccgggaaa aacccgtcag cgttgcaacg cctcaatctc caaggtagcg gcgccattct
     6241 ctcgccgtcc caccagtcgt atgacaaggt gaaggagcgg tacctgggac aatttccaca
     6301 atcccgaatg ctgtttggct tcggcgattt tgctatgtgg gaactgcaca tgagtgacgc
     6361 ccacctggtg ctcggattcg gccatgccta ccaggcccac tctgacaatc cgcgggagtg
     6421 gcagcaccag aagcctgaaa cgaaaccctg agccatcgca tcccggccgt tttttctctg
     6481 atctagccct acctcgaggc tgacactcca gcgggcgctc tcccctccaa agcactaccc
     6541 acttggccga aggaccgatg gtgcgctccg cgtcctgcac agtactctgc gcagagtcac
     6601 gtgagctcac cggagttgtc ccctacatgg tatttgccgc tcccatcatc ctgagttaca
     6661 tgctgttgct ggtcgggctt gccgtggccg ccccgggcgt cggttggggc ggttctctca
     6721 tcgaatttgt acccgaagcc tggcgtgatg gagcacacgt gccggcctac ggggtatttg
     6781 cctggttagt catgtgggga ctgcttcgcc gcgggtggcc cgtcactcat gcgacttcca
     6841 tcggaatcct ggcaacgggc gtattcggcc tctggacgga agtggcgcag ggcacagctc
     6901 ctggacgaga agtgtccctc catgatgtgt ggagtgacct gctgggaagc acgatcgccg
     6961 ccgcgttcat gctgtggcgt gaaaaggcga tcaggatgtc gaaaggtttc atggtggggc
     7021 acaagaggag ccatcaccac ctgatgaaag gaacaaggtc gagatgaacg cttcgtatcg
     7081 ccaacgtggc accagacgca tacccgtgca atacccgatc tattattcga atggcgcgtt
     7141 tcaaaccatc ggcgtgaccg aagacttcaa catcgaaggg gggcgtgttc gcgggagaga
     7201 agccgtgacg gtcggaatgg agctggtggt cattgtgatt caggcgatcc ctgaaacccc
     7261 gatgctgatc cgtcgcgcaa ctgtgcgttg gtcgaaaggc catgacttcg gcatcgccct
     7321 gtccggcgtc ccgcctcaat cggaagccga acttaaagcc atggccaaag tgatgttgcc
     7381 gggcctctgg tcctgcctga actgaacgct atttccggtg cgacggatcc aaaggatgcc
     7441 gcggaccggc aaaaaacatc tccatcaagc cgaagaacat catgaggagc gcgccaagtc
     7501 cgatcgccac agcccagttc tcccacgaca taatggtctc ctctcacacc ctgggaatcg
     7561 cccatcgatt cccagggtga tcagcctacc ttcccgatcg atgcaactgc agtaaggtct
     7621 cgagcttcca gagcgggatg ctgtccacct cgccctttcg ggtgctgaac gccaatccac
     7681 ccacgaacag ggcgatcagc ccgacacaag ccagaatcag ggagccgcga gaaacgtcgg
     7741 cgctcgcagc ccgatgatga gcgatcatcg tccggtcagc cgacatgttt tccaggatcg
     7801 ccaatcggtc catcaacgca tcggtccgca ccgccgcaac cgtcgcgctc gccaactgga
     7861 tctgccggtc ggccttcgct tccgcatagt gttcctgagc ctgagtgacc tgaacaacgg
     7921 cccgtccgac tcgttcctga gccgtggcgg tcatccggtc ttcctcttga atggcctcga
     7981 tgattgactg ccccagcgcc ggctgcctcg tggcctcgaa ttggtcgtgc atccgctgcc
     8041 cggtcaattc cgccgtcctg atcagattgg tattgaattc gccgccatac tggtcagggg
     8101 aaagcatccc actcgagacg ccacgccgcg tctggttgac gaccgacttg cccatgacat
     8161 attgcatgcg cgcgcggtga tcagtgtcct gtccgaaggc gcgcaattcc gccagcacga
     8221 gcggacttaa caacgtggtg gagattcgct cagattcgct gacagcgcgg ttcaattcca
     8281 tcgcagaggt cgacaaggct gcgccggcat cacgctccag caacagatct tgcacgatcg
     8341 cttgccctaa caccggttga agatactgca taccggtgga tggttcattg ctggcgatga
     8401 cactgctcat ggtgggagtg atcggagcct gccaaaatcc atcggcagcc gtcacgagca
     8461 gataccctcc gaatagtgtg gccaccaacc cgatcgctgc cattagatcc acgaggtcat
     8521 atttgcgtcc catgacaccc tccttcctgc ctcaggtaat ccggcgcggg gcaaacaggc
     8581 aggatgccgg cacgccgtgg gtagaacggc acgggatccc tgtcgtctgt gcataaagag
     8641 gcctcctttg aaaaaggtga tacctgcgct ccctgcatgt atgccttcgg catggcacag
     8701 acgaagacgc tcgtacgtac ctgccaggag atattaacgc gcgggggaac ctgagggagg
     8761 aaagggacaa gactggcttg gtcattgccg cggtcatgta tgccacggga gaccatgtga
     8821 ccacctgccg aaagggtggt actgcctaag atgcaatcag tctacgctcc aagaaaacat
     8881 cggtcaagac gggaaaaagc gagcaccggc gcggcctgcc agccatcgag gaatgcctgc
     8941 gctcttctta cgtgtcgttc ccatcggacc ttcgccgtct ggtaggatgt ctacggtata
     9001 tgtgcctggt ctccttgtga gaggcgttgc atggcgatca tcacgacaat actgttgttc
     9061 gtcatgtggg cattgatctc gccacgctgc gacgccgatg cgcagtccct tccgtttcga
     9121 gaatcacatg gcaccaccgg cacgctctct ccattgggcg acggcagcgc catctattct
     9181 gatccacacg gcaatacgaa tccgacgcag ctcggatccg gcgtgccctc tcacagtttt
     9241 agcggaccgc atggagccac gccggggacg ctgacgcctt tcggtacgcc caccccgccg
     9301 aatctgctca caccggcccc tctgctcccg ctacagccca aaggcatggc tatcccgcag
     9361 ccgcaatcgc ctgcctcccc ttctcctcct ggtcgcttcg ctccaccagg cggacgcccc
     9421 gggcggtaat ccacggagtg gtgaactgca gatcaaggcg atacaaacgc attcgcccaa
     9481 acaggactgt tgaatctact caaggctttc gttaagttac ggctaatgac catctcccgc
     9541 gcacgcctca ttctgcttgc ttgcgcgagc ggttttctct accccctgtc atttccggca
     9601 ttcgacctcg gcatcgtggc ctggtttgcc ctcgtccctc tgcacatcgc cgtggagtat
     9661 gcctcgccac ggcgagcctt ccgggtggga tggctcgccg gtaccctggc ctttaccggc
     9721 gccatgtttt gggtgatcac cgccatgaat gtgtatggca aggtgccgct gccgattgcc
     9781 gtcctggtga tgttattgct cacggtgtat ctcggcctct atctggctgt ctatgccggt
     9841 gcgctgtcct ggatcagaat agccttccct acgctcggat tcctgccggc gcccttcctg
     9901 tgggtgacgc tcgaattgat ccggacctat tttctctccg ggttaccctg ggcactgttc
     9961 ggctattccc agtaccaatg gctcgccatc attcaattcg ccgaccattt tggagtgtat
    10021 ggggtgtcgt tcctgctcgt gctggtcaat gccgcgctgg cggaaacact catttggagt
    10081 ttcaaagcct atcggggatt tcaaatccgt tccgttccct ggccctcact ggttgcagcc
    10141 gcctcaggat tcggcgtggc actcttttac ggcacgagcg tcctgtccgc cgcgcaacat
    10201 gccacacccc gcatgctgtc cgtcggtctg gttcagccga acgtcgaaca ggcacagaag
    10261 tgggatgtgg cgttccgcca ggaaaccatg tcccgctatg atcggttgac caccgggttg
    10321 ggtgagaatc tggatctcat catttggccg gaagccgcca cgccctttgt cttcgagatg
    10381 gagccgcaat accgggccat cgtcaacgat atggcacggc gcgctgacgc cccgctgtta
    10441 ttcggcagtc cggcactacg gcgttatccg gatggcaagc cgtttttgct gaatagcgcg
    10501 tacctgctgg cgaccgatgg ccaaatcctc gggcgatacg ataaacaaca tctcgtgccc
    10561 ttcggggaat acattccgtt tcacaactct ctgctgttct ttctcgacaa gctggtggaa
    10621 ggcatcggcg attttgaagc cggtccgggc tcgacgctgt tgatgttcaa gccgcatgaa
    10681 aaaactccac ccatgaccgc tgcggccagc aacgcggatt tccatctaaa attcggcgtg
    10741 gtcatttgtt atgaggtgat cttccccaat ctcgtgcggg agtttgccgc caacggggca
    10801 gacttcatgg tgaccgtcac gaatgacgcc tggttcggcc cctcgtcagc tccctatcaa
    10861 catttcggca tggtggtctt tcgcgccgtg gagaatcatg tggccttcgc gcgggccgcc
    10921 aataccggca tttccggctt catcgacccg tatggccggg tgctgcaaca gtctcccatc
    10981 ttcacgcaag aagcgttgcg tggaaccatt cccgtgagtc accaacagac cttttactcc
    11041 caatacggtg atctctttgc ctatgcctgt gctatactca tcgcgctttt gtgcctgacc
    11101 ggctacttct cgcctaatgc cgaaccagcc ggcggcgaac ctgccgggcg accagcctga
    11161 gagagaaagg accgtgccat gttggatgat gtccggaccc gcgtgcgaac ccttggtgaa
    11221 cagttagcgc agctacgggg gcatctttga cctggctcgg atgaccgctg agctcaacga
    11281 catcgacgtc aaaacctccc agccggattt ctggaaagat acgcaggctg ccgccaaggt
    11341 gaatcgtcgc aaggccgccc tggatcggga actttcacgc tggcgcgaca ccgaacgccg
    11401 tcagagcgat atcgaggcgc tcctcgagtt ggccttggag tctggcgacg ccgcattgga
    11461 gcaggaactg acggccgaac tcgagccctt cgaaaaagcc gtcgcccagt tccgcatcga
    11521 gttgctgctc tccggcgagt tggatgccaa caacgcgatc ttcgccatcc atcccggggc
    11581 cggaggcacc gagtcgcaag actgggcgca aatgcttctg cggatgtacg ttcgatgggc
    11641 cgagcagaag ggattcaaga tcgagacatt ggacttacag gcaggggaag aggccggcat
    11701 caagagcgcg accttgtcgg tcaacggccc ctatgcctat ggctatctga aggctgaggc
    11761 cggcgttcac cgcctggtga gaatttcgcc gttcgacgcg aacaaacgcc gccacacctc
    11821 tttcgcctcg gtgttcgtct atccggagct cgacgatgac gtagaggtgg tcattgagga
    11881 taaggacctg cgagtcgaca cgttccgggc cggcggcgcc ggcggacaga atgtgaacaa
    11941 ggtagagacg gccattcgca tcacacacat tccaaccggt atcgtcgtgc aatgtcaaaa
    12001 cgaacggtct cagctccaaa accgtaacgg agccatgaaa atattgcggg cccgtctctt
    12061 tgaagtggaa caacgcaaga aggaagcgga gttcaacgcg atcgtcggcg aaaaaaaaga
    12121 tatcgcctgg ggcagccaga ttcgctccta cgtcttccag ccgtaccaga tggtcaaaga
    12181 ccatcgcacc ggccacgaag tcggccaagt gtctgcggta atggacggcg acctggacgg
    12241 cttcatcgaa gcctatctgc agagaaaaat gaccggcggc aagactcccc agccggtggc
    12301 catgacagat gacgacgtat aaactgtgac ccgcctctcg tgacgcctgg tgcccgcgtc
    12361 acgtgtgaag agcgacgaac gacgagagac ccatccatgg atgaattaaa cgatcaacgg
    12421 caacaacgca tcaagaaact cgaccagctc cgacagctcg gggtcgcgcc ctatggcacg
    12481 cgcttcgaga tcaaggaccg cgccgggcag ctcgtgcagc tgcatgggga aaagagcaag
    12541 gaggtgctcg agcaagaaca ggtcggctgc accatcgccg gccgtatcgt cgggttgcgc
    12601 cggttcggca aagccgcgtt cgccgtgttg caagacgggt cggaccgtct ccaggtctat
    12661 ttgaagaaag acacgctggg tgaccaggcc caccagattt ccgagggact ggatctcggc
    12721 gactggatcg gcgtgaccgg tatcctcttt cgcaccaaga ccaacgagtt caccgtcgag
    12781 gcgaagacgc tcacgtttct cagcaaggct ctccgcccgt taccggaaaa atggcatggg
    12841 ctgacggatg tcgaaaccag gtatcggcaa cggtatgtcg acttgatcgc gaacccgcaa
    12901 gtgcatcaga ttttcgcact ccgcagtcgc atcatctctg ggatccgcgc ctttctcatc
    12961 gagcgtggct tcctggaagt ggaaacgccg atgatgcatc cgattcccgg aggcgcggcg
    13021 gccaaaccct ttgtcaccca tcacaatgcc ctggatgctg aactgtatct ccgcatcgcg
    13081 ccggagctgt acctcaaacg gctgatcgtc ggcggatttc cccgcgtgtt cgaaatcaat
    13141 cgcaacttcc ggaatgaagg catttcgacg attcacaatc cagaatttac gatgctggag
    13201 ttttacgtcg cctatgccga ctaccaggac ctcatcatcc tgaccgaaca actcttcagc
    13261 cgattggcgc aggacattct ggggacgatg accatcgact atcagggcgc gcagatcaat
    13321 ctgggatctc cctggcgccg ctggtcctac catcaggcga tcctggaagt gaacagtctg
    13381 cccccctcgg tcctcacgaa tcgcgacgaa gccctcgccg ccgccaaacg gctcggtgtc
    13441 gaggtgtccc cgaaggaatc gctcgtcaac atcctcaacg agatctttga agagaccgtc
    13501 gaaccgaaac tcgaacaacc aacctttatc actgactatc cgattgaaat ttcccccctc
    13561 gcacgacgca aggattccga tccttcgttg accgaccggt tcgagctgta catcgccggg
    13621 cgcgagatcg ccaatgcctt ttccgagttg aacgatccgc tcgatcagcg cgagcgcttc
    13681 gaagctcagg ccgcccaaca tgcggccggc gacgaggaag cccatcgggt ggacgaagac
    13741 ttcctgcgcg cgctcgaata cggcatgccg ccgactgccg gccaaggcat cggcatcgac
    13801 cgtctcgtca tgctcttcac caatcaggct tctatccggg atgtcgtgct cttcccgcaa
    13861 ctcaggcctg aaaagtagac atggcgcttc cctacgaaat cttcatcggc ctgcgctacc
    13921 tgcgcgccaa gcggcgtaac cgcactatct ctttcaatac gattgtgtcc atcgtcggga
    13981 ttacgctcgg cgtcgcggcc ttgatcggca ccgtcgggat catgacgggg ttcaaggagg
    14041 atgtgcaggc gaagattctc ggcaccacgg cccatattct cgtgaacgac cgtatgaagg
    14101 aatcgatggt cgattacgag gaacaggtga agaaggtcgc aggtgttccg gaggtgctgg
    14161 cggccacgcc gtttattttc cggcaggtcc tgctcacgtc tccatccggc gtccaaggca
    14221 tcatcctgcg cggaatcgat ccggcgcgtg aaggcactgt gaccgagctc gcgcacaacc
    14281 tggtgaatgg caacctcgac gatctctcac attccaccaa agttgtcatt ccggaaaagg
    14341 aacgcgagcc gaacggaccg gataccgcca tgcggccggg catcatcctc gggaaagagc
    14401 tgtcgatgcg actcggcgcc ttcccgggag acacgcttaa tgtggtctct ccggttggac
    14461 ccgtgaccgg cgccagcatg acgccgaaaa ttcggcaatt cgtcgtggtc ggcatcttcc
    14521 aatccggcat gtacgaatac gattcgtcgc tcgcgtatat cgaactggga gaggcacaga
    14581 aattttttaa catgggcgcc acagtgacag gcatcgaggt caaagtcgcc gatgtgtttc
    14641 gcgccgccga cgtggcccgg tcgatcgagc agcagcttgg cttcgccttc tgggccaggg
    14701 actggatgca aatgaaccgc aacctgttct cggcgttgaa actggaaaag acgatgatgt
    14761 ttttgctgtt ggtgttaatc acgattgtgg cgtcattcaa catcgtcagc accctgacga
    14821 tgatcgtgac ggaaaaacag cgcgagattg cgatcctcaa ggcgatgggt gcgacgcgta
    14881 aaggcatcat gcggattttc atgctcaacg gcctgatcat cggctgttca ggcgcggcca
    14941 tcggcgtccc gctggggtat gcctttctct ggttgattca gaccttttgg acctttgatc
    15001 ccacggttta ttacatttcc cgtattccgg tccatgtcct gggatcggat gtgttgttgg
    15061 tggcaggctc ggccatcctg attagttttg ccgccaccct gtatccatcg ctccaggcgg
    15121 caaaactcga cccggccgcc gcgcttcgct atgagtgacg cgtatggcat gccaagacat
    15181 cgaccagcgt gaagcagtga tcatccacga ttccatagcg cagtgaccgc atgattgaca
    15241 ttgtcgacct ctacaaaacg tttcccatgg gcggccgcga gttagtggtg ttgaataaca
    15301 tcaacttgcg gattcagcgc ggcgagctca tcgccatcat gggcgcgtca ggagccggca
    15361 agagcaccct ccttcaaatc ctcggcacgc tggatcgccc caccaaaggc accgtgtcgt
    15421 tcgacggaca gaatctcttt cagctcaccg agcaacagca ggcggagttc cgcaataaac
    15481 gggtaggatt cgtctttcag tttcaccacc tgctgccgga attcacggcg ctcgaaaatg
    15541 cttgtttgcc ggcaatgatt cagaaacgcg agatggccga tgtcgtcggc gaagccacga
    15601 agctcctcgg cgaggtcggt ctggccgatc gcctgcacca caaaccgggc gaattatccg
    15661 gtggcgaaca gcagcgggtg tccgtcgcga gggccttgat gcagcaaccg gatctcgtat
    15721 tggccgatga accgacgggc aacctcgaca gccataccgg cgacgcgctc ttcgccctca
    15781 tgcgtcaatt gaatcgctcc cgaggtacaa cattcgtcat cgtcacgcac aacgagaaac
    15841 tctccgccca ggccgatcgc atcatctcca tgcaggacgg cacgattatt tcgtcgtaga
    15901 cgacacgtca ccgaccagct ccgggcggca atcatttctt gacggcttca gcatgttttc
    15961 gtagactgcg ccatcgatcc ttccgagccg gagttctact gaatctatga acagacaggt
    16021 catgtcctac gttcaataca cagccgtcct gcttgccgtc ttgctgccgc tgcaggcggc
    16081 ggcagtggaa tttgtgtttg tcggatcacg gcagatgggt atggggggag ccggagtagc
    16141 cacgacgacc gactccctcg ccacctattg gaatcccgcc ggcatggcga tgagcaagaa
    16201 attcgatatg cggattcaag gcggtggcca ggtggtggat cgcggtgacg tcttcgatac
    16261 gatcaaggac atcaaccata tcaatttgaa tgatacctcc gcccccaata tcgcccggct
    16321 gcaacagcaa atcgatcgga tgaacaaacc gggcacgaac ctgacggcag ccgcctccgg
    16381 cggattctac gtcaagggca acttcggcga acatgccttg ggattcaatg tctcggatgt
    16441 cgccacatcc ggcggctttc tgcggggccc ggtggggttt acgaataacg gcacgaatct
    16501 taccgtcaac gggcaattcg ccatgaacgg cctggaagtc cggcaggcgg cgctgtccta
    16561 tgcctatgcg ttcatggacc ggatgttttc catcggcgtt accggcaagg tcatccaagg
    16621 cgcggcctat accggcgcca ccaatattcg cggcgcgagc gacgacgtaa aggtcttcga
    16681 agacatcggg cgcgcaaaaa tctcgagtgc gctcggcatc gacgtgggcg ccatgttccg
    16741 cccttcgtcc tggttgcgga tgggcatcgt cgcaaaggat atcaacgcac cgacgttcga
    16801 tgccccgaac ggcgacaaat tcaagctatt gccacaggtc cggaccggca tcgccgtgaa
    16861 cccgtacaac tccctcacat tgaccgcgga tgtggacatt acaaagaaca acaccctgac
    16921 gcccggggtg tacagccagg tcctcagtct cggcgcagaa cagacgattc tctccgaact
    16981 gctgtcgttc cgactcggcg cgtttaaaaa tatgcaggat gcgaaaacac cctttatccc
    17041 gacggcaggc ttcggcatac gtatcttggc cttgcgcatc gatattgccg gcgggtatga
    17101 tttcagggac caggctgcac tggcctcggg atccatcgcc gtgactttct aaggtcgcgt
    17161 ctgcagtgaa tcaatctgct acacttattg tacgaaccat gactcgacac acattttcca
    17221 cgatcagcgt tgtgctctgt ctcgtgatga ccggactgct ggcaggctgc ggaggcttgc
    17281 aggagatgtg ggaaggaccg gctgcgcggg gtttttaccc gaaaacgatg gcgattctgc
    17341 ccatctccgg cacctacgac agcgcgcgtg aagacgtgga agaagtggtc gcgaaggtgc
    17401 tgatcaagag ccgccgggtc gaaaaagtcg tcccgcctga tcaggtcacc gacacgttcc
    17461 agacgaccaa agacgctttc gatgcactcg tgacctattt ttcgcgcatg gaaacaaccg
    17521 gccaatcgga caagaattcc gcgataaaga tcgggcatgc cctgggcgcg gattccctgt
    17581 tggtggtcaa ggtgaatgcg tgggaataca caagagagga aggcgataac atcgcacgcg
    17641 tcggcttgag tatgcgactg gtggacgccg tcaacggcgc catcgtctgg aaagcacgcc
    17701 accaggtgca ggaaagttat ctgttcatcc gcccggatat gaaggacctg gccaccaaac
    17761 tggcggccga catgatcaag tacatgccgc cggaaaaacg ctgaccctct gtgcgcccgt
    17821 cacgccctga cgcctgatcc ccttctcaga ttgtcgattg ccagcccgaa ctgattggag
    17881 agactcgcca gctccaacac gtcccagttg ctgaatggtt taccactgcg ccgattgacc
    17941 acttccaaca cccctactgc ccgcgcgccc atgcgaaccg gaacagacac gagcgaagcg
    18001 accgagagag cggcttgcca tccctgccct gcaaccagtc gcggatcctg acggccctga
    18061 gtgatgagca ctgcctgacc cgtgcgatgg actcctgcgg caacggtcga atgcaaggct
    18121 gtgagtcctg cggcgcgttc tccgccgaga gcagacaagc tcaatgtccc gtcgggggat
    18181 gcgagaaaca acgcaccggc ttcggcttca accagccgaa ccgccgcatc cagggcccgt
    18241 cgcaattttt cctcctctgc cagagaggaa ttcaagatat cgctcacctg ctccatgtgc
    18301 tgaatgcgaa ggatcttggc ggcatatttg accgtcaacc tggcgtggtc cagcaggacc
    18361 gccagccaag gttccaccgc cgtcagcagt gacaggagct tgcgcgaggg caatcgctgg
    18421 gacttccctg cccggaacat gaccacccca tccagccggt tgatccggtg taatcgaact
    18481 ccatgcagct cgccactgac aatttgagca ttctgctccg ctttcttcag tccctccagc
    18541 cacaactccg tcactgaggg atcgctgctg ccggcttctg ccagacaggc catctcacgg
    18601 gtttgctcgt cgcgtacgta gagcgcgaca gcctggacct tggtcagctg caggaataac
    18661 tgtacgaata ccgcagccac cccgattggc gaacgcccct caccttctgc actcgacagg
    18721 gtattgaaca aaccctgctg cagcgtacgc aggagccggt cttctccaat aggagctggc
    18781 ttgcggacgg tggtttttct ccgcgcggcg actgatgagt tcgtggatgt cgtcttacgg
    18841 gtggtacgac tgatcttccg gcgagctacg gccatggcat ctcctcatgt gaaccatcga
    18901 tcgttgcagg acagagcgta accgctctgc atgattgttg ctggaattga tagcgggtga
    18961 ggaagcgaga tggatgcgag tcaggcctga tgcggacgag tcgcctgcaa tcggcctgca
    19021 aatagagttc gaaactatcg aaggtgatga cgagcggatg ccgatgcgaa cccggttatc
    19081 cgggctgcat cgcagctttg agagagcgaa cgaaggaacc cacttcgtga acgagtcggc
    19141 catcctgccc atgttctcca acacgacgga caatcgcact cccgacgatg acgccatccg
    19201 cagtctcagc aacccgcgcg gcatcctccg gcgtggcgac cccgaatccc acggcaacgg
    19261 gtgtcttggt gtgctttcta atcttggcga cgttgtctcg cacgcccgcc atgtcgttca
    19321 gtttagcccc agtgatgcct gtgatggaaa cataatagac aaatccgccg gactccttcg
    19381 ccacgtaggc ccggcgtgac gccgtgctgg tcggtgccag gagaaaaatc aggtgcaagt
    19441 cagctgcagc tgccggctcg cggagaggcc cggcttcatc aggtggcata tccggaacaa
    19501 tcagaccgtc gactccggcc gcggcagcct tgctgcaaaa gtccgcctcg ccgaacgcat
    19561 gaatgttgtt gtaataggcc atcaggacga tagggatctg ggtcatgtcg cgaagacgcg
    19621 tcacggcttc gagaatcctg cggagcgagg taccgctttg caatccccgt tcggctgcct
    19681 gttgaatcac cggtccatcc gcgatgggat cggaaaaggg gacacccaat tcaatgatgt
    19741 cggcccctgc ccgctccagt tcgagcacca actgttccgt gtcctgcaat gaaggatcac
    19801 cggccatgat ataggtgatg agggctttct cccccttcgc tttcaaccgc ccgaatgtct
    19861 gatctaaacg attcatctcg atatacctct gcctctccat cgtcctaaaa gctaatcaca
    19921 ggaggttcaa aacgatcatc cagcgcggcc gcagcaagcg cggaggtgag gcgtactcct
    19981 gcagtacgtc gaacctctgg ccgccgcgag acgcagcggg cagcccgagt caacatcctg
    20041 ctacagggtc acgcctttga ttcgggccac ctgctggacg tccttatcgc cacgtcccga
    20101 cagattcacc acgatgatct gattcttttt catgcggggc gcacacttca ccacctcagc
    20161 gatggcgtgg gcgctttcca gtgccggcat gatcccctct tcccgcgcaa gcaagtcgaa
    20221 ggcggccaag gcctcatcgt ccgtcgccga tgtgtattct gcccggttgg tttccttgtg
    20281 ataactatgc tccggtccca ccgccgcata gtccaacccg gccgacacgg aatgggtaag
    20341 attgacttga ccgttctcgt cctgcagcag gtaggtcatc gttccctgga gtacacctgg
    20401 acgcccgccg gcgaaccggg ccgcgtgttt gccgctctcc acaccaagcc ctgcggcttc
    20461 caccccgacc atcctcacct tcttgtccgg cacaaaggcg tgaaacaacc ccatggcatt
    20521 gctaccgccc ccgacgcagg cgatcagaca atcagggagc cgtccttccg cggacagaat
    20581 ctgtttgcga gcctcgcgcc cgatcacggc ttgaaaatcg cggatcatca tcggataggg
    20641 atgcgcaccg aggacggagc cgagcacata atgggtggtc cgtacattgg tcgtccaatc
    20701 ccgcatggct tcgctgatgg catcttttaa ggtccggctg ccggcatcca cacccgtgac
    20761 ttttgaaccg agcaacctca tccggaacac gttcaacgcc tgccgctgca tgtcctccgt
    20821 gcccatgtag atttcgcatt cgagcccgaa catcgccgcc accgtcgccg tcgccactcc
    20881 atgctgaccc gcgccggtct cggcgatgag ccgcggcttc ttcattcggc gcgcgagcaa
    20941 cgcctggccg atggcgttgt tgatcttatg ggcaccggta tgacagagat cttcccgctt
    21001 caaataaatc tttgcgccgc ccagccgctt cgtcaggcgc tgagcaaaat acagcggcgt
    21061 cgggcgtccg acatattccg tgagataatg tttcagttcg gcttgaaagc tcttgtcctt
    21121 gcgggtgcgc tgatacgccg cttccagctc cagcaacgcc ggcatcagag tttcaggcac
    21181 atatcgcccg ccgtagggtc cgaaccgccc atgtcgatct ggtatcgcca tgtgtcttcc
    21241 ttcagctaat cgtaaaagtg aaagtataac aggatgatga aaacgtccgc cagcctcggg
    21301 cccacaacga agggaactat tatcgcggga gccgcttcac ggcttccaga aacgcccgga
    21361 cttttgcatg atcttttttt ccgggcttcg cttccacgcc gctgctgacg tcgacgccat
    21421 atggatgaac ctgctcgatg gcttgaataa cgttgtcggg agtcagcccg ccggcgagga
    21481 gaatgcgcgc aaccgatgca gcttcggcgg ccaatgacca atcggccacc tgccccgtgc
    21541 cgccataggc gtcgggagaa aaggcatcca ccaaaaaacc gcgcacgccg gcgcggccct
    21601 gaaattctgc caacgccaga aacgatcgac gatctctgag gcggatggct ttgagaaccg
    21661 gccgtcccaa ggattcgcag taggccgcgg tctcgtcgcc gtggagttgc gccagggcca
    21721 gaccacagct atccatgatg tcccgcacga ccttggcctc ttcattcacg aagacgccga
    21781 tcggcaggac gaagggaggc aattccttga cgatcgcctt cacggcctcc gtttccgcac
    21841 agcggggact cttcttgtgg aagacgaacc cgacggcgtc agcgccggcc tcgacagcca
    21901 ccgctgcatc ctccgcgttc gtcaacccgc agatttttac cttcacggtc atgcgtcaac
    21961 aactcgtctg acttttttgc atcccgtaaa atccgcgata ggaccactgg caggcttgcc
    22021 ccttgggatt gaaagtcagg ccgatcaaat cctgtcgccc atcctgctga gggatcatcc
    22081 actcgcacac ttcgcccccg tctttaaacg aatgacagcg cgtggggatg cccatctctt
    22141 tgatccggtc gtctttcgtg ctgcccatcc aggaatccca cttgggagag tattcatgaa
    22201 tttcctgatt tacgcagccg gccaccgtca atcccatcgc catcagaccg aacaacagca
    22261 gcgtcctcat gattgctcct tggttggcgg atcacccagc agttcgcgaa tcttcgcccc
    22321 ggtatcctgc gcgcgaatca acgattcgcc gatcagcatg gcgtggacgc cggcttcgac
    22381 caaccgctca acatccttgc gtgtgtggat tccgctttcg ctgacgatca tcttgtcggc
    22441 gggaatccgt ttcgccaggc gaagggtcac gccgaggtct gtcgagaagg tcttgagatc
    22501 gcggttattg atcccgatga gccgtacgtc cggcaaccat tcgagcaccg tgtcgagttc
    22561 gcgctcatga tgcgtctcga cgagcacatc aagagacagc tctttggcca atgccgaaaa
    22621 gtcgatcaac tggcgttttt ccagaccggc cacaatcaac aggactgcat cggcgccata
    22681 ggcccgagcc tcgtaaaact gaatgtccgc caccatgaat tctttgttca gcgcgggaag
    22741 gcccactgtg cgcttcacat cggccaaata cgccaggtcg ccttggaaaa aatccttgtc
    22801 cgtcagcacg gacaccgccg acgcgccatg ctcgcggtac gcttcggcaa tcttgaccgg
    22861 ctcgaagcgt tgctcgaact cgggccgcaa aaggccgaga ctcggcgacg ctttcttcac
    22921 ttccgcaatg agcgccggcc tgtcggcggt gcgtcgcgcg tcgagcgtga ccgcaaaccc
    22981 cagcgtcgga cctgcgtcgc ggattctggt tttcaggtca gccagatacc cgcggctgct
    23041 cttatgacga agttccgctt tcttgtgttc gagaattcgg tcgagaatca cggtgtcacg
    23101 aggccttctt ggtaaatgcg atcagttgtt ccaacttttc catggccgcc cctgcgtcga
    23161 tcgtccgctg agccagctcg aacccttctt gtaacgtctt cgccttgcgc cccgcgacga
    23221 gtgcggacgc cgcgttgagg cagacgatat cgcgcttcgg gcctttgcgg cccctgaaaa
    23281 tgtcccgcgt gatggcggcg ttgtcttcgg cgtttccccc gacgagatcc ttcggcctga
    23341 cgcgcgcgag tccgaactct gcggggtcga tcgtgtaact cgacacgacg cccgccttgc
    23401 cctctgaaat tctggtgcga tccgtcacgg taatttcgtc caggccgtcc atcccgtgca
    23461 ccacaaaaca atgttgcgcg ccgagatgca ccagcacctt ggccagcaac tcggtcaggc
    23521 cggcatcgaa gaccccgacg acctggagac gggctccagc cggattggtg agcgggccga
    23581 gaatattcag caacgtgcga atgcccagct cctgccgggg ctgggcacaa tgtttcatgg
    23641 cgctgtggta caagggtgca aacaggaaac caatgcccac ctcgttgacg caatcggcca
    23701 cctgttcagg cggcagctga atggcgacgc ccagggcggc cagcacatcg gcgcttccgg
    23761 acttcgacga cacggagcga ttgccatgct tggcaaccgt gattcccgct cccgcgacca
    23821 cgaaggccgc cgtcgtcgat atgttgaagg tgtgggcccg atcgcctccc gtgccacacg
    23881 tatctagcac gagtgaatcc ttgacccgaa tgtgcgtcgc cctggcgcgc atggccaaga
    23941 cagagccggc gatctcttcc accgtctcgc ctttcatgcg caggcccatg aggtacgcgg
    24001 caatctgcgc gcaggtggcg gatccgtcca tgatctcgcc catcaccgtc tcagcttcct
    24061 gctccgtcag gtcagcccgc tcggcgagtt tgttgatggc atctttgatc atgggtataa
    24121 cacgatggga ggcggcgcac cggccctcac gctataactt gaggaagttg cgcagcagct
    24181 ccttaccggc ggtggtgaga atcgattcgg gatggaattg caccccttcg atgcccagcg
    24241 tcttgtgccg catgcccatg atttcgccct cggcggtttc cgcgctgatc tcgaaacaat
    24301 caggcagatt cttccggttc acgatgagcg aatggtaccg cgtggcttcg aagggattcg
    24361 gcagaccacg aaacaaggtc cgcccgtcgt ggcgcaccat cgaggtcttc ccatgcatga
    24421 gacgctcggc acgaatcacc tccccgccga acgcgacggc aagggattga tggcccaggc
    24481 acacgcccag caatggcaac ttgccgccga agtgcttgat ggcctcaacc gacacgccgg
    24541 cttcactggg ggtgcagggg cccggcgaaa tgaccagccg gctcggcttc aaggattcca
    24601 tctccgggat ggaaatctta tcgttgcggt aaacgacgac gtcttcaccc aactcgccga
    24661 aatactgcac gaggttgtag gtaaaggagt cgtaattatc gatcattaag agcatggtgc
    24721 ctgcttgtcg ttccgcggtt tcacgcgtgc gtgttcacgc tgttattcca gtccctgctc
    24781 ggcgagttcg atggctttca tcatcgcccg ggccttatta cacgtctctt catactcatg
    24841 ttcaggaacc gaatcggcga caatccccgc gccggcttga atgtacgcct gccggccctt
    24901 gatcacgacc gtccggatat tgatgcacat gtccatattg ccggagaatc cgaaatagcc
    24961 gacggctccg gcgtaaggtc cccgcctcgt cggttccaat tcttcgatga tctgcatcgc
    25021 gcgaatcttc ggcgcgccgg acacggtgcc ggccgggaaa caggcacgcg tcacgtcgta
    25081 gacggatttg cccttttcca attgtccggt tacctgcgac acgatgtgca tgacgtgaga
    25141 ataccgttcg acctgcatca aggaatccac cttcacggat ccgcgagccg ccacccggcc
    25201 cacgtcgttc cggccgagat caaccagcat cacatgttcc gcccgttcct tttcatccgc
    25261 gagcagagac cgagccaggt cctgatcttc atcgggagag ttgccgcgac gacgcgtgcc
    25321 cgctataggg cgcaaggaga tctgcccgtc ttcgcaacga accagcgttt ccggcgacga
    25381 tcccaccaat tcgactccgg ccacgcgcaa ataatacata tacggcgaag gattgacgac
    25441 gcgcagcgcg cgatagagct gaaagggcgt cgtatggatc tgcgtctccc accgttgtga
    25501 caggacggcc tggacgatat ccccggcgcg gatatattcc ttggtccgcg tgaccatctt
    25561 ctcaaaatcc gcacgattca tattgggcgt gaacgtgatg ggcttccggc ggcgcttcac
    25621 ccggggctgc cgcaccggac gcttgagacg ggcgatcatc ttgtcgatcc gcgcggtcgc
    25681 atgtcgatag gcctcgcgga cggcgcgatc ggtcgtcgat tcgagatagg cgttcgccac
    25741 cactttgatc ttctgcgaca cgttgtcgaa aatcagcaag gtgtcggtca gcagaaaggc
    25801 gacctccggc attcccaggc tgtccttgcg gagggacggc agctcctcaa aggtccgcac
    25861 cacgtcataa ctcaaatacc cgactgcgcc gccgacaaat cggggcagtc cgggcaccgt
    25921 caccggccgg tactcctcca tcaattcacg cacgcgctcc agcgggttgc cgcggctctg
    25981 aatcttcagg cgtttcttcc cgcgagtcag aatcaggtcg cccttctctt catggatgac
    26041 cgctgaagag ccgcttccca aaaaggaata gcgcgcccaa ttttctccgc cggccacact
    26101 ctccagcaga taggcggtcg gcccctggtc gattttggca aaggccgaca ccggcgtctc
    26161 gtagtccgcc agaatctccc ggtacaacgg aatcaggttc ccttccgttg ccaggcctcg
    26221 aaattcgtcc agggtcagtg aatactgctg cttggtcata gagatgatcc ggctgatcgg
    26281 ttacggctgg ctgacgatca gcttttggcc caccccgacg gtgttatctt tcaagttgtt
    26341 ccacttgcgc aattgatccg gggcgacatg atatttctgc ccgatgcggt acaaggtctc
    26401 acccggtcgc accgtgtggg tcgtgccggt cgccgctgca gccgtcgctc cggcgccatc
    26461 catcagatca ctgtgagtga gcgcatcctt cgccgcgccc gtggccggct ccaggctcaa
    26521 gtccgacggc acctccgtct tgagcgcctc atccacagcc gccgcaaagt ccttcttcgg
    26581 cgccgctttt ttggccttcg gaatggtcgt caggcgtttc atatcctcgt gcgcccgctt
    26641 ctccaagagg ctggcttcct tcatcgcctg cgcctcttcc tggatctggg ccgcttgctg
    26701 acgaaccgcc tcgacctgcg cgcccagttc gcgattgcgg gcttcgtact cgctgagttc
    26761 ccgcttgccg cgcttcactt ccgcatccag ttccgcgctg cgtttttctt cctgcgcgag
    26821 aagccgctgg aagttgagac tgcgcgcctt ctccgcctca tatttctccg acatgacaca
    26881 tccgcccaaa cccaggctgc ccaatatcag cagaccgatg ggaactacgc tcctgcgtct
    26941 cagccgtccc tgttgttcac tcatcatgta gctccttcgt tcaccctctc gatgggaggc
    27001 atccatgcca gtcccaggga gaattctatg gcgcatgcgc tgccgattcg cagcctgaga
    27061 aagtgggtag aatacggtcc aaggcagcga aagtcaaagt gaaatgggca ggcacggtgt
    27121 ttgaccctct ccccgtgcct atgctacggt tcgaagagcg tatcgttctt cccattctag
    27181 cgaaccaccg gcacgaaaga ctatggcatc aggcgcacaa ccggcctcgt ctcgagtcat
    27241 ttccatcgaa ggcatcacgc tgcacctggc tcaacccatg agcatgggtc aggaatggat
    27301 cgggagtcgc gagatcctga aacagctgct ggcctgctgg ctggtcatcg acgaacgcga
    27361 cctgcccctc tcgcctcgga ttaccggtca gcccggaatc ggaaaaacca cgctggcgat
    27421 ggccggagcc cgtgagcgca agcaggatct gtatgtgttt cagtgcaccg cggatacacg
    27481 tccggaagat ctcctgatta cgccggttct ggcggaatcc ggcaccatca gctatcacgc
    27541 ctcgccgctc gtcacggcgg tcctcaccgg cagcatctgc gtgctggacg agggcaaccg
    27601 gatgaacgaa aagagctggg cctcgctggc tccgctactg gatcaccggc gttgtgtgga
    27661 atcgatcatc gcaggcattc tgatcaaggc ccatgcggac ttccgctgtt gcgtgacgat
    27721 gaacgaagac gcgtcgacct atgaggtgcc cgattatatt ctgtcccgcc tgcagccgac
    27781 cttagggatg gggtttcccg cgcgggaaga cgaactggca atcctgcggt atcacctccc
    27841 cttcgcgccg gcggagatgt tggcgctgac cgtggaattt ctccaggaag cgcaccaact
    27901 cagtctcgaa tactcggtcc gcgacggcat ccatctgttg caatacgcgt tgaaacggcg
    27961 cgcacaagat cccacagatc cgctctcggt cgacgcggcc tggcgggaat ccctcatcaa
    28021 ggtgctcggc gaggaagccc tcgacctgcc gatgcagtca cgccgtcgaa aacgcgccct
    28081 cggcgatcag gccctgccgc aaggactcgg agatttcttc tttggaagtg atgaccctct
    28141 acatcccggc cgataatggc agcaccatct tccaccgccg acgacgtctt ccgcctctca
    28201 ccccgcatcg aactgtttcc ggtccttcac ggcagcggtg atgtggcaca ggaagtgcgc
    28261 gaccggctga ccgatcgccg ctatgactgc ctggccgttc ccctgccgcc ctctttcgaa
    28321 cacccgcttg aagaagcggt cgtcgatctg ccgaccatca gcgtgattgt
//
LOCUS       DAOQEW010000023        28176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_381_length_28176_cov_8.541019, whole genome shotgun sequence.
ACCESSION   DAOQEW010000023 DAOQEW010000000
VERSION     DAOQEW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28176
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_28176_cov_8.541019"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..319
                     /gene="atpE"
                     /locus_tag="PLS35_04385"
     CDS             89..319
                     /gene="atpE"
                     /locus_tag="PLS35_04385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNI17925.1"
                     /translation="MDAAAAALVGMGLAAAGFAGAGVGIGYIFGKMIEAVARQPEAEG
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGLRK"
     gene            393..905
                     /gene="atpF"
                     /locus_tag="PLS35_04390"
     CDS             393..905
                     /gene="atpF"
                     /locus_tag="PLS35_04390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNI17926.1"
                     /translation="MPQFESHFFSSLIFWEILSFGILFFLLYKYAFPSLLGMLEEREK
                     KIKDSLDQAERHRSEAERKLKEYEAKLATAAKDAEALLAQAKERAARLMEENEQRMTA
                     DAERIKGDATREIETERRKAIQDIRSQTTDLAMMVAEKVVGRALTDGDHRRLADEALD
                     ALAKTYQSRN"
     gene            complement(902..1732)
                     /gene="larE"
                     /locus_tag="PLS35_04395"
     CDS             complement(902..1732)
                     /gene="larE"
                     /locus_tag="PLS35_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNI17927.1"
                     /translation="MVSSTPLIEKISRARHLLREMGSVIVAFSGGIDSTLVLKLAHDE
                     LGMQATGVTAVSPTLPASELALTQQLATEIGARHRVVETDQLEIPQFVRNDATRCYHC
                     KTDLYSLLGKLRSEDATACIVDGTNVDDLGDDRPGLKAAREHGVRSPLLEAGFTKADI
                     REAARELHLSNWDKPAAACLSSRVPRGITITRSTLSRVEQAEAALIQGGFRHCRVRDY
                     GDLARIELAVEELPKLLEAGCREQLVESLKQIGYRFVTLDLEGYRQGGVSLTPPASGT
                     "
     gene            complement(1780..3153)
                     /locus_tag="PLS35_04400"
     CDS             complement(1780..3153)
                     /locus_tag="PLS35_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNI17928.1"
                     /translation="MMRRQVPHSPRRHRSPGRFFLGLLLLFASLPSTQAQAADIVPSR
                     SVTANGMTVLFLEQHFLPTVEIHALVKVGSAQDPPDKAGLANLTASLLDEGTQTRTSR
                     QIAEQIDFVGGSLEAHAAEDFTTASARVLKKDADLGFALLADVLQHPAFHKQEFERVR
                     TQILGEILSDDDDPGNVAMKAFHQLIFHGHPYSWPAHGAEDTLNKITVADVQQFHARE
                     YLPNQTILVIVGDLTQEQAATLVQTHFGSWKKGAPSPYQLKKPTPIDRKMVQLIEKDL
                     TQSTIVLGHTGISRTNPDYYAVTVMNYILGAGGFSSRLMDSIRDKQGLAYGIMSQFDS
                     RLLPGAFYISLQTRTEVTNQAITGVLTEIKGIRDAPVTDQEINEAKSFIVGSFPLRVD
                     SSAKLANVLAQVEFYNLGLDYFTNYPKAIEKVTKDDVLRVAKQYLDPQHYALVVVGSI
                     AKAKVKQ"
     gene            complement(3162..4556)
                     /locus_tag="PLS35_04405"
     CDS             complement(3162..4556)
                     /locus_tag="PLS35_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNI17929.1"
                     /translation="MPKLSTSHRSSLLRHSVFACSLLSLLLFTSQSSTAHAVEPKEFT
                     LSNDMKVILVEVPKAPVATVQVWYKVGSRNEVMGRAGLSHMLEHMMFKGTAKYPKGTF
                     SRLIRKNGGMDNAFTSQDFTAYFENLAADRVTLALELEADRMQGLILDANEFKTEREV
                     VKEERRLRNEDDPQGALVEALFAQAFMSHPYHWPVIGWFSDLDAMSLDDLQRHYDTYY
                     SPNNATLLVVGDIKAETLLPVIARLFEPIPKGPSPKALTVTEGAQHGERRFLLKREAQ
                     VPFVMMGYRVPNYTSDDSYALNVLESILSHGKSARLYQSLVYEQKTALAVGADYGLMQ
                     ADPGLFYFYAVVKPGEKVEAVEDAVLKEIQRLQTEPPSELELQRAKNQIEAAHIFEQD
                     SNFRQAMLLGEAETIGAGWRKVSQFVERTRAVTPQDVQRVASQYLAADMRTTGTLIPL
                     PPQTQSASPAQPHQ"
     gene            complement(4571..5659)
                     /gene="rlmN"
                     /locus_tag="PLS35_04410"
     CDS             complement(4571..5659)
                     /gene="rlmN"
                     /locus_tag="PLS35_04410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNI17930.1"
                     /translation="MLSHTATRHNLLALTESDMAAFVASLGWPAYRASQILRWLYQER
                     VRTFADMSNLSQKDREHLTSTCSIERATDVHIFASQDGTKKFVLTLTDGKQIECVLIP
                     DEDRLTLCLSTQVGCTLDCGFCLTGTMGLTRNLRAHEILDQVLTAQDHLEADQRLTNL
                     VFMGMGEPLANLDAVADAVTRLTNQTWGLGLSGHRITISTAGLASRIKEVAPLKVNLA
                     ISLNATTDELRHQIMPAANRLHSLQALLAACRDYPLAERDRLTFEYVLLADVNDRPED
                     ATRLIKLLRGLRCKVNLIAFNPFPGSPYRRPSDAAIDQFQNILRRGHVDAYLRRSRGR
                     DVLGACGQLGRIEMPQVPVTLTQIQTRC"
     gene            5838..8003
                     /locus_tag="PLS35_04415"
     CDS             5838..8003
                     /locus_tag="PLS35_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNI17931.1"
                     /translation="MTAIAESPRPLPVLPPLCVSAEDCFRSALAVNDRTGSPAQREQA
                     TALKIDQLRSVMELFPSTPWAPRARVVLGVLLIEREPAEATKLLRAAQPELPVIDDYF
                     RLWIAESLSKQNEPIQAAELLETIPKIVPDSNLIAKAAYRTGEAWYSANVCFRAVDWL
                     ERAVALADKDQAAPMALSHQADCHLRENRLPEARTALKQLWLRYPQSPEARDAKTRLE
                     GGLGGESWLPTAEEYLIRAQAFLGLSMQAEAVEELRRFLSLAPGHARRHEARLKLGVA
                     YVRLKQYDQARETFRGLVADRVQESAEATVWLARVYLRQSLGDKLIELARSAAQGPLA
                     GDQRAMVHLFAGVWLEDQGKFDEAIGMFRQVAKLGDSASQRAEGLWRAGWAQYRTARY
                     KDAAETFRAVVELHVNGFEPQAMYWAARAEEHGKNANVGDQYARVCQRHAFSYYCQLA
                     ARRTSIPANQPAVGSVERPAGEEIERLPENRRPEIEKHAVYQRGIELKILGFAQEAAR
                     ELGALTEQYSRDQDVLLAFSTMLSEVGAYHPALRVAKVHFREKLERSGLPTAPALWTV
                     AYPTGLVPTIAAQGVTGVDPYLAAAIIREESQYDEKAVSVVGAVGLMQLMPVTANAVA
                     QRYGFPTVGREDLFDQETNIRLGVRYLGQLIEQYSGNLAHAVAAYNAGPIAVNNWIAV
                     HRGRDQDEFVELIPYQETRLYVKRVLRSYGEYRRLHGAS"
     gene            8098..8352
                     /gene="zapB"
                     /locus_tag="PLS35_04420"
     CDS             8098..8352
                     /gene="zapB"
                     /locus_tag="PLS35_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNI17932.1"
                     /translation="MTLDRLDALEIRIRDLVKLVQDLKRKNTSLEDELRLARERVAVR
                     DDENRRWEQERLDIRSRIEKVLGEIDLLECLDEPKEVAFD"
     gene            8345..8641
                     /locus_tag="PLS35_04425"
     CDS             8345..8641
                     /locus_tag="PLS35_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNI17933.1"
                     /translation="MTKTIDVEIYGQRYTVNGEADESYVKQLAEIVDKQMKQVAAGMR
                     SATPAKLAVLAAFNLAHELMESERRFRQDEADADRRVASLMESIDQQMPSILSR"
     gene            9189..10766
                     /gene="rny"
                     /locus_tag="PLS35_04430"
     CDS             9189..10766
                     /gene="rny"
                     /locus_tag="PLS35_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNI17934.1"
                     /translation="MVPISLSVVAYIFIGLVGAVLGAGLYEVLRRRSAITRRAEAEDQ
                     STQIVQSAQREAENLVKEAKLEAKDLVFQARIELEKEQKAKLAEVSNVERRVSQREEG
                     LDRKVSLLEKRDQDVLKREQDLLKREEVLSQKDAACAQALKQHREALERVAGLTAEEA
                     KRQLIQEMDSQARLEAAGLAKRLLEEAKENADREAREIIASSIQRVTRDYVNEATISV
                     VPIANDAMKGRIIGREGRNIRAIEAATGIDLIIDETPEAVIISGFDPLRREIAKVSLE
                     RLMHDGRIHPTRIEEIVEKVKVDIEKLMIEEAEKVIFEVGLSDFHPELVKVLGRLKYR
                     TSYGQNNLYHAREAAYICGIMASELKLDVKLAKRGALLHDIGKAVSHEEEGPHAMLGA
                     EIAKKYGENAKVVNAIAAHHEQVEPICPETVLVAAAEALSAARPGARREALESYVKRL
                     EKLESLATVHKGVQKAYAIQAGREIRVIVKQEDLSDPECFQLSRDLAKKIEQELTYPG
                     QIKVTVIRESRFVDFAK"
     gene            10777..11556
                     /locus_tag="PLS35_04435"
     CDS             10777..11556
                     /locus_tag="PLS35_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNI17935.1"
                     /translation="MKVLMIGDIMGEPGRRSVARLLPKLIANHSIDVVVGNGENVAGG
                     FGITPDLVDDLFDLGVSVITTGNHAWDKKEILDVFPREPRLLRPANYPAGVPGRGSYI
                     FTTPGGESLGVLHLMGRAFMPTIDCPFQVAKREVERLKSQVSAIVVDMHAEASSEKMA
                     MGHYLDGLVTVVAGTHTHVQTADEQILPKGTAYITDIGMTGPLHSVIGIKKELAIEKF
                     LTGMPRRFEVASGPTVFCAVLIDLDATLGKAVSIERIRVMD"
     gene            11594..12952
                     /gene="xseA"
                     /locus_tag="PLS35_04440"
     CDS             11594..12952
                     /gene="xseA"
                     /locus_tag="PLS35_04440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNI17936.1"
                     /translation="MTGHNLALPLLLSVSDVTRLIRESLEEQFQDVWIEGEISNLRAP
                     SSGHLYFTLKDSQSQLRGVLFRSGALRLRFALQEGLSVIARGRISVYEARGEYQLIVD
                     SLDPKGVGAFQLAFEQLKERLSQEGLFDEARKRRLPSFPSTVGVVTSLTGAAIRDIVA
                     VLRRRCPTVNILIAPVPVQGEGAGASIAEAIDVLSEMPQVEVLIVGRGGGASEDLWAF
                     NEEAVVRAIARSRVPVVSAVGHEIDVTLSDFAADHRAPTPSAAAEAVVPVLAEIMERL
                     DELARRLRRLVDTMLQMQRHRFERSLSVLGETRFRIQAQAQRLDELQDGLTRALTAQL
                     TVLQRELVERRHLLLAQGPRNRIERFLVVIPQLCKRLEQEARRGLMSRRQSVAMQMAA
                     LDALSPLAILQRGFSIVHTISNRKVLRRASEVTVGEAVEVTLAEGQLVCQVEKVMPSS
                     IS"
     gene            13012..13260
                     /gene="xseB"
                     /locus_tag="PLS35_04445"
     CDS             13012..13260
                     /gene="xseB"
                     /locus_tag="PLS35_04445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNI17937.1"
                     /translation="MAAVKFEYAMARLETIVAELEKGDLPLDDSLKIFEEGIRLSKTC
                     LKMLEDAERKVEILVQEKDGKKRIQAFSPGEDELENPQ"
     gene            13325..14080
                     /locus_tag="PLS35_04450"
     CDS             13325..14080
                     /locus_tag="PLS35_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNI17938.1"
                     /translation="MTQKPRPERERLDRALVSRGLVSSREDAARLILAGLVRVDGIVV
                     DKAAKPTMPGALVEVTGPGSPYVGRGGEKLAGALNQFQVDPSGMVCFDVGCSTGGFTD
                     CLLQRGATRVYAVDVGYGQFEWRLRQDPRVVLMERTNVRYLEPGAIAEPIDLIVIDVS
                     FISLTLVLPAVVSHLAASGSIITLIKPQFEVGKGLVGRGGIVRDDALRESTVEKVVSC
                     AAQLGLQSAGRMDSPIEGRKGNREFLAWFQRKA"
     gene            14240..15163
                     /locus_tag="PLS35_04455"
     CDS             14240..15163
                     /locus_tag="PLS35_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI17939.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLLQEGHDVVVVDNLVTGKRKNVPKA
                     AQFYKLDIENPKLERVFRNERPSVVFHLAAQMNVRRSVEDPLFDAQVNVLGTLNVLEQ
                     ASKHGARKVIFSSSGGAIYGEQLAFPAPETHVTQPLSPYGISKLCGEHYLSYYHRMSG
                     IQVVSLRYANVYGPRQDPEGEAGVVAIFIQKMLRGEQAVVNGNGRQTRDFVFVEDVVE
                     ANLMAMGPQVEGVYNVGTGIETSVNDLFRIVVDLTKVEFKEVHGPAKRGEQARSVIDC
                     AKLQRTLGWEPKVELREGLRRTVEFFRDGLG"
     gene            complement(15173..15493)
                     /locus_tag="PLS35_04460"
     CDS             complement(15173..15493)
                     /locus_tag="PLS35_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI17940.1"
                     /translation="MSFTITPTELKSRLDKGDKLVLVDVREPWEYAIAKLEGSVLVPL
                     ATLQQSLGKLDRNSEIIAVCHHGMRSADATGFLLQQGFGNVKNLIGGIDAWSVQVDSS
                     VPRY"
     gene            15814..16170
                     /gene="cutA"
                     /locus_tag="PLS35_04465"
     CDS             15814..16170
                     /gene="cutA"
                     /locus_tag="PLS35_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNI17941.1"
                     /translation="MQIALAGGDVDAQGSEVVVFITASSNEEAEKLGRMVVESRLAAC
                     ANIVSGIRSIFRWDNQVTVENECLMIMKTTLQRYSELEAVIRQHHSYTVPEIIALPIV
                     AGSVSYLDWIRSETAK"
     gene            16289..>17307
                     /locus_tag="PLS35_04470"
     CDS             16289..>17307
                     /locus_tag="PLS35_04470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNI17942.1"
                     /translation="MSQQTAETSDAYTVVVFRGSSSKPLRFSFPRKFVRKLLILAAIL
                     IVADLLVVSHYVIRTGEVWQLSAFRAEAMGAREQTAAFSAAIDDLKKRLSAMGEVNQR
                     LRVMLGIDTTKPAGDLANGRGGEDGPLPDGKSSVPGNGPASSGLESRQQVSELRENNQ
                     SDLDFSTESIEEATQQVRESLEALVREATQQEEALQSLTQVAEQRSTQWASTPSIWPV
                     RGWVTSGFGPRVSPFTEKPAWHDGLDIGAQANSPVQAPALGRVVTVAFDSKMGNMVKL
                     DHGYGIETVYGHLAKSLVKEGQRVKRGDVVALVGSTGLSTGPHLHYMVKKNGQALDPT
                     KFILD"
     assembly_gap    17308..17407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17408..19024
                     /locus_tag="PLS35_04475"
     CDS             <17408..19024
                     /locus_tag="PLS35_04475"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNI17943.1"
                     /translation="GIPSEDLIPYGRYKAKIAANFSARVKSRPLGRYILVTAINPTPL
                     GEGKTTTSVGLSMGLCRLGHNAAVTLRQPSLGPVFGIKGGGTGGGRAQVWPMEDINLH
                     FTGDAHAVSASHNLLSAFVDNHLFQGNALKLDPQRIRWPRTLGVSDRALRDIVLNAEA
                     GPRPGQFVITEASEIMAVLALATDQRDLRQRLGRILVGLTESGAMTTAEAFGCAGSMS
                     VLLKDALLPNLVQTLEGTPAFVHAGPFGNIAHGNCSVVSDQLALRCADYVITEAGFGS
                     DLGAEKFFNIKCRTSGLRPDAGVVVATLRALKLHGGGGIVKPGTPLPPGLTEVNPAAL
                     EKGFANLEQHIANVRAHGVPVVVAVNAFKDDSPEELEWVCERALAAGAAAAAVSTHWA
                     DGGKGAEELARAVVKVAAQGSQFRPLYDATWPIKRKIETIATTIYGAAAVSYSPQAEK
                     GIELAARLGFEQLPICMAKTPLSLSHDPALKGRPSGFTVPIQELRILSGAGFLTAVCS
                     GIQLMPGLPKKPAGERIDIDPRSGEIVGLS"
     gene            complement(19031..>19351)
                     /locus_tag="PLS35_04480"
     CDS             complement(19031..>19351)
                     /locus_tag="PLS35_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNI17944.1"
                     /translation="VFSRMQAERERQAKQYRAEGAEEAQKIKSEAEKDREIILAEAYR
                     ESEELRGGGDAKAFKIYADAYRQDPHFFEFTRTMEAYRKTLKDKTTILVSPDSEYFRF
                     LKQR"
     assembly_gap    19352..19451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19452..19726)
                     /locus_tag="PLS35_04485"
     CDS             complement(<19452..19726)
                     /locus_tag="PLS35_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNI17945.1"
                     /translation="MSKQGFAVAFVGIVIGLLILGASPFYIVDVTQNAIVVQLGKPVR
                     NVTEGGLYLKVPFIEEVTYFDKRLLDYDSNAQDVITQDKKTLLLDNFA"
     gene            complement(19723..20760)
                     /gene="hflK"
                     /locus_tag="PLS35_04490"
     CDS             complement(19723..20760)
                     /gene="hflK"
                     /locus_tag="PLS35_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNI17946.1"
                     /translation="MVWDPKDPWSKKGDDLDQAFKQAQGQLRNLLPTGGFRNLLLVAF
                     TVFLIWQSAFIVAPDEEGVVKRFGIPVRVVEPGPHMKIPVVESVLQPKVAKLHRVEIG
                     FRTDRQGRQQMIPQEALMLTGDMNILAIEFIVQYKIKSSREYLFNVADIDDTIGKAAE
                     ASMREVIGKSKIDEALTTGKAQIQHDTQELLQHILDEYKTGVQVAAVQLQDVDPPEAV
                     AAAFKDVTNAKEDREKLINQAQGYRNDITPKAKGEAAQLVNQAKGYAQARLNRAQGET
                     NRFLATLKEYNQSKDIISKRIYIETLEDVLPHIDKFVLDGKGGERALPYLPLDRFSKP
                     APTAATQERTP"
     gene            21125..21949
                     /locus_tag="PLS35_04495"
     CDS             21125..21949
                     /locus_tag="PLS35_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNI17947.1"
                     /translation="MDAQAIRELIKEKYGEAAARAKTGGSSCCGAAPALMNVDPITSN
                     LYSDQECQGVPADAVAASLGCGNPTALAELQDGETVLDLGSGGGIDVLLSANRVGPKG
                     KAYGLDMTDEMLALARENQRTAGVENVEFLKGEIENIPLPDGSVDVIISNCVVNLSGE
                     KERVLAEAFRVLRPGGRLALSDIVVRGAVPSEIRKNLELWAGCVAGALEETQYGDLLR
                     QAGFTEIGIEPTRIYQAGDVKELLIGGDLSSDQLVAEVDGKFMSAFIRATKPVSKS"
     gene            complement(22096..>23118)
                     /locus_tag="PLS35_04500"
     CDS             complement(22096..>23118)
                     /locus_tag="PLS35_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNI17948.1"
                     /translation="YRTAIMWGTAWTVAGAGFATLVAGAMVKTFSNGLLQTGTMLSPS
                     IALAILFGAMLWVLVASRTGLPVSTTHALTGAIVGTGLVAFAGEGLLWAAIGKKIALP
                     LLLSPFLAFGLSLLLHPVMRRAATKWEGSCLCLMPASRALFTVDARGSTRTVFQSTVF
                     GQPVVAVPVQCDRAGLRGLTVGLDTVHWLSSGMASFARGTNDAPKIVAMLLLGSTTAT
                     WPSVWFQFAAFGGVAFAMGLGSYLGGLRVTEMLAEKVTRMEHAEGLSANLTTSTLVLC
                     SGWLGLPVSTTHVSSSAIIGIGLLKGFNAVRWTTVRDMVLAWVITLPAAGCFAALAYV
                     LLANFA"
     assembly_gap    23119..23218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23223..24260)
                     /gene="arsS"
                     /locus_tag="PLS35_04505"
     CDS             complement(23223..24260)
                     /gene="arsS"
                     /locus_tag="PLS35_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNI17949.1"
                     /translation="MALTLVGQHKPLASSAEQLKVLAEIGSCLPFADQLRRSGLSPLH
                     ATGITVLQINVGKLCNQTCRHCHVDAGPDRTERMSRETAEQCIAALAMTDIPTVDITG
                     GAPELNPYFRWLVEQARALNRHVMDRCNLSVLLLPSQADLATFLAAHQVEIVASLPYY
                     RAAQTDAQRGEGIFEKSIEALQLLNSLGYGREGSGLIVNLVHNPVGAFLPAKQEAIEA
                     QFRKELRAKHGVEFNHLYTITNMPVSRFLEFLVESGNYLGYMERLANAYNPAAAGSVM
                     CRYTLSVGWDGMLYDCDFNQMLDLPVQPDAPAHIRDFAPARLHHRQITTRNHCYGCTA
                     GAGSSCGGTVT"
     gene            complement(24312..24668)
                     /locus_tag="PLS35_04510"
     CDS             complement(24312..24668)
                     /locus_tag="PLS35_04510"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNI17950.1"
                     /translation="MPRHGVANLTTPRQCAAILKAIGDETRLRILESLLLGEKCVTDL
                     THVLRYSQPHVSHHLRILRDAGLVEGHRHGKQVCYRVEPTVQRALKNRGEQVLDFGCC
                     ELRFPTSTLLTLQQKH"
     gene            complement(24668..25855)
                     /locus_tag="PLS35_04515"
     CDS             complement(24668..25855)
                     /locus_tag="PLS35_04515"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI17951.1"
                     /translation="MAIDDITQKVSDRYARAASTGEQMCCPTGYNFEDLRSFIPDAVL
                     KISYGCGTPAGLDTVQTGETVLDIGSGGGIDCFEATRRVGPTGHVIGIDMTDTMLEIA
                     RQHAPIVAGNLGFPRPNTEFRKGMADAMPVEDASIDLIISNCVINLAPDKRKVFREMY
                     RVLKPGGRFTISDIVSDQVVPQYMVHDSAKWGDCLSGSLQVQDYIGGMVDVGFRAVHQ
                     IKSGAWQSIDGVHFLSVTLTGYKLPDAVTQEAPLYATLCGPFSAVTDESGQQYRRGVP
                     QPLDAKSAQLLRSAPLRSLFLIGPAPITLAAGDPRWCAILPEQTPCVWQGAYAILTGP
                     IIAAEDDDHHQYYRGVPLEICSKTQHVLTQDAYRPHFTIYHRSANVVDGEAVSCSPEG
                     TCC"
     gene            complement(26139..26615)
                     /locus_tag="PLS35_04520"
     CDS             complement(26139..26615)
                     /locus_tag="PLS35_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNI17952.1"
                     /translation="MSDRLASLAMILGLALTVSACNSMGSPSAGTAATEKSLYDRLGG
                     KTAITAVVDDFVGRVAGDARINGKFANANIPRLKSMLVDQICQASGGPCTYTGRDMKS
                     THAGMGVSSSDFDALVGDLVATLNKFKVPEREKNELLGALGPMKGDIVEKPMASMQ"
     gene            26839..27444
                     /locus_tag="PLS35_04525"
     CDS             26839..27444
                     /locus_tag="PLS35_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI17953.1"
                     /translation="MCDIQTVANAPSIHEQEWAGLLARIAVGDQSALAELYDASSAKV
                     FGLAMKILGDHAAAEETTLDVYTQVWRRISTYDAERGTPGSWLMTLAKHRAIDRFRSS
                     YLERGRQVPLEQAAELPGCGDTPEQYSAGLERQRLVQDALATLSAEQRQAIALAYYWG
                     MSQSEIAEQLKLPLGTVKTRIRLGMIKLREVLAPHGEGLLS"
     gene            27441..28025
                     /locus_tag="PLS35_04530"
     CDS             27441..28025
                     /locus_tag="PLS35_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNI17954.1"
                     /translation="MTDPRREEDVTELAALYALEALGDDAQRQFARSLETASASTKDE
                     VASFQDVVQELAFSGPAIAPPASLKARLMARIAQEPQEQAEGTGFSFVRSRDVSWQEL
                     APGLSMKVLFHDAAGARTTMLLRLAPGGTLIGHRHPQVEEFYVLEGSCLCAGELLQVG
                     DYHRAEAGTVHSVTSSEQGCLALVMTSSKNEPIR"
BASE COUNT         5755 a         7375 c         8816 g         5930 t
ORIGIN      
        1 tctatttagg ggatgccgtg accttgcacg gtcatagcga gcacgcacac taggtcgagt
       61 ggtctataaa cggaggagag gacacacaat ggatgcagca gcagcagcgt tggtcggcat
      121 ggggttggcg gcggcaggtt ttgccggagc cggcgtggga atcgggtaca tcttcggaaa
      181 aatgattgag gccgtggccc gccagccgga agccgaaggt cgcgtcggga agtacatgtg
      241 gatcggtttc gcgttggtcg aagctatcgc cttgtacggg ctggtcattg cgttcatcat
      301 catgggcttg cgcaagtaag cgcaccgacg gtcgagccct gatgctgggg acaggcccgg
      361 ctcagaaccc tcaactcatt gggtaacaga ctatgcctca atttgaatca catttctttt
      421 cgtcgttgat cttctgggag attctctcct tcgggattct cttcttcttg ctctacaagt
      481 acgcgttccc gagcttgttg ggcatgctgg aggagcgtga gaaaaagatc aaggacagtc
      541 tcgatcaagc cgaacgccat cgttcggagg ccgagcgcaa gctcaaggaa tatgaagcca
      601 agctggccac cgcagccaaa gacgcggagg cactcttggc tcaggccaaa gagcgcgcgg
      661 cacggttgat ggaagagaac gaacagcgca tgacggcgga tgcagagcgg atcaagggcg
      721 acgcgactcg tgagatcgag accgaacgtc gtaaggccat tcaggacatc cgctcgcaaa
      781 cgacggattt agcgatgatg gttgcggaga aagtggtcgg gcgagcattg accgacgggg
      841 atcaccgccg gttggccgat gaagccctgg acgcacttgc gaagacctac cagagccgga
      901 actaagttcc tgacgccggg ggggtgagac ttacacctcc ctggcgatat ccttccaggt
      961 ccaacgtcac gaagcgatag cctatctgtt tcagactctc gacgagctgt tcgcggcacc
     1021 ccgcttccaa caatttcggc agctcctcta cggccaactc aatcctcgcc aggtccccgt
     1081 agtcgcgtac ccggcagtgg cgaaaacctc cctggatcaa agccgcttcc gcctgctcca
     1141 cgcgcgagag cgtgcttcga gtgatcgtga tgcctctcgg gacgcgtgag gagaggcagg
     1201 cagccgcagg cttgtcccaa ttggagaggt gcagttcgcg ggcagcttcg cgaatgtcgg
     1261 cttttgtgaa tccggcttcc aataatgggc tgcgcacccc atgttcccgg gcggccttca
     1321 gaccggggcg atcgtctcct aagtcgtcaa cgttggtgcc atccacgata cacgccgtcg
     1381 catcttcgct tctcaattta cccaagaggg aatagagatc ggtcttgcag tggtagcagc
     1441 gggtggcatc attgcggacg aactgcggga tttccagttg atccgtctcg accacccgat
     1501 ggcgcgctcc gatttccgtc gcgagttgct gagtcagcgc gagttccgat gcgggaagcg
     1561 tgggtgagac ggcggtgact ccggtggcct gcattcccaa ttcatcatgc gccagtttga
     1621 ggaccaacgt actgtcgatg cctccggaaa aggccacgat caccgagccc atctctcgaa
     1681 ggaggtgccg tgcccgtgaa atcttttcga tcagtggtgt gctggagacc atggggagtc
     1741 cgacgtgttg cgcggtcgtc gagtcgggca aggaaggagc tattgtttca ctttggcttt
     1801 cgcaatcgaa ccgacgacga ccaaggcgta atgttgaggg tcgagatact gtttcgccac
     1861 gcgaagaaca tcgtccttcg tgaccttttc gatggctttg ggatagttcg taaaataatc
     1921 caatcccaga ttatagaact ccacctgtgc gaggacgttg gccagcttgg cgctggaatc
     1981 gacccgcaga gggaagctgc cgacgatgaa ggacttcgct tcattgattt cctgatccgt
     2041 gaccggggcg tcgcgtatcc ctttgatttc agtcaggacc ccggtgatgg cttgattcgt
     2101 gacctcggtt ctggtctgga ggctgatgta gaacgcgccc ggcagcaggc gggaatcgaa
     2161 ttggctcatg atgccgtacg ccagtccctg cttgtctcga atggagtcca tgagccgtga
     2221 agagaatccc ccggcgccca aaatgtagtt catgaccgtg acggcgtagt agtccgggtt
     2281 cgtccggctg atgccggtat gccccagcac aatcgtcgat tgcgtgagat ccttttcgat
     2341 caactgcacc attttccggt cgatcggcgt cggcttttta agctggtagg gcgatggcgc
     2401 gcccttcttc cacgagccaa agtgggtttg taccaatgtg gctgcttgtt cctgcgtcag
     2461 gtccccgaca atgaccagaa tcgtctgatt cggtaaatat tcccgcgcgt ggaactgctg
     2521 gacatcggcc acggtgattt tgttcagcgt gtcctcggcg ccatgcgccg gccagctata
     2581 gggatggcca tggaaaatca attgatgaaa ggccttcatg gccacattgc cgggatcgtc
     2641 gtcatcgctg agaatttcac cgaggatctg ggtgcgaacc cgttcgaatt cctgtttatg
     2701 gaaagcggga tgctgcaaca catcggcgag gagggcaaac ccaaggtccg cgtctttttt
     2761 cagcacgcgc gcagatgcgg tggtgaaatc ttccgccgca tgggcttcaa gagagcctcc
     2821 cacgaaatcg atctgctcag caatctgccg ggatgttctg gtttgagtgc cttcgtccag
     2881 gaggctggct gtgagatttg ccaggcccgc tttatccggc ggatcttgag ccgaccccac
     2941 cttcaccaag gcatgaattt ccaccgtggg aagaaagtgt tgttcaagaa acaggacggt
     3001 catgccgttt gccgtcacgg agcggctcgg aacgatgtcg gcggcctgtg cttgtgtgct
     3061 cggcagagag gcaaacagca ggaggagtcc gagaaagaac cggcctggtg accggtggcg
     3121 tcggggtgaa tgcggaacct gtcgtcgcat catctatgtg ctcactggtg cggttgggcc
     3181 ggcgacgccg attgagtttg cggcgggagc ggaatcaatg tcccggtggt gcgcatatcg
     3241 gcggccagat attgcgaggc aacacgctgg acgtcctgtg gcgtgacggc acgggtacgt
     3301 tcgacgaact gactgacttt acgccatcca gccccgatcg tttccgcttc tcccagcagc
     3361 atggcttggc ggaagttgga atcctgttcg aaaatgtggg cggcttcgat ttggtttttg
     3421 gcgcgttgga gttcgagctc actgggtggc tccgtttgca gccgctgaat ctctttcagt
     3481 accgcgtcct ccacagcttc gactttctcg cctggtttca cgaccgcata aaaataaaat
     3541 aagccaggat cggcctgcat caagccgtaa tccgcgccca cagccaaggc ggtcttctgt
     3601 tcatagacga gactttggta cagccgcgcg ctctttccgt gggacaaaat cgactcgagc
     3661 acgttcaacg cataggaatc gtcgctcgtg tagttaggca cccggtaccc catcatgaca
     3721 aagggtactt gggcttcgcg tttcaacaga aaccgtcgtt cgccgtgctg cgcaccttcg
     3781 gtgacagtca gggctttagg cgaaggacct ttgggaatcg gttcaaagag tctggcaatg
     3841 acgggaagga gcgtctcagc cttgatgtcg ccgacgacga gcagggtcgc gttgttcggc
     3901 gaataatacg tatcataatg gcgctgaaga tcgtccaggc tcatggcatc gaggtcggaa
     3961 aaccacccga tcaccggcca atgatacgga tggctcatga aggcttgtgc gaagagcgct
     4021 tcgaccagcg cgccctgggg atcatcttca ttgcgaagac gacgttcttc cttgacgact
     4081 tcccgctcgg ttttgaattc gttcgcatca agaatcagtc cctgcatccg gtctgcttca
     4141 agctcaaggg ccagggtcac gcgatcggcg gccagatttt caaagtacgc ggtaaagtcc
     4201 tgactggtga aggcgttgtc cattccgccg tttttgcgga tgaggcggga aaacgtcccc
     4261 ttgggatatt tcgcggtgcc tttgaacatc atatgttcca gcatgtgcga gagcccagcg
     4321 cggcccatca cctcattgcg ggaaccaacc ttgtaccaga cctgcacagt cgccacggga
     4381 gccttcggga cctccacgag gatgaccttc atgtcgttgg acagcgtgaa ctctttcggc
     4441 tcgacggcat gggcggtgga gctctggctg gtgaacagca ggaggcttag aagcgaacaa
     4501 gcaaatactg aatggcgaag cagggatgat ctgtgggatg tgctcagctt aggcatgtac
     4561 gaagatcatg ctaacaacgg gtctgaatct gtgtcaaggt aaccgggact tgcggcatct
     4621 cgattcgacc caactggccg caggcgccca ggacatcgcg accgcgactg cgccgcaggt
     4681 acgcatccac gtggccccgg cgcaggatgt tctgaaactg gtcaatagcg gcatccgacg
     4741 ggcgtcgata gggactgccg ggaaagggat tgaaggcgat caagttgacc ttgcagcgaa
     4801 ggccgcgcag cagtttgatc aatcgggtcg catcctcagg ccgatcgttc acgtccgcca
     4861 gcaggacgta ttcaaaggta aggcgatcgc gttcagccag gggatagtcg cggcaggcgg
     4921 caagcagggc ttggagcgaa tggagccggt tcgcggccgg catgatctgg tggcgcaact
     4981 catcggtggt ggcgtttaat gaaatcgcca ggttgacctt caagggggcc acctctttga
     5041 ttcgcgacgc gagacccgca gtggaaatgg tgatccggtg acccgacaat ccgagtcccc
     5101 atgtttgatt cgtcaggcgt gtgaccgcgt cggctacggc gtcgaggttg gccagcggct
     5161 cgcccattcc catgaacacc aggttcgtca ggcgttggtc ggcttccaaa tggtcctggg
     5221 ctgtcaggac ctggtcgagg atttcatggg cgcggagatt gcgcgtgagc cccattgtgc
     5281 ccgtcaggca gaatccgcaa tcgagcgtgc agccgacctg cgtggacagg cacagtgtga
     5341 ggcggtcttc gtcgggaatg aggacgcatt caatctgctt gccgtccgtc agcgtgagca
     5401 caaatttctt cgtgccgtcc tgcgaggcaa agatgtggac atccgtggct cgctcgatac
     5461 tgcaggtgga ggtgagatgc tcacggtcct tctgcgagag gttgctcatg tcggcaaagg
     5521 tgcggacccg ttcctgatag agccagcgaa ggatttggga ggcgcgatag gccggccagc
     5581 ccagcgaggc cacgaatgcg gccatgtccg attcggtcaa cgccagcagg ttatgacgtg
     5641 tcgcagtgtg tgagagcatg acggagccta gcacagggaa aaaacttgtg acaagcaagg
     5701 cgaacctggt cgcgtttcta tggcgttacg ctataatgat gtcggttcac cttctgctca
     5761 ggggcattgt gcgcggactt ccttggcgga ccagtatagc ggcattcgcc gtgtgtcttt
     5821 ttctgtcgag tgtattcttg acggcgatag cggagtcccc tcgtccgttg ccggtgcttc
     5881 ctcctctctg tgtctccgct gaagattgct ttcgttccgc cctcgccgtc aatgatcgaa
     5941 ccggttcccc cgcccagcgg gagcaggcga cggcgctcaa aatcgaccag ctgcgatccg
     6001 tgatggagtt gtttccgtcg actccatggg ctccacgagc tcgggtcgtg ttaggcgtcc
     6061 tgctcatcga gcgagaaccg gctgaggcca ccaaacttct ccgggccgct cagccggaat
     6121 tgcccgtgat cgacgattat tttcgtcttt ggattgcgga gtcgttgtcg aaacagaacg
     6181 aaccgatcca agccgccgaa ctgctggaaa cgattcccaa gattgtgccg gattcaaacc
     6241 tgatcgcaaa ggcggcctat cgaacaggag aggcctggta cagcgcgaat gtatgtttcc
     6301 gcgccgttga ttggttggag cgtgccgtgg cgctagcgga taaggatcag gcggctccga
     6361 tggccttatc ccaccaggcg gactgtcatc ttcgcgagaa ccggctgccg gaggcccgca
     6421 cggcgctgaa gcagctctgg ctccgttatc cccaatcacc ggaagcccgc gacgcgaaaa
     6481 cgcgattgga aggcggtctg ggaggagagt cttggctgcc gactgccgag gaatatttga
     6541 ttcgagcgca ggcgtttctt ggactctcga tgcaagccga ggccgtggag gagttgcggc
     6601 ggtttctctc gttggctccc gggcatgccc gccggcatga ggctcgcctc aagttggggg
     6661 tcgcctatgt tcggctcaaa caatatgacc aggcccgtga aacattccgc ggcctcgtgg
     6721 ccgaccgagt gcaggaatca gcggaagcca cggtatggtt ggcccgggtg tatttgcgac
     6781 aaagccttgg cgacaaactc atcgaattgg cacgatcggc agcccagggt ccactagccg
     6841 gtgaccaacg ggcgatggtg catctcttcg ccggcgtgtg gttggaggat cagggcaagt
     6901 tcgatgaggc gatcggcatg ttccggcagg tcgcgaagct gggggattca gccagccaac
     6961 gggctgaagg gctctggcgg gccgggtggg cgcaatatcg gaccgcccgg tataaggatg
     7021 ccgcggagac cttccgtgcc gtcgtggagt tgcacgtcaa tggctttgaa cctcaggcga
     7081 tgtattgggc agcgagggcc gaggagcatg gaaagaacgc gaacgtcgga gaccaatacg
     7141 cgcgggtctg tcagcgccat gcctttagtt attattgcca gctcgctgcg cgacgaacgt
     7201 cgataccggc gaaccagccg gcagtgggat cggttgaacg gccggccggg gaagagatcg
     7261 aacggttgcc ggaaaatcgg cgtcccgaaa tcgaaaagca cgcggtgtat cagcggggaa
     7321 tcgagctcaa aatcctgggg tttgcccaag aggcggcgcg ggagctggga gcgctgaccg
     7381 agcagtacag tcgcgatcag gatgtgctgc tagccttttc aacgatgctc agtgaagtgg
     7441 gggcgtatca tccggcctta cgcgtagcga aagtgcattt tcgggaaaag ttagagcgaa
     7501 gtggattgcc gacggcaccg gccctatgga ctgtcgccta tcccactggc ctcgttccaa
     7561 cgatcgccgc ccagggcgtg accggcgtcg atccctatct cgcagccgct atcatccggg
     7621 aggaaagcca atacgatgaa aaggcggtct cagtggtggg ggcggttggc ttgatgcagc
     7681 tcatgccggt gacagccaat gcggtcgcgc aacggtacgg attcccgacg gtcggccggg
     7741 aagacttgtt tgaccaggag acgaatatcc gcctcggagt gcggtatctg gggcaattga
     7801 ttgagcagta cagcggcaat cttgcccatg cggtcgcggc ctacaatgcc gggccgattg
     7861 ccgtgaacaa ttggatcgct gtacatcggg gacgtgatca agacgagttc gtggagctca
     7921 ttccgtacca ggaaacgcga ttgtatgtga agcgggtctt gcgcagttac ggagaatatc
     7981 gccggctcca cggcgcgtca tgacgagacc ggttttcttg acaagggggc atgaactttc
     8041 tatatttcgc ccccattaac catcgtgtgt gaccatcact ttgaatgagg aaataccatg
     8101 actttagatc gtctcgatgc gctggaaatt cggattcgtg atctcgtgaa gctggtgcag
     8161 gatctcaagc ggaagaacac gtcgttggag gacgagcttc gcctggcgcg tgagcgggtc
     8221 gcggtgcgcg atgatgaaaa ccggcgctgg gagcaggaac gtctcgatat ccggtcccgg
     8281 atcgaaaagg tgctcggcga aattgatttg ctggagtgcc tggatgaacc caaggaggtg
     8341 gcgtttgact aaaaccattg acgtggagat ctatggccag cggtataccg tgaatggtga
     8401 ggccgacgaa tcctacgtga agcaactcgc cgagattgtt gataaacaaa tgaaacaggt
     8461 ggcggccggt atgcggtcgg ccaccccggc caaattggcc gtgcttgccg catttaatct
     8521 tgcccatgag ttaatggaat cggagcggag gtttcggcag gatgaagccg atgccgaccg
     8581 ccgcgtagcc tccctcatgg agtcgatcga tcaacagatg ccgtccatct tgtcgcgttg
     8641 agtttcacct tgctttcact gcactccttt gttatcgttt cttcgttgtg ttgacctgag
     8701 ggtcaacaag agcaggagag gaaaggacgc ttcgaataag gacggaaaat ctgacgacgg
     8761 tatttgagct ctaaacatat ggaatcgctg gtttttgtgc tgctgttcag tgtggtcgtg
     8821 attgcatgga tgagtcaggg ggctcggccc tatgtgcccg tgcgacaggt gtgtcatcaa
     8881 ccaacgccga tgttggagaa ccagaggctc accaggatct gggggcattc cccgtggctt
     8941 gaatatgcaa cagttcgaac aaggcggctc ggctctgttc gtgcacgagc tcacgtaaga
     9001 aacgatctag gtcgaggccg accacgcctc tacttggact agcgacggcg attccgcact
     9061 tcgtgttcga ctggctcaag tagtcaggtt ccttcctgtc cagctattcc ttcacttcct
     9121 gctcccttta ccactggctg cgattatttg cagggtgaca gtgtgcctgt cccactgccg
     9181 taagggaggt ggttcccatt tctctcagcg ttgtagcgta catttttatc gggttagttg
     9241 gagcggtctt gggcgccggt ctgtatgaag tgttgcgccg ccggtctgcc attacacgac
     9301 gggccgaggc tgaggaccag tccacgcaga tcgtgcagtc ggcccagcgt gaagccgaaa
     9361 acctcgtgaa agaggccaag cttgaagcca aggacctcgt gtttcaagca cgaatcgagc
     9421 tggaaaagga acaaaaagcg aagcttgccg aggtctcgaa tgtggagcgg cgggtgtctc
     9481 agcgcgaaga ggggctggac aggaaagtca gcctgcttga gaagcgtgat caggatgtgc
     9541 tcaagcggga gcaggatctc ctgaagcggg aagaggtgct ctcgcagaaa gatgcagcct
     9601 gtgcacaggc gctcaagcag catcgcgagg cgttggagcg ggtggccgga ctcactgccg
     9661 aagaagccaa gcggcaattg attcaggaga tggatagtca ggctcgcttg gaggctgctg
     9721 gtttagccaa gcggttgctc gaggaagcga aagaaaacgc ggatcgtgaa gcgcgggaga
     9781 tcatcgcctc ttcaattcag cgcgtgaccc gtgattatgt gaacgaggcc acgatttcgg
     9841 tggtgccgat cgccaatgat gccatgaagg gccggattat cggccgtgaa gggcgaaaca
     9901 ttcgcgctat cgaggcggcc acgggtatcg atctcattat cgatgaaacg cctgaagccg
     9961 tgattatctc gggcttcgac ccccttcgcc gcgaaattgc caaggtgtcg ctcgagcggc
    10021 tcatgcacga tggccggatt catccgaccc gcattgagga aatcgtcgaa aaagtaaagg
    10081 tcgatatcga gaagctgatg atcgaagagg cggaaaaggt catcttcgaa gtcggcttgt
    10141 cggatttcca tcccgagttg gtcaaggtgc tgggacggct caaatatcga acgagctacg
    10201 gacagaacaa tttgtaccat gcgcgggagg cggcctacat ttgcgggatc atggcctccg
    10261 agttgaaact cgacgtgaaa ctcgctaagc gaggggcgct gttgcacgat atcggcaaag
    10321 cggtgagtca cgaggaagag gggccgcatg ccatgctcgg cgcagagatc gctaagaaat
    10381 acggcgaaaa cgccaaggtg gtcaatgcca ttgcggcgca ccatgagcag gtggagccta
    10441 tttgccccga gaccgtgctg gtcgcggcgg cggaagcgct ctcggcggcg cgtcccggcg
    10501 ctcgtcgtga agcgttggag tcctacgtga agcggttaga aaagctagag tcgctggcaa
    10561 ccgttcacaa gggcgtacag aaagcctatg cgattcaggc cggacgggaa attcgcgtga
    10621 tcgtcaagca agaagatctc tcggatccgg aatgtttcca gctctcccgg gatctggcca
    10681 agaaaatcga acaggaactg acgtacccgg ggcaaatcaa ggtcacggtg atccgggaga
    10741 gccggttcgt cgattttgcg aagtgagcga cagcgtatga aagtcctgat gatcggagat
    10801 atcatgggcg agcccggacg acggtccgtg gctcgcctcc taccgaagct cattgccaat
    10861 cattccatcg atgtggtcgt cggcaacggc gagaacgtgg ccggtggatt cggcattacg
    10921 cctgatctgg tggatgatct ttttgacctc ggggtctcgg tcattacgac agggaaccac
    10981 gcctgggaca agaaagaaat tttagacgtg tttcctcggg aaccgcgtct tctgaggcct
    11041 gcgaactatc ccgcgggggt gcccgggcgg ggtagctaca tcttcacgac gccgggcggg
    11101 gagtcgctgg gggttctcca tctcatggga cgggcgttca tgccgaccat cgattgcccg
    11161 tttcaagttg ccaaacggga agtcgagcga ctcaagtcgc aggtgtcggc catcgtggtt
    11221 gatatgcatg ccgaagcctc gtctgagaag atggcgatgg gacactacct tgatgggctg
    11281 gtgacggtgg tggcgggaac tcatacgcac gtgcagacgg cggacgagca gattcttccc
    11341 aagggaacgg cctacatcac cgacataggc atgaccggtc cgttacattc ggtgatcggt
    11401 atcaaaaaag agctcgcgat tgaaaagttt ctcacgggga tgccgcgccg gtttgaggtg
    11461 gcgtcgggcc cgacggtgtt ctgcgccgtc ctcattgacc tggatgccac cctcggcaaa
    11521 gcggtgtcga tcgaacgtat ccgagtgatg gattgatctg tccgctgact tattcccttc
    11581 cgctcggtga gccatgaccg ggcacaatct tgcgctgcca ttgctgctgt ccgtgtccga
    11641 tgtgacgcgg ctcatccggg agtctctgga agagcaattt caggatgtct ggatcgaggg
    11701 cgaaatctcg aatcttcgtg cgccgtcttc cggccacctc tacttcacgc tgaaagatag
    11761 ccagagccaa cttcgcggcg tgttgtttcg gtccggagcc ttgcggctgc ggtttgcctt
    11821 gcaggaaggc ttgtctgtca ttgcgcgcgg gcgcatctcc gtctacgagg cccgtggcga
    11881 gtatcaattg attgtcgact cgctcgaccc caagggcgtc ggcgcgtttc agctggcatt
    11941 cgagcagctc aaggagcggt tgtctcagga gggattgttc gatgaggcgc gcaaacggag
    12001 gttgccatca tttccttcga cggtcggggt ggtcacctcg ctgaccggtg cagcgattcg
    12061 ggatattgta gccgtgcttc gccgccgttg ccccaccgtc aatattctca ttgccccggt
    12121 tcctgttcag ggcgaaggcg ccggggcgtc cattgccgag gcgatagacg tcttgagtga
    12181 aatgccgcag gtagaggtgc tgatcgtcgg tcgtggcggc ggcgcgtcgg aagatttgtg
    12241 ggcgttcaac gaagaggcgg tggttcgcgc cattgctcga tccagggttc cggtggtctc
    12301 ggctgtgggg catgaaatcg atgtgacgct ctcggacttt gccgcggacc accgtgctcc
    12361 cacgccctct gcggcggctg aggcggtggt acccgtattg gccgagatca tggaacggtt
    12421 ggatgaattg gcccggcgtt tgcgccgcct tgtggatacc atgctgcaga tgcagcggca
    12481 ccggtttgag cgatcgctca gcgtgttggg cgagacacgg ttccggatac aggcgcaggc
    12541 tcagcggctc gatgaattgc aggatggatt aacgagggcc ttgaccgcac agctgacggt
    12601 gctgcagcgc gaactggtgg agcgccgaca cctcttgctt gcgcagggac cgcgcaatcg
    12661 gattgagcgg ttcttggtgg tcattcctca gctctgcaag cgcttagagc aggaggctcg
    12721 ccgcgggctc atgtccaggc ggcagtcggt ggccatgcag atggccgcgc tcgatgcgct
    12781 cagtccgtta gccattttac agcgcgggtt cagcatcgtt cacacgattt ctaatcggaa
    12841 ggtcttgcgc cgggcctccg aggtgacagt cggagaggcc gttgaggtga cactcgccga
    12901 aggacaattg gtttgtcagg tcgagaaggt catgccctcg tcgatatctt gacccgacca
    12961 ggagcgcagg caataatgtt ttgattgacg gatgagcaaa agggagttgt cgtggctgcc
    13021 gtaaaattcg aatatgcgat ggcgagattg gagacgatcg tcgccgaact tgaaaaggga
    13081 gaccttcctc tcgacgattc actgaagatt ttcgaggaag gcattcggtt gtcgaagacc
    13141 tgtctgaaga tgctggagga cgccgaacgg aaggtggaga ttctggtgca agagaaagac
    13201 ggcaaaaagc gcattcaagc gttttctccc ggtgaggatg agttggagaa cccacagtag
    13261 tcgtttggct ctacgcgggc cacgatgtgg cctgcccatc atcgtcgaac ctcctctcac
    13321 acgtatgact cagaaacctc gcccagaacg ggaacgcctg gatcgtgccc tggtcagccg
    13381 tgggcttgta tccagtcgtg aggatgcagc cagattgatc ctggcggggc tggttcgtgt
    13441 ggatggcatc gtcgtcgaca aggcggcgaa accgacaatg ccgggtgcgc tggttgaagt
    13501 gaccgggccc ggatcaccct acgtggggcg tggaggagaa aagctggccg gcgcgctgaa
    13561 tcagtttcag gtcgatccga gtggaatggt ctgtttcgat gtgggatgtt cgacgggagg
    13621 gtttaccgat tgtctgctgc agcgaggcgc cacccgtgtg tatgccgtcg atgtggggta
    13681 cggtcaattt gagtggcgtc ttcggcagga tccgcgagtg gttcttatgg agcggaccaa
    13741 cgtgcggtac ctcgagccgg gtgccatcgc ggagccgatc gatctgatcg tgatcgacgt
    13801 ctcattcatt tccctcacgc ttgtattgcc tgcggtcgtt tcccatcttg ccgcgtccgg
    13861 ttccattatc acgctgatca agccgcaatt tgaggtgggg aagggactgg ttggccgggg
    13921 agggattgtg cgggacgacg cattgcgaga gtcgacggtg gaaaaggtgg tgtcttgtgc
    13981 ggcgcaactc ggcttgcagt cggcaggccg catggattct ccgatcgagg gccgcaaagg
    14041 caatcgcgaa ttcctcgcat ggtttcagcg gaaggcctga atccggcccc gtaggccgtc
    14101 gcaggatggt cctggccaat ggccttctgg caggggcagg acgaaaacac tttgaggaag
    14161 cggacagaag gtgtaggctg agttccgata cagtccggat gctgctgtta cggctgatga
    14221 aggagaggcg aggagacgta tgaaagtctt ggtgaccgga ggggctggat ttatcggatc
    14281 acacgttgtg gatcggttgc ttcaagaggg gcacgatgtc gtggtggtgg ataatctcgt
    14341 aacgggcaag cgcaaaaacg tgcccaaggc ggcgcagttc tataagctgg acatcgaaaa
    14401 tcccaaactg gagcgggttt tccgcaatga gcggccttct gtggtctttc acctggcggc
    14461 tcagatgaat gtgcggcggt ccgtcgaaga tccgctattc gacgcccagg tcaatgtgct
    14521 ggggacgttg aatgtcttgg agcaggcatc caagcatggt gcgcgaaagg tcattttttc
    14581 gtcatccggc ggggcgatct atggagagca attagccttt cccgcaccgg aaacgcatgt
    14641 cacgcaaccg ctgtctccct atgggatcag caaattgtgc ggggagcact acctgtccta
    14701 ctaccaccgg atgagcggca tacaggtggt gagcttgcgg tatgccaatg tgtacggacc
    14761 tcgccaggat cctgaaggcg aagcgggcgt cgtggccatt ttcattcaga aaatgctgcg
    14821 cggggagcag gcagtcgtga atgggaatgg tcgccagacc cgtgattttg tgtttgtgga
    14881 agacgtcgtc gaagcaaatt taatggcgat gggccctcag gtagagggtg tgtacaatgt
    14941 ggggaccggc attgagactt cggtcaatga cctgttccgg atcgtcgtgg acctgaccaa
    15001 ggtggaattt aaagaagtgc atggtccggc caagcgcggt gagcaagcga gaagcgtcat
    15061 tgactgcgca aaactccagc ggacactcgg atgggagccg aaggtcgagt tgcgagaagg
    15121 cttgcgacga acggtggaat tttttcgaga tggactcggg tagcctcccg ggctagtacc
    15181 gaggcacgga cgaatctacc tggacggacc aggcgtcgat acctccgatt aagtttttta
    15241 cgttcccaaa cccttgctgt aagagaaatc ccgttgcatc cgcgcttctc atcccgtggt
    15301 ggcaaaccgc gataatttct gagttgcgat ctagtttccc cagggactgt tggagcgtgg
    15361 ccagtggtac caggactgac ccttccaact tggcaatggc gtattcccag ggctcacgaa
    15421 catcaacgag cacaagtttg tcacctttat ccaacctcga cttgagttct gtgggtgtaa
    15481 tagtaaagct catcaatata cctccctctg gactgaatgg tcggatcata aggaggcaga
    15541 aaaggggtgt caaccctggt gaggccttgc tggggagtgt ctctcaaggt tcgacgcgtt
    15601 cagtccgaag aagagcagcg gtatcagata agccggacgg ttggttcaac agtatggggc
    15661 cgggaaaatc ctgcatcgca ggatggccgt tttttgtttc tctgtgcaat tcctgcccag
    15721 attggtagga gcgttgtgct ggaaaatgtg ggaaaatatc ccgtaagtgg ttgatttata
    15781 tcagcatgtc tgtgattggc gacaggtatg ggcatgcaaa ttgctttagc agggggagac
    15841 gtggacgcgc aaggttccga ggtcgtggta ttcatcactg cctcgtcaaa tgaggaagca
    15901 gagaaactag gacgaatggt cgtcgaatcc agacttgccg cctgcgccaa catcgtgagc
    15961 gggattcgct caatttttcg atgggacaat caggtcactg tcgagaatga atgcctgatg
    16021 atcatgaaaa cgaccctgca gcggtattct gagctggagg ccgtgattcg gcagcaccac
    16081 agctatacgg tcccagaaat catcgcgttg ccgatcgtcg cgggctcggt ttcgtacctg
    16141 gactggatca ggagtgaaac tgctaagtag ctgattctaa tatgtaaaat tttgaaaact
    16201 aagggttgac caccgggctt gagatgggta cactgcaaaa gtcggtggtt gcggtttgaa
    16261 agtgaatccg tgaaggggag accagcctat gagccaacaa acggctgaaa caagtgatgc
    16321 ctacactgtc gtggtttttc gaggatcatc ctccaagcca ctccggttta gtttcccacg
    16381 gaaattcgtc cgcaagctct tgatcctggc tgcaattttg attgtggccg atctgctcgt
    16441 tgtatcccat tatgtgattc gaaccggaga agtctggcag ttatccgctt ttcgagctga
    16501 ggccatgggt gctcgggagc aaactgcagc cttttctgca gccattgatg atttgaagaa
    16561 gagactgtcg gccatgggag aggtcaatca acggcttcgg gtcatgctgg gaattgatac
    16621 gaccaagcct gctggcgacc ttgccaatgg gcgaggtggt gaagatggtc ccttgccgga
    16681 tggaaagagc agtgtaccag ggaatggacc ggcgtcctca ggactggaat ctcggcagca
    16741 ggtatcggag ctgcgtgaga ataaccagtc cgacctggat ttctcgaccg agagcattga
    16801 agaggcgacc cagcaggttc gtgaaagctt agaagccctc gttcgggaag ccacgcagca
    16861 ggaagaagct ctccaaagcc tgacacaggt tgccgagcag cgctcgaccc aatgggcgtc
    16921 caccccgtcg atctggccgg ttcgcggttg ggtcacgtcg ggttttgggc ccagagtctc
    16981 gccgttcact gagaagccag cctggcacga cgggctcgat atcggggccc aggcgaactc
    17041 ccccgtacag gctccggccc ttggtcgtgt ggtcaccgtg gcatttgatt ccaaaatggg
    17101 caatatggtg aagctggatc acgggtatgg cattgaaacc gtgtacgggc atcttgccaa
    17161 gtccctggtg aaagaggggc agcgcgtgaa gcgcggcgat gtcgtcgcgc tcgtaggcag
    17221 caccggcctt tcgaccggtc cacatctcca ctacatggtg aagaagaacg gccaggcgct
    17281 tgatccgaca aagtttatcc ttgattannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnggg attccttcgg aagacctcat cccttatggc cggtacaaag ccaagatcgc
    17461 tgccaacttt tctgcgcgcg tgaagagcag gccgctcggg cggtatatcc tcgtgacggc
    17521 cattaacccc actccgcttg gcgaaggtaa gacgacaacc tccgtaggtt tgagcatggg
    17581 cctctgccga ttgggccata atgcggcagt gacgttacgg caaccttccc tcgggccggt
    17641 ctttggtatc aaaggcggcg ggaccggtgg agggcgcgcc caggtctggc cgatggagga
    17701 tatcaatctg catttcaccg gggatgcgca tgccgtctct gcaagccata atctgttgtc
    17761 cgcattcgtg gataaccatc tgtttcaggg caatgcgctc aaactggatc cgcaacgaat
    17821 cagatggccg cgcaccctgg gggtgagtga tcgagcgctt cgcgacatcg tattgaatgc
    17881 ggaagcaggg ccgcgaccag ggcagtttgt catcaccgag gcatcggaga tcatggcggt
    17941 gttggcattg gccacggatc agcgtgatct acgccaacgc ctcggtagaa ttctggtcgg
    18001 cctgacagag agcggtgcga tgaccaccgc cgaggcattc ggttgtgccg gttcaatgtc
    18061 cgtattactc aaggatgccc tcttaccgaa tctggtgcag accttggagg gcacccccgc
    18121 gtttgttcat gccgggccct tcggcaacat cgctcatggg aattgttccg tcgtctcgga
    18181 tcagttggcg ttgcgatgtg cggattatgt cattaccgaa gccggtttcg gcagcgattt
    18241 aggtgcggaa aagttcttca acatcaaatg ccgtacatcc ggtctccggc ccgatgccgg
    18301 agtggttgtg gcgacgttgc gtgccctcaa gctgcatggg ggaggcggga ttgtaaaacc
    18361 tggaacaccg ctgccgccag ggttgacgga agtgaatccg gccgcgctcg aaaaagggtt
    18421 tgccaatctc gaacagcata ttgccaatgt gcgcgcgcat ggagtgcctg tggtggtggc
    18481 tgtcaatgca ttcaaagatg attcacctga ggagttggag tgggtctgtg aacgcgcgct
    18541 ggctgcagga gcggcggcgg ccgctgtgtc gactcattgg gccgatggtg ggaaaggggc
    18601 ggaggaactg gctagggccg ttgtcaaagt cgctgcacaa ggatcacagt tcagacctct
    18661 ttatgatgcc acttggccca tcaagaggaa gatcgagacc attgccacca cgatctacgg
    18721 cgcagccgcg gtgtcgtact cgcctcaggc ggaaaagggt atagagttgg cggcgcgatt
    18781 agggttcgag cagcttccta tctgtatggc gaagacgcca ttgtcgcttt cccacgaccc
    18841 cgcgcttaaa gggcggccat ccgggtttac ggtgcccatc caagaattgc gaatcctgtc
    18901 cggtgcgggc tttctcacag cggtatgctc gggcattcaa ctgatgccgg gcctgcccaa
    18961 aaagccggcg ggcgagcgca tcgatatcga tccgcggtcg ggcgagatcg tcgggctgtc
    19021 gtgatgtcgg ttagcgctgc ttgagaaacc ggaagtactc agagtcggga ctgacgagga
    19081 tggtggtctt gtctttgagg gttttgcgat aggcttccat cgtgcgggtg aactcgaaga
    19141 aatgggggtc ctggcggtac gcgtccgcat agattttgaa cgctttggca tcgccgccgc
    19201 cacgaagttc ttctgattcg cgataggctt cggcaaggat aatttcccgg tctttttcgg
    19261 cttccgactt gatcttctgc gcctcttccg cgccttccgc ccgatattgc ttggcttgcc
    19321 gttcccgctc cgcctgcatg cgtgaaaaga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn ngcaaagttg tcgagcagga gagtcttttt gtcctgcgtg atcacgtcct
    19501 gggcgttgga atcataatcc agcagccgct tatcgaaata ggtaacttct tcaatgaacg
    19561 gcaccttcag gtacagcccg ccttcagtga cattgcggac cggcttgccg agctggacca
    19621 caatggcatt ctgggtgaca tcgacaatgt agaaggggga cgcgccaaga atcagcaaac
    19681 cgatgacgat gccgacaaac gcgaccgcaa atccctgctt gctcatggcg tacgctcctg
    19741 cgtcgcagcg gtgggagccg gtttgctgaa acggtctaac ggaaggtacg gcagggctcg
    19801 ctctccacct ttgccatcca ggacgaattt gtcgatgtgg ggcagcacgt cttcaagcgt
    19861 ctcgatgtag atgcgcttgc tgatgatgtc tttcgactgg ttatattctt tgagggtggc
    19921 gaggaaacga ttggtttcac cctgggcgcg attcaatctc gcctgcgcgt accccttggc
    19981 ttgattcacc aattgggctg cctctccctt ggctttggga gtgatgtcgt ttcgatagcc
    20041 ctgcgcctgg ttgatcagtt tttcacgatc ttccttggca ttggtcacgt ccttaaacgc
    20101 cgcggccacg gcttccgggg gatcgacatc ctgcaactgg acggcggcca cctgtacgcc
    20161 ggttttgtat tcatccagaa tgtgctggag cagctcctgg gtgtcatgct ggatctgggc
    20221 tttgccggtg gtcagggctt cgtcgatttt actcttcccg atgacctcac gcatggacgc
    20281 ttcagccgcc ttgccgatcg tatcgtcgat gtcggcaaca ttgaacagat actcgcgtga
    20341 acttttgatc ttatattgga caatgaattc gatggccaga atgttcatat ccccggtcag
    20401 catcaaggct tcctgcggaa tcatctgctg acggccctgc cggtcggtac gaaatccaat
    20461 ttccacgcgg tgcaatttcg ccactttggg ctgcagcacg ctttcgacca ccggaatttt
    20521 catgtgtggt cccggttcga ccactcgaac cggaatgccg aagcgtttga cgaccccttc
    20581 ttcatcgggg gccacgatga acgcgctttg ccagatgagg aagaccgtga aggcgaccag
    20641 gaggagattg cgaaatccgc cggtgggcag caggttgcgg agttggcctt gagcctgctt
    20701 gaaggcctga tccaaatcgt cgcccttttt gctccaaggg tctttggggt cccagaccat
    20761 cgcgtggctc aattctgtaa gtggtggcaa cgatgctcgc gggtcaccat agcaaagtga
    20821 gcacctgtga gcaacaccta taacggcgac aacagccagc tgttttactc atcaaaatgt
    20881 attgatgtag agtggtggca agacaccgtg atcatgaatc cggtccggac ggtggaactg
    20941 tttcatgcgc tggcatggcc agaccagact tgaaatttgg acgaactgaa gggtggcgaa
    21001 cggtgcgtct gtgagctgat agattgcctt gaggccgggc ggtcacagct ctcgttccat
    21061 tgcgatgttg agtgtaagtg tgtctgatat atcaataagt gtgatgcaaa gaggagatgc
    21121 cgaaatggat gcccaagcga ttcgagagtt aatcaaggaa aagtatggtg aagcagcggc
    21181 acgggcgaaa accggaggca gttcctgctg cggggctgcc ccggcgttaa tgaatgtcga
    21241 cccgattaca tccaacctgt attcagatca ggagtgccag ggggtaccgg cagatgccgt
    21301 tgcagcgtcg ctcggttgcg ggaatcccac ggcactggcg gaacttcagg acggcgaaac
    21361 tgtgttggac ctcggatcag gcgggggaat cgatgtgttg ctctccgcca accgagtcgg
    21421 accgaaaggc aaggcgtatg gacttgatat gactgacgag atgctggcgt tggccagaga
    21481 aaatcagcgt acggccggtg tggagaatgt cgagtttctg aagggcgaaa tcgaaaacat
    21541 cccgctgccc gacgggtcgg tggatgtgat catcagcaac tgcgtggtga atttgtccgg
    21601 ggaaaaagaa cgtgtgttgg cggaagcgtt tcgcgttctg cgaccgggag ggcgcttggc
    21661 gttgtccgat attgtcgtgc gaggggcggt accatcggag atccgaaaga atctggaatt
    21721 gtgggctggt tgcgtggcgg gggcgctgga agagacgcag tatggggatt tgttgcggca
    21781 ggcgggtttt accgagatcg gaatcgaacc gacgcgcatc taccaggccg gagacgtgaa
    21841 ggagttgttg atcggcggag atctctccag tgaccaactt gtggctgaag tggacggcaa
    21901 gttcatgagt gcattcatcc gggctacgaa accggtaagt aagagctagg gcgcgagcag
    21961 ggaccacaaa ggagcagtcg catagagctt gcgcaaacag tcctcgcgcc agcgggagac
    22021 acgaagcggc tgtcgttttg tgaacaatac ttcactctct ggggttggca gcggtttcct
    22081 ggtgcagaag cccggctaag cgaagttggc aaggagtacg taggccagag cggcgaaaca
    22141 tccagcggcc ggcaaagtga tcacccaggc caacaccatg tcccgcacgg ttgtccagcg
    22201 gacagcgttg aagcctttga ggagaccgat cccgataatg gcggaactgc tgacgtgtgt
    22261 ggtcgacacc ggcaatccta accagccgga acagaggacg agcgtggagg tagtgaggtt
    22321 cgcagacaat ccttctgcat gttccatacg cgtgaccttt tcggctaaca tctcggtgac
    22381 tcggagcccg cccaagtagc tccctaaacc catggcgaat gccacgccgc cgaaggccgc
    22441 gaactgaaac cagacgctgg gccacgtggc tgtcgtgctg ccgagcagga gcatcgcgac
    22501 aattttgggt gcgtcgttgg tgccgcgggc aaaggaggcc atcccgctgg acagccaatg
    22561 gaccgtatcg aggcccaccg tcaggcctcg cagccctgcg cggtcacatt gcaccggcac
    22621 cgccacgacc ggctggccaa acaccgtgct ctgaaacacc gttcgtgtgc tgccccgtgc
    22681 gtcaacggta aagagtgccc gcgaggccgg catgaggcac aggcaggatc cttcccattt
    22741 tgtcgccgct cgtcgcatga caggatgtag gaggagggag agaccaaagg ccagaaaggg
    22801 actgagcagc aagggcaacg cgattttttt gccgatggcg gcccacaata gtccctcacc
    22861 cgcaaacgcc accagaccgg ttcccacgat ggcgccggtg agcgcatggg ttgtcgaaac
    22921 gggaagtccc gttcttgatg ccacgagcac ccagagcatt gcaccaaaca gaatcgcgag
    22981 ggcgatggag ggtgaaagca tggtgccggt ttgcaataga ccgttgctga aggtcttcac
    23041 catcgctccg gcgaccaggg tggcgaatcc ggctccggcc acggtccagg ccgttcccca
    23101 catgatcgcc gtgcggtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    23221 gattaggtca ccgtcccacc gcacgacgat cctgcaccgg ccgtgcagcc gtaacaatga
    23281 ttgcgagtgg taatctgccg gtgatgaagt cgggcagggg caaagtcccg gatgtgcgca
    23341 ggggcatcgg gctgcaccgg aaggtccagc atctggttga agtcgcaatc gtagagcatg
    23401 ccgtcccagc cgaccgagag tgtatagcgg cacatcacgc tgccggcggc ggcgggattg
    23461 tacgcattcg caagccgttc catgtacccc agatagttcc cgctctccac caggaattcg
    23521 aggaagcggc tgaccggcat gttggtgatg gtatagaggt ggttgaattc cacgccatgc
    23581 ttggcgcgga gctccttccg gaattgcgct tcgatggcct cctgtttggc tggtaggaag
    23641 gcgccgaccg gattgtgcac gagattcacg atcaatccgc tgccttcgcg tccgtagccg
    23701 aggctattga ggagctgcag agcttcaatg gacttctcga aaatgccttc gccgcgttgc
    23761 gcatcagttt gcgccgcacg gtaatacgga agtgacgcga caatctccac ctggtgcgcc
    23821 gcgagaaagg tcgccagatc cgcctgcgag ggcaacagca gcaccgacag attgcagcga
    23881 tccatgacat ggcgattcag ggcacgggcc tgctcgacca accagcggaa ataggggttc
    23941 agttccggtg cgccacctgt gatgtcgacg gtaggaatgt cggtcatggc caaggccgcg
    24001 atgcactgct cggctgtctc ccgggacatg cgttccgttc ggtcgggccc cgcgtctacg
    24061 tgacaatggc ggcaggtttg attgcaaagt tttcccacgt tgatttggag caccgtgatg
    24121 ccggtcgcgt ggagcggcga caggccgctt cgccgaagct ggtccgcaaa tggcaagcag
    24181 gacccgatct ctgccagtac cttcagctgc tcggcagagg aagcgagcgg tttatgttgg
    24241 ccgacgagtg tcagtgccat ggagagttat tccaagtact atggagtatg ttcatcgcct
    24301 gcattccggt ttcaatgttt ttgctgcagc gtgagtaagg tcgaggtggg aaatcgcaat
    24361 tcgcaacatc cgaagtccag tacctgctca ccacgatttt ttaacgcgcg ctgcaccgtc
    24421 ggttccacac ggtaacagac ttgtttgccg tgccgatgtc cttccaccaa tccggcgtca
    24481 cgcagaatcc gcagatgatg ggagacatgc ggctgggaat agcgaagaac gtgcgtgaga
    24541 tcggtcacac atttttcgcc caggaggagg gactctaata tgcggagcct ggtctcatcc
    24601 ccgatggcct tcaagatcgc ggcacactga cggggggtcg tgagattggc gacgccgtgg
    24661 cgtggcatca gcagcaagtc ccttcaggcg aacaactgac cgcttcgccg tctacgacat
    24721 tcgcgcttcg atggtagatg gtgaaatgag gtcggtaggc gtcctgtgtc aggacgtgct
    24781 gggttttaga gcagatttcc agcggcactc cccgatagta ttgatgatga tcgtcgtcct
    24841 cggcggcgat gatgggccct gtgagaatgg cataggcgcc ctgccagacg cagggtgtct
    24901 gctccggaag gatagcgcac cagcgcgggt cgcccgccgc gagggtgatc ggggccggac
    24961 cgatcagaaa cagcgagcgc agcggcgcac tccgcaatag ctgtgcagac ttcgcatcga
    25021 ggggctgcgg cacgccgcgt cggtattgct ggcccgattc gtcagtcacg gcggaaaacg
    25081 gtccgcagag ggtcgcatac agcggggcct cttgtgtgac cgcgtcgggg agtttgtatc
    25141 cggtcagggt gacggagaga aaatgcacgc cgtcgatcga ctgccaagcg ccggacttga
    25201 tttggtgcac ggcgcgaaac cctacatcga ccatcccgcc gatgtagtcc tggacctgca
    25261 aggatccgga gaggcagtca ccccacttgg cggaatcgtg gaccatgtat tgtggaacga
    25321 cttgatccga cacgatatcg gagatcgtga agcgcccgcc gggttttagg acgcggtaca
    25381 tctcgcgaaa gaccttgcgc ttgtcaggcg ccaggttgat cacgcagttg gagatgatga
    25441 ggtcgatgct ggcgtcctcc accggcatcg catcggccat gccctttcga aactcagtgt
    25501 tcggtcgggg gaacccgaga ttgccagcga cgatgggggc atgctggcga gcaatctcca
    25561 gcatggtatc agtcatgtcg atgccaatca cgtgcccggt ggggccgacg cgacgcgtgg
    25621 cttcgaagca atcaattcct ccaccagatc cgatatccag cacggtttca ccggtctgca
    25681 ccgtgtctaa ccctgccgga gtgccgcacc cgtaggaaat tttcagaacg gcatccggaa
    25741 tgaacgagcg cagatcttca aagttgtacc ctgtcggaca gcacatctgc tcgccggtcg
    25801 aggcggcccg ggcatagcgg tcgctgactt tctgggtgat gtcgtctatt gccataaggt
    25861 tcctttgcgt tcgaggcaac tatagagatg tctctatcta tcaacccttc gtatccgagg
    25921 tcaatccggt gaacgggttg gctaaccggt gggacggcgg gttgtaggaa ttaagggggt
    25981 ggcgcgttgt atgtgacgtc tgatcgagag atgagcgtaa gaattgatgc gaatcgcgta
    26041 aagagcatga agcaacacga accgtcgcct tcgtccccag gccctggcag ggacagccga
    26101 atcgctgccc ctgccagtgt gcgagcgcgg cgcgttgcct actgcatcga ggccatcggc
    26161 ttttcgacga tatccccttt catcgggccg agcgcaccca gcaactcgtt cttttcacgt
    26221 tccggcacct taaacttgtt cagcgtcgcg acgagatcgc cgaccagcgc atcgaaatcg
    26281 ctgctgctga cgcccatgcc cgcgtgggtg ctcttcatgt cgcggccggt gtaggtgcag
    26341 ggaccgcccg acgcctggca aatctgatcg accagcatgg atttcaggcg ggggatgttg
    26401 gcgttggcga atttgccgtt gatgcgggcg tcgcctgcga cacgtccgac aaagtcatcg
    26461 acgacggcgg tgatggcggt tttgccgccc aaacggtcat agagcgattt ctccgttgcg
    26521 gcagtcccag ccgacggtga gcccatgctg ttgcaggctg agaccgttag cgcgagaccg
    26581 agaatcatgg caagcgacgc aagacgatca gacatgatgg ttcctcctgg ttaagatctg
    26641 tgtggcgagc cggattcatg gtttccccaa ccggctgctc tgtctgtgat tacgaaggcc
    26701 gagcgcaatt ggatctacgg cgcgacaaaa agtgccgttg cgccccgctg gtcccggtgc
    26761 tacagttccg cctgaatcgg ccggagattg aacatccaaa cgaatgggtg cgctcgtaga
    26821 acgtcacgag gacaccccat gtgtgacatc cagaccgtgg ccaacgcacc atcgattcac
    26881 gaacaggagt gggcgggact actcgcgcgt attgccgtgg gagatcaatc cgcactggcc
    26941 gagctgtatg atgccagtag tgcaaaggtc ttcggcctgg cgatgaagat tctcggcgat
    27001 catgcggcag ccgaagagac gaccctggat gtctacacgc aggtctggcg acgcatctcg
    27061 acgtatgacg cggagcgggg cacgccgggt agttggttga tgacgctggc caagcatcgc
    27121 gccatcgacc ggtttcgcag cagttattta gagcggggcc ggcaggtgcc gctcgagcaa
    27181 gccgccgaat taccgggatg cggcgatacg ccggagcaat acagcgcggg gttggaacgg
    27241 caacgattgg tgcaggacgc gctcgcgacg ctctccgcgg agcagcgtca ggcgattgcc
    27301 ctggcctatt actggggcat gagtcagagt gaaattgccg agcagttgaa gctcccgctg
    27361 gggacggtga agacgcggat aaggctgggc atgatcaagt tgcgcgaagt gctcgcgccc
    27421 cacggggaag ggttgttgtc atgaccgatc ctcgacgcga agaagacgtg acggaactcg
    27481 cggcgctcta tgccttggag gcgcttggcg acgacgcaca gcggcagttc gcacgttcgc
    27541 tggagacagc ctcggcgtcc acgaaggacg aggttgcgtc atttcaagac gtggtgcagg
    27601 agctggcgtt cagcggtcct gccattgcgc cgccggcttc cctcaaggcg cggctcatgg
    27661 cgcgaattgc acaggaaccg caggagcagg ctgaaggcac cgggttcagt ttcgtcagaa
    27721 gccgggacgt gtcgtggcag gaattggcac cgggtctctc gatgaaggtg ttgtttcacg
    27781 atgccgccgg tgcacgcacg accatgttac tcaggctggc gcccggtggc acgctgatcg
    27841 gccaccgcca cccgcaagtc gaagagttct atgtgctgga ggggagctgt ctctgcgccg
    27901 gggagctcct gcaagtcggg gattaccatc gggcggaggc gggcaccgtc cattccgtca
    27961 cgtccagtga gcagggttgc ttggcactgg tgatgacctc ctcaaagaac gaaccgatcc
    28021 gatagcgaag cgatcgctat gtctgctctc ccttagttcg gaagaacacc tgccaataca
    28081 gggccaggtt caccagcagc acgaggatgc ccagcagaag ctgaatctcg cgcgtcagtg
    28141 ccgccgggta caacagggat ggaagatagc ggccga
//
LOCUS       DAOQEW010000024        28162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_382_length_28162_cov_8.101007, whole genome shotgun sequence.
ACCESSION   DAOQEW010000024 DAOQEW010000000
VERSION     DAOQEW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28162
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_28162_cov_8.101007"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLS35_04535"
     CDS             complement(<1..991)
                     /locus_tag="PLS35_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNI17955.1"
                     /translation="MPFSPRTKKILSAIAVGVAVYALVTGLRMTRWFEVVELKAFDHL
                     VRRYADPAKADSSLVLLAIDESSLEAFGRWPWPRDRYGYVVRYLKRAGAKAVVFDVMF
                     FEPDDNAEEFDQSFADDVKAAGNVFLPMLFQSEAAPVPAGLQDHATVKAERSDSGPVQ
                     AAAHPGVKLPIPVLAQQARGLGVINLSADADGPTRRLPLLGQVQGNLVPHLSLAVARY
                     VLGGSELAMRDDRLQIGDSSVPLDADGNLLIKWHGSLEQTYHAKRYSIGRVLQAFAQQ
                     EKGDRPDLDASLFKDKIVFVAGTAAGLYDLRVTPFSSATPGVLIHMAALDNLLH"
     gene            complement(1100..3532)
                     /locus_tag="PLS35_04540"
     CDS             complement(1100..3532)
                     /locus_tag="PLS35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNI17956.1"
                     /translation="MQSEKLQFEACKVLEWAKLLDLLAGYAQSEVGAEQCRSMELERD
                     VAQARLRQEETSDMLRLRAGVDPFPVLRFPDVTEWLRRVVKGACLEAHELRDIALVLE
                     LVVDVQRYLLRHQPDAPTVGAMAEQLGPVTEYRRLTVALNGAIDPDGSIRESASPELH
                     RLIQRANDFKQQMRHRLEQILHSRRYEEVLQERYFAQREGRYVVPIKAEMQGRMPGIV
                     HDVSSSGATLFLEPRELVDLNNSIKVVDLDIEREVRRILRELSALVAPHQPALAGSVT
                     LLGRLDAISAKAGLSQRLQGSPPRLNDQGRILLTQARHPFLVVSKDQVVSNDLAFDET
                     VRVLIISGPNTGGKTVILKLLGLFSLMVRCGLHLPCAPESEMAIFPSVYADIGDAQDL
                     ARDLSSFSAHITQMVQLLNEVSDGAGSEHDREPVPPGRALVLLDEPVTSTDPAEGAAL
                     ASALLCRLAAAGVKVVATTHYSLLKGLAQDRPGFANASVEFNLDTLSPTYRLIQGQPG
                     GSAAIEIAGRLGMDQSLLEDARARLQGDNRLLETLLSDLQDKQRRLNDDLVAATAARQ
                     GAEQASREAQELLTFLQESERDERQGLKKKLSQEFQRARAAVQATMDDLKRDQKLLKA
                     KEAKARLVELEESVRREAGDSEEPIPIDQLTIGDAVEVIGLGMTGTLLENPQGKKRVR
                     LKVGEGEILANVASLAGVSKARQPQAPAAVKTSVVSPRRTSQTLAPEDIQETLDVRGQ
                     AADDALDVVVAGLDRAILGGSPFVRIIHGHGTGRLRSALRDYLKDSPYVVAFRGGDRA
                     EGGDGVTIVQLR"
     gene            3748..4755
                     /gene="gap"
                     /locus_tag="PLS35_04545"
     CDS             3748..4755
                     /gene="gap"
                     /locus_tag="PLS35_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNI17957.1"
                     /translation="MTTRIGINGFGRIGRNVLRASLGDPSLEFVAINDLTDAKTLAYL
                     LKYDSVHGTLDASVEAKDDQLIIDGRAIKVLAVRDPKELPWKALGVDIVVESTGHFTD
                     REGAGKHLSAGAKTVIISAPAKDPDATVVLGVNEQVFDAKAHHIVSNASCTTNCLAPV
                     AKVLLENFGIKHGVMTTIHSYTNDQQLLDLPHKDLRRARAAGMSMIPTSTGAAKALHL
                     VIPQLKGKLDGLAIRVPTPNVSLVDLTVETEKDCDVAGVNAAFKKAAEGPMKNVLAYS
                     EAPIVSIDLKDDAHSAIVDAPLTAVIDKRLVKVTAWYDNEWGYSCRVRDLIKYIVAKS
                     A"
     gene            4852..6051
                     /locus_tag="PLS35_04550"
     CDS             4852..6051
                     /locus_tag="PLS35_04550"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNI17958.1"
                     /translation="MNIRKRIIEDLQLRGKRVIIRADYNVPLDDSLQITDDTRIRSTL
                     PTINRAVDEGAKVILCSHLGRPKGKFDPKFSLAPVAKRLQRLLGKEVIFAPDCIGSAV
                     EGLVAKMQPGDVMLLENLRFHPEEEKNDEGFSKALAALADVYINDAFGAAHRAHASTV
                     GITKYIPEAAAGYLLKKEIEYLEGAVENPVRPFVAILGGAKVSGKIGVIENLGKKVDK
                     VIIGGGMAFTFLKAMGLEIGQSLVENDMLDFAKGVQDHAFASGVKFYLPVDCVVAAGR
                     EPGAETKIVPVQEIPKGWYGLDIGPASVKLFSEAVQDAKTILWNGPMGMFEVDAFARG
                     TLAMAHSVANAYALTIVGGGETALAIHRAGESESISFISTGGGAALELLEGKTLPGLA
                     ALPNRAA"
     gene            6087..6866
                     /gene="tpiA"
                     /locus_tag="PLS35_04555"
     CDS             6087..6866
                     /gene="tpiA"
                     /locus_tag="PLS35_04555"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNI17959.1"
                     /translation="MRTRFLIGNWKMNKTATEAGAFVRRLTQELPRSEGIQVGFAPPF
                     TALHAAHEALGSSPDFLLGAQDVYWEDKGAFTGEVSGTMLKDLGCQFVLVGHSERRQY
                     FGEQDSWTNKKVLAALRNGIQPVLCVGETLHDRDTNQTDQVIQRQVRAGLAGIEGQHL
                     ESMVIAYEPVWAIGTGRAASPEQAVPVHRLIRQTINELSGAEAGARVRILYGGSVTPH
                     NIAEFLASEEIDGALVGGACLDPVSFATLAKVAIGSKPTTA"
     gene            6877..7230
                     /gene="secG"
                     /locus_tag="PLS35_04560"
     CDS             6877..7230
                     /gene="secG"
                     /locus_tag="PLS35_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNI17960.1"
                     /translation="MYTLLIIIHVIVCLLMIGAILLQSGKGAEIGAAFGGSSQTVFGS
                     RGPANFLSKFTVITAFVFMFTSLSLAILAKDRNFSSTVIDLNKKPAATTPSTTPAETA
                     TPPAKDSHSSTDSSH"
     gene            complement(7471..8385)
                     /locus_tag="PLS35_04565"
     CDS             complement(7471..8385)
                     /locus_tag="PLS35_04565"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNI17961.1"
                     /translation="MLQTSEKIWMDGKFVAWADAQVHVLTHSLHYGLAAFEGIRCYKG
                     TSGSAIFRLPEHVDRLFESAHIGLIEIPYDRKQMTDAIIETVRVNRLEACYIRPLVYI
                     GYGAMGLYPGENPIKVSIAAWKWGTYLGDEALQKGIRARVSSFTRHHVNVSMTRGKIS
                     GYYVNSILAKREVKADGYDEAIMLDPEGYVAEGTGENVFIVRRGVLKTTPLTSILEGI
                     TRNSIIQLAKERNIPVVEERFTRDEMYVAEEVFVTGTAAELTPVTEIDQRRIGTGKPG
                     TITQTLQKAFFDVVRGTDAAHSQWLTPV"
     gene            8596..8910
                     /gene="rplU"
                     /locus_tag="PLS35_04570"
     CDS             8596..8910
                     /gene="rplU"
                     /locus_tag="PLS35_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNI17962.1"
                     /translation="MYAIIETGGKQYRVETGTVLQVESLPGDVGHSVQIDKVRLLNGD
                     SGLVIGQPVVAGARVTAEIIRQGRTRSITVFKKQRRKNYRRTRGHRQGFTQLRVTEIA
                     SK"
     gene            8932..9195
                     /gene="rpmA"
                     /locus_tag="PLS35_04575"
     CDS             8932..9195
                     /gene="rpmA"
                     /locus_tag="PLS35_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNI17963.1"
                     /translation="MATNKGGGSTRNGRDSNPQYLGVKAYGGETIKAGSIIVRQRGTK
                     FFPGLNVGLGRDHTLYAYVSGVVKFEGGRGRQKVSVYPVTAKA"
     gene            9323..10354
                     /gene="obgE"
                     /locus_tag="PLS35_04580"
     CDS             9323..10354
                     /gene="obgE"
                     /locus_tag="PLS35_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNI17964.1"
                     /translation="MSTFVDQVQILVKAGHGGNGACSFRREKFVPRGGPDGGDGGDGG
                     SVIVEATTRLSTLLDLRYQKHYEAENGEVGGGSNCQGRRGADVRIPVPVGTIVLDANT
                     QELLADLTADGESCVIAKGGLGGRGNSRFATSTNRVPTQFEPGTPGEERLLRLDLKLL
                     ADVGLVGYPNAGKSTLIAAVSAARPKIADYPFTTLTPNLGVVRSTGEHSFVIADIPGL
                     IEGAHEGKGLGFQFLRHIERTSLLIHVIDISEWATEEPVASLKVMRHELSAYDEALAA
                     RPFAVVGTKLDIKGEGERLEQLRKYCQRRKIPFFAISAATREGLDECILYMGQQVELL
                     RKTPCETNS"
     gene            10336..11457
                     /gene="proB"
                     /locus_tag="PLS35_04585"
     CDS             10336..11457
                     /gene="proB"
                     /locus_tag="PLS35_04585"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNI17965.1"
                     /translation="MRDELLKQARRVVIKIGSSLVASRESGLSTERIERLASEIAHVR
                     AQGREVLVVSSGAVVSGIKKLELREYPKSLPVKQAAAAVGQSLLMWAYEKAFERLSLR
                     VAQVLLTHEDLADRRRFLNARHTLTTLIKFGVIPIINENDTVAVEEIRVGDNDTLAAQ
                     VAHLVDADLLIILSDVDGLFTADPRKDLQATLIPLIQEITADIEQRAGMSSTFEGTGG
                     MATKVRAAKKVGEYGVATLILNGTRTGLLPDVLSGQPGGSLFLGRERRMNSRKHWIAF
                     TLRPRGHVQLDQGAVDALVRNGKSLLASGIRDITGQFEAGDPVTCAGPDGKECAKGLV
                     NFSSTILTRIKGLKTADIQKIPGLQEYEEVIHRDNLVLL"
     gene            11547..12227
                     /gene="nadD"
                     /locus_tag="PLS35_04590"
     CDS             11547..12227
                     /gene="nadD"
                     /locus_tag="PLS35_04590"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNI17966.1"
                     /translation="MRLGVLGGSFNPIHRCHLSIAQSARRLLCLDQVLFIPTGDPPHK
                     QPDTLASAQHRYRMVQLAIQDEPAFTLTDIEICRSGKSYSIDTVRALQEQYGSDTEIF
                     FIIGLDAFLDLPSWKDAAQLLSLCHFVVISRPATTFLTVASLPLFPEVPKEELLALDT
                     GRLERADVPLKNGRTVTFLRLPPCESSASDIRARVANGRPLANLLPPLVESYILREGL
                     YREDSERF"
     gene            12214..12636
                     /gene="rsfS"
                     /locus_tag="PLS35_04595"
     CDS             12214..12636
                     /gene="rsfS"
                     /locus_tag="PLS35_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNI17967.1"
                     /translation="MSVSETKAKALAVAAAILDKKATDVLILHIAKLTSIADYLVIGS
                     GESERQARAIADHVGDVLKAQGHPPLGIEGASSAKWVVMDFGDVIVHIFQKDIREHYA
                     LERLWGDAGQVKLPEERAVKVTPPARTKARPARTRKRV"
     gene            12638..14038
                     /locus_tag="PLS35_04600"
     CDS             12638..14038
                     /locus_tag="PLS35_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI17968.1"
                     /translation="MFRLLSTIFLVSVGIFLYSYFRELNPGTITVRTSPDAIFELSPV
                     SLVLFSMALGATLVALIVTIKETSHVFMNWRTNRLVRRKEKVDALHRDGTHAFMSKRT
                     ADAVSLFERALVIDPNRTDSLLWLGNIYRSESNFTEAIRLHQQAYRIEERNVEVLLEL
                     AKDLEGARRYEEALQTLQNILRIEPDNLMALIRKRDLQIRLEKWSDALEIQHRLVKAN
                     LPESERRAEANLLLGCMYEVGRQLLERGHPDKARRYFRGAIKKDRTFLPAYIGIGEIL
                     VREGKIKNAVEILKKIYAKTRSVIILHRLEELFLELGEPDEIIRVYQEALQQDPHNAV
                     IQFYLGKLYYRLEMVDEAYDVLSTLDGTQEQLVDYHKIMANLFLRKQHMDEAVGELKK
                     ALGFKKRVVVPYQCTQCHHELAEWSGRCRRCGRWNTYVALPWRDSTKSGADQDGQAAR
                     LPAVPYQGIASPFETV"
     gene            14139..15170
                     /gene="bioB"
                     /locus_tag="PLS35_04605"
     CDS             14139..15170
                     /gene="bioB"
                     /locus_tag="PLS35_04605"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNI17969.1"
                     /translation="MTDFTILADKALRDELLSREECLTVLHTPDTNLLELLQAAFKVR
                     ERHFGKTVRLQMLLNAKSGACQEDCGYCSQSSVSTAPIERYGLLPQDQMVTGARRAAA
                     AKAQRYCIVISGRSPLDREIADIASAVRSIKQEVPIQICCSLGLLNERQAKDLKAAGV
                     DRINHNLNTSEAYHTEICSTHTFQDRLATIRHARTAGLEICSGGIVGMGEGDEDLIDL
                     ALALREVKPDSIPLNMLHPASGTPLEHCTPLTPQRCLKALCLFRFLHPRTELRIAGGR
                     EHNLRSLQPLALYPADSVFVNGYLTTPGQPAAEVWRMIEDLGFDIQVDALPTQGATSS
                     AEPAVGLHS"
     gene            complement(15161..17815)
                     /locus_tag="PLS35_04610"
     CDS             complement(15161..17815)
                     /locus_tag="PLS35_04610"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17970.1"
                     /translation="MSLFHSSLLRVCASLGWTHLSTHPGRTALTMFGVGLGVAATIAV
                     QTANVEVLRSFEESVLTVAGPVTLEVSAGETGLDERLISSVRTVPGVLSARPVLEVGV
                     RVTDGRSPRQSFSILGLDLLEELSPEGGRVVSPANRGEGAGERMPLEDLLARNGLLIG
                     HALASELSLALRAQLALQSGDQHVSVSIIGVMGRRPGGPSPWDRIAVMDIAAVQRTFG
                     LIGRLDRIDVVTTPTSSVEQVAEAIGRVLPPATTVRRPIQRSQQVESMVGAFQLNLSV
                     LSMVGLLVGIFLIYNTVSFTVAQRRREVGILRAIGMSEPMVVAVFLAEAGVFGVVGGL
                     LGGMLGLLLGDVLVALVGRTIHDLYTPLSHSTGTVGFPVGSGRLLVEAVLIGSGVSVL
                     GALGPSVDAGRTIVVAALAPGEYDVAQRVRAASLGVAGGVLLLIALGCALAGPVGGVP
                     IFGYVATFCLLAGLSCLVPILLQSLCRTRELTTLSQAPTLGGAIRHIAREQTSRGMGR
                     NAVTVSAFLVGVAIMVGVLVMIRSFRDTVEIWIDQTVMADFIVAPAGWPHAVRSGSSS
                     PVLPGAWRARLANAPLVSAVDMYRDLRIDLQGRPVSLVSRDLALHAARSRYLFVTGES
                     APILARAAAGEGAILSEVVANHLALGKGDRLSIVTPKGEQSLEVLGVFYDYATDGGKI
                     VIDRSLYQQWWPDDGVTVFPVYVESGADMGKARMAILGTLAQASQGNLMPTVLSNAEL
                     RQEILRIFDRTFTLTYVLEAIAVIIATLGIINTLVTSVVERRRELATLQALGSSRGQV
                     TALILWEAGYLGLLGTGMGLVGGGALALILIRVINRQSFGWTIQISWPVGLMAEVAAL
                     ALVASLVAGFWPARWAARQPLVEGLRYE"
     gene            17914..18249
                     /locus_tag="PLS35_04615"
     CDS             17914..18249
                     /locus_tag="PLS35_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI17971.1"
                     /translation="MALRKRPQAKKSSKTTKAAPAAKKRKPVKLAKASPGRTRRSDGL
                     TPRQKEILKLVSQGNTNRDIARRLDISVRTVEVHRFNLMRRLKVRNVAQLLRQALQQG
                     FLPKNFAFR"
     gene            complement(18292..19218)
                     /locus_tag="PLS35_04620"
     CDS             complement(18292..19218)
                     /locus_tag="PLS35_04620"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HNI17972.1"
                     /translation="MSLSMTIAGVTFPSCLMNASGALCMTREELLALGRSRAGAIVTK
                     SMTVEPRGGNPEPRYYGFPGGSINSMGLPNLGYLAYADLIPELKQFGKPVIASIAGLC
                     EDDFLTMARVINHAKPDLIEVNLSCPNIPGKPQIAYDPVDSERLLKRVRPLITVPMGV
                     KLPPYFDPAHHAVMAEVIGRCGVDYLNLINSVGNGLVIDPKRETPVIKPKGGFGGLGG
                     SLIKPVALANVRAFWKLLSGRIPIIGTGGVVQGLDAFEHVLCGASAVQVGTVLVEEGV
                     GVFERLERELEAELSSRGARRVEDYRGRLKEL"
     gene            complement(19292..19966)
                     /locus_tag="PLS35_04625"
     CDS             complement(19292..19966)
                     /locus_tag="PLS35_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17973.1"
                     /translation="MSVHNLSKEYRRGGTVLCALQGVTLEVKAGEFCAFVGPSGCGKS
                     TLLNLIGGLDQPTSGTIMIEGRAVTDASATEWTRLRRELIGIVFQAFHLIPGLTVAEN
                     VALPMLLKGEQGRAVTDRIDEVLQAVGMEARRSHRPSELSGGEQQRVAIARAIVHRPR
                     LILADEPTGNLDSKQGADIVNLFRTLPQRFGHSVLLVTHSDSAAEAADYVWHMRDGRL
                     VNRIAH"
     gene            20198..20995
                     /locus_tag="PLS35_04630"
     CDS             20198..20995
                     /locus_tag="PLS35_04630"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNI17974.1"
                     /translation="MIMDFLRQASRLLLPMDCTTCAQPLADDPVPFFCRRCWELIRPL
                     RGPSCPRCHRPFASPSALTYSPTHECQDCRTREPYYSQVWAPYAYCSPLQDAIALFKY
                     RGKVALADALGVLLLQAIPDRLDVDVLMPIPLHPTRLRQREFNQSLLIADHIGSARRL
                     PVSYRNLIRTVDTAPQTSLPRAARLQNLRKAFAIRQPKEITGARILLVDDVFTTGTTA
                     SECARVLLTAGAKQVAVLALARSADAGMVPDTWLPPSTLNQTEFQRA"
     gene            20999..21343
                     /locus_tag="PLS35_04635"
     CDS             20999..21343
                     /locus_tag="PLS35_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNI17975.1"
                     /translation="MPIYEYRCRQCGKRTTRLILNANSVPQQTCAHCQSADMERLLSR
                     FASPKSEEARLEALSDPSNLSGLDENDPQSMARFMKKMGQEMGEDLGDDLDAAMEDGQ
                     APASGIDGTDFD"
     gene            21421..21861
                     /locus_tag="PLS35_04640"
     CDS             21421..21861
                     /locus_tag="PLS35_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17976.1"
                     /translation="MFAGEHLCKVDEKGRFLIPSPLRERFQAEGNQVMFLKNTEQTLW
                     MYSAKEWEQVLERTRATLDEDQTRLFMHHVMAQAGASDIDKAGRVLIPSRLRKLVPMD
                     EDQELVLVGMYHRLEIWGPSEWRRYLTRNEDRAEQDMAKIQNLL"
     gene            21954..22517
                     /locus_tag="PLS35_04645"
     CDS             21954..22517
                     /locus_tag="PLS35_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HNI17977.1"
                     /translation="MERAPLIPTQSNRNRRALSFPTPAGTGLSVTIDSITLTLPVPPS
                     INHQYATVNGRRLLSAKGRTYKDFVGQQIVVALAQSPHRATLRQTLQQASLSLSIHFF
                     FASALRRDTDGGLKIAQDALCEGLGLNDNRVVETHLYKHQDRDNPRMEIRLSVAPSTA
                     PDPFFPASLKAQVAVIPRPPIDNGQDR"
     gene            22573..23811
                     /locus_tag="PLS35_04650"
     CDS             22573..23811
                     /locus_tag="PLS35_04650"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNI17978.1"
                     /translation="MTYRPITIDALRIGMHVAKIDVAWFRSPFMRHSFLIRTDEQIEK
                     LRRAGVKQVSIDPSRGLDLQDPMAPSQAPLPVAAQPATSQMGKSVEIRSLATMTQELL
                     AARSARAKLEESVHSAFSRIAKTGVVDPEEASHAVHAISAVAQALNTSALFMVFSQGR
                     EANAPLSQHALATCSFSMILAHAADYNLMAIQELATGALLHDIGLLQVPPAILRRVHD
                     TSTTVSEQNRLTYEAHARSGAILLERRGGFTKPVEQIVAEHHAYLNGSGFPAETGGAF
                     TSDMTRIVMVTDRYDELMTGFGGASPLTPHQSLQRLYQEGQEGRYESRLISLFVKVMG
                     IYPVYSYVQLTTGERAIVSVINSGKLHQPIVTITHDPSGEPYIVPLVIDLANQDEEAP
                     PRGVRSVLGTIPEEFQRAYH"
     gene            complement(23884..25119)
                     /locus_tag="PLS35_04655"
     CDS             complement(23884..25119)
                     /locus_tag="PLS35_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI17979.1"
                     /translation="MPAFVLLTVMTALRILSRNRLRAGLTMLGIVIGVGAVIAMVSIG
                     QGARAAVQAQVASMGTNVIVIMPGSTTVSGVRGAQGGAVTLTINDAAELKKRSPLLSD
                     TGWAKRDVMQIVNGNRNWNGPVNGVSPSYLTIRDWSFTSGGPFTQTDMDSAARVALIG
                     QTTLENLFDPGEEAMGATIRIKNVPFQVVGVLAPKGQSAQGTDQDDVIFIPFTTAERR
                     VFGSQFLGSVGALFASTEQSGDLPEAVEHIRETLRLRHRLEPDQADDFTIRTQVDIGK
                     VQESTSQTLTVMLFAIASVSLLVGGIGIMNILLVSVTERTKEIGVRMAVGAKRIHILT
                     QFLIEAMTLSLFGGLIGVIVGIVGARLTTVIAGWPTMISVDAVLTAFLFSLAIGLFFG
                     LYPANKAARLNPIEALRYE"
     gene            complement(25126..25878)
                     /locus_tag="PLS35_04660"
     CDS             complement(25126..25878)
                     /locus_tag="PLS35_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI17980.1"
                     /translation="MVETVACDPVCEGTMAPLIVCEDLWKIYRIGDVEVQALRGINLT
                     IDRGEFVAVMGTSGSGKSTLMNLLGCLDKPSRGRYRLDELDVSTANPDLLADLRNRQI
                     GFVFQNFNLIPRTSALENAQLPLFYRGVSIKEQRRQAAAALQRVGLAGREQHYPAQLS
                     GGQQQRVAIARALVGAPSILFADEPTGNLDTASSREIMDILEHLNREDGITIILVTHE
                     PDIAAYASRELVMKDGQIVQDVRREPHLSAVT"
     gene            complement(25881..27164)
                     /locus_tag="PLS35_04665"
     CDS             complement(25881..27164)
                     /locus_tag="PLS35_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI17981.1"
                     /translation="MRRVSIIVGVLALGLAIAGYVFFNGERKVPLRFRTVPVERGSVV
                     SLVTATGTINPITTVQVGSQVSGMIESLHADFNSKVKANQVVARIDPFPYQARRDQAV
                     ASLANAKAAFEKARIDLAQRRRELDRAKSLIGQQFISQNEVDVALTASEGAVAQLKVT
                     EAAVKQAEAMLQAAELDLKYTVIRSPVDGVVISRLVEVGQRISASFSIPTLFLIAEDV
                     TKMQVDTNVSEADIGGIVDGKAASFTVDAYPGEAFQGRVRQVRNAPINIQNVVTYDVV
                     VEFENPEFRLKPGMTANVSIVVNKRENVLKVPNAALRFVPPKAVRDEKGEAGPRRDGE
                     RAGGRPTIASGSPESGNRQWGVWRLDGAGELERVPVEMGISDRSYVEITAAGIQEGDQ
                     VVTGIESPRGERKSGELPPGFGGGQQRGGRRDRGM"
     gene            complement(27353..28066)
                     /locus_tag="PLS35_04670"
     CDS             complement(27353..28066)
                     /locus_tag="PLS35_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNI17982.1"
                     /translation="MRHVWVAGLALMSAMAVSGCVSDKKYHEALADADATRGELERAR
                     AQKNALEQQVKTLKDLNAKFGSESQIARDELQRIQHGRDKERDTIDVRTKELEDKVKQ
                     LSAQNRSVKQEYEEARRHNEQLKSLVARYQKEMKDRSRSLSSGMESPALTAPLGGGGA
                     SSKAAPAPAPFEAPAAPLLNINKAPSSDLVLTLGITQDVADRIVSNRPYRVKGELVAK
                     NVVTKDVFDDIKDRITVSP"
BASE COUNT         6057 a         8946 c         7685 g         5474 t
ORIGIN      
        1 catgcagcag gttgtccagc gccgccatgt ggatgagcac gccaggcgtg gcggacgaaa
       61 acggcgtcac gcgcagatca tataaccctg ccgccgtccc ggcgacaaac acgatcttgt
      121 ctttgaacag ggacgcatcc aagtccggac ggtccccttt ttcttgctgc gcgaaggcct
      181 gcaagacacg gccgatcgag tatctcttgg cgtggtaggt ctgctccagg gacccatgcc
      241 atttgatcag cagattgccg tcagcatcca gaggaacgct gctgtcgccg atctgcaagc
      301 ggtcgtcccg catcgcgagc tcgctaccgc ctaacacgta tcgggctacc gccaacgaga
      361 gatgcggcac cagatttcct tgcacctgcc ccagcagggg aagacgcctt gtcggcccgt
      421 cggcgtcggc agacagattg atgacgccca acccacgcgc ctgttgcgcc aacacaggaa
      481 tgggcaattt cacacccggg tgcgctgcag cctgtaccgg tcctgagtcc gatcgttcgg
      541 cctttacggt ggcatggtcc tgcagcccgg ctggaaccgg cgccgcttcg gattgaaaca
      601 acatcggcag gaagacattc cccgcggcct tcacatcgtc ggcgaaggat tgatcgaatt
      661 cttcggcatt atcgtccggt tcgaaaaaca tcacatcgaa gacgaccgcc ttggctccag
      721 cccgtttgag ataccgcacg acatacccgt accggtcacg cggccagggc cagcgcccga
      781 acgcttccag gctggattca tcgatggcca gcagcacgag acttgaatcg gccttggcag
      841 gatcggcata gcgccggacg agatggtcga aggccttgag ttccaccacc tcgaaccatc
      901 gcgtcatgcg gagcccggtc accagggcat agaccgcaac tccgacggca atcgctgaaa
      961 ggattttttt ggttcgtgga gaaaatggca tgaacaagac aggtccgccc ccgatcatgt
     1021 cacaggcggg ctatcgtact ggagaacgag aaagagcgaa aggggctacg ggtgtttttg
     1081 agcgggtctc ggcgggatct caccgcagct gcacaatcgt gacgccgtcg cctccctctg
     1141 cgcgatcgcc ccctcgaaac gccaccacgt acggtgagtc tttcaggtag tctcgcaagg
     1201 cgcttctcaa ccggccggtc ccgtgcccat ggatgatgcg tacgaacggg ctaccgccga
     1261 gaatggcgcg atcgagaccg gcgaccacca catccaacgc atcgtcggct gcctgaccac
     1321 ggacatccag cgtctcctgg atgtcttcag gcgccaaggt ctggctggtt cgcctcggtg
     1381 agacaacgct cgttttgacc gcggctggcg cttgtggctg gcgagccttg gagactcccg
     1441 ccaggctcgc cacattggcc aggatttcgc cctcgccgac tttcagacgc acgcgttttt
     1501 tgccctgggg attttccagc aacgtacctg tcatccccag cccgatcact tcgaccgcat
     1561 ctccgatggt cagctgatca atcggaatcg gttcttcgga gtctccggcc tctcgccgga
     1621 ctgactcttc caattcgacc agccgggctt tggcctcctt ggccttgagc agcttttgat
     1681 cgcgcttgag atcgtccatc gtcgcctgca ccgcggcgcg ggcccgttga aactcctgag
     1741 acaacttttt cttcaacccc tgccgctcat cccgctcgga ttcctgcagg aacgtgagca
     1801 actcctgcgc ctcgcgagaa gcctgctcag caccctgcct ggccgccgtg gccgccacca
     1861 ggtcgtcatt gagtcgtcgt tgtttgtcct gcaaatcgct caacagtgtc tccagcaacc
     1921 ggttgtctcc ctgcagccgt gcgcgggcat cctccagcag cgactgatcc atcccgagcc
     1981 gcccggcaat ttcgattgcg gccgagccgc ctggttgccc ttgaatgaga cggtaggtcg
     2041 gcgaaagcgt gtcgagatta aattccacac tggcattggc gaagcccggc ctgtcctggg
     2101 ccagaccttt caggagactg tagtgtgtgg tggccaccac tttcacccca gccgcagcaa
     2161 gccgacagag cagtgcactc gcgagcgctg cgccttcggc cggatcggtg gaggtgaccg
     2221 gctcatctaa caacaccagc gctcgtccgg gaggcaccgg ctccctgtcg tgttcactcc
     2281 ccgctccatc agacacttcg ttcagtaact gcaccatttg tgtgatgtgg gcagaaaaac
     2341 tggacaggtc acgcgccaga tcctgcgcat ccccgatgtc tgcatacacg gacggaaaga
     2401 tcgccatctc cgactcaggg gcacagggaa gatgcagccc gcaacggacc atcagagaga
     2461 acagacccag taatttcaag atgaccgtct tcccgcccgt gttcgggccg gaaatgatca
     2521 gtacccgtac ggtttcatcg aacgcgagat cattcgacac gacctgatct ttgctcacca
     2581 ccagaaacgg gtgccgcgcc tgcgtaagga gaatgcgtcc ctggtcattg agccgcggcg
     2641 gggaaccttg cagacgttga ctcaggcctg cctttgccga aatcgcatcc agccgaccga
     2701 gcagagtcac actgccggcg agcgccggtt gatgaggggc caccagcgcc gagagttccc
     2761 gaagaatcct gcgcacctcc cgttcgatat ccagatcgac gaccttgatg gagttattga
     2821 gatccaccag ttcccgaggc tccagaaaca gtgtggcacc gctggatgag acgtcgtgca
     2881 cgataccggg catgcggcct tgcatctcgg ccttgatcgg caccacataa cgcccttccc
     2941 gctgtgcaaa atatcgttct tgcaacactt cttcatagcg ccgcgaatgc agaatttgct
     3001 cgagccgatg tcgcatctgt tgtttgaaat cgttggcccg ttggatcaac cggtgtaact
     3061 cagggctggc tgattcacga atggacccat cggggtcgat ggccccgttt agcgcgaccg
     3121 ttagccggcg gtattccgtc accggtccca actgctcggc catggcgccg accgtcggag
     3181 cgtcgggctg atgacgcagc aggtatcgtt ggacatccac gaccaattcc aacaccagcg
     3241 cgatgtctcg tagctcatgc gcctccaaac aggcgccttt caccactcgc ctcaaccact
     3301 ccgtcacgtc agggaatcgc agcacaggaa aagggtcgac gccggcccgc aggcgcaaca
     3361 tgtcggaggt ttcttcctgg cgaagccgag cctgcgccac gtcccgctcc aattccatgg
     3421 accggcattg ctccgcccca acctccgact gggcgtagcc ggccaacaga tccaacagtt
     3481 tggcccactc caagaccttg cacgcttcga actgcagctt ttcagattgc atcacgcccc
     3541 atctcgatga acgctcaggc atgagactct accggacccc atacgtccac aacaagacgg
     3601 ccaacacccc atcatttccc tgaggccctt tcgccaatat ccaccggcct tccctctttt
     3661 tgtatgcttg acacgcagaa agtcggtcag tactatcgcc ccagacttac tctgttcaac
     3721 ccgtttaatt cttgaggatt tagccccatg accacacgca tcggcatcaa tggattcgga
     3781 cgcatcggcc gcaacgttct tcgcgcttcc ctgggagatc ccagcctcga atttgtcgcc
     3841 atcaacgatc tcaccgatgc caaaacattg gcctatttgc tcaagtatga ctcggttcat
     3901 ggcacgctgg acgcttccgt cgaggccaaa gacgaccagc tcatcattga cggccgcgcg
     3961 atcaaggttc tcgcggtccg ggaccccaaa gagctcccat ggaaagctct cggcgtggat
     4021 atcgtcgtcg aatcgaccgg ccattttacc gaccgcgaag gcgcgggaaa gcacctgtcg
     4081 gccggagcta aaacggtcat catttccgcc cccgcgaaag atcctgatgc cacggtggtc
     4141 cttggagtaa atgagcaggt cttcgacgcc aaagctcacc atatcgtctc caatgcctcc
     4201 tgcaccacga actgtctggc cccggtggca aaagtcttgt tggaaaactt cggcattaaa
     4261 cacggcgtca tgacgacgat ccactcttac acgaacgacc agcagctcct cgatctgccc
     4321 cacaaagatt tgcggcgggc gcgtgctgca gggatgtcga tgattcccac cagcaccgga
     4381 gcggcgaagg ccctgcacct ggttattccg caactgaaag gaaagctcga cggattggcc
     4441 attcgcgttc ccacccccaa tgtctcgctg gtggacctca ccgtcgaaac ggagaaggat
     4501 tgcgatgtgg ccggcgtaaa tgccgcattc aagaaggctg ctgaagggcc catgaaaaat
     4561 gtgctggcct actcggaagc acccatcgtc tccatcgacc tcaaggatga tgcccactct
     4621 gccattgtcg acgctccgct gaccgccgtc atcgataaac ggctcgtcaa agtcaccgct
     4681 tggtacgata acgagtgggg ctattcctgc cgcgtgcggg acctgatcaa gtatattgtt
     4741 gcaaaatcgg cgtaacaggc gtcacgcacg acacgctttt ctgacgtgca gcaccggccg
     4801 gagctgccag cgcccggcgc ggacacacca gaggaggagt gaggatctgc catgaatatt
     4861 cgcaaacgga ttatcgagga cctgcaactt cgcggaaagc gcgtcattat tcgcgccgac
     4921 tacaacgtac cccttgatga ttccttgcag atcaccgacg acacgaggat tcgatcgacc
     4981 cttcccacca tcaatcgtgc cgtcgacgag ggcgccaagg tcattttgtg ctcccacctc
     5041 ggccgtccga aaggcaaatt cgatccgaag ttcagcctgg ctccagtggc caaacgcctc
     5101 cagcgcctgc tcggcaaaga agtgattttt gcgccggatt gcatcggctc cgccgttgaa
     5161 ggcctggttg ccaagatgca gcctggcgat gtgatgttat tggaaaacct ccgctttcat
     5221 cccgaagaag aaaagaacga cgaaggattc tccaaggcgc tggccgcact cgctgatgtg
     5281 tatatcaatg acgcgttcgg tgccgcccat cgagcccatg catccacggt cggcatcacc
     5341 aaatatattc ccgaagcggc agccgggtat ctccttaaga aagaaatcga atacctggag
     5401 ggagccgtcg aaaatccggt gcgtccgttc gtagccatct taggcggcgc caaggtatcc
     5461 ggaaaaatcg gcgtgatcga gaatctcggc aagaaagtcg acaaggtcat catcggcgga
     5521 ggcatggcgt tcacgttcct caaagccatg ggtcttgaga tcggccagtc actcgttgaa
     5581 aacgacatgc tcgactttgc caaaggggtg caggaccacg cctttgccag tggcgtcaaa
     5641 ttctatcttc ccgtggactg cgtcgttgcg gccggccggg agcccggagc cgaaacgaaa
     5701 atcgttccgg ttcaggaaat tcccaaggga tggtatggcc tggacatcgg tcccgcctcc
     5761 gtaaaattgt tctcggaagc cgtgcaggat gcgaaaacca ttctgtggaa tggccccatg
     5821 ggcatgttcg aagtcgacgc cttcgcgaga ggcaccctcg ccatggctca ctccgtggcc
     5881 aacgcctacg cgctgaccat tgtcggagga ggcgaaacag ccctggctat tcaccgcgcc
     5941 ggagagtccg aaagcatttc gtttatctcg accggtggtg gcgcagccct ggagctgctc
     6001 gaagggaaaa ccttaccggg acttgccgcc ctccccaatc gtgcagcgta acgacaccct
     6061 ttctccattc tcccactaga ggcttcgtga gaacccgctt cctcatcggt aactggaaaa
     6121 tgaataagac cgccaccgaa gcgggggcgt tcgttcgtcg cctcacccag gagttgccta
     6181 ggtctgaagg aatccaggtc ggttttgcac cgccctttac cgcgctgcat gcagcccatg
     6241 aggcgctcgg ttcctcaccg gattttctgc ttggcgcaca ggacgtgtat tgggaagata
     6301 agggggcgtt caccggagaa gtgtcgggaa ccatgctgaa ggatctcggc tgtcaatttg
     6361 tcctggtggg ccattcggaa cgccgccagt acttcggcga gcaagacagc tggaccaaca
     6421 agaaggttct ggctgctttg cgtaacggca tccaaccggt tctctgtgtg ggcgaaacac
     6481 ttcacgatcg cgataccaat caaacagacc aagtaatcca acgacaggtt cgcgcaggac
     6541 tggctggaat cgaggggcaa catcttgagt cgatggtcat cgcctatgaa ccagtgtggg
     6601 ccattggaac ggggcgagcc gcatcgccgg aacaggcggt gccggtccat cgattgatcc
     6661 gccaaaccat caatgaactg agcggagctg aagcgggggc gcgtgttcgg attctgtatg
     6721 gaggcagcgt cactccgcac aatatcgccg aatttctggc ttccgaagaa atcgacggcg
     6781 cattagtcgg cggggcttgt ttagatccgg tctcttttgc tacactcgcc aaagtcgcga
     6841 tcgggtccaa acctaccacg gcctaaagac tcaaccatgt acacattgct catcatcatt
     6901 catgtcatcg tctgcctcct tatgattgga gccattctgc tgcaatccgg taagggcgct
     6961 gaaatcggag cggcctttgg tggatccagc caaaccgtgt tcggcagccg agggcccgcc
     7021 aactttttga gcaagttcac ggtcatcacc gcgtttgtct tcatgttcac gtcattgtct
     7081 ctggccattc ttgcgaagga tcgaaacttt tcctcgacgg tcattgatct gaacaagaaa
     7141 cctgcggcaa ccactcccag tacgacaccg gcagaaaccg cgacgccgcc ggcgaaagac
     7201 tcgcattcct ccaccgatag ctcccactaa gacctactgt tccagctagc cttcgattcc
     7261 acgcaggttt cgatcaacga gccgaatcgt ttcctctcgc tctgactaaa aagtgagacc
     7321 cctatgattg ctcgcgcggg cactacgcta gtagtggaat ggagaagcac tctgagtgca
     7381 ggagttcgaa ggccagtgca gcgttcgcgc cacagcacat caatgtgatg aatgctcgac
     7441 taagagatca ggtgaggtta ccgagacttg ctaaacggga gtcagccact gactgtgagc
     7501 cgcatcggtc ccgcgcacga catcaaaaaa cgctttctgt aaggtctgcg tgatcgtccc
     7561 tggttttccg gtcccgatac gccgctgatc aatttcggtc accggcgtga gttctgcagc
     7621 cgtccccgtc acaaaaactt cctcggccac atacatttca tcacgggtga atcgttcctc
     7681 aaccacggga atgttgcgct ctttcgccag ctgaatgatg gaattgcgcg taatcccctc
     7741 cagaatcgag gttaacggag tcgtcttcaa cacaccgcgt cgcacgatga agacattttc
     7801 tcccgtcccc tcagccacat aaccttcggg atcgagcata atcgcttcat catacccgtc
     7861 ggctttgacc tcccgcttgg cgagaatcga gttcacgtag tagccggaaa ttttgccacg
     7921 cgtcatggac acgttcacat ggtgtcgtgt aaatgaagac actctggcac gaatgccctt
     7981 ctgcagcgcc tcatccccca gataagtccc ccatttccag gcagcgatac tgaccttgat
     8041 gggattttct cccggataca gtcccatggc gccatagccg atatacacga gcgggcgaat
     8101 gtagcaagcc tctagccgat tgactctgac cgtttcgata atggcgtcgg tcatttgctt
     8161 tcggtcatag ggaatctcga tcagaccgat gtgcgccgac tcaaataggc ggtccacatg
     8221 ctcgggtagc ctgaaaatgg ccgacccgct ggtgccttta tagcagcgga tgccttcaaa
     8281 ggccgctaat ccataatgaa gtgagtgggt caacacatga acctgtgcat cggcccacgc
     8341 cacaaacttg ccatccatcc atattttttc gctggtttgt aacatgcgcg ggataatacc
     8401 agcggctcgc gatgagcgtc aagataacag ccgccacgct cccgggccgt agttttgttg
     8461 cctccgaccg tacgactcca cttcttcgtg ccgcacgaca aggaatggtt tcgcgcaaca
     8521 ccaggactaa ttcttgacac tctcgccgag gctgtgataa acacccacgt tctgctgacg
     8581 aggagaagga cgcacatgta cgctattatc gaaacaggtg gaaaacagta tcgagtggaa
     8641 acagggaccg tactccaagt ggagtcgctc ccgggcgatg tcggccattc cgtgcagatc
     8701 gacaaggttc gattgctgaa tggcgacagt ggactggtca tcggccagcc ggtcgttgcc
     8761 ggcgcgcgag tcaccgcgga aatcatccgc cagggacgca ccaggtccat cacggttttc
     8821 aagaagcaac gtcgcaaaaa ttatcgccga accagaggcc accggcaggg ctttacgcag
     8881 ctgcgggtca ctgagatcgc atcgaagtaa cgactcgaag gggagtttgc catggcaaca
     8941 aataaaggcg gcggttcgac aagaaacggc cgtgacagta acccacaata cctcggcgta
     9001 aaagcctacg gcggagagac catcaaggcc ggctccatca ttgtgcgcca acggggaacg
     9061 aaattcttcc ccggcctcaa cgtcggcctt ggacgcgatc acaccctcta tgcctacgtg
     9121 tccggtgtcg taaagttcga aggtggccgc ggacggcaaa aggtcagcgt ctatccagtt
     9181 accgcgaagg cctgatccct ccttagcttc agtcttgttc cgcggaacag cgccccttcg
     9241 cttacgcagc atcccccatg agccggcgga tacgctatac tgtgattcct gaataaatga
     9301 cgccgggtgg gggctcgctg cgatgtctac atttgtcgat caagtccaaa tcctggtaaa
     9361 ggccgggcac ggcggcaacg gagcctgcag cttccgtcgg gaaaagtttg tcccgcgcgg
     9421 cggccccgac ggcggggacg gcggggacgg aggcagcgtc attgtcgagg ccacgactcg
     9481 cctcagcacc ctgctggatc ttcgatacca aaaacattac gaagcagaaa acggcgaggt
     9541 cggcggcggc agcaactgcc agggtcgacg cggcgcagac gtgcggatac ctgtcccggt
     9601 cggcacgatc gtgctcgatg caaatacgca ggaacttttg gcggacctga ccgcggatgg
     9661 cgagagttgc gtcatcgcca agggaggact gggaggccgc ggcaactcgc gattcgccac
     9721 ctcaaccaac cgcgttccga ctcagttcga gccgggcaca ccgggcgaag aacgcctcct
     9781 gcgtctcgac ttgaaactcc tcgcggatgt cggattagtc ggctacccga acgccggaaa
     9841 atcgacgctc atcgccgccg tctctgcggc gcgtcccaag atcgctgact atccctttac
     9901 aacccttaca ccgaacctgg gtgtcgtccg ctcgacgggc gaacacagct tcgtcattgc
     9961 cgacattccc ggactgattg aaggcgccca tgaagggaaa ggcctcggct ttcagttcct
    10021 ccggcacatc gagcgcacca gcctgctgat ccatgtcatc gatatttcag agtgggccac
    10081 ggaggagcca gtggcgagcc tcaaggtcat gcgtcatgaa ctctccgcct acgatgaggc
    10141 actcgccgcg cgtccctttg ccgtagtagg cacgaagctc gacatcaaag gcgaaggcga
    10201 gcgcctcgaa caacttcgga aatattgcca gcggcgcaag attccatttt ttgcgatttc
    10261 ggcagccacg cgagaaggtc tggacgagtg catactctac atgggccaac aggtggaact
    10321 gctccggaag accccgtgcg agacgaactc ctaaagcaag ccagacgtgt cgttatcaag
    10381 atcgggagca gtctggtcgc ctcacgcgaa tccggcctga gcaccgagcg catcgaacga
    10441 ctcgcctccg aaattgccca cgtgcgcgca caggggcgag aagtgctggt cgtctcgtcc
    10501 ggtgccgtcg tctccggcat caaaaaactg gagctacgcg agtaccccaa aagcctgccg
    10561 gtcaaacagg cggccgcggc ggtcggtcag agcctgctga tgtgggccta cgagaaggcg
    10621 ttcgagcgcc tctcgcttcg cgtggcccaa gtgctgctga cccatgaaga cctggcggat
    10681 cgccgtcggt ttttgaacgc ccggcacacc ctcaccacgc tgatcaaatt cggcgtcatc
    10741 cccatcatca atgagaacga tacggttgcg gtcgaagaga tccgcgtagg agacaacgat
    10801 acgctggcag cacaggtcgc ccacctcgta gacgccgacc tgctgatcat tctgtcagac
    10861 gtggatggac tgttcaccgc cgaccctcgc aaagatttac aagccacctt gattcccctg
    10921 attcaggaga tcacggccga cattgaacaa cgggcgggca tgtcctccac cttcgaagga
    10981 accggcggca tggccacgaa ggtccgcgcc gctaagaaag tgggcgaata cggggtcgcg
    11041 accttgatcc tgaacgggac tcgcacgggt ctgcttccgg atgtgctgtc cgggcaaccg
    11101 gggggcagcc tgtttctcgg ccgggagcga cgcatgaaca gccgcaaaca ttggattgca
    11161 ttcacgttgc ggccccgcgg acatgtgcaa ttggatcaag gcgcggtgga tgcactggtg
    11221 cgaaacggca aaagccttct ggcctcaggc attcgcgaca tcaccggcca gttcgaagct
    11281 ggggatccgg tcacctgcgc cggacctgac ggcaaagagt gcgccaaagg cctggttaat
    11341 ttttcctcca cgatcctgac ccgcatcaag ggcctcaaga ccgccgatat ccaaaagatc
    11401 cccggtctcc aagagtatga agaggtcatt caccgggaca acctggtgct cttgtagcgg
    11461 ccgccagacg ccgccgatgc agggcgtcgt tcgacggggg cctgtccatc cgagacctgc
    11521 acaatcgtgc gtgaaggtag cctcctatgc gtctgggagt tttgggcggc agttttaatc
    11581 cgattcaccg ctgccatctc tcgattgcgc agtcggcgcg gcgtctgctg tgtctggatc
    11641 aagtcttgtt catcccgacc ggagatcctc cgcacaaaca accagacacc ttggcgtccg
    11701 cacagcatcg ctaccgcatg gtgcaattgg ctattcagga cgagcctgcc ttcaccctta
    11761 ccgatatcga aatctgccga tccggcaaat cctattccat cgacacggtc cgtgcccttc
    11821 aagagcaata cgggtccgac acggagattt tcttcatcat tggcctcgat gcctttctgg
    11881 acctgccttc ctggaaggat gccgcacagc ttcttagcct gtgtcatttc gtcgtaattt
    11941 ccaggcccgc cacaacgttt ctgaccgtgg cctcgctgcc tctctttccc gaagtgccca
    12001 aggaggagtt gctcgctctg gatacagggc ggctggagcg agcggacgtc ccattgaaaa
    12061 atgggcggac cgtgacattt ctaaggctgc cgccctgcga gagttctgcg tcggacatca
    12121 gagcacgtgt cgcaaatggc cgtcctctgg caaatctgtt gccccccctg gtcgaatcct
    12181 atatacttcg cgaggggtta tacagggagg acagtgagcg tttctgaaac aaaagccaaa
    12241 gctctcgcgg tggcggccgc cattctggac aagaaagcca ccgatgtcct gatcctgcat
    12301 atcgccaagc tgacctctat cgccgactat ttagtcatcg gctccggaga gtcggagcgt
    12361 caagcacggg cgatcgcgga ccatgtcggc gatgtgctca aggcccaggg gcatccgccg
    12421 ctcggtatcg agggcgcgtc gtctgccaag tgggtcgtca tggactttgg tgacgtcatc
    12481 gtccacatct tccaaaagga tattcgcgaa cactacgccc tcgaacgact gtggggcgac
    12541 gcaggacagg tcaagctgcc ggaagagcgc gccgtgaagg tgactccccc cgcacgaacg
    12601 aaggcccgtc ccgctcgcac ccggaaacgg gtctagcatg tttcggctcc tctccacgat
    12661 cttcctggtc agtgtcggca tctttctcta cagctatttc cgcgaactga acccgggcac
    12721 gatcaccgtc cggaccagtc ccgacgcgat cttcgagctc agccccgtct ccttggtgct
    12781 attctcgatg gccctgggcg ccacgctggt tgcactgatc gtgacgatca aagaaacctc
    12841 ccacgtcttc atgaattggc gcaccaatcg cctcgtccgc cgtaaagaga aagtggacgc
    12901 gctccaccgg gatggcacgc acgccttcat gtccaagcgt acagccgatg cggtgagtct
    12961 gtttgagcgg gccctggtca ttgatccgaa ccggaccgat tcgctgctct ggctcggaaa
    13021 tatctatcgc tctgaaagca actttacgga ggccatccgg ctccaccagc aggcctatcg
    13081 cattgaagaa cgaaatgtgg aggtgctgct cgaattagcc aaagatcttg aaggcgcccg
    13141 tcgctatgaa gaggcacttc agacactcca gaatattctg cggatcgagc cggacaatct
    13201 aatggccctg atccgcaagc gcgatctgca aatcaggctc gaaaaatgga gcgatgcgct
    13261 ggagattcaa caccggttgg tcaaggctaa cctgccggaa agcgagcgac gcgccgaggc
    13321 caatctgtta ttgggctgta tgtacgaagt cggccgtcaa ctcttggaac gagggcaccc
    13381 ggacaaggcc cgccggtact tccgaggcgc gattaagaaa gatcgcacgt ttctgccggc
    13441 ctacatcggc atcggggaaa tcttggtccg tgagggcaaa atcaagaacg cggtcgaaat
    13501 tctcaagaag atttacgcca agacgcgcag cgtcatcatc cttcaccgcc tcgaggaact
    13561 gttcctggag ctgggagaac cggatgaaat catccgtgtc taccaggaag ctctgcaaca
    13621 ggatccgcac aatgctgtga tccagttcta cctgggcaag ctgtattacc ggctcgaaat
    13681 ggtcgatgaa gcgtacgacg tgctttcaac gctggatggc acgcaggaac agctcgttga
    13741 ttaccataag atcatggcca atctgttcct gcgcaagcaa cacatggacg aagcggtggg
    13801 cgagctcaag aaagccctgg gcttcaaaaa gcgagtcgtg gtgccctacc aatgcaccca
    13861 gtgccaccat gagttggccg aatggtccgg ccggtgccgg cggtgcggcc gctggaatac
    13921 ctacgtcgcc cttccctgga gagacagcac caaatccggt gccgatcagg acggacaggc
    13981 tgcccggctg cctgccgtcc cctatcaagg cattgcatct ccgtttgaaa ccgtgtaggg
    14041 tttccaccgc ccttttttca tgttacagac agatcctttg ctcacggagg cattgctgaa
    14101 ggagcggggc ttgcctgttt tttcgaggag tagcgtgcat gactgatttc acgatcctgg
    14161 ccgacaaggc cttgcgggac gaattactca gccgagagga atgcctcacc gtactacata
    14221 caccggacac caacctgctc gagcttctgc aggccgcctt taaggtccgt gaacggcact
    14281 tcggtaagac ggtgcgcctg caaatgctct tgaacgccaa gagcggcgcc tgccaggaag
    14341 actgcggcta ctgctcgcaa tcatccgtgt ccacggcgcc catcgaacga tatggattgt
    14401 tgccgcaaga tcaaatggtg accggcgcgc gccgtgcggc agccgcgaaa gcccaacgct
    14461 actgcatcgt catcagcgga cgcagcccgc tggatcggga gatcgccgat atcgcctcag
    14521 ccgtccgatc catcaagcag gaagtcccga tccagatctg ctgttccctg ggactcctca
    14581 acgaacggca ggcaaaagac ctcaaagcag ccggcgtgga tcgcatcaat cacaatttga
    14641 atacgagcga agcctatcac acggaaatct gcagcacgca cacctttcaa gaccggctcg
    14701 ccaccattcg acatgccagg acggccggac tggaaatctg ttcaggcggc atcgtcggga
    14761 tgggagaagg cgatgaggac ttgatcgatt tggcactggc cctgcgcgag gtcaagcccg
    14821 attcgattcc gctcaacatg ctgcatccgg cctccggcac gccactggag cattgcactc
    14881 cgctgacgcc gcagcggtgc ctgaaagccc tctgcctctt ccgcttcctt cacccgcgga
    14941 cggagcttcg tatcgccggc ggacgagaac acaacttacg cagcctccag ccattggcac
    15001 tctacccggc cgattcagtg tttgtgaacg ggtatctcac cactccggga caaccggccg
    15061 cagaagtctg gcgcatgatc gaagacctgg gattcgacat tcaagtggac gcgcttccga
    15121 cacagggtgc cacttcgtcc gccgaaccgg ctgtcggcct tcattcataa cgcagtccct
    15181 cgaccaaggg ttgtctggcg gcccagcggg ccggccagaa ccctgcgacg agcgaggcca
    15241 ccagggccaa ggccgcaacc tccgccatca ggccgactgg ccacgaaatc tggatggtcc
    15301 aaccgaatga ctgccggttg atcacccgga tcaggatgag cgccagggct ccccctccga
    15361 ccaaacccat ccctgtcccc agcaatccca aatagccggc ctcccataaa atcaacgccg
    15421 tgacctgccc ccggctactg cccaaggcct ggagcgtggc caattcacga cgccgctcaa
    15481 ccaccgacgt gaccaacgtg ttgatgatac ctaacgtcgc aataatcacg gcgatggctt
    15541 ccagcacata ggtcaacgta aacgtccggt cgaaaattcg aagaatctcc tggcgcaatt
    15601 ctgcattact caatacggtc ggcatgagat ttccctgcga cgcctgagcg agggtgccga
    15661 gaatcgccat tcttgccttc cccatatcgg cccccgattc gacatagacg ggaaacacgg
    15721 tcacgccgtc atcaggccac cattgttgat acaaggatcg atcaatgacg atctttccgc
    15781 catcggtggc ataatcataa aagactccca gcacctccag ggactgttcg ccctttgggg
    15841 tcacgatgga caatcgatcc cctttgccca gcgccaaatg attcgccacg acttccgaaa
    15901 gaatcgcgcc ttccccggcc gcggcacgag ccaaaattgg ggcggactcc cccgtgacga
    15961 aaagataccg gctccgtgcc gcatggagtg ccaggtctct cgaaaccagc gagaccggcc
    16021 ggccctgcag atcgatacgc aggtcacggt acatgtctac cgccgagacc agaggagcat
    16081 tggccaaccg agcccgccag gcaccaggca gtaccggact ggacgacccg ctgcgcacgg
    16141 cgtgcggcca tccggcagga gcgacaataa agtccgccat gacggtctga tcgatccaga
    16201 tttcaaccgt gtcgcgaaag ctgcggatca tcaccagcac gccgaccatg atcgcgacgc
    16261 ccacgagaaa ggccgacacc gtgacggcat ttcttcccat gccccggctg gtctgctctc
    16321 gggcaatgtg ccgaatggcc ccgcccaacg tcggcgcctg cgaaagcgtc gttaactccc
    16381 gggtacggca gaggctctgc aacaggatgg ggacgaggca ggacaggccg gcaagaagac
    16441 aaaaggttgc cacgtacccg aagatcggta cgccacccac cgggccggcc aatgcgcatc
    16501 ccagagcgat gagtaacagc actccacccg ccacccccag tgacgccgcg cgaacacgtt
    16561 gagcgacgtc gtactcaccc ggtgccagag ccgcgaccac aatcgtacgt ccggcatcga
    16621 cgctcggacc aagcgcgccc agaactgaca ctccgctccc gatcaatacc gcttcgacca
    16681 ataaccggcc agacccgaca ggaaaaccaa cagttcctgt cgaatgggac agcggtgtat
    16741 acagatcatg gatcgtccgg ccgaccagcg cgacaagcac atcgcccaac aacaggccga
    16801 gcatgcctcc gagcagcccg cccacgacac cgaacactcc ggcttccgcc agaaacacag
    16861 cgacgaccat cggctccgac atgccgatgg cgcggagaat ccccacctcc cgacgtcgct
    16921 gcgccaccgt aaaggagacc gtgttgtaga tcaggaagat cccgaccaac agccccacca
    16981 tgctcaacac ggagagattc agttgaaagg cccccaccat cgattcaacc tgctggctgc
    17041 gctgaatcgg gcgacgtacg gtggtagccg ggggcaaaac ccgcccgatg gcctccgcca
    17101 cctgctcaac cgacgaagtg ggcgtcgtca ccacatcgat acgatcaagg cggccgatca
    17161 gtccgaacgt ccgctgcact gccgcaatat ccatcaccgc tatgcggtcc cagggcgaag
    17221 gcccaccagg acgccggccc atgactccga taatcgacac cgacacgtgc tgatccccgc
    17281 tctgcagcgc caactgagcc cgtagcgcga gactcaactc cgacgctagg gcgtgcccga
    17341 tcagaaggcc atttcttgcc aggaggtctt ccagtggcat tcgctcacca gccccctccc
    17401 ctcgattcgc tggagaaacg acacgtcctc cctccgggct caactcttcc aataggtcca
    17461 ggccaaggat cgaaaacgac tgccgggggg accggccgtc tgtgacccgc accccgacct
    17521 ccagcaccgg tcgagccgac aagacaccgg ggaccgtccg tacgctggag atcagacgtt
    17581 cgtccaagcc ggtttccccg gccgacacct ccagcgtgac cgggccggct accgtcaaga
    17641 ctgattcctc gaaggaacgc aggacttcca cgttggccgt ttggacggca atagtggccg
    17701 ccacgccaag cccgacaccg aacatggtca acgctgtccg ccccggatgg gtggacaaat
    17761 gtgtccaccc caggctcgca cagactctca gtaaagaaga gtgaaacaac gacatacgta
    17821 cgcgcgttcg tcctacagtc tagtaattgt ttttacagat ttacccaggt atgttaagat
    17881 tactttcagt agaaattggg gtacaaaatc tttatggcac tacgcaaacg acctcaagca
    17941 aaaaaatcct cgaagaccac caaagctgct cccgcagcga agaaacgaaa gcccgtgaag
    18001 ttggccaagg cgtctcccgg acgcacaagg cgctcagacg gactcactcc ccgacaaaag
    18061 gaaattctta aactggtgtc gcaaggaaac accaaccggg acatcgcccg ccgtctggat
    18121 atcagcgtgc gaacggttga ggtgcatcgg tttaacttga tgcggcgatt gaaggtccgc
    18181 aacgtggccc aactgctccg ccaggcgctg cagcaaggtt tcctgccgaa gaactttgcc
    18241 tttagataat ccgaccgaag accgcgtggc gtggaatctc cgcgccacgc gttataactc
    18301 ttttaacctg ccccgatagt cctccacccg tcgcgctccg cgagaagaca gttcggcttc
    18361 cagttcacgc tccaggcgtt cgaaaacacc gacaccttct tccaccaaca ccgtgcccac
    18421 ctgaacggct gacgccccgc agaggacatg ctcgaacgca tcgagcccct gcacgactcc
    18481 gccggtcccg atgatcggaa tgcgcccact gagcagcttc cagaatgctc gcacattggc
    18541 caaggcaaca ggcttgatca acgaacctcc caatccgccg aaccccccct tgggtttgat
    18601 gacgggcgtt tcacgcttcg ggtcgatcac gaggccgttg ccgacggaat tgatcaaatt
    18661 caaatagtcc actccgcaac ggccgatcac ctccgccatc accgcatggt gagccggatc
    18721 aaaatagggc ggcagcttga cgcccatcgg caccgtgatg agcggccgca cccgcttcag
    18781 aagccgttcg gaatcgaccg ggtcataggc gatctgtggc ttgccgggga tgttcggaca
    18841 agacaggttc acctcgatga gatcgggttt tgcgtgattg atcacgcgcg ccatggtgag
    18901 aaagtcgtct tcgcacagac cggcaatact ggcaatcaca ggcttgccga actgcttcag
    18961 ctccggaatg aggtccgcat aggccagata gccgagattc ggaagtccca tggaattaat
    19021 cgacccgccg ggaaatccgt aataacgcgg ttcaggattt ccgccccgcg gttccaccgt
    19081 catcgacttg gtcacgatgg cgccggcccg cgagcgaccc aaagccagca attcctcccg
    19141 cgtcatacag agcgccccgg acgcattcat caaacagctc ggaaacgtga cgcccgcaat
    19201 ggtcatgctg agactcatac cgatcctttc tcttctgcat cacgaacagg gccagcgtta
    19261 ggaacagtaa gcatccgtca ccctcccgga atcagtgtgc aatgcgattg accaaacgcc
    19321 catctcgcat gtgccagaca taatctgcag cttctgctgc gctgtcgctg tgggtcacca
    19381 acaacacgga atggccgaac cgttgaggaa gggtccggaa gaggttcacg atatcggcgc
    19441 cttgcttgga gtccaaattt cccgtcggct catccgccaa aatcagacgg ggccgatgca
    19501 cgatcgcgcg ggcgatggcc acccgctgct gttcgccacc ggacaattca ctgggtcgat
    19561 gagaacggcg tgcctccatc cccaccgcct gcaggacttc atcgatccga tccgtgacgg
    19621 cgcggccttg ctctcctttg agtaacatgg gcaacgccac attctccgcg accgtaaggc
    19681 cgggaatcaa atgaaaggct tgaaacacaa tgccaatcaa ctcgcgccgg agacgggtcc
    19741 attccgtcgc gctcgcatcg gtgacggccc gaccttcgat catgatggtt cccgaggtgg
    19801 gttgatccag gcctccgatc aagttgagta acgtgctttt cccgcagccg ctgggcccca
    19861 caaacgcgca gaactctccg gcctttacct caagcgtgac gccctgcaga gcgcacaaga
    19921 ccgttccgcc acggcgatat tctttcgaga ggttgtgtac gctcacgagt ggggccgcag
    19981 aaccgctcat atcagcagga ctggacggtg aatgatcgtg ggatgttcca agcatcgtca
    20041 gccggccaac cctgtccaat ggtgaaatcg cccgcatgca gaccgaatgg tgctatatca
    20101 cacccccttt acagcccaca accttgacag gtctggagcg ttccttagac aatcccacca
    20161 ggctgaaggg cttcacgtca acgatacgta gtcgacaatg ataatggact ttctcaggca
    20221 ggcctcgcgg ctgctgcttc cgatggattg caccacctgt gcgcaacccc tcgctgatga
    20281 tccggtgccc tttttctgcc gccggtgctg ggagctgatt cgaccattgc gcgggccttc
    20341 ctgtccacgg tgtcatcgcc cgttcgcttc accctcggcc ctcacctaca gcccgacgca
    20401 cgaatgccag gactgtcgta cccgcgaacc ctattactcc caggtctggg ctccctatgc
    20461 ctactgttct ccgctccagg atgccattgc cctcttcaaa taccgaggga aggtggcgct
    20521 ggcggacgca ttaggcgtgt tactcctcca ggcgattccc gatcgcctgg atgtcgatgt
    20581 cctgatgccg atccctctgc accccactcg actccggcag cgggagttca atcagtcgct
    20641 tctcatcgcc gaccacatcg gttccgcccg cagactaccg gtatcctatc ggaacctgat
    20701 tcgtaccgtc gataccgccc cgcagacctc gctgccgcgc gcggcgcgtc tacagaacct
    20761 ccgcaaggcg ttcgccatac gccagccgaa ggagatcacc ggagcacgca ttcttctggt
    20821 ggacgacgtg ttcaccaccg gcacgaccgc gagcgaatgc gccagagtcc tccttacggc
    20881 cggagccaaa caggtcgccg tcctcgccct ggcgcggtcc gcggatgcgg ggatggtgcc
    20941 ggacacgtgg ctgccaccat caactctcaa ccaaacggaa ttccagcgag cctaacccat
    21001 gccgatttat gagtatcgtt gccgacagtg cggaaaacgg accacacgcc tgattctcaa
    21061 cgcgaactcc gttccgcagc agacttgcgc ccattgtcag agcgccgaca tggaacggct
    21121 gctgtcgcga ttcgcctcgc ccaaatcgga ggaagcacgg ctcgaggccc tctccgaccc
    21181 gagcaatctc agcggactcg atgaaaacga tccccagagc atggcgcggt tcatgaagaa
    21241 gatggggcag gaaatgggcg aggatctggg cgatgacctc gatgcagcga tggaggatgg
    21301 gcaagcgcca gcatcgggga tcgacggaac ggattttgac tgacacgcac tgtaagaata
    21361 ttctcttgac aggatcagcc atgtctccta ccatcggcgt tgaaacggag gaagccgccc
    21421 atgttcgccg gagaacatct ctgcaaggtc gatgaaaagg gacgtttcct gatcccctct
    21481 ccgcttcgtg agcgattcca agccgagggt aaccaggtca tgttcctgaa gaacaccgag
    21541 cagacgctct ggatgtactc cgccaaggaa tgggaacagg tcctggaacg cacgagagcc
    21601 accctggacg aggaccagac ccgcctgttc atgcaccatg tgatggccca agccggcgcc
    21661 tctgacatcg ataaagccgg ccgggtgttg attccaagcc gactccgcaa actcgtcccg
    21721 atggatgagg atcaggaact cgtcctcgtg ggcatgtatc atcgtctgga aatctggggc
    21781 ccgtctgaat ggcggcgcta cctcaccaga aacgaagacc gggccgagca ggatatggct
    21841 aagatccaaa atcttctata ggcaggccct tgcgccgcgc atcccgtccc cgacaatccc
    21901 ccgttacgat ccgatccacg tccttcaggt cactgtccga gccggccgta cccatggaac
    21961 gagcaccgct cattcccaca cagtccaaca ggaatcgccg tgcgctgtcg ttccctaccc
    22021 cggcaggtac tggcttgagc gtcaccatcg actccatcac cctcacattg cccgtcccgc
    22081 caagcatcaa tcatcagtac gccacggtca acgggcgtcg cctgctctcc gcgaaaggac
    22141 gtacgtataa agactttgtc gggcaacaaa ttgtggttgc gcttgcccaa tcgcctcatc
    22201 gtgctactct aagacagacc ttgcaacagg cgagcctgtc tctctcgatc cattttttct
    22261 tcgcctctgc gctgcgacgg gatactgacg gcgggctcaa gatcgcgcaa gatgccctgt
    22321 gtgaggggct gggactgaac gacaatcgag tggtcgaaac acatctctat aagcaccagg
    22381 atcgcgacaa tcctcgcatg gaaatccggt tatcggtggc gccgtcgacg gcgcctgatc
    22441 cgttttttcc cgcctcactc aaggcacagg tcgcggtgat ccccaggccg ccaatcgaca
    22501 acgggcaaga ccgataggat ggttggggcg acacccgttt gaattccgcc tcgccggcgg
    22561 gcagcacgcg ctatgacata ccgacccatt acaattgatg cgctccgaat cggcatgcac
    22621 gtcgccaaga tcgacgtcgc ctggtttcgt tcccccttca tgcgccactc gttcctgatt
    22681 cgcactgacg agcagatcga gaagctgcgg cgcgcgggcg tgaaacaggt gagcatcgat
    22741 ccctctcgcg gactcgatct tcaagatcca atggccccgt cccaagcccc gctccctgtt
    22801 gccgcgcaac ctgcgacttc gcagatgggc aagtccgtcg agatccggtc tcttgccacc
    22861 atgacgcaag aattgctggc ggccaggtca gcgcgagcca aactcgagga atccgtccat
    22921 tcggcgttct cccgcatcgc caaaaccggt gtggtcgatc cggaggaggc cagccatgcc
    22981 gtccatgcca tcagcgcggt cgcccaggcc ctcaatactt ctgccttgtt catggtgttc
    23041 agccaaggcc gagaggccaa tgcgcccctc agtcagcatg cactggccac ctgcagtttc
    23101 tcgatgatcc tcgcccatgc ggctgactac aatctaatgg cgatccagga actggccacc
    23161 ggtgcgctgc ttcacgatat cgggctcctg caggtgccac ccgccatcct ccggcgcgtg
    23221 catgacacct cgaccacagt ctctgaacaa aaccgtctga cctatgaagc tcacgcgcgc
    23281 tcgggcgcca ttctcttgga acgccggggc ggattcacca aaccggtgga acagattgtg
    23341 gcggaacatc atgcctacct gaacggcagc ggctttccag cggaaaccgg cggcgcgttc
    23401 acgtcggaca tgacccggat cgtgatggtg acggaccgct acgacgaatt gatgacgggc
    23461 tttggcggag cctcgcccct gaccccgcat caatcgctcc agcggctcta ccaggaggga
    23521 caggaaggcc gatacgaaag ccggctgatc tccctcttcg tcaaagtcat gggcatttac
    23581 cccgtttaca gttatgtcca gctgacgacc ggcgaacgtg ccatcgtctc cgtcatcaac
    23641 tcaggcaaac tccaccaacc catcgtcacg atcacccacg acccttccgg agaaccgtat
    23701 atcgtcccgc tcgtcatcga cctcgccaat caggatgaag aggcccctcc gcgcggggtc
    23761 cgttcggtgt tgggaacaat cccggaagaa tttcaacgag cctatcactg acctcagccc
    23821 catccgtgtg ctcccctcat cgagaagatc ggaccgctgg agaagccctg gcggcatgca
    23881 ggcctattcg taacggagcg cttcaatggg attgagacgt gcagctttat tggcggggta
    23941 gagtccgaaa aagagcccga tagccaagga aaacagaaac gccgtcagaa cggcatcgac
    24001 tgaaatcatg gtcggccaac cggcaatcac cgtcgtgagt cgtgcgccga cgatgcccac
    24061 aatcacgccg atcaggcccc cgaatagact cagcgtcatc gcttcgatca ggaactgggt
    24121 caggatatga atgcgtttcg caccgacggc catacgaacc ccgatctcct tcgtccgctc
    24181 ggtgactgag accagcagga tgttcataat gccgataccg ccgaccagaa gagagacgga
    24241 ggcgatggca aacaacatga ccgtgagcgt ttgactggta ctttcctgca ccttcccgat
    24301 atcgacctgc gtcctgatcg taaaatcatc cgcttgatcc ggctccagcc tgtgcctcag
    24361 ccgtagcgtc tcccgaatat gctccacggc ctcggggaga tcgccgctct gttcggtgga
    24421 cgcgaagagc gccccgaccg atccgagaaa ctggctgccg aataccctcc gctccgccgt
    24481 cgtgaaggga atgaaaatca cgtcatcctg atccgtgccc tgggccgact gccccttcgg
    24541 agccaatact ccaacgactt gaaacggcac attcttgatg cgaattgtgg ctcccatcgc
    24601 ctcttcgcca ggatcaaaca aattctccaa ggtggtctgg ccgatcaacg ccacgcgagc
    24661 cgcactgtcc atatccgtct gggtgaaggg cccgccactc gtgaacgacc agtcgcgaat
    24721 cgtcaggtag ctcggggaga ctccattgac ggggccattc cagttccgat tcccgttgac
    24781 gatttgcatc acatcgcgct tcgcccaccc ggtgtcggac aggagcggac tccgtttttt
    24841 caattcggcc gcatcgttga tcgtcaacgt caccgccccg ccctgcgcgc ctcgcacacc
    24901 actgaccgtc gtcgacccgg gcatgatgac gatgacattt gtccccatac ttgccacctg
    24961 ggcctgaacc gccgccctgg ccccttgccc gatactcacc atggcgatca ccgctcccac
    25021 gccgatgacg atgccgagca tggtgagacc ggcccgcagc cggttccggc tcagaatccg
    25081 caacgccgtc atgacagtga gcagaacgaa tgccggcatc tggccctacg tgaccgcaga
    25141 taggtgaggc tcgcgccgga catcctgcac gatctgcccg tccttcatga cgagttcgcg
    25201 ggacgcatac gcggcgatat cgggttcgtg ggtcacgaga atgatcgtga tcccgtcctc
    25261 ccggttgaga tgttcgagga tatccataat ttcacggctc gacgcggtat cgagattacc
    25321 ggtcggttcg tccgcgaaca ggattgaggg ggcgccgacc aatgccctcg caatggcgac
    25381 gcgctgctgc tgaccaccgg aaagctgggc cggataatgc tgttcccgcc cggccaaccc
    25441 gactcgctga agcgcggcgg ccgcctgccg gcgttgctcc ttgatcgaga caccgcgata
    25501 aaacagcggc aattgcgcat tttccaatgc actggtccgg gggatcagat tgaaattctg
    25561 aaacacgaac ccgatctgcc ggttgcgaag atcggcgaga aggtctgggt tggccgtact
    25621 cacatccaac tcgtcgagcc gataccggcc tcgcgacggc ttgtccaggc aacccagtag
    25681 attcatcagc gtcgacttgc ccgaccccga cgtgcccatc accgcgacaa actcgccgcg
    25741 gtcgattgtc aaattgatgc cccggagcgc ctgcacctcg acatcgccga tgcgataaat
    25801 tttccagagg tcttcgcaga cgatcaaggg agccatcgtg ccctcacaaa cgggatcgca
    25861 cgcgactgtt tccaccatgg ctacatccca cggtcccgcc gcccgccgcg ctgttggccg
    25921 ccaccgaagc ccggtgggag ttccccgctc ttgcgctcac cgcgtggaga ctcgataccg
    25981 gtcacaacct ggtcgccttc ctggataccg gcagcagtga tctcgacata ggaccgatcc
    26041 gatataccca tctcgaccgg aacgcgctcc aactctcccg caccatccag cctccagaca
    26101 ccccattgcc ggttccctga ttcaggagac ccactcgcga tcgtaggcct gccgccggcg
    26161 cgctctccat ccctcctcgg cccggcctct cccttctcat ctcgaacggc cttggggggc
    26221 acgaagcgaa gggccgcgtt cggcaccttc agcacattct cccgcttgtt gacgacgatg
    26281 gagacattcg cggtcatacc tggtttcaaa cgaaactcag gattctcgaa ttcgacgaca
    26341 acgtcatagg tcaccacgtt ttgaatattg atgggcgcat tgcgaacctg gcgaacccgc
    26401 ccttgaaagg cttctccggg ataggcatcc acggtgaatg acgccgcttt gccgtcgaca
    26461 atgccgccga tgtcggcctc gctcacattg gtatcgacct gcattttggt cacatcctcg
    26521 gcgatcagaa agagggtggg aatggaaaag ctggcagaga tacgctgacc gacttccacc
    26581 agacgggaaa ttactactcc gtcgaccggt gagcggatga ccgtatactt caggtccagc
    26641 tcggccgctt gtaacatggc ttccgcctgc ttcacggcgg cttccgtcac tttcagttgc
    26701 gcgacggcgc cctcggaagc ggtcagggca acatcgactt cattctgcga aatgaactgc
    26761 tggccgataa gcgactttgc tcgatccagt tcgcgccgac gctgagccag atcgatccgc
    26821 gccttttcaa acgccgcctt ggcattggca aggctggcga ccgcttgatc acgcctggcc
    26881 tggtagggga aaggatcgat ccgtgcgacg acctggttcg ccttgacctt ggaattgaaa
    26941 tcggcatgca ggctctcgat catacccgag acctgactgc caacctggac cgtcgtaatg
    27001 ggattgatgg taccggttgc ggtgaccagc gagacgactg atcctcgctc gacgggaacc
    27061 gtgcgaaagc gcagaggaac cttccgctcg ccgttgaaaa acacgtaacc ggcgatggcc
    27121 aggccgagcg caagcacacc gacaatgata ctgacacggc gcatgaacag gcgttcctct
    27181 tcgcccaggt actgcgggca ttctagcaga aaacaatttg acgatgtcag gcagagggcg
    27241 gcgatggggc ctctgagcgc ggagcgaacc gaaagagaac ggccgtttgc cagaatggca
    27301 aacggccgtt catgtcacgt gatccaccag cggtggacct gctcactggt gcttaggggc
    27361 tgacggtaat gcggtctttg atgtcgtcga acacatcctt cgtcaccaca ttcttggcga
    27421 cgagttcgcc cttcactctg tagggacggt tactgacgat gcggtctgcc acatcctgtg
    27481 taatacccag cgtgagcacg agatccgagg acggcgcctt gttgatattg agcagcggcg
    27541 cagccggtgc ctcaaatggg gccggcgcag gagcagcttt cgaagaagca ccgccgccgc
    27601 ccagcggcgc ggtcaaggcc ggcgactcca ttcccgatga cagggaacgc gaccggtctt
    27661 tcatctcttt ctgataccgc gcgaccaatg acttcaattg ctcattgtgg cggcgagctt
    27721 cttcatactc ctgcttgacg ctgcggtttt gcgcgctcag ctgcttgacc ttgtcttcca
    27781 gctccttggt cctgacatcg atggtatccc gctctttatc gcgcccgtgc tggatacgct
    27841 ggagttcatc acgggcgatc tggctttcgg aaccgaactt cgcgttcaaa tcctttaagg
    27901 tcttgacctg ttgctccagg gcatttttct gcgctcgcgc gcgctccagt tctcccctgg
    27961 tggcatcggc atcggcaagc gcctcatgat atttcttgtc acttacgcac ccgctgacgg
    28021 ccatcgcgct catgagcgca agcccagcca cccacacatg cctcatgcga tcctcccctg
    28081 ttcgatctgg accgatcgga cctcttctct cttgttgtgt gtatgccatc acttcaactt
    28141 tgtcaacaag tttgccgatt gc
//
LOCUS       DAOQEW010000025        28087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_384_length_28087_cov_9.119506, whole genome shotgun sequence.
ACCESSION   DAOQEW010000025 DAOQEW010000000
VERSION     DAOQEW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 28087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28087
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_28087_cov_9.119506"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="argB"
                     /locus_tag="PLS35_04675"
     CDS             complement(<1..698)
                     /gene="argB"
                     /locus_tag="PLS35_04675"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNI17983.1"
                     /translation="MNKLIKKADVLIEALPYIRTFKGKTVVIKYGGHAMTDASLKARF
                     AQNVVLLKYVGLNPVVVHGGGPQIDQMLDRLGMEAKFKHGVRVTDAATMEIVEMVLAG
                     RINMEIVDLLNRHGGQAVGLSGKDGGLMLTKPLTAKAWAESIEKDIDVEDRDADFGFV
                     GDVKSIDPTLLLKLQEDHYIPVIAPIGTDREGNTYNINADLVAGAIAAALKAEKLVML
                     TDVKGIRDANGRHLS"
     gene            complement(728..2131)
                     /gene="hslU"
                     /locus_tag="PLS35_04680"
     CDS             complement(728..2131)
                     /gene="hslU"
                     /locus_tag="PLS35_04680"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNI17984.1"
                     /translation="MTTDSTSRSLNVNNLTPRQIVEALDRYVIGQQDAKRMVAIALRN
                     RWRRQQLAPELRDEVMPKNIIMIGPTGVGKTEIARRLAKLAEAPFIKVEASKFTEVGY
                     VGRDVESIIRDLTEQAISLVKTKHLEDVQEKASRLGEERLLDLLLPGGPSRPASAGFD
                     HAGSRAEAAEPTESPDATRSKLRLQLRDGKLDQRSVEVEVKERALPLGVISNQGGMED
                     LEGNLRDMLGGMFQGKKKKRVMKVPDALKHLTQEEAQKLIDMDEVVREAITKVEQTGI
                     VFLDEIDKIAGRERAMGPDVSREGVQRDLLPIVEGSTVSTKHGAVQTDHILFIAAGAF
                     HVAKPSDLIPELQGRFPIRVELAPLTKEDFVRILTEPRGALVRQYHALMATEGLTVEF
                     TDDGLAEIAATAVQVNERTENIGARRLFTIMERLLEQVSFEGSELEQKAVVVNAAYVR
                     ERLLNIVKDQDLSRYIL"
     gene            complement(2145..2681)
                     /gene="hslV"
                     /locus_tag="PLS35_04685"
     CDS             complement(2145..2681)
                     /gene="hslV"
                     /locus_tag="PLS35_04685"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNI17985.1"
                     /translation="MKIRSTTVLCVRRGNQVTMGCDGQVTVGTTVMKHNARKMRRMHN
                     DTVLSGFAGATADAFTLFEKFEAKLAEYRGNLTRAAVELAKDWRTDRVLRRLEALLAV
                     ADLDHSFIISGTGDVVEPEDGILAIGSGGPYALASARALLAHSELGAEPIVKESLMIA
                     GGIDIYTNQQIVIESLTR"
     gene            complement(2678..3640)
                     /locus_tag="PLS35_04690"
     CDS             complement(2678..3640)
                     /locus_tag="PLS35_04690"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNI17986.1"
                     /translation="MDRAIRAFLDVLAVQDDASPQTIRAYTSDLAQFRAFALAALKPR
                     DTLTVESIEAGLIREFLASRDRKGEKKTSLARKLACLRSFFRYLVRVGRVQANPAEDV
                     RAPKLPKHLPQVLTKDDAGALMEFPPGDAREGLRDRAILETLYSTGARVSELVGMDRQ
                     DISRSEGVVRVRGKGGKERVIPLSPLALEAIDAYHAQASVTAASNSSDRGAPGGSPVF
                     RNRRGGRLTTRTIARIVAKYSGQLSGGAIHPHTLRHSFATHLLDEGADLRAIQEMLGH
                     VSLSTTQKYTHLATDQLLALYDRTHPRAGAAGEAEQGNKQKGSR"
     gene            complement(3613..4929)
                     /gene="trmFO"
                     /locus_tag="PLS35_04695"
     CDS             complement(3613..4929)
                     /gene="trmFO"
                     /locus_tag="PLS35_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNI17987.1"
                     /translation="MREDIVIIGGGLAGSEAAWQAAHRGAKVTLYEMRPKETTKAHKT
                     GDLAELVCSNSLGSMDPLNAPGILKGEMRRLNSLVIRAAEEARVPAGSALAVDREIFA
                     RAITQALEGHPNIRIIREEVTEIPADAVAIIATGPLTSEKLSKAISELTHERHLYFFD
                     AISPIIDADSINMDIVYRASRYGKGGADYLNCPMDEAAYNALYDAMMAAEKVQPKEFE
                     KVAYFESCIPIEVMAERGRQTMQFGPLKPVGLDDPKTGRRPYAVVQLRTENVHGTCYN
                     MVGFQTKLTYPEQKRVFRLIPGLEQAEFLRFGSLHRNTFINSPQLLRDTLQLKTRGTV
                     FFAGQLVGVEGYTESAAMGGIAGINAARGLAGQPLVTPPPTSAHGCLMAHITKSDPAH
                     FQPMNTNFGLFPPVTVKTRDKDQKRRLIQQRAVEDFDAWIAQSGLS"
     gene            5115..5189
                     /locus_tag="PLS35_04700"
     tRNA            5115..5189
                     /locus_tag="PLS35_04700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5147..5149,aa:Val,seq:cac)
     gene            5750..6079
                     /locus_tag="PLS35_04705"
     CDS             5750..6079
                     /locus_tag="PLS35_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17988.1"
                     /translation="MRGTLATIMLGLLLTTGCSTTNQVATQDQNYNAPLSIYSAMDST
                     SLVYSDPMAASQANDNPYRWLGFVLHPVGHLVDYTVNRPLYAIARHAPYLFGYTAEDS
                     MIDSQRR"
     gene            6230..7375
                     /locus_tag="PLS35_04710"
     CDS             6230..7375
                     /locus_tag="PLS35_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17989.1"
                     /translation="MHRRLSLLPALLTMFALLTGCETPPPARAPLPPSESDLDLLKST
                     KLCDTRKVFQEHHPGIVPKTQAWGSGQELRFSAEQSLSKGEESYFFDEDGTLVGTLFI
                     FRSGLDLGPYKTLRYTLTQLKPSLEFYLTVAQLADRQNMASSTLYDTGDEKTTTRYLV
                     LGDRKNPQLLAASFSIDPYVKLFSPYRKEFLDRLRDTGQQSGGQHLDTQGAEDKEPFA
                     SLQQFARGQTAQLAYCGAKNQTIALDAYQKASASGFTSQVWQAELHHRLGVSWEAAGD
                     LEKAKRELLASLAQRPNSPEVVNNLGYVYGKLGEKQNALASFEKAVLMRPNYAIARFN
                     LAEAIAEANPRRAINEYETYLALTEGIPEEADRSALAQSRVKALKHQ"
     gene            complement(7442..7849)
                     /locus_tag="PLS35_04715"
     CDS             complement(7442..7849)
                     /locus_tag="PLS35_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNI17990.1"
                     /translation="MKTSAKKSTPTRKKPAKANGVKYPDIQKDLEGQRAAILAEAGVV
                     LTNPTGLEVFPDVSDQASAEADQHFSFRIRERERKLLKKIDEALGRLATHTYGICEGC
                     QGDIPYKRLKARPVTTLCIECKTSQEEAEKSPR"
     gene            complement(8010..8612)
                     /gene="recR"
                     /locus_tag="PLS35_04720"
     CDS             complement(8010..8612)
                     /gene="recR"
                     /locus_tag="PLS35_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNI17991.1"
                     /translation="MSIDQQGLLAKLVRELVRLPGIGQKSAQRLAFHLLKAEREDALR
                     LAEAIQAVKDGLSFCRQCRNIAEGELCEFCRDSKRDRSKILVVEEPSTLYAIERAGGY
                     RGLYHVLLGVLSPLDGVGPSDIRAEELLERVKAGGVEEVIVATNPTIEGEATAIYLTR
                     LLKPHHVRVTRIAYGIPVGMDIEYADEVTLVKSIEGRRDL"
     gene            complement(8691..9014)
                     /locus_tag="PLS35_04725"
     CDS             complement(8691..9014)
                     /locus_tag="PLS35_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNI17992.1"
                     /translation="MKSPFGNMSNILKQAQAMQEQMAKVQEQAATKTVSGTAGGGIVT
                     VTVNGAMDLLSVKIDPEVVKSGDVDMLQDLVVAAGNDALKKSRDMMAEEMKAVTGGMK
                     IPGLF"
     gene            complement(9011..10891)
                     /gene="dnaX"
                     /locus_tag="PLS35_04730"
     CDS             complement(9011..10891)
                     /gene="dnaX"
                     /locus_tag="PLS35_04730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNI17993.1"
                     /translation="MDYQVSARKYRPGTFDDVIGQSHVVQTLMNSIATKRIAHAFLFS
                     GTRGVGKTTVARILAKALNCEQGPTGSPCNTCANCQEITQGTSVDVVEIDGASNTSVD
                     DVREIRENVKFTPFRGQYRVYIIDEVHMLSNSAFNALLKTLEEPPSHVVFIFATTEIH
                     KIPATILSRCQHYNFRRISKAEIVQRLRHVADQDGLTIEDRSLMALARASEGSMRDGL
                     SLLDQVIAFGGKTIRHHDLETLLGAVPQERVRAMVEAVIDQNSAKALQVIAGLLDQGH
                     DVRAYCADLVEYVRNMLVAAVVPAGPELRGLIEVTDEDLTQLARDAEQFSVEHLQELF
                     RLWAAAEDHLRVSAHPRFVLETAAVRATRLLRAPESPAGLPGVNVQSSATPVDRATGT
                     RTSMSSSEKVVSPKPTKRDTVKSSSDAGDKPVVPSASAPAPAPVARPREAVVPSSQTP
                     APSIPSAQASATAQVPVESASPKPEPPSHRATASPSAEINWEEFQEAVSGQHPNIAPF
                     LEMGRVVKIEGGLVTLGFTKQATVARAMLEKEDNLQSLAALGERIYGTALRVRIIEVA
                     EQDPEATPTMKQLRVAKEQEQRVILTQQAKAHPLVKQALEMFGGELAEVRTTTPAQEV
                     QE"
     gene            complement(10993..11092)
                     /gene="ffs"
                     /locus_tag="PLS35_04735"
     ncRNA           complement(10993..11092)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS35_04735"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(11386..11475)
                     /locus_tag="PLS35_04740"
     tRNA            complement(11386..11475)
                     /locus_tag="PLS35_04740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11439..11441),aa:Ser,seq:gga)
     gene            11702..13810
                     /locus_tag="PLS35_04745"
     CDS             11702..13810
                     /locus_tag="PLS35_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNI17994.1"
                     /translation="MKILVGIGVVILLFLILIIALPFLIDLNKYQDRYRPLIEEALNR
                     KVELQDIRLTIWPRIGARIGGFVVQDDPAFRTGPFASLASLDVGVKLLPLLKGRVDVE
                     EITLRDPVILVLKNSQGQLNISTIGAKPTAPAAPSKPEAPGQPAGSPLQALALFAVDR
                     VSIDGGKLTYRDESTAKPTEYTVTQLEFLLTSVHLGDSPTVHLGATVLPYNLPVHLDG
                     TFGPLVETLDVKSFSFNLGLGKVAVGLKGRAVGGNLDATINALQIDTADLPIALPLTK
                     PVQIKDLHLTLHALYPIPPDTPPASQVDLTDLGLTLVMGGSAINIKGTATKGLANITA
                     ASASINSSDLPVAVPLAKPVEVKDLHMNVRAKYPPKEGTAPLELAEVPNLGLTIALGS
                     SRVEVKGSVLGGLAKVTATSKLVNSADLPVALPLKKPVEIKDLQAAAEMKGQEARLTN
                     LTLQVFGGFLRTQGTLGLGTPTPPFTGTVALQGLQVGPALQAVGTDQVSLSGTANAEL
                     ALSGRGFTHPDLVKALAGTGRMSVREGKIEGINLLQQAAMLLKVVGVSLDNVKATAFS
                     TIESDVVIKQGLIGLQRVLIDSHDFQATGGGTIGLDQSLDMKLNLNLSQALSQKIAAG
                     SPIAKVAMTNGRLSLPLVITGSTQAPSYGLDTKMFAGKVQEQVKEKVKGAVGDLLKGS
                     AKPEDLKKQGKDLLKGLFGR"
     gene            13828..14628
                     /locus_tag="PLS35_04750"
     CDS             13828..14628
                     /locus_tag="PLS35_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNI17995.1"
                     /translation="MSLANLITQYVVQYGFRIMGAILVIVAALFVANSVGRLFERWLN
                     EQDLEPPLRLLLLRLLKALLVILGVLIALDQLGVQIAPLVAGLGVAGLGVGLALQGVL
                     SNVVAGLSIIFTKPYRVGEHISLLGVHGDVAKIDIFTTTLLHPDHSRIVIPNRKVVGE
                     ILHNFGTIRQLDLSIGVSYQTDIETTLRVLRELVSRHPKVLQDPVPIIGIAAFADSSI
                     TLSIRPWVPVASVGSAHIELNQAIIQRLRADGVDIPFPQREVRLLNPS"
     gene            14767..15381
                     /locus_tag="PLS35_04755"
     CDS             14767..15381
                     /locus_tag="PLS35_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17996.1"
                     /translation="MSRQTDLDRTRRSERTPTTLAETETETALADDPTAGNNLDKIRD
                     ILFGAQVRDHERRFTKLETQLLAEAAQLRNDLKDRFASLEQYIRTEVEQVTGRLNAEE
                     QGRTKAVSALTAELHTLAGVLQQTATQLREQSEQADRDLRDQLQHQAASLTGEFTQQH
                     ATLSATLDQAVQQLSHQKTDRTSLATLFQELSQRLSHDQPPQQA"
     gene            15498..17228
                     /locus_tag="PLS35_04760"
     CDS             15498..17228
                     /locus_tag="PLS35_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI17997.1"
                     /translation="MAQPPNRSTPPAEHDYAELRSLLLAPEQTRLEQLQEQVEHIDLN
                     AKNLSRVLPDAIALRGEGDHQLTHVLTPHVSEALGASVRRQPHMIVDAIAPIMMPAIR
                     QAIANALRGMVQSLNQTIEHSLSIQSVKWRVEALRTGKPFAEIVLLHTLRYRVEQVFL
                     IHSQTGLLLAHAAGDAVAVQDQTLVSGMLSAIRSFVQDSFGADPEQVLNTLQVGELTV
                     WIEQGPAAILAAVIRGTPPESLRLHLQDTLTRIHAERADALARFTGDAATFASVTPLL
                     EECLRAQFESRRRGIAPMTWVLIIGALLVTTWWALSTYQERQRWQSYLSRLATEPGLV
                     VTDAEPEGGRYRLTGLRDPLAADPAILMQDSGIDTSRVVAKWQPYYALDTSFILKRAG
                     FALSPPPTVHLALEGSHLTAGGTASADWIRQSRSVARAIPGLDGYDDRRLVSQSLDML
                     RQQFTETSILFEQGSSNLVSPEQLQQARRIVGMLRQLEDLARLASAAVTVSIVGQTDV
                     VGGTSQNRRLSDGRAKAVLDAIHPASFPALTFHTRGMAQEAELQRSTPAVAHPQHRRA
                     SLEITIQPMP"
     gene            17239..17775
                     /locus_tag="PLS35_04765"
     CDS             17239..17775
                     /locus_tag="PLS35_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNI17998.1"
                     /translation="MIEKKICMLGAFAVGKTSLVRRFVSSCFSDQYQTTIGVTVDKKS
                     MTVDGTSLTMVLWDLYGEDEFQKLRSSYLRGSSGYLLVLDGMRRATLDIALHVQERAS
                     DTLGPVPFVVLINKHDRRAEWDITDQDLAQLAQRGWSVVMSSAKTGQGVEEAFTTLAR
                     AMLTPSNSSAAAGRPHGS"
     gene            17765..19555
                     /locus_tag="PLS35_04770"
     CDS             17765..19555
                     /locus_tag="PLS35_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI17999.1"
                     /translation="MAPDPSLRTPDFLCSLFERLEYVVLEHVAGTQFRILGHPSPWLF
                     RLYPEAEASLQLSVDSRTPVLHNFLADAAEVWQTGDAHIGRSGFWTEQTAFDVPWHFE
                     ALALREGTSRLLVIQHSTAAYEQQAAMLQRAREQALQQHEQERGHQRTQQDLTMQLVD
                     MERARDDVAAILQDLGLATLLIDQEGHVRFVSASAASLLERPSASIKPDLRWETLLPV
                     TKADRLTVQSLLEHPIPQRERVCCHIDTPAGRRLWLDLELHDDPRDRRSKIVFLHDMT
                     DVYHLRRMLELKAHYHDLIGKSRGMTQIYEQLQDLARVDSTVLIEGETGTGKELVARA
                     LHQASARRTGPFIAANCAGLTDSLLGSQLFGHKKGAFTGAIDDQQGLFEAAQGGVLFL
                     DEIGDIPHTVQTNLLRVLQEKEVTRLGETKPRKINVRVLTATHHNLSQDVAKGTFRAD
                     LLYRIRVARIHLPPLRERKEDIPLLVTSFLAEGRASMGKLIHRASPATMAALMDYQWP
                     GNVRELKSTIECAMIHCKGDSLEATDLPPELQREPTALTPEPITTDERSRFVSALGQA
                     RGNRTKAARLLGMSRATFYRRMTELDLPAN"
     gene            19818..20129
                     /locus_tag="PLS35_04775"
     CDS             19818..20129
                     /locus_tag="PLS35_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI18000.1"
                     /translation="MDKVFPFSTNRPDQPTPREQEILNYIWAGLTSQEIAARLQIAQK
                     TVESHRANLLRKFRATNAAQLLRLALLEGLLSVPSTERAPDVKRVVQSAKASRRLTSI
                     K"
     gene            20516..21628
                     /locus_tag="PLS35_04780"
     CDS             20516..21628
                     /locus_tag="PLS35_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNI18001.1"
                     /translation="MMRRYACFFLALLIVALTAYGVNAESPTPTDTTAAAPTLDSVTL
                     KDGTVIYGRVLGMIADELHIKTGFGPTAGDDIVKIMWPNVAKLVVNRPIPFTLKEGTT
                     VVGTAQPGEPGTLTLQAAPMGTPMAIPLDAVVGMNQPAVIYTGALQAGFSQTTGNSHL
                     RNGSLLGELSARSESLRLTILGRYIYGDNSGNLIVRNSRGTIKLDFFLTKRLYWFASA
                     YFEQDTFQDLKLRTALATGPGYQLLDRGDLTGFFKDMTLWAEAGAAYFNEDFKLADDK
                     SSTRGRWAVKWNWPLWGGDQVSLYHFQEGFQSLANSKDLYLTADTGLRFKVWGGLVSG
                     FQWTMRYNKNPPPGVSDTDNLYLITLGYSFDTSRKQ"
     gene            21679..22104
                     /gene="mscL"
                     /locus_tag="PLS35_04785"
     CDS             21679..22104
                     /gene="mscL"
                     /locus_tag="PLS35_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNI18002.1"
                     /translation="MGMMSEFKEFAVKGNVLDMAVGVIIGGAFGKIVSSVVSDILMPP
                     IGLLMGHMDFSSLFIPLSEEAKGKSLAAAKAAGAATINYGVFLQTLLDFTILAFVIFL
                     VVKQMNRFKKATPPGPPPAPPKEEVLLTEIRDLLKNQRH"
     gene            22173..23375
                     /locus_tag="PLS35_04790"
     CDS             22173..23375
                     /locus_tag="PLS35_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI18003.1"
                     /translation="MKHVCVVPVLLSAALLAACTSTPAPNDSASRALNEMKAVARPIP
                     PKPDPRDLRIAELERQKTDLEGELDRLRSSSAADSDRSKARITELENQLNQRDRELAG
                     LRNAAGDKDRLAGQLSDADRRLSAKDQELAALKQSAADKERLAAELAALQGLLATKDQ
                     ELAGLKHNAGDRDRLSSELAQAKQHIADLERQLSGKDQELGTLKGAAGDRDRLVADLA
                     AAKQRASDLENEIGRRDHEMAGLRGALDQQKTSLAEAKNDLSKLLQAEVSKGNVTMKQ
                     LGDQLTLGLATTLLFDSGEATLKPGGADVLNRIGSVLKNYPDRSIHVAGHTDNVPIKG
                     RLAKRFPTNAELSQARAESARQALTEGGMAAEKISAKGHADSRPIASNSTAEGRQKNR
                     RVEIVVGQ"
     gene            23622..25073
                     /locus_tag="PLS35_04795"
     CDS             23622..25073
                     /locus_tag="PLS35_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI18004.1"
                     /translation="MRRAVWALMVLGALASACETVPQPQPVAAPPQVGDAKLLALQQE
                     REQLLTTLGEFHDRIRDLESKLGDRQNQPAAASYDQLLNAKDAELAELRRLSPERDKL
                     TSQLAMANNELLQARQRIGSLEQQLATREKDLVALQGRSTAISDLEVARRRIMDLEAQ
                     VARQDHDLRTIKSENAERDSLAAQLQTATATIDSLKARISALDQQLRDREQAFETVRS
                     RLMERDKLVPQYNAMIAEIYQARHRIAALEQRLNEKSRDLSPRQKGAQAATAPRETGA
                     TSNKNAGSSEKDAGRTGSATPTSPSQSTSREASFAAMKEEILKVLPSDGGQKAITVKQ
                     DGSRLTIALSSNWLFASGEAALTSEGMTMLKRIGTVLGPLSDKFVQVAGHTDNQALSK
                     TLQKTFSDNKALSWARAENARRALVTGGMSPERTKAVGLADSRPIASNATEQGRQKNR
                     RLELVIVQGPTVASTTGKTTEERDRLAALAPSY"
     gene            complement(25095..26336)
                     /locus_tag="PLS35_04800"
     CDS             complement(25095..26336)
                     /locus_tag="PLS35_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI18005.1"
                     /translation="MRDISRRRFLQISAMSGGVLLLGDLAERILNVPLLRSAALAAEP
                     IKIGILDPLSSPYKTSSIHDVHGANVAVDLFNKRGGVLGRPVTIIEADDASSPDAALK
                     AATKFVQDDRVDVLMGTFNAECALVVSEYAKKANKLFMVTGAHLPELTGAACNSHTFV
                     FMPNASMLAQAVVPQLVKAYGTRWYMITTSSLDGKAMAQAMASTGLTHGVEFVGETLM
                     PFGSTDFTSALSAAKDKRPTLVVLNLYGWDLVHALKAYTKLELAKDKIGVGGMIAGEQ
                     IGRPLGYANNAGIWGLIWDPKVNTDGSKRFIQGVVEKYNHTPTSRCYLGYAAMTQILE
                     AIQRAGTTEASALIKVLEGHEFDGLKEGRSYFRASDHQHVQDVLVGEAYGKELGLGHY
                     KILATVAGESVATVQSGSCQL"
     gene            complement(26610..27695)
                     /locus_tag="PLS35_04805"
     CDS             complement(26610..27695)
                     /locus_tag="PLS35_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18006.1"
                     /translation="MKRQSVTAALGCLILLCGFPLSSEAYRDYFTDAQKQELGKIQTV
                     LIEALALTDKGAGNADGILDVAMKRMGELGYTTVSDPHVSHDVVVRVKCEQKKTWEGT
                     ASAGGDNDLPDAPSRLWKGPACQITYALGGMKVKWQKEVRTEFEDAVGAAQSAQAPDP
                     GLYAMNALHDALETYEFPLLLTAEWGQPDRLLKLLDSPQTSQIRKLKIISLLGEMVAD
                     EALPHLTEALKDKDLAKQAAVALGNMGKEGIPVLIDILKHSKQPELQAAAAKGLGDLG
                     NIHSDSRVVTPLLEMLDAPGIDIVVQTEVAWALGRVPDRRSVEPLFALDRKLQKIRKD
                     PPDPQIKKLKEAVFWSIKQVYTEDQYS"
BASE COUNT         6015 a         8633 c         7704 g         5735 t
ORIGIN      
        1 gagagatggc ggccattggc atcccggatg cccttcacat cggtcaacat cacgagcttt
       61 tccgctttca gcgcggcggc gattgctccg gcgaccaggt ctgcattgat gttgtaggtg
      121 ttgccttccc gatccgttcc aatgggtgca atgaccggaa tgtagtgatc ttcctggagc
      181 ttcaacagta aggtgggatc gatcgacttc acgtctccga caaacccgaa atccgcgtcc
      241 cggtcttcga catcgatgtc cttttcgatg ctttccgccc aggccttcgc cgtcaacggc
      301 ttggtcaaca tcaagccgcc gtctttgccg ctcagtccga ccgcctggcc gccgtgccgg
      361 ttgagcagat cgacaatttc catgttgata cggccggcaa ggaccatctc gacgatttcc
      421 atggtcgcgg catcggtcac acgcacgccg tgcttgaact tcgcctccat gcccaaccga
      481 tccaacattt gatcgatttg cgggccaccg ccgtgcacca cgacagggtt caagccgacg
      541 tacttcagca acacgacatt ctgcgcgaac cgcgctttga gcgaggcatc tgtcatcgca
      601 tgccccccgt acttgatgac gaccgtcttg cccttgaacg tccgaatgta gggcaaggcc
      661 tcaatcagca cgtcggcttt cttaatcagt ttgttcatgc gtcactcctc gctcgcgcta
      721 gtcccgctta caaaatatac cggctcaggt cctgatcctt cacaatattc agcagacgct
      781 cgcgcacata cgcggcattc acaaccaccg ccttctgttc caattccgag ccttcgaacg
      841 acacctgttc gagcagccgt tccatgatcg taaacagccg ccgggcgccg atattttctg
      901 tgcgctcgtt gacctgcacc gccgtcgccg caatttccgc caagccatcg tccgtaaact
      961 cgacggtgag cccttccgtg gccatcaacg cgtgatactg gcggactaac gcgccccgcg
     1021 gttcggtcag gatacggacg aagtcttctt tggtgagcgg cgccagctcc acacggatag
     1081 gaaaccggcc ctgcagctcg ggaatgagat ccgatggttt ggctacgtgg aacgcccccg
     1141 cggcgatgaa cagaatatgg tcggtctgga cggcaccgtg tttcgtgctg acggtagatc
     1201 cttccacaat cggcaggagg tcgcgttgta cgccttcacg ggaaacatcc gggcccatcg
     1261 cgcgctcacg gccggcgatt ttgtcgatct cgtctaaaaa gacgatgccg gtctgctcta
     1321 ctttggtaat ggcttcccgc accacctcgt ccatatcgat cagtttctgc gcttcctcct
     1381 gagtcagatg cttcagggca tcgggaactt tcatcacccg cttctttttc ttgccctgga
     1441 acatgccgcc cagcatgtcg cgaagattcc cttcgaggtc ttccatcccg ccctgattcg
     1501 agatcacacc gagtggtaag gcccgttcct tgacctcgac ttctactgat cgttgatcca
     1561 atttgccgtc gcgcaattgc agtcgtagct tggagcgcgt ggcatccgga gactcagtcg
     1621 gctctgcggc ctcggctcgc gacccggcat ggtcgaatcc tgccgaggcc ggacgggatg
     1681 gcccgccagg taacagcaaa tcgagcagcc gttcttcccc gagccgtgaa gccttctcct
     1741 gcacatcctc cagatgtttc gtcttcacca gactaatggc ttgctcagtg agatcccgaa
     1801 tgatggattc gacatcccgg cccacgtagc cgacttcggt gaactttgag gcttccacct
     1861 tgatgaacgg ggcttcggca agtttcgcca gccgcctggc gatttctgtt ttgccgacac
     1921 cggtggggcc gatcatgatg atgttcttcg gcatgacttc atcacgcaac tcgggggcca
     1981 gttgctgacg gcgccatcga ttgcgtaagg caattgcgac catccgtttg gcatcctgct
     2041 ggccgatgac atatcgatcc agggcctcga cgatctgccg gggcgtcagg ttattcacat
     2101 tgagggaacg ggacgtggag tcggtggtca tgacagggca gatcctaacg cgtgagcgat
     2161 tcgatcacaa tctgttggtt ggtataaatg tcgatcccgc cggcaatcat cagcgattcc
     2221 ttcacgatgg gctcggcgcc tagttcggag tgcgcgagca gggctcgcgc ggaagccagg
     2281 gcgtagggtc cgcccgatcc gatggcgaga ataccgtcct ccggctcgac cacatctccc
     2341 gttccggaaa taataaagga gtgatcgaga tcggccacgg ccagcagcgc ttcaagacgc
     2401 cgtaacacgc ggtccgtccg ccagtcttta gctaattcca cggcggctct cgtcaggttg
     2461 ccgcgatact ccgccagttt tgcctcgaat ttttcaaaga gggtgaaggc atcggccgtg
     2521 gcaccggcga atcccgacaa gaccgtgtcg ttatgcatcc ggcgcatttt cctggcgtta
     2581 tgtttcatga cggtggtacc gaccgtgacc tgtccgtcgc accccatcgt cacctgattg
     2641 cctcgtctga cacagaggac tgtcgtcgat cgaatcttca tcgcgaaccc ttctgcttat
     2701 tcccttgttc cgcttcaccc gcagcgcccg cgcgcgggtg agtgcgatcg tataacgcga
     2761 gtagctggtc cgtcgcaaga tgggtgtatt tttgagtcgt actgagcgac acgtgcccca
     2821 gcatttcttg aatagcccgc aggtctgcgc cttcatccaa cagatgcgtg gcaaaagaat
     2881 gtcgcagcgt gtgagggtga atcgcgccac cgctcagttg tcccgaatat ttcgccacga
     2941 tgcgggcaat cgttctggtc gtcaatcgcc ctccgcggcg attgcgaaaa acgggcgagc
     3001 cgcctggcgc ccctcgatcc gaagaattcg aagcagctgt gacggacgcc tgcgcatggt
     3061 acgcatcaat ggcctccagg gcgagtggac tcaaggggat cactcgctct ttccctccct
     3121 tgccgcgaac acgcacgaca ccttcactgc gactgatgtc ctgccgatcc atgccgacca
     3181 attcactgac acgcgcgccg gtggaataca gagtttcaag aatggcacgg tcgcgcaaac
     3241 cctcgcgtgc gtctcccggc ggaaattcca tcagcgctcc ggcatcgtct ttcgtgagca
     3301 cttgaggcag gtgtttcggc agtttcggcg cgcgcacatc ttccgccgga ttcgcctgca
     3361 cgcggccgac gcgaaccaga taccgaaaga aacttcgcag gcaggctaat ttcctggcga
     3421 gagaagtttt cttctccccc ttgcgatcac gcgaggcgag gaattcacga atcagacccg
     3481 cttctatcga ttcgaccgtt aatgtgtcac gaggcttcag cgcagccagg gcaaaggcgc
     3541 ggaattgagc caggtcggac gtatacgcgc ggatcgtttg cggagaggcg tcgtcctgaa
     3601 ccgccaacac gtctaggaaa gcccggattg cgcgatccat gcgtcaaaat cctcaacagc
     3661 tcgttgctgg atgagacgcc gcttctgatc tttgtcgcgg gtcttgacgg tgacgggcgg
     3721 gaacaggccg aagttggtat tcatcggttg aaaatgtgcc ggatcgcttt tggtgatgtg
     3781 cgccatcaga caaccatgag cgctggtagg cggcggggtc acgagtggtt gaccggcgag
     3841 acctcgtgcc gcattgatgc cggcaatccc acccatcgcc gcagactccg tatatccctc
     3901 gacaccgacc agttggccgg caaaaaagac ggtcccgcgg gttttcagct ggagcgtgtc
     3961 gcgcaacagc tgcggggagt tgatgaaggt gttgcgatgc aggctgccaa accgcaaaaa
     4021 ctcggcctgt tccaggcctg gaatcagccg aaagactcgc ttctgttcgg ggtaggtgag
     4081 cttggtctga aagccgacca tgttgtagca ggtgccatga acgttttcgg tccgcaattg
     4141 cacgaccgca taagggcgcc ggccggtttt cggatcgtcc aagccgaccg gtttgagcgg
     4201 cccgaactgc atagtctgcc gcccccgctc agccatgact tcgatcggaa tacagctttc
     4261 aaagtaggcg actttctcaa actctttcgg ctgcactttt tcggccgcca tcatcgcgtc
     4321 gtacaacgca ttgtaggccg cctcatccat cggacagttc agatagtccg cgccgccctt
     4381 cccataccgt gacgcacgat acacaatatc catgttaatc gagtctgcgt cgatgatggg
     4441 tgagatcgca tcgaagaaat acaggtgccg ttcgtgagtg agttcgctga tggctttgga
     4501 caatttttct gacgttaagg ggccggtagc gatgatcgcg acggcatccg cagggatttc
     4561 cgtcacctct tcgcgaatga tccgaatatt gggatgccct tccagcgctt gcgtgatggc
     4621 ccgtgcgaag atttcccgat ccaccgccag cgcggaaccg gccggcacgc gtgcctcctc
     4681 ggccgcacga atcaccaggg agttcaggcg acgcatctcg cccttgagaa tgccgggcgc
     4741 attgagcgga tccatcgatc ccagcgagtt ggaacagaca agttcggcca gatcgccggt
     4801 tttgtgcgcc ttcgtcgtct ctttcggacg catctcatag agcgtcacct tggctccgcg
     4861 atgtgccgcc tgccacgccg cttctgagcc ggccaaaccg ccaccgatga tgacgatgtc
     4921 ttcacgcatg aatgggaaac ctttgaaaag atggaaggag gctcattgta agaatcgcct
     4981 tttctcgatg tcaagaaaac caaggacgat ttagccttgg cggacgagct gagagctccc
     5041 tgcgtgagga cctatggccg tcgggcagtt ggttgctcct gtttgccgga ccgtggtaga
     5101 ctcccgtcct gctcgggcga ttagctcagt gggagagcgc ttccttcaca cggaagaggc
     5161 cacaggttcg atacctgtat cgcccaccaa tttttcaacc ccttccgaat aacactctca
     5221 atccctacaa cactgtggtc atttttgtgt cacggtgtga ccgtcggtcc aaaatctcca
     5281 ctccatctcg caggctttcc gggtaatgat gcgcatagcg ttgcgtcatc atcggcgact
     5341 tgtgcgcaat cgattaggcg cttgccggcg gaggggcgat gaatgcacct aatcagagca
     5401 acgaggacta caaaaagtag cttcttgatg caagacgact ttggaatccc ttagaacatg
     5461 aggataagcc cgacagttac acgagaggac aggccatctc ccaggattca tttggaacga
     5521 tgacctcgca ctcgaaaccg catgtaatca ctacggacga atgaatctct caactacttg
     5581 aaactgctca tattttatgt caattgccac agcgatcagc tgagacagct tgaacgacga
     5641 cctagggtgc cgtccaactg gacagaatca ggaccctgcg ctagactttt ctcacaagga
     5701 acagacttcg tctcttcgaa tgttcctata gaagaaagag aggcacggta tgcgcgggac
     5761 tttggccaca atcatgttgg gactgcttct gaccacgggc tgctccacca ccaatcaggt
     5821 ggcgacgcag gaccagaact acaacgcgcc gctcagcatc tatagcgcga tggacagcac
     5881 ctccctggtc tattccgatc ccatggccgc ctcacaggcg aacgacaatc cttaccgatg
     5941 gctgggcttt gtgctccacc cggtcgggca tctcgtagac tacacggtga atcggccact
     6001 ctacgccatt gcgagacatg cgccgtacct cttcggctac acggccgaag attctatgat
     6061 cgactcgcaa cgtcggtaac ctgctccacc cctcgcctgc cgggatcttt tctcggcggc
     6121 gaggagaagc ctgtcattac tccgcctgcc agtcgcatct ctacagccat tcaggtatgc
     6181 tctattcctc gatcctatat ggcattgacc tggggcatcg attccggcca tgcaccgtcg
     6241 gctctcactg ctgcctgcac tgctcaccat gttcgccctg ctgacagggt gcgaaactcc
     6301 gccgccagcc cgtgcgccgc ttccgccatc ggaaagtgac ctcgacctcc tgaaatccac
     6361 gaaactttgc gatacccgca aggtgttcca agagcaccat cctggcatcg ttccgaaaac
     6421 ccaagcgtgg ggcagtgggc aagagctgcg cttttcggcc gaacagagcc tgtccaaggg
     6481 cgaagagtcg tacttcttcg atgaagacgg gacactggtc gggacgctgt tcatctttcg
     6541 gtccggactc gacttgggtc cgtacaaaac gcttcgctat accctgaccc aactgaaacc
     6601 cagcctggag ttctacctga ccgtcgctca attggcggat cgtcagaata tggcctccag
     6661 caccctctat gacacgggcg atgagaagac caccacccgg tatctcgtcc tgggcgatcg
     6721 aaagaatccg cagcttctgg ctgcgtcctt ttccattgat ccgtacgtga agctcttctc
     6781 tccctaccgg aaagagtttc tcgatcggct gcgggatacg ggacagcaaa gcggtggcca
     6841 acacctcgac acgcagggcg cggaagacaa ggagcctttc gcctcgctcc aacaattcgc
     6901 ccgaggccaa accgcacaac tcgcatactg tggagcgaaa aatcagacca tcgcgctcga
     6961 tgcctaccag aaggcaagcg cttcaggatt caccagccag gtctggcaag cggaactgca
     7021 tcaccggctg ggtgtatcct gggaagctgc cggcgacttg gaaaaggcca agcgcgaact
     7081 gctcgcctca ctggcacaac gtcccaactc cccggaggtc gtcaataatc tgggctatgt
     7141 gtatggcaag ctgggcgaga agcagaacgc gctcgcgtcc ttcgaaaaag ctgtgctgat
     7201 gcgcccgaat tacgccattg cccggttcaa cctggccgag gccattgcgg aggctaaccc
     7261 cagacgcgct atcaatgaat atgagacgta tctggcgctt actgagggca ttccggaaga
     7321 agcggatcgc tcagccctgg cgcaatcacg cgtcaaagcc ctcaaacacc agtaatgaaa
     7381 cgggtgctgt gcggcaaaat cctgcgtgtc acaaaaggaa ttcgaagggg acagaaccga
     7441 atcagcgggg agacttctct gcttcttcct gactggtctt gcattcgatg cacaaggtcg
     7501 tgaccggacg cgccttcagt cgcttatagg gaatatcgcc ctggcaacct tcgcagatcc
     7561 cgtaggtatg cgtggcaagc cgccccaaag cttcatcgat cttcttaagg agtttccgtt
     7621 cccgttcgcg aatgcgaaat gagaaatgct gatccgcttc ggcggaggct tggtcgctca
     7681 catccggaaa cacttccaga ccggtagggt tcgtcagcac cactccggct tcggcaagga
     7741 tagcggcacg ttggccctcg aggtctttct gaatatcagg atacttaacc ccgttcgcct
     7801 tcgcaggttt ctttcgggtg ggagtggatt tcttcgccga agtcttcatt gaatactcgg
     7861 tttactaggc cggtatcgcc gccagaccgc ccaacgagtt ccgactatag cagccgcaaa
     7921 tcggaggggt caatcggttc gtctggggtg ggggcgagga tgaggattat ccgcaactgg
     7981 gcactctcaa aggcagcggg acgtctgcgt cacaaatctc gtcggccttc gatcgatttc
     8041 accagcgtca cctcatccgc atactcgatg tccatcccaa cggggattcc ataggcaatc
     8101 cgcgtgaccc ggacgtggtg aggcttcaag agtcgcgtca ggtagatcgc cgtcgcctca
     8161 ccttcaatcg tgggattggt ggccacgatg acttcttcga cacctccggc tttgacccgc
     8221 tcaagcaatt cctccgcgcg aatatcggat ggcccgacgc catccaatgg cgataagact
     8281 ccgagcaaga cgtggtacaa accccggtac ccaccggcgc gctctatcgc atacagggtg
     8341 ctcggctctt ccaccacgag aatcttgctg cgatcccgct tagaatcccg gcagaactca
     8401 cagagctccc cctcagcgat attgcgacat tggcggcaaa acgagagtcc gtccttcacg
     8461 gcttgaatgg cttcggccaa gcgcaacgca tcttcccgct ccgccttgag caggtggaac
     8521 gccagccgtt gcgcgctttt ctgaccgatt ccgggcaaac gcaccaactc acgaaccaat
     8581 tttgccaaca gtccctgctg atcaatactc atagcgcatc gtcacatcca ggccacacac
     8641 acgagaggcc catagcctcc cgcattcgac acaacacaat ctcgatcgca tcagaacaga
     8701 ccaggaatct tcattccacc cgtgacggct ttcatttcct cagccatcat atcgcgtgat
     8761 ttcttcaagg catcgttacc cgccgcaaca acaagatcct gcaacatatc gacatcacca
     8821 gacttcacga cttccggatc gatcttcacg ctcagcagat ccattgcgcc gttcacggtc
     8881 acagtcacaa tgcccccacc cgcggtcccg gataccgtct tggtcgcagc ctgctcctgc
     8941 accttagcca tttgttcctg catggcctgt gcttgtttca aaatgttgga catattcccg
     9001 aatggacttt tcattcctgt acctcctgtg ccggagttgt cgtgcgaacc tcggccaact
     9061 ctccgccgaa catctccagc gcttgtttaa cgagaggatg cgccttcgcc tgttgtgtca
     9121 ggatgacgcg ctgctcctgc tccttcgcaa cccgtaattg cttcatcgtg ggggtggcct
     9181 ccggatcctg ctccgccact tcgataattc ggacacgcaa ggcagttccg tatattcgtt
     9241 ctcccaacgc cgccagggac tgaagattgt cttccttctc cagcatggct cgcgccaccg
     9301 tcgcctgttt ggtaaagccc aacgtaacca atccaccttc aatcttcact acccggccca
     9361 tttccaaaaa cggcgcaata ttgggatgct gacccgaaac ggcctcttga aactcctccc
     9421 aattaatctc tgccgagggt gaggcggttg ccctgtgaga cgggggttcg ggttttggcg
     9481 acgccgactc gaccggaacc tgcgcggtcg cggaggcctg cgcagatgga atggaaggag
     9541 caggagtctg actggatgga accaccgctt cacgaggacg cgcaacagga gccggcgccg
     9601 gagccgatgc gctcggaaca accggtttgt ctccggcgtc agagctgctt ttcaccgtat
     9661 cacgtttcgt gggtttgggt gacaccacct tctccgatga tgacatcgat gtgcgagttc
     9721 ccgttgcccg atcgacaggc gttgcacttg actgaacatt cactcccggc aaccccgccg
     9781 gggattcagg cgcgcgcagg agacgagtcg cacgaaccgc agccgtttcc agcacaaacc
     9841 ggggatgggc gctgacccgc agatgatcct ctgccgccgc ccacaatcga aataattcct
     9901 ggaggtgctc caccgaaaac tgttctgcgt cccgagccag ttgcgtcagg tcttcatcgg
     9961 tcacttcgat cagtccccgc aactccggac ctgcaggcac tacggcagcg accagcatat
    10021 tccgcacata ctccaccaga tcggcgcagt aggcgcggac gtcgtggcct tgatccaaga
    10081 gtccggcgat gacctgcaag gctttggcgc tgttctggtc gatcacggcc tcaaccatcg
    10141 cccgcacccg ctcctgcgga accgcgccga gcagggtttc aagatcgtga tgccgaatcg
    10201 tcttgccacc gaacgcaatc acttggtcca gcaagctgag gccatcgcgc atgctacctt
    10261 cgctggcacg cgccagggcc atcaagcttc gatcttcgat ggtcaagcca tcctggtccg
    10321 ccacatgccg cagccgctgc acaatctcag cctttgaaat ccggcgaaag ttgtaatgtt
    10381 gacagcgcga gagaatcgtc gcggggatct tgtgaatttc cgtcgtcgcg aagatgaaca
    10441 cgacgtggga cggaggctct tccaaagttt tgagcaaagc gttgaacgcc gagttggaga
    10501 gcatgtgcac ttcatcgatg atgtagacac gatactgtcc tcggaacggg gtgaacttga
    10561 cattctcgcg aatttcccgc acgtcgtcca cgctcgtatt cgacgcgccg tcgatttcga
    10621 cgacatctac cgacgtaccc tgcgtgattt cctgacaatt cgcacaggtg ttacacgggg
    10681 aaccggtcgg accctgctcg cagttgagtg ccttcgccag gatccgcgcg accgtcgttt
    10741 ttccgacgcc acgcgtgccc gaaaagagaa aggcgtgggc gattcgcttg gtggcgatcg
    10801 aattcatcaa cgtctgaacg acgtgtgact gcccgatgac atcatcaaac gttccaggcc
    10861 ggtattttct tgcagagact tggtaatcca atttcgtcag gctctaacta gtagtgacga
    10921 ttgtgtgtcg agtggcagtt cgggacgcca gagcagacat tcttccactt gtggctcagt
    10981 cgtctaaaaa agtggggcca ggtgatcctg cggcacacag aacgaaccgc ttaccgttgc
    11041 ttccttccgg acctggcggg gttcacaggg ttctgttgca cagggcccgg ccccgaggat
    11101 ttcgtgatcc ggcagggatg aaaggcaaag ggcagagcct tgtatgactc ccccttctca
    11161 taccggttcc cgtcatgccc gacgtgacac gactctaatc gatggcggag agggtgggca
    11221 ttcgaagtca cgcatacgcg ctcccactga gggggcctgc cccctcgtgt ctccccacgc
    11281 aggggagtcc ctcccccgcg attccccctt gttcgaatcc caccgcctca gccggcaatg
    11341 gtcattcgac tcgcacgcct tcccaatctt acttcgcact ttcgatggcg gagagggtgg
    11401 gattcgaacc cacggtcccg ttgccgagac gcatgctttc caagcatgtc gattcgtcca
    11461 ctctcgcacc tctccgcgat cggacgactt caaagggtgg catcttatca cgcaggccgg
    11521 tgagcggcaa ggtaagggac gccacgtccc ttacctcaga ctcgatctcg aaacggcacg
    11581 gcggagagac cacgacgggg gttttccatc gcgacgtcgt gcgaggcaag gccgatttgc
    11641 cgccattgac cgatttcgcg cgcattccta tactgcggga ccatttcagc ggaggtacgt
    11701 gatgaaaatt ctggtcggga tcggtgtcgt gatcctgctc tttctgatcc tgatcatcgc
    11761 tttgcctttc ctcatcgatt tgaacaagta tcaggatcgg taccggcctt tgattgagga
    11821 ggcgctcaac cggaaagtcg aactgcagga tatccgtctg accatttggc cacgcatcgg
    11881 ggctcgaatc ggcgggtttg ttgtccagga tgacccggcg ttccgcacgg ggccctttgc
    11941 ctctctcgcg tcgctggatg tgggcgtcaa gctcctgccg ctgctcaaag gcagagtcga
    12001 tgtggaagag atcaccctgc gcgacccggt cattctggtc ctgaaaaatt cgcaggggca
    12061 gctcaacatc tctaccatcg gcgcgaaacc cacggctcct gccgcgccct caaaacccga
    12121 ggccccagga caaccggccg gcagcccgct ccaagcgctg gcgctttttg ccgtcgaccg
    12181 ggtttcgatc gatggcggga agctgacgta tcgtgacgag tccaccgcga agccgacgga
    12241 gtacaccgtc acccagctgg aatttttgct gacgtcggtc catctgggcg atagtcccac
    12301 ggtgcacctg ggagcaaccg tcctgcccta taatcttccc gtgcatctgg atgggacatt
    12361 cggaccactg gttgaaacgc tggatgtgaa atccttctcg ttcaacctcg gccttggcaa
    12421 ggtcgcagtg gggctcaaag gacgcgcagt cggcggcaac ctggatgcca cgattaatgc
    12481 gttgcaaatc gatacggcgg atcttcccat cgcattgccg ctcacaaagc ctgtccagat
    12541 caaggatctg catctcacgc tccatgctct ctacccgatc ccgcctgata ccccgcccgc
    12601 cagccaggta gacctgacgg acttgggcct aaccctggtg atgggtggct cagccatcaa
    12661 catcaagggc acggcaacca aaggcctcgc caatatcacc gcggcgtcgg ccagtatcaa
    12721 ttcctccgac cttccagtgg ccgtcccact agccaaacca gtcgaagtga aagacctgca
    12781 catgaacgtt cgggcgaagt atccgcccaa agaaggcacc gcgccccttg agctcgcaga
    12841 ggtgccgaac ctgggcctga cgatcgcatt gggaagctca cgggtcgagg tcaagggatc
    12901 ggtgctcggc ggactggcga aggtgacggc gacctcaaaa ctcgtgaaca gcgccgattt
    12961 gcctgtggca ttgcccttga aaaagcccgt agagatcaaa gacctccagg cggcggccga
    13021 aatgaagggg caagaggcgc gactcaccaa cctcacgctc caagtcttcg gaggcttcct
    13081 gcgcacgcag ggaacgttag gcctgggaac tcccactccg ccatttaccg gcacggtcgc
    13141 actacaaggg ctacaagtcg gaccggcgct gcaggctgtc ggcacagacc aggtctcgct
    13201 cagcggcacc gccaacgccg aactggcgct cagcgggcgt ggctttactc atccggatct
    13261 cgtgaaagcc ctggcgggaa ccggccgcat gtccgtcaga gagggcaaaa tcgaggggat
    13321 caatttattg caacaggccg cgatgttgct caaagtggtc ggcgtctcgt tggacaatgt
    13381 caaagccacg gcgttctcca ccatcgaaag cgacgtggtg atcaaacaag gactgatcgg
    13441 cctacagcgc gtcttgatcg acagccatga tttccaggcg accggcggcg gcacgatcgg
    13501 cctcgaccaa tcactcgaca tgaaactgaa tctgaatctg tctcaggccc tgagccagaa
    13561 aatcgccgcc ggctctccca ttgccaaggt cgccatgacc aatggacgcc tctcgcttcc
    13621 actggtaatt accggcagca ctcaagcccc ttcgtatgga ctcgacacaa aaatgttcgc
    13681 agggaaagtc caggagcagg tgaaagaaaa ggtcaagggc gcggtgggag atctcctgaa
    13741 ggggtcggcc aagccggagg acctcaagaa gcaaggcaaa gatcttctca agggattgtt
    13801 cgggcggtag gagggggcgg aggaaacatg tcactcgcca atttgattac tcaatacgtg
    13861 gtgcagtacg gatttcgcat catgggggcg attctcgtca ttgtcgccgc ccttttcgtg
    13921 gccaattcgg tgggccgtct cttcgagcgc tggctgaacg aacaggacct cgagccgccc
    13981 ctccgcctgc tgctcctgcg actcctgaaa gccctgctgg tcatcctcgg ggttctcatt
    14041 gcgctggatc aactcggcgt gcaaatcgcg ccgctggtgg caggccttgg cgtcgccggt
    14101 ctcggtgtcg gactggcttt gcaaggggtt ctcagcaatg tggtagcggg gctttcgatt
    14161 atcttcacaa agccctaccg ggtcggcgaa catatctccc tcttgggcgt ccatggagat
    14221 gtcgcgaaga tcgatatctt cacgaccact ctgctgcatc ccgaccattc cagaatcgtc
    14281 attccgaacc gcaaagtcgt gggagaaatt ctgcacaact tcggaaccat ccggcagttg
    14341 gacctctcca ttggagtgtc ctatcagacc gatatcgaga ccacactccg cgtgctgcgc
    14401 gagctcgtct ccagacatcc gaaagtcctt caagatcctg tcccgattat cgggattgcc
    14461 gccttcgccg actcctctat taccctgtcc attcggccct gggtccctgt cgcttccgtc
    14521 ggttcagcac atatcgaact caaccaggcc atcatccagc gattgcgcgc agatggcgtg
    14581 gatattccct ttccgcaacg tgaagtgcgc ctgctgaacc cttcctaatc gaacagcaca
    14641 cgaccctatc atcggcccta tctggtccac tggtgatgcc ccccgcgtgg cttgttgcac
    14701 ctgcgtaaat atgctagtgt accccccctt tccttgacgg acgactgcga ccctggagac
    14761 gacaccatga gcaggcaaac agatctcgat cgcacccgac gttcggaacg cacccccacc
    14821 acgctcgccg agacggagac ggaaaccgcc ctcgccgacg atcccaccgc cggtaataat
    14881 ctcgacaaga tccgcgacat cctcttcggg gcccaggtgc gcgatcacga gcgccgattt
    14941 accaagctcg aaacgcaatt gctcgcagag gccgcccaac tacgtaatga tctcaaagac
    15001 cgtttcgcgt cgctggaaca atacatccgt acagaggtgg aacaagtgac ggggcggctg
    15061 aacgcggagg agcagggtcg cacaaaggcg gtcagtgcgc tgaccgcaga gcttcacacc
    15121 ctcgccgggg ttctccagca aacggccacg cagttgagag aacagagcga acaggcagac
    15181 cgcgatctgc gagaccagct gcagcatcag gccgcctcac tgacgggtga gttcacgcaa
    15241 caacatgcga ccctgtcggc gacactcgat caggccgtgc aacaactttc ccatcagaag
    15301 accgaccgga catcgctggc gaccttgttc caggaactct cgcagaggct ctcgcatgat
    15361 cagcctccac aacaggcata atccaggttt catgcctgcg tggtcccggc ctgccctcct
    15421 cacacagagc cgcagcgttc atcgtcacat gtactccact gtgcccctcc ctcatcgcgc
    15481 tcgacagagg aaccggtatg gcacagccac ctaaccgcag cactccccct gcagaacacg
    15541 actatgctga actacgcagc cttctgctgg cgccggaaca gacgcgtttg gagcagctcc
    15601 aggaacaggt cgagcatatc gacctcaatg cgaagaacct cagccgcgtg ctgccggatg
    15661 cgatcgcgct acgcggagag ggtgaccacc agctcacaca cgtcttaact ccgcacgtat
    15721 cggaagcgtt gggcgcgtcc gtgcggagac agcctcatat gatcgtggat gccatcgctc
    15781 cgatcatgat gccggccatt cgccaggcca tcgccaatgc cttacgcggc atggtccaat
    15841 cgctcaatca aaccatcgaa catagcctgt cgatccaaag tgtgaaatgg agagtggaag
    15901 cgctgcgcac cggcaagccg ttcgcagaaa tcgttcttct gcataccttg cgctatcggg
    15961 ttgaacaggt gtttctcatt cactcacaga ccggactgct gctggcgcat gccgccggcg
    16021 acgcagtggc cgtgcaggac cagaccctgg tgtccggtat gctgtcggcc atccggagct
    16081 tcgtgcagga ttcattcggc gccgacccgg aacaggtgct gaatacgttg caggtcggtg
    16141 agctcaccgt ctggatcgaa caggggccgg cggctattct cgccgcggtg attcgcggca
    16201 ctccaccgga aagccttcgc ctccatcttc aagacacgct cacccgtatt cacgccgaac
    16261 gggcggatgc cttggcccgg ttcaccggag atgccgccac gttcgccagt gtcacaccac
    16321 tattggaaga gtgtttgcgc gcccagttcg aatcacgccg gcgcggcatc gcaccgatga
    16381 catgggtgtt gatcattggc gcgctcctcg tcacgacctg gtgggccctg tcaacctacc
    16441 aggagcgcca acgctggcag agctacctgt ctcgcctggc cacggagcct ggacttgtcg
    16501 tcaccgacgc tgagccggaa ggcggtcgct atcggctcac cggccttcgt gaccccctcg
    16561 cggcggaccc ggctatcctc atgcaagaca gcggcatcga caccagtcga gtggtggcga
    16621 agtggcaacc gtactacgcc ctcgacacgt cgttcattct gaaacgcgcg ggattcgcac
    16681 tctcaccacc accgacggtt cacttggccc tggaaggaag ccatctgact gccggaggca
    16741 cggcttcggc ggattggatc aggcaaagcc gctcagtcgc tcgcgcgata cccggcctcg
    16801 atggctatga cgatcgacgc ctcgtgagtc agtctctcga catgttgcgt cagcaattca
    16861 ccgagacgtc aattttattt gaacagggga gcagcaatct cgtatcgccg gaacaacttc
    16921 aacaagcccg ccgcatcgtc gggatgcttc ggcagctgga ggacctggcc cgccttgcca
    16981 gcgccgccgt gacggtgtcc atcgtcggac aaaccgatgt cgtaggcggg acgagccaga
    17041 atcgacgcct cagtgacggt cgcgccaaag ctgtcctcga tgcgatccac cccgcatcct
    17101 ttcccgcact cacgtttcac acaaggggca tggcccaaga ggctgaattg cagcggtcga
    17161 ccccagccgt cgcccatccg cagcatcggc gagcctcact ggaaatcacc attcagccga
    17221 tgccgtgaga ctctccgcat gattgagaaa aagatctgta tgttgggcgc gtttgccgtg
    17281 gggaaaacca gtctggttcg gcgttttgtc agcagctgct tttccgacca gtaccagaca
    17341 accatcggcg tcacagtcga caaaaaatcg atgacggtcg acgggacatc tctcaccatg
    17401 gtcctgtggg atctttacgg agaagatgaa tttcaaaagc tacgctcgtc ctatctgcgg
    17461 gggtcctcag ggtatctcct tgtccttgac ggcatgcggc gggcgacgct ggacattgcc
    17521 ttgcacgtcc aggaacgggc atccgataca ctcggccccg taccgttcgt cgtcctgatc
    17581 aataagcacg accggagagc ggagtgggat atcactgatc aagaccttgc ccagttggcg
    17641 caacgaggat ggtcagtcgt gatgagcagc gccaaaaccg ggcaaggcgt ggaagaagca
    17701 ttcaccacac tcgctcgcgc catgctcaca ccatcgaact cttcggcagc agccgggagg
    17761 ccccatggct cctgatccgt cactccgcac gccggacttc ctgtgctcgt tattcgagcg
    17821 attggagtac gtcgtcctcg aacatgttgc cggcacgcaa ttccgcatcc tcggccatcc
    17881 atcaccctgg ctgtttcgtc tctatccgga ggctgaagcg agtcttcagc tctccgtcga
    17941 cagtcgcacc cccgtgctgc ataattttct ggctgacgcg gcagaggtgt ggcagaccgg
    18001 cgacgcgcac atcggccgct ctggcttctg gactgaacag accgccttcg acgtcccctg
    18061 gcactttgaa gcattggccc tccgcgaagg aaccagccgt ctgttggtca tacagcacag
    18121 cactgcggcc tatgaacaac aggccgctat gctccagcgg gcgcgcgagc aggcacttca
    18181 acagcatgag caggagcgcg gacaccaacg cacgcaacag gatctgacca tgcaactggt
    18241 ggacatggaa cgggcgcggg acgatgtcgc ggctatcctc caggacctgg ggctggccac
    18301 cctgctcatc gatcaggaag gacatgtgcg ctttgtgagt gcatctgccg ccagtctgtt
    18361 ggagcggccc tcagccagca tcaagcctga tctacggtgg gagaccctcc ttccagtcac
    18421 aaaggccgat cgactgacgg tgcagtcgct tcttgagcat ccgatcccgc aacgggaacg
    18481 ggtttgctgc cacatcgaca cgccggcagg gaggcgcctg tggctcgacc tagagctgca
    18541 cgacgatccg cgggacagac ggagcaagat cgtctttctc catgacatga cggacgtcta
    18601 tcaccttcgg cgtatgctgg aactcaaagc gcactatcac gatttgatcg gcaagagtcg
    18661 cggcatgacc cagatctatg aacagctcca agacctggcg cgcgtggatt cgaccgttct
    18721 gatcgaaggt gagaccggga cgggaaaaga actggtcgcc cgcgccttgc accaagccag
    18781 tgcccgacgc acgggcccct ttatcgcggc taattgcgca ggcctgacgg attccttgct
    18841 cggcagccag ctcttcggtc acaaaaaagg ggcctttacc ggcgcgatcg atgaccagca
    18901 ggggctcttc gaagccgcgc aggggggagt gctgtttctc gatgagatcg gcgacattcc
    18961 ccacacggtg cagaccaatc tgctgcgagt gctgcaggaa aaggaagtga cccggcttgg
    19021 cgagaccaag ccgagaaaaa ttaacgtgcg cgtcctgacg gcgacgcacc acaatctcag
    19081 ccaggacgtg gccaagggaa cctttcgcgc cgatcttctc taccgcattc gggtcgctcg
    19141 cattcacctt cccccgctga gggagcgaaa ggaagatatc ccgctgctgg tgacctcttt
    19201 cctcgctgaa gggcgagcca gcatgggaaa actcatccac cgggcaagcc cggcgaccat
    19261 ggccgcgttg atggactacc agtggcccgg caacgtgcga gaactcaaga gcacgatcga
    19321 atgcgcgatg attcactgca aaggtgactc actcgaagcg acggatcttc cgccggagct
    19381 gcagcgggag ccgacagcgc ttacgcctga acccatcacg actgacgaac gcagccgctt
    19441 cgtttccgcc cttggacagg cccgtggaaa ccgcaccaaa gccgcacgcc ttctcggcat
    19501 gagccgcgcc accttctacc gccgaatgac tgaactcgac ctgccggcca actagccgcc
    19561 tagactgtcc gaccttccgc ggacgctgtc tcacatcgtc tccagcgaga caatgaaaga
    19621 cgatgtgatg tgtctcatcc tatcgccacg gtccctcgca tcgcgccggc tgcatgctga
    19681 tccagcgcaa tggctcatcc tgacgcacat tccgcaagtt caggcacgga gcttgtaaaa
    19741 accaccggac aaaccactgc gtgcaattcc gtgagatcac cgggcaggac gatcagaaag
    19801 gggcatgaga agcaacgatg gataaggtat ttccgttttc aaccaaccgg ccggaccaac
    19861 cgacgccgcg cgagcaagag atactgaatt acatttgggc cggattaacc agccaggaaa
    19921 ttgcggcccg actgcagatc gcacagaaga ccgtggagtc tcatcgcgcc aacttgctga
    19981 gaaagttccg ggctacgaac gccgcgcaac tgctccgcct ggcgttgttg gaagggctgc
    20041 tctccgtccc gtcaacagag cgcgcaccgg atgtaaaacg agtggtgcag agcgcgaagg
    20101 cctcgcgaag actcaccagc atcaaatgaa gagtcccgct ttgtctctac gctcacgtcg
    20161 gcatacagcc tattcgtcca tgcgccggcg tgtcacgatc acccaatgcc gtctcatctt
    20221 gacacatgtg tactgtgcgg ctccgcgata atctgtgcgg acgcttcttc gcaacgggtc
    20281 ctggaccgtc tggtcggcgg aaacatcatg atggcggcag attgccgggg acagtccgtg
    20341 agcgcgggag gcggcagatc tcatctatgc gactcggcgg gagccagggc catgcggaag
    20401 aatgggcggg agaaaagttg atctcctacg cagaatcaat tatacaacgc aaaatgtctt
    20461 tcactcatca ggcaaccagc acaccgttgc cattcgcaag ccccaggagg atggaatgat
    20521 gcgacgctat gcatgtttct ttcttgcgct gctcatcgta gcgcttacgg cgtacggcgt
    20581 gaatgccgaa tcaccgacgc ccacggatac cactgccgca gcgccgacac tggactcggt
    20641 cacgctcaaa gacggcaccg tcatctatgg acgtgttctc ggcatgatcg ccgatgagtt
    20701 gcacattaag actggatttg gcccgaccgc cggagacgac atcgtgaaga tcatgtggcc
    20761 gaacgtggcc aaactggtcg tgaaccgccc cattccgttc accctcaaag agggcaccac
    20821 ggtggtcggc acggcacagc ccggcgaacc gggcacgctc accttgcaag ccgcgccgat
    20881 gggaacgcca atggcgattc cgctggatgc ggttgtaggc atgaatcagc ctgccgtgat
    20941 ttatacgggc gcgcttcaag ccgggttttc ccaaaccacc ggcaacagcc atttgcgaaa
    21001 cggcagcctg ctcggcgagt tgtctgcccg cagcgaatca ctgcggctga cgatcctcgg
    21061 ccgttacatc tacggagaca atagcgggaa cctcatcgtg cggaacagcc gcggcaccat
    21121 caagcttgac ttctttctga cgaagcggtt gtattggttc gcgtccgcct atttcgagca
    21181 ggacaccttt caggacttga aactgcgcac cgcgctcgcg accggtcccg gctatcaact
    21241 cctcgatcgc ggtgacctga caggtttctt caaagacatg acgctctggg cagaagccgg
    21301 cgccgcgtac ttcaatgaag atttcaagct cgccgacgac aagtccagca cccgtggccg
    21361 atgggccgtc aaatggaatt ggccgctgtg gggcggcgat caggtcagcc tgtaccactt
    21421 ccaggagggc ttccaatcgc tggccaattc caaagatctc tatctcacgg ctgataccgg
    21481 gttgcgattc aaagtgtggg gcgggctggt cagcggattc caatggacca tgcggtacaa
    21541 caagaatccg ccgccaggtg tgtcggatac cgataacctc tacctgatca cgctgggata
    21601 cagcttcgac accagtcgaa aacaataaga tgcgggacgg tttcgggatt gattcgactc
    21661 acataaggag ggtgcacaat gggcatgatg agtgagttca aagaatttgc cgtaaaaggg
    21721 aatgtgctcg acatggccgt gggggtcatc atcggagggg cattcgggaa aatcgtgtca
    21781 tccgtggtca gtgatattct catgccgccc atcggtctcc tcatgggaca catggacttc
    21841 tccagtctct ttattccgtt gagtgaagag gccaaaggca aatccctcgc cgcggccaaa
    21901 gccgccggtg ccgccacgat taattatggc gtcttcctgc agactctgct cgattttacg
    21961 attctggcct tcgtgatctt tttagtggtc aaacaaatga atcgctttaa gaaggccacg
    22021 cctcccggcc ccccaccggc gccaccaaag gaagaagtct tgttgaccga gatccgtgat
    22081 ctcctgaaga atcaacgtca ctaaccatgg ggcgttcgcc cagtgctgct gggaacattc
    22141 cggcagtctg actgtgttgt aaggaggatt gcatgaaaca cgtttgcgtc gttcccgtgc
    22201 tgctgagcgc tgcattgctc gcagcctgca catctacacc ggcacccaat gactcagcga
    22261 gtcgagcgct gaatgagatg aaggccgtcg ccaggcccat cccgcctaag ccggatccgc
    22321 gcgatctccg gatcgcagaa ctggaacgcc agaaaaccga tctcgaaggc gagttggatc
    22381 gcctgcgctc atcaagcgcc gccgactcag atcggtccaa ggcgcggatc accgaactgg
    22441 agaaccaact gaatcagcgc gaccgcgaac tggctgggct tcgcaacgcc gcgggcgaca
    22501 aggatcgatt ggccggccaa ttgtccgacg cggaccgacg cttgtccgcc aaggatcaag
    22561 aactggcggc gttgaagcag agcgccgctg acaaggaacg gctggccgcc gagttggccg
    22621 ccttgcaagg gttgctcgcg acgaaggacc aggagcttgc cgggctcaaa cataatgccg
    22681 gggatcgtga tcgtctttcg tccgaattgg cacaggcgaa acagcacatt gccgacctgg
    22741 agcgtcagct cagtgggaag gatcaggaac tggggacgct gaagggtgcg gccggtgatc
    22801 gcgataggct cgtggcggat ctcgccgccg ccaagcagcg agcatcggac ctcgaaaatg
    22861 aaatcggacg gcgagaccat gagatggccg gtctccgtgg agcactcgat caacaaaaga
    22921 ccagtctcgc cgaagcgaaa aacgatttgt cgaaactcct ccaggcagag gtatccaagg
    22981 gcaacgtgac gatgaaacaa ctgggggatc agcttaccct cggtttagcc acgaccttgc
    23041 tgttcgactc cggagaggcc accctgaaac caggcggagc ggatgtcttg aatcgtatcg
    23101 gcagcgtact caagaactat ccggatcgct ccatccatgt ggcaggccac acggacaacg
    23161 tgccaatcaa gggacgattg gcaaagcggt tcccgaccaa cgccgagctc tcccaggcgc
    23221 gagccgagag cgcacgccaa gcgttgacgg aaggcggtat ggccgccgag aagattagcg
    23281 ccaaaggtca tgccgacagc cgacccatcg caagcaattc tacggccgag ggacggcaga
    23341 aaaaccggcg tgtcgaaatc gtggtcggac agtagtcctc tctcattgcg cgttgtacgc
    23401 cttgcgtcca tcccaagaac acgctagagt acccggcgtg ttcttggggc ctgcatggtt
    23461 gtcacccgat ttgtcaggca cgcagtgcgc acaccggctg atcagcatca accagatcag
    23521 cctccgtcgt ctgggcttgc tgcccatctg aacagtcctg tgacgactat cgccccctcc
    23581 gctatgttcg gcagtcgttc cgatgaccag ggagagatcg tatgagacga gctgtgtggg
    23641 cgctcatggt tctcggtgcc cttgcttcgg cctgtgagac cgtgcctcag ccacagccgg
    23701 tcgcagcccc accccaggta ggcgatgcca aactcctagc ccttcaacag gagcgcgaac
    23761 agctcctgac gactctcggg gaattccacg atcgtattcg cgatcttgaa agcaaattgg
    23821 gggatcgcca gaaccaaccg gccgcggctt cgtatgacca gttgctgaac gccaaagatg
    23881 cggaattagc agagctgcgc cgactctctc ctgaacgcga caaactcacc agtcagttgg
    23941 ccatggccaa taatgaatta ctgcaagccc gtcagcgcat cggaagcttg gaacagcaac
    24001 tggcaacacg agaaaaagat ctggttgcgc ttcaaggccg ctccacagca atttccgatc
    24061 tcgaagtcgc ccgccgccgc atcatggatc tggaagccca ggtcgctcgg caggatcacg
    24121 atctccgcac gattaagtct gaaaatgccg aacgggacag tctcgccgcc caattacaaa
    24181 cagccacggc aacgattgat tccctcaaag cgcgcatcag tgcgctcgat cagcagctga
    24241 gagaccgaga gcaagccttc gaaacggttc gctcgcgcct catggaacgg gacaagctgg
    24301 tcccgcaata taatgcgatg atcgcagaga tttaccaggc ccggcaccgg atcgctgcac
    24361 ttgaacaacg cctgaatgaa aagagtcggg atctttcgcc acgccaaaag ggtgcccaag
    24421 cggcgacggc cccacgggaa acgggggcga cttccaacaa aaacgccgga tcctcggaga
    24481 aggatgccgg ccgcacagga agcgcgaccc caacaagtcc gagccagtcc acttcgcgcg
    24541 aggccagctt tgcagccatg aaggaggaga tcctcaaggt tctgccgagc gatggcgggc
    24601 agaaggcgat cacggtgaag caggatggaa gccgattgac gatcgcgctg tccagcaatt
    24661 ggctgtttgc ctccggcgaa gccgccttga cctctgaagg catgacgatg ctcaaacgca
    24721 tcggcactgt gctgggaccg ctttcagaca agtttgtaca agtggccggt cacacggaca
    24781 atcaggcgct gagcaaaacc cttcagaaaa ccttttcgga taataaggcg ctgtcgtggg
    24841 ctcgggcgga aaatgcgcgc agggcattgg tcaccggcgg gatgtctccg gagcgaacca
    24901 aagcggtcgg tttggccgac tccagaccta tcgcttccaa cgcaaccgaa cagggtcgcc
    24961 agaaaaaccg ccgcctcgaa ttggtcattg tgcaaggacc taccgttgca tcgacgacgg
    25021 ggaaaacaac tgaggagcga gatcgtctcg ccgcacttgc tccctcatac tagagccgac
    25081 cattacgact cggcttacaa ctggcaacta cccgattgca ccgtggcgac agactccccg
    25141 gcaacagtgg ccagaatctt gtagtgtccc agtcccagtt ccttgccata cgcttctccg
    25201 actaacacat cctgcacatg ctggtggtcc gaggcccgga agtacgatcg cccttctttc
    25261 aggccatcaa actcatgtcc ttccaatacc ttgatgagcg cggacgcttc agttgtgcct
    25321 gcccgctgaa tcgcttccag aatctgcgtc atggccgcat accccagata acaacgcgat
    25381 gtcggagtat gattgtactt ctccacgact ccctggataa atcgtttgga tccgtcagta
    25441 ttcactttgg ggtcccagat caggccccag atcccggcgt tatttgcata gccgagggga
    25501 cggccaatct gctcgccggc aatcatcccg ccgacaccga ttttatcctt ggctaattcc
    25561 aacttggtgt acgctttaag ggcatggaca agatcccatc catagaggtt caacactacg
    25621 agcgtcgggc gtttgtcctt ggccgcgctg agtgccgagg tgaaatccgt cgaaccgaag
    25681 ggcatcagtg tctcacccac gaattccact ccatgagtaa gaccagtcga ggccatggcc
    25741 tgagccatcg ctttgccatc caaagagctc gtcgtaatca tgtaccaacg agtgccatag
    25801 gctttcacca actgcgggac aacggcctgc gccagcatgg acgcattggg cataaacacg
    25861 aaggtgtggg aattacacgc ggcccctgtc aactcaggca aatgcgcgcc cgtgaccatg
    25921 aacaacttgt tcgccttctt cgcgtactca gacacaacaa gggcgcactc agcgttgaat
    25981 gtccccatca agacatcgac acgatcgtcc tgcacgaatt tcgtcgccgc cttgagcgcg
    26041 gcatcagggc tggaggcatc atccgcctcg atgatcgtca ccggcctccc caatacccca
    26101 ccgcgcttgt tgaagagatc caccgcgacg ttcgccccat gtacatcgtg gatcgaagac
    26161 gtcttatagg ggctcgatag cgggtccaga ataccgattt taatcggctc tgccgccaac
    26221 gccgctgatc gtagaagagg cacattgagt atgcgctcgg cgagatcgcc caaaagcagc
    26281 acgccaccac tcatagcgga tatttgcaga aagcgtcgtc gcgagatatc tctcatcgtc
    26341 ctatccctca ttgttgtctt ggcatgttcc cggcccagtt aatgtgacct acttacacga
    26401 cctcctcgac actgctcgac cttcatagca gagtcgccgc tgttttcaca gctcgaagtt
    26461 gggaatgcta ttttgcagga ccggcagaca ttggacgcga actgcacgcc cattacgaga
    26521 aggctggtga cgcaggatag cgacgccatc gcggagaggt cacggactat tgaacgcggc
    26581 aatactgcac caggatacat gcctagctgc taactgtact ggtcttcggt atacacctgc
    26641 ttgattgacc agaagaccgc ctcctttaat ttcttaatct ggggatcggg aggatctttt
    26701 cggatttttt gcagcttccg gtcgagggca aataacggtt caaccgaccg tcgatccggc
    26761 acccgcccga gagcccacgc aacctccgtt tgcaccacaa tgtcgatgcc gggcgcatca
    26821 agcatctcca gtaaaggcgt caccactctc gagtcactat gaatgttgcc tagatcacca
    26881 agacctttag ccgcagcggc ttgcaactcg ggttgcttgg aatgcttcaa aatgtcgatc
    26941 agcacaggaa tcccttcctt ccccatgttc cccaatgcga ccgcggcttg cttggcgaga
    27001 tctttgtctt ttaaagcctc ggtgaggtgg ggtaacgcct catcagcaac catctctccc
    27061 aacaaggaaa taatcttgag cttccgaatt tggctggttt gcggactatc gagtaattta
    27121 agcagccgat caggctggcc ccactctgcg gtcaggagaa gggggaactc atatgtctcc
    27181 agcgcgtcat ggagcgcatt catggcatac agccccgggt ccggtgcctg agccgattgg
    27241 gcagctccga ccgcatcttc aaattctgtc cgaacctcct tctgccattt gaccttcata
    27301 ccacccaacg cgtacgtaat ctgacatgcc ggacctttcc acaagcggga cggcgcatca
    27361 ggcaaatcat tgtctccccc cgcggatgcc gtgccctccc aggtcttctt ttgctcgcat
    27421 ttgacccgca cgacgacatc gtgggacacg tgtggatccg agactgttgt ataaccgagc
    27481 tcgcccatgc gcttcatggc cacgtccaag attccgtcgg cattgccagc gcctttgtct
    27541 gttagggcca atgcctcaat caagacggtt tgaatctttc ctaattcttg cttttgtgcg
    27601 tcggtaaaat agtctcgata ggcctcgctc gaaagcggga agccgcacag cagaatgaga
    27661 cacccaagtg cagctgtcac agactggcgt ttcatacggt gtcccccttt atttcgaaga
    27721 acgttcggat ggcgatagtg aatcagacct gtgtgtccat tataccgttc ctcatgcagg
    27781 gcaataccag gagaaggagc ctcgcagcac gagagagaca accgcctgat ggaggccttc
    27841 actccataca attttctcta cggccgagtt cccctcagaa tcacaaatca gcacacgggc
    27901 cccacctgcg tatgggttga caggcagaca cgatcgacgg tacaaaatgt cattattcat
    27961 cgtgccttta cacatgtata tccatttctt acgcaccatc agtgagcagc ctagtactca
    28021 gcgtcgcgcg agagtggcgg aactggcaga cgcgcgagac ttaggatctc gtgggtaacc
    28081 gtggggg
//
LOCUS       DAOQEW010000026        27688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_393_length_27688_cov_8.655267, whole genome shotgun sequence.
ACCESSION   DAOQEW010000026 DAOQEW010000000
VERSION     DAOQEW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_27688_cov_8.655267"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PLS35_04810"
     CDS             complement(<1..1250)
                     /locus_tag="PLS35_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18007.1"
                     /translation="MDIKTQLPKVGIGRRCGAIEANLGGYRSLMSDDEYEELTALAKS
                     LQGIKICHVNSTAAGGGVAELLARYIPMLQALGVQADWRLIHGEPEFFAITKAFHNAL
                     QGGHYGLGTTEQDAYLKVNERSAQALGNDYDLYIVHDPQPAAIRHFAGSRDAKWIWRC
                     HIDSSAPDKEVSRFLRPLLEEYEAVVFTMSQFVLPQLAARRVAFIAPAIDPLATKNME
                     LPIDLCRRVIADSGVDPARPLLLQVSRFDPWKDPLGVVRAYRLVKEEIPEVQLVLIGA
                     MAGDDPEGWEILDRVEEEAANDPDLFVFTNLAGVGSMEVNAFQHGCDVVIQKSLREGF
                     GLVVSEALWKEKPVVAGNTGGIPMQFPEPFQRNLVDSVEACAERVRHLLHHPGARAEF
                     GRAGREHVRRHFLMPRLVRDELRL"
     gene            complement(1280..1768)
                     /locus_tag="PLS35_04815"
     CDS             complement(1280..1768)
                     /locus_tag="PLS35_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18008.1"
                     /translation="MVYGMRTALKVGFSFGLTSGVITTLGLMVGLHAGTHSMPAVLGG
                     ILTIALADAMSDALGIHVAEESKNHGNTHDIWASTAATFVAKFVIATTFAVPVLLMPL
                     DLAIVASLLWGLTLLALLSYALARAQRVPPWKVIGEHVIIGLCVVAMTHYVGEWVRLT
                     FG"
     gene            complement(1814..2461)
                     /locus_tag="PLS35_04820"
     CDS             complement(1814..2461)
                     /locus_tag="PLS35_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNI18009.1"
                     /translation="MLRSRVTGWVGLVLAVYTASGCVLPRSEDSAVRTFVLMREETDR
                     QAAPAGAPAGMHGVLVVAVPQAAPGFEQPRIAYLRRPSEVSYYANHVWADSPSRMVLP
                     LLVRSLDTTGLWRTVVPMPTGIKGDYQLDLSGLVVQQEFLQQPSRSRVQVRAHLVDVK
                     AQRVLGARNFEALEPAPSEDAYGGVLAADRAVATVLATMNEWIASCLRGSGRETC"
     gene            complement(2472..3434)
                     /locus_tag="PLS35_04825"
     CDS             complement(2472..3434)
                     /locus_tag="PLS35_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNI18010.1"
                     /translation="MEPKVNYVAVGAFVLLLGATVLGAVLWLGKTDYRGIYDRYYVYT
                     RESVAGLSVDSTVKYRGVDVGRVKEVILNPDNSEEVRVTLDIVGGTPVKTDTQAVLVT
                     QGLTGLVTLNLTGGSKDAPPLTPVTGQVYPVIQSVPSLYGRLDGTLAKLLSDQGLSNL
                     VANVNGLAQNASLFLDEQNRAQFRQILKDLGEVTKVLAARSSQVDRGAQHAAEAAERT
                     ARLTEHLGVQLPALLDRMGKSAAALQHMTAEVTRTSREVGDMVGTSKPGIEQFSRQTL
                     ADAGLLVSELRQLTATLTRVAQQVERQPNVLVLGRQGQPKGPGE"
     gene            complement(3445..>4206)
                     /locus_tag="PLS35_04830"
     CDS             complement(3445..>4206)
                     /locus_tag="PLS35_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI18011.1"
                     /translation="HVSTRFGPAIVHQDVSLIVRKGEVFAIAGGNGCGKSTLLREIVG
                     LLTPTAGRISLLGADSRDLQETDGGSLYRRFGVMFQHGALFSSMTLAENVAVPLREHT
                     SLSARLIRDIVALKIALVGLPPDSGVKFPRELSGGMRRRAALARAIVMDPELLFLDEP
                     TAGLDPMIAAGFDDLVLDLKRLLGLTVVMVTHDLDSLWRIADRVVVLGEGHVLGMGTM
                     EQLAASEDPAVRNYFHGPRGRAAQLNHSGGESHRD"
     assembly_gap    4207..4306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4643..5782)
                     /locus_tag="PLS35_04835"
     CDS             complement(4643..5782)
                     /locus_tag="PLS35_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI18012.1"
                     /translation="MNRPYTMAPRGQSAAVTFLDGRTLRCLGSWTMSDVGPAERALRV
                     MDWPADGSLQFEGSGIHALDTGGAVVLSRAVRDAQRRGCDVTVEGLRSEHAQLMNLVS
                     VHWDAMMAHVAARPKGTARLQSVIRNLLDRFLRGLGFIGESAMALLRVGSSPTHLRWR
                     SALNSLRLDGVNALPITGLLTFLIGVVIAYQGAEQLRKFGTNIFIVDLVGISLLREIA
                     PLIAAILIAGRSGSAYAAHIGTMKVTEELDALRTFGLSPTELLVLPRMLALIVALPLL
                     TVYADVLGVFGGMLIAANQLNVSFTAFLARFEEAVALRHFLIGVGKAPFFAVIIALVG
                     CYQGFQIRGGVDDVGRHTTMSVVQSIFLVIIFDALCSIILNWWNL"
     gene            complement(5791..6972)
                     /locus_tag="PLS35_04840"
     CDS             complement(5791..6972)
                     /locus_tag="PLS35_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNI18013.1"
                     /translation="MKMIGALTRMVVAMAVIFGAAPVSAEKSDGRSPLGERAVLRAGQ
                     VVQGDYFAFGPQVEISGIVNGDLYAAGGEVIVDGVVNGDVIVAGAKVVLSGTISQDAR
                     VAGAHVTVSGTIGRNATLAGADVHLTEKATVRENVIAGGGSVQLSGAIGRDARVGAWH
                     ATFSNEINRDVIVAAESVRLTSKASVGGRLRYWGEVAPSIDEEAVVHGPITRRPLPEG
                     WSTERARQGLVGMRVFAAIASFLSTLLLGLIMLRVWPQLVRQVTGAIRERPAPSVGWG
                     LAALIVTPLVAISFVVTLVVLPAGVMLLALYGVAAYLARIFAITYAGQLFVRRQDDSS
                     SLARPFVVGLLVYSLLTLVPVIGGLVTLFTVMAGFGGLLVAVKQRLAVRQEQDLVQDT
                     A"
     gene            complement(6969..7397)
                     /locus_tag="PLS35_04845"
     CDS             complement(6969..7397)
                     /locus_tag="PLS35_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18014.1"
                     /translation="MHPIHPMVVHFPIALLLASLLFDLVALRWRGEEFRATSLSLLAL
                     GVLAALVALLTGHLAEEAVEHSGTIPKQAIETHEELAFVVFWLFAGLLGVKMLSYWGW
                     MRERSLLVLFLGVGGSVLLLVASYYGGELVYRYGAGVLPR"
     gene            complement(7715..9403)
                     /locus_tag="PLS35_04850"
     CDS             complement(7715..9403)
                     /locus_tag="PLS35_04850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNI18015.1"
                     /translation="MGSVGHEQEAPLLSWCEADTRRYVQMLVLALVSGWAYFANLHLS
                     LLEGSEGLYAGIAGEMARRGEFFDLTYQGIPYFNKPPLFFWLLNLSTSIWGDHEIALR
                     LPGSLAAVGTILLTYVLGTQLMSSRAGFWAALVVATSHVFLWYGRRVLFDSTLTFLVT
                     LALFAWIQVQILGRSSRWYLVTFLSMALGAMLKEMHGFFLPLLIMALYAVIQRDARMV
                     RDRFFWIGLAGAIALLAWYSQLLSVGYQHHFLLGSAIKSVWNSGLSGAAGTSKDGHPL
                     YWYLGMMWADFFPWCALLPSALLLLWQQRPLRAHQTELVLLVWVLGLFTAFSLATLKR
                     EPYLTTVVPGIGLMIGYFYHRSSQSSDGSRTMSPVLRLFLVVLALVFIGGMFFGAAPL
                     RRRWLVSAPVVSPLFIVIMVSLGGALLYAAITSRLQLALRMVGVLAVAYVVLVVNYVF
                     PAIDEAASPRKATEYIKSVAKEAGPPMFLFIPGWPKNEDAVYYLKRDTVVRDLPDEDA
                     VHHAVRTQGPIRVITEESHMVTLQKQAGLVVETLQEFLQPGRKHLYLLSIQPKG"
     gene            complement(9619..12894)
                     /locus_tag="PLS35_04855"
     CDS             complement(9619..12894)
                     /locus_tag="PLS35_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI18016.1"
                     /translation="MTTPRLGASGRLAALFVGSKLTPLIILASLLLGLFAVLLTPREE
                     EPQIVVPMADVWLPFPGASAKVVEEQVTKPIERKLSEIKTVEYLYSISRPGGALIIVR
                     FYVGEPMEQSLVDLYDKLMSNQDLLPPGAAPFQVKPRDVNDVPIVTLTLSSERYTDFQ
                     LRQLGDQVLEDAKKVPGTASGFVVGGRGRELRVQIDPTRLKAYGLTPLRVASVIQGEN
                     LALPAGRFESRNESFLVETGRFIRSKDDLDGVVVGVNEQRPVYLRQVAEVVDGPSETN
                     QYVWFGEGARSSSSSSGETPAVTVAIAKQAGTNAVTVAQGVIRKVEEMKGRLIPADVQ
                     VTVTRDYGETADEKANELLWHLLVAVVAVVVFLGLTLGFRPAFVVSIAIPLTLALTLF
                     ISMLIGYSINRVTLFALIFSIGILVDDAIVVVENTYRHLTLRLLPHREASLFAVDEVG
                     NPTILATMTVIAALLPMAFVSGLMGPYMRPIPVNASIAMFVSLLVAFIVIPWFCQTCY
                     RPGVHMAGVDHDSFEEGRSYRLYRRLLAPVLSHPVMAYLVIGVIGLLLAGSVLLFWTR
                     DVVVKMLPFDNKSELQLVIDMPEGTTLEETARATKALTQYVRTIPEMRDYQAYVGTAS
                     PFNFNGLVRHYYLREQPHEADIQINLVAKDRRQAQSHDIAQRIRPPVQDIGRLYGANV
                     KVVEVPPGPPVQSVLVAEIYGPEYDRQIEIARDVRRMFETTAGVVDVDDYLEAEQVKY
                     VFSVDRAKAALAGIPSEEIVRTLRLALEGADVGLVHIPKERRPVQVKLRLPIIERTGL
                     EHLGEIALRTTAGHMVPLSELIRVEQTVREQAIYHKNQKPVVYVIADVGGTGPDKGES
                     PVYGVMGIGKKLEQYRLPEGYRLEQQYAVQPWSEQRFAMKWDGEWHITYETFRDMGLA
                     FAAALVLIYLLIVAQFQSFLTPVVIMAPIPLTLIGILPGHWLTGSYFTATSMIGFIAL
                     AGIIVRNSILLVDFIQHQEAEGIALLEAVIRAGAIRTRPILLTAAALMVGAFVIILDP
                     IFQGLAVSLLFGVGASTVLTLIVIPVLYYLWIGERRPVSACPLPQADNPAAPRHAVGN
                     QPG"
     gene            complement(12963..14009)
                     /locus_tag="PLS35_04860"
     CDS             complement(12963..14009)
                     /locus_tag="PLS35_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18017.1"
                     /translation="MLALLLAAGCGPSQEPGKPGNMTPGTAGQEPIQVPVIEVAASQV
                     PISVEVTGQVTAVYQATLASRIQGMIDNLLVREGSVVRQGQTLVVLDNRDVQAELSRV
                     TAELDNAKAHQDRMEELYRKDAVSKQELENATRALRVAEAGRKAVLAQLSYTIVKAPF
                     DGVITEKKVERGELASPGQPLLKMEDPGRLRLEATVAESDVRVVSVGSLLPVVIDALG
                     ANPLKGTVSQILPAGDPQTHTFTIKVDLPQAGGLKSGMFGRLKLEKGMSTTLLLPKSA
                     LIERGELASLFVVGGDQTARLRWVRIGRTLEQGVEVLSGVDAGERVIADGSKGRDGAL
                     VQDMTAVALPPQAR"
     gene            complement(14038..14226)
                     /locus_tag="PLS35_04865"
     CDS             complement(14038..14226)
                     /locus_tag="PLS35_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNI18018.1"
                     /translation="MKLNEMLRLIAGVFVLIAVVLGATVHPYYNYFAAFVAANLIQSS
                     FTGWCPMMALLRRLGVHE"
     gene            complement(14345..15298)
                     /locus_tag="PLS35_04870"
     CDS             complement(14345..15298)
                     /locus_tag="PLS35_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18019.1"
                     /translation="MVTPERTAQAEWSAIAEQKTSYTTDAFQFSSARRLRLTEDPSLP
                     TVVSTDKPQDVIWEPALEVIHSTPTMQGKNELSVKAQGAIYTNNPIFNHSDFRIQDRF
                     SLDQATSVLVRYRYIPNLFLGPNFERRTGTRSVQEERVSSHHWRAEVERRLSDTVTAT
                     LITRFGIRRYNDVFAERDTNLWTVGSRVDYRAKEWLTLTLSYLYERGLADGHKETQFA
                     DDVSYYLHMVSAGTEFRLHKEWDLGLTYIHRRKTFTSGIAGDSHVDRFDATHQGIAEL
                     RYHISTAATAMLSFQYGKRTSTNVLRDFQDSIVSIGGQYRF"
     gene            complement(15500..16297)
                     /locus_tag="PLS35_04875"
     CDS             complement(15500..16297)
                     /locus_tag="PLS35_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNI18020.1"
                     /translation="MIEQALRAVVSGVPIGLQLGATGTGGAILGVPLMVYIAGMSLQQ
                     AAAMSLMIVGASSLVGAWEYGRQGMVKPKAAAAFSWTGMIGSWGGAFVHHMIRQEILL
                     VLFGLLLLLVRTVMARQRELAAEHGADESCATLFPRTCWMKVGGIGLMVGTLNGLFGV
                     GGGFMVVPALILVLGFPARAAIGTSLMIIFMISIGGVVGHMQFGHVEWSLLGYVLLGS
                     VTGMVLGVRADTWVSPVVMRRVTASITISIAGILIVVNLAKLAGWLG"
     gene            complement(16318..17565)
                     /locus_tag="PLS35_04880"
     CDS             complement(16318..17565)
                     /locus_tag="PLS35_04880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNI18021.1"
                     /translation="MARVVIIGASIGGLPAAYEARELLAKKHKVTVISNVESFHFVPS
                     NPWVAVGWRTRKDISFALGPVLEKKGVEYIHATADRIEPEQNRVITSKGEVPYDYLII
                     ATGPKLNFGAVPGLGPSGYTQSVCNVDHAEQAWGTYQAFLKDPGPIVVGAAQGASCFG
                     PAYEMAFILDADLRKKKLRKKVPIYFVTPEPYIGHMGLAGVGASRRLMEDEFAEHAMK
                     PMCNTSIKEVQPGKMVLEGGQEIPFRYSMIIPPFAGVDAVAGTPGLCNPKGFVNIDAY
                     QANPKYKNIYSVGVCVAIPPVEQTPIPTGAPKTGYMIESMVSAAVHNIKADIDNDTKR
                     ETATWNAICLADMGDTGVAFVALPQMAPRNVTWAKKGKWVHLAKIALEKYFLRKMKKG
                     VSEPYYEKVILKAMGIAKLEEPA"
     gene            17884..18603
                     /locus_tag="PLS35_04885"
     CDS             17884..18603
                     /locus_tag="PLS35_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HNI18022.1"
                     /translation="MYETHLTWLRRILAGKGLIIALIVGFLAVESIVAACFLSLMHFK
                     TSNNWATRNEQVLIELERVRSIVSGAETHQRGYLITGSDNYLAPYREALDMLQEQIRR
                     IGSLTRDNNLQQDRVAYLATQVDQRSDEMEQAIALRRTKGLPGAKSIVTQNQLNRTMD
                     TIHDITEQIRDEEARVLARNRADSEAWALTTGSLALVFFLLNAVVFALCGVVMKLALS
                     SHAQTERLVDALRPSTTPATR"
     gene            complement(18687..19160)
                     /locus_tag="PLS35_04890"
     CDS             complement(18687..19160)
                     /locus_tag="PLS35_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18023.1"
                     /translation="MFALVWIHLLAAVVWVGGMVFLSVVLVPVLKRDGGFARHAALFR
                     TAAYRFRALVWGAMGILVATGSVLAAGRAIPLSDPSQWPSIFMTKIGLVAFLFTLTLL
                     HDLVVGPQVRRILGTAEAARSAKDRLLVRYSVLVPRVSLLVALLVLLAAVVLART"
     gene            complement(19234..19779)
                     /locus_tag="PLS35_04895"
     CDS             complement(19234..19779)
                     /locus_tag="PLS35_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18024.1"
                     /translation="MASFRIEISGGRALVDITLYLPDDSSLPFPIGEHSPQQAFSHRI
                     EETGSGLHHVVTFTGLPEHIDLALQAIGESVRIPGAWAAVGGQRMSSLVALWNRLDCY
                     RQSLSCSDRVSYCRNKAASVRVGLGCGLAFCSAPCQFLCPLCSLKDTPLAQSIVHPET
                     QVLVVLGEIDWCPNLARPADR"
     gene            complement(19880..20581)
                     /locus_tag="PLS35_04900"
     CDS             complement(19880..20581)
                     /locus_tag="PLS35_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18025.1"
                     /translation="MKRVRKVVQNIGMWGAGVAIAAVLGVAGVQPASAEEGTGNMVFF
                     KGGFMNLNQDRSGQIFTDTAGIAGANGGNAGWYAGAGLDLVMSKDVWGGMDKTWVLGE
                     IGLQFNSINSSRVNNAAGSIATRTLTDTLATDPQKVQLTMVTIDVAPKIKFMEGSAFR
                     PWVIPVGLDIHVISPPSNRTQYLDLGVQFGAGFEYAVWKAFKVGVDARYHLTARLTNT
                     NNDYFQVGPYVGISF"
     gene            complement(20824..21372)
                     /locus_tag="PLS35_04905"
     CDS             complement(20824..21372)
                     /locus_tag="PLS35_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI18026.1"
                     /translation="MVPQGSRRTSLLVDPLALLKREHHMILERLAMIETAMSPCCSGS
                     GAVKQTNRDTLRDLLEFFTGPVDVHFKREEMLVDNLRRTLGRNREEKEQFQSFVEEHQ
                     ALKDDATAVMQQLARKRSDGQDGVEPKACGGLRALTDELHRLIRRYREQIACEERLLF
                     ALAEMRLTADQRRRISQRMLQV"
     gene            complement(21682..22392)
                     /locus_tag="PLS35_04910"
     CDS             complement(21682..22392)
                     /locus_tag="PLS35_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNI18027.1"
                     /translation="MKRKPCLIEHCDTCGLREKVVLCDISGSDLQEFQKIKRTLEYAP
                     HQTVFYEGHLCLGLYLLCSGKVKLTRSSTRGRRQIVRILGPGELIEKHVFGDRALHEV
                     TCETLEPSQVCVIDKERYLAVIHRNPQLAIKLIQLLSNEVGVNMDHLDQFTFKTARER
                     LAGLLLELGDRFGKKKDDQVRVGLTLKREEVAEMAGITVETTIRLLGVFRDEGVLTID
                     GRTITLLNPDRLSRIAER"
     gene            complement(22862..23821)
                     /locus_tag="PLS35_04915"
     CDS             complement(22862..23821)
                     /locus_tag="PLS35_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI18028.1"
                     /translation="MKRLQKGKRKRTRLAQALVIGAALFALAPIARALDTQDIVVEWT
                     EEGKKIAQERVANWKTKDEMVLVPAGEFLMGSDKKTDRLAYRSEIPQRSVYLDAFMIG
                     KYEVTALEYLKFVLATDRLPQLDWRYDGGNFQDTMAHHPIMHVSWYDADAYCKWAGKR
                     LPTEAEWEKAARGVDGRLFPWGSEYAGPTRANFGRTGLSGPVRDRPERLLLYPPIISV
                     DKYENALSPYGLYQTIGNVAEWVSDWYDQDYYKTAPARNPKGPETGTQKAFRGGGWMD
                     STTTMRAAMRNGTDPKTKINWMGFRCAQDAKEDTGTKVSMTKE"
     gene            complement(23853..24785)
                     /locus_tag="PLS35_04920"
     CDS             complement(23853..24785)
                     /locus_tag="PLS35_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNI18029.1"
                     /translation="MGIWRGGCLRALLGCVAIGLLPGLVTPSHADHELPKPPPLWSPL
                     DNVERLALIDVPNGMAEVPAGPFLMGSDPKFDRAAGPQELPQHQVYVDAFSIDRYEVS
                     NVNYLRYVLATGAAWPHYWREQPFPEKMAKHPVIGVSWREADAYCRWRGARLPTEAEW
                     EKAARGEDGRMFPWGNEPAGWIKSNIAHPGSKRGAKYPPLANVDRYDNGVSPYGVHQL
                     AGNVSEWVSDWFDPEYYRRQENRNPQGPASGQDKVFRGGSWNEDPEVARSAGRNSGGL
                     DHWSYLTGFRCATSGESGRQLSSIDPAMGRNTDR"
     gene            complement(24823..25773)
                     /locus_tag="PLS35_04925"
     CDS             complement(24823..25773)
                     /locus_tag="PLS35_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNI18030.1"
                     /translation="MTHRISGWKVGLAAMALGGLLASHPASAYEEITVSDGGTLSGVV
                     KLEGTVPKPKGYNLTTLPDPYYCGRISDGEGWRILQPFNVGPKGEFREVVVYLEGIEK
                     GKSFAEKEVPQIEAKDCLFLPFTTVVRDDQSVTVVNMDPVMHDIQAYETSNLGARVLF
                     NVPLPMNPQHPRNFKDRTEAGMYHKHMAGPPMKELVKLSKGRRIFVMQCGFHAYMESW
                     GVAIANPYFAKTDEEGRFTMTDVPPGTYKLVVWHPYIRTATEKIVTIGPKGTVETTIG
                     VPAPTGRLYANEVLDHAYTRYNVTEEAKKEIDPLLQKQEH"
     gene            complement(25974..>27688)
                     /locus_tag="PLS35_04930"
     CDS             complement(25974..>27688)
                     /locus_tag="PLS35_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18031.1"
                     /translation="FQMPDKILNAPNKYINGGTHTTGSGFNFRAAPFAQRLSNNPDTS
                     KLFSSAIHGDPGTPLLRAYTGDTMVFRLLHQLMNESHVWTIAGHTFLTERYAPDANRK
                     NSIHVGIAERYDLVTKAGGFQGMPGDYIHFNGRTSHFAEGGWGIIRVLDKETADLKPL
                     PRGTNPLGIPATPNSVCPSDAPVKSFNVVALDRPMKLNPKAPDAIEVDFERKIEMTMP
                     EGKIFALEEEAATVAGNVMPNPLTLRANLGDCIKVNLKNKMKASRASFFAPGLAFDPK
                     DSQGLNVGNNAGDQTVAPGESRTYTYYAHPANKETTSLVWDGGNIVVNPRNGLYGAIV
                     IGPRGSQYRDPVTGADVSQKNTWRADVIVDTTLSENAGKRNYRDVALFFQDEDNIIGT
                     AFMPYVQNVAGLTSVNYRAEPYKFREEQGCSLGKIFQPCAVDKPEDPVTPLIEAHAGD
                     ALRIHVIGANSEQNGMFSVEGHEWPIEPYMAGADMISVVEYAGSETLDIFIRGGAGGP
                     YRQVGDFVWSNARLPYTQSGQWGYLRVLPTGDSRIQPLSASGAGARQAEVLPEPQAIP
                     TAMK"
BASE COUNT         5940 a         8774 c         7508 g         5366 t
ORIGIN      
        1 atcagccgta gttcatcacg caccagccgc ggcatcagaa aatgccggcg cacatgttca
       61 cgtcccgccc gcccgaactc cgcacgcgca ccaggatgat gcaggagatg gcgcacccgc
      121 tcggcgcacg cctcgacact gtcgacaagg ttccgttgaa aaggctcagg gaattgcatg
      181 gggatcccgc cggtatttcc ggcgacgacc ggcttttctt tccacagcgc ttcggacacc
      241 accagtccga acccttcccg caacgatttc tgaatcacga catcgcagcc atgttggaac
      301 gcattgactt ccatgcttcc cacaccggcc agattcgtaa agacaaagag gtcggggtcg
      361 ttcgccgcct cttcctccac gcgatccaga atttcccagc cttcgggatc gtcgcccgcc
      421 atcgcgccga tcagcactaa ctgcacctcc gggatctctt ctttgaccag gcgatatgcg
      481 cgtacgactc cgagcggatc cttccaggga tcgaaccgtg acacctgcag cagcaacggc
      541 ctggcgggat ccacgccgga gtcggcgatg acgcggcggc agagatcaat cggcaactcc
      601 atgttcttcg tggccagcgg atcgattgcc ggggcgatga acgccactcg tctcgccgcc
      661 aactgcggca ggacaaattg gctcatggtg aacactaccg cttcatactc ttccaacaag
      721 gggcgcagaa accggctgac ctccttgtcc ggcgccgaac tgtcgatgtg gcagcgccag
      781 atccatttgg catcccgcga tcccgcaaag tgacgtatgg ccgcaggctg gggatcatgc
      841 acgatgtaca gatcgtagtc attgcccagc gcctgcgcac tgcgttcatt gaccttcaga
      901 taggcgtcct gctcggtggt tcccagcccg taatgcccgc cctggagggc gttatgaaac
      961 gccttggtga tggcaaaaaa ctccggctca ccatgaatca aacgccagtc agcctgtacg
     1021 ccaagcgcct gcagcatggg gatataccgc gccaacagtt ccgccacgcc tccgcccgcc
     1081 gccgtcgagt tcacgtggca aatcttgatg ccctgcaaac ttttggctaa cgcggtcaat
     1141 tcttcatact cgtcatcgct catgaggctg cggtagccgc cgagatttgc ttcgatcgct
     1201 ccgcaccggc ggccaatgcc cactttcggc aattgcgtct ttatgtccat gctgcacacg
     1261 tctccttcat ggtcgatcct cacccaaacg tcagccgtac ccattcacca acataatgtg
     1321 tcatggccac gacacagagt ccgatgatca catgctcgcc gatgactttc cagggcggca
     1381 ccctttgcgc ccgcgccagg gcataactga gcaatgccaa gagggtcagc ccccacagca
     1441 ggctggccac aatggccagg tccagaggca tgagcaacac aggcaccgca aaggtggtgg
     1501 caatgacgaa cttcgccaca aacgtcgccg ccgtggacgc ccagatgtcg tgcgtattcc
     1561 cgtggttctt ggattcctcg gccacatgga tgcccaaggc atcggacatg gcatcggcca
     1621 gcgcaatggt gagaatcccc cccaacactg caggcatgga atgggtcccg gcgtgaaggc
     1681 cgaccatcaa gccgagcgtg gtaatgaccc cggacgtcag tccgaaactg aatccgacct
     1741 tcagcgcggt gcgcatgcca tagaccatgt catgttcgac tgctcatcct cgctcctcac
     1801 cggtcgttca tgctcagcag gtttcgcgac ctgatcctcg caggcaggac gcgatccatt
     1861 cattcatcgt ggcgagcacg gttgccactg cacgatctgc ggccaagacc ccaccatagg
     1921 catcctcgct cggagccggt tcaagtgctt cgaaattcct ggcacccagg acacgttgtg
     1981 ccttcacatc caccaagtgc gcgcgcacct gcacccggct ccggctgggc tgctgcagaa
     2041 attcctgctg cacgaccaga ccggacagat ccaactggta atcgcctttg attccggtgg
     2101 gcatcggcac cacggtccgc cagagtccgg tcgtgtccag agatcgaacc aacaacggca
     2161 ataccatgcg tgagggacta tcggcccaca catgattggc gtaatagctc acttccgacg
     2221 gccgccgcag ataggcgatg cgcggctgct cgaatcctgg cgctgcttgc ggtaccgcga
     2281 cgaccaagac gccgtgcatg ccggccggtg ccccggcagg ggccgcttgc cgatccgtct
     2341 cctcacgcat caacacaaac gtccggacag ccgagtcctc cgaccttggc agtacacagc
     2401 cgctggcggt gtataccgcc agcaccaatc ccacccatcc cgttacgcga ctacggagca
     2461 cgtctcatcc tctattcacc agggcccttc ggctgaccct gtcggccaag caccaaaaca
     2521 ttcggctgac gttcaacttg ttgcgccact cgagtcagcg tcgccgtgag ttgtcgcaat
     2581 tcacttacca ggagcccggc atctgccaac gtctgtcgtg aaaattgttc aatgcccggc
     2641 ttgctggtac cgaccatatc cccgacttca cggctcgtcc gtgtcacctc tgccgtcatg
     2701 tgctgcagcg ccgctgcgct tttacccatc cgatccaaga gcgccggcaa ttgtacgccc
     2761 aggtgctccg tgaggcgcgc ggtccgctcg gccgcctctg ccgcatgctg cgcgccgcgg
     2821 tcgacctgac tgctcctggc cgccagcacc ttcgtgacct ctcccaggtc tttcaggatc
     2881 tgccggaact gcgcacgatt ctgttcgtcc aagaacagag acgcattctg cgcgagcccg
     2941 ttcacattgg ctactagatt cgacaggccc tggtcggaca agagtttggc caaggttcca
     3001 tccaggcggc cgtacaaaga gggaacgctc tggataaccg gatacacctg ccccgtcaca
     3061 ggcgtcaacg gcggcgcgtc tttgcttcct cccgtcaggt tcaatgtcac cagtcccgtg
     3121 agtccttgtg tgaccaacac cgcctgcgta tccgttttga ccggtgtccc tccgacgatg
     3181 tccagcgtca cgcggacctc ctcggaattg tccgggttga gaatgacctc cttcactcgc
     3241 ccgacatcca caccgcggta tttgacggtc gaatcgacgc tcaatccggc gacagattcc
     3301 ctggtgtaga cataatagcg atcgtagatg ccgcggtaat cggttttgcc cagccagagc
     3361 accgcaccga gcactgtggc gcccagcagg aggacgaatg cgccgaccgc gacgtagttg
     3421 accttcggtt ccattcgtca cctctcaatc gcgatgcgac tctcccccgc tgtgattcag
     3481 ctgtgcggcg cgcccgcgcg gcccatgaaa ataattccgg acggctggat cctccgacgc
     3541 cgccagctgt tccatggtcc ccatgcccag gacatgccct tcccccagca ccaccacgcg
     3601 atccgcgatg cgccacaacg aatccaggtc atgggtcacc atcacgacgg tgagccccaa
     3661 caatcgcttc agatcaagca ccagatcgtc aaagccggcg gcgatcatcg gatccaagcc
     3721 agcggtgggt tcgtcgagga acaagagttc cgggtccatc acaatggccc gagccaatgc
     3781 ggcgcgccgc ctcatgcccc cgctgagttc gcggggaaat ttgacgccgc tgtcaggcgg
     3841 caaaccgacc agggcgattt ttaatgccac gatatcccgg atgagccggg cactgagcga
     3901 cgtatgctct cgtaagggca ccgccacgtt ttcggccaag gtcatggaac tgaacaatgc
     3961 accgtgttgg aacatcacgc cgaagcggcg atacaaggaa ccgccgtcgg tctcttggag
     4021 gtcccggctg tcagctccca gcaggctgat acgtccggcc gtcggagtca gcagcccgac
     4081 gatttcccgc aagagcgtgg atttcccgca accattgccg ccggcgatgg cgaatacctc
     4141 gccttttctc acgatgaggc tcacatcctg atgcacaatc gccggcccga atcgcgtcga
     4201 gacatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat gaggctcaca
     4321 tcctgatgca caatcgccgg cccgaatcgc gtcgagacat gccgcacatc gataatcggc
     4381 gtctcagtgt gcgcagcgag agacataaga ccctgtcatt tccccaagat taggtcgggc
     4441 cggagaacga atttcaaaca tcttagtagc ccggattgct catgaatgcc gtcgcgaggc
     4501 atccgagacc gaagtccgcc agggcgtctc gcaagcgagg ccgacgcagg cgtatccgcg
     4561 atacgttgag gagggcgaac gagaaacgcc ctgccggacg aaggtatcgg atgccgcagc
     4621 aggtattcat gagcggtccg ggctacaggt tccaccaatt gagaatgatg ctgcacaggg
     4681 catcaaaaat gatgaccaga aagatgcttt gcaccacgct catcgtggta tgtcgtccca
     4741 catcgtccac accgcctcga atctggaatc cctgataaca tccgaccagc gcaatgatga
     4801 cggcaaaaaa cggcgcctta ccgacgccaa tgaggaaatg ccgcaaggcc accgcttcct
     4861 cgaatctcgc cagaaaagcc gtaaagctga cgttgagctg attcgcggcg atcaacatcc
     4921 cgccgaacac acccagcaca tcggcataga cggtcaacaa cggcagggcc acgatcagcg
     4981 ccagcatccg gggcaacacc agcaactcgg tgggcgacaa tccgaatgtg cgcaacgcgt
     5041 ccagttcttc cgtcactttc atggtgccga tgtgtgccgc gtacgccgat cccgaccgtc
     5101 cggcgatcaa aatggcggcg atcagcgggg cgatttcacg cagcagtgag atccccacga
     5161 ggtccacgat aaaaatgttg gtgccgaact ttcgcagttg ttcggcgccc tgatacgcga
     5221 tcacgacgcc gatgagaaat gtcagcaacc cggtgatcgg gagcgcattc actccatcga
     5281 gccggaggct gttcaaggcg ctgcgccagc gcaggtgagt gggcgacgag ccgacccgca
     5341 gcagcgccat cgcactctcg ccgataaacc ccaagccgcg caggaaccgg tcgaggagat
     5401 ttctaataac ggattggaga cgcgccgtgc ccttcggtcg cgccgccaca tgcgccatca
     5461 tggcgtccca atgaaccgac accaggttca tgagctgcgc atgttcagag cgcagtcctt
     5521 cgaccgtgac gtcacagccg cgccgctgag cgtccctgac tgcccgggac aggaccactg
     5581 cgccgcccgt atcgagagcg tgtatcccgc ttccctcaaa ttgcaacgaa ccatcagccg
     5641 gccaatccat caccctgagg gcccgttcgg ccggtccgac atcggacatc gtccaggagc
     5701 caagacaacg cagtgttcga ccgtcaagga aggtgacggc agccgattgc ccccgcggcg
     5761 ccattgtgta agggcgattc atagtctgcg tcaggcggtg tcttgcacca ggtcctgttc
     5821 ctgtctcact gcgagacgtt gctttacggc gacgagcaac ccgccgaatc cagccatcac
     5881 ggtgaacaaa gtcaccagac ctccaatgac cggaacgagt gtcaggagtg aatagacgag
     5941 cagcccgacg acaaagggcc gggcaagtga ggaggaatcg tcctggcggc gaacaaacaa
     6001 ttgccctgcg taggtgatgg caaaaattct tgccagatac gccgccacgc cgtacaacgc
     6061 gagaagcatc acccctgccg gcaacaccac cagcgtcacc acgaaactga tcgcaaccag
     6121 cggcgtgacg attaaggcgg caagccccca tcccacagag ggcgcgggtc gttcgcgtat
     6181 cgccccggtg acctgtcgga ccaattgcgg ccatacacgc agcatgatca gtcccagcag
     6241 caacgtcgat agaaagctgg cgatggccgc gaagactcgc atcccgacca gcccctggcg
     6301 ggcccgctcg gtgctccacc cctccggcaa ggggcgacgt gtgatcggcc catgcaccac
     6361 ggcttcctcg tcgatcgagg gcgctacctc accccaatat cgcagccgtc cgcccactga
     6421 ggccttggat gtcagacgta ccgattcagc cgccacaatg acatcgcgat tgatctcatt
     6481 ggagaaggtc gcatgccatg cgccgacccg tgcatcgcgg ccgatggctc ccgacagctg
     6541 cacggagccg ccgcctgcaa tgacgttctc acgcacagtc gccttctccg tcagatggac
     6601 atcggcaccg gcaagggtgg cgtttctccc aatcgtcccg ctcacggtga cgtgcgcgcc
     6661 agccacgcgg gcatcctggg agatagtgcc ggacaacacg accttggctc ccgccacgat
     6721 cacatcgccg ttcacgacgc catccacaat cacttcaccg ccagccgcgt acagatcccc
     6781 attcacgatg cccgagattt cgacctgcgg accaaacgca aaataatcac cctgcaccac
     6841 ctgacccgct cgcaacaccg cccgctcgcc cagtggtgaa cggccatcgg acttctcggc
     6901 agaaacgggc gccgcgccga aaatcacggc catcgctacg accatgcgag tgagggcgcc
     6961 gatcatcttc atcgaggcaa gacccctgcg ccgtagcgat acaccaattc gccgccatag
     7021 taactggcga caagcaataa caccgaccca cccacaccca ggaacaggac gaggagagaa
     7081 cgttctcgca tccagcccca ataggacaac attttcacac ccagcaggcc ggcaaacagc
     7141 caaaacacga cgaatgcgag ttcctcatgc gtttcgatcg cctgcttggg aatcgtcccg
     7201 ctatgctcca cggcctcttc cgcgagatga ccggtgagca aggctaccaa cgccgcgagg
     7261 accccgagcg ccagcaaaga caaactcgtc gcacgaaatt cttccccacg ccatcgcagg
     7321 gcgaccagat cgaacaacaa gcttgccagc aagagggcga taggaaagtg aacgaccatc
     7381 ggatgaatcg gatgcatgcc cctcctccat tcgtgacaga accgacctgc gcctcccgca
     7441 tcaacttcag gacgagctat gcacacgacc atctcgctcc accgcgatgc ggtaatgggc
     7501 ttgcggtttc acataggcag accgcccccg aagggaagaa gtgcaaggat ggcgccctgc
     7561 tctgagtctg atacgagcaa agccaagcgc ttctcctacg tagcaggact ggacggaatg
     7621 atcgggaaac gacgtcagaa tcggggaggt ctgctacaga gagagtctgc gcctctgtgg
     7681 cagaatgcga cagaggcgca gagcagaacc gacctcagcc tttaggttgg atggacagca
     7741 gatacagatg cttgcggcct ggttgcagga actcctgaag ggtttcgacg accaacccgg
     7801 cctgcttctg gagagtgacc atatgcgatt cctcggtaat gacccggatg ggcccctgtg
     7861 ttcgcacggc gtgatgtacg gcgtcctcat cgggaagatc gcgcacgact gtgtcccgct
     7921 tcaagtaata cacggcatct tcatttttgg gccaaccggg aatgaagagg aacatgggcg
     7981 gtcctgcttc cttcgccacg gatttgatgt actcggtagc tttgcgcggc gaagcggcct
     8041 catcaatcgc aggaaacaca tagttcacca ccaggactac ataggccacg gcaagcacgc
     8101 cgaccatacg caaggcgagc tgaaggcggc tggtgatcgc ggcatacaag agcgccccgc
     8161 ccagggaaac cataatcacg atgaacagcg gagacacgac cggagcggag accagccaac
     8221 ggcggcgcaa cggcgcggcg ccgaagaaca tgcctccgat aaacaccagg gccaggacca
     8281 ccagaaacag tctcaacacc ggcgacatgg ttcgtgagcc atccgacgat tggctggacc
     8341 gatgatagaa atacccgatc atcagtccga tgccgggaac gacggtcgtc agatagggct
     8401 cacgtttgag cgtggccagg ctgaatgccg tgaagaggcc cagcacccac accagcaaga
     8461 ccagttccgt ctgatgcgcg cgtaacggtc gttgctgcca caacaacagg agggcggacg
     8521 gcagcagcgc gcaccagggg aaaaaatccg cccacatcat gcccaggtac caatagagag
     8581 gatgcccgtc tttggacgtc ccggcggcac ccgacaagcc actattccac acggatttga
     8641 tcgcactccc aagaagaaaa tgatgctgat acccgacgct caacagttgc gaataccagg
     8701 ccagcaacgc aatggcccct gccagtccga tccagaagaa tctgtcacgt accatgcggg
     8761 cgtcgcgctg aatcaccgca tacagggcca tgatgagaag aggaagaaaa aatccgtgca
     8821 tctccttgag catggctccc agcgccatac tcaggaaggt caccagatac caccgtgagc
     8881 tccggcccag gatctgaacc tgaatccagg cgaaaagggc gagggtgacc agaaacgtca
     8941 atgtcgaatc gaataatacg cggcggccat accagagaaa gacgtggctg gtggctacaa
     9001 ccagcgcggc ccagaacccg gcccgggacg acatcaactg agtcccgagc acataggtca
     9061 gcaggatcgt gcccaccgca gccaatgaac cgggcaggcg caacgcgatt tcgtgatctc
     9121 cccagatcga ggtcgagagg ttcagcaacc aaaaaaacag cggcggcttg ttgaagtagg
     9181 gaatgccttg ataggtgagg tcgaaaaact cgccccgcct ggccatctcg ccggcgatac
     9241 ccgcatacag cccttcgctg ccttccaata acgagaggtg cagattggca aaataggccc
     9301 acccgctcac aagagccagt accagcatct ggacataccg acgcgtatcc gcctcacacc
     9361 aggagagcag cggcgcttcc tgctcgtgac ctacactgcc cattgacaca tgctcaccac
     9421 ttcgcgacat gacgagagat tccatccatc acagatcttg cggcacaacg gcgtgccgcg
     9481 tggggccaca gtatagtttc cccgctcgcc gattgagaag cttttttcct ggggagcggt
     9541 gtacgtagga cttcaagaag ggtcttgtgg aataggagcg gatcgattgc agggaaggag
     9601 agatattcca ggaacggttc agccaggctg attgcccacg gcatgtcgtg gcgctgccgg
     9661 gttatcagct tgaggcaagg gacaggcaga gacaggtcgg cgctcgccga tccacaggta
     9721 gtacagcacg gggatgacaa tcagggtcaa cactgtcgat gcaccaaccc cgaacagcag
     9781 tgacaccgcc aggccttgaa agatcggatc gagaataatg acgaaggcac cgaccatcag
     9841 cgccgccgcg gtcagcagaa tcggacgggt acgaatcgct cctgcgcgaa tgacggcctc
     9901 caacagcgcg atgccttcgg cttcctgatg ctgaatgaaa tccaccagga gaatcgagtt
     9961 ccgcacgatg atgccggcga gcgcgataaa tccgatcatg gaggtcgccg tgaaatagga
    10021 tccagtgagc cagtggcctg gaagaatgcc gatcaaggtc agcgggatag gcgccatgat
    10081 gaccaccggc gtcaggaacg actgaaactg cgccacgatc agcagataaa tcagcacgag
    10141 cgccgccgcg aaggcgagac ccatatcccg aaacgtttca taggtaatgt gccactcgcc
    10201 gtcccacttc atggcgaacc gctgttccga ccagggctgc acggcatact gttgttccaa
    10261 tcgataccct tccggcagcc tgtactgttc caatttcttg ccgataccca tcacgccata
    10321 gacagggctc tcgcccttgt cggggcctgt gccaccgaca tccgcgataa cgtagacgac
    10381 cggcttctga tttttatggt agatcgcctg ttcacggacg gtctgttcaa cgcggatgag
    10441 ttcggaaagc ggcaccatat gacccgcggt cgttcgcagg gcgatttctc cgagatgttc
    10501 caggccggtt cgctcaatga tcggcagacg aagtttgacc tgcaccggtc gccgctcttt
    10561 cgggatatgc accaggccga cgtcggcacc ttccagtgcg aggcggaggg tccgcacaat
    10621 ttcctctgac ggaatgcccg ccaaggccgc cttggcacgg tcgaccgaaa acacgtactt
    10681 gacctgctcc gcttccaagt agtcgtcgac atcgacgaca ccggccgtgg tttcaaacat
    10741 ccggcgcacg tccctggcga tctcgatctg gcggtcgtat tcagggccat aaatctcagc
    10801 gacgaggacc gattgcaccg gtggaccagg cggcacttcg accactttca cattcgcccc
    10861 atagagccgc ccgatgtcct gcaccggcgg gcgaatccgt tgggcaatgt cgtggctctg
    10921 tgcctgccgt cgatccttcg ccacgagatt gatctgtata tcggcctcgt ggggctgctc
    10981 gcgcagataa tagtgtcgga ccaacccgtt gaagttaaac ggtgaggccg taccgacata
    11041 ggcctggtaa tcgcgcattt cgggaatggt tcgcacatat tgcgtcaggg ccttggtcgc
    11101 ccgggccgtt tcctcgagcg tcgtgccttc cggcatatcg atgaccagct gcaattcgct
    11161 cttgttgtcg aacggcagca ttttgaccac cacgtcgcgc gtccagaaca acaggaccga
    11221 cccggccaac aacagcccaa tgacgcctat caccagataa gccatcaccg gatgcgacaa
    11281 caccggagcc agcaaccggc gatagagtcg ataactgcga ccctcctcaa acgaatcatg
    11341 atcgacgccc gccatgtgta cgcccggtcg ataacacgtc tgacagaacc aggggatgac
    11401 gatgaatgcg accaggagcg acacaaacat cgcaatcgag gcgttcacgg gaatgggacg
    11461 catatacgga cccatcagcc ccgacacgaa ggccatcggc aggagagcgg caataaccgt
    11521 catggtcgct aaaattgtcg gattgccgac ttcgtcgacc gcaaagagcg aggcctcgcg
    11581 atgcgggagg agacggagtg tgagatgccg ataggtattc tccaccacga cgatcgcgtc
    11641 atccaccaga atgccgatgg aaaagatcaa ggcaaacaac gtgacgcggt tgatgctgta
    11701 cccgatcagc atggaaatga acagcgtcag tgccagggtc aagggaatgg caatggagac
    11761 gacgaatgcc ggccggaatc ccagcgtcag cccgagaaag accaccaccg caaccacggc
    11821 gaccaacagg tgccagagca gttcgttggc tttctcatcg gcggtttctc catagtcgcg
    11881 cgtcacggtc acctgcacgt cggctggaat caatcgcccc ttcatttcct cgacttttcg
    11941 aatcactccc tgcgcgacgg tcaccgcatt ggtgccggct tgtttggcaa tcgctaccgt
    12001 cacagctggt gtttcacccg aagaggaaga actgcttcgc gccccttcgc cgaaccatac
    12061 gtactgattg gtttcgctcg gaccatcgac cacctccgcc acctgacgaa gatagaccgg
    12121 ccgttgttca ttgacaccga ccacgacgcc gtcaagatcg tccttcgagc ggatgaaccg
    12181 gcccgtctca accaggaagc tttcgtttcg gctctcaaac cggccggcag gcagcgctaa
    12241 attctccccc tgaatgacgc tggcgactcg caagggtgtc aatccatagg ctttcaaccg
    12301 tgtcggatca atctggacgc gcagctcgcg acctcggcca ccgaccacga aaccactcgc
    12361 cgtgccaggc accttcttcg catcctccag cacctggtcg cccaactgac gcagctggaa
    12421 atccgtatag cgctcgctcg acagcgtcag ggtgacaatg ggcacatcat tgacgtcacg
    12481 cggtttgact tgaaacggcg cggcgccagg cggcaacaga tcctggttgg acatcaattt
    12541 gtcatagagg tccaccaggc tctgctccat cggttcaccg acataaaacc gcacgatgat
    12601 gagcgcgccg ccgggtcggg aaatcgaata gagatattcg accgttttga tttcggagag
    12661 cttgcgttcg atgggtttgg tgacctgctc ttcaacgacc ttcgcggagg ccccggggaa
    12721 tgggagccag acatcggcca tgggcaccac gatctgaggt tcttcttcgc gaggcgtcaa
    12781 cagtaccgcg aacaggccga ggagaaggct ggcgaggatg atcaacgggg tcagtttgct
    12841 gccgacgaag agtgcggcga gacgtccgct ggcgccgagg cgtggggtcg tcattcgtga
    12901 accatcatgg acatccagac aagatccgtt gtgctgtgta ccagaccgcc ctgtcgacag
    12961 cattaccgcg cctggggcgg caaggccacc gcggtcatat cctgcacgag cgcgccgtcg
    13021 cgacctttgc ttccgtcagc gatgacccgc tctccggcat cgacaccgga caaaacttcg
    13081 accccttgtt ccaacgtgcg gccgatcctg acccatcgaa ggcgagccgt ctggtctcct
    13141 ccgacaacga acagactggc cagttcaccc cgttcgatca acgccgattt cggcaggagc
    13201 agcgtcgtac tcatcccctt ctcaagtttg agtcgcccga acatgccgga cttcaggccg
    13261 cctgcctgtg gcaaatcgac tttaatcgtg aaggtgtggg tttgcggatc accggcaggg
    13321 agaatctgag acacggtgcc tttgagcgga ttcgccccca aggcgtcgat cacgacgggc
    13381 agcaacgagc cgactgacac gacccgcaca tcactttcgg ctacggtggc ttccaaccga
    13441 agccgcccgg gatcttccat cttcaggagc ggttgccccg gggaggccaa ttccccgcgc
    13501 tccacctttt tttcggtgat cacaccgtcg aacggcgctt tgacgatggt gtaactcaat
    13561 tgcgccaata ccgccttgcg gccggcttca gccactctga gggcacgcgt cgcattctcc
    13621 aattcctgtt tcgataccgc atctttgcga tacagctctt ccatccggtc ttgatgcgcc
    13681 ttcgcattgt cgagttccgc cgtcactctg gacaactccg cctgcacgtc ccggttgtcg
    13741 agcaccacca gagtctgtcc ctgacgcacg acgctccctt cgcggaccag caggttatcg
    13801 atcattccct ggatccggct ggccaacgtc gcctgataga ccgctgttac ctgccctgtc
    13861 acctcgactg agatcgggac ctggctggct gcgacctcga tcaccggcac ctggatcggc
    13921 tcctgtcccg cagtacccgg ggtcatgttc cccggcttgc cgggttcctg agaaggcccg
    13981 cagccggccg ccaggagcag ggcgagcaca aggcctccaa tgctgaaacg tgtgtcgtta
    14041 ctcgtgaaca ccaagcctcc gcaaaagagc catcatcgga caccagccgg tgaatgacga
    14101 ctggataaga ttcgccgcga caaacgccgc gaaataatta tagtagggat gaaccgttgc
    14161 acccaacacc accgcgatca acacgaacac accagcgatc aaccgaagca tttcgttcaa
    14221 cttcataatc cgcctccttt ctcattccgt atgagtatcc ggacgatgaa aggattcaac
    14281 ggcagttcat tgtgtattca gtcacttagc ctccgagagc ggcaaccatg ccacccggtc
    14341 ggcatcaaaa ccggtattga ccaccgatcg agacaatcga atcctgaaag tctctcagga
    14401 cattggtcga ggtacgtttg ccatactgaa acgacaacat agccgtcgca gccgtcgaga
    14461 tgtggtatcg cagctccgca atcccctggt gcgtggcgtc aaaccgatcg acatggctgt
    14521 cgcccgcaat accgctggtg aatgttttcc ggcgatggat ataggtcaag ccgagatccc
    14581 attccttgtg caaccggaac tccgtaccgg ccgagaccat gtggagatag taggacacgt
    14641 catcggcaaa ttgagtctcc ttgtggccat ccgccagacc tcgttcgtac agatacgaca
    14701 gcgtgagcgt cagccattcc ttcgcgcgat agtccacacg cgagccgacc gtccagaggt
    14761 tcgtatcgcg ttccgcaaac acatcgttgt agcgccggat cccgaagcgg gtgatgaggg
    14821 tcgcggtgac cgtgtcggac aagcggcgtt cgacctcggc gcgccaatga tgcgaactga
    14881 cccgttcttc ctgaaccgag cgtgtgccgg ttcggcgctc gaagttgggg ccaagaaaga
    14941 gattggggat gtatcggtag cgaacgagca ccgacgtcgc ctggtcgagc gaaaagcgat
    15001 cctgaatgcg aaagtcggaa tggttgaaaa tgggattgtt ggtgtaaatc gccccttgcg
    15061 ccttcaccga caattcgttt ttcccctgca tcgtcggcgt ggaatggatg acctccaggg
    15121 cgggctccca gatcacatcc tgaggtttgt cggtcgagac gaccgtgggc agagaaggat
    15181 cttccgtcaa acggagccgg cgcgcggaag aaaattgaaa cgcatcggtt gtatagctgg
    15241 ttttttgctc ggcgatcgcc gaccattcgg cttgcgccgt ccgctccggt gtgaccatgg
    15301 aatatcctgc cacggtccat aacgcgacac ggaatgcgac ggacacaagg acacgctgat
    15361 acggcatcat ccgaatgtag agcccatgta agccttgaac ggaatgcgtt cccccggcac
    15421 ccgcacctca tgcccggaac aacatccacg agcaccttcc tctgagagtc cgacagaatc
    15481 cgcgacctgt caatagagtt cagcctagcc acccggcaag cttcgccagg ttgaccacga
    15541 tcaatatgcc tgcgatggag atggtgatcg aggcggtcac tcgacgcatc accacagggg
    15601 acacccaggt atccgcgcga acacccagta ccatgccggt cacgctgccg agcagcacgt
    15661 agcctaacag gctccactcg acatgaccga actgcatatg tcccaccacg ccgccgatgg
    15721 agatcataaa aatgatcatg agtgacgtgc cgatcgccgc acgggcagga aaacccaaca
    15781 ccagaatcaa ggccgggacg accatgaagc cgccgccgac cccgaacaat ccattcagcg
    15841 tccccaccat caacccgatt ccccccacct tcatccagca ggtgcgcggg aacagcgtgg
    15901 cacaactttc atccgctcca tgctcagccg ccagttcccg ctggcgcgcc atgacggttc
    15961 tgaccagcag caacagcagg ccgaacagca ccagcaatat ttcctgtcga atcatgtgat
    16021 ggacgaacgc gccgccccac gaaccgatca tgcccgtcca actgaatgcc gcggcggctt
    16081 tcggtttgac catcccttgc cggccatatt cccacgcgcc taccaaggaa gacgccccga
    16141 caatcatcaa cgacatggcc gccgcctgct gcagggacat gccggcgata tacaccatca
    16201 gcggcacccc gagaatcgcg ccgccggttc cggtcgcacc caattgcagg ccgataggca
    16261 ccccggacac caccgcgcgc aacgcctgct cgatcatacg actctaccgg tttcccttca
    16321 tgccggctct tccagcttcg caatacccat cgctttaagg atgacctttt cgtaataggg
    16381 ttcgctcacg ccttttttca ttttgcgcaa aaagtatttt tccagggcga tcttcgccaa
    16441 atgaacccac ttgcccttct tcgcccacgt cacattgcgc ggcgccattt gcggcaaggc
    16501 gacaaacgcc acaccggtgt cgcccatgtc ggccaggcag atggcattcc acgtcgccgt
    16561 ttcacgtttc gtgtcgttgt cgatatcggc cttgatgtta tgcacggccg ccgacaccat
    16621 cgactcgatc atgtacccgg tcttgggcgc gccggtcgga ataggcgtct gttccaccgg
    16681 gggaatggcc acacagaccc cgacggaata gatattttta tacttgggat tcgcctgata
    16741 ggcatcgata ttcacaaacc ctttcggatt gcacagcccc ggcgttcccg cgaccgcgtc
    16801 gacgccggca aagggtggaa tgatcatcga gtagcggaac ggaatctcct gcccgccctc
    16861 cagcaccatc ttccccggct gcacctcttt gatcgacgta ttacacatcg gcttcatcgc
    16921 gtgctcggca aattcatctt ccatcaaccg gcgggacgcg ccgacccccg cgaggcccat
    16981 gtggccgata tagggctccg gcgtcacgaa atagatcgga actttcttcc ggagtttctt
    17041 cttgcgaagg tcggcatcga gaataaaggc catttcatag gcggggccga aacaggatgc
    17101 cccttgcgcc gctccaacaa cgataggacc tgggtctttg agaaatgcct gatacgtgcc
    17161 ccaggcctgt tcggcatggt ccacgttgca gaccgactgc gtatagccgc ttggccccag
    17221 cccgggcacc gcgccgaaat tgagcttcgg cccggtcgcg atgatgaggt aatcgtacgg
    17281 gacttcgcct ttcgacgtga tgacccggtt ttgctcgggc tcgatccggt ccgctgtcgc
    17341 gtggatatac tccacccctt ttttctccaa caccggaccc aaggcgaaac taatgtcctt
    17401 ccgggtacgc cacccgaccg cgacccaagg atttgatggc acaaagtgaa aggattccac
    17461 attggaaatc accgtcacct tgtgtttctt cgccaacaat tctcgtgctt cgtaggccgc
    17521 tggtaacccc ccgatggatg ccccgatgat gaccactctc gccatgatgc acctccgtaa
    17581 aagtggaccg ccgctgagac ttgcgcttgg gctttagacg ccagagccgc catgcgtacg
    17641 gccttcacca atgccctgac tttgcacagg ttatgcctgc gatcaatgat tgagaggcac
    17701 gaagcacaaa atggattcat ccaccggctg atagttgtga ctggactgga gcgattcatc
    17761 ttcaagcaca ggtctgctac tcactgttgt gcgacgacac acttttgaca catcgccgac
    17821 ccgaccaagg gatttgtcgg gttgacatca gggggcaggg gtgggtagtg ctataggatg
    17881 atcttgtacg agacccatct gacgtggctt cgtcgaatcc tcgccggcaa aggcctgatc
    17941 attgccctga ttgtgggatt tcttgcagtc gagtccatcg tggccgcctg tttcctcagc
    18001 ctgatgcact tcaaaacctc aaacaattgg gcaacccgaa atgaacaggt cttaattgaa
    18061 ctcgaacggg tacggagtat tgtgtcgggg gcggaaaccc atcagcgcgg ttacctgatt
    18121 accgggtcgg ataactatct ggcaccgtat cgagaagcgc tcgacatgct ccaggaacag
    18181 atccgacgga tcggcagcct gacgagggac aataacttgc agcaggatcg ggtggcctat
    18241 cttgccacac aagtcgacca gcggtcggac gaaatggaac aggccattgc cctccggagg
    18301 actaagggac taccgggagc caaatccatc gtgactcaga atcaactgaa tcggacgatg
    18361 gacacgatcc atgacatcac ggagcagatc cgtgacgagg aggcacgggt gttagcgcgc
    18421 aatcgcgcag actccgaagc ctgggcactc acgaccggtt cactggcgct cgtctttttc
    18481 ctgctgaacg cggtcgtatt cgcgttatgc ggggtggtga tgaaattggc cctctcgagc
    18541 cacgcgcaaa ccgaacgtct ggttgacgca ctccggccct ccacaactcc cgcgacacga
    18601 taagactttc cccgaagcgt gcccgtacga gacgccgtct cgaccatcga tcgtacgttg
    18661 cctcgatcat tcttcaatga acgaggctag gtccgggcca acaccacagc cgccaatagt
    18721 accagcaacg ccaccagcag cgacacgcgc ggtaccagca cggagtaccg cactaacaac
    18781 cgatcctttg cactcctcgc tgcctctgcg gttccgagaa tgcgccgcac ctgtggcccc
    18841 acgaccagat cgtgcaacag tgtgagcgtg aacaagaagg cgacgagtcc gatcttcgtc
    18901 ataaaaatag acggccattg cgacggatca ctcaaaggaa tggctcgccc tgcagctagc
    18961 accgacccgg tggcgaccaa aattcccatg gcgccccaca ccagcgctct gaatcgatag
    19021 gcagccgttc ggaagagtgc ggcgtggcgc gcaaaacccc cgtcccgttt gagcacgggg
    19081 accaacacga cggagaggaa caccatcccg ccaacccaca cgacagccgc taagaggtga
    19141 atccacacca atgcaaacac agggcctcct ttttccgcta tgcctcaacg gcaccatgac
    19201 agagggatga acggggacgc aaggcgggcc acactaccgg tcggcaggac gagcgagatt
    19261 cgggcaccaa tcaatttctc ccaaaacgac caagacctgc gtttcggggt gcacaatgga
    19321 ctgtgcaaga ggagtgtctt tcaagctgca gagcgggcac aaaaactggc acggcgccga
    19381 gcagaacgcc agcccgcagc ccaacccgac tcgaacactc gcggctttgt tcctgcagta
    19441 gctcacccga tcggaacatg acagactctg cctgtaacag tcgagccgat tccacaaggc
    19501 gacgaggctg gacatccgtt gccccccaac tgccgcccac gctccgggaa tacggacgga
    19561 ttcaccgatc gcctgcaacg ccagatcgat gtgttcggga agaccggtga aggtcaccac
    19621 atgatggagc ccggaaccgg tttcttcgat gcgatgcgaa aaggcctgct gtggagaatg
    19681 ttcgccaatc ggaaacggca aggaagaatc gtcgggaaga tacagcgtga tatccaccaa
    19741 cgcgcggcct ccggagatct cgattcggaa tgatgccaca ccggcctgct ttcggcaacg
    19801 gccggtgtgg caccgtcatt ctcattggct tacgactgat gttccgatcc tacactaaga
    19861 cgcctcacct ctcgcgtgct tagaacgaaa tcccgacata gggaccaacc tggaagtaat
    19921 cgttgttggt gttggtcaga cgtgccgtca ggtggtaccg ggcatcaacc ccgactttga
    19981 acgccttcca gaccgcatac tcaaaaccgg caccgaactg cacgcccagg tcgaggtact
    20041 gcgtgcgatt cgacggaggg ctgatgacat gaatatccag accgaccgga atgacccatg
    20101 gcctgaacgc gctgccttcc ataaacttga ttttcggagc gacgtcgatc gtcaccatcg
    20161 tgagctgcac tttctgcgga tccgtcgcca gcgtgtcagt taacgttctg gtcgcaatgc
    20221 tcccggcggc attattcacg cgggaggaat taatgctgtt aaattgcagc ccgatttcac
    20281 cgagcaccca cgtcttgtcc attccgcccc agacatcctt cgacatgacc agatccagcc
    20341 ccgcccccgc ataccatccg gcgttgcctc cattggcgcc ggcgatgcct gccgtatccg
    20401 taaaaatctg cccgctgcga tcctggttca aattcataaa tccgcccttg aagaacacca
    20461 tgttgcccgt gccttcttca gccgatgccg gctgcacacc agcaacaccg agcaccgcgg
    20521 cgatcgcaac ccccgcaccc cacattccaa tgttctgcac gactttccgg acccgcttca
    20581 taatcccctc cttgatgtgt caccgacgcg cctgaccggc gcccgggttg gctccccgtc
    20641 tttccccaac cggcccgaga caggagcagc gcaagcatgt gattcaccct tctgaacatc
    20701 gcggcgattc cccgtcttct tcctccgtcg ctcatccaac acctcagtgc ccctcgtgcc
    20761 ggcctcacac cagccagtca cgcgcctcat cggtcccacg cctgttgccg ctgatgcacg
    20821 agttcacact tgcaacatcc gttggctgat tcgacgtctc tggtcggcgg tcaaccgcat
    20881 ctcggccaag gcgaacagca gccgctcttc gcaggcaatc tgctcacgat agcgacgaat
    20941 cagccggtga agctcatcgg tcagcgctcg caaccctccg caggcctttg gctccactcc
    21001 gtcttggccg tcgctccgct ttctcgccaa ctgttgcatc accgctgtcg catcatcttt
    21061 cagggcctgg tgttcttcca cgaagctctg aaactgctct ttttcttccc gattccggcc
    21121 cagagttctc cggagattgt ccaccagcat ttcctcccgc ttgaaatgca catccaccgg
    21181 gccggtaaaa aattcgagca ggtcccgtag cgtgtcacga ttcgtctgct tcaccgctcc
    21241 gctgccggag cagcagggac tcatcgccgt ctcgatcatt gccagtcgct ccagaatcat
    21301 gtgatgctcc cgcttgagca gcgccaaagg atcgacgagg agactcgttc gcctgctacc
    21361 ttgaggcacc atcgttccgg aatcccatgg acggaagctc caatctgcga gccatacgac
    21421 agcgtcatgc cctgcgcact gtaccgaaca ggtaaccagg ccacactggc atcagccggt
    21481 cggaagcatg atttatctca aggttttgga taggccatta gacctatgcc tgcaggaggc
    21541 gatgcgtgag agacgagaag ctgatgagtg caatgagggg agagccgagt tgtgtgtggg
    21601 ctgtcagcac tgcaaacttg aacgtcgcgt cgccccgacg cgcttcacga acaatgggtc
    21661 acgcaacggg aaagggggtg atcagcgctc ggcaattctg gagaggcggt ctggattcag
    21721 caaggtgatg gttcgcccat caatggtgag gactccctcg tcgcgaaaca cgccgagcaa
    21781 ccggatggtg gtttcgaccg taatgccggc catctcagct acttcttcac gcttgagggt
    21841 gagaccgact cgaacttgat cgtctttttt tttgccgaat cggtcgccga gctccaggag
    21901 caagccggct aggcgctcac gggcggtttt gaatgtgaat tgatcgagat gatccatatt
    21961 gaccccgact tcgttactga ggagctggat cagcttgatt gccagttgag gatttctgtg
    22021 gatgacggcg aggtagcgtt ccttatcgat gacgcacact tgagaaggtt ccaatgtttc
    22081 gcacgtcact tcatgaaggg cccgatcgcc gaatacgtgt ttctcgatca gctcccccgg
    22141 gcccaggatg cggacgatct gtctccggcc tcgggtcgag gaacgcgtaa gcttgacctt
    22201 gcccgagcag agcaagtaga ggcccaggca cagatgccct tcgtaaaaca cagtctgatg
    22261 aggagcgtac tcgagcgtgc gtttgatttt ctgaaactct tggagatcgc tccctgaaat
    22321 gtcgcacaga acgaccttct cgcgaagccc gcaggtgtca caatgttcga tgaggcaggg
    22381 cttccgtttc acgatcggct gattcctgtt accacttcca caccctgctc tcgaagatga
    22441 ctcgcaacaa ggtgagcacc gtctttcacc acgaattcag cgtaggaaat aacgcaatct
    22501 gtcatcgacc gtcaagagga gtctgcgcat gactacttat cctggcgagt ggagatcggt
    22561 ctcccgtata gctcgcactg caggtcaata ctcccttgct ggacatgctg ctgagagtgg
    22621 caggcgatat cgacgggctg cgtaattgcc ggtgagcttg ttggtgatct cggggcttag
    22681 aggcggattg attaccctct tcggtaggct ccattttttc atttgcgcgc gatgatgagt
    22741 gcgccgacgc gaccgcatag cgcaaccgga gggaagggag gccggtcgtc tccccatcca
    22801 gaagacgacc ggctcagaga ttgtggcggc aagcactacg ttctcaccgc cctttcccct
    22861 gttactcctt cgtcatcgaa accttggttc ccgtgtcttc cttggcatcc tgagcgcagc
    22921 ggaatcccat ccagttgatc ttagtcttcg gatcggtacc gttccgcatg gccgctcgca
    22981 tggtggtggt gctatccatc cacccgccac cacggaacgc tttctgcgtg ccggtctccg
    23041 ggcctttcgg attccgagcc ggtgcagtct tgtaataatc ctggtcgtac caatcggaaa
    23101 cccattcggc cacattgccg atggtttgat acagtccgta ggggctcagc gcattctcgt
    23161 acttgtcgac ggaaatgatc gggggataca gcagcaaccg ttcggggcga tcccgcactg
    23221 ggccggacaa tccggttctg ccgaagttgg ccctggtcgg accggcatac tcactgcccc
    23281 atggaaacag tcggccatcc acgccgcgtg ccgctttctc ccattcagct tcggtcggca
    23341 accgcttgcc tgcccatttg caataggcgt ctgcgtcgta ccagctcaca tgcatgatcg
    23401 gatgatgcgc catcgtatcc tggaaattgc ctccgtcgta gcgccagtcc aactgcggca
    23461 aacggtccgt ggcgaggaca aacttgagat actccagtgc ggtgacttcg tacttgccga
    23521 tcatgaatgc atctaagtag accgaccgtt ggggaatctc gctgcggtaa gcgagacgat
    23581 ctgtcttctt atcgctcccc atgaggaact caccggccgg aaccaggacc atttcgtctt
    23641 tggttttcca attcgccacc cgctcctgcg cgatcttttt cccttcttcg gtccactcca
    23701 ccacgatgtc ctgcgtatcc aacgcccgcg caatcggagc cagcgcaaag agcgcggcgc
    23761 caatcacgag ggcctgcgcc aatcgtgttc gtttcctttt ccctttctgc agtcgtttca
    23821 tctatggcct cctcattgtc ctgccgtcac aattaccgat ccgtattgcg tcccatagcc
    23881 ggatcgatag acgaaagctg acgaccactc tcgcccgatg ttgcacaacg aaaccctgtc
    23941 agatagctcc agtggtccag ccctccggaa ttgcgtccgg cggaacgggc cacctcggga
    24001 tcctcattcc acgatcctcc gcggaacact ttatcctggc cggatgcggg tccctgggga
    24061 ttgcgatttt cctgtcgccg gtaatattcc ggatcgaacc agtctgaaac ccattcactc
    24121 acattgccgg ctaattgatg cacgccgtag ggactcacac cattgtcgta gcgatcgaca
    24181 ttggccagtg gcggatactt cgccccccgc ttggaccccg ggtgagcgat gttgctcttg
    24241 atccacccgg ccggctcgtt gccccaggga aacatgcggc cgtcttcacc gcgcgcagcc
    24301 ttctcccatt cggcttcagt cgggagccga gccccacgcc accggcaata ggcatcggct
    24361 tcccgccagg agacaccgat caccggatgt ttggccatct tctcggggaa tggctgctcc
    24421 cgccagtagt gcggccaggc cgcaccggtc gccaacacgt accgcagata gttcacgttg
    24481 ctgacttcgt accggtcgat gctgaatgca tccacataga cttggtgctg ggggagttcc
    24541 tgcggtcctg cggcccgatc aaacttcggg tcgcttccca tcagaaacgg gcccgccggc
    24601 acctcagcca ttccgttcgg cacgtcgatc aacgccagcc gctccacatt gtcgagcgga
    24661 gaccagagcg gcggcggttt cggcaattca tggtcagcgt ggctgggcgt caccaagccg
    24721 ggcagcagcc cgatggcaac acaccccagc agcgcccgga gacacccacc tcgccagata
    24781 cccattgcct tgctgatcag acgtgctcct tgcgcgacca acttagtgct cctgcttttg
    24841 gagcaaagga tcgatctctt tcttcgcttc ctcagtcacg ttgtaccggg tataagcgtg
    24901 gtccaagacc tcattggcat acaaccgccc ggtcggcgcg ggaacgccga tcgtggtttc
    24961 aacggttccc ttaggcccaa tcgtcacgat cttttccgtg gcggtcctga tatagggatg
    25021 ccacacaacc aacttatacg tgccgggtgg cacgtcagtc atggtaaacc gaccttcttc
    25081 gtcagtcttg gcaaaatacg ggttggcaat cgccacgccc cagctttcca tgtacgcgtg
    25141 gaagccgcac tgcatcacaa agatccgtcg gcctttgctg agcttgacca gctctttcat
    25201 cggcggaccg gccatatgtt tgtgatacat gccggcttcg gtgcgatctt tgaaattccg
    25261 tggatgctgc gggttcatgg gcaagggcac gttgaacaag acgcgcgcgc ccaagttcga
    25321 cgtttcatac gcctggatat cgtgcatgac cggatccata ttgacgacgg tcacagactg
    25381 atcgtcacgc acgacagttg taaacggcag gaacaggcaa tccttggctt cgatttgcgg
    25441 cacctctttc tccgcaaacg acttcccctt ttcgatcccc tctagataga cgaccacttc
    25501 gcgaaactct cccttggggc cgacattaaa cggctgcagg atgcgccagc cttctccgtc
    25561 ggaaatgcga ccgcaataat agggatcggg caacgtggtg aggttgtacc ctttcggttt
    25621 cggaacggtt ccctcgagct tgacgactcc cgacaacgtc ccgccatccg acacagtgat
    25681 ttcttcatag gctgacgcgg gatggcttgc taagagccct ccgagagcca tcgccgccaa
    25741 gccgaccttc caaccagaaa tacgatgcgt catgtgttgc tgctcgttcg ttgctgtaat
    25801 acccaatgtc acaaccggcc tcctctgtga tgggttgttg tatgctcgac ctctcatcgt
    25861 aatcatacag ggaacccatc actgaattcc ttcttcgata ctcaaaacgc agatggggga
    25921 gctgcgtgat acaggcagct cccccatccg gtgttacgcc atgacgtgcc tgcttacttc
    25981 atggcagtcg gaatcgcctg cggctcgggc agcacttcgg cttgacgtgc acccgcacca
    26041 cttgcgctca gaggctgaat ccgcgaatcg ccggtcggca acacgcgcag atagccccac
    26101 tgtccggact gcgtgtaggg cagccgtgca ttggaccaca cgaaatctcc cacctgacga
    26161 tacggtccgc ccgcaccgcc acggatgaag atatcgaggg tctcagaccc ggcatactcc
    26221 accacgctga tcatgtcggc acccgccatg tacggctcga tcggccactc gtggccttcg
    26281 acgctgaaca tgccgttctg ctcgctgttc gctccgatca catggatgcg gagcgcgtca
    26341 cccgcgtgcg cctcgatcaa aggcgtgacg ggatcttcgg gcttatcgac cgcgcaaggc
    26401 tggaagattt tgcccaagga gcacccttgt tcttcgcgga acttgtacgg ttcagcgcgg
    26461 tagttcaccg acgtcaaacc ggccacgttc tgcacatagg gcatgaaggc ggttccgatg
    26521 atgttgtcct catcttggaa gaagagggcc acgtcacggt agttccgctt cccggcattt
    26581 tcagacaagg tggtgtcgac gatgacgtcg gcccgccatg tgttcttctg tgacacatcg
    26641 gcaccggtga ctggatcacg atactgcgat cccctcggac cgatgacgat cgcgccgtac
    26701 aatccgttgc gcggattgac gacgatgttt ccaccgtccc agaccaacga cgtcgtctcc
    26761 ttgtttgcag gatgcgcgta gtaggtatag gtgcggctct cgcccggagc cacggtctgg
    26821 tcgccggcat tgttgccgac gttcaagccc tggctgtctt tcggatcgaa ggccagaccc
    26881 ggcgcaaaga acgacgcccg gctcgccttc atcttgttct tcagattcac cttgatgcaa
    26941 tcgcccagat tcgcgcgcag cgtgagcgga ttcggcatca cgttgcccgc cacggtcgcg
    27001 gcttcttcct ccagcgcgaa gatcttgcct tccggcatgg tcatttcaat cttccgctcg
    27061 aagtccacct cgatcgcatc cggggccttc ggattgagct tcatcggacg atccaacgcc
    27121 acgacgttga agctcttcac cggggcatcc gatggacaca cggaattggg agtcgctggg
    27181 atcccgagag gattggtccc tctgggcaac ggcttcaagt ccgcagtctc cttatcgagc
    27241 acgcggatga tgccccatcc accttcggcg aagtgtgacg ttctgccgtt gaagtggatg
    27301 tagtcacccg gcataccctg gaatccaccc gccttggtca cgaggtcata ccgttcggcg
    27361 atcccgacgt gaatcgagtt cttccgattg gcatccgggg cataccgctc cgtgaggaag
    27421 gtatgtccgg cgatcgtcca gacgtgggat tcgttcatca gctgatgcaa caggcggaac
    27481 accatggtgt cgcccgtgta cgcccgcagg agcggcgtgc ccggatcacc gtggatcgcg
    27541 ctgctgaaca gcttcgaggt gtccggattg ttggacaggc gctgtgcaaa cggagccgcg
    27601 cggaagttga aaccgctacc ggtcgtatgc gtaccgccgt tgatgtactt gttcggcgca
    27661 ttcaggatct tgtccggcat ctggaagg
//
LOCUS       DAOQEW010000027        27471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_397_length_27471_cov_8.641523, whole genome shotgun sequence.
ACCESSION   DAOQEW010000027 DAOQEW010000000
VERSION     DAOQEW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27471
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_27471_cov_8.641523"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLS35_04935"
     CDS             <1..349
                     /locus_tag="PLS35_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNI18032.1"
                     /translation="RIGGIAIFLGTARDRSKGKDVDGITFEHYEGMAQKKLREIRERA
                     LKDFGIIELSILHRYGEIGIGDNIVLIIAGAEHRAEAFQACKWAIDELKQITPIWKLE
                     HTPEGEVWVEEHP"
     gene            397..1458
                     /gene="moaA"
                     /locus_tag="PLS35_04940"
     CDS             397..1458
                     /gene="moaA"
                     /locus_tag="PLS35_04940"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNI18033.1"
                     /translation="MDHTPDTPVPTIYDRLGRPLRSLRLSVTDRCNLRCKYCMPEDDY
                     AWLPRDTILTFEEMAELTAVFTELGVDKVRLTGGEPLLRRDLPRFVRQLSENRRITEI
                     ALTSNGVLMADQAADLSFAGLNRVTISLDTLRADRFRTLTKRDLHHQVFDGIKAVVQA
                     GFPSLKFDTVVIKGYNDDELIDLIEYGKTVGGEVRFIEYMDVGGATDWSMKQVLSKAE
                     MLELVGRHYGGVKPVVENSAAPAERFVLPDGTIFGIIPSTTTPFCRACDRSRLTADGM
                     WYLCLYAKDGLDLRAPLRQGRTRDELKALIIAAWEGRADRGAEERKALEVLGLREQRL
                     IEINRLREDPHLEMHARGG"
     gene            1519..2226
                     /locus_tag="PLS35_04945"
     CDS             1519..2226
                     /locus_tag="PLS35_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18034.1"
                     /translation="MFDPHTLTILSLGFVLGLRHALDADHLAALSTVLAERPTVQAST
                     AIGFFWGLGHTLMLLCVGTLLLALNLTIPESMANLFEFAVGGMLVALGLSLARRVYRE
                     QWHVHTHEHGGRPHVHLHSHHLQADHTHGHWYQGSLRPLAIGMAHGLAGSAALMLVVL
                     STVTGMAQGIGYIVVFGVGSILGMVGVGAVLSLPVIYSVTVGPRAYWMVQGLTCLASI
                     GLGVVMMVRIGLAGGWS"
     gene            2426..3214
                     /locus_tag="PLS35_04950"
     CDS             2426..3214
                     /locus_tag="PLS35_04950"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNI18035.1"
                     /translation="MNVRVLGCHGSGQLVPGENGPVQCGTCGFLINEELLVDAGTVGS
                     RLYLEEQRRIRLVLLTHLHFDHIRELPTLADNLVGEIEEPVVIAAIPEVLDGLRRHIF
                     NGTVYPDFFRLPDPVKPVFVSRELLPGQETVLCGLGVTPIPVNHVVPTVGFLIREQRR
                     TVLFSGDTYQTDALWQAAGDVDALQAAFIECSFSDEMAELARVAKHLTPALLAEEFAK
                     LAKPEVPVYAYHLKPRFRAQIRQELGGLGIRHLTALEEGQTLVL"
     gene            3225..4061
                     /gene="accD"
                     /locus_tag="PLS35_04955"
     CDS             3225..4061
                     /gene="accD"
                     /locus_tag="PLS35_04955"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNI18036.1"
                     /translation="MAWFKKGKTEEAEPPKRSKTAEGMWLKCNHCREIVYRKEVDRNN
                     KVCPKCDYHFPISVIERINLLVDLGTFKEWDPELEPQDPLQFQDTRSYKDRIKAQQEK
                     TGRKDAMAIGQGVINGRKVALCVFDFGFMGGSMGSVVGEKICRAVDRALEARMPLILV
                     TASGGARMQEGILSLMQMAKTSAAVAKLGEAKLPFISILADPTFGGVTASIAMLGDVI
                     IAEPKALIGFAGPRVIEQTIKQQLPDQFQRAEFLLDHGMIDMIVERKQLKEAVSTLVG
                     HF"
     gene            4142..5524
                     /locus_tag="PLS35_04960"
     CDS             4142..5524
                     /locus_tag="PLS35_04960"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNI18037.1"
                     /translation="MFATAFPDDHFRILVSFDVNMQCESPVAPMTYPATVQFLYGLQL
                     HGIKLGLETIRTLLARVGDPHRQYPVLHIGGTNGKGSTAAMTASILQVSGHRVGLYTS
                     PHLIDFRERIRVNGEMIPESRVIHGVELLRAASAPDLAPTFFEFTTALAFHHFAECQV
                     DVVVLEVGLGGRFDATNVVEPLAAAITTIGLDHEAYLGSTIEAIAMEKAGIIKPGVPV
                     VLGRIGEPGSRVIEERASTVGAPVLRLDRDFRCEGSSPADCRYTGFGLRLEHLSCPLQ
                     GRFQIDNVACSLALIELAHERGVTVTETSVRAGLQQVAWEGRLEIVGQEPTVMVDGAH
                     NPAAAAVLAEYLSEWKRVSPAARLCLIVGMMRDKHPREFLAPLLPLIDTLILTQADLP
                     RAATGSELRLLLEHDAPFAQVATTPSDALAMAKHSAAASDVVCVTGSLMLVGEIKALL
                     RGDSLSPVRG"
     gene            5526..8012
                     /gene="lptD"
                     /locus_tag="PLS35_04965"
     CDS             5526..8012
                     /gene="lptD"
                     /locus_tag="PLS35_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNI18038.1"
                     /translation="MGRDGYSVSTWQRLISHFPCILSISILFGLFPLFAPSAWGESTV
                     TTVSPTNQPLTVTAERIDHLQEREVYEADGSVVVTQGNLRLTADHVTINALPGTLTAT
                     GRVHFWDASSDLKSERLELNVNTEAGIVANGSMFIRPSNSFFTGRSIRRLSEDHYRIK
                     EGTFTNCDAKEGEVPAWRFTFEDLDVNAGESIGMKRAWLCANDTPIIPVPTLTYPLSN
                     RHSGFLIPTPGYDNRFGLHYQQGYFWAINPSQDLTIAPSYYNNLGYGSDFTYRYYLDR
                     RSSGQWFASFLQQTKLPNVSGVDQVSSDERRLRGLISGQHVQQVTDTLLVRGQASFVS
                     DRQYLQQLSNSGTQRASPSGESTLLATQRLPYGSAYFLGQYLQPLNSGGPDTFQRLPE
                     VGYVLPNTSPFGMPVLLNLDTNFVNFYREQGFAVNRMDFMPGVTTEVMDVGHVIGLTP
                     HVKFKEVYYTRGIQEASPLHRETFWAAMDATSKLSRRYTGGEGGSFLHTIEPSVIYEY
                     VPGSDQSKIAQIDQIDDIPKKNLLTYALRTKFLEQQLNGQSFNWLDLTVAQSYHVGAV
                     QTRAREFTPGVLPFLGSLTQPLQPATVDVQGRKMSDVWMRAVIGNTAPEFFRPPAINE
                     ALGKNVTGGQMLPPTNSYLQVDAFLDPYRGTFSQWNTDLRVQQSNYWYVEVGQRFSHD
                     GNRVRRGDIWNPVSFNEVYAPTKEIQFATAGGGFRTPWGWTIGAKGYYDVKGGRSPEF
                     DVVALYQNPCKCWSLGLYYLQFPDRQSYSFMFSMTGIGWTENFGTIVARTILGPLLVG
                     DRGLPWAAPGGPYGRVQNALPVQSMPMGRY"
     gene            8055..8621
                     /locus_tag="PLS35_04970"
     CDS             8055..8621
                     /locus_tag="PLS35_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18039.1"
                     /translation="MIETKRAAGIRLNRRTVLTTCLLMLAIAVQEIYPSGLSATEHMA
                     HLERAAVFLKAGDYRQALEACQAEVKDRPSARSYLYLTYAYHALDGYLQYLANTDQWV
                     RVEHLYVNLSTGRVEDLLDPPDVLARIAKEVIQQAAQRQADVTAALAGRLDETLVKQL
                     WQQQTAWRQARPDDWWSAAPPQWQWAAP"
     gene            8724..9071
                     /gene="trxA"
                     /locus_tag="PLS35_04975"
     CDS             8724..9071
                     /gene="trxA"
                     /locus_tag="PLS35_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNI18040.1"
                     /translation="MAGDALKATDATWDEDVMKASELVMVDFWAVWCGPCQMVAPIVD
                     ELATEYAGKVKVRKLNTDENPEIAGRYQVMSIPTILFFKNGQAVERLVGARPKRQFKE
                     IIDTLLAQHSGAA"
     gene            9090..10775
                     /gene="recN"
                     /locus_tag="PLS35_04980"
     CDS             9090..10775
                     /gene="recN"
                     /locus_tag="PLS35_04980"
                     /inference="COORDINATES: protein motif:HMM:NF008121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNI18041.1"
                     /translation="MLTELRISNFGVIEQLAVTFGAGFIVFTGETGAGKSLLIDAVTL
                     LVGGRASAEHIRAQAEEAELEAAFHLPPEHPLLALLRNKGFARSGETDILIRRIISRT
                     GRNRTYLNGNLCPVHLLEELGGALVDVHGQHEQQSLLSPSAQMDALDAFGGFQPLRQE
                     YQHAYRDWQDRVGDLETLTAQIAQRREREDLLRFQFQEMTDAGIEAGEDARLEQERPR
                     LMHSQQLGELSDQLHELLYAGDQGVLSLLAGARKLLAKMESLDATTVEWIKTMDEAVV
                     PLKDLADQVRRYRDHVEANPSRLLEIEQRLDRLHRLAKKYGGSLDAMLAWQDSLRAQL
                     AELDHAETHLQERTRAVEQAVLSLRDVADRLSRKRLDAAKKITAQVMKELGGLRMERT
                     RFSVEVSRLSGDAPYGQTGQDTVEFMFSANPGEPLKPLAKVASGGELSRVMLALKTIL
                     AESDQVPVLIFDEVDAGVGGAVAEVMGVRLRSLSRHHQVLCVTHLPQVGSQAHAHYLV
                     EKWVRQNRTMTQVRLLDLDEREEEVARMLAGVTVTKKARAAAAEMIASAKDRQ"
     gene            complement(10783..11925)
                     /locus_tag="PLS35_04985"
     CDS             complement(10783..11925)
                     /locus_tag="PLS35_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18042.1"
                     /translation="MGTQASTEQISIKPSVLVVDDETGPRDALKVILRPFFTIHSADN
                     AKSALRVLKDQHIDLITLDQKLPDRQGIDLLQDIKQDYADIEVIIITGYGSLKSAMEG
                     IRHGAAGYLLKPFNVTELITLINQTLEKKQRLDYLRSFLKTSTALWGTEQEAAEAWKD
                     LQSKYFAIGNSSTDATGTAADVTALIPLLSDLLEAKDRQLLNHCSRVSFYASLLANQL
                     TLPLPDQKALALGAFLHDIGKISLSNYKYSADDDESLKTNTHGKECFETGARMLLPLG
                     FQAEVGQIVAYHQERFDGVGNPHGLEGEGIPLLARIVAIAQAFDNLTVDMPGHQPTSI
                     DEAIRQILLQADTYFDPKLTELFAHVVRECKSSLPALAIAKTSPTN"
     gene            complement(12112..13527)
                     /locus_tag="PLS35_04990"
     CDS             complement(12112..13527)
                     /locus_tag="PLS35_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18043.1"
                     /translation="MKKRVLLVDDEPRVRASLRTVLEPTYEIFEAADAAEGLRSFKHD
                     APDLVLLDVILPGADGLSALQTMRTENRAVPVIMLTGTKAVKTAVDAMKLGAADYLSK
                     PFDVEELQIVIERTLGKQELEQEVRQLRAQVVQRYAFHNLIGKSPAMQEIYAKIEQVA
                     DSRTTVLVTGESGTGKELVAKAIHYNSARRERPFVALNCAALPETLIESELFGHEKGS
                     FTDATARRVGQFELAHTGTLFLDEIGDLSPATQAKLLRVLQEREFTRVGGVQSIKVDV
                     RIVAATNKNLDEMVRKNQFREDLYYRINVIALYLPPLRERGEDIALLAKHFLSKRIEE
                     EKRPSQEFTKDALELISHYPWPGNVREMENIIEQAFIWSKGSTVITPEHLPNILRTDT
                     RSSSLRDDTLAGRLSLEKAVMEFEREIILDALKRTTYVQTHAATMLGISRRMLKYRMD
                     TLGISRPDSGGGNAEASLPQE"
     gene            complement(13529..14683)
                     /locus_tag="PLS35_04995"
     CDS             complement(13529..14683)
                     /locus_tag="PLS35_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18044.1"
                     /translation="MTALSQYTRLPEVALWIRESTGERYRLLASYGHNHPSSLPSTLT
                     GDHPLVAGLSAKPYVCHGSENPSKPLGLAMATARASLCLGLRTKGRLLGICILGPLDT
                     KAQVGEELLAIVDAITHIAGNSLDHYLTQDDLRRSSTLMRRTDRLRSLEIMAGGFAHE
                     IRNPLTSIKTFVQLAPQRQQDPVFIKEFSKLAIEDIHRIERLLHEILDYASYMTPRPT
                     EVDLNDLVASCIGFISSSAAHRGIQVQTHLSPQLPLLLLDRQQIKQVLLNLLLNALDA
                     MPNGNGIIRVHTRLLSEAGRPPWVQLQVEDEGCGIAPDHLEHIFDPFFSTKHSNSASE
                     GAGLGLTTAHQIVREHGGTLIVESRLGAGSTFSVNLPVPDSAPTVSAARE"
     gene            complement(14919..19100)
                     /locus_tag="PLS35_05000"
     CDS             complement(14919..19100)
                     /locus_tag="PLS35_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18045.1"
                     /translation="MSLPEPSLHILLINENADEIKLVTTSLRSFFVGCRIEAGYSSED
                     ALAFTRQGDWQIILIDQDLSPERGLDLLARLRRNAPYAAILLQTNESDSHTAVQALQH
                     GADFLLFKRSPGFLTELLFSVQEALEKRDLQMKLDHTFQRHLRVIETLSDLLYELDAD
                     GRFVYVSPTVTAMLGYTAEELAGRHYSLLLPPQQEPTARFRLNERRAGSRSTRRVELT
                     LYRKALPEAPSVPIIVEVTAKGLFDSTNRYLGTVGLLRDLSQQKAQQNRLAELESRLQ
                     ETDRQLTLSREAARVSRQLQQPLTSLLQDSQRLLTSIQHSRFEQHVETMVAQASQASQ
                     LGHQLAEAIHAPPTELILLDLNEVLQTVAQTTHGETEDSQFLLTTQFAANLPLILGSR
                     ADLEDLTHILLSYACRSTPDNTRPSLTLHTAVLAVETSGSPGQEVASLSRPLRTYASF
                     TIRGSASSLRLPPDSQSGHDRSAVSLFQAHRIVQVHRGAIEVEHTPESGLAITVRIPA
                     SEDFSLTGTPATTISPPPTDIHKPGRDTSQRPERRRSERKPFSLPVQLSIGGTTLRGL
                     LRNMSTEGALLTIRGMESSIHLQPAYVVIKTPVSFLELQGVVHERPSASTELSLPAIK
                     DFVISFALAVEHERNVLRSLLEGLQEGSPQITFEGLILPLPIAQTIQHRNLLPLREMP
                     EDRREAARLSVSCPVYITGSDAASSRPFGKIINLNLNGACIELPTEVVLTEHSRVIHL
                     NPLESITMVPGTSQAPEAEQPLTAQILWNDSAERRLKLRLAATGHHLQQIGIRFDTLS
                     PAQEQRLRSIMEAEFRAEQHLAETPSDAPVLTVSHTVRNREGYTIALRHDFPTGGGSE
                     HDPLVLLCPGYGTTQHAYVALAYMLAGRGFHVVRYDHSRHIGLSDGDPSQTTFTSLED
                     DLDTVLTFVQEHWPERPLTILAPDLPARIALRRQDWHRRIRRLLLLNPTLDLGQCLQT
                     LHHRDLLQEHLNGTCLGLGNLLGIPLDIDHFLADAVAAQYTDVRVLHNELAHCETEVV
                     VLTGGQDGPDCSIPPPPQPLVNDLLSRLGVKGRKVLLPSSTLTSGPQPPSTLRASWQQ
                     VSHLCQAGQALHEPGRVMLPSLLLATSTRARFERDQLRHKHAIGTTSNERLWTAHTEL
                     TRALDELPAHWQSIDQLYQLLQPLDGNVALLDIGCGMHSFARLLLLNLSYRLRAQTWH
                     HNHPLRYVGLDFSSASLHAARASTKEALQHLDELFSGRISAPTPVTQQWILGRSADAL
                     PFADCSFDRVVANFSLSFVRSPVHSLREFFRVLRPGGKLILKAFTPATDLARLYRPAL
                     HEIGIDAFSGAPRLALHRMGQCAVALRTGQLHTFEEESLTTLIARFTPLTPRLVRTLA
                     GQILLAAAEKPDSSS"
     gene            complement(19480..20481)
                     /locus_tag="PLS35_05005"
     CDS             complement(19480..20481)
                     /locus_tag="PLS35_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18046.1"
                     /translation="MNRALLLVVMFLGVTSLAYADGGHDHHAIPTMPSLGETKLTIDE
                     QTVTLTFGPVDLPTGHDGDLASSMPKKVFQLPEDTYMIGYKSEVFTKDGKPLPKNYLH
                     HILMLNNDKPSVSCDGEPLFFAGAGLEMTEARFPDGYGVKLGKGQNLMSVVAFYHKAP
                     PTKDVMARFTMYVAPKGAKVQEMDVYQVGVNIVCYSKFAQRGPDQTDEGIEIKSGVSV
                     LSAPLKFNMDGCVKFAYPHGHDELLMVALENKTSKETLLRTVPEVDADGTFRDFRPHQ
                     VYRDSKGFSVTKQDDYEMVMVHHHLLQNPNIQHGMGNYLLYMTPGACQQSSTTAAAH"
     gene            complement(20557..21462)
                     /locus_tag="PLS35_05010"
     CDS             complement(20557..21462)
                     /locus_tag="PLS35_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNI18047.1"
                     /translation="MESHYFRPLSLRVRTWLLLGPLLGMLYLPTARAGEPHDAETTLR
                     RLVQANAERDLATMSKYMSHDVDAIGYTIDGRKYIGWSDLAADMRSEFDSVVKLEIPI
                     TDLRMWNNKDVAWFAMELDYIRYSDPADLAQRMVLHLRETGVLEQRNGRWILVAWHES
                     TRHHDSGTEQTAANGAALLHRASDAQPAVPNGVDLSGEWEVTEIEDNKKYVATLNAQG
                     TGPYTWQGGQFSTTSFNDRRWQGTWKQTGNDREGAFEVVLSEDGTEAKGIWWYVRVGT
                     RDNIPPRQHGGSYLWKRLTPPPTAP"
     gene            complement(21549..22355)
                     /locus_tag="PLS35_05015"
     CDS             complement(21549..22355)
                     /locus_tag="PLS35_05015"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNI18048.1"
                     /translation="MALAQGKWDWTGYGQTDRGLVRSTNQDAFLVDNRRRLWAVADGM
                     GGHPGGDVASRIVMDTLADFSPSLIRNSDNLDEDHTEQQSFRLTTMAEQAHDAVRAYA
                     DRHPSVYGMGTTLVMAHLMPLPAPRLLVLNVGDSRAYLVRAGRITQLTRDHTLIDDYV
                     REGRLTPEQAARHPDRHVLTRAIGLEPVLQIDLFPVDVFVGDVLLLCSDGLTKMLPDE
                     RIVEIAQSHRDSPSLTTQALIQAAIDTGGIDNITVVACAMTSLIDQGNIH"
     gene            complement(22357..23130)
                     /locus_tag="PLS35_05020"
     CDS             complement(22357..23130)
                     /locus_tag="PLS35_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNI18049.1"
                     /translation="MLTSTFVHLKGIGPSTERRLWESGIADWATFRREAHLTGIAPAR
                     KAAYDAELALAQDHLDLHNPRYFADCLHTRDHWRLFQTFGARALYLDIETTGLSAREG
                     QVTIVGLHRQGRMRTLIHGQDLTLEAIQEELDQADLLITFFGTVFDIPYLQTCFHGLR
                     VAIPHFDLCFAARRVGLQGGLKSIERELDIARESDLLQLDGLEAVRLWHHYRAGHDEA
                     LDQLVRYNAADTQNLEPLAACLYERLVLRYGPQSLGLVG"
     gene            23247..23657
                     /locus_tag="PLS35_05025"
     CDS             23247..23657
                     /locus_tag="PLS35_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HNI18050.1"
                     /translation="MTTNGTMKRIRIKVGGLQLEAELKGTRTAEELYRALPAEGPLNV
                     WGEEFYFKIPGVKDHRETATTQVKIGDVAFWGAGQVLAIFFGRTPMSMGADPVPADRV
                     NVVGRVLGDAATLRQAMNASMIRVERIEPTQPVG"
     gene            23693..24283
                     /locus_tag="PLS35_05030"
                     /pseudo
     CDS             23693..24283
                     /locus_tag="PLS35_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            24318..24758
                     /locus_tag="PLS35_05035"
     CDS             24318..24758
                     /locus_tag="PLS35_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18051.1"
                     /translation="MRVSRWIALVVVVLSCSACAHEKPKPLVPLELAYGAKADANALN
                     NQGRQAFLAGQMAEAKDFFGQVIKIAPDSGQAHYNYGLTLNALGQTDQARQEFIEAAN
                     LAPGDKVIWDSPALRPFGNPEVPKGPAREHPYGTSRPTIGSGPR"
     gene            24793..25260
                     /gene="bcp"
                     /locus_tag="PLS35_05040"
     CDS             24793..25260
                     /gene="bcp"
                     /locus_tag="PLS35_05040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNI18052.1"
                     /translation="MASGVEVGAKAPEFSLPDQDGNLVTLKSLKGKQVVLYFYPKDDT
                     SGCTKEACDFRDSLAAIKKTGAVVIGVSKDGKTSHQKFIAKYGLPFALLSDEDTAVST
                     AYDVYKEKSMYGRKYMGIERSTFVIDATGRIKASFRKVKVPGHVEEVLAALKA"
     gene            25364..25552
                     /locus_tag="PLS35_05045"
     CDS             25364..25552
                     /locus_tag="PLS35_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18053.1"
                     /translation="MPGLGHLYIGEKRGGPLLAAGLLLIIMWINWFSVALVPLFGLVL
                     FCMGDTYLTVSRDRGLFS"
     gene            25592..26458
                     /locus_tag="PLS35_05050"
     CDS             25592..26458
                     /locus_tag="PLS35_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18054.1"
                     /translation="MAEVVGLGLRIFGVLLCAALALATTGEAAGKVSGTLILHDALTL
                     PNHSVRIEAQVAVTAAGPPLSGLMLHLQIDGKDVATAKTNEGGRAAFDYLAKMRGTNV
                     ISVSIDPDAPVAAEKVDATLCVWERRRPIVVVELDALIQQSPAPATPAASPGIGVTDD
                     TVPTPVPEAAEELSRLTQYYYNVVYVSNNEQGPMGNVSSGQVRRWLEAHQFPVGFVAT
                     PTSGGLNATLDALKHDGWTTMKSGIGRTRGFADMLLQHRMEVVVVPELPKGELPRKAK
                     AAKDWKEVRKKL"
     gene            26711..>27471
                     /locus_tag="PLS35_05055"
     CDS             26711..>27471
                     /locus_tag="PLS35_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNI18055.1"
                     /translation="MLLEGKKGLIIGVANKHSIAWAIAQAAAREGAQMLFSYQNERLR
                     ENVEELVQTLPGASACVCDVGDDSQIAAMMKEAAEKLGRLDFVVHSLAFAPREELTGE
                     FVNTTRQGFATALDVSAYSLVAVTRAAMPLMTDGGSVVTLTYLGSERVVPHYNVMGVA
                     KAALEATVRYLAHDLGPKNIRVNAVSAGPIKTLAARGVSGISKMVDHHRAFAPLRRAT
                     EQGEVGDTALFLVSPLGRGITGEVIYVDGGYHILGS"
BASE COUNT         5700 a         7244 c         8539 g         5988 t
ORIGIN      
        1 gcgcatcggc ggcattgcga tctttctggg gactgccaga gaccggtcga agggaaaaga
       61 tgtcgacggc attaccttcg agcattacga gggaatggcg cagaaaaaat tgcgggagat
      121 tcgtgaacgc gcgctgaagg atttcgggat catcgaactg tcgatcttgc accgatatgg
      181 cgagatcggc atcggcgata acattgtatt gattattgcc ggtgcggagc atcgagccga
      241 ggcctttcag gcctgtaagt gggccattga tgaattgaag caaatcaccc cgatctggaa
      301 actcgagcac actccggaag gcgaggtgtg ggtcgaggag cacccttagc ctatcccgac
      361 agcaataggg aaagaggccc gcgtcctcga catattatgg accacacgcc cgatacaccc
      421 gttcctacga tatacgaccg gctgggacgt ccgctccgca gcctgcgcct gtccgtgacg
      481 gaccgctgca atcttcggtg taagtattgt atgccggagg atgattacgc ctggttgccg
      541 cgcgatacca ttctgacctt cgaggagatg gcggagctca ctgcagtctt taccgagctc
      601 ggggtagaca aggttcgtct gacgggtggg gagccgctgt tgcggcggga cctgccccga
      661 tttgtccggc agctgtcgga aaatcgacgg atcacggaga ttgccctcac cagcaacggc
      721 gttctcatgg ctgatcaggc cgccgatctc tcgttcgccg gtctgaatcg cgtgaccatc
      781 agcctcgata ccctccgtgc ggaccgattc cgtactctga ccaagcggga tctccatcac
      841 caggtgtttg acggcattaa agcggtggtg caagccgggt ttccgtcgct gaagttcgac
      901 accgtcgtca tcaaggggta caacgacgac gaactcatcg acctgatcga atatggcaaa
      961 acggtcggcg gcgaagtccg attcattgaa tacatggatg tcggcggcgc caccgattgg
     1021 tccatgaagc aagtattgtc gaaggccgaa atgctcgaac tggttggccg ccactatggc
     1081 ggcgtcaagc cggtggttga gaacagcgct gcgccggcgg agcgttttgt gcttccggat
     1141 ggcaccatct tcggcatcat tccctcgacg accacaccct tttgccgtgc ctgtgatcgc
     1201 agccggttga cagccgacgg gatgtggtat ctctgtctct atgcgaaaga cgggctcgat
     1261 ctccgtgcac cgctccggca gggccgaacc cgggacgaat taaaagcgtt gatcatcgcg
     1321 gcctgggaag ggcgggcgga tcgtggcgcc gaagagcgca aagcgctgga ggtgttaggg
     1381 ttgcgtgagc agcgcctcat tgaaattaat cggctccgag aagatcccca cctggaaatg
     1441 catgcgcgtg ggggatgacc gccagtgtgt gctgctccca ctgccggatc cgctgatgac
     1501 cctctcctgg agcagtcgat gtttgatcct catacgctga ctatcctgag tcttggattc
     1561 gtgctggggc ttcgtcacgc cctggacgcg gatcaccttg ccgcgctttc cactgtgctg
     1621 gcagaacgtc ctaccgtaca ggcgtccacg gcgatcgggt ttttctgggg acttggccac
     1681 acgctgatgc tgttgtgcgt cgggaccctc ttgctggcgt tgaacctcac cattccggaa
     1741 tccatggcaa atctgttcga atttgccgtc gggggcatgc tcgtggcgct ggggctttcc
     1801 ttggcccgcc gggtctatcg tgagcagtgg catgtacaca cccatgagca tgggggccgg
     1861 ccgcacgtcc atctccacag tcaccacttg caggcggatc acacacacgg gcattggtat
     1921 cagggctcgt tgcggcctct ggcgatcggg atggcacatg gattggccgg ttcggcagcg
     1981 ttgatgttgg tcgtattgtc gacggtgacg gggatggccc aggggatcgg ctatattgtg
     2041 gtgttcggtg tcgggtccat tcttgggatg gtgggtgtcg gggcggtact cagtctgccg
     2101 gtgatctatt ctgtgacggt cggtcctcgt gcctactgga tggtgcaggg actcacctgt
     2161 cttgccagca tcggccttgg ggtggtgatg atggtccgaa tcgggttagc cggtggctgg
     2221 tcctagccgc ttggttcgtc gcagatcttc cgcgctcctg cccacactcc ctttttccat
     2281 gacacgcctc ggtaacgaat gacctatgta aatctgtgtg cgcctttttc agaggtatgt
     2341 tattctgccg cccagtgcgg aggactcgtc tacaatatcg taaaaacatg ctgacacgcc
     2401 ggggcacgtc cggagagcct cggctatgaa tgtacgtgta ttaggttgcc atggttcggg
     2461 gcagcttgtt ccgggtgaga atggtccggt tcagtgcggg acctgcggct ttctgatcaa
     2521 tgaggaattg ctagttgatg cgggaaccgt cggatcccgg ctctatctgg aagagcagcg
     2581 gcggatccgg ctggtcctct tgacccacct gcattttgat catattcgcg agttgccgac
     2641 actggccgat aatctggtcg gtgagatcga ggagccggtc gtcatcgcgg cgattcccga
     2701 agttttggac gggttgcgtc ggcacatttt taacggcacg gtctatccgg attttttccg
     2761 gttgcccgat cctgtgaagc cggtgtttgt gtcccgtgaa cttctcccgg ggcaagagac
     2821 ggtgttgtgc ggtctgggag tgacgccgat tccggtgaac catgtggtcc cgacggtggg
     2881 atttctgatc cgcgaacaga gacggacggt gctgtttagc ggtgatacct atcagacgga
     2941 cgcgctttgg caagcggccg gggatgtgga tgcattgcag gcggcattta tcgaatgttc
     3001 gttttccgac gagatggccg aattggcacg agtggcgaaa cacctcacgc cggcattgct
     3061 ggccgaggag tttgccaagc ttgcgaaacc ggaggtgccg gtctatgcct accatttgaa
     3121 gccgcggttt cgagcacaga tccggcagga actcggagga ctcggcattc gacatttgac
     3181 cgcgcttgaa gagggccaaa cccttgtact ataggtgagc agagatggct tggtttaaaa
     3241 aaggaaaaac tgaggaagca gagccaccca aacgctcgaa aacggcggag gggatgtggc
     3301 tgaagtgtaa tcattgtcgg gagatcgtgt atcgcaagga ggtcgatcgt aacaataagg
     3361 tctgccccaa gtgcgattat cactttccca tctcggtcat cgaacgcatc aatctgttgg
     3421 ttgatctcgg cacgttcaaa gagtgggatc ccgagctcga gccgcaggac ccgctgcagt
     3481 ttcaggacac ccgcagctat aaagaccgga ttaaggcaca gcaggagaag acgggtcgga
     3541 aggacgccat ggccatcggc cagggtgtca tcaacggacg aaaggtcgcc ctttgcgtgt
     3601 tcgatttcgg gtttatgggc ggcagtatgg gctctgtggt gggtgaaaaa atctgccgcg
     3661 cagtcgatcg cgccttggaa gcgcggatgc cattgatcct cgtgacggct tccggcggag
     3721 cccgtatgca ggaggggatc ctgtcgctga tgcaaatggc gaaaacgtcc gcagcggtcg
     3781 cgaaactggg cgaggccaaa ctgccgttta tctcgattct ggccgatccg accttcggcg
     3841 gcgtgacggc aagtatcgcg atgctcggag acgtgattat tgccgagccc aaagcgctga
     3901 tcggattcgc cggcccgcgc gtcattgagc agacgatcaa gcaacaattg ccggaccagt
     3961 tccagcgggc ggagtttttg ctcgaccatg ggatgatcga tatgatcgtc gagcgcaagc
     4021 agctgaaaga ggcggtcagc accctggtcg gtcatttcta acgggatgcg gacacagtct
     4081 gccggcaatg ttcccgctct gtccgctcgc gtgaggtacc agcaccgttc ggtctcgctc
     4141 cgtgttcgcc acggccttcc cggacgatca tttccgcatc ctggtgagct tcgacgtcaa
     4201 catgcaatgt gagtctcccg tggccccgat gacctatccc gctaccgtgc aatttttgta
     4261 tggcttgcaa ctgcacggga ttaaattagg tctcgaaacg attcgcacgc tgctcgcgcg
     4321 ggtaggcgat ccgcatcgtc agtaccccgt gttgcacatc ggcggaacaa acggcaaagg
     4381 ctcgactgcc gcgatgaccg cgtcgatttt gcaggtctcc ggccatcggg tgggactgta
     4441 tacctcgccg catttgatcg actttcgcga gcgcattcgc gtcaacggcg agatgattcc
     4501 cgagtcgcgc gtcatccatg gcgtcgaatt gttgcgcgcg gcttccgcgc cggatctggc
     4561 tccgacattt tttgagttca ccacggcgct cgcgtttcac catttcgcgg aatgtcaggt
     4621 ggatgtcgtt gtgctcgaag tgggcttggg aggacggttt gacgccacga atgtcgtgga
     4681 gccgctcgca gccgccatca ccacgatcgg actcgatcat gaggcctatc tgggctcgac
     4741 gattgaagct attgcgatgg aaaaagccgg gatcatcaag ccgggagtgc cggtagtgct
     4801 gggtcgaatc ggtgagccgg gttctcgagt aatcgaagag cgcgcctcga cagtaggcgc
     4861 tcccgtcctt cgtctcgatc gggacttccg ctgtgaagga tcctcgccgg ctgactgccg
     4921 atacacaggt ttcggactgc ggctggagca cctcagttgt cccttacagg gtcgatttca
     4981 gatcgacaat gtggcctgct ccctggcgct gatcgagctg gctcatgagc gcggtgtcac
     5041 agtgacggag acgtccgtac gagcagggct gcagcaggtg gcatgggagg ggcggctgga
     5101 gattgtcggc caggagccga cggtgatggt agacggggcc cacaatccgg cggctgcggc
     5161 ggtcctcgct gaatatctat ccgaatggaa gcgtgtcagc cccgccgcgc ggctctgtct
     5221 aatcgtggga atgatgcgtg ataagcatcc gcgcgaattt ctggcgccat tgctgccgtt
     5281 gatcgatacg ctgattctga ctcaggcgga ccttccgcgt gccgcgaccg ggtcggaact
     5341 tcgtctgttg cttgagcatg acgcaccgtt tgcgcaagtt gcgacgaccc cttcggatgc
     5401 cttggccatg gccaagcatt cagccgctgc gtccgacgtg gtttgtgtca cgggctcctt
     5461 gatgctggtc ggcgagatca aggcccttct tcgaggggat tccctctcgc ctgttcgtgg
     5521 ttgagatggg gcgagacggt tattcggttt caacgtggca gcgcctgatc agccactttc
     5581 cgtgcatcct ctccatttcc attcttttcg gtctctttcc gctcttcgcg ccctcggcgt
     5641 ggggggaatc gaccgtcacc accgtttcac cgacaaacca gcctctgacg gtcaccgctg
     5701 agcgcatcga tcatctccaa gagcgtgaag tgtatgaggc ggatggatcc gtcgtggtga
     5761 cccaagggaa tttacggctg accgccgatc atgtgacgat caatgccttg cctggcacgt
     5821 tgacggcaac gggccgtgtg catttctggg acgcgagttc cgatctcaaa tccgaacggc
     5881 tggaattgaa tgtgaatacc gaagccggca tcgtcgccaa cgggtcgatg tttatccggc
     5941 caagcaattc attttttacc ggccgcagta tccgaaggtt gtcggaggat cactaccgca
     6001 tcaaggaagg tacgtttacc aattgcgatg ccaaggaggg cgaagtgccg gcctggcgat
     6061 ttaccttcga ggatctcgat gtgaatgcgg gagagagcat cgggatgaag cgggcgtggc
     6121 tctgtgcgaa cgatacgccg atcattccgg taccaacact gacgtacccg ctgagcaacc
     6181 ggcatagcgg gtttctcatt ccgacgcccg gctacgataa ccggttcggc ttgcactatc
     6241 agcagggata cttctgggcg atcaacccca gccaggatct cactatcgcg ccgtcgtact
     6301 acaacaatct gggctacggc agcgatttca cctatcgcta ttatctggac cggcgatcga
     6361 gcgggcagtg gtttgccagt ttcctgcagc agaccaagtt gccgaatgtg tcgggagtgg
     6421 atcaggttag ttcggatgag cggcgtctgc gcggattaat cagcggccag catgtccagc
     6481 aggtgacgga cacgctcctg gtgcgcgggc aggcctcgtt cgtctcagac cggcaatacc
     6541 tccagcaatt gagcaattca ggtacgcaac gggcctcgcc cagcggtgaa tcgacgttgc
     6601 tggccacgca gcggctaccg tacggaagcg cctattttct cggacaatat ctgcagccgt
     6661 tgaattccgg cggcccagac acctttcagc gccttcccga ggtggggtac gtcctgccga
     6721 acacatcgcc attcgggatg ccggttctcc tgaacctgga cacgaacttt gtgaacttct
     6781 atcgcgaaca gggtttcgcg gtgaatcgca tggacttcat gccgggggta accacagagg
     6841 tgatggacgt ggggcatgtg attggcttga cgccgcatgt caaatttaag gaggtctatt
     6901 acacgcgcgg gattcaggag gccagtccgc tgcatcgcga gacattctgg gccgccatgg
     6961 atgcgacctc gaagttgagt cggcggtata caggcgggga aggaggaagt ttccttcaca
     7021 cgatcgaacc aagcgtgatt tatgaatacg tcccgggcag cgatcaaagc aagattgcgc
     7081 agatcgatca gatcgacgac attccgaaga aaaatttgct gacctatgcg ctgcgtacga
     7141 agttcctgga gcaacagctc aatgggcaga gtttcaattg gttggatctg accgtggcgc
     7201 aaagttatca cgtaggagcg gtgcagacgc gggcgcgaga atttactccc ggcgtattgc
     7261 cgttcctggg gtcgctgaca cagcctttgc agccggcgac ggtagatgtg cagggccgga
     7321 agatgtccga tgtgtggatg cgtgcggtga tcgggaacac ggcaccggaa ttttttcgtc
     7381 ctcccgctat taacgaagca ctcggaaaga atgtgacagg cggacaaatg ttgccgccga
     7441 caaattccta tctacaggtg gatgcgtttt tggacccgta tcgggggacg ttcagccagt
     7501 ggaataccga cctgcgagtg cagcaatcca attactggta cgttgaggtc gggcaacggt
     7561 tcagtcatga cggcaatcga gtccgccgcg gagatatttg gaatccggtt tccttcaatg
     7621 aagtctatgc ccccaccaag gaaattcaat tcgccacggc cggcggcgga ttccggacgc
     7681 cctgggggtg gaccatcggc gccaagggat attacgatgt gaaaggcgga cgcagcccgg
     7741 aatttgatgt ggtggcgctc tatcaaaatc catgtaagtg ctggtcgctc gggctgtact
     7801 acctgcagtt cccggaccga cagagttatt ccttcatgtt cagtatgacc ggcattggct
     7861 ggacggaaaa tttcggtacg attgtggcca ggaccatcct cggtccgttg ctggtaggag
     7921 atcgtgggtt gccctgggcc gctcccggtg gtccctatgg tcgtgtacaa aatgcgctcc
     7981 ctgttcagtc gatgccaatg gggcgctatt agtgaagcgc ccggttggat ttccatctct
     8041 caggagcatc gcccatgatc gaaacgaagc gtgcagctgg aattcgactc aaccggcgta
     8101 ccgttctgac gacctgtctt ctgatgctag ccatcgccgt gcaggaaatt tacccgtcag
     8161 ggctttctgc cacagagcac atggcacatc tcgaacgggc cgcggtgttt ttgaaggcgg
     8221 gagattatcg acaagcgctg gaggcgtgtc aggcagaagt gaaggatagg ccgtccgctc
     8281 ggagttatct gtatctgacc tatgcctatc atgctcttga tggctacttg cagtatttag
     8341 caaacaccga tcaatgggtt cgagtcgaac atctctatgt gaatctgtct acaggacgcg
     8401 tggaggattt gctcgatccg ccggatgtcc tggctcggat agccaaagag gtgattcagc
     8461 aggctgcgca acggcaggcg gatgtcaccg cagctctagc cgggcgcttg gacgagactc
     8521 tggtcaaaca actgtggcag cagcaaaccg catggagaca ggcccgaccg gatgattggt
     8581 ggtcggctgc accgccgcag tggcaatggg cggcaccgtg atttgactct ccgcaaaacc
     8641 atgttatagg atgacgaaca atgcctgaag ccgcttcagg acgtgaacca gacagcagtt
     8701 tgtgagtaga gaaggaggca attgtggcag gcgatgcgtt gaaagcgact gatgcaacgt
     8761 gggatgaaga tgtcatgaag gcatccgaat tggtcatggt agatttttgg gcggtgtggt
     8821 gtggcccctg tcagatggtc gctccgatcg tcgatgaatt ggcgacagaa tatgctggta
     8881 aagtaaaggt tcgtaaactc aatactgatg aaaacccgga aatcgccggc cgttatcagg
     8941 tcatgagcat tccgaccatc ttgttcttca agaatggtca ggccgtagaa cggctggtcg
     9001 gtgcgcgtcc caaacgccag ttcaaagaga tcatcgatac gctccttgct caacattccg
     9061 gcgccgccta gcgccagact ctcagcggca tgctgaccga gctgcgcatc tcgaatttcg
     9121 gtgtgatcga gcagctggcc gtcacctttg gcgccggttt cattgtattt accggtgaaa
     9181 ccggcgccgg aaaatctctt ctcatcgatg ctgtgacgct cctcgtcgga ggccgtgctt
     9241 ccgcagaaca cattcgtgcg caggccgaag aagcagagct ggaagcggcc tttcatctgc
     9301 caccagaaca tccgctcctt gcattgttgc gcaacaaagg ctttgcacgg tccggcgaga
     9361 ccgatattct cattcggcgc atcatctcac gcacaggtcg caatcgaacc tatctaaacg
     9421 gcaacctgtg ccctgtccat ctcctggaag aactgggggg cgccctcgtg gatgtgcacg
     9481 gacaacacga gcagcaatcc ttgttgtcac cttccgctca gatggatgcg ctggatgcgt
     9541 tcggcggctt tcagccgctc cggcaggaat atcagcatgc gtatcgggat tggcaggacc
     9601 gggtgggtga tctggaaacg ctcactgctc agattgcaca gcgtcgggag cgggaggacc
     9661 tcctcagatt tcaatttcag gaaatgacgg acgcggggat tgaggcaggc gaagatgcac
     9721 gactcgaaca ggagcgtcca cggctgatgc acagccagca gctcggagaa ttgtccgacc
     9781 agctccatga gctgctctac gcgggtgacc agggagtctt aagtctattg gctggtgcca
     9841 ggaagctgct tgccaaaatg gagtcgctcg acgccacgac tgtcgagtgg atcaagacga
     9901 tggatgaagc ggtcgtcccg ttaaaagatc ttgctgacca ggtccgccgc taccgtgatc
     9961 atgtggaggc caatcccagc cgattgctgg agatcgagca gcggctggat cggttgcatc
    10021 ggctcgcgaa aaaatacggc ggttcgttgg atgcgatgct ggcttggcag gattctctgc
    10081 gtgcacagct ggctgaatta gaccatgcgg agacccatct gcaggaacgt actcgtgcgg
    10141 tcgagcaggc tgtactcagc ctacgcgatg tggcggaccg tctatctcgt aagcggctcg
    10201 acgcggccaa aaaaataacg gcccaggtga tgaaggaact gggggggctc cgaatggagc
    10261 ggactcgttt cagcgtggag gtctcgcgtc tgtccggcga cgcgccctac ggtcagacag
    10321 gacaggacac ggtggagttt atgttctccg caaatccagg ggaaccgctc aaaccgctgg
    10381 caaaggtggc ttcgggcgga gaattgtcgc gtgtcatgtt ggcattaaaa acgattttgg
    10441 ccgagagcga tcaggtgcct gtcctgattt ttgatgaagt cgatgccggt gtcgggggcg
    10501 ccgttgccga ggtaatgggg gtgagactga ggagtctcag tcggcaccat caggtgttgt
    10561 gcgttaccca tcttcctcag gtgggctctc aagcgcatgc gcattatctc gtggaaaaat
    10621 gggttcggca gaatcgaacc atgacacaag tccgcctgct ggacctggat gaacgggaag
    10681 aagaagtggc gcggatgttg gccggcgtga cggtgaccaa gaaggcgcgc gcggcggcgg
    10741 ctgaaatgat tgcgagcgcg aaggaccggc agtaacggcc ggttagttcg tgggcgaggt
    10801 tttggcgatc gcgagtgccg gcagggaaga cttacattct cgcaccacgt gcgcgaagag
    10861 ctcggtcaac ttgggatcga agtaggtatc ggcttgcagc agaatctgtc gaatggcttc
    10921 atcgatggat gtcggctggt gtcccggcat atccaccgtc aggttgtcga acgcctgggc
    10981 gatggcgaca atccgtgcca aaagcgggat gccttcgcct tctagaccgt gtggattgcc
    11041 cacaccgtcg aagcgctcct gatgatacgc cacgatctgt ccgacctccg cttggaatcc
    11101 aagtggcagc agcatccggg cccctgtctc aaagcactct ttcccatgag tgttcgtctt
    11161 caggctctca tcatcatccg ctgaatactt gtagttggag aggctgatct tgccgatatc
    11221 atgtagaaat gctcccagcg ccagagcctt ttggtcgggc aggggcagcg tgagctggtt
    11281 ggcgagaagt gaggcataga aactcacgcg gctgcaatgg ttgagcagtt gccggtcctt
    11341 ggcttcgagc aggtcggata acagtgggat gagcgctgtg acgtccgctg cagttccggt
    11401 cgcgtccgtt gaggaattgc cgatcgcgaa gtacttcgat tgcagatctt tccacgcttc
    11461 tgccgcttcc tgttcggtgc cccacaaggc cgtcgatgtt ttcaggaacg atcgtaagta
    11521 gtccagtcgc tgtttctttt ccagggtctg gttgatcagg gtgatcagct ccgtgacgtt
    11581 gaatggcttg agcaggtagc cggccgcgcc gtgccggatg ccttccatgg ccgatttcaa
    11641 gctgccatag cctgtaatga tgatgacttc tatgtctgcg taatcctgct tgatgtcctg
    11701 caggaggtcg ataccttgac ggtcgggtag tttctgatcc agggtgatga gatcgatgtg
    11761 ctggtccttc agaacccgga gcgcggattt cgcattgtcg gcagagtgga tcgtgaagaa
    11821 ggggcgaaga ataactttca gcgcgtctcg cgggcctgtt tcgtcgtcaa cgaccaggac
    11881 cgacggtttt atgctgatct gttcagttga tgcctgagtt cccatccaga gcgctcccgg
    11941 tgcattgcga acttcaggaa agataaaacg caaaacctgt accgtctacg tataccgtaa
    12001 acggtcaata tatagcaggt ctaactattt caggccacaa gatatttgag cggggcgacc
    12061 ggcacgtgaa aaagtatgag acacatgccg agactcttgt gccagtctgt gtcattcctg
    12121 aggcagtgaa gcctcggcat ttcccccgcc gctatcaggt cggctgatgc ccaatgtgtc
    12181 catgcgatat ttcagcatgc gacggctaat gcccagcatc gtggccgcat gagtttgtac
    12241 gtatgtcgtc cgcttgagcg catctaaaat aatttcacgc tcgaattcca tgacagcctt
    12301 ttccagggat aatcgtccgg cgagggtgtc atcccgaagg gacgatgagc gcgtatcagt
    12361 ccggagaata ttcgggagat gctccggggt gatcaccgtg gagcccttcg accagatgaa
    12421 ggcttgctcg atgatatttt ccatctcgcg cacgttgcct ggccacggat aatgtgaaat
    12481 caattcgagg gcgtctttgg tgaattcctg agacggtcgt ttctcttcct caatgcgttt
    12541 cgaaagaaaa tgcttcgcga gaagggcaat gtcctcgccg cgctcacgta atggcggaag
    12601 gtacagcgca atcacgttga tacggtaata caagtcctcg cggaactggt ttttcctgac
    12661 catctcatca aggttcttgt tggtggccgc gacgatccgg acatcgacct tgatggattg
    12721 caccccgccg acccgcgtaa attcccgttc ctgcagtacc cgcaagagct tggcttgtgt
    12781 tgcgggactg agatcgccga tttcatccag gaacagcgtg cccgtatgcg ctaattcgaa
    12841 ctgtcccact cgtcgtgccg tggcatcggt gaacgaaccc ttttcatggc cgaacagctc
    12901 gctttcgatg agcgtttcag gcaacgccgc gcaattgagg gcgacgaacg ggcgttcccg
    12961 cctagcgctg ttgtaatgga tggctttggc gaccagttct tttccagtcc cgctttcacc
    13021 ggtgaccagg acggtggtgc ggctatctgc cacttgttcg attttggcgt agatttcctg
    13081 catcgcgggg cttttgccga tgaggttgtg gaaggcatag cgctgcacaa cctgagcgcg
    13141 caattgccgg acctcttgtt ccagttcctg cttacccaag gttcgttcga tgacaatttg
    13201 aagttcctcg acgtcgaacg gtttggatag atagtcggcg gctccgagct tcatcgcatc
    13261 gacagcggtt ttcactgcct tggtgccggt cagcataatc accggtacgg ctcgattctc
    13321 ggttcgcatg gtctgaagcg cggacagacc gtccgctccc ggtaggatga cgtccaggag
    13381 gaccaggtcc ggggcatcgt gtttaaaact gcgaaggccc tcagcagcat ccgccgcttc
    13441 aaaaatttca tacgtgggtt ctaggaccgt tctcagcgag gcccggacac gcggctcatc
    13501 gtcgacaagt aggactcgtt ttttcatatt actcccgtgc tgctgagact gtgggcgctg
    13561 aatcgggaac aggaagattc acggagaacg tggagcccgc tcctaggcgg ctttcgacta
    13621 tcagggtgcc accgtgctct cgaacaattt gatgcgcggt ggtgagcccg aggccggctc
    13681 cttcactggc actgttggaa tgtttggtgc tgaaaaatgg atcgaaaatg tgctcgagat
    13741 gatcgggcgc gataccacat ccttcatcct cgacctgcaa ttgtacccat ggggggcggc
    13801 cggcttcgga caggagtcgc gtatgaaccc gaatgatgcc gttgccattg ggcattgcat
    13861 ccagagcatt caggagcaga ttcaacaaca cctgtttgat ttgctggcga tcgagcaaga
    13921 gcagaggaag ctgcggcgac aggtgcgtct gaacctgaat tccccgatgc gcggcgctcg
    13981 atgagatgaa gccgatacaa gaggcgacga gatcgttcag gtccacttcc gtggggcgcg
    14041 gcgtcatgta actggcatag tcgagaatct catgaagcag tcgttcgatg cgatggatgt
    14101 cttcgatagc cagtttgctg aattctttga tgaagacggg atcctgctgt cgctgggggg
    14161 ccagctggac gaatgttttg atggacgtga gcgggtttcg aatttcatgg gcaaacccgc
    14221 cggccatgat ttcgagagaa cggagtcgat ccgtccgtct catcaatgtg gatgagcggc
    14281 ggaggtcgtc ttgcgtcagg taatgatcga ggctattgcc tgcgatgtgg gtaatcgcgt
    14341 caacgatggc gagcagttct tcgccgacct gcgctttcgt gtcgagaggc cccaggatgc
    14401 agatcccgag cagtcgccct ttggttcgta gtccgaggca caggctcgcg cgggccgtcg
    14461 ccatcgccaa cccaagcggt ttagagggat tctcgctccc atgacatacg tagggtttgg
    14521 cggagagacc tgcaacgagc gggtgatcac ccgtcaaagt cgatggtaat gaagagggat
    14581 ggttgtgtcc atacgatgca aggagacgat accgttcacc tgtagactcg cgaatccaca
    14641 gggctacttc gggcaaccga gtgtattgcg aaagagccgt cacaatcgaa tgtccgagac
    14701 atgacaggtc tcgtgccgat cccagggttt cagcgaagtc acggatcagt ctgacaatgt
    14761 ccgtggaagc tgaaaggacg ggcatctgcc ggatgttggc gtggagcgtc atagtcccgt
    14821 gggaggaata tcattccgga caccaggttt ccccaggtgt cgcctgactc actgcatgtt
    14881 ttatgctagt gagtgaagta tatatgcgct ctattgtttc agctagagga atccggtttt
    14941 tcagcggcgg cgagcagaat ctgtccggcg agagtacgaa caagtctagg tgtaagcggt
    15001 gtgaagcgtg cgatcagtgt ggtcagactc tcttcctcaa acgtgtgtaa ttgccctgta
    15061 cgtagtgcta ccgcgcactg tcccatacgg tgcaaggcca agcgaggcgc tcctgaaaat
    15121 gcgtcgatgc cgatttcgtg taaggccggt ctgtacaggc gagccagatc tgtggcgggg
    15181 gtaaaagcct tcaggataag tttcccgccg ggccgaagca cgcggaagaa ttctcggagc
    15241 gagtgcacgg gtgagcgtac aaagctcaac gagaaatttg ccaccacacg atcgaatgag
    15301 cagtccgcaa atggcagcgc gtctgccgag cgaccgagga tccattgctg ggtgacgggc
    15361 gtcggtgcag agatccgtcc tgaaaagagc tcgtcaaggt gttgcagcgc ttcttttgtc
    15421 gaggctcggg cagcatgcag tgaagcagag gaaaaatcca gcccgacata tcggagtggg
    15481 tggttatgat gccaggtttg agcgcgcagg cgatatgaaa gattgagtag cagcaaccga
    15541 gcgaaggaat gcatgccgca tccgatatcc aggagggcga cattgccgtc gagaggctgc
    15601 aagagttgat agagttgatc gatagactgc caatgtgccg gcagttcatc cagggcccgg
    15661 gtgagttccg tgtgtgccgt ccaaagtcgt tcgttactcg tcgtgccgat ggcgtgttta
    15721 tgacgaagct gatcgcgttc gaaccgggca cgtgtggatg ttgcaagcaa gagcgacgga
    15781 agcatgaccc ttcctggttc gtgaagtgcc tgtcccgctt ggcagaggtg tgacacctgt
    15841 tgccaacttg cgcgtagagt cgaaggtggc tgagggcctg atgtcaaggt agacgacggc
    15901 aacagtactt tcctgccttt aacgccaagc cgggagagga ggtcattcac gagaggttga
    15961 ggaggaggcg ggatcgagca gtcagggcca tcttgtccgc cggtgaggac gactacctcc
    16021 gtttcacagt gggccagttc attgtgcagg accctgacat ccgtatactg cgcggcaact
    16081 gcgtcggcca aaaaatggtc gatgtcgagc ggaatcccga gtaaatttcc caggcccagg
    16141 cacgtcccgt ttaggtgttc ctgtaacaga tctcggtggt ggagggtttg caggcactgt
    16201 ccgaggtcga gtgtcggatt gagaagcaga aggcgccgaa tccgcctgtg ccaatcctgt
    16261 ctgcgcaatg cgattcgagc gggaaggtcc ggcgccaata tcgtcagggg tctttccggc
    16321 cagtgttcct gcacgaaggt caacactgta tcaaggtcgt cctcaagaga ggtgaatgtg
    16381 gtttgcgaag ggtctccgtc gctgaggcca atatggcggc tgtgatcgta acgcaccaca
    16441 tgaaagccgc ggccagccag catataagcc aacgcgacat aggcgtgctg cgtcgtgccg
    16501 tatccggggc acaggagtac cagcggatca tgctcgctgc cgcctcctgt agggaagtca
    16561 tgccgcaacg caatcgtata gccttcacgg ttccgcaccg tgtgtgacac ggtgagaaca
    16621 ggggcatccg acggggtttc ggccagatgc tgctctgccc ggaattcagc ctccatgatg
    16681 ctacgcagtc tctgctcttg tgcgggggac agagtatcaa agcgtatacc gatctgctgg
    16741 aggtgatgtc ccgttgctgc gaggcggagt ttcagcctcc tttccgccga atcgttccag
    16801 agaatctgtg ccgtcagtgg ctgttcggcc tctggtgctt gggaggtgcc gggcaccatg
    16861 gtgatcgact ccagagggtt cagatggatg acccgtgagt gttcggtcaa gacgacctct
    16921 gtcggcagtt cgatgcaagc cccgttcaga ttgagattga tgatcttgcc gaacggacgc
    16981 gatgaggccg cgtcggatcc agttatgtat acggggcaag acacgctcag tcgagcggct
    17041 tcccgccggt cttccggcat ctctcgaagt ggaaggaggt tccggtgctg gatcgtttgg
    17101 gctatgggca gggggagaat cagtccttcg aatgtgatct gcggggaccc ctcctgcagg
    17161 ccctcgagca aggatctgag tacattgcgc tcatgttcga cggcgagagc aaaggaaatc
    17221 acaaaatcct taatggccgg caacgacaat tcggtggatg cggagggccg ctcatgcacg
    17281 actccttgca gttcaaggaa gctgaccggg gtcttgatca cgacataggc gggctgcaga
    17341 tggatcgaac tttccattcc tcggatcgta aggagcgccc cttccgtact catattgcgt
    17401 aacagcccgc gtagggtcgt gcctccgatc gacaattgaa cagggaggga aaacggcttg
    17461 cgctctgacc ggcgacgttc agggcgttgc gaggtgtctc tcccaggctt gtgaatgtcg
    17521 gttgggggcg gcgagattgt tgttgccggt gttccagtca gggagaagtc ttccgaggcg
    17581 gggatgcgaa ctgtgatggc taatccggat tctggtgtgt gttcgacttc gatggcaccc
    17641 cggtgtacct gaacgatgcg atgtgcctgg aataaactca ctgccgagcg atcgtggcct
    17701 gattggctgt ccggaggcag acggagtgag ctggcacttc cccggatcgt aaagctcgca
    17761 tacgtacgaa gcggccggga aagcgatgcc acctcctgcc caggacttcc tgaagtctcc
    17821 acggcgagta ctgctgtgtg caaggttaag ctgggacgtg tgttgtcagg tgtggatcgg
    17881 caggcatagc tgagcaagat atgggtgagg tcttccaggt ccgcacgcga accgagaatg
    17941 agtggaaggt tcgccgcaaa ctgagtggtg aggagaaatt gggaatcttc cgtctcaccg
    18001 tgcgtcgtct gggcaactgt ctggagaacc tcattcagat caagaagaat caattctgtc
    18061 ggcggggcat ggatcgcttc ggccaattga tgtcccagct ggctcgcttg cgaagcctga
    18121 gcgaccatcg tttcaacatg ttgctcgaac cggctgtgtt ggatcgacgt cagcaatcgt
    18181 tgtgagtcct gaagaaggga cgtaaggggt tgctgcagtt ggcgggagac ccgggctgcc
    18241 tcgcgggaga gggtgagttg acgatcggtc tcctggagac gagactccag ttccgccaac
    18301 cggttctgtt gggccttttg ctgggataga tcccgcagca atccgaccgt tcccaagtat
    18361 cgattcgtgg agtcgaaaag gcctttcgct gtgacttcga cgatgatggg tacggatgga
    18421 gcctcgggga gggctttgcg gtagagcgtg agttccaccc ggcgtgtcga acggctgcct
    18481 gcccggcgct cattcaatcg aaaacgcgcg gttggttcct gttgaggagg aagcaggaga
    18541 gagtagtgcc gaccggcgag ttcctcagcc gtgtatccaa gcattgcggt gacggttgga
    18601 ctcacataca cgaaacgacc gtccgcatcg agttcgtaca agagatcact caacgtttcg
    18661 atcacgcgga ggtggcgttg aaacgtatgg tccagcttca tttgcaggtc gcgcttttcg
    18721 agggcttcct gtacggaaaa gaggagctcc gtgagaaatc cgggcgatcg tttgaagagc
    18781 aaaaagtcgg ctccgtgctg gagggcttga acggccgtgt gtgagtcact ctcgttcgtc
    18841 tgaagaagga ttgccgcata gggcgcattg cgacgaaggc gcgcgagcag atcgagacct
    18901 cgttccggac tcagatcctg atcaatgaga atgatttgcc agtcgccctg gcgggtgaat
    18961 gcaagggcat cttcggacga gtacccggct tcgatgcgac agcccacaaa aaagctccgc
    19021 aagctggtgg tcaccaactt gatttcgtcg gcattctcat tgatgaggag gatatgcagc
    19081 gagggttcgg gcaaggacat ggaagatctc atggtcttca tgcaggggcg cgttcagcgt
    19141 gccccgaggc tgtgagtgcc ccggggaatg caccaccgta cagtgatgaa catgctctcc
    19201 ctctcaggcg gacaaatctg tccttcccca gctgattgga ctgcatgatt ggttcaatat
    19261 catacacctt cttgagaatc ccagtccgcc gcccgagggg gcggttccac ttcggtaggg
    19321 gaggaacgat tgaaatctga gcgactgacg aaagctaggg agaccctgaa ttggagaagt
    19381 gttccgattg gaacaggttt tagtatgagt cggtgcggag cctggattgg gctgaaccca
    19441 ccacagccgc gtcctgtggt gggtgcctga cagtgttggc tagtgggccg cggccgtggt
    19501 cgacgactgc tggcaggcgc caggcgtcat atagagcaga tagttaccca tgccatgttg
    19561 gatgttaggg ttttgcagaa gatgatggtg aaccatcacc atttcgtagt catcctgctt
    19621 cgtcaccgaa aatcctttgc tatcccgata aacctgatgc ggtcgaaagt cccggaatgt
    19681 accgtcggca tcgacctcgg ggacggtgcg tagcagggtc tcttttgaag tcttattctc
    19741 gagcgccacc atgagaagtt cgtcatgccc atggggatag gcaaatttga cgcagccatc
    19801 catgttgaat ttcagcggcg ccgaaaggac cgagactccc gacttgattt cgatcccttc
    19861 gtccgtctgg tccgggcccc gttgggcgaa cttgctgtag cacacaatgt tcacgcctac
    19921 ctggtagacg tccatctcct gcaccttcgc ccctttggga gcgacgtaca tggtgaagcg
    19981 cgccatcacg tctttcgtcg gcggggcctt gtgataaaag gccacaacgg acatgagatt
    20041 ttggcctttg cccaacttta ctccatatcc gtccgggaaa cgggcttccg tcatctccag
    20101 tccggccccc gcaaaaaaca acggctcgcc atcgcatgag acgctgggtt tgtcattgtt
    20161 caacatgagg atgtgatgga gataattttt cggaaggggt ttgccatctt tcgtgaacac
    20221 ctcggatttg tagccgatca tgtaggtgtc ctctggcagt tggaacacct tcttcggcat
    20281 gctggaggct agatcgccat catggccggt cggcagatcg acggggccga acgtgagcgt
    20341 gacggtctgc tcatcgatcg tcagtttcgt ctcccccaaa ctcggcatgg taggaatagc
    20401 gtgatgatca tgtccgccgt cggcatacgc caaactcgtg acgcccaaga acatgactac
    20461 taacagaagc gcacgattca taggcccctc ctcgttgtaa cagaccggcc gtccatgagt
    20521 gtgtgtaaag cgttgtaggg cgtgaccgcc tgtccgttaa ggagctgtcg gaggcggggt
    20581 gagtcgtttc cacaggtagc tgccgccatg ttgccggggc gggatattgt ctctggtgcc
    20641 gacacgcaca taccaccaga tgcctttggc ttccgtgcca tcctctgaga gaacgacttc
    20701 aaaggcaccc tcgcgatcgt ttcccgtttg tttccacgtg ccctgccagc gtcgatcgtt
    20761 gaagctggtg gtggaaaact gtccgccttg ccacgtgtag ggaccggtgc cctgggcatt
    20821 cagcgtggcg acgtatttct tattatcttc gatttccgtg acttcccatt caccacttaa
    20881 gtcgacgccg ttcgggacgg ccggctgcgc atccgaggcg cgatgaagca gtgccgcgcc
    20941 gttggccgcg gtctgttccg ttccgctgtc atgatggcgt gtcgattcat gccaggccac
    21001 cagaatccac cggccgttcc gttgctccag cactccggtt tcacgcaggt ggagcaccat
    21061 gcgttgggcg agatccgcgg gatcgctgta gcggatgtag tctaattcca ttgcgaacca
    21121 ggccacatct ttgttgttcc acatgcgcag atcggtgatc ggaatttcca gtttgaccac
    21181 cgaatcaaac tcgctacgca tatcggccgc cagatccgac catccgatgt atttgcgtcc
    21241 gtcaatcgtg tacccgatgg catccacgtc gtgtgacata tatttggaca tggtggccag
    21301 atcacgttcg gcattggcct gaacaagcct ccgcagggtg gtctccgcat cgtggggttc
    21361 cccggctcgc gccgtcggaa ggtacagcat tcccaagagc ggaccaagca ggagccaggt
    21421 tcggactcgc agactgagtg gacgaaagta atgcgattcc atgttcatta gaagtgagct
    21481 atggaggcct ctgaggcctt acctcgtgga tgtatggttt tgaggctacc tggtgccttg
    21541 aggcactgtc aatggatatt tccctgatcg atgagactgg tcatggcgca ggccacgacg
    21601 gtgatgttgt cgatgccgcc ggtatcaatc gccgcttgga tcagggcctg ggtcgtgagg
    21661 gagggactgt ctctatgcga ctgtgcaatc tccactatgc gttcgtccgg cagcattttg
    21721 gtgagcccgt cggaacagag caacagcaca tcgccgacga agacgtcgac aggaaagaga
    21781 tcgatctgca ggactggttc aagcccgatc gcacgggtca gcacgtggcg gtccgggtgg
    21841 cgggcagcct gctcaggcgt gaggcggccc tcgcgcacat agtcgtcaat gagggtgtgg
    21901 tctcgggtca actgcgtgat gcgtcccgcc cggacgagat acgcccgact gtcgccgaca
    21961 ttgagcacaa ggagtcgagg ggcgggcaga ggcatcagat gggccatcac cagcgtcgtt
    22021 cccatcccgt agacagaggg gtgacgatca gcataggcgc gcacggcatc atgagcctgt
    22081 tccgccatag ttgtgaggcg aaaagactgc tgctcggtat ggtcctcgtc gaggttgtcg
    22141 gaattgcgaa tgaggcttgg tgaaaaatcg gcgagcgtgt ccatgacgat ccggctggcc
    22201 acgtctcctc cgggatgtcc tcccatgcca tcagcgactg cccacaggcg gcgccggttg
    22261 tcgacgagaa aggcatcctg attggtcgag cggaccagtc cacggtccgt ctgcccgtag
    22321 cctgtccagt cccacttgcc ttgagcgagc gccacgttat ccgaccagac cgagtgactg
    22381 aggcccgtat cgcaagacca gccgttcgta caggcaggct gcgaggggct ccaggttttg
    22441 agtgtcggct gcattataac ggaccagctg atccagggcc tcgtcgtgac ctgcgcggta
    22501 atggtgccac aatcgaacgg cttccaatcc atcgagctgc agcaggtctg attcccgtgc
    22561 aatgtccaac tctcgttcga tggacttcaa gccgccctgc aagccgacgc gtcgggcggc
    22621 aaaacagaga tcgaaatgag ggatcgccac acgcaagccg tgaaagcagg tttgcagata
    22681 ggggatatcg aagaccgttc caaaaaatgt gatgagcagg tcggcttgat cgagttcctc
    22741 ttgaatggcc tctagagtaa gatcctggcc atggatcaac gtccgcatgc gcccctgacg
    22801 atgaagtccg acgatggtca cctgtccctc gcgggccgag agtcctgtgg tttcaatatc
    22861 gaggtagagc gcgcgggccc cgaaggtctg aaacaatcgc caatgatccc gggtgtggag
    22921 gcaatctgcg aaataccggg ggttatgcag gtcgaggtgg tcctgtgcaa gggccagctc
    22981 ggcgtcgtag gcagctttac gcgccggcgc gataccggtc aggtgggctt cccgccggaa
    23041 ggtcgcccaa tcggcgatgc cgctttccca cagacgtcgc tcggtcgatg gcccgatacc
    23101 ttttaaatgg acaaacgtgg atgtcagcat agaagcggcc gggattgtaa ccttgatttc
    23161 aggggataaa caagttagag tccgcgactg gtgcggagtt tcgtcatttg gtgttgcgca
    23221 cagtatcacg gtgtgaatga gggatcatga ccacgaacgg cacgatgaaa cgaattcgaa
    23281 tcaaggtcgg cggcctccaa ctggaggccg agttgaaggg gacgcgaacg gccgaggaac
    23341 tgtatcgagc cttgccggcg gaagggccgt tgaatgtgtg gggcgaagag ttctatttta
    23401 aaattcccgg cgtgaaagat catcgcgaaa cggcgacgac gcaagtcaaa atcggcgatg
    23461 tggcattctg gggagccggc caggtcctgg cgatcttttt tggccgcacg ccgatgagca
    23521 tgggcgccga ccctgtgcct gccgaccggg tgaacgttgt ggggcgggtg ttgggtgatg
    23581 cggcgacatt acggcaggcc atgaacgcca gtatgatccg ggtggaacga atcgagccga
    23641 cacaaccggt cgggtagggt tcagcgatga aggcgaacgg ggagcgattg ccatgagatt
    23701 ggcgaaagag cgcgtgcatc atatggctga ttcggtagtc gcacgactgc aggagttgaa
    23761 ttttctcgag gtgaccggag atcgaaaggc tcttcgcgaa gtgttggaac acacgatgac
    23821 ggaagagctg atggtcgaag accgcctcaa tgccgaagtg cgtcggatga tgcagccata
    23881 tgaacgccag atcgagcaag ggcaggtcga ctatcaaaag atgttcacca tgatcaaaca
    23941 gaaactggtg cgtgagcgcg ggatcatctt atgagtggcc gtgccactcg agacgggagc
    24001 cgtaatcgtg atcagtgatg acaaagccag ccaccttgcc cacctgatac tgggcacgtt
    24061 aaagaaaagc cctcagtgca ggctgaccga agacgacggc aaggtcttga gagagatcaa
    24121 acgcgtggtc gctgctgaac tcgccctgga cgcggagatt gataaaaaag tgcgggcgaa
    24181 actggcgtcc tacacgaagc agatcgtgga gggcagtccc gaatgggacg tcatgtatcg
    24241 aaaaacgtcc gaagaagaac agaaaaaacg cgtcaagccc taatcacgct ggcgaatgat
    24301 atgaacgagg tggcgcgatg agagtgagtc gctggatagc cctggtagtg gtggtgctgt
    24361 cgtgttcggc ctgtgctcat gaaaagccga aaccgctcgt gcctctcgag ctggcctacg
    24421 gggcgaaggc ggatgcgaat gcgctgaata atcagggcag gcaggccttt ctggccggcc
    24481 aaatggctga ggccaaggac ttcttcggac aggtgatcaa gatagcgccg gactccggac
    24541 aggcgcatta caattacggg ttgacgttga atgccttggg gcaaactgat caggcccgtc
    24601 aggaattcat cgaggcggca aatctggccc ccggggacaa ggtcatctgg gattctccgg
    24661 cattgcgtcc ctttggaaat ccggaagtgc ccaaggggcc ggcgagagag catccctacg
    24721 gaacgagcag gcctacgatc ggtagcggcc cgcggtaacg caaacgatgg tggagaagga
    24781 gagacgcgta gcatggcaag tggagtagaa gtcggggcaa aggcgccgga gttttccctt
    24841 ccggatcagg acggcaacct cgtgacgctc aagagtttga agggcaaaca agtcgtgctc
    24901 tatttttatc ccaaagacga tacctccggt tgcacgaaag aggcctgcga ttttcgggat
    24961 tccctggcgg cgatcaagaa gaccggagca gtggtgatcg gggtcagcaa ggacggcaaa
    25021 acttctcacc agaagtttat cgccaagtac ggattgccgt ttgcgctgtt gagcgatgaa
    25081 gatacggcgg tcagcacagc ctatgacgtc tataaagaaa aaagcatgta cggccggaaa
    25141 tacatgggca ttgagcgcag tacgtttgtg atcgatgcaa cgggtcgaat caaggcctcc
    25201 ttcaggaagg tcaaggtgcc cggtcatgtg gaagaggtct tggccgcgtt gaaggcctag
    25261 cttctgttcg gaccacagcg attgcgtcgt tgactcctct gtatcttcta tggatccgct
    25321 ggaaaaacca tcccatgctc tccgtgcagc tgcgttgtct ctgatgccgg gacttggaca
    25381 cctgtatatc ggtgaaaagc gcggcgggcc gctcctcgcg gccgggttgt tgctgatcat
    25441 catgtggata aactggtttt cggtggccct cgtgccgttg ttcggcctcg tcttgttctg
    25501 catgggggat acctatctca ccgtcagccg cgaccgcgga ttgttctcct gatcacgcat
    25561 gtgctgctca ccagagaaga ccacccgcac catggcagaa gttgttggtc tgggactgcg
    25621 catattcggt gtgcttctct gtgccgccct cgcactggcg acgaccggcg aggcagccgg
    25681 caaggtctcc ggtaccttga tcctgcacga tgcgctgacc ttgcccaatc attcggttcg
    25741 catagaagcg caggtcgctg tgacggccgc aggtccgccg ttgagcggtc tgatgctgca
    25801 cctgcagatc gacggaaaag acgttgcgac ggccaaaacc aatgaaggcg gccgggccgc
    25861 gttcgattat ctggcgaaaa tgcgaggcac taatgtcatc agtgtgtcga tcgatccgga
    25921 tgctcccgtt gcggcggaaa aagtcgacgc gaccctgtgt gtgtgggaac gccggcgccc
    25981 gattgtggtg gtcgaattag acgcgctcat ccagcagtct cccgccccgg caactcctgc
    26041 cgcatcgcct gggatagggg taacggatga caccgtgccg acgccggttc cggaggctgc
    26101 cgaggaactc tcccgactga cccagtacta ctacaacgtg gtctacgtct cgaacaacga
    26161 gcaggggccg atgggaaatg tctccagtgg acaggtgcgc cgctggttgg aagcgcatca
    26221 gtttccggtg gggttcgtgg caacgccgac atcaggggga ctgaacgcga ccctcgatgc
    26281 gttgaaacat gacggatgga cgacgatgaa atccggcatc gggcgcacga ggggatttgc
    26341 ggatatgctg ctgcagcacc gcatggaagt cgtggtggtg ccggaattac ccaagggcga
    26401 attgcctcgc aaagccaagg ctgcgaagga ttggaaagag gtgcgaaaga agttgtaggt
    26461 ggcgtctgga tagccatatg gtcgcagaac gcgcacctca gaagatgcat cgttcgatgc
    26521 gcgcaatcta tggccatccg gacatcacct tgcgtgaggc aaaggtaaag actgagagag
    26581 cgacggaacg tgcagagaag cgcgcaacgc ggcgatggcg gatggtggga aggtcattca
    26641 ctccatccgc gaatcatggt atgttcagcc cccaatcagg tgtcatctca ttgaaggagc
    26701 acaagccggt atgttgctag aaggtaaaaa gggactgatc atcggtgtgg ccaataagca
    26761 cagtatcgcc tgggccatcg cgcaggctgc cgcgcgggaa ggcgcgcaga tgttgtttag
    26821 ctatcagaat gaacgattgc gcgaaaacgt cgaagaattg gtccagaccc tgccgggcgc
    26881 atcggcctgc gtgtgtgatg tcggagatga cagtcagatc gccgcgatga tgaaagaagc
    26941 cgctgagaaa ctcgggcggt tggattttgt tgtccattca ctcgcttttg cgccccgcga
    27001 agaactcacg ggggaatttg tgaataccac ccgccaggga tttgcgacgg ccctcgacgt
    27061 gagcgcctat tccctcgtcg ccgtcacgcg ggcggcgatg ccgttgatga cggacggcgg
    27121 ttcagtcgtc acgctgacct atctcggcag cgagcgggtc gtgccgcact ataacgtgat
    27181 gggcgtcgcc aaagccgcgc ttgaagcaac ggtgcggtac cttgcccacg atctaggacc
    27241 caagaacatc cgcgtgaatg ccgtctccgc cggaccgatc aaaacgctgg cggctcgcgg
    27301 agtatcggga atcagcaaaa tggtcgacca ccatcgcgcg ttcgcaccgc ttcggcgggc
    27361 gaccgagcaa ggcgaagtcg gcgacacggc gttgttcctc gtcagtcctt taggccgcgg
    27421 catcaccgga gaggtgatct acgtcgatgg gggataccat attctcggct c
//
LOCUS       DAOQEW010000028        27228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_405_length_27228_cov_8.581312, whole genome shotgun sequence.
ACCESSION   DAOQEW010000028 DAOQEW010000000
VERSION     DAOQEW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 27228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27228
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_27228_cov_8.581312"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLS35_05060"
     CDS             complement(<1..361)
                     /locus_tag="PLS35_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNI18056.1"
                     /translation="MQRIREGLKTRFRSLVTQRTGLDEMAVDDLATSTKLQSWEAVQI
                     PERLRFSSRLAILLVGFFMLIVAFVPWTQTITVTGQLSAYTPFERPQDIEAQITGRIR
                     KWHIFEGVRVKAGDVILE"
     gene            535..1089
                     /gene="purE"
                     /locus_tag="PLS35_05065"
     CDS             535..1089
                     /gene="purE"
                     /locus_tag="PLS35_05065"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNI18057.1"
                     /translation="MPKKKRADRALVGVLGGSTSDFPILEKAVAMLNELGIPNELLVV
                     SAHRTPDRLFAYAEQAVARGIQVIIAGAGGAAHLPGMVAAKTLLPVIGVPIPTEHLRG
                     FDSLLSIVQMPRGIPVATVAIGGAENAAILAAQILGVQSASVRQRLERFRAAQTQKVL
                     DSQDDARLSPPFPMSRGTRTSRRP"
     gene            1086..2252
                     /locus_tag="PLS35_05070"
     CDS             1086..2252
                     /locus_tag="PLS35_05070"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNI18058.1"
                     /translation="MNVTVIDPGATLGVLGGGQLGAMFATAARRMGYAVNVWDPDPEA
                     PAHRIADHSLIRPFTDADARKEFTQRVRAVTYEWENVPADLCEQLECELPVRPSSRVL
                     RVIQDRIEQKTFLRSRGLPVPVFATLASPEELGRQTMPAYPLVCKTATAGYDGKGQWK
                     ILRAEDCPAVQQELARTRRPGSRWILEEWLPFQRELSILVVRGADGASQTYPLVDNVH
                     EEGILRQTIVPADVPQPVADLAGTIACDAVQALDGVGVFCVELFLMADGRLVINEIAP
                     RPHNSGHYTLDACTVSQFEQQVRTLCGLPLGEIRLLSPAVMLNLIGEDVRLVTHSPAA
                     QDLLRVPGAIVHLYGKRTIRPKRKMGHISFLAPTRAEALTAASTFRSRFAPIHS"
     gene            2507..4366
                     /locus_tag="PLS35_05075"
     CDS             2507..4366
                     /locus_tag="PLS35_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18059.1"
                     /translation="MSVPLSSDLDQTGRHPTMRGLGRLFSHSRVRVMFLQSLVAGILS
                     YELLVSNETIYGQNVSRMVALGLMLISMGIILLPKAALESTWFPGALISINTLLVTGT
                     IYLSGNASSELYLTYFLLLLIASSAPSLKQLLGLSVIICAGYGILVYEHAMQSGTLAV
                     GHLLGIPVLLIMSVFYGLTLETVAVERRRNRLLREDVQGLKQSEQVLEERRTQLETRV
                     QGLKQGLSRANQEIRQGMVERTGLERQLREAQKFEAMGRLASRLAHEFNQVLAVIGAQ
                     TGAIVSKLKPDDPLHGPVEDIFRSGERAATLTAQLLALGVHETSIRDTLSLGEAIVSM
                     REALQALLPGRIDVRVPDESKPVLVEIGHDRLECLLLHLVANARDAMPSSGRVDISLE
                     VVTGELLPAEQLEKNPRKRMARIAVSDSGGGMNLDVQAQMFEPFFSTKETHAGLGLTL
                     VYGIVRQCGGAVEVQSQPGQGTTVRVYLPLVERNAVLRDSRVLPEALSKGAETVLLVE
                     EDEIARKLALSTLHGHRYHVLEAGSAVEALLVAQQYQGPIHLTVSHLSMPEISGRELA
                     KRLVLQHPKMKALFVSGFSDETIASHRVNRKYFLQQPYRQHDLAGKVRELLDV"
     gene            complement(4383..5087)
                     /locus_tag="PLS35_05080"
     CDS             complement(4383..5087)
                     /locus_tag="PLS35_05080"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNI18060.1"
                     /translation="MLPLSDDNPTERTPVVTVAFIVACSLVFLYQSSLGSTSGEAFVY
                     QYGAIPSLVFGQAVLPPELTRIPAYATVLTSMFLHGSWMHLIGNMLYLWVFGNNIEDI
                     MGHVRFIVFYVGCGILAALSHALTDPTSTVPMVGASGAISGVLGAYLLLFPHARVLLI
                     APVVGATYVPAGIVLGLWFVMQLLNGGASLGSQGGGVAFFAHIGGFIAGMVLIGFFKR
                     PEVRFFKPGRTESFRY"
     gene            complement(5110..5967)
                     /locus_tag="PLS35_05085"
     CDS             complement(5110..5967)
                     /locus_tag="PLS35_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNI18061.1"
                     /translation="MSAATMRTLSSSPPTLILYHAECADGFGAAWAIWRRYPDAEYRP
                     VKHGEAPPTNLAGHHIVMVDFSYNRSTLEAMAKDAASLVVLDHHITAEQALADLPYAY
                     FDLNKSGAVLGWEWAHDEPAPWLLRYIQDKDLWHWALPNSREISAALASHPFDFELWT
                     RFEQRELEREGRAILRYENELVTKLASHATLVEFEGATVPSVQSSVLTSQIGERLSAE
                     HPFCVIWHDRNGRRYFSMRSREEGTDVGAIAASFGGGGHTHAAGFSVPLQADGLPQAN
                     PRLPRPAAS"
     gene            complement(6062..7117)
                     /locus_tag="PLS35_05090"
     CDS             complement(6062..7117)
                     /locus_tag="PLS35_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNI18062.1"
                     /translation="MKTPAARSKPVPTKDEILTQITTLLDRPDDDVQAALMKEILAGV
                     IRLSESQLDVLDLKIVNRALKELRHAFRVFQGYRDRLKISVFGSARTPADDPNYQLAF
                     QFARRMVEEGYMTITGGADGIMRASQEGAGREHSFGVNIMLPFEQGANAVIADDPKLV
                     TFKYFFTRKLMFQKESHAIALFPGGFGTHDEGFEIMTLVQTGKSDPKPIVCLQAPGCD
                     YWNHWNSFITDQLLKRRLINAEDLSLFTIVDNVEDAVTEIRTFYRRYHSLRFVGRQLA
                     IRLKTPLTAEQVQEVQHQFKDMLTEGTFEQRPSLPEEIDEPGLKDLARLTFSFNRRNA
                     GRLRQLINHLNHLPATT"
     gene            complement(7219..7929)
                     /locus_tag="PLS35_05095"
     CDS             complement(7219..7929)
                     /locus_tag="PLS35_05095"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI18063.1"
                     /translation="MMTPGVVQIPGDVSVSEAASLLDREQMPCLLVKDGEAAFGIMTS
                     GDIVKKVVAQGLEPHDVEVRSIMSKPVHSVECDQILDDATSVMASTGTSLLIVTKQGQ
                     PVGILTARDLALAPKRCETCLPATIRVTNGEGEGAKHTATIRQLSHVGASIESRTLLL
                     PGTSIVLSFILPGTDLSFSLQGTILNSNYEPEFHEDRNVLGTYSGIDIQFLSLSSSDE
                     SKIRAWVLQNLPRASDLS"
     gene            8190..9674
                     /locus_tag="PLS35_05100"
     CDS             8190..9674
                     /locus_tag="PLS35_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNI18064.1"
                     /translation="MKSEFTERAHRLPAHGLGLSVDVYSPDLLELVRALRDAALEPDY
                     LEIFKATTSAQQWMRQQLPDLKLTYHGEGLWSTQPEFCRSGSGRQGVAEACAQLAALR
                     SAWLNHECATKQMAGYAFGTYLPPLYTELSARMTAENVAYLQSQVDQDAHRREIDPAL
                     VLLEMPPLTYFGCGALPIPDFFRAVTDQASCGLVLDIGHLWTVYRYTGSWQRQRLEEF
                     AAQFLDAFPMERVIEIHVAGLAELTIPHASGIDASGNALPYWIDAHGAPIPGVLFDLL
                     AQVLSHPGLTSLKGVALEVDTKPIAMIVEEFKQFRERFGTTVQAMLHRDSPAPVQPQR
                     SPNARSAERRQLTSEEEATLQQQYRHYVQLVTTQGDLSSISIRNLSLLGGSLDDLNRY
                     RETYLPHELLHWGGELQDMFPDTCRALTESDVPLDRFVAVWFSKPRPEQDDYDFFLLK
                     IDRFVEFATHACPMAAMIVMREAEELRAAYRQANEPIGSSQVRA"
     gene            9671..10855
                     /locus_tag="PLS35_05105"
     CDS             9671..10855
                     /locus_tag="PLS35_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNI18065.1"
                     /translation="MNFWTTLPQPILGLAPMDGVTDAAFRRVVASQGRPDITFTEFTN
                     VNEICRGPDHLLSSLIYSEAERPIVAQLYGKDPEQFYRAAQVVCELGFDGLDINMGCP
                     SKSVAASGSGAALIKTPDLAHAILRAARQGLEDWAGGQSIHQQGFKPSRIEFIHELNH
                     RRSGSPVVPRRLLPLSIKTRLGFDCVVVERWVEHLLEVRPAAITVHGRTLQQMYRGAA
                     DWTAIAEAARVARDTETLILGNGDLNTLVDAVRRAADSRVQGVLVGRGTLGAPWFFRE
                     KEQARRAFVEQVDLSTLPATMWEPPVSLRHRMQVMLDHARQYEAIAGLECFRSIRKHL
                     GWYCKAFPHAAAMRGKMFGVSNVQDVERIVAEFCQDLILEEAAGSDAAPIAPQFPYPC
                     AS"
     gene            10860..11492
                     /locus_tag="PLS35_05110"
     CDS             10860..11492
                     /locus_tag="PLS35_05110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNI18066.1"
                     /translation="MRLILASTSPRRHELLTLLGVPFDVVAPPFIEQVQPHLSADSQA
                     VEFAAGKARSCVSSCPDALILGSDTLINLGSEVLGKPLDLAGAEVMLRRLAGQTHRIV
                     TAVALAGPEPERCDVRLAEVLVTMKPLNEATLANYLRTGDSLGKAGAYSIQGGGAAFI
                     ERIEGDFTAAVGLPLQLVAEMLRQRGLSCPVDIDQLYASRPYPNWDRFRP"
     gene            11599..12273
                     /locus_tag="PLS35_05115"
     CDS             11599..12273
                     /locus_tag="PLS35_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18067.1"
                     /translation="MRVNDGSVGRRGQTHAVVGLVIGAMALAISGPAWAIDVKLSPED
                     AKKALEAGRAPMEKAASPEDVKKILQQASLVTRVGADPEKDPCGASAILRTKRYRLEA
                     FGRQEAAESKKQKKDVRMPDEFIQKVVDMPNMEVEVQLCGDDEYFAEKADIVFQQKGK
                     NIRPVDLSPADRGRKNDGTGPAYRSRFTARFGYESFDPHAKTTIIVTLQDGQTMQFDA
                     DFSQVK"
     gene            12423..13391
                     /gene="pdhA"
                     /locus_tag="PLS35_05120"
     CDS             12423..13391
                     /gene="pdhA"
                     /locus_tag="PLS35_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNI18068.1"
                     /translation="MDRADLFSLYRQMLLIRRFEEKSAEMYALAKIAGFLHLYIGEEA
                     IAVGAIAALRPDDYVISAYRDHGHCLARGSDPGKVMAELFGKATGLCQGKGGSMHLVD
                     LPKRFMGGYAIVGGHIPLAAGLAFASKYQKQDLVTVCFFGEGAVPSGQAHEAFNLAAL
                     WKLPVIFICENNRYGMGTPVQRAVALYENVAEAARSYGITAERVDGMDVLAVRGLMCK
                     VVDQVRAGQGPYFIEAMTYRFMGHSMADPSHGHYRSKEEVEKYRKRDPLVVLKETMVS
                     QAQCSEADFKQLERDIADVVAAAVKFADDSPFPDPASLHADILVED"
     gene            13395..14372
                     /locus_tag="PLS35_05125"
     CDS             13395..14372
                     /locus_tag="PLS35_05125"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNI18069.1"
                     /translation="MLLSYREALNQAMREEMRRDSRIFLIGEEVGYYQGAFKVTKGFV
                     EEFGPQRVVDTPITEAGFTGLAIGAAMAGLHPIVELMTMNFGIVALDQIVNNAAKIRY
                     MSGGQLSVPLVIRGPGSAAHQLGAQHSQSLEAWFCHVPGLKVIAPATPHDAKGLLKSA
                     IRDHNPVIFIEAQLLYGTKGEVGDGEYTIPLGQAEVKREGTDVTIVAYSKMLLVALDA
                     ADQLRREGLDVEVIDPRTLKPLDLETIVTSVTKTGRLVIVEEGWRFCGLGAQIAESIY
                     SAAFDYLDAPIVRVTGEDVPIPYSRPLEDAAIPDVPRVIAAVKSLCGAA"
     gene            14436..15635
                     /locus_tag="PLS35_05130"
     CDS             14436..15635
                     /locus_tag="PLS35_05130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNI18070.1"
                     /translation="MASRVVMPKLTDTMEEGVLLAWKKHEGDPVHAGEVIAEIETDKA
                     VMDLEAFAPGVLRKVLVHDGETVQSGKLIAVIAEADEDIASALSDGVTAAASTGTGPK
                     SAPSTDAAASVSSGRPRGERVLASPRAKALAAERGVELSTLTGTGPGGRIVEDDVTAA
                     SASVAHALPAGTDQPLSQMRKAIARSTVQSKAPVPHFYLTVDIDMEQAERVRDEFKQS
                     RQPHPSITDVLIKAAALALKRHPEINVSFTGEAIRRYTQIDIGVAVGMDDGLITPVVR
                     NCGGKTLAEISVETKQLIERARHKRLSPQEYTGATFAISNLGMFDVDQFIALLMPPQA
                     ASIAVGAIRDVPVVSRGAVVAGRRMKVTLSCDHRALDGLMGAQFLKEFKRVLEHPEEL
                     LTPVVTP"
     gene            15632..16540
                     /gene="lipA"
                     /locus_tag="PLS35_05135"
     CDS             15632..16540
                     /gene="lipA"
                     /locus_tag="PLS35_05135"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNI18071.1"
                     /translation="MSFIQIDSRHTAEPQEPSRSQSRRLPPWFKVRLQTGPDYHDIRN
                     TMDRLKLHTICEEARCPNVWECWNARTATFLILGDICTRRCHYCSVATGRPHAVDHEE
                     PLRVAEAIHALNLRHAVITSVNRDELEDGGASVFAETIRHIRRLIPSCTIEVLIPDFE
                     GNEAALAAVAAERPDILNHNIETVRRLFPSIRPQGKYQRSIELLGRAKQMGMTTKSGL
                     IVGMGESTDEAREVMRDLRSVECDIMTIGQYLQPTKEHLAVARFYHPDEFAALKEEGL
                     ALGFRHVESGPLVRSSYHAEQQVSGR"
     gene            complement(16623..17855)
                     /locus_tag="PLS35_05140"
     CDS             complement(16623..17855)
                     /locus_tag="PLS35_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI18072.1"
                     /translation="MSFLWLTIQSALRVLRRNPLRAGLTMLGIIIGVGAVVGMVSLGQ
                     GATASVQREIASLGTNVLIIIPGATTTGGVRGGLGSVSTLTVDDARDIEKRVGDIGLV
                     TYASRSVLQAIHEHKNWNTIAIGTTTAFTDLRNWPVANGSFFTQADEDAAAKVVVLGK
                     TVADNLFERGEEIIGAQIRIKNVPLRVIGVLSSKGQSLSGQDQDDIVILPFTTAERKV
                     LGTKFLGTVGIIMVATQHRYSVPTAVEEITELLRVRHRIHPAEENDFTIRTMEDVAKT
                     VAGASRTMMMMLLSIASISLVVGGIGIMNILLVSVTERTKEIGIRMAVGAKRAHILLQ
                     FLVEAIILTAIGGVAGVVFGILGARLLTHLIGWPTIISSQSIAVAFLFSLAVGIFFGL
                     YPANKASRMNPIEALHYE"
     gene            complement(17866..19185)
                     /locus_tag="PLS35_05145"
     CDS             complement(17866..19185)
                     /locus_tag="PLS35_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18073.1"
                     /translation="MTSDHSDIDPPRAAHPTPVIVNAVPVVQGQGHGRAPASISQQTL
                     PRHRRRAPIVIGMVGVLTLLAAALWHWWSNDPVNGHYKTVTIDRGPITALVTATGAVN
                     PVISVQVGSQVSGKIAKLYADFNSVVREGQILASIDQKPYQAKVSQAKAALKSAQAGL
                     AKARNMLVQKTLELNRMAALRKEQFVAQADLDLARTNARDAEAQVDVSDAQVDQAKAT
                     LASAELDLGYTTIFSPVNGTVVSRNVEEGQTVVASFQTPTLFVLAQDLTHMQVIANVS
                     ESDIGGVTEGKSADFRVDAYPRESFHGIVTQVRNAPVSIQNVVTYDVIISVDNPDLKL
                     KPGMTANITIVTARNEDALRAPNAALRFRMPGVAVDRKTPQLWVLDASGHVRSVPVSL
                     GIADSLYTDITSSDVHEGESAIVGLATAEDTAQEKLPPGFEFGPKMR"
     gene            19440..20819
                     /locus_tag="PLS35_05150"
     CDS             19440..20819
                     /locus_tag="PLS35_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18074.1"
                     /translation="MTEEWGAVLVVDDDADMRSLLCDVLQGRGHQCTGVGGGDEALKE
                     LSEEDYAVVLTDLRMKGMQGTELLAEIKQRYPDIGVILMTAFGTVETAVDAMRHGASD
                     YLTKPVKTEELVRVVERAVREASLRREVSRLRKEVHKEYSFHQILGKSKPMQAVFDLI
                     RRVADSPTNVLITGESGTGKELVAKAIHYNSDRRDAPFVPVNCAAIPEQLLESELFGH
                     MRGSFTDAKMDKRGLFEEAQKGTLFLDEISELPLMLQAKLLRAIQEREIRRVGATKSI
                     PVDVRIIAATNLNLVDEVKNKRFREDFYYRLNVIELRLPPLRERREDIPILVDAFLKK
                     CAAARGRQVKGLSEPALAMLADYAWPGNVRELENVIERAVTLSHGDMIGAEDLPIQVQ
                     GARGDRRILDEAAERTLPLHEVEKEYIVKILEKTGGNKYQAAHVLGIDRKTLYRKLAE
                     IEGKPHSDA"
     gene            20856..23537
                     /locus_tag="PLS35_05155"
     CDS             20856..23537
                     /locus_tag="PLS35_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18075.1"
                     /translation="MHPLSQKSLLLEYVGPALLTLGIFLLDLSVSAEYTVWLLYAIPL
                     ALTAANSRPGASLYGAGLVSLLVVIGGVASPTGVLPVSFWVNRFLGIGFMAAISFLFA
                     NRRNHSVPTVPPTMPEPAHKESTPTETVGAIAHAEAAVEGAVAGQRRAEAELHRDKLR
                     FEGIVQSAMDAIITVDESQKIVLFNQAAENMFQWSAEDMLGRPLDRLMPERFRSAHAE
                     HIREFGRSGITTRQMGALGVIMGIRSTGEEFPIEAAISQIGVAGTRYYTVILRDITVR
                     KRLEQELAEREALLRAIIETEPECVKVLALDGTVRTMNAAGLAMIEAASNAEIVGKDI
                     CHLVAVESRSVFRDIVRKAGEGEAGRLEFRIVGLQGTPRWLDTHVVPLRAPDGSITAV
                     LGVTRDVTEWKKAEQLLRQSEERYRRLLGVLPDAVLVVRDNRVFFANEQGLRLFGATR
                     AEDLQGKWIYDLIHRDYHEAVAERFRHLLAGGGAVPEAEERILRLDGAMVDVAVRAAQ
                     FRDEDGAGILVVLRDMTMRNVGQQRLKESEERLQSLLESMEDVIWSSSLDLTTLHYVS
                     PSVGQIYGRAAEEFVARPSLWMEVVHAEDRALAEQALRDLQAIGEYDVEYRILRRDGE
                     VRWVHDRGRIIKDETGHPVRIDGIASDITERKRLQAQLRRTERVAELGTVASGMAHEI
                     GTPMNVILGRAEYLMERSKEESVKKGLQTIISQVERITRVMNQLLAFARRRPVEHRAL
                     DLRQTIEDNLEIFQERLSHSHITVETSFADDCPCVHADADQMSQVLINLVMNAIHAMP
                     EGGTLRVMLAPMPDRGMVTLTVADTGHGMPQEVIAKIFDPFFTTKEFGKGTGLGLTVV
                     RGIIEEHSGTIQVESQPGAGTQFTIRLPIHVEPSRKS"
     gene            23795..24199
                     /locus_tag="PLS35_05160"
     CDS             23795..24199
                     /locus_tag="PLS35_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18076.1"
                     /translation="MNETTPAVLLVVEDDKDMRSLLCDELWGEGYQLREASNGVEGLE
                     AVMRAAPDLIVTDLKMPSGGFDYVHRLRDCVPACPIIVMTAFGDARTKEEAMKSGATA
                     YFDKPVRLSELKATVKRLLHRSDSLPEEPSFH"
     gene            24342..25745
                     /locus_tag="PLS35_05165"
     CDS             24342..25745
                     /locus_tag="PLS35_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18077.1"
                     /translation="MAGSHAPATPLSPFADPILAARLEALRQLADGLTDRVAVMDRDL
                     NVVYANDAAWGKSGKPPAGVKPAKCYESFVQKQDPCGTCPAESVFVTGEVLTVSCNTS
                     GDGTACGMHQAFPLISSAGEVGSILVLFHKRSDPPAIPAALPQGKLGASRPERTDSRL
                     GDLVGASPAMNQLFEMIRLVADSSATVLIQGESGTGKELVARTIHQTSYRRDKPFVVV
                     DCGALPETLLESELFGHVKGAFTGAAGAKPGLFEEADGGTIFLDEIADTSPTFQAKLL
                     RVLQEGEIKRVGGTQPIKIDVRVISATNKDLTDLVKAKAFRQDLYYRLAVLPVHLPPL
                     RERRADIPLLVTKFIADSCHRHRQELRQVSEEAMRALTAAPWPGNVRELQHYIERAVV
                     TTTHSELTCTDLVSLKFHLEPADLRTVGREAAKQAERARILQALQQTAGNRMKAARVL
                     KISRASLYNKLHDFGIQ"
     gene            26334..>27228
                     /locus_tag="PLS35_05170"
     CDS             26334..>27228
                     /locus_tag="PLS35_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18078.1"
                     /translation="MRSSVQSKRRRISRKAMLSLQAALLSGSLLAAPWVFAAGPGDQS
                     HEGHATPVAMPGWTQTLKGQTVVENAMEGRAGNAEKMEMQHHRLMEKLENQAQTDAKA
                     QQTSGAFNEMSMMHQYMGQDGSSFLLASDSSKGEPVMTSGGKCPANAPVKKYDVSMIN
                     IEISLNRWLDFYPGYMYVHTADIDKARAEEAKNKEAREKDGFDPGAVTTGNQGDVIQP
                     LVLRANQGDCVKMTLRNQMEGEDGSLVIQASSMIVSSTGKPATTTNPDTVVSPGKAQE
                     FEWYIHPNMQEGVRQFHSWSHD"
BASE COUNT         5521 a         7464 c         8574 g         5669 t
ORIGIN      
        1 gttcgagaat cacgtcgccc gctttgaccc gcacaccttc gaagatgtgc cacttcctga
       61 tgcgtccggt aatctgtgct tcgatgtctt gcggacgttc aaaaggcgtg taggcggaga
      121 gctggccggt taccgtaatc gtttgcgtcc acgggacgaa agccacgatc aacatgaaga
      181 agccgaccag gagaatggcc agccgtgaag agaaccgcaa tcgttcggga atttggacgg
      241 cttcccagga ctggagcttg gtggacgtgg ccagatcatc gacggccatt tcatccaagc
      301 ctgtacgctg ggtgacgagg gagcgaaatc gggttttgag tccctcacgg atacgctgca
      361 cgtaacccct ccacgcaccg tccactgtag agatgcgcac acgtatcata gtggaagaca
      421 aaggagttgg tcaattcaat gaggcgttcc gtcgtgcttc ggcttgaccc ttcgaccgga
      481 gaagggctat ggttcgccgc atcattcttt ccttcagcca gaggtgtcgt gtccatgccg
      541 aagaagaagc gtgcggatcg ggcgttggtc ggggtcttgg gcggcagcac atcggacttt
      601 cccatccttg aaaaggccgt ggccatgctg aatgagctcg ggattcccaa cgagctgctc
      661 gtcgtgtcgg cccatcgtac cccggatcgc ctcttcgcct atgcggaaca ggctgtggcg
      721 cgggggattc aggtcatcat tgccggagcc gggggagccg cgcatcttcc cggaatggtg
      781 gcggcgaaga ctctgttacc ggttatcggc gtgcctattc ccactgaaca tctccggggc
      841 ttcgattctc tactgtcgat tgtccagatg ccccggggaa ttccggtcgc aacagtggcg
      901 atcggcggcg cggaaaatgc ggccatcctg gcggcgcaaa ttctgggagt acagtccgct
      961 tctgtcaggc aacgcctcga acgattccgt gccgcgcaaa cacaaaaggt tctcgattcc
     1021 caggatgacg cccggttgtc ccctcccttc ccgatgtcac gcgggaccag gacgagccgc
     1081 aggccgtgaa tgtcacggtg attgatcccg gcgcgaccct gggtgtgctt ggtggaggcc
     1141 agctcggcgc catgttcgcc accgctgccc gccgcatggg gtacgcggtg aatgtctggg
     1201 accccgatcc tgaagcgcct gcccaccgga tagccgacca ttcactcatt cgcccgttca
     1261 ccgacgctga cgcccgtaaa gagttcactc aacgggttcg tgccgtgacc tatgaatggg
     1321 aaaacgtccc tgccgacctc tgtgagcaat tggagtgcga gctgccggtc cgtccatcaa
     1381 gccgggtgct ccgggtgatt caggatcgca ttgaacagaa gacgtttttg cggagtcgcg
     1441 gattgcccgt gccggtcttc gcgacactag cgtcgccgga ggaactcggc cggcagacca
     1501 tgcctgctta tccgctggtg tgtaagacgg ctacggccgg gtatgacggc aaggggcagt
     1561 ggaaaatcct tcgagccgaa gattgtccgg cggtgcaaca ggagttggcc cgtactcgga
     1621 ggcctggttc tcgatggatt ttggaagaat ggcttccgtt tcagcgtgag ctatcgattc
     1681 tggttgtgcg gggtgccgac ggcgcgtcgc aaacctaccc cttggtggac aatgttcatg
     1741 aagagggcat tctccgtcag acgatcgtgc cggccgatgt gccgcagccg gttgccgacc
     1801 tggccggcac catcgcgtgc gacgccgtgc aggcgcttga cggggtgggg gtattttgcg
     1861 tggaattgtt cctcatggcc gatggccggt tggtgatcaa cgaaatcgcc cccaggccgc
     1921 ataactccgg tcactacacc ttggatgcct gtacggtctc gcaatttgag cagcaggtgc
     1981 ggaccttgtg tggcctgccg ctcggagaga tccggttgct cagtcccgcc gtgatgctca
     2041 acctcatcgg tgaggatgtt cgactcgtga cgcactcccc tgcagcgcag gatcttctcc
     2101 gcgtgcccgg tgcgatcgtc cacctgtatg gaaaacgcac aattcgacca aagcggaaaa
     2161 tgggccacat ttcatttctc gctcctacgc gcgcggaggc cctcaccgcc gcgtcgacct
     2221 tccgctcgcg gtttgctccg attcactcgt aaaaaaacga cagcaggcgc gatcaggttt
     2281 aggctcggtg tgcgtctgga tggtatccgt ttctgtcggt tgagatcgtt cggccgctct
     2341 cagactgtcc gtttttgtga gacggggaga ggtcaccggc cgttcatggc cacccttcat
     2401 tctcctcctc ctgaaattgc gaagacttgc caggatggca agacggtatg ggacttgctg
     2461 tcatccccgc tggtttcaca cttctctccg aagggcaggc acagcggtga gcgttccact
     2521 ttcctccgat ctcgatcaaa ccgggcggca tcccaccatg cgaggactgg gccgattgtt
     2581 ctctcattcg cgagtccgcg tgatgtttct gcaaagcctc gtggccggca ttctgtccta
     2641 tgaattgctt gtgagcaacg agacgatcta tgggcagaac gtcagccgga tggtggcgct
     2701 cggattgatg ctgatcagta tgggaatcat cttgttgccg aaagcggcat tagagagcac
     2761 ctggtttccc ggcgcattga tcagcatcaa taccctgctc gtgacaggga ccatttacct
     2821 ctccggaaat gccagctcgg aattgtatct cacgtatttt ctactgctcc tcattgcctc
     2881 ctctgcgcct tcactgaaac aattactcgg cctctccgtc atcatctgtg ccggatacgg
     2941 catcttggtc tatgagcatg cgatgcagtc cggcaccctg gcggtggggc acctgctcgg
     3001 tattcctgtt ttgctcatca tgtcggtgtt ttacggcctc acgctggaaa cagtggcggt
     3061 cgagcgccgg cggaaccgtc tgttgcggga agatgttcag gggttgaaac agtcggagca
     3121 ggttctggag gaacgacgaa cgcaactgga aacccgcgtg caagggttga agcaagggct
     3181 gtcgcgcgcc aaccaggaaa ttcgccaagg catggtcgaa cgcaccgggc tggagcggca
     3241 gttgcgcgaa gcgcagaaat tcgaagcgat gggacgactg gcctccaggt tggcgcatga
     3301 attcaatcag gtcttggcgg tgatcggggc tcagaccggg gccatcgttt caaagttgaa
     3361 acccgatgac cccctgcatg ggccggtgga agacatcttc cggagcggag agcgtgcggc
     3421 aaccctcacc gcgcaactgc tcgcactggg cgtgcacgaa acgagcattc gcgacacctt
     3481 gtcgctcggc gaggcgattg tgtcgatgcg cgaggcgctc caggcactgc ttcccgggcg
     3541 gattgatgtg agggtacctg acgagtccaa gccggtgctg gtggaaattg gtcatgaccg
     3601 gttggagtgc ctgttgttgc acctggtggc gaatgcgcga gatgccatgc cgagcagcgg
     3661 gcgggtggat atttcgctgg aagtggtcac cggagagttg ttaccggcag agcaactcga
     3721 aaaaaatccc cggaagcgga tggcccgcat tgccgtcagt gatagcggcg gcgggatgaa
     3781 tctggacgtt caagcccaga tgttcgagcc gttcttctcg accaaagaga ctcatgccgg
     3841 tctggggctc acgttggtct atggaatcgt tcgtcaatgc ggtggagcag tggaggtgca
     3901 aagccagccc ggacaaggca cgacggtgcg tgtctatctg cccctggtcg agcggaacgc
     3961 cgtgctgagg gacagcaggg tcctccctga ggcgctgtcg aagggcgctg aaacggtgct
     4021 cctcgttgag gaagatgaaa ttgccaggaa gctggccctg tcgaccttgc acggccatcg
     4081 ctaccatgta ctcgaggcgg ggtcggcagt ggaggcactg cttgtcgcgc aacaatacca
     4141 aggaccgatt cacctcaccg tcagccacct ttcgatgcct gaaatcagtg gccgggagtt
     4201 ggccaaacgg ttggtcctgc aacatccaaa aatgaaggcg ttgttcgtgt ccggattttc
     4261 cgacgagacg attgccagtc atcgagtgaa tagaaaatac ttcctgcagc agccctaccg
     4321 gcagcacgat ctggcgggga aagttcgtga attgctggat gtctaagcga ggaatcgcga
     4381 cgctagtatc gaaatgactc ggtgcggccg ggtttgaaaa atcgtacctc cgggcgctta
     4441 aagaagccga tcagcaccat ccctgcgata aatccgccga tgtgcgcaaa gaaggccacg
     4501 cctcctccct gcgagcccag gctcgcccca ccgttcagca actgcatgac gaaccacaag
     4561 cccagcacga taccagccgg cacgtaggtg gcgcccacca ccggggcgat caacaagaca
     4621 cgggcatggg gaaagagcag cagataggcc cccagcacgc ctgaaatcgc tccgctcgca
     4681 ccgaccatgg gaaccgtcga tgtcgggtcg gtcaaggcat ggctcaacgc cgccaggatt
     4741 ccacatccca catagaagac gataaatctg acatgtccca tgatgtcttc gatgttgttc
     4801 ccgaagaccc agaggtacag catgttgccg atcaagtgca tccagctgcc atgcagaaac
     4861 atgctcgtca gaacggtcgc atatgcgggg atgcgtgtca gttccggtgg gagcacggcc
     4921 tggccgaaca cgagtgaggg gatggccccg tattggtaga cgaaggcttc accggaggtt
     4981 gacccgaggg agctttggta caggaagacg agtgagcagg cgacgatgaa tgcgacggtg
     5041 acgacaggcg tgcgttcggt cgggttatcg tcactcaggg gcagcatcgc aacacctgaa
     5101 acacaagggc tatgaggcgg ccggtcgcgg cagccgagga tttgcctggg ggaggccgtc
     5161 ggcctgcagc ggcaccgaaa atccagccgc atgggtatgg ccaccgccgc cgaaggatgc
     5221 ggcgatcgcg ccgacgtcgg tgccctcttc gcgggaacgc atgctgaagt atcggcgacc
     5281 gttacgatcg tgccagatca cgcaaaacgg atgctcggcg gacaaccgtt cgccgatttg
     5341 actggtgagg acggagctct ggaccgacgg cacggtggcg ccttcaaact ccaccagcgt
     5401 cgcgtgggag gccagtttcg tcacgagttc gttttcgtag cggagaatgg cccggccttc
     5461 ccgctccaac tcgcgctgtt cgaaacgcgt ccagagctcg aagtcgaacg ggtgtgacgc
     5521 cagtgctgcg ctgatttccc tgctgttcgg aagcgcccag tgccataaat ccttgtcttg
     5581 gatgtagcgg agcaaccagg gggccggttc atcgtgggcc cattcccagc ccagtactgc
     5641 gcccgacttg ttgagatcga aatacgcgta ggggaggtcg gccagtgcct gttccgcagt
     5701 gatgtgatgg tccagaacga ccaggctggc cgcatccttg gccatggctt ccaaggttga
     5761 gcggttgtag ctgaaatcca ccatgacaat atgatgaccg gcgaggttcg tcggcggcgc
     5821 ttcgccgtgt ttcacggggc gatactccgc gtcgggatac cggcgccaga tggcccaggc
     5881 ggcgccgaac ccgtcggcac attccgcgtg atacaggatc agtgtgggag gagaggaaga
     5941 cagggttcgc atggtggctg cgctcatagg ccaaccacct tagcatgtcc gttcatggag
     6001 actaaagagc tatctcagga ttaggcgagc cggcgtggct gccggcttcg cctgcattcg
     6061 ctcaggtggt cgccggcaga tgattgaggt gattgatcaa ttgcctgagg cggcctgcat
     6121 tgcggcgatt gaaggaaaat gtcagtcgtg caagatcttt caatcccggc tcgtcaattt
     6181 cttcggggag tgagggccgt tgctcgaacg tgccttccgt cagcatgtct ttgaattggt
     6241 gctgtacttc ttggacttgc tccgcggtca agggggtctt cagtctgatg gcgagctggc
     6301 gtcccacgaa tcggagggag tgatagcgcc gataaaacgt gcggatttcc gtgaccgcat
     6361 cttccacgtt gtcgacgatg gtgaagaggg aaaggtcttc ggcgttgatc agacggcgct
     6421 tgagcagctg atcggtaata aaagagttcc agtggttcca ataatcgcag cccggcgcct
     6481 ggaggcacac gatcggcttc ggatcgctct tgccggtttg cacgagggtc atgatctcaa
     6541 acccctcgtc atgggtgccg aacccgcctg gaaagagcgc aatcgcgtgt gactcttttt
     6601 gaaacatgag cttgcgcgtg aaaaaatact taaacgtgac gagtttggga tcatcggcga
     6661 tcacggcatt ggcgccctgt tcgaagggca gcatgatgtt caccccgaag ctgtgttcgc
     6721 gaccggcccc ttcctgcgag gcccgcataa tcccatcggc gcctccggtg atcgtcatgt
     6781 agccctcttc caccatgcgt cgggcgaact gaaaggccag ttgatagttc ggatcgtcgg
     6841 cgggcgtgcg ggctgagccg aagacgctga ttttcagtcg atcgcgatat ccttgaaaca
     6901 cgcggaaggc atgccggagt tctttgagcg cccggttgac gatcttgagg tccaggacat
     6961 ccagttgcga ttcagagagc cggatgacgc cggccaggat ctctttcatg agcgcggcct
     7021 ggacatcgtc gtctggccga tcgagcaggg tggtgatttg tgtaagaatt tcgtctttgg
     7081 tcggaacggg tttggaacga gcggcgggag ttttcatcaa tagtcaccta acgcacggta
     7141 aactccggac gggcatcgcc ggagaatgaa atcataacaa gccggggagc tcgaagcaaa
     7201 agattcatcg ggaggaactt aggagagatc cgaggcacgc ggcaggtttt gcaacaccca
     7261 ggcccgaatt ttcgattcgt cggacgacga gagggacaag aattggatat cgatgccgga
     7321 ataggttccc agcacgttcc ggtcttcgtg gaattccggc tcatagttgc tgttgagaat
     7381 ggtgccttgc agcgagaagc tcaagtccgt tccgggaaga atgaacgaca gcacgatact
     7441 cgttccggga agcagtaatg tgcgactttc gatcgacgcg ccgacatggc tgagctgccg
     7501 gatcgtggcg gtatgttttg ccccttcgcc ttcgccgttg gtcacgcgaa tggtggcggg
     7561 aaggcaggtc tcgcagcgtt tcggggccag ggccaggtcg cgtgcagtca ggatgccgac
     7621 cggttgtcct tgtttggtga cgatcaggag ggaggtgccg gtcgaggcca tgacgctggt
     7681 ggcatcgtca agaatctggt cacattcgac ggaatggacc ggcttcgaca tgatggaccg
     7741 cacctccaca tcgtgcggtt ccagaccctg ggcgacgact tttttgacga tgtcgccgga
     7801 ggtcatgatg ccgaatgcgg cctcaccgtc tttgaccagc aggcagggca tctgttcccg
     7861 atcgaggagc gaggcggcct cgctgacaga gacgtccccg ggaatctgta ccacgccggg
     7921 tgtcatcata tgggccacca taggggttgt cgtatgagtc ggtttgtcgc ggagtatgga
     7981 gccggttctt gtcatagtgt aagcctattt ctcgccgtga gaggggctaa cggtgcggcc
     8041 cggcgaccac cacgtcgaaa gaaagtatat cagatggatg tgccggaccc gtcttcccat
     8101 cttcccttgt caaccaagag gttaggcgct tagactgcac agaaactata gtcactatgg
     8161 ttgtttcaaa atatgctaaa tgcgatccga tgaaaagcga attcacagaa cgtgcgcatc
     8221 gccttcctgc acatggtctc gggctgtccg tggacgtgta ttccccggac ctgctggagc
     8281 tggtccgtgc acttcgtgat gcagcgctgg agccggacta tctggagatt ttcaaagcca
     8341 cgacgtctgc ccaacaatgg atgcggcaac aattgccgga tctcaagctg acctatcatg
     8401 gtgaggggtt gtggagcacg caaccggaat tctgccgcag cggttccggc cgacagggag
     8461 tggcggaagc ctgtgcgcag ctcgcggcgt tgcgcagcgc ctggctcaat cacgaatgcg
     8521 ccacgaagca gatggcgggt tatgcgttcg ggacctatct gccgccactc tatacggagt
     8581 tgtcggcgag gatgactgcg gagaatgtcg cctatctcca aagccaagtg gatcaggatg
     8641 cgcataggcg tgaaatcgat cctgcgctgg tactcttaga gatgccgccg ctcacgtact
     8701 tcggctgcgg cgcgcttcca attccggatt ttttccgtgc ggtcaccgat caggcatcct
     8761 gcggcttggt gttggatatc ggacatctat ggaccgtgta tcgctataca ggaagctggc
     8821 aacgacagcg gttggaagag ttcgccgccc agttccttga tgcgtttccc atggagcggg
     8881 tcatcgagat ccatgtcgca ggactggccg aactgacaat tccccacgct tctggaattg
     8941 acgcgagcgg gaatgcatta ccctattgga ttgatgcgca cggggctccg atcccaggcg
     9001 tcttgtttga tctattggcg caggtcctgt ctcatcccgg gctgacatcc ttgaaaggcg
     9061 tcgcactcga ggtcgatacg aagccgattg cgatgatcgt cgaggagttc aagcaatttc
     9121 gagaacgttt tggaacgacg gttcaagcaa tgctccatcg tgacagccct gcgccagtgc
     9181 aaccgcagcg atcgccgaat gctcgttcgg ctgagcgacg gcaactgact tcggaagaag
     9241 aagccacatt gcagcaacag tatcgccact acgtgcaact cgtcacgaca caaggtgatc
     9301 tctcctctat ctctataagg aacctctcgt tgctaggcgg gtcgctggac gatctcaacc
     9361 ggtatcgcga gacctacctg ccgcatgaat tgctccattg gggcggcgaa ttgcaggata
     9421 tgtttccgga cacctgtcgt gccctgacgg aaagtgatgt gccgttggat cggtttgttg
     9481 cggtctggtt cagcaaaccc agacccgagc aggacgacta tgattttttc ctgctgaaga
     9541 tcgaccgatt cgtggaattc gcgacacacg cgtgtccgat ggctgccatg atcgtgatgc
     9601 gtgaagccga agagctacgg gcagcctatc gccaggcgaa cgaaccgatc ggctcatccc
     9661 aggtgcgcgc atgaattttt ggacgacgct cccgcagccc attctcggtt tggctcccat
     9721 ggacggggtg acggatgccg cgttccgtcg cgtggtggcc tctcaagggc ggccagacat
     9781 cacgttcacc gagttcacca atgtgaatga aatctgccgc gggccggacc atctgctgtc
     9841 ctcgctaatt tacagcgaag ccgagcggcc cattgtggcg cagctctacg gtaaagatcc
     9901 ggagcagttc tatcgcgcgg cccaggtggt ctgtgagctg gggtttgacg ggctcgatat
     9961 caatatgggc tgcccttcga agagtgtggc ggcgtccggt tcaggggccg cgctgatcaa
    10021 gacgccggat ctggctcatg ccatcctgcg tgcggcgaga cagggattgg aggattgggc
    10081 cggtgggcag tcgatccacc agcagggatt taaaccctct cgcattgaat tcattcacga
    10141 gctgaatcac cggcgttccg gctctcccgt ggtgccccgc cggctgcttc cgctttccat
    10201 caagacgcgg ctggggttcg attgtgtggt ggtcgagcga tgggtggaac acttgttgga
    10261 agttcgcccg gccgccatta cggtgcacgg tcgaacgttg cagcagatgt accgcggtgc
    10321 cgccgactgg acggctattg ccgaggcggc cagggtggcc cgtgatacgg aaaccttgat
    10381 tctcgggaac ggcgatctca atacattggt tgatgccgtt cgacgtgccg ctgacagtag
    10441 ggtgcagggc gtgttggtgg ggcgtggaac cctcggcgcg ccgtggttct ttcgtgaaaa
    10501 ggagcaggca cgtcgggcgt tcgtcgaaca ggtcgatctg tcaacgcttc ccgccacgat
    10561 gtgggaaccg ccggtgtctc tgcgtcaccg catgcaggtc atgctggacc acgcgcggca
    10621 gtatgaggcg attgccgggt tggagtgctt ccgctccatc cggaaacatc tcgggtggta
    10681 ctgcaaagca tttcctcacg ccgcggccat gcgggggaaa atgttcggag tctcgaacgt
    10741 gcaggatgtc gaacgcatcg tcgcagaatt ttgccaggac ctcatactgg aagaagctgc
    10801 cggatctgat gccgctccca tagctcctca attcccatac ccctgtgcat cctgactcca
    10861 tgcgattgat tctggcttcc acctcgccgc gtcggcacga actattgact ctgttagggg
    10921 tgccgtttga tgtcgtcgct ccgcccttca tcgaacaggt ccagccccac ttgtcggcag
    10981 actcgcaggc ggtagaattt gctgccggta aagccaggtc ttgtgtgtct tcctgtcccg
    11041 atgccctcat tctcggaagc gacacgctca tcaatctggg ctctgaggtg ttgggcaagc
    11101 cgctcgattt agccggcgcg gaggtgatgc tccgccgtct ggccgggcag acgcaccgca
    11161 ttgtgacagc cgtggccctg gccgggcctg agcctgagcg atgcgacgtc cggctggcag
    11221 aagtgctcgt gacgatgaaa cctttgaatg aagccacatt ggccaactat ctgcggactg
    11281 gcgatagcct gggtaaggcc ggggcctatt ccattcaggg aggcggggct gcatttatcg
    11341 agcgcatcga gggcgatttc accgcggccg tgggattgcc gctgcagctt gtcgctgaga
    11401 tgttacggca gcgtggactg tcctgtccgg tcgacatcga tcaactgtac gccagcagac
    11461 cctatcccaa ctgggaccgc tttcgtcctt agccaggcca cgtcggtcac accccttctc
    11521 acgtcccgcc agattgaaat ggtcttccca atccttttac aatgcgccct tgttcagtga
    11581 cgtgtcaggg aggtactcat gcgcgtgaat gatggcagcg taggtcggcg tgggcaaacc
    11641 catgcggtag tcgggttggt gatcggagca atggcattgg caatcagcgg gccggcgtgg
    11701 gcgatcgacg tcaagttgtc ccccgaagac gccaagaagg cactcgaagc cgggcgagcg
    11761 ccgatggaaa aagcggcttc gccggaagat gtgaagaaaa tcttgcagca ggcttcgctg
    11821 gtcacgcggg ttggtgcgga tcccgaaaaa gacccttgtg gggccagcgc cattctgcgc
    11881 accaagcgct accgcttaga ggcgttcggc aggcaggaag cagccgaatc gaaaaaacag
    11941 aaaaaagatg tgcgcatgcc cgacgagttt atccagaaag tcgtggacat gccgaacatg
    12001 gaagtggaag tgcagttgtg cggcgatgat gaatactttg ccgagaaggc tgacatcgtc
    12061 tttcagcaaa agggtaaaaa tattcgcccg gtggatctca gcccagctga tcgaggcaga
    12121 aaaaacgatg gcacgggccc ggcctaccgg tcgcgtttca ccgcccggtt cgggtacgag
    12181 tcgtttgacc ctcatgctaa aaccaccatc attgtgacct tgcaggatgg tcagaccatg
    12241 cagttcgatg ccgacttttc gcaggtgaaa taatcctcag cttccggtgc gggtctgcag
    12301 acccgtaccg gtcacttgcg cgccccctcc cttctcagtg ttgttctttc attcgtgcga
    12361 tggtgctgac aaccgaacat cacggttgag tcgtcttgtc gccggcatgc ataatgacgg
    12421 acatggacag ggccgatctg ttttccctct atcgccagat gttgctcatc cgtcggtttg
    12481 aagaaaagtc ggcggagatg tatgcactcg ccaagattgc cggatttctg catctctaca
    12541 tcggagaaga ggcgattgct gtcggtgcca tcgctgcgct cagaccggac gattatgtca
    12601 tcagtgccta tcgcgaccac ggacactgtc tggcgcgtgg gtctgatccg ggcaaggtga
    12661 tggccgaact gttcggcaag gcaaccggat tgtgtcaggg caaggggggg tcgatgcatt
    12721 tggtggatct cccgaagcgt tttatgggcg gttacgccat cgtgggcggc catattccgc
    12781 tggcggcggg gttggccttt gcctcgaagt accagaagca ggacctggtg acagtctgtt
    12841 tcttcgggga aggtgcggtg ccgagcggcc aggcgcatga agcgtttaat ctcgcggccc
    12901 tatggaaatt gccggtgatt ttcatttgtg agaacaatcg atatggcatg ggcacgccgg
    12961 tgcaacgggc cgtggccttg tatgagaatg tggcggaagc cgcgcgttcc tatgggatta
    13021 cggctgagcg cgtcgatggc atggatgtgt tggccgtgcg tggtttgatg tgcaaggtgg
    13081 tggatcaggt tcgagcaggg cagggaccgt atttcattga agccatgacg tatcggttca
    13141 tggggcactc gatggccgat ccgtcccatg gccactaccg gagcaaagag gaagtggaaa
    13201 aatatcgcaa gcgcgatccg ctggtcgtct tgaaagagac catggtgagt caggcgcaat
    13261 gtagcgaggc cgacttcaag caactcgagc gggatatcgc tgacgtggtg gcggctgcgg
    13321 tgaaatttgc ggacgacagc ccgttcccgg atcctgcgag cctgcatgcc gatatattgg
    13381 tggaagacta atccatgctg ctgtcttacc gggaagccct caatcaggcc atgcgggaag
    13441 agatgcggcg ggactcgcgt atctttttga tcggcgaaga agtcgggtat taccaaggcg
    13501 ccttcaaggt caccaaggga ttcgtggagg aattcgggcc gcagcgggtg gtggatacac
    13561 ccattaccga ggccgggttt accgggctgg caatcggcgc ggccatggcg ggtctccatc
    13621 cgatcgtcga gctgatgacg atgaatttcg gcattgtcgc gctagatcag atcgtgaata
    13681 acgcggccaa gattcggtat atgtccggcg gtcagctctc ggtgccgctg gtgattcgcg
    13741 ggccgggcag cgcggcgcat cagttgggag cgcagcactc acagagttta gaggcgtggt
    13801 tctgtcacgt gccgggcttg aaggtgatcg ctccggccac gccgcacgat gcgaagggtt
    13861 tgctgaagag cgcgattcgc gatcacaacc cggtgatctt cattgaggct caactgctct
    13921 atggcacaaa gggtgaggtc ggcgatggtg agtacaccat ccccctcggc caggctgagg
    13981 tgaagcgaga aggcaccgat gtgaccatcg tggcctattc aaaaatgttg ctggtggcgt
    14041 tggacgcagc cgaccagtta cgccgtgaag gtctcgatgt ggaggtcatc gatccccgta
    14101 cgctcaagcc cctcgacctt gagaccatcg tcacctcggt caccaagaca gggcggctgg
    14161 tgatcgtgga ggaagggtgg cggttctgtg ggctcggggc gcaaattgct gagagcatct
    14221 acagcgcggc gttcgattat ttggacgcgc cgattgttcg ggtcaccgga gaggacgtgc
    14281 cgatccctta tagccggccg ttggaggatg ccgccattcc ggacgtgccg cgagtcatcg
    14341 cagccgtcaa atcactatgt ggtgccgcat gaggcatggt tgccgaagga gacaggccgg
    14401 tcgtccaaga ccgtccgctt cgtggaggag aatacatggc tagccgcgtc gtcatgccga
    14461 agctgaccga caccatggag gaaggcgttc tgctcgcctg gaaaaaacat gagggtgatc
    14521 cggtgcacgc gggcgaagtg atcgccgaga ttgaaaccga caaggccgtc atggatctgg
    14581 aagccttcgc tcccggcgtc ttgcggaaag tcctggtgca tgatggcgaa accgtgcaat
    14641 cgggcaagct gatcgccgtc atcgcggaag ctgatgaaga cattgcttcg gccttgtcgg
    14701 atggcgtgac ggctgcagca tcgaccggta ctggaccaaa gtccgcaccc tccacagacg
    14761 ccgccgcatc tgtgtcgtca ggtcgccctc gaggagaacg ggtgctggcc tctccgcgtg
    14821 cgaaagctct ggcggctgaa cggggtgttg agctctcgac cctcaccggc accggtccgg
    14881 gtggccgcat tgtcgaagac gatgtgaccg ccgcatccgc ctcggtcgcc catgccttgc
    14941 ccgccgggac agatcagccg ctgagtcaga tgcgcaaggc cattgccaga tcgacggtgc
    15001 aaagcaaggc tccggttccg catttttatc tcacggtcga catcgatatg gaacaggccg
    15061 aacgggtgcg cgacgagttt aagcagagcc gtcagcccca tccgtcgatc accgacgtgc
    15121 tcatcaaggc tgcggcattg gcgctcaagc ggcatccgga gatcaacgtc tcgtttacgg
    15181 gtgaggccat ccggcggtat acccagatcg atatcggcgt ggcggtcggt atggacgatg
    15241 gcttgatcac gccggtcgtg agaaattgcg gggggaagac gttggccgaa atttctgtag
    15301 agacgaagca gttgatcgag cgagccaggc acaagcgatt gtcgccgcag gaatacacgg
    15361 gggcgacctt tgccatctcc aatctgggca tgttcgacgt ggatcagttt atcgcgctgc
    15421 tcatgccgcc gcaggcggct tccatcgcgg tgggcgcgat ccgcgatgtg ccggtcgtgt
    15481 ccaggggagc ggtggtcgcc ggacggcgga tgaaggtcac gctctcgtgt gatcaccgtg
    15541 cgttggacgg actgatgggc gcgcagttcc tcaaagagtt taagcgagtg ctggagcatc
    15601 cggaggaatt gctcacgccg gtggtgacgc catgagcttc attcaaatcg attcacgcca
    15661 caccgctgag ccgcaggagc cgtcacggtc gcaatcccgc cgcctgcccc cttggttcaa
    15721 agtccggctg caaaccggtc cggactatca tgatattcgc aacaccatgg atcggctcaa
    15781 gctgcacacc atctgcgaag aggcgcgctg cccgaatgtg tgggaatgtt ggaacgcgcg
    15841 gaccgcgaca tttctcattc tcggcgacat ctgcacgcga cgctgccact actgctctgt
    15901 ggccaccggg cggccgcatg ccgtcgatca cgaggaaccg ctgcgcgtgg cagaagcgat
    15961 tcacgcgctc aatttgcgcc atgccgtcat cacctcggtc aatcgcgacg aacttgagga
    16021 tggcggggca tcggtctttg cggagacgat ccgccatatc cggcggttga tacccagttg
    16081 cacgattgag gtcctcattc ccgacttcga aggcaatgag gcggcgctgg cggcggtggc
    16141 ggctgaacgg ccggatatcc tgaaccacaa tattgaaacc gtgagacggc tgttcccatc
    16201 gattcggccc caaggcaaat atcagcgatc aatcgagttg ctgggacgag ccaaacagat
    16261 ggggatgacg accaagtcgg gattgatcgt cggcatgggc gaatcgacgg acgaagcccg
    16321 cgaggtcatg cgtgacctcc gttcagtgga gtgcgacatc atgaccattg gccaatatct
    16381 ccaaccgacc aaagagcatc tcgccgtcgc gcgcttttac catcccgacg aattcgctgc
    16441 gttgaaggaa gaaggccttg ccctcggctt ccgccacgtt gaatctggcc cgctggttcg
    16501 tagttcctac cacgccgaac aacaagtgtc aggacgttaa aaaatctccg gctcttcctt
    16561 tgtcccagac ggcccagcct acaaccgtgt cgaacagtct gccatctcgt tgtgagctgc
    16621 ccctattcat agtgcagcgc ttcgatcgga ttcatcctcg aggccttgtt ggccggatag
    16681 aggccgaaaa agatgccgac ggccagtgag aacaggaatg cgaccgctat ggattgggaa
    16741 gaaatgatgg tgggccagcc gatcaggtgt gtcaggagac gcgctccgag gatgccgaag
    16801 acaacaccgg ctacaccacc gatcgcagtg aggatgatcg cttcgaccag aaattgcagc
    16861 agaatgtgcg cgcgtttggc tccgaccgcc atgcgaatgc cgatctcctt cgtgcgctcc
    16921 gtcaccgata cgagcaggat attcatgatc ccgatgccgc cgaccaccag cgagatggag
    16981 gcgatgctca ggagcatcat catcatggtg cggctcgctc cggccaccgt tttagccacg
    17041 tcttccatcg tgcggatggt gaagtcgttt tcctccgcgg gatgaatccg atgtctcacg
    17101 cgcagtagtt cggtaatttc ctccaccgct gtcggaacgc tgtagcgatg ttgggtggcg
    17161 accatgatga tgccgaccgt tcccaggaac ttggtgccca gaacctttcg ttccgcggtg
    17221 gtgaacggga ggatgacgat gtcgtcctgg tcctgccctg agagggattg cccctttgac
    17281 gagaggacgc caatcacgcg caacgggacg ttcttgatcc ggatctgcgc gccgatgatc
    17341 tcttcaccac gctcgaacaa gttatccgcg actgttttcc ccaagaccac caccttggcg
    17401 gcggcgtctt catcggcctg cgtgaaaaaa ctgccattgg ccaccggcca attgcgaagg
    17461 tcggtaaagg cggtcgtcgt gccgatggca atcgtgttcc agttcttgtg ctcgtgaatg
    17521 gcctgcaaca ccgaacgtga ggcataggtg acgaggccga tgtcgccgac gcgtttttca
    17581 atgtcccggg cgtcgtcgac tgtcagtgtc gagacgctgc cgagtccgcc gcgcactccg
    17641 cctgtcgtgg tagcgccggg gatgatgatc agcacgttcg tgccgaggct ggcgatttcg
    17701 cgttgcaccg aggcggtggc gccctggccg agactcacca tgccgacgac ggcgccgact
    17761 ccgatgatga tgccgagcat ggtcaggcct gcgcggaggg gattgcgccg gagcacgcgg
    17821 agggcggact gaatcgtcaa ccaaagaaaa ctcatgagga atgtgtcacc gcatcttcgg
    17881 cccgaactca aatcccggcg gcaatttctc ctgagcggta tcttcggcgg ttgcgaggcc
    17941 cactatggcg ctctcccctt cgtggacatc gctcgacgtg atgtctgtat acaatgaatc
    18001 cgcaatgccg aggctgaccg ggaccgatcg cacgtgcccc gacgcgtcca gtacccagag
    18061 ctgaggagtt ttacgatcca cggctacacc gggcatgcgg aatcgcagtg ccgcgttcgg
    18121 tgcgcggagc gcatcttcat tgcgtgcggt cacaatggtg atgttggcgg tcatgcctgg
    18181 tttaagtttg aggtcagggt tgtcgacaga aatgatcaca tcgtaggtca cgacattctg
    18241 aatgctgacg ggcgcgtttc ggacctgtgt gacgatgccg tggaatgatt ctctgggata
    18301 ggcatcgacc cggaagtccg cggatttgcc ttcagtgacg ccgccgatat ccgattcgct
    18361 gacgttggca ataacctgca tgtgagtgag gtcctgcgcc aggacgaaga gcgtgggtgt
    18421 ttgaaaactc gccacaacgg tctgcccttc ttcgacattg cgcgagacga ccgtgccgtt
    18481 caccggagaa aaaatagtgg tgtacccgag gtccaattcg gcggacgcca gtgtggcctt
    18541 ggcctggtcg acctgcgcat cagacacatc cacctgggcc tcggcgtcgc gcgcattggt
    18601 acgtgccaga tccaggtccg cttgggcgac gaactgctcc ttcctcagcg cggccatgcg
    18661 attcagttcc aacgtttttt gcacgagcat gttccgtgct ttggcgagac cggcctgcgc
    18721 gcttttgagc gcggccttag cctggctcac cttggcttga taaggttttt gatcaatgga
    18781 cgcgaggatc tggccctcgc gcacgaccga gttaaagtcg gcatagagtt tggcgatctt
    18841 tcctgagacc tggctgccga cctgcacgga gataaccgga ttgacggctc ccgttgcggt
    18901 gacgagtgcg gtgatcggcc cgcgatcgat cgtcacggtt ttgtagtgac cattaaccgg
    18961 atcattggac caccagtgcc agagcgcagc agccagaagg gtgaggacgc ctaccatccc
    19021 aatgacaatc ggcgcccgac ggcggtggcg aggaagcgtc tgttggctga tgctcgccgg
    19081 ggcacgacca tggccttgac cttgcactac gggaaccgcg ttcacgatga cgggcgtggg
    19141 atgggcggct cgtggaggat cgatgtccga atgatctgag gtcatgtgtt cccttccgtt
    19201 tctctcgggg aagacgcaag agaagtgccg tttcgtttcc ggctgaaaaa tgtgatgagt
    19261 cggaactggc gctgctgagg gccgccggct cgtcatcgat ggtggggcat tttgcaacag
    19321 gttgctggag gagcctgcgg caggacgccc cttgaccgca taggattgtg gctattagtc
    19381 tgtgcccgca gatatccggg aggctggtgt tgcgagcaca tgcgagtgag ggacgaggta
    19441 tgaccgagga atggggcgcg gtattggtgg tagatgacga cgccgacatg cggagtctcc
    19501 tctgcgatgt gctgcagggg cgcgggcatc aatgcacggg cgtcggcggc ggcgacgagg
    19561 cgctcaagga attgagcgag gaagattatg ccgtcgtgct caccgatctg cgcatgaaag
    19621 gcatgcaagg cacggaactg ctcgcggaaa tcaaacagcg gtatcctgat atcggagtga
    19681 tcttgatgac ggccttcgga acggtggaga ccgcggtgga cgcgatgcgc catggggcga
    19741 gcgactatct caccaagccg gtgaagaccg aagagttggt tcgtgtggta gagcgcgcgg
    19801 ttcgtgaagc atcgcttcgg cgcgaggtga gtcgtctgcg gaaggaagtg cacaaggagt
    19861 acagcttcca tcagatcctc ggcaagagca aaccgatgca ggccgtcttc gatttgattc
    19921 gacgggtggc cgacagtccc acgaacgtgc tgatcaccgg agagagcggc accgggaagg
    19981 agttggtggc caaggcgatt cactacaaca gcgaccggcg ggatgcgccg tttgtgccgg
    20041 tcaattgcgc ggcgattccg gaacagctcc tggaaagcga attgttcggc catatgcgag
    20101 gctcgttcac cgatgccaag atggataagc gcggattatt cgaggaagcg cagaagggca
    20161 cgctgttcct cgatgaaatc agcgagttgc cgctcatgtt gcaggcgaag ttactgcggg
    20221 ccattcaaga gcgggaaatc cggcgtgtcg gcgcgaccaa gtccattcct gtcgatgtcc
    20281 ggattattgc ggcgaccaac ttgaacctcg tcgatgaagt gaagaacaag cgattccgtg
    20341 aagattttta ttaccgactg aatgtcatcg agttgcgact gccgccgttg cgggaacggc
    20401 gggaagatat tccgatcctg gtcgatgcgt ttttgaagaa gtgcgccgcc gctcgcggca
    20461 gacaggtcaa ggggctcagc gagccggccc ttgccatgct ggccgactac gcctggccgg
    20521 gcaatgtccg ggaactggag aatgtcatcg agcgcgcggt gacattgagc catggggaca
    20581 tgatcggcgc ggaagacttg cccattcagg ttcagggggc gcgaggagat cggcggattc
    20641 tcgacgaagc ggctgagcga accttgccgc tccatgaagt ggagaaagaa tatatcgtga
    20701 aaatcctgga gaagacgggc ggcaataaat atcaagccgc gcatgtgctg ggtatcgatc
    20761 gcaagaccct ctaccgcaag ctcgccgaga tcgagggcaa accacactca gacgcgtaga
    20821 ttctaggtct caccgtcgtg cggggagcca tcgccgtgca ccctttgtcg caaaaatccc
    20881 tgcttcttga atatgtcggt cctgcgctct tgaccctcgg aatatttctc ctggatctat
    20941 ctgtgtcggc cgaatatacg gtctggttgc tgtatgcgat tcccctggcg ctgacggcgg
    21001 caaactcccg accaggcgcg tccctctatg gcgcgggatt ggtctcgttg ttagtggtga
    21061 ttggcggggt cgcgtcaccc acgggcgttc tgccggtttc cttttgggta aatcggttct
    21121 tgggcatcgg gttcatggca gccatcagct tcctgttcgc gaaccgtcgg aatcattccg
    21181 tgccgacggt tccgccaact atgcctgagc cggcgcacaa ggagtcgaca ccaaccgaga
    21241 ctgtgggggc gatcgcccat gcagaagcgg ccgtggaggg agcagtggcc ggacaacgcc
    21301 gggccgaagc ggaactgcat cgcgacaaat tacgcttcga agggattgtg caatcggcca
    21361 tggatgccat catcacggtc gatgaaagcc agaagatcgt cctgttcaac caggcggcgg
    21421 agaacatgtt ccagtggagc gcggaagaca tgctggggcg gccgttggac cgcctgatgc
    21481 ctgagcggtt tcgtagcgcg catgcggaac atatccggga gttcggccgg tcgggcatca
    21541 ccacgcgcca aatgggagca ttgggggtga tcatggggat tcgctccacc ggcgaagagt
    21601 ttcccatcga ggcggccatt tctcaaatcg gcgtggcggg gacgcggtac tacacggtca
    21661 ttcttcgcga cattacggta cggaaacggc tggagcagga attggcggaa cgcgaagccc
    21721 tcctgcgagc catcatcgaa acggagccgg agtgcgtgaa ggtgctagcc cttgatggca
    21781 cggtgcgaac catgaatgct gcgggactgg ccatgattga ggcagccagc aatgcggaga
    21841 tcgtcggcaa agacatctgt catctggtcg ccgttgaatc gcgaagcgtc tttcgggaca
    21901 ttgtccgtaa agccggggag ggagaggcgg gacgtttgga attccggatc gtcggcctcc
    21961 agggcacgcc gcgctggttg gacacccatg tggtgcccct tcgagcacct gacggaagca
    22021 tcacggcggt gttaggggtg acgcgcgatg tcaccgaatg gaaaaaggcc gagcaactgc
    22081 tgcgccaaag cgaggaacgg tatcggcggc ttctcggggt cttgcctgac gcagtcctcg
    22141 tcgtccgtga taatcgggtg ttcttcgcca acgagcaggg gctacgcttg ttcggcgcca
    22201 cccgggcgga ggaccttcag gggaaatgga tctatgactt gattcaccgc gattatcacg
    22261 aggcggtcgc ggagcgtttc cggcatctgc tcgcaggggg tggcgcagta ccggaggcgg
    22321 aggaacggat cctgcgcctg gacggagcga tggtggatgt ggccgtccga gcggcgcagt
    22381 ttagggatga ggacggagcg ggcatcctgg tggtgttgcg ggacatgaca atgcgcaacg
    22441 tggggcagca acgactgaag gaaagcgagg aacggcttca aagtttactg gaatccatgg
    22501 aggatgtgat ttggtcatct tcgttggatt tgacgacgtt gcactatgtg agtccctccg
    22561 tggggcagat ctatggaagg gcggcggaag aatttgtcgc caggccgtcc ttatggatgg
    22621 aggtcgtgca tgccgaggac cgtgcattgg ctgaacaagc cttgcgggac cttcaggcta
    22681 tcggcgagta cgatgtcgag taccggatcc tgaggcggga cggtgaggtg cggtgggtgc
    22741 atgatcgcgg tcgcatcatc aaggatgaaa ccggtcatcc cgtgcggatc gatggcatcg
    22801 ccagcgacat tactgaacga aaacggctgc aagcgcagct tcggcggacc gagcgtgtgg
    22861 ccgaactcgg gaccgtcgca tccggcatgg cgcacgagat cggaaccccg atgaacgtca
    22921 ttctcggtcg ggccgaatat ctcatggagc ggagcaagga ggagtcggtc aaaaagggac
    22981 tacaaacgat catcagccaa gtcgaacgga tcacccgcgt gatgaaccag cttctcgctt
    23041 ttgcgcggcg gcggccggtc gaacatcgtg cgctcgacct ccgtcagacc atcgaagata
    23101 atctggaaat tttccaggaa cgcttgtctc acagtcacat tacggtggaa acctcatttg
    23161 ccgacgattg tccgtgcgtc catgcggacg ccgatcagat gagccaggtg ctgatcaatc
    23221 tggtcatgaa tgccatccac gccatgccgg aggggggaac gttgcgcgtc atgttggcgc
    23281 ctatgccgga tcgcggtatg gtcacactca cggttgccga taccggacac ggaatgccgc
    23341 aggaggtgat cgcaaaaatc ttcgatccct tcttcacgac caaggagttt ggaaagggga
    23401 ccggcttggg tttgacggtg gtcagaggca ttatcgaaga gcatagcgga accatacagg
    23461 tggaaagcca gccaggagca ggcacgcagt tcaccatccg cttgccgatt catgtcgagc
    23521 cgagccgcaa atcgtagccc ctctcgtttt caactcgatt cccagagcgg aaccgcaagc
    23581 ataacggtcg tcgctgtagc gcttcggggc aacctatcgg agaatgtggg gtctttctca
    23641 ccaacgctac ggaagcttcc ctcctgtttg gaaatgcctg cactgcagaa gctccaatga
    23701 atacaggatc ttagcccgtg tcaggtcgac atgcgcatgg catacacttt gcgatggctg
    23761 ctttgtcatg agtgtcgcgt actggagcct caacgtgaac gagacaaccc cagccgtgct
    23821 actggtggtg gaagacgata aggacatgcg gagcctgctg tgcgatgagt tatgggggga
    23881 agggtatcaa ttgcgggaag ccagcaacgg ggttgagggg ttggaggctg tgatgcgggc
    23941 cgcgccggat ctgatcgtca cggatctgaa aatgccctct gggggcttcg attatgtgca
    24001 tcgactgaga gactgcgtcc ctgcctgtcc catcattgtg atgacggcgt tcggggatgc
    24061 gcggaccaaa gaagaagcga tgaaaagcgg cgccacggcc tatttcgaca agccggtgag
    24121 gctgtcggaa ttaaaggcta cggtgaagcg gcttttgcac cgttccgact cccttccgga
    24181 ggagccgtcg tttcactgaa tacccgcgcc ggacgggcgc tttcggctgg tcgcccccaa
    24241 ccggggaggg ttgtcttcga cccataagcc gtagtacata tagggccttc tctgtgaaga
    24301 aaggcccctg cttgattccc tctgatagaa ggagtgatga gatggccgga tctcatgcac
    24361 cagcaacacc tctttctcct ttcgccgatc ccatccttgc cgctcgtttg gaagcgttga
    24421 gacagttagc tgacggcctg accgaccgcg ttgccgtcat ggaccgggat cttaatgtgg
    24481 tctatgccaa tgatgcggcc tggggaaagt ccgggaagcc gccagcggga gtcaagccgg
    24541 ccaaatgcta tgaatcattc gttcaaaagc aagatccttg cgggacatgc cccgctgagt
    24601 ctgtttttgt gacgggtgaa gtgctgaccg tatcctgcaa tacatcaggt gatgggactg
    24661 cctgtggtat gcaccaggct tttccgctta tttcctcggc gggagaggtg ggttctattc
    24721 ttgtcttgtt tcacaagcgg tctgatccgc cggctattcc agcagcgctc ccccagggca
    24781 agctgggcgc atcacgcccg gaacggacag attcgagatt gggcgattta gtcggtgcga
    24841 gtccggcgat gaatcagctt tttgagatga ttcggttggt ggccgacagt tcggcgacgg
    24901 tgttaatcca aggcgaaagc ggcacgggaa aagagctcgt tgccaggacc atccaccaaa
    24961 ccagctatcg tcgtgacaaa ccctttgtgg tggtggattg cggtgcactg ccggagacct
    25021 tgctggagag tgaactcttc ggccacgtca aaggtgcgtt caccggtgcc gccggcgcca
    25081 agcccggctt gtttgaagaa gccgacggtg gaaccatttt cctggatgag attgcggaca
    25141 cgtccccgac atttcaggcg aagttgctac gggttctgca ggaaggcgaa attaaacgag
    25201 tcggcggtac ccaacccatc aagatcgacg tccgtgtcat ttcggccacc aataaggatt
    25261 tgaccgacct ggtcaaggcc aaggcgttcc gccaagatct ctactatcgt ttggcggtgt
    25321 tgccggtgca cttgcctccg ctacgggaac gacgagcgga tattcccctg ctggtgacca
    25381 agttcatcgc ggattcctgc caccgacatc gccaggagct tcgtcaggtg agcgaagagg
    25441 ccatgcgtgc cttgacggct gcgccctggc cgggaaacgt gcgtgagtta cagcactata
    25501 tcgaacgtgc ggtcgtcacc acgacccatt cagagttgac ctgtacggac ctggtgtcat
    25561 taaaattcca cctagaacct gccgatctgc gtacggtcgg gcgggaggcg gccaagcagg
    25621 ccgaacgcgc cagaattctt caggcgttgc agcagaccgc cgggaatcga atgaaggccg
    25681 cccgtgtcct aaagatcagc cgagccagcc tctataacaa actccacgat ttcggtatcc
    25741 aatagagtca ggcaactgag ttagctgccc cttctcttct tggtcacatt tcggccattc
    25801 caacctttca aattcccaac agaatcagcc ccatgctctt cgtctacgtg tctcttgatg
    25861 catccattgc cccgtatcgc cacagaatcg agcgatctgt cgtctagcga attggacagt
    25921 ctaagtagtt gtaatttcat tgttcatcat cctgtggcgc tcacctgtct aaacgtctag
    25981 aaaaactgta gcgtttcacg ccgcttccga ttgtctgacg agagttcata acctattaat
    26041 aaaactcggt atttatcgcg ttccaggggt atccctaggg agggcacatc aattgcgatg
    26101 ttgggcagtg ctggtgaagg tgcgggtgat taggaccggc ctccgaatga cctgctccct
    26161 cgaactttga ggacatcctt caccagccct ttttcccttt ggagcgtttc gtggagggag
    26221 aaaggcgagg ccggaagagc cggggtcctg cctgacggag gccggtcaca cgaccacgtt
    26281 ttttaaccac tcccgcggaa gggcgcggga ttatcaagga gggaggccga catatgaggt
    26341 cttcagtgca gtcaaagcgc aggcgcatca gtcgcaaagc gatgctgagc ctgcaggcgg
    26401 cgctgctctc gggcagtttg ctcgcggcgc cgtgggtgtt tgcggccggg ccgggcgatc
    26461 agtcgcatga aggacatgcg acgccggtgg ccatgccggg ctggacccag accctgaagg
    26521 gtcagaccgt ggtggaaaac gccatggagg gtcgggctgg caatgcagag aagatggaga
    26581 tgcagcacca tcgcctgatg gagaagttgg agaatcaggc gcagacggat gcgaaggcgc
    26641 agcaaacctc cggtgcattc aacgaaatgt cgatgatgca ccagtatatg ggacaggacg
    26701 gcagcagctt cctgctcgcc tcagattcct ctaagggcga accggtgatg acctctggcg
    26761 ggaagtgccc agccaacgcg ccggtcaaga agtacgacgt ttcgatgatc aacatcgaaa
    26821 tcagcttgaa tcggtggttg gacttctatc ccgggtatat gtacgtccat accgcggata
    26881 tcgacaaggc tcgcgccgag gaagcgaaga acaaggaggc gcgcgaaaag gacggctttg
    26941 atcctggtgc ggtcacgacc ggaaaccagg gcgatgtcat tcagccgctg gtccttcgtg
    27001 cgaaccaggg tgactgcgtc aagatgacgc tgcgcaatca gatggaaggc gaggatggca
    27061 gcttggtcat ccaggcctcc agcatgattg tgagctcgac gggtaagcct gcgacgacga
    27121 cgaacccgga taccgtggtg tccccgggca aggcgcagga gttcgaatgg tacatccatc
    27181 cgaacatgca ggaaggtgtg cgtcagttcc actcctggag ccatgatc
//
LOCUS       DAOQEW010000029        26424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_424_length_26424_cov_5.111798, whole genome shotgun sequence.
ACCESSION   DAOQEW010000029 DAOQEW010000000
VERSION     DAOQEW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26424
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_26424_cov_5.111798"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLS35_05175"
     CDS             complement(<1..607)
                     /locus_tag="PLS35_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18079.1"
                     /translation="MSRKEEEEEEERATTVVAIVSNNYLLRLGLQRIVEQEKWLRLIG
                     QTANVTTFDDVIARERPQIMIIDTEIESDVPGLIQKIKATAPETKTILLTGMDQAECT
                     RQAFACGVDGLALKIQPSPVLIATIDYLARAAAAPVTFTGSNTAPLNKPHRPTIHTAI
                     ATNPAQSKWPDGLTEREQEVVRLVSEGLSNKDIANRLCISSI"
     gene            1683..3416
                     /locus_tag="PLS35_05180"
     CDS             1683..3416
                     /locus_tag="PLS35_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNI18080.1"
                     /translation="MKAVIMDGAPWAGTMLAFPVGNELLIDHLLSAVKRLGIREVAVV
                     SCDQDSYVRDLVCRAGHSYGLTVLWRTEQVYRGTAGSLQAVEDFLDTPTFLGIHANVY
                     LRDVDLAAAFKAHDEHRADITLVTQRMAGTDDDLESVEVDPAGLVSRVNILHWSRNRR
                     SQLTPCGLYVFSREVLSQIPRDEYFDVNEQLVPLMRNRGGVIRAHQIEGGVHTIVGPD
                     DFLWLNREILHKDIQGSGHGNGRLGGVEDIVIGENSEISPHSFLLGPLVIGPNCVVED
                     YVHLIGPAVIGPTSYLEKGSLVRESVLRSGTRVQGNARVEYSVIAGDETVPEGMRLRS
                     AFWSTAKPALYEPHGVPAQVSSNRMLQRSRYSQRPARRMQGGTGQRSIYGMVKSVVDV
                     TGAAVGLLFAGPLMLLIACAVKLDSPGPVFFSQKRCGKNGKEFWMHKFRTMVPDAEQR
                     QKDLKAQNSVDGPMFKLQEDPRITRMGKLLRKTSLDELPQLLNVLRGEMSLVGPRPLA
                     YDEMKFCPTWRDARLSVAPGLTGLWQVKARNRNRFSEWIRYDLEYVRTHSLSLDLYIL
                     IRTARVLLKGV"
     gene            3416..5650
                     /locus_tag="PLS35_05185"
     CDS             3416..5650
                     /locus_tag="PLS35_05185"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNI18081.1"
                     /translation="MNVPYTTGLPDVDSTQTVGRSSFGVREHLITIFRQKRKLLTILG
                     MATLVAGPGSFLMTNIYSAEVRLLIQNARTPFSMSTPLTGQVFTPSDITQKDDVATEV
                     QIFTSPILLDKLVDYFGNERVLAAMRGRWDWVGEIPKTVFKQLSGLPPVAKLLTTIGY
                     VPKPEDLHYQAVQKIRAHLNVEGVRQTHVFVASLDSPDPEFSADALNALVGIYMDHQL
                     SVRKGKGAREFFDEQTKQMRGELQQAELRLQAFKDKWNIVSIEDQKRHLLQQVTHTEA
                     ALRESQVQVAETEVRISKLKERLAGQQEAIPLTNVSERNPMLDQLKNRLMQMELEYSQ
                     YVPDSPAAAELVREIAAVRARLQAESSKVTGAATSGLNQNYQELKRTLVMEEGRRESL
                     RPRLSELTKQFKSYRDSLNTLDQREMELQGLMREVKVKQEAFDIYLKKEEESRINEVL
                     DRKGISNVNAIEHATAPQKPVQPRKFLNLVIGLLIGLVGGFGSAYVSEYMRRTFVTRE
                     EAEDNLGRPVLAALPLVRPDTSDAGSFDLELRQAAQHVIRSYHERGLRTLLVTSALRG
                     EGRSHVAGALARVLAEQKFRVLLITFEEAGAEKLNTGDAAAGAKQATDTGRFRQDIPE
                     ATDRIHLHRLLVRHREGTALEFAEHLAEVAKTMRDRFDLIVIDGPALTSFPEMRVAIA
                     GIDGTILVVEAERTVSVAAARTTTAIEAAGGHLLGLVLNKRRYIIPEWIYGRWLAAGG
                     REGV"
     gene            5647..6441
                     /locus_tag="PLS35_05190"
     CDS             5647..6441
                     /locus_tag="PLS35_05190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI18082.1"
                     /translation="MSESIAGVMQVDEKIVPAEGFVREDGWYAPYDYWLTLWGLYDLY
                     AYALALLGDVKGKVLLDCGCGPGHTSVMLAKRGAIVTAFDTADGQLDTARRLAQANGV
                     NVTFLCKPFEELDFPDESFDVVFGTFVLHHVDLPKASQQLARVLKPGGKAVFIENSAL
                     NPLLMAARSKVCGRLGVPQYSDDHEHPLTRRDIATLQSAFPGACTIHFPSFLFFRLLD
                     FYVFKKRWSWMTALLRHCDQLAGSIPIVRRYGYLQIVELGKGQGRA"
     gene            6434..7708
                     /locus_tag="PLS35_05195"
     CDS             6434..7708
                     /locus_tag="PLS35_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNI18083.1"
                     /translation="MRSKPSKFGRGFCCALLLLSGLFLVATDARSAPATEAVAGCRTE
                     APALKHHRASAVDRVTLTSPSNGVYIGLYSIGTIPEDRLRFTAKSGHRPAIVFTFHDW
                     VSDDDWTASSPHFRTFTDPLESSRLSPLQLAEQVRSEGGVLAVAWAIQCCDWESHLFW
                     YGFRKPTVTVDRLLRGDFDSYITSVARQIKAYRHPIMLTLFSEFNYQGMMGFGKEGRT
                     RLDDVDHVCRLYGDPTWPDGPERIRDAFIYVIDRFRNEDVRNVTWFMYAGSHYMNPQH
                     EDYSPWLHPQYFYPGDDYIDWVGQSAYFIDTRSVPKLRQQELATSFAGALGPGYAAWG
                     AVTDRPLFLPEFGVLGDKTSSRARVIEEVFRDVLPQFPRVRAITLADFKIAEDYYEVP
                     RFGTFEDETEAWKRAVRQNPRYLKQASFKGGE"
     gene            7716..9092
                     /locus_tag="PLS35_05200"
     CDS             7716..9092
                     /locus_tag="PLS35_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNI18084.1"
                     /translation="MALKRSSQWLCLCAIFAVCFTLRIGVGGVIDATVSVVKRADVYS
                     DIAVNLARGHGFVVVAGGEPIIWRAPLYPAFLAQVYALFGEYSETGVFLAQSALDSFT
                     AVLIFYMGTRLFSETVGLLSAVSFALHPLSAYYSLRFLSEPLFTLAFTALISAWIAAT
                     TTRRLMAYTVVGALIAVAALVKPVALGLWPLLATCAVWQLRDEPVRALSAVTGLTLAC
                     LMVLAPWALRNYRATGEVVAVATGGGYALWLGNQMVSEGKEDWEVDEVTRAHLFERRG
                     TVITGAQPSDHALAGYPSIRSATQPVHISVPEDHAFFIAAWREMSSHPFDTALLTVRK
                     FFRFWFRIFLPDNRWAQSYIVVFQTLFLSVAVLGMLEAKRQGITVFALILPVLFLAAV
                     HAMTFSTIRYSIPTIPVLSILQAAGLRAIVHALNERWGLSLGVSWVRWLKAAPVGSAA
                     RSYRGTAL"
     gene            9139..10368
                     /locus_tag="PLS35_05205"
     CDS             9139..10368
                     /locus_tag="PLS35_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI18085.1"
                     /translation="MKPIRVVIIRNCRGITGMTGGETYLLSLLKGFDPQKVRCLLVCI
                     VNPALGETSWLRELKQTGFEHVTIPIGNPFNLSDVYKVSSLIRSYEADVVHALDHRSD
                     LIGILSARLTGRPVLASFLGWVNFPKGSWRATVYPWIDRMILKRLDAVITDSVAIGTE
                     LKMREQDPPVVAIRNGIDTRWFDPTRTLTPFSQESFLKGGHFVFGMVGRIHPVKGHLN
                     FLKAARTLLETHADCRFLIVGTVLPGFEHYRQGLQDFIREQGLEQSVLITHVSLAEIP
                     AVFAGIDVLVAPSFAESFSFTLLEGMAMEKAVVSSEVGGAGEMITHGETGYLIPVDDV
                     SRLTETMRMLRAAPEKVREVGLRARKKIVNDLGIEAMAARTHQVYRSLIEFKGKTSGG
                     AGDQGRLREQLAMISAK"
     gene            10405..11361
                     /locus_tag="PLS35_05210"
     CDS             10405..11361
                     /locus_tag="PLS35_05210"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI18086.1"
                     /translation="MKILITGSQGFLGKSLAHYAVRNGHHVLGVSRSDADGLIAGMES
                     IRCGLDQAGFVDVLNGFAPDVVVHAAGSASVGYSFQQPREDFLMAVETWASVLDAVRK
                     AEGNPLVIFPSSASVYGNPTRLPVPEGAGLRPISPYGFHKVICEQLAQEYAHCFGLNV
                     VSARLFSTIGPFQQRLLVWELFRQAIDANPYLTIQGTGTETRDFLHIDDICRYFLGIA
                     EREPKGFWAVNVASGESTSVRTMAEMVTRLVGSTQAIVTLNKDLPGDPKQWQADTTLL
                     QELVPYQAPDIANGLEDCVRHWLSEPSLSAQRELRCSVEGHS"
     gene            11467..12774
                     /locus_tag="PLS35_05215"
     CDS             11467..12774
                     /locus_tag="PLS35_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNI18087.1"
                     /translation="MQVLRSSMKALSQQTAVVFAGKMTGACLGFVMSLLVARWMGPEE
                     FGLFSLFIVILIFGNDVLGDGLSPGVVRFYSMYRRADPSKAAEVLTNALALRMLLGIP
                     VVVVGVAVGSQYAARVFHSQLYVLPVVLGLVGSFGAALWSFNLSVWQAREEFGTYGVM
                     VALINVLRVLSLPLLFLGGYLTLGGIMGSHVLVYFLCALSGTWALRRHLAHVRVNGEL
                     LRELFRFSKWPAMASLCFILQVNLGVPVLNYFVNAREAGLYGAGSSLLMGVDFLTVSL
                     LTTLLPKVSQLTGIEQCRSYVQRSFPMYALIAAAILPLLFFARPVVIGLFGSAYEGTI
                     EVFQVLFVGTLGTLVTHPLYLVLYTMNRPHLHTLSGIVGLIVWLLAGFWLIPQFGAVG
                     AAWTTLCSRLVQSLMIVVILWHALGLGGSSGLARSVMPDVRGS"
     gene            12771..14267
                     /locus_tag="PLS35_05220"
     CDS             12771..14267
                     /locus_tag="PLS35_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI18088.1"
                     /translation="MKPSDQTYTIPTLLGATLGASVLALSTKALAIVIACSAFLSMTA
                     SAVFPYYILVATIPVQVDLLGGLTVTKLITPLAIGMVTINALIHRGPWPVLLRWPAGY
                     LAGAFFLASLLSLVLAEGLRGLPGEAAKIPVYGALFFFTLTFNRHPEDMRRLLWVITI
                     TGVGEALITAAQVHYGFVMPGDWRRNVGLPSEGGVDGAFMSISEGKVRAEGTTAHPIF
                     LASFFLMSMPCTAFLLFQEKHPWVKAALGAMLSLMAYAWFYTFARSSVIGFALLILVG
                     LWLQFKFVRKLSLIVLVLVLAGGLSYQVVSEWFQVGVQTVENQNLFGKADLNEGSGSF
                     AFRRESIVGGWNLFLAHPWFGVGFGQAINHYVKYLPAWADHYYHPAVIHNMFLEVASE
                     LGVFSLSAFIGLWVVAFVSLKRGLRIPDVRPYAVLLSSILAGQMIFLMITPMVREIWL
                     TLPMAIAIGHMAKTHTRATFGIPFRQVGARAQLRRIVDRGNGLPAGLR"
     gene            14275..15279
                     /locus_tag="PLS35_05225"
     CDS             14275..15279
                     /locus_tag="PLS35_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18089.1"
                     /translation="MKIRVSVVIPAYNATKTIGDGLESFARQSFPAAEVEIIIVDDGS
                     TDGTPEYLETYARSWGLAQPRLRVMRQAHQGPAAARNLGAAAAQGDFLLFTDADCVPH
                     VDWIKEMVAPFALSAIAAVKGAYKTKQRSLVARFAQAEFEARYRQLAATEYVDVVFSY
                     SAGFRREVFRSIGGFDTSFPVADNEDTDLSYRVATAGYKIKFNPAAIIYHQHPATLKQ
                     YLRKKHSRAYWRVMVYKRFPGKAIRDSYTPQTLKLQIGSIVLAAGAIAFVPFVPHAMY
                     VAALAGGLFTATALPFLWQLPREDPGLRLVAPFLLVCRAAVMASGLMRTVPLLMSKTN
                     "
     gene            15309..15911
                     /locus_tag="PLS35_05230"
     CDS             15309..15911
                     /locus_tag="PLS35_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNI18090.1"
                     /translation="MRRILHTSWAVCFMVLCLVTTVQASETEAVLTPSVDAGWSGPFR
                     IGAGDVLNVFIWKHKELSTIVTVRPDGKINYPLIGEIEAKGLTLGEIEERINKQLKQH
                     IQDPQVTVILEATHSFRIFVLGEVMQPGVFDLKGPVTVIQALAMARGLTTFASRNKIF
                     IVNPARGNDQRIPFSYSKFVQGEDTNQNVMLRPGDTVIVP"
     gene            16104..17306
                     /locus_tag="PLS35_05235"
     CDS             16104..17306
                     /locus_tag="PLS35_05235"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI18091.1"
                     /translation="MTSPMLFAQESDNSEFVRPQREFGELPSWQNQVSYDRMPASEKL
                     LLGAPIRRDGFTPRLTVTEEYTDNVFFTAKNRGTDYITKIAPGLGYRTSGRDGQLSAD
                     YSVESRVYANNSNQNRTVSRQNGELFGLWNLSDRTSLTLFDRFESFQDPTEQQVPGIL
                     GAFGRTSINIGALMMRHRLTPSVDLLANYGNFLWSVDAPGAINSMTHEGEVGARVRET
                     QWGRTTVKYRFRFFDFQQGRDFQSHSALVAQEIDLSETLVVSGTIGAVRVEPNPSTVE
                     VLAQASIKKSIGDALYELSYMRDVFPPSGGLSQPLVGDFVRATAKIRLANNFLFDAGL
                     TWILTSTNSDDLTVHTLKWNVGISYTPASWLVARLGYDMFDQREDILGTSASRLANKV
                     SLRMIATF"
     gene            17378..18529
                     /locus_tag="PLS35_05240"
     CDS             17378..18529
                     /locus_tag="PLS35_05240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI18092.1"
                     /translation="MEPVSDGLRILFIAPAPRAGTVQYTHNLANALVDRGHRVTLVTG
                     VGFELADYPRKYTALEVFDRYRPRPLRLCRFVWHCLTFRPQIIHLQGAQHPALYILLW
                     AMLRLLGSASFVYTPQDVLPNSLRSYHIRAFRFLYARMRHVFLNAKQNEPLVIEHFAV
                     PRDRITVLPIADLTAFVREHVAGESPDIPAASRVVLCFGLIEPRKGIHTLLSAAPEVL
                     RQVPDALLLIVGKPLMDIAPLQRQLADLGLQGRVRLVPQYVSFAQMAGYFTAARLLVL
                     PYENGWNSGVLASAFGFGKPVIATRVGGFDEVVSDESTGLLVPPKDPAVLAQAIVRLL
                     RDDALYARMVVNVRQAAAEISWETIARMTEARYGDVVGIGADCAVSQAR"
     gene            18507..19373
                     /locus_tag="PLS35_05245"
     CDS             18507..19373
                     /locus_tag="PLS35_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI18093.1"
                     /translation="MQYRKLGNTGLTPSVLGFGCSMIASLATRYSPSEVEASLREARE
                     AGITFFDTADVYGQGDSERLLGKLFRRGGEGMILCTKAGLTVGSMEPVVRVVKPVMNS
                     ILRRWRTARVATTQARRRQEQQCFDPDYLSGRIEGSLRRLSVDRIDLFLLHNPPVDLP
                     QREGVFELLTRFKAQGKLRWFGVSCRSLDDAYEWLGQPDVACVQIPVDRSRIEAAIPV
                     LERARAQQVGVIAREVFAQDALAKGSVSDALGPLVERAEIGVVLAGMSCRRHVQENVR
                     ALDVAMRCSHVA"
     gene            19363..21021
                     /locus_tag="PLS35_05250"
     CDS             19363..21021
                     /locus_tag="PLS35_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNI18094.1"
                     /translation="MLLDARSISNGAILTADVCIIGAGAAGITLAMELLATGLNIILL
                     EAGGKSKAGESQSLYQGELNDPVRHLPLDQARYRQLGGTTSLWGGRCIPFDSLDFDQR
                     EWISHSGWPFPQKEFDGYYRRAHSYCECGEYDYRVSTALPGAPPSMLPGFEDGDVNTS
                     GIERWSPPTQFGKVYRPVLTRADNLRVLLHALAVELQTSSDGKRIDSVDVATFSGPRF
                     TVRARTTVLAGGGLETTRLLLASRGVHREGIGNHSDWLGRGYMSHIHGVIASVTLTAG
                     QDVMFGYEADPQGVFCRRRIAFSEEAQRRHRLLNLYMLLDRPLVGDPGHGNAVLSAAF
                     LLKRLLGGRQQEELGKGKYSLYWRHIKNILAGSPQALSVLPKFGRKRLLQRRRIPSLL
                     MRSRSNTYYLYFQSEQVPHWENRVTLDAARDEFGTPRLRLHFEVMEQDRQSVQRAHEL
                     IDQELRRQNCGYVTYLGNDVSQLMRDVKAVLGHHIGTTRMSADPSQGVVDAQCRVHGV
                     ANLFVASSSVFPTSSHANPTLTIVAMALRLADHLKALHSGVTRV"
     gene            21052..22062
                     /locus_tag="PLS35_05255"
     CDS             21052..22062
                     /locus_tag="PLS35_05255"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNI18095.1"
                     /translation="MYWKNKRVLVTGGAGQIGSHLVARLAAAGAQVTVADNLWRGKKS
                     NLLGDDGAPVIDMESRFLELDLCDYRNCERATEGQDIVYHLADVVAGINYVFGNQFSL
                     FNTNLIIDSNMLRAAIANKVSSYVYVGTACSYPAERQSKLNPPPFKEDDVYPASPESS
                     YGWSKLMGEMGCDLARKEKLLDIGILRFHNVYGPHCEMSPEKSQVIPSLIRKAVRHPD
                     EEFVVWGSGKQRRAFVFVSDIVDALVSVATKGLNQGVIQIGPDYSTSIKEIAELVCAI
                     SGKPIDIRFDTSKPEGDVDRAADWTRARDILGWSPKTSIQEGLERTYAWCDRQVFQGV
                     GR"
     gene            22168..23175
                     /gene="gmd"
                     /locus_tag="PLS35_05260"
     CDS             22168..23175
                     /gene="gmd"
                     /locus_tag="PLS35_05260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNI18096.1"
                     /translation="MKKALITGISGQDGSYLAELLLAKGYEVHGIIRRSSSFNTGRID
                     AIYQDPHVAEPRLRLVYGDLNDASSLNKILRTIQPDEIYNLGAQSHVRVSFDVPEYTA
                     EVTALGTVRLLEAIRESGIQPKFYQASSSEMFGKVQEIPQRESTPFYPRSPYGAAKVY
                     AHWITVNYREAYNLFACSGILFNHESPRRGETFVTRKITRAAARIKLGVQQELYLGNL
                     DAKRDWGFAGDYVQAMWMMLQADQPDDYVIATGETHTVREFLDRTFSYLDLDWKQYVK
                     IDPRYYRPTEVDLLIGDATKAKKVLGWEPKVSFEDLITMMVEGDLRAERSILDGTRGA
                     R"
     gene            23178..24137
                     /locus_tag="PLS35_05265"
     CDS             23178..24137
                     /locus_tag="PLS35_05265"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNI18097.1"
                     /translation="MSFWKDKRVVVTGGAGFLGTVVVDQLRKLGCRNIVVPRSHDYDL
                     VDMGDVKRLYVDAAPDLVLHLAARVGGIGANQTHAGQFFYDNLMMGTQLIEVGRQQGL
                     KKFVAVGTICSYPKYTPVPFREEDLWSGYPEETNAPYGLAKKMMLVQAQAYRQQYGFN
                     AVVLFPVNLYGPGDNFDMQTSHVIPALIRKCVEAKERGDDRVVLWGDGSPSREFLYVT
                     DAARAILLAAEHYNGNEPVNLGTGQEITIQALAHVIADAVGFTGEIVWDTTKPNGQPR
                     RCLEVSRAKQLFGFEASTAFVDGIQQTVAWFYANRGSIREVVF"
     gene            complement(24157..25548)
                     /locus_tag="PLS35_05270"
     CDS             complement(24157..25548)
                     /locus_tag="PLS35_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18098.1"
                     /translation="MKIHYAAVLALSFIAGIWISFDASTAEAQSRFSRKRQVQTQSVT
                     PPTTQTTAARDYSILKIVPPPAGGLYMGQYEWVQNDIASFESASGRKTALWSKYRGSW
                     GNGYDAAGQPHLDVQAANLAWQEGKVIVVQAYNTHPAPGESEAPQGFTVDKLLNGVYD
                     AELRRFAAELRQYGKPVFFIAGREPNGIGADYFGGFGPAGDQSLRWAIENKRGFSEFN
                     PSSLPYASLYSDIGTPQVCDGTERLKAAQRYYYDFFVRREGLKFLTFDTMGWSVNQVN
                     QIEYDVNELPSTIDKAYARQLLESCHAFSNFYAGDQYIDWISLNFYMIDYYAKDWPGM
                     TQDYVIPIENHFAALDKVMQEVKTVAPTKPVFFMELGFPDGMSQNSSWAAQKIATGIP
                     RIISGYPQIHGFAMWSAHPSWLIPGVFPYDCLIRPGTQQGTALKNVVTTTPSAFLSCV
                     YLSDGKLHPNCMN"
     gene            complement(25755..26123)
                     /locus_tag="PLS35_05275"
     CDS             complement(25755..26123)
                     /locus_tag="PLS35_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18099.1"
                     /translation="MMTRSWTFGLMAILIGITACSHFQNREAAYLDSVKGRATQTEVR
                     QELGAPKAVRKSDEGETVWVYEIREQQAGNRYTAPGIWCEEYLLTFDGAAVLQKWKQV
                     SHFHGGELMPTECIPGMEKP"
BASE COUNT         5384 a         6660 c         8015 g         6365 t
ORIGIN      
        1 tgatgctcga gatgcatagg cggttagcga tgtctttgtt ggacagcccc tcacttacga
       61 ggcgcacgac ttcctgttca cgttcagtga gtccgtccgg ccacttggac tgcgctggat
      121 ttgtagcgat tgctgtatga atagtagggc ggtgaggctt attcaaaggt gccgtgttgc
      181 taccggtaaa tgttacaggt gctgctgccg ctcgagcaag gtagtcaatc gtggcgatca
      241 aaacgggaga aggctgaatt tttagcgcta ggccatcgac tccgcaggca aaagcttgtc
      301 gtgtgcactc cgcttggtcc attccggtca agagaatagt tttggtctct ggagctgttg
      361 ctttgatctt ttgaatgagc cccggaacat cgctctcgat ctccgtatcg ataatcatta
      421 tttggggccg ctcgcgcgca atcacgtcgt cgaatgttgt tacattggcg gtttgcccga
      481 tgagccggag ccacttttcc tgttctacaa ttctttgcaa gccgagccgc aagagataat
      541 tattgctgac aatggccacc accgtggtag ctcgttcttc ttcttcttct tcttcttttc
      601 tactcatcaa ctctcctgac tgaaactcac ccactggaac ctccacatgc aaggagtact
      661 tagtcgcagt ttgtgaaaat actacgcagt gagcgctagg tttaaaatgt gatggagcga
      721 tcctgcatgg gcgcataatc tcacaccagt tatgcgccaa tcaattggga aattctaacc
      781 agcaatcctg gcaaaacctt acggggaggc gctttccggc gatcctgaac gaggggatga
      841 acaggataat ctggtgcaca taagtgtgca ctctcatcct acgagtccac atactcgtgt
      901 gttccctagg cagtctaagc cggacgttac gtgcatagcc gcggaatgcc aaaggaatta
      961 ttgctggcat ttttaccgca ccacatcctt ggacgcttat cggcactttg atcggcacaa
     1021 aagtacgggt atcacacgta tcgtttcgta aagcgaaagg caccaatgtc cgatgtgtgg
     1081 tgcatcctca cccctgtata cgtgtctccc gcaggtagca ctaggttggg cggcttcggg
     1141 gaaggcgcgt tgaagaaaat gtttagcctg gtcttgccct cgccgatcaa ttttcgacga
     1201 tagcgccaca tcggacaata cattttcccg gataccgttg acggatgatt tctcgattca
     1261 cggaagagaa gaaacaacaa atcctgaagg aggggcgctc caaagggggg atccagcatg
     1321 tatgccgaaa atacggcatt tccgtcagca cgttttatcg atggagagcg aaacagggtt
     1381 cccgagtggt agatcccgcc ctccatctga ggtctttgca aatcgagaac cgacgactga
     1441 agcatcgagt cgccgagctg tcattagatt acaacattct tcgatcagca ctaatcagcg
     1501 atcgtggaag tgaatgttaa ctcgtgccga atctccgatg gctattggtt tacggctatg
     1561 aaatcgtcaa ggaatgacgg tcggagaaca gtgcggcatt tggcccaggt tttatttcgg
     1621 cggattcctt tgcaaggctc ggtcgcaatc gcgccattga gattgagtat ggagcaaatc
     1681 acatgaaagc cgtaatcatg gacggtgctc cgtgggcagg aaccatgctg gcgtttcctg
     1741 tcggaaacga attgctaatt gatcatttgc tctcagcggt aaagcggctg ggcattcgcg
     1801 aggtcgccgt cgtgtcctgt gaccaagatt cctacgttcg tgacttggtg tgccgcgccg
     1861 ggcattcgta tggcctgacg gtgttgtggc gtactgagca agtctatcgg ggcactgcgg
     1921 gtagtttgca ggcggttgaa gactttctcg atacgccgac gtttctaggc attcacgcta
     1981 atgtctattt gcgcgacgtg gatttggcgg ccgcattcaa ggcgcatgat gagcatcgcg
     2041 cagatatcac gcttgtcact cagcggatgg ccggtactga tgacgatctc gaaagtgtgg
     2101 aggttgatcc tgcgggcctt gtttctaggg tcaatatttt gcattggtcg aggaatcgtc
     2161 ggagccagtt gaccccttgt ggtttgtacg tcttcagccg ggaggtccta tcacagattc
     2221 cccgcgatga gtattttgat gtcaatgagc aactcgtgcc gctgatgcga aaccgtggag
     2281 gtgtgattcg tgctcatcag atcgagggcg gcgtacatac cattgtcggg cctgacgact
     2341 ttctttggct aaaccgcgag atcttgcaca aggatataca gggcagtggg catgggaacg
     2401 gtcggctcgg tggggtggag gatattgtga ttggggaaaa ttccgagatt tccccccact
     2461 catttctgct tggcccgctg gtgatcggcc ccaattgtgt ggtggaagac tatgtgcatt
     2521 tgattggccc agctgtgatc ggtccaacct cgtatttgga aaagggaagt ctcgtgcgag
     2581 agagcgtcct caggtcgggg acaagggtgc aagggaatgc cagggtcgag tactccgtga
     2641 ttgccggaga cgagacagtt cctgagggca tgcgtctgcg aagtgcgttt tggagcaccg
     2701 cgaagccggc tctctatgag ccgcatggag tccccgcgca ggtctcctcg aacagaatgc
     2761 tacaacgatc aaggtattct caacggcctg caagaaggat gcagggcgga accgggcaac
     2821 ggtcgatcta cggcatggtc aagtccgtag tcgatgtcac cggggccgca gttggactac
     2881 tcttcgcggg ccccctgatg cttctcattg cgtgtgcggt caaattggat tctcccggtc
     2941 ccgtattttt ctctcaaaag cgctgcggga agaatggcaa ggagttctgg atgcataaat
     3001 tcagaaccat ggtgccggat gcggagcaac gccagaaaga cttgaaggcc caaaatagtg
     3061 tcgatgggcc gatgttcaag ttgcaagaag acccacgtat tacccgcatg ggcaagcttc
     3121 tacgtaagac gagcctcgat gagctgccgc agttgttgaa tgtcttgcgc ggggagatga
     3181 gtctcgttgg gcctcgccct ttggcgtacg acgaaatgaa gttctgtccg acctggcgcg
     3241 atgcaaggct cagtgtggcc cccgggctaa cgggtctttg gcaggtgaag gctcgcaatc
     3301 gtaatcgatt ctcggagtgg attcgatacg acctggagta tgtgcgtacg cattcgttgt
     3361 cgctggatct gtatattctc atccggaccg ctcgggtgtt gctgaaagga gtgtgatgaa
     3421 cgtgccgtac acgactgggc tccccgacgt agactctacg cagactgtcg gtcgatcgtc
     3481 attcggcgtc cgagagcacc tcatcacgat cttccgccaa aagcggaagt tacttacaat
     3541 tttgggaatg gcaacccttg tggcggggcc gggctcattc ttgatgacaa acatctatag
     3601 tgccgaggtt cgattgctca tccagaatgc gcgaaccccg ttcagcatga gcacgccgtt
     3661 gacggggcag gtgttcactc cttccgatat tactcaaaaa gatgacgtcg cgacggaagt
     3721 gcagattttc actagcccga ttctgttgga taagctggtt gactacttcg gaaatgaacg
     3781 agtgcttgct gccatgcgag ggcggtggga ctgggtcggt gaaatcccga aaaccgtttt
     3841 caaacagttg tccgggttgc cgccggtggc caagttattg acgacgatcg ggtatgttcc
     3901 caagccggag gatctgcact atcaagccgt gcagaaaatt cgggcgcatt tgaatgtgga
     3961 aggagtgcgg caaacgcacg tatttgtggc cagcctcgat tcccctgatc cagagttttc
     4021 cgcagatgcg ctcaatgcgc tggtcggcat ctatatggat caccagctat cagtccgcaa
     4081 aggcaagggt gcgcgcgaat tctttgacga gcagacgaag cagatgcgcg gcgagttaca
     4141 gcaggcagaa ctccggttac aggcctttaa agacaaatgg aacattgtct ccatcgaaga
     4201 tcaaaagcgg catctcctgc aacaagtgac gcacaccgaa gccgcacttc gtgagagtca
     4261 ggtgcaggtc gcggaaacgg aagtgcgcat cagcaaactc aaggaacgcc tggctgggca
     4321 acaggaagcg attccgctta cgaacgtctc ggagcgaaat cccatgctgg atcaactcaa
     4381 gaaccggttg atgcagatgg agttggagta ttcccagtat gtccccgaca gtccggctgc
     4441 cgcagaactt gtgcgagaaa ttgccgcggt gcgtgcgcgc ctgcaggcgg agagttcaaa
     4501 ggtcaccggc gcagccacat ccggcctgaa tcaaaactat caagaattga aacgcaccct
     4561 ggtcatggaa gaggggcgtc gagaaagctt gcggccgcgc ctttccgagc tgaccaagca
     4621 attcaaatcc taccgagatt ctctcaatac cctggatcag cgagaaatgg aattgcaggg
     4681 actgatgcgg gaggtcaagg tcaagcagga agcgttcgac atctacctta agaaggagga
     4741 agagtcgcgg atcaatgaag tgcttgatcg caaggggatt tcgaacgtca atgcaattga
     4801 gcatgcaaca gctccccaga agcctgtaca gccacgtaaa tttctcaatc tcgtgatcgg
     4861 tctgttgatc gggttggtgg ggggattcgg gtcggcctat gtatcggagt atatgcgtcg
     4921 aacgttcgtt acccgcgagg aggcggaaga taacctcggg cggcctgtcc ttgctgcctt
     4981 accgctcgtc cgtccagaca cgtccgatgc cgggagtttt gacttggaac tccgccaagc
     5041 tgcacagcac gtcattcgtt cctaccatga gcgtggtctg agaacattgc ttgtcacgag
     5101 cgccctccgc ggcgaaggcc gatcacatgt ggccggcgcc ctcgcgcgag tgctggccga
     5161 gcagaagttt cgcgtgctgc ttatcacctt cgaagaagcc ggcgcagaga agctaaacac
     5221 cggggatgcc gctgcggggg ctaagcaagc gacggatacc ggccggttcc ggcaggatat
     5281 tccagaggca accgatcgta tccacctcca tcgattactg gttcgccaca gggaaggaac
     5341 ggcgttggaa tttgccgagc atctggcgga ggtcgccaag accatgcgcg atcgtttcga
     5401 tttaatcgtg atcgacggcc ccgcattgac ctcctttcct gaaatgcgcg tggccattgc
     5461 aggcattgat ggcaccattt tggtggtcga ggcggagcgg acggtgtcgg tggcggcggc
     5521 cagaacgacg acggcgatcg aagcggccgg aggacacttg ctgggactcg tgttgaacaa
     5581 gcgtcggtac attattccgg aatggattta cgggcgatgg ttggcggcag gcgggaggga
     5641 gggcgtatga gtgaatcgat agcgggtgta atgcaagtcg atgagaaaat agtccctgcg
     5701 gaggggttcg ttcgagaaga cgggtggtat gcgccgtatg actattggct caccctctgg
     5761 ggactctacg acctctacgc ctatgcgctg gcattgcttg gtgacgtcaa gggcaaggtt
     5821 ctgctggatt gcggctgcgg tcccgggcac acgtccgtga tgctggcgaa acgcggggcg
     5881 atcgtcaccg cgtttgatac ggcggacggt caactcgata cggcgcgaag gctggcacag
     5941 gccaacggtg tcaatgtgac gtttctttgc aagcctttcg aggagttgga ttttccggac
     6001 gagagtttcg acgtcgtctt cggcacattc gtcttgcacc atgtggatct tcccaaggcc
     6061 agccagcaat tggctcgcgt cttgaagccg ggggggaaag cggtgttcat cgaaaactcc
     6121 gcgctcaatc cgctgctcat ggcagccaga agcaaggtct gtggacggtt gggtgtgcct
     6181 caatacagcg acgatcatga gcatccgctt acccgtcgcg acattgcgac gctacagagc
     6241 gcctttcccg gcgcttgcac cattcatttt ccgagtttcc tcttctttcg attgctcgat
     6301 ttctacgtct tcaagaaacg gtggtcgtgg atgacggccc tgcttcgaca ctgcgatcaa
     6361 ctcgccgggt caattccaat cgtgcgtcga tacggctatc tgcagatcgt cgagctggga
     6421 aagggccaag gtcgtgcgta gcaaaccgtc caaattcgga cggggatttt gctgtgcatt
     6481 gttgctcctg tccgggctct tcttggtggc gacggacgcc cggtctgctc ctgcgaccga
     6541 ggcggtggcc ggttgtcgga ccgaggcgcc tgcgttgaaa caccatcgtg cgtccgctgt
     6601 cgatcgtgtc accctgactt cgccctcgaa cggggtttac atcggcttgt atagcatcgg
     6661 aacgattccg gaagaccggt tacggtttac tgcgaagagc gggcatcggc ctgccattgt
     6721 gttcaccttt catgattggg tctccgacga cgattggacc gcatcttcgc cgcatttcag
     6781 gactttcacg gatccgttgg aatcgtcccg gctcagtccg ctccagctgg ccgagcaggt
     6841 cagaagcgag gggggtgtgc tggcggttgc ctgggccatc cagtgttgcg attgggagtc
     6901 ccacctgttc tggtatggat ttcgtaaacc gacggtgacg gttgatcgcc tgcttcgagg
     6961 tgattttgat tcatacatca ccagcgtagc ccgtcagatc aaggcgtaca ggcatccgat
     7021 catgcttacc ctattcagcg aattcaacta tcaggggatg atggggttcg gtaaggaggg
     7081 cagaacgcga ctcgatgatg tcgatcacgt gtgtcgactg tatggagatc cgacatggcc
     7141 ggacgggccg gaacggattc gggacgcctt catctacgtc atcgatcggt ttcgaaatga
     7201 agacgtgcga aacgtgacct ggtttatgta cgcagggtct cattatatga atccccagca
     7261 tgaggactat tccccatggt tacacccaca atatttctat cccggcgacg actacatcga
     7321 ctgggtgggg cagagcgcgt atttcatcga tacccggtcc gttccaaaac ttcgacagca
     7381 ggaactcgct acgtccttcg caggggcatt ggggcctggg tatgccgcgt ggggcgcagt
     7441 gactgatcgg ccgctgtttc tccctgagtt cggagtgttg ggagataaga ccagcagtcg
     7501 tgcccgggta atagaggaag tgttccgcga cgtgttgccg cagttccctc gagtgcgggc
     7561 gatcaccctc gctgatttca aaattgccga ggattactac gaagtgccca ggttcggcac
     7621 cttcgaggat gaaacggaag cctggaagcg ggcggttcgt cagaaccctc ggtacctcaa
     7681 gcaagcctct ttcaaaggcg gagagtaggc cggcaatggc gctgaagcga tcttcgcaat
     7741 ggctgtgtct ctgcgcgatt tttgccgtgt gtttcactct gcgtatcggc gtcggcgggg
     7801 tcatcgacgc gacggtatcg gttgtcaaac gggcggatgt gtactcggac attgcggtga
     7861 atctggcgcg cggccacggt tttgtcgttg tagcgggtgg cgagcctata atttggcgcg
     7921 ctccgttgta tccggcattt ttggcgcagg tctatgcgct cttcggggag tacagtgaaa
     7981 caggagtgtt tcttgcgcag tccgcgttgg attcgttcac cgccgtcttg atcttttata
     8041 tgggcactcg cttgttcagt gaaaccgtcg gattgctctc tgccgtgagt tttgccctgc
     8101 atccgctgtc cgcgtattac tcacttcgat tcctgtctga gccgctgttc accttggcct
     8161 ttacagccct catctcggct tggatcgcgg caacgactac ccgccgtctc atggcctaca
     8221 cggtcgtagg tgcgttgatc gctgtcgcag cgctggtgaa gccggtggct ttgggcttgt
     8281 ggcccttgct tgccacctgc gccgtatggc agttgcggga cgaaccggtt cgcgcactat
     8341 ctgctgtgac cgggctgacc cttgcttgtc tgatggtgtt ggctccttgg gctcttcgaa
     8401 actatcgcgc gacgggcgaa gtggtggccg tggcaacggg gggaggctat gctctctggc
     8461 ttggcaatca aatggtaagt gagggcaagg aggactggga agttgatgaa gtgacccggg
     8521 cgcatctgtt cgaacggcgt ggcacggtca tcacaggggc tcaaccgtcg gatcacgctc
     8581 tcgctggtta tccgtcgatc cgatcggcaa cacagccggt tcacatttcg gtccctgaag
     8641 atcatgcatt tttcatagct gcctggcggg aaatgagttc ccatccgttc gacacggcac
     8701 tattgacggt tcggaaattc tttcggttct ggttcaggat ttttcttcct gacaatcgct
     8761 gggcacaatc ctacattgtc gtgtttcaaa ccctcttcct gagcgtggcg gttctgggca
     8821 tgctggaggc gaaacggcag ggaatcacgg tgtttgcgtt gatcctgccg gtcctattcc
     8881 tcgcggccgt tcatgcaatg acattctcca ccattcgcta cagcattccg accattccgg
     8941 tcttgagcat cttgcaggcg gccggcttgc gggcgatcgt gcatgcactg aatgagcgat
     9001 ggggtttgtc gcttggagtg tcctgggtcc gctggttgaa agccgcgccg gttggctcag
     9061 cggcaagatc gtaccggggg accgcgttat gacggggcct gcgatgctga atggctgtgc
     9121 cgagagtagg aaccactgat gaaaccgatt cgagtcgtga tcattcgcaa ctgccgtggg
     9181 attaccggca tgacgggagg agaaacctat cttctttctc tcttgaaggg attcgaccca
     9241 cagaaagttc gttgcctcct ggtatgcatc gtcaatcccg cacttggcga aacgtcttgg
     9301 cttagggaat tgaagcagac cgggtttgag cacgtgacga ttccgatcgg gaatcccttc
     9361 aacctgagcg atgtctataa ggtctcttcc ttgatcagat cgtatgaggc agacgtcgtg
     9421 cacgccctgg accaccgctc ggatctgata gggatactca gtgcgcgtct aacgggacgc
     9481 ccggtgctgg cctcgtttct cggatgggtc aatttcccta aagggtcctg gcgggcgacc
     9541 gtttatccat ggatcgatcg gatgattctc aagcggttgg atgcagtgat taccgattcg
     9601 gtggcgattg gcacagaatt aaagatgcgt gagcaagatc cgcctgtggt cgcgattcga
     9661 aatggcatcg ataccaggtg gttcgatccc acgcgtacgc tcacgccgtt ttctcaggaa
     9721 agctttctca agggtggcca ttttgtgttc ggcatggtgg gccgtattca tccggtgaag
     9781 gggcatctga acttcttaaa agcggcgcga accctgcttg aaacgcacgc agattgtcgg
     9841 tttctcatcg tcgggacggt attgccggga tttgaacact atcggcaggg cctccaggac
     9901 tttatccgcg aacaagggtt ggagcaatcg gtgttgatca cccacgtgtc attggcggag
     9961 atccctgccg tgtttgccgg cattgatgtg ttggttgcgc cgtccttcgc cgagagtttc
    10021 agctttactc tactcgaagg catggcaatg gaaaaagccg tggtgagttc agaggtcgga
    10081 ggggccggtg aaatgatcac tcacggcgaa acagggtatc tgataccggt tgatgacgtc
    10141 tcccggttga ccgaaaccat gcggatgctg agggccgccc cggaaaaagt gcgggaagtg
    10201 ggtctgcgtg cgcgcaagaa aattgtcaac gacctgggga tcgaggcgat ggcagcgcga
    10261 acgcatcaag tgtaccgcag tctaatcgag tttaagggca agacgtcggg cggtgcggga
    10321 gaccaaggtc gattgcgcga gcagctggcc atgatcagcg ccaagtaacc catcgcagga
    10381 actcatgatg gacgtattgt gactatgaaa attctgatta ccggttctca ggggtttctc
    10441 ggcaagagtc ttgctcacta tgccgtcagg aacggacatc atgtgttagg tgtgtcgcgg
    10501 tcggacgccg acggtttgat tgccggaatg gaatctatac gctgcggcct ggaccaggca
    10561 ggctttgtcg acgtactcaa tggttttgct ccggatgtcg tggtccatgc agccggttct
    10621 gcgtcggtgg gatattcgtt tcaacagcca cgagaagatt ttctgatggc agttgaaacc
    10681 tgggcgtcgg tcttggatgc agtgcgcaag gcagagggaa acccgttggt gatctttcca
    10741 tcgagcgctt ccgtctatgg gaatccgact cggctgcctg tacccgaagg tgccgggctt
    10801 cgaccaattt cgccctatgg atttcataag gtcatttgcg agcagttggc gcaagaatat
    10861 gcccactgct tcgggttgaa cgtggtctcg gctcggttgt tctcgaccat cggcccattt
    10921 cagcagcgct tattggtctg ggaactattt cgccaagcga tcgatgccaa cccgtacttg
    10981 accattcagg gcacagggac ggaaacccgt gattttctgc atatcgacga tatttgtcgg
    11041 tatttcctgg gtatcgcaga gcgtgagccg aaaggatttt gggcggtgaa cgtcgcttcc
    11101 ggcgagtcta cgtcggtgag aacgatggcg gaaatggtga cgcgccttgt gggaagtacc
    11161 caggctatcg tcactctgaa taaggatttg ccgggtgatc ccaaacaatg gcaggccgat
    11221 acaacgctgt tgcaagaact cgtgccgtat caggcgccgg acatcgccaa cggcttggag
    11281 gattgtgtgc ggcattggtt gtcggagccg tcgttgtcgg ctcaacggga gctgcgttgt
    11341 agtgtggagg ggcactcctg aacgggcggt acgggataaa gatcacggtg ccgtgacggc
    11401 tagctgctgc cagggtacgg ttgtgtgtgc cggttattca gtgcgaaggg gatggtgtag
    11461 gcggacatgc aggtgttgcg ttcgagtatg aaggcgttgt cgcagcagac cgccgtggtg
    11521 tttgccggaa aaatgaccgg cgcctgtctg gggtttgtga tgagcctgct tgtcgcgcgt
    11581 tggatgggac cggaggagtt cggattattt tccctgttca tcgtgatcct gatcttcggc
    11641 aatgacgtgc ttggcgacgg gttgagccct ggagttgtgc ggttttattc catgtatcgg
    11701 cgtgccgacc cctccaaggc agctgaagtg ctcaccaacg cgcttgcgtt gcgcatgctg
    11761 ctcggcattc cggtggttgt ggtcggtgta gccgtgggat cgcaatatgc ggctagggtg
    11821 tttcatagcc agttgtacgt gctccccgta gtcttgggtc tagtcggctc ctttggtgcg
    11881 gccttgtgga gtttcaacct ctcggtgtgg caggcccgtg aagagttcgg tacctatggg
    11941 gtgatggtcg cgctgataaa tgtgctgagg gtcctcagtc taccgttgtt gttcctggga
    12001 ggatatctca cgctgggcgg aatcatgggg tcccatgttc tcgtctattt cctctgtgcg
    12061 ctgtcgggta cctgggcact gcgcaggcat ctggcacatg ttcgcgttaa cggcgaattg
    12121 ttgcgggaac tgtttcgatt cagcaagtgg ccggcgatgg ccagtctctg ttttatcctc
    12181 caagtgaatc tgggcgttcc ggtccttaac tattttgtga atgcccgtga agccggcctg
    12241 tatggtgccg gatcatcact cttgatggga gtggattttc tgacagtcag cctgctgacg
    12301 acattgcttc ccaaggtcag tcagctgacc gggattgaac agtgtcgttc ctatgttcag
    12361 cgctcatttc cgatgtatgc actcattgcc gctgcaattc ttccgttgct gtttttcgca
    12421 cggccggtgg tcatcggatt gttcggttct gcctatgaag gcacgatcga ggtctttcag
    12481 gttctgtttg tcggaacgtt aggaaccttg gtgacgcatc ctttgtatct agtgctctat
    12541 acgatgaatc gcccgcacct gcacacattg tcaggaattg taggactgat tgtctggctg
    12601 ctagccggct tctggcttat tccgcaattc ggtgccgtcg gagccgcgtg gacgacgttg
    12661 tgctcacgac ttgttcaatc cctcatgatc gttgtcatcc tttggcatgc cctgggattg
    12721 ggcggatcgt cgggcttggc gcgttcggtt atgccggatg tgagaggatc atgaaaccga
    12781 gcgatcagac gtataccatt ccgactctgc tgggcgcgac gctgggcgca tccgtcttgg
    12841 cgctctcgac caaagctctt gctattgtca ttgcctgcag tgcgtttctc agtatgaccg
    12901 cctcggcagt ttttccttat tacatactgg ttgccaccat tcctgtacag gttgatttac
    12961 tgggaggact gacggtcaca aagctgatta ccccgttggc tataggtatg gtgaccatca
    13021 atgcgctgat ccatcgagga ccatggccgg tgctgttacg atggccggcc ggttatcttg
    13081 ccggcgcgtt ctttcttgcc tctctcttgt ctctcgtcct cgcggagggc ttacgtggac
    13141 tgccaggcga ggcagcaaag atccctgttt acggggcgct cttctttttc acacttacat
    13201 ttaatcgcca tccagaggat atgcgccgtc tgctgtgggt catcaccatt accggtgtgg
    13261 gggaggcact cattacagcg gctcaggtcc actacggttt tgtgatgccg ggcgactggc
    13321 ggagaaacgt aggcctgcca agcgaaggcg gtgtagacgg cgcgttcatg tctatttccg
    13381 agggaaaggt tcgtgctgag ggtaccaccg cccatcccat ttttctcgca agtttcttcc
    13441 tgatgtccat gccctgcacc gcattccttc tttttcagga gaaacatcca tgggtcaagg
    13501 ccgcgctggg ggcgatgctg agcctcatgg catatgcgtg gttttacacg tttgctagaa
    13561 gttcggttat cggtttcgcc ctcctgattc tggttggtct gtggttgcaa ttcaaattcg
    13621 tgagaaagct gtctcttatt gtgctggtgc tcgtgctcgc cggaggcttg agttatcagg
    13681 ttgtctcgga atggtttcaa gtcggagtgc aaaccgtaga gaatcaaaac ctcttcggta
    13741 aggcagatct caacgaaggc agcgggagtt ttgcatttcg acgggagtcc attgtgggtg
    13801 gctggaatct ctttttggcg cacccttggt ttggtgtcgg cttcggacag gccatcaatc
    13861 attacgtcaa atatttgcca gcatgggccg accactacta tcaccctgct gtgatccaca
    13921 atatgttcct tgaagtggcg agtgagcttg gcgtgttttc attgtcggca ttcattggtt
    13981 tgtgggtggt ggcgttcgtc agtctgaagc gggggcttcg gattcccgat gtgcgaccct
    14041 acgccgttct gttgagcagc atcctcgcgg ggcaaatgat ttttctgatg atcaccccga
    14101 tggtgcgaga gatttggctg acattgccga tggccatcgc catcggacat atggccaaga
    14161 cccacacgcg ggccacgttc ggcataccct ttcgacaggt gggcgctcgt gctcaactac
    14221 gacggatcgt tgatcgtggg aatggcctgc ctgcaggcct gaggtgagga catcatgaaa
    14281 attcgcgtca gtgtcgtcat tcctgcctac aatgccacga aaaccatcgg cgatggcctg
    14341 gagtcctttg ctcgtcagtc atttccagca gccgaagttg agatcatcat tgtcgatgat
    14401 ggctcgacgg acggcacgcc ggaatatctg gaaacctatg ccaggagctg gggattagcg
    14461 cagcctcgat tgcgtgtgat gcgacaggct catcaaggcc cggcggcagc gaggaacctt
    14521 ggggcggcgg cggcacaagg ggattttctg ttgtttacgg atgccgattg cgtgccgcat
    14581 gtggattgga tcaaggaaat ggtggcgccc ttcgcattgt cggcgattgc cgcggtgaag
    14641 ggggcctaca aaacaaaaca acgcagtctc gtggcgcgct ttgcccaggc cgagtttgag
    14701 gcccgctatc gtcagctcgc tgcgacggaa tatgtcgatg tggtcttttc ctactccgcc
    14761 gggtttcgtc gtgaggtgtt ccgctctatc ggaggattcg acacgagttt ccccgtcgcc
    14821 gataacgaag ataccgatct gtcgtatcgg gtcgccacgg ccggctacaa gatcaagttc
    14881 aatccggcgg cgatcatcta tcatcaacat ccggcgacgc tcaaacagta tctccggaaa
    14941 aaacatagcc gggcgtattg gcgagtgatg gtgtacaagc ggttcccggg caaggccatt
    15001 cgtgattcct acacgccgca aacgctcaag cttcagatcg gctcgatcgt gctggctgcg
    15061 ggggcgatcg cgtttgtacc atttgtgcct catgccatgt atgtggcggc acttgcgggc
    15121 ggactcttca cagccacagc gttgccgttt ctatggcagt tgcctcgaga ggatccggga
    15181 ttacgactgg tcgcgccatt tcttctagtg tgtcgggcgg ctgtcatggc ttcaggtttg
    15241 atgcgaacag tgccgttact catgagcaag accaattgag actcatgtta ctgaggggga
    15301 gacaagacat gcgacgtatt ttgcacacaa gctgggctgt ttgtttcatg gttctgtgtc
    15361 tggtgaccac tgtgcaggca tccgagaccg aggcggtact tactccgtct gtggatgcgg
    15421 gctggagtgg tccgtttcga ataggtgcgg gggatgtcct caatgtattc atctggaagc
    15481 acaaagaact gtcgactatt gtcaccgttc gtcccgacgg aaaaatcaat tatccattga
    15541 tcggcgagat tgaggccaaa ggactcacgc tgggtgagat cgaggaacgg atcaataagc
    15601 aattaaagca gcacatccag gatccacagg ttacggtgat cttggaggcg acgcatagtt
    15661 tccgcatctt tgtcctgggt gaagtgatgc agccgggtgt ctttgatctc aagggaccgg
    15721 tcaccgtcat tcaagcgctg gccatggcac ggggattgac cacgtttgcg tcgcggaaca
    15781 agatttttat cgtcaatcct gcgcgcggta acgaccaacg cattccgttt agttacagca
    15841 agtttgttca gggggaagat acgaatcaga atgtgatgct tcggcccggg gacacggtga
    15901 tcgttccgta gtgtcagtgg aatttcaaca atccgggacg aaagcgaagt tgagtgtcac
    15961 acagaatgga atgccttgtc acatccggga gtcgcgtgag caggcgaggt atcgttttga
    16021 acagtgcacg aggtccgatt gggcggactt tccgctggtt gcgtattgcg gtgcttggaa
    16081 ttgcatcggc tgcaggtgca gtagtgacct cgccgatgct ctttgcgcag gagtcggaca
    16141 actcggagtt tgtccggcca cagcgcgagt tcggtgaatt gccatcctgg cagaatcagg
    16201 tgagttatga ccggatgccg gcgtcggaaa aactgctcct aggtgctccg attcgccgag
    16261 atgggtttac gcctcggctc acggtcactg aagaatatac ggacaacgtc ttcttcacgg
    16321 cgaagaatcg cgggaccgac tacattacga agattgcgcc tggactgggt taccggacgt
    16381 ctgggcgaga cgggcaactc tctgcggatt attcggtcga gtccagggtc tatgcgaaca
    16441 acagcaatca gaacagaacg gtgtcgcggc agaatgggga gctgttcgga ctttggaatt
    16501 tatccgatcg aacgtcgctg accttgtttg atcgctttga atcattccaa gaccccacgg
    16561 aacagcaggt gcccggtatt cttggcgcat ttgggcgcac ctcgatcaat atcggggctt
    16621 tgatgatgag gcatcgactc actccgtccg ttgatctgct agcgaattat ggaaattttc
    16681 tctggtctgt cgatgcgccg ggtgccatca atagtatgac acacgaaggc gaggtgggcg
    16741 cgagagtacg ggagacgcag tgggggcgta ccaccgtaaa gtatcggttt cgattctttg
    16801 atttccagca ggggcgtgat tttcagtccc attctgcgct ggttgcccag gaaatcgacc
    16861 tgtctgagac gctcgttgtg agcgggacga tcggtgcggt tcgtgtggag ccgaatcctt
    16921 cgacggtcga agtcctcgcg caggcgtcga tcaagaagtc gatcggggac gcgttgtatg
    16981 aactgagtta tatgcgcgat gtgtttcctc catcgggagg actcagtcaa ccgctggtcg
    17041 gagattttgt acgggcgacg gcaaagatac gacttgcgaa taactttctc ttcgatgccg
    17101 gactgacgtg gattttgacg agcaccaatt cagatgattt gacggtacac accttgaaat
    17161 ggaacgttgg catttcgtat acgccggcct cctggcttgt ggcccgactg gggtacgaca
    17221 tgttcgacca gcgtgaagac attttgggca cgtccgccag ccgccttgcg aacaaggtca
    17281 gtctgcggat gatcgcgaca ttctgagggt gtgcatacgc tacggaattg atgacatcgg
    17341 gtgagactcc gagtgcagag cagtaaggtg ggtgaaaatg gaaccagtga gtgacggact
    17401 tcgcattctt tttatcgcgc cggctccgcg cgccgggacg gtgcaatata cccacaacct
    17461 ggccaatgcg ctggtggatc gggggcatcg cgtcacgctg gtcacaggtg ttggattcga
    17521 attggccgac tatccgagaa agtatacagc gttagaagta tttgatcggt atcggcccag
    17581 gcccttgcgg ctctgtcggt ttgtctggca ttgcctgact ttccggccgc agattattca
    17641 tctacaaggg gcgcaacatc cggccttgta cattctgctg tgggcgatgt tgcggctctt
    17701 gggcagcgcg tcgttcgtct atacgcctca agacgtcttg cccaattcat tgcgttccta
    17761 tcatatccgc gcgtttcgtt ttctctacgc ccgcatgcgg catgtgtttc tcaatgccaa
    17821 acagaatgag ccgttggtca tcgagcattt tgcggtgccg cgtgaccgga tcacggtgct
    17881 tccgattgcc gatctcacgg cgtttgtgcg ggaacacgtg gccggtgagt cgccggacat
    17941 tccggccgcg tcgcgggtgg tgttgtgctt cggattgatc gagccgagga aagggatcca
    18001 tacgcttctc tctgccgcgc cggaggtgct tcggcaggtg ccggatgctc tgttactgat
    18061 tgtcgggaag ccgctgatgg acattgcgcc gttacagcga cagctggccg atttggggtt
    18121 gcagggacgg gtgcgattgg tgccgcagta cgtgagtttt gcgcagatgg ccgggtattt
    18181 cactgccgcc aggctcttgg tgctgccgta cgaaaacgga tggaatagcg gcgtcttggc
    18241 gtctgctttc ggattcggga aacccgtgat tgccacgcga gtaggcggat tcgacgaggt
    18301 cgtcagcgac gaatcgacgg ggttgctcgt gcccccgaag gatccggcag tgttggcgca
    18361 ggcgatcgtt cgcctgttgc gcgatgatgc gctgtatgcg cgcatggttg tgaatgtccg
    18421 gcaggcagcc gcagaaattt cctgggagac gattgcacgg atgaccgaag ctcggtacgg
    18481 ggacgtcgtg gggattggag cggattgtgc agtatcgcaa gctcggtaat accggtctga
    18541 cgccgtcggt gctgggattc ggatgttcga tgatcgcttc gctggcgaca cggtattcgc
    18601 ccagcgaagt ggaggcgagt cttcgtgagg ccagggaggc ggggattacg ttctttgata
    18661 cggccgacgt ctatggtcag ggtgatagtg aacgtctgct cgggaagctg tttcggcgcg
    18721 ggggcgaagg aatgattctc tgtaccaagg ctgggttgac ggtcgggtcg atggaacctg
    18781 tggtgcgagt ggtcaagccg gtgatgaatt cgatcctgcg gcgttggcgg actgcacgag
    18841 ttgcgacgac gcaggcgcgc cgccgccagg agcagcagtg ttttgacccg gactatcttt
    18901 ccggacgaat cgagggaagc ttgcgccggc tcagcgtgga tcggattgat ctctttctat
    18961 tgcacaatcc tccggttgat cttccgcagc gtgaaggagt gtttgagctg ctgacccgct
    19021 tcaaggcgca aggcaaactg cgttggtttg gcgtgtcctg ccggtcgttg gacgacgcct
    19081 acgaatggct agggcagccc gatgtcgcct gcgtgcaaat tcccgtcgac cgcagtcgaa
    19141 tcgaggcagc aatcccggtg ttggaacgcg cacgagccca acaggtgggc gtgattgcac
    19201 gggaggtatt cgcgcaggat gcactggcca aaggctccgt gtccgatgcg ttaggtccgc
    19261 tggtggaacg tgcggagatt ggcgtcgtgc tcgcgggtat gagttgccgg cggcatgtgc
    19321 aggaaaatgt gcgtgcgctg gacgtcgcga tgaggtgtag ccatgttgct tgatgcgcga
    19381 tcgatttcca acggagccat cctcaccgct gatgtctgca tcataggcgc aggtgccgct
    19441 ggtattaccc tggcgatgga actgctggcc actggtctga acattatcct attggaagcc
    19501 ggcgggaagt caaaagccgg agagagtcag agcctctacc agggagagtt gaacgatccg
    19561 gtccgacact tgcctttgga tcaggctcgg tatcgccaac tcggtggcac gacatctctg
    19621 tgggggggac gctgtattcc gttcgattcc ctcgatttcg atcaacgcga gtggatctcg
    19681 cacagcggct ggccgttccc gcagaaggag ttcgacggct attaccggcg tgcccattcc
    19741 tactgtgagt gtggagagta cgattaccgt gtgtcgaccg cgctccccgg tgcgccgcca
    19801 tcgatgctgc cggggttcga ggacggggat gtgaatacgt cgggtatcga acgatggagt
    19861 ccgccgacgc aatttgggaa agtgtatcgg ccggttctca cacgtgcgga caatctgcgc
    19921 gtactgctgc acgccctggc ggttgaattg cagacctcat cggacggtaa gcggatcgat
    19981 tcggtcgatg tggcgacctt cagcggccct cgctttaccg tacgtgcgcg caccacggtg
    20041 ttggccggcg gcggattgga aacgacgcga ttgctcttgg catctcgagg tgtgcatcgg
    20101 gagggaatcg gcaaccattc ggattggttg ggacgcgggt acatgtccca tattcacggc
    20161 gtcatcgcga gtgtcacgct cacggcagga caggatgtca tgttcgggta cgaggccgat
    20221 ccgcagggtg ttttctgcag acgtcgcatc gccttctcgg aagaggccca gcgtcgacac
    20281 cgtctcttga atctgtacat gctgttggat cggcccttgg tgggggaccc gggccacggc
    20341 aatgcggtcc tttccgcagc gtttttgctc aaacgtctgt tgggagggag gcagcaggag
    20401 gagttaggga agggcaagta ctcactctat tggcggcata tcaagaatat cctggcgggc
    20461 tccccccagg cattgtcggt actcccaaag ttcggtcgaa agcggctgct ccagcgtcga
    20521 cggattcctt cgctgctcat gcggtcccgc tcaaacacct attacctcta cttccagagt
    20581 gaacaggtgc ctcattggga aaaccgtgtg actttggatg ctgcgcgaga tgagttcggc
    20641 acgccgcgat tacggcttca tttcgaagtc atggagcagg atcggcagag tgtgcagcgg
    20701 gcgcatgagc tcattgatca ggaattgcgg cggcagaatt gcgggtatgt gacctatttg
    20761 ggaaacgatg tgtcgcaatt gatgcgcgac gtgaaggcgg tgctgggtca tcacatcgga
    20821 accacgcgta tgtcggcaga tccttcgcag ggtgtggtcg acgcgcagtg ccgtgtgcat
    20881 ggggtagcga atctgtttgt cgccagcagt tcggtatttc cgacatccag ccacgccaat
    20941 ccgacgttga caatcgttgc catggcgctg cggctcgccg atcatctcaa ggcattacat
    21001 tccggcgtca cgcgagtttg agacagtatt tctgtgggaa aggggaacat tatgtattgg
    21061 aaaaacaaac gtgtgcttgt caccgggggc gcgggtcaga tcgggtccca tctggttgct
    21121 cggctcgctg ctgcgggcgc acaggtaacc gtggcggaca atctatggcg tggaaagaag
    21181 tcgaatctgc ttggagacga cggagctccg gtcattgaca tggaaagccg gttcttagag
    21241 ctggacctct gcgattatcg gaattgcgag cgggcgacgg agggacaaga tatcgtctat
    21301 cacctggccg acgtggttgc cggcatcaac tatgtgttcg gcaatcagtt ctcgctgttc
    21361 aatacaaatc tgatcatcga ttccaacatg ctccgggcgg ctatcgccaa taaggtgtcg
    21421 tcctacgtgt atgtggggac ggcatgcagt tatccggccg aacgccaaag caaattgaac
    21481 ccgccgcctt tcaaggaaga cgatgtgtat cccgcatcac cggagtcatc ctatgggtgg
    21541 agcaagctga tgggagaaat gggatgtgat ttggccagaa aagagaagct gttggatatc
    21601 ggaatccttc ggtttcacaa cgtctatgga ccccactgcg aaatgtcgcc cgagaaatcg
    21661 caggtcattc catccttgat ccgcaaggcg gtccggcatc cggatgagga attcgtggta
    21721 tggggatctg ggaaacagcg ccgcgcgttt gtgttcgtga gcgatatcgt agacgccttg
    21781 gtgtcagtgg ccaccaaagg cctgaatcag ggggtgattc agatcggacc tgattacagc
    21841 acctcgatta aggagatcgc ggaactggtg tgtgccattt ctggaaagcc gatcgatatc
    21901 cgattcgaca ccagcaagcc cgaaggtgat gtcgaccgag cggccgattg gaccagagcg
    21961 cgcgacatcc tcggctggtc gcccaagacg agcattcagg aagggctgga gcggacctat
    22021 gcgtggtgtg atcgccaggt gtttcaaggc gtagggcggt aggaggcggc cggtttctct
    22081 caggtagatc cagcgaactg aatgtgtggt gacccaggct ggatgggtcc gcgtatctca
    22141 gttgtaacaa cgaagtgagg caacatcgtg aagaaagcat tgatcaccgg catcagcggg
    22201 caagatgggt cgtatctcgc agagttattg ttggcaaagg gttatgaggt ccatgggatt
    22261 atccggcgct ccagctcttt caataccggc cgcatcgatg cgatctatca ggatccacac
    22321 gttgccgaac cgagactacg gctggtctat ggagacctca acgatgctag ttcactcaat
    22381 aaaattctgc gcacgatcca gccggatgag atctacaacc tgggcgcgca gagccacgtg
    22441 cgggtaagtt tcgatgtgcc tgaatatacg gcggaggtaa cggcactggg gacggtccgt
    22501 ctgttggaag ccattcgcga atccggtatc caacccaagt tttatcaggc ctcctccagc
    22561 gagatgttcg gcaaggtgca ggagattccc caacgggaaa gcacaccgtt ttatccccgc
    22621 agcccctatg gcgctgccaa ggtctacgca cactggatca cggtcaacta tcgtgaggcg
    22681 tacaacctat tcgcctgtag cggcattctc ttcaatcatg aatcgccccg gcgaggagaa
    22741 acgtttgtta cccggaaaat tacgcgtgcc gcggcccgaa ttaagctggg ggtgcagcag
    22801 gagctgtatc tcgggaatct cgatgccaag cgcgactggg ggtttgccgg cgattatgtg
    22861 caggcgatgt ggatgatgtt gcaagcggat caaccggatg actacgtgat cgcgaccggc
    22921 gaaacgcata cggtgcggga atttctcgac cgcaccttca gctatctcga tctggattgg
    22981 aagcaatacg tgaagatcga tccgcgttat taccgtccca cggaggtcga tttgttgatc
    23041 ggcgatgcca caaaggcgaa aaaggtctta ggctgggagc cgaaggtatc attcgaagac
    23101 ctgatcacca tgatggtcga gggggatctg cgcgccgaac ggagcatcct cgatggcaca
    23161 agaggagcac gctgatcatg tcgttttgga aagacaagcg tgttgtggtg accggcgggg
    23221 cgggctttct aggcacggtg gtggtcgacc aacttcggaa gctaggctgt cgaaacatcg
    23281 tcgtcccacg gagccatgac tatgatcttg tggacatggg cgacgtcaag cggttgtatg
    23341 tggatgcagc tcctgatctg gtgttgcacc tggctgctcg cgtgggcggc atcggggcca
    23401 atcaaaccca tgccggtcag tttttttacg acaatcttat gatgggaact cagctaatcg
    23461 aggtgggccg tcagcagggg ttaaaaaagt tcgtggcagt aggaactatc tgttcctatc
    23521 caaaatatac gccggttccc tttcgggaag aggatctctg gagtggttat ccggaagaga
    23581 ccaacgctcc ctacggcttg gcgaagaaga tgatgctggt ccaagcgcag gcgtatcggc
    23641 aacagtacgg attcaacgcc gtcgttctgt ttccagtgaa tctctatggt cccggggata
    23701 atttcgacat gcagacctca catgtcattc ctgcgttgat cagaaaatgc gtggaggcaa
    23761 aggagcgtgg tgacgacaga gtcgtcttgt ggggcgacgg ctcaccgtct cgggagtttc
    23821 tgtatgtgac agatgccgct cgtgccattc tgctcgctgc tgagcattac aatggaaatg
    23881 agccggtcaa tctcgggacc gggcaggaaa ttactattca ggcgttggcg catgtgattg
    23941 ccgatgccgt ggggttcacg ggtgaaattg tctgggacac aacgaagccg aacggacagc
    24001 cgcgccgatg cctggaagtg agccgagcca agcaactatt tgggttcgaa gcaagtactg
    24061 cctttgttga cggtattcag cagaccgttg cctggtttta cgccaatcgg ggatccatcc
    24121 gggaagtggt gttttgactg ttccaacatc acttatctag ttcatacaat tcgggtgcag
    24181 ttttccatcc gagaggtaca cgcaagagag aaacgctgac ggggtcgtcg tgacgacatt
    24241 ttttaatgct gtgccttgtt gtgtccctgg acgaattaaa cagtcatagg ggaagacgcc
    24301 gggtatcagc cacgatggat gtgccgacca catagcaaat ccgtggattt gtggatatcc
    24361 tgaaataatc cgtggtattc cggtcgcaat tttctgtgcc gcccagctag agttctgcga
    24421 cattccgtca ggaaagccta attccatgaa aaataccggt ttggttggcg ccactgtctt
    24481 cacttcttgc atgactttgt ctagagctgc gaagtgattc tcgatgggaa tcacgtagtc
    24541 ctgcgtcatt ccaggccagt ccttcgcata gtaatcgatc atgtagaaat ttaagctgat
    24601 ccagtcgatg tattgatcac ctgcgtagaa atttgaaaag gcatgacagc tttctagcag
    24661 ctggcgcgca taggctttgt cgatggtgga gggcagttca tttacgtcat attcgatctg
    24721 attcacttgg ttcactgacc atcccattgt atcaaaggtc agaaatttaa ggccctcccg
    24781 acggacgaag aaatcatagt aatagcgttg cgcggctttc agacgttcag ttccatcgca
    24841 cacttgggga gttccaatgt cactgtagag tgacgcatac ggaagcgaag acggattgaa
    24901 ctcagaaaac ccgcgcttgt tctcgatggc ccagcgcagc gattgatcac cagctggtcc
    24961 aaagccgcca aaatagtctg ctccaattcc gttcggctct cgccctgcga taaaaaatac
    25021 aggttttccg tattgcctta gttctgcggc aaatcttcga agttccgcgt catagactcc
    25081 gttcagcaac ttatcgaccg tgaatccctg aggggcttca gactcaccgg gcgcagggtg
    25141 tgtgttgtag gcctggacca cgatcacttt tccttcctgc catgccagat tggcagcctg
    25201 tacgtccaga tggggttgac ctgccgcgtc gtatccgtta ccccacgagc cacgatattt
    25261 tgaccacagc gcagtctttc ttccgctggc cgattcaaac gatgcaatat cattctggac
    25321 ccattcgtat tggcccatgt agaggccgcc ggctggcgga ggaacgatct tcagtatcga
    25381 atagtcacgt gcagcggtgg tttgtgtcgt cgggggtgtg acggattgcg tttgtacctg
    25441 tcgttttcgg gagaaccgag attgtgcttc tgctgtagag gcgtcgaatg agatccagat
    25501 tcctgcaatg aagctcaggg caaggactgc tgcatagtgt attttcacgg tgagacctcc
    25561 gagtatctgg tccttctagt gagcaagggt cgtaccatgg gaagggaaat ggggaagccc
    25621 aaaaatcaag ggaaatggca atttactcag gatatgcgct ctgtggcgag gcacattctt
    25681 gaacagaatg ctggtgggac cattcaggtc acatacacag aaggatactt gttcgtatcc
    25741 agcaggtagg ttctctacgg tttctccatc cccggaatac attcagtcgg cataagttcg
    25801 ccaccgtgaa aatgtgagac ttgtttccat ttctgcagca cggctgcacc atcgaatgtc
    25861 agcagatatt cttcacacca gatgccgggc gcggtgtacc gattgccggc ctgttgttcg
    25921 cggatttcat agacccacac cgtctctccc tcgtcgctct tccgtactgc tttaggcgcc
    25981 cctagttcct gcctcacctc agtctgtgtt gcccgccctt tgactgaatc cagatacgcc
    26041 gcttctctgt tttgaaaatg actgcaggcg gtgatcccga tgagtatggc catcaggccg
    26101 aaggtccaag agcgtgtcat catctacctc ccgattcgag ggtgatccgg tttggagatc
    26161 tgctgtccca ggcgggattg tacaccgatc ctatggattc gtctgcttca tctctcctcg
    26221 cagatgggcg ggggatgaac ggcgaagagg cgcagaatgt cgcgtactgt agcctcgcga
    26281 ggggatagag ttgtgcgggg gtgataaggg aacgagcggc gcgagagggc gcacctcgag
    26341 tgtgaaatca aatcatgggc tgaacatgga agggaagcgc gggagagatc agaccgcttt
    26401 tttaaccaga cccgaccgca ggca
//
LOCUS       DAOQEW010000030        26265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_431_length_26265_cov_8.351164, whole genome shotgun sequence.
ACCESSION   DAOQEW010000030 DAOQEW010000000
VERSION     DAOQEW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 26265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26265
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_26265_cov_8.351164"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(568..1989)
                     /locus_tag="PLS35_05280"
     CDS             complement(568..1989)
                     /locus_tag="PLS35_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18100.1"
                     /translation="MSGAKSKTFTILLVEDNAGDVDLFLRTVEHELPRHEDDEVTLVL
                     NATAEGALERLRQQPIDLVIADVRLPGMSGIELLRQVQDLNRRIPVIILSRVDATETV
                     IDAMRHGAFDYIVEPYDCMDLAARIHRAMRMSEILLQGSPDEPAAPRIPFKNLVGVSP
                     AIRNVMAMIEAIARVPSTTLIIGETGTGKELIAKAIHERSLDSDGPFQVVDCTTFSEG
                     TVESELFGHVRGAFTGAVADRTGLIESGSHGTVFLDEIGDLPATLQAKLLRVLEAGEV
                     RAVGSTQQRKVNVRFVAATNQDLAEKVKRNEFRKDLFFRLNVMVIRVPPLRERTEDIP
                     VLARHFIARYAKEFTKRVDDLHPAAVTELVAYPWPGNVRELRNVMERAVMLAKGDRIG
                     IADVAAMLAVPDERQRDTPDEDYLHLPYAKAKEQVLEAFNRRYISTKLTIHQGNVTHA
                     AQDAGLPRSYFHEIMRRYSKDEK"
     gene            complement(2171..3358)
                     /locus_tag="PLS35_05285"
     CDS             complement(2171..3358)
                     /locus_tag="PLS35_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:proton antiporter"
                     /protein_id="HNI18101.1"
                     /translation="MLPVATPEPQESTLLRFPNRNTPPRYDRAIGEWALVIPLGTAGL
                     FFVHGHEWLHDLSNPLRLSAMLAWLLLVIAFSAFAVLRHAEHLAARLGEPMGTVILTL
                     SVTGIEVMMIAAFMYTGNGNASLARDAMFAVVMIVLNGMVGLSLLLGGLRYHEQTYNL
                     QGANAFLAVIVPLAVLGLVMPSYTISSPGPTFSPHQAIFLIVMSLGLYGVFLAIQNLR
                     HRNYFVAPRRQSRRNAAILHEHQPSPGGSVWFHTLLLLAYLLPLVILSEHVATPIDYT
                     LRLWNAPHALGGVLVAGLVLAPESLGAVRAALANQLQRSVNILLGSVLASISLTIPAV
                     LAIGFFTDSTILLGIDPVDTILLVLTFVVSMLTFAVRRTNVLLGAVHLLLFLAYLMLI
                     FEK"
     gene            complement(3400..3714)
                     /gene="sugE"
                     /locus_tag="PLS35_05290"
     CDS             complement(3400..3714)
                     /gene="sugE"
                     /locus_tag="PLS35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HNI18102.1"
                     /translation="MAWTFLIVAGLFEICWAIGLKYTDGFSKFWPTAGTLMAMAASFG
                     FLAQALKSIPVGTGYAVWTGIGAAGTAVLGIVLFAESVSIVKVFSLLCIVLGIIGLKG
                     SA"
     gene            complement(3846..>5699)
                     /locus_tag="PLS35_05295"
     CDS             complement(3846..>5699)
                     /locus_tag="PLS35_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI18103.1"
                     /translation="IYRHLSEHRDSREGNVSTILRAAMEVGQPVVFGILIIIIVFFPI
                     LSLQGMEGKMFQPLAYTIIIALLISLLLSLTLSPVLCSLVLKDAREEDTLLLRWIKRA
                     YMPALGWALRHRTVVLGSALVMLAASLALFPFLGGEFIPILNEGSVSPQTIRLPGISL
                     EKSIEIEKEMQRAILEFPEARMVVSKIGRTELGNDPQEPNSSDPVVSLVPMDQWKTAT
                     TKAQLDDAIRRRLQRIPGANFLISQPIQQRVDELVSGVRSEATVKVLGDDLDVLRATA
                     DKIQTIMTGIDGVKDVRVEQLFGQPYLTIDIDRGQIARHGINVAHVREIITTAIGAEP
                     ATRVYEGQRRFDLILRYPEQYRDSVDTVSNILLTTPTGALIPLGNIAKVELREGPALI
                     SREGLQRRIYVGFNTFGRDIESIVHEAQTKIARQVPLPTGYRLQWGGSFENMQRAMAR
                     LKIVVPLTIGVIFLILFSSFNSLRHAALIILNLPFALIGGIAALWLTGEYLSVPASVG
                     FINLFGVAVLNGIVLVSYINKLRAEGMAGEEAVVTGCSLRLRPVLMTAVVALLGLLPL
                     ALSHGIGSEVQRPLAIVVIGGLVSSTLLTLIVLPVLYQWFDRPDKSAAPAN"
     assembly_gap    5700..5799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5800..6824)
                     /locus_tag="PLS35_05300"
     CDS             complement(<5800..6824)
                     /locus_tag="PLS35_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI18104.1"
                     /translation="MVSSLLQLALRQRVLVVVIAFALALGGLYAFQTIPIDAFPDVTT
                     VLVQVVTKAPGLSPEEIERFVTFPLELQLTGAPGLVEVRSLSKVGLSMIDVIFEDQVD
                     IYLARQVVLERVLEVQDQLPPGSISQLVPNTTGLGEVFQYVLKGPQDDAAGGLVTEQE
                     LMMRRTLQDWLIRPLLKGLPDVVDVNSLGGFVKQYQVMVDPGLLRKYNLGLHDVFAAV
                     QQNNANAGGNILEKNSERYIVRGVGLIKTVADIEQIVVKEVGGTPVYVRDVAEVRIGS
                     AIRHGAAVINGQQETVTGIVLMLRGGNAREVVQAVKTKIDDIHAHKLLPDGLRIVPFY
                     DRIELITA"
     gene            complement(6829..8058)
                     /locus_tag="PLS35_05305"
     CDS             complement(6829..8058)
                     /locus_tag="PLS35_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18105.1"
                     /translation="MAAACHDHHAEVSPRHARRWTARGISLLFVVLLASSGCDGPPPN
                     ATGANPPVAPAESGSIRLTAEKVKSAGITVQPLRRGEFRTFRDFPGTVEPDEHALAEI
                     TTLVRGRVIDVYADLGREVKGGTLLALLYSSELGMAQSAYLKATAKLNVADRAFRRAE
                     LLLKEKVIGLAESQRREGEMISLRAEQREARDRLLLLGLTDEDLRHLDKNQTIRSHVP
                     VVAPFDGRIIARNLTKGEVVETTEKLFVLADLSEVWVTAKIPEKDIPYIRTDQTGTGQ
                     LVEVHVSAYPGQIFQGSITYVGDVLDPATRTMRLRLELPNPQRKLKPEMYATVRVYSD
                     PEPNVLLIPETAIQRDRDRQFVFVEREPGLFEIRPVQLGDSNGKEVKVLGGVEDQEAV
                     VVTGSYVLKSELLGSQI"
     gene            complement(8058..9434)
                     /locus_tag="PLS35_05310"
     CDS             complement(8058..9434)
                     /locus_tag="PLS35_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI18106.1"
                     /translation="MNRRSPMQWGNDQARFTRGSVNVWHLRLTYHMTIGCLVAMLSGM
                     AWSSPCLSWAASPSTMSSSASEPVYSMATVLDLALARNPTVASAEGTIEQNRGQQVAA
                     HTYLNPSVNANSGRGRMRDLGLFDPAVRESVTEFNLSVGQPIEWPSKRAARQRATEAG
                     VASASAGLAETRLNLIADVKVAFYDLLLAQRDLTLAQQNLATIEDVHRAVTTRVRLGE
                     SPQFEAIRSEVEVLKAKQSLTSANNRMRVGRVMLDTLTAGSLGPSYAIDGQLHRVGPG
                     FGIDILTERALTEHPTIMRLRKAVEQADHSLEFERQARVPNITIGGSYWREIGREAFT
                     GGVSFPTPVWDRRQGEIISAFGSKRKGEAEYLRARNELIRNISQHFQDAKTTADLIEV
                     YEKGLLRQAEEALRIAKFSFQQGASSLIEVLDAQRVQRQILLDYAQAQFDLSISLALL
                     ERAVGGNL"
     gene            complement(10056..10256)
                     /locus_tag="PLS35_05315"
     CDS             complement(10056..10256)
                     /locus_tag="PLS35_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18107.1"
                     /translation="MSPTATRCQFCWRVEDNRTGGTDSAAWSDLPTFMQKHNLVSHDL
                     NFADGYCPQCTQLYGRLTQEHP"
     gene            complement(10441..11085)
                     /locus_tag="PLS35_05320"
     CDS             complement(10441..11085)
                     /locus_tag="PLS35_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18108.1"
                     /translation="MTTPPRVLLADDHTLVLEGFRRIVEQRCEVVGAVEDGRALLEAA
                     MQLRPDLILLDISMPLLNGVDAGRQLKKLLPDVKLIFVTMHADPAYVSEAFKAGASAY
                     LLKRSAARELDQAIDAVLKGQYFVTALLTRDIVTGLSAGQSGLFAQRQELTPRQREVL
                     QLIAEGRTIKEIAALLNISPKTVEFHKAQIIFHLNLRTTAELTKYALAHGLTSA"
     gene            complement(11082..>11906)
                     /locus_tag="PLS35_05325"
     CDS             complement(11082..>11906)
                     /locus_tag="PLS35_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNI18109.1"
                     /translation="EIELREAKDELEVRVDARTKELADVNRSLLQEISGHIQTEGFLR
                     NSEQALATSRQELRDLAARLLTVQEEERRRISRDLHDDINQRLAMLVVQAESLEANLP
                     ASAVGCSKELRSIQDRLTELSDDVRHLAYQFHPSILDDLGLPVALQRLVDECAVRSNL
                     AISFETDASPKAIPQTVSTCLYRIAQECLSNIMKHAHASQAKVSLASTAEGITLTVQD
                     DGVGFDTQQVVDNPRGLGLVSMAERVRLVHGTVTIDSIPQQGTRLSIHVPRAEVSV"
     assembly_gap    11907..12006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12007..13316)
                     /locus_tag="PLS35_05330"
     CDS             complement(<12007..13316)
                     /locus_tag="PLS35_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18110.1"
                     /translation="MQATIYVTDDEPAIRSAIVKRLSRRHHTVTGFESGEDLVSAVAQ
                     QAPDLILLDLKMPGMGGIEALRKIRPLAPQTLIIMLTAYGTVENAVEAMRLGAYDFLI
                     KSVDLSGVDPVVDRALEFLALRHRVQFALEDQNSAYALANIDARSPAMQLLLGQVRDV
                     AENAKSTVLLQGETGSGKEFLARVIHCNGPRAAGPFVAVNCTAIPKELFESELFGYER
                     GAFTGAHQRKRGLLEKAEGGTLFLDEIGDLDPAMQAKLLRVLQERTFRRLGGTEDLSV
                     DFRLMTATNRDLKKDTARGTFREDLFFRLNVVTFELPPLRVRAEDILPLCMQAMLRFG
                     KEFGKEVIDIEPDAKELLQRYSYPGNIRELHNIIERAMILCHDKTLTAGCLPRELRDQ
                     ASTHVAVAMAQGEHPSLRIEMVLGQQTLADIESALIEEVVRLSD"
     gene            complement(13288..15243)
                     /locus_tag="PLS35_05335"
     CDS             complement(13288..15243)
                     /locus_tag="PLS35_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18111.1"
                     /translation="MSDLLSNLPIARKLFLASVIPALTVLLLSILTYRSVTTFSDDEG
                     QLNDIYYSQRLASEYLRLVVDLETGFRGFVLTSQEHYLFPYRTAQDHVLNIGRTLEAQ
                     VSQQDDQRALITSVQQLVKQFISEKEVLIDAVKAEHPNEAREYIEEGKGRALMLKIRQ
                     DMGRFEHLTQTALNTKLAKIAQDRDTMLMTILGGGLFSLICMVAALHLIAHSITTPLA
                     RLATAVVTSDRNPIPQVPVMTRTDEIGNLARVMHAMSTAIHSHIEALQQSEGNLRQLN
                     QDLAGSEAKYRSIVDHAPFGIFTTKGMTLVFSNRYNSLLAGLNSTEEKDPDAVRRAIH
                     PEDRDRVIAEFAQAVVEGEPYETVFRFLHADGTIRKVLSRRIPIRDQSGQVVMYQGFN
                     VDITALDFMQTRLRRAERLATLGQVAAGIAHEIRNPLVGIGSTTSLLLDDTDASDPRR
                     PDLAVILQETKRLDRIVNQIIDYARPREIVAFTFDMAQLVHEVMKVLDEPLTRKQAVI
                     NLSAPAAPYMIQADRDQFKQVLLNILQNAIEATPQGETITVALAEQARGIESGLEITV
                     TDQGSGISPSHLPHVFEPFFTSGKPRGTGLGLAISRNILDAHGGDIALDSEEGRGTTV
                     RVWTPVRQQPQRMQEEGDHAGHDLRYG"
     gene            15661..16533
                     /locus_tag="PLS35_05340"
     CDS             15661..16533
                     /locus_tag="PLS35_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNI18112.1"
                     /translation="MDYVKPYGVVDSMLAGGALKLSLPPRQLVLRGMLAGAYLGIGTS
                     MAVTVAVETGYWILGSLVFPFGLALAILLGAEIITGSFALLPVAAAHGQKNAGLSRVF
                     ANWGWVFLGNLLGSVLYATLFAIALTTGGDAPINAVGNKLIAIAQAKTTYYASHGSAG
                     LLAAFTKGMLCNWMVSLAVVAAYMSTSLSGKFVAIWGPTLLFFSQGFEHAVVNMFLMP
                     VGMMLGADVSLSTWWLWNQIPVTLGNLVGGMLFTGLAIYAAHHATAPAAAAAQAPAQP
                     VTARGEQAGYSPSY"
     gene            16552..18150
                     /locus_tag="PLS35_05345"
     CDS             16552..18150
                     /locus_tag="PLS35_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNI18113.1"
                     /translation="MAAVYLRRLHGWRFVLFNAVLGLSHIVVLFNAGSYIALLPHVSG
                     DLGGVLPSFLTWAQTDFMVGLALGFPLARYLSGRIGDYRLLIGAFMVYSVASYLCGDS
                     RTLAQFVPARIVQGIAGGITLPIAQSMLLNEYPKRLKAVALTVWGLFSITPFTIGMPV
                     GGYIAYMLGWRFLFYLDFVLTLVIVGTLGALLYGRGFRRRYARFDLVGFALLAILLLG
                     LQTLLNMGNDFDWLDSPFLQVIAVVLLIAFPCFIIWELGERHPTLDLRLFLHRNFVIG
                     IISLTLGFFSIQGLLSLLIVQIQLILGYSSKLAGLALLPLVLLSAPIIAVMHYLCKYV
                     DARWLVCLNCLGFAWTFYWIGLYDDPHSYEELFWPMVVEGIFLGSFFTPITVLTLHGL
                     SGPLVMRAAEVANLLRVAAGAFGITFQGIVLFRRIHFHQLHLADHFGGRQSISYDPMG
                     QLAAKFQAAGLSVSEAQAKLGLVIKQEAAILGLNDAFLVASFIFVGLGGLVWFAHPTH
                     VPIASSPEDDLRVRRAEELIEEVP"
     gene            18147..19655
                     /locus_tag="PLS35_05350"
     CDS             18147..19655
                     /locus_tag="PLS35_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNI18114.1"
                     /translation="MRSGSTVGQRVWRCLGIGCVLLFGSCAWIPKGDPPAQYLDPPEM
                     TETLAEITSRLQNWPDDHWWEQFGNPELNGLIETALKDNPGLKHASARLRQATSLVKV
                     EGARLLPFLEADASLTYERISQHGVFAALNPEVAGIKIMYGIINPLSFRYEFDFWGKN
                     RAMLEGALGHAAAEEAETAEVRLRLTTGIARAYFRGQALQQQLTIVKTIVGIRRDLKN
                     LAETRFRLGLDNDQPFKIAVADYEAAFKRQAAIRDQLDVQRHLLARLAGKGPDEAAHL
                     FAKPMANIPKQIPAPDHLSIGLLVHRPDLAAALYRAHAASRLVRVATTQFYPTIDLTG
                     FVGFNALTLAKGTDKLANFLFSGQSFTYGLAPGLRMPWFEGGRLRGELGAQRAEYDAA
                     VELYNDTLLDAMREVADSLSAWHTTNEIMASHKRLVASLGEDWRLAKVRLVNGLDDDR
                     EVLRHRYPVLEQEYALRALESDQLVAAVDLIESLGGGYHNPDIEKRPKHNPS"
     gene            19660..21207
                     /locus_tag="PLS35_05355"
     CDS             19660..21207
                     /locus_tag="PLS35_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNI18115.1"
                     /translation="MTTTTADMSQPPQSPGVPPPAGSYRIHPKAIRARRNRRLLVVAL
                     LVLIATVGYLMYWYTHDRFWIRTDNAYVTGNLVPVAAQASGIITQVLAEETQFVNRGD
                     LLVRLDEHQAYAALGRARGRLGEEVRRIAALFINRKQLAEKLRSRMARLELAEHDMDR
                     YQRASPSGAISKQILQNTADKIASLEAEVRETQAELDSLDAQIGGTTVMAHPSVDLAK
                     HQLIEAHLEYARQQIRAPISGYVAKRKAQVGDRVQPGASLMTIVPLDHLWVEANLRET
                     ELQHVRPGQPALVSVSLYGSKQTFHGTVEGLVPGSGSAFALLPPDNSTGNFIHIVERV
                     PVRIALPADELREHPIRPGLSTMTSINITESGQSVWTSLATPSTAEYETDVYADELPT
                     AESMAKEVMATNLVVSENGDGIDSLLEDEGAIERVNPRRDVDRLPNEGKAAPKLDRLR
                     ERDDRRPSSFVPRRSPDLGATTFPLTPSIGPDSRSLGPEAGRNPSRLKPGFGLDDERR
                     AFGSGRR"
     gene            21474..22994
                     /locus_tag="PLS35_05360"
     CDS             21474..22994
                     /locus_tag="PLS35_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI18116.1"
                     /translation="MPNVTLNYRTLLSVTNVLNSQRNRQSLFRAITDQLTKVVRWERA
                     GITVYDLQTDAFRFYAVETNLPKVVLRSDAMIPRAQSAVGWVYDHQRVHVRPHLREER
                     LFIEDESYAQEGLGRMINLPMVVQEACLGTLNIGSIEAGYPDPDDVDFLQQVATQIGF
                     AIAHVNAYEEINRLREQLARENVYLTEELKATQDYGIVAGRSRAFEAVLTLARQAAPT
                     TATVMITGETGTGKEVLARAIHERSLRRERAFVRLNCAALPSGLIESELFGHERGAFT
                     GADHQRVGRFELADGGTLFLDEIGEMPLEAQAKLLRVLQDGMVDRVGSAKPIPVDVRV
                     IAATNANLERAIQQGRFRSDLYYRLNVFPIHMPPLRERPEDIPILARHFLRFHARRLK
                     RLCEDFDASSMERLVQYAWPGNVRELENLVERGLILCHDRWLRIDPAMINLPAASEVN
                     PRRTLVDEERRHILQSLTLLDWRIEGPGGAAEQLGLAPSTLRSRLQKLGIRRPSRC"
     gene            23454..>26265
                     /locus_tag="PLS35_05365"
     CDS             23454..>26265
                     /locus_tag="PLS35_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNI18117.1"
                     /translation="MMQLSRRQFLKASAGTVAVAAVADKALALTALQPVVEVDNPLGE
                     YPDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDL
                     YGNRGTFAHNPRMCLKGFTFHRRVYGPYRLKGPLMRKGWKQWMDDGSPELTSDAKRKY
                     KFDSRFLDDMVRVSWDTAFTYVAKGLVVIGTRYSGEAGARRLREQGYAPEMIEMMKGA
                     GVRTFKHRAGMPILGMMGKHANTRFNNCVLPLLDSWIRKVNPDQAQGGRYWNNYTWHG
                     DQDPSQPWWNGTQNCDVDLSDMRFTKLNTSWGKNFVENKMPEAHWKLESIERGARLVV
                     ITPEYNPTASRADYWIPVRPETDGTLFLGASKIILDENYQDIDFIKGFTDMPLLVRTD
                     TLQYLDPFEVVKDYQLPDFSKSYSGRLQGLTQDQIRRLGGMMVWDLAKGKAVPLHREQ
                     VGVHLAQSGIDPALTGTYRVKLLNGREIDAIPIYQMYQIHLQDYDLDTVHQVNRAPKD
                     LIVRWARDCGTVKPAAIHNGEGVCHYFHMTSMGRAAALVMMLTGNIGKFGTGCHTWSG
                     NYKVGIWQAAPWSGAGASVYLGEDPWNLNLKDDVHGKEIKYRKYYYGEEPGYWNHGDN
                     ALIINTPKYGRKVFTGKTHMPSPSKVRWVVNVNILNNAKHHYDMVKNVDPNIEMLVTQ
                     DIEMTSDVNHADVAFAVNSWMEFTYPEMTATVSNPWVQIWKGGIRPLYDTRNDLDSFS
                     GVAAKLKEMTGEQRMADTYKFVYHNRVDVYVQRILDASSTFFGYSADVMLKSEKGWMV
                     MCRTYPRHPLWEETNESKPHWTRSGRLETYRVEPEAIEYGENFISHREGPECTPYLPN
                     AIMTTNPYVRPDDYGIPVTAQHHDDKTVRNIKLPWSEIKQHANPLWEKGYQFYCVTPK
                     TRHRVHSQWSVNDWVQIYESNFG"
BASE COUNT         5252 a         7364 c         7905 g         5544 t
ORIGIN      
        1 ggactttgtc cgccacggcc acggccgcga cggtgcccgc cgagattttg agaaactgtc
       61 tccgtgaaac ggaaacctgc ataatagcta cctccttgtg agatgcatga gtccctcatg
      121 cacctgttcg tacaacttca ctacactcac gtggatgcaa tctcgtctgg tgtaccccct
      181 ttccgcgtgt cgaattggaa acattcgtcc ctcacaattc gaaaatttcg agcggacatt
      241 cgctcgcggt ataggagtat tacaattcac atgccgaaag gggagaaatc tggcgctctt
      301 tggatggcaa tttagtgacg attaatgcag taaaatcaat tgattgcgtg atggtgtttt
      361 cgagggagtt ggatggggaa ggaaggcctg ataggcaggg tcaggaacta ctgactaggg
      421 agaaagttca gaggcttaag ttgttgatat taaagatgaa ggcagtgtct gtaaatgcgg
      481 acggcgtcat gagatgcatg aaacgtgatt taaaacaata gcttgtgcat ctatgtcggc
      541 ttttggtgtc atgctttact gacaccatta tttttcgtcc ttggaatacc ggcgcatgat
      601 ctcatgaaag tatgaacgag gtagtccagc atcttgggcg gcatgcgtga cattgccctg
      661 atggatggtg agcttcgtgg agatatatcg acggttgaac gcttcgagca cctgttcttt
      721 cgcctttgcg tagggcaaat ggagataatc ttcgtcggga gtatctcgtt gccgttcgtc
      781 gggaacggcg agcatcgcgg ccacatctgc aataccgata cgatccccct tggcgagcat
      841 gaccgcgcgc tccatcacgt tgcggagttc acgcacattt cccggccagg gataggccac
      901 cagttccgtc acggccgcgg gatggagatc gtccacccgt ttcgtaaact ccttggcata
      961 acgggcgata aagtgtcggg caagaacagg aatgtcttcc gtccgttccc gtagaggcgg
     1021 cacgcggatg accatgacgt tgaggcggaa aaacaggtct ttgcggaact cattccgctt
     1081 caccttttcg gccaggtcct gattcgtcgc ggcaacgaag cggacattca ccttccgctg
     1141 ttgggtgctg ccgacggcac gaacttcacc agcctccagg actcgcaaga gcttggcttg
     1201 gagagtggcg gggagatcgc cgatttcatc caggaagacg gtgccgtggc ttccggattc
     1261 gatcaagcct gttcggtcgg ccacggcccc ggtaaatgcg cccctgacgt gtccgaagag
     1321 ttcactctcc actgtgcctt ccgagaaggt tgtgcaatcg acgacttgaa acggcccatc
     1381 actatcgagg cttcgctcat gaatcgcttt cgcaatcagt tccttgcccg tgccggtttc
     1441 tccaataatg agcgtggttg acggaacccg cgcgatggct tcaatcatcg ccatcacgtt
     1501 tctgatcgcg gggctcacgc ccacaaggtt tttgaacggg atacgagggg cagccggttc
     1561 gtcgggcgag ccctggagaa gaatttccga catgcgcatg gctcggtgga tacgggccgc
     1621 cagatccatg cagtcgtatg gttcaacgat atagtcgaac gcgccgtgac gcatcgcatc
     1681 aatgacggtc tccgtggcgt cgacgcggct gagaatgatg accgggatac gccgattcaa
     1741 atcctgcact tgccgaagga gttcgatacc gctcatgccc ggcaagcgca catcggcgat
     1801 cacgagatcg atgggctgct gccgcagacg ttcgagcgca ccttcggctg tggcgttgag
     1861 gacgagcgtc acttcatcgt cttcatggcg gggcagttcg tgctcgaccg tgcgtaagaa
     1921 tagatcgacg tctcccgcgt tgtcttcgac gagcaggatc gtgaacgttt ttgatttggc
     1981 gcctgacatg cgtgtctttt cagaaaacac tgacaatccg ccgaggaact cggaatgtga
     2041 aacctgtcca gcgtggatct gattggcctt caattcccgg cgtaactggc cgcctctcgc
     2101 ccgtatctta ggaggcgaag gcgcggcaaa tcaacccggc ggcttattcg gagggagacc
     2161 gatttttcgg ttatttctca aaaatcagca tgagataagc caggaacagg agcaggtgaa
     2221 cggcgccaag gagaacattc gtgcgtctga cggcaaaggt gagcatgctt accacaaacg
     2281 tgaggaccag gaggatggta tcaaccggat cgataccgag caggatcgtc gagtcggtaa
     2341 agaagccgat ggccagcact gccggaatgg tgaggctgat gctggcgagc actgatccca
     2401 gaaggatatt gaccgaacgt tgcagctggt tggccagcgc ggcccgcacg gcaccgagcg
     2461 attccggcgc cagcaccaaa ccggcaacca ggacacctcc caaggcatga ggagcattcc
     2521 aaagtcgtag cgtgtagtcg atgggcgttg ccacatgttc tgagaggatt actagcggca
     2581 gcaggtatgc cagaagcagc agtgtatgaa accacacgga gcctcccggg gaaggctggt
     2641 gttcatgcag gatcgccgcg ttgcgtctgc tctgtctgcg cggcgccacg aaatagttac
     2701 gatgacggag gttttggatc gcgagaaata cgccatagag tccgagcgac atcacgatga
     2761 gaaagatggc ttgatgtggt gaaaaggtcg ggcccgggga ggaaatcgta taactcggca
     2821 tgacgagccc cagcaccgcg agcgggacga tgacggcgag aaaggcattg gctccctgca
     2881 aattgtacgt ctgttcgtga tagcgcaatc cgccgagaag gagtgacagg ccgaccattc
     2941 cgttcagcac gatcatcacc acggcaaaca tcgcatctct cgccaacgag gcgttgccat
     3001 tacccgtata cataaaggcg gcgatcatca tgacctcgat cccggtcacg gacaaggtca
     3061 ggatgacggt ccccatcggt tctccaaggc gggcggcaag atgttctgca tgacgaagca
     3121 ccgcaaaggc ggagaaggcg atcacaagca gcagccaggc gagcatggcg ctcaatcgaa
     3181 gcgggttcga gagatcatgc agccactcat gaccatgaac gaaaaagagt ccggcagttc
     3241 cgaggggaat gaccaaagcc cactcgccga tcgcccggtc ataccgaggc ggcgtattcc
     3301 ggttcggaaa ccgcaacagc gtcgactcct gcggttcggg ggtggcgacg ggtaacatgg
     3361 acctgtagac ctcagtgaga gtgggcgcca cctctgtggt caggcgctgc ctttgagccc
     3421 gatgatcccg agtacgatac aaaggagaga aaacactttc acgatggaga cggactctgc
     3481 gaagagtaca atccctaaca cggcggtacc ggccgctccg atgccggtcc agactgcgta
     3541 ccctgttccc accggaatac tttttaaggc ctgagccagg aagccgaaac tggcggccat
     3601 agccatcaac gtccccgccg ttggccagaa tttcgaaaac ccatccgtat atttcagccc
     3661 gatggcccag cagatttcaa acagtccggc cacaatcaga aacgtccatg ccatagaatc
     3721 cacatgctcc ctgcgccatc gcgagatggg aaaagttcag actacgtatg atacaggacc
     3781 atccgcagta agaaacctcg gcatttcact agggacatag agcaaatgcg cgaagagaac
     3841 gacgtctaat ttgcaggggc ggccgatttg tccggccgat cgaaccattg gtagagcaca
     3901 gggaggacga tcaaggtgag gagggtggaa ctgacgagtc ctccgatcac gacgatagcc
     3961 aagggacgtt gtacctccga cccgatcccg tgagacagtg caagcggcag caatcccaac
     4021 aatgccacca cggccgtcat caagaccggg cgcaggcgca gcgagcaacc ggtgaccacg
     4081 gcttcctcac cggccatccc ttcagcgcgg agtttattga tgtacgagac aagcacgatg
     4141 ccgttcaaga cggccacgcc gaagagattg atgaacccga cggaggccgg cacactgagg
     4201 tactcgccgg tcagccacag cgcggcgatt ccgccgatga gggcaaatgg aaggttcagg
     4261 ataatcaacg ccgcatggcg cagcgaattg aacgaggaga acagaatcag gaagatgacg
     4321 ccgatcgtca gcggcaccac gatcttcaac cgcgccatgg cccgttgcat gttctcgaag
     4381 gacccgcccc attgcagacg atagccggtg ggcagcggaa cctgtctcgc aatcttcgtc
     4441 tgtgcttcat gtacgatact ttcaatgtcg cgaccgaagg tgttgaagcc gacgtagatt
     4501 ctccgttgca ggccttcccg gctgatcagc gccggcccct cgcgtaactc caccttggcg
     4561 atgtttccca gcggaatgag cgccccggta ggggtggtca gaagaatgtt gctcaccgtg
     4621 tccacgctat cgcgatattg ctcgggatag cgcaggatga ggtcgaagcg ccgctgccct
     4681 tcatagacgc gcgtcgcggg ctctgcgccg atcgccgtcg tgatgatttc gcgcacgtgg
     4741 gcgacgttga tcccatgtcg ggcgatttgg ccgcggtcga tgtcgatggt gaggtagggt
     4801 tgcccgaata actgttccac ccgcacgtcc tttacgccgt ctatgccggt catgatcgtt
     4861 tggatcttat cggctgtggc gcgcaagacg tccaggtcgt cgccaagcac cttcacggtc
     4921 gcctccgacc ggactccgga aaccaattca tcgactcgtt gctggatcgg ctggctgatc
     4981 aggaaattcg ccccgggaat acgttgcagc cgacgccgga tggcgtcatc gagttgcgcc
     5041 ttggtggtgg cggttttcca ttgatccata ggcacgaggg acacgaccgg atcgctggag
     5101 ttgggttctt gagggtcgtt gcccagttca gtgcgtccga ttttggagac caccatcctg
     5161 gcctccggga attccaggat cgctcgctgc atctccttct cgatctcgat ggatttttcc
     5221 agcgagatac cgggaagacg aatcgtctgc ggtgagacgg acccttcatt gaggatcggg
     5281 atgaactcgc cgcccagaaa aggaaacagg gccagactgg cggccagcat caccagagcc
     5341 gagccgagga ccaccgtgcg atgacggaga gcccagccga gcgcaggcat atatgcgcgt
     5401 ttaatccaac gcaacagcag cgtgtcttcc tccctggcgt ctttgagcac cagcgaacag
     5461 agcaccggtg agagggtaag cgaaaggagc agagagatca ggagcgcgat gatgatggtg
     5521 taggccaatg gttgaaacat tttcccctcc atgccctgga gcgacaggat gggaaaaaag
     5581 acaatgatga tgatcaggat gccgaacacg accggctgcc ccacttccat cgccgcccgc
     5641 aggatcgtcg acacatttcc ttcgcgcgag tcacggtgtt ccgacaaatg ccgataaatn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cggtgattaa ttcaatccgg
     5821 tcgtaaaacg gcacgatgcg cagtccgtca ggaagcagct tgtgcgcatg gatgtcgtcg
     5881 atttttgttt tgacggcttg cacgacttcc cgtgcgtttc cgccgcgaag catgagcacg
     5941 attcccgtga cggtttcctg ttggccgttg atcacggccg cgccgtggcg aatggcgctg
     6001 ccgatgcgca cttccgcgac gtcccggaca taaaccggcg tgccgccgac ttccttcacg
     6061 acgatctgct cgatgtccgc cacggtcttg atgagcccga cacctcgcac gatatagcgc
     6121 tcggagtttt tctcgagaat gttcccgcct gcgttggcat tgttttgttg aactgccgcg
     6181 aagacgtcat gcaatccaag gttgtatttc cgcagcaggc cagggtccac catgacctgg
     6241 tactgtttga caaagccccc cagcgagttg acgtccacga cgtccggcag ccctttcagg
     6301 agaggccgga tcagccagtc ctgcagtgtc cggcgcatca tgagttcctg ctccgtgacg
     6361 aggccgcctg ccgcatcatc ctgcgggcct ttcaggacat actggaagac ctcgccgagg
     6421 ccggtggtat tgggcactaa ttgagagatg gagccgggcg gcagctggtc ctggacctcc
     6481 agcacccgtt ccagcacgac ctggcgtgcc aggtaaatat cgacctggtc ctcgaagatg
     6541 acatcgatca tcgagaggcc gaccttggag agcgagcgca cctcgacgag gcccggggct
     6601 ccggtcagtt ggagttcaag ggggaaggtg acgaaccgtt cgatctcttc cggggacagt
     6661 cccggcgctt tggtcacgac ctggaccagc acggtggtga cgtcggggaa ggcatcgatc
     6721 ggaatcgtct ggaaggcata gagcccgccg agggcgagtg cgaaggcgat gacgacgacc
     6781 agaacacgtt ggcggagggc cagctgcagc agagaggata ccatgtcgtc agatctgcga
     6841 tcccaacaac tcggatttga gcacgtagga tcctgtgacg accaccgcct cctggtcctc
     6901 gacgccgccg agcaccttga cctctttccc attcgaatcg ccgagctgca ccggtcgtat
     6961 ttcgaacagc cccggctcac gctcaacgaa gacaaattgg cggtcccggt cgcgttggat
     7021 ggcggtttcc ggaatgagca gcacgttggg ctccggatcc gagtacaccc gcaccgttgc
     7081 gtacatctcg ggcttgagtt ttcgttgggg gtttggtagc tcaagtcgga gccgcatggt
     7141 gcgcgtcgca gggtcgagca cgtcccccac ataggtgata ctgccctgga agatttgtcc
     7201 agggtaggcg gagacatgca cctcgaccaa ttggcccgtt cccgtctggt cggtgcggat
     7261 atacggaatg tccttttcag gaattttcgc cgtcacccag acctcggaga ggtcggcaag
     7321 gacgaacagt ttttccgtag tttcgacgac ttcgcctttg gtcagattcc tcgcaataat
     7381 acggccgtcg aatggggcca ccactggaac gtgggatcgg atcgtctggt tcttgtccaa
     7441 gtgccgcaag tcttcatcgg tcaatcccaa caagagcaag cgatcccggg cttcccgttg
     7501 ttcggcgcgg agactgatca tttctccttc acgccgttga gattcggcca gcccgatgac
     7561 cttttccttc aggagcagtt cagcccggcg aaacgcccga tcggcgacgt tcagcttagc
     7621 cgttgccttg agataggcag actgtgccat gcccagctcg ctgctataga ggagggccag
     7681 cagcgtgccg cccttcacct cccggcccaa atctgcatac acgtcgatga cgcgcccgcg
     7741 aacgagggtg gtgatttcgg cgagcgcgtg ttcgtctggt tccaccgtgc cggggaaatc
     7801 ccggaaggtc cggaattcgc cgcgccggag cggttgcacg gtgatccctg ccgatttgac
     7861 tttttcggcc gtcaaacgaa tcgaaccgct ttccgcaggg gcgacaggcg ggtttgcgcc
     7921 cgtggcgttc ggcggcgggc cgtcgcatcc tgacgaggcc agcagtacaa cgaagagcag
     7981 cgaaatgccg cgcgctgtcc accgccgggc gtgcctcggc gaaacctcag cgtggtgatc
     8041 gtgacaagcg gctgccatta caaattccct cccacagcgc gctccaatag cgcgagcgat
     8101 atggaaagat cgaattgagc ctgagcatag tccagcaaaa tctggcgctg gacgcgttgg
     8161 gcgtccagaa cttcgatcag gctggatgcg ccctgttgaa aactgaactt ggcgatccgg
     8221 agagcttctt cagcctggcg gagaagcccc ttttcataga cctcgatgag gtccgccgtc
     8281 gtttttgcat cttggaaatg ttggctgata ttccggatga gttcattacg ggcccgcagg
     8341 tattcggcct cacccttccg cttgcttccg aaggcggaga tgatttcacc ttgccgccgg
     8401 tcccacacgg gtgtcgggaa tgacacgccg ccggtaaacg cttctcgccc gatttcccgc
     8461 cagtagctgc ccccgatcgt aatgttcggc acacgggcct gccgttcgaa ttccagacta
     8521 tggtcggcct gctcgacggc ctttcgcaat cgcatgatcg tgggatgttc tgtcagcgcg
     8581 cgttcggtga ggatgtcgat gccgaatccc gggccgacac ggtgcagctg gccgtcaatg
     8641 gcatagctgg ggccaaggga cccggccgtc agggtatcca gcatgacgcg acccacgcgc
     8701 atgcggttgt tggcgctggt gagcgactgc ttggccttga ggacttccac ttcagaacga
     8761 atcgcttcga actgcgggct ttctcccaaa cgcacccgcg tggtgacggc ccgatgtacg
     8821 tcttcaatcg ttgccaaatt ttgctgcgcg agggtgaggt cgcgttgcgc caggaggagg
     8881 tcgtagaacg cgactttgac gtcggcgatc agattcagcc gggtttccgc caatccggct
     8941 gacgcactcg cgacgccggc ttctgtggca cgctgccgcg cggcgcgttt ggacggccat
     9001 tcgatcggtt gccccacgct cagattgaat tcggtgaccg attcacgcac ggcgggatcg
     9061 aacagcccca gatctcgcat tctgccgcgt ccgctattcg cgttgaccga aggattgagg
     9121 taagtgtggg cggcgacctg ttggccgcgg ttttgttcga tggtcccttc cgcgctcgcc
     9181 actgtcgggt tccgtgccaa ggccagatcc aacacagtgg ccatggaata gaccggttca
     9241 gaggctgaag aggacatggt cgaaggggag gcggcccaac tcagacacgg gctcgaccag
     9301 gccattcccg acagcatcgc caccaggcag ccgatcgtca tgtgatacgt gagacgcagg
     9361 tgccagacat tgacgcttcc tcttgtgaac cgagcctgat cgtttcccca ctgcatcggt
     9421 gatcgcctat tcattgcgaa atcacaatct ccttgtttct gccacgcttg gcgccctctg
     9481 cgtcgtctcg catcgttcct cgtcaacatg tctccctccg caccgtcttg cgccaccgcc
     9541 tgtggcggcg ctgcatcaaa tgcacacggt ctctttcaag taccgtatca gcttgacgga
     9601 gtgaagtcta acgagcgacc cgcgatgtca aggatcagcg agtaatggag gccaggtcag
     9661 tggttccagc gagcaaacag actgtgtggt gtaggagggg actcccgatg actcgtgttc
     9721 tagcgagtcc agtactgtgt gacttacgga gctgacctgg cgaagacact acccggtgcg
     9781 tcggttcgga tgacgcgctc cgtgccactg atagcgggcc tccggttgcg ggcgggcgcg
     9841 agaggcccgt ctcctatacc ggctgacgat gcgattcgag aagggtcgag gttggtcact
     9901 gttggtatct gcatcggtgt ccgaacctgt attacgctca gatccgcttg tggtgcactc
     9961 actcgagtga caccggcgcc acctcgaagg atgttctcga ttgttgttca ttccgcgaat
    10021 gatccttatt cctcgtccgg cccgcatggc cacgtctacg gatgttcttg tgtgagacga
    10081 ccgtagagtt gcgtgcactg cgggcaatat ccgtctgcaa aattcagatc gtgagaaacc
    10141 agattatgtt tctgcatgaa ggtcggcagg tcagaccagg cggctgaatc ggtcccgccg
    10201 gttcgattgt cttcaacccg ccagcaaaac tgacatcgtg tggctgttgg gctcatgttc
    10261 ccgctctcct gcagtagtgt ccggagtgtg gcggaagcgc ctctgatcag gcctcgctga
    10321 tcgccaccac catagcgaat gtggtgcgcg gtcacaacta ggtgatgtcc taggtggagg
    10381 tcagacgcgt cgatgcgacg ggttcgaagg gctgtcgtgt ggcggttgca aaaggtcgcg
    10441 ctaggcggag gtcaatccat gcgccagggc atatttcgtc aactccgcgg tggtgcggag
    10501 attgagatgg aaaataatct gtgctttatg aaactcgacg gttttgggag agatgttcaa
    10561 gagcgccgcg atttctttaa tggtgcgtcc ttcggcaatg agctgcagca cttcacgctg
    10621 gcgcggcgtc aattcctgcc gttgcgcaaa gaggccgctc tgcccagcgg agagcccggt
    10681 cacaatatcc cgcgtcagca gcgcagtcac aaaatactgt cccttcaaga ctgcatcgat
    10741 ggcctggtcc aactctcggg cggcggaacg tttcaagaga taggcggagg cgccggcttt
    10801 gaaggcttca ctgacgtacg ccggatcggc atgcatcgtg acgaagatca gttttacgtc
    10861 cgggaggagt ttcttcaact gtcgtcccgc gtcgactccg ttgagcagtg gcatggaaat
    10921 gtcgagcaga atgagatcgg gacgcagctg catggcggct tcgaggaggg cccgcccgtc
    10981 ttccaccgcg cctacgactt cgcagcgctg ttcaacgatt cgccgaaatc cttccaacac
    11041 cagggtatgg tcgtcggcca ggagtactcg aggtggggtc gtcatacgga aacctccgcg
    11101 cgcggaacgt gaattgagag gcgagtgcct tgttgaggga ttgaatcgat ggtcacggtg
    11161 ccatgaacga ggcgcacccg ttcggccatg ctcaccaggc cgaggccgcg cggattgtcg
    11221 accacctgct gtgtatcgaa tccgacgccg tcatcctgaa cggtgagggt gatcccttca
    11281 gctgtcgagg ccaacgagac ctttgcctgc gaggcatggg catgtttcat aatattggac
    11341 aggcattcct gagcgatgcg atagagacag gtcgacaccg tctgaggaat ggctttcgga
    11401 gacgcatcgg tttcaaatga aatggccagg ttcgagcgga ccgcgcactc gtcaaccagg
    11461 cgctgcaatg ccaccggaag tccgaggtcg tccaatatgg acggatgaaa ttgataggcc
    11521 aagtgacgaa catcgtcgga gagctcggtc aaccggtcct ggatcgagcg aagttctttg
    11581 ctgcagccga cagcggacgc cggaagattg gcttctaacg attcggcctg gacgacgagc
    11641 atggccaggc gctggttgat gtcatcgtgc aggtctctcg agatgcgtcg gcgttcttct
    11701 tcctggacgg tcagcaggcg ggcggcaagg tcgcgcagct cctggcgact ggtcgcgagg
    11761 gcctgttcgc tgttacggag aaatccttct gtttggatgt gcccggaaat ttcctgcagg
    11821 aggctgcgat tgacatcagc cagttccttg gtacgggcat ctactcgcac ctccaattca
    11881 tccttggcct cgcgtagttc aatctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnntggt ccgacaggcg caccacttct tcaatcaatg ccgattcgat atccgccagt
    12061 gtctgttgtc ccagcaccat ctcaatgcgg agcgagggat gttcgccttg tgccatggct
    12121 accgccacat gcgtcgaagc ctgatcgcga agctcacgcg gaaggcaccc ggcggtcaag
    12181 gtcttatcgt gacacagaat catggctcgt tcgatgatat tgtgcaattc gcgaatgttg
    12241 ccgggatagc tatagcgctg cagcaattcc ttggcatcgg gttcgatgtc gatgacttct
    12301 ttgccgaact ctttcccgaa ccgcaacatg gcctgcatgc aaaggggaag aatgtcttcc
    12361 gcgcgtacgc gcaacggagg aagttcgaag gtcacgacgt tcagccggaa gaagaggtct
    12421 tcgcggaagg tgccgcgagc cgtgtctttc ttgagatcac gattggtcgc ggtcatcaac
    12481 cgaaaatcga ccgacaggtc ctccgtgccg cccaggcgcc ggaaggtccg ctcctgcagc
    12541 acgcgcagta atttggcctg catggccggg tctaagtcgc cgatctcgtc cagaaagagc
    12601 gttccccctt ctgccttctc caggagtcct cgcttccgtt ggtgtgcgcc ggtaaaggcg
    12661 cctcgttcat agccgaacag ctcgctttcg aacagctctt tgggaatggc ggtgcagttg
    12721 acggccacga acggaccggc ggcacgtggc ccgttacagt gaatgacgcg cgccagaaat
    12781 tctttcccgc tgcccgtttc tccttggagt aatacggttg atttggcgtt ttcggccaca
    12841 tctcgaacct ggccgagcag cagttgcatc gccggactgc gggcatcgat attggccagg
    12901 gcgtaggcgc tgttctgatc ctcgagtgca aattgaacgc ggtgccgcag cgcgagaaat
    12961 tccaaggcgc gatcgacgac gggatcgacc ccggagagat cgacggactt gatcagaaag
    13021 tcgtaggcgc ccaagcgcat ggcttcgacg gcgttttcga ccgtcccata ggcggtcagc
    13081 atgatgatga gcgtctgcgg ggcaaggggg cggatttttc gcagtgcctc gatgccgccc
    13141 atgccgggca ttttgagatc caggagaatg aggtcggggg cctgctgcgc gacggcactg
    13201 accaggtctt ctccggattc aaagccggta accgtatggt ggcgccgtga aaggcgtttg
    13261 acgatagcgg agcgaatcgc cggttcgtca tccgtaacgt agatcgtggc ctgcatggtc
    13321 cccttcttcc tgcattcgtt gcggctgttg gcgcacgggc gtccacacgc gaacggtggt
    13381 gccgcgtccc tcttcactgt ccagggcgat gtctcctccg tgcgcgtcga gaatattgcg
    13441 ggagatcgcc aatcccaagc ccgttccgcg cggcttccca ctggtaaaga acggttcgaa
    13501 gacgtgtggc agatgactgg gactgatgcc gctgccctgg tccgtcacgg taatttccag
    13561 ccccgattct attccgcgtg cctgttccgc cagggcgacg gtaatggtct cgccttgtgg
    13621 cgtggcttcg atggcgttct gcaggatatt gagcaacacc tgtttaaact gatcgcggtc
    13681 ggcctggatc atatagggag cggcaggggc agaaaggtta atgactgcct gtttccgcgt
    13741 cagaggttcg tccaggacct tcatcacttc atgcaccagc tgtgccatgt cgaaggtaaa
    13801 cgccacgatt tccctggggc gggcatagtc aatgatctgg ttgacgatcc gatccagccg
    13861 tttcgtttcc tggagaatga ccgccaggtc cggccgccga ggatcagacg catcggtgtc
    13921 gtcgagcagc agcgaggtgg ttgagccgat gccgacaagg gggttccgga tctcatgtgc
    13981 aatgcccgcc gccacctgtc cgagagtcgc tagacgctcg gcccggcgca ggcgggtttg
    14041 catgaagtcg agcgcggtaa tgtccacgtt gaatccctga tacatgacga cctgtccgga
    14101 ctgatcacga atcgggatac gccggctcag aactttgcgg atcgtaccgt ctgcatggag
    14161 aaaccggaac acggtttcgt agggttctcc ttccaccacc gcctgggcga attccgcaat
    14221 gacccggtcc cgatcctccg ggtgaatggc acgccgcacc gcatcgggat ctttttcttc
    14281 cgtcgaattc agtccggcaa gcaaactatt gtagcgattg ctgaagacca gcgtcatccc
    14341 tttggtggtg aagatgccga acggagcatg gtcgacgatg ctccgatact tggcttccga
    14401 cccggccagg tcctgattga gctggcgcaa gttgccctcc gattgttgca gggcctcaat
    14461 gtgagaatgg atggccgtac tcatggcgtg catgacccgc gccagattgc cgatttcatc
    14521 cgtcctcgtc atgacgggaa cctgcgggat cgggttgcgg tctgaggtga ccactgccgt
    14581 ggccaggcgg gccagcgggg tggtgatgga atgggcgatc aggtgcaacg ccgccaccat
    14641 gcagatcaag gaaaagagtc cgccgcccag gatggtcatc agcatcgtgt cgcggtcctg
    14701 cgcgatcttc gccagcttgg tattcagcgc agtctgcgtg agatgttcga atcggcccat
    14761 gtcctggcgg atcttcaaca tcagcgcccg gcctttgccc tcctcgatat actcccgcgc
    14821 ttcgttggga tgctcggcct tcacggcatc gatgagcact tccttctcac tgatgaactg
    14881 tttgaccaat tgctgcacag aagtaatgag cgcccgttga tcgtcctgtt gggacacctg
    14941 agcttccaac gtgcggccga tgttcaggac gtggtcttgc gcggtgcgat aggggaacag
    15001 gtaatgctcc tggctcgtca gcacaaagcc ccggaatccc gtttccagat cgaccaccag
    15061 gcgaaggtat tccgaggcca gccgttggga atagtagatg tcgtttaatt gaccttcgtc
    15121 gtcggaaaaa gtcgtgacgc tacggtaggt caagatgctg agcagcagga cggtgagcgc
    15181 aggaatcacc gacgcaagga ataacttgcg ggcgatgggt agattgctta acagatcact
    15241 caaggcagat ctcagtctcc gtcgctatcg tagtctgcac tcccaccact gtgcaacttt
    15301 gcagcctgat tgtcgtcctg agtgggcgct cacgccggtg acccttctta tcgcctgcgg
    15361 ttgttgtttc actccttcct ccagcgttca aagctgccgc cgcgcagagg aatgagcgat
    15421 ttcgcaccgt gcgcccgtgc gatcccgccc gtctccacgc tggtttcacg cgtcatgcga
    15481 ttgtgcgaac ggctgcgctg gcagatgcgg cacgtcacca ttcgttgtcg tggcgatggc
    15541 gtgaacgggc tgttctagat gaggcatgcg ccttgctcag gtcgactatc gtcaagtctc
    15601 ggtgaccggg tcggtgaatc gcggagtgcg cggaatggca gaccgaacaa ggaggagaca
    15661 atggactatg tgaagccgta cggcgtcgtc gacagcatgt tggccggtgg cgcactgaag
    15721 ctgagtttac ccccgcgcca acttgtgctg cgtggcatgt tggccggtgc ctatctcggg
    15781 atcgggacga gcatggccgt gacggtggcc gtcgaaaccg gttattggat cctcggcagt
    15841 ctggtctttc cgttcggatt ggccttggcc attctgctcg gcgcggagat catcaccggc
    15901 agcttcgcgt tattgcctgt cgcggcggca cacggtcaaa agaacgccgg gctctcccgt
    15961 gtctttgcca attggggatg ggtgttcctc ggtaatctcc tcggcagcgt gctctatgcg
    16021 acgttgttcg cgattgcgct gacaacgggg ggcgatgcgc ccatcaatgc ggtcggcaat
    16081 aaattgatcg ccatcgcgca ggcgaaaacg acttactacg catcgcacgg ttccgcgggt
    16141 cttctggcgg catttaccaa gggcatgttg tgcaactgga tggtcagtct cgccgtggtt
    16201 gcagcctata tgtcgacatc gttgtccggc aagtttgtcg cgatctgggg tccgacgctc
    16261 ttgttcttct ctcagggttt tgaacatgcc gtggtcaaca tgtttttgat gccggtgggc
    16321 atgatgctcg gcgcggatgt gagcctctcc acctggtggc tctggaatca gattcccgtc
    16381 acgctgggta atctggtcgg cggcatgctg tttacgggat tggcgattta cgcggcgcat
    16441 cacgcgaccg cgccggctgc cgccgcagct caggcgccgg ctcagcctgt gactgcccgc
    16501 ggcgagcagg cgggatattc cccgtcgtat tagcagtgag ggaacgtcgt catggctgcg
    16561 gtgtatctca ggcgattaca tggatggcga ttcgtgctct tcaatgccgt cctcggcttg
    16621 tcgcacatcg tcgtcttgtt caacgccggc tcctacatcg cgctgttgcc gcatgtctca
    16681 ggggatctcg gcggggtgct gccgagtttt ctgacctggg cgcaaaccga tttcatggtc
    16741 gggctggcat tgggctttcc gctcgcgcgg tacctgtcgg gacggatcgg cgactaccgt
    16801 ctcctgatcg gcgcgttcat ggtgtatagc gtggcctcct atctgtgcgg cgatagtcga
    16861 acgctcgctc aattcgttcc tgcccgtatc gtccaaggca tcgccggtgg catcaccctg
    16921 ccgattgccc aatccatgct tctcaacgaa taccccaaac ggctcaaggc tgtggctctg
    16981 accgtctggg gactgttcag catcacaccc tttaccatcg gcatgccggt gggcggctac
    17041 atcgcctaca tgctcgggtg gcgcttcctg ttttatctgg atttcgtgct gacgctggtg
    17101 attgtcggca cgttgggtgc tctgttgtac ggccgcggct tccgtcgacg gtatgctcgc
    17161 ttcgacctgg tcggatttgc gctgctggcc atcctgttgt tggggctgca aacgttgctc
    17221 aacatgggca acgattttga ctggttggac tcgcccttcc ttcaagtgat cgcggtggtg
    17281 ctgctgattg cctttccctg tttcattatc tgggagttgg gggaacggca tccgacgctg
    17341 gatctccggc tgttcctgca tcgcaatttc gtcatcggga ttatcagcct gacactcggg
    17401 ttcttctcga tccaagggct gttatctctt ctgatcgtcc agatccaact tattctcggg
    17461 tattcctcga agctggccgg tctggcgttg ttgccgttgg tgttgctgag cgccccgatc
    17521 atcgccgtca tgcattatct ctgcaagtat gtcgatgcac gctggctggt ctgcctcaac
    17581 tgtctcggtt ttgcatggac gttttattgg atcggactgt acgacgatcc gcattcctac
    17641 gaagaattgt tctggcccat ggtcgtggag ggaatttttc tgggttcctt cttcacgccg
    17701 atcaccgtgc tgaccttgca cggcctctcg ggtccgttgg tgatgcgcgc cgcagaagtt
    17761 gccaatttgt tgcgggttgc cgccggagca tttgggatca cgtttcaagg catcgtcctg
    17821 ttccggcgca ttcacttcca tcagctgcac ttggccgatc atttcggagg ccggcaatcg
    17881 atttcatacg atccgatggg acagttggcc gcgaaattcc aggcggcagg gttgtctgtt
    17941 tccgaagccc aggccaagtt ggggctggtg atcaagcagg aagcggcaat acttggcctc
    18001 aacgatgcgt ttctcgtggc cagtttcatc tttgtcgggt tgggggggct ggtctggttc
    18061 gcccatccga cgcatgtgcc catcgcctcc agtcctgagg acgatctgcg cgtgagacgc
    18121 gctgaggagt tgatagagga ggtgccatga gatcgggttc aaccgtggga cagcgagtgt
    18181 ggaggtgcct ggggatcggc tgcgtcctgc tcttcgggag ctgcgcgtgg attcccaagg
    18241 gcgatcctcc tgcgcaatat ctcgacccgc cggagatgac ggagacactc gctgaaatca
    18301 cgagccggct gcagaactgg ccggacgatc attggtggga acagttcggg aaccctgagt
    18361 tgaacgggct gatcgaaacc gcgctcaagg acaatcccgg gctgaaacat gcgtccgcgc
    18421 gactgcggca ggccacctcg cttgtaaagg tggaaggcgc ccggttgttg ccgttcctgg
    18481 aagctgatgc ctccttgacc tatgagcgta tctcccaaca tggcgtgttc gccgcgctga
    18541 atcccgaagt ggcgggcatc aagatcatgt acgggatcat caacccgctc agcttccggt
    18601 atgaattcga cttctggggg aagaaccggg cgatgctcga gggagcgctg ggccacgcgg
    18661 ctgccgaaga ggcggaaacc gccgaagtgc gtctgcgatt gaccaccggt attgcccggg
    18721 cctatttccg ggggcaggcc ctgcaacagc aactgacgat cgtgaagacg attgtcggca
    18781 tccgccgcga tctcaaaaat cttgcagaga cccggtttcg tctgggcctc gacaacgatc
    18841 agccgttcaa gatcgccgtc gccgactacg aggcggcatt caagcggcaa gcggccatcc
    18901 gagatcaatt ggatgtgcag cggcatttgc tcgcgcgcct agccgggaag gggccggacg
    18961 aggcagccca tctctttgcc aagcctatgg ccaacattcc caaacagatt cctgcgccgg
    19021 atcatctgtc gatcggcctg ctggtccatc gccctgatct ggcggcagcc ttgtaccgcg
    19081 cgcatgccgc gtcacggttg gtcagggtgg cgacgacgca gttctatccc accatcgatc
    19141 tgacgggctt tgtgggattc aatgccctga cgttggccaa gggaaccgac aagctggcga
    19201 attttctctt tagcggtcag agcttcacct atggcctggc ccccggattg cggatgccgt
    19261 ggtttgaagg cggccggttg cggggcgagt tgggcgcaca gcgcgcggaa tatgacgcgg
    19321 cagtggaact ctataacgac acgctgctcg atgcgatgcg ggaagtagcc gacagcctca
    19381 gtgcctggca caccaccaat gagatcatgg cgtcgcacaa acggctggtg gcgtcgctgg
    19441 gcgaagactg gcggttggca aaagtccgcc tcgtcaacgg cctggacgat gatcgcgaag
    19501 tgctgcgcca ccggtatccg gtgctcgaac aggaatatgc cctgcgagcg ttggaaagcg
    19561 atcaattagt ggccgccgtc gacctgatcg aatccctggg cggcgggtat cacaatccgg
    19621 acatcgaaaa gcgaccgaaa cacaacccga gttaaatgta tgaccaccac aacggcagat
    19681 atgtctcaac ctccgcaatc cccaggagta ccgccaccgg caggctccta tcgcattcat
    19741 ccgaaggcca tacgagcccg ccggaaccgg cgcctattgg tcgtcgctct gctggtcctg
    19801 atcgccaccg tcggctacct catgtattgg tacacccacg atcggttttg gatccggacc
    19861 gacaacgcct atgttaccgg taacctggtg ccggtggcgg cacaggcgtc cggtatcatc
    19921 acgcaggtct tggcggaaga aacacagttc gtgaaccgcg gtgacttgct ggtgcgactg
    19981 gatgaacatc aagcctatgc cgctttggga agggcgcgtg gacgattggg ggaagaggtc
    20041 cgccggatcg cggcgctatt catcaatcgc aaacaactgg cggagaaatt acggtcgcgc
    20101 atggctcgtt tagaactggc cgaacacgac atggaccgct atcaacgggc atccccgagc
    20161 ggcgccatct ccaagcagat cttgcagaat acggcggaca agattgcgag cctggaagcg
    20221 gaggtacggg aaacacaggc ggaactcgac tcgctcgatg cgcagatcgg cggcacgacg
    20281 gtgatggcgc atccgtccgt cgacctggcc aaacaccagt tgattgaagc gcatctggaa
    20341 tatgcacgcc agcagatacg cgccccgatt tcaggctatg tcgccaagcg aaaagcgcag
    20401 gtaggagatc gtgttcagcc cggcgcctcg ctgatgacca tcgttccgct ggatcatctg
    20461 tgggtcgagg ccaatctgcg ggaaacggaa ttgcagcacg tgcggccggg gcaaccggcg
    20521 ctggtgagtg tcagtctgta tggatcgaaa caaacgttcc acggcaccgt tgaaggtctc
    20581 gtgccgggca gcggcagcgc gttcgccttg cttcccccgg ataactccac gggaaacttt
    20641 attcacatcg tcgaacgggt gccggtgcgt attgccctcc cggcggatga actccgtgaa
    20701 catccaatca ggccggggct gtcgaccatg acgagcatta acattaccga gtcggggcaa
    20761 tcggtgtgga cgtctctcgc cacgccatcc acggccgagt atgagacgga cgtgtatgcc
    20821 gatgagcttc ccacagcgga gtctatggcg aaggaagtga tggcgacgaa tttggtggtc
    20881 agcgagaacg gagacggcat tgattcactg ctcgaagacg agggggccat cgagcgggtg
    20941 aaccccagaa gagacgtgga caggttgccg aacgagggga aggcggctcc taaacttgat
    21001 cggcttcgag aacgggatga tcggcgtccg agttcattcg tgccgcgacg cagtcccgat
    21061 ctgggagcca ccacgtttcc tctcacgcca tcgattggac ccgattcgag gtcgctgggc
    21121 cccgaagcgg ggcgcaatcc atctcgactg aaacccgggt tcggtctgga tgacgaacgg
    21181 cgcgcgtttg gttccggcag gcgctagact ccctgccatg acgtcgggcc attccggtta
    21241 ggtgatggaa aagcagcagg ggtagcacag attatttttc gatgaagatt gcggccctgg
    21301 tctggaatgc gcgggctgcg catcctatga tgcaccacgc gctccgagca acaccgatag
    21361 tcaacgctcg cctggcttgc tgatcgttcc ttgcctctcc tgccggcgtc ccgctccaag
    21421 gcgcgctctc agcggtcgca gacagcccct ctaccgggcc ggagacacac gccgtgccga
    21481 acgtgacgct taattatcgc acgctcttgt cggtcaccaa tgtgctgaat tctcagcgta
    21541 accggcaaag cctgttccgt gccatcaccg atcagttgac caaagtagtg cggtgggaac
    21601 gggcaggaat tacagtgtac gacctgcaga cagacgcctt tcgtttttat gcggtggaga
    21661 cgaaccttcc caaagtcgtg ctgcgaagcg atgcgatgat tccgcgcgcg caaagcgctg
    21721 tgggatgggt ctatgaccat caacgcgtgc atgtgcggcc tcaccttcgt gaagagcggc
    21781 tgttcatcga ggatgaaagt tatgcgcagg aagggctggg tcgcatgatc aacctgccga
    21841 tggttgtgca ggaggcctgt cttgggacgt tgaatatcgg gagcatcgaa gccgggtacc
    21901 ccgatcctga cgatgtggat ttccttcagc aggtcgcgac ccagatcggg tttgccattg
    21961 ctcacgtcaa cgcctatgag gaaatcaatc gtctgcggga acaactcgcc cgcgagaatg
    22021 tgtatctcac cgaagagctc aaagcgactc aggactacgg tatcgtcgcg ggacggagcc
    22081 gggcattcga ggcggtgctg acgctggcgc ggcaggccgc cccgacgacc gcgacggtca
    22141 tgattaccgg ggaaaccggg acgggtaagg aggtgctcgc gcgagccatc cacgagagaa
    22201 gtctccggcg tgaacgggca ttcgttcggc tgaattgcgc cgcccttccg tccgggttga
    22261 ttgaaagcga actcttcggc catgaacgcg gcgcgtttac cggcgcggat catcagcggg
    22321 ttggacggtt tgaactggcg gatggcggca ccttattcct cgatgagatc ggcgaaatgc
    22381 ctctcgaggc gcaggcgaag ctgttgcgtg tcttgcagga cgggatggtc gatcgagtcg
    22441 gcagtgcgaa acccattccg gtcgatgtcc gggtgatcgc ggcgaccaat gccaatctgg
    22501 aacgcgcgat tcagcagggg cgattccgta gtgatctgta ttatcgcttg aatgtgtttc
    22561 ccatccatat gccgccgctc agagagcggc cggaagacat tcccattctc gcccgccact
    22621 ttttgcggtt tcacgcccgt cggctgaagc gattgtgtga agattttgat gcgtcctcga
    22681 tggagcgcct cgtgcaatac gcgtggcccg gcaatgtgcg agagcttgaa aacctcgtgg
    22741 aacgcggact gatcctctgt cacgatcgat ggttacgtat cgacccggcc atgatcaatc
    22801 tgcccgccgc ttcagaagtg aatccccgcc ggaccttggt ggacgaagag cgccgccaca
    22861 ttcttcaatc gctcacgttg ctcgattggc gaatcgaagg gcccggcggc gctgctgaac
    22921 aattggggct ggcgccaagt acgctacgca gtcgactaca aaagctcggc attcgtcgtc
    22981 cctcccgttg ctagataccg ttctgctttt cttctcttac gcgtctctcc tccatgggcc
    23041 atgtcattct cgcgccacga ttgatcgtgg tggcgctgtg gtcgctcacc gcggcttgtc
    23101 gtgggtgatg ccttccgtcc gccgcgttcc tgaacgtcat cccgttgaag cgccgatatt
    23161 tcaaggacat cactgacgtc ccatcaacga ggcctgtgtg gcacgcgcag tgcacaaggg
    23221 ctcgtcgtga ttttttctgc cctgattttt ttaatcgaag agcgtagtcc cgcgcgcggc
    23281 ggcctgcgca tcgttgcacc gacgaatgac cgggcacggg tgcgacgaaa tcatcaccaa
    23341 tctggaaggg gggtatgcat ctatcgtcct gtcccggtca atcgtttcat cgggtgcggc
    23401 gccatcacct gtggcgctga tcctgtgctc tgtcttcatt tgaggaggtg ttcatgatgc
    23461 agttatcccg cagacagttc ttgaaagcct cggcaggaac ggtcgcagtg gcagctgtgg
    23521 ccgacaaggc cttggcgctg accgcactgc aaccggtcgt cgaggtcgac aatcctttag
    23581 gcgagtatcc ggaccgctcc tgggaacgtg tgtatcacga ccagtaccgg tacgactcat
    23641 ccttcacctg gtgctgttcc ccgaatgata cccatgcctg ccgcatccgg gcattcgtcc
    23701 ggaatggtgt cgtgatgcga gtggagcaga actatgacca tcaaacctac gaagacctct
    23761 atggcaaccg cgggacgttc gcgcataacc cgcgcatgtg cttgaagggc ttcactttcc
    23821 accgtcgcgt gtacggtccc tatcgcttga aagggccgtt gatgcggaag ggctggaagc
    23881 agtggatgga cgacggctcc cccgagctga cgtcggatgc gaagcgcaag tacaagttcg
    23941 acagccggtt cctggacgat atggtccgcg tctcctggga tacggccttc acctatgtcg
    24001 ccaagggttt ggtcgtcatc ggcactcgtt atagcggtga ggccggagcc cgtcgtctcc
    24061 gcgagcaggg ctatgcgccg gaaatgatcg agatgatgaa gggggcgggc gtgcggacgt
    24121 tcaagcaccg cgccggaatg ccgattctcg gcatgatggg gaaacacgcc aatacccggt
    24181 tcaacaattg tgtcttgccg ctcttggata gctggattcg gaaggtcaac ccggatcagg
    24241 cgcagggcgg acggtactgg aataattata cgtggcacgg cgaccaggat ccctcgcaac
    24301 cctggtggaa cggcacccaa aactgcgatg tcgatttgtc ggatatgcgc ttcaccaaac
    24361 tgaataccag ttggggcaaa aacttcgtcg aaaataaaat gcccgaagcg cactggaaac
    24421 tggaatccat cgagcgcggt gcgcgtctcg tggtgatcac tccggaatat aaccccactg
    24481 ccagccgagc ggattactgg attccagttc gtcccgagac ggacggtacc ttgtttctcg
    24541 gcgcgtccaa aatcattctc gacgagaact atcaggatat cgatttcatc aagggcttta
    24601 cggatatgcc gctgctggtg cggacggaca ccttgcagta tctggatccc ttcgaggtgg
    24661 tgaaggacta tcagttgccg gatttcagca aatcgtactc gggacgattg caaggattga
    24721 ctcaagatca gatccgccgg ctgggcggca tgatggtctg ggacttggca aaggggaaag
    24781 ccgtgccgtt acatcgcgaa caggttggcg tacatcttgc gcagagcggc atcgatccgg
    24841 cattgaccgg aacgtatcgc gtcaaattgc tcaatgggcg cgagatcgat gcgataccga
    24901 tctatcagat gtatcaaatt catctgcagg actatgacct cgatacggtg catcaggtca
    24961 atcgggcgcc aaaagatttg atcgtgcgct gggccaggga ctgtgggacg gtcaaaccgg
    25021 cagccatcca taacggtgaa ggcgtctgcc attatttcca tatgacctcg atgggacggg
    25081 cggcagcgtt ggtcatgatg ctgacgggta acatcggaaa attcggcacc ggctgccaca
    25141 cctggtccgg taactacaag gtgggtatct ggcaggcggc gccctggtcc ggcgcaggcg
    25201 cgagcgtcta tttgggggaa gatccgtgga atttgaatct caaggatgac gtacacggca
    25261 aggagatcaa gtaccgcaaa tattattacg gtgaggagcc cggttattgg aatcatggcg
    25321 acaatgccct gatcatcaac acgccgaagt acggccgcaa ggtgttcacc ggcaagaccc
    25381 acatgccgag ccccagcaag gttcggtggg tggtgaacgt caacatcctg aacaatgcga
    25441 aacaccacta tgacatggtg aagaacgtcg acccgaacat cgagatgctg gtcacgcaag
    25501 acatcgagat gacctcggac gtcaaccatg cggatgtggc gtttgccgtg aattcctgga
    25561 tggagttcac gtatccggag atgaccgcga cggtgtccaa tccctgggtg cagatctgga
    25621 aaggcgggat ccgccccttg tatgacacca gaaacgatct cgacagcttt tcgggtgtgg
    25681 ccgccaagct caaggagatg accggcgaac agcggatggc ggatacgtac aagtttgtct
    25741 atcacaatcg tgtggatgtg tacgtgcagc gtatcctcga tgcctccagt accttcttcg
    25801 gctatagcgc ggacgtcatg ctgaagtcgg aaaagggctg gatggtcatg tgccggacgt
    25861 atccgcggca cccgctctgg gaagaaacca acgagtcgaa accgcactgg acgcgatccg
    25921 ggcggctgga aacctatcga gtggaacccg aggcgatcga gtacggagag aacttcatct
    25981 cccaccgcga aggcccggag tgtaccccgt atctgccgaa tgccatcatg acgaccaatc
    26041 cctatgtcag gccggatgac tacgggattc cggtcacggc gcagcaccat gacgataaga
    26101 cggtacggaa catcaagctg ccctggtctg agatcaagca gcacgcgaat ccgttgtggg
    26161 agaagggcta tcagttctac tgtgtcacgc ccaagacccg ccaccgggtc catagccaat
    26221 ggtcggtgaa cgactgggtg cagatttatg agtcgaactt cggcg
//
LOCUS       DAOQEW010000031        24763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_474_length_24763_cov_7.704104, whole genome shotgun sequence.
ACCESSION   DAOQEW010000031 DAOQEW010000000
VERSION     DAOQEW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24763
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_24763_cov_7.704104"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLS35_05370"
     CDS             complement(<1..511)
                     /locus_tag="PLS35_05370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNI18118.1"
                     /translation="MKTILITGGAGFIGSNFVRYLYDKYPNYRLIVLDALTYAGNVKN
                     LPVDINNGSDERLMFWYGNVRNAELVDTVVSQSDVVMHFAAESHVTRSIYDNLLFFET
                     DVLGTQAVANAVLKYRDKVERFIHISTSEVYGTAESEKMSEEHPLLPMSPYASAKCGA
                     DRLVYSYWQT"
     gene            complement(525..1235)
                     /locus_tag="PLS35_05375"
     CDS             complement(525..1235)
                     /locus_tag="PLS35_05375"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNI18119.1"
                     /translation="MHDPNLQSSITLCVLQPGYLPWLGFFDQMRRSDIFVLYDDVQYD
                     KHGWRNRNRIKTPKGPHWLTVPVRHKGLGWPRINEVEIDNQTVWGKKHIGTIRQFYAK
                     APYLAQYLPMLEDRLLRPWTLLVDLDLSLIDLMCEWLQLKRDVIRASSLGIEGDRSDR
                     LLALCRHFGATRYLSGDAAKTYLDRELFAQNGIEVEWQGFQHPVYPQQHGEFVPFLSA
                     LDLVLNCGPDSAAILRGQ"
     gene            complement(1287..3143)
                     /gene="asnB"
                     /locus_tag="PLS35_05380"
     CDS             complement(1287..3143)
                     /gene="asnB"
                     /locus_tag="PLS35_05380"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNI18120.1"
                     /translation="MCGIAGRFGPGPNQAVKKMVALLAHRGPDDEHVVSGRNFAIGAR
                     RLSILDLQGGRQPISNERETIWAAQNGEIYNFPALRNELLERAHVLKTHCDTEVLPHL
                     YEEHGAAFPKKIDGMFAVALWDGDGQVGYLARDRTGKKPLYYCLHDQALYFASEIKSL
                     LQIPGFTRRLNLDALHQFLSLKHVPHPLSIFEGIAMLPPAHMLTYRAGQEPVVSRYWD
                     LSFAGSDTSAGMTEEEITDRLLVLLRQGVERRLLSDVPIGFFLSGGIDSSLSTVLAAE
                     LSPSKIKSFTLTYSRESTTEGKEEDRRWARWVAEQYGTEHHEETIQVSSFPENLRRIL
                     TCFDEPYAGVVSTYFLAQLMSQHVKVALSGDGADELFGSYLSHRLAVPLANFQTYLQT
                     GNSALIRPFEQQVDFLARMADPQDWGWRYKLLVFSDEEKDALYSEEIASHMRSYSTRD
                     RLRHDFSELSARDPLNRILESEFRTIFPDQVLAFVDRLSMAHSLEVRTAFLDTDFVEF
                     VAGLPGSLKIKDGETKYLLKKLGLRYFPREMVYRKKEGFLMPITQWLLRDLESYVRET
                     LSPERLAKHGLFRAAYVQRLVDALYQAQPDHLAVNKVYALVVFQEWYDLYMV"
     gene            complement(3149..4642)
                     /locus_tag="PLS35_05385"
     CDS             complement(3149..4642)
                     /locus_tag="PLS35_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18121.1"
                     /translation="MEMFFAPQGFLHPELSLSVQMFMRVVYGMLLCGMLLLAIPNWRR
                     FFLSERWGGYAQSLREVEFIQNPLVFPLVMAVWFACGVCITGGWWTPWPALLNLVLCR
                     YFFVGMRWKGLLRGMGAPGYILYWGGLAITVLEYTLHYAPELRSLALQVLQADFACVY
                     ISSGVYKLSAGYAKNHGMELGMTNPAWGYWWKHYQHFSPSHPLFRFLNHLAWSSQIIA
                     GILMLLPPTRFIGGMIIFGMFAFIATQIRLGLLCEIVMFCSSLIFLHPGSLGDIVVTA
                     VVGQVQSPSVASFAFSSVANTVLGWALAIYLILLPFAHVGLSYNFYLRRSLPKPLQMA
                     LERYTNFFGLIVWRVFSVDIVNFFIRIYHYPKAHESDRQLISDESVGARLRFGHVAES
                     IVLACIFTTLKYYPHNSGLFHERLLRYARTFPCEKGELLIFEYLSLRKEPDRFVFAPV
                     AEYTVDTAAMTVVERVIDPSVSVRAPHAVSPVHEGVRPGTYAPAGGR"
     gene            complement(4714..5148)
                     /locus_tag="PLS35_05390"
     CDS             complement(4714..5148)
                     /locus_tag="PLS35_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HNI18122.1"
                     /translation="MSMKERVYVIYDGQCRFCIRSLKLFSAADIFKVFRFYDAHDEQS
                     VAALFPELQGADFDNAMFVVTERREVYRGFFAFRRMIWSSPLLWPLIPVFYFPGATPV
                     GTRLYAWVAKNRLKFGCQSDACTLPARLPKDPSSDHVDVVPH"
     gene            complement(5172..6131)
                     /locus_tag="PLS35_05395"
     CDS             complement(5172..6131)
                     /locus_tag="PLS35_05395"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNI18123.1"
                     /translation="MSEKNVVQNKAEAHRGRVIVFGYGALAAAAVTALLSAGAEVIAV
                     VAPSNRSGDDVDFIKDFACTAGIPVLVQPPRKTIAPFVQDLKRLDPDVIVVWSYPMIL
                     PPDVIEVPREGTVNVHGGLLPEYRGGHVMQWAIINGESETGVALHYMDEGIDTGPVIA
                     QRRFPIERDDDAATVREKLKEAGMALLKEWWPAISQGRAPREKQDEATAKYHRLRTID
                     DGLINWHASATEVCNLVRALVRPWPGAFTILNKKKLVIWKCRLLVQETSGVPGLVVQV
                     DAQGARVCTRDRDVLIETAELDGERMTGLDLKRIMQAGTCLGA"
     gene            complement(6160..7278)
                     /locus_tag="PLS35_05400"
     CDS             complement(6160..7278)
                     /locus_tag="PLS35_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI18124.1"
                     /translation="MTSKPLVVVPDEKTYGLKPGAEEFPLMVIISIIYPCNMGCPNCP
                     YTDTNSELRRFYHEHDGDLIPVKLWEKIADECGPYGAWMRCTGGGEPMLHPKMVEMIE
                     YAKAKGARVWLNTNGSMFGPNAKHRERLERVIRAGIDLIEFSMDAADAETYSVLRPPR
                     SGKTGDPEQWWTKHVGNVRAALDLRKQHVATTRVVTSIIRQEAIEGRLDDAVQFWLKD
                     VGVDEVITRKFLSWDDNTTIQLGKTLDRHLYADLPKQKKEPCVWPFERLNVDTLGRIA
                     LCGQDISFKTSELFPNVKDTSIKEIWQGEMFTWYRRMHLEGRGAECFPCRGCSAWFAG
                     IRDWDHGWQKVLRNSGDHLKEVMRRDLGVEVEVFQPKV"
     gene            complement(7572..8510)
                     /locus_tag="PLS35_05405"
     CDS             complement(7572..8510)
                     /locus_tag="PLS35_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNI18125.1"
                     /translation="MEKQARIFVAGHQGMVGSAILRALTSRGYERLIVRASGELDLRD
                     NVRVAAFFEEVKPDYVFLAAAKVGGILANSTLPAEFIYDNLAIQTNVVHQAYVHGVKK
                     LLLLGSSCIYPRDSPQPMKEDYLLTGPLEPTNEGYAVAKIAGIKMCQAYRQQYGCDFI
                     VAVPTNLYGPNDNFDLQHSHVLAALLRRFNEAREQGGEPTLWGSGTPLREFMHADDCA
                     DACLFLMERYSHAEIVNVGAGEGISIGRLATLVAEVVGYRGDIHWDPTKPDGMPRKLM
                     DCGRMKALGWTPRIPLREGLQDAHRWYQGHVKRIAD"
     gene            complement(9010..9867)
                     /gene="rfbD"
                     /locus_tag="PLS35_05410"
     CDS             complement(9010..9867)
                     /gene="rfbD"
                     /locus_tag="PLS35_05410"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNI18126.1"
                     /translation="MRIVITGAQGQLGKELQQVLQGHQLTLLDLPTFDLTHEDCGRLI
                     VDAVPDAVIHAGAHTDVDGAERNPQLAMAINADGTERVARAAAQVGARLIYISTDYVF
                     DGRGTRPYVETDQTNPVSAYGASKCAGEARALACCENTLVVRTAWLYGLQGKNFVKTI
                     LQLAAERPSLRVVADQEGCPTFAEDLARMIDRLVTHPIRGFLHVTNEGHCTWHEFAAE
                     IVKLAGYRVPVQPITTEDMPRPAKRPAYSVLSSDRLHHLGFSMPSWQNGLQRFLKALP
                     ALSSMSSRT"
     gene            complement(9852..10883)
                     /gene="rfbB"
                     /locus_tag="PLS35_05415"
     CDS             complement(9852..10883)
                     /gene="rfbB"
                     /locus_tag="PLS35_05415"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNI18127.1"
                     /translation="MRILVTGGAGFIGSHLVRRLVQSGRHSVVNLDALKYSGNLENLT
                     DLVDHPQYRFIHADIGDQKAVHAALQEHRIEGIINCAAETHVDRSILDPGAFARTDVV
                     GTGILLEEARQAGVQRFLQVSTDEVYGSVEQGSSTEGDRLEPRSPYSASKAGGDLLVL
                     SYWTTYRFPVVVTRGSNTYGPNQYPEKFIPLFATNAIDGEPLPLYGDGKQCRDWLSVY
                     DHAAGIQHVFEDGQPGTVYNVGGGNERENITVAEQIVAALGKSRSLIRFVQDRPGHDR
                     RYSIDCSRLRALGWSPQVPFEEGLQQTVEWYRTHEDWWRKIKSGEFKAYYQQQYGQRL
                     AKGTACGSS"
     gene            complement(10883..11617)
                     /locus_tag="PLS35_05420"
     CDS             complement(10883..11617)
                     /locus_tag="PLS35_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNI18128.1"
                     /translation="MKGVVLAGGLGSRLLPLTKVTNKHLLPVYNKPMIYYPIQTLVNA
                     GITEIMLVTGGNSAGDFLKLLGNGRDFGLKHLSYTYQQGEGGIADALRLAEHFADDGP
                     ICVVLGDNLIQGNIAKAAEAFRTQKKGAKILLKEVKDPQRFGVPHLDGNQVLSIEEKP
                     AHPKSSYAVTGIYFYDARVFEITRTLKPSGRGELEITDVNNAYIASGELTWNVLDGWW
                     TDAGTIESLLAANQLVAQTGANRMEF"
     gene            complement(11765..12847)
                     /locus_tag="PLS35_05425"
     CDS             complement(11765..12847)
                     /locus_tag="PLS35_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNI18129.1"
                     /translation="MKIVIAAWHLKNFNVGIGRYARELIEALGSVDQTNHYDILLPQP
                     EHPFTARPNMRYRVVRFPLFRRRFWEQVAPLMVGPYDVLHFPYDSCVAWKRGKFVTTI
                     HDVKPLLFPELRPRTNLNSRIEDWLVGDRWQTIDQVITVSEHSRRDLLAHVPLRPDQV
                     SVTPLGLDAGRFRPAEHRHEGRPYVFCVAGSDPTKNVGVLVEAFATLPADLRNRFDLI
                     LAGDVCKREDIRAAIARRGLGAQVKLVGLLSDADLVTYYQQATVFVFPSLYEGFGLPV
                     LEAMGCGCPVICSNASSLPEVAGEAAVLVDPRRTDQLTQELARVLESIELQLSLRERA
                     LARSKEFVWERTAKQTVAVYERAVGR"
     gene            13027..13902
                     /locus_tag="PLS35_05430"
     CDS             13027..13902
                     /locus_tag="PLS35_05430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNI18130.1"
                     /translation="MAHVSVVIPAYNGTSRYLNEAIRSVQAQSFADYEIIVVDDASTD
                     RTDALVQAIPQVRYVRHSKNAGQAAARNTGAQLAQAPYLAFLDQDDLWEPTFLERTLA
                     ILEPATGAVLVHCDGYQVNERNDILEYDAAMKHQRSITQLLRGGHDAATSGSLFRKSW
                     FDSVGGYDAALSIWEDIDLAIRLYGPERFIHLPEPMYRHRLYGHNASRDIPSLRALDG
                     RRRFLEKHAATCRPGTPEGEALSNDWAIYYADLGKHHLRAHARQEARQAFRFALRHQP
                     LNFKTWSRLLRSYFV"
     gene            complement(13883..14920)
                     /locus_tag="PLS35_05435"
     CDS             complement(13883..14920)
                     /locus_tag="PLS35_05435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI18131.1"
                     /translation="METIDCEVCGGTSTTTMFSQYDLTHRVTDDVFTVVRCLDCRQLF
                     LNPRPTRQEIGRYYPDTYYPDAAPRQAGDLRRAAKRWSATIRRWIAQDFYGYPAQEQG
                     RAWQRVRRMFLWPEFLWRCWRGRGLLPWVGQGRVLDVGCGAGGNLASLQEQGWEVSGV
                     DASAVAVTQAKACFGDRVRLGDLEAIAYPDRSFDTVLFSHVLEHLHGPLPLLKEVWRI
                     LDQDGRIVILCPNAGSLEAKIFGRWWFPWELPRHLFHYEKATLRRILEAAGFRIESMR
                     TGLGSLYFMASLDRVCEQRFGRAIPCRRLLEKSLIRPFCFCVGHLGYGTELKVYARKD
                     ASLNQATQSTI"
     gene            complement(14952..15848)
                     /locus_tag="PLS35_05440"
     CDS             complement(14952..15848)
                     /locus_tag="PLS35_05440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNI18132.1"
                     /translation="MNESVSVVIPVFNGAPFVAKAVASVRAQAHGDVEILVVDDGSTD
                     GTQEVLKRLEESDGIRWFQRSHGGPARSRNFGIEAARGQYIALLDCDDVWLPGKLAAQ
                     LAIMRTRPEVGLVHTDFDVRFEDGTLEERVIARSSREPMVQAFAGGHVALPSTLLIRK
                     SVLDQVGRLDPELYGSEDSDLTIRLFRVTSFECIDQVLVTKLQRGHGYRDMAFDEQTH
                     RERVLASRDRFLMRLEGFAPLTTAQRAALDREWANYYLLKGAAAERAGRPAEARSQYR
                     KAIGKAPGRIRCYTRWLRTWTS"
     gene            complement(15845..16903)
                     /gene="rfaQ"
                     /locus_tag="PLS35_05445"
     CDS             complement(15845..16903)
                     /gene="rfaQ"
                     /locus_tag="PLS35_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HNI18133.1"
                     /translation="MIATGLLRPKNILIIKLRHIGDVLLSTPVLRALREAFPAAQLTM
                     LVNRGTEGVLAHNPDVNEVLCLEKGAWADQCKFVYRLRERGFDCVIDLTDGDRSAVIS
                     LATGAPIRIGFNAEHRWRGLLYSRVAKPRPVDQHRVEYDLCALRALGLDPKRGMPSVS
                     VSDSEEQVTDQWLTEQGLIGDQCPPLVLLQPGARYSLKVWPPERFAQLSDRLVEQFRC
                     RILLGGDQREREVAEQVARKSHCAPIVVAGKFSLLQYASLVKRCALFVGNDGGAMHIA
                     ATVGTPVVALFGPTYPERWGPRGGLTQVIYKGLDCRACYHPSCLRGDDSCMRQITVDE
                     VFEAAGRILDLKGARVVT"
     gene            complement(16900..18003)
                     /locus_tag="PLS35_05450"
     CDS             complement(16900..18003)
                     /locus_tag="PLS35_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNI18134.1"
                     /translation="MRIGIDASSLVGDKGGVGWHTYHLLKALLQLDEKTDYVGYLRPG
                     SLQAGQLSGWPSRSRMQWMETPRWLMSWRGRWDRLDLYHGPNFKMHTTGRYGGIVTIH
                     DLWLARHPEYSRKLFGQAGSSRRAIATAKRARKVVTVSEFSAREIESLYGIPRDHVRV
                     IYNGVSEDFTPLCDDQIRAEVRTRWSIPSSGFILFVGGADPRKNHVGFLQAVGACRSQ
                     LGGRAIVLVGDAEHPQGSYTATAQSLGLEQEVRCTGRLDREDLRRLYSCADLFVFPSH
                     YEGFGMPVLEAMACGAPTVTSSTSSLPEVAGDAALLVDPNNVEELAHAMVTVLSDTHL
                     RENLRQRGYERARLFTWQRAAVSTSALYRELCT"
     gene            complement(18053..18814)
                     /locus_tag="PLS35_05455"
     CDS             complement(18053..18814)
                     /locus_tag="PLS35_05455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI18135.1"
                     /translation="MTIAAVVITKDEEHNIAECLQSLRWVDELIVVDAESRDRTVELA
                     KGYTSKVFVRPWPGYGPQKNFGIDQAGADWILVVDADERVPDALRQEIQALVDSRPPA
                     DIGGYEIPRRNVFYGKWIQGGGLYPDYQLRLFRKTAGRYDDVRLHENLALSGRRERLR
                     EPFIHYSMPTVNHHIRKMMRYTTLGADEKLKRVRHISGWTIATHHLGTILKTLFTRGG
                     YRDGVHGLVVAMFAGLHTFVKYAKAWERLNVRRDA"
     gene            complement(18845..19642)
                     /locus_tag="PLS35_05460"
     CDS             complement(18845..19642)
                     /locus_tag="PLS35_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI18136.1"
                     /translation="MDRIPVSAVVIAYNDEPNMRACLESITWADEIIVVDSHSTDGTE
                     RISREFTDKVYQHDFHGFGRLRNEALAHATHDWVFSLDTDERATPELRDEIRRLLDAG
                     PEADAYFVPRKNYFLGRWIKHCGWYPDYRQPQLFRKSKLRYRDELVHESFDLDGRLGY
                     MTAAALQYPFRDIDHYLAKQERYSDLMAKRMVEQGRAFSWHQLVSHPCFTFLKMYVGR
                     KGCLDGVPGLILSGLYAYYTFIKYARFWELQSQGVRQPNRVAPAKQG"
     gene            complement(19699..21840)
                     /locus_tag="PLS35_05465"
     CDS             complement(19699..21840)
                     /locus_tag="PLS35_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNI18137.1"
                     /translation="MMPRWADSGASIRKSVLFWWILFVVIQMAERVFLLRDALDQETP
                     TASLLLKTLLVGVRGDFITATFALVLGGVGAGLYSLLRQGVAGFRQSPRRFSLSFRPA
                     FHVGCLLAGLLLFVLLCVDMGYYGFNHQHMDFVFLEYIGDLLAPAATTEGTNVQAMQQ
                     TSAELEAAGKWAWRLTVFLAIQAAGIGLWWWSFSRAVVPALDRWRPGSGFQANALLVV
                     ALVAGGAGFHHSGPYGIRIAPIGSMVYYTLAQNPVLFAAEALRIAVVSRNPIERRANT
                     EAMPYDLAVRTAQQLLGPGETFVDPRYPFVRTTSPSPDGVRLSKPANILLVFLEGLDR
                     RYLGQTYGEVKGTPFLDRLKQDSVYFQNFFSNGVQTSRGLFATLCSTFPRQGAAAMKT
                     RYAHDYLCLPSLLQRQGYSTEMVIGQHRDLNRLQTFVARNGLQQLLDEGDFPANTERA
                     GLGIVDGALFDLCYERIKLRQSQGKPFFLSTLTLSTHHPFAAPMNHPEVQLLQQQVQD
                     KYVAALRYTDLELERTFTRLVREGLLRNTIVVILGDHGRHEPVGGSDIERKVGHFASP
                     LFLWMDESLRTPATYRPRVVDTIASQVDVAPTLLSLNGGLPAMGAFAGHDVSCALVQD
                     CLPGQVAYLTSVYDNLVGLASAEGLLLYSFATETFQEATLRFEPVPDEQTGGRRLSVS
                     RDRELMALYEVANVAMDSNRLWSWREFGSRL"
     gene            complement(21837..22940)
                     /locus_tag="PLS35_05470"
     CDS             complement(21837..22940)
                     /locus_tag="PLS35_05470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI18138.1"
                     /translation="MNILIAESSMAVGGQELAVLLHAERLVARGHHIRLVLEPRSPIM
                     AMAQERGLPVEPFVMRQWRLPLSILAFRQLLTRERPDIVHVNSSRDSWIAALSTRLLS
                     PRPKVIRTRHISAPLNNNATTHLLYRRLFDMVIVTGGERNRQDLIHRDGLSPDRVAAF
                     PIGLDVEQFSPAQPTHDIRAELGIPPGHLLVGMISYLRDYKGHRYLVDAAAKVLKQHP
                     AAAFLIVGEGPEEQNIRAHIERLGLAADVRMLGFRDDLLDVFRSLNLFVIPTVEGDTI
                     PQVLMQALAVGLPVVSTTTGSIPDVIADGESGFLVPPRDPEALADRIVRLLKDANLRA
                     AMGKRGRHTVEQSYSIDRMVDELERVYRKVIAS"
     gene            complement(22937..23980)
                     /locus_tag="PLS35_05475"
     CDS             complement(22937..23980)
                     /locus_tag="PLS35_05475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNI18139.1"
                     /translation="MTEPVRRILLIKPSSLGDIVHAMPTLAALRERFPQAEVTWLVKR
                     QWASFVEVIKGVDHVCAVEQGLRGWLGRVPDLRAARFELVVDLQGLFRSGAMAWLTGC
                     SRRVGFANAREGSPFLYTQRVAVPNGSMHAVDRYLLVAEALGAARQTDPRFDFADRAE
                     DRGAAEALLTAAGIRVSAPWIAMNVSARWETKRWPAQHFAEAADLLAEAQHIPVIFMG
                     GPAERSETRAVMARMRTKAVDLTGQTPVGLLPSLLRRAAVFVTNDSGPMHIAAAVGTP
                     VVALFGPTDPVKTGPYGQGHVVLSNPVECRPCFRRECSRAATLECLTGVTPEQVVRAV
                     QRQLGRLDTSGSS"
     gene            complement(24005..>24763)
                     /gene="gmhB"
                     /locus_tag="PLS35_05480"
     CDS             complement(24005..>24763)
                     /gene="gmhB"
                     /locus_tag="PLS35_05480"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNI18140.1"
                     /translation="RQPVSCAPCLLRECPIDHRCMTGVTVEQVHDAAARHLPLAAPPF
                     LSAPAVVTPALSPTALAGVTVFLDRDGTLNVDTGYVKTPEEFTLLPGVGAALARLKGG
                     GAKLVVVTNQSGVGRGYFSSKDLEAIHNKLRLLLAEDGVTLDGLYFCPHHPDDRCNCR
                     KPSRGMVDRALAELQVDLSRAYVVGDSVRDVELAKQVGARAVLVMTGPSGAEALTDLT
                     ARDLSPDHVAADLSHAVEWTIAHATRKPVADVTG"
BASE COUNT         5631 a         7719 c         6495 g         4918 t
ORIGIN      
        1 aggtctgcca atacgaatag accaatcgat cagcgccgca cttcgccgat gcatacgggc
       61 tcatcggaag caatgggtgc tcctccgaca ttttttctga ttccgcggtc ccgtagacct
      121 cagatgtcga gatatgaatg aatcgctcta ctttatcacg atatttgagt acggcgtttg
      181 caacagcttg cgtgcccaac acgtcggtct caaaaaagag caagttgtca taaattgacc
      241 gcgtgacatg tgactcggca gcaaaatgca tcaccacatc cgattgggat accaccgtat
      301 caaccagctc ggcgttccgc acatttccat accagaacat cagtcgctcg tcggaaccat
      361 tgttaatgtc caccggcaga ttcttcacat tccccgcgta ggtcaaggca tccaacacga
      421 tcaaacgata gttcggatac ttgtcgtaga gatagcgcac aaaattactg ccaatgaaac
      481 ccgcccctcc ggtaatcagt atggttttca ccaatcctcc tttttcattg gccgcgtaag
      541 atggccgcgc tatccggccc acaattcaac acgagatcga gagcagagag aaacgggaca
      601 aactccccgt gctgttgtgg atacacgggg tgctgaaaac cctgccactc aacctcaatt
      661 ccattttgcg cgaagagctc acggtccaga taggtcttcg ccgcgtcacc gctgagatac
      721 cgtgttgcgc caaaatgtct acacagtgcg agcaatctgt cactccgatc tccttcaatg
      781 ccgagcgagg atgcacggat gacgtcgcgc ttaagttgca accactcaca cattaagtcg
      841 atcagagata aatcaaggtc aaccagtaac gtccagggcc gtaacagtcg gtcttccagc
      901 atcggcaggt attgagccaa ataaggcgcc tttgcataaa actgtcggat cgttccgata
      961 tgctttttcc cccagactgt ctggttatca atttcaactt cgttgatacg tggccaccct
     1021 aatcccttat ggcggacggg gacggtcaac caatgaggcc ccttcggagt cttgatacga
     1081 ttccgatttc gccatccgtg cttgtcgtac tgaacatcgt catacaggac gaagatatca
     1141 cttcgacgca tctgatcaaa gaagccaagc cacggcaaat agccaggctg aagtacacac
     1201 agcgtgatgg atgactgaag attcggatcg tgcattattc aggcaggaat actcgcactc
     1261 ttagcgttag attcgaacca tggaccttaa accatataca agtcatacca ctcctgaaac
     1321 acaaccaatg catacacttt gttgacggcg agatgatccg gctgcgcctg gtataacgca
     1381 tccaccagcc gctgaacgta ggccgcacga aacaacccgt gtttggccag tcgctctggg
     1441 ctgagggtct ctcgaacata tgactctaga tcccgtagca gccattgcgt aatcggcatc
     1501 aaaaagcctt ccttcttgcg atacaccatc tcgcgcggaa agtatcgtag tcccagcttc
     1561 ttgagcaagt acttggtttc tccgtctttg atcttcagcg atcccggcag tccagccaca
     1621 aactcgacga agtcggtatc gagaaaggcc gtgcgaacct caagtgaatg agccatcgaa
     1681 aggcgatcca caaaggcaag aacttgatca gggaagatag tacgaaattc cgactcaagg
     1741 atccgattca gaggatcacg cgcggatagt tcagagaaat catgccggag tcgatcacgg
     1801 gtgctgtacg atcgcatgtg tgaggcaatt tcttcgctgt agagcgcgtc cttttcctca
     1861 tcactaaata ccagcaattt gtatcgccat ccccagtcct ggggatcggc catccgcgca
     1921 agaaaatcca cctgctgttc gaaaggccga ataagtgccg aattcccagt ctgcaggtag
     1981 gtctgaaaat ttgccaacgg aacggctaga cgatgagaga gatagcttcc gaagagttca
     2041 tcggccccgt cacccgagag tgccaccttg acgtgttggc tcatgagttg agcaagaaag
     2101 tacgtcgaaa ccactcccgc atagggctca tcaaaacatg tcagaatgcg gcggaggttt
     2161 tcgggaaaac tggacacttg gatcgtttcc tcgtgatgtt ctgttccata ttgttccgcg
     2221 acccaacgag cccaacggcg atcctcttct ttcccttcgg tcgtcgattc ccgcgaatac
     2281 gtcagcgtga aacttttgat cttggaaggc gagagttccg cagcaagcac cgtcgacaga
     2341 ctggaatcaa tgccaccact cagaaagaac ccgatcggta catccgacaa cagacgccgc
     2401 tcaaccccct gccgaagcag gacaagaagc cggtcagtaa tctcctcctc cgtcattccc
     2461 gcgctcgtgt cgcttcccgc gaagctcaga tcccagtagc ggctgacaac tggttcctgt
     2521 cccgcgcgat acgtcagcat atgcgcaggg ggaagcatgg caatgccctc gaatatcgaa
     2581 agaggatggg gcacgtgctt caaactgaga aattggtgca gcgcatcaag attcaagcgc
     2641 cgagtgaagc ccgggatttg caataaggac ttgatctccg acgcgaagta gagcgcctga
     2701 tcgtgcagac aatagtagag gggcttcttt cctgttcgat cgcgggccag atagccgact
     2761 tggccgtcac cgtcccacaa ggcaacggca aacataccgt ctattttctt tgggaatgcc
     2821 gccccgtgct cctcgtacag atgaggcaag acttcagtat cgcagtgggt cttgagcaca
     2881 tgtgcacgct cgagcaactc atttcgaagt gcaggaaagt tgtaaatctc tccattttgg
     2941 gccgcccaga ttgtctcccg ctcatttgag attggctgcc gccctccctg aagatcaaga
     3001 atgctaagcc gtctggctcc gatggcgaag tttctccccg acaccacatg ttcatcatct
     3061 gggccacgat gggcgaggag cgcgaccatc tttttgacag cctgatttgg gcctggacca
     3121 aaccgtcccg caattccaca catatccttt aacgcccccc tgccggagca taggtccctg
     3181 gccgcacccc ttcatgaacc ggtgagaccg catgcggcgc ccgcacagat accgaaggat
     3241 cgatcacgcg ttccacgact gtcatggccg cggtatccac cgtatattcc gcaaccggcg
     3301 caaacacaaa tcggtccggt tcttttcgta gactgagata ctcaaaaatc aacaactcac
     3361 ccttctcgca gggaaatgtt cgtgcatatc gaagcagacg ttcgtggaac aatccgctat
     3421 tgtgaggata atacttcagt gtcgtaaaga tacaggcgag cacaatcgac tctgcaacgt
     3481 ggccaaaccg tagtcgtgcc cctacacttt cgtcggaaat aagttgtcga tccgattcat
     3541 gcgctttcgg atagtgataa atccgaatga aaaaattgac gatatcgaca gagaatactc
     3601 tccaaacgat caatccaaag aaattcgtat agcgctccag ggccatctgg agcggttttg
     3661 gcagtgagcg ccgcaggtaa aagttatacg agaggcccac gtgtgcaaac ggcagcagga
     3721 tgaggtagat ggctagtgcc caacccagga ccgtattggc cacagacgag aacgcaaagc
     3781 ttgcgacaga gggagactgg acctgtccga ccacagccgt aacaacgata tccccgagac
     3841 tgccaggatg aagaaaaatg agactgctgc agaacatgac aatttcacac aagagcccca
     3901 gcctgatctg agtggcgatg aacgcaaaca tcccgaatat gatcatgccc ccgatgaatc
     3961 gagttggggg taagagcatg agaatgcctg cgataatttg tgaagaccag gcaaggtgat
     4021 tgaggaaccg gaagagggga tgggaggggg agaagtgctg gtagtgcttc caccagtatc
     4081 cccaggcagg attggtcatg ccgagttcca ttccatggtt cttcgcataa ccggcagaga
     4141 gtttgtagac tccggaggag atgtacacac acgcaaaatc cgcctgcaaa acttgtaatg
     4201 cgagagaccg cagctctggc gcataatgga gggtatattc cagaactgtg atcgccaatc
     4261 ccccccagta gagaatgtag ccgggagcac ccattccccg cagtaacccc ttccagcgca
     4321 tccctacaaa gaaatacctg cagagcacga gattgagtag agctggccat ggcgtccacc
     4381 agccaccggt gatacaaacg ccgcaggcaa accacaccgc cattacgagc ggaaacacaa
     4441 gcgggttctg tatgaactca acctctcgta gtgactgagc ataccctccc cagcgctcgc
     4501 tcaagaaaaa gcgccgccag ttagggatcg ccaacaacaa catgccgcac agcaacatcc
     4561 catacacgac acgcatgaac atctgcacgg agaggctcag ctccgggtgc aaaaaaccct
     4621 gtggcgcgaa aaacatctcc atatagcggt caaccgtttc ctaccggaag gtatagccca
     4681 actgaactca cgatgttatg cgcaccacgc agactaatga ggcaccacat caacgtgatc
     4741 cgaggaagga tctttgggaa ggcgagctgg cagcgtacaa gcatcggact gacacccaaa
     4801 cttcagtcga tttttcgcca cccacgcata gaggcgagtt cccaccgggg tagcgccagg
     4861 aaaatagaaa accgggatca acggccacaa taacggacta ctccatatca tccggcgaaa
     4921 cgcaaaaaat cccctataca cttcgcgtcg ctcggtcacg acaaacatgg cattgtcaaa
     4981 gtcggcccct tgtaactcag gaaacaacgc agcgaccgac tgttcgtcat gggcatcata
     5041 aaaccgaaag accttgaaaa tatcggctgc gctgaacagc ttgaggctac gaatacaaaa
     5101 gcggcattgt ccgtcataga tgacatacac ccgctccttc atactcatcg cgatcgcgac
     5161 tccttcatca ctcacgcgcc caaacaagtc cccgcctgca tgattcgttt caggtccaaa
     5221 ccggtcatgc gttccccatc cagctcagcc gtctcaataa gcacgtcacg atctcgagtg
     5281 cacacccgag ctccctgcgc gtcaacttgc acgacaagcc ccggcacccc actggtctct
     5341 tgtacaagga ggcgacattt ccatattact aattttttct tgttcaagat agtaaaggcc
     5401 ccgggccacg ggcgaaccag agcccgcaca aggttgcaga cctccgtcgc tgatgcatgc
     5461 caattgatca agccatcgtc gatcgtgcga agccgatgat acttggcggt cgcctcatcc
     5521 tgcttttccc tgggggctcg tccttgactg atggcaggcc accattcctt cagcaaggcc
     5581 atcccggcct ccttcaactt ctcccgcacc gtggctgcat cgtcatctcg ctcaattgga
     5641 aagcgtctct gtgcaatgac cggccccgta tcaattcctt catccatata gtgcaaagcg
     5701 acacctgtct ccgattctcc attaatgatg gcccactgca tgacatgccc cccgcgatac
     5761 tcaggcaaca ggccaccatg cacattgacc gttccctctc gtggcacctc aatcacatca
     5821 ggggggagga tcatcgggta tgaccacacc acaatgacat caggatcaag ccgctttaag
     5881 tcttggacga atggcgcaat cgtcttcctt ggtggctgca cgagtactgg aattcccgcc
     5941 gtgcaagcaa agtctttgat aaagtcgaca tcgtcgccgg atcggttcga gggtgccacc
     6001 accgcgatta cctccgcccc agccgagagt aacgccgtaa cagcggcggc agctaacgcg
     6061 ccgtacccga agacaatcac gcgcccacgg tgcgcctcag ccttattctg aacgacgttc
     6121 ttctcagaca ttgactcact caacgtgccg ggcctcaccc tagactttcg gttgaaagac
     6181 ctcaacttct acgcctaagt cccttcgcat cacttccttc aaatggtccc ctgaattccg
     6241 gaggaccttc tgccacccat gatcccaatc acgaatacca gcgaaccagg ccgaacaccc
     6301 acggcagggg aaacattccg ccccgcggcc ctcgagatgc atcctgcgat accacgtgaa
     6361 catttcaccc tgccaaattt ccttaatgct ggtgtccttc acgttgggaa agagctctga
     6421 ggttttgaaa gaaatatcct gcccacagag agcaatccgc cctaaggtat ccacattcaa
     6481 tcgctcaaac ggccatacgc acggttcttt tttctgtttc ggaaggtccg cgtataaatg
     6541 ccggtcgaga gttttcccaa gctggatcgt cgtattgtca tcccaagaaa gaaactttct
     6601 ggtaatgacc tcatccacgc cgacatcctt gagccaaaac tgaacagcat catccagtct
     6661 gccctcgatc gcctcttgcc gaatgatgga ggtgaccact cgggtcgtcg ccacatgctg
     6721 cttcctcaaa tccaacgcgg cgcgaacatt gccgacatgc ttcgtccacc attgctccgg
     6781 atccccagtc ttcccacttc gcggtggacg taagaccgaa taggtttcgg catccgccgc
     6841 atccatcgaa aattcaatga gatcgattcc ggctcggatc acgcgttcca accgctcccg
     6901 atgcttggca tttggaccaa acatactgcc gttggtattc aaccaaaccc tggccccttt
     6961 cgccttggca tattcaatca tctcgaccat tttcgggtgg agcatgggtt cgccaccacc
     7021 ggtacaccgc atccatgcgc cataagggcc acactcatca gcaattttct cccatagttt
     7081 caccggaatt aaatcgccgt catgttcgtg gtagaaccgg cgcaattctg aattggtgtc
     7141 ggtataggga cagtttgggc accccatgtt gcacgggtaa atgatggaga taatcaccat
     7201 caagggaaac tcttccgcgc caggctttag cccatatgtt ttctcgtctg gaacgaccac
     7261 cagcggcttg cttgtcacga tgtatacccc caattagcgg cagttctaga aataatgttc
     7321 tcacagtcgc gaacgtcggc gactatcgca cgaatcaaca cccatagaca catctggctt
     7381 gaccctgaat tttgcaaatc ctagctgaat gcttgaataa cggaacagcc cgtgcgagca
     7441 ccaaaccagg aaaggtgaag agattagtat ggcctactct ctcattctgc catcatagtt
     7501 ctcaatccgt ttctatacta caacccggca gtccacattt tccccgcccc aaccctgtat
     7561 gcactcagct gttaatcagc aatcctcttt acgtgacctt gataccaacg atgcgcatct
     7621 tgcaagccct ctctgagcgg aatccgtggt gtccatccca atgccttcat gcgaccgcaa
     7681 tccatgagct ttcgtggcat gccatcgggc ttggtcggat cccaatggat atcgcctcga
     7741 tagccgacga cctcggccac taaagtggcc aaccggccga tcgaaattcc ttcgccggcc
     7801 ccaacgttca cgatctctgc atgggagtac cgctccatca gaaacaggca ggcgtccgcg
     7861 caatcatccg catgcataaa ctcgcgcaat ggcgtgccac tcccccataa cgtgggttcc
     7921 ccgccctgtt cccgcgcttc gttaaatcgg cggagcaatg cggccaacac atgggaatgc
     7981 tgcaaatcaa agttatcgtt cggcccgtac agattcgtcg gcacagcgac aatgaagtcg
     8041 cagccgtatt gctgccgata ggcctggcac atcttgatcc ccgcaatttt ggccactgca
     8101 tacccttcgt tggtcggctc cagaggacca gttagcagat agtcctcctt cattggctga
     8161 ggcgagtcac gggggtagat acaggatgac cccagcagaa gcagtttctt cacgccatga
     8221 acataggctt gatgaacgac gttggtttga attgccaggt tgtcgtaaat gaactctgca
     8281 ggcaacgtgc tgttggcgag gattccaccg accttggctg ccgcgaggaa cacatagtca
     8341 ggcttgacct cttcaaaaaa tgcggccacc cgcacattgt cgcgcagatc caattcccca
     8401 ctggcgcgga cgatcagtcg ctcgtaccca cggcttgtca gagcccgcag gattgctgac
     8461 ccgaccatac cctggtgtcc agccacgaaa atacgtgcct gcttttccat cgactacccc
     8521 tcagtgcggg ctttgcaacc gcagccgctc gactaccgat taatttttat gtcgtacatg
     8581 gaatcaggca cctacatcgc tatcctgtga atcggcaact cggtagattc ctcggtagat
     8641 tccccggcag aacgatttcg tgtgtagctg ggcctatggg aactttgcct ctaaaatact
     8701 tccgatcagc ttgggtatct ggcgaaaaat tcccacattg ctgagtggac gaatgaggag
     8761 acgtacccgc atcgctcgcc atataactgt gagcaatggt cttgacgctg gaccactgtt
     8821 gaccaagccg atattgaaaa ttttctacga acgatggcaa caattcaggt ttgcagcagg
     8881 aacacaccca gagcacgagg gaaccttctc gctagcagta tagagaaaag agcgaggttg
     8941 cacaagaaat gtgccgggcc aggaaggcgg ccacctaagc tgatgagacg gttcagagaa
     9001 gggcgcagcc tatgtgcggg aggacattga ggacagggcc ggcagagctt tcaggaatcg
     9061 ctgcaacccg ttctgccagg acggcatgct gaacccgagg tgatggagac ggtctgagga
     9121 cagcaccgaa taggccggtc gctttgccgg tcttggcata tcctccgtag tgattggctg
     9181 gacgggcacc cggtagccag ccagtttaac aatctccgct gcaaattcat gccaggtaca
     9241 gtgaccttcg ttcgtgacat gcaaaaaacc tcggatcgga tgcgtcacga gtcgatcgat
     9301 catccgagca agatcttctg caaaggtcgg gcagccctcc tgatcggcaa cgacccgaag
     9361 agaaggacgc tcggccgcca gctgaagaat cgtcttcacg aaattcttcc cctgcaagcc
     9421 atacagccac gcggtccgca ccaccaacgt attctcgcag caagccagcg cccgcgcctc
     9481 cccggcgcat ttggacgccc cgtaggcgct gaccggattg gtctgatcgg tttcaacgta
     9541 gggacgtgtg ccacggccat caaagacgta gtcggtcgag atatatatga gcctggctcc
     9601 cacctgagca gccgcgcgtg cgacgcgttc ggtcccgtcg gcattgatcg ccatggccaa
     9661 ttgcggattg cgttcggcgc catccacatc cgtatgcgcg ccggcgtgta tcaccgcgtc
     9721 gggtacggca tcgacaatca ggcgaccgca gtcctcatgc gtcaggtcaa acgtgggaag
     9781 atccagtagg gtcagctgat gcccctgcaa cacctgctga agctccttgc ctaattgtcc
     9841 ctgggctccg gttatgacga tccgcacgcg gtccccttgg ccaggcgctg gccgtactgt
     9901 tgctgataat atgccttgaa ctcgccggac ttgatctttc gccaccaatc ctcatgcgtc
     9961 cgataccact cgaccgtctg ctgtagtcct tcctcgaacg gcacttgcgg cgaccagccc
    10021 agggcgcgca accggctgca atcaattgaa tagcggcggt cgtgtcctgg ccggtcctga
    10081 acaaagcgga tcagtgatcg cgactttccg agtgccgcca cgatctgttc ggcaaccgtg
    10141 atattttcgc gttcgttccc gccgccgaca ttgtagacag ttcccggctg gccatcttcg
    10201 aacacgtgct ggatccccgc ggcatgatcg tagaccgaca accaatcccg gcattgtttc
    10261 ccatcgccat agagcgggag tggttctccg tcgatggcat tggtggcaaa caggggaatg
    10321 aatttttctg gatactgatt cggtccgtag gtattgctgc cacgggtgac gacgacagga
    10381 aatcggtagg tggtccagta gctcaagacg agcagatccc cgccggcctt gctggcagaa
    10441 taggggctgc gcggttccaa ccggtcccct tccgttgaac tcccctgttc gacgctgccg
    10501 tacacttcat cggtgctcac ttgcagaaat cgctgcaccc ctgcctgccg cgcctcctcc
    10561 agcagaatgc cggtccccac cacatcggtg cgggcaaagg cccccgggtc aagaatcgat
    10621 cgatcgacat gcgtttctgc cgcgcaattg atgatgccct caatccggtg ttcctgcagc
    10681 gcggcatgca cagctttctg atcaccgata tccgcatgaa tgaaacgata ctgaggatgg
    10741 tccacgagat ccgtcaggtt ctccagattc ccggagtatt tcaaggcatc aagattcacg
    10801 accgaatgac ggccgctctg cactaatcga cgcaccagat gcgacccgat gaacccggcg
    10861 ccacccgtca cgagaattcg catcagaact ccatcctatt cgcaccggtc tgcgccacga
    10921 gttggttggc ggcaagcagt gactcaattg tgccggcatc cgtccaccag ccgtcgagca
    10981 cgttccaggt caactcgccg gaggcgatat acgcattgtt gacgtcggta atttccagct
    11041 caccgcgccc tgatggtttc agcgtcctgg taatctcgaa tacccgggca tcgtagaaat
    11101 agatgccggt caccgcataa gaggacttgg gatgagccgg cttctcttcg atactcagaa
    11161 cctggttgcc atcgaggtga ggcacaccga aacgctgcgg atctttcact tccttgagca
    11221 aaatctttgc gcccttcttc tgggtcctga acgcctccgc ggccttcgcg atgttgccct
    11281 gaatcaaatt gtctccgagc acgacgcaga tcggaccatc gtcggcaaaa tgttccgcca
    11341 ggcgcaaggc atcggcgatg cctccttcgc cctgctgata ggtatagctg agatgtttga
    11401 gaccgaagtc ccgcccattg cccaacaact tcaaaaaatc accggcactg tttccgccgg
    11461 ttaccagcat gatttccgtg attccggcat tcacaagcgt ctggatggga taatagatca
    11521 tcggcttgtt atagaccggc agcagatgtt tgttcgtcac cttggtgagc gggagcaggc
    11581 gtgatccgag ccctccggca agcacgactc ctttcatccg tcaccccccc cgcaacagga
    11641 tcttcagaac accacagccg tcagcacgag ctgccgcctg cgaacctagc cttccttcat
    11701 gtcgatcgta acgtctcgcc cccagttcag actagaaaat tcgtcgatat cgtaggcgcc
    11761 ggtcttaccg gccaaccgct cgttcataca ccgcgaccgt ttgtttcgct gtccgttccc
    11821 acacaaattc ttttgagcgg gcgagagcac gctcacgcaa ggacagctgc aattcaatcg
    11881 attccaaaac cctggccagt tcctgcgtta attggtccgt ccggcgcgga tcgacgagga
    11941 ccgccgcctc accggccact tccggtagcg atgacgcatt ggagcagatc accggacagc
    12001 cacagcccat cgcttctagt accggcagac cgaatccttc atagagtgag ggaaacacga
    12061 ataccgtagc ctgctggtaa taggtcacga ggtctgcatc ggacaggagg ccgaccagtt
    12121 tgacctgagc gccgagccca cgccgagcaa tagcggcacg aatatcctct cgtttgcaca
    12181 catcgcccgc caagatcaga tcgaaccggt tgcgcaaatc ggcgggaagc gtcgcgaagg
    12241 cctcaacgag cacgccgaca ttcttggtcg ggtctgatcc ggcgacgcag aagacgtatg
    12301 gcctgccctc atgccgatgt tcggccggcc ggaaccgccc cgcgtcaaga ccaagcggtg
    12361 tcacactcac ctggtccggt cgaagcggca catgcgccag caaatcacgc cgggaatgct
    12421 ccgaaaccgt gatcacctgg tcgatcgtct gccaacgatc ccctacaagc cagtcttcga
    12481 tacgactgtt gaggttggtg cggggacgga gttcaggaaa cagcagcggt ttgacatcat
    12541 gaatggtcgt cacgaacttt ccgcgcttcc aggcgacgca ggaatcgtac ggaaaatgca
    12601 gcacatcgta cggaccaacc atcagcggcg caacctgctc ccagaatcga cggcgaaaca
    12661 gcggaaaccg caccacgcga tatcgcatat tcggccgcgc cgtgaaagga tgttccggct
    12721 gaggaaggag gatgtcatag tgattcgtct gatccacgct ccccagcgcc tcgatcaatt
    12781 cccgggcata ccgcccgatc cccacgttga aatttttcag gtgccaggcg gcgatgacga
    12841 tcttcacaag gttgcgctgg atggacccct ggtggaggag atctgcgtga cgcgacggga
    12901 tggaaccata gcccacccgt caagtcaagg caagccggct ccatcacact gggtggccca
    12961 atggtaccgc ggttgactgg gtttgccgcc tgtgttaccg gtgtgacggc acaaggaatt
    13021 ttatccgtgg cacacgtcag cgtcgtcatc cccgcataca atggtacgtc caggtacttg
    13081 aacgaggcca tccgttccgt tcaggcacaa agttttgcgg actacgagat catcgtcgtg
    13141 gatgacgcgt cgaccgatcg cacggatgcg ctggttcagg ctattccaca ggttcgatac
    13201 gtacgccact ccaagaacgc cggacaagcc gccgccagaa ataccggcgc gcaactggcg
    13261 caggctccct atctggcgtt tctcgatcag gacgatctct gggaaccgac gtttttagaa
    13321 aggaccctgg cgatccttga accggccaca ggcgcggtgc tcgtccattg cgacggatac
    13381 caggttaatg aacgaaacga tattctcgag tacgacgcag cgatgaaaca tcagcggagc
    13441 attacgcaat tgcttcgcgg cggccacgat gccgccacat ccggctcatt gttccgcaaa
    13501 tcctggttcg actcggtggg aggctacgat gcagccctct cgatctggga ggacatcgat
    13561 ctggccattc gactctatgg accagaacgc ttcatccacc tccctgaacc gatgtaccgt
    13621 catcggctgt atggccacaa tgcgtcgaga gacatcccct ctctgcgcgc actggacggc
    13681 cgccggcgat ttcttgagaa acatgccgcc acctgccggc ccggcacacc ggaaggcgaa
    13741 gccctgtcga acgactgggc catttattat gccgacctcg gcaagcatca cctgcgggcg
    13801 catgcccggc aagaggcgcg acaggcattt cgattcgccc tgcgtcacca accgctcaat
    13861 ttcaaaacct ggtcccgact cctcagatcg tactttgtgt agcctgattc aggctcgcat
    13921 ctttcctcgc gtagactttc agttcagtcc catatcccag atgtccgaca caaaaacaaa
    13981 acgggcggat cagcgatttt tcaaggagac gacggcaggg tattgccctc ccgaatcgtt
    14041 gctcacacac tcggtccagg ctagccatga agtagagcga acccaggccg gtgcgcatcg
    14101 attcgatccg gaatccggcc gcttccaaga tccgccgcaa cgtcgccttt tcataatgaa
    14161 agagatggcg gggcaactcc caaggaaacc accagcgacc aaagatcttg gcctccagac
    14221 tgccggcatt cgggcagaga atgacgatcc ggccatcctg atccaaaatg cgccagacct
    14281 ctttcaggag cggcagcgga ccgtgcagat gttccagcac atgactgaac aggaccgtat
    14341 cgaagctccg gtccggatag gctatcgcct ctaaatcacc cagacgtacc cgatccccga
    14401 agcaagcctt cgcctgtgtg acggccacgg cgctcgcgtc cacccctgat acctcccaac
    14461 cctgctcctg cagcgaggcg agatttcccc ctgccccgca gccgacatcc aacacacggc
    14521 cttgccccac ccacggcaac aggccacgcc cgcgccagca gcgccacaag aactcaggcc
    14581 agaggaacat tcgccgtacc cgttgccatg ctcgcccctg ctcctgtgcc ggatagccat
    14641 aaaaatcctg ggcgatccat cgcctgattg ttgccgacca ccgtttggca gcacggcgga
    14701 gatctccggc ctggcggggc gccgcatcag ggtaatacgt atcgggataa tatctgccga
    14761 tttcttgccg cgtcggacga ggattcaaga acaattgacg gcaatccagg cagcgcacca
    14821 cggtgaacac atcatcggtg acgcggtggg tgagatcgta ttggctgaac atcgtcgtgg
    14881 tcgacgtccc gccgcacact tcgcaatcga tcgtctccat cacctgttcc acatgagggt
    14941 tgcctgggca gttaagacgt ccaggtgcgt agccagcgcg tgtaacaacg tatccggccg
    15001 ggagccttgc cgattgcctt tcgatactgg ctccgcgcct cagccgggcg accggctcgt
    15061 tctgcggccg cgcctttcaa cagatagtaa ttggcccatt cccgatccag tgcagcccgc
    15121 tgcgcagtcg tcaacggcgc aaacccttcg agacgcatga gaaagcgatc ccggctcgcc
    15181 agtacccgct cccggtgcgt ctgttcatcg aaggccatgt cccgataccc atgccctcgc
    15241 tgcagtttcg tcaccaacac ctgatcgata cactcgaagc tggtcacccg gaagagcctg
    15301 atcgtgagat cggaatcttc cgatccgtag agttccggat ccaagcgtcc aacttgatcc
    15361 agcacgctct tccggatcaa cagggtcgag ggcaatgcca catgtccccc ggcgaacgcc
    15421 tgcaccatcg gctcgcggct cgaacgggcg atgacacgtt cttccaacgt cccgtcttcg
    15481 aagcgcacgt caaaatccgt gtggaccaat cccacctccg gacgggtgcg catgatcgcc
    15541 agctgcgcgg ccagctttcc cggcagccat acatcgtcgc agtccagcag ggcgatgtac
    15601 tgcccgcgcg ccgcttcaat accaaaattg cgagaccggg ccggcccccc gtggctccgt
    15661 tgaaaccacc ggatgccgtc actctcctcc aggcgcttca ggacttcttg tgtgccatcc
    15721 gtcgatccgt catccacgac gagaatctcc acgtcaccat gcgcttgtgc ccgcacactg
    15781 gccacagcct tggcaacaaa tggcgccccg ttgaaaacgg gaatgacgac actgaccgac
    15841 tcattcatgt cacaaccctt gcacccttca gatccaggat ccggcccgcc gcttcgaata
    15901 cttcatcgac cgtgatctgg cgcatgcaac tatcgtctcc gcgcaagcag ctgggatggt
    15961 aacaggcccg gcaatccaga cctttgtaga tcacctgcgt caatccgcca cgcggccccc
    16021 atcgctcggg ataggtggga ccgaacaagg ccaccaccgg cgtgcccacc gtcgcggcaa
    16081 tatgcatggc accgccgtcg ttaccgacga acaacgcgca ccgcttcacc agtgaggcat
    16141 actgcagcag ggaaaacttt cccgctacca cgataggagc gcaatgcgac tttcgggcaa
    16201 cctgttcggc gacctcacgc tcccgttggt ccccccccag caaaatccgg caacgaaact
    16261 gttcaacgag gcgatcgctt aattgcgcaa accgttcagg cggccagact ttcaacgaat
    16321 accgcgcccc cggttgcagg agcaccagcg gcgggcattg gtcaccgatc aatccctgct
    16381 ccgtcagcca ttggtccgtc acctgctctt ccgagtcgga cacagagacc gacggcatac
    16441 cccgtttggg atcgagaccc aacgctctga gagcgcacag atcatactcc acgcgatgct
    16501 gatcgacggg gcgcggcttc gccaccctgc tgtacaagag tccacgccag cgatgttccg
    16561 cattaaatcc gatccggatg ggtgcgccgg tcgcgaggct gatgacggca gatcggtccc
    16621 catcggtcag gtcgatcaca caatcgaatc cccgctctcg gagacgatag acgaacttgc
    16681 attggtcggc ccatgcgccc ttttcaagac agagcacctc gttcacatcc ggattatgcg
    16741 caagcacccc ttccgtcccg cgattgacca acatcgtcag ctgcgcggcg ggaaaagcct
    16801 ctctcaaggc gcgcaacacc ggcgtggaca acagcacatc cccgatatgc cgcaatttga
    16861 tgatcaggat gttcttcggg cgcagcaggc cagtagctat catgtgcaga gctcacgata
    16921 gagagcactg gtggaaactg ccgcgcgctg ccaggtgaag agcctcgccc gttcgtatcc
    16981 gcgctgtcgc agattctctc tgagatgcgt atcggacagg acggtcacca tggcatgggc
    17041 caattcttcc acattgttcg gatcaaccag caatgctgcg tcaccagcca cctccggtaa
    17101 cgatgaggtc gaagatgtca cggtaggcgc accacaggcc atggcctcca acaccggcat
    17161 gccaaatccc tcataatggg aagggaacac gaacaagtct gcacaggagt acaatcgacg
    17221 caagtcttca cggtcgagac gtcccgtgca acgaacctcc tgctccaagc cgagcgactg
    17281 cgccgtcgct gtataactgc cctggggatg ctccgcgtct ccgaccagca cgatggctcg
    17341 tccgcccagc tgtgagcggc acgcgcccac cgcctgtaaa aatcccacgt ggttttttct
    17401 cggatcagcc ccaccgacga agagaatgaa gccggacgag ggaatggacc atcgtgtccg
    17461 tacctccgct cgaatctggt cgtcacacag tggcgtgaag tcctccgaca cgccgttata
    17521 aatcaccctc acatggtctc gtggaatgcc atacagcgat tcgatttcgc gggccgaaaa
    17581 ctcggagacg gtcacaacct tgcgcgcccg cttcgccgtc gcaatcgcac ggcgggatga
    17641 ccccgcctgc ccgaatagtt tgcgtgaata ttccggatgc cgggccagcc acaggtcatg
    17701 gatggtgaca attccaccgt accggcccgt cgtgtgcatt ttaaaattgg ggccgtgata
    17761 caggtcaaga cgatcccagc ggcctcgcca ggacatcaac caccgtggcg tctccatcca
    17821 ctgcatgcgc gagcgtgatg gccaaccgct gagttgaccg gcttgcagcg atccggggcg
    17881 gagataaccg acgtagtccg tcttctcatc cagttgcaac agcgccttca gcagatgata
    17941 cgtatgccag ccaacgccgc ctttatcgcc caccaatgag gacgcgtcga tcccgatacg
    18001 catcagagga acgagacctc agtcccaggc cggcgatggg gcaccgagac catcatgcgt
    18061 ctcgcctgac gttcaaccgc tcccacgcct tggcatattt cacgaaggtg tggagaccgg
    18121 cgaacatcgc caccaccagc ccatgcacgc cgtcgcgata tccaccgcgc gtgaacaacg
    18181 tttttagaat agtgccgagg tggtgcgtgg caatggtcca gccgctgata tggcggacac
    18241 gcttcaactt ttcatcggcg cccagcgtgg tataccgcat catcttgcgg atgtggtggt
    18301 tcacggtggg catgctgtaa tgaatgaacg gctcccggag acgctcccgc cggccgctca
    18361 gcgcgaggtt ttcgtggagg cgcacatcgt catagcggcc ggccgtcttg cgaaacagcc
    18421 gcagttgata gtccgggtac aacccgccgc cttgaatcca tttcccgtaa aacacattcc
    18481 ggcgggggat ttcgtagccc ccaatatcag caggaggtcg actgtccacc agtgcttgga
    18541 tttcctgccg caaagcgtcc ggcactcgct cgtcggcatc caccaccagg atccagtccg
    18601 caccggcttg gtcgatccca aagttctttt gcggcccgta ccccggccag ggacgcacaa
    18661 acaccttcga ggtgtacccc tttgcgagct cgacggttcg atcccggctc tccgcatcca
    18721 ccacgatgag ctcatcaacc caccgtagcg attgaagaca ctccgcgatg ttgtgctcct
    18781 cgtccttggt gatcaccact gccgcaatcg tcatgccgtc tcgtcctgct tcacgatact
    18841 gtgcctagcc ttgtttcgcc ggggcaaccc ggttcggctg ccgaacgccc tgggattgca
    18901 gttcccagaa ccgcgcatat ttgatgaatg tgtaataggc gtagaggccg gacaaaatga
    18961 ggccggggac accatccagg catcccttac gcccgacata catcttgagg aaggtaaaac
    19021 acggatgcga cacgagctga tgccacgaaa acgctcggcc ctgttcgacc attcgcttag
    19081 ccatcagatc ggaatagcgc tcctgcttgg ccaggtaatg atcgatgtcc cggaatggat
    19141 actggagcgc ggcggccgtc atgtagccca gtcgtccatc cagatcgaaa ctttcatgga
    19201 cgagttcatc ccgataccgc agcttgctct tccggaacaa ttgcggttga cgatagtcgg
    19261 gataccaccc gcaatgtttg atccaacgcc cgagaaaata atttttgcgc ggcacgaaat
    19321 acgcgtcggc ttccggcccg gcgtccaaca gccgcctgat ttcgtcgcgc agttcgggag
    19381 tggcccgttc gtccgtgtcg agactgaaca cccagtcatg cgtggcatgc gccagggctt
    19441 cgttcctcag acgtccgaac ccatggaagt catgctggta gaccttgtcc gtgaactcgc
    19501 gactgatgcg ttcggtgccg tcggtgctat gagaatccac cacgatgatt tcatccgccc
    19561 aggtaataga ttccaggcag gcccgcatgt tgggttcgtc gttgtaggca atgacgaccg
    19621 ccgataccgg tatacgatcc acggagaagg gtccttttta catcgcgtcc acggtcagct
    19681 tcggccgcag actggtgatc acaatcggct tccgaattcc cgccaggacc acagtcgatt
    19741 ggaatccatc gcgacattcg cgacttcgta taacgccatc aattcccggt cacgggacac
    19801 cgacagccgc ctgccgcctg tttgttcatc cggtaccggt tcgaaccgta acgtggcttc
    19861 ctgaaaggtt tccgtcgcga acgaatacaa caacagtcct tctgcgctcg caagcccgac
    19921 caggttgtcg tacacactcg tcagataggc cacctgacca ggcaggcaat cttgcaccag
    19981 ggcgcagctc acatcatgtc ccgcaaatgc tcccatcgcc ggcagtcccc cgttcaatga
    20041 caatagggtt ggagccacat ccacctggct ggcgatcgta tccacgactc gaggccgata
    20101 ggtcgcaggg gttcgtaagg attcgtccat ccacagaaac aacggcgagg caaaatgccc
    20161 gacctttcgc tcgatatcgg accctcccac cggttcatgc cggccgtgat cacccagaat
    20221 gacgacgatc gtattccgga gcaatccttc gcggacaagc cgggtgaagg tccgctccag
    20281 ttcgagatcc gtgtatcgca aggcggcgac atacttatcc tgcacctgct gttgaagcaa
    20341 ctgaacctca ggatgattca tgggggccgc gaacggatga tgcgtcgaca gcgtcagcgt
    20401 ggagaggaaa aacggcttgc cctgggactg ccgcagcttg atccgttcat aacagaggtc
    20461 gaacagcgca ccatcgacaa tgcccagtcc tgcccgttcc gtattcgcgg ggaagtctcc
    20521 ctcgtccagc aattgttgca agccattgcg ggcgacaaac gtctgcaacc ggttcaggtc
    20581 ccgatgctgc ccgatcacca tttccgtact gtacccttgc cgttgcagga gggacggcag
    20641 gcacagatag tcgtgagcgt atcgcgtctt cattgcggca gcgccttgtc gcggaaaggt
    20701 actgcagagc gtcgcgaaca gccccctgga ggtctgcact ccgttcgaaa aaaagttctg
    20761 gaaatagaca ctgtcctgtt tcagccgatc gagaaagggg gtgcccttca cctcgccata
    20821 ggtctgcccc agatatcggc gatccaatcc ttcgagaaat accaacagga tgttggccgg
    20881 cttggacagt cgaacgccat cgggggaagg cgaagtcgtc cgcacgaacg gataccgcgg
    20941 atccacaaat gtctccccgg gacccagcaa ctgttgagcg gtgcgcacgg ccaggtcata
    21001 aggcatggcc tcggtattcg cccgccgttc gatcggattg cgggatacca cggcaatccg
    21061 caaagcctcg gcggcaaaca gcacggggtt ctgcgccagc gtgtaataca ccatgctgcc
    21121 gatcggagcg atgcgaatgc cgtacggacc ggaatggtga aacccggcgc cgccggccac
    21181 gagtgccacc accaacaacg cattggcttg aaaacctgag cccggccgcc agcggtccag
    21241 cgccggcacc accgcgcggg aaaagctcca ccaccacaat ccaatgcccg cggcttggat
    21301 ggctagaaag accgtcagtc gccaggccca tttcccggca gcttccaact ccgcgctggt
    21361 ctgttgcatg gcctgcacgt tcgtcccctc agtcgtcgcc gcgggcgcga ggagatcgcc
    21421 gatatactcc aggaacacaa agtccatatg ttgatgattg aagccgtagt accccatatc
    21481 gacacacaag agcacgaaca ggagcaatcc ggccagcaga caccccacat ggaaggccgg
    21541 gcgaaacgag agcgaaaagc gccgcggtga ttggcgaaag ccggctaccc cctgccgcaa
    21601 cagggagtag agaccggccc cgactccgcc cagcacgagg gcgaaggtgg cggtgatgaa
    21661 atctcctcgc acaccgacga ggagtgtttt gagcagcaac gacgccgtgg gcgtctcctg
    21721 gtcgagcgca tcgcgcagca gaaacacccg ctccgccatt tggatgacga cgaacagaat
    21781 ccaccagaac agcacgcttt tgcggataga cgctcccgag tcggcccatc gtggcatcat
    21841 gacgcgatga ccttccgata gacccgctcc aattcatcca ccatccggtc gatcgaatag
    21901 gactgctcca cggtatgacg cccgcgtttc cccattgccg cacgcagatt ggcatccttc
    21961 aggagacgaa cgatgcgatc ggccagcgct tcgggatcgc gtggtgggac gagaaaaccc
    22021 gactctccgt cggcaatcac atcgggaatc gaaccggtgg ttgtcgacac gaccgggaga
    22081 ccgaccgcga gagcctgcat caacacttgt ggaatcgtat ccccctcgac cgtcgggatc
    22141 acaaataggt tcaacgaccg gaagacgtcc aggagatcgt cacggaatcc caacatgcgc
    22201 acatcggctg ccagcccgag gcgttcgata tgagcacgaa tgttctgctc ctccggcccc
    22261 tcccccacaa tgaggaatgc cgccgccgga tgttgtttca acaccttggc tgccgcgtcg
    22321 accaggtagc gatgaccttt atagtcccgc aggtaggaaa tcatcccgac cagcaaatgc
    22381 cccggtggaa taccgagctc cgcgcggata tcgtgcgtgg gttgagccgg agaaaactgt
    22441 tccacatcca agccgatcgg gaacgccgcc acacgatccg gcgacaagcc gtctcgatgt
    22501 atcaggtcct ggcggttccg ttctccgccg gtgacgatca ccatgtcgaa caggcggcgg
    22561 tataacaggt gcgtggtggc attgttgttc agcggcgctg agatatggcg cgtgcgaatg
    22621 accttggggc gaggactgag gagccgcgtg gagagggctg cgatccagct gtcccgcgaa
    22681 ctgttgacgt gaacgatgtc gggccgctca cgcgtgagca actgacggaa cgcaagaatg
    22741 gacagcggca ggcgccattg ccgcatcacg aacggctcga ccgggagccc acgctcctga
    22801 gccatcgcca tgatgggact gcgcggctcc aacaccaggc ggatgtgatg gcctcgcgcc
    22861 accaatcgtt cggcatgcag gaggacagcc aactcctgac cgccgaccgc catactggat
    22921 tccgcaatga gaatgttcac gatgatcctg atgtatccaa tcgccccaat tgccgctgca
    22981 ccgcgcgcac gacttgctcc ggtgtcacgc cggtcaaaca ttcgagcgtg gcggcgcggg
    23041 agcactcacg acgaaaacag gggcggcact cgaccgggtt ggacaagacg acatgcccct
    23101 ggccataggg gccggtcttc accgggtcgg tcggcccgaa cagcgccacc accggggttc
    23161 ccaccgcggc ggcaatatgc atcggccctg aatcgttcgt cacgaacaca gctgcgcggc
    23221 gcaacaaact gggcagcaag cctacaggtg tctgtcctgt cagatcaacc gctttcgtgc
    23281 gcatgcgcgc catcaccgca cgcgtctcgg agcgctcggc cgggcctccc ataaagataa
    23341 cgggtatgtg ctgtgcctcg gccaaaagat ccgccgcttc ggcaaagtgt tgcgcgggcc
    23401 agcgcttggt ctcccagcgg gcggacacgt tcatcgcgat ccatggcgct gacaccctga
    23461 tacccgctgc ggtgagcagc gcttccgcgg caccccggtc ttcagcccga tcagcgaagt
    23521 caaaccgcgg gtctgtctga cgcgccgcac ccaaggcctc cgccaccaac agataccggt
    23581 cgacggcatg catcgatccg ttggggacgg cgacgcgctg ggtatacaag aagggactcc
    23641 cttcgcgcgc attggcaaat ccgacacgcc ggctgcagcc ggtcagccag gccatggcgc
    23701 cgcttctgaa caatccttgg agatcgacga ccagctcgaa ccgggctgcc cgaagatccg
    23761 gcacgcgccc caaccaccct ctcaacccct gctccaccgc gcagacatga tcgactcctt
    23821 tgatgacctc gacgaaggaa gcccattggc gcttgaccag ccacgtcacc tcggcctgag
    23881 gaaatcgctc gcgcaaggcg gccagggtcg gcatcgcgtg cacgatgtcg ccaagcgaac
    23941 tcggtttgat gagtaggatg cggcggactg gctcggtcat tcggccacca gggatgatgc
    24001 tcgctcagcc tgtcacatcg gcaaccggct tgcgcgtcgc gtgggcaatg gtccattcaa
    24061 ccgcatgcga caggtcagcc gccacatggt ccggagaaag atcccgtgcc gtcagatccg
    24121 tcagcgcctc ggcccctgac gggccggtca tgaccagcac agcccgcgca ccaacctgtt
    24181 tggccaattc gacatcacgc acactgtcgc cgactacata ggcgcgactc aggtccacct
    24241 gcaattcggc taaggcgcga tcgaccatcc ctcgagacgg cttccggcaa ttacagcgat
    24301 cgtcgggatg atggggacag aagtacaacc cgtcgagcgt gaccccgtct tcagccagca
    24361 ggagtcgcaa tttgttatgg atcgcttcca gatcctttga gctgaaatac cctcgcccca
    24421 ctccggattg attcgtcacg acgaccagct ttgcgccgcc gcctttcagt ctggccagtg
    24481 ccgcgccgac accggggagc agcgtaaatt cttccggcgt cttcacatac cctgtatcga
    24541 cattcaacgt cccgtcgcga tccaaaaata cggtcactcc cgccagcgcc gtgggtgaca
    24601 aggcaggtgt caccactgcc ggcgcactga ggaagggagg ggccgcgagc ggcagatgtc
    24661 gtgctgctgc atcgtggacc tgctccaccg tcactccggt catacagcga tgatcgatgg
    24721 gacattcccg caacaggcat ggtgcgcaag ataccggctg ccg
//
LOCUS       DAOQEW010000032        24682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_479_length_24682_cov_7.458156, whole genome shotgun sequence.
ACCESSION   DAOQEW010000032 DAOQEW010000000
VERSION     DAOQEW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_24682_cov_7.458156"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLS35_05485"
     CDS             complement(<1..995)
                     /locus_tag="PLS35_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18141.1"
                     /translation="MARCPARSAKRLRPDVNGLGLYVLSGALLVLSLGFFPSLSSATD
                     LELIGGPGGSLFRAECPQGSYLVGLAGRTGEWVDRISPICAPWLRGSQAFGLPAVGPS
                     FGASGGGQDRQRTCWGTGNKTLAIQSWYVETVRSPNPPVQYVEIHCASLGPAQSIGLF
                     DFGNPTAASEERVTKGPYPHGCPPGEIASGFRVRAGLFIDSLGLICAPIPARLGAPAG
                     KLATPLVQTPPPSVPFNPQAKKLQVPDDLFVITKPVPGERVTQGQLFIAAIPPAVGVT
                     NVAELELRYLDAPPGQKDSYPFVSIMAVNTTKLTQGYLVAPIVTDAYSGRWQVRA"
     gene            complement(1007..1690)
                     /locus_tag="PLS35_05490"
     CDS             complement(1007..1690)
                     /locus_tag="PLS35_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18142.1"
                     /translation="MNWSLIAGIGFSLTVLSPLEAHAKGDLVAPNDPTCYGSEPKNAT
                     FVATRYTYIFSGSCNLAQTRLAIRVSVPWTGVGTFDPTTGQAAEDILVPAPKISEPSR
                     PYGRFKTTMHCSSDPWLNPTVQCDQIVPTVDAPLDRTAPNAQGYRPEPLAPNIVDIVR
                     ARRRPFTSFMNQDAVNSLNRRFGAYQAQNKTNQVIRQFKKVPGILGRGIEQGDQPSQE
                     TPGTGPSSK"
     gene            complement(1722..2900)
                     /locus_tag="PLS35_05495"
     CDS             complement(1722..2900)
                     /locus_tag="PLS35_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18143.1"
                     /translation="MTPAPARNMSLTWIVGVLGIVVLHCALAVSTEVAVADEPAVVGQ
                     LSMTCSVNTINGTHKPLSSDYAITGQCLLFETSSRGGTAQVNNVAVHWTVQASHQPNT
                     KATFETMNLALSEFGKTTATGTVSSTMQCGSDPWRIMPNGPCRLVTRKTSPPSFPGYV
                     SMAYQQLLQEAPNLPLSFKLNPAQRAAIDKQYHIAMAAQQTFDPSQTTLSKPQGPGVA
                     PFTPGGPNTSDAILSRQMPRILSPASGATVVVGQLLVKIQPPLIGKEPVAELEFAWLD
                     TPAGQPRFVNTIPAMDTAKLLQGQYIDPKVTRLNYGRWEMRVRMIGQGAVAGPWSAPA
                     SFTLVPNQQNQSIQTPSPASPLLKSPSERFGAGTSVIRPRGLNREKSEPSDSAATPAE
                     "
     gene            complement(2941..4044)
                     /locus_tag="PLS35_05500"
     CDS             complement(2941..4044)
                     /locus_tag="PLS35_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18144.1"
                     /translation="MNIWHRHAMTVVAGLVLIVALASMCFGFGFNGVGSAPCSSATQQ
                     MVGSFSSTKAQYVSTGTCTTPGSLGAPRTFPYTVKGAYANNIAEEVIEIAAAPISQPS
                     HPYGTWRTTYSCPSDPWLTMDGPPFGPESLRVKCQIVNRMDNSPSEAYPRKYQDGKPL
                     PTLSDLFNAWRAYKPMSAWVLMPAERTALAAKRDADLKAEAAALAKAQAEKRLRSATQ
                     TLTPYRMSLSPIITAPTAGQRFFNQSPISIKLAPPPPWADTEVRLDGTPVKTAKSVTG
                     YMVRLERKDPNGIWVPHATLPVGAAQAESSTGYTGFGAGAPPAFLSIPGAWRLSAQVT
                     TPQQSGWSDWVEFGVMAPPTNKILQPATKSFAK"
     gene            complement(4084..5109)
                     /locus_tag="PLS35_05505"
     CDS             complement(4084..5109)
                     /locus_tag="PLS35_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18145.1"
                     /translation="MQRHMWGVLLFVLSAIAGDASAAGVVVRIYPMTTVSCHLDSIQA
                     KFTAAKSTYRMTGRCDQQLKYEDHVEVIPIPWTSEGAYAPGQGKVAETIQLNGNPPLR
                     GKIETRLGCVEDPWLVMGKAACTQPVFSSTGDVQSLDYIVTYLRNGFEQTLKPNTTGF
                     SYDRAALVAQRPRDLQAEQQALAETARLAQEAARKEAQQLQKKVQPGSTLLENLAPFV
                     VSPTAGSHYLGMTTVPIKLAPPPGRVTVTLYTVKIERKNPGGTWALVTALPIGLAQAS
                     SPSGYLGWGAGGPDGRSLSLMASPGTYRITAQVSAPQQTGWSQPAEFVVMNTNKAIQR
                     APKTFGP"
     gene            complement(5162..6799)
                     /gene="lnt"
                     /locus_tag="PLS35_05510"
     CDS             complement(5162..6799)
                     /gene="lnt"
                     /locus_tag="PLS35_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNI18146.1"
                     /translation="MTAHQRLRVTDGITPIALALLSAALLVVSLPSPDIGWLGWGALV
                     PILLAIQGLSPGRAAALGLLTGVLASFGMYGWLFEVPSFDLRHAVLLALYVGAYPAIW
                     ASATSWALRRDIPLLLCAPVLWLLIDYLRGHAGFLALPWGTLAQTQHRNLALLQTASM
                     IGEHGVTFLVALGNAALAALCLRRERTYATVALLILVTAHAWGATELFSPASGRSIRV
                     AALQPNIQVGDRTTEAGREANLARLERLTREAASARPDLIVWPESAIPGDVSDPALLH
                     RLQQLSSDTAVPLILGAAEVEKFATGDNLLTIGRRLFNTAHLFQPSGATSPPYRKRML
                     VPFAEYVPQADVIPWPEWLAPRVTELTAGDSGQLFTIAPQMSVAALICWENLFSHLAR
                     ESVNGGAQLLVQLTNDVWFGPTAAPRQHNLMSVMRAVENRVPVVIASNAGPSQLIDGY
                     GRVVAATSNVFQESAITGAVALGGGGTVYSKTGDWFVLFVLAGAFCTYVLLKRAHPGR
                     YANRPSFNWIRGVPGSLVVASMLGRGRRVLGRATPER"
     gene            complement(6927..7568)
                     /locus_tag="PLS35_05515"
     CDS             complement(6927..7568)
                     /locus_tag="PLS35_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNI18147.1"
                     /translation="MTSSLLRSTVIMLGLLAGGVAPAWAANAPVGWAVNAIGTLTIIR
                     ADGVEEQLQGRKTLPLYEGDVLKTDASSQAYIQLKQGVNVALNEETSFKILSRWQKDK
                     PTTWILRLSQGEVWAKTAGDGPKAFEVETPVATAAVKETEFNLKVQPDGQSILTVIEG
                     VVQFGTAFGTCPIRTNTISYGVRGKKCTKPAESDAKAAKAWTTTLIPPSAPVQ"
     gene            complement(7664..8659)
                     /locus_tag="PLS35_05520"
     CDS             complement(7664..8659)
                     /locus_tag="PLS35_05520"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNI18148.1"
                     /translation="MSSSWARIGAATLGLIGLAGCAEVETVKAPIDIPSRIYTANESS
                     NDVSVIDATSFAPVGNIDSKNQSTHDIAVSRDGRRVYATNLASGRLSVMDAGTMETIA
                     SIYTGQRCHVVALTNDNKQAWVANIGDNNISIVDTETFRIVGTIPTGKGPTGLTFSHD
                     GKFAYVSTQGDKTVDIIDTATHQIVKSLQVGANPHFLVVGPKGYIWGTNTGGTDIYVI
                     DPATQEIAGTLEVGAKPQQIAFGFKGMQGPNVFVTVGGTNKVAMVPADPKNLKVLEEF
                     AVGEGPNGIWANPEGTRIFVGHDKGNEVRIIDTGTGQNLGTVPVGRKPIRVVVSR"
     gene            complement(8727..11822)
                     /locus_tag="PLS35_05525"
     CDS             complement(8727..11822)
                     /locus_tag="PLS35_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI18149.1"
                     /translation="MRVNLFVVTALLCLFTLLVPSTWASPSTTAPRLLSGHASEILTL
                     AFSPNGTVLATGGTDQTIRLWDPESGQDLKLLRGHMGAVHALAFSPNGKFLASGSADT
                     SVRMWDLASGRELHAVTSNFGAVRAVAFSADGKMLAAGGNDGSLRFLDSANGKELRSV
                     RGQFGIVFSIAFSPDGTMVATGSSDMQIHLWDLATSRQRTVLTGHTGAVHAVAFAPVS
                     HLLASASADGTIRLWDVDSGQERLTLNGHKGEVFGISFAADGRTLASVGADGSVRVWD
                     VVSGRERYALTSHKGPARTVALSPDGNVVASGGRDKTVRLQPSTPSTVTANTAEKIAQ
                     RGNETGAAPSPPPMPEADLSIQPMELKAGQPVTLTVTVTNRGKGPLYRFQARSKSDDP
                     ALNGLLFYMGKVDPGQSVADAVTLPLPPDRPDGTIPLSLEFEEYNGFTPAPLQAALAL
                     TGQPRPRFAFTHQLLDDGSGQSVGNSDGRIQTGEAIDILVTIKNVGPVLAQHTTIDLT
                     VPQSRDFRLSQSTLDFGPLKPEETKIARVNLFVARDTKADELPLRLFIRERGMNVTLD
                     GTLKLAIDHRPAPQIIATNKLVSVHDASAKIHSGAGEETSVIASAAKDQGLAVTGELG
                     DWWRVQISPKETGWIAKRDAREPVERVKGDMPIPSIAGAPVVKLFQNAPPVIALASPS
                     DGADVTVDRILLAGAAASERGIARVEIRVNGHLLTQREARGIAVAPDAPAAQPNLEFS
                     ERLTLKEGKNEIVVSAIDRDNLSAQKVITVIRQVDRAKVWAVVIGISKYKTVHPLRYA
                     DVDAQAFHDYLMRNVGVPPENATLLLNEQATLMNLKRTLGTELKRKAAEKDTVIIYYA
                     GHGAPEADASASDDDGLEKYIVPYDADPKDLYSTGLPMREVETIFNRLAPERVIFISD
                     SCYSGAAAGRTFATASRRAVVSDTFLSRLSKGKGRIVLSASKASEVSEEREDLGHGVF
                     TYYLLEGLRGNADTDRDGVITVDEAYGYVSKKVPEVTGQNQHPVKKGEVEGQLILGQV
                     R"
     gene            complement(12123..12419)
                     /locus_tag="PLS35_05530"
     CDS             complement(12123..12419)
                     /locus_tag="PLS35_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18150.1"
                     /translation="MLSLILTLAFLILPYPLPVHVVLPATSAEAALLGGTIVSINQEA
                     LSLTILFPTGESRALSVRDARLLHGLSVGDHVTFELNDEHTLIKIAKLPTDPAN"
     gene            12554..13774
                     /locus_tag="PLS35_05535"
     CDS             12554..13774
                     /locus_tag="PLS35_05535"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNI18151.1"
                     /translation="MPPSKSDVPALLTVNAGSSSIKWALFHLGASPVRTASGRIERIG
                     IPDGIVTVTDRATGQDQRHIAQIPNHAAAVQILIDQLGQSGRGSSLQAIGHRVVHGGS
                     RYTNPEVISTAVMEELRRLSPYDPEHLPAELTIIDALRAQYPTVPQVACFDTGFHQHL
                     PAVARLLAIPRRYEKQGVRRYGFHGLSYAYLMEELERVAGHEAAQGKVVLAHLGNGAS
                     LAAVRDGVSIDTTMGFTPASGVPMSTRSGDLDPGLVSYLSQTEGMTAEEFHHMVHAQS
                     GLLGLSELSPDLRDLLAQERHDTRAAEAVAVFCYQGKKWIGAYAAALGGLDQLVFSGG
                     IGEHSSVVRARMCDGLAFLGIQLDQARNDAHEAVISAPGSRVTVRVIHTDEERHIAQS
                     VCRLLDLPLSGGTK"
     gene            13838..16213
                     /locus_tag="PLS35_05540"
     CDS             13838..16213
                     /locus_tag="PLS35_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNI18152.1"
                     /translation="MTQTTHTNQPLSAEMVQRIHAYWRAANYLSVGQIYLYDNPLLKQ
                     PLKREHIKPRLLGHWGTTPGLNFIYAHLNRIIKAQNLPVLYIAGPGHGGPGLVANVYL
                     EGTYSEVYPNISQDEAGLQRLFKQFSFPGGIPSHVAPETPGSIHEGGELGYSLAHAYG
                     AAFDHPELLVACVIGDGEAETGPLAGSWHANKFLNPVRDGAVLPILHLNGYKIAGPTV
                     LARMPPDELESLLIGYGHQPFFVEGDDPETMHQLMASTLDEIVATIRRIQQEARSHGF
                     TRRPRWPMIVLRTPKGWTGPKEVDGKLTEGTFRSHQVPMAEMDKPGHVELLERWLKSY
                     KPEELFDDTGKLMPELASLAPTGERRMGAIACANGGIILRDLHMPDFRQYAVPVVKPG
                     AEEAEATRVQGLFLRDVLKLNMRNFRIFGPDETASNRWGAVFEVTDRCSTEEILPTDE
                     HVSHDGRVMEILSEHLCQGWLEGYLLTGRHGFMNCYEAFIHIVDSMFNQHAKWLKTSR
                     EIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDHVVNKKAEVIRVYLPPDANTLL
                     SVTDHCLRSRDYVNVVVAGKQPAPQWLDMESAILHCTAGIGIWEWASNDRGSDPDVVM
                     ACCGDVPTMETLAAVEILRVAQPNLKVRVVNVVDLMKLQPASEHPHGMSDKEFDALFT
                     TDKPIIFAFHGYPWLIHRLTYRRTNHHNLHVRGYKEEGTTTTPFDMVVLNDLDRFHLV
                     ADVVDRVPQLGARAAYLKQEMRDKRMQHKQYIEIHGTDMPEIRNWKWSGKR"
     gene            16217..16903
                     /locus_tag="PLS35_05545"
     CDS             16217..16903
                     /locus_tag="PLS35_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNI18153.1"
                     /translation="MAVPRVVALFDVDNTLMDNDRITADLTQHLDEEIGARGERQYWA
                     IFEELREKLGYVDYLGALQRYRQEYPRDPHLLALSRFLLNYPFTERVFPQVHEVIRQT
                     AQWAKPVILSDGDVVFQPHKVDRSGLFDAVQGHVLIYIHKEQELDDVEQRYPADHYVL
                     IDDKLRILTAVKARWGARVTTVFPRQGHYAKDPEVLRTYPAADLSIDRIGDLLTLDRS
                     ALLAAAKPRT"
     gene            16958..18019
                     /locus_tag="PLS35_05550"
     CDS             16958..18019
                     /locus_tag="PLS35_05550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI18154.1"
                     /translation="MTPKTPVNRIWSLMLGRRQLLKGLGVAGSFMALGGPGRLAEALA
                     ADSPATGTSSGDIPRRALGKTGVQVSALCFGGAHWGRIQDDGEAIRLLHAAIDAGVNF
                     FDNAWEYNGGRSEELMGKALQGKRQQVVLMTKVCSHGRDKKVALQQLDESLRRLKTDY
                     LDLWQIHEVVYEDDPDRHFAPNGATEALLEAKRQGKVRFIGFTGHKHPDIHLKMLSHD
                     FPFDTCQMPLNVFDGTYRSFEREVLPVLNQRGIAPLGMKSLTGNAEPIKQGIVQPEEA
                     IRYVLSLPIASLVSGIDSPQVLKQNLDIARRFTPMTIAEMEGLRARVAMFAMDGRYEL
                     FKSTNRYDGPIGRQQHGIS"
     gene            complement(18106..19692)
                     /locus_tag="PLS35_05555"
     CDS             complement(18106..19692)
                     /locus_tag="PLS35_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18155.1"
                     /translation="MAHSFSDEQILQQSIRRRWGAIDWDLHFPWWRRPAKVRILMYAD
                     GSVHFNGGSFLGLQYVYNLLKTRAYSYVDFAIDFAHRTGGDPSATIAGARQLTDPALD
                     IMNKYDEIWFFGIESTPNLTAAEVALLTQFMAGPKFGGVLVTGDHDNLGRGISGQIPR
                     AGVMRRYPAPPDYGPTWNTTLEDGPDAGSTFDFDDQSDDTPQSIRWKRYTIASPLLLT
                     RRYRPHPVLCGPDGPINFLPDHQHEGEALAPTPVSGDPAWPTKGGHQETPEVIAWGRI
                     KDPNATKHGQEIGVISAYNGHNVDVGRILADSTWHHWFDINLTGRFAPPSPYSGFDET
                     PAGQLALKKIDAYFLNCGVWLAPPARQIEMRNAAWWSILWTDRMVELSPAASLTRFGE
                     EAIDALGGRASRCTVSEFIFKEPIFKEKIPRHEWPMLFDRFQLIDLPFERYVAGGILR
                     QLLHEVGPHNPKLTFPGKPAADEVLDKAIRTGTEEGLMALSKDLRREARMAVRLADSG
                     FLASALEDRVQRVTEETVTA"
     gene            complement(20094..21605)
                     /locus_tag="PLS35_05560"
     CDS             complement(20094..21605)
                     /locus_tag="PLS35_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HNI18156.1"
                     /translation="MLSVLRNNHLIRMITLVSALASMSPGMSLSQDPPIDTTTDRSDR
                     DLVGPVNHADRTESRANTSDRSSTLNWETREGRSYLIPAAEILAYLFLLNQFDRHFTE
                     PMSDYRTTGSTIRQHLTDSKWVLDNDQFSVNQFLHPYSGSIYFGLARSSGLNFWESSL
                     YSVAGSFLWEIGGEKTNPSINDMIATPIGGTFLGEPLFRMANLLLESDDGRPGFWREL
                     GAAIISPPTGFNRLVFGNRFDAVYPSNRPATFIRLAGGGTLTSSSHNVASNVNEHGAV
                     GEFTLTYGLPGKPGYSYARPFDYFDFHVSAATANTLETITSRGLLAGKTYASGDTTRG
                     IWGLFGSYDYISPQVFRVSSTALSLGTVWQSWLADELALQGTFLGGAGYGAAGSIQRT
                     EERDYHYGTTPQALLALRLIYANRAMLDITGREYYVSDFLSPERHGQENIVRAESSLT
                     LRVFDRHGVALRYSLSHRDAHYPSVEFRNQTVTAVSLMYVILGDSGFGAVEWR"
     gene            complement(21715..22614)
                     /locus_tag="PLS35_05565"
     CDS             complement(21715..22614)
                     /locus_tag="PLS35_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNI18157.1"
                     /translation="MAPRVTHRAGTYSPHRRRSRRRLTFTSLWRFTGRRWRDLRAFLR
                     AWGNTHPLPRLLIGIILIACLWGTLNWTYHAINKPTEVLFPLEDALDKNPAATWQEYG
                     PLFREHSTAIITPDLLAALAQVEGSGNPVARTYWRWRLSWNPFDWYRPASSAVGMFQL
                     TDGTFREAKRYCIHNHVVVEDGPWNDMHACWFNSFYTRIVPSHAIELTAALLDRQVAA
                     AIGSRRSASISRQQKQDLAAVIHLCGAGAGHAFVARGFHLAPHQKCGDHHASSYLARV
                     NGLKVQFARLAAGSSLQRLIKPR"
     gene            complement(22727..23509)
                     /locus_tag="PLS35_05570"
     CDS             complement(22727..23509)
                     /locus_tag="PLS35_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18158.1"
                     /translation="MIRVDITGEVEPQDVIQAIAALRENDQARQELLALRAETDRLQA
                     QVDDTNRALAAASTPYDVQQYTDQRQDMLDQLQANALLSQAWTSWTYPTLGFYSYPWI
                     GVSGINGLLLQAQRLSPHHRHLPLAQRTIMANLSAPPASPPVSTAPRASLLVPPVPHV
                     HHQQAAPLLNSQGQQAKVGDVVVIPTPRSIPSTPQYTPHAPPYQVHPNHFWRPTPPNV
                     QSLSSVPPQHSPSVSMAPRSSGGYAGHSGGGHSHSSGGHHGR"
     gene            complement(23513..24013)
                     /locus_tag="PLS35_05575"
     CDS             complement(23513..24013)
                     /locus_tag="PLS35_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18159.1"
                     /translation="MFFSRSTPTTAALLVLWLSVLPTAPSFASTTIYSYTNDEGVQTF
                     TNELASVPEKYRPQLTQRSFDTPARPETPAPDMASELRPRSAEARTITARGEYRMGDH
                     DNRADAVRLATEAAKQDALEQVATYVERITEVKDLNVTRDDIRSFTAGVVRVIDQTVT
                     TRLEQD"
     gene            24216..>24682
                     /locus_tag="PLS35_05580"
     CDS             24216..>24682
                     /locus_tag="PLS35_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18160.1"
                     /translation="MLVVKRIIAVVFGLVPLVLIAAGVAMVFRELRPTDPLLYSVQQD
                     GELPEAFMFAAVGCAGLFGCHRLWRADAGWKWTLMPIAVMVAAIAIPNLLYGHHGSPS
                     DRAYSNMFLRLGTFAVSSKQLAQEQGQFTCDPSVKLFGLSRFTQNGQPLPYVI"
BASE COUNT         4797 a         6972 c         7738 g         5175 t
ORIGIN      
        1 ctggctcgca cttgccagcg tccggagtag gcatctgtga cgatcggagc cacgaggtat
       61 ccctgggtga gtttcgtggt gtttacagcc atgatgctga cgaaaggata ggaatctttc
      121 tgtcccggcg gtgcatcgag gtagcgcagt tccaactccg ccacattggt cacgccgacc
      181 gcaggaggaa tggcggcaat gaaaagctgt ccctgcgtga cccgttcacc gggcaccggc
      241 ttggtgatca cgaacaaatc atcaggcact tgcagcttct tggcctgcgg attgaatggc
      301 acagatgggg gcggagtttg tacgagtgga gtggcgagct ttcccgccgg tgcaccgagc
      361 cgcgcaggga tcggggcgca gatcagtccg agagaatcga tgaacagccc ggcacggacg
      421 cgaaagcctg aggcgatctc gccaggcggg cagccatgtg gatacggtcc tttcgtcact
      481 cgttcttcgg aagccgcagt ggggttgcca aaatcgaaga ggccgatcga ctgagcaggg
      541 ccgagtgaag cacagtggat ttcaacatac tgcacgggcg gattgggtga tctgaccgtt
      601 tcgacatacc acgattggat agcgagggtc ttgttgccgg tgccccaaca ggttcgctga
      661 cggtcttggc cgcctccgct ggcgccgaac gacggcccga ccgcgggtaa tccaaacgcc
      721 tgtgatcccc tcagccaagg cgcgcagatc ggcgagatgc gatcgaccca ttcgcccgtt
      781 ctacctgcca ggcccacaag ataggagcct tgcgggcatt cggcccggaa gagagagccg
      841 ccggggccac cgattaactc cagatcggtc gccgaggaga gcgaggggaa gaagcccagg
      901 ctcaggacga gcagtgcacc agacaatacg tacaagccaa ggccgttcac gtctggccgc
      961 aaccgcttag ctgagcgagc aggacatcga gccatggatc agtttcctat ttggacgaag
     1021 gacccgtgcc cggcgtttct tgcgaaggct ggtcgccttg ctcgatgcct cgtcccagaa
     1081 tgccggggac tttcttgaat tggcggatga cttgattggt tttgttctgt gcctggtagg
     1141 cgccgaaccg tcggttgagg ctattcaccg cgtcttgatt catgaacgac gtgaatggac
     1201 gccgcctggc tcgaacgata tcgacaatat tgggagccaa cggttcgggc ctgtagcctt
     1261 gcgcattggg tgccgtacga tccaatggtg catcaactgt aggtacgatc tggtcgcact
     1321 gcacagttgg attcagccag gggtcggagc tgcagtgcat cgtcgttttg aagcggccgt
     1381 aggggcgtga gggttcgctg atcttcgggg ccggcacgag aatgtcctcc gccgcctggc
     1441 cggtcgtggg atcgaatgtc ccgactcctg tccacggaac cgacaccctg atcgctagtc
     1501 tggtttgggc caggttgcaa gaacctgaga agatatacgt gtaacgtgtc gcgacaaagg
     1561 tggcgttctt gggttcgctg ccgtaacagg taggatcgtt gggggcgacc aggtcgcctt
     1621 tcgcatgcgc ttccaacggt gacaataccg tgagcgaaaa cccgatgccg gcaatcaatg
     1681 accagttcat aatgtctccc tgcgtggcta aaggtacaca cttattctgc cggcgtagct
     1741 gccgaatcac tgggctcgct cttctctcga ttgaggccgc gaggccggat gactgacgtc
     1801 ccggctccga atcgctccga tggacttttg agtaacggcg aggccggcga tggggtctga
     1861 atcgattggt tctgttggtt cggcacgagc gtgaacgacg ctggagcgct ccatggtcct
     1921 gccactgccc cttggccgat catccgcaca cgcatttccc agcggccata gttgagccgc
     1981 gtgactttcg ggtcgatgta ttggccttgc agcagttttg ccgtgtccat ggcgggaatc
     2041 gtattgacga aacgcggctg tcctgcgggc gtatcgagcc acgcgaattc caactccgcc
     2101 acgggctcct tcccaatgag cggcggctga attttgacga gcaactgacc gacgaccacc
     2161 gtcgcgcccg acgccggact gaggatgcgg ggcatctgtc gcgagagaat ggcatcagag
     2221 gtgttcggcc cgcctggagt gaaaggagca accccgggtc cttgaggttt tgacagggta
     2281 gtctgcgacg gatcgaaggt ttgttgcgcg gccatggcga tgtgatattg cttgtcgatc
     2341 gctgcgcgct gtgcgggatt gagtttgaat gataagggca ggtttggagc ctcctgcagc
     2401 aactgctgat aggccatcga cacatatccc ggaaatgaag gtggcgaggt ctttctcgtt
     2461 accaggcggc aagggccgtt gggcatgatg cgccaggggt ctgagccgca ttgcatggtg
     2521 gagctcacgg ttcctgtggc cgtagttttt ccgaactcgc tcaacgccag gttcatcgtt
     2581 tcaaaggtcg ccttcgtatt gggctgatgg cttgcctgca cggtccagtg cacggcgaca
     2641 ttgttcacct gtgcggtgcc acctctcgat gacgtttcaa agaggaggca ttgacctgtg
     2701 atcgcataat cggaactcag tggcttgtgc gttccgttga ttgtgttcac ggagcaggtc
     2761 atggagagtt ggcccacaac agctggttca tcggccacgg cgacttcggt ggaaaccgct
     2821 aaggcgcaat gcaacacaac gatcccgagc actccgacga tccaggtcag gctcatgttt
     2881 ctagctggtg caggtgtcat cgaatccttc ctggtatcat gcgccggacc gctgccacta
     2941 ttattttgcg aacgatttgg tggccggttg gagaatcttg ttcgtcggcg gagccatgac
     3001 gccgaactcc acccagtcgc tccatccgga ttgttgagga gtggtcactt gggcgcttaa
     3061 ccgccatgcg ccggggatcg acaagaaggc cggcggggcc cccgcgccga agccggtata
     3121 ccctgtcgac gactccgctt gagcggcccc cacgggaagt gtggcatggg gaacccaaat
     3181 tccgttgggg tcttttcgct cgagccgcac catgtatccg gtcacgctct tcgcggtctt
     3241 gacgggggtt ccgtcgagac ggacttccgt atcggcccat ggtggagggg gcgctaactt
     3301 gatcgagatg ggagattggt taaagaaccg ctgtcccgcg gtgggcgcag tgatgatcgg
     3361 agagagactc atccggtaag gcgtcagggt ctgagttgcc gaacgcagcc gtttctcggc
     3421 ctgcgccttg gccaatgccg ccgcctcggc cttcaaatcc gcgtcccgtt tcgccgccaa
     3481 agccgtgcgt tctgccggca tcaagaccca agccgacatg ggcttgtagg ctctccatgc
     3541 attgaacaga tcgctgagag ttgggagagg tttcccatcc tggtactttc gggggtaggc
     3601 ctcggaggga ctattgtcca ttcggttcac gatctgacac ttcacgcgaa gactttccgg
     3661 gccgaacgga ggcccgtcca tcgtgagcca gggatcggat gggcatgaat aggttgtgcg
     3721 ccaggtccca tatgggtgac tcggttgcga gatcggagcc gccgcgattt caatcacctc
     3781 ctcggcgatg ttattggcat aggcaccctt caccgtgtag ggaaacgtcc tcggcgctcc
     3841 cagtgatccg ggtgtggtgc aagtgccggt cgagacgtat tgcgccttgg tggaagagaa
     3901 gctgccgacc atctgttgtg tcgcgctgct gcagggcgcc gagccgacgc cgttgaatcc
     3961 aaatccgaaa cacatggacg cgagggccac gatcaggacg agcccggcta cgacagtcat
     4021 ggcgtgccga tgccaaatat tcatgtggag cctcattgat gaaaggtatc ttcctagtgc
     4081 ttcctatggc ccgaacgtct tcggcgcccg ttgaatcgcc ttgttcgtgt tcatcacgac
     4141 aaattccgca ggctggctcc agccggtttg ttgcggggcg gagacctgag cggtaatccg
     4201 atacgttccg ggagaggcca tcaaagaaag gcttcgtcca tccggcccgc cggcgcccca
     4261 tccaaggtac ccggaaggag acgatgcctg ggcgaggccg atcgggaggg cggtgacaag
     4321 agcccaagtc ccgcctgggt tcttccgttc gatttttact gtgtagagag tgaccgtcac
     4381 ccgtcccgga ggcggcgcaa gtttgattgg cacggttgtc atgccgaggt agtgcgatcc
     4441 ggccgtcggg gagaccacaa aaggggcaag gttctcgagc agggtcgacc ccggctgcac
     4501 ctttttctga agctgctgag cctcttttcg agccgcttct tgtgcgagac gcgccgtttc
     4561 ggcaagggcc tgttgttccg cctgtagatc tcttggtcgt tgggccacca gagccgcccg
     4621 atcgtaagag aacccggtgg tattaggttt caacgtctgt tcgaagccgt tccgcaggta
     4681 ggtgacgatg taatcgagac tttgcacatc tccggtggaa gaaaagaccg gctgggtgca
     4741 ggccgctttg cccatcacca gccagggatc ttccacgcac ccaagcctgg tttcgatttt
     4801 cccccgaagc ggcgggttcc cgtttaattg gatcgtttct gccaccttgc cttgcccggg
     4861 ggcataggca ccttccgaag tccatggaat cgggatcact tccacatggt cctcgtactt
     4921 cagttgctga tcgcaccgtc cggtcatgcg ataggtggat ttcgccgccg tgaactttgc
     4981 ttgaatggag tccagatggc agctgaccgt cgtcatcgga tagatcctca cgacgacgcc
     5041 agcagcactg gcatcaccgg caatggcgga cagcacgaac aacagtacgc cccacatgtg
     5101 tctctgcatg aagatccttc cttatcctga ttcaaatgat ggcgcgtgat ctttcttatg
     5161 gtcatctctc cggagtcgcc cgtcccagta cgcgccggcc tcgtccaagc atggaagcaa
     5221 ccaccaagga tccggggacc cccctgatcc aattgaacga gggccggttc gcgtatctac
     5281 ccgggtgtgc tcgcttcaag agaacatacg tgcagaatgc accggcgagc acgaacagaa
     5341 cgaaccaatc cccggtcttc gagtagacgg tgcctcctcc gccgagggcc acagcgcccg
     5401 tgatggccga ttcctgaaag acgttcgatg tggcggcgac cacccggcca tacccatcga
     5461 tgagttgcga tggacctgcg ttcgaggcga tcacgaccgg cacacgattt tccacggctc
     5521 gcatgacgga catgagattg tgttgccggg gggcagcggt ggggccgaac cagacgtcgt
     5581 tcgtcagttg aaccagaagc tgtgccccgc cgttgacgga ttcccgggcg aggtgtgaga
     5641 agagattttc ccaacagatc aatgcggcga cggacatctg cggcgcgatc gtaaacagct
     5701 gaccactgtc accagcagtc agttcggtga cgcgcggagc aagccattcg ggccagggaa
     5761 tgacatcggc ctgcggcaca tattcggcaa agggcacgag catgcgtttg cgatagggag
     5821 gcgaggtcgc gccactcggc tggaatagat gagcggtgtt gaacagccgt cgtccgatgg
     5881 tgagcagatt gtcacccgtg gcgaattttt ccacctcggc tgccccgagg atcaagggca
     5941 cggcggtgtc gctgctcaac tgctggaggc gatgcagtag agccgggtcg gacacatcgc
     6001 cggggatggc gctttccggc cagacgatca aatccggtcg cgccgaagcc gcctctcgcg
     6061 tcaaccgctc aagacgggcc aggttggcct cccgccctgc ttctgtcgtg cggtcgccga
     6121 cttgaatgtt gggttgaaga gccgcaaccc tgatggatcg tcctgaggca ggcgagaaga
     6181 gttcggtggc gccccaggcg tgtgccgtga caaggatgag gagcgcgact gtcgcgtatg
     6241 ttcgctctcg tctcaagcac agggcggcaa gggcggcatt gcccagtgcc accaggaacg
     6301 tgacgccatg ttctccaatc atgctggctg tctgcagcag cgcgagatta cgatgctgcg
     6361 tctgagcgag cgtgccccag gggagcgcta aaaacccggc atgtccacgg agataatcga
     6421 tgagtagcca gaggaccggc gcgcacagga gaagcggaat gtctcggcga agtgcccagg
     6481 aagtggccga tgcccatatt gccggatagg ctccgacgta cagagctagc agcacggcat
     6541 gacgtagatc gaagctaggc acttcgaaca gccagccata catgccgaag ctagccagga
     6601 cgccggtcag cagtcctagc gcggccgccc gaccagggct gagtccctgg atggccagga
     6661 gaatcgggac gagtgcgccc caacccaacc agccgatatc gggggagggc agggatacga
     6721 cgagtagtgc cgcagatagc agcgccagcg cgataggtgt gatgccgtca gttacgcgca
     6781 gccgttgatg tgccgtcatc ccgactcccc ctgtcggcct caggcgctcc attcagatag
     6841 cgactattcc ccataccgtc ggaccggctc tgatcgactc aaccgcccga ggtaagtctg
     6901 ccgatcgtcg gcacaggttc attctattat tgcaccggcg ccgacggcgg aatgagcgtg
     6961 gtggtccagg ccttcgccgc cttcgcatcg ctctccgcgg gcttcgtgca tttcttgccg
     7021 cgcacgccgt aactgatcgt gttggtccgg atcggacagg ttccgaaggc ggtgccgaat
     7081 tggacgacgc cttcgatcac cgtcagaatg ctttgcccgt ccggctgcac tttcaaattg
     7141 aattccgtct ctttgacggc tgcggtggcc acgggggttt cgacctcgaa ggccttcggg
     7201 ccatcaccgg cggtcttggc ccacacttcg ccttgactaa gccggaggat ccatgtggtc
     7261 ggtttgtcct tttgccagcg ggagagaatc ttgaacgagg tctcctcgtt cagcgccacg
     7321 ttgacgcctt gtttcagttg aatgtaggcc tggctggaag cgtccgtctt gaggacgtca
     7381 ccttcataga gcggcagggt cttccggcct tggagctgtt cctctacgcc gtcggcgcgg
     7441 atgatggtca aggtgccgat agcattgacc gcccatccta ccggagcatt ggcggcccat
     7501 gcgggtgcca ctccaccagc aagtaatccc aacatgatga ccgtagatct gagtaatgat
     7561 gacgtcataa attcctcttc gcgtcttgtc gccgcgcgtt atgtcagttc agtgtgtcga
     7621 acgcccgcat ccccaagatg cgcgcggtac atgcgtccgg ccgctatctc gagaccacga
     7681 ctcgaatcgg cttgcggccg accggcaccg tgccgagatt ttgtcccgtg cccgtgtcga
     7741 tgatgcgcac ttcgttaccc ttgtcgtgac cgacgaagat gcgggtgccc tccgggtttg
     7801 cccagatgcc gttcggccct tcacccacgg cgaattcctc caggaccttc aggttcttgg
     7861 gatctgccgg caccatcgcc accttgttgg tcccgccgac ggtgacaaac acattaggcc
     7921 cctgcatgcc tttgaacccg aacgcgatct gctgcggctt cgcaccgacc tctaatgtgc
     7981 cggcgatttc ctgcgtggcc ggatcgatca cgtagatgtc cgttccgccg gtattggtcc
     8041 cccagatgta ccccttgggg ccgaccacga ggaagtgagg attggcgcct acctgcagag
     8101 atttcacgat ttggtgcgtg gcggtatcga tgatgtcgac ggtcttgtcg ccctgggtgc
     8161 tgacgtaggc gaatttgccg tcgtgcgaaa aagtcaggcc ggtcggtccc ttgccggtgg
     8221 ggatggtgcc cacaatcctg aaggtctccg tatccacgat cgaaatattg ttgtcgccga
     8281 tgttggcgac ccacgcctgt ttattgtcgt tggtgagtgc caccacatga cagcgttgcc
     8341 cggtataaat ggatgcgatg gtttccatcg tgcctgcgtc cattactgag agccggccgc
     8401 tggcgaggtt cgtggcatag acgcgccggc catcgcgcga gacggcgatg tcatgcgtgg
     8461 attggttttt tgaatcgatg ttgccgaccg gcgcaaagct cgtcgcgtca atgaccgaga
     8521 cgtcgttcga gctttcattg gcggtataaa tgcgcgatgg aatatcgatc ggcgccttga
     8581 cggtttcgac ttcggcgcaa ccggccagac cgatgagccc aagcgtggcc gcgccgatgc
     8641 gtgcccatga cgatgacata acaatcctcc ttactgatct atgaacatga cagaatgata
     8701 aagtgcccta tataagcgac gatgtgctac cgcacctgtc ccaggatcaa ttgcccttcc
     8761 acttcaccct tcttcacggg atgctgattc tgccccgtga cctccggcac cttcttggag
     8821 acgtagccgt aggcttcatc gacggtaatg acgccgtcgc gatcggtgtc cgcgttgccg
     8881 cgcaggcctt cgagcaaata gtacgtgaac acgccgtgtc ccaggtcttc gcgctcctcg
     8941 ctgacttcgc tggccttgct cgcactgagg acgatacgcc ctttcccctt tgaaagccgg
     9001 ctcaggaatg tgtcggagac cacggcgcgg cgcgaggcgg tggcgaaggt gcggcccgcc
     9061 gcggcgccgc tgtagcagga atcgctgata aaaatcacgc gctcgggcgc aagccggttg
     9121 aaaatcgtct cgacttcccg catcggcagg ccggtcgagt agaggtcctt cgggtccgca
     9181 tcgtagggca cgatgtattt ttcgaggccg tcgtcgtccg aggcagacgc atccgcttcc
     9241 ggcgcgccat gaccggcgta ataaatgatg accgtgtcct tctccgccgc tttacgcttc
     9301 agttccgttc cgagtgtgcg tttcaaattc atcaacgtcg cctgttcgtt caacagtagc
     9361 gtggcgttct ccggcggaac gccgacattg cgcatcaaat agtcatggaa ggcctgcgca
     9421 tccacatccg cataccggag cggatggacc gtcttatatt tcgagatgcc gatcaccacg
     9481 gcccagacct tggcacggtc cacctgccgg ataacggtga tgaccttctg tgccgaaaga
     9541 ttgtcgcggt cgatggcgga cacgacgatt tcgtttttgc cctccttcag cgtcaggcgt
     9601 tccgaaaatt cgaggttcgg ttgtgccgcc ggcgcatccg gcgcgacggc aatccctctc
     9661 gcctcacgct gggtcaggag gtggccgttg acgcgaatct ccacccgcgc gatgcctcgt
     9721 tcactggccg cggccccggc aagcagaatc cggtccaccg ttacgtctgc tccatccgag
     9781 ggggaggcga gtgcgatgac cggaggggcg ttctggaaca atttcacgac cggcgcaccg
     9841 gcaatcgaag gaatcggcat gtcgcctttc acacgctcga caggttcacg cgcgtcgcgt
     9901 ttggcgatcc atccggtttc tttgggggag atctgcacgc gccaccagtc gccgagttcg
     9961 ccggtcacgg ccaggccttg atccttggcg gcggacgcga tgaccgaagt ttcttcgccg
    10021 gcgccgctgt gaattttggc cgaggcgtca tgcaccgaga cgagtttgtt ggtcgcgatg
    10081 atttgcggcg cgggccggtg atcgatggcg agcttcagtg tgccatccag cgtgacgttc
    10141 atgccgcgct cgcgaatgaa gagtcgcagg gggagttcat cggccttcgt gtcgcgcgcg
    10201 acaaacagat tcacgcgggc aattttggtt tcctctggct tcaaaggccc gaagtcgagt
    10261 gtgctctggc tcaggcggaa gtcacgtgac tggggcaccg tgagatcgat cgttgtgtgc
    10321 tgagccagga cggggccgac gttcttgatc gtgaccagta tgtcgatggc ctcgccggtc
    10381 tggatgcggc catcgctgtt cccgaccgat tggcctgatc cgtcatccag caattggtgg
    10441 gtgaaggcaa accgtggtcg gggttgtccc gtgagggcca gcgctgcctg cagcggggca
    10501 ggcgtgaagc cgttgtattc ttcgaattca agcgagaggg gaatggtgcc gtccggccgg
    10561 tcgggcggaa ggggcagcgt gacggcatcg gccacggact gtcctggatc gaccttcccc
    10621 atataaaaca gcaggccgtt caatgccgga tcgtcgcttt tgcttctggc ctggaagcgg
    10681 tacagagggc ctttgcccct gttggtgacg gtcacggtca gggtgaccgg ttggcccgct
    10741 tttaattcca tcggctgaat ggagaggtct gcttccggca tgggtggtgg agagggggcg
    10801 gcgccggttt cgttgccgcg ttgtgcgatc ttctccgccg tatttgccgt gacggttgaa
    10861 ggagtgctgg gctgcagtcg cacggtttta tctcgcccgc cactcgccac cacgttgccg
    10921 tcgggagaca gggccaccgt cctcgccggt cctttgtggc ttgtgagggc gtagcgctcg
    10981 cggcctgaga ccacatccca cacccggacg ctaccatcgg ctccgacgga ggcgagcgtg
    11041 cggccatcgg ccgcgaagga gatgccgaag acctcgccct tatggccgtt cagggtcaag
    11101 cgttcctggc cgctgtcgac atcccagaga cggatcgtgc cgtcggcgga agcgctggcg
    11161 aggaggtggc tgaccggagc gaaggccacg gcgtgcacgg cgccagtatg gccggtgagc
    11221 accgttcgct gccggctggt ggccagatcc cagaggtgga tctgcatatc gctactgccc
    11281 gtcgccacca tggtgccgtc cggggagaag gcaatgctga agacaatccc gaactgcccc
    11341 cgcaccgatc gaagttcttt tccgtttgca ctgtctaaaa accggaggct gccgtcattg
    11401 ccgcctgcgg ccagcatctt gccgtcggcg gaaaaggcaa cggcgcggac tgcgccgaaa
    11461 ttcgaggtca cggcgtgcag ttcacggcct gatgcgaggt cccacatgcg cactgaggta
    11521 tctgcgcttc ccgaggccag aaattttccg ttcggagaga aggccagggc atggacggcg
    11581 cccatgtgac cgcgcagcag cttcagatcc tgtccactct cgggatccca gagacgaatg
    11641 gtttgatcgg tcccgccggt ggcaaggacg gttccattgg gtgaaaacgc cagggtcagg
    11701 atctcactgg cgtgaccgct gagcaggcgt ggcgctgtgg tggaggggct ggcccatgtg
    11761 gagggtacca ggagcgtgaa gaggcacagg agcgccgtga caacgaacag gttcacgcgc
    11821 atgggggcta tccttttccg acggaggtgc cggcctgtct caccgttcgg atcagatgtc
    11881 tcacaaaaac cagtcttccg cttgtcctgt tcctgtctgt cgggtttccc tgcctggagg
    11941 ttcacccgtt ttgcggcggc accagtgtat ttttcggcat tcccgattgt caatccacag
    12001 gcgcgggcag ccggtcaaat acaggtattg gcaggtatac gcttgctgta cggtcttgat
    12061 tcagtgctca agagtggaga ctcgtagagg gcacgtaagt tcaatgtgga ggagggcgac
    12121 aatcagttgg ccggatcagt cggcagcttg gcgatcttga tcaaggtgtg ttcatcattg
    12181 agttcgaagg tgacatggtc cccgacgctg aggccatgca gaaggcgggc atcccgaacg
    12241 gaaagtgcgc gagactctcc ggtggggaaa agaatggtca ggctgagcgc ttcctgattg
    12301 atcgagacaa tcgtgccgcc cagcagagcg gcctctgcag atgtggctgg gaggacgaca
    12361 tgcaccggca ggggataggg gaggattaga aaagcgaggg tgaggatgag cgaaagcatc
    12421 cgacacgata gcgcagctcg tgcctgtgtg gaactagcgt ttcccctata gcgacagtgc
    12481 gcagaagacc tacagcaaga gccggggatt ttccggttgc gctgatcgta tggtcgatct
    12541 atggtgggag gacatgccgc catccaaatc agatgtccca gccctgctga ccgtcaacgc
    12601 cgggtcgtcc agcatcaaat gggcgttgtt ccacctggga gcgtcgcctg tccggacggc
    12661 ctctgggcga atcgagcgca tcgggatacc tgacggcatc gtgacggtga ctgatagagc
    12721 aaccggccag gatcagcgcc atatcgcaca gattccgaac cacgcggctg ccgttcagat
    12781 cctcatcgat caactcgggc agagcggccg gggatcgtcg ctacaggcca tcggccatcg
    12841 agtcgtgcat ggaggaagcc gttatacgaa tccggaagtg atctcgacgg cggtcatgga
    12901 agaattgcgt cggctcagcc cctacgatcc tgaacatctt ccggcggaac tcaccatcat
    12961 cgacgcgctg cgcgctcaat atccgaccgt ccctcaagtg gcttgtttcg atacgggctt
    13021 tcatcagcat ttgccggcgg tcgcgcggct gctggcgatt cctcgacggt acgaaaaaca
    13081 gggcgtccgt cgatacggct ttcacgggtt gtcctatgcc tacctcatgg aggaattgga
    13141 gcgggtggcc ggccatgagg ccgcgcaggg caaagtggtc ctggctcatt tgggaaacgg
    13201 agccagcctc gccgccgtgc gtgatggcgt gagcatcgac acgacgatgg gattcactcc
    13261 ggcctcaggg gtgccgatga gcaccaggtc cggcgatctg gacccgggtc tggtgtccta
    13321 cctctctcag accgagggga tgacggcgga ggagttccat cacatggtac acgcgcagtc
    13381 ggggctgttg ggattgtctg agctcagtcc tgacctacgg gatttgctgg cgcaggaacg
    13441 ccacgatacg cgcgccgctg aggccgtcgc ggtcttttgt tatcagggga aaaagtggat
    13501 cggtgcttat gcggcggcgc tcggtggatt ggaccagctc gtcttcagcg gcgggatcgg
    13561 cgaacattca tccgtcgtgc gggcgcgcat gtgtgacggc ctggcatttc tcggcattca
    13621 gttggaccag gccaggaacg acgcccatga agccgtcatc tctgcgcctg gtagccgggt
    13681 caccgtgcgg gtgatccata cggatgaaga acgccacatt gcacaatcgg tctgccggct
    13741 gttggacttg cccctcagtg gcgggacgaa atgaaccggg cgtcagacgg ataggcacga
    13801 ggcgaccggg tatgctcaca tctgaaagag gaacttcatg acacagacga cgcacacgaa
    13861 tcagccgctg agcgccgaaa tggttcaacg gatccatgcc tactggcgcg cggcgaacta
    13921 tctctccgtc gggcagattt acttatacga caacccgctg ctcaagcagc cgttgaagcg
    13981 ggaacatatc aagccgcgcc tgctcggcca ttgggggacc acgccggggc tgaatttcat
    14041 ttatgcccat cttaatcgca tcatcaaggc gcagaatctt ccggtgctct acattgccgg
    14101 gccaggtcat ggtggtccgg ggctggtcgc caatgtctat cttgagggca cctacagcga
    14161 ggtctatccc aacatctcgc aagacgaggc ggggctccag cggctgttca aacagttttc
    14221 attccccggc ggcattccga gccatgtcgc accggaaacg cccggctcca ttcacgaggg
    14281 tggcgagttg ggatattccc tggcccatgc ctatggcgcg gccttcgatc atcctgaact
    14341 gttggtggca tgcgtcatcg gcgacgggga agcagaaacc gggccgctgg cgggcagttg
    14401 gcatgccaat aagtttctga accccgtgcg ggacggagcc gtcctgccga ttctgcatct
    14461 gaacggctac aaaatcgccg gccccaccgt gctcgcccgc atgccgccgg atgaactcga
    14521 atccctgctg atcggctatg gccatcagcc gttttttgtc gagggtgatg atccggagac
    14581 aatgcatcaa ttgatggcct caacgctcga tgaaatcgtg gcgactatcc ggcgcattca
    14641 gcaggaggca cgatcccatg gttttacccg tcggccgcgc tggcccatga ttgtgttacg
    14701 caccccgaag ggatggaccg gccccaaaga ggttgatggc aagttgacgg aagggacctt
    14761 ccgttcgcac caggtgccga tggccgagat ggataagccg gggcatgtcg agttgttgga
    14821 gcgctggctg aagagttaca aacccgaaga actgttcgac gacacaggaa agctgatgcc
    14881 ggagctggct tcgctggcgc cgaccggtga gcgacgcatg ggcgccatcg cctgtgcaaa
    14941 tggcggcatc atcctgcgcg atctgcacat gcccgacttt cggcaatacg ccgtgcccgt
    15001 ggtcaagcct ggtgcggagg aggccgaagc caccagggtg cagggactgt ttcttcgcga
    15061 cgtgttgaaa ctgaacatgc gcaacttccg gatcttcggg ccggacgaga cggcctcgaa
    15121 ccgctggggc gccgtgttcg aagtgaccga ccggtgctcg acggaagaga ttctccccac
    15181 tgacgaacat gtgtcccacg atggacgtgt gatggagatt ttgagcgagc acctctgcca
    15241 gggctggctc gagggctatc tgttgaccgg ccgccacggc tttatgaatt gctacgaagc
    15301 gttcattcac atcgttgatt ccatgttcaa ccagcatgcc aaatggttga agacctcgcg
    15361 cgagattccc tggcggcggc cgatcgcctc gctcaactat ctgttgacgt cccatgtctg
    15421 gcggcaggac cacaatgggt tcagccacca ggatccggga tttatcgacc atgtcgtgaa
    15481 taagaaggcg gaggtgattc gtgtctacct gccgccggat gccaacactc tgctgtcggt
    15541 caccgatcac tgcctgcgca gccgtgacta tgtgaacgtt gtcgtggcgg gaaaacagcc
    15601 ggcgccgcaa tggttggaca tggagtccgc catcctccat tgcacggccg gcatcggcat
    15661 ttgggaatgg gccagcaatg accgggggag cgacccggat gtcgtgatgg cctgttgcgg
    15721 cgatgtgccg accatggaga cgctggcggc cgtggagatt ctgcgtgtcg cccagcccaa
    15781 cctgaaggtg cgtgtggtca acgtggtcga tctgatgaag ctgcagcccg ccagcgaaca
    15841 tccgcacgga atgtccgaca aagagttcga tgcgctcttc accaccgata agccgatcat
    15901 cttcgctttc catgggtatc cctggttgat ccaccggctt acctaccggc ggacgaacca
    15961 tcacaacctg catgtgcgag gctacaaaga agaaggcacg acgacgacgc cgttcgatat
    16021 ggtggtgttg aatgatctcg accggttcca tctcgtagcc gacgttgtcg accgtgtgcc
    16081 gcaactcggg gcgcgggctg cctatctcaa gcaggagatg cgggataagc gcatgcagca
    16141 caagcagtac atcgagatcc atggcaccga catgcctgag atcaggaact ggaagtggag
    16201 cggaaagcgc taagttatgg cagtacctag ggtggtggcg ttgttcgatg tcgataatac
    16261 gttaatggac aacgaccgga ttacggccga ccttacgcaa cacctggatg aagaaatcgg
    16321 cgcgaggggg gagcgccagt attgggccat cttcgaagaa ttgcgggaaa agctcggata
    16381 cgtggattac ctgggcgcgc tgcagcgcta tcggcaggag tatccccgcg atccccatct
    16441 gttggccctg tcccggttcc tgctgaatta ccccttcacg gagcgcgtgt ttcctcaggt
    16501 gcatgaggtg attcgccaga cggctcagtg ggcgaaaccc gtgattcttt ccgatggcga
    16561 cgtggtgttt cagccgcaca aggtcgaccg ctccggtctc tttgacgcgg tccagggcca
    16621 tgtcctcatc tacatccaca aggagcagga gctggacgac gtcgagcagc ggtatccggc
    16681 cgaccactat gtcttgattg acgacaagtt gcgcattctt acggcggtga aagcccgttg
    16741 gggcgcgcgc gtgacgaccg tgttcccgcg gcagggacat tatgcgaagg acccggaggt
    16801 gctgcgaaca tacccggcgg ccgacctcag catcgatcgc atcggcgact tgctcacgct
    16861 cgatcggagc gcgcttctgg ccgccgcgaa gccacgtaca tgacgagcag tcggaaggtt
    16921 gtgtgcctat catccacctc tagcaggcag gtgattcatg acacccaaga cacccgtgaa
    16981 cagaatctgg tccctgatgc tcggccgccg tcaacttttg aaaggactgg gcgtggccgg
    17041 ctccttcatg gcgttaggag gtcccggccg gttggccgaa gcactcgccg cagactctcc
    17101 tgcgaccggc acctcctccg gagacattcc acgtcgggcg ttgggcaaaa ccggcgtgca
    17161 ggtctcggcc ctctgtttcg gcggcgccca ttggggccgc attcaggatg atggcgaggc
    17221 gattcgcttg ttacacgcgg ccatcgatgc gggcgtaaat tttttcgaca atgcctggga
    17281 gtacaacggc ggacggtccg aagagctgat gggcaaggcg ctgcagggaa aacggcagca
    17341 ggtagtgctg atgacgaaag tctgctcgca cgggcgtgac aagaaggtgg cgcttcagca
    17401 gctggatgag tcgctccgcc ggttgaagac cgattatctc gatctctggc agatccatga
    17461 agtggtctat gaagatgatc ccgatcggca tttcgcgccc aacggcgcga cggaagcctt
    17521 gctggaggcg aagcgacagg ggaaggtgcg gtttatcgga ttcacggggc acaaacatcc
    17581 tgatatccac ctgaagatgc tgtcgcatga ctttccattc gatacctgcc agatgccgtt
    17641 gaacgtcttc gacgggacct atcgcagctt tgagcgggaa gtgctcccgg tgctgaacca
    17701 gcggggcatt gcgccgttgg gcatgaagag tttgaccggt aatgccgagc ccatcaaaca
    17761 aggcatcgtt cagcctgagg aggccatccg gtacgtcttg agcttgccga ttgcgtcgtt
    17821 agtgagcggg atcgactcgc cccaggttct gaaacagaac ctcgatatcg cgcggcggtt
    17881 tacgcccatg accatcgccg aaatggaggg actgcgcgcc agagtcgcga tgtttgcgat
    17941 ggacgggcgg tacgaactgt tcaagtccac gaatcgctac gacggtccca ttggccgcca
    18001 gcagcacgga atctcttgac cggttcagcg tggtgcctag gcacaggtcc ggcactcgcc
    18061 tgtgaagacg cgagcgccgg cctatgttgg gttagatgta ttcgatcacg cagtgaccgt
    18121 ttcctcggtc actcgttgga ctcgatcctc taacgcactt gccagaaacc cactgtccgc
    18181 caatcgaaca gccattctcg cctccctgcg cagatccttg ctcaaagcca tcaagccctc
    18241 ttctgttcct gtgcggatgg ctttatccag gacttcatct gcagccggct tccccgggaa
    18301 cgtcaatttg ggattgtgtg ggccgacctc atgcaggagt tgtctgagaa tgccccctgc
    18361 gacgtatcgc tcgaatggta ggtcgatgag ttgaaagcga tcgaagagca tgggccattc
    18421 gtggcgcgga attttctctt tgaagatcgg ttctttaaag ataaattcgc tgactgtgca
    18481 acgtgatgct cggccgccga gcgcatcgat ggcttcttct ccgaaacgcg tcaggcttgc
    18541 cgcaggagac agttcaacca tacggtctgt ccagagaatt gaccaccaag cggcatttct
    18601 catttcaatt tgtcgtgccg gaggagcgag ccagactccg caattcagaa agtacgcgtc
    18661 gatctttttt aacgccagct ggccggctgg tgtttcatcg aacccggagt atggcgacgg
    18721 aggagcaaat cggcccgtca ggttgatgtc gaaccaatgg tgccaggtgg aatcggcaag
    18781 aatccggccg acatctacat tgtgaccgtt ataggccgat atcactccga tttcttgccc
    18841 atgtttggtg gcgttcgggt ctttaattct tccccatgca atgacttcag gagtctcttg
    18901 atggccgcct ttggttggcc atgcggggtc gcctgatact ggtgtggggg cgagagcttc
    18961 accttcgtgc tgatggtctg gcaggaaatt gatcggcccg tcgggtccgc ataacacggg
    19021 gtggggacga tagcgcctag tcaaaagcaa gggggaggcg atggtgtagc gcttccaacg
    19081 tattgattgt ggtgtatcat cggattggtc gtcgaaatcg aatgtagatc ctgcatcagg
    19141 tccatcttca agtgtggtgt tccacgtcgg tccgtaatcc ggcggcgcag gataccgtct
    19201 catgacgccc gcgcgcggga tttgtccgga gattcccctt cccaggttgt catgatcccc
    19261 cgtgaccaga acccctccga acttcggccc cgccatgaac tgtgtcagga gtgcgacctc
    19321 agccgcagtg agatttgggg tcgactcaat accgaagaac cagatctcat catacttgtt
    19381 cataatgtcg agtgcgggat cggtcaactg tctggcgccg gcaattgtcg ccgaagggtc
    19441 gcctccggtt cggtgagcaa agtcgatggc gaagtcgaca taagagtacg cacgagtctt
    19501 taatagattg tagacgtact gaaggccaag gaatgatccg ccgttgaagt gtacgctgcc
    19561 gtccgcgtac atgagaatcc tgaccttagc gggacgacgc caccagggga aatgaagatc
    19621 ccaatcgatc gctccccagc gtcgacgaat cgactgttgc agaatttgtt catccgagaa
    19681 tgaatgtgcc ataagacctc cttgtgtagt caaaagctcg tcatgagccc tggttaaaga
    19741 tgagactcgt tcgagtcacc acgctgattg agcgctgcat cattgaacga gaatgcgatt
    19801 cactagctag caagacctgc tccacaattg atgggaagaa aaacccatta cacaatcaca
    19861 gagttgaaat acggggtttg agaaatgtga atttctgcca gtgaatactg gtttattgat
    19921 tcagatacat tgagtatcgt ggcggatacg cattggcaat gatggcatgc tgtggcgacg
    19981 gatcaacgga agatcggcaa attcgaacgg gctatggcgt gggagaaagg ggggaatttg
    20041 atgaaacgac tagaccacca ttttaggaac atccctgaac agcggttagc cgctcatcgc
    20101 cattcgacgg cgccgaagcc ggaatccccg aggatgacat acatcagact gacggccgtg
    20161 actgtctgat tgcgaaattc tacgctcgga taatgtgcat cccggtggga gagagaatac
    20221 cgaagcgcca cgccgtgccg gtcgaacacc cgaagcgtca atgaagattc tgcccggacg
    20281 atattctcct gcccatgccg ttctggagag agaaagtcgc tgacgtaata ttcccggccc
    20341 gtaatgtcta gcatggcgcg gttcgcataa atcaggcgaa gcgccagcag cgcctgcggt
    20401 gtcgtcccat aatggtaatc ccgttcttcc gtgcgctgaa tgctcccggc cgctccatat
    20461 cctgcgccac ccaagaatgt tccttgaagt gccagttcgt cggctaacca ggactgccac
    20521 acggttccca gtgagagagc ggtgctggag acccggaaga cttgcggcga gatatagtca
    20581 tagctcccga acaaacccca gattccgcgg gttgtgtcgc cggatgcgta ggtcttcccc
    20641 gcgagcagcc cccgtgaggt gatggtttcc agggtattgg ccgtcgcggc ggagacatga
    20701 aaatcaaaat agtcgaacgg gcgtgcgtag ctgtagcctg gcttgccggg tagaccgtac
    20761 gtcagagtga attctcccac cgcgccgtgc tcgttcacgt tcgacgccac attgtggctg
    20821 ctcgaggtca gcgtgcctcc accagcgaga cgaatgaacg tcgcgggccg gttactgggg
    20881 tagaccgcat cgaagcggtt accgaatacc agccgattga atccggtggg cggtgaaatg
    20941 atggcggcgc ccagttcgcg ccaaaagccc gggcggccat cgtctgattc gagcaggaga
    21001 ttggccattc tgaataatgg ctcgcccagg aatgttccgc cgatgggggt cgcgatcatg
    21061 tcattgatcg aaggattcgt tttttcgccg ccgatttccc agaggaaact gccggcgacg
    21121 ctatacagcg acgactccca aaagttgagt ccggatgagc gggccagccc aaagtagatg
    21181 ctgccgctgt aggggtgcag gaattggttg acggaaaact ggtcgttgtc gagcacccat
    21241 ttggaatcgg tcaggtgttg acgaatggta ctgccggtcg tgcggtaatc tgacatcggt
    21301 tcggtaaagt gtcgatcgaa ctggttcaac aggaagagat acgcgagaat ttccgcagcg
    21361 gggatcagat aactgcggcc ctctctcgtc tcccaattga gtgtcgagga ccggtctgat
    21421 gtattagccc gactttctgt ccgatcagcg tgattgactg gtccgaccag gtcgcgatcg
    21481 ctacggtcag ttgtggtgtc gatcggcgga tcctgggaga gactcatgcc cggggacatt
    21541 gaagccagcg ccgaaaccag cgtgatcatt cggataaggt gattgttgcg caggacggac
    21601 agcatgctgt gtcagggctc ccaccgtgag ttgatcccat cagtccgctt cgggacactc
    21661 ggggaaaccc tagctcctga cgcagaatgc ctagttgcgc agagagatgc cgtgtcagcg
    21721 gggttttatc agccgctgaa ggctgctgcc ggcagcaagg cgggcgaact gtaccttcaa
    21781 gccgttgacc cgggcgaggt aactgctggc gtgatgatca ccgcatttct ggtggggcgc
    21841 gaggtggaac ccgcgcgcaa cgaacgcgtg cccggctccc gctccgcaga ggtggatgac
    21901 agccgcaagg tcctgcttct gttgcctgct gatagaggcg cttcgccggg aaccgatcgc
    21961 cgcggcgacc tgacgatcga gcaaggcggc ggtcagttcg atggcatggc tcggaacaat
    22021 gcgtgtgtag aagctgttga accaacaagc atgcatatca ttccaggggc cgtcttcaac
    22081 caccacatgg ttgtggatgc agtagcgttt cgcttctcgg aatgtgccat cggtcaactg
    22141 gaacatcccg acggcgctag aagccggtcg gtaccaatcg aacggattcc aggacaatcg
    22201 ccagcgccaa taggttcgtg ccacgggatt gcccgatcct tccacttgtg ccagcgcggc
    22261 gagaaggtcc ggggtgatga tggccgtcga atgctcacga aagagcggcc cgtattcctg
    22321 ccaggtggcc gcgggattct tatccagcgc atcctctaga ggaaacaaca cttcggtcgg
    22381 cttattgatc gcgtgatagg tccagttcag ggttccccag agacaggcga tgaggatgat
    22441 gccgataagc agacgcggga ggggatgcgt attgccccac gcgcgaagga acgctcgaag
    22501 gtcacgccag cggcgtccgg tgaaccgcca gagcgaggtg aatgtgaggc ggcgacggga
    22561 gcggcgtctg tggggggaat atgtgccggc acgatgagtc actcgaggcg ccatcgcctc
    22621 actatacccg aaatgggctc atgagctact cagttattgg ctacccgtat gtgggatcgc
    22681 tccccggaag cgaggcctcg cagagagctc ttgctgatgc gcgccgttat cgcccgtggt
    22741 ggccgccgct gctgtgggaa tggccgcctc cgctatgccc ggcataaccg ccggaagatc
    22801 ggggcgccat gctgaccgat ggactgtgtt gcggcggtac tgaggaaagg ctctgaacat
    22861 tcggcggggt ggggcgccag aaatgattcg ggtggacctg gtaaggcggc gcgtgcggcg
    22921 tgtattgcgg agtcgaggga atcgatcgtg gtgtcgggat gaccaccacg tcacctacct
    22981 tggcctgctg gccctggctg ttgaggagtg gcgcggcctg ctggtgatgc acgtgaggca
    23041 cagggggaac taacagcgag gcgcgcgggg ccgtggagac cggcggtgat gcgggtggag
    23101 cgctgaggtt cgccatgatg gttctctgcg ccagcgggag gtggcgatga tgcggcgaaa
    23161 gtcgctgcgc ttgcagcaac aggccgttga tgcctgatac gccgatccat gggtatgagt
    23221 agaagcccaa tgttggatat gtccaactcg tccaggcctg cgacaggagc gcgttcgcct
    23281 gcaactggtc gagcatgtcc tggcgttgat ccgtgtattg ctgcacgtcg taaggcgtag
    23341 aggcggcggc cagcgcgcgg ttggtgtcgt cgacctgtgc ctggagtcga tcggtttcag
    23401 cccggagcgc gagaagttct tgccgggcct ggtcgttctc cctgagcgcg gcaatggcct
    23461 gaatgacgtc ctgcggctca acttctcctg tgatgtccac acgaatcacg acttaatcct
    23521 gttccagcct ggtcgtcacc gtctggtcaa tcaccctgac gactccggcg gtgaagctac
    23581 ggatatcatc acgcgtcacg ttcaagtctt tcacttccgt gatgcgctcg acgtaggttg
    23641 ccacctgttc cagcgcgtct tgcttcgcgg cttccgtcgc gaggcgaacg gcatcggcgc
    23701 ggttgtcgtg atcacccata cggtattcac ccctggcggt gatcgtgcgc gcttcggccg
    23761 aacgcggacg aagttcggat gccatgtcag gtgcaggcgt ttccggacgt gccggtgtgt
    23821 cgaagctgcg ctgggtcagc tgcgggcggt atttctccgg gaccgatgcc aattcgttgg
    23881 taaaagtctg cacgccctcg tcgttcgtgt agctgtaaat ggtcgtcgag gcgaaagagg
    23941 gagccgtagg aagcacggag agccagagga cgaggagtgc ggcggtggtc ggtgtgctgc
    24001 gggagaagaa catggtacag cctcccgttc gtctacaggg tcaggataac acaaggaggg
    24061 caaccgattc tgctggccag ggcttagata tgttgggcga tacgagggcg ggtttgtcgt
    24121 gtggggaagt ccttctacag ggagtgtcca tagaagagcg ggatctcggg atttactggc
    24181 tcctcctcca gatcgcgcta ggatggaatc atcctatgct ggtcgtcaaa cgaatcatcg
    24241 ctgttgtgtt tgggctcgtc ccgcttgtgc tcattgcggc tggggtcgca atggttttcc
    24301 gggaactccg gcccactgat ccgttgttgt attccgtgca gcaggatggg gaactccccg
    24361 aggccttcat gtttgcggct gtggggtgcg ccggattatt cggctgccac cgtctgtggc
    24421 gagccgatgc cggctggaag tggaccctca tgccgattgc cgtgatggtg gctgcgatag
    24481 ccattcctaa tcttctctat ggacaccatg gatcgccgtc ggatcgagcc tactccaata
    24541 tgttcctgcg gctgggcacg ttcgccgttt cctcaaagca gctggcccag gaacaaggcc
    24601 agtttacctg tgatccctcc gtgaaactat tcgggctatc tcgtttcacc caaaacggtc
    24661 agcctcttcc ctatgtgatc ca
//
LOCUS       DAOQEW010000033        24659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_480_length_24659_cov_9.702528, whole genome shotgun sequence.
ACCESSION   DAOQEW010000033 DAOQEW010000000
VERSION     DAOQEW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24659
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_24659_cov_9.702528"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..387)
                     /locus_tag="PLS35_05585"
     CDS             complement(136..387)
                     /locus_tag="PLS35_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18161.1"
                     /translation="MTEALENGITRFWDWLDNRGVIRRLVLGVAIWMTWEVSAWSMWY
                     AVGNARPGLEIAAIIGAVTAPVAAFGGYVFKAYLDSRAT"
     gene            complement(384..908)
                     /locus_tag="PLS35_05590"
     CDS             complement(384..908)
                     /locus_tag="PLS35_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HNI18162.1"
                     /translation="MSRFDECLKFILAREGGYVDRSEDRGGPTNKGITQVVYSAYLQR
                     RHLPIQSVRFISDDEVADIYRSDYWKPCAGDNLPIPIDLVVFDGAVNHGVRQSIKFLQ
                     RALGTDDDGIIGPVTINAAHMDVNNGYKSHLVADVIDQRKDFYQRIVERDPSQRCFLN
                     GWSNRLKELGREVA"
     gene            complement(934..2649)
                     /locus_tag="PLS35_05595"
     CDS             complement(934..2649)
                     /locus_tag="PLS35_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNI18163.1"
                     /translation="MLAVELAGRLSKSIGAYGVAPQETGMVYRTLRDKVFDVVSPKDF
                     GAVGDGVANDTVAVLAACASGKLVDGGGRTYGMGGGVNQLSNFFVGMQNIRLKQLSPA
                     SAQTRTLYIASNNNRFVMRDVYIDRGGSAGYTVGDLADFAGIYIASSSLFTLDNVVVT
                     NGGRGNGIAIHNSTDFEISKCAVTEHYWQEVVPASPVIVDDIIQAFAINGCSRWSMNG
                     CLVKNVTSGSPGNYTTNVASTQYRRYTRLGVSGCDSFAISDCVSYNLDQCFDFSGSVG
                     NTNFAISNCIASMGGTYGFKLANSAAFGTVSGCLAADCGLYGFVISGPVGAVSQMPQD
                     IRFSGCIARNIGSNGIWSASSIAGFRIMSSAVIDPSYPRAIRFSGCHVVDSQGSPTCK
                     DGFVSDVSPLEYPTSGYDKAWANVVENCTVRGVISNHFNGIGPMALTVTGTTTQSISN
                     STWTAIDWATDVSDPCGMHSTSGNINIIYPKEAGYYSVSANVTFAANATGSRQLKFYV
                     SGAAVDTAIAKLPGDGTRTTTLNLTTTLFLNNGSNLAIYVHQDSGGALNIDRTQSSCT
                     VVKVH"
     gene            complement(2649..3059)
                     /locus_tag="PLS35_05600"
     CDS             complement(2649..3059)
                     /locus_tag="PLS35_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18164.1"
                     /translation="MAQNPLFAGTIKTPVAQIQNSDGTNAVTFFTAGASGSKLEAIAA
                     TSTDTSAVVVQAIVTVSAVDYVIGEINIPAGAGTNGTADSVRIMNITDLPWLCGDGVN
                     QVLLLASGVPLKFKAKTTVTAGKVLQFFGQGGDF"
     gene            complement(3062..3619)
                     /locus_tag="PLS35_05605"
     CDS             complement(3062..3619)
                     /locus_tag="PLS35_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18165.1"
                     /translation="MQPTAYTPATDFSEQEATGTSGRSTVNTAALDAELAAIEQTLDE
                     TLDNLALLQRDDGAVRNLTVHYEALAADVRLLLASGAFSADPTNAAWVTATAYAVGAF
                     VISSAVGYVCIVSHTSGASFAVDLAAGKWLQVAPVVFSASASSVGFTPTTTISASNAQ
                     AAIEEIDSELRTAANLFMAQNYGAL"
     gene            complement(3700..5397)
                     /locus_tag="PLS35_05610"
     CDS             complement(3700..5397)
                     /locus_tag="PLS35_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18166.1"
                     /translation="MSNPFDQFDAPAQKAGNPFDQFDAPRQPGKAPSGVLRGLRDPID
                     AAAQMLVHALPDSVVEAGNQANNWIAENTGLVGKLPEKNLSSLVTGQKTGIDGMLQQQ
                     EADYQAQRKAAGETGADWGRIGGNIAASLIPGAGLTRATQSMRLGPLAQAAVSGGATS
                     LLTPVLEGDSFIAEKAKQAAGGAVAGSIAQRAISGISRLVSPNASVNPDIQTLMNEGV
                     RITPFQALGGMAKRLEEKARSLPILGDAITAAHRRGGEDLNMAVLRRGIDNLNRAGMP
                     GQVTRGGGEGLQQLRTAAQDAYESLLPRMMADTGEQQFATNMANLRQMVQALPANEAR
                     YFDETLNREITQRLAPNGVLSGENLRAAQAALRDHAAQFLRSPDAYQRNLGAALRQAD
                     QELRGLIERANPQFSRQLGLINEAYRVMKTAQRAGSSVAAENGVFSPAQLHNAVKAMD
                     RTKDKRAFAEGTAFLQDLSGPGKRVLAQQYPDSGTAGRMFLGGGALASGLASPVIPGT
                     LLAGSAAYIPIIQRLLTGAVASRPQAAQGIAQQMQRVAPYLAAPGAVFGNAALQDVP"
     gene            complement(5397..6749)
                     /locus_tag="PLS35_05615"
     CDS             complement(5397..6749)
                     /locus_tag="PLS35_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18167.1"
                     /translation="MSDYGAMGTRIGRMFASGLNGDAYRNAYDNEALKLSRAGMAKAH
                     ADLYNEQAAQMRQRGMYQTPEFASQIAAALAGLSDGQGKQLDGFINSGSWGEVAGPAL
                     PIGQEGPPEPAQAPRPGWATPDAVSRYNTGRAVYLMNLGATGNSNAEQMAKAYADLVG
                     QGRIDAAMAKPDTIPTFGKAMAASQGKELFHQGANGVMDKFTGAESLNDVGKSAALEN
                     RAQAGNAAASAALHRAQIPEVQARIDLTRSKIAQPLILTDKDGNQTVYDPKAKADKPP
                     TEFQSKSATFGARAKEAHDVLDSLDGKYNVSAAMIGGGNGITAAIANPLLGNATQKAI
                     QAQRDFINAVLRQESGAVISDSEFANAAKQYFPQLGDSKEVIAQKRRNRETAIKGFTN
                     AAGKAAFEAPPIVPSGPNVLESVPEAAIAHLRSNPHLAPAFAAKYGKEATDAALRKGM
                     "
     gene            complement(6759..7418)
                     /locus_tag="PLS35_05620"
     CDS             complement(6759..7418)
                     /locus_tag="PLS35_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18168.1"
                     /translation="MCDVGTALSLGATLLGGVMQQQAASDAADAQREAIARAQDQQDQ
                     YARQAERTAMDNAKQYDPTQRAQRFDQARESAGDSLAQQLTAARESAPGLDQAQGRIS
                     KVFADASAKSGNQTTSEAMRDARLMGRMRGAQDMLTEEGYRNADYASQLGVIGRNAQG
                     AYRAAQPGITAARVPDSTQTLLGGLISGAGSALSNPQAMGRISSMFGSGFSRGSPTRP
                     F"
     gene            complement(7437..8045)
                     /locus_tag="PLS35_05625"
     CDS             complement(7437..8045)
                     /locus_tag="PLS35_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18169.1"
                     /translation="MGIPVYGSGATYSPIYENIMTEKPTAAAAAADARARMYDTVRGD
                     TRNFYSQQLNDDREKAAREMRFRLARAGTAGSSQAIDMGRDFTKANDRGLLDVANRAD
                     SAASNVRSSDEQSRLGLISKIVAGLDQGSAMSSALSQLQTNADIERQNSMAGRMENMF
                     ADLFGTYNNGQIASGVSDARRAYGGGLGNYFGNGRSENGRIS"
     gene            complement(8296..8718)
                     /locus_tag="PLS35_05630"
     CDS             complement(8296..8718)
                     /locus_tag="PLS35_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18170.1"
                     /translation="MRGSDREEVMATRWSDSPYDFAADCMRAPGSRFTAIYEGRPVML
                     GGVAMNQPGIGQAWMVGTDEIGNLGVEIAHASKAIFSRLLEGHVHRIQAYSIATHQWA
                     HSWLRKIGFVEAASLKQFGKRGEDFVLFEMLKESADVQ"
     gene            complement(8763..10706)
                     /locus_tag="PLS35_05635"
     CDS             complement(8763..10706)
                     /locus_tag="PLS35_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18171.1"
                     /translation="MPVITFDNFANGLDLRKGASVSDANRLRGLDNCYVTTGKTIRKR
                     PGIIQEATIEAGTTGLRAAGGILNTFYASGSITHANPLFEAHKVTNPNDPATSIEKVW
                     YADSFNGFLYVVVEYDNGDVVHHYLDDTSALTPWSASAALTAGGEIRPAVDNGLRYEI
                     TTGGVTANTAPAFPTTINATVTESESNYTAWTASSLKTLGKAYKPTVSNGYVFKATNA
                     GASAGTEPTWPTTVGATVTEDDTTATAWAATTALSLGAIRRPTVANTYLYEVTTAGTT
                     AGAQPTWPTTVGATVTDGSVTWTCRSRAAYECTTKPAYKCHGTEIYDPNCPQSKAVVK
                     KASKIFAIGDEVVPFCKTNDPRNWTEPADAGFLGVGIQQSGAVNPTALGEYAGNLVVF
                     FKDSAQVWQVDPDPANMKFVQGIDVGCPYPYGAANMAGDVFFASFDGVRSITTQATTG
                     SLIDVDVGSPIDSLVSQVLTPSASVRAFYYRGGGQFWVLVDSTAYVYSFSRTSKISAW
                     SRYTFNFQIDDVSELEGDLYIRSGDSVYRMDRDATSDAGEVFPLSIEFAFLDFQAPGV
                     LKQVHGMDAVMVGSAEISHKYDAKNPDYQTASAGFDGDSRPGGLSPVELCSVGIAPVI
                     TNAKDEEFELHALTYYFDPLGAL"
     gene            complement(10709..11347)
                     /locus_tag="PLS35_05640"
     CDS             complement(10709..11347)
                     /locus_tag="PLS35_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18172.1"
                     /translation="MTYKTLGELRSNLAVRLGFGAAGSSGVNAPILDMFLQNAQDQLY
                     AQFDWRHLVKYDEKATETGSFLYDWATDCNQDRILDIAIHDGSRFVPMKEGISWAMRS
                     DDSQTIPMRYERFQQMEIWPIPDAAYTMRRYYVQSLGRFTQDNDRASLDDDLIFLHAL
                     ANAKAHYRHPDAQTYSSQLTAMMDKLKERSRGKHVHRRDDPDDVYLARPRDV"
     gene            complement(11344..11676)
                     /locus_tag="PLS35_05645"
     CDS             complement(11344..11676)
                     /locus_tag="PLS35_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18173.1"
                     /translation="MLAKHVLVVVSRDMAEKLPATVFEHEVELLKDIHGDGSVDIVDR
                     LEAADPIEVDADMEFDRLISYYGTNDNGQPYAERVFGRSSRGLEAHAFRPAKRGRKAA
                     EEAQPEAE"
     gene            complement(11726..12067)
                     /locus_tag="PLS35_05650"
     CDS             complement(11726..12067)
                     /locus_tag="PLS35_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18174.1"
                     /translation="MSIKSVSLGTLTSATAGTSQKAEDSDAAFQRGGYVYAVIQPTNG
                     AFVGTAKIQGTNDLDTVVDGSATWTDLLTFTAPGSNSGSKSAVVTAYRRMRLNVTAFT
                     SGSVDGLLIGA"
     gene            complement(12080..13147)
                     /locus_tag="PLS35_05655"
     CDS             complement(12080..13147)
                     /locus_tag="PLS35_05655"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNI18175.1"
                     /translation="MPFSTQELQDAGKVAIDFYLKNNPIDQVAVERPFLKAMMAKKKS
                     FPGGKQYIIEQLRYRYQSNFQWFNGASVVTYNKRVNIEQSQFPWRSAHDGFSLDEDRL
                     AQNGISIDDSGGGGNASAAERVQLTSLLQDQVETLRLGFEEKFSMYLQLDGTSSTDAV
                     AGLDALVSTTPTTGTVGGIDRSSSSNVWWRNSVTLNITAVTTQATALSMLGTMETMWR
                     ACVKNGGRPDLIFAGATFIDNYIAALGYSGQQIQYAGGEPRKLDGGVSGVYYKGVEIQ
                     WCPEFDDNFGGFVSPTPSWTKRCYFINSRTITLRPMDGQDMVTRKPPRVYDKYVYYWA
                     LTWRGALTTNRSNANAVMAMA"
     gene            complement(13354..14349)
                     /locus_tag="PLS35_05660"
     CDS             complement(13354..14349)
                     /locus_tag="PLS35_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18176.1"
                     /translation="MEKVELQSPEVTQPEAIEPVEQQEPSTLLDAINAELDGKTDDGV
                     SRDEKGRFAKKEETPTSETKPEEQKPEVQKAEAKPADETEMPEGLSSKAQDRFRNLVT
                     RLHEKDDQIAQANEVIGEFRQMIQSTGATAEEFSRAIDYMTRIKSGDLEGALTMLDQQ
                     RQMIAFALGKPLPGADPLAQYPDLRQAVEAYRMDEQAAMEIARAREFQRQQTSAHERQ
                     QQATQAQHNEMAQRQQAVTAIDQMTAQWAKTDPDFTAKEELILKQLPEIASNFPPSMW
                     QQQVRILYNTISAMPMARPAPPTPAPLRASGQSAGAKQPASMLEALEGALGYSAG"
     gene            complement(14360..15055)
                     /locus_tag="PLS35_05665"
     CDS             complement(14360..15055)
                     /locus_tag="PLS35_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18177.1"
                     /translation="MLPFAPISGLPVSGQPVSSGGEITLVGANSTQANTSGTGAITQT
                     HVLTGTNSAQDNTSGTGSISQVHILSGANSTQDNISSTGAISLGSSIDLIGANSAQDN
                     VSGTGAISQTHNLTGSDSSQSNTSGTGAIAQVHILSGADCTQINLSPDVSFITGPLFA
                     SLGGTPDKPRKPVKAKRKKPQEEIESPLPPETAGRIRDELLGASLTADVIERAKARAK
                     RIQAEETAILLIL"
     gene            complement(15055..15456)
                     /locus_tag="PLS35_05670"
     CDS             complement(15055..15456)
                     /locus_tag="PLS35_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18178.1"
                     /translation="MINYDEETNQYVNCVDPDVSIADAVNLVSWLIETHASRLRDRYI
                     KPVSPYEAASWPLKLAEAKAGGGPMLQAEADARGVDLSVIVEKVMDKAQALAGLEAMI
                     AGVSGKHRDAISAKEDAHSVLDYDWSIGWPE"
     gene            complement(15410..15862)
                     /locus_tag="PLS35_05675"
     CDS             complement(15410..15862)
                     /locus_tag="PLS35_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18179.1"
                     /translation="MLPVLPVQGAPLPGAVVRGPGAVVEAGQAQTVARAVTLQVRLVI
                     QPLRAERQAVRQTQGTAPTGIIYSTHRHFILSHSVARVAVARLAALGLQAGTVGMEVL
                     DAAEGEAEIRVRAEPPATAAKAVTESPFSSGCDHDQLRRRNEPVRQLC"
     gene            complement(16373..16813)
                     /locus_tag="PLS35_05680"
     CDS             complement(16373..16813)
                     /locus_tag="PLS35_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18180.1"
                     /translation="MAANQDPIFCGTPDIQSGGGAVIGPTANTNQDGTGTVYSVWQAD
                     ATNGGFCQKLMAKSVGSPAATAFRVYISSITGTFTGNTAANTWLIKDVSLPTTTLSQT
                     AGSLDVEVPINLPIPPGYRVCCSFGTSTGAAGTGWMVTGVGGKY"
     gene            complement(16817..17164)
                     /locus_tag="PLS35_05685"
     CDS             complement(16817..17164)
                     /locus_tag="PLS35_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18181.1"
                     /translation="MPSIADRVWETNSATGTSDFVLTGAKTGYQTFAAGCGSAAVIVP
                     YCAVSGSEWECGYGTFNGTTGITRDKIESSSNSNAEVSFSSAPDVFLTLIADLSQNAR
                     LGRQLARLIFPTW"
     gene            complement(17164..17547)
                     /locus_tag="PLS35_05690"
     CDS             complement(17164..17547)
                     /locus_tag="PLS35_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18182.1"
                     /translation="MAKWAHADVLDNGINHIKNNATKMMVISAYTAGDSYATVTGNNC
                     AEVTMTGTDYTVSSSGNNRQVQSAAGKSATATANSGATPNLHIAFTDGSAKVLWVTDE
                     TSDQVITSGNTVNFPQLTYTSNQPT"
     gene            complement(17557..19587)
                     /locus_tag="PLS35_05695"
     CDS             complement(17557..19587)
                     /locus_tag="PLS35_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18183.1"
                     /translation="MQAVLNRKFIRDARLKKRAKASIRAAMTVEIGWAKVTWQKDISR
                     DPLIESRIADVQDNLQRIQYLRDELAEGDGAKADLDAKEGELKQQIAALNANVEVTRF
                     SGIVVDHVLTEDIFILDETMYEFDGYENAGAIAHRVWMTTEQYEQQFGKEPPKTATRF
                     GQDKKTQGSNNNDAPLLVAVFETWDRASNTIYTLCAGADQWAREPYQPTLSGKRFYPF
                     FGLAFNPIDGSVVPISDAALLIELQDEYNTTRTNFSEHRKENLPVRVFRNSGDLSDAD
                     VRNLKNRASNEWIGIGGDPNKPITNDVAVLPNPPVDPMTYDVAPILRDAELVLGAGDA
                     SKGTINKAKTATEAEIMAQGLQTRMAERQDVVEDWISEMAQYAAELCLQEMTLEDVQR
                     IAGKGAVWPQMTKDQVFDLVQIEIRAGSTGKPNRAKEREQWVQMLPQIKEAVTQVAQL
                     RAAGQDDMATTIIKLMEETLRRFDERLDIESFIPPAKAHEPDGQQPPQIPAEIQQALQ
                     QIPAMQDALQQLQAANQQLQAQLADKSQAMGLERDKFEYQKTADLADNEARIAEAQIK
                     ADADAAAKIEIERIRSASKGEEIASRERVEIEKARKAQEQAAMQQPVAQGAEPQVIEA
                     VCQMHEALCAMSKQFADQMAALQAHLTAPKRVIRDPQTGEMIGVETVPHTLN"
     gene            complement(19883..20203)
                     /locus_tag="PLS35_05700"
     CDS             complement(19883..20203)
                     /locus_tag="PLS35_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18184.1"
                     /translation="MNEISRVCQGLSVSRPSLDHQQLLALIDPSPEFEAHIPAETGKD
                     AWIYTVNGKTLEGECIVVRAIWPWQAETLAQEGLADTIAALRKLDQATGLSVSAEAKP
                     YGAH"
     gene            complement(20264..21703)
                     /locus_tag="PLS35_05705"
     CDS             complement(20264..21703)
                     /locus_tag="PLS35_05705"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNI18185.1"
                     /translation="MWDPQPGAQTSFLLADWCDEIFYGGERGGGKSDAQCGYQEDGAL
                     RYRDKWRGIMFRKTYPELEELQARASEIFPGEGAVYKSQSSAEHPFSNCWYWPSGATV
                     KMRYIETEKDYGRYHGHQYTGISFDEVTEYHTPSGLLKMLSTLRSAFGVPCTMRATGN
                     PGGVGHGWVKQRYIENVPPYHPFKDPDTGFIRMFIPSRTSDNQILLRADPHYQQRIRA
                     ATGGNEVLRKAWLEGDWNIVAGAFFSEWNSSRHVIKPFSIPIHWTRYRAFDWGSAKPF
                     ACYWIAVSDGSMPAFPRGALIVYREYYGMQSGHPNVGLKMTADAVAREIIRMDGKETT
                     HDTGWGVADPAIFTEDGGPSIAEMMRREGLRWRRADNKRRPGWEQMRIRLSCDDDGLP
                     LLVIFDTCVHLIRTLPTLQHDDHNPEDIDTDQEDHAGDALRYGVMARPIIRSERPKED
                     GPRPGTAEHMMMITDAPKEISKYRSIRRK"
     gene            complement(21703..22017)
                     /locus_tag="PLS35_05710"
     CDS             complement(21703..22017)
                     /locus_tag="PLS35_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18186.1"
                     /translation="MKTRTSGQGRKKGAINKVTREVREVVARVAESTAPEIEQWLRDV
                     ANDDPGRALDLWLKMIEYHIPKLARTEMTGEGGGAVKYEHDVLVRPVMSREEWLKNHG
                     IV"
     gene            complement(22401..22799)
                     /locus_tag="PLS35_05715"
     CDS             complement(22401..22799)
                     /locus_tag="PLS35_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18187.1"
                     /translation="MGNDLQDHWRGYRVWDAGVQRMSGKLSRNKGARGQSEFKAMLLD
                     RDWTVDTLTAGIKSADLIAKDPQGMEWCIEVKNCAGILPAHRKQAMEQGDKRRLPWML
                     ASKIAGSSSWLVQRRGVQPVVWTTKGAESS"
     gene            complement(23017..23400)
                     /locus_tag="PLS35_05720"
     CDS             complement(23017..23400)
                     /locus_tag="PLS35_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HNI18188.1"
                     /translation="MSKVLILNSDVARQAACRYVMAAPNGYAVKISERTRSLDQNAAQ
                     WPILDAFAEQLQWPVNGAMQTITAEDWKDILTCAWRNEVPRVAMGLSGGMVLLGQRTS
                     RFKRGEFSEWLDWLNAMAVEKGVQL"
     gene            complement(23393..23572)
                     /locus_tag="PLS35_05725"
     CDS             complement(23393..23572)
                     /locus_tag="PLS35_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18189.1"
                     /translation="MTIPLKINLKAEANFCRWCVHAAWSVQSPEHMWCGAWVRFVPRN
                     ATCEHYMREPGADDE"
     gene            complement(23851..>24659)
                     /locus_tag="PLS35_05730"
     CDS             complement(23851..>24659)
                     /locus_tag="PLS35_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI18190.1"
                     /translation="RFRFRQAEVTVWTGYNGHGKSYALGMVCASLVSQRSKVCIASFE
                     MPARRTLYRLIRQAAGHDTLDAEFARGYLANVKEHLWLYDHLGQTTPEKLLAVIRYCS
                     DKKGIQHFVIDSLLTCGLPEDGNGALTAQKQFIGDLCVIARDTGIHIHLVAHARKGAT
                     DEDEGKTPGKFNVRGSSAITDQADNVISVWRNVRKERAFDPNGSLGGKPVSSDEKVSL
                     SMQADAMLAIAKQRNGEWEGKIGLWLDRESWQFVENDQGILVDLLCFDGP"
BASE COUNT         4864 a         7560 c         6654 g         5581 t
ORIGIN      
        1 gatactcggc gcgaacctcg gcccggcctt cctcgcgcac gctctcgagg aacagatgca
       61 caccccatcc gatggcagct acgaggacca ggccagcaag cccacggacg agataaggga
      121 ccattgcggc tgggatcatg tcgccctcga atcgaggtag gctttgaaga cgtaccctcc
      181 gaacgccgcg acaggagccg tgacagcccc gatgatggcc gcaatctcca gtccaggtcg
      241 tgcgttcccc accgcgtacc acatggacca tgctgacacc tcccaggtca tccagatggc
      301 taccccaagg acaaggcgac ggatgacccc ccggttatca agccagtccc agaatcgcgt
      361 aatcccgttt tcaagcgctt cggtcatgca acctcccgtc caagctcttt caacctgttg
      421 gaccatccgt tgagaaaaca cctttgcgac gggtcgcgtt ccacaatccg ctggtagaag
      481 tctttccgct gatcgataac atcggcaacc agatggctct tgtaaccgtt attgacgtcc
      541 atatgcgcgg cattgattgt gaccggacca ataatcccgt cgtcgtccgt ccctaatgcc
      601 cgctgtagga acttgatgga ctgacggacg ccatgattga ccgcgccatc aaatacaacg
      661 agatcgatag ggataggcaa attatcgcca gcgcaaggct tccaataatc ggatcggtag
      721 atgtctgcaa cctcatcgtc tgagatgaat cgtacgcttt gaatcggtaa atgtcggcgc
      781 tgaagatagg ccgaatagac cacctgagtg attcccttgt ttgtcgggcc gccccggtct
      841 tcacttcgat caacataacc gccctcccga gcaagtataa atttcagaca ttcatcaaat
      901 cggctcatgt catcccctct tgcctgacgt tcatcagtgg accttcacga cagtacaaga
      961 tgattgagtt ctatcgatat tcaacgcccc gccagaatct tgatggacat aaatagccaa
     1021 gttcgatcca ttattcagga aaagggttgt tgtcaggttt aacgtcgtgg tcctggttcc
     1081 atcgccaggc agtttggcaa tcgccgtatc aacagccgcc ccgctaacgt agaatttcaa
     1141 ttgccgcgac cctgtcgcat tggcagcgaa cgtgacattc gctgaaacgc tgtaataacc
     1201 ggcttccttg ggataaataa tattgatatt tccggacgtc gaatgcatcc cgcatgggtc
     1261 tgaaacatcg gtcgcccaat caatggccgt ccaggttgaa ttgctgattg attgcgttgt
     1321 tgtccctgtt acggtcaagg ccatcggccc gatgccgttg aagtgattgg agatcacccc
     1381 gcgaaccgtg cagttttcaa ccacgttcgc ccaagccttg tcatacccgc tcgttgggta
     1441 ttccagaggg gaaacgtcgc tgacgaaacc gtccttgcaa gttggagagc cctgcgaatc
     1501 aaccacatga cacccagaaa acctaattgc tctcggataa cttggatcga taaccgccga
     1561 ggacatgatc cggaaccccg caatagaaga tgccgaccat attccgtttg atccgatatt
     1621 gcgggctata caccctgaaa accggatgtc ctgcggcatt tgggaaaccg cgccaaccgg
     1681 acccgagatc acaaacccat aaagcccaca atcagccgca aggcacccgg ataccgttcc
     1741 aaatgccgca gaattggcga gcttgaaccc ataggttccg cccatagagg cgatgcagtt
     1801 ggatattgca aaattcgtat tcccaacgct cccagaaaaa tcaaaacact gatccaggtt
     1861 gtaagaaaca caatcagaaa tcgcaaaaga atcgcacccg cttacaccaa gtctggtgta
     1921 acggcgatat tgcgttgagg caacatttgt tgtgtaattc cccggagaac cggatgtaac
     1981 gttcttgaca aggcacccat tcatcgacca ccgagaacac ccgttaatcg caaaagcctg
     2041 aatgatgtca tctacgatga ccggggacgc ggggacaacc tcttgccaat aatgctcggt
     2101 gaccgcgcac tttgaaatct cgaaatcggt tgaattgtgg atagcaatcc cattcccccg
     2161 gccgccgttc gtaacgacga cattatccag cgtgaaaaga ctggatgacg caatatagat
     2221 tccagcaaaa tcggcaaggt cgcccaccgt gtaaccagcc gatcccccgc ggtcgatata
     2281 gacatcgcgc ataacaaaac ggttgttatt gctcgcgatg tagagcgttc gggtctgagc
     2341 cgaggccggg gagagttgtt taagccgaat gttttgcatg ccgacaaaaa aattcgacaa
     2401 ttgattgact ccccctccca tgccataggt ccgcccgccg ccgtcgacta atttgccgga
     2461 tgcgcacgcg gccaatacgg caactgtatc gtttgcgaca ccatcgccaa cggctccgaa
     2521 atccttgggg cttacgacgt caaaaacctt atccctgagc gtcctataga ccatcccagt
     2581 ctcttgtgga gcgacgccat aagccccaat ggacttggaa agcctccccg ccaattcaac
     2641 ggccagcatt agaagtctcc gccctgaccg aagaactgaa gcaccttgcc cgccgtaacc
     2701 gttgttttgg ccttaaactt cagcgggact ccggatgcca acagaaggac ttgattaacg
     2761 ccatctccac acaaccaggg aaggtcggtg atattcatga ttcgcacaga atcggccgtt
     2821 ccatttgtcc ctgccccggc cggaatgttg atttctccga tgacataatc aaccgcgcta
     2881 accgtaacga ttgcctggac cactaccgcg cttgtatcgg tgctggtggc cgctatcgcc
     2941 tctagcttcg atccactcgc gccagccgtg aaaaacgtta cagcattcgt gccgtcagag
     3001 ttctgaatct gagcaaccgg agttttgatg gtgccggcaa atagggggtt ctgggccatg
     3061 attaaagcgc tccgtagttt tgagccatga aaagatttgc cgcggtgcgc aactcgctat
     3121 caatttcctc gatagccgcc tgcgcatttg aggcggatat ggttgtggtt ggggtaaatc
     3181 cgacgcttga cgcggatgct gaaaaaacga cgggagcgac ctggagccat ttcccggccg
     3241 ccagatcaac ggcgaacgac gccccggagg tatgggacac gatgcaaacg tagcccaccg
     3301 cagaagaaat gacgaacgcc cccaccgcat aagccgttgc cgtcacccag gccgcattag
     3361 tcgggtccgc tgaaaaagca ccggaggcga gaagcaatcg cacatcggcg gccaaagcct
     3421 cataatgcac ggtgagattt ctgacggccc cgtcatcccg ttgcaagagc gccaggttgt
     3481 ctagagtctc gtctagcgtc tgctcgattg cggccagttc agcatccagc gccgccgtgt
     3541 tgacagtgct tctcccggat gttccggtcg cttcctgttc gctgaaatcg gttgccgggg
     3601 tgtaggctgt tggttgcata cttttttcct ggtcgggcct ctatggatga ttcgtggcgt
     3661 ccttacatcg tttcttttgc agtcgttttc atcatcgggt tatgggacat cctgaagcgc
     3721 agcatttccg aatacggcgc ccggagcggc aaggtagggt gcgacacgct gcatttgctg
     3781 ggctatgcct tgggccgcct gtgggcgaga tgcaaccgcc cccgtcaata gccgctgaat
     3841 gatcggtatg taggccgccg atcccgccag cagcgtccca ggaatgaccg gagaagcaag
     3901 tccggatgca agggcgccgc ccccgaggaa cattcgtccc gccgtcccgg aatctggata
     3961 ctgttgcgca aggacacgtt tccctgggcc tgaaaggtct tgcaggaaag ccgtcccctc
     4021 agcgaatgcg cgcttatcct tcgtcctatc catcgccttg acggcgttat gaagttgcgc
     4081 cggggaaaag acgccatttt cagcagcaac ggaagacccc gcccgctgcg ccgtcttcat
     4141 cacccgatat gcctcattga ttaggccaag ctgacgggaa aactgcggat tcgctcgttc
     4201 gatcaatccg cgcaattctt gatccgcctg cctcagagct gcccctagat tgcgctggta
     4261 ggcatccgga gagcgcagga attgcgccgc gtgatcccga agtgcggcct gtgcggctct
     4321 caagttctcg cccgatagga caccatttgg ggcaagcctt tgggtgatct ctcggttcag
     4381 cgtttcgtcg aaatatcggg cttcgttagc gggaagcgcc tgaaccatct ggcgaaggtt
     4441 cgccatgttc gtagcaaact gctgttcccc ggtatctgcc atcatgcgcg gcaacaagga
     4501 ctcgtaagcg tcctgcgctg ctgtcctcag ttgttgcaat ccttccccgc cgcctcgcgt
     4561 gacctgtcca ggcatcccgg ctcgattgag gttatcaatc ccgcgccgga ggacggccat
     4621 attcaagtct tccccgcccc tgcgatgtgc tgccgtgatg gcatccccaa ggattggaag
     4681 gcttcgcgcc ttttcctcca ggcgtttggc catcccgccg agggcttgaa acggggtaat
     4741 ccgcacgccc tcattcatca gcgtttgaat atcaggattg accgatgcgt tagggctgac
     4801 cagcctggaa attcctgaaa ttgccctctg agcaatcgat cccgcaacgg ccccgccagc
     4861 agcctgtttc gctttttcag cgataaagct gtcgccctca agaaccgggg tcagcaaaga
     4921 ggttgccccg cctgaaacgg ccgcctgcgc caatgggcca agcctcatgg actgagtggc
     4981 ccgtgtcaac ccagccccgg gaatcagtga cgccgcaata ttcccgccga tcctgcccca
     5041 gtcagccccc gtttctcctg cagccttgcg ttgcgcttga tagtccgctt cttgctgctg
     5101 cagcatccca tcaatgcccg tcttttggcc ggtcactagc gaagacaggt tcttctccgg
     5161 taacttcccg acaagtccgg tattttccgc aatccagttg ttcgcctggt tccccgcctc
     5221 gaccaccgaa tccggcaaag catggacaag catctgcgcc gccgcgtcga ttggatctcg
     5281 gagcccgcgc agaaccccgc taggcgcttt cccaggttgg cgcggtgcat cgaactggtc
     5341 gaacggattt ccggccttct gggctggcgc gtcgaattgg tcgaagggat tcgacattac
     5401 atgcccttcc ttagcgccgc atcggtggct tccttgccat acttggcggc aaaggcaggc
     5461 gcgaggtgtg gattgctccg aaggtgagca atggctgcct cgggaacgct ctccaaaacg
     5521 ttcggcccac ttggcacaat cggaggcgct tcaaatgcgg ctttgccagc ggcattcgtg
     5581 aatcccttga ttgccgtttc cctgtttctg cgcttctgcg caataacctc tttggagtct
     5641 cctagctggg ggaaatactg ctttgccgcg ttggcgaact cagaatccga aatgaccgcc
     5701 ccggattcct gacgcagcac ggcattgatg aaatcccgct gcgcctgaat ggctttttgg
     5761 gttgcattcc ccaagagggg attcgcaatc gcggcagtaa tcccgtttcc gccgccgatc
     5821 atggccgctg agacgttata tttaccgtcc agggagtcca aaacatcgtg cgcctccttc
     5881 gctctggcgc cgaatgtcgc tgatttcgac tgaaactctg tcggcggctt gtccgctttt
     5941 gccttcgggt cataaaccgt ctggttcccg tctttgtcag tcaggatcag cggttgggcg
     6001 atctttgaac gggtcaggtc aattcttgcc tggacctctg gaatctgcgc ccgatgcagc
     6061 gcagcggaag ccgcagcgtt tccggcttgt gcccgattct caagcgcggc ggatttgccg
     6121 acatcattga gcgattcggc cccggtgaat ttgtccatga ccccgtttgc gccttggtgg
     6181 aaaagttctt tcccctggct cgcggccatg gccttgccga aagtcggaat cgtgtcaggc
     6241 tttgccattg ccgcatcgat ccgcccttgt ccgaccagat cggcgtaagc cttcgccatt
     6301 tgttcggcgt tgctgttccc ggtcgcccca aggttcatga gatacaccgc cctgcccgtg
     6361 ttataccgag agacagcgtc aggggtcgcc catcccgggc gcggggcttg cgccggctcc
     6421 ggagggcctt cctgcccgat cggcaacgcc ggcccggcaa cttcgcccca ggagccggag
     6481 tttatgaagc cgtccaactg cttcccctgc ccgtcactca gccccgccag ggctgccgca
     6541 atctggctgg cgaactcagg cgtctgatac atgccgcgct gacgcatctg cgccgcctgt
     6601 tcgttgtaca agtcagcatg cgcttttgcc atcccggccc gggagagctt gagtgcttcg
     6661 ttgtcataag cattccggta agcatcccca ttcaagccgg atgcgaacat ccgccctatc
     6721 ctggtcccca tcgcgccgta atcgctcata ccggcctctt aaaatggacg agtcggggag
     6781 ccgcgggaaa acccgcttcc gaacatggaa cttatccgcc ccatggcctg cggattgctt
     6841 agggcgcttc cagccccgga gatgagcccg ccaagaaggg tttgcgtgct gtcaggaacc
     6901 ctggcggctg taatcccggg ctgtgctgcc ctataggcgc cttgcgcgtt tctgccaatc
     6961 acgcctagtt gggatgcgta atccgcattc cgatagccct cttcggtcag catgtcttgc
     7021 gccccgcgca tccgccccat aaggcgggcg tcccgcatgg cttccgatgt cgtctgattg
     7081 ccggattttg cggatgcgtc agcaaagacc tttgaaatcc tcccctgcgc ctggtcaagc
     7141 cctggtgcgg attcgcgagc agccgtcaat tgctgggcca acgagtctcc ggcagactcc
     7201 cgcgcctgat cgaagcgttg cgcccgctgc gtcgggtcgt attgcttggc gttatccatc
     7261 gccgtgcgtt cggcttggcg agcgtactgg tcttgctggt cctgagcgcg tgcgatggct
     7321 tcgcgctgcg cgtcagcggc atcagaggcc gcttgctgct gcatcacgcc gcccagcaag
     7381 gtggccccca gagatagagc ggttccgaca tcacacatat ctatttcctc cggcggttag
     7441 gaaatccgcc cgttttcgct tcgtccgttg ccaaagtaat tcccgagtcc gccgccataa
     7501 gcccttctgg cgtcgctcac tccggatgca atctggccgt tgttgtatgt cccgaacagg
     7561 tcagcaaaca tgttttccat gcgcccggcc atggagttct ggcgctcaat gtcggcattc
     7621 gtctgcagtt gagagagcgc cgaactcatc gcgctgccct gatccaggcc ggcgacgatc
     7681 tttgaaatca gcccgagccg ggattgctca tcggaacttc gcacattcga cgcggcggaa
     7741 tccgcacgat tggcaacatc cagaagcccc cggtcattgg ccttggtgaa atcccgcccc
     7801 atgtcgatag cttggcttga tccggccgtc cccgcccgag cgaggcggaa ccgcatctca
     7861 cgcgccgctt tttcgcggtc gtcgttcaac tgctgcgagt aaaagttgcg ggtatcccca
     7921 cgtaccgtgt catacatcct ggcgcgtgca tcggccgccg ctgcggctgc ggtcggcttt
     7981 tcggtcatga tgttttcgta gatcggggaa taggtcgcgc cgctgccata gaccgggatt
     8041 cccatgacgc gtcccgtcat cgtccgttgc gccgtctcgt actcttgagg ggttacggtg
     8101 cgtgacgttg cgggcgtgaa ttcatcgccc ggggttgtca tcgtgtaacc tgtcaatcgc
     8161 ttttccccgg tcggggacat ggcagtcctt gggccaatcc cgaaaagctc attcacggcc
     8221 tttgttgccg catcaatccg cgcctgccga gcggcttccc gctcagatat tccgccatcg
     8281 cctccgcctc cgccgctatt gcacatcagc gctctccttc agcatctcga acaaaacaaa
     8341 atcctcgccg cgcttcccga actgtttgag actggccgcc tcaacaaatc cgatcttccg
     8401 cagccatgaa tgcgcccatt gatgagtggc aatcgaatac gcctggatgc ggtgcacatg
     8461 gccttccaga agccgggaga agatggcttt cgatgcgtgc gcgatctcaa cgccaagatt
     8521 cccgatctcg tcggttccca ccatccaggc ttgcccgatt ccaggctgat tcattgccac
     8581 cccgcccaac atgaccggac ggccttcgta aattgccgtg aagcgcgatc caggcgcccg
     8641 catgcaatcc gcggcaaagt cataagggct gtcgctccac cgcgtcgcca tgacctcttc
     8701 ccggtcggag ccacgcatgt tcaaacatac ttccagagcg tctttccagg tcgcttccac
     8761 gatcacagcg cccccaatgg gtcaaagtaa taagtcagtg cgtgcaactc gaattcttcg
     8821 tccttcgcat tggtgatcac cggggcgatc ccgaccgaac acaactcgac aggggacagc
     8881 ccgccaggac gtgaatcccc atcaaagccc gctgatgctg tctgataatc cgggttcttg
     8941 gcgtcgtact tgtgagagat ttccgcgctc cccaccatga ccgcatccat gccatgaacc
     9001 tgtttaagca cgccaggagc ctggaaatcg agaaacgcaa actcgatgga gagcggaaag
     9061 acctctccgg cgtccgaagt agcgtcacgg tccatccgat agaccgaatc ccctgaacgg
     9121 atatacaaat cgccttccag ctcggagacg tcgtcaatct ggaaattgaa cgtgtaacgg
     9181 ctccatgcag aaatcttcga tgtacgcgaa aaggaataaa cgtaggctgt cgaatcgacg
     9241 agaacccaga attgaccgcc gccccggtaa taaaaagccc gaacagaagc agaaggggtc
     9301 agcacttgcg aaacaaggga gtcaatgggc gagcccacat caacatcaat caggctgccg
     9361 gttgtcgctt gtgtcgtgat ggagcgcacc ccatcaaagg acgcgaaaaa gacatcaccg
     9421 gccatattgg ccgccccata ggggtacgga cagccaacat caatcccctg aacgaatttc
     9481 atattcgccg ggtccggatc gacctgccac acctgggcag agtccttgaa gaagaccacg
     9541 aggttgccgg catactcccc gagcgcagta ggattcaccg ccccggattg ctgaatgccg
     9601 acgccaagga atccagcgtc cgccggctcc gtccagtttc gcgggtcgtt tgtcttgcag
     9661 aacgggacga cctcatcccc gatggcaaaa atcttgcttg cctttttcac aacggctttt
     9721 gactgcgggc agttcgggtc gtaaatctct gtcccgtggc acttgtaagc gggctttgtc
     9781 gtgcattcgt aagccgccct ggaccgacaa gtccatgtca ctgagccatc cgtcacggtt
     9841 gcgccgaccg tcgtcggcca tgtcggttgc gcccccgccg ttgtccctgc ggtcgtgact
     9901 tcgtaaagat aggtattcgc cacggtcggg cgacgaatcg cgccgaggga aagcgccgtc
     9961 gttgcggccc atgccgttgc ggtcgtatcg tcctcggtga cagtggcccc gaccgtcgtc
    10021 ggccaggtcg gctccgtccc ggcggatgcc ccagcgttcg tggccttgaa aacgtagcca
    10081 ttgcttacgg ttggcttgta cgccttccca agagtcttga gcgaagacgc cgtccaggcc
    10141 gtgtaattcg attcgctctc agtgaccgtg gcgttaatcg tggtcgggaa cgccggggct
    10201 gtgtttgcag ttaccccgcc ggttgtgatc tcgtaccgca atccgttatc tacggccggc
    10261 ctgatctcgc cgccagccgt cagcgcagcg gatgcgctcc acggcgtcag cgcagaagtg
    10321 tcgtccaggt agtgatgcac gacatcgccg ttgtcgtatt cgaccacgac atacaaaaac
    10381 ccgttaaagc tgtcggcgta ccagactttt tcaatggatg tcgcggggtc gttcggattt
    10441 gtgaccttgt gcgcttcaaa caaagggttt gcgtgcgtga tcgatccaga ggcatagaag
    10501 gtattcaaga ttccgccggc agcgcgcagc cccgttgtcc cggcctctat cgtggcttcc
    10561 tggatgatcc cagggcgctt gcggatcgtc ttgccggtcg tgacatagca gttgtccaac
    10621 ccccgcaacc gattggcgtc ggaaaccgac gcgcctttcc gcaaatcaag cccgttggcg
    10681 aaattgtcaa atgtgataac cggcatggct atacatcccg aggccgcgcc agataaacat
    10741 catcgggatc gtctcgacga tggacatgct tcccgcggct gcgctccttg agcttgtcca
    10801 tcatggccgt caattgactg ctgtaggttt gcgcgtcagg gtgccgatag tgcgctttgg
    10861 cgttcgcgag ggcatggaga aagatcaggt catcatccag gctcgcccgg tcgttgtcct
    10921 gggtgaatct tcccagcgat tgaacgtagt agcggcgcat ggtgtaggcc gcatcgggga
    10981 tcggccagat ttccatctgc tggaaccgct cgtaccgcat ggggatggtt tgggaatcgt
    11041 ccgagcgcat cgcccagcta atcccctcct tcatcgggac gaatcggcta ccgtcatgga
    11101 ttgcaatatc aagaatccgg tcctggttgc aatcggtcgc ccagtcgtac aggaacgagc
    11161 ctgtttcggt cgccttttcg tcatacttga cgagatggcg ccaatcaaac tgagcataaa
    11221 gctggtcctg ggcattctgc aaaaacatgt ccaggatagg ggcattgacc cccgacgacc
    11281 cggcagcgcc aaaccctagc ctaacggcca ggttgctgcg aagttcgcca agggtcttgt
    11341 aggtcattct gcctcgggct gcgcttcttc agcggctttg cgtccgcgct ttgcggggcg
    11401 gaacgcatgg gcttcgaggc cacgggacga acgcccaaaa actcgctccg cgtagggctg
    11461 gccgttgtcg ttggtgccgt agtaagaaat gagacggtcg aattccatgt ctgcatcgac
    11521 ttcaatcggg tccgccgctt caaggcgatc cacgatgtcc accgatccgt cgccgtgaat
    11581 atccttgagc aattcaactt catgttcaaa gaccgtcgcg ggaagttttt cggccatgtc
    11641 acgggatacg acgaccaaaa catgcttggc aagcatttac aggctcctta aggaattgcc
    11701 ccggcactcg gccggggcgt tttgattagg ctccgatcag aaggccatcc accgaaccac
    11761 tggtaaaggc cgtcacattg agccgcatgc ggcgataagc cgtcaccacc gcagatttgc
    11821 tgcccgaatt ggaccccgga gcggtgaagg tcagcaggtc ggtccaagtc gccgagccat
    11881 cgaccaccgt atccaggtca ttggtgccct ggatcttcgc ggtgcccaca aaagcgccgt
    11941 tcgtcggctg aatcacggca tagacgtacc cgccacgctg gaaggcagcg tccgagtctt
    12001 ccgccttctg cgaagtgccg gcggttgcgg aagtgagggt gccgagggaa acagatttga
    12061 tactcatgtc ggctctcctt taggccattg ccatgacggc gtttgcgtta gaacgattgg
    12121 tggtcagcgc accacgccag gtaagcgccc agtaatagac atatttgtca tacacacggg
    12181 gcggcttgcg agtcaccatg tcctgaccgt ccatcgggcg cagggtaatg gtcctggaat
    12241 tgatgaaata gcagcgcttc gtccagctcg gagtgggcga cacgaatccg ccgaagttgt
    12301 cgtcgaactc ggggcaccat tgaatctcga cgcccttgta atagacgccg gagacgccgc
    12361 catccagctt gcgcggctcg ccgccggcat actggatttg ctggccggag tagccgaggg
    12421 ctgcgatgta gttgtcaatg aaggtcgcgc cggcaaagat cagatcgggg cgaccaccat
    12481 tcttgacaca cgcacgccac atggtttcca tggtgccgag cattgacaac gccgttgcct
    12541 gggtcgtcac agcggtaata ttcagcgtga cgctgtttct ccaccagacg ttcgacgaac
    12601 tggagcggtc gatgccgccc acggtgccgg ttgtcggagt ggtcgaaacc agggcatcaa
    12661 ggccggcgac ggcatcggta gagctggtgc cgtctaactg caagtacatc gagaattttt
    12721 cctcgaaacc gaggcgcaga gtttcgacct gatcttgcag cagagaggtc aattgcaccc
    12781 gttcggcagc cgaggcgttg ccgccgccgc cgctgtcgtc gatggaaatg ccgttctggg
    12841 caagccgatc ttcgtcaagg gaaaagccgt catgagccga acgccagggg aactggctct
    12901 gctcgatgtt gacgcgcttg ttgtaggtca caacggaagc gccgttgaac cattggaaat
    12961 tcgactgata gcggtagcgc agttgctcga tgatgtactg cttaccgcct gggaaggact
    13021 tcttcttcgc catcatcgcc ttgaggaagg ggcgttcgac ggcgacttga tcgatggggt
    13081 tgttcttcag gtagaagtca atggcaactt tgccggcgtc ctgaagttcc tgggtggaaa
    13141 agggcatttg gaaactcctt aagcatgttg ggaagcccga aacgggcgac tccaggggcc
    13201 aaactggatc gagccgaaca tgcagaaggc gaaacctgcg aacgcagcct gaactacttc
    13261 attgcgttat acgcgcaatt tttcatcgtc agaaatacta aatttaactg aatcgtcaaa
    13321 aggaaagggc gctatccaag ccgcccccgc cgatcatccc gccgagtatc ccaatgcccc
    13381 ttccaaggct tcgagcatgc ttgcgggctg cttcgcccca gccgattggc cggatgctct
    13441 gagcggcgcc ggcgtgggcg gggctggcct ggccatgggc atcgcggaga tggtgttgta
    13501 taggatgcgc acctgctgtt gccacatgga cggcggaaag ttgctggcga tttccggcaa
    13561 ctgcttcagg atcaattctt ccttagccgt aaaatcgggg tccgtcttgg cccattgcgc
    13621 cgtcatctga tcgatggccg tcaccgcctg ctgacgctgc gccatctcat tgtgctgtgc
    13681 ctgggtcgct tgctgttgcc gttcatgggc gctggtctgc tgccgttgga attcacgggc
    13741 gcgggcgatc tccatggccg cctgttcgtc catccgataa gcctcgaccg cttggcgcag
    13801 gtcagggtat tgcgccagcg ggtcggcgcc aggaagcggc ttgccaagcg cgaatgcgat
    13861 catttgccgc tgttgatcga gcatcgtcag cgccccctca aggtcgccgc tcttgattcg
    13921 ggtcatgtag tcgatagccc gcgagaactc ctcggccgtg gccccggtcg attggatcat
    13981 ctggcgaaat tcgccgataa cctcgttcgc ctgagcaatc tggtcgtcct tttcgtggag
    14041 ccgtgttacc agattccgga accgatcctg cgccttgctc gacaagccct cgggcatttc
    14101 cgtctcatcg gcgggctttg cctcggcctt ctgaacctca ggcttctgct cctccggctt
    14161 ggtttcgctc gtcggcgttt cttccttctt ggcaaatcgc cccttctcgt cgcgggaaac
    14221 gccgtcatct gttttcccgt caagctctgc attgatggca tcaagcagcg ttgacggctc
    14281 ctgctgctct accggctcga tggcttccgg ttgagtgact tccggcgatt gcaactcgac
    14341 tttttccatt tctcttctcc tagaggatca acaaaatggc cgtttcttcg gcctggatac
    14401 gctttgccct cgccttcgcc cgctcgataa cgtcggccgt tagtgaagcg cctagcaatt
    14461 cgtctctaat ccgccccgcc gtctctggtg gcaacggaga ttcaatctct tcttgcggtt
    14521 tcttccgctt tgcctttaca ggcttcctgg gcttgtccgg agtgccccca agggaggcaa
    14581 atagcggccc ggtaatgaaa gacacatcag ggctcaggtt gatctgcgta cagtccgccc
    14641 cagagaggat atgaacctgg gcaatcgccc ctgttccgga cgtattgctt tgactggaat
    14701 ccgacccggt caggttgtgc gtctgagaaa tcgccccggt gccactgacg ttgtcctgcg
    14761 cgctattggc cccaatcaga tcaatgctcg atccaaggct tatggcgcct gtactgctga
    14821 tattgtcctg ggtgctgttg gcaccgctca ggatatggac ctgggaaatc gatccagtgc
    14881 cgctggtgtt gtcctgggcg ctattcgtcc cggttaggac gtgcgtctgg gtgattgccc
    14941 ccgtgccgct tgtattcgct tgggtactat tcgccccaac aagggtaatt tcacccccgc
    15001 ttgataccgg ctggccgcta accggaaggc cggatatggg ggcgaatggc agcattattc
    15061 aggccagccg atagaccagt cgtaatccaa gacggaatgg gcgtcctctt ttgccgatat
    15121 tgcgtcgcga tgtttcccgg aaacaccggc aatcatcgcc tcaagccctg caagtgcttg
    15181 tgccttgtcc ataaccttct caacgatcac ggacaaatcc acgccgcgag catcggcttc
    15241 ggcttgcaac atcggtccgc ctccggcctt ggcttctgcc agtttgagcg gccatgacgc
    15301 ggcttcgtag ggggaaaccg gcttgatata tcgatccctc aatctggacg cgtgagtctc
    15361 gatcagccag gagacgagat tgacagcatc ggcaattgaa acatccgggt caacacaatt
    15421 gacgtactgg ttcgtttctt cgtcgtagtt gatcatggtc acatccagat gaaaatggcg
    15481 attccgtcac cgcctttgcc gccgttgccg ggggttccgc tcgtacccgt atttccgcct
    15541 ccccctccgc cgcatccaag acctccattc ccaccgtgcc cgcctgtaac cccagcgccg
    15601 ccagtcgtgc caccgccacc cgcgccaccg aatgcgacaa aatgaaatgg cgatgtgttg
    15661 agtaaataat acccgtcggc gccgttccct gcgtttgccg caccgcctgt cgttccgccc
    15721 gcaagggttg gataacgagc cgtacctgta acgttaccgc ccgcgccacc gtttgagcct
    15781 gccccgcctc caccaccgct ccaggtccgc gtaccaccgc tccaggtaat ggcgccccct
    15841 gcaccggcag caccggcagc accggctact ccgctgatgt aagtagacaa cgcaagccca
    15901 tgtccgccct gattggccgc tgcaccagta cttccggcag cgccagcagt tccggcaaca
    15961 gctcctccac cgcctccagt agtccccgtt cccatgattg tgttgtttgt tacgttgcta
    16021 gcgtccgcat agacgaatgt cggctgccca gcggaaccgg ctgtatttga tgtcgtgacg
    16081 cgagcgccac cacgacctac gttcacccac agcacatccg ggagcagaat tgctgggaac
    16141 aacgccagtg ttgcgccgcc tgccccgcct ccaccaccgc ccgaaactgc tgctccagga
    16201 ttaggggacg cgccagaacc acctccggca agtaggaaca tcaggcacat ggaagcacca
    16261 cgcggcttat tcacactttt caattgctgg gaagttccgg cagagtccgc cgtaacaatc
    16321 tgaacttccc cagactcaag cctggggaac ccgaacatat tgattgctgg cattagtatt
    16381 tcccgccgac gcccgtcacc atccatcccg ttccagcggc gccggtagaa gtgccgaacg
    16441 agcagcacac gcgataccca ggagggatgg gaagattgat cgggacttca acatccaatg
    16501 atcccgccgt ttgtgacaat gtcgttgtag ggagggatac atccttgatc aaccacgtat
    16561 tcgccgcagt attgccggta aatgtccccg taatactgga gatgtacaca cggaaagctg
    16621 tcgccgcagg ggaaccaaca gatttagcca tgagtttctg gcaaaacccg ccatttgtgg
    16681 catcggcttg ccatacggaa taaaccgttc cggttccgtc ttgattggtg tttgccgttg
    16741 ggccaatgac agcaccgcca cccgattgaa tgtcaggggt tccgcaaaag attggatctt
    16801 ggttcgctgc catatgtcac catgttggga aaattagacg ggccagttgg cgcccaagtc
    16861 gggcgttctg ggaaaggtcg gcaataagtg tcaaaaacac atccggggca cttgaaaacg
    16921 agacttccgc gttggaattc gatgacgatt caattttgtc tcgggtaatg ccggtcgtcc
    16981 cgttgaacgt tccgtatcca cattcccatt ccgaacctga aacggcacag taagggacga
    17041 tcacagcagc cgatccacaa ccagcggcaa acgtctgata acccgttttc gcgcccgtca
    17101 ggacaaaatc actcgtcccg gtcgcggaat tcgtttccca gacacgatca gcaatagatg
    17161 gcattacgtc ggctggtttg atgtgtaggt cagttgcggg aaattgaccg tgtttcctga
    17221 tgtgatgacc tgatccgatg tttcgtcagt cacccacagc acctttgcac ttccatcagt
    17281 gaaggcgata tgaagattcg gagtcgcgcc agagttggcc gttgcagtag cagatttccc
    17341 cgctgccgac tgcacttgtc gattgttccc ggatgaactg acggtgtagt cggtcccggt
    17401 catcgtgacc tcggcgcagt tgttgcccgt cacggttgcg tagctgtccc cggccgtata
    17461 tgccgagatg accatcatct tggtggcatt gttcttgata tggttgatcc cattatcaag
    17521 tacgtccgca tgtgcccatt tcgccatcgc ttactcctag ttcaaggtat ggggaacagt
    17581 ctctacccca atcatctcgc ccgtctgcgg atcgcggatc acgcgcttag gcgccgtcag
    17641 gtgagcttgt agcgccgcca tctggtcagc aaattgcttg ctcatagcgc acaaggcttc
    17701 gtgcatctgg caaacggctt cgataacctg gggttctgcg ccctgcgcta cgggctgctg
    17761 catcgcggct tgttcctggg ccttccgtgc cttctcgatc tcgacccgct cgcgactggc
    17821 gatctcttct cccttgctgg ctgaccgaat gcgctcaatc tctatctttg ccgctgcatc
    17881 ggcatccgcc ttgatctgcg cttctgcaat cctggcctcg ttgtcagcaa ggtctgcggt
    17941 cttctgatat tcaaatttat cgcgctccaa gcccatggcc tgggatttat cggcaagctg
    18001 cgcctgcaat tgctgattgg ccgcctggag ttgctggagc gcatcctgca tagccggaat
    18061 ttgttgtagg gcctgttgta tttccgctgg aatctgcggc ggctgttgtc catcgggctc
    18121 atgggctttg gcgggcggta tgaaggactc aatatccaac cgctcatcaa atcggcgcag
    18181 cgtctcctcc atcagtttta tgatggtggt cgccatatcg tcttggccgg ccgccctgag
    18241 ttgcgcaacc tgggtcaccg cctccttgat ctgcggcagc atttgcaccc attgttcacg
    18301 ctccttggcg cggtttggct tgcccgtcga tccggcgcga atctcgatct gcaccaagtc
    18361 aaagacttga tccttggtca tctgcggcca caccgcgcct ttgccggcaa tgcgctggac
    18421 gtcttccagg gtcatttcct gcaagcacaa ctccgccgcg tattgcgcca tctcggagat
    18481 ccaatcctcc acgacgtctt gacgctcggc catgcgcgtt tgcaagcctt gcgccatgat
    18541 ctcggcctcg gtcgccgttt tcgccttgtt gatcgtcccc ttcgatgcgt cgccagcgcc
    18601 aagcaccaat tctgcatccc gcagaatcgg ggcaacatca taagtcatgg gatcaacggg
    18661 cggattcggc aacaccgcaa catcattcgt gatcggcttg ttggggtcgc cgccaatgcc
    18721 gatccactca ttcgatgcgc ggttcttgag gttccgaaca tccgcgtccg acaagtcgcc
    18781 ggaatttctg aacactcgaa cagggaggtt ttctttccga tgctcagaaa aattggtccg
    18841 cgtggtgttg tattcgtcct gcagttcgat caggagcgca gcatcggaaa tcggcacaac
    18901 gcttccgtca atcggattga aggcgaggcc aaagaaggga tagaaccttt tgcctgatag
    18961 tgtcggctgg tatggttcgc gagcccattg atccgcccca gcgcacaggg tgtaaatcgt
    19021 attgcttgcg cggtcccagg tttcaaaaac tgccaccaga agcggcgcat cattgttatt
    19081 gctgccttga gtcttcttgt cctgcccgaa ccgcgttgcc gttttgggcg gctccttgcc
    19141 gaattgctgt tcgtattgct ctgtcgtcat ccacacacga tgtgcgatag ccccggcgtt
    19201 ctcgtagccg tcaaactcgt acatggtctc atcgaggatg aaaatatcct cggttaagac
    19261 gtgatcgacc acgatcccgg aaaaccgggt gacttccaca ttggcattca aagcggcgat
    19321 ttgctgctta agctcgcctt ccttggcgtc aagatcggcc ttcgccccgt ccccctctgc
    19381 caattcgtcg cgaaggtatt ggatgcgctg caaattgtcc tggacgtcag caatgcgcga
    19441 ttcaatcaac ggatcgcggc tgatgtcttt ctgccaggtg acctttgccc agccgatttc
    19501 gaccgtcatg gcggcgcgga tcgatgcttt ggcccgcttc ttgagccgtg catctcggat
    19561 gaacttccga ttgagaaccg cctgcatggt cttgcagaac tcgcccaccc atgggtaagc
    19621 ctcgtctgta gccgattcgc ttggcgtgac cgcgatttca ggattcttgg cgtaaatctg
    19681 cgggatgatt gccgcaaaat tggagtgaac aatgttggtg cgaaccagcc cgcccgctcc
    19741 atcgtcgcac acatcgccgc gaacatactt ccgatatttt ttgtattttt cttctttttt
    19801 cgcctcgcgc tgctctttca gagcgcggtc taggcgcttc ccccactgcg ccgcaagggg
    19861 gatggactcg gctttctgct cgtcagtgcg ctccatacgg cttggcctcc gcactgactg
    19921 acaatcccgt tgcctggtct agcttccgca gcgcggcgat ggtatcggca agcccttctt
    19981 gcgccagggt ttccgcctgc cacggccaga tcgcacgaac gacgatgcat tccccttcca
    20041 gggttttccc attgaccgtg taaatccagg catctttgcc agtctctgcc ggaatatgcg
    20101 cttcaaattc gggggatggg tcgatcaaag ccaagagttg ctgatgatcg agcgaggggc
    20161 gagagactga aagcccttgg caaacgcggg aaatctcatt catggcggca atccatgatg
    20221 caattcccag gttataaccc aggtcaaatc atcgtcagaa atactattta cgtcgaattg
    20281 acctgtattt gctgatttcc ttcggagcgt cggtaatcat catcatatgc tccgcagttc
    20341 cgggccgcgg gccgtcttcc ttggggcgtt cgctgcgaat gatgggccgc gccatgacgc
    20401 catagcgcag cgcatccccg gcgtggtctt cctggtcggt gtcgatgtcc tctggattat
    20461 ggtcatcatg ctgcagcgtc ggcagcgtgc ggatgagatg cacgcacgta tcgaagatca
    20521 ccaggagcgg aagcccgtca tcgtcgcaac taagcctgat tcgcatctgt tcccatccgg
    20581 gacggcgctt attgtctgca cgccgccaac gaagaccttc gcgccgcatc atctcggcaa
    20641 ttgatggccc tccgtcctcc gtgaatatcg cagggtccgc cacgccccat ccagtatcgt
    20701 gcgtggtttc ctttccatcc atgcggatga tttcacgagc cacggcgtca gcggtcatct
    20761 tgagtccaac atttggatgc cctgattgca tcccgtaata ctcgcgatag acgatcagcg
    20821 ccccacgggg gaatgccggc attgatccat ccgatactgc aatccaatag caggcgaagg
    20881 gcttcgcaga accccaatcg aatgcgcggt aacgcgtcca atgtatcggg atcgagaatg
    20941 gcttgataac gtgcctggag gagttccact cgctgaaaaa cgcccccgca acgatattcc
    21001 aatccccttc gagccatgcc ttgcgcagca cttcattgcc gccggtcgct gctctgatgc
    21061 gctgctggta gtgtggatcg gcccgcaaca ggatttgatt gtcggacgtc ctgctcggaa
    21121 tgaacatccg aatgaacccc gtatcagggt ctttgaacgg atgatacgga ggaacgtttt
    21181 cgatataccg ctgtttgacc catccatgcc caactccgcc ggggttcccc gttgcccgca
    21241 tggtgcaagg cactccgaac gcactccgca gcgtcgaaag catcttgagc aagccgctag
    21301 gcgtgtggta ctcggtcact tcgtcaaagc tgatcccggt gtattggtgc ccgtggtatc
    21361 ggccataatc cttctccgtc tcgatgtatc gcatcttgac cgtagcgcca ctaggccaat
    21421 accagcaatt cgagaatgga tgctcggccg aagattgcga tttatataca gcaccttccc
    21481 ccggaaaaat ctccgatgct ctggcctgca attcctccaa ctccggatag gttttccgga
    21541 acatgatccc gcgccatttg tcccgataac gcagggcgcc gtcttcctgg tagccgcatt
    21601 gcgcatcgga cttccctccg ccgcgctcgc cgccgtagaa tatttcatcg caccaatcgg
    21661 ccagcagaaa cgatgtctgc gcccctggtt gtgggtccca cattagacga tcccgtgatt
    21721 cttcagccac tcctcgcggc tcatgaccgg acggaccaac acgtcatgct catacttgac
    21781 cgcgccgccc ccttctccgg tcatttcggt ccgcgccagc ttcgggatgt ggtactcaat
    21841 catcttcagc cagagatcaa gcgcccggcc cgggtcgtca ttcgcgacgt cacgcagcca
    21901 ttgctcaatc tctggagccg tcgattctgc gacgcgggca acgacttcgc gaacctcccg
    21961 cgtcaccttg ttgatcgccc ccttctttcg tccttgtccg ctagttctgg tcttcatggc
    22021 gacctccttt caaatcattc cgtgcttcag gcgcaaggcc ggttacgtca gcgttgcgct
    22081 gcgtcgaagc gaatgcgctg ttaagccctt gcgagccgcc caataactca atcgacaatc
    22141 gatccaattt gccatcgcgc ataaactggc actctttgtg ctttcgcttt cgcgttactt
    22201 ctcaaccaaa attgtctgga aacattttcc gctgaaaaac cgccgaagcc tgatgtcgcc
    22261 tcgatctgcc gcccttctga ggacatctct aacggttcca ggttgcgcgc ctgtttccct
    22321 cgcgagttcc gccacagtcc gataaaacgc ggccttttgc agtaccagct tcccgattgt
    22381 cgcggggcgg cctctcttgc tcatgagctt tccgccccct ttgttgtcca gacgactggc
    22441 tgcactcctc gccgctgaac cagccatgac gacgacccgg cgatcttgct cgccagcatc
    22501 catggcagcc gtcgcttatc tccctgctcc atggcctgct tgcgatgagc ggggagaatt
    22561 ccggcgcagt tcttgacctc tatgcaccat tccatccctt gcgggtcttt cgcgatcagg
    22621 tcggcggatt tgatccccgc ggtgagcgtg tcgacagtcc aatcccgatc caatagcatg
    22681 gccttgaact cgctttggcc gcgggcgccc ttgttgcgcg agagttttcc gctcatcctc
    22741 tgaaccccag catcccagac tcgatagccg cgccaatggt cctggagatc atttcccatt
    22801 gcatcggcca catttcagga ttgcggtgca tctcgtcgtg gtggattcga cacagcggca
    22861 tcgttgccca gtccggagct ttcgtccctg ctccgctcat atgcccgacg cccttgatat
    22921 ggtgcgcgtc atctgatggg cagccgcaca ttgcgcacgg cagcgacttg acccaggcca
    22981 tgtaagggac gtttctccat cgaatcgatt tctcgatcac agttgaaccc ctttctcaac
    23041 cgccatggcg ttcagccaat ccagccattc cgaaaattcg ccccgcttga agcggctcgt
    23101 gcgctgacca agcagcacca tcccgccgga aaggcccatc gccacgcgag gaacttcgtt
    23161 gcgccaggca caggtcaaaa tgtccttcca atcctctgcc gtgatcgtct gcatggcgcc
    23221 attgacgggc cactgcagtt gctccgcgaa tgcatcgagg atcggccatt gcgccgcgtt
    23281 ttggtcgagc gagcgggtgc gctcggaaat cttgaccgcg tagccattcg gggctgccat
    23341 gacataccgg caagcagcct gtcgggccac atcagaattc aggatcagga ctttactcat
    23401 cgtccgcccc tggttcgcgc atgtaatgct cgcaggtcgc attcctgggg acgaatcgca
    23461 cccatgcgcc gcaccacata tgctcaggtg actgtactga ccaggccgca tggacgcacc
    23521 agcggcagaa attggcctcg gccttgaggt tgattttcag agggatcgtc atcgccaccc
    23581 gcctgacacg cagattgccg agcagatgat cccgacggcg taagctatcc aaaatttccc
    23641 cgctggcccg agcgggattg ttcccacagt gtcatcgcat cggcgcggag tcggtcggcc
    23701 gcttcctggc cccgatgctt cgctaccagg gtcatgtagt ccagccgctt tggttttaaa
    23761 atcgtgagca accaacgcgc ttcgcaggct gcgcgatggc tttcgctcca ggtacatgtc
    23821 ggggattcgc atggattcgc tccgcacaat tcaaggtcca tcaaagcaca ggagatcgac
    23881 caaaattccc tgatcgttct cgacgaactg ccagctctca cggtcaagcc acaagccgat
    23941 cttcccttcc cactccccgt tacgctgttt ggcaatcgcc aacatcgcgt cagcctgcat
    24001 cgagagcgaa actttctcgt cggaactcac aggcttaccg ccaagacttc cgttcggatc
    24061 gaaagcgcgt tccttgcgaa cgttgcgcca aacgctgatg acgttgtccg cctgatcggt
    24121 gatcgcagat gagccgcgca cgttgaattt accgggggtt tttccctcgt cctcatcggt
    24181 ggcccccttc ctggcatggg caaccagatg gatatgaatc ccggtatcgc gagcaatgac
    24241 gcacaagtca ccgatgaact gcttctgcgc cgtcaatgct ccgttcccgt cctctggaag
    24301 cccgcaggtc agcaaagagt cgatgacaaa atgctggatg cccttcttgt cactgcaata
    24361 gcggatcacc gcaagcaatt tctcgggcgt cgtctggccc aagtggtcgt aaagccaaag
    24421 atgctctttg acgttcgcca agtacccgcg ggcgaattcc gcgtccagcg tatcgtgccc
    24481 agcagcttgg cgaatcaagc gataaagcgt ccgcctcgcc ggcatctcga aactcgcaat
    24541 gcagaccttt gagcgctggc tgaccaacga cgcgcagacc atccccagcg cgtagctttt
    24601 tccgtggccg ttgtagcccg tccagaccgt gacctcggcc tggcggaacc ggaatcgcg
//
LOCUS       DAOQEW010000034        24630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_482_length_24630_cov_9.078087, whole genome shotgun sequence.
ACCESSION   DAOQEW010000034 DAOQEW010000000
VERSION     DAOQEW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24630
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_482_length_24630_cov_9.078087"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLS35_05735"
     CDS             complement(<1..851)
                     /locus_tag="PLS35_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18191.1"
                     /translation="MAVSQMFPGNHPSSTSSSDPIDSVRETSLAFQRVALLYQAMPAA
                     LIAGLGCAVALVFVNWNVLPHDRLLAWLAYHLLLSIGRYGLARSFAATSPTPSTIRRW
                     DRAFLTGTLLAGLGWGATALLVFPESSLAHQVFLAFVLGGVSAGASSTLAARFDAFLS
                     FAVPTLAPLILRFFFLNHEQALPMAICTLLFSLLMVYTAYRTSNNVREMLQLKYHNAQ
                     LVDQLSSQLEEGEQTALLLNVHSEHYRFIMEYAQDIIYRTDRSGRFTFINPAVIRLLG
                     YHETEMLG"
     gene            complement(958..1587)
                     /locus_tag="PLS35_05740"
     CDS             complement(958..1587)
                     /locus_tag="PLS35_05740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI18192.1"
                     /translation="MSIYATYIFPRLMDWVLRGERFQTERRLVLAPAHGTVLEIGFGT
                     GLNLPHYPSTITDLHTVEPATLLPDRVARRVANAPFPVHLRQLSAEVLPYASGLFDCA
                     VSTFTLCTIPDPCAALREVRRVLKPGGCFLFLEHGRSDDPAIARWQDRLNPLQQVVAC
                     GCNLNRRIDRLITQAGFRVEQLDRFTLPGVPRIGGEMYRGRAGTAGRST"
     gene            1803..4238
                     /gene="leuS"
                     /locus_tag="PLS35_05745"
     CDS             1803..4238
                     /gene="leuS"
                     /locus_tag="PLS35_05745"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNI18193.1"
                     /translation="MSKSYDHQAVESKWQAYWETHRPFRASDDPSKPKFYCLDMFPYP
                     SGSGLHVGHLEGYTATDIVSRYKRMRGFNVLHPMGWDAFGLPAEQYAVKTGVHPAITT
                     AQNIATFKRQMKRVGLSYDWERELSTTDQDYYRWTQWIFLKLFERGLAYVAEVPVNWC
                     PALSTVLANEEIVDGKSEVGGFDVIRKPMRQWVLKITAYAERLLEDLSLVEWPTSTLE
                     MQKNWIGRSIGAEVDFALADTTGNLRVFTTRPDTLFGATYMVVAPEHPLVDVVTTPSQ
                     RAQVADYRDAAARKSDLQRQELDKVKTGVFTGGYAINPVNHERLPIWIADYVLMSYGT
                     GAIMAVPAHDERDWAFATQYHLPIREVIQGGQVDQAAFVETERGRVINSTTPDGSCSL
                     DGLLPVDAIPKMTTWLETHGKGKKTINYKLRDWLFARQRYWGEPFPIVWVDGEPRPLP
                     EEQLPLPLPETKNFKPSGSGESPLANLEEWLTTTDPVSGQAARRETNTMPQWAGSCWY
                     YLRFIDPKNTRQLVDPAKEQYWMPVDLYIGGSEHAVLHLLYSRFWHKVLFDAGVVSTP
                     EPFKKLVHQGIVLGEDNQKMSKSRGNVVNPDEMIDQFGADAVRLYEMFMGPLESMKPW
                     STRGVEGITRFLDRVWRLMIGEDGALSAAVTNITPTAEQLRLLHYTIKKVTEDIESLR
                     FNTAISQMMVFTNDMTKLDQRPRALLEPFVLLLLPFAPHLSEELWERLGHQPSAGQQA
                     WPPFDPALVVSDRLTIPIQVNGKLRGKLEVDAGASRDQLEPLAKKEVAEWLQGKEPKK
                     VIYVEKKLINFVV"
     gene            4243..4878
                     /gene="lptE"
                     /locus_tag="PLS35_05750"
     CDS             4243..4878
                     /gene="lptE"
                     /locus_tag="PLS35_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNI18194.1"
                     /translation="MPRYLLLLVLGLCAVNITACGYQFRVQGAGPTIGGASEPAAKRS
                     QAPKLAILPISNSTYEPNFEIKLANYLRREFSAGAGAEIVGPSAGADLYLSGQIMQIL
                     LPTLSFDQTTTFESRVEMLVSVRIEDAKTKRLVWSQVAKGTAEFFLTQDLQFNRVLQN
                     RALEQAGRFVAEDLASRFLLFLDTGELDKMLKRPVKAEAVPGGNSPPEAAR"
     gene            4898..6010
                     /gene="holA"
                     /locus_tag="PLS35_05755"
     CDS             4898..6010
                     /gene="holA"
                     /locus_tag="PLS35_05755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNI18195.1"
                     /translation="MSTSVMSMELPQSLARSGVMPLYAVVGEEDYLRDSSVAALRTAA
                     LGAGAESGFNYDIFHGDDSSIADVLACAAEIPVFAERRVVVYKSVEKLPAREGEKLLS
                     YLASPNETTTFIVVGAKLDGRLKWTQALTKRAVTVNCAPLRDAQLSAWIRQEAAALGV
                     RMQEDAIQLLKEVGGESLYAVKRELEKLAAYVPSDRTVQSSDVEALRGTEPGASVFDL
                     MNAIGAHDHGRAFRILARNVENGEAPLRILGALVWQYRRLWKLKEQLKAGGREGEAAR
                     TFRMDPSRVRGFLAQFPDDQMTQAFEWFLETDSKLKGGSGSTPVRVMEDLLFRLCGKP
                     SKPASAAQRVQPAVPPASRPSGSRPVSNVRTVTRKR"
     gene            complement(5994..6269)
                     /gene="rpsT"
                     /locus_tag="PLS35_05760"
     CDS             complement(5994..6269)
                     /gene="rpsT"
                     /locus_tag="PLS35_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNI18196.1"
                     /translation="MPVVHKSTIRRARQSEKRRLRNRATISSVRSILRKVQDAIAAKK
                     PDEAKATLRAATAALSKAVTKGVLKPNTASRRVSRLAVRVNAITASA"
     gene            6381..7097
                     /gene="pyrF"
                     /locus_tag="PLS35_05765"
     CDS             6381..7097
                     /gene="pyrF"
                     /locus_tag="PLS35_05765"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNI18197.1"
                     /translation="MMTRIDARDRLIVALDVPSAAEAEALVDRMGDQVRFVKVGLELY
                     TVAGPEIVRRLVDRGKRVFLDLKFLDIEETVRRATARVAAMGATFLTIHANRKALIAA
                     VQGRGQSDLKLLAVTVLTNFDGNDLRDMGIQRSVRDLVTARAMLAAETGCDGVVASGE
                     EPEAIRAKVGPNLLIVTPGVRPAGNATDDHARPTTPTQTIAAGADYLVIGRPIRDAQD
                     PPAATAAILKEMQAAFDHRG"
     gene            7223..7299
                     /locus_tag="PLS35_05770"
     tRNA            7223..7299
                     /locus_tag="PLS35_05770"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7257..7259,aa:His,seq:gtg)
     gene            7407..7706
                     /locus_tag="PLS35_05775"
     CDS             7407..7706
                     /locus_tag="PLS35_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18198.1"
                     /translation="MRSFRRFPVQCSVYYSCDAFQGSGIAWNLSLNGWRVDGTHPVDP
                     GTVVTLCIFLPGQHPSVFVDRAIVRWSRGQEFGVEVNTIKADEQARLEQFVTALV"
     gene            7862..8206
                     /locus_tag="PLS35_05780"
     CDS             7862..8206
                     /locus_tag="PLS35_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18199.1"
                     /translation="MAKSPTTRSHSRVPVSCFLYYLGEGLVGTGKVCDLSVRGWRIEG
                     DKPVTVGMKLTLRVFLPDEPKAIDVEGVTVKWVKDRAFGLETLKLSAAAEARIHKFVE
                     SVLKSADSSRVA"
     gene            8394..8762
                     /locus_tag="PLS35_05785"
     CDS             8394..8762
                     /locus_tag="PLS35_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18200.1"
                     /translation="MMEQQNRPRFIVECTGSRSEDGLLVWNGRMLDLSIPGWSRTGLK
                     GLQAGDLLQLHLHIPGEPKPLSVRLATIRWASESRLGVDPILMDADDQLRLSDFVSAH
                     TTVPSFSTDRKEQIVISDAG"
     gene            9068..10369
                     /locus_tag="PLS35_05790"
     CDS             9068..10369
                     /locus_tag="PLS35_05790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18201.1"
                     /translation="MTPGPQLPDATHTTPLTPETEAQVDQFAHTDILVGIPSFNNVET
                     IGHVVKAVSAGLAKYFPEARAVLVNSDGGSTDGTQQVVSQAVVDLKTLFIGDQQSSLH
                     KIITPYHGIPGKGSAFRTIFEIARRLKAKACAVVDSDLRSITPEWIELLVSPVFEQGF
                     DYVAPYYLRHKYDGTITNSIVYPLTRTLYGHRIRQPIGGDFGFSGQLAQHYLDKHVWE
                     SEVAKFGIDIWMTTEAIASGARVCQSFLGAKIHNPKDPAADLSAMLVQVLGAVFALME
                     DHYAVWGKIDGSNAVPLFGFQYEVGVEPVNVNVDRMISTFRQGLTDLGTIWDQILAPG
                     TRQSLRPLGTCSLQDFRIADSLWARVVYDAAVAYRNRVLPLDHVLKAFTPLYLGRTAS
                     FVLDTQGLTSSEAEGRIEALCQAFEKDKSYLVARWNEPHAG"
     gene            10427..11134
                     /locus_tag="PLS35_05795"
     CDS             10427..11134
                     /locus_tag="PLS35_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18202.1"
                     /translation="MKEFFEKGLLDPLELMARQVLAVLPSLLAMSIIFFAGLVVAWTA
                     SQALERLLRVVGLDRLFDRLGVTGALLRGGVKTDPSRLVGQAAYWLVMIFATVAALGA
                     LNLQPINDFAKSFLAYVPHLVTAGLILVAGWLLSNFVSQAVLIAAVNAGLPPARLVAT
                     CSRWGIQLLAVAMALEQLGIAQNIVVVGFGITLGGIVMAGAIAFGLGAKDLAKDYLER
                     GLSVRTRDGAPDDLRHL"
     gene            11176..11397
                     /locus_tag="PLS35_05800"
     CDS             11176..11397
                     /locus_tag="PLS35_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18203.1"
                     /translation="MTNKISDIDGEAKARRWLGGEAAVPRYPHRRPSRFVFNRSLLGL
                     VACAISAGFAGGLVVGLLSRRTNTSQRGH"
     gene            complement(11454..12278)
                     /locus_tag="PLS35_05805"
     CDS             complement(11454..12278)
                     /locus_tag="PLS35_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNI18204.1"
                     /translation="MQRILIFSDLDGSLLDPTTYSYKDASDALAAIEHRGATLILVSS
                     KTRAEMEPLRRSLCNHHPFIVENGGAIYVPNGTFPFPLEGALSREAFQVFQLGAPYAT
                     LRAALKEIGQEIGGRLRGFGDLTLAEVVQLTGMSAGDAQLAMQREYDEPFVMDGDVVA
                     WQDLLAAATRRGLCCTRGGRFYHLMGANDKGMASRQLIEWYRRLEQQKGQTLVTVGLG
                     DSLNDLPMLESVDYPILIQKPNGSYDQDVQLPRLIRADGVGPVGWNRSLIDLLPTL"
     gene            12519..17276
                     /locus_tag="PLS35_05810"
     CDS             12519..17276
                     /locus_tag="PLS35_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18205.1"
                     /translation="MRSDKKPPLKSDKKVLPKVQKKGSLGYNKKSALLEDAITQMNAG
                     KYGRASSALKDLLALDPLNAEARRLFATLHLRLGSLMSARTAFESLAREAMERQDYWL
                     AESLLREYLTAGPRYVPFLEMLGRVYEEKGDVMAAVAEYGKAVEVLLEDPDTEKPNHP
                     SELFARIRSIAPGSPVSFRLAAMFDTVTGQVLQATPQPAVPTQEHEAPQADGVEAPAS
                     AVAPMPWEQIEPLQADVPVAQAPSSPSDVPPTAEVIEPALSAALPVEAVAAVESQKTE
                     IDQSPTLESSEVRTTLTSADSVAPAVPAVGSSDEPMKAAAESEPPAIDPLPALSGEVE
                     SAPPTIPVELPPTNVALVEVGADPIAHAPSSPAPMPWDQVEEVAVVIPPASPSQESTV
                     SDEVAPIALESEVGPAVTVEAVLPSMELPAETGSQTTPSTPAPLSGNQVVEEIIAIPP
                     ATSEVSAVPESAQMPTSETEWSSAPPSDPAPRAIEVLSQSGSHPIVSTPTTPVSMPWD
                     QVEENPILIPPAAGSSAPSVEEVAPATCEETPLPPVIEAGKSDTEPPAQQAEAQTAAP
                     SASSSSEITSSGLSWDEILAAVAAMQSSPASPQSLPSAEPTPATESTVMEEPATAVTA
                     PSDAVEMTAVAPDAPSSEPSLDLGSASSTLSAPMPWEQVEVEDVAIPRQEPEPEFGSA
                     TIAVEETGNSSGMALDSTLVVPAVPEPVPQVEPPTVAAESSTGSVEESGRGFRLLSSD
                     GQEPHDTPLHQPADLPEVTLAEKSPTPSDPEPTVELVKEESAAIPLRLVERESLPSQA
                     AVEPAPVKAEPITEFTLAGTPAESIETVLEAAPQVEDSLELVGAPEAMMPSGSAPVDE
                     IVEPIPLSSVEEQTVSLAEPIALEAQVEPPAEPSSEESEPAPVLALEPAAKPDEHVVV
                     DSVVTAAEVSLTELTEVPTASAEDAPLVAPHELSTVVPAEESSELVQIPQALEAVQTD
                     QPVDMAASPVPALEPEVPAPAVSAPAEVPASDGLHILWEDASSAPTPKPSTGNMLTRW
                     LKKPAEPVAPEVSAVPPATPVPAEPVVPVASQAVEESVPVAVVMEQPYEHIEQAPAAV
                     VEEPTHRPTRIQSAGGGVWSRVAEAAASLVGAGVSTTRSLVVLVLALVGLVLFVVGGT
                     IAAVALTWLILEEQPNAAYRTMTSVPQQTLQDSSKNGYLLLLGLGAAASQDPVQVGLD
                     RRIEGADRAFTHTCLTGEGYSPDERQSASADAAGKWMKAADPALQMQMDVSGAKTWLS
                     QAEVGLSRYRQWLNKPFEDVGYGQSISPNCGLILQTHRLYLADGFAQDVDTGFARLET
                     DLTAWRTVLGQAKSLPVKMLASAAMNDDAMIMDGLLQRPDLDERYVSRMAKLAKPLEP
                     SEQSLRWPMQSQFVLATKTLDEAVSQNRAETRPFYGAIAAALPLPKQRRFNAYAEYYD
                     AAGKAAADGRYTDLPKLSQFVRVAPYSVSDMVMNPIEGLVGVDSMPTWETYAGRVLET
                     DARLRLVSLQAWLRRTPPEQDLLTRLAKAGQGLYDPFTGLPMLVNMKKGVLYSVGPDL
                     KDNEAHERLDLVAQIPSVAWAGGKKLADTGKAK"
     gene            17623..18240
                     /locus_tag="PLS35_05815"
     CDS             17623..18240
                     /locus_tag="PLS35_05815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNI18206.1"
                     /translation="MSGCHSPIAVPGQPGHLCLGERVASLDQTQALDRFLAGIERRAF
                     RMAHIATGNADEALDLVQDAMLKLAEKYARRPEEEWGALFHCILQSRIRDWYRRERVR
                     NRLREFFRGAQDDEDAEDPLEQIPDSSTPAPDGEVQRKRACAELEVALRALPLRQQQA
                     FLLRIWEELDVAQTARAMGCSEGSVKTHLFRALQVLRQRLGEHWP"
     gene            18237..18593
                     /locus_tag="PLS35_05820"
     CDS             18237..18593
                     /locus_tag="PLS35_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18207.1"
                     /translation="MNERSDEQPDPLSAAAKRLLDHRLQELDQKTVLALQRARVNAVS
                     RSSARRPWLQWAGGVALASMAALAVTMWLWQANGANHSHLLLEDLELMQSPENLELSE
                     DLEFYDWLADGTATTG"
     gene            18894..19400
                     /locus_tag="PLS35_05825"
     CDS             18894..19400
                     /locus_tag="PLS35_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNI18208.1"
                     /translation="MNVLLVGIMAVFIALSPAWAQGDQTGVPWSQLSPGEQQLLQRFS
                     DNWDQLPPRKQQRLRNGARQWGTMTPEERQEAKQRFKEWRSLPPEQRQQLRERYQQFR
                     QLPPERRESVRDARRWFRSLPPEQRKELREKFQTMSPEERRAYRRELRRQYGGSGGGQ
                     APAGVPGQ"
     gene            complement(19431..20396)
                     /locus_tag="PLS35_05830"
     CDS             complement(19431..20396)
                     /locus_tag="PLS35_05830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNI18209.1"
                     /translation="MSGSPSNRRILLLHHDPVEIERLTAGLSRAGFSVVVADAGRLAL
                     HELVHDPPCLVLAAEGTNGRSADTLAKELRADPFLGRLPVIILIRDSRVNDVDWAALG
                     VDDYIAVPYRPEEVPQRVRLCLSRLERSLDANPLTRLPGNSTILHETTARIESGAPFA
                     LAYLDLDNFKSFNDRYGYARGDEVLVVTCRILTTVVSELAGTDGFVGHVGGDDFVFMS
                     APATIEAICQTLIKRFDLVIPDFYDPDDRGKGFIDSVDRRGNHERFSLMSLSIAVVTN
                     EHRAITHPGDVSKIASELKKIAKSQPGSVYVKDHRKAETTDARTT"
     gene            complement(20393..21229)
                     /locus_tag="PLS35_05835"
     CDS             complement(20393..21229)
                     /locus_tag="PLS35_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNI18210.1"
                     /translation="MKPSANVDLRRRIEQVGELPTLPHVVQKLASMIGRPNVSAEEIG
                     ALIEKDQVLSAKVLRLANSPFYGFPSRIASVAHAVVVLGLSVVKGLTLCATAFDMMKN
                     AGMNDLWRHSLGVAITAHILGAKAGMKNPEEVFVGGLLHDIGKVVLYVKWPDVGQQIT
                     AARKDGSRSLMETEQELFAVTHADVGGWLATAWHLPTTLREPILHHHMPTAAQDAKLQ
                     TAIVHVADVLVKGLACGNPGDDLIPPLSRQAWDLVGLDAQSLAQSLAQAAEEFQTIDD
                     YL"
     gene            complement(21226..21561)
                     /locus_tag="PLS35_05840"
     CDS             complement(21226..21561)
                     /locus_tag="PLS35_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18211.1"
                     /translation="MKRVVIEELTPGMILAKPVTNNTGLVVLPPGAELDEATLSRLQR
                     LGLTSVYVEGDAGDASGKTLAELEAELDHRFRLVSDDPIQCQIREAIRQQLRTTHGVA
                     DHQEAPPAT"
     gene            21801..22484
                     /locus_tag="PLS35_05845"
     CDS             21801..22484
                     /locus_tag="PLS35_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNI18212.1"
                     /translation="MKCEILYPGAFPMVRVYLDASETVKAESGAMVAASPTIDIESKM
                     EGGFLGALSRKMLSGEKFFFQTLRASRGAGEVLLAPTVPGEIVLLELDGVNEYMVQKD
                     GFLAGAEGVKIESKMQSLTRGLLGGEGFFILKIGGTGQLVLNSFGAIHKIELKPNEEY
                     IVDNSHLVAWSSTTTYNIEKASSGWIASLTSGEGLVCRFRGPGVVYIQSRNPGSFGNW
                     IRQFIPVSE"
     assembly_gap    22491..22590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22671..23804
                     /locus_tag="PLS35_05850"
     CDS             22671..23804
                     /locus_tag="PLS35_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNI18213.1"
                     /translation="MNATPNALCFGEGLPGAGVPCTVMLVEEGLRVSPMGESALAEQV
                     VTFADMAVEAGGLDHDQLVVSWGTGPSVWTLYVKDPALIVAFRRAAPPQLTAHLERAA
                     EQVRRARHSHRLLWGSVLGVVFGLGLLLWFGTDLMVEWAVARIPIEWEQKLGETVYQD
                     FLSKETVLKEGPAVSAVQEMTQRLTEKIPNNPYKFQISVVQSPVVNAFALPGGYVVVF
                     TGLMTKAESGEEVAGVLSHELNHVLQRHGMERMVKMLGLSAVVGILLGDQQGLTGLAK
                     QVGMELVTLKFGRAQETEADVTGIRLLSDARIAPDGMIRFFERLSEKDKERVEVFSTH
                     PMSAARAALLKAELAALPKRTPEPFTFQWKTIQDSLAVSEPKK"
     gene            23818..24303
                     /locus_tag="PLS35_05855"
     CDS             23818..24303
                     /locus_tag="PLS35_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNI18214.1"
                     /translation="MRIGVISDTHGLFDPSITRHFAGVEHILHAGDIGPRDVLAQLER
                     IAPVTAVSGNVDGFDASGIPVEQTIELAGHRIAVYHRLYEGGRLTGDGIDLLERIRPT
                     VCVYGHTHQPKAEWRDGVLLFNPGSAGPKRFRLPRALGVLLFQNGSIETRHIMLADRA
                     E"
     gene            24426..>24630
                     /locus_tag="PLS35_05860"
     CDS             24426..>24630
                     /locus_tag="PLS35_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNI18215.1"
                     /translation="MLRQIIVALGVVAVASQAGLALAANPDTGPGCGLGKLAWADYKN
                     QKNIAPQVMMATTNGTFGSGTFGI"
BASE COUNT         4890 a         6775 c         7749 g         5116 t
ORIGIN      
        1 ccgagcattt cggtctcgtg atagcccagc aggcggataa ccgcgggatt gatgaacgta
       61 aaccggccgc tccggtcggt tcgataaatg atgtcctgcg catattccat gatgaagcgg
      121 tagtgctcgc tgtgcacatt caagagcagc gcagtctgct ctccctcttc caattgcgac
      181 gagagctgat ccactaattg tgcgttgtga tatttgagct ggagcatctc ccgaacgttg
      241 ttggaggttc ggtatgccgt ataaaccatc agcagggaaa acagcagcgt gcaaatagcc
      301 atgggcaggg cttgctcatg gttgaggaaa aagaaccgca gaatcagcgg ggccagggtc
      361 ggaacggcaa aggacagaaa cgcatcgaat cgcgccgcca gcgtcgagga cgcgcccgca
      421 gacacgcctc ccaacacaaa ggccaggaag acctgatgag ccagtgacga ttcgggaaag
      481 acgagaaggg ccgtagcgcc ccaaccgagt ccggccagca acgttcccgt caggaaggcc
      541 cggtcccacc gtcgtatcgt cgagggcgtc ggcgatgtag cggcgaacga acgagccagt
      601 ccatatcgac cgatcgataa gagcagatga tacgccagcc aggcgagcaa ccgatcatgc
      661 ggcaggacgt tccaattgac gaagaccaac gcgacggcgc atccgaggcc ggcaatgagc
      721 gccgcgggca tggcctgata gagcagggcg acgcgctgaa atgccaggct ggtctctctg
      781 actgaatcga tcgggtcgga cgatgaggtt gaggagggat ggttgcccgg aaacatctgg
      841 gagacggcca tcgggatcac tcccgtttac gagaagcata catgcaaaac actcctgtat
      901 aggcgcagtc tacaaaattg gcgggaggga tacaatttcc ctgtaacata tgaattacta
      961 cgtggagcga cccgcggtgc cagccctgcc tcgatacatc tctccgccga tgcgagggac
     1021 gccgggcaag gtaaaacgat cgagctgttc cacgcgaaaa ccggcttgcg tgatgaggcg
     1081 atcgatgcgt cgattgagat tgcagccgca ggccaccacc tgttgcagcg ggttgagtcg
     1141 gtcctgccag cgggcgatcg cagggtcatc gcttcggccg tgttcaagga acaggaagca
     1201 gccgccaggt ttcaggactc gccggacttc tcgcaaggcg gcgcagggat ctggaatcgt
     1261 acataaggtg aaggtgctga cggcgcaatc gaacagaccc gaggcatagg gcaacacttc
     1321 tgcgctcagc tggcgaaggt ggacgggaaa gggcgcgttc gccacccgcc gggcgacgcg
     1381 gtcaggcaaa agcgtcgcag gctccaccgt atgcaagtcc gtgatggtcg acgggtagtg
     1441 cggcaggttc agccctgtac caaagccaat ctccagcact gttccatggg ctggagcaag
     1501 gacgaggcgg cgttcggtct ggaagcgctc tccgcggagc acccaatcca tgagccgggg
     1561 aaaaatgtag gtggcgtaga tactcaaggg cattcctcgc cgggaagatg gacgcggtta
     1621 gtgccgctct tgctgccgtc tcgccgtgta tttgagcagg agtggcgctg cggatcaagc
     1681 gaagcggacg tgcccattca gaatccctcc cgcggcgtcc ttgttgccgc gtaccgcgta
     1741 tgctacagtc gtccgttctt cagtcgaacg ccaatcacta cgtcacctat gatgttcata
     1801 cgatgagcaa atcctacgat caccaagctg ttgaatcgaa gtggcaggcc tactgggaga
     1861 cccaccgccc gtttcgggcc tcggatgacc cttcgaagcc taagttctat tgtctggaca
     1921 tgtttcccta tccatcaggg tccgggctgc acgtcgggca cctggaaggg tataccgcca
     1981 ccgatattgt gtcccgctat aaacggatgc gcggcttcaa cgtgttgcat cccatggggt
     2041 gggatgcgtt cggcttgccg gccgaacaat atgccgtgaa aaccggtgtc catcccgcca
     2101 tcaccaccgc tcaaaatatt gcgacgttta agcggcagat gaaacgcgta ggactctcct
     2161 acgattggga acgcgaactc agcacgaccg atcaggacta ctatcgttgg acgcagtgga
     2221 tcttcctgaa actgttcgag cgtggcctgg cctatgtggc ggaggttccg gtcaattggt
     2281 gtccggcgtt gtcgacggtg ttggcgaacg aagaaatcgt cgatggcaaa agcgaggtcg
     2341 gcggattcga tgtcatccgc aagccgatgc ggcaatgggt gttgaagatc accgcctatg
     2401 cggagcggct gctcgaggat ttgagcctgg tcgaatggcc gaccagcacg ctcgagatgc
     2461 agaagaactg gatcggccgc tcgatcggcg ccgaagttga ttttgccctg gccgacacga
     2521 caggaaatct gcgtgtcttc acgacccggc ctgatacgct gttcggtgcg acctacatgg
     2581 tggtggcgcc tgaacatccc cttgtcgatg tggtgacgac tccgtcgcag cgggcacagg
     2641 tggctgacta tcgcgacgcg gccgcccgca aaagcgatct gcaacgtcag gaattggata
     2701 aagtcaaaac cggcgtcttc acgggtggct atgccatcaa tcccgtcaat catgaacgcc
     2761 tgccgatctg gatcgccgac tatgtgctga tgagttatgg caccggcgcg atcatggcgg
     2821 tgccggctca tgatgaacgc gactgggcct ttgccactca atatcatctg ccgattcgcg
     2881 aggtgattca gggcggccag gtggaccagg cggcattcgt tgagacggag cgaggccgtg
     2941 tgatcaactc gaccacgccg gacgggtcct gttcgctcga cgggttattg cctgtggacg
     3001 ccattcccaa aatgacgacg tggctcgaaa cccacggcaa gggcaagaaa accatcaatt
     3061 acaaacttcg ggattggctc ttcgcccgcc agcgttattg gggagaaccg tttcctatcg
     3121 tctgggttga cggcgagccc cggccattgc cggaggagca gttgccgctg cccttgcctg
     3181 agacaaagaa ttttaagccg tccgggagcg gcgaaagccc gttggccaat ctcgaggagt
     3241 ggttgacgac gacggatcct gtctccggcc aagcggcgcg gcgcgagacg aatacgatgc
     3301 cgcaatgggc gggttcctgc tggtattacc tgcggttcat cgatccgaaa aacacccggc
     3361 aactggtcga tccggcgaaa gagcaatatt ggatgccggt ggacctctat atcggcggca
     3421 gtgaacatgc cgtgctgcat ttgctgtaca gtcggttctg gcacaaagtg ttgttcgatg
     3481 ccggtgtggt gagcacgccg gagccgttca aaaagttggt gcatcaaggc atcgtactcg
     3541 gcgaagacaa tcagaagatg tcgaagtcgc gcggcaatgt ggtgaatcct gatgagatga
     3601 tcgaccagtt cggagccgat gccgtccgct tgtacgaaat gttcatggga cccctcgagt
     3661 cgatgaagcc ctggagcaca cgcggggtgg aaggcatcac ccgctttctt gaccgggtct
     3721 ggcggctcat gatcggcgaa gacggcgcgt tgagcgctgc ggtgacgaat atcacaccga
     3781 ccgctgagca gctgcgactg cttcactaca cgattaaaaa agtcaccgaa gatattgagt
     3841 ccttgcgatt caatactgcc atttcacaga tgatggtgtt tacgaacgac atgacgaaat
     3901 tggatcagcg gccccgcgca ttgttggaac cgttcgtgtt gttgctgttg ccctttgcgc
     3961 cgcatctcag cgaggaactc tgggaacggc tcggtcatca accgagtgcc gggcaacaag
     4021 cctggcctcc gttcgatccg gccttggtgg tcagcgatcg actcacgatt ccaattcagg
     4081 tcaacggcaa gctgcgtgga aagctcgagg tggatgccgg ggcatcacgc gaccagctgg
     4141 agccactcgc caaaaaagaa gtcgccgaat ggctgcaggg gaaagagccg aaaaaggtga
     4201 tttacgtcga gaagaagttg atcaatttcg tggtgtgatg ccatgcctcg ctacctgttg
     4261 ttgcttgtct tgggtctgtg cgcggtgaac atcaccgcct gcggctacca gttccgggta
     4321 cagggcgccg gtcctacgat cggtggcgcg tcggaaccag cagcaaagcg gtctcaggca
     4381 ccgaaactgg cgattcttcc catctccaat agcacctacg agccgaactt cgagattaag
     4441 ctggccaatt atttacgccg ggagttttct gcaggggcgg gggcggaaat tgtcggcccc
     4501 tccgccgggg cagacttgta tctgtccggc cagatcatgc agattttact gccgaccttg
     4561 agtttcgatc agaccaccac ctttgaaagc cgcgtcgaaa tgctggtgag tgtgcggatc
     4621 gaggatgcca aaaccaagcg actggtgtgg tcgcaggttg cgaaggggac cgcggagttt
     4681 tttctcacgc aagatctgca attcaaccgc gtgctgcaga atcgggcgtt ggaacaggcg
     4741 ggtcgattcg tggcagaaga tcttgcgtct cgctttttgc tgttcctgga tactggagag
     4801 ttggacaaaa tgttgaagcg tcccgtcaaa gccgaggcgg ttcccggtgg aaattcacca
     4861 cctgaggctg cccgttgatg ttggaaggtg tctgtaggtg agtacctccg tcatgagtat
     4921 ggagctgccc cagtccctcg cgcgtagcgg ggtaatgccg ctctatgccg tcgtcggtga
     4981 ggaggattat cttcgcgact cctccgtcgc cgcgcttcgc acggcagccc tgggcgccgg
     5041 cgctgagagt ggattcaatt acgatatttt tcacggcgac gactcctcca tcgccgatgt
     5101 cttagcatgc gcggcggaga ttccggtatt cgcggagcgc cgcgtcgtgg tctataagtc
     5161 cgtggagaag ctgccggctc gggagggcga aaagctgctg tcgtatctgg cttcgccgaa
     5221 tgagactaca acgttcattg tggtgggggc caagttggac ggtcggctca aatggacgca
     5281 ggccttaacg aagcgggcgg tgacggtcaa ttgcgctccc ctgcgggatg ctcagctctc
     5341 ggcctggatt cgacaggaag cggccgcgtt gggtgtgcgc atgcaggagg atgcgatcca
     5401 gttgctcaag gaggtcggag gtgagtccct ctatgcagtg aagcgagagt tggagaagct
     5461 tgccgcctat gtcccgtcag accggaccgt acaatcgtcc gatgtggagg cgctcagagg
     5521 gaccgagccc ggcgcatcgg tcttcgatct catgaatgct atcggcgcac acgatcatgg
     5581 gcgagccttc agaattcttg cgaggaatgt cgagaacggg gaggcccccc tccgtatcct
     5641 tggcgcctta gtctggcaat accggcgtct ttggaagctg aaggagcaac tcaaagccgg
     5701 tgggcgggaa ggcgaggctg cgaggacgtt tcggatggat ccttcgcgag tgcggggatt
     5761 tttagcgcaa ttcccggatg accagatgac tcaagcgttt gagtggttct tggagacgga
     5821 ttcgaagctg aagggcggaa gcggaagtac cccggtccgt gtgatggaag atctcttgtt
     5881 tcgactgtgc gggaagccgt cgaaaccggc atcagcagcg cagcgggtgc aaccggcggt
     5941 accaccggcc tcgcgcccat cgggatcgag accggtgtcg aatgtcagaa cggttacgcg
     6001 gaagcggtga tggcgttcac gcgcacagcg agccgggaca cgcggcgtga ggccgtgttc
     6061 ggtttcaaca cgcctttcgt gaccgctttg ctcagagctg ccgtggcggc acgtaaggtg
     6121 gccttagctt catccggttt cttcgccgcg atcgcatcct gaaccttgcg gaggatgctg
     6181 cgaaccgagc tgatggtcgc gcggttgcgc aagcggcgtt tttcggattg gcgagcacgt
     6241 cgaatcgtgg atttgtggac aacaggcata cggattctcc tacgttcaaa gcttgagctt
     6301 gtaacatagg tgacgaagat aggtcaagga tgctgaaagg atcagcggct tcggggcctc
     6361 ccggtcacca gggtggagtg atgatgacgc gaattgatgc acgagatcgg ttaattgtcg
     6421 cgctggatgt tccttctgcg gcagaggcag aggccttggt tgatcgtatg ggcgatcagg
     6481 tgcggtttgt gaaggtgggc ctggaacttt acacggtggc gggtcctgaa attgtgcgga
     6541 gattggtaga ccgcggcaag cgcgtgtttc tcgatctcaa gttcctcgat attgaggaga
     6601 ccgttcggcg cgcaacggct cgagttgcgg ccatgggcgc gacatttttg acgattcacg
     6661 cgaaccggaa ggcgttgatc gcagcagtgc aggggcgtgg gcagtccgat ctcaagttgc
     6721 tcgcggtcac ggtgctcacc aattttgacg ggaacgacct gcgggatatg ggtattcagc
     6781 ggagtgttcg tgatttagtc acggctcggg ccatgttggc ggctgaaacc gggtgcgatg
     6841 gagtcgtggc ttcaggagaa gagccggaag cgatccgtgc caaggtggga ccgaatttac
     6901 tcattgtcac ccctggtgtg aggccggccg gcaatgctac tgatgatcac gcgcggccca
     6961 cgacaccgac tcaaaccatt gccgccgggg cagactatct ggtgatcggg cgtcccattc
     7021 gtgacgcgca agatcctccc gcggcgacag cagccatcct gaaggaaatg caagcggcgt
     7081 ttgatcatcg gggatagcgg agatcaggtg acttttgagg agcaggtccg aaaagatgtg
     7141 ctcctgcgat tgaggcgatg agggcggttg cgactgttct gcagggtttg ttaagatgcg
     7201 cgttctctgc tgaccttgta cggtgggtgt agctcagctg gttagagcgc cggattgtgg
     7261 atccggaggt cgcgggttcg aaacccgtca ctcaccccaa cgtagccgag gagcagggtt
     7321 cgagtgggga taccatgcct gctggcaata agcagctcgt accgtcagtc ccacatcgtc
     7381 tttctgtggc tggtgtacga ttccacgtgc gatcctttcg tcgctttccc gtccaatgct
     7441 cggtgtatta ttcctgtgat gccttccagg gaagtggtat cgcctggaac ctttccctga
     7501 atgggtggcg cgtcgatggg acacatccgg tagaccctgg aacggtcgtc accctctgta
     7561 tctttcttcc cggtcagcat ccatccgtgt ttgtcgaccg cgcgattgtc cgttggtccc
     7621 gcggccagga attcggcgtt gaagtgaaca cgatcaaggc tgatgagcag gcccgcctcg
     7681 aacagttcgt gacggcgtta gtctaaccta tcctatcctc cttcatggtc tcgatcagtg
     7741 cgtgatcaat aggtgtccgc cttgtcggga acgaaaatct gcttggtcct tgtcagcgaa
     7801 ttgcgtagac tggtccctag tcaaggttga tgcgcgcagt gctcatccct ttgctcacac
     7861 tatggccaaa tcaccaacga cccgatccca ttctcgcgtg cctgtcagct gtttcctcta
     7921 ttatcttggg gagggcctgg ttggaaccgg caaagtgtgc gatctctccg tcaggggttg
     7981 gcggatcgaa ggggataaac ccgtcacggt tggcatgaaa ttgacgttgc gcgtcttttt
     8041 gcccgatgag ccgaaggcga ttgatgtgga aggcgtcacc gtgaagtggg tgaaggaccg
     8101 tgcattcggg cttgaaaccc tgaagttgag tgccgcggct gaagcgcgta ttcacaagtt
     8161 tgtcgaaagc gtgttgaaat cagcagattc ctcccgcgtg gcataggtta atcgagtcgt
     8221 tgcttgtcgt ccggcctcac tgtccagctt gaaaaagaga ttccgtagcg tgcccgcctt
     8281 gtcgctcatc gccgcactcc accgcaccct ctgtttccgt tgatccccca ctttcgggga
     8341 ctatcccaca atctgttgtt tttcatattc tgccgaggcg tttggctgga ggaatgatgg
     8401 aacaacagaa tcgtccgcgg tttatcgtcg aatgtaccgg ctcacgttcc gaggacgggt
     8461 tgctggtctg gaacggccgg atgcttgacc tgtccattcc gggatggtcg cgaaccggac
     8521 tcaagggact gcaagccggg gacctccttc aattgcatct gcatattccg ggagagccca
     8581 agcccctgtc ggtacggttg gctacgatac ggtgggcgag tgagtcgcgg ctcggagtcg
     8641 atcccatact catggatgcc gacgatcagc tgcgattgag cgatttcgtc agtgcccaca
     8701 ccacggtgcc gtcgttcagt acggatcgaa aggaacaaat cgtcatttct gatgccgggt
     8761 aagcagtgcc ctcacctccc tgaacactat tcccctcact cctcgatcgt gtcgaaattc
     8821 cgctgaagcc agattttgaa aatgagcgct ccgatcatcg aaggcggcgt cgctctgcat
     8881 cgatcaggct ctgataggct ctggtagaga tgccctgatg tcaggtggga agccgaattg
     8941 cttgacatcg ttgggcaaga tggtgtctct tagccaagac acattgtcac acacaccagc
     9001 ctttcgccgg tccatttcgg tcatgtcata acagtgttgc tagggcggag gcgtgcgaga
     9061 ttgacgtatg actcctggcc ctcagcttcc tgacgcgacc catacaaccc cgctcacgcc
     9121 tgaaaccgag gcgcaagtcg accaattcgc ccatacggat attttggtcg gtattcccag
     9181 cttcaacaat gtggaaacga tcggccatgt cgtcaaagcg gtcagcgccg gcctggccaa
     9241 atatttcccc gaggcccgtg ccgtcctggt gaattcggat ggcggctcaa cggatggcac
     9301 gcagcaggtg gtctcacagg cggttgtcga cctcaaaact ctctttatcg gcgaccagca
     9361 gagttcgctt cataagatca ttacgcccta tcacggtatc cccggaaaag gcagcgcatt
     9421 tcgcacgatt tttgagattg cccgtcgttt gaaagccaaa gcctgtgccg tggtggattc
     9481 ggatttgcga agcatcacgc cggaatggat cgaactcctg gtgagcccgg tctttgagca
     9541 ggggttcgac tacgtcgcgc cctactatct ccgtcacaaa tatgacggca ccatcacgaa
     9601 cagcatcgtc tatccattga cgcgcaccct ctatgggcat cggatcaggc aaccgattgg
     9661 cggggatttc ggattttcag gccaattggc gcagcattac ctggacaagc acgtctggga
     9721 gtcggaagtc gcaaagttcg gcatcgacat ctggatgacg acggaagcga tcgccagcgg
     9781 ggcgagggtc tgccagagtt ttctcggtgc caaaatccac aaccccaaag atccggcggc
     9841 tgatctttcg gctatgctcg tgcaggtgct cggtgcagtg ttcgcgctga tggaagatca
     9901 ttatgcagtg tggggaaaaa tagatggttc gaacgcggtg ccgttgttcg ggttccagta
     9961 cgaagtcggc gttgagccgg tcaacgtcaa cgtcgatcgc atgatcagca cctttcggca
    10021 aggcctcact gatttgggta cgatttggga ccagattctc gctccgggaa ccaggcagag
    10081 cctgcggcca cttggcacct gttccttaca ggattttcgg attgccgaca gtctatgggc
    10141 gcgggtggtc tacgatgcag ctgtcgccta tcggaatcgt gtcctgcctc tcgatcacgt
    10201 gttgaaagcc ttcacaccct tatatttggg ccggaccgcc tcgtttgtgt tggatactca
    10261 ggggctcacg tcgagcgagg ccgagggccg cattgaagcg ctgtgccagg cgttcgaaaa
    10321 ggataagtcc tacttggtgg cgcgctggaa cgaaccgcat gccggttgag agtcatgaat
    10381 ccatccatct gaaggcaggc tgcagtcgag gccggaggag ggaagtatga aggaattctt
    10441 cgaaaagggg ctccttgatc ctctggagct gatggcgcgg caggtgctcg cggtgttgcc
    10501 gagcctgctg gccatgtcca tcattttctt tgcgggttta gtggtcgcgt ggacggcgag
    10561 ccaggcgctc gagcggctgc tccgcgtggt cgggttggat cggttgtttg atcgactggg
    10621 cgtgaccggt gctttgttgc gaggcggggt caagacggat ccgtctcggt tggtggggca
    10681 agcggcctat tggttggtta tgatttttgc caccgtggcc gcgctgggcg cactgaatct
    10741 ccaaccgatc aacgatttcg ccaagtcgtt tttggcctat gtgccgcatt tggtcacggc
    10801 agggctcatt ttggtagcgg gctggttgtt gtccaacttt gtgtcgcagg cggtgctgat
    10861 cgccgcggtc aatgcgggat tgcctcctgc tcgattagtg gcgacctgct cccggtgggg
    10921 gattcaattg ctagccgtgg ccatggcgct ggagcagctc ggtattgcgc aaaatatcgt
    10981 ggtcgtcggg tttggaatta ctctgggagg gatcgtcatg gccggggcga ttgccttcgg
    11041 attaggcgcg aaagacttgg ccaaagacta tctcgagcgc gggctgtcgg ttcgaactcg
    11101 agatggcgca ccggatgact tacggcatct ctaacggctg agcgttcttg gctgttcact
    11161 gataaaggac atggtatgac gaataagatt tcggatatcg acggcgaggc caaggcgcga
    11221 cgctggctgg gtggggaagc agcggtgccg cggtatcccc accgccgccc gagccggttt
    11281 gtgttcaatc gttcgcttct cgggcttgtg gcctgcgcca tcagcgcggg atttgcagga
    11341 gggctggtgg tcggcctgtt gagccgccgg accaacacgt ctcagcgcgg gcattgatcc
    11401 gtcactcatg tctcatgtgg gttggctgga gcgggatgtc gtagactcga tcgtcagaga
    11461 gtcgggagga gatcaatcag gctgcgattc catccaacgg gtccgactcc atcggcccgg
    11521 atgaggcggg ggagctgcac gtcctggtcg taggagccgt taggtttttg gataagaata
    11581 ggataatcca cgctttccag catgggtaga tcgttgaggc tgtctcccag gccgaccgtc
    11641 acgagagtct gccccttctg ttgctcgagc ctcctatacc attcaatcag ttgtcggctc
    11701 gccattccct tatcgttcgc gcccatcaag tggtagaacc ggcctcctcg cgtgcagcag
    11761 agaccgcgcc tggtcgccgc tgcaagtaga tcctgccacg ctacgacgtc gccatccatc
    11821 acgaagggct cgtcatactc ccgctgcatg gccaactgtg cgtctccagc cgacattccg
    11881 gttaactgaa ccacctccgc cagggtcaga tccccaaatc cccgcaatcg accaccgatt
    11941 tcttgaccaa tctcctttag cgccgctcga agtgtcgcat agggagctcc gagttggaaa
    12001 acctggaaag cttcgcgcga gagtgctccc tcaaggggga agggaaacgt gccgttgggg
    12061 acatagattg ccccgccgtt ttcgacgatg aagggatggt gattgcacaa agagcggcga
    12121 agtggctcca tttccgcacg ggtcttgctg gagaccagga tgagcgtcgc cccgcgatgt
    12181 tcgatggctg ccagggcatc cgaagcgtct ttgtaggagt aggttgtggg gtccagcaga
    12241 ctcccgtcca ggtcagaaaa gatgaggatg cgctgcatat cggtcaaatt ctccctgccg
    12301 cagtatacgg aagtctcccc gccggtcaaa cagggtgtcc aaggtcgatc tctctcgtct
    12361 ctgtgtcaaa accggcacag gaaaatccct aatcaagatc gcacgaaaga atgccgaaaa
    12421 ggtaaggcac atggctctgt caggttcccc atggactcgt cagacccgta gccggtccat
    12481 accattctcg gatgcgtgta aagttatagg gttatacagt gcgatccgat aaaaaacctc
    12541 ccctgaagtc cgataaaaaa gttctcccca aggtccagaa gaagggcagc ctcggctaca
    12601 acaaaaagtc cgcgctgctc gaggacgcca ttacgcaaat gaacgccggc aagtatggcc
    12661 gtgcgtcctc cgccctcaaa gacctgctgg cgctcgaccc cctcaacgct gaagccaggc
    12721 gcctcttcgc caccctccat ctgcggcttg gaagcctcat gagcgccagg acggcgttcg
    12781 aatccctggc ccgggaggcc atggagcggc aggattattg gctggccgag tcgctgttgc
    12841 gggaatacct gacggcaggg ccgcggtacg tgcccttcct ggaaatgctg ggacgcgtct
    12901 acgaggaaaa aggcgacgtc atggcggcag tggccgagta tggcaaagcg gttgaagtct
    12961 tgctggagga tcccgacacc gagaagccga accaccccag cgagcttttc gcacggattc
    13021 gctcgatcgc tcccgggagt ccggtctcgt tccgccttgc cgccatgttc gatactgtta
    13081 cggggcaagt attgcaggct acccctcagc ctgcggttcc tacccaggaa cacgaagcac
    13141 ctcaggctga cggggtagaa gctccggcca gtgctgtcgc gcccatgccc tgggaacaga
    13201 tcgagcccct gcaggctgat gtgcctgtcg ctcaggcccc gtccagtcca tcggacgtgc
    13261 cgccgacagc ggaagtcatc gagccggcgc tgtctgcggc attgccagtc gaggctgtgg
    13321 cagcggttga gtcacaaaag accgagattg atcaaagccc cacgctcgaa tcatcagagg
    13381 tgcgtacgac tctgacgtcg gcggattctg ttgctcctgc tgtgcccgca gttggctcct
    13441 ccgacgagcc gatgaaggcc gcagctgaaa gcgaaccgcc tgctatcgat ccgctccccg
    13501 cgttgagcgg cgaggtggaa tcagcgcctc ccacgattcc tgtggagctt ccgccgacga
    13561 acgtggcgtt ggtcgaggtc ggcgccgatc cgattgcgca tgcgccttcg agcccggcac
    13621 cgatgccgtg ggaccaggtt gaagaggtcg ccgtcgtcat tcctcccgca tctccgagtc
    13681 aggaatcgac ggtttcagac gaagtggctc cgattgccct tgaatctgaa gtcggacccg
    13741 ccgtcaccgt tgaggcagtg cttccctcca tggaactgcc ggcagagacc ggcagccaga
    13801 caacgccttc gacgcctgct cctctgtctg ggaaccaggt tgtagaggag atcatcgcga
    13861 taccgccggc tacgagtgag gtatcggctg tgccagagtc tgcgcagatg ccgacctctg
    13921 aaactgaatg gtcatcggct cctcctagtg atcctgctcc acgcgcaatc gaagtgctgt
    13981 cgcaatcagg cagtcacccg atcgtctcca caccgacgac tccggtgtcg atgccctggg
    14041 atcaggtcga agagaacccc attctcattc ctccggcggc agggtcttcc gcgcccagtg
    14101 tggaggaggt tgcgccggca acatgtgaag agacgccgtt gccgccagtg atcgaggcag
    14161 gaaagtccga tacagagcct cccgcacagc aggcagaggc gcagacggca gccccctccg
    14221 cctccagttc ctcggaaatc acgtcatccg gactgtcctg ggacgaaatt cttgcagcgg
    14281 ttgccgcgat gcaatcatct cctgcgtcgc cccagtcgct tcccagcgcg gaaccgacgc
    14341 cggcaacaga gtccactgtg atggaagagc cggctactgc agtcacggcg ccctcggatg
    14401 ctgtggagat gacggcggtc gctccagacg cgccctcgtc cgaaccctct ttggatctcg
    14461 gcagcgcttc atcgacgttg tcggcgccca tgccctggga acaggtcgaa gtcgaagatg
    14521 tcgccattcc ccgccaagag ccggaaccgg aatttggttc ggccaccatc gcagtcgaag
    14581 aaaccgggaa ctctagtgga atggcattgg attccaccct cgttgtgcct gcggtgccgg
    14641 agccagtgcc gcaagtggag ccgcccactg tcgccgctga atcctcaaca ggcagtgtcg
    14701 aagaatcggg gcgggggttc cgcctgctct cctcggacgg gcaggagccg cacgatactc
    14761 cgctccatca gccggcggat ttacctgagg tgactcttgc ggagaagtcc cccacgccgt
    14821 ccgatcctga gccgacggta gaacttgtga aggaagaatc cgccgccatt cccttgcgtc
    14881 tggtcgaacg cgaaagccta ccctcgcagg ccgcagttga acccgcccct gtcaaagcgg
    14941 agccgatcac ggaattcact ctcgccggaa cgcctgctga atcgattgag acggtcttag
    15001 aggctgcacc tcaagtggag gactcgctgg agttagtcgg tgcgccggag gcgatgatgc
    15061 cttcaggctc tgcaccggta gacgagatcg tcgagcctat tcccttgtct tctgttgaag
    15121 agcagacggt ttccctggct gaaccgattg ctctcgaggc acaggttgaa ccgccggcgg
    15181 agccttcgtc cgaggagagt gagcctgcac cggttcttgc gctggagccg gccgcaaagc
    15241 cggacgagca tgtggtagtt gattctgtgg tgacggccgc cgaggtgtcg ctcacagagt
    15301 tgacggaggt gccaactgct tcagctgaag acgctccgct ggtggccccg cacgagctgt
    15361 cgaccgttgt accggcagaa gagtcgagcg agcttgttca gatcccccag gctcttgagg
    15421 ccgtgcagac agaccagcct gtagacatgg ctgcctcccc ggtccctgct ttggagccag
    15481 aggtgcctgc accggctgtc tccgctccag ctgaggtccc cgcaagcgat ggattacata
    15541 ttttgtggga agatgcctcg tcagcaccga caccgaaacc ttccactgga aatatgctga
    15601 ctcgctggct caaaaagcct gcggagcctg ttgcccctga agtcagcgcc gttcccccgg
    15661 cgactcccgt accggctgaa cctgtcgtgc cggtcgcctc acaggcagtg gaggaatccg
    15721 ttcctgttgc agtcgtgatg gagcagccgt atgaacacat cgagcaagct cctgcggcag
    15781 ttgtggaaga gccgacccat cgtcccacgc gcatccaatc ggccgggggc ggagtatgga
    15841 gccgggtggc ggaagcggcg gcgtctctgg tcggagcagg ggtgtcgaca acgcgatcgc
    15901 tcgttgtcct ggttctggct ttggtcggcc tcgtgctgtt tgtggttggg ggtaccatcg
    15961 ctgcggtggc cctgacatgg ctgatacttg aggagcaacc gaacgcagcc tatcgcacca
    16021 tgacgtcggt gccccaacag acactgcaag actccagtaa aaatggctat ctgctcttgc
    16081 tcggcctcgg agcggccgcg agtcaggatc ctgtgcaggt tggcctggat cgacggattg
    16141 aaggcgccga ccgtgccttc acacatacct gcttgaccgg agaagggtat tcgcccgacg
    16201 aaaggcagag tgcctcggcc gacgcggcgg gcaagtggat gaaagctgcc gatccggcgt
    16261 tgcagatgca aatggatgtg agcggcgcca aaacgtggct gtcgcaagcg gaagtcggcc
    16321 tcagccgcta ccgccaatgg ttgaacaagc cgtttgaaga tgtgggatac ggccagtcga
    16381 tcagtccgaa ctgcgggctc atcctccaga cgcatcgact gtatcttgcc gacggatttg
    16441 cgcaggatgt ggacacgggg ttcgcgcggc tggaaaccga tctcacggcc tggcggacgg
    16501 tcttgggaca ggcgaagtcg ttgccggtga aaatgctggc cagtgcggca atgaatgacg
    16561 acgcgatgat catggacggg ctcttgcagc ggccggatct cgatgaacga tatgtgagcc
    16621 gcatggcgaa attggcgaag ccgttagagc cgtccgaaca gtccctccgt tggccgatgc
    16681 agagccaatt tgtgctcgct accaagacgt tggatgaggc ggtcagccag aaccgggccg
    16741 agacgcgccc gttctatggc gccatcgctg cggcgttgcc gctgccgaag caacgccggt
    16801 tcaatgccta cgccgaatat tatgatgcgg ctggaaaagc ggcagccgac ggacggtata
    16861 ccgacctgcc gaagctctcc cagtttgtgc gtgtggctcc gtacagcgtg agcgacatgg
    16921 tgatgaatcc gattgaaggc ctggtcgggg tggactccat gcccacatgg gaaacctatg
    16981 ccggccgggt tctggaaacc gatgcgcggt tgcggttagt ctcgttgcag gcctggttac
    17041 gccggacccc gccggagcag gacttgctga cccggctggc caaggccggc caggggctct
    17101 acgatccttt taccgggctg cccatgctgg tcaatatgaa aaaaggcgtc ctgtatagtg
    17161 tcggaccgga cctcaaggac aatgaagcgc acgagcgcct tgatctcgtg gctcagatcc
    17221 cctcggtggc ctgggccggc ggcaaaaaat tggccgatac cggcaaagcc aagtagttcc
    17281 gtccgcaatt cttctccccg cctctcaccg aatacgtcgc agtgttgctc ccatcaccgt
    17341 gccgcggtcc tgcccccatc ctgtcaaccg ggattgtctt ctcgcgttat agcctgaaag
    17401 agccgcactc tcgctgtgaa gcaggcaggg tggctgcgag tccggcgcat ccgtttcagg
    17461 actcgtcgtt gcgtcgcgag cggagatttc cttggattct cttgatttca ccgtgtttca
    17521 agtcgaaaat cgggccgatc tcgaccgata ccgggtccat gcggctgctg cctacggggc
    17581 ctcatcggtc gatgactggg cagatagaag acgagcatgg ggatgagcgg ctgccattcc
    17641 cccattgccg ttcctggaca gccaggccac ctgtgcctgg gtgagagggt ggcatctctg
    17701 gatcaaaccc aggcattgga tcggtttctg gccgggattg agcggcgcgc attccgcatg
    17761 gcgcatattg ccacggggaa tgcggacgag gcgttggatc ttgtccagga tgccatgttg
    17821 aagcttgcgg agaagtatgc ccggcggcct gaagaagaat ggggcgcgct gtttcactgc
    17881 attctccaaa gtcgcatccg cgattggtat cggcgggaac gggttcgaaa ccgcctgcgc
    17941 gaattttttc gtggggctca ggatgatgag gacgcggagg atccgctgga gcagattccc
    18001 gattcaagca ctccggcacc ggatggcgag gtgcagcgta agcgggcctg tgcggagctg
    18061 gaagtggcgt tgcgtgcctt accgcttcgt cagcaacagg cgtttcttct gcgcatctgg
    18121 gaggaactgg atgtcgcgca aacggctcgt gcgatggggt gttccgaggg cagcgtaaag
    18181 acccatttgt tccgggcgct gcaggtactg cggcagcgat tgggagaaca ttggccatga
    18241 acgaacgatc ggacgagcag ccggatcctt tatcagccgc ggcgaagcgt cttctcgatc
    18301 accgcctgca ggagctcgat caaaagacgg tgctggccct gcagcgtgcg cgggtgaatg
    18361 ccgtaagccg gtcctctgcg cggcggccct ggcttcaatg ggccgggggg gtggcgctgg
    18421 cctcgatggc cgctctggcg gtgacgatgt ggctgtggca ggcgaatggc gccaaccatt
    18481 cgcatctgct gttggaagat ctcgaactga tgcaatcgcc ggaaaatctg gaattgtccg
    18541 aggacctcga attttacgat tggctggccg atggtactgc cacgacgggg tagtgcagga
    18601 ctgatgctcc tgttcttctt ggccaccgga atagcagagg cggcatcggt accggcacgg
    18661 ccggcagagg agccggaccc cgagttcctt gatttcctgg gtagctggca ggatgaggat
    18721 ggacagtggg tcgatccctt taccatcacc agcgatcaag tgccgcagtc ttccgtggaa
    18781 ccacaaccga atcgcagcgg aatgtcttca gacgctcaga agcctgcgcc agagaagccg
    18841 gcaccgtctg cgaaggatcg ccctcgcagc cccataagga tgcaaaccgg cccatgaatg
    18901 tgttgctggt aggaatcatg gcagtgttca tcgcattgtc gcctgcctgg gcgcaaggtg
    18961 accagaccgg ggtcccatgg agccagctca gtccaggcga gcagcaattg ttgcagcggt
    19021 tcagcgacaa ctgggatcag ctccccccgc gcaaacagca acggttgcgg aatggggcgc
    19081 ggcagtgggg cacgatgacg cccgaagagc gtcaggaagc caagcagcga ttcaaggaat
    19141 ggcgatcgct tcccccggag caacggcagc aattgcgtga gcggtatcaa cagttccgcc
    19201 aattgccgcc tgaacggcgc gaatcggtgc gggacgccag gcgatggttc cggtctcttc
    19261 ctccggagca acggaaggag ttgcgggaaa agtttcagac aatgtccccg gaggaacgtc
    19321 gtgcctatcg gcgggaactt cgccgccagt atggcggcag tggcggcggg caggcgcctg
    19381 ctggagtccc cggtcagtaa tcttccggcg agttcgtagg ccgtgatgcg tcaggtggta
    19441 cgggcgtcag tcgtctcggc ttttcgatga tccttcacat agacgctgcc gggctggctc
    19501 ttggcgattt ttttgagctc ggaggcaatc ttgctgacat cgcccggatg ggtaatggct
    19561 cgatgttcgt tggtaaccac ggcgatggac aggctcatga gagaaaaccg ttcgtggttg
    19621 ccgcgccggt cgacggagtc gataaacccc ttgccgcgat catcgggatc gtagaagtcc
    19681 ggaatcacca aatcgaaccg tttgatcaat gtctgacaaa tggcttcgat cgtggccggc
    19741 gcgctcataa agacaaaatc atccccaccc acatgaccga cgaatccgtc cgtgcccgcc
    19801 agttcactga cgacggtggt gaggatgcgg caggtcacca cgaggacttc atcgcctcgc
    19861 gcatacccat accggtcgtt gaacgatttg aagttgtcga gatccaggta ggcgagggcg
    19921 aacggagcgc cgctttcaat ccgcgccgtg gtttcgtgga ggatcgtgct gttgccgggc
    19981 aaccgcgtaa gcgggttggc atccagcgac cgttccaggc ggctgaggca gaggcgcacc
    20041 cgttgtggta cttcctcggg ccgatagggt acggcaatat agtcgtcgac gccgagcgcc
    20101 gcccaatcca catcattcac ccggctatcc cgaatcagaa tgatcaccgg cagccggccg
    20161 agaaagggat ccgcgcgcaa ctccttcgcg agggtatccg cggaccgtcc attcgtccct
    20221 tccgccgcca acaccaggca ggggggatca tgcaccaatt catgcagcgc tagccgtccg
    20281 gcatcggcca cgacgacgct gaatcctgca cggctgaggc cagccgtcag ccgctcgatt
    20341 tcgacgggat catggtgcag cagcaggata cgtcggttgg aaggcgaccc gctcatagat
    20401 agtcgtcgat ggtttgaaat tcttccgcgg cttgggcgag gctctgcgcg agactttgcg
    20461 catcgaggcc gacgagatcc caggcctgac gggaaagtgg agggatcagg tcgtcgccgg
    20521 ggttgccgca ggcgaggccc ttcaccagca catccgccac atgcacgatg gcggtctgca
    20581 gcttcgcgtc ttgggctgcc gtcggcatat ggtgatgaag aatcggctcg cgcagagtgg
    20641 tcggcagatg ccaggcggtg gcgagccacc ctccgacgtc ggcatgggtc acggcaaata
    20701 gttcctgttc cgtttccatg agcgatcgtg agccgtcttt cctggcggcc gtgatctgct
    20761 ggccgacatc cggccatttg acgtagagca ccaccttacc gatatcgtgg agcagtccgc
    20821 cgacaaacac ctcttcggga ttcttcatgc cggcttttgc gcctagaata tgtgccgtaa
    20881 tggccacccc gagcgaatgg cgccaaaggt cattcatccc ggcgtttttc atcatgtcga
    20941 acgcggtggc gcacagcgtg aggcccttga cgacactcaa ccccaggacg acgactgcat
    21001 gggcgactga ggcgatgcgt gagggaaatc cgtagaacgg cgaattggcc aaccgcaata
    21061 ccttggcgga cagcacctgg tctttttcga tgagggcgcc gatttcctcg gctgagacat
    21121 tggggcggcc gatcatcgac gccagtttct ggacgacatg gggcagggtc ggcaattcgc
    21181 cgacctgctc gatgcgtcgc cggagatcta cgttggccga aggcttcatg tggctggagg
    21241 tgcctcctgg tggtcggcta ccccatgtgt cgtccggagt tgctggcgga tcgcttcacg
    21301 aatctggcat tgaatcgggt catcgctcac aaggcggaag cggtggtcga gctcggcttc
    21361 caactcggca agggtctttc cgctggcatc gccggcgtcg ccctcgacat agacggacgt
    21421 gaggcccagg cgctggagtc gagagagggt tgcttcatcc agttcggcgc cgggaggaag
    21481 gacgactagg cccgtattgt tggtcacggg cttggcgaga atcattcccg gtgtgagctc
    21541 ttcgatcacg acgcgtttca tgcctggctc tcgtgtctct tcgagtccat aagcgggaag
    21601 gccgacaaga gcggttgccc gtcagcacct gaggatagct ctttgggctg tatcggctga
    21661 aacgtgatcg atcttgagcg gaaaacaggt gccgtgtggc cggagggacg gtccgtctca
    21721 acagtctgct tgacaggacc ggggacattc cacagaatcg atcgcatgat gaggctgaca
    21781 gttcatgaag ggagcgggcg atgaagtgtg aaattcttta tccgggggcc tttccaatgg
    21841 tgcgggtgta tctggacgcc agtgagacgg tgaaggctga gtcgggcgcc atggtggccg
    21901 cttcgccgac cattgatatt gagagcaaga tggaaggtgg gtttctcggg gcgctttcgc
    21961 ggaagatgtt gagtggcgag aaatttttct tccagacgtt gcgggccagc cggggtgcgg
    22021 gagaagtttt gctggcaccg acggtgcccg gcgaaatcgt gctgctggag ttggacggcg
    22081 tcaatgagta catggtgcag aaggacgggt ttttggcggg cgccgaaggc gtgaagattg
    22141 aaagcaagat gcaaagtctg acgcgtggat tgctgggcgg ggaggggttt tttattctga
    22201 agatcggcgg gaccgggcag ttggttctca acagcttcgg ggccattcat aaaatcgaat
    22261 tgaaaccgaa cgaagaatac attgtcgata acagccacct ggtcgcctgg agctcaacga
    22321 cgacctacaa catcgaaaag gcctcgtcag gctggattgc cagcttgacc tcgggcgaag
    22381 gtttggtgtg ccgtttccgc gggccgggtg tcgtctatat ccagagccgt aatcccggca
    22441 gtttcgggaa ctggatcaga caattcattc ccgtttcgga gtagcggctg nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcgcctgcg gccgcgttgg ccgccgtttt
    22621 gaacgccccc ttcacaggcg atagttcttc atgaggaaag tccaagccct atgaacgcga
    22681 ctcccaacgc actctgtttc ggtgagggac tgccgggagc cggtgtgccc tgtaccgtca
    22741 tgctggttga agagggtttg cgtgtcagtc ctatgggtga gtctgcgctc gccgaacagg
    22801 tcgtgacctt tgctgacatg gcggttgaag ccggcggatt ggatcacgat cagctggtgg
    22861 tgtcctgggg caccggccca tccgtttgga cactctatgt aaaagatccc gccttgatcg
    22921 tcgccttccg ccgtgcggct ccgcctcagt tgacggccca tctggagcgt gcggcagagc
    22981 aggttcgtcg ggcgcgacac agccatcgcc tcttgtgggg cagtgtgttg ggggtcgtat
    23041 tcggcctggg gctgctgttg tggttcggga cggacctgat ggtcgaatgg gcggtggcca
    23101 gaatccccat cgagtgggaa caaaaattgg gcgaaaccgt gtatcaggat tttctgtcca
    23161 aggagaccgt gctgaaggaa gggcccgcgg tcagtgcagt gcaggaaatg acgcagcggt
    23221 tgacggaaaa aattcccaat aatccttaca aatttcagat ctccgtcgtg cagagccccg
    23281 tggtgaatgc ctttgcgctc ccgggcggct atgtcgtggt cttcaccggg ttgatgacca
    23341 aagcagaaag cggcgaagaa gtggccggcg tgttgagtca tgaattgaac catgtcttgc
    23401 agcgtcacgg catggaacgc atggtcaaga tgctgggact gtctgccgtt gtggggattc
    23461 tgctgggtga ccaacagggc ttgaccggcc tggccaagca ggtgggcatg gagttagtga
    23521 cgttgaagtt cgggcgcgcc caggaaacgg aagcggacgt caccggcatt cggctcttat
    23581 ctgacgcgcg cattgcaccg gacgggatga tccgattttt tgaacggctg tctgaaaagg
    23641 acaaggaacg cgtcgaagtc ttttccacgc atcccatgag cgcggcgcgg gcggcgctct
    23701 tgaaggcgga gttggccgca ttgcccaagc gaacccctga gccgttcacc tttcaatgga
    23761 aaaccattca ggattcattg gcagtgtctg agcctaagaa gtaacgcaag gagcggaatg
    23821 cgcatcggag tcatctcaga tacgcatggg ctgtttgatc cttcgatcac tcgccatttt
    23881 gcaggggtgg agcatattct gcacgctggg gatatcggtc cccgtgatgt cctggcgcaa
    23941 ctggagcgga tcgcgccggt gaccgccgtg tccgggaatg tggatggatt cgatgccagc
    24001 ggcattcctg tggagcagac cattgaactg gcgggccatc gtatcgcggt gtaccaccga
    24061 ctatatgagg gtgggcgact cacaggggac gggatagatc tgctggagcg catccgtcca
    24121 acggtgtgtg tgtatgggca tacccatcag ccgaaggcgg aatggcgaga cggggtcttg
    24181 ttgttcaatc cgggatccgc cggaccgaaa cgctttcgtc tcccgcgcgc gctgggagtc
    24241 ctgctgtttc aaaatggatc aattgaaacg cggcacatca tgttggccga tcgagcggaa
    24301 tgacgagaac cgccgatgcg cggatttttt gctctacatg gtggtggggg ttgtctataa
    24361 tgaaagagaa atttatggac agtcggtcgg gcggagtcat tcacttgtta cgaggaggag
    24421 gacttatgct gagacaaatc atcgttgcgt tgggggtggt ggcggtagca tcacaggccg
    24481 gattggctct ggcagccaat cctgatacgg gacccggctg tggtctgggg aagttggcct
    24541 gggccgatta taaaaaccag aagaacatcg ccccgcaggt gatgatggcc acgacgaacg
    24601 gcacgtttgg cagcgggaca ttcgggatca
//
LOCUS       DAOQEW010000035        24486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_486_length_24486_cov_10.398878, whole genome shotgun sequence.
ACCESSION   DAOQEW010000035 DAOQEW010000000
VERSION     DAOQEW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24486
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_24486_cov_10.398878"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLS35_05865"
     CDS             complement(<1..216)
                     /locus_tag="PLS35_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18216.1"
                     /translation="MGKLNENLQVPLGVEAGLRKFLYDQLQAFARKVNGLSSGTFAAV
                     DGVGTAAPTTGTWAVGDFIRNSSPSEAG"
     gene            complement(216..1667)
                     /locus_tag="PLS35_05870"
     CDS             complement(216..1667)
                     /locus_tag="PLS35_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18217.1"
                     /translation="MIGFVGFAPDLDPSTPGVITDCIQLIPNERGMEAAPSAVAAVSG
                     LGNLAAECRGAAVLTNTSGTRRTFAGTQTKIYELASGTWSDVSGMTFTGSSENRWCFA
                     QFGNVALASNDTEQIVASTSGTFAAVSGAPKARVILSIPNFVVALNTDSSAYGDAPDR
                     WHTSAFQDYSSWTANVTTQATTGRLIGNGGELTAGFPFGNGLVAYKAREMFLGQYVGP
                     PVVLQFDRVPGEQGCVGPEAVDDIGGAHIFVGEDNIWMYDGSRPVPIAQDTVRQWFFN
                     DLNATYKYRTIVKYDRNNGRVWIFYPSTTSSGNPDSALVYHLATKRWGRANRNIEAAV
                     NFVSPGITWDTLSSLASTWDTLPSIPWDSQSWQASGRALAVFNSTNGLVTLTGSGEDS
                     GMTTGDFGDNEQVSFVDKVRVRFFTQPTTSTVTGQTRMELGGSSTTAGSGAFANNKYD
                     IRQSGRWHRFSFLFTGSHEVSGIDVPLKRAGKR"
     gene            complement(1664..2260)
                     /locus_tag="PLS35_05875"
     CDS             complement(1664..2260)
                     /locus_tag="PLS35_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18218.1"
                     /translation="MATDYSTLKTEIADFAERNDLSSVIDTFIDLCESEMQRELKLLE
                     FETTGTVTVTSGSGTLPTGFIGARALSWDGDEDRILRYITPDRLNTVNASNPSLVHYY
                     TITGDTIKFANDASGTLNITYMARFTPLSNSNTTNAIITNHPAAYLYGSLKHLSVYLK
                     DAEGAMGYDSLFKAELAAIKKDTQDRRYAGASLAVVCG"
     gene            complement(2236..2778)
                     /locus_tag="PLS35_05880"
     CDS             complement(2236..2778)
                     /locus_tag="PLS35_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18219.1"
                     /translation="MDQSPIRLNLGCGPKILSGFVNVDLPGNWSGQKPDIEADVTKPL
                     PLPDNYADEVHAYHLFEHLNRWEAPGILKDWVRILKPGGLLVMEMPCLDKIIANYAHA
                     LIEGETPEPRLTILGLFGDPSYKNEQMMHRWCYSIAELAAGMQMLGLEEITPEEPQTH
                     QPQRDMRMTARKPWPRIIQP"
     gene            complement(2754..3482)
                     /locus_tag="PLS35_05885"
     CDS             complement(2754..3482)
                     /locus_tag="PLS35_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18220.1"
                     /translation="MIPAQELKKHGIEQGNDWLVIGKHGWDWKKQTAGFKRVCFDVCD
                     DHFNTPSGDFYREAIQRADLVTSNSQEMARVVKEKTGRESIVIPDPYEQPERKARVHD
                     HLLWFGHQSNLGDLLPWLDRLDNLEVVSGVRVNGVVQWSPEAMDAAFERAGLVVIPTG
                     KSMAKSGNRAIESLRRGLFVVTGYLPAYADLGIYIGDISDGVKWALSHQDEVIYRIKS
                     AQDYIRNEYSPERIGQLWIKALFV"
     gene            complement(3536..4060)
                     /locus_tag="PLS35_05890"
     CDS             complement(3536..4060)
                     /locus_tag="PLS35_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18221.1"
                     /translation="MNGQVLLFLRKIGPQAGMKLIEIGSMDVNGSPRETFKAAEEYVG
                     IDFRPGRGVDKVMNAEQVHENFPAGYFDTVLCCETLEHCENWKEVVRSMWAILKTNGK
                     CVITTPTRKKGRHNYPNDYWRWELDDYKKMFAAQNIIRCEPVGTAGIGVIVEKVSDEL
                     DLEVTPHQIPPPKK"
     gene            complement(4057..4641)
                     /locus_tag="PLS35_05895"
     CDS             complement(4057..4641)
                     /locus_tag="PLS35_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18222.1"
                     /translation="MIPLALNLLRDYQETHTDGSNAFIYSRFLVPHLQGYKGWAIFCD
                     GDMLCRDDIAKLWAMRDDQYAVMVVKHDYETKHKTKYVGTGLETVNVTYPRKNWSSVV
                     LWNCGHPANRVLTPEYVMGATGRKLHRFEHLLDGEIGDLPKDWNWLAEEYPVNEDAKL
                     VHYTLGVPAMKAYAESQHAKEWFEAFYGTQYIDT"
     gene            complement(4610..6061)
                     /locus_tag="PLS35_05900"
     CDS             complement(4610..6061)
                     /locus_tag="PLS35_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HNI18223.1"
                     /translation="MSEWDRLSKLIDEDPDEAARQAAANLDKNPDDALSLFVIAETYS
                     RAERFGMAANVYKRICEIRPERAEPWNNLGMCYAGMGMQIKAREAFFQAYNRNKESSM
                     FSANLGMTYFSEREFKEAKKWCEKALKLDPESRAAKNTLGMTYLSLGEWDKGWELYGA
                     SVGGKFRRHIQYLEEPMWDGTPGQHVVFYGEQGLGDEIMYASCVEDAKQVCASVTIEC
                     DARLTNLFKRSFPGVTVHGTRRKNEVEWPAKQRIDASLPVGQMPQFFRKSTKDCPGKP
                     YLVADPERRVQWRALFDSLGPKPKIGICWSGGSKHNKPKERAIGLEAMKVLIESVDAE
                     WISLQYKDPTAEIEASGLPIRHYKRACETDDYDDTAAMVAELDMVIGVHTTAHHLAGA
                     LGVPGVILVPSRTIWIYCLPDGSMPWYGSATLFKQREAEPWKETIKRLINDPCLHRLR
                     SPRSSGVPHLQRVGYQECHKANPVDTARAELAA"
     gene            complement(6058..6387)
                     /locus_tag="PLS35_05905"
     CDS             complement(6058..6387)
                     /locus_tag="PLS35_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18224.1"
                     /translation="MRIFESSFDPLTGIKTTIGTEDDKLVIKYDGDVEPSLDYTNDLR
                     KSDDYTSIGIKKNWWHCFHIPPVIAMKMIQEDGFDPYMSHAKETFAFIRRNKEKYGHC
                     LTTRGKF"
     gene            complement(6617..7594)
                     /locus_tag="PLS35_05910"
     CDS             complement(6617..7594)
                     /locus_tag="PLS35_05910"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HNI18225.1"
                     /translation="MAQVAGTTDTYDLIGLAEDVEDVIWNISPTETPCLSMFAREKVT
                     NTLHQWQTDSLASATTNRFVEGDDSTYATASPTTMLSNYTQISKKTVMVSGTADAVRK
                     YGRKEEFAYQIAKKGRELKRDIEYAIVQNQGSSAGSTSVVRSSASLESMIAGNRILAG
                     STNTTGTTAGYSSGTWTAPVDGTTAALTETTFVQALDAAWQDGGDPTTVMCNSSQKRT
                     IATFTGAARYAGVSVNQNAGRTSQAVVIGGVDGYISDFGEHKIMLNRYMRTRTVLFLD
                     PGYWATGWLRPIKFEERAKTGDATRGELLCEWTLIGRNPDSSAKVQDCS"
     gene            complement(7596..8576)
                     /locus_tag="PLS35_05915"
     CDS             complement(7596..8576)
                     /locus_tag="PLS35_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18226.1"
                     /translation="MDQTTPETEVSSGITEEQAVAQILGRWGVTDEPKAEPAQEEPQE
                     EAQAEQTEQVDAEASEESSEEPGQEDEAEIDVAGEKFKVPAQFKDTAQRIQAKAKEVE
                     AGATRKFQEAAELRKSAETQLEAVKQLQKLAHAHADLLADHKMVAKRLQQLESIDIQS
                     TDSDTLTRLNAEYNQLQAAQRRIEAQLGKSDEEMRAEETKAVQAKIAHANETLKTRVK
                     GWGPEMAKSLTEYAISRGATPEVVAGITEAWMVEVLADAAYGRQMREHKSTVDKRVVQ
                     TPKTLKPGASGKTTAPVMKAEQAMKRVQQTGSIQDAAAALLMRSQVKRNR"
     gene            complement(8647..8922)
                     /locus_tag="PLS35_05920"
     CDS             complement(8647..8922)
                     /locus_tag="PLS35_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18227.1"
                     /translation="MTDKTEEAIRRGARAKAMLDEPLMTEAFAHIEAECFRLFQGLSP
                     TDIDGMQQVKAMHYYLGKFKTFLEKAVKDGVLAKAQLEPKQYRPKSY"
     gene            complement(8919..9455)
                     /locus_tag="PLS35_05925"
     CDS             complement(8919..9455)
                     /locus_tag="PLS35_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNI18228.1"
                     /translation="MSTAFQSDAFQPNAWQILAGTAETPVEVPNVVGLTQEDGTTTLE
                     AEGFVVVVSETFSPQPAGTIIEQNPTGGSFALSGSTVTILVSKGSASQRAGGRNYIIR
                     GKRYRNLTNEELAYLLARDMIDVQRADIKVSYKGKKPHMVSKAQWASLETTLKGLEQF
                     LAPPFDEDDEESAAMLLL"
     gene            complement(9457..10032)
                     /locus_tag="PLS35_05930"
     CDS             complement(9457..10032)
                     /locus_tag="PLS35_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18229.1"
                     /translation="MAKGQVLRAVELPATAVEPVPSEPLRLDVGCGKTKMDGWEGIDS
                     IDFGQKHVHDVRKGLPFADNSVSEVRSSHFVEHLTGQERIAFFNELWRVMKDGATAQI
                     VTPNWSHACAYGDPTHQWPPMSQWYPLYLHKEWRANNAPHVGYTCHFDHVVAGSWDQS
                     IEGRNPETKSMMMNNYTNAWRDLIVTLTARK"
     gene            complement(10033..10431)
                     /locus_tag="PLS35_05935"
     CDS             complement(10033..10431)
                     /locus_tag="PLS35_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VII domain-containing protein"
                     /protein_id="HNI18230.1"
                     /translation="MPFASREDYLAYHKRYYEANKHKGWNKHLESETPLQRQERLAEM
                     RAYQLKRNFGLTTEQYDQMLADQGGKCAICLQEPRAKAGRHNKTASLAIDHCHKTGKV
                     RKLLCTTCNLRLGWFERYKDAVSSYLHEEN"
     gene            complement(10436..11170)
                     /locus_tag="PLS35_05940"
     CDS             complement(10436..11170)
                     /locus_tag="PLS35_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18231.1"
                     /translation="MKPQIVVPEYGKHNEDLEKTAVRLREDSSWKKLDTIMLFPAGQQ
                     IPAKIVHSWMNLYAPPNNGFVRMPTVAALNMEVGEAFSQSIEWILQHPQLSKFKYLLT
                     IEHDNAPPPDGLVKLLAKMEAHPEYDCIGGLYFTKGPGGVAQIWGDPRDPVNNFRPQI
                     PRDNELVECCGTGMGFNLWRMDLFRDPELPKPWFKTQTEGGISTQDLYFWNRARPLGY
                     RCAIDCSVLVGHYDLEGKFGPPDTMW"
     gene            complement(11179..11733)
                     /locus_tag="PLS35_05945"
     CDS             complement(11179..11733)
                     /locus_tag="PLS35_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18232.1"
                     /translation="MLVANHDAGAGAVFSHNKAGSITTIGAGFDNFGTSIGSRIARLE
                     NNPGGSGHTFTMTLTGGGHLAVVEIAGAASASYDVSDQVADTSTPYDSPSVSTTQDAE
                     TAIGYFVSESTGTAHVAGNGFTSLLYSEDYWNSCVAYKSLSVAGSVNSSFTCTGTPAG
                     QVYVATFKDAAAAGPMNVTAWLVA"
     gene            complement(11857..13326)
                     /locus_tag="PLS35_05950"
     CDS             complement(11857..13326)
                     /locus_tag="PLS35_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18233.1"
                     /translation="MAYYLPWLSNASGGSGSAYDSAAQDAYFDGLYSTALSGLTYSST
                     KYVSTTGSNANNGNSLGAAYASVDYAVSQVSANALILVADGTYTIGTSGWLNDTTGNA
                     PPSGTGANFTVIRAINPGSVTLSQSSQSYYGSILRLTAGSRIWVDGFDFIHDTQHDDE
                     AIFNFGADTSRITRCMFRRRQSGTYGATFYLGTGCLMQDCALYGAGRYMCNTGSNSAD
                     ATAGTNVFRRVVARLDWAFCDQPVATFAHYGSDNGSWVDSKQTLWANCVDIDGPAVEN
                     QASFGFKWGSFYHPKHQRDIRHKGCIVLNAGCDLSSFRTDNIGSPTHSAENCSIWDND
                     NNTQAGQTPNGYSVSAGSMTVTNCFGGWILGSDTSGTTNSNNRFTDSPTSPQNIVQRA
                     GSAGAEILYCHGAFLSHWGETNFDTVTTDPLWPWPYEARIKAWMDTQITKPTGHFPAS
                     PTSSRQSFTGTSINGDEMTLSRRVWEAAGAAIPDLTTVY"
     gene            complement(13326..16097)
                     /locus_tag="PLS35_05955"
     CDS             complement(13326..16097)
                     /locus_tag="PLS35_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18234.1"
                     /translation="MAVSHVKSNPVANMTGTVTVFNSAGVTTTAAATDLVRPLDWNSA
                     HNQFMTIGGNTVGASTLSGTNIVFEGAGAVTLSGNADTLVFSAPVQTAFVLSNSNGLT
                     FGTNVSTVTASHNGLTSQSNQAYSAGNGSAAFQTLTFANSNGVSFSTGTQGLFGTVKT
                     DYASSNHSHGNPTLALTNLSGTTASNSAGLTLSLSAAAAGGAATATLYATGNTTQSST
                     GTMALSSMIVRGAGGISAGISNGSIVVSGPDLTSLAATGALSASSNGSTISLGVGTVT
                     VSAVSNTTQNSSGTVNLNALQIQGAGVASVGLSNGSVVISVPSGGGAGDGGNVIAAGT
                     QTGTSLGTIKFENSNGITFGMSGSTQVTASHNGLTTARASNDAIGLNTAQTNVTWTVN
                     SSGLSLNAAGYAGTGTTFNGTNASASMTLNSNGLRLDLSAANGGGGVNTGTFYATGNT
                     TQSSSGTIALSSIIVQGTGGVSAGVSNGSIVVSGPAFTSLSATGALSVSSNGSTISLG
                     VGTVTVSATGNTTQASSGTVNLNGLILQGTGGASVGISNGSIVISGATGGAGGGAAIS
                     AGANSQSTGTVNFANSNGITFGLSNNGTMTASYNSTQFAGTGTTFAGANISASMTLNS
                     NGLNLSASVAAPGAAAENNNINLLGANTAGNTTASGSTIGLSGLNLTLSGTNASQVVI
                     SAPATSSLVGVGGISISTNGSTISISQVPSSRYVNENAVTTTGLAADRSSVSIVPFMI
                     QHQLDMSNMRCLISMSGQTTTNNSTNGAAISISGVLYSLNGSSLSSILSGSNTYTGTW
                     SSNATASVFGARALTMNFNNTTRLAPGEYWWAVHVSSATTAANTSGTSNLTHAFTMGI
                     GAANLTQMLGADPWGQATAATRGVSVGLGILSTGATRASIAISEIQQTGSRAQAAKLW
                     LDMRNYSVW"
     gene            complement(16097..18427)
                     /locus_tag="PLS35_05960"
     CDS             complement(16097..18427)
                     /locus_tag="PLS35_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18235.1"
                     /translation="MKTESLLAAIERHETLADSYGNLSEERTKALDYYLGNPMGNEVQ
                     GRSQVISRDVWDTVEWIKPQLSDIFCGGDQVVMFNPTGPEDVAAAEQETEFVNWVITE
                     KNDWFTVWYSWAHDALLQKTGYVIAYWDEREDRTKEKYEGLTEDEVALLSQDPSVQIV
                     KLEQEEEGFEVELERVKEYGCVKIENVPPERVLVSHNARGLNLQDPRLDFLEYWEYKT
                     ISDLRDEGFEVDDDLQDDADSTQQWEESLRDDFNPFRNNEGEESSPAARRLRVRNVWI
                     RYDSNDDGRTELRRVVVVGKTILEDEECDLVTLVAKCPTPLPHQHVGLSVADAVTDLQ
                     DIKTALLRGTLDNQYLSNNGRFAIDVNQVNLDDMLVSRPGGVVRVDGPVQNAIAPLIQ
                     GNPAMDSVPLMEYVDRISQKRTGVNEQAQGLDPNTLNKTATGAQMLMSAAQQRIKFIA
                     RIFAETGVKALFQVVHALTLKHARKAEVVRLRNQWVVVDPRQWVKRADMSIAVGLGAG
                     DKPQQIAFLTQVLQIQQLAMQVGLANPSKIYNALKRLTQAAGYKDHNEFWADPQVEPM
                     PDPGPPPEVLKEQAKAQAQMMIEQGKAQVQLELARQQAQIEMQKLQAQLQLQESNDMR
                     DAERERLKAQFDAQLEQQRLALEEWKAQLQADLGKYTNDQDNNTKLTIAGLNAQTQSQ
                     SEQLKIGHDAAQKQADRQSQAELQDMAKKPDKQTGTQIQSMQKALDQVAGALKQLTDM
                     QKQVAEGQKSIEQAVKAKRVLVRGPDGRPSHSEIAQ"
     gene            complement(18599..19912)
                     /locus_tag="PLS35_05965"
     CDS             complement(18599..19912)
                     /locus_tag="PLS35_05965"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNI18236.1"
                     /translation="MTTVQIQLPPKLIPVFEGKADVRGAYGGRGSGKTRSFAKMAAVR
                     GYIHGMAGDRGIILCARQFMNSLDDSSLEECKRAIEEEPFLASYYEIGDRYIKSKDGR
                     IAFSFSGLDRNIMSIKSKGRILLLWVDEAEPVTADAWDIVEPTLREEGGDWNAELWVT
                     WNPRNKHAAVESRFRQSTDPMVKCVELNWKDNPAFPDKLERQRQRDFKARPDQYAHIW
                     EGDFQSIVDGAYYAASLNKAKADGRIGRVSPDPLMTYRLFCDIGGTGAKADAFAMWVS
                     QFVGREVRVLDYYEAVGQPLDAHLAWCRERGYTKDRAQFWLPHDGATQDKVYAVSYES
                     ALKQAGYKVTVVPNQGKGAAKARIEAGRRLFPSMWFNEDTTSAGRDALGWYHEKKDPE
                     RNVGLGPEHDWASHGADAFGLMAVAYEEPNSKKFAPIKYPDMGIV"
     gene            complement(19909..20154)
                     /locus_tag="PLS35_05970"
     CDS             complement(19909..20154)
                     /locus_tag="PLS35_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18237.1"
                     /translation="MAARLNKLHQEDIRKKIQATQLINVLQKHALTGDGDLSMTRMKA
                     IEILLRKSVADLSAVTVSGDSENPLQTVHRIELTALK"
     gene            complement(20540..20917)
                     /locus_tag="PLS35_05975"
     CDS             complement(20540..20917)
                     /locus_tag="PLS35_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18238.1"
                     /translation="MSRYVDFNAVDPKHQTMDARLRNWSIWANGRDGLGFAPCAPMFR
                     MYRSTEQWEDSANKAPAVDQLDAQRVQKGVSALPEKHRIAISWNYMSRSHPRKAAKAL
                     GESLEGMKHLIDAGRQMLCNRGV"
     gene            complement(21125..21358)
                     /locus_tag="PLS35_05980"
     CDS             complement(21125..21358)
                     /locus_tag="PLS35_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18239.1"
                     /translation="MNASEVVHAIQHYCPEAKDSSWEEHRGMGYFKTTLHGSRRVITV
                     DLSGWNYMLGVAIHGDENSFGEVLAEIIRGLQS"
     gene            complement(21355..21600)
                     /locus_tag="PLS35_05985"
     CDS             complement(21355..21600)
                     /locus_tag="PLS35_05985"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNI18240.1"
                     /translation="MQALRAHGAYVQDSSRMGGGFPDLVCGYAGKTVLIEVKRPGGSI
                     EKKLTEHQREWISRWCGGKVWLVSTPEEAIQALEESV"
     gene            complement(21639..21809)
                     /locus_tag="PLS35_05990"
     CDS             complement(21639..21809)
                     /locus_tag="PLS35_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18241.1"
                     /translation="MRDEMQEAGLGVDYIPTEPGSLEEDLHNVEAMGSWIVYAVCCGI
                     FAFVFGTALGWW"
     gene            complement(21823..22080)
                     /locus_tag="PLS35_05995"
     CDS             complement(21823..22080)
                     /locus_tag="PLS35_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18242.1"
                     /translation="MMAYETRDNSGRLFKNEDKKSDKHPDYSGDALIDGQAYFMDAWL
                     KTAESGRRWMSFSFKPKQQKQQSAPQKQQRRSRDEDDSIPL"
     gene            complement(22077..22325)
                     /locus_tag="PLS35_06000"
     CDS             complement(22077..22325)
                     /locus_tag="PLS35_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18243.1"
                     /translation="MSVGRGIKHSAQVPIVAFFAANPDEEMSAPDVTIKFGFSDRRTA
                     YEALKCLTRDGYLKPTIKTHPRLWRAGPKLLELIGKEE"
     gene            complement(22322..22501)
                     /locus_tag="PLS35_06005"
     CDS             complement(22322..22501)
                     /locus_tag="PLS35_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18244.1"
                     /translation="MSSDDIKRPKDTAFRYAGEGLPIWPLCFFFHQRKERSGGKMRGP
                     LRNLFSCKACEEKRK"
     gene            complement(22498..22767)
                     /locus_tag="PLS35_06010"
     CDS             complement(22498..22767)
                     /locus_tag="PLS35_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18245.1"
                     /translation="MRKVPFTDRAAQMIVVELQRIKDAGHCPNAALDQSTLMGWRSVW
                     PAKEKYIERAPTAGKNEWLQEHDKREFTAPPHELKELAKKLRRVA"
     gene            complement(22827..23300)
                     /locus_tag="PLS35_06015"
     CDS             complement(22827..23300)
                     /locus_tag="PLS35_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18246.1"
                     /translation="MKLTLDNLHHYATEEGDCLLWNLGCNGDGNPIARVDCKCVKVRR
                     YVFETLLGKPVHKNKRVTTKCCNPLCISPKCLRAMTYGEINRKTIKAGRRIVDSESMR
                     RAVIKSGQAKLHEHVQAIRLSDEPSRVLAQRYGVKPSTIRDVRFGRTWGSDSWVR"
BASE COUNT         4505 a         7328 c         7101 g         5552 t
ORIGIN      
        1 gcccgcctcg gatggcgatg agttgcgaat gaaatcaccc accgcccacg ttcctgtagt
       61 cggagccgct gtgcccactc catccacagc cgcgaacgtg cccgacgata gaccgttgac
      121 ttttcgggca aaggcctgga gctggtcata gagaaacttt cgtagccccg cctcaacccc
      181 aagcggaact tgcaggttct cgttgagctt gcccattacc tcttacctgc tcgcttcaga
      241 ggtacgtcaa tgcccgacac ctcgtgactc ccagtgaaga ggaacgagaa gcggtgccat
      301 ctgccagatt ggcggatgtc gtacttgttg ttagcgaacg cccccgatcc cgctgtggtg
      361 gacgatcctc ccaactccat gcgggtctgc cccgtcacag tggaagtggt gggttgggtg
      421 aagaaccgca cccgcacctt gtccacaaaa gagacctgtt cgttgtcccc gaagtccccc
      481 gtggtcatgc cggagtcttc gccagaccca gtgagagtca cgagcccgtt ggtggagttg
      541 aagacggcaa gagcccgacc agaggcctgc caactctgcg agtcccacgg gatgctcggc
      601 agcgtgtccc acgtagacgc gaggcttgag agcgtgtccc aagtaatgcc gggactgacg
      661 aagttgactg ctgcttcaat gttcctgttc gccctgcccc accgcttggt tgcaagatgg
      721 tagacaagag cggaatcagg gttgccgctt gaggttgtgg acgggtagaa gatccatacc
      781 cgcccgttgt tgcggtcgta cttgacgatg gtccggtact tgtacgtcgc gttcaggtcg
      841 ttgaagaacc actgacgcac ggtgtcttga gcgatgggga caggtcgaga cccgtcatac
      901 atccagatgt tgtcctcacc gacgaagatg tgcgccccgc cgatgtcgtc caccgcctca
      961 gggccaacgc atccttgttc acccggaacc cggtcgaact ggagcacaac cggagggcca
     1021 acgtactgac cgaggaacat ctcccgggct ttgtaggcca ctaggccatt gccgaacggg
     1081 aagcctgcgg tcaactcacc accgttgccg atcagccggc ccgtagtggc ctgcgtcgtc
     1141 acgttggcgg tccagctcga gtaatcctga aacgccgagg tgtgccagcg gtcgggagcg
     1201 tcaccgtagg ccgacgaatc cgtgttgagg gcaacgacga agttcgggat gctcaggatc
     1261 acccgagcct tcggagcgcc agagacagcc gcgaaggttc ccgaggtgga tgcaacgatc
     1321 tgctccgtgt cgttggaggc aagagccacg ttgccgaact gcgcgaagca ccagcggttc
     1381 tcactggagc cggtgaacgt catccccgac acatcggacc acgtacccga cgcgagttcg
     1441 taaatcttgg tctgcgtgcc agcgaacgta cggcgagtgc ctgaggtgtt tgtgagaaca
     1501 gcagccccgc gacactctgc cgcgaggttg cccagcccag acaccgcagc cacggccgaa
     1561 ggcgcggcct ccatcccgcg ctcgttgggg ataagctgta tgcaatcagt gatgacgccc
     1621 ggagtgctcg gatcgaggtc cggtgcgaaa cctacgaagc cgatcatccg cacaccacag
     1681 caagagaagc cccggcatat cgccggtctt gggtgtcttt cttgatcgct gcgagctcag
     1741 ccttgaacag cgagtcatag cccatcgcgc cctctgcgtc cttcaggtac acagacaagt
     1801 gcttgagcga tccgtacagg tacgcagctg gatggttagt gatgatcgcg tttgtcgtgt
     1861 ttgagttcga cagcggcgtg aaccgggcca tgtacgtgat gttcagagtc cccgaagcgt
     1921 cgttggcgaa cttgatcgtg tcgccagtga tcgtgtagta gtgaaccagc gacgggttcg
     1981 aggcattgac ggtgttgagg cgatccggcg tgatgtagcg aagaattcga tcttcgtctc
     2041 catcccacga cagcgcacga gccccgataa acccagtggg gagagtccca gacccggaag
     2101 tgacagtaac cgtaccggta gtctcgaatt ccaacagctt caactcgcgc tgcatctctg
     2161 actcgcagag gtcgatgaac gtgtcaatga ccgaggacag gtcgttgcgc tcagcgaagt
     2221 ccgcaatctc ggttttcagg gttgaataat ccgtggccat ggcttcctag ccgtcatccg
     2281 catgtcccgt tgtggctgat gcgtctgcgg ttcttcaggg gtgatttcct caaggccgag
     2341 catctgcata cccgcagcta gctccgcgat ggagtagcac cagcggtgca tcatttgctc
     2401 gttcttgtat gatgggtctc cgaagaggcc cagaatggtc aggcgaggct ctggcgtctc
     2461 gccttcgata agagcatggg cgtagttggc gatgatcttg tcaagacaag gcatctccat
     2521 caccaacagg ccaccaggct tcaggatccg aacccagtcc ttcaggatgc cgggagcttc
     2581 ccagcggttc aggtgttcaa acaggtgata cgcgtgtacc tcgtctgcgt agttgtccgg
     2641 gagaggcagc ggcttggtaa cgtccgcttc aatgtctggc ttctggcccg accagttgcc
     2701 cggaaggtcc acattaacga agcctgacag aattttgggg ccacacccca agttcaaacg
     2761 aatagggctt tgatccaaag ttgtccgatc ctctcggggc tgtactcgtt gcggatgtag
     2821 tcctgagccg acttgatgcg gtagatcacc tcatcctgat gactcagcgc ccacttcacg
     2881 ccgtccgata tgtccccgat gtagatgcca aggtcagcgt aggccgggag gtagcccgtt
     2941 accacgaaga gcccacgtct caggctctcg atggctcggt ttccagactt cgccatgctc
     3001 ttgccggtgg ggatgaccac caatccggcc ctttcaaatg ccgcgtccat ggcctcggga
     3061 gaccactgca cgacaccatt cacgcggaca ccggagacaa cctccaggtt gtccagacga
     3121 tcaagccacg gaagcaggtc gccgaggttc gactgatgcc cgaaccacaa caggtggtcg
     3181 tggacccgtg ccttcctttc gggctgttcg taggggtcgg gaatcacgat ggactcccta
     3241 cccgtcttct ctttgacgac ccgcgccatc tcctgcgagt tggaggtcac gaggtccgcc
     3301 cgctggatgg cttctcggta gaagtcaccg gacggggtgt tgaagtggtc gtcgcagaca
     3361 tcgaagcaga cccgcttgaa gcctgccgtc tgcttcttcc aatcccaccc gtgtttgccg
     3421 atcacgagcc agtcgttgcc ttgctctatg ccgtgcttct tcagctcttg agcaggaatc
     3481 aacgatcggt agcgactgga ggccagtgaa gacccccaat ccttgaacgt gacgttcatt
     3541 tcttcggggg cgggatttgg tgcggagtca cttcgaggtc tagctcatcg ctgaccttct
     3601 ccacgatcac gccaatcccg gcggtaccaa caggctcaca gcggatgatg ttctgggccg
     3661 caaacatctt cttgtagtcg tccaactccc agcgccaata gtcgttggga tagttgtggc
     3721 gccccttctt tcgagtcggg gtcgtgatga cgcacttgcc gttggtcttg aggatcgccc
     3781 acatgctgcg gactacctct ttccagttct cgcagtgctc caaggtttcg cagcacagaa
     3841 ccgtgtcgaa gtagcccgca gggaagttct cgtgaacctg ctcggcgttc atgaccttat
     3901 cgacgccacg accaggccgg aagtcgatgc cgacatactc ttcggcggcc ttgaacgtct
     3961 cacggggtga accgttgaca tccatggagc cgatttcgat cagcttcatc ccggcttgtg
     4021 ggcctatctt tcgcaggaac aggaggactt gaccgttcat gtgtcgatgt actgtgttcc
     4081 gtagaacgcc tcgaaccact ccttggcgtg ctgcgactct gcgtaagcct tcatcgcggg
     4141 cacacctagc gtgtagtgga ctagcttggc gtcttcgttg acggggtact cctcggcgag
     4201 ccagttccag tctttcggga ggtcgccaat ttctccgtct aggaggtgtt caaagcggtg
     4261 cagcttcctg cccgtcgcgc ccatcacgta ctcaggcgtg aggactcgat tggctgggtg
     4321 gccgcagttc cagagcacga cacttgacca gttcttgcgt gggtatgtga cgttcaccgt
     4381 ttccagcccc gtccccacgt actttgtctt gtgcttcgtc tcgtagtcgt gcttgaccac
     4441 catcaccgcg tattggtcgt ctcgcatggc ccacagtttg gcgatatcgt cgcggcagag
     4501 catatctccg tcgcagaaga tcgcccatcc cttgtatccc tgaagatgcg ggactaggaa
     4561 tcggctgtag atgaaagcgt tagagccgtc tgtgtgcgtt tcttggtagt cacgcagcaa
     4621 gttcagcgcg agcggtatca actggattgg ccttgtggca ttcctgataa ccgactcgct
     4681 gaaggtgtgg tacgccgctg cttcgcgggg atcgtaaccg atgtagacag ggatcattga
     4741 ttagcctctt gatggtttct ttccacggct cggcttcgcg ttgcttgaag agcgtcgcag
     4801 agccatacca gggcatcgaa ccatcaggaa gacagtagat ccagattgtt cttgaaggga
     4861 cgaggatcac tccgggcacg cccagtgcgc ccgcgaggtg gtgagcggtt gtgtgcacgc
     4921 cgatcaccat gtccaactca gcgaccattg ccgccgtgtc atcgtagtca tccgtctcgc
     4981 acgcccgctt gtagtggcgg atgggcaatc cagatgcctc gatttctgct gtcgggtctt
     5041 tgtactgaag gcttatccac tccgcatcga ctgactcaat gaggaccttc atcgcctcca
     5101 ggccaatggc ccgctctttc ggtttgttgt gcttggaacc acccgaccag cagatgccga
     5161 tcttcggctt gggaccaagc gaatcaaaca aggctcgcca ctgcacccgg cgttcggggt
     5221 cagcaacgag gtaaggcttc ccggggcagt ccttcgtgga cttgcggaaa aactgcggca
     5281 tctggccgac aggcaaagag gcgtcgatac gctgctttgc gggccactca acttcgttct
     5341 tcctgcgcgt gccgtggacc gtcacaccgg ggaatgagcg cttgaacagg ttcgtcaggc
     5401 gggcgtcgca ctcaatcgtg accgaggcgc acacctgctt agcgtcctcc acacaggagg
     5461 cgtacatgat ctcgtcgccg agaccctgct cgccgtagaa cacaacgtgc tggcccggcg
     5521 taccgtccca catgggttct tcaaggtact ggatgtgtcg gcggaacttc ccaccaaccg
     5581 atgcgccgta cagctcccaa cccttgtccc actcaccgag cgagagatag gtcatcccaa
     5641 gcgtgttctt cgccgccctg ctctcagggt ccagcttcag tgctttctcg caccacttct
     5701 tggcttcctt gaactcgcgt tcagagaagt acgtcatccc gaggttcgcg gagaacatcg
     5761 aggactcttt gttgcggttg taggcttgga agaaggcctc tctagccttg atctgcatgc
     5821 ccatcccggc gtagcacatg ccgaggttgt tccacggctc ggccctctcc gggcgaatct
     5881 cgcagatgcg cttgtagacg ttagccgcca tgccgaagcg ctcggcacgc gaataggttt
     5941 cagcgatgac gaacagcgat agcgcgtcgt ctggattctt gtccagattg gcagcagcct
     6001 ggcgggccgc ttcatccggg tcttcgtcaa tgagctttga aaggcgatcc cactcgctca
     6061 aaactttccc ctcgtggtga ggcaatggcc gtacttctct ttgttgcggc ggataaacgc
     6121 gaaagtttcc ttcgcatgag acatatacgg gtcaaagccg tcttcctgaa tcatcttcat
     6181 ggcgatgaca ggaggaatat gaaagcaatg ccaccagttc ttcttgatgc caatgctagt
     6241 gtaatcgtcc gacttgcgga ggtcgttggt gtagtcaaga ctgggttcta cgtccccgtc
     6301 gtacttgatg accaacttgt catcctcggt cccgatggtg gttttgatgc cggtcagcgg
     6361 gtcaaatgag gattcaaaga ttctcattga aaaagggccg aggttgttag ccccggcccg
     6421 aagtggcaac tacaacaatt actcgctgtt ggcgcgcatt caccggtcgt cccagtgata
     6481 cgccgccttt ggtagtgcat tgcgcagagc cccttggcgt aattggcccg cccgcatcct
     6541 tcaacactgc atgccgtcat agaaatccac ccgttgttta ggcggatgga ttccatcacg
     6601 tcatgttctc agtgtatcag gaacaatctt gcaccttcgc ggagctgtcc gggttgcggc
     6661 cgatcagcgt ccactcgcac agcagttcgc cgcgcgtggc gtcgccggtc ttggcgcgct
     6721 cctcgaactt gatcggacgc agccagcccg tggcccagta gcccggatca aggaacagga
     6781 ccgtgcgggt ccgcatgtag cggttcagca tgatcttgtg ctcgccgaag tccgagatgt
     6841 agccatccac gccgccgatg accacagcct gcgaggtgcg gccagcgttc tggttgacgc
     6901 tcacgccagc gtagcgagca gcacccgtga aggtggcaat cgtgcgcttt tgcgaggagt
     6961 tgcacatcac ggtcgtcggg tcgccgccgt cctgccaagc agcgtccaga gcttgaacga
     7021 acgtggtttc cgtcagagcc gcagtcgtgc cgtcaaccgg cgcggtccac gtaccagacg
     7081 aataaccagc ggtcgtgccg gtcgtgttgg tcgagccagc gaggatgcgg ttacccgcga
     7141 tcatcgactc aagcgaggcg ctcgaacgaa caaccgaggt cgaaccagcg gacgaacctt
     7201 ggttctggac gattgcgtat tcgatgtcac gcttcagttc gcggcccttc ttggcgatct
     7261 ggtacgcgaa ttcttccttg cgaccgtact tgcgaaccgc atcagcagtg ccagagacca
     7321 tgacggtctt cttggatatc tgcgtgtagt tcgacagcat cgtggtcggc gaggcggtcg
     7381 cgtaggtgct gtcgtcgcct tcaacgaagc ggttcgtggt agcggaagcc aggctgtccg
     7441 tctgccactg atggagagtg ttggtgacct tctcgcgggc gaacatgctc agacacgggg
     7501 tttccgtggg cgagatgttc cagatcacgt cttcgacatc ctctgcaaga ccaatcaggt
     7561 cataggtgtc ggtggttccg gctacttgtg ccatttcatc ggttcctttt cacttgcgac
     7621 cgcatcaata gcgccgctgc ggcgtcttga atcgatccgg tctgctgcac ccgcttcatg
     7681 gcctgctcgg ccttcatcac gggtgcggtt gtctttcccg aagcgccagg cttcagggtc
     7741 tttggggttt ggacaactcg cttgtccacg gtggacttgt gctcccgcat ctgccggcca
     7801 taggcggcat cggccaggac ttccaccatc catgcttcag tgatccctgc gaccacctca
     7861 ggggtagcgc cacggctgat tgcgtactcg gtcaggctct tggccatttc cggcccccaa
     7921 cccttcacgc gcgtcttgag ggtttcgttg gcgtgggcga tctttgcctg cacagccttc
     7981 gtttcctctg cgcgcatctc ttcgtcgctc ttgcccaatt gggcctcgat gcggcgctga
     8041 gcggcttgga gctggttgta ttccgcgttc agtcgggtga gcgtgtcgct atccgttgac
     8101 tggatatcga tgctctctag ctgctgcaac cgcttggcga ccatcttgtg gtctgcgaga
     8161 agatcggcgt gcgcatgcgc gagcttctgc aattgcttga cagcttctag ctgtgtctct
     8221 gcggacttcc gaagttcggc ggcctcttgg aacttgcgcg tcgcaccggc ttcaacctct
     8281 ttggcctttg cctgaatgcg ctgcgccgta tccttgaatt gcgcggggac tttgaacttt
     8341 tccccggcaa cgtcgatttc ggcttcatcc tcctgcccag gttcctcact cgactcttcg
     8401 ctcgcctcag catcgacctg ttcggtctgc tcggcttgcg cttcctcttg cggctcttcc
     8461 tgtgcaggct cggctttcgg ttcgtcagtg acgccccagc ggccgagaat ttgggccacg
     8521 gcttgttctt ccgtgatccc gctgctgact tccgtttccg gcgtggtctg atccatgtgt
     8581 gcgttcgttg cgccaatgaa gaaggccccc gtaggggcct agaaggcgtg gcgcgttacg
     8641 ccttgttcag tagcttttgg ggcggtactg ctttggctct aactgagcct ttgcaagtac
     8701 accgtccttc acggctttct ctaagaaggt cttgaacttc ccgaggtagt aatgcatcgc
     8761 tttgacctgc tgcattccgt caatgtccgt tggggacagc ccttggaaca ggcggaagca
     8821 ctcagcctcg atgtgggcaa acgcctcagt cattaaaggc tcatccagca tggccttggc
     8881 tcgcgcgccg cgacggatcg cttcttcggt cttgtcggtc atagcaggag catcgctgcg
     8941 ctttcttcgt cgtcttcgtc gaagggtggc gcaaggaact gctctagtcc tttcagggtg
     9001 gtctccagtg acgcccattg cgccttggaa accatgtgtg gcttcttgcc cttgtacgag
     9061 accttgatgt cggctctctg tacgtcgatc atgtcccgcg ccagcaggta tgcaagctcc
     9121 tcgttcgtga ggttgcggta ccgcttgcca cggatgatgt agttccgccc gcccgctctt
     9181 tggctcgccg atcccttgga gacgaggatg gtgacggttg accccgagag agcgaacgag
     9241 ccgcctgtcg ggttctgctc gatgatcgtg ccggccggct gcggagagaa cgtctccgag
     9301 acgacgacaa cgaatccttc ggcctctagt gtggttgtgc cgtcctcttg ggtgaggccg
     9361 acgacattcg gaacctcgac aggagtctca gccgttccgg ccaggatttg ccaggcattc
     9421 ggctggaatg catctgactg gaacgctgta gacatttcac tttcgagcgg tcagcgtgac
     9481 gatcaaatct ctccacgcat tggtgtagtt gttcatcatc atggactttg tctcagggtt
     9541 ccgcccttcg atggactgat cccaagaccc cgccacgaca tgatcgaagt ggcacgtata
     9601 gccaacgtgc ggcgcgttgt tggcccgcca ttccttgtgc agatacagcg gataccactg
     9661 gctcatcggg ggccattggt gcgtcggatc accgtaggcg caagcgtgag accagttcgg
     9721 cgtgacgatc tgggccgtag ccccgtcctt cataacgcgc cagagttcgt tgaagaaggc
     9781 gatccgctct tgtccggtga ggtgctccac gaaatgagag gaacgaacct ccgacacgga
     9841 gttgtcagcg aaaggaagtc ctttgcgcac atcgtggacg tgcttttggc cgaagtcgat
     9901 ggagtcgatg ccttcccagc cgtccatctt tgttttgccg cagccgacgt ccaggcggag
     9961 tggttcgcta ggaacaggct ccaccgctgt agcaggtagc tcaaccgcgc gcaaaacctg
    10021 acccttagcc actcagttct cctcatgtag ataggacgac acagcatctt tgtagcgctc
    10081 aaaccagcct aggcgaaggt tgcatgtagt acacaaaagc ttcctcacct tgcctgtctt
    10141 gtggcaatga tctattgcca aagacgcagt tttgttgtgc cgccctgcct tggctcttgg
    10201 ctcttgcaaa cagatggcac acttgccgcc ctgatccgca agcatttgat cgtattgctc
    10261 tgtggtcaac ccaaagttgc gtttgagttg atacgcgcgc atttcagcca gcctttcttg
    10321 gcgctgcaat ggcgtttcac tttctaggtg cttgttccac cccttgtgct tattggcctc
    10381 gtagtaccgt ttgtgatatg cgaggtagtc ttctctgctg gcaaatggca tttggtcacc
    10441 acatggtgtc aggagggcca aattttcctt caagatcata gtggcccaca agcacagagc
    10501 aatctatggc acagcgataa ccaagggggc gggcccggtt ccagaagtag agatcctgcg
    10561 tagagatacc gccttctgtt tgggtcttga accaaggctt aggaagctct gggtcacgaa
    10621 agaggtccat ccgccacaaa ttgaacccca tgccagtacc gcagcactcc acaagctcgt
    10681 tgtctcgcgg aatctgcgga cggaagttat tcacagggtc tcgcggatcg ccccagattt
    10741 gagcgacacc acccggcccc ttggtgaagt acaggccgcc gatgcagtca tactcaggat
    10801 gcgcttccat cttggctagc agcttaacca gcccgtcagg aggaggcgcg ttgtcgtgct
    10861 cgatggtcag caggtacttg aacttgctga gttgtggatg ctggagaatc cactcaatcg
    10921 actgagagaa agcctcgcca acctccatat tcagcgccgc aactgtcggc atccgcacga
    10981 aaccgttgtt cggcggcgcg tagaggttca tccatgagtg gactatcttc gccgggatct
    11041 gctgccccgc tgggaacagc atgatcgtgt cgagcttctt ccatgacgaa tcctcacgga
    11101 gacggactgc cgtcttttct aggtcttcgt tgtgcttccc gtactcagga acgacgattt
    11161 gaggcttcat tcatctccct atgcaacaag ccacgcagtc acattcatcg gcccggctgc
    11221 tgccgcatcc ttgaacgtgg cgacgtaaac ctgtccagcc ggcgtgcctg tacaagtgaa
    11281 agagctattg acggacccag cgaccgaaag agacttgtac gctacgcaag aattccaata
    11341 gtcctcgctg tatagcaacg atgtgaaccc attccctgca acgtgcgccg tgcccgtgga
    11401 ctcggacacg aagtagccaa ttgcggtctc cgcgtcttgc gtagtcgata cgctcggaga
    11461 atcgtacgga gtggatgtgt cggcaacctg atcgcttaca tcataggaag ccgatgcagc
    11521 cccggcaatc tccacaaccg caagatgccc gcctcctgtc agcgtcattg tgaatgtgtg
    11581 gccgctgccc cccgggttgt tttcgagccg tgcgattctt gagccaatgc tggtgccgaa
    11641 gttatcaaac ccggctccga tggttgtgat gcttccggcc ttgttgtgcg aaaaaacagc
    11701 ccccgccccg gcatcatggt ttgccacaag cacaatggtg ctcccggccg ttgacgtgac
    11761 cggccccacc gtcactgtcc cggaattgac ggcgtgccca agaagtgttg ctgtggccat
    11821 cagccgttta ccgtgttaaa cgccagacgg ctcatatcaa tacaccgtgg tcagatcggg
    11881 aatggccgcc ccagcagcct cccaaacccg acgggaaagg gtcatttcgt cgccattgat
    11941 tgacgtgccg gtgaaggact ggcgagagct ggtaggactt gccgggaaat gccctgttgg
    12001 ctttgtaatc tgcgtatcca tccatgcctt gatgcgcgcc tcgtaaggcc agggccacag
    12061 cgggtcagtc gtcaccgtgt caaagttagt ctcaccccag tggctcagaa acgccccatg
    12121 gcagtaaaga atctccgccc cagcggaacc agcgcgctgc acgatgtttt gtggggaggt
    12181 aggcgagtcg gtgaagcggt tgttgctgtt cgtcgtcccg cttgtgtcag aaccaagtat
    12241 ccacccgcca aagcagttgg tgaccgtcat agatccagct gacaccgagt acccgttcgg
    12301 cgtctgcccc gcttgcgtgt tgttgtcgtt gtcccagata gagcagttct ccgcgctatg
    12361 cgtcgggctt ccgatgttgt cggttctgaa gctcgataga tcgcagccgg cattcaggac
    12421 aatgcatcct ttgtggcgaa tgtcacgctg gtgtttcggg tggtagaagc tgccccactt
    12481 gaagccaaag ctcgcctgat tctccacagc gggcccatcg atgtccacgc agttggccca
    12541 cagagtttgc tttgaatcca cccaagagcc gttatcagat ccatagtgcg caaacgtggc
    12601 gacaggctga tcgcagaacg cccaatcaag ccttgcaacc acgcggcgaa acacgtttgt
    12661 cccagccgtt gcgtctgccg agtttgaccc ggtgttgcac atgtagcggc cggctccgta
    12721 cagggcgcag tcctgcatca gacagccagt gcccaggtaa aacgtggcgc cgtaagtgcc
    12781 agactggcgg cgcctgaaca tgcagcgggt aatgcgggac gtatccgcgc cgaagttgaa
    12841 gatcgcctcg tcgtcgtgct gggtatcgtg gatgaagtcg aacccatcca cccagatacg
    12901 agagccagca gtcaatcgca ggatgctccc gtagtagctt tggctcgact ggctcagcgt
    12961 cacggaaccc ggattgatcg cccggatcac cgtgaagttg gcccccgtcc cactcggggg
    13021 cgcgttcccg gtcgtgtcgt tcaaccaccc gctcgtgccg atggtgtaag tcccatcagc
    13081 cacaaggatg agcgcatttg cagacacctg agatacggca tagtccaccg aggcataagc
    13141 cgcgccaagg ctgttgccgt tgttcgcatt gctccctgta gtgctgacgt acttggttga
    13201 ggagtaggtc agaccagaga gcgcagttga atagagcccg tcgaaatacg catcttgggc
    13261 cgctgaatcg taggcgctac cagagccgcc agacgcgtta ctaagccacg gaaggtagta
    13321 cgccattacc agacggagta attgcgcatg tcgagccaga gcttcgccgc ttgtgcccgc
    13381 gagccggtct gctggatctc gctaatcgcg atgctagccc gcgttgcccc ggtgctcaga
    13441 attccaaggc cgacgctcac tccacgagtc gctgccgtag cttgtcccca cgggtcggcc
    13501 ccgagcatct gcgtcaggtt cgcagctccg attcccatcg taaaggcatg agtcaggttg
    13561 ctcgtgccgg acgtgtttgc cgccgtggtt gcagaggaca catgaacagc ccaccagtat
    13621 tcgcccgggg caaggcgcgt ggtgttgttg aagttcattg tcaacgcacg cgcgccgaag
    13681 acactagccg tggcgttgga agaccaagtg cctgtgtagg tgttcgaacc ggacaggatg
    13741 gatgacaacg aagagccgtt cagcgagtac agaaccccgc tgatactgat cgcagcgccg
    13801 ttcgtgctgt tgttggtcgt cgtctgcccc gacatcgaga tgaggcaacg catgttgctc
    13861 atgtccagtt ggtgctgaat catgaaggga acgatgctga cgcttgaccg atccgcagct
    13921 aggccagtcg tcgtcacggc gttctcgttg acgtaccggc tactcggcac ctgagaaata
    13981 ctgatcgtgc ttccgttggt cgagatactg ataccgccaa cacccacgag gctagacgtt
    14041 gccggggcgc tgatgaccac ttgagaggcg ttcgtgcccg aaagcgtcag gttcaggccc
    14101 gagaggccga tggtcgagcc actggctgtc gtgttgccag cggtattggc tcctagcagg
    14161 ttgatgttgt tgttctcagc cgcagccccg ggagcggcaa cggacgcgga caggttcagc
    14221 ccgttgctgt tcagcgtcat ggaggcgctg atgttcgccc ctgcgaaggt cgtccctgtt
    14281 ccagcaaact gcgtgctgtt gtagctcgca gtcattgtcc cgttgttgga caggccaaac
    14341 gtgatgccgt tgctattggc gaagttgacc gttcccgtgg attggctgtt cgcgccggca
    14401 gagatagccg cgccgccacc agcgccacca gtagccccag agatgacaat ggagccgttc
    14461 gagatgccaa ccgatgcccc accagtgcct tggaggatca acccgttaag gttgaccgtc
    14521 ccgctgctgg cctgagtggt gttgcccgtg gcgctgaccg tgacagtccc gacaccgaga
    14581 ctgatagtgg agccgttgga cgaaaccgac agcgccccgg ttgccgagag gctggtaaac
    14641 gccgggccag agacgacaat cgagccgttc gacacaccgg cagacacgcc gccagtgccc
    14701 tgcacgatga tggagctaag tgcaatcgtg ccgctgctgc tctgagtcgt gttgccagtg
    14761 gcgtagaacg tgccggtatt gacaccaccc ccgccgttcg ccgcgctcag gtcaagacga
    14821 aggccgttgg agttcagcgt catcgacgcc gaggcgttcg tgccgttgaa cgtggttccc
    14881 gtgcccgcgt atccggcagc gttcaggctc aggccagagc tgttgaccgt ccacgtgacg
    14941 ttcgtctgag cggtgttcag gccaatcgcg tcattcgatg cgcgagccgt ggtcaggccg
    15001 ttgtgggaag ccgtgacctg agtcgagcca gacatcccga aggtgatgcc gttgctgttc
    15061 tcaaacttta tcgtgccaag actcgtgccg gtctgagtac cagccgcgat gacgttccca
    15121 ccatccccgg cgccaccgcc ggacgggacg gagatgacta ccgatccgtt cgataggccg
    15181 accgatgcaa cgccagcacc ttggatctgg agagcgttaa ggttgacggt tccgctgctg
    15241 ttctgggtgg tgttggagac ggcgctaacc gtaacggtgc caacacccag gctgatggtc
    15301 gatccgttgg agcttgcgga cagggctcca gtggcggcaa gactcgtgag atcagggcct
    15361 gaaacgacga tggagccatt ggagatgccc gcagagatac cgccagcccc acggacgatc
    15421 atggagctga gcgccatggt gcccgtgctg ctttgggtcg tgttgcccgt agcgtacaga
    15481 gtcgccgtag ccgctccacc agccgcagca gcgctcaggc tcagcgtcag cccagccgag
    15541 ttgcttgcag tcgtcccgct cagattcgtg agcgcaagcg tcgggttgcc gtggctatgg
    15601 ttggacgaag cgtagtccgt cttgaccgtg ccgaacaggc cttgagtgcc ggtcgagaag
    15661 ctaaccccgt tggagttcgc gaaggtaagc gtctggaacg cagcagagcc gttgccggcc
    15721 gaatacgctt gattgctctg cgaggtcagg ccgttgtggg aggcagtgac cgtggagacg
    15781 ttggtgccga acgtcagccc gttggagttg gacaggacaa aggccgtctg aacgggggca
    15841 gagaacacca gcgtatcggc attcccgctc agagtgacag cgccagcacc ctcgaagacg
    15901 atgttcgtcc cggacagcgt ggacgcacca acggtgttcc cgccgatggt catgaactgg
    15961 ttgtgagccg agttccagtc aagcgggcgc accaagtccg tcgcagccgc agtggtggtc
    16021 actccagcgg agttgaacac cgtaacggtg cccgtcatgt tggcgacggg gttggacttg
    16081 acgtgagaaa cggccattac tgcgcgattt cagagtgcga cggacggcca tcggggccac
    16141 ggacaagcac gcgcttggcc ttgaccgcct gctcgatgct cttttgtcct tctgcgactt
    16201 gcttttgcat gtcggtgagt tgcttcaatg ctccagctac ctgatccagc gccttctgca
    16261 tgctctggat ctgcgtgccg gtctgcttgt cgggcttctt ggccatgtct tggagttctg
    16321 cctggctctg acggtctgcc tgcttctggg cagcgtcatg gccgatcttc agttgctcgc
    16381 tctggctctg cgtctgagcg ttcaggccag cgatggtcaa cttggtgttg ttgtcctgat
    16441 cgttggtgta cttgccaaga tcggcctgaa gttgggcctt ccactcttcc aatgcgaggc
    16501 gctgctgctc aagttgtgcg tcgaactgag ccttgaggcg ctcacgctcg gcgtcgcgca
    16561 tgtcgttgga ctcttggagt tgcagttgag cctgaagctt ctgcatctcg atctgagcct
    16621 gctgacgagc caactcaagc tgcacttggg ccttgccctg ctcgatcatc atctgagcct
    16681 gagccttggc ctgttccttc agcacctcag gcggcgggcc aggatcaggc atcggctcaa
    16741 cctgtggatc agcccagaac tcgttgtggt ctttgtagcc agccgcttgg gtcaggcgct
    16801 tgagtgcgtt gtaaatcttg ctcgggttcg ccaacccaac ctgcatcgca agctgctgaa
    16861 tctggagaac ttgcgtcagg aaggcgatct gttgcggctt gtccccagca cccaggccaa
    16921 cagcaatgct catgtccgca cgcttcaccc actggcgggg atcgactaca acccattggt
    16981 tgcggaggcg gacaacctcg gccttgcggg cgtgcttgag ggtcagggcg tgtacgactt
    17041 ggaacagcgc cttgacgcca gtctcagcga agatgcgggc gatgaacttg atgcgctgct
    17101 gtgccgcgct cataagcatc tgagccccgg tggcggtctt gttgagcgtg ttcgggtcga
    17161 gaccttgggc ctgctcgttc acacccgtgc gcttctggct gatccggtcc acgtactcca
    17221 tcaggggcac ggagtccatg gccggattgc cttggatcag cggtgcgata gcgttctgca
    17281 ccgggccatc cacacgcacc acgccacccg gacgactcac gagcatgtcg tcaaggttca
    17341 cctgattcac gtcgatggcg aaccggccgt tgttgctcag gtactggttg tccagcgttc
    17401 cacgaaggag cgcggtcttg atgtcttgga ggtctgtaac cgcgtcagcg acgctcagtc
    17461 caacgtgctg gtgaggcaga ggggtgggac acttggcaac gagggtgacc agatcacact
    17521 cttcgtcttc aaggatggtc ttcccaacga cgaccacacg acgcaactcg gtacgaccgt
    17581 catcattgga gtcatagcga atccagacat tccgaacccg aagcctgcgg gcagcggggg
    17641 aagactcttc gccctcgttg tttcggaacg ggttgaagtc gtcacggagc gattcctccc
    17701 attgctgcgt gctgtcggcg tcgtcttgaa ggtcgtcgtc aacctcgaat ccttcatccc
    17761 gaagatcgga gatggtcttg tattcccagt attcgaggaa gtcgaggcgc gggtcttgca
    17821 ggttaagacc acgagcgttg tgagacacga gcacgcgctc aggcggcacg ttctcgatct
    17881 tgacgcatcc gtactctttg acgcgctcta gctcaacctc gaagccttct tcttcctgct
    17941 ccagcttgac gatctggacg gacgggtctt gagagagcag agccacctca tcctcggtca
    18001 gcccttcgta cttctccttg gtccggtcct cgcgctcatc ccagtaagcg atgacgtagc
    18061 cggtcttctg gagcagggca tcgtgcgccc agctatacca gacggtgaac cagtcgttct
    18121 tctcagtgat gacccaattg acgaactcgg tctcttgctc agcggcggct acatcctccg
    18181 ggccggtggg attgaacatc accacctgat cgccgccgca gaagatgtcc gaaagctgcg
    18241 gcttgatcca ctcgaccgtg tcccacacgt cacgagagat gacctgagag cggccctgca
    18301 cctcgttgcc catcgggttg ccgaggtagt agtccagcgc cttggtgcgt tcctcggaca
    18361 gattgccgta gctatcggcc agcgtctcgt gacgctcaat cgctgccaac aggctttcgg
    18421 tcttcattct tgggtcttcc gggtccgcgt ttctgctcta gtgcaatcac tcggtctgtc
    18481 agcttgtcaa tcagtgcggc ttggcgaagc agtgagctgc tgctcatttc aagcaggcgc
    18541 ttcaggtctt cgatgtcctt gaccatctga gcgtgctgaa tcgctcgcgt gattgcgctc
    18601 atacgatccc catgtctgga tacttgatcg gggcgaactt cttgctgttc ggctcttcgt
    18661 aggcaaccgc catcaggcca aatgcgtccg ctccgtggct ggcccagtcg tgctcagggc
    18721 cgaggccgac gtttcgctcc gggtctttct tttcgtgata ccagccaaga gcatcccggc
    18781 cagcactggt ggtgtcttcg ttgaaccaca tggacgggaa caggcgccgc cctgcctcga
    18841 tgcgggcctt tgctgcgcct ttgccttggt tcgggaccac tgtgaccttg tagccagcct
    18901 gcttgagcgc tgactcgtag ctgacggcat acaccttgtc ttgagtggcg ccgtcatggg
    18961 gcagccagaa ctgtgccctg tccttggtgt agccacgctc acggcaccag gccaagtgag
    19021 cgtctagcgg ctgtcctacg gcttcgtagt agtccagcac gcggacttct ctgcctacga
    19081 actgagacac ccacatggcg aaggcatcag cctttgcacc cgtaccgcca atgtcgcaga
    19141 acagacgata ggtcatgagc gggtctggag acaccctgcc gatccggccg tctgccttgg
    19201 ctttgttcag gctcgcggcg tagtaagcgc catccacgat gctctggaag tcgccttccc
    19261 agatgtgggc gtactgatcg ggcctggcct tgaaatcgcg ctgtctctgt cgctccagct
    19321 tgtcggggaa ggctgggttg tccttccagt tcagttccac gcacttgacc atcgggtctg
    19381 tgctctgcct gaaccggctt tcaaccgctg cgtgcttgtt gcgcgggttc cacgttaccc
    19441 acagttccgc gttccagtcg ccgccctctt cacgcagcgt aggctctacg atgtcccatg
    19501 cgtcagcggt gacgggctcg gcctcgtcta cccacagcag gagaatccgc cccttggact
    19561 tgatggacat gatgttccga tcaaggcccg agaagctgaa cgcgatccgc ccatccttgc
    19621 ttttgatgta gcggtcgcct atctcgtagt agctggcaag gaacggctct tcctcgatgg
    19681 cgcgtttgca ctcttcaagg gatgagtcgt ccaggctgtt catgaactgg cgagcgcaga
    19741 ggatgattcc cctgtctcca gccatgccgt ggatgtaccc acgtaccgca gccatctttg
    19801 cgaatgagcg agtcttaccc gacccacgcc caccgtatgc gcctcttacg tctgcctttc
    19861 cttcaaagac agggatcagc ttcgggggaa gctggatttg gactgtggtc acttcagagc
    19921 ggttagctcg atgcgatgca cagtctggag cgggttctcg ctatcccccg agacggtcac
    19981 ggcactcagg tccgcaacgc tcttgcgcaa cagaatctcg atggctttca tccgcgtcat
    20041 cgacaagtca ccatcgcccg taagtgcatg tttttgcagc acatttatga gctgagtggc
    20101 ctgaatcttc ttgcggatgt cttcctgatg gagtttgttg agccgtgcgg ccattggcga
    20161 ctccccttac gggtgtggtc tgtggataac tgcccgagag cgcaacgtct accccgccgt
    20221 ctagatcggc gaacgctcta cgtgttgtgg gttcgggctg aggggtggcc cagcgatcgc
    20281 tgatttcgtg ggaggagacg aaaggagggt gtcagcgacg ccgggccgaa actgctgcat
    20341 ccatcccgga gcagcgttcc gctgactggc acctagccct ccgagtggat ggcctcgatc
    20401 ttatgccttc gcgcatcggg ttgcgcggag actaacacat tcaattccga attggaagat
    20461 caaagtgcaa ctttttcttc tccgaccaat acgcgcttca ccgcttccaa gtcgacctgt
    20521 tcaaccttcg gtttcggcat taaacgcccc tgttgcacaa catctgccgt cctgcatcaa
    20581 tcagatgctt catcccttcc agcgactctc ccagcgcctt tgctgccttc ctggggtgcg
    20641 atctgctcat gtagttccac gagattgcaa tgcggtgctt ctctggcaga gccgaaacac
    20701 ctttctgcac acgctgggca tctagttgat caactgctgg cgctttgttt gccgagtctt
    20761 cccattgctc tgtactccga tacatgcgga acatcggggc gcagggagca aagccgagtc
    20821 cgtcgcgccc attggcccag atgctccagt ttcggagtcg tgcatccatg gtctggtgct
    20881 ttgggtccac cgcgttgaag tccacgtatc tgctcaagcc ttagccctcc actccagccg
    20941 cgccatgaca acctcgtcat ccgcctggct gtatctgtcg cacacccatc cgaatggatg
    21001 gaacgttgcc cgccacttgc ttagcccgca gttcacatag ccttgctttg ccattttccg
    21061 aaggctcctg tctcgctttg ggtctttggc gtcacgcaga aggccatggc agcaggtgat
    21121 gcattcacga ctgcaagcca cggatgattt ccgccaacac ctcaccgaag ctattctcgt
    21181 ctccgtggat ggctactcct agcatgtagt tccagccgct caggtctacg gtgataactc
    21241 ggcggctacc gtgtaacgtg gtcttgaagt agcccatgcc tctgtgttct tcccatgagc
    21301 tgtcttttgc ttctgggcag tagtgctgga tggcgtggac tacttcggag gcgttcacac
    21361 agattcctcc agagcctgta tcgcctcctc aggcgtcgat acaagccaca ccttgccacc
    21421 gcaccaccgg ctgatccact cgcgttgatg ctcggtgagc ttcttctcaa tggagccgcc
    21481 gggccgtttc acctcgatca gcacggtttt cccggcgtat ccgcacacca aatccgggaa
    21541 ccctccaccc atgcgggaag agtcttgaac gtaggccccg tgcgccctga gtgcttgcac
    21601 gatttccccg tgattggcgt ctacgcgggc tgcgcgcctc accaccaccc cagcgcagta
    21661 ccgaagacga aagcaaagat gccgcagcac accgcgtaga cgatccagct tcccattgcc
    21721 tccacgttgt ggaggtcctc ttccagcgac cctggctctg tcgggatgta gtcaacgcct
    21781 aggccggctt cttgcatttc gtcgcgcatg gttggctccc gtctataagg ggatcgaatc
    21841 gtcttcgtcg cgtgaacgtc tttgctgctt ttgcggcgcc gattgctgct tctgctgctt
    21901 cggcttgaag ctgaagctca tccagcgccg cccgctctcg gcggtcttca gccatgcgtc
    21961 catgaaatag gcctgcccgt caatgagcgc atcgccggaa tagtccgggt gcttgtcgct
    22021 cttcttgtct tcgttcttga agagccgccc gctgttgtct cgcgtctcgt aagccatcat
    22081 tcctccttgc cgatgagttc aagcaacttt ggcccggctc gccatagtcg cggatgcgtc
    22141 ttgatggtcg gcttcaagta gccgtctcgt gtcaggcact tgagcgcttc ataagctgtg
    22201 cgtcggtcgc tgaacccgaa cttgatggtg acatctggtg cagacatctc ctcgtcagga
    22261 ttggcagcga agaacgccac gatgggaacc tgcgcagagt gcttgatgcc gcgccctacg
    22321 ctcatttgcg cttctcctcg catgccttac acgaaaacag gttgcgcaga ggcccacgca
    22381 tcttcccgcc gctgcgctct ttgcgctggt ggaaaaagaa gcaaagcggc cagataggaa
    22441 gaccttctcc tgcgtagcgg aaagcggtgt ccttggggcg tttgatgtcg tcgctgctca
    22501 tgccacgcgc ctcagtttct tagccagttc cttcaactca tgaggtggcg cggtgaactc
    22561 tcgcttgtcg tgctcctgaa gccactcgtt ctttcccgct gtcggagcgc gctcgatgta
    22621 cttctccttt gcaggccaca ctgatcgcca gcccatgagc gttgactgat ccagcgccgc
    22681 gttagggcag tgtcctgcgt ccttgatgcg ctgaagctcc accacgatca tctgagccgc
    22741 cctgtcagtg aatggcacct tgcgcatgct cctgcgcatc tcgacgaagc cggcccatgc
    22801 ctcgggatct atccatgtcg gaaggctcag cggacccatg aatcactccc ccatgtccgg
    22861 ccaaaacgaa cgtctcggat agttgacggt ttcaccccgt agcgctgcgc cagaacccgc
    22921 gatggctcat cgctaaggcg gatggcttga acgtgttcgt gcagcttggc ctgtcccgac
    22981 ttgataacgg cgcggcgcat cgactcgcta tctacgatcc ggcgccctgc cttgatggtc
    23041 ttgcggttga tctccccgta ggtcatcgcc cgcaggcatt tcggagagat gcacagcggg
    23101 ttgcagcact tggtggtgac gcgcttgttt ttgtggacag gctttccgag aagagtctcg
    23161 aagacgtagc gccgcacctt gacgcacttg cagtcaacac gagcaatcgg gttgccgtcg
    23221 ccgttgcagc cgaggttcca cagcaggcag tcgccctcct ctgtggcgta gtggtgcagg
    23281 ttatcgagcg ttagcttcat gcttcggctt tacctttgct tgaccagtca acatcgcggc
    23341 cagtcgttcg gcgtggcact tggcgcatcg atactggcgg ccaattccta caccgccagt
    23401 ctggtaatgc ttcaggcact taccgcagaa catcagcagc ctcgtacata gttgcgtcct
    23461 cgccacactt gccgccgtct ctggcctcga tgcagtacgc gaatcgtcct cgtccacgca
    23521 caggagaagc ggcgcagcgc cagatcgtgc caggtttgtc ctgccttgcg tcgttttgga
    23581 ctaggcggtg tttgcatgcc tggcacttct cgcgcatgga ctcccaatcg gtgtaattca
    23641 tgcgacgatg gtaggcgcgt cgcgcgattt gtctactagt gcaaaccctt agtgcttgca
    23701 tctggccgac acttggccta tgatgatctt gtcaggtgtg gcagcccgac attggttacg
    23761 cgcgaacccc gcgagtgttt aggtggggcg tgtgtgagtc gaagtgcgct cgtaacccgt
    23821 gccgagactt gcccacgcca agggccacgc cccacctaag tgctccgggg ttttgctttt
    23881 ggcgtcctga catccggggc gaccattgag cgaatcacac cgtatctaca tcccgatgat
    23941 tggtcgggac tactgcgtga ccctcagcga gcagcaatgg ctcgcccgta ggcatgggct
    24001 gactctggag caggtgcgct cccttgtgcg tttggtcgat ctgtcggggt gcaagaccgc
    24061 gactaaggcc cagcgggttc ttgagtcagt cttggccgac agcagcaatg ccacggcgtc
    24121 agggcgcgtt agctaacagg ccgtcaaggg gccgtacccg gattcatccc agtccagagg
    24181 gaatgtctgg tcgctttcca cagcctggcg ttgcaggggg ccagcctcga aagagggaac
    24241 ggtgcgtaaa agggtgatct acagggcggc agggaccagc cggacgacct aacccagcgt
    24301 cttaatcggt cagcacccga tattgcgtgt ggaacgcatc agcgaaggca agaaccccgc
    24361 agggatcacc caacccctgc tttggcagag gtatgcctgt acggaacaac acatagggta
    24421 agtacctata tgcaaacctc accacagcgc cgatgattca ctcatgcgct ccacggtacg
    24481 gggcgc
//
LOCUS       DAOQEW010000036        24415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_490_length_24415_cov_8.244828, whole genome shotgun sequence.
ACCESSION   DAOQEW010000036 DAOQEW010000000
VERSION     DAOQEW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 24415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24415
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_490_length_24415_cov_8.244828"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..513
                     /locus_tag="PLS35_06020"
     CDS             16..513
                     /locus_tag="PLS35_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNI18247.1"
                     /translation="MADQSSFDVVSEVNMQEMKNVVDQATKEIKQRFDFKDSKTELTL
                     KEKEKELVVLSDDEYKLNAVIEIIKSKCVKRGVSLKAFEYGKIEEALGATVRQVIKIQ
                     SGISSEKAKEITKAVKESKLKVQAQIQGEQVRVISKSKDDLQSAIAFLKGKDFGIDLQ
                     FTNYR"
     gene            660..1682
                     /locus_tag="PLS35_06025"
     CDS             660..1682
                     /locus_tag="PLS35_06025"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18248.1"
                     /translation="MTKFLASMFLLAGLLLGGCGGSDPVVVIQLHPTNPDILYIATND
                     YIYKSRDGGKTWSNLSKGMSYSRVIAMAVDPVYPATIYAGTKGDAVYKSYDGGQRWTS
                     VRTGLDDATISSVVNQFVFDPSDDNHIFLATTMGVFESKNAGERWVKRMEGMKEVLMV
                     VTLGMDPAHPSVMYAGTSGGVYKTNDQGAHWLQVNNGLVPEGMVKTSRALGVTSVQVD
                     PFAPDTVYAATLAGLYKTTDGAVSWVRIGESLQDQMIVAMVLDHAQAGVMYLAGRDGI
                     HRSGDGGKTWIVLNEGLTTTNIRSLAQSAMDPHVLFAGTNGSGLYKSVDGGAHWQAMP
                     SVFPKD"
     gene            complement(1723..2817)
                     /locus_tag="PLS35_06030"
     CDS             complement(1723..2817)
                     /locus_tag="PLS35_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18249.1"
                     /translation="MIQTSTLHPRHWLASALIGLLLVGLLPAVQAGEFKSDNPLNQPG
                     KIYWCPNRAADKRITATPDAGCTALYDAERDEKKPAALNQDERNDAATKEPLKILDIQ
                     NEASKFTRDYREFIDCCASEWDSLDRIDELLEQSNHILMSVQQKGIYNSAGFGVGSGA
                     SGLGGGPGQSPKLGTFARQWTLSEIVGTVAIARDDLKKLRTRMKGLITRYEKLDSLDY
                     EQRGREERRIEDEREAITHDLRPNRPATSAPTGMDIQDTTIPTRIGGDIENTHLNRSL
                     NPSFGADIGTTVSPYSNVNESLRPRRGEDLRDSVLTDRVGTQTQDTTLPNASGFEINA
                     AENAEQSSSAHIRGVGPSIGDSSLNSQSRR"
     gene            2953..4245
                     /gene="asnS"
                     /locus_tag="PLS35_06035"
     CDS             2953..4245
                     /gene="asnS"
                     /locus_tag="PLS35_06035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNI18250.1"
                     /translation="MPVIYIDEAATHAGQEVTIRGWLRSRRDKGKLHFLIVRDGTGDI
                     QAVVSKGAVGDEQFAHSAELTQESSVVLTGTLRQDARAPGGYELDVKRLAVLQVAEPF
                     PIQPKEHGTGFLMEHRHLWLRSSRQHAVLRIRHEIIRACRNFFDDRGFTLVDAPIFTP
                     NACEGTTTLFQTQYFDETAYLTQSGQLYSEATAAAFGKVYCFGPTFRAEKSKTRRHLM
                     EFWMVEPEVAFAELPDMMDLAEQFLAHIVAAVLKTRRAELQILERDIAKLERVTAPFP
                     RITYEEAVDILQRKGNPAKRGDDFGADDETILSKEFDRPVIVHRYPAALKAFYMETDP
                     ARPDLALCMDVLAPEGYGEIIGGGQRIHQHEKLLARIREHHLPVEAFQWYLDLRRYGS
                     VPHAGFGMGIERAVAWICGLEHVRETIPFPRMLYRLYP"
     gene            4556..5479
                     /gene="rsmH"
                     /locus_tag="PLS35_06040"
     CDS             4556..5479
                     /gene="rsmH"
                     /locus_tag="PLS35_06040"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNI18251.1"
                     /translation="MDSARGSHEPVLVDEIVFWLQCKPGGVYVDCTLGYAGLATRILH
                     HTAPDGILIGIDRDADALEESRARLQGVMPRVHLRHGNFSQLKTLVAQVGVQHVDGVI
                     FDLGVSSPQLDRPERGFSFRDDGPLDMRMDRSEGRTAADLIREMPERELADVIYQLGE
                     ERYSRRIARAIVEARTQRVIGTTRELAAVVEHAVPASYRHGRIHCATRTFQALRIAVN
                     RELEVIEPALRDAVDLVAPGGRVCAVSFHSLEDRIVKHTFRALANGPDAAVRVLTKKP
                     VIASESERDHNPRSRSAKLRVVERMSKESMQ"
     gene            5476..5808
                     /locus_tag="PLS35_06045"
     CDS             5476..5808
                     /locus_tag="PLS35_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNI18252.1"
                     /translation="MKAVAFAAVTSLVLLFVWERVDIVRIGYHIERLKAQKVLLERER
                     DELQVKLSGLTAPERIARLASDKLGMLQPEKGQVVVINFEPEAPANPVAAEGEIRIAK
                     NMVTRRAR"
     gene            5808..7526
                     /locus_tag="PLS35_06050"
     CDS             5808..7526
                     /locus_tag="PLS35_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNI18253.1"
                     /translation="MASPSFRGRRILVACGLSGAFILVIVRLVNLQVMQSAELTVKAD
                     RQHQKNVTLEGARGTIYDRNSKVLAMNMDVPSVFGVPASLGNPGVAARNLSPILHVKA
                     TELEKKLKQEKHFVWLARKLEPEQGRRLERLGLDGVGVVMEGRRFYPKGPLLSHVLGF
                     AGMDDRGLEGVELRYEQYLRGEKRAVVLQRDALGRAVFPKGLNEEGAAAGHSLTLTVD
                     EVVQYIAEKELDEAVTRSSAKSGTMIVMDPRSGAVLAMAVSPRFDPNTVGALAPDRWR
                     NRALTDTYEPGSTMKAVIAAAALEEKVMTPGSMIYGENGQFAIANTIIHDHEKAGWMT
                     FAQMIQKSSNIGAAKVGMALGEWRVFDYLKEFGFGDKSGIDLPGETAGLLRGPRQWGK
                     RSLASISMGQEVGVTPLQMVTAISAIANGGVLMKPYVVSEIRNGKGQLVAQNMPQAKR
                     RIISADTARTLTTLLEGVVTHGTGTKAAIPGFRVAGKTGTAQKVDPRTGKYSSTLLVG
                     SFLGYVPAEDPRLAMIVVIDEPRGEGWGGVVAAPVFRRVGEQVLNYLGVAVDEPVKLA
                     MAGLES"
     gene            7528..9030
                     /locus_tag="PLS35_06055"
     CDS             7528..9030
                     /locus_tag="PLS35_06055"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNI18254.1"
                     /translation="MTLDELISPIQGRLGVLERSGDQRVAIDALTDDSRHVQSGALFV
                     AVQGERVDGHEFLDRVVKAGAAAVVVGRHVQVGSTPCIRVQDSRAALGMVGSRFYGDP
                     SAALRMVGVTGTNGKTTTTYVVKTMLEAANRQVGLIGTVAYLVGKESIPASHTTPGAL
                     ELQKLFARMVEKRLDTVVMEVSSHALALDRTAGSEFDVAVFTNLTQDHLDFHLDMERY
                     FQAKRKLFAELGQPDSRKTKKRAIINADDPWGARMREACTVPVWTYGLHQQADIHADE
                     VTLSPAGTSFTLRTPAGTCPIQSRLVGEHNVSNLLAAIGVVLHEGLTLEQVRAAVGAV
                     SNVPGRFERVDAGQDFTVVVDYAHTEDALVRLLTAAQALRTGRIITLFGCGGDRDRTK
                     RPKMGRAAVQYSDVVILTSDNPRTEDPASILSEVEVGVKDALTARSHVRYRMIADRRA
                     AIEAAINEAKAGDMVLIAGKGHEDYQIVGTTKHHFDDREVAREMIQARRS"
     gene            9076..10512
                     /gene="murF"
                     /locus_tag="PLS35_06060"
     CDS             9076..10512
                     /gene="murF"
                     /locus_tag="PLS35_06060"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNI18255.1"
                     /translation="MALFTVEEICEVLSAKSPTGLSPQDLKQRIRRVVTDSRLVRKGD
                     LFIAFQGERFDAHTFVPKVFAQGAVCAIVQEDYRLPPMKPRTGAPIVLGVRDTLEAFQ
                     RLATHYRNRFAIPVIAITGSNGKTTTKEMVAHVVAQRWKTLKTEGNLNNRIGVPQTLF
                     QLAPRHQAAVIEMGVDQQGQTTRLCEIARPTVGVITNIGPDHLEFFGSMEGSAQAKAE
                     LLDHLPQDGSVVLNADDEYFDYLAARAQCRVVAFGSSSKAAVRAANVRTDDKGGTLFG
                     LVLPGKTRQTEVRIRTQGQHNVSNALAAAAVGHALGLSGAAIAEGLAKFRPAAMRSQI
                     SSSHGVQVINDCYNANPASMKAAIQLLAQLGRDKRSIAALGDMLELGADTKRMHRDVG
                     AFLAEQGIGHLMACGVLGRELAAGARQAGMPADRITEHPDAQAAATALARMVRQGDVV
                     LVKASRGMRMEQVVDALTGMRRVAKKAC"
     gene            10570..11646
                     /gene="mraY"
                     /locus_tag="PLS35_06065"
     CDS             10570..11646
                     /gene="mraY"
                     /locus_tag="PLS35_06065"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNI18256.1"
                     /translation="MLYNWLYPLHTQFSFLNVFRYQSFRIIYAAVTAFLIAFVMAPWV
                     IRKLQEIKLGQQIRDDGPKRHLAKSGTPTMGGILIIFAVVLSTLLWADMTNRYVWLVV
                     VATVGFGAVGFADDYLKFIKRQSKGLSAAQKFSGQFLVALAIGVFLYTLPSYTTKLSV
                     PFFKFFTPDLGWFYIVFVILVIVGSSNAVNLTDGLDGLAIGPVMIASLAYTIVAYVTG
                     NRVMAEYLLIPYIEGAGEIAIFTGAILGSSLGFLWFNTYPASVFMGDVGSLPLGAALG
                     TVAVISKHELLLLLVGGVFVIEALSVILQVGSYKLRGKRIFNMAPIHHHFEMKGWDEP
                     KVVVRLWIIAILLALLSLSTLKLR"
     gene            11657..13045
                     /gene="murD"
                     /locus_tag="PLS35_06070"
     CDS             11657..13045
                     /gene="murD"
                     /locus_tag="PLS35_06070"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNI18257.1"
                     /translation="MDVKGLQVTVVGLARSGVGAARLLNHLGARVTVADRKEPQELAT
                     ILSQLDQSGIAVKVGAQYESALEGADLVVISPGVPTQLDALNRVRARGVRVIGELELA
                     SRYMTAPIVAVTGTNGKSTTVTLIGKFLKESGKRAFVGGNLGIAASEAALTCVQAKPG
                     SPPPYEYVVLETSSFQLETIEQFHPFIASILNVTLDHMDRYNSVEDYVAAKARIFTNQ
                     TAGDYSLFNLDDTRVAALRGNTEAAVVGFSRSGATVSGVAGAPVLDGDLIVTTVRGQR
                     EEICRRGDMRLIGLHNVENVMASVTYGLLCGCSIEAIRAVLRFFPGLEHALEVVRERR
                     GVRYVNDSKGTNVDAVLKALEGIEQPIWLIAGGRDKGGDFSRLEGAIRERVKGLILIG
                     EAAARIQAAMGDFDRCRPAATLRDAVELAAREAQPGEVVLLSPACASFDMFADYQDRG
                     RQFKALVQALPA"
     gene            13081..14298
                     /gene="ftsW"
                     /locus_tag="PLS35_06075"
     CDS             13081..14298
                     /gene="ftsW"
                     /locus_tag="PLS35_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNI18258.1"
                     /translation="MAQHALGTLMLPWSTTTQRASKRVPVDPALLAVTLILALIGVVM
                     VFSASAVVAGNRFHDPWYFLKRQVAWLGVGLLVMHLISRIDYTIWKKLAIPLLCGATL
                     LLVLVLIPSLGNVAKGARRWLHLGPINIQPAEVAKFVAVIYAAAYLTKKQDQITQFAR
                     GLLPPLIVIGLLSGLVLLEPDLGTVVVMGLVVVTLLFLAGARIKHLAILSLCALTGVA
                     GLILTSPYRWKRFLMFLDPTKDPSGAGFQITQSFLAFGSGGPFGVGLGEGKQKLFFLP
                     EAHTDFVLALVGEELGLLGTVTIVLLFGLFVIKGFQIAGRARNAFGKHLAMGITMLIG
                     MQALVNAGVVTGLLPTKGLTLPFVSYGGSSLVANLFGVGILLNISRDRQGGHGGQEGG
                     GSRGARKRGVITE"
     gene            14304..15416
                     /gene="murG"
                     /locus_tag="PLS35_06080"
     CDS             14304..15416
                     /gene="murG"
                     /locus_tag="PLS35_06080"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNI18259.1"
                     /translation="MIAAGGTGGHLYPAIAVAREFLRRDPSTRILFVGTTRGIEQKVL
                     AHEGLPLRCITAHPFMGKSPVEMVKALITLPVSLWQSLGVLKQQSADLVFGVGGYTSP
                     AMLVAAFLSRVPGVILEPNAYPGLANKAVAPLVQRIFLAFESTKQFFDRRKTSVVGVP
                     VRQAFLDSSSPETARSETGPKRHLLIFGGSQGAKAINSAVIDALPMLATMNTRLTITH
                     QTGEADHARVAAAYQQAGISAQVVPFLYDMPTALRAADLVVARAGAMTIAELTVCGKP
                     AILIPLPTAIYNHQLRNAEVMAKAGGAVLLPQAELTGAALAHAMTEIFSDQQRLETMS
                     RHSWNMRRSDAAETIVRECYDVIRRRHEASDSARAL"
     gene            15385..16881
                     /gene="murC"
                     /locus_tag="PLS35_06085"
     CDS             15385..16881
                     /gene="murC"
                     /locus_tag="PLS35_06085"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNI18260.1"
                     /translation="MRPATALAPYEQRERERQQQDRNSGQRSFRGQELRRTAMFRKTQ
                     HIHLVGIGGSGMSGIAEVLLTLGYKVSGSDLSQSETTRRLEELGGRIAIGHHEANIGE
                     AQVVVISSAVAATNPEVVAAKARQIPVIPRAEMLAELMRLKFGVAIAGAHGKTTTTSM
                     VANVLAQGGLDPTMVIGGKVNALGSHARLGRGDLLIAEADESDGSFLRLAPTIAAVTN
                     IDREHLDHYGSMERLNDSFLEFVNKVPFYGLAVLCSDDERLRNLLPRVVKRFQTYGLN
                     EYPDHVPDFRATDIALRQWGSEFRVFFRGRNLGPFRLAIPGIHNVSNSLVAIAIGMEL
                     DIPVDLIRKGLAAFSGVERRFHLRGEKAGIMVVDDYGHHPTEVRATIAAAKQGWDRRV
                     VVLFQPHRYSRSRDLMQEFSHAFDQADALFMTEIYAAGEQPIPGVSGAKLVEAVKAAG
                     HPSATFVERKETLAEQVLPTLKSGDLVITLGAGDIWKAGLGILDRLPA"
     gene            16903..17835
                     /gene="murB"
                     /locus_tag="PLS35_06090"
     CDS             16903..17835
                     /gene="murB"
                     /locus_tag="PLS35_06090"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNI18261.1"
                     /translation="MSRVARKEQSARTRKDWARILEGIRGTITYEASLQSYTSFRIGG
                     PAEVLVEPADVDDLCRVVAQAHAERLPIFVVGGTNLLVRDGGIRGLVLSLRQLKAIRQ
                     EPGSVLYAEGGVGMPTLIGYAIRRSLAGLEWGAGIPGTVAGCVVMNAGTRLGEMKDSV
                     KAVRMVDPKGRVMDIPAAEIPFSYRKAHLPRGIVAAVWLQLKPGAHDQIEKTVKEYLQ
                     YRKNTQPLTLPSAGCVFKNPPQDSAGRLVDVAGLKGARVGDAQVSEKHANFMVNLGHA
                     RAADVLALIRKVRAGVKKASGVTLELELKVVGQA"
     gene            17845..18843
                     /locus_tag="PLS35_06095"
     CDS             17845..18843
                     /locus_tag="PLS35_06095"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNI18262.1"
                     /translation="MKGVHVTERKPLTRSKIGVLMGGQSSEREVSLKTGEAVYRSLVR
                     SGYDAVAIDVGPGLSQTLQDQAIEVAFLALHGPGGEDGAIQGFLETLGIPYTGSGVRA
                     SAIGMHKVVTKTELAAQGIPVPRGTVVWRGTAPTLNRILTSCKLKLPIVVKPASQGST
                     IGVTIVRQPSQWKEALKVAHRYDPEAMVEAFIPGHEVTLSLLGTADGSVAGLPAVEIV
                     APDGFYDFSAKYEKGRTQYLCPAPLPAAVSRDIKQLAIRTYQALGCNGAARVDFRITP
                     KGKPYVLEINTVPGMTETSLLPMAAGKAGLSYDALTEQILQSALRRAGTGSFRAVR"
     gene            18846..19715
                     /locus_tag="PLS35_06100"
     CDS             18846..19715
                     /locus_tag="PLS35_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNI18263.1"
                     /translation="MPLRWRNTRPAKLNPRANARSESGLERRRGGATGGYWTRLGRGL
                     LITLRVIATVTVLVGGCSGLFVLAREIGPLTREWFLVRSVSVNGLHHVTRKEVIGRLA
                     LKPDTALYSINPTWLAERIKTHPWIKDATVVLKPLHEIHIDIVEREPAVVVRTLAENL
                     LTDAEGVLLARLGSSDDPTLPILSGVDGKRLAQGRAEDRRPVQVGAALARMVGQTTGG
                     RPDINVGNLNNLVVEVQGVTFQFSESSMNQQWSRFLKMRPALRDVAFDGEGARANEID
                     LRFADRVIVRGRG"
     gene            19721..20956
                     /gene="ftsA"
                     /locus_tag="PLS35_06105"
     CDS             19721..20956
                     /gene="ftsA"
                     /locus_tag="PLS35_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNI18264.1"
                     /translation="MSKRDHILVGLDIGTTKICAIVAEVPEEGPLNIIGVGSCPSRGL
                     RKGVVVNIESTVESIKKAVEEAELMAAVQINSVYTGIAGSHISGENLKGVVALKKQEV
                     TRDDISRAVESARTLAVIPHERRILHVLPREFMVDDQEGVREPLGMSGNRLEVNVHVI
                     TGAVTSAQNIIKSVNRAGLDVVDIILQPLASSEAVLSAEERELGVAMVDLGGGTTDLA
                     IFLDGSIRHTAVLPIGGQNLTKDLAIGLLTSQTDAEKIKVQHGIARTELVHGHQMVEV
                     PSVGDRPPRQFTRRDIAEILEPRVEEMFDLVKREIVRAGYEGMLGAGVVITGGTSLLE
                     GMPDAAERGLNLPARRGAPTGIGGLRDIVSNPMHATGVGLLLHARQHADNLEAAGIRH
                     GKGLGKVFDRMRSWMFEFF"
     gene            21009..22211
                     /gene="ftsZ"
                     /locus_tag="PLS35_06110"
     CDS             21009..22211
                     /gene="ftsZ"
                     /locus_tag="PLS35_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNI18265.1"
                     /translation="MFSFQEEPQSPVRIKVIGVGGAGCNAVNTMITGGLCRVDFVAAN
                     TDVQALERSQASYKIQIGPERTRGLGAGAKPEVGRDAALESKDEIRESLVGADMVFVT
                     AGMGGGTGTGAAPIVASIARELGILTVAVVTKPFQYEGHRRMSHAEEGIRDLGRHVDT
                     LLIIPNQRLLGIVDKATPLLDAFKVADDVLRQAIQGIADVITTTGLVNVDFADVRTIM
                     AHTGRAVMGMGIGRGANRAQEAAQKAICSPLLEEGSVEGARGVLLNITGGPNMSLHEV
                     EEAASIVQHAADAEANIIVGQVINPEIGDDLIVTVIATGFEREEQPAARPAAAAERPA
                     ARTPNGRPAQQVLTGVHATGSDRPIKDIDRPTFLRRMGETREAVERIAVVGDDEWDVP
                     TFLRKQAD"
     gene            22252..>22745
                     /locus_tag="PLS35_06115"
     CDS             22252..>22745
                     /locus_tag="PLS35_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNI18266.1"
                     /translation="MASEFITLPSFATHADGAEHFFGTRLSAIPVRPGRASAHRTEQQ
                     DHRAPVILSVKQVHGTDALVVDRPVEQGELFEGGWDALVTNQPGLMVTVRTADCVPVL
                     LHDPVRQVVAAIHAGWRGAVAGIVPKTVALLASRFGTVPKDVRMAIGPSAGACCYEVD
                     EPVLT"
     assembly_gap    22746..22845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22961..23686
                     /locus_tag="PLS35_06120"
     CDS             22961..23686
                     /locus_tag="PLS35_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNI18267.1"
                     /translation="MDSELGTIAGRVRAVFDEIHRAAVRAGRAPDSVRLLAASKTVTV
                     ERLREAVDAGIRHLGENRLQEALPKIETLDREGVVWHFIGTLQRRKVKSVVGRFETIH
                     SVDSLALAEEIDRQAKAAGLKQRVLLEVNLGGESSKGGFAPAAVAAALPALNQLEQLD
                     IRGLMAIPPPMPTAEDARPYFRQLRELAQALTAQGCRNINMQELSMGMSHDYAVAIEE
                     GATYVRVGTAIFGARSEPYHDHT"
     gene            23673..>24415
                     /gene="proC"
                     /locus_tag="PLS35_06125"
     CDS             23673..>24415
                     /gene="proC"
                     /locus_tag="PLS35_06125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNI18268.1"
                     /translation="MITRNIAVLGGGQMAEALIGGLLASKVSEPQFICATDPVSARRD
                     LLKSRFGIQVGEDNAKAVAGADLVLLAVKPQVLPAVLQDVSSALRGKLVVSIAAGVTT
                     AWIQERVSAARGIVRAMPNTPALVREGVTALAYAADLAADDVAAVRALFEAVGSVVSV
                     EERLMDAVTGLSGSGPAYVFVAIEALADGGVKMGLPRATAQLLAAQTVLGAARMVLER
                     GQHPAQLKDQVASPGGTTIAGLHQLEVGG"
BASE COUNT         4785 a         6475 c         8028 g         5027 t
ORIGIN      
        1 tagaaggagg acatcgtggc tgaccaatca tcattcgatg tggtgtcgga agtgaatatg
       61 caggaaatga aaaacgtcgt cgatcaggcg acgaaagaga tcaagcagcg attcgacttc
      121 aaggattcca agaccgagct gacgctaaag gaaaaagaaa aagaactcgt cgtgctctcc
      181 gacgatgagt acaaactcaa tgccgtcatt gaaatcatca agagcaagtg cgtgaagcgc
      241 ggcgtctcct tgaaggcctt cgagtacggc aagatcgagg aggcgctcgg ggcgaccgtt
      301 cgtcaagtga tcaagattca aagcggcatt tcctcagaaa aggcgaagga aattaccaag
      361 gcggtcaaag aatccaagtt gaaggtgcag gcgcaaattc agggtgaaca ggtgcgggtg
      421 atcagcaaga gcaaagacga tctgcagtcg gcgatcgcct ttctcaaggg gaaagatttt
      481 gggattgatc ttcagttcac caactaccgc tagcctgggg atggtgaaac aggtgcccag
      541 cttcgttctc gcttcgatca gaggctcaac gtacaagcac agtacgcctc gcctcttccc
      601 tcgctgcggc ctcgctgagc gacctgtttg accatcccct gtggatccgc ggttcaagca
      661 tgaccaaatt tcttgcctct atgttcctgc ttgccggtct cttgctcggc ggatgcggtg
      721 gcagtgaccc ggtcgttgtc atccaactcc atcccaccaa ccccgacatt ctgtacatcg
      781 ccaccaacga ctacatttac aaatcgcgtg acggcggcaa gacgtggagc aatctctcga
      841 aggggatgag ttactcgcgt gtgattgcga tggcggttga tcccgtctat cccgccacaa
      901 tctatgcggg cacgaagggg gatgccgtct acaaaagtta tgacggcggc cagcgctgga
      961 cctcggtccg gaccggattg gatgatgcga cgatatcgtc cgtcgtgaat caatttgtct
     1021 tcgatccctc agacgataac cacatcttcc tggcgacgac gatgggtgtg ttcgaatcca
     1081 agaatgccgg ggagcggtgg gtcaagagga tggagggcat gaaggaagtg ttgatggtcg
     1141 tgacgctagg catggacccc gcgcatccct ccgtaatgta tgcgggaacc agtggaggcg
     1201 tgtacaagac gaatgaccaa ggggcgcatt ggctacaggt gaacaacggg ttggtgccgg
     1261 aaggtatggt gaagacgtcg cgtgcgctcg gtgtgacgtc ggtgcaagtc gatcctttcg
     1321 cgcccgacac ggtctacgcg gcgacgctgg ccggcctgta taaaaccacg gatggcgcag
     1381 tgtcctgggt gcgcatcggt gagtcattac aggatcagat gattgtggca atggtgctgg
     1441 atcatgcgca ggccggcgta atgtatctgg ccgggcggga tggtattcac cggagcggcg
     1501 atggcggcaa gacctggata gtgctgaatg aaggcctgac aaccaccaac atccgttcat
     1561 tggcccagag tgccatggat ccacacgtgc tcttcgcggg cacgaacggc agcgggctct
     1621 acaagagtgt ggatggcggc gcccattggc aggcgatgcc gtcggtattt ccaaaggact
     1681 agtaggtggt gtcgctaagc tggaggcggt tggaaagcga ggctatcggc gcgattggct
     1741 gttcaaggaa gagtcaccga tggatggacc gactcctcga atgtgcgcag aggaggactg
     1801 ctcggcattc tcggccgcat tgatctcaaa gcctgatgca ttcggcaagg tcgtgtcttg
     1861 cgtctgagta ccgacacgat ccgttaacac gctatcccta agatcttccc ctctgcgtgg
     1921 acggagcgat tcgttcacat tgctgtaggg cgagaccgtg gtgccgatgt ccgcgccgaa
     1981 gctcggattg agtgaacggt ttaaatgcgt gttctcgatg tcgccgccga tcctggtcgg
     2041 gatggtggtg tcctggatgt ccatgccggt tggcgcggag gttgccggtc ggtttggtcg
     2101 aagatcgtgc gtaatcgcct cgcgttcatc ttcaatgcgt cgttcttctc tcccgcgctg
     2161 ttcgtaatcc agtgagtcga gtttttcata tcgcgtgatg aggcccttca tccgggtcct
     2221 gagttttttg aggtcatctc tcgcgatggc gacagtgccg acaatttcgc tgagcgtcca
     2281 ttgccgggca aaggtgccga gtttgggaga ctggccaggg ccgccgccca aaccgctcgc
     2341 gccgcttccc acgccgaacc ccgcgctgtt ataaatgccc ttttgttgaa ccgacatcaa
     2401 aatgtgattg gactgttcga gaagttcgtc gatgcgatcg agggaatccc attcgctcgc
     2461 gcagcaatcg ataaattcac ggtagtcgcg ggtgaacttc gaggcttcat tttgaatatc
     2521 gaggatcttg agcggttctt ttgttgcggc atcgttgcgc tcgtcttggt tgagtgcggc
     2581 gggctttttc tcatcccgct ctgcatcgta cagcgcagtg caacccgcat caggggtggc
     2641 ggtaatgcgt ttgtcggcag cgcgattcgg acaccaatag atttttccgg gctgattgag
     2701 cggattgtct gacttgaatt ctcccgcctg aaccgcaggc aggagtccca ccagcaggag
     2761 cccgatgagc gctgacgcga gccaatgccg agggtgtagc gtacttgtct gtatcatggt
     2821 tttgatgatg ccccccgaga gacattgtgt caatcaaagg ggagaattgt gatgcgcttg
     2881 aaaagcccga ggccgttcgt taccctcccg cataatcgaa gtctctcacg ttcatagcca
     2941 gggggaatgc ccatgcccgt gatctatatc gacgaagctg cgacccatgc aggtcaggaa
     3001 gtcacgattc gcggatggct ccgcagtcga cgcgacaagg gaaaacttca ctttctcatt
     3061 gtgcgggacg ggacgggcga tatacaggca gtcgtgagca aaggcgcagt cggagacgag
     3121 cagtttgccc attcagccga gctcacgcag gagtcgagcg tggtgctgac cggcacgttg
     3181 cggcaggatg cgcgcgcacc gggcgggtac gagcttgatg tgaagcgcct cgctgtcttg
     3241 caggtggcgg agccatttcc cattcagccc aaagagcacg gtacaggatt tttaatggaa
     3301 catcggcatc tctggctgcg ctccagtcgc cagcatgccg tgctgcgcat tcgtcacgag
     3361 attattcgcg cctgccggaa ttttttcgat gatcgcgggt tcacactggt cgatgcgccg
     3421 atttttacgc cgaatgcctg cgagggcact acgacactgt ttcagacgca atattttgat
     3481 gagacggcgt acctgacgca gagcggccag ctctatagcg aagccactgc cgccgccttt
     3541 ggaaaggtct attgttttgg tccgacattt cgcgcggaga agtccaagac gcgccggcat
     3601 ctgatggaat tctggatggt ggagcccgaa gtggctttcg ccgaactgcc cgacatgatg
     3661 gatctcgccg agcagttttt ggcgcatatc gtcgcggctg tgctgaagac gcgtcgtgcc
     3721 gaattgcaga tcttggagcg ggatatcgcg aagctggagc gcgtgacggc gccgttcccc
     3781 cgcattacgt atgaggaagc cgtggacatt cttcagcgga aaggaaatcc cgccaaacga
     3841 ggagatgatt ttggcgcgga cgatgaaacc attttatcga aagaattcga ccgccccgtc
     3901 atcgtgcatc gatatccggc tgcgctcaaa gccttctata tggagaccga tccggctcgc
     3961 cctgatcttg ccctgtgcat ggatgtgttg gcgcccgagg ggtatggcga aatcatcggc
     4021 ggcgggcaac gcattcacca gcacgagaaa ttgttggcgc ggatacgcga gcaccatctc
     4081 ccggtcgaag cgtttcaatg gtatctcgat ctgcgtcgct acggctcggt tccacatgcc
     4141 ggattcggaa tgggaatcga gcgcgcggtg gcgtggattt gcggcctcga acatgtgcgg
     4201 gagacgattc ctttccccag aatgttgtac cggttatatc cataggtttt ccccatcctt
     4261 cccatcggct gattcctcct gcgctcaccg aaatgctgcc attccatctg tctgattttt
     4321 tggacgggtc tcgcgtttca caaccatttt catgccgcaa ttaatcgaag ggtgggatag
     4381 tttcccacag gtttgtccac aaatgtggat aactttgctg ccaaattgcg gcctgcaaac
     4441 gatgggtcaa aagcgaacaa tattcttgat aaattggagc aaaatctgtt gacagctgtt
     4501 ttggtgggtg tatactccag ccaacgtgtg gtgaaaagtg ggtggaagtg ggtgtatgga
     4561 ttctgctcgc ggttcccatg agccggtttt agtcgacgaa atcgtattct ggctgcagtg
     4621 caaaccaggt ggagtttacg tggactgcac tcttggatat gcagggctcg ctacccggat
     4681 ccttcatcac accgctccag atggcattct cattgggatc gatcgcgatg cggatgcgct
     4741 ggaagaatcc cgcgcgcgcc tgcaaggcgt catgccccga gtacatcttc ggcatggaaa
     4801 tttttcgcaa ctcaaaacac tcgtggcgca ggtcggggtc cagcatgtgg atggtgtgat
     4861 tttcgatctc ggtgtgtcct cgccgcaact cgatcggccg gagcgcggat tcagttttcg
     4921 tgacgatggc ccattggaca tgcgcatgga tcggagcgaa ggacgcacgg cagccgacct
     4981 tatacgagag atgcctgaac gtgaattggc ggatgtgatc tatcaattgg gcgaggagcg
     5041 gtattcgcgc agaatcgccc gcgcgatcgt ggaggcgaga acgcaacggg tcatcgggac
     5101 gacgagggaa ttagcggcgg tggtggagca tgccgtgccg gcttcgtacc ggcatgggcg
     5161 aatccattgc gccacgcgga cgtttcaggc gctacgaatc gcggtgaacc gggaattgga
     5221 ggtgatcgaa ccggcgcttc gcgatgccgt cgatctagtg gcccccggag gacgagtctg
     5281 cgccgtgtcg ttccattctc ttgaggacag aattgtgaaa catacgtttc gggctttggc
     5341 gaatggcccg gacgctgcag tgagggtgct cacaaaaaaa ccggtcatcg cgtcggagag
     5401 cgagcgtgac cataacccgc gttcgcgcag cgccaagttg cgtgtcgtgg agcggatgtc
     5461 caaggagtct atgcaatgaa agccgtcgcg tttgccgcgg tcacgtcgtt ggtgttgctc
     5521 ttcgtgtggg aacgggtgga catcgttcgc atcggatacc acatcgagcg gctgaaagcg
     5581 caaaaagtgc tgctcgagcg tgagcgggat gaattgcaag tcaagctgtc gggtctcacg
     5641 gcgcctgaac ggattgcacg cttggccagc gataaattgg gtatgttgca accggagaag
     5701 ggccaggtgg tcgtcatcaa ctttgagccc gaagcgcctg cgaatcccgt tgccgcagaa
     5761 ggcgaaattc ggatagcgaa gaatatggtc acccggagag cgcgatagtg gcttcgccct
     5821 cctttcgtgg tcgccggatc ctggtggcct gcgggttgtc tggtgcgttt atcctcgtga
     5881 ttgtccgcct ggtgaacctt caggtgatgc aatcggcgga gttgaccgtc aaggctgacc
     5941 ggcaacatca aaaaaatgtc acgctggagg gggcgcgcgg caccatctac gatcggaaca
     6001 gcaaagtgct ggcgatgaac atggatgtgc cgtctgtgtt cggggtgccc gcttcgctcg
     6061 gaaatccagg agtggccgcc cgcaatctct cgccgatctt gcatgtgaag gcaaccgaac
     6121 tcgaaaaaaa gttgaagcag gaaaagcatt tcgtgtggct tgccagaaag ctggaaccag
     6181 agcagggacg gcggcttgaa cggcttggcc tcgatggcgt cggtgtggtg atggaaggcc
     6241 gtcggttcta cccgaagggc ccgctgctgt cgcatgtgct gggatttgcc gggatggatg
     6301 atcgtggcct ggaaggagtg gagctgcgat acgagcagta tctgcgcggc gaaaaacgtg
     6361 ccgtggtgtt gcagcgagat gccttgggtc gcgcggtttt ccccaaggga ttgaatgaag
     6421 aaggcgccgc cgccggacat agcctcacgt tgacggtcga cgaggtggtg caatatatcg
     6481 ccgagaagga gctggatgag gcggtgacac gatccagtgc caagtccggc acgatgattg
     6541 tgatggatcc ccggagcggc gccgtgctgg ctatggccgt gagtccgcga ttcgatccca
     6601 atacggtcgg cgccctggct cccgaccgat ggcgtaaccg tgccctgacc gacacctacg
     6661 aaccgggctc gaccatgaag gccgtcattg cagcggcggc gctggaagaa aaggtcatga
     6721 ccccgggcag tatgatttat ggtgaaaacg gacaattcgc gattgccaac acgatcatcc
     6781 atgaccatga aaaagccggc tggatgacgt ttgcccagat gattcaaaaa tcgagcaata
     6841 tcggcgctgc caaggtcggg atggcgttgg gtgaatggcg ggtgttcgac tatttgaagg
     6901 agtttggctt cggagataag agcggcattg atctgccggg tgaaacggcc ggattgttac
     6961 gagggcctcg ccaatggggc aaacgctcgt tggcctccat ttccatgggg caggaagtgg
     7021 gtgtcactcc attgcagatg gtgacggcaa tttcggcgat cgccaatggc ggcgttctca
     7081 tgaagccgta tgttgtctcg gagatccgta acggcaaagg gcaattggtg gcgcagaata
     7141 tgccgcaagc caaacgtcgt attatttctg ccgacacggc gcggacgttg acgacgcttc
     7201 tggaaggagt ggtcacccac ggaacaggga ccaaggcagc tatccccggt tttcgtgtgg
     7261 ccggcaagac cggcaccgct caaaaagtcg acccccgaac cggcaagtac tcctccacgc
     7321 tcctggtcgg atcgtttctc ggctatgttc cagcggaaga cccgcgactg gccatgattg
     7381 tggtgattga cgaaccgcgc ggcgaaggct ggggtggtgt ggtggcggct ccggtgttcc
     7441 ggcgggtcgg cgagcaggtg ctcaattatc tgggtgtcgc agtcgatgaa ccggtcaagt
     7501 tggcgatggc ggggctggaa tcctgatatg acgttggatg agctgatcag tcccattcag
     7561 gggcgccttg gagtgctgga gcggagcgga gatcagcgcg tggccattga cgcgttgacc
     7621 gatgattccc ggcacgtcca gtccggtgcg ctctttgtgg ccgtgcaagg agagcgggtc
     7681 gatggccacg agtttctaga ccgtgtcgtg aaggcggggg ctgcggccgt cgtcgtgggt
     7741 cgccacgttc aggtcggatc gacgccatgt atccgggtgc aggattcccg ggcggcgttg
     7801 ggaatggtgg gaagccgatt ttatggagat ccttctgctg cgctccgcat ggtcggagtg
     7861 acggggacca acgggaagac cacgacgacg tatgtcgtga aaaccatgct ggaggccgcg
     7921 aatcggcagg tcggtctcat cggcacggtc gcgtacctcg tcggaaaaga atccattccg
     7981 gcgtcgcata ccacccccgg tgcgttggag ttgcagaaat tatttgcacg aatggtcgag
     8041 aagaggttgg atacggtcgt catggaagtg tcgtctcacg ccttggccct tgatcgcacc
     8101 gccggctcgg aattcgatgt cgccgtgttt accaacctca cgcaggacca tttggacttc
     8161 catctggata tggaacggta cttccaggcg aagcgcaagt tgttcgccga gctcggccag
     8221 cctgactcgc gcaagacaaa aaagcgggcc atcatcaatg cggatgatcc ctggggtgca
     8281 cggatgcgcg aagcctgcac ggtcccggtg tggacctatg ggttgcatca acaggccgac
     8341 atccatgcgg acgaggtgac attgtcgcct gcgggcacca gttttacgtt gcgcaccccg
     8401 gccggtacat gcccgattca gagtcgcctg gtcggagaac acaacgtctc gaacctgctt
     8461 gccgcgatcg gggtcgtgct ccacgaagga ttaacactgg agcaagtccg tgccgcggtg
     8521 ggcgctgtct ccaatgtacc ggggcgattc gaacgggttg acgcaggaca ggacttcacg
     8581 gtggtggtgg actatgcgca taccgaagat gccttggtcc gattattgac cgcggcccag
     8641 gcgcttcgca cggggcgtat cattaccctg tttggatgtg gtggcgaccg ggatcgcacc
     8701 aagcgtccca agatgggtcg cgctgcggtt cagtatagtg acgtggtgat cctgacatcg
     8761 gacaatccgc gcacggaaga cccggcgtcc attctcagcg aagtcgaggt cggggtgaag
     8821 gacgcgctga cggctcgcag tcatgttcga tatcggatga ttgcggatcg acgcgcagca
     8881 atcgaggctg ccatcaacga ggcgaaggcc ggcgatatgg tgttgattgc gggaaaaggc
     8941 cacgaggact atcagatcgt ggggacgacg aaacatcact tcgacgatcg ggaagtggcg
     9001 cgtgagatga ttcaggcgcg ccgatcctga cgctcagcga ggcatgacat gcgggagcat
     9061 ggacatcgtg gcgtaatggc gttgtttaca gtcgaagaaa tttgtgaagt gttgagcgcc
     9121 aagtcgccga ctggcctgag tccgcaagat ttgaagcagc ggatccgccg ggtggtgacc
     9181 gattctcgtc tggtgcgcaa aggtgatctg ttcatcgcct tccagggaga gcgattcgac
     9241 gcgcatacgt tcgtgccgaa agtcttcgcg caaggcgccg tttgtgcgat cgtgcaggag
     9301 gactaccgcc ttccgccgat gaagccgcga accggcgcac cgattgtgct cggcgtgagg
     9361 gatacgctcg aagcctttca gcggttggcg acgcactatc gcaaccggtt tgccatcccg
     9421 gtgatcgcca tcacggggag caacgggaag acgacgacga aggagatggt cgctcatgtg
     9481 gtcgcacagc ggtggaaaac gctcaagacc gagggcaatc tgaataatcg catcggcgtg
     9541 ccgcagacat tgttccagct ggcgccgcgg catcaggctg cggtgatcga aatgggcgtc
     9601 gatcagcagg ggcaaacgac ccggctgtgc gaaatcgcga gaccgacggt cggcgtgatt
     9661 accaatatcg gaccggacca tctggaattt ttcggcagca tggaagggtc cgcgcaagcc
     9721 aaggccgagt tgctcgatca tctgccgcaa gacgggtcgg tggtgctgaa cgccgacgat
     9781 gagtatttcg attatctggc tgcccgtgct caatgccggg tggtcgcgtt tggttcgtca
     9841 tccaaggcag ccgttcgtgc cgcgaatgtg cgaaccgatg acaagggggg cacgctcttc
     9901 ggactggtgc ttcccggaaa gacgcgtcag acggaggttc gtatccgaac ccaggggcaa
     9961 cacaacgtga gtaatgccct ggcggcggcc gccgtgggac atgccttggg gctgtccggc
    10021 gcggctatcg ccgaaggatt ggcgaagttt cgtccggccg cgatgcgatc acagatcagt
    10081 tcttcgcatg gtgtgcaggt catcaatgat tgttacaacg ccaatcccgc gtccatgaag
    10141 gccgccatcc agctcctggc gcaattgggg cgtgacaaac gatcgatcgc ggctctgggt
    10201 gacatgctgg agttaggggc ggataccaaa cgcatgcatc gggacgtcgg agcgtttctc
    10261 gccgagcaag ggatcgggca cctgatggcc tgtggtgtct tgggacggga gctggcggcc
    10321 ggtgcacgtc aagcgggcat gccggctgat cggattacag aacatccgga tgcccaggcc
    10381 gcagccacgg ctttggcacg gatggtgcga cagggcgacg tggtgttggt caaggcgtcg
    10441 cgcgggatgc ggatggaaca ggtcgtcgac gcgctgaccg gcatgcgacg cgtggcgaaa
    10501 aaggcctgtt agccacactt aaaggaattt tagaatccgc cgattttggg cgggcaaaga
    10561 agccgtagga tgttatacaa ctggctctat ccacttcata cacagttttc gttcctgaac
    10621 gtctttcgct atcagagttt ccggatcatc tacgcagcgg tcaccgcgtt tctcatcgcc
    10681 tttgtgatgg cgccctgggt catccggaaa ttgcaggaga ttaaactcgg ccagcagatt
    10741 cgcgacgacg ggccgaagcg gcacttggcc aagagtggaa cgccgacgat gggcggcatt
    10801 ctcattatct ttgcggtcgt gctctccacg ctcttgtggg ccgacatgac caaccgctac
    10861 gtgtggttgg tggtggtggc gaccgtcggc ttcggcgccg tgggatttgc cgacgactat
    10921 ctgaaattca tcaagcgcca atccaaagga ctgtcggccg cgcaaaaatt ttccggccag
    10981 ttcctcgtgg cgttggccat cggcgtcttt ctctacaccc tcccgagcta cacgaccaaa
    11041 ctcagcgtgc ccttcttcaa gttttttacg ccggacttgg gctggttcta cattgtgttc
    11101 gtcatccttg tcatcgtcgg cagttccaat gccgtcaatt tgacggacgg gctcgatggg
    11161 ttggcgatcg gccccgtcat gatcgcctca ctggcctata cgatcgtcgc gtatgtgaca
    11221 gggaatcggg tcatggccga atatttgctt atcccgtaca tcgaaggcgc cggagaaatc
    11281 gctattttta cgggtgcgat tttagggtcg agtctgggat tcctttggtt caacacgtac
    11341 ccggcttccg tattcatggg cgacgtgggg tcgctcccct taggcgcggc gctgggaacg
    11401 gtggccgtga tcagcaagca tgaactgctg ctccttttgg tgggcggagt gttcgtcatc
    11461 gaagccttgt cggtcatctt gcaggttgga tcttataaat tgcggggcaa gcgcattttc
    11521 aatatggcgc cgatccatca tcactttgag atgaaaggat gggacgagcc gaaggtcgtc
    11581 gtgcgcctgt ggattattgc cattttgttg gcgttgctca gcttgagcac gctcaaattg
    11641 cggtagacgg aatcgtatgg acgtcaaggg tcttcaagtc acggtcgttg gtctcgccag
    11701 gagcggcgtc ggcgctgcgc ggctgttgaa ccatcttggg gcacgggtca ccgtggccga
    11761 ccggaaagag ccgcaggaac tcgcgaccat cctctcccag ttggatcagt cgggcattgc
    11821 cgtcaaggtg ggagcccagt atgaatccgc cctcgagggc gcggatctgg tcgtgatcag
    11881 ccccggagtg ccgacacagc tggatgcgct caaccgtgtg cgtgctcgcg gggtgcgagt
    11941 gatcggcgaa ctggaattgg cgtcacggta tatgaccgcg ccgatcgtgg cggtgaccgg
    12001 aaccaacgga aaaagcacca cggtgacgtt gatcggcaag tttctgaagg aaagcggaaa
    12061 gcgagctttc gtggggggca atctcggcat cgcagccagc gaagcggctc tcacgtgtgt
    12121 gcaggccaag cccgggagcc ctccgcctta tgagtatgtg gtcttggaaa cctcgagctt
    12181 tcagctggaa acaattgagc agttccatcc gttcattgca tcgatcttga acgtgacgct
    12241 cgaccatatg gaccgttaca actcggtaga agattatgtg gcggccaagg cgcggatttt
    12301 cacgaatcaa acggccggtg actattccct gttcaatctg gacgacacgc gagtcgctgc
    12361 gctgcgcggg aatacagaag ctgcggttgt cggcttcagc cggagtggag ccacggtttc
    12421 cggcgtggcg ggcgccccgg tgctggacgg tgatctgatc gtcacgacgg tgcggggaca
    12481 gcgcgaggaa atctgtcgcc ggggcgacat gcgattgatc ggactccaca atgtggaaaa
    12541 cgtgatggcg tcagtgacct atggtctgct gtgcggctgt tccatcgaag cgatccgcgc
    12601 cgtactgcga ttctttcccg gcctggagca tgccttggaa gtggtacgtg agcgccgggg
    12661 cgtccggtac gtgaatgatt ccaaaggtac gaatgtcgat gcggtattga aagccctgga
    12721 aggtatcgaa cagccgatct ggttgatcgc cggcggacgt gataaaggcg gtgatttctc
    12781 ccgtcttgaa ggagcgattc gtgaacgggt gaaggggctc attctaatag gcgaagccgc
    12841 agcccgcatc caagcggcga tgggtgattt tgaccggtgt cgtcctgcag cgaccctccg
    12901 cgatgccgtg gagcttgctg ctcgcgaggc gcagcccggt gaggtggtcc tactctcgcc
    12961 ggcctgcgcc agttttgaca tgtttgcgga ttatcaagac cggggccggc aattcaaggc
    13021 gctggtccag gcgcttccgg cgtgagaccg ggagtcgcgc ggaggaacga caactgaacg
    13081 atggcacagc atgcgctcgg gactctgatg ttgccctggt cgactacgac tcagcgggcg
    13141 tccaagcgcg tgccggtgga cccggcactg ttggcggtca ccctgatcct ggccctgatc
    13201 ggtgtggtga tggtgttcag cgccagtgcc gttgtggcgg gcaaccggtt tcacgatccg
    13261 tggtatttcc tcaaacggca ggtcgcctgg ttgggcgtcg gcctgctggt catgcatctc
    13321 atctcacgga tcgactacac aatctggaag aagctggcga tccccctgct ctgcggggct
    13381 acgctgttgc tcgtgttggt gctcattcca tcgctcggga atgtggccaa gggtgcacgg
    13441 cggtggctcc atctggggcc gatcaatatt cagccggccg aggtggcgaa attcgtggcc
    13501 gtcatctatg cggctgccta tctgaccaaa aagcaggatc agattacgca attcgcgcgg
    13561 ggcttgttgc cgccgctgat cgtcattggt ttgctgagcg gcttggtgct gctggaaccg
    13621 gacctgggca ccgtcgtggt gatgggactc gtggtggtga cgttgctgtt tctggccggg
    13681 gcgcggatca agcacctggc gattctctcc ttgtgcgcct taactggggt ggccgggctc
    13741 attttgacgt caccgtaccg ttggaaacgg tttctcatgt tcttggatcc cacgaaggat
    13801 ccttccggag cgggctttca gatcacgcag tcgtttttag ccttcggaag cggtgggccg
    13861 ttcggagtcg gtttgggaga gggcaaacag aaattattct tcctgccgga ggcgcacaca
    13921 gattttgtgc tcgcgttggt cggcgaggag ttggggttgt tgggcaccgt gacgatcgtg
    13981 ttgctcttcg gtctcttcgt gatcaaaggc tttcagattg cgggccgggc gaggaatgca
    14041 ttcggaaaac atctggcgat gggcatcacg atgttgatcg gcatgcaggc gctggtcaat
    14101 gcgggtgtgg tcacgggact gttacccacc aaggggttga cgctcccctt cgtcagttac
    14161 ggcggctctt cgctggtagc caatctgttc ggtgtgggga ttctgttgaa tatctcacgc
    14221 gaccggcagg gcggccatgg cgggcaagag ggcggcggat cacggggcgc gcgaaagcgc
    14281 ggggtcatca cggaatgaac atcgtgattg cagcaggagg cacagggggg catctgtatc
    14341 cggccatcgc cgtggcgcgt gaatttttgc ggcgagatcc atcgacccgc atcctcttcg
    14401 tcgggacgac gcgtggcatt gagcaaaagg tgctggccca tgaagggctt ccgttgcggt
    14461 gcatcacggc gcatccgttt atgggcaaga gcccggttga gatggtgaaa gctctgatca
    14521 ctctgccggt gagtctttgg caatcgctcg gcgtgctgaa acagcagagc gccgatctgg
    14581 tgttcggcgt agggggatac accagcccag ccatgctggt ggcggcgttt ttgagccgag
    14641 tccccggcgt gattctcgag cccaatgcct acccggggct ggcgaataaa gccgtcgcgc
    14701 cgctcgtgca gcgcatcttt ctggccttcg agtccacgaa acaatttttc gatcgacgca
    14761 agacgagcgt ggtcggggtg ccggtgaggc aagccttctt ggattcgtcc tctcccgaga
    14821 cggcacggag cgagaccgga ccgaagcggc acctgttgat tttcggcggc agtcagggag
    14881 cgaaggccat caactcggcc gtgatcgacg cgttgcctat gctcgccacc atgaataccc
    14941 ggttgaccat cacgcaccaa accggcgagg cggatcatgc gcgagttgcg gcggcgtatc
    15001 agcaagcagg aatctctgcg caggtggtcc cgtttctgta cgacatgccc accgcgcttc
    15061 gtgccgcaga tctcgtggtg gcgcgggccg gagccatgac gattgccgaa ttgactgtct
    15121 gcgggaagcc cgcgattctg atcccgctcc ccacggctat ttacaatcat cagttgcgga
    15181 acgcagaagt gatggcgaag gcaggcgggg cagtccttct gccccaggcg gaattgaccg
    15241 gtgcggctct ggcccatgcc atgacggaaa ttttcagcga tcagcaacgt ttggagacca
    15301 tgagccggca cagctggaat atgcggcgca gtgatgcggc ggaaaccatt gtgcgtgaat
    15361 gttatgacgt cataaggagg cgccatgagg ccagcgacag cgctcgcgcc ctatgaacaa
    15421 cgagagcggg agagacagca acaggatcgg aacagcggac agcgatcatt tcgaggacag
    15481 gagctaaggc ggacggcgat gttcagaaag acacaacata ttcatctggt gggcatcggc
    15541 ggatccggga tgagcggcat cgccgaggtg ttgttgacgc tcggatacaa agtcagcggg
    15601 tcggacctca gccagtcgga aacgacgcgc cggcttgaag agctgggagg gcgcatcgcc
    15661 atcggccatc atgaggccaa tatcggcgag gcacaagtgg tggtgatttc ctccgcggtg
    15721 gcggcgacca atcctgaggt ggtggcggca aaagcgcgtc agattccggt cattccgcgc
    15781 gctgaaatgt tggccgaact gatgcggctg aagttcggcg tggcgatcgc cggggcgcat
    15841 ggcaaaacca ccacgacgtc aatggtggcc aatgtgctgg cacagggcgg gctggatccg
    15901 accatggtga tcggcggcaa ggtgaacgcg ctgggaagcc atgcccggtt ggggcgaggc
    15961 gatctgctca tcgcggaagc cgacgaaagc gacggctcgt ttctccgtct ggcgccgacc
    16021 atcgccgccg tcacgaatat cgatcgtgag catctggatc attacggcag catggagcgg
    16081 ctcaacgaca gtttcctcga atttgtgaac aaggtcccgt tctacggcct ggccgtgctc
    16141 tgctccgatg acgaacggct gcgcaatctg ttgccgcgcg tggtgaaacg attccagact
    16201 tacgggctga acgaatatcc cgatcacgtg ccggattttc gtgccaccga catcgctttg
    16261 cggcaatggg ggtccgagtt ccgggtgttc ttccggggac gcaacctggg gccgttccgg
    16321 ttggccattc ccggaatcca caatgtgtcg aattcgctgg tcgcgattgc gatcggcatg
    16381 gaactggata ttccggttga tctcatccgg aaaggcctgg cagcgttcag cggcgtcgaa
    16441 cgccggtttc atctgcgcgg ggaaaaggcc ggcatcatgg tggtggacga ttacggccat
    16501 catccgaccg aagtgcgggc cacaattgcg gctgcgaagc agggctggga ccggcgcgtg
    16561 gtcgtgctct ttcaacctca ccgctatagc cgctcacgcg atttgatgca ggagttttcc
    16621 catgcattcg atcaagccga tgcgctcttt atgacggaga tctatgccgc cggcgagcaa
    16681 ccgattcccg gcgtgtccgg cgccaagttg gtcgaagcgg tgaaggctgc cggccatcct
    16741 tcggccacct tcgtcgagcg gaaggaaacc ctggccgagc aggtgctgcc gacgttgaaa
    16801 tcgggggatc tcgtgatcac gctcggcgcg ggagacattt ggaaggcggg attgggcatc
    16861 ctcgatcgat tgccggcctg agggcgatga gaggcaggaa cggtgtcacg tgtggcgcgg
    16921 aaagagcaat cggcgagaac gaggaaagac tgggcgcgca tccttgaggg aattcgtgga
    16981 acgatcactt acgaggcgtc gctgcaatcc tatacgtcgt tccgcatcgg gggaccggcc
    17041 gaagtcctgg tggagccggc cgatgtggac gatttgtgcc gggtggtggc gcaggctcac
    17101 gccgaacgcc tcccgatttt cgtggtgggt gggacgaatc tgctcgttcg tgacggggga
    17161 atccgcggcc tcgtgcttag cctccggcag ctcaaggcca tccgccagga gccgggatct
    17221 gtcctctatg cggagggggg cgtgggcatg ccgacgttga tcgggtatgc catacgtcgc
    17281 tcgctggcag ggctggaatg gggcgccggc attcccggca cggtcgccgg ttgtgtggtg
    17341 atgaacgcgg ggacgcgctt gggtgaaatg aaggattcgg tcaaggccgt gcgcatggtg
    17401 gatccgaagg ggcgggtgat ggacattccg gccgccgaga tcccgttcag ttaccggaag
    17461 gcacatctgc cgcgcgggat cgtagcagca gtgtggctgc aattgaagcc cggcgcgcac
    17521 gatcagatcg aaaagaccgt gaaagagtat ttgcagtatc gcaagaacac ccagcccttg
    17581 acgctcccca gcgccggctg cgtgttcaag aatcctccgc aggattccgc cgggcgttta
    17641 gttgacgtcg cggggctcaa gggtgcgcgt gtcggggatg cgcaggtctc agagaagcac
    17701 gccaatttca tggtgaacct cgggcatgcg cgcgcggcgg atgtgctggc cttgatcagg
    17761 aaagtgcgcg cgggagtgaa gaaggcctcg ggcgtcacac tggaattgga attgaaggtg
    17821 gtcggtcagg cctgaggccg atcgatgaag ggagtgcatg tgacggaacg gaagccgctg
    17881 actcgttcga aaatcggggt gttgatgggg gggcagtcct cggaacgcga agtctccctc
    17941 aagacaggcg aagccgtgta ccggtcgctt gtgcgcagcg gttacgatgc ggtggcaatc
    18001 gacgtgggac cggggttgtc ccagaccttg caagaccagg cgatcgaggt ggcgttcctg
    18061 gcattacatg gaccaggcgg ggaagacggc gccatccagg gatttctgga aaccctcggt
    18121 attccctaca caggatccgg tgtgcgcgcc agcgccatcg gcatgcataa ggtggtgacc
    18181 aaaacagagc tggccgccca aggtattccg gtgccgcgcg gcacggtcgt gtggcgcggc
    18241 acagcgccca cgttgaatcg tatcctgacg agctgcaaat tgaagttgcc gatcgtcgtg
    18301 aaaccggcga gccaggggtc gaccatcggc gtcaccatcg ttcgccaacc ttcgcagtgg
    18361 aaagaggcac tgaaggtggc ccatcgatac gatccagaag cgatggtcga ggcgtttatc
    18421 cccgggcacg aagtcacgct gtcgttgtta gggactgctg atgggagcgt tgccggattg
    18481 cctgctgtgg agattgtcgc accggacgga ttttatgatt tttcggccaa gtacgagaag
    18541 ggccgcacgc agtatctttg cccggcgcca ctgcctgctg ctgtgagccg cgacatcaag
    18601 caattggcca tccgcacgta tcaggctttg ggctgcaacg gagcggcgcg ggtggatttt
    18661 cgaattacgc ccaagggcaa accctatgtg ttggaaatca acacggtccc gggaatgacg
    18721 gagacgagtc tgttgccaat ggcggcaggc aaagccggtc tgtcatacga tgcgttgacg
    18781 gagcagatcc tccaatccgc ccttcggcgg gctggaacgg gatcatttcg agccgtgagg
    18841 taacgatgcc gctcaggtgg cggaacacgc gaccggccaa actcaatccg cgggcgaatg
    18901 cccgttcgga atcgggcctg gaacgtcgtc gtggcggagc gacaggcggg tattggacac
    18961 ggcttgggcg aggcctgctg attaccttgc gagtcatcgc cacggtgacg gtcctggtcg
    19021 gtggctgttc gggactcttt gtgctggcgc gcgaaatagg gccgctgacg cgcgagtggt
    19081 ttctggtgcg ctccgtgtcg gtgaatggtc tgcatcacgt gaccagaaaa gaggtgatcg
    19141 gtcggctggc gctcaaaccg gataccgcgc tctattccat caatccgacg tggttggcgg
    19201 aacggatcaa gactcatccc tggatcaagg atgcgaccgt cgtgctgaag ccgttgcacg
    19261 aaatccatat cgacatcgtc gagcgggagc cggcggtggt cgtccgcacg ctggcggaaa
    19321 atttgttgac cgatgccgaa ggggtgttgc tggcgcgtct ggggtccagc gatgacccga
    19381 cgcttcccat cctgtcgggg gtcgatggca agcggttggc acaaggccgg gcggaggatc
    19441 gccgccccgt gcaggtaggg gcggccttgg cgcgcatggt ggggcagacc accggcggtc
    19501 ggccggacat caatgtgggg aatttgaata atctggtggt tgaggttcaa ggtgtcacgt
    19561 ttcagttcag tgaatcatcg atgaatcagc aatggtctcg gtttttgaaa atgcggcccg
    19621 cactgcgtga tgtggccttt gacggcgagg gcgccagagc gaatgaaatc gatcttcgct
    19681 ttgccgaccg cgtcattgtt cggggaaggg ggtgagccga gtgtctaagc gggatcatat
    19741 cctggtcggg ctcgatatcg gaacgaccaa gatttgcgcc attgtcgccg aagtgccgga
    19801 ggagggcccg ctgaacatca tcggcgtcgg ctcctgtcct tcgcgcgggc ttcgcaaggg
    19861 tgtcgtggtc aatatcgaga gtactgtgga atcgatcaag aaagcggtcg aggaagcgga
    19921 actcatggcg gccgtgcaga tcaattcggt ctacaccggc attgcgggca gccacatttc
    19981 gggagagaac ctcaagggcg tcgtggcgct caagaaacag gaagtcacgc gcgatgacat
    20041 cagtcgtgcc gtggagagcg caagaacgct ggcggtcatt ccccatgagc ggcgcattct
    20101 gcatgtgttg ccgcgcgagt tcatggtcga cgatcaggaa ggggtgcgcg agccgctggg
    20161 catgtcgggc aaccggctcg aggtcaatgt gcatgtcatc acgggggcgg tgacctcggc
    20221 ccagaatatc atcaagagcg tgaaccgggc aggcctcgac gtggttgaca tcatcctgca
    20281 gccgctggcc tccagcgaag cggtgttgag cgcggaggaa cgcgagctgg gcgtggccat
    20341 ggtggacttg ggcggcggca ctaccgacct cgccattttc ctcgatggga gcatccggca
    20401 cacggcggtc cttcccatcg gcggacagaa tcttaccaaa gatctcgcca ttggcttgtt
    20461 gacctcgcag accgacgcgg aaaagatcaa agtgcaacat ggcatcgccc gcaccgagct
    20521 ggtccacggg catcagatgg tcgaagtgcc ctcggtcggc gaccgtccgc cgcggcaatt
    20581 cacgcgtcgc gacatcgcgg aaattctcga acctcgcgtc gaagaaatgt tcgacctcgt
    20641 caagcgcgaa atcgtgcggg ccgggtatga aggcatgctc ggcgcgggcg ttgtgattac
    20701 gggtggaacc tcgctcttgg aaggcatgcc ggacgcggcg gagcgcgggc tgaatctgcc
    20761 ggcccgccgg ggcgcgccga caggtatcgg tggattacgg gatatcgtca gcaacccgat
    20821 gcatgcgacc ggcgtcgggc tgttactcca cgcgcgtcaa catgccgaca acttggaggc
    20881 tgccggcatt cgtcacggca aagggttggg taaagtgttt gatcgcatgc gatcgtggat
    20941 gttcgaattt ttctaactgt tgcggacggc ttcagaccat ccgcgccatc gtgaagggag
    21001 gtgccacaat gttttcattt caagaggagc cgcaatcgcc cgttcgcatc aaagtgatcg
    21061 gggtcggagg cgcagggtgc aacgccgtca atacgatgat caccggcgga ctgtgccggg
    21121 tcgactttgt ggcggcaaat actgacgtgc aggcgctcga acggtcgcag gcttcctata
    21181 aaattcaaat cggtccggag cgcacacgag gcctgggcgc gggtgccaag cctgaggtcg
    21241 gtcgcgatgc ggccctcgaa agcaaggatg agatccggga gagcctggtc ggggccgaca
    21301 tggtcttcgt cactgccggg atgggcggtg gcaccgggac cggcgccgcg ccgatcgtgg
    21361 cgagtattgc gcgtgaactg gggattctca ccgtcgccgt cgtgacgaaa ccgtttcaat
    21421 atgaagggca tcgccggatg agccacgccg aggaaggcat tcgcgatctc ggccggcatg
    21481 tcgacacgct gctgatcatc ccgaatcaac ggttgctggg catcgtggat aaggcgacgc
    21541 cgttgctgga tgcgttcaag gtcgcggacg atgtgttgcg ccaggccatt cagggcattg
    21601 ccgatgtcat tacaaccacg ggactcgtga acgtcgactt cgccgatgtg cggactatta
    21661 tggcccatac gggacgtgcg gtgatgggca tgggcatcgg ccgcggcgcc aaccgggcac
    21721 aagaggccgc acagaaagcc atttgcagcc cgttgctgga agaaggtagc gtggaagggg
    21781 ctcgcggggt gttgctcaat atcaccggtg ggccgaatat gtcgctgcat gaagtagaag
    21841 aggccgcgtc gatcgtgcaa catgcggcgg atgccgaagc gaatatcatc gtcggccagg
    21901 tgatcaatcc ggaaatcggc gacgatctga tcgtgacggt tattgcgacg ggattcgaac
    21961 gggaagagca accggccgct cgaccggctg ccgccgcgga gcgtcctgcc gcacggactc
    22021 ccaacgggcg tcctgcccag caggtgttga ccggcgtcca tgcgacagga tccgatcggc
    22081 ccatcaagga catcgatcgg ccgaccttcc tccggcgcat gggtgagacg cgggaagcgg
    22141 tggagcgaat cgcagttgtc ggcgacgatg agtgggatgt gcccaccttc ctgcgcaagc
    22201 aagccgattg aagatgagtc cgttccggaa gacgtaaggt cgcaggagtc gatggcgtca
    22261 gagttcatta cccttccctc gtttgcaacc catgccgacg gggcagaaca ctttttcggc
    22321 acccggctgt ccgccatacc ggtcaggccg ggacgtgcct ctgcgcacag aacggaacaa
    22381 caggatcaca gagcgcctgt gatcctttcc gtcaagcagg tgcatgggac cgatgccttg
    22441 gtcgtcgatc ggccggtcga gcagggggag ctgttcgagg gaggctggga tgcgctggtc
    22501 accaatcagc cgggactgat ggtcaccgtg cgaacggccg actgcgtacc cgtcctgttg
    22561 catgatcctg tgcgacaggt cgtggctgcc atccatgcgg gctggcgtgg cgctgtggca
    22621 ggcattgtgc cgaagactgt cgcgttgctt gccagccgat tcgggaccgt gcccaaagac
    22681 gtgcgcatgg ccatcgggcc ttccgccggt gcctgttgtt atgaggtcga cgaaccggtt
    22741 ctgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccct cgtgtcctca
    22861 cggtaacggg ggcggctagt cgtcatcctg aatctccgcg cctgcaggtg cccgggatcc
    22921 atcggagttg agcgaaagcg gcaatctgca aggcggcagg atggattcag agctgggcac
    22981 aattgccggt cgggttcgag cggtgttcga cgagattcac cgagccgcgg tccgtgcggg
    23041 acgggctccg gactcggtgc ggctgctggc ggcatccaaa accgtcaccg tggaacggtt
    23101 gcgggaggct gtggatgccg gtattcgaca tttgggtgaa aaccgcctgc aggaggcgct
    23161 cccgaaaatc gagacgctgg atcgggaagg ggtcgtctgg catttcatcg gcaccctgca
    23221 gcgacggaaa gtaaagtcgg tcgtcggtcg gttcgagacc attcactctg tcgacagcct
    23281 cgccctggcg gaagagatcg atcgtcaggc caaggcagcg gggttgaagc aacgggtgct
    23341 gctggaggtg aatctcggag gagaatccag caaaggcggt tttgcccctg cagcagtggc
    23401 ggcagccctt cccgctctga accaattgga gcagttggat attcgtggtc tcatggccat
    23461 tcctccgccc atgccgactg cggaggacgc gcgtccctac ttccgacagc ttcgcgaact
    23521 cgcgcaggca ttgacagcgc agggctgccg gaacattaat atgcaggaac tgtcgatggg
    23581 catgtcccat gactacgcgg tcgccattga agaaggcgcg acgtacgtca gagtcggcac
    23641 cgcaattttc ggggcccgga gtgagccgta tcatgatcac acgtaacatc gccgttcttg
    23701 gcggcgggca gatggctgag gcattgattg gtggtctgtt ggcctcgaag gtcagtgagc
    23761 cacagttcat ctgtgccaca gaccccgttt cggcccggcg ggatctcctc aagtcgcgtt
    23821 ttggcattca ggttggtgaa gataatgcca aggctgtcgc gggggcagat ctcgttctct
    23881 tggctgtgaa gcctcaggtg ttgcccgctg tgctccagga cgtgagttca gctctcagag
    23941 ggaaactcgt ggtttcgatt gctgccggtg tcacgacggc gtggattcag gagcgggtct
    24001 cggctgcgag aggcattgtg cgagcgatgc cgaatacgcc cgcgctggtg cgcgaagggg
    24061 tcacggcatt ggcctatgcg gctgacctcg ccgctgatga tgtggcggcg gtgcgggctc
    24121 tattcgaagc ggtgggctct gtggtctcgg tcgaggaacg gctgatggac gcggttactg
    24181 ggttgagcgg gagcggaccg gcctatgtgt tcgtggcgat cgaggcatta gcagacggcg
    24241 gagtgaaaat gggcttgccg cgtgcgaccg cacaattgct ggcggctcag acggtgctcg
    24301 gtgcagcacg gatggttctg gaacgagggc agcatccggc tcagctcaag gaccaggtgg
    24361 cctccccagg tggcacgacc atcgcagggt tgcatcaatt ggaagtcggc ggcat
//
LOCUS       DAOQEW010000037        23698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_515_length_23698_cov_7.034725, whole genome shotgun sequence.
ACCESSION   DAOQEW010000037 DAOQEW010000000
VERSION     DAOQEW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23698
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_515_length_23698_cov_7.034725"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PLS35_06130"
     CDS             complement(<1..1765)
                     /locus_tag="PLS35_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNI18269.1"
                     /translation="MNEEERWARVQPGIVLAELNKQLGRRNLLFGPDPSSGDMCKLGG
                     MVANNSSGPHTLIYGSVKDNVQALRVCLPSGSWLSAVPMGLDDPALAQLLTAHPTLKP
                     ILALVQRQRALIDSKKPTVSKNSCGYNLFGLADGLAQGLFDLPKLFVGSEGTLGIVSE
                     ATLRLVPKPQGTLTALIHFRRLEEVGEAVPHLLNLRPSALEVMDANTLNLIGRSAHGI
                     PADAAATLLAELDSSEGEADLRERADRMAAICRRYQLCGDLTIAYDKEQRDQLWKARK
                     ALYPTLYRFDPRKKPINFVDDVVVRAERISELIRYLETFFEGQHVPVAIFGHIGNGNA
                     HITPLLDVNDRQDFDKMVQAYHEIHQAVLSRFGGSICGEHGDGRVRAEYVRKMFGDEL
                     YQLFVEVKRTIDPANVMNPGIKISDAPFTDHIDYQRLSKSCATCAKCNSVCPVYDVFQ
                     SEDMSSRGWYEIVTAKDYSYLNSKRVVEACLNCKSCRTICPAGVDVSELILQRRAEHP
                     NQGSRWLFAMQSRLPIFEAILKLLAKTQRLWDRPGPRALLERLAAPLLKRIAPTAKLP
                     ADMVLPKLAPQHLRDRYPELTQ"
     assembly_gap    1816..1915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1971..2936
                     /locus_tag="PLS35_06135"
     CDS             1971..2936
                     /locus_tag="PLS35_06135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNI18270.1"
                     /translation="MAAPTLYISRLLPDPVMTAARRQFQLLNDPRDASPSWDNVKTGL
                     READAAICTLTDRVDASMLAEATRLKILANYAVGYNNIDLAAAKARGIVVTNTPDVLT
                     DSTADLTWALLLAVTRRVAEGDAYVRSGDWSGWAPTQMLGTDVSGKTLGIVGMGRIGQ
                     AVAQRATGFNMRILYTSRTPTALERVPSQWEPRTLPDLLKEADFVSVHVPLTPATHHL
                     IGARQLALMKPTAFLINTSRGPVVEEAALVDALLQRRLAGAGLDVFEHEPAFHPSLRE
                     LRQVVLLPHLGSATLATRVRMGMICLENIAAVVAGKPAPNQVHAA"
     gene            complement(2992..4953)
                     /locus_tag="PLS35_06140"
     CDS             complement(2992..4953)
                     /locus_tag="PLS35_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18271.1"
                     /translation="MAVDRRTVLQNAQLFASKGQYDAAIAEWRKLIADTPNDGTVFNS
                     IGDLQLKRNATAEAVTAFLQAASAFRSEGSVLKAIAAYKKILKVDPARIEIYRHLGDL
                     NAERGLLSSAVQDYLTLGKHYFKDGRNKEALEIYRKIVSQDPSNLDAQLRVAELCVQE
                     GLVDEAVRMYLQLGREQSAAQQYEQAKAMYTAVLRLDPENAEAKEITSMIETGGSVTP
                     SAARPAKPATTAAKPGDASDLMAEATRRIEEGQYAGAEAMLTQLLSREPGNPEICQLL
                     ARMHLLQGNLVVALGEYRFLAGAALRAQDYGVAESLIADYLKVEPASVPMLELLGELY
                     QEKGDAATAAQHFGRAVEILLEHPEPGMPTLPAELFEKVQALAPGSPIARKLAPAFDP
                     NAGSMPEPEPIAPVMEVVLTPAPEPVVEPAPELEPTPVIMAAAPPAEVPFRFVGDPAP
                     PLEAAVVKPPNVEPVVEPVVVQAAPPPEVPPAPVVEIEPVIAAAPPPQAPPPVAPPAP
                     VVEPIDAETRYALGMAYKDMGLCEEAKEEFILSMKDSGFFVDSCLMMALCCKEQSRPD
                     QAIQLLEKLISDSRCRGGNAQLVRYELALLYEMTGARDRALTMYQSIPSFHDVPRRVE
                     ALRMQSTNGAAHPELPASTPTSPLPIAGH"
     gene            complement(5133..5351)
                     /locus_tag="PLS35_06145"
     CDS             complement(5133..5351)
                     /locus_tag="PLS35_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNI18272.1"
                     /translation="MKTKGTVKWFNDRKGFGFIRLDGGDDVFVHYSALQGDGFKTLKE
                     GENVEFDIVQGAKGPQAANVLKDLAPAN"
     gene            5664..6749
                     /locus_tag="PLS35_06150"
     CDS             5664..6749
                     /locus_tag="PLS35_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNI18273.1"
                     /translation="MLHTILDRYIFRELLSPFCISLGALCFVMLTKELLRLVELLVTK
                     GIGFLSVLKVFAHLLPSFLVLTLPIAGIIASITAFGRLSLDKELVAMRAAGFSLLRLS
                     QPVFLFAALVCGLTLMMAQYGQPWSNVNLKKVALNLLRDELVLELDRGVFNEAIPKMV
                     IYVPDAQEGQDNRGIFVADERNPADPRIIVAQQYQVMTDSASSQVALRLMNGVIHSRP
                     QNPEEYQKISFTAYDLKLSINASLYGAEERTPMDVIRAKLESSNWTDTNALRRLMEYY
                     KDLAFPAASLVFCILGVPVGIVSKRSGSIGGFAVGVLVVIAYYVINVACEFLVTTLWI
                     SPFAGAWLPNVIFTIVTILWFYRVSRQ"
     gene            6754..7845
                     /gene="lptG"
                     /locus_tag="PLS35_06155"
     CDS             6754..7845
                     /gene="lptG"
                     /locus_tag="PLS35_06155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNI18274.1"
                     /translation="MNILFRYMLREYVKIFGMCFSGLMTIYLVIDFFEKVRRFLRYDA
                     HALDVLAYFVLKMPAISYQIAPLAVLMATLLTIGLLSRSHEITAMRSCGISLYWITSP
                     FIFLGTVLAMVLFLFSSTVIPLALAKADQIKTTQIEKRVTPVSLKAAQPWVSLGGQTL
                     LNIDTIDPGGAVLRNIRLYRLSPTFQLEEIAEAKSAVYSSQGWALHDGMTRSFRQDGT
                     VLVSSFQTQPLALPHIPDDFTTWAELDSETMTLREIRAYVDRLHLGGTMLPRLLTDYY
                     GRIAFPCVTLIMVIVGIALSLRRTGVRGSGMAMGIGQAMAVGFFYWSTHSVAIALGRG
                     GALSPMLAGWMANMVFLTFGLYLLMKVRY"
     gene            8098..9354
                     /gene="fabF"
                     /locus_tag="PLS35_06160"
     CDS             8098..9354
                     /gene="fabF"
                     /locus_tag="PLS35_06160"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNI18275.1"
                     /translation="MSTRVVITGLGVVSPIGIGVPQFWKAALEGRSGISAIPSFDPFP
                     LEGYRSRIAGRIVDFSPDQYLPAGQGDRVDRYAQFALVAAKEALADADFKMERENPHR
                     VGVIVGAGMGGMVMGEREITQLYEQKRPHRVHPNFIPVITLNSASGIVAMAYGAKGPN
                     LTISTACSSSAHALGQAMHAIRAGTADAVIVVGADASITPLVFAGFCSLRALSTKYND
                     APEKASRPFDRGRDGFVMGEGAGALILESLAHAKKRKARMYAEVAGYAATSEAHHMVI
                     PREDGEEVATTMRLALKDAGVTPAQVDYINAHATSTTVGDAVEVKAIRLVFKSRADKL
                     AVNATKSLVGHTLGAAGSLAGVVSALTLTSGQIHPTLNLDDPDPACALAGLSKDQQTR
                     KAKVALINAFGFGSNNAAVVLKAVSS"
     gene            9417..9776
                     /locus_tag="PLS35_06165"
     CDS             9417..9776
                     /locus_tag="PLS35_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNI18276.1"
                     /translation="MATTTDVSSKIIQALAEYLKRDASSITPTHHLREDLGLDSMAVI
                     EMLYRIEEVFNLQIPDQDLVGLTTVGHVIAYVEGRVAPASTKTAAKGAAKPSAKTPAK
                     PAAKKPAAKPSPESKKA"
     gene            9781..10863
                     /gene="lpxD"
                     /locus_tag="PLS35_06170"
     CDS             9781..10863
                     /gene="lpxD"
                     /locus_tag="PLS35_06170"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNI18277.1"
                     /translation="MSTAPRRTPLTLRELHDYVGGELIGSPDATVTAVASLEQAGPGD
                     LAFVTSERHLKAPQTATFGALLVGRRIADCPSPQIVVENPAYAFARAAQQFFARPARV
                     RGIAEGLTRGDGVTIGTDVSIWPGVTLGDRVTIGARVTLYPGVFIGDDTSIGDDSLLY
                     PNVVVREGCRIGARVIIHSGTVIGSDGFGYVQYQGRHQKIPQLGGVLIEDDVELGANV
                     SVDRATFGDTVIKRGTKIDNLVQIAHNVVVGEHNILVAQVGIAGSTTLGRYVMVGGQA
                     GLADHLQIGDQVMIAAKSGVTRSLEPNQIVSGAPVMAHATFLKAQAVIPQLPELRNRV
                     RELEERLATLEQAAKPAAKTRKPRRK"
     gene            complement(10881..11405)
                     /locus_tag="PLS35_06175"
     CDS             complement(10881..11405)
                     /locus_tag="PLS35_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18278.1"
                     /translation="MPKLVSVKQQEGHDARATAYIKAYMLFPAGILGLISMLGGVGGL
                     AYQLIATDTYSWTTFVESSGLLLLGGLLGWVQTTYHRWILANRPEVFAERMRQPALTK
                     SGKPKRESGKNQESSSGSPWAPGAYMVGLAVLLAGSMLSMLYGAVHPIPACFLPWAGF
                     FWAKLFFWKTLLKP"
     gene            complement(11658..13745)
                     /locus_tag="PLS35_06180"
     CDS             complement(11658..13745)
                     /locus_tag="PLS35_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNI18279.1"
                     /translation="MEQNHYRHAEEILAKVAASVQRQEDVDLTELTHLADSIVESLQG
                     SDQLVVEALSSPSGPPLVTNLINVGILATKVGIGLGYYGVELHRLAVAGLLHDIGIFA
                     VPQQLLTKAGRLTAEERTLVEQHPRLGSEVIRRVGTEYGWLAEVVLQAHERGKGQGYP
                     HRLKGREINELAQIIGLVDIFDALVSPRPYRRRLLPHEAVRELLTTERTAFPREIMKA
                     LVEQLSVYPLGTRVRLSSGQEGVVVRITARYPSRPVLRITSTETAAPSEPPHLLDLSL
                     LPHVSVVDTVEPPALDRVTFEAAGAAPAASAAPTNVSDQFAALLESLDAIAGVIQAAV
                     DQPAVARGPAQVPAEAETAEPLIPVRREILGLFVLEAREWLNQIQTALERLDATSEQA
                     RRAQLASILWQSVNNLARSAATVGLTAVETMATRLFPLLQAAARHERAVAAHHVASLR
                     EGLLEISKTVQELRPIEEHPAPAEETVEREQPILEQPARTELPESQASSEPASEIATP
                     RYAPPRPMPASSILDALRQLHVARGRSLEPVRDVLETVIQRAERELAQGAPVVDARAI
                     GRLLQELDELDERFLNHMQARVPSVVSTLGRIARASEERVLPPQALEPIFEEIDVLFE
                     ASEKVSAENISVFLQGLRTFLRVTAQHKPAVIRERLAAVEERLATLIPLAQQWVDVGR
                     VERAAIFDILPMA"
     gene            complement(13928..14875)
                     /locus_tag="PLS35_06185"
     CDS             complement(13928..14875)
                     /locus_tag="PLS35_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18280.1"
                     /translation="MSTEPEQTQDDFQKELIELFGQEAQEWLVQIHAALVELESLPDS
                     DRHSQLIDAVVRGITSLGGSAATVSLPDVERATFALLPFIETIKDRTTATKQDFSTVR
                     EHFRTVIASVKVATGITLDIDPLPEAAPAPEPVLEFLPLLNALHQLQDEQAAQGSMSR
                     SLIPQVLQRLEHEARIGAGQITAAQFQELLLTIHNTDAQYLGSLRQDLPGIALHLSRL
                     RAGGVAVLTSDAGFDAPIQMIASLHEIAKQTNATPLVTFLTGLQNFLTLVVQRRIDIA
                     AHRLQSVETRILAVASMVEEWIADGQKEFDMMSRLAPST"
     gene            complement(14932..16407)
                     /locus_tag="PLS35_06190"
     CDS             complement(14932..16407)
                     /locus_tag="PLS35_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18281.1"
                     /translation="MPKILIADDSIAVRKVAERLLTEAGMGVTLAANGSEALAMLAKD
                     RPDLIVSDVIMPDKSGYEVCAFIRGQANLADLPVLLISGIVNDEVSKQAESCKADGVL
                     KKPFQGSSLKDRVLDLLTKRPHKPAPVAEPIRIPIAEPPVMAEVAPAPVAPAQEELPS
                     IPLVQREWQQPAEPVAIVLDQTPAGPVGIEESRAGSISIHQAAEPSPVMQADDRHQAL
                     LADRDAKIAELEAQLGAERRHSQEEREQHQLTVSEQREQMADVSSRMQALEQDLAKER
                     AQRESMTQQLEDLSRQAHRLGEVEAALLEERHRADQLAQQAAESARLGARIVELEAHL
                     EAEREAANQLVNQITSLEHVEDRMRELERVLASEREEAMALRNERAHLEKSAAMVPVL
                     EEAGEHARAQVAELERTLTAEREAAAALLAQYKQMEEKAQLVPDLEQELSAERDHSMQ
                     LTKQVADAESMAEQSTRRFEDLARKLGEIAGLASQLGNAKR"
     gene            complement(16436..16912)
                     /locus_tag="PLS35_06195"
     CDS             complement(16436..16912)
                     /locus_tag="PLS35_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNI18282.1"
                     /translation="MLRSQLRRHPHTTASGAAHARVQNMVVFSVAGKRLAARTEEIGG
                     VLPWPGTTAVPSDTPLVTSLVRHEESCLPVFDLAAKFNRTVREEESLCLIVKHVDGPL
                     AICIDSQVPSLHVVPRSGVQYQSGLDPDIAGSCVAGDEQLPLINLATLGVASQRPA"
     gene            complement(16928..17500)
                     /locus_tag="PLS35_06200"
     CDS             complement(16928..17500)
                     /locus_tag="PLS35_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18283.1"
                     /translation="MGLRGQRAVTISSGQAVRFLVTLIGGSSVAFPAQWVRGIVMPAS
                     AGPNGLVTWAGVEYERTDLSARLKVVSKGVSSDTRLILYGNDERSRSFAVDKVVGLLD
                     VERALIHPLPPQFRGKERERLLGFFVGASFVALIANPLWVLELPLRAQTLEMFAIRFA
                     ESKAGESDSRLHVPMVGVDEAMSMSTSPVS"
     gene            complement(17548..20928)
                     /locus_tag="PLS35_06205"
     CDS             complement(17548..20928)
                     /locus_tag="PLS35_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNI18284.1"
                     /translation="MSADFDRDQLLDIFVAEAGDDMERFWKALHPEGKGRPEPAEVAE
                     FHAVGHKLKGAALLYGFPGLGQLGALLEETLEHVQEMSPSQWPAAFEVIRDIVASFRH
                     QVTRIGRGEGEDASLVEGFVQRCAQFLPAASADDSSQCLPIATEPADDYLLPVMDEEV
                     LSYFAPEAEEYLNTIQTLLQRLELDLSDAETIHQLYRVAHTLKGSAYTVGFQVVGDVA
                     FPIETCMIAVREGAAVIGPQWIATIRHAVDVMRSLMARDAQQLPRLRQDVPCMTTMLR
                     ELEQGMTQAPQVDAPAASSSSVAAEEALPPAAKPLNPAAQLSEPNHPAELSEAYLIPM
                     LDAEVISYFAPEAQEYLESLETDLLRVDKDAANPDIIHQLFRTAHTLKGSAYTVGFQS
                     IGDLTHHIEDFMGAVREGRLKFLPGHTDVLLRAIDVIRALMRRDPSLVTRTRQRFAAS
                     LQELKGLGEAAPAGVHEPVMSSQTEAVIALDVQEAEQAEPVNAAEGKAGEEREVIRVS
                     RDRLERLLNLVGELVIDRGRLEQRLRTLDQLATQVLANKNRLTDAVRTFEDKHTFSFQ
                     PSPTTQGEAMPQGFPGVSDFGSLEFDKYDDFNILARRISEVTADISESMSQLSGSIHR
                     AQDDMGSLQQLTLGMRDEIARARMVPIGTPFTRFRRAAREMARATGKDVNLVTSGEHT
                     EIDTGVVERLVDPLVHLVRNAVYHGIEPAAVRRVQGKPETGTIYLHAAHRGNSVLIEV
                     EDDGAGLDIAKIKAKAVKLGLVRQDVAETLPESEVIKFIFLPGFSTADAVGDQAGRGV
                     GMDVVKRVIETMNGHIEVESVRGQGTKFTMHLPLTLLIATALLVRVGKDRYAIPLPSV
                     REVTMSTASTLQHMGDRQVVQIGDEAIEVYPLGSLIRREAGITDGVSPVVIVRTSTGA
                     LGCAVDELLGRQEIVIKSLGGLKPYERSVFGGATIDPEGRVVLVLDVSRLTTREYQET
                     MSIAQDSSVAAIEDATGHSEPKASAPSALPLLLIDDSLSIRKFVGRMLEAAGYTVETA
                     TDGEEGCRKAMVQAYQLIITDLEMPKLNGYEVIQALRARPQTHATPILVMTTRAGEKH
                     RQMAVSVGASGYIAKPVEERALIQEVRKWTGVEARAEK"
     gene            complement(20981..22855)
                     /locus_tag="PLS35_06210"
     CDS             complement(20981..22855)
                     /locus_tag="PLS35_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNI18285.1"
                     /translation="MIGQGVTNRFQDMKTRSKLLLSFGLVSFIIMIMASVGVFTLRQL
                     SAQSQTVYVDYTVPLAEFAQMGTALTKHHQILLDVASATKQSDFAQEITKLPPLKAEI
                     EKAINNYKSTNLRVSRSGRDEMKDLTLFEPALKKYFQEADGALSAMADSFDRNTLSPA
                     QAEQMRALGILALTVNLTPSFENAVRRHNEQVSSIESVAKDLNDDAQSLASNGTFILV
                     AGGLVAVALGLFVGYLLATFLSRNISHIANVATQAAGGNLQARAKIESHDELGQMATA
                     FNSMLDRITALVSTEEERDLMQKRLMQFLVLVSEVGKGDLTRRGEVTADMFGNLADGF
                     NLMIARFGQLLKQVREAADRVNKSAGTLRDSAGQMSGTARAQAEESVRTLGAVEQLAA
                     GMRQVASTAGASSDSAKQVLSATERGNVAVQETVRDMQSIRSAVQRMSKQVKGLGDRS
                     LEISQIVSTIRDIANQTNLLALNAAIEAAGAGEAGARFAVVADQVRKLAESSTQATRE
                     IADLVKVIQTETQDAVVAMEHETQAVEAGSASALRTGDVFAEISDIAKRSSELAQNIA
                     SAASEQTTSTEKVGRAIKEFTGGAVATQKQTDSTRLTIEDMAKLAEGLNSSVAQFKLA
                     "
     gene            complement(22951..23496)
                     /locus_tag="PLS35_06215"
     CDS             complement(22951..23496)
                     /locus_tag="PLS35_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNI18286.1"
                     /translation="MSDVPIIQPQASATSPAQGSSGSSRTSAASTEGALRMCVLTLGG
                     ELFAIDLRHVSEVFEVEAVTAVPSMPSYLTGVTNLRGTVITLVDLRGSLGLSVNNTAL
                     PFAVVIRHGARQVGVLVDHVPEIHTVPREHLLPAMQAGPAGARPCVSAVLRVDHRLGG
                     VLEVPQVFAQVDGGSAALSVV"
     gene            complement(23500..>23698)
                     /locus_tag="PLS35_06220"
     CDS             complement(23500..>23698)
                     /locus_tag="PLS35_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18287.1"
                     /translation="PGRNGFQACRDLKSDDRFKGIPIVLCTSKGQESDKFWGQQQGAN
                     GYVVKPFKAEDLVAAVKRALN"
BASE COUNT         4573 a         7294 c         6910 g         4821 t
ORIGIN      
        1 gctgcgtcag ctcgggatac cggtcgcgta ggtgctgcgg cgcgagcttc ggcagcacca
       61 tgtcggccgg aagtttggcc gtgggggcaa tccgcttcaa cagaggagcc gccagccgtt
      121 ccagcaaggc gcgagggccg gggcgatccc agagacgctg cgtcttcgcc agaagcttaa
      181 gtatcgcttc aaatatcggc aacctggact gcatcgcaaa cagccatcga ctgccttggt
      241 tcgggtgttc ggcacgccgc tggagaatca gctccgagac atcgacgcct gccggacaaa
      301 tcgtgcggca ggatttgcag ttcaagcagg cctccaccac ccgcttcgag ttgagatagg
      361 agtagtcctt ggccgtgacg atttcgtacc agccgcgcga gctcatgtct tccgactgga
      421 acacatcgta gaccgggcag acggaattac acttggcgca ggtcgcgcag gacttggaca
      481 gtcgctggta gtcgatgtgg tcggtgaacg gcgcgtcact gatcttgatg ccggggttca
      541 tcacgttcgc cggatcgatg gtccgcttca cctccacgaa cagttgatac agctcgtcgc
      601 cgaacatctt gcggacatat tcggcacgca cccgcccatc accatgctca ccgcagatcg
      661 agccgccgaa gcgggacaac acggcctgat gaatctcatg gtaggcctgc accatcttgt
      721 cgaaatcctg ccggtcgttc acatcgagca gcggcgtgat gtgggcgttg ccgttgccga
      781 tatggccgaa aatcgccacg ggcacatgct gcccttcaaa gaaggtttcg aggtagcgga
      841 tcagttcgct gatgcgttcg gcgcggacga ccacgtcatc cacgaagttg atcggcttct
      901 tgcgcggatc aaaccggtac agggttgggt agagggcctt cctggctttc cacagctggt
      961 cgcgctgctc cttgtcgtag gcgatggtga ggtcgccgca tagctggtac cggcggcaga
     1021 tcgcggccat gcggtcggcc cgttcgcgca ggtcggcctc gccttcgctg ctgtcgagct
     1081 cggccagcag ggtcgccgcg gcatcggcag gaatgccatg cgcagaccgg ccgatcaggt
     1141 tcagcgtgtt cgcgtccatg acctccagcg cgctcgggcg gagattcagc agatgcggta
     1201 ccgcctcccc gacttcttcc aaacggcgaa agtgaatcaa cgcggtgagc gtgccctgcg
     1261 gcttgggcac gagcctgagc gtcgcctcgc tgacgatacc caacgtgcct tcgctgccca
     1321 cgaacagttt cggaagatcg aagaggccct gagcaagccc atccgccaag ccgaacagat
     1381 tgtagccgca actgttcttg ctgacggtcg gctttttact gtcgatcaag gcgcgctggc
     1441 gctgcacgag ggcaaggatc ggcttcagcg tgggatgcgc cgtcagcaac tgtgcgagcg
     1501 ccggatcgtc caatcccatc ggcaccgcag aaagccagga ccccgacggc aggcagaccc
     1561 gcaacgcctg cacattgtct ttcactgagc cgtagataag tgtgtggggg ccagaggaat
     1621 tgttggcgac catgccgccg agtttgcaca tgtcgccgct ggacggatcc gggccgaaca
     1681 ggagattccg tcgtcccagt tgcttgttta actccgccag gacgatgccc ggctgcaccc
     1741 gcgcccaccg ttcctcctcg ttcagttcaa gaatgcggtt cagcttggag acgtcgagga
     1801 tgatgcccga gccgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggttg
     1921 ttgagtcccg cgcgatccgc taagatgctc gtcagtccgg cgaggcgttc atggctgccc
     1981 ctaccttata tatctcccgt ctgctcccgg atccggtgat gacggccgcc cggcggcaat
     2041 tccaactgct gaacgatcct cgtgatgcct cgccctcttg ggacaatgtg aaaacaggcc
     2101 tacgtgaggc cgacgccgcg atctgcacgc tcaccgaccg cgtggatgcc tccatgctgg
     2161 ccgaggcgac gcgtctcaaa atcctggcga attatgccgt gggctacaac aacatcgatc
     2221 tcgctgccgc gaaggcgcgc ggcattgtgg tgaccaacac accggatgtg ttgaccgact
     2281 ctacggcgga tctgacctgg gcgttacttc tggcggtgac gcgacgggtg gcggagggcg
     2341 atgcctacgt ccgatccgga gattggtccg gttgggcgcc gacgcagatg ctgggcacgg
     2401 acgtatcggg aaaaaccttg ggcatcgtcg ggatggggcg catcgggcag gccgtggcgc
     2461 agcgggccac cggtttcaac atgcgtatcc tctatacctc acgcaccccg acggctctcg
     2521 aacgagtgcc gtctcaatgg gaaccccgca ccctccccga tctgctgaag gaagccgatt
     2581 ttgtgagcgt gcatgtgccg ctgaccccgg ccacccatca cctgatcggt gcgcgccaac
     2641 tcgcgctgat gaaaccgacc gcgttcctga tcaatacatc acgtgggccg gtcgtcgagg
     2701 aggccgcgct ggttgatgcg ctccttcagc gcaggctggc cggcgcgggg ctcgatgtct
     2761 tcgaacatga accggccttt catccgagcc tgcgcgaact caggcaggtc gtgctgctgc
     2821 cccatttggg ttcggccaca ctggccacca gggtccgcat gggcatgatc tgcctggaaa
     2881 acatcgcggc agtggttgcg ggaaagccgg cgccgaatca ggtccacgcc gcctaacaca
     2941 gctccacgcc gtgacgctcg agacgagagc aagactgtag aaggactggc cttagtggcc
     3001 cgcgatgggc agaggactgg tcggagtgga tgcgggaagt tccggatgcg cggccccgtt
     3061 ggtcgactgc atccgcagcg cttcaacccg gcgaggcacg tcgtggaacg acggaatgga
     3121 ctgatacatg gtgagcgccc gatcgcgagc gcccgtcatc tcgtagagta acgcgagttc
     3181 gtaccggacc aattgcgcgt tgcctccgcg gcagcgcgag tcagagatca gtttctccaa
     3241 caactgaatg gcctgatccg gacggctctg ctccttgcag cagagggcca tcatcaggca
     3301 ggagtccaca aagaatccgg aatccttcat cgagaggatg aactcttcct tcgcttcctc
     3361 gcagagcccc atgtctttgt aggccatgcc cagggcataa cgcgtttccg catctatcgg
     3421 ctccacgacg ggtgcaggcg gtgccaccgg cggtggcgcc tggggaggag gcgcggcggc
     3481 aatgaccggt tcaatttcga cgacaggtgc gggaggcacc tcaggcggcg gagcagcctg
     3541 tacgacgacc ggctcgacca cgggttcgac attcggcggt ttcacgactg cagcttcgag
     3601 cggcggcgca gggtcaccga caaatctaaa cggcacctcg gcgggtggtg ctgcggccat
     3661 gatcaccggc gtcggttcca attctggcgc cggctcgaca acaggctcag gcgccggcgt
     3721 taggacgact tccatcaccg gcgcaatggg ttccggttcc ggcatcgacc cggcattagg
     3781 atcgaacgca ggcgcgagct tccgggctat cgggctccct ggcgccaggg cctgcacttt
     3841 ttcgaagagc tcagccggca aggtcggcat gcccggctca ggatgctcca acaaaatctc
     3901 cacggcccgg ccgaaatgct gcgccgccgt ggcagcatca cccttctcct gatagagctc
     3961 gcccagtagt tccaacatgg ggacggaggc cggttccact ttgagatagt ctgcaatcaa
     4021 cgactcggcc acgccatagt cctgcgcccg cagggccgca ccggccagaa agcgatattc
     4081 gcccagggcc acgaccagat taccctgtaa gagatgcatc cgggccagta attggcagat
     4141 ctccggattg ccgggttcgc ggctcagcaa ttgggtcaac atggcctcgg cgccggcata
     4201 ctgcccttct tcgatacggc gcgtggcttc agccatgaga tcgctcgcat cgccgggctt
     4261 ggctgcggtg gtagccggtt tggccgggcg tgccgcactg ggcgtcacac tgccgccggt
     4321 ttcgatcatc gacgtgattt ctttggcctc ggcattctcc ggatccaggc gcagcaccgc
     4381 cgtatacatc gctttcgcct gctcgtactg ctgcgcggcc gactgctcgc ggccaagttg
     4441 cagatacatc cgcaccgcct cgtcgacgag cccttcctgc acacagagct ccgcgacgcg
     4501 taactgggcg tccagattag acggatcctg ggagacgatc ttgcggtaaa tctcgagcgc
     4561 ttctttgttc cggccgtcct tgaagtaatg tttgcccagc gtgagatagt cctgtacggc
     4621 gctgctcagc agcccccgtt cggcattcag atcaccgaga tggcgataga tttcgatccg
     4681 ggccggatcg accttgagta ttttcttata ggcggcgata gccttgagga cggaaccttc
     4741 ggagcggaat gcgctggcgg cttgcagaaa cgccgtgacg gcctcggcgg tggcgttgcg
     4801 cttgagttga agatcgccga tggagttaaa caccgtgccg tcgttaggcg tgtcggcgat
     4861 cagtttccgc cattcggcaa tggcggcatc gtactgccct ttggacgcaa agagctgcgc
     4921 gttctgtagc actgtacggc ggtcgacagc caaggagacc cctcacccga cgttgagagt
     4981 ccacgctaac agtcaaccta tttattgtca aaacaaatgg ataaaagaga gacaccgcac
     5041 acgcggcgtc cggagcgggg attctctgaa ggaaaccggc ggcccctcga tgaggcagag
     5101 gggccgcgga ttacgtatga ctccggccga aattagttgg ccggcgcgag atccttcaac
     5161 acgttggccg cctgcggtcc cttggcgccc tgaacgatgt cgaactccac gttctcgccc
     5221 tctttcaagg tcttgaaccc gtcgccctgc agcgcagagt aatgcacgaa gacgtcgtct
     5281 ccgccatcga gccggataaa tccgaatccc ttacgatcgt tgaaccactt cacggtgccc
     5341 ttagtcttca tacggtcctc cttttctcaa cgcacggaca taatgagcgc atcagctcgt
     5401 ctgctgagtc gcatgacagg aaatgatgac gataaaaaag gagaaacaac agcgacgagc
     5461 gcggcggctt ttgcgggggc gttcagacaa tattcaaccc gaaccatctt aacctgctga
     5521 aaagtgcgca gcgatgtacc atggggtgaa atgcttgtca agcggatctt tatggccctg
     5581 gcctatctga tctgtttaca aggcacgcag actcttctat agtgcagcat ccaattctcc
     5641 gatgtaggac cccgaccgct ctcgtgctgc acaccatcct cgatcgttac atcttccgcg
     5701 agttgctttc gccgttttgc atcagcttag gcgcgctgtg cttcgtgatg ctcacgaagg
     5761 aactgctccg cctggtggag ctgctggtga cgaagggcat cggctttctg tcggtgttaa
     5821 aagtcttcgc ccacttactc ccctcgttcc tcgtgctgac cctgcccatc gccggcatca
     5881 ttgcctcgat tacggccttc ggccggctct cgctggataa agaactcgtc gccatgcgcg
     5941 cggccggctt cagcctgttg cggctctccc agccggtgtt tttgtttgcg gcgctcgtct
     6001 gcggcctcac gctcatgatg gcgcaatacg ggcagccgtg gagcaatgtc aatctcaaga
     6061 aagtcgcgct gaacctgtta cgcgacgaac tggtgttgga gctcgaccgc ggcgtgttca
     6121 acgaagcgat ccctaagatg gtgatctacg taccggatgc tcaggagggg caggacaacc
     6181 ggggcatttt cgtggcggat gaacggaacc cggctgatcc gcgcatcatc gtggcgcagc
     6241 agtaccaggt gatgaccgac tcggcaagca gccaggtggc gctgcgcctc atgaacggcg
     6301 tcatccacag ccgtccgcaa aaccccgagg agtatcagaa gatttcgttt acggcctacg
     6361 atctcaaatt gagcatcaac gccagcctgt acggagccga agagcgcacg ccgatggacg
     6421 tgattcgcgc gaaactggaa agttccaact ggacggacac caatgccttg cggcggctca
     6481 tggaatatta taaagacctg gcgtttccgg ccgcgtcgct ggtgttctgc atcctgggcg
     6541 tgccggtcgg gatcgtgtcg aaacgctccg gcagcatcgg cgggtttgcc gtcggcgtgc
     6601 tcgtcgtcat cgcctactac gtcatcaatg tcgcctgcga gtttctcgtc accacgctgt
     6661 ggatctcccc gttcgccggc gcatggttac ccaatgtcat cttcacgatc gtgacgattt
     6721 tgtggtttta tcgcgtgagc cggcagtaac cccatgaaca ttctttttcg atacatgctg
     6781 cgcgagtacg tgaagatttt cggcatgtgc ttttcggggc tcatgacgat ttatctcgtc
     6841 atcgactttt ttgaaaaggt gcgccgcttc ctccggtatg acgcgcatgc gctcgatgtg
     6901 ctggcctact tcgtcctgaa gatgccggcc atctcctatc agattgctcc gctggcggtc
     6961 ttgatggcga ccctcttgac catcggcctg ctgtcgcgca gccacgaaat tacggccatg
     7021 cgcagctgcg gcatcagcct gtactggatc acctcgccgt ttatttttct cggaacggtc
     7081 ctggcgatgg tgttgttcct gttcagttcc accgtcattc ccctggccct ggcgaaggcc
     7141 gaccagatca agacgacaca gatcgagaag cgcgtcacgc ccgtctcctt gaaggcggcg
     7201 cagccatggg tcagtctggg gggacagacc ctgttgaaca tcgacacgat cgatcccgga
     7261 ggcgcggtgt tgcgcaacat ccggctctat cgcttgagtc cgacctttca attggaggaa
     7321 atcgcggaag ccaagagtgc ggtctattcc tcgcagggat gggccttgca tgacggcatg
     7381 acgcgttcat tccgccagga cggcacggtg ctggtgagca gctttcagac gcaaccgctg
     7441 gcgctgcccc acattcccga cgactttacc acctgggccg aactggactc ggaaacgatg
     7501 accttgcgcg agattcgcgc ctacgtcgat cgcctccatc tgggcggcac catgctcccc
     7561 cgcttgttga cggattatta tggccggatc gccttcccct gcgtgacgct gatcatggtg
     7621 atcgtcggca ttgccttgag ccttcgccgc acgggtgtcc gcggaagcgg catggcgatg
     7681 ggcatcgggc aagcgatggc ggtcgggttc ttctattggt ccacgcactc cgtggccatt
     7741 gcgctggggc gcggcggtgc gttgtctccg atgctggccg gatggatggc gaatatggtg
     7801 tttctgacgt tcggtttgta tctgctcatg aaggtgcgct actaaaagga tgttgaacgt
     7861 gtccgccagc gtcgttctcg gatcatacga accatcgatc taattggctg gaacgcctgc
     7921 gggccttcac gtgctgcagt cttgctggcg ccaatttgag cctcctgtgc ggtggttcca
     7981 ccacgatacc tacggagatc tcgagcggat gtctgcgtac agcattgact gtgacgcccc
     8041 cgtgcggtaa gtagacaggc cgcacacctc accatcctgc ggccatgaaa gggttttatg
     8101 tcgacgaggg tcgtcattac agggctggga gtcgtctccc ccatcggcat cggcgtgccg
     8161 caattctgga aagcggcact ggaaggccga tcggggattt ccgccattcc ttccttcgat
     8221 ccctttcccc ttgaaggcta tcggtcgcgc atcgcggggc gcatcgtcga cttctccccg
     8281 gaccagtatc tccctgccgg acagggcgat cgtgtggatc gatatgcgca atttgcgctc
     8341 gtggccgcta aggaagcgct cgctgatgcg gacttcaaga tggaacgtga aaacccgcac
     8401 cgggtgggtg tgatcgtcgg agccggcatg ggcgggatgg tgatgggcga acgtgagatc
     8461 acacaactct atgaacagaa gcgcccgcat cgggtgcatc cgaactttat cccggtgatc
     8521 acactgaatt ccgcctccgg catcgtggcc atggcctatg gggcaaaagg tccgaacctc
     8581 acgatttcca ctgcctgctc atcgagtgcc catgcgttgg gacaggccat gcacgccatt
     8641 cgcgccggca cggccgacgc tgtcatcgtc gtcggggccg atgccagcat cacgcccctc
     8701 gtcttcgcag gattttgctc gcttcgagcc ctgtccacga agtataacga tgccccggag
     8761 aaagcctcgc gcccctttga ccgtggacgg gacgggttcg tgatggggga aggcgccggc
     8821 gccctgatcc tcgaatcatt ggcccatgcg aagaaacgca aggcccggat gtacgcggaa
     8881 gtagccggat atgccgccac gagcgaggcc catcacatgg tcatcccccg ggaggacggc
     8941 gaggaggtcg ccacgaccat gcggctggcc ctcaaggatg cgggcgtgac gccggcgcag
     9001 gtggactata tcaacgcgca cgccacgtcg accacggtcg gcgacgccgt ggaggttaag
     9061 gccattcgct tagtattcaa gtcacgcgcg gataaactcg ccgtgaacgc cacgaagtct
     9121 ctggtgggcc acacactcgg cgcggccggg tcgctggccg gagtggtctc cgccctcacg
     9181 ctcacgagcg ggcagattca tcccacgctc aacctggacg atcccgaccc ggcctgcgca
     9241 ctggccggcc tgtcgaagga tcagcaaaca cgcaaggcga aagtggcgct gatcaacgca
     9301 tttggattcg gcagcaacaa cgcggccgtg gtgttaaaag ctgtctcctc ctgacgcctg
     9361 tccggcagga cagttttgac ggccgacgaa ccagacacga cacgaaggaa ccgggcatgg
     9421 ccactaccac agacgtttcc agcaagatca ttcaggcgtt ggcagagtat ctgaagcggg
     9481 atgcatcctc gattacgccg acgcatcact tgcgggaaga tcttggcctg gactcgatgg
     9541 cggtcattga aatgctgtat cgcatcgaag aagtgttcaa cctgcagatc cccgaccagg
     9601 atctggtcgg cctcaccacc gtcggccatg tcatcgccta cgtggaagga cgcgtggcac
     9661 cggcctcgac aaagaccgct gccaagggcg ctgcgaagcc ctctgcaaag actccggcca
     9721 agcccgctgc caagaagcct gccgcgaaac cttcgcccga gagtaagaaa gcctaacaga
     9781 atgagcacgg cacccaggcg caccccgctg acacttcggg aactgcacga ctatgtagga
     9841 ggcgaactga tcggttctcc ggacgcgacc gtcaccgccg tggccagcct ggagcaggca
     9901 ggaccaggcg atctcgcctt tgtcacatcc gagcgccatc tcaaggctcc acagaccgcc
     9961 actttcggcg cgctattggt cggacggcgc atcgcggatt gcccctcccc gcaaattgtg
    10021 gtggagaacc ccgcctacgc ctttgcgcgt gcggctcaac agtttttcgc gcgacccgcc
    10081 cgagtgcgtg gcatcgcgga gggcctcaca cgcggtgacg gcgtcacgat cgggaccgat
    10141 gtttccattt ggcctggcgt caccttaggc gaccgggtga ccatcggcgc gcgagtgacc
    10201 ctgtatcccg gagtctttat cggggacgac accagcatcg gcgacgacag cctcctctac
    10261 ccgaatgtgg tcgtccggga aggatgccgg atcggcgccc gcgtcatcat tcacagcggc
    10321 acggtgatcg gcagcgacgg cttcgggtat gtccaatacc agggccggca ccagaaaatc
    10381 cctcagctgg gcggcgtgct gatcgaagac gacgtggaat tgggtgccaa tgtctcggtg
    10441 gatcgcgcga cgtttggtga cactgtcatc aaacgcggga ccaagatcga caacctcgtg
    10501 cagatcgctc acaacgtggt ggtcggcgaa cacaacattt tagtggcaca ggtcggtatt
    10561 gcgggcagca ccaccctcgg acggtacgtg atggtgggag gtcaggcggg cctcgccgat
    10621 catttgcaga tcggcgatca ggtcatgatc gcggcaaaat ccggcgtgac cagaagtttg
    10681 gagccgaatc agatcgtctc cggcgcaccg gtgatggccc acgccacatt tctaaaagcc
    10741 caggcggtca ttcctcagct gccggaactg cgcaaccggg tccgcgaact cgaggaacgt
    10801 ctcgcaactc tggagcaagc cgccaagccc gccgccaaaa cccgcaagcc tcgccgtaag
    10861 tagctcgcac accgatatcg tcagggtttg agcagcgtct tccagaaaaa cagcttggcc
    10921 cagaagaatc cggcccacgg caggaagcac gccggaatgg gatggactgc gccatagagc
    10981 atgctgagca tcgatccggc aagcaggacc gccaggccga ccatgtaggc acctggagcc
    11041 catggcgagc cactggatga ctcctgattc ttcccgctct cccgtttagg tttcccgctt
    11101 ttcgtgagcg cgggctgccg catgcgctcg gcaaagacct caggacgatt ggccagaatc
    11161 cagcgatggt acgtggtctg cacccacccc aagagcccgc ccagcagcaa caagcctgag
    11221 ctttcgacaa aggtggtcca cgaataggtg tccgttgcga tgagttggta cgccagcccg
    11281 ccgacgccgc cgagcatgga aatcaggccg agaatgccgg ccggaaagag catgtacgct
    11341 ttgatatagg ccgtcgcgcg cgcgtcgtgc ccttcctgct gtttgacgga gacaagtttt
    11401 ggcatggccg tgatggtcgt ataatcccca cctcaaccgc aagagaaatt gtacagattg
    11461 acgagatatg ttttgtgtgc gccccacgtg agcggcatca cacggcctgt ccacaagaac
    11521 tgtggacgga ctgtgcgcaa ccatgtggat ggaacgcttt tcgtcggact acgagggggc
    11581 gcagatcaac tgcctaagga ttaggcgggc agaatcggtg agcagagaga accagggtcc
    11641 gtcagtggac cacgacgcta ggccatcggc agaatatcaa aaatggccgc ccgttcgacc
    11701 cggccgacat ccacccattg ctgggccaac ggaataagcg tcgccaagcg ctcttccact
    11761 gcagccagcc gctcgcgaat aacggccggc ttatgttgcg cggtcaccct gagaaatgtt
    11821 cgcagacctt gcagaaagac actgatattt tccgcggaaa ccttttcgga cgcctcgaac
    11881 agcacatcaa tttcttcaaa aatcggctcg agagcctgag ggggcaacac gcgttcttcg
    11941 ctcgcccgag caatacggcc gagcgtcgag accactgaag gaacccgagc ctgcatgtga
    12001 ttgagaaatc gttcatccaa ctcatccaat tcctgcagca ggcgaccgat ggcccgtgca
    12061 tccaccacgg gcgcgccctg agccagctcg cgttcggctc gttgaatgac cgtctccaac
    12121 acatcgcgca ctggctccag cgagcgtccg cgtgcgacat gcaattgtcg caaggcatcg
    12181 agaatggatg acgccggcat ggggcgcgga ggagcgtacc ggggcgtagc gatttcagag
    12241 gcgggttcgc tggaggcctg cgactccggc aactccgtcc gcgccggttg ttcaaggatc
    12301 ggctgttctc gctcaacagt ctcctcggct ggcgcaggat gttcttcaat cggtcgaagc
    12361 tcctgtaccg tcttgctgat ctccagcaac ccttctcgaa gggatgccac gtgatgcgcg
    12421 gcaacggccc gttcatgcct ggcagccgcc tgcaggagtg gaaagagcct ggtcgccatc
    12481 gtttccacgg ccgtcaatcc gaccgtggcc gcggaacggg cgaggttgtt cacgctttgc
    12541 cataaaatgg aggccaattg tgcgcggcgc gcctgctcac tggtggcatc cagacgttcc
    12601 agcgccgttt gaatctgatt gagccattcc cgcgcttcaa ggacgaacag gcctaagatt
    12661 tcccggcgta cagggatcaa cggttctgcc gtctccgcct cagccggtac ctgggccggt
    12721 cccctggcta cggctggctg gtccacggca gcctggatga ccccggcaat ggcgtcgaga
    12781 ctttccagca gggccgcaaa ttggtctgag acgtttgttg gcgcagcaga tgctgctggt
    12841 gcagccccgg ccgcttcgaa tgtgacacga tccaacgccg gcggctcgac ggtatccacc
    12901 actgacacat ggggcaagag actcaaatca agcaggtgcg ggggttctga aggcgccgca
    12961 gtctctgtgc tcgtgatgcg caagaccggc cgcgaggggt accgggccgt gattcgtacc
    13021 actacccctt cttgtccgct gctgagccgc accctcgtgc cgagtggata caccgacaac
    13081 tgttcgacca acgctttcat gatttcccgg ggaaacgccg tgcgttccgt cgtcagcaat
    13141 tcgcgcacgg cttcgtgcgg cagcaatcgt cgccgatagg gacgtggact caccagggca
    13201 tcaaagatgt ccaccaagcc gatgatttgt gccagctcgt tgatctctcg acctttcaag
    13261 cgatggggat acccctgccc tttcccccgc tcatgcgctt gcagcacgac ttctgccaac
    13321 catccatact ccgtccccac acgtctgatg acttcggaac cgagacgtgg atgttgctcg
    13381 accaacgtcc gttcttcggc tgtcaggcgc ccggccttcg tgaggagctg ctgcggtacg
    13441 gcgaaaattc cgatgtcgtg cagcaatccg gctacggcga ggcggtgtaa ctcgacaccg
    13501 tagtacccaa gaccgatccc aacctttgtc gccagaatgc cgacattgat gaggttggtg
    13561 actagcggag gcccgctggg actggagagc gcttccacga ccagttgatc gctgccctgt
    13621 aacgattcga cgatactgtc tgcgagatgc gtgagctcgg tgagatctac gtcttcctgc
    13681 cgttggacgg aggcagccac cttcgccagg atctcctctg cgtgccggta gtggttctgt
    13741 tccatacttc tttcttcaac tccccgcctt ggctggctaa atgtggccgt cgggagtcgc
    13801 aatcgcgacg gatctcactc gaatagattc agagtcattg tgacatcgag cctgtggtga
    13861 gcatgatcgc cgattcaacc ggtccgctgc ggaaggtttg cgacggcctg ccggtcgctt
    13921 cgcaccgtta ggtcgagggg gcgagccggc tcatcatgtc gaactctttt tgcccgtccg
    13981 caatccactc ttcgaccatg ctggcgacgg ccagaatgcg ggtctcgacc gattggagcc
    14041 gatgagccgc aatatcgatg cggcgttgga cgaccaatgt gaggaaattc tgcaggccgg
    14101 tcaggaacgt gacgagcggc gtggcattcg tctgcttggc aatctcgtga agacttgcaa
    14161 tcatctgtat tggagcatcg aaaccggcat cggaggtcag gacggcaacc ccgccggctc
    14221 ggagccggct gaggtgcagc gcaatgccag gcaagtcctg gcggagcgac ccgagatatt
    14281 gggcgtcggt attgtgaatc gtcagtaaca gctcctgaaa ctgtgcagcg gtaatctggc
    14341 cagcgcctat acgcgcttcg tgttccaaac gctgcagcac ctgcgggatg aggctgcggg
    14401 acatgctgcc ttgcgcggcc tgttcatcct gcagctggtg cagtgcattc aacaggggaa
    14461 gaaactccaa caccggttca ggcgccggcg ccgcctccgg caagggatca atgtcgagcg
    14521 tgattccggt ggccactttc acggacgcga tgacggtccg aaaatgttct cggaccgtgc
    14581 tgaagtcctg tttggttgcg gtggtccggt ctttgatcgt ctcaatgaac ggaaggaggg
    14641 caaacgtggc gcgttcgaca tcgggcagac tcaccgtggc agctgagccg ccgaggctgg
    14701 taatgccccg cactaccgcg tcgattaatt gggaatgccg atccgaatcc ggcaggctct
    14761 cgagttcgac cagggccgcg tggatctgca caagccactc ctgcgcctcc tgcccgaaca
    14821 gctcaatgag ctctttctgg aaatcgtctt gggtttgctc tggttccgtc gacaccgtgg
    14881 atcctgcctc tgacgcatgc acccgcctgc taccggacga gtgacggcct cttagcgttt
    14941 ggcatttccg agttgggaag cgaggcctgc gatttcgccc agttttcgcg ccagatcttc
    15001 aaatcgtcgc gtagactgtt cagccatcga ttcggcatca gcgacttgtt ttgtgagttg
    15061 catggagtgg tcccgctcgg cactcagctc ctgctccaag tccggcacca gctgagcctt
    15121 ctcttccatc tgcttgtatt gcgccagaag cgcagctgca gcctcacgtt cagcggtgag
    15181 tgttctttca agttctgcaa cctgagcgcg agcgtgttca cccgcctctt ccaataccgg
    15241 caccatggcc gcactctttt ccaaatgcgc ccgttcgttc cgcagagcca tcgcctcttc
    15301 ccgctcgctc gccagcaccc gctcgagctc ccgcatgcgg tcttccacgt gctcaaggct
    15361 ggtaatctga ttgaccagtt gattggccgc ttcacgctcg gcttcgagat gcgcttccaa
    15421 ctcaacgatg cgcgcaccaa gacgggcgct ctccgccgct tgctgcgcca gctggtcggc
    15481 gcgatgccgt tcttcgagca acgcggcttc cacctcaccc aaccgatgcg cctgcctgga
    15541 cagatcttcc agctgttgcg tcatcgactc ccgttgggct cgctcttttg caaggtcctg
    15601 ctcgagggct tgcatccggg aggacacatc cgccatctgt tcgcgttgct ccgacactgt
    15661 cagctgatgc tgctctcgct cctcctgact gtgtcgacgc tccgctccca gctgtgcttc
    15721 cagttcggca attttggcat cccgatcggc cagcagcgcc tggtgccgat catcagcctg
    15781 catcacaggg ctcggctcag cagcctgatg gattgaaatg ctccccgcgc gactttcctc
    15841 gatccctaca ggtccggccg gcgtttggtc cagaacgatg gccaccggtt cagccggctg
    15901 ttgccactca cgctggacta acgggattga cggaagctct tcctgcgcag gtgcgacggg
    15961 cgctggcgca acctcagcca tgaccggagg ctccgcaatc ggaatgcgaa tcggttcagc
    16021 caccggggcc ggcttgtgcg gtcgcttggt cagcaaatcc aacacgcgat ctttcagaga
    16081 agatccctga aacggcttct tcaacacacc gtcggccttg caggactccg cctgtttgga
    16141 cacctcgtcg ttcacaatgc cgctgatcag tagtacgggg agatcggcca ggttggcttg
    16201 cccgcggatg aacgcgcaca cttcgtagcc actcttgtcg ggcatgatga catcggagac
    16261 gatcagatcc ggacgatctt ttgccagcat cgccagggct tctgatccgt tggccgccag
    16321 cgtgacgccc attccggcct cagtcaaaag tcgttcggct accttgcgca ccgcaatgct
    16381 gtcatcggca atcaagatct ttggcatgtc attcctctac gcgatgtcgg atgcatcaag
    16441 ccggacgctg tgatgcgacc cctaaagttg ccaggttgat gagcggcagt tgttcgtcgc
    16501 ctgcgacaca ggatccggcg atgtccggat cgaggccgct ttgatactgt acccctgagc
    16561 gcggcaccac atgcagcgat ggcacctgag aatcgatgca aatggcaaga ggaccgtcga
    16621 catgtttgac gatcagacag agcgactcct cttcgcgcac cgtacggttg aattttgctg
    16681 caagatcgaa gaccggcagg cacgattcct catggcggac cagcgaggtg acaagcggcg
    16741 tatcactggg aacagcggtc gttccaggcc aagggaggac accgccgatc tcttcggtcc
    16801 tggcagccag ccgttttccc gccacggaaa acaccaccat attttgcacg cgcgcatggg
    16861 cagcacctga ggccgtcgtg tggggatgtc tgcgtaattg gctacgtagc atgatgaccc
    16921 tcgacgctta actcacaggg ctcgtcgaca tcgacatcgc ctcatccaca cccaccatcg
    16981 gcacatgcag ccgggaatca ctctcgccgg ccttgctctc cgcaaatcga atcgcaaaca
    17041 tctccagagt ttgcgccctg agcggaagct caagcaccca caatggattg gcaatgagcg
    17101 cgacaaagga ggcgccgaca aaaaatccca gcaatcgctc ccgttccttg cctcgaaact
    17161 gaggcggcaa gggatgaatc agcgctcgct cgacgtccag caaacccaca actttatcaa
    17221 ccgcaaagga tcgcgatcgt tcgtcattgc catacagaat cagcctggtg tcggaggaca
    17281 cacctttgga gaccaccttg agtcgagcgg acagatcggt tcgttcgtac tccacgccgg
    17341 cccacgtgac cagcccgtta gggccggccg aagctggcat cacgatgccg cgaacccact
    17401 gtgcaggaaa ggcgacggat gagccaccga tcagcgtgac aagaaatcga accgcttgcc
    17461 cgctcgaaat ggtgactgcc cgttgtcctc gaagccccat atgtgtcctt cattctgcca
    17521 gcaacggcac aagtcggcaa aaccttgcta tttttcggcc cgcgcctcaa caccggtcca
    17581 cttgcgcact tcctgaatca acgcccgttc ttcaaccggt ttggcaatgt agcccgatgc
    17641 cccgacactg accgccatct ggcgatgttt ttcacccgcc cgcgtcgtca tcacgagaat
    17701 gggagtggca tgagtttgag gtctcgcccg gagggcttgg atgacctcat acccgttcaa
    17761 cttgggcatc tcgaggtcgg tgataatcag ttggtaggcc tgcaccatcg ccttgcggca
    17821 accttcttcg ccgtccgtcg cggtctccac ggtatacccc gcggcttcga gcatgcggcc
    17881 cacaaacttt ctgatgctga gcgaatcgtc aatcagtagc aacggtaacg ccgagggagc
    17941 actcgctttt ggctcactat gccctgtagc atcctcaatc gctgcgaccg aagaatcctg
    18001 tgcgatcgac atagtttcct ggtactcacg cgtggtcagg cggctgacgt ccagcaccag
    18061 caccacccgt ccctcaggat cgatcgtcgc gccaccgaac acggagcgtt catacggctt
    18121 cagtccaccg agagacttaa tgacgatttc ctgccggccc agcaactcgt caactgcaca
    18181 accgagcgcg ccggtcgagg ttcgcacaat caccaccgga gacacaccgt ctgtgatccc
    18241 cgcctcgcgt cgaatgaggc tgcccagcgg atagacttcg atggcctcgt ccccgatttg
    18301 aaccacctgt cgatcaccca tgtgttgaag cgttgacgcc gtcgacatgg tgacttcacg
    18361 cacactcggc agcggaatcg catagcggtc ttttccgacc cgcacgagca aggccgtggc
    18421 gatcaacagc gtgagcggca aatgcatggt gaacttggtg ccctgtccgc gcaccgactc
    18481 cacttcgata tggccgttca tcgtctcgat gacgcgtttg accacatcca tcccgacgcc
    18541 acgtcccgct tggtcgccga cggcgtcagc ggtggagaat cctggcagga agatgaattt
    18601 gatgacttcg ctctcgggaa gggtttcagc gacatcctgc ctgacgagtc ccaacttgac
    18661 ggccttcgcc ttgatcttgg cgatgtcgag acccgcgccg tcatcttcga cctcgatcag
    18721 aaccgagttg ccgcgatgcg cggcgtgcag gtaaatcgtg ccggtctccg gcttgccttg
    18781 gacacggcgg acggcggccg gttcaatgcc gtggtacacg gcatttctga ctaaatgcac
    18841 cagcgggtcc accaaccgct ccacgacgcc cgtatcgatt tcggtatgtt caccggaagt
    18901 gaccagattc acgtcttttc ccgtagccct agccatttcg cgcgccgcac gccgaaaacg
    18961 ggtaaacgga gtgccgatgg gcaccatacg ggcacgggcg atttcatcgc gcatcccgag
    19021 cgtcaattgc tgcagcgatc ccatgtcgtc ctgggcgcga tggattgatc ctgacaactg
    19081 cgacatcgat tcgctgatgt ctgccgtcac ctcgctgatg cgacgagcca gaatattgaa
    19141 gtcatcgtac ttatcgaatt ccagactgcc gaaatcgctg acaccgggaa agccttgagg
    19201 catcgcttct ccctgggtgg tgggagatgg ctgaaacgag aaggtgtgtt tgtcttcgaa
    19261 cgtgcgtacg gcgtcggtga gtcgattctt attggccaac acttgcgtag ccaattgatc
    19321 cagggtgcgc aagcgctgtt cgagacgacc gcgatcaatc accaattcgc cgaccagatt
    19381 gaggagccgt tccaagcgat cacggctgac ccggatcacc tcacgctctt ctccggcctt
    19441 cccctcagcg gcattcaccg gctcagcttg ttcagcctcc tgcacatcaa gcgcaatcac
    19501 cgcttcggtc tgcgatgaca tgacaggctc atggactcct gccggtgcag cttcacctag
    19561 ccccttcagt tcttgtagcg aggcggcaaa ccgctgccgc gtccgagtga ccaacgacgg
    19621 gtcgcgccgc atcagggctc gaatgacgtc gatggctcgc aggaggacat ccgtatggcc
    19681 gggaagaaac ttcaggcggc cctcgcgaac cgcacccatg aaatcttcga tgtggtgtgt
    19741 gaggtcgccg attgattgga accccacggt ataggctgac ccctttaacg tatgggccgt
    19801 gcgaaacagt tgatggatga tgtcagggtt tgccgcgtcc ttgtccactc gcaacaagtc
    19861 ggtctcgaga ctttccagat attcctgcgc ctcgggagcg aaataggaga tgacttcggc
    19921 atccagcatc ggaatgagat acgcctccga caattcggcc gggtgattcg gctctgagag
    19981 ctgggctgcg ggatttagag gcttcgcggc aggagggagt gcttcctcgg cagcaacact
    20041 gctgctcgat gccgcgggcg catcaacctg tggagcttgg gtcatgcctt gctcgagctc
    20101 gcgaagcatc gtcgtcatac aaggcacgtc ctgccgcaac cgtggtagct gctgcgcgtc
    20161 tcgcgccatc aacgagcgca tcacgtcgac cgcatgccga atcgtcgcaa tccactgagg
    20221 tccaatgacc gccgctccct cacgaaccgc gatcatgcag gtttcgatcg ggaatgccac
    20281 atcacccacc acctggaatc cgaccgtgta ggcggagcct ttgagtgtat gggcgacacg
    20341 atacagctgg tggatcgtct ccgcgtcaga caggtcgagc tctaaccgct ggagaagcgt
    20401 ctgaatcgta ttgagatact cttccgcttc aggggcgaag tacgatagaa cttcttcgtc
    20461 catcacaggc aagagataat cgtcagccgg ctcggtcgca atgggcaggc actgggagga
    20521 gtcgtctgca gacgcggccg gcaggaattg cgcgcaccgt tgcacgaacc cttcaacgag
    20581 agacgcatct tcgccttcgc cccggccgat ccgtgtcacc tgatggcgga aggaggcgac
    20641 gatatcgcgg atcacttcaa aggccgccgg ccactgactc ggagacattt cctgcacatg
    20701 ttccagcgtc tcttcgagta acgcgccgag ttgccccaga ccgggaaacc catacaaaag
    20761 tgccgcacct ttgagcttat gaccgacggc atggaactcg gccacctcgg ccggttcggg
    20821 acgccccttc ccttccgggt gcaaggcttt ccagaaccgt tccatatcgt cgccggcttc
    20881 ggccacgaag atgtccaaca gctggtcgcg atcaaaatca gcgctcatag ccctacttca
    20941 tatccgagcg gtgagtaggc gccgctcttc tagaattctg ctacgccagc ttgaactgag
    21001 cgactgagga gttcaggcct tcggccaact tggccatatc ctcgatcgtg agtcgggtgg
    21061 agtcggtttg tttctgcgtc gccacggcgc cgccggtgaa ctccttaatc gcgcgaccga
    21121 ctttttccgt cgatgtggtc tgctctgacg ccgcactggc gatattctga gccaactcgg
    21181 acgagcgttt cgcaatgtct gaaatttcgg cgaacacgtc accggtccgg agagccgagg
    21241 cagagccggc ttccacggcc tgtgtttcat gttccatcgc gaccaccgca tcctgcgttt
    21301 ccgtctgaat cactttaacg aggtcggcaa tttcacgcgt tgcttgcgtg gaactttccg
    21361 ccagctttct gacctggtcg gcaacgacgg cgaatcgcgc accggcctca ccggcgcccg
    21421 ctgcctcgat agcagcgttg agcgcgagca ggtttgtttg gttggcgata tcacgaatgg
    21481 tcgacacgat ctgggaaatt tcgagcgaac ggtcaccgag gcccttcacc tgcttcgaca
    21541 tgcgttgcac cgctgaacga atactttgca tgtcgcgcac cgtttcctgc accgcgacgt
    21601 ttccgcgttc cgtggctgac aacacctgct tcgcagaatc tgaggaggcg cctgctgtgc
    21661 tggcgacctg acgcataccg gccgcgagct gttcgaccgc gcccagcgtc cgtactgatt
    21721 cttcggcctg cgcgcgggcg gttccggaca tctgtccggc agaatcccgg agcgtaccgg
    21781 ccgacttgtt cacacggtca gcggcttcac gcacctgttt caacagttgg ccgaatcgcg
    21841 cgatcatgag gttgaaaccg tcggccaggt ttccgaacat gtcggcggtc acttcacctc
    21901 gacgggtcaa gtctccttta ccgacctcgg agacgagtac gaggaattgc atcaggcgtt
    21961 tctgcatcaa gtcgcgttct tcctccgtcg ataccagagc cgtaatacga tcgagcatgg
    22021 agttaaacgc tgtggccatc tgccctaact catcatggga ttcaattttc gcacgcgctt
    22081 gcagattgcc gccggcggcc tgtgtcgcga cattggcgat gtgggaaatg tttcgggaca
    22141 agaaggtagc caggaggtag ccgacgaaca acccgagggc cacggcgacg agaccacctg
    22201 cgacgagaat gaacgtaccg ttggaggcca gggattgggc gtcgtcgttc agatccttcg
    22261 ccaccgattc gatgctgctg acctgctcgt tgtggcgtcg tacggcgttt tcgaatgagg
    22321 gggtcaagtt caccgtcaag gccaagatac cgagtgctcg catttgctca gcctgtgccg
    22381 gagatagcgt attgcgatcg aaactgtcgg ccatggcact gagcgcacca tccgcttcct
    22441 gaaaatattt tttgagggcc ggctcgaaca gggtgaggtc cttcatttcg tcgcggcctg
    22501 atcgagacac tcgcaaattg gtgctcttgt agttgttgat ggctttttcg atttcagcct
    22561 tcaagggagg aagcttggtg atttcctgcg cgaagtcact ttgtttcgtg gcagaggcga
    22621 cgtccagcag aatctggtga tgcttggtga gcgctgttcc catctgcgcg aattccgcca
    22681 gcggcaccgt gtaatccacg tacacggttt gcgactgggc actgagctgg cgcagggtga
    22741 acacaccgac gctggccatg atcatgatga tgaaactcac cagaccgaaa ctgagcagca
    22801 gcttcgatcg tgtcttcata tcctggaatc gattggtaac gccttgcccg atcatggtgc
    22861 gctcctcctt gttactgctt tctcaatgtt cgctggccgg tcgcacaatc cctgctcgcg
    22921 ctgacccgca aaccgtcata gctttattca ctacacgact gacaatgccg cactcccccc
    22981 gtccacttgc gcgaagacct gcggcacctc caggacgcct cccagccggt gatccacgcg
    23041 cagcacggcc gacacacagg gtctcgcacc agccggtccc gcctgcatgg caggcaacag
    23101 atgctcgcgg ggcaccgtat ggatttccgg aacgtgatcg acgagtactc ccacctgcct
    23161 cgcgccgtga cgtatcacca ccgcgaaggg tagcgccgtg ttgttcactg acaagccaag
    23221 gctcccgcgc agatcgacca gcgtgatgac cgttccccgg aggttcgtca ctcccgtcag
    23281 gtaactgggc atgctgggca cggccgtcac cgcttcgact tcgaacactt cgctgacatg
    23341 acgaagatcg atcgcgaaga gttctcctcc cagcgtcaac acgcacatgc gcagcgcgcc
    23401 ttcggtcgat gcggcagatg tcctcgatga accggaggat ccctgagccg gagaagtggc
    23461 agaagcctga ggctggatga ttggaacgtc gctcatgagt tagttcaatg cccgtttgac
    23521 cgcggcaact aaatcttcgg ccttgaacgg cttcaccaca tacccgttcg caccctgctg
    23581 ctgtccccaa aacttgtcgc tttcctgtcc cttggaggtg cacaacacaa tcggaatacc
    23641 tttgaaccgg tcatcgctct tcaaatcgcg gcaggcctgg aatccgttac gcccgggc
//
LOCUS       DAOQEW010000038        23680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_518_length_23680_cov_9.368254, whole genome shotgun sequence.
ACCESSION   DAOQEW010000038 DAOQEW010000000
VERSION     DAOQEW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_518_length_23680_cov_9.368254"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..743
                     /locus_tag="PLS35_06225"
     CDS             264..743
                     /locus_tag="PLS35_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNI18288.1"
                     /translation="MLKLSKKADYGLMALQHIASNQYGDVAHARVVNTKEIAEEYHIP
                     VELLAKVLQTLSKSGIIESHNGPKGGYLLGKNPREITIAHVLESLEGPLGIMDCSHEK
                     DGDACMQREHCNIRTPLLKIQNSIYQLLNNMTLQDMLGGTPLITIQSVATEQQGVER"
     gene            740..1957
                     /locus_tag="PLS35_06230"
     CDS             740..1957
                     /locus_tag="PLS35_06230"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNI18289.1"
                     /translation="MKFPIYLDNHATTPMDPRVLETMLPYFTEKFGNAASRNHAFGWE
                     AEEGVETARKQIAKLIHADAKEIVFTSGATESNNLALKGVVEMYHEKGDHIITSSTEH
                     RAVLDTAKTLETKRGVKVTYLPVDKFGMVNPEDVRNAITDKTILISVMFANNEIGTIN
                     PIKEIGKIAKEKGILFHCDATQGVGKVPINVQEMGIDLMSFSAHKIYGPKGIGALYVR
                     KKNPRVRIAAQMDGGGHERGMRSGTLPVPLIVGFGKACELCEQEMAADAARLSVMRDR
                     LHATITKALEDVYLNGHPTERLPHNLNISFAYVEGESLLMGCKEIALSSGSACTSATL
                     EPSYVLRALGVGAELAHSSIRFGLGRFTLDEEVDYAAKKIIETVTKLREMSPLYEMAK
                     EGIDLKTVQWAAH"
     gene            1998..2381
                     /gene="iscU"
                     /locus_tag="PLS35_06235"
     CDS             1998..2381
                     /gene="iscU"
                     /locus_tag="PLS35_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNI18290.1"
                     /translation="MAYSEKVVDHFNNPRNMGSFKKEEDGVGTGIVGAPECGDVMKLQ
                     IKVQNDTIVDAKFKTFGCGSAIASSSLATEWLKGKTVEEAGKIKNTDIVQELNLPPVK
                     IHCSVLAEDAIKAALADYQKKADSK"
     gene            2402..2761
                     /locus_tag="PLS35_06240"
     CDS             2402..2761
                     /locus_tag="PLS35_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNI18291.1"
                     /translation="MDVTTNTDAQAPVITLSDAALKEVKRLINVQGIEEGGLRLGVKG
                     GGCSGLSYTINFDEKIGQYDQVYDFDGVKVIVDAKSAIYLQGTQLDFQKDLMGGNFKF
                     VNPNANKTCGCGESFSA"
     gene            2776..3492
                     /gene="hscB"
                     /locus_tag="PLS35_06245"
     CDS             2776..3492
                     /gene="hscB"
                     /locus_tag="PLS35_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNI18292.1"
                     /translation="MSGHTHNADDRRELQMARSMCWHCQSEMAGEYFCDRCVKVQPVS
                     KDLDYFTCFGLPRRLAIDTQVLESKFYELSRAFHPDFYQNKSDAEQSISLGNSAMLNT
                     AYRTLRDPIQRAEYLLDLEAGSVKDIRTSPPADLFEEILELQETLDEFRESDRSSEHA
                     STLRAKLHTDRTNLEERQRDMEAKLQQLFSRWDALQDRGEATEQARAERTLILKDMRD
                     ILSNRTYVKNIVNDLVATIA"
     gene            3498..5318
                     /gene="dnaK"
                     /locus_tag="PLS35_06250"
     CDS             3498..5318
                     /gene="dnaK"
                     /locus_tag="PLS35_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNI18293.1"
                     /translation="MARIVGIDLGTTNSLVAYMDKGTPRVISARHERAMVPSVVALTD
                     NGLIVGDPAKEHLTRNPERTVYSVKRFMGKSLADVQGELAYFPYTLTEKGGVIRIQLG
                     EKSYSPPQISAMILKELKLRAEAFLGESITKAVITVPAYFNDSQRQATKDAGMIAGLE
                     VLRIINEPTAASLAYGLQKKTQGTIAVYDLGGGTFDISILKLKNGIFEVLATNGDTHL
                     GGDDFDQLIADLLLKDIRAQHGLDLSTHPDHMQAVRLQAERAKIRLSEELKTTVSLDL
                     PDGQGRFSRELTRDQLEALCIPLVERSLGPCRLALKDSGLNPTDIDEVVLVGGSTRMP
                     LVRQRVQALFGKEPHCELNPDEVVALGAAVQADILSGGTTDMLLLDVTPLSLGIETMG
                     GVMSSLIRRNTTIPASAKEMFTTYVDGQTGVDIHILQGERELAKDNRSLARFRLKVPP
                     LPAGVPRIEVTFLIDANGILNVMAKDMRTGETQSIEVKPSYGLSDQEVERMIEDSFKF
                     AADDVSARKVIEARLDAGALITTTEKSLTDGGHLVEAEEIAKIRAALSALAAAKEGTD
                     PRAIRGCMADLEQAAKGLTVAMLDDSLKQGLQGKKISDVT"
     gene            5341..5553
                     /gene="iscX"
                     /locus_tag="PLS35_06255"
     CDS             5341..5553
                     /gene="iscX"
                     /locus_tag="PLS35_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNI18294.1"
                     /translation="MDLKWQDAEDIAIRLVEEHPDADPLTIRFTDMHAWIVALPDFKD
                     DPKKSNEKILESIQMAWHEEYQDSRS"
     gene            complement(5669..8065)
                     /locus_tag="PLS35_06260"
     CDS             complement(5669..8065)
                     /locus_tag="PLS35_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNI18295.1"
                     /translation="MSELDHLLDKPEKPRRQRRWWKIILIGLLLAIVLGGGGAAGTLW
                     YFSQDLPSLDPLQNYQPSLVTRVYSDDRQVIGQFFIERRFLKPIQEMPKSLTQAVIAT
                     EDTRFFEHPGLDIVGILRAAWTNLRHGGKKVEGASTITQQLARSLFLSAERTFDRKVR
                     ELILAYKMELVLTKEQILEMYLNQIYFGQGAYGVAAAGQTYFGKELSKLTLAESAFLA
                     GLPKSPSHYSPFKAYDRAKKRQEHVLSRMAEAGFISVADRDQAIAEKLNFRRPGSEHL
                     GPYFVEYIRQLLVAKFGEAMVYKGGLEVFTTLNADMQKAAEAAVFNGLREVDKRQGWR
                     GPLRTVDVTTLEVPAPDPSVKLAEGDMMEGVVTKIAKDHVLVQIGGTTGRLSFDDMAW
                     ASKYLKGKDPTKDAVPVKNIKQLLSPGDVIEVGVKKLDKDVVHLRLEQTPVVEGALVA
                     VDPKTGAIRAMVGGYDFARSEYNRAVLARRQPGSSFKPIIYATAMNQGMSPATVVLDA
                     PVVYEQEEEDKTWKPENYGKRFHGIVSLREALIHSHNLATVRLLDKVGIRNVIDFSRT
                     VGVVSPLAADLSLALGSSSVGLMELVSVYGVFANQGVRVEPYAVASVQDSGGRTLEQA
                     AIQPRQVVSRETAYLITNMMEDVIQRGTGIAAKAVIDRPVAGKTGTTNDFTDAWFIGS
                     TPNLATGAWVGFDDRRPLGETESGAHAALPIWIAFMKEALKQLPVVPFEIPEGVMFVK
                     VDPSTALLTDQDEQRGTVELFTKGTEPTKSAGSKIDPTEFYKLDQAQESASPSPVEPQ
                     "
     gene            8289..10169
                     /gene="typA"
                     /locus_tag="PLS35_06265"
     CDS             8289..10169
                     /gene="typA"
                     /locus_tag="PLS35_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNI18296.1"
                     /translation="MNSSAPSSTSGLHAPKGRRTDIRNIAIIAHVDHGKTTLVDAVLR
                     QTHVHRKIDDMGERIMDSMDQERERGITIRAKNASVIYKGVKINIVDTPGHADFGGEV
                     ERTLRMVDGVLILVDAKEGPMPQTTFVLRKALALGHKAIVVVNKIDRPDAVIDDVVNR
                     TFDLFVHLGANDEQLDFPIVYASAIKGIATLDLKQPGTDISPLLETILDKIPAPAINR
                     DDPFQLLVLALAQDSYKGKMGIGKIQSGSIARRQNVVTLTKDGGQVPGKISDLAVFSG
                     LERSDVDSAEAGEIVAISGLEEVNIGDTIADPGSPVALPRVTIDEPTVQMTFSVNNSP
                     FAGRDGKYLTSRHLRERLFKELETNVSLRVQETDSADRFLVAGRGELHLGVLIEQMRR
                     EGYELQISQPEVILHRDGETVTEPFEELTIQVPSEYQGPVIEEIGKRRGELRHMKLVN
                     SEGTASEMHIEYHIPTRGIIGLKNILLAKTRGTIIMHHVFSGYAPADEKALLVAPHGS
                     LVAFEAGTSTAYALFMTQERGELFIGASVEVYQGMVVGQNSRDEDLDVNVCKQKQLSN
                     MRAAGTDEALVLTPPREMSLEFAMEYIGQDELVEVTPKYLRLRKRMLNPDDRRKAKKN
                     AK"
     gene            10205..>10792
                     /locus_tag="PLS35_06270"
     CDS             10205..>10792
                     /locus_tag="PLS35_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18297.1"
                     /translation="MKIRKKAPKPSPAKPPLYVIGYRGMPPSLGELKVWYDLHYGGPL
                     TWLEPVAEGRASASHGPWRAHVITSLPDTETAQWQQVLSWDHQELGAVSLSSATPSTI
                     ADTVLVTARLARGLTLLTQGTAFDVASHEYLNPSDWNDRPLDVFVTSDHVTVQHGETD
                     DHSSEWFYTLGLTKFGLDELEVIQPRGLPERETIAL"
     assembly_gap    10793..10892
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10987..11457
                     /locus_tag="PLS35_06275"
     CDS             10987..11457
                     /locus_tag="PLS35_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNI18298.1"
                     /translation="MSDVAAEIKVGDTAPDFTLKDQDQKDVKLSDYRGKSNVVLAFYP
                     LDWSPVCQGENKCLTDDFPKFQGAHAELFGISCDSFFSHKAWADSLDLKHRLLSDFNR
                     EVVKKYGLYFEPLNCGKRATVIVDKNGKVAYVKVQEIKVAREDKDILAALAKLS"
     gene            11535..11867
                     /locus_tag="PLS35_06280"
     CDS             11535..11867
                     /locus_tag="PLS35_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNI18299.1"
                     /translation="MTGTVQDVRDENYKEFTESAGAVVAYGLATCEPCKQYDPILEET
                     AAKFPTIKIGKAKMHVPGRCREIKKAHTFETYPTTHFFAQGKLLLTREGVVEPAELAA
                     LISDHLLK"
     gene            12027..12875
                     /locus_tag="PLS35_06285"
     CDS             12027..12875
                     /locus_tag="PLS35_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNI18300.1"
                     /translation="MRALALLSYVFTWLLWLMIPEASLGYDTPPTAFPASQTEFHAVT
                     INSSKSAPMAFIPAGEFAMGSDRGQDDEQPVHRVLVKAFYLETHEVTVARYAAFLMSQ
                     KADPPFKWNEATSGAHESKPVAGVNWYDARDYCRWIGRRLPTEAEWELAARGTEGRMY
                     PWGDAQPTRSHANAGHTKWHGYDTLTNVGQFELGKTPNGVYDLAGNLWEWVADWYDAT
                     YYQFSQRENPSGPSAGPLRALRGGAWNNDAKTIRSANRAGYAPDARRNDVGFRCAKDA
                     PPSNAR"
     gene            complement(12872..13624)
                     /locus_tag="PLS35_06290"
     CDS             complement(12872..13624)
                     /locus_tag="PLS35_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI18301.1"
                     /translation="MRANHSPESTEQSSVLTIGVTDLMTSLAVIFILLFSAYVTKVSE
                     TEPQTKVKAPVQAPERNVATSTTEDMKGMLRDHFQRFDLSLDADPTDPNIVRIVVPDA
                     LLNFEFGKGTLSSVADRFLVDAMPTYAALLCGAMRDRIDSLVIEGHTDDRGSDIYNLK
                     LSQERSLNVMVKGLEVIKDSAPWAYRCFHEKTSASGRGRQDLVMDHSRALDRDKSRRV
                     VFKIRLRSGDRPGEMSQAMRQLESPAFSSRLF"
     gene            complement(13626..14876)
                     /locus_tag="PLS35_06295"
     CDS             complement(13626..14876)
                     /locus_tag="PLS35_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18302.1"
                     /translation="MMSLWEFLSQDVTLLGELHSPMLSWLGSGGLIALFMWHAGRLST
                     AISSVQTCYTRVWPTLTGLAASRKSLQNEWLTVPSLSDVKRVANQSGAQPERIDLDDL
                     HTLDKAMRREPRLEQAWLHFRKTFVVERTAWFIEPKVFATRTAAEFFPRDLLNSRLNL
                     AFYHQFPSLITGGGLLLTFLAILIGLSKLHADGSHIVGIQGLINGLAGKFLTSIVGLL
                     CANLFVLLEKSAMHRLATTQQQFVTMVDELFPRKTMEQMLENFTPGAGPTPAQSPAAV
                     GAVPGDLGDRLAGSLSDRLSPTVAALREAVEVLSRRDPGGRTGSPDRLAEELSRVMQQ
                     TMAAPIQELNQAIQTLARSVEELKQDRGVKEVSDEFEFDSLDEVPTAESEQDIQQEVE
                     PDDSMLGLRWFANWRQGASMKGAA"
     gene            complement(15083..15523)
                     /locus_tag="PLS35_06300"
     CDS             complement(15083..15523)
                     /locus_tag="PLS35_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI18303.1"
                     /translation="MKVITGVAGNPAEGYQVVGDIVGTTIRQFKPGDSAMDVAIEMVT
                     SHVGGAPVVGEDYEFLGFINEGDVIRAIDKGMDLKQARAGDIMNSAFVGVKEDTSLGC
                     VARMFEMGFQILPVVQDGKVVRSITRHDLLRAQLGLGPSVDEGS"
     gene            15705..16034
                     /locus_tag="PLS35_06305"
     CDS             15705..16034
                     /locus_tag="PLS35_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18304.1"
                     /translation="MTIPWTTALQVARKLLPVVIEKAPELIRTFERFRGAPPAAEPSA
                     SDSALSALQLQIDAQQRTIEQQADTIVELRATVSRIQRALTLARTFLAATISLSLAII
                     AYLLLRP"
     gene            complement(16152..16904)
                     /locus_tag="PLS35_06310"
     CDS             complement(16152..16904)
                     /locus_tag="PLS35_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNI18305.1"
                     /translation="MASVLLNKRIEFCASHRYHKPEWDAEKNRATFGACNNDPGHGHN
                     YMLEVTVAGEVDQRTGMVVNLFDLKRVLLQVLEEFDHKHLNLDLPYFNHQIPTSENLA
                     RVLWDKLQAQQDIGTLQRLSLYEDEDLCADLTAEAGLDVASVTRRYSFTAVHEGHRGH
                     TWDLFVSVHGPIHPETGMVTDIVALDRLVKERVLVPFEGRDLRVVLGTPSVTGEYLAK
                     AVWDRIASAVPAGSLQLVKLVQTRDLFYEYAG"
     gene            complement(16993..17577)
                     /locus_tag="PLS35_06315"
     CDS             complement(16993..17577)
                     /locus_tag="PLS35_06315"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HNI18306.1"
                     /translation="MNQLTQADLLPPDEYERQREAYRQTIIDLKRRRRISIGDKITMV
                     FENRETLRFQIQEMIRVERILDPQKVQDEVDVYNALLPAPGELSATLLIELTDAETMK
                     HWLDLFMGLDHGQKLGLRAGGEIVYGEFEGGHSHETKISAVHFVRFRPTPAMVTALAD
                     PAVRAALSVRHAGYEADAEVPWTMRQEWLADLRG"
     gene            complement(17604..18890)
                     /locus_tag="PLS35_06320"
     CDS             complement(17604..18890)
                     /locus_tag="PLS35_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNI18307.1"
                     /translation="MKELRLLQPIDPATLEQETLRIYDVCDGCRRCFNLCPSFNTLLD
                     RIDDYESDVTKFTPADHHRVVDECYYCKLCYNHCPYTPPHQYAIDFPLLMAVWKKRLA
                     AERGIRWRDWLLTRTDLLGRLNSALAPLVNWGLGQKWVRSLMQMVLGVHQERQVLHYQ
                     QETFARWWGRRPSRNAGATGKKVALFASCLVNYQATDVGKATVQVLEKNGIHVVLPDQ
                     SCCGMPSFDIGDTTAMVKAAEHTIASLKPWVDQGYDVVIPTPSCSLMVKREYANLVSG
                     EDATRVAGRTYDVCEYLMKLKREGSLAMDFIQKPGRVAYQVPCHLRDQNIGFKSKELM
                     ECAGAQVEVIEKCSGHDGSWSAKTEFFPLSMKIAQKAVRQVEQVPADVVASDCPLAGL
                     QLDQAGAQAHVGGKSAQHPIQVVRDAYGLPRDPRTS"
     gene            complement(18997..19422)
                     /locus_tag="PLS35_06325"
     CDS             complement(18997..19422)
                     /locus_tag="PLS35_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNI18308.1"
                     /translation="MGNSLKGTKSHENLKHAFAGESQANRRYLYFARRADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKSAIEGETYEYTQMYPGM
                     AKTARDEGFSELAEWFETLAKAERSHANRFTKGLDSLKQ"
     gene            complement(19451..19903)
                     /locus_tag="PLS35_06330"
     CDS             complement(19451..19903)
                     /locus_tag="PLS35_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNI18309.1"
                     /translation="MSIDLREIETRFRRHAVRVTRQRAAIYAALVETTSHPSADDLYR
                     MVTRQQPMMSRNTVYYTLGVLRNAGLIREVNVGHDMARYDGNVSMHHHLICLGCGQIL
                     DVMDDELNQLHLSSGQARGFEVQGHRVEFHGYCVSCQQAERNVSPRRS"
     gene            complement(20111..20668)
                     /gene="rfbC"
                     /locus_tag="PLS35_06335"
     CDS             complement(20111..20668)
                     /gene="rfbC"
                     /locus_tag="PLS35_06335"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNI18310.1"
                     /translation="MQITATPLSGLFQIEPDVFGDARGKFVEIFRESRYGAAGITKPF
                     VQDNFSWSVRGTLRGLHYQLAKPQGKLVMVVKGAVYDVAVDIRQGSPTFGQSYGVELS
                     EANMRQLYIPPGFAHGFCVLSEEAGFLYKCTDVYSPPDERGIIWNDPKLAIAWPITAP
                     LLSAKDQAYKCLADMTAELPRYEAS"
     gene            complement(20785..21156)
                     /locus_tag="PLS35_06340"
     CDS             complement(20785..21156)
                     /locus_tag="PLS35_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18311.1"
                     /translation="MPDLQFVLVVSALCTSGLETLNVPEDLRRKVFDACWALVSTDPP
                     PTNERERVLDLRSGTELTLDALVATIRQLFMEAGISTLTWDHPPSEPTRPSSPAAEPL
                     IDRLQKLYPDPPPTSDPSTRN"
     gene            21289..21561
                     /locus_tag="PLS35_06345"
     CDS             21289..21561
                     /locus_tag="PLS35_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNI18312.1"
                     /translation="MITITLMGQLQTTDGERDLACELPTPVTVRQVIQRQGRGLRHML
                     QLLREKKVLVTINKRIASEDSMVQDGDAVRFVGHDGAGGSGLAPSF"
     gene            complement(21668..21955)
                     /locus_tag="PLS35_06350"
     CDS             complement(21668..21955)
                     /locus_tag="PLS35_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNI18313.1"
                     /translation="MAKSMTKSQIADHLAGKAGITKKTAVQFLDDFAALAYREAKNAF
                     VVPGIGKLVLANRKARMGRNPQTGEPIKIPAKRVVKFRVAKMAKDSILGKK"
     gene            22252..22809
                     /locus_tag="PLS35_06355"
     CDS             22252..22809
                     /locus_tag="PLS35_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18314.1"
                     /translation="MAKELPILPSNAQPAEGTAPEAVIYSRVFCKNENAPPLRLLIEF
                     LKSRGQSPILPPDMDDSMLDEWAWVQVILGYARERKPIQVFCIRDRGTYQDLFEQEQK
                     QFREDLSSHDDLEATLAQEHVTRARFILTTRMIEADVTEEGYDFNGWILEFFQEHCNG
                     IVQIDQQGFFSPKGELIVDLSLPEE"
     gene            22824..23414
                     /locus_tag="PLS35_06360"
     CDS             22824..23414
                     /locus_tag="PLS35_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNI18315.1"
                     /translation="MHPPLPLFQQASHWFRRAQASLLEALPCSQGCCDCCIGIFPITQ
                     LDVLELHRGLDLLPSDRKAAIVMRAREQVAALELAFPDLRSTPNLDEWTDSNIDAIVE
                     RFADLPCPALAEDGRCSVYAFRPITCRTMGVPIESDGMVQGACRVQTAVPIIRPSPSL
                     RTDYDLLAKDEAVALSILRQAHREAGDELLLPYGFI"
     gene            23482..23558
                     /locus_tag="PLS35_06365"
     tRNA            23482..23558
                     /locus_tag="PLS35_06365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23516..23518,aa:Arg,seq:ccg)
     gene            23605..23680
                     /locus_tag="PLS35_06370"
     tRNA            23605..23680
                     /locus_tag="PLS35_06370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23638..23640,aa:Lys,seq:ctt)
BASE COUNT         5384 a         6907 c         6421 g         4868 t
ORIGIN      
        1 gacggcaatt cgacgtccgc tttttcaata tagggattcg ttccacccat ggtgcgcagc
       61 ctcttttctt ttcagtttat tttcaatgac aagtgcaatg gacgatcagc cgaccatcgg
      121 gccgcgccct cgggaagcca cctctttcta cactttggct caccggcgcc cttgacagta
      181 taaaagatca catgatagct tttgtacgac tcatttgatc ggagatcatt atagtctccg
      241 actttaaaga tcggcaatag gccatgctga aattatctaa aaaagcagac tacggattaa
      301 tggcactgca gcacatcgcc tccaaccagt acggcgatgt cgctcatgcc cgagtggtga
      361 acaccaagga aatcgccgag gaatatcata ttccggtcga actgctcgca aaagtccttc
      421 agacgctttc gaagagcggg attattgaaa gccacaacgg ccccaagggt gggtatctac
      481 tcgggaaaaa tcctcgggag atcaccatcg ctcacgtcct cgaaagtctg gaggggccac
      541 tcggcatcat ggattgctcg catgagaaag acggtgatgc ctgcatgcaa cgtgaacact
      601 gtaacatccg gaccccgctc ctgaaaatcc agaacagcat ctatcagctt ttgaacaaca
      661 tgaccttgca agatatgttg ggaggcaccc ctctcatcac cattcaatcc gtcgcgacgg
      721 aacaacaagg agtcgagcga tgaagttccc catctacctg gacaaccatg cgaccacccc
      781 gatggacccg cgggtgctgg aaacaatgct gccctatttc acagaaaagt tcgggaacgc
      841 cgccagccgg aaccacgcgt tcggatggga ggccgaggaa ggggtagaaa ccgcccgcaa
      901 acagatcgcc aagttgatcc atgcggatgc caaagaaatc gtgtttacga gtggcgccac
      961 ggaatcaaac aacctcgccc tcaaaggcgt ggtggagatg taccacgaga agggcgacca
     1021 tatcatcacg tcgtcgaccg agcatcgtgc cgtactggat acggccaaga cgctcgagac
     1081 caagcggggc gtgaaggtga cttatctgcc tgtcgataag ttcggaatgg tgaacccgga
     1141 ggacgttcgt aacgccatca ccgacaaaac cattctcatc tccgtcatgt ttgccaataa
     1201 tgaaatcggg acgatcaacc cgatcaagga aatcgggaag atcgcgaaag aaaaaggcat
     1261 cctttttcac tgcgatgcaa cccagggcgt aggtaaggtc cccatcaatg tgcaggagat
     1321 gggcatcgat ctcatgtcgt ttagcgccca caagatttac gggcccaaag gcatcggcgc
     1381 gctctatgtc agaaaaaaga atccccgtgt ccgcatcgcg gctcaaatgg atgggggtgg
     1441 acatgaacgt ggcatgcgat ccggcacgct acctgtaccg ttgatcgtgg gattcggaaa
     1501 ggcctgcgag ctctgcgagc aggaaatggc ggccgacgcc gcgcgcctct ccgtcatgcg
     1561 tgaccggctg catgcgacca ttaccaaggc gcttgaggat gtctacttga atggccatcc
     1621 gacggaacgg ctcccgcaca atttgaatat ctcctttgcg tatgttgaag gggaatcgtt
     1681 gctgatgggg tgcaaagaaa tcgcgctctc ttccggctca gcctgcacct ccgcgacgtt
     1741 ggaaccttcc tacgtcttac gagccctcgg tgtgggagcg gaattggcgc attcttcgat
     1801 cagattcggc ctggggcgct tcacgctcga cgaggaagtt gactatgccg caaagaagat
     1861 tatcgaaact gtgaccaagc tgcgcgaaat gtcgccgctc tatgaaatgg cgaaagaagg
     1921 catcgacctg aagaccgtgc aatgggcagc gcattaattt gaaaccacca tctgagtgat
     1981 attttccgga ggacaccatg gcctatagcg aaaaagtcgt cgaccatttc aacaatcccc
     2041 gcaatatggg cagcttcaag aaggaagaag acggggtggg tacgggcatc gtcggcgccc
     2101 ccgagtgcgg ggacgtcatg aagctccaaa tcaaagttca aaacgacaca attgtggacg
     2161 cgaagttcaa aaccttcggg tgtgggtccg ctatcgctag ttccagcctg gccacagagt
     2221 ggctgaaagg caaaacggtg gaggaagcgg gcaaaatcaa gaacaccgat atcgtgcagg
     2281 aactcaacct gcccccggtg aagattcact gttcggtctt ggctgaggat gccattaagg
     2341 cggcccttgc cgactatcaa aagaaagccg actcgaagta accgacagga aggagcgcac
     2401 tatggatgtc acaacgaata ccgatgccca agcccctgtg attacgctga gtgatgcggc
     2461 cctgaaggaa gtgaagcgcc tgatcaatgt ccaaggcatt gaagaaggcg gcctccgctt
     2521 aggcgtcaaa ggcggcggct gttccggact gagctacacc atcaacttcg acgaaaaaat
     2581 cggtcagtac gaccaggtgt acgacttcga cggcgtgaag gtcatcgtag acgccaagag
     2641 cgcgatctat ctgcaaggga ctcaactgga ctttcagaaa gacctgatgg gcggaaattt
     2701 caagtttgtg aatccgaatg ccaataaaac ctgcggttgc ggcgaatcgt tttcagcctg
     2761 agcgagcgag tgcagatgag tggacatacc cataacgccg acgaccgccg cgaactgcaa
     2821 atggctcgca gcatgtgctg gcattgccag tctgaaatgg ccggcgaata cttttgcgac
     2881 cggtgcgtca aagtacaacc ggtgtcgaaa gatctcgact atttcacttg cttcgggctc
     2941 ccccggcgcc tcgccatcga tacgcaagtc ctggaatcta agttttacga attgagccgg
     3001 gcattccatc ctgatttcta tcagaataaa agcgatgcgg agcagtctat cagcctcggc
     3061 aactcggcca tgttgaacac cgcctatcgc acgctacgag atccgattca gcgggcagag
     3121 tatttgctgg atctcgaggc cggttcggta aaagatatcc gtacgtcacc gccagcggat
     3181 ctgttcgaag aaattcttga actgcaggag accctcgacg agttccgcga gagcgatcgc
     3241 tcatcggaac acgcgtcgac gctgcgcgcg aaactccata ccgatcgaac gaatctcgag
     3301 gaacggcagc gggatatgga ggccaaactg cagcagctgt tcagccgctg ggatgctctg
     3361 caagatcgcg gggaagccac tgagcaggcg cgagctgaac ggacgcttat cctcaaagac
     3421 atgcgggaca ttctttcgaa ccgcacctat gtcaaaaaca ttgtcaacga tctcgtcgca
     3481 acgatcgcgt aacttgtatg gcacgcattg tcggaatcga tctcggtacc acgaactcgc
     3541 ttgtcgccta catggacaag ggaaccccac gcgtcatttc ggcccgccac gaacgtgcca
     3601 tggttccttc cgtcgtcgcg ctgaccgata acggactaat cgtcggcgat ccggccaagg
     3661 agcatctcac cagaaatcct gaacgtacgg tctattccgt caaacgcttt atggggaaaa
     3721 gtctggctga cgtgcagggc gaactggctt actttcctta cacgctcaca gaaaagggcg
     3781 gagtcatccg aatccagttg ggcgagaaat catactcccc gccacagatt tccgcgatga
     3841 tactcaagga actcaagctc cgcgccgaag ccttcctcgg agaaagcatc acgaaagccg
     3901 tcatcaccgt gccggcttat ttcaacgaca gccagcgcca ggccaccaaa gatgcgggca
     3961 tgatcgcggg gctcgaagtc ttgcgcatca tcaatgaacc gaccgccgcc tcacttgcct
     4021 acggccttca gaagaaaaca caagggacca tcgccgtcta cgaccttggc ggaggcacgt
     4081 tcgatatctc gattctcaag ttgaaaaacg gcatcttcga ggtccttgcg acaaacggcg
     4141 acacccatct cggtggggac gatttcgatc agttaattgc cgatctgctc ctgaaggaca
     4201 ttcgcgcgca acatggactc gatctgagca cacacccaga ccatatgcag gccgtacgct
     4261 tacaggctga gcgagccaag attcgtctat cagaggagct caagactacc gtgagccttg
     4321 atcttcctga tggacagggc cggttctccc gcgagctcac acgcgaccag ctcgaagcac
     4381 tctgcattcc tctggttgag cgctcactgg gcccctgtcg tctggcgctg aaagattccg
     4441 gattgaatcc gacagacatt gacgaagtcg tgctcgtggg cggatccacg cgcatgcctc
     4501 tggtccgcca acgggttcag gctcttttcg gcaaagaacc gcattgcgaa ttgaatccag
     4561 acgaagtggt ggcactcgga gctgcagtac aagccgatat tctgagcggc ggcaccaccg
     4621 atatgctgct gctcgacgtg accccgctgt ccttaggtat cgaaaccatg ggcggtgtca
     4681 tgagcagctt gatccgacgc aatacaacga ttcccgccag cgccaaggaa atgttcacga
     4741 cctatgtcga cggccaaacc ggggtggaca tacacattct ccagggcgaa cgagaactgg
     4801 cgaaggacaa tcggagcctc gcccgctttc gtctcaaggt tccgccattg ccggcaggag
     4861 tgcctcgcat cgaggtgacg ttcctgatcg atgccaatgg tattttgaac gtcatggcca
     4921 aggacatgcg gaccggtgag acgcagtcga tcgaggtcaa gccgtcgtac ggattgtcgg
     4981 atcaagaagt cgaacgcatg atcgaagact cctttaaatt cgcggcggac gatgtcagcg
     5041 cgagaaaagt gatcgaagcc aggctggatg cgggtgctct gattaccacg accgaaaagt
     5101 cattgactga cggcggacat ctcgtcgagg ccgaagaaat cgccaagatt cgcgctgcgc
     5161 tgtctgccct ggcggctgcc aaagagggga cagatccgcg tgccattcgc gggtgtatgg
     5221 ccgacctgga gcaagcagcc aaaggcctga ccgtggccat gctggacgat tctttgaaac
     5281 aagggcttca aggcaaaaaa atctctgatg tgacctgatt caccgacgcg aggagctctg
     5341 atggatttga aatggcagga tgcggaagat atcgcgattc gcctggtcga ggagcatccg
     5401 gatgctgacc cgctgaccat ccgctttacg gacatgcacg cctggatcgt cgccctcccg
     5461 gatttcaaag acgatcccaa gaaatcaaac gagaaaatcc tcgaatccat ccagatggcc
     5521 tggcacgagg agtatcagga ctcccggtcc tgatcgattc cgtacggttg ctccatcaac
     5581 atatcctcac ggacgtcgat agaacgctcg cataactggc tcatcgcagg ccatggatcg
     5641 gcctgatcga gataatctgg aggctcttct attgcggctc gacgggagaa ggcgaagcgc
     5701 tctcctgggc ttgatccagc ttgtagaatt cggtgggatc gatcttggag ccggcacttt
     5761 tcgtcggctc cgtgcccttg gtaaacaact ccaccgtgcc acgctgttca tcttgatcgg
     5821 tgaggagcgc cgtcgaagga tcaaccttga cgaacatgac tccctccgga atttcaaacg
     5881 gcacaaccgg caactgtttg agcgcctcct tcatgaaggc gatccaaatg ggcagggctg
     5941 catgtgcgcc tgactcagtt tctcccaggg ggcgccgatc gtcgaaccct acccacgctc
     6001 cggtggctaa attgggcgtc gaaccgatga accaggcatc ggtaaagtcg ttcgtggtgc
     6061 cggtttttcc cgccacgggc ctgtcaatga ccgctttcgc cgcaatgccc gtgccgcgct
     6121 ggatcacatc ctccatcatg ttcgtaatga ggtaggctgt ttcacgagat accacctgcc
     6181 gcggctgaat ggcggcctgc tccaacgtgc gtcccccgct atcctgcacg ctggccacgg
     6241 catagggctc gacacgcacg ccttgattgg caaatacccc atagactgaa accagttcca
     6301 tcaagccgac actggaagac ccaagcgcca gcgacaggtc cgcggcgagc gggctcacga
     6361 cgcccacggt gcgcgaaaaa tcgatcacgt ttcgaatccc gaccttatcc agcagccgaa
     6421 ccgtggcgag attgtgcgaa tggatcaatg cttcccgcaa gctgacgatg ccgtgaaaac
     6481 gcttgccgta attttccggc ttccacgttt tgtcctcttc ctcttgctca tagaccaccg
     6541 gcgcatccaa caccaccgtg gccggactca tgccctggtt catcgccgtc gcatagataa
     6601 tcggtttgaa tgaggacccc ggctgacggc gcgcaagcac ggcacgattg tattcgctac
     6661 gcgcaaaatc atatccgccg accatggcgc gaatggcgcc ggttttggga tcgacagcca
     6721 cgagcgcgcc ttcgacgaca ggcgtctgct ccaaccgcag atgcacgaca tccttatcaa
     6781 gcttcttgac gcccacttcg atcacatcgc cgggcgaaag gagttgcttg atgttcttca
     6841 ctggaacggc gtcttttgtg ggatcttttc ctttgagata tttggaggcc caggccatat
     6901 cgtcgaagct gagccggccc gttgtcccgc caatctggac cagcacatga tccttcgcga
     6961 ttttcgtcac caccccttcc atcatatcgc cctcggcgag cttcacagag ggatcgggtg
     7021 ccggaacttc cagggtggtc acatcgacgg tgcggagcgg gccgcgccaa ccctggcgct
     7081 tgtccacttc acgcagtccg ttgaacacgg cagcttctgc cgctttctgc atgtcggcat
     7141 tcagagtggt aaagacttcc aatccgccct tgtagaccat ggcttcgccg aacttggcca
     7201 cgaggagctg acggatgtat tccacaaagt acgggccaag atgttcgctg ccgggacggc
     7261 ggaaattcag cttttcggca atggcctgat cccggtcggc gacactgatg aaccccgctt
     7321 ccgccatgcg gctgagcaca tgttcctgcc gcttcttcgc acgatcatag gctttgaacg
     7381 gtgagtagtg gctgggtgat ttgggaagcc ccgcgaggaa cgccgattca gctaatgtga
     7441 gtttcgaaag ttctttcccg aagtaggtct gcccggccgc cgctacaccg tacgcgccct
     7501 ggccgaaata gatctggttc agatacatct ccagaatctg ttccttcgtc agcaccagct
     7561 ccattttgta ggccaggatg agctcgcgca ctttgcgatc gaacgtccgc tcggccgaca
     7621 gaaagagcga ccgcgccagc tgttgggtga tcgtgctggc gccctccact tttttgccgc
     7681 cgtggcgcaa gttcgtccag gccgcgcgaa gaatgcccac gatgtccagg ccggggtgtt
     7741 cgaaaaaccg cgtatcttcg gtcgcaatga cggcttgcgt caggctcttc ggcatttcct
     7801 ggatgggttt cagaaagcgt cgttcaatga agaactgccc gatgacctgc cggtcgtcgg
     7861 aataaacacg cgtgaccaaa ctcggctgat aattctggag tggatcgagc gaaggcagat
     7921 cttgagaaaa ataccagaga gtccccgcgg cccctccccc gccaaggaca atggcgagca
     7981 gcaggccgat gaggataatc ttccaccacc gccgctgacg acgtggcttt tccggcttat
     8041 cgagcaaatg atcgagttcg gacatgaagt tggatggttg cgaggaaaga ggtcaacccg
     8101 cgacaacccc accatacaat gcgcaccgga caaactcaag caaggcgcga atcgtcgtcg
     8161 aaagaagttt cctttccaca ttccacaatg agcttgcggg aagtcgtttg aatcaggtat
     8221 agtccatagg cttagacgcc ttcgtaggaa ggcgtctttt ttttggcact ttcacggcat
     8281 tggagattat gaacagctca gcgccctcat ccacctcagg tttgcacgcg ccgaaaggcc
     8341 gccggaccga cattcgtaac atcgccatca tcgcgcacgt cgaccacggg aaaaccacgc
     8401 tcgtcgatgc ggtgctgcgg cagacccacg tgcatcgtaa gatcgatgac atgggcgaac
     8461 gcatcatgga ctcgatggat caggaacgcg agcgcggcat caccatccgt gccaagaatg
     8521 cgagcgtcat ttataaaggc gtgaagatca acattgtcga tacgccggga catgccgatt
     8581 tcggcggtga agtcgagcgc acactccgca tggtggatgg cgtgctgatc ctcgtcgatg
     8641 cgaaagaagg ccccatgccg cagaccacgt ttgtcttgcg aaaagcgttg gccctcggac
     8701 ataaagcgat cgtggtcgtc aacaagatcg atcgccctga cgccgtgatc gacgatgtcg
     8761 tcaaccggac gttcgactta ttcgtgcatt tgggcgccaa cgacgaacaa ctcgatttcc
     8821 ccattgtcta cgcctcagcc atcaagggca tcgcgaccct tgatctcaaa cagccgggga
     8881 cggacatctc tccgctgctg gaaaccattc tcgacaaaat ccctgcaccg gccatcaacc
     8941 gcgacgatcc gttccaactc ctggtactgg ctcttgcgca ggattcgtat aaagggaaaa
     9001 tgggaatcgg caagattcaa tccggctcga tcgcccgacg tcaaaatgtg gtgacgctca
     9061 ccaaggacgg cggccaggtt cccggcaaga tttctgacct tgcggtgttc tccggccttg
     9121 aacgcagtga cgtggattcg gctgaagccg gtgaaatcgt cgccatttca ggactggaag
     9181 aagtgaacat cggcgatacg attgccgatc cgggcagtcc cgtcgcgctt ccgcgcgtca
     9241 cgatcgatga accgaccgtg caaatgacct tttccgtgaa caatagtccc ttcgccggcc
     9301 gcgacggcaa atacttgacg tcacgccatc tgcgcgaacg attgttcaag gaattggaaa
     9361 ccaacgtttc gttgcgtgtg caggaaaccg atagcgccga ccgattcctg gtggcaggac
     9421 gtggagaact tcatcttggt gtgctcatcg aacagatgcg gcgcgaaggg tatgaacttc
     9481 aaatctccca gcccgaagtc attctgcatc gcgatgggga gaccgtcacg gaaccgtttg
     9541 aagaattgac catccaggtc ccgtcggaat atcagggtcc ggtgatcgag gaaatcggaa
     9601 aacggcgcgg tgaactccgc cacatgaaac tcgtgaattc cgaaggcacc gccagcgaaa
     9661 tgcatatcga gtatcacatc ccgacacgcg gcatcatcgg cctcaagaac attctgcttg
     9721 cgaagacgcg aggcacgatc attatgcacc acgttttttc gggctatgct ccagcggacg
     9781 aaaaggcgtt gcttgtggcg ccccatggct cgctggtggc cttcgaagct ggaaccagca
     9841 ccgcatatgc actcttcatg actcaggagc gcggcgaact gttcatcggc gcgtcggttg
     9901 aggtctatca gggaatggtc gtcgggcaaa acagccgcga cgaagatcta gacgtcaatg
     9961 tctgcaagca gaaacagttg agcaacatgc gcgccgccgg caccgacgaa gcgctggtgc
    10021 tcacgccgcc acgggaaatg tcgctcgaat tcgccatgga gtatatcggc caggacgaat
    10081 tggtcgaggt cacaccgaaa tatttgcgcc ttcgcaagcg gatgctgaac ccggacgacc
    10141 gccggaaggc caagaagaac gcgaagtagc gtctaacgat tcccgccctg ctcgtgcgga
    10201 ctgcatgaag attcggaaaa aggccccgaa accttctccc gcaaagcctc cgctctacgt
    10261 gatcggatat cgagggatgc cgccgtccct cggtgaactg aaggtctggt acgacctcca
    10321 ctacggaggg cccctgacct ggctggaacc ggtcgcagaa ggccgtgcca gtgcgagcca
    10381 cggtccctgg cgggcacatg tgataaccag tctcccggat accgaaactg cacaatggca
    10441 gcaggtcctg tcatgggatc atcaagaact gggtgccgtt tctctctcct ccgccacgcc
    10501 aagcaccatc gcagacactg tgttggtcac tgcccgactc gcccgcggcc tgacgctgct
    10561 cacccaaggg acagccttcg atgtggcctc ccatgaatac ctgaatcctt cggattggaa
    10621 cgaccgcccg ctggatgtgt ttgtcacctc cgaccatgtc accgtccagc acggcgagac
    10681 cgacgatcat tcttccgaat ggttttatac actcggtctt acaaaatttg gcttggacga
    10741 attggaagtg atccagccgc gaggtctccc ggagcgagaa accatcgcgc tgnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctaggcc ttctggcccc gttgacaagg
    10921 tttttccagg cgtggtacca cgaatgagtt tgcgcagttc cgcaggtcta acctaggagg
    10981 atttcaatga gtgatgtggc tgcagaaatt aaagtcggcg ataccgcgcc ggattttaca
    11041 ttaaaggatc aggatcagaa ggacgtcaag ctgagcgatt atcggggaaa gagcaatgtg
    11101 gtgctcgcgt tttacccgct cgactggagc ccggtctgtc agggtgagaa caagtgcctg
    11161 accgatgact tccccaagtt ccagggcgcc catgccgaac tgttcggcat cagctgcgac
    11221 agcttctttt cacacaaggc ctgggcggat tcgctcgacc tgaagcaccg cctcttgtcc
    11281 gacttcaacc gcgaagtggt caagaagtac ggattgtatt ttgagccgtt gaattgcggc
    11341 aagcgcgcca cggtcatcgt cgacaagaat ggcaaagtgg cgtacgtcaa ggttcaggaa
    11401 atcaaggtcg cgcgggaaga taaggacatt ctcgccgccc tcgcgaaact gagctaagcg
    11461 ataaacgggg accgagggga tagggccata acgaggccct cccctcgatc atctgtccta
    11521 gacgaggaga gcccatgacc ggcacagtcc aagatgttcg ggatgagaac tataaagaat
    11581 tcaccgagag tgccggtgca gtggtggcct atgggctggc cacctgcgag ccctgcaagc
    11641 agtacgatcc catcttggaa gaaaccgccg caaaatttcc caccatcaag atcggcaagg
    11701 ccaagatgca tgtccccggc cgttgccgcg agatcaagaa agcgcatacg tttgaaacgt
    11761 accccaccac acacttcttc gcgcagggca aactgttgct gactcgcgaa ggagtcgtcg
    11821 agccggccga gctggccgcg ttaatctccg accatctgct gaagtaaact accctcgcgg
    11881 ccacacccag ctccgatcgc aggtcgtcga cgcgacaata cagctccccg cgttccatcc
    11941 atcccacttc ccgacgcaga cgcgatacaa tagacgtgta acgagctgct gtctgaatgg
    12001 aatgactgtg gattagcctt caaccgatgc gcgcattggc tttgctctcg tatgttttca
    12061 cctggctgct ctggctcatg attcctgagg ccagcctcgg ctatgacacc ccaccaacag
    12121 cctttcccgc cagccaaacg gaattccacg ccgtcacaat caattcttca aagagcgctc
    12181 ccatggcctt catccccgcc ggcgagttcg ccatgggaag tgatcgcggc caggacgacg
    12241 aacagccggt acatcgcgtc ttggtcaagg ctttctatct ggagacacac gaagtgacag
    12301 tcgctcggta tgccgcgttt ctcatgtcac aaaaggcaga cccgcccttc aaatggaatg
    12361 aggcgaccag cggtgcccac gagagcaaac cggtggccgg cgtcaattgg tatgacgcgc
    12421 gcgactactg ccggtggatc gggagacgat tgcctacgga agcggaatgg gagctggctg
    12481 cacggggcac cgagggacgg atgtacccct ggggagatgc tcagcccacg aggagccacg
    12541 ccaacgccgg gcacactaaa tggcatggct acgacacact gaccaacgtg gggcaatttg
    12601 aattgggaaa aacgccgaac ggggtgtacg acctggccgg gaatctatgg gagtgggtcg
    12661 ccgattggta cgacgccacc tactaccagt ttagccagcg agaaaaccct tccggcccct
    12721 cagcaggccc cctcagggca ttacgcggcg gggcttggaa caatgatgcc aagaccattc
    12781 gatccgccaa tcgcgccggg tatgcgcctg atgcgcgaag aaatgatgtc gggtttcgct
    12841 gcgccaagga tgcgccgcca tcgaacgcgc gctagaagag gcgggaactg aaggcaggag
    12901 attcaagctg ccgcatggct tgactcattt cccccggtcg atccccggag cgtaaacgga
    12961 tcttaaagac cacccggcgg cttttgtccc gatcgagcgc ccgggagtga tccatcacca
    13021 gatcctgccg gcctcgtcca ctggcggacg tcttttcatg aaagcagcga taggcccagg
    13081 gcgccgagtc tttaatcacc tcaagcccct tcaccatcac atttagtgat cgttcctggc
    13141 tgagctttaa attgtagatg tccgacccgc gatcatcggt atgcccttcg atgaccagcg
    13201 agtcgatacg gtcccgcatg gcgccacaga gaagcgccgc gtaggtgggc atcgcatcca
    13261 ccagaaaccg atccgcgacg gacgacagcg ttcccttacc aaattcgaag ttcaacaagg
    13321 catcggggac gacaattcgt acgatattgg gatcagtcgg gtcggcatcc agcgacagat
    13381 cgaaccgctg aaagtgatcg cgaagcattc ccttcatatc ctcggttgtg ctcgtcgcga
    13441 cgttccgctc gggcgcctga acaggagcct tcactttcgt ttgtggttcc gtttcggaca
    13501 ccttggtcac gtaggcgctg aacagcagga taaaaatcac ggccagcgac gtcatcaggt
    13561 ctgtgacccc gattgtcagg accgaagact gctcggtgga ttccggcgaa tgattggcac
    13621 gcatgtcagg ccgccccctt catagaggcc ccttgtcgcc aattcgcaaa ccaccgtaac
    13681 ccgagcatgc tatcatcagg ctcgacctcc tgttggatat cctgctcgct ctccgcagtc
    13741 ggcacctcgt ccaacgagtc gaattcaaac tcgtccgaaa cctctttgac gcctcgatcc
    13801 tgcttcagtt cttctaccga tcgagcgagt gtctgaatag cctgattcaa ttcctgaatc
    13861 ggcgcggcca tggtctgttg catcacccga gagagttcct ccgccagacg atccggagat
    13921 cctgtacgtc ctccggggtc acgcctgctc agcacttcta ccgcttccct cagagcggca
    13981 accgtcggac tgagtcgatc actgaggctc ccggccagac gatcaccgag gtcaccgggc
    14041 accgcgccca cggcagcagg tgactgtgcc ggcgtcggac cggctccggg cgtgaagttt
    14101 tccaacatct gttccatggt tttgcgggga aacaattcgt ccaccatcgt gacgaattgc
    14161 tgttgagtcg tcgccaatcg atgcatcgcc gacttctcaa gcaacacgaa cagattggcg
    14221 cagagcaatc ccacgatcga cgtgagaaac tttccagcca atccgttgat caacccctga
    14281 atccccacga tatgggatcc gtcggcatgg agcttactta aaccgatcag gatggccaga
    14341 aacgtgagca gtaaaccgcc gcccgtgatc aatgatggaa attgatgata aaacgccaga
    14401 ttgaggcggc tgttcagcaa gtcgcggggg aaaaattcgg ctgccgttcg tgtggcaaac
    14461 actttcggtt cgataaacca ggctgtacgt tcgacgacga aggtcttgcg aaagtgcaac
    14521 caggcctgtt caaggcgcgg ctcacggcgc atggccttgt ccagcgtatg gagatcgtcc
    14581 agatcaatgc gctcgggttg ggcgccggat tggttggcga ctctcttcac atcgctcaaa
    14641 ctgggcaccg tcagccactc gttctgaagg ctttttcgac tggccgccag acccgtcaag
    14701 gtcggccaga cacgcgtata acaggtctgc acgctggaaa tggccgtcga gagccgcccg
    14761 gcgtgccaca tgaacaatgc gatcagccca ccagacccga gccaacttaa catcgggcta
    14821 tggagctctc ccaggagagt cacgtcctgg cttaaaaatt cccagagaga catcatggcg
    14881 cagcacctcc cgctcgaaac acgcatggcc cactgcttca gaccatgtgt gttatcaagt
    14941 ttcgggccag gatgtctgtt gaagaaaaac ccaataacgc tgagaatttg atctgccgga
    15001 ctctcacaga acgatgctcg agacgaatcg gctcggcaag aattgccgac ctgatgaatc
    15061 tttttgcagt gctctgttcc ccttagcttc cttcgtctac tgacggacca aggccgagtt
    15121 gtgcccgcaa cagatcgtgg cgggtgatgg atcgaaccac tttcccgtct tgcacgacgg
    15181 gaaggatttg aaatcccatc tcgaacatgc gcgcgacaca acccagcgac gtatcctctt
    15241 tgactcccac aaatgccgaa ttcatgatgt ctccggctct cgcctgcttc agatccatgc
    15301 ccttatcgat cgcacggatc acatcgccct cattgatgaa cccgagaaat tcgtaatctt
    15361 ctcctaccac cggggcgcca ccgacatgac tggtcaccat ttcaatggcc acatccatcg
    15421 cgctatcccc tggcttgaat tgccggatcg tggtgccgac aatatccccc acgacttgat
    15481 atccctccgc cggattgccc gcgacgcctg taataacctt catgatcgcc tcccttgtta
    15541 ctcggacgaa tcgtcgtttc cggcaatcta atgcctttca ttcgagacgc gacactgggg
    15601 agatccctat acgccgccat cgtgctgcga ccgtatagct ttcaacatca tggctatgtc
    15661 atgaatagag cgagtgggct ctttcagtca gacacacaac acccatgact ataccctgga
    15721 ccacagctct ccaagttgcc agaaagcttc tgccggtggt cattgagaag gccccggagt
    15781 tgatcaggac tttcgaacgg tttcgtgggg ctcccccggc ggcggagcct tccgcttccg
    15841 attccgcact ctcagcactt cagctacaga tcgacgctca gcaacgtacc attgagcagc
    15901 aagcggacac gatcgtcgag ctccgtgcca cagtcagtag aatacagcgc gcgttgaccc
    15961 tcgctagaac cttccttgcc gcgacgattt cgctcagcct tgccataatc gcctacttac
    16021 ttctccggcc ctaattgcgt tcgatagcat aggttacgag ccacaccgac gctctgatcg
    16081 agccctggtg caggatgaat gtcgatgtaa ggagcagcga agcggaagag aggtagtggg
    16141 aggaacgtga cctagcccgc gtattcgtag aagagatcgc gggtttgaac gagtttcacg
    16201 agctgaagac tgccggcagg aacggccgac gcgatacgat cccatacagc cttggcaagg
    16261 tattcccctg tgacggacgg cgtccctaac accacgcgaa gatctcgtcc ctcgaatgga
    16321 accaacacgc gttcctttac cagccgatca agcgcgacga tatcggtcac catcccggtc
    16381 tcaggatgaa tcggcccatg caccgacacg aacaggtccc aggtatggcc tcgatggccc
    16441 tcgtgcacgg ccgtaaatga ataccgtctg gtcacagagg ccacatccag gccggcctct
    16501 gccgtgaggt ctgcacaaag atcctcatcc tcatacagag acaaccgctg aagcgtaccg
    16561 atgtcctgct gggcttgcag cttatcccag agaacacgcg cgaggttttc ggaagtggga
    16621 atctggtgat tgaagtaggg caggtccagg ttcagatgct tgtgatcgaa ttcctccagc
    16681 acctgcagca acacgcgctt cagatcaaag aggttcacaa ccatgccggt tcgctgatca
    16741 acctctcccg cgacggtcac ttccagcata tagttatgcc cgtgcccagg gtcattattg
    16801 cacgcgccga aggtcgcacg gttcttttcg gcatcccact ccggcttgtg atagcggtgg
    16861 gaagcacaaa actcgatgcg cttattcagg aggacggaag ccatagtctc acatacaccg
    16921 gatgtcactg ctgtctgtta tgtacaagag ttcctcgttg aatgccagtc taaaactcag
    16981 accacccgct cgctagccgc ggagatcagc caaccattcc tgccgcatcg tccagggcac
    17041 ctcggcatcc gcctcgtatc cggcatggcg aacagagagc gccgcacgca cagccggatc
    17101 ggccagggcc gtcaccatgg caggagtcgg acgaaaccgc acgaaatgca ccgcgctgat
    17161 tttcgtttca tggctgtgcc cgccttcgaa ctcaccgtag acgatctctc ctccggctcg
    17221 cagcccaagc ttctgaccat ggtccagccc cataaacaga tcaagccaat gtttcatggt
    17281 ctccgcatcc gtcagttcga tcaagagggt tgcgctcaat tcaccgggag ccggcagcag
    17341 ggcattgtag acgtctactt cgtcctgcac tttttgcgga tcgaggattc gctccacgcg
    17401 gatcatttcc tggatctgga agcggagcgt ctcccggttc tcgaaaacca tcgtgatctt
    17461 atcaccgata ctaatccgcc gtcggcgttt gagatcgata atggtctgcc ggtacgcctc
    17521 gcgttgccgc tcgtactcgt ccggtggaag taaatctgct tgtgtcagtt ggttcatgtg
    17581 ctatccggcc aacactggct cagttacgaa gtccgtggat ctctgggcaa cccataggcg
    17641 tcgcgcacca cttgtatggg atgctgcgcc gacttcccgc cgacatgggc ttgagcgccg
    17701 gcttgatcca gctgaaggcc tgcgagcgga caatcggatg ccaccacatc ggctggaacc
    17761 tgctcgacct gacgaacggc tttctgcgca atcttcatcg agagcggaaa aaattctgtt
    17821 ttggccgacc atgacccgtc atggccggag catttttcaa tcacttcgac ctgcgcgccg
    17881 gcgcattcca tcagctcctt tgatttgaaa ccgatatttt ggtctcgcaa gtgacagggc
    17941 acctgatagg ccacccgtcc gggcttctgg ataaaatcca tcgcgaggct gccttctcgc
    18001 ttaagcttca tgaggtactc acagacatcg taggtgcgcc ctgcaacccg tgtggcatct
    18061 tctccggaaa cgaggttcgc atactcgcgc ttcaccatga gactgcagct gggagtggga
    18121 atcaccacgt catagccttg atcgacccag ggcttcaaag atgcgatggt atgttcagcc
    18181 gccttcacca tcgcggtcgt atcaccgata tcaaaggagg gcatcccgca acaggactga
    18241 tctggcagca ccacgtgaat accgtttttt tccagcacct gcacggtcgc cttgccgacg
    18301 tcggttgcct ggtagttgac gagacaacta gcgaacaagg cgactttttt gccagtggcc
    18361 cctgcattcc gggacggccg cctgccccac caccgtgcga atgtttcttg ttgatagtgc
    18421 agcacctgcc gctcttggtg cacacccagc accatttgca tgaggcttct cacccacttc
    18481 tgacccagcc cccagttgac gagcggtgcg agggcgctgt tgagtcgtcc caaaaggtcg
    18541 gttctggtca ataaccaatc ccgccatcta attcccctct cagcggccaa ccgttttttc
    18601 catacggcca tcaagagcgg aaaatcgatc gcgtattgat gcggtggggt ataggggcaa
    18661 tggttgtagc agagtttgca ataataacat tcatcgacga cgcgatggtg atcggccggc
    18721 gtaaacttcg tgacatcact ctcgtagtca tcgatccggt ccagaagcgt gttgaaggac
    18781 ggacaaagat tgaagcagcg gcggcatccg tcgcagacat catagattcg cagcgtttct
    18841 tgttcgaggg tggccggatc gatcggctgg agtaggcgaa gttctttcac ggaagggatt
    18901 tccttatgac gctggcccag agatttcgac aggggggtga ggcggaacgc tccgccccac
    18961 ccgatcgtga tgcgcctaaa acctgcaagc aggctactac tgcttcaggc tatcgaggcc
    19021 cttggtaaac cggttggcat gtgaccgctc ggctttagct aacgtctcga accattccgc
    19081 caattccgag aacccttcgt cacgcgccgt cttggccatc cccggataca tctgggtgta
    19141 ttcgtaggtt tcgccctcga tcgcggattt caaattggct tcggtgttgc caatcggcac
    19201 gccggtggcg ggatcgccga cttctttgag gaaatccaaa tgcccaaatg cgtggccggt
    19261 ctctgcctcg gacgtgtcac ggaacagccc accgacatcg ggataccctt cgatatcggc
    19321 tcggcgcgca aaatacaggt accgacggtt ggcttgcgac tcgccggcaa acgcgtgctt
    19381 caagttttca tgactcttcg tgcccttcaa actgtttccc atactgtcct ccttcaataa
    19441 ataacgtggc ctagctgcga cgtggagaca cgttgcgctc tgcctgctga caactcacac
    19501 aatatccatg aaactcgact cgatgcccct ggacctcaaa tcccctcgcc tgcccgctcg
    19561 acagatgtaa ctgattcaat tcgtcgtcca tcacgtctag aatctggccg catcccagac
    19621 agatcagatg gtgatgcata ctgacgttcc catcataccg ggccatgtca tgacccacgt
    19681 tgacttcccg aatcaagccg gcgtttcgca acacgccgag cgtgtaatag acggtattac
    19741 gcgacatcat cggctgttga cgtgtaacca tccgatagag atcatcggca ctcggatggc
    19801 tcgtcgtctc gaccaacgcc gcataaatcg ctgcgcgctg gcgcgtgaca cgcaccgcat
    19861 gccggcgaaa acgggtctcg atttcccgca gatctatgct catgtcattg ttcactagac
    19921 aggaatgatt cctacatagc acggaggtct gccgaacagc aagacagaag caggcctttt
    19981 caaaggcgta tcaccgccag cgctggagct atttgaaagt gaagccgtga aggagcagca
    20041 gacgagatga tgcgtcagat cgaatcgatc aagggaatca tcctagcgaa gtgggagcag
    20101 caaccctcgg ctaggaggcc tcgtaccgtg gcaactccgc cgtcatgtcg gcgaggcatt
    20161 tgtaggcttg gtcttttgct gaaaggagtg gggcggtgat gggccaggcg attgccaatt
    20221 tcgggtcgtt ccagatgata ccccgttcat ccggcggcga atacacatcc gtgcacttat
    20281 acagaaaccc ggcttcctcg ctgagcacgc aaaacccatg tgcaaagccc ggcgggatgt
    20341 acagttgccg catgttcgcc tccgacaatt ccaccccgta tgattgtccg aaagtgggtg
    20401 aaccttgccg aatgtcgacc gccacgtcat acaccgcccc tttcaccacc atgaccagtt
    20461 tcccctgggg tttggccaat tggtagtgca ggcctctcaa cgtgccgcga acggaccagg
    20521 aaaagttatc ctgcacgaag ggcttggtga ttccggccgc tccataccgc gactcacgaa
    20581 aaatttccac aaactttcca cgcgcatccc cgaagacatc cggctcgatc tgaaacaggc
    20641 cgctgagcgg cgtcgctgta atctgcatga cccctcttga atggaaggac gttcgtcagg
    20701 cgcatcatac cctcttcttc tcatggggaa aagctgatcg cgtgttgcgc acgccaaggg
    20761 cgggaggcga cgaaacctat gcgactaatt gcgggtggaa ggatccgatg tcggtggagg
    20821 atcgggatag agtttctgca aacggtcaat cagcggttct gcggcaggac tgctggggcg
    20881 cgtgggctcg ctgggcggat gatcccaggt caaagtcgaa atgcccgcct ccataaagag
    20941 ctgtcgaatc gtcgcaacca gggcgtccag cgtgagctct gtgccggacc gcaagtccag
    21001 cacccgttcc cgctcgttgg tcgggggagg atcagtgctg accagcgccc aacaggcatc
    21061 aaacaccttg cgccggaggt cttccggcac attcaacgtt tcaagacctg acgtgcacaa
    21121 ggccgagact accaacacaa attgaaggtc gggcatcccc ggacgctgga tattgaggga
    21181 ttgcataatg gtaatattag tcgcgcccgc gagggggagt caatttgcat ccacacccaa
    21241 gcggcgcccc caggaacagt tgcatatcat taagaggata ggcgtcgcat gattacgatt
    21301 acgctgatgg gacaacttca aacgacggat ggggaacgcg acctggcttg cgagttgccg
    21361 actcccgtca cggttcggca agtaattcaa cggcagggcc gcggccttcg gcacatgctc
    21421 caactcctgc gggagaagaa ggtgctcgtg accatcaaca aacgcatcgc cagcgaagat
    21481 tcgatggtcc aggatggaga cgcggtgcgg ttcgtgggac atgacggggc cggaggaagc
    21541 ggcttggctc cgtcattcta agactagcgt gcagcactga gcgccggtcc gtggcggctg
    21601 ctgataatgc aagggccggt gccttacaga aagcaccggc cctggatcga cagaggaacg
    21661 agatctatta cttctttccg aggatcgaat ctttggccat cttcgcgacc cggaacttca
    21721 ccacgcgctt ggcaggaatc ttgatgggct caccggtctg cgggttccgg cccatgcgcg
    21781 ccttccgatt ggcaagcacc agcttcccga tgccgggaac cacgaaggcg ttcttcgctt
    21841 cgcgataggc gagggcggcg aaatcgtcca gaaattggac ggccgttttc ttcgtaattc
    21901 ccgcctttcc agcaagatgg tcagcaatct gcgatttggt catcgacttg gccatgcgtt
    21961 cctccctttg aacaacgaat gaaagtgaac tgacgtgtgc ggtctgcgat ctataaactc
    22021 gtacgtacag aggtggagcc ggaaatctgc gcgtggtctg cagaatcagt ccgaaaacag
    22081 ggcgagagtg tatccaaagc cctaggggct gtcaagagcc gacagatgga aacatgcggc
    22141 ccagactggc gacacgcgcg tttgacgccc ttgcgcgcca agcagatcac agagtacagt
    22201 acacgcacgc atcgcggcgc agcgtcatac ttaggaacaa gaggcgtttt catggccaaa
    22261 gaacttccca ttctcccctc gaatgcccaa cctgcggaag gcaccgcacc tgaagcagta
    22321 atctactccc gggtcttttg taaaaatgag aatgcccctc cccttcgtct gttgatcgaa
    22381 tttttaaagt cgcgcgggca atcgccgatc cttccccccg acatggatga ttccatgctc
    22441 gatgaatggg cctgggtgca ggtgattctc ggctatgcac gcgaacgcaa accaattcag
    22501 gtgttttgca tccgggaccg cgggacgtat caggatctct tcgaacagga gcagaaacag
    22561 tttcgagaag acctgtcctc ccatgacgat ctggaagcca cgctcgcgca ggaacatgtc
    22621 acgcgcgcgc gcttcattct aacaacacgc atgattgaag cggacgtgac ggaggaaggc
    22681 tacgatttca acggctggat cctggagttt ttccaggaac actgcaacgg gatcgtgcag
    22741 atcgatcaac aagggttttt ttcgccaaag ggagaactca tcgtcgatct ttcgctgcct
    22801 gaagagtagc ctgatgggga ccgatgcatc cgcccctgcc gctgtttcag caagcctcac
    22861 actggttccg acgcgctcag gcctcgttgc ttgaggccct cccctgcagt caaggctgct
    22921 gcgactgctg tatcggcatc ttccccatta cacagctcga tgtcctggaa ttgcaccggg
    22981 gcctcgacct gttaccctcc gaccgcaaag ccgccattgt tatgcgcgct cgagaacaag
    23041 tcgccgcact tgaactggcg ttcccagacc ttcgatccac accgaatctt gatgagtgga
    23101 cggatagtaa catcgatgcc atcgttgaac gctttgcaga ccttccctgt cctgccctgg
    23161 cggaagacgg acggtgcagc gtctatgcat tcagacccat cacgtgccgc acgatgggtg
    23221 tgcctatcga atctgatggt atggtccagg gcgcctgtcg ggtccagacc gccgtcccca
    23281 ttatccggcc ctcccccagc ttacgaacgg attatgacct actggccaag gatgaggccg
    23341 tagcgttatc gattctgcgg caagctcatc gggaggctgg cgacgaactg ctccttccct
    23401 acggattcat ctagctcacg catcaactcc ctagacagaa acagaaacgc tatgctaagg
    23461 tgcctggtct gaccgacggg agcgcctgta gctcagctgg atagagcatc agcctccgga
    23521 gctgagggcc acaggttcaa atcctgtcag gcgcaccaat cgctctgtca ggcacgtcac
    23581 gatcgataca tatgcactcc ggtggggccg ttagctcagt tggtagagca gctgactctt
    23641 aatcagcggg ccgtaggttc gacccctaca cggcccacca
//
LOCUS       DAOQEW010000039        23610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_521_length_23610_cov_6.270006, whole genome shotgun sequence.
ACCESSION   DAOQEW010000039 DAOQEW010000000
VERSION     DAOQEW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23610
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_23610_cov_6.270006"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /locus_tag="PLS35_06375"
     CDS             complement(<1..2549)
                     /locus_tag="PLS35_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HNI18316.1"
                     /translation="MWTIYTFGNGDVLTQILLGIRIMMTTGRFESLMELALLVFAMFG
                     LVYFVFNRRFLVPLVVGAFFILFVSVRITVDVSVEDQVNPDVPDQIVNNVPLAIALPA
                     YISGQVGYYTTQLVEASFPMPVQYTQGQTSFNRPLFDLQKVLNARLQDSDLARNTDTY
                     FMMCVFREVGLLQKNLGTLRNSPNLLNDVGAMNNALTALGYTGGTLEAAPRVCPDFYS
                     SLILPGYTSGQPDYEGAMRHLRTQLQLDPATGNEEGLTEDIRANLLLSVGQSARDMIN
                     NVLLIHAWKNAESADAKRRAATADTVLLQQEAIQKDLKFQSYSKSYVAQKVVPLLRTI
                     VEGVVYIMTPFLLVLAISPGMSRLMGTYWRLFMWLQTWSPLYAVLNFVMFWEARSRLG
                     AILSSNGVGQSVNYATLDRINEHVAMLNSTAADYVWLVPAIGFGLMWGGSQVLGSILA
                     GSRTAETVAGQVGGQFSRGLGANLEEGPSYRHEQRGAQFGEMIGHDVEPQMTVPAGPG
                     GFRVDSQSGTSEIHDSQGGITSIGANGIQNYKGPEGQYAMDSKGHLLSGVFRHKMTDP
                     QSGKQVDAQTSVFGSMVEHRFDTMGEDGARKSVLVQENQDTGELVARTEQSVRKGLSE
                     TRTIADDGSQSFSRSGNVPLFLEVAGETRPDAGFVQMMGERGPGERADTITQSMFQST
                     MGAKQSYTGRLVERDGQFHFLATEGGAIRGFDNKDTEAAVHRESFVSPDGSNYFVETG
                     QAQQMVLGKDGVTRPMEGYVRAFGSVDRSDPAHPRDIYSSYSLTSDEGGHAHTVTGTL
                     GTDASGRQFLVEATEHFESGTSGVAHDTQTVGSYTLSGTFTALSHGEANQPG"
     gene            complement(2598..3932)
                     /locus_tag="PLS35_06380"
     CDS             complement(2598..3932)
                     /locus_tag="PLS35_06380"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HNI18317.1"
                     /translation="MRCKRILVGLCMWAVLAWPPLVQAQSMNDALTNMFSSWGVGITS
                     PPGAYESQRRGYFSGGGVSVRLWQDPMRLWSIAPPRLSVGCQGIDVYLGSFSYGKLDR
                     YVQLLQQIGTGAVLGYAFQLAMKAICEDCADVLNKIEAAARALNAAGRIQPCQTGIEL
                     GKALAGNEASKQKLASRFGDAWQKMKEAGGAIGDVFEDRDAVKNQSNADAASALKGTE
                     YDVTGNLVWDVLTQAGLDQNLIVMVMSVTGTIIVGNDGDVQTRDPTMTFEQLVDSHLG
                     DVVKIFNCGGDSECLNPTITDATDIEGFESRVYTSMSNLIEDLYSGSAISAADQQIIN
                     MTPVPILTMLADYGRPRDVGNQIARLSSEVVAADMGYQWVKWAASETSRNVSYIQKVK
                     PEWPGNLQDFQGRLHQVLRGASDSLAKRQRMVNNINQLMQLTKTQGEMRSLR"
     gene            complement(3943..4803)
                     /locus_tag="PLS35_06385"
     CDS             complement(3943..4803)
                     /locus_tag="PLS35_06385"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNI18318.1"
                     /translation="MSVCSMGGPTGVARAADPPGSKTFIDHPRQGWFFYQDPPAEAEE
                     DAEESPVSVTIDGLPPGAWLEPSKYRTWFKGLAMDGVKLTALPVTVLRELVSAKKERA
                     LDDPSVENVTSYIKVQKEAYDRSQRFTDAWQIAMYTDPKLDYASQHPTSTYGHSVESE
                     IKRNTEEQLLAGTADRVGLFFFFTSTCPFCQEQSKVLKVFADTYGLTVKPVSLDGVGL
                     PEYPQPAINNGMAENVGVHMVPMIYLAIPEENFLKPLGAGLMTNADLRERLLVLLRNR
                     GLLGERPSHG"
     gene            complement(4815..5903)
                     /locus_tag="PLS35_06390"
     CDS             complement(4815..5903)
                     /locus_tag="PLS35_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HNI18319.1"
                     /translation="MTRSSMRGIRQVFLLVCLSCVLIGVATLWPSGTAEAVCTIGNPV
                     LANSLATTCWECMFPISLSGVTLFNPGEDFTAPAVGPGRAPFPPQLCGCVCLGPICIP
                     GLPLGIWEPKNLVEAVHDPLCFPTLGVSLAGGFSYIGRGMATTQMDSELNYAFFHVHY
                     FIFPLWAIVGIGLDIACLNPTGIADEIDLAYASEIDPLWDDDLISLILFPEALVLANP
                     VSLAACAADSVAAAVGFPIDPLFWCAGSFGFMYPPTGHVSGADGDLKPAALSMTRLLA
                     KLARAGTEFWTSANGPLLCDDVTTALIVKSQYKFQLLWPIPNTGIPPPCCQPLGRTLM
                     MWGLGKIVPAVGEDFVWLLWKRQNCCLL"
     gene            complement(5896..7092)
                     /locus_tag="PLS35_06395"
     CDS             complement(5896..7092)
                     /locus_tag="PLS35_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="HNI18320.1"
                     /translation="MKGSSVIACMAVTVVLSLLSIGSPALSESTGRPSKPSPEEMAQD
                     IARYSRQALRQGRPQESPKEMRRDGLYLLLSFSLPDDTLKDYLREAKLLGAKVLLRGL
                     VQESFKVTQDRIKRVLFTGERPDDSLLVGIGIDPVIYRTVGAGEVPALVFVKDEKFMV
                     ASGASSVAHLLALLSQEQARLKPWGEWFERRHRGFLQGGPTEEAPPLLPTIERSVKVW
                     ADARGADIAERDMIDVMKERVANANWPDIQRRSGDALKRHFAKGPGLALPHVEEARVA
                     FVDPTVEYPEDIKDPTTGTVLIKSGTKINPFDKVRWIRTLVFFDGTSPAQMGWVQQYL
                     REHDPKFVKLIISDGDVQKVMEQLHQRVYWANPLLVSRMGVGAVPSVVSQLGRNLRVE
                     EVAIHD"
     gene            complement(7138..7716)
                     /gene="lepB"
                     /locus_tag="PLS35_06400"
     CDS             complement(7138..7716)
                     /gene="lepB"
                     /locus_tag="PLS35_06400"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNI18321.1"
                     /translation="MKPFNALSLLNVLKRAPTILEWLDRHRWVHVGLMLFCGTIIAAG
                     VIAPWYEVAARMSGSWPHHNVFLIQKGQSVGRGSLVAFIMQQDYAERVQPGRLHRDYA
                     DVGKRWMKRIVGVPGDHILVQDNEVYINGQSVGRGIGIDRYGQRIDLATLPETIPEGQ
                     YYVALPHPRSFDSRYYGLIRQADILGTVTPLI"
     gene            complement(7646..10159)
                     /gene="traC"
                     /locus_tag="PLS35_06405"
     CDS             complement(7646..10159)
                     /gene="traC"
                     /locus_tag="PLS35_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HNI18322.1"
                     /translation="MSTILEWKAKQLVERTAISDWLPYEAWDDTNQVYRLDDGRIGVV
                     FASKPLLGLSEESIQKLTNLFESDLPIGTAFQFMLHASPIIEPYLDHHVVLAQRGQAG
                     ERYVADARKTAEFYLHARDTQLTSNPPLRPRDFTVYVSVAFPTQETTAQGWEDERIHQ
                     AVSGIEGQLNTLGLLPERVGPLQLLNVLHVLLNPGHSWESRPPAYSDQLPIRDQAVRL
                     DTAATLRTKMIELDGKFLKTLTVQSWPVQWHGGNNRELIGSLVRFTDQITCPFFLTLN
                     VLKFDQGKAKGKLRKKHVVINQQASGFLLGLIPSLRFKLDHFNGVQAQLENGRQLIGS
                     YFQVVLYGDTPRHVEEQTGTLRALYRAKDITLQEDHFIGWKIFMTGLPLGLPSDDKAL
                     IDGLRRMKTYHTEVPAHICPIVADWKGGGAPVVLLTSRSGQLITMDVFANQQTNYNMA
                     IAATSGAGKSFFMNNWIKSYLGIGGQVWVIDAGFSYVKLVELLKGQYIQYGANAQRLC
                     FNPFSKLVHWGSSAGGDDDPSDRADELATLKALHAQMASPSRPLSDVELSFIEEAIMR
                     VLEVEGREGCPDSVHRVLRGMTDPRARDLARVLASYAKGGMYSHLFNGTNNVNFENDF
                     VVLELDGLNEQKQLRSVILLQMQMNIQAVMYKKENRGRRKFVILDEAWDLLSQGGNTA
                     AFFETGARRVRKSGGSMITITQGINDYYDKMRDVGRYLLENAEFVGLLKQKTESIAAF
                     RNNGRIVLSEMEYRLLSSVTKTTEYSEIFMITPFGRGICRLTVPRETQLLFTTNPDEL
                     ALIDRVRKEGPKELSIDEAIQCIIAAERAQAGADNPRVA"
     gene            complement(10184..10846)
                     /locus_tag="PLS35_06410"
     CDS             complement(10184..10846)
                     /locus_tag="PLS35_06410"
                     /inference="COORDINATES: protein motif:HMM:NF021212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HNI18323.1"
                     /translation="MSLCVLFAGCGGYESNFSCKGYPDQATCESVSEAYEKRFAAGQK
                     VTQYTKDQKDASHDTSGNSGPGQAPSPMVFSGKPDSAVGKPVITPASALRVTVFPWKD
                     TKKRLHDQSRHYLIVDEPDFIFGHMTQGVSPGSSSWHGKDLYPRMGRMVEKRDGKRNS
                     SGNAAVNAMTENIPEAGQRAAPMPVNPLGSGGIMPQGFPQLPQSGGAANYLTDDGQLP
                     PQ"
     gene            complement(10958..11734)
                     /locus_tag="PLS35_06415"
     CDS             complement(10958..11734)
                     /locus_tag="PLS35_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNI18324.1"
                     /translation="MKQSYQQLMAGMLCAICVSVVCEPDLRAEPDDTVMPIKTVIEQK
                     FPGMKVLRVSKMEIPGWLLVESDSDRVENFMYVHDSGRYVLSGALFDIEMGRNVTQEH
                     VRDRQQALLAGMDASKTILLSPMQPKLDRPLLVFDDPDCRFCQQFHPEVQKLVEAGVP
                     VAVVLYPLVRTHPDAYRKSVAIWCASDQAEALGRALMSKPVVGEAGSCSHPIDENIKL
                     GKRLGVHSTPMVFLPNGQSFAGYRPASEVLALLQLEMEQK"
     gene            complement(11756..13042)
                     /locus_tag="PLS35_06420"
     CDS             complement(11756..13042)
                     /locus_tag="PLS35_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HNI18325.1"
                     /translation="MASIKPTESAILNPGDPIRPEKPAPSKLLAGVVLVGGFIVLSMV
                     LQQFRGNQPGIVRGKSPVKAVSINREATDKEIYMKDAGARIEALEQQRDQMRRLLEQD
                     GRDKTEMKTQLSDLQKRIESVARSGGGALAGTKSGALPPLPPPLSANPLGGASATPVS
                     TMPAPKGPIPVQSAPKPTGGALPAPGVAGGATEAFQVFRPETVPGAKKHEKKLWLNTG
                     TIIPVKLLSGMDAPARSTGAQGGGAAVNSPYPVLMMVRDIMKLPNEARIDAKDCFLVG
                     EGLGDLSSERVMIRGNAISCVKKNGEAVDIELKGFVSGEDGKQGMRGRVVLKEGALLG
                     RTLLAGFVSGISRAFMPYQSGFFVAQSPSQAFNLPPAGPVAMSGMAGGAGRAAELLAR
                     HYSMMAASIFPVIEIDAQREVDFIVGTGRELEGFFQ"
     gene            complement(13063..13836)
                     /locus_tag="PLS35_06425"
     CDS             complement(13063..13836)
                     /locus_tag="PLS35_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HNI18326.1"
                     /translation="MTSSVYRDTKHRAMAMCVLGLLLCLSRAGSAGAAVVVGDEGSTP
                     LTLSGRDLNVLTFPAPITMATSSRADLKAKPEGRNLIVQVKTGPADLVVMAGEQTYVF
                     EVVVSASLGAQTVNIEDRRAGTVPYDSDPARQAGDYVDGLLDTLQMAARGALPKGYRQ
                     TTMNEERYPKWLELKVVQGVEYRGPKYRVIVYRLVNTSGQKYTLREQEFLTGTQKAIA
                     LNREVVESGEEVVVYLLDDAPSEPPKPKPEQKVDPDAKG"
     gene            complement(13847..14428)
                     /locus_tag="PLS35_06430"
     CDS             complement(13847..14428)
                     /locus_tag="PLS35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HNI18327.1"
                     /translation="MTIELWKSKLHNAQVLNLVFAALAIVLAVALVVNGFFRQNVAVH
                     VVPINPTQEYWANAMGGDEYYKKSIAMAMLPWIANVTPASVDFHHRQLLQWVPSSHYG
                     LMSESLGAEAVYVKTNNLSRVFFVTASRVDGDMAVFEGIERRFVGKSEIREEEREYRI
                     SMRWEQGRPWVVGLSAVHLDRSLQDGQSDPAKG"
     gene            complement(14434..14715)
                     /gene="traL"
                     /locus_tag="PLS35_06435"
     CDS             complement(14434..14715)
                     /gene="traL"
                     /locus_tag="PLS35_06435"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HNI18328.1"
                     /translation="MNGTFIPRYLDSLPQIFWWEIDELAVLFVCIFLGIVTKFLTYLI
                     VVGIVSTLVLSKMKNGKSEGFLFHWAYWSGVPTFQLTGAPHGTTREMME"
     gene            complement(14794..15114)
                     /locus_tag="PLS35_06440"
     CDS             complement(14794..15114)
                     /locus_tag="PLS35_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18329.1"
                     /translation="MGDGLRKVAGDIWRMSLLLGLMVLASFVVLAVPAFAGADATFGP
                     LVTLLTNYMQGSLGTLLALVAGAIGLGSAMAGRWAGMFTGFGVAAAAFYIPNIIPTIT
                     TGLM"
     gene            complement(15227..16135)
                     /locus_tag="PLS35_06445"
     CDS             complement(15227..16135)
                     /locus_tag="PLS35_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI18330.1"
                     /translation="MAVGNTKDEPGHSGAMVKGGSMTGGRPTYMSWAGLACVGLLTVG
                     GCTTRPAVPPTMSDPTRLCGQGYTFPTTVVSVQSGPQWLLSRPMGCPEPTPLTQVPNK
                     PTVADLPPKPIKAGIHRLQGLPTFSSGTLAARPGDMSRAPGSSPDKAVIPGPTGSPTT
                     VVCRREPEVRHRRVSFAMGSADLSERAQRDLTELRIEEILYLRVEGYTDPTGSRETNQ
                     ELGMARAEAVAKVLQARVKGPVQVEVVGRGGCCFLPNHAESRRVEVMMLLQGRCADSS
                     EVERRSEAPPVTPVVSAGATHDSGKP"
     gene            16463..16792
                     /locus_tag="PLS35_06450"
     CDS             16463..16792
                     /locus_tag="PLS35_06450"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNI18331.1"
                     /translation="MKPLRFLGNSLERLRQFPEDARHDVGYQLEQVQHGKQPTDFKPM
                     PSVGKGVEELRVWDDSGTYRVLYVARFADAVYVLHAFQKKSRATSARDLEQARTRYAE
                     LKRGKHD"
     gene            16785..17105
                     /locus_tag="PLS35_06455"
     CDS             16785..17105
                     /locus_tag="PLS35_06455"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNI18332.1"
                     /translation="MTKTIHSFKSVWDALADTPEQAANLRARAALMRQITKVIETNGW
                     TQAEAGATCGVTQPRMNDLLRGRISRFSLDALVNMAAALGRPVRVELQPPKHRRVTRT
                     RRLA"
     gene            complement(17238..17636)
                     /locus_tag="PLS35_06460"
     CDS             complement(17238..17636)
                     /locus_tag="PLS35_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18333.1"
                     /translation="MRSSGQEPLVQRAEQCARSITYVAGELIRERFLLGGIVAEALER
                     SQYGDAVIVKIAYRMSTAVGKCIRPHMLYESARIYSAFGGQWERIEALRTGSDCVNRG
                     VPGAHVTTVLQQSVAQPVLESFLGVSSPVF"
     gene            complement(17834..19621)
                     /locus_tag="PLS35_06465"
     CDS             complement(17834..19621)
                     /locus_tag="PLS35_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI18334.1"
                     /translation="MNGYGSIQGSMQGKDVSAKVASCPEEMPQVVGVITSRTRARRGA
                     GDWIEIGRKHRAFVPDGIPVPSVGEVGECWGRMTANGADPVLMVSRIRSRRFEACASP
                     SELLGVLGVRRTPPLRFEEVCQLLGPLVEWLCCAGWKAVARGIVKGTLRSARAISADP
                     FLLYRRRRLPFQATVAAAQVLGHELCSDGHYKAAVMEVLARADEEGVGGISEAHLVAR
                     CAELIGLPADKVAPSVSIQMGLVGKFEQGLWFSPVVYFARKKALAMIRANQQDSGDAR
                     SSVQARSLRYRYSIVTGGAGSGKTELAKSLMTQVREQGGKVAATAMTGKAATLLGEDA
                     TTLHKLLGYGGGGYSVSTVDADLVLVDEAGMLTWHTLYRLLLACRGQVVLIGDPQQLA
                     PVGATPVMAELLTVLPVVRLGEEGSKGSLLVKVQVIRFASEALLLYQLRKIVRGYQDT
                     GVEWQALSPVYAGGLGVDRLNRWLQEIMNPDGPPCHGGFRTGDRVIVTKTRYDIGQRA
                     VNGEQGRVLGSMGDTIALRLDSGREVALRAEELRLSYCITVHKAQGSRYERVVFIIPE
                     RECGAFAVEERMQYVGRTRGREATVCMVY"
     gene            19781..20917
                     /locus_tag="PLS35_06470"
     CDS             19781..20917
                     /locus_tag="PLS35_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18335.1"
                     /translation="MLAELIHPLALMEHQEWEYRPRPSNAGPDHCIRKLTYHARHAPA
                     KSPSGRFLVVLDDSSWHEELVLNWISKSTFHLHSQQLELTCATTLFQGRPFPIKGHID
                     GVITDLLGIDRLLELKAIEHFTFTRFAEGNYPINHFVQVVFYLVGLAALNPHIDEALL
                     LIKNKNQSAFLEYRVRYDRDNDRLTVLEITHSTGARIYPKQDFVGLYQEALRRFEEVE
                     QYRDHGTLPARPYPNADNYHCDYCPYQFCWEGIEQVPVEGIAELRTELVPAVEEYLGL
                     QTELAALTPKQKRLDALKQQFKLELKTEGITRAEGHGYYIASSPYEQSKLDQSKLSKA
                     VIERATVKESYSRFSAGRLSKGIDTTKSKRRSPLKKPKPTTQIA"
     gene            20956..22290
                     /locus_tag="PLS35_06475"
     CDS             20956..22290
                     /locus_tag="PLS35_06475"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18336.1"
                     /translation="MSDAEKPTFIPMNRINGISNRRRMPIIGKIRLGVKVSGPNGFYP
                     KETPHFVVPEEVAKVFGAQPNELEVMFVSNDPTVNCPQALRWYGEAAGLQCIGNNQVA
                     RRLNQDTWKTESRSCPCEKLESGECGPRANLIFMIPKVSSGGLFQIDSSSINSMETIN
                     SYFDFLALTLGRIAMIPLKLRRVPVTRRREGRPTTHYPLELRYEGSADETARYQQETE
                     TVLKKIKMLEIQEPIEINPVLDPGAHVVMEEELEDDPGPVAAEVPGDTPSETHDPTPP
                     AMFTPSTAAAKAPEPTPLPAGPLISVAQTNYLLRLTKALPLTEDDLRMKVRALTQREA
                     SGLIKRLIAQDFSYFTSSPGTPPITPANTGAVAESMTDPQFKKIMGLVISMDIQETHV
                     KSLTSGYTKRQASTLIDQLVGGDFSAFQEMKEEELPVLVTPESAPVEEKVEF"
     gene            complement(22392..23066)
                     /locus_tag="PLS35_06480"
     CDS             complement(22392..23066)
                     /locus_tag="PLS35_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI18337.1"
                     /translation="MVLVKDLLVRMRIAQNLTQTELAEKLGCTASHISIIEGGKSSGR
                     KKILPSDDMLRRIAQCLGGSPLDVQRRTYQLLVARAREVCSPEVAELLDDQILTGMPA
                     AFLERVKQDLSEYSDEELAGVTDQVQLGGRFKQVLSGIGRLSQAEVERLAQVLGQSSE
                     TYLVEAGYLSESARSLFGRNPNCVNILQVFGSLRPETQEELLRFGEASVSLPVSERMH
                     PPTAHE"
BASE COUNT         5177 a         7638 c         5982 g         4813 t
ORIGIN      
        1 ccaggttgat tggcttcccc atggctcaat gccgtgaacg tgccgctcag ggtatacgag
       61 ccgaccgtct gggtgtcgtg agcgacgccg ctcgtgcctg actcgaagtg ttccgtggct
      121 tccaccagga actgccgccc tgatgcatcg gtgcctaatg tccccgtcac cgtgtgcgcg
      181 tgcccaccct catcactggt cagactgtaa ctggagtaga tgtctcgcgg atgggcaggg
      241 tctgagcgat cgactgaccc aaaggccctg acatatccct ccatcggccg agtcacgcca
      301 tccttcccca agaccatctg ttgtgcttgc ccagtctcca caaaataatt ggagccgtca
      361 ggggagacga acgattccct gtggacggcg gcttccgtat ccttgttgtc aaatcctcgg
      421 atcgccccgc cctcggtcgc cagaaaatga aactgtccat cccgttcgac caaccggccg
      481 gtatacgatt gcttcgcccc catcgtgctc tgaaacatcg actgcgtgat ggtgtctgcc
      541 cgctctcctg gcccacgctc ccccatcatt tggacaaatc cggcatcggg acgtgtctcg
      601 cccgcgactt ccagaaacag cgggacattc ccagatcgtg agaaactctg acttccatcg
      661 tcggcaatcg tacgcgtctc ggacaagcct ttccgaacag attgttccgt acgcgccacg
      721 agttctccgg tgtcctgatt ttcctgtacg agcacgctct ttctggcgcc gtcctcaccc
      781 atcgtatcga aacgatgctc caccatcgac ccgaacaccg aggtttgtgc atcgacctgc
      841 ttcccggact gaggatcggt catcttgtga cggaataccc cactcaggag gtgccccttc
      901 gaatccatgg catactgccc ttctgggcct ttgtagtttt gaataccatt cgcgccgata
      961 ctggtgatgc caccctggga gtcgtgaatc tcactcgttc cggattgact atcgacacga
     1021 aatccaccgg ggccggccgg cacggtcatc tgcggctcga cgtcatggcc gatcatctcc
     1081 ccgaactggg caccgcgctg ttcgtgtcga taggagggcc cttcctccaa attggcaccg
     1141 agcccacgag agaactgccc gcccacttgc ccagcgacag tttccgccgt tcgcgatccc
     1201 gccaggatcg atcccaacac ctggctgcca ccccacatca gtccaaaccc gatcgccggc
     1261 acgagccaca cataatcggc agccgtggag ttcagcatgg cgacatgttc gttgattcgg
     1321 tccaacgtcg catagttgac ggactgcccc actccgtttg aactcaagat cgcgcccagc
     1381 cggctccgcg cttcccagaa catgacgaaa ttcagtactg catacagagg cgaccaggtc
     1441 tgcagccaca tgaacagccg ccagtaggtg cccatcagcc gggacatgcc gggcgaaatg
     1501 gcgagcacca gaaggaacgg ggtcatgatg tacaccaccc cttccacgat ggtccgtaat
     1561 aacggcacga ccttctgcgc gacataggac ttcgagtagc tctggaactt cagatccttc
     1621 tggatcgcct cctgctgcaa cagcacggtg tccgcagttg ccgcgcgacg tttggcatcg
     1681 gcggattcgg cgtttttcca cgcatgaatg agcagcacat tgttgatcat atctcgagcg
     1741 gattgcccga cggacaacaa gagattcgct cgaatatcct cggtgagccc ttcctcattg
     1801 ccggtggccg gatcgagctg caactgcgtc cgcaaatgcc gcatcgcccc ttcgtagtca
     1861 ggctggccgc tggtgtatcc tgggaggata agcgaactat agaaatccgg acagacccgc
     1921 ggagccgcct ccaatgtacc acccgtatac ccgagcgccg tgagtgcatt gttcatggct
     1981 ccgacatcat tgagcagatt cggagaattt ctcagcgtgc cgaggttctt ctgtagcagt
     2041 ccaacctccc gaaacacgca catcataaaa taagtatccg tattgcgagc cagatccgag
     2101 tcttgcagtc tcgcattcag caccttctgc agatcgaata acgggcgatt gaatgatgtt
     2161 tgtccctggg tatattggac gggcatcgga aaggacgctt cgacgagctg ggtggtgtaa
     2221 taccccactt gccccgagat gtatgccggc aacgcgatcg ccagcgggac attgttgacg
     2281 atttgatcgg gaacatcggg gttgacttgg tcttctacgg acacatccac cgtgattcga
     2341 acgctgacaa agagaatgaa aaaggccccg acgaccagcg ggaccaagaa gcgccgattg
     2401 aacacaaaat agacgagtcc gaacatcgca aagaccagga gcgcgagctc catgagactt
     2461 tcgaatcgtc cggtggtcat cataatccgg atgcccagca gaatctgggt caacacatcg
     2521 ccattgccga aggtatagat cgtccacatg gtccgctatc ctctcccctc acatcgcaat
     2581 caggcttcct ccgacgacta ccgcagcgat cgcatttcgc cctgcgtctt cgtcaactgc
     2641 atgagctggt tgatgttatt caccatgcgt tgccgctttg cgagactgtc actggccccc
     2701 ctcaaaactt gatgcagccg cccctgaaaa tcttgcagat tgcccggcca ttctggtttg
     2761 accttttgga tatagctgac gttccgggat gtttcggatg ccgcccattt cacccattga
     2821 tatcccatgt ctgcagccac cacttctgat gacaagcgcg caatctgatt gccgacatcc
     2881 cgtggccgcc cataatcagc gagcatggtc agaatcggga ccggtgtcat gttgatgatt
     2941 tgttgatccg ccgcactgat tgcagagccc gagtagaggt cttcgatgag gttgctcatc
     3001 gacgtataca cacgactttc gaatccttcg atatcggtcg catccgtaat cgtggggttc
     3061 aaacattcgg aatcgccccc gcaattaaat attttgacca cgtcccccaa atggctgtcg
     3121 accagctgtt cgaacgtcat cgtcggatcg cgggtttgaa catcgccgtc gttcccgaca
     3181 atgatggtac ctgtgaccga catcaccatg acaatgagat tctgatccag gccggcctgc
     3241 gtcagaacat cccacaccag attacccgtc acatcgtatt ccgtgccctt cagagctgag
     3301 gccgcatccg cattcgactg gttcttgacc gcatcgcggt cctcaaacac gtctccgatg
     3361 gcgccccccg cctccttcat cttctgccag gcatcgccga accgactcgc aagcttctgt
     3421 ttcgaggctt cgttcccggc cagcgcctta cccaattcaa tccccgtttg acagggttgg
     3481 atccgtcccg cggcgttgag tgctctggca gcggcttcga tcttattgag cacgtccgcg
     3541 cagtcttcac agatggcttt catcgccagc tggaacgcgt accccaagac ggccccggtc
     3601 ccgatttgct gcagcaactg gacgtaccga tccagcttgc cgtaggaaaa ggagcccaga
     3661 tacacatcga tgccttggca gcccacgctc aatcgcggag gggcaatcga ccacagccgc
     3721 atcgggtcct gccacaaacg aaccgagacc ccaccgccgc tgaagtaacc tcgacgttga
     3781 ctctcgtagg caccgggagg actcgtgatc ccgacacccc acgagctgaa catgttcgtg
     3841 agggcatcat tcatcgattg cgcctgcacc aggggaggcc aggccagtac ggcccacata
     3901 cacagtccca ccagaatccg tttgcatcgc atcgtgtgtc ctctatccat gagacggtcg
     3961 ctcgcccaac aatcctcgat tccgcaacag gaccagcaat cgctcccgta gatccgcatt
     4021 cgtcatcagc ccggccccga gtggctttaa gaagttttcc tcgggaatcg cgagataaat
     4081 catgggcacc atatgcacgc ccacgttttc cgccatgccg ttgttgatgg cgggttgcgg
     4141 gtactccggc aatcccaccc catccagcga cacaggtttc accgttagtc cgtaggtatc
     4201 ggcgaagacc ttcaatacct tggactgttc ttgacaaaac ggacaggtag aggtgaagaa
     4261 gaagaacagt ccgacccggt cggctgtgcc tgccagcagc tgctcttcgg tgtttcgctt
     4321 gatctcggat tcgacgctat gcccataggt ggacgtggga tgttgcgacg catagtcgag
     4381 cttggggtcc gtgtacatgg cgatctgcca ggcatcggtg aagcgttgcg agcggtcata
     4441 cgcttccttt tggactttga tgtaactcgt cacattctcc accgagggat cgtccaacgc
     4501 gcgttccttt ttggccgaca ccagctcccg caacaccgtc accgggaggg cggttaactt
     4561 gactccatcc atcgcaagcc ccttgaacca cgtccggtat ttcgacggct ccagccaagc
     4621 cccagggggt aagccgtcaa tcgtgacgga tacaggactc tcttccgcgt cctcctcagc
     4681 ctcggctgga gggtcttgat aaaagaacca tccctgcctg ggatgatcga taaacgtctt
     4741 agagccggga ggatcagcgg ccctcgcaac accggtcggc ccccccatcg agcagacgct
     4801 cacagagcac cacgctagag caagcaacag ttttgtcgtt tccatagcag ccacacaaag
     4861 tcctcgccaa cggccggcac gattttgccg agcccccaca tcatgagtgt ccgccccaga
     4921 ggctgacagc acggcggagg aattccggtg ttcggaatag gccacagtaa ttgaaacttg
     4981 tactggcttt tcacaatgag agctgtcgta acgtcgtcac aaagcaacgg gccattggcg
     5041 gacgtccaaa actctgtccc cgcacgggcc agtttggcta ataaccgtgt catcgacagg
     5101 gcagccggct tgaggtctcc atccgctccc gagacatgtc cggtcggcgg atacatgaat
     5161 ccaaagctcc cggcacacca gaacagcgga tcaatcggga agccaaccgc tgcagccacg
     5221 gaatcggcag cacaggctgc taacgacacc ggattggcca ggaccaaggc ctctggaaag
     5281 aggatcagtg aaatcagatc atcgtcccac aagggatcga tctcggaggc atacgccaaa
     5341 tcgatctcgt ctgcgatccc ggtaggattc agacatgcga tgtccaatcc gatgccgacg
     5401 atggcccaca ggggaaaaat gaaataatgc acatgaaaaa atgcgtaatt gagttcggag
     5461 tccatctgcg tcgtagccat gcctcgcccg atgtaactga agcccccggc caaggacacg
     5521 ccgagcgtcg ggaaacacag gggatcgtgt accgcctcga ccagattctt cggttcccag
     5581 atccccagcg gtaacccagg aatgcagata ggcccaagac aaacgcaccc gcacaactgc
     5641 gggggaaaag gggcgcggcc tggaccgacg gccggggcgg taaagtcttc tcccgggtta
     5701 aacaacgtga ctccgctgag agaaatagga aacatacatt cccaacacgt cgtcgcgaga
     5761 ctattcgcca acacagggtt tccgattgtg cacacggcct ctgccgtccc agacggccac
     5821 agcgtcgcca cgccgatcag cacacaactc aggcagacca gcaagaacac ctgccggatc
     5881 cctcgcatcg aggacctagt catgaatcgc cacctcctcc acccgcagat ttcggcctag
     5941 ctgagagacc acactgggca ccgctccgac ccccatgcgg ctgacgagca gaggattcgc
     6001 ccaatagaca cgttgatgga gttgctccat tactttctgc acgtcgccgt cagagatgat
     6061 gagtttcacg aacttagggt catgctccct caaatattgt tgcacccaac ccatctgtgc
     6121 gggactggtg ccgtcaaaaa acacgagggt tcgaatccac cgcaccttgt cgaaggggtt
     6181 gatcttcgtg ccggacttga tcagcaccgt gcccgtggtg ggatccttga tatcctcggg
     6241 gtactcgacg gtcggatcga cgaacgccac tctggcctct tcgacatgcg gtagggccag
     6301 ccctggcccc ttggcgaaat gccgtttgag ggcatcgccg gaacggcgtt gaatgtccgg
     6361 ccaatttgcg ttcgccactc gctccttcat cacatcaatc atgtcccgct ccgcaatgtc
     6421 ggctccacgt gcgtcggccc acaccttcac actccgttcg atggtcggaa gcagtggcgg
     6481 agcttcttca gtcggtcccc cctggagaaa ccctcgatgc cggcgctcga accattcacc
     6541 ccatggcttc aacctcgcct gctcctgtga caacagcgcc aggagatgtg cgaccgagga
     6601 tgccccactc gcaaccatga acttctcgtc cttcacgaac actagggccg gaacctctcc
     6661 tgcaccgact gtccggtaga tcaccggatc gatcccgatt ccgaccagca gggagtcatc
     6721 cgggcgttcc ccggtgaaga gcacccgctt aatccgatcc tgcgtaacct tgaacgactc
     6781 ctgtaccagt ccgcgcagca gcaccttcgc gccaagcagt ttggcttccc gcaggtagtc
     6841 tttgagggtg tcgtccggta acgagaagga gagcagaagg tagagtccgt cgcgacgcat
     6901 ctctttcggc gattcctggg ggcgcccctg ccgtaacgcc tggcgcgagt accttgcgat
     6961 gtcctgagcc atttcctcag gggacggttt actcgggcgg cctgtcgatt cagagagcgc
     7021 gggggatccg atgctcagga gggagagcac tacggtcact gccatacagg cgataacact
     7081 cgatcctttc acggtacctc catatcagaa tgtctgtatg gagtattacc gacctcgcta
     7141 gatgagcggg gtcaccgtac cgagaatatc cgcctgccga atcaggccat agtaacgaga
     7201 gtcaaacgat cgcgggtgtg gcagcgccac atagtattgc ccttcgggaa tagtctccgg
     7261 gagcgtggct aggtcgatcc gttgcccgta gcgatcgatt ccgattcccc gccccaccga
     7321 ttgtccgttg atatagactt cattatcttg gactagaatg tggtcacccg gcactccgac
     7381 tatgcgcttc atccaccgct ttcccacgtc cgcgtagtca cggtgtaagc ggcccggctg
     7441 gacacgttcc gcatagtctt gctgcatgat gaacgccaca agactccctc gccccacgga
     7501 ttgacctttt tgaatgagga agacattatg gtggggccaa ctcccgctca tgcgagcggc
     7561 aacttcatac cagggagcga tgacaccggc agcaatgatc gtcccgcaga acagcatcaa
     7621 ccccacatgc acccaccgat gccgatcaag ccactcgagg attgtcggcg cccgcttgag
     7681 cacgttcagc agcgataatg cattgaatgg cttcatcgat ggagagctcc tttggcccct
     7741 ctttcctcac ccgatcaatc agcgccagct catccggatt cgtcgtaaac aacaactgcg
     7801 tttcccgtgg tacagtcagt ctgcaaatac ctcgcccaaa tggggtgatc atgaaaatct
     7861 ctgagtactc cgtggttttt gtaacagagg acaggagtcg gtattccatc tccgacaaca
     7921 caatgcgccc attgttccga aacgccgcga tgctctcggt cttttgtttg agcagtccca
     7981 caaactccgc attttcgagc aaataacgcc ccacatcccg catcttgtcg tagtagtcgt
     8041 tgatgccctg cgtgatggtg atcatggagc cacccgactt ccggactcgg cgcgcgccgg
     8101 tctcaaaaaa agccgcggta ttacctccct gagacaacaa atcccacgcc tcatccagaa
     8161 tgacgaattt ccgccgtccg cggttctctt ttttgtacat caccgcctga atattcatct
     8221 gcatctgtag gaggatcacc gatcgcaatt gcttctgctc gttcaaccca tcaagctcaa
     8281 gcaccacgaa gtcgttctcg aaattgacgt tgttcgtgcc gttgaagaga tgggagtaca
     8341 tgccgccctt cgcgtaggac gccaacacgc gcgccaggtc tctggcccga ggatccgtca
     8401 tgcccctcaa cactcggtgc accgaatccg gacagccctc acgcccttcc acctcaagca
     8461 cacgcatgat cgcttcttcg atgaacgaga gctcgacatc ggacagcgga cgcgaggggg
     8521 aagccatttg cgcatgcagc gctttgagcg tcgccaattc gtccgcgcgg tcactcggat
     8581 cgtcatcgcc tccagcactg ctcccccagt gcaccagctt gctgaacgga ttgaaacaga
     8641 ggcgttgagc gttggctccg tactggatgt attgcccctt gagcaactcc acaagtttga
     8701 cgtagctgaa gcctgcatcg atgacccaca cctggccacc aatgcccaag tagctcttga
     8761 tccaattgtt catgaagaac gatttacctg cccccgacgt cgccgcgata gccatgttgt
     8821 aattcgtctg ttgattggca aagacatcca tggtgataag ttgacctgac cgagatgtca
     8881 gcagcaccac tggagcgccg ccccccttcc aatccgccac gatagggcaa atatgcgccg
     8941 ggacctcggt gtggtaggtc ttcatgcgtc tgagtccgtc gatgagggct ttgtcatccg
     9001 atggtaagcc gaggggtaac cccgtcatga atattttcca cccgatgaag tgatcttcct
     9061 gcagagtgat atccttggca cgatataggg ctcgaagagt ccctgtctgc tcctccacgt
     9121 gacgcggggt gtccccatac aggacgactt ggaagtagga tccgatgagc tgtcttccgt
     9181 tttccagttg cgcttgcaca ccgttgaaat ggtccaattt gaacctgagg gagggaatca
     9241 gacccagaag aaacccactg gcttgctggt tgattacgac gtgcttcttt cgcaacttgc
     9301 ctttcgcttt gccctgatca aatttcaaga cattcaacgt caggaaaaac ggacaggtga
     9361 tttgatcggt gaaccggacc aggctcccga tcaattcgcg attgttgccg ccatgccact
     9421 gtacaggcca tgactgtacg gtgagcgtct tgagaaactt cccgtccaac tcaatcatct
     9481 tggtgcggag cgtggccgcc gtatcgagac ggaccgcctg atcccgtatc ggcagttgat
     9541 cgctgtacgc cggcggccga ctctcccagc tgtggcccgg gttcagcagc acatgcagca
     9601 cattcaacaa ttgcagtggc cctacccgct caggcagcaa tcccaaggtg ttcaactgtc
     9661 cctcgatgcc cgacaccgct tgatgtatcc gttcatcttc ccacccctgc gcggtcgtct
     9721 cctgggtcgg gaaggccacg gatacataca cggtgaaatc tcgggggcgt aacggtggat
     9781 tggacgtcag ctgtgtgtct cgagcatgca gatagaactc ggcggtcttc cgcgcatcgg
     9841 ccacatagcg ttcccctgcc tgcccccgtt gcgcgagcac aacgtgatgg tccagataag
     9901 gctcgatgat gggagatgca tgcagcataa attgaaacgc ggttccgatc ggcaggtctg
     9961 actcgaagag attcgtcagc ttctgaatag actcttccga caaccccagt agaggcttgc
    10021 tggcaaacac cacaccgatg cgcccatcat ccaagcgata gacctggttg gtgtcatccc
    10081 aagcctcata gggcaaccaa tcactgatgg ccgtccgctc gaccagctgc tttgccttcc
    10141 actcaaggat cgtcgacata ttcgctcctc tggggtcctt ccactactgg ggtggcaact
    10201 ggccatcatc ggtcaggtaa ttggctgctc ccccggactg gggaagctgc ggaaagccct
    10261 gcggcatgat gccgccagaa ccaagaggat tcacgggcat cggcgccgcg cgctgccctg
    10321 cttccggaat gttctccgtc atggcgttca ccgccgcgtt cccactggag ttcctctttc
    10381 cgtctcgttt ctcgaccatc cgccccattc tcggatacaa atcttttcca tgccacgaac
    10441 tcgatccggg actcacaccc tgcgtcatat gcccgaaaat aaagtccggt tcgtcgacga
    10501 tgagatagtg acgcgactga tcgtggaggc gttttttcgt atccttccag ggaaataccg
    10561 taacacggag ggccgaggcc ggcgtgatca ccggcttacc tacagcactg tccggtttgc
    10621 cgctgaagac catcggagac ggggcctgac caggccctga attaccgctg gtgtcgtgtg
    10681 aggcgtcctt ttggtctttc gtgtattgcg tcaccttctg gccggcagcg aagcgcttct
    10741 cgtaggcttc ggagactgat tcacacgtcg cttgatctgg atacccctta caactgaaat
    10801 tggattcata gccgccacag cctgcgaaga ggacacacag gctcatagct gccccagggc
    10861 ccaagccgtg ccatgaaccg ccttgcgtga tacagcgcct cataaaccca gacatcatgt
    10921 ggtctgcctc cttgagaatc cgccccgacc gtattcccta cttctgctcc atctctagct
    10981 gcaatagcgc caacacttca ctggcaggac gataaccggc aaacgattga ccgttcggca
    11041 agaacaccat cggagtggaa tgcaccccca gccgcttccc caatttgatg ttctcgtcga
    11101 tcggatgact gcaagacccc gcttccccca ccaccggctt tgacatcagg gcccgcccca
    11161 gcgcctcagc ttggtcggaa gcacaccaaa tggcgacgga cttgcgatac gcatccggat
    11221 gcgtccgcac gagcggatac agcaccacgg ctaccggaac tcctgcctcg acgagctttt
    11281 gcacctccgg atgaaactgc tggcagaacc tacagtctgg atcgtcgaag accagcagcg
    11341 ggcgatccaa cttcggttgc atcggagaca gtaggatggt cttcgacgca tccatcccgg
    11401 ccagcagcgc ctgttgacga tccctcacat gctcttgcgt cacattccgg cccatctcga
    11461 tatcgaagag tgccccggag agcacgtatc tcccggagtc atgcacgtac atgaagtttt
    11521 ccactcggtc actgtcgctc tctaccaaaa gccaccctgg gatttccatc ttggacactc
    11581 gcagcacttt catgccggga aacttctgtt cgatcaccgt tttgatgggc attactgtat
    11641 catccggttc ggctctcaaa tcaggctcac acaccaccga cacacatatc gcacacaaca
    11701 tgcccgccat tagctgctga taagattgtt tcatcgcaac atgccctctt ttgaattact
    11761 ggaagaaccc ctccaactcg cgtcccgttc ccacgataaa atccacttcc cgctgcgcgt
    11821 cgatttcgat caccgggaaa atggaagccg ccatcatgct gtaatgacga gccaacaact
    11881 ccgcggcgcg accggcgcca ccagccatcc ccgacattgc caccggcccg gccggcggta
    11941 agttgaaggc ttggctcggg ctttgggcaa cgaaaaatcc ggactgatag ggcatgaagg
    12001 cccgcgagat gccggacaca aacccagcaa gcagcgtgcg gcccaacaat gccccttcct
    12061 tcaagaccac tcgcccacgc atcccctgtt tgccatcttc cccactgaca aaccctttca
    12121 attcgatatc gaccgcctcc ccgttctttt tcacgcacga gatggcgttg ccgcgaatca
    12181 ttacgcgttc cgatgacaaa tctcccaagc cttctcccac caggaaacaa tccttcgcat
    12241 caatcctcgc ctcatttgga agcttcatga tgtcgcgcac catcatgagc accgggtagg
    12301 ggctgtttac agcggccccc ccgccctgcg cccccgtcga acgagccggc gcatccatgc
    12361 cggagagtaa cttgaccggg ataatcgtcc ccgtgttcag ccatagtttc ttttcgtgct
    12421 ttttggcgcc aggcactgtc tctggccgaa acacctgaaa cgcctcggtc gccccgcccg
    12481 caaccccagg agcaggaagc gccccgcctg tcggtttcgg ggcgctttgc acgggaatgg
    12541 gccccttcgg cgctggcatg gtcgagacgg gcgtcgcgct cgctccaccc aagggattgg
    12601 cactgagcgg aggaggcagc gggggcaagg cgccactctt cgtcccggct aaggccccac
    12661 caccagaacg cgccaccgac tcaatccgct tttgcagatc gctcaactgt gtcttcattt
    12721 ctgtcttgtc tcgcccatcc tgctccaaca gtctacgcat ttggtcccgc tgctgctcca
    12781 acgcctcaat cctggcccct gcgtccttca tatagatttc tttgtccgtc gcctcacgat
    12841 tgatcgacac ggccttgacg ggagacttgc cacggacaat ccctggttga ttgcctcgga
    12901 attgctgcag caccatgctc aggacgatga atccacccac caggacaact cccgccaaca
    12961 gctttgaagg ggccggtttc tctggccgaa tcgggtcgcc agggttgaga atcgcgctct
    13021 ccgtcggttt aatcgatgcc atcctaggtc tccggtctga ggttagccct ttgcgtctgg
    13081 atccactttc tgctccggct tgggtttggg tggttcagac ggagcgtcat ccagcagata
    13141 caccaccact tcttcgcctg actcgaccac ctcgcgattt aaggcgatcg ctttttgagt
    13201 cccagtcagg aattcctgtt cccgcaacgt gtatttttgt cccgaggtat tcaccaatcg
    13261 atacacaatc acccggtact tcggccctcg atactcgact ccctgcacca ctttcagctc
    13321 aagccacttc ggatatcgtt cttcgttcat cgtcgtctgc cgatacccct tgggcaacgc
    13381 gccacgcgca gccatctgca gtgtgtcgag tagcccatcc acatagtccc cggcttggcg
    13441 ggcaggatcc gaatcatagg gcacagtccc cgcgcgccta tcctcaatat tgactgtctg
    13501 cgcaccgagc gacgcgctca ccacaacttc gaagacgtac gtttgctcac cggccatcac
    13561 gaccagatcg gccgggccgg tcttgacctg gacgatcagg ttgcgccctt ccggtttggc
    13621 cttgagatcc gccctagagc tcgtcgccat ggtgatcggc gcgggaaacg tcagcacgtt
    13681 caaatccctt ccggacaacg tgaggggcgt cgaaccttca tcacccacca ccaccgccgc
    13741 tcctgctgac ccggccctgg ataagcataa cagcaggcca aggacgcaca tcgccatcgc
    13801 ccggtgcttt gtgtctcgat acacgctgga tgtcatacgt ctccccttac ccctttgccg
    13861 gatctgattg accatcctgc agcgaacgat ccaaatgaac cgctgagagc ccgaccaccc
    13921 acggcctccc ttgctcccat cgcatcgaaa tgcggtactc ccgctcctct tcccgaatct
    13981 cactcttccc cacaaatcgg cgctcgatcc cctcgaacac ggccatatcc ccgtccacgc
    14041 ggctggctgt cacgaaaaac acccgactga gattgttggt cttcacgtac acggcttccg
    14101 ctccaagaga ctcggacatc agcccatagt ggctcgatgg aacccactgc aggagctgac
    14161 gatggtgaaa atcaacagag gcgggcgtga cattggcaat ccacggcaac atggccatgg
    14221 caatggactt tttgtaatac tcgtcgccac ccatcgcatt cgcccaatat tcctgggtcg
    14281 ggttgatcgg caccacgtgc accgcgacgt tctgccgaaa gaatccattc accaccaacg
    14341 ccacagcgag aacgatggcc agggccgcga acacaagatt taaaacctgg gcgttatgca
    14401 atttggattt ccacaactcg attgtcatcg tcgttactcc atcatctctc gggtggttcc
    14461 gtgcggtgcc cctgtcagtt gaaacgtggg cacaccagac caataggccc aatggaagag
    14521 gaatccctcg gacttcccgt tcttcatttt cgacaacacc aacgtgctca cgatcccgac
    14581 cacaatcagg taggtcagga acttcgtgac aatgccgaga aagatgcaca caaaaagcac
    14641 ggccaactca tcaatctccc accagaagat ctggggcagg ctatcgagat atcgtggaat
    14701 aaatgtgccg ttcatcagtc cctctcctac tcccttgtga cggcggggcg ctgatcagcc
    14761 ccgccgtcac gacgccttac ctgcaacgcg cgcttacatc agtcccgtgg taatggtcgg
    14821 gatgatgttg gggatataaa aggcggcggc agccacaccg aacccggtga acatgccggc
    14881 ccaacggccc gccatcgccg atcccaagcc gatcgctccc gcgaccaggg ccaacaacgt
    14941 cccaagcgac ccctgcatat agttggtcaa caacgtgacc aaggggccaa acgtcgcatc
    15001 cgcacccgcg aacgcgggca cggccagcac cacaaacgat gccaacacca tgagccccaa
    15061 cagaagactc attcgccaaa tgtctccggc cactttcctt aagccatcgc ccatactcaa
    15121 gcccctcctc tggctcgtcg ttcacagttc agaagactgc ggttgcccaa ccgaatgacc
    15181 gccctcagtt cattccagca ttgtcatgct cctctccagc ctctcattat ggtttccctg
    15241 agtcgtgagt ggcaccagct gacaccacag gtgttaccgg aggtgcttcc gatcgccgct
    15301 caacctcgga ggaatctgca cacctgcctt gcaacagcat catgacctcc acgcgtcgac
    15361 tctccgcgtg gttcggcagg aaacagcagc ctcctcgacc gaccacttcc acctgaaccg
    15421 gccccttgac gcgcgcctgc agcaccttcg ccaccgcctc tgcccgcgcc atcccgagtt
    15481 cctggtttgt ctcccgagat cccgtcggat cagtgtatcc ctccacccgc agatagagga
    15541 tctcctcgat gcgtagttcc gttaagtccc gttgcgcacg ctctgacagg tcggccgacc
    15601 ccattgcgaa cgagaccctt cggtgccgta cctcaggctc gcgacggcac acgaccgtgg
    15661 tcgggcttcc ggtgggacca ggaatcacag ccttgtccgg actgcttccc ggagcgcggg
    15721 acatgtcacc cggacgagct gccagtgtgc ccgacgaaaa cgtcggcaac ccctgtagcc
    15781 gatggatccc tgctttgatc ggtttcggcg ggagatccgc caccgtcggc ttgttaggta
    15841 cctgcgtgag cggcgtcggt tctggacatc ccatcggacg agacagcagc cattgcggac
    15901 cagattggac cgagaccacc gttgtcggaa aggtgtagcc ctgtccgcaa agtcgcgttg
    15961 gatctgacat tgttggcggc acggcaggtc tcgtggtaca gcccccgacg gtcagcagtc
    16021 ccacacaggc cagaccggcc cacgacatgt aagtcggccg tccccctgtc atacttcccc
    16081 ctttcaccat ggctcccgaa tggccaggct catccttggt attaccgact gccacggtgt
    16141 ctcgcggacg aacagaagat gcaggatgtc gaaggggacc tcgtcgacgc gacgaagtag
    16201 gcacgagggg acgcaagcag atcaggaggc gatcagatcg ccaggagcca cgcgtccagc
    16261 ccagactcgg aaggacccgt cggtcgatcg ttggaataac cgagcgcact gaagacactg
    16321 atgaagacag ctaacctctt aacatttgga taatacgttc gagggaaggg atctgacaca
    16381 tactcgccac tgcgacggca atccccaggg tcagggtctg cgtgggcttg ccgtcgtata
    16441 tccacaccgg tatacttgat ccgtgaaacc gctgcgcttt cttgggaact cgctggagcg
    16501 tctccgacag tttccggaag acgctcggca tgatgtgggg taccaactag agcaagtcca
    16561 gcacggcaag cagccaaccg atttcaaacc aatgccgtcc gttggaaagg gtgtcgaaga
    16621 actacgggta tgggatgact ccggcaccta tcgcgtgctc tacgttgctc ggttcgcaga
    16681 cgcggtgtac gtgctccatg cgtttcagaa gaaaagccgg gcgacgagtg cacgcgacct
    16741 tgagcaagcc aggacacggt acgctgaact caagcgaggg aaacatgact aagacgatcc
    16801 acagtttcaa gagtgtgtgg gacgcgttgg ctgatacgcc cgaacaggcg gcgaacttgc
    16861 gtgcgcgcgc ggcactcatg cggcagatca ccaaggtcat cgagacgaac gggtggacgc
    16921 aggcggaagc gggcgccaca tgcggggtga cgcagccgcg gatgaatgat ctgctccgcg
    16981 gacggatctc ccgcttttcc ttagatgcgc tggtgaacat ggcggcggca ctggggcgac
    17041 cggttcgcgt cgaactacag cctcccaagc acaggcgagt gacccggacg cgtcgtttgg
    17101 cctaagcctg gcagcgaaaa gcgtttttat cgtgcgctca tacggacagc cgatcatcgg
    17161 ttcgacgccc gcctcgcgac aataaaaaag cgaggcagaa agggagacca ccatggcaac
    17221 cacactgcat gagaagatca aaaaactggg gacgagacgc caagaaaaga ttcaagcacg
    17281 ggctgcgcaa ctgattgctg aagaacagtc gttacgtgag ctccgggaac cccccggttg
    17341 acgcaatctg acccggtgcg cagggcctca atccgctccc actgtccccc gaaggcactg
    17401 tagatcctcg cggactcata caacatgtgc ggacgaatac acttcccgac cgcagtggac
    17461 atgcggtacg cgatcttcac gataaccgca tccccatact ggctccgctc cagcgcttca
    17521 gccacgatgc cgccaagcag aaagcgttcg cgtatcaatt ctcccgccac atacgtgatc
    17581 gacctggcac actgttcagc ccgttgcacc agcggttctt gcccgctaga acgcatactt
    17641 gacatcctcc ccggccttca ggccggggag gatgtcaagg actcgtgccg tgatattcca
    17701 acctcagggc acggtccagg ctcggagatg ccttgggcgc taccgtgcca gctccgaccg
    17761 cacgcaggct catgggtctg catcgccgta tcactggcca gcgcctccgg ctcatccacc
    17821 accccccccc acgctaatac accatacaga ctgtcgcctc gcgtccacgc gttctgccga
    17881 catattgcat gcgctcctcg accgcaaacg ccccacactc ccgttctgga atgataaaca
    17941 ccacgcgctc atagcgactc ccctgcgcct tgtgcacggt aatgcaatag ctcagccgca
    18001 gctcctctgc cctgagcgca acctcacgcc cggagtccag tcgcaatgcg atcgtgtcac
    18061 ccatactccc cagcacccgc ccttgttccc cattgaccgc tcgctggcct atatcgtatc
    18121 gagtcttggt cacgatgacg cggtcccccg ttcgaaaccc gccatgacac ggcggaccat
    18181 ccggattcat aatctcctgc agccaccggt tcagccgatc cacaccgagt cctcctgcat
    18241 acacaggaga caacgcctgc cactccaccc ctgtgtcttg atagccgcgc actatttttc
    18301 gtaactgata cagcaggagc gcttcggagg cgaagcgaat tacctgaacc ttgaccagca
    18361 aggacccctt ggagccttct tcgcccaacc gcaccaccgg caggactgtc aacaactcag
    18421 ccatcaccgg agtcgctccc accggagcca gttgctgggg atcgccgatc aacacaacct
    18481 gcccccgaca ggcgagcagc aatcgataca aggtgtgcca ggtcaacatg ccggcttcat
    18541 cgaccaagac caggtcggca tccacagtgg acaccgagta gccgccgccc ccatacccca
    18601 gcagcttatg cagcgtcgtg gcgtcctccc cgaggagggt ggccgctttc ccggtcatcg
    18661 ccgtagccgc cacctttccc ccttgctcgc gcacctgggt catgagggat ttcgctaact
    18721 cggtttttcc actcccagca ccgccagtga caatcgaata ccgataccgc agcgatctgg
    18781 cttgcacgct tgaacgtgcg tcaccagagt cctgctgatt cgcccgaatc atggccaggg
    18841 ctttctttcg cgcgaaatac accaccggcg aaaaccacaa gccctgctcg aatttcccca
    18901 caagccccat ctggatgctc accgacggcg ccaccttgtc tgcaggcagt ccgatcagct
    18961 ctgcgcaacg ggccacaaga tgcgcctcag aaatgcctcc gaccccttct tcgtcggctc
    19021 gcgcgagcac ctccatcacc gcggccttat agtgtccgtc actacacaac tcatgcccta
    19081 acacctgcgc ggccgcgacc gtagcctgaa agggaaggcg ccggcggcgg tacagcagaa
    19141 acggatctgc ggagatggcc cgcgccgatc gcagtgtgcc cttcactatc ccacgcgcca
    19201 ctgctttcca ccctgcacaa cacagccatt ccaccagggg gcctaacaat tggcatacct
    19261 cctcgaaccg aagtggtggg gtacgacgca cgccgaggac ccccagcaac tccgacgggc
    19321 tcgcgcaggc ttcaaatcgt cgtgatcgta tcctggagac catcagaacg ggatcggcgc
    19381 cattggctgt cattcgcccc cagcactctc ccacctcacc gaccgaaggc acgggaatac
    19441 cgtccggaac aaacgcccga tgcttgcgac cgatttcgat ccagtcacca gcccctcgtc
    19501 gcgcacgggt ccgactcgtg atcaccccaa ccacctgagg catttcctca gggcagctcg
    19561 ccaccttggc ggagacgtcc ttcccctgca tactcccttg gatcgatccg tagccgttca
    19621 tccttctccc tcccccattc tcagccatca cacactcgta tccacaactc tgtgacatac
    19681 cgttttgctc ccaaaacggt atgtcacagc atcttcacat cagcgaaccg cacaacctct
    19741 tggtctatcg acgcgctaca cgaactcacc tggaggtcct atgctcgctg aactcatcca
    19801 tccactcgct ctcatggaac atcaggaatg ggagtataga cctcggccat cgaacgccgg
    19861 ccccgaccac tgcatccgca aactcacgta ccatgctcga cacgctcccg cgaaaagtcc
    19921 aagcggccgc ttccttgtcg tcctcgacga cagtagttgg catgaagaac tggtattgaa
    19981 ctggatctcc aagagcacgt ttcatctcca cagccaacag cttgaattaa cctgcgcgac
    20041 cacgctcttc cagggtcgac cgttcccaat caaaggtcac attgacggcg tcatcacgga
    20101 cctcttgggg attgatcgcc tcctggagtt gaaggctatc gaacacttca ccttcactcg
    20161 ctttgcagaa gggaattacc cgataaacca cttcgtacag gtggtcttct atctggttgg
    20221 gttggccgca ctgaatcctc acatcgacga agcgctcctc ctcatcaaga acaaaaacca
    20281 gagcgcgttc ctcgaatatc gagtccgcta cgaccgcgac aacgaccgct tgaccgtgct
    20341 cgaaatcaca cacagcacag gcgcgcgcat ctatcccaag caagactttg tgggcttata
    20401 tcaggaggca ctacgtcgat ttgaagaggt ggaacagtat cgggatcacg gcacattgcc
    20461 tgcccgccca tatcccaatg ccgacaacta ccattgcgac tactgtccgt accagttttg
    20521 ctgggaaggg atcgaacagg tccccgttga gggaatcgcg gaactccgca ctgaactggt
    20581 gccagccgtc gaggagtatc tcggcttgca aaccgagctc gcagcactca ctccgaagca
    20641 aaagcggttg gacgcactca aacagcaatt taagttggaa ttgaagacag agggtatcac
    20701 acgcgcggaa gggcatggct actacatcgc atcgtcaccg tatgaacaga gcaaattaga
    20761 tcaatccaaa ctgtccaaag ccgtcattga acgcgcgacc gtgaaagaat cctacagccg
    20821 cttctcagcc ggccgactgt ccaaaggcat cgacaccacc aaatcgaaac gtcggtcccc
    20881 gctcaagaag ccgaaaccga cgactcaaat cgcgtaactg tattcatgcg ctgattcaca
    20941 ttagtaggag gcatcatgtc cgacgcagaa aaacccacgt tcattcccat gaaccgcatc
    21001 aacggcattt ctaaccgacg tcgcatgccc atcatcggca agatccgcct cggcgtgaaa
    21061 gtgagcggcc ccaacggatt ctaccccaag gaaacgcctc attttgtggt ccctgaagag
    21121 gtcgccaagg tgttcggggc gcaacccaac gagctggagg tgatgttcgt ctccaacgat
    21181 cccacggtca attgcccgca ggccctacgc tggtacggag aagccgccgg gctgcagtgc
    21241 attggcaaca atcaggtggc tcgtcgcctg aaccaagaca cctggaaaac cgaatcccgg
    21301 tcgtgtccct gcgaaaaact cgaatccgga gaatgcggcc ctcgcgcgaa tctcatcttc
    21361 atgatcccca aggtcagcag cggcggtctg tttcaaatcg actccagcag tatcaactcg
    21421 atggaaacga ttaactccta tttcgatttc ctcgcgctta cccttggacg tatcgccatg
    21481 atcccgctca aactgcgtcg cgttccagtc accagacgcc gcgaaggccg cccgaccacc
    21541 cactatccgc tcgaattgcg ctatgaagga tcagcggacg agactgccag gtatcaacag
    21601 gagacggaga cggtcctaaa gaagatcaag atgttggaaa tccaagagcc gatcgaaatc
    21661 aacccggtcc tggatccagg agcgcacgtt gtaatggaag aggagctaga ggacgacccc
    21721 ggtcctgtag cagccgaggt tccgggggac actccgtccg agacgcacga ccccacacct
    21781 ccggcgatgt tcacgccctc gacagccgcc gctaaagcgc cggagcccac ccccctgccc
    21841 gctggcccac tcatctcagt ggcccagacc aactacctgc ttcggttgac caaggccttg
    21901 cccctcacag aggatgatct tcgcatgaag gtccgcgcac tcacgcaacg cgaagcgtcg
    21961 gggctcatca agagactcat cgctcaggac tttagctatt tcacgtcgag cccaggcact
    22021 cccccgatca cgcctgcgaa cacaggggcc gtcgccgagt ccatgaccga tcctcagttc
    22081 aagaagatca tgggcctcgt catctcaatg gatatccagg agacacacgt gaaaagtctc
    22141 accagcggat atactaaacg acaggcctcg accctcatcg accagctcgt tggtggagat
    22201 ttttcagcat tccaggagat gaaggaggag gagctgccag tgcttgtcac tccagaatcc
    22261 gcaccagtag aagagaaagt agagttctaa ttcgctggca gacagacacg gagaatatcg
    22321 ggatgactca gggacggctg tgagccgtcc ctgagcacta ctgccacggg cgggcacacg
    22381 tcaagaatcc gctactcatg agccgtaggt gggtgcatgc gctcagaaac cggaaggctc
    22441 acggaggcct ccccaaagcg cagcaactct tcctgggttt ctgggcgcag ggatccaaag
    22501 acttgaagga tattcacaca attcggattg cgtccgaaca gcgatctcgc ggattcagat
    22561 agatacccgg cctcgactag atatgtctca gacgactgtc ctaagacctg ggcgagccgc
    22621 tccacttccg cttggctcaa gcgaccgatc cccgatagca cctgcttgaa tcttccacct
    22681 agctgtacct ggtccgtcac cccagctagc tcctcatccg aatactcact caggtcttgc
    22741 ttaacccgct ccaaaaaggc agccggcatg ccagtaagaa tctgatcatc taagagttct
    22801 gcgacttccg gggaacacac ttcacgggct cgggcgacca acagttggta ggtccgccgt
    22861 tggacatcaa gcggggatcc acccagacac tgcgcaatgc gccgcaacat gtcgtcactg
    22921 ggaagaatct ttttccgccc actgctcttt cccccttcaa taatcgagat atgcgaggcc
    22981 gtacacccca gtttctcagc caactcagtt tgagtcagat tctgggcaat ccgcatccgg
    23041 accaacaagt ccttgaccaa caccatcccc accacactct ccccgtcggg ccccgacggg
    23101 tacccaaccc acgctgatgg agtgattaaa cgcttgttcg caaattatag gtaaacccca
    23161 atttattgtc catacgccta tggaggttac tctaccttcc caatttcaac ctccctcacg
    23221 gcctgcgtat agtcagactg acattcacga ggcagtaaca cattcatggg caaacaaaca
    23281 tttgtttata ttcatcatcc ctcgccgctc cctggaagaa gggactgccg cacgaatgtc
    23341 acaccactta cgcacggaac catcatcggc tccatcagcg ttgtcacgca ccctttcatt
    23401 ttccgagacg catggtctca cacatctacc ccatcggaaa ggacaccatc atgtcgtttc
    23461 gaacgcaagc tcaacaactc gccatcgatt cggacaatga ctcaccgctg ctcaaactgt
    23521 ttcagcagag caagcccatc atccactcga tcgcgaaacg ctactgcaat ttagaccgct
    23581 cctatgaaat tgaagacttg gagcagatcg
//
LOCUS       DAOQEW010000040        23478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_527_length_23478_cov_8.022585, whole genome shotgun sequence.
ACCESSION   DAOQEW010000040 DAOQEW010000000
VERSION     DAOQEW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23478
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_23478_cov_8.022585"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLS35_06485"
     CDS             <1..255
                     /locus_tag="PLS35_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18338.1"
                     /translation="LMFQETSDRGNYQARYILRHPWTGTEDCAAATAYRQQLRERYEQ
                     EAQTLASLTGWNISDIRHTMKLASLPAEKEKKWYQRLWNN"
     gene            complement(337..777)
                     /locus_tag="PLS35_06490"
     CDS             complement(337..777)
                     /locus_tag="PLS35_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18339.1"
                     /translation="MKVGMGLCVIAGILCAAPLVRAETVPLKPGEYQVTAVTESSSGE
                     AGKPDTHTRCVKEEHLANPDAVFNYYALNGFKPNPANKVMNVSMHGGTVSYDIEGLYA
                     MTRVEGTVSTTGFSVVRKATPKGDKALSVTMKVDGKRTGDCAAK"
     gene            1030..1425
                     /locus_tag="PLS35_06495"
     CDS             1030..1425
                     /locus_tag="PLS35_06495"
                     /inference="COORDINATES: protein motif:HMM:NF022666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNI18340.1"
                     /translation="MTGHRRSLIAAVSGLSLLALLSACSFKATFKETTDTTSNITGTT
                     SGRIWWNEDGLLKSEHKVAAFTAYNADNLEADAARGHGEYLASLDALAHLPDAAVFQP
                     TAQAAFSRWSQAGSSSATELVKALQTAHR"
     gene            1488..2342
                     /locus_tag="PLS35_06500"
     CDS             1488..2342
                     /locus_tag="PLS35_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI18341.1"
                     /translation="MNAPRNPLSIHRIFHPTDFSQDSQVAFVHALKLALVFRAELTIM
                     HVDPDVSPEGFEDFPRIRPTLAQWGLLPESSSKSDVTTLGIRIRKVRALAADAKQAIM
                     HHLTATPTDLMVLATHKHEGFAGWIHHAVAEPVSREMHVTTLFVPSNVAGFVSRTTGE
                     TSLHRVLLPISSHPPSQPAIDAATVLVSQLSKPPVTMTLIHAGAEADVETLALPQKAD
                     WSWNQLFGKGDPVEWILAAGAEFDVDVIVMATKGQDSLLDMLRGSTTERVLRGARCPL
                     LAIPAPLI"
     gene            complement(2343..6794)
                     /locus_tag="PLS35_06505"
     CDS             complement(2343..6794)
                     /locus_tag="PLS35_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI18342.1"
                     /translation="MTPVLPFHPIIADWFTAKFAVATDVQQQAWPEIQSGRDLLISAP
                     TGSGKTLAAFLSCIDRLFQQALRCDLRDETQILYVSPLKALSNDVQKNLQQPLLEIGQ
                     AALSSGLLLPDLRVIVRTGDTPMSERQQMLKRPPHILITTPESLFILLTAEKSRAMLK
                     TVRTVIVDEVHAVAPNKRGAHLALSLERVAALAGGAVQRIGLSATQRPIERMGHFLVG
                     NRSSSPQTQDEHSAAGCSGDEMPVANHVAIIDVGHRRDMDLAVEVPKDELGPVATNAI
                     WSDIYDRVAALVEAHRSTLVFVNTRRLAERVSHYLEDRLRHLGEGVVAAHHGSLSREI
                     RLSAEDRLKTGAVRVVVATASLELGIDVGTVDLVCQIGSPRSIATCLQRVGRAGHWVH
                     AIPKGRLFATTRDELIECAALIRSIRAGVLDHIEVPPAPLDVLAQQIVAASATQAWDE
                     TELYKLCCRAMPFRDLTRETFDAVIAMLADGFATSRGRGRAYVHHDRINHQIRGRRGA
                     RLAAITSGGAIPDTANYAVIAEPDGTVVGSVDEDFAVESLAGDIILLGNTSWRIKGVE
                     TGKMRVEDAQGAPPTIPFWRGEAPARTADLSAEVARLRADLDHRLDPNVPAPPPSAPP
                     VQWLKQECGLDQRGAEQAVQYILAGKSVLGTVPTQHTIVAERFFDESGGMQLVIHAPF
                     GGRVNRAWGLALRKRFCVTFDFELQAAATDEGIVLSLGEKHSFPLETVFAFLNVKTLR
                     DVLTQAVLQAPMFMTRWRWNASRALALLRFAGGKRVPPQIQRMRAEDLLAAVFPDAIA
                     CQDNFQGERTERQIPDHPLAQETIRDCLTEAMDIDGLATVLNQIESGAIACVAVDTPM
                     PSAFCHEILNANPYAYLDDAPLEERRARAVDMRRTLPAALAGEMGALDQSAIDQVREE
                     SWPDVRDAEELHDALLTLGWVPCACMPGWDLLVPRLEVAGRVATLWRGDNKQGWVAAE
                     NHQYGGLLFPDTRIDPESAPTHEPLLEREEVLQRIVLGWMESVGPVTAADLAARLGLT
                     VPDVDGAMLRLEAQGHVLRGRFSTQVSHTTDGVVEWCHRRLLARIHRLTVGRLRKEIE
                     PVTAAEFMRFLFQWQHASPGARLHGEAGLLEVAKQLGGFEAAASAWEAQILRVRLAKY
                     QPEWLDRLCLGGALMWGRITPHPRLMQELALLPGRRVVPTRVAPVSLFAREDAPVLLE
                     ATGEDLARLDLSSKLSVSAQAIRHCLQQRGASFFSELLHGARLLASEVEDGLWELVAA
                     GLVTADGFDNLRALIDPRRRRAEASDRNRRPRHVGGRWSLLRPAVGQSQTGPQAATTL
                     RASSSSAHVVGSSERAQATERIARQLLQRYGVVFRDLLARESIVSSWRDLLVCYRRLE
                     STGELRGGRFVSGFTGEQFALPEALEALRALKKRPGTATQQEIKISAADPLNLAGIIL
                     PGPRIAAVPSNFVVFREGVVIRTVTGRSATDRQEPPILEVAQRDLRS"
     gene            complement(7230..7661)
                     /locus_tag="PLS35_06510"
     CDS             complement(7230..7661)
                     /locus_tag="PLS35_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18343.1"
                     /translation="MRKKISRPRVLLGDSSRSMLAFLEILLESHFDVVGSETEGDALV
                     LTAKTLAPELVILDFSLSFLDGLSVGRQLQEAVPDSKVVFFSSHEDAAYVTAAFEVGA
                     MGYLVKHRTVDPGHYLRRVLDGERVCCPDDLHKRVGRPKKK"
     gene            complement(7813..8457)
                     /locus_tag="PLS35_06515"
     CDS             complement(7813..8457)
                     /locus_tag="PLS35_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18344.1"
                     /translation="MTLPRVLLADDHTLVLEGFRRLLDDQCELVGTVGDGRALLEAVP
                     QLKPDIVILDISMPVLNGIDAARVMKVKFPQVKVVFITMHADPAYVRAAFEAGASAYL
                     LKRCVGEELAQAIRAVRSGNFYVTPLVTKEVVESMLRGVDGGAPSGPELTTRQREVLQ
                     LLAEGHTVKDIAGTLKISPRTVEFHKGQIMEQLNLHTTVELVKYALAQGLTSQT"
     gene            complement(8454..9557)
                     /locus_tag="PLS35_06520"
     CDS             complement(8454..9557)
                     /locus_tag="PLS35_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18345.1"
                     /translation="MIGRPTDAQAQRRQRILAGLAGLLACLFFVLDLQLPLGVANAVL
                     YSAVVLLSSASQYRWLPIATATACSLLTIIAIPFSPRIPNLPPWFEWVNHLFSLFGVW
                     APVVFVYQRRRTERLLEEINERLERGVETRTADLAASRLALERSEEQLHTLTGRILTA
                     QEEERRRIAQDLHDDVNQRLALLMLELQSVDRQIGVSPVAAQDGIHNVLKGLEALSDD
                     VRYMAYRFHPSILDDLGLKAALQRLLDDFSTRTGVKALFVHQPLDHPLDKTASTALYR
                     VVQESLSNIARHAKATRVEVEVTVEEEGVVVVVRDDGKGFDQLTVERCEGGLGLLNMR
                     ERLLSVQGTCEVESSPGKGTTVSMYVPLVRVAI"
     gene            9614..10516
                     /locus_tag="PLS35_06525"
     CDS             9614..10516
                     /locus_tag="PLS35_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18346.1"
                     /translation="MRLCPETRTRIGSNRQQQTNRQFGYDARMPSLRPIVILGTGYTG
                     RWIQKLAREQSLPILASSRQPEHHLSDIAQTARIRFDLESPSTWSALPLDADLIWTFP
                     AVPLEQVKAFATHSCSHSRRLVVLGSTSAYDRGDEPLTDMPPWIDETCPVNVDLPRVQ
                     GEEYLRTKHGGIILRVAGIYGPHRNPVEWIRQGRVGPTNKFVNLIHVEDLAALCLLAL
                     QIGRAGDIYNISDGLPRRWTEICVEVSTRWGIVSPREATSSQPGKRINNRKALEGLRY
                     TLRHPDLYRTLATLQQESGVRNCP"
     gene            complement(10524..11837)
                     /locus_tag="PLS35_06530"
     CDS             complement(10524..11837)
                     /locus_tag="PLS35_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNI18347.1"
                     /translation="MKDKATWLTLTIFVSACALLWLTGVTSTKHLVDNLIAGYLLSWG
                     LYAFLSDLPRREIQGRFILMTLCGIVVLGTAELLGVTGLVNYQMLFGTQGREWFDRSG
                     YVRDPELGYRRQAHYQEEGAFVRGNIGEALCLPANAPQRFDLRYDHRGFRNDTDLDRA
                     EVVVIGDSYVESPMLPGDSLLTTQLSHRLRTSVANLGISGYGPEQELVVLKRYALDLQ
                     PKTIVWVFFEGNDLYQLDPEEQNLSAVPDTRAEHDEYWMRSLTRNLLLLSRKAVQGCV
                     PHPDYLRLRGTFHDTEGNETLLYFWERLGELKPTDRLRLERLRSILTEAYHVSRQQGI
                     RLVVAFAPVKHRVHQGLKNFEPLTAEMREWPLNELPDEVASMLNTLSPDIEFIDLTPP
                     LREAAAQGVLTYLPDDTHWTAEGQRVAGEAIHHLLAGGQTRVAKQ"
     gene            12158..12766
                     /locus_tag="PLS35_06535"
     CDS             12158..12766
                     /locus_tag="PLS35_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNI18348.1"
                     /translation="MTATKKTKPLKRASPPATKAEVHVGDIVRRLRKSHHLSVRTLAD
                     KCGFSPSFISQVELRQASPSIASTEKIASALGVTLGEFFRAIDPTPPAIIRSDSRPVV
                     QSEWSRAKIEALGPFNKDSQFEPMVITIQPGGASGHKPYVRQAEQLAVVLMGSLELTL
                     EEDVHQLKRGDAAGIPAGNRHCWRNISKRPAQILIVTAHRRL"
     gene            complement(12853..14346)
                     /locus_tag="PLS35_06540"
     CDS             complement(12853..14346)
                     /locus_tag="PLS35_06540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNI18349.1"
                     /translation="MDADESTGPVSQSSRPPAPCVGAPPPPHAYNPGFSRAVTLLSAV
                     VLSASIAVVAWLSFDIPRVERVADPGRALSHMVGRLMDQADGVKTLPVWEQFLYDLTM
                     GSDANDREQAIEWYRELAEESSDPFVDLHLAILEGEAGHLPEVRQRISRWIRQSAPYP
                     LFARLLQAGYVDAHVARSAGFDLQASLAEQPVSGWFYSRLAARIAERAGDRPLLVTIE
                     TADQQRIETLVWRSRAFALLELGLMVVGGMVLMVWVRRGTGANMFRVGSAELPPLWAG
                     RLGAGVLLRGGAVGALLTVAFLFAAGDVPSLRVVAVPLSNLPLLALAYYHLLRPQRQT
                     FWRGLGLSLEPRHVGQLSLAVLAVVAAGLVGEWVLGRLAEPLNLISHWTEWFDADLVW
                     GTPPTLIVSLMEYVVFAPVFEELAFRGLLFGVFRRRFQWGTAAMLSAALFAIAHGYGL
                     IGFLSVFWSGVIWAWAYERTGSLWPGMIGHAVNNLLVCLSVMALLRP"
     gene            14559..15776
                     /locus_tag="PLS35_06545"
     CDS             14559..15776
                     /locus_tag="PLS35_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNI18350.1"
                     /translation="MSLSSHDHAFVPPHRLLMGPGPSMVHARVLHALSQPLVGHLDPI
                     FLNLMNTIQASLRTLFRTENEFTIALPGTGSAGMEAVIANLIEPGDRAIVGVNGVFGS
                     RLATMIERSGGIPLRIEAPWGQIISLDAVHDALSRGGPVKAVVLVHAETSTGAWQPLE
                     DVGALCRAHGALLIIDAVTSLGGLPVEVDAWGIDACYSGTQKCVSCPPGLAPLTVSPR
                     AMEAVRQRRTPCHSWYLDLSLIAEYWNDRSRAYHHTAPISMLYGLHEALRLIHEEGLS
                     PRFIRHQLNSDALLAGLEPLGLRPLPPAAHRLPMLSCVTLPEGIDDTLVRTQLLQDHG
                     IEIGGGLGPLRGRVWRIGLMGESCQQAHVLTLLNALEDIFAQHGWLDQPGRAVQAAVE
                     TYTQSQLSARRGA"
     gene            15773..16375
                     /locus_tag="PLS35_06550"
     CDS             15773..16375
                     /locus_tag="PLS35_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNI18351.1"
                     /translation="MSRNGFWIVMAGVAGFVAAITYWVIALAVAESRKADMVAPERVT
                     SFIHAVIDANRANYTQNVVDKLHTQGVVEALEHWKEEKGLPLPAQFLLESGRLVAQKD
                     MKLSFRLASLTPIYVWNGPNSEFERRGLEVVMKAPEKPFTGFYQQGGVRYFQGIYADR
                     AVSESCVSCHNGHANSPRRDYKLNDIMGGVIVTIPISEAP"
     gene            16372..17580
                     /locus_tag="PLS35_06555"
     CDS             16372..17580
                     /locus_tag="PLS35_06555"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNI18352.1"
                     /translation="MTIAIRQIRILDGQGRKIERGTVVIEGDRIVAAGPDQTVRLPRG
                     AQRIDGRGLTILPGLIDCHVHFCLGAEADVVAAVEQESSAVTLLKAAELARRTVQAGF
                     TTVRDVGFRDHAVFALKKAIASGLTPGPRILAAGLAICMPGGHARFIGREAQGIEAVR
                     AAVQDQLTAGAEVIKVIASGGVLTPGTSPDEAQMTVEELRAAVEVAAAHGRHVAAHAH
                     GASGMKNALRAGVHSIEHATLMDEEAAGLMTQRGVYMVPTLSALATTAACPTGCGIPD
                     SARSKAKNMVRQHEKSFRAAVRRGVPIALGTDAGTPFNYHGDNAQELERMVTLGMTPM
                     DAIMTSTSAAARLLRLSAEIGTIEAGKQADLLVVEGNPLRNIGLLLKRERIAGVMQGG
                     RFVSGPLSET"
     gene            complement(17671..18309)
                     /locus_tag="PLS35_06560"
     CDS             complement(17671..18309)
                     /locus_tag="PLS35_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNI18353.1"
                     /translation="MPRIDYYRVLGVSRDISDDEIKKAYRKLVFEHHPDRNPNNSKAE
                     EKIREINSAYEVLGDPESRRTYDRLHWGEEMRPETVDPALILEEMEKKLFDEGRKEVF
                     AVLMKMVPRIKSELAIIRERTVAHQGYDTFKEPIVEARGAEVLDEFVTPEMEQRKHRL
                     LEVAVQMMVSQSVVARGDEGGIRALRGRLEEMFRKGRINGFATALELLYQRR"
     gene            18423..19694
                     /locus_tag="PLS35_06565"
     CDS             18423..19694
                     /locus_tag="PLS35_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI18354.1"
                     /translation="MHGVTTYQWLVLFVAWLGWVFDAMDATIYAIVLHPALHELLQAG
                     ATGGEVTSEQIGWYGGIVFSVFLIGWALGGIGFGMAADYFGRTKTLIATILIYAVFTG
                     LAALAQEWWHLAIYRFLTALGVGGEWAAGAAIVAETWPEEKRSRAAGILQSAWAFGFF
                     IAAGCNLLLKDSGWRMLFLIGILPAFVALLVRRWVKEPERWVQAHEREPRSAHTSWLH
                     LVELFRPSLRRDTLIGSTLAFVAVFGVWGATNWAPTLIRGMPDLQGQDSAALAEKVSY
                     AIMALNAGALFGYLGFGPLAERFGRRPVFGLMCLGSLILLPATYFVPHSYAEVLLLLP
                     VLGFFNNGIFSGFPIYLPELYPTRLRATGSGFCFNAGRILASAAPFVTGWLVTMLGSF
                     NNAASTIALIYLLGLAILPFATETKGRPLPE"
     gene            19741..20427
                     /gene="pxpB"
                     /locus_tag="PLS35_06570"
     CDS             19741..20427
                     /gene="pxpB"
                     /locus_tag="PLS35_06570"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNI18355.1"
                     /translation="MIPRMIQLSESAVTVDFGDGIDLHTNELVLAFTAAVDQSKIPGF
                     REVVPTYRSATVYFDPLLTDADTLMKKVRALTVDTVVAPSRASITHTIPVWYGGAAGP
                     DLPDVARQAGLTPEEAGELHASVAYRVCMLGFSPGFPYLSTVPAPLAAPRLPTPRKYV
                     AAGSVGIAGTQTGVYPQASPGGWRIIGRTPVVLFDLHRPKPFLLAPGDQVRFVAIEEE
                     EFTQLAGDQA"
     gene            20424..21200
                     /locus_tag="PLS35_06575"
     CDS             20424..21200
                     /locus_tag="PLS35_06575"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNI18356.1"
                     /translation="MTRLRHIDLNCDVGEAATPEQRDVEARLMPYVTSVNIACGVHAG
                     DAALMRSTIQLARQYDLAIGAHPGLPDRESRGRRELPLSQGFVLDLILSQVSDLMTIG
                     QAEGIRLSHVKPHGALYNMAARDPELADAIAAAIVQLNRRLILIGSAGSELLTSGRAH
                     GLAVAAEGFVDRAYQADGRLVPRTEAHAVLHDESTVLARAHALVHDGTVVAANGTMLH
                     LHIDTLCLHGDTPDALRLAHALRTMFAESGISVRRLDHAD"
     gene            21190..22167
                     /locus_tag="PLS35_06580"
     CDS             21190..22167
                     /locus_tag="PLS35_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HNI18357.1"
                     /translation="MPTKQPLIHVLHPGLFTTVQDLGRSGYQRFGVSVSGAMDPWALM
                     VGNRLLGNPDQAAGLEITLQGPQLLFEQAVRIAITGADLSPTSNGLALPMWTVVAMPA
                     GSRLQFGMRRQGARAYLTVAGGITGPMILGSLSTHVRSGLGGLAGRAVKKWDQLFVGT
                     ASRAAAQPIGRSLPERYRPRYTGSPAVRVMPGPQSDYFTDDAIRLLTSSPYRVTTESD
                     RMGYRLEGAELPHRASAEIVSEAVCVGSLQVPSNRQPILLMADCQPTGGYAKLATMIG
                     ADRSLAAQLAPGDTLSFRMISAEKASELFRSTYAELDRILPPAVLGAKP"
     gene            22288..22488
                     /locus_tag="PLS35_06585"
     CDS             22288..22488
                     /locus_tag="PLS35_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18358.1"
                     /translation="MDTTLTPRQITHDEKKAAEAAFAGRPFNPKWSDAARIVYDGLLA
                     AMGKEARAVDTHDELEPVEPLA"
     gene            complement(22590..>23478)
                     /locus_tag="PLS35_06590"
     CDS             complement(22590..>23478)
                     /locus_tag="PLS35_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18359.1"
                     /translation="RPRNFGQLIDFYGGQRDLKERLIRVLHSLSFVEDPTRVFRAIRF
                     ELRFDFHLSKDTLALIKGAVKMELFHRLSGQRLLDELRLLFSERTPRQAVRRLADLDL
                     LRFIHPTLTWSARLDRRLSELEAALDWYRLSCLDRTIDGWVVYAMALAETMPDEAVRD
                     MLERFPFTEAERTAINQARFLTQPVCRTLARRAPLRPSETVAALAGFSEECLVFLLAK
                     NGSDTAKRSLSQYLTTYRDIKPALTGKALQALGLKPGPVYRTILERLTEARLNGEVHT
                     EEEERALVKDLIARRRSAS"
BASE COUNT         4859 a         7501 c         6667 g         4451 t
ORIGIN      
        1 ctgatgtttc aggaaacctc ggatcgtggc aactatcagg cgcgctacat cctgcgccat
       61 ccgtggaccg gcacagaaga ctgtgcggct gccaccgcgt accgccaaca attacgcgag
      121 cgatacgagc aggaagcgca gacactcgcc tcgctgacgg ggtggaatat cagcgatatc
      181 cgccacacca tgaaattggc ttctcttccg gccgagaagg agaagaagtg gtatcagcgt
      241 ttgtggaaca actagcagcg agagaaaccg gaggccggaa tgccggcctc cggcatgcca
      301 ccttcgctgt atgcagattc gatatcgggc aggaacttac ttcgccgcgc aatcgcctgt
      361 gcgtttgcca tcgaccttca tcgtcaccga cagggccttg tcgcccttag gggtcgcttt
      421 gcggaccaca gaaaaaccgg tggtcgagac cgtgccttcg acgcgggtca tcgcatagag
      481 gccttcaata tcgtaactga ccgtcccgcc atgcatcgac acgttcatga ccttgttggc
      541 cggattgggt ttgaatccgt ttaaggcata ataattaaac accgcatccg ggttcgccag
      601 atgttcttcc ttcacacatc gggtatgggt gtccggcttg ccggcttccc cgctgctgct
      661 ctcggtcacg gctgtcacct gatactcacc cggcttcagc ggaaccgtct cggcccgcac
      721 caatggcgct gcacagagga ttcctgcgat cacacacaag cccatgccta ccttcatcgt
      781 cgcccctcct tgtgataccg gggtgcgtct gctcctggct catcacagcg accaggccgc
      841 ctcgcccgaa ttcgtgcccc atcaaggacc cggcgattgt gctgatgcct ggggatattg
      901 tcaaccgtta cctccttcga acagactcaa cctctgacga ccaagggccg gcctaggctc
      961 atttccgtgt cgactatgct atagtcggcc accatcactc catgtggccg catgaaagga
     1021 gcactacgta tgactggaca ccgtcgttcg ctcatcgccg cagtcagcgg actttcactc
     1081 ctggcgttgc tctcagcctg ttcgttcaag gccaccttta aagagaccac cgacaccaca
     1141 tcgaatatca ccggcaccac gtccgggcgc atctggtgga atgaagacgg cctgctgaaa
     1201 tctgagcaca aggtagccgc cttcaccgcg tataatgcag acaatttgga agctgatgcg
     1261 gcgcggggtc acggcgaata tctggcgtcg ttggacgccc tcgcccatct accggatgcg
     1321 gctgtgtttc aaccgacggc gcaggctgcg ttcagccgct ggagccaggc cggctcttcg
     1381 tcagccaccg agctggtcaa ggctttacaa accgcgcacc gctgacgaca cagaccgatc
     1441 accgggcagc acaggcagat tgcacctcac cggagttcga tacggacatg aacgcgccgc
     1501 gcaatcccct ctcgatccat cggatctttc accccacgga tttttcgcag gacagtcagg
     1561 tggcattcgt ccacgcgctc aaactggcgt tggtcttccg cgccgagctg accatcatgc
     1621 atgtggatcc cgacgtctcg ccggaagggt tcgaggattt tccccgcatc cgtccgaccc
     1681 tcgcgcagtg gggactcctt ccggaatcca gttccaaaag cgatgtgacc acgcttggca
     1741 tccggatcag aaaagttcgc gccctggcgg ccgacgccaa gcaggccatc atgcatcatt
     1801 tgaccgcgac accaaccgat ctcatggttc tggcgacgca caagcacgaa gggtttgccg
     1861 gatggattca tcacgcggtc gctgagcccg tttcgcgcga aatgcatgtc accacgctgt
     1921 tcgtcccatc caacgtcgcg ggatttgtct ctcgcaccac cggcgaaacc tcactgcatc
     1981 gcgtgctgct gccgatctcg agccatcccc cctctcaacc cgccatcgat gcggcgaccg
     2041 tgctggtgtc ccaattgagc aagccaccgg tcacgatgac gcttatccat gcaggcgcgg
     2101 aagccgatgt tgagacactg gcgcttccgc agaaggcgga ctggtcctgg aatcagttgt
     2161 ttggaaaggg cgatcccgtc gaatggattc tcgcggcagg tgccgaattc gatgtggatg
     2221 tgattgtgat ggcaaccaaa gggcaggaca gcttgctcga catgttacgc ggcagcacca
     2281 cggaacgggt cttgcgcgga gcgcgatgcc cgttgctcgc catccccgcg ccgcttatct
     2341 gatcaggatc gcagatcccg ttgtgccact tcgaggatgg gcggctcctg ccgatcagtc
     2401 gcacttctcc cggtcacggt gcgaatgacg acgccctcgc gaaacaccac aaagttcgac
     2461 gggaccgctg cgatacgagg tcccggcaga atgatcccgg ccaggttcaa cggatcggcg
     2521 gccgaaattt tgatctcctg ctgggtggcg gtgcctggac gctttttcaa tgcccgcaat
     2581 gcttccagcg cctcgggcaa ggcgaactgt tctcccgtga acccgctgac gaaccgcccc
     2641 ccgcgtagct cccccgtcga ttccaggcgc cggtaacaga ccaacagatc ccgccaggaa
     2701 gagacgatcg attcgcgcgc caacagatcc cggaatacca cgccatatcg ctgcagcagt
     2761 tgccgcgcaa tacgttcggt ggcctgcgcc ctttctgagg aacccacaac atgggccgac
     2821 gaagacgatg ctcgcaaggt cgtcgcggcc tgaggaccgg tctgtgattg tccgacggcc
     2881 ggcctcaaaa gcgaccagcg tccaccgaca tggcgaggcc ggcgattccg atcggacgcc
     2941 tccgcccgac ggcggcgcgg atcgatgagc gctcgcagat tgtcgaaccc gtcagccgtg
     3001 accaggccgg ccgcgaccaa ttcccagagc ccatcctcca cttccgatgc caacaaacgg
     3061 gcaccatgca gcagctcgct gaaaaagctc gcaccacgct gctgcagaca atggcgaatg
     3121 gcctgagccg agacgctgag tttggaggac agatccaatc gggccaaatc ctcgccagtc
     3181 gcctccaaca gcaccggagc atcttctcgc gcaaacagac tgaccggcgc gacccgcgtc
     3241 ggaaccacac gccgccccgg caacagggcg agctcctgca tgagccgcgg atgcggcgtg
     3301 atacgccccc acatcagcgc gccgcccaaa cagagccggt ccagccattc cggttgatac
     3361 ttggccaaac gaacccggag aatctgcgcc tcccaggccg atgcggcagc ttcaaaccct
     3421 ccgagctgct tcgccacctc caagagtccg gcctctccat gaagacgcgc ccccggtgac
     3481 gcgtgctgcc actggaataa aaaccgcatg aactcggcgg cagtgacagg ttcaatctcc
     3541 ttgcgtaacc gtccgaccgt gaggcggtga attctggcca acagccgccg atgacaccat
     3601 tccaccaccc catccgtggt gtgggacacc tgcgttgaaa accgccctcg aagcacgtgg
     3661 ccctgcgctt cgagtcgcaa catcgcgccg tcgacatccg gcaccgtcag gcccagacgc
     3721 gcagccagat cagcggccgt aaccggcccc acactttcca tccagcctaa gacaatgcgc
     3781 tgcaacacct cttcccgttc cagcaaaggc tcatgagtcg gtgccgattc cggatcgatc
     3841 cgggtatccg gaaagagcag gcccccgtac tgatgattct cggcggccac ccatccctgt
     3901 ttgttatcac cccgccacag cgtggcgacc cggcctgcga cctcgagcct cggcacgagc
     3961 aagtcccatc ccggcataca ggcgcagggc acccagccga gcgtcaacag cgcatcgtgc
     4021 aattcctctg catcacgcac atcaggccag gactcttctc tgacctgatc gatggccgac
     4081 tggtccaacg cgcccatctc cccggccagt gcagccggaa gcgtgcgccg catatccacg
     4141 gctcgtgccc gccgctcttc gagcggcgca tcgtcgagat acgcataagg gttggcattc
     4201 aaaatctcgt ggcaaaaagc ggacggcatc ggcgtatcga cagcgacgca ggcaatcgcg
     4261 ccggattcga tttggttcag caccgttgcc aacccgtcaa tgtccatcgc ttccgtcagg
     4321 caatcccgga tcgtctcttg cgccaacggg tggtcaggaa tctggcgctc cgtgcgttct
     4381 ccttggaaat tgtcctggca ggcgatggca tccgggaaca cggcggcgag gagatcttcg
     4441 gcgcgcatac gttgaatctg aggcggcacc cgcttccctc cggcaaatcg gagcagcgcc
     4501 agggctcggg acgcattcca tcgccaccgt gtcatgaaca tcggcgcctg cagcacggcc
     4561 tgagtcaaca cgtcgcgcag agtcttgacg ttcagaaagg cgaaaaccgt ctcgagcgga
     4621 aaactgtgct tttcgcccag cgacagtacg atcccctcat ccgtcgccgc ggcttgcagt
     4681 tcaaaatcga aggtcacgca aaagcgtttc cgcagggcca gcccccaggc gcggttcacc
     4741 ctgccgccga acggggcatg gatgaccagc tgcatccccc cgctttcatc gaagaatcgt
     4801 tccgccacga tcgtgtgttg cgtgggcacc gtccccagaa cggatttgcc ggccaggata
     4861 tattgaaccg cttgctccgc cccccgctga tcgagaccgc attcctgttt gagccattgc
     4921 accggcggcg cggaaggagg aggcgccggc acattcggat cgagacgatg gtcgagatcg
     4981 gctctgaggc gcgcaacttc tgccgacaag tccgccgtgc gtgccggcgc ttccccgcgc
     5041 cagaacggaa tggtcggcgg tgcaccctgc gcatcttcga cacgcatctt gccggtttcg
     5101 acgcccttga tgcgccagga cgtgttcccc aatagaataa tgtctcccgc cagactctcg
     5161 acggcgaagt cctcgtcgac ggatcccacc accgtgccgt ccggttcggc aatgactgcg
     5221 tagtttgctg tgtcgggaat ggcgccgccc gaagtaatag cggccaatct ggccccgcgg
     5281 cgcccgcgaa tctgatggtt gatgcggtcg tgatgcacat aggctcggcc ccggccccga
     5341 ctggtcgcaa atccgtcggc cagcatggca atcaccgcgt cgaaggtctc gcgggtgaga
     5401 tcacgaaagg gcatggcccg gcaacacaac ttatacagct ccgtctcatc ccaggcctga
     5461 gtggccgatg ccgctacgat ctgttgcgcc aggacatcaa gtggtgcggg cggcacctca
     5521 atgtggtcta gcacaccggc tctgatcgaa cggatcaacg cggcacattc gatcaactca
     5581 tcacgcgtcg tggcaaagag gcggcccttc gggatggcat gaacccaatg gccggcacgg
     5641 ccgacacgct gcaagcacgt cgcgatggag cgaggcgatc cgatctggca aacgagatcc
     5701 accgtcccga catcgatgcc gagttccaga gaggccgtcg cgacaaccac tcgcacggct
     5761 ccggttttta accggtcctc cgcagacagg cggatctccc gcgacaggct tccgtgatgg
     5821 gctgccacca caccttcgcc caggtgacgc aaccgatctt ccaagtagtg cgagacccgc
     5881 tcggccaagc gccgcgtatt cacgaagacc agtgtggaac gatgcgcttc caccagtgcg
     5941 gcaacccgat cgtagatatc gctccagatc gcattggtcg cgacgggccc gagttcgtcc
     6001 ttcggaactt cgaccgccaa gtccatgtcg cggcgatggc cgacatcgat gatcgccaca
     6061 tggttcgcca ccggcatctc gtcgcctgaa cacccggctg cggaatgctc atcctgcgtc
     6121 tgtggggagg acgaccggtt ccccactaag aagtgcccca tccgttcaat cggacgttgg
     6181 gtggcggaga gaccgatccg ttgaacggca ccaccggcca gcgccgccac gcgctccaat
     6241 gacagggcca ggtgcgcccc tcgcttgttc ggggcgacgg catgaacctc gtccacaatg
     6301 accgttcgga cggtcttcaa catcgcccgg cttttttcag cggtcagcaa aataaacagg
     6361 gattcaggcg tggtaatcag aatatgcgga ggacgcttga gcatttgctg ccgctcgctc
     6421 atcggtgtgt cgccggtccg tacgatgacc cggaggtctg gcagcaacag tcccgacgag
     6481 agggccgcct gcccgatctc cagaagcggt tgttgaaggt ttttttgcac atcattgctg
     6541 agcgctttga gcggcgagac gtagaggatt tgtgtttcat cccgcaggtc acaacgcagc
     6601 gcctgctgaa atagccgatc gatacaggac agaaatgctg cgagggtctt tcccgatccg
     6661 gtgggggcgg aaatcagcag atcacggccc gactgaatct ccggccaggc ctgctgctga
     6721 acgtcagtcg cgaccgcaaa tttggccgtg aaccagtcgg cgatgatagg atgaaaggga
     6781 agaaccggcg tcatcgggcg atcgtaacac aaccgtcacg agctgaacat gcggtcaaac
     6841 cgttgagatt tgaaatggcc ggaaggaaga cgacgggggc gggacggtac aaacggggac
     6901 cgtgcggggg agcaccgggg tcaccccttg aggcttcgtc ggcccgatct aagccggatc
     6961 atcagcaggc cggccgtctc actgctcgcc ataagatttt aagatctcat acgacaagcg
     7021 gcattcgctg cagtaatttc tcgaaaaccg cacctgggac ggcgacacca catgccgcgc
     7081 caggtaactc gggaaatcga cccaacaatt cgttcccctg gcggaaaatc catcgtcacg
     7141 aacccgacgg cacaactcac acatcggtac gactcggacc atcgtcggtc tccttttgcg
     7201 cgaagctgac tgcatgcatg gagtcggcac tacttttttt tggggcgccc cacccgcttg
     7261 tgtaaatcgt cgggacaaca gacccgctcc ccgtccagca cccggcgcag ataatggccg
     7321 gggtccaccg tccggtgttt gaccagatag cccatcgcgc cgacctcaaa ggcggccgtc
     7381 acataggcgg catcttcgtg agacgaaaaa aacacgacct tgctgtccgg cacagcctcc
     7441 tgaagctgcc ggccgaccga caaaccatcg aggaacgaga gggaaaaatc caggatgact
     7501 aactcagggg caagtgtttt tgccgtcagg accaaggcgt cgccttcggt ttccgagccg
     7561 acgacatcga aatgagactc caggagaatc tcgagaaagg ccaacatgga acgtgaggaa
     7621 tcacctaaca gcacacgggg acgggaaatc ttttttctca taatcctacg agtctctgct
     7681 tagaaaaggg atacgtatag taggcaagtg aagggtaaac cacacgcgta acaaagcgag
     7741 gttttacatc aaaaatatac tagcttaaat actggtagca gagaaagacg ggggcggaaa
     7801 ccgagcagga gatcaagttt ggctggtgag accctgcgcg agggcgtatt tcacgagttc
     7861 cactgtggta tgcaggttga gctgttccat gatttgcccc ttgtgaaact caaccgtgcg
     7921 aggagaaatc ttgagcgtgc cggcaatgtc cttcaccgtg tggccttcgg ccaggagttg
     7981 caacacttcg cgttgacgag tggtgagctc agggccggat ggagctccgc cgtccacgcc
     8041 gcggagcatg ctttcgacaa cttccttcgt gaccagaggc gtcacataga aattgccgct
     8101 ccgcacagcc cggatcgcct gggccaattc ctctcccaca cagcgcttca gcaggtaggc
     8161 cgaggcgccg gcctcaaacg ctgcccgcac ataggccgga tcggcatgca tggtgataaa
     8221 caccactttg acctgtggaa acttgacctt catcacacgg gcggcatcga tgccattcag
     8281 caccggcatc gagatgtcca aaatgacgat gtccggcttc aactgcggca ccgcctccag
     8341 cagcgcccgg ccatccccga cagtgccgac cagttcacac tgatcatcca acaaccgccg
     8401 aaatccctcg aggacgaggg tatggtcatc tgccaataac acacgcggaa gcgtcatatt
     8461 gccacccgta caagcggtac gtacatggat acggttgttc ctttcccggg gctggattcg
     8521 acttcgcacg tgccttgtac agacaagagg cgttcgcgca tgttcaacag acccaacccg
     8581 ccctcacatc gttctaccgt cagctgatca aatcccttcc catcatcgcg taccactacg
     8641 acgaccccct cctcttcgac cgtcacctcc acttccaccc gagtcgcttt cgcgtgtcgg
     8701 gcgatattgg acaggctctc ctgaacgacg cgatacagcg ccgtcgaagc agttttgtcg
     8761 agggggtggt caagaggttg atggacgaac agggctttga cgcccgttcg agtcgaaaaa
     8821 tcatccaaga ggcgctgtaa cgccgccttc agacccaggt catcaagaat ggatggatga
     8881 aagcgatacg ccatgtagcg gacgtcgtcg gacagagcct cgaggccctt caacacattg
     8941 tgtatcccgt cctgggctgc cacgggtgat actccaatct gccggtcaac gctctgaagc
     9001 tccaacatga gcagcgccag ccgctgattg acatcatcat gcaggtcctg ggcaatccgc
     9061 cggcgttctt cttcctgggc cgtgagaatc cggccggtca acgtatgcaa ctgttcttcg
     9121 ctccgctcca gcgccagccg gctggcggcc agatcggccg tccgcgtttc tacccctcgt
     9181 tccagccgct cgttgatttc ttcgagcagt cgctccgtac ggcgacgctg atagacaaac
     9241 accacaggag cccatacgcc gaagagactg aacaggtgat tgacccattc aaaccagggg
     9301 ggaagattgg ggatccgcgg actgaacgga atggcaatga tcgtgagcaa tgagcacgcg
     9361 gtggcagtgg caatcggcaa ccatcgatat tgactggcag acgacaggag cacgaccgcg
     9421 ctgtagagta ccgcgttggc cacacccagc ggcaactgaa gatccagcac aaaaaagaga
     9481 caggccagca ggccggcaag tccggccagg atccgctgcc gccgctgcgc ctgcgcatcg
     9541 gtcggtcggc cgatcatgtc acccgtctcg tcctgacgta agttgaaaga cgccatcggg
     9601 gccggatttt agcatgcgac tctgtcccga gactagaacc agaataggct caaaccggca
     9661 gcagcaaacg aaccggcagt tcgggtatga tgcccggatg ccctcgctcc gccccatagt
     9721 gattctcgga accggctaca ccggccgatg gattcagaag ctggcccgcg aacaatcgct
     9781 gcccattctg gccagcagcc gccagccgga gcaccacctc agcgacatag cccagactgc
     9841 ccgcatccgg ttcgatctcg agagtccttc cacatggtct gcgttgccgt tggacgcgga
     9901 cctgatctgg acgtttccgg cagttcctct tgagcaggtg aaggccttcg ccacacactc
     9961 ctgcagccat tcgcgacgct tggtggtgtt aggcagtacg tccgcctacg atcgaggaga
    10021 tgagcctttg acagacatgc ccccatggat cgacgagacg tgccccgtca atgtcgacct
    10081 tccacgggtc caaggggagg aatacctccg cacgaagcat ggtgggatca ttctccgcgt
    10141 ggccggaatc tatggccccc atcgcaatcc ggtggagtgg atccgccaag ggcgcgtcgg
    10201 ccccacgaat aaatttgtga acttgattca tgtcgaagat cttgccgcgc tctgccttct
    10261 ggccctgcaa atcggacgcg ccggagacat ctacaatatc agcgacgggc ttccgcgccg
    10321 ctggaccgag atttgcgtag aagtgtcgac cagatggggc attgtgagcc cgcgggaggc
    10381 cacctccagc caaccgggca agcggattaa caatcgcaaa gcgctcgaag gcttacgcta
    10441 caccctccgg catccggatc tctatcgcac actcgcgacc ctccagcagg aatcaggcgt
    10501 caggaactgc ccctagccac gccttactgc ttagccacgc gagtctggcc gccagcaagg
    10561 agatgatgga tcgcttcacc ggcgactcgc tgcccctccg ccgtccaatg agtgtcatcg
    10621 ggaagatagg tcaacacacc ctgtgcagca gcctcgcgaa gcggcggagt caaatcgatg
    10681 aattcgatat ccggggagag tgtgttcagc atcgacgcca cttcatcggg cagttcattg
    10741 agcggccact ctctcatttc cgcggtgagt ggctcgaagt ttttcaatcc ctgatgcacc
    10801 cgatgcttca ccggcgcaaa cgccaccacg aggcgaatac cttgctgacg actcacgtga
    10861 tacgcctcgg tcaaaattga tcgcagccgc tcgaggcgga gtctgtccgt cggttttaat
    10921 tcgccgagcc tttcccagaa atacaacagg gtttcattgc cctctgtatc gtgaaacgtt
    10981 ccgcgaagtc gcaagtaatc tggatggggc acacatccct gcaccgcttt acgtgaaagg
    11041 agcagcaggt tcctcgtcaa cgagcgcatc caatactcgt cgtgttcggc cctggtgtcg
    11101 ggcacggcgc tcaggttttg ctcttccgga tccaattgat agagatcgtt tccctcaaaa
    11161 aatacccaca cgatcgtctt cggctgaagg tccagcgcat accgcttcag cacgacgagt
    11221 tcctgctccg gcccatatcc gctaatccct aaattcgcga cagacgtgcg cagccgatgg
    11281 cttaattgcg tcgtcagaag actgtcgcca gggagcatgg gcgattccac ataggaatca
    11341 ccaatgacca ccacctccgc ccgatccaga tccgtatcgt tgcgaaaccc ccgatggtca
    11401 taccggagat caaatcgttg aggtgcattc gccggaagac agagcgcttc accgatattt
    11461 cctcgcacaa acgccccttc ctcctggtaa tgcgcctgac ggcgatatcc aagttccgga
    11521 tcacggacat atccggagcg atcgaaccat tcacgcccct gggtcccaaa cagcatttgg
    11581 taattcacga gtcccgtcac gccgagcaat tcggctgtcc ccaacaccac aatcccgcag
    11641 agggtcatca aaatgaaccg tccctgaatc tccctgcgcg gcagatcgga gagaaaagca
    11701 tacaagcccc aagacaacaa atagcccgca atgaggttat ccacgagatg cttggtgctg
    11761 gtcacgcctg tcagccataa cagggcacat gcacttacaa atatcgtcaa cgtcaaccat
    11821 gtggccttgt ctttcaagct ccctccccac atcgcatctg gatgtgtcac tggtcctggt
    11881 tcagatcagg ccatcagtga ctgacgaacg agcaacgaac actccacaat aatggctgaa
    11941 cgactgtcca gctatactgg acacttcctt agcccgatta cgaaggtctg ggaagctggg
    12001 gaagtcccta ggacgcggtg tcgagaaatc gacagacggc agtagcgcag aggacggggg
    12061 aagagactca tccttgagac tccccacaaa aacgtgttac acaggtaaaa agccggcctt
    12121 tcgcactcgg ctctatgtat gtgttcttaa cggacaaatg acggctacca agaaaacgaa
    12181 gccactgaaa cgcgcctcac ctcctgccac caaagccgag gtgcacgtcg gcgacattgt
    12241 ccggcggctt cgcaaatctc atcatctttc agtgcgaaca ctcgccgaca agtgcgggtt
    12301 ctctcccagc ttcatctctc aggtcgaact acgccaggct tcgccgtcca ttgcctccac
    12361 cgaaaagatc gcctccgcat tgggcgtcac cctgggagag ttctttcgcg ccatcgaccc
    12421 gacgcctccg gccatcatcc ggtccgattc tcggccggtc gtccaaagcg agtggtctcg
    12481 tgcaaaaatc gaagcgctcg gcccgttcaa caaggacagc cagttcgagc ctatggtgat
    12541 cacgattcag ccggggggag ccagcggtca taaaccctac gtccggcagg cagaacaact
    12601 cgcggtcgtc ctgatgggat ctctcgaatt gacgctggag gaggacgtcc accagctcaa
    12661 gcgcggcgat gccgccggga taccggcagg caaccggcac tgctggcgca acatcagcaa
    12721 gcggccggca caaattctga tcgtgacggc tcaccggcgg ctgtgaccgt tgttatcacc
    12781 tttccaccac gtaccagtcc gcacatctac ccatcggaat aaccatgcag tggtctcgct
    12841 cgaacaccgc tttcagggcc gcaacagcgc catcacgctg aggcagacca ggaggttgtt
    12901 gacggcatgg ccgatcatgc ccggccacaa actccccgtt cgctcataag cccacgccca
    12961 gatcacgccg ctccagaaga cgctcaagaa tccgatcaat ccatagccgt gagcaatggc
    13021 aaacaaggcc gcgctcagca tggctgctgt gccccattgg aaccgccgcc ggaacacgcc
    13081 gaataacagt ccacggaagg ccaactcttc aaagaccggc gcaaagacca catattccat
    13141 caaactgacg atcagagtcg gaggcgtgcc ccatacgaga tccgcatcga accactcggt
    13201 ccagtgacta attaaattca gtggctcggc aaggcggccc aacacccact ctcccacaag
    13261 tccggctgcc acgactgcca agacggccaa actcagttgt cccacatgac gcggctcaag
    13321 actcaacccc agtcctcgcc agaatgtctg ccgctggggc cgaagcaagt gataataggc
    13381 caatgccagg agcggcaggt tcgacagcgg caccgcaacg acccgcaagg agggcacgtc
    13441 gccggcggca aacagaaacg ccaccgtcaa caacgcacca acggcacccc cgcgcagcaa
    13501 cacaccggcc cccagacggc ctgcccacag gggcggcaac tcggcggatc cgacccgaaa
    13561 catgttcgcg cctgtacctc gccgcaccca taccatcagg accattcccc cgacgaccat
    13621 gaggcccaac tcgagcaggg cgaacgcccg tgagcgccag accaatgttt cgatgcgttg
    13681 ctggtcggct gtctcaatcg tgacgagcag cggacgatcc cctgcgcgtt cggcaatacg
    13741 agccgccagc cggctgtaga accagccgga aacgggttgc tctgccaaag aggcttgcag
    13801 atcgaacccc gcgctgcgcg ccacatgcgc atcgacgtat ccagcctgta acagtcgcgc
    13861 aaacaacgga tacggtgcgg actgcctgat ccatcgcgag atacgttgcc gaacctccgg
    13921 aagatgcccc gcctcgccct ccaggatggc caggtgcaaa tcgacaaacg gatcggacga
    13981 ttcttcggcc agttcgcgat accactcgat ggcctgctca cgatcgttcg catcgcttcc
    14041 catcgtgagg tcatagagga actgttccca caccggcagc gtcttcacgc cgtctgcctg
    14101 atccatcaga cgaccgacca tatgactcag cgcccggccg ggatccgcga cccgctccac
    14161 tctcgggata tcgaacgata accaggccac aaccgcgatc gaagccgaca gcaccacggc
    14221 agataacaac gtgaccgcac gggaaaaccc gggattgtac gcatgcggcg gaggaggcgc
    14281 cccgacacag ggcgctggag ggcgagatga ttgcgagacc ggaccagtcg attcgtcagc
    14341 gtccacgaga cctccaaggg taaggcgcgc cggagtatac caccggcctt atggcggtga
    14401 aactgcagag catcccgcaa cagagggtgc cgggatctcg ccacaatctc atggctgaac
    14461 cccctctgga ttcgctatac tacgccactc gctgcgccag gcgttgctcc cgagacggtg
    14521 cgaccttccg tggcgagcgg acacgatcag cgtcaaccat gagcctttcc tcgcacgatc
    14581 acgcattcgt ccccccgcat cgattactga tgggccccgg cccgagcatg gtgcatgcgc
    14641 gcgtgcttca tgccctgtcg cagccactgg tcggccatct ggatccgatc ttcctgaatc
    14701 tgatgaatac cattcaagcc tcgctccgca ccctgttccg gacggagaac gagttcacca
    14761 tcgcgctccc gggcacggga tcggcaggca tggaggccgt gatcgccaat ctcatcgagc
    14821 cgggcgaccg cgccatcgta ggggtcaacg gcgtgttcgg ttcacgactg gccaccatga
    14881 ttgaacggag cggcggtatc ccgctacgta tcgaggcgcc ctggggacag atcatttcgt
    14941 tggatgccgt gcacgatgcc ttgtctcgag gcggcccggt caaggcggtg gtactggtgc
    15001 atgcggaaac ctcaaccggc gcctggcaac cgctcgaaga cgtcggcgcc ctttgtcggg
    15061 cgcacggcgc cttattgatc atcgatgccg tcacatccct cggaggcctc cccgtcgagg
    15121 ttgacgcatg gggcattgat gcctgctaca gcggcacgca aaaatgcgtg agctgtccgc
    15181 ctggcctggc cccgttgacg gtgagccccc gagccatgga ggccgttcga cagcgacgga
    15241 caccctgtca cagttggtac cttgacctct cattgattgc cgaatattgg aatgaccgca
    15301 gccgcgccta tcaccacacg gcgcccatct ctatgctgta tggattgcac gaagccctgc
    15361 ggctgatcca tgaggaagga ctgtcaccgc ggttcatccg ccatcaactc aacagcgacg
    15421 ccttactcgc cggccttgag cctctgggcc tacggccgct ccctccggcg gcgcatcgac
    15481 tccccatgct cagctgtgtc accctgcccg agggcatcga cgatacgctg gtgcgaacgc
    15541 agctgttgca ggaccacggg attgaaatcg ggggtggcct ggggccgctg cgcgggcggg
    15601 tctggcgaat cgggctgatg ggcgagtcct gtcagcaggc gcacgtgctg acgctgctca
    15661 acgccttgga agacattttc gcgcaacacg gctggctgga tcagccaggt cgcgcggtgc
    15721 aggcggcggt cgaaacctat acccaatcac agttgtcggc gaggaggggt gcatgagtcg
    15781 aaacgggttc tggatcgtca tggcgggggt agccggcttc gtggcggcga tcacctactg
    15841 ggtcatcgcc ctcgccgtgg cagagtcacg caaagccgac atggtcgcgc ccgaacgggt
    15901 gaccagtttc attcatgcgg tgatcgatgc caatcgcgcc aactacacgc agaacgtcgt
    15961 cgacaagttg cacacccaag gcgtggtcga agccctcgaa cattggaaag aagaaaaagg
    16021 acttcccctg cccgcccaat tccttctcga atcaggccgg ctggttgccc agaaagacat
    16081 gaaactgagt ttccggctcg ccagcctcac cccgatttac gtgtggaacg gcccgaacag
    16141 tgagttcgaa cgacgcggcc tggaagtcgt catgaaggcg ccggagaaac cgttcacggg
    16201 cttttatcag cagggcgggg tgcggtactt ccaaggcatc tacgccgacc gggcggtctc
    16261 cgagagctgc gtatcctgtc acaacgggca tgcgaacagc cccaggcgcg actataaact
    16321 caacgacatc atgggcggtg tcatcgtcac cattcccatc agcgaggccc catgaccatc
    16381 gccatccgac agattcgtat cctcgacggc caaggccgca agattgagcg cggcacggtc
    16441 gtgattgaag gcgatcgcat tgtggctgcc ggaccggatc agaccgtacg tctgcctcga
    16501 ggggcacaac gcatcgacgg ccgtggcctg acgattcttc cgggtctcat cgattgccat
    16561 gtccactttt gcctgggtgc agaagccgat gtcgttgcgg ccgtcgagca ggaatcctcg
    16621 gcggtgacgt tgctgaaggc cgcggaactg gcgcggcgca ccgtgcaggc cggcttcacc
    16681 accgtgaggg atgtcggctt ccgcgaccat gccgtgtttg ccttaaagaa ggccatcgcc
    16741 tcgggactca cgccggggcc acgcatcctc gccgccggac ttgcgatctg tatgcccggc
    16801 ggacatgccc gcttcattgg ccgtgaagca caggggatcg aggccgtgcg ggccgccgtg
    16861 caggatcagc ttacggccgg cgcggaagtg atcaaggtaa tcgcctcagg cggggtccta
    16921 actccaggga cctccccgga cgaggcccag atgactgtgg aagaactgag agccgccgtt
    16981 gaagtcgccg ccgcccatgg ccggcacgtg gcggctcatg cccatggtgc ctcggggatg
    17041 aagaacgccc tgcgcgccgg ggtccattcg atcgaacatg ccacgctcat ggacgaggag
    17101 gccgccggcc tgatgactca gcgcggggtg tatatggtgc ccacactatc cgctctggcc
    17161 accacggctg cctgtccgac cggctgcggc atccccgaca gcgcccgctc aaaggccaaa
    17221 aacatggtcc ggcaacacga gaagagtttc cgcgccgccg tccggcgtgg cgtccccatt
    17281 gctctaggaa ccgatgccgg cacacctttt aattaccacg gcgacaatgc gcaggaattg
    17341 gaacgcatgg tcaccctggg catgacaccg atggatgcca tcatgacgag cacgtccgcc
    17401 gccgcgcgat tgctgcgact ttcggcagaa atcggcacca ttgaagccgg caagcaggca
    17461 gatctcttgg tggtggaagg caatccgtta agaaatatcg gactgctcct gaaacgggaa
    17521 cggatcgcag gagtgatgca aggcgggcga tttgtatcag gaccgctttc ggagacgtaa
    17581 agggaaaccg cgtccgagga agcaacgctc cggaaccgtt tcatcgtacg cgtgtggcgt
    17641 actcttcacg cgtgagcgtt accttcttat tcatctgcgt tgatacagca gctccagcgc
    17701 cgtggcgaac ccattgattc gtcccttccg gaacatctcc tccagacggc ctcgtagcgc
    17761 gcgaatcccg ccttcgtcgc ccctggcaac cacggactgc gacaccatca tctgtacggc
    17821 cacttcgagc aggcgatgct tgcgttgctc catctccggc gtcacgaatt catccagcac
    17881 ctcagccccc cgcgcctcga cgatcggttc cttgaacgta tcatacccct ggtgcgccac
    17941 cgtgcgttcc cgaatgatgg ccaattcgga tttgatccgc ggcaccattt tcatcaggac
    18001 ggcgaacact tccttgcggc cctcgtcgaa gagcttcttc tccatctcct cgagaatcag
    18061 cgcgggatcg acggtctccg gccgcatctc ctcgccccaa tgcaggcgat cgtacgtgcg
    18121 ccggctctcg ggatccccga gaacttcata ggcggagttg atctcacgga tcttctcctc
    18181 agccttgctg ttgttgggat tgcgatcggg atgatgctcg aagacgagct tccggtaggc
    18241 cttcttgatc tcgtcatcag aaatgtctcg tgacaccccg aggacgcggt aataatcgat
    18301 acgcggcata gtgcgaggga ctataccatg tccgctttgc gcgcttcacc ttgacagcgc
    18361 cgcgttctcc ccgtagcgtg gcgcgcacga cccgcacaga ggagaaccgc agcgtggctt
    18421 ggatgcatgg cgtcacgaca tatcaatggc tggtgttgtt cgtcgcctgg ctcggctggg
    18481 tattcgatgc catggatgcc acgatctatg caatcgtgct ccatcccgcg ctgcatgaac
    18541 tcttgcaagc cggcgccacc ggcggcgagg tcacgtccga acaaatcgga tggtacggcg
    18601 gcatcgtctt ttccgtgttc ctcatcgggt gggccctcgg aggtatcggc ttcggtatgg
    18661 ccgccgacta tttcggccgc accaaaacgc tgatcgcgac aattctgatc tatgccgtgt
    18721 ttacgggctt ggccgcactc gcgcaggaat ggtggcatct cgcgatctat cggtttctga
    18781 cggcgcttgg cgtcggcgga gaatgggcgg cgggagccgc catcgtggcc gagacctggc
    18841 ctgaagagaa gcgatccagg gcggcgggca tcttacagtc agcctgggcc ttcggctttt
    18901 ttatcgccgc cggctgcaac ctgctgttga aagattccgg ctggcggatg ctctttctca
    18961 tcggaatcct tccggccttt gtggccctcc tcgtccgacg atgggtgaaa gaacccgagc
    19021 gctgggtgca ggcccatgag cgagaaccgc gatcagccca cacgagctgg ctccatctcg
    19081 tcgaattgtt ccgcccctct ttacgtcgcg atacgctgat cgggtctaca ttagccttcg
    19141 tcgcggtctt cggagtctgg ggcgccacca actgggcgcc gaccctcatc cgtgggatgc
    19201 ccgatctcca gggccaggac tccgccgcgc tcgccgagaa agtgagttac gcgatcatgg
    19261 ccctgaacgc aggcgcgctt ttcggctatc tgggattcgg cccgctcgcg gaacggttcg
    19321 gacgtcgccc cgtgttcgga ttgatgtgcc ttggcagtct gatcctgctg ccggcgacct
    19381 atttcgtgcc gcacagctac gcagaagtgc tgctcctgtt gccggtactg ggatttttca
    19441 ataacggcat ctttagcggc tttccgattt atctgcccga gctgtatccg acgcggctcc
    19501 gtgccaccgg ctccggcttt tgcttcaatg ccggacgcat tctcgcctcc gcggccccct
    19561 tcgtcacggg ctggctcgtc accatgctgg gatcgttcaa caatgccgca agtaccatcg
    19621 cgttgatcta cctgctcggc ttggccatac ttccattcgc gaccgaaacc aaggggagac
    19681 cgttaccgga atgaacttcc ctcgtgagtg tgtgagcatc tcgcgccgca ggcgatgact
    19741 atgattcctc ggatgatcca gctcagcgag tccgcggtta cggttgactt cggagacggg
    19801 attgacctcc atacgaatga actggtgttg gcctttaccg ctgcggtgga ccagtcgaaa
    19861 attccgggct tcagagaagt ggttcccacc taccggtcag ccacagtcta tttcgatccg
    19921 ctactcactg atgccgacac acttatgaaa aaggtgcgtg cattgacggt ggacacagta
    19981 gtcgcgcctt cgcgcgcgtc cattactcac accattcctg tttggtatgg aggcgcagcc
    20041 ggaccggatc tgcccgatgt agcgcggcaa gccggtctca ctccagaaga agccggcgag
    20101 ctccatgcgt ccgtggcgta ccgtgtgtgt atgctgggat tcagtcccgg ctttccctac
    20161 ctgagcactg tgcccgcgcc gcttgccgcg ccgcgccttc cgacgccgcg caaatatgtg
    20221 gccgcaggct cagtgggcat cgcgggcaca cagaccggcg tgtatcccca agccagtccc
    20281 ggtgggtggc gcatcatcgg gcgcacgccg gtcgtgctat tcgatctcca caggcccaag
    20341 ccgtttctgc tcgctccggg cgaccaggtg cgattcgtgg cgatcgagga ggaagaattc
    20401 acgcaactgg caggagacca ggcatgaccc ggttgcgcca catcgatctg aactgtgacg
    20461 tcggcgaagc ggcaacgcct gagcaacggg atgtggaggc tcgtctcatg ccgtacgtga
    20521 cctcggtgaa catcgcgtgc ggcgtgcatg caggcgatgc ggcactgatg cgcagtacca
    20581 ttcaactggc gagacagtac gacctggcga tcggtgcgca tcccgggtta cctgatcggg
    20641 aatcacgcgg acgccgggag ctgccgctct cccaagggtt cgtgctcgac ctcattctct
    20701 cgcaggtgag tgatttgatg acgatcggtc aggcggaagg catccggctt tctcacgtga
    20761 aaccgcacgg agccctctac aacatggcgg cacgtgatcc ggaacttgcg gatgcgatcg
    20821 cagccgcaat cgtccaactc aatcgccggt tgattctgat cggatcggcc ggttccgaac
    20881 tcctcacctc tggaagggcg catgggctag cagtcgccgc ggaaggattt gtcgatcgag
    20941 cctaccaagc cgatggtcgt ttagtaccca ggacggaggc gcatgcggtg ctccatgatg
    21001 aatcgactgt gctcgcgcgg gcgcacgcac tggtacacga tgggactgtt gttgcagcca
    21061 atgggaccat gctccacctc cacatcgata ccctctgcct ccacggcgat accccggatg
    21121 ctcttcggct ggcccatgcg ctgcgaacaa tgttcgcgga gagcggaatt tccgtcagac
    21181 gactcgatca tgccgactaa acagccgctc attcatgtgc tgcatcccgg cctcttcacc
    21241 accgtgcagg atctcggtcg ttccggatat caacgattcg gcgtgtcggt gagtggagcg
    21301 atggacccgt gggcgctcat ggtcggcaat cgcctgctcg gtaatcctga ccaggccgcc
    21361 ggtttggaga tcacactcca ggggcctcaa ctgctgtttg agcaagcggt caggatcgcc
    21421 atcaccggtg ccgatctttc acccaccagc aacggtctcg cgcttcccat gtggaccgtg
    21481 gtcgccatgc cggctggaag ccggttacag ttcgggatgc gacgacaggg tgcccgcgcc
    21541 tatctcacag tggctggcgg catcaccggc ccaatgatac tgggaagtct ttcaacccat
    21601 gtgcggagtg gattgggcgg gctggcaggc cgagcggtga agaaatggga tcaactcttc
    21661 gtgggaactg cttcacgagc tgcagcacag cccataggtc gttcgctgcc ggaacgatat
    21721 cgaccgcggt acacgggctc tcccgccgtg cgagtcatgc cgggcccgca atcagattat
    21781 tttaccgacg acgccattcg actgttaacc agcagtccat accgggtgac gacggaatcc
    21841 gaccggatgg gctatcgctt ggagggcgcg gagctcccgc atcgtgcttc agcggagatt
    21901 gtctccgaag cggtatgcgt cggcagtctt caggttccgt ccaatcgaca accgattctc
    21961 ctgatggcag attgtcagcc gacgggcggg tatgccaagc tcgccacgat gatcggtgcc
    22021 gatcggtcgc ttgctgcgca actggcaccg ggcgacaccc tgtcctttag gatgatcagc
    22081 gcagagaagg catctgaact gtttcggtca acgtatgcgg aacttgatcg aatccttccc
    22141 cctgccgtgc ttggggcaaa accgtgaata gtgaagtctc tgcgttgatc actgccggag
    22201 atttctgcac agcccccaac gaacgagagg ggttcttcga ggcgcagagt ccttgacatt
    22261 gccctgaagc gatctattat tattcgtatg gacaccacac tgactcctcg acaaattacc
    22321 cacgatgaga aaaaagcggc tgaggccgct ttcgctggtc gcccattcaa tcccaaatgg
    22381 tctgacgcgg ctcgcatcgt ctacgacggc cttctcgccg cgatggggaa agaggctcgt
    22441 gccgtcgata cacacgacga actcgagccg gttgagccgc tggcttaatc ccctcgctgc
    22501 agttcggtcg cctaggggtg cgcataggcc tcccctctct caccttgtgg ccatcgccga
    22561 tgcgtggcat cgccacctcg cgctgcagcc taggaggctg aacgccgtcg cgcgataaga
    22621 tccttgacca gtgcccgttc ctcctcttca gtgtgtacct ctccatttaa acgcgcctcg
    22681 gtcaaccgct ccaatatggt ccggtagaca gggcctggct tcagaccaag ggcctggagc
    22741 gcctttccgg tgagagccgg ttttatgtca cgatacgtag tgagatactg ggacagagac
    22801 cgtttagcgg tgtccgagcc attcttcgcc agaaggaaca ccaggcactc ttctgagaag
    22861 ccggctaagg cggcgaccgt ttccgatggc cggagaggtg cacgcctggc taacgtccgg
    22921 cataccggct gcgtcaggaa ccgagcctgg tttatggcgg tccgttccgc ttcggtaaag
    22981 ggaaaacgtt cgagcatgtc gcggacggct tcatccggca tggtctccgc gagcgccatg
    23041 gcgtagacca cccatccatc gatggtccgg tcgagacacg acagccgata ccaatccaac
    23101 gccgcttcca gctcgctgag ccgtcgatcc agcctggcag accaggtcaa cgttgggtga
    23161 ataaaccgca acagatcaag gtcggctagc cggcgcaccg cctgcctcgg ggtgcgttca
    23221 gaaaacaaca accgcagttc atccaagagg cgctggccgg acagtcgatg gaacagttcc
    23281 atcttcaccg cgcccttaat caaggccaac gtgtctttac tcaaatgaaa gtcgaagcgc
    23341 agttcgaacc ggatcgcccg aaacacccgc gtcggatctt caacaaaact caaactatga
    23401 agcacacgaa tcagccgctc tttcagatcg cgttgcccgc cgtaaaaatc gatgagctga
    23461 ccgaaatttc gaggcctc
//
LOCUS       DAOQEW010000041        23400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_530_length_23400_cov_8.661598, whole genome shotgun sequence.
ACCESSION   DAOQEW010000041 DAOQEW010000000
VERSION     DAOQEW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23400
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_530_length_23400_cov_8.661598"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLS35_06595"
     CDS             <1..228
                     /locus_tag="PLS35_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18360.1"
                     /translation="LIPYVTSGIGVNINTKDQADSFAWRFGAGLDYPMTSLFQGAPKG
                     LMLNMETAWKRNSADGDGSSMGLLFGVRHTF"
     gene            538..1224
                     /locus_tag="PLS35_06600"
     CDS             538..1224
                     /locus_tag="PLS35_06600"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNI18361.1"
                     /translation="MSKKEIDAARRLMSLMFKAHPWHGVSMGDQAPDLVTAYIEIVPS
                     DTVKYEVDKATGFLKVDRPQRFSNFCPVYYGLIPQTYCGEQVAKLFGARAGRPDMIGD
                     GDPLDICVLTEKAIPHSDILLTARPIGGFSMADGGEADDKIIAVMRDDAVYGTLTDLK
                     ECPMTLLDRLQHYFLTYKQAPGSTAHNKVEITSMYGRDEAIKVIHASHDDYKAKFPEL
                     EFMWPASMNS"
     gene            1254..2981
                     /locus_tag="PLS35_06605"
     CDS             1254..2981
                     /locus_tag="PLS35_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI18362.1"
                     /translation="MKHTTTPSPTEPAAKGFSPGFAALGLEASLLTTLEALGYEEPTP
                     IQREAIPPLLEGRDLLGQAATGTGKTAAFALPLLQRIAHGPRQRPTALVLVPTRELAV
                     QVSEAVQRYGKELRISVLALYGGQAMGPQLQALRRGVEVIVATPGRALDHLRRKTLKL
                     ADLQVVVLDEADEMLDMGFADDLDAILEETPATKQTALFSATMPPRIASIARRHLKNP
                     IEVTIAREPVKAGAAPRVQQTAYVVARQHKVSALARVLDIAMPKSALVFCRTRLEVDE
                     VTAALNSRGYRSEAIHGGMSQVQRDRVMQAFRTGQTELLVATDVAARGLDIPSVSHVI
                     NYDLPSSLEVYVHRIGRTGRAGREGAAMTIVEPREQRLLRAVEQHTKARITIAPVPSL
                     GDLLAKRLERTKSSIQETLEAGGLEDFRRVVDALSASADPADIAAAAIKLVYRSHGGD
                     REEEEIPAVQMRAPEPYRSSRPAYGSAPQGDRGRAPRGGPSRGGRAAGTVRVYVGAGR
                     AAGIRPGDLVGAIANEAGVPSRMIGAIEVEERFSLVDVQEEAARQIIEALGRTRIKGQ
                     KVAVRLFRD"
     gene            3172..4584
                     /locus_tag="PLS35_06610"
     CDS             3172..4584
                     /locus_tag="PLS35_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI18363.1"
                     /translation="MDRLPWSRWHWLVVTALGVTWILDGLEVSIVAALGPVLTHATTL
                     HLTASEVGLTASAYLAGSVMGAIVFSYLTDRQGRKKWFMFTLMLYLAATVMTAFSWNL
                     WSFMFFRFLTGAGIGGEYAAINSAIDELIPARSRGHTDLAINGSWWLGSAAGALLTIV
                     LLNTLLIPEYLGWRLCFALGAVLGVSILLVRRVVPESPRWLMTHGSVQEAEQIVSGIE
                     AQIMRDEQLRALPAAEGSITIHGRTHATLATVARELFTAYPRRTSLGIVLMVTQSFMY
                     NAISFTYPFVLTKFYAVPSHSIGLYIVPFAIGNFLGPLLLGRFFDTIGRKQMISLTYA
                     VAGCLLASTGYLFFQGAFTSVTQTAAWSAIFFFASAGASAAYLTVSEIFPMEIRAMAI
                     AFFFVVAQGAGIAAPWLYGKLIETSAESVMYGYLLGAGMMVIGAIVELWLGVKAEGRS
                     LEHIAMPLSAQPVSSHAPHV"
     gene            4716..6272
                     /locus_tag="PLS35_06615"
     CDS             4716..6272
                     /locus_tag="PLS35_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNI18364.1"
                     /translation="MNVRPWTAALRDWQARAVADVLAQKREDYLVTATPAAGKTRFAL
                     RIAHHYLAERAASRVLVVCPTNHLRTQWASAAGQAGIQLDPALSNEQACEARDYHGAV
                     VTYQQVCLAPDVFRRACRNRKTLVILDELHHAGDGKDWGKALRESFGEAVFRLALSGT
                     PFRSDNNPIPYVRYEQGESQADFTYGYSHSIRDGVCRPILFPSYEGELTWLSDGREHR
                     ATFEDGLTFERQRERLKTALLQETWLGPVLSDAHAELQRLRKQEQADAGGLIVTMNQD
                     HARQVADLVHRLTGTRALVAVSDDPSASKTIETFSHHKTQQWLVAVNMVSEGVDIPRL
                     RVGVYATNVLTEMYFRQVVGRFVRMQDKVPKPQRAWLYLPKDATLVHYAKRIKVERDH
                     VLEEIMPAVQRTLFGTAATSLKEYIPLNGVARLDALIGADEGEPSPAGKGHPEPAVAL
                     HDQKNQLRDKHRLLVGAVARKCGMDHRQLNAELIKRTGGRVDQATIPQLERRIALLEK
                     WRDSGFDKKR"
     gene            6355..6924
                     /locus_tag="PLS35_06620"
     CDS             6355..6924
                     /locus_tag="PLS35_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18365.1"
                     /translation="MTGKKGKRPVSAAAKISRKKVGMKVVKKAAPRKQAPARRAKVTR
                     TNKSSVAVVVLSGSTPLRRNKAAELVAEELQLDLSKVNLSSVVSQRVGETEKNINRVF
                     DDAERSGSILFFDGADALFGKHRPAKDGASHYANAGAAHLLQRIEDHNGLVILTTNGK
                     SRIDQALSKQARVSVMVGIEKKKRKQKTA"
     gene            complement(7095..7265)
                     /locus_tag="PLS35_06625"
     CDS             complement(7095..7265)
                     /locus_tag="PLS35_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18366.1"
                     /translation="MPKARSAKNCYYCEAEDRIKMTFMLCGLCHRHFCSAHGVPDLEQ
                     CTKCLEASEETE"
     gene            complement(7597..9225)
                     /locus_tag="PLS35_06630"
     CDS             complement(7597..9225)
                     /locus_tag="PLS35_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNI18367.1"
                     /translation="MSLPVEKSASDELRREVLRRRTFAIISHPDAGKTTLTEKLLLYA
                     GAVHLAGAVQARSTQRQAKSDWMELEQARGISITSTMLQFDYQGARVNLLDTPGHQDF
                     SEDTYRTLMAVDSAVMVLDGAKGIEPQTKKLFAVCRKRGIPILTFINKMDQPGRHPFD
                     LLDEIERTLGMTAVPFNWPIGEGSGFQGVYDFQNRQVLFFQRTSHNQRRAPMTVEAFP
                     NPKLAETLGDAYGPLQEEIGLLTGAGTSFDRDRFLAGELTPVFFGSALTNFGVGPFLD
                     AFTKLAPPPGPRHSAQGLVQPTDETFSGFVFKIQANMDPQHRDRMAFLRICSGRFEKD
                     MMVHHARLGRKIRMTRPHRLFGRDRETIDEAYPGDVVGLVNPGLFTIGDTLTSDPSLT
                     FDPIPHFPPECFGLLRTQDISKHKQFQKGLKQLEEEGVMQIFYSPDHVRREPVLAAVG
                     ELQFDVVMSRLENEYGVKTTVERMPHSLARWIVGDAATISAVYWPSDTVRLVDQQGRA
                     VMLFTTEHLLAYCIKSNPSIKFLLREEVEQATAQ"
     gene            9537..10286
                     /locus_tag="PLS35_06635"
     CDS             9537..10286
                     /locus_tag="PLS35_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNI18368.1"
                     /translation="MNVDDIISYGELVSAEKLMLQKGMNFGVGKDYSILLMSQRANAP
                     YRDVVDDATGILIYEGHDQPRTKDCPNPKTVDQPMTTPKGTLTENGRFFRAATDFKHG
                     LREKAELVKVYEKIAVGVWCYKGFFELFDAHLISRLEKRRVFQFFLKPVQKKAFGRIG
                     ELSHKRLIPTHVKVEVWKRDQGKCVQCGLQENLHYDHDIPYSKGGSSLSAQNVRILCA
                     KCNLQKSNRIMGVFPWLWVASSRLLELGWHP"
     gene            10355..10570
                     /locus_tag="PLS35_06640"
     CDS             10355..10570
                     /locus_tag="PLS35_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNI18369.1"
                     /translation="MLNHPRDPLHGITLEKIVTTLVERHGWAELGRRLPVRCFLNNPS
                     VKSSLTFLRKTQWARERVEGMYIAELE"
     gene            10761..13079
                     /locus_tag="PLS35_06645"
     CDS             10761..13079
                     /locus_tag="PLS35_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNI18370.1"
                     /translation="MPIPLTLAFCFLLSLMQISAVSAQSTSSSTDKAVRATLETVGVK
                     LDQPAANGPAPDAAAADLQHKRAAAEKELSELTTPQAQRKGTAGNQVDGRTAERRSLL
                     QQLVQIYEQHLDALRNLEQMRQRVREAERQEKEWDGFSTPAPYSVLKIDELRDAVRSM
                     TLASQGAQTRLNMTESLTESTQRSLKASQEKARQLSERLEEVQDPAKREALTGDRDLA
                     RLRERVEGARAGMLEAEKKQIQEEVAEARHRMALLTRQRTVAEQDSAFTQQDYDKIKK
                     RLDAEHQSLTDEIERAVLEQSTQRQAVAAGEAALAAANAKDSSSKRNASRPKGDRLTA
                     LTESVELKRLQFDNANLHVELLREMLTGLEQERHIWDIRFATAQETLSVVEEREANAK
                     IAAAAKQIRGWKEYGSQQLSMVGNHISDVEDRLAEAPSAARAQVFTDKLRLYRHREDL
                     YRRALQRTDSLLGLIDNKQAEFAHREQARSVFARMKEWGRASVVMLGNAWHVELFAAE
                     DTIEVDGKNITGRRSVTVGKVVTALAILIVGYWVAGIMARFAERQAIARLQIDPNVAN
                     IIRQWALACFFLLLVIVTLMSVKIPITAFAFLGGALAIGVGFGTQNLLKNVISGLLLL
                     MERPLRVGDVIEVDGIRGMVTTIGLRSSTIRDINGVETLIPNSNLLEKNLTNWTYSSF
                     RKRYSLRIAVATGSDARHVKEKLRELAGEHGQILKEPEPYVLFEDFSEHALVFVLHYW
                     IEISPGIDAATVASDLRFMIERTFAEEKIARK"
     gene            13127..13678
                     /locus_tag="PLS35_06650"
     CDS             13127..13678
                     /locus_tag="PLS35_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNI18371.1"
                     /translation="MAPNATICKAVLHIADVDRQYYEDHSITLARHPSETDERMMVRL
                     LAFARHAHEALSFGRGLSTEDEPALWQKDATGLIELWIEVGVPDEKTIRRACGRARQV
                     WVYTYGGRAADQWWKQNGAALDRLNNLAIVNLSLEGSRALTSLAQRSMDLHCTIQDGQ
                     VLIGDGTNAAQMDLLLWKEPARE"
     gene            13700..14092
                     /locus_tag="PLS35_06655"
     CDS             13700..14092
                     /locus_tag="PLS35_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNI18372.1"
                     /translation="MKEKTRIPTDGEPVTWKSPFAALKDVVLPPAPPREQAKQLGTPA
                     PAPAKNRGRVDIIRQTAHRGGKTVTVVTGFVGISQTEKEQLAKEMQKACGVGGTVKEG
                     RIEIQGDRRDDIARILTKAGFRPVFAGG"
     gene            14099..15214
                     /locus_tag="PLS35_06660"
     CDS             14099..15214
                     /locus_tag="PLS35_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNI18373.1"
                     /translation="MAEPLKNSFGADVPRTIARMIAAVSPRFDEKAFVRSVLDGYDEL
                     ELMPRAWKIAHVLRRHLPDDYERALEMLLASLDQQSKRKTASGMGAFLFLPHVFFVAE
                     YGLDHFEASMRAQYVLTQRFTAEFSIRRYLERHQAATLARLAEWTADPSEDVRRLVSE
                     GTRPRLPWAPRLRAFQADPRPVLALLERLKDDPSLYVRRSVANNLNDIGKDHPEVLVE
                     TARRWLENATAERRWIIRHALRSAVKRGEAGALHVLGFGHTPLVAVETIRIAPSRPRI
                     GSSVGVTWEVANQASGGQRVLLDLRVHYVKANGKHSPKVFKFKTVELAARETLRFSKS
                     LALTQLTTRTHYPGLHRIELLVNGHAYPLGVFHITAG"
     gene            complement(15231..15839)
                     /locus_tag="PLS35_06665"
     CDS             complement(15231..15839)
                     /locus_tag="PLS35_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HNI18374.1"
                     /translation="MRVIVIGGTGTIGSAVVKRLSTRHEVVVVGHKKGAFQVDLASPD
                     SITSLFKAIGTCDAVVSTAGIAKFASLDDLTYDDYFIGLKNKLMGQANLVRIGRPFVT
                     NHGSFTLTSGVLSQNPMKGSASISMVNAGLEGFVRAAAIDLPRGLRVNVVSPPWVTET
                     LIARSMDPSIGIPADQVAQAYVASVEGSMTGQTLDPRQIAAT"
     gene            complement(15909..16223)
                     /locus_tag="PLS35_06670"
     CDS             complement(15909..16223)
                     /locus_tag="PLS35_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18375.1"
                     /translation="MEAAQANQVVELRLTYRYIKDHPWTVQAINGFLSAYFMEKPGFS
                     VQRHYDDLESGMHVWICDIPANMKITVLLRRLQADIPPCRHLQPAVEPSARPQYVIDS
                     PE"
     gene            16371..17207
                     /locus_tag="PLS35_06675"
     CDS             16371..17207
                     /locus_tag="PLS35_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI18376.1"
                     /translation="MLTAYNQVPVPSFMYGTAWKKDATTQLVQQAVAAGFTAIDTANQ
                     LIHYQEALVGEALLALAKQGTPRERLFVQTKFTPVNGQDHRTPYDAQADLTTQVKQSF
                     GSSLAHLHTDYLDSYVLHGPYARRGLSDADWEVWGAIESLYAAGTTRMIGISNVSADQ
                     LRLLCERATHKPMVVQNRCYAALGWDRDVREICRAHGIIYQGFSLLTANRDIFSDPDV
                     RTMAAKYGMGLAQLVFRFAMQMGMLPLTGTTDQQHMAEDLRSDQFTISTEDLQRLETV
                     GM"
     gene            17240..18856
                     /locus_tag="PLS35_06680"
     CDS             17240..18856
                     /locus_tag="PLS35_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HNI18377.1"
                     /translation="MDSLHQLEIIILLLAVVLALTTIAQKISIPYPIFLVLGGLVLGV
                     VPGLPTVTLHPDLVFLVFLPPILWAAAYFTSLREFRQNLRPISLLAVGLVLATTAGVA
                     VVAHALLPGIGWAEAIALGAIVSPPDAVSATAIGKRLRIPRRVVTILEGESLVNDATA
                     LVLYRAAVGAVVSGSFLLGGTLLNFLFAALAALAIGLAAAWLTRWALCVTEDGYTQIG
                     VTLLVPYIAWVIAESVHASAVLACVAGGITLRQYFSGAVAPATRLQARAVWELLIFVL
                     NGVIFILIGLQLGALRDAVPAGQYGPVVFAGLMVSATAIVVRFLWVPLAALIPRCLSA
                     TLRARDPMPSWKGLFLISWTGMRGIVTLAAALALPMTTGTGAPFPFRSEIILISFAVI
                     LVTLVLQGLSLPPIIRWLRLEDEDDLDREQRLAREHAATAALARLDEVMTEDWVPREQ
                     IERVRLRYLRRLEHLNRTGLNEEERPEGSVETVQRLHHEVLTAERVALIGLRDDGTIS
                     DEVLHHLEQELDVAALHQGVGERRAGKGRI"
     gene            18858..19328
                     /locus_tag="PLS35_06685"
     CDS             18858..19328
                     /locus_tag="PLS35_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI18378.1"
                     /translation="MPIHIAAAQPVDAPIIAKLVGDLLAEIVAAIKTPAFGFSLAETE
                     ARARAWLEDGSYTVLLARNGEGAAGFIALAECRALYAEGRFGIIPELYVSPERRSQGV
                     GGALVNEAKRVARSNGWARLEVTTPPLPQFDRTLAFYERQGFSVSGGRKMKVSV"
     gene            19325..19744
                     /locus_tag="PLS35_06690"
     CDS             19325..19744
                     /locus_tag="PLS35_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18379.1"
                     /translation="MIRPVVQEERTGCGIAAAAALAGVSYARAKAVAKSLGIVASDRK
                     LWSETEYVRDLVAHFGLRAARTRQPFRAWASLPDYALLAIKWRLEQGRPFWHWVVFVR
                     ENDRSYVVDSSPTLKAPLRTDFGRMRPVWFLTVTMKR"
     gene            19748..20986
                     /locus_tag="PLS35_06695"
     CDS             19748..20986
                     /locus_tag="PLS35_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNI18380.1"
                     /translation="MNIAIIGGGPAGLMAAEVALAGGAQVALYDSMPSVGRKFLLAGK
                     GGLNLTHSDPSDLFLSRYGERRAQVAPWLAEFGPDALRTWARGLGIETYVGSSGRVFP
                     TDMKAAPLLRAWLRRLRQDGLTIHVRHRWCGWDEKKTLRFETPHGATAVRADAVILAL
                     GGGSWPQLGSDGAWVPVLAGRSIAIAPLRPANCGFDVGWTEHFRTKFAGHPVKTVGVV
                     AKSPTGAVMRRMGEFVITDTGVEGGVIYAVAPFLRDEIEANGRGTLRLDLAPDRELPR
                     LIKDLSQPRGKKTMATHLQRRAHIDGVKAGLLREIVSKEDFADPTRLAAAIKALPLTL
                     TAPRPLHEAISTAGGVTCEALDKRLMLKALPGVFCAGEMVDWEAPTGGYLLTACFAGG
                     RTAGAGALAWLAERAERAKA"
     gene            20973..21530
                     /locus_tag="PLS35_06700"
     CDS             20973..21530
                     /locus_tag="PLS35_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI18381.1"
                     /translation="MPRHNVTIDGMALVVPGIGNSGPRHWQTLWEQQHPRWQRVQQRD
                     WDRPVLTEWLPALESALAGISAPAVLIAHSMGCLLVAHWAQQASVPVRAALLVAPPDP
                     RSPAFPASARGFETVPSGRLPFPTLLVASSNDPFGSVAYAKQCATVWGSAFAEAGAIG
                     HINAESEIGAWPAGLVLLERLLASS"
     gene            complement(21594..22376)
                     /locus_tag="PLS35_06705"
     CDS             complement(21594..22376)
                     /locus_tag="PLS35_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNI18382.1"
                     /translation="MAGSLLRIACLHLAPIPGAIAQNRHLLTQAITRAACSGASWIIT
                     PELAVSGYTFADTLGTEWIEPQPDHWMSRICRLAARLRVTLFLSHPEKEPRSGQLYNT
                     VFAIGPDGRIAGSHRKINALRVGSEAWSTPGTDATAFRLAPFGRVGLLICADAYTPGI
                     AKSLKAQGATVLVSSAAWAPGLHGPNDEWERCTKDTGLPLFVCNRTGHDRTLDFRKAE
                     SVVAHNGKRLLSLSCEQSAIFLFDWNLTTGTLADRGHQRILI"
     gene            22520..>23400
                     /locus_tag="PLS35_06710"
     CDS             22520..>23400
                     /locus_tag="PLS35_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNI18383.1"
                     /translation="MNHPIVPPSRKRLGQWLLLLVLLLALLCVATILALPWLLNRPAS
                     MAALLQQVEARTGHRISFEQSHVEIFPSPRVTLMQPRLYAATSPDPLLFAERVEVALQ
                     WLPLLEGSLVVKDLVIDRPRVTLRRSPNGNWALGNEHSPGPDSEPTPPFALLQVVRNL
                     LVVQGMVTLVDESASPSTSLNIIVMQAALSSEMLGRRAKLHLSGELPQAGGRAAFLWE
                     GSITQQGDEDGIQAEGDLRLHQLNVRQVLSAWMGDGRITDGFSQPAQLTAHVRWKPGA
                     AGYDLLADDVRAELADVS"
BASE COUNT         4581 a         6522 c         7508 g         4789 t
ORIGIN      
        1 ctgattccgt atgtcaccag cggtatcggc gtgaacatca acaccaaaga tcaggcggat
       61 tcgtttgcct ggagatttgg cgcaggcttg gactatccca tgacgagcct ctttcagggc
      121 gcgcccaaag gcctgatgct gaatatggaa acggcctgga agcgtaattc cgccgacggg
      181 gatgggtcct cgatgggcct gctcttcggt gtccgccata cgttttaatt ctcaggtact
      241 cctctcaacc acgtgccggg cggtcagtga tggtcctttc gatcctgacg gcccggcccc
      301 ttcggctgtt ttcggcacat taccgcgaga attcatgacg gccgcgggtc cgcgccgaga
      361 cgcgactttt taccctcctg tttccttcgc ccccaatgtg ttataagctg actgtttctc
      421 tagcctgata ctcctgctct attgcgggtg tgtcgtcgaa ttaatccaga ccttgccgga
      481 tcgtacgatc gtgagaccgt caattccgaa ggtgttcaca agggggactg aagcaggatg
      541 agcaagaagg aaatcgatgc ggcgcggcga ctgatgagcc tgatgttcaa agcgcatccc
      601 tggcatgggg tgtccatggg ggaccaggcg ccggatctcg tgacggccta tatcgaaatc
      661 gtaccgagcg atacggtgaa gtatgaagtg gataaggcca cgggctttct caaagtcgac
      721 cgtccccagc ggttctcaaa cttctgcccc gtgtattacg gactgattcc gcaaacctat
      781 tgcggtgagc aagtggcgaa gctgttcggt gcccgcgccg gccggccgga catgatcggc
      841 gatggcgatc ctctggacat ctgcgtactc accgagaagg cgattcccca cagtgatatt
      901 ttattgaccg cccggccgat cggcggattc agcatggcgg acggcggcga agcagacgac
      961 aagatcatcg cggtgatgcg ggacgatgcc gtttatggaa cgttgaccga tctcaaagaa
     1021 tgtcccatga ccctccttga ccggttgcag cattatttcc tgacctataa gcaggcaccg
     1081 ggctccacgg cacacaacaa ggtggagatt acgagcatgt atggccgcga cgaagccatc
     1141 aaagtcatcc acgccagcca cgacgattac aaagccaagt ttcccgagtt ggaatttatg
     1201 tggcccgcca gcatgaatag ctagtcttac gaagaaaggt ttagcccctc ctgatgaaac
     1261 acaccacgac gccatcgccg acagagccgg ctgccaaggg attttcccct ggatttgccg
     1321 cgctcggatt agaagcctcg ctcctgacta cccttgaagc gttgggctac gaagagccca
     1381 ccccgattca acgggaagcg attcccccgt tgctggaagg gcgtgatctg cttggacagg
     1441 cagccaccgg aaccggaaaa acggcggcct ttgccttgcc gctcctgcag cggattgcgc
     1501 atggtccacg gcagcggccg acggcgctgg tgttggtgcc gacccgagaa ctggccgtgc
     1561 aggtcagcga agcggtgcag cggtacggca aagagctgcg gatctccgtg ctggccttgt
     1621 acggtggcca ggcgatgggc ccgcaactgc aagcactcag gcgtggagtg gaagtcattg
     1681 tggcgacgcc cggccgggca ttggaccatc tccgtcgcaa gaccttgaag ctggctgatc
     1741 tgcaggtcgt ggtgttggat gaggcggacg agatgctcga catgggtttc gccgacgacc
     1801 tcgacgccat tctcgaagaa acgccggcga cgaaacagac cgcgttgttt tcggcgacca
     1861 tgccgccccg gattgcgtcc atcgcccgcc ggcatctgaa gaatccgatc gaagtcacga
     1921 tcgcgcggga gccggtgaaa gccggcgctg ctccgcgtgt gcaacagacg gcgtatgtgg
     1981 tggcccgcca gcacaaggtg tcggcgctgg cccgtgtctt ggacattgcc atgcccaagt
     2041 cggctctggt gttttgtcgc accaggctgg aggtggatga agtgacggcg gcgttgaata
     2101 gccgggggta ccgatctgaa gcgattcatg gcgggatgag ccaggttcag cgggatcgtg
     2161 tcatgcaggc ctttcgcaca gggcaaaccg aactgctggt ggcgaccgac gtggcggctc
     2221 gcggattgga tattccgtcc gtctcgcacg tgatcaatta cgacttgcct tcatcgctgg
     2281 aagtgtacgt gcatcggatc ggccgcacag gccgggccgg ccgggaaggt gcggccatga
     2341 cgattgtcga gccgcgcgag cagcggctct tgcgcgccgt cgagcagcac accaaggcgc
     2401 gcattacgat cgcgccagtg ccgtcgctcg gagatctgct ggccaagcgg ttggagcgaa
     2461 cgaagtcgtc gattcaagaa accctggaag cgggcggcct cgaagatttt cggcgtgtgg
     2521 tggacgcctt gtccgcatcg gccgacccgg cggatattgc ggcggccgcc atcaagttgg
     2581 tctatcgatc acacgggggc gaccgggagg aagaagaaat tcccgccgtc cagatgcgcg
     2641 cgccggagcc ctatcggtca tcacgtccag cgtatggctc ggctccgcaa ggggatcgcg
     2701 ggcgagcgcc ccggggggga ccgagtcgtg gtggtcgagc ggccggcacc gtgcgcgtgt
     2761 acgtcggcgc ggggcgggcg gcaggcatca gaccgggcga tctggtcggc gcgattgcca
     2821 atgaagccgg tgtgccgtcg aggatgatcg gggcgatcga agtcgaagag cgattttccc
     2881 tggtggatgt gcaggaagaa gcggcccggc agattatcga ggccttgggc cggacgcgca
     2941 tcaagggaca aaaggtcgcg gtcagattgt tcagagactg aaacactccc acatgttcaa
     3001 accggccggg gtgccccaat gaaggccctg gccggcttgt gctctcgtcg ccccttcctt
     3061 ctcaaccttt cgcccgccag ggaattcaat aggcgtccgt gttacctgca catggtacga
     3121 tcacgcatgc ctgcctcaga aagctccgtg acaacgagca tcccgcaacg tatggaccgc
     3181 ctgccatggt cgcgctggca ttggctggtg gtgacggccc tgggcgtcac ctggattctg
     3241 gacggactgg aagtgtccat tgtggccgcg cttggcccgg tcctgacgca tgcgaccacg
     3301 ttgcatttga cggcttctga agtcggcctc accgcttcgg cctatttggc cggatcggtg
     3361 atgggcgcga ttgtcttctc ctacctgact gatcggcagg gacggaagaa gtggttcatg
     3421 ttcaccctca tgctctacct ggctgcgacc gtgatgacgg cgttctcctg gaatctctgg
     3481 agctttatgt tctttcgttt cttgacgggt gccgggatcg gcggggaata tgccgcgatc
     3541 aattccgcca tcgatgaact cattccggcg cgcagtcgcg gacatacgga tctggccatc
     3601 aatggcagct ggtggctggg cagcgcggcc ggtgcgctgt tgaccattgt gctcctgaac
     3661 accctgctga ttccggaata tctgggctgg cgtctctgtt tcgccttagg agccgtactg
     3721 ggggtgagca tcctgctcgt gcgacgggtc gtaccggaga gtccgcgatg gctcatgact
     3781 cacggcagcg tgcaggaagc ggagcagatc gtgtccggca ttgaagcgca gatcatgcgc
     3841 gacgaacaac tgcgggcgct tccggcggcc gaaggctcga ttaccattca cggacggacg
     3901 catgcgaccc tggctaccgt ggcccgggag ctgttcacgg cctatccccg tcgcacctcg
     3961 ttgggaattg tgctcatggt gacgcaatcg ttcatgtaca atgcgatttc attcacctat
     4021 ccgtttgtgc tgacaaaatt ttacgccgtg cccagccaca gcattggtct gtatatcgtg
     4081 ccgtttgcga tcggcaattt tctgggcccc ttgttgctgg gccgcttttt cgataccatc
     4141 ggccgcaagc aaatgatcag cctcacctat gcagttgccg gttgtttgct ggcgtccacc
     4201 ggctatctgt tttttcaggg agcctttacc tctgtcacgc aaactgccgc gtggtcggcc
     4261 attttctttt ttgcttcggc cggggcgagt gccgcctatc tgaccgtcag cgagatcttt
     4321 ccgatggaga tccgcgcgat ggccattgcc tttttcttcg tcgtagccca gggtgcgggc
     4381 atcgcggccc cgtggttgta cggcaagctg atcgaaacat cagccgagag tgtcatgtat
     4441 ggatatctgc tgggagccgg gatgatggta atcggtgcca tcgtggagtt gtggttaggg
     4501 gtgaaggccg aagggcgatc actcgaacat atcgcgatgc ccctttccgc ccaacctgtc
     4561 tcgagtcatg cgccgcacgt ctgacagcta ttcccatagg atgcacgcgt aatctgccca
     4621 cgggctgatg ccacggaatg tgtcacggcg gcggcgggac aagtgcgaat accattcatg
     4681 tgtaagtata gcgatcaagg tgaaatgagg cgcgggtgaa cgttcgtccg tggacagccg
     4741 cgttgcgcga ctggcaggcg cgagcggtgg ccgatgtgtt ggcgcaaaag cgcgaagatt
     4801 atctcgtgac ggcgacgccc gctgcgggaa aaacgcgctt tgcgttgcgg atcgcccatc
     4861 attatctggc ggagcgtgcc gccagccgtg tgctggtagt ctgtccgacg aatcatctgc
     4921 gaacacagtg ggcgtcggca gcagggcagg ccggtattca attggacccg gccttgtcca
     4981 atgaacaggc ttgcgaggcg cgtgattatc atggcgcggt agtgacctat cagcaggtct
     5041 gtctggcgcc ggacgtgttt cggcgcgcct gccgaaaccg gaagactctg gtgattctcg
     5101 acgagttgca ccatgccggg gacggcaagg attggggcaa ggcgcttcgt gaatcattcg
     5161 gcgaagccgt gtttcgcctg gccctgtcgg gtacgccctt ccggtcagat aacaatccca
     5221 ttccgtatgt gcggtatgag caaggcgaga gccaggcgga ttttacctat gggtattcgc
     5281 actccatacg ggacggcgtc tgccgtccga ttctgttccc cagttacgag ggagaactga
     5341 cctggttatc cgacgggcgg gaacaccggg ccacgttcga agacggattg acctttgagc
     5401 gccagcgcga gcggctcaaa accgccttgc tccaggaaac ctggctgggt ccggtgttga
     5461 gtgatgcgca tgccgagttg cagcgcctgc gcaaacagga acaggcggat gcgggcggtc
     5521 tgatcgtgac catgaatcag gaccatgcac ggcaagtggc cgatctggtg catcgactca
     5581 cgggcacgcg tgcgctggtc gccgtctccg acgatccttc cgcctcgaaa acgatcgaaa
     5641 ccttttccca ccacaaaacg cagcaatggc tggtggcggt gaacatggtg agcgaaggcg
     5701 tcgatattcc gcgcctccgg gtcggggtgt atgcgaccaa cgtgttgacg gaaatgtatt
     5761 tccgccaggt cgtcgggcgc tttgtgcgga tgcaggacaa agtgccgaaa ccgcaacggg
     5821 cctggctcta cctgcccaaa gacgccacgc tggtgcatta tgccaagcgg atcaaggttg
     5881 agcgcgatca tgtgctcgag gagatcatgc cggcggtgca gcggacgctg ttcgggacgg
     5941 cagcgacgtc gctgaaagaa tacattccgc tcaatggtgt ggcgcggctc gatgccttga
     6001 tcggcgcgga tgaaggcgag ccatcccctg cagggaaggg ccacccggag ccggcagtgg
     6061 cgctgcatga ccaaaagaac cagttgcggg ataaacatcg gcttctcgtg ggagccgtgg
     6121 cgagaaagtg tggaatggat catcggcaac ttaacgcgga attaatcaag cggaccggcg
     6181 gccgcgtgga tcaggcgacc attcctcaat tagagcggcg gattgcccta ctggagaagt
     6241 ggcgggattc cggattcgac aaaaagcgct gagcaatcaa agtatggtaa gcatagcctg
     6301 cgcgatgagt gagcaaagaa cggtatgaag caatcattgg agaggaagag gaccatgaca
     6361 gggaagaagg gtaagcggcc ggtgagcgcg gcagcgaaga tatcgagaaa aaaagttgga
     6421 atgaaggtcg tgaagaaggc tgcccccagg aagcaggcac cggcgcgccg cgcgaaggtg
     6481 acccggacga acaaatcctc ggtggcagtc gttgtcttgt ccggttccac gcccctgcgc
     6541 aggaacaagg cagcggaatt agtcgcggaa gaattgcagc tggacttatc gaaagtgaat
     6601 ctctcctctg tggtaagcca gcgagttggg gaaacagaga aaaatatcaa ccgggtcttc
     6661 gacgacgctg aacgcagcgg gtcgattttg ttctttgacg gagccgatgc cttgttcggc
     6721 aaacatcggc cagccaagga tggcgcaagc cattacgcca atgccggagc agcccatctg
     6781 ctgcagcgca tcgaagacca caacggcctg gtgattctga cgaccaacgg caaaagccgg
     6841 atcgaccaag ccctatcgaa gcaggctcgc gtatcggtga tggtgggcat cgaaaagaag
     6901 aagcggaaac aaaagacggc ctgatgagcc gtccacttca aagggcgaga tcgaagtcct
     6961 gaatacagga tgctgcaaaa gggttgttca acaaagccgc agcaagcgta aggctgagga
     7021 gtaactcaga agtccattga gggcccgagt gatgcgagaa cgccgctgac agactggttc
     7081 ggcatttttg cggcttactc cgtttcttcg ctcgcctcca ggcacttcgt gcattgctcg
     7141 aggtcgggca cgccatgggc gctgcagaag tgccgatggc agagaccgca gagcataaat
     7201 gtcattttaa ttcgatcttc cgcttcgcag tagtaacagt tttttgcgct tcttgccttg
     7261 ggcatggtcc ttctcctcgc atcattgtcc ggttcatagg tcgtctcgtc acttgacggt
     7321 gccactcggg gtcggcggcg tattcgaacg cacccattcc aggctcggca tgggaacggc
     7381 agcgtctcaa ttgatgacgg gaggcgggca agtcacccag gccgagttag cctgaggcgc
     7441 taaggacagc aaaaggtgtg atcgtgctcg cagtcatcct accaggaagc cccgaccggc
     7501 tttcctggac ggttagtttg ttataacaca ttttctcatg cgtctgcgca gcagcacgtt
     7561 gcgtgggagc cccgtctcct tgcgagaacg gctcgtctac tgtgcggttg cctgctcgac
     7621 ttcttcccgc aagaggaact tgatggaggg gttggatttg atgcagtagg ccagcaggtg
     7681 ttcggtggta aacaacatga cggcgcggcc ttgttgatcc acgaggcgga cggtatcgga
     7741 gggccaatag acagcagaaa tggttgccgc atcaccgacg atccagcgcg ccagactatg
     7801 cggcatacgc tcgacggtgg ttttgactcc gtattcgttc tccagtcgcg acatcaccac
     7861 atcaaactgc agttcaccca ccgcagccaa caccggttcc cggcgcacgt ggtcgggtga
     7921 ataaaaaatc tgcatgaccc cttcttcttc gagctgcttg agtcccttct gaaactgctt
     7981 gtgcttggag atatcctgcg tccgcaacag gccgaaacat tccggcggaa agtgcggaat
     8041 gggatcgaag gtcagtgaag gatcggaggt cagcgtatcg ccgatggtaa acagtcctgg
     8101 attcaccagt cccactacat cgccggggta ggcttcatcg atcgtttcgc ggtcgcggcc
     8161 gaaaaggcga tgcgggcgcg tcatgcggat ttttcgaccc agccgggcgt gatggaccat
     8221 catgtccttt tcgaatcggc ccgagcagat gcgcaggaag gccatgcggt cgcgatgttg
     8281 cggatccatg ttcgcctgga tcttgaatac gaaaccggaa aaggtctcgt ccgtcggctg
     8341 caccaggccc tgggcgctgt gtcgcggtcc gggaggcggg gcaagtttcg tgaaggcgtc
     8401 gagaaacggt ccgacgccga agttggtgag cgcgctgccg aagaacaccg gagtcagttc
     8461 gccggccagg aagcggtctc gatcgaacga ggtgccggcg cccgtcaaga gcccgatttc
     8521 ctcctgcagc gggccatagg cgtcgcccag cgtctccgcg agtttcggat tcgggaaggc
     8581 ctcgaccgtc atcggggcgc gacgttggtt gtgcgaggtg cgctggaaga acaggacctg
     8641 gcggttctga aaatcgtaca cgccttgaaa accggatcct tcgccgatgg gccaattgaa
     8701 cgggaccgcc gtcatgccca gcgtgcgctc gatctcatcg agcagatcga aggggtgccg
     8761 gcccggctgg tccatcttgt tgatgaaggt caggatgggg atcccgcgtt tgcgacacac
     8821 ggcaaacagc ttcttggttt gcggctcgat acccttggcg ccgtccagca ccatgaccgc
     8881 gctatccacg gccatgaggg tacgataggt gtcttcgctg aagtcctggt ggcccggcgt
     8941 gtcgaggaga ttgacccgcg ccccttgata atcgaattgc agcatggtcg aggtaatcga
     9001 aatgccgcgt gcctgttcca attccatcca gtcggatttg gcctggcgtt gggtggagcg
     9061 agcctgcacc gcaccggcca agtgaaccgc gcccgcatag agcagtaatt tttccgtcaa
     9121 cgtggttttc ccggcatccg ggtgggaaat aatggcgaac gtgcgccgac gcagcacttc
     9181 tcgccgtaac tcgtcggagg cggacttctc aaccggcagg gacataacgt gctcttggtg
     9241 gttaggggaa tcgacggaac agggggtatg tggccgcctg gcgtcgctgg gttgcaacgc
     9301 cttcttttat cagtctgagc ggggcggcgg caagaagggg gagagggggg ccgggcaacc
     9361 tgtgtggtcc ccatgctcgc gcaacgcgcg gtcgcggact cccctcgttg ggtgatgtaa
     9421 gtcccttgag agattggccc tcatggagac atacacctca cttggcatat actcccactg
     9481 ccgcataacg ttctgaatgt ggagtctgaa tatgtctagc attccatgag ggccgcatga
     9541 acgttgatga cattataagc tacggtgagt tggtcagcgc tgaaaaattg atgcttcaga
     9601 agggaatgaa ttttggagtg ggaaaggact attccattct actcatgtct caacgagcca
     9661 acgctcctta tcgagatgta gttgatgatg ccacaggaat actgatttat gaaggccatg
     9721 atcagccacg cacaaaggat tgtcctaacc ccaagacggt ggatcaacca atgaccacgc
     9781 caaagggaac attgaccgaa aatgggcggt tctttcgagc cgccactgat ttcaagcatg
     9841 gactaagaga aaaggcggag ttagtcaaag tctacgagaa aatcgctgtt ggggtatggt
     9901 gctacaaggg attctttgag ctgtttgacg cgcaccttat atcacggcta gaaaaaagaa
     9961 gagtgttcca gttcttcctc aagcccgttc aaaagaaagc ctttggtcga ataggggaac
    10021 tatcacacaa acgactcatt cccacgcatg tgaaagtgga agtttggaaa cgagatcagg
    10081 gcaaatgtgt tcaatgtggt ttgcaggaga atcttcatta cgatcacgac ataccctatt
    10141 cgaagggtgg aagcagtctt tcagcgcaaa acgttcggat cctatgcgca aaatgcaacc
    10201 tgcagaaatc gaacagaatc atgggtgttt tcccctggct atgggtggca tcgagccgtc
    10261 tgctggagct tggatggcac ccatgattct gagggacgtt gccagagcaa aagttgaact
    10321 agtttagaat gcgaactaaa gcagctgcag taacatgttg aatcatcccc gcgatccttt
    10381 gcatggcatc acgctggaga agatcgtcac cacgcttgtc gagcggcatg ggtgggctga
    10441 acttggccgt cgtctgccgg tgcgctgctt cttgaataac ccgagcgtca aatcgagtct
    10501 cacgtttctc cgcaagaccc agtgggcccg agagcgggtt gagggaatgt atattgcgga
    10561 actggagtga gggcgcagcc gttcatacgg aatccgttct tcgtcggtgc cacatcagtc
    10621 gaacgacaca cgacttgtcc cacagggctt tgctaagctg gacgcgcgtc ctccctgtca
    10681 acgttcctgc gacttgatca atcccgccca cctctgtaga tatgaagaag actcgcttac
    10741 gatcgcggag gttcacgttc gtgccaatcc ccctcacgct tgccttctgc ttcctgctct
    10801 cgttgatgca gatctctgct gtctcagctc agtcgacctc ctcgtctacc gataaggcgg
    10861 ttcgtgccac gttggaaacg gtcggggtca agttggacca gccggcggcc aatggaccag
    10921 ctccagatgc cgccgccgcg gatctccaac acaaacgtgc agcggcagaa aaggaactga
    10981 gcgagctcac cacaccacaa gctcagcgca aaggaacggc ggggaaccag gtggatggcc
    11041 gcacggcgga gcggcgctcg ctgctgcagc agttggtgca aatctatgag caacatctcg
    11101 atgccttgcg caatctcgag cagatgcggc aacgggtgcg ggaagctgaa cgccaggaaa
    11161 aggagtggga tggattttct acgccggcgc cgtattcggt gttgaaaatc gatgagctgc
    11221 gcgatgcggt tcgatcgatg accctcgcct cacaaggcgc ccaaaccaga ctcaacatga
    11281 ccgaaagcct aaccgagagc acgcagcgca gcctcaaagc ctcgcaggaa aaagcccggc
    11341 aattgtcgga gcgtctcgaa gaggtccagg atcctgccaa gcgggaggcg ctcacaggag
    11401 accgcgatct ggctcgattg cgcgagcgag tggaaggcgc acgagccggc atgttggagg
    11461 cggaaaagaa gcagatccag gaagaggtgg cggaagcgcg tcatcgtatg gcattgctga
    11521 cccgccagcg caccgttgcc gaacaggatt cggcatttac ccaacaggat tacgacaaaa
    11581 tcaaaaagcg gctggatgcc gaacatcaat cgttgacgga tgaaatcgag cgagcggtcc
    11641 tcgaacaatc gacgcaacgc caggcggttg cggccggaga agctgcgctc gctgcggcga
    11701 atgccaaaga ctcttcgtcc aagaggaacg cctcccggcc gaagggggat cgcctgaccg
    11761 cgctcaccga atcggtcgaa ctgaagcgtc tgcaattcga caacgccaat ctgcacgtgg
    11821 agttgctgcg cgagatgctg acgggtcttg agcaggaacg acatatttgg gacatccgtt
    11881 tcgcgactgc ccaggaaacg ctctccgtcg tcgaagagcg ggaggcgaat gcgaagatcg
    11941 ccgccgccgc caaacagatt cgaggctgga aggaatacgg cagtcagcaa ctgagcatgg
    12001 tgggcaatca catcagtgat gtggaagacc gccttgcgga ggctccttcg gccgccaggg
    12061 cgcaagtgtt cacggacaaa ctccggctct atcgccaccg tgaagatctg taccggcgcg
    12121 cgctccagcg gacggattcc ttgttggggt tgatcgacaa caaacaggcg gaatttgccc
    12181 accgtgagca ggccaggtca gtgttcgcgc gcatgaagga gtgggggcga gcctccgtgg
    12241 tgatgctggg gaatgcctgg catgtggaat tgtttgccgc cgaagacacc attgaagtgg
    12301 acggcaagaa catcaccggt cgacggagcg tcacggtcgg gaaagtcgtg acggcgctgg
    12361 cgattttgat cgtcgggtac tgggtggcgg gaatcatggc ccggtttgcc gagcgccagg
    12421 ccatcgccag gctgcagatc gatcccaacg tcgccaacat cattcggcag tgggccctgg
    12481 cctgcttttt cctcctcctt gtgatcgtca ccctcatgtc agtgaaaatt cccatcaccg
    12541 ctttcgcgtt tctcggcggc gccctggcca tcggggtggg attcggtacg caaaatttgc
    12601 tgaagaacgt catcagcgga ctgctgctgc tcatggaacg gccgctgcgc gtcggcgatg
    12661 tcatcgaggt ggatggaatc agaggcatgg tcaccaccat cggtctgcgc tcctcgacaa
    12721 tccgggacat caacggcgtg gaaaccttga ttcccaacag caatctgctc gaaaaaaatc
    12781 tgacgaactg gacctattcc agctttcgta aacggtattc gctccgcatc gcggtggcga
    12841 ccgggtcgga tgcgcggcat gtgaaggaga agctgcgtga gctggccggt gagcacgggc
    12901 agattctcaa ggagccggag ccgtatgtat tgtttgaaga tttcagcgag catgcgttag
    12961 tgtttgtcct ccactactgg atcgagatca gtcccggtat cgacgcggcc acggttgcca
    13021 gcgatctgcg cttcatgatt gaacggactt ttgctgaaga gaagatcgcg aggaaataac
    13081 ggccgcctgc ctcatcgggc tcctccctct gttttcaagg aatcgcatgg cccccaacgc
    13141 gacgatttgt aaggcggtcc tccatattgc cgacgtggat cgccagtatt acgaagacca
    13201 cagcatcacc ttggcgcggc atccatcgga aaccgacgag cgcatgatgg ttcgcctgct
    13261 tgcgtttgcc cgtcacgcgc atgaagccct gtcgtttggc cgtggattga gcaccgagga
    13321 tgagccggcg ttgtggcaga aggatgcgac cggtctgatc gagttatgga tcgaagtggg
    13381 tgtgccggac gaaaagacga ttcgccgggc ctgcggtcgc gccaggcagg tgtgggtcta
    13441 tacctatggt gggcgtgcgg cggaccaatg gtggaagcag aacggtgccg cgctcgaccg
    13501 gttgaacaat ctcgccatcg tgaatctgtc tttggaaggg agtcgtgcgc tgacgagtct
    13561 ggcgcagcgg agtatggatc tgcattgcac cattcaagat gggcaggtgt tgattggaga
    13621 cgggacgaat gcggcgcaga tggacctgtt gctgtggaaa gagccggcgc gtgaatagtc
    13681 aacgaggaag aggttcggca tgaaagaaaa gacgcgcatt cctaccgatg gagaacccgt
    13741 gacgtggaag agcccgtttg cagcgttgaa agacgtggtc ttgcccccgg ccccgccgcg
    13801 agagcaggcc aagcagctcg ggacaccggc accggctccg gcgaagaatc gcggccgggt
    13861 ggatatcatc aggcagacag cgcatcgagg cggcaagacg gtcacggtgg tcacgggatt
    13921 tgtcggcatc agtcagacgg agaaagaaca gctggcgaag gagatgcaga aggcctgtgg
    13981 cgttggaggg acggtgaagg aaggtcgcat cgagattcaa ggagaccggc gtgacgacat
    14041 cgctcgtatt ttgaccaaag cggggttccg gccggtcttt gccggtggct gaacagtcat
    14101 ggcggaaccg ctgaagaata gtttcggggc cgatgttccc cggaccattg cgcgaatgat
    14161 tgccgccgta tcgcctcgat tcgacgagaa ggcattcgtc cggtcggtgc tggatggtta
    14221 tgacgagttg gaattgatgc cgcgcgcctg gaagatcgcg catgtcttgc gacgccattt
    14281 gccggatgac tatgaacggg ctcttgagat gcttctggcg tcgcttgatc agcagtcgaa
    14341 gcgaaagact gcctcaggca tgggcgcctt tttgtttctt ccgcatgtct tcttcgtggc
    14401 tgaatatgga ttggaccatt tcgaagcctc catgcgcgca cagtatgtac tgacgcaacg
    14461 attcacggcc gagttctcca tccgccggta tctcgagcga caccaggctg ccacgttggc
    14521 ccgtcttgcg gaatggacgg ctgatcccag tgaagacgtg cgacgcctgg tctcggaagg
    14581 cactcgcccg cgattgccct gggcaccgag actgcgcgcc ttccaggctg atccgcggcc
    14641 ggtgctggcc ctgttggagc ggttgaaaga cgatccgtca ctctacgtgc gccgcagtgt
    14701 ggctaacaat ctgaatgaca tcgggaaaga ccatcccgag gtgctggtcg agacggccag
    14761 gcggtggctg gagaacgcaa cggccgagcg gcggtggatc attcgccatg ctctccggtc
    14821 ggcggtcaag cggggagaag caggagcgct ccatgtgctg ggcttcggcc acacaccgtt
    14881 ggtcgcagtg gagacgatcc gcatcgcgcc gtcacggcct cggatcggat cgtcagtggg
    14941 cgtcacctgg gaggtggcga atcaagcctc cgggggacaa cgggtgctgc tggatttgcg
    15001 cgtccactac gtcaaggcga atggcaaaca cagtccgaag gtgttcaaat tcaaaaccgt
    15061 cgaactggcg gcgcgggaga cgcttcgctt cagcaaatcg ttggccctca cgcaactcac
    15121 cactcgcacc cactatcccg gcttacaccg cattgagctg ctcgtgaacg gccacgccta
    15181 tccgttaggg gtgtttcaca tcacggcagg gtgaccggcg cgttgtcgca ctaggttgcg
    15241 gcgatctgtc tgggatccag cgtctgtccc gtcatgctgc cttccacgct tgccacatag
    15301 gcctgcgcga cctgatcagc agggatgccg atggaaggat ccatacttct ggcgattaag
    15361 gtttccgtca cccaaggcgg gctgacgacg ttcactcgca ggccgcgcgg cagatcgatg
    15421 gcggcggcgc ggacaaatcc ttccagtccg gcattgacca tactgatgga ggcgctgccc
    15481 ttcataggat tctggctgag aactccgctg gtgagcgtga aagagccgtg gttggtcacg
    15541 aagggccggc cgatgcgtac cagattcgcc tggcccatga gtttgttctt caggccgatg
    15601 aagtagtcgt cataggtcag atcatccagg ctcgcaaact tggcaatccc ggcggtgctg
    15661 accacggcat cacaggtgcc gatcgccttg aacaggcttg tgatggaatc cggcgaggcc
    15721 aggtccacct gaaaggcgcc tttcttgtgc ccgaccacca ccacctcatg ccgcgtggag
    15781 agccgcttca cgacggccga tccgatagtg cctgttcctc cgatgacgat cactcgcatg
    15841 gtaatcctcc gtcgttcatg tttcgcgcag tgtagcgagg aggcctggag cggcgcaagc
    15901 aggcgccctt attccgggct gtcgatcaca tactgcggcc tggccgatgg ctcgacagcg
    15961 ggttgaagat gccggcaggg gggaatatcg gcctgcagcc gccgtaacaa caccgtgatc
    16021 ttcatgttgg ctggaatgtc gcagatccac acgtgcatgc ccgattcgag atcgtcataa
    16081 tgccgctgga ccgaaaatcc cggcttttcc atgaaatagg ccgagagaaa tccgttgatg
    16141 gcttgaacgg tccagggatg gtctttgatg tatcggtagg tcagtcgcag ttccacgacc
    16201 tgattcgcct gtgctgcttc catgtctgtt ccgctcctct cgtggtgacg gcgcactcta
    16261 gcaggttgtg gagaacggtc gccagcgtcg tccggtgcct tggcccaacc aggtgaaaga
    16321 ttgagaccgc cgcaaggatc tgtgtaaggt acggcaggga ggatctggtc atgttgactg
    16381 cctataatca ggtgcctgtt ccctcgttca tgtacggaac ggcgtggaaa aaagacgcaa
    16441 cgacacaatt ggtacagcaa gcggtggcgg cgggcttcac ggccatcgat acggccaacc
    16501 agctcatcca ctaccaggaa gccctggtcg gagaggcctt gttggcgttg gcaaagcagg
    16561 gcacgccgcg tgagcggctg ttcgtccaga cgaaatttac gccggtcaac ggacaggatc
    16621 atcggacgcc ctatgatgcg caggccgatc tcaccaccca ggtgaagcaa tcgttcggca
    16681 gttcgctcgc gcatctgcat accgactacc tcgactccta cgtgctacat gggccctacg
    16741 cccgccgggg gttgagtgac gccgattggg aagtgtgggg agcgatcgaa agtctctatg
    16801 cggcgggaac cacgcgaatg atcggaatca gtaacgtgtc ggcggatcag ctccggctgc
    16861 tctgtgaacg ggcgacgcat aaacccatgg tcgtccagaa tcggtgttac gccgccctgg
    16921 gctgggaccg agacgtacgg gagatttgcc gtgcgcacgg gatcatctat caggggttct
    16981 cgttgctcac ggccaaccgg gatatcttct ccgatcccga tgtgcgcacc atggctgcca
    17041 agtacggcat gggcttggcg caactggtgt tccggtttgc aatgcagatg ggcatgctgc
    17101 ccttgaccgg cacgaccgac cagcaacata tggccgagga tctccggtcc gatcagttca
    17161 ccatttcgac agaggacctc cagcggcttg aaacggtcgg gatgtagccg aatgccggca
    17221 tatcgccggt tctgaatcca tggacagcct gcatcaactg gaaatcatta tcctgctgct
    17281 cgcggtggtg ctggccctca ccacgatcgc gcagaagatc agcatcccgt acccgatttt
    17341 tctggtgctg ggtgggctgg tgctgggcgt ggtcccgggc ctgccaacgg tgacgctgca
    17401 cccggacctt gtgtttctgg tgtttcttcc gccgattctc tgggcggccg cctatttcac
    17461 ttcgttgcgg gagttccggc agaacctccg gccgatttcc ctcctagccg ttggcctggt
    17521 gctcgcgacg accgcggggg tcgcggtggt ggcgcatgcg ttgctgcccg gcatcggatg
    17581 ggcggaagcg atcgccctcg gagccatcgt ctcgccgccg gacgcggtgt cggcgactgc
    17641 catcgggaaa cgcctgcgca tcccgcgtcg tgtggtcacc atcctggaag gagaaagcct
    17701 ggtcaacgac gccaccgcgc ttgtgttgta ccgtgctgcc gtgggtgcgg tggtgagcgg
    17761 ctcattcctg ctcgggggaa cgctgctcaa ttttctcttt gccgcgctgg cggcactggc
    17821 gattggtctt gccgcggcct ggctcacccg ttgggcgctg tgtgtcacgg aggacggata
    17881 tacgcagatc ggcgtgacac tgctcgttcc ctatatcgcc tgggtcattg ccgagtcggt
    17941 ccatgcctct gccgtgttgg cctgtgtggc cggcgggatc acgcttcggc agtatttcag
    18001 cggcgcggtg gcgccggcca ccaggttgca ggctcgcgcc gtgtgggagc tgttgatctt
    18061 tgttctgaat ggcgtgattt ttattttgat cggattgcag ctgggagccc tccgggatgc
    18121 cgtccctgcg gggcagtacg gccctgtcgt attcgcgggg ctcatggtga gcgcgacggc
    18181 gatcgtcgtg cggtttctct gggtgccgct ggcggccctg atcccgcgct gcctgagcgc
    18241 gaccctgcgc gcgcgtgacc cgatgccgtc gtggaagggg ctgtttctca tttcctggac
    18301 cggcatgcga ggcatcgtca cgttggcggc ggccttggca cttccgatga cgacggggac
    18361 cggcgcgccg ttcccgttcc gttcagagat catcctgatc agctttgcgg tcatcctcgt
    18421 cacattggtg ctgcagggac tgtcgcttcc gccgattatc cggtggctgc gcctcgaaga
    18481 cgaagatgat ctggatcggg agcaacgcct ggcgcgcgaa catgcggcga ccgcagccct
    18541 ggcccgcctc gatgaggtca tgaccgagga ctgggtaccg agggagcaaa tcgaacgggt
    18601 gcgattgcgc tatcttcgcc ggctggaaca tctcaatcgg accgggttga atgaggagga
    18661 gcgtccggag ggaagtgttg aaaccgtcca gcgtttgcac catgaagtgc tcacagccga
    18721 gcgagtcgcc ctgatcgggc tgcgggacga cggcacgatc agcgatgaag tgttgcatca
    18781 cttggagcag gaactggatg tcgctgcgct gcatcagggc gtcggtgagc gtcgcgcggg
    18841 aaagggtaga atctgacatg ccgatccata ttgccgcagc ccagccggtc gatgcgccga
    18901 tcattgcgaa gttggtggga gacctgctgg cggaaattgt ggctgcgatc aagacgcccg
    18961 ccttcggatt ttcgttggct gagaccgaag cacgggctcg cgcctggctg gaggatggaa
    19021 gttatacggt gttgctggcg cggaatggag aaggtgctgc aggatttatc gccctggccg
    19081 aatgtcgtgc cctgtatgcg gagggacggt tcggcatcat cccggagttg tatgtctcgc
    19141 cggagcggcg gtcacaaggc gtggggggcg cgttggtgaa cgaggccaag cgtgtggccc
    19201 gctcgaacgg ttgggccagg cttgaggtca ccacgcctcc gttgccgcag ttcgaccgga
    19261 cgctggcgtt ttatgagcgg caggggttca gcgtgtccgg cggacggaaa atgaaagtgt
    19321 cggtgtgatc cggccagtgg ttcaggaaga acgaaccggt tgtggcatcg cggcagcggc
    19381 cgcgctggca ggggtgtcct atgcgcgtgc gaaggccgtg gcgaaatctt taggcattgt
    19441 cgcttcggac cgaaaactgt ggtcagagac agaatatgtg cgagacctgg tggcgcactt
    19501 cggcctgcga gccgcacgaa cacggcagcc gtttcgcgca tgggcgagtt tgccggatta
    19561 cgcgttgttg gccatcaagt ggcgcctgga gcaagggcga ccattctggc attgggtcgt
    19621 attcgtgcga gagaacgacc ggtcgtacgt ggtagattcc agcccgacat tgaaggctcc
    19681 ccttcggacc gacttcggac gtatgaggcc ggtgtggttt ttgactgtga cgatgaagag
    19741 gtagcgagtg aatattgcca ttattggagg cggaccggcc gggctgatgg ccgcagaagt
    19801 ggcgctggcc ggaggcgcgc aggtcgcact gtacgactcc atgccttctg tgggccgaaa
    19861 atttttactc gcggggaagg gcgggctgaa tctgacgcac tccgatccgt ccgatctttt
    19921 tctcagcaga tacggcgagc gtcgagcaca ggtcgctccc tggttggccg agtttggtcc
    19981 tgatgcgctc cggacctggg cacgggggct cgggatcgag acctacgtcg gctcatccgg
    20041 ccgcgtcttc cccacggata tgaaggccgc cccgctgctg cgcgcctggt tgcggcgctt
    20101 gcgtcaggat ggcctgacca tccacgtacg gcaccgctgg tgcgggtggg atgaaaagaa
    20161 aacccttcga ttcgagacgc cccatggtgc gacggccgta cgggcggatg ccgtgatcct
    20221 ggcgctaggt ggaggcagtt ggccgcaact cgggtcggat ggtgcctggg tcccggtgct
    20281 cgccgggcgc tcgatcgcga ttgctccctt gcggcctgcg aactgcgggt ttgacgtcgg
    20341 ctggacggag cactttcgaa cgaagttcgc ggggcatccg gtgaaaacgg tcggcgtggt
    20401 ggcaaaatct ccaacgggcg cggtgatgcg tcgcatggga gaattcgtga tcactgacac
    20461 cggtgtggaa gggggcgtca tttatgccgt ggccccgttt ctccgggatg agatcgaggc
    20521 caatggacga ggcacgctgc ggttggatct tgcgccggac cgtgaactgc cacgcctcat
    20581 caaagatctg tcgcagcctc gtggaaaaaa gacgatggcg acgcacctgc agcgccgcgc
    20641 gcatatcgat ggagtgaagg ccggattgct gcgtgaaatc gtttccaaag aggattttgc
    20701 cgacccgacc cgcctggccg ctgccatcaa agccttgccg ctcacgttaa cggcgccgcg
    20761 cccgttgcac gaagcgatca gtacggccgg gggagtgacc tgtgaggcgc tggacaagcg
    20821 attgatgctc aaagcgcttc cgggcgtatt ctgcgcggga gaaatggtgg attgggaagc
    20881 gccgacgggc ggctatctgc tcacggcttg ttttgccggt ggacggaccg ccggtgcggg
    20941 ggccctggcc tggctcgcgg agcgcgcaga gcgtgccaag gcataacgtg acgatcgacg
    21001 ggatggcctt ggtcgtgccg ggtattggca actcgggacc gcgccattgg cagacgctgt
    21061 gggaacagca acatccacgt tggcaacgag tgcagcaacg cgattgggat cgtcctgtgc
    21121 tgacggaatg gctgccggcg ctggagtcgg cgcttgccgg gatatccgcc ccggctgtgc
    21181 tgatcgctca tagtatgggc tgcttgctgg tcgcgcactg ggcgcaacag gcttcagttc
    21241 cagtgcgtgc ggcactgttg gtggcgccgc ctgatccgag gtctcccgcg ttccctgcgt
    21301 cagcgcgagg ttttgagaca gttccatctg gcaggctgcc ttttcccact ctcttggttg
    21361 ccagcagtaa tgaccctttc ggctctgtgg cctacgccaa gcaatgcgcg accgtttggg
    21421 ggagtgcttt cgccgaagcc ggcgcaatcg gtcatatcaa tgccgagagc gaaattggcg
    21481 catggcctgc cggtctcgtc ttgctcgaac ggttgctcgc gtcctcgtga tgtgccatac
    21541 ctgccacagg tctaaggctg caacttccta acaagtcttc ccgatccatc catctagatc
    21601 agtatgcgct ggtggccccg gtcggccagc gtcccggtgg tgagattcca gtcgaagagg
    21661 aagatggcgg attgttcgca cgagagcgac aggagccgtt tcccattgtg agcgaccaca
    21721 ctttccgctt ttctaaaatc gagagtgcgg tcatgccctg tgcgattgca gacgaacaac
    21781 ggtagtcccg tgtctttcgt gcagcgctcc cactcgtcgt tcggaccgtg gagtcccggc
    21841 gcccaggcag ccgaagacac gaggacagtg gccccctgcg ctttcaagct cttggcaatg
    21901 cccggggtat aggcatcggc gcagatgagg aggcccacgc ggccgaatgg cgccagacga
    21961 aatgcagtcg cgtctgtgcc gggggtggac caggcctcgg acccgacgcg caatgcgttg
    22021 atctttctgt ggctgcccgc aatgcgacca tcggggccga tcgcaaatac cgtattgtag
    22081 agctgcccgg atcgtggctc tttctccggg tgcgacagga atagcgtgac tctgagtcgt
    22141 gcggcgaggc ggcagattct gctcatccaa tggtccggct gaggctcgat ccactccgtc
    22201 ccgagcgtat cggcgaaggt gtacccgctg actgccagtt ccggggtgat gatccaactc
    22261 gctcctgaac aggccgccct ggtgatggcc tgggtgagga ggtggcggtt ttgcgcgatc
    22321 gctccgggaa tcggggcgag atgtaagcag gcgatgcgaa gtagagatcc ggccatcggg
    22381 tcccacgtca tgtggtggct gtgccaatac tgttgggcgt ggagtgtacc atagcccggg
    22441 tacctgagcc tctcccgggt gctgtgactt gtgtccgcct cgttcccctg cgtagagtgg
    22501 aacgaaggag ctggccggca tgaatcatcc tatcgtcccg ccgtcacgaa aacggttagg
    22561 tcagtggctc ctgctgctgg tgcttctgct ggcgctcctc tgtgtggcga cgatcctggc
    22621 gttgccatgg ctgctgaatc gtccggcgag catggcggcc ttgcttcagc aggtcgaagc
    22681 gcggacaggg catcggattt ccttcgagca atcgcatgtc gagatctttc cctcgccccg
    22741 tgtgacactg atgcagcccc ggctctacgc cgccacctct cccgatccac tgctgttcgc
    22801 ggagcgggtg gaggtcgcgc tgcagtggct gccgcttctg gagggatccc ttgtcgtcaa
    22861 agatctggtc atcgaccggc ctcgtgtgac cctccgccgg tcgccaaatg ggaattgggc
    22921 gctgggcaac gagcattccc ccggccctga ttcagagccc accccgccgt ttgcgttgtt
    22981 gcaggtcgtg aggaatctgc ttgtggttca agggatggtg acgctcgtcg atgaatcggc
    23041 ttccccgtcc acttctctca acatcatcgt gatgcaggct gcgttatcga gtgaaatgtt
    23101 gggccgccgt gcgaagctgc atctttcagg agagctcccg caggcaggcg ggcgcgcggc
    23161 gttcctctgg gaagggtcca tcacgcaaca gggcgacgaa gatggaatac aggcagaagg
    23221 ggatctgcga ctccatcagt tgaatgtgcg tcaagtgttg tctgcctgga tgggcgacgg
    23281 ccggatcacc gatggtttct cgcagcccgc acaattgacg gcgcacgtgc ggtggaagcc
    23341 gggcgcggcg ggatatgatc tgcttgccga cgatgtgaga gccgagttgg cggatgtgtc
//
LOCUS       DAOQEW010000042        23211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_535_length_23211_cov_7.959579, whole genome shotgun sequence.
ACCESSION   DAOQEW010000042 DAOQEW010000000
VERSION     DAOQEW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23211
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_23211_cov_7.959579"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1216)
                     /gene="flhB"
                     /locus_tag="PLS35_06715"
     CDS             complement(134..1216)
                     /gene="flhB"
                     /locus_tag="PLS35_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNI18384.1"
                     /translation="MADSAQNRTEQATPKRKAEARSKGQIALSRDAAMAVALLGSFAA
                     LYWMTPGIMERLRHSLQRWLSQSMEDSTLRALSLDHLHLILRQIGIDVFVMLGPVVAG
                     IAVVGVGANLMQTGFLWRKDGLSIDWSRISPVGGFSRLFSVRALSELVKSWLKILAIG
                     SVGYLTIKEDMLQFLPLTQFGMETLLPTVGWATFKAALMMSGAALVIGALDYGYQRFE
                     WERGLRMSRDEIKEESRAAEGDPGLKAKIRSTQREMSRKRMMAAVPTADVIITNPTHL
                     AVALKYDSKAMGAPIVVAKGAGFIAEKIREIGRQHGVMIVENKLVARTLYKLVEVGRE
                     VPEDLYRAVAEILAFVYRVRGKLPPA"
     gene            complement(1209..1997)
                     /gene="fliR"
                     /locus_tag="PLS35_06720"
     CDS             complement(1209..1997)
                     /gene="fliR"
                     /locus_tag="PLS35_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNI18385.1"
                     /translation="MNAAQTLHLALPQFQSFSILLVRIAGIISVFPILNTLTIPMPVK
                     AGLVTMLGLVLAPIIQLPSFPNDPALVVAGIGSEFLIGLTIGLAVRLLFSGFQVAGEL
                     VGTQMGFSAIQMFDPMSHQNAPIIAQFQLTLGSLVFLSLNAHLMVVHAIGMSYEFVPP
                     FGAKLSDSIATDIIHLAQNMLGVALKMAAPVFATLLIVNTVLAILGRAVPQMNVFVLS
                     FPITIGAGLFAMSLAMPFTLSLFEKEFENLVETILSLLKGLGHG"
     gene            complement(2013..2282)
                     /gene="fliQ"
                     /locus_tag="PLS35_06725"
     CDS             complement(2013..2282)
                     /gene="fliQ"
                     /locus_tag="PLS35_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNI18386.1"
                     /translation="MTIETVTEIGRQAIETAMLVSAPVLGLSLVVGLMVSTFQAMTQI
                     NEATLTFVPKVLAVFAATLLFLPWMMGVLIAFMTHIITSIPTLIH"
     gene            complement(2294..3094)
                     /gene="fliP"
                     /locus_tag="PLS35_06730"
     CDS             complement(2294..3094)
                     /gene="fliP"
                     /locus_tag="PLS35_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960008.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNI18387.1"
                     /translation="MPDDNLTSLQQAKKGGAGTVPRCMGWVGLVLLVVLAGASSVHAE
                     GPSLTLDLGQSGTKQTAVVLQILALLTVLSLAPALFIMVTSFTRMVIVLSFLRQALGT
                     QQVPPNQVLISLALFLTMFVMAPVGQAVYKDALQPLLAEQIGYEDAWNRGIQPIRGFM
                     LKQMRDKDLELFLELSHSPKPAQVDQVPTHVIIPAFVLSELRISFQIGFLLYIPFLIV
                     DMVVASVLMSMGMMLLPPVMISLPFKLILFVLADGWYLVVGSMVKSFQ"
     gene            complement(3081..3449)
                     /locus_tag="PLS35_06735"
     CDS             complement(3081..3449)
                     /locus_tag="PLS35_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNI18388.1"
                     /translation="MEIWESVVRMVSALGIVLALILGLLAIVKSRVGQRFLPVGGAPL
                     IKVLGSSSLGARKQVMLVAVAGEVLILGTTPTDLVSLGKVTDPEQVKRLLLEASSNMA
                     LSPAQAVGSEYREETLHAGR"
     gene            complement(3506..3874)
                     /gene="fliN"
                     /locus_tag="PLS35_06740"
     CDS             complement(3506..3874)
                     /gene="fliN"
                     /locus_tag="PLS35_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNI18389.1"
                     /translation="MGNGDTTPQADASSGADMSPQATSFPPVDNQGAVPANKNIDFVL
                     DIPLQVTVQIGSTRMLIRELLQLGQGSVIELNKLAGEPMEVLVNNKLVARGEVVVVNE
                     KFGIRLTDVVSPNQRIQQLG"
     gene            complement(3904..4875)
                     /gene="fliM"
                     /locus_tag="PLS35_06745"
     CDS             complement(3904..4875)
                     /gene="fliM"
                     /locus_tag="PLS35_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNI18390.1"
                     /translation="MDKILSQEEIDALMGGVMSGEVETAPKEEEEPGGIKTYSLTSQE
                     RIIRGRMPTMEMINDRFTRQQSVSWTSVLREKIEFSVLGTQIIKFGDFIQKVPVPSSF
                     NLFAMEPLRGNGLYIMDAMLVYLIVDFFFGGKVQTHVKPEGREFTTIQLRLIKKLVQQ
                     ALVDLEKAWQAVMPVKLNYLRSESNPQFAMVVTTSEIVVVVTLQVHLGDITREMFIAY
                     PYSMLEPIKEKLYSGLFADNVEQDSSWGSRFKDSLQECDVAVTVQLGTAKINVEDLMN
                     FSPGDVLMLDQHPGDPMLCLVEGDPKFHGQPGVFKGNHACRVTKVLS"
     gene            complement(4897..5436)
                     /locus_tag="PLS35_06750"
     CDS             complement(4897..5436)
                     /locus_tag="PLS35_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNI18391.1"
                     /translation="MSEAAEEKAPAAPAGIPMKMVIIIVGGVLILALGGAFAMMKMMG
                     GSSAEKTQAEGGGEKAAAHGAAEEKSGGEHGAVADASAIADLDPFIVNLADSPEIRYL
                     KVTMKVEADNAGTVEQIKARTPQIRDAILVLLTSLDSATARSPQGKHKLRDDITERIN
                     GLLPKKSVKSTYFTEFVIQ"
     gene            complement(5769..6992)
                     /locus_tag="PLS35_06755"
     CDS             complement(5769..6992)
                     /locus_tag="PLS35_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNI18392.1"
                     /translation="MGILTSMFTAVTGLSSYGNAMGVIGNNIANVGTAGFKSSRATFS
                     DLISSSMAGGAGSDQIGLGVYLNDVQTNFSQGSMTTTGNTFDLAIDGNGFYLVRNASG
                     SNFYTRAGQFKVDSVGQVVDASGALLQGYQADTLGNITSTIGGITLTSSAVPPQSTST
                     ATILGNLNANATAPTAAFATTDTTSYNFSTGMTFYDSLGNSHQMQLYFRKTAANAWGV
                     YSQIDGGAATAQTNMTFNASGAVTAGGTQTITAALTNGATTPQSISVNLASLTQFGAA
                     SGVQSQTQNGYSSGTLDRISVDKEGKVMAQFTNGQTRALAQVVLSRFTNPDGLANVGD
                     NHFLETVESGAALAGAPTINGLGRVLSGTVEQSNVDLGKEFVDMIITQRAFQANSRAI
                     TTSDEMLQELVNLKR"
     gene            complement(7066..7356)
                     /locus_tag="PLS35_06760"
     CDS             complement(7066..7356)
                     /locus_tag="PLS35_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNI18393.1"
                     /translation="MDISKVITQTPAADPSTPTTEGPKPLGKDDFLKLLVTQLQHQDP
                     LKPMENEAFIAQTAQFSQLDQMTKMVSLMEKSVALQESAQNKTTQTGTTAVA"
     gene            complement(7368..8963)
                     /locus_tag="PLS35_06765"
     CDS             complement(7368..8963)
                     /locus_tag="PLS35_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNI18394.1"
                     /translation="MATDMQPVFNGFPSRSSQGIQDARDRQPAVHGSDPSRNFESVLG
                     RAEARQTPPKHEAPTPRSGEKAGLHARQVSRSISDSSSQPSERSSVAPSDDHSSNRCE
                     CAGEKAQPSTDKSEEKSTTKESKKKDPGQDAVSQDMLLASMMVPTAQAAPPEQQTPQA
                     EAQSDSHSETSVNAAAAISSDRAEDGSASVTTLVAATSEAGAGTQEVLAGQSDPKADA
                     GKDAAQAKERAENGNEMVQTTAKELPSLAGDNSSPDARVEDRTDSTAAARTDADRMNP
                     AIAAMTSSKQNPHMAEQGPSEGADRSAILDALAQGASLSEGQAGSSTGRFLEPDGQNL
                     SDSSGGTDRSLPNQVPSVEQNARPAFMDRINDVHQSTPSVNDSSSGRSESGQTTAVLR
                     ASQSERLGELRGMAPVSQSVTLDLDPLDMGPLRVRVMMSDQTVHAHIRTEHGELGQGL
                     LQQGQSLESSLRTSGLEMGMLRVTVDQQQGRGDNAWMFQQQQQQQNRPSSPGLSQTVA
                     REDERGARGERGAGSNERVSFFA"
     gene            complement(9160..9792)
                     /locus_tag="PLS35_06770"
     CDS             complement(9160..9792)
                     /locus_tag="PLS35_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18395.1"
                     /translation="MMETGSRSEQGRCVGVVALIAFGAVFLTLITTAHATEEVKPAAV
                     PQKEETKPESVKKDVQGPATSAPREIYEMLEQRKRLLDKRETAVRGSETHLLELKAEL
                     EQIVTRHEQAVEAEKKRRQAAQAKGAGEAEKAKTTAKATPAVNVNQAQLAKIYEAMPA
                     EEAAARLERMPERKALEVLRLLKGKSAGAILAEVKPERAARLTEQLLTTP"
     gene            complement(9789..10229)
                     /locus_tag="PLS35_06775"
     CDS             complement(9789..10229)
                     /locus_tag="PLS35_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HNI18396.1"
                     /translation="MNVTVLRAYAVQLEEVAKLELAELSHALQQTVNRMAMLESRAKE
                     DADRYLHQVRAGSTVDQVYGHLDAMDQAIAARKGLEQALATEQARMEQKRAELLEAMQ
                     YRKKLDLLDARAALEIRRRRERQDQQLLDERAWRRSPLHKGERA"
     gene            complement(10226..11539)
                     /locus_tag="PLS35_06780"
     CDS             complement(10226..11539)
                     /locus_tag="PLS35_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNI18397.1"
                     /translation="MPVKLSARLEHVEPLSVTGRVAQAVGIVIEASGPFTSVGEVCEI
                     TREGGHGSVMAEVVGFREDRVLLMPLGEMQGIGPGSRVVMKGHVASVPVGPQMLGRIF
                     DGLGHPLDGLPALRSDVRVPLYAPPLNPLHRARITQPVDLGIRAINALLTCGQGQKIG
                     VFAGSGVGKSVLLGMISRYTQADVRVIALVGERGREVKEFLERDLTPEARARSIVVVA
                     TSDQPPLVRIRGALVATAIAEYFRDCGKHVLLMMDSLSRLAYSQREVGLAIGEPPTTK
                     GYTPSVFAVLPKLLERVGPAQSGGSITGLYTVLVDGDDLNDPVADAVRSILDGHIVLS
                     RELAARNHFPAIDILQSTSRVMRDIVTPAHYAAARLVLERTALYRRSEDLITLGAYKP
                     GTSKDLDKAVSAHDGITAFLRQETKQAVGLPQSIDALLALAGTIQ"
     gene            complement(11523..12572)
                     /locus_tag="PLS35_06785"
     CDS             complement(11523..12572)
                     /locus_tag="PLS35_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18398.1"
                     /translation="MGSAAAGTGVSMAASPESEKELSPRAVLIVDDEPSICLLLAEML
                     ESTGHPVLTAGSVPEALSHLRTRSVAVVIVDVQLGGADGIAFLQQALDIDNRLLGIVM
                     TGHGNIELAVRAMKSGAADFLTKPFQVELVRLAVSRLLELYRLRQENTVLTRTLIRSG
                     NIQLRPVPLADFSRGNRPFGTEDLTEFERGVAEGEKRSTERVAAARQREQSLVASLMT
                     RLEETWQSLHETVEEEIASLSFMIAHKVVRDVVTEKRDLIVTQVRTALAHLHESGLVR
                     VRVHPSDLTVLESARTALSQTPHGLLTLKFETDPSISPGGCLVQAQSLLIDATLDTQL
                     LRLGEALRKRDAGEA"
     gene            complement(12573..13574)
                     /gene="fliG"
                     /locus_tag="PLS35_06790"
     CDS             complement(12573..13574)
                     /gene="fliG"
                     /locus_tag="PLS35_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNI18399.1"
                     /translation="MAINKELSGAQKAAILLRAIGEEAAAAVMKSLDPKDIRKLGEFM
                     KETANITKQEEDSVIAEFEQASSSGEVQFEGREFMEAILKKALGPEKAARMMESLNTK
                     TYPGIDALKWVDARTVAQILKIEHPQTIAVCLGQMEAEQASAVLALLPVHLHADVSLR
                     LATMQEVQPEVLAELSDSLQEILSASMGMSSMSVGGAELMADILTRVDKNTEGAIMAK
                     ITERDQALAESIRALMFVFDDLVDLDSRGMQELMKEISKEDLPIALRGATPEIKDKFL
                     KNMSSRAAEMLKEDMETRGPVKVSDVEKSQQNILKVCRKLEEEGRIVIGGGGGEELL"
     gene            complement(13580..15139)
                     /gene="fliF"
                     /locus_tag="PLS35_06795"
     CDS             complement(13580..15139)
                     /gene="fliF"
                     /locus_tag="PLS35_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNI18400.1"
                     /translation="MFSKFSQFTINQRFIILLALAGSIAGLVAVTLWTQQPDMQVLFA
                     NLATDDAAGIIDKLKDAKVPYETTNGGTTILVPNAQVHDLRLEMAGQGLPHGGGVGYE
                     IFDRTTMGMSDFVQKLNYRRALQGELARTITQMPEVERARVHLAIPERRLFATEQDRA
                     RASVVVSLRPNQNLSKAQIQGIVHLVSSSVEGLQARDVTVVDGHGNLLSNSSSDESAG
                     LSGTQMEYQRTLEKNIETRIQTMLERIVGVNKAVVRVSSVLDFRKIETTEERYDPNGQ
                     VVRSEQRGQEKSSGVNGTTGGVPGVESNVPGGAEGEGGQTSSNNNQTKNETVNYEISR
                     TVSRIVEPTGTIKKLSVAVLVDGTYEGGGKAAEGAAEQPKKYVPRSEEEMKRIEEIVK
                     KAMGYSTERQDQVEVVSIQFGLGVEEPVGAGVEAAADSTRAWMPYIRYAVGGLLFFLI
                     LFMVVRPLMVMLVQSAPVEAVGETPALPASVGQVEAAISGKQPGQILDMAKNNPANTA
                     VVVKQWLKSNA"
     gene            complement(15187..15498)
                     /gene="fliE"
                     /locus_tag="PLS35_06800"
     CDS             complement(15187..15498)
                     /gene="fliE"
                     /locus_tag="PLS35_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNI18401.1"
                     /translation="MSDLHIAGANLIKPIEAPGLQQAGQTGEAGGANFLGSLKEAIGH
                     INDAQAGASQAVESLVTGQNTNIHQTMVALQQADASFQLMMQVRNKLVTAYEEIQRMQ
                     I"
     gene            complement(15501..15950)
                     /gene="flgC"
                     /locus_tag="PLS35_06805"
     CDS             complement(15501..15950)
                     /gene="flgC"
                     /locus_tag="PLS35_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNI18402.1"
                     /translation="MDLSDSLAVSVSALDAQRHRLNVIASNLANAQSTKTSTGGPYRR
                     RDVVFQAAPVSSAFQKAFTQVSKGSGRHALDGVKVARVIEDQKPGQTVYDPRHPDADK
                     KGFVTMPNVNVMEEMVNMIGASRAYEANVQAVNATRTMWNRALEIGR"
     gene            complement(16012..16419)
                     /gene="flgB"
                     /locus_tag="PLS35_06810"
     CDS             complement(16012..16419)
                     /gene="flgB"
                     /locus_tag="PLS35_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNI18403.1"
                     /translation="MTIFDRTMQLLERSLDLRGARQQVIAANIANEETPKYRATDLNF
                     GQALANAQQGKLPIALVSTHQQHIGPKGSGFQRVQGRIEEVPAGDLPLDANSVNIELE
                     MAKMSDNAQQYNTSASIISMRFKTLLSAIREGR"
     gene            complement(16590..18008)
                     /locus_tag="PLS35_06815"
     CDS             complement(16590..18008)
                     /locus_tag="PLS35_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18404.1"
                     /translation="MSAQPALQGPSASADEARMVLIVDDEPSMRTALSETVRRMGYQV
                     RSAIDGADAIEQVERLKPWLVVTDLKMPRLNGLELVKALKQKAPQTFIILMTAYGTVE
                     TAVEAMKYGANDYILKPFSTDLLERVILNLQASAEPDEQDAPAALEARAILTQDPGMI
                     RLLTTLEGVAASQATVLISGESGTGKELLARYIHARSPRAHRPFVALNCAALPDSLLE
                     SELFGHERGAFTGAIQKKLGKFEMAHTGTLFLDEISEMNLGLQSKLLRVLQEREVDRI
                     GGRDPVPVNIRVIATTNRSLYHEVTQGRFREDLFYRLNVFPVTVPPLRERPGDIPLLA
                     RHFLRSSAQRNGLTVPTLSERAIADLQQRAWKGNVRELENVMERAILVTTGNAVDVDH
                     LMPGDGTVPIQPVETVETLVAPSAHGSLWEMERDLIFKTLARVKENRTHAAKELGISI
                     RTLRNKLREYRDLGYQVEAEKP"
     gene            complement(18005..19294)
                     /locus_tag="PLS35_06820"
     CDS             complement(18005..19294)
                     /locus_tag="PLS35_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18405.1"
                     /translation="MIRQEGDTTNNDLLTRAFRDFDQAATVLQQSYDALTTRLQQMDL
                     ELAQTNASLREHLRETEEMRAHVTAVLESLDTGVIVADSQDVVVRCNHSTEQLLGVPQ
                     AQLRGRQASALLAEIRKDHGEYPLVLPSGVTIALSQTDLTDESGNPIGKLVLIHDVTR
                     IRQLEDRLQRRNRLEAMGQMVGSIAHEIRNPLGSVELFASMLRKDLRDQPQLRSYAEH
                     ISMAVQSMDQLLSNLLVYTRPDCSRLGWHDTEVLIREVLTLASHAIPPDTVDVRCQMH
                     PLVPQLWCDGPKMKQVLLNLVLNAVQAMPKGGTLTLTACPSPPEIQEVPAVHITVSDT
                     GTGIPAELQSRVFDPFFTTKDQGTGLGLAIVHALVEAHHGRIDVESRPGHGTSFVITL
                     PRGPRQPSSTPVSLVTVSAPSERSRHITSITEEKRYE"
     gene            complement(19291..21327)
                     /locus_tag="PLS35_06825"
     CDS             complement(19291..21327)
                     /locus_tag="PLS35_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18406.1"
                     /translation="MMAEPSELVRGALPDPGPRFERLPEIPEWKQFEQGVGLYHQGQY
                     LEARLHFTNMLRDRRESPLRSSIQAFLAESTLQSHDPGVRPLDIIDQYKTLMRENPQS
                     TNAKRAAWRIGDVYRIEGWFQEAQIAYQHALSLSEIDSFDANRAMLGLAYVQRGFKNW
                     KESTQTFDHVLKRTVDPTLLVSASLGEAHSLYRLGRMKDADALYESISGRWPAALRSD
                     AFALLRYADTAGEAKRGPVMREQLLHFYNLYPNRPETPFVLTYLADSYKEAGRWAESS
                     IFYAALLSQYPEAPVAATARLRYADVQEHLDPEGEEINLRHTISAHLDHVPLKAGEML
                     SPRRLFEQSAKDNEGSIVGSEALFHLGETLERAGKPEGAVRAYEQVVLRSGKFDNDPW
                     PEKGGVQLVRFLRPRLEAALKAEDDFELINIFHRHGPFADRLYAGTELLLKIAEAHRR
                     LGFPVESARLFQSLVRDAKAEKFHEAALMGLAYSYMEQKDMRAARSVFERYRLEFPTG
                     RFSGEALRGILNSFEGEGNLAALLKLGKQWLAHNPRHHDRTMVQLKLAAVLAQSKQPA
                     EAAAIYDGLIKAGQELPAADLLRHAEVLARLNRRSQALAMYKEAVVAGLELEQEAWAQ
                     LQMVQLARGEKREDFAKNGLRALSVNPDSLVRRMAAMLDNELPEPPVDKRGKKP"
     gene            complement(21625..23040)
                     /locus_tag="PLS35_06830"
     CDS             complement(21625..23040)
                     /locus_tag="PLS35_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18407.1"
                     /translation="MSQSHILVIDDDPAVRQLLAETLTSEGHQVTVMSSGLDGVEAVK
                     DQPVHVVLTDLQMPGIDGLETIDRISKIDSKVIAIVMTGYGTVDYAVRAMKAGAFDFI
                     TKPFEPDTVAVVVRKALDVYKLKQENHLLRKAVRDQYRLEHLVGTSAPMRMVLDFVEK
                     VADSDSTVLIEGESGTGKELIARMLHFNSMRRDRPLVPVNCGAIPETLLESELFGHEK
                     GAFTGAAHTRLGRFELAHGGTIFLDEVGEMSLPLQVKLLRVLQERCFERVGGTRTINV
                     DVRIIAATNQDLAQAVQERRFRQDLYYRLHVIPIHIPPLRERRSDIPLLVNHFIAQFN
                     QLRRTEILGMEPDALVRMTEEEWPGNIRELENMIERLCVLKKRGMITLADLPERALKT
                     AGGKAVEAPEQFIRFSEDGINLTKELEHYENRLIGEALRKANGITSRAAQLLQVNRTT
                     LVEKLKRKGFDPKSHGYSIQN"
BASE COUNT         4631 a         6924 c         6465 g         5191 t
ORIGIN      
        1 cgcggcagcg ggacgagcat caccatcaga atgccgacca cgcccacgga catcagaatg
       61 tccggatgct tcacgaggga tgtgctgggt atcgaggtat tgtccttcgc catctccgct
      121 ccctaccggc tgactatgcc ggcggcaatt tgccacgtac gcgatacaca aaggccagaa
      181 tctcggccac ggcacgatag aggtcttccg gcacttcccg gcccacttcg accaacttgt
      241 acaacgtcct ggccaccagc ttgttttcga cgatcatgac gccatgctga cgaccgattt
      301 cccgaatttt ttccgcgatg aatccagcgc ccttcgccac gacaatcggt gcgcccatcg
      361 cctttgaatc gtatttcaat gcgactgcca ggtgggtcgg gttcgtgatg atgacgtccg
      421 ccgtgggcac cgcggccatc atgcgcttgc gagacatctc gcgctgggtg ctgcgaatct
      481 tcgctttgag cccgggatct ccttccgcgg cacgactctc ttccttgatt tcgtcgcgcg
      541 acattcgcag accacgttcc cattcaaagc gttgataccc gtagtcgagg gcaccgatga
      601 ccagagcggc gccgctcatc atgagtgcgg ccttgaaggt cgcccacccc accgtgggca
      661 gcagggtttc catgccgaac tgggttagcg gcaaaaattg gagcatatcc tctttgattg
      721 tgaggtagcc gacgctgccg atcgcgagga tcttgagcca ggacttcacc agttcgctca
      781 atgcgcgcac cgaaaacagc cgcgaaaagc ctcccaccgg actgatacgc gaccaatcaa
      841 tactgaggcc gtcttttctc cacaggaagc cggtttgcat caagttggcg cccacaccta
      901 cgacagcgat tcccgccact accggtccca gcatcacaaa cacgtcgatt ccgatctggc
      961 ggagaatcaa atgcaggtgg tcgagactga gtgcccgaag tgtcgagtcc tccatggatt
     1021 ggctgagcca gcgctgcaag gaatggcgga gtcgctccat aatgccaggc gtcatccagt
     1081 agagcgccgc gaaacttccc aataaagcga cggccatggc ggcatcgcgg ctgagggcga
     1141 tttgaccttt cgatctcgcc tctgctttgc gtttcggtgt ggcctgttct gtgcgatttt
     1201 gggcgctgtc agccatgccc caaccccttc aggagactca agatcgtctc caccaggttc
     1261 tcgaattctt tctcaaacag cgacagcgta aagggcatgg ccaaactcat ggcgaacagg
     1321 cccgccccga tcgtgatcgg gaagctcagg acaaagacgt tcatctgcgg cacggcgcga
     1381 ccaaggatgg cgaggaccgt attgacgatc agcagggtgg caaagaccgg cgccgccatc
     1441 ttgagtgcga cgcccagcat gttctgggct aaatgaataa tgtccgtggc gatgctgtcg
     1501 gacagtttgg cgccgaaggg cggcacaaat tcgtaactca taccgatggc atgcacgacc
     1561 atcaggtgtg cattgagcga aagaaagacc aacgagccga gcgtcagttg gaattgcgcg
     1621 atgatgggtg cgttttggtg gctcatcgga tcaaacatct ggatcgcgct gaaccccatc
     1681 tgcgtgccga ccaattctcc ggccacctga aagccggaaa acaagagacg gacggccagg
     1741 ccgatggtca ggccgatcag gaattcgctg ccgatgcccg ccaccaccag ggcgggatcg
     1801 ttcggaaaac tgggcaattg aatgatcgga gccagcacga gccccagcat tgtcactaac
     1861 cccgccttga ccggcatcgg gatggtcaac gtgttcagaa tgggaaagac gctgatgatg
     1921 ccggcaatcc tgaccaacag aatcgaaaac gactgaaact gtggcaatgc gaggtgcaac
     1981 gtttgcgcgg cgttcatgct gtggacgacc tatcagtgaa tcaacgtcgg aatgctggtg
     2041 atgatatgag tcataaatgc gatgagcacg cccatcatcc acggcagaaa caacagcgtc
     2101 gccgcaaaga cggccaacac cttcgggacg aacgtgagtg tcgcctcatt gatctgcgtc
     2161 atcgcctgaa aggtactgac catcaagccg accacgaggc tcaggcctaa aaccggtgcc
     2221 gacaccagca tggcggtttc gatcgcttga cgtccgattt ccgtgaccgt ttcaatcgtc
     2281 atgtgcagcc atcctattga aagctcttca ccatggagcc caccacgaga taccagccgt
     2341 cggccaggac gaagagaatc agcttaaacg gaagagaaat catgacgggt ggcagcagca
     2401 tcatgcccat ggacatcagg acgctggcca cgaccatatc gacgatgaga aagggaatgt
     2461 agaggaggaa gccgatctga aacgaaatgc gcaactcact gaggacaaac gccggaatga
     2521 tcacatgggt cggaacctga tctacttgcg ccggtttggg cgaatgggac aattccagga
     2581 aaagttccag atccttgtcc cgcatctgct tcaacatgaa gccgcggatc ggttgaatac
     2641 cgcgattcca ggcatcttcg tagccgattt gttccgcaag cagcggctgc agcgcatcct
     2701 tgtagaccgc ctgccccacc ggagccatca caaacatggt gagaaagagg gccaaactga
     2761 tcagcacctg attgggcggc acctgttgcg ttccgagagc ctggcgaagg aaggagagca
     2821 cgatcaccat gcgcgtgaac gaggtcacca tgatgaacaa agcgggcgcc agtgacagga
     2881 cggtcaacag cgcaagaatc tgcagcacca cggcggtctg tttggtcccg ctctggccga
     2941 gatcaagcgt cagcgatggc ccctctgcgt gaaccgatga cgcgccggcc agaaccacga
     3001 gcaggacgag accgacccat cccatgcatc gggggacggt tcctgcgcct cccttttttg
     3061 cctgctgcag agatgtgagg ttatcgtccg gcatgtaacg tctcctcgcg gtattcagac
     3121 ccgacagcct gcgccgggga gagagccata ttcgaggagg cttcaagcaa cagtcgcttt
     3181 acctgctccg ggtcggtgac ttttccaagc gacaccagat ccgttggcgt ggtaccgaga
     3241 atcagaacct cgccggcgac ggcaaccagc atgacctgct tgcgcgctcc cagcgaactg
     3301 ctgcccagca ctttgatcaa tggagcgccg ccgaccggaa gaaagcgctg gccgaccctg
     3361 gatttcacga tggccaacag tcccagaatc aacgccagga caatgcccag cgccgagacc
     3421 atgcggacca cactttccca aatctccatg gaacgatcga ctttctgctc gctcgcaccg
     3481 gcggtctcgc cgaatgacgc ggcgcctatc cgagttgctg aatgcgctgg ttggggctca
     3541 ccacatccgt caagcgaatg ccgaacttct cgttgaccac cacgacctcg ccgcgcgcca
     3601 ccaacttgtt attgaccagc acttccatcg gctctccggc cagcttgttc agctcgatga
     3661 cagacccctg tccgagctgt aacaactccc gaatcagcat gcgcgtcgag ccgatctgca
     3721 ccgttacctg cagcggaatg tcgagcacga aatcgatatt tttgttggcc ggtaccgcac
     3781 cctggttatc gaccggggga aatgaggtcg cctgcgggct catgtccgcg ccgctggagg
     3841 catccgcctg cggggttgta tcgccattgc ccatgcgcaa atctccttta cttccggcgc
     3901 gacctaactc aacactttgg taacgcggca ggcatgattg cctttgaata caccgggctg
     3961 cccgtggaat ttcggatcgc cctctaccag acacagcatc ggatcgcccg gatgttgatc
     4021 gagcatcagc acatcgccag gactaaagtt catcaggtct tccacgttga ttttggctgt
     4081 ccccagttga accgtcactg ccacgtcaca ctcctgcagc gaatccttaa agcggctgcc
     4141 ccagctgctg tcctgttcca cgttgtcggc aaacaggccg gagtacagct tttccttgat
     4201 gggctccagc atggaatagg gataggcgat aaacatttcg cgggtgatgt cgccgagatg
     4261 cacctggagc gtgaccacta cgacaatttc cgacgtggtc acgaccatgg cgaattgagg
     4321 gttgctctcc gagcgcagat agttgagctt cacgggcatg accgcctgcc atgccttctc
     4381 caggtcgacc agcgcctgct gaacgagttt cttgatcaac cgcaactgaa tcgtcgtgaa
     4441 ttctcgcccc tccggcttca cgtgcgtctg cacttttccg ccgaagaaaa aatccacgat
     4501 caggtacacc agcatggcgt ccatgatgta gagcccgttg ccgcgcaacg gctccatggc
     4561 aaacagattg aaggaggacg gcaccgggac cttctggatg aagtctccga acttgatgat
     4621 ctgcgtgccg aggacgctga attcgatctt ctcgcgcagc acgctggtcc aggagacaga
     4681 ttgctggcgc gtgaagcggt cattgatcat ctccatcgtg ggcatgcgcc cgcgaatgat
     4741 ccgctcctga ctcgtcagac tataggtttt gattcccccg ggttcttctt cttccttcgg
     4801 cgccgtttca acctcgccgg acatgactcc gcccatcaag gcatcgattt cttcctggga
     4861 gagtatcttg tccatgatgt tgctgacgtc gcgtggttac tgaatcacga attcggtgaa
     4921 gtacgtcgac ttgaccgatt tcttgggcaa caggccgttg atccgttcgg tgatgtcgtc
     4981 gcgcaactta tgctttccct ggggagaacg cgcggtagcc gaatccaagc tggtcaacaa
     5041 cacaagaatc gcatctcgaa tttgcggtgt ccgcgctttg atttgttcga cggtgcccgc
     5101 attgtcggct tccaccttca tggtgacttt gaggtaccga atttccggtg aatccgcgag
     5161 gttgacgatg aagggatcca gatcggcgat ggcgctcgca tcagcgacgg ccccatgctc
     5221 gcctccagac ttctcttcag cggcgccatg cgcagccgcc ttttctcccc caccctcggc
     5281 ctgcgtcttt tcggcggatg acccgcccat cattttcatc atcgcaaagg cgccgcccaa
     5341 cgcgaggatc aacactcccc cgacgatgat gatgaccatc ttcatgggaa tacctgccgg
     5401 tgctgctggg gccttttctt cagctgcttc tgacatacaa ccctcctgcg gacagtgttt
     5461 gccggtgccg gtcagttgtg caatgcatat gccacgtgcc gcacagagca gccctcccgc
     5521 ctgtcacaac acaggtcgca gatcgcgtga taagacaatg atttggagtt agtcactctc
     5581 ccgcgcgagg acacgtcgcc tgagcggcat acgtgttgtc acggtagtga cgcgggatct
     5641 ctgggggccg acagaatgtt gacgagaggc agaagacgaa gagccgcgag gatgagcgct
     5701 tcaactaggt agaaattgcc gagttgagcc ccggcgacgg gattgcaccg cgccggggcc
     5761 ctcaccgctt accgcttgag attcacgagt tcctgcaaca tttcgtcgct ggtcgtgatg
     5821 gctcgggagt tcgcctgaaa ggcgcgctgg gtaatgatca tgtccacaaa ctctttgccg
     5881 aggtctacat tggattgctc cacggttccc gaaagaaccc ggcccaatcc attgattgtc
     5941 ggggcgccgg ccagcgcagc accggactcg acagtctcca gaaagtggtt gtcgccgaca
     6001 ttggccaatc catcgggatt ggtaaagcgg ctcaagacca cctgcgccag ggcgcgagtc
     6061 tgcccgttcg taaactgtgc catgaccttg ccttccttat cgacgctgat gcgatccagc
     6121 gtgcctgagg aatagccgtt ttgagtctgg ctttgtacgc cggacgctgc accgaattgc
     6181 gtgagggagg cgagattcac cgagatgctt tgtggcgtcg tggcgccatt cgtcagcgcg
     6241 gcggtgatgg tttgtgttcc gcctgcggtc acagctccgc tggcattaaa ggtcatattg
     6301 gtttgcgcgg tggccgctcc gccatcgatt tgggaataga ccccccaggc attcgcggca
     6361 gttttccgga aatacaactg catctggtgc gaattgccca gcgagtcata aaatgtcatc
     6421 cctgtggaga agttgtatga ggtagtgtca gtcgtcgcga aggccgccgt gggtgccgtg
     6481 gcgttggcat tgaggttccc gagaatggtt gcagtactgg tggattgtgg cgggaccgca
     6541 ctcgaggtca atgtgatccc tccgatcgta ctcgtgatgt ttcccagcgt atcggcctga
     6601 taaccttgca gaagcgctcc gcttgcatca accacctgcc ccacgctgtc gaccttgaat
     6661 tgtccggccc gtgtatagaa gttcgatccg gaagcatttc gtaccaggta aaacccgttg
     6721 ccgtcgatgg ccagatcgaa cgtattcccc gtggtggtca tggatccctg ggaaaaattc
     6781 gtttgcacat cgttgaggta cacgccgagg ccgatctgat cgcttcccgc gccgcctgcc
     6841 atgctggaag aaatcaaatc tgaaaaggtc gcccggcttg atttgaatcc cgccgtgccc
     6901 acgttggcaa tgttattgcc gatgacaccc atggcgttcc cgtatgaact taatccggtc
     6961 accgcggtga acatcgaagt cagaatgccc atgatctctc ctccttgttg ccgtactctg
     7021 tcgcgtgcgc tatctgcatt cggccggcat tcacaccggc ctcgatcagg ctactgccgt
     7081 cgtgccggtc tgtgtggtct tgttctgcgc cgactcctgg agcgccacgc ttttctccat
     7141 caaggacacc attttcgtca tctggtccag ttgtgagaat tgcgcggtct gcgcaataaa
     7201 cgcttcattt tccatcggct tcaggggatc ctggtgttgg agctgcgtga cgagcaattt
     7261 gagaaaatcg tccttgccga gcggtttagg tccctcagtc gtcggcgtcg acggatccgc
     7321 tgcaggtgtc tgggtaatca ctttactgat atccatagtt cctccaatca ggcaaaaaag
     7381 ctcacccgtt cgttcgatcc ggcaccacgc tctccccttg ctccgcgctc atcctctcgt
     7441 gccaccgtct gggatagacc tggtgatgat ggacggttct gctgctgttg ctgttgttga
     7501 aacatccagg cgttgtctcc gcgtccttgt tgctggtcga ccgtgacccg caacatgccc
     7561 atttcgaggc ctgacgtgcg caacgacgac tccaacgatt gtccctgttg cagcagcccc
     7621 tgacccaact ctccatgttc cgttcggata tgcgcatgaa ccgtctggtc gctcatcatg
     7681 acccggacgc gcaacggccc catatctagc ggatccagat ccagtgtcac gctctgtgac
     7741 acgggagcca tccctcgaag ctcaccgagc cgttccgatt gagaagcacg gagcactgcg
     7801 gtggtctggc cggattcgct tcgaccggac gaactgtcgt tgacggaagg cgtcgattgg
     7861 tgaacgtcat tgatcctatc catgaatgcc ggccgggcat tctgctcgac tgacggcacc
     7921 tggttgggaa gggaccgatc agttccgccg gatgaatcgg aaagattctg tccgtccggc
     7981 tccagaaatc ggcctgtgct ggatcccgct tgcccctcgc tcagtgacgc accttgcgcc
     8041 agtgcatcaa gaatcgccga acggtccgcg ccctcactcg ggccttgttc cgccatgtgc
     8101 gggttttgct tcgacgaggt catggcagca atggcgggat tcatgcgatc cgcatctgtc
     8161 cgagccgctg cagtcgagtc tgtgcggtct tccactctgg cgtccggcga agaattgtcg
     8221 ccggcgaggg atggcagctc tttcgctgtt gtctgcacca tctcgttccc gttctctgcc
     8281 cgttccttag cctgggccgc gtccttgcct gcgtctgctt taggatccga ctgacccgca
     8341 agaacctctt gcgtaccagc accagcttca gatgtcgccg cgaccagagt cgtgactgag
     8401 gcagacccat cttcggcgcg atcgcttgaa atggctgcag ccgcgttgac ggacgtctca
     8461 gagtggctgt ccgactgagc ctcagcttga ggtgtctgct gttcaggtgg agcggcctga
     8521 gcagtgggca ccatcatgga ggcgagcaac atgtcctgcg acacggcgtc ttgcccggga
     8581 tctttctttt tggattcctt ggtggtgctc ttttcttctg atttgtctgt ggacggctgt
     8641 gccttctccc cggcacattc acaccggttc gacgagtgat catcggacgg tgctacgctt
     8701 gatcgctccg atggttggct ggaggagtcg gagatagagc gactgacttg gcgcgcgtgg
     8761 agacctgcct tctccccgct gcgaggcgta ggagcctcgt gtttgggtgg cgtctgtcgc
     8821 gcctctgccc gcccgagcac cgactcgaag ttccgtgatg gatccgaacc gtgaaccgcc
     8881 ggctgacgat ccctggcatc ctgaatcccc tgtgacgagc ggcttggaaa tccgttgaag
     8941 actggttgca tatcagtggc catacaattc ctgtctgtga cgagaccgtg aatagacagt
     9001 ggagactatt acaaaggcca tgccatgaga ctgccgcagg aaaatggcct ctgacgcgag
     9061 gttttgtgat ccgagaggga atcgagaatg gagagaagga aatttctgca gttgaagatc
     9121 ggcaaaagct gccgtgagaa cgagacgtga aacgaatgac tagggtgtgg tcagcaattg
     9181 ttcggtcaac cgggctgctc gctccggctt gacctccgcg agaatggcgc ccgcggattt
     9241 gcctttgagc agccgaagca cttccagcgc tttacgttca ggcatacgtt ccagtctggc
     9301 tgcggcttct tcggccggca tggcttcata gatcttcgca agttgggcct gattcacgtt
     9361 gacagccgga gtggctttgg ccgtcgtctt agccttttcg gcttcgcccg cgcctttggc
     9421 ctgagcagcc tggcggcgct tcttctccgc ctccaccgct tgctcatggc gggtcacgat
     9481 ttgttccaac tccgccttga gctccagaag atgcgtctcg gatccacgca cggccgtttc
     9541 gcgtttgtct aagagccgtt tccgttgttc caacatttca tagatttcac gtggggcgct
     9601 ggtcgccggg ccttgcacat cttttttcac gctctcaggc ttggtctctt ctttctgcgg
     9661 cacagcagcc ggtttcactt cttctgtcgc atgggcggtc gtgatgagcg tgaggaacac
     9721 cgcaccaaat gctatcagcg ccaccacgcc aacacacctg ccctgctcag agcggctccc
     9781 cgtctccatc atgcccgttc tcccttatgc aatgggcttc gtctccaggc acgttcatcg
     9841 agtaattgct gatcctggcg ctcacgacgg cgtcgtatct ccagcgcggc tcgcgcatcg
     9901 aggagatcga gctttttgcg atactgcatc gcttcgagaa gttctgcgcg cttctgttcc
     9961 atcctcgctt gctcggtcgc caatgcctgt tccaaccctt tccgtgctgc gatggcctga
    10021 tccatggcgt cgagatggcc gtagacctga tccacggtgc tgcctgcacg aacctgatgc
    10081 agataccggt cggcatcttc tttggctcgg gattccaaca tggccattcg attcaccgtt
    10141 tgttgcaggg catgactgag ttccgccaat tccagtttgg cgacttcctc cagttgcacc
    10201 gcataggctc ggagcacagt cacgttcatt gaatcgtccc tgccagtgcc agaagtgcgt
    10261 cgatggattg cggcaacccg accgcttgtt tggtctcctg ccgcagaaac gccgtaatgc
    10321 cgtcgtgggc cgagacggcc ttatcgagat ctttgcttgt accgggcttg tatgcgccaa
    10381 gggtaatcag atcttccgag cgacgataca gtgccgtacg ttcgagtacc aggcgcgcgg
    10441 ccgcataatg ggcgggcgtc acgatatccc gcatcacccg gctggtgctc tgcagaatat
    10501 caattgccgg aaaatgatta cgcgcggcga gttcacgcga cagcacgata tgtccgtcaa
    10561 gaatcgagcg cacggcgtcc gcgaccggat cattgagatc gtcgccgtcg accagcacgg
    10621 tgtagagccc ggtaatgctc cccccgcttt gtgccggccc gacccgctcc agtagcttcg
    10681 gcaggacggc aaatacagag ggggtatagc ccttggtcgt cggcggctca ccaatggcca
    10741 ggccgacttc tcgctggctg taggccaatc gtgaaagcga gtccatcatg agcaggacat
    10801 gtttgccgca gtcgcggaaa tattccgcaa tggcagtggc gaccagggct ccacgaatgc
    10861 ggacgagtgg tggttggtcg gatgtcgcca cgacgacgat ggaccgagcc cgtgcttcag
    10921 gcgtgagatc gcgttccagg aattctttga cttcccgccc ccgctccccg accagggcaa
    10981 tcacccgcac atccgcctgc gtatagcggc tgatcatgcc caggagcacg cttttcccca
    11041 cgccggatcc ggcgaacaca ccgatcttct gaccttgccc gcatgtcagc aaggcattga
    11101 tggcccgaat gccaagatca acgggttgcg tgattctggc tcgatgcaaa ggattcaatg
    11161 gcggcgcata caacggcaca cgcacgtcgc tgcggagggc cggcaagcca tccaagggat
    11221 ggccgaggcc gtcaaaaata cgtcctaaca tctgtggccc gacggggacg gaggcgacat
    11281 gtcccttcat caccactcgg ctgcccggcc cgatcccctg catttcaccc aatggcatca
    11341 gcaacacgcg atcttcgcgg aacccgacga cttccgccat cacggacccg tggcccccct
    11401 cacgtgtgat ctcacagact tctcccacgg aggtgaacgg tcctgaggct tcaatgacaa
    11461 tgcccacagc ctgagcgacc ctgcccgtga ccgataaggg ttcgacatgt tcgaggcgtg
    11521 cgctaagctt caccggcatc ccgctttctt aaggcttcgc cgagtcgcaa taattgggtg
    11581 tccagtgtcg catcgatcag caggctctgc gcctggacga ggcaccctcc tggactgatg
    11641 gaagggtcgg tctcaaattt taatgtcaac agtccatggg gtgtctgact caatgccgtt
    11701 cgtgccgatt cgagcacggt gagatcggac ggatgcaccc gtacacgtac gagcccgctt
    11761 tcatgcaggt gtgcgagagc ggtgcggact tgcgtcacga tcaggtcgcg cttttccgtc
    11821 acgacatccc gcaccacctt gtgggcgatc ataaacgaga gggacgcgat ttcttcttcg
    11881 accgtctcgt gcaggctttg ccaggtttct tccaagcgtg tcatcaggga tgccacgagg
    11941 ctctgctcac gctgccgcgc cgcagccacc cgctcggtcg aacgcttttc cccttccgca
    12001 acaccccgtt caaactcggt aagatcttca gttccaaacg gccgattgcc gcggctgaaa
    12061 tccgcaagcg gcacgggacg gagttgaata ttgccggagc gaatcaacgt gcgggtgagc
    12121 acggtatttt cctgtcgcaa ccggtagagt tccagcaatc tggacaccgc cagccggacc
    12181 agctcgactt ggaacggttt ggtaagaaaa tccgccgctc cggatttcat ggctctcacg
    12241 gccagttcga tgttgccgtg gcctgtcatc acgatcccga gcaagcgatt atcgatgtcg
    12301 agcgcctgct gcagaaaggc gatgccatcg gcgccgccaa gctgcacgtc cacaatcacg
    12361 acagcaacgg agcgggttcg cagatgactc aacgcttccg gaacggagcc ggcggtgaga
    12421 acgggatgcc ccgtggactc gagcatctcg gccagcagca agcagatgga gggctcgtca
    12481 tcgacgatca ggacggcccg cggcgacagt tccttttctg actcgggcga agccgccatc
    12541 gaaacgcccg tgcccgcggc agcgctcccc atttacaata actcctcgcc tccgccgcct
    12601 ccgatgacga tgcggccttc ctcctcgagt ttgcggcaga ctttgagaat attctgctgg
    12661 gacttttcga cgtccgacac cttgaccggc ccgcgtgtct ccatatcctc tttcagcatt
    12721 tctgccgcac ggctcgacat gttcttcagg aacttgtctt tgatttcagg cgtcgcgccc
    12781 cgcaacgcga tgggcaggtc ttccttgctg atttctttca tgagctcttg catgcctcga
    12841 gaatccagat cgaccaggtc atcgaagaca aacatcaacg cacggatgct ttccgccagg
    12901 gcctgatcac gttcggtgat cttggccatg atggcgcctt cggtgttctt atcgacgcgg
    12961 gtcaggatgt cagccatcag ctccgcgcct ccgacggaca tgctcgacat ccccatcgac
    13021 gccgacagga tttcctgcaa actgtcgctg agctcggcca gcacttcagg ctgcacttcc
    13081 tgcatggtgg ccagccgaag agagacgtcg gcatgcagat gcacaggcaa cagagccaga
    13141 accgcgctcg cctgctccgc ctccatttgc cccaggcaga cagcaatggt ctgcggatgt
    13201 tcgatcttga gaatctgcgc gaccgtgcga gcgtcgaccc atttcaatgc gtcgatcccc
    13261 ggataggttt tggtgttcag ggattccatc atccgcgcag ccttttccgg gcccagcgcc
    13321 tttttcagga tggcttccat gaattcccgt ccttcgaatt gcacctcgcc tgaagagctc
    13381 gcctgctcaa attccgcgat cacgctgtct tcttcctgct tcgtgatatt cgcggtttct
    13441 ttcatgaact cgccgagctt ccgaatatct ttcggatcga gcgacttcat gaccgccgcg
    13501 gcggcctcct ccccgatcgc tcgaagtaaa atggcagctt tttgcgcgcc gctgagctct
    13561 ttgttgatgg ccatacgcgt tacgcgttac tcttcagcca ctgcttgacc acgacggcgg
    13621 tgttcgcagg gttgtttttc gccatgtcga ggatctggcc aggctgcttg ccgctgatgg
    13681 cggcctcaac ctgtccgacc gatgcgggca gtgccggcgt ctcgccgact gcctcgaccg
    13741 gagcggactg tacgagcatg accattaacg gccgaacgac catgaagaga atcaggaaga
    13801 acagcagtcc gcccacggca taccggatat acggcatcca cgccctggtg ctgtcagccg
    13861 cagcttcgac accagcgccg accggttctt ccactccaag cccgaactgg atactcacca
    13921 cctccacctg atcttgacgt tcggtggaat agcccatggc cttcttgacg atttcttcga
    13981 tgcgcttcat ttcttcttcc gaacgaggca catacttctt cggctgctcg gcagcaccct
    14041 ccgctgcttt gccaccgccc tcatacgtcc cgtcaaccag caccgcgacc gagagctttt
    14101 tgatcgtgcc ggtcggctcg acgatgcggg atacggtccg gctgatctcg tagttcaccg
    14161 tctcattctt cgtctggtta ttgttcgagc tggtttgtcc cccctcgcct tctgccccac
    14221 cgggcacatt cgattccacg ccgggtacac cgccggtggt cccattgacg ccgctcgact
    14281 tttcctgtcc gcgctgttcg ctgcgtacga cttggccgtt cgggtcatag cgttcttccg
    14341 tggtctcgat tttccggaaa tcgaggacac tcgacacccg gacgacggcc ttattcactc
    14401 caacaatgcg ttccagcatg gtttgaatgc gcgtctcgat gttcttctcc aaggtgcgct
    14461 ggtattccat ttgagtgccg gacagaccgg ccgattcatc gctactggaa ttggagagga
    14521 gatttccgtg accatcgacc acggtcacgt cgcgagcctg caatccttcc acactgctgg
    14581 agacgagatg cacgatgccc tgaatctgag ctttcgataa gttctggttg ggccggagag
    14641 atacgacgac cgatgcgcgt gcgcgatcct gttcagttgc aaacagccgc cgctcaggaa
    14701 tggctaagtg aacacgagcc cgttcgactt ccggcatctg cgtgatcgta cgcgccaatt
    14761 ctccttgaag cgctcgccgg tagttcagct tctgcacaaa atccgacatg cccatagtgg
    14821 tgcgatcaaa aatttcgtaa cccacaccgc cgccatgcgg gagcccctgc ccggccattt
    14881 ccagccgtag atcgtgcacc tgcgcattgg gcacgaggat ggtggtgccg ccgttggtgg
    14941 tctcataggg cactttggcg tcttttaatt tgtcgatgat acccgcggca tcgtcggtcg
    15001 ccagattggc aaacagaacc tgcatgtccg gttgctgtgt ccagagcgtc acggccacca
    15061 gccccgcgat ggatccggcc agggcgagca gaatgatgaa ccgttggttg atcgtgaact
    15121 ggctgaattt tgaaaacatg tgctgtgaaa tcctttatgc gtgatatccg cgcgggagtc
    15181 cgatcgctaa atctgcatcc gttggatttc ctcgtaggcc gtcacgagtt tgtttcgcac
    15241 ctgcatcatg agctggaagg aggcatcagc ctgttgcaac gccaccatgg tttgatgaat
    15301 gttggtattt tggccggtca ccaacgactc aacggcctga ctggcccctg cttgcgcatc
    15361 gttgatatgc ccgatcgctt ctttgagcga acccaaaaaa ttggcccctc ctgcctcgcc
    15421 ggtttgccca gcttgctgga gcccgggcgc ttcgatcggc ttgatgagat tggcacccgc
    15481 gatgtgcaga tcggacatga ttacctcccg atttccaggg cgcgattcca catggtacgg
    15541 gtcgcattga cggcttgtac gttagcctca taggctcgag acgcgccgat catattgacc
    15601 atttcttcca tgacgttcac gttcggcatc gtgacgaacc ccttcttgtc ggcatcagga
    15661 tgacgcgggt catacaccgt ttgccccggt ttctgatctt cgatgacccg ggcgactttc
    15721 actccatcca aggcatgtcg gcccgatcct ttggacactt gcgtgaacgc cttctgaaag
    15781 gccgaggaca cgggggctgc ctgaaacacg acatcacgcc gacgatacgg gccaccggta
    15841 ctggttttgg tggattgcgc attggccagg ttgctggcaa tcacattcaa ccggtgtcgt
    15901 tgtgcatcga gcgccgatac ggagacggcg agactatctg ataaatccat cacacacctc
    15961 gttatggttc gcccatgact cgaacaagcg catcactgta tccccgaaag actaccgtcc
    16021 ttcgcgaatc gcactaagga gcgttttgaa tctcatgctg atgatggaag cggacgtgtt
    16081 gtactgctgg gcgttgtccg acatcttggc catttccagt tcgatgttga cggaattggc
    16141 atcgagaggc agatcgccgg caggaacctc ttcaatgcgt ccctgtactc gttgaaaccc
    16201 gctgcccttc ggcccgatgt gctgctgatg ggttgagacc aaggcgatcg gcagtttgcc
    16261 ctgttgcgca ttcgccagag cctgaccgaa gttcaggtcc gtggcgcgat acttcggggt
    16321 ttcctcattg gcaatgttgg ctgcaatcac ctgctggcgg gccccgcgca gatccaatga
    16381 acgttcaagc agctgcatgg tccggtcaaa aatggtcatg gtccacactc cgtagaaagt
    16441 tcggtgttca acggtgcaat ctgtgtaccc cgttcagaat ggggattttt ccgggttcac
    16501 ccgagtctgt gtgacaatgt gcgctcggcc ggcaccgggc aaatacgacc tagagggaaa
    16561 attttgccca gcatcgggcg aatgagacgt cagggttttt ctgcctccac ctgatatccc
    16621 agatcacgat attcccgtaa cttattgcgc aacgtgcgga tactgatgcc caactccttg
    16681 gcggcatgcg tccgattctc tttgactcgc gccaaggtct tgaagatcag atcccgttcc
    16741 atttcccaca gcgacccgtg cgctgagggc gcgacgagcg tttctacggt ttcgactggt
    16801 tggatgggaa ccgttccgtc ccccggcatc aagtgatcga cgtcaaccgc gttccccgtt
    16861 gtcactaaaa tggcgcgttc catgacattt tccagctcgc gcacgttccc cttccatgcc
    16921 cgctgctgaa gatctgcgat ggcgcgctcg gacagggtcg gcacggtaag gccattgcgt
    16981 tgggccgagg aacggagaaa atgtctggcg agcagcggaa tgtcgcccgg tcgttcacgg
    17041 agcggaggca ccgtgacggg aaatacattc aagcggtaaa acaggtcctc tcggaaacgc
    17101 ccttgcgtca cctcgtggta gagagagcga ttcgtcgtcg cgatcacacg gatgttgacc
    17161 gggaccggat ctcgccctcc gatgcgatca acttccctct cctgcaacac tcgcaagagt
    17221 ttcgattgca ggcctaaatt catttcgctg atttcatcga gaaacagcgt gccggtatgg
    17281 gccatttcaa acttgcccag ctttttctga atcgccccgg taaacgcccc gcgctcatgc
    17341 ccgaacaact cactctccag caaactgtcc ggaagcgccg cgcagttgag ggccacgaaa
    17401 ggtcgatggg cacgggggct tctcgcatga atgtaccggg ccagtaactc ttttcccgtg
    17461 ccgctttcgc cgctgatcaa cacggtggct tggctggcgg cgaccccttc cagcgtcgtg
    17521 agcagacgaa tcatgccggg atcttgcgtg agtatcgcgc gtgcctccag cgccgcaggg
    17581 gcatcctgct catcaggctc tgcgctggct tgaagattga gaatcacccg ctcgagcagg
    17641 tcggtcgaga acggttttaa aatataatca ttcgcgccat atttcatggc ttcgacagcc
    17701 gtctcgacgg tgccgtacgc ggtcataaga atgatgaacg tctgcggagc tttctgtttt
    17761 agggctttga cgagctcgag cccgttcaga cggggcattt ttaagtcggt caccaccaac
    17821 cagggcttca accgttccac ctgctcgatc gcatccgccc cgtcgatggc gctacggact
    17881 tgatacccca ttcgacgcac ggtttctgac aatgccgttc gcatcgacgg ttcatcatcc
    17941 acgatcaaga ccatgcgagc ctcatcggca gaagcggaag gtccttgtaa ggctggttgc
    18001 gcgctcattc gtacctcttt tcttcggtga tgctcgtaat gtgacgtgaa cgttctgaag
    18061 gggcagacac ggtgacaagt gaaacaggcg tcgacgaagg ttgtcgtggt cctcgcggga
    18121 gggtgatcac aaacgaggtg ccatggcccg ggcgactttc cacatcaatc cgcccgtggt
    18181 gggcttcgac cagggcgtgc acgatggcga ggcccaaccc ggtcccctga tccttcgtgg
    18241 taaagaacgg atcaaatact ctcgactgca gttcagccgg aattcccgta ccggtgtcgc
    18301 ttaccgtgat atgaaccgcc ggtacctcct ggatctccgg aggggatgga caggccgtga
    18361 gcgtgagcgt cccgcccttc ggcatggcct gaaccgcatt caagactaaa tttaagagga
    18421 cctgtttcat cttggggccg tcgcaccaga gctggggtac aagcggatgc atctggcagc
    18481 ggacgtcgac ggtgtccggc gggatggcgt gactggccag tgtcagcact tcacgtatca
    18541 gtacttccgt gtcatgccat cccaggcgcg agcagtcggg acgggtatac acgagcaggt
    18601 tcgagaggag ctgatccatg gattgaaccg ccatggaaat atgttccgca taactccgga
    18661 gttgaggctg gtcccgaagg tccttgcgta acatggaggc aaataactcg acactgccta
    18721 acgggtttcg aatttcgtgg gcgatgctgc cgaccatctg gcccatggcc tccaaccggt
    18781 tgcgccgttg caaccgatcc tcaagctggc gaatgcgcgt cacgtcatgg atcaacacca
    18841 gttttccgat agggttcccg gattcgtcgg tgaggtcggt ttgtgagagg gcgatggtga
    18901 cgccgctggg gagcacgagc gggtactctc catggtcttt gcggatttct gccaacaggg
    18961 cgcttgcctg gcgccctcgc agttgagcct gtggtacgcc cagcagctgc tctgtggaat
    19021 gattgcaccg caccaccaca tcctgtgagt cggccacgat gacgccggtg tccagggatt
    19081 ccaacacggc cgtgacatgg gcacgcattt cctcggtttc gcgaagatgc tcgcggagac
    19141 tggcattggt ttgcgcgagc tcgagatcca tttgctggag ccgcgtcgtc aacgcatcgt
    19201 acgactgctg caggacggtg gcggcctgat caaaatcgcg aaacgcgcgc gtcagcaaat
    19261 cgttgttggt ggtgtcgcct tcctgcctga tcacggtttt ttcccccgtt tatcgaccgg
    19321 tggttccggc aattcattgt ccaacatggc tgccatgcgg cgaacgagac tatcaggatt
    19381 gacgctcagt gcacgcagac cgtttttcgc gaaatcctcg cgcttctcac cgcgtgccag
    19441 ttgaaccatc tgtagttggg cccacgcctc ctgctccagc tcaaggccag cgacgacagc
    19501 ctctttgtac atggccagtg cctgcgaccg gcgattcaga cgagccagca cctcggcatg
    19561 ccgcagcagg tctgccgcgg gaagttcctg accggctttg atcaggccgt cgtagatagc
    19621 cgccgcctcg gcgggttgtt tggactgcgc caacacggcc gcgagtttga gctgcaccat
    19681 cgtgcggtca tggtgccgcg gattgtgagc caaccattgt ttgcccagct tgagcagggc
    19741 ggcgagattt ccttccccct cgaacgagtt cagaattcca cgcaaggcct ccccggaaaa
    19801 ccggccggtt ggaaattcca ggcggtaccg ttcaaagacc gagcgcgcgg cccgcatatc
    19861 tttttgttcc atgtagctgt aagccagccc catgagcgcc gcttcatgga acttctctgc
    19921 ctttgcatcg cgaaccagtg actggaacag ccgcgcggac tccacgggaa atcctaagcg
    19981 acgatgtgcc tctgcaatct tgagcaacag ctctgtgccg gcatacaacc ggtcggcaaa
    20041 cggcccgtgc cgatggaaga tgttgatcag ttcaaaatcg tcttctgcct taagcgcagc
    20101 ttcaagacga gggcgaagga aacggaccag ctgcacgccg cctttttccg gccagggatc
    20161 gttgtcgaat tttccactgc gtaacaccac ctgttcatac gcacggacgg caccttctgg
    20221 cttgcctgcc ctctccaagg tttctccgag gtggaatagc gcctcgctgc cgacaattga
    20281 tccttcattg tccttggcac tctgctcgaa taatcggcga ggactgagca tctcccctgc
    20341 cttcaaggga acatggtcca gatgcgccga aatggtatgt cgaagattga tctcttctcc
    20401 ctccggatcc agatgttcct gcacatcggc atatcgaagg cgggctgtcg cggccactgg
    20461 agcctcgggg tattggctta ataacgctgc gtagaaaatg ctcgactcag cccaccgccc
    20521 ggcttctttg taactatcgg ccaggtaagt cagcacaaac ggagtttcag gccggtttgg
    20581 atatagattg tagaaatgca gcaactgctc ccgcatgacc ggaccgcgct tggcctcccc
    20641 tgccgtatca gcataccgca gcaacgcaaa ggcatcgctc cgcagcgcgg ccggccatcg
    20701 gccggaaata ctctcgtaga gcgcgtcggc atctttcatc cggcccagcc gatagagact
    20761 gtgggcttcg cccaaggagg cagagaccag cagcgtggga tccacggtcc gcttcaatac
    20821 gtgatcgaag gtttgggtgc tctctttcca atttttaaaa cctcgctgga cataggcgag
    20881 accgagcatg gcccggttgg catcaaacga gtcgatttcc gacagggaaa gcgcatgctg
    20941 ataggcaatc tgcgcttcct ggaaccaccc ttcgatgcga tagacatcgc cgatgcgcca
    21001 ggcggcccgc ttggcattcg tcgattgcgg gttctcgcgc atgagtgttt tgtactgatc
    21061 gatgatgtcc agtgggcgga caccagggtc gtgactctga agcgtacttt ccgcgaggaa
    21121 cgcctggatg gacgatctga gcggcgactc ccggcggtca cgaagcatgt tcgtgaaatg
    21181 gaggcgtgcc tcgagatatt gcccttgatg gtagagaccg actccctgtt cgaactgctt
    21241 ccattccgga atttccggca gccgctcgaa acgtggacct ggatcaggta atgctccacg
    21301 aacgagctcc gatggctccg ccatcatggc tggcagctct tgtcgcatcg tggaaaggcc
    21361 acccgtggcc gtggcgggga cgattcctac tagtagaagc caacttacaa taggagtgag
    21421 gacgtagcgg gctgtggtgg atgaatttcg cacaggcggc aattcagcaa gtcctgtgcc
    21481 cctagaggag tccggtaaca cactgcatcg cttgatgatt taggcgaacc gaggcggaaa
    21541 actgagagag gtgtcagaaa taccaacggc gggaacgagg aagcggtcaa aaaaatgaca
    21601 tggcaagaga ggtcgtaaga tttgtcagtt ttggatcgag tagccgtgac ttttcggatc
    21661 aaagcctttc cgtttcagtt tttccaccag tgttgtccga ttgacctgca gcaactgcgc
    21721 ggcccgactg gttatgccat ttgccttgcg tagcgcttcc ccaatgagac ggttttcgta
    21781 atgttcgagt tctttggtca gattgatacc atcttcggag aagcggataa attgctcggg
    21841 ggcctcgacc gcctttccgc ctgccgtttt caacgcacgt tcaggaagat cggccagcgt
    21901 aatcattccc ctcttcttca acacgcagag acgttcgatc atgttctcaa gttcacggat
    21961 atttcccggc cattcctctt cggtcatgcg aaccagggca tccggctcca tgccaagaat
    22021 ctcggtcctg cgtagttgat tgaattgagc gataaagtgg ttcacgagga gcggaatatc
    22081 gctgcgccgt tcccgtaatg gaggaatgtg aatgggaatg acgtgcaatc ggtaatacaa
    22141 gtcctgccgg aatcgtcgct cctgtacggc ttgcgccaaa tcctgattgg tggcggcaat
    22201 aatccgcaca tccacgttta tagtcctcgt tccgccaact cgttcgaagc atcgttcctg
    22261 taatacccgt aacagtttca cctgcaacgg caggctcatt tcaccgactt catctaaaaa
    22321 gatggtgccg ccatgggcca attcaaaacg gcccagccgc gtgtgcgccg ctccggtaaa
    22381 ggctcccttt tcatggccga acagctccga ttccagtagc gtttcaggaa tcgccccgca
    22441 attaacgggc accagtggac ggtctcggcg catgctattg aaatgcagca ttcgcgcgat
    22501 caattccttt cccgttccgc tctccccctc gatcagtacg gtgctatcgc tatcggcaac
    22561 cttctcgaca aaatccagca ccatccgcat gggcgcgctg gtacccacta aatgttcaag
    22621 acgatactgg tctcgtaccg cttttcgtag cagatgattt tcctgtttaa gtttgtagac
    22681 gtccaatgct tttcgcacca cgaccgcaac ggtgtcaggc tcaaacggtt tggtgataaa
    22741 gtcgaacgcg cccgccttca tcgctcgcac ggcataatca accgtgccat agccggtcat
    22801 cacgatcgca atcactttgg agtcgatttt tgagattcgg tctatggttt ccagtccgtc
    22861 aatccccggc atctgaagat ccgtcagtac gacgtgtacc ggctgatcct tcaccgcctc
    22921 cactccatcc agtccgctcg acatgaccgt gacctgatgg ccttcgctcg taagtgtttc
    22981 cgccagtagt tggcggactg cgggatcatc atcgatcacg agaatgtgtg actggctcat
    23041 aagggagcac tcctctctgg aagaccgcaa tctcggagat ggctggatgc aagatgactc
    23101 tggacaggaa gaaactgagc gattcgcatg gtagacatat gttggttcag cttattggga
    23161 attcagactt tattcaagta aaagtaaggc cttgcgggaa aattgcgctc g
//
LOCUS       DAOQEW010000043        23060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_540_length_23060_cov_8.445816, whole genome shotgun sequence.
ACCESSION   DAOQEW010000043 DAOQEW010000000
VERSION     DAOQEW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 23060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23060
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_540_length_23060_cov_8.445816"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PLS35_06835"
     CDS             <1..1553
                     /locus_tag="PLS35_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNI18408.1"
                     /translation="FHEFVIYQLHVGTFFTPNLPHKGGTFLDVARKLPYLADLGVTAL
                     QLMPIQEFQTSFSLGYNGTDYFSPEMDFAVADAELPDYVAEVNKLLDAKGLRRYHAAD
                     LHGEMNQLKALVDLAHLHGLAVLLDVVYNHAGGEFGDQSLYFFDRQDPAGGQWNSLYF
                     TDRGHAGGLVFDFSKPEVRDFLIQNAKFFLGEYRIDGFRYDQVSVIDHDGAPDGWRFC
                     QDLTSTVHAQRPKTLHHAEYWDVNPFIIAPPPVGAGFDTTLTDGLRIAIRDVIANASA
                     PDERPLHMTQLAQSLWPQGFSESWRFVQGPENHDIVYNGREARIARLGDPDHPRSWFA
                     RSRARVATGLSLTAPGIPMLFMGQEFLEDKQWSDDFVSHRDLLLYWAGLDQGDRQMLD
                     HLRFTRELLALRRLLPALRGRGFRATHMHDQNRVLAFHRWVEGEGQDVMVILHLANFT
                     RVGYQIGLPGEGAWREVFNSDVYENWVNAGVAGNNGRVLANPQPLHGFGYSAGLVLPA
                     NSILIFAR"
     gene            1789..2112
                     /locus_tag="PLS35_06840"
     CDS             1789..2112
                     /locus_tag="PLS35_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18409.1"
                     /translation="MMTRKILATLMFVWGIGLVSLAQAFQTAVVFPVSSVEHVDSRNQ
                     VLTFKTQDGQVRMFRVAQSVGMKRESLAKGDLVRIEVDLDDQIVNIVKLTQTSLSDRE
                     ALRRK"
     gene            complement(2166..2981)
                     /locus_tag="PLS35_06845"
     CDS             complement(2166..2981)
                     /locus_tag="PLS35_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18410.1"
                     /translation="MATTPDISRAWPPDDHYSLLQRCRSLYHELWFRLSERIRWSRQP
                     YHEKPIGCLAGLTSWQIARINELQVRYDLRFETHYGQHTALANYAYLDLLHQAWTDTN
                     RPVPQGGVVADVGCANFWYARTLHAFFRPATLTGVDVEGFRLYPTGYSRYDAARGYIA
                     GLSQTSFLVANYCEVETKADVITAWFPFVTPSPVLAWRLPLTVFSPERLFDRIARNLT
                     SQGLFFMVNQGSDEAAIAADYGRRAGLRLVDRWVHPRPLRTRPHPPVVTWWTT"
     gene            complement(3072..3527)
                     /locus_tag="PLS35_06850"
     CDS             complement(3072..3527)
                     /locus_tag="PLS35_06850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI18411.1"
                     /translation="MTQAGEPIRVELVDWVHAEQLIRPIRETVFIREQAVPEELEWDG
                     LDPQCVHVLAWNKQGDAIGTARMQAQGTIGRMAVLKDWRGRGAGRALLEALLECAVRQ
                     GLTRVTLSAQTHAIGFYERAGFHVIGELFMDAGIPHRRMVKELIRQESE"
     gene            3755..4015
                     /locus_tag="PLS35_06855"
     CDS             3755..4015
                     /locus_tag="PLS35_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18412.1"
                     /translation="MNIQQRRARVGSLRKTRAGMWRAWCHAGAKEARPSVFSWDFLRS
                     VWSLLCRMTRNKGVRPDENRFAWQEHIGCTAPRSSGIYGRMI"
     gene            4293..5147
                     /locus_tag="PLS35_06860"
     CDS             4293..5147
                     /locus_tag="PLS35_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI18413.1"
                     /translation="MKVLIATDGSKYGKWATEWVARMPFAEKPEVALLHVTDVEALRA
                     PFMFQPVVIGNEPFIQEEIKRIEARGKTAITEAKAQMASLKLKGKVQSERGPAGPTIL
                     DRAPRRDGIVAIGSRGLDALDRFMLGSVSTQVTLHAPCSVLIVKEEPRPVSRILFAAD
                     GSKASEKALRFLLTKLQPEAREGFEPIDVVVMHAMPFLKYPELKEAGARLVEQCANKL
                     IKAGYVVDEVVQLGKPADEILKVASKKKVDLIVTGAKGMGAVARFLLGSVSTKVVQHS
                     TCSVLVVR"
     gene            5293..6570
                     /locus_tag="PLS35_06865"
     CDS             5293..6570
                     /locus_tag="PLS35_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18414.1"
                     /translation="MWLYTAGTCRTDNGHMTDRVAALTCEFRRLLPRPACSLHLAGRA
                     LLVVLLMGQVGCDRDPGVPPAAKPVSRAADGIVQLTAAEIGRAGIQVQRAAKEPFVLH
                     REFPATVQANENELAEVTTLIRGRVVDVLVDAGKDVKKGERLALLDSTDLGMAEGVYL
                     KATARQHEAQLAHERAANLHEHRAISLAELQRREAEMKTAQAEAREAGHRLRLLGVPD
                     QEIQRLERERTIRSDIAIRAPFAGRVIMRNLTRGEVVETSRNCFTIADLSDVWVIASV
                     PEKDVQFIHPNQTVHVIVAAYPHSLFSGRMTYISDVLDPTTRTMRIRVTVPNPDRALK
                     PEMFAMVRVDASPQPAVLTVPLSAIQQEGGGKMLFVRQGENRFAPRRVQLGDEQDGQV
                     IVLEGLREGEDVVVKGAFAIKSELDIHKIEPTQ"
     gene            6567..9695
                     /locus_tag="PLS35_06870"
     CDS             6567..9695
                     /locus_tag="PLS35_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNI18415.1"
                     /translation="MIAALLEFSLRQRILILGLACFCAAAGVFAFQSIPIDAYPDVTN
                     VQVQVLTEAAGLSPVEVERFITYPIELQMNGLPGLTEIRSISKFALSQLTVVFEDDVD
                     VYFARQLVLERIMAVRERLPAGMEPVLAPVTTGLGEIYQYYLEGGTKPDTNAALVEEA
                     LTNQRTIQDWVLRPLLKGVPGVIDVNSLGGFVKQYQVLVDPDRLRKYSLTLHDVFDAV
                     ERNNANAGGNVLERHAERAIVRGLGLIKSLADIERIVVKEAGGVPVFVRDVAEVRIGH
                     AVRHGAAVLNGEREVVAGTVLMLRGGNAREVVQAVKQRAESIERQGILPGGLTLVPFY
                     DRIELVTAAIHTVRDALIEGVVLVALVFFLFLGHVRSAIVVTVSLLVTPLITFVVMQR
                     VGLSANLMTLGGLAIGIGEIADGSLVVVENIYRHLSENRLQERSRLEVVLRATNEVGR
                     PILFGILIISVVFLPLMTLHGMEGKMFAPLAYTLVISLLASVIVTLTLSPVLASLILH
                     GDHPEETRLTRWMKAQYQPVLRWTLDHRAAVLLSSTAIVLASLSLLPSVGREFIPILE
                     EGALTPQIVRLPSVSLDESIAIEKQTQKVMLEFPEVRMSVSKIGRPDIAVGPEEPNES
                     DPIVALRPREAWITAQTQAKLVDAIRTRLAEIPGISVLMSQPIQERVDELISGIRTEC
                     AIKLFGDDLDLLYQHAERIADLMRTIDGVKDVKVEQVAGQPYLTIDINREKIARYGIN
                     VSDVQDIISTAVGGRPATQVYEGERRFQLVLRFPESSRNSIGAIGDIRVRSASGAPIP
                     LSELAAIEMREGPARISREQAKRRIYIGFNVVGRDIGGVVDEGRKRLAEQLRLPQGYT
                     VAWGGAFENMERANARLLVVVPITLGLVFFLLFWAFHSLRYAGLIILNLPFALIGGVV
                     SLWLSDQYLSVPASIGFIELFGLAVGNGIVLVSYINQLRNEGQQTEAAIISGCVLRLR
                     PVVMTMMTTLLGLLPLVLAQGIGAEVQRPLATVVIGGLFTSTALTLLVLPALYRTFAE
                     QDVTKEHAPEWV"
     gene            9716..12241
                     /gene="mgtA"
                     /locus_tag="PLS35_06875"
     CDS             9716..12241
                     /gene="mgtA"
                     /locus_tag="PLS35_06875"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNI18416.1"
                     /translation="MLKQTPFWSLSADDVLRQLSATPNGLATAEVDARQATSASVRLK
                     PQRNSRPLRLLMAQFRSPIILILLFASGVSFFLAEHSDALIILTIILVSALLSFWQEY
                     SAARAVAGLLALVQITARAWRDGELRDIPADHIVPGDVIELSAGSSLPGDALVLEAKD
                     LFVDEATLTGETYPVEKSVGSLPAHAPLAKRTNSLFLGTHVVSGQARVVVVAVGTDTE
                     FGRIAHRMALRAPETEFERGVRRFGYLLLEVTLLLVLAIFAVNVYLERPALESFLFSM
                     ALAVGLTPQLLPAIISVNLSHGAKRMAQQNVVVKRLASIENFGSMNVLCSDKTGTLTE
                     GSMRLHAALDLDGQPSERVLFHAHMNAMFETGFPNPLDEALRQHRSFDLGSYRKLEEE
                     PYDFVRKRLSVLVATPQTHLLITKGAVESMLSVCLHAEQQNGSLMPMDQVRASIREQV
                     RGLSSEGYRTIGVAYRDLGAIDRVSKEHETGMTFLGLLVFVDPPKAGIVDTIALLRKL
                     GVSLKMVTGDQGLVAAHVGRQVGLANPRLLTGSDLRGMTDDALRARANEVDIFAEIEP
                     NQKERIIRALRGSGNVVGYLGDGINDAPALHAADVGLSVDGAVDVAKEAADLVLLEHD
                     LSVLVEGVREGRRTFANTLKYVFMATSANFGNMFSMAGASLFLPFLPLLPKQILLTNV
                     LTDIPEMTIATDHVDSELLDRPRRWDIPFIRRFMLTFGLVSSFFDYLTFGILLFLLHA
                     TTAQFRTGWFVESVLSASLIVLVIRTRRPCVTSRPSAPLLWSTLLIALVTILLPITPM
                     GSFLGFEPLPAVFWVALLGILLAYVAAAELAKTFFYRQVNNGR"
     gene            12391..13908
                     /locus_tag="PLS35_06880"
     CDS             12391..13908
                     /locus_tag="PLS35_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18417.1"
                     /translation="MPGSPHSPNGLSYDPQVLLNSQPVIVTVIDPADHSVQFQNQTGL
                     GAFGDMAGSPCYEKIAGKTAPCEFCRMPEALSRDGVVSEQVEMPNGRHLLVHWAKAPT
                     KDGRVHVIETIIDLTKRKQDEQSLRQSQKMEALGRLAAGIAHDFNNLLMVVIGHAHRV
                     AQQLGSHPLHHEVEMIGQAGTRAAALTKKLLTFSRRQVLEQRDVPLNTLIRDMEDILR
                     RLIGEQIQTVIVLHPQAGHVLGDPVQMEQVLLNLALNARDAMAEGGILTIETGNTDVD
                     EQYVRTHPGAVPGPYVTLVVEDTGCGIDAETLAHIFEPFFTTKEFEKGTGLGLATVYG
                     IVKESRGYIDVTSQPGRGTRFTVMLPRVQSQTSEAEQPASSRPLPATCATILLVEDDE
                     GIRRLVAAVLRDQGYEVLAAADGVEALQMLQLRKGGCDLLITDVILPRMKASVLAQGA
                     RTMFPKIKVLYISGYAGDMLVSHGVDAQAAYLQKPFMPQAIIEKVAELIPPAPAQ"
     gene            complement(14112..14480)
                     /locus_tag="PLS35_06885"
     CDS             complement(14112..14480)
                     /locus_tag="PLS35_06885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18418.1"
                     /translation="MSKRKNTRVEMNRPIDVQGPRGTSQGVVKDFSPGGCKIQQTAAK
                     VHCGMRLTLRISLPDRIEPIEIKPAVVTWTSKDAFGVEFLALSAETRSRVKLVYDLLL
                     DAQSTAETERVISLPGVSWK"
     gene            complement(14529..15518)
                     /locus_tag="PLS35_06890"
     CDS             complement(14529..15518)
                     /locus_tag="PLS35_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18419.1"
                     /translation="MPSAMKQLLASALGYALLASGAGLAEEIPHQRSIALIDKTWAVS
                     LDMTGYRVQADGVKPDSRRYVLATNDAAAMQLSVTLEAVSGQATEQGCLAHLHRIAQT
                     AAAPSHAGPTRTEINQLPVFEYLLYETSAGWTDQVHLFACTNKDNVYADIHLSHSGTT
                     TVDASQLRKVLESLSIVAAAQPGSLDYFRAGSAPYLQGQFKQAIPHYERAVALERTNP
                     SLDKALWRLLVHNLGVAYRRSGDLARAKTTIDYGLSQEPANPSFHYDLARTYAGMNER
                     DRAMQSLHDAFLHFRRSNDREPIPDPRQDVSFGRFMLDPAFRTLTESLMQPAI"
     gene            15860..16474
                     /locus_tag="PLS35_06895"
     CDS             15860..16474
                     /locus_tag="PLS35_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18420.1"
                     /translation="MRERDVQAIGTRRGARVGWRTVFGIVAVAMMAQVSSAPAVVHVL
                     PDMTVTRDSGTVTGAKAVRADPTLQEVLAAFDRAERALEKQDLSSLMQFYAPGYNYHG
                     LHVEDVQRIWGEVFEHYRTLSSTHLFSEMKIVRAEDGLRVELTCTGGLYGADVQSGAR
                     ITLDSWFQEVHYLVRDRQGWRFLGNRGDAPRSAPFSSSPHHPLF"
     gene            complement(16493..16945)
                     /locus_tag="PLS35_06900"
     CDS             complement(16493..16945)
                     /locus_tag="PLS35_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18421.1"
                     /translation="MTRSSWSSSNPLPDNLRASLKDLRHGLLGLHKSLIVSEQLTYER
                     IYGRISSAGQLLQLVMNDPWFAWLHPLSHLVVRIDVVLEDHEDTTIETAQDLLLEARH
                     MLRPSEEGDGFERSYYEALQRTPDVVLAHASVKKLLSTAATAASAAAA"
     gene            17238..19958
                     /locus_tag="PLS35_06905"
     CDS             17238..19958
                     /locus_tag="PLS35_06905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNI18422.1"
                     /translation="MVEARQHSQTRWYARSLDDLGREFGVDQALGLDEDEAARRLTAH
                     GPNELPEAAPPSPWHMLGAQFSSLIIWVLIGAALVSGLLGEWVDACAILAIVLLNGLL
                     GFVQEYRAERSLAALKTLAVTYARVIREGVRRTLSSTELVPGDIIDVEAGDHVPADAR
                     LIQATAFLTQEAALTGESTPVEKTSGTLSDGDMPVADQRNMLFMGTTVTGGKGRALIV
                     ATGSGTELGRIATLMTTVPVEPTPLQRRLEQFGHVLLWLSLAIVVVVFGLGLWRGEPL
                     FEMFLTAVSLAVAAIPEGLPAIVTTTLALGVMRMVSRHALIRRLPAVETLGAATVICT
                     DKTGTLTKNEMTVTRLAVDDVVYDVTGEGYAPEGTIVDGDGRTGALRALLWSAVLCNG
                     SSLRAAEGSWTVVGDPTEGALLVAGGKGGWRKEALERAHPLVGEIPFDSERKMMTMIR
                     RSEGRAVAYVKGAPDILLERCDSYMTKSGEVAPLSDSMRRSMVSINQQFAQQALRVVA
                     LAQRVLDPDPATFESKGIEQQLVFLGLAAMKDPLRPEAKQAVERCRSAGITTVMITGD
                     HKETALAIGREAGFASGSTQALSGLELNGLTDAELSSRVRDVCVYARVSAEHKLRIVR
                     AWRAQGAVVAMTGDGVNDAPAVREADIGIAMGMTGTDVTKDASDMVVTDDNFASIAAA
                     VEEGRSIYENIRKAVHYLLSCNLSEVLVMLGSTLLGWPLPLLPIHILWINLVTDGFPA
                     LALAVDPKDPDVMKRPPRDPKAPLLDRQRFVTVCVQGAVMAAATLAVFGIALVIMKDE
                     VTFARTMTFTTLVLVQFLHAFTCRHDRFSLFQIGITTNRMLVAAVLISALLQAGILLS
                     PWGQDIFRVVPLRLDDWWLVAACGMLPFVVMELWKAWSRVRNGAARPSTA"
     gene            complement(19948..20820)
                     /locus_tag="PLS35_06910"
     CDS             complement(19948..20820)
                     /locus_tag="PLS35_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNI18423.1"
                     /translation="MNRSVFALVFTALFLSWTTPGSAHSPLPKPDHVVIVIEENHAFG
                     QIIDSPAAPYLNALARKGALLTNSYAVTHPSQPNYIALFAGTMEGVNGNVCPTPLTAP
                     NLSSTLKQAGQSFIGYSEDLPAVGAVDCIAGAYARKHNPWVNWQASPVNTVPAAQNRP
                     LTDFPTDLSTLPTVSIIVPNQQNDMHDGNDPDRIQRGDHWLQVRLERYVDWAQTHNSL
                     LIITWDEDNGKSDNHIPTILVGPMVRPGRYHEQTNHYGLLRTITDMYGAQPVGLSRQA
                     SPLTTIWAAPKLTP"
     gene            complement(20838..22568)
                     /locus_tag="PLS35_06915"
     CDS             complement(20838..22568)
                     /locus_tag="PLS35_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18424.1"
                     /translation="MFSFKRFYPFLKPYVPRMIAAAVMVMAVAAINLALLRLGGTLWD
                     VITVQHDAPRMTNMILLLLGLVLIQGLCSMGHSYLTAWVSQRVMADFRIHLFAHLETL
                     SVNFFSKRRTGELMSRLMNDVTVIQNVVTDTPIDAAKQIVTFIGGAGFLFAMNWQLCL
                     LILVLLPMLVVVAKLFGRRLRALSTKIQDQTATVSTLVEEVIAGIRVVKSFVQTKREE
                     ERFVTQVQSAMELSLRRATIMAWFVPTIIFVTFAAAALVLWYGGRQVIDGSVSPGDLF
                     AFVLFAGILIGPFGSAARVFAQIKEAQGAMQRVFEILDTHAEIADAPGAIELPAVRGH
                     VRAENLSFAYDPRQPVLSDVSFEAKPGELIAIVGPTGSGKTTIMNLLHRFYDPTSGRL
                     TIDGHDVKNVRLDSLYRQIALVPQDTILFGGPIRDNIRYGREDATEEEIIAASTAAHA
                     HEFIVGFPDGYQTIVGEKGINLSGGQRQRVAIARAILKNPRILLLDEATSALDTESER
                     LVQEALERLMVNRTTFVVAHRLSTIQRADRILVLNKGKIVESGTHAALLEQQGLYHYL
                     YTLRLSELPV"
     gene            complement(22669..22887)
                     /locus_tag="PLS35_06920"
     CDS             complement(22669..22887)
                     /locus_tag="PLS35_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18425.1"
                     /translation="MPEGKKVRIRVRTVNCIYVGDFLIPPMRNRVSDAINEEQRLFIS
                     LTDVLINDKDRSDFVAINKNLIESIAQL"
BASE COUNT         4445 a         6390 c         7367 g         4858 t
ORIGIN      
        1 cgtttcacga gttcgtgatc tatcaactcc atgtcggcac ctttttcacg ccgaacctgc
       61 cgcacaaggg cggcacgttt ctcgatgtcg cgcgcaagct gccgtatctt gccgatcttg
      121 gcgtgaccgc gttgcaactc atgcccattc aggaatttca gaccagcttc agtctggggt
      181 acaacgggac cgactatttt tcgcctgaaa tggactttgc ggtggcggat gctgagctgc
      241 cggactatgt ggcagaggtg aacaagctgc tggatgcgaa ggggctgcgc cggtatcacg
      301 cggctgactt acacggggaa atgaatcagc tcaaggcgct ggtagacctg gcccatcttc
      361 acggtctggc ggtgctgctc gatgtggtct acaaccatgc gggtggggag ttcggcgatc
      421 aaagcctgta ttttttcgac cggcaggatc ccgccggcgg tcagtggaat tctctctact
      481 ttacggaccg gggccacgcg ggagggctgg tattcgattt ttccaagccg gaggtgcgcg
      541 actttctgat tcagaacgcc aaattttttc tcggcgagta ccggatcgat gggttccggt
      601 atgaccaggt gagcgtgatc gatcacgacg gtgccccgga cgggtggcgt ttctgccagg
      661 acctcacctc caccgtgcat gcgcaacgac cgaagacctt gcaccatgct gaatattggg
      721 atgtgaatcc ctttatcatc gcgccgccgc cggttggtgc cgggttcgat acgacgttga
      781 ccgatggact gcgaattgcc atacgcgatg tgattgccaa cgccagcgct ccggacgaac
      841 ggccgctcca catgacgcag ctggcgcaga gtctgtggcc tcagggtttt tctgagtcat
      901 ggcgtttcgt gcaggggccg gagaatcacg acatcgtcta taacggtcga gaagcgcgta
      961 tagcgcggct gggcgatccg gaccatcccc ggtcctggtt tgcgaggagt cgggcgcgtg
     1021 tcgcgaccgg tctcagtctg acggcaccgg ggattccgat gttgttcatg ggacaagagt
     1081 tcttggagga caaacagtgg tcggacgact ttgtgtccca ccgcgatctg ctgctgtatt
     1141 gggcgggcct cgatcagggc gacaggcaga tgctcgatca cctgcggttc acgcgggaac
     1201 tgttggcctt acgccgtctc ttgcctgcat tgcgcggccg tgggttccgt gcgacccata
     1261 tgcatgacca gaatcgggtg ctggctttcc atcgttgggt ggagggggaa ggtcaggacg
     1321 tgatggtcat tctgcacctg gccaacttca ctcgtgtcgg atatcaaatc ggcttgcctg
     1381 gcgagggagc ctggcgtgaa gtgttcaaca gcgatgtgta tgaaaattgg gtcaatgccg
     1441 gcgtggcggg caataacggc cgcgtcctgg ccaacccgca gccgcttcat ggttttgggt
     1501 actccgccgg cctcgtcctg cccgcaaaca gcatcctgat tttcgcccgg tagccatctg
     1561 gtgttcagtg cgtgagtctc ctgtattccc gcgactgtca tgcctgccag ctcctccgct
     1621 cgcgacgcca taaggctttt gcgtagcaca gacgacctca ccgtgcggcc gatattccac
     1681 ccggaattag caccatcggc gacagacgcc cccacgtacc ggcgatacag tgacggagtc
     1741 cggcaaagaa ctcatgtgtg gtcgtgccat ggcacgggag aggaggtcat gatgactcgg
     1801 aaaatcctgg cgactctcat gtttgtgtgg ggcatcggac tagtcagcct ggctcaggca
     1861 tttcaaactg cagtggtctt tcccgtcagt tcagtcgagc atgtggattc acgcaatcaa
     1921 gtcctcacgt tcaaaacgca ggatggacag gtgcgcatgt tccgggtggc ccaatctgtg
     1981 ggcatgaagc gggaatcgtt ggcgaagggt gacctcgtgc gtatcgaagt cgatctcgac
     2041 gaccagatcg tcaatatcgt gaagttgact cagacctcac tgtccgatcg cgaagcgctg
     2101 cgtcgtaaat gagtctccat ccatgagtca atgtgatagg cactcccgcg accgggagcg
     2161 ggaggctagg tggtccacca ggtgacaaca gggggatgtg gcctcgtgcg gaggggccgt
     2221 ggatgaaccc accggtctac cagccgaagt cctgccctgc gaccataatc cgcggcgatg
     2281 gccgcttcgt cgctcccctg attcaccata aagaagagac cttgtgacgt gagattgcga
     2341 gcgatcctgt cgaacagtcg ttcaggggaa aacaccgtga gtggcagtcg ccacgccagt
     2401 acaggcgatg gtgtcacgaa cggaaaccag gcggtgatca cgtccgcctt ggtctccacc
     2461 tcgcaatagt tcgcgaccag aaatgaagtc tgcgaaagcc ccgcgatata tccacgggcg
     2521 gcatcataac ggctgtaccc cgtcgggtag agccggaatc cctccacatc cacacccgtc
     2581 aacgttgcag gacggaagaa cgcgtgcaag gtgcgcgcat accaaaagtt tgcgcagccc
     2641 acatcggcaa cgacgcctcc ctgagggacc ggccggttcg tgtccgtcca tgcctggtgc
     2701 aacagatcga gataggcata gttggccagc gcggtatgct ggccgtagtg agtttcgaag
     2761 cgcaggtcat atcgcacctg caattcattg atgcgcgcga tctgccagga ggtgagtccg
     2821 gcaaggcagc cgatcggttt ttcatgatac ggctgtctcg accaccggat acgttccgac
     2881 aggcgaaacc acagctcatg atagagcgag cggcagcgtt gaaggagaga atagtggtcg
     2941 tccggcggcc atgcgcgtga gatgtccggt gtcgtcgcca taccgggaaa gtgtctcgta
     3001 ggtcgccggc ttttccaatg aaaaacgcaa tggagaccat cggctcacgc gagtggatgc
     3061 ctgcgggcca ctcattcgga ctcctgccgg atcaactcct tcaccatcct ccggtgcgga
     3121 ataccagcat ccatgaatag ctcgccgatc acgtggaatc ccgcacgttc atagaacccg
     3181 atcgcgtgag tttgggccga cagcgtcacg cgtgtgagcc cttgtcgcac tgcgcactcg
     3241 agtaacgcct caagcaacgc acggcccgcg ccccgaccac gccaatcttt gaggacggcc
     3301 atcctgccga tcgtgccctg tgcttgcatg cgcgcggtgc cgatggcgtc accctgttta
     3361 ttccaggcca gcacgtggac gcattgcggg tcgagtccat cccactccaa ctcttcgggc
     3421 accgcctgct cccggataaa gacggtctcg cggatagggc ggatcagttg ttcggcatgg
     3481 acccaatcca ccagttctac gcgaataggt tcgccagcct gcgtcacagc atgccctcca
     3541 aggtaaagcc cacaatgtgc cttcaatcgc ggtgggaatc aaccggaggg acagggcctg
     3601 cgtagtaaca cgtcagcgcg accgagggct ggtcggatca aattcgattc tgtttgggag
     3661 gattgccaat ggcatgttac cgtcatgatc aggagattgg tgcgcatgcc catggcgtgc
     3721 gcctgctctc cggtgacagg aaggaggatg acgagtgaac attcaacagc ggcgggctcg
     3781 agtcggatcg ctacggaaga cgcgggcagg gatgtggcgt gcctggtgcc atgccggcgc
     3841 gaaagaggca cggccgtccg tcttcagttg ggacttcctg cgctccgtct ggtctctgct
     3901 gtgtcgcatg acccggaaca agggggtgcg gccggatgag aaccgcttcg cgtggcaaga
     3961 acacatcggc tgcaccgctc cgcgttcttc cggaatctat gggcgcatga tctgatacca
     4021 atcggatctc tttctgcgag tacctccagc ccacaagctc tcttcctttc gccattggtt
     4081 tacggcgctg tcctggcaga acatccaccg gtcggcgtca acaaggtgat gccgtccttg
     4141 aatcaacctc gtttgcccag atcttttcat cgctgccgcc aaagatgcta ttgacgcgtc
     4201 gcccggttga ggcgaagatg cggcagcgtg gcgcaggttt tgctcaacaa ttctgtattc
     4261 ttggcaacag atcgaatgat aaggggcaac ctatgaaggt attgattgcg acggatggat
     4321 cgaagtacgg caagtgggcg accgagtggg tggcgagaat gccctttgcc gagaagccgg
     4381 aagtggctct gctgcatgtg acggatgtgg aagccttgcg cgcgccgttc atgtttcaac
     4441 cggtggtcat cggcaacgag ccgtttatcc aggaagaaat caagcgtatt gaagcacgag
     4501 gaaagacggc catcaccgaa gcgaaggccc agatggcttc cttgaagctc aagggaaagg
     4561 tgcagtcgga gcgggggccg gcgggtccca cgattctgga tcgggcgcct cgccgggacg
     4621 gcatcgtggc aatcgggagt cgcgggctgg acgcactcga ccggtttatg ctcggcagcg
     4681 tctcgacgca ggtcaccctg catgcgccct gctcggtgct gatcgtcaag gaagagccgc
     4741 gcccggtgag ccgtattctc tttgccgcgg atggatccaa ggcatcggag aaggccttac
     4801 ggtttctcct gaccaagctt cagcccgaag cccgggaagg atttgagccg atcgatgtgg
     4861 tggtgatgca tgcgatgccg ttcctgaagt atccggagct caaagaggcc ggtgcgcgtc
     4921 tggtggaaca atgtgccaac aaattgatca aggccgggta tgtggtcgat gaagtggtgc
     4981 aactcggtaa acccgccgac gagatcttga aagtggcatc caagaagaaa gtggatttga
     5041 ttgtgaccgg tgccaagggg atgggagcgg tcgcccgctt cctgttgggg agcgtctcca
     5101 ccaaggtcgt gcagcacagt acctgttcgg ttctcgtcgt ccggtaaggt gcgactctcc
     5161 agcggcgcca tcttggagaa cagaagaccg ctgtggcatt tcaccccgta tccctcccgc
     5221 aagcgtagca aaacgctgcc atgggtgtgt cattccttgt gaaatttcgg ctcgcccgcg
     5281 gcatggtccc tgctgtggtt atacacggca gggacatgca gaacagacaa tggacacatg
     5341 accgatcgag tggcggctct cacatgtgag ttcagacggc tgcttccgcg gccggcctgt
     5401 tcgcttcacc tcgcgggtcg cgcgctcctg gtagtgctgc tgatggggca ggtcgggtgt
     5461 gatcgagatc cgggggtgcc gccggccgct aagcctgtga gcagagcggc tgatggaatc
     5521 gtacaactga ccgcggcaga aatcgggcgg gctggcattc aggtgcagag agcggcaaaa
     5581 gaaccctttg tcctgcatcg cgaatttccg gcgaccgtgc aagccaatga aaacgaattg
     5641 gcagaagtca ccacgttgat tcggggccgc gtggtagacg tattggtcga tgcgggcaag
     5701 gatgtgaaga agggtgagcg gctggccttg ctcgacagca ctgatctggg catggccgag
     5761 ggggtgtatc tcaaggcgac ggcgcggcaa catgaagcgc agctagcgca tgaacgggcg
     5821 gccaatctgc atgagcatcg tgccatcagt ctggctgagt tgcaacgccg ggaagcggaa
     5881 atgaaaacgg cgcaggccga ggcccgagag gcgggacatc ggctccggct gctgggcgtg
     5941 ccggaccagg agattcagcg gctggaacgg gagcgaacca ttcgttccga catcgcgatc
     6001 cgggcgccgt tcgcgggccg cgtgatcatg cgaaatctca cgcgcggcga agtcgtggaa
     6061 acctcgcgga actgtttcac catcgccgac ttgtcagatg tctgggtgat cgccagtgtg
     6121 ccggagaaag acgtgcagtt cattcatccc aaccagacgg tccatgtcat cgtcgcggct
     6181 tatccccata gcctgttctc cgggcgcatg acctacatca gcgatgtgct cgatccgacc
     6241 acccgaacga tgcgcatccg tgtgaccgtg ccgaatccgg atcgtgcgtt gaagccggaa
     6301 atgtttgcca tggttcgcgt cgatgcctcg ccacagcccg ctgtgctgac tgtgccgttg
     6361 tcggcaatac agcaggaggg cgggggcaaa atgttgttcg tgcggcaagg ggagaatcga
     6421 ttcgcgccgc gccgcgttca gttgggcgat gaacaagatg ggcaggtcat tgttctggag
     6481 gggctgcggg aaggggagga cgtggtcgtc aagggagcct ttgcgatcaa gtctgaactc
     6541 gatatccaca aaatcgagcc cactcaatga ttgctgcgct gctcgaattc tccctgcgcc
     6601 aacgaatcct cattctcggc ctggcctgct tctgcgccgc ggccggtgtc ttcgccttcc
     6661 agtcgattcc catcgacgcc tatcccgatg tgaccaacgt gcaagtgcag gtgctgacgg
     6721 aagcggctgg actctccccg gtcgaagtgg aacggttcat tacctatccc atcgaactgc
     6781 aaatgaacgg attgccgggc ctgacggaaa ttcgctccat ctccaagttc gcgctctctc
     6841 agctgacggt ggtgttcgag gacgatgtgg atgtgtactt tgccaggcag ctcgtgctgg
     6901 aacgaatcat ggcggtgcgc gagcggttgc cggccggcat ggagcctgtt ctggcccccg
     6961 tgaccaccgg gcttggagaa atctaccagt attacctcga gggcgggacg aagccggata
     7021 cgaatgcggc gctggtggaa gaagcgctga cgaaccagcg gaccatacag gactgggtgc
     7081 tacgtcccct gctgaagggc gtgccgggcg tcattgatgt gaacagcctc ggcgggttcg
     7141 tgaaacagta tcaagtcctg gtggatccgg acaggctacg caagtacagc cttacgctgc
     7201 atgacgtctt cgatgccgtg gaacgcaaca atgcgaacgc cggaggaaat gtccttgaac
     7261 gccatgctga acgcgccatt gtgcgcgggt tgggcctcat caaaagcctg gccgatatcg
     7321 agcggatcgt cgtcaaggag gcgggcggcg tgccggtgtt tgtgcgtgat gtggcggagg
     7381 ttcgcatcgg ccatgcggta cgccacggcg ccgccgtgct gaacggcgaa cgggaggtgg
     7441 tggccggcac cgtgttgatg ctgcgaggcg gcaacgcgcg tgaagtggtt caggcggtga
     7501 aacaacgggc ggaatccatc gagcgtcagg gaattctgcc cggcggactg accctcgtgc
     7561 ctttttatga tcgcattgag ctcgtgacgg ccgccatcca caccgtgcgc gacgcattga
     7621 tcgaaggtgt cgtgctggtt gcgctggtct tttttctctt cctcggtcat gtgcgaagcg
     7681 cgatcgtcgt gacggtctcg ctgctggtca cgccgttgat cacgttcgtg gtgatgcagc
     7741 gtgtgggact ctccgccaac ctgatgacct tgggcggact ggcaatcggc atcggagaaa
     7801 tcgccgacgg ctcgctggtc gtggtcgaaa atatttatcg ccacttgtca gagaaccggc
     7861 tgcaggaacg atcccgctta gaggtcgtgc tgcgggcgac gaatgaggtc gggcggccga
     7921 tcctgttcgg cattctcatc attagcgtcg tctttctgcc cttgatgacc ctgcacggca
     7981 tggaaggcaa aatgttcgcg cccctggcct atacactcgt catttccctg ttggcctcgg
     8041 tgatcgtgac actgaccctg tccccggtcc tcgcctctct gatcttacac ggcgatcatc
     8101 cggaggagac tcgtctcacc cgctggatga aggcgcagta tcaaccggtg cttcggtgga
     8161 cgctggacca tcgcgccgcc gtgctgctga gttcaacggc gatcgtgctg gccagcctga
     8221 gcctgttgcc ttccgtcggc cgcgaattca ttccgatctt ggaggagggg gcactgaccc
     8281 cgcagatcgt gcgcctgccg agtgtgtcgt tggacgaatc gatcgccata gaaaaacaaa
     8341 cccaaaaagt catgctggag tttccggaag tgcggatgtc agtcagtaag atcggccggc
     8401 cggacatcgc ggtggggccg gaagagccca atgaaagcga cccgatcgtg gcgctccgcc
     8461 cgcgtgaggc gtggatcacc gcgcagacgc aagccaagct ggtcgatgct atcaggacac
     8521 gtctggcgga aatccccggt atttccgtgc tgatgagcca gccgattcag gagcgggtgg
     8581 acgagctgat ctccggcatc aggaccgaat gcgccatcaa gttgttcggc gacgatctcg
     8641 atctgctgta tcagcatgcc gaacggatcg ccgatctcat gcggacgatc gacggagtca
     8701 aagacgtcaa ggtcgaacag gtcgccggtc agccgtacct cacgatcgat atcaatcgag
     8761 aaaagatcgc tcgatacggg atcaacgtgt ccgatgtgca agacatcatc agcacggcgg
     8821 tgggcggaag accggcgacc caggtctatg aaggcgagcg gcggtttcaa ctggtccttc
     8881 gatttcctga atcgtcccgc aacagtatcg gcgcgatagg agatattcga gtacgatcgg
     8941 cgtccggtgc cccgattcct ttgagcgaac tggcggcgat cgagatgcgg gaaggtccgg
     9001 cgcgaatcag ccgggaacag gcgaagcggc ggatctatat cgggttcaat gttgtggggc
     9061 gggacatcgg cggcgtcgtg gatgaaggcc ggaagcgctt ggccgagcag ctccgattgc
     9121 cgcaagggta taccgtggcc tggggcggcg catttgagaa tatggaacgg gcgaatgcgc
     9181 ggttgcttgt ggtggtgccg atcacgctcg gcctggtgtt ctttctgctc ttttgggcgt
     9241 tccattcgct gcggtatgcc gggttgatca ttctgaacct gcccttcgcg ttgatcggag
     9301 gcgtggtttc cctgtggctg agcgaccagt atttgagcgt gccggcctcg atcggcttca
     9361 tcgaactgtt cggcttggcg gtcggcaatg gcatcgtgct cgtgtcgtac atcaatcaat
     9421 tgcggaacga agggcagcag acagaggcgg caatcatctc gggctgcgtc ctccggcttc
     9481 gaccggtcgt gatgacgatg atgacgaccc ttctggggct tctgcctctg gtgttggcgc
     9541 aggggatcgg cgcggaagtg cagcggccgt tggcgacggt ggtgatcggc ggcctgttca
     9601 cgtcgacggc tttgaccctc ttggtgctcc ctgccttgta ccgtacgttt gccgaacagg
     9661 acgtgacgaa ggaacatgct cccgagtggg tgtagcccga tgccggtgtg gaaccatgct
     9721 caagcagaca ccattctggt ctctttccgc cgatgatgtg ttgcggcaat tgtccgccac
     9781 accaaatgga ttggcgacag cggaggttga cgcgcggcag gccacctctg catccgttcg
     9841 gctgaagcct cagcgcaata gccggccgct gcgcttgctc atggcgcagt ttcgcagtcc
     9901 catcattctc attctcctct tcgcttccgg ggtctcgttt tttctcgctg agcatagtga
     9961 tgcgctcatc atcctgacca tcatcctcgt cagtgccctg ctgagtttct ggcaggaata
    10021 tagcgcggcc cgggcagtgg ccggcctatt agctcttgtg caaatcacgg ctcgggcatg
    10081 gcgggatggc gagctgcgcg acattccggc cgatcacatc gtgccaggcg acgtcattga
    10141 gctttccgcc gggtcgagcc taccgggtga cgccttggtg ctggaggcga aggatctgtt
    10201 cgtcgatgag gccaccttaa cgggagaaac gtatccggtc gagaagtcgg taggctcact
    10261 accggctcat gcgccgttgg cgaagcgtac caacagtctg tttctcggca cccatgtcgt
    10321 gagcgggcaa gcccgggttg tcgtcgtggc ggtgggaacc gacacggaat tcggccgcat
    10381 cgcccatcgc atggcgctca gggcgcccga gacggaattc gaacggggcg tgcggcgatt
    10441 cggctatctc ctgctcgaag tgacgctgct gctcgtgctc gcgatttttg ccgtcaacgt
    10501 ctatcttgaa cggccggccc ttgaatcctt cctcttttcc atggccctgg cggtgggact
    10561 cacgccgcag ttgctgccgg ccatcatcag tgtgaacctc tcgcacggcg cgaaacgcat
    10621 ggcccaacag aacgtcgtgg tcaagcgcct ggcgtcgatc gaaaatttcg ggagtatgaa
    10681 cgtgctgtgc tccgacaaga cggggacctt gaccgaaggc tccatgcgac tgcatgccgc
    10741 gctggatctg gatggtcagc cgagcgagcg cgtgctgttc catgcccaca tgaacgcgat
    10801 gttcgaaacc gggtttccga atcccttgga tgaggccctg cggcagcatc gttccttcga
    10861 tctcggcagc taccgtaagc tggaagagga accgtacgat tttgttcgga agcggctgtc
    10921 ggtgttggtc gccactccgc agacccatct gctgattacg aaaggcgcgg ttgaaagtat
    10981 gctgtcggtg tgtctgcatg ccgagcagca gaacgggtcc ctcatgccga tggatcaggt
    11041 tcgcgcatcg atcagggagc aggtgcgggg gctcagcagc gagggctacc gtaccatcgg
    11101 agtggcctat cgggatttgg gagccatcga ccgggtttcc aaagagcatg aaacgggaat
    11161 gaccttcctg ggcctgctag tgtttgtgga tccgccgaag gccggtattg tcgacacgat
    11221 cgcgctgctc cgcaagctgg gggtgtcgtt aaagatggtg acgggggatc aaggactggt
    11281 ggcggcccat gtggggcgac aggtcggcct ggccaatccg cgcttgctga ccgggagtga
    11341 cttgcgaggc atgaccgacg acgcgcttcg ggcccgggcc aacgaagtgg acatttttgc
    11401 ggaaattgaa ccgaatcaaa aggagcgcat catccgtgcg ctacgcggtt ccgggaatgt
    11461 ggtgggctac ctcggcgacg gcattaacga tgcgcctgcc ctccacgccg cggatgtggg
    11521 cctttcggtc gatggcgctg tcgacgtggc caaggaagcc gccgatcttg tgctgttgga
    11581 acatgatttg agcgtattgg tggaaggcgt gcgggagggg cgacgcacgt ttgccaacac
    11641 cctcaagtat gtgttcatgg cgacaagcgc gaatttcggc aacatgttca gcatggccgg
    11701 tgcctcgctg ttcttgccct ttctgccgtt actcccgaaa caaatcttgc tgacgaatgt
    11761 gttgacggac attcctgaaa tgacgatcgc caccgatcat gtggactctg aattgctcga
    11821 tcgaccgcgc cgctgggata ttccgttcat ccggcggttc atgctgacct tcggattggt
    11881 gagctcgttc ttcgattacc tcacgtttgg catcttgttg ttcctgctgc acgccacgac
    11941 cgcccaattc cgtaccggct ggttcgtcga atcggtgcta tccgcgtcgt tgattgttct
    12001 ggtcattcgt acgcgccgcc cttgcgtgac cagccgcccg tctgcgccct tgttgtggtc
    12061 aacgttgctg attgcactgg tcacgatcct gttaccgata acgccgatgg gttcatttct
    12121 gggttttgaa ccgttgccgg ctgtcttttg ggtcgcgctg ctcggcattc tcctggccta
    12181 tgtcgcggcg gcggagctgg cgaaaacgtt cttttaccgg caggtcaata acggccgctg
    12241 atcaggcgac gcgacgcgct tccataattc ctacgtaccg cccaagccag acaatcgatc
    12301 atctccttga ctgtacgtgg tgatgtagta caaccagaga gaggttattc gttcaacatt
    12361 cttccacgcc caatccgcac gaggacgccc atgcccggtt ctccgcactc ccccaatggt
    12421 ctctcgtatg atccgcaggt gctcctgaat tcccagccgg tcatcgtgac cgtgatcgat
    12481 cctgccgacc attccgtgca gttccaaaac cagaccggtc tgggagcgtt cggcgacatg
    12541 gccggctcgc cctgttatga aaaaattgcg ggaaagacgg cgccgtgcga attctgccgc
    12601 atgcctgagg cgttgagccg agatggagtg gtctctgagc aggtggaaat gccgaacggc
    12661 cgccatttgc tggtgcattg ggcgaaggcc ccgacaaaag acggccgtgt ccatgtcatt
    12721 gaaaccatca tcgatctgac caagcgcaaa caggacgaac aaagcctgcg gcaatcgcag
    12781 aaaatggaag cgctcggccg attggccgcg ggtatcgccc atgacttcaa taatttgctc
    12841 atggtcgtca tcggccatgc gcaccgagtc gcgcagcaac tgggatccca tccgctgcat
    12901 catgaagtgg aaatgatcgg ccaggccggc acgcgagccg cggcgctgac gaagaaattg
    12961 ttgacgttca gtcgacgcca ggtgctcgag cagcgtgacg tgccgctcaa caccctgatt
    13021 cgcgacatgg aagacatttt gcgtcgattg atcggtgagc agatccagac ggtgattgtg
    13081 ctccatcccc aggcgggcca cgtcttgggc gaccccgtgc agatggagca ggtattgctg
    13141 aatctggccc tgaacgcccg cgatgccatg gcagaagggg gcattctgac gatcgagacc
    13201 ggcaataccg atgtcgacga gcagtatgtg cgcactcatc ccggcgcggt tcccggtcct
    13261 tacgttaccc tggtggtcga agatacggga tgcgggatcg atgccgagac cctcgcccat
    13321 atttttgaac cctttttcac cacgaaggaa tttgaaaagg gaaccgggtt ggggctggct
    13381 accgtctatg gcatcgtgaa ggaaagccgc gggtatattg acgtcacgag ccaacctgga
    13441 cggggtacgc gctttacggt gatgttgcca cgcgtgcagt cgcaaacatc ggaagctgaa
    13501 cagccggcat cctcacgacc gcttccggcg acttgtgcca ccattctcct ggtcgaagac
    13561 gatgagggca ttcgacggct tgtggcggcc gtgctgcgtg atcaggggta tgaagtgttg
    13621 gcggcggcgg acggcgtcga ggcgctgcag atgttgcaac tacgcaaggg cggctgcgat
    13681 ctcctcatca ctgacgtgat cctgccacgt atgaaggcct cggttttggc gcagggagcc
    13741 aggacgatgt ttcccaagat caaggtgctc tatatctccg gatatgccgg tgacatgttg
    13801 gtatctcatg gagtggatgc acaagcagcc tacctccaaa aaccatttat gccgcaggcc
    13861 attatcgaaa aagtggccga attaatcccg cccgctcctg cgcagtaacg agctcgtatc
    13921 tattgcgcgt ggatggtcgt tcaccgatcc gcttgcgtga tctccttcga gagtgtttcg
    13981 tcttcttcgt accgcgcccc gcgttttgcc ccgcgcgcca ctgggcgacg gattgcaccc
    14041 atgcgtcatg gccaaacagg gcagaactca agattctggc tggtactcgg tggttgtgcg
    14101 aggcagggaa cttatttcca ggagacgccg ggaagagaaa tgacccgttc cgtttcggct
    14161 gtcgactgcg cgtccagaag aaggtcgtac acgagtttga cccggctgcg cgtttctgcc
    14221 gaaagcgcca ggaattccac gccgaaggca tccttactcg tccacgtgac gacggcgggc
    14281 ttgatttcga tcggttcgat gcgatcggga agcgagatcc gcagcgtcaa tcgcatcccg
    14341 cagtgaacct tggcggcagt ctgttgaatc ttgcatccgc cgggtgaaaa atccttcacc
    14401 acaccctggc tggttccacg ggggccttgc acatcgatcg gccgattcat ctcgacacgc
    14461 gtattcttac gttttgacat cccttggtcc ttttgtaatc ggcgggtctt ccagggttcg
    14521 cacggtcctt agatagctgg ctgcatgagc gattccgtta atgttctaaa cgcgggatcc
    14581 agcatgaacc gaccgaagga tacatcctgc cgggggtctg gtatgggttc tcggtcgttg
    14641 ctgcgacgga agtgcagaaa tgcgtcgtgc agagattgca tggcccggtc acgttcattc
    14701 attccggcgt aggtgcgggc gagatcatag tgaaaggatg gattcgcggg ctcttgtgaa
    14761 agaccatagt cgatcgttgt tttagcgcgg gcgagatcac cgctcctgcg ataggcgaca
    14821 cccaggttat ggacgagcag acgccagaga gccttgtcca aagaggggtt cgtccgctca
    14881 agggcgacgg cccgttcata gtgcggaatg gcctgcttga attgtccctg cagatacggc
    14941 gcgctccctg cacgaaagta atccaggctg ccgggctggg ccgccgcaac gatggaaagg
    15001 ctctccagca ccttccgaag ttgcgaggcg tctactgttg tggtgccgct gtgtgacaga
    15061 tgaatgtcgg catagacgtt gtctttgttc gtacaggcaa agaggtgaac ctggtccgtc
    15121 cacccggcgc tggtctcata cagaagatat tcgaagaccg ggagttgatt gatctcggtt
    15181 cgtgtcggac ctgcatggga tggtgccgca gctgtttgtg cgatacggtg caaatgcgcg
    15241 aggcagccct gttcggtggc ctgtccggag acggcctcca gcgtgacgga gagctgcatg
    15301 gcggctgcgt cgttggtggc gagcacatag cgccggctgt ccggtttgac gccatccgct
    15361 tggacgcgat agccggtcat gtcgagactc acggcccagg tcttgtcgat gagggcgatg
    15421 cttcgctgat gcggaatctc ctcggcgaga ccggcaccgg atgccaagag agcatagccc
    15481 agtgcacttg caagcaactg tttcattgcc gatggcattc ccgctccctg tgcgctcttt
    15541 ctgccagctg attgtggcgt cactgtagtc taacgtcgag gtcttgcgga ccaaagcaaa
    15601 agtacaaaac cagcgtcctc tatgctgagc atcattggtt ttcgatcgga aacacgggtt
    15661 gatgctgtgg cgtcgtgcca caggatcagg agaggactgt cgcctttgac ggcagcacgg
    15721 cacacctcag ggagtgaccg tgtggcgaat atctcgcggg ccaccatcaa tcaatggcgg
    15781 tatcggcgtt gcatagtcct cgataaggat gtatccctat cctgggacaa ggcacgatgc
    15841 tgtgaagaga gaggtggcga tgcgcgaacg agacgtacag gcgatcggga cgaggcgagg
    15901 cgctcgtgta gggtggcgga cggtcttcgg gatcgttgct gtggccatga tggcacaagt
    15961 atcctctgct cctgcggtgg tgcacgtact tcctgatatg accgtgacgc gtgacagtgg
    16021 gaccgtcacc ggtgccaagg cggttcgtgc cgatcccacg ctgcaggaag tgctggcggc
    16081 attcgatcgt gcagaacgtg ccctggagaa gcaggacctg tcctcgctga tgcagtttta
    16141 tgcgccgggc tacaactatc acggcttaca cgtggaggat gtccagcgca tctggggtga
    16201 agtgttcgag cactatcgga cattgtcctc gacgcatctc ttttccgaga tgaagatcgt
    16261 gcgtgccgag gacgggttac gcgtcgagtt gacctgcact ggcggcttgt atggcgctga
    16321 cgtgcaatca ggcgcgcgca tcacgctgga tagctggttt caggaggtgc actatctcgt
    16381 gcgggacagg cagggatggc ggtttctggg aaatcgaggt gatgcaccgc gttcggcgcc
    16441 cttctcctca tccccgcacc atccgctgtt ctgatcgaga cgggccaggc ggtcaggctg
    16501 cggccgccga ggcggcagtg gccgcggtgc tgagcagctt tttgacgctg gcatgggcca
    16561 acaccacgtc cggagtgcgt tgcagggctt cgtaataact ccgttcgaac ccgtcgcctt
    16621 cttctgaagg gcgcagcatg tggcgggctt ccagcagaag gtcttgcgcc gtttcgatgg
    16681 tcgtgtcttc gtgatcttcc agcacgacat cgatgcgaac gacgagatgc gacaaggggt
    16741 gcagccaggc gaaccacggg tcattcatca ccagctgcaa aagctgaccg gccgaggaga
    16801 tgcgcccata gatgcgttca tacgtcagct gttcggagac gatgagggat ttgtgcaggc
    16861 cgagcaagcc gtgacgaagg tccttcaggc tggcacgaag gttatccgga agcggattcg
    16921 aagacgacca ggaggaacgt gtcatagatc acccacccta cgcaatccac agcttcaagc
    16981 gacggccgtt cctaccacag gaatgatgcc cccttcaggg ccactctccg tccttcgtgg
    17041 atactgtatc ggctggtttg cgccgggatt gagtccccgg gattgtgaaa tcgacgaaac
    17101 tccccctcga agccgcgtgg gcgagggcag gaacggctgt tttctccgca agttgaagtg
    17161 cttaggattg gggttggggg aagggggcta tccctaagga gccgcccccg cagggaagct
    17221 gaaagagatc ggaacgtatg gtcgaagccc gacagcatag ccagacccgg tggtacgcac
    17281 ggtcccttga cgatctcggc cgggagttcg gcgtcgatca ggcgctaggg ctggatgaag
    17341 atgaagccgc gcgccggctt accgcccatg gccctaatga attgcctgag gctgcaccgc
    17401 cttcgccctg gcatatgttg ggtgcgcaat tcagcagcct gatcatctgg gtgctgatcg
    17461 gcgccgctct cgtgtcgggt ttgctcggcg aatgggtgga cgcgtgcgcc atcttggcca
    17521 tcgtgctgct gaacgggttg ctgggattcg ttcaggaata tcgggcggag cgctcgctgg
    17581 cggcgctcaa gaccctggcg gtaacctatg cgcgggtcat tcgtgaaggg gtgcggcgca
    17641 ccctgtcatc aacggagctc gtccccggcg acatcatcga tgtcgaggcg ggcgaccatg
    17701 taccggcgga tgcgcgactc attcaggcca cagcgttcct tacgcaggaa gcggccttaa
    17761 ccggggagtc caccccggtt gaaaaaacca gcgggacgtt gagcgacggc gatatgcccg
    17821 tggctgacca gcgaaacatg ctgttcatgg gcacaacggt gaccggcggg aaagggcgcg
    17881 ccctgatcgt tgccaccggc agcggtaccg agctggggcg tattgcgacg ctgatgacca
    17941 cggtgcctgt cgagccaaca cctcttcagc ggcgcttgga acagttcggc catgtgttgt
    18001 tgtggttatc actggctatc gtggtcgtgg tgttcgggct tggcttgtgg cggggggagc
    18061 cgctgttcga gatgtttttg acggccgtga gcctggccgt ggcggccatt ccagaagggc
    18121 tgccggcgat tgtgacgacg acgctggccc tgggcgtgat gcgtatggtg agccgccatg
    18181 cgctgatccg ccgtctgccg gccgttgaaa ccctgggcgc ggcgacggtg atctgtacgg
    18241 acaaaaccgg caccttgacc aagaacgaaa tgacggtcac ccggctggcc gtcgatgacg
    18301 tggtctatga cgtaaccggg gagggatatg cgccggaggg cacgattgtc gatggcgatg
    18361 gccgcacggg agcgttgcga gcactgttgt ggagcgcagt cttatgcaac ggatcctcgt
    18421 tgcgggccgc agaaggcagc tggacggtgg tgggtgatcc cacggagggg gccttgttgg
    18481 ttgcgggcgg caagggcgga tggcggaaag aagcactcga acgcgcgcac cctctggtgg
    18541 gagaaatccc cttcgactcg gaacgcaaga tgatgacgat gattcgccga tcggaagggc
    18601 gtgccgtggc ctacgtgaag ggtgccccgg atatcctgct ggagcgatgc gattcgtata
    18661 tgacgaagtc gggggaagtc gcccccctgt ctgattcaat gcgccgttcg atggtatcga
    18721 tcaatcagca attcgctcag caagccctgc gggttgtcgc attggcgcaa cgcgtactcg
    18781 atccggatcc ggcgacattc gagtcgaaag gcattgagca gcaattggtg ttcctcgggt
    18841 tggctgcgat gaaagatcct ttacggccgg aagcgaaaca agcggttgag cgatgccggt
    18901 ccgcaggcat cacgaccgtg atgattaccg gggatcacaa ggagacggca ttggctatcg
    18961 gacgcgaggc ggggttcgca tccggctcga cgcaggccct ctccggcctg gaactgaacg
    19021 gattgactga tgcggagctg tcgtcccggg tgcgtgacgt gtgtgtctat gcgcgcgtgt
    19081 cggccgaaca caagctgcgc atcgtcaggg cctggcgcgc gcaaggcgca gtcgtggcga
    19141 tgaccggcga cggcgtgaat gatgcccctg cggtgcggga agcggatatc gggatcgcca
    19201 tgggcatgac aggaacggat gtgacgaaag acgcgtcgga catggtcgta accgacgata
    19261 actttgcgtc catcgccgcg gcggtcgagg aaggccggag catctacgag aacattcgca
    19321 aagccgtgca ctatttgctg tcgtgcaacc tgagtgaagt gctggtgatg ctgggcagca
    19381 cgttgttagg ctggccgttg ccgctgctgc ctatccacat tctctggatc aatctggtca
    19441 ccgacggatt tcctgcgctc gcgttagccg tggatccgaa agatcccgat gtgatgaagc
    19501 gcccaccgcg cgaccccaaa gcgccgctgc tggatcgcca acggttcgtg accgtgtgcg
    19561 ttcaaggcgc ggtgatggcg gcggcgacct tggcggtgtt cgggattgcc ctggtgatca
    19621 tgaaggacga ggtcacgttt gcgcgcacca tgacctttac gacgctggtg ctggttcagt
    19681 tcctccatgc gtttacctgt cgtcacgacc ggttctcgct gtttcagatt ggtatcacca
    19741 ccaatcggat gctggtggcc gccgtcctca tctcagccct tctgcaggct gggattctgt
    19801 tgagtccatg gggacaggat atctttcgag tcgttccgct acgactcgat gactggtggt
    19861 tggtcgcggc gtgcggaatg ctgccgttcg tggtgatgga actgtggaag gcctggagtc
    19921 gagtgcgaaa cggggccgcg cgcccgtcta cggcgtgagt ttgggcgctg cccagatcgt
    19981 ggtaagcggg gaggcctgcc ggctgagccc aaccggttgc gctccataca tgtccgtgat
    20041 cgtccgcagc agcccgtagt gattcgtctg ctcatggtag cgtcctgggc gcaccatcgg
    20101 acccacgaga atcgtgggaa tatggttgtc ggattttccg ttgtcctcgt cccacgtaat
    20161 gatcaacagg ctgttgtggg tctgcgccca gtctacgtag cgttcgaggc ggacctggag
    20221 ccagtgatct ccgcgttgaa tgcggtcggg atcgttgccg tcgtgcatat cgttttgttg
    20281 attgggcaca atgatgctga cggttggcaa ggtgctcaaa tccgtcggaa agtcggtcag
    20341 cgggcggttt tgcgcagccg ggacggtgtt gacaggagac gcttgccagt tcacccaggg
    20401 gttatgtttc cgcgcataag cgcctgcaat gcaatcgacg gcccccacgg cggggagatc
    20461 ttcggagtaa ccaataaacg actgtccggc ctgcttcaga gtgctggaca ggttcggagc
    20521 cgtcagcggg gtcggacaga cattcccatt gacgccttcc atggtgccgg caaacagcgc
    20581 gatgtagttc ggctggctcg gatgggttac ggcgtaggaa ttggtcagca gagcgccttt
    20641 acgtgccagc gcgtttaagt atggggcggc cggagagtcg ataatctggc cgaaggcatg
    20701 attctcttcg atcacgatca ccacatggtc cggcttgggc aagggcgaat gggctgaacc
    20761 gggggtggtc caggagagga agagtgcggt gaagacgagt gcgaagactg acctgttcat
    20821 cagggtctcc tgatcgatta caccgggagt tcgctgagtc gtagtgtgta gaggtagtgg
    20881 tacagcccct gttgctccag gagcgccgca tgggtaccgg attcgacgat cttgcctttg
    20941 ttcagtacca gaatgcggtc agcgcgttga atcgtgctga gtcgatgggc cacgacgaag
    21001 gtggtgcggt tgaccatgag ccgttccagg gcttcctgaa ccagccgctc cgattcggta
    21061 tccagcgccg aggtggcctc gtcgagcagc agaatccggg ggttcttgag aatcgctcgg
    21121 gcgatggcga cgcgttgccg ttggccgccg gacaagttga tgcccttttc gccgacgatc
    21181 gtctgataac catcaggaaa tcccacaatg aactcatggg catgggctgc agtgctggca
    21241 gcaatgattt cctcctcggt ggcatcctcg cgtccatagc gaatgttatc gcggatcggg
    21301 ccgccgaata agatcgtgtc ctgaggcacg agcgcgattt gccggtacag gctgtcgagc
    21361 cgcacgttct tgacgtcatg accgtcgatg gtgagccgtc ccgaggtcgg atcatagaat
    21421 cgatgcaaga ggttcatgat cgtcgttttg ccggagccgg tggggcccac gatcgcaatg
    21481 agttcaccgg gcttggcttc gaacgacacg tccgagagca cgggttgccg ggggtcgtag
    21541 gcgaagctca gattttccgc gcgcacgtgc ccgcgaaccg ccggcaattc aatcgcgcca
    21601 ggcgcgtccg caatttcggc atgggtatca aggatttcga acacgcgttg catcgcgcct
    21661 tgggcttctt tgatttgcgc aaagacgcgg gctgccgatc cgaacggtcc gatcagaatg
    21721 ccggcaaaga gcacgaacgc aaacaggtcg ccgggggaga ctgagccgtc gatgacctgg
    21781 cggccgccgt accacaagac gagggcggcg gcggcaaagg tgacgaagat gatcgtcggc
    21841 acgaaccagg ccatgatggt ggcacggcgc agtgacagct ccatcgcgga ctggacctga
    21901 gtcacgaacc gttcttcttc ccgtttcgtt tggacgaagg atttcacgac gcggatccct
    21961 gcaatgactt cttcgaccag cgtgctgacg gtagccgttt ggtcctggat tttggtagac
    22021 agcgcccgga gccgccgccc gaacagtttg gccacgacga ccagcatcgg gagcagcacc
    22081 agaatgagca ggcagagttg ccagttcatg gcgaacagga agcccgcgcc gccgatgaag
    22141 gtgacgattt gtttggcggc gtcgatgggt gtatcggtga cgacattttg gatcacggtg
    22201 acatcgttca tgaggcggga catcaattcg ccggttcggc gcttcgagaa gaagttgact
    22261 gacagcgtct cgagatgtgc gaagagatgg atccggaaat cggccatcac ccgttgggag
    22321 acccaggccg tgaggtaact gtgccccatc gaacataacc cctggatcaa gaccaagccc
    22381 aacaagagca ggatcatgtt tgtcatgcgc ggggcatcgt gctggaccgt gatgacgtcc
    22441 cacagagtgc cgcctaagcg aaggagggca aggttgatgg cagcaaccgc catgaccatg
    22501 acggcggcag cgatcatccg ggggacgtag ggcttgagga aggggtagaa gcgcttgaac
    22561 gagaacatcg gtgttacgac aattgtttca gggaggcagg cgcgtagcgt atcaccggtg
    22621 gtcgcggcgg tacgaagacg acatgacctt cgtgatgagg tgagagcgct agagctgagc
    22681 gattgactcg atcaggttct tgttgatcgc cacaaagtcc gatcgatcct tgtcattgat
    22741 caacacgtca gtcaagctga taaacagccg ctgctcttca ttgatcgcat cggacacacg
    22801 attacgcatg ggcgggatga ggaaatcccc gacgtagata cagttcaccg ttcgaacgcg
    22861 gatgcgaact ttttttcctt cgggcacgga tccctccgtc cattatgtcg tgatgggctc
    22921 agctcttgcg acgcaagaat ttctgacgcc ggcgcaactt cttatacttg tgcttgcgca
    22981 tcttcttccg gcgtttcttg aggacactcg ccatacattc aatctccaac tgctaggcgc
    23041 gggagtctag tgggtttcag
//
LOCUS       DAOQEW010000044        22500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_557_length_22500_cov_8.014346, whole genome shotgun sequence.
ACCESSION   DAOQEW010000044 DAOQEW010000000
VERSION     DAOQEW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22500
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_22500_cov_8.014346"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1236
                     /locus_tag="PLS35_06925"
     CDS             190..1236
                     /locus_tag="PLS35_06925"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18426.1"
                     /translation="MTDAETSPTVLSSREIQLHVLGLVLLLGVLGLWYWFSTGARVVP
                     TLIELLKDDDPSTRIVAAEQLGQVGPAARSAVPQLLSQATQDGSQHANTTAATALKWI
                     DLTAARRVMTHFVPRLQDPDVQLRRTACAVIGSLGPIAKPAVPALLAVTHDADALIRR
                     NALIALASIGIPSLPIAAALVDGLRDSSSLVRQTAVAQFAFTVPPSPDAVAVLTPMLN
                     DPDKGIATLARTALDKPRAGDAAHIESLGMMLGHSSARDYALHQLAQLGPAAHDALPP
                     VLALLEDEQPLIRYLAAESIGGMGAVAKQALPALQRHQDSDLVVRAAIAEAVSAIEAG
                     AVVNAEPLSKELPR"
     gene            1233..1733
                     /locus_tag="PLS35_06930"
     CDS             1233..1733
                     /locus_tag="PLS35_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18427.1"
                     /translation="MTDIPRQTSPRLRMMARLGVWISLLGGLLLPWIIMLTVEILVRD
                     IPYPRAWRSFTLHLFAPGYNFFFVGVLTALPFVILALIILFHLGTAPANNALIAYRRA
                     LGLFSAGLSMALIAAWVHVDVLIHPDAQGALAYFYLPILLLMMLPFGYGLGRALAKIF
                     LPRLSG"
     gene            1809..2783
                     /locus_tag="PLS35_06935"
     CDS             1809..2783
                     /locus_tag="PLS35_06935"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNI18428.1"
                     /translation="MIRLLPCLILLVLCHSTSLAASKYEDSVKELAEGVAAEAIKMKK
                     SRLALLDFVDSKGDVTPIGQFLAEELATHLLVAGELKVVDRKLLIATMEKHQLTHLDT
                     SQAKAAKKVAKALRADLYVTGAYLEIPEGIQVTAKLIGPYTVYPVGASRTIVPKSGPL
                     AALLKQAAAPAPATAAVVTDSAPVLSAHENDLYKFTILSVTRQDDLIGLDLVFTNRTA
                     HPVKIGCQLLDTYLVDEHGEQWKQNVAQNREGLCTRGMELNPKRQQRASLSFLATNKP
                     PTGPITLHYHETSPRADRIITLDGLKIASAPVVEPGAPAAQESTPPTP"
     gene            2818..3612
                     /gene="thiD"
                     /locus_tag="PLS35_06940"
     CDS             2818..3612
                     /gene="thiD"
                     /locus_tag="PLS35_06940"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNI18429.1"
                     /translation="MIKQVLSIAGSDSGGGAGIQADLKAMSANGVYAMSVITAITAQN
                     TEEVADVFELPTSIIAAQLDAIFDDFDVAAVKTGMLSSAEIIRTVVKLLKPQQVKHLV
                     VDPVMIAKGGQALLKPDAIDTLKSALFPLALLVTPNVHEAQQLSGIEITSLADARRAA
                     KVIHQFGCANVLIKGGHLQKERGTDLLYDGRFFNIFKGEYIDTPHTHGTGCTFASAIA
                     AHLARGKSVSDAVQTAKTYLTEAIRHGLAIGHGKGPTNHFYFLNQG"
     gene            3618..4907
                     /locus_tag="PLS35_06945"
     CDS             3618..4907
                     /locus_tag="PLS35_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18430.1"
                     /translation="MSRGLGSPLSFLLAGWGWVLLTALLGLAAFGGIVRGGPLPSGLR
                     LLHVHGVLIGGVLQLMTGLAIAAHDLSGHEGKRTTHRLLFLSMNLTAVGFAAGAWFRN
                     PTLTLAAGFLLAALFVPMARHMLTALRTCSGWTPLVHVYFGISFAGLFGSMVLGGLLA
                     DAWYPTWHGILRLGHLHAGLLLFLILGAVGTIQLTLPMMVHRPLHSPGMGQAVMLLLP
                     ASAAGLITGFVLSSVHIQLAAGTVLLIALALCCINLLRTWSQAGQPGSAATDHLLTAL
                     SFLLIMTFIGLAFGINVLSTPPAMPYGTLHLVAYTHTAFLGFLLQSMVGGLSFALPSL
                     LATSRIANQKKRGTYREDLERIMNRWRAAQVATVSFGSLGLVLVASLTWNLPLSSPWL
                     QTSTWISLGLLLFHFTMVTVKLAQATGAQPSHEGAAK"
     gene            4888..5727
                     /locus_tag="PLS35_06950"
     CDS             4888..5727
                     /locus_tag="PLS35_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI18431.1"
                     /translation="MKERRNNRHHFTTMEEALSFHDQAGHRIAAVLATPPQSTDSVAV
                     LCHGFLSHKNSTSNQALAELLKTRGIATLRFDCFGHGESDGPFAKLTTSIGVTQARAA
                     LTYAMTRGYRRLALVGSSFGGLVSLLAASEWTDLHNSAPEDVPPLACLALKCPVVDFG
                     EELRLELGEDGLQEWQRTNTIPDLHGGPSRLPLDYAFYQDCLNRIAYEPARAIAAPTL
                     IIQGDHDEYVPLHQSQRLYASLAGPKHLEILPGADHRFTNPSDFQLMLTLLTDWIAAP
                     PAA"
     gene            5988..7781
                     /locus_tag="PLS35_06955"
     CDS             5988..7781
                     /locus_tag="PLS35_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNI18432.1"
                     /translation="MKTPKSVLVIGVAGCLSSALCSTALPPTAAAADTTLSLLQQSST
                     DAVAIMAQNSGTAPIFTSPTALVLSAQKGQTAVGTLNLKKSSTDQHSYYLSTNQGWVW
                     MNPPYGSTQTITTETDQLVITAQTANLAAGTHSAVVYIVDSGPNNFTNMLRIPVTLTV
                     TATAVTPPPPPPPAAVPPAIIPTPSPVAVPPPPAPTPVVAPVVTGTAITSSPAALALS
                     AVKGQTAVGTLSLRKGGTDQHSYYLSTNQGWVWMNPPYGSTQTITTETDQLVITAQTA
                     NLAAGTYSAVVYVVQSGPNNFQNMLRIPVTLTVTASAVAPPPPPPAAVPPAIVPAPKP
                     VVLAPPPPPAPAVVPPPPAPAPPAPAPVTVATGPIQVTPSSLSLTSANNVGTLTLRKT
                     GTDQHAYYLSTNQGWIWMNPPYGSTQTITTESDQLVITAQTAGLAAGTYSGVVYIVES
                     GPNNFANTLRIPVTFTVAAGQTASSTPTTPTQPTVASPPPPPPSPPSPVPVTTTPTPT
                     SSASSTAPKTASATVSWNANTESDLAGYRIYVGTKSGSYGVVGPFEVTNGTSFTVPNL
                     PLGATYFFAVSAFDKSGNESAKSAEVSKSLF"
     gene            complement(7824..8708)
                     /locus_tag="PLS35_06960"
     CDS             complement(7824..8708)
                     /locus_tag="PLS35_06960"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNI18433.1"
                     /translation="MSGVCPARSIFIATLRCVHSALLGCLLAMALLYPAESTAGILEQ
                     LRKPRPSSELQVHSDVPMIMIPEGVFRMGADGTDALEDEKPQHDVWLSRFEIDRHEVT
                     TGHYAEFLASTKRPAPWQWESLDLSQHGDRPVIGVSWFDADAYCRWRGTRLPTEAEWE
                     KAARGTDGRLFPWGNHSPQEETANFGLGARFSYSQVLAPVERYEQGRSPFGLYQMAGN
                     AGEWVADWYGAHYYENGPRQNPSGPDAGAFRVVRGGSWSDLPKYLLTYGRFKLPPETR
                     NSYTGFRCARDGGVLASP"
     gene            complement(8759..9421)
                     /locus_tag="PLS35_06965"
     CDS             complement(8759..9421)
                     /locus_tag="PLS35_06965"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI18434.1"
                     /translation="MMKRVLEPELMDDVAQALAYAKADFAEENQGFVDRFREYFPAWD
                     GGHVVDLGCGPGDIPIRFLRAYPHARVTAVDASRPMLDLAADAIAGAGLTDHITLRCE
                     RFQSLVLPEQADALLSNSLLHHVPNPLQFWFRLKQLAKPGACILVMDLLRPDSPESAQ
                     ALVEQYAADEDPILKRDFYHSLLAAFTEDDVAAQLAEMNLSRLLIDVPDDRHWVVGGI
                     IP"
     gene            complement(9449..9940)
                     /locus_tag="PLS35_06970"
     CDS             complement(9449..9940)
                     /locus_tag="PLS35_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNI18435.1"
                     /translation="MTRSATPYVHQISVSDGGVPKLAVPSARITIDGLAGDRQRNRKY
                     HGGKDRAVCLFSLERIEALRAEGHTIGPGSSGENLTVAGLDWQHMAPGDQLTIGEHVR
                     LELTSYTTPCRLNAPWFKDEDFRRIAQEVHPGWSRLYARVLNEGVVKPGDVVVVDACP
                     GER"
     gene            complement(9948..10883)
                     /locus_tag="PLS35_06975"
     CDS             complement(9948..10883)
                     /locus_tag="PLS35_06975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNI18436.1"
                     /translation="MTSTSKVDQSSSSDSAAVQTGDWIQVDVRTSLDAGELLGMLNDP
                     DVTGAWQEGDEVHLYWPAGRCGPDTWQGLKRALTQLGHPDPDHAVTINHLANQDWNAQ
                     WSRLVEPIKIGRVLIRPSWRTVSLNSDEIELIIDPKQAFGTGHHATTQLLIEWLQQAV
                     RPDDRVLDVGTGSGILAMVALRLGATEALGVDFDAVAVDCAREYAQVNGFDTRLTLAV
                     GQAQAPQAHDAFDPTLVLANLDRQTLLTSADSLCAYAVRGARLFLSGLLAEQRSEIEA
                     AYATRGVYMKAVRERDGWLALEATAMETCEGGLCS"
     gene            10913..12796
                     /locus_tag="PLS35_06980"
     CDS             10913..12796
                     /locus_tag="PLS35_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18437.1"
                     /translation="MNPVTSAELKPSREQHLVHALRRAVHLETNGRRVSARFTTARLT
                     IFAATLLGSIIPHKLGWTLFGNLVLASGFVLFLIVAWYHNRLEDRLHRLRRWQGIKRV
                     HLARLSLQWAEIPLREVTVPERHGYARDLDLTGPRSLLHLIDHSISSQGRQRLASWFL
                     DQPPLAAPWSSRQALIRELTPLSLLRDRLVLEAQAVEEADIDGRRLLAVLQKRVDDSG
                     LMPMLLTESALAVTTALLLLADLISGLGNYWMLSFGLYAFLFLSVRSRSEEIFDHAQS
                     LHLQLERLSAVLQYLERRSYRSTPHLAKLCAPLMQQDSSPSRSLKQAASLMNRLSVRA
                     HPLVHYLINAVGPWDLFHTYRLQQLQDRIASVAPLWFELLAELDAASSLATFAHLHPN
                     YLWPTLHHAGADGQDNGDAPELAAEGLAHPLIPAAVRVANQVTLQGRGRIFLVTGSNM
                     SGKSTFLRTIGINTCLAQAGAPVCATAFRWSLVRIGSCIRVDDSLDGGLSFFYAEVKR
                     LKTILDSTRDRQGPPVLWLIDEVFKGTNNRERLIGGKALITALAGGNGFGLVTTHDLE
                     LAELEKQLPTVTNVHFQETVVAGALHFDYRLKPGPCPTTNALRIMALEGLPVESADPM
                     PPR"
     gene            12934..16323
                     /locus_tag="PLS35_06985"
     CDS             12934..16323
                     /locus_tag="PLS35_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HNI18438.1"
                     /translation="MLVAQFCGAFNDNAWKFMVALLGIRAVTTTLAPGQDLEAASQTQ
                     TTLAMVVFTLPLVLTSFVAGFFADRLSKRTVIITMKAVEVLLMAGATLALLSHPTGGA
                     WALVMLACMGVHSALFSPAKYGILPELLPHAQLARGNSILELFTFLAILTGTTAGGFL
                     LAGAGNQPWLAPLALTILALIGFAAAWAVPPVPAARAAGGLFDTLRGALSALRADRLL
                     RLAITGAVFFWTIASLVVQNVLVYAKAVLGLSDALATVPSALISVGIGLGALVVGRLS
                     RSRVEYGLVPLGAAGVATFLLTLGWWVPAFTGTLVVMIALGMSSALIFIPINALVQWR
                     APHDRRGSVIALENICVFTGILLGSLSGGVLANVGLSTVGIFLATAGLTTIGTAWAMW
                     LMPETFLRLVLVLLTHTIYRLRIVDQTHVPVTGGALLVPNHISFIDGFLLIASVDRPI
                     RFVVDAHYVNLPFFKPFMRALNVIPIASDGGPRVILRALRDAGQALDHGDLVCIFPEG
                     QITRTGNLLPFRRGFERIVKGREVPVVPVHLDRVWGSIFSFVGGRFVTKWPERLPYPV
                     TVSFGAPVPAGTPAHELRRLVRELGETAWHWRKSSRRPLHAAFVRAMRRHPFRLAMAD
                     ATKPHVSSLQALIGAIALARALTPHWQGQPSVGLLLPPTVAGALTTVAAALAGRTSVN
                     LNYTVGKAGLESAVRQAQLHTIVTSRKFIEKAKLEVPDGPTILWLEDIGATIGSLQKI
                     FAAALAILAPLRLIESACGQTRRVTMDDLATIIFSSGSTGEPKGVMLTHFSIDANVQA
                     VSQVLPLDPEDRILGILPLFHSFGYLVFWYVTLNGAASVFHPSPLDVTAVGELCAKYR
                     LTFLVCTPTFLQLYQRRCTPEQFSTLRVVLTGAEKLPLRLCQSFQDRFGIGPIEGYGV
                     TECAPVIAVNCPDFRAAGFFQPASRRGTVGQPLPGVSVRIVDPETDAILPPGMPGMLL
                     VKGPNVMNGYLGREDLTAQAMRDGWYITGDIATLDDDGFLTITDRLSRFSKIGGEMVP
                     HRLVEDALQQASGEELQVCAVTGVPDERKGEQLAVLHTLAEERIPHILAKAQAAGLPN
                     LFLPSHSRCFKVEALPILGTGKLDLRALKRMAMERLNLSGT"
     gene            16386..17378
                     /locus_tag="PLS35_06990"
     CDS             16386..17378
                     /locus_tag="PLS35_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNI18439.1"
                     /translation="MTRPVTKTLIDCHVHLAALPDGDNGCYISPKTLRSPLFRFLLWK
                     HGLSPKHPREANQKYLADLLTELRASRHVHKAVLLGMDGAYDQNGRLNQANTDFLIGN
                     DYVLKTAQAHPDELLAGVSINPQRRDAIEEVHRCADAGAVLVKVLANAQQFDPANKTY
                     TPFYRALAERKLPFLSHVGYEFSLIGKDQSVGEPDRLRVALDEGVTVIAAHACSYGLI
                     LYEKFLPTLHDLVQRYPHFYADISALTLPNRLRMLLHLRNYPQVHERLLFGTDYPLPV
                     FHLAAWGRVAMGRLRQLILTKNRFDRQVDVCQGLQVGFRSIGDLIPHASGQTPH"
     gene            17390..17887
                     /locus_tag="PLS35_06995"
     CDS             17390..17887
                     /locus_tag="PLS35_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI18440.1"
                     /translation="MTRDEILTHLRERILTFATSRLQKDVAEDLTQEVLMLLHEKYVR
                     VTELTELVPLAFQVLRFKMLDAHRKSLRRGEYNDHSVDDLPSADPGTDPASQLDQKQR
                     VDRLMAALGQLGDRCRQLFKWKLEGNSFPEIQTLMGQASINTIYTWDLRCRKQLLSLM
                     GGTWE"
     gene            17936..19243
                     /locus_tag="PLS35_07000"
     CDS             17936..19243
                     /locus_tag="PLS35_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18441.1"
                     /translation="MADHELEHLLGGFAANTLTPEERKQLYAAALHDQQLFTALADEQ
                     ALKELLANPDVRRRLLQALQHTSPTATGGTDTWWGWLKKPAGLAWAGGLAAAVFAIAF
                     GTRIYQESLDRARQSIVTEEVKPAAPPMPRVQPSMPAPQSAATPDPQAMEPADAAKEN
                     RRTDTRAKREQPLAGRASEQSAAKSSTDTAPPRRERDESSNNAPMAAAEKKSTGTPPS
                     ADRTLAAPAPPSTAPQPSAAPPLLQAPAGFSAAGAPAPKKSARALFYGEQSSPSLGQA
                     KPLGLRYSFVTRGTDGRTQEITAAMVARSSDPVRLTVEATQESYVQLLQNLGSAGTRL
                     WWPPQETGKISLKLQPGKRTDIPIPPPAENGLVTLIIRLSSKPFGPLTMQEVGMLDRF
                     SASLLIEAVSPGTTAGVQEQATYVVSQDPSTSAQLAVEIPVVQ"
     gene            complement(19268..19630)
                     /locus_tag="PLS35_07005"
     CDS             complement(19268..19630)
                     /locus_tag="PLS35_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18442.1"
                     /translation="MLELLLVLASLVVIGLTGLLTVVATPPRMVELGLWALALGLLIG
                     IPTGWWYHVVLYRALSARMALPRRWWQRPVELHPLLQPDEFRRVRPWFVAGALGFVLC
                     FTGGVAAIAGMLVMRFYP"
     gene            complement(19679..20311)
                     /locus_tag="PLS35_07010"
     CDS             complement(19679..20311)
                     /locus_tag="PLS35_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18443.1"
                     /translation="MPIQVIVLIGFLSLLPGCSLEQELSRTPRTAVEQVLLTQAVNQA
                     LTNLTVQLPVGVNVELDATGLESDRSRLRMTNSDLGSLNSPNRDILYIRDTVAAELGR
                     RGYLVRARDTESPYLVRVMAESFGTMQGITFFGMPPVQSVLIPFALPELTLYKQQNQS
                     GYARLHLDIYDNRTGEFLGSSATLVGRTFYNQYTMLFFLTWNRTDIDAPP"
     gene            20457..>22500
                     /locus_tag="PLS35_07015"
     CDS             20457..>22500
                     /locus_tag="PLS35_07015"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HNI18444.1"
                     /translation="MEQQHANPEPDPSASRQDPRYYQIASLGTLLIYGLCWLHFDLSI
                     RQIVVTIGTALLTQYVGTILVNLPAFDAKSALISSLSLCLLLRTDDLAVAALAAFLAI
                     GSKFVLRWHDKHLFNPTNLALTVMLGSGLGWISPGQWGQTAWLAFLIACLGSLVVTRA
                     ARADVTLAFLACYVGLLFARAVRLGDPLTIPLHQIESGTLLIFAFFMISDPKTTPDSR
                     IGRMVYALLVSFTALYVQFGLFKPNGPLWGLIACAPLVPVIDHVLPGSRYQWAHASTG
                     GSPHPAPIPVRTLSTVLSSTKEVSMRRAIIPSVLFTLSLLAWGGDALAFCGFYVGKAD
                     TKLFNKASEVAIARHDDKTVITMANDFKGEVKEFALVVPVPTVLEKEQIHVGDPALLK
                     HLADYSAPRLVEYFDQNPCLRYELMERRSMDAMKSVAPSAAPPERDKALGVTVEAQYT
                     VGEYDILILSGKESSGLEIWLTENGYRIPQGASAVLQSYLKQGMKFFVAKVNLGEQAK
                     LGLTYLRPLQIAFESPKFMLPIRLGTVNADGAQELFLYFLTKQGRVETTNYRTVRLPE
                     AQEVPLFVKEKFGDFYRDLFSQQVKRESERGIFMEYAWDMNWCDPCAADPLSAEELRS
                     LGVFWQESSGRMGKSLPMAQNVFLTRLHVRYDAAHFPEDLMFQETSDRGNYQARYIL"
BASE COUNT         4400 a         7248 c         6324 g         4528 t
ORIGIN      
        1 cagggtaata acctaggtta tggacggatt ttccctgtag tggaaaggag gatagatcca
       61 cctgattcac gcagaaccgt cgctcgatcc gcctgttccg ggatgtccgg tagccgacgc
      121 tctcagattt attccacgtt tgtggtaggc tggggcgcat cgtctgaaac gcgacgtttt
      181 gatttctcta tgactgacgc cgagacctcc ccgaccgttc tgagctcacg cgagatccaa
      241 ctccacgtgc tggggctcgt gctgctgctc ggtgtgcttg ggctgtggta ctggttttcg
      301 accggagcgc gggttgtccc gaccctcatc gagctactca aggacgatga tccgtcgaca
      361 cgaattgtcg cggccgaaca attagggcaa gtcggaccgg cagcgcgatc cgccgttccg
      421 caactcctga gccaggccac gcaggacggc agtcagcatg ccaacaccac cgccgccacc
      481 gcgctgaagt ggatcgattt gacggcggct cgccgggtga tgacccattt cgtcccgaga
      541 ctacaagatc ccgatgtgca actacgccgc acagcctgcg cggtgatcgg cagtctgggg
      601 ccgattgcca aaccggcggt accggccctg ctggccgtca cgcatgatgc cgatgcactc
      661 atacgccgca atgcgctcat cgccctggcg agcatcggca ttccgtctct cccgatcgcg
      721 gccgccctcg tggacggcct ccgcgattct tcgtcgctgg tgcgccagac ggcggtcgcc
      781 caatttgcgt ttacggttcc cccttcgccg gatgctgtgg cggttctgac gccaatgctg
      841 aacgatccgg ataaaggcat cgccaccctc gcacgtacgg ccttggacaa accgagggcc
      901 ggggatgcag cccatatcga atcgttgggc atgatgctcg gacattcctc tgcacgggac
      961 tatgcgttgc accaactggc acaattgggc ccggccgccc atgatgcctt gcccccggtc
     1021 cttgcgctgc tggaagatga acagcctctg attcgctatc tggccgccga aagcatcggg
     1081 ggaatgggag ctgttgccaa acaagcgctg ccggccttgc aacgacacca ggattccgac
     1141 ctcgtagttc gcgccgctat cgccgaggcc gtgagcgcga tcgaagccgg ggcggtcgtc
     1201 aacgccgaac cgttgagcaa ggaactgcca cgttgaccga tatcccgcgg caaacatcgc
     1261 ctcgcctccg gatgatggcg cgattggggg tctggatcag cctcctcggc ggattgcttc
     1321 ttccctggat catcatgctg accgtggaga tcctggttcg cgacatcccc tatccacggg
     1381 cctggaggtc gttcaccttg cacctctttg cgcccggcta taattttttc ttcgtggggg
     1441 tgctgacggc gctgccgttc gtgatcctgg ccctgatcat cctgttccac ctggggaccg
     1501 ccccggcgaa caacgccttg attgcctacc ggcgggcact cggcctcttc tcagcgggac
     1561 tgagcatggc cctcatcgcc gcctgggtcc atgtcgacgt gctcatccat cccgatgcgc
     1621 agggagcgct cgcctatttc tacttgccga ttcttttgct gatgatgctg ccgttcggat
     1681 acgggcttgg acgggcattg gccaaaatct ttctgccccg actgtcgggg tgacgcgcac
     1741 gggctggaca gcatcgacag cgtgagtata atgtgggcca tgcgtcgcgc gatcgtacac
     1801 gggggccgat gattcgtctc ttaccctgcc tcatcctgct tgtcctctgc cattccacct
     1861 ctctggctgc ctcgaaatat gaggacagtg tcaaagaact cgccgaaggg gtggcggctg
     1921 aggccatcaa aatgaaaaag tcgcggctcg cactgctgga ctttgtggac agcaagggag
     1981 acgtgacacc catcgggcaa ttcctggccg aagagctggc aactcatctg cttgtggccg
     2041 gagagctcaa agtggtcgat cgcaagttgc tgatcgcgac catggaaaag caccagctga
     2101 cccacctcga tacgtcacaa gccaaggccg ccaagaaagt cgccaaagcc ctgcgcgcag
     2161 acctctacgt caccggcgcg tatctggaga ttcccgaagg cattcaggtc accgccaagc
     2221 tgattgggcc ctatacggtc taccccgtcg gcgcctcacg gaccatcgta ccgaaatccg
     2281 gccccttggc agccctgctg aaacaggcag ccgctccggc tccggccacg gcggccgtgg
     2341 taacggatag tgcgccggtc ctcagcgctc acgaaaatga cttgtacaaa ttcacgattc
     2401 tcagcgtgac acggcaggac gacctcatcg gtctcgatct cgtcttcacc aaccgtacgg
     2461 cgcacccggt caagatcggc tgccagcttc tcgacaccta tcttgtggac gagcatggcg
     2521 agcagtggaa acagaatgtc gcgcagaatc gtgaaggcct ctgtacgcgc gggatggagc
     2581 tgaaccccaa gcggcagcag cgtgcgagcc tctcgttcct ggccaccaat aagccgccga
     2641 caggcccgat cacgttgcac taccatgaaa cgtcgcccag ggctgatcgg atcatcaccc
     2701 tggacggatt gaaaattgcc tccgcgccgg tcgttgagcc aggcgccccg gccgcgcagg
     2761 aatcaacgcc ccccacaccg taggtccacg tctcattact tgcacaggaa cttacccatg
     2821 attaaacaag tcttgtcgat agctggttct gattcaggcg gtggcgcagg cattcaggca
     2881 gatctgaagg ccatgtccgc caatggcgtc tacgccatgt cggtcatcac ggccatcacc
     2941 gcacagaaca cagaggaagt cgccgacgtg ttcgaactgc ccacatcaat catcgccgct
     3001 caactcgatg ctatcttcga cgactttgac gtggcggcgg tgaagaccgg catgctctcc
     3061 tccgcagaga ttatccggac ggtcgtgaaa ctcctgaagc ctcagcaggt gaaacatctc
     3121 gtggtggacc cggtcatgat tgcgaaaggc gggcaggcgc tgttgaagcc ggatgccatc
     3181 gataccctca agagcgcgct ctttccgctg gctttgctcg tcacgccgaa cgtgcatgaa
     3241 gcgcagcagc tttcggggat cgagatcact tcgttggccg atgcccgccg tgccgccaaa
     3301 gtcattcatc agttcggttg cgccaatgtc ctcatcaaag ggggccatct gcaaaaagag
     3361 cgcgggaccg atctgctcta tgacggacgg ttcttcaaca tcttcaaggg tgaatacatc
     3421 gacacgcccc atacgcacgg caccggatgt acctttgcct ctgccatcgc ggcgcatctc
     3481 gcccgaggca aaagtgtctc tgatgccgtc caaaccgcca agacctatct cactgaagcc
     3541 atcagacatg gcctggccat tgggcatggc aagggaccga ccaatcattt ttacttcttg
     3601 aatcagggtt agaggctatg agtcgagggc ttggatcgcc actgtcgttt ctgctcgccg
     3661 gatggggctg ggtgctgctg accgcactgt tgggactcgc cgcatttggg ggcatcgtac
     3721 ggggaggccc gcttccctcc ggccttcgcc ttttacacgt ccacggagtg ctgatcggcg
     3781 gggttctcca attgatgacc ggcctggcga tcgcggcaca tgatctgtcc ggccatgagg
     3841 ggaaacggac gacacaccgc ttgctctttc tcagcatgaa tcttacggcc gttgggtttg
     3901 ctgccggagc ctggttccgg aatcccacct tgaccctggc cgcaggattc ctgctggccg
     3961 cgctctttgt ccccatggcc cgccatatgc tgactgcgct ccggacctgc tcaggatgga
     4021 caccattggt acatgtctat ttcggcatca gcttcgccgg cctgttcggc agcatggtgc
     4081 tgggaggcct gctcgccgac gcctggtatc ccacctggca cgggattctc cgtctgggcc
     4141 atctccatgc aggccttttg ctgttcctca tactcggagc cgtcggaacc atccaactga
     4201 cgctcccgat gatggtacat cgcccgctgc acagccccgg gatgggccag gcggtcatgc
     4261 tgcttctgcc ggcctccgcc gccgggctga tcacgggatt cgtgttgtcc tccgttcata
     4321 tccagttggc ggcgggaacc gtgctcctga tcgcgctggc gctgtgctgc atcaacctgc
     4381 tgcgcacatg gagtcaggcc ggtcaacccg gctctgccgc cacggaccat ctcctcacgg
     4441 cattgagctt cttgctgatt atgaccttca tcggcttggc attcgggatc aacgtgttat
     4501 cgacaccgcc ggccatgccc tatgggacgc tgcatctggt ggcctacacc cacaccgcct
     4561 tcctcggttt tctcctgcaa agcatggtcg gcggactctc cttcgcgttg ccgtcgctgc
     4621 tggccacatc gcggatcgcg aatcagaaaa agcggggaac ctatcgcgaa gaccttgaac
     4681 gcatcatgaa ccgttggcgg gcggcccagg ttgcgaccgt aagttttgga tctttggggt
     4741 tggtgctcgt cgcctcattg acctggaatc ttcccctgtc ctcgccctgg ctgcaaacga
     4801 gcacctggat aagcttggga ctgttgctgt ttcacttcac catggtcacc gtcaaactcg
     4861 cacaggcgac cggcgctcaa ccgtcccatg aaggagcggc gaaataaccg ccatcacttc
     4921 accactatgg aagaagcgct ttcatttcac gatcaggccg gccaccggat tgcggcggtg
     4981 ctggccacac caccacagag caccgactct gtcgcggtgc tctgccatgg ctttttatct
     5041 cacaagaaca gcacgagtaa ccaagccctg gctgaactct tgaaaacacg cggcatcgcc
     5101 acgttgcgat tcgactgttt cggccatggt gagagcgatg ggcccttcgc caagctgacg
     5161 accagcatcg gggtgactca ggcacgcgct gcattaacct atgccatgac ccggggttat
     5221 cggcgactcg cactcgtggg ctcgagtttc ggcggccttg tctcgctgct cgcggcttct
     5281 gaatggaccg atctacataa ctccgcgccg gaggatgttc cccctctcgc ctgcctcgca
     5341 cttaaatgcc cggtcgttga tttcggagaa gagctgcgcc tagagctggg ggaagatggg
     5401 ctgcaggagt ggcaacgtac aaacacaatc ccggatcttc atggaggtcc ttcccgcctc
     5461 cccctcgact atgccttcta ccaggattgc ctcaaccgca tcgcctacga accggctcgt
     5521 gccattgcag caccaaccct gatcattcaa ggggatcatg acgaatacgt gcctctgcac
     5581 caaagccaac gactgtatgc atcgctggca ggccccaagc acctggaaat ccttcccggt
     5641 gctgatcacc ggtttacaaa cccttccgac tttcagctga tgctgacatt gctcacggat
     5701 tggattgcgg cgcctcccgc ggcgtgaccc tctctcattc cccgagcact acacaatgaa
     5761 gcggccgagt cgcctggccg cctgcttcac agacgctccc gcgaacaaga tcgaaggcat
     5821 tttcctgcac ccactgctcc aacttgcaca gcaaaaaata acgaacacta tcgcacagcg
     5881 ccattctctg caaaatggct tgtgacgcgg ctttctgacg ttcgcacgaa ttttcgagct
     5941 ggcatgccgg ttgctcatcc agcgtctgga atttcggagg aactatcatg aagacgccca
     6001 agagtgtgtt ggttatcggt gttgccggtt gcttatcttc cgcgctgtgt tcaacagcct
     6061 tgcccccaac cgccgctgcc gcagacacca cgctcagcct gctgcagcag tcatcgactg
     6121 acgcggtggc aatcatggcc cagaacagcg gcacggctcc catatttacc agtccaactg
     6181 ccctcgtcct cagcgcccag aagggacaaa cggcggtcgg caccctcaac ctgaaaaaat
     6241 ccagcacgga tcaacatagt tattatctca gcacgaacca ggggtgggtc tggatgaacc
     6301 cgccctatgg cagcacgcaa accattacga cggaaaccga tcagctggtg atcacggccc
     6361 aaacggccaa tcttgcggcc ggaacccact ccgccgtggt gtacatcgtc gattcaggcc
     6421 cgaacaactt caccaacatg ctccgcatcc cagtcaccct caccgtcacg gcgacggcag
     6481 tgacaccacc gccgcctccg cctcctgcgg ctgtcccacc ggccattatc ccgacgccca
     6541 gcccggtcgc cgtgcctcct cctccggctc cgactcctgt cgtggcgccg gtggtaaccg
     6601 gaacggccat cacgtcgagc ccggctgcgt tggcgttgag tgccgtcaag gggcaaaccg
     6661 ccgttggaac cctctcactg cggaagggcg gtaccgatca acacagctat tatctcagca
     6721 cgaaccaggg gtgggtctgg atgaatccgc cctatggcag cacgcaaacc attacgacgg
     6781 aaaccgatca gctggtgatc acggcccaaa cggccaatct tgcggccgga acctactcgg
     6841 ccgtcgtgta tgtcgtccaa tccggaccga ataacttcca gaacatgctc cgcatcccgg
     6901 tcaccctcac ggtgacggca tcggccgtgg caccgccgcc tccgcccccc gcggcagttc
     6961 ccccggccat cgttccggca ccgaaaccag tcgttctggc accgcccccg ccgccggctc
     7021 ccgcggtcgt accaccgccc ccggctccgg ccccacctgc gccagcaccg gtcacggttg
     7081 ccacggggcc gattcaggtg accccgtcct ccttgagcct gacgagcgcc aataacgtcg
     7141 gcaccttgac gctccgcaaa accggaaccg accaacacgc gtactatctc agcacgaacc
     7201 aggggtggat ctggatgaat ccgccctatg gcagcacgca gaccattacg accgaaagcg
     7261 atcaactggt catcaccgct caaacggctg gcctggcggc aggcacctat tcgggagtcg
     7321 tgtacatcgt tgaatctggt cccaataact tcgccaatac gctccgcatt ccagtgacct
     7381 ttacggtcgc cgcgggtcaa acagcctcca gcaccccgac gacaccgaca caaccaacgg
     7441 tggcctctcc gcccccgccg cctccgagcc ctccgtcacc agtccctgtg accacgacgc
     7501 cgaccccgac gtcgtccgcc tcaagcaccg cgcccaagac tgccagcgcg acggtaagct
     7561 ggaacgccaa tacggagtct gacctggccg gctaccgcat ctatgtggga acgaaatcag
     7621 gaagttacgg agtcgtagga ccattcgagg tgacaaacgg caccagcttc accgtaccga
     7681 acctgcccct cggagccacc tacttctttg ctgtctcggc ttttgacaag tcgggaaacg
     7741 agagtgcgaa atctgcggaa gtcagtaaga gtctgttcta gaccgaacga gaaaccgtga
     7801 gctggggcgc atgggcgccc ggctcacggc gaagctagca ccccaccgtc tctggcacat
     7861 cgaaatccgg tgtaactgtt gcgagtctcc gggggcaact tgaaccggcc ataggtcagg
     7921 agatatttcg gcaggtcgga ccaggatcct cccctcacca cgcgaaatgc tccggcatca
     7981 ggtccggaag ggttctgacg cggaccgttc tcgtaatagt gtgccccgta ccaatccgcc
     8041 acccattccc cggcatttcc tgccatctga tacaggccga aggggcttct cccttgttcg
     8101 tagcgctcta cgggagccag cacctggctg tagctgaacc tggcccctaa tccgaaattc
     8161 gccgtttcct cttgcgggct gtgattgccc cagggaaata accgtccatc ggtccctcgt
     8221 gccgcttttt cccactccgc ttccgtcgga agacgtgtgc ctcgccatcg acaataggca
     8281 tccgcatcga accagctgac gcctatgacc ggacggtcgc cgtgctgtga cagatcgagt
     8341 gactcccatt gccagggagc cggtcgcttc gtggaggcga gaaattcggc gtaatgcccg
     8401 gtcgtcacct catgacggtc gatctcaaac cggctgagcc acacgtcatg ttgtggcttc
     8461 tcatcctcca aggcgtccgt tccatcggca cccatccgga agactccttc ggggatcatg
     8521 atcatgggta cgtcggagtg gacctgcagt tccgaactcg ggcgcggctt acgcaactgt
     8581 tcaagaatcc cggcagtaga ttcagcgggg tacaacaggg ccatagccaa caggcagccg
     8641 agcaacgcag agtgcacgca gcgcagggtc gcaatgaaga tggatctggc tggacaaaca
     8701 ccgctcatag aggcgtgcac gtgagccctc gcggccagct cttgtgtcga acaggcagtc
     8761 aggggatgat tccgcccact acccagtgac gatcgtcagg cacatcgatg aggaggcgcg
     8821 ataaattcat ttccgcaagt tgcgccgcca cgtcgtcttc ggtgaaggcc gcgagcaatg
     8881 agtgataaaa atcccgcttc agaatcggat cttcatcggc agcatactgt tctaccaacg
     8941 cctgggcgga ttcaggagag tcaggccgca atagatccat gaccaaaatg cacgcgcccg
     9001 gctttgccag ctgcttcagc cgaaaccaga actgaagcgg gttcggcaca tggtgcagaa
     9061 ggctgttgga caggagcgcg tcggcctgtt ccggcaacac gagcgactga aaccgctcgc
     9121 aacgaagggt gatgtgatcc gtcaaccctg ctccggcaat ggcgtccgcc gccagatcga
     9181 gcatgggacg gctcgcatcg acggcggtca ctcgcgcatg tggataggcg cggagaaacc
     9241 gaatcgggat atcgccaggc ccgcatccga gatcaaccac atggccgccg tcccaggcgg
     9301 gaaaatattc acgaaagcgg tcgacaaagc cctggttctc ctcggcaaaa tccgccttgg
     9361 cataggcgag agcttgcgcc acatcatcca tcaattccgg ttcaagtact cgcttcatca
     9421 taagttccta catgtggatc aagatcactc accgctcccc aggacaggcg tcgactacca
     9481 caacatcccc cggcttcacc acgccctcat tgagcactcg cgcgtagagg cggctccatc
     9541 cgggatgcac ctcctgcgcg attcgacgaa aatcttcatc cttgaaccaa ggggcattca
     9601 agcggcacgg cgtggtgtaa ctggtcaatt caagccggac atgctcgccg atggtgagtt
     9661 ggtcacccgg cgccatgtgc tgccaatcga gaccggctac cgtgaggttt tcaccggacg
     9721 agcccggccc aatcgtatgc ccctcagcgc gtaacgcttc gatgcgttcc agcgagaaca
     9781 ggcagacggc ccgatctttt cctccgtgat acttccgatt gcgctgccgg tcgccggcca
     9841 gtccgtcgat ggtgatcctc gccgagggga cggccagctt cggcactcca ccatcggaaa
     9901 cgctgatctg atgcacatat ggagtcgccg atcgcgtcat ggccgcatca ggaacagaga
     9961 ccgccctcgc acgtttccat cgcggtcgcc tccaatgcga gccatccgtc tcgctcccga
    10021 acggccttca tatagactcc gcgagtggca taggccgctt caatttccga ccgctgctcg
    10081 gccaacagtc ccgacaagaa gaggcgggcg ccgcggacgg catacgcgca gaggctatct
    10141 gcggatgtca ggagcgtctg ccgatccaga ttggcaagca ccagggtggg atcgaacgca
    10201 tcatgggctt gcggggcctg agcctgtccc acggccagcg tgagacgcgt gtcaaacccg
    10261 ttcacctgcg cgtactcgcg agcacagtcc accgcgacgg catcgaaatc caccccgagc
    10321 gcctccgtgg ctccgagccg taaggccacc atcgccagga tgccgctccc ggtccccaca
    10381 tccaacacgc gatcgtccgg tcgcacagcc tgttgcagcc attcaatcaa cagctgtgtc
    10441 gtcgcatgat ggccggtccc gaatgcctgc ttaggatcga tgatcaattc gatctcatcg
    10501 gaattgagcg agacggttct ccagctcggc ctgatcagga cccgcccgat cttgatgggt
    10561 tccaccagcc gggaccattg tgcattccaa tcctgattgg ccaaatgatt gatggtcacc
    10621 gcgtgatccg ggtccggatg gccaagttga gtcagcgcac gtttcagccc ctgccaggta
    10681 tccggcccgc aacggcccgc gggccaatac agatgcacct cgtcaccctc ttgccaggcc
    10741 cccgtcacgt caggatcgtt gagcattccc aatagctcac cggcatccag cgaggtacgc
    10801 acatcaactt gtatccaatc ccctgtttgc accgcggcgg aatcggagga agaagattga
    10861 tctaccttgg aggttgacgt catctcagac tcccagtatc gtcagcatta ttgtgaaccc
    10921 agttacgtca gcagaattga agccatcccg tgagcagcat ctcgtccatg cactccggcg
    10981 agcggttcat ctggaaacga atggtcgccg cgtcagtgcg cggtttacca cggctcgtct
    11041 cacgattttt gccgcgaccc tcctcggcag catcattccg cataagttag gctggacgct
    11101 cttcggcaat ctggtcctcg cgtccggctt cgtcctgttc ctgatcgtgg cctggtatca
    11161 taacaggctc gaagatcgct tgcatcgact ccggcgctgg caaggcatca aacgagtgca
    11221 tctggcacgc ctcagcttac agtgggcgga gattccgctt cgcgaggtga ccgtgccgga
    11281 acggcatgga tatgccaggg atctcgacct taccggtccc cgctctctgc tgcacctgat
    11341 cgaccacagc atttcctctc agggtcggca acgtctcgcc tcgtggttct tggatcagcc
    11401 tcctcttgcc gcaccatggt cgagccggca ggccctcatc cgggaattga cgcccttgtc
    11461 gctgctccgt gaccgactgg tcctggaggc acaggcggtg gaggaagccg acatcgacgg
    11521 gcgccggctg cttgcggtgt tacagaaacg ggtggacgat tcgggactca tgccgatgtt
    11581 gctgacggaa tcggctctag ccgtcaccac ggccctcctg ctgctcgccg acttgatttc
    11641 cggccttggt aactattgga tgctgtcgtt cgggctgtac gccttcctgt tcctgtcggt
    11701 ccgcagccgg tcggaagaaa tcttcgacca tgcccagtca ttgcacctac aattggagcg
    11761 gctcagtgcc gtgctacagt atctcgaacg gcgctcctac cgaagcaccc cgcatctggc
    11821 caagctctgc gctccattga tgcaacaaga cagcagtccg tcgcgttcac tgaaacaggc
    11881 cgcttccctg atgaaccggc tcagtgtacg tgcccatccc ttggtccact atctcatcaa
    11941 tgccgtcggt ccgtgggatc tcttccatac ctatcgcctg caacaactcc aggaccggat
    12001 cgcctctgtc gcaccactct ggttcgagtt gctcgctgaa ttggatgccg cctcgtccct
    12061 ggctaccttc gcccatctgc acccgaacta tctctggccc acgctgcatc acgccggggc
    12121 agacggtcag gacaacggcg acgcgccgga actcgcggcc gaagggctgg cgcatccact
    12181 cattcctgcc gcggtccgcg tggccaacca ggttacgttg caagggcggg gccgaatctt
    12241 tctggtcacc ggctccaaca tgtccggcaa gagcacgttt ctgagaacca tcggcatcaa
    12301 cacctgcctc gcgcaggcgg gagctcccgt gtgtgccacg gcttttcgct ggtcgctggt
    12361 acgtatcggc agttgcatcc gcgtggatga ttctctcgac ggcggccttt cgttttttta
    12421 tgccgaagtg aaacgcctga aaaccatcct cgacagcacg agagacagac aaggacctcc
    12481 agtgctgtgg ttgatcgacg aagtgttcaa aggcaccaac aatcgggagc gactcatcgg
    12541 ggggaaggcc ttgattaccg cgcttgccgg aggcaatgga ttcggactgg tcacgaccca
    12601 cgatctggaa ctggctgagt tggagaagca gttaccgact gtcaccaatg ttcattttca
    12661 ggaaaccgtc gtggccggcg ccctgcactt cgactatcgt ctcaagccgg gtccctgccc
    12721 gacgaccaat gccttacgaa tcatggcact cgaagggctt ccggtggaga gcgccgatcc
    12781 aatgccgcca cggtaagtca ttgcatcgtt ccaggacgtt tcagcagact ggactcatga
    12841 ccgttgacag gcgcctgtgt ttcgccaggc aaggaggctc ctatcgatcc gcagtccgat
    12901 tcctcaccac cacccgcgcg gccgttgcgc ggattgctcg tcgcacagtt ttgcggagcc
    12961 ttcaacgaca acgcttggaa atttatggtc gccctcctgg gcatccgggc cgtcaccaca
    13021 acccttgcgc ctggacagga tcttgaagcc gcctcgcaaa cgcagaccac gctggccatg
    13081 gtggtgttta ccctgcccct ggtcctgacc tccttcgtgg cgggattttt tgccgaccgg
    13141 ctgagcaagc ggaccgtgat catcaccatg aaggccgtgg aagtgctgct catggccggt
    13201 gcaacgctgg ccctgctgtc tcatcccacc ggaggtgcct gggctctcgt catgcttgcc
    13261 tgcatgggcg tccatagcgc gctgttcagt ccggctaaat acggcattct tcccgaattg
    13321 ctgccccatg cccaactggc gcggggcaac agtattctgg agttgttcac cttcttggcg
    13381 attttgacgg gcaccactgc aggaggcttc ctgctggccg gcgccggaaa ccaaccctgg
    13441 cttgctccgc tggcgctgac catattggcg ctgatcggtt tcgccgcagc ctgggctgtg
    13501 ccgccagtgc cggccgcacg tgctgccggg ggactcttcg acaccttgcg cggcgccctc
    13561 tcggcattac gcgcggatcg gctgttgcgg ctggccatca cgggcgccgt gttcttctgg
    13621 acgattgcca gtctcgttgt gcagaacgtg ctggtctacg ccaaagccgt actgggcctt
    13681 tccgatgcac ttgccactgt tccttcggcc ctaatctcgg tcggcatcgg cttgggcgcc
    13741 ctcgtggtgg gacgactctc ccgctctcgc gtggaatacg ggctggttcc attgggggcg
    13801 gcgggcgtag cgaccttcct cctgacgctc ggctggtggg tgccggcgtt caccggtacc
    13861 ctcgtcgtga tgatcgcgtt gggcatgtcg agcgctctca tcttcattcc catcaatgcc
    13921 ctggtgcagt ggcgcgcacc gcacgaccgc cggggctcgg tcatcgcgct ggaaaatatc
    13981 tgcgtcttca ccggcattct gctgggctca ctcagcggcg gtgtgctagc gaatgtagga
    14041 ctttcgaccg tgggcatctt tctcgcgaca gccggcctca ctaccatcgg gacggcctgg
    14101 gccatgtggc tgatgcccga aaccttcctg cgcctcgtcc tggttctgct gacgcatacg
    14161 atctaccggt tgcgtatcgt cgaccagact cacgtaccag tcacgggtgg cgccctgctt
    14221 gtccccaatc acatttcatt catcgatgga tttttgctga tcgccagcgt ggaccggcct
    14281 atccgcttcg tcgtcgacgc gcactacgtg aaccttccct tcttcaaacc gttcatgcgg
    14341 gcgttgaacg tcattccgat tgcctccgat ggcggcccgc gcgtcattct ccgggccttg
    14401 cgcgatgcgg gccaggccct cgaccacggt gacctggtct gcatcttccc cgaaggtcag
    14461 attacacgga ccggcaactt actgccgttc cgccggggat tcgaacggat cgtgaaaggc
    14521 cgggaggtgc ccgtcgtccc ggtgcatctg gatcgcgtct gggggagcat cttcagtttt
    14581 gtcggaggac ggtttgtgac caaatggccg gaacggctac cctatccggt gaccgtctct
    14641 ttcggcgccc ccgtgcctgc cggcacaccg gcgcatgagc tccgccgact cgtcagggaa
    14701 ctcggcgaga cggcctggca ttggcgaaaa tcgtcccgac gcccgttgca tgccgccttt
    14761 gtccgcgcca tgcgccggca tccctttcgc ctcgccatgg ccgacgcgac caagccccat
    14821 gtgtcttccc tccaggcttt gatcggtgcg atcgccctgg cgcgcgcgct gacgccccac
    14881 tggcaaggac aaccgtccgt gggtttgctg ctgccgccga ctgtcgcggg ggcactgacc
    14941 acggtcgccg cggcactcgc cggtcgcacc agcgtcaacc tgaattacac cgtggggaag
    15001 gccggcctcg aatcggccgt gcgccaggca caactgcata ccatcgtcac gagccggaag
    15061 tttatcgaga aagccaagct cgaagtcccc gacgggccaa cgatcctgtg gttggaagac
    15121 atcggtgcta ccatcggcag cctgcaaaaa atattcgccg ccgccctggc aatcctcgct
    15181 ccgctccggc tcattgaatc ggcctgcggg caaacccggc gggtgaccat ggatgatctc
    15241 gctaccatca tcttcagtag cggcagcaca ggcgaaccca aaggtgtgat gctcacgcac
    15301 ttcagcatcg acgccaatgt gcaggccgtc tcccaagtgc ttcccctgga cccggaagat
    15361 cgcatcctcg gcattctccc gctattccac tcgttcggct acctcgtgtt ctggtatgtc
    15421 acgctcaacg gcgcggccag cgtgttccat ccttctcccc tcgatgtcac ggccgttgga
    15481 gaactgtgcg ccaaatatcg cctcacattt ctcgtctgta cgcccacgtt tttacagctc
    15541 taccagcgcc gctgtacgcc cgaacaattc agcacgcttc gagtagtgct gaccggcgcg
    15601 gaaaaattgc cgctgcgtct ctgccagtcg ttccaggacc ggttcggcat cggcccgatc
    15661 gaggggtacg gcgtgacgga gtgcgcgccc gtgattgccg tgaactgtcc ggatttcaga
    15721 gcggcaggat ttttccaacc ggcctcgcgg cgcgggaccg tcggccagcc gcttcccggc
    15781 gtgtccgttc gtatcgtcga tcccgagact gacgccatcc ttccgcccgg catgcccggc
    15841 atgttgctgg tcaaaggccc gaacgtcatg aacggctact tagggcgcga ggatctgaca
    15901 gctcaggcca tgcgcgacgg atggtatatt acaggcgaca tcgcgacgct ggacgatgac
    15961 ggcttcctga cgatcaccga tcggctgtca cgtttttcca agatcggcgg agaaatggtg
    16021 ccgcatcggt tggttgaaga cgcgctgcaa caggcctcag gggaagaatt acaagtctgc
    16081 gcagtcaccg gtgtgcccga cgagcgaaaa ggcgaacagc tggctgtcct ccatacgctc
    16141 gccgaagaac ggattccgca catcttggcg aaggcgcaag ccgccggcct tccgaatttg
    16201 tttctgccgt cccatagccg ttgcttcaag gtcgaagcgc ttccgattct tggaacgggc
    16261 aaattggatc tccgcgccct caagcggatg gccatggaac gactcaacct atcgggaacg
    16321 tgaaccatcc tcacggaggt tccggccagg tgacggccga ggaaccacca gtttttgatg
    16381 aacccatgac aagaccggtc acaaaaacgc tgatcgattg ccacgttcat ctcgcggcgc
    16441 tgccggacgg cgacaacgga tgctacattt ctccaaaaac gctacgcagc ccgctgtttc
    16501 gcttcctgct ctggaaacat ggcctctcgc cgaagcatcc gcgtgaagcg aaccagaaat
    16561 atctcgcgga tctcctgact gaattgagag cctcacgcca cgtgcacaag gccgtgctcc
    16621 tcggcatgga cggtgcgtat gatcagaacg gccggctcaa ccaggcgaac accgattttc
    16681 tcatcgggaa cgactatgtc ctgaaaacgg cccaggcgca tcccgatgaa ttgctggccg
    16741 gcgtatccat taacccccaa cggcgcgatg ccatcgagga agtgcatcgc tgcgcggatg
    16801 ccggtgctgt gttagtgaaa gtgctggcga atgcgcagca gttcgatccg gcgaacaaga
    16861 catacacccc cttttatcga gcgctggcgg aacggaagtt gccgtttctc agtcatgtcg
    16921 gctatgaatt cagcttgatc ggcaaggacc agtcagtggg agagcctgat cggcttcgcg
    16981 tcgcattgga tgagggcgtg acggtcattg cggcgcatgc gtgcagttac ggcctgatcc
    17041 tctatgagaa gtttcttccc accttgcatg atctggtgca gcgttatccg cacttttatg
    17101 cggatatttc cgccctcacc ttacccaacc gtttacggat gctgctgcac ctccggaact
    17161 atccgcaggt ccatgaacgt ctgctgttcg gaaccgatta tccgttgcct gtctttcacc
    17221 tggcggcatg gggacgggtc gcgatgggaa ggttgcgaca actgatcctg acgaagaacc
    17281 gcttcgatcg gcaggtggac gtgtgccaag gacttcaggt agggttccgg tcgatcgggg
    17341 atctcattcc ccatgcatcc ggacagacac ctcactgagc acgaccgccg tgacgcgaga
    17401 cgagattctc acccacctgc gcgaaaggat tctgaccttc gcgacatcac gccttcagaa
    17461 ggatgtggcc gaggatctca cgcaagaggt gctgatgctc ctgcatgaaa aatatgtccg
    17521 cgtgactgaa ttgaccgaac tggtgcccct ggcctttcaa gtgctgcgct tcaaaatgct
    17581 ggatgcccat cgaaaatcac tgcggcgggg cgagtataat gaccactccg tcgatgacct
    17641 gccgtcggcc gatcccggca ccgatccggc ctcgcaattg gatcagaagc agcgtgtcga
    17701 tcgcttgatg gctgctctgg gacagctcgg agaccgctgc cgccaactgt tcaaatggaa
    17761 gctggagggg aacagtttcc ctgaaattca aacgctcatg gggcaggcgt cgatcaatac
    17821 catttacacc tgggacctgc gttgccggaa acagttattg agtctgatgg gcgggacatg
    17881 ggaatgatga tgggtgctca ctccatcaga gatcgcgttg tgacaggtat cgagcatggc
    17941 tgatcacgaa ttggaacacc tcttaggcgg ctttgcagcg aataccctca cgccggagga
    18001 acgcaagcag ctctatgccg cggccttgca cgatcaacag ctcttcactg ccctggccga
    18061 tgaacaggcg ttgaaagagt tgctggcgaa tccggatgtt cgccggcggc tcctccaggc
    18121 attgcagcac acaagcccca ccgctaccgg cggaacagac acctggtggg gctggctgaa
    18181 gaaaccggca gggctcgcct gggcaggagg gttggcggct gcggtctttg ccatcgcatt
    18241 cgggaccaga atctatcaag agagcctcga ccgagcgaga caatccatcg tgactgagga
    18301 ggttaaaccg gccgcaccgc cgatgccacg tgtacaaccg tcgatgccgg cgccacagtc
    18361 tgccgctacg ccggacccgc aagccatgga acctgccgac gcggccaagg aaaacagacg
    18421 caccgacacg cgcgccaaac gcgagcagcc tcttgcgggc agggcgtcgg aacagtctgc
    18481 ggccaaatct tcgactgata cggctccccc tcgacgtgag cgcgatgaat ccagcaacaa
    18541 cgccccgatg gcggctgccg aaaagaaatc gaccggcacg cccccttcag ccgaccgaac
    18601 gcttgctgcc cccgcaccac cttcaacagc gcctcaacca tcggcagcgc cgcctctctt
    18661 gcaagcgccg gccggatttt cggcggctgg tgctccagcc cccaagaaga gcgcccgcgc
    18721 cctcttttat ggcgagcaat cgagccccag cctcggccag gcgaaaccgt tgggccttcg
    18781 ctacagcttt gtgacgaggg gaaccgatgg ccggactcag gaaatcacgg cagcgatggt
    18841 tgcgcgaagc agcgaccccg tccgtctcac cgtggaggcg acgcaggaat cgtacgtaca
    18901 gctgctccaa aaccttggct ctgccggcac caggctctgg tggccgccgc aggaaaccgg
    18961 caagatttcc ctcaaactcc agcctgggaa acggaccgac attcccatac cgcctcccgc
    19021 tgaaaacggg ctggtcacgc tcattatccg gctatcttcg aaacccttcg gaccgttgac
    19081 gatgcaggaa gtcggtatgc tggaccgatt ctccgcgtca ctgctcatcg aagctgtctc
    19141 tcccggcaca accgcaggcg ttcaggaaca ggcgacctac gttgtgagcc aggatccatc
    19201 caccagcgcc cagctcgccg tcgagatacc ggtcgttcag taatgtgagg attgccgact
    19261 gtcggcatca cgggtagaac cgcatcacca gcattccggc tatcgccgcc acgcccccgg
    19321 tgaagcacaa aacaaaaccc agtgcacccg ccacaaacca gggacgcacc cggcgaaatt
    19381 catccggctg cagtaacgga tgcaattcta ccggtctttg ccaccaccga cgcggcagcg
    19441 ccatgcgcgc ggacaaggct cgatagagca ccacgtgata ccaccacccc gttgggatac
    19501 cgatgagcaa gccgagggcc agcgcccaaa ggccgagctc aaccattcgg ggcggagtgg
    19561 cgacaaccgt caggaggcct gtcagcccga tcaccaccag gctcgccagc acgaggagca
    19621 gttcaagcat gggccggatt atactcgaag gattgcaagg aagggtggct ctcgcgtatc
    19681 acggcggcgc gtcgatatcg gtgcggttcc aggtgagaaa gaataacatc gtgtattggt
    19741 tgtaaaacgt tcgacccacg agagtggcgg aagaaccgag aaactcgccc gtgcgattgt
    19801 cgtaaatgtc cagatggagt ctggcatacc cgctttggtt ttgttgtttg tagagcgtca
    19861 attccggaag cgcgaatgga atcagcacac tctggaccgg cggcatgccg aagaacgtga
    19921 tcccctgcat tgtcccgaac gattccgcca tgacccgcac aagatagggc gactccgtgt
    19981 cgcgcgcccg caccagatac cctctgcgtc ccaattcagc cgcgaccgta tctctgatgt
    20041 acagaatgtc ccggttggga ctgttcaacg agcccagatc ggaattcgtc atgcgaagtc
    20101 gggaccggtc actctccaga ccggtcgcat cgagctcaac attcacaccg acaggcaatt
    20161 gcacggtaag attcgtgagc gcttgattca cagcctgcgt cagcagcact tgctcgacag
    20221 ccgtcctggg cgttctggac aactcttgtt cgagcgagca gccggggagc agagacaaaa
    20281 accctataag aacaataacc tggatgggca cggcatcacc tcggctgaac gatggattca
    20341 agttcatgct aatggattac gcccatctac tcaatggggg acatccctag agccaggcag
    20401 atggcccgct gaaaggattt tccgagtcat gccataccct ccgtaaagga ggagttatgg
    20461 agcagcagca cgccaatcct gaacctgacc cgtcagcatc tcggcaggat ccccgctact
    20521 accagatcgc cagcctgggc actctcctca tctatggtct gtgctggctc cacttcgatc
    20581 tgtcgatccg gcagattgtg gtgacgatcg gcaccgcatt gctcactcaa tatgtcggga
    20641 ccatcctcgt caaccttccg gcattcgatg ccaagagcgc actgatctct tcactgtcac
    20701 tgtgcctcct cctgcgtacc gatgatctcg cggtcgccgc attggcggcc ttcctcgcca
    20761 tcggcagcaa gtttgtcctt cgctggcatg acaagcacct cttcaatccg accaatctgg
    20821 ccttgaccgt catgctcggc agcgggctgg gatggatctc gccggggcaa tgggggcaga
    20881 ccgcctggct cgcctttctc atagcctgcc tcgggagtct ggtggtcacc cgcgccgcac
    20941 gcgccgatgt cacgctggcg ttcctcgcct gttatgtcgg gctcctgttc gcccgggcag
    21001 ttcgactcgg tgatcccctc acgattccac tccatcagat cgagagcggc acgctgctca
    21061 tctttgcctt cttcatgatc tcggacccga agaccacgcc ggattcacgc atcggacgta
    21121 tggtctacgc actcctcgtg tctttcaccg cgctctatgt gcaattcgga ctattcaagc
    21181 ccaacgggcc gctttgggga ctcattgcct gcgcgccgtt ggtgccggtt atcgatcatg
    21241 tactccccgg ctcgcgctac cagtgggcgc acgcttcaac cggtggctct cctcacccgg
    21301 ctccaatacc agtccgcact ctctccactg ttctgtcatc aaccaaggag gtttctatgc
    21361 gacgtgccat cattccgtca gtattattca ccctgagcct gctcgcctgg ggtggcgacg
    21421 ccttagcttt ctgcgggttc tatgttggta aggccgatac gaaactgttc aacaaggcct
    21481 ccgaggtggc catcgcgcgg cacgacgaca agaccgtgat cacgatggcc aatgacttca
    21541 agggcgaggt gaaggagttc gcgctggtgg tgccggtccc gactgttctg gagaaggagc
    21601 agatccatgt gggcgatccg gccctgctga aacatctagc ggattactcg gcgcctcggc
    21661 tggtggaata cttcgatcag aatccctgcc tccgctatga gctgatggaa cgccgcagca
    21721 tggacgccat gaagagcgtg gcaccgtcag ccgcgccacc tgaacgtgac aaggccctgg
    21781 gcgtaaccgt ggaggcgcag tatacggtcg gtgaatacga catcctcatt ctctcgggca
    21841 aggaaagcag cggcctggaa atctggttga ccgagaacgg ctaccgcatc ccacaaggcg
    21901 cctcggcagt cctgcagagt tatctcaaac agggcatgaa attcttcgtc gcgaaggtca
    21961 acctcggtga acaagccaag cttggcctca cgtatctccg gccgctgcag atcgcgttcg
    22021 aatcgcccaa gttcatgctg ccgatccggc tcggcacggt caacgccgac ggtgcgcagg
    22081 aattgttcct ctacttcctc accaagcaag gccgtgtcga aaccacaaac taccggacgg
    22141 tgcggttacc ggaggcgcag gaggttccac tgttcgtgaa ggaaaagttc ggcgacttct
    22201 accgcgacct cttcagccag caggtgaagc gggaaagcga acgcgggatc ttcatggaat
    22261 atgcctggga catgaactgg tgtgatccct gtgcggccga cccgctgtcg gctgaggaat
    22321 tgcgcagtct cggcgtgttt tggcaggaga gcagcggccg gatgggcaag agcctgccga
    22381 tggcccagaa cgtctttctc acgaggctgc acgtccgcta cgacgccgcg cattttcccg
    22441 aagatctgat gtttcaggaa acctcggatc gtggcaacta tcaggcgcgc tacatcctgc
//
LOCUS       DAOQEW010000045        22102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_573_length_22102_cov_7.782147, whole genome shotgun sequence.
ACCESSION   DAOQEW010000045 DAOQEW010000000
VERSION     DAOQEW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 22102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22102
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_22102_cov_7.782147"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLS35_07020"
     CDS             complement(<1..349)
                     /locus_tag="PLS35_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNI18445.1"
                     /translation="MVLTADAPFAFIGCSELQESIAHQADDEKELAELIEEVPLDSIH
                     FHTHSYFLRHGLIEGTYPNDFAQWVVMQIGDHVLGERLAVLDPFDYPDLENLREELIS
                     IIDDHLSRSPIIPR"
     gene            953..1720
                     /locus_tag="PLS35_07025"
     CDS             953..1720
                     /locus_tag="PLS35_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNI18446.1"
                     /translation="MDARVIAGASNPWSSVYMLNPYLLFWSIGLLFFVAEWLRPARKI
                     TYRAVFWRDLLALGAYNLSFLLVVQVTDRIPVPQYLPVALYNLPTAGKLLLFYIVEDF
                     GLYWVHRLMHTRVVWPIHRWHHSPTSLYWLAGIRTTIPHIALFNVTYVAALPLLHDAS
                     GWAFQVIMVEHIVRNNWMHVNVTWKSSWLEWVFVTPRYHHIHHSSDPAHQVKNLGALL
                     TIWDRLFGTYYNPDDVTGELKFGLSERVNPIRLVIGV"
     gene            complement(1858..3171)
                     /locus_tag="PLS35_07030"
     CDS             complement(1858..3171)
                     /locus_tag="PLS35_07030"
                     /inference="COORDINATES: protein motif:HMM:NF023301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase domain-containing
                     protein"
                     /protein_id="HNI18447.1"
                     /translation="MRIMTVSEILFSSRPHLLYTPAMRTRRMSLLLLSLAVPTLFSGC
                     ETTDKMLGAAERAVGSTTGRTVLDIVHGKDPADIARQRVEQYGRDPQALLRDLRAIQR
                     DFQALMAALTGEVGKKWGTKEVKLPEQKKYVKYTQNYRSRAIVDFDAGNILIETLDEK
                     DPRASLKNAVVTTLLTPNDPRSVDLFSDKEITLTGDKEPYLLGLVVNQAGKPVRTPTE
                     AEQFAESLLSKSTTRPVEQENGAKTAHLVNIPMVTNFSHKQAEKYRTVVAQFAERYQI
                     SPSLVFAIIRTESNFNPFAVSSAPAYGLMQLVPTSGGRDAYRKAKGEDKAPSRDYLFD
                     PDNNIELGTAYLNVLTYSQLDDVTDMVSREYCVISAYNTGAGNVFKTFAKDQRTALQQ
                     INGLQPSTLYDRLRSGLPYQETRDYLAKVVGFRKQFVVIGENGTQ"
     gene            3317..3919
                     /locus_tag="PLS35_07035"
     CDS             3317..3919
                     /locus_tag="PLS35_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI18448.1"
                     /translation="MNTRASAAVWCWVIVLLMTLAPSPGRAAQTPTEAVQVTMKDLLY
                     LLTELKETSRSAQRQWEIEQVVRRAFNYEEMAERALGEGSEKTTIADRRQFTRLFVQV
                     LRDDLADKLRDYSLPQVAYLSEQGDGTGTQVLVAPAGREVDTKIEFSVVERSGRWLIN
                     DVSIDGASIVATYQTQFSRILREGSFSDLMEFLKQKAVVA"
     gene            complement(4086..>4472)
                     /locus_tag="PLS35_07040"
     CDS             complement(4086..>4472)
                     /locus_tag="PLS35_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI18449.1"
                     /translation="DGIVLTIQPAVVLLATISHVCGLPDATTLYERRTRRRLHCTESS
                     IDDDSPPSLSSTSPDTALTGREREIVYLIGQALSNKDIAERLCISSVTVRHHLTSIFG
                     KFDVNSRQKLLLRAYEYGLLKVHAHH"
     assembly_gap    4473..4572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4590..4997)
                     /locus_tag="PLS35_07045"
     CDS             complement(4590..4997)
                     /locus_tag="PLS35_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18450.1"
                     /translation="MMLCPATHCGQMMETDVRAGYDPDSGLECLFCPRCGHRGMKART
                     GVQLLFTGQHEYVFSYGPSLSHLKIVLSTVAINLFRTQGMPPTQLAAHVADWALLMGQ
                     VCGTVRFSGDLILSSCYEYCRREATKSPAVSSL"
     gene            complement(5043..5360)
                     /locus_tag="PLS35_07050"
     CDS             complement(5043..5360)
                     /locus_tag="PLS35_07050"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNI18451.1"
                     /translation="MPERNAQAPAGDEPIRNISRGATSRSIPKRKDRLQPSPQAETTD
                     TAEDIRTRIEKLAYARYQQRGQQDGYDYEDWLEAERIVQGESAPQSGEGSGRSGTHAL
                     HSA"
     gene            complement(5418..5846)
                     /locus_tag="PLS35_07055"
     CDS             complement(5418..5846)
                     /locus_tag="PLS35_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18452.1"
                     /translation="MSPTMHPVKQAGRSRVMIVDSAMQFGLKLADCLATRGYHAVLVR
                     DLESTLPQLGEIQPAAILFSHDSGGSHCDTPGMDALRALNALCPQAPVLALSEPVHPV
                     LSGESRSGDTLSAKSASPPLEHPVEAVLLTTLGIPCVRLQ"
     gene            complement(5843..6148)
                     /locus_tag="PLS35_07060"
     CDS             complement(5843..6148)
                     /locus_tag="PLS35_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18453.1"
                     /translation="MTTLAEIEGLITRELTRRGACTLEALAQQLQTCTWNQVFMAVDI
                     LSRRGTLVLRPLPEFQYTVSLAGSGSSTLRHLIPVTAGKTSLGVAAVDQVMNKEGQR"
     gene            complement(6393..7082)
                     /locus_tag="PLS35_07065"
     CDS             complement(6393..7082)
                     /locus_tag="PLS35_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18454.1"
                     /translation="MTGPFLDQTQQMEPIVTKILVVDDHAVVRQGVKQILNEQFQGAV
                     IGEARNAEEMIDRIRKFTWDIVVLDVGMPGKSGLDALKDLKQVCPKLPVLVLSAYPED
                     QLARRMLKAGASGYLTKDSAPNELVQALKKILGGGKFVSASMAELLVANLNEEAGRPQ
                     HELLSDREYQVMCLIAVGKSLKEIADDLCVGISTINTYRARILEKMQFKNNTELTHYA
                     LENRLVNRLVS"
     gene            complement(7106..9349)
                     /locus_tag="PLS35_07070"
     CDS             complement(7106..9349)
                     /locus_tag="PLS35_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18455.1"
                     /translation="MTNRPIHVLLIEDNPGDIRLVRELFAQTGNGQFTLTHVGRLDAG
                     IRCLSESLIDVILLDLSLPDSQGIETLIRMHAAAHGIPIVLMTGLEDEELGLHLIQAG
                     AQDYLIKGQTSAPLLARALRYAVGRKRLEEELREQTRFLQSVLNSMADGVVMADETGT
                     FRVWNPAAEQILGSGPAKRTVGHWSEDFTIFRPDRVTPYPAEDIPLARAIRGESVTGS
                     TLFLKRRSQGNDLWLDVSARPLRDEMGLIKGGVVVFRDITEARRTDEALRDSENRFRA
                     IMDNSPALIFLKDLEGRYLQANRQFETVSHLANKDLVGKTDEEIFAPAQAAVFRENDR
                     KVLQADAPMQFEESALHDDGPHTSIVVKFPLRNAEGRCYALCGIATDITDKQRAEEIR
                     QQLAKDRLLLLESTGEGIYGLDRQGRCTFINSAASRMLGYSPDELLCQDMHERIHHSS
                     PDGGIYHRTRCHIHETLVDGRGRKVDDEVYWRKDRTSFPVEYSSFPVIEQGKVTGAVV
                     VFLDITDRKRAEQQLTASHAQLRKLTARLESVREEERILIAREIHDELGQALTGVKLE
                     LSLLQDQLPSVPPTVHSRLESISGLVDATIQSVRRIATDLRPIVLDQLGLIPAIEWQT
                     HEFQSRTGIECRLDIYLRSACLSQTASTAMFRIFQEILTNVVRHAKASVVKITLQEQG
                     GGLVLEVQDNGRGITETELADPQSLGLVGMRERALLLGGSITFAGNAGSGTIVRVRIP
                     LDQPAQE"
     gene            complement(9346..9810)
                     /locus_tag="PLS35_07075"
     CDS             complement(9346..9810)
                     /locus_tag="PLS35_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18456.1"
                     /translation="MVVPELVKPIEILLVEDNPGDVRLTIEALKEAKVINHLTVVKDG
                     MEALAFLRRQGSYTAAPRPHLIVLDLNLPRKDGREVLADIKADDSLKRIPVVVLTTSQ
                     DEQDVLKSYNLHANCYITKPVDLDQFVRVVRSIEDFWLGIVVLPVSHQPGAV"
     gene            complement(9842..11629)
                     /locus_tag="PLS35_07080"
     CDS             complement(9842..11629)
                     /locus_tag="PLS35_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18457.1"
                     /translation="MIALPTTTVQTTAFDSSSRWTERILPILLFLCFFIATALGLYAL
                     SFLRAMLVAERGDELAMNAATVAETVDRVLFERYGDIQLFANDGVIRDGSSEERTARL
                     QHYHQLYWYYSWLGITDADGTVIAATDMRPASGPPPLNRAAFDAVRRSGTIYLEPVVP
                     SAMPPQQKAVGFMAPIYGPRGEFRGVVASRVPIDNLRSIFEQEGRLRYGKDPYDWLLL
                     TRDGAIISEKQTSAESPKGSAILDIPSRSRAASDRNQSGFVEEVHHRQGHSVVTGYAS
                     TRGYRDFPGFDWIVLFRQTHDQVYAPVDRLLWTVGGIGLLVILPLTGFGILVSWKLGR
                     ERSELLKTRQDLEKSVAELGRSNADLQQFAYVASHDLQEPLRMVSSYTQLLAKRYRGQ
                     LDADADEFIAYAVDGATRMQKLIQDLLAYSRVSTGSQPFEPTPMGAVLSYAMDNLLSA
                     IKESQAVITHDRLPTVRGDAKQLAQLFQNLLSNAIKFHGDQPPRIHISAQRREDEWLF
                     SVRDEGIGIDPQFAGRIFVIFQRLHTRAEYPGTGIGLAICKKIVERHGGRMWVESETG
                     KGATFWFSIPDPPQQPSVTRSEGATTGVA"
     gene            11785..12201
                     /gene="arfB"
                     /locus_tag="PLS35_07085"
     CDS             11785..12201
                     /gene="arfB"
                     /locus_tag="PLS35_07085"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNI18458.1"
                     /translation="MLSISTHVSIPDEEIELTAVRAQGAGGQHVNKVSSAIHLRFDVR
                     ASSLPPFYKERVLELRDHRVSRDGVIIIKAQDSRSQERNRALALERLRELIQQAAIPR
                     IPRRPTKPTKGSKKRRLEDKSKRGQLKSLRSRPHEQ"
     gene            complement(12272..12589)
                     /locus_tag="PLS35_07090"
     CDS             complement(12272..12589)
                     /locus_tag="PLS35_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNI18459.1"
                     /translation="MGNKLYVGGLPYAATESQLSNLFSAHGTVESARVITDKFTGQSR
                     GFGFVEMSTAEEAKAAITALNGSDMDGRQLTVNEAKPQEPRSGGGGGGRFGGGDRGGR
                     NRF"
     gene            complement(12883..13752)
                     /locus_tag="PLS35_07095"
     CDS             complement(12883..13752)
                     /locus_tag="PLS35_07095"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNI18460.1"
                     /translation="MQQVSPTPVPAHIVHRVHHKFWTYLLFWSIVAGSVICVPGYGIL
                     YGYSWLDWTMFAVLYLITGLGITVGYHRLLTHRSFECPDWVKACLLVAGGWALQNSGA
                     KWTADHLRHHAHCDDPADPYNAKRGFWYSHCGWLLNPVPCNDERFGSRVMQDPVAMWQ
                     HRNYAAIVVVGLALPFVIGFLHNGWQGGIGGFMLAGVGRTFAVLNSTFCINSVCHIWG
                     EQPYGTSDSSRNSWLVSLLTFGEGYHNYHHTYPSDYRNGPLWYNFDPSKWLIYTLSKL
                     GLASSLRTASPGN"
     gene            13962..14312
                     /locus_tag="PLS35_07100"
     CDS             13962..14312
                     /locus_tag="PLS35_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNI18461.1"
                     /translation="MPTGYPRKATSMVLWSCVVIGLSGLYAPVNAAPDPASTGQEQPA
                     ATSEEPGSSGKRPEGQGRGGRPGRKACADDVKKLCAGVKAGEGRIMQCLREHTQELSP
                     ACADMMQQRGKHRP"
     gene            14476..14856
                     /locus_tag="PLS35_07105"
     CDS             14476..14856
                     /locus_tag="PLS35_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18462.1"
                     /translation="MSDTTRQEQFSVDLRQHPRLRVPAPFACSLSRLGLAKWLGRGGE
                     GIGVVFDVSMRGAKVMSEAGIQRGDRVSVSLSLPNQVSPMTVEKATVRWEREQVYGLE
                     FVDLSPVAEMRLRKFISLATKPAA"
     gene            15031..15969
                     /gene="lpxL"
                     /locus_tag="PLS35_07110"
     CDS             15031..15969
                     /gene="lpxL"
                     /locus_tag="PLS35_07110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HNI18463.1"
                     /translation="MSNPQANHKRRQPRSVYHPMYWPTWVGITLFRLLSWLPYHAQLA
                     FGRQCGRLLHGLLTKRRDIVRVNLALCFPEQSPEERNRVALHSFESMGMGVLEIAMAW
                     WAKDPRAHCECTIKGLEHIRDALHRGRGVLLCGAHLHAAELAGRFMALEQPVAIVYRP
                     QNDVVADMVANRCRSRYYSELIQHRDMRGILRALARNQVVWYAPDIDAGSKRSVFAPF
                     FGVPASSLTATSRLAKVSGAAVVPCFYYRRTDGSGYDIEVGEALDHFPSGEMIEDATR
                     VNRLIEGAVRRVPEQYFWQHRRFKSRPPGEPPLYRR"
     gene            16116..16493
                     /locus_tag="PLS35_07115"
     CDS             16116..16493
                     /locus_tag="PLS35_07115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18464.1"
                     /translation="MDQDKQVSPVRIELRQHRRCPVAETCLLSFAPFAPTLSFNGDAE
                     GEGTVLNLSPGGCKISSEAGVKVGDAMSLIILLPGETAPTAVDLALVRWGKDLSFGVE
                     FVSMGTAEQDRLRQFLASAAVSE"
     gene            16540..16872
                     /locus_tag="PLS35_07120"
     CDS             16540..16872
                     /locus_tag="PLS35_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18465.1"
                     /translation="MEETRTLRKSRRVAADCRLEFVGEDEIEGEAEVLDLSSTGCMAK
                     SDQAMPPGLRMKVSLLLPDGHEWPVRIEEAVVRWARGGEFGVEFLVMRPPQLQRIQQF
                     VIKHRPLG"
     gene            17065..17844
                     /locus_tag="PLS35_07125"
     CDS             17065..17844
                     /locus_tag="PLS35_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI18466.1"
                     /translation="MFTWVGRKTIAGYAYLAALATLFAQAVFDLIIPPKQGRGETFRV
                     LVRQILFTGVDALPVTTVIALLLGIIIVTQAGTQLPRLGAGGLVGSIIVVTVIRELGP
                     LVTAFIVVGRSGSAIATELGNMSVTREVVALRLMGIPISRFVIMPRMVGMVLSMVCLT
                     LYFDVVAVFGGYLVADAQLTIPLDAFAESVIRALSTTDVLMTAIKGLGFGSAVAAVCC
                     YHGLAVRSSYTEVPQQTTRAMINSFVLCLLIDVVVTVPLYL"
     gene            17841..18572
                     /locus_tag="PLS35_07130"
     CDS             17841..18572
                     /locus_tag="PLS35_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI18467.1"
                     /translation="MSEAAIIFDKIRAPMLGGDLWLELDLKIMAGECVVCVGPSRAGK
                     SLMVELAAGLIAPESGRVLVLGQGWSEVSDDAPGSVRLKVGVVLQQPGLLSNMTLFNN
                     VLLPLRYHRSAMPDRHREQVVMGELERLGLASLRDRFPAELNQGEARRGAIARALILE
                     PEVLLLDDPVAGLDADMVLGLKSSIDERRAQRPLTVLAALRTCSPFVDAADRLIVLRD
                     GHVEADGTRDTVRDLAPEALRRYVE"
     gene            18605..19597
                     /locus_tag="PLS35_07135"
     CDS             18605..19597
                     /locus_tag="PLS35_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNI18468.1"
                     /translation="MMHYSHRLSSGRLAQIVGTFVLIPAVILAVAGFWMSRTEHLFES
                     KYLLKASLSKSYGLEPGAPIVVSGIRIGRVKQVDLNDRGTVDLTLQLLARYQDMVKEN
                     SELHITKSGVVVGQTQVDIAMGTASSPVLSEGSIIRAVEPRDIGDLLNEVEPVLMAVK
                     QTLLRVETITQDMQGGLKAGGKALEQVALATQELPAVVASVQRTIASVEQTATALPAM
                     TGSIKRTLSMVDRVTADVQQTTGRLPAILDSAQTTLTSVKRLSDSVSEVSQELGPIIH
                     SAETTLADMSILVRGAKQTFPFNRFVQNAGPAPKESPPPSREGTQSLRGDQLRR"
     gene            19756..20553
                     /locus_tag="PLS35_07140"
     CDS             19756..20553
                     /locus_tag="PLS35_07140"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18469.1"
                     /translation="MRGDLTRAAEEYEAARRMAESLDDTQGLIVAINDVGRVALRRGD
                     VPRAVSSHERAVALAQQRGEPDLLIASLTDLATAEHQSGALQEAEHRYEQALDLAQQR
                     GDRSAEATIRNNLGLIRQTKGDVEQAAQLFRQALALNQALGNLESEASNHVNLGMLAE
                     ARHEYDAAEREYERALELDKTAEQRMEIAADLLRLGKIADRRGFPDRGQTYSERAYQS
                     YLAQDAFAEARSALSQAVACAKKLERAGDIARLERELTRLTALHPAR"
     gene            complement(20582..>22102)
                     /locus_tag="PLS35_07145"
     CDS             complement(20582..>22102)
                     /locus_tag="PLS35_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNI18470.1"
                     /translation="LKNLNVDYLKKNHWMPLKRDRAAIEILTDDPGDLDRVQDIKRTF
                     PGLNIRFAVSLRRDISLFLASTTGTPDVTNKMNENVSDILGELVNESQLEAQEEASAA
                     GLDENDNAIVRLANQIIADAFRQGTSDIHIEPYGEKRDTLVRFRVDGDCFEYMKIPPS
                     YRRAIVSRLKIMASLDIAERRKPQDGKIKFKIGENKEIELRVATIPTAGYNEDVVMRI
                     LAASEPLPVDKMGFSERNLREIKSIAEKPYGIILCVGPTGSGKTTTLHSVLGYINTPD
                     IKIWTAEDPVEITQYGLRQVQVHAKIGFTFAAAMRAFLRADPDVIMVGEMRDKETADT
                     GIEASLTGHLVLSTLHTNSAVETITRLLDMGCDSFSFADAMLGVLAQRLARRICKDCK
                     EAYQPSKEEYEELRLGYGPDYWDALKVPFDANFRLYRGKGCDACNRTGLKGRVALHEL
                     LLGTDKMKKLIQNKAKTDEMLHAGMEDGMTTLVQDGIQKVLQGHTTYKEVKAVAIK"
BASE COUNT         4294 a         6113 c         6758 g         4837 t
ORIGIN      
        1 ccctaggaat gatggggctg cgggaaaggt ggtcgtcgat gatagaaatc agttcctctc
       61 gcaagttttc caaatccggg tagtcgaacg gatcaagaac cgcaagacgc tcgcccagca
      121 cgtgatcccc gatttgcatg acgacccatt gcgcaaagtc attgggatag gtgccctcaa
      181 tcagtccatg gcggaggaaa tagctgtgtg tgtggaaatg gatggagtcc agcggcactt
      241 cttcgatgag ctccgccagt tccttttcat cgtccgcctg atgcgcgata ctttcctgga
      301 gttcgctgca gccgataaag gcaaacgggg catctgctgt aaggaccaca atccagactc
      361 cttcagagag ttcagccgta agtcacacgc atcccgcgtc tctcggacaa catctaatcc
      421 tcaggatgcg tccctgatgg gttcactata aaacttcggc cgcgggatcc ttactagtga
      481 aacacctaga tggagagaga cgaagtccag cgacatactg gtctctgact gagtgggtat
      541 cgtgtagatg gagtgatgac tcagccatgg ctctcagaac agcatgcctc gttccgattg
      601 acgagtcctt gaatgtacga tgcggcagga actcagtagg gcgtcgtgtc gcaaagtaca
      661 ccagcgacgg gcagccactg tgccattttg aatgtgaagc gggacatctg ttccattctg
      721 atgaaacgtg ccgatattat tttgcctgcg attgtctgcc gcgctatgcg cggcagggtc
      781 gctcacctgc gatgccccag cagtaaagac gctcggtttc cgcgaggcct taaacgtacc
      841 tgcctgacag cgaacaagac ccttccacca cgctgataga cgcgattttc ttcacaaact
      901 tcagggtgtc gtgtaccgta caagcatcga ctccaaccct ggtgttcgga gtgtggatgc
      961 gcgagtcatt gctggggcat ccaacccttg gtctagcgtc tacatgctca atccctatct
     1021 attgttttgg agtatcggcc tgctgttttt tgtcgccgaa tggctcaggc cggcgagaaa
     1081 gattacctat cgtgccgtct tctggcgcga cctcctcgcg ctgggcgcgt ataacctatc
     1141 gttcctgctg gtcgtgcagg tgaccgatcg gattccggtt ccgcagtatc tccccgttgc
     1201 cctgtataat ctgcccacag ccggaaaact cttgttgttt tacatcgtcg aagacttcgg
     1261 cctctattgg gtgcaccgtc tcatgcacac gagggtggtt tggcccattc accggtggca
     1321 tcattcaccg acgtctctgt actggttggc aggcatacgc accacgatcc cgcatatcgc
     1381 cctcttcaat gtgacctatg tcgccgcgct tccgctcctg cacgatgcct caggctgggc
     1441 gttccaggtc atcatggtgg aacatattgt gcgtaacaat tggatgcacg tgaatgtcac
     1501 ctggaagtcg tcctggttgg aatgggtgtt cgtcaccccc cgctatcatc acattcacca
     1561 cagcagcgat ccggcgcatc aagtcaaaaa tctcggcgcg ttgctcacga tctgggaccg
     1621 gttattcggg acctactaca atcctgacga tgtgactggt gaattgaagt ttggtttgag
     1681 cgaacgtgtg aacccgatac ggcttgtcat cggcgtctag cacgcctatt cagcgctaga
     1741 gagacctgct cagacgacgg cacttctcca ttacctgacc atccagccac gcccgtggtc
     1801 gagtctcacg aatctaaaca acgtttgctg taggaagctc gtggagcggg tgcgaggtta
     1861 ttgggtgccg ttctcgccga tcaccacgaa ctgcttgcgg aacccgacga ctttggcgag
     1921 atagtcccgg gtctcttggt aaggaagccc gctgcggagc cggtcataga gggtggatgg
     1981 ctgaaggccg ttgatctgtt gcagggcggt acgctgatct ttggcaaacg ttttgaacac
     2041 gttgccggcg ccggtattgt aggccgagat gacgcagtat tcacgtgaca ccatgtccgt
     2101 gacgtcgtcc aattgagaat aggtcaacac gttgagatac gcagtgccca gttcgatgtt
     2161 attgtccgga tcaaaaagat aatcccgcga cggggctttg tcttcccctt tcgctttccg
     2221 atacgcatcc cgccctccgc tggtgggcac cagttgcatc aacccgtagg cgggggcgga
     2281 gctgacggca aatggattga aattactctc tgtgcggata atcgcaaaca cgagactcgg
     2341 actgatctgg taccgctctg cgaactgcgc gaccacggtg cgatatttct cggcctgttt
     2401 gtgcgagaaa ttggtcacca tggggatgtt gacgagatgt gcggttttgg caccgttctc
     2461 ctgctcgact ggccgggtcg tgcttttgga caggagcgat tccgcgaatt gttccgcttc
     2521 cgtcggcgta cgcaccggct tgccggcctg attcacgacc agaccaagaa gatacggttc
     2581 cttatcgccg gtcagggtga tttctttgtc cgaaaacaaa tcgacgctac gcggatcgtt
     2641 gggagttagg agcgtcgtga cgacggcatt cttcaggctt gcacgcggat ccttctcgtc
     2701 gagcgtctcg atcagaatgt tcccggcatc gaagtccacg attgcccgac tgcgatagtt
     2761 ctgggtgtat ttcacatact tcttctgctc gggtaatttg acttccttgg tgccccattt
     2821 cttgccgacc tctcccgtca acgccgccat caatgcttgg aagtcgcgtt gaatggctcg
     2881 cagatctcga agcaatgcct gggggtctcg gccgtactgc tcgacccgtt gccgagcgat
     2941 atcggcaggg tccttcccgt ggacgatatc cagaacggtt ctgccggtcg tgctgccgac
     3001 agcccgctcg gcagcgccga gcatcttatc ggtggtttca cagccggaaa ataacgtggg
     3061 gacagcgaga cttagcagca acagggacat tcggcgagtg cgcatggcgg gagtatacag
     3121 aaggtgtggc cgggaggaaa agagaatttc cgaaaccgtc attattcgca tggacagggc
     3181 ccgtttcctc cggtcggcca tcgggcatag gccagtaaat ccacgtgctt gacgcctggt
     3241 tttgggggcc agtaccatac cccatcgccg ggattccggg atgaaaggcg gaggtatggg
     3301 gaacggactg gtgggtatga acacacgtgc ttcagctgcg gtatggtgct gggtaattgt
     3361 gctgttgatg acgttggccc caagccccgg ccgcgcggcc cagacgccga ccgaggccgt
     3421 gcaggtcacg atgaaggatc tgctctacct gctgaccgaa ctcaaggaaa ccagccgatc
     3481 cgctcagcga cagtgggaaa ttgaacaggt ggtgcgacgc gcctttaatt atgaagaaat
     3541 ggctgaacgg gcgttagggg aggggagcga aaagaccacg attgccgatc gccggcaatt
     3601 tacccgactc tttgtgcagg tcctccgtga cgacttagcc gataagctcc gcgattattc
     3661 tcttcctcag gtggcctatc tctccgaaca aggcgatggc acgggaactc aggtgctcgt
     3721 ggcgcctgcg gggcgagagg tcgatacgaa gatcgaattt tctgtcgtgg agcggtccgg
     3781 gcgttggctg atcaatgacg tcagcatcga cggtgccagt attgtggcga cgtatcagac
     3841 gcagttctcc cgcatcctcc gcgaaggctc tttctccgac cttatggaat tcctcaagca
     3901 gaaagccgtg gttgcttaga tcagatcttc cggacggctg ccctcatata ttcccacgat
     3961 tgtcctcatg cctcatcggc cgttgtcatc aacggcgtga ctcctgctca tgccgaacgg
     4021 atccctcggc ctccggccga attgccgcca gacattgttc ccggaatacc gtgtcgctgc
     4081 gcgagttagt ggtgcgcgtg gaccttcagc aagccgtact cataggcccg caacagcagt
     4141 ttctggcggc tgttcacatc gaacttcccg aaaatactgg tgaggtgatg gcggacggtg
     4201 acgctggaaa tacagagccg ttcggcaatg tccttattcg agagggcctg gccaatcaga
     4261 tagacgatct cccgttcccg tccggtcaat gcagtgtcgg gagatgtcga cgagaggctt
     4321 ggcggagaat catcgtcgat ggatgattcc gtgcagtgca atctgcggcg cgttcttcgt
     4381 tcatacaggg ttgtggcgtc ggggaggccg cagacgtggc tgatggtagc gagcaatacc
     4441 accgccggtt gaatggtcag gacaatgccg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnttccgtcc gaggagacct tagagcgatg acacggcggg gcttttcgtc
     4621 gcttcgcgcc ggcaatactc ataacagctg gaaagaatga ggtcgcccga gaaacgcacg
     4681 gtgccacaga cctgtcccat aagaagggcc cagtcggcca catgggcggc caactgggtc
     4741 ggaggcattc cctgggtacg aaacagatta atggccaccg tcgagaggac tattttgaga
     4801 tgtgacaggg aaggaccgta actgaacaca tattcgtgtt gtcctgtaaa gaggagttgc
     4861 acccccgttc tagccttcat gccacgatga ccgcagcggg gacaaaagag gcattcaagt
     4921 ccgctgtcgg ggtcataccc ggcccgaaca tccgtttcca tcatttggcc acaatgggtc
     4981 gcagggcaca gcatcatgat aatctccctg aagaataatc ggtgcattgc gcggttgtcg
     5041 tctcaggcgc tatggagcgc gtgagttccc gagcggccgg aaccttctcc gctttgcggg
     5101 gccgattctc cctggacgat ccgttccgcc tcgagccaat cttcatagtc gtatccgtcc
     5161 tgctgtcctc gttgctgata tctggcatac gccagctttt cgatcctggt tctgatgtcc
     5221 tcggctgtat ccgtggtctc ggcttgcggc gacggctgga gtcgatcctt tcgcttggga
     5281 atcgacctgc tggtggcccc tcgggagatg tttcgtatgg gttcgtcgcc tgcaggtgcc
     5341 tgtgcgttac gttctggcat gatgaccact ccttgccgat caggccggcc gctgagatgt
     5401 tatcgatgaa tcttgcttta ctgaagtcgt acgcagggta tgccgagcgt cgtcaggagc
     5461 accgcttcga cggggtgttc cagaggggga ctggcggatt tcgctgagag cgtatcgccg
     5521 gatcgggact ctccgctcaa gacgggatgg accggctccg agagggcaag aacgggtgcc
     5581 tggggacaga gggcgttaag ggcgcggagt gcgtccatcc ccggagtgtc gcagtgggaa
     5641 ccgcctgaat cgtgactgaa aagaatggct gccggctgaa tttcgccgag ttgcgggagt
     5701 gtggactcga ggtctcgcac gagcaccgca tgataccctc tcgttgccag gcaatcggcc
     5761 aatttgagcc cgaactgcat cgcagaatcg acgatcatga cgcgtgatct tcccgcttgc
     5821 ttgacagggt gcatcgtggg actcatcgtt ggccctcctt gttcatgacc tggtcaactg
     5881 cagctactcc caaagacgtt ttcccggccg tgacggggat gagatggcgc aaggtgctcg
     5941 atcccgatcc ggcgagggac accgtgtact gaaattcagg caggggacgc agaacgagcg
     6001 tgcctcggcg ggacaggatg tccacggcca tgaatacctg gttccaggta caggtctgga
     6061 gttgctgcgc caaggcttcc agcgtgcagg ctccgcgccg tgtgagttct cgggtaatca
     6121 atccctctat ctcagccagc gtagtcatct gatcgttcct tttcggtact ctcctgatgt
     6181 ccgggcaggg tccaattgta cggccttcgc tgctccggcc ttcgggggac gcagcagact
     6241 caatgtgacg cgacagtgct aggtgtacct cttgagaggc aggcacgcca tcgccctttg
     6301 cgcaaaatgc gcgtcgccgt tctcccagtt ctggctgtcg ccgtttatgc tacgaattgg
     6361 agaggagagg caggaacaac gtctgaacgg agtcaggaca cgagacggtt gaccagacga
     6421 ttttcgaggg cgtaatgggt caattcggta ttgttcttga actgcatttt ttcgaggatt
     6481 cgcgcacgat aggtattgat ggtgctgatg cccacgcaca ggtcatccgc gatctccttg
     6541 aggcttttcc cgaccgcaat taaacacatg acttggtatt cgcggtcgga gagcaactcg
     6601 tgctgcggtc ttcccgcctc ctcattgagg ttggcgacga gtagttccgc catggaggcg
     6661 ctcacaaatt tcccgcctcc caggatcttc ttgagcgcct ggaccaactc gttcggggca
     6721 ctgtccttgg tgaggtaccc cgaggctccc gctttgagca ttcgccgggc caactgatct
     6781 tcggggtagg cgctaagcac caggaccgga agtttcgggc agacctgctt gaggtctttg
     6841 agcgcatcga ggccgctctt tcccggcatg ccgacgtcga ggaccacgat gtcccaggtg
     6901 aatttccgga tccggtcgat catctcctct gcattccgag cttcaccgat gacggcgccc
     6961 tggaattgct cgttgaggat ttgtttgacg ccttgtcgaa cgaccgcgtg atcatccacg
     7021 accagaattt ttgtcacgat aggctccatc tgttgcgtct ggtccaagaa tggaccggtc
     7081 atgatgactc aaatctgttc ccggcttact cctgagccgg ctggtcaagc gggatcctga
     7141 ctcgcacgat tgttcccgat ccggcattgc ctgcgaacgt gatgctgccg ccgagcagta
     7201 aggcgcgttc gcgcatgccg accaatccca gggactgggg atccgccaac tcggtttcgg
     7261 tgatgccgcg cccgttgtct tgcacttcaa gcaccagccc gccgccctgt tcctggagcg
     7321 tgatcttgac gacggaggcc ttcgcatggc gcaccacatt ggtcaggatt tcctgaaaaa
     7381 tccgaaacat cgcggtcgat gcggtctgcg ataggcacgc ggaccgcaga tagatatcga
     7441 gtcggcattc tatgccggtg cgtgactgaa attcgtgcgt ctgccattca atggccggaa
     7501 tcaggccgag ttgatcgagg acgatggggc gcaggtccgt ggcaattcgt cgcacggact
     7561 ggatcgtggc gtcgacgaga ccggaaatgg attccaggcg actgtgcaca gtggggggca
     7621 cactggggag ctgatcctgc aacaaggaga gttcaagttt gacgccggtc aacgcctgcc
     7681 cgagttcatc atggatctcg cgcgcgatga gaatccgttc ttcttcccgc accgattcca
     7741 ggcgggcggt cagctttctc agctgtgcat gcgaggcggt cagttgctgc tccgcccgtt
     7801 tgcggtcggt aatgtccaga aagacgacca cggcgcctgt gaccttcccc tgttcaatca
     7861 cggggaagga ggagtattcc acggggaatg acgttctgtc cttgcgccag tacacttcat
     7921 cgtcgacctt gcggcctctc ccatccacaa gcgtttcgtg gatgtggcac cgtgtccgat
     7981 gatagatgcc gccgtccggc gaggagtgat ggatccgttc atgcatgtct tggcacagca
     8041 gttcatcggg cgaataccct aacatacgcg aggcggcgga attgatgaac gtgcagcggc
     8101 cctgtcgatc gaggccatag atgccttccc cggtggattc cagcaggaga aggcgatctt
     8161 tggcgagttg ctgtcggatt tcttccgccc gctgtttatc ggtgatgtcg gtggctattc
     8221 cgcagagcgc gtaacagcgg ccctctgcgt tgcgcaaagg aaacttgacc acaatgctgg
     8281 tgtgtgggcc gtcgtcatgc aacgctgatt cttcgaattg catcggggcg tcggcttgaa
     8341 gcaccttccg gtcgttctcg cgaaagaccg ccgcttgagc cggcgcaaaa atttcctcat
     8401 cggttttccc gacgagatcc ttgttcgcca ggtgggacac cgtttcgaat tgccggttgg
     8461 cttggaggta gcgtccttct aaatctttga gaaagatcag ggcgggactg ttgtccataa
     8521 tggcccgaaa gcggttttcg ctgtccctga gcgcttcatc ggtgcgcctg gcttcggtga
     8581 tgtcgcgaaa gaccaccacg ccgcctttga tcaggcccat ctcatcccgc aggggcctgg
     8641 cggacacatc cagccagagg tcgttaccct gactccggcg tttgagaaac agggtcgagc
     8701 cggtcacgga ttccccccgg atggcccgtg cgagcggaat atcctccgcg gggtaagggg
     8761 tcacgcggtc aggacgaaag atggtgaagt cctcggacca atggccgact gttcgtttgg
     8821 ccgggccact tcccaagatc tgttctgcgg cgggattcca gacccgaaag gtgccggttt
     8881 catcggccat caccacgccg tcagccatgc tattgagtac cgattggagg aagcgtgtct
     8941 gctcgcgcag ttcttcctcc agccgcttgc ggcccacggc atatctcagc gcgcgtgcga
     9001 gaagaggggc cgaggtctgt cctttgatca gatagtcttg cgcgccggct tgaatgaggt
     9061 ggaggcctaa ctcttcatcc tccaagccgg tcatcagcac gatcggaatc ccatgtgcgg
     9121 cggcatgcat tctgatcagg gtttcaatgc cctggctgtc gggcaaagac agatcgagga
     9181 gaatgacatc gatgagggac tcgctcagac atcggattcc cgcatcgaga cggccgacgt
     9241 gcgtaagggt gaactgtcca ttgccggttt gtgcaaagag ctcgcgcacc aggcgaatgt
     9301 cgcccggatt gtcttcgatc agcaggacat ggataggccg gttggtcata ccgctcccgg
     9361 ttgatgtgag accggcagca ccacgatgcc cagccagaaa tcttcgatcg atcgcacgac
     9421 ccgcacgaac tggtccaggt cgaccggctt cgtgatgtag cagttcgcat gaagattata
     9481 actcttgagg acatcctgtt cgtcttgaga ggtagtgagc acgaccactg gaatgcgctt
     9541 caggctgtca tcggccttga tatcggcgag cacctcgcgg ccgtccttcc tcggcaggtt
     9601 taaatcgagc acgatgaggt gagggcgggg ggcggcggtg taggaaccct gccgccgcag
     9661 aaaggccaag gcttccatcc cgtccttgac gaccgtcaga tgattgatca ccttggcctc
     9721 tttcaaggcc tcgatggtca aacgaacatc tccgggattg tcttctacca gaagaatttc
     9781 aatcggcttt accagttctg gcacgaccat cgtgatggct cctttggatt gtggacgtga
     9841 atcatgcaac gcctgttgtc gcgccttctg agcgagtgac ggagggttgc tgcggagggt
     9901 cgggaatgct gaaccagaag gtcgcccctt tccccgtttc agactccacc cacatgcgac
     9961 ccccatgacg ttcgacaatc tttttgcaga tggccagacc gatgccggtt ccggggtatt
    10021 ccgccctggt atggagtcgc tggaaaatga caaaaatccg gccggcaaat tgcggatcta
    10081 tgccgattcc ttcgtcgcgt accgaaaaga gccactcatc ttctctccgc tgggcggata
    10141 tgtggatgcg cggcggctgg tccccgtgga acttaatggc gttgctcaga agattctgaa
    10201 acagctgcgc caactgtttg gcatcgcctc tgaccgtcgg cagccggtcg tgggtaatga
    10261 ccgcctggct ttctttgatg gcgctcagga gattgtccat cgcataggag agcacggctc
    10321 ccattggtgt aggctcaaac ggctggctgc ctgtgctgac ccgagaatag gcgagcaggt
    10381 cctgaatcag cttctgcata cgggtggcgc catcgacggc ataggcgatg aattcatccg
    10441 cgtcggcgtc caactggcct cgataccgct tcgccaggag ttgggtataa ctgctcacca
    10501 ttcgcagcgg ctcctgcaga tcgtgtgacg ccacataggc aaattgttgg aggtcggcgt
    10561 tggagcgtcc cagttctgcc acagattttt caaggtcctg tcgcgttttc aggagttcgg
    10621 atcgctcgcg tcccaatttc cacgaaacca ggatgccgaa tcccgtcagg ggtaggatga
    10681 cgagcaatcc gatgccgccg accgtccaca gtagccgatc gacaggcgca tagacctggt
    10741 catgcgtctg acggaacagc acaatccaat caaatcctgg aaaatcccga tagcctctgg
    10801 tcgaggcata cccggtgacg acggagtgcc cctggcgatg atggacttct tcgacaaatc
    10861 ctgattgatt ccggtcggag gctgcccggg atcgagaggg aatgtctaag atcgctgatc
    10921 ccttggggct ctccgctgag gtctgttttt cgctgatgat ggccccgtcc cgagtgagca
    10981 gcaaccaatc ataggggtct ttgccgtacc gtagtctgcc ttcctgttcg aaaatcgagc
    11041 gaagattgtc gatggggacg cggctggcca ccactccacg aaattcacct cgcggaccgt
    11101 agatcggtgc catgaatccc actgctttct gctgtggggg catggcagaa gggaccaccg
    11161 gttctaggta aatggtgcct gaccgccgaa cggcatcaaa ggctgcacgg ttgagagggg
    11221 gggggcctga cgcaggacgc atgtcggtgg ccgcgatgac cgttccgtcg gcatcggtga
    11281 ttcccagcca gctgtagtac cagtagagct ggtggtaatg ctgcagccgg gcggtcctct
    11341 cttcggatga accgtcccgg atcacgccat cgttggcaaa caactggata tcgccatacc
    11401 gctcgaacaa cacgcgatcg actgtctccg ccacggtcgc agcattcatg gcgagttcgt
    11461 ctcctcgttc tgcgaccagc atggcacgca ggaaggagag ggcgtataac ccgagggcgg
    11521 tcgcgatgaa aaagcacaga aagaggagga tcggcaggat gcgttccgtc caccggctgc
    11581 tactatcgaa tgctgtggtc tgcacggttg ttgtcgggag cgcaatcatg tcaggtgatt
    11641 agagggtaaa gccttaatag tttaacctgt tcggataggc agttgcacga ggaagccggt
    11701 gagcatccat tcatggctgc gttcgattga ctccgcgccg gcgtccgggt acgttcagac
    11761 cactctaggc gcgcaaagga caggatgttg agcatttcga cacatgtctc gattccggat
    11821 gaggagatcg agttgacggc agtgcgggcg caaggcgccg gcgggcaaca cgtcaataaa
    11881 gtctcgtccg ccattcacct ccggtttgac gtcagggcgt cctccctgcc cccgttttac
    11941 aaagaacgtg tgttggagct gcgggaccat cgtgtgtcgc gtgacggggt catcatcatc
    12001 aaagcacagg actcgcggag ccaggagcgg aaccgtgcat tggcgttgga acggctccgg
    12061 gagttgatcc aacaggcggc gattccgcgg ataccgcgtc gacctaccaa accgacgaaa
    12121 ggctctaaga aaagacggct ggaggataaa tcgaaacgcg gccagctcaa aagtttgcga
    12181 agccggcctc atgagcagtg aggcgcagga agaaaaaagg ggcgcttcgg gtgaagcgcc
    12241 ccttccagga cgagacgaac ctcgtcacca attagaagcg gttgcgtccg ccgcggtcac
    12301 cgccgccgaa ccgtccaccc ccgccgccac cggagcgagg ctcttgcggc ttcgcttcat
    12361 tgacggtgag ttgccggcca tccatgtcgg agccgttcaa cgccgtgatg gctgccttgg
    12421 cttcttccgc cgtggacatt tccacgaagc cgaagccgcg tgattgtccc gtgaacttgt
    12481 ccgtgatcac gcgcgccgat tcaacggtgc catgagcgga gaacaagttg ctcagttgcg
    12541 attcagtggc agcatacggc agtccgccga catacaattt gttacccata ggggtctcct
    12601 tttgaaatgt gacattgtcg gcgacacgag aacaggcata tgaggggaat gagcgggccg
    12661 aaggcgcggt ggtaggggcg acttgggtct gacttccgat caacttctcg gtaacaaaca
    12721 atatcggtga tgagccatca acacactgtg attctcatgc ggcccgtcat aagatcactc
    12781 tgcatcgtaa cagatgaagc aggggattac taccggtttc agggctgtcc aaaaaatact
    12841 ttgaggcgcg gcagagagct gcaaccgtag ccagacccgg ttctaattgc ctggggacgc
    12901 ggtacggagc gatgaggcca agcctaactt cgacagggta tagatcagcc acttcgacgg
    12961 gtcaaaattg taccacagcg gtccattgcg atagtcgctc gggtaggtgt ggtggtaatt
    13021 gtggtacccc tccccgaagg tcagcaagga gacgagccat gagttgcggc tcgaatcgga
    13081 ggtgccatac ggctgctcac cccagatgtg gcagaccgag ttgatgcaga aggtggaatt
    13141 caggaccgca aaggtgcgtc cgacgccggc cagcataaat cctccgattc caccctgcca
    13201 tccgttatgg aggaagccga tgacgaaggg gagggccagg cccacgacga cgatggccgc
    13261 ataattgcgg tgttgccaca tggccaccgg atcctgcatc acgcgggagc cgaatcgttc
    13321 atcgttgcag ggcacgggat tgaggagcca gccgcaatgg ctataccaga atccccgctt
    13381 ggcattgtac ggatcagcgg gatcgtcaca atgggcatga tgacgcaaat gatcggccgt
    13441 ccatttcgcg ccggagtttt gcaaggccca cccaccggcg accagcaggc aggccttgac
    13501 ccaatcggga cattcaaagc tgcgatgcgt caacaggcga tggtatccca cggtaattcc
    13561 caggccggtg atcaggtaga ggacggcgaa catcgtccaa tccagccagg aatagccata
    13621 caggattccg tacccgggca cgcaaatcac cgagcctgcg acaatcgacc agaagagaag
    13681 gtaggtccag aacttatgat gcacgcggtg gacgatgtgt gccggaacgg gggtcgggga
    13741 aacttgttgc atgtcgctac aatagtgaag cagacggttt ttcgcaagag gcgatgcgcg
    13801 atgaccggag agtgggggcg gtgcgcagat ctgttcggtg agggcgtggc gtcaaccgtc
    13861 tgccgctcgc cgcgttgaat cctgtgggta gaggaacatt tacgttgagg ccatccacgg
    13921 cccggacaag gatggtgtca ccaacagaga aggaggacag tatgcctact gggtatccac
    13981 gaaaggcgac atcgatggtg ctctggagtt gtgtcgtgat cggattgtct ggtctgtatg
    14041 caccggtgaa cgccgcaccg gatcctgcct ccacgggtca ggaacagccg gcggcgactt
    14101 ccgaagagcc cggcagcagc gggaagcgac cggaggggca ggggcgggga ggtcgtccag
    14161 gcaggaaggc ttgtgccgat gacgtgaaga aattgtgtgc gggggtcaaa gccggagagg
    14221 ggcggatcat gcaatgcctg agagagcaca cgcaagagct ctctccggct tgcgccgaca
    14281 tgatgcaaca acgaggaaag catcggccgt aacccttgta tggcattgcc gccgggcggc
    14341 gcgatgagcc gcccggtcga ccgttctcac ggttcacgcc tctccctctc catgcctgtc
    14401 gtgtgatttc gatattttat tctcgaagtg ctgcgagtgc gatacagtac ccttgataga
    14461 tacgggaggg tacgtatgag tgatactact cgtcaggaac agttttcggt cgacttgcgt
    14521 cagcacccgc gcttgcgtgt gccggcgccg tttgcctgtt cgttatcccg attgggcctg
    14581 gccaagtggc tcggtcgcgg aggcgagggg atcggtgtcg tctttgacgt atcgatgcga
    14641 ggggccaagg tgatgagcga agcaggaatc cagcgcggcg atcgagtatc ggtcagtctg
    14701 tcacttccca atcaggtctc gccgatgacg gtcgagaaag ccacggtgcg gtgggagcgg
    14761 gagcaggtct acggcttgga atttgtcgac ctgtcgccgg tggctgaaat gcgtttgcga
    14821 aagttcatct ccctggcgac aaagccggca gcctgaggct tcggtgcggc tcgcgatttc
    14881 ttccgcctgg tagagcagcc tcgatacggt cgatccttcg aacttggtgc cacgttccag
    14941 aagacggggg tatctgctcg acccctttcc cctctccttc gacattgcgc tcctcgctcc
    15001 gtttccctac aatgactgac ccgtttggcc atgtcgaacc ctcaggcaaa ccataagcgg
    15061 cgccagccac gtagtgtcta tcatccgatg tattggccca cgtgggtcgg cattacgctc
    15121 tttcgactgc tctcctggct tccctatcat gctcagcttg cgtttgggcg tcagtgtgga
    15181 cgcctgttgc acgggctgct cacgaagcgc cgggacatcg tccgcgtgaa tctcgccctc
    15241 tgttttcccg aacagtcgcc ggaggagcgc aatagggtgg cgctgcactc gttcgaatcc
    15301 atgggaatgg gtgtattgga aatcgccatg gcctggtggg cgaaagatcc ccgggcgcat
    15361 tgcgaatgca cgatcaaggg cttggagcat atccgcgacg cgctccatcg cgggcggggc
    15421 gtgctgctct gcggggcaca tttgcatgcg gcggaactag ccggtcgatt catggcgctg
    15481 gagcagccgg tcgccatcgt gtatcggccg cagaatgatg tcgtggccga tatggtcgcg
    15541 aaccgctgcc ggagccgata ttattcagaa ctgattcaac atcgggatat gcggggcatc
    15601 cttcgcgcat tagccaggaa ccaggtggtc tggtacgcgc ccgacatcga tgccggatcg
    15661 aagcgtagtg tgtttgctcc attcttcggc gtgccggcct cttcgctgac ggcgacgtct
    15721 cgcctggcca aggtcagtgg tgccgcggtc gtcccctgct tttattatcg gcggacggat
    15781 gggtccgggt atgacatcga ggtaggggaa gctctggacc attttccgtc cggcgaaatg
    15841 atcgaggatg cgacccgtgt caaccggttg atcgaagggg ctgtccgtcg cgtgcctgaa
    15901 caatatttct ggcagcatcg ccgcttcaaa agccgtcctc ccggcgaacc gccgctgtat
    15961 cggcgatagc cgccatcccc ggtacgcggg tttttctcac tgcctcatca gtcgccgtgg
    16021 tcagcgaaat catcagaaat attcggcgtt tcgcgccccc tggggtatac tgacgttggc
    16081 tgttttcaga cgacgactgc accatccgct ccgccatgga tcaagataaa caggtctctc
    16141 ccgttcgcat tgagctccgg caacatcgtc gctgcccggt ggccgagacc tgcctgctgt
    16201 catttgcccc cttcgcacca accttgagtt tcaatggcga tgcggaaggt gaagggacgg
    16261 ttctcaatct gtcgcccggc ggttgcaaaa tcagcagcga ggccggagtg aaggtgggcg
    16321 atgccatgtc gctgattatt ctccttcctg gagagactgc tcctacggcg gtcgatctgg
    16381 cgttggtgcg atggggcaag gatttgtctt ttggtgtgga attcgtctcg atgggcacag
    16441 ctgaacagga tcgattgcgc caatttctcg cctccgccgc tgtctcggag taaggtgata
    16501 ccgacgtgga tatggtgcgc gtgacgaaga ggagtgcagg tggaagaaac aagaaccttg
    16561 cgtaagtccc ggcgcgtcgc ggcggattgt cgtctcgagt tcgtcggcga agatgagatt
    16621 gaaggagagg ccgaggtgct ggacctctcg tcgaccggat gcatggcaaa atccgaccag
    16681 gcgatgccgc cgggccttcg catgaaagtc tccttattgt tgcccgacgg acatgaatgg
    16741 ccggttcgca tagaggaagc cgtggtccgg tgggcccggg gcggagagtt cggggtggaa
    16801 ttcctggtga tgcggcctcc gcaactccag cgcatccagc agttcgtgat caagcataga
    16861 cccctgggtt aagtcgcgat cgacacctga ccctctctgt cccttgcccg gttgcccccc
    16921 agcgattcgt gcctttccgc cctgagcctg ccactcccgt ttccccactc gttgctttac
    16981 gatgactgct tccctacaat ttcgccgtcg atcgcggctt caggcgcccg acatgctcct
    17041 tacacgcgag gcgattcaga agacatgttc acgtgggtcg gacggaagac catcgcgggg
    17101 tacgcctatc tggcggcgtt ggcgacgctg tttgcgcaag ccgtttttga tctgatcatt
    17161 cccccgaagc aggggcgggg ggagaccttc cgggtgttgg tcaggcagat tcttttcacg
    17221 ggagtcgacg ccctgccggt cacgacagtc atcgccctgt tgctcggcat catcattgtg
    17281 actcaggccg gtacgcagct tcccaggctc ggggcagggg ggctggtggg cagcatcatc
    17341 gtggtcaccg tgatccgcga actcggcccc ctggtcacag catttattgt ggttgggcgt
    17401 tccgggtcgg ccattgccac tgagctcggg aacatgtcgg tgacacgcga ggtggtcgcc
    17461 ttacggctca tggggatccc catcagccgg tttgtcatca tgcctcgtat ggtcggcatg
    17521 gtgctctcga tggtgtgcct gacactgtat ttcgatgtgg tggcggtctt cgggggctac
    17581 ctcgtggctg atgcgcaatt gaccattccg ctcgatgcat tcgcggagag tgtcatcagg
    17641 gccttgtcca cgacggacgt ccttatgacg gcgatcaagg gcctgggctt tggctcggcg
    17701 gttgcggcgg tctgttgtta ccatggcctg gctgtccgat cgtcctatac ggaggtgccg
    17761 caacaaacaa cccgcgcgat gatcaactct ttcgtcctgt gtctgctgat cgatgtcgtc
    17821 gtgacggtac cgttgtatct atgagcgagg cagccattat tttcgacaaa atccgcgcgc
    17881 ccatgttggg cggagaccta tggctggaac tcgatctcaa aattatggcg ggggagtgcg
    17941 tcgtctgtgt ggggccgagt cgtgccggga agagtttgat ggtggagctt gccgccggac
    18001 tgattgctcc ggaatcgggg cgggttcttg tgttgggcca gggttggagc gaggtttcgg
    18061 acgatgctcc ggggtccgta cggctcaagg tcggagtggt gctccaacag ccggggttgt
    18121 tgagcaatat gacgctcttt aacaatgtgc tgctgccgct gcgctatcat cgaagtgcca
    18181 tgccggatcg tcaccgtgaa caagtggtga tgggagagtt ggagcgatta ggtctggcct
    18241 cgctgcgtga tcggtttcct gcggagctga atcaggggga ggcccggcgg ggagccattg
    18301 cccgagccct gatattggag ccggaggtgc tcctcctcga tgatcccgtc gccggcttag
    18361 acgctgacat ggtgttggga ctcaagagct ctatcgacga acggcgggcc cagcggcccc
    18421 tcacggtcct cgcggcgttg cgcacctgct cgccattcgt tgatgccgcc gatcgcttga
    18481 ttgttcttcg cgatggccat gtggaagcgg acggaacccg tgacacggtg cgtgacctgg
    18541 cccccgaagc gttgcggagg tatgtagaat agccgtgcga tatggtcgga tagagaggtg
    18601 agtgatgatg cactattcgc atcgtctgtc tagcgggcgg ctggcgcaga tcgtgggcac
    18661 gtttgtgctg atccccgcgg tgattcttgc cgtggctggt ttttggatgt cgaggacgga
    18721 acacctgttc gagtcaaaat atctgctcaa ggccagcttg agtaagtctt acggcctcga
    18781 gccgggggcg cccatcgtcg tatccgggat tcgcatcggg cgcgtcaagc aggtggacct
    18841 caatgatcga gggaccgtcg atctgacgct tcagctcttg gcgcggtatc aggatatggt
    18901 gaaggagaat tccgagctgc acatcaccaa gagtggtgtg gtggtgggcc agacgcaagt
    18961 cgacatcgcc atgggcacgg ccagcagccc ggtattgtcc gagggctcca tcatccgtgc
    19021 cgtcgaaccg cgggacatcg gagacttgct caatgaggtc gagccggtac tgatggccgt
    19081 gaagcaaacg ctgctgcggg tagaaaccat cacgcaggat atgcagggtg gcctgaaggc
    19141 cggtgggaaa gcgcttgaac aggttgccct ggcaacgcag gagctgccgg cagtcgtcgc
    19201 ctcggtacag cggacgattg cctccgtgga acagacggcc acggcgctgc ccgccatgac
    19261 cggatccatt aagcggacgc tgagcatggt cgatcgcgtc acggccgatg tccaacagac
    19321 gaccggacgg cttccggcca ttctcgacag cgcccaaacg acgctcacca gcgtcaagcg
    19381 actctccgat tccgtgagtg aagtgagtca ggagctgggc cccatcatcc acagcgccga
    19441 gaccacgttg gcggatatgt cgattctggt gcgtggagcc aagcagacat ttcctttcaa
    19501 tcggtttgtg cagaatgccg ggccggcgcc caaggagtca ccgccaccct cgcgggaagg
    19561 gactcaaagt ttgagaggag atcagttgcg tcgatgagag tgcccacacc agcttcagga
    19621 gccacatgct gcgcggtggc attgtgtctg ctgtcgggat gcgcgagccc cccgccgccc
    19681 ccctctcctg cgccactgcc cagaccattg gccgagcagg gtgaatcgtt gcggcgtcaa
    19741 ggtgaccgtt cgttcatgcg aggtgatctg actcgcgccg ctgaagagta tgaggcggcg
    19801 cgtcggatgg cggaaagcct ggatgatacg caggggctta tcgtcgcgat caatgatgtc
    19861 gggcgcgtgg ctctcaggcg cggggacgtt ccccgggcgg tcagttcgca tgagcgagct
    19921 gtggccttgg cgcaacaacg gggagagccg gatcttctga ttgccagtct gactgacttg
    19981 gccactgcgg aacatcaatc gggtgcactc caggaggcag aacatcgata cgaacaagcg
    20041 ttagacctgg ctcagcagcg aggcgatcgt tcggctgaag cgacaatccg taacaatctc
    20101 gggttgatac ggcagaccaa gggcgacgtg gagcaggccg cgcaactgtt ccgtcaggcc
    20161 ctggccttga atcaagcact gggaaatttg gaatccgagg ccagcaacca tgtcaacctc
    20221 ggcatgttgg ccgaagcacg gcatgagtat gacgcggccg aacgggaata tgagcgagcc
    20281 ctggagttag acaaaacggc ggagcagcgg atggagattg cggcggatct cttgcggctg
    20341 gggaagattg cggatcgacg aggatttccc gatcgaggcc agacgtattc ggagcgggct
    20401 taccaaagct acctggcaca agatgcattt gccgaggccc ggtcggcgct gagtcaggct
    20461 gtggcttgcg cgaagaaact ggagcgggcc ggagacattg cgcggttgga acgggaattg
    20521 acccgtctga ccgccttgca tcccgcacgg tagccggttc ccctggcgta ccgccgcatg
    20581 cttacttgat ggcgacggct ttgacttctt tatacgtcgt gtgtccctgc aggactttct
    20641 gaatgccgtc ctgaaccagg gtcgtcatcc cgtcttccat gccggcatgc agcatctcat
    20701 cggtcttcgc tttattctga atcaacttct tcatcttgtc ggtacccaag agtaactcat
    20761 gcaacgccac gcggcctttc aggccggtgc gattgcaggc gtcgcatcct ttgccgcgat
    20821 agagacggaa gttcgcgtcg aacggcactt tgagggcgtc ccaatagtca gggccgtacc
    20881 ccaaacgcag ctcctcatac tcttccttcg aaggctgata ggcctccttg cagtctttgc
    20941 agatgcgccg ggccaaccgt tgagccagta cgcccagcat ggcgtcggcg aaactgaatg
    21001 agtcgcagcc catgtccagc aatcgggtga tggtttccac cgcgctattg gtgtgcaacg
    21061 tgctgagcac caagtgacct gtcaatgagg cttcaatgcc ggtgtctgcc gtttctttat
    21121 cccgcatttc gccgaccata atgacgtccg gatcggcgcg aaggaaggcg cgcatagccg
    21181 ccgcgaaggt gaatccgatt ttggcatgca cctggacctg gcgcaggccg tactgcgtga
    21241 tttcgaccgg gtcttctgcg gtccagatct tgatgtccgg cgtattgatg tagccgagta
    21301 ctgagtgcaa cgtggtcgtc tttccggaac cggtgggacc cacgcagaga atgatgccgt
    21361 agggtttctc ggcgatgctt ttgatctcgc gcagattacg ttccgaaaag cccattttat
    21421 cgaccggcag cggctcgctg gcggccagga tacgcatgac gacgtcttcg ttatagccgg
    21481 cagtcgggat ggtggcgacg cgcagctcga tttccttgtt ttcgccgatt ttaaatttga
    21541 tctttccgtc ctgcggctta cgccgctccg cgatgtcgag gctggccatg atcttcaggc
    21601 gcgacacgat ggcacggcga tagctcggtg gaattttcat gtactcgaag cagtcgccgt
    21661 cgacccggaa ccggacgagg gtgtcgcgtt tttcaccgta gggttcgatg tgaatgtcgg
    21721 aggtgccttg ccggaacgca tcggcaatga tttggttcgc caggcggacg atggcattat
    21781 cgttttcgtc gagtcccgcg gcggaggctt cttcctgggc ttcgagctgg gattcgttga
    21841 ccagttcgcc gagaatgtcg gacacattct cgttcatctt gttggtgacg tcgggggttc
    21901 cggtcgtgct tgccaggaag agcgagatgt cgcggcgcag actgacggca aaccggatgt
    21961 tgagccccgg gaaggtgcgc ttgatgtctt gaacgcggtc gagatcgccg ggatcgtccg
    22021 tgagaatctc aatggcggcg cgatcccgct tgagcggcat ccagtgattc ttcttcaggt
    22081 agtcgacgtt gaggttcttg ag
//
LOCUS       DAOQEW010000046        21673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_589_length_21673_cov_6.944167, whole genome shotgun sequence.
ACCESSION   DAOQEW010000046 DAOQEW010000000
VERSION     DAOQEW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21673
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_21673_cov_6.944167"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            545..1939
                     /locus_tag="PLS35_07150"
     CDS             545..1939
                     /locus_tag="PLS35_07150"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNI18471.1"
                     /translation="MDADFTPPSESDEGTGSSDGIPAELIFPGTTTDHGSLSVTTPDH
                     LLSEHILGSIPLWEAKGRPTGAERASYQKQIYRDVWRNSRLCKVSLVVYGSGDLGLPN
                     SIDLEFFRGFERWTKAALSRGEPFNQLVKISGRELLEASGKGLGGASYQEMDRFFLRM
                     AGTMIGAGRDWRQEGESGGSEKRPRSNKGIVFHIFQTVVLPGQVNAEGYVADKYEVEL
                     ATWYWMSLRSGNCIVIDHELFRDLHGSITKLLHQLLHNLFYLGRGTASQKYSELVRNW
                     QIKRHSALSLVKQQLDDAHRELLEKEFISRWEYVPIRTPEGKEFEIIWEAGPAWWATD
                     KKTREFREEMVGANHRELQQIGRELDPFLLIEQSDESNQEKDGQREASNARLLSVVLE
                     ISGKRKDPKVWEKWWKRAITSVPHPMIWRRIGEVKERRLSGQTINQGSYLLSLIRGDA
                     ARLGLPWATSDKVK"
     gene            complement(2242..3057)
                     /gene="traT"
                     /locus_tag="PLS35_07155"
     CDS             complement(2242..3057)
                     /gene="traT"
                     /locus_tag="PLS35_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNI18472.1"
                     /translation="MSPIIRRTLCSAGRAFVIVVVGIATGSIFGCAGHMTLSHEVNLT
                     SSNAIFLTPSEKKTIYLQTRNTSDNPNINFGELPNKIKAKGYAIVDNPDNAQFILQSQ
                     VVLCNKLKPGQTVDALIAGGFGGAIGVAAGSAAALSGASLSHIPILGAVGAVGGFAAS
                     KLSEDSIVACVVDAMVQERTKEEIQQSITTNSMPGPGVPQSQAGAFGFLNQQSVSPPQ
                     AGQVTQQIGEIRKGTRRFHQARIVASDQKMWLSVPEASKILSDKLEDSLAGLF"
     gene            4017..5777
                     /locus_tag="PLS35_07160"
     CDS             4017..5777
                     /locus_tag="PLS35_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18473.1"
                     /translation="MKQIVGLGGPLPSGESYCVFLYANVPLDAKTKSLFQMVSGNISL
                     ACSAGDDQWWSARAKRGEGTPYSKEAGFAFAQGIYRRLLELNESFVVDQEKSYFEEVQ
                     ELRQSDLKMRRIADAVPGAVYQYVIARDGCQRFSYISRGAVDMVGYPADLIVSDYNAV
                     WKLVLPEDMPGIMASIEDAIRRGLRWAHEFRLRLPDGRVKWLRGDSLPEVPTADGTVL
                     FHGLLTDVTERRLAEAELRFTQFATDHAADAILWVGPDRRIMYVNVRAAELLGYSREE
                     LLNLSVPDIAPLQSRIDILNDLTRLKEGEVVQYATTFRRKSGAVFPIEVSMRCLEHDG
                     EGYVCAIVRDVSERKRAESVIQEGAEALRRSQEALRALHSQLLTAQEDERRRVARELH
                     DDLGSRLGSAILKAEGKLVLLENNSSEAAVLKNVTDELRGISDRVRTIAHGLHPSILD
                     NLGLSVALKKMVKEYGSWSGMKVVSNISMEFTEVLDREVATCLYRIAQEALMNVKKHA
                     GAQQVMIALARQDDWVQLSVKDDGMGFSPEAESRRTKGLGIVAMEERIHMVEGELTVQ
                     SGKGRGAVVVARAPYREREA"
     gene            5774..6493
                     /locus_tag="PLS35_07165"
     CDS             5774..6493
                     /locus_tag="PLS35_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18474.1"
                     /translation="MTASVDSSMNSPVKPISVVFADDHALVADGMTTILYGVNHKQRD
                     YEFAVVGQASSGRGLLELVKRVPADLAFVDISMPDMSGLDAVPKVREIRPDIKIVVVT
                     MHREPEYMTRALSVGANGYLMKSCSAQEFCHAIDEVMRGNAYVTSLLGSWPGGTLPGS
                     DDEEDQLASLGMSQRQREVVTLLASGKTIKETAFQLGLSRKTVEHHKAMFRDKFGIDT
                     DADLTKFAIKHGLTGIDWIRK"
     gene            complement(6781..7245)
                     /locus_tag="PLS35_07170"
     CDS             complement(6781..7245)
                     /locus_tag="PLS35_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18475.1"
                     /translation="MASNPEIQELATEIDECAAGAFSGGRTNNLTDLALSIGAIAASA
                     IAAILVADGKSFPLFTAFVAALPALATALQQTVDFRGRATWYFIKSAQLQELSLNLKY
                     GGIVEIREAARLFGEIERRMEERWVDFVKPVMPKNSHHAAQPNQRTTSGDKQ"
     gene            complement(7444..9039)
                     /locus_tag="PLS35_07175"
     CDS             complement(7444..9039)
                     /locus_tag="PLS35_07175"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HNI18476.1"
                     /translation="MFSEDFYPTPGAVAAKMLQKINRNAVHFLEPSAGKGDLAKAILG
                     FGRTGSRYSSGSRHRVDVIELHPDLLKILQAHEELTVVGYDWLTYDGVSYYDAIVMNP
                     PFSEGALHLLRAWDFLHNGEIVCLLNQETIDNPHTEERRRLATIIAAHGSVEPLGPCF
                     RKAERPTDTPVVIVYLKKTTEDDRIHLWHTTDREQSINDDIGTPEAIPALRDTLGNMQ
                     HYYTHSLEEMFKAFAHIRKASLFMQALGTKLLPGSSIRDEADLKKILGMAQTNTTAAR
                     AEFARALRRSAWMHVFKQMDFQKWLDTKQTQELLQDLERDSTVPFTAKNIKGTLTNIF
                     LQRKRLFERSVWNVFVALTRHYKGNTTGDIGSGDGRSGWKTNDSYKVNCKLVFPYGCR
                     FWNERFDLWSTRDAGELYSDLDRVLAVLDGDKFEEIITVRDALECSFHNHGVGHRHCE
                     STFFRIRYFKKGTVHLRWKRDDLLASFNTTAAAGRQWIGTTCHNDQDSHASSPHSASN
                     TPHDWPSPAHNSPRLALTHSGDT"
     gene            complement(9526..10581)
                     /locus_tag="PLS35_07180"
     CDS             complement(9526..10581)
                     /locus_tag="PLS35_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18477.1"
                     /translation="MSITPDAIARARNINLIEFLISRGHTPVSRRPHHADFHAPYRED
                     RNPSFSVSQRQDGTWVWYDFGRNEHEGCKHHGDLIDLVQLLDHVTFAEALHRILHNTV
                     RSMATPSGPSLAPPTDKERIAHAKKLYYAARANMTPEREEELRSYFQTLALPYNQHLG
                     AVWMRLGDEGIPYVAFPLPTPNIHFMQGLMARALGEAPPTLMRVARGLKGPWILKRSN
                     APILITESIVDCLAGDELFGPSFTLCALNGLNTGERLQDYLQRLPSRIIYIALDNDAS
                     CAAMERPPHTSQPKKGPHVQKELVSLLTHMGYHVMEVLLHHNASVKDLHKLWLKRPQR
                     VSLLDLTKTGFHHSPVR"
     gene            complement(10639..11550)
                     /locus_tag="PLS35_07185"
     CDS             complement(10639..11550)
                     /locus_tag="PLS35_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18478.1"
                     /translation="MLNAESAFGSLWKNAFCLMLTYRKLGIQRRMDSATVLSIDTEPS
                     RVRVTKSILSCPEYMAITRLYSRVRTKLEKLTLPAGLRSGMYLIPVALAAEVDTIIAN
                     AEEELRQLVDVFLAQYDTRIAEEAARLKSAYRTSDYPDPDTVRAAFGLTYSYITFDLP
                     GTLETISATMLKREERRSMEKVNNMLEDVNTYLREAMRSLVNHLVDRLSPSDTGKSKT
                     FKSSTLTNLLEFLNTFEARNLGNDVELAQLVSSAKNVLSGVNSQLLRSDQSIAGVVQT
                     GMGTIKDQLDSLLVKRPVRLYNFDEAA"
     gene            complement(11562..11765)
                     /locus_tag="PLS35_07190"
     CDS             complement(11562..11765)
                     /locus_tag="PLS35_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18479.1"
                     /translation="MQTDSIRITIETDGTIKLVTDEISLANHATAEDVIQQILTLSGG
                     ELERTQRLTHTHHHHHTDEHLHH"
     gene            complement(11786..12103)
                     /locus_tag="PLS35_07195"
     CDS             complement(11786..12103)
                     /locus_tag="PLS35_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18480.1"
                     /translation="MPCYLEQRVSVQLNVADFPLLIAAGTSLGWEHAINGGVLVFTTQ
                     QNQRITIAKGQATLEQRHETLITELKQAYAQTIIQTAAEQFGWTLERVEGAAQELRLT
                     RFS"
     gene            complement(12139..13692)
                     /locus_tag="PLS35_07200"
     CDS             complement(12139..13692)
                     /locus_tag="PLS35_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI18481.1"
                     /translation="MSEQYPLALDFRTARNVSVPLIAVNSLDPAATMRALAWSLVNVA
                     SASQAETDNEFGLHAFPILKWDIGHGLLPLNEEGKNWHAQYVGPSKQNTTINPAEALR
                     LLEKVPERALTFILNGHRYLSNDFFIQGLWNCRDLYKANGNTIVLLGIGFKLPPELAS
                     DVVLLNEPLPTAAQLESIIKEQLSAASLPVPDPATLGKAVDATLGLGAFTAEQEVARS
                     MQASGLNIDKLWDRKRQVIDATPGLSVWRGGNSYAQIGGVATIKRFLKQVLVSKRAPR
                     CIVWLDEIEKSLSGSTGQASDTSGVSQALLGILLSYMQDHQASGLLLVGPNGTSKSAI
                     AKATGAEANIPTIALDISGLKSSLVGSSEQQMRQALNVISAISQDKACFIATSNNISQ
                     LPPELIRRFGYGTWYVDLPSQDEREAIWTIYLAKFGLATDADRPSDHNWTGAEIERCA
                     RLSWELSIPLSEAAKYIVPTAISAKESIKALETQAHQTYLSANRDGVFDQNRDTPHHT
                     RPRTITLAQ"
     gene            complement(13784..14005)
                     /locus_tag="PLS35_07205"
     CDS             complement(13784..14005)
                     /locus_tag="PLS35_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18482.1"
                     /translation="MSAPQPIPPHTTWLQRFKRLAQLTASLHPEDPRFPIVMRLLEKC
                     DGYYRTDNHASFIETGKRIRSLMDTPPIG"
     gene            complement(14049..14750)
                     /locus_tag="PLS35_07210"
     CDS             complement(14049..14750)
                     /locus_tag="PLS35_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18483.1"
                     /translation="MKIPLHHSSDTQDVADADQALASHTVAIGRGTHRSLPNRRTGYT
                     QKATIGGHKLYIRTGEYEDGTLGEIFLDMHKEGAAFRSLMNCFAIAISLGLQYGVPLE
                     EFVESFTLMRFEPNGPVSGHAQIKMSSSILDYIMRDLAINYLDRQDLAHVQVTHDDLR
                     GDSVKPYAKSTSRHTPQPLSRAETAAPPTTLGLEEARIKGYEGDPCPECHQFTLVRRG
                     TCRTCSTCGSSKGCS"
     gene            14913..16322
                     /locus_tag="PLS35_07215"
     CDS             14913..16322
                     /locus_tag="PLS35_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HNI18484.1"
                     /translation="MSEQGIEQLRARNRGGHRDPIVDGKVLVGYGACLQDPQRREGYL
                     VIRDEDRSGHVGILGTTRIGKTRLMESIVEQDIRSHKSVVAIDPKGDIDLFSKVVQVA
                     AEAGRLDEVMLVTPIFPDYSNYLDPLANYYMEDELVSHIVSGIKVKDDYYLSIASEVS
                     QVIVSGLIKLATSRGQVPTISFNDVNQRVGYTELINFRDVLKVLNDTEELCGLIDRVC
                     HGPGIAEHFAKVSSSLRTMLSALTFGNIGRIIGKARTNEFITRLEQGKRVILVCHTGS
                     LLSPRASLIFGRVFVSMIQSLVGRLFASGRQLDPPLCVHIDEGHNVLYPGIQEIFNKA
                     GGANVWLHFYEQSGAQMSAEIGADLARSISDNINSWIYFRVNHAETAQSVEDASPLVQ
                     RPRSVVSVQGNVTLHLNEERLVLRDRVLHLPKRCFYMRTHGQWYKGKTLDSSAPYVQV
                     KFPTPAVNSPKSETPPSAA"
     gene            complement(16291..17406)
                     /locus_tag="PLS35_07220"
     CDS             complement(16291..17406)
                     /locus_tag="PLS35_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNI18485.1"
                     /translation="MTFRIANFHVRLHYTWLIGFGLITWSLAANYFHDTQADLPVSYQ
                     WASGAIATLLILVSVFLHELAHAVTARRLGYAVKTIELHILGGWTIFERELSSPRVEI
                     LVAIAGPGCSFLLTLLLYAMKADPLANFLYKVNLTLGAYNLFIPCLPMDGGRILRAVL
                     WTQTHSFAVATERTAQMSKQISSGMMAIGILGIIFQYQTLWLAIIGGILRMIAETAYR
                     TVQQSEQLTRPLQEVMIPWDRVVTLVHDTPLLELQDQFLRYGYRAYPTAEHGRITGLV
                     YYKDVRTDPKWLAPNGATITPFIRALSPDVIVHPTHTLQRALDAMLLSGSDQLLVFAG
                     NTCVGMVTRSMIARVQDAIGNPDSGPTLTRQREGFPI"
     gene            complement(17409..17999)
                     /locus_tag="PLS35_07225"
     CDS             complement(17409..17999)
                     /locus_tag="PLS35_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18486.1"
                     /translation="MKPPSTFRFEFRTQCERKDRPFVARVTWDTEAKSLARGFYAIQI
                     TPVGRFGAVGECTFDAAIGDIVEVKRGETKDREYRGFYLVVPRGYLILVAIADEVDDA
                     LKVVQYVAGQASPQDLAKGHWRFESAESSWKGRPAPIDQTRMEPHPPRPVAQLAPPDK
                     RPAKPTREPAAPTSRSSTTRSVTRISRHTPPLNKDE"
     gene            complement(17999..20110)
                     /locus_tag="PLS35_07230"
     CDS             complement(17999..20110)
                     /locus_tag="PLS35_07230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI18487.1"
                     /translation="MSAYDTNTTSVFPLPLDPPLPAEEPSEPSPLPLSPRKLPDWLHA
                     HIHTPNDLTQERHSAVELNLATISPLIQNAQLNPEQLAVVTAPAEPTLVLAGAGSGKT
                     HTLIHRVAHLVTQGLPPDQILLITFTRKAAEEMIHRTQLLLQQHSLKIQGGTFHAMGL
                     IWLKHYGGALGLPPKFSIMDEDDAQGLVHLLMSKLSFSRRKGFPKKQHILALFSRAVN
                     TLRSIADTVSRDFPWLDAHTPQLVVLFRAYTETKWRQHRVDYDDLLYLVYKLLCLHEP
                     SRRRLGTHYQAVLVDEYQDTTKLQSEIVRLLGSHHHNVMVVGDDAQSIYGFRGAHTKN
                     MLEFQDAFPSVRLYKLEHNYRSTAPILTLANTVLQDAKDLYPKRLFTAKAGGQSPELI
                     ACYSEQAQSDLLTERILQCHDEGIPLHRMAVLFRSSAHSNNLELTLARHKLAFVKYGG
                     LQLTETAHFKDVLAYLRAADNPLDSNAWHRLLLLLDRVGPKLAERIIATMQEAPHPLD
                     VLDQVPGKAESTIRLLNNVIRTTQDESISLTDRLTFVIKHYEPILKRDYPEDAKVRIE
                     DLKPLCDLARQSTSLAAFLEDLTLNPSATSSSKHAHPDHHLVLSTIHSAKGLEWDVVF
                     VIWVLDGQFPPVRSWHEEQALEEERRLLYVAITRPREQLFLTYPTEHYHNASKQNLDS
                     LCRFLAPIPDDILPSYEMDQA"
     gene            complement(20378..21007)
                     /locus_tag="PLS35_07235"
     CDS             complement(20378..21007)
                     /locus_tag="PLS35_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18488.1"
                     /translation="MQCSSPESFNSVDQAHSADDHRSALRTTLSKIALYNFAAEHRLD
                     TPIELAERLILCVSLANEDRPLTRLELRQITMINPFTIDSALINLVQREEIHMLSQRT
                     SEQAVYGLTPLGHTVTVSLDSWTTSFYQYARCGSVAGVRDLVHQLQQAIRNMIATDSL
                     PIIRTCIACEYFSSAAHALDPQTAGYCRFLRTPLSIQTTKSAAPGIHTP"
     gene            complement(21126..>21673)
                     /locus_tag="PLS35_07240"
     CDS             complement(21126..>21673)
                     /locus_tag="PLS35_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18489.1"
                     /translation="VEPEAPDAPIPKQHKQLIRQMNQMTKDFERRIALQKKELEAVRE
                     HNISLSRQLREQSDEKDQLNENLASLQRQLTSENLDHKAERILRLQHKGRRQGPAIEE
                     AGPLIAGLLHTRKTVLVLQTYAQQMTTLTTPDQTTILDHINLTNQALGAIEQVLPRPT
                     DPLQSIPRPSRSVNGDRSADS"
BASE COUNT         4489 a         5198 c         6934 g         5052 t
ORIGIN      
        1 gtgggttcat gctgaccatt ctctttcatt ccaacaagcc gcatgaaatc ccaattattg
       61 aggaggcgct caaacaggcg gcagcgcgta tacaggagtt tcgtgacaag ctgacctagc
      121 gtgagatgtt caatgaaaag tttcattcaa cttacgaacg gacgggcggc gttctcatcc
      181 ccgttgggaa ggttttcttc caccttcaaa tattccagtc tttttgaaga tttactgtat
      241 taccggatcg ggggcgaata tcgatgtggt gcgcgatcct agttatccac agccctatcg
      301 ctagttgagt gaagatttcg cgttagttcg atgaaggtct cgcaagttga atgaagaaac
      361 cggctccatt ttcacgtaag ttgaatgaaa gccgacgtaa gttgaatgaa gtcacgctac
      421 ttcgatgaac gacacagttt ttataaagca ttgattgcgc aacgaaaaac aagaattaag
      481 cgactgataa actgtgtctc tacgttagaa acttacgaaa gaattaataa agaacgttac
      541 gctgatggac gctgatttca caccgccttc ggaatcggac gaggggactg gttcctctga
      601 cgggattccc gctgaactga tcttccctgg aaccaccacg gatcacggct ctctatcggt
      661 caccaccccc gaccacctcc tcagcgagca tatcttagga tcaattccac tgtgggaagc
      721 gaaaggccgc ccaacaggtg cggaacgagc ctcgtaccaa aaacagattt atcgagatgt
      781 gtggcgtaac agtcggttat gtaaagtctc cctagtggtc tacggcagcg gcgatttagg
      841 gcttccgaat tcgattgatc tggagttctt tcgagggttt gaacgatgga cgaaagccgc
      901 gctgagtagg ggagagccat ttaatcagtt agtaaagatt tcaggtcgtg aattgttgga
      961 ggcgagcggg aaaggccttg gcggtgcctc gtaccaggaa atggatcggt tctttctgcg
     1021 tatggccggc acgatgatcg gcgccgggcg agattggcga caagaaggcg aatcgggggg
     1081 aagcgagaaa cgtcctcgtt cgaacaaggg aatcgtcttt catatttttc aaacggtggt
     1141 gttaccgggg caagtcaatg cggaaggcta tgtggcggat aagtatgagg tcgagcttgc
     1201 cacgtggtac tggatgagcc tgaggtcggg caattgtatc gtcatcgatc atgaactctt
     1261 tcgggacctc catggatcga ttacaaagct gttgcatcag ttactgcaca acctgtttta
     1321 ccttgggcgt ggaacggcct cacaaaagta ttccgagctg gtgcggaatt ggcagatcaa
     1381 gcggcatagc gctctatcat tggtaaaaca gcaattagac gacgcacatc gcgagctgct
     1441 ggaaaaggag tttatttcac gctgggagta tgtgccgatt cgaacgccgg agggtaagga
     1501 gtttgagatt atttgggagg cgggcccggc ctggtgggcg acggacaaga agacgcggga
     1561 gttccgtgaa gagatggtgg gagccaatca ccgcgagttg caacagatcg gacgagagct
     1621 ggatccgttc ctcctcatcg agcaatctga tgagagcaat caggagaagg atgggcaacg
     1681 agaagcctcg aatgcgcggc tcctgagcgt cgtgctagag atctccggaa agcggaagga
     1741 ccccaaggta tgggagaaat ggtggaagcg tgcgatcacc agcgttcccc atccgatgat
     1801 ctggcgacga atcggggaag tgaaggaacg tcgattatct ggacagacga tcaaccaagg
     1861 aagttatctc ctcagtctca ttcggggtga tgctgcccgg ctggggctcc cttgggccac
     1921 atccgacaag gttaagtaac tccagtcttt cgtgccttca atcggtgccg gtgagcgagg
     1981 gactgttggc ggactaccag tgagcgatgg ggcgccatgg agatcgggat gtcctgggaa
     2041 acgggatcct tttggacagg atgacggtcc acataatggt ccaggtgagg agactcgtcc
     2101 agtaagcaag aatggggtca tccttaagga ttcaaatggt gaaaaacagc atggtctcca
     2161 gatctgagcg gatcgatcat tgagcgaggc gctgtctgcg cctcgctgag agtgagcagt
     2221 ccttgttttc aaatcggggc atcagaagag gcctgccaat gaatcctcca gtttgtcgct
     2281 gagaatcttg ctggcttccg gaacggacag ccacatcttt tgatccgacg ccacaattct
     2341 cgcttgatgg aagcggcgag tgcctttccg gatctcgccg atttgttgag tgacctgccc
     2401 tgcctgaggc ggggagacac tctgctgatt cagaaagccg aaggccccgg cttgtgactg
     2461 tggaacccct gggccgggca tgctgttcgt cgtgatggat tgttgaattt cttccttggt
     2521 gcgctcctgt accatcgcgt cgaccacgca ggcgacgata gaatcttctg agagtttgct
     2581 cgcggcaaat cctccgacgg ctcccaccgc cccgagtatc ggaatatggc tgagtgaggc
     2641 cccgctgagc gcggcggcag atccggctgc gaccccgatg gctcctccga atcccccggc
     2701 gatcaaggca tcgacggtct gccccggctt cagtttattg cagagcacca cctggctttg
     2761 aaggatgaac tgagcgttgt cggggttatc aacgattgcg taccccttgg ctttgatttt
     2821 gttggggagt tccccaaagt tgatattagg gttgtcgctg gtatttcgag tctggagata
     2881 gatagtcttc ttctccgacg gggtcagaaa gatggcgtta ctgctggtga gattgacctc
     2941 atggcttagc gtcatgtggc cagcacagcc aaagatcgat ccggtggcaa tacccacaac
     3001 aacaatgacg aaggctctgc cggctgaaca gagggtgcga cggataatcg gtgacatgct
     3061 ttcctcctgg attaggtgaa cgagtctgaa ccgcttctgc tatgtattgc cggatagcaa
     3121 cagagtgcct tttgggatat taggtgggac agcgatcaca cagaatggac cggacctcaa
     3181 agatgtttct atttggaaca actggaaatg gagcagtgtt ctgcggtaag agattcgctt
     3241 gaagtggacg ttgagtattg agtcatcgaa cgattgttgc catactgtga cggttgaatt
     3301 atgaagtctg atagcgtgga aatacgtatt gccggatgac tctctcaagt gccaccggca
     3361 ttctctgacg ggaaatcgca tcgtgcatca gccaactgcg gtgtgaggcg gtaggcggga
     3421 aggtacaggt cggcatgtca ttaggcatca aaggccaaga ctcgacgttt gatctccaag
     3481 aattctcagt cggcgatgtg tgccgtcttg ggtcgaagtt gcgacgtata gagacagcgg
     3541 gaagatcgtc agaagagatc tcgcagtcga tcgcggagtg tctggtgcgt tcgttttatt
     3601 ctcacgaact tcgtcgctcg caactgtctc ttgtggaggt gtttcaaacg cgaaatcttg
     3661 gtgagatccc gatccaccgt caagagcgat gggtacagac ggctgatggg gcgcacgctt
     3721 ctgctcaaac ccgcgttctc gcattgatgg gggtggctgg agactctgtg gaatggacag
     3781 tcggaacgcc tccgttcgga cgacattgca tccaccttca ggaggagttc gattcagaag
     3841 aagcagcgtg gtacacgcaa tcgtggaaag agttgggcct gaacaggcca gtgaactatt
     3901 tggggagcga ttgcgtcgtt acggattcac acaatcaatt gtttgaaatt agtgtgcatg
     3961 aagttcctgt ttcaccgaaa ctcggaaaga gaacagagct tctgcggacg catgaggtga
     4021 aacagattgt tggcctgggg ggaccgttgc cttctggaga aagttactgt gtcttcctct
     4081 atgccaatgt gccgctcgat gcgaaaacta agtccctgtt tcaaatggtg tcggggaata
     4141 tttctctggc gtgttcggct ggagatgacc agtggtggag tgctcgggcc aagcgtggtg
     4201 aagggacgcc atattcgaag gaggctggct tcgcgtttgc ccaagggatc tatcgccgac
     4261 ttctcgagct gaatgagtcg ttcgttgtgg atcaagagaa gagctatttt gaggaggtgc
     4321 aagagttacg tcagagcgat cttaagatgc gacgcatcgc ggatgcggtg cctggtgcgg
     4381 tgtatcaata tgtcattgcg cgagatgggt gccagcggtt tagttacatc agtcgtgggg
     4441 ccgtggacat ggtggggtat ccggcggacc tcattgtttc ggactataac gcggtctgga
     4501 aactggtcct accagaagat atgccaggga tcatggcgtc cattgaagat gcaattcggc
     4561 gtggattgcg gtgggcgcat gagtttcgat tgcgactccc cgatggtcgg gtgaaatggt
     4621 tacgcggaga ttccctgccg gaggttccta ccgccgatgg aacggtgctg ttccacggtt
     4681 tgttgaccga tgtgactgag cggcggttgg cagaagccga gctgcggttt acgcagtttg
     4741 cgacggatca tgcggcggat gccattcttt gggtggggcc tgatcgtcgc atcatgtatg
     4801 tcaatgtgcg agcagccgag ttgttgggct attcacgtga agagctcttg aatttgtcgg
     4861 tgccggatat tgcgccgttg cagagccgaa tcgacattct taacgatctg acacggctga
     4921 aggagggcga agtagttcag tatgcgacta cttttcggag aaagtctgga gcggtcttcc
     4981 ctatcgaagt gtcgatgcga tgcttggaac acgatgggga ggggtatgtg tgtgcgatcg
     5041 tgcgcgacgt gtcggagcgg aaacgtgccg aaagtgtgat tcaagaggga gcggaggctc
     5101 tacgtcgtag ccaagaagcg ttgcgtgccc tccattccca attgctgacc gcgcaagagg
     5161 acgagcgccg tagggttgcc cgggaattgc acgatgatct agggtcgcgc ctggggagtg
     5221 caattttgaa ggctgaaggg aaattggtgt tgttggagaa caacagttct gaggccgcgg
     5281 tcctcaagaa tgtcaccgat gagctgcgag gaatcagcga tcgggtgcga acgattgcgc
     5341 acggactgca tccgtcgatt ctggacaatc tggggctctc ggtggcgcta aagaagatgg
     5401 tgaaagagta tggcagttgg tcgggtatga aggttgtcag taatatctct atggagttta
     5461 ccgaggtctt ggatcgggag gtcgcgacct gtctctatcg aattgcccag gaagccttga
     5521 tgaacgtaaa gaaacacgct ggtgcacagc aggtcatgat agcgttggcc cgacaagacg
     5581 attgggtgca gttgagtgtg aaagatgatg gaatggggtt cagccccgaa gcagaatcgc
     5641 gtcgaacgaa aggactgggt attgtcgcga tggaagagcg tatccatatg gttgaagggg
     5701 aattgacggt tcagtcagga aaagggcgag gtgctgtcgt agtggctcgg gcaccttaca
     5761 gggagcgtga agcatgacgg ctagtgtgga ttcgtcgatg aactctccgg tcaaaccgat
     5821 aagcgtggtt ttcgctgacg atcatgcgtt ggtcgcagat ggaatgacca ccatcttata
     5881 tggtgtcaac cataagcagc gagactacga atttgccgtg gtgggccaag cgtcctcagg
     5941 acgtgggttg ctggagctcg tgaagcgggt tcctgcggat ttggcgtttg ttgatatctc
     6001 gatgccggat atgtcgggtc ttgatgccgt gccaaaggtc cgggagatcc ggcccgacat
     6061 taagattgtc gtcgtgacca tgcatagaga acccgaatac atgacccgag cgttatctgt
     6121 gggggcaaac gggtatctga tgaagagttg cagcgctcag gagttttgtc atgcgatcga
     6181 tgaggtaatg agaggcaacg cgtatgtgac gagcctgctt ggaagttggc caggaggcac
     6241 tcttcctggt tctgatgacg aagaggatca gctcgcctcc ctggggatgt cgcagcggca
     6301 gcgtgaagtc gtaacgttgt tggcttcagg caagacgatc aaagaaaccg cgtttcagtt
     6361 ggggctgagt cgaaagaccg ttgaacacca caaggcgatg ttcagggata aattcggcat
     6421 tgatacagac gccgacctga cgaagtttgc gatcaagcat ggtctgaccg ggatcgattg
     6481 gatccggaag taactgcgcg aaccgatggg ggcattcatt cttcaccgca gaatcggtgg
     6541 caccgcaatc aacttctttt cgctctctgc aacgtcgttc agtgccgaac acccgcagat
     6601 ctggatcagt gaatctaggt caaacccgag atcgactctg tcacatcgac ttccttgacg
     6661 tgtccttcca tcgtataggc tcaggtctcg aatgccattg attagcccgt tcccggggga
     6721 gaaagaacag cccgcgggat gcttgcatcg tcgatgcgaa gcttctccag accttgatgc
     6781 ctactgctta tctcctgaag ttgtccgttg gttgggttgg gcagcgtgat gtgagttttt
     6841 gggcatgacg ggtttgacaa agtccaccca tcgttcttcc atccttctct cgatctcgcc
     6901 aaacaacctc gcggcctctc gtatttcaac aataccgccg tacttcagat tgagagacag
     6961 ttcttgaagc tgcgccgatt taatgaaata ccacgttgct cgtccgcgga agtcgactgt
     7021 ttgctgcaac gcagtggcga gggcaggaag ggccgctacg aacgctgtga atagagggaa
     7081 acttttcccg tctgcgacaa gaatggcggc gattgcactg gccgcaatgg caccgatcga
     7141 caaagctaga tcagtgaggt tgttggtccg gccgccagaa aatgcacccg cagcacattc
     7201 atcaatttca gtcgctagtt cttggatctc cgggttcgac gccattcgaa tcctccacgc
     7261 tgagatttac ctcgcggaca agtataaagg cggcacccat tatgctcctt attcatcatg
     7321 gtttgcagtt gagcgttcac cggcggatat gtttccacga tctgagatct tacgagtggc
     7381 ttcacgttta tgcgctcttc gatctctcac gcatcgattt ccccagggac tacccgatcc
     7441 tggctaggtg tctcctgaat gtgttagggc gagacgtggg gaattatgtg caggcgatgg
     7501 ccagtcgtga ggggtattgc tggctgaatg aggagatgag gcatgggagt cctgatcatt
     7561 gtggcaggtg gtcccgatcc actgacgccc tgcggctgcg gttgtgttga agctggctaa
     7621 caggtcatcc cgtttccatc tgagatgcac tgtacctttt ttgaagtagc ggatacgaaa
     7681 gaaggtactc tcgcagtggc gatggcccac cccgtggttg tgaaatgagc attctagtgc
     7741 atcccgtacg gtgataatct cctcgaattt gtccccatca agcacagcga gcacgcgatc
     7801 caggtcggag tagagctcgc cggcgtcacg ggtggaccac aggtcgaagc gttcattcca
     7861 aaaacgacag ccgtagggaa acacaagttt gcaattgacc ttgtacgagt cgttggtttt
     7921 ccagccggac ctgccgtcac cacttccgat gtccccggtg gtattgccct tgtagtgccg
     7981 ggtgagcgca acgaacacgt tccagacaga tcgttcgaag agtcgtttgc gctgtagaaa
     8041 aatgttcgtg agggtgccct tgatgttttt tgctgtaaag gggacggtac tgtctcgctc
     8101 caggtcttgt aagagttcct gggtctgctt cgtatccagc cacttctgaa agtccatttg
     8161 tttaaacacg tgcatccagg cgcttcgtcg aagggcccgt gcgaattcgg cgcgtgcggc
     8221 ggtggtgttg gtttgtgcca tgcccaggat ctttttgaga tcggcttcgt cccgtattga
     8281 gctgccgggg agaagcttgg tcccgagcgc ttgcataaat agactggctt tgcggatatg
     8341 ggcgaaggcc ttaaacatct cctccaggga gtgtgtgtaa tagtgctgca tattaccaag
     8401 cgtgtcgcgg agcgcgggga tcgcctcagg ggtgccgatg tcgtcgttaa tagattgttc
     8461 gcggtcggtt gtatgccaaa gatggatgcg atcatcctct gtcgtcttct tgaggtagac
     8521 aatgacgact ggagtgtcgg tgggccgttc cgccttgcgg aagcagggac caaggggctc
     8581 caccgagcca tgtgcagcga taatcgtggc cagccggcgg cgttcttccg tatggggatt
     8641 atcgatagtt tcttgattca ggaggcagac aatttcgccg ttgtgaagaa agtcccaagc
     8701 acggagcaga tggagggcgc cttcgctgaa gggaggattc atcacgatgg cgtcgtagta
     8761 ggagacgccg tcgtaggtca accaatcgta gccgacgacg gtcagctcct catgggcttg
     8821 gagaatcttc aagagatcag ggtggagttc gatcacgtcg actcggtggc gtgacccgga
     8881 actgtagcgg gacccagtcc ggccgaatcc cagaatggcc ttggcgagat cgcctttgcc
     8941 ggcgcttggc tcaagaaaat gaacggcatt acggttgatt ttctggagca tcttcgccgc
     9001 aacggcgccg ggtgtagggt aaaagtcttc gctgaacata ccgctccttt tcgacagaca
     9061 caggcaagtg tgaaaggaat aggctggcgc ttttgaggaa ccctctgggc acagcactcc
     9121 aagccaggag tgctgggtga atccggcacg ggtgattact gcttcctcgc cagcgcgagg
     9181 atcttccgtc cctcttggat cgtctggaag ctgagtctgt aagcgcatac acgttcgatc
     9241 caggcgaaca tccctggtgc attgcagagg agggcggcat tggccagggc ctcatcgaca
     9301 cgatgtcggc caaggtaggc cacgccgtct tcattgagta cctggcaaat ctttccatct
     9361 cgtcggcgag gggccagaga tgtccccgca cctacgatct aagatccatg gacctggtgt
     9421 aggtttggtc attgggctcc tgtgtcctca ggagcggacg ccgtgcggtg cgtgcgacca
     9481 ggtgccgggg cgaacggctg aggccgacga aggtgaccgg ctacgctagc gtacgggtga
     9541 gtggtgaaaa cctgtttttg tcaggtcgag cagcgacacg cgttgagggc gcttcaacca
     9601 gagcttgtga agatctttca cgctggcgtt gtgatgcagg agtacttcca tgacgtgata
     9661 gcccatgtgc gtcagcagtg agaccagttc cttctggacg tgtggacctt ttttgggctg
     9721 gctggtgtgg ggtggacgtt ccatggcggc acagcttgcg tcgttatcca gcgcgatata
     9781 gatgattcgt gaagggagtc gctggagata gtcctggagg cgctcgcccg tattcagccc
     9841 attcagcgca cagagtgtaa acgaagggcc gaacagttca tcgcctgcga ggcaatccac
     9901 gatgctttcc gtgatcagaa tcggcgcgtt gctgcgtttc aggatccagg gacctttgag
     9961 accacgggcg accctcataa gtgtgggtgg ggcctcgccc aaggcccgcg ccatcagtcc
    10021 ctgcatgaaa tggatgttgg gtgtgggcaa cggaaaggcc acgtaaggaa tgccttcgtc
    10081 gcccagtcgc atccatacag cgccaaggtg ttggttgtag gggagtgcta acgtctggaa
    10141 atacgaccgg agctcctctt cacgttccgg ggtcatgttg gctcgtgcgg cataatagag
    10201 cttcttggcg tgagcaattc gttctttgtc ggtcggcggg gcgagggagg gtccgctcgg
    10261 agtcgccata ctcctgacgg tgttgtgcag gatccggtgc agggcttctg cgaacgtcac
    10321 atgatcgagg agttggacga ggtcgatgag gtctccgtgg tgcttgcacc cctcatgttc
    10381 gttgcgtccg aagtcgtacc acacccaggt gccgtcctgt cgctgcgaca cagagaacga
    10441 aggatttctg tcttcgcgat agggcgcgtg aaaatcagca tggtgcggtc gtcgcgacac
    10501 cggtgtgtgg ccgcgagaga tgagaaactc aatgaggttg atatttcggg cgcgagcaat
    10561 ggcgtctgga gtaatggaca tgagcgttat cagtaccgga aagatggtgt gtcgtggtga
    10621 tggcagaggt gcctacggtc aggcggcctc gtcgaagttg tagaggcgta ccggtcgttt
    10681 cacaagaagc gaatccagct ggtccttgat cgtacccatc ccagtctgaa cgacgccggc
    10741 aatcgattga tcactgcgca gtagttgcga gttgactccg ctcaggacgt ttttggccga
    10801 gctgacgagt tgcgcgagtt cgacgtcgtt gccaaggttg cgggcttcaa aggtgttcag
    10861 gaactccaga agattggtga gcgtgctgct cttgaaggtt ttggacttgc ccgtgtcact
    10921 cggactcaat cgatcgacca ggtggttgac gagcgatcgc atggcttctc gaaggtaggt
    10981 gttcacgtcc tcgagcatgt tgttgacctt ctccatcgac cggcgttctt ctcgtttgag
    11041 catggtagcg ctgatggtct caagtgtgcc gggaagatca aaagtaatgt aggagtaggt
    11101 caggccgaac gccgcccgta ccgtgtccgg atcagggtag tcactggtcc gataggctga
    11161 cttgaggcga gccgcttctt cggcgatccg ggtatcgtat tgtgcgagaa agacatcgac
    11221 aagttgccgg agttcctcct cggcgttggc gataatggta tcaacctcgg cggcgagcgc
    11281 cacggggatc aggtacatgc ccgagcgaag gccggccggc agagtcagtt tttcgagttt
    11341 ggtccggacg cgcgagtaca gccgcgtaat ggccatatat tccggacacg acaaaatgga
    11401 cttggtcact cggacgcggc taggttctgt atcaatggaa agcaccgtgg cactgtccat
    11461 ccggcgttga atgccgagct ttcgatacgt gagcatgaga cagaaagcgt tcttccagag
    11521 ggaaccgaag gcagattcag cattgagcat gatgacctcg tttagtggtg aagatgttcg
    11581 tcggtgtgat gatggtgatg cgtgtgtgtc aagcgctgag tgcgttctag ttccccgcct
    11641 gagagtgtga ggatctgttg gataacgtct tcggcggtgg cgtggttggc caggctgatc
    11701 tcgtcggtga ccagcttgat ggtgccgtct gtctcaatcg tgatgcgtat gctgtcggtc
    11761 tgcatgggga agtcgctcca acgggttagg agaaacgagt gagtcgcaat tcctgcgctg
    11821 cgccttcgac gcgctctaac gtccacccga actgttcagc ggccgtttga ataatggttt
    11881 gtgcataggc ttgctttagt tcggtaatca gggtttcgtg tcgttgctcc agcgtagcct
    11941 gtcctttggc aatggtgatt cgctgattct gttgagtggt aaagaccagg accccgccat
    12001 tgatggcatg ttcccatccg agggaggtgc ccgcggcgat gaggaggggg aaatcggcca
    12061 cattcaattg caccgagact cgttgctcga gatagcaggg catcgtgtgt tcctttgctt
    12121 taagttgaga cggccggctc attgcgcgag cgtaatggtg cgagggcgtg tgtgatgcgg
    12181 cgtgtcgcgg ttctggtcaa agacgccgtc gcggttggcg ctcagatagg tttgatgcgc
    12241 ctgagtttcg agggctttaa tgctctcctt ggcactgatg gcagtgggaa cgatgtactt
    12301 ggctgcctcg gacagtggaa tggagagttc ccagctgagt cgcgcgcaac gctcaatttc
    12361 cgcgcctgtc cagttgtgat cactcggtcg gtcggcgtca gtggcgagac cgaatttggc
    12421 gagatagatg gtccagatcg cctcgcgctc atcctgactt ggaagatcca cataccacgt
    12481 gccgtatccg aatcgacgga tgagttcggg cggtaactga ctgatattgt tggacgttgc
    12541 aatgaagcag gccttgtcct gagagatggc agagatgaca ttcagcgctt gccgcatctg
    12601 ttgttcactc gagccgacca gagaggactt gagaccggaa atgtctaaag cgatggtggg
    12661 aatgttcgcc tcggctcccg tggccttcgc gatggcactc ttcgaggttc cgttggggcc
    12721 gacgaggagt aatcctgatg cctggtggtc ctgcatgtag ctcagcagaa tcccgagtaa
    12781 ggcttgagag actcctgagg tgtctgaggc ctgtccggtc gatccggaga ggctcttttc
    12841 aatttcgtcg agccacacga tgcaacgggg agcccgtttg gagaccaaca cctgcttgag
    12901 aaagcgcttg atggtcgcga cccctccgat ctgggcgtag ctgtttccgc ctcgccagac
    12961 gctcagccct ggtgtcgcat cgatgacctg tcgtttgcgg tcccacagct tgtcgatgtt
    13021 cagcccgctc gcttgcatgg agcgagcaac ttcttgttcg gcggtaaagg cgccaaggcc
    13081 tagggtggca tcgacggctt tgcccagcgt ggcgggatcg gggacgggca gcgatgcggc
    13141 cgacagctgc tctttgatga tggattccaa ctgtgcggcc gtagggagcg gttcgttcag
    13201 gagcaccacg tcgctggcca attccggagg cagtttgaac ccgatgccca ggaggacgat
    13261 cgtgttgccg ttggccttat agaggtcgcg gcaattccag aggccctgga tgaagaaatc
    13321 gttggagagg tagcggtgcc cgttcagaat gaaagtgagc gcgcgttcag gaaccttttc
    13381 gaggaggcgg agcgcctctg cagggttgat ggtcgtattc tgtttcgagg gcccgacata
    13441 ttgggcatgc cagttcttgc cttcctcgtt gagagggagc aggccatggc caatgtccca
    13501 cttgaggatg ggaaaggcgt ggaggccgaa ttcgttgtcg gtctcagctt gtgaggcgct
    13561 tgcgacattg accaatgacc aggccagggc tcgcatggtg gcggctgggt cgagcgagtt
    13621 cacggcaatg agtgggacac tgacgttgcg ggcagtacga aaatcgaggg ccagcgggta
    13681 ttgttcagac atgggggatc ctttggtttc agagcgtgag agggcgtgat ccacgaaata
    13741 gtgcagagcg cacgttggtg gcgcggtcgc ttcgggagtg gcgttagccg atgggtgggg
    13801 tgtccatcag cgaacggatg cgtttacctg tttcgataaa cgaggcatgg ttgtccgtcc
    13861 ggtagtatcc gtcacacttc tcgagcagtc tcatgacaat tggaaagcga ggatcttcag
    13921 gatggagcga ggccgttaac tgggccaggc gtttgaaccg ttggagccaa gtggtgtgtg
    13981 gggggatggg ctgcggggct gacatagagg ctccatgtgt ttgagggaag agacgctcct
    14041 gggttccttc agctgcagcc cttggaactc ccgcaggtgg aacaggtacg gcacgtgcct
    14101 cgtcggacga gcgtgaattg atgacattct gggcacgggt ccccttcgta cccctttatt
    14161 ctggcttctt cgaggccgag tgtggtcggc ggggctgcag tctctgcgcg tgagaggggt
    14221 tggggcgtgt ggcggctcgt ggatttagca tagggtttga cggagtcgcc tcgaaggtcg
    14281 tcatgggtga cctggacatg agcgaggtcc tgtcggtcga ggtagttgat cgccaggtcg
    14341 cgcatgatgt agtcgaggat ggacgaggac atcttgatct gggcgtgtcc cgagacaggc
    14401 ccgttgggct cgaagcgcat taacgtgaag gactcgacga attcttcgag cgggacgccg
    14461 tattgcaacc ctaaggaaat cgcgatggca aagcagttca tgagactgcg aaatgcggcc
    14521 ccctccttgt gcatgtcgag gaagatttct cccagcgtgc cgtcctcgta ttccccggtc
    14581 ctgatataaa gcttatgtcc tccgatcgta gctttttggg tatagccggt ccggcgattg
    14641 ggcagacttc ggtgtgtgcc tctgccgatg gcgacagtgt gggacgccag cgcctggtcg
    14701 gcgtcagcga catcttgtgt gtcactggag tgatgaagag ggattttcat ggtgctcctg
    14761 ggaggtccga tccgtaaagg gtccgtatcg ttgtattact gattcgtgaa tggtcgggga
    14821 ataaagtggg tgcggctact tgaacgtgaa tatggccgat gtgctgacct ggtggagctt
    14881 ctgaccccgg taatacagca tggggaggga ccatgtcgga acaaggcatt gagcagttgc
    14941 gggcccgcaa tcgaggggga catcgagatc ccattgtgga cgggaaggtc ttggtcggct
    15001 atggcgcatg tttgcaagac ccccagcgac gagaggggta tctcgtcatc cgggatgagg
    15061 atcgttccgg gcatgtgggg attttgggga cgacgcgcat tgggaaaaca cgactgatgg
    15121 agtccattgt cgagcaggat atccggagcc acaaatcggt ggttgcgatc gatcccaaag
    15181 gggacatcga cctcttctca aaagtcgtgc aagtcgccgc agaggccggg aggctggatg
    15241 aagtcatgct ggtgacaccg attttcccag actactccaa ctacctcgat ccattggcaa
    15301 attattatat ggaagatgag ttggtcagcc atatcgtctc agggatcaag gtgaaggatg
    15361 attactatct ctctatcgcc tccgaggttt cgcaggtcat cgtctcggga ttgatcaaac
    15421 tcgcgacgag tcgggggcaa gtcccgacca tctcattcaa tgatgttaat caacgggtgg
    15481 gctacaccga attgatcaat tttcgagatg tcttaaaggt gttgaatgat acggaggagc
    15541 tgtgtggctt gatcgatcgg gtgtgccatg gtcccggcat cgcggaacat tttgccaagg
    15601 tgtcgtcgtc ccttcgcacc atgttgagcg cgctgacctt tggaaatatc ggccgcatta
    15661 tcgggaaggc gagaaccaat gaattcatca ctcgtctgga acaaggcaag cgggtgatct
    15721 tggtgtgtca taccgggtcg ttgttgagcc ctcgcgcgtc gctcattttt gggcgggtct
    15781 ttgtgtctat gattcaaagt ctcgttggcc ggctcttcgc gtccggtcgt cagttagatc
    15841 cgccgttgtg tgtgcatatc gatgaagggc ataacgtgct gtacccgggg attcaggaaa
    15901 ttttcaataa ggccggcggg gccaatgtct ggctgcactt ctacgagcaa tcgggtgcgc
    15961 aaatgtcggc cgagattggc gcggatctcg ctcggagtat cagtgacaat atcaacagct
    16021 ggatctattt cagagtcaat catgccgaga cggcacaaag cgtggaggat gcttctccgc
    16081 tggtgcaacg cccacggtcc gtcgtgtccg tacaaggcaa tgtcacgttg catctgaatg
    16141 aagaacggtt ggtgttgaga gaccgtgtct tgcatctgcc caagcggtgc ttctacatgc
    16201 ggacccatgg gcagtggtac aagggaaaga cgctggatag cagcgcgccc tacgtccaag
    16261 tgaagtttcc cactcctgcg gtcaacagtc ctaaatcgga aacccctccc tctgccgcgt
    16321 aagcgtaggg cccgagtctg gattgccgat ggcatcttgg acccgggcga tcatcgagcg
    16381 ggtcaccatc ccgacacacg tgtttcccgc aaacaccaac agctgatccg acccgctcag
    16441 gagcatcgca tcgagcgcac gttgcagggt atgagtgggg tgtacgatca catctgggga
    16501 caaggctcga atgaacgggg tgatggtcgc tccgttcggc gcgagccact tggggtctgt
    16561 gcgtacatcc ttgtaataca ccaatccggt gatccgtccg tgctcggcag tggggtaggc
    16621 acggtacccg tagcgaagaa actggtcttg cagttctagg aggggcgtat cgtggaccag
    16681 cgtcaccacc cggtcccatg gaatcatcac ctcttgcagg ggtcgggtga gctgttcgct
    16741 ctgttgcacg gtgcgatacg cggtttcggc gatcatgcgg aggatgccgc caataatcgc
    16801 aagccacagc gtttgatact ggaagatgat gccgaggatg ccgatggcca tcatgccact
    16861 gctgatttgt ttactcattt gagcggtgcg ctcagtggca accgcaaagg aatgtgtttg
    16921 ggtccataag acggcccgta agattcggcc gccgtccatc ggcaggcacg gaatgaagag
    16981 attgtaggcg ccgagcgtga ggttcacttt gtagagaaag ttcgccagcg ggtctgcttt
    17041 catcgcatag agcagcaggg taaggagaaa ggaacaacct ggacctgcga tggccacgag
    17101 aatttccacc cttgggctgg acagttcacg ctcaaaaatg gtccagcctc cgagaatgtg
    17161 caattcgatg gttttgacgg cgtagccgag tcgccgcgcg gtgaccgcat gggccaattc
    17221 gtgcaggaac accgagacga gaatgaggag ggtggcgatc gcaccggaag cccactggta
    17281 gctgacaggg aggtcagcct gcgtgtcgtg aaagtagttg gcggcgagac tccaggtgat
    17341 gagaccgaat ccgatgagcc atgtgtaatg aagccgcaca tggaaattgg cgatgcgaaa
    17401 cgtcatttct actcatcctt attcagcggg ggtgtgtgtc tggagatgcg tgtgacggag
    17461 cgcgtggtgc tgcttcgaga tgtgggggca gcgggctctc tcgtgggttt agctggtcgc
    17521 ttgtcgggcg gggccaactg cgccacaggt cgcgggggat gtggttccat tcgtgtctga
    17581 tcgatgggcg ccggtcggcc cttccacgag ctctcagccg attcgaaccg ccaatgtccc
    17641 ttcgcgaggt cctggggcga cgcctgcccg gccacatatt ggacgacttt gagggcgtca
    17701 tcgacctcat cggcaatggc gacgaggatc aaatatcctc tggggacgac gagatagaag
    17761 ccgcgatatt cccggtcttt ggtttcgccg cgtttcactt ccacgatgtc cccgattgcg
    17821 gcgtcgaagg tacattcgcc aaccgctccg aaccgaccca cgggcgtaat ttggatggca
    17881 taaaagccgc gtgccagcga tttggcctcg gtgtcccagg tgacccgagc cacaaagggt
    17941 cgatccttcc gttcgcattg ggtgcggaac tcaaagcgga atgtgctggg aggcttcatc
    18001 aggcttgatc catctcgtag ctaggaagga tgtcatctgg aatcggcgca agaaaccggc
    18061 agagcgagtc gagattctgc ttggacgcgt tgtggtagtg ctcggttggg taggtgagaa
    18121 agagctgctc tctcggccgt gtaatggcga catagaggag gcggcgttct tcttcgaggg
    18181 cctgctcttc atgccaactt ctgactggag gaaactgtcc atcgaggacc cagatgacga
    18241 agactacgtc ccattccaag cctttggcgc tgtggattgt ggagagtacg aggtggtgat
    18301 cggggtgtgc atgtttgctg ctcgaggtgg ccgaggggtt cagggtgagg tcttccaaga
    18361 acgccgccag actggtcgat tgtcgtgcga ggtcgcagag tggtttcagg tcttcgatcc
    18421 ggactttggc atcttccgga taatcgcgtt tgaggatcgg ttcgtaatgc ttgattacga
    18481 aggtgagacg atcggttaga gaaatgctct cgtcctgtgt cgtccgaatg acgttgttta
    18541 agagtctgat ggtcgattcg gctttaccag gaacttgatc gagcacgtcc agagggtgag
    18601 gagcttcctg cattgtggcg ataatccgct cggccagctt cggcccgacg cgatcgagca
    18661 gcaggaggag gcgatgccag gcattgctgt cgaggggatt gtcggccgcc cggaggtagg
    18721 caagtacgtc tttgaagtgc gccgtttcgg tgagctggag gccgccatac ttgacgaacg
    18781 cgagcttatg tcgggcgagt gtcagttcca agttgttgga gtgagcggag gagcgaaaga
    18841 ggaccgccat gcgatggagg ggaatgcctt catcatggca ttggagaatg cgctccgtga
    18901 gcaagtcgct ctgggcctgt tcggaataac aggcgatcaa ttctggagac tgtcctccgg
    18961 ccttcgcggt gaagagccgt tttggataga gatccttcgc atcctggagc actgtgttgg
    19021 ccagggtgag gatcggggcg gtgctgcgat agttgtgctc gagtttgtag agccgcacgc
    19081 tagggaaggc atcctgaaac tcgagcatat ttttcgtatg ggcgccgcga aaaccgtaga
    19141 tgctctgggc gtcgtccccg acgaccatca cgttgtggtg gtggctcccg aggagccgca
    19201 caatttcgga ttggagtttg gtcgtgtcct ggtactcgtc gaccaggacg gcctggtagt
    19261 gcgtcccgag tcggcggcgg ctgggctcat ggaggcagag taacttgtag accaggtaca
    19321 gcaggtcgtc atagtccacg cgatgttggc gccacttcgt ttcggtatag gctcgaaaga
    19381 gcacgacgag ctgaggggtg tgtgcgtcca gccacggaaa atcacgggag acggtatccg
    19441 caatcgagcg gagcgtattc acggcccggc tgaacagggc gaggatgtgt tgttttttgg
    19501 gaaacccttt ccgacgggaa aaggacagtt tgctcatcaa taagtgcacg agaccttgcg
    19561 catcgtcctc atccataatg ctgaacttgg gagggagtcc gagcgcgccg ccatagtgtt
    19621 tcagccagat caaccccatc gcgtggaagg tgccgccttg gatcttcaat gagtgctgct
    19681 ggagtagcag ttgtgttctg tggatcattt cctctgcggc cttccgagta aatgtaatga
    19741 ggaggatttg atcggggggt agaccttggg tgacgaggtg tgcaacgcga tgaataaggg
    19801 tgtgggtttt accgctgccg gcgccggcca aaaccagtgt cggttcggcc ggggcggtga
    19861 ccacagcgag ttgctcagga ttgagttgcg cattctgaat gaggggcgag atcgtggcca
    19921 ggttgagctc tacggctgag tgtcgctctt gggtaagatc gttcggggtg tggatgtgtg
    19981 catgcagcca gtccgggagt ttccgaggcg acagcgggag aggcgatggc tcagacggct
    20041 cctctgccgg tagaggagga tcgagcggga gaggaaacac ggatgttgtg ttggtgtcgt
    20101 aggcggacat gaagtaccta tgtgttactg gatctgaacg gactgaggct ttggtggaat
    20161 aaggcgacga gggtcgccgg tggcactagc ccgcattatt catgagcggt tctactgcaa
    20221 ccgctgatcc gctgctgcga cgcgccttcg gccggcgggc tcgcccctcg gaccctccac
    20281 gtactgcacg agtacgcgtc ggggcctccg gactccgcgc gccggtctcg cgacgcgcct
    20341 cagcgatttc gtgacgaccc gtcatgaata atgcgggcta gggggtgtgg atgccaggag
    20401 cggcagactt ggtcgtctgg atggaaagcg gcgtgcgcaa gaaccgacag tatccggcgg
    20461 tttgaggatc aagggcgtgg gcggcggacg agaagtattc gcaggcaatg catgtacgta
    20521 tgatcgggag tgagtcggta gcgatcatgt tccggatggc ctgctggagt tggtgaacca
    20581 ggtctcgtac cccggcgacg gagccgcagc gcgcatactg gtagaagctc gtggtccagg
    20641 agtcaagcga gactgtcacg gtgtgcccaa gcggggtaag cccatagact gcttgctcgg
    20701 acgtccgttg ggacaacatg tggatttcct cgcgctggac gaggttgatg agtgcgctat
    20761 caatcgtgaa cggattgatc atggtgattt ggcgcaattc gaggcgcgtg agcgggcgat
    20821 cctcattggc cagggacaca cacaggatca ggcgttcggc cagttcgatc ggggtatcca
    20881 atcggtgttc tgccgcaaag ttgtacaggg cgatcttgga gagggtggtg cggagtgcag
    20941 accgatggtc gtcagccgag tgggcctgat cgacggaatt gaacgattcg ggagaggaac
    21001 actgcatgtg cagattctcc gattgtcgca gctcaccagt acaagtattt gtgagtatga
    21061 acaagtgtcc aaatgcctgt tggcgaggga agggagttgg ggatctccct agggcgcacg
    21121 tatcgctagc tatcggcgga gcgatctccg ttcactgacc gcgagggtcg ggggatgctc
    21181 tggagcgggt ccgtcggcct cggcagcacc tgctcgatcg cgcccagggc ttggttggtg
    21241 aggttgatat ggtcgagaat cgtggtctgg tcaggggtgg tcagggtggt catttgctgg
    21301 gcgtaagtct ggagtactag aacggtcttg cgggtatgca agagtccggc aatcaggggg
    21361 ccggcctctt caatcgcggg gccttggcgg cgacctttgt gctgcaagcg gagaatgcgc
    21421 tcggctttat ggtcgagatt ttcgcttgtg agttgtcgct ggagcgatgc gaggttttcg
    21481 ttcaattggt ccttttcatc tgactgctcc cgcagctgac gagacaggga gatgttgtgc
    21541 tctcggacag cctccaactc cttcttctgc agagcgatcc gtcgttcaaa gtccttggtc
    21601 atctgattca tctgacggat tagctgtttg tgctgcttcg gtatgggggc gtccggagcc
    21661 tccggctcta ccg
//
LOCUS       DAOQEW010000047        21221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_613_length_21221_cov_8.451526, whole genome shotgun sequence.
ACCESSION   DAOQEW010000047 DAOQEW010000000
VERSION     DAOQEW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 21221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21221
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_613_length_21221_cov_8.451526"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLS35_07245"
     CDS             <1..359
                     /locus_tag="PLS35_07245"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNI18490.1"
                     /translation="LGPFNSGIWSGNIMSDPTLRVSSVEDLNANGFSTLTTQAHQDVI
                     GNGVWEPSGSLKGGGYTGPTWRVVVKRSLETGDANDTQFKAGASVPIAFAVWDGSNIE
                     RNGMKSLSTWFTLKLP"
     gene            633..2414
                     /locus_tag="PLS35_07250"
     CDS             633..2414
                     /locus_tag="PLS35_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI18491.1"
                     /translation="MKVSLLFPPTWHPSQPYLSLPSLTGFLTQAGVKNVSQRDLGIEL
                     LDKVLTQSFAHGLYQQLVEKQRGLERERTGETGPGSAEQLARIIESLDRFPYLFERIE
                     LAKETLRGEGFYDIEAYRNSLFLIDKWLEVLSSLYFPTRMTVVDNQFGDYSIYSSKDL
                     IKAIRDEAQNPYISLFREHVLPSILTDRPDLVGVSITATSQIIPGLTLCRLIKELAPE
                     LHVTVGGSIFTRLVDNLRRCPWLFDLVDDFVVFEGETALLELVNQMDGKRDFSKVPNL
                     IYRQNGKITVNQPFYSENINQLTAPNYDGFPLDLYLSPEPVLPVQFSRGCYYKDCAFC
                     ALTLDHQNFRQRDPGKTVDDLVWLKERYGAQSFFFTDECFALSPTKRLCQQMIDRQVN
                     VKWTCELRFEKNLSRELLTQMRDAGCLKIVFGLESFNQRVMDFMKKGIKQESVRRIVD
                     DCVDLGIAVHCYVIVGFPTEKEEEALETMNFVVGNTKLHESYGFSCQPCLFDLEKEAP
                     IMSDPGSYGIRRIMRPATEDLSLGFFYEVQEGMTPAQAEQLYQQVYEKISEVVCELPF
                     NYSMADGLLYIARAKSQALQIPQPTVS"
     gene            2495..3616
                     /locus_tag="PLS35_07255"
     CDS             2495..3616
                     /locus_tag="PLS35_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18492.1"
                     /translation="MSAIAAAVERPTGRVDDQGLLFEYTIKLVLFVGFFELVLYRLVS
                     RLGMHISKIAAQHEWIGTVFQLLTSIGFALLNVVAIFVFLALVVLLVNRARARGLTGF
                     NAVTIPSVALLLVLTVAFLIVQPAMLGSIAYNVVTIIALTALMLEYLSQQQEWSKRVM
                     GTVYYFGISGWLYYQIFSTTYGWMGVIAAPPFVYEANRIGEALMVLASILVFWAYGRG
                     VSFRTRNKQQRRRAIWFGAVSAVVFFGLLFMDYFLGLYNPVLAHSIRKAGEGISWIFQ
                     MGMGYTFYLPFAFYVAGLLCWSYTVIRLVTMGRLAGYGLALMFIAGYALLFSSLTLMV
                     ILGVMLLTLDRPKGTVTEQATVSRPPLVGTQESLVGGQI"
     gene            3649..4461
                     /locus_tag="PLS35_07260"
     CDS             3649..4461
                     /locus_tag="PLS35_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18493.1"
                     /translation="MDEHTQTGGTEQGSAAVDPGDQPLNPDEEIAEIEKLLSTEPDDF
                     QARCRLGELYFSKGRLDDALTEVKKSIEMAEGLRTEMNRSLAMYYSNLGTIYATKGMI
                     DEAEAEFKRALEVHAYDVLALFNLGRVQADKRKYMESKNYYERLVEITPDDPMAWYNL
                     AGVYVELDNPQVSDYNTMDMAIQCYLRVLELDPKHLEASFKLMEIALNHRKTDLAIKV
                     MESAVEHSPDEPLAYYNLISVYDKCKMFEQAEQARQRLKERFAKKAKEGSAS"
     gene            4486..4782
                     /gene="tatA"
                     /locus_tag="PLS35_07265"
     CDS             4486..4782
                     /gene="tatA"
                     /locus_tag="PLS35_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNI18494.1"
                     /translation="MFGSLGFTELILILFIVLIIFGAGKLPQLGEGIGKAIKGFKKSV
                     HEADAIEAEAQAQAQQQAEPVQAQAIAAQPPAPMPTPVVEQQVAAAPPAARAQA"
     gene            4828..6081
                     /locus_tag="PLS35_07270"
     CDS             4828..6081
                     /locus_tag="PLS35_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18495.1"
                     /translation="METDVQLAPGYIETFAGNGKARSTGDGKRAVKAGIPLPHHVALD
                     REEQWLYFAESGSDRVRRVNLTEGTVHNFAGIGETCYSGDEGPCGEAGLYLPLDVACD
                     SKNDLYVCDSGSNRIRKIDRETGIITTVVGTGEHGFNGDGPALEVNLTWPAAIAFDAD
                     DVMYIADTQAHRIRRFDPRTGRVETIAGSWTAEDEAREQPLVARNLVVLSGDAIGIDF
                     SDDNGWLMPVCSDGLNLSMYLDDGRPALEARLYDIVGIAVDNAGDVYVVDKGSNRVRK
                     IDRQTGLISTLAGVCRYGYDGDGKPAAKSMLHAPEAIVFDQHNHAYISDTGNHRVRKV
                     EAGTGMISTVAGNGDSGYDDKNMGGCGAARFVAKDAAGALKHGDGLLAIEAVVNSPVG
                     LTLDSQGYLYICERGENKIRRAKLW"
     gene            6169..7002
                     /locus_tag="PLS35_07275"
     CDS             6169..7002
                     /locus_tag="PLS35_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HNI18496.1"
                     /translation="MTSVHTSRTRLLSLLSVLLLLALILGGLGIPIVSSEGMIIRAQA
                     VSGDIPVAPEDPLWQKISPMTLPLSGQIITRPVWPEPTAHALTVRAVHNGTDIAFLLE
                     WQDNTKNDRLTPGTFRDGVALGLPLGDAPAFFCMGQLDHYINIWHWKADWQSDIDRRA
                     SRTTEKDKGGGSEPRRFEVIPRRASSVEDLIGGGFSTLTTKEKQGRVQGKATWKDGTW
                     RVVMRRPLSSEEQENEAKLIPGHIQAVSFAVWNGENKERNGQKAIAPWFQLALDPVTK
                     T"
     gene            7146..7565
                     /locus_tag="PLS35_07280"
     CDS             7146..7565
                     /locus_tag="PLS35_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNI18497.1"
                     /translation="MCGAALLLAATVGNPSIAAGSGSNGEMTVEEAIQLGEEFGIAVG
                     EVDEEVQKELKLQRPEGVAVFEVIGNSRADYAGIKVRSVIKEIDKKEVRNLIDFGKAV
                     KVAMKECNFTVGTYEPADPGDPVGWGVNFHFVGCKRD"
     gene            7581..8891
                     /locus_tag="PLS35_07285"
     CDS             7581..8891
                     /locus_tag="PLS35_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNI18498.1"
                     /translation="MIGTGRTIQKWAMSGLLMVFALLTVTFHERAIAQKTDGQAPADW
                     VAEIEKIFIRSEDCKQCHDRHYEEWKGMREQTPDLKSFGRVDAALLHGTSFESPVFRT
                     VLGVWMQTNPSLEERQRCLSCHAPAVTVFPQHAEKITAQVLSGKPQVEGIGCASCHLI
                     DAVETAPMPPPTYKVKPSEMLYGPYADPEENLVHPATQLPLFRGANFCTSCHFDKVKD
                     VTQKDLPGEILRGTICQDCHMEPSTGSSTSRRGAMTRSIGRHWFRGVVIPGTLLKNRN
                     LQAEWMPRIDIEATKAATGVEGTVLVKVGSLPHSFPDGDPVLKQFFLSITIKDAQGHI
                     LAEETKHFGLPYEKILRGPIPDPFIKGGNTRRVPFSQTLPAGGEEASTIEVVLNYALI
                     PEPEPELKAKYLATLPTDQERAAAKKVIEEYIQPRMLTYRTKSL"
     gene            8959..10197
                     /locus_tag="PLS35_07290"
     CDS             8959..10197
                     /locus_tag="PLS35_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNI18499.1"
                     /translation="MENRRGLLRGLIIMALLMVIGLASYGVQQGLAQDAKSQLTIEKS
                     FPSSGKCKRCHERVFEEWETSPLSKSIHTPTFRAALDAYLTSPAGKDKALCFRCHAPH
                     VREFPDHVQLFVTQAQSGEPSLDGVACAQCHLIKQVDRTKQPPEPKYDLGNKTMYGPY
                     KDFAANLAHQSMESALFHKSDLCLNCHQVVPVAADLGKSNDLLGNWDQSKAVKSGKEC
                     QTCHMPEQVGESANGEAKRKVANHTFPGRIGQLRQEAAKLDVATKVEGDKTSVTVAVQ
                     SLVPHNLPTTHPGWASVVLELTVKGKNLKTVFSDKRVYGRTYADAKGQKTVFDFEAVK
                     VLEETVLKPEETRVEAFSFPTPKDTKTFDVEAILSYAPVTGPASFLQRIEAESSKGAQ
                     DPVFQPIPIAKFSENVPVAK"
     gene            complement(10295..10549)
                     /locus_tag="PLS35_07295"
     CDS             complement(10295..10549)
                     /locus_tag="PLS35_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18500.1"
                     /translation="MGFAPEYILIDQQKFSKAKLSLDNHVYKNCEIDDCDVYYSGGQY
                     ELIDTHITNSRLILNHPAKGIYSAIQIFKMKSPGSSIISE"
     gene            10890..>12877
                     /locus_tag="PLS35_07300"
     CDS             10890..>12877
                     /locus_tag="PLS35_07300"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001951.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNI18501.1"
                     /translation="MSDSAIVTFALVAAVAGIAYGLYLAMWVFKLNAGNTKMQEISKA
                     IQEGAGAYMNRQYKTVGVVAAVLFVLLWGAGAVSDKFGMLTAVGFLIGAGASALAGYV
                     GMIIAVRANVRTAQAAHNGMNAALTVAFRGGAVTGLLLIGLGLLAITSFYTLASQLAG
                     QEKAIHALLSLGFGGSLISVFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVI
                     ADNVGDNVGDCAGMAADLFETYAVTTVAAMVLAFTMFKGVNAPILYPLALGGVTIFAT
                     IIGILFVKVSPGGEIMPALYKGLFVAGGIAAVAFFPITSQIMEGVGGVSGISYYLAAL
                     MGLAVTLALVFITDYYTSKAYAPVKDIAKASETGHATNIIAGLAVGMQSTAAPVVVIG
                     AAILASYWICGGAASGGLYGVAVAAVSMLSMAGIVVAIDAFGPITDNAGGIAEMAHLG
                     KEVRDITDPLDAVGNTTKAVTKGYAIGSAGLAAVVLFAEFAREVAKGTQASTFDLSNP
                     SVLVGLFLGGMLPFIFGALCMKAVGQAAGLVVEEVRRQFRTIKGIMEGTGKPEYGTCV
                     DIVTQAAIAKMMIPGLIPVVAPIIVGVILGPQALGGVLVGSIVTGLFVAISMTSGGGA
                     WDNAKKYIEEQGLKGTDTHKAAVTGDTVGDPYKDTAGPA"
     assembly_gap    12878..12977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13000..13197
                     /locus_tag="PLS35_07305"
     CDS             13000..13197
                     /locus_tag="PLS35_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HNI18502.1"
                     /translation="MEKQERRPESRKESHAKEEVKPNPKVVESGKKMKEDIDKLVDEI
                     DDVLEKNAEEFVKNYVQKGGE"
     gene            13400..15106
                     /gene="recJ"
                     /locus_tag="PLS35_07310"
     CDS             13400..15106
                     /gene="recJ"
                     /locus_tag="PLS35_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNI18503.1"
                     /translation="MLEKLWVFRPFDTVLQNSLVRTLSISPVTASILLARGVTTADQA
                     SRWLSSPQDGLHDPFLIPDMEAAVERLHVALAREERVCFYGDYDVDGVSATSLYLSFY
                     RGLGGNACGYIPHRVREGYGLNEGAVRKLAQEGVTLLVTSDCGTTSHHEIAVAKELGM
                     DVVVTDHHQTDATMPAALAVLNPHRRDAIYPFKGLCSAGLAYKVVTAYQRRYGSGEVD
                     PESCLDLVALATVADIVPLQDENRILVREGLIQINRGARCGIRALKQVAGITKACTSE
                     TIGFRLGPRLNAAGRLDHAMLCVQLLTTESDREAQQIAEQLEQLNRQRQQIEEEITAQ
                     AGASLQQDEPAAAIVLGSRHWHLGVVGIVAARLVDRFHRPSIVIAVDEKGLGKGSART
                     VEGFDLYQGLSACRDLLEAFGGHPSAAGLTIQESRLEEFRRRFCEVAAQWATASLVRP
                     TLHVDAEVKLTEVNFDLIQELESLHPFGAGNPEPTLAVRGLDVVEARVVGEKHLKLRV
                     RQGRSFIFDSIGFRMGSFEELGLKTGRPVDLAFSPERNHWNGYDRVQLRIKALRMSGE
                     VS"
     gene            15109..17292
                     /locus_tag="PLS35_07315"
     CDS             15109..17292
                     /locus_tag="PLS35_07315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNI18504.1"
                     /translation="MPHETVTELDQLIERVKSYNVEADLDLVRRAYDFSAKAHEGQMR
                     RSGEPYFRHPLAVAGLLTHLKTDVTAIVAGLLHDTLEDTLATPLELEQRFGKDVVHLV
                     DGVTKIGKITFRNYEEKQAENFRKMVLSMADDIRVVLIKLADRLHNMRTLEYLSEGKR
                     RQIAQETLEIYAPLANRLGIGWIKNELEDLCLKHLKPDVYEMLRVRVAKRDEDRQQYI
                     HEVIDMVKKAMAEAGLYGEVSGRPKHLYGIYQKMEKQSISFEEVYDLAALRIITDIKM
                     NCYALLGVIHSLWRPLPGRFKDYIAIPKSNMYQSLHTTVAGPKGEHVEFQIRTEEMHR
                     VSEQGIAAHWKYKEHGRIDEKDGKVFSWLRQFVEWNQDLPDNRQFMDSVKLDLFHDVV
                     YVFTPQGMVKELPKGSTPVDFAYSVHTEIGDHCVGAKVNGKIVPLKHMMESGDMVEIL
                     TAANQTPHRDWLKFVRTSRAKTKIKHWIKAEEQSRSIEIGKRLLEAEFRRHGLAPAQM
                     MRSEQLLAVAKQVGYETAEELAAAVGFGHVPTSQVMSKIAPPLPGEAQTVAPEAPVPH
                     KPVIGRTDDTGVQVKGARDLLMQLSRCCNPVPGDRILGYITRGRGLTIHTIDCPNLEA
                     LDYDKNRLVEVEWDQSTPSTHSVKVSVIAVDKTGVLAHVSTAISECQANISRAEITTR
                     EDRKAMLDFIVEVADTAHLSRVFKAIEKVEGVITVRRMRSWQERA"
     gene            17341..18858
                     /locus_tag="PLS35_07320"
     CDS             17341..18858
                     /locus_tag="PLS35_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNI18505.1"
                     /translation="MTLGTLVSSILAILACAAGVLVALTYVFTRRGKAVPVLPFYAGG
                     VGVFFVVASLIGHSGLGTSVPHFVQSCLNVAAWLGAAFFFFTILDVLIIGEWLIERGQ
                     RYIPDIVRQLLIGAEVVAAGVLILWLVMGINLVALVALPTVAAAMVGVALKDTLTRFF
                     SGIELGKIVKVGDWVTVLDREGVVTHIGMEHVTLLTRAHDVVSLPNDLVIQSGVTNFT
                     RPTTTHFCNVFVDAAYRTPPEQVCATLLETASSVSGVLPDPKPSAFVTAFNESAIQYR
                     IKFPIGDFSQRDSIESAMRTYIWHAFARTGIEIPFPQRVVHQSANGQDAAQDRYAIER
                     IMAKLTAVDFLATLDTKQIEVLAEGARGELYLPGERIVRQGDPGEVLYVIVSGTADVR
                     LEQGGLTTTVSTLEPGKFFGEMSLLTGEPRSATVVAVTQLSVIAVSKSALLQVVQEDR
                     RLIERIGEVVARRQAVTAAAKAQLSRDAAALSVVTHTRSLVERIQSFFWGTRKST"
     gene            complement(18877..19386)
                     /locus_tag="PLS35_07325"
     CDS             complement(18877..19386)
                     /locus_tag="PLS35_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNI18506.1"
                     /translation="MTQSMDHSSQTPHSTWLSRLTSCLWSLAIVASPWSGWAADLPTE
                     TLIAVKMPNGTVIQAELADTALKRAQGLMFRERLADDRGMLFIFGDAQPWTFWMKNTK
                     IPLDIIWMDAKKTIVHIERNTPICTRQDDGCPQYHSEEGALYVLELGAGRAAALQLQR
                     GVKLSFKQP"
     gene            19525..19710
                     /locus_tag="PLS35_07330"
     CDS             19525..19710
                     /locus_tag="PLS35_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18507.1"
                     /translation="MKETRRVVYMRGVFVCPKCRQSYVQEKWIEEFRIRCHKCDYRGT
                     LTQVSVEEHMDEEVIRR"
     gene            19843..20463
                     /locus_tag="PLS35_07335"
     CDS             19843..20463
                     /locus_tag="PLS35_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNI18508.1"
                     /translation="MVQMWRLLLLCATSILIGPTIGSSADSPPITQVLVAYHSLSGNT
                     ERMAAAVAEGARTVPHTEVIVRRIGSVTAEELFAADAIVLGSPVYWSNMAGEVKAFID
                     DWQFKFGVFPEFKLKNKVGAAFATGGQISSGKELTMLSILAAMLGNYMITVSGGGAFG
                     ASATTEGNSPGIDEQELGSARELGRRVADVAGAVKRGFALQERGVR"
BASE COUNT         4565 a         5437 c         6366 g         4753 t
ORIGIN      
        1 gccttgggcc attcaactcc ggtatttggt ccggtaacat catgtccgac ccgacactgc
       61 gcgtcagctc cgttgaggat ctgaatgcca acgggttcag caccttgacc acccaggccc
      121 atcaagatgt gatcggcaat ggcgtgtggg agccgtcagg ttccctcaag ggcggtggat
      181 acaccggtcc gacctggcgc gtagtcgtga agcgttcgct ggagaccggc gatgccaacg
      241 acacccagtt caaggcggga gcctctgtgc ccatcgcgtt tgcggtgtgg gacggttcca
      301 acattgaacg gaacggtatg aagtctctct ccacttggtt cacgctgaag ttgccgtgat
      361 ccaagcggtc tgaagtaaaa ttcctgcccc tgtgccgggc tggaacgtgt tgagggcttg
      421 aagcagaagg ccccgagttc ttgtcagcga tggcaaagtg actcggggcc ttcgtgcatt
      481 tctgggtctc tgtgacgcat tggcgcgaac agtggaattt ctttttcatc acagtcggag
      541 ggttgctttc ggaccaaagc ggagtgtata aaccgatacg gtacaaatct cctgaaaaca
      601 ctgattttcc tgcttaatat aaagataatt ttatgaaggt ttctctcctg tttccaccga
      661 cgtggcaccc ttcgcaacct tacctaagcc tcccttctct cactggtttc ctcactcaag
      721 cgggagtcaa aaatgtctcc cagcgtgatc tcggcatcga attgctggac aaagtcctca
      781 cccagtcctt tgcccacggc ctctatcagc agttggtcga gaagcagcga ggccttgagc
      841 gagaacgtac gggagaaact gggcccggga gtgcggagca acttgcgcgg attatcgaat
      901 cccttgatcg cttcccctac ttgtttgaac gtatcgaact tgccaaagag acattgcggg
      961 gagaaggctt ctatgacatc gaagcctatc gcaacagcct ctttctaatc gataagtggc
     1021 tcgaggtgct gtcatcgctc tatttcccca ccagaatgac cgtcgtggat aatcagtttg
     1081 gtgactactc catctattcg tcgaaagatc tgatcaaagc tatccgtgac gaagcgcaaa
     1141 atccctacat cagtctcttc cgtgagcacg tattgccctc tattttgacc gatcgtccgg
     1201 atttggttgg cgtctcgatc acggctacgt cgcaaatcat cccgggtctc acgctttgtc
     1261 gattgattaa agaacttgcg ccggaactgc acgtgaccgt cggaggcagc attttcacgc
     1321 gcctggtcga taacctgcga cgctgcccat ggctcttcga tctcgtcgac gattttgtgg
     1381 tgttcgaggg tgaaaccgcc ttgctcgaac tcgtcaatca gatggatggc aagcgtgact
     1441 ttagcaaggt gcccaatctg atttatcgtc agaatggaaa gatcaccgtc aatcagccgt
     1501 tctactccga gaatatcaac caactcacgg cgcccaacta cgacggattt ccgctcgacc
     1561 tgtatttgtc tccggagccg gtactgccag tgcaattttc gcgaggctgt tactacaagg
     1621 actgcgcgtt ttgcgcgttg acgctggatc atcaaaactt ccggcagcgc gatccgggca
     1681 agacggtgga tgatctggtc tggttgaagg aacggtatgg cgcgcaatcg tttttcttta
     1741 ccgacgaatg cttcgcactg tcgcctacga agcggctctg ccagcagatg attgatcgtc
     1801 aggtcaatgt aaagtggacc tgcgaattga ggttcgaaaa aaatctctcg cgagaattgc
     1861 tcacacagat gcgggacgcc ggctgcttga agatcgtctt tggtctggag tcgttcaacc
     1921 agcgtgtcat ggacttcatg aaaaaaggga tcaagcagga atccgttcgg cgcattgtgg
     1981 atgactgcgt cgatctgggc atcgcggtgc actgctatgt cattgtgggc ttccccacgg
     2041 agaaggagga ggaagcgctg gaaacgatga acttcgtggt aggcaatacc aagctccacg
     2101 aatcatatgg attctcttgc cagccctgcc tgtttgactt ggaaaaagaa gcgccgatca
     2161 tgagcgatcc ggggagttat ggcattcggc gaattatgcg accggccacc gaggatttga
     2221 gtctcgggtt tttctacgaa gtgcaggaag ggatgacgcc ggctcaggcg gagcagctct
     2281 atcagcaggt gtacgagaag atcagcgaag tcgtgtgcga gttgccgttt aactattcta
     2341 tggccgacgg gctgctgtat attgcccggg ctaagtcgca agcgttgcag ataccacaac
     2401 ctaccgtctc ctagattgga aagtcgggcc gcgcagagtg cggcctcatg cggtgtggtc
     2461 acgaagtgct tcacgtttgc caggggggct ggagatgtct gctatagccg ccgccgtcga
     2521 acggccaaca ggacgagttg acgatcaggg attgttgttt gaatacacca tcaagctggt
     2581 gttgttcgtc ggctttttcg aactggtgct atatcgtctg gtctcccgtc tgggcatgca
     2641 catcagcaaa attgccgctc agcatgagtg gattggaacg gtcttccagc tgctgacctc
     2701 gattgggttt gcgctgctga atgtcgtggc catttttgtg ttccttgccc tcgtggtctt
     2761 gctggtcaat cgtgcccgcg caagagggct cacgggtttt aatgccgtca ctatccccag
     2821 cgtggcgctt ctgctggtgt tgacggtggc ctttctcatc gtgcaaccgg ccatgctcgg
     2881 ctcgatcgcc tacaacgtcg tgaccatcat cgcactcacg gcgctgatgc tggagtatct
     2941 ctcgcagcag caggagtggt ccaagcgcgt catgggcacc gtctattatt tcgggatttc
     3001 cggatggctg tactatcaaa tcttctccac cacctacgga tggatgggag tgattgcggc
     3061 accgccgttt gtctatgaag ccaatcgtat cggcgaagcg ttgatggtgc tggctagtat
     3121 cctggtcttt tgggcctacg gacgcggagt atcgttccgc acgaggaaca aacagcagcg
     3181 ccgccgcgcg atctggttcg gggcagtgtc ggcagtggta tttttcggcc tgttgtttat
     3241 ggattatttc ctggggttgt acaacccggt gctggcccat tccatccgga aggccggcga
     3301 ggggatcagc tggatcttcc aaatgggcat gggctatacc ttctatctgc ccttcgcctt
     3361 ttatgtcgcc ggactgttgt gctggtcgta taccgtcatt aggctggtga cgatggggcg
     3421 cctggcaggc tacggactgg ccttaatgtt tatcgccggt tatgcgctgc tgttttccag
     3481 tttgaccttg atggtgattc ttggggtaat gttattgacg ctcgatcgcc ctaagggaac
     3541 ggtcaccgag caggcgacgg tgagtagacc gcccctggtc ggcacgcagg aatccctcgt
     3601 tggcggacag atttaatttt tgactcaccc agattaggat acgttgttat ggatgaacac
     3661 acacagaccg gcggaacaga acagggtagt gcggcggtag atcctggcga tcagccgtta
     3721 aatcccgatg aagaaattgc tgagattgaa aagctgctca gcaccgagcc cgatgatttt
     3781 caggcacgct gccgattggg tgaactgtac ttcagcaagg gccgattgga cgatgccctt
     3841 acagaagtaa agaagtccat cgagatggcc gagggcctca ggacggagat gaatcgctct
     3901 ctggcgatgt actactccaa cctcgggacc atctatgcta cgaagggcat gatcgatgag
     3961 gccgaggcgg agtttaagcg tgcgcttgag gtgcatgctt atgatgtctt ggcgctgttt
     4021 aatctcggcc gggtgcaggc ggacaagcgc aagtacatgg agtcgaagaa ctattacgaa
     4081 cgcctggtcg agatcacgcc ggacgatccc atggcctggt acaatctcgc tggcgtttac
     4141 gtcgagctcg ataatccgca agtctccgac tacaacacca tggacatggc gattcagtgt
     4201 tacctgcgtg ttcttgaact ggatccgaag cacctggaag ccagcttcaa gttgatggag
     4261 atcgcgctca atcaccgaaa gaccgatttg gcgatcaagg tgatggagag tgcggtggag
     4321 cacagcccgg acgagccgct cgcgtattac aatttgatca gcgtctacga taagtgcaaa
     4381 atgtttgaac aggccgagca ggctcgtcag cggttgaaag aacgatttgc gaagaaggcc
     4441 aaagagggat ccgcatcctg aacacgtgag caaaggagac acaccatgtt tggcagcctt
     4501 ggttttacag agttgatcct gatcctgttt atcgtgttga ttattttcgg agcagggaag
     4561 ttgccgcagc ttggtgaggg gatcggcaag gccatcaagg gctttaaaaa atccgtccat
     4621 gaagcggatg cgatcgaagc ggaagcccag gcgcaagcgc agcaacaggc cgaacccgtg
     4681 caggctcagg ccattgccgc ccaaccgccg gctccgatgc cgacgcctgt ggtggagcaa
     4741 caagtcgctg cggcccctcc tgccgcgcgc gcacaagcat aatatcctcg ataacaaggg
     4801 cccagcatag aacggacgca caacgttatg gaaacagatg ttcagttagc tcccggatac
     4861 attgaaacct ttgccgggaa tggaaaggcc aggagtacgg gagacggcaa gcgcgcggtg
     4921 aaggccggca ttccccttcc gcaccacgtc gctctggatc gggaggagca gtggctgtac
     4981 ttcgcggagt ccggatccga tcgtgtgcgt cgcgtcaact tgacggaagg tacggtccat
     5041 aacttcgccg gcatcggaga gacgtgctac agcggagacg aagggccttg tggggaagcc
     5101 ggcttgtatc tgcctctcga tgtggcctgt gattccaaga acgatctgta tgtgtgcgat
     5161 tcagggagca accggattcg caagattgat cgggagaccg gcatcatcac gacggtcgtc
     5221 gggaccggtg aacatggctt taatggagac ggaccggcgc tggaggtgaa tctcacgtgg
     5281 cctgccgcca tcgcgttcga tgcggacgat gtcatgtata tcgccgacac gcaggcacac
     5341 cggattcgtc ggttcgatcc acgaacgggc cgtgtcgaaa ctattgccgg cagctggact
     5401 gctgaggacg aagccaggga gcaacccttg gttgcgcgca atttggtcgt gttatccggc
     5461 gatgcgatcg gtatcgattt cagcgacgac aacggttggc tgatgccggt ctgttctgac
     5521 gggctcaatc tctccatgta tctggatgat ggtcggcccg ctctagaagc gcgtctctat
     5581 gatatcgtcg gaattgccgt cgacaatgcc ggggatgtgt atgtcgtaga caagggcagc
     5641 aatcgcgtgc gcaagatcga tcgccagacc ggactgattt cgacactcgc cggcgtatgc
     5701 cgctatgggt acgacggaga tggcaagccg gcagccaagt cgatgttgca cgcacccgaa
     5761 gccattgtgt tcgatcaaca caaccacgcc tatatctccg ataccggcaa ccaccgtgtt
     5821 cgcaaagtcg aggcggggac ggggatgatc agcaccgtgg ccgggaacgg cgatagcgga
     5881 tatgatgata agaatatggg tggctgcggc gctgcccgct ttgtcgcgaa agatgcggcc
     5941 ggcgcgctca aacacgggga cgggttgctc gctattgagg cggtggtgaa ttcccccgtg
     6001 ggcttgacgc tcgactccca aggatatctg tatatttgcg agcggggtga aaataagatt
     6061 cgtcgcgcca agttgtggta acgcttggag gacggagcca aggctcccgc acagggaacg
     6121 cgtgcgcgtg ttgtcacgac taaggccacg cgccgggaca tgaggcgaat gacgtcggtc
     6181 cacacatccc gcaccagatt gttgagtctc ctctccgtgt tgttgcttct cgccctcatt
     6241 ctgggtggtc tcggtatccc cattgtcagt tccgaaggca tgatcatccg tgcgcaggcc
     6301 gtctcgggag acatacccgt ggcgccggaa gatccgctct ggcagaaaat ctccccgatg
     6361 accttgccac tgagcggcca gatcattacc cgccccgtct ggccggagcc gacagcccac
     6421 gcgttgacgg tacgcgccgt gcacaatggt accgacatcg cattcttgct ggaatggcag
     6481 gacaatacga aaaatgatcg gttgacgcct ggcacatttc gagatggcgt ggctttggga
     6541 cttccgcttg gtgacgctcc ggcgtttttt tgtatgggac aattggacca ttacatcaat
     6601 atctggcact ggaaagctga ttggcagagc gacatcgatc ggcgcgcatc gagaaccacc
     6661 gaaaaagaca aggggggcgg cagtgagccc cggcggtttg aggtgattcc acgacgggcc
     6721 tcttcggtag aggatttgat cgggggcggg tttagtacgt tgaccacgaa agaaaagcag
     6781 ggtcgtgttc agggaaaagc tacctggaag gacgggacgt ggcgtgtcgt gatgcgccgt
     6841 cccctctcga gtgaagagca ggagaatgaa gcgaagctga ttcccgggca catacaagcc
     6901 gtgtccttcg ccgtgtggaa tggtgagaac aaagagcgga acgggcaaaa ggcgatcgct
     6961 ccctggtttc aacttgcatt ggatccggtc acaaagacgt aattcttcct ggtaggatga
     7021 tgtaaaaagc cgagcgcatc ggcgagagct ttgttcgcaa aggtagaggc cagcttttgg
     7081 aggacagtgt tcagattcat cgcgtgatgc acaacagagc cctttctttc ggaagggctc
     7141 tttgtgtgtg tggagcggcg ctgctccttg ccgccacggt cgggaatcct tcgattgccg
     7201 caggctcagg cagcaatggg gaaatgacag tggaggaagc cattcagctt ggagaggagt
     7261 tcggcattgc ggtgggtgag gtcgacgaag aggttcaaaa ggagctcaaa cttcaacggc
     7321 cggaaggtgt cgcggtcttc gaggtgatcg gcaattcgcg ggctgactat gcgggaatca
     7381 aggtccgctc cgttatcaaa gagattgata agaaggaagt ccgcaacctg attgatttcg
     7441 gcaaggcggt caaagtcgcc atgaaggaat gtaactttac ggtgggaacc tacgagcccg
     7501 ccgatccagg cgatccggtc ggatggggag tgaattttca tttcgtcggg tgcaagcggg
     7561 attaagagag cgagccacac gtgatcggaa cgggacgaac cattcagaaa tgggccatga
     7621 gcggactgct gatggtattt gcgctgctca cggtcacctt tcatgagcgg gctatcgcgc
     7681 aaaagaccga cggtcaagcc ccggccgatt gggtggcgga gatcgaaaag atctttatcc
     7741 gttctgaaga ttgcaaacag tgccacgatc ggcactacga agaatggaag ggcatgcggg
     7801 agcagacgcc cgacctgaaa tccttcggtc gagtggatgc ggcgttatta cacggcactt
     7861 cctttgaatc tccggtgttc cgcacggtgt tgggcgtctg gatgcagacc aatccttcgc
     7921 tggaagaacg gcagcggtgt ctatcctgcc atgcgccggc cgtcacggtc tttccccaac
     7981 atgccgaaaa aattaccgcg caggttctct caggcaagcc ccaggtggaa gggatcggtt
     8041 gtgcgtcctg tcatctgatc gatgcagtcg agaccgcacc catgcctccg ccgacctaca
     8101 aggtgaagcc gagcgagatg ctgtacggcc cgtacgcgga tccggaagaa aatttggtcc
     8161 atccggcgac acagttgccg ctgttccgtg gcgcgaactt ctgcacctcc tgccatttcg
     8221 ataaggtcaa ggatgtcacg cagaaagatc tccccggaga aattctgcgc ggtacgattt
     8281 gccaggattg ccatatggag ccatccaccg ggagttcgac gtcgagacgt ggtgccatga
     8341 cccgctcaat cggacggcat tggtttcgcg gcgtcgtcat tccaggcacg ctcctgaaga
     8401 atcgcaatct gcaggcggaa tggatgccgc gtattgatat cgaagcgacg aaggcggcaa
     8461 caggggtaga agggacggtg ttggtgaagg tcggcagcct gccgcatagt ttccctgatg
     8521 gcgacccggt gttgaagcaa ttcttcctct cgatcacgat caaggatgcg caggggcaca
     8581 tcctggcaga agaaactaaa catttcgggt tgccgtacga gaaaatttta cgcggtccga
     8641 ttcccgaccc cttcattaag ggcggcaaca ccaggcgagt gcccttttcg cagaccctgc
     8701 cggctggagg agaagaggcg tcgaccattg aagtcgtgct caactacgcg ttgatcccgg
     8761 agcctgaacc tgaactgaaa gccaaatatc tggccacgct gcccaccgac caggagcgtg
     8821 cggccgccaa gaaggtcatc gaggaatata ttcagcctcg catgctgacc tatcgaacca
     8881 aatcactgta agaccccctg ccggttctca cgacaacggg cagcgaggtg tgcaccatgt
     8941 gcggaggata tgcaaccggt ggagaatcga cgaggtctgt tgcggggact catcatcatg
     9001 gcattgttga tggtgattgg attggccagt tatggcgtgc agcaaggctt ggcgcaggat
     9061 gcaaaatctc aactgacaat cgaaaagtcc tttcccagtt ccggtaagtg caagcgctgc
     9121 catgagcggg tgtttgagga gtgggagaca tcgcctctct ccaaatcgat ccatacgccg
     9181 acattccgcg ctgccctgga tgcctatctg acgtctcctg ctggaaaaga taaggccctc
     9241 tgtttccgct gtcatgcgcc gcatgtgcgt gagtttcccg atcacgtgca gttatttgtc
     9301 actcaagccc aatccggtga gccctcctta gatggtgtgg cctgcgcgca atgccatctc
     9361 attaaacaag ttgatcgaac caaacagccg ccggaaccga agtatgacct cggcaataaa
     9421 accatgtatg ggccgtacaa agatttcgcg gcgaatctcg cccatcaatc gatggaatcg
     9481 gccctgttcc acaaatccga tctctgcttg aactgccatc aagtcgtgcc tgtagcggct
     9541 gatcttggaa agagtaatga cctgttaggg aattgggatc aaagcaaggc cgtgaagtcc
     9601 ggaaaggaat gccagacctg tcatatgcct gaacaggtgg gagagtcggc caatggcgaa
     9661 gccaagcgaa aggttgccaa ccatacattt cctgggcgta tcgggcagct caggcaggaa
     9721 gcggccaagt tggacgtggc gaccaaagtg gagggcgaca agacatccgt gacggtggcg
     9781 gtgcagagtc tcgtccctca caatctgccg acgacccatc ccggatgggc aagcgtggtt
     9841 ttggaactga cggtgaaagg gaaaaatctc aagacggtct tcagcgacaa gcgcgtctac
     9901 ggacggacct atgcggatgc caaaggtcag aagacggtat tcgattttga ggcggtgaag
     9961 gtccttgagg aaacggttct caagccggag gagactcgcg tcgaggcctt cagttttccg
    10021 acacccaagg acacgaaaac gttcgatgtc gaagccatcc tcagctatgc tcccgtgacc
    10081 ggcccggcga gctttctgca acgtatcgag gccgagtctt caaagggtgc gcaggatccg
    10141 gtgttccagc ccattccaat cgcgaaattc agcgagaatg ttccggttgc gaaatagctg
    10201 tctggcgttg agcgtgaaat ggggaaacgg ctgagaagcg ggacgagagc cactcaggct
    10261 tcgatccgct gagtggcgtc tcacgtgcaa cgaattattc ggagatgata ctggagcctg
    10321 gcgatttcat cttgaagatc tgaattgcgc tgtagatccc tttggcagga tggttcaaga
    10381 tcagtcgcga gttggtgata tgcgtgtcga ttagttcgta ttgcccgccg ctataataca
    10441 catcgcagtc gtcaatctca caattcttgt agacgtggtt gtccagcgac agcttggctt
    10501 tgctgaactt ctgctgatcg atcaggatgt attccggcgc gaatcccatg ttcgtcctcc
    10561 acagcccgcg cgcactataa caaactggat atccttgcgc aaaccgcctg cgtgctgccg
    10621 ttccgcctct tgctgcatga tctttcgcct tgatcagcct cgatcacccc tatagaatgg
    10681 cgccactttt cctctcgttc gcgaccgtgt cagttgtcgg gcatgcggcg gtattcctgc
    10741 tgcacaatca gtccgcgatg ggctcaagcc acggagcacc tgtgacattc atcgatcctt
    10801 acatgtgagt gagcttttca cggcgaatcg atcccgtacc acgtcagctg gattctcagt
    10861 ttcaccatct gtaacaggag gagaacactg tgagcgactc agcgattgtg acctttgctc
    10921 tggttgcggc ggtggccggt attgcctatg gcctgtatct cgccatgtgg gtgttcaagc
    10981 tgaatgccgg caacacgaag atgcaggaaa tttcgaaagc gattcaggag ggagcgggcg
    11041 cctacatgaa ccgtcaatac aagacggttg gtgtggtggc tgccgtgttg tttgtcctct
    11101 tgtggggcgc aggagctgta tccgataagt tcggcatgtt gacggcagtc ggattcctca
    11161 tcggcgccgg cgcgtcggcg ctcgccggat acgtcggaat gattatcgcg gttcgagcca
    11221 atgtgcgaac ggcacaggcg gcgcataacg gcatgaatgc tgccctgacg gtggccttcc
    11281 gcggaggtgc agtcacggga ttattgttga tcgggctggg gttgttggct atcaccagct
    11341 tctacactct cgcctctcag ttggcaggtc aggagaaagc cattcatgcg ttgctcagtc
    11401 tgggcttcgg cggcagtttg atctccgtgt ttgcccgggt cggcggcgga atctacacga
    11461 aggcggcgga tgtgggcgcg gatctcgtcg gcaaagtcga ggcagggatt ccggaggacg
    11521 acccacgtaa cccggcggtc attgccgaca acgtaggcga caatgtcggt gattgcgcgg
    11581 gtatggcggc cgacttgttt gaaacctacg cagtcacgac cgtggcagcg atggtgctgg
    11641 cgtttacgat gttcaaaggt gtcaatgcgc cgattctgta tccactcgct ttgggtggtg
    11701 tgaccatctt tgccacgatc atcggcattc tctttgtcaa agtcagtccc ggtggcgaaa
    11761 tcatgccggc gctctataag ggcttgtttg tcgccggagg cattgccgcg gtcgcatttt
    11821 tcccgatcac gtcgcagatc atggagggtg tgggaggtgt cagcggtatc agttattacc
    11881 tggcggcatt gatggggctg gcagtcacgc tggctcttgt tttcattacg gactactaca
    11941 catctaaggc gtatgcgccc gtcaaagata ttgcgaaggc cagtgaaacg gggcatgcca
    12001 cgaacattat tgccgggttg gccgtcggta tgcagtcgac agcggcgccg gtggtggtga
    12061 ttggagcggc cattctcgct agctattgga tttgcggggg ggcggcgtca ggcgggcttt
    12121 acggcgttgc ggtggcggcc gtatcgatgc tgtcgatggc cggtatcgtg gttgccattg
    12181 atgcgttcgg accgatcacg gacaacgctg gcggtatcgc cgaaatggcg catcttggca
    12241 aagaggtgcg tgacattacg gatcctcttg atgcagtcgg caatacgaca aaagccgtga
    12301 caaaagggta tgcgatcggt tctgccggtc tcgcggcagt ggtcctcttt gcagagtttg
    12361 cccgtgaagt ggctaagggt acgcaagcca gcaccttcga cctctctaac ccgtccgttc
    12421 tggtcgggct ttttctggga ggcatgctgc ccttcatttt cggcgcgctc tgcatgaaag
    12481 ccgtcggtca agcagccggt ctcgtggtgg aggaggtacg gcgtcaattc cggaccatca
    12541 aaggcatcat ggaaggaact ggcaaaccgg agtacggcac ttgcgtcgat atcgtcactc
    12601 aggcggctat tgccaagatg atgattcccg gcttgattcc agtcgtggct ccgataattg
    12661 tcggcgtgat tctaggaccg caagcgctgg ggggcgtgct ggtcgggagc atcgtcacgg
    12721 gactgttcgt cgcgatttcc atgacgagtg gagggggtgc ttgggacaat gccaagaagt
    12781 atatcgagga gcagggattg aaaggcacgg atacgcataa ggctgcggtg actggtgata
    12841 cggtcggcga tccctataag gacaccgctg gcccggcnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnncgg atgaactgga ggtgctccga tggagaaaca ggagaggaga
    13021 ccggaatcca gaaaagaatc acacgccaaa gaagaggtga agccgaaccc gaaggtagtc
    13081 gaatcgggca agaagatgaa agaggacatc gataaattgg tcgatgaaat cgatgatgtc
    13141 ctggaaaaaa atgctgaaga gtttgtgaag aactatgtgc aaaaaggagg ggaatagtcc
    13201 cctccttttt tatgccacct tccggtgcgc gctcctcgcc ttcctgggcc ctccgtccgc
    13261 ccaccagagc gatatcaaca cctcgaatcg ccatgctatc gtcctcccca tgccccgttt
    13321 gacaaagatg gggtgagtcg ataccattcc ccttatttct cagtaatttc tgaagcgttc
    13381 agctctaagc agaggagtca tgctcgaaaa actctgggtg ttcaggccgt ttgatacggt
    13441 cctacagaac agtctcgttc gaacgctgtc gatatcgccg gtgactgcat cgatcttgct
    13501 ggcccgcgga gtcacgactg ccgaccaggc gagccgttgg ctgtccagcc cgcaggatgg
    13561 gctccacgat ccatttttga ttcccgacat ggaggctgct gtcgagcgac tccatgtggc
    13621 cctcgcccgt gaggaacgtg tgtgtttcta tggggactac gatgtggacg gcgtatcggc
    13681 caccagcctg tacctgtcgt tctatcgagg attgggaggg aatgcctgcg gatacattcc
    13741 ccaccgggta cgcgaggggt atggcctgaa cgagggggcc gtcaggaagc tggcacagga
    13801 aggcgtgacg ttactcgtca cttcagactg cggcacgacg tcgcatcatg aaattgccgt
    13861 cgccaaagaa cttggcatgg atgtggtggt gacagaccac catcaaaccg atgcgacgat
    13921 gcccgcagcc cttgctgtgc tcaatccgca tcgacgcgac gccatctatc catttaaggg
    13981 gctctgttcc gcaggcttgg cctataaggt ggtcacggcc taccagcgac ggtatggctc
    14041 gggcgaggtc gatccggaat cctgcctcga cttggtcgcg ctggcgacgg tggccgatat
    14101 cgtgccgctt caggatgaga atcggattct ggtgcgcgag ggactgattc agatcaatcg
    14161 tggtgcccgc tgcggaatcc gtgccttgaa acaggtggcc ggcatcacaa aagcttgtac
    14221 cagcgaaacg atcggatttc gactggggcc acgattgaat gctgcgggtc ggctggatca
    14281 tgccatgctg tgtgtgcaac tgctgactac cgaatccgat cgagaagccc aacagattgc
    14341 agaacagctc gaacaactga atcgacagcg tcagcaaatc gaggaagaga ttacggcgca
    14401 ggcgggagcc tctctacagc aggatgaacc ggcggctgcc atcgtgctgg gctcgcgtca
    14461 ctggcatctg ggtgtcgtcg ggattgtcgc cgcaagatta gtggatcgct tccatcgccc
    14521 gagcatcgtg atcgctgtgg atgagaaggg cctagggaaa ggctcggcca gaaccgtgga
    14581 gggatttgat ctgtatcaag gattgtcggc atgccgggat ctgttggagg cctttggcgg
    14641 ccatccgagc gcggcgggtt tgactatcca agaatcgcga ttggaagagt ttcgacggcg
    14701 gttttgtgag gtggcggcac aatgggcgac cgccagtctg gtcagaccga ccttgcacgt
    14761 cgatgcggag gtgaagctca cggaagtcaa tttcgacttg atccaggaac tcgaatcgtt
    14821 gcatccattt ggcgcgggca atcccgaacc gacattggcc gttcgtggtc tcgatgtcgt
    14881 cgaagcccgg gtcgtcggtg agaaacatct gaagttgcgc gtccggcaag gccgctcgtt
    14941 tatttttgac agcatcggct ttcgcatggg ctcatttgaa gagctagggt tgaagacggg
    15001 gaggccggtc gatctggcct ttagccccga gcggaatcat tggaacgggt atgaccgcgt
    15061 ccaactgcgc atcaaggcgc tgcgcatgag cggcgaggtg tcctaagcat gccccacgag
    15121 acagtgacag aactcgatca attgatcgag cgggtgaaga gctataacgt cgaggcggac
    15181 ctggacctcg tacgccgggc ctatgatttt tcggccaagg cccatgaagg gcagatgcgt
    15241 cgatccggcg agccgtactt tcgccatccc ctcgcggtgg cgggcctgtt gacccatctg
    15301 aagacggacg tgaccgccat tgtggccggc ctcctgcacg acacgctgga agataccctc
    15361 gcgactccgc tcgaactaga gcagcggttt ggaaaagacg tcgtccatct cgtcgacggc
    15421 gtcaccaaga tcggcaagat cacctttcgg aactacgagg aaaagcaggc ggaaaatttc
    15481 cggaaaatgg tgttgtcgat ggcggacgat atccgcgtcg tcctcatcaa gttggcggac
    15541 cggctccata acatgcggac cctcgagtat ctcagcgaag gaaaacgacg gcagattgcg
    15601 caggagacgc tggagattta tgcccctctc gccaatcgct tgggaatcgg gtggattaag
    15661 aacgaacttg aagacctctg tttgaagcac ctcaagccgg atgtctatga aatgctgcgg
    15721 gtgcgcgtgg ccaagcggga cgaagaccgg cagcagtaca ttcatgaagt gatcgacatg
    15781 gtgaagaagg ccatggcgga agccggcttg tatggggagg tgtccggtcg cccgaagcat
    15841 ctctatggca tctaccaaaa aatggaaaag cagtcgatct cgtttgagga ggtctacgac
    15901 ctcgcagcgt tacgcatcat caccgatatc aaaatgaatt gttacgcctt gctcggcgtg
    15961 atccattcgc tgtggcgccc cttgccaggc cggtttaaag attacatcgc cattccgaaa
    16021 tccaatatgt atcagtcgtt gcataccacg gttgcaggac cgaagggcga gcatgtggag
    16081 tttcaaatac gcaccgagga gatgcatcga gtttctgagc agggcatcgc cgcgcattgg
    16141 aaatacaaag agcacggacg tatcgacgag aaggacggaa aggtcttcag ctggctacgg
    16201 cagttcgtgg aatggaatca ggacctgccg gacaatcgcc agtttatgga ttcggtcaag
    16261 ctcgatctct tccacgacgt agtgtacgtg tttaccccgc agggcatggt gaaggaactg
    16321 ccgaaggggt ccacgccggt cgacttcgcc tattccgttc atactgagat cggggaccat
    16381 tgtgtagggg ccaaggtcaa cggcaagatt gtcccgctca aacacatgat ggaaagcggg
    16441 gacatggtgg agattctcac cgcggccaat caaacacccc accgggattg gctgaagttt
    16501 gtccgcacct cccgcgcgaa gacgaagatc aagcattgga tcaaggcaga ggaacagtct
    16561 cgcagcatcg aaatcggcaa acgactgcta gaggccgaat tccggcggca tggactggct
    16621 cccgctcaga tgatgcgctc agagcaactt ctcgcggtcg ccaagcaggt tggatatgaa
    16681 actgcggaag aacttgccgc cgctgtgggt ttcggacacg tgccgacttc tcaagtcatg
    16741 agcaaaattg caccgccctt gccgggagaa gctcagaccg tggcgcctga ggctccagtg
    16801 ccgcacaagc cggtgatcgg tcgcacggat gatacggggg tccaggtcaa gggggctcgc
    16861 gacctcctga tgcaactttc acgctgctgc aaccccgttc ccggcgaccg gattttgggt
    16921 tacatcacca gggggcgcgg gctcactatt catacgattg attgtccgaa cctcgaagcg
    16981 ctggattacg ataaaaaccg gctggtcgaa gtcgagtggg atcaatcgac gcctagcacg
    17041 cattccgtga aagtgtcagt gatcgcagtg gacaagacgg gagtgttggc gcatgtctcg
    17101 acagccatct cggagtgcca ggcaaatatt agtcgagcag aaattacgac ccgtgaagat
    17161 cggaaggcga tgctggactt cattgttgaa gtggcggaca cggcgcactt gtcgagggtg
    17221 tttaaagcga ttgagaaggt ggaaggcgtc atcaccgtgc gccggatgcg ttcatggcag
    17281 gagcgggcgt agcctgaaag gtctctctgt gcgatccttc cctcggtggg tgatgtccgc
    17341 atgactctgg gaactctcgt cagttccatc ctggccattc tggcttgtgc ggctggggta
    17401 ttggtcgccc tgacctacgt gtttactcgc cgcgggaagg ctgttcccgt tctgcccttt
    17461 tatgccggtg gtgtgggcgt ctttttcgtt gtggcatcac tgatcggcca cagcggtctt
    17521 gggacttctg ttcctcactt cgttcagtcc tgcctgaatg tcgcagcctg gcttggcgca
    17581 gcgttcttct tcttcacgat cctcgatgtg ctcatcatcg gcgaatggct gatcgagcgg
    17641 gggcagcggt acatccctga tatcgtccgt cagttgctca tcggcgcgga agtcgtggcg
    17701 gccggtgtgt tgattctctg gttggtcatg gggatcaacc tcgtggctct cgtggcgttg
    17761 cccacggtag cggcggcgat ggtcggcgtg gcgctaaagg atacgttgac gagatttttc
    17821 tccgggattg aactaggaaa aatcgtcaag gtgggggatt gggtcacagt gcttgatcga
    17881 gaaggggtgg tgactcatat cggtatggag catgtcacct tattgacccg tgcccatgat
    17941 gtggtgtcgt tgccaaacga cctggtcatt caaagcggcg tgaccaattt caccagaccg
    18001 acgacgacgc atttttgcaa cgtgtttgtc gatgccgcgt atcgaacccc gcctgaacag
    18061 gtttgcgcga cgttgctcga gactgcgagt tcagtctcgg gggtgttgcc ggatcccaaa
    18121 cccagcgctt ttgtgactgc atttaatgaa tcggccatcc aataccgtat caagtttccg
    18181 attggcgatt tctcgcaacg ggattccatc gaaagtgcca tgcgtaccta catctggcat
    18241 gctttcgctc gtacgggcat tgagattccg tttccgcagc gcgtggtgca tcaatctgct
    18301 aatggacagg atgcggctca agatcgttac gccattgagc ggatcatggc gaaactgaca
    18361 gcggtcgatt ttttggccac gcttgatacg aagcaaatcg aagtgctggc ggaaggtgcg
    18421 agaggggagt tgtatttgcc tggcgagcga attgttcggc aaggagatcc tggagaagtg
    18481 ctgtacgtga ttgtctctgg gacagcggat gtccgtttag aacagggtgg actgactacg
    18541 acggtgtcga cactggaacc aggaaagttt ttcggtgaga tgtctttatt gaccggtgag
    18601 ccgcggtcgg ccacagtggt ggctgtcacg caactgtcgg tcattgcggt gagcaagtcg
    18661 gctttgctgc aggtggttca ggaggaccgg cggttgatcg aacgaatcgg cgaggtcgtc
    18721 gcgcgtcgac aggccgttac ggcggcagcg aaggcgcagc tttcgagaga tgcggcggcc
    18781 ttgtcagtcg tcacgcatac acgttctctc gtggaacgta ttcagagttt tttctgggga
    18841 acgcgtaagt cgacatagcg agcggccgga gtccgatcag ggttgtttaa agctgagttt
    18901 gacgcctcgc tgaagctgaa gcgcggccgc ccgtcccgct ccgagttcaa gcacgtagag
    18961 cgccccctcc tccgaatgat actgcggaca cccgtcgtct tgtctggtgc agatgggcgt
    19021 gttgcgttcg atatgaacga tggttttttt cgcatccatc cagatgatat cgaggggaat
    19081 tttggtgttt ttcatccaga acgtccaagg ttgggcgtca ccgaagatga acaacatgcc
    19141 tcgatcgtct gcgaggcgct cacgaaacat caatccctgt gcacgcttca gcgccgtgtc
    19201 cgctaattct gcctgaatca cggtaccatt cggcattttg accgcaataa gagtttcagt
    19261 cggtagatct gcggcccagc cagaccaggg gctcgctact atcgcgaggc tccacaagca
    19321 actggtcagt cggctcagcc atgtcgagtg aggtgtttgg ctggaatgat ccatcgattg
    19381 tgtcatggcc gcatcctaac gggctgtttg tgagtcgtca aggcagaggt gatgcgagtt
    19441 ctggagatag agatgccgcc ttgcaattcc gtcggcccct atgccatcct cggcatatca
    19501 atttgacttc gagggaggct gttcatgaaa gaaacgcgac gtgtggtgta catgcgaggc
    19561 gtgtttgtct gtccgaagtg ccgccagtct tatgtgcagg aaaagtggat cgaagaattt
    19621 agaatccggt gccacaagtg cgactatcgc gggacattga cccaggtgtc agttgaagag
    19681 catatggacg aggaagtaat tcgtcgctag gacgcggctg ttccgcatgg tgaatttctt
    19741 atctcttgtc ttctaatcgg aagacataga ttctcgccag gccgttcaag gcctggcccc
    19801 cccgatacca gtcttgctct cagaatcacc aagaggtcca tcatggttca gatgtggcga
    19861 ctgctgcttt tgtgcgcgac tagtattctg atagggccta ctatcggctc atcggccgat
    19921 agtccaccca tcacccaagt gctagtggcc tatcactcac tctccgggaa taccgagcga
    19981 atggcggcag cggttgctga gggggctcga acggtgcctc acacggaggt catcgttcgg
    20041 cgtataggaa gcgtgacggc tgaggagtta tttgcggcag acgccatcgt tctgggctct
    20101 cccgtctatt ggtccaatat ggctggggaa gttaaagcgt ttatcgatga ctggcagttc
    20161 aagttcggcg tgtttccgga gtttaaattg aagaataagg taggagccgc cttcgcgacc
    20221 ggtggtcaga tttccagcgg gaaagaactc accatgctct ccattcttgc cgcgatgctc
    20281 ggaaattata tgatcactgt cagcggcggc ggggcatttg gcgcttctgc cacaacagaa
    20341 ggcaatagcc caggaattga tgagcaggag ctcggcagtg ctcgggaatt aggccggcgg
    20401 gtagctgacg ttgccggggc agtcaagcgc gggtttgcat tgcaagaacg tggcgtgcgc
    20461 tgaagcctac aatgaaggac ctgtctggga gattaggatg acaccggatt gcggcaggca
    20521 ttctccaggt gacttttcgc gagggcgact aattcgtgga tatttttggc gcaacgacca
    20581 cagcatccat tttcccgtag accgaattca gcaataattt gtctcgtggt caggtgcccc
    20641 tggcgacccg cttcccgcac atctgattcc gttaagccct tacataagca gacatacatg
    20701 agtggcgtct cccactgatg atcaattgtc tcggccgggc gatgcgaata gcttctcgat
    20761 actgataacc gttctcaact gtaaagagta tagcggtcgc aatgattcct gtcaacctca
    20821 agacagattg cagacggaac gatgccgtcg acccaggcga ctatatgaac gagtgacgta
    20881 aacagggggc taaagctgaa gcttcaggcg ttatgcggta tgtggggaaa aggtcttctg
    20941 ctcattcgga aaattgcttg aagcgacaac actctagtac ggaaacgttc gtggaagagt
    21001 cgagttaaac gatcgtgata ttagttgagg tgaggaaaga tatgaactga tcatgcaggc
    21061 tgtcgataaa cgtacaaagg ccggaagttc tttcgaactt ccggcctttg ttgtgctgcg
    21121 gtccttcact ggcgagagag gttgccctct cccgccagtg tgagtcttac agccagttca
    21181 cgcgctcggc cggccgaatg tagatcggct cttcgacctg a
//
LOCUS       DAOQEW010000048        20871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_629_length_20871_cov_8.910165, whole genome shotgun sequence.
ACCESSION   DAOQEW010000048 DAOQEW010000000
VERSION     DAOQEW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20871
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_20871_cov_8.910165"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLS35_07340"
     CDS             <1..866
                     /locus_tag="PLS35_07340"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNI18509.1"
                     /translation="HGFRPLCLGRFRDAWIVASESCAFDLLDAEYVREIEPGELVVLD
                     HRGVTSYKPFPPTQPAMCVFEYVYFARPDSRIFGGSAVYSIRKAFGRQLAQESAVDAD
                     IVIPVPDSGVPAALGYSEGSGVPFESGLIRNHYVGRTFIEPEQSIRHFGVKVKLNAVP
                     EVLAGKRVVVVDDSLVRGTTSRKIVKMLRHAGAKEVHMRISSPPIVSPCFYGIDTPTK
                     KELIASSHTTDEIRKYITADSLAYLSLDGMVKAAPGTPSQYCNACFTERYPISFTRAE
                     ELQLGLFEPPG"
     gene            1415..1732
                     /locus_tag="PLS35_07345"
     CDS             1415..1732
                     /locus_tag="PLS35_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNI18510.1"
                     /translation="MPATVPVLSERTIDFSQMFPHQNSDVRITNQGGESLLFNVRTGH
                     FHRLNALGSAVWLQCTGTQSVARILSTISETFDVTATQAQSELLDVLVQLQDDGFLRI
                     EKR"
     gene            2017..3165
                     /locus_tag="PLS35_07350"
     CDS             2017..3165
                     /locus_tag="PLS35_07350"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNI18511.1"
                     /translation="MSEFVRVEITDLVCDGINWDLLWQLSKAHGVAPLVYRNLAAICP
                     AAVPSAIHEAYRRQNQSTTLMNTVLAKELVALLDALAAKGVMAIPFHGVTLAQVAYGD
                     LSMRECEDIELIVEHGAVSQARKVLWSHGYQLLGADNDAEADPREGHHVFLKRNGMAM
                     VKLQWGLAGYHSNFRLDRHALWGRLKPVGLPTKAVMGLCPEDLLILLCVHGTLHNWKQ
                     LKWVCDIAEFVRRKPALDWSRILFQASEWRCRRMVLLGLAMAQNLFDIVLPRTVSHEI
                     ESDTDLPVLVRRMPQQLLKHPDQGIDEDCADAVSLTVKDSVWARWKLGFILCRVDAEV
                     IHRPLPWFRFQRQLRLMSACMRPLLRSVPKEIVPVRVRHMVVGWLQSS"
     gene            3354..3896
                     /locus_tag="PLS35_07355"
     CDS             3354..3896
                     /locus_tag="PLS35_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNI18512.1"
                     /translation="MKRVFIVTALVLLPLWAAHIAMAAGFFKVGEPAPAFALSSITGD
                     AVSLEQLKGKVVVLGLFHICDPCMMQGTNLQKVHEVLAGKNVAVVGINSSGNSKRDVG
                     EFLSAFPVKVTYPYLLDPNKVTDKLYGGGKFIPNVYVIDQRGVIRWQRVGNMELAGAE
                     VIIQEVEKLLASAPAGSGPM"
     gene            3914..4153
                     /locus_tag="PLS35_07360"
     CDS             3914..4153
                     /locus_tag="PLS35_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18513.1"
                     /translation="MDEWEEGKLMFLEVVKGIDQGVEVVIPTKPTNSMFLISLTKGAN
                     RKFITVSEDDIIDLPTEAGIRTKVTKTLQDAVATL"
     gene            4177..4773
                     /locus_tag="PLS35_07365"
     CDS             4177..4773
                     /locus_tag="PLS35_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18514.1"
                     /translation="MKQLLPGIWQWSWFSDEKRLDFNGLFLTVGEHRIVIDPPPMTAE
                     ASTLIRRQGALDYIIVTNRDHLREAVAYQAEFRCQLQIPEADASQMDVKPTKTYKDGE
                     LLPGGIWAIQLKDQKSPGESALYIQQGKGVLIVGDALIGKPAGALSLLPSERYGDVAK
                     AKEGLRRLLKYNFDSILVGDGASIVFGAKQAVEQVLQA"
     gene            4780..5604
                     /locus_tag="PLS35_07370"
     CDS             4780..5604
                     /locus_tag="PLS35_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18515.1"
                     /translation="MPLRNGLSEELNRQGNEHFARGHYTDAYACYAKALECDRLTGDH
                     RALSATLGNLGNICAVSGRRESAQSYYQEVLELQKVLGDDKGIGTTLANLGNLRADAG
                     EWDRARAYYLEALDIMTRVHDELGKAVLFSDLGLVARETGQCDDALRYYEQSLVLMRR
                     LNNQGGVADAWRMMGRTFAIQKRYDDAIACCQTSQSIAERSRDELRAGGARYVLAQCY
                     EDLGQLQAAVDLLEQVVRMDRKYALPKLTENTARLASLRARLEAEDPSPQPRRSRA"
     gene            5601..6032
                     /locus_tag="PLS35_07375"
     CDS             5601..6032
                     /locus_tag="PLS35_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18516.1"
                     /translation="MSTVALPAWEAARAKLRTNIPQFYELEPGGPLVLDLGDDGWILE
                     LRSDGQFLCQHGISMDDVKSLMCDGTTEDLGTDEVAKQAKYFLGPAVSKKRPALLKAG
                     FAEQTEMNDEYVAITFLKAVNFERYEEVLGSVRWCQNLFKT"
     gene            7294..7896
                     /locus_tag="PLS35_07380"
     CDS             7294..7896
                     /locus_tag="PLS35_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18517.1"
                     /translation="MNVKEEGAAESDGSKGRIFAGGIGADSQGKSGFMLRVYDAETGR
                     FLWEGQLNLLKVNEEGPSKVKAVIAPKPAVVIPASDRPPQVFQTFFSVRAVNPLTGRL
                     VWQDQFTPGTRKRPGSAGVSCKRCSVSPVNEPIGHIFDLVVRTYDRTSGLLLWEDSFE
                     EIDRIDESDSAPETSAYPQAIPFWHPGVADKTPMYKVTLR"
     gene            complement(7900..9339)
                     /locus_tag="PLS35_07385"
     CDS             complement(7900..9339)
                     /locus_tag="PLS35_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18518.1"
                     /translation="MYRRNIKHILGPLAILIAIFIFYQSWLKPHYFHEMPAPPPQVIT
                     EEVAGSKGPVAPPQQAVLPEMKRSRTIDPVSIPVPRHSELLGTIHDELEKHNIALAQT
                     KLEELPSSILSESATKRHVAILWNNLGILQENAGGTETSVKAFKKAVMLDPQNPVAHL
                     NLAHAYWGLRDPALTEEFLKKVLTLSPDEPFPHVALADLLQEKDRLSEAAKHLAQAMD
                     RIKKDPGLQSYVKVVTAKVHRAEKVEEKFSTRNSVHFTVKYDGSEDPETWTTVLDILE
                     AAYRDIGQKFNFFPSKPIMVVLHTKTQFQGSTGSPVWADGLFDPVLGRIQIPSQGATT
                     DRAWLTRVLRHEFVHALIHEELGATGGAIPTWLNEGLAMQLANDSWQEVANMPPGEHI
                     LIPLAALEEGWENLSAEKVGAAYMEANAATHYLIERFGMHKVHELLLHLKARQTIAAA
                     MQDRLLLSYDHFQQQWVESMGATLSTPKS"
     gene            9561..12044
                     /gene="recG"
                     /locus_tag="PLS35_07390"
     CDS             9561..12044
                     /gene="recG"
                     /locus_tag="PLS35_07390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNI18519.1"
                     /translation="MARPIEFACRDAYAHLPTVKNLDRFVSEQVIGALGERLHPRALE
                     VELLSLRNLFVDFHAGLTAAEQQHRLTQALQLLRRLQEESPMVHPPADLLEGGELVHT
                     PANIHSRQLSVESSIQFAKGVGPKRTLLLKRLGIQTIEEALWTLPWRYDDRSTITPIA
                     KLMLAATVCICGVITRTEASRARSRRLSILDVVVQDVTGTVHAVFFNQPYLEDVLKEG
                     ARVMMAGRVVAGRRGWTDLRLEPTQFEILSGQEDELLHVGRIVPIYHETKGWTSRQMR
                     VLIQGVLAGHGAELVDVLPLSIRARHRLPPIGEAIQQVHFPPPQTDLAALDRGVTAAH
                     RRLAFEELFLLQTAMVLRQQQVKDEEKPFRFSPHVPELKKLSQILPFSLTAAQERVWR
                     EIQADMATSRPMNRLVQGDVGCGKTVVALHALVMACGSGCQTALMAPTEILAEQHYLN
                     LRPLLEAVGFKAVLLTSGGKAKDRQAVFTQLASGEAQVAIGTHALIQKNVAFDKLGLV
                     VVDEQHKFGVLQRKSLLDKGYRPDVLVMTATPIPRTLAMTVYGDLDVSVIDMLPPGRK
                     PVRTMLYSEGQRHKTWQLVSDELKAGRQAYIIYPLVEESEKIDLKAAIQGAEQLQRDI
                     FPQAQIGLLHGKMPSSEKERTMAAFKAGTIQILVATTVIEVGVDVSNATVMVIEHAER
                     FGLAQLHQLRGRVGRGAHQSLCILMASYLPRESRPKVSAEGKPEANASNAQQRLSALV
                     KSHDGFVIAEEDLRIRGPGEFLGLRQWGLPEFRAANLIRDTHLLELARQEAFAMVTQD
                     PGLALPQHQAVKAAMFRRWKGKLSLGDVS"
     gene            12089..12415
                     /locus_tag="PLS35_07395"
     CDS             12089..12415
                     /locus_tag="PLS35_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18520.1"
                     /translation="MGMLQRLSHDLRAGWVTLRHGTAKAATRALEEGELLGYRLELRK
                     VEQQLDDLHVDIGERTIELHDRGEAPDRIVADSEVARLMKQVQALQDERTKLLLEMND
                     ITVEEA"
     gene            12424..13395
                     /locus_tag="PLS35_07400"
     CDS             12424..13395
                     /locus_tag="PLS35_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNI18521.1"
                     /translation="MAGASTGSSRILAGIDIGTLTCRLEIAEVTSSGQVKKLRSDRRI
                     LRLGQGVDRDRMLRTDAMARVVTTLKEWRQSIDTYQVEATSVVATSAVRDAGNRQDFL
                     DLIAREAGLQVEIISGEEEARRTTLGIRSGLPEGVSDMLAVDIGGGSTECILDRPGQP
                     LIVRSIDIGVVRLSERVLQSDPPTAEELQQAHDWIRTETGAAIADMPLPVGLTFVGTA
                     GTITALAAMEQQLPVYEAAKIHNYVLTLEAVKRLEAQLLGRSKQQREGLPGLERGRED
                     VIVAGAIILSTMMETLGMAKVLVSDLGLREGVLLHLASRLNGGSSLR"
     gene            complement(13450..14943)
                     /locus_tag="PLS35_07405"
     CDS             complement(13450..14943)
                     /locus_tag="PLS35_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18522.1"
                     /translation="MKAAAAIQEPLNLSGDILSLLAWRIPDLVAYQHAFDEWWLAPLD
                     DDFPLVRLNAVGIEMLTSMNGHITVGALVEKYGNKICGPDGQPGSWHLAHWSIPNYSL
                     CYFGTEPPGGHRHKAKWDLLLQQIRESWSGQQDFEGEEHLEDFHVHELKESDLEDGHF
                     DLIETTVSHLFREPCEALGGTTYGRLLMRQLRRLGWFKPKPKVIVEIGGGLGYVAREL
                     GQELLPFEKQGIQYISLDVTRPFLQLQTKRARAGGWTGTGTHANGEWLPFKDNSVDLI
                     IDNENMADMTPVKLSREELTSGRGETAQHQEALDWIRRLRLPLDKELPEEVIFNLGPM
                     RFVAEVWRVLKPGGRAFLTEFGIEEGWPAAVKLPHHTEYEVQYSHLRQAVRWLGFQER
                     YLSLPQFLQLKPDTKVLCTGAAYTIQRFCQGLGQHFSVRAYTESELTKTLGDTLPKLQ
                     GCHYHDIADPAWFGLIDFKVLLLEKPGGMPQATFTEQKSGLRWYSQR"
     gene            complement(15025..15399)
                     /locus_tag="PLS35_07410"
     CDS             complement(15025..15399)
                     /locus_tag="PLS35_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNI18523.1"
                     /translation="MSWRTLSLSLTFAFLMAWPSSATPPAVHVLMDSGSPYYVPAAAT
                     VTAGGAIRWDNPTPTHHTVTHDGCFDESGRCAFDSGAVEPGGTYTIPSLPPGRYPYQC
                     RIHPIMRGIIIVTDSPLLPSQT"
     gene            15696..>15883
                     /locus_tag="PLS35_07415"
     CDS             15696..>15883
                     /locus_tag="PLS35_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18524.1"
                     /translation="MAKKTMKKKSSAVKVARPKKGAAFKASKPKKVVKAKKAAPKKPV
                     KAKKAAQPKVTAKKPVKKT"
     assembly_gap    15884..15983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15984..16164
                     /locus_tag="PLS35_07420"
     CDS             <15984..16164
                     /locus_tag="PLS35_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18525.1"
                     /translation="QLDLEDDEEDMAEDLDDVSEEEMEEEIEEELGLDNEDDDVDYLE
                     KSEDLLDDADEYRNN"
     gene            16376..16828
                     /locus_tag="PLS35_07425"
     CDS             16376..16828
                     /locus_tag="PLS35_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18526.1"
                     /translation="MSHFLFIAGALRERTSPESAELQLGAGIWGLCTSLIRDNLQKFL
                     TPESHGLVYVLKEGIRAEFSIVSPVQPFQALDELLKDELRTEARYGFVRVTPVRRWEG
                     NAPDCQQLLQRVLGIEEQSELSRRLGLGMHRLSQEEYRAIVDGLGAEH"
     gene            complement(16856..17407)
                     /locus_tag="PLS35_07430"
     CDS             complement(16856..17407)
                     /locus_tag="PLS35_07430"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18527.1"
                     /translation="MLAAIQSNQLGVFEVLLLRWDHQPTPSPIMLQWQGGNVKPGQTN
                     LGSMVLALQYALEHTPTVNHTGTISVIGISYAATGTDGPSAGGVMTVGFAALLKGEQI
                     RRGIAMTGTISKDGTIGPVGSIPDKIRAAAREGYRTILIPQGQRDDPRWNLTRLAWDL
                     NVEIKEVATVDEAYHLMTGGTLD"
     gene            17625..18719
                     /locus_tag="PLS35_07435"
     CDS             17625..18719
                     /locus_tag="PLS35_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNI18528.1"
                     /translation="MVNWPRQLHRISAWILGILWGTVMLPGLLFAECTSETGGKTASA
                     VFTLHLTGTCTEAEREARAVPAKDLMRALAAGKGIDLAGVVIQGDLVLDELPAQKVDA
                     VQGLSLEDRRVLEGLNDEEVHVIRGPFVIKHSRVKGQIVNRLKRGFLLITGPVVLVHS
                     GFDGLVDLSRTVFLGLVDGSNAKFEKESYFVQDRFTQGAMFSDTRFGSHARFHRSMFS
                     GPAIFRGADFPGLTEFLEVVFEQDANFARTAFHMGTGFSGAHCRGKCDFSSTLFEREA
                     FFLFARFDRAVTFASAKFSSQADFSDASFKEADDLAKATFVRPPVMIRTARVVSTVPV
                     APEAGPFSQVVTIGLFVAALGILIYILKAK"
     gene            18815..19594
                     /locus_tag="PLS35_07440"
     CDS             18815..19594
                     /locus_tag="PLS35_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNI18529.1"
                     /translation="MDQPELPYQVKIENFEGPLDLLLHLIKKNEINIYDIPIALIAQQ
                     YLDYLSVMKELNLAVAGEFLVMAATLLHIKSRMLLPTDEVAVDEEDGPDPREELVRRL
                     LEYKQFKEAASQLDYKERLWRDTFGREPGPPIEIAKDESLLDEISLFDLVDALQGVLS
                     RHPGKRLVEIIPDNLTVRTRMSAIIEALEVHESMPFTALFEESSHRLVVIVTFLALLE
                     LIRIRVVRVFQSETFGAILVSRAFTAVAEGDLVEESEWKNL"
     gene            19579..20502
                     /gene="scpB"
                     /locus_tag="PLS35_07445"
     CDS             19579..20502
                     /gene="scpB"
                     /locus_tag="PLS35_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNI18530.1"
                     /translation="MEEPMSLSMEDAVEQPAEVAEMEAPVSEEPTAEPEVEAHAGEGD
                     VAEMAAPTPASDPALSDVRALKGILEALLFVTPDPIPVTRFLALLGAVTKQEVDQALA
                     SLSHDYEQEGRGLQLAEVAGGYRIVTKAEFAPWLKRLEKVKAPSKLSRSALESLAIIA
                     YKQPIVRGEVEQIRGVETSGVIRTLLERKLVRIVGRKEEPGRPIMYGTTKVFLEHFGL
                     RDLSQLPPLREFKELGESEQAMLPIDESEAVGPEALSPAADTVFEDNVEASNGSVELI
                     ELNGSSEALPEPSVELSSDGELMAAEVAEES"
     gene            complement(20665..>20871)
                     /locus_tag="PLS35_07450"
     CDS             complement(20665..>20871)
                     /locus_tag="PLS35_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18531.1"
                     /translation="GEVCSMYMEWIHLNQNSRGGHIFGTDPSYGGNPTAQNWYAPSYS
                     FYDNARYVTLSRIFGAGFIPIGCR"
BASE COUNT         4497 a         5253 c         6292 g         4729 t
ORIGIN      
        1 cgcatggatt ccgtccgctc tgtttggggc gctttcgcga tgcctggatt gtcgcctccg
       61 aaagctgtgc gtttgatttg ctcgacgcgg aatatgtgcg ggaaatcgaa ccgggagaat
      121 tggtagtcct ggatcatcgg ggagtgacga gttataagcc ttttccgccg actcagcctg
      181 ctatgtgtgt gtttgaatat gtctattttg cgcgccccga tagtcgtatt tttggtggga
      241 gcgcggtcta ttcgatccga aaagccttcg gccggcaatt agcccaggaa tcagctgtgg
      301 acgcggatat tgtgattccc gtgccagatt cgggagtgcc ggcggcgtta gggtattccg
      361 aaggttcagg cgttccattt gaaagcgggc tcattcggaa tcactatgtg gggcgtacgt
      421 tcatcgaacc ggagcaatcg attcgccatt tcggggtcaa agtcaaactc aatgctgttc
      481 cagaagtcct tgcaggaaag cgtgtcgttg tggtggatga ttcgttggtg cgcgggacga
      541 cgagtcggaa aatcgtcaaa atgctgcgcc atgctggggc caaagaagtg cacatgcgga
      601 ttagttctcc tccgatcgtg tccccgtgct tctatggcat tgatacgccg acaaagaaag
      661 agttgattgc ctcgagtcat accaccgacg aaatacggaa atatattacc gctgatagtc
      721 tcgcctattt gagtctcgat ggcatggtca aggcagcgcc tgggacacct agccaatatt
      781 gcaatgcctg ctttactgaa cgatatccta tttcttttac ccgagccgag gaacttcaac
      841 tcgggttgtt cgagccgcca ggctgacggt aaaaatcttg tcgcgtgtta cgctcgcccc
      901 tgaggtcaca gtagccttat gcgcatgcat agagcagcac tcccgggttc atgaaggtga
      961 attcatcggg tctgattcct tttcgcaagt aattacagca gcgctgacgt ttggtccgtc
     1021 gatcgtcatc cttcgcactg ggctttccct tcccgaatac cttttccatc gcattgggtc
     1081 cgctgtgggt tattgactgg tctcacgaga ccaatgtgcc tacgggccaa agagtctctc
     1141 caaagatccc ggttctcatc agctcaaagg aaggggcatt gtcgtccctc tctatcggac
     1201 tgccataaaa gctctgtgtt cccctccaaa gagatgttgc accaacctca ctgggaatag
     1261 agaaaccgcc ctgctacggc tacctttgcc tacatgccgc atcggcatgc tacatccggg
     1321 ttcggcgcac tagattccca aattcgcgtc atggatgaac ccgcaacctt tgcactggtc
     1381 gatgtgttgc cccctctgag tcaggaggtt ctctatgccc gctacagtac ctgttttaag
     1441 tgaacgcaca atcgattttt cacaaatgtt tccgcatcaa aattctgatg tgcgaatcac
     1501 aaatcagggc ggcgaaagcc tattgtttaa tgtgcgcacg ggccatttcc atcgattgaa
     1561 tgccttggga tccgcggtat ggctgcaatg taccggcact caatcggttg cacggattct
     1621 ctccaccatt tccgaaacat ttgatgtgac agctacgcag gcccaatctg aattgctcga
     1681 tgtactcgtc cagctgcagg atgatggatt tctccgtatt gaaaaacgat gactatccat
     1741 agagcagctg gatccatctg aatgtgcaag cgaaccggtc aatcatgagt tattgtgcgc
     1801 gccgacgtcg catatccgcc gtaggtcctc actattcttc gggtaaaacg ctcagcagga
     1861 gttaggattc caatgggaca gcagacaaga tctacgatcg ctcgatggaa accatcacgt
     1921 cgggtagagg cttttcgcaa gtcgaagccg ccacgtccag gaatctccgc aatgagtgac
     1981 gagggccgct tgctcgtcct ctgcgcacga accgctgtga gtgaatttgt tcgagttgaa
     2041 atcacagacc tagtgtgtga cggaatcaat tgggacctat tgtggcaact gtcgaaggct
     2101 catggtgtcg caccgctggt atatcgaaat cttgctgcca tctgccctgc ggcggtcccg
     2161 tcggctattc atgaagccta ccggcggcaa aatcaatcta cgactctgat gaacaccgtg
     2221 cttgcgaagg aacttgtggc gctgcttgac gcgcttgccg cgaagggagt catggcgatt
     2281 cccttccacg gggttacatt agctcaggtt gcgtatggcg atctgagcat gcgcgagtgc
     2341 gaagacattg agctgattgt tgagcatgga gcagtttctc aagccagaaa agttctttgg
     2401 tcgcacggat accaacttct tggggctgat aatgatgctg aggccgatcc tcgtgagggt
     2461 catcacgttt tccttaagag aaacggaatg gccatggtca aactgcaatg gggccttgcc
     2521 ggctaccact ccaatttccg acttgatcgt catgcattgt ggggtcggct gaagccggtg
     2581 ggtttaccga cgaaggcggt gatggggctc tgtcctgagg acttgctgat tcttctgtgt
     2641 gtgcacggaa cgttacataa ttggaagcag ttgaaatggg tgtgcgatat cgctgaattc
     2701 gtgcgtcgga aaccggcgct tgattggagt cgaatcctgt ttcaggcaag cgaatggaga
     2761 tgcagaagga tggtccttct cggtctcgct atggcgcaga atcttttcga tattgtgctg
     2821 cctcgcacgg taagccatga gattgaatca gatacagacc tgcctgtgct cgtgcgaaga
     2881 atgcctcaac agttactcaa gcatcccgac cagggcattg atgaagattg tgcggacgcc
     2941 gtgtctctca cggtcaaaga ttccgtatgg gcgcgatgga aattaggttt cattctctgt
     3001 cgagttgacg cagaggtgat acatcgtccc cttccgtggt ttcggttcca aagacagctt
     3061 cggctaatgt cggcatgtat gaggccactt cttcgaagcg tgccgaaaga aatcgtcccc
     3121 gttcgtgtac ggcacatggt tgtgggctgg ttgcagagtt cttgatcgtc tcggtccttc
     3181 ctcagtcagg cagttgttta gtcagcctgc gccctttcct gggtaagtgg ttgctcccga
     3241 aaaaattcgc gtgctaggat gccagcatgg tcattcatga gggattatgc tgtgcatcag
     3301 cgaggaaagg cagtggcttt tcccgctggc tggccttgcg gaaggaggcg cgaatgaaac
     3361 gggtcttcat cgtaactgca ctggtgctgt tgcccctttg ggcagctcat attgccatgg
     3421 ctgctggatt cttcaaggtg ggagagccgg cgcccgcgtt cgcactctcg tcaattacgg
     3481 gagatgccgt ttctctcgag cagttaaagg gaaaggtggt cgtcctaggt ctgttccaca
     3541 tttgcgaccc ctgtatgatg caaggaacca atctgcaaaa ggttcatgag gtgctggccg
     3601 gtaagaatgt tgccgtggtt ggtattaatt catcgggcaa ctccaagcgg gacgtcggtg
     3661 aatttctgag cgcgttccct gtaaaagtca cctatccgta tctgctcgat ccgaataagg
     3721 tgacggacaa actctatggc ggcggaaaat ttattccgaa cgtgtatgta atcgatcaac
     3781 gtggcgtgat cagatggcag agggtcggca atatggagct agccggtgcc gaggtcatta
     3841 ttcaggaggt cgaaaaatta ttggcttctg cgcctgccgg ttcaggtccc atgtagcggg
     3901 agaaagagga tccatggacg aatgggaaga gggtaaattg atgtttctgg aagtcgtcaa
     3961 gggcatcgac caaggcgtgg aggtggtgat tccgacgaag cctaccaata gcatgttcct
     4021 gatctctctg acaaaaggtg caaatcgtaa gttcattact gtgtcagagg acgatatcat
     4081 cgatcttccg accgaagccg gtatccgcac gaaggtcacg aaaactctgc aggacgctgt
     4141 ggcaactttg taaaccctgc agatgaggcg aaacgcgtga agcagttact ccccggcatt
     4201 tggcaatggt cctggttctc cgacgaaaag cggcttgatt ttaacggact gtttctgaca
     4261 gtgggcgaac accggatcgt tatcgatccc cctcccatga cggccgaagc aagtacgctg
     4321 atccgaaggc aaggggcgct ggattacatt attgtgacga atcgtgatca tctccgtgag
     4381 gcggtcgcct accaggcgga gtttcgttgc caattacaga tacccgaggc tgatgcgtcc
     4441 cagatggatg tgaaaccaac gaaaacctat aaagacggcg agttgttgcc tggaggcatt
     4501 tgggcgatac agctcaagga tcagaagtca ccaggggagt cggccctcta tattcagcag
     4561 gggaagggcg tgttgattgt cggggatgcc ttgattggaa agccagcggg ggcacttagt
     4621 ctgttgccct ctgagcgata tggggatgtt gcgaaggcca aggaagggct acgccggttg
     4681 ttgaaatata acttcgactc gattcttgtg ggggatgggg ctagcattgt attcggagcc
     4741 aaacaggcag tggagcaggt gctccaggcg taaaggatca tgccgcttcg aaacggactg
     4801 tccgaagaac tgaatcgcca gggcaacgag cactttgctc gagggcatta cacggatgcc
     4861 tatgcttgct acgcaaaagc cttggaatgt gatcgattga cgggtgacca ccgggcgctg
     4921 agtgccactt tgggaaacct cggaaatatt tgcgcggtca gcggacgccg cgaatcggcg
     4981 cagagctact accaggaagt cctggagtta caaaaggtgt taggcgacga taaaggtatt
     5041 gggacgacat tggccaatct ggggaatctg cgcgcagatg cgggggagtg ggatcgcgcc
     5101 agagcctatt acctcgaagc cctcgacatt atgacacgag tacatgatga actcggcaag
     5161 gctgtgctgt tttccgacct cggcttggta gcccgagaaa ccggacagtg cgatgacgca
     5221 ctgcgctact atgaacagtc gctggtcctg atgcgtcggc tgaataatca aggcggcgtg
     5281 gctgatgcct ggcggatgat ggggcggact ttcgcaatcc aaaagcgata cgacgatgcg
     5341 attgcctgtt gtcaaacaag tcaatccatt gctgaacgca gtcgagacga actgcgggcc
     5401 ggcggggcgc ggtacgtctt ggcccagtgt tatgaagatc tcggacagtt acaagctgct
     5461 gtagatttac tggagcaagt agtgcgaatg gatcgtaaat atgctctccc gaaattgacc
     5521 gaaaacacgg ctcggcttgc aagtctgcgt gctcgcctgg aagcggaaga tcccagtccc
     5581 caaccccggc ggtcgcgcgc atgagtacgg tggcacttcc agcctgggag gcggctcgtg
     5641 caaagctacg caccaatatt ccccaattct acgaattgga gccaggcggc cccctcgttc
     5701 ttgatttggg ggatgatgga tggatcttag aactgaggtc tgacggtcag ttcctttgtc
     5761 agcatggaat ctccatggat gatgtaaaga gtttgatgtg tgacgggacg acggaagatt
     5821 tgggaaccga tgaagtcgcc aagcaggcta agtattttct gggtcctgct gtatccaaga
     5881 aacgccctgc tctcctcaag gcagggttcg ctgagcagac ggaaatgaac gacgaatatg
     5941 tcgccatcac gttcctcaag gcggtgaatt tcgaacgcta cgaggaggtt cttggctccg
     6001 tgcgttggtg tcagaatctt ttcaagacgt aatcttcacg caccctaagg gcgccaattc
     6061 cccctgtttg cctcttttcc ctgtccgatg ctctcccttc agcctatccg tgctttgctc
     6121 gccgcgcata atcatggtca agtagaaccg caccctcttc ccatcaacgg gagccggaag
     6181 aatgcttggt aagcgcgtgc aagctggatc ctttgccaaa atcttgccct gtcaggactt
     6241 ggcctcagtg cgggtctccg ccagcagagg aattgcgatg tggggtgctg gagggtaggt
     6301 gaattacgac gaggaagaag atcgatattc ggactgatgg ctttctgcag gccgttgact
     6361 gggctgataa gtgagcatgc accgtcctcc cataatcata attaggaaat tgactagact
     6421 gcttgctaag acaaggatga ggacaaccag gcgaggagat ccttggctgg cttcctgaac
     6481 gaccgtggcg aggtcgagtg cgagggcaat agtgctgtat atgaccgctc ccatcacggc
     6541 ccacggctga cggatgaaga tcagtccccc gagcagcagc gcgggtaaaa cgaatagaaa
     6601 cccgactaag atgccgtctt tgatcgtcga gagaggggag cctgattcaa ctccaaagag
     6661 aataccctca aggacaatga gaacactcaa caggacgacg atgggacgcg catggagcat
     6721 agcggtacta taacggtggc atatccctct cgcaagctat cagattccgc ccatatctta
     6781 gtgccgggtc atcatcaaag ataatgatcg atcctcgggg tacgctgggc aaggtcaatg
     6841 acggctacct tccccatcct tcgaggggac caccaggtcc gttgagtcat ttaccctaga
     6901 tccgtattca cgtgaaaatc cgcctccatt agggaggcac acgtggccca ttacacatct
     6961 cacgtacgag ttcaacatcg gggagccaga gcatggtggc ccgcaacagt cacctcgcag
     7021 cgctctcttg ccatcgggcg atcgttaccc ctgctgctgt gcttgatggt agcggtgagt
     7081 tgcctgccgg gccttgcttt ggcggaagtg tatgactttc cggctcgggg ccataggggc
     7141 ggggccatcg ctgacccagt ggtacaaagg ccagccgacc attcgtcggt actagcggca
     7201 ggatttggtt ttcaggcagc aggatcgtcc ctcataacgg tccgaaccta tgatgttcag
     7261 acaggcgcca ttctttcaga ggattcattt gacgtgaacg tgaaggaaga gggcgccgct
     7321 gaaagcgatg ggagcaaggg gcgtattttc gccggaggaa tcggtgccga cagtcaggga
     7381 aagtctgggt ttatgctgag agtatatgat gcggagacag gaagatttct ctgggaaggg
     7441 caattgaact tgctcaaagt gaacgaagag ggaccgagta aagtgaaggc ggtgattgct
     7501 cccaaacctg ctgtcgtaat acctgcctca gatcgccctc ctcaagtctt ccaaacgttt
     7561 ttctccgtgc gagcagtcaa tcctcttacc gggaggcttg tttggcagga tcaatttact
     7621 ccaggcacta gaaaacgacc gggcagtgca ggggtgtcat gtaagaggtg ctcggtgagc
     7681 cctgtcaatg agcctatcgg acacattttt gacttggttg tgcgaaccta cgatcgcaca
     7741 tcaggcctgc tgttgtggga ggattcgttc gaggaaatcg atcgaattga tgagtctgat
     7801 tccgcgccgg agacaagtgc ctatccacag gcaataccat tttggcatcc gggggttgcg
     7861 gacaaaaccc ccatgtacaa agtgaccctc cgctgacagt tacgatttgg gcgtggataa
     7921 ggtggcgccc atgctctcca cccactgttg ctggaaatgg tcgtaggaga gcagcaggcg
     7981 gtcctgcatg gcagcggcga ttgtctggcg cgccttcaag tgaagaagta actcatgaac
     8041 cttgtgcatg ccgaatcgtt caataaggta gtgggtagcg gcattggctt ccatgtatgc
     8101 ggcgcccacc ttttcggccg ataagttttc ccagccctcc tcaagagccg ccagggggat
     8161 caagatatgt tcccccggag gcatgtttgc gacctcctgc caggaatcat tggccagctg
     8221 catagccaac ccttcattga gccaggtggg tatggctcca ccggttgctc ccagttcctc
     8281 atgaatcagc gcgtggacaa attcgtgacg gagaacgcga gtcagccagg cgcgatctgt
     8341 cgttgcgcct tgactgggga tctggatgcg ccccagaaca gggtcgaata agccatctgc
     8401 ccacacgggg ctgccggtgg atccctgaaa ctgagtcttg gtgtgcagca ccaccataat
     8461 aggtttcgac ggaaagaaat tgaacttctg gccgatgtct cgatacgccg cctcgagaat
     8521 atccagcacg gttgtccagg tctccggatc ctcactgccg tcatacttga ccgtaaaatg
     8581 gacgctgtta cgcgttgaaa acttctcttc taccttttcc gcgcgatgga ccttagccgt
     8641 cacgactttc acataagact gcagccctgg atcctttttt atgcgatcca ttgcctgtgc
     8701 gagatgtttg gccgcttccg acaagcgatc tttttcctgg aggaggtccg ccagcgccac
     8761 atgcgggaac ggctcatccg gagatagggt cagcaccttt ttcaagaatt cttccgtcag
     8821 cgctggatcc cgcagacccc agtaggcatg ggcaaggttg agatgggcca ccgggttttg
     8881 ggggtccagc atcaccgcct tcttaaaggc tttgacggag gtttcagttc cgccggcgtt
     8941 ttcttggaga ataccgaggt tgttccacaa gatggccaca tggcgttttg tggccgactc
     9001 agaaagaatc gacgagggga gttcttcgag tttcgtttgt gccaatgcaa tattgtgttt
     9061 ctcaagctcg tcgtgaatgg tgccgagcag ttcggagtgc cgagggacgg gaatgctgac
     9121 gggatcaatg gttctggatc gtttcatttc cggtagcacc gcttgttgcg gaggcgccac
     9181 gggacctttg gatccggcga cctcttctgt aatgacttga ggtggcggtg ctggcatctc
     9241 gtggaaatag tgcggcttga gccaggactg atagaaaatg aagatagcaa tgagaatggc
     9301 caacggcccg agaatatgct tgatattgcg tcgatacata ggtgtccttc cgtgagtcct
     9361 acacgaagtt actctagcat cgaggtagtg gacgaccaag gaaagtcgca aaagcgggcg
     9421 aatggtggtc acgggagggt gtgttcgtca tcgccggtcc ttcattgagc aggcaggcag
     9481 cctatggtac gatcccgcct aggatgccac gtcctgattc ttctgatccc catgagtctt
     9541 tccggtcatt cctgcagcga gtggcaagac ccattgaatt tgcttgccgt gatgcctatg
     9601 cccacctgcc gacagtaaaa aatcttgacc gttttgtgtc ggaacaggtc attggcgcgc
     9661 tcggtgagcg acttcacccg cgcgcgcttg aggttgagct gctctcctta cgcaatctgt
     9721 tcgtcgattt tcatgccggc ctcacggctg ccgaacagca gcatcgattg acgcaggccc
     9781 tccagctcct tcgccggctc caagaggagt cgccgatggt gcatccgcct gccgatcttc
     9841 tggagggagg ggagctggtt cacactcccg cgaatattca ttccagacag ctgtctgtag
     9901 agtcatcgat tcagttcgcg aagggtgttg gtccgaagcg caccctgctc ttgaaacgtc
     9961 tcggcatcca gacgatcgaa gaagcactct ggactttgcc atggcggtat gatgatcgat
    10021 cgaccatcac gccgattgct aagctgatgt tggcggcgac ggtctgtatc tgtggggtga
    10081 tcactcgaac ggaagcttcg cgcgcacgct ctcgccggtt gtcgattctg gacgtcgttg
    10141 ttcaggacgt aactggaacg gtgcacgcgg tgttctttaa tcaaccctat ctggaagacg
    10201 tgttgaagga gggggcacgc gtcatgatgg ccgggcgtgt agttgccggt cggcgaggat
    10261 ggacggatct tcggctggag ccgacgcaat ttgagatatt gagcggtcag gaagatgaat
    10321 tgctgcacgt cggacgcatc gtcccgattt accatgagac gaagggctgg acctctcgcc
    10381 agatgcgcgt cttaatccag ggggtactgg ccgggcacgg tgcggagctt gtggacgttt
    10441 tgccgttgtc cattcgcgcg cgccaccggt tgccgccaat tggcgaggct attcagcagg
    10501 tacatttccc gccgccgcaa accgacctgg ccgcgcttga ccgcggggta acggctgccc
    10561 atcgccgatt ggccttcgag gaattgtttc tgcttcagac ggccatggtg ttgaggcaac
    10621 agcaggtgaa ggacgaagag aaaccgtttc gattcagtcc tcatgtgccg gaactcaaaa
    10681 aactgtcgca gattttgccc ttctcactga cggctgcgca ggaacgggta tggcgagaga
    10741 ttcaagccga tatggcgacc tcgcgcccca tgaatcgtct ggtgcagggg gatgtggggt
    10801 gtggaaaaac ggtcgtggcg ttgcatgccc tggtgatggc ctgtggctcc ggttgtcaaa
    10861 cagcgctgat ggctcccacc gaaattctgg ctgaacagca ctatctgaat ctcaggcctc
    10921 tcctagaggc ggttggtttc aaggccgtgt tgctcacgag cggggggaag gccaaagatc
    10981 gccaggctgt cttcacgcag ttggcatcgg gggaagcgca ggtggccatc ggcacccatg
    11041 cgctgattca gaagaacgtc gcattcgaca agcttgggtt ggtggtagtg gacgagcagc
    11101 ataagtttgg tgtgctgcag cgaaagtcgc tgctcgacaa aggatatcga ccggatgtgc
    11161 tggtcatgac ggccacgccg attcctcgca ccttggcgat gactgtctat ggcgatctgg
    11221 atgtttcagt catcgatatg ttgccaccgg gccgcaagcc ggtgcggacc atgctctact
    11281 cggaaggtca gcgccacaaa acttggcagc tggtgagcga tgagctgaag gcgggacggc
    11341 aagcgtatat catctatccg ctggtagaag agtccgagaa gattgatctc aaagcggcga
    11401 tacagggagc ggaacagttg cagcgcgaca tctttccgca ggcgcagatc ggcttgttgc
    11461 acgggaagat gccgtcgtct gaaaaagagc gtacgatggc agccttcaag gccggcacaa
    11521 tacagatttt ggttgcgacg acggtcattg aagtgggggt ggatgtgtcc aatgccaccg
    11581 tcatggtcat cgaacatgcc gaacgattcg gactggccca actccatcaa ttgcgcggac
    11641 gagtaggacg aggggcgcat caatccttgt gtatcctcat ggcgtcctat ctcccgcgag
    11701 agtccagacc gaaggtgagt gctgaaggaa agcctgaggc caatgcctcc aatgcccagc
    11761 aacgcttgtc cgcgctggtg aaatcacacg acgggtttgt cattgcggaa gaagatcttc
    11821 gcatcagggg gcccggcgag ttcttgggat tgcggcaatg gggactgccg gagtttcggg
    11881 cagccaatct cattcgtgat acacacttgt tggagctggc gagacaagaa gcgttcgcga
    11941 tggtaaccca ggatccgggc ctggcgttgc cccagcatca ggcggtcaag gcggccatgt
    12001 ttcgtcgctg gaagggcaag ctgtccttgg gggacgtcag ttagttagtt ggcgcaatgg
    12061 tgctgccgaa ggagcggagg actgatcgat gggaatgctg caacgtttga gtcacgatct
    12121 tcgcgccgga tgggtgacat tacgacatgg cactgcaaag gctgccacga gagcattgga
    12181 agagggcgag cttctggggt atcgattgga attgcgaaag gtggagcagc agctggacga
    12241 tctgcatgtg gacattggtg agcggacgat cgagttgcat gaccgcggcg aggcaccgga
    12301 tcgtattgtg gctgattccg aagtcgcacg tctgatgaaa caggtgcagg cgctgcagga
    12361 tgaacggacc aagctcctat tggaaatgaa tgacatcacg gtagaagagg cctgaaagat
    12421 ttcatggctg gcgcatcaac aggttcgtcc agaatcttgg ctggcatcga tatcggaacc
    12481 ctgacctgcc gcctggagat tgctgaggtg acttcctcag gccaggtcaa aaaactgcga
    12541 tctgatcgac gaattctccg gctcggccag ggggtcgatc gagatcgcat gctgcggacc
    12601 gacgctatgg cgagagtggt gacgacgctg aaggaatggc gccagagtat tgatacatat
    12661 caggtggagg cgacgtcggt ggttgcgacg agtgccgtgc gcgatgccgg gaaccgacaa
    12721 gactttttag acctgatcgc acgtgaggcc ggcttgcaag tggaaatcat ctccggtgag
    12781 gaggaggcgc gacgaaccac cttggggatt cgttccggtc tgccggaagg tgtctctgac
    12841 atgctggcgg tggatatcgg cgggggaagt acggaatgta ttctggatcg acccggccaa
    12901 ccgctcattg tgcgatccat cgatattggt gtggtgcgcc tgagcgaacg cgtgctgcag
    12961 agcgatccgc ctaccgccga agaacttcag caggcgcatg attggatacg cactgagacc
    13021 ggagcggcca tcgcggatat gcccttgccg gtcggactga catttgtggg tacagcggga
    13081 acgatcaccg cgttggcggc tatggagcag caattgcccg tctatgaagc agcgaaaatt
    13141 cacaactatg ttctgacgct tgaggcggtg aagcgcctgg aggcacagct tcttggtcga
    13201 tcgaaacagc aacgggaggg gttgccgggg ctggagcgag gccgggagga cgtcattgtc
    13261 gccggagcga tcatcctcag tacgatgatg gagacgctgg gcatggcgaa ggttctggtc
    13321 agcgacctgg ggcttcgaga aggggtgctg ctccacctgg cgagccggtt gaacggaggt
    13381 tcctcgttgc gctgagtggc ggcctgacgg tgataggctc tcgttggtga agggatgacg
    13441 tgctgatcgt taccgttggg agtaccagcg cagcccgctt ttttgttcgg tgaaggtggc
    13501 ctgaggcatg ccgcctggtt tttccagaag taagaccttg aagtcgatca ggccgaacca
    13561 ggctggatcg gcaatatcgt ggtaatggca gccctgcagt ttcggaaggg tgtcaccgag
    13621 ggtcttcgtg agctcacttt cggtgtaggc cctcaccgag aagtgttggc caagtccctg
    13681 gcaaaaccgc tgaatcgtat aggctgcgcc ggtgcaaagg accttcgtat ccggtttgag
    13741 ctgtaaaaat tgcgggagcg acaaatagcg ctcctgaaag cccagccacc ggaccgcttg
    13801 ccgcaaatgg ctatactgca cttcgtattc ggtgtggtga ggaagtttca ccgcggcagg
    13861 ccagccttct tcgatcccga actccgtcaa aaatgcccga ccgcccggtt tgaggactcg
    13921 ccagacttcg gcgacgaatc gcatcggtcc cagattgaag atcacttctt ccggtagctc
    13981 tttgtcgagt ggcaaccgca gtcgcctgat ccaatccagt gcttcctgat gttgcgcggt
    14041 ttccccccgg ccactcgtga gttcctcccg gctgagcttg accggggtca tgtccgccat
    14101 gttttcgttg tcgatgatga ggtcgacgga gttatcctta aacggcagcc attccccgtt
    14161 ggcatgagtg ccggtgcctg tccagccacc ggccctggcg cgcttggttt gcagttggag
    14221 gaaggggcgc gtgacgtcca acgagatata ctggatgccc tgcttttcga acggcaacag
    14281 ctcttggccg agttcgcggg cgacatagcc gagcccgcct ccgatttcca cgatcacctt
    14341 gggtttcggt ttgaaccagc ccaggcggcg aagttgacgc atgagcaggc gtccataggt
    14401 ggtgcctccc agagcctcgc agggttctcg gaagaggtgc gaaacggtcg tttcaatgag
    14461 atcgaaatgg ccgtcctcca gatcgctttc tttcagctcg tgaacatgga aatcttcgag
    14521 atgttcttcg ccttcgaaat cctgttggcc ggaccagctt tctcgtattt gctggagcag
    14581 gaggtcccat ttagccttgt gccggtggcc ccccggcggt tcggtcccga aatagcagag
    14641 tgaatagttg gggatcgacc agtgtgccag atgccaggat cctggctgac cgtcaggccc
    14701 gcagatcttg ttgccgtatt tctccacaag tgcaccgacc gtaatgtggc cgttcatgga
    14761 ggtgagcatt tcaatgccga ctgcgttcag gcggacgagc gggaaatcat catccagggg
    14821 cgccaaccac cattcgtcaa aggcatgctg atacgcaacc aggtcgggaa tgcgccaggc
    14881 gagcaggctc agaatgtcgc cgctcagatt gagcggttct tgaatggctg cggcagcttt
    14941 catcgcgtca gacgattcag attacaagaa gccgcagcct acacgaggct cacgctgacc
    15001 tgcaagaacc caggagggtc gtctctaggt ttgtgagggc aggaggggag agtcggtcac
    15061 gatgatgatg ccgcgcataa tgggatgaat gcggcattga tacgggtatc ggcctggagg
    15121 caggctggga atggtataag tgccacccgg ttccaccgcg ccggagtcga aggcgcaacg
    15181 tccgctttcg tcaaaacagc cgtcgtgcgt cacggtgtga tgggtcggcg ttgggttgtc
    15241 ccaccggatg gcgccacctg cggtgacggt ggcggcagcg ggcacgtaat agggggaacc
    15301 gctatccatc aagacatgga ctgccggagg ggtcgcggag gagggccaag ccatcaggaa
    15361 tgcgaacgtg agacttagag agagtgtgcg ccagctcatg aggtacatga ctccatgtga
    15421 atatcgatct cttcattata catagagaca ggcgcgatgc ggaaggattc accagactgc
    15481 tcctaggctg ggcctctttg aaacagcgca aaccggatga ccgaatcgaa ggcaccaaga
    15541 gtgagtgtag gctgctggaa ggtttggctt ggcgttgcac gatgacacaa tttcgatgtg
    15601 aatcggagag gttgcaagaa cctattggtt catgctagat ggaggccgct ttaccaccga
    15661 cgtgccatgt tgaagacctg agaggaggac gtggaatggc gaagaagacc atgaaaaaga
    15721 aaagctctgc ggtgaaagtc gcgcggccga aaaagggcgc ggctttcaag gcgagcaagc
    15781 cgaagaaagt cgtgaaagcg aagaaggccg cacccaagaa gccggtgaag gccaagaagg
    15841 cggcgcaacc gaaggtgacg gcgaagaagc cggtgaagaa gacnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnccagctt gacctcgaag atgacgaaga ggacatggct
    16021 gaagatctgg atgacgtatc agaagaagaa atggaagaag aaatcgaaga ggagctcggc
    16081 ctcgacaatg aggacgacga cgtcgattat ctcgaaaagt ctgaagatct gctcgacgat
    16141 gcagatgagt atcgaaacaa ttagtttcag ttgatgccgc tgtaccgggt gatctcggtc
    16201 tgtgcctctg cgtgcctcat tgattctcgt cagtgaaggt cccactgaac atgtctgatg
    16261 tcatggtgtg ttgcgccgcg tctcgaatga agtcgcgtcg cgccttccga tcgtgtcgca
    16321 tcgttcgggt gcggctcgcg gcgacgtctg ccaattccaa tcgctcagcc ctctcatgag
    16381 ccactttctt tttatcgccg gggccttacg cgaacggact tccccggaaa gtgccgagtt
    16441 gcagctgggc gccgggatct ggggcctgtg tacctccttg atccgcgaca accttcaaaa
    16501 gttcctgacg cccgaatcac atggcctggt gtatgtgttg aaagaaggca tccgcgcgga
    16561 attttcgatt gtctcgccgg tccagccgtt tcaggcgttg gatgaactgc tgaaagatga
    16621 gttgcggacg gaagcccgct atggattcgt acgggtcaca ccggttcgac gctgggaggg
    16681 gaacgcgccc gattgccagc agctgcttca gcgagtcctg ggcatcgaag aacaatcaga
    16741 attgtcccgt cggctcggcc tggggatgca ccgtctttca caggaggaat accgagcgat
    16801 tgtcgacggg ttgggggcgg aacactaatc agatcgcgct tccttccgga agcggctagt
    16861 cgagggtgcc acctgtcatg agatggtagg cctcatccac cgtggcgact tctttgattt
    16921 ctacgttgag atcccaggca aggcgggtga gattccagcg ggggtcgtct cgttgacctt
    16981 gcgggatgag gatggtgcgg tatccctcgc gggctgctgc ccggatcttg tcggggatgc
    17041 ttcccaccgg gccgatcgtg ccgtccttgg aaatcgtgcc cgtcatggca atgccgcggc
    17101 ggatctgctc gcccttgagg agtgcggcaa atcccaccgt catgacgcct ccggcactcg
    17161 gcccgtcggt accggttgcg gcgtacgaaa tgccgatgac actgatggtc ccggtatggt
    17221 tcaccgtcgg tgtatgttcg agagcatatt gtaaggcgag gaccatagac ccaaggttcg
    17281 tctgtccggg tttgacgttc ccgccttgcc attgcagcat gatcggagaa ggggtcggct
    17341 gatgatccca gcgcagcaag agcacttcga acacgccaag ctggttggac tgaatagccg
    17401 ccaacatggg aacttcgacg actgactcag cggcagccgg ccgaaggtgg gtgaacacga
    17461 ggcagatata gaggggcaga aggcacagga ggcgtgagcc tgttcgacag agagacggga
    17521 agatggtggc tgacatatga gaacgagtct agcaaagcgc ggtatggtgt aaagtgttca
    17581 cgcaattctt cccctgcgag cgtagccaga gagagtgtct atccatggtg aattggccca
    17641 gacaactgca tcgaatttcc gcctggatac tcgggattct ctggggaacc gtcatgcttc
    17701 ccggtctctt gttcgccgaa tgtacgagtg agaccggtgg aaagactgcc agcgcagtct
    17761 tcactctgca cttgaccggc acctgtacgg aagcggagcg ggaggctcgt gccgttccgg
    17821 ccaaagatct catgcgagcc ttggcggccg ggaaaggcat tgatctggcg ggagtggtca
    17881 ttcagggcga tctggtgctt gacgagttac cggcgcagaa ggttgacgcg gtgcagggcc
    17941 tgtccctcga agatcgccgg gtgctcgaag ggctcaacga tgaagaggtg catgtcattc
    18001 gcgggccatt cgtgatcaag cactcccggg tgaaggggca gatcgtgaat cgtctcaagc
    18061 gcgggttttt gctgattacc ggcccggtgg tgttggtgca cagcggtttc gacggcctcg
    18121 tcgatttgtc acggacagtc tttctcggtc tggttgatgg gtcgaatgcc aagttcgaga
    18181 aggaaagtta ttttgttcag gaccgcttca cgcagggcgc gatgttttcc gatacccgct
    18241 tcggatcgca tgcacggttt catcggtcta tgtttagcgg tccggccatc tttcgcggcg
    18301 cagattttcc tggcctgact gagtttctgg aagtggtatt cgagcaggac gcgaattttg
    18361 ctcgtacggc cttccatatg ggaaccggat tctctggtgc gcactgtcgt ggcaagtgcg
    18421 atttttcatc gacgctgttt gagcgagagg cgttcttttt gtttgctcga tttgaccggg
    18481 cggtgacttt tgcctcggcg aaattcagtt cccaagccga tttttccgac gcgtccttca
    18541 aggaggctga cgacttagca aaggctacgt tcgttcgccc gccggtcatg atcagaactg
    18601 cgcgggtcgt ttcgactgtg cccgttgccc cggaagcggg tcctttttcc caagtggtga
    18661 cgatcgggct gtttgtggcg gcgttgggaa ttttgatcta tatcctcaaa gcgaagtagt
    18721 tgcctgaacc ccaacatata gttttagcct tgccccatcc caccataaat tggtataaga
    18781 ttgaaaattt gtccaaggga gcgactggta cctgatggac caacctgaac tgccctacca
    18841 agtcaagatc gagaattttg aaggtccgct cgatctcctg ttgcatctca tcaagaaaaa
    18901 cgaaatcaac atctacgata ttccgattgc gctgatcgct cagcagtatc tggactatct
    18961 gtcggtgatg aaggaattga acctcgcggt ggcgggggag tttttggtga tggcggccac
    19021 gttgctgcac atcaagtccc gtatgttatt gccaacagac gaagtcgctg tcgatgagga
    19081 agacgggccc gatccccgag aggaactggt tcggcgactg cttgaatata agcagttcaa
    19141 ggaggcagct tcgcagctgg attataagga acgcctctgg cgggatacgt ttggccgtga
    19201 gcccggtccg ccgatcgaaa tcgcaaaaga tgaaagcctg ctcgacgaga tttcgctgtt
    19261 tgatctggtc gatgcattgc aaggcgttct gtcccgacat cccggaaaac gcctggttga
    19321 aatcatcccg gacaacctga cggtccgtac acgcatgagc gcgattatcg aggcgcttga
    19381 ggtgcacgaa tctatgccgt tcacggcgct gtttgaggag tcgagtcatc gcctggtcgt
    19441 tattgtgaca ttcctggctt tacttgagct gatccgcatc agggtcgtgc gggtttttca
    19501 aagcgaaacc ttcggggcga ttcttgtctc ccgtgccttt acggcggtgg cagaagggga
    19561 tctagtggag gagtctgaat ggaagaacct atgagcctca gcatggagga cgccgtcgaa
    19621 cagcctgccg aggtggctga aatggaggcg ccagtgtccg aagagccgac tgctgaacct
    19681 gaggtagagg cccacgccgg cgaaggtgac gtggcggaga tggctgcgcc gacaccagcg
    19741 tctgatcccg ctctttccga cgtgcgagcc ttgaagggaa tccttgaggc cttgctcttt
    19801 gtgacgcccg acccaattcc tgtcacgcga ttcttggcct tgcttggggc cgtaacaaaa
    19861 caagaggttg atcaggcact ggccagtctg agtcatgact acgaacagga gggccgaggc
    19921 ctccagttag cggaggtggc cggaggctac cggatcgtga cgaaggctga atttgctccc
    19981 tggctcaagc gtctcgagaa ggtcaaggcg ccctcgaagc tctcgcggtc ggcgctcgaa
    20041 tccttggcga ttattgccta caaacagccg atcgtccgcg gagaggtcga gcaaattcga
    20101 ggagttgaaa cgtcgggtgt gattcggact cttttggaac gaaaacttgt gcgaatcgtc
    20161 gggagaaaag aggagcccgg acggccaatc atgtatggaa caaccaaggt gttccttgag
    20221 cattttggac ttcgcgactt gtctcagttg ccgcccctca gggagttcaa ggaattgggt
    20281 gagtccgagc aggcgatgtt accgatcgac gaatccgagg ctgtggggcc ggaagcgctg
    20341 tctcctgcgg ccgatactgt cttcgaggac aatgttgaag catccaacgg gtcggtagag
    20401 cttatagagt tgaatggctc tagcgaggct ctgccggagc cgtcggttga actatcttcc
    20461 gatggtgaac tgatggcggc ggaagtggca gaagagtcgt gattgccgcc tgctgataca
    20521 gggggctgat aagtcagggc aaagcccctc tttcgaagtt gttcccaatt tcatctccac
    20581 gttctccaac acaaaaggcc ttcgggatgc tcggcgagga gcacagcccg gaggcctttt
    20641 gtgtacgtgc accagcgtgc tgatttagcg gcagccgatt ggaatgaagc cggcgccgaa
    20701 aatccgcgac aatgtgacat agcgtgcatt gtcgtaaaac gagtagctcg gagcatacca
    20761 gttctgggcc gtagggtttc cgccataaga gggatccgtg ccaaagatgt gcccaccacg
    20821 gctgttctga ttgagatgga tccactccat atacatggag cagacttctc c
//
LOCUS       DAOQEW010000049        20853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_631_length_20853_cov_8.279306, whole genome shotgun sequence.
ACCESSION   DAOQEW010000049 DAOQEW010000000
VERSION     DAOQEW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20853
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_20853_cov_8.279306"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2066)
                     /locus_tag="PLS35_07455"
     CDS             complement(<1..2066)
                     /locus_tag="PLS35_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18532.1"
                     /translation="MHHLRSFQLRDMTACGAALRQSGAGAQSFDEVADRLVRHLYTSL
                     TMPQTGEPACALIRLFKTTSYEHLAPDLKVLADQRLGGTAGAPSMTCLTLSASAGMVP
                     GWNDHTLSSRFRVIPLRTSEELDKLPMFSQLFRQLGVGLPDLARPNTSLLVDPGEHAF
                     NVFHVPEAAGSPYVPTQEGFVEKYGVRSVLGFGAPLPDGQLFSIILFSQDVIPESTAQ
                     LFKPLALCAQTALAPYAERSATNSVKSVEPAKNQNLDESSTIDHLKSRIAELERLLAV
                     QEQTVTTQAERMDLVVRGSEAGTWDWHIGTGQVTFNERWATMLGYQLHELEPNVGTWE
                     QLVHPDDLPAVKERVSAHLRGETPLYSTEHRLKTKSGTWCWVFDSGRVVERDASGNPV
                     RAAGIHLDISARKELEAAQALAQRDLQVKQQALDEAQTLAHLGFWEWDIVTGDEQWSN
                     EQCRIFGFEPGTVKPTYATFLSALHQDDKSRVQQAIDAAITYDFPYNIDCRIHRPSGE
                     VRHINCRGTVRRKPDGSPLAMTGTVQDITAHKHAELAWRESEARMRSIFESAIEGIVV
                     IDEKGRIESVNAALLQLLGYEAHELIGQNISLIMPEPYRANHSHYLANYLVTGKRVII
                     GSGREVTAVRKDGTLIDIHVSVSEMQIGPSKKYTGMIRDISERKRMEETIRESEARFR
                     QLAESI"
     gene            complement(2167..2823)
                     /locus_tag="PLS35_07460"
     CDS             complement(2167..2823)
                     /locus_tag="PLS35_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18533.1"
                     /translation="MNETTYTPTRVFIINPQELVRVGMRTLLNSVPDFTVVGEASTQT
                     DALPLVLEQSPDIVIVYLRVQDGTGIETAREILTHLPGARLLFLADTLNDTILLSAVS
                     TGAHGYVLQDAGSETLMHALRSIMKGQAYLDPGVTRHTFAYLRKVADREPERGRHLLS
                     PQERRLLPLIAQGKTNKEIAAELGLSDKTVKNYLANVYSKLHLTRRSQAAAFYLKTIP
                     "
     gene            complement(3066..4391)
                     /locus_tag="PLS35_07465"
     CDS             complement(3066..4391)
                     /locus_tag="PLS35_07465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNI18534.1"
                     /translation="MTPQTSRRVVITGMGVISPLGSTVDLFWHLLSQGESAVRPITSF
                     DTSPFQACLGAEVRDFDPEDFLHRKQARRMGRATQFAVASAMMAARDSGIDLEQEDRG
                     SIGISIGTSIGGMKEAFEFHDAAKVTAYERVNPFTMGMTFPNAISSEVAIVLGLHGPC
                     ETYSIGCSSTANAIGRAYEWIKSGQASLVVAGGTEAPLHPSVYAAMNAGRALAPDERG
                     TIRNLPRPFDKTRCGMVLGEGAGCFILEEYEHARARGARMYAELEGWGFTCDAHSMVK
                     AATTGQEQQRAARLALSTAHWFPEEVDYVNACGLGTMELDALETHTVKQVLGTHAYRV
                     PISSFKAALGHAFAASGAFQLIGTAKAMEHQFIPPTLNLTNPDPTCDLDYVSGEGRAA
                     HIDRALINSFGFGGKNIVLALSRIDARVAATSPVAASAGYGLHHLAGVS"
     gene            complement(4433..5704)
                     /locus_tag="PLS35_07470"
     CDS             complement(4433..5704)
                     /locus_tag="PLS35_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18535.1"
                     /translation="MKQLAAHPAAMDATFTLLDLLQQRKTRRFGRGMTLPGGPLQYTS
                     HHDPVPLSREEERYLIYAAIGRSGRNLGDMQFVGRPGVSVGQGNALMNFNSRTVPSPC
                     SAQTTQLFYTNDDGVFFVADAAGPDHPWDLNVIQLQSSRLDIPREAPFMLPFNQWYTN
                     RPGTTLFMPVTNIASLYLNLLLMMFSEETGYFIVDTDNGNAACGLEAFRKSAGGHLHD
                     DIKARRMFSLRELDAAICETAIQEQGIICEHLSLMQQALGLGGGIQSVGSGRHLLGME
                     PHIYPGLGFHFVVPPGKPLRANPVGIPGVWEGPTPPFVPSMKDAVTNLVESKFGADGT
                     FRKPQEQPYVQRNTAQQVPQHSERAIEATIAFTEYVLATYGRFPAHADACKSIVACQT
                     HHLDEDFYATFYPDSALPDAHREHMHTWHSH"
     gene            complement(5935..6732)
                     /locus_tag="PLS35_07475"
     CDS             complement(5935..6732)
                     /locus_tag="PLS35_07475"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI18536.1"
                     /translation="MSQPAFSAKEFQRLGEIMYDARSVNRNDGVWQLVSAVQRVVPYE
                     FSGCGAVDLLHGIDPSLGHSTYPREFCQLYMGQGLAADPAVNRLLTSGQTVTSSADEP
                     VGNEPRDVTSLKLDFGIKTCLSAGVRGDHGACSYFAFSNFDAKQADKLRLLLDILTPH
                     FHLSYMRCKSAWTPDRPTQPALLLSKREEEILRWVAAGKTNWEISVILKVSLNTVKFH
                     LKNIFQKIGVENRWSAIAYWQNGEQHRIIPPSAPSDDRSPSDPNRPT"
     gene            complement(6981..7958)
                     /locus_tag="PLS35_07480"
     CDS             complement(6981..7958)
                     /locus_tag="PLS35_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18537.1"
                     /translation="MRRFAGALLLCCAAAVPNLGFAESSSEHLDWGRWSFDYEVKDNT
                     GLAIRNVAYDGELVLGKASMPVLRVKYVKERVWWNPFTWFGSRADSGRCGPFQDRLRW
                     VDLVPIVNCGDNKVCVESATSHGVKWLELGIYARIGEYHIYQSWHFSEDGELRPVIQS
                     RGLSCNTDHIHHPYWRFDFDVNGNGMDQVFVRDNGGPDKGWGPGWTKYTNERNDVKHA
                     STQRTWFVRDQPTGHGVWLLPGDGYAPLKDDGERDSFADHDVAIRRAKPDEDVPWVFG
                     ARGQLGYDEDNQGVQEQDVVVWYVAHLPHMAALGPTKWLTLGPTLKIQR"
     gene            complement(7977..>8459)
                     /locus_tag="PLS35_07485"
     CDS             complement(7977..>8459)
                     /locus_tag="PLS35_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18538.1"
                     /translation="AEADSRVRSLLGERWGFVDADPMPPTSKITFGCCRQQARFTKLT
                     YFSYSHNVAVQVQLKDGTVLQASRIDGYLPPEGPQDVQRGIELARADARLAGKVNGLQ
                     GHGLLMQPDRGFFKNDPGYGHRVIWITFSQGVGGDPKYWAQVDLTDDRVLDAGDEPPR
                     "
     assembly_gap    8460..8559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8600..9121)
                     /locus_tag="PLS35_07490"
     CDS             complement(8600..9121)
                     /locus_tag="PLS35_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18539.1"
                     /translation="MNRSIDGVITFLRDNDNVIKQLMETAESATNDIEQLATSVDHAR
                     EEAQVLVDQLGAVEFSVGRDDDTKVRQTALLEAVAALRKKFELYKNDPLRRGIYAAEI
                     SNLYLQLANTFNNETVAKIVPFEADEIETYKDLMSKAVLDAASRKKRAAVIKAGVQIS
                     RLALGVAGKLALV"
     gene            complement(9133..9843)
                     /locus_tag="PLS35_07495"
     CDS             complement(9133..9843)
                     /locus_tag="PLS35_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18540.1"
                     /translation="MVRRGMTLLSLAMLLLMATGCHSIHSRSVRDLIQMEGTKIDAAQ
                     TNIDLFQKETEERITFLEQARSSLHESFKSLQIQEAKHRFALASFRNLTNKRGEAAYA
                     AAYLTSLIYMTDVQGLEKAVWDQFEEDFCALRDTANALNDSWKHTATLHAEVQRFAKQ
                     SALASVDPEFVAAILDQAPGRSEQIMSVLNHSRTVNDALEEVVGARILRVGGLDRAQR
                     FTADLVELLDRLKKDDGQ"
     gene            complement(9879..10511)
                     /locus_tag="PLS35_07500"
     CDS             complement(9879..10511)
                     /locus_tag="PLS35_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18541.1"
                     /translation="MRMLRIAGTLLFASLAACSTPPEIKQALAAKDQAYAENEQLMQQ
                     YRELVSNVTTRHLKWYRYVQTRLKLDLALQWATTNPRVADVPDAALAEDDAAALGPDV
                     LTLVNAMRLKSLPERKGPNGQPLFAAGSGDMSTLIQQLPDLIARVEQQVAAGAKIPQT
                     VDLTAFDRYRNNVEALRRINGLIKQYLDIDVTASRSDVQSLADALRTVHR"
     gene            complement(10759..12573)
                     /gene="uvrC"
                     /locus_tag="PLS35_07505"
     CDS             complement(10759..12573)
                     /gene="uvrC"
                     /locus_tag="PLS35_07505"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNI18542.1"
                     /translation="MAGSCETLQSKLDHLPEQPGCYLFRNAKREILYVGKAAVLADRV
                     RSYFQKGSDQTPKTSLLVSEVADLETIVTRSELEALILESNLIKRHRPRFNIVLRDDK
                     QYPYLRLPIKESFPRLSIVRRVQKDGALYYGPYTPAGALRETLKVIRKAFPLATCDID
                     IDGRADRACIEFEIKRCMAPCTGNQSREDYHVIVKQVRQFLEGRDRELLDSLRQAMES
                     AAEREEFEEAARLRDRLFSVERTLEKQRITQVTTTDQDVIGIARQGTAADLQLLFVRG
                     GLLIGRKDFFWPQSADSPDEELVRSAIEQFYNKEGQPPKELLVPTDLVDAPLIEQWLS
                     DKRGDTVRLLTPERGAKHQLVLLAEENAAAAIADHLRNEALDRQATAELKRLLRLDTV
                     PRRIEGFDISNIMGNQSVASLVVWEDGQAKKSDYRKFRIQTVEGANDFASMQEAVMRR
                     YGATEDLARPDLILIDGGLGQLSAALEGLKQVGQEQIPIMGLAKARGEKDERIFLPGR
                     KNPIVLRPTSPATHLVQRIRDEAHRFAITYHRNLRGKALLSSELDQIPGIGKIRRSRL
                     LKQFGSLPQIASATDEQLKSAGLDATTVAALRKTLIPS"
     gene            complement(12695..13564)
                     /gene="dapF"
                     /locus_tag="PLS35_07510"
     CDS             complement(12695..13564)
                     /gene="dapF"
                     /locus_tag="PLS35_07510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNI18543.1"
                     /translation="MKNGFFRGHGLGNDYVVMDPKELSFNLTPGKIRGICDRHWGLGS
                     DGILALVPSKKADFGLRIYNPDGSEAEKSGNGLRIFARYLHATGKTKKRAFTVETKGG
                     LVSITLHVDRHGDAAAATVEMGRATFQPSALPCTLSVEELIQAPIEAAGRSLQFTGVS
                     VGNPHCVVFKEAGQSWTREELLTLGPALENHPLFPKRTNVQLAVPTGPKEIFILIWER
                     GAGETQASGSSSCAAASAAVKLGLVKGPVKVIMPGGTLNIEVAKDFSLTMKGPVAEVA
                     RGSLSPSFVRTLR"
     assembly_gap    13581..13680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13681..14621)
                     /locus_tag="PLS35_07515"
     CDS             complement(<13681..14621)
                     /locus_tag="PLS35_07515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI18544.1"
                     /translation="MQPIDSRAQALIRDMREEDRDAVIAILTNSDPWKRLGFAASDWL
                     RIFAPLPAGRDTFILEVEGAVLGIAIVRRKFLFGDYLELLGIAPSATGRGFGSSLLSH
                     IESLTFARAQNLFACVSDFNEAARAFYRKQGYKEIGPMPNFLIPGYAEILLRKTAGPA
                     RKWRHTIETADGDHVPQTVNGVPLMKVTKLLHTRMRVSDIDQTIRFYQNVLGLEVMDR
                     KVSPRGSHLAFLKVPNSDELIELCSFPASGPVTVQEDLVHLAFEVENLDETIRSLESK
                     GVRITDGPTPTSSGSRFLFIDAPDGYEVELIERPPGTA"
     gene            complement(14588..15265)
                     /locus_tag="PLS35_07520"
     CDS             complement(14588..15265)
                     /locus_tag="PLS35_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNI18545.1"
                     /translation="MVFITEARPSSAQVIKSGPASCPAVALTYDLCPVRTASGFDREL
                     IDYLIEHKVPTTFFMSGRWMIKHDAEVKQLLAVPFFEIGTHGDVHAHLPMHDVEEQQQ
                     EIMGPVRLLKTKYGRPATLFRPPYGEYNDETVDAVKALGLRFILWNIESGDPDPALSA
                     DAILTRVQKRLKPGSVIVLHANGKGKHTREVTEALAGQLLPDKGLAPMTISDLLRCNQ
                     STVAPKP"
     gene            15382..16557
                     /locus_tag="PLS35_07525"
     CDS             15382..16557
                     /locus_tag="PLS35_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNI18546.1"
                     /translation="MQGPDWKIGRMFGIPIHVHASWLFVFFFMTWSLATGYLPDTLPG
                     LSEPRYWAMGGVAALLLFASVLLHELGHSLVALRYRIPIGQITLFIFGGVAQMRKEPP
                     HPRAEFLIAIAGPLVSFILAGLSLVLVAVLELFPAESSLHGLMALGTLLGMVNTQLGL
                     FNLLPGFPLDGGRALRAGLWAWSKDYYRATSQAALVGLLFGVTFALFGALLLFGALSG
                     TASSALASSGGWIVLLGAFLFTAARGTRKQAAIRASLASVPVRELMVRNVVALSPDLS
                     LEEAVNQYFLPYGYGGFPVVHEDRLLGVVAVRDIQSVKNSLWAFRRVADIMQPASDDM
                     VVSPDLSAMQALEQMIAVGAERLVVVQDGQLLGLVTRASIGHFIEQHHPSGLGKSSS"
     gene            complement(16633..17484)
                     /locus_tag="PLS35_07530"
     CDS             complement(16633..17484)
                     /locus_tag="PLS35_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNI18547.1"
                     /translation="MTELTFLSNVFDTFRRRGCPSLAASLAFYSILSLFPMVFLLLYG
                     ISFIVSQDVIGYQFLLGFLKGFLPSMGERLAKDIRRVAEQEEVRWAVFLAFGWFSALV
                     FYELDYAMNTVFGTAAKRHPLISTLVAVALIWMLGFLTLISFVATQAIELLTTYAPRF
                     LGMNLVAIAAHDFLITYTLPFLLAFASVTCLYRFLPHQRPTWREATIGGAVFSLLWVS
                     AKVLFVTYLEDAAVYTQLYGSLLEVVLLLLWVYYSSALVLLGAVVTYECQCRRPALLD
                     ESATLPG"
     gene            complement(17620..19095)
                     /locus_tag="PLS35_07535"
     CDS             complement(17620..19095)
                     /locus_tag="PLS35_07535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNI18548.1"
                     /translation="MTFPLQDLLAILPELIVIGTACLILALDPILGADKKDVLAWLTL
                     GALAMCMGLTSSHLTGQTYAFSNLVVIDSYGAFWKLLLYIVTGLTVLLSLAYLKAERL
                     SIGEYYGFILLALVGMMVMVSGADLLTIYLGTELMSLSLYVMAGLKRTEARSLEASAK
                     YFVLGAFSSGVLLYGISLLFGLAGSTRLSVIAEAITTHGTTNPMLSLALVLLAVGFGF
                     KLAVVPFHMWTPDVYQGAPTSVTAFMAVASKAASFGAFLRVFVEGLGGASADWSVLFT
                     IICLATLALGNLVAIVQTSIKRMLAYSSIAHAGYALIGVVVAARHSGEGTSSGFASVL
                     LYIAVYSFMTLGAFALVGMLRKEGQESENIEDYAGLAKREPLAAFFMLVFLVSLAGIP
                     PTAGFIGKFYIFMAAVNGGMTWLAVVAVIFAAISAFYYLRIVMVMYMREPEGAATAHS
                     RLETSPALSFVLACALAGVVFLGLFPNGLWSLATHAAPLLK"
     gene            complement(19092..20651)
                     /locus_tag="PLS35_07540"
     CDS             complement(19092..20651)
                     /locus_tag="PLS35_07540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNI18549.1"
                     /translation="MTSFPWLSLIVFFPLIGAVICFLVKAESSRWVALGFTVGNFLIS
                     LPLWWLFDSSTANMQFVERASWISSPPVNYSLGMDGISFPLVLMTTFLMPFCVTVSWT
                     AIEKRVQLFMSMLLVMEAAMVGVFVALDFVLFYVFWEVMLIPMYLLIGVWGGPNRLYA
                     AIKFFLYTLAGSLLLLVAILALYFQGGHTFDILELSRGTYGASLQMWLFLAFFAAFAV
                     KVPMFPFHTWLPDAHVEAPTAGSVILASVLLKMGTYGFLRFTLPMLPDATVAFTKPMI
                     ALSIVAIIYGAYMALAQSDIKKLIAYSSVSHMGFVTLGIFVLNIQGIEGAVMQMVNHG
                     ITTGGLFLCVGIIYERTHSRQILDNTGLAGPMPRYAMFLMIFALSSLGLPGTNSFVGE
                     FLVLAGTFVWSQFATAFAALGVILAAAYILWLMQRVVFGTPAPAHRAHLLDLNARETA
                     TLVPLIVLVFVLGIFPNPLLTRMHASVTNLLNAQKAPAYATAPQQPALPQPAATMAAM
                     SSSSSEQAAAR"
BASE COUNT         4199 a         5809 c         6309 g         4336 t
ORIGIN      
        1 tcgatcgatt ccgccaattg ccggaagcgc gcttcgctct cacggatggt ttcttccatc
       61 cgcttgcgct cggagatatc ccgaatcatt cccgtatatt ttttcgaggg gccgatctgc
      121 atttcactga cggacacatg aatgtcgatg agagtgccgt cttttcgaac cgcggtcacc
      181 tcgcggccgc tgccgatgat cacccgtttc cccgtgacca ggtaattggc aagatagtgc
      241 gagtggttgg cgcgataggg ctcaggcatg atcagcgaaa tgttctggcc gatcaattca
      301 tgcgcctcat atcccaacag ctgtagcagg gccgcattga cgctttcgat gcggcccttt
      361 tcatcgatga cgacgatacc ctctatcgcg ctttcgaaga ttgagcgcat gcgggcttcg
      421 ctctcacgcc atgctaattc ggcgtgtttg tgggcagtaa tgtcttgcac cgttccggtc
      481 atggcgaggg gagaaccgtc gggttttcgg cggacggttc cacgacagtt gatgtggcgc
      541 acttcgccgc tcggccggtg aatccgacag tcgatgttat aggggaagtc gtaggtgatc
      601 gccgcatcaa ttgcctgttg gacgcggctc ttgtcgtcct ggtgcagggc ggagagaaac
      661 gtggcatagg tcggcttgac ggtccctggc tcaaagccga atatgcggca ttgctcgttc
      721 gaccattgtt cgtcccccgt gacaatgtcc cactcccaga agccgagatg agccaatgtt
      781 tgcgcctcat ccagcgcctg ttgtttgact tggagatctc gctgggcgag agcctgggct
      841 gcttccaatt ccttgcgcgc ggaaatatcg agatggatgc ccgcggctcg gacgggattg
      901 cctgaggcat cgcgctcaac cacacggccg ctatcaaaga cccaacacca ggttccggac
      961 ttggtcttca ggcgatgttc agtggaatag aggggcgttt cgccgcgaag atgcgccgag
     1021 accctctcct tcacagcggg taggtcgtcc ggatggacga gctgctccca ggtgccaacg
     1081 tttggttcga gttcatgcag ttggtagccg agcatggttg cccagcgttc gttgaacgtg
     1141 acctgtccgg tgccgatgtg ccagtcccag gtgcctgcct cagacccacg gacgacgaga
     1201 tccatccgtt cagcttgggt ggttaccgtt tgttcctgaa cggcaaggag tcgctccagt
     1261 tcagcgatgc gtgacttcaa atggtcgatg gtcgaggatt cgtcgaggtt ctggttcttg
     1321 gcaggctcga cggatttgac ggagttcgtg gccgacctct cagcataagg cgccagggcc
     1381 gtctgcgcgc aaagagccag aggtttgaac agctgggcgg tgctttcggg gatcacatcc
     1441 tggctgaaga gaatgatcga gaagagctgt ccatcgggca gcggcgcgcc gaatcccaat
     1501 accgatcgga ctccatattt ctcgacgaac ccctcttgcg tcggtacata ggggcttccc
     1561 gccgcttcag gaacgtggaa cacgttgaag gcatgttcgc caggatctac aagcagactt
     1621 gtgttcggtc tcgcgaggtc aggtaatccg acacccaatt ggcggaacag ctgactgaac
     1681 atcggaagct tatccagctc ctcggatgtg cggaggggaa taacccggaa gcggctcgac
     1741 aatgtgtggt cattccagcc tgggaccatg ccggcactgg ccgagagggt caggcaggtc
     1801 atggatggag cgccggctgt tccgccaagc cgttgatcgg ctagaacttt gaggtctggc
     1861 gccagatgtt cgtaggaggt ggttttaaac agtcggatca aggcgcaggc cggctctccg
     1921 gtctggggca tggtcaacga cgtgtaaaga tggcgaacga ggcggtcggc aacttcgtcg
     1981 aagctctgcg ctccggcacc cgattgccgg agtgccgctc cgcaggctgt catgtcacgg
     2041 agctggaagg atcgtaaatg gtgcatgctc aggtgatccc gcggaggcga gagagtccgt
     2101 cggtactctg tcggcgctcg cgcggaagaa cttgagcagc gcaccagtcg ctgttaggcc
     2161 gttcccttag ggaatggttt tcaagtaaaa ggcggcggct tgggacctcc tggtgagatg
     2221 gagtttggag tacacgttcg ccaggtagtt cttgacggtt ttgtcgctga ggcccaattc
     2281 cgcggcaatc tccttattgg ttttgccctg tgcgatgagg ggaaggaggc gccgttcttg
     2341 cggcgacagc aggtgccggc cccgttcagg ttcccgatcg gcgactttcc ggagataggc
     2401 gaaggtgtgc cgggtcaccc cgggatcaag atacgcctgg cctttcatga tactgcgcag
     2461 cgcatgcatc agcgtttcgg atcccgcatc ttgtaggaca tatccgtggg cgccggtgga
     2521 aacagccgac aggaggatgg tgtcgttgag ggtgtcggcc aggaagagga ggcgggctcc
     2581 cggtagatgc gtcaagattt cccgagccgt ctcaatcccc gttccgtcct gcacgcgcaa
     2641 atacacaatg acgatgtcag ggctctgttc gagcacgagg ggaagcgcat cagtttgagt
     2701 ggatgcctct ccgaccacgg taaagtccgg gacagagttc agcaatgtgc gcatgccgac
     2761 tcgaaccaat tcctgcggat tgatgatgaa gacgcgtgtt ggggtgtaag ttgtttcgtt
     2821 catattcgac tctcacctaa atgtggatgg atgcctggtt gttcctgtat atgcctgcgc
     2881 ccagaaaccg gatgtcctga tggttgtgaa gaggcgctcg gccttcggtg tccaccctgc
     2941 ttgctgtgac cgacagtgac actgacgtgg catcgtgcgg cccggcctga cacagaccgg
     3001 gccgcataga ccgctgctgg tgtgatgccc gtggtcatga gtctgcggtc cttcatgtcg
     3061 tgagcctatg acactcctgc taaatggtgc aggccatatc ctgccgatgc agccactggg
     3121 ctggtcgctg ccaccctggc atctatgcgg gacagcgcta gcacgatgtt tttgccaccg
     3181 aatccaaaac tattgatcag cgcacggtcg atatgtgcgg cccgcccttc accggacaca
     3241 taatccagat cgcaggtggg atccggattc gtcagattca atgtcggcgg aatgaattga
     3301 tgttccatcg ccttggccgt cccgatgagt tggaaggcgc cgctggcggc aaaggcatgg
     3361 cccagtgccg ctttaaatga actgatgggc actcgatacg cgtgggtgcc caagacctgc
     3421 ttcacggtgt gggtttccag cgcatccaat tccatggtgc cgagaccgca cgcgttgaca
     3481 tagtcgactt cctccgggaa ccagtgagcg gttgacagtg ccagcctggc cgctcgttgc
     3541 tgttcctgac ccgtggtggc cgctttcacc atcgaatggg catcgcacgt aaagccccag
     3601 ccttcgagtt cggcatacat cctggctccc cgcgcgcggg catgttcgta ctcttccaga
     3661 atgaaacagc cggcgccttc gccgagcacc atgccgcaac gcgtcttgtc gaacggtcgc
     3721 ggaaggtttc tgatcgtgcc tcgttcatcc ggcgccagcg cccgtccggc attcatggcc
     3781 gcatacacac tcgggtgcag gggggcttcc gttccaccgg caacaaccag cgaggcctgg
     3841 cctgatttaa tccattcata ggcacgcccg atcgcgtttg ccgtcgagga acaaccgatg
     3901 gaataggttt cgcaaggacc gtgcagcccg agaacgatcg cgacttccga cgaaatggca
     3961 ttgggaaagg tcatgcccat ggtgaaggga ttgacccgtt cataggcggt gactttagct
     4021 gcgtcgtgaa attcaaacgc ttctttcatg ccgccgatgg aggtgccgat gctgatgccg
     4081 atcgacccgc ggtcttcctg ctccagatcg atgcccgaat cgcgcgcggc catcatggcg
     4141 gaggccacgg caaattgggt cgcccggccc atgcgccgcg cctgtttgcg atgcaggaag
     4201 tcttcgggat cgaaatcccg cacttccgct ccaaggcaag cctgaaacgg tgaggtgtcg
     4261 aatgacgtaa tcggccgtac cgcgctttcg ccctggctca ataagtgcca aaacaaatca
     4321 actgtcgaac ctaatggaga aattactccc attccggtga tgacgactcg acgagaggtc
     4381 tgtggtgtca tgatgccctc cagtgctcgt aagagcgtga aaatggttgt cgtcaatggc
     4441 tatgccacgt gtgcatgtgt tcgcgatgcg cgtccggcaa cgcggagtca ggataaaagg
     4501 ttgcgtaaaa gtcttcgtcc agatgatgcg tctgacaggc cacgatcgac ttgcaggcgt
     4561 cggcatgcgc cgggaaccgg ccgtacgtcg cgaggacata ctcggtaaag gcgatggtcg
     4621 cctcgatggc gcgttcggaa tgctgcggga cctgctgtgc cgtgttgcgc tgtacgtagg
     4681 gttgttcctg tggtttcctg aaggtgccgt cggccccgaa cttgctctcg accaggttgg
     4741 tcacagcgtc cttcatcgat ggcacgaacg gcggcgtggg accttcccag actccaggaa
     4801 tgccgacggg attcgcgcgc aatggcttgc ccggggggac gacgaagtgg aatcccagtc
     4861 cggggtagat gtgaggctcc atgcccagga ggtgccgacc gcttcccacg ctttggattc
     4921 cgcctccgag cccaagggcc tgctgcataa gggagagatg ctcgcagatg atcccctgct
     4981 cctggatggc cgtttcacaa atcgccgcat cgagttcacg caggctgaac atgcggcggg
     5041 cttttatgtc gtcatgcaga tgcccgcccg cgcttttccg gaaggcctcg agcccgcatg
     5101 ccgcgttgcc gttgtccgta tcgacgatga aatagccggt ctcctcgctg aacatcatca
     5161 acaagagatt cagatacagg gaggcgatgt tggtcaccgg catgaagagg gtagtgccgg
     5221 gccgattcgt ataccattga ttgaacggaa gcatgaacgg ggcttcccgg ggaatgtcca
     5281 atcgggatga ttgcagttgt atgacgttca gatcccaggg gtgatccggt cctgcggcgt
     5341 cggccacgaa aaagaccccg tcatcgttgg tgtagaacag ctgcgtagtt tgcgcagagc
     5401 agggactggg gactgtgcga ctgttgaaat tcatcagcgc attgccttgt ccgacgctca
     5461 cccccgggcg tccaacaaat tgcatatcgc cgaggttgcg tcctgatcgg ccgatcgccg
     5521 cataaatgag atagcgttcc tcttcccggc tgagtgggac cgggtcatgg tgactcgtgt
     5581 attgcagcgg accgccaggc agggtcattc cgcggccgaa gcggcgcgtt tttcgctgtt
     5641 gtaggaggtc gagcagagtg aatgttgcgt ccattgctgc aggatgtgcg gcaagctgct
     5701 tcatagggcc tctccttccg gtttccctgg ggccattcgg ccctggccgg tcatgtcagt
     5761 ggcagtgata cgttgtgctg acacgtcacg ttgcgcgtga cgtgtgtatg gggcagactt
     5821 tacgagaggt atggctcggc gtcacctacc catcggggta ggcgaaggct ccgttcccgg
     5881 tcggtcaggt agtgacggac tgggcggcgc gcgggtcgac atgcatggtg cgagttaggt
     5941 cgggcggttg ggatcggacg gagaacgatc gtcactgggc gccgatggtg gaatgatgcg
     6001 atgctgttca ccattttgcc agtaggcaat ggcgctccac cggttttcga cgccgatctt
     6061 ctggaagata tttttgagat gaaatttgac cgtattcaaa ctgaccttga gaatgacgga
     6121 gatttcccaa ttcgttttgc ctgccgccac ccatcgtaaa atttcttctt cccgtttact
     6181 gagcagcagt gcaggttgcg ttggccgatc cggggtccac gctgatttgc aacgcatata
     6241 actcaggtgg aaatggggag tgagaatgtc gaggagcaga cgcagcttat ccgcctgttt
     6301 ggcgtcaaaa ttactgaagg caaagtagga gcaggctcca tggtccccgc gcacccctgc
     6361 cgaaagacag gttttgatgc cgaaatcgag cttgagcgac gtgacgtctc tgggctcatt
     6421 gcccaccggt tcatcggcgc tggaggtgac ggtctggccc gaggtcagca aacgattgac
     6481 agcaggatcc gccgcgaggc cctggcccat atacagttgg cagaattctc gagggtacgt
     6541 ggagtgtccg agagaaggat cgatcccgtg gagaagatcg accgcgccgc atcctgaaaa
     6601 ctcgtacggc acgacgcgct gcacggccga caccagctgc cacaccccgt cattccggtt
     6661 gacggagcgc gcgtcataca taatctcgcc gagtcgctga aattcctttg cggaaaacgc
     6721 cggctgactc atgccaattc cttccggcga acgcaccctg tcccgatagg gatgatcagc
     6781 aaccagaggc gttcagaaat tcggccgtgg ttcatgatga ggcagctgta tcatcgtgag
     6841 aacggctgat tgtactgcga ccgaagggac ggtatctagg gtaaacggtc cctactatag
     6901 agtgcctagg gtatgaaccg caacggggaa gaatacacgt attatttggt cggacgggga
     6961 gagaggcagg gaagaacggg ttatcgttga atttttaagg taggccccag tgtcagccac
     7021 ttggtcgggc caagggcagc catgtgcgga aggtgtgcca cataccagac caccacgtcc
     7081 tgttcctgga cgccttggtt gtcctcgtca taccccagtt ggccgcgtgc gccaaagacc
     7141 cagggcacgt cttcatcggg cttggctcgg cgaatcgcaa catcatggtc ggcaaacgag
     7201 tcccgctcgc catcgtcctt gagcggggcg tacccgtccc cgggaaggag ccacacgcca
     7261 tgccccgtcg gttgatcgcg cacaaaccac gttcgctgag tcgaggcgtg tttgacgtca
     7321 tttcgttcat tggtatattt ggtccatcca ggcccccagc ccttatctgg tcctccgtta
     7381 tcccgtacaa agacttgatc cattccattc ccgttcacgt cgaaatcgaa ccgccaatag
     7441 gggtggtgga tatgatcggt attgcaggat aaccctcggc tctggatgac cgggcggagt
     7501 tcgccatctt ccgaaaaatg ccaggattga taaatgtggt attccccgat gcgcgcataa
     7561 atgccaagct ccagccactt gacaccatgg gaggtggcgc tttcgacgca caccttgttg
     7621 tcgccgcagt tcacgatcgg caccagatcc acccagcgca accgatcttg aaatggcccg
     7681 caacgaccgc tgtccgcacg ggaaccgaac caggtgaacg gattccacca gacgcgttct
     7741 ttgacatatt tcacccgcag gaccggcatg ctggccttgc ccagaaccag ttccccatca
     7801 taggcgacgt tccggatggc cagtccggta ttgtctttga cctcgtaatc aaagctccag
     7861 cggccccagt cgagatgttc cgacgaggat tcggcaaacc ccaggttcgg cacagccgct
     7921 gcgcaacata gcaggagggc acccgcaaaa cgcctcattg gttctccttt cctgccttat
     7981 cgaggaggct cgtcgccggc atccaatacg cggtcatccg tgagatccac ctgggcccaa
     8041 tacttcggat caccgcccac tccttgcgaa aatgtaatcc agataacgcg atgaccatac
     8101 cctggatcgt ttttgaaaaa cccgcgatcc ggctgcatca ggaggccatg cccttgtagc
     8161 ccgttcacct tgccggccag cctggcatcg gcccgcgcca attcgatccc gcgttgaaca
     8221 tcctgcggcc cttcaggcgg caggtacccg tcgattcgtg acgcctggag taccgtcccg
     8281 tctttgagct gtacttggac cgcgacgttg tggctgtagc tgaagtatgt gagcttggtg
     8341 aaacgggcct gttggcggca acagccgaag gtaattttgc tggtcggcgg catcgggtcc
     8401 gcatcaacga atccccagcg ttcccccaga agagaccgca cacgagaatc ggcctctgcn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcggcgctgt tcagcatgat
     8581 gaatcgtgtc gccatcgcgt tataccagcg ccagttttcc tgccacgccc aatgccagcc
     8641 gggaaatctg cactcccgct ttgatgaccg ccgcgcgctt tttacgggac gcggcatcca
     8701 acaccgcttt gctcatcagg tctttgtagg tctcgatctc atcggcttcg aagggaacga
     8761 ttttggccac ggtttcatta ttaaacgtgt tcgccagctg cagatagaga ttggaaatct
     8821 cggcggcgta gatgccccgc cgtaacggat cgtttttgta gagctcgaac ttttttctca
     8881 aggcggcgac cgcttccagg agcgctgttt ggcgaacttt cgtatcatca tcgcgaccga
     8941 cactgaattc gacggcgccc aattgatcca ccagcacctg cgcctcctcg cgggcatggt
     9001 ccacgctggt ggcgagctgt tcgatgtcat tggtggcgga ctcggcggtt tccatcagct
     9061 gcttgatcac gttgtcgttg tcccggagga aggtgatcac cccgtcgatg ctgcgattca
     9121 tagagccctc cgttactgtc cgtcgtcttt cttcagtcga tccaggagtt ccaccagatc
     9181 agcggtgaat ctctgcgccc ggtccaatcc gccgacgcgg aggatgcgcg cgccgacaac
     9241 ttcttccagc gcatcgttga ccgttcggga atggttgagc acgctcatga tttgctcaga
     9301 ccgtcccggg gcttgatcga gaatcgccgc gacgaattcc ggatcgaccg aggctagcgc
     9361 cgattgtttg gcaaagcgtt ggacttcggc atgaagcgtg gcggtgtgtt tccaggaatc
     9421 gttgagcgcg ttggcggtgt cgcgcagggc gcagaagtcc tcttcaaatt gatcccacac
     9481 cgccttctcc agaccttgta cgtcggtcat gtagatcagg cttgtcaggt aggccgctgc
     9541 atacgccgct tcgcctcttt tgttcgtgag attccggaag gacgcgaggg caaagcggtg
     9601 cttggcttcc tgtatttgga gagacttgaa actctcatgc agtgaacttc gcgcctgctc
     9661 gaggaatgtg atccgctcct cggtctcctt ttgaaacaga tcgatattgg tttgggcggc
     9721 atcgatcttg gtcccttcca tctgaatcag atcacggact gaacggctgt gaatggaatg
     9781 gcagccggta gccatgagca acagcatggc cagggacagc agcgtcatgc ctcgtcggac
     9841 catggaaggc tccttccgcg gaacggtgta ggggccgctc atcggtgcac cgtgcgcagg
     9901 gcgtcggcca gcgattgcac gtcggatcgg ctcgcggtga cgtcgatgtc cagatattgt
     9961 ttgatcaagc cgttgattcg tcgaagcgcc tcgacattgt tccggtagcg gtcgaatgcc
    10021 gtgaggtcga cggtctgggg aatctttgca ccggctgcga cctgttgttc gacccgtgcg
    10081 atcaagtctg gcagttgctg gatgagggtg ctcatgtctc ctgatccggc ggcaaacaag
    10141 ggttgaccgt tcggcccttt ccgttccggg agcgatttca accgcatcgc attgaccaac
    10201 gtcagcacgt cggggccgag ggccgctgca tcatcctcag ccagtgcggc gtccggcacg
    10261 tccgcgacgc gggggttggt cgtcgcccat tgcagggcca gatcgagttt gagacgggtt
    10321 tggacatagc gataccactt cagatgacgg gtggtgacat tactcacgag ctcacggtac
    10381 tgctgcatga gctgctcatt ctccgcatag gcttggtctt tggccgcgag cgcctgtttg
    10441 atttcaggcg gggtggagca ggcggcgagt gaggcgaaga gcagcgtgcc ggcgatacgt
    10501 aacatcctca tcgggatgcc tcctggtaag cgtcgtccat tcgtatgatt ctggtgaaag
    10561 gcaatctacg tacccgatat aaatcgtgga gagtcgcgcc agagtagtcg tgaaatgacc
    10621 gacctgcaag gggaaaatca tgatgtgtcg aatcggcaca tcccgagcct cgtctctcga
    10681 ccgtacccat tcagatacgc gtgtatcgaa ccgcatacag cgccagctgc attccgcggg
    10741 cacgtatgtg ttggcgggtt acgagggaat gagggttttg cgtagagcgg caacggtggt
    10801 tgcatcgagg ccggccgact tgagttgttc atcggtggcc gaggcgatct gcggaaggct
    10861 gccgaattgt ttcagaaggc gtgaccgacg gatcttgccg atgccgggaa tttggtccag
    10921 ctcggatgac agcagcgcct tgccccgtaa gttccggtga taggtgatag caaagcggtg
    10981 cgcttcatca cgaatgcgct ggaccagatg cgtagcgggt gaggttggcc gcaatacgat
    11041 gggatttttc cgacccggca gaaagattcg ttcatctttt tcgccacgcg ctttagccaa
    11101 gcccatgatc gggatctgtt cctggccgac ctgtttcaat ccttcgagcg cagcgctcaa
    11161 ctgccccagg ccaccgtcga tcaggatcag atcgggccgg gcgagatctt ccgtcgcgcc
    11221 gtatcgtcgc atcaccgctt cctgcatact ggcaaagtcg ttggcgcctt ccaccgtctg
    11281 aatccggaat ttgcggtagt ccgatttttt cgcttgcccg tcctcccaca cgaccaggga
    11341 ggcgaccgat tgattgccca tgatgttgga aatgtcgaac ccctcgatgc gacggggcac
    11401 ggtgtcgagg cgaagcaggc gtttcagttc ggctgtggcc tggcgatcga gggcttcgtt
    11461 ccgcagatgg tcggcaatgg cggcggcggc attttcctct gcgagcagga ccagctggtg
    11521 ctttgcgcct cgttccgggg tgaggagtcg aaccgtgtcg ccacgtttgt cactgagcca
    11581 ttgctcgatc agcggggcat cgaccagatc ggtcgggacc aggagttcct tcggcggctg
    11641 cccttccttg ttataaaact gctcgatggc agaacggacg agttcctcat caggggagtc
    11701 ggcggattgc ggccagaaga aatcctttcg cccgatcagg agacctccgc gcacgaacag
    11761 gagctgaagg tcggctgccg tgccctgccg tgcgatcccg attacgtcct gatctgtggt
    11821 cgtgacctgg gtaatccgct gtttctcgag cgtgcgttcg acgctaaaga gacggtcccg
    11881 caggcgggcc gcttcctcga attcctcccg ttctgccgcc gactccatag cctgacgcag
    11941 actgtcgagg agttcccggt cccggccttc aagaaactgg cgaacctgtt tgacgatcac
    12001 atgatagtcc tcacgggact gattgccggt gcacggtgcc atacaccgtt taatttcaaa
    12061 ttcgatgcag gcccggtcgg cccgtccgtc gatgtcgatg tcgcacgtgg ccagtggaaa
    12121 cgccttgcga atgactttga gcgtctctcg gagcgcgccg gccggcgtgt agggcccgta
    12181 atacagcgcg ccgtctttct gaacgcgccg gacaatggag aggcgcggga aggattcttt
    12241 gatcggcaac cggagatagg ggtactgttt gtcgtcgcgc aggacgatat taaagcgcgg
    12301 gcggtggcgc ttgatcagat tgctttcgag gatcagcgct tccagctcgg agcgggtgac
    12361 gatggtttcg aggtcggcga cttcgctgac cagcaggctg gtcttggggg tctggtcgct
    12421 tcctttttgg aaataggagc gcactcgatc tgccagcacg gcggctttgc cgacatagag
    12481 aatctctctc ttcgcattcc ggaagagata acagccgggc tgttctggca gatggtccag
    12541 tttggattga agggtctcgc acgatccagc catgagaagt cagccttccg ccaccagcga
    12601 ggaacattgc aaccacgtcg ccagtaattc taacggtcgt agcaaatgac ctgcaacgtg
    12661 gtgctcgcgc ggacgaccga tcgtcgatcg ctggttagcg aagcgttcgc acgaaggacg
    12721 gactgaggct tcctctcgca acctccgcca ctggtccctt catcgtcaga ctgaaatcct
    12781 tggcgacctc gatattgagc gttccccccg gcatgatgac cttcaccgga cctttcacca
    12841 gtccgagctt gacggcggcg ctggctgccg cacaggacga ggagccggaa gcctgagttt
    12901 cccctgcgcc gcgttcccaa atcaggatga agatttcttt tggcccggtg ggcacggcaa
    12961 gttgaacatt ggtgcgtttt ggaaacagag gatggttttc caaggccggt cccagtgtga
    13021 ggagttcttc acgcgtccag gattgaccgg cttccttgaa gaccacgcag tggggattgc
    13081 cgacgctgac acccgtaaat tgcaatgacc gcccggcagc ctcgatgggc gcctggatca
    13141 attcctcgac ggacaacgtg cagggaaggg ctgagggttg gaaggtggct cggcccatct
    13201 ctaccgtggc cgccgccgca tctccatggc gatcgacatg caaggtgatc gaaaccagac
    13261 cgccctttgt ttcgaccgtg aacgcgcgct ttttcgtttt gccggtggcg tgcagatagc
    13321 gggcgaagat acggagtccg ttgccggatt tttcggcttc gctaccatcc ggattataaa
    13381 tgcggagacc gaaatcggct ttcttcgacg ggaccagcgc gaggatgccg tcgctgccga
    13441 gcccccaatg gcgatcgcaa atgccgcgga ttttcccggg cgtaaggttg aagctgagtt
    13501 ccttgggatc cattactaca tagtcgttcc cgagtccgtg cccgcgaaaa aacccgttct
    13561 tcatctgcac tctccttgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 gcggtacccg gcgggcgctc gatgagctcc acttcataac cgtccggtgc gtcgataaag
    13741 agaaaacggc tgccggatga cgtaggggtg ggaccgtcgg taatccggac gcccttggac
    13801 tccaggctcc ggatggtttc gtccaggttt tcgacctcga aggccaggtg gaccaggtct
    13861 tcctggacag tcaccggtcc gcttgcggga aagctgcaca gttcgatgag ttcgtcgctg
    13921 ttgggaactt ttagaaacgc caaatgcgat ccgcgcggag acactttacg gtccatcact
    13981 tccaagccta agacgttctg atagaagcgg atggtctgat ctatgtcgct gacgcgcatc
    14041 cgcgtgtgga gaagcttggt gactttcatg agtggtaccc cattcactgt ctgcggaaca
    14101 tggtctccat ctgcggtttc aatggtgtga cgccatttcc tcgccgggcc ggccgtcttg
    14161 cggaggagaa tttcggcgta tccgggaatc agaaaatttg gcatgggtcc gatttccttg
    14221 tagccctgct tgcggtagaa ggcgcgggcg gcctcattga agtcggatac gcaggcgaaa
    14281 agattctggg ctcgcgcgaa cgtgagcgat tcgatgtggg agaggaggct actgccgaat
    14341 ccacgaccgg tcgctgaagg cgcgatcccg agtaattcga gataatcgcc gaacagaaac
    14401 tttctgcgca cgatcgcaat ccccagcacg gctccctcca cctccagaat gaaggtgtcg
    14461 cgcccggccg gcagcggcgc gaagatgcgc agccagtccg aggccgcaaa ccccaggcgc
    14521 ttccaggggt cggagttggt caggatggcg atcacggcat cgcgatcctc ttcgcgcatg
    14581 tcgcgaatca gggcttgggc gcgactgtcg attggttgca tcgcagaaga tccgatatgg
    14641 tcattggagc gaggcccttg tcaggcagga gctgtccggc cagggcctcg gtcacttcac
    14701 gagtatgttt cccttttcca ttggcgtgca agacgatgac gctgcccggt ttgagtcgct
    14761 tctgaacacg ggtcaggatc gcatcggcgc tcagcgcggg gtcggggtcg cccgattcga
    14821 tattccacag aatgaaacgc aggccgagtg ctttcactgc gtcgactgtc tcgtcattgt
    14881 attccccata gggtggccgg aacagcgtgg ccggccgccc gtatttagtt ttgaggagac
    14941 gcaccggtcc catgatttcc tgttgctgct cctcgacatc atgcatcggg agatgtgcat
    15001 gcacgtcgcc atgggtgccg atctcgaaaa agggaacggc taagagctgc ttcacttcgg
    15061 catcgtgttt gatcatccac cgaccggaca tgaaaaaggt cgtcggcacc ttgtgctcaa
    15121 tgaggtagtc gatcaactcg cgatcgaacc ccgaagcggt gcgaacgggg cacagatcat
    15181 aggtcagtgc cacggcaggg caggaagcag gaccggattt gatgacctgt gcggacgacg
    15241 gccgggcctc tgtgatgaag accaagccgc agatcgcggc gattcctatc cagcagtctt
    15301 tcaagcgcat gccgcagtat acccgatggc acgcacctgc gatagttgac gctttatccg
    15361 ggtggctgct acggtgaaac catgcagggt ccagattgga aaatcggccg catgttcggc
    15421 attcccatcc atgtgcacgc ctcctggctg ttcgtgtttt tctttatgac ctggtcgctg
    15481 gcgacgggtt atttgccgga cacgcttccc ggcttatccg agccccgcta ttgggcgatg
    15541 ggcggcgtgg cggcgctcct cctgttcgct tccgtcctgc tgcatgagct cgggcattca
    15601 ttggtggcgc tgcggtaccg catcccgatc gggcagatta cgctgttcat tttcggtggg
    15661 gtggcgcaga tgcgaaaaga acccccccat ccacgggcgg aattcctgat cgcgatcgcc
    15721 ggtccgctgg tgagctttat cctggccgga ctttccctcg tactggtggc ggtcttggaa
    15781 ttgtttccgg ccgagtcgtc tctgcatggc ctgatggcat tgggcaccct gctcggcatg
    15841 gtgaacactc agttgggtct gtttaacctc ctgcctggat tccccttgga cggagggcgc
    15901 gcattgcgtg cgggattgtg ggcctggagt aaggattatt atcgggcgac aagccaagcg
    15961 gccctggttg gactgctgtt cggggtcacg ttcgcgctct tcggggcgtt actgctgttc
    16021 ggggctcttt ccggcacggc ctccagcgcc ttggcgagta gcggcgggtg gattgtgctg
    16081 ctgggcgcgt ttctttttac ggcggcgcgc ggtacccgca aacaagcagc cattcgagct
    16141 tcgcttgcgt ctgtgccggt tcgggaactc atggtccgga atgtggtggc gctttctcct
    16201 gatctctcgc tggaggaagc ggtgaaccag tacttcctgc cttacggcta tggagggttc
    16261 ccggtggtgc atgaggaccg ccttttggga gtcgtggcgg ttcgtgatat tcaatcggtc
    16321 aaaaattctc tctgggcctt tcgccgcgtg gccgatatca tgcagcccgc cagcgacgat
    16381 atggtcgtct cgccggatct gtcagcgatg caggcattgg agcagatgat agcggtgggg
    16441 gccgaacgac tggttgtggt ccaggatggt caattgctgg gactggtgac gcgcgcatct
    16501 atcggacatt ttatcgagca acaccatccg tcgggacttg gcaagtcttc ttcgtaaccg
    16561 gacggtttcc ttccctcaca ttccgcataa atagccatcc actgaggcat gtgagacctc
    16621 aacccgcgta actcatccgg gaagcgtcgc cgactcgtcg aggagggctg ggcgtcggca
    16681 ttggcattcg taggtcacca cggctcccag gaggaccagt gccgatgagt agtagaccca
    16741 cagcaacagt aagacgacct caagcagtga accatacagt tgcgtgtaga cggccgcatc
    16801 ttccaaatag gtgacgaaga ggaccttggc ggagacccag aggagactga ataccgcgcc
    16861 gccgatcgtc gcttcgcgcc aggttggccg ctggtgagga agaaagcgat acaaacaggt
    16921 gacggaggcg aaggccagca ggaatggcaa tgtataggtg atgaggaagt catgggccgc
    16981 gattgctacc agattcatgc cgagaaagcg cggcgcatag gtagtgagta gttcgatggc
    17041 ctgtgtggcc acgaaggata tgagcgtcag aaaccccagc atccagatca gggctacggc
    17101 gaccagcgtg gaaattaatg gatgccgttt ggctgccgtg ccgaacacgg tgttcatggc
    17161 atagtccaat tcgtagaaca ccagtgcgct gaaccatcca aaggccagaa agacggccca
    17221 tcgtacttcc tcctgctcag ccacccgccg gatatccttg gccagccgtt cgcccatgct
    17281 ggggaggaat cctttcagaa acccgagcag aaactggtag ccgatcacat cctggctcac
    17341 gatgaaactg atgccgtaga gcagcaggaa gaccatcggg aagagggaga ggatcgagta
    17401 gaacgccaac gaagctgcca ggctgggaca gccgcgacgt cgaaaggtgt cgaacacgtt
    17461 ggagagaaat gtgagttcag tcatgcagac cgcgcgattc agactcagtg cagagcggtg
    17521 aatgcttcgg atcgttgaga ggagccggac aaggcacggt ggcacatcat cgggaaatcg
    17581 aggcgccggt caaagtggcg tgacccctgc gcctggaggc tatttcagca acggtgccgc
    17641 atgggtggcc agcgaccaca gaccgttggg gaagagaccg agaaagacga cgccggcaag
    17701 ggcacaggcc aggacaaagg acagcgcggg agaggtttcc agccgcgagt gcgcagtggc
    17761 ggctccctcg ggctcgcgca tgtacatcac catcacaatc cgcaagtaat agaaggcgga
    17821 aatagcggcg aagataaccg cgacgaccgc cagccaggtc atgccgccgt tcacggcggc
    17881 catgaaaata tagaacttgc cgataaatcc agcggtcggg ggaatgccgg ccagcgagac
    17941 cagaaacacg agcatgaaaa acgcagccag cggttcgcgt ttggccagtc cggcataatc
    18001 ctcgatgttc tcgctctcct gtccttcttt gcgcaacatg ccgaccaagg caaaggctcc
    18061 cagggtcatg aaggaataga cggcgatata cagtaagacg ctggcgaaac cgctggacgt
    18121 cccttcgccg ctgtgacggg ctgccacgac gacaccgatc agggcgtacc cggcgtgggc
    18181 aatactggag tacgccagca tacgtttgat gctcgtctgt acgatggcga ccagatttcc
    18241 taacgccaag gtggcgagac agatgatggt gaacaggact gaccaatcgg cactcgcgcc
    18301 gcccaagcct tccacgaaca cccggaggaa cgcgccgaaa ctggcggctt tggacgccac
    18361 ggccataaag gcggtgacgg aggtcggagc cccttggtag acgtccggcg tccacatatg
    18421 aaagggcacc accgctagct tgaatccgaa cccgacggcc aggagtacga gtgccagcga
    18481 caacatcgga ttggtagtgc cgtgcgtggt gatcgcctcg gcgatcaccg acaacctggt
    18541 gctgccggcc aggccgaaga gcagagagat tccgtacagc aagacgccgg aagaaaaggc
    18601 tcccagcaca aagtatttgg ctgacgcttc cagcgaacgg gcttccgttc tcttcaaccc
    18661 ggccatcaca tagagggaaa gggacatcaa ctcggtgccg agataaatgg tcagcaggtc
    18721 ggcgccggac accatgacca tcatcccgac caaggccagc agaatgaagc cgtaatattc
    18781 gccgatgctg agtcgctcgg ctttgagata ggccagtgag aggagaacgg tcaggccggt
    18841 gacgatatac aacagcagct tccagaaggc gccgtacgaa tcgatcacca ccagattact
    18901 gaaggcatag gtttgaccgg tcaggtggga cgaggtcagg cccatgcaca tcgccagagc
    18961 gcccaacgtg agccaggcga ggacgtcctt tttgtctgct cccaggatcg ggtccaatgc
    19021 cagaatgagg caggccgtac cgatgacgat cagttcggga agaatggcca ggagatcttg
    19081 gagtggaaaa gtcatcgtgc cgctgcctgt tcagaagatg aggaagacat ggccgccatc
    19141 gttgctgccg gttgcgggag cgcaggttgc tgcggcgcag tcgcatatgc gggagccttt
    19201 tgtgcgttca agagattcgt cacgctggca tgcatacggg tcaacagcgg attcgggaag
    19261 atgcccagca cgaagaccag cacaattaag ggaacaagcg tggcggtctc tctcgcattc
    19321 aggtccagga gatgtgcgcg atgcgccggt gcgggcgtac cgaacacaac ccgttgcatg
    19381 agccagagga tataggcagc ggccaggatc acgcccaatg cggcgaaggc cgtcgcaaac
    19441 tgactccaga cgaaagtgcc ggccaaaacg aggaattcgc cgacgaaact gttggtgccc
    19501 ggaagtccca aggaggacag ggcaaaaatc atcaggaaca tggcgtaacg cggcatggga
    19561 ccggccagtc ccgtgttgtc gagaatctgg cggctatgag tccgctcgta gatgattccc
    19621 acgcacagga acaagccccc ggtggtgatg ccgtggttga ccatctgcat gaccgcccct
    19681 tcaatgccct ggatgttcag cacgaagatg cccagcgtta cgaagcccat gtgactgacg
    19741 ctggaatacg cgatcaactt cttgatatcc gactgcgcca gcgccatgta ggccccatag
    19801 atgatggcca cgatcgacag ggcgatcatt ggtttggtaa aggccaccgt ggcgtcggga
    19861 agcatcggca atgtgaagcg gaggaatccg taggtgccca ttttcagcaa cacgctcgcc
    19921 agaatgacgc tgcccgccgt gggagcctct acgtgcgcgt ccggcagcca ggtatggaac
    19981 ggaaacatcg gaaccttgac cgcgaaggcg gcaaagaagg ccaggaacag ccacatctgg
    20041 agcgacgcgc cataggtccc gcggctgagt tcaaggatgt cgaacgtatg cccgccctgg
    20101 aagtacaacg ccaggatggc gaccagcagg agcaggctcc cagctaacgt gtagaggaag
    20161 aacttgatgg ctgcgtacag gcgattcggc ccgccccaga cgccgatgag cagatacatc
    20221 ggaatcagca tgacttccca gaacacgtag aagagcacga agtcgagcgc cacgaacacg
    20281 cccaccatgg ctgcttccat gacgagaagc atggacataa acagctgcac gcgtttctcg
    20341 atggccgtcc aggacacggt gacgcaaaac ggcatgagga atgtggtcat cagcaccagc
    20401 ggaaagctga tgccatccat gcccaggcta tagttcaccg gcggagaact gatccaggaa
    20461 gcccgttcca cgaactgcat gttggcggtg gagctgtcga acaaccacca gagcggcaag
    20521 gagatgagga aattgccgac ggtgaagccg agcgccaccc aacgcgacga ctcagccttg
    20581 accaggaagc agatcaccgc tccgatcaac gggaaaaata cgatgaggct caaccaggga
    20641 aacgacgtca cgaaaatcta ctccacaact gaaacacgaa gcctaagaca gcgtaccggg
    20701 cgtcagggat tccactcgct ttcaactaca gcagcaaata caccgtcaag atgaccaccg
    20761 cgcccaatgt catgcccagg gcgtaatgtt gtgtctggcc gctctgtgtc agacggatca
    20821 accagccgcc ccaggcaacc gcacgggcga ctc
//
LOCUS       DAOQEW010000050        20759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_636_length_20759_cov_8.962568, whole genome shotgun sequence.
ACCESSION   DAOQEW010000050 DAOQEW010000000
VERSION     DAOQEW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20759
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_20759_cov_8.962568"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1742)
                     /locus_tag="PLS35_07545"
     CDS             complement(282..1742)
                     /locus_tag="PLS35_07545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI18550.1"
                     /translation="MEQHLMTPVEDPDALPQPIGEYKPVDYWQAHINTLFYQLRGDQQ
                     RSFYQTFASADYRLAHALAADYFEQAVKRDKKAAAKSSASGDAASRPSLTVMEWGPGN
                     GNLAACFLSHLQKLDKAGQIYPRVRYVLVDSHAPALERARTHPDLIPHLSKVESLCAD
                     VDNMSTIADGTVDRIICNEMWNELATKLVVKKGGEYEEEHLRPNLNERKAAAIADWSA
                     FVRAFDAKDIEKLKQFPPFLDDLIWEREYHKVDWKDVPYRKTLTEFMKAIDEEVLVPV
                     NLGAFGSIKEAKRVLAPDAVGFSSFDAGTADMQVLNDPEKPCYGQFGGQYSFMVNLAL
                     IQAVAKHLGITTTIIETQREFVGSRLGTNVMTLMDLLACHPTVGSKVQPWQLDQLTLK
                     TIRTLNEHYESPYQRKIEFPLRSEMPAEEREAAQGTLLSMKPNGIPDTIAYVTEEELS
                     QAQGDLEQLGYEREAWLMALGAPPSPVEYYHFAFRP"
     gene            1931..2491
                     /locus_tag="PLS35_07550"
     CDS             1931..2491
                     /locus_tag="PLS35_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18551.1"
                     /translation="MLVCPILLFSGSLLWAEDLPLTRNVPIPDFQNRTESAKPYNFDA
                     PPEGMFRHIVLAEGFDEELGFQRTHEIVPVKPTERFSAGARPIFIVFELHQHYQSFQV
                     FGRCYPEEVAGVDGKVVVAEDAMYIALEDQTGYLKLFPTESGWKPGRYKVEIHAGEQI
                     NDMSLMGTMRFTITAASESEVPARSR"
     gene            2516..3346
                     /locus_tag="PLS35_07555"
     CDS             2516..3346
                     /locus_tag="PLS35_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18552.1"
                     /translation="MTAALPLGYTGLPPQMPGAWAFWRLPVERNVVALQWDILATILL
                     TATIQSLFGVGVLLFGTPILLVLGYDFITALTILLPISLTINLLQVSKDYRYIQWPFY
                     RQILTLSIPCVVLCLLLVTTLKLNIGSIVGLFLIVAALKSVYGPFNRCIESLVRYERI
                     YFTVMGIVHGLTNLGGSLLTAMIHSKPYDKDQTRATTAVSYGTFALFQLITLSMSLGP
                     SAIATSTTALYMAVGVSMFLATELLLYSKMSTERYRTTFAAFLFLSGTALIFKSLFIR
                     "
     regulatory      3462..3536
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3558..5249
                     /locus_tag="PLS35_07560"
     CDS             3558..5249
                     /locus_tag="PLS35_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18553.1"
                     /translation="MNRSPATTPPYPQYQLTEVLRDIFAHLGMLLRLERSILGIIASY
                     AVAIGFFLLCVPIAVQELVSTFSFAMEPRMIFTLALFVASSLTGVAAFRVLQARAVET
                     LQQRIYTRVAIAFTRLLPQLRDDTFATPQAHRFMEADLLTRALVAMVADLFNVAVVGT
                     IGMTMLILFHPYFVLYILVLILGFVGLLTIFGRGGFFITLEMSRLHYEIFGWIQNISH
                     NLPHLRAAGDSPYLLERTDTLTRIYARIRQRRSDTLTGRQYKAAALWQVVGHSGLLIT
                     AGLLVVDGQLTVGQFAAAEVLVGNLLLNMDTLARRMVAMFFTFVSCREMAAVFSLPTE
                     EDGAKEDVPATQFGESGIHLTCRNLSYRAQDGLPIFQNVSLEVAPGENVAILCGTNRA
                     KTALAKVLAGLHPPSTGFVRYNDMNLAEVRRDSISRVRGLILDSHPTLLDGTLEDNIT
                     LGRPAIEYQDLQWALRFVELDHDIDAMPQGLHTPVSSGQTHLSMSQILRLLVARAIVT
                     RPQILIFDGTLHNMLPATRDLLLRRLCAKDEPWSVIFVSNDPTFSTHVDRQVSIE"
     gene            5382..6158
                     /locus_tag="PLS35_07565"
     CDS             5382..6158
                     /locus_tag="PLS35_07565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI18554.1"
                     /translation="MTVATRPWASVVIPIKDERDNLVPLTEQLVKVLDGREESRSAPF
                     ELIFIDDGSSDGSSDVLDSLAARYQTVKVFHFDRNYGQSAAFDAGFKRSTGELVMTID
                     GDLQNDPADIATLLPHINTFDLVCGWRKDRHDNLTRKISSRIANSVRSAVTGDRVHDT
                     GCSLKLFRRAVVEKMQLFEGMHRFFPALALMHGFTVTEVPVRHYPRTRGTSKYGVGNR
                     LFKGLYDLVAVRWMQHRCLRYHYRGATTSPSGSTSASVPL"
     gene            6155..6454
                     /locus_tag="PLS35_07570"
     CDS             6155..6454
                     /locus_tag="PLS35_07570"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNI18555.1"
                     /translation="MTLDTIWLIIGFLGQGIFFMRWVVQWIASERHAESRVPTAFWYM
                     SMIGGLITLAYAIYRQDPVFIAGQSIGSIVYLRNLMLIHRPNSADSSTASPAIKS"
     gene            6466..8247
                     /locus_tag="PLS35_07575"
     CDS             6466..8247
                     /locus_tag="PLS35_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNI18556.1"
                     /translation="MNGEVPSLQTPAPIDRSIQPVTLVVLLALAAVLFFVGLGSLGLT
                     DRDEGRNAEAGREMFETGNYISPTFNYEPRFAKPVFVYWLMTASYHLFGVSEFAARFP
                     SALFGLGLILLQYLFLTRCRGPVIGLFGAAMLLLNLEIIGLSRMALTDSVLIFFTTLS
                     LYGFWLGLYGEGRERHCIWLFYIGMALATLTKGPIGFLIPLLAVGLYLWLTRSWPLFR
                     REGRLLPGLFLFVALALPWYLMMLNLHGQRYTTSAQGDTIGRFFGTMEGHGGTLLFYL
                     PVFILGFFPWSGLLPFAWYQAYRSWREAKQAGTFTPATGTDTPASSQALEWFAAAWVL
                     GGLVFFSLSSTRLPHYIGPLFPAAAILTASFWNRCVTDHAAPHLRAAIHMMTAVGYIL
                     ALAFASLPPLYAKFAVKLVDEFPLAGQVTLGPGPYTVASIFLVGMGLVAYFGLSETRK
                     PAAFWAAGGSLALVVLAVTQLTFPLVNHFVIEPPQRLADVAGLNLGPDDRLILYGQPR
                     PSLVFYAKRKAIVVPKGEEANIKPYLTQPGRTLILLPAAMRNRLPFETMEYPVMLERY
                     GYILLANQSMIHVPEEAEKPPIRIPGH"
     gene            complement(8255..9334)
                     /locus_tag="PLS35_07580"
     CDS             complement(8255..9334)
                     /locus_tag="PLS35_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNI18557.1"
                     /translation="MSESGDGQAAPVSTQPAPPPDRPPVLAVILSALVVLGSFAALFH
                     IDIPILRLLRSHNLSSLQSLGDLGEKLGNGGTLITISVLLLGAGYLLKRQAWIRTAVD
                     SLLAHGVVALVVNSLKHIIGRPRPRLTHSGGWHWWPSLQSGLDSFPSGHTSATVAVAT
                     VLARVLPRLSWVPFALAAWVGASRIWRGSHFPGDVIGGMVLGFMVGSIFNGPLRWWGS
                     SCAHALVRIAPMVLLLTGWCWVVTHRILDPVTDTVLVASGVMLVLAGWLVQMLPRWRS
                     TSAGGIAAVVTSKGFLLSGVGLATGAPIVIVLTGLLCIARWNAAAQVLEAPAQPSWRR
                     DGLYTAAGLAGVMAIQFLKGLVPLE"
     gene            complement(9331..10458)
                     /locus_tag="PLS35_07585"
     CDS             complement(9331..10458)
                     /locus_tag="PLS35_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18558.1"
                     /translation="MKHAKQVMAGMLGLMLSLSMTNPAAAASKAAETATPTASTERHL
                     TNIKQLTFGRQNAEAYFSFSGDKLIFQSTNNWMKDTFAAAMHPADIPLGCYQMYVMDL
                     GSEKIRMVSTGSGTTTCGYFFPGDRRVLYSSTHLRGPNCPPKPKREGGAYRWALDDYD
                     LFSVRIDGLDPQRLTNTPGYDAEATVAPNGKTIIWTSMRDGDLDIYAMDLDGTHPRRL
                     TQEIGYDGGAFFSPDSKRIVYRAQHPSNQEELDQYKSLLAQNLVEPGRLEIFIMNADG
                     SNKQQVTSNGASNFSPYFHPDGHRVIFSSNVETRNEGGRPEFHLYLTNEDGSGLERVT
                     VGGKFNSFPMFSPDGKHLVWVSDRNAKEPGEFNVFLADWVP"
     gene            complement(10445..12625)
                     /locus_tag="PLS35_07590"
     CDS             complement(10445..12625)
                     /locus_tag="PLS35_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNI18559.1"
                     /translation="MHRLIRFSLLAGCLVPALAVAEPVSSLPTITHHQLTLQLLPDSH
                     QLIATDRLILSGRASGQALSFSLAPTLRVERIQDVTHCEAACETAPELAFERDGSAGP
                     SEPQRVIVKQPATDRGYGAVALKFSYRGVVDDPPRDPRHLRFVTPSETSGHIGPEGVY
                     LSSESQWYPDVEGSLATYDVTIALPDGWTAVTQGAPQSDQGHWIVPTKSEALTVVANR
                     FVVKSRTWTAKSGQSIRLATYLFPDEASLADEYLDASARYLDAYIPLLGPYPFSQFAV
                     VENFFSSGLGMPSFTLLGSGVIKRHYTQPYALGHEIVHSWIGNGVFNRIDRGNWVEGL
                     TTYLSNYYYHELMGDEAQAREQRRLMLMGYAVYVRPGEAYPVIQFAQKHDEKDNAIGY
                     QQAAMVFHALRQEIGEAVFWTALKQIPERYLGMAAEWRDLERVFQEAAGRDLRWFFAQ
                     WVERAGVPDIEVTGSRVQQSTGTAGDMNRVEAIVHIAQHGTPYRLAIDLEFVLDGGRT
                     HRTRIHLTDAQQNAIIPLPERPLAVRLDPDLHVFRRVARNALAPMLNVYVTDAHRTVV
                     VAPASLQQPGPFTDIVQRIAAQEASKPEPSRTTVKQPEDKTKPTPEGSVLVLGGPGEN
                     AAASDMLRYCGDHVHFTEKGFSVEGKSYEGPTMALLVSCRRDDQPGSVVTILYGVTPP
                     ALTRVARLLFFYGWQSYVVFHEGSVVARGDWEDRMTVEVRIETR"
     gene            complement(12668..13453)
                     /locus_tag="PLS35_07595"
     CDS             complement(12668..13453)
                     /locus_tag="PLS35_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18560.1"
                     /translation="MTTLLVIDDDRLHCDLLQVALSRHGYRVSTATSGREGVAIFRQL
                     RPVVTLLDLRMPEMDGLAVLKEIRTYDPRAGVIILGGGATEELENLARKLRVTDFFRK
                     GLSLDVLVGAVHRIAQQARREASGTQDVTASEIDQPSEEQILVVDDDVMARDLLMRFL
                     SLRGYRVRGAKDGREALRMIEESAPDLLILDLVMPEMNGVELLKVLATREYAGRTIIL
                     SGHQNDPLLEDAWALGPQEVLDKPLDLERALMAIQLVMVCREC"
     gene            complement(13604..14518)
                     /gene="ftsY"
                     /locus_tag="PLS35_07600"
     CDS             complement(13604..14518)
                     /gene="ftsY"
                     /locus_tag="PLS35_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNI18561.1"
                     /translation="MGWFQKLSEGLSKTRNAVTGQLDRLLGRAADPALLEELEVALIS
                     ADLGMPVVERVMENLRAQMRGGNFSAAEKVRELLRQSVLDILLPTQGASIDQLIAQGP
                     RPFVILAVGVNGVGKTTTVAKLTQRFRQQGNTALLVAGDTFRAAAIDQLQVWADRTQV
                     DIIRHRPGADPAAVAYDGITAAKARGSDVVLIDTAGRLHTKTNLMDELRKIKRIVAQE
                     CPGAPHEVLLVLDATVGQNAIAQARQFHEAVGVTGIALTKLDGTARGGIVVAIADTLK
                     IPVRLIGVGESVEDLQDFNAKAFVDALF"
     assembly_gap    14530..14629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14630..15555)
                     /locus_tag="PLS35_07605"
     CDS             complement(<14630..15555)
                     /locus_tag="PLS35_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNI18562.1"
                     /translation="MRKIKLREGTNTAVLFGHHDRHLKLIEEEFGVRCSARGEEVTVE
                     GAADAVKQAERVLNELASLTNEGYELRSDDVTHALTALRHNHDASLKELLFSASPIVT
                     RKRFIVPKTPTQKYYLDAIEKHDIVIGIGPAGTGKTYLAMAMAVSALMKKDVSRIILA
                     RPAVEAGEKLGYLPGDMYAKVNPYLRPLYDALFDMMDMERANRLIERGDIEIAPLAFM
                     RGRTLNDSFVILDEAQNATAEQMKMFLTRLGFHSKAVVTGDITQIDLPSDRVSGLIEV
                     RNILQDIAGIEFVYFDERDVVRHRLVQEIIKA"
     gene            complement(15681..16457)
                     /locus_tag="PLS35_07610"
     CDS             complement(15681..16457)
                     /locus_tag="PLS35_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HNI18563.1"
                     /translation="MADDPDGTEPTKRFQWIVNSAPVLGATGLELDPVHVKRGDQVSL
                     EVVAFDGELESAPYRTEPVSVVNTPPLVSHVTVEADAEHNSRVLAKVDAVDPDRDEIH
                     YNYRWWRNDKQVKEGEESILDTTGFGRKDVVVVEVIARDQDAAAAPVRSAPSALGNSP
                     PLIVSNPAALTNREQYEYVVQAKDVDGDTVSYVLETGPPGMTIDRATGQVSWKVIPGV
                     AGTHRVKIMAEDGQGGIAWQEFELSIPSTAQSQGSLPPQG"
     gene            complement(16700..20614)
                     /locus_tag="PLS35_07615"
     CDS             complement(16700..20614)
                     /locus_tag="PLS35_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18564.1"
                     /translation="MKTVRAQATGVNDERADVNYSGGTGANTYVGRIPLADRGSNPGT
                     ISIGISASGTPAVPYFCVDNDTSFNSNCGDSYSVRKYELKVGYSTEPTGVIQQIGSKA
                     RFGLVEFKSSSEGARMLVGAGSRQSIDWNGTGVETFNTNMAAMVDAVQESYPSTWTPL
                     SESLYETARYVAQIQSTFVTGYVYPIAFSGGISNGVNFATTGAGSIGTDEITALVGTE
                     TCPAGYITNACGRDPYFFGQNHTPPWASTSQVVNCCRTFVIIFTDGEPTQDVNVPTAI
                     QDYAHAHHGQHCNGSDSAAPPRPINGTCNTNAATPFSDLLAEHKTDYADSGSHYLDDV
                     AYWAHTSDLRPCSGTSDGTIAGLSVTGHCIPGTQNLTVYSFFAFGNINGRGILAQTAR
                     LGGFEDANGDGIPQTSEWDKENNLTGASTPDGIPDAYFESSNVDDLQDKLLATIASIL
                     RRSASGSSVSVLATASTGEGALYQSYFYPSTIEPSTLSDVKWTGYTQALFIDTFGNTR
                     EDTNQDGRLDYKVDKIIKTRFDSLSNSVKVDKYVDSDGDGLPNDQNSDGVVTLADCNP
                     CGKLLSDILPIWEAGKQLALKDASTRTILTWVDSDHDGVVDSGEQIAFSTANETTLRP
                     YLRATSSAEGTKIINFIRGCDATTCTDQATTRDRRLQVPSGSGTLKVWKLGDVIHSTP
                     TVVAGPRDRHDAIYGDQSYTAFLQKWYNRRQVAYVGGNDGMLHAFNAGYYHPGDDTST
                     SAPASTTEHGWFTTAPADNSSGAPLGDELWGFVPYELLPQLEFLSRADYQHAYYVDLP
                     LKVADVRIFTADTDHPNGWGTILIGGFRMGGSCGACVAGTGAPPMTVNIGGTNYNFYS
                     AYFAMDVTNPDSPRLLWSFSSSDLGLSTSVPAVVRVNPAADAKASNTNAKWYMVVGSG
                     PTGYDASVAQGAKVFAIDLATGPGSSNSLVTSYSIGSWDSYIGDMTAFDRDLDYRHDV
                     VYFGRVINDGALPWRGKIYRLTMGAGGATSKFGTVTSPSQWGISSGSARVPTEMLDTF
                     ASGAEMGPVASAPSVTTDDAANVWVFVGSGRYYSKSDKTDISTQYFVGMKDSVLNAGC
                     NQSSGITACMDSDLVDVSNATVCVVGVGDCGLATGTNQVTGVTGATTFTGLIGLVQSK
                     DGWVTKLVEPANPPTKPVPYGIGERAVSSPTVFGGVVFFPTFIPTNDICVSSGTSRLW
                     ALFYKTGSAYQEPILGTTGAGANQTVNKFGSLGEGLAFGVVVHMGSGRDGGSPFGLLI
                     NMSQGNFGDCATCTTGGGMFGSNISVPVAIDPRSRFFSWTNQ"
BASE COUNT         4274 a         6298 c         5604 g         4483 t
ORIGIN      
        1 cgaaccaaac atggtactgt atgctccttg ttacagctgt gcgattattc gcacgacata
       61 tgttcacgca ccggcgactc gactgccact cgtgaagtac gtcgtctgag aagatcttgc
      121 ctgcgaaccc catctccttc tgaatatttc ccacgcgtgg agatggagag gtcttactat
      181 cggaaatcgc gggaaaggct atagaaacga ctggaattag tcaagctctt tctcctacag
      241 gtgccccctt acgtctcttt ccctcctgcc cgaaccagcg actacggtcg gaacgcgaaa
      301 tgatagtact caaccggact cggaggcgct ccaagcgcca tcaaccaggc ctcccgctcg
      361 tatccgagct gttccaaatc accctgtgcc tgactcaatt cttcttcggt gacatacgcg
      421 atggtgtcgg gaatgccgtt gggtttcatc gagaggagcg tgccctgtgc cgcttcgcgt
      481 tcttccgcag gcatttcgct gcgcagagga aactcgatct tgcgttgata cgggctctcg
      541 taatgttcat tcagggtacg gatcgttttc agcgtgagct gatccaattg ccagggctga
      601 accttagagc ccaccgtcgg atgacaggcc aggagatcca tcagcgtcat gacattggtt
      661 cccagtcgac ttcccacaaa ctcacgctgg gtttcaatga tggtggtcgt gatcccgaga
      721 tgcttggcta cggcttgaat gagagccaga ttcaccatga aactgtattg gccgccgaac
      781 tggccataac agggcttctc gggatcattg agcacctgca tgtccgctgt tcccgcatcg
      841 aaactgctga accccacggc gtccggggcc agcactcgct tcgcttcctt gatcgacccg
      901 aaggcaccga gattgaccgg cacgagcact tcttcatcaa tcgccttcat aaactccgtc
      961 agggttttgc gataaggcac atccttccaa tccaccttgt gatattcgcg ctcccagatg
     1021 agatcgtcca gaaagggcgg gaactgtttc aatttttcga tgtccttggc gtcgaaggcg
     1081 cgcacaaacg cggaccagtc ggcaatcgct gccgccttgc gctcgttaag gttcgggcgg
     1141 agatgctctt cctcatattc cccccccttc ttcaccacga gcttggtggc caattcattc
     1201 cacatctcat tacagatgat ccggtcgacc gtaccgtcgg cgatggtcga catattgtcc
     1261 acatcggcac agagggactc gaccttggag agatgcggaa tcaaatccgg atgcgtgcgt
     1321 gcccgctcca gagcaggagc atgagaatcc acgagtacat accgcacccg cggatagatc
     1381 tgccccgctt tgtcgagctt ctgtagatga ctgagaaagc aggccgcgag gttgccgtta
     1441 ccggggcccc attccatgac ggtcaaggat ggtcgggaag cggcatcgcc ggaggcagaa
     1501 gacttcgccg cagccttctt gtcacgtttg actgcctgct cgaaatagtc ggccgccaac
     1561 gcatgggcca accgataatc ggccgaggcg aaggtctgat agaagctgcg ttgttgatca
     1621 ccgcgcagtt gatagaacag agtattgatg tgcgcctgcc agtaatcgac cggcttgtat
     1681 tccccgatgg gctgcggtaa cgcgtcggga tcctcgacgg gtgtcatgag gtgctgttcc
     1741 attctgtccc cctgtagaaa gagggaaagt ctaacaggaa gcaattttca cccgcaacct
     1801 cttccggacg aatccccctc agcattcatg atgcctttcg gtgaacgccc cgaccgagtt
     1861 ccgacgctgt atgccttcct tgacaggcct cccgggcgga gtgtaccaag acaggatgag
     1921 atcggcgtgt atgctcgtct gtcccatcct tcttttctcc ggctctctct tatgggccga
     1981 agatcttccc ttgactcgca acgttcccat tcctgatttt caaaaccgca ccgagagcgc
     2041 caagccctac aatttcgacg ccccgcccga aggcatgttt cgtcacattg tgctcgccga
     2101 agggttcgat gaagaattgg gcttccagcg gacccatgaa attgttcctg tcaaaccgac
     2161 cgaacgcttc tccgccgggg ccagaccgat ttttatcgtg tttgaactgc accagcacta
     2221 ccaatccttc caggtcttcg ggcgctgcta tccggaagag gtggccggag tcgatggaaa
     2281 agtcgtagtt gctgaagatg cgatgtacat cgcgctggaa gatcaaaccg gctacctgaa
     2341 attgttcccg acagagagcg gttggaaacc ggggcggtat aaagtggaaa tccatgccgg
     2401 cgaacagatc aacgacatga gcctgatggg caccatgcgc ttcacgatca ctgccgcatc
     2461 tgaatccgaa gtccccgcca gaagccggtg accaaacccg cacgcgtcag agcgattgac
     2521 ggctgctcta ccactgggct ataccggtct gccgccgcaa atgccaggcg cctgggcctt
     2581 ctggcgcctg ccggtcgaaa ggaacgtcgt cgccttgcag tgggatattc tggccaccat
     2641 cctcctcacg gcaaccattc agtcgctctt tggtgtgggc gtgttgctct tcggcacccc
     2701 gattcttctt gtgctcggtt atgacttcat caccgcgctg acaattctgc ttcccatttc
     2761 gctgaccatc aacctcctgc aagtcagcaa ggactatcgg tatatccagt ggccgttcta
     2821 tcggcagatc ctcaccctgt ctatcccctg cgtggtgctg tgcctcttat tggtcacgac
     2881 gctgaagctg aacatcggca gcatcgtagg cctgtttttg atcgtcgcgg cgctgaagag
     2941 cgtctatggg ccgttcaaca gatgcatcga atcgctggtg cgatatgagc gcatctactt
     3001 cacggtgatg ggcatcgtcc acggcctgac caatctgggc ggctcactgt tgacagccat
     3061 gattcacagc aaaccgtacg ataaggatca gacccgggcg acaacggccg tctcctacgg
     3121 aaccttcgcg ctgtttcagc tcatcacgct gagcatgtcc ctcgggcctt ctgccattgc
     3181 cacatccacc acggcactct atatggctgt aggcgtcagc atgttcctgg ctaccgaact
     3241 cttgctctac agtaagatga gcacagaacg ataccgcacc acgttcgccg catttttgtt
     3301 cctgtccggc acggccctga tcttcaagtc tctcttcatc cgatgaccgg caaggggtca
     3361 ttcgccagcg gcattgtcag tcgccaggcc tcgtcgcgta tccgaagggg ctttgtgttg
     3421 acgggaaacg ggctctcagt ctagactttg cccagccgta tattctctcc ggtgatggag
     3481 ttcaccgaga accgccgagt gcgatcctcg cctcggctga taactcctac cagcgcccac
     3541 gcggtaggag tttgtttgtg aaccggtccc ccgcaacgac accaccctat ccccagtatc
     3601 agctgaccga ggtccttcgc gacattttcg cccacctggg catgttgctg aggctggagc
     3661 gatccatcct gggcatcatc gcctcgtatg cagtggccat cgggtttttc ctcctctgcg
     3721 tccccattgc cgtccaggag ctcgtcagta ctttctcctt tgcgatggaa cctcgcatga
     3781 tcttcacgct ggccctgttc gtcgccagtt ccttgacggg cgtcgcggcc tttcgcgtgc
     3841 tgcaggcgcg ggccgtggag acgctgcaac agcggatcta tacgcgcgta gccatcgcct
     3901 tcacgcgcct tctcccgcag cttcgcgacg acacctttgc cacgccgcaa gcccaccggt
     3961 tcatggaagc cgatctgctg acgcgcgccc tcgtcgcgat ggtcgcggat ctctttaacg
     4021 tggctgtggt cggcaccatc gggatgacca tgctgatcct gtttcatccc tacttcgtac
     4081 tctacattct ggtgctgatt ctcgggtttg tggggttact caccatcttt gggcgggggg
     4141 gattctttat caccctagaa atgtcccgct tgcactatga gatattcggc tggattcaga
     4201 atatctcgca caatcttccc cacctgcgag cggccgggga tagtccgtac ctcctggaac
     4261 gcaccgatac gctgacacgc atctatgcca gaatccggca acggcggtct gacacgctca
     4321 ccggacggca gtacaaagcc gcggccctgt ggcaagtcgt cggccatagc gggctgctca
     4381 tcacggcggg actcctggtc gtcgatggac aactgacggt cgggcaattt gccgccgccg
     4441 aggtgctggt cggcaacctc ctgctgaata tggacacctt ggctcggcgc atggtcgcga
     4501 tgttttttac attcgtgtcc tgccgggaaa tggccgcagt gttctccttg ccgacagaag
     4561 aagacggcgc gaaggaagac gtgccggcga cacagttcgg agaatcggga attcacctga
     4621 cctgccggaa cctttcctac agggcgcagg atggccttcc gatttttcaa aacgtgtctc
     4681 ttgaggtcgc gccgggagag aacgtggcca tcctgtgcgg caccaacagg gccaaaaccg
     4741 ccctcgcgaa agtcctggcc ggtctacacc caccttcgac cggatttgtc cggtacaacg
     4801 acatgaatct cgctgaagtg aggcgcgact ccatcagccg ggtacgcgga ctcatcctgg
     4861 attctcatcc gacgctgctg gacgggacgc tggaagacaa catcacgctg ggccgacctg
     4921 ccatcgaata ccaggatctc caatgggccc tgcgttttgt ggagctggac cacgacatcg
     4981 atgcgatgcc acaagggctg cacacccccg tctccagtgg acagacacac ctctcgatga
     5041 gtcagattct ccgcctcctg gtagcccgcg cgattgtgac ccgcccgcaa atcttaattt
     5101 tcgatggcac gttgcacaat atgctgcccg ccacaagaga cctgctactc cgccggctct
     5161 gcgcgaagga cgaaccctgg tcggtgatct tcgtgtcgaa cgatcctacc ttctcgactc
     5221 atgtcgatcg gcaggtgtcc atcgagtagc ggctcccgat tcgcatggac cggcaacggc
     5281 actgaacgag acagaatcgt ttgaccaccc ctccatgcgt tttgtagaat gtgccccgtg
     5341 cacactgatc aacggctgcg taaaggaact gaccccgctc catgactgtg gctacccgcc
     5401 catgggcttc cgttgtcatc ccgatcaagg atgaacgcga caatctggtc cctctgacag
     5461 agcaattggt gaaggtcctg gacggccgag aagagtcgcg atcggccccg ttcgagttga
     5521 ttttcatcga cgacggcagt tcggacggca gttcagacgt gttggatagc ttggcggccc
     5581 ggtaccaaac cgtgaaggtc tttcatttcg atcgcaacta cgggcaatcg gcggctttcg
     5641 atgcaggatt caagcggtcg acaggagagc tggtgatgac catcgatggc gatctgcaga
     5701 acgatccggc ggatatcgcc acgctgctgc cgcacatcaa caccttcgat ctcgtgtgtg
     5761 ggtggcgaaa agaccgccac gacaatctga cacgaaagat ttcgtcccgt attgccaaca
     5821 gcgtccgcag cgccgtcacc ggcgatcgcg tccacgacac cggctgctca ctgaagctgt
     5881 ttcggcgggc tgtggtggaa aaaatgcagc tcttcgaggg catgcatcgt tttttccccg
     5941 cgctggcctt gatgcacggg ttcacggtca ccgaagtacc ggtgcgccac taccctcgca
     6001 cacgcggaac ctcgaaatat ggtgtaggca accgtctgtt caaagggttg tacgatttgg
     6061 tggctgtgcg atggatgcag caccgctgcc tgcggtatca ctaccggggc gcaacaacat
     6121 ctccttccgg atcgacctcg gcctccgtac cactatgaca ctcgacacca tttggctgat
     6181 tatcggattc ctcggacagg gaatcttctt catgcgctgg gtggtccagt ggatcgcctc
     6241 cgaacgccat gctgaaagcc gcgtgccgac ggccttctgg tatatgagca tgatcggcgg
     6301 cttgatcacc ctcgcgtatg ccatctaccg gcaggatccg gtcttcattg ccggacagag
     6361 catcggcagt atcgtctacc ttcgcaacct catgctcatt catcgcccga actcggccga
     6421 ctccagcacg gcttccccgg cgatcaaatc ctaatggaac ctcagatgaa cggagaagtc
     6481 ccgtccttgc agacgccggc gccgatcgac cggtcgattc agccggtgac actcgttgtg
     6541 ttgttggcgt tggccgccgt gctgttcttt gtcggactcg gctctcttgg cttgaccgat
     6601 cgggatgaag ggcgaaacgc tgaagccggt cgggaaatgt tcgaaacggg caattacatc
     6661 agccccacgt tcaattacga gccgcgcttt gccaaaccgg tcttcgtcta ttggctgatg
     6721 acagcctcgt accatctgtt cggcgtgagt gaattcgcgg cgcgatttcc ctctgccctg
     6781 ttcggtcttg gcctcattct gctccagtat ctgtttctga cacgctgccg cggaccggtc
     6841 atcggcctgt tcggcgcggc catgctgttg ttgaatctgg agatcatcgg cctcagccgc
     6901 atggcgctca ccgacagcgt gttgatcttc tttaccacgc tgtcgctcta cggattctgg
     6961 ctcggcctct acggagaggg acgtgaacgc cactgcatct ggttgttcta catcggcatg
     7021 gcgctagcca cactgacgaa ggggccgatc ggttttctga ttccgctatt ggcggttggg
     7081 ctctatctct ggctgactcg ctcctggccg ctgttccgtc gcgaggggcg tctcctgccc
     7141 ggcctgttcc tctttgttgc cttggctctc ccgtggtatc tcatgatgtt gaacctgcat
     7201 gggcaacggt acacgacctc cgcgcaaggg gataccatcg gccgtttctt tggcaccatg
     7261 gaaggacacg gtggcaccct cctgttctat ctgcctgtgt tcatcctggg cttttttccc
     7321 tggagcggcc tcctgccgtt tgcctggtat caggcctatc gaagttggcg tgaggcgaaa
     7381 caggctggga ccttcacacc ggcgacaggt acggataccc ctgcctcctc acaggccctc
     7441 gagtggtttg ccgctgcctg ggtgctgggt gggctcgtgt ttttcagcct gtcttcgacc
     7501 cgcctcccgc actacatcgg ccccttgttt cccgcagccg cgatcttgac ggcatccttc
     7561 tggaatcgct gtgtgaccga ccatgcggcg ccccacctgc gtgctgcgat ccacatgatg
     7621 accgccgtgg gctacatcct ggcgctggcc tttgcgtccc tcccgcccct gtatgccaaa
     7681 ttcgccgtga agttagtgga tgaatttccg cttgccggac aggtgacact cggaccgggg
     7741 ccctataccg tcgcctcgat ttttttggtg ggaatgggtt tggtcgccta tttcgggctc
     7801 agcgagacgc ggaagccggc cgccttctgg gcggcaggag gatccttagc cttggtggtc
     7861 ctagccgtca cccaactgac gtttccattg gtcaatcact tcgtgatcga accgccgcag
     7921 cggctggccg acgtggcagg actgaatctc ggaccggacg accggctgat tctctatgga
     7981 cagccacgcc cctcgctggt cttctatgcc aaacgaaaag cgatcgtcgt ccccaagggt
     8041 gaggaagcta acattaaacc ctatttgacc cagccgggtc ggacgctgat tctcttgccg
     8101 gcagccatgc gcaaccggtt gccgttcgaa acgatggaat atcccgtcat gctcgaacgg
     8161 tacgggtaca ttctgctcgc caatcaatcc atgattcatg tccccgagga ggcagagaaa
     8221 cctccgattc ggattcccgg acactgagcg cttttcactc taatggcaca aggcctttca
     8281 gaaattggat ggccatcacc cccgcaagac ctgccgccgt gtacagccca tccctgcgcc
     8341 aggacggctg ggcaggtgct tcgagaacct gtgccgcagc attccatcgt gcgatacaga
     8401 gaagtccggt gagcacgatc acgatcggtg ctccggtcgc caggcctacc cctgacagca
     8461 agaagccttt cgaggttacc acggctgcaa tacccccggc ggaagtcgat cgccaccgag
     8521 gcaacatctg cactagccaa cctgcgagca ccagcatcac tcccgacgcc accagtacgg
     8581 tatcggtcac cggatcgaga atgcgatgcg tcaccaccca gcaccagcct gtcagcaaca
     8641 acaccatggg cgcgatgcgc acgagcgcat gcgcgcacga gctgccccac caccgcaatg
     8701 gtccgttgaa gattgagccg accataaagc ccaacaccat cccgccgatc acatccccgg
     8761 ggaaatgaga gcctctccag atccggctgg ctccgaccca cgcagccagg gcaaacggca
     8821 cccatgaaag cctgggcagc actctcgcca agaccgtcgc cacggcgacc gtcgccgagg
     8881 tatggcccga gggaaatgaa tctaaccccg attgtaacga aggccaccag tgccatccgc
     8941 ctgaatgggt gaggcgtggc ctgggacggc cgatgatatg cttgaggctg ttcaccacca
     9001 acgccaccac cccatgggca agcaggctat ccaccgccgt ccgaatccac gcctgtcgct
     9061 tgagcagata cccggctccc aggagaagca cgctgatggt aatcaacgtc cccccgttgc
     9121 ccagtttttc gccaagatca cccaacgatt ggagcgacga caaattgtgt gagcggagta
     9181 accgcaggat gggaatatcg atgtgaaaga gcgcggcgaa cgacccgagc accaccagcg
     9241 cggaaaggat caccgcaagg accggcggcc tgtccggcgg tggcgcaggc tgcgtcgata
     9301 ccggcgcagc ctgcccgtca cctgattcgc tcatggaacc cagtcggcaa gaaacacgtt
     9361 gaattctcca ggctccttcg cgttgcggtc ggacacccag acgagatgct ttccgtccgg
     9421 agagaacatg ggaaagctgt tgaacttccc gccaaccgtt actcgctcca ggccggagcc
     9481 gtcttcattg gtgagataca aatgaaactc aggccggccc ccttcattgc gcgtctccac
     9541 gttcgacgaa aaaatgaccc ggtgcccatc cggatggaaa tagggagaga aattggacgc
     9601 gccgttgctc gtcacctgct gcttgttcga cccgtcggca ttcatgatga agatttccag
     9661 gcggcccggt tccacaaggt tttgcgcgag gagtgatttg tactgatcca gttcctcctg
     9721 attactcgga tgctgggcgc gatacacaat ccgcttacta tcgggcgaga agaatgcccc
     9781 gccgtcgtag ccgatttcct gtgtcagccg acggggatgc gttccgtcca gatccatggc
     9841 gtagatatcc aaatccccgt cacgcatcga ggtccagata atggtcttgc cgttgggagc
     9901 cactgtcgcc tcagcgtcat atcccggggt attcgtcaat cgctgcgggt ccaagccgtc
     9961 gatccgaacg gagaataaat catagtcgtc cagcgcccac cggtaggcgc cgccctctcg
    10021 cttaggcttc ggaggacaat tggggcctcg tagatgagtc gacgagtaca ggacgcgccg
    10081 atcaccggga aagaaatagc cgcaggtcgt ggtgccggac ccggtgctga ccatccggat
    10141 cttctcgctc ccgagatcca tcacatacat ttggtaacag cccaggggga tgtcggccgg
    10201 atgcatggca gcagcaaacg tgtccttcat ccaattgttc gtcgactgga agatgagttt
    10261 gtcgccgctg aacgaaaaat acgcctcggc attttgccgc ccaaacgtga gttgcttaat
    10321 attggtgaga tgccgctcgg tagaagccgt cggtgtcgca gtctctgccg cctttgaggc
    10381 ggcggccgcg gggttcgtca tggaaagaga aagcatcaac cccagcatgc ccgccatcac
    10441 ctgtttagcg tgtttcaatt cgcacctcta cagtcatcct atcctcccaa tctccccgtg
    10501 ctaccacgga cccttcgtga aacaccacat aactctgcca tccgtaaaaa aacaagagcc
    10561 gagccacacg agtcagtgcc ggcggcgtca ccccgtagag gatggtcacg acgctgccag
    10621 gctgatcgtc tcgccggcag gataccaaca gggccatcgt ggggccttcg taagactttc
    10681 cttccaccga aaatcctttt tcggtgaaat gaacatggtc gccgcaatat cgcagcatgt
    10741 ccgaggcggc cgcattttcc cccggcccgc ccagtaccag caccgacccc tccggcgtag
    10801 gcttcgtctt gtcttccggt tgtttcacgg tggtgcgaga cggctcgggc ttcgaggctt
    10861 cctgtgcggc gatacgttga acgatgtcgg taaagggacc cggttgttgt aaggaggctg
    10921 gggccaccac cactgtgcga tgggcgtctg tgacatacac attcaacatg ggcgccaacg
    10981 cattccgcgc cacgcggcga aacacatgga gatctggatc cagccgaacc gctaacgggc
    11041 gttcgggcag cggtatgatc gcattctgtt gggcgtcagt gagatgaata cgcgttcggt
    11101 gggtacgtcc tccatccagt acgaattcca gatcgatggc taggcgatac ggggtaccat
    11161 gttgggcgat gtgcactatc gcctccaccc tgttcatgtc cccggcagtt ccggtggact
    11221 gctggacccg ggacccagtc acctcaatgt ccggcacgcc agcgcgctcg acccactgag
    11281 cgaaaaacca tcgaaggtcg cgccccgccg cctcctggaa cactcgctcc agatcacgcc
    11341 actcggccgc cattccgaga tatcgttccg gtatctgttt caaagccgtc caaaatactg
    11401 cctcaccgat ttcctgcctc aacgcatgaa acaccatcgc ggcttgttga tagccgatgg
    11461 cattgtcttt ttcatcgtgc ttctgggcaa actgtatgac gggatatgcc tctccggggc
    11521 gcacatagac ggcatatccc atgagcatca accgtcgttg ttcgcgagct tgtgcctcgt
    11581 cgcccatgag ctcgtgataa tagtaattcg agagataggt cgtgagccct tccacccagt
    11641 tgccgcgatc gatccgattg aacacgccgt tcccgatcca cgaatgcaca atctcatggc
    11701 caagcgcgta gggctgggtg tagtgccgtt tgatcacgcc gctgcccagc agagtaaagg
    11761 agggcatgcc cagtccgctc gaaaagaaat tttcgacgac ggcaaattga gaaaacggat
    11821 agggaccgag cagagggatg tacgcatcga gataccgcgc cgacgcgtcc aaatactcat
    11881 cggctaagga ggcttcatcc ggaaagagat aggtggccag ccgaatagat tgccccgact
    11941 ttgccgtcca ggtgcgggat ttcaccacga atcggttggc gaccaccgtc aaggcttcgc
    12001 tcttcgtggg aactatccag tggccctggt cggactgagg agctccttgc gtgacagccg
    12061 tccatccgtc aggcagggca atggtgacat cataggtggc gagcgaaccc tccacatcgg
    12121 gataccattg gctttcgctg ctgagataga cgccttcggg tccgatgtgc ccggacgttt
    12181 cactcggcgt gacaaaacga aggtgccgcg ggtcacgtgg cggatcgtcg accacacctc
    12241 gataggaaaa cttcagggct accgctccgt agccccgatc agtggctggc tgcttcacaa
    12301 ttacgcgctg cggctcggat gggcctgctg aaccatccct ctcgaaagca agctcaggag
    12361 ccgtttcgca ggcggcctca cagtgcgtga catcctgaat gcgctcgact cgaagcgtag
    12421 gggcaaggga gaacgacagc gcctgtccgg aagctcttcc cgagaggatc aggcgatccg
    12481 tcgcaatgag ctgatgggag tcgggcagca gctggagcgt cagctgatga tgtgtgatag
    12541 tcggtaggga ggaaacaggc tcagcaactg caagcgcagg cacgagacaa ccggcaagca
    12601 gactgaaacg gatgaggcga tgcaaacaat ccacccttca ccggcacccc gaggttgacg
    12661 gtgaagatta gcattcccga cagaccatca caagctggat tgccatcagg gcgcgctcca
    12721 aatcaagtgg tttatccagc acttcttgcg gccccaacgc ccaggcgtcc tccagcaatg
    12781 gatcattttg atgtccgctc aaaatgatgg tccgcccggc gtactcgcgt gtagccagca
    12841 ctttcagcaa ctccacgccg ttcatctccg gcatgacaag atcaaggatc aacaaatccg
    12901 gcgcggactc ttcaatcatc cgcaaggctt cgcggccatc cttagcccca cgcacacgat
    12961 agccgcgcag actgaggaag cgcatcagca gatcacgtgc catcacatcg tcatcgacca
    13021 cgagaatctg ttcctcggaa ggctgatcga tctcactcgc ggttacatcc tgtgtgcccg
    13081 acgcttcacg tctcgcctgt tgggcgatcc gatgcaccgc tcccaccagc acatccagcg
    13141 acaatccctt tcgaaagaaa tctgtgacgc gtaattttcg tgcgagattt tccagctcct
    13201 cggtagcacc ccctcccaga ataatcactc cggcacgcgg gtcatacgtc cgaatctctt
    13261 tgagcaccgc cagcccatcc atttccggca tgcgcaggtc taacaacgtc acgaccggcc
    13321 gaagctgccg aaagatcgct acgccctcac gcccgcttgt cgccgtgctg acccgatatc
    13381 catggcgcga caacgctacc tggaggagat cacagtgcaa ccggtcatcg tcaatgacta
    13441 ataatgtggt catagtcgat tctcacccat atgcgacagg ccttaccctc agccttttcc
    13501 ggccgaagtc tcacgaaaca gcctagcatg agcttcgtag gatgagaaga aattgacgga
    13561 aactgtagga agggcagcag aacggggccg gttcagctat tggtcaaaac aatgcgtcga
    13621 caaatgcctt ggcgttgaaa tcctgaaggt cctcgacgga ttcccccacg ccgatcaatc
    13681 ggaccgggat tttgagggta tccgcaattg ccaccacgat ccccccgcgg gctgtgccat
    13741 cgagcttggt caacgcaatg ccggtcaccc cgaccgcttc gtgaaactgt cgggcttgcg
    13801 caatggcatt ttgtcccacc gtcgcatcca agaccagcaa cacctcatgg ggtgcacccg
    13861 gacactcctg cgccacaatc cgtttgattt tgcgcagttc gtccatcaag ttggttttgg
    13921 tgtggagtcg gcctgccgtg tcgatgagga ccacatcgga tcctcgtgct ttggcggccg
    13981 taatcccgtc gtaggccacg gctgcggggt cggcacccgg tcgatgccgg atgatgtcga
    14041 cctgggtacg gtccgcccac acttgcaatt gatcgatcgc cgccgcccgg aatgtatcac
    14101 cagcgaccag cagtgcggtg tttccttgtt gccgaaaccg ttgggtcaat ttggcgacgg
    14161 tggtggtctt gccgacaccg ttcactccca cggcgagaat gacgaaggga cgcggccctt
    14221 gcgcgatgag ttggtcgatc gacgcccctt gcgtcggcaa gaggatgtcc aagacggatt
    14281 ggcgcagcag ctcccgcacc ttctcggcag ccgagaaatt ccctccgcgc atctgagcac
    14341 gcaggttctc catgacacgt tctacgaccg gcatccccaa atccgcgctg atgagggcga
    14401 cctccagctc ctctaacagg gccggatcag ccgcgcgtcc cagaaggcga tccagttgcc
    14461 cggtcaccgc attacgtgtt tttgagaggc cctcgctcag tttctgaaac caacccaccg
    14521 ctcacctcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acgctttgat
    14641 gatctcttgg acgagccgat gccgcacgac atcccgttca tcgaaataca caaactcaat
    14701 ccccgcaata tcctgaagga tgttccgcac ttcaatcagc cccgagacgc ggtcggatgg
    14761 caaatcgatc tgtgtgatat cgcctgttac gacggctttg gaatggaagc ctaaccgggt
    14821 gaggaacatt ttcatttgct cagccgtcgc gttctgggct tcgtcgagaa tcacgaacga
    14881 gtcgttgagg gtgcggcctc gcatgaacgc gagaggagcg atttcaatat ctccccgctc
    14941 aatgagacga ttggctcgtt ccatgtccat catatcgaac agcgcatcgt acagagggcg
    15001 aagatacgga ttcaccttcg cgtacatgtc ccccggcaaa taaccgagtt tttcgcccgc
    15061 ttcgactgcc ggccgcgcga ggataatgcg gctgacatcc ttcttcatca gcgcactcac
    15121 cgccatagcc atcgccaaat aggtcttacc tgtgccggca ggaccaatcc cgatgacgat
    15181 gtcatgcttt tcgatcgcgt cgaggtagta tttctgggtc ggagtcttgg gtacgatgaa
    15241 gcgcttccga gtgacgatgg gggacgcgct gaagagcagt tccttgaggg aggcgtcgtg
    15301 attgtggcgg agggcggtca atgcgtgagt gacgtcatct gacctgagtt cgtagccttc
    15361 gttggtgagc gatgcgagtt cattgagtac tcgctcggcc tgcttcacgg catcggcagc
    15421 accctctacc gtgacttctt cgccccgtgc ggaacaccgc accccgaact cttcctcaat
    15481 caatttgagg tggcgatcgt ggtgtccaaa taggaccgca gtgttcgtgc cttcacgtag
    15541 tttgatcttg cgcacgcctg tgtcgaagac tcctttcgcc aagctgatgt ctagattcta
    15601 acaaaccgcg aaagggagga acaaggtcgg aacagggggg gtggggaaaa attagcggaa
    15661 ctgaggtctc gagaagccgg ctacccttga ggcggcaacg agccctgcga ttgcgccgtg
    15721 gagggaatcg agagttcaaa ctcctgccag gcaattccgc cctgcccgtc ttccgccatg
    15781 atcttcactc gatgagttcc cgccactccc ggaatgactt tccagctgac ctggcctgtt
    15841 gccctgtcaa tggtcattcc cggggggcct gtctccaaga catagctcac cgtatcgcca
    15901 tccacatcct tggcctggac cacatactcg tattgttctc gattcgtgag cgccgccggg
    15961 ttggaaacaa tcaacggcgg tgaattcccc aacgcgctcg gggccgaccg gaccggcgca
    16021 gctgctgcat cctgatcccg cgcaatcact tcaacgacca ccacatcttt tcgcccaaat
    16081 cctgtggtgt cgaggatgct ttcctcacct tctttgacct gtttgtcgtt gcgccaccac
    16141 cggtagttgt agtggatttc atctcgatca ggatcgacgg catcgacctt ggcaagaacc
    16201 cggctgttat gttcagcatc tgcctcaacg gtgacatgtg acacgagagg gggagtgttc
    16261 accacagaaa ccggctccgt gcgataggga gcgctttcca gctcaccatc gaaggctaca
    16321 acctccagcg aaacctggtc tcctcgcttc acatggaccg gatccagttc aaggccggtt
    16381 gcccccaaaa caggagcact gttgacgatc cactgaaatc gcttggtagg ctcggtacca
    16441 tcagggtcat ctgccatcac atgtgccgtg ataggtcctg cgagtatgag tgggtttggc
    16501 acaatcgtca caaggcgaac aacgggagcg tggttattct ttgtcgcacc actgaccact
    16561 gatccgtctt gcggagagga cgagcacccc cctacaacgg cgacgaccga agcagccaac
    16621 agccatcgag cggatgaaat gacggtgtgg ggacagagtc tttccccaca cctttcaacc
    16681 aggctccaga caccagtctt cattgattgg tccatgagaa aaatctactc cgcgggtcaa
    16741 tcgcgacagg aacgctgatg ttcgatccaa acatgcctcc acctgtcgtg caggtcgcac
    16801 agtctccgaa atttccctgg ctcatgttga tcagcaaccc gaacgggctt ccgccgtctc
    16861 tgccggagcc catatgcacc acgacaccga atgctaaccc ttcgcctagt gatccaaatt
    16921 tgttgaccgt ctgattcgcg cctgctcccg tcgtacccaa aatcggctcc tgataggcgc
    16981 ttcctgtttt gtagaacagg gcccagagcc ggctggtgcc agacgacacg cagatatcat
    17041 tggtcggaat aaaggtgggg aaaaatacga cccctccaaa caccgtcggg ctactgacgg
    17101 cacgttctcc gataccatac ggaaccggct tcgtgggtgg attggcaggc tcgacgagct
    17161 tggtcaccca tccatccttg ctttggacca acccaatcag tcctgtgaac gtggtcgcgc
    17221 cggtcacacc ggtcacctga tttgtaccgg tcgccagtcc gcaatcgcca acgcccacca
    17281 cacacacagt ggcattggac acatccacga gatcgctgtc catgcaggcg gtgataccgc
    17341 ttgattgatt gcacccagca ttgagtactg aatccttcat gcctacgaag tactgggtgg
    17401 agatgtcggt cttatcagac ttgctgtaat aacggccgct ccccacaaac acccagacat
    17461 tagccgcgtc atctgtcgtg acggaagggg cgcttgcaac cgggcccatc tccgctccgg
    17521 acgcaaaggt atccaacatt tccgtcggga cgcgcgcgct accggaggaa atcccccatt
    17581 gcgaagggct cgtgaccgta ccgaattttg aggttgcccc gcctgccccc atggtcagcc
    17641 gatagatctt tcccctccaa gggagagccc catcattgat gacgcggccg aagtagacga
    17701 catcgtgacg atagtcgagg tcacgatcga acgccgtcat atctccgatg tacgaatccc
    17761 atgacccgat ggaatatgag gtcaccaaac tgttactgct tcccggtcct gtcgcgagat
    17821 cgatggcaaa cactttcgcg ccctgcgcca cgctggcgtc gtacccggtc gggccggacc
    17881 cgacgaccat gtaccacttc gcgttcgtat tgctggcttt ggcatcggcg gccggattca
    17941 cacgcacgac cgccgggacg ctggtcgaca atcccaaatc cgagctgctg aatgaccaca
    18001 gcaatctcgg gctatcggga ttggtgacat ccatcgcgaa ataagcgcta tagaaattgt
    18061 agttggttcc gccaatgttc accgtcatcg gcggtgcacc agttcccgcc acacaggcac
    18121 cgcaactgcc tcccattctg aagccgccga tcagaatagt gccccatcca ttgggatgat
    18181 cggtatccgc ggtgaagatc cgcacatcgg cgaccttgag aggcaagtca acatagtatg
    18241 catgttgata atcggcacgg ctcagaaact cgagctgagg gaggagttca taggggacaa
    18301 atccccataa ctcatcccca agcggagctc cgctggaatt gtcggcggga gccgtcgtaa
    18361 accaaccatg ttcggttgtg ctggcgggcg cagacgtgct cgtatcgtca ccgggatggt
    18421 agtaaccggc gttgaacgca tgcagcatgc cgtcatttcc gcccacgtag gcaacctgtc
    18481 tgcggttgta ccacttctgc aggaacgcgg tgtaactctg gtctccgtag atcgcatcgt
    18541 gacggtctcg aggacccgcc acgacagtcg gcgtcgagtg gatgacgtct cccagcttcc
    18601 acacttttaa cgttccgctt cccgaaggaa cctggagacg gcgatctctg gtcgtcgctt
    18661 gatcagtgca tgttgtggca tcacatccac ggataaagtt aataattttg gttccctctg
    18721 cactggacgt ggcacgcagg tatggacgaa gcgttgtttc gttcgcggtg gagaacgcga
    18781 tctgctcacc ggaatccacc actccgtcat ggtcggaatc gacccaagtg agaatggtac
    18841 gggtggatgc atcttttaac gccaattgct tcccggcttc ccagatcggc aatatgtcgc
    18901 tcaatagttt gccgcaggga ttgcaatccg ccagcgtgac gacgccgtcg ctgttctgat
    18961 cgttcggcaa tccatcgcca tccgaatcga catatttatc gaccttcacc gagttagaga
    19021 gtgagtcgaa gcgggtcttg atgatcttgt cgaccttgta gtcaaggcga ccgtcctggt
    19081 tcgtgtcctc tcgcgtgttg ccgaacgtat cgataaacaa tgcctgggta tatcccgtcc
    19141 atttcacgtc actcaacgtc gagggctcga tcgtgctggg atagaaatag gattgataca
    19201 gcgcgccttc tccagttgaa gcagtggcca ataccgacac cgaagaaccc gacgcgctcc
    19261 gccggagaat acttgcaatg gtggccaaca acttgtcttg gagatcgtcc acgttcgagg
    19321 attcgaaata cgcatccgga atgccgtcgg gtgttgacgc tccggtcaaa ttgttctctt
    19381 tgtcccattc ggaagtctgg gggatcccat ctccgttcgc atcttcaaat ccccccaagc
    19441 gggcggtttg ggcgagaatt cctcgaccat tgatattgcc aaatgcaaaa aatgaataca
    19501 cggtgagatt ctgcgtgccg ggtatgcaat gcccagtgac agataaccct gcgatcgtgc
    19561 cgtcgctcgt tccactgcaa gggcgcaaat cactggtgtg cgcccaatag gccacatcgt
    19621 ctaagtaatg gcttccgctg tcggcgtaat cagtcttgtg ttcagccaac agatcactga
    19681 agggggtcgc cgcattagta ttacaagtgc cgttgatagg ccgaggagga gcagcgctat
    19741 cgctgccgtt acaatgctgc ccatgatggg catgggcata gtcctgaatc gccgtcggta
    19801 cattcacgtc ttgcgtcggc tcaccgtctg taaaaataat gacgaacgtc cggcagcaat
    19861 tgacgacctg agaagtcgac gcccaaggcg gcgtgtggtt ttggccaaaa aagtaggggt
    19921 cgcggccaca ggcgttcgtg atgtatccag ccgggcaggt ttcggtacca accaatgccg
    19981 tgatttcgtc cgtgcctatg gaaccagcac cggtggtggc aaaattgact ccgtttgaaa
    20041 tgcctcctga aaacgcgatc ggatacacgt agcctgtgac aaacgttgat tgaatctgcg
    20101 caacataccg tgccgtctca tagagtgatt cgctcagtgg cgtccaggtg gagggatacg
    20161 actcctgcac cgcgtccacc atagccgcca tgttcgtatt gaacgtctct actcctgtac
    20221 cattccagtc aatggattga cgagaacccg cgccgactaa catgcgcgca ccttcactac
    20281 tggacttgaa ttcgacgagg ccgaagcgag ccttgctccc gatctgctga atgacccccg
    20341 tgggttcagt cgaataccca accttcaatt catacttacg aacgctgtat gagtctccgc
    20401 aattactgtt gaaactggta tcattgtcca cacaaaaata cggcacagcc ggagttccgc
    20461 tcgcggagat accaatcgag atcgtccctg gattgctacc ccgatcggct aacggaatgc
    20521 gccccacata cgtgttggct ccagtgccac cgctgtaatt cacgtcggct cgttcatcgt
    20581 tgaccccggt ggcctgggca cgcacagttt tcaacgcagg cgttccactg gtcgggcagg
    20641 tgccatccgt cgctctattt acggcacagt ctcctccgga catcgcccgt ttcaccgcat
    20701 cgaaccggcg cagtgtcgcc caattcaaaa aattgccatc ccattccgtc gtcgaacaa
//
LOCUS       DAOQEW010000051        20746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_637_length_20746_cov_9.053695, whole genome shotgun sequence.
ACCESSION   DAOQEW010000051 DAOQEW010000000
VERSION     DAOQEW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_637_length_20746_cov_9.053695"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..533)
                     /locus_tag="PLS35_07620"
     CDS             complement(99..533)
                     /locus_tag="PLS35_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18565.1"
                     /translation="MTWWTPQLNALLGGLVLAGGIFVMWDQAGLVWTLLVAVAATAFL
                     LWRGRSIGTVWAWTTLGLGIESMTWPIVTMVRIRVEGIQPTEEQMGTILNAVLFGLFT
                     SVFWVTFALGLFKRLRAQHAPPVPVQQGQQATPRGKKKRSRN"
     gene            complement(646..1017)
                     /locus_tag="PLS35_07625"
     CDS             complement(646..1017)
                     /locus_tag="PLS35_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18566.1"
                     /translation="MPSVLIVDDEEAIRRLIRSTLEQAGYRVQEAADGKEGLSHYRQA
                     PADLVIMDILMPDQDGLESILTLRREFPNAKIMAITGGSDMIGILNFLDVARMLGARR
                     TLQKPFEMQQLLDAVQAEIAG"
     gene            complement(1124..2734)
                     /locus_tag="PLS35_07630"
     CDS             complement(1124..2734)
                     /locus_tag="PLS35_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18567.1"
                     /translation="MSAQDLAGAFPSSGDFRSIEAVLRTLSSQASIGIFLSDPEGHTL
                     YLNERLRRMAGLPTAPASPECWLTALAPEDHDVIVTEWLTATEQARSFSREFRFCRPD
                     GSLRWVMAEAFPLRTEGGPPTGYVGMIRDITPRHLALEALHAAEDRYRSLAALSPHPV
                     LVYADGTILFMNDAGMRLFSVPPAASVEGHSLSDVFPKAFLQDLPVANPIHGTEGATP
                     VERQFVRPDGTRLDLEVVASQVTVDGHPAIQVLATDITAQKDAAAQVRRAHKMAAVAT
                     LAGGIAHEFNNCLTAIMGFSDLALPLLVPDSRVYGHVQQVVLASKRARDLVTQMLMFG
                     RQAEGIKQPVSLDILLKETLRLLKGTLPDNITLREWIPGATHPIYADPTQIHQVCVKL
                     LAHSELAMKPDGGILEVRLDNVDLTQSTNAEDLPLRPGHYVCLTVSDTGEEVSAQDHR
                     HKVGPLFAHLPDGTQAGTELAGVQRVVSEHGGTLHSTSTVGQGTTIEVYLPAILPPRA
                     IGAPELPRADASDITGQKEFLAERDKER"
     gene            complement(3138..3221)
                     /locus_tag="PLS35_07635"
     tRNA            complement(3138..3221)
                     /locus_tag="PLS35_07635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3186..3188),aa:Leu,seq:gag)
     gene            3449..4144
                     /locus_tag="PLS35_07640"
     CDS             3449..4144
                     /locus_tag="PLS35_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNI18568.1"
                     /translation="MTPLSAPHISCTGRQTDIAALFDVDNTLLPGQASEVRFFRFLWK
                     RGLVGWREVRDSIHWVLRCAPPVSLHPLRERKLYLAGKGAAEVEALAEEFCRTELCPR
                     LSAQGLSRMDEHRRAGHHIVLVTGSLDFLIAPLAALLEVPTLLAARLEQQEQQFTGHV
                     CAPLPYGPGKRELIARLTRESGIDLAQSFAYGDSPGDVELLEMVGHPLVVNPIRGMDR
                     IAQRNGWPMTTWK"
     gene            4195..4833
                     /gene="queE"
                     /locus_tag="PLS35_07645"
     CDS             4195..4833
                     /gene="queE"
                     /locus_tag="PLS35_07645"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNI18569.1"
                     /translation="MLRVTEIFHSIQGESTHAGRRCVFIRLTGCPLRCTWCDTAYAFY
                     GGQDLTEEDVVAQVRAFGCNLVEVTGGEPLSQAACLPLLRRLCDEGFEVLLETSGAVD
                     TAGVDERVHVVLDVKCPGSGMVDRMYWPNVDRLTRRDEVKFVIKDRTDYEWARKVVRS
                     RDLEARCTVLMSPVFGEVDARQLAEWVLADRLPVRFQLQMHKQIWAPDMRGV"
     gene            4898..6418
                     /locus_tag="PLS35_07650"
     CDS             4898..6418
                     /locus_tag="PLS35_07650"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNI18570.1"
                     /translation="MKKIQVQAQVGRAEQEGAEVLVLLCCEGASDLSPEAAAINTQLG
                     GQLATLIQRGEFEGKLGEGLLVHTQGKAKAKRLLLAGLGKEKDLRLDAFRQALGAAVK
                     RVRQAKVSSFGVVLPGAVLEEIPVQDVAQAMAEGAILGNYQFTAYRSSNGSKPVEVER
                     LTIYVAQTSLLAQVTEGIRRGVATAEATVLVRDLCNHPANIMTPGRIVQEAKAVAKEP
                     GVKLKVLEQKDMEQLGMGALLGVAKGSHEPPKFIILEYKGAKAKKGDPPVVMVGKTIT
                     FDTGGISLKPAENMEHMKADMTGGAEVLATMRAAARLKLPVHLVSILPVAENMPGGRA
                     MRPGDIVKTLSGKTVEVQNTDAEGRLILSDALAYATRYKPAVLIDIATLTGACVVALG
                     QFAIGMFGNNDQLKEHVRNAGMRAGERVWEMPLWEEYFEQLRSDVADMRNIGGRGGGM
                     ITAALFLSKFVGDCPWIHLDIASTDWSERERAYLPKGPTGIGTRLLIQFLLDRTLP"
     gene            6493..7644
                     /gene="nagZ"
                     /locus_tag="PLS35_07655"
     CDS             6493..7644
                     /gene="nagZ"
                     /locus_tag="PLS35_07655"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNI18571.1"
                     /translation="MTLREHIGQLFMMGFTGTTVSKDLASFLTAYKPGGVIFFRRNLE
                     SVQQIVDLTNGLQKLSPASPLLIAIDQEGGRVSRLPAEFTIFPPCGQLGQCHSSELAY
                     SAAATIAKELRAVGINMNMAPVLDVNSNPDNPVIGDRAFGAEPDVVADMGLATIGGLQ
                     DNMVVACGKHFPGHGDTATDSHKELPVVDAGIQRLRETEFPPFQQAIRQGVASLMTAH
                     VLYRALDPDAPATLSPAVIQRLLREEFRYDGVVFTDDLEMHAIIDHDGIGEAAVRSFV
                     AGCDVLLICKDQDRVMTAMQAMERAVQNGRITQERLEQSLARVARLKARYLIPYKPVT
                     ISDARLVVGCRSHKVLLDSWHKAYARLPSSKVSEHDEPAAALDSPVTHV"
     gene            7795..8184
                     /locus_tag="PLS35_07660"
     CDS             7795..8184
                     /locus_tag="PLS35_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18572.1"
                     /translation="MGIKKGDILDERKRFPDSCGLVVKCTGGGEGFQKIFCCGHELTE
                     EDLVSEIVSSRGRKRGTLLPGAMLEERRQFPDSCGLRLMVMDGGAGLQGIDCCGHLIT
                     AGAVHGLRFGQPLDEGAPMPTGPAGQA"
     gene            8184..8705
                     /locus_tag="PLS35_07665"
     CDS             8184..8705
                     /locus_tag="PLS35_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNI18573.1"
                     /translation="MRAPSMPGADEKFHRPSMRWWLGFMDQLDRLGYVAAGFSLLALC
                     LIIFVHAWYIFLARPVQLALLPAALKLLNDLLLVIILLELFRTVVRFLQTEVLELEPY
                     LSVGIIACTRRLLTASAELSYQLEAVAKESRHELFQQYLLDVGLNVLVIIILIGGLYL
                     LRSRPSTERPAVS"
     gene            complement(9118..9285)
                     /locus_tag="PLS35_07670"
     CDS             complement(9118..9285)
                     /locus_tag="PLS35_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18574.1"
                     /translation="MTPLHEHFDDGETKTTLRLARLGRPWVRLRQGPQQGRPVWQQPL
                     PVQLGQVRQLK"
     gene            complement(9282..10727)
                     /locus_tag="PLS35_07675"
     CDS             complement(9282..10727)
                     /locus_tag="PLS35_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNI18575.1"
                     /translation="MGNPLFRTKSIERILADSDAPEHRLKRSLTAWDLTGLGIGAIIG
                     TGIFVLIGTAIVGDAHRPGAGPGIVLSFILSGITCALAALCYAEFAAMIPVAGSAYTY
                     SYATLGEFLAWLTGWNLILEYGVACVAVAIGWSGYFNNILKLCGLELPYWATHPPGAD
                     GGIANIPAAIIVLLVTGILIVGVKESARATCGIVLIKLAVIVFFIAVGTSSVDTTNWT
                     PFMPFGFAGVGAAAAIVFFAYIGFDAVSTTAEEAKNPQRDLPIGIFASLGICTLLYIA
                     VAAVLTGLVPFSRIDIHAPVAEGLRVAGFKWGAAIVAVGAVAGITSVLVVMMLGQIRV
                     FFAMSRDGLLGPWLSGVHPKFRTPHHATYLTGIAVAIMAALIPIGEAADMTNIGTLFA
                     FVLVCIGIIVLRYTNPNHPRPFRMPFMPVIPMLGVLACLGLMYFLPWMTWIRFFVWTV
                     IGIVVYATYGIRRSKLATSPALEHTSHARRA"
     gene            complement(10791..11567)
                     /locus_tag="PLS35_07680"
     CDS             complement(10791..11567)
                     /locus_tag="PLS35_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNI18576.1"
                     /translation="MKPVIGVTPDFNAGDRQEWGGKEPTYFLRARYVRAIEELGGVPL
                     ILPLVADRAARRRLLRTIDGLLLTGSGPDLDPALYGERKQYSFPIVAQRRSSFELDLV
                     HLATRNHLPTLAICGGMQTMNVAFGGTLYQDLPAQVPDVLQHRQTTPAIQLAHSVSIT
                     PGTLLERIMKRPRMKVNSSHHQSVKVVGRALIASATAPDGIVEAIEHPGHPFFLGLQW
                     HPEFLFERHRLHRQLFEAFLRAASRRPALRPLSPQRGTTT"
     gene            complement(11569..12045)
                     /locus_tag="PLS35_07685"
     CDS             complement(11569..12045)
                     /locus_tag="PLS35_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18577.1"
                     /translation="MNVSVNLSSKRRRSLWSNALVLGLVAICSLAILIVPVAAQESGS
                     ASNVPVSSNDASPSGVGIQAGSALATIIYFPLKLAFAIGGGVVGGLAYGFSGGSEQTA
                     KNIWIPSMYGTYIITPDHLKGDKPIRFLGVSSSEPTREAGDYTPQEPLPLPLEPIR"
     gene            complement(12202..12663)
                     /locus_tag="PLS35_07690"
     CDS             complement(12202..12663)
                     /locus_tag="PLS35_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18578.1"
                     /translation="MSIPPAATEPSQYRVTFFFGPEPVEHRPDLLRCVFNVKKRSWKG
                     GIQVSLDIAMKHVEEARRAIGFSTWVEHQLHKICQEEQAELTRRADELFIQSVCTCAL
                     NLALHGGIEQHNQVIDAARFTDELRQIMTEQPEQVMERIRLELDLEESPDR"
     gene            12755..13972
                     /locus_tag="PLS35_07695"
     CDS             12755..13972
                     /locus_tag="PLS35_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNI18579.1"
                     /translation="MKTSADFVAQVDRIQEAIRAHPGIDGWLFYDFRHLDPIAYRVLL
                     LDPSMHVTRRWYYWVPAVGMPVKLQHRIEPHVLEALPGEVRFYVSWREQQTALRALLQ
                     SAKRIAMQYSPMNAIPYLSRVDAGTLDLLRSWGLEIVTSADLVQQFEAVWDEAQLASH
                     QAAATGLRAIVDEAFGFIGTSLAAGSSLNEYGLQQYILSRMHARGLVTSSPPIAAVNA
                     HSADPHYGPSAEGSAPVRQGDLVLIDLWAKQPEARAVYADITWTGFVGKVVPARQQDV
                     FQIVRQARDAAVTFVQARVREGTFPYGWEVDDVCRQVIQQAGYGEHFVHRTGHSIGEE
                     VHGNGANIDNLETQDARRLLPGTCFSIEPGIYLPGDFGIRSELDVYLSTNDAVVFGQP
                     VQTELVAITLPTT"
     gene            14107..14961
                     /gene="tatA"
                     /locus_tag="PLS35_07700"
     CDS             14107..14961
                     /gene="tatA"
                     /locus_tag="PLS35_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNI18580.1"
                     /translation="MFGTMGFSELIIILVIVLIIFGAGKLPQIGEGVGKALRGFKKEV
                     NDIPAADATPEQGEAATPSSQAQIPPVAEVAQTAQPAPVAQAAPSLKPTAPYTPGPEL
                     TPGTTAALMAAAAPQGPQTAQASKPRVATVTPTQAAPGSVVAQGHQPPTMEDRMAAPA
                     PVMRGQYPPLPASAQSKPVAKRPSAIVNKDAVARVQAAQAAIRAKAAQPQPAAFSPQD
                     MQGLGEGLGDAVRTFRQAVADVRSSVDPQMRTIRAEMDSAQKELEQSIEAAKQAPVLD
                     EDTPAKPL"
     gene            15276..15962
                     /locus_tag="PLS35_07705"
     CDS             15276..15962
                     /locus_tag="PLS35_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18581.1"
                     /translation="MRLNELLADPDPEVRRTAAEGLGKIGHPSASPGLIAALADRDPH
                     VRAAAALALGRVGDAKSGAALVEHLSDSSESVRTASALALGEIGTSVNHEEKILRALR
                     QPEVSTRLAASRALLSLETVSFSTDLLSALDDPNAQVRQGVVAVLSETGDRRPLPSLM
                     RLLQTDEDAGVRAEAAFRLGKVGDAGVVPALSVVAEKDSNPTVRQWAHWAVRQITTSP
                     GFGSGNRQGQ"
     gene            complement(15916..16434)
                     /locus_tag="PLS35_07710"
     CDS             complement(15916..16434)
                     /locus_tag="PLS35_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18582.1"
                     /translation="MKFPVTATREHHGKTLGIDCEQETVSLFDHKGQQLSILSWGTLI
                     EHMLSVDEDARFAHSRSHPRAPLAIKVHCTTEDGKHFDSLTGGIGGGGLFIESSTPLA
                     PGTELTVEFALPDRPNQKFATRAKVAWTRSKPERYLLFPGMGIQFTDIDPVAQTRLID
                     LVDSLNRSRAMS"
     gene            complement(16511..17029)
                     /locus_tag="PLS35_07715"
     CDS             complement(16511..17029)
                     /locus_tag="PLS35_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18583.1"
                     /translation="MKFPVVSSTRHKGKTIEIDSSKESITLLGINGEPMGSLAWDFLV
                     DQILTYRKPQQSREARSEPRISLSIRVKYHTPDGMQFESRAGGIGGGGLFIESLAPLA
                     VGTKLAMEFTLPEDPSEWMSAKGVIAWVCPKADQYTFSPGMGVRFTDIASETRSRVLN
                     LVHSVKGIPQSE"
     gene            complement(17240..18496)
                     /gene="clpX"
                     /locus_tag="PLS35_07720"
     CDS             complement(17240..18496)
                     /gene="clpX"
                     /locus_tag="PLS35_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNI18584.1"
                     /translation="MAKQEKIDRHLRCSFCGKSRDEVRKLIAGPTVYICDECVNLCND
                     IIAEDWEEAKEEISSKLKKPAEIKHHLDQYVVGQDRAKRILSVAVHNHYKRISAKDKD
                     VDDVELQKGNILVVGPTGTGKTLLAQTLAKFLDVPFTLADATTLTEAGYVGEDVENII
                     LKLLQAADYDVERAERGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEGTVA
                     NVPPQGGRKHPHQEFIQVNTSNILFICGGAFVGLEHIIEQRLNRKSMGFGAEVRGRND
                     IRLGELLPHVQPEDLLKYGLIPEFIGRLPVVATLDELDEQALIRILTEPRNALTKQYE
                     KLLSFEKVKLRFTEGALSAVARKAFTQKTGARGLRAILEEAMLDVMYDAPSQKQIKEV
                     VITEDAISGKHAPIRIFEQDKDVKSA"
     gene            complement(18523..19158)
                     /gene="clpP"
                     /locus_tag="PLS35_07725"
     CDS             complement(18523..19158)
                     /gene="clpP"
                     /locus_tag="PLS35_07725"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNI18585.1"
                     /translation="MLVPIVVEQTNRGERAYDIYSRLLKDRIIFLGAPIDDVFANLII
                     AQLLFLEAEDPEKDINLYINSPGGSVTAGLGIYDTMQYVKPSINTICLGQAASMGAFL
                     LTAGTKGKRYALPNARVMIHQPMGGFQGQATEIDIHAREILKIRERLNEIMAKHTGQP
                     LDKISQDTERDYFMSSEEAKRYGLIDEVITRPLKSLKPVGSTDSGKDGGKS"
     gene            complement(19283..20608)
                     /gene="tig"
                     /locus_tag="PLS35_07730"
     CDS             complement(19283..20608)
                     /gene="tig"
                     /locus_tag="PLS35_07730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNI18586.1"
                     /translation="MKMEMTELGPMKRALKIEVPADEVNLRFVQAYSELNRQVRIPGF
                     RPGKAPLQLLEKRYAKTIEEDVIRSLVPDYYDRAIRQAGIVPVLVEIPPLERVKVKKD
                     SPFSFTATVEIKPTIELRDYKAPNPISLKQDQRTVTDEQVQKAFEVLREQMAQLHPAP
                     AGTTLADGDYAVVDIDGTVDGAALDGTTKTGHLHKMGSQSSVLGIEIEPHLLGKKEGD
                     VITIPQAYPATHPDARVAGKTVNFRCTVKSIKRKQLPALDDEFAKDCGPYQSLQEIRE
                     KLHAEMDRALKKDIEDSYKDTILKRLAETHHFDLPGTLVERELSAMVRQQLQSRQRKT
                     GDAADGSPGASQADEAKQLQEEYRPEAERRVKVGLILEALATKENITVTNEDLSNEIA
                     RLAAEVKLSVEEVTRMIQAGGQDTLNDLRSRILADKALDFVYKHAMIQG"
BASE COUNT         4177 a         5652 c         6287 g         4630 t
ORIGIN      
        1 tagccgtgcg aacgcgtgag gacgtggagc gggaatcacg ccgtggtggc ctccgttatg
       61 atattgacgt cgatccggtc tcgatggcct gaggcgagtc aattccggga gcgtttcttc
      121 ttgcctcttg gtgtggcctg ttgtccctgc tgcaccggaa ccggtggcgc gtgctgtgcc
      181 cgcagccgtt tgaacagacc aagggcaaac gtcacccaaa acaccgaggt gaaaaggccg
      241 aacagcacag cattcagaat cgttcccatc tgttcttccg tcggctgaat cccttccacc
      301 cggatgcgta ccatggtcac aatcggccag gtcatgcttt cgataccaag ccccaatgtt
      361 gtccaggccc atacggtccc gatgctccgc cctcgccaga ggagaaatgc ggttgcggca
      421 acggcgacaa gcagcgtcca gacgagtcct gcctggtccc acatgacaaa gatgccgccg
      481 gcaagcacca ggccgcccag tagtgcgttc agttggggag tccaccaggt catgatcacg
      541 gtctcgttga tgagtacggg gagacgaaag cgggcgtagt gtgcggatgg atcggccgga
      601 agtcaatgga ctgccgtgcc gcgaacatcg agtcggcgtg tttcgttagc cggcaatctc
      661 tgcctgaacg gcgtcgagca gctgttgcat ctcgaatggt ttttggagcg tgcggcgggc
      721 gccgagcatg cgggcgacat ccagaaaatt gaggatgccg atcatgtcgc ttccgccggt
      781 gatggccatg attttggcgt tggggaattc tcgccgcaag gtcaaaatgc tctccaagcc
      841 gtcctggtcc ggcatgagaa tatccatgat caccaaatca gcgggcgctt gacgatagtg
      901 cgacaaacct tctttgccgt cggcggcttc ttggacacga tagcccgcct gctcgagcgt
      961 gctacggata aggcggcgaa tcgcttcttc gtcgtccacg atcaaaaccg atggcataac
     1021 gttcctctca ggagttctcg tgatggctct ctgggggcaa gtggtgcggc cctgcagagg
     1081 tggtaattcg gatctctatg agttgccggc actcatcgcg aatctatcgc tctttgtcgc
     1141 gctcagcaag aaattccttc tgtcctgtga tgtctgatgc atctgcgcga gggagctctg
     1201 gtgcgccgat ggccctgggt ggaagtatcg caggcaagta gacttcgatg gtcgttccct
     1261 ggccgactgt gctggtcgag tgaagtgttc ccccatgctc actcacgacg cgttgaaccc
     1321 ctgcgagttc cgtcccggcc tgggttccat ccggaaggtg ggcgaacaac ggtcccactt
     1381 tatgacgatg gtcttgggca ctcacttcct cgcctgtatc agacactgtg aggcaaacgt
     1441 agtggccagg gcgaagggga aggtcctctg cgttcgtcga ttgggtcagg tcgacattgt
     1501 cgaggcgcac ttcaaggatg ccgccgtccg gtttcatggc taactcggaa tgggcgagga
     1561 gcttcacgca aacttggtga atctgtgtcg ggtcggcata aatcggatgg gtggccccgg
     1621 gaatccattc gcgcagggta atgttatccg gcagcgtccc cttcaaaagc ctcagcgttt
     1681 ctttcaggag gatatcgagc gagaccggct gcttgatgcc ctcggcctgg cggccgaaca
     1741 tgagcatctg ggtcaccaga tctctcgctc gcttcgatgc aaggaccact tgctggacat
     1801 gcccgtagac gcgactgtcg ggcaccagta acggcagggc gaggtcggag aatcccatga
     1861 tggccgttag gcagttgttg aactcatgtg cgatgccgcc ggcaagagtc gcgaccgcgg
     1921 ccattttgtg cgcccggcgc acctgcgctg cggcatcttt ttgtgcggtg atatctgtgg
     1981 ccaagacttg aatggccggg tggccatcaa cggtaacctg gcttgccacc acttccagat
     2041 caaggcgcgt tccatcgggg cgtacgaatt gtcgctcgac aggggtggcg ccttccgtcc
     2101 catgaatggg gtttgccacg ggaagatcct gcaggaatgc cttcgggaaa acgtccgaca
     2161 gggaatgtcc ttcaacagag gccgctggcg gtacactgaa gagccgcatg cctgcgtcat
     2221 tcatgaacag aatcgtcccg tctgcataga ccaggaccgg atgaggcgac agcgcggcca
     2281 agctccggta gcggtcctcg gcggcatgta acgcttcgag tgccaggtgt cgcggggtga
     2341 tatcgcggat catgcccacg tatcccgtgg ggggcccccc ctccgtgcgg agtggaaacg
     2401 cctcggccat cacccaccgg agtgatccgt ctggtctgca aaagcgaaat tcgcgtgaaa
     2461 agctccgggc ctgctcggtg gccgtcaacc attcggtgac aatcacgtcg tggtcttcag
     2521 gagcaagagc cgtgagccag cattcgggtg atgccggtgc ggtaggaagt ccggccatac
     2581 ggcgcagccg ttcattgaga tagagagtat gcccttccgg gtccgaaagg aaaatgccga
     2641 tcgaagcctg gctggataag gtacggagaa cggcctcaat cgaccggaaa tctcccgagg
     2701 aaggaaaggc ccctgccaag tcttgcgcgc tcacacgtca ctccttcgta cgcactgagg
     2761 gaactgatgc ggccagcagt gccgcccacc gtaaagtttg tccggctggt ggtaatggcc
     2821 ctccagaatc atggggagca cgaatacgta tctcagtgcg cttcatccct gcaggattcg
     2881 catcatatca ccaagccgga tcgtgcggtg agtgatgatt gaaattttgc aacgtaattg
     2941 ggccctagtg cagtagggcc gtttatctgc gtgaagggcg gcatcgggtg ccaccagccc
     3001 ataggtattc gcagtgagaa tcagatgtga atcgatgatg tgtgtgctgg aagaaggaga
     3061 ctcgccgagg gttgcttgcc tttccgtccc ccctgcaggt tgcagagact ggaaggccct
     3121 tctgccattg ctgagtttgg tgccgaaggc gggacttgaa cccgcacggc ttgcgccaca
     3181 cgcccctcaa acgtgcgtgt ctgccattcc accacttcgg caaccgaatc gctttccgtc
     3241 aacacatgag gcagggtatg acgagccgaa cgccgatact gaatgggccg ttcttgctac
     3301 gtagtccccg gacctcagag gaagcgcatt atagaagtgg tccaaaatgc ttgtcaatca
     3361 actagttgtc cggtagaatc tgacgcgtgc tgcgtggaca cgtggcatcc ccttcccata
     3421 tacagcgatc agcggaccaa cgttcgagat gacgcctctc tcagccccac atatttcatg
     3481 cacaggtcgg cagacggaca tcgccgcgct ctttgacgtc gacaatacgt tgctccccgg
     3541 acaagccagc gaagtgcgat tttttcgctt tctctggaag cgaggactcg tgggctggcg
     3601 tgaggttcgg gacagcattc actgggtgct gcggtgtgcg cctccggttt cgttgcatcc
     3661 gttgcgggag cgcaagctct atctggccgg aaagggtgcg gctgaggtcg aggcgttggc
     3721 cgaagaattt tgccgtaccg aattatgtcc ccgtctgtcc gcccaggggc tctctcgcat
     3781 ggatgagcac cgtcgagccg ggcatcatat tgtgctggtg accggatcgc tcgatttcct
     3841 gattgctccg ctggccgcat tgctggaggt gccgaccttg ctggccgcca ggctggaaca
     3901 gcaggaacag cagtttacgg ggcatgtctg cgcgcccctc ccgtatggcc ccggcaagcg
     3961 ggaactgatt gcgcgtctga cgcgggaatc aggcattgat ctggcgcagt cctttgccta
     4021 cggcgatagt cccggcgacg tcgagttact cgagatggtc ggtcatccgt tggtcgtgaa
     4081 tcccattcgc gggatggacc gtatcgcgca acgaaacggg tggccgatga ccacatggaa
     4141 ataaagattg ccgcaggccc atcgtcacga agtgatcagc cagatcgatc cacgatgctc
     4201 agagtaacgg aaatatttca cagcattcag ggcgagtcca cgcacgccgg tcggcgctgt
     4261 gtgttcattc gtttgacggg atgccccttg cggtgtacct ggtgtgatac cgcctatgca
     4321 ttctacggcg gtcaggatct gacggaagag gacgttgttg cgcaggtccg ggccttcggg
     4381 tgcaatttgg tggaagtgac cggcggtgag ccgctcagcc aggcggcatg cctgccattg
     4441 ttgaggaggc tctgtgacga ggggttcgag gtgcttctgg aaacgagcgg ggctgtcgat
     4501 acggccggcg tggatgagcg agtccacgtg gtgctcgatg tgaaatgtcc cggaagtggc
     4561 atggtcgatc gcatgtactg gccgaatgtg gatcgactca cacgtcggga tgaagtgaag
     4621 tttgtcatca aagaccgcac ggactatgag tgggcgcgca aggtggtgcg cagccgggat
     4681 cttgaggcgc gttgcacggt gctgatgagt cccgtttttg gtgaagtcga tgcgcgacag
     4741 ttggcggaat gggtgctggc tgatcgattg ccggtgaggt ttcagttgca gatgcataag
     4801 cagatatggg cccccgatat gcgtggcgtg taagccctgc ggattggccc cgtcggcaga
     4861 ggaaagaata gagaacggca agaaccaggg aggattgatg aagaagatcc aagttcaagc
     4921 acaggttggg cgggcagaac aagaaggtgc cgaggtgctg gtattgctct gttgcgaggg
     4981 ggcgtcagac ctttcgccgg aagctgccgc catcaacacc cagcttggtg gacagctcgc
     5041 gaccttgatt cagcgcggtg aattcgaagg caagcttggc gaggggttgc tcgtgcacac
     5101 gcagggcaag gccaaggcga agcggttgct cctggccggc ttggggaaag aaaaggattt
     5161 gcgcctggac gccttccgcc aggcattagg cgctgcagtc aaacgcgtcc gccaggccaa
     5221 agtgtcttca ttcggcgtgg ttctgccggg tgcggttctg gaagaaattc ctgttcagga
     5281 tgtggcccag gccatggccg aaggggccat cctcggcaac taccaattta cggcgtatcg
     5341 gagttcgaac ggaagcaaac cggtagaggt cgagcggctg acgatctacg tcgcacaaac
     5401 atcgctgctg gcgcaggtca ccgagggcat ccggcgcggc gtcgcgacgg cggaggccac
     5461 ggtcctggtc cgcgatctct gtaaccatcc ggccaatatc atgacgcccg gccggatcgt
     5521 gcaggaagca aaggctgtgg cgaaagaacc gggcgtcaag ctgaaggtgc ttgaacagaa
     5581 agatatggaa cagcttggta tgggggcctt gctcggagtg gcgaagggca gtcatgagcc
     5641 gccgaagttc atcattctgg agtacaaggg cgcgaaggca aagaaaggag atcccccggt
     5701 ggtcatggtc ggcaagacca ttaccttcga caccggcggt atttcactca agccggccga
     5761 gaatatggaa cacatgaagg ccgacatgac gggcggcgcc gaggtgctgg cgacgatgcg
     5821 agccgcggca cgactcaagt tgccggtgca tctggtgagc atattgcctg tggcggaaaa
     5881 tatgccgggc ggtcgcgcga tgcggcctgg ggatatcgtc aagacgcttt cggggaaaac
     5941 cgtcgaagtt caaaacactg acgccgaggg ccgactgatc ctctccgacg ccttggccta
     6001 tgccacccgc tacaagccgg ctgtgttgat cgacatcgcg acgttgaccg gtgcctgtgt
     6061 cgtggcgctc ggtcaatttg ccatcgggat gttcggtaac aatgatcagt tgaaggaaca
     6121 tgtccggaat gccggcatgc gagctggtga gcgggtgtgg gaaatgccgc tctgggagga
     6181 gtatttcgaa cagttgcgca gtgatgtggc cgatatgcgg aacatcggtg gccgtggtgg
     6241 aggcatgatt accgcggcac tgttcctgag caagtttgtc ggggactgcc cgtggatcca
     6301 tctggacatc gccagcaccg actggagcga gcgtgagcga gcctatctcc cgaaaggtcc
     6361 caccgggatc ggcacgcgcc tgctcattca attcttgctg gaccggaccc tgccctagcg
     6421 gcaaccggtg cgtgaggcgg cgtcagccga gcatctctct gcttctcacc cagatcgtca
     6481 tcgtaggggg ttatgacatt acgcgagcac atcggtcagc tgttcatgat gggatttacc
     6541 gggaccacgg tcagcaagga cctggcctcg tttctcaccg cctataagcc agggggcgtc
     6601 atcttctttc gtcgcaatct cgaatcggtc cagcagatcg tcgatctcac caacggactg
     6661 cagaagttgt cgccggcctc gccgctattg atcgcgattg atcaggaagg tggacgggtc
     6721 tcccgactgc cggccgaatt tacgatcttt cccccctgcg gtcagctcgg ccagtgtcat
     6781 tccagtgaac tcgcctattc cgctgcagcc accatcgcca aagaattgcg agccgtgggg
     6841 atcaacatga atatggcgcc ggtgctggac gtgaacagca accccgacaa tcccgtgatc
     6901 ggtgatcggg cgttcggtgc cgagcctgac gtcgtcgccg acatggggct ggccacgatc
     6961 ggcggcttgc aagacaatat ggtcgtggcc tgcgggaaac attttcccgg ccacggtgat
     7021 acggcaacgg attcgcacaa ggagcttccg gtcgttgacg cggggattca acggttacgc
     7081 gagacggagt ttcccccgtt tcagcaggcg atccggcagg gagttgccag tctcatgacg
     7141 gcccatgtgt tgtatcgggc cttggatccc gatgcgccgg ccacgctgtc tcccgctgtg
     7201 atccagcggc tactgcgcga agagtttcgt tacgacgggg tggtcttcac tgacgacctg
     7261 gagatgcatg cgattatcga tcatgacggg atcggcgaag cggctgttcg ttcgttcgtg
     7321 gccggatgtg atgtgttgct gatttgcaaa gaccaggacc gggttatgac ggccatgcag
     7381 gcgatggaac gtgctgtgca aaacggccgg atcacacaag aaagactgga gcagtccttg
     7441 gcccgtgtcg cacgactcaa ggcacgatac ctgatcccct acaagccggt gacgatctcg
     7501 gatgcccgtc tggtggtcgg gtgccgttcg cacaaggtgt tgctggactc atggcataaa
     7561 gcctatgccc ggctgccttc ctcgaaggtg tcggagcacg atgagccggc ggcggcgttg
     7621 gattcaccgg tcacgcatgt ctaacgcata gccgtatgcg ggtggggcat cgtctgaagc
     7681 agcggcgcga gggccaatct cctgagagga accggtgctg gattttcctc tcgagttttg
     7741 attcactagt gctggattgg gctccattcc atccattcat catactgagg cgccatgggc
     7801 atcaagaaag gcgacattct cgacgaacgg aaacgctttc ccgattcctg cgggctggtg
     7861 gttaagtgta ccggtggcgg cgagggtttt cagaagatct tttgttgtgg gcatgaactg
     7921 actgaagagg acctcgtgtc ggagatcgta tcgtcgaggg gccggaaacg aggaacctta
     7981 ctgccggggg ccatgctgga agagcggcga cagtttcctg attcctgcgg ccttcgcctc
     8041 atggtgatgg atggcggcgc cggactgcaa ggcatcgact gttgcggcca tctcattacg
     8101 gccggggcgg tgcatggctt gagattcggt caaccgctgg atgaaggggc tcctatgccg
     8161 accggtccgg cagggcaggc gtaatgcgcg cacccagtat gccgggggcg gatgagaagt
     8221 ttcatcgacc cagtatgcga tggtggctcg ggttcatgga tcagctggac cggcttggct
     8281 atgttgcggc cggattcagt ctgctggcgc tctgcctgat cattttcgtt catgcctggt
     8341 atatcttcct ggctcgaccc gttcagctcg cgctccttcc tgccgccctc aaattattga
     8401 acgatctgct cctggtcatc attcttttgg agctgtttcg cacggttgtg cgatttcttc
     8461 agacggaagt gctggaatta gagccgtatt tgtccgtggg gatcattgcc tgtacgcgaa
     8521 gactgttgac ggccagcgcc gaactctcct accagcttga agcggtggct aaggaaagca
     8581 ggcacgaatt atttcagcaa tatctcctgg atgtcggctt gaatgtgctg gtcatcatca
     8641 tcctcatcgg agggctgtac ctgcttcgct cccgtccgtc gaccgaacgt ccggccgtct
     8701 cctgagcggg agtggaccga atagcagggg gtgctaggtc cttcttgccc tcttcctatc
     8761 tcttatggca tcatgcacct cttcttggag gcatggtggc cacattgctt gaatatgtgg
     8821 gatcggtcca tatctacctc gggccctacc gaggaaatcc tattgccttg tatctgaggc
     8881 gtacggaggt gggatgtcag atcgggcctc gcgcctatcc atggaatgat gtgataggag
     8941 ccggggaaac tcctaacaaa gccgctgcgg atttcgagga aaaatggaag ggcaaggggc
     9001 tggctgctga tatgtattca ggcccgtcct gggaaggtgg aattaaaccc gaacggcctg
     9061 cccctccaaa acccgcggct cccccgaagc ctgcggccgc tcctgcgtct gctccagtca
     9121 cttcagctgc cgcacctgcc ccagctgcac cggcagcggc tgctgccaaa ccggccgccc
     9181 ctgctgcggc ccctgtcgca gccgcaccca aggtcgcccc agtcgcgcca accgaagcgt
     9241 cgtcttagtt tctccatcgt cgaagtgttc atgcagcggg gtcatgcgcg ccgagcgtga
     9301 ctggtgtgtt caagcgccgg acttgtggcg agtttgctgc gccgtatgcc gtacgtggcg
     9361 tagacaacga ttccgatgac cgtccacaca aaaaaccgga tccaggtcat ccagggaagg
     9421 aaatacatca agcctaagca ggccagcacg cccagcatgg gaatgaccgg catgaatggc
     9481 atgcgaaacg gccgaggatg gttcgggttg gtgtaacgaa gcacaatgat tccgatacag
     9541 acgaggacga aggcgaagag cgttccgatg tttgtcatat cggccgcttc gccaatggga
     9601 atcaaggcgg ccatgatggc cacggctatg ccggtaaggt aggtggcatg gtgaggtgtg
     9661 cgaaatttcg gatgcacgcc tgacagccag ggaccgagaa gcccatcgcg ggacatggcg
     9721 aaaaacacgc gaatctggcc cagcatcatc accaccagca cgctcgtgat cccggccact
     9781 gctccgaccg ctacgatggc ggccccccat ttgaagcccg cgacccgcaa tccttccgcc
     9841 accggggcat ggatgtcgat tcgcgaaaac ggcaccaggc cggtcaggac cgccgccacg
     9901 gcaatataca ggagggtaca gatacccagc gaggcgaaga tcccaatggg aagatcgcgt
     9961 tgaggatttt tggcctcctc cgcggtggtt gagacggcat caaacccgat ataggcaaag
    10021 aatacgatgg ccgccgccgc acctacgccg gcaaatccga acggcatgaa cggtgtccag
    10081 ttggtcgtat cgacggagga ggtcccgacg gcgatgaaaa agacgatcac ggccagcttg
    10141 atcaggacga tgccgcaggt ggctcgggca ctttccttta cgccgacgat gagaatgccg
    10201 gtgaccaata ggacgatgat cgcagccgga atattcgcga ttcctccgtc cgctcccggg
    10261 ggatgcgtgg cccagtaagg cagttcgagg ccacacagtt ttagaatgtt gttgaagtag
    10321 cccgaccagc cgatggcgac tgccacgcaa gccacgccgt attctagaat gagattccat
    10381 cccgtcagcc aggccaggaa ttccccgagg gtcgcatagg aataggtgta cgcgctccca
    10441 gccaccggga tcatggccgc aaattccgca taacagagcg cagcaagggc gcaggtgatg
    10501 ccggatagaa tgaacgacag gacgatcccc ggcccggctc ctggtcgatg cgcatcgccg
    10561 acgatcgcgg taccgatcag gacgaagatg cctgtgccga tgatcgcgcc aatcccaagg
    10621 ccggtcaggt cccaggcggt gaggcttcgt ttcagacgat gttcgggggc gtcggagtca
    10681 gcgaggattc gttcgatcga tttggtgcga aacagcggat tgcccacgcc gtgtcctctc
    10741 tccggtgacg tcactcccat gtgcgctggc atgggcgaat gagtcccctc tcatgtggtg
    10801 gtcccgcgct gcggagacaa tggtcgaagc gccggacgac gggaggctgc ccgtaagaat
    10861 gcctcaaaga gctgccgatg cagccggtgg cgctcaaaca gaaattctgg atgccattgc
    10921 aggcctagaa aaaatgggtg gccgggatgt tcaatcgctt cgacgatgcc gtcgggagcc
    10981 gtcgcgctgg cgatcaatgc gcgccccacg actttgaccg actggtgatg ggagctgttc
    11041 accttcatgc gcgggcgttt catgatgcgc tcgaggagcg tgccgggtgt gatggagacg
    11101 ctgtgagcga gctgaatagc tggtgtcgtc tgccggtgtt gaagtacatc ggggacctgg
    11161 gctggaaggt cctgatagag agtccccccg aatgcgacat tcatggtctg catcccgccg
    11221 cagatggcca gggtcggcag atgattgcgg gttgcgagat gaacgagatc caattcgaag
    11281 ctcgagcgtc gctgggcgac gatcggaaac gaatattgct tccgttctcc gtagagcgcg
    11341 gggtctaagt ctggaccgct gccggtgagc agcagcccgt cgatggtgcg gaggaggcgg
    11401 cgacgcgccg cacgatcagc cacaagggga aggatgagcg gcaccccgcc gagttcttca
    11461 attgcgcgga cgtaccgcgc ccggagaaaa taggtgggct ctttgccgcc ccattcctga
    11521 cgatcaccgg cattgaagtc gggcgtgact ccgatgacgg gtttcatact atcggatcgg
    11581 ttccaaaggg agcggcaggg gctcttgagg ggtatagtcc ccggcttccc gcgtgggttc
    11641 gctcgaggaa acaccgagga agcgaatcgg cttatccccc ttcagatggt ccggtgtgat
    11701 gatgtaggtg ccgtacatgc tggggatcca aatattcttt gcggtttgtt cgctcccgcc
    11761 tgagaagccg tacgccagtc cgccaaccac tccgccaccg atagcaaacg cgagtttgag
    11821 cgggaagtag atgatggttg ccagcgcgga tcctgcttga atgccgaccc ctgaagggct
    11881 ggcatcgttc gaggagacgg gaacgttgga tgcgctgccc gattcttgtg ctgccaccgg
    11941 cacaatgagg atggccagtg agcaaatcgc caccagaccg agaaccaacg cgtttgacca
    12001 gaggctgcgg cgtcttttcg aggacagatt gacagagacg ttcacgttaa gtgcctcctt
    12061 cgtgctgaaa tgctgacggt gtgaaattag aagatgagtg ccgatgaact tctacaatag
    12121 cgagtactcc gttgtcgtta taacaaattc atccccattt tcaaacccct tccgcgtttg
    12181 tcgcaggtgg tcctccgctc ttcaacgatc aggcgactct tccaggtcta attccaggcg
    12241 aatgcgctcc atcacctgtt cgggctgctc ggtcatgatc tgccggagtt catcggtaaa
    12301 gcgtgccgcg tcgatcacct ggttatgttg ctcaatgcct ccgtggagag cgagattgag
    12361 tgcgcaggtg catacggatt ggataaagag ttcatccgcc cgacgcgtca gctcagcctg
    12421 ttcctcttgg cagatcttgt gtaattgatg ttcaacccag gttgaaaagc cgatcgcccg
    12481 gcgcgcctcc tcgacgtgct tcatagcgat gtcgaggctg acctgtatgc cgcctttcca
    12541 actgcgtttt ttcacattaa acacgcagcg caggagatcg ggccgatgct ccaccggttc
    12601 gggtccgaaa aaaaatgtga cgcggtactg gcttggttct gtcgcagcgg ggggaatact
    12661 cataggtcgt ggaaagcatt ctatcattcc ggagcgcgga gagcatgtct attgtcgagt
    12721 ggtttcagtt tcgaaatgac gttatgatgc gatcatgaaa acgtctgctg atttcgtggc
    12781 tcaggtcgat cgtattcaag aagccattcg ggcacatccc gggattgacg ggtggctgtt
    12841 ttatgacttc cgccatctcg atcccattgc ctatcgcgtg ttgttgttag atccgtcgat
    12901 gcatgttacg cgccgttggt actattgggt gccggcggtg gggatgccgg tgaaattaca
    12961 gcaccgcatt gaaccgcatg tgttggaggc gttgcctggt gaggtgcggt tctatgtctc
    13021 ctggcgagaa cagcagacgg cgctaagggc gcttcttcaa tcggcgaaac gcattgccat
    13081 gcaatactcg ccgatgaatg cgattccgta tctttcgcga gtggacgctg gaacgttgga
    13141 tctcctgcgc agctggggtc tggagattgt tacctctgcc gatttggtcc agcaatttga
    13201 agccgtgtgg gatgaggcgc agttggcgtc gcaccaggcg gctgccacgg ggctgcgagc
    13261 gattgtggat gaggcgttcg ggtttatcgg gacatccctc gcggcaggct cttcgctcaa
    13321 tgaatacggg ttgcagcaat atatcctgtc tcggatgcat gctcgcggcc tcgtcacgtc
    13381 gagtcccccc attgctgcag tcaacgccca tagtgccgat cctcattatg ggccatctgc
    13441 cgagggctct gctcccgtcc gccaggggga cctcgtgttg atcgatttat gggctaaaca
    13501 gccggaggcc agggccgtgt atgccgatat cacctggaca ggtttcgtgg gcaaggtcgt
    13561 gcctgccagg cagcaagatg tctttcagat cgttcgccaa gcgcgggatg cagcggtgac
    13621 attcgtgcaa gcgcgcgttc gagagggcac atttccctac ggctgggagg tcgacgatgt
    13681 gtgtcgacag gtcattcaac aggctggata cggagagcat tttgttcacc ggactgggca
    13741 ttccataggt gaagaagtcc atggcaatgg cgcaaacatc gacaatctgg aaacgcagga
    13801 tgctcgccgg ttgctgcccg gtacctgttt ctcgatcgaa ccgggaatct atcttccggg
    13861 agacttcggt attcggagtg aattggacgt gtacctttcg accaatgatg cggtggtatt
    13921 cggtcaaccg gtacagaccg aacttgtcgc gatcaccctt cccaccacgt gagtctggtc
    13981 tcatcctgag ggatggcctc cgcctttctt gacacccttt gcatcgggca gctatcgtga
    14041 gagatcgact ggccgcagag gtctcgtgcc tggttggccc ctatttgccg aggaaggata
    14101 ccgaacatgt ttggcacgat gggattctcc gaactgatta tcatcctggt tatcgtcttg
    14161 attatttttg gtgccggcaa acttcctcaa atcggggagg gcgtgggcaa ggccctgcga
    14221 gggtttaaga aggaagtgaa tgatatccct gctgccgatg ccactcctga acagggtgaa
    14281 gctgcaactc cttcgtctca ggcacagata ccgcctgttg cagaagttgc ccagactgct
    14341 cagcccgcgc cggtggctca ggccgctccc agtctcaaac ctaccgcgcc ctacacgcct
    14401 ggtccggagt taacacctgg gacgacagca gccttgatgg cggctgctgc gcctcagggg
    14461 cctcaaacgg cccaagcgtc taagccccgg gtcgccacgg tcacgccaac acaggctgcc
    14521 cccggctcgg tggtggcaca ggggcatcaa cctccgacga tggaagatcg catggcggct
    14581 ccggcaccgg tgatgcgggg acagtatccc cctcttcctg cctcggctca gagtaagccg
    14641 gtggccaagc gtccttctgc tattgttaat aaagacgcag tggctcgggt acaggctgcg
    14701 caggctgcta tccgggcaaa agcagcgcag cctcagccgg ccgccttttc tccgcaagac
    14761 atgcaggggc ttggcgaggg actgggagac gccgtgcgga ctttccggca ggcggtcgcg
    14821 gatgtgcgga gttcggttga tcctcagatg cggaccattc gagcagaaat ggattccgct
    14881 caaaaggaac tggaacagtc gatcgaggca gcgaagcaag cgcccgtgct cgacgaggac
    14941 actcctgcca agccgctcta accgcgtgga gtttggatag ctttcgtttg gatcggtaat
    15001 gggatgtgtt gccccagggg acttacagct tgatgcgtgc agggtctata tcgtcgtgct
    15061 gggccgatcg acataccact gtgaaggaaa ttgtcacctg attcccggtc tattcctctc
    15121 gtgcagcatg ccccgaccat tttaacgctc acatgagttc cgaatcattc ctccgtaacc
    15181 cgtcctacct cgcattgtta ttgttgttgg tagtttcctt gaccatatcc ggttgctatg
    15241 tggaagtccc gccagcgacc cccgcggtgg tttccgtgag gctgaacgaa ttactcgctg
    15301 atcccgatcc ggaagtgcgt cgcacggcgg cagaagggtt gggaaagatc ggccacccat
    15361 cggcaagccc agggcttatt gctgcgttgg cagatcgaga tccccacgtt cgcgcagctg
    15421 cagcactcgc cttgggtcga gtgggtgatg caaagagtgg ggcggcgctt gtggaacatt
    15481 tgtcggattc gtctgagtct gttcggactg cctccgcctt ggcgcttggc gaaattggaa
    15541 cttccgtcaa tcatgaggag aagattcttc gcgcactccg ccagccggag gtttctaccc
    15601 ggctggctgc cagtcgagcc ctcctcagtt tggagacggt ttcattttca acggatctcc
    15661 tgtcggccct ggacgatccc aatgcgcagg tgcggcaagg ggttgtagcg gttctcagtg
    15721 agacagggga taggagacct ctaccaagtc tgatgaggct tttgcaaacc gatgaggatg
    15781 cgggagtgcg cgctgaagca gcgtttcggc tgggaaaagt gggagatgcc ggtgtggtgc
    15841 ctgcgttgtc cgtggtagcc gagaaggatt cgaatccgac ggttcgacaa tgggcgcatt
    15901 gggctgttcg gcagattacg acatcgcccg gcttcggttc agggaatcga caaggtcaat
    15961 gaggcgagtc tgagccaccg gatcaatgtc ggtaaactga atgcccatac ctggaaagag
    16021 cagataccgc tctggcttcg accgtgtcca ggctactttt gcacgtgtgg caaatttctg
    16081 gttcggtcgg tcaggtaggg caaattccac ggtcagttct gtgcccggtg ccaacggcgt
    16141 actactttcg ataaacaatc cccctccgcc gatcccgcca gtcaagctat caaaatgctt
    16201 cccgtcctcc gtggtgcaat ggactttgat ggcaagggga gcgcgtggat gcgatcgact
    16261 atgggcaaag cgggcatctt catcaacgga gagcatatgc tcgatgaggg ttccccagct
    16321 taggatactg agctgttgtc ccttgtgatc gaacaacgat acggtttcct gttcgcaatc
    16381 aataccgagc gttttgccgt ggtgctctcg agtggcagtg acgggaaatt tcatcggtga
    16441 ggtctcggag ggtccgcagt gaatcgatct gtcacgaatt ggattcgtgt gtcgagagtg
    16501 tttagcagga ctactcgctc tgcggaatgc cttttacaga atgaaccaga ttcagcacgc
    16561 gacttcgggt ctctgatgcg atatctgtga aacgtacacc cataccaggg ctaaaagtat
    16621 actggtcggc cttggggcaa acccaggcga tcactccctt ggcggacatc cactcgctgg
    16681 ggtcttctgg tagcgtgaat tccatggcaa gtttcgttcc gactgccaag ggggcgaggc
    16741 tctcgataaa gagtccgcca ccgccgatgc ctccggctcg actctcgaac tgcatgccat
    16801 cgggggtgtg gtatttgacg cggatagaaa gggagatccg cggttcagaa cgggcttcac
    16861 gggattgctg gggctttcga taggtcagga tctgatcgac gagaaaatcc cacgcgaggc
    16921 ttcccattgg ctcgccgttg atgccaagca acgtgatcga ttcctttgag gaatcaattt
    16981 caatcgtctt gcccttgtgt cttgtgctgg atacgacggg gaatttcaca agccgccctt
    17041 tacaaaccta catgttgcaa caaggcatct ggtgcaagta tagcccatgg atcgaacttg
    17101 tcgataaaaa cctgcgattg gagaagtttg gcgaaggaga ggtcggtaat ctactgcgaa
    17161 ggggaataaa aaaaaagagg gggcggacgc cccctcccga tcgctgaaat gcgcttaata
    17221 gacgagtagt tttgaggggt taggcgctct tgacgtcttt gtcctgttcg aaaatccgaa
    17281 taggcgcgtg ttttccgctg atggcatctt cagtgatcac gacttccttg atctgtttct
    17341 gcgacggggc gtcgtacatc acatccagca tggcctcctc gagaatcgca cgtaacccac
    17401 gggcaccggt cttttgagta aaggctttcc gggctacagc acttaacgca ccttcagtga
    17461 atcggagctt cactttttcg aaggacagca gcttctcata ttgcttggtc agcgcatttc
    17521 tgggctcggt gagaatccga atgagcgctt gttcgtcgag ttcatcgagc gtagcaacga
    17581 ccggcaaccg accaataaat tcggggatca atccgtactt caacagatct tccggctgga
    17641 catgcgggag cagttcgccc aatcgaatat cattgcggcc ccggacctcg gctccgaacc
    17701 ccatcgattt ccggttcaga cgctgctcaa taatgtgttc aagcccgaca aatgcaccgc
    17761 cgcagatgaa caaaatattg gaagtattga cctgaatgaa ttcttgatgc ggatgtttcc
    17821 gcccgccttg aggggggacg ttcgcgaccg ttccttcgat gagcttcagc aaggcctgtt
    17881 gtacaccctc gcccgaaacg tctcgcgtaa tcgacggact gtcacttttt ctgctgatct
    17941 tgtcgatttc gtcgatatac acgattccgc gctctgcccg ttcgacatcg taatcggcag
    18001 cctggagtaa tttgagaatg atgttttcta catcttctcc gacatagccg gcttccgtaa
    18061 gtgtcgttgc gtcggccagt gtgaagggaa catcaagaaa tttggccagc gtctgagcca
    18121 gaagggtttt tcccgtgccg gtcggtccga cgaccagaat gttgcccttt tgcagttcaa
    18181 cgtcgtcgac gtctttgtcc tttgcggaga tgcgcttgta gtgattgtgc acggcgactg
    18241 agaggatacg tttcgcacga tcctggccga cgacatactg atcgagatga tgtttgatct
    18301 cggccggctt cttcagcttg gaggagattt cctccttcgc ttcttcccag tcctccgcaa
    18361 tgatgtcatt acagaggttt acgcattcgt cgcagatgta gacggtagga ccggcgatga
    18421 gtttgcgcac ttcgtcccgg cttttaccgc agaacgagca acgcaggtgc cgatcaatct
    18481 tttcctgttt agccatgctg cctcctgtac accaaaggtc aactagctct tgccgccatc
    18541 cttccctgag tccgttgagc ccacgggttt aaggctcttc agggggcgtg taatcacttc
    18601 gtcaatcagg ccgtagcgtt tggcttcctc actggacata aagtagtctc gctcggtgtc
    18661 ctgggaaatc ttatccagcg gttgtccggt atgtttcgcc atgatttcat tcaggcgttc
    18721 acgaatcttg aggatctccc gggcatggat gtcgatctcc gttgcctgtc cctgaaatcc
    18781 gcccatgggt tgatggatca tgacgcgggc gttgggaagt gcatagcgct tgcctttagt
    18841 cccggctgtt aaaagaaacg cccccatgct ggcagcctgc cccaagcaga tcgtgttgat
    18901 ggatggtttg acgtattgca tagtgtcgta aatgccaagt cctgccgtta cgcttccgcc
    18961 tggcgaattg atgtacagat taatatcctt ctccgggtcc tctgcctcca ggaacaggag
    19021 ctgggcaata atcaggttgg cgaacacgtc atcgatagga gccccgagga agatgatacg
    19081 gtccttgaga aggcgtgaat aaatgtcgta ggcacgctcg ccgcggtttg tttgctcaac
    19141 aacaatcgga actaacattc ggccgaatcc tttccgctag aggactaaca ggtggaaact
    19201 cctcagttta ccatactcat cctgccggga attggtgatg accgcccgac ctcagtaccc
    19261 gcgactcctg atttaagagc gtttagccct gaatcatcgc gtgcttgtat acaaaatcca
    19321 atgctttgtc agccaagatt cttgaccgaa ggtcattcag ggtgtcttga ccgccggctt
    19381 gaatcatgcg ggtgacttcc tcgacggaga gtttcacttc agcagcgagc cgcgcgattt
    19441 cgttgctgag atcttcattg gtcaccgtga tgttttcttt cgttgccagc gcttccagaa
    19501 tgagcccgac cttcacgcga cgctccgctt ccggacgata ttcttcctgc agctgtttgg
    19561 cttcgtcggc ttgagaggca ccgggcgatc cgtctgccgc gtcaccggtc ttccgttgac
    19621 gagactgcaa ttgttgacga accatcgcag agagttctcg ttctactagc gttccgggaa
    19681 gatcaaaatg gtgggtttcg gccaatcgct tcaaaatcgt atctttgtag gagtcctcaa
    19741 tgtccttttt caaggcacgg tccatttcgg cgtggagctt ctctcggatt tcctgcagag
    19801 attgatacgg gccgcaatct ttcgcgaatt cgtcgtccag ggccggaagt tgtttgcgtt
    19861 ttatggactt gacggtgcag cggaagttga cggtctttcc cgccactcgc gcatcgggat
    19921 gtgtcgccgg gtaagcctgg ggaatggtga tgacgtcacc ctcctttttc cctaggaggt
    19981 gcggttcaat ttcgattccg aggacggaag actgagaacc cattttatgg agatgtcccg
    20041 tcttggttgt tccatcgagc gcagcaccgt ccactgtgcc gtcaatgtcg acaaccgcat
    20101 agtctccatc ggccagagtc gttccggctg gcgcgggatg caactgagcc atctgctcac
    20161 gaagaacctc aaatgccttt tgcacctgtt catcggtgac tgttctctga tcctgtttta
    20221 gagagattgg attcggagct ttgtaatccc gaagctcaat cgttggctta atctcaacgg
    20281 tagccgtaaa gctaaagggg gagtctttct tcactttgac ccgttccaac ggcggtattt
    20341 ctacaagaac cggaacgatg cccgcctgcc ggatagctcg atcataataa tccggcacca
    20401 agctacgaat gacatcttct tcaatcgttt tggcgtagcg cttctcaagc aattggaggg
    20461 gggcttttcc cggacggaag ccgggaatgc gaacttgccg attcaattca gagtacgcct
    20521 ggacaaaccg caggttcact tcgtctgcag gaacttcgat tttgagcgct cgcttcatgg
    20581 ggccgagttc ggtcatttcc atcttcataa ggctattcac tttctccgag tccgatagtg
    20641 gttggtgcgt catgccaggt catagaggtg gtgcgagagg ggggacttga acccccacgg
    20701 ttacccacga gatcctaagt ctcgcgcgtc tgccagttcc gccact
//
LOCUS       DAOQEW010000052        19881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_689_length_19881_cov_8.170281, whole genome shotgun sequence.
ACCESSION   DAOQEW010000052 DAOQEW010000000
VERSION     DAOQEW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_19881_cov_8.170281"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /gene="argC"
                     /locus_tag="PLS35_07735"
     CDS             <1..1077
                     /gene="argC"
                     /locus_tag="PLS35_07735"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNI18587.1"
                     /translation="KMTSVRVAVAGASGYTGAELVRLLSQHPHVQLTAVTSEKSAGMA
                     ISAVYPHLQGIVQLPFEALAPEALAERADVLFLALPHTKSMGPVASCMKAGKRVIDLS
                     ADFRLKNPATYETWYQTAHAQPQLIKDAVYGLPELHRAAIAQARLVASPGCYPTAAIL
                     QLAPLIAHGLVSPDSIVIDAKSGISGAGRSPALPYHFPEAHESLEPYKIGQHRHIPEI
                     EQELTGLAGSKGRAAEAGQGPVTVAFTPHLVPMNRGILSTAYARMTNQPDVAELRNLY
                     REFYKGERFVRLLEGAMPNPRHVRGANYCDIAVQTDRRAGWVVTVSAIDNLIKGAAGQ
                     AIQAMNLMLGYPEETGLIAPGVYP"
     gene            1135..2346
                     /gene="argJ"
                     /locus_tag="PLS35_07740"
     CDS             1135..2346
                     /gene="argJ"
                     /locus_tag="PLS35_07740"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNI18588.1"
                     /translation="MKTISGGVTAPIGFRAAGVYSGIKKKKKKQLDLALLVSDREGPV
                     AGVFTKNQVVAAPVILDRLHLKQGVGRAFLVNSGNANACTGPEGMQAAKEMAKLAATA
                     LNCAPQTIFVGSTGVIGQPLPIDCIRKALPALSARITRRGGAEAAAAIMTTDLKPKHV
                     AVRALLGGKMVTVGGMAKGSGMIHPNMATMLGYLTTDAAIGQRALQAALRQAVDQSFN
                     CITVDGDTSTNDTVLCLANGMADNRPITPGSADFTRFCAMLETVCRTLALKICWDGEG
                     VTKVVRVEVKGAQSTKAAKQVAQTVATSNLVKTALFGGDANWGRVMAALGRAGVPIDP
                     SRISLQFGGVPMVRKGQGLGRAAERQLTKVFKAKEFTILIDLAQGKAGAHMWTTDLSY
                     EYVRINASYRS"
     gene            2576..3349
                     /gene="rpsB"
                     /locus_tag="PLS35_07745"
     CDS             2576..3349
                     /gene="rpsB"
                     /locus_tag="PLS35_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNI18589.1"
                     /translation="MGVVAIKELLEAGVHFGHQTNRWNPKMKRFLFGERNGVYIIDLQ
                     QTVERMEQAYAFARDTVAAGDSVLFVGTKRQAAEILEEEAKRANQFYINQRWLGGMLT
                     NFQTIRRSIDKMKKMEATLADPTHQGHTKKELGQMQKEVVKLQKNLSGIRNMRALPGA
                     VFILDTRIEHIAILEASRLEIPVIAIVDSNCDPDHIQYPIPGNDDAIRSIKLIISRIA
                     DACLEGAHLRAQQEETEFASAPAGGAAKPAPKLAGVSAS"
     gene            3401..3997
                     /gene="tsf"
                     /locus_tag="PLS35_07750"
     CDS             3401..3997
                     /gene="tsf"
                     /locus_tag="PLS35_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNI18590.1"
                     /translation="MASLSELVKELREKTGAGILDCQKALTENGNSIDKAIDYLRQKG
                     LAAAQKKAGRETNQGLIHAYIHAGGKIGVLIEVNCETDFVARNEQFKAFVNDLALQVA
                     AASPSYVRREEIAADVVAKERSIYEGQAKELGKPPAAWPKIVEGKLEKFFQENCLLEQ
                     AFIKDPSVVIKDLLAQQIATIGENMNIRRFTRFQLGQA"
     gene            3994..4719
                     /gene="pyrH"
                     /locus_tag="PLS35_07755"
     CDS             3994..4719
                     /gene="pyrH"
                     /locus_tag="PLS35_07755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNI18591.1"
                     /translation="MSSAHYRRILLKVSGEMLAGEQGYGIQPSILEGLAEEIADVVAM
                     DVEVAVVIGGGNIFRGLAASARGMERASADYMGMLATVLNALALQNALEGRGVSTRVQ
                     SAIEMRQLAEGYIRRRAIRHLEKKRVVIFAGGTGNPYFSTDTAASLRAMEIGAQVIMK
                     GTKVDGIYDSDPVKNPQAKKYNEIPFLSILTQNLKVMDSTAISLCMDNRLPLIVFNLK
                     EKGNFKRVVQGDPIGTLVTVESR"
     gene            4731..5294
                     /gene="frr"
                     /locus_tag="PLS35_07760"
     CDS             4731..5294
                     /gene="frr"
                     /locus_tag="PLS35_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNI18592.1"
                     /translation="MSTAQEVKQRVTEKMDHAIEHLKRDLAGIRTGRASVALLDGIKV
                     DYYGTLTPLKQVANVATPEARLITIQPWEQNLIREIEKAIQMSDLGLTPSNDGKMIRI
                     PLPPLTEERRKDLIKVCKKHGEEMKVQIRGFRRDGNEELKKLQKDAKLTEDELRKSET
                     EIQKFTDQYVQKIDDMMKKKEGEILEV"
     gene            5321..6484
                     /gene="alr"
                     /locus_tag="PLS35_07765"
     CDS             5321..6484
                     /gene="alr"
                     /locus_tag="PLS35_07765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNI18593.1"
                     /translation="MYQPSESSPTSASVDLTALAQNVAHVRRLAPRADVLAVVKANAY
                     GHGAVEITRALQQLAVRRFGVATVDEGVALRQAGIRDDIVVLGATMPAQFPDLVAHRL
                     TPVLYRADLLPAFAATVKTGTAPYPVHIKVETGMGRLGVLPRELGELVSSPELRGPLQ
                     LEGLMTHLADADNPDPEQTEEQLARFQQVLPQVQERGFAIPLIHVANSAGIIKYPKSL
                     HSLVRPGIMLYGYHTLANDRQGPELQPILTWQTTIAHMHTIPPGSSVSYNRTFIASRQ
                     TRVAVLPVGYADGYNRLLSNRGQVLIGGQRVPVIGRVCMDMTMVDVTDVQGVQVGQEA
                     ILIGQQGGERITAADLAAWQQTIPYEILCAIGPRVPRRYRPLMASTDSASRTS"
     gene            6632..8053
                     /locus_tag="PLS35_07770"
     CDS             6632..8053
                     /locus_tag="PLS35_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNI18594.1"
                     /translation="MICWIPLTQQHLMQWFALVLLAAPVTSAIAAGTQASAPDGTKPS
                     AMTIHIPIPVTPLAELYGNVNQQLAAHRSRGREWVTLGGGSGFLKYRLWPDEQGSTTA
                     GDRLLSQSTVPFGVEYAKQIKGSITKIAECGQRDASTGTGRLSVTVATMFKQGHSYTV
                     VPASQVSAVQSAQSCVLSEQGVDASPLMVQVYRSDLMEVLPAIDRKAASLVPIKPAVA
                     RIWNDLQEPMLLDETEQLWLMLNPDTTAAAGVAPLSGSPAAGYGVIARPTVVRGTKPA
                     SRHLPLPEPQDHFRDNGFHVDFDLQVPIEEANQRLREAVIGQEWSLGVGTIKIVNATL
                     YPLGHQVGVELVLRGLLPLTLRLKGTPAYDESAGRILFREVDYTIKERTPATDLAEEW
                     LHEPLREELAGRLSLPIREELDLMRQALEKGLNRELTGGRLRGTVRQLSLEDLAVQTA
                     SLSVRFKTEGTLRYDARPDVTAP"
     gene            complement(8060..8368)
                     /locus_tag="PLS35_07775"
     CDS             complement(8060..8368)
                     /locus_tag="PLS35_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNI18595.1"
                     /translation="MVLLEFSMSPLGKGESVSKYVARSLDIIDKSGVDYRLNPMGTVL
                     EGEWDEVMAVVKKCYMRMRKDCNRVSCSIKIDYRKGPKGRLSGKVSSVEKQLKRKLKI
                     "
     gene            complement(8385..9044)
                     /locus_tag="PLS35_07780"
     CDS             complement(8385..9044)
                     /locus_tag="PLS35_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNI18596.1"
                     /translation="MAANSGFQISLDVRGWPVLVIGGNEEAAEKVQRLLDAGAKVTVV
                     SPTLHEMLRKLAASAKIIHRGRHFRANDLESVILVLNTLRDDRELAHSLIRLAREQKF
                     LLWSVDQPDVSNVVMPAVVSSGHVRVAISSSGQAPALSGFMKEDLERILDSEFAAFVD
                     WLAQLREQAKANEPDAEKRRALLREALDGFRLLGKVQYPKVWQEQRASQQAPAAPAST
                     T"
     gene            9217..9669
                     /locus_tag="PLS35_07785"
     CDS             9217..9669
                     /locus_tag="PLS35_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18597.1"
                     /translation="MAGYTGRSLAMAGGIIGLAAVAAYFGVAHIGEPDQGGGSIAGQV
                     QIAPNLADQVKPTDVLFVIVRRPTGPPRPIAAKRFDGPKFPVQFEITNDDVMVKGNEL
                     KGMVDVIARLDRDGQAGQPQPGDMEGRYPKNPTLPGGRDLMITIDKVY"
     gene            complement(9682..10227)
                     /locus_tag="PLS35_07790"
     CDS             complement(9682..10227)
                     /locus_tag="PLS35_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNI18598.1"
                     /translation="MEMTLLLNSTYEPLRVLHWQKAITLLWQGKVEVLEVYDRQIHGI
                     SISIKLPSVMRLLKLVKLKDSHRAVKFSRINIFTRDGYCCQYCKHKFRTEELTFDHVV
                     PIAKGGRKTWENIVTACWRCNNRKSGRTPEEANMRLIKKPVKPRWSPTVTITIGIRNT
                     PESWRDYLYWNLELDADSPDT"
     gene            10426..10752
                     /locus_tag="PLS35_07795"
     CDS             10426..10752
                     /locus_tag="PLS35_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNI18599.1"
                     /translation="MNSYDGYVTVAQADDIPPGACRTVEVDGIFLALSNVDGTFRAVD
                     NTCPHAGGPLGEGCLDGELIECPWHGWRFNVRTGERPENPEIRVTCVEVRVLGTNVQV
                     KVPLTL"
     gene            complement(10742..11197)
                     /locus_tag="PLS35_07800"
     CDS             complement(10742..11197)
                     /locus_tag="PLS35_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18600.1"
                     /translation="MSKVIHYAVLLLLVVGGLVYWWMKPPGINPITDPRAAEALALVQ
                     THRAAGYPTILQAFNERARILADRKLGLRLGEWQVIKQDEQRYEVRIYMREQGTTQWF
                     ERDFIWHVDLGTKRVNAASLPADGLMPEGPDSGRAGTPPAGAPPFPPTM"
     gene            11401..11844
                     /locus_tag="PLS35_07805"
     CDS             11401..11844
                     /locus_tag="PLS35_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNI18601.1"
                     /translation="MKFFFYADNLNPSQLKRRAPEHKFLFTAYLPDHSVQFCRWSTQW
                     RCGLASVVPSPGEKVWGAVFEITDEDLKILDEFEDDVPQGAYRHLPVTVITEDGEKLL
                     VTTHAANPIGKFKPKEHYIDWVLKGIKQWKLPEECSEQWKAYKPA"
     gene            11888..12214
                     /locus_tag="PLS35_07810"
     CDS             11888..12214
                     /locus_tag="PLS35_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNI18602.1"
                     /translation="MPKPKHHILVCTNARPPGHPKPSCGGQGSAQLLMNFNMGLMQRG
                     IMPGEVLVTGSSCLGPCEQGPTVVVYPEGTWYSKVTEADVATILDEHIKGGKPAEKLN
                     PDSIWK"
     gene            12421..12876
                     /locus_tag="PLS35_07815"
     CDS             12421..12876
                     /locus_tag="PLS35_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNI18603.1"
                     /translation="MIITCSDTRTPDTDTSGQLIQKLLKENGHTIAAYYLVKDEPAQI
                     KARITEGVANDSVQAIIINGGTGISRRDSTFEAVDAMLEKRLDGFGEVFRFLTYQDIG
                     SPAIMSRATAGIINGRVLFSTPGSENAVRLAMEKLILPELGHLVKELTK"
     gene            13097..13270
                     /locus_tag="PLS35_07820"
     CDS             13097..13270
                     /locus_tag="PLS35_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18604.1"
                     /translation="MKREGSRKPLAPSARPRILKTVIQRGRRRSKTGGLPVSYVKDFD
                     ETRTLLEAMSNEG"
     gene            complement(13524..13958)
                     /locus_tag="PLS35_07825"
     CDS             complement(13524..13958)
                     /locus_tag="PLS35_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI18605.1"
                     /translation="MATVAQIMNKKPQSVGPTMSLRGAAKKMRTLRVSSLLIKKGKNH
                     VGIVTDTDLVRKGLATNQDVGKLTVEQVMTSPLCTIESNQAVDDAQDMMGDLGVRHLA
                     VTKGGSIVGVVSVRDLLMHYKRYAQSRLADKQVYSEPKIGQD"
     gene            complement(14009..15040)
                     /locus_tag="PLS35_07830"
     CDS             complement(14009..15040)
                     /locus_tag="PLS35_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNI18606.1"
                     /translation="MNAVVFHEHGGPGKLQYQEMPMPTIGVDEVLVQVKACALNHLDI
                     WIRQGSPAYPMPLPHILGSDIAGVVHQIGSHVEGVAVGERIYVSPGVGCGKCEQCLAG
                     RDNMCRSYGLIGAMCHGGYADYVKVPARNVRPIPGALSFEQAAAFPLVSVTAWHMLFG
                     LAEVQPGEEVLIMGAGSGVGHMAIQMAKLAGARVLTTVGSEEKVAKAKDLGADEVIIH
                     SREQVNQRVRELTERRGVDVVIEHIGPEVWTQCIDSLAKGGRLVTCGATTGAEVKLDL
                     RYVYSRQLTIKGSYMGSQSELLKAAHLVGEGKLRPVIDRTFPLSEAKGAQEYLLNRKF
                     FGKIVLTVS"
     gene            complement(15249..16178)
                     /locus_tag="PLS35_07835"
     CDS             complement(15249..16178)
                     /locus_tag="PLS35_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNI18607.1"
                     /translation="MPGQRRLQFYQADVFTDEPFGGNPVAVFPDADGLTDVELQQIAR
                     EMNLSETVFVFPPTDQAAVVKMRIFTPTQEIPFAGHPVIGTFFVLGTLDRLALREPVT
                     RVLQECNLGLFPVDIHSHDGVIERVVMAQPSPEFLDVIEEPEALFAIARALGITKAAI
                     TESRFPVQVVSTGLPVIIVPVRTLTAVRSIVPDVAAIAELSQQYGANGMMVFSTMTVE
                     QSSTVHTRMFAPLIGIVEDPATGSASGALGAYLVQHGVVDIRPETEITAEQGYEIDRP
                     SRILIQVNSDDDAIQGVMVGGEAVMVVEGVLTF"
     gene            complement(16650..17891)
                     /locus_tag="PLS35_07840"
     CDS             complement(16650..17891)
                     /locus_tag="PLS35_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18608.1"
                     /translation="MPTTLPIFQPTPIFKDRTDYRQVLVREMDAGKIPLSLGRDCPVK
                     CEFCYELDHSYRETLDPPKTSDEDWKFILEYISRKPTDPTQFWCLGGNEFMEWTDLFL
                     HPKAMEWVEDFLKYTDKSIQFFTVGFVHVPKIHQLVAQYPGRINFELSVITLSDYRQR
                     LMPHAPSVKHLMKVLDGPAVSSANFYAFDQHTMSKDAATISAINQQCVLWMGTLTPVR
                     GLKEETASLMRQGRKFLPDEATRIYHLGLPNLQTIHTESYTTAFLSRRRIVSLFDSLE
                     LEKKDTVVMAGSVHKILTMFRKNRAKFLYVPNALLSGDSDCTVLLTFDDIARRLTKEK
                     AIHVPKCIMQSGRGPYTDITGVSLEQFTKKTGVRVKVLHKIDTRFANQQLYRHGSLQN
                     YVEQYLNHPMRAAYEAIPRPA"
     gene            17974..18237
                     /locus_tag="PLS35_07845"
     CDS             17974..18237
                     /locus_tag="PLS35_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18609.1"
                     /translation="MAEKRFTEALALFALTEPFTREQLDQKRDALLHTWTPRRFANLT
                     NNPKKYMQAYTKAEEMTRLIETSYALLAAIVVPDEARRAPRGT"
     gene            complement(18454..>19881)
                     /locus_tag="PLS35_07850"
     CDS             complement(18454..>19881)
                     /locus_tag="PLS35_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI18610.1"
                     /translation="SKEVFACLDDEQVNVYRDICNQLVLPSVSKEKPSVIGISIGTQM
                     QLMAGLTFCKMIKESFPEIRVVVGGNVVTRLQEEWAHHERFFTEVFDAAILYEGEHAL
                     LWYLEAVNGQRAIDQVPNLMYRDADGVKQSKEVYTEKTSALPLPDFDGLPLDNYFVPE
                     RIIPYLATRGCYWGRCTFCDHGQGYFDQYRGMSAQHVVEQVTALRDKYNCRHFLFSDE
                     SYPPALFKKVSQLLVDRNVGIKWTTLIRFEETLQDQAIWDLAAKAGCCTLYYGMESAN
                     ERVLNLMDKHARKSVIQNNLHQAAKAGIWNHVMAFYGFPGETRDEALETRQFVIDNQP
                     VIHSVELFYFVAYRHTPMVRNPDKFGITIHKQEEYDLPLDYYYTLNEPVGISCLDAMQ
                     LCEEFYKNDFQPWAVRVNSREHVFLYISKFGTNKLPQIYAATKDGAATEGVSGLVTWP
                     MAVAEGAGEKDGETSMSRVVSHGVE"
BASE COUNT         4417 a         6003 c         5606 g         3855 t
ORIGIN      
        1 aagatgacgt cggtacgagt agcggtagca ggggccagcg gatataccgg agcagaacta
       61 gtgcggctcc tctctcaaca tccccacgta caactcacag cggtcacctc ggaaaagtca
      121 gcaggcatgg ccatctctgc cgtgtatcct cacctgcagg gaatcgtgca actcccattc
      181 gaagcgttgg caccggaagc gttggcggag cgggccgatg tcctgtttct ggcactcccc
      241 cacaccaaat cgatgggtcc cgtggcaagc tgcatgaagg ccggcaagcg tgtcatcgat
      301 ttgagcgccg acttccggct gaaaaatccc gctacgtatg aaacctggta ccaaaccgca
      361 catgcacaac cccagttaat caaggatgcc gtgtacggtc tgccggaact gcaccgcgct
      421 gccattgcac aggcccgact cgtagcctca cctggctgtt accccacggc tgcgattctc
      481 caactcgcac ccttgatcgc ccatggattg gtctcgcctg acagcattgt gattgacgca
      541 aaatccggca tctcgggcgc aggaagaagt cccgccctcc cctaccactt tcccgaagcc
      601 cacgagtcgc tggagcccta caagatcggg cagcatcgac acatccctga aatcgagcag
      661 gaactcaccg gattagccgg cagcaaggga cgtgcggctg aggctggaca gggccctgtg
      721 actgtggcct tcactccaca cctcgtcccc atgaatcgag gcatcctcag caccgcctat
      781 gcacgaatga cgaaccagcc cgatgttgcc gagttacgga atctctatcg ggagttttac
      841 aagggcgaac gatttgtgcg cctgctcgaa ggcgccatgc cgaatcctcg ccacgtgcga
      901 ggagccaact attgcgacat cgccgtacaa accgatcgac gcgccggttg ggtcgtgacc
      961 gtgtcggcca ttgataacct gatcaaaggg gcagccggcc aggccattca ggccatgaat
     1021 ctgatgctgg gttacccgga agaaaccggc ctcatagctc cgggcgtcta cccctagcgt
     1081 gcacttgcat gggcccgtca tagcgagtca ccttcacatc acataggaag agcgatgaag
     1141 acaatttcag gaggcgtcac cgcgcccatc ggttttcgag ccgccggagt ctacagcggc
     1201 atcaaaaaga aaaagaagaa acaactcgac ctggcactgc tcgtgtcgga ccgggagggt
     1261 ccggtagccg gagtcttcac gaagaatcaa gtcgtagcag cgccggtcat tctcgatcgt
     1321 ctgcacctca agcagggcgt cggccgcgcc ttcctcgtca atagcggaaa cgccaacgcc
     1381 tgtaccggcc cggaaggcat gcaggcagcc aaggagatgg cgaagctggc ggcaacggcc
     1441 ctcaattgcg caccacagac cattttcgtc ggatccaccg gggtcatcgg tcaaccgctc
     1501 ccgattgact gcatccgaaa agcgcttccc gccctgtctg cacgtataac acgtaggggc
     1561 ggagccgaag cggccgcggc gatcatgacc accgacctca aaccgaaaca cgttgccgtc
     1621 cgggctctac tcggcggaaa aatggtgact gtcggcggca tggccaaggg atcgggcatg
     1681 attcatccca acatggccac gatgctgggc tacctcacca ccgacgcggc catcgggcaa
     1741 cgggcccttc aagcggcgct ccgacaggcg gtcgatcaat cattcaattg catcacggtc
     1801 gacggagata ccagcacgaa cgacacggtg ctctgcctgg ccaacggcat ggccgacaac
     1861 cgccctatca cacccggctc agccgatttc acacgcttct gcgcgatgct tgaaacggta
     1921 tgtcgcacac tcgcgctgaa aatctgctgg gatggcgaag gtgtgacgaa ggtcgtacgg
     1981 gtggaagtga agggagcaca gtccacgaaa gcggccaaac aggtcgccca aacggtcgca
     2041 acttccaatc tggtcaaaac cgcgctcttc ggcggcgacg ccaattgggg ccgcgtcatg
     2101 gcggcgctcg gacgtgcagg cgtgcccatc gacccttccc gcatcagcct gcaattcggg
     2161 ggggtcccga tggtgcgcaa gggacaagga ctcggccgcg cagccgaacg gcagttgacc
     2221 aaagtcttca aagcgaaaga gttcacgatt ctcatcgatc tggctcaagg caaggccggg
     2281 gcccacatgt ggacgacgga tttgtcttac gaatacgtgc gaatcaacgc gagttatcgt
     2341 tcgtaaggag cctcgcgcgg ttgcaaaatc gcggccactg tgctagcgtt cgggcgtttc
     2401 gcgcaatgaa cggtttgtcc catcgttcgc cgcgcggcat tcaaaatcag ggacaactta
     2461 attaaatcca caccagcgtg atgcgcacgc tgcgcagcct gagaataagg gggacatccc
     2521 ccctcgccgt gggcttttgc caggcgctct gcaggcttcg gagggaggtg aaggtatggg
     2581 agtagttgcg attaaggaat tactggaagc cggcgtccat ttcggacacc agaccaaccg
     2641 ttggaatccg aaaatgaagc ggtttctgtt cggagagcgc aacggcgtct acattattga
     2701 ccttcaacaa acggtcgaac gcatggagca ggcctatgcc ttcgctcgcg acaccgtggc
     2761 agccggtgac tccgtcctgt tcgtcggcac aaaacgccag gcggccgaaa ttttggaaga
     2821 agaagccaag cgcgccaatc agttctacat caaccagcgc tggctgggcg gcatgctgac
     2881 aaacttccag accatccgcc gcagcatcga caaaatgaag aagatggaag caaccctcgc
     2941 tgatccgacg catcagggcc atacgaaaaa agagctcgga cagatgcaga aggaagtcgt
     3001 caaactgcag aagaacttgt cgggtattcg caacatgcgg gctctgcccg gcgccgtgtt
     3061 cattctggat acgcgcatcg aacacattgc catcctcgag gccagccggc tggagattcc
     3121 cgtgattgcg atcgtcgatt cgaactgcga tccggatcac atccaatatc cgattcccgg
     3181 caacgacgac gcgattcgtt ccattaagct gattatctcc cggattgcgg acgcctgcct
     3241 tgaaggcgcc catctgcgcg cccagcagga agagacggag ttcgcatcgg caccggccgg
     3301 cggcgcggcc aaaccggcgc ccaaattggc cggcgtgtcc gcgtcctaag ggctgcatct
     3361 cgcacgatga cattgttcta ttcgattaac tgaggaagcc atggcaagtt tgagtgagct
     3421 ggtaaaagag ttgcgtgaaa agaccggggc cggcattctg gattgccaga aagccctaac
     3481 tgaaaacgga aacagcatcg ataaggccat cgactacctt cgacagaagg ggttggcggc
     3541 agcgcagaaa aaggccggac gggaaaccaa ccagggtctg attcacgcgt acattcatgc
     3601 cggaggaaaa atcggcgtgc tcatcgaagt gaactgcgaa accgatttcg tggcgcgcaa
     3661 tgagcagttc aaggccttcg tcaacgacct cgccctgcag gtagccgctg caagcccgtc
     3721 ctatgtgcga cgggaagaga tcgcagccga tgtggtggcc aaggaacgga gcatttacga
     3781 agggcaagcg aaggagctcg ggaagccgcc ggccgcctgg ccgaagatcg tcgaaggcaa
     3841 actcgaaaag ttcttccaag agaattgcct gctcgaacag gcgttcatca aggatccctc
     3901 cgtcgtcatc aaggatctcc tggcgcaaca aattgccacg atcggcgaga acatgaacat
     3961 ccgccggttt acacgcttcc aactaggcca agcatgagtt ctgctcacta ccgccgcatc
     4021 ctgctgaaag tcagcggaga aatgctggcc ggtgagcagg gctatggcat ccaaccctcg
     4081 attctcgaag ggctggcaga agaaattgcc gacgtcgtcg cgatggatgt cgaagtcgcc
     4141 gtcgttatcg gcggcggcaa tatcttccgc ggcctcgccg ccagcgcccg cggtatggag
     4201 cgggcatccg ccgattacat gggcatgctc gcgaccgtgc tgaatgcgct tgcgcttcaa
     4261 aacgcgctgg aaggtcgagg cgtcagtacc cgtgtccagt ccgccatcga gatgcgtcag
     4321 ttggccgaag gctacattcg ccggcgcgcc atccgccatt tggaaaagaa acgtgtcgtg
     4381 atttttgccg gaggaacggg caacccctat ttctccaccg acactgccgc gtcgctcagg
     4441 gcgatggaaa tcggcgcaca ggtcatcatg aaaggcacca aggtcgacgg catttacgac
     4501 agcgatccgg tcaaaaatcc gcaagccaaa aagtacaatg agattccctt cctctcgatt
     4561 ctgacccaga atctcaaagt catggattcg accgccatca gcctgtgcat ggacaaccga
     4621 ttgccgttga tcgtcttcaa tttgaaagaa aagggcaact tcaaacgtgt cgtccagggt
     4681 gatccgatcg gcaccctggt caccgtggaa agccgctaag ggagagactg atgtcgacgg
     4741 cgcaggaagt caaacagcgt gtgaccgaaa aaatggacca tgcgatcgag catctgaaac
     4801 gcgacctggc cggaatccgc accggtcgcg cctcagtcgc actgcttgat ggcatcaaag
     4861 tcgactatta cggcacgctg acgcccttga aacaggtggc caacgtggcc acgccggaag
     4921 cccggctcat cacgattcag ccgtgggaac agaacctgat ccgggaaatc gaaaaggcga
     4981 ttcaaatgtc cgatctcgga ctgacgccat cgaacgacgg caagatgatt cgcatccccc
     5041 ttccgccgct gaccgaagag cgccgcaagg atctcatcaa ggtctgcaag aaacacggcg
     5101 aagaaatgaa ggtgcaaatt cgtggcttcc gtcgagacgg caatgaagaa ctgaagaagc
     5161 tgcagaaaga cgccaagctc accgaagatg agctccgaaa gtcggaaaca gagattcaaa
     5221 agttcaccga ccagtatgtg cagaagatcg acgacatgat gaagaaaaaa gaaggcgaaa
     5281 ttctggaagt ctagcgccca atcctgttgt cagacactgc gtgtaccagc cgtccgaatc
     5341 ctccccaacc tccgcctccg tcgatctgac cgccctcgct cagaatgtcg cccacgtgcg
     5401 gcgactcgct ccccgtgctg atgtactggc cgtggtcaag gccaacgcgt acggacatgg
     5461 cgcggtcgag attacccgtg ccttgcaaca actggccgtg agacggttcg gcgtggccac
     5521 ggtagatgaa ggcgtcgcac tccgccaggc cggcatccgt gatgacattg tcgtcctagg
     5581 cgcgaccatg ccggctcaat ttccggatct cgtcgctcac cggctgactc ccgtcctcta
     5641 tcgtgccgac ttgctcccag cctttgcggc cacggtcaag accggtactg caccctaccc
     5701 cgtccacatt aaagtcgaaa ccggaatggg acgcctgggt gtgctcccgc gcgaactggg
     5761 cgaacttgtt tccagccctg aactacgcgg ccctctgcaa cttgagggcc tcatgaccca
     5821 tctcgctgac gcagacaatc cggatccgga acagacagaa gaacaactcg cgcgctttca
     5881 gcaggtgctc ccccaggtac aggaacgcgg cttcgcgatt ccgctgattc acgtggcgaa
     5941 cagcgcgggc atcatcaaat atcccaagag cctgcactcg ctggtgcgcc ccggcatcat
     6001 gctctatggc taccataccc tcgcgaacga caggcagggt cctgaattgc aaccgatcct
     6061 tacgtggcag accaccatcg cgcacatgca cacgattcca ccgggcagca gcgtgagcta
     6121 caaccgaacc ttcatcgcgt cgcgacagac gcgcgtagcg gtgctaccgg tcgggtatgc
     6181 cgacgggtac aaccggttgc tctcgaatcg tggacaggtg ttgatcggag gacaacgcgt
     6241 gccggtgatc ggacgagtct gcatggatat gacgatggtc gacgtcacgg acgtgcaagg
     6301 cgtccaagtt ggacaggagg caattctgat cggacaacag ggcggtgaac ggatcaccgc
     6361 agccgacctc gccgcctggc aacagaccat tccctacgag attctctgcg ccattggtcc
     6421 ccgcgtgccg cgccgctatc gcccactcat ggcttcgacc gatagcgcgt cccgaaccag
     6481 ctgacacctt cgagactcag cacaggtctt taccgaacgg cggcgcgaca tccgcttgcc
     6541 ttccctgccc cggttccctc atagtcgata cgtagccgca cacgcgcagt ccgatcacaa
     6601 tccatctcac atgtgttctg aggacatgaa catgatctgc tggatcccgt tgacgcagca
     6661 acacctgatg caatggttcg ccctcgtcct gcttgccgcc ccggtcacat cagcgatagc
     6721 cgcgggaacc caggcatcgg cgcccgatgg aacaaaaccg tccgccatga ccatccacat
     6781 tccgattccg gtcacaccgc ttgcagagtt gtatgggaac gtcaatcagc aactggccgc
     6841 ccatcgaagc cgcggtcgtg aatgggtcac attgggtggt gggagcggct ttctcaagta
     6901 tcggctctgg ccagacgaac agggttcgac caccgcagga gaccgactat tgtcacagag
     6961 caccgtgcca ttcggcgtgg aatatgccaa gcagatcaaa gggtcgatca ccaaaatcgc
     7021 cgagtgcgga caacgcgatg cctccacggg aacgggtcgg ctttcggtca cggtggcaac
     7081 gatgttcaag cagggtcata gctatacggt ggtccctgcg agtcaggtca gcgcggtgca
     7141 gtctgctcaa tcctgtgtct tgtccgaaca aggcgtggac gcaagcccgt tgatggtgca
     7201 agtctatcga agcgacttga tggaagtgct gcccgcaatc gatcggaaag cggccagcct
     7261 cgtccccatt aaaccggcag tcgcccgaat ctggaacgat cttcaagagc ccatgctgtt
     7321 ggatgagaca gaacaattgt ggctcatgct gaaccctgat accaccgccg cggccggagt
     7381 cgcaccgcta tcaggaagtc ccgcagccgg gtatggtgtg atcgcacggc ccacggtcgt
     7441 acgcggcacc aaacccgcgt cgcgccatct gcccctgcct gagccacagg atcacttccg
     7501 tgacaacggg tttcacgtcg attttgacct gcaagtgccg atcgaagaag cgaaccagcg
     7561 gctgcgtgag gccgtcatcg gtcaggaatg gtcgctgggc gtggggacga tcaagatcgt
     7621 caacgcgacg ctctatccat tgggccatca ggtgggcgtc gagttggtcc tgcgagggtt
     7681 attaccgctg accctgcgcc tcaaggggac accggcctac gatgaatccg cagggcgtat
     7741 tctgttccgg gaggtcgatt acaccatcaa ggaacgcacc cccgccacgg acctggccga
     7801 agaatggctc catgaaccgc tgcgggaaga attggccggc aggctgtcgc tgccgattcg
     7861 cgaggaatta gacctcatgc ggcaggcact cgaaaagggg ctcaaccgcg agctgaccgg
     7921 tggacggttg cgcgggacgg tgcggcagtt gtcgctggaa gacctggcgg tgcagaccgc
     7981 cagtctctcg gtccgcttca agaccgaagg cacgctgcgc tatgacgccc gtcccgacgt
     8041 caccgcgcca tgagcgtgat tagatcttca gcttccgctt cagctgtttt tcgacactgg
     8101 acactttgcc tgagagccgt cccttcggtc ccttgcgata gtcgatcttg atgctacagg
     8161 atacgcgatt acagtccttg cgcatgcgca tgtagcattt cttgacgacc gccatcacct
     8221 cgtcccactc cccttccagc accgtgccca tcggattcaa tcgatagtcc actccgctct
     8281 tgtcgataat gtccagcgag cgcgcgacat acttactgac actttcgccc ttgcccagcg
     8341 gcgacatgct gaattccaat aacaccatgg tccagttgtc cttcctatgt cgtcgatgcc
     8401 ggggccgcgg gcgcctgctg actggcgcgc tgctcctgcc agaccttcgg gtattgcacc
     8461 ttgcccagga gacggaatcc atccagcgct tcacgcagga gtgcgcggcg cttctcggca
     8521 tccggctcgt tggcctttgc ctgctcgcgt agctgggcga gccaatcgac aaaagcggca
     8581 aattcgctat ccagtatccg ttccagatct tctttcatga atcccgataa ggccggcgcc
     8641 tggccgctgg agctgatcgc cacacgcacg tggccggagc tgacgacagc aggcatgacg
     8701 acgttcgaaa catcgggctg gtcgacggac catagcagga atttctgttc gcgagccagc
     8761 ctgatcaacg aatgcgcgag ttcgcgatca tcgcgtaagg tgttcaagac cagaatgacg
     8821 ctctccagat cgttggcgcg aaagtgtcgt ccgcgatgaa tgattttcgc agaggccgcg
     8881 agcttgcgaa gcatttcgtg gagcgtgggg ctgacgaccg tcaccttggc gccggcatcc
     8941 aacagccgct gcaccttctc agccgcttcc tcattgccgc caatgacgag gaccggccac
     9001 ccgcgcacat cgagggaaat ttgaaacccg gaatttgcag ccatgattga tgttcctcca
     9061 acaagccgag aataacgtga acgcgtatca tacaacaggg catttcgcag ggtaaaccgt
     9121 ccgcgccatt cccctttacg ctatgagctg ggtataatgc gcccgcagtt caagacggtc
     9181 gctttcagag ctcttgcgcc aacaaaggag tgtattgtgg ctggatatac aggacggtcc
     9241 ctagcgatgg ccggcggaat catcggcctg gcggccgtgg cagcctattt cggtgtggcc
     9301 cacatcgggg agccggatca aggcggagga tcgatcgcag gacaggtaca aattgctccc
     9361 aatctggccg atcaggtcaa accgacggac gtgctgtttg tcatcgtgcg ccgaccaacc
     9421 ggtcctccgc gacctatcgc ggccaaacga ttcgacggac cgaagtttcc cgtccagttc
     9481 gaaattacca acgacgatgt gatggtaaag ggaaatgagc tgaaagggat ggtcgatgtg
     9541 atcgcccgcc tggatcgcga tggccaagcc ggccaaccgc aaccgggcga tatggaagga
     9601 cggtacccga agaatccgac cctgcccggg ggccgcgatc tcatgattac gatcgataag
     9661 gtctattaac ggctaaggac gctaggtgtc cggagaatcg gcgtccagct cgagattcca
     9721 atagagataa tcacgccagc tttccggggt gttgcgaatc ccgatggtga tggtgaccgt
     9781 ggggctccag cgaggcttga cgggtttctt gatcagacgc atgttggctt cttccggcgt
     9841 cctcccgctc ttccggttgt tgcatcgcca acaggccgtc acgatgtttt cccaggtctt
     9901 ccggccgcct ttcgcaatgg gcacgacgtg atcgaacgtg agttcttccg tgcggaactt
     9961 gtgtttgcag tattggcagc agtagccgtc gcgggtgaaa atgttgatac gggaaaactt
    10021 cacggcgcga tgactgtcct tgagcttgac cagcttcagg agccgcatga ccgaggggag
    10081 tttgatcgag atcgagatgc cgtggatttg acggtcgtag acttcgagga cttcgacctt
    10141 cccctgccag agcaaggtga tggccttttg ccaatgcagg acacgcagcg gttcataggt
    10201 ggagttgagc aacaaggtca tttccatagt ggctccggcg gtctcctatg agggcactat
    10261 ttacagcata gcaaatgacg gcgctggcct gacccttccg tgaatggttc ccgcttcatc
    10321 tatgccatat gccactaatg aaatcaagcg cttaggctct agcttgcaga atgttactcg
    10381 gccgtaaatc aatctggatc attctgtaac gcgtacaagg aacgcatgaa ttcttacgac
    10441 ggttatgtca ccgtggccca ggccgatgac attccaccag gcgcgtgtcg taccgtggag
    10501 gtcgacggca tcttcctcgc gctctccaat gtcgatggaa cgtttcgcgc cgtggacaat
    10561 acgtgcccgc acgcaggtgg tccgctgggc gaagggtgcc tggatggtga actcatcgaa
    10621 tgcccctggc acggctggcg attcaatgtg cgaaccggcg agcggccgga gaatccggag
    10681 atccgcgtca cctgtgtaga ggtgcgtgta ctggggacca acgtgcaggt gaaagtgccg
    10741 cttacattgt aggagggaac ggaggcgcac cggcgggagg agttcctgcg cgtccgctat
    10801 ccggaccttc aggcatcaaa ccatcggccg gcagggaggc cgcattcaca cgcttcgtgc
    10861 caagatccac gtgccagata aaatcacgct cgaaccactg cgtcgtcccc tgctcgcgca
    10921 tgtaaattcg aacttcgtat cgctgctcat cctgcttgat cacttgccat tcccctaatc
    10981 gcagtcccaa tttgcggtcc gctaaaattc gcgctcgttc gttgaacgcc tgaaggattg
    11041 ttggatagcc ggccgcgcga tgcgtctgca ccagcgccaa cgcctcggcc gcgcgcggat
    11101 cggtgatggg gttgatgccc ggaggcttca tccaccaata gaccagcccc ccgacgacga
    11161 gaagcaggag caccgcgtaa tgaatgactt tactcataag ctgtaacgat caccgtcgat
    11221 gcatgatgaa cgaactgatc actataggtg gcaggtgata atactgtcaa tcggtcacga
    11281 actcgcggga cggccatcga ctgagacgcg ccgcactctt accgcgcttg acaaggccgc
    11341 gcagcgctgt ggtacaagta ccggtcaacc tacaagacac catgggagcc tcaccgttca
    11401 atgaagttct tcttttacgc ggacaatttg aacccgtccc aactcaagcg tcgtgccccg
    11461 gaacataagt ttctcttcac ggcctatctg cctgaccata gcgtgcaatt ctgccgctgg
    11521 tcgacgcagt ggcgctgcgg cctggccagc gtcgtcccgt cgccgggaga gaaagtctgg
    11581 ggagccgtct ttgaaatcac cgatgaagat ctcaagatcc tcgacgagtt cgaggacgat
    11641 gtcccgcagg gcgcgtaccg gcatctgccg gtcacggtga tcacggaaga cggggaaaag
    11701 ctcctggtca cgacccatgc ggccaatccc atcggcaagt tcaaaccgaa ggaacattat
    11761 atcgactggg tgctcaaggg catcaaacaa tggaagttgc ccgaagaatg cagcgagcag
    11821 tggaaagcgt ataagccagc ctgatagatc gccacagatc gtcacttacc catatattga
    11881 ggtcacgatg ccaaagccaa aacatcatat ccttgtctgc accaatgccc gaccgccggg
    11941 ccaccccaaa ccgtcctgcg gcggccaggg ttccgcgcaa ctgctcatga acttcaacat
    12001 gggcctgatg caacgcggca tcatgccggg cgaagtgctg gtcaccgggt cttcctgcct
    12061 gggcccttgc gagcagggac cgaccgtagt cgtctatccc gaaggcacct ggtattccaa
    12121 agtcacggaa gccgatgtcg cgaccatcct tgacgaacac atcaagggcg ggaagccggc
    12181 agaaaaattg aatcccgata gcatctggaa gtagctcgga tcatcctcct cgttgaggat
    12241 gatcaacatg cctttccagc aaggccgcag gcgactcaca cctggagggt atcctcaggg
    12301 gtacgttgag gagcgtgaag agctgagaac gacgctggaa ggcatgttcg tcattcaatt
    12361 gctatgagca cccctagcca caaagaacac aaacaccacg cgccccgctc tgtcggttgc
    12421 atgattatca cctgcagcga tacccgcaca cccgacaccg acacgagtgg ccagttgatc
    12481 cagaagcttc tgaaggagaa cgggcacacg atcgccgcct attatctcgt gaaggatgag
    12541 ccggcgcaga tcaaggcgcg gatcaccgaa ggggtagcaa acgactcggt ccaagccatc
    12601 atcattaatg gcggaaccgg aatctcccgg cgagattcga cgtttgaagc cgtggatgcg
    12661 atgctggaaa aacgtctgga tggattcggt gaagtctttc ggtttttgac ctatcaggac
    12721 atcggctcac cggccatcat gagccgggcc accgccggca tcatcaacgg ccgcgtgctc
    12781 ttctccactc ccggctcgga aaacgctgtg cgcctggcga tggaaaaact catcctcccc
    12841 gaactcggtc acctggtcaa agaactcacg aaatagcacg tcgtcagcga tcagctctca
    12901 gcaatcactc ttcagctttg aggtttgctc agaactgact gctgtgacct gatgtcactc
    12961 agtgtgatga acccagaagt aagtaggaag ccggttttcc cgcggcctca tgcaggatgc
    13021 tcaaaacggt catccggcga ggccacaagg agtgcggcga ctgaggcgta cccgcaggag
    13081 tgcttgcagg aatttcatga aacgagaagg aagccggaag ccgcttgctc cctctgcccg
    13141 acccaggata ctcaaaacgg tcattcagcg aggccgcagg cgaagcaaaa ccggaggcct
    13201 acccgtaagt tacgtcaagg attttgatga gacgagaacg ctgctggaag ccatgtcgaa
    13261 cgagggctga ggacgttcaa aatggccgtc cagcaaggcc gtaggaagcg cggcgactga
    13321 ggcgtaccct ggcggtacgt cgcagggagc ggtgcgaccg agaacgaagt tggagccagc
    13381 cttcagcatc ccattgcaga aggatgttca aaacggctgt ccagcaaggc cgcaggcgaa
    13441 gcaaaaccgg aggcgtaccc tcaggggtac gttgaggatt ttgatgagcc gagaacgctg
    13501 ctggaagccg ttttcaacat cctctagtct tggccgatct tgggttcaga atacacctgc
    13561 ttatccgcca gcctcgattg cgcgtaccgc ttgtagtgca tcaacagatc gcggaccgac
    13621 acgacgccga cgattgaccc gcccttggtc acggccaggt ggcgcacgcc gagatctccc
    13681 atcatatcct gggcatcatc gacggcctga ttgctctcga tggtgcacag cggggaggtc
    13741 atcacctgtt cgacggtcaa cttgcccaca tcctgattgg tggccaaacc tttccgaacc
    13801 agatccgtgt cggtcacgat cccgacatga ttcttcccct tcttgatcaa gagggagctg
    13861 acccgcaggg tgcgcatctt cttcgccgca ccacgaaggg acatcgtcgg cccgacgctt
    13921 tgaggtttct tgttcataat ttgcgcaaca gtcgccatgt cacatacctt tctgcccgtt
    13981 gggacaagag agccgataat gaagccgatt acgaaacagt cagcacgatt ttaccaaaaa
    14041 acttccgatt caaaagatat tcttgcgcac ctttggcctc ggagagcgga aaggttcgat
    14101 caatcaccgg ccgcaacttg ccttcgccaa cgagatgagc ggctttcaac aactcactct
    14161 gcgaccccat gtacgagccc ttgatcgtga gctggcgcga atacacgtag cgtaagtcga
    14221 gcttgacctc cgcgcccgtc gtcgcaccgc aggtgacgag ccgtccacct ttcgccaatg
    14281 aatcgatgca ctgcgtccac acttccggac caatgtgctc aatcaccaca tccacaccac
    14341 ggcgctccgt cagttcccgc acccgctgat tgacctgctc acggctgtgg atgatgactt
    14401 cgtccgcccc cagatccttc gccttcgcga ccttctcctc gcttccgacg gtggtcaaga
    14461 cacgagcgcc ggcgagcttg gccatttgaa tcgccatgtg ccccaccccg ctgcccgcgc
    14521 ccataatcaa cacctcctcg ccgggctgca cctccgccaa tccgaacaac atatgccagg
    14581 cggtgacgga gaccagagga aaggccgcag cctgctcgaa agacagcgcg ccgggaatcg
    14641 gtcgcacatt ccgggccggc acctttacat agtctgcgta gccgccatgg cacatcgccc
    14701 cgatcaaacc ataagagcgg cacatattgt cacgaccggc cagacattgt tcgcacttcc
    14761 cgcatccgac accgggagac acatagatgc gctcgccaac cgcaaccccc tccacatgtg
    14821 agccgatctg gtgcaccacg ccggcgatgt ccgacccgag gatatgcggg agcggcatcg
    14881 gataggccgg actcccctgc ctgatccaga tatccagatg gttcaacgcg caggccttca
    14941 cctgcaccag gacttcgtcg acgccgatcg tcggcatcgg catctcctga tactggagct
    15001 ttcccggccc gccatgttcg tgaaacacca ccgcgttcat atcatcctca cctgtcattc
    15061 atgactacat gacgtttcat gccgcactgt gattccgaga gtacaggtcc ttaaccatct
    15121 cccgcaggac gatcaaaacg ccctgtcaca aggccgcagg gagcgcggcg actgagtcgt
    15181 acccttgtgg tacgtcgcaa ggagacgtac gaccgggaac gcagtgagat ggcgttttca
    15241 tcatcctgct agaaagtgag cacgccctcc acgaccatta ccgcttctcc tccaaccatc
    15301 acaccctgaa tcgcgtcgtc gtcggagttc acctggatca aaatgcgtga cgggcggtcg
    15361 atctcgtacc cctgttcggc ggtgatctcg gtttccggcc tgatatccac gactccgtgc
    15421 tgcacgagat aggcacccag tgcgccactg gcgctgccgg tcgccggatc ttccacaatg
    15481 ccgatcaacg gcgcaaacat gcgagtgtgc acggtggagg attgctcgac agtcatcgtg
    15541 ctgaagacca tcatgccgtt ggcgccatac tgttgagaca attcggcgat ggccgcaaca
    15601 tctggcacga tcgaacgcac cgccgtgagc gtgcgtaccg ggacgatgat gaccggcaac
    15661 cctgtcgaca ctacctgcac tggaaagcgg ctttcggtaa tggccgcttt ggtgatgccc
    15721 agagcgcggg cgatggcgaa caacgcctcc ggttcctcga tcacatccaa aaactccggg
    15781 ctcggttgcg ccatgaccac ccgttcgatc acgccgtcat gcgagtggat atcgacgggg
    15841 aacagcccca gattacattc ctggagcacc cgtgtgaccg gctcccgaag cgcgaggcga
    15901 tccaatgtgc ccaacacgaa aaaagtcccg atgacggggt ggccggcgaa gggaatttcc
    15961 tgcgtcggcg tgaaaatgcg catcttgacg acagccgcct ggtcggtggg aggaaacacg
    16021 aacaccgttt cggagagatt catctcgcgg gcgatctgct gcaattcgac gtcggtcaag
    16081 ccatcggcat ccggaaacac cgccaccgga ttcccgccga agggttcatc ggtaaacacg
    16141 tcggcttgat aaaactgcaa tcgccgttgc ccgggcatac actcctcaaa aagatgctgg
    16201 cggcatgttg ccccgagtca gtattttttg caagtgggca agaacgcgac actggccggt
    16261 gtgtaggaat tcaaacggct gctcaaaacg gcatctggca aggccgcagg gagcgtggcg
    16321 actgaagcgt acccgtgcgg tacgttgcag ggaggcacgt gaccgagtac gccgccaaat
    16381 ggcgttttca tctcctggaa ttcgcaattc gcaggctgct caaaacggcg tctcacgagg
    16441 ccgcaggcga gttaaaaccg gaagcgtacc ctcaggggta tcttgagggt ttcggcggga
    16501 tggcgcatgt acacaaatct acagaccggc tggtcaaaac gccatgtggc aaggccgcag
    16561 ggagcgtggc gactgaagcg tacccgtgcg gtacgttgca gggaggcaca tgaccgagaa
    16621 cgccgccaga tggcgttttc accagccggc taagcggggc gtggaatggc ctcgtacgcc
    16681 gccctcatcg gatggttgag gtattgctcg acatagttct gtagcgaacc gtgccggtac
    16741 agctgctggt tggcaaagcg agtgtcgatc ttgtgcaaca ccttcacgcg cacgccggtc
    16801 tttttggtga actgctccag actcacgccg gtgatgtccg tataaggtcc ccgtcccgac
    16861 tgcataatgc atttcggcac gtggatcgcc ttttccttcg tcagtcgccg cgcgatatca
    16921 tcgaaggtga ggagtacggt gcaatcggaa tcaccgctga gcagggcatt gggcacgtac
    16981 agaaacttcg cgcgattctt ccggaacatg gtcaggatct tgtggacact ccccgccatc
    17041 accaccgtat cctttttttc caattccaac gaatcgaaca ggctgacgat gcggcggcgg
    17101 ctcaagaatg ccgtggtgta ggactcggtg tgaatggtct gcagattcgg caggcccaag
    17161 tgatagatgc gcgtcgcttc atcaggcagg aactttcgcc cctgccgcat gagcgaggcc
    17221 gtctcttctt tcagtccacg aaccggcgtg agcgtgccca tccacagcac gcactgctgg
    17281 ttgatcgcgg aaatcgtcgc ggcatccttc gacatcgtgt gctgatcgaa cgcgtagaag
    17341 ttggctgacg acacggcggg tccatccagc accttcataa gatgtttgac ggacggcgca
    17401 tgcggcatca gccgctgccg gtagtcgctg agcgtgatga ccgagagctc gaagttgatc
    17461 cggcccggat actgcgccac cagttgatgg atcttcggca catgcacaaa gccgacagta
    17521 aaaaactgaa tgctcttgtc ggtgtatttc agaaaatctt cgacccattc cattgccttg
    17581 gggtgcagga acagatcggt ccattccatg aactcgttcc caccgaggca ccagaactgc
    17641 gtagggtcgg tcggtttccg gctgatgtac tcgaggatga acttccaatc ctcatcactg
    17701 gtcttggggg gatcgagcgt ttcgcggtag gagtggtcga gttcgtaaca gaactcacat
    17761 ttaaccggac agtcgcgccc gaggctcagg ggaattttcc ctgcgtccat ttctctgacg
    17821 agcacttgcc gatagtcggt gcgatctttg aagatcgggg tcggctgaaa aatcgggaga
    17881 gtggtgggca tgggtaaacc tgtttgccta tcgccgttgg ggcaatctgt gtgagattga
    17941 cagggtcacc atcgacttgc cagactccgt gacatggctg agaaacgttt caccgaggct
    18001 ctcgcactgt tcgcactcac cgagcccttc acccgagaac aactcgatca gaagcgcgat
    18061 gccctgctcc acacctggac cccgcgccgc ttcgccaacc tgaccaacaa ccccaagaaa
    18121 tacatgcagg cctatacgaa agccgaagag atgacccgcc tgatcgaaac ctcgtacgcc
    18181 ctactcgctg cgatcgtcgt tcccgacgag gcgaggaggg ccccgcgtgg gacgtaaaac
    18241 gtgaacaggt gaaacggtct aacgtttcac gcctgacgcc ctccatcttc agcatcccct
    18301 accggatgat caaaacgtca tgtcacaagg ccgcaggcga gttaaaaccg gaggcgtacc
    18361 cccaggggta cgttgaggat tttaactagc cgagaacgcc gtgagatggc gttttcatca
    18421 tccgacctct cggttgtcgt tttcggcagc ctgctattca acgccgtggg aaaccacgcg
    18481 cgacatcgac gtctccccgt ccttctctcc tgccccctcg gccacggcca tcggccaggt
    18541 gacgagaccc gacacacctt ccgtcgcggc gccgtccttc gtggccgcat aaatctgcgg
    18601 caacttgttc gtgccgaact tggagatgta caggaaaacg tgttcgcggc tgttgacccg
    18661 caccgcccag ggctgaaagt cgttcttata aaactcttcg cagagctgca tcgcgtcgag
    18721 acaggaaatg cccaccggct cgttcagcgt gtaatagtaa tccagcggca ggtcgtactc
    18781 ctcctgctta tggatcgtga tgccgaattt gtccggattg cgcaccatcg gcgtgtggcg
    18841 gtaggccacg aaataaaaca gctcgaccga atgaatcacc ggctgattgt cgatgacgaa
    18901 ctgcctcgtc tccagcgcct cgtcacgcgt ctcgccgggg aacccgtaga acgccatcac
    18961 gtggttccag atccccgcct tggctgcctg atgcagattg ttctggatca cactcttccg
    19021 cgcatgcttg tccatgagat tcaggacgcg ctcgttcgcc gactccatgc cgtagtacaa
    19081 cgtgcagcag ccggccttcg ccgccagatc ccaaatcgcc tggtcctgca aggtctcctc
    19141 gaagcggatc aacgtcgtcc acttgatgcc gacgttccga tccacgagca attgcgacac
    19201 cttcttgaac aacgcgggcg gataggactc gtctgaaaac aggaagtgac ggcagttgta
    19261 cttatcccgc agtgccgtga cctgctcgac cacatgctgc gcgctcatgc cgcggtactg
    19321 gtcgaaatag ccctggccgt gatcgcagaa ggtgcaccgg ccccagtagc agccgcgggt
    19381 ggcaagatag ggaatgatcc gctcgggcac gaaataatta tccagcggca ggccgtcgaa
    19441 gtccggcaac ggcagcgccg acgttttctc cgtgtacact tccttgctct gcttcacgcc
    19501 gtccgcgtcg cgatacatga gattgggcac ctggtctatc gcccgctggc cgttcacggc
    19561 ctccaggtac cagagcagcg catgctcacc ttcatacaag atggcggcgt cgaacacctc
    19621 ggtaaagaac cgctcgtggt gcgcccattc ttcctgcaac cgcgtgacga cgttgccgcc
    19681 gaccacgacg cggatctccg gaaaactctc tttgatcatc ttgcagaacg taagaccggc
    19741 catcagctgc atttgcgtgc cgatggagat gccgatcacc gagggctttt ccttgctcac
    19801 gctgggcagc accagctgat tgcagatgtc gcgatagaca ttcacctgct catcgtcgag
    19861 acaggcgaac acttccttcg a
//
LOCUS       DAOQEW010000053        19743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_692_length_19743_cov_8.851432, whole genome shotgun sequence.
ACCESSION   DAOQEW010000053 DAOQEW010000000
VERSION     DAOQEW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19743
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_19743_cov_8.851432"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="PLS35_07855"
     CDS             <1..1071
                     /locus_tag="PLS35_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI18611.1"
                     /translation="QVILGGFLPLLKGLMINPENAELQRSELADPRAEVKISQTRQDL
                     FLAAASQFWDWVSAAKFVDVQRRALGVAEERLRQVEGRAKAGAVAPLDVVEAGQEVQR
                     RREISIAAQRAVEQEQYKMSMFLWENQTPTTPQLERVPDFPPPAAAPTADVVKADKLQ
                     AKSDRPEIREVDIEAKVNNIDLELAKNNLLPSLDLEAAPARAPEKFVLGLGYRFGAEL
                     RIPILQRRSRGEVMEAQAQADRLVLVQKYREQQVVVDVDNAVSAIERAKERVAAAAES
                     LRMAKTLEEGERFRFSLGATSVLFVNLRERNSVDSEMQLVRAKADYQKALALYQWATG
                     SWARSQPSAVPVNYRVGASLSK"
     gene            1204..2871
                     /locus_tag="PLS35_07860"
     CDS             1204..2871
                     /locus_tag="PLS35_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI18612.1"
                     /translation="MSQDHSDNQSNLFQAVVGHLGLLFRLERRILGLIVSYSIAIGLF
                     SLIVPLTVQELVNTFAFALQPITIVTLAAVMVAGLLFVGAFRALQYYAVEVLERRIFA
                     RVAIAMAQQLPHLRYQGFKPRFANYFVETILMQRAVSVLLVDLINVIVGGAVGMTILV
                     FYHPYFLLFNAILLVGFNVVFFLMSHGGLKADMDMSHAKYDTLHWFQEIAYNLLHFKS
                     TDSQALLIKKTDQLLDNYVGVRRTRFGILIRQYLGSLGWQAIVHSGLIATAGWLLGIG
                     QLTLGQFVAAEVVVSGILSSFDGVVKRMGHIYYFLTALTELSFLFSLPKDQDAATLSI
                     PLPDPTVHGIRVTCKDLTFAPAGGPPVFEHFNLEVTPGEKIGIYADTTMAKTALARVL
                     GGLESPTAGVIRYNGVDLRHLNLDSVNRFRGFILDSQLSLFEGTLEENILLGRDYIPY
                     SDVRWALRFTELEEEVDALPHGLKTHVRSAGKIFAPTHIVRILVARAILGRPQLLIFE
                     GILHNMHPAMRETILRRLCSKEEPWSVIFVSNDPNLTPHVDRRIMLN"
     gene            3014..4690
                     /locus_tag="PLS35_07865"
     CDS             3014..4690
                     /locus_tag="PLS35_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18613.1"
                     /translation="MNPIATESTITTPSAWTILSRLNLLVELERRILAIIASYAVVIG
                     LFALIVPLTVQELVNTFAFAIQPIMIVTLVAIMLGALLLMGAFRVLQGRAVEILVQRI
                     YTRLAVAFTEALPRFRENVFLPQHTNTFIEAELLPRALVAMLVDIINVFVSGMIGMTI
                     LIMYHPYFLGYNIFLITGFAFLLTFFGRGGLRITQRVSNLHYQTFHWLQDIGINRLHF
                     KSTDSLPLLLKKTDALVQAYVMARKTRSDILTGAQYKSTVIFQAIAHSSMIGLGGWLL
                     SLADITLGQFVAAEVIVGTLLLNLDIVARRMYAAIYVATSMQELSRLFEMPKEEVSGP
                     IAAWLPNPTLQGVRLTCKDVSFASPEGPMLFDHFNLEVLPGEKISVLTGTSKSKTSLA
                     LLLAGLYHPTSGVIRYNDIDLRDVSLNYVNRCRGLMLDSHPTLFDGTLEENVTLQRPS
                     IQFADLSWALRFVELEEEIDALPLGLETVVHGNGANLSRSQVLRILLARMIVTRPELL
                     IFNGSLHNIDPTVRLTLLRRLCAKEEPWSIVFVSNDPEVGQLVERRVILD"
     gene            4806..5582
                     /locus_tag="PLS35_07870"
     CDS             4806..5582
                     /locus_tag="PLS35_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HNI18614.1"
                     /translation="MSFASRFKLSFPSTNQLIISLLIAGLGWISGQALSRIDQDLRIM
                     YTEYTLGAADLAHISADVIRYRNTIIRSLEAENQAVFERITESLPAQRARIQHAVDRY
                     AAAGLRVSRSGRSEEKDIQAVRESLDQYFHVASKTVDLLSQEWKAGTPGEATELRRKA
                     EIHAADNGGPKVMQVSLALDRLLETVAEVAKDMRDEGTKTIRSTSYWIVGGSFFIALL
                     NLFLSRTTRAPLSASRPEESVRPDSLDLPGGGSNTALRAE"
     gene            complement(5596..6306)
                     /locus_tag="PLS35_07875"
     CDS             complement(5596..6306)
                     /locus_tag="PLS35_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNI18615.1"
                     /translation="MRINKFFTEQGLCSRREADRLVAEGRVTINGVVAKLGDQVSSQD
                     VVARDGTILQRGNRAVYLKYHKPVGVTTTTELHVTRNIISEINHRERIFPIGRLDKDS
                     SGLILLTNDGDIVNEILRVEHGHEREYRVEVDREFDDAFLSRMAEGVVILGERTRPCL
                     MARVGPRRFRIILTEGRNRQIRRMCQALGYRVVSLHRVRIMHITVEGLHAGQWMDLSE
                     EERRRLFQALGRPPESSL"
     gene            complement(6403..7335)
                     /gene="speB"
                     /locus_tag="PLS35_07880"
     CDS             complement(6403..7335)
                     /gene="speB"
                     /locus_tag="PLS35_07880"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNI18616.1"
                     /translation="MALPSGWLGQADNFLGIDEPWCHPDQAGVYVLPAPYEHTSSYIL
                     GSDRGPSAIIEASQQVELYDETLRYEPYREWGGVATANTLNLNGRVDGQAVQAIQDFV
                     QPHVGRGKFLVTLTGEHTGALGAIRAHAQHYSGMCVVQIDAHGDLRQAYQDNPYSHAS
                     VMARVVQDGLPLVQVGIRSISPEEIELIDKTPRIKTFFAASILDPSGPYEGRAARWIP
                     EVLASCPGPVYLTFDCDGLDASLVPALGTPEPGGLGWYDTLALVTALANGPGIVGMDI
                     SEIAPIEGFVAPQFSIARLIYRMLGRVRAGRRVH"
     gene            complement(7344..7919)
                     /locus_tag="PLS35_07885"
     CDS             complement(7344..7919)
                     /locus_tag="PLS35_07885"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HNI18617.1"
                     /translation="MVPTHMFLTRGVGVHKEKLAAFEQALRSAGVAYCNLVSVSSILP
                     PNCKILPRKRGEKLLNPGEITFCVMARSETNERNRLISASIGLAIPTDRDTYGYLSEH
                     HAYGETDEESGEYTEDLAAQMLATTQGIEFDPDVAWKEREQVFKMGGKIVRTLNITQS
                     AVGRPNRWTCVIALAVFIPTENIPKSLRNKA"
     gene            complement(8095..9525)
                     /locus_tag="PLS35_07890"
     CDS             complement(8095..9525)
                     /locus_tag="PLS35_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNI18618.1"
                     /translation="MENGRPFLIGGRWSQSTTVAPVRNPYTGETIAHVCQASPADVES
                     AIQSSVDGAAAMRRLSGYARSTLLANAAQALQARQEEFARTMTAEAGKPLTDARREVG
                     RAIQTLSIAGEEAKRIGGEVVPLDWSPGMESYWGVTRRFPIGPILGITPFNFPLNLVV
                     HKVAPALAAGNSILIKPAPQTPLTALLLGDLLLKAGVPPGGLNILPCDNTLAEQLVVD
                     PRFKLLSFTGSAPVGWMLKAKCGKKKVVLELGGNAAVIIEPDADLEYAAQRCVTGGFT
                     YAGQTCISVQRIFVHESIAESFTEQLVARVRALATGDPGDERTVVGPLIDAGAAQRIE
                     GWIDEAITQGARLLSGGSREGSVVRPTVLSQVTAGMNVSCREVFGPLVTITPYREFEA
                     ALRAVNESDYGLQAGIFTTNIGRIFQAFEQLEVGGVLANEIPTFRADHMPYGGIKDSG
                     IGREGLRYAIEDMTEPKLLVVNLRRP"
     gene            complement(9553..9831)
                     /locus_tag="PLS35_07895"
     CDS             complement(9553..9831)
                     /locus_tag="PLS35_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18619.1"
                     /translation="MGSKQAFWTVPAKDGEPDLWVCMSCLSEAFCRKVPMPACPTCHG
                     VSTYEAFTLAAVQDWGTEELIAKAVAAQQEEAALSAAASAAPPVESVQ"
     gene            complement(9911..10564)
                     /locus_tag="PLS35_07900"
     CDS             complement(9911..10564)
                     /locus_tag="PLS35_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNI18620.1"
                     /translation="MTPLTGWMIRFATPFLLLVLTTASALAASSPAVDNRMRGKADAP
                     ITLIEYSDFTCGYCLKFFKETWPKIHARYVETGKVRFLYKDYPRADQGPGVTAALAAR
                     CAGDQGTYWPMHDRLFAADGRLDVDSYFQHAKAIGLDQSKFRECLRDAPHMKAIFQDR
                     DEANAWGFHGTPGFILMRTTQQPTAQNPALAIPGAFPFAAFEEEIEKLLAASGEKAK"
     gene            complement(10561..>11001)
                     /locus_tag="PLS35_07905"
     CDS             complement(10561..>11001)
                     /locus_tag="PLS35_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNI18621.1"
                     /translation="SQIKEGGDVAVLRALHQTCPVFNPYLDLNHYAEEVLGSTRLGIF
                     TIGGGVPRNWAQQVAPYIEISNTRLGLNVEPPRFHYGVRICPEPDYWGGLSGCTYQEG
                     ISWGKFVPPAEGGRFAEVLSDATLVWPLLMVGLLERFAAKSVSA"
     assembly_gap    11002..11101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11102..11373)
                     /locus_tag="PLS35_07910"
     CDS             complement(<11102..11373)
                     /locus_tag="PLS35_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNI18622.1"
                     /translation="MAGREFHDGAKDGLEALEPLDPEKIHSFTELLEAMRKTAFGGRR
                     LGEAYETLAAMIEDPDCKVVLTLSGAMTIAKMGKIISTMIDRGMVQA"
     gene            complement(11589..12341)
                     /locus_tag="PLS35_07915"
     CDS             complement(11589..12341)
                     /locus_tag="PLS35_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNI18623.1"
                     /translation="MLDVIGLGAVAGLIPVYLGIFAALYLGKVLPRTWEGGLIGVANG
                     VLVYLFFDLMHEATEQTGARDPLSWLVFLGSLGVSFVGLVALESSQVFGARSGNRILS
                     LPYMIAVGMGLHNLGEGLAIGASYAGGEYTLSALLVAGFGLHNGTEGFGIVGAAGKTP
                     ISWRDVALLGLIAGLPTCVGTFLSGQGVSSYFSICFYALAAGSLLYVVLSLTVMSYTA
                     TRRVQVSAGMFTGIAIMYLTAMILTLVSGVRS"
     gene            12518..13489
                     /locus_tag="PLS35_07920"
     CDS             12518..13489
                     /locus_tag="PLS35_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNI18624.1"
                     /translation="MTYQQLMSLANGYAESKTLLVANELGVFTAIGTGGRSAEELAVS
                     CGTVREGMKLLLQSLTGLGLLRLKAGRYWNTPLGRTFLDGGSPLAITNLLWLLNHHWV
                     DWSGMTKAIRRGPPGWAPVTKTANFRRRFALAMQERSHILVQPTIDSFRLPRQAERFL
                     DLGGGAGSYAIALARRYPTLQGLVIDQSVTVAKRLIRRERLANRIQVQEGDLRTLPLP
                     TDLDVVLLANVLHDFNKTENRHLLRRTRKALRPGGRLFIVEYFLAREETRPAEAAVFS
                     LLMFAFTGTGRCYSWNEVEAWLRQAGFGRCRRHKVTGSIGTLEATKL"
     gene            complement(13509..14624)
                     /locus_tag="PLS35_07925"
     CDS             complement(13509..14624)
                     /locus_tag="PLS35_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18625.1"
                     /translation="MPTGLPPGLLRVIDQIVCVAGTSGRGTDLWDGIRVECEREYSGI
                     RHGKGTLAHALEWEILKLQTRLESSAYGNPVVPVPLFRNRHVHIGSLIRLWRRLALET
                     EEWLASQGYDTLLDVGPWGGFNFVLEPDGYTRMPFARLTLAVGGLPSTPFHEQGGPLF
                     QHLLPRYHAELQQAGVNFPPLWQYHHAKHDASVRLREVSATHYLPTHSYERRTFVKVR
                     VSRDCEAVEEIALQDFLLLLERLQFTTDWELYREQTKDVDARFDLQDFISLNHMVEGV
                     YQRTDAEDRLLHEMKDAFRGAIRARQVLYGYLSQVVKLRWVENLYWALAEAALGVKRY
                     QRMVSFDREVCPQIPPRLLIPVRRHLNRYHNRVGGTA"
     gene            complement(14742..14957)
                     /locus_tag="PLS35_07930"
     CDS             complement(14742..14957)
                     /locus_tag="PLS35_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18626.1"
                     /translation="MNRNDFLVALFGRPVWFHCKQCHRVMAGGTEESFPDMWQEEAVA
                     MEAWRVRPEDLWWFDTVCGSCQEQQRS"
     gene            complement(15313..15957)
                     /locus_tag="PLS35_07935"
     CDS             complement(15313..15957)
                     /locus_tag="PLS35_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18627.1"
                     /translation="MRIAQISQCFEEVSNESERPEVRGVEFVTRSLMRFGHDVTVFAS
                     GDSRVSGRLIPVSPQAIRHYPSPKRHLSEGLTFLAIEKAFSMESSFDLLHVHAGFVAF
                     PMMRRSPLPSVATVYGSLDAPEILQLFRAFRELPLVATSAEQIRQCPELNWLALIPVP
                     GPRQRSEVPAETMDSLVETATAYTAVYESIVERAVRPTHAVRPAYSLYDVQTTV"
     gene            complement(16202..16846)
                     /locus_tag="PLS35_07940"
     CDS             complement(16202..16846)
                     /locus_tag="PLS35_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18628.1"
                     /translation="MSKPRVLLADDHSLVLEGFRRILADQCELIGMVEDGRALLEAAA
                     RLEPDIVILDVSMPLLNGIDAATQLKKTHPSMKVIFVTMHSDADYVRSAFEAGASGYL
                     LKRSAVDELEQAIRAVWAGHSYITPLIAKDLLDVLLTAGPGQTPQKKTLTFRQREVLQ
                     LLAEGRTVKDIALRLKISTRTVEFHKAQVMEQLNLRTTAELIKYALTHGLLAPS"
     gene            complement(16839..18005)
                     /locus_tag="PLS35_07945"
     CDS             complement(16839..18005)
                     /locus_tag="PLS35_07945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNI18629.1"
                     /translation="MTQPSHRLLLLTIIILGIGLYTLDLYLPLGIGNGVLYGGLVVMS
                     LAIPDRKIPLLVAGTCSVFALSDVFLGVTLPNVPLWMGLSNRMFSLTAIWFPVVYAFQ
                     RRKSEEALRNAHNELEIRVEARTRELALVNQALVGEIEERMETERSLRASESSLKVSQ
                     EELRQSREELRALAGQLLTAQEVERRRIARDLHDDVNQRLAMLAMDLRRIEKGEAGDL
                     SNIGGMVKSISGRLTAVSDDVRQMAYRFHPSILDDLGLMKAVRRLVDDFSASTGIEAV
                     YVYQDCLSPVPTDLATCVYRIAQESLNNVARHAKATEVEVELICDEGMITLSVRDNGV
                     GFDAVHASQGRGRLGLLSMKERVRLVRGTLNVSSSPGGGTHIEVGVPLSGSQHV"
     gene            complement(18204..18716)
                     /locus_tag="PLS35_07950"
     CDS             complement(18204..18716)
                     /locus_tag="PLS35_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HNI18630.1"
                     /translation="MKKQGILVLAGLLIGLQSGTAFAVNPDNGPGCGLGKLAWSDYKT
                     PKNIAPQVMMATTNGTFGSQTFGISFGTSGCTNDGKVMASQETNVFVASTFDTLSTDM
                     ARGGGEHLTALATLMNVPVEQQPAFFQLTQQQYLALTEAGEVSSVAVVKALQEAMTGH
                     PTLAQLAGTR"
     gene            19150..>19743
                     /locus_tag="PLS35_07955"
     CDS             19150..>19743
                     /locus_tag="PLS35_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18631.1"
                     /translation="MPSTAPIPPHARPPRIASIESYRVVAILLIVSLHTNLIARLHLI
                     GGGVGFLVDMPLYLLFWISVPYFFLTAGYFYGRTVDAGAAPFSLLRRSCRSLTLLFVI
                     WVAVYSIIGPNWISNVYTQGLWSTISTEASHTMGRLMQEHVTLLLVPRPPIFHLWFLP
                     ALIAGVSTVAMVVTSRLKTWVGALLVSTYILLVVSAVV"
BASE COUNT         4167 a         6044 c         5434 g         3998 t
ORIGIN      
        1 caggtcattc tcgggggctt tctgcctctg ctgaaagggc tcatgatcaa ccccgagaac
       61 gcggaactgc agcgttcgga gctggctgat cctcgcgccg aagtcaaaat atcgcaaacc
      121 agacaggatc tctttctggc cgccgcgagc caattctggg actgggtctc cgccgccaag
      181 tttgtcgacg tgcaacgccg ggccctcggc gtcgcggagg aacggttgag acaggtggaa
      241 ggcagagcta aggctggagc cgtggcaccc ctcgacgtcg tcgaggcagg ccaggaagtg
      301 cagcgtcgcc gtgaaatctc catcgccgcg cagcgagccg tggagcagga acaatataaa
      361 atgtcgatgt tcctctggga aaaccagacg ccgacgaccc cgcagctcga gcgcgttccg
      421 gattttcccc ctcccgcagc ggccccgacg gcagacgtcg tcaaagcgga taaactccag
      481 gcgaaaagcg atcgcccgga aattcgcgaa gtcgatatcg aagccaaggt taataatatc
      541 gatctggaac tcgccaagaa caatctcctg ccgagtctcg atctggaagc ggcgccggcc
      601 agggctccgg agaaatttgt gctgggattg ggctatcggt tcggggccga gctgcgtatc
      661 ccgatccttc aacggagaag ccggggtgaa gtgatggagg cccaggcgca agccgatcgc
      721 ctggtgctgg tgcagaaata tcgggaacag caagtggtcg tggatgtcga caatgccgtg
      781 tcggccatcg aacgggcgaa ggaacgggtc gcggcggcgg ccgaatcact tcgcatggcc
      841 aagaccttgg aagaaggtga acgtttccgg ttcagcctgg gcgcaacgag cgtgctgttt
      901 gtgaacctgc gggagcggaa ttccgtcgat tcggagatgc aactcgtccg ggccaaggcg
      961 gattaccaga aggctctcgc cctctatcaa tgggcgacgg gatcttgggc caggagccag
     1021 ccctcagccg ttccggtcaa ctaccgggtc ggtgcgagct tgtccaaata gtagtatttt
     1081 acggtgcgct ctgataggtt ttagctggtg taggcctgcg cggacaggcc gccgcaacaa
     1141 tcatcatccg gccatcgccg caacacggcc gatgggttga tgaggcgcgg caggggtagc
     1201 aggatgtccc aggatcattc cgacaaccag agcaatttgt ttcaagcggt cgtcggccat
     1261 ctgggcctcc tgtttcgtct ggaacggcgc atcctcggtc tcatcgtttc ctattccatc
     1321 gccatcgggc ttttctcgct gatcgttcct ctcacagttc aggaactcgt caatacgttc
     1381 gccttcgcgc tccagcccat caccattgtc acactggccg cggtcatggt ggcgggcctg
     1441 ctcttcgtcg gcgccttccg ggccctccaa tattatgcgg tggaagtcct ggagcggcgc
     1501 atcttcgccc gggtggccat tgccatggcc caacaattgc cgcatttgcg gtatcagggc
     1561 ttcaagcctc gattcgccaa ttacttcgtc gaaaccattc tcatgcagcg ggccgtatcg
     1621 gtgctgctgg tcgacttgat caacgtcatc gtcggcgggg cggtcggtat gaccatcctc
     1681 gtgttctacc acccgtactt cctgttgttt aacgccatct tgctggtcgg gttcaacgtg
     1741 gtgttctttc tcatgagcca tggcgggctg aaggccgaca tggacatgtc ccacgccaaa
     1801 tacgacaccc tgcactggtt tcaggaaatt gcgtacaacc tgctccattt caagtccacc
     1861 gatagccagg cgctgctgat caagaagacg gatcaactcc tggacaacta tgtcggggtg
     1921 cgcagaaccc gcttcggcat tctcatccgg caatatctgg gatcactcgg atggcaggcg
     1981 atcgtccata gcggcctcat cgcaacggct ggttggctcc tgggaatcgg ccagctgacg
     2041 ctcggacaat tcgtcgccgc ggaagtcgtg gtcagcggca tcctctcaag cttcgacggc
     2101 gtcgtgaagc ggatggggca tatctattat tttctcaccg ccttgaccga attgagcttc
     2161 ctcttctcgt tgcccaaaga tcaggacgcc gccacgctgt ccattccctt gcccgacccg
     2221 acggtgcacg gcattcgagt cacgtgcaaa gacctcacgt ttgcgccagc cggcggcccg
     2281 cccgtatttg agcacttcaa cctggaagtg acgcccggtg aaaaaatcgg gatctatgcc
     2341 gatacgacga tggccaaaac cgcgctcgcc cgggtgctcg gcggactcga atcaccgaca
     2401 gccggcgtca tccgctacaa cggcgtcgat ctgcgccatc tcaacctgga ttccgtcaat
     2461 cgcttcaggg gattcatcct ggattcacaa ctctcgttgt ttgaggggac actggaagaa
     2521 aacattctgc tcgggcggga ctacattccc tatagcgatg tgcggtgggc cctccgcttc
     2581 accgaactgg aagaagaagt cgacgccctc ccccatggct tgaaaacaca tgtgcggtct
     2641 gcgggtaaaa tcttcgcccc tacgcacatc gtgcgcatcc tggtggcgcg agcgattcta
     2701 ggacgcccgc agctcctcat tttcgaaggc attcttcaca acatgcatcc cgcgatgcgc
     2761 gagacgattc tccgccgctt atgcagcaag gaagaaccct ggtcggtgat tttcgtctcg
     2821 aacgatccga atctcacccc gcatgtcgac cgccggatca tgctgaacta acggcctgcc
     2881 acaccgtcac ctaggctgct ggcctcgggc tctcgcttct ttcccacccg ccgaccaccg
     2941 tgtttcccgt tcttgacaga gatgcggcat tcgcccacac tgcgccccac catcggtcga
     3001 cacgagctat cgcgtgaacc ccatcgctac cgaatcaacg atcaccacac cttcagcctg
     3061 gacgattctt tcgcgtctca acctcctggt cgaactcgag cggcgcattc tggccatcat
     3121 tgcctcttat gcagtcgtga tcggtctgtt tgcgctgatc gtgccgctca ccgtgcaaga
     3181 gctcgtgaac accttcgcct tcgccattca accgatcatg atcgtgacgc tggtcgccat
     3241 catgttaggc gcgttgctgt tgatgggggc attccgggtc ttgcagggac gcgcggtgga
     3301 aatcctggtg cagcggatct atactaggct ggctgtcgcc tttaccgaag ccctgccccg
     3361 gttccgggag aacgtcttcc ttccgcaaca caccaacacc ttcatcgaag cagagctgtt
     3421 gccccgtgcg ctggtcgcga tgctggtcga catcatcaac gtgttcgtgt cgggcatgat
     3481 cggcatgacg atcctgatca tgtaccatcc ctacttcctc ggctataaca tcttcctgat
     3541 caccggattt gcttttcttc tgaccttttt tggccgcgga ggactccgga ttactcaacg
     3601 agtctccaac cttcactacc agacgtttca ctggttgcag gacatcggca tcaaccggct
     3661 ccatttcaaa tctaccgaca gcctgccgct tctgctcaag aagaccgatg cgctcgtgca
     3721 ggcctatgtc atggcgagga agacccgatc cgatattctg accggggcgc aatacaagag
     3781 tacggtcatc tttcaggcca tcgcccatag cagcatgatc ggcctcggcg gatggttact
     3841 ctccctcgcg gacattaccc tcggccaatt cgttgcggcg gaggtcattg tcggcaccct
     3901 gcttctcaat ctcgacatcg tcgcccgtcg catgtacgcc gctatctacg tcgccacatc
     3961 catgcaggag ctctccaggc tgttcgaaat gccgaaagag gaggtctccg gtcccatcgc
     4021 cgcctggctg ccgaatccca ctctgcaggg agtacgactg acgtgcaaag acgtgtcctt
     4081 tgccagccct gagggaccga tgctgttcga tcatttcaat ctggaagtgc tgccgggcga
     4141 aaagatcagt gtgctcacgg gcacgagcaa aagcaaaacg tccctcgcgc ttctcctggc
     4201 cgggctctat catcccacca gcggcgtcat ccgttataac gacatcgacc tgcgcgacgt
     4261 gtcactcaat tatgtgaatc gctgcagagg cctgatgctc gattcccatc ccaccctctt
     4321 cgatggaacc ctggaggaaa atgtgaccct ccagcgtcca tcgattcaat ttgccgatct
     4381 gagctgggcg ctgcgattcg tggaactgga ggaggagatc gacgcgctgc ccctggggct
     4441 ggaaacggtc gtgcatggga acggggcgaa cttgagccgc agccaggtgc tccgcatcct
     4501 tctcgccagg atgatcgtga cccgtccgga attgctgatt ttcaacggca gcctccacaa
     4561 tatcgacccg accgtgcgac tgactctcct gcgaaggctc tgcgctaaag aggagccctg
     4621 gtccattgtg ttcgtgtcca atgaccccga agtcgggcaa ctggtcgagc gccgcgtcat
     4681 cctcgattga tgggccttgt cgcaaaaccg gcgtcgattt ctttcttgag gcacgatctg
     4741 ctagactgaa tgcttcaacc tcccgggcca tgcacatcgt ttccggcccg acgaaaggga
     4801 cgtccgtgtc gtttgcctcg cgtttcaaat tgtcctttcc cagcacgaat caattgatca
     4861 tcagcctcct gatcgcggga ctgggatgga tcagcggcca ggcgctgagc cgcatcgatc
     4921 aagacttgcg catcatgtat acggaataca cgctgggggc cgcagacctc gcccacatct
     4981 ccgccgacgt catccgttac cgcaacacca tcatccgctc gctggaagcg gaaaatcagg
     5041 cggtcttcga gcgcattacc gaatcacttc cggcgcaacg cgctcgcatt caacatgcgg
     5101 tggatcggta tgcggcagca ggcttgcgcg tctcccgtag tggacgcagc gaagaaaaag
     5161 acattcaggc cgtacgtgaa agcctggacc aatacttcca tgtcgccagt aaaacggtcg
     5221 atctcttgtc gcaggaatgg aaggcgggaa cacccggcga ggcgacagag ctcaggcgca
     5281 aagcggaaat ccatgccgca gacaatggcg gaccgaaagt catgcaagta agtctggcct
     5341 tggaccgcct gctggaaacc gtcgcggaag tcgccaaaga catgcgcgac gaaggcacga
     5401 aaaccatccg ctccaccagt tattggattg tgggagggag ttttttcatc gcgttgctga
     5461 atctgttcct cagtcgaacg accagagcgc cactctccgc atccagacct gaagaatccg
     5521 tccgtcccga ctccttggac ctgcctggag gcggatccaa tacggcactc cgcgccgagt
     5581 agagccgctt gcctctcaca acgacgattc cggcggccgg cctagagcct ggaacagccg
     5641 gcgacgttcc tcctcgctca agtccatcca ctgtcccgca tgaagccctt cgacggtgat
     5701 gtgcataatc cgcacgcgat gtaatgaaac cacccgatag ccgagtgcct ggcacatgcg
     5761 gcggatctgg cgattgcgcc cctcggttag aatgatcctg aatcgacgcg gccctactcg
     5821 cgccatcagg catggacgcg ttcgctctcc taatatgacg acaccctctg ccatgcgcga
     5881 gaggaaggcg tcgtcgaact cgcgatccac ttctacgcgg tactcccgct cgtgcccgtg
     5941 ctcaacccgc aagatctcgt tgacgatatc gccgtcattg gtcaggagaa tgagacccga
     6001 tgaatccttg tcgagccgcc cgatgggaaa gatccgctcg cgatgattga tttccgaaat
     6061 gatgttccgc gtgacgtgca gttcggtcgt cgtcgtcacc ccgaccggct tatgatattt
     6121 gagatagacc gcacggttgc cccgctgcag gatcgttccg tctcgcgcga caacatcctg
     6181 tgacgaaacc tgatcaccga gcttggccac cacaccatta atcgtgaccc ggccttcggc
     6241 caccagccgg tctgcttcac gccgtgaaca caagccctgt tcagtaaaga atttattgat
     6301 gcgcatggcg gccgcgcgaa tgaagaggga ggatgctacg aactgtacgt ctgacgtctc
     6361 agcactgtga gatgagagcc gggagagcga ttcaccatcc gcctaatgaa cccggcggcc
     6421 cgcccgaacc cgccccagca tacgataaat caagcgagca atgctgaact gaggcgcgac
     6481 gaagccttca atgggtgcaa tctcactgat gtccatcccg acgatgcccg gcccgttggc
     6541 cagcgccgtc acgagcgcga gggtgtcgta ccaacctaac cctcccggtt ccggtgttcc
     6601 caaggcggga acaagcgacg catccagccc atcacaatcg aaggtgagat agacaggccc
     6661 ggggcaggaa gccaacacct caggaatcca acgagccgcc cgcccctcgt aggggccgga
     6721 cggatccaaa atcgaggcgg cgaaaaaggt cttgatccgt ggcgtcttat cgatgagctc
     6781 gatttcttcg gggctgatcg accggatgcc gacctgcacc agcggcaaac catcctgcac
     6841 cacgcgggcc atgacactgg cgtgactgta aggattgtcc tggtacgctt gccggaggtc
     6901 gccatgggca tcgatttgca cgacgcacat gccagaatag tgttgagcat gggcacggat
     6961 agcacctaac gcgccggtgt gctctcccgt gagggtcacc aggaacttcc cgcgccccac
     7021 gtgcggttgc acaaaatcct gaatggcctg cacggcttga ccatcgacac gcccgttcag
     7081 gttcagcgtg ttggccgtag cgacaccacc ccactcgcga tacggctcgt accgcaacgt
     7141 ctcatcgtac aactcaacct gttgggaagc ttcgatgatg gcagatggac cccggtctga
     7201 cccgaggatg taactcgacg tgtgttcgta gggagccggc aggacataca cgccggcctg
     7261 atcgggatgg caccagggct cgtcgatccc aagaaaatta tcggcttgcc caagccaccc
     7321 ggacggaagc gccatcgccg ccgctaggcc ttgttccgaa gactcttggg aatgttttcc
     7381 gtggggataa acaccgccaa ggcaatcacg caggtccaac ggtttggccg tcccactgcg
     7441 gattgtgtga tgttgagcgt ccggacgatt tttccgccca tcttgaagac ctgctcgcgc
     7501 tctttccacg ccacatccgg atcgaactcg ataccctggg tggtggcgag catctgagcc
     7561 gccaggtctt cggtgtattc accggactct tcgtccgttt caccataggc atggtgctcg
     7621 gaaagatagc cgtacgtatc acgatcggtt ggaatcgcta agccaatcga cgcggagatg
     7681 agccggttac gttcgttggt ctctgaccgg gccatgacgc aaaaggtgat ctcgccgggg
     7741 ttcaacaatt tttcgccgcg tttgcgggga aggattttgc agttgggagg aagaatggac
     7801 gagacactga cgaggttaca ataggccacg ccggcactac gcaaggcctg ctcgaacgca
     7861 gccagctttt ccttgtgcac tccgacgcct ctcgtcaaaa acatgtgtgt tggtaccatg
     7921 tcggtctccc tcttcatccc gcgtcgaacg cactccaggt gagtgctcga tgaattgtca
     7981 caagcccttg gtcgcgcgcg tgatcatgcg gcgattcgcg cgaaagttgt atcaagtttg
     8041 ttccgcatcc gcaatacggg gggaggggtt tttttgctct gcctgcgctt tcctttacgg
     8101 gcgccggaga ttgacgacca gcaacttcgg ctcggtcata tcctcgatcg catagcgcag
     8161 accttcacgc ccgatcccgg aatctttgat gccgccgtag ggcatgtggt ccgcacgaaa
     8221 ggtgggaatc tcgttcgcca gaactccgcc gacctccaac tgctcgaagg cctgaaaaat
     8281 gcgcccgatg ttggtggtga agattcccgc ttgcagcccg tagtccgact cgttgacggc
     8341 gcgcaacgcc gcctcaaact cacgataggg cgtaatcgtc accagggggc cgaacacttc
     8401 gcggcaggag acgttcatcc cggcagtcac ctgggataag acggtggggc gtacgaccga
     8461 tccttcgcgc gacccaccgg acagcagacg cgcgccctgg gtaatcgctt cgtcaatcca
     8521 gccctcgatg cgttgcgcgg caccggcgtc gatcaacgga ccaaccaccg tgcgttcgtc
     8581 gccaggatca ccggttgcca acgctcgcac acgagccacg agctgttcgg taaacgactc
     8641 ggcaatggat tcatgcacaa agatgcgctg aacggaaata caggtctggc ccgcataggt
     8701 aaagccgccg gtgacgcagc gctgagcggc atactccaga tccgcgtccg gttcgatgat
     8761 caccgcggca tttccgccca gttcgagcac gacctttttc ttgccgcact tggctttcag
     8821 catccatccc acgggtgcgc tccccgtaaa actcaggagc ttgaagcggg gatcgacgac
     8881 taactgttcc gcgagcgtgt tgtcgcacgg caggatattg agcccccccg gcggcacacc
     8941 ggccttaagg agcagatcgc ccagcaacag cgccgtcagc ggagtttggg gagccggctt
     9001 gatcaaaatc gagttgcccg ccgccaacgc cggcgctact ttgtgtacga cgagattgag
     9061 gggaaagttg aatggagtaa tcccgaggat cggtccgatg ggaaaccgtc gcgtcacgcc
     9121 ccaataggac tccatgcccg gcgaccaatc cagcggcacc acctcgccgc cgattcgttt
     9181 ggcttcttca ccggcaatcg agagagtctg gatggcgcga ccgacttcac gacgggcatc
     9241 cgtcagcggt ttgccggcct ccgccgtcat cgtacgcgca aattcttcct gccgcgcctg
     9301 gagtgcctgg gcggcgttgg ccagcagcgt cgaccgcgca tagccggaca agcgacgcat
     9361 ggctgccgca ccatcgaccg aagactgtat cgccgattcc acatccgccg gactggcctg
     9421 gcagacatgg gcaatcgtct cgcctgtgta gggatttcgt actggggcaa ccgtcgtgga
     9481 ttgagaccag cggccgccga ttagaaatgg acgtccattt tccaacggac caactcccgg
     9541 agaggacact cgctactgca cggactccac aggaggagcc gcagaggccg ccgcgctgag
     9601 ggccgcctct tcctgctgag cagctacggc tttggcgatc aactcttcag tcccccaatc
     9661 ctgcaccgct gccaaggtaa acgcttcata cgtcgagacc ccgtgacagg tgggacaggc
     9721 gggcatgggt accttgcggc aaaatgcctc gctcaaacag gacatgcaca cccacagatc
     9781 cggctctcca tcctttgcag gcacagtcca aaaagcttgt ttagacccca tatgcagttt
     9841 cctctttcgt ggctagacga tgatgctcgt cacgatattc acctactgtc gttcacgccc
     9901 atgagcggcg ttacttcgcc ttctctcccg aggccgcgag cagtttctcg atctcttctt
     9961 caaatgcggc aaagggaaag gctccgggaa tcgcgagcgc cggattctgg gccgtcggct
    10021 gttgcgtcgt ccgcatcaag atgaagccgg gcgtgccgtg gaatccccag gcattggcct
    10081 cgtcccgatc ttggaaaatc gccttcatat gcggagcatc gcgcaggcac tcccgaaact
    10141 tcgattggtc gaggccgatg gctttggcgt gctggaaata actatccaca tccaaccggc
    10201 catcagcggc gaagagacga tcatgcatag gccagtaggt gccctgatct cccgcgcaac
    10261 gggcggccag agcggcagtg acgccggggc cctggtcggc acgaggataa tctttgtaga
    10321 gaaaccgcac cttccccgtt tcgacgtagc gcgcgtgaat cttcggccag gtttccttga
    10381 aaaatttcaa acagtacccg caggtgaagt ccgaatactc gatcagggtg atcggcgcat
    10441 ccgccttccc ccgcatccga ttatcgaccg ccggcgagct cgccgccaag gccgaggccg
    10501 tcgtgagcac gagcagcagg aagggcgtgg cgaatctgat catccaacct gtcaggggcg
    10561 tcatgcggag acactcttcg cggcgaaccg ttccaataat cccaccatga gcagcggcca
    10621 caccagcgtg gcgtcactga gcacttcggc aaaccgcccc ccttcggccg gcggcacgaa
    10681 cttgccccat gagatgccct cttgataggt gcatccgctt aagccccccc aatagtccgg
    10741 ttcagggcag atccgcacgc catagtggaa acgcggaggc tcgacattga gaccgagacg
    10801 cgtattgctg atttcaatat agggagccac ttgctgtgcc caattgcgag gcacgccgcc
    10861 gccaatcgtg aaaatcccga gccgcgtgga acctagaact tcttctgcat agtgattcag
    10921 gtcgagatag ggattaaaca ccggacaggt ctgatgcagc gcacgcaata ccgccacatc
    10981 cccgccctct ttgatctggc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 ngcttgcacc atgccgcggt caatcatcgt gctaatgatc ttccccatct tggcaatcgt
    11161 catcgcgccc gagagggtca agacgacttt acagtcaggg tcctcgatca tcgcggcgag
    11221 cgtttcatag gcctcgccga gtcggcggcc gccgaacgcc gtcttccgca tagcttccaa
    11281 caattccgta aaagaatgaa tcttctcggg atctagaggc tctagtgcct ccaagccatc
    11341 cttggctccg tcgtgaaact cccgtcccgc catgatgaac gcctcccgaa aaaatctgct
    11401 ctttcatcga gcagaccatt ttatacacaa ccgactgtcg cggggtcaat tgaagcgccg
    11461 accagaggcc ccgttgcgac tcacctattg tcgcgtgaca cgcagtgagg gagcaagaag
    11521 tcgaaccatc cggatacgtg taggaagaaa gacgaggcag gatgagtcac actgtcggat
    11581 gccacccgtc agctgcgtac gccgctgacc agcgtcaata tcatggctgt cagatacatg
    11641 atcgcgatgc cggtaaacat gcctgccgac acttgtaccc gacgcgtcgc cgtgtacgac
    11701 atcaccgtga gcgagagcac cacatagagc agcgaccctg cggcgagcgc atagaagcag
    11761 atcgagaagt aggacgacac gccctgcccg ctcaggaacg tccccacgca ggtgggcaac
    11821 ccggcaatca ggccgagcaa cgccacatcg cgccaggaga tcggcgtttt tccagcggct
    11881 ccgacgatgc cgaatccctc ggttccgttg tgcaggccga accccgccac cagcaaggcg
    11941 ctcagcgtgt actcaccacc ggcgtaactg gcgccgatcg cgagaccttc cccgagattg
    12001 tgcaagccca tgcccaccgc aatcatgtaa ggcaacgaca ggatccggtt gccggaacgg
    12061 gcgccgaaca cctggctgga ttccagagcc actaagccga cgaaactcac accaaggctg
    12121 cccagaaaca ccagccatga gaggggatca cgcgcgcccg tctgctcggt cgcttcgtgc
    12181 atgaggtcga agaagagata gaccagcacc ccgttcgcga ccccgatcag cccaccctcc
    12241 caggttcgtg gcaacacctt tcccagataa agggcagcga aaatcccgag ataaacggga
    12301 atgaggcccg cgaccgcacc gagaccaatg acatcaagca tggcacgctt ctatcagaat
    12361 cagggctccg gatgaaaggt cgaaggtgag aacctcgctt ctcggtgact ccccgaaacg
    12421 ctggcgctaa tcattcgcga ctcacttatc ctcggtggta cagtgactcc tgccggttgg
    12481 gaaaacgcgg tcgtttgtgc ttcctggggc gatctctatg acttaccaac aactcatgag
    12541 cctggcgaac ggatacgccg aatccaaaac actcctcgtt gccaatgaac ttggcgtatt
    12601 cacggccatc ggcacgggcg gacggagcgc tgaggaactc gccgtatcgt gcggaacggt
    12661 gcgtgagggc atgaagctat tactgcaatc actgacaggg ctaggactgc ttcgtttgaa
    12721 agccgggcgc tactggaata caccgctggg tcggacgttt ctagatggcg ggagccctct
    12781 cgcgatcacc aacctactgt ggctcctcaa tcaccattgg gtggattgga gcggcatgac
    12841 caaggcaatc cgacgtggcc cgcctggatg ggcccccgtc accaagaccg ctaatttccg
    12901 gcgccgtttt gcactcgcga tgcaggagcg tagccacata ctggtccaac cgaccattga
    12961 ctcatttcga ctgcctcgac aggccgaaag atttctggat ctggggggcg gagcaggatc
    13021 ctatgcgatc gcactagccc ggcggtatcc cacacttcag ggtctcgtca tagaccagtc
    13081 cgtcaccgtc gccaaacgcc tgatccgtcg agagcgactg gcgaaccgca ttcaggttca
    13141 agagggcgat cttcggaccc tgccgcttcc cactgacctt gacgtagtcc tgctcgccaa
    13201 cgtgctccac gactttaata agacggagaa ccggcacttg cttcgccgta cccgaaaggc
    13261 gctgcggcct ggcggcagac tgttcattgt ggagtatttt ctcgcgagag aggagacccg
    13321 tcctgctgag gcggccgtct tttcacttct catgtttgca tttactggaa caggccggtg
    13381 ctatagctgg aatgaggtcg aagcgtggtt gaggcaggca ggcttcggtc gttgccgccg
    13441 acataaggtg accggtagca tcggcacctt ggaagcaacc aaactgtagc cagtctgttg
    13501 aattggactc aggccgttcc gccgacacgg ttgtggtagc ggttcaggtg cctccgcacg
    13561 gggatgagta atcgtggcgg aatctgagga cagacctcgc gatcgaacga gaccatgcgc
    13621 tggtagcgct tgacgccaag agccgcctca gccagcgccc aatatagatt ctctacccaa
    13681 cgcaatttca cgacctggct cagataaccg tataacacct gtctggcgcg gatggctccg
    13741 cgaaacgcgt ccttcatctc gtgaagaagt cggtcctccg catcggtccg ttgatacacg
    13801 ccctcgacca tatggttgag cgagataaag tcctgaagat cgaagcgagc atccacatcc
    13861 ttcgtctgtt cccgatagag ctcccaatcc gtggtgaact gtaggcgctc gaggagaaga
    13921 agaaaatcct gcaacgcgat ctcctcgacg gcctcgcaat ctcgcgagac ccgcactttg
    13981 acgaacgttc ggcgttcgta actatgcgtg ggcagataat gtgtggcaga gacttcccgc
    14041 aagcgtacgg aggcatcatg cttggcgtgg tgatactgcc agagaggggg aaagttcacg
    14101 ccggcctgct gtaattcggc atgataccgt ggaagcagat gctggaacag cggcccaccc
    14161 tgttcatgaa acggcgtgga cggcagccca cccacagcta aagtgagtcg cgcaaagggc
    14221 atccgtgtgt aaccgtccgg ttcaagcacg aaattgaatc cgccccaggg ccctacgtcg
    14281 agcaacgtgt catacccctg acttgcgagc cattcttctg tctccaaggc aagccgtcgc
    14341 cacagccgga tcagcgaccc gatgtgcacg tgccgattgc gaaagagagg tactggaacg
    14401 accggattgc cataggcact gctttccaac cgtgtttgga gcttcagtat ttcccattcg
    14461 agcgcatgcg ccaacgtgcc cttcccgtga cggatgccgg agtattcccg ctcgcattcg
    14521 acacgaatgc catcccagag atcggttccc cgaccggagg ttcccgcgac acacacgatc
    14581 tgatcgatga cccgaagtaa tcctggcggg agccccgtcg gcatgatgcg atgtacaccg
    14641 atccgaaact ggcgaagagc ccgcaaagtt cgccgttcat atgccagctc tgggcccaca
    14701 ttctctattg ccgtctgagc gagacttccg tcgaacagga tttagctcct ttgctgctct
    14761 tggcaggacc cacaaacggt atcaaaccac cacaggtctt caggcctcac acgccaggct
    14821 tccatggcca ccgcttcctc ctgccacatg tcgggaaaag actcctccgt tcctccagcc
    14881 atcacccgat ggcattgctt gcagtggaac cagacagggc gcccgaataa tgccaccaga
    14941 aaatcgttcc ggttcatggc caccattcct gccaatagac atgtctggcc atcttcggat
    15001 ggcgtgccac gcccagacac gagttgtacg gcaccctgct ggaaggcgga actcgtaaag
    15061 cccgatagtt tgtatgagaa gattacgagg tacgaacagt tacagatgct gcgtctgatt
    15121 gactgaaggc gtgaggctcg tccacggcct ggagagaaga gcgttcaata ctggacatcg
    15181 ggtgacaaga gccgaagcgc ggagcagctg ttcgtcattg acggaggaac tgcccggcgc
    15241 cacaggcacc atgcccatga cgccattctg cggttcatca tgagttgtcg gcttggacat
    15301 acccccccta cgtcagacgg tagtctgcac gtcataaaga gaataggccg gtctcactgc
    15361 atgagtaggc cggaccgctc tctcgacgat gctttcatag acggccgtat aggctgtcgc
    15421 cgtttcgacc agtgagtcca ttgtttccgc agggacttcg ctacgctgac gggggccggg
    15481 aacgggaata agagctagcc aattgagctc cggacactgt cgaatttgct cggcggaggt
    15541 tgccaccaag ggcaattcac gaaacgctct gaacaattgc aaaatttctg gtgcatccag
    15601 cgagccatag accgtcgcca cgctgggaag aggactgcgg cgcatcatcg gaaaggcgac
    15661 aaagcccgca tggacgtgga gaaggtcaaa ggaagactcc atggaaaacg ccttttcgat
    15721 agccagaaag gtcaatcctt cggagagatg ccgcttgggc gacggatagt gccgaatcgc
    15781 ttgaggcgac accggtatta atctacctga tactcgactg tcgccactgg cgaagaccgt
    15841 cacgtcgtgg ccaaaccgca tgaggctcct cgtcacgaac tccacgcccc tgacctctgg
    15901 tctctcactc tcgttcgaca cttcctcgaa acactgactg atctgagcaa tgcgcataac
    15961 tgttcccttt ctcggtcgcc aaaccggttg aacccaaatc gatccgtgcg atgcgtgcac
    16021 agcgcaagct gcccgaatag gcattcccgg aaaccgacgc ttctggcgag agacgggatg
    16081 gtacggccca aagatgaagg ccgactgaag acagcatgaa attttgatga gaaaaccagg
    16141 ggcacgtgat gcgaggtcag cttcggcgca caatgcggag agacacacgg tagcagttac
    16201 atcaggaagg agccagcaaa ccgtgggtca acgcatattt gatcaattcg gctgtggtcc
    16261 gcagattcag ctgctccata acttgggctt tgtgaaactc gaccgtgcgc gtggaaatct
    16321 tgaggcggag cgcgatgtct ttcaccgttc gcccttccgc caataactgg agcacctcac
    16381 gctggcgaaa ggtgagcgtt tttttctgtg gcgtctgtcc cggacctgcg gtgagcaaca
    16441 catcgaggag atctttcgca atcaacggcg tgatgtagct atgtccggcc cacacagccc
    16501 gaatggcctg ctccaattca tcgaccgccg accgcttcag gagatacccc gacgcccccg
    16561 cctcaaaggc ggatcgaaca tagtcggcgt ccgaatgcat cgtgacaaaa atcaccttca
    16621 tcgacggatg cgttttcttg agctgcgtgg ccgcgtcgat gccgtttaag aggggcatgg
    16681 acacatcgag aatgacaatg tcgggctcga gccgagccgc cgcctcaagc agtgcgcggc
    16741 cgtcctcgac catcccgatc agttcacact ggtcggcgag aatgcggcga aatccttcga
    16801 gtaccagcga atgatcatcg gcgagcaaga cgcgcggttt agacatgctg actccctgac
    16861 agcggcacgc caacttcaat atgcgtcccg ccccccggcg acgacgagac attcaaggtt
    16921 cctcgcacga gccgcacgcg ctccttcatg ctcaacaatc ccaggcgccc ccgtccctgc
    16981 gacgcatgca cggcatcaaa cccgactccg ttgtcccgga ccgaaagagt aatcatcccc
    17041 tcgtcacaaa tcaattccac ttcgacctcg gtcgccttag catgtcgggc cacattgttc
    17101 aggctctcct gagcaatccg atacacgcac gtggccagat cggttggcac gggactcaaa
    17161 caatcctggt agacatacac cgcttcgatt cccgtgctgg cagaaaaatc atcgaccaat
    17221 cgccgaaccg ctttcataag gcccagatcg tctaaaatgg agggatgaaa ccggtacgcc
    17281 atctgccgaa catcatccga cacggcagtc aaccgaccgc tgatggattt taccatccca
    17341 ccgatattcg aaagatcccc cgcttcaccc ttttcgatcc gcctgagatc catcgctaac
    17401 atggccaggc gctggttgac atcatcatgc aagtcacgcg caatgcggcg ccgttcgacc
    17461 tcttgcgccg tcagcaattg ccccgccaac gcccgcagtt cttcacggct ttgtcgcagc
    17521 tcctcctgac tcaccttcaa ggaagattcg ctggcacgaa gagaccgctc tgtttccatt
    17581 cgctcctcga tttcccccac gagcgcttga ttgaccagtg ccagctcccg cgtacgcgct
    17641 tccactcgta tttccagttc attgtgcgcg ttgcggagcg cctcttcact ctttcgccgt
    17701 tgaaaggcat agacgaccgg aaaccagatc gccgtgaggc tgaacatgcg attactcagt
    17761 cccatccaga gcggcacgtt gggaagcgtg acaccgagaa acacatccga cagggcgaag
    17821 accgaacagg tgccggccac cagtagcgga atcttgcgat cgggaattgc cagcgacatg
    17881 accacaaggc cgccatagag caccccgttt ccgattccca acgggagata gagatcaagg
    17941 gtatagagac caatccccag gatgataatc gtgagcagaa gcagtcggtg ggaaggctga
    18001 gtcatggatg agcgattatc ctcccctcag ccggaaggct tcaagcaaag acacaatcag
    18061 atggagggaa ttcgctctcg ctcaggggca gctcaacggc gatagatgag taccgtaggc
    18121 agaacgaccg gcgagggcgg cacagaaagt caccgccccc gaccgttcac taccgtttcc
    18181 gaaaagcgga aggagcgaac cgattaacgt gtgccggcga gctgggccag ggtcggatgt
    18241 ccggtcatcg cctcctgcaa cgccttcacg accgccactg aagacacctc tccagcctcg
    18301 gtcagcgcga gatactgctg ctgcgtcaat tgaaaaaagg ccggctgctg ttcgaccggc
    18361 acgttcatca aggttgcgag tgcggtcagg tgttctccac cacccctcgc catgtcggtc
    18421 gacagcgtgt cgaaggtgct ggcgacaaag acattcgtct cctgcgatgc catcactttt
    18481 ccgtcattgg tgcaacccga ggtgccaaaa ctgatgccga acgtttggct gccgaacgtc
    18541 ccgttggtcg tcgccatcat gacttggggc gcaatatttt tcggcgtctt gtaatcagac
    18601 caagccaact tccccaatcc gcatccaggc ccgttgtctg gattcaccgc gaaggctgtg
    18661 ccggattgaa gaccaatcaa cagacctgca agcacgagga ttccttgttt cttcatgagc
    18721 tccccccttc tgttcttcga tgtctgtgct acgggcaaaa acggttagcc gctttctctg
    18781 cgcgatttct accttagctg tctttttcac cctgtcaatt gaagcgccgg ttttttcgtg
    18841 caggaagaag aaaccggcgg gggtgctgat cgctcaagta tctgcgtgag actttcactg
    18901 acaagaaagg cgaacaacgc gtttcgtatc gtgcggctgg tgcggaatcg ctcagtgcgg
    18961 accagactcc ttattccgtt gaattggatc tgagctgcta tctgtgaggc gctcgctaga
    19021 gcgacgcttc atggatggcg ggatttcacc ggttctgtcg gtgcatcgtg cgtgcgacag
    19081 gaatgctcaa ggacaggcgt ttgtcttgca tcgaataccg ggcagaaata tcgttccgac
    19141 tatcgcttca tgccaagcac tgcaccgatc ccgccacatg cccggcctcc tcgcatcgcc
    19201 agcatcgaaa gctaccgcgt tgtggcgatc ctgcttatcg tatccctcca tacaaacctg
    19261 attgcacgtc tgcatctcat cggcggcggg gttggcttcc tcgtggacat gccgctctat
    19321 ctattgttct ggatctccgt cccctatttc tttttgacgg cgggatactt ttacgggcgc
    19381 accgtggatg ccggagcagc ccccttctcc ctactacggc gctcgtgtcg ctcgctgacg
    19441 ctcctctttg tcatatgggt cgctgtgtac tcgatcatcg gaccgaactg gatcagcaac
    19501 gtctacacac aagggctctg gtcgaccatt tcgacggagg cctcccatac catgggcagg
    19561 ctcatgcagg agcatgtcac actacttctc gtgccgagac ctccgatctt tcacctctgg
    19621 ttcttgccgg cgctgatcgc tggggtgagt acggtcgcaa tggtcgtgac cagccgtctc
    19681 aaaacatggg tgggggctct gcttgtctcg acctatatcc tgctcgtcgt gagtgcagtc
    19741 gtt
//
LOCUS       DAOQEW010000054        19732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_693_length_19732_cov_7.963307, whole genome shotgun sequence.
ACCESSION   DAOQEW010000054 DAOQEW010000000
VERSION     DAOQEW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19732
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_19732_cov_7.963307"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..452)
                     /locus_tag="PLS35_07960"
     CDS             complement(201..452)
                     /locus_tag="PLS35_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNI18632.1"
                     /translation="MSTKGREAAKIAAIIGGGAVVGAALGLLLAPKSGAETRRDVARY
                     AKKAQLQATRFSRSVKSGVSTMVERSKALVKKDDHKEAA"
     gene            779..1612
                     /gene="lgt"
                     /locus_tag="PLS35_07965"
     CDS             779..1612
                     /gene="lgt"
                     /locus_tag="PLS35_07965"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNI18633.1"
                     /translation="MLPWLHAIPYPDIDPVFLRLGPLQFRWYGLMYLIGLTLAYFIIE
                     ARARAQKLPLNKDQVYDMIVYAAVGVFAGGRLGYVLFYNLSYYLENPLKIFAVWEGGM
                     SFHGGLIGTIVALILFAKRQGMTVLTIADLAAGVTPVGLGLGRIGNFINGELYGRPTD
                     VDWCMVFPAGGMVCRHPSQLYEAFLEGLLLFTVLWLITRRLPPSGTVFGSFLVGYGLC
                     RIVVEFFREPDAQIGFIVGTISMGQMLSIPMIVAGAVILAIASQRKRPLRSDSSSTAT
                     L"
     gene            complement(1638..2042)
                     /locus_tag="PLS35_07970"
     CDS             complement(1638..2042)
                     /locus_tag="PLS35_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18634.1"
                     /translation="MKHLGWMIMVIVTIVGCAGPRKAEVRPLHAPTGTPEAVSRQLEQ
                     GNQLLSKEDWAGARDAYLATIQADQSLAEAHYNLALALEKLGEKAEARKHYVAAANLA
                     PGNKVIWDAPPLRKYDSELTLGKKSWMDANPK"
     gene            complement(2058..5450)
                     /locus_tag="PLS35_07975"
     CDS             complement(2058..5450)
                     /locus_tag="PLS35_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNI18635.1"
                     /translation="MTEQSTIPDQAAREAAATTFDRNVVVIAGAGTGKTTLLVNRLLY
                     LLMRRADPLDLSRIVALTFTNKAATEMKLRLRERLRSLVSLEGRDNPATGSGTVSIAD
                     LRLRFGWTTDEIVARAEAALRDIEKAQIGTLHSFAAHLLRLYPIEAGVSPTFQTDEDG
                     SRFEEHFTAEWELWLDRELGLSGSDHGRWRLLLAAFSLEDLRQLAYSLHSDLIDLDGL
                     RAQITENGLNQVLRDWFAERKAQAARLLARYDGPKRRKIESMLATIVDLFDLLLDDGL
                     DGIHALPPDSHELLAKELGKAPNGWVEEDFEEAESLQRIANRLLKVDQGLLGQLLEVL
                     APFIRAVRKSFLDTGWLTFDGLVGKARSLLRDYPMIREQLKRDYRALLVDEFQDTDPV
                     QYEIVLYLAERLGEQSTSWRTIELESGKLFIVGDPKQSIYAFRRADIEAFDHVVERLE
                     QGGALRCELATNFRSHVQVLDLVNGVFNRLLVAQPSVQPPNVPLTVQPNRSSQFRDTG
                     VELRLVQSEEEGDLDSAAATRVEAEHLASMISRWLTPTEPGGEAAVAGTRLRPGHIAL
                     LFRKLTQAEEYLEALRRHDIQYIIDGEKHFYRRQEVIDLVNILRCIENPHDRIALVGV
                     LRSPIGGLPDSALVSLQEAQAWDYREPGPLSKWTSPHVEPLRRLYAVLARLHEAAPRC
                     PLPQVMDLVFQQLPLLELAAASIHGEQAVANLLKIRQMAADLADRPALTLNGFVELML
                     ERLREQPDEAESALSEDTLDAVRVLTIHKAKGLEFPLVILAGLHHGDGAGRGPARPQI
                     WHDWSTGTQGLDFGGLCTAGAVLVAEKARIREQAERRRLFYVGMTRARESLVLSGALP
                     KRRVRGALLDLLAQAAGAEFNGIGEGGLSVGGVGLRHTILKGEDRFPSRERPQPTMLE
                     ENTVDQALCDRWVQRDRVWEEHRSQSLIISPSDFIRKPAPLPFSNIQPRQGRGSTSGT
                     AIGTIVHRVLQYWDFTADAERQVAAISSASLAIEEQESTTRQAIVEDVQALLHIFIQS
                     PAYARLRQATVIGREMPFLIPWQDGRQILEGAIDILYRIEGKLWIADYKTDMIPLDQV
                     TGRADLYREQARLYREAVTQSLGEPVAGFEFIFLRLGVTVPV"
     gene            complement(5443..8673)
                     /locus_tag="PLS35_07980"
     CDS             complement(5443..8673)
                     /locus_tag="PLS35_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNI18636.1"
                     /translation="MLRLVTGPFHPTLETRLVQDLRALKSKDRLASLAIIVPSDQLRR
                     SLKRLLILQEKLSLLNVHILSFHQLALHVDRERRTAEGAAPGRQMDLVSDVFFEHLLR
                     HIGQRRMPHTESLRLSQLPHGAWPALWASLRDLKDATVDSAVAVRAVEEGQFAPEEGE
                     KLKGLFTLYAAVREGSAALGVGSPDDLASLVTPFVPTSPFLRGLHELWYYGSYDLTQT
                     QLTLLESLAATLPVTVYFPVDAQPAYGFARQFLERHLYPMAGTSDASMLDSPEPSGSD
                     GTRANVSVEVRNAAGIDDELTLVCKQILTLVETHGYRFEEIGVVGRTLVPYQSTLVRL
                     FDQHRIPFTSTAAVPLLREPAMKTLLHLARLKGNGLHRSAMLDVITSPWNRRLTAPQG
                     GEPPRPDLWRLAVHALGITKGEEEWRRLAQLGRLEAWAGTGDESSEDPLDALSIDGPQ
                     LRLLWDCVSPLIDGVTGLPESGGYGGLTDAFCALAEDHLSIPLDMSDSIDAATQSQGI
                     TDVTEALVGALAQLRELDRLDVRITWQEWTDTFEGILERTTYALTSPSQQGVQVLDAM
                     AARGLGFRALFVVGMNEKMFPRYIHEDGFLRDSHRLVLSETLGYKIDQKLQGYGEEAL
                     LFELLRASARERLYLSYQRADAAGRPLAPSGYLDGMGVVPHAADAQSLFAVPRRWKDR
                     TALPLFIPPLLTREEHTVSSVLHGRDAASLLNAGGREGELFAHGLAAQALIESGVPAL
                     SAYDGLLTDATSHWNRIRERGISPTALETYARCPFQYFSAQVLDLDAVRQTVSMELSP
                     PAMGQLCHDALRLCYLALIRQGWGTAASSAAVVTAEVTRAVTQAFTVYAMTHGTGYAL
                     TWQLAQDTVRHLVEAMVRFDCEAAAASGFIPVECEIDAKGVLPDGTAGEAIPLRGRWD
                     RLDRHPVSGALRVIDYKYRANDRIDAKDRQLLQAAVRAQRLQPALYTLMAVDGPGEQP
                     HGPQPEQVEFLYLLPQGMPMVERASFPASCWQGPSGPMLSRTVQLLVDGIRSGRHFIV
                     PDTAHCDHCEFATACRKAHQPSLWRTRRSSLAGALRALRFEKVARD"
     gene            complement(8770..8934)
                     /locus_tag="PLS35_07985"
     CDS             complement(8770..8934)
                     /locus_tag="PLS35_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18637.1"
                     /translation="MTVPDDDDFELPILPMIDKGPPPECGTCGDPMKFIDGDWACVDC
                     NGELLGPETG"
     gene            complement(8947..9213)
                     /locus_tag="PLS35_07990"
     CDS             complement(8947..9213)
                     /locus_tag="PLS35_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNI18638.1"
                     /translation="MPNITLLHSPTCGACPSAKRLWKELRVKYSFSYREVDITTPDGQ
                     ELANRHSVRAVPATIIDGRLTFVGVPPRQSAEKALQLKMKPQGA"
     gene            9370..11022
                     /gene="pgm"
                     /locus_tag="PLS35_07995"
     CDS             9370..11022
                     /gene="pgm"
                     /locus_tag="PLS35_07995"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HNI18639.1"
                     /translation="MAQHPLAGQPAQPEQFVDLARLEQAYYQETPDPADVQQRVSFGT
                     SGHRGSSLKRTFNEAHILAITQAICEYRIKAGTTGPLFVGKDTHALSAPAQRSALEVL
                     AANGVEVRLDKDDGYTPTPVMSHAIIQTNRDRTSGFADGIIVTPSHNPPEDGGFKYNP
                     PHGGPADTDVTKAIENRANELLAANLQGVKRMPYEQAIRAVSTTRYDFAGTYIADLAQ
                     VVDLDRIKAANLRLGVDPLGGAAVAYWRPLAERYGLNLEIVNASVDPTFRFMTLDWDG
                     KIRMDCSSPYAMASLITLKNRFDLAFGNDTDTDRHGIITPGAGLMNPNHYLAAAISYL
                     FTHRPGWKSSAAVGKTVVSSSIIDRLVRSLKRRLVEVPVGFKWFVPGLSDGSLGFGGE
                     ESAGAAFLRRDGTTWVTDKDGIIMNLLAAEMLAVTGKDPAQLYGELTAQLGEPVYERI
                     DAPATRAQKAALQKFSPQQVRSKELAGEPIIAMLTEAPGNKASIGGLKVTTANGWFAA
                     RPSGTEDVYKLYAESFKGQAHLLQIQEDAQALIKQVFSSAGA"
     gene            11202..11903
                     /locus_tag="PLS35_08000"
     CDS             11202..11903
                     /locus_tag="PLS35_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNI18640.1"
                     /translation="MPTLSRAWCSLALASALTACAAPSSQGLRQAVTPIPDCCRASHS
                     DTRQQAIVRTAVNLVGATTIESQGRRISYDCAGVTRAIYLAHGIDLYSDGSADGRTNG
                     VGLIYNHVRKNGRLHRGPVVQAGDLVFFDNTWDFNGDGLVNDPLTHVGIVEQVEQDGT
                     IVFISRVAGAIERYRMNVAHPHIHRTADGRLLNDYMRRKHWRDGEHTAYLTGELFAAF
                     GTRVIDSPSIPPAPR"
     gene            11909..12109
                     /locus_tag="PLS35_08005"
     CDS             11909..12109
                     /locus_tag="PLS35_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNI18641.1"
                     /translation="MSAIQTSPHRCPECHQATAWKDNPWRPFCSERCQLIDLGAWAGE
                     QYRVPGPSLSVGGTESFSPDSE"
     gene            complement(12125..12490)
                     /locus_tag="PLS35_08010"
     CDS             complement(12125..12490)
                     /locus_tag="PLS35_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18642.1"
                     /translation="MLRELLRTGVAVALLVSVTLLPALTSRIQAEEGGASNPSSVTQS
                     PAPRPGALQQTPAPVEPSLPLLMAEGSNSCEIVSCGMGIKQTCQITCPADKTPKCSCD
                     CERSFGPMCMDYKANCRCE"
     gene            complement(12558..13769)
                     /locus_tag="PLS35_08015"
     CDS             complement(12558..13769)
                     /locus_tag="PLS35_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNI18643.1"
                     /translation="MTTRSVHRRAFLQAAMALPLLWLRPGWSSPLRAAGTSDFSERSH
                     KRERILLLVELQGGNDGLNTVIPYDDAAYYRARPQLAIPRDQVRQLTPKLGLHPALSP
                     LMPLWERKELAWLQGVGYPKPNRSHFRSIEIWDTASDSEEVRDKGWLSGLFAQYPLPA
                     GFTAEGVLLGRGDAGPLSGGTTRTMALHDPAQLFRQAGALKPLSASTTNRALAHILEV
                     RREISQAAIDVQQRVQEAPPFAMDFPTHKFGKQLEVAARLIAAKLPVAVIKVTHGSFD
                     THAGQLSTHHRLLEELTQGLMAFRAAMEQRGLWSQVLVMTYSEFGRRVGENASHGTDH
                     GTAAPHLFMGGRVRGGLYGAPCSLTDLEGGDLKYTLDYRSLYATVIEQWWGLPAVSFG
                     GGRYPVVDCLT"
     gene            complement(13777..15099)
                     /locus_tag="PLS35_08020"
     CDS             complement(13777..15099)
                     /locus_tag="PLS35_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNI18644.1"
                     /translation="MGLTFDEARHLLSRTGFGGTPEEIRAFMAFDRPDAVKQLLTIPT
                     HKASTPPPAWIHQLPPLPRLRKFWTDRERKAFHEQLKVQGHELKAWWYRELITTRHPL
                     LERMTLFWHNHFTSSLHKVRWPAFLYQQNVLLRLHALGSFRTLLQSIAKNPAMLLYLD
                     THTNRREHPNENFARELFELFTLGEGHYTEQDIKQAARAFTGWHLDHHTGAFRMDLRH
                     HDEGQKEVFGRKGHFDGDDILSLTLEQPHVARYLVRKLWREFISEQPDAVAVERLADS
                     FRRNKYETVDLLKELFLTEAFWAPENRGVLIKSPVELIVGTMRLFDLPFDDVTLQVVY
                     GRRLGQDLFDPPNVKGWPGGLRWITTSTLLSRTQLLQRAIRGHETGHLHGMTDMKQAQ
                     GAMWLAMETTEVAQATLLPFAPVQPLTAGDDRGQAVHHLVLDPVYQLK"
     gene            15240..15671
                     /locus_tag="PLS35_08025"
     CDS             15240..15671
                     /locus_tag="PLS35_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18645.1"
                     /translation="MRFALASLLIVLTIGFGTGCSTPSTSKLTGAVRYFNIRSEIAPR
                     HLIAQIGDEIRWQNLNPHPVTLRMLEDSDPALLACDKGFKQFGVIQDTVTIAPLQSVS
                     LCFLKPGTIRFNVWLDADNPQGAMTPTGTIRVEPSPVRRNS"
     gene            complement(15799..16224)
                     /locus_tag="PLS35_08030"
     CDS             complement(15799..16224)
                     /locus_tag="PLS35_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18646.1"
                     /translation="MMQQGGRTWSIQRSVLRISVCLLLLAPALVTAQSLPPSSNQLNQ
                     QNQPGSNLQAPGTIPIPNPLAQQSAISSTASGASAPQSNTIPSSQGKSFGTIGQGLPG
                     MTSGPTLTAPMGSQDPSSRYMRPTVIPPLFCDPAINIPC"
     gene            complement(16244..17350)
                     /locus_tag="PLS35_08035"
     CDS             complement(16244..17350)
                     /locus_tag="PLS35_08035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNI18647.1"
                     /translation="MAQAKPKKNGAVAKKLVGMADVVISAAQRSKDPTFEIPIRALSN
                     VSFNPRKGLIEMGEKKQARTFFNVGMAKKFMQTMLVADALAELQQADLTTSLREIYYR
                     TKHTIQDSHENTFDTQDESDPIIEDLEVSLIALREELHVRAENSGSIVGPVVFGDDGD
                     RVDCAKLGKGGYSVPSIVEPEYLEIRRCTADFLLLVEKGTQWNRLSEDKFWRRYNCVL
                     LTGNGQPPRGVRRLARRLHEEHKLPVYVLVDNDPWGYYIYSVVKQGSINLAFESQRMA
                     IPKAKFIGLSSADPERYELPRNVGIKLNEKDVTRAKELLNYQWFQKPAWQQEIKRMLT
                     SGLKYELDALANKDFQYLTKKYLPRKLKEKDWLD"
     gene            complement(17359..19353)
                     /locus_tag="PLS35_08040"
     CDS             complement(17359..19353)
                     /locus_tag="PLS35_08040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HNI18648.1"
                     /translation="MTAVEMGARQREISVSEFFTKNRHLLGFDNPRKALLTCVKEAVD
                     NSLDACEEAGILPDVTVKLEVVANGPAPVAPSQATRFRITVTDNGPGIVRQQIPRVFA
                     KLLYGSKFHRMRMSRGQQGIGISAAGMYGQLTTGKPVKVISRTGPRAAAHYFEVQIDT
                     KKNEPLVHENKQIEWSQPQGTEVVLEVEGRYQKGRASVDEWLEQTSIANPHVRLTYHT
                     PEGEQRSYPRTYHELPPSPKEIKPHPYGIEFGALLKMLQDTKSHTVASFLASDFCRVS
                     PALAEEICKAAKLSPTTKPRDVKPQAAETLYKTIQTTKIMAPPTNCISPIGEKAILSG
                     LYKQIKGEFYTAVSRPPAVYRGYPFVIEAGLAFGKGPEEPSKPQHAAVPLAEGEDQGN
                     DTEQARVIRYANRVPLLYQQSACATFKAVLSTTWRNYGVSQSRGALPAGPMVIFVHMA
                     SVWVPFTSESKEAIADYDEIQKEITLALREAGRRLGIFLRRRERAASEFRRRNIFELY
                     IEEVVAACNRLKGGNLPTEKLKEQLHKIASSRTGGLKTDEALGKTGAGPEGLPHSIIV
                     TAEGIEGEREVAENVPEPADGPVSAASPESAAPAKVPKKGRRAVATAPTSKKSRPASN
                     AKRKAGQQELFAEAADGKHAPARKTPSKPSKTATGTRKRK"
BASE COUNT         4021 a         6132 c         5574 g         4005 t
ORIGIN      
        1 ttggtatgac acaggatttt aagtcctggg tgtctgccga ttccaccact cccgcgtcaa
       61 aaaccgtcgc ggcgaaatct aacacccggg taccggaggg tcaagctgct ggagagaatc
      121 tttactatgc aacgacctgt cccgtatgaa acaggccgtt gcacaccagt ggtttgatcg
      181 cctgttttct cactcgccgg ttaggcggct tctttgtgat cgtctttctt caccagcgcc
      241 ttgctgcgtt ccaccatcgt agacacaccg cttttcacgg agcggctgaa ccgggtcgcc
      301 tgaagctggg ctttcttggc atagcgggcg acatcacgcc gcgtttccgc accggacttc
      361 ggcgccaaca ataaacccaa cgccgcgccc acaaccgccc cgccaccgat gattgctgca
      421 atcttggctg cttcacgccc cttggtagac atcgtgactt cctccctctc gtacaacaag
      481 gttgatggtt cactgacttg gcgagtagct atctcgctat acagcatcaa gcgtgccaga
      541 cgatgcgtat gtgcggttcg gcctgatttg tgtgattcgc gagctgcacg ttagagacgc
      601 ctcataaagg gtgctgtttt gtgaacaaca gcaggagaca accgtttcag aaaagcgaca
      661 ggtgtgcgta ggaaactttc atgagtgccg ctcacgcctt gcctggcgcg ggctcgccgc
      721 ttgccttgca atgtcgagca cgctcacata agatcgcgaa actatgtgga gtccttctat
      781 gctgccctgg ttacacgcca ttccctatcc ggatatcgac cccgtatttc ttcgattggg
      841 cccattgcag ttccgatggt acggcctgat gtatctcatc ggactcacgc tcgcctactt
      901 catcatcgaa gcgcgagcga gggctcagaa actgccgctg aacaaggatc aggtctacga
      961 catgatcgtc tatgcggcgg tgggcgtgtt tgccggcgga cggctgggct atgtgctgtt
     1021 ctacaacctg tcctattatc tggagaatcc actgaagatt ttcgcggtct gggaaggcgg
     1081 catgtccttc cacggtgggc tgatcggtac catcgtggcg ctgatcctgt ttgcaaaacg
     1141 gcaaggcatg acggtgctca cgattgccga cctggccgcc ggtgtgaccc cggtcggcct
     1201 cggactgggg cgaattggca actttatcaa cggtgagctg tacggccgcc ccactgatgt
     1261 ggactggtgt atggtatttc cagccggcgg gatggtctgc cgccatccct cacagcttta
     1321 tgaagcgttc ctggagggcc tgttgctgtt tacggtgctg tggttgatca cccgccgtct
     1381 gccgccatca ggaaccgtct tcggatcttt cctggtcggc tacgggcttt gtcggattgt
     1441 ggtggagttt tttcgggagc cggatgcgca gatcggcttc attgtcggga cgatttccat
     1501 ggggcagatg ctcagcattc ccatgatcgt tgcgggggcc gtaattcttg ccatcgcctc
     1561 tcaacgcaag cggccgcttc gctcagactc cagctcaacc gcgacgctct gagcagcatc
     1621 atcgtcgcac aaggtgttta tttcggattg gcgtccatcc aggacttctt tcccagggtc
     1681 agctcgctgt cgtattttct gagcggtgga gcgtcccaaa tgactttatt gccgggagcc
     1741 agattggctg cagcgacata atgtttcctg gcttcggctt tctcgcccag tttctccaag
     1801 gccagcgcca aattgtagtg cgcttctgcc agtgattgat cagcctggat cgtcgcgaga
     1861 taggcgtctc gcgcgccagc ccaatcctct ttgctgagca actggttgcc ctgctccaat
     1921 tgtcggctca ccgcttcggg agttccggtc ggcgcgtgga gcggccgcac ctccgctttc
     1981 cgcggacccg cacagccgac gatcgtcacg atcaccatga tcatccatcc aagatgtttc
     2041 atgcctactc cttgtgttca gaccggaacc gtcaccccca gtcggagaaa aatgaattcg
     2101 aatccagcga ctggttctcc cagcgattgg gtgaccgcct cccggtacaa tcgcgcttgc
     2161 tcacgataga ggtccgccct gccggtgacc tgatcgagcg ggatcatatc cgtcttgtag
     2221 tcggcaatcc agagtttgcc ttcaatgcgg taaagaatat caatggcacc ctctaaaatt
     2281 tgccgcccgt cctgccaggg gatgagaaac ggcatctccc gtccgatcac agtcgcctgg
     2341 cggaggcggg catatgcggg cgactggata aagatgtgaa gcagggcctg cacgtcctct
     2401 acgatcgcct ggcgggttgt cgactcctgt tcctcgatgg ccagtgatgc gctgctgatt
     2461 gccgccacct gccgttcggc atccgcggtg aaatcccaat attgcagaac ccggtgcacg
     2521 atcgttccga tggctgttcc agaagtcgaa ccccggcctt gccgaggctg aatattcgag
     2581 aacggcagcg gtgccggctt tcgaatgaaa tccgaaggcg agatgatcag cgactgtgac
     2641 cgatgctcct cccatactcg atcacgttgc acccagcgat cgcagagagc ctggtcgacg
     2701 gtgttctctt ctaacatcgt tggctgcggc cgttcccggg aaggaaaacg gtcttcgccc
     2761 ttgagaatcg tgtgtcgcaa accgacgcct cccactgaga gcccaccctc cccgatcccg
     2821 ttgaactcag ctcccgcggc ctgcgccaaa agatccagca gggcaccgcg tacccggcgt
     2881 ttcggcaagg ctcccgataa caccagagac tcccgtgcac gggtcatccc gacgtaaaac
     2941 aaccggcggc gttccgcctg ttcgcgtatc cgcgccttct ccgcaaccag caccgcgccc
     3001 gccgtgcaga ggccaccgaa atcgagtccc tgcgtgccgg tggaccagtc gtgccaaatc
     3061 tgaggtcgcg ctggccctcg accagcccca tccccatggt gtagcccggc caaaatcacc
     3121 aacggaaact ccagcccttt cgctttatgg atcgtcaaca cccgcacggc atccagcgta
     3181 tcctcagaca atgcactctc cgcctcgtcc ggttgctcac ggagtcgctc caacatcaac
     3241 tccacaaacc cgttcaaggt gagggccggc ctatcagcca ggtccgcggc catctgtcgt
     3301 atcttcagca gattggccac cgcctgttct ccatgaatcg aggccgcggc cagctcgagc
     3361 aaaggaagct gctgaaacac cagatccatc acctgcggca atggacaacg gggagctgct
     3421 tcatgcaacc tcgccagtac cgcatacaac cgtcggagcg gctcaacatg gggactcgtc
     3481 cacttcgaaa gcgggcctgg ttcgcgataa tcccatgcct gagcctcctg gagactcacc
     3541 aaggcggaat caggcaagcc cccgatcggc gaccgtaaga caccgaccag cgcaatacgg
     3601 tcgtggggat tctcgatgca gcggaggatg ttcaccaggt caataacttc ctgccggcga
     3661 tagaaatgct tctccccgtc gatgatgtac tgaatgtcat gccgtcgaag ggcttccaag
     3721 tactcctcgg cctgggtcaa cttccgaaac aacagcgcga tgtggcccgg gcggagcctg
     3781 gtgccagcca ccgccgcctc gcctcccggc tcggtcggag tcagccaccg ggagatcatc
     3841 gaagcgagat gctcggcttc caccctggtc gcagccgccg aatccaggtc accctcctcc
     3901 tcagactgca ccagtcgaag ctccacgccg gtatcgcgga actgacttga tcgattcggc
     3961 tgcacggtca atggaacgtt cggcggctga accgacggtt gtgcgaccag gagacggttg
     4021 aacacaccgt taaccaggtc gagcacctgc acgtggctcc ggaaattggt ggcgagctcg
     4081 caacgcaaag ccccgccttg ttccagccgc tcgacgacat ggtcgaaggc ctcaatgtca
     4141 gcccgccgaa acgcgtagat cgattgttta ggatcgccga cgatgaacag ctttcccgat
     4201 tcgagctcga tcgtccgcca ggatgtagat tgttcaccta accgctccgc caggtacaac
     4261 acgatttcat attgcaccgg atcggtatcc tgaaattcat cgaccaatag agcgcggtag
     4321 tcccgtttca gctgctcgcg aatcatcgga tagtcccgta gcagactgcg cgccttgccc
     4381 accaatccgt caaaggtcag ccagcccgta tccaggaacg attttctgac tgcgcggata
     4441 aacggcgcca atacctccaa cagttgcccg agcagccctt gatccacctt gagcaaacga
     4501 ttggcgatcc gctggagtga ttcggcctct tcgaagtctt cttcgaccca tccgttcggg
     4561 gcctttccaa gttctttggc cagcaactcg tgagagtcag gtggaagggc gtggattcca
     4621 tccaagccat cgtcgagcag caggtcgaac agatcaacaa tcgtggccag catcgactcg
     4681 atcttgcgcc gtttcggacc gtcatagcgg gccagtagac gtgcggcttg cgccttccgc
     4741 tcggcgaacc agtcgcgcag cacctggttg agaccgttct ccgtgatctg cgccctcagg
     4801 ccatcgagat cgatcagatc gctatgcagg ctataggcca attgccgcag atcttccaaa
     4861 ctgaaggccg ccaacagcag ccgccaacgc ccatgatcgg atcccgacag acccaactca
     4921 cggtccaacc agagctccca ttccgcggtg aagtgctctt cgaagcgaga accatcctca
     4981 tcggtttgaa acgtcggact gaccccggct tcaatgggat agagacgcag caggtgggcg
     5041 gcaaaactat gcaaggtccc gatttgcgcc ttttcgatat cgcgcagcgc cgcttcagcg
     5101 cgcgccacaa tttcgtccgt cgtccagccg aaacgcaggc gtaaatctgc gatcgaaacc
     5161 gtcccgctcc ctgtggccgg attgtcccgc ccttcgagac tcaccaagga tcgcagccgc
     5221 tcgcgcaacc gcagcttcat ctccgtagcc gccttgttgg taaaggtcag ggctacgatg
     5281 cgtgagagat ccagcgggtc ggcccggcgc atgagcagat acaacagccg gttcaccagc
     5341 agggtggtct tccccgttcc cgctccggcg atcaccacga cgttgcggtc gaaggtggtg
     5401 gccgccgctt cccgcgccgc ctggtccggg attgtcgatt gctcagtcac gtgccacctt
     5461 ctcaaagcgc agcgcgcgta atgcaccggc cagggaggaa cggcgagtcc gccacaagct
     5521 gggctgatga gccttccggc aggcggtcgc aaactcacaa tggtcgcagt gggccgtatc
     5581 cggtacgatg aaatgtctcc cgctccgaat gccatcaacc aggagttgca ccgttcggct
     5641 caacatcgga ccggatggac cttgccagca agaggccgga aacgatgcac gctcaaccat
     5701 cggcatgccc tgtggcagga gatacaaaaa ctccacctgt tcaggctgag ggccgtgagg
     5761 ctgttcgcca ggcccatcga cggccatcag cgtgtacagc gcaggctgta accgctgcgc
     5821 acgcaccgcc gcctgcagaa gctggcgatc cttcgcgtct atccggtcat tcgcgcgata
     5881 tttgtagtcg atcacccgga gcgcccccga caccggatga cggtctagcc gatcccatcg
     5941 tccacgcagg ggaatggctt cccctgcggt gccatcaggg aggacaccct ttgcatcgat
     6001 ttcgcactcg accggaataa accctgacgc tgcggcggcc tcgcaatcga acctgaccat
     6061 cgcttcgacc agatgtcgaa ccgtgtcctg ggccaactgc caggtaaggg catatccggt
     6121 cccgtgggtc atcgcataga cggtgaacgc ctgcgtgacg gcccgagtga cttccgcggt
     6181 gacaaccgca gcggacgatg cagccgtccc ccacccttgg cgaatcagcg ccagataaca
     6241 gagccgcagc gcatcatggc acagttgccc catagcgggc ggtgacagct ccatcgagac
     6301 cgtctgccgt accgcgtcga ggtccagcac ctgggccgag aaatactgaa aagggcaacg
     6361 cgcataggtt tcaagcgcag tcggcgaaat cccgcgttcg cggatccggt tccaatggga
     6421 ggtggcgtcc gtcaacaggc catcgtaggc actcaaggcc ggcacgccgc tttcgatcag
     6481 ggcttgcgca gcgagaccat gagcaaacag ctcaccctca cgcccccctg cattcaacag
     6541 ggaagccgca tcgcgtccgt gcaagacgct actcaccgta tgttcttccc gcgtcaggag
     6601 cggcggaatg aataaaggca acgccgtccg atccttccat cgccgcggca cagcgaacag
     6661 agactgtgcg tccgcagcat gcggcaccac tcccatgcca tccaaatatc ccgagggggc
     6721 caatggccga ccggcggcat ccgcccgttg atacgacagg tacagacgtt cacgggccga
     6781 ggcgcgcagc agttcgaaca gtaaggcttc ctccccatag ccctgcaatt tttgatcgat
     6841 cttgtagccc agtgtctcac tcaacacgag acgatggctg tcacgtagaa atccgtcttc
     6901 atgaatgtaa cggggaaaca ttttttcgtt catgccgacg acgaacagcg cgcggaagcc
     6961 aaggcccctc gccgccatgg cgtcaagcac ctgcacaccc tgttgggacg gagacgtcaa
     7021 ggcgtaggta gtccgctcca gaattccctc gaacgtgtcc gtccattcct gccacgtgat
     7081 gcggacatcg agccggtcca actcacggag ttgcgcaaga gccccgacca gtgcttcggt
     7141 gacatcggtt atgccttgag actgcgtagc cgcgtcgatg gaatcagaca tatcaagggg
     7201 aatggaaaga tgatcctcgg ccagcgcgca gaacgcatcg gttaagccgc catatcctcc
     7261 ggattccggt agccccgtga ctccatctat caagggggag acgcagtccc agagcaatcg
     7321 cagttgaggc ccatcaatcg agagggcatc gagcggatct tcggaagact catcgccggt
     7381 accagcccag gcctcaaggc gtcccagttg cgccaggcgc cgccactcct cttcgccctt
     7441 cgtaattccc aatgcatgga cggctaaccg ccagaggtcc ggtcgcggcg gttcgcctcc
     7501 ctgcggagcc gtcaggcggc gattccacgg cgaggtgatg acgtcgagca tcgccgaacg
     7561 atgcaaaccg ttgcccttga gcctggccag atggagcaat gttttcatcg ccggctctcg
     7621 caacaacgga acggcggccg tcgacgtaaa gggaatgcga tgttgatcga aaagacgcac
     7681 gagtgtcgat tgatacggca cgagggtccg tcccacgacg ccgatctcct caaatcgata
     7741 tccgtgggtt tcgaccaacg tgaggatctg cttgcagacc agcgtcaatt catcgtcgat
     7801 cccagccgca tttcggactt caaccgatac attggctctt gtcccatcag atccagatgg
     7861 ctccggtgag tccagcatcg atgcgtcact ggttcccgcc atcggataga gatgccgttc
     7921 aaggaactgt cgcgcaaatc cataggcggg ctgagcatcg acaggaaaat acaccgtgac
     7981 cggaagagtt gcagcgaggg attccaataa cgtgagctga gtttgcgtga gatcgtacga
     8041 cccgtagtac cagagttcat ggaggccgcg aaggaatggc gaagtaggca caaacggagt
     8101 gaccagcgaa gccaagtcgt ccggtgaccc gacgcctaag gccgcgctcc cctctcgaac
     8161 ggctgcgtag agggtaaata atcctttgag cttttccccc tcctccgggg caaactgccc
     8221 ttcctctacc gcgcgtacgg ccacagccga atccacggtg gcatccttga gatcgcgaag
     8281 actcgcccaa agtgcaggcc aggcgccgtg cggaagctga gagagacgga gggactccgt
     8341 gtgtggcatc cggcgctgcc cgatatgccg caggagatgc tcgaaaaaga catctgacac
     8401 cagatccatc tgccgtcccg gggctgcgcc ttcagcggtg cgccgttcac gatccacatg
     8461 cagagccagc tggtgaaacg agaggatgtg cacattcagg agggagagct tctcttgcag
     8521 gatcaagaga cgcttgagtg agcgacgcaa ctgatcggaa ggaacaatga tggccagcga
     8581 agccagccgg tcttttgatt tcagagcacg gagatcttga acgaggcgcg tttcgagcgt
     8641 gggatggaat gggccggtta cgaggcggag catctaacgg aagtgagcca cggtgaatgg
     8701 gacatggtca acggcttgcc tgttcgctga tcgtattcat tcgatatcga ccggtgaacg
     8761 ctccggccac taaccggttt ccggacccag gagttcaccg ttgcaatcaa cacaggccca
     8821 atcgccgtcg ataaacttca tcggatcgcc gcaggtgccg cactccggcg gcggcccctt
     8881 gtcgatcatg ggaagaatcg gcagctcgaa atcatcgtca tcaggaacgg tcatatatct
     8941 ccgatctcac gcgccctgcg gtttcatctt cagctgcagc gccttttcgg cgctttggcg
     9001 aggcggcacg ccgacgaacg tcaaccggcc atcgataatg gtcgcgggca ctgcccggac
     9061 tgaatgtcga tttgccaatt cctggccgtc cggggtcgtg atatcgacct cgcgatagct
     9121 aaagctatac tttaccctga gttccttcca caggcgtttc gccgaaggac aggctccgca
     9181 ggtcggtgaa tgcaataagg tgatattcgg catggtcaaa cccctctcct taacgtgagc
     9241 ggatcttaac acccgcgccg aggaccgcgc aaccgagcgc gcctgacaat cgagaccgag
     9301 ctgtatatac tgagccaaag cggatcggca ctcagcatcc acccgtcatc catcctaatt
     9361 gaggcgtcca tggctcagca tcccctcgcc ggccaaccgg cacaaccgga acagttcgtt
     9421 gatctcgccc gactcgagca ggcctattac caggagacgc cggatccggc cgatgttcaa
     9481 cagcgcgtca gcttcggcac cagcggtcac cgcggctcat ccttgaaacg aacgttcaat
     9541 gaagcgcaca ttctcgcgat cactcaggcg atctgtgagt accggatcaa agccggcacg
     9601 accggccctc tcttcgtcgg caaagatacc catgcgctct ccgctcccgc ccagcgcagc
     9661 gcgctggagg tgctggccgc gaacggtgtc gaggttcgcc tggataaaga cgacggctac
     9721 acgccgaccc cggtcatgtc ccatgcgatc attcagacca accgcgaccg aacctctggc
     9781 ttcgccgatg gcatcatcgt cacgccatcg cataatccac ccgaggacgg cggattcaaa
     9841 tacaatccgc cccacggcgg ccccgccgac accgacgtga cgaaagccat tgagaaccgc
     9901 gccaatgaac tcctggccgc gaaccttcag ggcgtgaaac gcatgcccta cgagcaggcc
     9961 attcgagcgg tctctaccac gcgctacgat ttcgcgggga cctacatcgc agacctcgcg
    10021 caggtcgttg acctcgatcg catcaaggcg gccaatctgc gcctgggcgt cgatccgctg
    10081 ggtggtgccg ccgtggcgta ctggcgtccc ctggcggagc gatatggatt gaatctggag
    10141 atcgtcaacg catcggtcga tccgaccttt cgcttcatga cgctggattg ggacgggaag
    10201 atccgtatgg actgctcctc accctatgcc atggcctcgc tcatcactct caagaaccgg
    10261 ttcgacctcg ctttcggcaa cgacaccgac acggatcgcc acggcatcat cacaccaggc
    10321 gccggcttga tgaaccctaa ccattacctc gccgcggcga tctcctatct gttcacgcat
    10381 cgccccgggt ggaaaagtag tgccgcagtc gggaagaccg tcgtcagcag cagtatcatc
    10441 gaccgtctgg tccgctcatt aaaacgccgg ctggttgaag tgccagtggg atttaaatgg
    10501 ttcgtgccgg ggctcagcga cggctctttg ggattcggcg gggaagaaag tgcaggcgcg
    10561 gcatttcttc gtcgtgacgg caccacctgg gtgaccgata aggacggcat catcatgaac
    10621 ctgcttgctg cggagatgct ggcggtcacc ggaaaagatc cggcgcagtt gtacggcgaa
    10681 ttgacggccc aattgggcga gccggtctac gagcgcatcg atgccccagc cacgagagcg
    10741 caaaaggccg cactgcaaaa attctcaccg cagcaggtcc ggagtaagga actcgcgggg
    10801 gaacccatca tcgccatgtt gacagaagcg ccgggaaaca aggcctcgat cggcggcctt
    10861 aaagtcacca ccgccaacgg ctggtttgcc gcccgcccca gcgggacgga ggatgtgtac
    10921 aaactctatg cggagagttt caagggacag gcccatctcc tccagattca ggaggatgcg
    10981 caggccttga tcaaacaagt cttctcgagt gccggcgcgt gacagcctac tgaatcagcc
    11041 cctgccgggc gatcaggctc ttggcaagac gccgggcaag gcaggagatg ctaactttta
    11101 ccgttcatgt actatgtgcc ggactgcctt gcggagcagc ccgtgagggt ctggcatgat
    11161 gttggcgaga atagttcgaa gacgcgctat acttacagag tatgccgacg ctctcacgtg
    11221 catggtgtag cctggccctc gcctccgcac tcaccgcttg cgccgctccg tcgtcacaag
    11281 gactgcggca ggcggtcact cccattcccg attgttgccg tgccagccac agcgacactc
    11341 gtcaacaggc gatcgttcgc acagccgtca acctggtcgg cgccacgacg atcgaaagcc
    11401 agggccgtcg catcagttac gattgcgccg gagtgacacg cgccatctat ctcgcccacg
    11461 ggattgatct ctactcagac ggctcagccg acggcaggac caacggcgtg ggcctcattt
    11521 acaatcatgt ccggaaaaac ggtcgactcc atcgcggacc ggtggtacag gccggagacc
    11581 tggtgttctt cgacaatacc tgggacttca acggggatgg cctggtcaac gatcccctga
    11641 cccatgtcgg catcgtggaa caggtggagc aggacgggac catcgtcttc atcagccggg
    11701 tggccggcgc catcgagcga taccggatga atgtggcgca tccgcatatt catcgcactg
    11761 ccgacggtcg tctgctcaat gattatatgc gacgaaaaca ctggcgggat ggagagcaca
    11821 cggcctacct gaccggcgag ttgtttgccg ccttcggtac gagagtgata gactctccct
    11881 caataccacc tgcaccaagg taagagcctt gtccgccatc cagacgtcac cgcaccgttg
    11941 tcccgagtgt caccaggcca ccgcctggaa ggacaatccc tggcgcccgt tttgttccga
    12001 gcgctgccaa ctgatcgatt tgggagcctg ggcaggagag cagtaccgcg tgccagggcc
    12061 tagcctgagc gtgggtggga cggaatcgtt ttcgccggac agtgagtagc cgaggcagcc
    12121 tacctcattc gcagcgacag ttcgccttgt aatccataca catcggacca aagctccgct
    12181 cgcaatcgca actgcacttc ggcgttttat cggctgggca ggtgatctgg caggtttgct
    12241 tgatgcccat gccgcaggac acgatctcac aggaattaga tccctcggcc atcaggagcg
    12301 gcaatgacgg ttccaccgga gcgggcgtct gctgcaatgc ccctgggcgg ggtgccggag
    12361 actgcgtgac ggacgaggga ttcgacgcgc caccttcttc tgcctggatc cgtgacgtca
    12421 acgcgggcaa caatgtgacc gacaccaata gcgccaccgc aacaccggtt cgtagtagtt
    12481 ccctcagcat gacgtcacct tcctttcaga gggccgcagg ataggaagaa ttccgcgcga
    12541 aatcaagtcc cgcccactca cgtcaggcag tccaccaccg gataacgccc gccaccgaat
    12601 gatacagccg gcaggcccca ccactgttca atcacggtcg catacagact gcggtagtcg
    12661 agggtatatt tcagatcgcc gccttccagg tccgtcagcg aacacggtgc gccatacaag
    12721 ccgcccctga cgcgcccgcc catgaagaga tgcggggctg cggtgccatg atcagtaccg
    12781 tggctggcat tctccccgac ccggcggccg aactcggaat aggtcatcac caacacctga
    12841 ctccagagtc ctcgttgttc cattgccgcg cggaacgcca tcaatccttg ggtcagctct
    12901 tccaacagcc ggtgatgggt agacagttga ccggcgtggg tatcaaaact gccgtgagtg
    12961 accttgatga ccgcaaccgg cagcttcgca gcgatcagcc gggccgcgac ttccagttgt
    13021 tttccgaatt tatgagtcgg gaagtccatc gcgaacggcg gcgcctcctg aacacgttgc
    13081 tgcacatcga tggccgcttg ggagatctcc cgccgaacct ccagaatatg ggcgagtgcg
    13141 cgattcgtcg tagacgcaga cagcggtttg agcgcaccgg cctgacggaa taactgtgca
    13201 ggatcatgca gcgccatcgt gcgcgtcgtg cctccgctca gggggcccgc gtctcccctg
    13261 cccaacagaa cgccttcggc cgtaaatccg gccggcaacg ggtactgtgc aaacaggcca
    13321 gacagccatc ccttgtctcg cacctcttcg ctatccgagg ctgtgtccca gatttcaatg
    13381 gaacgaaaat gggatcgatt cggcttggga tacccgaccc cctggagcca cgccagctcc
    13441 ttgcgttccc acagcggcat caacggcgac agggctggat ggagaccaag tttgggagtg
    13501 agctgtcgaa cctgatcacg aggaattgcc agttgcggtc tcgcccggta gtacgccgcg
    13561 tcatcgtatg ggatgaccgt gttgagaccg tcatttcccc cctgcagctc caccagcagc
    13621 agaatacgct cacgcttgtg cgaccgctcc gagaaatcac tcgtacccgc cgcccggagc
    13681 ggcgacgacc aacccgggcg caaccacaga agcgggagcg ccatggcggc ttgcaagaat
    13741 gcgcgtcgat ggacgctcct cgttgtcatg gctgcctcat ttcaattggt agaccgggtc
    13801 cagcaccagg tgatgcacgg cctgcccgcg atcatccccc gcagtcagcg gctgcaccgg
    13861 cgcaaaggga agcagcgtcg cctgggctac ctcggttgtc tccatcgcga gccacatcgc
    13921 cccctgagcc tgcttcatgt ccgtcatgcc gtgcaggtgg cctgtctcat ggccacggat
    13981 tgcccgctgc agcagctgcg tgcgactcaa cagggttgat gtggtaatcc atcgcaggcc
    14041 gcccggccac cccttgacgt tcggagggtc gaagagatct tgtcccagcc tgcggccata
    14101 cacgacctgc aacgtcacat catcaaaggg aaggtcgaac aatctcatcg tccccacgat
    14161 gagctcaacc ggagacttga tcaggacgcc tcgattctcc ggcgcccaaa atgcctcggt
    14221 caggaacagt tccttcaaca ggtcgactgt ctcatacttg ttgcgtctga atgagtcggc
    14281 gagacgttcc actgcgaccg catcaggctg ctcggaaata aactcacgcc acagtttcct
    14341 gacaagatag cgggccacat gaggctgctc cagtgtcagc gagagaatat cgtctccatc
    14401 aaaatgccct ttcctcccga atacctcctt ttgcccctcg tcatggtgtc gcagatccat
    14461 gcgaaacgca ccggtatggt ggtcgaggtg ccagccggta aacgcgcgtg cagcctgctt
    14521 gatgtcctgc tcggtgtagt gtccttcacc aagggtaaac agttcgaaca attcccgggc
    14581 aaaattctca ttgggatgct cccggcgatt cgtgtgtgtg tcgagataca gaagcatggc
    14641 gggattcttc gcgatggact gcagcaaggt gcgaaacgat cccaacgcat ggagccgcaa
    14701 cagcacgttc tgttgataca gaaatgccgg ccatcggacc ttatgtaagc tggacgtgaa
    14761 atggttgtgc cagaacaggg tcatgcgctc caagagcgga tggcgcgtcg tgatgagttc
    14821 gcggtaccac caggccttga gttcatggcc ctgtaccttg agctgttcgt gaaaggcctt
    14881 ccgctcgcgg tcagtccaga acttccgcag cctggggagc ggaggaagtt gatgaatcca
    14941 agcgggaggc ggagtagagg ccttatgcgt gggaatggtc aggagctgtt tgacggcgtc
    15001 cggccggtcg aatgccatga acgcgcgaat ctcttccggc gttccaccga agccggtgcg
    15061 agagagcaga tgacgagcct catcgaaggt cagccccatg gaagatgctc tccacggtca
    15121 atctgtcgct ctttacgaca acgccggagt gctcattcgg ttgaccggta ccattccgtc
    15181 cactgcgcct cgtaacattc cgagtagaga acggctccgt cgattttgta ggatcggcca
    15241 tgcgttttgc gctcgcatcc ttgcttatcg tgctcacaat cggcttcggt accggctgca
    15301 gcacgccctc cacgtccaaa ctgacggggg ccgtgcggta tttcaacatc cggtcggaaa
    15361 tcgcgccgcg ccatttgatc gctcagatcg gcgatgaaat ccggtggcaa aacctgaacc
    15421 cacaccccgt caccttacgg atgctggaag acagcgaccc ggcgttgctg gcatgcgaca
    15481 agggattcaa gcagttcggg gtcattcagg acacggtcac gatcgcgcca ttacaatcgg
    15541 tcagcctctg tttcctgaag cccggcacca tccgcttcaa cgtctggctc gacgccgaca
    15601 atccccaggg cgccatgacg ccgaccggta ctatccgcgt tgagccttcg ccagtcaggc
    15661 gaaattcttg aactccttcg cctaacgcct cagccccaga actgttcggc ctttccacat
    15721 tacattcgaa ggcatcacga catcgcgaat gctcgacgaa cgcgagcctc acgacgtacc
    15781 agcgctgcac agagctgctc agcagggaat attgatggca ggatcgcaga agagcggagg
    15841 gatcacggtc ggtcgcatgt agcgcgagga gggatcctgc gaccccatcg gcgcagtcag
    15901 cgtcgggccg ctcgtcatac ccggcaaccc ttggccgatc gtcccaaacg atttgccctg
    15961 gctggacggg atagtgttac tttgtggcgc gctggctccc gatgccgtgg atgaaatcgc
    16021 cgactgctgc gcgaggggat tgggaatcgg aatggtgccc ggagcctgca gatttgatcc
    16081 cggctgattc tgttggttca actggttgga agacggcggt aacgattgcg ccgtaacgag
    16141 agcgggcgcc aacagcagca ggcatacgct gatacgcagt actgatcgct gaatcgacca
    16201 cgttcgaccg ccttgctgca tcacatcgcg ctcccttcgc acactaatcc aaccaatctt
    16261 tctccttcag cttgcgcgga aggtacttct tggtcagata ttggaaatcc ttattcgcca
    16321 acgcgtccaa ttcgtacttg aggccactcg tcagcatgcg tttgatttcc tgctgccagg
    16381 ccggcttctg gaaccattgg tagttcagaa gttccttggc gcgggtcacg tctttttcat
    16441 tgagcttgat cccgacattg cgcggcaact cgtaccgctc aggatcggca ctggacagtc
    16501 cgatgaattt cgctttgggg atggccatgc gctggctttc gaacgcgaga ttgatcgacc
    16561 cctgtttcac caccgagtag atatagtaac cccagggatc gttatcgact aacacgtaca
    16621 ccgggagctt atgttcttca tgaagccgtc gcgccaggcg gcgcaccccg cggggtggct
    16681 gcccgttccc cgtcagcagc acacagttgt aacgccgcca gaatttatct tccgataagc
    16741 ggttccactg cgtgcccttc tccaccaaca acaggaaatc ggctgtgcag cggcggatct
    16801 ccaaatactc cggctccacg atcgagggga cggaatagcc gcccttccct aacttagcgc
    16861 aatccacccg atcgccgtca tcgccgaaga caaccggccc cacgatgctt ccgctgtttt
    16921 ccgctcgcac atgtaactct tcacgcaacg cgatgagcga cacttccaga tcctcgatga
    16981 tgggatcgga ctcatcctgc gtatcgaatg tgttctcatg cgaatcttga atcgtatgtt
    17041 tcgtgcggta gtagatttcc ctcagcgacg tagtcaaatc ggcctgctgc agctcagcca
    17101 atgcgtccgc caccagcatc gtctgcatga acttcttggc cataccgacg ttgaaaaacg
    17161 tccgggcttg ttttttctca cccatctcga tcaaaccctt acgaggattg aacgagacgt
    17221 tggacagggc ccgaatggga atctcaaacg tcggatcttt ggatcgttgt gccgcggaaa
    17281 tcacgacatc ggccatacca acgagctttt ttgccactgc accgttcttt ttcggtttcg
    17341 cctgtgccat aagctgtcct atttcctttt gcgagtgcct gtcgccgtct tagagggctt
    17401 agagggtgtc ttgcgagccg gcgcgtgctt cccatcagct gcttcagcaa acagctcctg
    17461 ctgcccggct ttgcgcttcg cattcgaggc tggccgactc tttttcgatg taggcgcggt
    17521 cgcgaccgct cgcctgcctt tcttgggaac ttttgccgga gcagcggatt caggagaggc
    17581 tgcactcacc ggaccatccg caggctcggg aacattctca gcgacctcac gctcgccttc
    17641 gatgccttcc gccgtgacga taatggaatg gggcagacct tcaggaccgg cgccggtttt
    17701 cccgagcgct tcatccgtct tgagaccacc cgttcgtgaa ctggcgatct tgtgtaactg
    17761 ttctttcaat ttttcagtgg gcaggtttcc gcccttcaaa cgattgcacg cagccaccac
    17821 ctcttcgatg tacaactcga aaatattgcg gcgccggaat tcgctggcgg cacgttcgcg
    17881 cctgcgcagg aaaattccca accgccgccc ggcttcgcgc agggccagcg tgatttcctt
    17941 ctgaatctca tcgtagtcgg cgatggcttc tttcgactca ctggtgaacg gcacccaaac
    18001 ggaagccata tgcacaaata tcaccatcgg tcccgcaggc aacgcgccac gggattgact
    18061 gacaccataa ttgcgccaag tggtactgag tacggccttg aaagtcgcgc aggcagattg
    18121 ttgatacaac aacggcaccc gattggcgta gcggatcacc cgcgcctgct cggtatcatt
    18181 cccctggtct tccccttccg ccaggggaac tgcggcgtgc tgcggcttcg aaggttcttc
    18241 cggccctttc ccgaacgcca gccccgcttc gatgacaaag ggatacccgc gatacacggc
    18301 cggcggccga ctgaccgccg tgtaaaactc gcccttgatc tgcttataga ggcccgaaag
    18361 aatcgccttt tcgccgatcg gggaaatgca attggtcggc ggcgccatga tcttggtcgt
    18421 ttggatggtt ttgtagagcg tctccgccgc ctggggtttg acatcgcgcg gtttggtcgt
    18481 gggcgacagt tttgcggctt tgcagatctc ctcggccagc gcgggagaca cccggcagaa
    18541 gtcgctggct aaaaacgatg cgaccgtgtg gctcttcgta tcctgcaaca tcttgagcaa
    18601 cgcaccgaat tcaatgccgt agggatgcgg cttgatctcc ttcggcgacg gcggcaactc
    18661 atgataggtg cgcgggtaac ttcgctgctc tccttccggc gtgtgatacg tcagtcgcac
    18721 atgcggattg gcgatcgatg tctgttccag ccattcatcc acggaggcgc gccccttttg
    18781 ataccgtcct tcgacttcca gcaccacctc ggtaccttgc ggttgcgacc actcgatctg
    18841 tttattctca tgcaccagcg gttcattctt cttggtgtcg atttgcactt cgaagtaatg
    18901 ggccgcagct cgcggtccgg tgcgggagat caccttcacc ggcttgccgg tcgtgagctg
    18961 gccgtacatg ccggccgcgg aaatgccgat gccctgctgt ccccggctca tccgcatgcg
    19021 gtgaaatttg gagccataga gcaacttcgc aaacacacgc ggaatctgct gccggacaat
    19081 gcccggcccg ttgtcggtca cggtgatgcg aaagcgggtg gcctggcttg gtgcgaccgg
    19141 agccggtccg ttcgcgacca cttccagctt caccgtcacg tcaggaagga tgcccgcttc
    19201 ttcacaggca tcaagcgagt tgtcgacggc ttctttcacg cacgtcagca acgccttccg
    19261 cggattgtcg aatcccagca ggtgccgatt cttcgtgaag aattcggaca cggagatctc
    19321 acgctgccgc gcgcccattt ccactgccgt caccgcgggc gctgattttt gtgcagcgcc
    19381 ggatttgttt cgtgagacgg ccggttcgga ttcagtggat tcggagggag gcgcggatgt
    19441 ggaagactcg gcacgtggcg atgatctctt ggtggccatt caacctctca agcaagggat
    19501 tcagagaatt ggcgcggcgc agcgacaggc gcgatacgta tcctcctcgt cgtttacagg
    19561 gaacgctgcg gagagtcaag ccttgaggag attccggtcg ccgcacctga tgcgcaacct
    19621 gtcgcggaag tgacacgact cagtgttatg gaaccgggct tgcgaagaga aggcaggttt
    19681 gggtgggagg aggacgcacc taccgaggag aggtagatac ggcggagatc tc
//
LOCUS       DAOQEW010000055        19701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_696_length_19701_cov_7.208083, whole genome shotgun sequence.
ACCESSION   DAOQEW010000055 DAOQEW010000000
VERSION     DAOQEW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_19701_cov_7.208083"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLS35_08045"
     CDS             complement(<1..665)
                     /locus_tag="PLS35_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18649.1"
                     /translation="MPVLKKSALDGYVKARFGSTAELVAYGPIGKETQGARYKQYGYG
                     APIRLTFRTNGNKTRQVVLGTMSPGPFGHEHPADRAQAMLMDYDCYSRLPRHITALDV
                     GAFTARGELMSVAAAKEFFLLTEWSEGDTYHKDLERLADATRPTPLDRKRTQALADYL
                     ATIHRVKRRDPQLYRRRLRELLGHGECIMGLTDSYPHRYGFITEELLQRIEVACNAWR
                     WRLR"
     gene            950..1495
                     /locus_tag="PLS35_08050"
     CDS             950..1495
                     /locus_tag="PLS35_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNI18650.1"
                     /translation="MNLEVESRNIEMTPRWKTEIEERMAALQRGHDDIIHGRVTLTKN
                     RHHKKLDNVAEALVLVTVPSRQTITSRKEDKTFEEAIRSAFDAVAIELKKFREKRADK
                     VIRVEPLPQRRGVVSKVFPEQGYGFILMDGGGEVYFHKNAVKGMAFADLEDGVEVLFE
                     SEPGEKGLHATIVQPPHLLKL"
     gene            1506..3917
                     /locus_tag="PLS35_08055"
     CDS             1506..3917
                     /locus_tag="PLS35_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18651.1"
                     /translation="MLDNHRLPASLLAPTVDPARLGFADTSQVQPLEETIGQERAVEA
                     LEFGLQMKSPGFNIFVSGPVGTGKGTLVRQMVQRLAQTAPPPPDWCFVHNFQDASRPS
                     CLSFSAGQGASFKRDMSTFIEGLRRDIPTTFEGKKYLDAKAKIIEETEDKKKNLFHDL
                     TILCHQRGFVFEETAGGFALVPIKDDRPMTGKEMEELPESIQEELTARRQSLESDIRE
                     FHVRMHGLEREAEQALHHLDHQIVANVMKGAYDALLQTYQSSQLVVTYLQRVHDDIVH
                     QYRDFLPHTGPMLAIPGLEQSRRPDLTRFAVNLIVSREAAGGAPVIDESHPTYSNLIG
                     KIERRAHLGVMYTDFTEIRPGSMLQANGGYLILQALDLMRQPFSWEALKRVIKTGAVT
                     IEDPSEFFGFATAGLRPEPIPVSVKVILVGTAGIYHLLQAYEEDFSKLFKVKADFDVE
                     VPHDEHQERQYARFVSRLCREEGLPHFGADAVAEVIRQGFRFADRHDRLSLRFSLVSD
                     LIREAGYWAQKSGHVLVTRADVESALAHQRRRADLPEQWLQGEIAEGTLMVDLQGEVI
                     GQVNGLSVYELGDYCFGRPTRITARTYVGTKGVIDIQREVDLAGEIHSKGVMTLAGFL
                     AGKFAGMQPFALSATLTFEQTYSEVEGDSAAVAELAAVLSSLADLPVRQYLAVTGSVN
                     QLGEIQPIGGVNEKIEGFFESCKRRGLTGKQGVIIPARNIKHLALNREVVAAVEDGRF
                     AVYGVDTADQALELLIGVPAGERGAEGTYPPVSIYGRTAARLEEMAQIVATWGEAEED
                     NRVKD"
     gene            3951..4115
                     /locus_tag="PLS35_08060"
     CDS             3951..4115
                     /locus_tag="PLS35_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNI18652.1"
                     /translation="MPMYDYTCLDCGKESLIVVTLKQHEKGEVQCPACGSTKLQQHFS
                     SFIAHTTKKS"
     gene            4133..4516
                     /locus_tag="PLS35_08065"
     CDS             4133..4516
                     /locus_tag="PLS35_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI18653.1"
                     /translation="MRTFTTLSIRHLILATAAGLMLAPVVDDAHAQEFPPDAAKGKAV
                     YERHCLACHGAGGRGDGPDAAGLTVPPANFHRFKSFLKSDEELLRTIEHGVVFSPMHA
                     WQGQLTETERQDALAYIRLLVQQGK"
     gene            4568..5149
                     /locus_tag="PLS35_08070"
     CDS             4568..5149
                     /locus_tag="PLS35_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNI18654.1"
                     /translation="MTNLTSHDALVVVDVQRDFLPGGALGIQDGDKIVPILAGYMAQF
                     HARGLPIFITRDWHPPDHCSFRDQGGPWPVHCIAGSPGSLPPPNFQPPVSAVTIYKAI
                     DQDKEAYSTLQGTPLDRHLRALKVRRLFIGGLATDYCVLNTVKDALGLGYDVCVLMDG
                     IKAVNLHPDDGRNAEEAMLHLGAVPVRLESLAA"
     gene            5146..6480
                     /locus_tag="PLS35_08075"
     CDS             5146..6480
                     /locus_tag="PLS35_08075"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNI18655.1"
                     /translation="MTPSTTSLLTDLYELTMTQAYLAHNMTGEAVFEFFVRKLPTRRN
                     FFMAAGLEQVLSFLEQFHFSEGDLAWLEERGRFPPETLIAFRAMRFTGDVHAMPEGTV
                     CFPHEPILRITAPLPQAQLVETRVMNLLNFQTMVASKACRCILAASGKPLIDFGLRRS
                     HGAEAGLLAARASYLAGFAGTSNVLAGAQFAIPTYGTMAHSFVQAHRDETTAFLHIAQ
                     SQPHHVVLLIDTYDTEAAAERVVMLAPELKRQGIAIHGVRLDSGDLGAHARKVRHILD
                     RGGLEETTILASGNLDEERIEALVTSGAPIDRFAVGTAMTTSADAPYLDCAYKLQEYA
                     GRPCRKRSEGKATWPGRKQVYRRLGPDGRLAQDTITVVGDTLPGRPLLRQVMEGGRRL
                     APSPSILEIRDRTRLALSELPDTLGQLDQAEPYEVRISGALERLAQQVDRYT"
     gene            6546..7973
                     /locus_tag="PLS35_08080"
     CDS             6546..7973
                     /locus_tag="PLS35_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNI18656.1"
                     /translation="MNQHSLSAAFVAGLVVTTLCSIPDGALAAAKSASKSALAVACES
                     QYQRTRPAPKVVDKVLQAHARWLEDRESADGRRANLCRTDLRQLRLTTASLERANLEG
                     AILKGANLRSANLVQAHLKGADLSHAILDDANLEGADLRKGLLAKAHLNRISADEAAF
                     YGANLQGAFFREALLERAHFEDADLQLADLSGASLLDGYFYGANLSKANLTDADLAGT
                     DLRRTNLRQATLRRANLQGALLDSATLDGTSLVEADLESAYLDDASLVQADLHEASLR
                     GADFRYARLTNANLQRANLENANIEGANLAKARLNSATMTMSVLYKANLTSANLHGAR
                     LHHAVLIDAHLSRADLQKADLTEVYAPKAHLQQARLAEANLELANLVSADLSEADMSH
                     SIMVQTNLQEANLRGTNLTAADLTGAQLNNADLQQANFRGANLSGALGLVQAQLDQAC
                     LDEATQIPTDLQRPKACPPPRQKRK"
     gene            8147..8677
                     /locus_tag="PLS35_08085"
     CDS             8147..8677
                     /locus_tag="PLS35_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18657.1"
                     /translation="MNLRRKTMFLLAGLAALAVTLLLIWCPPALCRVLNIYTFPLSNL
                     SDFRQYVADEEAFYSLHHTRAVRIDCAALRDEVVTVLKIGHLVLETNTPGDTQRERRP
                     GTIEAWHQGRLLHLTNTALASKDEQQAWATSVRAVVTLQQQQDLAFLSRLFGAVVLHV
                     SHRQIPIVLFSDRPCL"
     gene            8925..9338
                     /locus_tag="PLS35_08090"
     CDS             8925..9338
                     /locus_tag="PLS35_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18658.1"
                     /translation="MKWTHCLPMLLALCLALPTASPAGSPLPAEPGDDFLIEQTFDSV
                     VGLLFRDYSLQGNGQVDYRTARHILGISYDDPASAEPDVALHPLFYWYDRNQDGQWEL
                     WIDREEEGHLVDVTRYDWRQGDDLLTLYPNLRNPT"
     gene            9426..10652
                     /locus_tag="PLS35_08095"
     CDS             9426..10652
                     /locus_tag="PLS35_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI18659.1"
                     /translation="MPSSHSRHKLPSGIWILGFVSLLMDISSEMIHSLLPLFMVTTLG
                     AGTIAVGLVEGLAESLALVVKIFSGTLSDYLGKRKGLALCGYTIGALTKPLFAWAPDI
                     GTLLTARLLDRLGKGIRGAPRDALVADIAPPQLRGAAFGLRQSLDTVGAFLGPLLATG
                     FMLLWADNFRAVFWVAVIPGMLAVALLMVGIQEPVSSSSSRRANPITRANFRRLSPAF
                     WWVVGIGSIFTLARFSEAFLVLRAQQGGIPLAFIPLVMVAMNLVYASSAYPFGKLSDR
                     INHKTMLTWGLLVLIAADVVLATTNHWGVILGGVALWGIHMGMTQGLLAAMVADQAPT
                     DLRGTAYGCFNLASGLAMLMASIVAGFLWDRLGAAFTFYAGALFSSIAVIGIVVSPGT
                     APAPSAGHSLPPRSGE"
     gene            complement(10683..13613)
                     /locus_tag="PLS35_08100"
     CDS             complement(10683..13613)
                     /locus_tag="PLS35_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18660.1"
                     /translation="MSTIFQHIILNVDDDEGARYAKTKIFERAGYRVVEAATGADTLR
                     LVQEQQPHLVLLDIGLPDISGIEICRLIKKNPATEHMMVLQVSASCVRPQERAMGLDG
                     GADVYLSEPVEASELIGAAKALLRLYDREQESRVLLKELTERERFIKSMVDAAPSTVY
                     LYDLREQRNLYTNARSGATLGYASDELQFVGRETLQSWIHPDDRPLVMAQLAQLPDQA
                     DGQVLEFECRVQHRSKEWRWFLCRNVVFSRDENGQPLQILGTALDITDRKQQDAILRR
                     GKEQQALLLDISRLILEMPVDRAAWTRAIYEKVAPHLDVDLCLNFLLDQKTKGLQLLV
                     GLGVPDAMLAKAQRLDPGQAFCGYVVDAGTSLVADAELISTDPRGKFVREMGARAYAC
                     HPLKASTGEILGTLSFGTSRKERFTEEEIAFFQTLCHLVALAWERHSVSSALGESEMR
                     LKAIVDSALDGIITIDDQGTIHRVNPAVGRLFGYPADALLGRHITLLLPSLYSVEGAD
                     TPGVDQPRDDSGVSGLRREMLGVRMDRSSFPVELAISENHLDDAVTYTLLLRDITERK
                     KVQEQTRQWTIELEQRVSERTEELLLSRTRLRALASDLTLTEQRERQRLATELHDYLA
                     QLLVFGRMKLSQAKRGNLGPATTLVKELDDMLDEALTYTRSLVAQLSPPVLREFGLVV
                     AIKWLAEQMRRHEMTVTVQCEADLAPLREDQAVLIFQSARELLINVSKHAKTSQASIS
                     VWIESDMLHLCVGDEGVGFDLASTTNIAAPMFGLFSIRERMEALGGRMAVESAPGRGT
                     KITLVVPYVGGSTSTDVRSRSDMASSSLSGPEVVSALDALAVSPGPESAHSSRTRVLL
                     VDDHAMLRQGVRTVLEGYADIEIVGEANNGEQALLLAQSLQPKIVIMDINMPGMDGIE
                     ATRRLKLEQPETLVIGLSVHNDWQIEEAMRAAGAVSFLPKDAAIEQLHETIQVAIRAA
                     ARTA"
     gene            complement(13635..15812)
                     /locus_tag="PLS35_08105"
     CDS             complement(13635..15812)
                     /locus_tag="PLS35_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18661.1"
                     /translation="MSYAIINVALKYEDDVVAARQRARQIAGLIGFDAQDQTRIATAV
                     SEIARNAFRYARNGAVEFLIEGQTAPQVLLIRVTDSGPGIADLKLILEGRYQSDTGMG
                     LGIVGAKRLMDQCEIDSAVGKGTRVLLKKVFPSKTRALGAKELSSLVDQLIQTPAQNA
                     QEELRQQNHELLRALDELRLRQDELVRMNQELEDTNRGVVALYAELDEKADHLRRADS
                     MKTRFLSNMSHEFRTPLSSILALSGLLLDRSDGELTAEQDKQVRFVRKAAESLLELVN
                     DLLDLAKIEAGKVDVRPVEFEVKTMFSALRGMLKPLLAGSSVNLHFEEAEDLPTLYTD
                     EGKVSQILRNFISNALKFTERGEIRVTASVDAERRNIAIAVADTGIGIHPADQARIFE
                     EFTQLDSPIQRKVKGTGLGLPLCKKLALLLGGQVTVDSQVGSGSVFTLTMPLYHEKAL
                     DNMLKPGGEAERELDPARLPVLIVEDHPETRLVYEKYLRNSRYQALPSSGLREARRLL
                     YQVRPAAIVLDLLLCGEDSWQWLAHLKSDERTREIPILIVTTVEDRAKAFMLGADAYQ
                     VKPIEAPVLIAELDRLTKKRDHVEPPAPDRPRIMIIDDEESFRYILRKCLSNIDCDVT
                     ESGDGMEGIRMCRELRPDLIFLDLNMPGRSGWQFLQDLAASEKLAGTPVVIVTSQSLS
                     NEARTLLEQSTRGIVMKSELSEEAIQHVVGELIPEAFARHHQS"
     gene            complement(15885..16919)
                     /locus_tag="PLS35_08110"
     CDS             complement(15885..16919)
                     /locus_tag="PLS35_08110"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein/SpoIIE family protein
                     phosphatase"
                     /protein_id="HNI18662.1"
                     /translation="MIKEQQMAGDQVQVPVGESTRVAEARRKAVELAGRIGLDETKTG
                     TCAVIASEMGTNLVKHATDGEMFVRSVTQGTMQGIELVAIDRGPGMSNVSHALQDGTS
                     TAGSSGNGLGAMMRMAAEFDIHSLPGRGTSIVARVWNGPVPVRQASVMKTGVIHLAKP
                     GETLSGDAWYSQCTGHRTLCAVVDGLGHGALAAQASQLAVETLRAHAQASLTEQVDLA
                     HRALRPTRGAALGIAEILHDQQLVKFVGIGNIMASICQVGSSRNMVSQNGILGHQIRR
                     VTEFQYPWPESAMLVMCSDGINTHWDINGYAGLAARDPSLIAATLYRDFSRGRDDTTV
                     VVLKKRTADG"
     gene            complement(16925..17329)
                     /locus_tag="PLS35_08115"
     CDS             complement(16925..17329)
                     /locus_tag="PLS35_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HNI18663.1"
                     /translation="MATLRDEIYPIRSSDDIVKVRQITREWALMQTFSLVDQTKIVTA
                     ASELARNTLEHGRGGEVRIQALQEGPRRGLRLTFTDQGPGIPDLAKALQDGYTTGNGM
                     GLGLSGSKRLMNDFDIQTEVGKGTTVMIARWK"
     gene            complement(17310..17699)
                     /locus_tag="PLS35_08120"
     CDS             complement(17310..17699)
                     /locus_tag="PLS35_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNI18664.1"
                     /translation="MERIPILKMGNFLLVTVQVDMHDRLAMTLQDDLTGRIVKDRAHG
                     VLIDISSLEIVDSFIGRMIGNIASMARVLDAETVVVGMRPAVAITLVELGLSLPGVRT
                     ALNVDQGVELLKESIEERAQGNGHVAG"
     gene            complement(17702..18565)
                     /locus_tag="PLS35_08125"
     CDS             complement(17702..18565)
                     /locus_tag="PLS35_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNI18665.1"
                     /translation="MEKRTKLAEIVRNQQDTILKDWVDFQLNAITVRRDLMKDSELRE
                     QSREFLNLFTQAAQTGSDVSEAIWKPVKDFLGQITRSRVLLGFTPSETATFIFSLKQP
                     LFDVIRAAVKDPKDLVSDMWTTTTLLDKLGLYVMELHQQNREEVIHRQQEELLELSTP
                     VVQLFENILALPLIGTLDSARTQVVMENLLQRIVETGATISIIDITGVPTVDTLVAQH
                     LLKTVAAARLMGADCIISGIRPQIAQTIVHLGVDLSEVTTKATLADAFMIALQRTGAT
                     VTKSAATRSKG"
     gene            complement(19033..19467)
                     /locus_tag="PLS35_08130"
     CDS             complement(19033..19467)
                     /locus_tag="PLS35_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI18666.1"
                     /translation="MRRIDLLTQACDPATLTVGQLMQDALFTCTPRTDALTIGRLMTK
                     QNFGGLPVIGEHGDLVGLVTEYDLLQAMIEGRDLRKVLAADIMTTHPLTAREDMRLEE
                     VANLFQDRYVTRLPVVRGALLVGIVARRDLLHGYMKASEYWS"
     gene            complement(19501..>19701)
                     /locus_tag="PLS35_08135"
     CDS             complement(19501..>19701)
                     /locus_tag="PLS35_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI18667.1"
                     /translation="RDLMSANPYSVRPETSLPTLAHVLTESDLLAVPVVNDKNRLLGM
                     VTRRDVVRAALRPAAKRRSAAG"
BASE COUNT         4038 a         6059 c         5546 g         4058 t
ORIGIN      
        1 cgcaatcgcc agcgccaggc attacaggcg acttcgatgc gttgcagcaa ctcctccgtg
       61 atgaaaccat agcgatgcgg ataactgtcg gtcagtccca tgatacattc gccgtggccg
      121 agcaattcgc gcaagcggcg gcgatagagt tgcggatctc tgcgcttcac gcggtgaatg
      181 gtcgccaaat agtcagccag cgcctgtgtc cgcttccggt ccaacggggt aggcctggtt
      241 gcgtccgcca gtcgttcgag atccttgtga tacgtgtccc cttccgacca ttcggtcagc
      301 aggaagaact ctttggccgc cgcgaccgac atcaactcgc cacgcgcggt gaacgcgcct
      361 acgtccagcg ccgtgatatg ccggggaagc cggctgtaac agtcatagtc catcaacatc
      421 gcctgggctc ggtcagccgg atgctcgtgg ccgaacggac cgggactcat cgtacccagc
      481 accacttggc gggttttgtt gccgtttgta cgaaaggtta atcggatcgg cgcgccatac
      541 ccgtactgtt tgtatcgtgc accctgggtt tcttttccga tggggccata ggccaccaat
      601 tcggccgtcg aaccgaaccg tgctttgacg tagccgtcca gggcggattt cttcaatacc
      661 ggcatatccg gctcgtacgc aaaacatgtt cccgaaaagg agtagccacg cccgccacat
      721 cgagccgaga ctggtgcatt gggcttcaac cccattcagg caagtgagga attatgcgcc
      781 agtggtatgc agacctccgc ccggcgtagt aagccttccc atgaaaatgc ctcactttga
      841 gccgattcac ccttgcctcg accgccatac aggctgattg tcaggggcat gtgccttgct
      901 gaggagagca gtgagagtcc ctgttccatc tgagacgaga ggaggacgca tgaacctgga
      961 agtggaaagc cggaatatcg aaatgactcc ccgctggaag acggaaatcg aagaacgcat
     1021 ggccgctttg caacgtggcc atgacgacat catccacggc cgcgttacgc tcaccaaaaa
     1081 tcgccatcac aagaaactgg acaatgtcgc cgaagcgctc gtgctcgtca cggtgccctc
     1141 gcggcagacc attacttcac gcaaagagga taagaccttc gaagaagcga tccggtccgc
     1201 cttcgatgcc gtggcgatcg agttgaaaaa atttcgcgag aaacgcgcgg acaaggtcat
     1261 tcgcgtggaa ccgttgccgc agcggcgcgg tgtcgtgagc aaggtctttc ctgaacaagg
     1321 gtacggcttc attctgatgg acggcggcgg cgaggtgtat tttcacaaga atgcggtcaa
     1381 gggcatggcc ttcgccgact tggaagacgg agtggaggtg ttgttcgaaa gcgagcccgg
     1441 cgagaagggc ttgcacgcca ccatcgttca gccaccgcat ctcctcaagc tttaggacgt
     1501 acacgatgct ggacaaccat agactcccgg cttctctcct ggctccgacg gtcgatccgg
     1561 cccgcttggg atttgccgat acgagccagg tgcagccgct cgaggagacg atcggacagg
     1621 agcgggcggt cgaggcattg gaattcggcc tgcagatgaa gagtcccggg tttaacatct
     1681 tcgtgtcggg ccctgtagga accggcaaag ggacgctagt ccgacagatg gttcaacgcc
     1741 tggctcagac cgctccgccc ccaccggatt ggtgttttgt ccataatttc caggacgcct
     1801 cgcgcccgtc ctgcctttcg ttttcagcag ggcaaggcgc gtccttcaaa cgcgatatga
     1861 gcaccttcat cgaaggtctc cgccgagaca ttcccacgac cttcgaaggg aaaaagtatc
     1921 tcgatgccaa ggcgaaaatc atcgaagaga cggaagataa aaagaagaat ctctttcatg
     1981 atctcacgat cctctgccac cagagaggat tcgtgtttga ggaaaccgcg ggggggttcg
     2041 cgctcgttcc catcaaagac gaccgcccaa tgactgggaa ggaaatggag gagctccctg
     2101 agtcaatcca ggaagaactg accgcgcggc ggcaatcgct ggagagcgac atccgggagt
     2161 ttcatgtccg gatgcacggc ctcgaacggg aagccgaaca ggcgttgcat cacttggacc
     2221 accagatcgt ggccaacgtc atgaaaggcg cctatgacgc tctcctgcaa acctatcaat
     2281 cctcgcagct cgtcgtcacg tatctgcagc gggtccatga cgatatcgtg caccagtaca
     2341 gagacttctt gcctcacact ggtcccatgc tggcaattcc aggcctggag cagtcacgac
     2401 gtcccgacct gacacgattc gccgtgaatt tgatcgtctc ccgggaggct gccggcggcg
     2461 cacccgtgat agacgaatca catccgacct acagcaatct catcggcaaa atcgagcgac
     2521 gtgcccatct tggcgtcatg tataccgact ttaccgaaat ccggcccggg tccatgttgc
     2581 aggctaatgg tgggtatctc atcctgcagg cgttggatct catgcggcag cccttctcct
     2641 gggaggcctt aaagcgcgtg attaagacgg gagccgtgac cattgaggat ccgtcggaat
     2701 tttttggatt cgccacggcc ggcctgcgtc cggagcccat tcccgtctcg gtgaaagtca
     2761 tccttgtcgg caccgccggc atctaccacc tcctgcaggc ctatgaggaa gatttttcaa
     2821 aattattcaa ggtcaaggcg gacttcgacg tggaagtgcc gcatgatgag caccaggaac
     2881 ggcagtatgc gcgattcgtt tcacgcttgt gccgggagga agggctgccc cacttcggcg
     2941 cggacgcggt cgccgaagtg atccgccagg gatttcggtt cgccgaccgc cacgaccgac
     3001 tctcgctgcg cttcagtctg gtgagcgact tgattcggga agccggctac tgggcccaaa
     3061 agagcgggca cgtcttggtg acccgcgcgg acgtcgaatc cgccctggcg catcaacggc
     3121 gccgggccga tttgcctgag cagtggctcc agggagaaat cgccgaaggc acattgatgg
     3181 tggatctcca aggcgaggtc atcgggcagg tcaatgggtt gtccgtctat gagctgggcg
     3241 attactgctt cggccggccg acgagaatca ccgcgcgcac ctacgtcggc acgaagggcg
     3301 tgattgatat tcagcgggag gtcgatctgg cgggtgagat ccacagcaaa ggcgtcatga
     3361 cgctggccgg tttcctggcc gggaaattcg ccggcatgca gccatttgcc ttgagcgcca
     3421 ccttgacctt cgaacaaacc tattctgaag tcgaaggaga cagcgccgcc gtggccgaac
     3481 tggcagccgt gctttcaagt ctggccgacc tgcctgtccg gcagtatctg gccgtgacag
     3541 gttccgtgaa ccagctggga gaaatccagc ccatcggcgg cgtgaacgaa aaaatcgaag
     3601 ggttttttga atcgtgcaag cggcggggcc tcacaggcaa gcagggtgtg atcattcccg
     3661 cccgtaacat caaacacctg gcgctgaatc gggaggtcgt cgcggcagtg gaggacggac
     3721 ggtttgccgt ctacggcgtc gatacggcag accaggcgct ggagttgctg atcggcgtgc
     3781 cggcagggga acgcggcgca gaaggcacct acccgccggt aagcatttat ggccgaaccg
     3841 ccgcacgtct cgaagagatg gcccagatcg ttgcaacctg gggtgaggcc gaagaggaca
     3901 acagggtcaa ggattagcaa aagcagtatc accttgcgag cgaggcgccc atgccgatgt
     3961 atgattacac ctgtttggat tgcgggaaag aatcgctgat cgtcgtcacg ctgaaacagc
     4021 acgaaaaagg cgaggtacaa tgcccggcgt gcggcagcac caaattgcag cagcattttt
     4081 cctcgttcat cgcacatacg acgaagaaga gttaaaggag acgcagcgat ccatgcggac
     4141 gttcacaacc ctcagcattc gtcacctgat tctcgccact gcagcaggcc tgatgctggc
     4201 gccagtggtc gacgacgcgc acgcccagga atttcctccg gatgccgcca aaggcaaggc
     4261 ggtgtacgag cggcattgtc tcgcatgtca cggcgccggc ggccgcggag atggccccga
     4321 tgctgccgga ttgacagtgc caccggccaa tttccaccgc ttcaaatcct tcctgaaatc
     4381 cgacgaagaa ctgcttcgga cgatcgagca cggcgttgtc ttcagtccaa tgcatgcgtg
     4441 gcaagggcaa ttgaccgaaa ccgagcggca ggatgctctc gcctacatcc gtctgctcgt
     4501 ccagcaggga aaatgacgca gcgccccgcc ggctgcgggc cgatgtcgta tggacgggac
     4561 ctgctacatg acgaacctca cctctcatga cgccttagtc gtggtggacg tgcaacggga
     4621 tttccttccg ggcggcgcat tgggcataca ggatggcgac aagatcgtcc cgattctggc
     4681 tgggtatatg gcacaattcc acgctcgagg cctgccgatt ttcatcactc gcgactggca
     4741 tccgcccgac cactgttcgt ttcgagacca aggcgggcct tggcccgtcc actgtatcgc
     4801 aggatcgccc ggctccttgc cgccaccgaa cttccagcct cccgtctcgg cggtcacaat
     4861 ctataaggcg atcgatcagg acaaagaagc ctattctaca ttgcagggca caccgcttga
     4921 tcgccattta cgcgccctga aggtccgccg cctgttcatc ggagggttgg cgaccgatta
     4981 ctgtgtgctg aacacggtga aggatgcgct ggggcttggt tacgatgtgt gcgtactcat
     5041 ggatgggatc aaggccgtca atctccaccc cgacgacggg cgcaacgctg aggaagccat
     5101 gttgcatctc ggcgccgtgc ccgtccgcct cgaaagcctg gccgcatgac accttcgacc
     5161 acctccctgc tcaccgatct ctatgaactc accatgacgc aggcctacct cgctcataac
     5221 atgacgggcg aagcggtctt cgaatttttt gtccgcaagt tgccgacccg caggaatttc
     5281 ttcatggcgg ccggactgga gcaggtcctc tcctttctgg agcagttcca tttctccgag
     5341 ggcgacctcg catggctcga ggagaggggc cgtttcccgc cagagaccct catcgccttc
     5401 cgagcgatgc gctttaccgg cgatgtccat gccatgccgg aaggcacggt gtgtttcccg
     5461 cacgaaccca tccttcgcat caccgcgccg ctgccgcaag cgcaacttgt cgaaaccagg
     5521 gtgatgaatc tcctgaattt ccaaaccatg gtcgcctcta aagcctgccg ctgcatcctt
     5581 gcagcctccg ggaagccgct gatcgatttc ggcctgcgcc ggtcccatgg tgctgaggcg
     5641 ggactattgg ccgcccgggc cagttatctg gccggatttg ccggcacgtc gaatgtcctg
     5701 gccggagcgc agttcgccat ccccacctac gggacgatgg cccattcttt tgtgcaagcc
     5761 caccgcgacg aaaccacggc gttcttacat attgcccagt cacaaccgca ccatgtggtc
     5821 ctgctcatcg acacctacga tacggaagcg gcggcagaac gcgtcgtcat gctcgcccct
     5881 gaactcaaac gccagggcat tgcgatccat ggggtacgtc tcgacagtgg agacttgggc
     5941 gctcatgcgc gcaaggtccg gcacatcctc gatcgcggag ggctggagga gaccacgatc
     6001 ctggccagtg gaaatctgga tgaagaacgg atcgaggcct tggtgacaag cggggcgccg
     6061 atcgatcgct ttgcagtcgg gacggccatg accacctcgg ccgacgcgcc ctacctggac
     6121 tgcgcctata aactccagga atatgccggg agaccatgcc ggaagcgttc agagggtaaa
     6181 gccacctggc ccggccggaa acaggtctat cgacggctgg gaccagatgg acgactagca
     6241 caggatacaa tcaccgtcgt aggggacact ctgccgggcc ggcctctcct gcggcaagtc
     6301 atggagggag ggcggcgact ggctccgagt ccctcgatcc tggaaattcg cgaccggaca
     6361 cgattggctt tgtcggaact tccggatacg ctaggccagt tggatcaggc tgagccttac
     6421 gaggttcgga tttccggcgc cctggaacgc ctggcgcagc aggtcgaccg atacacatga
     6481 ctccatgcga acaaccagcc gctccagcga ggagcggcgt ccacgcttgt gaaggaggcc
     6541 ctttgatgaa tcagcattcc ttgtccgcgg cattcgttgc cggattggtg gtgaccacgc
     6601 tctgttccat tccagacggc gcgctggccg cagcaaagtc cgcatcgaaa tccgcgctgg
     6661 ccgtcgcatg cgagagccag tatcagcgca cccgtcccgc gccaaaagtc gtggacaaag
     6721 tgttgcaggc ccatgcccgc tggctcgaag accgggaatc tgccgatggt cgccgggcga
     6781 atctctgtcg cacggacctg cgacaactgc ggctgacgac ggcttcactc gaacgggcga
     6841 atctcgaagg ggcaatcctg aagggcgcga atctgcggag cgccaacctg gttcaagccc
     6901 atctgaaagg ggcagacctg tctcacgcca ttctcgacga tgccaatctt gagggggcgg
     6961 acctgcgcaa aggcctgctg gccaaggctc atctcaatcg catctccgcg gacgaggcgg
     7021 ccttttacgg ggccaacctc cagggcgcat tctttcgtga agcgctgttg gagcgagcgc
     7081 attttgagga tgcagattta caactcgcag acctgagcgg agccagcctg ctcgatggat
     7141 atttctatgg cgcgaacctc tcaaaggcta atctaacgga cgcggaccta gccggaaccg
     7201 acctgcgccg aaccaatctc cggcaggcca ctctgcgacg ggccaacctc caaggcgcgc
     7261 tcctggacag cgccacactc gacggcacct cactggtcga ggcggatttg gaaagcgcct
     7321 atctggatga tgcctcgctg gtccaagcgg atcttcacga ggccagcctg agaggggcag
     7381 attttcgata cgcgcggctg accaacgcca accttcaacg ggcgaatctc gaaaacgcca
     7441 acatcgaagg cgccaatctg gccaaggctc gcttgaattc cgccacgatg accatgtcgg
     7501 tgttatacaa ggccaacctg acgtcagcga acctccatgg cgcccgattg catcatgctg
     7561 tccttatcga cgcgcacctg tcacgagccg acctgcagaa agcggatcta accgaagtct
     7621 atgcgcccaa agcccatttg cagcaagcgc ggctcgccga ggcgaatctt gaactggcca
     7681 atctggtatc cgctgacttg agcgaagccg acatgagtca ctcgatcatg gtccagacca
     7741 atttgcagga agccaatctg cgaggaacca acttgaccgc cgccgatttg actggagccc
     7801 aactcaataa cgccgacctt cagcaagcca acttccgcgg tgcgaacttg agcggcgcgc
     7861 ttggactcgt gcaggcacaa ctagaccagg cctgtctgga tgaggccacc caaatcccta
     7921 cagaccttca gcgaccgaag gcctgcccgc cgccacgtca gaagcgtaag tagggaactg
     7981 cccatcggaa tgtcggggca aacatccggg ctatcgggag gaacactcgg acaagggcta
     8041 ccatggcaag ggcggggcag agatggattg atgtagttga atgaagtgtt agtgcgattc
     8101 aggacaggtc acgcctccct tgccggtggg ctctcgggag atcaccgtga accttcggcg
     8161 caagacgatg ttcctgctgg ccgggctggc ggcactggcc gtgacgctcc tcctcatctg
     8221 gtgtccgcct gccctctgtc gggtcctgaa catttatacc tttcccctct ccaacctgag
     8281 cgactttcgg caatatgtgg cagatgaaga ggcgttctac tcactgcatc acacccgcgc
     8341 tgtccgtatt gattgtgcag ccctgcgcga tgaggtcgtg acggtcctca agatcggaca
     8401 tctggtgtta gaaaccaaca cacccggcga cactcaacgt gaacgtagac caggcacaat
     8461 cgaggcctgg catcaagggc ggctcttaca cctcacgaat acggcccttg cgtcgaagga
     8521 tgaacaacag gcctgggcca cctcggtgcg ggcagtcgtc acgctgcaac aacagcaaga
     8581 cctcgcattt ctttcccgac tgtttggcgc tgtcgtcctg catgtgtctc acaggcagat
     8641 acccatcgtg ctgttctccg atcgtccctg tctctagcaa ctccacattg tcgtcgtatt
     8701 ccccgcgccg cagactctgt actggtgacg gccagaacgc cgccgtcaca cctgaatcga
     8761 cataacgcgt tgagaacaca agtgctagtg cgctcttgct ccctatttcc tacttagggt
     8821 tgcgagccgg ctgaactagg cacatttcca gggtgccgga cacagaagca gcgcaaagat
     8881 gagagaggca cagatttacg cggcgaaggg aggatgcgat gaacatgaaa tggactcatt
     8941 gtctcccaat gttgttggcc ctctgcctcg cgttgccgac cgcctctcct gccggatctc
     9001 cacttccagc tgaaccggga gatgacttcc tcatcgaaca gacatttgat tcggtcgtcg
     9061 gcttattgtt tcgcgattac tcgctccaag ggaatggcca ggtagactat cgtacagcgc
     9121 gtcacatcct gggcatctcg tacgacgatc ccgcttcagc agaaccggat gtagccttgc
     9181 acccactctt ctactggtac gatcgcaatc aagacggcca atgggagtta tggatcgatc
     9241 gcgaagagga aggccatctg gtcgatgtca cacggtatga ctggaggcag ggcgacgatt
     9301 tactgacgct gtacccgaat ctgcggaatc ccacatgaca gccatacccg gcaattccta
     9361 taatctgtcc tgccgagcac ccagatcggc tcaaccgtca cggcacgacg ccgatagtta
     9421 tgcccatgcc ttcctctcac tcgcgtcaca agctgccttc gggtatctgg atcctgggct
     9481 tcgtcagtct gttgatggac atttcttcgg aaatgatcca cagcctgctg ccgttgttca
     9541 tggtcacaac actcggcgcg gggaccatcg cggtggggct ggtggaaggg ttggccgagt
     9601 ccctcgccct cgtggtgaag atcttctcag gcacattgag cgactatctg ggcaaacgga
     9661 agggattggc cctttgcggg tatacgattg gagcccttac caaaccactc ttcgcctggg
     9721 caccggacat cggcacgctc ctgacggctc gcttgctgga tcgactggga aagggcatcc
     9781 ggggcgcgcc tcgcgacgcg ctggtggccg atatcgcacc accgcagcta cggggagccg
     9841 cgttcggcct ccggcaatca ttggacacgg tgggcgcctt tctcggtcct ttgctcgcta
     9901 ccggattcat gctgctgtgg gccgacaatt tccgcgcagt cttctgggta gccgtcatac
     9961 caggcatgct ggccgtcgcg cttctcatgg tcggcataca agagcccgtc tcttcgtcat
    10021 cctcccgccg agctaatccg atcactcgtg cgaacttcag gcgtctcagt ccggccttct
    10081 ggtgggtggt gggaatcggc tccatcttta cgctggcgcg ctttagcgag gcgtttctgg
    10141 tactgcgcgc acagcagggc ggcatccctc tcgccttcat tcccctcgtg atggtcgcga
    10201 tgaatctggt gtatgcctcc tctgcctacc ccttcggcaa gctttcggac cggatcaatc
    10261 acaaaacgat gctcacatgg ggcctgctgg tgttgattgc cgccgatgtg gtgctggcga
    10321 ctaccaatca ttggggcgtg atattgggag gagtggcact gtggggcatt cacatgggaa
    10381 tgacgcaggg cctgcttgcc gcgatggtgg cggaccaggc gccgactgac ttgagaggta
    10441 ccgcgtatgg atgtttcaac ctggccagcg gactggccat gctcatggcg agcattgtgg
    10501 cgggattctt gtgggatcga ttgggggcgg cattcacctt ttatgcgggg gcattgtttt
    10561 ccagcatcgc tgtcatcgga atagtcgttt ctcccggcac tgctccagcc ccctctgccg
    10621 gccattccct tccaccacgc agtggcgagt gacgcgcgat tgcttcagac cacccacggc
    10681 cttcacgccg ttctggcagc tgcgcgaatg gcgacctgaa tcgtttcatg caactgttcg
    10741 atggcagcat ccttcggcag aaatgatacc gctcccgctg ctcgcatcgc ttcttcgatc
    10801 tgccagtcat tgtgtaccga cagccctatc accaacgtct caggctgctc gagcttgagc
    10861 cggcgtgtcg cctcgatgcc gtccatgccc ggcatattaa tatccatgat gacgattttc
    10921 ggttgcaggg actgtgcgag cagcagcgcc tgttctccat tattggcctc acccacgatc
    10981 tcgatatcag cgtacccttc cagaacggtt cgtaccccct gccgcagcat ggcatggtca
    11041 tccaccaaca gcacccgggt ccgggaggaa tgggcagact ccggcccggg cgacacagcc
    11101 agggcgtcga gcgccgacac gacttccggc ccagacagtg acgatgaagc catgtcggac
    11161 cgggagcgaa catccgtcga ggttgatcct cccacgtagg gaacgaccag cgtaatcttc
    11221 gtaccacggc cgggagcaga ttcgacggcc atgcgtcccc ccaacgcttc catacgttcg
    11281 cggatactga agaggccaaa catgggcgcg gcaatgttcg tcgtgctcgc caggtcaaag
    11341 ccgacaccct cgtccccgac gcacagatgg agcatatcgc tctcgatcca cacggagatc
    11401 gatgcctgcg aagtcttcgc gtgcttcgac acattaatca acagttcgcg ggccgactgg
    11461 aagatcaaca ccgcctgatc ctcgcgtagc ggcgcaagat cggcttcgca ttgcacggtg
    11521 acggtcattt catgccgacg catctgttcc gccagccatt tgatcgcgac caccaatccg
    11581 aactcgcgca gaaccggagg actcaattgc gccaccaaag acctggtgta cgtcaacgcc
    11641 tcgtccagca tatcgtctaa ttccttcacc aaggttgtcg ccggaccgag gttccctcgt
    11701 ttggcttgac taagcttcat acgaccaaag accagcagtt gcgccaggta gtcgtgcaat
    11761 tcagtcgcga gacgttgacg ctctcgctgc tcggtcaagg tgagatcgga tgcgagtgcc
    11821 cgcaggcgcg tgcgggacag cagcaattcc tccgtccgtt cggacacccg ctgttcaagt
    11881 tcgatcgtcc attgccgagt ttgctcttga accttctttc gttccgtgat gtcgcgtaac
    11941 aacagggtgt aggtgaccgc atcatccaga tggttttcac tgatggccaa ttccaccggg
    12001 aaactggacc gatccattcg aacgcccagc atctctcgtc tgagtccgct cacccctgag
    12061 tcatcgcgcg gttgatcgac gcccggagtg tctgcccctt caacggaata cagacttggc
    12121 aagagcaacg tgatatggcg tccgagcagc gcgtcagcgg gatatccgaa gagccgtccc
    12181 acagccggat tgacacgatg gatggtgccc tgatcgtcga tggtgatgat gccgtccaga
    12241 gcactgtcga cgatcgcctt gagccgcatt tcgctttcac ccagtgcgga ggagacgcta
    12301 tggcgctccc atgccagcgc caccaggtgg cagagcgttt ggaaaaatgc gatttcttct
    12361 tcggtaaatc gttctttccg gctggtcccg aaggacaatg ttcccaggat ctctccagtg
    12421 ctggctttca acggatggca ggcataggca cgcgcgccca tctcccgaac gaattttccg
    12481 cgcggatcgg ttgatatcaa ctcggcgtcg gcgacgagag acgttcccgc atcgacgaca
    12541 tatccgcaaa aggcttgccc agggtcgagc cgttgcgcct tcgccagcat cgcgtcaggc
    12601 actcccaatc cgaccagcag ctgcaggcct tttgttttct gatcgagcag aaagttcagg
    12661 cagagatcca catcgagatg cggcgcgacc ttttcgtaaa tggccctggt ccaggccgcc
    12721 cgatcgaccg gcatttccag gattaagcgg ctgatgtcaa gcaacagcgc ttgctgctct
    12781 ttcccgcgac gcagaatggc gtcttgttgc ttgcgatcgg taatgtcgag ggccgtgccc
    12841 aggatttgca acggttgccc attctcgtct ctcgagaaca cgacgttgcg gcagagaaac
    12901 cagcgccatt ctttcgatcg atgctggacc cgacactcaa attccaacac ctggccatcc
    12961 gcttggtccg gcaattgcgc cagctgcgcc atcaccagcg gcctgtcgtc cggatgaatc
    13021 catgactgca aggtttctcg accgacaaac tgcagttcat ctgacgcgta gcccagggta
    13081 gcaccggacc gggcattggt gtacaggttg cgctgttcac gcagatcgta gaggtagacc
    13141 gtggagggtg ccgcatcgac catgcttttg atgaaccgtt cccgctcggt gagttccttg
    13201 agcagaaccc ggctctcctg ctctcgatca tagagccgta ggagcgcctt ggccgcgccg
    13261 atcagctcgc tggcttcgac gggctcgctc agatagacgt ccgcaccgcc atcgagtccc
    13321 atcgcccgtt cctgcggtcg cacgcatgaa gcggaaacct gcaggaccat catatgttcg
    13381 gtcgcggggt tctttttgat caggcgacag atttcaatgc cgctgatatc cggcagccca
    13441 atgtcgagca gcaccaagtg cggctgttgc tcctgcacaa gccgtaaggt atccgcgccg
    13501 gtggccgctt cgaccacacg atagccggct cgctcaaaga ttttggtctt ggcgtagcgc
    13561 gccccttcgt catcgtcgac attgagaatg atgtgctgaa agatggtgct cacggtcctc
    13621 ttacaaccaa aagatcatga ctgatgatgg cgcgcgaagg cttcgggaat cagctcaccg
    13681 accacgtgct gaatggcctc ttccgataac tcgcttttca tgacaatgcc ccgcgtggac
    13741 tgctccagca gcgttctcgc ctcattcgag agggattgcg acgtcacaat cacgaccggt
    13801 gtgcccgcca acttttccga ggcggccaaa tcctgcagaa actgccagcc cgatcgtccc
    13861 ggcatattga gatccaggaa gatcaggtcg ggccgcagct cacgacacat ccggatgcct
    13921 tccatcccgt ccccggattc cgtgacgtcg cagtcgatat tcgaaaggca cttcctgagg
    13981 atatacctga acgactcctc gtcgtcgatg atcatgatac gcggacggtc cggagcaggc
    14041 ggttccacgt gatcccgctt cttcgtcaga cgatccaact cggcaatcaa caccggtgcc
    14101 tcaatcggtt ttacttgata ggcatcggcg cccagcatga aggccttggc ccgatcctcg
    14161 actgtcgtga caatgagaat ggggatctcg cgcgtgcgct catccgattt caaatgcgcc
    14221 agccactgcc aactatcctc gccgcataac aaaagatcca gcacgatcgc cgccggccgc
    14281 acttgataca ggagccggcg ggcttcacgc aggccgctac tgggcagcgc ctgataccgg
    14341 ctgttccgca gatatttttc atagaccaac cgtgtctccg gatggtcctc gacaatgagg
    14401 acgggaagtc gagcagggtc cagctccctc tcggcttcgc cgcccggctt cagcatgttg
    14461 tccaacgcct tctcgtgata gagcggcatg gtcaacgtga ataccgagcc gctgccgacc
    14521 tgactatcga ccgtgacctg cccgccgagc aagagggcca gttttttaca cagcggcagg
    14581 cccaaccctg tgcctttcac ctttcgctga atcgggctat ccaactgggt gaattcttcg
    14641 aaaattcgag cttgatcagc cgggtgaatg ccgatgccgg tatcagccac cgcgatggca
    14701 atattgcggc gctccgcatc gacggacgcc gtgacgcgaa tctctccccg ctccgtaaac
    14761 ttgagcgcgt tcgaaataaa gttccgcagg atctgcgaga ccttcccttc gtccgtgtac
    14821 aacgtcggga gatcctccgc ttcttcgaag tgaagattca ccgacgagcc tgccaacagg
    14881 ggcttcagca tgcccctgag agcgctgaac atcgttttga cttcaaactc gaccggtcgc
    14941 acatcgacct tgccggcttc gatttttgcg aggtccaaga gatcgttgac cagctccaga
    15001 aggctttccg cagccttgcg cacaaatcgc acctgcttgt cctgttcggc cgtcagttct
    15061 ccatcggacc ggtccaacag cagtcccgac aaggccagga tcgaactcaa gggcgttcgg
    15121 aactcatggc tcatgttgga caagaaccgc gtcttcatac tgtccgcccg acggaggtgg
    15181 tccgcctttt catccaactc ggcgtagaga gcgacgacgc cacgattggt atcctccagt
    15241 tcctgattca tacgaaccag ttcatcctga cggagacgca attcatcaag ggcccgcaag
    15301 agttcatggt tttgctgccg taactcctcc tgcgcattct gggcgggcgt ttggatcaat
    15361 tgatccacca ggctcgagag ttctttcgcg cccaatgctc tggtcttcga cggaaagacc
    15421 tttttcagca gcaccctcgt gcctttcccc acggcactgt cgatttcgca ttggtccatc
    15481 aaacgtttgg caccaacgat acccagcccc attccggtat cagactgata ccgtccctcc
    15541 aaaatgagtt tcagatcggc gatgccgggc ccgctatccg tcacacgaat caacaagacc
    15601 tgcggagcgg tctgcccttc gatcagaaac tccaccgccc cattcctggc ataacgaaag
    15661 gcattccgcg cgatttcgga caccgccgtt gcaatgcgtg tctgatcttg cgcatcaaag
    15721 ccgatcaacc cggcaatctg cctggcccgc tgacgcgcag ccacgacatc atcttcgtac
    15781 ttcagcgcga cattgatgat cgcataactc atcgcgtgtg acctttcgag gcggaaccca
    15841 tcctgtcatt ccttcagatt cgcgcgggac actccaggcg catctcaccc atccgccgtg
    15901 cgcttcttga gcacgactac cgtcgtatcg tcgcggccgc gggaaaaatc gcggtagagc
    15961 gtggcggcga tcagactcgg atcgcgtgca gccagcccgg catagccgtt gatatcccaa
    16021 tgcgtgttaa tgccgtccga acacatcacc agcatcgccg attcgggcca gggatactga
    16081 aactccgtca cgcgtctgat ctggtgcccc agaatgccat tctgcgagac catgtttctg
    16141 gagcttccca cctggcatat ggacgccatg atatttccga taccgacaaa tttgacaagc
    16201 tgttgatcat gaagaatttc tgcaatgcca agggctgcgc cccttgtcgg ccgaagcgcg
    16261 cggtgcgcaa gatctacttg ctcggtcaga gaggcctgcg catgggcgcg cagggtttcc
    16321 actgccagct gtgacgcctg cgccgccagg gctccgtggc ccaacccatc cacgaccgcg
    16381 cagagcgtgc gatgtcccgt gcactgggaa taccaggcat caccggaaag agtctcgccc
    16441 ggcttggcta ggtgaatgac gcccgtcttc atgactgagg cctggcgaac tggcaccggt
    16501 ccattccaga cgcgggccac gatgctcgtc ccccgcccgg gcagggaatg aatgtcgaat
    16561 tcggctgcca tccgcatcat ggcgcctaaa ccgttcccgc tgcttccggc cgtagacgtc
    16621 ccgtcttgta atgcgtgact cacattcgac atcccggggc ctcggtcgat cgccacgagc
    16681 tcaatgccct gcatcgtccc ctgcgtcacg ctccgcacaa acatttcccc gtctgtcgca
    16741 tgcttgacga ggttcgtgcc catctcagac gcgatcaccg cgcaggtccc tgtcttcgtt
    16801 tcgtccaacc cgatccgtcc ggccaattcg accgccttgc ggcgcgcctc ggcgacccgg
    16861 gtggattcgc ccaccggcac ctgcacctga tcgcccgcca tctgttgttc cttgatcatc
    16921 gctctcactt ccaacgcgca atcatgactg ttgtcccctt accgacctca gtttggatat
    16981 cgaagtcgtt catgaggcgc ttcgagccac tcaggcccaa tcccatgccg tttcccgtcg
    17041 tgtatccatc ctgcaaggcc ttggcaagat cgggaattcc cggcccctgg tcggtaaacg
    17101 tgagtcggag cccccggcgg gggccttcct gcagcgcttg aatacgcacc tcgccgccac
    17161 ggccatgctc gagcgtattg cgcgcgagct cactggcggc cgtcacgatc ttcgtctgat
    17221 cgacgaggga gaaggtctgc atcagcgccc attccctcgt gatttgacgg accttcacga
    17281 tgtcgtccga agagcggatg ggatagatct catcccgcaa cgtggccatt cccttgagct
    17341 ctctcttcaa tggactcttt cagcagttcc acgccctgat cgacattgag cgcggtgcgc
    17401 acaccgggga gcgacagccc taactcgacc agcgtaatgg cgacagccgg acgcatgccg
    17461 acgactaccg tctccgcgtc caagacccgc gccatgctgg cgatgttgcc gatcatgcga
    17521 ccaataaacg aatccacgat ctcaagcgac gaaatgtcga tgaggactcc gtgagcgcgg
    17581 tctttgacga ttcgaccggt caaatcatcc tgtaacgtca tggcgaggcg atcgtgcatg
    17641 tccacctgga cggtgaccag gaggaaattc cccattttta aaatcgggat cctctccatg
    17701 gttatccttt cgacctggtc gcagcagact tcgtcacagt agccccggtg cgctgcaagg
    17761 caatcataaa ggcatcggcg agcgtggctt tggtcgtcac ttcgctgagg tcgacaccca
    17821 gatgcacgat cgtttgggca atttgcggac gaatgccact gatgatgcaa tccgcaccca
    17881 tgagcctggc cgccgcgacg gttttcaaca gatgttgcgc cactaatgta tcgacggtcg
    17941 ggacgccggt aatgtcgatg atggagatcg tcgcgccggt ttcaacgatc cgttgcaaca
    18001 ggttctccat gacgacctgg gtccgtgcgc tgtccaaggt accgatcagc ggcagggcga
    18061 ggatgttctc aaacaactgg acgacgggcg tcgacaactc caacagctcc tcctgctggc
    18121 gatgaatgac ctcttcccgg ttctgttgat gcagttccat cacgtataac ccgagcttgt
    18181 cgaggagggt cgtcgtggtc cacatatctg agacgagatc tttcgggtcc ttgacggcgg
    18241 cacggatcac atcgaacagc ggttgcttca gagaaaaaat aaatgtggcg gtctcggagg
    18301 gagtaaagcc cagcagaacg cgagacctgg taatctgccc caggaagtct ttgacgggct
    18361 tccagatagc ttcactgaca tccgatccgg tctgcgccgc ctgggtgaag aggttcaaga
    18421 attcccgcga ctgttcccgc aactcactgt ccttcatgag atcacgtcgc acggtgatgg
    18481 cgttcaattg aaaatccacc cagtctttga gaatggtgtc ttgttgattc ctgactattt
    18541 ccgccagttt cgttcgcttc tccatgccct tccagcctcc tctcacatca aattgtaccc
    18601 atgcaattac ggcgggactt catctggaat gacaaacggg ggcgggtaac tgtatgcgcc
    18661 ccgctggtct atctcccact aggaaaaccc ctggcaattc ggcacagcct atcgaaaatc
    18721 gttttggact tccagaggga acaacaaggg ggtttttcgc cgcccgggag cggctgcggg
    18781 ggcctatgag caggagaaag tgagccgaat caacggtggg atgtcgatgc cgcgccttgg
    18841 ataactaccc atggaccgtt ggggaactcc tgcagactgt tcgttcattc atgcctgcta
    18901 tggcccaggc gcaacgcgct tttgaatctt ggtgaggtac ggtgttcgaa ggtgtagagc
    18961 aggagaatgg atacggtcgg cggcacttcg cccctccaga tgggacagga aaagagaatc
    19021 tatcccatcc ggttacgacc agtattccga cgccttcata tatccatgta acaggtcccg
    19081 tctcgcgacg ataccaacaa gcagcgctcc ccgcacaacc ggcagacgcg tcacgtagcg
    19141 atcttgaaag agattagcga cttcttccaa tcgcatgtcc tcgcgagcgg tcaatggatg
    19201 ggtggtcatg atgtcggctg ccaagacctt gcgcaaatcc cggccttcga tcatcgcctg
    19261 cagcagatcg tattccgtca cgagaccgac caggtcgccg tgttccccga tcacgggcaa
    19321 tccaccgaaa ttctgcttcg tcatgaggcg gccgatggtg agcgcatccg tacgaggcgt
    19381 gcaggtaaac aaggcatcct gcatcagttg tcccaccgtc agcgtggcgg gatcacaagc
    19441 ctgcgtcagc agatcgattc ggcgcataca tgctccttta cgatcggaca gcggatccgg
    19501 ctaccccgct gcagatcgtc gtttcgcggc gggccgcaat gccgctcgta ccacatcccg
    19561 gcgcgtgacc atgcccagaa gccggttctt gtcatttacc accggcaccg caaggaggtc
    19621 gctttccgtc agcacatggg ccagcgtggg caaactggtc tcaggccgca ccgaataggg
    19681 attcgcgctc atcagatccc g
//
LOCUS       DAOQEW010000056        19447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_712_length_19447_cov_8.208333, whole genome shotgun sequence.
ACCESSION   DAOQEW010000056 DAOQEW010000000
VERSION     DAOQEW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19447
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_712_length_19447_cov_8.208333"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..2449)
                     /locus_tag="PLS35_08140"
     CDS             complement(404..2449)
                     /locus_tag="PLS35_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNI18668.1"
                     /translation="MTGALRYVVLWLALILWAAPLHAHDPDAVDLELPEVEVSADRPV
                     AASSQQFIPDKEYLMQPQGRPAQVLRLIPGFIAVEHSGGAGKADQYFLRGFDADHGTD
                     VAFFTDGMPINLRSHAHGQGYTDLNFIIPETIEGVDVYKGAYLPEYGDFATAGAVNFR
                     TRDMVKEGVVQSAGGQFNTQRHLLMFSPTKDTVRTLFAAEGYYTDGPFQNDNRYFRGN
                     LLGKATMNPLGRDELVITGTFQKSQWNGSGEIPLRAVHDGSLDRFGSIDPSEGGKTLR
                     STGRMNYHYDMPSGGRFFANAYAQYYRFDLFTNFTFFLNDPVNGDGFQQSDRRVVYGG
                     DVGYRHSGHLFDMDGAATVGVQARIDDIHARLAPQVKRAPLGSTADSTIFEASYAPFL
                     KLELQPTPWLRLAGGVRTEVFTFDVRNRCANCAEQPFGNTSTGLVLPKMNVILGPWFE
                     TEFFANYGEGYHSNDARSIVARAASPLAKARNYEVGVRSKPWGPDGVELIGTLWALDL
                     KQELVFVGDAGTTEIRGASRRRGLEVAARGQVWGPLYFNGSVTWTKAEFTNGDAIPLA
                     PEVTAYGALLLRWPEGLTSQLQATYLGVRPLVEDRSAKAPSWTTFDLSERYQLPVKLP
                     HGRLEAFLFVQNLFNTKWEQATFFFDSRLRNEAAGVADTHFVPGSPRFVMAGLAWYF"
     gene            complement(2457..2873)
                     /gene="nikR"
                     /locus_tag="PLS35_08145"
     CDS             complement(2457..2873)
                     /gene="nikR"
                     /locus_tag="PLS35_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNI18669.1"
                     /translation="MDALARFGVSLDRHLLVDFDRLIERRKYTNRSEAIRDLIRDSLV
                     GQEWDENKDTVATITFVYDHHVPDLARKLTHIQHEFEGHIMAGMHVHLDHDHCLEVLV
                     ARGKGSAIRNVADALLSVKGVKHGKLTMTTTGKGLR"
     gene            complement(2933..3808)
                     /locus_tag="PLS35_08150"
     CDS             complement(2933..3808)
                     /locus_tag="PLS35_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNI18670.1"
                     /translation="MRVRRWNARSVWYLLAGLLVALAGWGFWWEPASLSTREYDLVLP
                     GWPAAAGDLRIAVLADLHTGSPHNDLEKLQEVVASTNGTHPDLILLAGDFVIQGVVGG
                     RFVEPEASARVLRSLQATLGVYAVLGNHDWWLDGPRVRKALEAEGIPVLEDRAIPLVF
                     NRMPFWLAGVGDFWEAAHDVRRALTGVDEASPVIVVTHNPDVFPEIPARVTLTIAGHT
                     HGGQVAVPGLGRPVVPSQFGERYAIGHIVEQGRHLFVSTGIGTSILPVRFRVPPEISI
                     VTLRSAAPLSQPPRD"
     gene            complement(3990..5063)
                     /locus_tag="PLS35_08155"
     CDS             complement(3990..5063)
                     /locus_tag="PLS35_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18671.1"
                     /translation="MQDVDLSTIKLLLVDDEAHCTKLMEAFLRQAGFSRITMTNDSRQ
                     ALELFQQVEPDLVALDMRMPHMDGFDVMRQLRQVIPADDYVPILIVTGELDAPTKHKA
                     LTEGANDFINKPVDATEVVLRIKNQLQTRRLHGQLRMHNEHLEAQVRLRTKVVEQTQL
                     DLLNRLVMASEYRYDPKGAHAWRVGRLAMLLSEMKGLPLEQTDMIKKTAPLHDVGKIG
                     LLDSIVMKEGSYTAIEWEAMKTHTKIGSKLLADSRSPLLMMAREIALTHHERWDGTGY
                     HRLKETQTPLPGRIVALVDAFDVMTHPRSYKGQLNLVEARAEIERHAGTQFDPELSAL
                     FLKLIDREGESLLADSTAVQLFA"
     gene            complement(5117..7870)
                     /locus_tag="PLS35_08160"
     CDS             complement(5117..7870)
                     /locus_tag="PLS35_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18672.1"
                     /translation="MQIEYSFLRTRLGRRVFVLFILCALLPVGVLSVVTIPQVTKYLR
                     TQAEEHMRQQSRAMGMAVYQRLLLLEEELLAITARQQVGASGRSEEWPSRPLSSRSGF
                     IGMASVSEADGTAVFGGGQRSRAFSSAERAILRQNKTLLTVEPAGVDRPRFFLSTMSE
                     QDGRVVVAEIKHDFLWDVGDSLAPPPDTSLCVFAQAGQTLLCTDAASVALSEQWLAQG
                     KASSGSFTWRDGDDEKLARAWGIPLRSRFSTDSWVVVLSKPMSVVLDQASDLRRQVRL
                     AIIAAVFASALLGVVQIRKFMGPLEQLSEGTRRIAARNFSTPVQVRSGDEFELLASSF
                     NAMAGRLNQQFTQLATIHEIDRSVLSVLDPSRIVDTVLTRVREVSPCDGIAMLLMDSS
                     DPARGWLYVRSGAKNKPTTMTGVSVGEEERRMLASHEGGTALIGVDGAAAFATLCRPG
                     VTALMVLPLFRSRDLLGAIAMSYRQAPEMDSEHKTQLRQLADQVAVALSNASDLAERK
                     RAEASLRESHTRLEQAMTDLQTAQQQMVQQERLRALGQMASGIAHDFNNTLSPIMGFS
                     ELLLIAPQMSADPAQLKEYLQTINMAAKDAAKVVSRLREFYRPRLDADLAGIVDLNRL
                     VEQSVKLSQPKWKDQALANGVAIEIKTELASVPPVAGHESELREVLTNLVFNAVDAMP
                     KSGSITLRTKADGDHVRLEVSDTGTGMTEEVRQRCLEPFFSTKGEKGSGLGLAMVYGI
                     IKRLRGTIEITSAVGVGTTFSIRLPIQSEQEAGTKQEGNSLAGVRLQILVVDDDPLVG
                     RVTGEYLRVAGHQVEVVTSAAEAVKRFNPERVHLVVTDHGMPNMTGRQLAEVIKQVSP
                     DTPVVLLTGGDEIEVTETASQAVDAELSKPLTMAALQRTLSTLTFPGSLPAESRKEIG
                     DAA"
     gene            complement(7886..8650)
                     /locus_tag="PLS35_08165"
     CDS             complement(7886..8650)
                     /locus_tag="PLS35_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI18673.1"
                     /translation="MPHIPFDRRQQGFTIIELAIVMVIVGALAGLAVPMYVGYLDKAR
                     LARCIAEIRYISRAVDSYNAAYDVYPNTLAEAGAGEIVDPWGNPYEYLNIAALSLPGN
                     GGGNGNAGGNGGGGGSGGNGSSGGGNGNTPGGSGGGGTGSSSGGTGGTNATTPPSNNG
                     NGGGGGNHGAWDWLLPVEAYAAGNANGNGNGNGSGNASKGKPRKDRFLHPINSDYDLY
                     SMGKDGESVEPLTAKKSHDDVIRANDGSFVGLAVEF"
     gene            complement(8862..9497)
                     /locus_tag="PLS35_08170"
     CDS             complement(8862..9497)
                     /locus_tag="PLS35_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNI18674.1"
                     /translation="MSRPRFRRHFLWDTVQPRFVGLSLLYVVVVIAAVSGALFLPLML
                     QLDHLPLSSVEAQRVADQFELLHSRFWPAVGVVSLLLIVHGVFFTHRIAGPLYRFRRI
                     FQSVANGDLTVRTSIRKSDYLHAEAQCLGEMVSALREKIGRIEAHHADMAPQLERLKA
                     AAARGALREVEQEADRLRATVEQLAQAMEPFQTTSEPVEPRPTPLPTAAGF"
     gene            complement(9494..9868)
                     /locus_tag="PLS35_08175"
     CDS             complement(9494..9868)
                     /locus_tag="PLS35_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18675.1"
                     /translation="MWSIRAVSCLLVLWWCAACAPEPDQAPSYLIGVWETTTDGYVDQ
                     HMFIDQTRIGFGTSALAADGYMITKVDEGRDGARTLFVVSYQDAEQAKYQLSFYYDPA
                     GGGRITFKNQDHLVWTKRAATT"
     gene            complement(10045..11478)
                     /locus_tag="PLS35_08180"
     CDS             complement(10045..11478)
                     /locus_tag="PLS35_08180"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNI18676.1"
                     /translation="MSVRRSQPGLVALGIVGALLGCWPEGPASAADMLAPRFSKVSTN
                     GDLQAQVRATAAKVLPAVVSIASTVMVHDQGFSDEGLPFGMFKDVPPRRQYGQGSGVI
                     VSSDGYIITNNHVVADAVDVEVILADRRQFKGRVVATDPKTDVAVVKINASGLPTAAW
                     GDSSALAVGDFVLAIGNPLGLSRTVTFGIVSAVGRADVGVADVEDFIQTDAPINPGNS
                     GGALVNTNGELVGINTAIASPTGGSVGVGFAIPSNMARTAMQSLIKTGRVVRGFLGAS
                     TQDVTPLLAKIFHLPDVKGSIITDLQAKGSAERAGLKRGDVVVRFDGRDVMDSGHLRN
                     LMAAAAIGSKHRVELLRDARLMQAELTVQEAPRERQRKTQSAETAGPTAHPLSGVIVD
                     EITPALARQMDLPSNTGLVVTDIEDGSLAEVSGLQPGDLLLELNRQPIPNFSTFQRLA
                     EPLRSTDLALLLINRQGSLLYIPIQTE"
     gene            complement(11546..14443)
                     /locus_tag="PLS35_08185"
     CDS             complement(11546..14443)
                     /locus_tag="PLS35_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNI18677.1"
                     /translation="MARDPVCGMTVEPATAAGYAEYEGETYHFCSPSCRDRFRAAPAH
                     YVKKAAPAGAAVQPSSRRSLPMMQAMPAQEATTGGERDPVCGMMVEPATAAGSHTHEQ
                     KTYYFCCQGCLEKFRADPGRYLAPVAAPASTSLRQFGGKALPMLGAASSPGPSAAAVI
                     DPVCGMTVDPATAAGSFAYQDTTYFFCCQGCLTKFRADPARYLSPGSVKDAMPATPVP
                     PGTKYVCPMCPEVLEEKPVPCPKCGMALEPDSVQPLPTKTEYVCPMHPEVVQAEPGAC
                     PKCGMALEPRTVTVAEELNPELVDMARRFWVALGPAAIVFLLAMSHMLPGHPIQHLLS
                     DAQSAWVQFVLSTPVVLWAGWPFFQRGWASIVHRSPNMFTLIAIGTGTAYLYSVIATL
                     FPFSIPQSFHLESGAVPVYFEAAAVITVLVLLGQVLELRARSRTTGAIRALLGLAPKT
                     ARRLFDDGREEDVPLEQVQVGQRLRVRPGERVPVDGVILEGATSIDESMITGESIPVE
                     KFAGERVTGGTINGSGGVVMRADRVGADTLLSHIVQMVAEAQRSRAPIQRTADVVAGY
                     FVPVVVSVALVTGILWAWLGPEPRLAHALLNAVAVLIIACPCALGLATPMSIMVGTGR
                     GASAGVLFKKAESLETIEKVTTLVFDKTGTLTEGKPKLRVVSAMPPWSETDLLRLAAS
                     VERGSEHPVANAIVGGAEARGLNLEPAAGFTSKAGKGVMATVGTKRVAVGTIDWLRDL
                     KVGDEAALAALDANAELMRQTGQTVMLVAVDGQAIGLLGVADPIKSSTPEALRLLRQE
                     GIRLVMVTGDHAVTAQAVAKELGLDEVRAGVKPEDKSRIVQELQRQGQVVAMAGDGIN
                     DAPALAQADVGIAMGTGTDVAMENAGVTLVKGDLRGIVRAYRLSKATMRNIRQNLFFA
                     FVYNSIGVPIAAGLFYPVFGLLLSPMIASAAMTGSSLSVISNALRLRQTDL"
     gene            complement(14552..15196)
                     /locus_tag="PLS35_08190"
     CDS             complement(14552..15196)
                     /locus_tag="PLS35_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNI18678.1"
                     /translation="MVMRRVWHSGLFVGVMVMALVGLAGTAQAAKPELKGNFQVLSEE
                     KSTHKPGKVQLVEFADFYCPHCHRFDGEGLMILEKEFGNKVEAVMVGYPVIPGKLPTA
                     FDMYEQAKSMGKGNEMKRALFRTIHKDKVGIIDKAIREVLIREVGLNPAAFEEGLASA
                     KPAKAFEDGRKWGDRIKIQQTPTVLLDGNIKVEQIDPENLKLIIQSILDGDSKK"
     gene            15293..15844
                     /locus_tag="PLS35_08195"
     CDS             15293..15844
                     /locus_tag="PLS35_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNI18679.1"
                     /translation="MRGKLLQGLGILCMTAILAACATSADQREAEDGASQTPQFSQIK
                     AAPESFKGQTVVLGGQVLSARRMKDGTRLEVLQLPLNDAQQPSLDLTKSQGRFIAIRR
                     EFLDPATVPQGTFVTITGQMTGSVTQPLDETDYAYPVLEITTFRTWIPSQDLNLPRYR
                     PYPYYSPFWHPYWGPYGRFPYYW"
     gene            complement(16000..16479)
                     /locus_tag="PLS35_08200"
     CDS             complement(16000..16479)
                     /locus_tag="PLS35_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18680.1"
                     /translation="MLSRSAVVLFGALSLLSSATGAWALQAGGVEVGDLETGSVVGQP
                     FKELEVDAQLFAVEVGGLKAWYPPTTIIDFKVRPGRPVLLKVTNNTSSDRGFQMTDPV
                     NAASPFVLKAEVVLKPGETKYIGVPTSDLFNATQGNTLTYRDQLNPKQPGGQLIMIK"
     gene            complement(16498..16842)
                     /locus_tag="PLS35_08205"
     CDS             complement(16498..16842)
                     /locus_tag="PLS35_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18681.1"
                     /translation="MRCVCIVLIQLLVVAGGTAGWGADDSLEHLLRAQAFNKEFEGFD
                     FYYVNIEADHPQPDGSHEVLAIASGKFSDSTKRMKVLFLIVDGLIVGGQVLEGTGLPP
                     CLAPEHRSSSSL"
     gene            complement(17084..17386)
                     /gene="smbP"
                     /locus_tag="PLS35_08210"
     CDS             complement(17084..17386)
                     /gene="smbP"
                     /locus_tag="PLS35_08210"
                     /inference="COORDINATES: protein motif:HMM:NF028095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNI18682.1"
                     /translation="MTGLRAGVVVASLCALVAVAPANMALADGGGHVKETIKHAKEGV
                     EHEKEAIKHLEEAVKGSNDPHAKEALEHAKEAVKHAEESLAHAEQASGKKGKGKGN"
     gene            complement(17596..18120)
                     /locus_tag="PLS35_08215"
     CDS             complement(17596..18120)
                     /locus_tag="PLS35_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18683.1"
                     /translation="MPVSDAVASPQRTTVAGKPYDQTGLNVAVVRLLALALDISGGWS
                     PAPPAAGLMIPFGYRDWPHLTSLVQSSGGAQELRFYVCPKGLLTADDRSFPVGTVFVV
                     ESEPCPVGIGAQESRPAVFVMEKCAGMTRDEVGTAQSELWIYASWDSGGQSARADPGR
                     CGICRLPWLTPASA"
     gene            complement(18152..>19447)
                     /locus_tag="PLS35_08220"
     CDS             complement(18152..>19447)
                     /locus_tag="PLS35_08220"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18684.1"
                     /translation="LPPRGEWDGLPLHPGPSGAGKVKTGARNGNDGMSPESIEGVEDS
                     TIMSDRMNRRWYRMGFVSLVALIVLCAVTGSGATEPDGWMQRFEAGVKAREAARYAEA
                     EPILMQVLREAEQGGADDARVALAANQLGLLYHDQGRLSEAEALYLRALGTWEAQLGP
                     EHEDVAAALNNLAEIAHAKGEWAAAEPLYERVLKIERQTLGPAHPDVAISLNNLAELY
                     RKQGRETAAESTYRLALTLMEQAHGPDHVELGPVLNNLAALYKGRGMYAWAEPLYERA
                     LKVRRLRFGNDHPAVVMSLNNLACLYQAEGLYASAQRHFDEALPLAERLSGPQSALAG
                     TIIGNMAFLADQQGLMAQAQLWYQRALVIQQQQLGVHHPTVGLLVERYAVVLNSIGQS
                     VEAGLYAARAASIRARSQPGAVKQGAVSYLGATVGEASR"
BASE COUNT         3976 a         6354 c         5321 g         3796 t
ORIGIN      
        1 gaaatactca cgaggaagaa tcatggccgc tcctaaaaaa catccggaca agcccgaatc
       61 gtaaatgcct atgcccttta ctttcaatcg ctgacatcac cggaacttct ttgttcagtt
      121 cccctcgcga ccgctcaaac cgtcatccag caaagcctca gctgacgcag cagcggagtc
      181 ccgatccttg agaccgctca aaaatggctt cccaacaagg ccgcaggtga gaaaataccg
      241 caggcgtacc cgcattctgc tggtgatgtg ccctcagaca ggccgctcaa aatgtccgtt
      301 cagctaggcc gcagccgagg gtagcgccgg aggcgtagcc cgtggctacg ttgaggacgg
      361 taccgaggcg agaacaacgc tggcgggcat tttcagcagc ctgtcagaag taccaggcga
      421 gaccagccat cacaaaccgt ggactccccg gcacaaagtg cgtgtccgca acgccggccg
      481 cttcgttacg tagacgggag tcaaaaaaga acgtggcctg ctcccatttc gtgttgaaga
      541 gattctgcac aaagagaaat gcctccaacc ggccgtgcgg cagtttcacc gggagttgat
      601 agcgctccga caaatcgaac gtagtccagg acggcgcctt cgcactgcgg tcttcaacca
      661 gcggccgaac ccccagatac gtcgcttgca gctgggatgt cagcccttcc ggccaccgca
      721 acagcaacgc tccataggcg gtcacttccg gcgccaatgg aatcgcatct ccgttcgtga
      781 attccgcctt ggtccaggtc acgctaccgt taaagtagag cggcccccag acctgacccc
      841 gtgccgcgac ttccaggccg cgccgtcgcg aagccccacg gatctccgtc gtccctgcat
      901 ctcccacaaa gacgagttcc tgtttcaggt ccagcgccca caaggttccg atcaattcaa
      961 ctccgtcagg cccccacggc ttcgaacgaa caccgacttc gtaatttctc gctttcgcca
     1021 atggtgaggc agcccgggcc acgattgagc gggcgtcgtt actgtgatac ccctcgccgt
     1081 agttcgcaaa gaattccgtc tcgaaccagg gccccagaat cacgttcatc ttcggcagca
     1141 cgagccccgt actcgtattg ccaaacggct gttccgcaca gtttgcacaa cggtttcgca
     1201 cgtcgaacgt gaagacttcg gtgcggacac ctccggccag tcgcaaccaa ggcgtaggct
     1261 gcagctccaa ttttaagaag ggcgcgtagg aggcttcgaa aatcgtgctg tccgcggtgg
     1321 atcccagcgg cgcccgtttc acttgcggtg caaggcgcgc atggatgtcg tctatccgtg
     1381 cctgtacacc cacggtcgcg gctccgtcca tgtcgaacag atgtccactg tgacgatacc
     1441 ccacatcacc gccgtagacc acgcgccggt ctgattgctg aaagccgtcg ccgttgaccg
     1501 ggtcgttgag aaaaaacgtg aaattcgtaa acagatcgaa ccgataatac tgcgcatagg
     1561 cgttggcaaa gaaccgcccg cctgatggca tgtcgtaatg gtaattcatg cggcctgtgc
     1621 tacggagcgt cttcccgcct tcgctgggat cgatcgatcc aaagcgatcc aacgagccat
     1681 catgcaccgc gcgaagcgga atctcgccgg acccgttcca ctgcgacttt tgaaacgtgc
     1741 cggttatgac caattcgtcc cgacccagcg ggttcatcgt ggccttgccc aacaggttgc
     1801 cgcggaaata ccggttgtca ttctggaacg gcccgtcggt gtaataccct tccgctgcga
     1861 acagcgtccg tactgtatct ttggtcggcg aaaacatcag gagatggcgc tgggtgttga
     1921 actgtccacc ggccgactgg acgacgcctt ccttcaccat gtcacgcgtg cggaaattca
     1981 ccgcgccggc tgtagcgaaa tctccatact cgggcagata ggcccctttg tagacatcga
     2041 cgccttcaat ggtttccgga atgatgaagt tcagatcggt gtagccctgt ccatgggcat
     2101 gacttcgcaa attgatcggc atgccgtcag tgaagaatgc gacatcggtg ccatggtccg
     2161 catcaaaccc gcgcaggaag tactgatcgg cctttccggc cccacccgaa tgttcgaccg
     2221 caatgaatcc ggggatgagc cgcaagacct gggccggacg gccctgcggc tgcatgagat
     2281 attccttgtc gggaatgaac tgttgagagg acgctgccac cggccgatca gcagacactt
     2341 cgacttccgg cagctcgagg tccacggcat ccggatcatg ggcgtgaagc ggcgcagccc
     2401 acaggatcag agcgagccac agcacgacgt aacgcagcgc accagtcacc ggcggctcac
     2461 cgtagccctt tgccggtggt cgtcatggtc aacttgccat gcttcacgcc cttgacgctg
     2521 agcaacgcgt cggccacgtt gcggatggcc gaccctttgc cacgcgccac caacacctcg
     2581 aggcaatgat catggtccaa atgcacatgc atcccggcca tgatgtgccc ctcaaactcg
     2641 tgctgaatgt gggtcagctt ccgggcgagg tccggcacat gatggtcgta cacaaacgtg
     2701 atcgtcgcga ccgtatcctt gttctcatcc cactcctgac cgaccagact gtctcgaatg
     2761 agatcgcgaa tcgcctcaga gcgattcgtg tacttccgcc gttcgatcag gcgatcgaaa
     2821 tcgacgagca gatgacgatc gagtgacacg ccgaatcgcg cgagtgcgtc catgccgcct
     2881 ccttcaacgg tgctacaaat attttttttg taacactgcg atgaaagacg cgtcaatcac
     2941 gaggaggctg actgagaggg gctgcggacc gaagggtgac gatcgatatt tccggcggca
     3001 cgcgaaagcg aaccggcagg atactggtgc cgatgcccgt gctcacaaac aaatgccgcc
     3061 cctgttcgac gatatgccca atggcatacc gctctccgaa ttgagacggc acgacaggcc
     3121 gtcctaatcc cggcactgcc acctgcccgc catgtgtatg gccggcaatc gtcaaggtca
     3181 cacgggccgg aatctcggga aagacatccg ggttgtgcgt cacaacgatc accggacttg
     3241 cctcgtccac gccggtaagc gcgcggcgaa catcatgcgc cgcttcccaa aaatcaccga
     3301 caccggctaa ccagaacggc atccggttga acacgagcgg gatggcacgg tcctccagca
     3361 ccggaatgcc ttcggcttca agcgctttcc gcacacgcgg accatcaagc caccaatcgt
     3421 ggttgcctaa caccgcgtag actcccagcg tcgcctgcag cgagcgaagg acccgggcgc
     3481 tcgcttccgg ttcaacaaaa cgtccgccca ccacaccctg gatcacaaaa tcccccgcca
     3541 acaggatcag gtccggatgc gtgccattgg tggatgccac cacctcctgc aacttttcga
     3601 gatcgttatg aggtgagccg gtatgcagat ccgccaaaac cgctatccgc aggtcacccg
     3661 cagcggcagg ccatccgggt aagacgagat catattctcg ggtagacagc gatgcaggct
     3721 cccaccagaa gccccagcca gccaaggcaa ccaacagccc ggcgaggaga taccaaacgc
     3781 tgcgagcgtt ccatcgcctg acgcgcatgg ccattaagac cgcgacctca taaactggta
     3841 ccaatcgggt gatgtctctg ccgaccgaac gcagtcagcg tcgaacatcg ccatctcgtc
     3901 gcagtctctt ctcgcgaaca ggcgatcatg ggatgacgat gctcgtgtct gatcagacag
     3961 cctgatctgc cggtggggaa acgaggggac tatgcaaaca actgcacggc cgtcgagtcg
     4021 gccaggagac tctcgccttc acgatcgatg agcttgagaa agagggcgct caactccgga
     4081 tcaaactgcg tccctgcatg tcgctcgatc tcggccctgg cctccacgag attcagctgg
     4141 cctttgtacg aacgtggatg ggtcatgaca tcaaatgcat ccacgagcgc cacgatccga
     4201 ccgggcaagg gagtttgcgt ctccttcagg cgatgatatc cggtgccgtc ccatcgttca
     4261 tgatgcgtca acgcaatttc tcgtgccatc atcaagagcg gagaccggct atcggccaag
     4321 agtttcgacc cgatcttggt gtgcgtcttc attgcttccc actcaatcgc agtataggaa
     4381 ccttctttca tgacgatgga atccagcaga ccgatcttcc ccacatcatg aagcggagcg
     4441 gttttcttga tcatgtcggt ctgttcaagc ggcaatccct tcatctccga cagcagcatg
     4501 gccagacggc ccacgcgcca ggcatgggcc cccttgggat catagcggta ttcggaggcc
     4561 atgaccaagc gattgagcag atccagctgc gtctgttcca cgactttggt gcgcagccgg
     4621 acctgggctt caaggtgctc gttgtgcatc cgcaactgcc cgtgaagacg tctggtctgc
     4681 agctggttct tgatccgtaa caccacttcg gtggcgtcga cgggtttatt gatgaaatcg
     4741 ttggcccctt cggtgagcgc cttgtgtttc gtcggcgcgt ccaactcacc cgtcacgatc
     4801 aaaatcggga catagtcgtc ggcaggaatg acctgtctta actgtcgcat cacgtcaaac
     4861 ccatccatgt gcggcatgcg catatcgagg gcgacgagat ccggctccac ctgttggaac
     4921 agctccagcg cctgacgaga gtcattggtc atcgtgatgc ggctgaagcc ggcctgccgg
     4981 aggaaagcct ccatcagttt ggtgcaatgg gcttcatcat cgaccaacag cagtttgatg
     5041 gtgctcagat caacgtcttg catcgcgctc acaccgctcc tctgtccgca gactcctacg
     5101 gtcgagatta ccaccgtcag gccgcatcgc caatttcttt tctgctctcc gcagggagcg
     5161 aaccggggaa cgtgagagtc gacaaggtcc gctggagcgc cgccatggtc aacggcttgc
     5221 tcaactcggc atcgacggct tgggaagcgg tctccgtgac ctcaatctcg tcgccgcccg
     5281 tcagcagcac caccggcgtg tccggcgaca cctgtttgat gacttcggcc aactgtcgcc
     5341 cggtcatgtt cggcataccg tgatcggtca cgaccaggtg cacccgctcg gggttgaacc
     5401 gcttcaccgc ttcggccgca ctggtaacga cctcgacctg atggccggcc acccgcagat
     5461 attcgccggt cacacgaccc accagcggat catcatcgac gacaagtatc tgcagccgca
     5521 ccccggccag gctgttgcct tcctgtttgg tgcccgcctc ctgctcggat tgaatcggca
     5581 aacggatgct gaaagtcgtc cccaccccga cggcactggt gatctcgatc gtcccgcgca
     5641 gccgtttgat gatcccatac accattgcca acccgaggcc ggaccccttt tcgcccttgg
     5701 tggaaaaaaa cggctccaga caacgctgac ggacctcctc ggtcatgccc gtgccggtat
     5761 cgctgacttc aagacgcaca tggtcgccat ccgccttcgt ccgcagcgtg atcgacccgc
     5821 tcttcggcat ggcatccacg gcgttgaaga cgagattcgt cagcacttcc cgcaattccg
     5881 attcgtgtcc ggccaccgga ggcaccgaag ccaattccgt tttgatctcg atggccacgc
     5941 cgttcgccaa ggcctgatcc ttccatttcg gctggctgag cttcacactc tgttcgacga
     6001 gtcgattgag atccacgatg ccggccaggt ccgcatcgag gcggggccga taaaattcgc
     6061 gcagacggct gacgactttc gccgcatcct tggcggccat attgatggtc tggagatatt
     6121 ccttgagttg cgcgggatcg gccgacatct gcggcgcaat cagcaacagt tcgctgaacc
     6181 ccataatggg cgacagcgtg ttattgaaat catgcgcgat gccgctcgcc atttggccta
     6241 aggcgcgaag ccgttcctgc tgcaccattt gctgctgtgc ggtctgcagg tcggtcatgg
     6301 cctgctcgag ccgcgtatgg ctttcacgca gcgaggcctc tgcacgcttc cgctccgcca
     6361 gatcagaagc attactcagc gccaccgcca cctgatcggc caactgacgc aattgcgtct
     6421 tgtgctcgga atccatctcc ggtgcctggc ggtaactcat cgcgattgca cccagcagat
     6481 cgcgtgagcg gaacaagggc agcaccatca gagccgtcac gcccggtcta cagagggtgg
     6541 caaacgcagc ggctccatcc accccgatca gcgcggtgcc gccttcgtgg gaggccagca
     6601 tccggcgctc ttcttcgccc acagacacgc cggtcatcgt cgtcggcttg ttcttcgccc
     6661 ccgatctgac atagagccag cctcgcgccg gatcggacga atccatcagc agcatggcga
     6721 tcccgtcgca aggcgacacc tcacgcaccc gggtcaatac cgtatccaca atgcgactgg
     6781 gatcgagcac cgacagcacc gagcgatcaa tctcatggat cgtggccaac tgggtgaact
     6841 gttgattcaa ccgaccggcc atcgcgttga acgacgaggc tagcaattcg aactcatcgc
     6901 cgctccgcac ctgcaccggc gtcgaaaaat ttcgcgcagc gattcgcctg gtgccctcgc
     6961 tcagttgttc gagcgggccc atgaactttc gaatctgaac cacaccgagg agggctgagg
     7021 caaacaccgc cgcgatgata gccaggcgga cctgacggcg gaggtcgctc gcctgatcca
     7081 acaccacact catgggttta ctcagcacca ccacccagga atccgtcgag aatcttgacc
     7141 tgagcgggat gccccaggcc cgcgccagct tctcgtcatc gccatctcgc catgtgaaac
     7201 ttccggagct ggctttgccc tgcgctagcc actgctccga cagcgctacg gacgcagcat
     7261 cggtgcaaag gagcgtttgc ccagcctggg cgaaaacaca cagtgaagtg tcgggcggtg
     7321 gcgcaagact atcgcccaca tcccataaaa agtcgtgctt gatttccgcg accaccactc
     7381 ggccatcttg ctccgacatg gtggaaagaa aaaaacgagg tcggtcaaca ccagcgggtt
     7441 cgaccgtgag gagcgtcttg ttctgacgaa gtatcgcacg ctccgcagag ctgaaagcgc
     7501 gggaccgctg cccaccacca aacaccgccg ttccgtcggc ttccgagaca gaggccatgc
     7561 cgatgaatcc cgaacggctc gatagcggcc gcgagggcca ctcttcggac cgcccggaag
     7621 caccgacctg ctgtcgtgcc gtgatcgcaa gcaattcctc ctccaatagc agcaaccgct
     7681 ggtacacggc catgcccatg gccctgcttt gctgccgcat gtgttcttcg gcctgcgtcc
     7741 tgaggtattt cgtcacttga gggatcgtga cgacgctcaa cacgccaacg gggagtagtg
     7801 cacagaggat gaacaggaca aagacgcgcc ggcccagacg agtgcggagg aaagaatatt
     7861 cgatctgcac aatagcttcg tctcgttaaa actcaacggc cagcccaaca aagctgccgt
     7921 cattcgcgcg gatcacgtcg tcgtgacttt tcttggctgt gagcggttcc acactctcac
     7981 catcttttcc catgctatac aggtcgtagt cggagttgat tgggtgcagg aagcgatcct
     8041 ttctcggttt gcccttgctc gcattccccg atccgttgcc gttgccattc ccgtttgcat
     8101 ttccggctgc gtaggcctcg acggggagga gccagtccca ggcaccgtgg tttccaccac
     8161 ccccgccatt tccattgtta ctgggtgggg tcgttgcgtt tgtgcccccg gttcctccag
     8221 aactactccc agtacctcct ccaccgcttc cgcccggagt atttccattg cctccaccac
     8281 tgctcccatt tcctcctgac ccgccacctc caccgtttcc gccagcattc ccgttaccac
     8341 caccattccc tggcaaactc aaggcggcaa tgttcagata ctcgtaggga ttcccccagg
     8401 ggtcgacgat ctcgccggct cctgcttcgg ccaatgtgtt gggatagaca tcataggcgg
     8461 cgttgtagga atcaaccgcg cgggagatgt aacgaatttc ggcaatgcag cgcgcgagcc
     8521 tggctttatc caagtacccc acgtacatcg gcacggccaa cccggccaga gcgccgacaa
     8581 tcaccatcac aatggccagt tcgataatgg tgaatccctg ctggcgacga tcgaagggaa
     8641 tgtgaggcat aacgatccta gaatgcagag caaaaactcg cttattgcgc aacacgctac
     8701 agctcactgc cgtgccgcct cgctttggct gtcacccgca ggatgcgcaa aaagtccgtc
     8761 cagcaaggcc gcagcaaacg aagaggcgaa tcgtactcgc cccgtacggt gagcctctga
     8821 gtgccgcgag aacgccgctg gcggactttt tccgcatcct gctagaatcc tgcagcggtg
     8881 ggcaaaggcg tgggacgcgg ctcaaccggt tcagaggtgg tctggaacgg ctccatcgcc
     8941 tgcgccagtt gttcgacagt ggcgcgcaat cgatcagcct cctgctccac ttcgcgtaag
     9001 gcaccacgag cggccgcggc cttcagccgc tccaactgcg gggccatatc ggcatggtgc
     9061 gcctcgatcc ggccgatctt ctcccgcaac gcactgacca tttctcccaa acactgggct
     9121 tcggcatgca gatagtcgct cttgcgaatc gaggtgcgca cggtcagatc cccgttcgcc
     9181 acggactgga aaatccggcg gaagcgatag agcgggccgg cgatgcggtg cgtgaaaaat
     9241 actccgtgga cgatcaacaa taacgacacc accccgacgg ccggccagaa ccggctgtgc
     9301 aacagctcaa actgatcggc cactcgctgc gcctcaaccg aggacagggg caggtgatcc
     9361 agttgcaaca tgagcggaag aaacaacgcg ccggagacgg ccgcgatgac caccaccacg
     9421 tagaggagac tcaggcccac aaacctcggc tggaccgtgt cccacaaaaa atgccgtctg
     9481 aaacgtggcc tgctcatgtg gtcgccgccc ttttggtcca aaccaaatga tcctggtttt
     9541 taaacgtgat ccgtccgcct ccggcgggat cgtaataaaa cgacaattgg tacttcgcct
     9601 gctcggcatc ctgataggac acgacgaaga gcgttctggc cccatcgcga ccttcgtcga
     9661 ccttcgtaat catgtagccg tccgccgcga gcgcgctggt cccgaatccg atccgggttt
     9721 ggtcaatgaa catatgttga tcgacatacc catcggtggt ggtttcccac acgccgatca
     9781 gatacgacgg agcctgatcc ggttcaggag cacaagcggc acaccaccac agcaccagca
     9841 gacaactcac cgctctgatc gaccacacaa gtcacctctt cccatggggt gtcggcgaaa
     9901 tcgactcgac acggaatcag tatgtcgcag ttgccgggaa atacaatttc tttaaaagtt
     9961 cctacacgca gggcggaggg tcagaaaaac agcgtcgggc cgccggtagg tgccagcaac
    10021 gagagcggaa gggtcagaac acgactattc ggtttggatc gggatgtaga gcaaactgcc
    10081 ctgccggttg atgagcagca gagcgagatc cgttgaacga agcggctcgg cgagccgttg
    10141 aaaggtgctg aagttgggaa tgggctgtcg attgagttcc agcagcagat cacctggttg
    10201 caggcccgat acttccgcca ggctgccgtc ttcgatatcc gtcaccacca ggccggtgtt
    10261 ggaagggaga tccatctggc gcgccagcgc gggagtgatt tcatccacga tcacacctga
    10321 aagaggatgc gctgtgggac cagccgtctc ggcagactgc gtcttccgct gccgttcgcg
    10381 cggcgcctcc tgcacggtca attcggcttg catcaagcgg gcatcccgca ggagttcgac
    10441 ccggtgtttg ctgccgatgg cggcggccgc catcagattg cgcagatggc cgctatccat
    10501 gacatcacgc ccgtcaaacc gcaccaccac gtcgccgcgc ttcaacccgg cacgttcggc
    10561 agagcccttg gcctgcagat cggtaatgat cgaccccttc acgtccggca ggtgaaaaat
    10621 cttcgccagc aagggcgtca catcctgggt cgatgcgccc agaaatcctc gcaccacacg
    10681 gcctgttttg atgagactct gcatggcggt cctggccatg ttgctgggaa tcgcaaaacc
    10741 cacgccgacg ctcccgcccg taggactggc gatggcggtg ttgatcccga ccaactctcc
    10801 attggtattg accaaggcgc cacccgaatt gccgggattg atcggcgcat cggtctgaat
    10861 gaaatcttcc acatcggcga cacccacatc ggcccgtccg actgcgctca cgatgccgaa
    10921 ggtcaccgtg cggctcaggc cgagcgggtt tccgatcgcc agaacaaaat caccgacagc
    10981 gagcgcactg gaatcgcccc aggctgcagt gggaagcccg ctcgcattga tttttacgac
    11041 ggccacatcc gtcttcggat cggtcgcgac gacgcgccct ttgaactgcc ggcggtcggc
    11101 caggatgact tccacgtcca ccgcatcggc caccacgtga ttgttcgtga tgatataccc
    11161 gtccgacgag acgatgaccc cggatccctg cccgtattgg cggcgaggcg gcacatcctt
    11221 aaacatgccg aacggcaggc cttcatcgct gaacccctga tcgtgcacca tgacggtgga
    11281 cgcaatgctg accacggcag gaagaacctt ggcggcggta gcccgcacct gcgcctgcaa
    11341 atcgccgttg gtgctcacct tggagaaacg gggcgcaagc atatcagccg ctgaggcagg
    11401 tccttccggc caacagccca gcagcgcgcc tacgattccc agagccacca ggcccggctg
    11461 tgatcgacgt accgacatgg tgtgacacat cctcaagacg gcagggccca ccctatcacg
    11521 cacagggttc acgcacacgc agaactcata aatcggtctg ccgcaagcgt agcgcgttcg
    11581 aaatgacgga gagcgaactc cctgtcatcg cagcactggc aatcatcggg ctcaggagaa
    11641 gaccaaacac gggatagaag agcccggccg cgatgggcac accaatacta ttgtacacaa
    11701 aggcgaagaa gagattttgt cggatgttcc tcatcgtggc cttgctcaag cgataggccc
    11761 ggacgattcc ccgcagatca cccttcacga gcgtgactcc ggcattttcc atggcgacat
    11821 cggtgccggt tcccatcgcg atcccgacat cggcctgggc caaggcaggc gcatcgttga
    11881 tgccatctcc cgccatcgcc acgacctgac cttgccgctg cagctcctgc acgatccggc
    11941 tcttgtcttc cggtttgacg ccggcccgca cctcatccag acccaattcc ttcgccactg
    12001 cctgtgctgt aacggcatgg tctccggtga ccatgaccag gcgaatgcct tcctgcctca
    12061 acaagcgcag cgcctcaggg gtggagctct tgatcgggtc ggccacgccc agcaatccga
    12121 tcgcctggcc atccaccgcc accagcatca cagtctgtcc ggtctgccgc atcaattcgg
    12181 cattggcatc caacgcggca agggctgctt cgtcgcccac cttcaggtcc cgcaaccagt
    12241 cgatagtccc tacggcaact cgcttcgtcc cgacggtcgc catgacgcct ttccccgcct
    12301 tcgaggtgaa cccggccgcc ggctctaaat tcaaccctcg cgcttcggcc ccgccgacaa
    12361 tcgcattggc gaccggatgc tcactgcccc gctcgaccga cgcggccaat cgcaacagat
    12421 ccgtttccga ccagggtggc atcgcactga ctacccgcaa ctttggcttg ccttccgtca
    12481 acgtcccggt cttgtcgaac accagcgtgg tgaccttttc gatcgtctcc aacgactcag
    12541 cctttttgaa caagactccg gccgacgcac cccgccccgt ccccaccatg atagacatgg
    12601 gcgtggccag ccccagcgcg caggggcagg caatgattaa cactgccacg gcattcaaca
    12661 gggcatgggc tagacgaggc tccggtccca gccacgccca tagaatgccg gtgacgagcg
    12721 ccacgctgac aacgactgga acaaaatacc cggccaccac atcggctgtg cgctgaatag
    12781 gcgcacgact gcgctgagcc tcggccacca tttgcacgat gtgggacaac agcgtatccg
    12841 cgcccacccg atcggctcgc atcaccacgc cacccgatcc gttgatggtg ccaccggtga
    12901 cccgctcacc ggcaaacttt tcgacgggga tcgattcgcc agtaatcatg gactcatcaa
    12961 tcgaggtcgc cccttcgaga atcactccat ccaccggtac ccgttcacca ggacgaaccc
    13021 gcaaccgttg gccgacctgc acctgttcca gcgggacatc ttcttcacgg ccatcatcga
    13081 acaaccggcg ggcggtcttc ggggccaagc caagcagcgc cctgatcgct ccggtcgtcc
    13141 gactcctggc ccgcagctcc aacacctggc ccaagagcac caggacggtg atcaccgcgg
    13201 ccgcttcgaa atagaccggc accgcaccgc tctcgagatg gaaggattgc ggaatcgaaa
    13261 acggaaatag cgtcgcgatc acactgtaga gataggctgt cccggtgccg atcgcgatca
    13321 gagtgaacat gttcggactg cggtgcacaa tcgacgccca accccgctga aagaacggcc
    13381 agccggccca cagcaccacc ggcgtgctga gcacgaactg cacccaggcc gactgggcat
    13441 ccgacagaag atgttggatc ggatggccgg gaagcatgtg cgacatggcc aacagaaaca
    13501 cgatcgccgc cggtcccaat gccacccaga accgtctggc catatcgacc agctcgggat
    13561 tcagttcctc cgcgacagtg accgttcgtg gttccagcgc catgccgcac ttcgggcagg
    13621 cacccggctc cgcctgcacc acctccggat gcatcgggca gacatattcc gtcttggtcg
    13681 gcagcggttg gacggaatcc ggttccagag ccatgccgca tttcgggcaa gggaccggct
    13741 tctcctccaa tacttccgga cacatggggc agacatactt ggttcccgga gggaccgggg
    13801 ttgcaggcat cgcatctttg acggagcccg gcgataggta gcgcgccgga tcggcgcgga
    13861 acttggtgag acaaccttgg cagcaaaaga aataggtcgt gtcttggtag gcgaaggaac
    13921 cagccgccgt cgccggatcc accgtcatgc cgcagaccgg gtcgatgaca gccgccgcgg
    13981 agggcccggg ggacgaggcg gcccccaaca tgggtaaggc ctttcctccg aactgacgca
    14041 gcgatgtcga tgcaggagcg gcgacgggag ccaggtaccg acccggatcg gcccggaact
    14101 tttcgagaca gccctggcaa cagaaataat aagtcttctg ttcatgggta tgagagccag
    14161 ccgccgtggc cggctcaacc atcatcccgc agaccgggtc ccgttcgccg cctgtggtcg
    14221 cttcttgcgc aggcatcgcc tgcatcatcg gtagcgagcg gcgactggat ggttggacgg
    14281 cggcaccggc gggggcggcc ttcttcacgt agtgcgccgg cgcggcgcga aaccggtccc
    14341 ggcatgaggg gctgcaaaaa tgataggtct cgccctcata ttccgcgtac cctgccgccg
    14401 tggccggctc taccgtcatc ccgcatactg gatcacgtgc cattcgcgct tgtacctcgt
    14461 cgttcgtcgc tcgtatctcg tcgctcgtcg gttccgtctt acgtttcacc tttcacgttt
    14521 cacgaaatac gcttcacgag atacggcctc gttacttttt gctgtctccg tccaggatgc
    14581 tttggatgat cagcttcaaa ttctccgggt cgatttgctc caccttgatg ttgccgtcga
    14641 gcaacacggt cggagtctgc tggatcttga tgcgatcgcc ccatttccgc ccgtcttcga
    14701 acgccttcgc cggtttcgca ctggccagcc cttcttcgaa ggccgccgga ttcagtccga
    14761 cctcgcgaat caagacttca cgaatcgcct tgtcgatgat gcctaccttg tctttgtgaa
    14821 tggtccggaa cagcgcccgc ttcatttcat tgcccttgcc catgctcttg gcctgctcat
    14881 acatatcgaa ggccgtcggc aacttgccgg ggatgacggg atagcccacc atgaccgctt
    14941 cgactttgtt tccgaattcc ttctccagaa tcatcagccc ttcgccatcg aaccggtggc
    15001 aatgcggaca atagaagtcg gcgaactcca ccaactggac cttacccggc ttgtgggtcg
    15061 atttttcttc gctcaagacc tggaaattac ccttgagttc gggcttcgcc gcttgcgctg
    15121 tgcccgccaa tcccacaagc gccatcacca tcacgcccac gaacagacca ctgtgccaca
    15181 cacgacgcat caccatacgc tgcactcctt tttcaaacat gaatcgttgc cacaggctcc
    15241 tcggcattat aaacaatgct ggcactcttc tgcgtctgtt ataatgccaa cgatgcgtgg
    15301 gaaacttcta cagggtctcg gtattttgtg catgaccgcg atcctggccg cttgcgcgac
    15361 ctccgccgat cagcgtgaag ccgaagacgg cgcatcccag acgccgcaat tttcgcaaat
    15421 caaagctgcc ccggaatcgt ttaaaggaca gaccgtcgtg ttgggcggcc aagtcctctc
    15481 ggcccggcgg atgaaagacg gaacccgcct cgaagtgctt cagttgccgc tcaatgacgc
    15541 gcaacagcca tctctggatc tgaccaaatc gcagggccga ttcattgcga ttcgccggga
    15601 attcctggat cctgcgaccg tgccgcaggg cacctttgtc accattacgg gccagatgac
    15661 gggctccgtc actcagccgc tggatgagac cgactacgct tacccggtgc tggagatcac
    15721 cacgtttcgc acgtggattc cttctcagga tttgaacctt cctcggtacc gcccctaccc
    15781 ctattacagt ccgttctggc atccctattg gggaccgtac ggacgttttc cttactattg
    15841 gtagcaccgt cacccgcact ctcgcctgga atcatcgccc gttttctgcg ccttgtcgga
    15901 ccgcatgctc gagctgatcg tggcttacgg ccctgaacga caacgccccg tccatccgga
    15961 gatgaacggg gcgctgcgag aaccgaattg attcggtgtt tacttgatca tgatcaactg
    16021 gccgccgggc tgcttcgggt tcaattgatc ccgataggtc agggtgttgc cctgggtggc
    16081 gttgaacaaa tcgctggtcg gcacaccgat gtacttcgtc tcaccgggct tgaggaccac
    16141 ttcagccttc agcacgaacg gagaggctgc gttgaccggg tccgtcatct ggaagccacg
    16201 gtcagaagac gtgttgttcg tcaccttcag caagaccgga cggccgggac ggactttgaa
    16261 gtcgatgatg gtggtcggtg ggtaccacgc cttcaggccg cccacttcga ccgcaaacaa
    16321 ctgcgcatcc acttccaact ccttgaacgg ttggccgacc acagaaccag tctcaaggtc
    16381 gcccacctcg acgccgcccg cctgcaacgc ccaggctccc gtggcgctcg acaacagact
    16441 caacgcacca aacaatacca ctgcactcct gctcaacata cacaacctcc tgtcgggtta
    16501 aagagatgaa gacgaacgat gctccggagc caggcacggg ggaagaccag tgccttccag
    16561 cacctgaccg ccaacaatga gaccatccac gatgagaaac aacaccttca tgcgcttcgt
    16621 gctgtctgaa aatttgccgc tggcgatggc cagcacttca tgcgatccgt cgggctgagg
    16681 atgatctgct tcgatgttga cataatagaa atcaaatccc tcgaactcct tattgaacgc
    16741 ctgggcccgc aacagatgct ctagcgagtc gtcggccccc catcccgcgg ttccaccggc
    16801 caccaccaga agttggatca ggacgataca gacacatcgc atgaggctac ctccctaggg
    16861 tgggagttat agcctcgagg cacccggtcg tctacagacg cacgctcatg gatgcttacg
    16921 ggtgctacag tatcgtttcc ggaccaccaa cggcaagagg ttatacagga ttacggcgcc
    16981 tgagggaacc aggggaagac cgagggtaga actggaggct ctggcaggaa gccacggggg
    17041 aagcggaatc cttgatgctt cccaccgtag cctcatcgtt cggttagttg ccctttcctt
    17101 tgcctttttt gcccgaggcc tgttcggcat gggccaggga ttcttccgca tgcttcacag
    17161 cttctttggc atgctctaac gcctccttcg catgcggatc gttgctgccc ttcaccgctt
    17221 cttccaagtg cttgatcgct tccttctcat gctccacgcc ttccttggca tgcttgatcg
    17281 tttctttcac atgcccgccg ccgtcggcca gcgccatgtt tgccggcgcg accgccacga
    17341 gcgcacatag agacgcaacc actactcctg cacgtaatcc tgtcatacgc attcctcctg
    17401 tgtcatagtg aaccgttggc tgattggccg cattctacag cattttgtag acggtgttga
    17461 gcgagatggt ggtattgaga gtggtcaccg ggaaagaaaa aggtgggaga gggaagcgta
    17521 tgggcgtacg cctccctcat cggagctcac tgcgtggtca gcgcagtcca accgacgagc
    17581 tccgtccacc gatcgtcagg cagaggccgg ggtgagccac ggcaaacggc agatgccaca
    17641 ccgcccggga tccgctctcg ccgactggcc gccggaatcc cagctcgcat agatccacaa
    17701 ttcagactgt gccgtcccga cctcgtctct cgtcatcccc gcgcattttt ccatgacaaa
    17761 caccgctgga cgagattcct gcgcgccgat tcctaccgga cagggttcgg attccaccac
    17821 gaacaccgtc cccacgggaa atgatcgatc atccgccgtg aggagcccct tcgggcacac
    17881 gtaaaaccgg agctcttgcg ctccgccaga cgactgcaca agcgacgtaa gatggggcca
    17941 gtctcgatac ccgaacggga tcatcaatcc ggctgcgggc ggagccggcg accaccctcc
    18001 gctgatgtcc aacgccagcg ccaacaagcg caccaccgct acattcaatc ctgtctggtc
    18061 gtacggcttc ccggcgaccg tcgttcgttg aggagatgcc accgcgtcac tgaccggcat
    18121 ggaccacctt cctttctgat cggggcacag gttatcgact ggcttctccg accgtcgcgc
    18181 caaggtaaga gaccgcgccc tgcttcaccg ctccgggctg agaccgtgcg cgaatcgatg
    18241 cggcgcgcgc cgcatatagt ccggcctcca ccgattggcc gatgctgttg agtaccactg
    18301 catatcgttc caccaggagt ccaaccgtgg ggtggtgcac cccgagctgc tgttgctgaa
    18361 tcaccagcgc gcgctgatac cacagttgcg cctgagccat cagtccctgt tgatcggcca
    18421 ggaacgccat gttgcctata atcgtgccgg ccagagcgct ctgagggccg gagagccgct
    18481 cggcgagcgg caaggcttcg tcgaaatggc gttgcgccga ggcatacagc ccctcggctt
    18541 ggtacaggca ggccaggttg ttcaaactca tcaccacagc cggatggtcg ttgccgaagc
    18601 gaagccgacg gaccttcaag gcccgttcgt acaacggttc ggcccaggca tacatcccgc
    18661 ggcccttgta taacgcagcc aggttattga gcaccggccc cagctcaaca tgatcggggc
    18721 catgcgcctg ctccatcagc gtgagcgcca atcgatacgt cgattcggca gccgtctctc
    18781 gcccctgctt gcggtagagc tcggccagat tgttcaagct gatcgcgaca tcgggatgcg
    18841 ccgggcccag cgtctggcgt tcgatcttca gcactcgctc atacaatggt tccgccgcag
    18901 cccactcgcc tttggcatgg gcgatttccg ccagattgtt caatgcggcg gcgacgtctt
    18961 cgtgctccgg ccccagttga gcctcccacg tgccgagcgc gcgcagatag agtgcttcgg
    19021 cttctgagag ccggccctgg tcgtgataca acaatcccag ctggttcgcg gccagcgcca
    19081 ctcgcgcgtc atcggcgcca ccctgttcgg cttcccgcaa gacctgcatc agaatcggtt
    19141 cagcctccgc gtaccgcgcc gcttccctgg ctttcacgcc tgcctcgaag cgctgcatcc
    19201 agccatccgg ttccgtcgcg ccgctccctg tgacggcgca taagactatc agcgcgacca
    19261 ggctcacgaa tcccattcga taccaccgcc ggttcatgcg atcactcatg atggtcgagt
    19321 cctccacacc ttcgatcgac tccgggctca ttccgtcgtt cccgttcctc gcgcctgttt
    19381 ttaccttgcc agcccccgaa gggccggggt gaagggggag gccgtcccat tcaccccggg
    19441 gaggcag
//
LOCUS       DAOQEW010000057        19414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_715_length_19414_cov_7.795341, whole genome shotgun sequence.
ACCESSION   DAOQEW010000057 DAOQEW010000000
VERSION     DAOQEW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_715_length_19414_cov_7.795341"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            633..896
                     /locus_tag="PLS35_08225"
     CDS             633..896
                     /locus_tag="PLS35_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18685.1"
                     /translation="MPQQHMDDHSGQSAVQLHVPAARAPRDLTYDEKKAAEAAFRGEP
                     FNPAWSAAAAVVYEGIVSAMCRMQVASLTDMEAVSVAEECLTR"
     gene            complement(935..1552)
                     /gene="thpR"
                     /locus_tag="PLS35_08230"
     CDS             complement(935..1552)
                     /gene="thpR"
                     /locus_tag="PLS35_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNI18686.1"
                     /translation="MIRAFLAVELPPDFRAALSVIQQDLKRRLEPAVGRQARVSWVQP
                     ASMHLTVRFLGETPQEVIESLHAVVSEVVANHHMIEVPFSRLGTFPRIQQPRVLWAGP
                     LESWEQGSHGQRLQVLYRAVEEVSREAGLAAEVRPFSPHLTLARIKEGERQVGQVLAQ
                     SGALDQPVTIGVLPIKALVMMRSELHPAGPVYSKLWECPLAASAA"
     gene            complement(1549..2832)
                     /locus_tag="PLS35_08235"
     CDS             complement(1549..2832)
                     /locus_tag="PLS35_08235"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNI18687.1"
                     /translation="MNKSKAWTAETIAIGSELLLGGRMDTNSLFIADHLATCGVELRY
                     KSIVGDELSDIVGVLKTAARRVRVVIITGGLGPTIDDLTREAVAQATGQRLSRRKTAL
                     DGMIARLAEWGRTPTKAQLRQAMIPAGGEVLTNPVGTAPGFALIWKDTFFAALPGVPR
                     EMEPMIQDGVLPRLQAWIGKHKGIPATPVIRRVLHTSGVPESIVDQRLEGLLPDGTAI
                     QLGIYASPMEVMVSLTGPEKESGPVTIDRLFEDAKTRLGDIMYGEADETMETVVGRLL
                     TDQHLTLALAESCTGGLIGHRLTQIPGSSAYVDRGVVSYSNRAKVELLGVSQDLLDRH
                     GAVSAEVAAAMARGIRERSGVSVGLSVTGIAGPGGATKSKPVGLVYVGLDGGPGENLT
                     KEFRFHGGDRSVIKQRSSQAALDVLRRWLITKGGG"
     gene            complement(2879..3493)
                     /locus_tag="PLS35_08240"
     CDS             complement(2879..3493)
                     /locus_tag="PLS35_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18688.1"
                     /translation="MRTIRRRCAGFKLALYLFVVAGTGPPALSLAIEVQPTPEQIQAT
                     MERGKTAAAKGASPDQLHEWFGAKEDLTPHGFIMTKLGSLLVMANHLSLRALSPTDQD
                     VAQVMANGNLLVNVVLFGDRVNFAADSYIVLEQAGRTVKPSSVRFDARGERTSVWPQQ
                     PAYRAKVIAQFPYADLDPHAKTTLSVFPSSGGQVTFELDFAHIE"
     gene            3639..4346
                     /locus_tag="PLS35_08245"
     CDS             3639..4346
                     /locus_tag="PLS35_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNI18689.1"
                     /translation="MSSPEQAPRRQYVNFTFYKVDPAWRRLPEDERTRGKQEFLRAVE
                     EYQGKVLVIAYTSVGIRGDCDLMLWRISYELELFQEMSTKILASGLGKYILNPYSYLA
                     ITKRSIYVDNHTHENQESKRLTVVPGKAKYIFVYPFVKTREWFLLTKAARQGMMDEHI
                     EVGHRFPSVKLNTTYSFGLDDQEWVVAFESDKPEDFLDLVMALRETEGSRYTLRDTPI
                     FTCIRKSLKEALDTLGG"
     gene            4455..5117
                     /locus_tag="PLS35_08250"
     CDS             4455..5117
                     /locus_tag="PLS35_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18690.1"
                     /translation="MSVEAQTHPASDYRAAVYSAILPGLGHLLRGRHKAAMFFGFITI
                     LLIILSLGLGRVSGRAAEVFFFMLLALPWWALQSYDAALGPAASGSDLVRTGRLAWAE
                     GHDIRFLGLLFLVSAANDAIIIAKNPDYLLPFFCTKLDGAAGFVTKALSPFLHTWVGY
                     GFLRIKKWSLLVYLVYAAYGTTNALVNLTCFGPGRIRNTLLIALIAFTSYILWRRRMF
                     QR"
     gene            5221..5433
                     /locus_tag="PLS35_08255"
     CDS             5221..5433
                     /locus_tag="PLS35_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18691.1"
                     /translation="MAENNAVYAIRHPDGSVTLYIDEEYAIDRGVDPAKLVRVEIPRE
                     LFVSGSIQHIREYVAVYLENSHQGTA"
     gene            5466..6635
                     /locus_tag="PLS35_08260"
     CDS             5466..6635
                     /locus_tag="PLS35_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18692.1"
                     /translation="MSALLTSELIASTIEQAPIQGRVMLKLLLLQHFDITPEEISYIA
                     ADRPDPRCVAGSKPIHQMVKQDALRDVTNRRDEYRRRVRLRRERLWLQMEAMKGMIAL
                     AEGMIRAATDLLRTRFKLDAEAVNAITQSARTAVPKPAIRSLNRRWDEDEISAESYQE
                     ARLGLEIQAQLRLIEKYRKRLEVSEREWKSINAAPLQDHEIGHIWGIPAGSLAGRKVK
                     HLHQYLHAIQSALQKASAQTVPTTGALDLWKETLAVLGERPVERSIALYDGLERTEEA
                     LVEKLRTFAWGTLGEDLEAKFWLSLVHGASSNAVHSENTRSVFGLQRLLAVLSEIDSS
                     PDALEEELLARTVATPKDVPALTDEAAQPKAEEMGQMQQHVLTSFIGELHNDLQR"
     gene            6738..7193
                     /locus_tag="PLS35_08265"
     CDS             6738..7193
                     /locus_tag="PLS35_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18693.1"
                     /translation="MYNPPIMQHDHVMIPVRSLARVAGLMLCMLVLPLSIMPVASLFA
                     AGLVEVTELLAHPDHYNHQVVTVSGRVSSFQLATNRDGQPAFGFLLQDTAGTVKVVGL
                     GKADVREGDYVVVEGIFSRLRQAGRAIVYNEIKATSVQSMAKMNPDLVG"
     gene            complement(7190..7666)
                     /locus_tag="PLS35_08270"
     CDS             complement(7190..7666)
                     /locus_tag="PLS35_08270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI18694.1"
                     /translation="MHVTAGDVVSVEYVIRLEDEQVIDTTVGEAPLVYTHGQHEILRG
                     LEAGLEGMAIGAAKRVTVMPVDGYGERHPEGLFEVRRDRVPVEAQRVGAKLETTAPDG
                     RVVFPYVAEIKPEVIVLDLNHPLAGKTLVFDVRVIDIEREGNRVVEEIAPAKKGES"
     gene            complement(7904..8470)
                     /locus_tag="PLS35_08275"
     CDS             complement(7904..8470)
                     /locus_tag="PLS35_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNI18695.1"
                     /translation="MPSEVLSVSSRRNFLRTVTVGTALFVANLALPAKARASRLPTGR
                     ISLFNLQTEERLSVTFRTESGTYDQSALDDLNYFLRCHHSNETTTMDVQLIEFVTLVQ
                     QKVGGRRDIHIVSAYRSPEYNEQLIRMGTRAARHSYHVSGQAVDVQIPGVPLRTVREV
                     ALRLGCGGVGYYPRGKFVHLDSGPFRHW"
     gene            complement(8781..10193)
                     /locus_tag="PLS35_08280"
     CDS             complement(8781..10193)
                     /locus_tag="PLS35_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI18696.1"
                     /translation="MAKHSVVIVGPDQSTVSAAQALIQDEFEVSTAMWTTDTLLPIHE
                     QASPVMLLDIDSPSQPSAEEGLNVLQKLRQTGYSGKVIAYTGRPERSVAVRAVQCGAC
                     DVLAKSLDPVQLLQSIERVARVADLEQEARGAMVVGGPEEFSGMLGMSASINRIFDAI
                     RKVSTSDAPILITGESGTGKELTARAIHARGLRKQGPFIPINCGAIPESLLESELFGY
                     ERGAFTGAVGQKKGKVEFAQGGTLFLDEVGELPNALQVKLLRFLQDHTFERVGGHQAI
                     EMNVRIIAATNVNLKEAIEKGTFRDDLYYRLGVVHINLPPLRERGEDVSLIAMAFLRQ
                     AAAHYNKPLYGFTREALEAMQAYSWPGNVRELSNRIGRAVVMAEGTHITAADLDLPRQ
                     APPQDDGSISLKVNQQRIETDLIMKAFTLSQGNLSRAAQELGISRSTLYRRLRQYGME
                     RSLEARRFPGLSPRASMTEH"
     gene            complement(10304..10696)
                     /locus_tag="PLS35_08285"
     CDS             complement(10304..10696)
                     /locus_tag="PLS35_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18697.1"
                     /translation="MGQSSTGGNGARSGKRPTVAVIYEDVTSGYRYADWFALHGYQAT
                     MTSAVPHLEHHLQELRPDVIVVGLSSCSTPLQTAVPRLKRTCPQVPIIAMLDRTAQDA
                     NGTLPALAKAFGGDVFMCDSLDPPSTVS"
     gene            complement(11087..11734)
                     /locus_tag="PLS35_08290"
     CDS             complement(11087..11734)
                     /locus_tag="PLS35_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI18698.1"
                     /translation="MRNTATVRILVVDDHPSFRRGVKDILEEGFEGASMAECGNAQEM
                     LDQVREQTYDLVVMDISMPGRSGPEVLKELKQLAPTLPVLILSMHPEDQYAIRMFKAG
                     AAGYLTKASAPEELVHAAKKVMAGGQYVSASVGEALALTVRTGVDKLPHERLSDREYE
                     VLCLIASGQTVSDIAESIHLSVTTISTYRARILDKMNLKNNAELTRYALQHGLVV"
     gene            complement(11753..14905)
                     /locus_tag="PLS35_08295"
     CDS             complement(11753..14905)
                     /locus_tag="PLS35_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18699.1"
                     /translation="MYSLARFSLLEMTECSAVLRQLGEQSRSLQEAASKAVNYLFDTL
                     GDPTTGQRDCVLVRCFKTISYSRLDTEHQQLVREKLGGIAPFPDLRCFTLVASTGAEP
                     DWNRVDRSHRYRVIPMVSADFVKQFPMFSQLLHQFGVSTEFDVRPDGEWLVDLEEKTY
                     NVFYVPDAVGSPYVPVQDGFVGKYGVKSVLCFGGMLPSRDLFAVILFSKTPVPRETAA
                     LCKSLALSLKTSLLAFDEASCTDSGASQQSGSAPEPPASLRHQSRLAVAEQLLRVYED
                     AVRTQSAGIAQAKQALHDRATALQRSEQALHEQTRIVNSVLRSMGDGVVVTDAAGKLV
                     LANPALESIVGFSPGDVPPIEWVDRYEFFHGDTATPFQRDEWPLARALKGEKVDWLEV
                     YMRASQGVPGRWVSINARPIRDDAGLLCGTVVVFHDITWLKRAQDALFESEYRFRSLV
                     EGARDIIFTLSADTTMTSLNQAFETVTNWSRSQWIGEPLATLLHPDDAGFCQDVLQAI
                     LERGTPLTCSMQISTKQGGYVTGEFVGTPQMRQGRVVGVLGIIRDVTERRRIEDALRV
                     SEERLRSIVQSTKDAIVLVNALMKVAFWNKGAEATFGYSAEEIIGQPVTMIIPEQYHE
                     ELERNVQRVRVLERVQSTSKTLELVGRRKQGDEFPLELSVTSWKGKSDLFFTIIMRDI
                     SERRTAEEQLDRLHYHNQVVLNSAGEGIYGVDREGRLTFVNPAAAKMLGWEADAMIGR
                     SLSALVFSPDLAERPFGALSCPIVETIRDGEVREQVDSAFWRKDGTSFPVEYVSTPIS
                     ERGELVGAVVVFKDTTDRKRAEAQLQDSLRRLRKLSGRMEGIREEERGRIARELHDEL
                     GVGLTCLKIDLSRLGGLLGERLVSGDRAKVDEKIRGMKEQVDSTITSVQRIVAELRPG
                     VLDDLGLVAAIEWQCRDFQRRTGITCHCVVSHEDLRVDPEQATAVFRICQEALTNVTR
                     HAQATDVHVRLEEQNVGLLLQVRDNGRGIPQDRLADARSFGLLGMRERAGLLGGDVQI
                     ETLEGNGTTITLQLPREGKG"
     gene            complement(15160..15582)
                     /locus_tag="PLS35_08300"
     CDS             complement(15160..15582)
                     /locus_tag="PLS35_08300"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNI18700.1"
                     /translation="MAEGGLVRSAGGVVLRQQEVLLIRVSDIKGRPVWSFPKGRLDAG
                     ETPAQAAVREVREETGWSCRIDADLSTTEYWFQREGRRFRKTVVWFKMVPLELTGVPD
                     GEVEEVQWVERTEALRRLTYASDAALLSHALELDPSSR"
     gene            complement(15630..>16466)
                     /locus_tag="PLS35_08305"
     CDS             complement(15630..>16466)
                     /locus_tag="PLS35_08305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI18701.1"
                     /translation="TGASTGIGAACARYLDGLGFTVWAGVRRKEDGEALARSTSARLR
                     VLLLDVTDSDSITEASRTLTDALPEAGLAGLVNNAGISVAGPLELLPIAEVRTQFEVN
                     VIGALAVTQALLPLLRRARGRIVNISSIAGLAATPFLGAYCSSKFALEAMSDALRLEL
                     APWGIAVALVEPGAIQSQIWQRATMSATRTLGGVAPESLALYAQSLARMQDVMAGAAA
                     RAIPAEAVAQVVARALTVPSPRARYLVGKDARFRAVLKWLLPDSAQDRLLTWFLGLSR
                     HA"
     assembly_gap    16467..16566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16654..17292
                     /locus_tag="PLS35_08310"
     CDS             16654..17292
                     /locus_tag="PLS35_08310"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18702.1"
                     /translation="MMAILVLSLLGYFALLPSTAQASRTLVVPISVATYTDTQVGFPT
                     FVMKWDEGAQPDPLALRWGRSQVPVRGTGMTSIQHAFQFAVTRLDASIRPTGTLSLYA
                     TFTGPLSEEGSTAEAALAIGFIALLKGDQLRRDITITGTLEGDGRIGQVDQVAEKTTA
                     AARAGYHVLLVPRGQFYAPRVNRVSLTPESNVLVREVETIEEAYEIMTGKKL"
     gene            complement(17370..>19414)
                     /locus_tag="PLS35_08315"
     CDS             complement(17370..>19414)
                     /locus_tag="PLS35_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNI18703.1"
                     /translation="LTATILAQLGNTFHVLGKDEPATEHLTKALTLAREEKKPVLVAG
                     ILNDLGNALTARRQFAEAIDVYAESRVLANETKQQALAATAQINGAMALLQNQQLGEA
                     HRHLDQAWSDVRSLGDGHAKTAGLLNIGLGYQELFAASTGKSGTAAKRDAGKGRATEA
                     VASTGSAAGLLRQSSEAFTAAGEVAGRTGDARGQSYAWGYLGGLREQEHRNPEALEYT
                     RKATFAAQKVNAPESLYRWQWQTARLLRAEGKEDESLAAYQRAVTLLKPIHYEYSVGY
                     QGRHHSYYESVAPLFVEYEDVLLRRAAAAKTPEQNDQLLVKVKETIEVSRAAELQDYF
                     QDDCVATVASHRGAGALAPGTAVIYPIAFPDRLELLLETSNGLKQVRVPVAGEKLTKE
                     IRSFRRLIQDSQSQNYLSSAQTLHGWLVAPIQQDLQGAGVHTLVVVADGSLRTIPMGA
                     LHDGRHFLVDSLAVAVTPSLALTDMNGAQRRKGSLLSVGLTESVEGQSAPRYAETEVQ
                     AIRTMYGGKLLMNKQFSTPTLEEEIKDQGLGIVHVASHTVVGTEARDSFVLAHDGKIT
                     MDRLSQLVGLQQYRQQPLDLLTLSSCEIAAEDDRAALGLTGLAVKTGARTALASLWTS
                     DDETTTELVSEFYRQLQDPAISKAVALQRAQQKILSQRGHTHPSYWAAFLLINNWM"
BASE COUNT         4078 a         6049 c         5189 g         3998 t
ORIGIN      
        1 ccgcgccctt gccgtattgt ttttcaatct gagccagggc caggtccaac gcgcgtttct
       61 tttcatcttt ttcagacatt cataactcct tctcgcgaca tgactcgtga agtgtgcctg
      121 tctcgcatgc cgtgggagac aacgcaccag ccggccatga gttgaccacg cgccagactc
      181 agaccaggaa cgcagacaga acagcggatt ataccggact tagaaggaag aagcctagcc
      241 cgcacgggcc tgcgatccat ccaatctcgt caatcaagac gggatggagc cccttcgtga
      301 atctcgcgaa accatgcatc gaaatcccct gatgctccat acctgcttcc ggcatcgacg
      361 tcaaaaaacc cgcagccttc cgtcatagct gccacgtctc cgacgtgaga gggtattggt
      421 cttcgcccgc cgtgcatcgc gagtccttcc tggcgacctg acgcgtaagt atatgaaaat
      481 gcgtttgaga ctgaagggag ctggccaaag agcggacggc taaagtcata gcgcggacgc
      541 gccgacattc ttggtgaggg acaatgcgct tggcaccaag cttgctccta cctggagtat
      601 tcgttcacct cttacggatg caggaggccc ccatgccaca gcaacacatg gacgatcatt
      661 caggacaatc tgcggttcaa ttgcatgtgc ccgccgctcg cgcgccacgc gacttgacct
      721 atgacgaaaa aaaagccgcc gaggcggcct ttcgtggcga accgttcaat cccgcctggt
      781 ctgccgcggc agcagtcgtg tatgaaggga tcgtctcagc gatgtgtcgc atgcaggtcg
      841 cctcactgac tgatatggag gccgtttctg tcgctgagga atgtctcacg cgataaatga
      901 ctcgcgcacg aggtcatgcg gccagacgtg tcgctcaggc tgccgaagct gccagcggac
      961 attcccacag cttcgaatag accggcccgg cgggatgcaa ttcgcttctc atcatgacga
     1021 gcgccttgat cggcaggaca ccgatcgtca ccggttgatc caacgctccg ctttgagcca
     1081 ggacctgtcc aacctgtcgt tccccttctt ttatacgtgc tagcgtcaga tgcggactga
     1141 acggccgtac ctccgcagcc aaaccggcct cccgagatac ctcctccacc gcccgatata
     1201 gtacctgcaa gcgctgcccg tggcttccct gctcccaact ttccagcggt ccagcccaca
     1261 gaactctcgg ctgttggatt cgaggaaacg ttccgagccg ggaaaagggc acttcaatca
     1321 tgtgatgatt ggcgactact tcggaaacca ctgcatgaag ggattcgatc acctcttgcg
     1381 gcgtctcgcc cagaaacctc actgtcagat gcatggaggc tggttgtacc caactgacac
     1441 gcgcctgccg tccgaccgct ggctccagcc gtcgcttgag atcctgctgg ataaccgata
     1501 gggccgcgcg aaaatccgga ggcaattcga cggccagaaa cgccctgatc atccgccacc
     1561 tttcgtgatc aaccagcgac ggagaacatc cagcgcggcc tgcgaggagc gctgcttgat
     1621 gacgctccgg tcgccgccat gaaaacggaa ttccttcgtc aggttctcgc ccggtccccc
     1681 gtcgagcccc acatagacaa gtcccaccgg cttgctcttc gtggcgcctc ccggcccggc
     1741 aatgcccgtg acactcagcc caactgagac gccgcttcgt tcacgaatgc ctcgcgccat
     1801 ggcggccgcc acttcggcac tgaccgcgcc atgccggtcg aggagatcct gagagacgcc
     1861 caacaattcc accttcgcgc gattgctgta actcacaacg ccccgatcca cgtaggctga
     1921 cgatccgggt atctgagtca gccgatggcc gatcaatccg ccggtgcacg attcggccaa
     1981 ggccagtgtc aggtgttgat cggtgagtaa tcgcccgacc accgtttcca tcgtctcgtc
     2041 cgcctcccca tacataatgt ctccgagccg ggtcttcgcg tcttcaaaca ggcggtcgat
     2101 cgtcacaggc cccgactcct tctccggccc tgtgagcgac accatcactt ccatggggga
     2161 agcataaata ccgagctgaa tcgccgtccc gtccggaagc aatccctcca atcgctgatc
     2221 cacaatggat tccggcacgc cggaggtgtg cagcacccgc cgaatgacgg gagtggcagg
     2281 gattcccttg tgcttaccga tccaggcctg caggcgaggc aggactccgt cttggatcat
     2341 cggctccatc tcacgtggca ctccgggtaa ggctgcaaag aacgtgtctt tccagatcaa
     2401 ggcgaacccc ggcgccgtac caaccggatt ggtcagcact tccccgcctg caggaatcat
     2461 cgcctgtcgc aactgcgcct tcgtcggcgt tcgcccccac tcggccaaac gagcgatcat
     2521 cccatcaagt gctgtctttc gtcggctcag gcgttgtccc gtcgcctgcg caaccgcctc
     2581 acgcgtgagg tcgtcgatcg tcggtcccaa tccacccgtg atgatcacca cgcggacgcg
     2641 ccgggccgcc gttttgagca cccccacgat atcggacaac tcatcgccga caatcgactt
     2701 gtatcgaagc tccacaccac acgtcgccag atgatcagcg ataaacaggg agtttgtatc
     2761 cattctgcca ccgagcaata actcagaccc gatggcgatg gtttcagccg tccaggcttt
     2821 ggacttgttc atgcggaatc aacctttata gtttgaaagg cgtgaaggaa tgttcacgtt
     2881 attcgatatg ggcaaagtcc aattcgaagg ttacctgccc gccgctggat ggaaacacgc
     2941 tcaaggttgt cttcgcatgc ggatcgagat cggcgtaggg aaattgggca atcaccttcg
     3001 cgcgataggc aggctgttgg ggccagacgg atgtccgttc gccccgtgca tcaaatcgca
     3061 ccgaggaggg tttgaccgtc cgccctgcct gctccaacac aatgtaactg tcggcggcga
     3121 agttcacgcg gtcgccgaac agcacgacat tcaccagaag gttgccgttc gccatcacct
     3181 gcgcaacgtc ctgatcggtg ggagacagcg cccgcaacga caagtggttg gccatgacca
     3241 gcagactgcc cagcttggtc atgatgaatc cgtgcggagt cagatcctct ttggcgccaa
     3301 accattcatg cagctgatcc ggagacgcac ccttggcagc ggccgttttc ccccgttcca
     3361 tcgtggcttg aatttgttcg ggagtcggct gcacttcgat ggcgagcgac agagccgggg
     3421 ggcccgtccc tgcgacgacg aacaggtaga gcgcgagttt gaaaccggca caccgtcgcc
     3481 gaatggtacg catgatgagg aggcagtacc agatcgatag gggcctgtca attcgctgcc
     3541 gccgcacccc gccatcggtt gacagcctag gagacgaaat gctaaagagc taattcttat
     3601 tctttcacat tatcaagaag ttgcctggag ggttcctcat gtccagcccc gaacaagcgc
     3661 cccgccgtca gtacgtgaat ttcaccttct acaaagttga tccggcatgg cgtcggcttc
     3721 ctgaagatga gcgcacgcgc ggaaaacagg agttcctgcg tgccgtggag gaataccagg
     3781 gaaaagtgct cgtgatcgcg tacacgagcg tcggtattcg cggcgactgc gacctcatgc
     3841 tttggcgtat cagctatgaa ctcgaattgt tccaggaaat gagcaccaag atcctggcgt
     3901 ccggattggg gaagtacatt ctcaatccgt actcgtacct cgcaatcacc aaacgctcca
     3961 tttatgtcga caatcacacc cacgagaatc aggaaagcaa acggctgacg gttgtgccgg
     4021 gcaaggcgaa gtacatcttc gtgtatccgt tcgtgaagac tcgcgaatgg ttcctgctca
     4081 ccaaggccgc gcgacagggc atgatggacg aacacattga agtcggccat cgcttcccct
     4141 cggtcaaact gaataccacg tactccttcg gtctcgacga ccaggaatgg gttgtggcgt
     4201 ttgagagcga caagccggaa gatttcctgg atctcgtcat ggcgctccgg gaaacggaag
     4261 gcagccggta taccctacgc gacacaccga tcttcacctg catccgcaag agcctgaagg
     4321 aagccttaga cacgctcggc ggttaaaatc gattgacact ccctgacggt tcgcacggcc
     4381 ttcgatccag ttgatcgagg gccgtgtacg ttctacccca cttatccatt aagccactgc
     4441 acctccgcag atccatgtca gttgaagctc aaactcaccc ggcctccgat taccgcgcag
     4501 ccgtgtactc agccattctc cccggactcg gtcacctgct ccgcggacgg cataaagccg
     4561 cgatgttttt tggcttcatc acgattctcc tcatcatcct tagtctgggc ctgggacgcg
     4621 tatcgggtcg agcggcggaa gtcttcttct tcatgctcct cgcccttccc tggtgggcac
     4681 tgcagagtta cgatgccgca ctcggaccgg ccgcctcggg ctcggacctc gtgcgaaccg
     4741 gccgcctggc atgggcagag gggcatgata tccggttcct tggcctcctc tttctggtca
     4801 gcgccgccaa cgatgcgatt atcatcgcca agaaccccga ctatctcttg ccatttttct
     4861 gtacaaagtt ggacggagct gccggcttcg tcaccaaggc cctttcccca tttctccata
     4921 cctgggtggg gtacggcttt ctgcgcatca agaagtggtc actcctggtg tatttggtct
     4981 atgcggccta cggaaccacc aatgccctcg tcaatttaac ctgtttcgga ccgggtcgaa
     5041 ttcggaacac tctgctgatc gccctcattg cctttaccag ctacatcctc tggcgacgac
     5101 ggatgttcca gcgttgacca gatccgctcg gcagttgaag atcgcatttt gacactcggc
     5161 tctaggctgt gttacctgta atagcgatac cgttccccat cctcagatgg agcagctcct
     5221 atggccgaaa acaatgcggt ctatgcgatt cgccaccctg acggttctgt gaccctgtat
     5281 atcgacgaag aatatgccat cgatcggggc gtcgaccccg ccaagctggt gcgggtcgaa
     5341 atcccgcgcg aattgttcgt cagcggcagc atccaacaca ttcgggaata cgtcgcggtc
     5401 taccttgaaa atagccatca gggcacggcc tgagcgaacc ttttacctcg cagagaggcc
     5461 acaccatgtc cgcgttactc acctcggaac ttatcgcctc caccatcgaa caagcaccga
     5521 ttcagggcag ggtcatgctc aagctgctgc tcttgcagca ttttgacatc acgcccgagg
     5581 aaatcagcta tatcgccgcc gaccgaccgg atcctcgctg cgtggccggc tccaaaccga
     5641 tccaccagat ggtcaagcag gatgcgttgc gggatgtgac caatcgcaga gatgagtatc
     5701 gccgccgagt tcgtctgcgt cgggagcgtc tctggctgca gatggaagcc atgaagggca
     5761 tgatcgccct ggctgaaggc atgattcggg cagcgaccga tctgctgcgc acgcgtttca
     5821 agctcgacgc tgaagccgtg aacgccatta cgcaaagcgc ccgcacggca gtccctaagc
     5881 cggcgattcg atcgttgaac cgcaggtggg acgaggatga gatctctgct gagtcctatc
     5941 aggaggccag gcttggtctg gaaattcagg ctcaactccg cttgattgag aagtacagga
     6001 agcgcctgga agtctcggag cgggaatgga aatcgatcaa cgccgctcca ctgcaggatc
     6061 acgagattgg ccatatctgg gggattccgg cagggagcct ggctggccgc aaggtgaaac
     6121 atctccacca gtatcttcac gccattcaat ccgccttgca aaaggcgtca gcccagacag
     6181 tcccgacgac gggagcactt gatctctgga aagaaacgct tgccgtctta ggcgaacggc
     6241 cggttgaacg atctatcgcg ctgtatgatg gcctagaacg aactgaggaa gccctggtgg
     6301 aaaaactccg gaccttcgcc tggggcaccc tgggggagga cctggaggca aagttctggt
     6361 tgtcactcgt tcatggtgcc agctccaacg ccgtgcactc tgaaaacacg cgctccgtct
     6421 tcggcttgca acggctcctg gcagtcctgt cggagattga ttccagcccg gacgcccttg
     6481 aagaagaact cctggctcgt acagtggcga ccccgaagga tgtccctgca ctgaccgacg
     6541 aagcagcaca gccaaaggcg gaagagatgg ggcaaatgca gcagcacgtg ctgaccagtt
     6601 tcatcgggga gttacacaac gaccttcaac gctaacgcac tgtcgacagc tgcatggtcg
     6661 attctggaac atcggctggt ttcccaccga cccggcaaga tttctttttc aaccggcttc
     6721 tcagcgggga gagtattttg tataatccac ctatcatgca gcacgatcat gtcatgattc
     6781 ctgtccggtc actggcgaga gtcgcgggtc tgatgttgtg catgctggtc ttgcccctct
     6841 cgatcatgcc ggtggcttcg ctcttcgctg cgggtctcgt tgaagtcacg gaactgttgg
     6901 ctcatcccga tcattacaat catcaggtcg taaccgtcag tggacgcgta agtagttttc
     6961 agcttgccac gaatcgcgac ggtcaaccgg cattcggatt tcttttgcag gacaccgcag
     7021 gaacggtgaa agtggtgggt cttggaaagg cggatgttcg agaaggtgac tacgtcgtgg
     7081 tcgaaggaat cttcagccga ttgcgccaag ccggtcgcgc catcgtctac aacgaaatca
     7141 aagccacctc ggttcaatcc atggcaaaga tgaacccgga cctggtcggc taactttccc
     7201 ccttctttgc cggtgctatc tcctctacaa cccggttgcc ttctctctcg atatcgatca
     7261 ctctgacatc gaagaccaag gtcttccccg caagcggatg gttgaggtcc aggacaatga
     7321 cctccggctt gatctcggcc acatagggaa acacaaccct cccgtcagga gccgtcgttt
     7381 ccagcttcgc cccaacgcgc tgagcctcca ctggcacacg atcccttcgg acttcgaata
     7441 gcccctccgg atgtctctcg ccataaccat ccacgggcat aacagtcacg cgttttgcag
     7501 caccgattgc catgccctcg agtcctgctt cgagcccgcg caagatctca tgctgtccgt
     7561 gggtgtagac caacggagcc tcaccgacgg ttgtgtcgat gacctgctca tcttcgaggc
     7621 gaatgacgta ttcgactgag acgacgtctc ctgccgtgac gtgcatgtgc cagttccttt
     7681 ttccacagtc tttgcgacgg tgtgatcaat tgcccagact cgtgcgaaac attgtgctca
     7741 ggttcgtctg caacggcgat ggtcgtcgct aagatggttc cgacttcgat cagcctatca
     7801 cggcaatcga ccatggtcat gcgaattccg gaagctcaga ggagcttcgc gaaactctgt
     7861 ccggattact caagaaggat cgaaggagga atggagggac tgcctaccaa tgccggaagg
     7921 gtcccgaatc gaggtgcaca aactttccgc gcggatagta cccgactccg ccacagccaa
     7981 gtcgcaacgc gacttcccga acagtccgca aaggcacgcc gggaatctgg acatcaaccg
     8041 cctgtcccga cacatgataa ctatggcgtg ccgcgcgagt gcccattctg atgagctgtt
     8101 cgttatattc aggcgaacgg taggcggata cgatatgaat gtcccggcgg ccaccgacct
     8161 tctgttgaac gagtgtaacg aattcgatga gttgcacatc catcgtcgtc gtctcattcg
     8221 aatggtgaca ccgcaaaaaa taattgaggt cgtcgagcgc agactgatcg tatgttccgc
     8281 tctctgtccg gaacgtgact gacaagcgtt cttcggtctg cagattgaac aagctgatgc
     8341 gtcctgtcgg gagtcgcgac gctcgcgctt ttgcaggaag agccaggttg gcaacgaata
     8401 gcgccgtgcc tacggtgacc gtacgcaaaa aattccgtcg actcgacaca gaaagaacct
     8461 cggatggcat caattcgtct cctcgcgaaa cgtgcgagtc atctgtcgtg ggcactctcg
     8521 catcacgccg caatgtctgt tgacagagac ggttcgcaca cactggcgga cgcaaactgc
     8581 acggcaacac aggcgctatg caaataggct gacgatacct cgggaggaac caggccagca
     8641 gagaccggaa gactcttctt cttaacaaat accaaagaag tggtcaacca atttacactc
     8701 gcgagcggcg tatccctgct gccgcagcgt cgctcgcctc gttcgcatga caccataccg
     8761 cagcagacac gtccgagaga tcaatgctcc gtcatcgaag cgcgcggcga gagaccggga
     8821 aaccggcggg cctcaagaga tcgttccatt ccatattggc gaagtcgccg gtacaacgtg
     8881 gagcggctaa tgccgagctc ctgggcggcc cggctcaagt tcccctgtga gagggtgaac
     8941 gctttcatga tcaggtccgt ttcgatccgc tgctgattga ccttcagtga gattgatccg
     9001 tcgtcctgcg gcggggcctg gcgaggcaaa tccaaatcgg cagcggtgat atgcgtgcct
     9061 tccgccatga ccacggcacg tccgatcctg ttggataact cccgcacatt gcccggccag
     9121 gaataggcct gcatcgcctc cagcgcctca cgagtaaacc cgtacagagg tttattgtag
     9181 tgagcagcgg cctgccgcag aaacgccatg gcgatcagcg acacatcctc gcctcgttcc
     9241 cgtaacggag gcaggttgat gtgcaccacg cccagccgat aatacaggtc atcccgaaag
     9301 gtccccttct cgatcgcttc cttcaaattc acgtttgtgg cggcaatgat ccgcacgttc
     9361 atctcaatcg cctgatgccc gccgactcgc tcaaacgtgt gatcctggag gaaccgcagt
     9421 aacttgacct gcaacgcatt gggaagttca cccacctcat cgaggaacaa cgtcccaccc
     9481 tgggcaaact ccacttttcc ctttttctgc ccgacggcgc cagtaaaggc gcctcgctca
     9541 tagccgaaga gctcggattc caggagactt tccgggatgg ccccgcagtt gatcggaata
     9601 aatggcccct gtttgcgtaa tccccgggcg tgaatcgcac gcgccgtcag ttctttgccg
     9661 gtgccgcttt cgccagtgat cagaatcggc gcatcactcg tcgacacctt ccgaatagca
     9721 tcaaaaatcc gattgatact cgcactcatg cccaacatgc cgctgaattc ttcgggcccg
     9781 ccaaccacca tggccccgcg cgcttcctgt tccagatcgg cgacccgcgc gactcgttct
     9841 atactctgca gtaattgcac tggatcaagg gacttggcga ggacatcaca ggccccgcac
     9901 tgaaccgcgc gcaccgccac acttcgctca ggtctcccgg tgtacgcgat gactttcccg
     9961 gaataccccg tctgacgcaa tttctggagc acattcaacc cttcttcggc agacggctgc
    10021 gatggcgaat cgatatcgag cagcatgacc ggcgaagcct gttcatgaat gggcaacagc
    10081 gtgtccgtcg tccacattgc cgtcgagact tcgaactcgt cctgaatcaa tgcctgcgct
    10141 gcggacacgg tcgactgatc aggtccgaca attactacac tatgcttcgc cacgattcgg
    10201 ctcctttcgg ctggtaagac cacgggcgcc attcaggcaa accatcgtct cacgaacggc
    10261 aagatcgaga taaggatgcg gctcaagtcc gcatgacagc tcgttatgag accgtcgacg
    10321 gagggtcgag agagtcgcac ataaacacat ctccgccgaa tgctttcgcc agcgctggca
    10381 aggtgccatt cgcgtcctga gcggtgcgat caagcatcgc gatgattggc acttgcgggc
    10441 aggtacgttt caaacgggga accgctgtct gcagcggcgt ggagcatgat gacaacccaa
    10501 cgacaatcac atcgggacgc agttcctgaa ggtgatgttc caaatgaggc acagcggacg
    10561 tcatggtggc ttggtatcca tggagcgcaa accagtcggc atagcgatac ccgctggtga
    10621 catcttcata aatcaccgcg accgtcggcc gttttccgga acgcgcccca ttgccgcctg
    10681 ttgatgattg ccccataaga ttccctcccc agatgaatgg catcaggttg tggtgtgcat
    10741 ctttgcgagt tggaccaacc ggtgtttcat ttgcggcccc caccaggtca ggtcctgtaa
    10801 catcgcggcc tctcgtgggc tcagtcttgt caggagatcc gaccaccctt gacgaaacga
    10861 ccccgttcgt cgatcaacag atgatcgctc ggcagccgca tggccttgcg ctgcgtcaaa
    10921 aatggccact aatgtcgcgg tattagagat ccaggccctc tgcatttgga agagcatagg
    10981 ccgggagggt cgaggaggct atcaacagag gcgctattcg tgtgtcacaa taaaacagtg
    11041 ccgattcgtc atcgttctat cgacacattt aggcacatca gcgccgtcag acaacgaggc
    11101 catgctggag ggcataccgg gtcagctcgg cattgttctt caagttcatt ttgtccagaa
    11161 ttctcgcccg atatgtgctg atcgtcgtca cgctcaggtg gatactctcc gcgatatcgc
    11221 tgacggtttg tcctgaggcg atgaggcaca gcacttcata ctcccgatcc gacaatcgct
    11281 cgtgcggtaa cttgtcgact ccggtgcgca ccgttaacgc cagcgcttcc cccaccgaag
    11341 cactgacgta ctggccgccc gccatgactt tcttcgccgc atggaccagc tcttccggcg
    11401 cactcgcctt cgtgagatat cccgccgcgc cggctttgaa catccgaatg gcatactgat
    11461 cttcgggatg catgctcaag atcaagaccg gcaacgtggg cgccagctgc ttgagttcct
    11521 tgaggacctc cgggccactg cgtccgggca tgctgatatc catcacaacg agatcgtagg
    11581 tctgttcacg gacctggtcc agcatctcct gcgcgtttcc acattcggcc atgctagcgc
    11641 cttcgaatcc ttcctccaga atgtccttca ctccacgccg gaaggaggga tgatcatcga
    11701 cgaccagaat acgtacggtt gctgtgtttc gcatcgttcc ctccatcatg cgctatccct
    11761 ttccttctcg aggcagttgc aatgtgatag tcgtcccgtt gccttccaag gtctctattt
    11821 gcacatctcc accaagcagc ccggctcgct cccgcattcc gagcagaccg aatgatcggg
    11881 catcggcaag tcgatcttga ggaattcctc gcccgttgtc gcggacctgc agcaataacc
    11941 ccacgttctg ctcttccaat cgcacatgca cgtctgtcgc ctgtgcatgg cgggtgacat
    12001 tggtcagcgc ctcctgacaa atgcgaaaga ccgcagtggc ctgctccggg tccacacgca
    12061 aatcctcatg gctgaccacg caatgacaag taatgccggt acgacgttga aaatcccggc
    12121 attgccattc aatcgccgcg accagcccca gatcgtccaa cacgccgggg cgcaattccg
    12181 cgacgatccg ctgcacagag gtaatggtgc tgtcgacttg ctccttcatg ccgcgaatct
    12241 tctcatcgac cttggcccga tcgccagaaa cgagccgctc gcccaacaga ccgcccaggc
    12301 gggataaatc gatcttcagg cacgtcaggc cgacgcccag ctcgtcgtgc aattcccgcg
    12361 cgatgcgtcc gcgctcctcc tcgcgtatcc cctccattcg gcccgagagt tttcgcagcc
    12421 gacgaagaga atcctgcaac tgcgcttcgg cgcgctttcg atccgtcgtg tccttgaaga
    12481 cgacgaccgc gccgacaagt tccccgcgct cagaaatggg cgtactcaca tattccaccg
    12541 gaaaactcgt cccgtccttg cgccagaatg cactgtccac ctgctcgcgc acctcgccgt
    12601 cccgaatcgt ctccacaatg gggcaactca gcgcgccgaa cggtcgttca gcgagatcgg
    12661 gagagaacac caaggcggat aacgaccggc cgatcatggc gtctgcttcc caccccaaca
    12721 tcttggccgc cgccgggttg acgaacgtca aacgcccctc gcgatccacc ccgtagatgc
    12781 cttctcccgc cgaattcaga acgacctgat tgtgataatg caggcgatcc agctgctcct
    12841 ctgccgtccg ccgttcgctg atatcgcgca taatgatcgt gaaaaacaga tcggattttc
    12901 ccttccatga cgtcacgctc aactccaacg gaaactcgtc cccttgcttc cggcggccca
    12961 cgagttccag cgtcttgctc gttgattgca cacgctccag cacacgcacc cgctggacgt
    13021 tgcgttccag ctcttcatgg tactgttccg ggatgatcat cgtaacaggc tgtccgataa
    13081 tttcctctgc cgagtatccg aaggtcgcct ccgctccctt gttccagaag gccaccttca
    13141 tgagggcgtt gacgaggaca atggcgtctt tggtcgattg cacgatcgat cgcagtcgtt
    13201 cttcgctgac ccgaagcgca tcttcgatcc gccggcgttc ggtcacatcg cgaatgattc
    13261 ccaatacacc caccacacgc ccctgccgca tttgcggggt gccgacgaat tctccggtta
    13321 catagccacc ttgcttcgtg ctgatttgca tcgaacaggt gagcggcgtg ccgcgctcga
    13381 gaatcgcctg caacacgtcc tgacaaaatc cggcatcgtc tggatggagt aaggtggcga
    13441 gcggttcgcc gatccattgc gaacgggacc agttggtcac cgtctcaaat gcctggttga
    13501 gggaggtcat ggtcgtatcg gccgagagcg tgaaaataat atcccgcgcg ccctccacca
    13561 gactgcggaa tcgatattcc gactcaaaca aggcatcctg cgcccgcttg agccaggtga
    13621 tgtcatgaaa caccacgacg gtcccgcaca gcagtccagc gtcatcccgg atgggtctgg
    13681 cattgatact cacccatcgc cccggcactc cctgcgaggc ccgcatgtag acttcgagcc
    13741 aatcgacctt ttcccctttc aatgcgcgcg ccagcggcca ttcgtcgcgc tggaatggcg
    13801 tcgccgtgtc gccatgaaaa aattcatagc ggtccaccca ctcgatcggg ggaacgtctc
    13861 cgggagaaaa accgacgatg ctctcgaggg cgggattggc caagaccagt ttccccgctg
    13921 catcggtgac gacgacgcca tctcccatgc ttcgcaatac cgaattgacg attctggtct
    13981 gctcatggag agcctgctca gaccgttgca gcgccgtagc cctgtcgtgg agcgcctgtt
    14041 tcgcctgtgc aatgccagcc gattgtgtcc tgaccgcatc ttcatagacc cgcagcaatt
    14101 gttccgcgac cgccaaacgc gactgatgac ggagacttgc cggaggctca ggcgctgatc
    14161 ctgattgctg gctcgcacca ctgtctgtgc aagacgcttc atcaaacgcg agcaaggatg
    14221 tttttaaact gagggcgagt gatttgcaca acgccgcggt ctcacgcggc accggcgtct
    14281 tggagaacaa aatcaccgcg aataaatcgc gcgacggtaa catccccccg aaacacaata
    14341 ccgacttcac accatatttc ccgacaaatc cgtcctggac aggcacatag ggacttccca
    14401 ccgcgtcggg cacataaaac acgttatacg ttttctcttc caaatcgacc agccattcac
    14461 catccggcct cacgtcgaac tccgtggaca caccgaactg atgcaggagt tgcgagaaca
    14521 tcggaaattg tttcacgaaa tcggcgctga ccatcggtat gacacgatat cggtgagaac
    14581 gatcgactcg attccaatcc ggctccgcgc cggttgaggc cacgagtgtg aaacagcgta
    14641 aatcggggaa cggcgcgatg ccgccgagct tttctctgac cagctgttga tgttcggtat
    14701 ccagccggct gtaggaaatg gttttgaagc accggacaag gacacaatcg cgttgccccg
    14761 tggtcgggtc gccgagcgtg tcgaaaagat agttcaccgc cttcgaggcc gcctcctgca
    14821 aagaccgcga ctgttcgccc agctggcgca ggacggccga gcattccgtc atttccaaca
    14881 gggaaaagcg agccagacta tacacaggcg ggccctcggg tgaacgatgg gggatgactg
    14941 atcaacgcgc cactgattga cgggtgccgg gagatgccga atcgtgaggg aaggcgcgtg
    15001 tcgggaactc tggccttcca aagcgaggcg cagaaccact ctgaaggcga gtctagttaa
    15061 gggcaccagg cttgtcaagg cagggaggac ggatatcatc agacgcgtgg tctgagaaaa
    15121 caacagatct gttcagatga agactgggag aaattgtcgc tatcgagagg aaggatccaa
    15181 ctcaagcgca tgcgacaata acgccgcatc ggacgcatag gtcagtcgcc gcagggcttc
    15241 cgtccgctca acccactgca cttcttccac ttctccgtcc ggaacgccgg tcagctcgag
    15301 cggaaccatc ttgaaccaga ccaccgtctt gcggaatcga cgtccctctc gttgaaacca
    15361 atattcggtt gttgagagat ccgcatcgat gcggcaagac cagcccgtct cctcacggac
    15421 ttcccgcacg gcggcctgcg ccggcgtttc accggcatcc aggcggccct tggggaagga
    15481 ccagaccgga cgacctttaa tgtcggacac ccggatcagc aacacttcct gctgccgcaa
    15541 gacgaccccg cctgccgagc ggaccagtcc tccttcagcc atgccgctgc tctcttttct
    15601 gacgcccgct tggtccgcag tgcgcaacat tacgcatgac gcgacaatcc taaaaaccat
    15661 gtcagaagac gatcctgagc gctatcgggg agcagccact tcaggaccgc tctgaagcgc
    15721 gcatccttgc cgaccagata acgagcacgc ggcgatggca cggtcaacgc acgggccacc
    15781 acctgggcaa cggcctcggc aggaatcgca cgggcggcag ccccggccat cacatcctgc
    15841 atacgcgcta aggactgagc atacagggcc aacgattctg gcgcgactcc gccgagtgtg
    15901 cgggtggcgg acattgtggc ccgctgccat atctgagatt gaatggcgcc aggttcgacc
    15961 agcgccactg cgatgcccca gggtgccagc tccagccgca aggcatcact catggcctcg
    16021 agcgcaaatt tggagctgca ataggcaccg agaaacgggg tggcagccag cccggcgatc
    16081 gaactgatgt tgacgatgcg ccctcgtgcc cggcgcagca acgggagcaa ggcttgtgtc
    16141 acggccagcg ctcctatgac attgacttcg aactgtgttc ggacctcggc gataggcaag
    16201 agttcaagcg ggcccgccac ggagataccg gcgttattca cgaggccggc cagccccgct
    16261 tccggcaacg cgtctgtcaa tgttcgactt gcctcagtga tcgaatcgga atcggtcaca
    16321 tcgagcagca gcacacgcag acgagcggag gtggaacgag ccagggcctc gccatcctcc
    16381 ttacggcgca cgccggccca caccgtgaaa cccaatccgt ccagataacg cgcacaggct
    16441 gcaccgatgc cagtcgatgc accggtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnaggc gaatttgaca atcgggcatc ctcatctaga cttgattcat gcgcgtacac
    16621 actttctttt ccagaagcgg cacagcgtca agaatgatgg cgatcttggt cctctcgctg
    16681 ctcgggtatt tcgctctgct tccctccacc gcacaggcca gtcgaactct cgtcgtcccc
    16741 atttctgtcg caacctatac cgacacccag gtcggcttcc cgacgttcgt gatgaaatgg
    16801 gacgaggggg ctcagccgga tcccttagca ttgcgctggg gccgtagcca agttcctgtt
    16861 cgcggaaccg gcatgacgtc gattcagcac gcgttccagt ttgctgtcac acgtctggat
    16921 gcctccattc gcccgacagg aaccctctcg ctgtatgcga cattcacggg tccgctcagt
    16981 gaagagggat caacggcgga agccgcgttg gcgattggat tcatcgctct gctcaagggt
    17041 gaccagctga gacgcgacat caccatcacg ggcacgttgg aaggcgatgg tcggatcgga
    17101 caggtagatc aggtcgccga aaaaacaacc gcggcggcca gagccggcta ccacgtgctg
    17161 ctcgtaccac gagggcaatt ttatgcccct cgagtcaatc gagtcagctt gacgccggaa
    17221 tcgaacgtcc tcgtccgaga agtagagacg attgaagaag cctatgagat catgacgggg
    17281 aagaaactct aacgtccccg cctaccccac tccgcaccgt tcacaccctg tccgcttgtt
    17341 acctcgttca aagcttctac ggattggctt cacatccagt tgttgatcaa caggaatgcc
    17401 gcccagtaac tcgggtgcgt atggccgcgc tgcgaaagaa tcttttgctg tgcccgctgc
    17461 aaagccacag cttttgaaat ggcgggatcc tgcaactgcc ggtaaaattc cgataccagt
    17521 tcggtcgtgg tttcatcatc cgacgtccac agactcgcca gcgccgtcct cgctccggtc
    17581 ttcacggcga ggccggtgag cccgagcgcc gcacgatcat cctcggcggc aatttcgcac
    17641 gagctcagtg tcagcagatc cagcggctgt tgacggtatt gctggagtcc caccagctgg
    17701 gacaaccggt ccatcgtgat cttgccatca tgcgccagca caaacgaatc cctcgcctcg
    17761 gttccgacaa ccgtatgaga tgccacatga acgattccca gtccctggtc cttgatctcc
    17821 tcttccaagg tcggcgtaga gaactgtttg ttcatgagca gcttcccgcc gtacatcgtt
    17881 ctgatcgcct gcacttcggt ctccgcgtat cgcggcgcgg actggccctc gaccgattca
    17941 gtcagcccca ccgacagcaa acttcccttg cggcgttgag ctccattcat atcggtcagc
    18001 gccagactcg gcgtgactgc gacagcgagc gaatcgacga gaaaatgccg cccatcgtgg
    18061 agcgctccca tgggaatggt cctgagcgag ccgtcggcca ccaccacgag tgtatggaca
    18121 ccggcgcctt gcaagtcctg ctgaataggt gccacgagcc acccgtgcaa cgtctgcgcg
    18181 gaagacaggt aattctgtga ttgcgaatcc tggatcagcc gcctgaacga acggatttct
    18241 ttcgtcagct tttcccccgc cacaggaact ctgacttgtt tgagtccgtt ggacgtttcc
    18301 aggagcagtt cgagccggtc cggaaaggcg attggataga tcaccgccgt gcctggcgcc
    18361 aatgcccccg cgcctcgatg actcgcgacc gtcgcgacac aatcgtcctg gaaataatcc
    18421 tgcagctcgg ctgcccgcga cacctcaatc gtttccttga ccttcaccag caactggtca
    18481 ttttgctcgg gcgtcttcgc cgccgccgct ctccgcagca agacatcttc atattcgaca
    18541 aacaatggag ccacggattc gtagtacgaa tgatgccgtc cttgatatcc gacggaatat
    18601 tcatagtgaa tcggcttcaa cagcgtcacg gcacgctgat acgcggccag ggattcatct
    18661 tccttgccct ccgcgcgcaa caatctcgcc gtttgccact gccagcggta cagcgattcc
    18721 ggcgcattca ccttttgagc ggcgaacgtg gcttttcgcg tatactccag cgcctcggga
    18781 ttgcgatgct cctgctcccg caatccaccc agataccccc aggcatatga ttgcccgcgc
    18841 gcatcgccgg tgcgtcctgc cacttcaccg gccgctgtaa aggcttccga tgattggcgc
    18901 aacagaccgg ccgcagagcc ggtagaagcc accgcttccg tcgcacggcc cttccccgca
    18961 tcccgcttcg ctgccgtacc ggatttcccc gttgaggcgg caaacaactc ctggtatcca
    19021 agaccgatgt tcaacaggcc cgcggttttt gcgtggccgt ctccaagtga ccggacatcc
    19081 gaccaggctt ggtcaagatg acggtgcgct tcgcccaatt gttgattctg cagcaaggcc
    19141 atggcgccat tgatctgtgc cgtggctgcc agcgcctgct gcttggtttc attggcaagg
    19201 actcggcttt ccgcatagac gtcgatcgct tcggcgaatt gccggcgcgc cgtcaaggca
    19261 ttacccagat cattcaaaat cccggcaacc agcacaggct ttttctcctc ccgtgccagc
    19321 gtcagggctt tggtcaaatg ttcggtcgca ggttcatcct ttccgaggac atgaaaggtg
    19381 ttgccaagct gggcgagaat cgttgcggtg agac
//
LOCUS       DAOQEW010000058        19282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_718_length_19282_cov_7.704582, whole genome shotgun sequence.
ACCESSION   DAOQEW010000058 DAOQEW010000000
VERSION     DAOQEW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_19282_cov_7.704582"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="PLS35_08320"
     CDS             complement(<1..755)
                     /locus_tag="PLS35_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNI18704.1"
                     /translation="MPLKFALYPGCAAKGATPELYQSTMAIVGRLGIEVVELAASSCC
                     GAGVIGEADPDLALALNARTFAQAERMGLDVMTICGTCQGVMSAANRRLKSEPATLER
                     VNKILAQDGISYGGGVQVKHLLWIAVRDLGLSRVAGTVRSSFREFRIAPFYGCYMLRP
                     SWELGFDDPENPSSLEKIIRAVGGEPVAYAGRTKCCGFPIILEKEAIAVAMAGTNMKD
                     AKDHGADFMVTPCPLCHMSLDIYQERAGRAVKT"
     gene            complement(880..1326)
                     /locus_tag="PLS35_08325"
     CDS             complement(880..1326)
                     /locus_tag="PLS35_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18705.1"
                     /translation="MIAERVLVVLCLTAVGCGLAPLAIAAGDQAPPSTTEKMIRDTKE
                     AVESTKQYTIQQKDSFQKTVQAELAEMQTKITELQKKTSAASVEARKDMQKALQDLER
                     KKDEARKQLDEVSHSTSSAWSTLKDNVNAAVADLKKSYKDTVSKLP"
     gene            1518..2387
                     /locus_tag="PLS35_08330"
     CDS             1518..2387
                     /locus_tag="PLS35_08330"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNI18706.1"
                     /translation="MSAELAPMSDPASTSPLEQLLIERIRKGAIELPLLPQVASRILA
                     MVYDPDAEAAKLAALIHQDQALAAHVIRIANSPAYMTRNPVVSLQHAVSMLGMNLMSE
                     LAFSASIKGSAFKVPGWDGEVKSLWHYSLASGAYAKEIARMRRFNVESAYLCGLLHGI
                     GRPVVLQTLVALAKEQGSPLTKELLHQLLDGYYIQVGLLVADEWGLPPPVVESIGFHA
                     DYHYAKTAKQECMTTCLAGRLAKHFLDPEGFDEATVRDHSVFADLNLYPKDIDALLAH
                     KDKVQAVVEAMPL"
     gene            2387..3805
                     /gene="sthA"
                     /locus_tag="PLS35_08335"
     CDS             2387..3805
                     /gene="sthA"
                     /locus_tag="PLS35_08335"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNI18707.1"
                     /translation="MPATAYDIVVVGSGPAGQKAAIQGAKAGKKVVLIEQEPGIGGNC
                     VYRGTIPSKTLRETAVQFERLKRSSEVFEGKMRIDVPMSVLLHRLDEVVKAHECYMAS
                     QLTRNGVTYRHGRARLLSPHEVQLETVDGACQTLTAETIVLATGSRPSSIPEIPVDHE
                     HVLDSDSILSMIYLPRSLTILGGGVIACEYASTFALLGVEVTIIDRAKRPLPFMDHDI
                     VDVFQRSIERQGGRFYSGHTVTEVAWDGVSSVVARLANGMAVKSEKMLVALGRQPNVE
                     ELNLEAAGLSLDEKGHIPVNDHGQTAVPHIFAAGDMLGRPPALASQAMEDGRRAVSHA
                     LGLPVGDSLNQVPIGIYTIPEIASIGLDEEQAAARYRGPIIGRAPFTEIAKGQITGAC
                     DGLLKLVSDPSGERLLGVQIVGENATELIHLGQMALQDGATIDRFIDTIFSFPTFAEA
                     YRVAALDILGQRRKRRDEAQAA"
     gene            3900..5597
                     /locus_tag="PLS35_08340"
     CDS             3900..5597
                     /locus_tag="PLS35_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18708.1"
                     /translation="MNREERRREEKLQGRNAGGPAAALAGLREAQMHHQAGRLDDAER
                     GYQQLLARMPAQPDALHGLGLLLYRRGNLRDALTRLAQARAADARNPVYCFNHGVVLQ
                     RAGLLPDAVDAYNRAIQLNPRYIDPRTNLGNAYKELGRLAEAQATYEQVLALNPDHAE
                     AHNNLGVVLKEQGRLDAAADAYRKAIALKPAHAEAHNNLGLVLLEQGLVDDAIRSFER
                     ALQAHPGYGTALYNLGIAWIWREDIPRALHCFAETAKAKCAHGRPVTDSAVFRSRVKH
                     DAEQIGYLFERGLIGEEWRGYHDALLQLCGRLEPAPGTVSANRVALSPADLQPIAPSF
                     NRLLYIAPCDTIAEGALAPDLDHAAIEARYLATQPEVTYIDGLLSEEALRRLREFCWT
                     STIWKKDYENGYIGAFLGDGFASPLLLQIAEELRRRLPRIFGTHRLTQAWAFKHDSAR
                     RGLNIHADAAAVNVNFWITPDDANLNPESGGLVVWDKEAPREWDFKTYNSDKARGSIY
                     EWLTSQGAKEIKIPYRANRAVVFNSDLFHETDDIAFKEGLTNRRINITLLYGHRHRP"
     gene            complement(5563..7689)
                     /locus_tag="PLS35_08345"
     CDS             complement(5563..7689)
                     /locus_tag="PLS35_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNI18709.1"
                     /translation="MSQTTPKILIADDDPLARLFVKNALEPAGMIVLEATGGKDALTK
                     FEAIGPDLVILDIMMPDMDGYLTCSRIRSLPRGKRVPILVLTGLDDAQSIGQAYQHGA
                     TDFITKPVNATILCHHVRYMLRTNNVLHALIRSESRLELAQRIARIGNWDWNPKTNRF
                     AMSNELCRLVGVRPQDFAGTFEAFLALVHADDRSIVTGALQKLVAQHTPCDIDHRIVL
                     PNGTDFVIHLQAEGVREEETDEITVIGTAQDITERKQAERAIHQLAYYDSLTGLANRV
                     LFKDRLSNAISYASRHQQHLATLFIDLDRFKIINDTLGHTVGDRLLTHVAERLSESVR
                     QSDSVSRHADQEPMHALARLGGDEFTILLTALPQPEDAGRVARRILESLAHPFSIEGH
                     EIFISASIGISIFPSDGSTVEALLKNADTAMYHAKEQGRNNCQYYSSGLNAAAAERLD
                     LENELRRALEREEFVVFYQPKLNIHSRRILGAEALVRWKHPKRGLVPPGVFLNAAIDT
                     GLIRSMDEWVLREACRQVKAWEKAGLPPITISANVSNSLFHGRTLPTTVADALRDSGL
                     NPAQLELELTESIAMRDVEASVTMLEGLRTMGVRLSIDDFGTGYSSLSYLQRFPLSRL
                     KIDQSFVRDLLTNENNVKITRAIIAMAHSLNLSVLAEGVETEGQLAKLREEGCDEVQG
                     YLFSRPVCAEDFEKLLKGDADARTAA"
     gene            complement(7913..8641)
                     /locus_tag="PLS35_08350"
     CDS             complement(7913..8641)
                     /locus_tag="PLS35_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18710.1"
                     /translation="MADASASRLPRTWLPALLVSIIISTLCAAALYVLLAQHDRSLVA
                     QRNASAVMAAEYSARATAQTLAWFSESMGGDNFDSIQQTLEQHAQQANLLAAAVIMDN
                     NVVVASSNPAAIGTTLQDAASLAARRTQSGVITSGIEQGRAAFIVMEPFRRDNRVAGW
                     IRLAVATPPEAAAPRSDDDLGRDVALVIAPLLLLTATLLMLTMRGLMSRVRTLLAGIV
                     LEAREQGPHTIPGGLDTTERGEMA"
     gene            complement(8670..9056)
                     /locus_tag="PLS35_08355"
     CDS             complement(8670..9056)
                     /locus_tag="PLS35_08355"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18711.1"
                     /translation="MSSFTQRRLTMEQRQHERFPAHFRSAFSSVNRVEGDGTVVDLSL
                     RGCGIVSQTAMHPGTTLTLRIHLPDPETMLTVQQAVVRWCRDGRIGLEFQSLPPDEWA
                     RLQRIVKALTRHPYERTQDAQEGSGA"
     gene            complement(9112..10563)
                     /locus_tag="PLS35_08360"
     CDS             complement(9112..10563)
                     /locus_tag="PLS35_08360"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNI18712.1"
                     /translation="MTLNTDMQVNRIDDYLWEIPPSEKAGMLVPARIYASPAILGAMD
                     RGVFDQVTNVACLPGIHRYALCMPDGHWGYGFPIGGVAAFDPEDGIISPGGVGYDINC
                     GMRLIRTDLTRAEVQPKLELLMTELFRRVPAGVGSRGFVNVNRREFREVMRKGAVWCV
                     AKGYGWDEDLERIEERGCLDGADPAHVTDYAIERGINQLGTLGSGNHYLEVQVVSDKN
                     IFDRETAAAMGLTGRDQVVVMVHCGSRGFGHQVASDYLKVFEKAMRRYGISVKDQQLA
                     CAPFRSPEGQEYFGAMNCAANTAFANRQVITHQIREAFAAVFDSTPHALGMHLIYDVA
                     HNIAKVERYGDGEWLVHRKGATRAFGPGSPELPDCYRAIGQPVICGGSMETGSYLLVG
                     TNRAMHDTFGSTMHGSGRTMSRAQAKRSVRGEQLLRDMKQQGILVKAVSMSGLAEEAG
                     LAYKNISDVVNTVDQAGITKKVAELKPIGNIKG"
     gene            complement(10612..11052)
                     /locus_tag="PLS35_08365"
     CDS             complement(10612..11052)
                     /locus_tag="PLS35_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNI18713.1"
                     /translation="MPGTFRFLDDIAMADMAFEAEGDSLASLFDAATQALLQSLADPA
                     TVAQTWQQVVDMEEPDIDTLLFEWLNRLVYLKDAQAVVFHHATLTVQAAPGQSAWHLH
                     AELVGAPVDQATQDLRSDIKGVTKHLYAVTQTGNSWKARVVLDV"
     gene            complement(11058..12032)
                     /locus_tag="PLS35_08370"
     CDS             complement(11058..12032)
                     /locus_tag="PLS35_08370"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNI18714.1"
                     /translation="MRLTFTIQRFNPETDQQPHQEEYRLDIGRGMTVLEALIRIKNEL
                     DGRLALRYSCRSAICGSCAMLINGTQKLACRTSIRKELERHGRISIAPLPNLPLIKDL
                     VVDMAPFWEKIRAVTPWLTPITHPTKRYGSSGQLRLLPDSYQFHNVDACIMCAACVAA
                     CTSHEVSRGFLGPAALAKAARFVADPREPADAKQVRLAALQEADGIWDCTRCNMCVQV
                     CPKDVQPMEAIIRLRRSSLRHGLTTAEGARHITGFVDLVRQEGRLNEALMPLKVLGFN
                     LQRVVDVMPLGVRMFFKGKVPLPFGHTIPGIEQVRAIFAAARRRTTRV"
     gene            complement(12077..13234)
                     /locus_tag="PLS35_08375"
     CDS             complement(12077..13234)
                     /locus_tag="PLS35_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNI18715.1"
                     /translation="MSHSTDQHTNPVPGATVFIAASETDSNLYYATKFIAPDPFIYLE
                     VKGERLMIMSDLEVDRARHQATVDRVVSYSELERRAKAQGVKDPGNIDVIHLVLQDAG
                     VRDILVPPTFPFLHAQRLQELGYRLRTKRDPFYESRVVKSDEEVRHIEAAQRATEKAV
                     AAAHESLRRAEIRDNVLWLDGQVLTSERVKKLINVALMECDCVAQHTIVAGGEQACDP
                     HDEGSGPLPAHRSIIFDVFPRSADSRYFADMSRTVVRGQPSPELKKLYQTVKDAQEEA
                     ITKIRDGADGAVIHQGICDRFEKSGYKTGVVNGRMQGYFHGTGHGVGLDIHEAPRISR
                     TGSLLQEGHVVTVEPGLYYPGLGAVRIEDMVLVTKDGCRNLTNYPKVFELG"
     gene            complement(13286..16699)
                     /locus_tag="PLS35_08380"
     CDS             complement(13286..16699)
                     /locus_tag="PLS35_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNI18716.1"
                     /translation="MRVRPRTVVLSLLGLVVAGILLLFYSRELFGVDILKNFFLQQLE
                     TSLRRKIEVDRIKLVVLPSIRLELTNVGIYGHDDPTHVVFSAKEIDIVLRLLPLLKKQ
                     VVAKRIFLNEPTVTLVRNRSGHWNVLAGMPSAAKDESAYQMFSRLLQIREATIQKGHI
                     TVTDEARPDGVRTTTLETVEMALKVYPGKAEGDLHISAALPANGAPSSFSLTGTIGLS
                     ESSSTLAAEEPYSVRPAFQFEGEIDTTNLRLREAADFFGPRPVPPQLQGGASLQSRIR
                     VAPGVAGYDVVLSEIAANVDELAVTGKANLAGLLTSQPTFSITFAAPSIDLRSLFTRI
                     PAQWIHAQLPAIIEQRQLGGTVEILSATLTGATAPSPQLSLTGDFRIDKGMALIGNDR
                     VPTQNLAATVSVEPGRIRVGKVTGAYGSLQITDGKALVSFLDDGPWMELDVSGDMSAA
                     DLVKFLTKTIRADKLTSLLAQSRDIEGQTHPTFRLVGPLDKPEGITFAGGEVLAEQVS
                     LMNPSLPQRLTAMRGRIVFSQTGGAQFDQVTANVGEAQLQFNGMISGGTPSVFQDFVI
                     RAKGSASQLRQMVSAGTFPDDLLYGMVNARVQLSGPSGAPHLRGEIGLNEAKLVLPTL
                     GEKPLGSPASLELDADVSKGIGLVISRLELVVPPLRLPLKGRITLGDQFSIDASLATG
                     TVSVSSLPDWVYRSGFEAGNLEVSMDVKGSDAEWKNWRAGGWLALTNGLMTLKGVDGA
                     VEDIYLRLKFSKNIADIKQLSFRIKDSDVSLSGALKNWTTKPVIAVKIESAQMDLDLL
                     IPKGHRSPIREFLETLASTSQVSATATIEKGLYKHLRFGGLSGRLTIQDGMLDLDRVV
                     AQSGTGHAAGRMVVRLPKGEPAETESSVRMTGIPAEAVLQLLGAHDQPVTGDMKLTGA
                     LRGHGRNPHGVLPTLNGKVELVLQDGRILKTEKRAIWKILSILNLPAVLQGKVDLEKE
                     GLQYNRASATLTVQNGLVKTQNIILDSAVLKISAVGTYDMPTDQLDMIWAVSPFGSYS
                     QFLKSIPLFGRLVAGDRKGLATALFQVKGSIDDPQVTYMPMKSFTTGLTGVAQLAFDL
                     LKNTVMLPIDILAPQEEKEPLFDPSLEIQTPPSAPPPVDVPAATAPAAP"
     gene            16858..18144
                     /locus_tag="PLS35_08385"
     CDS             16858..18144
                     /locus_tag="PLS35_08385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI18717.1"
                     /translation="MRRPLNGGTLGESRVGLDTSAVRPRWGILALLFAISAVTYMDRV
                     NISVTARQMMPAYGLTDQDMGYVFSAFVFGYALCQIPGGRLGDRWGARVVLACALLWW
                     SLCTVLTAVVATLPVAGLVGTVGALVIVRFLLGVGESVALPNFNRAVADWMPPGQRGL
                     GIGIAIGGIGIGAAITPPLASWVMVNYHWQSVFYLSALIGLMVALLWVLFSRDGRSDA
                     TAKADTIPTPVPWGTIFRSRPLWWLVASYTCLGYVAYIYMSWLYLYLVNVRGIDLLRG
                     GWLAAGPFLAILLFCPLGGWVTDKLVPTIGLRRARLSIGMLGMALAGGLIAVGAWVES
                     QTLAIVCLSLGAGWLYFTVGAYWSVTTDLSKTHAGTLSGVMNTGANVGGVISPSLTPW
                     LADHWGWTASLLVAAGIALCGGLMWMKVNPEDGLRE"
     gene            complement(18176..>19282)
                     /locus_tag="PLS35_08390"
     CDS             complement(18176..>19282)
                     /locus_tag="PLS35_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HNI18718.1"
                     /translation="LFEPIQVGGLTLSNRIVIAPMCQYSAVDGHMTDWHLIHLGHLAL
                     SGAAALTIEATAVEPEGRITYADTGLWNDATEGAMAHTIESIRRWSDIPLIIQLAHAG
                     RKASTDVPWEGGEQFPPSHNHGWQTVAPSPIPFASGEHPPKALDHTAMARIRESFAAS
                     AQRAGRLGFDGVQIHVAHGYLLHEFLSPLSNCREDEYGGRLEHRMRFPLEVFDAVRAA
                     FRSDRPVTVRVSGTDWVEGGWDIEQTIVFAKALEARGCAGIHLSSGGLDPRQQIPVGP
                     GYQVPLARAVKQAVSMPVIAVGLITDYDQAEEILSRGDADMIALARAILYDPRWPWHA
                     AAHLGGQVKAPKQYWRSQPSRYRDLFLKEHGEAR"
BASE COUNT         3618 a         5730 c         5959 g         3975 t
ORIGIN      
        1 tcggtcttca ccgcccgccc tgcgcgctcc tgatagatat ccaggctcat gtgacagagc
       61 ggacagggcg tcaccatgaa atccgcgccg tgatccttcg catctttcat attcgtgccg
      121 gccatggcca ccgcgatggc ctccttctcc aggataatcg ggaagccgca gcatttggtc
      181 cggcctgcgt aggcgaccgg ctctccaccg acggcgcgaa tgattttttc cagcgaactg
      241 ggattttcgg gatcatcgaa tcccagttcc caggaggggc gaagcatgta gcagccgtaa
      301 aacggagcga tgcgaaactc gcgaaatgat gagcggacag ttccggccac gcgactgagc
      361 ccgagatcgc gtaccgcgat ccagagaaga tgtttcacct gcacgccgcc gccgtaggag
      421 atgccatcct gtgcgaggat cttgttcacc cgttcaagcg tggccggttc gcttttcagc
      481 cgccgattgg ctgcgctcat cacgccctgg caggtcccgc aaatggtcat cacgtccagc
      541 cccatccgct cggcctgcgc gaaggtgcgg gcgttcaagg cgagcgcgag gtccgggtct
      601 gcttccccga tcacccctgc tccgcaacag gacgacgccg cgagttcgac aacctcgatg
      661 ccgaggcggc cgacaatggc catggtggat tggtagagtt caggcgtcgc gcctttcgcg
      721 gcgcaaccgg gatagagggc aaatttaagc ggcatgggcc tcgtcgagta gttgtcctgc
      781 ttgtccttgc accttaccgt attttccccc tcgatgaaca gctcgggcgg tgattcattg
      841 acggggaacc ccgcgtgttg agaaagtcgg ctgtccaggt cagggcagtt tggacaccgt
      901 gtccttataa ctttttttga gatcggccac ggccgcgttg acgttgtctt tcagcgtgct
      961 ccacgcagaa ctggtcgagt gactgacctc gtccagctgc ttgcgggcct cgtccttctt
     1021 gcgctccagg tcctgcaggg ctttctgcat gtccttcctg gcttccacgg aggcggcgct
     1081 ggttttcttc tggagttcag tgatcttcgt ctgcatctcc gccagttcgg cttgaaccgt
     1141 cttttgaaag ctgtcctttt gctggatcgt atactgcttc gtggactcga cggcctcctt
     1201 cgtgtcccgg atcatttttt cggtggtcga cggcggggcc tggtctccgg cagcgatggc
     1261 caagggggcg agaccacacc cgacagccgt gagacagagg accacgagca ctctttctgc
     1321 aatcatggct gggactcctt catgaatgga aacagcctgt aagacaaggg gtgaagccga
     1381 tgcggaatac ctcaaattat ctggcatgtt ctgtgcccgg agccttctcc gcgggaggtt
     1441 aaggttttcg gcgaaatgag ccgaaaagga tctacaacga agacaatccc gcaaccttga
     1501 cctgtggtgt gagccctatg agtgctgaac ttgccccaat gtcagatccg gcctcaacca
     1561 gcccgttaga acagctcttg atcgaacgga tccgcaaagg agccatcgaa ctcccgctgt
     1621 tgccgcaggt tgcctcccgc attctggcca tggtgtacga tcccgatgcc gaggcggcaa
     1681 aactggccgc gctcattcac caagaccagg cgctggccgc ccatgtcatc cggattgcca
     1741 attccccggc gtatatgacc cgtaatccgg tggtttccct ccagcatgcc gtgtccatgc
     1801 tgggcatgaa cctgatgtcc gaattggcct tctctgcctc catcaagggc agcgcattca
     1861 aagtgccggg atgggatggt gaagtcaaga gtctctggca ttattccctc gccagcggag
     1921 cctatgccaa agaaattgcc cgcatgcgcc gattcaatgt cgaaagtgcc tatctctgcg
     1981 gtcttttgca tggcatcggc cggcccgtcg tgttgcagac gctggtcgcc ctggcgaagg
     2041 agcaaggcag tccattgacc aaggagttgc tccatcagct gctcgacgga tattacatcc
     2101 aagtgggcct gctggttgcg gacgaatggg gccttccgcc gcccgtggtg gaatcaatcg
     2161 ggttccacgc cgattatcat tacgccaaaa cggcgaagca ggaatgtatg acgacctgcc
     2221 tggccggtcg cttggcgaaa cattttctgg atcctgaggg cttcgatgaa gccaccgtac
     2281 gagaccattc ggtctttgcc gacctgaacc tgtaccccaa agatatcgat gcgctcctgg
     2341 cccataagga caaggtccaa gccgtcgtgg aggcgatgcc actgtgatgc cggcgaccgc
     2401 ctatgacatc gttgtcgttg gcagcggacc agcgggccag aaggccgcga tccagggtgc
     2461 caaggcaggc aaaaaggtcg tgctgatcga gcaggagccg ggcatcggcg gaaattgcgt
     2521 gtatcggggg acgatcccga gcaagaccct gcgcgagacg gcggtgcagt tcgagcgcct
     2581 gaaacgatcg agtgaagtct tcgaaggcaa aatgcgcatc gatgtgccga tgagcgtctt
     2641 gctgcatcgc ctcgacgaag tggtgaaagc gcatgagtgt tatatggcca gtcagctcac
     2701 gcgcaacggc gtgacctacc ggcacggacg cgcgcggctg ctctcgcccc acgaagtgca
     2761 gctggaaacc gtcgatggcg cctgccaaac tctcaccgcc gaaacgatcg tgctcgccac
     2821 gggatcccga ccgtcgtcga ttcccgagat tccggtggac catgaacatg tgctggatag
     2881 tgattcgatc ctgtcaatga tttacctgcc ccgttcgttg accattctcg gtggcggtgt
     2941 cattgcctgc gaatatgcgt cgacttttgc cctgttgggc gtcgaggtca ccatcatcga
     3001 ccgagccaaa cgtcccctcc ccttcatgga tcacgatatc gtcgacgtgt ttcagcgcag
     3061 catcgagcgt caagggggac ggttttattc cggccatacg gtcaccgagg tggcctggga
     3121 tggtgtctcc tccgtggtgg cccggctcgc gaacggtatg gcagtcaaaa gcgaaaaaat
     3181 gctggtggcg ctgggccggc aaccgaatgt cgaggaattg aatctggagg cggcgggatt
     3241 gtcgctcgat gaaaagggac atatcccggt gaacgatcac gggcagactg cagtgccgca
     3301 catttttgcg gccggcgata tgttgggccg cccgcccgcc ttggcttctc aggccatgga
     3361 agacggccga cgcgctgtca gccatgcttt gggtctgccg gtcggagatt cgctcaatca
     3421 ggtgcccatc ggcatctata cgattccgga aatcgcttcc atcggtctcg atgaggaaca
     3481 ggcggcggcc cgttaccggg gtccgatcat cggccgcgcg ccgtttacgg aaattgctaa
     3541 gggccagatc accggcgcct gcgacgggtt gttgaaactt gtctccgatc cctccggcga
     3601 acgcctgtta ggtgtgcaaa tcgtgggcga gaatgcgacg gaactgattc atctcggcca
     3661 gatggctctg caggatgggg cgaccatcga tcggttcatc gacaccattt tcagctttcc
     3721 cacgtttgcc gaagcctatc gcgtcgccgc cctcgacatt ctgggccagc gccgtaaacg
     3781 gcgggacgag gctcaagcag cctgatcgtc catccttctc tcgccgcttc ctttgacccc
     3841 cgtcccccat ctgcgctact ctctccatcg gccggtcttc cgatcgatgg aggttgtaca
     3901 tgaatcgtga ggagcgtcgc cgcgaagaaa agctacaggg gcgcaatgcc ggaggcccgg
     3961 cagcggcgtt ggccgggttg cgcgaggccc agatgcatca tcaggccggc cgcctggatg
     4021 acgcagaacg tggctatcag caactgctgg cacggatgcc ggcgcagccg gatgccctcc
     4081 atggtttagg cctgctgctg taccggcgcg gcaatctccg tgacgcgctc actcgcctgg
     4141 cccaggcacg cgcggccgat gcgcgcaatc ccgtctactg tttcaaccat ggcgtcgtgc
     4201 ttcaacgagc agggcttctc cctgacgcag ttgatgcata caatcgagcc attcagctca
     4261 acccccggta catcgacccc cgcacgaacc tcgggaacgc ctataaagag ttagggcgcc
     4321 tggcggaggc ccaagctacc tatgaacagg tgctggcgct caatccggat catgccgaag
     4381 cgcacaacaa tcttggcgtc gtgttgaaag aacaaggccg gctcgatgcc gcggccgatg
     4441 cgtaccgcaa ggccatcgcg ctgaaaccgg cgcatgccga ggcccacaac aatctcggat
     4501 tggtcttact ggagcagggc ctggtggatg acgcgatccg cagcttcgag cgtgcgctgc
     4561 aggcccatcc gggctacggg acggcactgt acaatctcgg gatcgcgtgg atctggcggg
     4621 aagatattcc tcgcgcgctg cactgctttg cggaaacggc caaggccaag tgtgcacacg
     4681 gccgccccgt caccgactcc gcggtctttc ggtcgcgtgt gaagcacgat gccgagcaaa
     4741 tcggctacct gtttgaacgc gggttaatcg gcgaggaatg gcgcgggtat catgacgcgc
     4801 ttctccagtt gtgcgggcgt ctcgagccgg cccccggcac cgtctcagcc aatcgtgttg
     4861 ccctctcccc tgccgacctt cagcctatcg ctccttcatt caaccggctc ctgtacattg
     4921 cgccttgcga cacgattgcc gaaggggcgc tggcgccgga tcttgatcac gcggcgatcg
     4981 aagcacggta tctggcgacg cagccggagg tgacctacat cgatgggctc ctgtcggagg
     5041 aagccctgcg gcgtttgaga gaattctgct ggacgtcgac gatctggaag aaggactacg
     5101 agaacgggta catcggggcg tttctcggcg acggctttgc gtcgccgctg ttgctccaga
     5161 ttgcggaaga acttcgccgg cgtctgccgc gtattttcgg cacgcatcgt ctcacccagg
     5221 cctgggcgtt caagcacgat agcgcccggc gcggattaaa catccatgcc gatgccgcgg
     5281 cggtgaacgt caatttctgg atcacgccgg acgacgccaa tctcaatccc gagagcggcg
     5341 ggctggtggt ctgggacaag gaagccccga gggagtggga cttcaaaacc tacaatagcg
     5401 acaaggcgcg gggcagcatt tacgagtggc tcacgtcgca aggggcgaag gaaatcaaga
     5461 ttccctatcg ggccaatcga gccgtggtgt tcaattcaga tctgtttcat gaaaccgacg
     5521 acattgcgtt caaggaagga ctcaccaacc ggcggatcaa cattacgctg ctgtacgggc
     5581 atcggcatcg cccttgagca gtttttcgaa atcttcggcg caaacagggc ggctgaacag
     5641 atatccctgc acctcatcgc atccttcttc ccgtaacttc gccaactgac cttccgtctc
     5701 cacgccttcg gccagcaccg acagattcaa gctgtgggcc atggcgatga tggcgcgcgt
     5761 gatcttcaca ttgttctcgt tcgtcagcaa atcccggacg aaggattgat cgattttcaa
     5821 acggctcagc ggaaaccgtt ggagataact caacgaggaa tacccggtgc cgaagtcatc
     5881 gattgagagg cgcaccccca tggtccgcag tccctccaac atggtgacgg aggcttcgac
     5941 gtcccgcatc gctatggact cggtcagttc gagttcgagt tgcgcgggat tcaagccgga
     6001 gtcccgcagc gcatcagcga cggtggtagg cagcgtacgc ccgtggaaga gcgagttgga
     6061 gacattggcc gaaatggtga tcgggggaag tcccgccttt tcccaggcct tgacctgacg
     6121 acaggcctcc cggagcaccc attcgtccat ggagcggatc aagcccgtgt cgatggcggc
     6181 gttcaagaac actcccgggg gaaccaggcc gcgcttggga tgtttccatc gcaccagcgc
     6241 ttcggctccg aggatgcggc gcgagtggat attcagtttc ggctgataga agacgacgaa
     6301 ctcttcccgt tccagcgcgc gccgcagttc gttctcgaga tcgaggcgtt cggccgccgc
     6361 ggcattcagt cccgacgaat agtactggca attgttccgg ccctgttcct tcgcatggta
     6421 catggccgtg tcggcgttct tcaggagcgc ctcgaccgtg gagccgtccg atgggaagat
     6481 cgaaatgccg atgctggcag agataaagat ttcatggccc tcgatgctga acggatgggc
     6541 cagcgattcc aggattcgcc gggcgaccct gcctgcatct tccggctgcg gtaaggccgt
     6601 taacaggatg gtgaattcat ctccaccgag acgcgcgagg gcgtgcatcg gttcctggtc
     6661 ggcatggcga ctgaccgagt cactttgccg aaccgattcg ctgagacgtt ccgcgacatg
     6721 tgtcagcaac cgatcgccca ccgtatgtcc cagcgtgtca ttgatgatct tgaagcgatc
     6781 caggtcgata aacagggtcg ccagatgctg ctggtggcgc gatgcatagg agatggcatt
     6841 cgacagacga tctttgaaca acacgcgatt ggctaggccg gtcagactgt cgtaataagc
     6901 gagctgatga atggcccgct ccgcttgctt ccgctcggtg atgtcctggg ccgtcccgat
     6961 cacggtgatt tcgtcggtct cttcttcgcg cacgccttcg gcttggagat ggatcacgaa
     7021 gtcggtgccg ttgggcagta cgatgcgatg atcgatatcg cagggcgtat gttgcgcaac
     7081 gagtttctgc aacgcacccg tcacgatcga acgatcgtcc gcgtgcacga gggcgagaaa
     7141 cgcctcgaag gtaccggcaa aatcctgcgg ccgcaccccg accaggcggc acaattcatt
     7201 cgacatggcg aaacggttgg tcttcggatt ccagtcccag ttgccgatgc gagcgatgcg
     7261 ttgcgccagt tccagccggg attcgctgcg gatcaacgcg tgcagcacgt tattggtgcg
     7321 aagcatgtat cggacgtggt ggcagagaat cgtggcattg accggtttgg tgatgaagtc
     7381 ggtagcgccg tgctggtagg cctgtccgat ggactgcgcg tcatccaggc cggtgaggac
     7441 cagaatgggc actcgtttgc ctcgcggaag cgagcggatt cgggaacagg tcagatatcc
     7501 gtccatatcc ggcatcatga tgtcgagaat cacgagatcc ggcccgatgg cttcaaactt
     7561 cgtcagcgcg tctttcccgc ccgtcgcctc caggacgatc atgccggccg gttccaatgc
     7621 gtttttgacg aacagccggg cgagtggatc atcgtcggcg atgaggattt tgggagtcgt
     7681 ttgactcata tggtgtctgg cctataagtc tcgcggtcgg ttgattcgcg tcgccgctcc
     7741 ggggcacagg ccttgtgcga gcagtggccg gcctgccgat tcagcgttcc ccacagccta
     7801 tcaaactttg gaagaactgc cagagaatta gcacttgtcg agcatctcgc gtgcaggtcg
     7861 aattcgtagg tgaaagcctg agaggaacgg tatagccgga atggttcagg tttcacgcca
     7921 tctcgcctct ctcggtcgtg tcgaggcccc cgggtatcgt gtgagggcct tgctctctgg
     7981 cttcgaggac aatgccggcc aagagcgtgc ggacccggct catcaggccg cgcatcgtca
     8041 gcatcagaag agttgcggtc aacagcagca gcggggcgat caccagggcg acatcccggc
     8101 ctaaatcatc gtccgatcgg ggagcggccg cttcaggcgg tgtcgcgacg gccagtcgta
     8161 tccaaccagc cacccggttg tcccggcgga atggttccat gacgatgaag gcagctctcc
     8221 cctgttcgat accagacgtg atgacaccgc tttgtgtccg gcgtgctgcc agcgaggccg
     8281 cgtcttggag cgtggttcca atggcggcgg gattgctcga tgcgaccacc acattgttgt
     8341 ccatgatcac ggccgcggcg aggagattgg cctgctgagc atgttgctcc agtgtctgct
     8401 ggatggagtc gaaattatcg ccgcccatcg attcgctgaa ccaggccagg gtttgcgccg
     8461 tggccctggc gctgtactct gccgccatga cggccgaagc attccgctgc gccacgaggg
     8521 agcggtcatg ctgagccaac agcacataga gggccgcggc gcacaatgtg gaaatgatga
     8581 tgctgaccag caacgccggg agccaggtcc gaggcaatcg cgatgcggaa gcgtcagcca
     8641 tgctatatcc tcctcgacag gaaggggcgc taggcgccgg agccttcctg cgcgtcctga
     8701 gtccgttcgt agggatggcg agtgagtgct ttgacgatcc gttgaagccg ggcccattcg
     8761 tcaggcggca gggactgaaa ttcaagaccg atgcggccgt cccggcacca gcgtaccacg
     8821 gcttgctgga cggtcagcat ggtctctgga tccggaagat gaatgcggag ggtcagtgtg
     8881 gtgccgggat gcatggcggt ctgactgacg atcccgcatc ctcgcaagga gagatcgacc
     8941 acggtgccgt ctccctcgac gcgattcact gaactgaagg cacttcggaa gtgagcggga
     9001 aaccgttcgt gctggcgttg ttccatcgtg aggcggcgtt gagtgaatga cgacacgctc
     9061 tctgtgctgc gttccccgga agccgcgcat gtgtgtcgaa tgcatgccgt gctagccctt
     9121 tatattgcca attggtttga gttccgccac tttttttgtg attcccgcct ggtccaccgt
     9181 gttcaccacg tcggaaatgt tcttgtaggc cagtccggcc tcttcagcga ggcccgacat
     9241 cgacacagcc ttgaccagaa taccctgttg tttcatgtcg cggagcagtt gttcgccgcg
     9301 taccgagcgt ttggcctgtg cgcgggacat ggtgcggccg gacccgtgca tcgtggagcc
     9361 gaaggtgtca tgcatggcgc gattggttcc gaccaggaga taggagccgg tttccatcga
     9421 gccaccgcaa atgaccggct ggccgatcgc gcgataacag tcgggcagtt ccggactccc
     9481 cggcccgaag gcccgcgtgg cgcctttgcg gtggaccagc cactccccgt ctccgtaacg
     9541 ctccaccttc gcgatgttgt gtgcgacatc gtagatcaga tgcatgccga gtgcatgcgg
     9601 ggtggaatcg aacacggcgg cgaaggcttc gcggatttga tgggtgatga cctggcgatt
     9661 ggcaaatgcg gtgttggcgg cgcagttcat cgcgccgaaa tactcctgcc cttccggtga
     9721 acgaaacggc gcgcaggcga gttgttgatc cttgacggag attccgtagc ggcgcatcgc
     9781 cttctcgaag actttgaggt agtcgctcgc gacttgatgg ccgaatccgc gggagccgca
     9841 gtggaccatc accacgactt ggtcccggcc ggtcaggccc atcgcggcgg cggtctcccg
     9901 atcgaagatg tttttgtctg agacgacctg cacttccaga tagtgattgc ctgatcccaa
     9961 tgtcccgagt tggttgatgc cccgttcgat ggcgtaatct gtcacatgcg ccggatcagc
    10021 gccgtcgagg caaccccgtt cttcgatccg ttccaggtcc tcgtcccacc catacccctt
    10081 tgcgacgcac cagacagcgc ctttgcgcat gacctcccga aactcgcgcc ggttcacgtt
    10141 cacgaagccg cgcgatccca cccccgccgg gacgcggcgg aacaactcgg tcatcagcag
    10201 ttccagtttc ggttgcacct cggcgcgcgt caaatccgtg cggatcaaac gcatgccgca
    10261 gttgatgtca tagccgacgc cgccgggaga gatgatgccg tcctccggat cgaaggccgc
    10321 gacgccgccg ataggaaacc cgtagcccca atggccatca ggcatgcaga gcgcataacg
    10381 atggatgccg ggcagacagg cgacgttggt gacctgatcg aagacgccgc gatccatggc
    10441 tccgaggatg gccggactcg cataaattct tgcgggaacc agcatgcccg ccttttcgga
    10501 tggcggaatt tcccagagat agtcgtcgat acggttcacc tgcatgtcgg tgttgagtgt
    10561 catcggttca cctgtcaatt gcacaagaat tgtgagggat gtggtcgttc atcagacatc
    10621 caagaccact ctggccttcc aactatttcc ggtttgcgtc acggcataca agtgtttggt
    10681 gacacccttg atgtcggatc ggagatcctg cgtggcctga tcgaccgggg ccccgaccag
    10741 ttctgcatgg aggtgccagg cggattgacc gggcgctgcc tgcaccgtca acgtggcatg
    10801 gtgaaagacc acggcttgcg cgtctttcaa gtacacgagc cggttcagcc attcaaagag
    10861 cagcgtatcg atgtcaggtt cttccatgtc gacgacctgc tgccaggtct gcgcaacggt
    10921 agcaggatcg gccaggcttt ggaggagcgc ctgcgttgcg gcatcgaaaa gcgacgcaag
    10981 agaatcgccc tcggcctcga aggccatgtc ggccatcgcg atgtcgtcga gaaatcggaa
    11041 ggtgcccggc atgggcgtca gacgcgcgtg gtccgtcgtc gggctgcagc gaaaattgcc
    11101 cggacctgtt cgatccctgg aatggtatgg ccgaacggca atgggacctt acctttgaaa
    11161 aacatgcgga ccccgagcgg catgacatcg accacgcgtt gcaggttaaa tccgagaact
    11221 ttgagcggca tcaaggcctc gttcaaccgt ccttcctggc ggacgaggtc gacaaagccg
    11281 gtaatgtgac gggcgccctc ggcagtggtc aaaccatgcc gaagcgaaga ccgccgcagc
    11341 cggatgatcg cctccatcgg ttgaacgtct ttcgggcaga cctgcacgca catgttgcac
    11401 cgcgtgcaat cccagatgcc gtcggcttct tgaagcgcgg cgagccgaac ctgtttcgca
    11461 tccgccggtt cgcgcgggtc ggccacgaag cgcgccgctt tggcgagcgc cgccggaccg
    11521 agaaagccgc gggagacttc atgggaggtg caggcagcaa cacaagccgc gcacatgatg
    11581 caggcgtcta cgttgtgaaa ttggtaactg tccggcagca ggcgcagttg ccctgaggat
    11641 ccgtagcgtt tggtcgggtg cgtgatcggg gtaagccagg gcgtgaccgc ccggatcttt
    11701 tcccagaagg gcgccatatc gaccaccaga tccttgatga gcggaagatt cggcaaggga
    11761 gcgatgctga ttctgccatg ccgctccagt tctttccgga tggacgtacg gcaggccagc
    11821 ttttgtgtgc cgttgatcag catggcgcag gagccgcaga tcgccgaacg gcaggaatag
    11881 cgtagcgcca ggcggccgtc gagttcgttc ttgatgcgga tcaaggcttc gagcaccgtc
    11941 atgcctcgtc cgatgtcgag gcggtactct tcctggtgcg gctgctggtc ggtttcggga
    12001 ttgaatcgct ggatcgtgaa ggtgaggcgc ataacgttgt gaacgtcgag atgcgaatac
    12061 cgtaggcggt agacgcctaa ccgagttcaa acactttcgg atagttcgtc agattccggc
    12121 aaccgtcttt cgtgacgagc accatgtctt cgatgcggac ggcgccaagt cccggatagt
    12181 agagccccgg ttccaccgtg acgacatggc cttcctgaag caatgaaccg gtccggctga
    12241 tccgaggcgc ttcatgaatg tccagtccca cgccgtgccc ggtgccgtgg aagtagcctt
    12301 gcatgcgacc gttcaccacc ccggtcttgt agccggattt ttcaaaccgg tcgcagatac
    12361 cctggtggat gaccgccccg tcggccccgt cgcgaatttt ggtgatggct tcttcctgcg
    12421 cgtccttgac ggtctgatac agcttcttca actcgggaga aggctgcccg cgcaccaccg
    12481 tgcgggacat gtcggcaaaa tagcgggaat cggcggagcg cggaaacacg tcgaaaataa
    12541 tgctgcgatg cgccggcaac gggccgctgc cttcgtcgtg cggatcgcag gcctgttctc
    12601 cgccggccac gatggtatgt tgcgccacgc aatcacattc catcagggcc acgttgatga
    12661 gtttcttcac gcgttccgac gtgagcacct gcccgtcgag ccagaggaca ttgtcgcgaa
    12721 tctcggcgcg gcgcaagctc tcgtgggcag cggccacggc cttctcggtc gcgcgctggg
    12781 ctgcttcgat gtgccggact tcttcgtctg acttcaccac ccggctttca taaaacggat
    12841 cgcgtttcgt gcgtagccgg tatccaagct cctggagtcg ttgcgcatgg agaaagggaa
    12901 aagtcggtgg caccaggata tcgcggacgc cggcgtcctg caggaccaga tggatgacat
    12961 cgatattgcc gggatctttg acgccctgtg ctttcgcgcg tcgctccagt tcggaatagg
    13021 acaccacgcg atcaaccgtc gcttgatgcc gcgcacgatc gacctccaga tcactcatga
    13081 tcatcagccg ctcccctttc acctcaaggt agatgaaggg atccggcgcg atgaatttcg
    13141 tcgcgtaata gagattggaa tcggtttcgc tggcggcaat gaagactgtc gcgccgggga
    13201 cgggattcgt atgctggtcg gtggaatggc tcatgaggtc gaggcgatct aatcaatcgg
    13261 atgctatcac gcgctccgga ggtggtcaag gcgcggccgg cgccgtcgca gccggaacat
    13321 cgacaggcgg cggtgcggag ggcggtgttt gaatttccaa tgacggatcg aacagcggct
    13381 ccttctcctc ttgcggtgca agaatgtcga tcggcagcat gaccgtattc ttcagcaggt
    13441 cgaaggcaag ctgcgccacg ccggtcagac ccgtggtaaa ggacttcatc ggcatgtacg
    13501 tgacctgtgg atcatcaatc gaccccttca cctggaacag ggccgtggcg agacctttac
    13561 gatcccctgc caccaaccgc ccgaagagcg gaatcgattt caagaattgt gagtatgatc
    13621 cgaacgggct gaccgcccag atcatgtcga gctgatccgt gggcatgtcg taggtgccga
    13681 cggccgaaat cttcagcacc gcgctgtcca gaataatgtt ctgcgttttg acgagcccgt
    13741 tttgcaccgt cagcgtggcg ctggcgcgat tgtactgcag gccttccttt tcgaggtcca
    13801 ctttcccttg caggacggcg ggcagattca ggatggagag gattttccag atggcgcgtt
    13861 tctctgtttt gagaatgcgt ccatcctgta gcacgagttc cactttgccg ttgagtgtcg
    13921 gcagcactcc atgaggattg cgtccatgtc cgcgcagcgc cccggtcagt ttcatatcgc
    13981 cggtgacggg ctgatcgtgc gcacccaaca attgcagcac ggcctcggcg ggaatgcccg
    14041 tcatacggac agaggattcc gtttcagccg gttctccttt gggaaggcgc accaccatcc
    14101 gccctgccgc atgtcccgtt ccggattgtg cgacaacccg gtcgagatcc aacatgccgt
    14161 cctgaatggt caaccggccc gacaatcccc cgaaccgcaa atgtttgtag aggccctttt
    14221 caatcgtggc tgtcgcgctg acctgactcg tggaggcgag tgtttccagg aattcccgga
    14281 tcggtgagcg atggcccttc gggatgagca gatccaggtc catttgcgcc gactcgattt
    14341 tgaccgcgat caccggcttg gtcgtccaat tcttcagggc gccggacagg ctcacatcgc
    14401 tgtccttgat gcggaacgaa agctgtttga tgtcggcgat gtttttggag aacttcaagc
    14461 gaaggtagat gtcttctacc gcgccgtcca cgcccttgag cgtcatcagg ccattggtca
    14521 gcgccagcca gccgccggcg cgccagtttt tccactcggc atccgaacct ttcacgtcca
    14581 tggacacttc gagattgccg gcttcgaagc cgctgcgata cacccagtcg ggaagactgg
    14641 agaccgatac cgtgccggtg gcgagcgacg cgtcgatgga aaactgatcg ccgagggtaa
    14701 tgcgtccctt caacggcagc cgcaagggcg gcacgaccag ttccaggcga gaaatcacca
    14761 ggccgatgcc tttgctgacg tcagcatcca gttccaggga ggccggcgag cccaacggtt
    14821 tttcacccaa cgtgggtagc accagtttgg cttcattcag gccgatctct ccgcgcaagt
    14881 gtggcgcccc ggacggcccc gagagctgca ctcgcgcatt gaccatgcca tagagcaggt
    14941 cgtcgggaaa ggtgccggcc gacaccatct gccgcagctg cgaggcgctg ccttttgcgc
    15001 ggatgacgaa atcctgaaac acgctcggcg tacctccgct gatcatcccg ttgaattgca
    15061 gctgggcttc ccccacgttg gccgtgactt gatcgaactg cgcgccgccg gtttgcgaga
    15121 agacgatacg gccgcgcatg gccgtgaggc gttgcggcag cgagggattc atgaggctga
    15181 cctgttcggc cagcacctcc ccgcctgcga aggtaatgcc ttcgggtttg tccagcgggc
    15241 cgaccagccg gaacgtcgga tgggtctggc cttcaatgtc gcgggactgc gcgagcagcg
    15301 aggtgagctt gtccgcacga atggtcttgg tgagaaactt caccaggtcg gcggcgctca
    15361 tgtcgccact cacatccagc tccatccagg ggccgtcatc gaggaatgac accagggctt
    15421 tgccgtcggt gatctgcagt gagccgtagg cgcccgtcac tttgccgacc cggatgcgtc
    15481 ccggttccac tgacacggtt gcggccaggt tctgcgtcgg cacgcgatca ttgccgatca
    15541 gggccatgcc cttatcaatg cgaaaatcac ccgtcaatga caactgcggg ctgggcgccg
    15601 tggcgcccgt caaggtcgcg gagagaatct cgacggtgcc gcccaattgg cgctgctcga
    15661 tgatggcagg cagttgggca tgaatccatt gcgcgggaat ccttgtgaag agcgagcgga
    15721 gatcgatcga tggcgccgcg aaggtaatgg aaaacgtcgg ctgggaagtg agcagtccgg
    15781 ccagattggc cttccccgtc acggccagtt catccacgtt ggcagcaatc tcggacagca
    15841 cgacatcata gccggccacg cccggcgcca cccggatgcg gctttggaga ctggcccctc
    15901 cctgcagttg cgggggaacc ggccttggcc cgaagaaatc cgccgcttcc cgcaggcgaa
    15961 gattcgtggt gtcgatctcg ccttcgaatt gaaaggccgg gcgaacggaa tagggttcct
    16021 cggccgccag cgtcgaagac gattcactca atccgatcgt gccggtcaga gaaaacgaag
    16081 agggcgcgcc attcgccggc agcgcggcgg aaatatgcag atccccttct gccttacccg
    16141 gatagacctt cagcgccatc tcgaccgttt cgagcgtcgt cgtccgcacg ccgtccggcc
    16201 gtgcctcatc cgtcaccgtg atgtgcccct tctggatggt cgcttcacgg atctgcagca
    16261 gccggctgaa catctgatac gccgactcgt ctttcgccgc cgagggcatg cccgccagga
    16321 cattccaatg gcccgatcgg ttgcggacga gcgtcacggt cggttcgttg agaaagatgc
    16381 gcttcgcgac gacctgcttt ttcagcagcg gcagcaggcg caggacgata tcgatttcct
    16441 tggccgagaa gacgacgtgt gtggggtcgt cgtggccgta aatgccgacg ttggtgagct
    16501 ccaggcgaat gctgggcagc accacgagtt tgatgcgatc gacttcgatt ttacggcgaa
    16561 ggctggtttc gagttgctgg aggaagaagt tcttgaggat gtcgacgccg aacagttcgc
    16621 gcgagtagaa gagaagcagg atccccgcga ccaccaggcc gagcagcgac agcactaccg
    16681 tgcggggacg aaccctcact gaccctcctc taagtagagc aagaacgcag tgtactggat
    16741 gcgtacgcgg gaaatccata cacaggcgtg cggcagaggc gcaaaacctg agcgagacac
    16801 ctcccatgtc gcggtgcctc gcctttcaca tttcagtcgt gcgtgtacgg ggctataatg
    16861 cgccggccgc tgaacggcgg gacactgggg gaatcacgag tggggctcga cacatcagcg
    16921 gtgcgaccac gttggggcat tctcgctcta ctgttcgcga tcagcgccgt cacatacatg
    16981 gaccgggtca acatttcggt cacggcccgg cagatgatgc ctgcctacgg actcaccgac
    17041 caggacatgg gctacgtctt ctcggccttc gtcttcggct acgcactctg ccaaattccc
    17101 ggcggacgcc tgggcgatcg ttggggagca cgagtggtgc tggcttgtgc cttgctctgg
    17161 tggtcgctct gtacggtgtt gaccgccgtg gtggccaccc tgccggtagc cggtctggtc
    17221 ggcacggtcg gcgcgctggt catcgtacga ttcctgctcg gcgtgggcga atcggttgcc
    17281 ctgccgaatt tcaatcgtgc tgtggctgac tggatgccgc cgggacagcg tgggcttggg
    17341 atcggcatcg ccatcggggg catcggcatc ggtgcggcca tcacgcctcc cctcgcctcc
    17401 tgggtcatgg tgaactatca ctggcaatcg gtgttttatc tctcggcctt gatcggtctg
    17461 atggtggcct tgctgtgggt gctcttctcg cgagatgggc ggagcgacgc aacggcaaag
    17521 gcggacacca ttccgacgcc ggtcccctgg gggacaatct tccgctcccg gccgctctgg
    17581 tggctcgtcg cgagttacac ctgtctcggc tacgtcgcct atatctacat gagctggttg
    17641 tatctctacc tggtgaatgt gcgcgggatc gatctcttgc gcggcggctg gctggcggca
    17701 ggaccctttc tggcaatcct gctcttctgc ccgctcgggg gatgggtcac ggacaagctg
    17761 gtcccgacga tcggattgcg cagggcccgg ctttccatcg gcatgctcgg catggcgttg
    17821 gccggtggat tgattgccgt tggtgcatgg gtggaatctc agaccttagc gatcgtctgc
    17881 ctgtcgctcg gtgccgggtg gctctacttt accgtgggcg cctattggag cgtgacaacg
    17941 gatctctcta agacccatgc cgggactctc tctggtgtga tgaatacggg cgctaacgta
    18001 ggcggcgtga tttctccgag cctcacgccc tggctcgcgg atcactgggg ctggacggcg
    18061 tcactactgg tggctgctgg tatcgcactc tgcggagggc tcatgtggat gaaggttaat
    18121 ccggaggatg ggttgaggga atgagttctt cccctcaatg ttgcattctc atctgctatc
    18181 tcgcttcccc gtgttccttg agaaataaat cccgatagcg gctcggttgg ctgcgccaat
    18241 attgtttcgg ggccttcacc tgacctccca gatgggctgc agcgtgccac ggccagcgcg
    18301 ggtcgtagag aatggctcgt gcaagggcga tcatgtccgc atcgcctctg ctgaggatct
    18361 cttcggcttg gtcgtagtcc gtgatgagac cgacggcaat caccggcatg gacaccgcct
    18421 gcttgaccgc acgagcaagg ggaacctggt agccggggcc gactggaatc tgttggcgcg
    18481 gatcgagccc gccgctgctg agatgaatcc ctgcgcaccc ccgtgcttcc agggccttgg
    18541 cgaacacaat ggtttgctcg atgtcccagc cgccttcaac ccagtctgtg ccggacacac
    18601 gaaccgttac aggccgatca gaccggaatg cggcacgcac ggcatcaaac acttcgaggg
    18661 gaaaccgcat gcgatgttcg aggcgcccgc cgtactcgtc ttcccggcag ttcgaaagcg
    18721 gtgagagaaa ctcgtggagc agatatccat gggcgacgtg aatctgtact ccgtcaaagc
    18781 ccagccgacc ggcgcgctgg gccgaagcgg cgaacgattc gcggatgcgc gccattgcgg
    18841 tgtgatccag cgctttgggg ggatgctcgc cgcttgcaaa cgggattggc gacggggcga
    18901 cagtctgcca cccgtgattg tggctgggag gaaattgttc ccctccttcc cagggtacgt
    18961 cggtcgacgc ctttcggcct gcatgagcca gctggatgat gagaggaata tccgaccaac
    19021 ggcggatgct ctcgatcgtg tgtgccatgg caccttcagt ggcatcgttc cagagccctg
    19081 tatccgcata ggtgatgcgg ccttccggct caacggcggt ggcttcgatc gtcagcgccg
    19141 ccgccccgga gagggcgagg tggcctaggt gaatgaggtg ccagtcggtc atgtgcccat
    19201 cgaccgccga atactggcac atcggcgcaa tgacgatccg gttgctcagc gtgagcccgc
    19261 cgacttggat tggttcgaac ag
//
LOCUS       DAOQEW010000059        19050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_736_length_19050_cov_8.290813, whole genome shotgun sequence.
ACCESSION   DAOQEW010000059 DAOQEW010000000
VERSION     DAOQEW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_736_length_19050_cov_8.290813"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLS35_08395"
     CDS             complement(<1..310)
                     /locus_tag="PLS35_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HNI18719.1"
                     /translation="MNKIEAIKTERDGLAVRDMLEHYAHAGWESIPEDDIQRLKWYGL
                     FLRNPTPGFFMLRVRMPGGQTTSVQLQALADIALAYGNGVMDVTTRQQVQLRHLTIAH
                     V"
     gene            complement(307..504)
                     /locus_tag="PLS35_08400"
     CDS             complement(307..504)
                     /locus_tag="PLS35_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18720.1"
                     /translation="MKTNEETVVLTLLRRQAPQSFEQLMALSGLSASQVLLVVDRLSR
                     EGTVVLRKSGLDYSVALGSTI"
     gene            complement(554..997)
                     /gene="cynS"
                     /locus_tag="PLS35_08405"
     CDS             complement(554..997)
                     /gene="cynS"
                     /locus_tag="PLS35_08405"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HNI18721.1"
                     /translation="MMEKEAMRKAIKAQRLKKKVTIAQVAKKVGKNPTFVAAALNGNH
                     RLVPADAAKVGALLGLNQEQVAALSAFPIRADFPNLTDPFKYRLMEVIGVYGDSLREM
                     ANEMFGDGIMSAIDFTLDMEKVTGSQGEARCKITLNGKWLEYKTF"
     gene            complement(1048..1878)
                     /locus_tag="PLS35_08410"
     CDS             complement(1048..1878)
                     /locus_tag="PLS35_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNI18722.1"
                     /translation="MHTADHERIAGVAAKKWMFLEQAPGGYFILSSLAGIYLGFGIAL
                     IFSLGGPLAAAGSPVVKLVMGVSFGIALTLVIFAGSELFTGNNLIGAIGGLSRSLSWT
                     QVIQLNAWSWFGNLAGSMGLAWLIVESGVFAKGPSADLIEKVAAAKMSLPAWELFVRG
                     ILCNWLICLAVWMTGRTTNDTAKILLIFWCLFAFIGTGFEHSIANQSLLGMALFLPHD
                     VTVTWAAFWYNQLFVVLGNLVGGGLFVGGLYWMVSPYRVAEVPVTPVSSPATSAVVGS
                     "
     gene            complement(2196..3605)
                     /gene="glnA"
                     /locus_tag="PLS35_08415"
     CDS             complement(2196..3605)
                     /gene="glnA"
                     /locus_tag="PLS35_08415"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNI18723.1"
                     /translation="MNVREVLDFAKKNKVQVVDMKFVDLIGTWQHFTIPVSELTEGLF
                     KDGSGLDGSSIRGWRAINNSDMLLVPDPATACMDPFCSVPTLSLIGNVVDPITREMYD
                     RDPRFIAQKAEKYLQSTKIGDTSYWGPEAEFFIFDHARYDQTNHSAYYHIDSDEGVWN
                     MGQDGVNLGGKIRHKEGYFPVPPTDTQQDIRTEMILEMEKAGIATEKHHHEVATAGQA
                     EIDIRFDSLLRTADKMMMFKYIVKNVARRHGKTVTFMPKPIFGDNGSGMHTHQSIWKD
                     GKPLFAGKEYAGVSQMCLHYIGGILKHAPALAAFTNPSTNSYKRLTPGFEAPVLLAYS
                     SRNRSAGIRIPMYSPSPKAKRIEVRFPDPAANPYLAFAAMLMAGLDGIENKINPGEPA
                     EKDLYDLEAKEAAKIRTMPGSLDEALNHLEKDHQFLLKGGVFSEDLIEAWIGYKRTKE
                     VDTMRLRPHPYEFFLYYDV"
     gene            complement(3655..6339)
                     /gene="glnD"
                     /locus_tag="PLS35_08420"
     CDS             complement(3655..6339)
                     /gene="glnD"
                     /locus_tag="PLS35_08420"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNI18724.1"
                     /translation="MSHALDAGFHMVQDAAAIPVSQLLAEQRQAIQQRLLAGASGDEV
                     VAATTDLVDGLIIGRYRTAARTGGEALTTAGFQHCCLVAIGGYGRRELAPHSDIDLMF
                     LFHQDAAKVIPELVKQVLHPLWDIGFQVGHSVRTIQDCIDLGLADLTVRTSMMEARFL
                     TGSPELFQQFHARYVRKVVSSGFDGYLEQKVAERQREYQKFGETVYLLEPNVKKSKGG
                     LRDLHLLQWIGSARFQAPTIRELSDRGILSQSDYRSIKEAREFLLRVRSFLHIRAGMA
                     QEILTFDEQVWLAKHLGFTDRPHLLAVEQFMQQYYRHTMGLHVALMRFVERCRRRSLW
                     QRLVRWLPSPRIQQYFSVDGEALTVPVELRSQVLVQPTLLLTLFNLARSRQLSIDPSL
                     LEDIHRHAETLSVEQFHTPEAGQTFLSILAGPGTARTLEAMHRAHLLEKLVPAFSRVR
                     GLMQFNQYHKYTVDEHSLLAVAKAETLADHSGVLGEVYREIKRKDLLHLALLLHDLGK
                     GHEEDHSEVGKRLAEETAARLGFDEPDTRTLVMLVHRHLLMAHTAFRRDPYDEKVLLP
                     FAREVGTPEVLRKLLALTAADIAAVGPDVLTKWKESLLVELYLRAMQEVSGERETADE
                     PQRLARIADDVAGQSQGDHNLPADKTWICSELEQFPLRYVYGTSPLRIAAHLGAIRRL
                     QEGGVVVETEFNAPLGTCEYAVIAHNDLIPGLFSKIAGVMAAGGLQILDAQIVTRKDG
                     VVVDTFQVADPDYQGAPPADRCESIGATITEVLTGRQSIDAVMRRGARLNLGRSLPAH
                     RQPAEVRIDNETSDRFTILDVFADDRQGLLYIITNAIFQLGLSVHASRISTRLDQVAD
                     VFYVTGMDGKKVEDAGRLETIRASILNEIELFLGAHAA"
     gene            complement(6342..6680)
                     /locus_tag="PLS35_08425"
     CDS             complement(6342..6680)
                     /locus_tag="PLS35_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNI18725.1"
                     /translation="MKLIEAIVKPFKLDEVKDALLEIGIQGMTVTEVKGFGRQKGHKE
                     TYRGQEYTIEFVPKVKIEVAVNDGQVQRVLETITRAAKTGSIGDGKIFVRDLASAVRI
                     RTGETGESAL"
     gene            complement(6727..8055)
                     /locus_tag="PLS35_08430"
     CDS             complement(6727..8055)
                     /locus_tag="PLS35_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNI18726.1"
                     /translation="MKASAQWKTFLVGLAGALTLIGGLGAEQAWAENAPLKADSGDTA
                     WVLVSSAFVLAMLMPGLALFYGGLVRTKNVLGTIMQSIMILSVVSLLWILFGYSLAFG
                     PDKGGVIGGLDWIGLSGVGSQPHAVYAPTIPHQAFMLFQLMFAAIAPALITGAFAERK
                     RFTSVVLFAAMWSVLVYVPLAHWIWGGGWLAQLGALDFAGGAVIHISSGAAALICAIV
                     LGKRRGYGTDYMAPHNLPMTLLGTGFLWFGWFGFNGGSALGANGVAVSAIIASHAAAC
                     MGAISWCGAEWMHRGKPTVLGLASGAVAGLATVTPAAGYISPLWAILIGLVAGMSCYA
                     AIVWKGRFGYDDSLDVVGIHGVGGVIGVLATGLCASKVVNPGGADGLFFGNPGLFGVQ
                     LLVVVVTTVFSLVGTFAILKLVDAMTGLRVSPEEEATGLDLSQHNERAYS"
     gene            complement(8133..9011)
                     /locus_tag="PLS35_08435"
     CDS             complement(8133..9011)
                     /locus_tag="PLS35_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18727.1"
                     /translation="MNMLVGRKARAWVLGLALMAGGGGGALVEAQAATTDDLMDTTKH
                     PVSVTMAMQADSFFGFNPSIYGTYGLTDNIALAFNFTYWTMISGVGVHDNAPWLETDV
                     GLNFTFLEKRLSVTPMIGFVHGSLLSSRGGFFPNGGGSVNERTTAFEGAVPNIIANYI
                     DNRFEGEFYMGYYKAIRSEGGSGGGGAIGCGNPGGTNCLGPIQGGGRGTWDFLHYWVS
                     AGVRVNSMWSMGAHYEHLLTTRDSSAPGAAQDYYRWIGPYVEMKLPGNMAFRFTVGKD
                     LTDNQDFYKLKFTKTF"
     gene            complement(9146..10918)
                     /locus_tag="PLS35_08440"
     CDS             complement(9146..10918)
                     /locus_tag="PLS35_08440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNI18728.1"
                     /translation="MMRVLRVAMAQINPTVGDIVGNTALIKTWINDARRAKADIVAFP
                     ELAITGYPPEDLLFKPRFIEDTQRALKAVAAEAHGLVVVVGYVGREATGVTPSEADAV
                     SPAGRHDLYNAAAVLCERRLVANYCKRLLPNYGVFDESRYFRPGTRLPLLVLNGTTIG
                     VNICEDIWFPEGPTRAQAAAGAEVIVNINASPFHVGKSRLREQVLATRARENRVMVTY
                     TNTVGGQDELVFDGRSVIVDHTGEVVARGKSFEQDLLIADLDVAAVGRARQGERRARP
                     LPPRVAALVDRVAVRVPARKSSSIAVPAMESPLDRLEEAYRALVLGVRDYVQKNGFKR
                     VVIGLSGGIDSALTAVIAVDAVGADNVLGVFMPSPYTSRASREDVADLAQRLRIQVDT
                     LSITTTFKSYLRALSKPFSGHRPDTTEENLQARIRGNLLMAYSNKFGHLVLTTGNKSE
                     MSVGYATLYGDMAGGFAVIKDVPKTMVYELSQLRNLNGLEPVIPKRVLERPPTAELRP
                     DQKDEDSLPPYSVLDPILKAYVEEDRALEDIVAMGFERETVARVMIMVDRSEYKRRQA
                     PLGIKITHRAFGKDRRMPITNGYR"
     gene            11263..12195
                     /locus_tag="PLS35_08445"
     CDS             11263..12195
                     /locus_tag="PLS35_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18729.1"
                     /translation="MLQSRPMHRVNYQRISRACEPMRLRKSWLLVLTCLLVVACSATS
                     PLIPVGEGPLGTVSLERLASRGTTARYGSPQSAFQASHPASVSASVISRLLSGLSVSG
                     VERPGAALRQERYPLFTQEEMEFFTPLIVKALSQAGPDQRVRFTLQDDGLITQGTLYL
                     HKTILRVSLSHYRASQGQSDTRPSALTLSFTPSEALVRDDVPQSWMIIEPEQPRIAVS
                     LEVLNELRAAVSAPTANKPVAVIPVAPAPAASEQTNLQQELQATKDVVVKQAQELQQL
                     KTELESLRRELAERDSTTPKAKPKAAPRKTVPTP"
     gene            complement(12470..12844)
                     /locus_tag="PLS35_08450"
     CDS             complement(12470..12844)
                     /locus_tag="PLS35_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18730.1"
                     /translation="MKAMRKAWCIACLVTVAACTTGQYQPLNGADKDEVEQLRENVYR
                     VEYRVSSFTSQEQLDSYLRRRCAELTLREGYNFFHLAQRADVLALSRRTSMTVTMYKG
                     KKPSGADDVYDAKEVLAGPLAQ"
     gene            complement(13306..14565)
                     /locus_tag="PLS35_08455"
     CDS             complement(13306..14565)
                     /locus_tag="PLS35_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HNI18731.1"
                     /translation="MASKRTVPQLEQALREKTREVDVLHRISESISSTLDLESVLRHI
                     VDVVVEATKADACLLYLLSDNRDELILRASKNPHPKLIGRITIGLGEGITGWVARERT
                     RVVIPNSASDDSRFKFFHNLPEDRYQAFVSVPITTKQEVVGVINVQHKRSRRYRPDEL
                     ALLSTIATQVGGAIENARLYDQMTRKALQLDTLSQVSETVSSNRLMEDVLQLIVTMTA
                     QMMGSKICSIILLDQPTGELRIAATQSLSEQYRRKPNVKIGQSISGRAVQDRRPIIVP
                     DVTKEGSYMYPDMAAKEGLCSLLCVPMLVREKAIGVINTYTSKPHTFTSEDVKLMQAI
                     ANQAAISIEHTTLLEKSFEMQEALQVRKLLDRAKGYLMRSKRLSEEDAFKLIQRQSMD
                     LRKSMREIAEAILLAGEIEDRADKNRG"
     gene            complement(14573..15730)
                     /gene="aroB"
                     /locus_tag="PLS35_08460"
     CDS             complement(14573..15730)
                     /gene="aroB"
                     /locus_tag="PLS35_08460"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNI18732.1"
                     /translation="MATSTPDMPRDTIHLELGARSYDIQIGRGLLKEIGVEMTRLRCA
                     GKVGVVTDRNLAGHYLKPVTRVLKAAGYTVVPIVLPPGERTKTLRSIGTVMDALVNAR
                     FERSSTLLALGGGVIGDITGFAAAIYQRGIPFVQVPTSLVAQVDSSVGGKTGVDHPKG
                     KNLIGAFNQPRAVLIDPATLRTLPEREWIAGLAEVIKYGVIEDESFFKYLEEHIAPIL
                     MLSDAPVAHIVKRSCEIKAQVVSEDEREADRRRILNFGHTIGHALESLGGYRGLIHGE
                     AVAVGMVYEADLARHLGYCHEDVVTRLRALVAAAGLPVRVPKVPFRALWDAMQQDKKV
                     SAGTVYCVVPERIGSVRIVPLAKEQTRAWYDTVSSDNTRSRQFIPNNKRRR"
     gene            complement(16000..17949)
                     /gene="pilQ"
                     /locus_tag="PLS35_08465"
     CDS             complement(16000..17949)
                     /gene="pilQ"
                     /locus_tag="PLS35_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNI18733.1"
                     /translation="MKQTQVGVHPLLSVTVMAGLLGLAGYVQAAAPGETTGLAGFTQA
                     TDQATSAEFTAAKEEHSTGATPTATLLTSVEAKPEGGRVALILTGNGVFDHTVKMVGD
                     RRMVLDMPHIQSQGRQTQLAVGHALLSKVRFGYHADKVRLVLDLAQPAEHSVTSVDGR
                     LVLMLEPKSVPTNDVKLAESEASPSAEQTAARSMVVSHKTPHALFRVQPIQLTAESVQ
                     KEDRKPETNEVVNGSSRFVGRRISLDFQQADISNVLRLIAEVSGFNIVVGEGVKNKVT
                     MKLANVPWDQALDMLLKMNALGMIRQGNIVWVDTLQNIAKQQDEEARAKDSKAKAEPI
                     VTRVFYIRNLNATEVQTSLRQNLSPRGTMTVSAASNALIISDTESKLEVLRQLLDGVD
                     LEVPQVQIEARIVQADTTYTRSLGVQWGIQNVNQLGASSGTSAFKTGTTGAFGAQVSD
                     FLINLPANPGLPSVPGAGFSIGKTDGAMLDVRLSAGELLGLTKVIAAPKITTLDKRDA
                     KIAQGESIPFQTTSLQGTQTTFVDANLELNVTPQITSRDPKEIGKQILMKVRATRNAV
                     GARSNPAGPSIDRREATTQVLVRDGETMVIGGVFVDTQSNNVAGIPYLSRVPVLGWLF
                     KNKTENVSKQELLIFLTPTIVRSAT"
     gene            complement(17946..18545)
                     /locus_tag="PLS35_08470"
     CDS             complement(17946..18545)
                     /locus_tag="PLS35_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNI18734.1"
                     /translation="MNTRTCWKMSIGAAVLVIGGTAMPVESKSLPHLRQIASMRPADS
                     LKVMPLPEGQKPAPAMESPAPRVEASVSQPGDSLSMEFSGTSYDPSGRRDPFLPMVQL
                     GQQVEQDASLPPLQRVGLTELSLIGVLWGNYGYTAMVQTPDGKGYSIRRGTRIGPNNG
                     VVSSITERGIIVQERFTDVYGNKQEREYVKLLHPKEGTE"
     gene            complement(18587..>19050)
                     /gene="pilO"
                     /locus_tag="PLS35_08475"
     CDS             complement(18587..>19050)
                     /gene="pilO"
                     /locus_tag="PLS35_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNI18735.1"
                     /translation="QLDTEIQNLTLKVKHLDELVAANKQLEIELAAKKERLPPEEEAV
                     MLLKQVSDLGLRLGLDVKLWKPGTQAEDPSKLFIRMPINVEVAGGYHTAAIFFDRISK
                     LSRIVTVQDVRIGAARVDQGRVVTQTVFDLVAYAAPQEKKPAAPVPAAKPK"
BASE COUNT         3982 a         6084 c         5120 g         3864 t
ORIGIN      
        1 gcacatgcgc gatcgtcagg tgacggagtt gcacttgttg ccgcgtggtc acatccatca
       61 cgccattgcc atacgccaac gcgatatcgg ccagcgcttg aagttgaacc gatgtggttt
      121 gccctcccgg catccgtact cgcaacatga agaagcccgg cgtgggattc cgcagaaaca
      181 gcccgtacca tttcagccgt tgaatatcgt cttcgggaat ggactcccac cccgcgtgcg
      241 catagtgctc aagcatgtcg cgtaccgcta atccgtcacg ctcggttttg atggcttcta
      301 tcttgttcat atcgtgctcc ccagtgcgac ggaatagtcc aatcccgatt ttctcaacac
      361 gacagtaccc tctcgactca atcgatccac gacgagcaag acctgcgagg cactcagacc
      421 ggagagggcc atcaattgct cgaatgattg gggagcctgc cgccggagaa gcgtcagcac
      481 tacggtttct tcattcgtct tcatgatgtc ctccttcgtc tgagtgccgc gtcacggcat
      541 gttggtcact gacttagaac gtcttatact cgagccactt gccgttcagc gtaatcttgc
      601 accgggcttc tccctggctg ccggtgactt tttccatatc cagcgtaaaa tcgatcgcac
      661 tcatgatgcc gtcgccgaac atttcattgg ccatctcacg cagcgaatcg ccgtagacac
      721 cgatgacctc catcaggcga tatttgaacg gatccgtcag gttcgggaaa tccgcgcgta
      781 tcggaaaggc gctcaaggcc gcgacctgtt cctgattcag acccaacagc gccccgacct
      841 tcgcggcgtc ggccggaacc aggcgatgat tgccgttcag ggctgccgcc acaaaggtcg
      901 gatttttgcc gacctttttc gccacttggg caatggtcac tttctttttt aatcgttgcg
      961 ccttgatcgc cttccgcatt gcttccttct ccatcatctc ctcctgtgat tgatccgcct
     1021 gcaatgatgc atactcgccg ctctaactta cgacccgacc accgccgatg tggcgggact
     1081 cgataccgga gtcaccggga cttcggccac ccggtaggga ctcaccatcc agtacaaccc
     1141 gccgacaaac aatccaccac ccacgaggtt ccccagcacg acaaagagtt ggttgtacca
     1201 aaaggccgcc cacgtcacgg tgacgtcatg cggtaggaac agcgccatac ccaacaagga
     1261 ttgattcgcg atgctatgct cgaaccccgt gccgataaag gcaaacaggc accagaagat
     1321 gagcaggatc ttggcggtgt cattcgtcgt ccgcccggtc atccatacgg ccaggcagat
     1381 cagccaattg caaagaatcc cccgcacgaa caactcccac gcgggaagag acattttcgc
     1441 cgccgccact ttttcaatca ggtccgccga cggccccttc gcgaacacgc cggattcgac
     1501 aatcaaccag gccaacccca tcgatccggc caaatttccg aaccaggacc aggcattgag
     1561 ctgaatgact tgcgtccagg ataggctgcg tgacagaccg ccgatggccc cgatcaagtt
     1621 gttgccggta aacagctcgg atcccgcaaa aatcacaagc gtcaatgcga taccgaatga
     1681 cacacccatg actagtttca ccaccggtga tccggcggcc gccaacggcc ctccgagact
     1741 gaaaatcaac gcaatgccga acccgagata aatcccggcc aaggacgaca ggatgaaata
     1801 cccgcccggc gcttgttcca aaaacatcca cttctttgcc gccacccccg cgatccgctc
     1861 atgatccgct gtgtgcatga ggctctccct tcgatggtcc atcagtctga acagctcaaa
     1921 aaaaaagacg ccctcatccc ctgtggggga gaaggacgtc tttgtcctgt tcaacgaggg
     1981 tgcgattccc tcgtcagtta tgctgcgccg acttcaacgt cggcagaaac gaaaaaggcg
     2041 tccgccactc ttgaaagaat gacggacgcc attgtccgtg taccgtatgt ggtgcaggtt
     2101 cgaaatcagg cgtcgttgcc tgctctcacc cacgcactgc atgtatacca tcgcctcccg
     2161 gtttcgtcaa gtgctctcga cgaggaggcc gccggctaca cgtcgtaata cagaaagaac
     2221 tcgtacggat gcggccgtaa ccgcatggta tcgacttcct tggtccgctt gtacccgatc
     2281 caggcctcga tgaggtcctc gctgaacacg ccccccttga ggaggaattg atggtccttc
     2341 tcgaggtggt tcagcgcctc gtccaggctg cccggcatgg tgcgaatttt cgccgcttcc
     2401 ttggcctcga gatcgtacag atccttttcc gccggctctc ccggattgat cttgttctcg
     2461 ataccatcca gaccggccat cagcatggcc gcaaaggcca gataggggtt ggccgcgggg
     2521 tccggaaagc gcacttcaat ccgcttcgcc ttggggctcg gggaatacat cggaatacga
     2581 ataccggccg aacgattgcg gctcgaatac gccaacaaga ccggcgcttc aaagcccggg
     2641 gtcaaccgct tgtacgagtt ggtcgacggg ttggtgaaag ctgccagtgc cggcgcatgc
     2701 ttcagaatac cgccgatgta atgcagacac atctgcgaca cgccggcata ttccttcccg
     2761 gcaaagagag gcttgccgtc cttccagatg ctctggtgcg tatgcatgcc cgacccgttg
     2821 tcgccgaaaa tcggcttggg catgaaggtc accgtcttcc catggcgacg cgcgacgttc
     2881 ttgacgatgt acttgaacat catcattttg tctgccgtcc gcaacaggct atcgaagcga
     2941 atatcgattt ccgcctggcc cgccgtcgcc acttcgtggt gatgcttctc cgtcgcgata
     3001 ccggccttct ccatctccaa aatcatttcc gtgcggatgt cctgctgggt atccgtcggc
     3061 ggaacaggga aatacccttc cttgtgacgg atcttaccgc ccaggttgac cccgtcctgg
     3121 cccatgttcc atacgccttc atcggaatcg atgtggtagt aggcgctgtg gttcgtctgg
     3181 tcgtagcggg catggtcgaa aataaagaac tcggcttccg gcccccagta ggaggtgtcg
     3241 ccgatcttgg tgctctggag atatttttcc gccttctggg cgatgaaccg aggatcgcgg
     3301 tcatacattt cgcgcgtgat cggatcgacc acattcccga tgaggctcag tgtgggcacg
     3361 gagcaaaagg gatccatgca ggcggttgcc gggtccggca cgagcagcat gtcgctgttg
     3421 ttgatcgctc tccatccgcg aatcgatgaa ccatcgagcc cggatccgtc cttgaacagg
     3481 ccttcggtca actcactgac aggaatcgta aaatgctgcc aggtgccgat caggtcgacg
     3541 aacttcatgt ccacgacctg gaccttattc ttctttgcaa aatctaacac ctcacgaacg
     3601 ttcatcccct cctcctttca accgccgata aaagaactac ccacaccctg acgtttacgc
     3661 cgcatgagct cccaaaaaca attcaatctc gttgagaatc gacgcccgaa tcgtttcgag
     3721 acgcccggcg tcttccacct tcttcccatc catccccgtc acgtaaaaca cgtcggccac
     3781 ttgatcgagc cgggtggaaa tccgggacgc atgcaccgac aagcccaatt gaaaaatagc
     3841 gttcgtgata atgtacagca atccttgccg atcatccgca aaaacatcaa gaatcgtaaa
     3901 ccggtcggac gtctcattat cgatacgcac ttccgccggt tgacgatggg cgggcaatga
     3961 ccggcccaga ttcaatcgcg ccccgcgccg catcaccgca tcgatcgact gacggccggt
     4021 cagcacctcc gtaatggtcg cgccgatcga ctcgcaccga tccgccggcg gagcgccctg
     4081 ataatcagga tcggcgacct ggaaggtatc gacgaccacg ccgtcttttc tggtcacgat
     4141 ctgcgcgtcc agaatctgca agccgccagc cgccatcacc ccggcaatct tcgaaaacaa
     4201 tccgggaatc agatcattat gtgcaatcac ggcatattcg catgttccca acggagcatt
     4261 gaactccgtc tccacgacca cgccgccctc ctgcagacgg cggatggcgc ccaagtgggc
     4321 ggcaatgcgc agcggactcg taccgtagac ataccgcagg ggaaactgtt cgagttcgga
     4381 acagatccag gttttgtcgg ctggaaggtt gtgatccccc tgcgactgtc ccgccacgtc
     4441 atcggcgata cgcgccaagc gctgcggctc atcggccgtc tcccgctcgc ccgatacttc
     4501 ctgcatggct cgcaggtaca gttcaaccag cagcgattct ttccatttcg tcagcacatc
     4561 agggcccacg gcagcgatgt cggcagcggt cagggccagt aacttgcgca acacttccgg
     4621 cgtgccgact tcacgcgcaa acggcaacag caccttttca tcatacgggt cgcggcgaaa
     4681 ggccgtgtga gccatcaaca gatgccgatg gaccagcatc accaaggtac gcgtgtccgg
     4741 ctcatcgaag ccgagacgcg cggccgtttc ttccgccaac cgctttccaa cctcactatg
     4801 atcttcctcg tgaccttttc ccaggtcatg aaggaggagc gccagatgca gcagatcctt
     4861 ccgtttgatc tctcgataga cctcaccgag gaccccgcta tggtcggcca gagtttcggc
     4921 tttcgccacc gcgagtaaac tatgctcgtc gaccgtgtac ttgtggtact gattgaattg
     4981 catcagtcca cgcacacgtg agaacgccgg cacgagtttc tccaacagat gcgcccgatg
     5041 catggcttcc agggtccgcg ccgtgcctgg gcccgccaga atcgaaagga aggtttggcc
     5101 tgcctccggc gtgtggaact gttcgacgga cagcgtctcc gcatgccggt gaatgtcttc
     5161 caggagagac ggatcgatac tcaactgccg cgaacgagcc aggttgaaga gcgtaagcag
     5221 caatgtcggc tgcaccagga cctgtgaacg caactcaacc ggaacggtca acgcctcacc
     5281 atcgacggaa aaatattgtt ggatgcgcgg cgacgggagc catcgaacga gccgttgcca
     5341 caaggagcgg cgtcggcacc gttccacaaa tcgcatcaag gcaacgtgca gccccatcgt
     5401 atgccgatag tattgctgca tgaattgttc gacggcgagc aaatgcggac ggtcggtgaa
     5461 cccgagatgt ttcgccagcc agacctgttc gtcgaaggtg agaatttcct gcgccatgcc
     5521 ggctcgaata tggagaaagg agcggacccg caagagaaac tctctggctt ccttgatcga
     5581 gcgataatct gattgggaga gaatcccccg gtcagagagt tcgcgaatgg tgggcgcctg
     5641 aaatcgcgcg gaaccgatcc attgcaggag gtgcaggtcg cgcaagcccc ctttgctttt
     5701 cttgacgttc ggctccagca ggtacaccgt ctccccgaac ttctggtatt cacgctgccg
     5761 ttcggcgacc ttctgttcga gatacccgtc gaaaccgctt gaaaccactt tgcgcacgta
     5821 gcgcgcatgg aattgttgaa agagctcggg gcttcccgtc aaaaaccggg cctccatcat
     5881 ggaggtgcgc acagtcagat ccgctaatcc caaatcgata cagtcctgaa tggtgcggac
     5941 gctgtgcccg acctgaaatc cgatatccca gagcggatgc agcacctgct tcaccagttc
     6001 aggaatgacc ttggccgcat cctgatgaaa gaggaacatc aaatcgatat cggagtgcgg
     6061 cgccagttcc cgccggccgt agcccccgat cgccaccagg cagcagtgct ggaatcctgc
     6121 ggtcgtgagc gcctcgccgc cggtcctggc cgcggtccgg taccggccga tgatcaagcc
     6181 atcgaccaga tccgttgtgg ccgcaaccac ttcgtcgcca gacgcaccgg ccagtaaccg
     6241 ctgctgaatc gcctggcgct gttcagccag cagttgagac accggaatgg cagctgcgtc
     6301 ctgcaccata tgaaaccccg cgtccaacgc gtgcgacata ctcagagcgc gctttctccc
     6361 gtttcaccgg tccggatcct gacggccgac gcgaggtccc gcacaaatat tttcccgtcg
     6421 ccgatgctgc ccgtctttgc ggcgcgcgtg atggtctcca acacccgctg gacctgacca
     6481 tcattcaccg ccacttcgat cttcaccttc ggcacgaact cgatggtgta ctcctggccg
     6541 cgataggttt ccttgtgccc cttctgccgg ccaaagccct tgacctctgt gacggtcatg
     6601 ccctgaatcc cgatctcgag cagggcatcc ttcacttcat cgagcttgaa cggtttgacg
     6661 atggcttcga ttaatttcat cctggcctcc tgagaccgcc gaccgcccac agtcgatggc
     6721 gtcgtatcac gaataggcgc gttcgttatg ttgactcaaa tcgagaccgg tcgcttcctc
     6781 ttcgggcgag acccgcagtc ccgtcatcgc atcgaccaat ttgaggatgg caaaggtacc
     6841 gaccagtgag aagaccgtcg tgaccaccac gaccaggagt tggacgccga acaacccggg
     6901 attgccgaag aacagtccgt cggcgcctcc gggattgacg actttgctgg cgcagagacc
     6961 tgtcgccagc actccgatca cccccccgac tccgtggatc cccacgacgt caagcgagtc
     7021 atcatagccg aatcgcccct tccagacaat cgccgcgtag caggacatcc cggcaaccag
     7081 gccgatgagg atggcccaca gggggctgat atatcccgct gcaggcgtga ctgtagccag
     7141 gccggccacc gcgccgctgg ccaatcccag caccgtgggt ttcccgcgat gcatccattc
     7201 cgcgccgcac caggagatgg ctcccataca ggccgccgca tgtgacgcaa tgatggccga
     7261 caccgccacg ccattcgccc ccagtgcgct ccccccgttg aacccaaacc agccgaacca
     7321 cagaaatccg gtcccgagca gggtcatcgg caaattgtgg ggagccatat aatccgtgcc
     7381 atagccccgc cgcttgccca gcacgatcgc acaaatcaat gcagccgcgc cggagctgat
     7441 gtggatgacc gcaccgccgg caaaatccaa cgcccccagt tgagcgagcc atccgccgcc
     7501 ccagatccaa tgggccagcg gcacatacac gagcaccgac cacatggcgg caaagagcac
     7561 aaccgaggta aaccgtttgc gttcggcaaa cgcgccggtg atcagcgccg gcgcaatggc
     7621 ggcaaacatc aattggaaca gcataaaggc ctggtgcgga atcgtcgggg catagaccgc
     7681 gtgcggctga ctccccactc cgctcaggcc gatccaatcc agccccccga tgaccccgcc
     7741 tttatccggt ccgaaggcca ggctatatcc gaagaggatc cacaacaggc tgaccacgct
     7801 caggatcatg atgctctgca tgatcgtccc cagcacattc ttggtgcgca ccagcccgcc
     7861 gtaaaacaag gcgagccccg gcatcagcat cgccagcacg aaggccgacg agaccaaaac
     7921 ccatgccgtg tccccgctgt cggctttgag cggcgcattc tccgcccacg cctgttccgc
     7981 ccccaatcca ccaatcaggg tgagtgcccc tgccaggccc acgaggaacg ttttccactg
     8041 tgccgatgcc ttcacgctgt cctccttcgg ctacgttgcc tgccaagagg cctcctcctt
     8101 caccatgctc aggaggaggc ccgggcagaa gcttagaacg tcttcgtgaa cttcagctta
     8161 taaaaatcct gattgtctgt cagatctttt ccgaccgtga accggaaggc catgtttccc
     8221 ggcaacttca tttcaacgta cgggccgatc caacgatagt aatcctgagc cgcccccggc
     8281 gcgctgctat cgcgcgtcgt cagcaaatgc tcatagtgcg cgcccatcga ccacatgctg
     8341 ttcacccgca cgcccgcgct cacccagtag tgcagaaagt cccaggttcc ccggccaccg
     8401 ccctgaatcg ggcccagaca gttcgtgccc ccgggattgc cgcagccgat cgctccgccg
     8461 ccgccgctgc cgccctcgct gcggatcgcc ttgtagtagc ccatgtagaa ctcgccttcg
     8521 aaacgattgt cgatgtagtt cgcaatgatg ttcggcaccg cgccttcaaa ggccgtggtg
     8581 cgctcattga cgctgccgcc gccgttcgga aagaacccgc cacgggacga caacaggctg
     8641 ccatgcacaa agccgatcat cggcgtgaca gacaagcgtt tttccaggaa cgtgaaattg
     8701 agtcccacgt ccgtttcaag ccagggcgcg ttgtcatgca ccccgacgcc gctgatcatg
     8761 gtccaatagg tgaagttaaa ggcgagggcg atgttatcgg tcagaccgta ggtgccgtag
     8821 atggatggat tgaatccgaa gaagctgtcg gcctgcatcg ccatcgtcac cgacacgggg
     8881 tgtttggtgg tatccatcaa gtcgtccgtc gtcgcggcct gcgcctcgac cagcgcgcct
     8941 cccccgccgc ctgccatcaa tgccaatccc aacacccatg cccgtgcctt tcgcccaacc
     9001 aacatattca tgacgcctcc aaagagagaa gaagtgaaag tggcccgctc cgagaccatc
     9061 gtgcccgaca atctggagtt ccgtctctct ggcgccattg ccagtgttgc tgaccgacgt
     9121 cgttgtcggt cgggatgctg cttgtctacc gatacccgtt ggtgatcggc atccgccgat
     9181 ccttgccgaa cgccctatgc gtaatcttga tgccgagcgg cgcttgcctg cgtttgtact
     9241 cgctgcgatc caccattatc atgacgcgtg ccaccgtctc ccgctcgaac cccatggcca
     9301 cgatgtcttc gagggcccgg tcctcctcga cataggcttt cagaatgggg tccaacaccg
     9361 aatagggcgg gagactgtct tcatcttttt gatcggggcg caactctgcg gtcggaggcc
     9421 gttccaggac acgttttgga atgaccggtt ccagaccgtt cagattccgc agttgtgaca
     9481 gttcgtacac catggtcttg ggcacgtctt tgatcaccgc aaatccgccg gccatgtcgc
     9541 cgtataacgt ggcgtagccg acgctcattt cgctcttgtt tccggtggtc aacaccaggt
     9601 gtccgaattt gttggagtag gccatgagca gattgcctct gatcctggcc tgcaagtttt
     9661 cctcagttgt atcgggacgg tgtccggaga atggcttgga cagcgcacgc aaataactct
     9721 tgaacgtcgt cgtgatcgag agagtatcca cctggatgcg cagacgttgg gcgagatcgg
     9781 ccacatcctc tcgactggct cgcgaggtgt aaggcgaggg catgaagacc ccgaggacat
     9841 tatccgcgcc gaccgcatcg accgcgatca cggccgtgag ggcggaatca atacccccgc
     9901 tcagcccgat gacgacccgc ttgaacccgt tcttttggac ataatcccgc acgcccagga
     9961 ccagcgcgcg ataggcctcc tccagccgat ccaatggcga ttccatggcc ggcacggcga
    10021 ttgacgacga tttcctcgcc ggcacccgca ccgcaacacg atccaccagc gcggccactc
    10081 gcggcggcaa gggcctggcg cgccgttcac cctgtcgcgc gcgccccacc gccgccacat
    10141 cgagatccgc aatcaaaaga tcctgctcaa aggacttacc tcgcgccacc acctcgccgg
    10201 tgtgatcgac gatcacgcta cggccgtcga agaccaattc atcctgccca cccacggtgt
    10261 tggtataggt gaccatcacg cgattctcgc gggcacgggt agccagcacc tgttcgcgca
    10321 gtcggctctt gccgacatgg aacggcgaag cgttgatgtt cacgatgacc tcagcccctg
    10381 ccgccgcctg cgcgcgtgtc ggaccctcgg gaaaccagat gtcctcgcaa atgttcacgc
    10441 cgatggtggt gccgttcagc accagcagtg gcagacgcgt gccgggccgg aaataccggc
    10501 tctcatcaaa gacgccatag ttcggcaaca gccgcttgca atagttggcg accagccgtc
    10561 gctcacacag cacagctgcc gcattgtaga gatcatgccg gccggccggg gaaaccgcat
    10621 ctgcctccga cggtgtcacg ccggttgcct cgcgcccgac ataccccacc accaccacta
    10681 atccgtgcgc ttccgccgcc accgccttta acgcacgctg cgtgtcttcg atgaagcggg
    10741 gcttgaacaa taaatcctcc ggcgggtatc cggtgatggc caattcggga aacgccacaa
    10801 tgtcagcctt ggcccgacgc gcgtcattga tccaggtctt gatcagcgcc gtattcccga
    10861 cgatatcgcc aaccgtcgga ttgatctgag ccatggccac tcgcaggacg cgcatcatca
    10921 gctttcccct gcactcgcac tttgctcaaa tcggtcattc aacgaggccg caaggagctc
    10981 aggcaccgag gcgtactgtg agccgtacgt cgaggtggct gatcgactga gaacgaagtt
    11041 gagggccgat ttcagcatag tgctcaaaaa aaaagtcctc ggacccaccg tatctcggtg
    11101 gaccagagga cgtcgttgtc ctgcgccatc tctataaggg atagcctcct gagactccgt
    11161 tgtccctgga aaagctggcg atggtatagc accagccgga atcggctgtc aaccaatcca
    11221 ggcgaggttt gcctgaaatc cccgtagcct ggagacgggt ctatgttaca atcccgcccc
    11281 atgcaccgcg tgaactatca gaggatctca cgagcttgtg agccgatgcg cctgagaaaa
    11341 tcctggctgc tggttctcac ctgcttgctg gtggtcgcct gcagcgccac gtccccgctg
    11401 attcccgtcg gggaagggcc attaggaacg gtctcgcttg aacgcctggc cagccggggc
    11461 accacggcca gatacggcag tccccagagc gccttccagg cctcccatcc ggcctcggta
    11521 tcggcatcgg tgatctcacg cctcctctcc ggcctctccg tttcaggcgt ggagcggccg
    11581 ggtgccgccc tgagacaaga gcggtatccg ctgtttaccc aggaagaaat ggagtttttc
    11641 acgccgctca tcgtcaaggc tctgtcacag gccggacctg accaacgggt gcggttcacc
    11701 cttcaggatg acgggctgat cacccagggc acgctctatc tgcacaagac catccttcgg
    11761 gtcagtcttt ctcactatcg cgcgtcgcag gggcaaagcg acaccaggcc gtcagcgctc
    11821 accttgtcgt ttacgccatc agaagcactc gtgcgagacg atgtgccgca atcctggatg
    11881 attatcgaac cggaacaacc ccgtatcgcg gtgtcgctcg aggttctgaa tgagctccgc
    11941 gccgccgttt ccgctccgac cgcgaacaaa ccagtcgctg taataccagt tgctccggcc
    12001 ccggctgcat cggaacagac caacctgcaa caggagctgc aagccacgaa ggatgtggtc
    12061 gtcaagcagg cgcaggaact tcagcagctc aagacagaac tggaatcgtt gcgacgcgaa
    12121 ctcgccgaga gggactccac cacgccgaaa gcgaaaccga aagcagcccc ccgcaaaacg
    12181 gtcccgacgc cctgagctag gtgctgacga ttgttacaag tccgtgggct cttctcctgt
    12241 caataatgtc gaagcgcagg ccttgccggg cgaggcggtc ccgggtgaac gccatcgcgc
    12301 ccttggccag catcaatgcc atagtacgag caaaacggaa gccgcgggca tgggatggtg
    12361 acttcagaaa tcaatcgaga acgtcacccg agcaaccgag agcgtcacag agtgcagcgc
    12421 ccgcttcgct gagggagcgg tagacacacg ttcgacaata catctctccc tattgagcca
    12481 gcggaccagc caagacctcc ttcgcatcat agacatcatc tgcgcccgag ggcttcttac
    12541 ccttgtacat cgtcactgtc atagacgttc gccgcgaaag cgccaacacg tctgccctct
    12601 gcgcgagatg gaagaagttg tatccctcgc gcaacgtcag ttctgcgcag cgccgccgca
    12661 aatacgaatc gagctgctcc tgggaggtaa aggaactgac gcgatattct acgcggtaga
    12721 cattctccct gagctgctcg acctcatctt tgtcggctcc gttgagcggc tgatactgtc
    12781 cggtggtgca agcggctaca gtgacaagac atgctatgca ccacgcctta cgcattgcct
    12841 tcacggttat cctcctcagg cgggcaaaga atgaatcacc cgacaacata aaacatgctg
    12901 gaccgccggc ttccgaagcc aactgtactg agaaaccgca cgatccttat tacgccggga
    12961 tcggcactcc gcactgacat ctccgctcca ccctctgctg atgaagaaga gctccaggct
    13021 cgtgttcgac tgtgacaagc tggcaaatgc cgcactgtga acgccccatc gacaccggtt
    13081 gctcacgcgc cgactcctga ggtcgcactc gcctccatac ccgccaccac tgcacgacga
    13141 tctgcaacac atcacaaaag gccagatgaa acagaaggaa cgagaggtag aggagctgcg
    13201 aaaggaactg gaagacatca aacgtcagct gggcaacacc tcgacccaag gcggccagcc
    13261 accctcaagt aggccataac atccgaagat agtagaactg tccggtcatc cccggttttt
    13321 atcggcccgg tcttcgatct cgcccgccaa caaaatcgcc tcggcaatct cgcgcatgga
    13381 cttgcgaaga tccatgctct gtcgctgaat caacttgaac gcatcttctt ccgagagccg
    13441 tttcgaccgc atgagatagc ccttggcccg atccagtaat ttgcgaacct gcaaggcttc
    13501 ctgcatctcg aaggactttt ccaggagcgt cgtgtgctcg atggatatgg ctgcctgatt
    13561 ggcgatggcc tgcatgagtt tcacatcctc ggacgtgaag gtatggggct tggaggtata
    13621 cgtattaatg acaccgatgg ccttctcacg caccagcatg ggcacgcaaa ggagcgaaca
    13681 cagcccttct ttcgcggcca tgtccgggta catgtaggag ccttctttgg tgacatccgg
    13741 gacaatgatc ggtcggcgat cctgaaccgc ccgcccgctg atactttggc cgatcttcac
    13801 attgggcttg cgccggtact gctcgctcaa actttgcgtg gcagcaatgc gcagttcacc
    13861 ggtcggttga tccaggagga taatggaaca gattttcgag cccatcattt gcgccgtcat
    13921 cgtcacaatc agctgcaaca cgtcttccat taaacgattc gacgagaccg tttcggacac
    13981 ttgggacaag gtatcaagtt gcagcgcttt gcgggtcatc tgatcgtaga gccgggcatt
    14041 ctcgatggca ccgccgactt gcgtggcgat ggtcgacaac agcgccaact catcagggcg
    14101 ataccgccgc gaccgtttgt gctggacgtt gatgacgccc acgacctcct gcttggtcgt
    14161 aatgggtacg gagacaaacg cctgataacg atcttccggc aggttatgaa aaaacttgaa
    14221 ccgagaatca tcgctggcgc tattgggaat caccacgcga gttctttccc gcgcgaccca
    14281 gccggtgatc ccctccccca atccgatcgt gatacggcca atgagcttcg gatgaggatt
    14341 tttggaggct cgaaggatca gctcgtcccg gttatcggac aacaggtaca gcagacaggc
    14401 atcggctttg gtcgcttcaa ccaccacatc gacaatatgc cgaagaacgg actccagatc
    14461 gagcgtgcta ctgatcgatt cgctgatgcg gtggaggaca tcgacttcgc gtgttttttc
    14521 acgcaacgcc tgctccaatt gagggactgt tcgcttcgat gccattgcgc tcctaccgcc
    14581 gccgcttgtt gttcggaatg aattgccggg aacgggtgtt gtcactgctg accgtgtcat
    14641 accaggcgcg agtctgttcc ttcgctaacg gaacgatgcg gaccgatccg atccgttccg
    14701 ggaccacaca gtagacggtt ccggcagaca cctttttgtc ctgttgcatc gcatcccata
    14761 acgcgcggaa cggcaccttc ggaacgcgca ccggtagacc ggccgctgca accagggcac
    14821 gcaggcgggt cacgacatcc tcatgacaat agcccaggtg ccttgccaga tccgcttcat
    14881 acaccattcc gacggcgacc gcttcgccat gaatcaggcc acggtatcct ccaagagact
    14941 ccaatgcatg gccaatggta tgcccgaaat tcaagatacg acgccggtcg gcttcgcgct
    15001 catcctccga caccacctga gccttaattt cgcaggaacg cttcacgatg tgtgcgacgg
    15061 gtgcatcact cagcatcaaa atcggcgcga tatgctcttc gagatatttg aaaaacgact
    15121 catcctcaat gaccccgtat ttgatcacct ctgccaaccc ggcgatccat tcccgctcag
    15181 gcaacgtacg tagtgtggcg ggatcaatca ggacggctcg cggttgatta aatgcgccga
    15241 tcagattttt tcctttggga tgatccaccc ccgtttttcc gccgacgctc gaatcaacct
    15301 gcgcgaccag gctagtcggg acctgcacaa agggaatccc gcgctgatag atcgctgcag
    15361 cgaatcccgt gatatcgccg atgaccccac cccccaaggc cagcagcgtt gaactgcgtt
    15421 cgaagcgggc gttcaccaga gcatccatca ccgttccgat ggaccgcagg gttttagtgc
    15481 gttcacccgg cggcaacacg atgggaacta ctgtatatcc ggcagccttc agcacgcgtg
    15541 tcacgggctt caaataatgc ccggccagat tccgatcggt gacgacgccc accttcccgg
    15601 cgcagcggag tcgcgtcatt tccacgccga tctccttcag caggccacgc ccgatctgga
    15661 tgtcataact ccgcgcaccc aattcaagat ggatcgtgtc tcgaggcata tcaggagtgg
    15721 atgttgccat gaactgttat cccgtgactt ggagagagcc tcgtcgtcgg cctcgcctag
    15781 cgcgagcgcc tcaccacggg cactaaggtc cttgaattac ccggtcggca atggtgtggc
    15841 tcagaaccgg atggtgaact gtacgattca gagggagaag aagcccggaa gaagcgccat
    15901 cgagaacggg gaggagacac gttccatggg attcgccccc attgagacag cgtaccatca
    15961 tcaatggggg cgattattag cgtgtcgagc ggggacctgt caagttgcgg agcgcacgat
    16021 cgtcggagtc aggaagatca acagttcctg cttcgagacg ttctcggtct tgttcttgaa
    16081 cagccagccc aggaccggga cgcgggacag gtacgggata cccgcgacgt tgttcgattg
    16141 cgtatcgaca aacacgccgc cgatgaccat ggtttccccg tcgcgcacca gcacctgagt
    16201 ggtggcttcg cgacgatcga tgctcggacc agcggggttg cttcgcgcgc cgacggcatt
    16261 cctcgtggcc cgcactttca tcagaatttg cttgccgatt tctttcggat cacgggacgt
    16321 aatttgcggc gtcacgttca gttccaggtt ggcgtccaca aatgtggtct gagttccttg
    16381 caaggaggtc gtctggaacg ggatcgattc accttgcgca atcttcgcat cccgcttatc
    16441 caaggtcgta atcttgggcg cagcgatgac cttcgtcaac ccgagcaact caccggccga
    16501 caaccgcaca tccaacatcg caccatccgt tttgccgatc gagaatccgg cgccaggcac
    16561 agacggcaat cccgggttag ccggcaagtt gatcaggaag tccgacacct gcgcaccgaa
    16621 ggctcctgtg gtgccggtct tgaaagcgga ggttccgctc gaggcgccca actggttgac
    16681 gttctgaata ccccactgca cacccaacga ccgggtatac gtcgtgtcgg cttgaacgat
    16741 gcgcgcctcg atctgcacct gcggcacctc gagatccacg ccgtccagca actgccggag
    16801 gacttccagc ttggactccg tgtcgctgat gatcaaggca ttgctcgcgg cgctgacggt
    16861 catggtgccg cgtgggctca ggttctgccg caaggaggtt tgcacttccg tcgcgttgag
    16921 attgcggata tagaacactc tcgtgacgat gggctcagcc ttcgccttgg aatccttggc
    16981 gcgcgcctct tcgtcctgct gcttggcaat attctgcaga gtatccaccc acacgatgtt
    17041 gccttggcgg atcatgccca gagcgttcat cttcagcagc atgtcgaggg cctgatccca
    17101 gggtacattg gccaacttca tagtgacttt gttcttcaca ccctcgccga ccacgatatt
    17161 gaagccgctg acttcagcga tcaaacgcag cacattgctg atgtccgcct gctggaaatc
    17221 gagagaaatc cgccgcccga caaagcggga cgaaccgttt accacttcgt tcgtctccgg
    17281 cttccggtct tccttttgaa ccgactccgc cgtcaattgg atgggctgca cacggaacaa
    17341 cgcatggggc gtcttatggg agactaccat cgagcgcgct gcagtctgtt cggccgacgg
    17401 cgaggcctcg ctctcggcca gcttcacatc attggtgggc acagacttgg gttccagcat
    17461 gagaaccaac cggccgtcga ctgaggtgac gctgtgctcc gccggctggg ccaggtcaag
    17521 gaccaatcgc accttgtcag cgtggtagcc aaatcgcact ttcgacaaca acgcatgacc
    17581 gacggccaac tgcgtctggc gtccctgcga ttgaatgtgc ggcatatcga gcaccatgcg
    17641 gcgatcaccg accattttga ccgtgtggtc gaacaccccg ttaccggtca ggatcagcgc
    17701 cactcgtccg ccttcaggct tggcttcaac gctcgtcagc aatgttgcgg tcggagtggc
    17761 cccggtggag tgctcctcct tcgctgccgt gaactcagca gaagtcgcct gatcagtggc
    17821 ctgcgtgaat ccggccagtc cggtcgtttc ccccggtgcg gcggcttgca cgtagcccgc
    17881 aaggcctaac agtccggcca tcacagtgac actcagcagc ggatgtacac caacctgtgt
    17941 ttgtttcatt ctgtgccctc tttcgggtgc aggagcttga cgtattcgcg ctcctgttta
    18001 ttcccgtaca cgtccgtgaa ccgctcttga acgatgatgc ctcgttcggt gattgaactg
    18061 accacaccat tgttaggccc gattcgtgtt ccccgacgga tgctataccc cttgccatcc
    18121 ggcgtttgaa ccatggccgt gtacccgtag ttgccccaca gcacaccgat cagactcagt
    18181 tccgtgaggc cgacacgctg cagcggggga agactcgcat cttgctccac ctgctggccc
    18241 agctggacca tcggcaggaa ggggtctcga cgtcccgaag gatcgtagga ggtcccggaa
    18301 aactccatcg acagcgaatc acccggctgc gagaccgacg cctcgacacg aggagccggc
    18361 gattccattg caggagccgg cttctgcccc tccggcagcg gcatgacctt cagggagtcg
    18421 gcgggccgca tggaggcgat ctgccgcaag tgcggcaggg atttcgactc caccggcatg
    18481 gcagtaccgc caataaccag cactgcggct ccgatgctca ttttccagca agttcgtgtg
    18541 ttcacggtac gtcctcttac attggacatg cgctgggctc cattcctcac ttcggcttcg
    18601 ccgcaggcac cggcgcagcc ggcttcttct cctgcggagc ggcataggcg accagatcaa
    18661 agactgtttg cgtcaccaca cggccttggt cgacccgggc agcgccgatc cggacatcct
    18721 gaacggtcac gatacgggac aacttgctga ttcgatcgaa gaagatggcg gctgtatggt
    18781 acccgccggc cacttctacg ttgatcggca ttcgaatgaa caacttcgaa ggatcttcgg
    18841 cttgcgtgcc cggcttccac aacttcacat ccaggcccaa ccgcagtccc agatcggaca
    18901 cctgcttcag caacatgacg gcttcttctt ccggcgggag gcgttccttc ttcgccgcca
    18961 attcgatctc caactgctta ttggccgcga ccagctcatc caggtgtttg accttgagcg
    19021 tcaggttctg gatctcggta tccaactgag
//
LOCUS       DAOQEW010000060        18995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_740_length_18995_cov_8.863992, whole genome shotgun sequence.
ACCESSION   DAOQEW010000060 DAOQEW010000000
VERSION     DAOQEW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18995
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_18995_cov_8.863992"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLS35_08480"
     CDS             <1..376
                     /locus_tag="PLS35_08480"
                     /inference="COORDINATES: protein motif:HMM:NF039842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HNI18736.1"
                     /translation="ISIIDDHLSRSPIIPRVVFGQPFHFKRSRILEVPIGLEARTLQE
                     FRDIVTDIDVSSIYYHMFEAHFRLRRAENDFSAWLRTSLKLTNLADRIRSINPYQGSL
                     ERLRSKLISACDDFLTSESAKS"
     gene            380..2059
                     /gene="treS"
                     /locus_tag="PLS35_08485"
     CDS             380..2059
                     /gene="treS"
                     /locus_tag="PLS35_08485"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HNI18737.1"
                     /translation="MLNQDPLWYKDAVFYELHVRAFYDGNDDGVGDFPGLIQKLDYLE
                     WLGVDCLWLLPFYPSPLRDDGYDVADFRSIAASYGTTDDFRRLLEEAHRRGMRVIVDL
                     ILNHTSDQHAWFQAAKASPDAPTRNYYVWSETDQKYPRARIIFVDTEKSNWTWDHEAQ
                     AYFWHRFFSHQPDLNYDNPEVRRAMIQVMEYWLDQGLDGFRCDAVPYLFEREDTICEN
                     LPETHQYLKDIRSSIDQRYKGRVLLAEANQWPSDVRPYFGDGDEFHMAFHFPLMPRLF
                     MGVRSEDRQPIVDMFKHTPDIPSACQWCLFLRNHDELTLEMCTGEERDYMYYAYARDP
                     QMRRNIGIARRLAPLLDNDRRKVELLNSIVFTLPGSPIIYYGDEIGMGDNIHLGDRNG
                     VRTPMHWTADRNAGFSKADPSQLFLPVVADAVYGYQAVNIDAQKQATHSLLNWMRRMI
                     AIRKKHKVFGRGTLQFLSPANEKVLAYIRAYEQETLLLVHNLAGSSQAVELDLSRYKD
                     VVPEELLGESRFPTIHEHPYVLTLGPYSSYWLHLPAVRSPTTAYSMEWSAI"
     gene            2108..3307
                     /locus_tag="PLS35_08490"
     CDS             2108..3307
                     /locus_tag="PLS35_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18738.1"
                     /translation="MHSALDEYREVSPKGTVDFLYRLSDLVQGKSFLHLNAVRYGGGM
                     SEVLRRLVPMMVSLGVEARWEVLAGSQEYFVVVTRMLNALQGRDDKLTDDMYQTYANV
                     IERNAKALKLEADMVMVHDHQPAGLVDYRTNGCWLWRCHLDLAQPQRPAWAFLRRYVL
                     KYDAAIFSLSGFAQRLPIPKFLIYPSIDPLSPKNRDMSRSEIAQVLDRLAIPRVKPTI
                     LQVARFDRFKDPLGAIHTYQLVKRHYDCQLVLAGSGVMHDPEGEAVLMELREAGARDP
                     DIHVIQLPPEADLEINALQRSATVVLQKSVKEGFGLTVSEAMWKGKPVVGSTAGGIAA
                     QIVDGVTGYVVHSVEGAAFRIRHLLNNPGLMNRMGAMAREHVRRNFLITRQLGDYLTL
                     LKLLAAA"
     gene            3324..>4183
                     /locus_tag="PLS35_08495"
     CDS             3324..>4183
                     /locus_tag="PLS35_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18739.1"
                     /translation="MTTVMSKPQGGKSETEVLAPSPELSVSRTDMEWPTDTIDIMIGL
                     LTLNNAGSIEPVLKSIVEGMRQFFPHASALLVNCDAGSQDDTPGLITRLADGVIPVRI
                     VSNATGSYAHLTREPGFPGRDESIHNFCLTAQRLRPKICLILEGQLRSLSPSWIDHLG
                     RPVYEQGEDYVVPLYRRHRYEGTLATNLLYPLTRALYGKRLRYPSGGAYALSGKFAGD
                     VISQPTWKGPAAKYSVDSRLTTAALAGGYRVCHTCLGTRDQESTLGAVDLSVLIAQSV
                     GGIFHSMEAY"
     assembly_gap    4184..4283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4390..5091
                     /locus_tag="PLS35_08500"
     CDS             4390..5091
                     /locus_tag="PLS35_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18740.1"
                     /translation="MSDLWRETMVAGFRDTVMQIVLVLPRLLALLTFLSLGLLAAWSV
                     KVLTLRVLRTFGFDSFCERLGIVQPLSQAGVRRPVSLVVSGVLFWIVFLLFAFMGVDA
                     MNLPATANLISQIVAFLPNVLAASLVLLVGVLCANFFAEAALIAAVNAQVEEARVVAG
                     LVRWGLLLFTCAMVLTQLGIAKEIVIAAFSITFGGVVLALALAVGLGARHLMREILER
                     RFSQHEHKADQLSHL"
     gene            5124..7160
                     /locus_tag="PLS35_08505"
     CDS             5124..7160
                     /locus_tag="PLS35_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HNI18741.1"
                     /translation="MPSKPASRKKQASSPVLGEGRLRVAIEHVEPQADDGRYPIKRVV
                     GETIIVEADLFADGHDVIQGILRSRHERQEEWLTTPLSPLVNDRWRASFDVTELGQYR
                     YGIMGWVDPFATWQRDMRKRLRARQDVTVEILIGKHLIEQATAPAPDVSRRRLKDIQA
                     RVSRSDLASQALLDLLLEEDTAEIMADCIDHALMSTYDRELIVVVDRPKARFSAWYEM
                     FPRSTTDEEQPHGTFKDCESRLPYIAEMGFDVLYLPPIHPIGETHRKGRNNNPRGDSS
                     SVGSPWAIGSRDGGHTAIHSELGSLGDFEQLVAAAQTNGIEIALDLAFQCSPDHPYVK
                     EHPEWFKTRPDGTIQFAENPPKQYQDIFPLHFETDAYPDLWKELREVVQYWIDHGIRI
                     FRVDNPHTKPFLFWKWLISDVKASYPETIFLAEAFTRPKVMYHLAKIGFTQSYTYFAW
                     RNSKQELTDYFTELTTSSVREYFRPNLWTNTPDILTEHLQHGGRPVFMARLALAATLG
                     SSYGIYGPAFELYENRPQKPGSEEYLDSEKYEVRRWDINRPDSLKEFIGLINRIRREN
                     PALHRDESLQFHPIDNEYLLAYSKQSADGSNVILIVVNLSPHHVHSGWLELNLAALGI
                     EANQPFQVHDLLTQAYYVWQGPRNYVQLDPHSVPAQIFTVRRNLRREEDFDYYL"
     gene            7185..8900
                     /locus_tag="PLS35_08510"
     CDS             7185..8900
                     /locus_tag="PLS35_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HNI18742.1"
                     /translation="MITVRDNWKVAFEGSSRAEIEKLLPDFLTASRWFGGKAKTIRSA
                     KLADVLQQEHGDGTMVLALIDVSYLEGGADRYTLPMTAAFDAEADRVKHDYPEAIIGA
                     LTVLQNGTALAGILYDALWHPDCAYSLLDSMGRRGQFRRSIGTLVGSATELFDEASHC
                     AHAASSSVLKGEQSNTSVKFGDCAIMKLYRRIEPGMNPELEVGRALTARHFTHSPSLI
                     GALEYVQEAGEPMTLALAQTFVANRGNAWDYTLEQLSRYINGILALDHRKEEAGQPAH
                     DRSNQEPTTSDLLLGYRDSAHLLGQRTGELHIALAQPSDEAAFTPEPCATSYVQSRVH
                     AMQQSATQALALLRRRLPSLSVTDQEQAQKVLEQESPLLSRLATLARQPLSALRIRCH
                     GDYHLGQVLYTGEDFVIIDFEGEPAKPLAERRAKCLPMVDLAGMIRSFHYAAHVALRT
                     GEARRPHLPASLDLVSWMEHWYRTVRTAFLRGYRTTAGQAPFSPQSPDEFNLLLDVQM
                     LDKAMYELTYELNNRPDWVGIPLTGLLHSMSASIAIEDADNDDVRNDGAIPRSGGQVR
                     DEVNQ"
     gene            8897..10840
                     /gene="glgB"
                     /locus_tag="PLS35_08515"
     CDS             8897..10840
                     /gene="glgB"
                     /locus_tag="PLS35_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNI18743.1"
                     /translation="MSPQNLAQNPGPSATTRLTDDDVYLFNEGTQFQLYDKLGAHPMV
                     SHGEAGTYFAVWAPNAEQVSVIGSFNDWNRDAHILHPRGDSGLWEGFITECAKGALYK
                     YHVHSRHADYRVDKSDPLSFFNEIPPKSASIVWDLDYTWTDDDWLRTRGRANALNAPM
                     AIYEMHVGSWKRIAAEQNRSLSYRELAVPLAEYLQKTGFTHVEFMPLTDHPFFGSWGY
                     QTTGYFAPTGNYGTPQDLMYLIDTLHDHGIGVLLDWVPSHFPTDEQGLGFFDGTHLYE
                     HADPRQGFQLDWNTFIFNFGRNEVRSFLISSALFWLDKYHFDGLRLDAVASMLYLDYS
                     RKEGEWIPNRYGGRENLEAIAFLKRLNEEVYRHFPDVQTIAEESTAWPLVSRPTYLGG
                     LGFGLKWDMGWMHDTLTYMQKDPIDRKYHHHNLTFRMLYAFHENFVLPLSHDEVVYGK
                     GSLLGKMPGDEWQKFSNLRLLFGHMYSQAAKKLVFMGGEFGQPAEWSHDGQLEWSALE
                     LPLHQGVHRWVTDLNHTYRRESSLHEGDVDPSGIEWIDCRDAESSVISLIRKGRATDE
                     IILVVCNFTPVPRPNYRLGAPRGGYWKELLNSDAAIYGGHGWGNAGGIEATPVPLHGR
                     THSITCTLPALSVLFLKHSSSPL"
     gene            11124..11615
                     /locus_tag="PLS35_08520"
     CDS             11124..11615
                     /locus_tag="PLS35_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18744.1"
                     /translation="MKNTIALYSTAFLIGAWPLLDLSQAQAFSNNPEPVVWNSNQKSA
                     AWAEELLGQVVTYQTMAEKNLIPGSFQPYVEQTSKIRELHRSGNRRATYDGVNALMVM
                     LEARVGGIDEHSAEALWDFCYRVTPDEFHARDRHIRAKGADTVKEHEEFMRNMEERAG
                     MSF"
     gene            11748..13433
                     /locus_tag="PLS35_08525"
     CDS             11748..13433
                     /locus_tag="PLS35_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNI18745.1"
                     /translation="MWKFLLLWFAFTLPSGAILAPTSLMAEAGHVGYGPTSPALFSSA
                     ESLVIDTDPVALLPPPAAFDIRNFWMRADLRVRPEWRSGVCFGGGPPMNGACNSLNLS
                     GSGTSAHDGSKASDFFIQQWARLGLGYDPSPTLNFYVELQDSATWGGSGNPTDGGQSA
                     DAANHQCGTQRPGQCRLGIRAGYALIRNLAGVEGLSAKFGRQYVVFGNQRLFGHFDWA
                     NTGYSHDGVMLSYARTDFDLKLGWFRHSETDLGQGHPGGSLTPNLLTCNQDVTSQSVC
                     NPAVAQHAADAGSDVDMVVFYNQIRGVPRMIVEPYYVLYSNRLPERANPGQYLPKSAS
                     QLRHMVGLRLELRDGNWDLVHETAYQFGRTADGFGFDNQRNLSINAWASGTWLGHTWY
                     AHPWKPRVAIGFDYASGDGDSNCVTPGGTLARSCGGNANTFENFFPTNFLHVGYMLNG
                     AWRNTVQPQVNLQARPTTRDHVEVWAMRKYLASARDNWYRGSQGPLIFSRSDNQTTHV
                     GDELDVAWSHMFADGTVSVAVIYGHFFSGPYIRHQLGTSMDQDWGIVQLWTNF"
     gene            13440..14993
                     /locus_tag="PLS35_08530"
     CDS             13440..14993
                     /locus_tag="PLS35_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI18746.1"
                     /translation="MQRATLNFETLLEVTNALNSQRDIESLWRVIADQIQKVIPWDRA
                     GITLYESNTDAFRFYAVITNMASPALAHDSVIPREGSAVGWVYDHRRLHIRGNLQKEQ
                     VFLEDRFYAQEGLGRMINLPLLVREHCLGTLNIGSVHGGEPDPDDCKFLQQVATQIAY
                     AIDHVLAYQQIKQLSERLRRENEYLAEEVKASRNQRLLVGASSSFSKVVELVKAVAPT
                     DTTVLLLGETGTGKEVLAQALHDLSARSQKPFIRVNCAALPSGLIESELFGHERGAFT
                     GAQLRRAGRFELAHTGTLFLDEIGEMPLETQAKLLRVLQDGMVDRIGGTQPVSVNVRL
                     IAATNADLSAAIQQGTFRADLYYRLHIFPIAVPPLRERREDIQLLAQHFLTQIGAKHK
                     RPHLTFDPQSMARLLSYNWPGNVRELQNVIERAVILSSSSQVTVDEMLLPIVRAGRAV
                     LTHQPESLEDLERHHIIMILERTKWRIYGDQGAANLLGLNPETLRSRLRKLGIRRPVS
                     RSSETSPMA"
     gene            15354..15635
                     /locus_tag="PLS35_08535"
     CDS             15354..15635
                     /locus_tag="PLS35_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18747.1"
                     /translation="MKITSVWRGNKPNHKKKRTVPKLRIRWTELGLVDPSVEPHETPM
                     DQIRREVTALASSGFGAETRARSEQTQTRPARTLARTSTTRTARRPAGS"
     gene            complement(15915..16256)
                     /locus_tag="PLS35_08540"
     CDS             complement(15915..16256)
                     /locus_tag="PLS35_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18748.1"
                     /translation="MSDQNQDAPRAATQWVRIPDGTMVQHRLDGQKGHIDGLTEIVTG
                     PSRNPDGRTQYRINVGKGDRVLIAEEDLLILTDAEGMVRMAKEKGEYRSLVSKQLRGI
                     FGEDRFIVKTS"
     gene            complement(16292..17587)
                     /locus_tag="PLS35_08545"
     CDS             complement(16292..17587)
                     /locus_tag="PLS35_08545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI18749.1"
                     /translation="MQTTAFTSFDSLGLSPTLLRNLTKAGFSEPTAIQAQAIPHALSG
                     RDVLGCAQTGTGKTAAFVIPMLERLTGTPKGQPRALILAPTRELAIQIQATIDTLGRD
                     LQLFATTVVGGADMQAQVRGLRQRPDIIVATPGRLLDHMWNGTISLLAMNILVLDEAD
                     RMLDMGFAQQINQILDAMPEERQTLLFSATMPMDLARLAQASVKDPVRVMVAKSATTA
                     EGVAQAVHHTTHDRKNALLMSLLQSESDTVLVFARTKHRADRLGNLLDTAGHRVAVLH
                     GGRTLPQRRAALEGFRRGTYRVLVATDIAARGIDVANIAHVINYDVPNCPEDYVHRVG
                     RTARMRTTGRATTFVTAEDQEQLRAIERLLGQAVPRAEGSPASLPPSSRPDGHAPRNE
                     PERRRRRGGSAQGWRQTTDGGSREANGGIKNGSDLGPVS"
     gene            17880..18164
                     /locus_tag="PLS35_08550"
     CDS             17880..18164
                     /locus_tag="PLS35_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18750.1"
                     /translation="MRMILAALCLCVSTALCTVSSYAAENSGGGKNGSNQGMTLDDIG
                     RGLKSAAKNIEEEIPKVGPAISDTFKKVRDGRKEDSSAKASPQHSSKDKK"
     gene            18214..18597
                     /locus_tag="PLS35_08555"
     CDS             18214..18597
                     /locus_tag="PLS35_08555"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HNI18751.1"
                     /translation="MLHTTAGCLATQSPVCLPGERHAIMESIYFGTATPRGVVSPDEW
                     AAFVNDTVTPAFPEGLTSWQASGQWRKQNASIEHKDSHILQLIHEGNAVKNTAIQRLI
                     SIYKQRFEQEAVMRVRSGACLSFPQ"
     gene            complement(18624..>18995)
                     /locus_tag="PLS35_08560"
     CDS             complement(18624..>18995)
                     /locus_tag="PLS35_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18752.1"
                     /translation="LPDAVTRQIVGTQVGHALEFEKNLQRPSSDGAEREPYGSATKGT
                     ILATSIGLPLISASPTSGQASALGASTSGSTIISPPTGGALQAAGVGGTGPAQSGGLN
                     SSKLLQQILNDVGKIKKGKDR"
BASE COUNT         4126 a         5519 c         5269 g         3981 t
ORIGIN      
        1 gatttccatc atcgacgacc atctttcccg cagccccatc attcctaggg tggtgttcgg
       61 ccagccattc catttcaagc gctccaggat ccttgaagtc cccatcggtc tcgaggcgcg
      121 cacgctgcag gagttccgcg acatcgtgac ggacatcgat gtgagcagca tctactacca
      181 tatgtttgaa gcgcactttc gattacgacg ggccgaaaac gatttttcgg cctggctccg
      241 caccagtctg aaattgacga atctggctga ccgcatccgc tcgatcaatc cctaccaggg
      301 cagtctggag cggctgcgtt ccaaactcat cagcgcatgt gatgatttcc tgaccagcga
      361 gtcggcaaag agctgatcca tgttgaatca agatccgctt tggtacaaag acgccgtgtt
      421 ttatgaactc cacgtccgcg ccttctacga tggcaacgac gacggagtcg gggattttcc
      481 aggcttgatt cagaaactgg actatctcga atggctcggt gtggattgct tgtggctgct
      541 ccccttctat ccctcgccct tgcgggacga tggatacgat gtcgcggact ttcgcagcat
      601 cgccgcatcc tacggcacga cagacgattt tcgccgtttg ctggaggagg cgcaccgccg
      661 cggcatgcgg gtgatcgtag acctgattct caaccacacc tccgaccaac atgcctggtt
      721 tcaggcagcc aaagcttctc cggatgcccc cacccgcaac tattacgtct ggagcgagac
      781 ggatcagaaa tatcctcgcg cacggattat tttcgtcgat acggaaaaat ccaattggac
      841 ctgggaccat gaggcacagg cctacttttg gcaccgcttc ttcagccacc agccggacct
      901 gaactacgac aaccccgagg tgcgccgggc catgatccag gtcatggagt attggctcga
      961 tcagggcctc gacgggtttc gatgtgatgc ggtgccctac cttttcgagc gcgaagacac
     1021 catctgcgaa aatctgccgg aaacccatca gtacctgaaa gacatccgca gcagcatcga
     1081 tcagcgatac aaaggccggg tcttattggc cgaggccaat caatggccca gcgacgtgcg
     1141 cccctacttc ggggacggcg atgaattcca catggccttc cattttccgt tgatgccccg
     1201 cttgttcatg ggcgttcgga gcgaggatcg gcagccgatc gtcgacatgt tcaaacatac
     1261 gccggacatt ccatcagcct gccaatggtg cctctttcta cggaaccatg atgagctgac
     1321 attggaaatg tgtaccggcg aagagcgcga ctatatgtat tacgcctacg cccgcgatcc
     1381 gcagatgcgt cgcaatatcg ggattgcccg ccgcctggct ccgctgctgg ataacgatcg
     1441 ccgcaaggtc gagcttttga acagtatcgt cttcaccctt cccggcagtc ccatcattta
     1501 ttacggagat gaaatcggga tgggcgacaa cattcacctt ggcgacagga acggcgtgcg
     1561 caccccgatg cattggaccg ccgatcgtaa tgccggcttt tccaaggccg acccgtcgca
     1621 attgtttctt cctgtcgtcg cggatgccgt ctacggttat caggccgtca acatcgacgc
     1681 gcagaaacaa gccacccatt cgttacttaa ttggatgcgc cgcatgatcg cgatccggaa
     1741 gaagcataag gtgtttgggc gcggaacgtt gcagttcctc agtccggcca atgaaaaagt
     1801 cctcgcgtac attcgtgcct atgaacagga aaccttgctg ctcgtgcata acctggcggg
     1861 atcctcacag gccgttgagc tggatctcag tcgctacaaa gacgtggtgc ccgaagaatt
     1921 gctcggtgag tcacgcttcc ccaccattca cgagcacccg tatgtgctga ccctcgggcc
     1981 ctatagctcg tattggttgc acttgccggc cgtgcggtcg ccgacaaccg cctacagcat
     2041 ggaatggagc gcaatttgac gaacatgaat cgcccgggat ctcacgccac gtaaggagaa
     2101 ggatcgagtg cattcggcat tggacgaata tcgtgaggta tcgccaaaag gcacggtaga
     2161 ttttctctac cgtctgagcg acctcgtaca aggcaagagc ttcctgcacc tgaatgccgt
     2221 caggtatgga ggcgggatgt cagaggtgtt gcggcggctc gtgccgatga tggtgtcgtt
     2281 aggcgtcgag gcccgatggg aagtcctggc cggatcacaa gagtactttg tggtggtcac
     2341 gcgcatgctg aacgccttac agggccgaga cgacaagctg actgacgaca tgtaccagac
     2401 ctatgcgaat gtcatcgagc gaaatgccaa ggcgctgaaa ctcgaagccg acatggtgat
     2461 ggttcacgac catcaaccgg cgggactggt cgactatcgg accaatggct gctggttgtg
     2521 gcgctgccac ttggacctcg cccagcctca acgtccggcc tgggcgtttc ttcggcgtta
     2581 cgtcctcaaa tatgatgccg ccattttctc gctgtccgga tttgcccaac gactaccgat
     2641 cccaaagttt ctcatttatc cgtccatcga tccgctgagt ccgaaaaacc gtgacatgag
     2701 ccgatcggag atcgcgcagg tgctggatcg actcgccatt ccccgggtga aaccaacgat
     2761 cttgcaggtg gcacgatttg accgcttcaa ggatccgttg ggtgcgattc acacctacca
     2821 actcgtcaag cgtcattatg attgccagct tgtgttggcg gggtccgggg tcatgcacga
     2881 tccggagggc gaagcggtcc tcatggagct ccgggaggcc ggagcgcgcg atcccgacat
     2941 tcatgtcatt caacttcccc cggaagcgga tctggagatc aatgcgctgc agcggagtgc
     3001 cacggtggtc ctgcaaaaat ccgtcaaaga agggttcggg ttgaccgtgt cggaagcgat
     3061 gtggaaaggc aaaccggtcg ttggcagcac cgccggcggc attgccgccc agatcgtgga
     3121 cggagtgacg gggtatgtgg tccattccgt agagggcgcg gcattccgta tccggcattt
     3181 gctcaacaat cccggcctga tgaacaggat gggggccatg gcgcgagaac atgtacggcg
     3241 caatttcctg attacccggc agctgggtga ttacctcacc ctgctcaaac ttctggcggc
     3301 agcgtgagag ggatggagac ttcatgacca ccgtcatgtc gaagccgcaa ggcgggaaga
     3361 gtgagacgga ggtgctggca cccagcccag agctgagcgt gtcgagaacc gacatggagt
     3421 ggccgacgga cactattgac atcatgatcg gcctcttgac cctcaacaat gccggatcca
     3481 tcgagcctgt cctgaaatcg attgtggaag ggatgcggca attcttcccc catgcgtctg
     3541 cgctgctcgt gaattgcgat gcgggttcac aagacgacac tccaggcctc atcacccggc
     3601 tggcagacgg cgtgattccg gtccgcatcg tgtccaatgc gactgggagc tacgcccatc
     3661 tcacgagaga acctggcttt ccagggcgag acgaaagcat ccataacttc tgcctgacag
     3721 cccagcgcct ccgaccaaag atatgcctga ttctggaggg acagctgcgc tcgctgagcc
     3781 caagctggat cgaccatctc ggcaggccgg tctacgaaca gggcgaagac tatgtggttc
     3841 cactatatcg tcgccatcgg tatgagggga cactcgcgac caatctgctg taccccctga
     3901 cccgtgcgct gtacggcaaa cgattacggt acccgagcgg cggagcatac gccctctccg
     3961 gaaaattcgc cggtgacgtg attagccagc cgacatggaa agggcctgcc gcgaaataca
     4021 gtgtggatag ccggctgacg acggccgcgc tcgccggcgg gtatcgtgtg tgccatacct
     4081 gtctcggcac cagagatcag gagagcacac ttggcgccgt tgatctgtcg gtgctgatcg
     4141 cccagagcgt tggaggcatc ttccattcga tggaagcgta cgannnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnngaaactc agcatgggga tgccggcgag gtggagcgca
     4321 cggtggaaac agtatgccgc gagttcgaag cggcgaaacc atacctcgtg gagcaatgga
     4381 ggtggcgcga tgagtgacct gtggagagag acgatggtcg ccggttttcg tgatacggtg
     4441 atgcaaatcg tgctcgtgtt gccgcggctc ctcgccttgc taacgtttct cagtctgggt
     4501 ctgttggctg cctggtcggt gaaagtgctc acactccggg ttctccggac cttcgggttc
     4561 gattcgtttt gcgaacgatt gggcattgtt cagccgctct cccaggcagg agttcgccga
     4621 ccggtatccc tggtcgtcag cggcgtgctg ttctggatcg tctttttgtt atttgccttc
     4681 atgggcgtcg atgccatgaa ccttccggcc acggcgaatt tgatcagcca aatcgtcgcg
     4741 ttcttaccga atgtcctggc cgcatccctt gtgctcctcg tgggagtact gtgcgccaat
     4801 ttctttgccg aggccgcatt gatcgccgcc gtgaacgccc aggtggagga agcgcgcgtc
     4861 gttgcgggac tggtgcggtg ggggctgctg ctattcacct gcgcgatggt gctcacccag
     4921 ttaggcattg cgaaggaaat tgtcatcgcc gcgttctcca tcaccttcgg cggtgtcgtg
     4981 ctcgccctgg ccctggccgt gggcttgggc gcgcggcatc tcatgcggga gattcttgaa
     5041 cgccgcttca gtcaacacga acacaaggcc gaccagttat cacatttatg aaaaaagcgc
     5101 ggcctacaga tgctcggcga aacgtgccgt ccaagccggc gagccggaag aagcaagcct
     5161 cgtcccctgt cttgggcgag ggccggttgc gcgtggccat tgaacatgtc gagccacaag
     5221 ctgacgacgg gcgctatccg atcaaacgag tggtcggaga gacgatcatc gtcgaagcag
     5281 acctcttcgc ggacggacac gacgtgatcc aaggcattct gcgttcccgt catgaacgtc
     5341 aggaggaatg gctaactacg ccgttgagcc ccctggtcaa tgaccgctgg cgtgcctcgt
     5401 tcgatgtcac ggaactgggc caataccgct acggcatcat gggatgggtg gatcccttcg
     5461 cgacatggca gcgtgacatg cgtaaacggc tgcgggctcg gcaggatgtc acggtggaga
     5521 tcttgatcgg gaagcacttg atcgaacagg cgaccgctcc cgcgcctgac gtatcccgac
     5581 gacgcctgaa ggacatccag gcaagggtgt ctcgttctga tctcgcttcg caggctctgc
     5641 tggacctcct gctcgaagag gacacggcag agatcatggc cgactgtatt gatcacgccc
     5701 tgatgagtac atatgaccgc gagctcatcg tcgtggtgga tcggcccaaa gcgcgattca
     5761 gcgcctggta cgaaatgttt ccccgttcga cgacagacga agaacagccc cacggcacat
     5821 tcaaggattg cgagtcacgg ctcccctaca tcgcggagat gggatttgac gtgctctatc
     5881 tgccgcccat ccatcctatc ggcgaaaccc accgcaaagg acgaaataac aatccgcgag
     5941 gtgactcgtc ttcggtaggc agtccctggg cgatcggatc tcgcgatggc gggcacaccg
     6001 caattcattc ggaactagga agcctgggcg atttcgagca actcgtggct gccgcccaga
     6061 cgaacggcat cgagattgcc ctcgacctcg cctttcaatg ctcacccgac catccttacg
     6121 tcaaagagca tccggaatgg ttcaaaacac gccctgacgg caccattcaa ttcgcggaaa
     6181 atccgcccaa acagtaccag gatatctttc cattacattt cgagacagac gcgtacccag
     6241 acctttggaa agagctacgc gaggtggtcc agtactggat cgaccacggc atccggatct
     6301 ttcgcgtgga caatccccat accaaaccct ttctgttctg gaaatggctc atcagcgacg
     6361 tgaaagcctc ctatccggaa acgatcttcc tggcagaggc gtttaccagg ccgaaagtca
     6421 tgtatcactt agcaaagata ggttttacgc agtcgtacac ctactttgcg tggcgcaaca
     6481 gcaagcagga gctgaccgac tatttcaccg agctgacgac ctcctcagta cgggaatatt
     6541 ttcgccccaa cctctggacg aatacgccag acatcctcac cgagcattta cagcacggcg
     6601 ggcgtccggt attcatggcc cggctggcgt tggcggcgac cttgggatcg agttatggca
     6661 tttatggccc ggccttcgag ctctatgaaa accgtccgca gaaaccaggc agtgaggaat
     6721 acctggattc tgaaaaatat gaagtgcgga gatgggatat caatcgaccg gatagcttga
     6781 aagaattcat cggcttgatc aaccgtatcc gccgcgagaa tcctgcgctg catcgtgacg
     6841 agagcctgca gtttcatccc atcgacaacg agtatcttct ggcctacagc aaacaatcgg
     6901 ccgacggctc gaatgtcatt ctcattgtgg tgaacctcag cccgcaccat gttcattcgg
     6961 gctggctgga attgaatctg gcggctctcg ggatcgaggc aaaccaaccg tttcaagtcc
     7021 atgatctcct gacgcaggcc tattacgtgt ggcagggacc tcgaaattat gtccagctcg
     7081 acccacattc agtgccggca caaattttta cagtgcgccg caatcttcgg cgcgaagagg
     7141 attttgatta ttacctctaa ccgttgcgca ggcataccaa tctcatgatc accgtgcggg
     7201 acaattggaa agtcgccttt gaaggctcgt cacgggcgga aatcgagaag ctgctgccgg
     7261 acttccttac ggcctcgcga tggtttggcg ggaaagcgaa aacgattcgc tcggccaagc
     7321 ttgcagacgt tctacagcag gaacatgggg acggcactat ggtcctggca ttgatcgacg
     7381 tgtcctacct tgaaggagga gcggacaggt acacactgcc gatgacggcc gcttttgatg
     7441 cggaggcaga tcgcgtcaaa catgactatc cagaggcgat catcggggca ctgactgtcc
     7501 tccagaacgg aacggcactc gctggaattc tgtacgatgc gttatggcat ccggactgcg
     7561 cgtactcgct gctggacagc atgggccgac gcgggcaatt ccggagatca atcggcacgt
     7621 tggtcggatc ggctaccgaa ttgtttgatg aagcgtccca ctgcgcgcat gccgcctcca
     7681 gttccgttct gaaaggcgag cagagtaata cgtcggtgaa atttggcgac tgtgccatca
     7741 tgaagctgta ccgacgaatc gagcccggca tgaatccaga attggaagtc ggccgtgccc
     7801 ttacggcacg acactttaca cactccccca gcctcatcgg tgcactggaa tacgtacaag
     7861 aggctggcga gccgatgacg ctcgctctgg cacaaacctt tgtcgccaat cgagggaatg
     7921 cctgggatta caccctggaa caactgtccc gctacataaa tggcatcctc gcgctcgatc
     7981 acaggaagga agaggctggg cagccggcgc atgaccgatc gaatcaggaa cccacgacca
     8041 gtgatcttct tttgggctac cgtgattcgg cacacctatt gggccaacgt acgggcgaac
     8101 tccatatcgc actcgcacaa ccgagtgatg aggccgcgtt tacgccggag ccctgtgcca
     8161 cgtcatatgt acagtcgcgc gttcacgcca tgcaacagtc tgccactcag gccttggcat
     8221 tactgcgacg ccgacttcct tcactctctg tgacggacca ggaacaggct caaaaggtcc
     8281 tggaacagga gtctccgctt ctcagccggt tagcaacact ggcgcggcaa cccttgagcg
     8341 cgttgcgcat tcggtgccat ggcgactatc acctgggaca ggtcctctac acaggcgaag
     8401 attttgtcat catcgatttt gagggagaac cggcaaagcc tttggctgag cgccgggcga
     8461 aatgcctccc catggtggat ctcgccggaa tgattcgctc ctttcactac gccgcccatg
     8521 tggcattgcg cacaggagaa gcacgccgcc ctcacctacc ggcgtcgctc gacttggttt
     8581 cgtggatgga gcactggtat cgaacggtgc gcacggcgtt tctgagagga tatcgaacta
     8641 ccgcagggca ggcgccgttt tcccctcaat caccggacga gttcaacctc ttattggatg
     8701 tgcagatgct ggataaagcc atgtatgaac tgacgtatga gctgaataat agaccggatt
     8761 gggtgggcat ccccctcacc ggcctcctac actcgatgag tgcctctatc gccattgagg
     8821 atgctgacaa cgacgatgtg cgtaacgatg gggccattcc ccgttcaggc ggacaggtga
     8881 gagacgaggt gaaccagtga gtccacagaa ccttgcacag aatccggggc cttcggcaac
     8941 caccagactg actgatgatg atgtctatct cttcaatgag gggacacaat ttcaactgta
     9001 cgacaaactg ggcgcgcacc cgatggtttc tcacggagag gcaggcactt actttgcggt
     9061 gtgggccccg aatgcagaac aggtgtcggt catcggctca ttcaacgact ggaatcgcga
     9121 cgcccacatc ctccatccac gaggagactc aggcctgtgg gaagggttca tcaccgagtg
     9181 cgccaaggga gcgttataca agtaccatgt ccactcgcgc catgccgact atcgcgtcga
     9241 taaaagcgac cccctgtcct tctttaatga aatcccgccg aaatcggcgt cgattgtctg
     9301 ggacctcgat tacacatgga cagacgacga ctggctgcgc acacgcgggc gcgccaatgc
     9361 attgaacgcg ccgatggcaa tttacgaaat gcatgtcgga tcatggaaac gcatcgccgc
     9421 cgaacagaat cggtcgctca gttatcgcga actggcagtc ccgctggccg agtatctgca
     9481 gaagaccgga ttcacgcatg tggaatttat gccgctgacg gatcatccct ttttcggttc
     9541 gtggggctat caaacaaccg gctatttcgc accgaccggg aattacggca caccgcagga
     9601 cctgatgtat ctcatcgata cgttgcacga tcacgggatc ggggttctgc tggattgggt
     9661 cccgtcccat ttcccaactg atgaacaagg actggggttt ttcgacggca cgcatctgta
     9721 tgaacatgcc gacccgcggc aaggatttca gctggattgg aacacgttta tctttaattt
     9781 cggccgcaat gaagtccgca gctttctcat tagcagcgca ctcttctggc tggacaagta
     9841 tcacttcgac ggcctgcgat tggacgcggt ggcctccatg ctgtacctag actactcgcg
     9901 gaaagaaggc gaatggattc ctaatcgata tgggggtcgc gaaaatctcg aggccatcgc
     9961 tttcctcaag cggctgaacg aagaagtgta ccggcacttt cccgacgtcc aaaccattgc
    10021 ggaagaatcc accgcttggc ccctggtctc tcgccccaca tacctcggtg gcctgggctt
    10081 cggattgaaa tgggatatgg gctggatgca cgacaccctg acctatatgc aaaaggaccc
    10141 gatcgatcgc aaatatcatc atcacaacct gacctttcgg atgctctatg ccttccacga
    10201 gaacttcgtg ctaccgctct cgcatgacga agtggtgtat gggaaaggct ctttactggg
    10261 gaaaatgccg ggagacgaat ggcagaaatt ttccaacctt cgtctgctct tcggacatat
    10321 gtactcccaa gccgcgaaga aactcgtgtt catgggagga gaattcggcc aaccggctga
    10381 atggtcccat gatggccagt tggaatggag tgcgctggag ttaccgctcc atcaaggggt
    10441 gcaccggtgg gtgacggatc tcaatcacac gtatcgccgg gaatcgtcac tccatgaagg
    10501 cgatgtcgat ccctccggca tcgaatggat tgactgccgg gacgcggaat cgagcgtcat
    10561 cagtctgatc agaaaaggcc gggccactga cgagatcatt ctcgtcgtgt gcaattttac
    10621 ccctgtgccg cgtcccaatt atcgcctcgg cgcgccacgc ggtggatact ggaaagaact
    10681 cttgaacagc gatgccgcga tctacggcgg ccatggctgg ggaaatgccg gcgggataga
    10741 agcgacacct gtacccctgc atggacgaac gcattcgatc acctgtacct tgccagcgct
    10801 gtctgtcctc ttccttaaac attcttcatc accgctgtga tctcgtcgaa acggtgcacc
    10861 gcggctcctc caactgattg ctgcaattcc ctcgcggtgg aaccgttgca tttcaccgcc
    10921 caccgttgcc aatcacgatc cgcatacccc tcgatccgct cctgacgact gctccctcag
    10981 gaaatcaaaa ggttatagcc gcaatccatg gcgttctccg aggcatgggt gttgctgatg
    11041 taggggcagc aaccatcgat gaccatttgc ccaagcatgc acatcactcc ggcctccagg
    11101 ccgggctacc tgaggaggat cacatgaaga acaccatcgc actgtattcg acagcgttcc
    11161 tgatcggagc ctggcccctg ctggacctgt cccaggcgca ggcattttcg aataaccctg
    11221 aaccggtcgt atggaacagt aatcaaaaat ccgccgcctg ggctgaagag ttgctcggtc
    11281 aagtggtgac ctatcaaacc atggcggaaa aaaatctgat tccaggaagt tttcaaccct
    11341 atgtggaaca gacgagcaag atacgagagt tgcaccggtc cggcaatcga cgtgcgacgt
    11401 atgacggcgt caacgccttg atggtcatgc ttgaggcacg cgtgggcggc attgacgagc
    11461 atagcgccga ggccttgtgg gatttttgct atcgggtgac acccgatgaa ttccacgcgc
    11521 gggatcggca cattcgggcg aaaggcgcgg acactgtgaa agagcacgag gaattcatgc
    11581 gcaacatgga agagcgggcg ggaatgagct tctagtcgtc gttcgtttcg tcgaacagtg
    11641 aatctgccgg gggtgcgcta ccaagtggcc cccggcctat tcgccccgac acttggggaa
    11701 atggaggatt ctatgttcca tgactgtcga gacgccgcat cttcgccatg tggaaattcc
    11761 tgttgttgtg gtttgccttc acacttccat cgggcgcgat ccttgccccg acttccctca
    11821 tggccgaagc ggggcatgtc ggctatgggc cgacctcacc ggcgcttttc tcgtcggctg
    11881 aaagtctggt gatcgacact gaccccgtcg cactactgcc tccccctgct gcctttgaca
    11941 tccggaattt ctggatgcgc gccgacctgc gggtcagacc ggaatggcgt agcggggtct
    12001 gttttggagg aggacctcct atgaatggcg cctgcaacag cctcaacctc tccggatccg
    12061 gcaccagcgc ccacgatggc tccaaggcca gcgacttctt cattcagcaa tgggcgcggc
    12121 tcggcctcgg gtatgatcct tcgcccactc tcaattttta cgttgaactt caggattcgg
    12181 cgacatgggg aggcagcggc aacccgacgg atggaggcca gagtgcagac gccgccaacc
    12241 atcaatgcgg aactcagcga ccgggacaat gccggctcgg gatccgagcg ggctatgcgc
    12301 tcatccggaa tctcgccggt gtcgaaggcc tcagtgcgaa gttcggccgc caatacgtcg
    12361 tgttcggcaa tcaacgtttg ttcggacact tcgattgggc gaataccggc tattctcacg
    12421 atggcgtcat gttgagttac gctcgcaccg attttgatct gaaactcggc tggtttcgtc
    12481 attcggagac tgacctgggc caagggcacc ctggcggaag tctgaccccg aatcttttaa
    12541 cgtgcaacca ggacgtcaca tcccagtccg tctgcaatcc ggccgtggcg cagcatgcgg
    12601 ccgacgcggg aagcgatgtc gacatggtag tgttttacaa tcaaatccga ggcgtgccgc
    12661 gaatgattgt cgagccgtat tacgtcctct actccaaccg gcttccagaa cgagccaatc
    12721 ctgggcagta ccttcccaag tcggcctctc agctcagaca tatggtcggg cttcgtctcg
    12781 aactacgcga tggcaattgg gatctggtgc atgaaacagc gtaccaattc gggcggaccg
    12841 cggacgggtt tggcttcgac aaccagcgca acctgagcat taatgcctgg gcctctggca
    12901 cctggcttgg ccacacgtgg tatgcccatc cttggaagcc acgagtcgcg atcgggtttg
    12961 actatgcgtc gggagacgga gacagcaatt gcgtcacacc cggcggaact ctcgcgcgca
    13021 gctgcggtgg caatgccaac acgttcgaaa atttctttcc cacaaacttc ctgcacgtcg
    13081 gctacatgct gaacggggcc tggaggaaca cggttcagcc gcaggtgaac ctgcaagcca
    13141 ggccgaccac tcgagatcac gtcgaagtct gggccatgcg caagtacttg gcaagcgctc
    13201 gcgacaattg gtaccgtgga tcacaagggc cactcatttt ttcacgatcc gacaaccaga
    13261 cgacccacgt gggcgacgaa ctcgatgtcg catggagtca catgtttgcg gacggtaccg
    13321 tgtcagtggc cgtcatctac ggccattttt tctctgggcc ctatatccgc caccagctcg
    13381 gcacctcaat ggatcaggac tggggcatcg tacaactctg gaccaatttt taattcgtca
    13441 tgcaacgcgc aaccctgaat tttgaaacac ttctcgaagt caccaatgca ctgaactcgc
    13501 agcgagacat cgagagcctc tggcgagtca tcgccgacca aatccagaaa gtgattccat
    13561 gggatcgtgc ggggattacc ttgtacgaat ccaatactga cgccttccgg ttttacgccg
    13621 tcatcaccaa catggccagt ccggccctgg cgcatgatag cgtcattccc cgtgaaggca
    13681 gcgcggtggg atgggtgtat gaccaccggc gcctgcacat tcgtggcaac cttcagaagg
    13741 aacaagtgtt tctggaagac cgtttttatg cccaagaagg attgggccgc atgattaacc
    13801 tgccgctgct ggtgcgtgag cattgtctcg ggacactcaa catcggcagc gtccatggtg
    13861 gcgagcccga cccggacgac tgcaagtttt tgcagcaagt cgccacccag atcgcctacg
    13921 ccatcgatca cgtcctggca taccagcaga tcaaacaatt gagcgagcgg ttgcgccgcg
    13981 agaacgagta tctcgccgaa gaggtgaagg ccagccgtaa tcagcggttg ctcgttggag
    14041 catcttcatc attcagcaag gtggtcgagc ttgtgaaggc ggtcgcgccg acagacacaa
    14101 ccgtgctcct gctcggcgaa accggaacgg ggaaagaagt gctggcccag gcccttcatg
    14161 acttgagcgc gcggagccaa aagccattta ttcgcgtcaa ttgcgcagcg ctcccttccg
    14221 gactgatcga aagtgaatta ttcggtcatg aacgcggtgc ctttaccggc gcacaactcc
    14281 gacgagcagg acgtttcgaa ctggctcaca ccggaacatt gtttttggat gagatcggtg
    14341 aaatgccgct ggaaacccag gccaaactat tgcgggtgct ccaggacggc atggtcgatc
    14401 gaatcggcgg cacgcagccg gtttccgtga atgttcgact catcgccgcc accaatgccg
    14461 acttgtccgc cgcgattcaa cagggaactt tccgagccga cttgtactat cggctccaca
    14521 tctttcccat cgcagtccct cccctccggg aacgccgcga ggatattcag ctccttgccc
    14581 aacattttct cacgcaaatc ggcgcgaagc acaagcggcc gcatctgacc tttgacccac
    14641 agtccatggc acgcctcctc tcttacaact ggcctggcaa tgtgcgcgag ctgcaaaatg
    14701 tgattgaacg cgcggtgatc ctttccagtt catcccaagt caccgtggac gaaatgttgc
    14761 ttccgatcgt ccgggcagga cgggccgtct tgacgcatca gcctgagagc cttgaagatc
    14821 tcgaacggca tcacatcatc atgatactgg aacggaccaa atggcgaatc tatggcgacc
    14881 aaggggcagc caatctgtta ggactcaatc cagaaaccct gcgcagccgc ttacgaaaac
    14941 ttggcattcg ccggcccgtc tcacgatcat cggaaaccag ccccatggcc taactccctg
    15001 acatttcaag catcactctc gtgctccttc gtaagcagca taagatcgat ggttgaacgg
    15061 gaggagctat ccgcctcaat cgccgggtat tgttgaccgc ggacggctca tttgaccaag
    15121 gtgtcgtaag catgataagt tgctaaaccg tacgagtaac gagcgagatc ccacgcttcg
    15181 cgtttccgtc attgccgcgc gatcgcatcc ccaagttacg ctcctcctac ggcccatttc
    15241 ttacattctg acaggaaatg ggcaaagcat ggggcgagag tattcctccc ccactgcacc
    15301 cagtttgatg gcctggacat cgccatcctg ttgttcgccg aaaggagtgt acgatgaaga
    15361 taacgtccgt ctggcgtggc aacaaaccca accataagaa gaagcgaacc gttcccaagt
    15421 tgagaatccg gtggacggaa ctcggcctgg ttgatccctc cgtggaacca catgagacgc
    15481 cgatggatca gatccgccgt gaagtgacgg cactggccag ttccggattt ggtgcagaga
    15541 cccgcgcccg gtcagaacaa acccagacac gtccggcgcg cactctggca cggaccagta
    15601 caacccgaac cgcccgtcgg ccagctggat cataacgagg caagcctatt tgctccccga
    15661 tcggggaagg tgaatagaag gagggaacca tcgcacgagc aggcaaaacg accatcgcga
    15721 aacgcgatcg agaaaaagcc aggcaggtga agcagcggga gaaagaagct cgtcgggtgc
    15781 aacggaaggc cgagaagccg gaacggcctg cgacgtcggg aggagaagat cccgatctgg
    15841 aagggttacg atgggggccc caggaacccc tgtactagca gaggttcctg ggaggccgcg
    15901 aaggggcatc gtcgttaaga tgtcttgacg ataaagcggt cttccccaaa gatcccgcgc
    15961 aactgcttac tgaccagtga gcgatactcg cccttctctt tcgccattcg gaccattcct
    16021 tcggcatccg tcagaatcag aagatcttct tccgcaatca gcactcgatc tcccttgccg
    16081 acgttaatcc ggtactgtgt ccgtccatcg ggattccggc tcggaccggt gacaatttcc
    16141 gtcaatccgt cgatatgtcc cttttgcccg tccaaacgat gctgcaccat cgtcccatcc
    16201 ggaattctga cccactgggt cgcagctcgc ggtgcatctt ggttctgatc actcaccttt
    16261 cttcctccct tgcaacgtag actcgtccaa attaagagac cggcccaagg tcgcttccgt
    16321 ttttgattcc cccattggcc tctcgagatc cgccatcagt tgtctgacgc catccctggg
    16381 cagacccgcc acggcgtcgg cgccgctccg gttcattgcg tggcgcgtga ccatccggtc
    16441 gtgacgaagg cggcagtgaa gccgggcttc cctcggcgcg aggcaccgct tggcccagca
    16501 atcgctcgat ggctcgcagt tgttcctggt cttccgccgt cacaaacgtc gtcgcacggc
    16561 cggtggtcct catccgcgcc gtacgcccga cacgatgcac ataatcttcc gggcaattag
    16621 gcacgtcgta attgatgacg tgcgcaatat ttgccacatc aatgcctcgg gcggcaatat
    16681 ccgtcgcgac taatactcga tacgtccctc gccggaatcc ttccagggcg gctcgccgtt
    16741 gcggcagcgt acgccctccg tggagcaccg ccacccggtg accggccgta tccaacagat
    16801 tgccgagtcg gtcggcccga tgcttcgttc tggcgaatac cagcacggta tcggattcgg
    16861 actgcaggag ggacatcagg agcgcatttt tccgatcatg cgttgtgtga tgcacagcct
    16921 gggcgacgcc ctcggccgtc gttgctgact tcgccaccat gacgcgcacc ggatccttta
    16981 cgctggcctg cgccagcctg gccagatcca taggcatcgt cgcagaaaac agcaatgtct
    17041 gccgctcttc cggcatggca tccagaatct gattgatctg ctgggcaaat cccatatcca
    17101 gcatccgatc ggcctcgtcg agcacaagaa tgttcatcgc caataagctg atcgttccat
    17161 tccacatatg atcgagcaat cgccccggag tagccacgat aatatccgga cgttgccgca
    17221 accctctcac ctgagcctgc atgtccgctc cgccaaccac cgtggtggca aacaactgca
    17281 ggtcgcggcc caatgtatcg atcgtcgctt gaatctgtat cgccagctca cgcgtcggag
    17341 ccaaaatgag cgcgcgtggc tgtcccttcg gtgtgccggt caaccgctcc agcatcggaa
    17401 taacaaatgc cgcggttttt cccgttcctg tctgtgcgca gcccagcacg tcacgccctg
    17461 acaaggcatg cggtatggcc tgagcctgaa tggccgtcgg ttcagaaaaa ccagctttcg
    17521 tgagatttcg caacagggtg ggagacagac caagggagtc aaaactcgta aacgcagtcg
    17581 tctgcacgca gctatccttt cgttgtggat cgcattaaga cgggatcaga gaaccgaggg
    17641 gagggtgaat ccggagggag tcagctccaa actggacctg gtcgcgatgc gatcgcgcgg
    17701 tccacgttga ggaggtacag cattggtgga ccgatacaat gctgcagtag aacacacttt
    17761 accgcacggt gccccaattg gtcaacagga gaggctgttt caggcggcca agacgcttgc
    17821 cctggcttgc tgtggtagca gaatgcttta tactagtatc acgaatagga gggcacgtaa
    17881 tgcggatgat cctggctgca ctgtgtttgt gcgtgagcac ggcgctgtgt accgtatcat
    17941 cctatgcggc cgagaactcc ggcggcggaa agaacggatc taatcaaggg atgaccctcg
    18001 atgatatcgg gcgcggactg aaaagtgcag ccaaaaatat cgaggaagaa attccgaagg
    18061 tcggccctgc gatcagtgac accttcaaaa aggtaaggga cgggaggaaa gaagattcct
    18121 cagccaaagc atcccctcag cactcgtcca aagacaagaa atagcgctcc ctccaaaatg
    18181 agacccattc atcctgtcgc atgcttcttg gttgtgctgc acaccaccgc cggctgcctc
    18241 gctactcaat caccggtctg tcttcccggc gagcgtcacg ccataatgga gtcaatctac
    18301 ttcgggacag cgacacctcg cggagtggtc agccctgatg agtgggcggc cttcgtcaat
    18361 gacacggtca caccggcatt tccagaaggc cttacctcat ggcaggcttc gggtcagtgg
    18421 cgaaagcaga atgcctccat cgagcacaag gattcgcaca ttctacaact aattcatgag
    18481 gggaatgcgg tgaaaaacac cgccatccaa cgcctcataa gcatttacaa gcagcgattc
    18541 gagcaggagg cggtgatgag agttcgttcg ggcgcctgcc tttcgttccc gcaatagctc
    18601 agacgcgccg cgtgactagc cgattatctg tcctttcctt ttttgatctt tcccacgtca
    18661 tttaaaattt gttgcagcaa tttactactg tttaagcctc cggattgagc cggccccgtt
    18721 cctccaacac cggcggcttg aagcgcgccc cctgtcggcg gagagatgat tgtcgaccca
    18781 ctggttgatg ctccaagcgc agaagcttga cctgatgttg ggctggcaga aatgagcggc
    18841 agtccgatac tagtggcgag gatcgttcct ttcgtagcag aaccgtaagg ctccctttcc
    18901 gctccatccg atgaaggcct ctggaggttc ttctcaaatt cgagggcatg cccgacttgt
    18961 gttccgacga tctgcctggt gacagcatca ggaag
//
LOCUS       DAOQEW010000061        18937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_744_length_18937_cov_8.526798, whole genome shotgun sequence.
ACCESSION   DAOQEW010000061 DAOQEW010000000
VERSION     DAOQEW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18937
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_18937_cov_8.526798"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLS35_08565"
     CDS             <1..347
                     /locus_tag="PLS35_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18753.1"
                     /translation="VAPIVTDAYSGRWQVRARAAMKTPPGPWSSPVPFQLTPGAPNKS
                     LTPALQQTPLSGSSVMQAPPATAGGAASSTRPPTTSSQGGASSLFVRPRGVEGTSKKE
                     GNAPAPQSQREK"
     gene            407..1390
                     /locus_tag="PLS35_08570"
     CDS             407..1390
                     /locus_tag="PLS35_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18754.1"
                     /translation="MRPHWRLGIWLITAPLTVAGVGVLHAEQPAIQCGPPRIEASFTA
                     ALSSYHVTTSCSAVPAGFVNWDSRGSYDSHTGLAREEVHVYLPQGFSATATTTVTCPS
                     DPWLGPAVGAGKVACANGSFNTSGQAQDARARFWLDWLRDGFSGSALPNSTGFSYNRP
                     PLIAQRDAALKTETDAAAALQKHNQQILGAARQGPAVVAPVIVLPAANASFLENTTIP
                     IKLLPPAGMQVTSFMVRLERRDSQGNWAVFNNLPVNLAEATSPTGYTGWGAPGTGRGP
                     AMIAGRGSYRVSAQVLSPKPTRWSVPVEFTVTALNKAIQPGPKAFSPGVVR"
     gene            1450..1908
                     /locus_tag="PLS35_08575"
     CDS             1450..1908
                     /locus_tag="PLS35_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18755.1"
                     /translation="MRRSRPLLLLYALLCLGSALISTPSLAGPPDPCSLLTPAEVEQV
                     VGKLNGAPTSETLGHGVRCGYNFADEKNEFEIWTSEASWSKALRKDAKQPVMVSGLGD
                     EAFMDRGKVDPEAVDLYIRKGATLVLLMTRNGPRVEEQLKTLGRKAIGRL"
     gene            1952..2419
                     /locus_tag="PLS35_08580"
     CDS             1952..2419
                     /locus_tag="PLS35_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18756.1"
                     /translation="MTRISRRVLFIYLSLAVLVSATPTSLSAAPPNVDPCTLLTIAEV
                     EQTVGALKGKPTGDKEGDAAWCNYEFANGKDALEVWVFPSGAIGRSRAISKNSVTVKG
                     LGDEAFMDRGMHGLNYVNLYVKKGSTTINLSIQETTGDEEKVKALAQKALRRF"
     gene            2735..3490
                     /locus_tag="PLS35_08585"
     CDS             2735..3490
                     /locus_tag="PLS35_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNI18757.1"
                     /translation="MNRCHSLIATTALILLFTHATLAGSATSSELDPAARQSMEQVIE
                     EYIRAHPEVIEQSLQALEAKREAEEKAKQKVALAAHQAELLNDPTSPVSGNLSGDITL
                     VEFFDYRCGYCKRAAGAVTQLQQENPRVRVIYKDFPILGESSELAAKAALASKAQGKH
                     QVFHEAMLAFKGDITKESLLSLAGEVGLDTTKLEADMRNPEWQKAIDTNRALARTLGI
                     SGTPGFIVGTELVPGALDLNGLKELIARANHKP"
     gene            3677..4843
                     /locus_tag="PLS35_08590"
     CDS             3677..4843
                     /locus_tag="PLS35_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18758.1"
                     /translation="MNRYLAIVVTGWLLAGWAGCAADSPSSNSNATTIAHTEEATGQP
                     QADEVQERGVTPKPLRPGLTSPQVFTPIPSAPNTASNPPAFGLLPGDFAIRSYLKNTP
                     LTARDGGHHSIDAVITLDSVIGPNQRFRLATVQPDYTTIQTNGGYFVSAVGGLGGLPN
                     TTQGVQTELQTPQRDIALFRIDGPSPAGTFTIKTYDGHFLTALGGGGKSTDAFHTDAV
                     QANSWEYFYFLKCGDVGSGYQYAIRPTGTGNIPGKGDFVSYLTALNGGGMASQAVTAF
                     SGLQVGSRFRLLRQSDGSYALQTSNGVNYLTAVGGGGIASGDNLHTDARQIQAWEKFK
                     IVEQPNCSYTIQTVSGFYLAVGPGSSSISTRISDPNAAPSIGYNAKFELVMVGL"
     gene            6128..6562
                     /locus_tag="PLS35_08595"
     CDS             6128..6562
                     /locus_tag="PLS35_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18759.1"
                     /translation="MHRLCSHPSLMAGLVCGVIVAAAPALAVPIKNPDNCKGPVTIGV
                     ATCPDKPGHWVECIPSGDYMCCVQNAQGGKDCEQIEALKGPRGGMTRVPGGMLQNAPV
                     VSPAPINPRVPRTGMNAPIMRRGIEDKPSGDGRPGTDDNENK"
     gene            6618..7079
                     /locus_tag="PLS35_08600"
     CDS             6618..7079
                     /locus_tag="PLS35_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18760.1"
                     /translation="MTKPYAITTVSLMILTGLLALPAASDAKPRKPLVNGCTVDQIQS
                     NFGNSCVDQMQQDIMGNKPYTHALLCSGGQMLCCTYDNTTNQIQTCRKPAGSAFMPGV
                     QGSVGVLGSAGIQRRGVDANTAAEAEEAPAPEWMTPERMQQLQQDFKTNGQ"
     gene            7166..8233
                     /locus_tag="PLS35_08605"
     CDS             7166..8233
                     /locus_tag="PLS35_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18761.1"
                     /translation="MTIVRYPCTACLVFVMAWGLSMACQAQDNQDSLPSSAPAQEARP
                     NSTEGTTPDEGIKIQEPDLITDDHHPVTGEIQERGIVDKFQATPGKTFMQAQYQAPTA
                     NLTLVANALRLAYKSVSTLVTIAPNLPVTQPVEISIGYYSAAGNDRITQSYVRASGNR
                     FLYNDKEGDGKPRTMTISISLREPKAGGGFETFAVNWQAPLDPLYDVSIGPFAFDLIS
                     KCDAVGKSEILFTWYAPDQQYHKFRFSTRAGSRTTISPFAWSRQEVSWSHKVSRPTET
                     FYDEDNAATEMLWNCLPAGCGFTVAGGPSAQHLLTGASAMVKGNLKAMNDNCQAYFEY
                     PMTKTLRWYPYLDSPTVRDHR"
     gene            8313..9545
                     /locus_tag="PLS35_08610"
     CDS             8313..9545
                     /locus_tag="PLS35_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18762.1"
                     /translation="MRHTAVVTFVGTAALCLGLAGCAGTDHSKDQAARDVPAREVPVI
                     PEDAPATQDAIQSAESAEVQERGVSRVLPGGTAGAAVLGTLSTPFTSAYPGEFAFRTQ
                     KGYYLTAVNGGGRTGDPTIITGATSAGAWEKFRIGIATPPSPYDRLLQTATGNYLTAV
                     NGGGMTANVLHTDATQPKEWERFRLVYMGGYPPPYYGIGTSKGNFLTAVGAGGRYQDA
                     IHSDARQVQAWEQFRIVKCGDPGSGYEYGIMAADSELFAASTYQGKTWFGLNGHPSEA
                     EMKLKVIRQGDGTYALQTTSGRYVTALGGGGQVEKYDRKYWTPDCGGLFGGPCVGGAS
                     PIFHIDATQVQAWEKFRLVDQGNCTYTIQTTSGFFAGIFKDGSGVTLLTTRRDGAPTA
                     NEKFQLVVFGLASPPIIQ"
     gene            9631..10086
                     /locus_tag="PLS35_08615"
     CDS             9631..10086
                     /locus_tag="PLS35_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18763.1"
                     /translation="MRSDRVVFIGAISLLTFAVILPAPASARPKGTWQSCNTKDLNTN
                     FGASCNDQMQQDIMGNKPYTHVLFCGGETMLCCTVDNNTNQVINCRKPAGSRVMPGLQ
                     GNTLGASGMAGVQRRGVESKAQDSDEDAPVPSTLTSDVVKELRPKAAAK"
     gene            complement(10220..>11014)
                     /locus_tag="PLS35_08620"
     CDS             complement(10220..>11014)
                     /locus_tag="PLS35_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18764.1"
                     /translation="MPGPRQQQPVMPGPGLQPMMQPPQVTSPLVKTIPLALLLGGDLS
                     KSFVQLTNLRGQVDSNFTIGRSLAGRFTVPPYVHEASNGSRVFVHANGLSNPPVVKSE
                     GNELHLEFHLPALQFKTYYRDYSPEGDQAVGDTVGENAIIDIFILPTTNQQGFPTYQA
                     VRAGFRGTLKPQDKCTYWFDVIFPINICEVSKKYLDTLSNAIETGVREALSHPQTRTQ
                     FDQIVWQSLRSDLLIQAGINPMSPAQIRVIEASFHGTDYVVKYLPR"
     assembly_gap    11015..11114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11509..13110)
                     /locus_tag="PLS35_08625"
     CDS             complement(11509..13110)
                     /locus_tag="PLS35_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNI18765.1"
                     /translation="MHTRLQPLRTASGTRTSLLIFLPFLLLASCAAAPFLIPAGIEFA
                     RNLLVTSNKNYGGKYSEDMNRLMVRLSTPYVAMGLPMGTSPAALVPPTMLMQQQQAMM
                     NAQASGQAGMGMGMGYPDPYNPAGAMMGGYGAQGYGGMGGYGGMSGYGGMGGYGTSQY
                     GMSQYGYQGQAGGYYDPNNPYASVPGMNVQAPAYGMPQPYGGQYGAQSYGGQMGGYPP
                     QPGYPAQQGLPPQPGYPSAAPGMAAQAPAPGYPPQGMQGYGQQPMQPGMYQQPMDLYQ
                     QQAMQGYQQQPMQQAAMGGYQQPAVQGYQQPVQGYAQPGMAQPYGQPPNVGVSYPAGV
                     YPRGIPGPAEPVALDVALIRQVNGSNGKQVALMQDGETLKGGQDGDRFKLVVRTNCEC
                     FVYVISIDGSGWAQSVFPVANGPVNNPLKPEVEQAFPDGPYWFTLDQFKGVETFFLVA
                     SPARRTDLEESLGQLAGQQRPAGQVAAQVEEPAVIPNGFGKTQSGQAAQVLDDGQQAM
                     QVTPLSYVAAKVGDDVRVTRWFRHQ"
     gene            13280..13864
                     /locus_tag="PLS35_08630"
     CDS             13280..13864
                     /locus_tag="PLS35_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI18766.1"
                     /translation="MDATHGHNALERTLVARVATGDTQAFEALYRLYDKRVYHYIQTF
                     IRDAALAEEIVGEVMFALWRGACTFTHQSRVSTWIFGIARHKALDAVRRHGRIDHSTA
                     PLDDVPDLPSQQETPIDGIQREQLGIVTRQALSKLSAEHQEILRLVFYEELPYEEIAL
                     LLSIPENTVKKRVFYAKQQLKRHMDRMLSRESVV"
     gene            13861..14538
                     /locus_tag="PLS35_08635"
     CDS             13861..14538
                     /locus_tag="PLS35_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNI18767.1"
                     /translation="MTTPHNLAPPHPTATLLPWYLTGTLKESERQAVDEHLASCADCR
                     AELAHLTHLRTPVKTAFAEETTPELRVQQQVMIRIQAETLPAHSAGPTTSKEGAAESV
                     EQWFRRLFAPRWIPALAAILLIGQLTLLLWTAGEQTALPPSHIASRGIQPVSSKIVLV
                     FQDSIPEARIRATILALKGRLVDGPTVDGRYTIEIPSTETAALDQQLHTLQQQSAVIR
                     RAERLAP"
     gene            14588..16819
                     /locus_tag="PLS35_08640"
     CDS             14588..16819
                     /locus_tag="PLS35_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI18768.1"
                     /translation="MTHRFSTRTILSLAVITLLVVQPVPSFTTEPPSSGTKRALLIGI
                     NRYQVLPTLNGSLNDIDTMRQVLTTRWGFQSQHITMLTDQAATRSGILAALERLVQDT
                     GPSDVVYVHYSGHGSQVEDLNGDETEDHLDETLVPHDGRTPSVPDITDDELEAIVSKL
                     KARSILIVLDSCHSGTATRSLDIRTRSVPQDTRIELYRPSGVHTRQVVPVTAARYVLM
                     TGAAANQEALDGPVGGRYHGFFSYSLSRSLGSAGLSASPREIFFGVEREMKRIQTHFG
                     RISMPEPQLEAPPALMDAPLLPMPSETGNETGTNGTARLPWVEVKAHKGGETLLLNGL
                     QLGAAPGSTWAVYPPNETNFAPGKSLGLLTVTQSRGKDAVTAPHATAFTIPTGSRAIE
                     FLPPASVEGVPVRLLEIPNDRREKIEQVLREQGREVQIVGPGQPAQFLVEVKGNRLQL
                     YSADGLQLVSSFSLDNAQWGAGLARVLSRSANASELLTLDNPSAQIKLDVRIAGAARR
                     QARGIAVVADTQPAQYRIRHEGEPRTPGNSLQLEIQATADSYLTIVDVDSEGHLNLLF
                     PTVHQQPSFYPEGRVRAGDVVTIPDSLQSGNHAGFHWDYAPPAGTDTVRVFASTDLET
                     AQAIRQRVIATTPPGPLATTMVTTRSILSSSMTHLRQDLSRIATRGLSSVYDPTPAAL
                     TNQPSTPPTQTPTAPTAGPSSPQPPVSQNLSPPIAPSTAQTSALTPAADWAATSLTIE
                     VAP"
     gene            complement(16986..17966)
                     /locus_tag="PLS35_08645"
     CDS             complement(16986..17966)
                     /locus_tag="PLS35_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNI18769.1"
                     /translation="MSALLILLLTIAGWSFTAQAWAFGDLGHRTVGAIADAQLTPETR
                     AAVWTLLRSGETLATVATWPDEVKRAKKNLGPLAHDAEAQRFNEDFPDNREWHYVDLP
                     LDTAVYTEGAVGSRLNDIVQTIQRCIRVIESQTVSDHEMTRAQALRWLVHLLGDLHQP
                     LHLGSGYYQFDAQRQAHLITDPHEAAGKDEDRGGTLLLTAAEEQLHAFWDDRLVNEVA
                     KGRSYLELAQDLLAQGHQGAWDGDGSYHHWVDQWAVEGIRLGKSAYERLVFGTAQFDG
                     DHTGLRKITITLPRGYEQDEAKIVEQQLAKAGYRLAAILNALRFPAAASH"
     gene            complement(18236..>18937)
                     /locus_tag="PLS35_08650"
     CDS             complement(18236..>18937)
                     /locus_tag="PLS35_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18770.1"
                     /translation="ATYRDPKTGADISTKNSWIADVIVDRTIQGYETRQNYRDVALFF
                     QDEDNIIGTSFMPYVQNTAGLTAVNYRAEPYKFREASGCSLGKVFQPCSVDKPESIAT
                     PIIEAHAGDPVRIHVFGASNEQNGMFSVEKHEWPIEPFMRGADQISVVEFSASESLDA
                     FIQAAGGSFRQPGDYVWSNQRLPYAQSGQWGLLKVLPTDDQRILPLSQQAPSVKRAGS
                     ESGEAHASKTSHLIQ"
BASE COUNT         4100 a         5762 c         5384 g         3591 t
ORIGIN      
        1 tcgtggctcc gatcgtcaca gatgcctact ccggacgctg gcaagtgcga gccagagcgg
       61 ccatgaaaac tcctcccggc ccctggagca gtcccgtgcc gtttcagctg acgccaggcg
      121 ctccgaacaa atctctcacg cctgcattgc agcagacacc cctatccggt tcatcggtga
      181 tgcaggcgcc gccggcaacc gcgggcggtg ctgcgtcgtc cacccgaccc ccgacgactt
      241 cctcccaggg cggcgcaagc agcctcttcg tccggccacg tggagtcgag ggcacaagca
      301 agaaggaagg caatgcgccc gctcctcagt cccagcggga gaagtagtcg tgaacgccgc
      361 aatccatcgg gcgaatgaac accattcggt catacaggga ggatctatgc gccctcattg
      421 gcgactcgga atatggttga ttacagcgcc cctcacagta gcgggtgtcg gtgtcctgca
      481 tgcggagcag ccggcgatac aatgtggccc tcctcgtatc gaggcaagct tcacggcggc
      541 cctctcaagc tatcatgtca ccacctcgtg tagcgcggtg ccagccggat ttgtgaattg
      601 ggactctcgc ggttcctacg actcacacac gggactggcg agagaggagg tgcatgtcta
      661 cctgcctcaa ggattctccg caacggctac caccaccgtg acctgcccga gcgatccctg
      721 gctggggcct gctgtcgggg ccggaaaagt ggcctgcgcg aatgggtcgt tcaacacgag
      781 cggtcaagca caagatgctc gagcccgctt ctggctggac tggctgaggg acggattctc
      841 cggctccgcc ctgcctaatt ccaccggctt cagttacaac cgaccaccgc tgatcgctca
      901 gcgcgatgcc gcgctcaaaa cagaaacgga cgcggccgcc gcgttgcaaa aacacaatca
      961 acagatcctg ggcgctgccc ggcaagggcc tgcggtcgtc gcaccggtga ttgtcctccc
     1021 cgccgccaac gcgtcatttc tggagaatac gaccattccc attaaactgc ttcctccagc
     1081 agggatgcag gtgacgagct ttatggtgcg gctcgaacgc cgggatagtc agggcaactg
     1141 ggcggtcttc aacaacctac cggtcaacct ggccgaagcg acatcaccaa ccggatatac
     1201 aggatggggt gctcctggaa ctggcagagg tccggcaatg attgccggcc ggggcagtta
     1261 tcgtgtcagc gcacaggtgc tgtctccgaa gcccacgcgc tggtctgttc cggtggaatt
     1321 caccgtgacg gccctgaaca aggccatcca gccaggaccg aaggcgttca gtccgggagt
     1381 cgttcgatga tcgtggaccg actccatttg gcctggacac accacgtttt catcgtgaag
     1441 ggaggcacca tgcggcgctc acgtcctctc ctgttgttgt acgcattact ctgcctcggc
     1501 agcgcgctca tttctacgcc aagcctggcc ggccctccgg atccctgctc gctcctgacg
     1561 ccagcagaag tcgaacaggt ggtcggcaag ctcaacggcg ccccgaccag cgaaacactg
     1621 ggccatgggg tgagatgcgg ctacaacttt gccgacgaga aaaatgagtt cgaaatctgg
     1681 acgtctgaag cctcctggtc gaaggcgttg cgcaaggatg cgaaacagcc ggtcatggtc
     1741 agcggactgg gcgacgaagc cttcatggat cggggcaagg tcgatccgga agccgtggat
     1801 ctgtacatca ggaaaggcgc gaccttggtt ctactgatga cgagaaatgg tcctcgcgtc
     1861 gaagaacaac taaagaccct tggcaggaaa gcgataggcc ggttgtaatt ccccgaattg
     1921 gatttccaag ttgccacgtg aaggagtcgg catgacacga atttctaggc gcgtcttatt
     1981 catatacctg tccttagccg ttcttgtttc ggccacacca acatcactat cggcggcgcc
     2041 tcccaatgtc gatccttgca ccctcctgac gatcgccgaa gtggaacaga ccgtcggtgc
     2101 gttgaagggc aagccaacgg gcgacaaaga gggcgatgcc gcctggtgca actacgagtt
     2161 tgccaatggc aaggatgcat tggaggtgtg ggtgtttcca tccggggcca tcgggcggag
     2221 cagggccatc tctaagaatt ccgtcacggt aaagggtctg ggcgacgagg cattcatgga
     2281 ccgtggtatg cacggactga attacgtgaa tctgtacgtg aagaagggca gcacgaccat
     2341 caatctgtcg attcaggaaa ccacgggcga tgaagaaaag gtcaaggcgt tggctcagaa
     2401 agcgttacgc cggttctgaa ggaaatacct gcattattcc atcacgcagt tgacccagaa
     2461 ctgcggtgct gcgtatgctc cagcttcccg aagtccggtc gcaaaggctt accgggcgtg
     2521 gcttcgcgag gatcagcatg attccaccat ctgccgatta tccagctgca ccgccctctc
     2581 cagccactct tatatctccg ttgaaccttt caccttgcgc gtccgacata cggcgcatga
     2641 acgaaatcac aaggatggat cgccgagtgc tccgaccgtc cgcgctgcgc cacacaacca
     2701 gacaccgcac catgacgaga ctgaaggagg gccgatgaat cgctgccatt ctctcattgc
     2761 caccaccgca ctcatcttgc tgttcactca cgccactctg gccggatccg ccacctcatc
     2821 agagctggat ccggccgcac gccaatccat ggagcaggtg atcgaagaat acattcgcgc
     2881 ccatccggaa gtgatcgaac aatcgctcca ggcattggaa gcgaaacgcg aggcggagga
     2941 gaaggcgaag cagaaggtgg cgctggcagc ccaccaggcc gaattgctca atgatcccac
     3001 gtcacccgtg agcggcaacc tctccggaga catcacgctc gtggagtttt tcgattatcg
     3061 ctgcggctac tgtaaacgcg cggccggcgc cgtgacgcaa ttgcaacagg aaaacccgcg
     3121 tgtgcgggtc atctacaaag actttcccat tctcggcgaa tcgtctgaac tcgcggcgaa
     3181 agccgcgctg gcctcgaagg cgcaaggcaa gcaccaagta ttccacgaag cgatgctggc
     3241 cttcaaaggc gatatcacca aagagtcgct gttgagcctg gccggcgaag tcggcctcga
     3301 caccaccaag ctggaagccg acatgcgcaa tcccgaatgg caaaaggcga tcgacaccaa
     3361 tcgcgccctg gcacgcactc tcggcatcag cggcacgccc ggattcatcg tgggcacgga
     3421 attggtccca ggcgcattgg acttgaacgg gctgaaggaa ctgatcgcac gggcaaacca
     3481 caagccttag ccgtgtcttg aggagtgatc ggggatcaat gttcactggc gtctcgcgcg
     3541 gctcgatacg caccgtgccg gaccggggct cagggcattg ctgtcgatgg atgtggtgag
     3601 aacgacgagc ggagatgatc ccgtcgcggg agtggaagag ttcagagtgc ctcacgaaca
     3661 aaattggagg tgccgcatga accggtatct agccattgtt gtcaccggct ggttgctcgc
     3721 gggatgggcc ggttgtgctg cggattcgcc gtcatcgaat tccaacgcta ccactatcgc
     3781 ccacacggag gaagccacag gtcaaccaca agccgacgag gtgcaagagc ggggcgtcac
     3841 cccgaaaccc ctccgacccg gcctcacctc cccgcaagtg ttcaccccga ttccgtcagc
     3901 accgaacacg gcatcgaacc cgccggcgtt cggcctgtta ccgggcgatt ttgcgatacg
     3961 gtcgtacctg aagaacactc ctctgaccgc ccgagatgga gggcaccaca gcatcgacgc
     4021 cgtcatcaca ctggatagcg tgatcggacc caaccagcga tttcgcctcg cgaccgtcca
     4081 accggattac accaccatcc aaaccaacgg cggatacttc gtgagtgccg tcggtggact
     4141 gggcggcctg cccaatacga cacagggggt acaaaccgaa ctccagactc cgcaacggga
     4201 tatcgcgctg ttcagaatcg acgggcccag ccctgccggg accttcacga tcaagaccta
     4261 cgatgggcat tttttgaccg cactcggtgg aggcggcaaa tcgactgatg cgttccatac
     4321 cgatgcggtt caggccaact cttgggagta tttctatttc ttgaaatgcg gcgatgtggg
     4381 gtccggctac caatatgcca ttcgccccac cggcacagga aatattccgg ggaaaggcga
     4441 ttttgtgagc tacctcaccg cgctcaacgg aggagggatg gccagtcagg ccgtgaccgc
     4501 cttcagcggc ttacaagtcg gatccagatt caggctcctc aggcaaagcg atggatccta
     4561 tgcgctgcaa acctccaacg gagtgaacta tctcaccgca gtaggcggag gcgggatcgc
     4621 cagcggcgac aacctgcata cagatgcacg acagattcag gcctgggaaa agttcaagat
     4681 cgtggagcag ccaaattgct cctacaccat ccagaccgtc agcggttttt atctggctgt
     4741 tggaccgggg tcctcctcga tctcgaccag gatcagcgat ccgaatgccg cgccatcgat
     4801 tggctataac gccaagttcg aattggtcat ggtgggactg taagtacgga cgcacgaagt
     4861 gtcctgacgc agaaacatat caccggatta tttgatcgcg cggagcacca gggctgatga
     4921 ggcctagcat ctgaagtgag catccgtatg gagaacagga ggctggaatg aacactccca
     4981 ctacatgcac cgagagccga ggtacgatac gtgcgcggag tcgcgggcgc tggtttgtcc
     5041 taactggtgc aggcgtatgc acgatactca gtctcttcct gcagtcagat atcagttggg
     5101 ccaaacccaa aaagaacact tactggtcgt gtaaatgcag ctgccgctgg gtagacgaac
     5161 tcgggaaaga gcatttcggg ccatcggggg ccgtgcagtt tactgaatca agcttggaag
     5221 cctgccttgg ccattcctgc acaactacca caccgaccgg cacacaccag ggcacgacca
     5281 gagattgcat gggcaccgag cagcaaagtc agatgcgact ccccccaggt ggggtgcaag
     5341 gacaacttca accagcccaa ccggggctag gaagagtccc tgcccctgtt gtccccggaa
     5401 ccatcatgcg gcgcggagtg gacggcgacc aaccgacttc ctcggaaaaa gaaggaaaat
     5461 aaaggtgagc gagagaatgg aatcatggac atgaaatcga cacggttcgt cacatggggc
     5521 tgggtgatgc tcatcttcgt actcacgacc ggctatcctg tctctgcatc cgctgaacca
     5581 gtggaggcac ccgctgtgaa ccaaccgtcc ggtgaagaga tccaggagcg cggcgtggca
     5641 ccagggctgt tgggggaaat gaaaaaagac cttcaacgaa ggccccttgc ccctggtccg
     5701 gcaggtccgc cgccacaact ctgccatacc gaaacgcaca tgatgaccca gtgcaagtgt
     5761 ttcaaccagg cggactgcca aagcctcact gtgctctttc ccaattcctg cccgtctgga
     5821 agtactcact gcgaattcat ccccatgagc cgcggtccga tgccggccct gccgccgaat
     5881 ctgtgcagtt accaggtccc gtttcctgtg acccgatgcg cctgccatac ccaggcagaa
     5941 tgccaattgt tgtcgccatt ttgtcccgca gcctgtccgg caggaagtca gagctgcgaa
     6001 tgcacgccat ttcaacggcg gtaaatagag tactgacact cggaaccatc gaattgccga
     6061 aagaggaccg acgatcctga tgttatcgct aaacgaattg aggagtcaca gaacgaagga
     6121 ggccgccatg catagactat gttctcatcc aagcctcatg gcaggactgg tgtgtggagt
     6181 cattgtcgcc gccgctcccg cgttggccgt gccgatcaag aatccggaca actgcaaggg
     6241 gcccgtcacg attggagtgg ccacctgccc cgacaaaccc ggccattggg tcgaatgtat
     6301 tcccagcggc gattatatgt gttgcgtaca aaatgcgcag ggcggaaaag actgcgaaca
     6361 gatcgaggcc ctcaaaggcc caagaggcgg gatgacgaga gttcccggcg ggatgctgca
     6421 gaacgcgccc gtcgtatcgc ccgcaccgat caatccacgc gttccgagaa ccggcatgaa
     6481 cgcgcccatc atgagacgag gtatagagga caaaccctcc ggcgacgggc ggccaggcac
     6541 ggacgataat gagaataagt aaaacaacac cgacattcga tcattgacgt gttcagatga
     6601 tcgagaaaga gtacaccatg accaagcctt atgcgatcac aaccgtttcg ctcatgatcc
     6661 tgacggggct cctggcgctc cctgcagcgt cagatgccaa accgcgaaag cccctggtca
     6721 acggctgcac cgtggatcag attcagtcca atttcggcaa ctcctgtgtc gatcagatgc
     6781 agcaagacat catgggaaac aagccctaca cccatgccct tctctgcagc ggcggtcaaa
     6841 tgttgtgctg cacctacgac aataccacca accagatcca aacctgccgg aagccggcag
     6901 gctcagcgtt catgccggga gtccaaggaa gcgtgggcgt gctcggcagt gccggcatcc
     6961 agcggcgtgg cgtcgacgcg aacaccgcag cggaggcaga agaggctccc gctccggaat
     7021 ggatgacgcc agagcgtatg caacagcttc aacaggattt caaaaccaac gggcaatgag
     7081 cagagcttgt tataacgacg ccgcgtagag gttgcatgcg agacagcgtc gcagcctgca
     7141 ggcacaagga caaaggagta gatgcatgac gatcgttcgg tatccctgta ctgcatgctt
     7201 ggttttcgta atggcttggg gcctgtcgat ggcctgtcag gcacaggaca atcaagactc
     7261 cttgccctcg tcggcacccg cacaggaagc gcggccgaac tcaactgagg ggaccactcc
     7321 tgacgagggc atcaagattc aggaaccgga cttgatcacc gacgatcacc atccagtcac
     7381 tggcgagata caagaacggg gcatcgtgga caagttccag gcaacacccg gaaaaacgtt
     7441 tatgcaggcg caataccagg ctccgaccgc caacctcacc ttggtggcca acgccttgcg
     7501 actcgcttac aagagcgtga gcacactggt caccatcgcc cctaacctcc ctgtgacaca
     7561 gccggtagaa atcagcattg gctactattc tgccgccggc aatgaccgca tcacccagtc
     7621 gtacgtgcgg gcctcgggca atcggtttct gtataacgac aaagagggcg acgggaagcc
     7681 gcgcaccatg accatctcga tctccctgcg ggaacccaaa gccggcggag gcttcgaaac
     7741 gtttgccgtg aactggcagg ccccgctcga tccattgtat gacgtcagca tcggaccatt
     7801 tgcgttcgac ttgatcagta aatgcgatgc ggtcggcaaa agcgagatcc tcttcacctg
     7861 gtacgccccc gatcagcaat atcacaagtt tcgattcagc acccgcgccg gatcccgcac
     7921 caccatcagc ccgtttgcct ggagccgtca ggaagtgagc tggtcgcaca aggtttcacg
     7981 tccaaccgaa acgttttacg atgaggacaa tgccgcgacg gaaatgttgt ggaattgtct
     8041 tccggccgga tgtggattta ccgtggcggg cggtccctct gcacaacatc tcctcaccgg
     8101 cgcgtctgcg atggtcaagg gcaatttgaa agcgatgaac gataattgcc aggcctattt
     8161 tgaatatccc atgaccaaga cgctccgatg gtatccctat ctcgatagtc ccacggtgcg
     8221 cgatcatcga taggctgcag gaccgcgaat gcgaagccat accgggaaac tctcacagga
     8281 tgcaccatca ttttcggacc aggagggctg agatgcgaca tacagcagtc gtcacattcg
     8341 tgggcacggc ggccttgtgt ctgggactgg ccggttgtgc ggggacagat catagcaagg
     8401 accaggcggc acgcgatgtg ccagcacgcg aagtacctgt gattcccgaa gatgcgccgg
     8461 ctacgcagga cgccattcag tctgccgaat cggccgaggt ccaggagcgt ggcgtttcac
     8521 gcgtccttcc gggaggcacc gcgggtgcgg cggtattggg cacgctctcg acaccattca
     8581 ccagcgccta tcctggagaa ttcgccttca ggacgcaaaa aggttactac ctgacggccg
     8641 tgaacggcgg aggacgaacc ggtgatccga ccatcatcac cggcgccacg tcggccggag
     8701 cctgggaaaa gtttagaatc ggcatcgcga ccccgccttc tccgtacgac agactcctcc
     8761 aaaccgcgac cggcaactat ctcaccgccg tcaacggcgg cggcatgacg gccaatgtcc
     8821 tgcacactga tgcgacacag cccaaagagt gggagcgatt ccgtctggtc tacatgggcg
     8881 gctacccgcc tccctactac ggcatcggga cgagcaaggg aaacttcctc actgccgtag
     8941 gggccggggg gcgctatcaa gatgcgatcc acagcgatgc caggcaagtc caagcctggg
     9001 aacagtttcg catcgtgaag tgcggtgatc cgggatcggg ctatgagtac ggcatcatgg
     9061 ccgccgacag cgaattgttc gcggcgtcca cctatcaagg caagacctgg tttggcctga
     9121 atggtcatcc cagtgaagcc gaaatgaaac tgaaggtgat ccgtcaaggc gatggtacct
     9181 acgcgcttca gacgacgagc ggacgttatg tcacggcact gggcggaggc ggacaagtcg
     9241 aaaagtacga ccggaaatac tggacccctg attgcggcgg cctcttcggc ggcccttgtg
     9301 tcggaggcgc ttcgcccatt tttcacatcg atgcgaccca ggtgcaggcc tgggaaaagt
     9361 ttcgcctcgt cgatcagggg aactgcacgt atacgattca aaccaccagc ggtttttttg
     9421 ccggcatttt caaagacgga tccggcgtaa cgttgctgac cacgagacgg gacggagcgc
     9481 cgacggccaa tgagaaattc caactcgtcg tcttcggcct tgcgtcaccg cccattattc
     9541 aatagcgcac atgcagaacg agacccatta cggagctgac gtagctcccg tagaatcgcg
     9601 agcccatcac ctatcaccaa ggaggcctct atgagatcgg atcgagtggt attcatcggc
     9661 gcaatcagtc tactgacatt cgctgtgatt cttcctgctc ccgcatctgc aaggcccaaa
     9721 ggcacctggc agagctgtaa taccaaggat ctcaacacca acttcggcgc ctcctgtaac
     9781 gatcagatgc aacaggacat catgggcaat aaaccctaca cccatgtcct cttttgcggt
     9841 ggagagacca tgctctgttg cacggtcgac aacaatacca atcaggtgat caactgccgg
     9901 aaaccggccg gcagccgcgt gatgccgggg ttgcagggca ataccctcgg cgcatccggt
     9961 atggctggcg tgcagagacg aggggtcgaa tcgaaggcac aagattcgga cgaggacgcg
    10021 ccggtgcctt caaccctcac gtcggatgtc gtgaaggaat tgcgccccaa ggccgccgca
    10081 aagtgacccc tcgttgttga gcagccgctg gaatgccgtt gacgggacgg agtgagaaat
    10141 tctcactccg tcccgcacac ctttcgggcg gtcgcgtcaa aacctgcagg cggccccccc
    10201 ttctctgatg ttgagacaat tatcgaggca ggtacttcac tacgtagtcg gtgccgtgga
    10261 aggaggcttc aatcacacgg atctgggcgg gactcatcgg attgataccg gcttggatga
    10321 gaaggtcgct ccggagggat tgccagacaa tctgatcgaa ctgcgtacgg gtctgcggat
    10381 ggctcaaggc ctcacgcacc cctgtttcaa tcgcattgct gagcgtatcc aggtattttt
    10441 tcgacacctc acagatgttg atggggaaga tcacatcgaa ccagtaggtg cacttgtcct
    10501 gcggcttgag cgtcccccga aaccccgccc gtaccgcctg gtaggtcggg aatccctgct
    10561 gattggtcgt gggtaaaatg aagatgtcga taattgcatt ctcgccgacc gtatcgccga
    10621 ccgcctggtc gccttccgga gaatagtccc gataataggt tttgaactgc agggctggaa
    10681 gatgaaactc cagatgcaat tcattgcctt ccgacttcac caccggcggg ttggacaggc
    10741 cgttcgcgtg cacaaacacc cgggagccgt ttgaggcttc gtgcacatag ggcggaacgg
    10801 tgaaccgacc agccaggctg cggccgatcg taaagttcga atcgacctgc ccgcgaaggt
    10861 tggtgagctg aacaaaagat ttagacaggt ctccgcccag caggagggcc aggggaatcg
    10921 tcttgaccaa aggcgacgtc acctgtggcg gctgcatcat cggctgcaga ccgggaccgg
    10981 gcatcaccgg ttgctgttgc cgtggccccg gcatnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnncccggc atcggctggc ccatcgctgg aggagctggc tgcgccggca
    11161 cagactgggt gtgagagcgg ggtggaaaga cctgggcttc tgcctgcggg acgaccccat
    11221 gcagcgtcat cagcaaagcg agcaaccaca accaggtctg acacctgaac ggcaggaagg
    11281 tgatcattcg ttccgagaag aggttcgcat cctgtgttgt tcaacaatcg tcgtctgttc
    11341 gctcatgacc ggcttccagg aactacatcg acatggggaa ttcgcaggat gctcaacgtg
    11401 gttgtccagc aacgccgcag ggatggaagc ggcgaggcgt acatcgcctg gtacgtcgag
    11461 tcgctgacag aaccgaggac ccagctggcg gccatgttca gcatcctgct actggtggcg
    11521 gaaccagcgt gtcacccgca catcatcgcc gaccttcgcc gcgacatagg acagtggcgt
    11581 cacctgcata gcctgctgcc cgtcgtccag cacctgagcc gcctgtcccg actgggtttt
    11641 gccgaatccg ttcgggatga cggccggctc ctcaacctgc gcggcgacct gaccggccgg
    11701 acgttgttga ccggccagtt gccctagact ctcctccaga tccgtccgtc gcgcaggaga
    11761 ggcgacgagg aagaaggttt cgacgccctt aaactgatcg agcgtgaacc aatagggtcc
    11821 gtcgggaaag gcctgttcca cttccggctt gagtggatta ttcaccgggc cgttggccac
    11881 agggaaaact gactgtgccc acccggaccc atcgatagag atgacgtaca cgaaacactc
    11941 gcagttcgtc cgcaccacca gcttgaagcg gtcgccatcc tgcccaccct tcagcgtctc
    12001 accgtcctgc atgagggcaa cctgtttgcc attcgatcca ttgacctgac ggatcagcgc
    12061 aacgtccaac gccaccggtt cagccggtcc cgggatacct ctcggataca cgccggctgg
    12121 atacgacacg ccgacattgg ggggctgccc atagggctgc gccatgccgg gctgggcata
    12181 gccttgcacc ggctgctgat acccttgtac cgccggttgc tgatagccgc ccatcgccgc
    12241 ctgctgcatc ggttgctgct gatacccttg catggcttgt tgctgataga gatccatcgg
    12301 ctgttgatac atccccggct gcatcggttg ctgaccgtac ccctgcatgc cttgtggagg
    12361 gtagccggga gccggggcct gcgcagccat gccgggcgcg gcggaaggat agccaggttg
    12421 cggcggcaat ccctgctgag ccggatagcc gggctgaggc gggtatccgc ccatctgccc
    12481 gccatacgac tgcgcaccat actggccgcc gtagggttgg ggcatgccat aggccggggc
    12541 ctgcacgttc atccccggta cggacgcgta gggattattg gggtcatagt accccccggc
    12601 ttgaccttga tagccatatt gcgacatgcc gtactgtgag gtgccatacc cgcccattcc
    12661 accataccca ctcataccgc cgtatccgcc catcccgccg tacccttgcg cgccataccc
    12721 gcccatcatg gcgccagccg gattgtaggg atccggatag cccattccca tgcccatccc
    12781 tgcctgaccc gatgcctgcg cgttcatcat cgcctgttgc tgctgcatca acatggtcgg
    12841 cgggactaac gccgcaggtg acgttcccat gggcaatccc atcgccacgt agggtgtgct
    12901 caggcgtacc atgagccggt tcatgtcttc tgagtatttc ccaccgtaat tcttgttgct
    12961 ggtgaccagc aggttgcgcg cgaattcaat gccggcggga atcagaaagg gtgcggccgc
    13021 gcaggaagcg agcaggagaa acgggaggaa aatgagcaaa cttgtgcggg tgcccgacgc
    13081 ggtccggagc ggctgtaggc gtgtatgcat gcgatcacct cgtacccgtc tgtcgcgcca
    13141 tctccggaaa ggttcacgat tgtagtctct catttatcga agggtcaacc ggcattgcta
    13201 ttgaggggat ccaggattct gtgctacagc agaagcgccg atcaggaccg ctggcacatt
    13261 tcttcgaaca tacaggctca tggacgcgac tcacgggcat aacgcactgg aacgtacgct
    13321 ggtggcgcga gtggcgaccg gagacacgca agcgttcgag gccctgtacc ggctctacga
    13381 caagcgcgtc tatcactaca tccagacctt catcagagat gccgctctgg ctgaagagat
    13441 cgtcggagaa gtgatgttcg cattatggcg aggcgcctgc accttcacgc accaatcccg
    13501 ggtttcgaca tggattttcg gcatcgcccg ccataaggcc ctcgacgcgg tcagacgcca
    13561 cggcaggatt gatcactcca ccgccccgct cgacgacgtg ccggatcttc cgagtcagca
    13621 ggaaactcct attgacggga tccaacgcga acaactcgga atcgtgaccc gccaggcgct
    13681 ctcaaaactg tcagccgaac atcaagaaat tttgcgcctg gtcttttatg aggaactgcc
    13741 ctacgaagag attgcgctgc tgctcagcat ccccgaaaat acggtcaaga aacgggtgtt
    13801 ttacgcgaaa caacagttga aacggcatat ggatcggatg ctctcgaggg agtctgtcgt
    13861 atgacgacgc ctcacaatct ggcccccccg cacccgacgg ccacattgct cccctggtac
    13921 ctgacgggga cattgaaaga atccgagcgg caggcagtcg atgaacatct ggcgagctgc
    13981 gccgactgtc gcgccgaact ggcccacctc acacacctgc gtacaccggt gaaaaccgcc
    14041 tttgcggaag agacgacgcc ggagctacgc gtgcagcaac aggtgatgat ccgcattcag
    14101 gccgaaacac tgccagctca ctcagcggga cccaccacgt ctaaagaagg tgccgccgaa
    14161 tcagtggaac agtggttccg ccgcctcttc gccccgcgct ggatccctgc gctggcggcg
    14221 atactcctga ttggccaact gaccctcctg ctgtggaccg ccggtgaaca gaccgccttg
    14281 ccgccaagcc acattgcgtc tcgcggaatc cagccggtgt ccagcaagat cgttctagtc
    14341 tttcaggact cgatcccgga ggcccgcatc cgcgcgacca tcctggcgtt gaaaggccga
    14401 ctcgtcgatg ggccaaccgt agacggacgt tacaccatcg agattccttc gacggaaacc
    14461 gctgccctcg accagcagct tcataccctg caacagcaat ccgctgtgat tcgacgtgcg
    14521 gagcgccttg cgccatgaac ctttttaaag ggtgcgcgac tcacccattg cagcccgagg
    14581 gccttctatg acgcaccgtt tctccactcg caccattctg agtctcgccg tcatcaccct
    14641 gctcgtcgtt cagccggtgc cgagttttac gaccgagccg ccatcgtcgg gaaccaagcg
    14701 agccttgctg atcggcatca atcggtatca agtgctaccg accttgaacg gctcactcaa
    14761 cgatatcgac accatgcggc aggtgctcac cacacggtgg ggatttcagt ctcaacacat
    14821 cacgatgctc acggaccagg ctgccactcg atccggcatt ctcgctgcgc ttgaacgact
    14881 cgtgcaggac accggtccat cagacgtggt ctatgtccac tactcgggcc acggatccca
    14941 ggtcgaggac ctcaacggcg atgaaaccga ggatcatctc gatgaaaccc tcgtcccgca
    15001 cgacgggcgg actccgagcg tccccgatat caccgatgat gaactcgaag ccatcgtctc
    15061 caagctgaag gcccgcagca tcttgatcgt cctcgactcc tgccattccg gcacggcaac
    15121 gcgttccctc gacatccgga cgcgatcggt tccacaagat acccggatag aactctaccg
    15181 accgtccggg gttcacactc gtcaggttgt gccggtcacg gctgcccgct acgtcctcat
    15241 gaccggcgcc gccgccaacc aggaggccct cgatggcccg gtgggtggac gttaccacgg
    15301 gttcttctcc tattcgctgt ctcgcagttt gggatcggcc gggctatcag cctcgccgag
    15361 agagattttc ttcggcgtcg aacgggagat gaagcggatc caaactcact tcggccggat
    15421 ctcgatgccg gaaccccagt tggaggcgcc gccggcgctc atggacgcgc ccctgctgcc
    15481 catgccttcg gagactggca atgagactgg tacaaatgga acggcgcggt tgccctgggt
    15541 cgaggtgaag gctcacaagg gaggcgagac cctcctgttg aacggtctcc agctgggtgc
    15601 tgcaccggga tccacttggg ccgtatatcc accgaacgaa accaatttcg caccgggaaa
    15661 atcgctcggg ctactcacgg tgacacaatc ccgcgggaaa gacgccgtta ccgcaccgca
    15721 cgcgacagcc ttcaccatcc cgaccggctc acgcgccatc gaatttctgc cgccggcctc
    15781 cgtagagggc gtgcccgttc gtctgttgga aattccaaat gaccgccgtg aaaaaattga
    15841 gcaagtcctg cgtgaacagg gacgtgaggt ccagatcgtc gggccgggcc agcctgcgca
    15901 atttctggtg gaggtgaaag gcaatcggtt gcagttgtat tcggccgacg ggcttcaact
    15961 cgtcagctcg ttctcactgg acaacgcaca gtggggcgcc ggactggcac gggtcctctc
    16021 ccgatcagcg aacgcctccg aactcctgac gctcgacaat ccgtcggcac aaatcaaact
    16081 ggacgtgcgg attgccgggg cagcacgccg tcaagcgcgc ggcattgccg tcgtcgccga
    16141 cacacagccc gcacagtatc gcatccggca tgaaggggaa ccccgcacac caggcaacag
    16201 cctgcagttg gaaatccagg cgaccgctga cagttatctg accattgtcg acgtggattc
    16261 ggaaggtcat ctgaatctgt tgtttcccac agtgcaccag cagccatcat tctacccgga
    16321 aggccgggtg cgggccggtg acgtggtcac cattcccgac tcgttacagt cgggcaacca
    16381 tgccggattc cattgggact atgcgccgcc ggccggcact gatacggtgc gtgtgtttgc
    16441 gtcgacagat ctcgagaccg cgcaggccat ccgccaacgc gtcatagcca ccactccccc
    16501 cggccctctc gccacaacga tggtgaccac tcgatcgatt ctctcgtcta gcatgacaca
    16561 ccttcggcag gacttgagtc gaatcgcgac gcggggactt tcatccgtct atgatccgac
    16621 tccagcggcg ttgacgaacc aaccttcgac gccgccaacc cagacgccaa cggctccgac
    16681 ggctggacct tcctcgcctc agccgcctgt gagtcaaaac ctctcaccac ccatcgctcc
    16741 atccaccgcg cagacatccg ccctcacacc ggcggcagac tgggccgcca cctcgttgac
    16801 tatagaggtc gcaccttaaa tcctgcctct tcgatacgtt tcaatggatt gatggatggg
    16861 aactcttact cacagaagag agtagcgagt tctcctgttt ttcgctgcgt cactagcact
    16921 ctgatctttg aggatttggg aggagaccca cggcgtcgtt caaatcgata tggaagcgag
    16981 ccggctcaat gtgaggctgc tgctggaaac cgcagagcat tcaagatcgc ggcaagacga
    17041 tagccagcct tggctaactg ctgctccaca attttcgcct catcttgttc gtagccccgg
    17101 ggcagggtga tcgtaatctt ccgcagacca gtatggtctc cgtcgaattg cgccgtgccg
    17161 aacacgaggc gctcataagc cgactttcct agcctgatgc cctcaaccgc ccactgatca
    17221 acccagtgat gatacgagcc gtcgccgtcc caggctcctt gatggccttg tgccagaaga
    17281 tcctgagcga gttccagata cgaccggcct ttggctacct cgttcaccag acggtcatcc
    17341 cagaaggcgt gtagctgctc ttcggctgca gttaataaca gggtgccgcc acggtcttcg
    17401 tccttccccg ccgcttcatg gggatcggtg atgaggtggg cctgacgttg agcatcgaac
    17461 tgataataac cactgccgag gtggagcggc tggtgaaggt cgccgagtaa atgaaccagc
    17521 cagcgcaagg cctgcgccct ggtcatctcg tgatcagaaa ccgtctgtga ttcaatgaca
    17581 cggatgcagc gctgaatcgt ctgcacaatg tcgttgaggc ggcttccgac cgctccttcc
    17641 gtatagacgg cggtatccag cggaagatcg acataatgcc attcccgatt gtcggggaag
    17701 tcttcgttga aacgctgagc ctcggcgtca tgggccaatg gcccgagatt tttcttggcg
    17761 cgtttcacct cgtccggcca ggttgcgacc gtcgccaagg tttcgcccga acgaaggagg
    17821 gtccaaactg ccgccctcgt ttcaggtgtg agctgggcat cggcaatggc tccgaccgtt
    17881 cgatggccca gatctccaaa ggcccaggcc tgagcggtaa aggaccagcc cgcgatcgtc
    17941 aaaagaagga tgagaagtgc tgacatacca agccctcgct tcgagccggg atgaaagccg
    18001 tgacaggaac gatctgttgt gcgacaggga ggattgtaga agttaccgag tacaagcgca
    18061 aacatcagcc cgccccaact gtcgttttga gctgaatgct gacgcaacac agatcggaca
    18121 ccaatttatg acaaataaaa agggggaagc ctttcggctt cccccttggt gattaggacc
    18181 ggcactgcat ccttacggcg gccccttcga tagaagaggc cgccgccccc tcaccttatt
    18241 ggatgagatg tgaagtcttc gaggcgtgcg cctcgccgga ctcagatccc gcccgcttga
    18301 cagaaggtgc ctgctgactc aacgggagga ttcgttggtc atcggtgggc aacaccttca
    18361 agagacccca ttgacccgat tgggcatagg gcaagcgctg attgctccac acataatcgc
    18421 ccggctgacg gaagcttccg cctgccgctt ggatgaaggc atccagcgat tctgacgccg
    18481 agaactccac gacgctgatc tggtcggcgc cgcgcatgaa cggctcgatc ggccattcat
    18541 gtttttccac actgaacatg ccgttctgtt cattgctggc accgaagaca tggatgcgca
    18601 cgggatcgcc cgcatgcgct tcgatgatcg gagtggcaat gctttccggt ttgtccaccg
    18661 agcagggctg gaacaccttg ccgagcgagc agccgctggc ttcacggaac ttatacggtt
    18721 ccgcacggta gttcacggcc gtcaaacccg ccgtgttttg cacgtagggc atgaagctcg
    18781 ttccaatgat gttgtcctca tcctggaaga acagggccac atcgcgatag ttctggcgag
    18841 tttcgtagcc ctggatcgtc cgatccacaa tgacatcggc aatccagctg ttcttggtgg
    18901 agatatccgc accggtcttc ggatcgcgat aggtcgc
//
LOCUS       DAOQEW010000062        18906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_746_length_18906_cov_9.369370, whole genome shotgun sequence.
ACCESSION   DAOQEW010000062 DAOQEW010000000
VERSION     DAOQEW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_746_length_18906_cov_9.369370"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="atpD"
                     /locus_tag="PLS35_08655"
     CDS             <1..962
                     /gene="atpD"
                     /locus_tag="PLS35_08655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNI18771.1"
                     /translation="VGKTVIIMELINNIALHHGGFSVFAGVGERTREGNDLWHEMQES
                     KVIDPDDHTKSKAALVYGQMNEPPGARLRVALTGLAVAEFFRDEENQDVLLFVDNIFR
                     FTQAGSEVSALLGRMPSAVGYQPNLSTEMGALQERITSTKKGSITSVQAIYVPADDLT
                     DPAPATAFAHLDATTVLSRQLAELGIYPAVDPLDSTSRILDPQVIGEEHYKVARGVQS
                     VLQRYKDLQDIIAILGMDELSEDDKMVVARARKIQRFLSQPFHVAEAFTGAPGKYVKL
                     KDTVRSFKEILEGKYDHLPEQAFYMVGPIEEAVAKAEKMGVKV"
     gene            1076..1498
                     /locus_tag="PLS35_08660"
     CDS             1076..1498
                     /locus_tag="PLS35_08660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNI18772.1"
                     /translation="MAGKILLEVVTPEKLLLSQEVDEVIAPGSEGEFGVLPGHCHLLS
                     SLRIGELRYKSQEVWHYMSILWGYAEVTPVKVTVMAEIAEKAEDIDVGRAQQAVEKAE
                     QRLQAGGLPSEVREAEISLEKARLRKKIADRARKTGHA"
     gene            1662..2453
                     /locus_tag="PLS35_08665"
     CDS             1662..2453
                     /locus_tag="PLS35_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18773.1"
                     /translation="MQQRSDWFVRLDSVQSTEPSPVVYDHPFSWKAEELSAILSRLFL
                     EERVGLMDNAKPAKPVFTPVEIDLVEPAVREAFGKARPGEWIAFLLLEPNAGATGVTS
                     GGLFLESHRLHVVVANHRTMVGNNSDELVRVRANPFYSIQGSGGVLAFEPSRFRLSTK
                     ANWSGGHRASASELVLDHSAFLSYVTHAAPREMPTSAPSPIRQQSSAEALTSPADASV
                     QGGPHDQPMLPQIQGEIERLKQELAEKDLEIDRLKAQLLKSGARP"
     gene            2609..3541
                     /locus_tag="PLS35_08670"
     CDS             2609..3541
                     /locus_tag="PLS35_08670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNI18774.1"
                     /translation="MQTEFLISPGEQPKRLDIFLVNREPKLSRAAVQRMISAGRVRVN
                     NQTAKPSQKIKPGDVVNLDKPKPTPLLINGQPSALEILFEDSACLVINKPAGVAAHPG
                     PGHWDDTLLNALLDHALRAGVVGMSGLVHRLDRDTSGVMVVAKTKTAHGRLAVQFEQH
                     SITRQYEALVQGVPQHSHGRIELALGTDRNNVKRTSPHTLHPKASLTEYYVEEVFGAA
                     AAHMVLTPRTGRTHQIRAHLHAIGHPILGDPAYGAEPVRALEGYGVPRVMLHAHKLGF
                     HHPLTERYCEFTVKPPADFRGLSDELRFKTERQG"
     gene            complement(3605..4090)
                     /locus_tag="PLS35_08675"
     CDS             complement(3605..4090)
                     /locus_tag="PLS35_08675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18775.1"
                     /translation="MSSNFIHRILRSLRSSVDPTPSVEPLVVDNITDLPLTEVLRERR
                     LDARFAVRTPCLYELDRKQRRETPVISGKAHSLNVSSGGVLLLLDQQPQTGQVLSLHN
                     PTLHSQPGTSLFEVRWTTSLPLGTQQGCYLVGCHLAFGRFPFFLVQRQYLHRDISSLS
                     I"
     gene            4472..5455
                     /locus_tag="PLS35_08680"
     CDS             4472..5455
                     /locus_tag="PLS35_08680"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNI18776.1"
                     /translation="MNRVSTTPVEIVVTGGESSKRIDVFLANRDPTFSRSALQRLIGE
                     GRIQINGQPVRPSQKIRPGDRIRLEVPRPEPLDLQPEAIPLEILHEDSDLLVLNKQAG
                     LVVHPAPGNWSGTMVNALLHHFATAGVTPSHIGGKERPGLVHRLDKETSGVMVIAKTD
                     QAHRALAAQFKLHTITRVYEALIWGVPKKGHGLIELAIGRDTKERKKFSARTTNPKAS
                     ATDYQVEERYGKIAAQVRLSPRTGRTHQLRVHLTSIDHPILGDKTYGGAKVMTVAGIP
                     IPRVMLHARTLGFTHPTGGEYHEFDVPFPPDMEQVQHLLRDATTQGGGCRQ"
     gene            5518..5940
                     /locus_tag="PLS35_08685"
     CDS             5518..5940
                     /locus_tag="PLS35_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18777.1"
                     /translation="METSAVLDRIGELIAQLKGYAAKLPTVVHLASVPTGVKPKPEAV
                     EAFEQALLRFREQSGRSPFKGLQEYLLESLEAFEVGNVLGSVQPLMAVLDQVERMQRD
                     KEIKVTPAEDKRMGEYRAALIKILPGNQPELEGAGRGM"
     gene            complement(6029..6208)
                     /locus_tag="PLS35_08690"
     CDS             complement(6029..6208)
                     /locus_tag="PLS35_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18778.1"
                     /translation="MGTQVRSCPKCFQLMWLKQDHYDVIDEETVRTKCPHCDSTVRFQ
                     LVTAGANAAGPKMGH"
     gene            6272..6712
                     /locus_tag="PLS35_08695"
     CDS             6272..6712
                     /locus_tag="PLS35_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI18779.1"
                     /translation="MPPALKTGQRPIAFSDRNDFDATQLIHLYRQAPWAKHRALEQAQ
                     AMLAKTDLVISAWDGPRLVGFGRVLTDYIFRASIWDVIVDRDYQGQQIGTEIVRRILD
                     HPTLQQVELFWLCTRMPGFYERLGFSAKEQTGMVWSRKQPSTQS"
     gene            complement(6812..7633)
                     /locus_tag="PLS35_08700"
     CDS             complement(6812..7633)
                     /locus_tag="PLS35_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNI18780.1"
                     /translation="MLELLAYDFMQRSLLAAALVGSVCSVIGVFVVLRGLAFAGAGTA
                     HAAFAGVTLAYLLGLPPLSLAIVFGLATVWITGWVEEKGRMKLDVSIGILYTATMALA
                     ILFLGLMKTYNPEVYGYLFGSVLSVTTEELLTIGGLSIAVLGTILLLSKELYFIAFDQ
                     EMAAASGVPARQIFYLLLTLVALTVVIALKTVGAILVFAMILIPASTAYQLTHSLTQM
                     TLYSMLIGIFCAVTGVLLSYAFDLPSGPSIVLLATSLFFLAVCCSPKRLHRIQAE"
     gene            complement(7720..8502)
                     /locus_tag="PLS35_08705"
     CDS             complement(7720..8502)
                     /locus_tag="PLS35_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNI18781.1"
                     /translation="MTHPIIRFDHATFGFPGTVALEDISLSIPESEFVGVIGPNGSGK
                     TTLCRAVLGLMAPIRGTLRVLDCACEELRCHHRALIGYLPQKGMLDRNFPVTVLEAVM
                     MGRYGALGLFRRPSGKDRDIARQALTQVGMDHHRDSALGALSGGQQQRVFIARALAQQ
                     PRILLLDEPTTGLDLTAQHSVVELIQQLHQELKLTILMITHDINMIRSRVDRLVLLKT
                     KLFAAGPPQDVLKPEILSQVYGKELVITDKDFIIVEDYHHHH"
     gene            complement(8560..9480)
                     /locus_tag="PLS35_08710"
     CDS             complement(8560..9480)
                     /locus_tag="PLS35_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNI18782.1"
                     /translation="MFRTILLTLFIFLTMLGNLSAAVPTASAAEPLNVVVTLPVLKDW
                     VQQIGGSHVHVVSLMTGYESEHTYSPKPSDLVAVRKAALLFEVGAGLEVWVSSLVKNA
                     GNASLQVVTTSKDIELIPDHPESTADAHTHHHAGGNPHVWLDPSAAATMVQHISKAMV
                     EADPAHAAEYRNNTADYLQALLRVQAESLERLKKVPSRAVIVHHPAWPYFARRYDLQI
                     AGTILTQPGGEPSARHLHTLIDAIKRGHIRVIISEVQLNQKVPQLLARETGAHIAVLT
                     TLPGGLPGTETYLDMLRYNVLQLAHALEQT"
     gene            9642..10826
                     /locus_tag="PLS35_08715"
     CDS             9642..10826
                     /locus_tag="PLS35_08715"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNI18783.1"
                     /translation="MKVVGLMSGTSADGIDAALVNIVRRGGRPVITPIAFSTVPYPRA
                     LQQRVVDLSLHGQVAEICHMNAYLGELFAEAALKVIRAAKHQPADIHAIGSHGQTIHH
                     LPKGRRESGVGLIRSTLQIGEPAVIAERTGITTVANFRPRDMAAGGEGAPLVPYAHAV
                     AFSHARRTRLVVNIGGISNVTYLPAGGVMADVRAFDTGPGNMVLDAIMQETTKGNRTY
                     DAGGRLARKGTIHQPLLTELMAHPFLTRRPPKSTGREEFGAPLVRRLLAKQKKARLSI
                     EDLLATCAAWTAEAIGSSRRWVPGKIDDVIVGGGGVSNRAIMASLRTVFAPAPVLTFD
                     ECGWSSKAFEATAFALLAHDVLHGHCTNVPQVTGARHPVLLGSVVPGKADIRRRNRYL
                     PG"
     gene            10836..11360
                     /locus_tag="PLS35_08720"
     CDS             10836..11360
                     /locus_tag="PLS35_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNI18784.1"
                     /translation="MNMDVLYPLLAIGALVLLGSLAWETFGRRRAPAASSGSLPSGLT
                     FAPKPLLTDQEVQLYNLLRLAVEDRYLLFTQIPLWSVLDLRVPSGMPPAETLRELALT
                     RATFVLVHPGSRLVEKVVQVEPSASQAEGQGTANSLFDGALRAAGVQLVRLSPFHTYT
                     VPGLVTVLDLADPD"
     gene            complement(11425..11946)
                     /gene="ybgF"
                     /locus_tag="PLS35_08725"
     CDS             complement(11425..11946)
                     /gene="ybgF"
                     /locus_tag="PLS35_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNI18785.1"
                     /translation="MTMVQLSREKVRGIAAALALFSILASASDLFAASPQQPDASRRL
                     YDRVMDEFRHKDYPAALAGFRFFLELHGHSSLSANAQYWMGECQYRMGKHDEALGSFF
                     KLISDYPMSQKLAASTLKIGQIYTKQGDLEKAQMMYERVTGEYPDSPEAEVARKAIEA
                     AAAKGDPIAAGPN"
     gene            12255..13088
                     /locus_tag="PLS35_08730"
     CDS             12255..13088
                     /locus_tag="PLS35_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNI18786.1"
                     /translation="MDTSHEAGSSGAGKDPLIEPPPNPKGGKTLIVDARDPDAYLCPS
                     AALKDAGEDDQVFVRPGVYEDKVFVADKPVLLVGAGRDQVQIYSRRGGPLYLQRVPNG
                     MISGITFRYVGSDQHSAMNVLDSVCTITRCRAMEGILSGVVIYGPEARPTFIDNEVCS
                     NRESGIFVFAGAQPRVAQNLCYGNHHFGLAVRDPGSHPECVRNVCHSNMLSGMLLFHH
                     AEALLLDNICRDNQHWGLVMTPDSHPTPSQEELVAGNVFLPNPRGSVVVTEQPLADIG
                     R"
     gene            complement(13150..14079)
                     /locus_tag="PLS35_08735"
     CDS             complement(13150..14079)
                     /locus_tag="PLS35_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HNI18787.1"
                     /translation="MIKLLLVEDNSVDAQLTQDLLAEWSLDRFEITHASVLADGLTRL
                     SRERFDVVLLDLSLPDTHGLATVTQVLATSPDVPVVVLSGHDDHPLALQALQHGAQDY
                     LVKGEGGTEFLARSILYAIERKKAQERLTYFAQHDPLTGLINRALFRDRLIHAMARSK
                     RKDQPLAVMLLDLDRFKAVNDTLGHDIGDQLLKEVATRLMECVREVDTVARLGGDEFT
                     AILEGISGEDDVLVVAKRIVDSIGSPFQIGPHNISIGVSIGITLYPSDDEDLDELLRH
                     ADKAMYAAKQQGGGRYHFHSPAGHSPDVPAPLP"
     gene            14591..15367
                     /locus_tag="PLS35_08740"
     CDS             14591..15367
                     /locus_tag="PLS35_08740"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNI18788.1"
                     /translation="MAQEQSARDQESFSRTSSVDDCLDGKTRTSVSWALPLAIAGVLV
                     VGDSASAENDIYGYVGANGVFEMTNVPTDGRFRPAELQRSRLSHRASVEEVEDAVERY
                     AWQFHLHPALLLAVIKAESDFNPTVISRAGAVGLMQLIPETAIRHGVHNLYDTGDNIR
                     GGARHLRYLLDRFNGNIRLAVAAYNAGERRVERYHAIPPYPETRDYVRKVMIYYKSFR
                     GDYQATPGKAVLLAMHHKRLQLEPLSVPVDFRHGRPAAKK"
     gene            15965..16822
                     /locus_tag="PLS35_08745"
     CDS             15965..16822
                     /locus_tag="PLS35_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI18789.1"
                     /translation="MPDTPFSESRSGPPSHGHLSLRSLLISALAWPFKTFIRRLIGEV
                     FYQQARVTLLHEHGGVGLDWRWGNSLRRVHPIRRDFGWQSGRPIDRYYTEDHFLPAYQ
                     ADIRGRVLEIGDDRYTRQFGGERVTLSEILNVNPGMGGTTIQADLTDAKQIPSNTYDC
                     AILTFTLQFIFDVPAALRTLGRILRPGGVLLVIVPGISQIARYDMDNWGEFWRFTSLS
                     ARRLFEQVFRPEDITITTYGNVLSSLASLHGLHSTELTTAELDHWDRDYELVIGIRAV
                     KRPAANTSL"
     gene            complement(16961..17665)
                     /locus_tag="PLS35_08750"
     CDS             complement(16961..17665)
                     /locus_tag="PLS35_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNI18790.1"
                     /translation="MHSALTTPTEITASTPRHQCLIYKGSPATHFSGLSAVIRQKLTE
                     NYRCLYFNTSAMIAAMRPYLLAAGIDIPQEVERGRLVLSSNPTHLTDGHFSIDRMLGM
                     LEEGLNQALQDGHAGFWCTGDMTLEFGPERDFSKLLDYEWRLETFLQSHPALSGICQY
                     HADTLPNEVLRQGLLAHPSLYINETLSRLNPHYVDRESFTAATYHDPALDKTIRALCM
                     VPDALILNALPPGYLH"
     gene            18092..18328
                     /locus_tag="PLS35_08755"
     CDS             18092..18328
                     /locus_tag="PLS35_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HNI18791.1"
                     /translation="MRQTIKARYHDGVLQPLEPLSLADDAEVQVTVDTDLALGTDEIL
                     RRAAQVYQGLSADEITQVESIALDRQHFFREPAA"
     gene            18328..18744
                     /locus_tag="PLS35_08760"
     CDS             18328..18744
                     /locus_tag="PLS35_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNI18792.1"
                     /translation="MPQPAVLLDTDILSELLKQHPLVVQRVRSYLTEHQRLAFSIITR
                     YELLRGLKAKQARTQEAAFTLLCQASLILPITDQVVERAATLYGDLHRQGALLPDADL
                     LIAATALDAQRPLITNNLAHFQRIPGLIVESWKRSV"
BASE COUNT         4118 a         5174 c         5572 g         4042 t
ORIGIN      
        1 gagtcggcaa gaccgtcatc atcatggagc tcatcaacaa catcgcgctc caccacggtg
       61 gattctcggt gtttgccggc gtcggtgagc gtactcgaga aggcaacgac ctttggcacg
      121 aaatgcaaga atccaaggtc atcgatcccg atgaccacac caagtcgaaa gcggccttgg
      181 tgtacggaca gatgaatgaa ccgccgggag cgcgtctccg tgtggcgttg accggactgg
      241 ccgtagccga atttttccgt gacgaagaga atcaggatgt gttgttgttc gtggataaca
      301 tcttccggtt tacccaagcc ggttctgagg tgtccgcgtt gctgggtcgt atgccgtcag
      361 cggtgggtta tcaaccgaac ctgtccactg aaatgggcgc ccttcaagag cgtattacct
      421 cgacgaaaaa agggtccatc acgtcggtgc aagccattta cgtcccggcc gacgacttga
      481 ccgacccggc gccagccacg gccttcgcgc acttggacgc caccacggtg ttgtccaggc
      541 agctggccga gttgggcatt taccctgcgg tcgacccgct cgattcgaca tcacgtattt
      601 tggatccaca agtgatcggc gaagagcatt acaaggtggc gcgtggtgtg cagtccgtgt
      661 tgcagcggta taaggatttg caggacatta ttgccattct cggaatggac gaactgtccg
      721 aagacgacaa gatggtcgtg gcgcgtgcgc gaaagattca gcggttcctg tcgcagccgt
      781 tccatgtggc cgaagccttc acgggcgcac ccgggaaata tgtgaagctg aaggacacgg
      841 tccggagctt caaggagatc ctggaaggga aatacgatca ccttcccgaa caggccttct
      901 acatggtcgg tccaattgaa gaagcggtcg cgaaggctga aaagatggga gtgaaagtct
      961 agcagccagg tgcccttggg cttgcaggcc gcgcacattc agaaggtgcc cggccgatgc
     1021 gcggacgaca gggcatcctg gtgttagcct tttgcgagag agcaggaaaa ggacaatggc
     1081 tggaaagatt ctcctagaag ttgtgacgcc ggaaaagttg ctcctgagcc aggaggttga
     1141 tgaggtcatc gccccaggca gtgaggggga atttggtgtc ttgcctggcc attgtcatct
     1201 gctgtcgagc ctccgtattg gagagcttcg atataaatct caagaggtgt ggcactacat
     1261 gtcgatcctc tgggggtatg ccgaagtgac gccggtgaag gtcaccgtga tggcggaaat
     1321 tgccgaaaag gccgaagata tcgatgtcgg ccgtgcgcaa caggctgtcg aaaaagccga
     1381 gcaacgctta caggccggcg gtctcccgtc agaagtgagg gaagcggaaa tcagcctcga
     1441 aaaagctcgc cttcgcaaaa agatcgccga ccgtgcgcga aaaacaggac acgcttaagt
     1501 tctcgcctgg aacttcttct cgttccgtat ctgccgttct tccttatcga gctcgtctag
     1561 gtcctcaagt actgcctagt agactctctc gctatgcctg gttcttcctt ttcctattga
     1621 tatgctccgg ctgtgccggc cctgtgctgc tgagtcgaga cgtgcaacag cggtccgact
     1681 ggtttgttcg cctcgattcg gttcagtcca ctgaaccatc tccagtagtc tatgatcatc
     1741 cattttcatg gaaggccgaa gagctctccg ccatcctatc caggctcttt ctcgaagagc
     1801 gtgtgggctt aatggataat gcaaaacccg caaagccagt atttacgccg gtagaaattg
     1861 acctcgtcga gccggctgtt cgagaagcct ttgggaaggc gcgcccaggg gagtggatcg
     1921 cattccttct cttggagccg aacgccggcg caaccggagt aacatcaggc ggtctctttc
     1981 tcgaatctca taggttgcat gtcgtggtgg ccaaccaccg aacgatggtc ggaaacaatt
     2041 cggatgaatt ggtacgagtg cgagctaacc cgttttactc cattcaaggg agcggcggag
     2101 tactcgcatt cgaaccgtcg aggtttagac tctccactaa agccaactgg tctggtggtc
     2161 accgggcttc agccagcgaa cttgtcctcg accattcagc attcctctcc tatgtcacgc
     2221 acgcggcacc acgcgagatg cccaccagcg ctccgtcacc aatcagacag cagagctcag
     2281 cggaggcgct cacgtctccc gctgatgcat cagtccaggg cggccctcac gaccagccca
     2341 tgctgcctca gattcaggga gaaatcgaac ggctgaagca ggaactggcg gagaaagacc
     2401 tggaaatcga tcgtctcaag gcccaattac tcaagtccgg agccagaccc tgatcgagtt
     2461 tcgtgtcgat actactgcca ttcacgttgt caggctattc tctgtccgct aagggtttct
     2521 cggcgaaccc ccaaaccttc agagcgatta ctgctcggtg ttctggccaa ttgcaatcgt
     2581 ccgccatgct gccgtagcct agcgggcgat gcagacagaa tttctcattt cgccgggcga
     2641 acaaccgaaa cggctggata tctttctggt caaccgtgag ccgaaactct ctcgtgcggc
     2701 ggtgcagcgg atgatttcag ccggacgggt gcgcgtgaac aatcagacgg ctaagcccag
     2761 tcagaaaata aagccgggcg atgtggtcaa cctcgacaaa cccaagccga cccccttact
     2821 gatcaatggg cagccgtcag cactagagat tctgttcgaa gattccgcct gcctcgtcat
     2881 aaacaaaccc gcaggagtcg cagcccatcc aggccccggt cattgggatg acaccctatt
     2941 gaatgcgctt ctcgatcatg ccttgcgggc gggagtcgtc ggtatgtcag gactcgtcca
     3001 tcggttagac agagacacct ctggcgtcat ggtcgtggcg aaaacaaaga ccgcgcatgg
     3061 acggcttgcg gtgcagtttg aacagcactc gatcacgaga caatacgaag ccctggtgca
     3121 aggcgtccct cagcactccc atggccgcat cgagcttgcg ctcggaaccg atcgtaacaa
     3181 cgtgaaacga acctctcccc acacgctgca tccaaaggcc tcactcactg agtattacgt
     3241 cgaggaggtg ttcggtgctg cggccgcgca tatggtgctt accccgcgga ccggccgcac
     3301 ccaccagata cgagcccatc ttcacgcgat tggtcaccct atcctgggag atccggccta
     3361 cggtgcagaa cctgtccgtg cgctggaagg atatggggtg ccgcgcgtaa tgctccacgc
     3421 gcacaagctg ggtttccatc atcctctgac agagcgttat tgcgaattta cggtgaagcc
     3481 accggccgat tttcgcggac tgagtgacga actacgtttc aagacagaga ggcaaggctg
     3541 aggccgaaca cagtgaatag cctcatcctc cggcgtgtgt gatgagggtg tatgaattcg
     3601 aaccctagat ggagaggctg gaaatatcgc gatgcagata ttgccgttgg acgaggaaaa
     3661 acggaaagcg tccaaaggcc aaatggcatc ccaccagata acatccttgc tgcgtgccga
     3721 gtggaagtga cgtcgtccat cggacctcga acagactggt tcccggttgt gagtgcaacg
     3781 tcggattatg aaggctcagg acctgcccgg tttgtggttg ttgatcgagc aacaaaagca
     3841 cgccgccgga actgacattc aatgagtgag ccttgcccga aatcacggga gtttcacgcc
     3901 gctgcttcct gtccaattcg taaaggcagg gcgttctcac cgcgaatcga gcatcgagcc
     3961 gccgttcccg gaggacctca gtcagtggca ggtctgtgat gttgtccaca accagcggtt
     4021 cgactgacgg ggtgggatca accgaactgc gtagcgatct gaggattctg tggataaaat
     4081 ttgaggacat cagatggtcc ttctcgctaa aaggtgcgac gcgaaatcct tcttataaga
     4141 aagcaattca agtgcctcaa aacgagaaaa tattccgcac gccaaccgac tgatttgtgt
     4201 cggagtctgt ttcgcgcaag caccgggagt cataaattac gcgtcatggc cccgtcacat
     4261 ttgccgtccc cctgacaaag atgaacggcc tgacgatagg ccgctgccgg gacgtgccga
     4321 ggatcgaata gcacaaactt gtggatggct tgtggatggg gtgtgtgcga cttgtgaagc
     4381 aaggtggata tctcgacatc ttgtttttag cccctcccta tggcagctta cccccgctca
     4441 tgatgtcggt cacagatttg gcagttacca cgtgaataga gtcagcacga cgccggtgga
     4501 gattgttgtc accgggggag aatcgtcaaa acggatcgac gtctttcttg ccaatcgtga
     4561 cccgaccttt tcccgctcgg ccttgcagcg gctgattggg gagggccgca ttcaaatcaa
     4621 tggccaacca gtccggccga gtcaaaagat ccggccgggt gatcgaatca gactggaagt
     4681 gccgcgcccg gagcctcttg atctgcagcc tgaggcgatc cctctggaaa ttcttcacga
     4741 agactccgac ctcttggtcc tgaataaaca ggcgggcctt gtcgtgcatc cggcaccggg
     4801 gaattggtcg ggaacgatgg tgaacgcctt attgcatcac tttgccaccg cgggcgtcac
     4861 tccctctcac atcggcggaa aagagcggcc gggccttgtt caccggctcg ataaggaaac
     4921 gtccggggtc atggtcattg ccaagaccga tcaagcgcac cgtgcgctgg cggcgcaatt
     4981 caaattgcac acgatcaccc gcgtttatga agcgttgatt tggggagtgc cgaagaaagg
     5041 gcacggcctc atcgagctgg ctatcgggcg cgatacgaaa gagcgaaaga agttttcggc
     5101 ccgcaccact aaccccaaag cctccgccac ggactaccag gtggaagagc gatatgggaa
     5161 aattgccgca caggtgcggc tttcccccag gacagggcgc acccatcagc tgcgggtcca
     5221 cctcacctcg atcgatcatc ccatactggg cgataaaaca tatgggggag caaaagtcat
     5281 gacggtggcg ggcatcccga taccccgggt catgctgcat gcccgcacgc tgggattcac
     5341 acatcccacg ggcggggagt atcatgagtt cgatgtgccg tttcccccgg atatggagca
     5401 ggtgcagcat ctccttcgcg acgccacgac acaggggggg ggctgccggc agtgatgtgc
     5461 ggtcaacaaa acaggaggcc gacctttcaa gtcggcgagg gtgaggagaa gccaacgatg
     5521 gaaaccagtg ccgtgctcga tcggatcgga gagctgattg cgcagctcaa gggatatgcc
     5581 gccaagttac cgacggtcgt gcatctcgca tccgtgccga ccggcgtgaa acccaaaccg
     5641 gaggccgtgg aagcatttga gcaggcgctc ttgcgttttc gagagcagag tggccgatcg
     5701 ccattcaagg gattgcagga gtatctgctt gaatcgttgg aagcgttcga agtcggcaat
     5761 gtgctgggct cggtgcagcc gctgatggcg gtgcttgatc aggtggagcg gatgcaacgg
     5821 gataaggaaa ttaaggtcac cccggcggag gacaagcgga tgggcgagta ccgtgcggcg
     5881 ctgatcaaga ttctgccggg caatcagccg gagttagagg gtgccggacg gggcatgtag
     5941 ccctgcaatc agcagggcac attgatgatc tctgaaatga atcgccaatt tggaattcag
     6001 ccctcagaga gcaggactgg tcgagcggtt agtgtcccat tttcggtccc gcagcgttgg
     6061 cgccggcggt gaccaactga aagcggacgg tcgagtcgca gtgtggacat ttcgtgcgga
     6121 ccgtttcctc gtcgatcacg tcgtagtggt cctgcttgag ccacatcaat tgaaaacact
     6181 tcggacagct tcttacttgc gttcccatag gcttcctctt acactacggt taaccgatta
     6241 ctcccgttcg gagggattaa tttagtacaa gatgcctcct gctctcaaga ccggccaacg
     6301 acccatcgca ttttccgatc gcaacgattt cgacgcgacc caactgatcc atttgtaccg
     6361 gcaggctccc tgggccaagc atcgcgcgtt agagcaggcg caagctatgc tggcgaagac
     6421 cgatctcgtc atttcagcgt gggacggccc acgtctggta ggattcggtc gagtcctgac
     6481 ggactatatc tttcgcgctt caatctggga tgtgattgtc gatcgcgact atcaggggca
     6541 acagatcggc actgaaatcg tacggcgcat tctcgaccat cccacgctgc agcaggttga
     6601 attgttctgg ttgtgcacgc gcatgccggg attctacgaa cggctcggtt tcagcgcaaa
     6661 ggagcagacc ggcatggtct ggtctcgaaa gcaaccatcc actcagtcct aacagacccc
     6721 ctgtccttcg ctcccgtccc ttctacttat tggcacactg gcacgacgta ccagccaacg
     6781 gtgctgttcg gcaacagtct ggcgtttgtg tctattctgc ttgaatccga tgcagccgct
     6841 tcggtgaaca acagacggca aggaagaaga ggcttgtggc caataacaca atcgatggtc
     6901 cggacggcag gtcgaatgcg taggagagca acacgccggt gacggcacag aaaatgccga
     6961 tgagcatgga atacagcgtc atttgcgtga ggctgtgtgt gagctggtag gcggtggagg
     7021 ccgggatgag gatcatcgcg aagacgagaa ttgcgccgac cgtttttaag gcaatcacga
     7081 cggtcaacgc caccagtgtg aggaggagat aaaaaatctg tctcgcgggc acgcccgacg
     7141 ccgccgccat ttcctggtcg aaggcgatga agtagagttc ttttgaaagc agcagaatcg
     7201 tccccaacac cgcgatgctc aatccgccaa tggtcagcaa ctcctctgtg gtgacggaca
     7261 gcacactgcc gaagaggtac ccgtagactt cggggttata cgtcttcatg aggcccagaa
     7321 acaaaatagc caaggccatg gtcgcggtgt agaggatgcc gatggacaca tccaacttca
     7381 tgcgcccctt ttcctccacc cagccggtga tccacaccgt ggcgagaccg aacacaatgg
     7441 cgaggctgag aggcggcagg ccgagcagat aagccagcgt gacgccggca aatgcggcat
     7501 gggccgtccc tgcaccggcg aaagccaggc cccgcaatac tacaaagacg ccgatcactg
     7561 aacagaccga gcccaccagg gcggcggcca gcagtgagcg ttgcataaaa tcgtaggcga
     7621 gtagctctaa cattgtctca cgagatcagt gaaccatcgg ggaagacata acgaacacat
     7681 tgtatcgtga attcaggctc tatcatgaga tcggaatggt cagtggtgat gatggtagtc
     7741 ttccacgatg atgaagtctt tatccgtaat gaccaactct ttgccataga cctggctgag
     7801 aatctccggc ttcagcacgt cctgcggcgg gccggccgca aataatttcg ttttgagcag
     7861 gaccaatcga tccacccgcg agcgaatcat attgatgtcg tgggtaatca tgagaatcgt
     7921 cagtttcagt tcctgatgca gctgttggat cagttccacc acactgtgct gggccgtgag
     7981 gtccagcccg gtcgttggct catccaggag cagaatgcgc ggctgttgtg ccaaggcccg
     8041 tgcgatgaac acacgttgtt gctggccgcc ggacaaagca cccaacgcgg aatcccggtg
     8101 atgatccatg cctacctgcg tgagcgcctg gcgggcaatg tcacgatctt tcccggacgg
     8161 ccggcgaaac agacccagcg cgccatatcg ccccatcatc accgcttcca gtacggtgac
     8221 gggaaagttg cgatccagca tacccttttg cggaagatac ccgatgaggg cgcgatggtg
     8281 gcagcgcagt tcctcgcagg cacagtcgag gacgcggagc gtgccgcgaa tcggcgccat
     8341 caatccgagc acggcgcggc acagagtggt ttttcctgac ccgttcgggc cgatgacgcc
     8401 gacgaattcc gactcgggaa tggacagaga aatgtcttcc aaggccacgg tgccgggaaa
     8461 accgaaagtc gcatggtcaa aacggataat cggatgggtc atgcgtggcg cgttcgatga
     8521 agatgctggt tgtcgtcgat gtccggttgg aggcggcggt tatgtctgtt caagcgcgtg
     8581 ggccaattgg agcacattat agcggagcat gtcgaggtag gtctcggtcc ccgggagacc
     8641 gcccggcaag gtggtcagga cggcgatgtg cgccccggtt tcccgggcca gcagttgtgg
     8701 aactttctga ttcagttgaa cctcggaaat aatgacccgg atatgcccgc gtttgatggc
     8761 gtcgatcagc gtatgcagat gccgtgcgga gggttctcct ccaggctgag tgagaatcgt
     8821 tccagcaatc tgtagatcgt aacgccgggc aaaatagggc caagccggat gatgcacgat
     8881 gaccgcacgg ctcggcacct ttttgagccg ctccagcgat tctgcctgaa cacgcagcag
     8941 ggcttggaga tagtcagccg tgttgtttct gtattcggct gcgtgggccg ggtcggcttc
     9001 caccatggcc ttggaaatat gctgcaccat cgtggcggcc gcggacggat ccagccacac
     9061 gtgagggtta ccgcccgcat ggtggtgcgt gtgggcatcg gcagtcgatt cgggatgatc
     9121 cgggatcaat tcaatgtctt tagaggtcgt gaccacctgc aaggaggcgt ttcccgcatt
     9181 cttgaccagt gaggataccc acacttcgag tcccgctccg acctcaaaca agagggcagc
     9241 tttgcggacc gcaaccagat cgctgggctt gggcgagtag gtatgctcgc tttcatatcc
     9301 ggtcatgagc gagacgacat ggacatgaga cccgccgatt tgttgcaccc aatctttcaa
     9361 gacggggagc gtcaccacga cgttgagcgg ttcagctgcc gatgccgtgg gtacagctgc
     9421 ggaaaggttt cccagcatgg tcaggaaaat gaaaagagtg agcaaaatcg tgcgaaacat
     9481 ccggcggacc gtaacacccg atgtacccaa agtcaacgcg atgacgcatg caaaccgagt
     9541 gctctgcagc agcgtaccgc ttgacccgtc tcgtggtttc gattagcgta gcgcccgaat
     9601 gccaggtcga tctcaggcgg ttgtgttgaa tggggggcgg gatgaaggtt gtcggactca
     9661 tgtccgggac atcggcagat ggaatagacg cggcgctggt gaatatcgtt cggcgcggag
     9721 gccgaccggt catcacgccc atcgccttct ccaccgtgcc ctatcctcgc gccttgcagc
     9781 agcgagtggt ggatctgtcg cttcatggcc aagtggcgga gatctgccat atgaatgcgt
     9841 acctcggcga attgtttgct gaggccgcgc tcaaggtgat tcgggcggcg aaacatcagc
     9901 cagccgatat ccatgccatt ggatcacatg gccagacgat ccaccacttg ccaaaaggac
     9961 ggcgtgaatc cggcgtcggc ctgatccgat cgacattaca aatcggtgag ccggcagtga
    10021 ttgccgagcg cacgggtatc acgaccgtag ccaatttccg gccgcgagat atggcggctg
    10081 ggggtgaagg cgctccactg gttccctatg cccatgccgt tgccttcagc catgcacgcc
    10141 ggacgcgatt ggtggtcaat atcggcggga ttagcaatgt aacctatctt cctgcaggcg
    10201 gcgtgatggc tgatgtgcgc gcgttcgaca ccgggcctgg caacatggtg ctcgatgcca
    10261 ttatgcagga aacgacaaag ggcaaccgta cgtacgatgc cggggggcga ttggcgcgaa
    10321 agggcacgat acaccaaccg ctgctcaccg aactgatggc ccatccgttc ctgacgcggc
    10381 gtccgccgaa gtccacaggt cgtgaagagt ttggtgcgcc cctggttcgt cgcttgctcg
    10441 cgaagcagaa gaaagcgcgg ctgtcgattg aggacctgtt ggcgacctgt gcggcctgga
    10501 cggcggaagc catcggttca tcgcggcgct gggtccccgg caagatcgat gacgtcatcg
    10561 tcgggggagg cggggtctcc aatcgagcta tcatggcgtc gctccggacg gtgtttgccc
    10621 ccgcaccggt gctgacattt gatgagtgcg gttggagtag taaggcgttt gaggcgaccg
    10681 cgtttgcgtt gctcgcacat gacgtattgc acggccactg cacgaatgtt ccgcaggtga
    10741 caggggctcg tcatcccgtc ttacttggat ccgtggttcc cggcaaagcg gacatacgga
    10801 ggaggaaccg gtatctgccg ggttaaggga ctcgcatgaa catggacgta ctctatcctc
    10861 tcctcgcgat cggcgcgctg gtccttttgg gaagtctcgc gtgggaaaca ttcgggcggc
    10921 gacgtgcgcc ggctgcgtca tccggcagcc tgccgtccgg actgaccttt gcccccaagc
    10981 ctctgctgac agaccaggaa gttcagctct acaacctgtt acgccttgcg gtcgaagatc
    11041 gatatctctt atttactcaa attcctctct ggagcgtatt ggatttgcgc gttccctccg
    11101 ggatgcctcc agccgaaacg ctgcgggagc tggcgctcac acgcgccacg tttgtgctgg
    11161 tgcatcccgg cagtcggttg gtggagaaag tggtgcaggt cgagccctcg gcctcgcagg
    11221 cagaagggca ggggacggca aactctctat tcgatggtgc gctgcgcgcc gccggcgtgc
    11281 aactcgtccg actcagtcca ttccatacgt atacggttcc cggcctcgtg acggtgttgg
    11341 atctcgccga tccggattga atccggccga gtcagtgagg aagatcggtt ggtcgacccg
    11401 tagggaccga atgcggcaag ccggtcaatt cggtccagct gcgattggat ctcccttcgc
    11461 agcagcggct tctatggcct tacgcgcgac ttctgcctcc gggctatcag gatattcgcc
    11521 ggtgactcgc tcgtacatca tttgagcctt ttcaaggtca ccctgtttcg tgtagatctg
    11581 tccgatttta agggtcgagg cggcgagttt ctggctcatg ggatagtctg aaatcagttt
    11641 gaagaatgat cccagtgcct cgtcgtgctt tcccatacgg tactggcatt cccccatcca
    11701 atattgcgca ttggccgaca atgatgagtg cccgtggagc tcaaggaaga agcgaaaccc
    11761 ggcgagcgct gcagggtagt ctttgtggcg gaactcgtcc atcacgcgat cgtagaggcg
    11821 gcgtgaggca tccggttgct ggggtgacgc ggcgaacaga tcggatgcgc tcgcgaggat
    11881 gctgaacaac gccaatgctg cagcgatacc tcgcaccttt tcacggctga gttgaaccat
    11941 ggtcatagaa gcccctcttt cttctgtggt cggcgcgtct ggaacccatc cattattgtg
    12001 gccgtggata tgaaagaacc gcaccagccg gaggcaatcg acgaacttgt tgtgaaattg
    12061 tatggttggt taatccgagc ggctcgggga tcagaaagct ctgtgcggaa agcacacagg
    12121 attgtacgga aaagagccga agggtatgtt gagggcaggc agttgacaga aaacacgttg
    12181 tcgtttgttg gtggttagcg ctccattgtg gagtgaatga cttggtcgag ttcctagagg
    12241 attgaaacgg tcgtatggat acatctcacg aggctggttc atcgggcgca ggcaaggacc
    12301 ctctcatcga accgccaccc aatcccaaag gcgggaagac attgattgtc gatgcccgcg
    12361 atcccgatgc ctacctttgt cccagcgcgg cgctcaaaga cgcgggcgag gatgaccagg
    12421 tatttgtccg ccccggtgtg tatgaagata aggtgtttgt tgcggataag ccggtgcttc
    12481 tcgtcggggc tggtcgcgac caggtgcaga tatacagtcg tcgcggaggg ccactgtatt
    12541 tgcaacgagt gcccaacggc atgatttccg ggattacctt ccggtatgtg ggcagcgatc
    12601 aacattccgc catgaacgtt ttagactcgg tctgcaccat tacgcggtgc cgggccatgg
    12661 aaggcatctt atcgggggtg gtgatctacg gtcccgaggc gaggccgacg tttatcgaca
    12721 atgaggtctg ttccaatcgg gaatccggca tctttgtctt tgcgggggcg cagcctcggg
    12781 tggcgcagaa tctgtgttat ggcaatcacc attttggttt ggcggtgcga gatccaggca
    12841 gtcatccgga gtgtgttcgg aatgtgtgtc atagcaacat gctgagcggg atgcttttgt
    12901 tccatcatgc ggaggcgctg ctcttggata atatctgccg cgacaatcag cattggggct
    12961 tggtgatgac gccggactcg catccgactc catcgcagga ggaattggtg gcggggaacg
    13021 tgttcctgcc caatccccgg ggctcggtgg tggtgacgga gcagcctctc gccgatatcg
    13081 ggcggtagcg gtgagcgatg aagagtaggt ggattgaact gacctagcgc gcggcggcac
    13141 gcgggccctt tacgggaggg gagcggggac atcgggagaa tggccggccg gtgagtgaaa
    13201 atgatagcgg cctcccccct gctgtttggc tgcgtacatc gccttgtccg cgtgcctcag
    13261 gagctcgtcg agatcctcgt cgtccgaagg atagagcgtg atgccgatac tcacgccgat
    13321 cgagatatta tgcggcccga tctgaaatgg cgagccaatc gagtctacga ttcgcttcgc
    13381 gacaaccagc acatcgtctt cgcctgagat cccttcgaga atggcagtga attcatcgcc
    13441 gcccagtcgg gcgaccgtat cgacctctcg cacacattcc atcaagcgtg tggccacttc
    13501 tttcagtagc tgatcgccga tatcgtgtcc gagcgtgtcg ttgacggctt tgaagcgatc
    13561 cagatccagc agcatgaccg ccagcggctg atcttttcgc ttgctgcgtg ccatggcgtg
    13621 aatcaaccgg tcgcgaaaca gggcgcgatt gatcaacccc gtaagcggat catgctgggc
    13681 aaagtaggtg agccgctcct gcgccttctt gcgttcaatg gcatagagaa ttgaccgtgc
    13741 caaaaactca gttccacctt cccccttgac cagataatcc tgcgcgccat gttggagcgc
    13801 ctgcagcgcg agcggatggt catcatgccc gctcaagacg acgaccggca catcggggct
    13861 ggtcgccaag acctgtgtga cggtcgccag gccatgggtg tctggaagcg agagatcgag
    13921 gaggacaaca tcgaaccgct ctcggctgag tcgggtgagt ccatccgcca ggacggaggc
    13981 atgggtaatc tcgaaacggt ccagtgacca ttcggctagg aggtcttgcg tcagttgcgc
    14041 atcgacggaa ttgtcttcca cgagaaggag tttgatcatg aggccgcgtt cacactccga
    14101 tcacagttgt accggcaggt tgtgcacatc atgatgaatc gcaaggttgg gcgaggattc
    14161 agtgcggggt ctctcccggc ccacggtttc ctggagataa cggaccggct tgatcgggat
    14221 tctagcatgt cgcatcgaac cgtcaaacaa cagcctgctt ccccgcggca tgtgttgtcg
    14281 agaccatggt ctgcgccgta gcgatgtgcc tggttagtcc ggcgtgctcg caacgttgta
    14341 gattgcctgc aacggacacc aacgagctga ttgccaaatt acggccgcgg gatggcccca
    14401 aattggctca tctcaacatc ctgccctgcg cgatcgcaat cactgttcag aacccgttct
    14461 ctaacctcct gatgttatag gccgtgacca gttttgtacg ggccggcgaa gtggcatgaa
    14521 ttctgcgcaa gaagatctgg gtcgcaacgc aatgccctgc acacacagtg cagaggaagg
    14581 ggagggccct atggcgcagg aacagtctgc tcgagaccag gagagttttt cccgcacatc
    14641 ttctgttgat gactgcctgg atggaaagac gcgcacatca gtctcctggg cgcttccatt
    14701 ggcgattgcc ggggtgttgg ttgtggggga ttccgcctct gcggaaaacg acatctacgg
    14761 ctatgtggga gcaaacggcg tgttcgagat gacgaatgtt ccgacggatg gacgctttcg
    14821 tccggctgag ttgcagcgta gccgattgtc acatcgagca tcagtcgaag aagtcgagga
    14881 tgccgttgaa cgctatgcct ggcagttcca cctgcaccca gccctgctgc tggctgtaat
    14941 caaggcagag tcggatttta atcccaccgt catttcacga gctggagcgg tgggactcat
    15001 gcagttgatt cccgaaaccg ccattcgtca cggtgtgcac aacttatatg atacgggcga
    15061 taacattcgc ggcggcgcgc ggcatttgcg gtatttactg gatcgattta acggcaacat
    15121 ccggctggct gtggcggcgt acaatgccgg cgaacggcga gtagaacgat atcacgccat
    15181 accgccctat cccgaaactc gcgactacgt ccggaaagtg atgatctact acaagagctt
    15241 ccgtggagat tatcaagcca ctccgggaaa agcggtgctc ctggccatgc atcataaacg
    15301 attgcagctg gagcccctgt cggtccccgt ggattttcgc catggtcgcc ctgcagcgaa
    15361 aaagtaacga gcaataaaag gctgcaggct tccaggggcc tacctgcgac aagactatcg
    15421 gaggcgaaag ggcggagccc cgaggtcggc tgttcgcggg tctgtgatga acctgcactg
    15481 ggaggctagc gccgaccaac gcgcagattg ctaaaaactg ctgagcatcc ctctcgaaat
    15541 aaaagcattt aaaggaacat cccgtactcg ttcatcgatt ggcgtgatcg tagtttcaca
    15601 tctgaactta tatggcttcc acaagagggt gaagccgtat atactcattt ccactgtcag
    15661 aattcaggaa gatatccctg tcaatattgg ggcatgcccg ggtttcgcct aggtaaaaca
    15721 acaccgttcc tagggcacta tgggcaagcg ggcggccgtt gacaatagcg cgtcagcgcc
    15781 cggctgctaa gctgatgcgc aattcccgca cacattctgg ggacgcgaaa ccctatcgcc
    15841 atcgtacagc ttctacgcct tgccggattg tgtcgcaaaa ttagtggtcg cagtcgagaa
    15901 cagggcccag atgtaatcct gtaaatggga tgcatcagct agcccgtttt tgaggagcca
    15961 tatcttgcct gacacccctt tctccgaatc acgcagcgga ccgcccagcc atggacattt
    16021 gtcgcttcga agtctgttga tctctgcact ggcatggcca tttaaaacat tcatcaggcg
    16081 gctcatcgga gaagtgttct atcagcaggc ccgagtcacg ttgcttcacg aacacggtgg
    16141 tgtagggctt gactggaggt ggggaaattc tctccggcgc gtccatccta ttcggcgtga
    16201 tttcggctgg cagtcaggac gccccatcga tcggtactat accgaagacc attttctccc
    16261 tgcttaccag gcggatattc gcggccgggt gctggaaatc ggcgatgatc gctacacccg
    16321 acaattcggg ggcgagagag tgaccctctc ggagatcctc aacgtgaatc cgggtatggg
    16381 gggcacgacc atccaggctg acctcactga tgccaaacag attccctcaa acacttacga
    16441 ttgcgccatt ctgactttca cgttgcagtt tatttttgat gttccggcgg cgctcagaac
    16501 actcggccgt attttgcgcc ccggaggagt gttattggtg atcgttcctg ggatcagtca
    16561 gatcgcgcgt tatgacatgg acaattgggg cgagttttgg cggtttacgt ccctgtcggc
    16621 ccgtcgactc tttgaacaag tgtttcgtcc ggaagacatc acgatcacga cctacggcaa
    16681 cgtgctatcc tcccttgcga gtttgcatgg actccactcc acagagctca ccaccgccga
    16741 gttagaccat tgggaccgcg actatgaact cgtcattggc atacgtgcgg taaagcgtcc
    16801 agctgccaac acgagcctct agagctgcct cgatctcaag aaatgtctca atgcttacag
    16861 ggagggaggc tgatggctcc agggttcgct gacgccttct tcatcaagcg aatcacatgc
    16921 ctcctagaag aaactcgaat cagatcagta tgtggtgtct ttagtggaga taaccgggag
    16981 ggagggcatt caaaattaac gcatccggca ccatgcacag cgctcggatg gtcttatcaa
    17041 gggcagggtc atggtatgtt gcggccgtga acgattcacg atctacgtaa tgaggattga
    17101 gccgagagag tgtctcattg atgtagagtg acggatgagc caataggcct tgccgtaata
    17161 cttcattcgg gagcgtatcc gcatgatatt ggcagatacc ggagagggcc ggatgtgatt
    17221 gaagaaatgt ttccagccgc cattcataat ccagaagctt tgaaaaatct ctttctggac
    17281 caaactccaa ggtcatgtca cccgtacacc agaaaccggc atgcccgtcc tgcaaggctt
    17341 ggttcaatcc ttcctcaagc attccgagca ttcgatcaat actgaaatgc ccatcggtga
    17401 gatgggtcgg gtttgaagat aagacgagac ggcctctttc aacttcttgt ggaatatcga
    17461 ttcccgcagc cagcaaatag gggcgcatgg cagcgatcat ggccgaggta ttgaaataca
    17521 ggcagcgata attctcggta agtttttgcc gaatgacagc cgagagccca gaaaaatgcg
    17581 tcgctggtga gcccttataa atcaggcatt gatggcgagg agtcgatgcc gtgatctcgg
    17641 tgggtgtggt caacgcgcta tgcataccct agatactatc gcataaggga tatttgatca
    17701 gctgaaattg aataatcagc ggcggccgct ttcagtgtcc gccccctcca cggcgcgctc
    17761 ccatcaataa tgtgttttga atgtaggcta gttccggtgg ctgagtcttg acgatcaatc
    17821 atgctacttg gccgctaact gagcggcgac cttacagttc ctgctggatg ctcagctgga
    17881 ttttgccaca ccgttagcac aatcggctgg ggtgaacccc tggggtgaac cccttcactg
    17941 agacagtgcc tgaggaacag cgacacccca gctcgtgtgg cagggtgagg gacgatcttt
    18001 cgacaagtgg tgagatttga gtccggtttg agtagaatgg aagcagctag ccgactcatt
    18061 cagatgggcg agtgcggagg agggttcgac gatgcgacaa acaatcaaag cccgctacca
    18121 tgacggagtg ctacagccgt tggaacctct ttccctggcc gatgacgcgg aagtgcaggt
    18181 cacagtcgat accgaccttg cgctcggtac ggacgaaatt ctgcgtcgtg cagcgcaggt
    18241 ctatcaaggg ctgagtgccg acgagatcac gcaggtcgag tccatcgcat tggatcggca
    18301 gcacttcttc cgtgagccgg ctgcctaatg ccgcaaccgg ctgtcctcct cgatactgat
    18361 attctctccg aactgctcaa gcaacatccc ctcgtcgtgc agcgtgtgcg gagctatctg
    18421 acggagcacc agcggctggc cttctcaatc attacgcgct atgagctcct gcggggactg
    18481 aaggcgaaac aggcacggac tcaagaggca gccttcacgt tgctctgcca ggccagtctc
    18541 attctcccga tcaccgacca agtcgtcgag cgtgcggcga cgttatacgg cgatctccat
    18601 cgacaggggg ctttgctgcc cgatgcagac ctgctcattg ccgctactgc cttggacgcg
    18661 caacgtccgt tgattacgaa caacctcgcg cacttccagc gcattcctgg cctcatcgtc
    18721 gaaagctgga aacggtcagt ctaacgaagc gatcctgctt ccttacgatc tgcccatcga
    18781 tcaaatccgt acccttgaag tcggcaaagc tgtgtaaccg gccccatgat gggatttact
    18841 atatagattc caattccgct gcctggaggg gaaaaccggt cgatcgagag gagattgccc
    18901 accgga
//
LOCUS       DAOQEW010000063        18688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_762_length_18688_cov_7.781463, whole genome shotgun sequence.
ACCESSION   DAOQEW010000063 DAOQEW010000000
VERSION     DAOQEW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_762_length_18688_cov_7.781463"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLS35_08765"
     CDS             <1..1158
                     /locus_tag="PLS35_08765"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNI18793.1"
                     /translation="RIARSFGAEGIGLCRTEHMFFAEDRIQIMQKMILARKREEREMY
                     LDQLLPLQKQDFIGLYREMKGFPVTIRLLDPPLHEFLPKREDLMVEIAQLELTSGSQT
                     VLEEKKRLLTRVEELHEFNPMLGLRGCRLGITMPEITKMQARAIIEAACELAKEGTKI
                     VPEIMIPLVGMVSEMKAQKDLVREVAAETMKRYGVKLSYLVGTMIELPRAAVTADGIA
                     EEAEFFSFGTNDLTQTTFGFSRDDAAKFIDFYKTANILESDPFAVLDREGVGSLMRTA
                     ITGGRKTRPTIKLGICGEHGGDPSSVEFCHQLGLDYVSCSPYRVGIARLAAAQAALAE
                     AEKEKPKPAKSKPKAAKPSAVKSAKKSAKSAPAKKRPVAKRAVRRSKRTSR"
     gene            1155..2282
                     /gene="ribD"
                     /locus_tag="PLS35_08770"
     CDS             1155..2282
                     /gene="ribD"
                     /locus_tag="PLS35_08770"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNI18794.1"
                     /translation="MKLSSRDREVMALALRLAAKGRGHTGPNPMVGAVVVNRGEIVGQ
                     GSHRKAGGPHAEVLALSQAGSRAKGGTLYVTLEPCSHLKKRTPPCVPLVISSGVRRVV
                     VAMVDPNPQVQGRGIAQLKRAGIQVEVGCLEPEARQLNETYIHWVQTGRPFTILKAGM
                     TLDGQIATATGESQWITDEAARMQAHQLRADVDAVLVGIGTVLRDNPQLTARTGGDPV
                     RLAARQPLRVVVDSRLRIPSRAAVLKRQEEAHTVIATTRAASVTKVERLRGQGIDVLV
                     LPAAGGHVNLTALWARLGQLGVTSVLVEGGSDLNAAVLRAGLPQRLMCFVAPLLLGGQ
                     DAKGVLGGLSPRRLREAVLLNNLRMTPVGRDMLIQADFPTR"
     gene            2290..3618
                     /locus_tag="PLS35_08775"
     CDS             2290..3618
                     /locus_tag="PLS35_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18795.1"
                     /translation="MRSSLRPAYRVHPRRPGLLLSLVCIWLALLSVSLTHAAEESSST
                     DLAAQVWQYLTTQDSEQANSALASIMQRPDATLQAVQDILRKGQPHGLQPVGVLADEQ
                     VMVRDRPYRVSLSIPQSYEPTKDYALVVCLHGAGFTGEAYLDRWKSRLGEQYILACPT
                     YPAGAWFTRRAEDLVLATVQLVRERYRIDPNRMFLSGMSNGGIGAWLIGMHHAPLFAG
                     LAPMASGIDDVLFPFLENLRTTPVYIIHGSQDQVMPVELSRKLAGELKNLDYPYIYRE
                     HDRTHAMAGGHFFPREELPDLVKWFDTQHRTPVPKTVTVVRDASHLLPFGWVRIDATD
                     QIASFSEDLVDKRDDSIRKRIYARLTAQVTGPNRVDVRTDRVRQYTLYLNEDLVDLSK
                     PVVITTDGQPSFEGLLEPHVETLLRQARLRQDPAQLFPAHVTVTVPQRAS"
     gene            3615..4424
                     /locus_tag="PLS35_08780"
     CDS             3615..4424
                     /locus_tag="PLS35_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18796.1"
                     /translation="MTGGGEMVRRIGPLAAVLTAALALCDSTGQAEPERCSLPSHHVG
                     MVFPLDQVDAAWTCRLEPIIAHYTTANKVGPIRTLLPEDVYLYLLEHPVMAAALVNRL
                     DLGLYKAEWRGPDEFWATDGEGTEGIVHPLYQDRSTRIYYVEGTHDGRFLPRVSGKAL
                     VLFKLQPVKDGQGSESMDNTMVAYLRLDNRLYSGLLSMLRPLIGNVVTRQVVKAFDAA
                     RRLAGVMRDHPDQVLFEATDPPGLPDEQVAFLKAALASLHNPGQKPSPALP"
     gene            4432..5607
                     /locus_tag="PLS35_08785"
     CDS             4432..5607
                     /locus_tag="PLS35_08785"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI18797.1"
                     /translation="MTTSRSFFYLCTLGVFCFISYNLVRMPVLSLFAESLGAGPERIG
                     LIVSVSTITGVLLKLPSGALSDIYGRKMLLRIGVVAFGLPPFIYPFISDLNALTALRF
                     VHGLATAIFAPSALATVADLYKERRGAAMGTYTACTQSGSLLGPFIGGWLAHAAGFPA
                     AFVTAGVFGCIAILIFFSLHLDEPPPRVREKGLAPVITEMGKGFLAVARNRKVLITSS
                     TDAAKMIANGALMAFLPLYGLSVGLNAGEVGLMFSVQAVTSFLSKPVMGRISDRVGRQ
                     PLIVLGLVICAATFISIPHVGSFAVLLMLSSGFGFGEAVVSSSSAALVADSSEFKRLG
                     AGMGMQGTVMDIGHASGPLLAGFLIAHASYQGAFAVIAGIQILAAVAFWATMRTILR"
     gene            5787..6443
                     /locus_tag="PLS35_08790"
     CDS             5787..6443
                     /locus_tag="PLS35_08790"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNI18798.1"
                     /translation="MFSGIVEEMGAVSILNKGLAGTRLTIMASTIMTDLTIGASVSVN
                     GACLTAVARTDHDFSVDVSPETLSVTTLSDLTTGSPVNLERAMKLNERIGGHMVSGHV
                     DGIGAIRSRHQDGNALVLEIEAPKDILRLCVHKGSITVEGISLTINEVTERSFLVAII
                     PHTAKVTTLGLKQVGDKVNLESDLIGKYVERLLQDRGLLPPKPTPVIDKDYLQRRGLI
                     "
     gene            complement(6660..7136)
                     /locus_tag="PLS35_08795"
     CDS             complement(6660..7136)
                     /locus_tag="PLS35_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNI18799.1"
                     /translation="MVTGIILLGGVVPILAADDTPKAAGQAPAQAKKAPEKKSVKKGE
                     GRKTTTAPVKSPATKYASAETAAKAQACFGEAPKIESVKPDEGKAGEKVTISGMNFGP
                     AECLRSLSFGPGNAATFKLDSPTQITATVPASKRKGMAIMTVTTASGEDSKGFLLK"
     gene            complement(7228..9117)
                     /gene="acs"
                     /locus_tag="PLS35_08800"
     CDS             complement(7228..9117)
                     /gene="acs"
                     /locus_tag="PLS35_08800"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNI18800.1"
                     /translation="MSEKIDTLLKEGRVIQPSARIKAAAHIPDYDQAYKASIADPEAF
                     WGGVAKELEWFSPWSRVLEWNYPWAKWFVGATCNIAYNCLDRHTNGWRRNKVAIIWVG
                     ENGEERIFTYGELLRQVNRCANALKALGLNKGDRVTIYLPKIPEQVVAMLACARLGII
                     HSVVYSGFSAPALASRIQDAEARLVITADVGYDRGKTIPLKPVVDAAVQSCPSVEKVV
                     VVRRDALSVALTAPKEIDWADWLKEQNAVCAAEPLDAETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYITTKYVFDLKEEDVYFCVADPGWVTGHSYIVYGPLLNGATILMAEGKP
                     DYPNPGRWWDLISRYGVSIFYTTPTAVRLLMKYGEEWPKKYDLSTLRILGSVGEPINP
                     EAWEWFYRVTGSDKPIMDTWWQTETGAILVTPLPSVPLKPGSATRPFLGIEADVVDKE
                     GNSLPANAGGFAVIKKPWPAMMRTIYKDPDRYKVYWNTIPNCYTAGDVCRKDQDGYMW
                     FMGRADDVIKVAGNRIGTAEVESALVSHEAVAEAAVIGKPHRTAGEAIKAFVILKQGY
                     QDSKELVQSLKDHVLKELGKIAVPQEIDIVSSLPKTRSGKIMRRVLKAKELGQDVGDI
                     STIED"
     gene            9346..10725
                     /locus_tag="PLS35_08805"
     CDS             9346..10725
                     /locus_tag="PLS35_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI18801.1"
                     /translation="MRSPRSLTFPRMITCLAALFVAGMSTDHAAAQVGKPEGLYYKSW
                     AVVVGIENYLVAPKVPGALESSHAVAEALRGLGFDEVIEIQDKEAASKRLLHILNDYL
                     PRKVGRQDRIVFFFTGHAGLTQDSQAKDSGFVVPWDAQLNNPAKAITLDQLKEFSRRS
                     ASKHILMVFDANLRGWDVTAPQPLSLEGRLSPEDDTEKRSVQVLTAAEKEESVVRTSG
                     QSAFVTALVAGLKGAADLNKNGWIMASELAAQVKQDVERETKGAQHPQLLQLEGDGDV
                     ILVEGRKAMFSVGKEPSTEADRMKEARVQYEQAFALLQQQKSAEEALERLNRAITYDP
                     SFGDAYVLKSFIRLEVLPNLEESLAAAQAAVQHAPQNPDSHYSLALALEKKGQHQEAE
                     RAMLQALAVNPAYTDVYFSLGTLYADHLNEPQKSVDAFRRYLELGGQSERATRAVQGD
                     RAPGEKPAP"
     gene            complement(10817..10987)
                     /locus_tag="PLS35_08810"
     CDS             complement(10817..10987)
                     /locus_tag="PLS35_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18802.1"
                     /translation="MADVQIEDGIIRVVQLDIQDPKAAAVLAEYPQVRWPEITRRALK
                     IGLGYLKGGGKD"
     gene            11224..11790
                     /locus_tag="PLS35_08815"
     CDS             11224..11790
                     /locus_tag="PLS35_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18803.1"
                     /translation="MSRVEGAFSKFTGSALMLVLLIGLTACGGPPNWVKKGSGAFNEK
                     SDKSFYGVGSVVGVRNEPLAWDTAENRSRAEIAKTFETYTAYLMRDYAASTTAGDFSR
                     NSEEQNIERAVKTFSAVTLNGVKPMDRYKDEKSGTYYVLTKLSLEDMKNNLEQAKELN
                     SQVRDFVRKNADRLFDRLEKEEDKRATK"
     gene            11830..12459
                     /locus_tag="PLS35_08820"
     CDS             11830..12459
                     /locus_tag="PLS35_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNI18804.1"
                     /translation="MIEWREHSVLRTCAVGLLVGCAAVVSGCGHETKVTRVDTGTVTD
                     LSGRWNDTDSQMVAEAMVKEALANPWLGNFTKGKNRQPVVIVGTVLNKSHEHINVQTF
                     VNDLERELTNSQKVTFVAAKGERDEVREERREQAVHAREDTQKAPGKEIGADYMMRGS
                     IATILDEQDGAKAVFYQIDLEMVDLENNVKSWFGQKKIKKVIEKKRTIF"
     gene            12537..13970
                     /locus_tag="PLS35_08825"
     CDS             12537..13970
                     /locus_tag="PLS35_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18805.1"
                     /translation="MHSVIGWCLLWVLSGCGLSSNHYLLIDQSLAAHDARRADAIMAE
                     AESEYGPRNRLLYSMDRGMTLHLAGEYMQSNAQLESAAQEVERLYTRTIRTETAAFLT
                     NDNVLPYEGDPYEHVMINIIKALNYAAMGQLMEAVVEARQIDHRLNVLSDAAKEKDGY
                     REDAFARYLTGVLYEATGDLNNAFIAYRKAYDIYDATKSWARTPMPPMLRADLLRTTD
                     ALHMTTEFEEYRRLFPETRWLPRADQQNLAQVVVIGYNGRAPRKEDAFLDIPISLSAL
                     QLVLLNRGVIHASNRQERRVADSVLYGLNGRVVRVALPKLILQKTQVVHETVTLIPRG
                     GEPISATSELVYNGTALAERSLSDRLPGITTKALARAAVKFAAAEAATRGSQHAVNKG
                     DAPWVGLLVGVLAHGLAVASESADTRSWRTLPDEIQISRLWVPPGEYESRIQAMVRGN
                     GTPQVEGPRPLVLRAGQTLFLIERVML"
     gene            13985..15103
                     /locus_tag="PLS35_08830"
     CDS             13985..15103
                     /locus_tag="PLS35_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNI18806.1"
                     /translation="MVSDRGTRISLVALVMLLCAGCGWVGGPSRPSWIDGSSTQFPST
                     QYLVGVGQADSRPQATEQAYAAVSRIFKAEITAQAKDWESYLVVESRGQTSTQRKLIL
                     DNVTRVTTDKVLENVQVLDTWFDQKARQYYALAGMNRAQAESAMLERLADLDRSIQTE
                     VTEAHQAQDKLSHVRNLKRAAKNLVLREAYNTDLRTIRSNGQGHPAAYRVAELTAELE
                     HYLAANMGMAVEVSGEQAEPLERALIEGLTQEGFSVVGSGPGAATAELLITGTVRMWP
                     IQVPDPQFRYVRWCTDAVIEEVSTHRVIGAVSKGGKEGHVTEREAAAKAVRVMQQEFS
                     ADLAQAVAAHIYGERDLSVSSSAPSGCPKEAGQPPLSR"
     gene            15138..15386
                     /locus_tag="PLS35_08835"
     CDS             15138..15386
                     /locus_tag="PLS35_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18807.1"
                     /translation="MAKTGTRKAGEQAGGGRRMSSQAMKKHLRERLTADTQQVLKSDI
                     VSIFRKQGYYEELPLDELQDRLSKLQSPLADSLLKGRV"
     gene            15388..>15812
                     /locus_tag="PLS35_08840"
     CDS             15388..>15812
                     /locus_tag="PLS35_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNI18808.1"
                     /translation="MPYYYFDSTALVKRYSMERGTRIVNKLMVKRGKVAILPMWSVTD
                     FYSALSVRSQQGEITRDDCYSVLYKFEMEASQGLFQFISPTMDTYLAAKELILDYPAL
                     RSPQLLHLALALELKPLRLTVVSADKQLLAACRPAGLHI"
     assembly_gap    15813..15912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15956..16498)
                     /locus_tag="PLS35_08845"
     CDS             complement(15956..16498)
                     /locus_tag="PLS35_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18809.1"
                     /translation="MRNGIHIGRCEHCLAASILSFFILVAAAGIGQAQVMGEEAELDR
                     LRAKAEDAMGNDDAEGAAMNMGRAALMAAQLGKRQTEPALRQVFKSTEHLYRSQEHGY
                     RGLALFRRAGGELPASAGVCGSLQLAQLELQHAQETLASSETPDRPGAVSPKLPAIRQ
                     TADDWAIVLASMMGEFRCPN"
     gene            complement(16495..17571)
                     /locus_tag="PLS35_08850"
     CDS             complement(16495..17571)
                     /locus_tag="PLS35_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18810.1"
                     /translation="MRCILSLVILGVLLLFGDPSWARREALTAEQKSQMERIDRVLIT
                     VLALSDKGSVDAGPLAEVAANRMKEFGYTPVTDPTQPHDVELKIKCEQKKTWEGTTTM
                     GSDADLPDAPSRIWKGPACQLGYLLNGKKMAWRKEVRTDFDDAQAAATLAKAGDPTSF
                     AMGRLQARLEEYDFPALITAEWGQEARLFRLYDDPKTPVARKVKLVALFGDLFSVKAI
                     PRLLDGLNGPDRSIAKASALALGNIGQKDTIPVLIKTMKNGAPDLRAPAAKALGIVGA
                     LHGDFTIVDPLLETLNTEDVAVKTEVAWALGKLPDMRAYEPLFALQKSLYHVHENDAD
                     PNLVKLKEAVNWSIKQIDTWEHVQ"
     gene            17688..18518
                     /gene="proC"
                     /locus_tag="PLS35_08855"
     CDS             17688..18518
                     /gene="proC"
                     /locus_tag="PLS35_08855"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNI18811.1"
                     /translation="MLTGRQIGFVGGGNMAESLLAGLLRKGLSSPGRLVVSDPLASRR
                     ELLSRNLGVSVTPDNQAAVRGADIVVLCVEPQVLDDVLRELAPSLPSDPVIISVAAGY
                     PLSRVQHHLPSATRLIRAMPNTPSAIGEGVTAMSLAPGVSSEDRETARQLFESVGMAV
                     VVEERLLDAVTALSGSGPAYVFAMIEALADGGVLGGLPRSTAQLLAAHTVAGAASMVL
                     EQGEHPAVLKDRVASPGGTTIAGLYQLEQRKLRATLMSAVEAATQRSQELGKTENHHL
                     "
BASE COUNT         3667 a         5307 c         5685 g         3929 t
ORIGIN      
        1 cgtatcgcgc ggagtttcgg cgccgaagga atcggtctct gccgcaccga acatatgttc
       61 ttcgccgagg accgcatcca gatcatgcaa aaaatgattc tcgcccggaa gcgcgaagag
      121 cgggaaatgt atctggatca gctgctgccg ttgcagaagc aggactttat cggtctgtat
      181 cgtgagatga aggggttccc cgtcacgatt cgattgctcg atccgccgct gcacgagttt
      241 ctgccgaagc gtgaggacct catggtcgaa atcgctcagc tcgaactcac cagtgggtcg
      301 cagacggtgc tggaggagaa gaagcggttg ttgactcgcg tcgaagaact gcatgaattc
      361 aatccgatgc tcggcctgcg tggctgccgt ctcggtatta cgatgccgga aattaccaag
      421 atgcaggcgc gcgccatcat cgaagcggcg tgtgaactgg cgaaagaagg caccaagatc
      481 gtcccggaga tcatgatccc gctggttggc atggtctcgg aaatgaaggc ccagaaggac
      541 cttgtccggg aagttgcggc cgagacgatg aagcggtatg gcgtcaagtt gtcgtacttg
      601 gtcggcacta tgatcgagtt gccccgcgcg gccgtgacgg ccgatggtat cgcggaagaa
      661 gccgagtttt tctcgttcgg cacgaacgat ctgactcaga ccactttcgg cttctcgcgc
      721 gatgatgcgg caaagttcat cgatttctac aaaaccgcca atattctgga gagcgaccca
      781 tttgcggtgt tggaccgtga aggtgtcggt tccctcatgc gcacggcgat caccggtgga
      841 cgtaaaaccc gtccaaccat caagttgggc atctgcggcg aacatggggg cgatcccagt
      901 tccgtggagt tctgtcacca attgggattg gactatgtga gctgttctcc gtatcgagtg
      961 ggaattgcgc gattggctgc ggcgcaagca gcgttggcgg aagccgagaa agagaagccc
     1021 aagccggcga agagcaagcc gaaggccgcg aagccgagcg ccgttaagtc tgccaagaag
     1081 tccgcgaaat ctgctccggc gaaaaaacgg ccggttgcga aacgcgcggt gcgccgctcc
     1141 aagcgtacga gccggtgaaa ttatcctccc gcgatcgtga agtgatggcc ctggcccttc
     1201 gcctcgctgc gaagggccgg ggtcataccg gtcccaatcc catggtcggc gcggtggtcg
     1261 tgaaccgcgg agagattgtc ggacagggct ctcaccgcaa ggccggtggt ccgcatgccg
     1321 aagtgctcgc cttaagtcag gccggttcac gcgccaaagg cggcactctc tacgtcaccc
     1381 tcgaaccctg cagtcatctc aaaaaacgga cgcccccttg tgtaccgctg gtcatttcct
     1441 ccggcgttcg gcgcgtcgtc gttgccatgg tggacccgaa tccacaggtg caggggagag
     1501 gaatcgccca gctcaagcgg gcaggcattc aggtcgaagt cggatgcctc gaacctgagg
     1561 cgcgacagct caacgaaacg tatatccatt gggtgcagac cggccggcca ttcaccattc
     1621 tgaaagccgg catgacgttg gatggtcaga tcgccaccgc caccggcgaa tctcaatgga
     1681 ttacggatga agcggcccgg atgcaggccc atcagctgcg cgccgacgtc gatgccgtgc
     1741 ttgtcgggat cggtacggtt ctgcgcgaca atccacagtt gaccgccagg acgggtggtg
     1801 atcccgtccg ccttgccgcc aggcaaccgc tccgtgtggt ggtcgacagc cgactacgta
     1861 tcccgtcccg ggccgccgtg ctgaaacggc aggaagaagc ccacaccgtg attgccacca
     1921 cgcgcgcggc ctcagtaaca aaggtcgagc gcttgcgagg ccagggcatc gatgtcctgg
     1981 tgttgcccgc tgccggcgga cacgtcaatc tcacggcatt atgggcgagg ctcggacagc
     2041 tcggcgtcac gagtgttctc gtcgaaggcg gcagcgatct caatgcggcc gtgctgcgag
     2101 cgggactgcc ccagcgcttg atgtgttttg tcgccccgct gctgctgggc gggcaggatg
     2161 cgaaaggggt gttgggaggg ctgtcaccgc gccgcctgcg ggaagccgtg ctcctcaaca
     2221 atctacgcat gacgcctgtc gggcgtgata tgctgatcca ggctgacttc ccgactcgat
     2281 aggagtattg tgcgatcttc tctccgccct gcgtacaggg ttcatccgcg gcgccccggt
     2341 ctgctcctgt ccctcgtctg catctggttg gcgctgctgt cggtttcttt gactcatgca
     2401 gccgaagagt catcctcgac ggatcttgcg gcgcaggtct ggcaatacct cacgactcag
     2461 gacagcgagc aggccaattc ggcgctggcg tccatcatgc agcggcctga tgcaacgctt
     2521 caggccgtgc aggacatcct gcgcaagggc cagccccatg gccttcagcc tgtcggggtg
     2581 ttggcggatg aacaggtcat ggttcgcgac cggccctatc gggtgagtct gtccattccc
     2641 cagagttacg agccgactaa agactatgcg ctggtggtat gtctccatgg cgcagggttc
     2701 accggtgaag cctatttgga ccgatggaag tcgcgtttag gcgaacaata tatcctggcc
     2761 tgtccaacct atcccgcggg tgcctggttt accaggcggg ccgaagatct cgtgctggcg
     2821 acggtgcaac tcgtccggga gcggtatcga atcgatccga atcggatgtt tctcagtggg
     2881 atgtccaacg gcggtatcgg cgcgtggctg atcggtatgc atcatgcgcc cctgtttgca
     2941 gggcttgccc cgatggccag tggcatcgac gatgtcttat ttccgttcct ggaaaatctc
     3001 cgcaccacgc cggtctacat cattcatggc tctcaagacc aggtgatgcc cgtggaactg
     3061 agtcgcaagc tggcgggtga gctgaagaac ctcgattacc cctacatcta tcgtgaacat
     3121 gaccggaccc atgccatggc cggcggccat ttcttcccgc gtgaagagct gcctgatctg
     3181 gtgaagtggt tcgataccca gcaccgcacg ccggttccca agaccgtgac cgtggtgcgg
     3241 gatgcgagtc atctcctgcc gttcgggtgg gtgcggatcg atgcaacgga tcagatcgca
     3301 tctttttcgg aagatctggt cgacaagcgc gacgacagca ttcggaagcg gatctatgcc
     3361 cgattgaccg cacaggtgac cggtccgaat cgggtcgacg tgcggaccga ccgcgtgcgg
     3421 cagtacacgt tgtatttgaa tgaggacctg gtcgatttgt ccaaaccggt cgtcattacc
     3481 acagacgggc aaccgtcatt cgaaggcctt ctcgaaccac atgtcgagac gctgttacgg
     3541 caggctcgcc tccggcagga tcctgcgcag ctttttcccg ctcacgtgac tgtgaccgtg
     3601 cctcaacggg cttcatgacc gggggcggcg agatggttcg gcgaatcggc ccgttggccg
     3661 ctgtgctcac cgccgccttg gctctctgcg attccaccgg gcaggccgaa ccagaacgct
     3721 gttccctgcc gtcgcaccat gtcggcatgg tgttccctct cgatcaggta gatgctgcct
     3781 ggacctgccg gcttgaaccg atcattgcgc attacaccac ggccaataag gtgggaccga
     3841 tccgtacctt gctgccggag gatgtctatc tctatctgtt ggagcatccg gtcatggccg
     3901 cggccctcgt gaatcgtctc gatctcgggt tgtacaaagc tgagtggcgc ggtcccgatg
     3961 aattctgggc gacggacggt gaaggcacgg aaggcatcgt gcatccgttg tatcaggatc
     4021 ggagcacgcg gatttactac gttgaaggga cgcatgatgg acgattcctg ccccgcgtca
     4081 gcggcaaggc tctggtgttg ttcaaattgc agccggtgaa agacgggcag ggcagcgaat
     4141 cgatggacaa tacgatggtg gcctacctgc ggctcgataa ccggttgtac tccgggttgc
     4201 tctcgatgct gcgtccgctc atcggcaatg tcgtcactcg tcaggtcgtg aaagcgtttg
     4261 atgccgcccg tcgtttggcg ggcgtcatgc gcgaccatcc ggatcaggtg ctcttcgagg
     4321 cgacggatcc ccccggtctt ccggatgaac aggtcgcgtt tctcaaagcc gccctcgcgt
     4381 ccctccacaa tcctgggcag aagccgagcc ccgcccttcc atgagaaagt gatgacgacg
     4441 tcccgcagct ttttctacct ctgtaccctc ggcgtatttt gtttcattag ctataacctc
     4501 gttcgcatgc cggtcctgtc cctcttcgcg gaatcgctgg gtgccgggcc ggagcggatt
     4561 ggattgatcg tctcggtatc gacgattacc ggggtgctgc tgaaattgcc gtcgggtgcc
     4621 ctgtccgata tttatggacg gaagatgctg ctccgcatcg gggtggtggc gttcgggctg
     4681 ccgcccttta tctatccgtt catttccgat ctcaatgcgc taacagcgtt gcggttcgtc
     4741 cacggcctgg cgacggccat cttcgcgccc agcgccctgg ccacggtagc tgatctctac
     4801 aaggagcggc gaggtgcggc gatgggaacc tatacggcct gtacccagtc cggttccctc
     4861 cttggccctt ttattggagg atggttggcc catgcggcgg ggtttccagc ggcatttgtg
     4921 acggccggtg tcttcgggtg catcgccatt ctcatcttct tcagcctcca tttggacgag
     4981 ccgcctccgc gtgtacgcga gaaggggctg gctccggtga tcaccgagat gggaaaaggg
     5041 tttctcgcag tggcccggaa ccggaaagtg ttgattacca gttccaccga tgccgccaag
     5101 atgatcgcca atggcgcgct catggcgttt ctgcccctct acggcctgtc agtcggcctg
     5161 aacgccggtg aagtcggcct gatgttcagt gtgcaggcgg tgacgtcctt tttgtccaag
     5221 ccggttatgg ggcgcatatc ggatcgggtt ggtcggcagc cgttgatcgt gctcggcctg
     5281 gtcatttgcg cggcgacgtt catcagcatt ccccacgtcg gatcattcgc tgtgctgctg
     5341 atgctgtcct ccgggttcgg gttcggcgaa gcggtggtgt catcctcatc ggccgccctg
     5401 gtggccgaca gttcggaatt caaacggttg ggggcgggaa tgggaatgca gggaacggtc
     5461 atggatatcg ggcatgccag tggcccgctc ctggccgggt ttttgatcgc ccacgccagc
     5521 tatcagggag cctttgccgt gatcgccggc atccaaattc ttgcggccgt cgccttctgg
     5581 gcgacgatga gaaccatttt gcggtagtgt ataatgccgc cgcatttgtc agggcagcac
     5641 tagacaatct ttttttcaca aggacgcagc aaaggatgga tacggatttt atcgaaacgc
     5701 ttcaacgcgg gattgccatc gtgggcgggg ttggattgct cggattttgt ctctatctct
     5761 tgaagacgca caagggggcg taagccatgt tcagcggcat tgtggaggag atgggcgcgg
     5821 tttccatcct gaataagggg ttggccggca cccggctcac catcatggcc tcgactatca
     5881 tgaccgatct gacgattggc gccagcgtga gtgtgaacgg cgcatgcctg acagccgtcg
     5941 ccagaaccga tcatgatttc tcggtggacg tgtcgcccga aaccctctcg gtcaccaccc
     6001 tcagtgatct caccaccgga tcgcctgtca atttagagcg cgccatgaaa ctgaacgaac
     6061 gaatcggtgg gcacatggtc tccggccacg tagacggcat cggcgcgatt cgcagccggc
     6121 atcaggatgg caatgcgctg gtcctggaga tagaagcgcc caaagacatc ttgcgtctct
     6181 gcgttcacaa aggttcgatc acggtggagg gtatcagcct gaccatcaac gaggtgacgg
     6241 agcgctcatt cctcgtggcg atcattcctc acacggccaa agtgacgacg ctcggtctga
     6301 aacaggtagg cgacaaggtc aatcttgagt ccgacctgat cggtaagtac gtcgaacggc
     6361 ttctgcagga tcgagggctt cttccgccga aacccacccc cgtcatcgac aaggactatt
     6421 tgcagcgccg cggcctgatt taacaggatg ctgaaaaagt tcgccagcgg cgttctcgct
     6481 tcgttcagat cctcgacgta cccgggaagg cctcgggcct aagactcttc ccggctcatc
     6541 gttccctagt acccacacat gttgagcttc aggcttctcg cgccgtgggc ttcggtactg
     6601 gtctgcctgg acggcctttt gatcatcccg tgttgccgga attggctagg acagatcagc
     6661 tacttcagca gaaacccttt ggagtcttcg ccggacgctg tggtgacggt catgatggcc
     6721 atgcctttgc gcttgctcgc ggggacggtg gcggtgattt gagtcggact gtcgagcttg
     6781 aaggtcgcgg catttccagg cccgaaggac agggatcgca gacattctgc cgggccgaaa
     6841 ttcatcccgg agatggtgac cttctccccg gcttttcctt catcgggttt gacggattcg
     6901 attttgggcg cttccccgaa acaggcttga gctttggctg ccgtttctgc cgaggcatac
     6961 ttggtcgccg gggatttgac tggggcggtg gtggtcttcc ggccctctcc ctttttcacc
     7021 gacttcttct ccggagcttt tttcgcctga gcgggagcct ggcccgcggc tttgggtgta
     7081 tcgtccgcag cgagaatggg aaccactccc ccgagtaaga tgatgcctgt gaccattgac
     7141 cacaggagtc cacgtgttgt ccgttgccct agcatgaccg gcctccatgc tttcgttaga
     7201 gatgatgctg atcaacagtg aatcagctta gtcttcaatc gtggaaatat cccccacgtc
     7261 ctgtcccaac tccttggctt tgagcactcg ccgcatgatt ttgccggagc gcgttttggg
     7321 caaggatgac acaatatcga tttcctgcgg cacggcgatt tttcccagtt ccttcagcac
     7381 atggtccttg agggactgca ccaactcctt cgaatcctgg tagccctgct tcagaatgac
     7441 gaaggctttg atcgcttctc cggcggtccg gtgcggcttg ccgatgacgg ccgcttcagc
     7501 cacggcttcg tgactcacca aggcgctttc gacttccgcc gtcccgatgc ggttgcccgc
     7561 cactttgatg acgtcgtcgg cgcggcccat gaaccacatg tagccgtcct ggtctttccg
     7621 gcagacgtcg cctgccgtat aacaattggg gatggtgttc cagtagacct tgtagcgatc
     7681 cgggtcttta taaattgtac gcatcatcgc gggccagggc ttcttgatca ccgcaaagcc
     7741 gccggcattg gcgggcaggc tgttcccttc cttgtccacc acgtcggctt caatcccgag
     7801 gaacgggcgc gtcgccgaac cgggcttgag cgggacagac gggaggggcg tgaccaggat
     7861 cgcgccggtt tccgtttgcc accaggtgtc catgatgggt ttgtcgctgc cggtcacgcg
     7921 atagaaccat tcccaggctt cgggattgat aggttcaccg acgctgccga ggatgcgcaa
     7981 cgtggacagg tcgtacttct tcggccactc ttcgccatat ttcatcaaca gccggacggc
     8041 ggtgggcgtc gtataaaaaa tggagacgcc atagcgcgaa atgaggtccc accagcggcc
     8101 ggggttgggg taatcgggtt tgccctcggc catgaggatc gtggcgccgt tcaatagcgg
     8161 gccatagacg atgtagctgt gcccggtcac ccagcccggg tcggccacgc agaaatacac
     8221 gtcctcttct ttcagatcga acacgtattt cgtcgtgatg tatgtgccga ccatgtagcc
     8281 gccatggacg tgcaccacac ctttgggctt gccggtggtc ccggacgtgt agaggatgta
     8341 aagcggcgtt tcggcatcaa gtggttctgc ggcacagacg gcgttctgct ctttcagcca
     8401 atcggcccaa tcgatttctt ttggcgcggt gagtgcaacg ctgagggcat cccggcgcac
     8461 cacgacgact ttttcgacgg aggggcagga ttgcacggcc gcgtcgacca cgggtttgag
     8521 cggaatggtt tttccgcggt cgtacccgac gtcggcggtg atgacgagac gcgcttccgc
     8581 atcctgaatg cggcttgcca aggccggcgc actgaatccg gaatacacga cgctatgaat
     8641 gatgccgagg cgggcgcagg ccagcatggc cacgacctgc tcgggaattt tcggtaaata
     8701 gatcgtgacg cgatcgccct tgttcaagcc aagggctttc agggcattgg cgcagcggtt
     8761 gacctggcgc agcagctccc cataggtgaa gatgcgttct tcaccattct cgccgaccca
     8821 gatgatagcg accttattgc gacgccagcc gttcgtatgg cgatcgaggc agttgtaggc
     8881 aatattacag gtcgcgccga cgaaccattt cgcccagggg tagttccact cgagcacccg
     8941 gctccacggg gagaaccatt ccagctcctt ggccacgccg ccccagaagg cttcgggatc
     9001 ggcaatggaa gccttgtacg cctggtcgta atccgggatg tgcgccgccg cttttatccg
     9061 ggcggacggc tgaatgacac gtccctcttt caatagcgta tcgattttct cactcatgag
     9121 tcgccatacc tccacaaaac gcgggcctgt ccgatgcgcg gttcaatctc tccggcatta
     9181 tgcggatggg gcggaggaac tgcaaccctc aagccgcaga gaggtgccgc tggagcgcag
     9241 cgaatgaccg cgtgggacaa gcgttggcgc cgatggcctt tcttgacagt cgggacgacg
     9301 cgagggtagg ctgagtccgt tacccttcac tggatcatcc tgctgatgcg ttcgccgaga
     9361 tctctcacgt tcccccggat gatcacctgc ctggcggcct tattcgtcgc cggaatgtcc
     9421 actgatcatg cggctgccca ggtcgggaag ccggagggac tctattacaa atcatgggcc
     9481 gtcgtcgtcg gcattgagaa ttatctcgtc gcgcccaagg tgccgggagc gctggagagc
     9541 tcacatgcag tcgctgaagc gttgcgaggg ctcgggtttg atgaggtcat cgaaatccag
     9601 gacaaggagg cggcctccaa acggctgctc catatcctca acgattacct gccgcgcaaa
     9661 gtgggtcggc aggatcgcat cgtcttcttt ttcaccggcc atgccggcct gacgcaggat
     9721 tctcaagcca aggattcggg cttcgtggtt ccatgggacg cgcagctcaa caatcccgcc
     9781 aaagccatta ccctggatca actcaaagag ttcagccggc gatcggcgtc caagcacatc
     9841 ctcatggtgt tcgatgccaa cctgcgcggc tgggacgtga ccgcgccgca gccgctctcg
     9901 ctggaagggc gattgtcgcc ggaagatgac accgagaaac gatcggtgca agtactcacg
     9961 gcagccgaga aagaagaatc agttgttcgt acgtcggggc agagcgcatt cgtgacggcg
    10021 ctagtggccg ggctgaaggg tgcggcggac ctgaataaaa acggctggat catggccagt
    10081 gaactcgcgg ctcaggtgaa gcaggacgtg gagcgtgaga ccaagggggc ccaacatccg
    10141 caactccttc aacttgaggg agacggtgat gtcattctgg tcgaagggcg gaaggccatg
    10201 ttttccgtcg gcaaggaacc cagtaccgaa gcagaccgga tgaaggaagc ccgagttcaa
    10261 tatgaacagg catttgcttt attgcagcag cagaagtcgg cagaagaagc gttggaacgc
    10321 ctcaatcgcg cgatcacgta cgatccgtcc ttcggcgacg cgtatgtgtt gaagagtttt
    10381 atccgccttg aggtcctgcc caacctcgaa gaatcgctgg cagctgcaca ggccgcggtc
    10441 cagcatgcgc cgcagaatcc ggattctcac tactcattgg cgctggccct cgaaaagaaa
    10501 ggccagcacc aggaagctga gcgggcgatg ttgcaggcat tggccgtcaa tcccgcctat
    10561 accgatgtct atttttcact gggcaccttg tacgccgacc acctgaatga accccagaag
    10621 tcggtcgatg cctttcgccg atatttggaa ttaggcggcc agagcgagcg cgccacccgc
    10681 gccgttcagg gtgatcgtgc tcccggtgag aaacccgctc cgtagcagca gtcttgaccc
    10741 gcctaccacc tcgtcagact tgattgagcg tgttgtgccg tgaggggtcc gacacctatt
    10801 tcttctcact gaacggctag tccttgcctc cgcccttcag ataccccagt ccgatcttca
    10861 atgcccggcg ggtgatctcg ggccagcgta cttggggata ttccgccagc actgcggctg
    10921 ccttcggatc ctgaatatcg agttgaacga cccgaatgat gccgtcttca atctgaacgt
    10981 cagccatctg tttcatctcc ttcgcgtatg ctacgtaaaa atatttcctg tcgcaggtcc
    11041 agacgtcaac cgcaaaaccg gttgcgcgtt gacagtcttg ggggtgcatg ttagcataaa
    11101 aactcgcttt ttcgcgccat tgtctctcgt cacccagtga accacacacg tcgatcttcc
    11161 atcatcttgc cgcatacaac cgagccatcg gatctcacag gtgccacacc aaggaggaat
    11221 cgtatgagca gagtagaggg ggctttctcg aagttcaccg gaagcgcgtt gatgctggta
    11281 ctcctcatcg ggctgacggc ctgcggtggt cccccgaact gggtcaaaaa gggatccggc
    11341 gccttcaatg aaaaaagcga taaatccttc tatggcgtcg gctccgtggt gggtgtgcgg
    11401 aacgaaccgt tagcctggga cacggcggag aaccgcagcc gggcggaaat cgccaagacc
    11461 tttgaaacct ataccgcgta tctgatgcgt gattatgctg cctccacgac ggcgggcgat
    11521 ttttctcgca acagtgaaga gcagaacatc gagcgggcag tgaagacctt ttccgcggtc
    11581 acgctgaacg gcgtcaagcc catggaccga tataaggacg aaaagtccgg cacgtattac
    11641 gtcctgacca agctcagcct cgaagacatg aagaataatc tggagcaggc caaagaactc
    11701 aatagccagg tccgtgactt cgtgcgtaag aatgccgacc ggttattcga tcgtctggaa
    11761 aaagaagaag acaagcgggc gacgaaatag tcgaggcgtt atccgggtct gtggccggga
    11821 ggagtcagca tgatcgagtg gcgagagcat tcagtgttgc gaacctgtgc ggtggggcta
    11881 ttggtaggat gcgcggccgt agtcagcggg tgcggccatg agacgaaggt gacgcgggtc
    11941 gatacgggca cggtcactga ccttagcggg cgttggaacg atacagactc ccagatggtg
    12001 gctgaggcca tggtgaagga agccctcgcc aatccctggc tgggcaactt taccaagggc
    12061 aagaatcgtc agccggttgt aatcgtgggg acggtgctca ataagagcca tgagcatatc
    12121 aacgtgcaga cgtttgtgaa tgacctggaa cgtgagttga ccaactcgca aaaagtcacg
    12181 tttgtggctg ccaagggcga acgtgatgag gtgcgggaag aacgccgcga acaggctgtg
    12241 catgcccgcg aagatacgca aaaagcgccg ggcaaagaaa tcggcgcaga ttatatgatg
    12301 cggggaagta tcgccacgat tcttgacgaa caggacggag cgaaagcggt cttctatcag
    12361 atcgatctcg agatggtcga cctggagaac aacgtcaagt cctggttcgg ccagaaaaag
    12421 atcaaaaaag tcatcgaaaa gaaacgcacg attttttagc cagacgtatt cagttgagac
    12481 ctcacctccc atcgaccacg gcctcccccc agggaggccg tttcatttct tggcccttgc
    12541 actctgtcat cgggtggtgc ctgctgtggg tgctgtccgg ctgcggcctg tcatccaacc
    12601 attatctcct catcgatcaa agcctggcgg cacacgacgc tcgacgggcg gatgcgatca
    12661 tggcggaggc ggagtcggaa tatggacccc gcaatcgtct cctctacagt atggaccggg
    12721 ggatgaccct tcacctcgcc ggcgagtaca tgcagagcaa tgcccagctt gaatcggcgg
    12781 cacaggaagt cgaacggctg tatacgagga cgatcaggac cgaaaccgca gccttcctga
    12841 ccaacgataa cgtgctgccc tatgaagggg atccctacga acatgtgatg atcaacatca
    12901 tcaaggcgct gaattacgct gcgatggggc aactgatgga agccgtcgtc gaggccaggc
    12961 agatcgatca tcgcctgaac gtcttgtccg atgccgccaa ggaaaaagac gggtaccgtg
    13021 aagatgcctt tgcccgttat ctgacgggcg tgttgtacga ggcgaccggc gatctgaata
    13081 acgcgttcat cgcctaccgg aaggcctacg acatctacga tgcgacgaag agctgggcgc
    13141 ggacgcccat gccgccgatg ctccgcgcag atttactgag gacaaccgat gcgttgcata
    13201 tgacgaccga gttcgaagaa tatcggcgcc tgtttcccga gacgcgttgg ctgcctcgcg
    13261 ctgaccaaca gaatctcgcc caggttgttg tgatcggcta taatggccgc gcgccgcgca
    13321 aggaagacgc gtttctcgac atccctatca gtctgagcgc gctgcagttg gtcttgttga
    13381 atcgcggtgt catccatgcg tcgaatcgtc aggagcgccg cgtggcggac agcgtgttgt
    13441 atggcttgaa tgggcgagtc gtacgggtgg ctctgcccaa actgatcctg cagaaaacgc
    13501 aggtggtgca cgagacggtg acgttgatcc ctcgaggagg agagccgatt tctgcgacat
    13561 ccgagttagt ctacaacggc accgccctgg ctgagcggtc cctctccgac cgcttacctg
    13621 gaattacgac gaaagccctc gcccgggcag ctgtgaaatt tgccgcggct gaagccgcaa
    13681 cccgaggatc gcagcatgcg gtgaataaag gcgacgcgcc gtgggtcgga ttactggtgg
    13741 gcgtcctcgc gcatggattg gccgtggcgt cagaatccgc cgacaccaga agttggcgca
    13801 ccttgccgga tgagattcaa atttcccgcc tgtgggtgcc gcccggcgaa tatgagagcc
    13861 ggattcaagc catggttcga ggcaacggaa cgccgcaggt agaagggccg cgccctctcg
    13921 ttcttcgagc gggacaaacg ctgtttctga tcgaacgagt gatgctgtga gggacgctga
    13981 agccatggtc tcggatcgag gcacgcgaat cagcctagtc gctctggtca tgctgctgtg
    14041 cgccgggtgc ggctgggtcg gtgggccatc ccgtccatcc tggatagacg gcagcagtac
    14101 gcagtttcct tccacgcaat atctcgtagg cgtcggccag gccgattcgc gcccacaggc
    14161 gacggaacag gcctatgcgg cggtgtcgcg catctttaag gcggagatta cggcgcaggc
    14221 caaggattgg gaatcgtacc tggtggtcga atcccgcgga cagacaagca ctcagcgcaa
    14281 gttgattctg gacaatgtca cgcgagtgac cacggataag gtgttggaaa acgtccaagt
    14341 cctcgatacc tggtttgacc aaaaggcgcg acagtactat gctctggcag gcatgaaccg
    14401 ggcgcaagcc gaatcggcga tgctggaacg tctggccgac ctcgatcggt cgattcagac
    14461 ggaagtcacg gaagcgcatc aggcccagga caagctctca cacgtgcgca atctgaagcg
    14521 agctgccaaa aatctcgtcc ttcgcgaagc ctacaacacc gacctgcgca caatccggtc
    14581 gaacgggcag gggcatcccg ccgcctatcg tgtggccgaa ttgactgctg aattggagca
    14641 ttatctcgcg gcgaatatgg ggatggccgt ggaggtgtca ggtgagcagg cggaaccgct
    14701 cgagcgggcg ctcatcgagg gcctgacgca ggaagggttc tcggtcgtcg gctctggccc
    14761 aggagcggct acggcggaat tgctgatcac cggaaccgtg cggatgtggc cgatccaagt
    14821 ccctgatccc cagttccgct acgtgcgatg gtgtactgat gccgtaattg aagaagtctc
    14881 gacccatcgt gtcatcggtg cggtatcaaa aggcggaaaa gaagggcatg tgacggaacg
    14941 cgaggcagcg gctaaagcgg ttcgcgtcat gcagcaggag ttttcagcag acctggccca
    15001 agcggtggcc gcgcatatct acggagagag ggacttatcg gtgtcatcct ctgcgccgtc
    15061 aggttgtccg aaagaggcgg ggcagccgcc gctcagtcgt taacggccgc gaattcacca
    15121 tagagagagg agagggaatg gcaaagacag ggacccggaa ggcgggcgaa caggccggcg
    15181 gcggacgccg gatgtccagt caggcaatga aaaaacatct gcgggaacgg ctcaccgccg
    15241 atacgcagca ggtgctgaag agcgatattg tgagcatctt tcgcaagcaa ggctactatg
    15301 aggaattgcc gttggatgag ctgcaggatc gcctctccaa gctccagtcg ccgttggccg
    15361 acagtctctt gaaggggagg gtgtaggatg ccgtattact attttgattc cacggcgctg
    15421 gtcaagcgct acagcatgga gcgggggacg cgtatcgtca acaagctcat ggtgaagcgg
    15481 gggaaggtcg ccatcctccc catgtggagc gtcacggatt tttattcggc cttgtccgtc
    15541 cgctcgcaac agggagagat cacgcgggac gattgttatt cggtcttata caagtttgag
    15601 atggaagcct cgcaaggact cttccagttc atttcgccga cgatggacac ctatctggct
    15661 gcgaaggaat tgattttgga ctatccggcc cttcgctcgc cgcagcttct gcacctggcc
    15721 ttagccctgg agttgaagcc gctccggctg accgtcgtca gcgcagacaa acaactgctg
    15781 gccgcctgtc gtcctgccgg gttgcacatc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nngagcgatg cgagaacgaa gctggcggtc tttttcaacg gcctgctagt
    15961 tggggcagcg aaattcgccc atcatcgatg ccagcacgat ggcccaatcg tctgctgttt
    16021 gacggatggc gggcagtttt ggtgagaccg caccaggtcg gtctggagtc tccgatgagg
    16081 cgagggtctc ctgggcatgt tgtagctcaa gctgtgccag ttggaggctg ccgcatacgc
    16141 cggcagaggc gggaagttct ccaccggccc gcctgaagag agccagcccc cggtatccat
    16201 gctcctgtga tcgatacagg tgttctgtgg atttgaatac ttgccgcaac gccggttcag
    16261 tctgacgttt cccgagttgt gccgccatta gtgcggcgcg gcccatgttc atggccgccc
    16321 cttccgcatc atcattcccc atcgcgtctt ctgctttggc tcgtagccgg tcgagctccg
    16381 cctcttcgcc catcacttga gcctgtccga ttccggcggc ggccaccagt atgaagaagc
    16441 taaggattga tgcggcgagg caatgctcgc accggccgat gtgtatgcca ttcctcactg
    16501 tacgtgttcc caggtatcga tttgcttgat gctccaattg accgcttcct tcaatttgac
    16561 gagattcggg tcggcatcat tttcatggac gtggtacagc gatttctgaa gggcgaagag
    16621 cggttcgtag gcccgcatat ccggcaattt tccgagcgcc caggcgacct cggttttcac
    16681 ggcgacatct tcggtgttca gggtttcaag cagagggtcg acgatggtga agtctccatg
    16741 cagcgcaccg acaattccca acgcctttgc cgcgggggct cgtaaatcgg gcgcaccgtt
    16801 tttcatcgtc ttgatgagga cggggatcgt atctttctgg ccgatattgc ccagggccag
    16861 tgcggaggct ttcgcgatgc tccgatccgg cccgttgagg ccatccagca agcgcggaat
    16921 ggctttcacg gagaagaggt cgccgaacag cgccactaac ttcaccttgc gtgccactgg
    16981 cgttttcgga tcgtcgtaca gacgaaacag ccgtgcttcc tgtccccatt cagcggtgat
    17041 gagggcagga aaatcgtact cttcgaggcg ggcttgtaac ctgcccatgg caaaggacgt
    17101 gggatcgccg gccttcgcca gtgttgccgc agcctgcgca tcatcgaagt ctgtgcggac
    17161 ctccttgcgc caggccattt tcttaccatt aagcagatag ccgagctgac aggccggtcc
    17221 cttccagata cgggatggcg catcagggag atcggcgtcg cttcccatcg tggtcgtgcc
    17281 ctcccaggtc tttttctgtt cacatttgat cttcaactcg acatcgtgcg gctgggtcgg
    17341 gtcggtcacc ggcgtatagc cgaattcctt catgcgattg gccgcaacct cggcgagcgg
    17401 gccggcatcg acagagccct tgtcggaaag agcgaggacc gtgatcaaca cacggtcgat
    17461 tcgttccatc tggctttttt gctcggcggt cagggcctcc cggcgggccc agctcgggtc
    17521 gccgaacagc aggaggactc ccagaatgac aagcgagagg atgcatctca tgtcggctcc
    17581 attctgctca aaaagacgat gatggctgtc ctgtcaatca ctctataaca accccttccg
    17641 gcggttgcaa gttgcgccgg cttgaacagc ctccgtataa tcgcgccatg ttgactggac
    17701 ggcagatcgg ctttgtcggc ggcggcaaca tggccgagtc gttgctggcg ggcctgttgc
    17761 ggaaaggcct gtcctcgcct ggccgtctgg tcgtcagcga cccgcttgct tccagacgcg
    17821 aactgttgag ccgtaacctg ggcgttagtg tcacgcctga caatcaggcg gcggtccgag
    17881 gcgcggacat tgtggtgctc tgcgtggaac cgcaggtgct cgatgatgtg ctgagagaac
    17941 tcgcgccctc gcttccgtca gacccagtca tcatctccgt tgctgcaggg tatcccttgt
    18001 ctcgcgtgca acaccatctt ccttccgcca ccaggctcat ccgcgccatg ccgaatactc
    18061 catccgcgat cggcgagggt gtaacggcga tgagtcttgc gccgggggtg tcgtccgagg
    18121 atcgtgagac ggcccgccag ttgtttgaat cggttggtat ggctgtcgtc gtagaggaac
    18181 ggctgttaga cgctgtgacg gccttgagcg gcagcggacc agcctacgtg ttcgccatga
    18241 ttgaagcgct tgctgacggc ggggtgttgg ggggtctgcc gcgatccacc gcacaactgt
    18301 tggcagcgca caccgtggcc ggcgcggcca gcatggtgct ggagcagggc gagcatcctg
    18361 ctgtactgaa agaccgggtg gcttcgcctg gcgggaccac gattgccgga ctgtatcagt
    18421 tggaacagag aaagctgcgg gccaccctga tgtcggccgt tgaagcggcc acgcaacgct
    18481 cgcaggaatt gggcaagacc gagaaccatc atctctaaca aggagcgcgt ctcgtgcaat
    18541 attgggtgaa agtcgtattt gccgataatc aggaactgct ggtcaaggac gccatccggc
    18601 ataccatcag tgaggacatg gaagtgcttg aggtggattc ggccaaggaa gtcatcattg
    18661 tgccgatgaa gcagattaag tacatcgc
//
LOCUS       DAOQEW010000064        18372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_782_length_18372_cov_8.532675, whole genome shotgun sequence.
ACCESSION   DAOQEW010000064 DAOQEW010000000
VERSION     DAOQEW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_782_length_18372_cov_8.532675"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..835
                     /locus_tag="PLS35_08860"
     CDS             80..835
                     /locus_tag="PLS35_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNI18812.1"
                     /translation="MKFVCLNCETYMTFQKVEKPGEGSLGVFFGCPSCEAKFSMVTNP
                     GETQMVASLGVKLGGRTETAEPFEMTRGTLKDEAQAGAGQMAAYLNEKIQGGQPATAA
                     PKATAPAAGGEKTSGGCPFSAMVAEMGLTSGGKPQNGNGTSEFTWTADAKEKLERLPS
                     FVKPMVQSSVEAYARKHGYKSITLQVMDDSKNDSPNGIAWTKDAEQRMDNIPDFIRPM
                     ARREIERIAKERGLVEITAQVMDEAKEKFMKFM"
     gene            982..1689
                     /locus_tag="PLS35_08865"
     CDS             982..1689
                     /locus_tag="PLS35_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18813.1"
                     /translation="MVLASTGSFSIAESDDHGHSSTENHGHHHESSPSASQWEGSREG
                     IAYSEFNHHLAGVFLLLIGLSELRQALGWPVLTWTRFLLPGALAVAGLFLLIWSDHDA
                     WPIGSMTFAETFFGKDPEVLQHKTYGVLALAVGTIEFLRRLGRSATAVWTAPLPIFAV
                     VGGFMLFSHSHGDHPAADKIALHHLMMGTMAIIAGSSKLISGWRQRQLATERSYWELV
                     WAILVLVIGVQLLFYSE"
     gene            1833..2588
                     /locus_tag="PLS35_08870"
     CDS             1833..2588
                     /locus_tag="PLS35_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNI18814.1"
                     /translation="MGGHSHWATIKRHKSAVDAKRGKVFTRIIRELTIAARSGGDPDG
                     NPRLRLAIAKAKEANMPGDTMKKAIQRGTGELPGVTYEEFTLEGYGPGGTAVLMEITS
                     DNRNRTVAEIRSLLTKNGGNMAEAGAVAWQFHKKGVLVVEKGKVEEDALLTIALEAGA
                     EDVKVSDKAFEVLTSPHDFEAVKKALSDAKIEYTLAELNFIPQNTIALEEKAAEQMLK
                     LMEILDEHDDVQKVHANFDISDEVMEKVAAATA"
     gene            2670..3278
                     /gene="ruvA"
                     /locus_tag="PLS35_08875"
     CDS             2670..3278
                     /gene="ruvA"
                     /locus_tag="PLS35_08875"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNI18815.1"
                     /translation="MIALLTGLVAFKAPSQVTLDVHGVGYEVLVPLSTYYSLPNQHEP
                     VTLSIHTHVREDAIQLFGFFTASEKEAFLLLTGISGIGPKLGLSVLSTLSVRDLYAAI
                     QAGDIEKLGTVPGIGKKSAARIALELKDKVAKLHPSGDLGVAGSGRSDNPLYEDALSA
                     LVNLGYRQPDVKEALKRVEQSGVLTQGLEQVIREALKDLAKG"
     gene            3282..3788
                     /locus_tag="PLS35_08880"
     CDS             3282..3788
                     /locus_tag="PLS35_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18816.1"
                     /translation="MVMMELARKKQDRPGIAMALGVVAVLLCASMTDRVFAADGMDEP
                     KSIRKTCGSCPEGYATTGRTNAPEVCKDGDPVLVQCVPLGSNMLSVCGSCPEGYSEVG
                     RSNVPSRCGSVDGGLVSQCQSQQMGSSMPDPSQGGVRCPPDCGSTAAPGQGASPPPPK
                     FRPAPETR"
     gene            3818..4855
                     /gene="ruvB"
                     /locus_tag="PLS35_08885"
     CDS             3818..4855
                     /gene="ruvB"
                     /locus_tag="PLS35_08885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNI18817.1"
                     /translation="MADRTVSNQLTDDERGLEAALRPQSLREYVGQPRMKESLEICIE
                     AAKRRGEALDHAIFYGPPGLGKTTIAHIIAREMGSAIRSTSGLVLSHAGDLAAILTNL
                     QERDVLFIDEIHRLPASVEEALYPAMEDYQLDLVVGQGASTRTVKLELPHFTLVGATT
                     RAGALTSPLRDRFGLVHRLEFYSSQELTSIVTRSAGLLNIPIDEAGAAEIARRARGTP
                     RIVNRLIKRIRDYAEIKAGGRITQRVAQDALVWLAVDEAGLDEMDRRVLLTVIEKFHG
                     GPVGVDSLAAAVQEDKGTLEDVYEPYLIQAGFLERTGRGRQATRLAYEHFKKQKDLLP
                     FSSETDSIATP"
     gene            4995..6071
                     /gene="pheA"
                     /locus_tag="PLS35_08890"
     CDS             4995..6071
                     /gene="pheA"
                     /locus_tag="PLS35_08890"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNI18818.1"
                     /translation="MSDDLQVHREEIDRVDDQILRLLNERSKSVIEIGRLKKLKDADA
                     NLHTQARESAIFDRLSKQNTGPFPTEAIRAVYREIMSASLSLEGPQKVAYLGPRATFT
                     HMACMQKFGASAQYIPVNSIKDVFSEVERGRAHFGVVPIENTTEGVVNHTLDMFVDSN
                     LLIYGEVLQEVAHHLMSKSGLAGDIKRIYSHPHAIAQCRNWLETNLPHVPVSEVASTA
                     RAAEMSVDDPSAAAIASELASQLYGLKVVTARIEDNINNFTRFLVLSQKAPERTGRDK
                     TSLMLSVKDKVGALYDLLRPFASHGLNMTKIESRPSRRKAWEYIFFVDIEGHIDEERV
                     KKAAEEVKSRCLFMKILGSYPAYS"
     gene            6088..7173
                     /gene="hisC"
                     /locus_tag="PLS35_08895"
     CDS             6088..7173
                     /gene="hisC"
                     /locus_tag="PLS35_08895"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNI18819.1"
                     /translation="MPLKVHPDIVSLVPYVPGKPIEELQREMGLPRVVKLASNENPIG
                     PSPKAMAVLAETVPTLHRYPDGGAFRLRGALAERWKVAPDQVILGNGSDEILGLLART
                     FLSPGDEAVMAEHTFVIYKMEVQAAHGVAVEVPQKNWHHDLPAMAAAITEKTRLLFVC
                     NPNNPTGTMATKAEVAALMERVPDHVVVVFDEAYYEYVRHSEFPESLAYVKAGRPVIV
                     LRTFSKIYGLAGLRIGYGITTPEITNYLNRIRPPFNANSMAQRAALAALDDDAHVAAS
                     RSLNHVEMDKVRAGLRRLGFEALPSETNFLYFDVGRNGRAVFDALLAKGIIVRHIEGR
                     MLRVTIGLPDENQLFLSALEDVTRASQ"
     gene            7192..8205
                     /gene="aroF"
                     /locus_tag="PLS35_08900"
     CDS             7192..8205
                     /gene="aroF"
                     /locus_tag="PLS35_08900"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNI18820.1"
                     /translation="MIIVLKPDASEREVDHITDRLRELGLKSHISAGQERTIIGVIGD
                     DRILHNQPLTALPGVESVLPILAPWKLVSREFKKENTIIDVNGVKIGDKKITIMAGPC
                     AVERLELTVGIAHEVKSAGATVLRGGAYKPRTSPYSFQGLGREGLDYLVEAKKQTGLP
                     VVSEILDTRDIELFLEKADIIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYVMSRGNRNVMLCERGIRTFETQYRNTLDLAAIPTLKDLSHLPVIVDPSHATGK
                     WDLVAPMSKAAIAAGADGLLIEVHSNPECALCDGEESIRPSKFKELMGDLRKIAAAVD
                     RTL"
     gene            8261..9157
                     /locus_tag="PLS35_08905"
     CDS             8261..9157
                     /locus_tag="PLS35_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNI18821.1"
                     /translation="MTTPHFKQAAIIGVGLIGGSLGMILRRQKLADSVVGIGRRVENL
                     KTAVEVGAIDRYVSDPREGVEQADFVLLATPVDTYERHLQEWAGCLKPDTIVSDVGSV
                     KGELVTRAEALMPSGVKFVGAHPIAGKEKTGVAAGSETLFSGARCILTPTTHTDPEAL
                     KVVRKIWELAGSIVLEMDPFLHDKILGAVSHLPHVAAFALMTALADVRDHGVPELDLA
                     GHSGGGLRDTTRIAASSPEMWRDIFLWNRDNVVSLIETYERHLGELKRLIAAGDAAGI
                     EKQLDKAKYEREQLIPRTPGKA"
     gene            9164..10492
                     /gene="aroA"
                     /locus_tag="PLS35_08910"
     CDS             9164..10492
                     /gene="aroA"
                     /locus_tag="PLS35_08910"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNI18822.1"
                     /translation="MASLTITPGRPLKGTITVPGDKSVTHRAIILTALAEGSSTVKEY
                     CRGEDCLNTMRAFQSLGVRIEETPESLTVYGKGMWGLTEPFGPIDCGNSGTGIRLMAG
                     LLAGQDFFTVLTGDESIRRRPMGRVVKPLRAMGATIAGRKGGELAPLAITGTRLKGLS
                     YVSPVASAQIKSSLLFAALYADGLTTISEPRLSRDHTERMFAYFGIPFERDGCTVRIE
                     GRPSVRWSGKPVLVPGDLSAAAFFLVGASIVPGSDVTVRNVGMNPTRTGLIDILREMG
                     AHIEVLNPREEAGEPVADLRVLSRPLHGVRIGPDQIPQTIDEFPILCVAAAVAEGETV
                     VTGAEELRVKESDRIATMAKELRAMGARIEERPDGMVIQGLGHNGANGALAGAICESH
                     GDHRVAMSVAIGGLTAAQATQIQNTACIETSFPNFECKLLELLTDSGKRL"
     gene            10505..11203
                     /gene="cmk"
                     /locus_tag="PLS35_08915"
     CDS             10505..11203
                     /gene="cmk"
                     /locus_tag="PLS35_08915"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNI18823.1"
                     /translation="MGEIGVASRGKRGLIIAIDGPAGVGKSTVARLLALRLGYLYLDT
                     GALYRAIAWKVHNAGLSPDDHLAIAALLPKTTLQMACGPEQSHVLLNGRDITGELRTP
                     AVSALASMVSAIPAVREWLLPVQRQIGAEGCVVAEGRDIGTRVFPEADVKFFLEADAE
                     VRATRRHRELVAAGHSVQFDQTKRDLTGRDDRDRTRAVAPLIPASDAERLDTSSLPVE
                     EVVSHMMAVVAARL"
     gene            11200..11895
                     /locus_tag="PLS35_08920"
     CDS             11200..11895
                     /locus_tag="PLS35_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNI18824.1"
                     /translation="MSSALYGLLWVLSRIIGWLCFRYRTIGQVPRKGGVLIASNHASY
                     LDIPLLGCGVPRRVWYMGRHDLFPVPLLNGLLQALGWIPLRIGRLDRDAFSKAVALIK
                     EDKAVAIFPEGGRTMTGALKPGKPGIGVIVSQTGCRVVPAHIGGTFEVLPPGAKWPRF
                     RRVTVTYGEPLDFSADAAQLEGKVFYQHVSRTVMAKIAELGQVPNPGEGQAHAGLTHD
                     REATPTAKSCNAE"
     gene            11950..13665
                     /locus_tag="PLS35_08925"
     CDS             11950..13665
                     /locus_tag="PLS35_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNI18825.1"
                     /translation="MSTATPQSEPKLDHDALAAMYEETFRNFEEGTITEGMVVAIGKD
                     KVVVDIGYKSEGMIPADQFSQEELGQLKVGDRLQVYLEECEDADGNLVLSKEKADKMK
                     IWEELEKLHKEEKSIEGKIISRIKGGMMVDIGVKAFLPGSQIDLHPVRDLDGLVGKTF
                     PLKIIKINHRRGNVVVSRRVLLEETRDRRRQTTLSTLKEGQLIQGTVKNITDYGAFID
                     LGGIDGLLHITDMSWGRVGHPSELFQVSDKVEVTVLKYDRETGRISLGLKQKSADPWT
                     GVAAKYPVGTRVRGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSRVVSVGD
                     QVEAAVLNIDPGSRKISLGMKQTAPNPWDMIEAKYPAGTRIEGKVKSLTDFGAFVGLE
                     EGIDGLIHISDMSWTKHIKHPSELFKKGQKVDAVVIRIDKEKERLSLGYKQLSRDPWE
                     EQIPNKYRVGDSITGKVSKIADFGLFIELDGDVEGLIHISEVGLDANVRMEEKFKPSD
                     DVTAKIIKVDRDERKIALSLRDHQLDSDRRQVDEFHASQGGIDQTLGRAAKQSRKRNQ
                     SDSEA"
     gene            13681..14580
                     /gene="sppA"
                     /locus_tag="PLS35_08930"
     CDS             13681..14580
                     /gene="sppA"
                     /locus_tag="PLS35_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNI18826.1"
                     /translation="MGPQADTAGKPQRSLLRRILWAIVIVGGALILLNALLPDLDFST
                     QDRVALIRIEGVILDAQATISELKQYSENPLVKAIVLRIDSPGGGVVPSQEIHDAVKR
                     VKNKSNKAVIASMGTVAASGGYYIAAATDRIIANPGSLTGSIGVIMEMANFEGLMKKV
                     GVEGVVIKSGRFKDVGSPLRKMSDEERKLLQSVMDDVHQQFIQAVADGRSLEVSDVEP
                     LADGRIYTGRQAKEARLIDELGDLDDAIHIAADIAGMEGEPKVVEPRKRFSFREIIES
                     RWSSVFPKLELNTGVKLKYLMAF"
     gene            14656..14934
                     /locus_tag="PLS35_08935"
     CDS             14656..14934
                     /locus_tag="PLS35_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNI18827.1"
                     /translation="MTKAQIIERVSEQVTTLTKRQAEIVVNTIFDCIRDSLRSGNKTE
                     IRGFGSFRLRARRMKEGRNPKTGATVAVPAKRVPFFKAGKELKELLNK"
     gene            14948..15943
                     /locus_tag="PLS35_08940"
     CDS             14948..15943
                     /locus_tag="PLS35_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNI18828.1"
                     /translation="MAESSNITAPEVRWSDGALKRMERAPMFLRGMVRRLAEKKAREL
                     GYAEITEEILEQFKGQMMGTMGGEGGMAEAADQMAKGQLPWTAAAKERLAAVPEFMRG
                     MIKQIADEIARKGGHMEVNVDLFEKVEALGEIHERETTPLEWTEGALALLQQKIKESP
                     PIAMDFVSDMIRHDTEEMAREKGLTRIDEQTAVQLWEAPQERVAWSDEAWKRLQTSPD
                     FVRSGIRKAAERRARKLGLKEVDSEHLTTFRNQAMMKAVKRIRSFGYNELTFDAFDTA
                     LQKTKRLQGNDQAEKRLQEIRSHFSDPEVKKPEGGTLGADLMGRFRKFLKGEGSL"
     gene            complement(16275..16601)
                     /locus_tag="PLS35_08945"
     CDS             complement(16275..16601)
                     /locus_tag="PLS35_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18829.1"
                     /translation="MSKKVTIPLSMYGVAEVLKWCIDRNNGRVPGVDTEGFKRMQSLL
                     AEKPTSNDYLALDQFWKKQVALELTEDEVSTVDRCLYDIPNFDNEPLPQIRHKFWPQA
                     AGIAAH"
     gene            16850..18043
                     /locus_tag="PLS35_08950"
     CDS             16850..18043
                     /locus_tag="PLS35_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNI18830.1"
                     /translation="MTINTYANKRRAIGRVGLALIAGLPLLFACSKTDPYVPPDPFYY
                     FESYAVGKNPTTVTTADFNRDQITDLITTNISSNSISLLFGNGDGTFRDQVQVKVCQE
                     PRSLALNDFNHDDQLDVALACSGGDQISILFGRGNGKFDEGPQYPVHRAPVSISSQDV
                     NGDGHPDLAVALRNDKVKIFLGSARGEFRPGVQYEYGDTPTSVALKDLNQDGQPDLVV
                     TNGGPMLSAVSVWIGNGDGTFRDPKDYKTGRRPLGVSFADFNNDHLPDLLVINGEGDS
                     FTTFLGNGNGTFQPGKDSGADASPNFGVAHDFDGDHIDDVAIVNLQSTDLSILFGRGD
                     GTFHYPPRNYRTKNGPFAVASYQVSADNGEEPGLVTADNGAASVSVFLHHGRKGQIPP
                     NSKPE"
BASE COUNT         3989 a         4938 c         5670 g         3775 t
ORIGIN      
        1 ggcggattca atcgttgctg gattacaaaa aagtcctggc ggaaaagctg taagcagacc
       61 ggcctggaag ggggagccta tgaagttcgt ttgcctgaac tgcgagacct acatgacgtt
      121 tcaaaaggtc gaaaaacccg gtgagggctc actcggtgtg ttcttcggct gtccatcctg
      181 tgaagccaag ttttcgatgg tgaccaatcc cggcgaaaca caaatggtcg cgtcgcttgg
      241 agttaaattg ggaggccgca cggaaaccgc agaaccattc gaaatgactc gcggaaccct
      301 caaggatgaa gcacaggctg gtgctgggca aatggcagcc tatctgaatg agaagattca
      361 aggtggccag ccggcgacgg cagcaccgaa ggccacggcc cctgcggctg gtggagaaaa
      421 gacctcgggc ggttgtccgt tctcggcgat ggtggcggag atgggattga cctccggcgg
      481 taagccgcag aatggaaacg gcacaagcga atttacgtgg acggcggatg ccaaggaaaa
      541 attggagcgg ttgccgtcat tcgtgaagcc catggttcaa agcagtgtcg aggcctatgc
      601 gcgtaagcac gggtataaga gtattaccct gcaggtcatg gatgattcta agaacgattc
      661 acctaacggt atcgcctgga ccaaggatgc cgaacagcgg atggataaca ttcctgactt
      721 cattcgcccg atggcccgcc gggaaatcga gagaattgcc aaagagcggg gcctggttga
      781 gatcacggcg caagtgatgg atgaagccaa agagaagttc atgaagttca tgtagacgac
      841 gaatgcatgc atgagacgag ttttcggccc atccggcact ccggatgggc cgttcgattc
      901 ctgccggttt ccctctctcg accgaattat tgtctgagac ttctcaggtc ttggtgtgcg
      961 ctgctagcct tgtgttgctt cgtggttctc gcctcgacgg ggtctttttc cattgcagag
     1021 tctgacgacc atggccactc ctccaccgag aatcacgggc atcaccacga atcctcgccg
     1081 tcggcttctc aatgggaagg atcgcgtgaa ggaattgcct attctgaatt caatcatcac
     1141 ttggccggcg tctttcttct gctgatcggg ctgagcgagt tacgccaggc gctgggatgg
     1201 ccggtgctca cctggacgcg ctttttactt ccgggagcgc tggctgtggc cggcctgttt
     1261 ctccttattt ggagcgatca cgatgcctgg ccgatcgggt cgatgacgtt tgccgagacg
     1321 tttttcggga aggatccgga ggtattgcag cataaaacct acggggtcct ggctctagca
     1381 gtgggtacca tcgagtttct gcggcgtctg ggcaggtccg ccaccgcggt gtggaccgcg
     1441 cctcttccga tcttcgccgt cgtgggagga ttcatgttgt tctcacattc ccacggggat
     1501 caccctgccg cggacaaaat tgcgctacat catctcatga tggggaccat ggcgattatt
     1561 gcgggatcat caaaactgat ctccggctgg cgccagagac agctcgcgac ggagcgatca
     1621 tactgggaac tcgtctgggc gatcctcgtt ctcgtcatcg gcgtccaact gctgttttat
     1681 tcggagtagg ccgcaccgtc gccgcggccg cgcagggata gacgctgcgt tgacaccctt
     1741 gtcagtacta tgttagctgt acatttttaa ttcgcgtgca cgtccatagg cccgatgcgc
     1801 aacggggtct gtgagcgcaa agaggagtcg ccatgggtgg ccacagtcat tgggcaacaa
     1861 tcaaacggca taaatcggcg gtcgatgcca aacggggcaa ggtcttcacg cgcatcatcc
     1921 gtgagttgac gattgcggcc cggtccggcg gggaccctga tggtaaccca cgcctgcggt
     1981 tggctattgc caaggccaag gaagccaaca tgcccggcga taccatgaaa aaggccatcc
     2041 agcgcggcac gggcgaattg cccggcgtga catatgaaga gttcacgctc gagggctatg
     2101 gtcccggagg gaccgccgtc ttgatggaaa ttacctcgga caaccggaac cgcaccgtgg
     2161 ctgaaatccg aagcttgcta accaagaacg gtggcaacat ggcggaggcc ggggctgtcg
     2221 cctggcaatt ccataagaaa ggtgtgctgg tcgtggaaaa gggcaaggtc gaggaggacg
     2281 cgcttctcac cattgcactc gaggccggtg ccgaagatgt gaaggtcagc gacaaggcgt
     2341 tcgaggtcct gaccagcccg catgattttg aagccgtcaa aaaagcgctg agcgacgcga
     2401 aaattgagta tacccttgct gaattaaact tcattcccca gaatacaatt gcattagagg
     2461 agaaggccgc ggaacagatg ttgaaactca tggagatcct ggatgagcac gacgatgtgc
     2521 aaaaggtcca tgcgaacttt gacatctccg acgaggtcat ggagaaagtt gccgcggcca
     2581 ccgcgtaatc ccggagtttc ggcactgagt atcgacgcac acacagtcta ttctttaatc
     2641 acttcgcagc gcccgttcct actcatcaga tgatcgcctt actgacaggc cttgtggcct
     2701 tcaaggcgcc ctcgcaagtc acgctcgatg tccatggcgt ggggtacgag gtcttagttc
     2761 ccctcagtac ctattattca ctgcccaatc agcatgagcc tgtcacgctc agcattcata
     2821 cccatgtgcg ggaagatgcc atccagttgt tcgggttttt cactgcctcg gagaaggaag
     2881 cctttctctt gttgaccggc atttccggta tcggaccgaa gctggggctt agcgtattat
     2941 ccacgctctc tgtgcgtgac ctgtacgcag ccattcaggc aggtgatatt gaaaagctgg
     3001 ggactgtacc gggaatcggc aaaaagtcgg cggcaagaat cgccttggag ctgaaggata
     3061 aggtggccaa gttgcatcca tccggagacc tgggtgtggc tggctcaggt cgatccgata
     3121 atccgctcta cgaggatgcc ctatcggcgt tggtgaatct cggttatcgg cagccggacg
     3181 tgaaagaggc gttgaagcga gtggagcaat cgggtgtgct gactcaggga ctggagcagg
     3241 tgattcggga ggccctcaaa gacctggcta aggggtgaca catggtaatg atggagctcg
     3301 cacgaaagaa acaggatcgt cccggtatcg cgatggcctt gggcgtggtg gccgtcttat
     3361 tatgcgcgtc catgacggac cgggtctttg ccgccgacgg catggacgag cccaagagta
     3421 tccgtaaaac ctgcggctcg tgcccggaag gatacgccac caccgggagg acgaatgcgc
     3481 cggaagtctg caaagacggc gatccggtct tggtgcaatg tgtgccctta ggcagcaata
     3541 tgctttcggt ttgtggttct tgccctgaag gctatagtga ggtcggccgc tcgaatgtgc
     3601 cgtcgcgttg cggttccgtc gatggcgggc tggtgtcaca atgtcaatcg cagcagatgg
     3661 ggtcaagtat gcccgatccc agccaaggag gcgtccggtg cccgcctgat tgcggaagca
     3721 cggctgctcc gggacagggc gcgtccccgc cgccgccgaa attccggccc gcacccgaga
     3781 ctagatagtt gcgccccact tctttcgaga ttatgccatg gccgatcgca ccgtaagcaa
     3841 tcaactgacc gacgacgaac gcggactcga ggcggcactg cgcccgcaga gtttgcggga
     3901 gtacgtcggg caaccgcgga tgaaggaatc cttggagatt tgcatcgaag cggccaagcg
     3961 tcgcggcgag gcgttggacc atgcgatttt ctacggtcct ccgggactgg gcaagacgac
     4021 tatcgcgcac attattgccc gggaaatggg atcggcgatc cgctcgacgt ccggactggt
     4081 cttaagtcac gcgggtgatc ttgccgccat tttgaccaat ttgcaggaac gggatgtcct
     4141 cttcatcgat gaaattcacc ggttgccggc atcggtggag gaagcgctct atccggcgat
     4201 ggaggactac cagcttgatc tggtcgtggg gcaaggcgcc tcgacgagga ccgtcaagct
     4261 ggaattgccg cactttaccc tggtcggagc cacaaccagg gctggagcgt taacctcccc
     4321 tctgcgggat cggttcgggt tggtgcatcg gctggaattt tattcgtccc aggagctgac
     4381 ctcaatcgtc acgcgatcag ccggcttgct taatattccc atcgacgagg caggggctgc
     4441 tgagatcgcg aggcgggctc gtggaacgcc acgcattgtc aaccgactga tcaaacgcat
     4501 ccgggactat gcggagatca aagcgggggg ccgcatcact cagcgagtag cccaggatgc
     4561 tctggtatgg ttagcggttg atgaggcggg gctggatgaa atggatcgcc gcgtgctgtt
     4621 gaccgtgatt gagaagtttc acggtggacc cgtcggggtt gattctctgg ctgcggcggt
     4681 tcaagaggac aagggtacgc tggaagatgt ctacgaaccc tatttgatcc aggcgggctt
     4741 tctggagcgc acgggtcgcg gccgacaggc cacgaggttg gcctacgaac atttcaagaa
     4801 acagaaagac ctgttgccgt tttccagtga gaccgattcg atcgcaactc cttgaatccc
     4861 cagacctatc cttgcaatgc ccgcagtggc tcctgtagga tacccacttg tccaacggcg
     4921 cccctttggc ctggactcgt ttcgtagccc gtctttgtga aaggcgcgtg ttgcgagaag
     4981 ggaatccccc cgtcatgtca gatgacttac aagtccatcg cgaagaaatc gaccgggtcg
     5041 acgatcagat tttgcgtctc ttgaatgagc gttcgaaatc ggtcatcgag atcggccggc
     5101 tgaaaaagct caaagatgcc gacgcgaatt tgcacacgca ggcacgcgaa tcggcgattt
     5161 tcgatcggct cagcaagcaa aataccggcc cgtttcccac cgaggccatt cgtgcagtgt
     5221 accgggaaat catgtcggcc tcgttatccc tcgaggggcc tcaaaaggtt gcctaccttg
     5281 ggccgagggc cacgtttacc catatggcct gcatgcaaaa gttcggcgcc tccgcgcagt
     5341 atattccggt caacagtatt aaggacgtct tcagcgaagt ggagcgcgga cgcgcccatt
     5401 tcggcgtcgt gccgatcgag aacacgacgg aaggcgtggt gaatcacacc ctcgacatgt
     5461 tcgtcgattc caacctgctg atctacggcg aagtcctgca ggaagtcgcg catcatctga
     5521 tgtcaaaaag cggactggcg ggagatatca aacgtatcta ttcgcacccc catgccatcg
     5581 cgcaatgccg aaattggctg gagacgaact tgccgcacgt gccggtgtcg gaagtggcca
     5641 gtaccgcgcg ggccgcagaa atgtcggttg atgatccttc ggctgccgcg attgcgtcgg
     5701 aattggcatc ccaactttat ggcttaaagg tcgtcacggc gaggatcgaa gacaatatca
     5761 ataacttcac gcggtttttg gtgttgtcac aaaaggcgcc ggagcgaacg ggccgggaca
     5821 aaacatcgct gatgttatcg gtcaaggaca aagtcggcgc cctgtacgat ttattgcgac
     5881 cctttgcctc ccatggcctg aacatgacta agatcgaatc gcgtccctcg cgtcgcaaag
     5941 cctgggaata cattttcttt gtggatatcg aagggcatat cgacgaggaa cgggtcaaaa
     6001 aagcggcaga agaggtcaag agccgctgcc tgttcatgaa gattctcggg tcgtatcctg
     6061 cctattccta gagggccgag ttacaccatg ccattgaagg tgcaccctga catcgtctcc
     6121 ctcgtgccgt acgtgccagg aaaacccatc gaagaactac agcgagagat gggattgccg
     6181 cgcgtggtca agctggcgtc aaacgaaaat ccgatcggcc cgtcgcctaa ggccatggca
     6241 gtgctggccg agacggttcc gaccctgcac cggtatccag acggcggagc gttccgcctg
     6301 cgtggggcct tagccgagcg ctggaaagtg gcacccgacc aggtcattct cggaaacggc
     6361 tccgacgaaa ttctcggcct cctcgcacga accttcctgt cgccgggaga cgaggccgtg
     6421 atggccgagc ataccttcgt gatttataaa atggaggtcc aggcggccca cggtgtggcg
     6481 gtggaggtgc cgcagaaaaa ctggcatcac gatttgccgg ccatggccgc tgccattacc
     6541 gagaagacgc gattgctgtt tgtctgcaat cccaacaatc cgaccggcac catggcgacc
     6601 aaggcggaag tagctgccct catggagcgg gtgcccgacc atgtcgtagt cgtgttcgat
     6661 gaggcctatt acgaatacgt gcggcattcg gaatttccgg aatcactcgc ctacgtcaag
     6721 gccggacgac ccgtcatcgt cttgcggacc ttttccaaga tttatggatt agccggttta
     6781 cgcatcgggt atggcatcac cacccctgaa atcaccaatt acctgaatcg aatccgtcca
     6841 cccttcaatg ccaacagtat ggcgcagcgc gctgccttgg cggcgctgga cgacgacgct
     6901 catgtggcgg caagccgctc actcaatcac gtggaaatgg acaaggtgcg tgccggtctg
     6961 cgccggctgg ggttcgaggc cttgcccagt gaaacgaatt ttctctattt cgatgtcggg
     7021 agaaacggcc gcgcagtctt tgacgcgttg ttggccaagg gcatcattgt gcgtcacatc
     7081 gagggccgca tgttgagggt gaccattggc ctcccggatg aaaatcaatt gttcctgagc
     7141 gcgctggaag acgtcacacg cgcgtcgcaa taaggaaagc gagacgagac catgattatt
     7201 gtcttgaagc ctgatgcttc ggagcgggaa gtcgatcaca tcaccgaccg gcttcgggaa
     7261 ctggggctga agtcccacat ctcggcagga caggaacgca cgattatcgg ggtgatcgga
     7321 gacgaccgga tcttgcacaa tcagccgttg accgccttgc ccggtgtgga aagcgtgttg
     7381 ccgattctgg ctccatggaa actcgtcagc cgggagttta agaaagaaaa caccatcatc
     7441 gatgtgaacg gggtcaagat cggggataag aaaattacga tcatggccgg tccctgcgcg
     7501 gtcgagcgct tggaattaac cgtgggtatt gcgcacgaag tcaaatccgc cggcgcaacg
     7561 gtgttgcgag gcggggcgta taagccgcgt acctcaccct attccttcca gggattggga
     7621 cgcgaggggt tggattattt ggtggaggcg aagaagcaga ccggtctgcc ggtcgtcagc
     7681 gagatcctcg acactcgcga tatcgagctc tttctggaaa aagccgacat cattcaaatc
     7741 ggcgcccgca acatgcagaa cttcgagttg ctcaaggaag tgggcgcgta cgataagccg
     7801 gttctgttga agcgcgggct ctcggccacc atcaaggagt ttctgctttc cgcggagtac
     7861 gtcatgtccc gcggcaatcg gaatgtcatg ttatgcgagc ggggcatccg cacctttgaa
     7921 acgcaatatc ggaacacgct ggaccttgcg gccatcccga cgctcaaaga tctttcccat
     7981 ctcccggtca tcgttgatcc cagccatgcc accggcaaat gggatttggt agcgccgatg
     8041 tccaaagccg cgatcgctgc cggagccgat gggctattga tcgaagtcca ttccaatcct
     8101 gaatgcgcgc tgtgtgacgg agaggaatcg atccggccgt cgaaatttaa agaactgatg
     8161 ggagacttgc gcaagatcgc tgctgccgtc gatcgcacat tgtaagggat cgcggagcga
     8221 cgttgaccag ggaatgtccc aaggatcagc caattacctc atgacgacac cacatttcaa
     8281 acaagcggcc attatcggag tcggactcat cggcggctcc ctggggatga tcctgcgccg
     8341 tcagaagctg gccgattcag ttgtcggcat cggtcgccgc gtggagaatc tgaaaacggc
     8401 ggttgaggtc ggcgccatcg atcgatacgt gtctgatccg cgcgaaggcg ttgaacaggc
     8461 agattttgtc ctgctggcca caccggtgga tacttatgag cggcacttgc aggaatgggc
     8521 cggctgtctc aagcccgata ccatcgtgag cgatgtcggc agtgtgaagg gggaattggt
     8581 cacgagggcc gaggccctga tgccgtcagg ggttaaattt gtcggcgcac atcctattgc
     8641 cggcaaagaa aagaccgggg tggcagccgg ctccgagacg ctcttttccg gtgcacgctg
     8701 cattctgacg ccaaccaccc acacagatcc cgaggccctg aaggttgttc gtaaaatctg
     8761 ggaactcgcg ggttcgattg tgctggagat ggatcccttt ctccacgata aaattctcgg
     8821 tgctgtcagc caccttccgc atgtagcggc ctttgcattg atgacggcct tagccgatgt
     8881 ccgcgaccat ggcgtgccgg aactggacct ggctggtcat tccggcggcg gcttgcgtga
     8941 caccacccgg atcgccgcca gttcgccgga aatgtggaga gatattttcc tctggaatcg
     9001 ggacaacgtc gtgtcgttga tcgagaccta tgaacgccat ctcggtgagc tcaaacggct
     9061 gattgccgcc ggcgatgccg ctggaattga aaagcaattg gacaaggcca agtacgaacg
     9121 tgaacaactc atcccccgga caccggggaa ggcctaagct gcgatggctt cgttaacgat
     9181 tactccaggt cgtccgttga agggcaccat cacggtgccg ggcgataagt ccgtcacgca
     9241 tcgggccatc atcctcacgg ccttggccga aggatcgagc accgtgaagg agtactgtcg
     9301 tggtgaagac tgtctgaaca ctatgcgtgc cttccagtcg ctgggggtac ggatcgagga
     9361 gacgccggaa tcgctgacgg tctacggaaa aggcatgtgg ggactgacgg aaccattcgg
     9421 tcccatcgat tgcgggaatt ccggaaccgg tattcggctg atggccggat tgctggcggg
     9481 gcaggatttt tttacggtcc tcacgggaga tgaatcaatc aggcgccgtc ccatggggcg
     9541 ggtggtcaag ccgttgcggg cgatgggtgc gactattgcc gggcggaagg gcggggagct
     9601 tgcgcccttg gcgatcacgg ggaccaggct caagggtctg tcctatgttt cgccggttgc
     9661 gagcgctcag atcaaatcgt cactgctttt cgcggccctc tatgcggacg gcctcacaac
     9721 catttccgag ccccggctct cgcgagatca caccgaacgt atgttcgcgt atttcggcat
     9781 tccgttcgag cgggacggat gcacggtccg gatcgaaggg cgtccttcgg tccgttggag
     9841 cggtaagccg gtgctggtcc ctggcgacct gtcggccgcg gcattttttc tggtcggggc
     9901 ctccattgtt ccaggttccg atgtcacggt tcgcaacgtg ggcatgaatc cgacgcgaac
     9961 cggactgatc gatatcctgc gtgagatggg cgcgcatatc gaggtgctga atccgcgaga
    10021 ggaggcaggg gagccggtag cggatttgcg ggtgctctcc agaccgcttc acggcgtacg
    10081 catcggaccc gatcagattc ctcagaccat tgatgaattt cccatcctct gtgtggcggc
    10141 agccgtggcg gaaggtgaaa ccgtggtcac cggcgctgaa gagctgcgcg tgaaggaaag
    10201 cgaccggatt gcgaccatgg cgaaagagtt gcgggccatg ggagcccgta tcgaagagcg
    10261 gccagacggt atggtcattc aggggctcgg acacaacggc gccaatggtg cgctggccgg
    10321 cgccatctgt gaaagtcatg gcgatcatcg tgtggcgatg tctgtcgcga tcggcgggtt
    10381 gactgcggcc caggcaacac agattcagaa tacggcctgt atcgaaacat cgtttccgaa
    10441 ttttgaatgt aagctcttgg aactgttgac tgattctggg aaacgtctat agtgcgagga
    10501 tcgcgtgggt gaaattgggg ttgccagccg cggcaagcgt ggcttgatca tcgccatcga
    10561 tggcccggcc ggcgtgggga agagcacggt tgcgcgactg ttggccttgc ggctcggata
    10621 tctctatctg gataccggtg ccttgtatcg ggccattgcg tggaaagtcc acaatgcggg
    10681 actcagtcct gacgatcacc ttgccattgc tgcgttgctt cccaagacaa ccctgcagat
    10741 ggcctgtggc cctgaacaat cccatgtgct cctgaacggg cgcgatatta caggtgagtt
    10801 gcgaactccg gcagtgagcg cgctcgcgtc aatggtctca gccattcccg ccgttcgcga
    10861 atggctgttg cccgtgcaac ggcagatcgg cgctgaaggg tgtgtggtgg cggaagggcg
    10921 tgacatcgga accagggtat ttccggaagc cgacgtaaaa tttttcctcg aagccgatgc
    10981 agaagttcga gctacgcggc ggcatcgcga actggtggcg gcggggcact cagtgcaatt
    11041 tgatcagacc aagcgcgacc tgaccgggcg cgacgatcgt gaccgtaccc gcgcggtggc
    11101 gcccttgatt cccgcatccg atgcggagcg gctcgatacg tcgagcctgc cggtggagga
    11161 ggtcgtctcg cacatgatgg ctgtagttgc ggcgcgattg tgagctccgc cctgtatggc
    11221 ctcttgtggg tcctctctcg catcatcggc tggctctgtt tccggtatcg caccatcggt
    11281 caggtgccgc ggaagggcgg ggtgttgatc gcctcgaacc acgccagtta cttggatatt
    11341 cctttgctgg gctgcggggt tccccggcgg gtctggtata tggggcggca tgatctcttt
    11401 ccggtcccgc tgctcaatgg cctgctgcaa gcgttgggat ggattccgtt acgcatcggc
    11461 cgccttgatc gggacgcctt cagtaaagcg gtcgccttga tcaaagagga caaggcggtg
    11521 gctattttcc cggagggagg gcggacgatg accggagcct tgaaaccggg gaagcccggg
    11581 atcggggtca tcgtgtcgca gacgggatgc cgcgttgtgc cggcacatat cggcgggacg
    11641 ttcgaagtgc tgcctcccgg ggccaagtgg ccgagatttc gccgcgtgac ggtgacctat
    11701 ggcgaaccgt tggatttctc ggcagacgcc gcgcagttgg aaggcaaagt gttttatcaa
    11761 catgtcagtc gtacggtgat ggccaagatt gcagagctgg gacaggtgcc gaaccccgga
    11821 gaggggcagg cccacgccgg gctcactcac gatcgagaag ccacaccgac agccaagtct
    11881 tgcaacgctg agtaagactc ggattttttc actatcagca cccggcatag gccggaggac
    11941 ggaccgttta tgagtactgc cacaccacaa agcgaaccca agctcgacca cgatgcctta
    12001 gcggcgatgt atgaggagac cttccggaat tttgaggaag gtaccatcac ggagggcatg
    12061 gtggtcgcga tcggcaaaga caaagtggtc gtcgatatcg gctacaagtc tgaggggatg
    12121 attcccgccg accagttctc acaggaagaa ttggggcaat tgaaagtggg cgatcgcctt
    12181 caggtgtacc tcgaagaatg tgaagacgct gatggcaacc tggtgctctc caaagaaaaa
    12241 gccgacaaga tgaaaatctg ggaggaattg gagaagctgc acaaggaaga aaaaagcatc
    12301 gaaggtaaga ttatttcgcg catcaagggc ggcatgatgg tcgatatcgg cgtcaaggcg
    12361 ttcttgcccg gttcacagat cgatctgcat cccgtccgcg acctggatgg gttggtcggc
    12421 aagaccttcc ccctgaagat catcaagatc aaccatcgcc gtggcaatgt ggtggtttcc
    12481 cgacgggtct tgctggagga aacccgcgat cgacgccgtc agacgaccct gtccacgctg
    12541 aaggaaggcc agttgattca aggcacggtc aagaacatca ccgattacgg tgccttcatc
    12601 gatctgggtg gaatcgacgg gttgttgcac atcaccgata tgtcctgggg tcgtgtcgga
    12661 catccttccg aattgttcca ggtcagcgac aaagtcgaag tcaccgtgct caagtacgat
    12721 cgggaaaccg gccgtatttc gctcggcctc aagcagaagt cggcggatcc ctggaccggc
    12781 gtggccgcga agtacccggt gggaacgcgc gtgcgcgggc gggtcgtgag cctgaccgat
    12841 tacggcgcgt tcgtcgaact ggagcccggc gtggaaggat tggtccacgt gtccgagatg
    12901 tcctggacgc acgaagtgcg acatccctcg cgtgtggtgt cggtgggtga tcaggtcgaa
    12961 gcggccgtct tgaatatcga tccgggaagc cgcaagattt ccttgggcat gaagcagacg
    13021 gccccgaatc cctgggacat gatcgaagcc aaatatccag ccggcacgcg gatcgaaggc
    13081 aaggtgaaga gcctgaccga tttcggcgcc ttcgttggct tggaagaagg catcgacggg
    13141 ctgatccaca tttccgacat gtcctggacg aagcacatca agcatccgtc ggagctcttc
    13201 aagaagggcc agaaggtgga tgccgtcgtg attcgcatcg ataaggaaaa ggaacgtctc
    13261 tcgctggggt acaagcaatt gtcccgcgat ccgtgggaag agcagattcc caacaaatat
    13321 cgggtcggcg atagcatcac cggcaaggtc agcaagatcg ctgatttcgg cctctttatc
    13381 gaattggatg gggacgtgga aggcttgatc cacatcagcg aagtgggtct ggacgccaac
    13441 gtgcgcatgg aagaaaagtt caagccgagt gatgatgtca cggcgaagat catcaaggtt
    13501 gaccgcgatg aacggaaaat cgcgctcagc cttcgcgatc atcagctgga ttccgaccgt
    13561 cgccaggtgg atgagttcca tgcctcgcag ggcggaatcg atcaaaccct gggtcgcgcg
    13621 gccaagcaaa gccggaagcg taaccagagc gattccgaag cctaaacgcc gggagactga
    13681 atgggcccac aagcagacac ggcggggaaa ccacaacgga gcctactgcg ccgtatcctt
    13741 tgggccatcg tgattgtggg aggagcgttg attctgttga acgcgctcct ccccgacctc
    13801 gatttctcca cccaggatcg tgtggccctc attcgcatcg agggggtgat cctggatgcg
    13861 caagcgacca tcagcgagtt aaagcaatac agcgagaatc cgctcgtgaa ggcgatcgta
    13921 ttacggatcg acagccccgg cggaggtgtc gtaccgtctc aggaaattca cgacgccgtg
    13981 aagcgggtga aaaacaaaag taacaaggcc gtcatcgcct ccatgggaac ggttgccgcg
    14041 tcaggcggat attacattgc ggcagccacc gaccggatca ttgccaatcc cggcagcctg
    14101 acgggaagta tcggcgtcat tatggaaatg gcgaacttcg aaggcctgat gaaaaaagtc
    14161 ggggtcgaag gcgtcgtcat caagagcggg cgttttaaag atgtcggatc gcctttacgc
    14221 aaaatgagcg atgaagagcg taagctgctg cagtccgtga tggacgacgt gcatcagcaa
    14281 tttattcagg ccgtggcaga cggacggtcg ctggaagtgt ccgacgtaga gcccttggcc
    14341 gatggccgga tttataccgg gcggcaggcg aaagaggcgc ggctgatcga cgaattgggc
    14401 gatctcgacg acgccatcca tattgcggcg gacatcgccg gcatggaagg cgagccgaag
    14461 gtcgtcgagc cacgcaaacg attttcattc cgggagatca tcgaatcccg gtggtcgtcg
    14521 gtgtttccca agctggagtt gaataccggc gtgaaactga aatacctgat ggcgttctga
    14581 gcgcctcgaa gcatgatcgg catggcgacg cagcaattca cctgtaggta agccccaccc
    14641 acgaggagag ggacgatgac caaagcgcag attatcgagc gggtatcgga gcaggtcacc
    14701 acgttgacga agcggcaggc ggaaattgtg gtcaatacga ttttcgattg cattcgggat
    14761 tccctgcgta gcggcaacaa gacggagatt cgagggttcg gcagcttccg tcttcgtgcc
    14821 cggcgcatga aagaagggcg caacccgaag accggcgcga cagttgcggt gccggctaaa
    14881 cgtgtgccat tcttcaaggc aggcaaggaa ttgaaggaat tactcaataa gtaacaggag
    14941 tgatgccgtg gcagagtcgt ccaatataac agcccctgag gtgcggtgga gtgacggtgc
    15001 gcttaaacgc atggagcgcg cacccatgtt tttgcgggga atggtccgcc gcctggccga
    15061 gaagaaggcg cgtgagttgg gctatgccga aatcaccgaa gagatcctcg agcagttcaa
    15121 agggcagatg atggggacca tgggtgggga aggcggcatg gcagaggcgg ccgatcaaat
    15181 ggccaaaggc cagctgccct ggaccgccgc cgccaaggag cgcctcgctg cggtccccga
    15241 gtttatgcga gggatgatca agcagatcgc cgatgagatt gctcgcaaag gcggccacat
    15301 ggaggtcaat gtcgacctgt tcgaaaaggt cgaagccttg ggagagatcc acgagcgcga
    15361 aacgactccg ttggaatgga ccgaaggcgc attagcactc ctgcagcaga agatcaaaga
    15421 gtctccgccg atcgccatgg acttcgtgag tgacatgatc cggcatgata ctgaggagat
    15481 ggcgcgggag aaggggttga ccagaatcga cgagcagacc gccgtgcagt tgtgggaagc
    15541 gcctcaggag cgcgtggcct ggagcgacga agcctggaag cgtctgcaga cctcgcccga
    15601 ttttgtgcgc agcggcattc gtaaagcggc cgaacgcagg gcccgcaagt tgggtttgaa
    15661 agaagtcgac tcagagcatc tcacgacgtt tcgcaaccag gccatgatga aagccgtgaa
    15721 gcgaatccgg tccttcggat ataacgagct gacgtttgat gcctttgaca ccgcacttca
    15781 aaagaccaag cggttgcaag gcaacgatca agcggaaaag cggctgcagg aaattcgttc
    15841 ccacttcagt gatcccgaag tgaaaaagcc ggaaggcggc actttggggg ccgacctcat
    15901 gggacgattc agaaaattcc tcaagggcga agggtctctg taagaatcga cggcgccgcc
    15961 atgatgatgc ctggcggcgc tgttctcatc tctttctcca cgtcgctctc acttccactg
    16021 ttgtgcttcc atcttgtgtc tggggtgccc ccacgcacat ggcacggcgt tgcacatctc
    16081 ctgcatttct tttgagctct cggcgatggc aagatctgac agatcgtctg aagcggctga
    16141 aattgaaaca gccttctcag ggatgatcaa actggtcgac gctggtgcga gagcagacgt
    16201 gaaaagtgat atcacccaca agccaagggc ctgtgggtga tatcggacat tgagggaagt
    16261 atcgagactg tcgattagtg agccgcgatg ccggctgctt ggggccagaa tttatgccgg
    16321 atctggggca gcggttcgtt atcgaagttc ggaatgtcgt agaggcaacg gtcaacggtc
    16381 gaaacttcgt cttccgtcaa ttccagtgcg acttgtttct tccagaattg atcgagcgcc
    16441 agatagtcgt tggacgtcgg cttctcggcc aacagggact gcatccgctt gaacccttct
    16501 gtatcgactc ccggcacacg gccgttattg cgatcaatac accatttcag gacttctgcg
    16561 acgccataca tcgacaacgg aattgtgact ttcttgctca tgggcacctc ctccacactt
    16621 gggccgcatt gtagcctaag gctttccaag ttttcaaagg ggcgaaaggc ctagacgtcc
    16681 gagtccattc ggggggagca caacgaccaa gccccacacc gcaaccctga cgcggggact
    16741 tcgttgcgaa gcagaagggg gctctgtata ctccgctcag cgcggcagga ttgggagcgg
    16801 cgaagcatgg acttggccgg aatgtcatgc ttttttcatc gggatggcga tgaccatcaa
    16861 tacctatgcc aacaagaggc gggcgatcgg tcgagtgggg ttggcactca tagccggctt
    16921 gccgctcctc ttcgcctgct ccaaaacgga tccgtacgtc ccgcccgatc cattttatta
    16981 ctttgaaagc tatgccgtcg gcaaaaaccc gacgaccgtg acgacggccg acttcaatcg
    17041 ggatcaaatc accgacctca tcacgaccaa catctcgagc aattcgattt cccttctgtt
    17101 cggcaacggc gacggaacct ttcgggacca ggttcaggtc aaggtatgtc aggagccacg
    17161 gtccctcgcg ctcaacgact tcaaccatga tgaccagctc gacgtcgcgc tggcttgttc
    17221 aggaggggat cagatctcca tcctgttcgg gcgaggcaac ggaaagtttg atgaaggtcc
    17281 ccagtacccg gtccatcgcg ccccggtgtc catctccagc caggacgtca acggcgatgg
    17341 gcatccggat ttagcggtgg ctttacgcaa cgacaaggtg aagatttttc tcgggagcgc
    17401 ccgtggcgaa ttccggcccg gcgtgcaata cgaatatggc gatactccca cctccgtggc
    17461 gctgaaagat ctcaatcaag atggccagcc ggatttggtc gtcacgaacg gtgggccgat
    17521 gttgagcgcc gtctctgtat ggattggcaa tggcgatggg acgttccgcg atcccaaaga
    17581 ctacaaaacc ggtcggcggc cgttgggggt cagtttcgct gactttaaca acgaccacct
    17641 acctgatctg ctggtcatca acggggaggg ggatagtttt accaccttcc tcggcaacgg
    17701 gaatgggacc tttcagccgg ggaaggattc cggcgcggat gccagcccca atttcggcgt
    17761 ggcccatgac ttcgatggcg accatatcga cgatgtagcg atcgtcaatt tgcagtccac
    17821 ggacctgtct attttgttcg gccgtggcga cgggacattc cattacccgc cgcgtaacta
    17881 ccgcacgaaa aacgggccgt ttgccgtggc cagttatcag gtctctgccg acaacggaga
    17941 ggagccgggg cttgtgacgg ccgataacgg ggcggccagt gtttcggtgt ttctgcatca
    18001 cggccggaaa gggcagatcc cgccaaacag taaaccggaa tagcggccga ttcagatttg
    18061 agccgcgaca accgaagaag agacaggcag tttgcttgac acctcgatag ggctcggata
    18121 gagttcgaaa aagtcgctgg acacaggtcc tgcgccattt tcagcgatca gaccgcgggg
    18181 gctcgtgcga tcgtttttgt ggtggtttag catgggctgc ctgacgacgc tggcagtctt
    18241 gatggtccaa ggcggggtac gcgatctggt gtcggggacc aacccgatgg gaagttccgg
    18301 cgcgatcctg tccttcagta ccacgatctt cgggggtggg ttgttggccg gctgcctcgc
    18361 ccttatcctc aa
//
LOCUS       DAOQEW010000065        18032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_806_length_18032_cov_8.087946, whole genome shotgun sequence.
ACCESSION   DAOQEW010000065 DAOQEW010000000
VERSION     DAOQEW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 18032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18032
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_806_length_18032_cov_8.087946"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /locus_tag="PLS35_08955"
     CDS             <1..2007
                     /locus_tag="PLS35_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNI18831.1"
                     /translation="EAGLTEALRLKESRDRYREAERLAQLGRVTEAMTGYMRAVELDP
                     TYKDALEGVARLSAEQHAADRDDRQKQPVTLQFRNAGLKEVVEALGKAAHVNFVFDKD
                     VRNDPITVSLEEKPFDEALSLVLNSNSLFAQKAGPTLFIISPNTKQKQEQYQDLMIRT
                     FYLSSAKAKDMVALLKSMLDVKHIHGNEALNTIVIRDQPEKVELAEKIIQANDREDSE
                     VLFDVEVLEVNRTVDQQYGLNYPKQIGAGLVPPGFTGTIAGDLAQQFTYRNLASLGQD
                     SYLFKVPTNVQLDFFKQITDAKTLAAPKVRVLNNKKAEVNIGDKQPILLSTTNVLPGQ
                     AATGAVPTTSTVTSIEFRDTGVKLTVEPNIHLANELSLKMKIEVIRLGDTVLLQQSPP
                     ITQFKFGNRSAETMLNVRDGETIILGGLLQEEDRRTKVTIPWIGDIPYLGNLLSSFKT
                     QRVTTEVILTITPHIVNSLRIPGPQGQAFWSGTESVYSTSPLFAMQPKPISARVAVSA
                     AAGSAIEKSGSKKSKMNAASSDAVSPRLQLSMQPADATVQMEKEIRVDVVATQAQGFD
                     TETFTLEFDPKILEFRDATLGEVLGTEAGKAPVAATSSSTEGIVELRLHRSSTATKDE
                     GRLLRLTFLAKSPGVSPVRLQMAKHPGQDLPEGTGEASGVVRVR"
     gene            2004..2510
                     /locus_tag="PLS35_08960"
     CDS             2004..2510
                     /locus_tag="PLS35_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI18832.1"
                     /translation="MKQSGVTLLELLVTLTILTILASVALPFTKVSTKRTKEIELRQN
                     LRVIRAAIDAFRLEWARDGDTLIGPACVKNRLSCKDVTGPYGYPKSLDALLGVKLTGE
                     QATVRGTTIRRYLRSIPMDPMTGAADWRLRCYRDPASVKDWCGEDIYDVTTQSQELAL
                     DGTKYREW"
     gene            2485..2892
                     /locus_tag="PLS35_08965"
     CDS             2485..2892
                     /locus_tag="PLS35_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI18833.1"
                     /translation="MVRNTESGRRAWVSSSAHGFTIIELMIVVTIVGILATLAVPSYH
                     AAIIKAKEGALRQDLFSLRDVIDQHRADKGKYPESIQALVSAGYLRRVPTDPLTGATT
                     SWQEMVDEAEGGMVDVFSGSELVGTNGVPYNQW"
     gene            2906..3736
                     /locus_tag="PLS35_08970"
     CDS             2906..3736
                     /locus_tag="PLS35_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNI18834.1"
                     /translation="MIQPHVPARRKGVGLRLGCWLAALLLCESWGGCAKVTPNHEPAP
                     SDLQVAADTLKVAVREAQRTAAELRTELEEQRKELADAQVARAQLQGMLRETERRLTE
                     ARQIIELQREELTSARDERERLAQAVHPFTGHVKASSAPAPSRSKRSPAAQGAAIPGS
                     VDTREMRDDNSLVEQEPAAPQPLPLHREATDSSTAQASAVHVAPLPKGKAVGRTIVVQ
                     GGDTLWGLARRHKVEVAVLRALNGLSGDRILSGRKLRLPESEAQHAAVQPAAVDVVVQ
                     "
     gene            3779..4993
                     /locus_tag="PLS35_08975"
     CDS             3779..4993
                     /locus_tag="PLS35_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNI18835.1"
                     /translation="MAVFSYRAARADGTTFEGHIEGDDEHVVRAKLEADGVLVFRLSR
                     SGTGFGVPGLSASRWGKLPLQDFLVFNQELLALIKAGLPVLRVWDLLIDRTQRAPFRE
                     ALKAVRQDIRGGASASEALAKHSTYFSDLYLATIRAGEQSGNLAEVLQRYIAYLKLMI
                     SLRQKVTKALAYPGFLVVVGVAVVGFLLSYVMPTFISVYGESSANLPTPTRLLISVIH
                     MGEAQLIPVAIIVIVATVMGRAWYQTSAGRLSVDRNLLRLPLLGTILVEHHTIQLTRT
                     LATVLAGGTPIVEALEIARGAVSNRFVSRGLVSAVNEIREGSTLAAAIERPKILPKLA
                     IEMLSVGEETGSLEPMLRDVAEFYEGDLDVRLSQLTTWIEPVLLLVMGLLVGGIVIIM
                     YLPIFQMAGTIQ"
     gene            5057..6790
                     /locus_tag="PLS35_08980"
     CDS             5057..6790
                     /locus_tag="PLS35_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNI18836.1"
                     /translation="MAMQRHLTRPSLADVLVREGILPRRTVDQVVARLGGSTTALGPT
                     LVEDGTISEMQLAQALAAQYGLPYDALTGFRVDSEFYHTISVKLMRRHPFVPLKDEAG
                     ILTIAISDPQNLLGLDELEILLNRPLHYVVSSKNAIQAALERSEGSSQALRELEAEYR
                     SVLVKEDERGEEVLTVDHFGEDQSPVVKLLDTIMLSAMQRRASDIHIEATDRATTVKF
                     RVDGILVSAMDPLDVKLHPPLVSRLKVMSDLDIAERRVPQDGSFRMRLDRKTVDFRVS
                     ILPSVFGESVVIRILDREAITTGVSTLRLDRLGFNPEDLKRFRRAITRPYGMVLVTGP
                     TGSGKTTTLYAAISEMNIQEDKLITIEDPVEYQLPGVVQIPVNEKKGLTFARGLRSIL
                     RHDPDKIMVGEIRDAETAQIAIQSALTGHLVLTTVHANNVFDVIGRFASMGIDSYNFL
                     AALTCVLAQRLIRVICPDCRHQVTLDQALAEESGLDYDEFKTSPFYEGKGCSECHDTG
                     YRGRKCITEFLDLTDEIKEMILADRALSEIRYRAVTDGMITLRQSAVKKVLAGETTLR
                     EINRVTFSEER"
     gene            6794..7801
                     /locus_tag="PLS35_08985"
     CDS             6794..7801
                     /locus_tag="PLS35_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18837.1"
                     /translation="MWDWITSRPQYCLKIGAHDLAWAEVHRNWRGRPRYQCVVSALPD
                     GILRLSPLEQNVVSPHELESHIRAIAGSATTQAPGRSSTPGVPRAATVILPDLAVRLA
                     VVRLQDLPWSSDERDALVRWRLGQEQLLSLSGAKVFSQVLSEPGVSGDGPCTVLAVVV
                     QEAVLSQYEAVCEAAGLIPQEVDVASLRLFNLWSHGAWRTAGSTADFLWVNATEGGVT
                     AFIFHKGVLVFLRSKLQGGVGSSGIWPAGSEQLGINRIVQECADSIYACQQQTPELAI
                     SQTILIADDALGPGLRQQLEKGLGVPVQELDWERVQQFGGGPIAGPRTSAAMPAIAGV
                     V"
     gene            7805..8428
                     /locus_tag="PLS35_08990"
     CDS             7805..8428
                     /locus_tag="PLS35_08990"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNI18838.1"
                     /translation="MMLDNLASVLRDVASRIGQPSARSGVFTINLSSRYRWYLAPARL
                     LIMVLAGVIGVAILWDISQSWLVWQDVQARESALLQVQERDNELLKEAQQEGLDLSEA
                     ALQLLPKEIEFANHLIEKRSFSWTRFLTELERAIPQRIAVNSIRLDPANATIMLTGSA
                     RALEDVTTLTVMFQDHPQFKDPVLGQHRVMGNGLVEFDLTLRYRNRS"
     gene            8431..9000
                     /gene="pilO"
                     /locus_tag="PLS35_08995"
     CDS             8431..9000
                     /gene="pilO"
                     /locus_tag="PLS35_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNI18839.1"
                     /translation="MFPGLDRLQLTGRQPYAPILPWVAFVVVLAIVSYGIHALVLGAA
                     EEEQVRLEAESVKARQQLNRHREAKKAKADLQRVWAVLPLFRDFAPLALGVTEEAKRD
                     QVTLPALSYKTEKTSVPDATKAVLQGTVTGRYEDLRRFLYNLESAQELLFIEDLNLVR
                     SGNIQDQQLTFNIRISTYLRGEHLQSPTP"
     gene            9006..9719
                     /locus_tag="PLS35_09000"
     CDS             9006..9719
                     /locus_tag="PLS35_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18840.1"
                     /translation="MNRVSKNQGMLLLTLVVFWIGLLIWQFDHSNEQARVPLTHVSGQ
                     RATSKTPQAASSGNLQVHLERLAAIKGQREATFSTPRNIFASLLPQVEPVAVPPPAPR
                     RGRSRGPAKAAPPPVLEPLGEPQEQTAAEEEQEPVVEGPKGPTPEEIERLRIATELNQ
                     YRYVGFMAVGGGSQKKKNMAVVVKNDEMHVVQVGEVIGGEVLVKDVSLKEITLQDVGS
                     KITQVIPVTDDPGAGGQPN"
     gene            9781..10335
                     /locus_tag="PLS35_09005"
     CDS             9781..10335
                     /locus_tag="PLS35_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18841.1"
                     /translation="MFSIVLIGISVSAAARQWTVMVQREKEADLMAKGIEIQNALALY
                     SASMKIGRIMTTEIYPQSLAELTRTPKPFLRRVYQDPISGGDWEYMRAPTGGIMGVRS
                     KSKVKPIKERDFPLAVRHFEGRKTYHDWMFQHPNPSSQSMLMPPMMGLAPGTMPTHPG
                     AQGAVPGGGTPQGAPLMPGAPRNP"
     gene            10458..11771
                     /locus_tag="PLS35_09010"
     CDS             10458..11771
                     /locus_tag="PLS35_09010"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNI18842.1"
                     /translation="MEQDLTESPAPELEAPVEVLSPEDYVMGLVKSAKKAAGRLSTLS
                     AAVKNEALEAMAEGLVAHEAELLAANEKDLEQFDATPERKALGDRLRLTAERIRDMAA
                     GLRDVARLPDPLGEMPKMWTRPNGMQVGRMRVPIGVIGIIYEARPNVTADSAALCLKS
                     GNVCLLRGGSEALHSNTAIAKVLSESAEKAGVPAGAITFVDRPEREIVQLLLKQDQYI
                     DLIIPRGGDSLMKTIAEHATIPVLKHDKGVCHTYVDADADPKVAEHICMNAKVQRPST
                     CNAMETLLVHHSIARTWLPHLIKQLTAAKVEVRGCAKTCQLCPEVKPAVEDDFGHEFL
                     DLILAVKVVKHMDEALEHIATFGSRHTEAIVTKDYSRAMRFLREVDAGAVLVNASTRL
                     NDGYQFGLGAEIGISTSRLHARGPMGLEELTCSKFVVLGSGQIRE"
     gene            11772..13550
                     /locus_tag="PLS35_09015"
     CDS             11772..13550
                     /locus_tag="PLS35_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18843.1"
                     /translation="MPVDVSLQEILSRPGTLAFPSNLADAPRLPASSASCLRLPTGHL
                     VVYGSHGRRILATDPDGYPLHECEWESVNGRLRLVRSRVHLDWGAWVGLIPGGLVNTM
                     TLDLSRKPGWQRLRADDLRSMAAQAMHVPLDEVRFFYSDQDMTIDAKGRATIRHRKDA
                     IYHLPDGTFDTEQFMACMGAMHWEAIDFLPVVELFLSLLPGTGSALFELIRGLYDDQN
                     RGQVAPRPLRYRGIPTYPSEAAYRLFSNFFSPHLTDGSDPFPVFMDTPRSHLVTWTPV
                     PDPPRRHVDLSQKLCVTIQGRRMLKATRWDDPAGLSYQPFDQRGLAPCQRGLMVRQGQ
                     LLLTDGDMTQAIALPEGWGRVDEPAGAGAALPAREWKTVFGGNPPHVDPGAAFGAVLL
                     YPNDEREIGELATQPFVADYLQDLIEQDRPLAAKVSHAEHVLISGLDAAITTCIGSDR
                     ARAYTVLYDHAAFAQRQAQMWWNLLVRAQRLEWLTRVQMRPRGAEGERRELYDLAYEW
                     TPFSLYDKPESIHHRVQQLASHLSLGAVAFIIGPPTVADSCRTAGVQLQSITPVASLP
                     TFRMHQSVLPRAQLKPGIMLYQIARS"
     gene            13809..14243
                     /gene="gspG"
                     /locus_tag="PLS35_09020"
     CDS             13809..14243
                     /gene="gspG"
                     /locus_tag="PLS35_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNI18844.1"
                     /translation="MNASDGFSFIEVMIVVVILAILATLLIPRVMGRTEDAKRAAAKA
                     QISNIESALQLYKLDNGNLPSTEQGLKALVERPGSGPPAPNWKAGGYLPKVPVDPWGT
                     PYKYTVPSSQGAEFEILSFGADGAPGGDGKNADIVSWDLDRN"
     gene            14386..15579
                     /locus_tag="PLS35_09025"
     CDS             14386..15579
                     /locus_tag="PLS35_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18845.1"
                     /translation="MRRVFFVDDAPEILKHLQRTLAPMQSEWTMEFFSSAKSALAAIC
                     QAPCDVVVSDMTMPGMDGAQFLTEVRKVSPQAIRIILSGDQSPVNYLRSASVAHRFLQ
                     KPVDAEVLKANIAQAEALRAVLANPALRTLVSEIKALPSLPSIYQDLMQEMQSPQASL
                     KKASRIVAKDLGMVTKILQLVNSAFFGLRTHVSDPEQAVALLGFETIKSLVLSSQVFA
                     QFDQNRLPGFSLDALWRHAMLTATCARRIAKDTGASQPVIDEAFTAALLHDVGVLVLA
                     ANKPDDYVRVFELMRANHVSDWAAERDVFGADHAHVGAYLLGIWGLGDGIVEAVAFHQ
                     HPGDYNASGFQAVTAVHVANAFAEAQASADAGTGDVRTLDQNYLTRENLLASIPHWRE
                     LCAAA"
     gene            15661..16998
                     /locus_tag="PLS35_09030"
     CDS             15661..16998
                     /locus_tag="PLS35_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18846.1"
                     /translation="MIQRRTFNALRFITALTHALVLFCVNSSEAGTIMVDLAVEQGTM
                     NHRANGYLVSIEPDEPKMEFILPLKPTSFRGNAGYVFKNYDRLKQAGVTEFQLTLGLV
                     FADLPLQIFDINQIGVDGNYAPWLAHVDRIIDQVMDRRLSVLWDIYNEPDLATLPIND
                     NDRLKEGWRLAYQRIKQRIPGAQIVGPSISRYRLLKPFLEWSHDHNVFPDVVAYHEYD
                     DPSHAPALVEDLRSYLRGRGIVRPISVNEILGQESWTLAGYTVGVIAAYERANILSAM
                     RSCWPDPQDMSRDRVENTCDNPTLDGLLYVDRQSKRPGWHIYKAYAEMAGVRVATMTD
                     SPKLHALASFDKASGTLQLLLGKYEDYSTQDTTVMLKNAARVFSGGQGKNLFLCAEQI
                     PDTGSGPLASTVVTMDRTLPLPDNDISFTLPQFYHSDAYRVRISPTSGCKPRD"
     gene            17276..18016
                     /locus_tag="PLS35_09035"
     CDS             17276..18016
                     /locus_tag="PLS35_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18847.1"
                     /translation="MQDQAAVCTDTPTNQQAGQPPTPQNAVRSWLLRAVALFIKPAHF
                     FATFDDLARPVVLLVATLCLGVASMVDRIEQHILRAEMGQGVSGWSELSPWLLHSWGT
                     LWIALLVCGALNVPLFWYLGGWWYRLRLKWSGATALDSLRPRLLFVYSSLVYALPVVL
                     VMIGETLLFPNYRLARDAEGSWTLIFVLLSFWSVVVSYCGATRTFALARRKALLWFLL
                     LPWTLYAVELGLWMWLFEAFNAAMTETV"
BASE COUNT         3672 a         4959 c         5532 g         3869 t
ORIGIN      
        1 gaagcaggct tgaccgaggc gctgcgattg aaagaatcgc gtgaccggta tcgggaagca
       61 gaacggttag cgcaactagg gcgtgtcacc gaagccatga ccggctatat gcgagccgtc
      121 gagttggacc ccacctataa ggatgcgctc gaaggcgtcg cccgtctgtc cgccgaacag
      181 catgcggccg atcgtgatga tcggcaaaag cagccggtga ccctgcaatt tcgcaatgcg
      241 gggctcaagg aagtcgtaga ggcgctgggg aaggccgctc acgtcaattt cgtgttcgat
      301 aaggatgtgc gaaacgatcc catcacggtc tcgctcgaag aaaaaccctt cgacgaggcc
      361 ttgtcgctgg tgttgaacag caacagcctg tttgcgcaga aggccggccc gacgctcttc
      421 atcattagtc ccaacaccaa gcagaagcag gaacagtatc aggacctgat gatccggacc
      481 ttctatctgt cgtcggccaa ggcgaaagat atggtggccc tcctgaagtc gatgctggat
      541 gtgaaacaca tccatggcaa tgaggccctc aataccatcg tcattcgtga ccaaccggaa
      601 aaggtggagt tagccgaaaa aatcattcag gccaacgacc gcgaggattc cgaggtgctg
      661 ttcgacgtgg aagtgctgga ggtcaaccgg acggttgatc aacagtacgg gctgaattat
      721 cccaagcaga ttggcgcagg gctcgtgcct ccaggattta ccgggacaat cgccggcgac
      781 ctggcgcagc agtttaccta tcgcaatttg gccagcctgg ggcaggacag ttatctgttc
      841 aaggttccca cgaatgtgca gttggatttc tttaagcaaa tcaccgatgc caagacgctc
      901 gccgcgccga aagtgcgagt gctgaacaat aaaaaggcgg aagtgaacat cggcgataag
      961 caaccgattc tgttgtcgac caccaacgtg ttgccggggc aagccgctac cggcgcggtg
     1021 ccgacaacct cgacggtgac gtcgattgag tttcgggaca ccggtgtgaa gctgacggtg
     1081 gaaccgaaca ttcatctcgc gaacgagctg tccttaaaga tgaagattga ggtgatccgc
     1141 ctgggtgaca ccgtcctgct gcagcaatct ccaccgatca cgcagttcaa gttcgggaat
     1201 cgctcggcag agaccatgct gaacgtgcgt gatggagaaa ccatcatcct cggcggcttg
     1261 ctccaggagg aggatcgtcg caccaaggtg accattccat ggatcggcga cattccgtat
     1321 ctgggaaatc ttctgagttc cttcaagacc cagcgggtga cgaccgaagt aatcctgacc
     1381 attacgccgc atatcgtcaa ttcgctccgc attcccgggc cgcaagggca ggcgttttgg
     1441 tcaggaactg agtccgtgta ctccacgtcg ccattatttg ccatgcaacc gaagccgatc
     1501 tcggcgcggg tggcggtctc ggcggcagcc ggatcagcca ttgagaaaag cgggtcgaag
     1561 aagtcaaaaa tgaatgccgc gagttcagac gcggtctcgc ccagacttca gctgtctatg
     1621 cagcctgcgg acgccacagt ccagatggag aaagagatcc gtgtggatgt ggtggcgact
     1681 caggcccaag gtttcgatac ggagaccttc acgctagagt ttgatccgaa gattctcgaa
     1741 tttcgagacg cgaccctcgg tgaagtgttg ggaaccgagg ccggcaaagc tccggtggcc
     1801 gcgacatcat cttccacgga gggcattgtg gagctgcggc tgcatcgctc ttcgactgcc
     1861 accaaagatg aaggccgcct gttgcgattg accttcctcg ccaaatcccc gggtgtctcg
     1921 ccggtgcggc tgcagatggc caaacatccc ggccaagacc tgccggaggg aacgggcgaa
     1981 gcctcaggag tggtgagagt gcggtgaaac agtccggcgt gacgttgctt gagctgctcg
     2041 tgaccctgac gattctcacc atccttgcct cggtcgcgct gccgttcacg aaggtctcca
     2101 cgaaacgcac gaaggagatc gaacttcggc agaatctccg ggtgatacga gccgcgattg
     2161 atgcctttcg cctggaatgg gctcgtgacg gtgacacgct tatcggtccg gcctgcgtca
     2221 agaatcggtt gagttgcaaa gacgttaccg gcccctacgg atatccgaag tcgctcgatg
     2281 ctttgctggg ggtcaaactc accggtgagc aggcgacggt gcggggaacg acgatacggc
     2341 gctatctgcg ctcaattccc atggatccga tgaccggcgc agccgattgg cgcctgcgtt
     2401 gctaccggga ccccgccagc gtgaaagact ggtgcggaga agacatttac gatgttacga
     2461 ctcagagtca agaactggcc ttggatggta cgaaataccg agagtggtag gcgcgcatgg
     2521 gtctcctcgt cagcgcacgg atttacgatt atcgagttga tgattgtggt gacgatcgta
     2581 gggattcttg ccacattggc ggtgccgtcg tatcacgcgg cgatcattaa agcgaaggaa
     2641 ggggccttgc gtcaggattt gttctcgtta cgcgacgtga tcgaccaaca tcgcgccgac
     2701 aaggggaagt atccggagag cattcaagcc ctggtatcgg ccggttacct caggagagtc
     2761 ccgaccgacc cgttgaccgg ggcgaccaca tcgtggcagg agatggtcga tgaagccgaa
     2821 ggcgggatgg tcgatgtgtt ttcaggctcc gaactggtcg gaaccaatgg agtaccctac
     2881 aaccaatggt aaggcaatga acatcatgat acagccacat gttcctgcgc gacggaaggg
     2941 agttgggtta cgtctcggat gctggttggc cgcattgctc ctctgtgagt cctggggagg
     3001 ctgcgcgaag gtcacgccca atcacgaacc agccccctcg gatctgcaag tggcggcgga
     3061 caccctcaag gtcgcggttc gcgaagcgca gcggacggcg gctgagttgc ggacggaact
     3121 ggaggaacag agaaaagaac tggccgacgc gcaggtggcg cgtgcacaac tacagggcat
     3181 gttacgggaa acagagcggc gactgacgga ggcccgtcag atcatcgaat tgcagcgtga
     3241 agagctcacc tcagctcgcg acgagcggga acgattggct caggcggtcc acccctttac
     3301 cgggcatgtg aaggcatcct ctgctcctgc tccatcgcgc agcaagaggt cacccgctgc
     3361 ccagggtgcg gcgattccgg gctccgtcga cacgcgggag atgagggatg acaattccct
     3421 cgtagagcaa gaaccggcgg ctccgcagcc tctgcctctt catcgcgagg ccacagactc
     3481 atcgacggct caggcatcag ccgttcatgt cgccccgctt ccgaaaggaa aggccgtggg
     3541 tcggacgatc gtggtgcaag gcggcgatac gttatggggg ctcgcgcgcc ggcacaaggt
     3601 tgaagtcgcg gtacttcgcg cactcaacgg tttgtcaggt gaccgcatcc tatctggacg
     3661 aaagcttcgt ttgcctgagt cggaagcgca gcatgcggct gtgcaaccgg ctgcagtcga
     3721 tgtcgtggtg caatagcggt tgaagattat cgcgtgatgt gccgagccga atgaactcat
     3781 ggctgttttt tcctatagag cggctcgcgc ggatggaacg accttcgaag gccacatcga
     3841 aggcgacgat gagcatgtgg tccgcgcgaa actcgaagca gacggcgtcc tggtctttcg
     3901 gctctcccga agcggaacag ggtttggtgt gccgggcctg tctgcgagcc gctggggcaa
     3961 gcttcccctt caagattttc tcgtgtttaa tcaagagctg ctggccctaa tcaaggcggg
     4021 gctcccggtt cttcgggtgt gggatcttct catcgatcgc acgcagcgcg cgccgtttcg
     4081 agaagcgctc aaggccgttc gtcaggatat tcgtggcggg gcctcggcat ccgaagcctt
     4141 ggcgaaacac tccacctatt tttcggatct ctatttggcc accattcgcg ccggcgaaca
     4201 gtcggggaat ttggcggagg tgttgcaacg gtacattgcc tacttgaagc tgatgatcag
     4261 ccttcgccaa aaggtgacga aggccttggc atatccgggt tttctcgtcg tggtcggcgt
     4321 tgcggtggtc ggatttctcc tgagctacgt catgccgacc tttatctctg tgtacggaga
     4381 atcgtccgca aatctcccga cgccgacgcg attgctcatc tccgtcattc atatgggaga
     4441 ggcgcaattg attccagtgg ccattatcgt cattgtagca accgtcatgg ggcgggcttg
     4501 gtatcaaaca tcggccggac ggttatcggt ggaccggaat ctgttgcggt tgcccttact
     4561 cgggaccatt ctcgtggagc atcatacgat ccaattgacg cggacattgg cgacggtgtt
     4621 ggccggcggt acgccgattg tggaagcgct ggaaattgcg cgcggagcgg tctcgaaccg
     4681 cttcgtctca cgcggactgg tgtccgcggt caatgagatc cgtgaaggca gcacgctggc
     4741 ggcggccatt gagaggccca aaattttgcc caagctggcc atcgaaatgc tgtcggtcgg
     4801 ggaggagaca ggctcgctcg agccgatgct cagggatgtg gctgaatttt atgaaggtga
     4861 tctcgatgtg cggctcagcc agttgacgac gtggatcgag ccggtcttgt tgctggtcat
     4921 ggggttgctc gtcggcggaa ttgtcatcat catgtacttg cccatttttc agatggccgg
     4981 aacgattcaa taatgcggcg gggtagacct catcgttcag gagcctggag tcccatcagg
     5041 ggtggagatt tgtagtatgg ccatgcaacg tcatctcacc cgcccgtcgt tggccgatgt
     5101 gttggtgcgc gaagggatcc ttcctcgccg cacggtcgat caggtcgtgg cccgccttgg
     5161 aggcagcacc accgcgttgg gacccacgct ggttgaagac ggcaccattt cagagatgca
     5221 gttggcgcaa gcgttagccg cgcaatatgg ccttccttat gacgcgctga ccggcttccg
     5281 ggtggattcg gagttttatc acaccatttc cgtcaagctc atgcgccgtc atcccttcgt
     5341 gcccctgaaa gacgaggcgg ggatcctcac cattgcgatt tccgatcccc agaacctgct
     5401 gggcctggat gaattggaaa tcctgctcaa tcgtccgttg cactatgtgg tgagttcaaa
     5461 gaacgcgatt caggccgcac tggagcgcag cgaaggatcg agtcaggcct tacgtgaatt
     5521 agaggctgag taccggtcgg ttctcgtcaa ggaagacgag cggggcgaag aagtcctgac
     5581 ggtagaccat ttcggcgagg accaaagccc cgtcgtcaag ctgctcgaca cgatcatgtt
     5641 aagcgccatg cagcggcggg cgagcgatat tcacattgaa gccacggatc gtgcgacgac
     5701 ggtgaaattt cgcgtggacg ggattctggt ctcagccatg gacccgctgg atgtgaagct
     5761 gcacccgccg ctggtctctc gcctcaaggt catgtccgat ctggacatcg cagagcgccg
     5821 ggtcccgcaa gacgggagtt tccggatgcg ccttgaccgg aaaacggtcg acttccgcgt
     5881 gtcgattctc ccgagcgtgt tcggcgaatc ggtcgtgatc agaattttgg accgcgaggc
     5941 gatcaccacc ggcgtctcca ccttgcggct ggatcggctg ggattcaatc ctgaagatct
     6001 caagcggttc cggcgcgcga ttacgaggcc ctacgggatg gtgctcgtga ccggtccgac
     6061 gggaagcggc aagaccacga cgctctacgc cgccatcagc gaaatgaaca ttcaggaaga
     6121 caagctgatc accatcgaag acccggtcga atatcagttg ccgggtgtcg tccaaatccc
     6181 ggtgaatgag aagaaaggcc tcacctttgc ccgcgggtta cggtccattc tgcgtcacga
     6241 tcctgacaag atcatggtcg gagaaatccg tgatgcggag accgcgcaga tcgccattca
     6301 atcggcgctc accggccatt tggtgttgac tacggtgcac gccaacaatg tgttcgatgt
     6361 gatcgggcgg ttcgcttcga tgggcatcga ttcctataat ttccttgcgg cgttgacctg
     6421 cgtgttggcg caacgattga tccgggtgat ttgtccggac tgtcgccatc aggtcacgct
     6481 ggatcaagcg ctggcggaag agtccggtct cgattatgac gaattcaaga cctcgccgtt
     6541 ctatgaaggc aaaggctgct ccgagtgtca tgacacgggg tatcgtggcc gcaagtgcat
     6601 taccgagttt ctggacctga cagacgagat caaggaaatg attctggctg accgtgcatt
     6661 gtcggagatc cggtaccggg cggtcaccga cggcatgatt acgctgcgtc aatctgcggt
     6721 caagaaagtg ctggccgggg agacgacgtt gcgcgagatc aaccgtgtga cgttcagtga
     6781 agagcgatag acgatgtggg attggattac cagcagaccg cagtattgct tgaaaatcgg
     6841 cgcgcatgat ctggcctggg cggaggtgca tcgcaattgg cgcgggcgtc cacggtatca
     6901 atgcgtggtg tccgcgttgc cagatggcat cctccggcta tcgccattgg aacagaacgt
     6961 cgtgtctccg catgaactgg aatcgcatat ccgcgcgatt gccgggtcgg cgacgaccca
     7021 agctcctggc cgttcttcca ccccgggagt cccgcgcgcc gccacggtga ttctgccgga
     7081 tctggccgtg cgtctggcgg ttgtccgctt gcaagacctt ccgtggagtt cggatgagcg
     7141 ggatgcgttg gtgcgttggc gattagggca ggagcagttg ctgtccttaa gtggggccaa
     7201 agtcttctcc caagtgttgt ccgaacccgg cgtgtccggt gacgggccct gcaccgtcct
     7261 cgcggtggtg gtgcaggagg ccgtgctgtc gcagtatgaa gccgtgtgcg aagccgccgg
     7321 tcttatcccg caggaagtgg atgtcgccag cctgcgcctg ttcaacctgt ggtcccatgg
     7381 agcctggcgt accgcgggct cgactgccga ttttttgtgg gtgaatgcca ccgagggcgg
     7441 ggtcacggca tttatctttc ataagggcgt cttggtcttc ctccgttcca aattgcaggg
     7501 gggcgtcgga tccagtggga tctggcctgc cggctcagag cagttgggga tcaaccggat
     7561 cgtgcaggag tgcgccgact cgatttacgc ctgtcagcaa cagacgcctg aattagcgat
     7621 cagccaaacc attctaattg cagatgacgc cctgggaccg ggcttgcgcc agcaattgga
     7681 aaaaggatta ggcgtgcccg tgcaggaatt ggattgggaa cgggtgcaac aattcggcgg
     7741 tggtccgatt gccgggccac ggacaagcgc ggccatgccg gcgatcgcag gggttgtgta
     7801 agtcatgatg ttggataatc tggcatcggt gcttcgcgat gtggcctcgc gtatcggtca
     7861 accgtctgcc cgcagcggtg tattcacgat caatctgagc agccgctatc gttggtatct
     7921 ggcgccggca cggttgctga ttatggtatt ggcgggagtg atcggcgtgg ccatcctttg
     7981 ggacatttct caatcttggc tggtgtggca agacgtgcaa gcgcgcgaat cagccctgtt
     8041 gcaagtccag gagcgggaca acgaattgct caaggaggcg cagcaggaag gcctggacct
     8101 ttccgaagcc gccctgcaat tgctgccgaa agagatcgag ttcgccaatc atctgatcga
     8161 gaagcggagc ttttcatgga cccggttcct gacggaactg gagcgtgcga ttcctcagcg
     8221 cattgccgtc aacagtattc gtctcgatcc cgcgaatgcg acgatcatgc tgaccggatc
     8281 ggcgagggcg ctggaagatg tgaccacgct gacggtgatg tttcaagacc atccccaatt
     8341 taaggatccc gtgttgggcc agcaccgggt gatgggaaac gggctggtcg agttcgacct
     8401 cacgttgcgg tatcgaaacc ggagctaacg atgtttccag gcctcgatcg cctacaattg
     8461 accgggcgtc aaccctacgc gcccatcctt ccctgggtgg ctttcgtggt ggtcctggcc
     8521 atcgtgagtt acggcattca cgccctggtg ttgggtgcgg cggaggagga gcaggtgcgg
     8581 ctcgaagccg agtcggtcaa ggcgcggcag cagttgaacc ggcacaggga agccaagaag
     8641 gcgaaggcgg atcttcagcg cgtgtgggcc gtcctgccgt tgtttcggga ttttgccccg
     8701 ctcgccctcg gtgtaacgga agaagccaag cgggatcagg tcacgctgcc ggctctgtcc
     8761 tataagacag agaagacgtc ggtaccggat gccaccaagg cggtgctgca ggggacggtg
     8821 accgggcggt atgaggactt gcgccgcttc ctctacaact tggagtcggc ccaggaacta
     8881 ttgtttatcg aggatctcaa tctcgtccgc tcaggaaaca ttcaggacca gcaattgacg
     8941 ttcaatatca ggatttcgac ttatttgcgg ggagagcatc tgcaatctcc gacaccctga
     9001 cgctcatgaa ccgagtcagt aaaaatcagg ggatgctgtt gctgaccctc gtcgtcttct
     9061 ggatcgggtt gctcatctgg caatttgacc attcgaacga acaggcgcga gtcccgttga
     9121 cccatgtcag tggacagcga gccacgagta agacgcctca agccgcttcc agtggtaatt
     9181 tacaagtgca tctggagcgc ttggccgcca tcaagggcca gcgcgaagcc acgttttcca
     9241 cgccgcgcaa cattttcgcc agcctgctgc cccaggtgga gccggtagcg gttccgcctc
     9301 cggctccccg ccgaggccgt tcacgtgggc ctgctaaggc agctcctcct cccgtacttg
     9361 agccgctcgg ggagccacag gaacagactg ccgctgaaga ggagcaggaa cccgtggtag
     9421 aagggccaaa ggggccgacg ccggaagaaa ttgaacggct ccggatcgcg accgagctca
     9481 accagtaccg ctatgtcggc ttcatggcgg ttggaggagg atcgcagaaa aagaagaaca
     9541 tggcggtggt ggtcaagaat gacgagatgc atgtcgtgca ggtgggagaa gtgatcgggg
     9601 gagaagtcct ggtcaaagat gtgtcgttga aggaaattac gcttcaagat gtgggatcaa
     9661 aaatcaccca ggtcattccg gttacggatg atcccggcgc aggggggcaa cccaactaag
     9721 ccggcgatgg ggttgcgggg tcctctccga aatgcccggg gtatcaccta tctggccttg
     9781 atgttttcga tcgtcttgat cggcatttcc gtttctgccg cggctcgcca gtggacggtg
     9841 atggtccagc gcgaaaaaga agccgatctc atggcaaaag ggatcgaaat tcaaaacgcg
     9901 ctggcgttgt actcggcctc gatgaaaatc gggcgcatca tgacgaccga aatctatcct
     9961 cagtctctag ccgaattgac gcgcacgccc aaaccatttt tgcgacgagt ctatcaggac
    10021 ccgatcagcg ggggagactg ggagtacatg cgagcgccca ccgggggcat tatgggggtg
    10081 cgcagcaaga gtaaagtcaa accgatcaag gagcgtgatt ttccgctggc cgtccgccat
    10141 ttcgaagggc gcaagaccta ccatgactgg atgttccagc accccaatcc gtcttcgcaa
    10201 tccatgctga tgccgcccat gatggggctg gcgcccggga ccatgccgac acaccccggt
    10261 gcacaagggg ccgttcccgg cggcgggaca ccacaaggcg ctcctctgat gccaggggct
    10321 ccccgtaacc cttgacccga ctccaaaccc cacagtatcc tgtcacgcta ttgttttttc
    10381 gcctacgttc accatcttga tcggtggata cctttggcct cgggcctctg tatcgccaag
    10441 cagcaggaat tcacgacatg gaacaagacc tgaccgaatc cccggctccg gagctggaag
    10501 ctcccgttga agtactgtct cctgaagact atgtgatggg tttggtgaag agcgccaaga
    10561 aggcggcggg ccggctgtcg accctttcgg ctgcagtcaa aaatgaagcc ttggaggcta
    10621 tggctgaagg gcttgtggcc catgaggcgg agctgctggc ggcgaacgaa aaggatctcg
    10681 aacaattcga tgccacgccc gagcgcaagg cgctaggtga tcggttgcga ctgacggcgg
    10741 agcgtattcg cgatatggct gctggcttgc gtgatgttgc ccgcctgccg gatccgcttg
    10801 gcgagatgcc caagatgtgg acgcggccga acggcatgca ggtgggccgg atgcgggttc
    10861 ccatcggggt catcggaatc atttatgagg ctcgtccgaa cgtcacggcg gactcggccg
    10921 ctctctgtct caagtcggga aatgtctgtc tcctacgcgg cggatcggaa gcgctgcatt
    10981 ccaatacggc gattgccaaa gtgttgagcg aatcagcgga gaaggccggt gtgccggccg
    11041 gcgccatcac ctttgtagat cggccggagc gtgaaatcgt ccagctgttg ttgaagcagg
    11101 atcagtatat cgacctcatc atccccaggg gcggggattc gttgatgaag accattgctg
    11161 aacatgcgac gattccggtg ctgaagcatg ataagggcgt ctgtcacacg tatgttgatg
    11221 cggatgccga cccgaaagtg gctgaacaca tctgcatgaa tgccaaggtg cagcgaccct
    11281 caacgtgcaa tgccatggag acgctgttgg tccaccacag catcgcacgg acctggcttc
    11341 cgcatctcat caaacagctc acggctgcga aggtggaagt gcgtggttgc gcaaagacct
    11401 gccagctctg tcctgaggtc aagccggcag tggaggatga tttcgggcat gaatttcttg
    11461 atctcattct cgcggtcaaa gtggtcaaac atatggatga agcgctggag cacattgcca
    11521 cattcggctc ccgtcatacg gaagcgattg tgaccaagga ttattcccgc gccatgcgtt
    11581 tcttgcgcga agtcgatgcg ggtgcagttt tggtcaacgc gtcgaccagg ctcaatgacg
    11641 ggtatcagtt cggcctcgga gcagaaatcg gcatcagcac ctcgcggctt cacgcgcgag
    11701 ggccgatggg gctggaagaa ttgacctgtt ccaagttcgt ggtgcttggg agcgggcaga
    11761 tccgcgaata aatgcccgtg gatgtctcgc tacaggagat actgagtcgc cccggtacgc
    11821 tcgcctttcc ttccaatctt gctgatgcgc cgcgcttgcc ggcgtcatca gcgtcgtgcc
    11881 ttcgtctgcc cacgggacat ctcgtcgtct atggaagcca tgggcggcga atcctagcga
    11941 cggatccgga cgggtatcca ttgcatgagt gcgaatggga gtcggtcaat ggccggctgc
    12001 gtctcgttcg aagccgggta cacctggatt ggggggcatg ggttggactc attcccggcg
    12061 ggctcgtcaa tacgatgacc ctggatctgt ccaggaagcc tggatggcag cggctcaggg
    12121 ccgacgatct tcgaagcatg gcggctcaag ccatgcatgt cccgctcgat gaagtccggt
    12181 ttttttacag cgatcaggac atgacgattg atgccaaggg tcgcgccacg atccggcatc
    12241 gcaaagatgc catctaccat ctgcctgatg ggaccttcga caccgaacaa tttatggcgt
    12301 gcatgggggc catgcattgg gaagccatcg attttctgcc ggtcgtcgag ctctttttat
    12361 cgctgctgcc cggcactggc tccgcactct ttgaattgat ccgtggcctc tatgatgatc
    12421 agaatcgcgg acaagttgct ccaaggccgt tgcgataccg tggtattccg acctatccgt
    12481 cagaagcagc ctatcgtctg ttcagtaatt tcttttctcc tcacctgacg gacggcagtg
    12541 atccctttcc ggtgttcatg gatacaccgc gatcccacct ggtgacatgg accccggtcc
    12601 cggacccgcc gagaagacat gtcgacctgt cccagaaatt gtgcgtcacg attcaaggtc
    12661 ggcgaatgct gaaagcaacc cggtgggatg atccggcagg gctttcctat cagcccttcg
    12721 atcagagagg cctcgcaccc tgccagcgcg gattgatggt ccggcagggg cagctcctgt
    12781 tgaccgatgg agacatgacg caggcgattg cattgcccga gggttgggga cgcgtcgacg
    12841 aacctgccgg ggcaggtgcg gccttgcccg cccgtgagtg gaaaacggtg ttcggtggga
    12901 acccgccgca tgtcgatccg ggagcggcgt ttggtgccgt attgctctat cccaacgatg
    12961 agcgggaaat tggggaattg gcgacgcaac cgtttgtcgc cgactacctt caagatctca
    13021 tcgagcaaga tcggccattg gctgcgaagg tatcccatgc ggagcatgtg ctgatttccg
    13081 gactcgatgc ggctatcacc acgtgtatcg ggagcgatcg tgcgcgggcc tatacagtgt
    13141 tgtatgacca tgcggcgttt gcacagcgtc aggcgcagat gtggtggaac ctcctggttc
    13201 gggcgcagag actagagtgg ctcacgcgtg tgcagatgcg gcctcgtgga gcggaagggg
    13261 aacgccggga gttgtacgac ctggcatatg aatggactcc gttcagtctg tatgacaagc
    13321 cggagtcgat tcaccatcgt gtgcagcaat tggctagcca cctgtcgctt ggggcggtgg
    13381 cattcatcat cggcccgccg accgtagcag acagttgccg aacggctggg gtgcagctgc
    13441 agtcgatcac gcccgtggcg tctctgccta cgttccgcat gcatcaaagt gtgttacccc
    13501 gcgcgcaact caagcccggc attatgctat atcagatcgc tcgctcctga tcggtggcct
    13561 catggcagat gcgtgtatag agacgcgtac gagagcgctg acaggagcat gtcacctcgg
    13621 atttgaatga cacactcata gacgttcctg ttacaattca aaattggtcc acgcatggag
    13681 ctcgcagcga gccggatcgg ttgagagaag gagcagcacg tatggggttt tttcgagaat
    13741 acaggaagtc tacagggcta gaccgcggtg catcgtgcga gcatcgttgc tcaggtcttg
    13801 caggaagact gaacgcgagt gacggatttt cgttcatcga ggtaatgatc gttgtcgtga
    13861 tcctggcgat ccttgcgacg ctgctgattc ctcgagtgat gggccggacg gaagatgcca
    13921 agcgggcggc cgcaaaggca caaatttcaa acatcgaaag cgcgctccaa ttgtacaaac
    13981 tggataacgg caacctcccg tcgactgagc agggcctgaa ggcgttggtg gaacggcccg
    14041 gatcaggtcc ccctgcgccg aattggaagg cgggtgggta tcttccgaag gtgccggtcg
    14101 atccgtgggg cactccctac aaatacacgg tgccgtcttc gcaaggggcc gaatttgaga
    14161 ttctctcgtt cggcgcggat ggcgctcccg gaggcgacgg caaaaacgcc gatatcgtca
    14221 gttgggatct ggaccgcaac tagtgtctgg agtcaggccc gctgagttct ctttccaatc
    14281 cacaggattg cctcatctcc ccgttgtagt ggttcaatct gacttaagat cctgcccctc
    14341 tcgaccgatg agaagaagag acgaatcctg ccgggaggag ggcccatgcg acgcgtcttt
    14401 tttgtcgacg atgcgccgga aattctcaaa cacctgcaac ggacgttggc ccccatgcag
    14461 tccgaatgga cgatggagtt tttttcctcc gccaagagcg cgttagctgc gatctgtcag
    14521 gcaccttgtg acgtcgtggt gtcggacatg accatgccgg ggatggacgg tgcccaattc
    14581 ctcaccgagg tgcgcaaggt ttctccacaa gcgatccgca ttattctctc cggcgatcag
    14641 agtccggtga actatctgcg ctcggcctcg gtcgcacatc gctttctgca aaagccggtg
    14701 gacgcggagg tcttgaaggc gaacattgcg caggcggagg ccttgcgggc ggtgttggcc
    14761 aatcctgctc tccggacgct cgtcagtgaa atcaaggctc tcccgagtct gccgtcgatc
    14821 tatcaggact tgatgcagga gatgcagagc ccgcaggcct cattgaagaa agcctctcgc
    14881 atcgtggcca aggatctcgg catggtcacg aagatccttc agttggtcaa ttcggcattt
    14941 ttcggcctcc gcacgcatgt gtctgatcct gaacaggcgg tggcgcttct gggatttgag
    15001 acgatcaaat cgctcgtgct ctccagccag gtctttgcgc aatttgacca gaatcggttg
    15061 ccgggatttt cactggacgc gctgtggcgg cacgcgatgc tgacggcaac ctgtgcccgc
    15121 cggattgcca aagacaccgg tgcaagccag ccggtcattg acgaagcgtt taccgcggcg
    15181 ctgctgcacg acgtaggcgt gctggtgttg gcggcgaaca agcccgatga ctatgtgcgg
    15241 gtctttgaac tgatgcgtgc caatcatgtg tcggactggg ccgccgagcg agatgtattc
    15301 ggagctgacc atgcgcatgt cggcgcctat ttgttgggca tctgggggct cggcgacgga
    15361 attgtcgagg ccgttgcctt tcatcaacac cccggtgatt acaacgcgtc tggttttcag
    15421 gccgtgactg ccgtgcatgt ggccaatgcg ttcgccgagg cccaagcgag cgctgatgcc
    15481 ggcacgggtg atgtacgcac tctggatcag aactacttga ctcgtgaaaa ccttctcgcc
    15541 tccattccgc attggcgcga actgtgcgcg gccgcctaat ccttcttcct ttggcttgac
    15601 cctcgctcct gtcccgcagt acaacgacat ttcgtgtgat gcatttcgga tcgaggaagc
    15661 atgatccaac gtcggacatt caacgcactt cgcttcatca cggccttgac tcatgcgctc
    15721 gtgctgttct gcgtcaactc gagcgaggcc ggcaccatca tggtcgacct tgctgtcgaa
    15781 cagggaacga tgaaccaccg ggcgaacggg tacctggtga gcatcgagcc cgacgagccg
    15841 aaaatggaat tcatccttcc gctgaagccc acgagtttta gaggcaatgc cgggtacgtc
    15901 ttcaagaatt atgatcggct caagcaggcc ggggtaacgg agtttcagct tacccttggg
    15961 ctggtgttcg cagacctgcc tctgcaaatt tttgatatca atcaaattgg agtggatgga
    16021 aactacgcac cctggctcgc gcatgtggat cgaatcatcg atcaagtgat ggatcgtcgc
    16081 ctctccgttt tgtgggatat ctataatgaa cccgatttag ccacgcttcc tatcaatgac
    16141 aacgatcggc tcaaagaagg ctggcggctg gcctatcaac gtattaaaca acggattccc
    16201 ggtgcgcaga ttgtggggcc cagtatcagc cggtaccgac tgctgaagcc ttttctcgaa
    16261 tggagtcacg accacaacgt gtttccggat gttgtggcat accacgagta tgacgacccg
    16321 tcacacgctc ctgcgctcgt ggaggattta cggagttacc tcagaggtcg aggtatcgtc
    16381 agacctattt ctgtgaatga gattctgggg caggagagct ggactcttgc tgggtacacc
    16441 gtcggcgtca ttgcggcata tgagcgcgcg aatatcctga gtgccatgcg atcctgttgg
    16501 ccggatccgc aagatatgag ccgcgatcga gtggaaaata cttgtgacaa tcctacgctg
    16561 gatggtctgc tctatgtcga tcggcaatcg aagcggccgg gatggcacat ctacaaagcc
    16621 tatgcagaga tggcgggggt gcgagtcgct acgatgaccg acagtccaaa actccatgcg
    16681 ctggcgtcat ttgataaagc ttccggtacc ctccagttgt tattaggcaa gtacgaggac
    16741 tattcaaccc aggacacgac tgtgatgctc aaaaatgccg cccgggtctt ctcaggcggg
    16801 caaggaaaga atctctttct ctgtgccgag cagattccgg acacgggatc cggcccgctc
    16861 gcctcgaccg tggtgacgat ggatcgtacg ttgccgcttc cggataacga catctccttc
    16921 accttgcctc agttttacca ttcggatgcc tacagggtga ggatatcgcc cacatccggg
    16981 tgcaagcccc gcgattaacc actccaccgg ggtggtgtca ggagtatcta caagggatat
    17041 cgaccccggc ggcatccctc aagcgctatt ttcgtcaaag cctcagggct aggttcgtcc
    17101 ccctccttcg agcagcccct tccccaacct ggacaattgt ggcctcacgg catcccgctg
    17161 tacgctccct ccaaccatcg ataggcgggc gagcgtaatt cggcgctgtg ccggattgtt
    17221 gcgtatcgat ggacgagtcc agtccacgac cagcgaagaa aaggtatccc tccttatgca
    17281 agaccaggcc gcagtgtgta ccgatacgcc aaccaatcaa caggctggcc agccgccaac
    17341 ccctcagaat gccgttcgat cctggctgct tcgcgcggtg gctctcttca tcaaaccggc
    17401 acactttttt gcgacgttcg acgatctcgc acggccggtg gtgctgcttg tggccacgct
    17461 gtgtttgggt gttgcttcca tggtagaccg aatcgagcag cacattctcc gtgcagaaat
    17521 ggggcaggga gtctccggat ggtcggagct gagtccgtgg ctgctccatt catggggcac
    17581 actttggatc gcgcttctcg tgtgtggggc tctgaatgtg cctctgttct ggtatctggg
    17641 cgggtggtgg taccgccttc gtctcaagtg gtctggagcc acggcgttgg attcactccg
    17701 gccacgtctc ctgttcgtct attcttcgct ggtgtatgcc cttccagttg ttctcgtcat
    17761 gataggcgaa acgctgctgt ttcccaatta ccgcctggcg agggatgcgg aggggagttg
    17821 gacgctcatc tttgtgttgc tgtcattctg gtcggtggtc gtgagttatt gtggtgcaac
    17881 caggaccttc gcccttgcac ggagaaaggc gttgctctgg tttctcctcc ttccctggac
    17941 tctgtatgcc gtagagctgg gtctgtggat gtggctcttt gaagccttta atgccgccat
    18001 gacagaaact gtctgacctg aagtctgatg ac
//
LOCUS       DAOQEW010000066        17948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_815_length_17948_cov_7.984407, whole genome shotgun sequence.
ACCESSION   DAOQEW010000066 DAOQEW010000000
VERSION     DAOQEW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17948
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_815_length_17948_cov_7.984407"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLS35_09040"
     CDS             complement(<1..187)
                     /locus_tag="PLS35_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18848.1"
                     /translation="MDCQWFTRVTSRALSVIAGGALVCLPLAASAESQLHADHAVPQQ
                     TAMSHQLPSWAEKLKGQT"
     gene            550..2364
                     /locus_tag="PLS35_09045"
     CDS             550..2364
                     /locus_tag="PLS35_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNI18849.1"
                     /translation="MILKRWLVGLPLKTKEAAHERLSKRLALAVFSSDALSSVAYATE
                     EILLVLTLAGTAMVGYSIPLSLSIIGLLVILTMSYRQIIFEYPEGGGAYIVGKSNLGE
                     WPGLVAAGALMIDYVLTVAVSVAAGIAALTSAFPDLLAHREALCVAAILLVTVVNLRG
                     VRESGQFFAVPTYIFIGTLTAMLGVGAIQILLGHATRIEPLPSLTPAEPLTLFLLLRA
                     FSSGCTALTGVEVISNGVSAFKKPEAKNAAFTMIGMAVILGTLFIGISAMAYYFGIVP
                     KSDETVVSQIARATFGTGLLYFLVQASTMTILILAANSSFNGFPRLASILARDSYMPH
                     QMSMMGDRLVFSNGVIILGVFSCLLIILFNGDTHALIPLYAVGVFLSFTISQAGMVKR
                     WLVKKGPHWEKKLLVNGIGAVTTAIATVIIASTKFAHGAWIVIVLIPMLIMFFKAIRS
                     HYKAVSEQVALSRGHRPPLPRRNIVVLPIGGVNRAVIRAVDYARSRSGDIRAVLVDVD
                     PEETARVEIQWAQWGCGVPLTVLPSPYRSVLSSLLDYLEQVLQKDQECWVTVVIPEIL
                     PARWWQNILHNQRAFMLKGALLFKDRVILTDVPYHLTR"
     gene            2367..>3388
                     /locus_tag="PLS35_09050"
     CDS             2367..>3388
                     /locus_tag="PLS35_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18850.1"
                     /translation="MRNPRATIHRTNRRVGAHLVAMVLLLGLSPHPGLAFTIVAPKNG
                     TTLTSGEPTTAEVEAGREAGLVEVRYYWYPEQTESLVEQGDERSGKGSQGSISTEKYW
                     QKDSITGAPVVALPALTSTVDRVPPYGGALPVPPDAVGRMRLLAIADISQGRLGRKTV
                     FDEVFVTVQPKAELLSIDFETEKPLQLGRTGQSSAYGHVDSLGKIFELPVVGEFADGI
                     SRPLSSPSAGTVYTSGNDQILKVLPDGLLQIVGIGKTSLTVTNRGKQATLDVRVEVNP
                     EPNEPPIANAGTAKTVKAGTKVRLSGLQSRDPEGEALFYAWAQVRGSKVAMLDVNMAE
                     PSFTAP"
     assembly_gap    3389..3488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3686..4216
                     /locus_tag="PLS35_09055"
     CDS             3686..4216
                     /locus_tag="PLS35_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18851.1"
                     /translation="MALIQQDLVAGSEDRLLTLDTETNLQWLNLSQTAGRSYQEIMNG
                     FGQFTTAHGFHYADGRQIGELCGHAGITKGLTEPALTPSPNDARNHQAIQTLQNLMDG
                     KVFHAETNVITSRGIMKPPAPPPNVPTRILGTIRLSLSLLNITGSHAESEGPTASPQT
                     GDPEIGSYLVRNQPEA"
     gene            complement(4354..5901)
                     /locus_tag="PLS35_09060"
     CDS             complement(4354..5901)
                     /locus_tag="PLS35_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HNI18852.1"
                     /translation="MTVLDARMLERLMEEFRRSMQGLLTEVCRDFTKNYADAVSTFQL
                     PIDWFLSLGRSFPPAEFSNWKVVGWIESLNDLLYFVDIVTQVRQERSRRDIAEQLRAE
                     FREKFYEHGYTDEIFPNGKPEPRLLLGRLTALCHRLAREITQESVCLAPRLACAWVAK
                     QRRDAWLLPCDLTANVERVELPWGCAIGTTGVFYEATGAVRSALQRAKGQGEFLITAS
                     GIELLVGDRAYPLMTCGSQERWHWRRLDPLPLRDSRYGSLVLGPTLVYGKDKTPIGVR
                     PTKQEVAVRMRRALSAIAAAWPEGDRLLALLTSRIVPLKAAGVVSFSYRHRPGFSAIN
                     CFERDQLDLIDDLIHENSHHHLNLLLRNDVMYQHDHNQEVFYSPWRRTLRPLRGILHA
                     TFTFTMGAMLFERLVVWGSGTSGKAGWTKAGLTQRDLARARYRCLEEIESVRYSLQDL
                     EYAGKHLKWLTRGGKRLVTQLRDALTSMERGIQEYEQRVSASAFGPALRRHRIELAQA
                     RETLSLM"
     gene            complement(6142..6369)
                     /locus_tag="PLS35_09065"
     CDS             complement(6142..6369)
                     /locus_tag="PLS35_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18853.1"
                     /translation="MTCTRCEGLMVMDDLVDLQESYHPMRLRGHRCLSCGNVVDTLIQ
                     RHRMVQNSHLRPVPASVPEKPVLFELIRRSA"
     gene            6699..8156
                     /locus_tag="PLS35_09070"
     CDS             6699..8156
                     /locus_tag="PLS35_09070"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNI18854.1"
                     /translation="MALTATEIGSIVQELASALADGWIQKISQPLPDTLILEIRVPGH
                     TRRLLYSLRDDTARLHLVHQHLPNPPTPPSFCQLLRARIQGARIDTLQHMSGDRIVRL
                     DLTSRDGPASLVAELFSRNADLLFLDGEGLVLATLRHNKDRVGQPYQAPAAAPFRSSS
                     HQAVVHEPDTQPQTDVDPFPLSSRLEAFYREREAELSHQAQARERESGLRKHLKKLLR
                     RMAALRQDLEQAGRYEPYARYGELLKANLGLLKKGQNRVAVVDYYDEQLPELTIPLDP
                     TKGPQANMDAYFAKYRKFVTAQREISPRIAAIEAEVQQILTELETIKQGTWQPPSTEG
                     QTAARGTPALRRKRDREGESRGPFRRFVSSDGHPILVGRNARENDELTFGLAKSEDLW
                     LHARGTPGSHVVVRLDKGTEPPPETIRDAATLALLYSDLKKSGKGDVIYTRRKWVKKA
                     KGQAAGAVTVTQEKSIYVTLDKPRLDALKSRSAPR"
     gene            8265..10307
                     /locus_tag="PLS35_09075"
     CDS             8265..10307
                     /locus_tag="PLS35_09075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI18855.1"
                     /translation="MGTAFDTRDWFPILSALLQTNTLRQFDDRLQRELSQRLGCSVIG
                     LYLYSEMATAFSPVSESLRDPASPAYPIAQLPASGTIKEAAVLAGEAILTGDLSTSPW
                     TEARAIAPHISHGTSVLVSPIRMSCESVTGTAAKTLGVIAAVAPGKPGAFSEEDRIWL
                     DQLSRHIAPVLTAVLAAEERDTLVAINNQVVLGTLTLDNLIHSIQPILREVIHHDITG
                     LVRFVGEPHNRWFDIVACEGVDIDQDALRQFPFEQMAAAEILATGKPLLLTGHNQDRF
                     AEHRYFESLGVYSAMLCPLLVQGAPYGFLAIGSKRRNAFSERDLALTEQIGFHLSHAL
                     TNLSAYDQIRRLKDQLEQENVYLREEMGASLDLKSLVGDSPALQKSLRAIEMVAPTDS
                     TVLITGETGTGKELVAQAIHRLSPRRQKTLITVNCAALPPTLIESELFGHEKGAFTSA
                     TTRKLGRFELAHGGTIFLDEVGELPIDVQMKLLRVLEAQEFNRVGGSHTIRVDVRVLA
                     ATNVDLEQAIKRGAFRADLFYRLNVFPLRLPTLRERREDIPLLARHFAKKYGLRHRKP
                     VTRINSAALKALSAYDWPGNVRELEHLMERAVIVSRGSVLTIEELDGLGPSHERPSPP
                     RTLAEAERAHIVEVLVRTNWVLAGKQGAAETLGMKRSTLQHRMKKLDISRPPRHVS"
     gene            10483..10899
                     /locus_tag="PLS35_09080"
     CDS             10483..10899
                     /locus_tag="PLS35_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6022 family protein"
                     /protein_id="HNI18856.1"
                     /translation="MKFIVSPLTPESVGREIQAYVNERQRQLLPSGEVTSEGRRFSWC
                     GDILDFLADGLAERLAPLGITFTNPFPGPLRLMDEVQQPDGQHVRRFWTVALHRGCPI
                     ARVCTLFFHRHDQISLPQVPLVMAFSPDHTAGEDGE"
     gene            10896..12026
                     /locus_tag="PLS35_09085"
     CDS             10896..12026
                     /locus_tag="PLS35_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI18857.1"
                     /translation="MNYVALRMLFGDRAKYLMLLCGLTFAVMLIVQQGSIFWGLMMWS
                     QSSISNINVPIWVTDPGIGQVDEVKPIADTAVDRVRSVSGVEWAVPLYKGVLRARLSS
                     GDYHQITLTGLDAPTLIGRPAEVLEGRFEDILEPDAVVLDQWAVERMGGPSVIGIGTI
                     FELNDKLARVVAIAKTQKSFTNIPVVYTTYERAIRYVPRERRTLSYVLAKAKDGIPAS
                     EVTSRIHEQTGLGAFSAEEFGWKTISWVLKNTGIGINFGTTIILGFIVGMAIAGQTFY
                     LFTVENLKQFGALKAMGTSTFTLARMILLQAFTVGLTGYGIGIGLATGFGFLSAKGGG
                     LPFVETWQLLLLVLLALLGICTLSALISITKLARLEPAIVFR"
     gene            12071..12778
                     /locus_tag="PLS35_09090"
     CDS             12071..12778
                     /locus_tag="PLS35_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18858.1"
                     /translation="MASVAVQVRGLVKSFGTGETTVTVLKGIDLDVHFGELLLLVGES
                     GGGKTTLLSAIAGILDIDAGDLQVLGASLTNMTPGTRTSFRGQRMGFVFQQFNLLPAL
                     TAAENVAIPLLIQGLRRPEAVSRGRLMLERVGLGDRTEFLPRNLSGGQQQRVAVARAL
                     VNDPQLLVCDEPTAALDGPNGQKIMELIRDVGRAPNRCVIVVTHDSRVFHFGDRMAEL
                     TDGRIVGIHPIHPEATA"
     gene            12775..13767
                     /locus_tag="PLS35_09095"
     CDS             12775..13767
                     /locus_tag="PLS35_09095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18859.1"
                     /translation="MTILNRVSVWLAVLCTAFAGWSVLTAGNTVPMPTPVVEPPRSPF
                     ETTVAASGIIEAANENVRIGPPSAGLVTKVFVAVGDAVREGDPLLQLDDRDLRAQLVA
                     RQAAIPPAEAQMEEQKYRIGDLDTQLKRLKSVRDSRAVSDDDVKRTWYAAEMAKRAMS
                     RFQAALKQVIAQRDETQLLLDRLTVRAPRAGTILQVNIRAGEFALTTGATEPLMLLGD
                     MQQLQVRAEVDEVNAPLVAPQRPAVAYLKGNTAQTIPLTFVRIEPYIVPKKSLTGDNN
                     ERVDTRVLQIIYRFERPAFPVYAGQQVDVFIERTPAGGPPQTQPVERRGEVSRK"
     gene            13764..15281
                     /locus_tag="PLS35_09100"
     CDS             13764..15281
                     /locus_tag="PLS35_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNI18860.1"
                     /translation="MNRWILASRAATAVLMTLTVSLTGCLQGKAYTRPSVDTPADWTH
                     VASGPLVSGTSPKTPDADWWKAFRNEELTQFVERALARNHDVRRAVSRVIEGRASVTT
                     AGAGLYPQVNVQSSYTNISISKNTLAGLGLATGQQPGPQVFARPGSSFDLWNGAADLR
                     WELDVWGRIRRGMEAASADAQAIEQDARAIALTLIGDVGQAYFRIRELDEQIEIAQRA
                     LALRRDSLEIITKRASVGLASDLDVKRTEVLVAESAGQIPDLVRLRTVEVHRLEVLTG
                     SAPGGLTLASMPLRKVVVQPDIPVGLPAQLLERRPDILQAEATLIAANARIGQARAYF
                     FPTFSITGQGGLQSAEFANWFTGNSANFSIGPSVTLPIFLGGTNVARLDAAESRYQQM
                     LEGYQQTILLAFREVADLLVSIQARAEQLTRQREQAVAATAAVGLAEVRYRKGLVNYL
                     DVLDAQRTMLAAETQLAQTERARLTDMVSLYKALGGGWSQPPGITSVPASSSMTR"
     gene            15472..16368
                     /locus_tag="PLS35_09105"
     CDS             15472..16368
                     /locus_tag="PLS35_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18861.1"
                     /translation="MRDGVRGQRLARPLYPASIILALGLVLPFSTQASSASQETSAAK
                     SGIAGTNLLEEEQILSTTDKITQPIDTDAEAMRHNDLGVAFVFKGDVGQAIDEFTHAL
                     RLQPNYFAAHLNLANTLLDIGRHDAAMIEFKEALRLKPDDPKTHNDLGVALKEMGDPA
                     GAIAEFKTVLRRNPTDVNAHNNLGVALKAMGDLDGAIAEYRTAASLQPNDVNAHFNLG
                     LGLMEKHQPDAAIAEFRTALHLRPNDAKIRFNLAHALAGLGQRMEAAQELRQYLRLEL
                     DTPANRRWLEQAEAKLRELEMP"
     gene            16507..17226
                     /locus_tag="PLS35_09110"
     CDS             16507..17226
                     /locus_tag="PLS35_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18862.1"
                     /translation="MRPHHTMAQFVRLVIAAGSLMTMICVGALWPDRVPGAEEAESDP
                     QHQRHWTFDSSSPGMLPGSYVIGTLFDGRPAGEWKILITDRAKSASQVLAQLQAKGTD
                     QTHKLLLFEGTESANLDVEVSYLAVAGKADFGGGLLWRAKDDRNYYLLRASLVEQKIR
                     VYRVVNGVQQIVKQVDRPLPATGWHKLRIVQRGCEIKALFDDAVLFRVCDTTYTSGRI
                     GLWTKADAVTYFDDLTLKVLD"
     gene            complement(17444..17770)
                     /locus_tag="PLS35_09115"
     CDS             complement(17444..17770)
                     /locus_tag="PLS35_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNI18863.1"
                     /translation="MGSKIYVGGLPYSATEQQLSDLFAVHGAVESARIITDKFTGQSR
                     GFGFVEMASSDEAQKAISALNGTDMGGRTLTVNEARPQEPRSGGPGRSGGGGGFNDRG
                     GKRDRW"
BASE COUNT         3791 a         5532 c         4999 g         3526 t
ORIGIN      
        1 tcgtttgccc tttcaacttc tccgcccacg acgggagttg atgggacatt gctgtttgtt
       61 gaggcaccgc atgatccgcg tgcagctgac tttcagccga cgcggctagc ggcaggcaca
      121 caagcgcgcc gccagcaatg acgctgaggg cgcgcgatgt gacgcgagtg aaccactgac
      181 aatccatgga ccggcctcct tggatagagt ttggatagaa actgatgtaa atgttgagcg
      241 cttacgaact agaacatgac aacctcgata aaaagcgact cgttctggtt gtaatcctgc
      301 ggtttcacgc ccgcagggca ctcccctgag acaagggccg gagattactg aactcacccg
      361 tctctgtcaa cccccttgcc cccgtcgcga gggtgagtgt cacctacgcc ctcagtgatt
      421 cacactcctt ctccgtatca gtctggcacc atgagacatc ggaaggcgac aattgaactt
      481 cggtcgatga ctcagcggga atcgtcacgc tccattgtct cttttccgcc agttgaggca
      541 taatgtcgca tgatcttgaa acgctggctt gttggcctgc cactcaagac gaaggaagcc
      601 gctcacgaac gcctttctaa gcgactcgcc ctagccgtct tttcctctga tgccttatct
      661 tcagtggcct acgccacgga agagattctg ctcgtcctca ccctggccgg gacggccatg
      721 gtcggctatt ccatcccgct cagcctttcg attatcggtc tgctggtaat tctgaccatg
      781 tcgtaccgcc agatcatctt tgaatatccg gagggtggcg gcgcctacat cgtcggcaag
      841 tccaaccttg gagaatggcc cggcctcgtt gcggccggcg cgctcatgat tgactatgtg
      901 ttgacggtcg cagtcagcgt cgcggccggc atcgcggcgc tcacgtctgc cttccccgac
      961 ctgctcgcgc atagggaggc cctctgcgtg gcggcgatcc tcctggtcac cgtcgtcaat
     1021 ctgcgcggcg tccgtgagtc cggacagttt tttgccgtcc caacttatat ttttatcggc
     1081 acactcacag ccatgctcgg cgtgggcgct attcaaatcc tgctcggcca cgcgaccagg
     1141 atcgaaccgc tgccaagcct gactccggcc gaaccattga cgctgtttct cctcctgcgc
     1201 gccttttcgt ccggctgtac ggcgctgacc ggcgttgaag tgatttcgaa cggcgtctcc
     1261 gcgtttaaga aaccggaggc caaaaacgcc gccttcacca tgatcggtat ggccgtgatt
     1321 ctcggaaccc tgttcatcgg catcagcgcc atggcctact acttcggcat tgtgccgaaa
     1381 agcgacgaga ccgtcgtctc tcagatcgcg cgcgccactt tcggaaccgg cctgctatat
     1441 ttcctcgtcc aggcctcaac catgaccatt ttgatcctgg ctgccaacag cagcttcaac
     1501 ggatttcccc gtctggcctc gattctcgct cgtgacagct acatgccgca tcaaatgtcc
     1561 atgatgggcg atcggctcgt gttctccaac ggcgtgatca ttcttggcgt gttttcctgc
     1621 ctgctgatca ttctcttcaa cggcgacacc catgctctca ttccgctgta cgccgtcggc
     1681 gtgttcctgt ccttcacgat ctcacaggcc gggatggtca aacgctggct cgtcaagaag
     1741 ggaccgcact gggaaaagaa gctgctggtc aatggtatcg gtgccgtcac gaccgccatt
     1801 gcaaccgtga tcattgccag caccaagttc gcccatggcg catggatcgt catcgtgctg
     1861 atcccgatgt tgatcatgtt tttcaaggcc attcgctccc actataaagc cgtttcggaa
     1921 caggtggctc tatcccgagg ccaccgcccc cctctgcccc ggcgcaatat cgtggtgctc
     1981 ccgatcggcg gcgtcaatcg cgccgtcatc cgcgcagtag actatgcgcg aagccggtcc
     2041 ggagacattc gggctgtcct ggtggatgtc gatcctgaag aaaccgcgcg ggtggaaatc
     2101 caatgggccc agtggggttg cggtgttccc ctcacggtgc tgccgtcccc ctatcggtcc
     2161 gtcctgagtt ccctattgga ttacctcgaa caagtactgc aaaaggacca ggaatgctgg
     2221 gtgaccgtcg tgatcccgga aattcttcct gcccggtggt ggcaaaatat cctgcacaat
     2281 cagcgggcct tcatgctcaa aggggccctc ttgtttaaag atcgcgtgat cctgactgat
     2341 gtgccttatc acctgacgag gtgagcatgc gaaatccacg agccacaatc catcgtacaa
     2401 accggcgagt aggcgcccac ctggtcgcca tggtcctcct gctgggactc tctccccatc
     2461 cggggttggc cttcacgatc gtcgctccta aaaacggcac aacgttgacg tccggcgagc
     2521 caaccaccgc cgaagtcgaa gcagggcggg aggcgggcct cgtcgaagtg cgctactact
     2581 ggtatccgga gcagacggaa tctctcgtgg agcaaggcga cgagcggtct ggaaaaggct
     2641 cccagggcag catctcaaca gaaaagtact ggcagaaaga cagcatcaca ggagcgcccg
     2701 tggtcgcgct gccggcgctg acttcaacgg tggaccgggt gccgccctat ggtggcgcat
     2761 tgcctgttcc gcctgacgcc gtcggccgga tgcggctcct ggccatcgcg gatatttcac
     2821 aaggccgcct gggacgcaaa accgtcttcg atgaagtctt cgtcaccgtt cagccaaagg
     2881 ccgagcttct ctcgatcgat tttgaaacgg aaaaaccgct ccaacttggt cgaacaggac
     2941 agtcctccgc ctatggccat gtcgattcac tgggcaagat cttcgaactc ccggtggtag
     3001 gcgaatttgc agacggcatc agccgcccgc tgtcctctcc cagcgccggc accgtctata
     3061 catccgggaa cgatcagatc ctcaaggtcc tgcccgatgg attgttgcag attgtcggca
     3121 tcggcaagac ctcgctcacc gtcaccaatc gcggcaagca ggccacactc gacgtacgcg
     3181 tggaggtcaa tccggagccg aatgaaccac ccattgccaa tgccggtacc gccaaaacag
     3241 tgaaggccgg cacgaaggtg cgcttgagcg gattgcaaag tcgcgacccg gagggagaag
     3301 cgctgtttta tgcgtgggca caggtgcgcg ggagtaaagt ggccatgctg gacgtgaata
     3361 tggcggaacc gtcgtttacc gccccgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnct ctgtatctgc ggggatacgc tcgtatcttc tccgatacag accttgcagc
     3541 cttccaaccc ctccatcata aagaccgcat acagtgcgct gcatgtctgc cggaacggaa
     3601 gtgacgttcg cgcgatgccc ttgtgctagt atcaccctca tctagttgaa taccgtctcc
     3661 taccgccaac catggagggt tcctcatggc actcattcaa caggacctcg ttgcaggcag
     3721 cgaggatcgg ctgctcacgc tggataccga gacgaatcta cagtggctga atctttcgca
     3781 gaccgccggg cgatcgtatc aagagattat gaacggattc ggccagttca cgacggcgca
     3841 cgggtttcac tatgccgacg gtcgtcagat cggcgagtta tgcgggcatg ccggcattac
     3901 gaaaggcttg acggaaccag ccctcacgcc ttcccctaat gatgcccgga atcaccaggc
     3961 gatccagacc ctccaaaacc ttatggacgg caaagtgttt catgccgaga ccaacgtgat
     4021 caccagccgc ggcatcatga aacctccggc tccgccaccg aacgtcccga cgagaattct
     4081 gggaaccatt cgactgtctc tcagcctctt gaatatcacg ggctcccatg cggaaagcga
     4141 aggaccgacc gcaagcccgc agaccggcga tccggaaatc ggttcttatc tggttcggaa
     4201 tcagccggag gcgtgagcgc accaacaacg ggagacgtgt ggtctgacaa caagacggca
     4261 cgacgcgatt catgcgcaca gtacacgggc cccggcgtga actgacgccg gggcccgttc
     4321 tgcagccacc tcactcccga gtccatgagg ggattacatc aggctcaacg tctctcgcgc
     4381 ctgagccaat tcgattcgat gacggcgcag cgcgggacca aacgcggatg cactgacgcg
     4441 ttgctcgtac tcctggattc ctcgttccat agaggtaagc gcatcgcgta attgcgttac
     4501 cagtcgcttg ccgccccttg taagccactt cagatgcttg cccgcatact cgagatcctg
     4561 cagcgaataa cggactgatt cgatttcttc caggcaacgg taccgagcac gggcgaggtc
     4621 gcgttgggtg aggcccgcct tcgtccagcc ggcttttcct gacgtacctg atccccatac
     4681 cacgagccgc tcgaacagca tggcgcccat cgtaaaggtg aaggtcgcat gcaggattcc
     4741 gcgcaacggg cgcagtgtgc gccgccaggg cgaatagaaa acttcctgat tgtgatcgtg
     4801 ctgatacatc acatcgttgc gcagcagcag attgaggtga tgatgactat tctcgtggat
     4861 caaatcatcg atcaggtcca gttgatcgcg ctcaaagcaa ttgatggcgg agaaccccgg
     4921 acgatgccgg taactgaagc tgacgacacc cgcggctttc aacggcacga tgcgcgaggt
     4981 cagcaacgcc agcaaccggt ctccttccgg ccaggccgcg gcaatggccg ataacgcccg
     5041 gcgcatgcgc acggcgactt cctgtttggt cggtcgcaca ccgatcggcg tcttgtcttt
     5101 tccatacacg agtgtcggcc caaggacgag tgatccgtac cggctgtccc gaagcggcaa
     5161 cgggtcgagg cgacgccaat gccaccgctc ctgactgcca cacgtcatca ggggataggc
     5221 acgatcgccg accaacagct cgatccctga ggccgtgatc agaaattccc cttggccttt
     5281 cgcgcgctgc agtgctgagc ggaccgcacc agtcgcctca tagaacacac cggtcgtccc
     5341 gattgcacag ccccagggca attccactcg ttcgacattt gccgtaagat cacaaggcag
     5401 cagccaggcg tcccgccgtt gcttcgccac ccatgcacag gcaagccggg gagccagaca
     5461 cacggactct tgcgtgattt cccgagccag ccggtgacag agcgccgtta accgcccaag
     5521 aaggagtcgc ggctccggtt tgccgttggg aaaaatctca tcggtatacc cgtgctcgta
     5581 gaatttctcc ctaaactccg cgcgcaactg ttcggcaata tccctccgcg accgttcctg
     5641 ccggacctgg gtcacgatat cgacgaaata cagcaagtca ttcagcgact cgatccatcc
     5701 caccactttc cagttgctaa actcggctgg cgggaaagac cgtccgagcg acaggaacca
     5761 gtcgatcggt aattgaaacg tgctgactgc gtcggcataa tttttcgtga aatcgcggca
     5821 cacctccgtc aacaaccctt gcatggatcg ccggaattct tccatcaacc gctcaagcat
     5881 gcgcgcatca agaaccgtca tcgctgatcg cctttctgaa tcgaagagag ccgggcgcac
     5941 tctacgcgac tgcttgatcg ctgacaagca tgcaacatga ctgcaagcag tgaacaccca
     6001 tgacacattg gggagaattg cgcgggtgga ggggaagcag cagtgaagat atggagacgc
     6061 agggcgccgt gggatacgcc cacgcgcgtc atgcgtggag aacagccccg cgaaaccgga
     6121 gcggcttcgc ggggaaacgc gttacgccga gcgcctgatc aactcaaaaa gaaccggctt
     6181 ctcggggaca gaggcaggga ccgggcggag atgggagttc tgcaccatcc ggtgtcgttg
     6241 aatcaacgta tccaccacat taccgcagct gaggcagcgg tgcccacgca accgcatggg
     6301 atgataactt tcctggagat cgaccaaatc gtccatcacc attaggcctt cacatcgcgt
     6361 gcatgtcatg agtcgcctcc cttcgaacag cctctgagat ccccgtcaag acgggacgat
     6421 ctgttgccca ttcgtacaac tcaccaacct ggtgctatgg agagaagcaa tgtggatgcc
     6481 accgactctg gggtgcagag gacgatttgg ccggatgagg cccctgctct caaacgtgag
     6541 cggctcgtgg gaattgagct aattgtgacc gaattggtct catgggacaa aaatgggtag
     6601 aatcggctac acaaccggca acgttccctc ctccggtacg gttcagagaa attgaccggg
     6661 caaatcagga atggccgaag cggcatcgcg gtcaaaaaat ggctcttact gcgacagaaa
     6721 ttggttccat tgtccaggaa ctggcatcag cgctcgccga cgggtggatt cagaagattt
     6781 cccaaccgct tcccgacacc cttatcctcg agatccgtgt ccccggacat actcgccgcc
     6841 tgctctactc actgcgggat gacacggcgc ggctgcatct cgtccatcag catctaccca
     6901 acccgccgac cccaccttcg ttctgccagc tgctccgggc tcggatccaa ggcgcccgca
     6961 tcgacaccct tcaacacatg tccggcgatc ggatcgttcg acttgatctc accagccggg
     7021 acggacccgc ctctctcgtc gcagaacttt tcagccgcaa tgcagacctc ctctttctcg
     7081 atggcgaagg cctggtgctc gctacgcttc gtcacaataa agaccgcgta ggtcaaccct
     7141 atcaggcgcc ggcggctgca ccgtttcgat cgtcatccca tcaggcggtc gttcacgagc
     7201 cggatacgca gccgcagacg gacgtggatc catttccgct gtcatccaga ctcgaggcgt
     7261 tctatcgaga gcgcgaagcc gaattgtcgc accaggctca ggcccgcgaa cgggaatcgg
     7321 gactgcgaaa acatctcaaa aagctcctgc gccgcatggc ggctctccgc caagacctcg
     7381 aacaggccgg acgatatgaa ccctacgcac ggtacggcga attgttgaag gccaatctcg
     7441 gcttactgaa gaagggccag aacagggtgg cggtcgtcga ctactacgat gaacaattgc
     7501 cagaactgac gattccgctc gatccgacca aagggccgca ggccaatatg gacgcgtact
     7561 ttgccaagta ccggaagttc gtgaccgcgc aacgcgaaat ctcgccccgc atagctgcga
     7621 tcgaagcaga ggttcaacag atcctaaccg aattggagac catcaaacag ggaacctggc
     7681 agccaccgag cacggaagga caaaccgcgg cacgaggaac ccctgcccta cgacggaagc
     7741 gcgacaggga gggggaatcc cggggaccgt tccgccgttt tgtctcatcg gacggccatc
     7801 ccatcctggt cggccgcaac gcccgggaga atgatgagct caccttcgga ctggcgaaga
     7861 gcgaagacct ctggcttcac gcgcgcggta cgcccggctc gcatgtggtc gttcggctgg
     7921 acaaggggac cgagccccct cccgaaacca tacgagatgc ggccacactg gccctcctct
     7981 acagcgatct gaagaagagc gggaaaggtg acgtgatcta tacccggcgc aagtgggtca
     8041 aaaaggccaa ggggcaagcg gcaggcgccg tgaccgtgac gcaggaaaaa tctatctatg
     8101 tcacactcga taaaccgcgc ctggatgccc tgaaatccag aagcgcaccg cgatgacgca
     8161 gattcggcca tcctgaatcc ttcatttcac cctgagcgat acaattcctg tccgtccctc
     8221 tccgtcttcc catcactcac cacgacatgg tagagtgccc acatatgggc accgcgttcg
     8281 acacacgtga ctggtttccc attctaagcg cgctccttca aaccaacacc ctccgccagt
     8341 tcgacgatcg actccaacgg gaactcagcc aacgcctagg ctgtagcgtc atcggcctct
     8401 atctctacag tgagatggcg accgcgtttt cacccgtctc ggaaagcttg cgtgatccgg
     8461 cgtcgcccgc ctatcccatc gcgcaattac ccgcgtccgg gaccatcaag gaagcagcgg
     8521 tcctggccgg ggaagcgatc ctgaccggcg atctcagcac gtcaccctgg acggaagccc
     8581 gagccatagc acctcatatc agccatggca catcggtcct cgtctcacca attagaatgt
     8641 catgcgaatc ggtgaccggt acagcagcca agacgctggg cgtcatcgcc gcggtcgcac
     8701 ccggtaaacc gggggcgttt tccgaggagg atcggatatg gctcgaccag ttgagccggc
     8761 acatcgcccc ggttctgacc gccgtgctcg ccgcggaaga acgggacacg ctggtcgcca
     8821 tcaacaatca agtggtgctc ggcactctca cgctcgacaa tctgatccac tcgattcaac
     8881 caatcctccg cgaagtcatc catcacgaca tcaccgggct cgtacggttt gtcggcgaac
     8941 cccacaatcg gtggttcgac atcgtggcgt gcgagggagt cgatatcgac caggacgccc
     9001 tgcggcaatt tccgttcgag cagatggccg ctgccgaaat cctcgccacc ggcaaacccc
     9061 tgctgctcac cggacacaac caggaccgat ttgccgaaca ccggtacttc gaatcactcg
     9121 gggtctactc cgccatgctc tgcccgctcc tggtccaggg agcgccctat ggtttcctgg
     9181 ccatcggaag caaacggcgc aacgcatttt ccgaacgcga cctggcgttg acggaacaaa
     9241 tcggctttca cctgtcccat gccctcacca acctctccgc ctatgaccag atccgacggc
     9301 tcaaggatca actggagcag gagaatgtgt acctgcgtga agaaatgggc gcctccctgg
     9361 atctgaaaag cctcgtgggg gatagccccg cacttcagaa atcgcttcga gccatcgaaa
     9421 tggtcgcgcc cacggattcc acggtgctga ttacgggaga aaccggcacc ggcaaggaac
     9481 tggtggctca ggccattcac cggttgtccc cgcgccggca gaaaaccctg atcacagtga
     9541 actgcgcggc actgccgccc acgctcatcg aatccgaact gttcgggcac gaaaagggtg
     9601 ccttcaccag tgcaacgacc cgcaagctgg gccggttcga actggctcat ggcggaacaa
     9661 tcttcctcga tgaagtcgga gagctgccca ttgatgtcca gatgaaactc ttgcgtgtcc
     9721 tcgaggcgca ggagttcaac cgggttggcg gaagccacac catccgtgta gacgtgcgtg
     9781 tgctcgcggc aacgaacgtc gatctggagc aagccatcaa acgcggcgca ttccgtgccg
     9841 atctctttta tcgtctgaac gtcttccctc ttcgcctgcc gacgctgcgg gaacggcgcg
     9901 aagacatccc gctgctggct cgtcattttg ccaaaaaata cggccttcga catcgtaaac
     9961 cggtgacgcg catcaacagc gcggcattga aagccttatc cgcctatgac tggccgggaa
    10021 atgtccggga actcgagcat ctcatggaac gggcagtgat cgtcagccgg ggctccgtgc
    10081 tgaccatcga agagctggac ggtctgggtc cgagccatga gcggccttcc ccgccgcgca
    10141 ctttggccga agccgagcgg gcacacatcg tagaggtgct ggttcgaacg aattgggttc
    10201 tggcgggaaa gcagggagcc gctgaaacat taggcatgaa gcgatccacc ttgcaacatc
    10261 gcatgaaaaa attagacatt agtcgccctc cgaggcatgt ctcgtaactg cccgcatatg
    10321 ggcaccgctc cttagatctg ccaacatctc ggcagatacc attagagatt cactccaccc
    10381 cacatcctct cctcttcagt attccagcaa attttcatca cgatatgatg ttctggggcc
    10441 accacccttc atccggcatg gcgcatgctt tctgtcgatc ccatgaaatt catcgtgtca
    10501 ccgctgacac cagagtctgt cggtcgtgag attcaggcct atgtcaacga acggcagcgt
    10561 caattgcttc cctccggcga ggtcaccagc gaggggcgcc gtttctcctg gtgcggagac
    10621 attctggact ttctggcaga tggactcgcc gagcgattgg ccccattggg aattactttc
    10681 accaatccat ttccagggcc cttgcgcctg atggatgaag tgcagcaacc cgatggccaa
    10741 catgtacgga gattctggac cgtcgccctg catcggggct gccccattgc cagagtctgc
    10801 acgttgttct tccatcgaca cgatcagatc agccttcctc aggtgccgct cgtgatggcc
    10861 ttttcgcctg accacaccgc cggggaggac ggcgaatgaa ttacgtggcg ctgagaatgc
    10921 tctttggcga tcgcgcgaag tatctcatgc tgctctgcgg gctgaccttc gcagtcatgc
    10981 tgatcgtgca acagggatcg attttctggg gcctcatgat gtggtcgcag tcgagcatca
    11041 gcaatatcaa tgtgccgatc tgggtcaccg accctggcat cggacaggtc gacgaagtca
    11101 aacccattgc ggataccgca gtggatcggg tgcgtagcgt atcaggtgtg gagtgggccg
    11161 ttcccctcta taaaggcgtg ttacgcgctc gcctctccag cggggattat caccagatca
    11221 cgctgaccgg actggacgcc cccacgctca tcgggcgtcc cgccgaagtg ctggaaggac
    11281 ggtttgaaga tattctggaa ccggatgccg tcgtcctgga tcaatgggcg gttgaacgta
    11341 tgggcgggcc ctcggtcatc ggcatcggga cgatttttga gttgaatgac aaactggccc
    11401 gggtggttgc gattgcgaaa acgcagaaaa gcttcacgaa catccccgtg gtctatacca
    11461 cctacgaacg cgcgattcgc tatgtcccgc gtgagcgccg gacgctgtcg tacgtgctcg
    11521 ccaaggcaaa agacggcata cctgcctcag aggtcacctc caggatccat gaacaaaccg
    11581 gactgggagc gttcagcgcc gaagagttcg gctggaagac catcagctgg gttctcaaaa
    11641 ataccgggat cggcatcaac ttcggcacca cgattattct cggctttatc gtcggcatgg
    11701 cgatcgcggg acagacattt tatctgttta ccgtcgaaaa cctgaaacag ttcggtgcgc
    11761 tcaaagcgat gggaacctcc accttcacgc tcgcacggat gattctgctg caagccttta
    11821 cggttggact gacgggttat ggaatcggca tcggccttgc cacgggattc ggatttctct
    11881 cagccaaggg cggcggattg cccttcgttg aaacctggca gttgctgctg ctggtactcc
    11941 tggcgctctt gggcatttgc accctgtcag ccttgatcag cattactaaa ctggcccggc
    12001 ttgaaccggc gatcgtgttt cgatgatgtc gatggatggt ccaggacaca gcgtagcgca
    12061 ggaacagggc atggcttccg tggcagtgca ggtgcgaggc ctggtgaagt cgtttggaac
    12121 cggcgagacc accgtgacag tgctaaaggg gatcgacctc gatgtgcact tcggcgaatt
    12181 actcctcttg gtcggtgagt ccggcggagg caagaccaca ttgctgtcgg ctattgccgg
    12241 gattctcgat atcgacgccg gcgatctcca ggtgctgggg gcgtcgctca caaacatgac
    12301 gcccggcacg cgaaccagtt ttcggggaca gcgcatggga tttgtctttc aacaattcaa
    12361 tctcctgccc gcgctgacgg cggcagagaa cgtcgccatt ccgctgttga tccagggcct
    12421 gcggagaccc gaagccgttt cgcgcgggcg actgatgttg gaacgcgtgg ggctcggcga
    12481 tcgtactgaa tttctgcctc gaaatctctc ggggggacaa caacaacgtg tggccgtcgc
    12541 gcgcgccttg gtcaatgatc cccaactcct ggtctgcgac gaaccgacgg ccgccctcga
    12601 cggacccaac ggacaaaaga tcatggaatt gattcgcgac gtagggcgag cgccaaaccg
    12661 ctgcgtcatc gtcgtgaccc acgacagccg ggtcttccac ttcggtgatc gcatggcgga
    12721 actcacggat ggccgtatcg tgggcattca tccgattcac ccagaggcaa cggcatgacg
    12781 attctcaatc gtgtcagtgt ctggctcgca gttctctgca ccgcatttgc cggctggtct
    12841 gtactcaccg cgggcaatac cgttcccatg ccgacaccgg tcgtggagcc accccgctct
    12901 ccatttgaaa ccaccgtcgc ggcaagcggc attatcgagg cggccaatga gaatgtgcgt
    12961 atcggcccgc cgtctgccgg gctcgtgacc aaggtctttg tcgcagtcgg ggatgcggtg
    13021 cgcgagggcg atccgctctt gcagttagac gatcgcgatc ttcgcgctca gttggtggcc
    13081 aggcaggcag ccatcccgcc ggcagaggcg caaatggaag agcagaagta ccgcatcggc
    13141 gatctcgaca cgcagctcaa acggttgaaa tccgtacggg atagccgggc ggtcagcgac
    13201 gacgatgtca aacgtacctg gtatgccgcg gaaatggcca aacgcgccat gagtcggttt
    13261 caggcggcgc tgaaacaggt cattgcacaa cgggatgaaa cccagttgct gctggatcgg
    13321 ctgacggttc gcgcgccccg cgccggcacg atcctgcagg tgaatatccg ggcgggagag
    13381 tttgcgctga cgacaggtgc gaccgagcca ctcatgctct tgggcgacat gcaacaattg
    13441 caggtccgtg cggaagtgga tgaagtcaat gccccgctcg tcgccccgca gcggccggcc
    13501 gtcgcctact tgaagggcaa cacggctcaa accattccgt tgaccttcgt gcgcatcgaa
    13561 ccgtacatcg tcccgaaaaa atccctgacc ggtgacaaca acgaacgggt agacacccgc
    13621 gtcctccaga ttatctatcg ctttgaacgg cccgcgttcc ctgtgtatgc gggacagcaa
    13681 gttgatgtgt tcatcgaacg gactccagcc ggtgggccgc cccaaacaca gcccgttgaa
    13741 cgtcgcggtg aggtgtcgcg gaaatgaatc ggtggattct tgcatctcgg gcggccaccg
    13801 cagtcctcat gacactgacc gtgtcgttaa ccggctgcct gcaagggaag gcctatacac
    13861 gtccgtcggt tgacacgccc gccgactgga ctcatgtcgc ctccggccct ctcgtctcag
    13921 gcacctcccc aaaaactcct gacgccgatt ggtggaaagc atttcgaaac gaggaactca
    13981 cgcaattcgt cgagcgcgcc ctcgcgcgca atcacgatgt gcgtcgggcg gtctcccgtg
    14041 tgatcgaggg acgcgcatca gtgaccacgg ccggagcagg actctacccg caagtgaacg
    14101 tgcagagcag ttacaccaac atctccatct cgaaaaatac cttggcggga cttggattag
    14161 ccaccggcca gcaaccgggc ccgcaggtct tcgccagacc gggcagcagt tttgaccttt
    14221 ggaacggagc cgccgatctg cgatgggaac tcgatgtgtg gggtcggatc cgtcgcggaa
    14281 tggaagctgc ctcggccgat gcacaggcga tcgaacagga tgcgcgggcg atcgccctga
    14341 ccttgatcgg cgatgtgggg caagcctatt ttcggattcg cgagttggat gagcagatcg
    14401 aaatcgcgca acgcgccctg gctttgcggc gagactccct ggagatcatc accaaacggg
    14461 catccgtggg attggcctcc gatcttgatg tgaaacgaac ggaagtcctg gtcgccgaaa
    14521 gcgcgggaca aattcccgat ttggttcgct tgcgtacggt ggaagtccat cggctggaag
    14581 tgctgaccgg atcagcaccc ggcggcctga ctctcgcatc gatgccgctg agaaaagtgg
    14641 tcgtccagcc cgacattcca gtcggcctgc ccgcacagtt gctggaacgg cggccggaca
    14701 tcctgcaggc cgaggcgact ctcatcgccg cgaatgcccg tatcggccag gcacgggcct
    14761 atttctttcc caccttctcc atcacgggcc aaggaggcct gcagagcgcc gagtttgcca
    14821 attggttcac cggcaacagc gccaacttca gcatcggccc atccgtcacc ttgcccattt
    14881 ttctcggggg gaccaatgtc gcacggctgg atgcggcgga atcccgctat caacaaatgt
    14941 tggaaggcta ccaacagacg attctgctcg cctttcgaga agtcgccgat ctcctcgtgt
    15001 cgatccaagc ccgcgccgag caactgacac gccagcgtga gcaggcggtc gccgcgacgg
    15061 ccgccgttgg cctggcggaa gtgcgttatc gaaaggggct ggtgaattat ctcgatgtcc
    15121 tggatgcgca gcggacgatg ctggcggcgg agacgcaact cgcacaaacg gagcgggcgc
    15181 gcctcaccga tatggtgagc ctctataaag cgttgggcgg cggatggtcg caacctcccg
    15241 gaatcacttc tgtcccggca tcgagttcga tgacacggtg accctttctc ccccgtccct
    15301 actcatatca tcagccgggc accgcgacgc cgcggacttt caacttgaat tttccggcca
    15361 gatcgggcta tctgacgcag tgcaatccct gacccccgat caagcggcag gccagacacc
    15421 ggccagcctt ttcacgctaa tgcctctctg acttctcgga aggagcctcc catgcgagac
    15481 ggtgtccgcg gccagcgttt ggcacgcccc ctgtatcctg cttcgatcat tttggcattg
    15541 gggcttgtcc tcccattcag cacacaggcc tcttccgcct cgcaggaaac ctctgcggca
    15601 aaatccggca tcgccgggac aaaccttttg gaggaggagc agattctctc gaccaccgat
    15661 aagatcacac aaccgatcga tactgacgcg gaggccatgc ggcacaatga tctcggcgtc
    15721 gccttcgtgt tcaaagggga tgtgggccag gccattgacg aattcacaca tgccctccgg
    15781 ctccaaccga actattttgc cgcgcatctg aatctggcca acacactact tgatatcggt
    15841 cggcatgatg ccgccatgat tgaattcaaa gaagccttgc ggctgaagcc ggatgacccc
    15901 aagacgcaca atgatctggg ggtggcactc aaggaaatgg gcgatccggc gggggccatc
    15961 gcggaattca aaaccgtgtt acgccgtaat cccaccgatg tgaatgccca caacaacctg
    16021 ggggtggcgc tcaaggccat gggtgatctg gatggggcga ttgcagaata tcgcactgcg
    16081 gcgagcttgc aaccgaacga tgtgaacgcc cactttaacc tggggctcgg gctgatggag
    16141 aagcatcaac cggatgcagc catcgccgaa ttccgtacgg cactgcacct ccgccccaac
    16201 gatgccaaga tccgcttcaa ccttgcgcac gcactcgccg gtcttgggca acgcatggag
    16261 gccgcccagg aactccggca ataccttcgg ctcgaactcg acacgccggc caatcgtcga
    16321 tggttggaac aggccgaagc caaactacgc gaactggaaa tgccgtaaaa aggcggagca
    16381 gtcctggacc tcccgcacta cgtacaggtc gatcatcacc aacaagataa tttttcgaca
    16441 tccctctcgc cgacagcgcc ctgtttaggg tacagtccga aacgccttaa ctgaacaggg
    16501 actctgatgc gcccgcacca caccatggcc cagtttgttc gtcttgtgat tgctgcaggc
    16561 tcgctcatga cgatgatctg cgtcggcgct ctatggcccg acagagtgcc gggagcggag
    16621 gaagcagaga gcgatccaca gcaccagcgg cattggacgt ttgacagttc ttccccggga
    16681 atgctgcccg gttcatacgt catcggaact ctgttcgatg gccgaccggc aggagaatgg
    16741 aaaatcctca tcaccgaccg cgcaaagagc gcctcgcaag tgctcgccca actacaggcc
    16801 aaggggacgg accagacgca caaactcctc ctgttcgagg gcaccgagag tgccaacctc
    16861 gatgtcgagg tgtcctatct cgccgtggcc ggaaaagccg acttcggcgg cgggctcttg
    16921 tggcgggcga aggacgacag aaattactat ctgttgagag cgagtctggt ggagcaaaaa
    16981 atccgtgtct atcgcgtcgt gaatggggtg cagcagattg tgaagcaggt cgatcgcccc
    17041 ctccccgcta ccggatggca caaacttcgt attgtgcaac gcggctgcga aataaaagcg
    17101 ctcttcgatg acgccgttct gtttcgtgtc tgcgacacga cgtacaccag cggacggatc
    17161 ggactgtgga ccaaagccga cgcggtcacc tattttgacg atctcacgct caaggtgctc
    17221 gactaacgta taaccttccc cgcaccctcc ccgcatacat agcaggagtc gcgtagcgtt
    17281 tccgtcgcga atcgcgcctt gtcatcttcc gcgtttcacg gcgtacagaa aaccaccatc
    17341 cccaattggc gcgtacgaga aatccctcac ggctcatccg gacaaaaaaa tggggcagcc
    17401 accacaagga tgactgcccc acgagctgtt acaaagtcag gcgttaccag cgatcgcgct
    17461 tgccaccacg atcgttgaat cccccgcctc caccgctccg gccaggcccg cctgaacgag
    17521 gctcctgcgg acgcgcttca ttgaccgtca atgtccggcc acccatgtcc gtcccattga
    17581 gagcggaaat cgccttctgc gcctcgtctg aagaggccat ttcgacgaat ccgaaacctc
    17641 tcgattggcc ggtaaatttg tccgtaatga tacgggccga ttcaaccgcc ccgtgcactg
    17701 cgaacaaatc gctcaactgc tgttcggttg ccgaatacgg caacccgcca acataaattt
    17761 tagaacccat gtgggtcctc ctcttgggta gtgacattgt cttgaacgaa ggaaagaaag
    17821 ggaaaggacg ggccgaagac gcgacgtaac ggggcgactt gggtctggct ttcgattaga
    17881 ttcccgggca agagcaacat cgagtcaggc ctgaactatt tcaacgcatc cctcaccagg
    17941 ccccagcg
//
LOCUS       DAOQEW010000067        17563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_843_length_17563_cov_7.807859, whole genome shotgun sequence.
ACCESSION   DAOQEW010000067 DAOQEW010000000
VERSION     DAOQEW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17563
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_17563_cov_7.807859"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1037
                     /gene="kdsA"
                     /locus_tag="PLS35_09120"
     CDS             204..1037
                     /gene="kdsA"
                     /locus_tag="PLS35_09120"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNI18864.1"
                     /translation="MAYEVDLGQFKIGVGSRPFLIAGPCVIESEQLVMDTAGRIAEIT
                     KSLGMPYVFKSSFDKANRTSISSFRGPGLEKGLAVLAKVKQQIGVPILTDVHSEEQAT
                     EAGHVVDMLQIPAFLCRQTDLLIAAAKTGKVVNIKKGQFLSPPEMGNAVKKVEDAGNR
                     RIVLTERGSSFGYNNLVVDMRSFPIMRRFGYPVVFDATHSVQLPGGGGTKSSGQREFV
                     EPLACAAAGAGCDGFFMEVHPDPDSALSDGPNMVPLHALHSLLERVLRICDAAAPQPV
                     R"
     gene            1004..2041
                     /locus_tag="PLS35_09125"
     CDS             1004..2041
                     /locus_tag="PLS35_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNI18865.1"
                     /translation="MRRSRSTTSPVTRKTKTSAGRSAAVDASVREGIRVLDIEARAVL
                     DLKARLDERFARAVTLLYECQGKVVISGMGKSGLIGQKIAATLASTGTPSFFLHPAEG
                     VHGDLGMLARRDVLIAISNSGETQEVLQLLPFVKRMNVPVVGMTGKMTSTLSKNSDVT
                     LDVSVDEEACPLGLAPTASTTATLAMGDALAVALLQKRGFKHDDFAQFHPGGSLGRRL
                     LVKVRDLMQHGDHLPRVRADVSGADTILEMTSKKLGLTTVVDAKGSLYGIVTDGDLRR
                     FIQAGGDFSRVTAGDLASRHPKTIGPDELATTAVAQMERFSITALVVIEPPNRLAGVI
                     HLHDLLKHGIV"
     gene            2084..2698
                     /gene="pgsA"
                     /locus_tag="PLS35_09130"
     CDS             2084..2698
                     /gene="pgsA"
                     /locus_tag="PLS35_09130"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNI18866.1"
                     /translation="MGENWKEAGLVLMRSAGQESNINLPNVLTLVRILLIPVFVMLLI
                     DPTPDRALSAAIVFVVAAVTDLLDGYVARKTGQITKLGRLLDPIADKLLVLSALILLV
                     QVDRVSALVAILIIAREVAVTGLRAIAASEGLIMSAEVTGKYKMALQVIAIVLLVLEG
                     TVVETIGNLHLAGIVTLYLSLILGYVSGAQYVWSFWRQVGAKGL"
     gene            2728..3330
                     /gene="plsY"
                     /locus_tag="PLS35_09135"
     CDS             2728..3330
                     /gene="plsY"
                     /locus_tag="PLS35_09135"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNI18867.1"
                     /translation="MDSPWLIGVLIIFAYLLGSVPFGVVVSRLLGAVDPRTAGSKNVG
                     FTNVLRVSGKKAGVLTLAGDIGKGWLAGWVATMVLQQETAILGVALASILGHLHSIFL
                     GLKGGKGVATALGAALGVAPWVGLTLMTLWIGAVFLWKYSSGGALFAFGMFPIVALIF
                     HRSWTFIGFACVVSVLIWMRHKDNLIRLWSGTESQIGQSS"
     gene            3360..3998
                     /locus_tag="PLS35_09140"
     CDS             3360..3998
                     /locus_tag="PLS35_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNI18868.1"
                     /translation="MLSFELLARIRKELFLTGSALYETIVAIAERVNRKVHVLRLHGQ
                     ATTILNQTHAIHRQIGQRVAALAAKPAAGIHPVAPSELASLQDAIRTASDRIKGQQAA
                     LQLVERQIRELKVELAHEDLLTMQRELGLRDAAIERVVVARGAPVIGHPIGEFDLPPT
                     TRIAAVFRGPFLLPLSHTMTLRPDDIVILVGLRHDLAHWLPHFQHPAASKSA"
     gene            4088..5491
                     /locus_tag="PLS35_09145"
     CDS             4088..5491
                     /locus_tag="PLS35_09145"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNI18869.1"
                     /translation="MKTPLSCSTLLCLGLILTGSFLGSPLSAVAAADVASTPRTVSPG
                     LRLLEELQTVITDLAEQAKPSVVSIFPIQATGRSRDGGERVPSSTGSGSGVIVDAAGH
                     IITNNHVVGDAAEVEVRLSDKTKLFAQVIGKDPDTDLAVLKVTADHPLPAAHFGDSSG
                     VKVGQWVLAVGNPFGLDRTVTLGVVSGIGRENINLSRYENFIQTDASINPGNSGGPLF
                     DLRGDIIGINTAIINFAQGIGFAIPSNMAKQVMNQLIAKGKVVRAWLGVGLQPLTPEL
                     ASKFGVDENEGVLVNEVFERDPAALAGIKPGDVITKVDGALVDTPNKLSRLVAALDPG
                     STTRVEVVRDGKRLNLQVALSERKDTVVTASLPQSRTDLKLGIDVQDLTAGLAEKFRL
                     NESRGVLISKVDAGSLAQAEGLREGDLIKEVNRVDTGTVGEFTVAVAKVKRGDTILLR
                     VLREGRAFYVVLKPADH"
     gene            complement(5537..7645)
                     /gene="fusA"
                     /locus_tag="PLS35_09150"
     CDS             complement(5537..7645)
                     /gene="fusA"
                     /locus_tag="PLS35_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNI18870.1"
                     /translation="MMNVPLTESIRNVAVVSNAGSGKTSLVEALACCAGSLSAPGSIQ
                     AGTTVSDFEPEEIHHRTSLHTSVIRCLYNDHVLNLLDTPGSPSCMAELRAALRVADGV
                     VLVVNAATGVRSDLERLWSFIREAGLPCMVFLNDLDREGTTFATTLEEVTKALDMPAV
                     PLILANGEGGQLGGIVDLLQNCVVTGQADRFQAGPSPIPPEWRDRIDQARRGLIEMVA
                     EGDEALLERYLNDGTLTDAALLEGLRVGVQRGALVPVFGGSALRNVGVQTLLQGLLQL
                     MPSPAARAQIAPVQGVGLSDGEPPMTREPVATAPLSGLVFKTIIDPFVGRLSYVRIYS
                     GTLQADSNVYNATRQVRERGGHVFSIFGKKYTAVSRAVAGDIVAIGKLKDSLTGDTLC
                     DEHAPIRLPGIPLGRPVMSFAIEPKSKADIEKVSVGLHKLIEEDPSLEFVRHMETKEM
                     VLSGMGQLHIDVALEKLHRKYGADVTLHAPKIPYRETIRSVAQAQGKYKKQTGGHGQY
                     GDCWVEVGPLPRGQGFEFQNKIVGGAIPRNFVPAVEKGVVEALHEGPLAGYPAVDVRV
                     SVYDGSYHVVDSSEMSFKIAGSMAVKKAMEAAHPVLLEPLMTVEVDVPAECVGAVLGD
                     LNGRRGRIVSVEASGHIETVKALVPQAEMVSYAASLNSLTGGQGSYVMEFASYEEVPR
                     ELASRIVDEHKAERNGVAAH"
     gene            complement(7756..8472)
                     /gene="radC"
                     /locus_tag="PLS35_09155"
     CDS             complement(7756..8472)
                     /gene="radC"
                     /locus_tag="PLS35_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNI18871.1"
                     /translation="MTPKKTGRGIGDWPKTERPRERLLSEGAESLSDAQLLAILLRVG
                     RQDASAVNVGMEVLGRVGGIVGLLHCSTEELCAIPGVGPAKAAQLKAAVEVGKRAVSA
                     PLTTGTRINSSADLFKHYHARLRDLRHEIFAVVLLDAKNQVIRDVTISEGSLTLSIVH
                     PREVFIPAMRASAAGVIFLHNHPSGDPTPSQEDRVLTARLVSAGALLGIQVLDHLIVG
                     DGRYVSFADQGWLGGEASTA"
     gene            8657..8830
                     /locus_tag="PLS35_09160"
     CDS             8657..8830
                     /locus_tag="PLS35_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18872.1"
                     /translation="MECPKCKGMMMLERFSDFFLIFYAWKCINCGAIIDRTISANRRK
                     SLAARDAQPVATR"
     gene            9138..9710
                     /gene="rsmD"
                     /locus_tag="PLS35_09165"
     CDS             9138..9710
                     /gene="rsmD"
                     /locus_tag="PLS35_09165"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNI18873.1"
                     /translation="MRVIAGLHRGRRLLGPRGQAIRPTSDRVKEALFSILGERIVGAR
                     VLDLYAGTGSIGIEALSRGAAHVTFVEADREALRLVQSNLRQCGLEQSANVCACQVSQ
                     FFRRGTQWSGPYDVVFCDPPYQLTPELITLAAEWRAGWLAENAIVILEHGRKADVPSV
                     LGPLSQVKRYDYGDTALTRFHLTPKEVHPA"
     gene            9707..10195
                     /gene="coaD"
                     /locus_tag="PLS35_09170"
     CDS             9707..10195
                     /gene="coaD"
                     /locus_tag="PLS35_09170"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNI18874.1"
                     /translation="MKTAVYPGTFDPITHGHSDIIRRGFRMFSRVIVAIAPNPGKHPL
                     FTTKERLEMVRLVTKDLPNLEVTTFEGLLVDFVRTSGAHAILRGLRAISDFEHEFQMA
                     LVNRKLAETVETVFLMPSEEYSYLSSTIIKDVASHGGSLKDFVHPEVARRLQERIRSF
                     KG"
     gene            10192..11385
                     /locus_tag="PLS35_09175"
     CDS             10192..11385
                     /locus_tag="PLS35_09175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNI18875.1"
                     /translation="MKLAARVGRIVPSPTLSITATAKAMAAQGIDVIDFASGEPDFDT
                     PEPVKAAAEAAIRAGFTKYTPSSGIDELRAAIVDKLKDEQGLQYDKSQILVSCGAKHS
                     LYNVAEALLEAGDELIIPVPFWVSYQDQTLLNDATPVLLQTQERDGYTISQEALEAVI
                     TPRTKAIIVNSPCNPTGATYDRNTLERIAAVALRHDLVIISDEIYEKVLYDGVQHVSI
                     ASLSPEVAARTVVINGVSKAYAMTGWRIGYAAGPKPLLTAMANIQSQSTSNPCSISQK
                     AAVAALRLGNPFTTVMVAEFDRRRRLMVERLNKMPGMTCRMPTGAFYAFPNVSGLLTK
                     RWNNQPIGSAANLATFLLNEAQVALVPGEPFGSDVHIRLSYATSMEAIERGLTRIEAA
                     IRRLS"
     gene            complement(11682..11873)
                     /locus_tag="PLS35_09180"
     CDS             complement(11682..11873)
                     /locus_tag="PLS35_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18876.1"
                     /translation="MLELLLEAEVVDVVAAGAGAAGVDVEAGLLLVVSDEAGVFALSV
                     PVAAGLSEVSLPPGFILSE"
     gene            complement(12036..12479)
                     /locus_tag="PLS35_09185"
     CDS             complement(12036..12479)
                     /locus_tag="PLS35_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18877.1"
                     /translation="MLIRSRRAVIGLSMLGFSSMLLLAGCDFWPPALQAQIEQLHSEA
                     QQAAADKALLQNQLNAANKAKEELQIRVEDLTRVNKEKSAMIASLEHTIAAARERAVK
                     AAKAPAAKPAAKTTVKKPVKAPSKATSKSSAKSSTVKKPKSAQLR"
     gene            complement(12528..13175)
                     /locus_tag="PLS35_09190"
     CDS             complement(12528..13175)
                     /locus_tag="PLS35_09190"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNI18878.1"
                     /translation="MALVLPFIDKTFDSILPEVERVLAAQGLIALPTETYYGLAVRPS
                     DEVAVRRLIDVKGRPADKPILVLIGGRDQLPTLVSSIPPAAAALMDLFWPGPLTIVFP
                     AATGLSPLLTAGTGTIGVRWSPLATLQRLLKRTGPLTGTSANRSSEPPLDQAASVQHT
                     LGPVLDLILDGGRTPGGSASTIVDACGEPRVLRAGVVSTERIRAALAQRGHTLSS"
     gene            complement(13197..14474)
                     /gene="purD"
                     /locus_tag="PLS35_09195"
     CDS             complement(13197..14474)
                     /gene="purD"
                     /locus_tag="PLS35_09195"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNI18879.1"
                     /translation="MKILVVGSGGREHALVWKIAQSPRKPQIFCAPGNAGIEGLATCV
                     PVKADDIAGLKAFALKEGIDLTVVGPEAPLALGIADEFRKARLKIFGPTKAAARLESS
                     KSFSKDIMAASRIPTAVSKSFEQMDQALAYVDAQPVPIVVKADGLAQGKGVVVAMTRE
                     EAKQAVRDAMEKSVFGQAGQRVLIEEFLDGEELTIMAFTDGNTVVPMIPAQDHKRVGD
                     GDAGPNTGGMGAYAPAPIATAALRDQVTRQVLQPTVDAMARLGCPFQGVLYAGLMIVK
                     GTPYVLEFNARMGDPETEVVLPLLKTDLVDVMEAVVEHRLDELQVEWRQDTAVCVVMT
                     SPGYPGSYETGFPIEGLPAQSGASPIAVFHAGTKREAGQVVTAGGRVLAVAAWAPSLL
                     QARAQVYQAVPGIRFQGRHYRTDIAHRALPRQS"
     gene            complement(14693..16246)
                     /gene="purH"
                     /locus_tag="PLS35_09200"
     CDS             complement(14693..16246)
                     /gene="purH"
                     /locus_tag="PLS35_09200"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNI18880.1"
                     /translation="MASIARALISVSDKTGVVEMAKGLAALGAEILSTGGTAKALRDA
                     GVAVTDVAAYTGSPEILDGRVKTLHPKIHGGLLGRRGVPDHVKQMQQHGIGNIDVVVV
                     NLYPFESTIAKPNCPFEEAIENIDIGGPSMLRSAAKNHEDVLVVVDPSDYTRVLEAVK
                     AGSVTPALRRELAMKVFQHTARYDSLIAGYLEKQVQGSEVKFPAILSLQFERVETLRY
                     GENPHQQGAFYREMQAKEPAVSRGKILHGKAMSYNNFLDSNSALELVKEFDQTAVAII
                     KHNNPCGCALGATPVEAYVKARATDPVSAFGGVIAFNRPVDLAAAKEITSTFVEVVIA
                     PGFAEDALAELKRKKDIRLLDVGPLSKATAEGYDLKKLVGGLIVQDRDLGVIKDIKAL
                     AVPTSRKPTEQEYAACAFAWVVCKHVKSNAIIYGRPGEIVGIGAGQMSRVDSVKLAAM
                     KAQSSVKGCVMASDAFFPFRDGIDAAAEAGITCVIQPGGSIRDPEVTKAVDEHGMAMI
                     LTGMRHFRH"
     gene            16591..17502
                     /locus_tag="PLS35_09205"
     CDS             16591..17502
                     /locus_tag="PLS35_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI18881.1"
                     /translation="MSRGIIFGVGLFALTLLALICIPRHLPVTSSATGHPSFSAHIEN
                     GHLTLSGAMGSEEAKLAAVARAQELAKNLKLRVTDNLDVLEDGGAAAWEAAVPALLTQ
                     VAALHQHQASLTVSRQTATIKGAASNADAKTKLVHEMSSLLGTAMHVQDQVIVTSAGS
                     ASPSSQPTPTLTATPPTAHASRAQVQASLDEIVRGEHIAFESNSAVLTPKGRAVIDKL
                     IPALKRSPESAIEIGGHTDSYGDPDYNLQLSRTRAESVRQYLADHGVTNRLTAVGYGA
                     SRPLSQDRTRAASKKNRRIEFRVKEER"
BASE COUNT         3592 a         5444 c         4988 g         3539 t
ORIGIN      
        1 ccgcatcctt tgtttagcgg atttgtgggg gcggcactac gccgcaaatg cggccactaa
       61 atgggatgct gaaactgcct ttctgcggcg ttctcgactc ggcacagtcc tcgacgtagc
      121 tccgctacgc ctccggctct gcctcgtctg cggccttgct gaaagatcag tttgagcatc
      181 ccgcgaattc agaagagaag actatggcgt acgaagttga tctcggacaa tttaagatcg
      241 gagtggggag tcggccgttt ttgatcgcgg ggccttgcgt gattgaaagc gagcaactcg
      301 tcatggatac ggcgggacgt atcgccgaaa tcaccaaatc actcggcatg ccctacgtgt
      361 tcaaatcctc ctttgataag gcgaatcgca cctccatctc ctcctttcgg ggacccggct
      421 tagaaaaggg tcttgccgtt ctggccaagg tcaagcagca gatcggcgtg ccgattctta
      481 ccgacgtcca ttcggaagag caggcgacag aggccggcca tgtcgtagac atgctgcaga
      541 ttcccgcttt tctctgccgc cagaccgacc tgttgattgc cgccgcgaag accggcaagg
      601 tcgtgaacat caagaagggg cagttcctgt cgccgccgga aatgggcaac gccgtcaaga
      661 aggtcgaaga cgccggcaac cgccgtatcg tcctgacgga gcggggctcc tcgttcgggt
      721 acaacaatct ggtcgtcgac atgcgctcat ttcccatcat gcgccgcttt ggctatccgg
      781 tggtctttga tgccactcat agtgtacagc tgcctggcgg gggcggcacc aaatccagcg
      841 gccagcgtga attcgttgaa cccttggcct gcgccgcggc aggagccggc tgcgacgggt
      901 tcttcatgga ggtgcacccg gatcccgatt ccgctctatc cgatggaccg aacatggttc
      961 cgcttcatgc gcttcattca ctccttgaac gggtattacg tatatgcgac gcagccgctc
     1021 cacaaccagt ccggtaacgc gcaagaccaa gacatccgcc gggcgttctg ccgccgtcga
     1081 tgcgagcgtg cgcgagggca ttcgcgtgct cgacatcgaa gcgcgcgcag tgctcgatct
     1141 caaggcccgt ctggatgagc gattcgccag agccgtgacg ctgctgtacg agtgtcaggg
     1201 gaaagtcgtc atttccggca tgggaaaatc agggctcatc ggacaaaaaa ttgcagccac
     1261 gttggccagt accgggacac cgtccttctt tctgcacccg gccgaaggag tccacggcga
     1321 tctgggcatg ctcgcacggc gtgatgtgct catcgccatc tccaacagcg gggagacgca
     1381 ggaagtcctt caactgctac cgttcgtcaa acgaatgaat gtgcctgtcg tcggcatgac
     1441 ggggaagatg acctccacgt tatcgaaaaa cagcgatgtc acgttggatg tgtccgttga
     1501 tgaggaagcc tgccccctgg gcctggctcc gaccgccagc acgaccgcca cgctcgcgat
     1561 gggtgatgcc ctggctgtgg ccttactgca aaaacgcggg ttcaaacatg acgacttcgc
     1621 acaatttcat cccggcggca gcctggggcg tcgtttgctc gtcaaggtgc gtgatctcat
     1681 gcaacatggg gatcatttgc cacgcgtgcg cgccgacgtg tccggggcgg acacgatcct
     1741 ggagatgacc tcgaagaaac tcggactgac gacggtggtc gatgccaagg gatcgctata
     1801 cggcattgtg accgatggag acttgcggcg cttcattcaa gccggcggcg atttcagccg
     1861 ggtcacggcc ggtgacctgg ccagccgcca tccgaagacg atcggtccgg atgaattggc
     1921 tacgacggcc gtcgcacaga tggagcgttt ctccatcacg gcactcgtgg tcatcgagcc
     1981 accgaatcgc ctggccgggg tcattcactt gcatgacctg ctgaaacacg gcattgtata
     2041 aaggaacctt cgcgaacatc ggagacttcg ggcgacacag accatgggag agaactggaa
     2101 agaagcagga ctggtcctga tgcggtcggc agggcaggaa agtaacatca acctgcccaa
     2161 cgtgctgacg ctcgtccgca tcctgttgat tccagtgttc gtcatgctgc tgatcgatcc
     2221 gacgccagac cgcgccttga gcgccgccat tgtgttcgtc gtggcggccg tgacggatct
     2281 gctggatggc tacgtggcca gaaaaaccgg ccagatcacc aagctggggc gccttctgga
     2341 tcccattgcc gacaagctgc tggtgttgtc tgccttgatt ctgctggtgc aagtcgatcg
     2401 cgtcagcgca ctggtagcca tcctgattat cgcgcgcgag gtggccgtga ccggcttgcg
     2461 cgcgatcgcg gcctccgaag gattgatcat gtctgccgag gtcacaggca aatacaaaat
     2521 ggcgttgcag gtcatcgcca tcgtccttct ggtgctggaa ggcaccgtcg tggaaaccat
     2581 cggcaatctg catctcgccg gcatcgtcac cctctacctc tcgttgatcc tgggatatgt
     2641 gtcgggcgca cagtatgtct ggagcttctg gcggcaggtg ggagcaaaag gtctgtaggt
     2701 gaacaacccg gcggcacgca ctcggtgatg gacagtccct ggctcatcgg tgttctcatc
     2761 atcttcgcct atctccttgg atcggttccg tttggcgtcg tcgtgtcgcg cctgctgggc
     2821 gccgtcgatc cgcgaaccgc cggcagcaag aatgtcggct tcaccaatgt tctgcgcgtg
     2881 agcggcaaaa aagccggtgt cctgacgctg gcgggggaca tcggcaaagg ctggctggca
     2941 gggtgggtgg ccacgatggt gctgcagcag gaaacggcca ttttaggggt cgccctggcg
     3001 tcaattctcg gccatttgca ctcgatattc cttggattga aaggcggcaa aggcgtcgcc
     3061 acagcgttag gggcggcact gggcgtcgcc ccttgggtcg ggctcacact catgacgctc
     3121 tggatcggcg ccgtgttctt gtggaagtat tcttccggag gcgcgctgtt tgcctttggc
     3181 atgttcccga ttgtggcgct gatcttccat cgctcctgga cgtttatcgg atttgcctgt
     3241 gtcgtcagtg tgctgatctg gatgaggcac aaggacaatt tgatccgatt gtggagcggg
     3301 acggaatctc aaatcggaca atcgagttga cataatatat cttatcagac attaccgtta
     3361 tgctgtcttt tgagctattg gctcgcatcc gcaaagagct gttcctcacc ggcagcgccc
     3421 tctacgagac gattgtcgcc atcgcggaac gcgtcaaccg caaagtccat gtcttgcgcc
     3481 tccatggcca ggccacgacg atcctgaacc agacgcacgc cattcatcgc cagatcggac
     3541 aacgcgttgc cgctctcgca gccaaaccgg ctgcgggaat ccatccggta gcgccttccg
     3601 aattggcctc gctgcaggac gcaatccgga ccgcctccga ccgcatcaag ggccaacaag
     3661 ccgcgttgca acttgtggaa cgccagatcc gtgagctcaa ggtcgagctg gctcacgaag
     3721 acctgttgac gatgcaacgc gaattgggtc tccgcgacgc cgccattgaa cgcgtggtgg
     3781 tcgcccgagg cgcgccggtc atcggccatc cgatcggcga attcgacctc ccgcccacga
     3841 cccgcatcgc cgccgtcttt cgcgggccgt ttcttctgcc tctctcccac accatgacgc
     3901 ttcgtcccga tgacatcgtc atccttgtcg gccttcgcca cgacttggcc cactggctcc
     3961 cgcacttcca gcacccggcc gcatccaaat cagcctgacc cgtccttcct ttcgccgtca
     4021 cgcgaaaatc tggtattctg aggcgtcgcc agagtgcttc ggtatcgccc tcggagcttg
     4081 cctcaacatg aaaacaccgc tgtcatgttc taccctgctg tgtcttggac ttattctgac
     4141 tggatcgttc ctgggaagcc ctttgtccgc cgtggctgca gccgacgtcg cctccacgcc
     4201 acgcaccgtg tcgccaggat tacgcctgtt ggaagagctg cagaccgtca tcactgacct
     4261 cgccgaacag gccaagccat cggtcgtcag catctttccg atccaggcca ccggccgatc
     4321 tcgagacgga ggcgagcggg ttcccagctc gaccggatcg gggtcaggcg tcatcgttga
     4381 tgccgccggc cacatcatta ccaataatca tgtggtgggc gacgcggcgg aagtcgaggt
     4441 tcgcctctca gacaaaacga agttgtttgc gcaggtgatc ggtaaggatc cggacaccga
     4501 cctggccgta ctcaaagtca ccgccgacca tcccctgccc gcggcccatt tcggtgactc
     4561 gtccggcgtg aaggttggcc agtgggtgct ggcggtcggg aatccgttcg gcctggatcg
     4621 caccgtaacg ctgggggtgg tcagcgggat cggacgtgag aacatcaatc tctcccgcta
     4681 cgaaaatttc attcagactg acgcgtcgat caatccaggc aactccggcg gcccgctgtt
     4741 tgatctccgt ggggatatca tcggcatcaa taccgccatt atcaattttg cgcagggcat
     4801 cggtttcgcc atcccgtcca acatggccaa gcaggtcatg aaccagctga tcgcgaaagg
     4861 caaagtcgtg cgagcctggt tgggggtcgg gttacaaccg ctgacgccgg aactggccag
     4921 caagttcggg gtggatgaga acgaaggggt tctggtcaat gaagtgttcg agcgggatcc
     4981 ggcggcgttg gccggcatca agcctggaga cgtcatcacg aaggtcgatg gggcgctcgt
     5041 ggatacgccg aacaaactgt ctcgcctcgt tgccgcattg gaccccgggt caacgacgcg
     5101 tgtcgaagtc gtacgcgacg ggaaacgcct caatctccag gtcgccctga gcgaacgcaa
     5161 ggatacggtc gtgaccgcct ccttgccgca gagccggacc gatttaaaat tgggcatcga
     5221 tgtgcaggac ttgacggccg gcctggcgga aaagtttcga ttgaatgaaa gtcgcggcgt
     5281 gctgatttca aaggtggacg caggaagcct cgcccaggca gaaggattgc gggaaggcga
     5341 cctgatcaag gaggtcaacc gcgtggacac cggcacggtc ggggaattta ccgtggcggt
     5401 tgccaaggtc aaacgcggcg acacgattct tctgcgcgtc ttacgggaag gacgtgcgtt
     5461 ctatgtggtg ctgaaaccag ccgatcacta accgatttca acaaggccgc atcgcgtcag
     5521 gtggggacgt ggcccgtcaa tgggccgcaa ccccattgcg ctccgctttg tgttcgtcaa
     5581 caatccggga cgccagctcc cggggcacct cttcatagct tgcaaattcc atcacataac
     5641 tgccctgccc gccggtcagc gaattcaacg aggcggcata gctgaccatt tctgcctggg
     5701 gtacgagagc cttcaccgtt tctatatggc cactggcttc caccgaaaca atccgtccac
     5761 gccggccgtt gagatcaccc agcaccgcgc cgacgcactc cgccggaacg tcgacctcta
     5821 cagtcatgag cggttcaagc agaaccggat gggccgcttc catggctttt ttcaccgcca
     5881 tcgatccggc gattttgaac gacatttcag aggaatccac cacatggtat gagccgtcgt
     5941 agacggacac ccgcacatcc acggcgggat atcccgccaa tgggccttcg tgcagtgctt
     6001 ccacaacccc cttctcgacg gccggcacaa aattacgcgg aatggcgccg ccgactattt
     6061 tgttctgaaa ctcaaatccc tggcctcgcg ggagcggacc gacttccacc caacagtccc
     6121 cgtattgtcc atgtccgccg gtttgtttct tatattttcc ctgtgcctgc gccacgctgc
     6181 gaatcgtttc gcgataggga attttcggcg catggagcgt cacgtcggcg ccatacttgc
     6241 gatggagttt ttccagcgcg acatcgatat gcaattgtcc cattccgctc agcaccattt
     6301 ctttcgtttc catgtgccgg acgaactcta agctggggtc ttcttcgatc aacttgtgca
     6361 acccgacact caccttttcg atatcagctt tggatttggg ctcgatggca aacgacatca
     6421 ccgggcgacc aagtggaatc ccgggcagcc ggatcggagc gtgctcatca cacagcgtgt
     6481 cacccgttaa gctatctttc agcttcccga tggccacaat atcgcccgcc accgcccgtg
     6541 aaacagccgt gtactttttc ccgaagatcg aaaacacatg ccccccgcgc tcccgaacct
     6601 gtctcgtcgc gttgtacaca ttgctatccg cctgtaacgt gcctgaatag atccgcacgt
     6661 acgacaggcg cccgacaaac ggatcgatga tggtcttgaa taccagtccc gagagagggg
     6721 cggtcgcgac cggctccctc gtcatgggcg gctcaccatc agagagtccg actccctgca
     6781 cgggtgcaat ctgagctcgg gcagccggag acggcatgag ttgaagcagg ccctgtaaca
     6841 atgtctgcac ccccacgttg cgcaacgccg atcccccgaa cacggggacc agcgcgccgc
     6901 gctggacgcc gacccgcaat ccctccaaca aggcagcgtc cgtcaatgtc ccatcgttca
     6961 ggtaccgttc aagcaacgcc tcgtcgcctt cagcgaccat ctcgattaag ccgcgccggg
     7021 cctggtcaat ccgatcgcgc cattcgggtg gaatcggaga gggccccgct tgaaatcgat
     7081 cggcctgccc cgtcacgaca cagttctgca acaaatccac gatgcccccc aattgcccgc
     7141 cctccccatt cgcaaggatc aacgggacag caggcatgtc cagcgctttc gtcacctctt
     7201 cgagcgtcgt ggcgaatgtc gtcccttccc gatccaaatc attcaggaac accatgcagg
     7261 gaaggccggc ctcacggatg aacgaccaga ggcgttccag gtcactgcga actcccgtcg
     7321 ccgcgttcac aacgagcacc acgccgtccg caacacgcaa tgccgctcgc agttccgcca
     7381 tgcaacttgg ggagcccggg gtgtcgagaa gattgagtac gtggtcgttg tagaggcaac
     7441 gaatgacgct ggtgtggagg gatgtccggt gatggatttc ttccggctca aaatcggaga
     7501 cggtcgtgcc ggcctgaata gacccgggag cggaaaggct gcctgcgcaa caggcgagcg
     7561 cttcgactaa tgaggttttg cctgatcccg cattcgatac cacagccaca ttcctgatgg
     7621 attcggtgag gggaacattc atcatgacct cctccggtag tgggcggccg ctggggcctg
     7681 gttagagaga gacctgtggg cgacaccatc ctttcttcat ccgtacgaat tcgtcccgtg
     7741 gtgacaggca gtccgtcatg ctgttgaggc ctctccgcca agccatccct ggtcggcgaa
     7801 actgacatac cggccgtctc ccacgataag gtgatccagg acctgaatgc ccagcagcgc
     7861 ccccgctgaa acgagcctgg ccgtcagcac acggtcttcc tggcttggag tagggtctcc
     7921 gctggggtga ttatggagaa aaatgactcc ggcggctgac gcacgcatgg cggggatgaa
     7981 gacttcccgc gggtgcacga tgctcagcgt gagactgcct tctgaaatcg tcacgtcgcg
     8041 aatgacctga tttttcgcat ccagcaatac gacagcgaaa atctcgtgac gcaggtcccg
     8101 taagcgtgcg tggtaatgct taaagagatc cgcgctggaa ttgattcgcg tgccggtcgt
     8161 caatggcgcc gaaacagctc gtttgcctac ctccaccgct gccttcaatt gcgcggcctt
     8221 ggccggccct accccgggaa tcgcgcagag ttcttcggtg ctgcagtgca gcagcccgac
     8281 aatgccaccg acgcgcccca gcacctccat gccgacgttg acggccgacg cgtcctgccg
     8341 tcccacgcgc agcagaatcg ccaataattg cgcatcagac aggctttcag ccccctcact
     8401 gagcaatcgt tcgcgcggcc gttcagtttt tggccaatct ccgattccac ggccggtctt
     8461 ttttggtgtc atggcggctt cctacgacaa aaaaatgtca gcgtgcacga ttttgtgccg
     8521 gagacttgac gcctggcgca tgtcatatat ggcactttcg atgcgtaaca tattgaaatt
     8581 aaaagatttt acattttggt gcagctcttg cttgagagca gacacagctg taaacgtcaa
     8641 cacccaggag gaaaccatgg aatgtccgaa gtgcaaaggt atgatgatgt tggaacggtt
     8701 ctcagacttc ttcctcattt tctacgcctg gaaatgcatc aactgcggag ccatcatcga
     8761 ccgaaccatt tcggccaatc gtagaaagag cctcgccgct cgcgacgctc aaccggtggc
     8821 gacccgctaa acgcggagcc ctcgctctgt tcgctctgtg tgatggggtg gtgtgacggc
     8881 ccaggattgg ccacctcctc agcccgcgtg aaccgagtat tggtgtcgcc cgattatagc
     8941 gagcctcctt ttgcgcgtca aagcgcgtac actcctcctt gacgtctaca gcgctccccg
     9001 ccccgcggcg gggagaagac ctttccggct cacggccgct cctcgccgcc tccagtcacc
     9061 ttgacccctt caaaaacgct gtgttacatt ctctcacttc ttagccagag acgaatggct
     9121 ctcacctgaa tacgatcatg cgcgtcatcg ctgggctgca tcggggccga cggttgctgg
     9181 gtcccagggg acaggccatc cgcccgacat ccgaccgagt caaagaagcc ctcttttcta
     9241 tccttggcga acgtatcgta ggggcgcgcg tattggatct ctatgcgggg accggctcaa
     9301 ttggcattga agcgctgagc cgcggggcgg cgcatgtgac ctttgtggaa gctgatcgcg
     9361 aggcgctacg cctggtccaa tcgaatctgc ggcaatgcgg gctggaacaa tccgccaatg
     9421 tgtgcgcctg tcaggtgagt cagttttttc gtcgcggcac gcaatggtcg ggtccttatg
     9481 acgtggtctt ctgcgatcca ccctatcaac tcacacctga attgattacg ctggcggcag
     9541 aatggcgtgc cggttggctg gcggagaacg ccatcgtcat cctggaacat ggacgaaaag
     9601 cggacgtgcc ctccgtcctg ggaccacttt cgcaggtgaa gcgctacgac tatggcgaca
     9661 ctgcactgac ccgctttcac cttacgccga aagaagttca cccggcatga agaccgccgt
     9721 ctatcccggc acattcgatc cgatcacgca tgggcacagt gatatcatcc ggcgcggatt
     9781 ccggatgttc tcgcgggtca ttgtggccat cgccccaaac ccgggtaagc acccgctgtt
     9841 caccacgaag gaacgattgg agatggtgcg cctcgtcacc aaagatcttc ccaacctgga
     9901 ggtcaccacc ttcgagggac tgttggtgga ttttgtgcgc accagcggcg cccatgctat
     9961 tctgcgggga ctgcgcgcga tttctgattt cgagcacgag tttcaaatgg cactggtcaa
    10021 tcgaaaactg gcggaaaccg tggagacggt cttcctcatg cccagtgaag aatattccta
    10081 tctctcttcc accatcatca aggatgtcgc cagccacggc gggtcactga aagactttgt
    10141 gcacccggaa gtggcacgga ggctccaaga acgaattcgg agtttcaaag gatgaaactg
    10201 gcagcccgtg tcggacgaat tgtgccctct ccaaccctga gcattaccgc caccgcaaaa
    10261 gccatggcgg ctcaaggtat cgacgtcatc gattttgcct ccggcgaacc ggactttgat
    10321 acgccggaac cggtgaaagc cgcggccgag gccgccattc gagcaggctt tacgaagtat
    10381 acgccgtcgt cagggatcga cgaactgcgt gccgcgattg tcgataagtt aaaagacgaa
    10441 cagggacttc agtatgacaa gtcccagatt ctggtgtcct gcggcgccaa acattccctc
    10501 tacaacgtgg cggaagcgtt gttggaggcg ggcgatgaac tgattatccc ggtccccttc
    10561 tgggtgtcct accaggatca aaccctgctg aacgatgcca ccccggtcct gcttcagaca
    10621 caggagcggg atgggtacac gatttcgcaa gaagccctgg aagccgtcat caccccgcgc
    10681 accaaagcga ttatcgtcaa cagcccgtgt aatcctaccg gcgcgacgta cgaccggaac
    10741 actctggaac gcatcgccgc cgtcgctttg cgtcatgacc tcgtcatcat ttcagacgag
    10801 atttacgaga aggtgttgta cgacggcgtg cagcacgtca gcatcgccag cctgagcccg
    10861 gaagtcgcag cacgcacagt ggtcatcaac ggtgtttcga aagcctatgc gatgaccggt
    10921 tggcgcatcg gctatgccgc gggtccgaag ccgttgctga cagcgatggc gaacatccaa
    10981 agtcaaagca cctcgaaccc ctgctccatt tcgcaaaaag ccgccgtggc ggcgctccgt
    11041 ttgggaaacc cctttaccac tgtgatggtg gcggaattcg atcgtcggcg acgcctgatg
    11101 gtcgagcgcc tgaacaaaat gcctggtatg acctgtcgca tgccgaccgg agcattttat
    11161 gcgttcccca atgtgagcgg cctgctgacc aagcgatgga acaatcaacc catcggctcg
    11221 gccgcgaacc tggcgacctt tttgctcaac gaagcgcagg tggcgttggt gcctggtgaa
    11281 ccgtttggaa gcgacgtcca tatccggctc tcctatgcga cctcgatgga agcgatcgaa
    11341 cgcgggttga cccgcatcga agccgccatt cgccggctgt cgtgatcaac aggatctcga
    11401 cacctcggcc ccttgactgc tctcgacacg atgttatcag gggagaggtc ttgcagctga
    11461 cggatcgagc caatcaagac cgagtgacgc cctaggatca gaggaggaag acccgtgcca
    11521 atctatgagt atcaatgttc cagctgtgcg catcgcttcg aggtgaagca gagcatcaag
    11581 gatgacccca tcaaggaatg cgtacgctgc ggcaaagaag tgacgaagct gatttcgccc
    11641 cctgccatta tgtttaaagg cagcggttgg tatatcaccg actactcgga caaaatgaaa
    11701 ccggggggca gcgatacctc cgacaagccg gcagctaccg gcacagacaa ggcgaaaacc
    11761 ccggcctcgt cggataccac aagcagcagc cccgcctcca cgtcgacgcc tgcagcgcct
    11821 gcgccggccg cgacgacatc cactacttcg gcttcgagca gcagctcgag cacatccagc
    11881 cccagtgcag cggggtctca gtcgtcagcc ccatcctcca gtaaatcgtg agaaggctct
    11941 tgccttcacg cgatgagagt ggtcgatcgg ctcgatcttt ctgacgtggg accaaccgaa
    12001 cctgctggcc gggctgtacg ctgacttgag caggcttatc gaagctgggc agatttcggt
    12061 tttttgacgg tcgaggattt ggcagaggat ttcgacgtcg ctttcgatgg cgctttgacc
    12121 ggcttcttca ccgtcgtttt tgcggcaggc ttggccgcag gagccttggc ggccttcaca
    12181 gcccgctccc tcgctgcggc aatcgtgtgc tccaaactcg caatcatcgc cgacttttct
    12241 ttattcaccc gcgtgagatc ttcgactcga atttgcagct cttctttggc cttattcgcc
    12301 gcgttgagct gattctgcaa gagtgcctta tccgccgcag cctgttgcgc ttccgaatgt
    12361 aattgttcga tttgggcctg gagcgccggc ggccaaaagt cacatcctgc cagcagcagc
    12421 atactgctga acccgagcat cgacagtcct atcaccgcgc gacgtgaacg aatgagcata
    12481 agtggtcctc ccgtgtgggt gaattatacc cacaacgcac catcaaatca tgatgaaagt
    12541 gtgtgtcccc gttgagcgag ggccgcacgg atccgctccg tcgacaccac ccccgctcgc
    12601 agcacgcgcg gttcgccaca ggcatccacg atggtggacg ctgatccacc cggcgtgcgt
    12661 ccgccgtcca gaatcagatc gagaaccggc ccgagagtgt gctgcacaga cgcggcctga
    12721 tcgagaggcg gttcagatga ccgattggcg ctggtccctg tcagcggacc agtgcgcttg
    12781 agcagacgct gcagcgtggc cagcggtgac catcgaacgc cgatcgtgcc ggttcccgcc
    12841 gtcaacaacg gtgacaagcc ggtcgcagca ggaaatacga tcgtcaaggg acccggccaa
    12901 aacagatcca tcagcgcagc ggcggccgga ggaattgagg acaccaacgt cggtagctga
    12961 tcgcggccac cgatgagtac gagaatcggc ttgtcggccg gccggccttt gacgtcgatc
    13021 aggcggcgca ccgccacctc gtcgctgggg cgaaccgcca atccataata ggtttcagtc
    13081 ggcagtgcga tcagcccctg cgcggccagg acacgttcga cctcagggag gatgctgtcg
    13141 aaggtcttgt cgatgaaggg aaggaccaga gccatgtcag gcctgtcgag cgcggttcaa
    13201 gactggcgtg gaagggcgcg gtgggcgatg tctgtgcgat agtggcgtcc ctggaagcga
    13261 atacccggca cagcctgata gacctgtgcc cgcgcttgca gtaaagacgg agcccaggcc
    13321 gcgactgcca acacgcgccc cccggccgtt accacctgcc ctgcttcccg tttggtcccg
    13381 gcgtgaaata ccgcaatggg cgacgcccca gactgcgccg gcaaaccttc aatagggaac
    13441 ccggtctcat aggaaccggg ataccccggt gaggtcatca ccacacagac ggcggtgtcc
    13501 tgccgccatt caacctgcag ctcgtcgaga cgatgttcaa ccaccgcctc catcacgtcg
    13561 accagatcgg tcttcagcaa gggcaacacg acttccgttt ccggatctcc catacgtgca
    13621 ttgaattcca gcacgtaggg agtccccttc acgatcatga ggcccgcgta cagcacgcct
    13681 tggaaaggac agcccagacg ggccatcgca tccacggtcg gttgcagtac ctgccgcgtc
    13741 acctggtcgc gcagcgcagc cgtcgcaatg ggagccggag cgtaggcgcc catgccaccg
    13801 gtattgggtc cggcatcacc gtcgcccacc cgcttgtgat cctgcgcggg gatcatcggc
    13861 acgacagtgt ttccatccgt aaatgccatg atggtcagct cctcgccatc gagaaactct
    13921 tcaatcaaca cccgttggcc ggcctggccg aagacggatt tctccatggc gtcgcgcacc
    13981 gcctgtttgg cttcctcgcg cgtcatcgcc accactacgc ccttgccctg cgcgaggccg
    14041 tcggccttca ccacaatcgg cacgggttga gcatccacat acgccaacgc ctgatccatc
    14101 tgctcgaagc ttttcgagac cgccgtggga atccggctgg ccgccatgat gtccttcgag
    14161 aagcttttgc tggattccag ccgtgccgca gccttggtgg gaccgaagat cttcaaacgt
    14221 gctttcctga actcatcggc aatgcccagc gccagcggcg cttcaggacc aacgacggtc
    14281 agatcaatgc cctccttgag agcaaaggcc ttcagcccgg caatatcatc tgccttgacc
    14341 ggcacacagg tcgccaggcc ttcgatgccg gcattgcccg gcgcgcaaaa gatctgcggc
    14401 ttccgcggac tctgggcaat tttccagacc agggcatgtt cccgaccacc actgccgacg
    14461 accagaattt tcacagctca acctttagtt gaaatgacga tagacggatc tcacacagaa
    14521 ttgaccgcag acttcaagaa ccgcggacag ccatcctcgc tctgccgagg atgctcaaac
    14581 aggttttcca acaaggccgc aggcgagaaa aaaccggagg cgtagcctct gggctacgtt
    14641 gaggattttt tcgagccgag aacgaagttg gaaacctgtt tcagcatccg atctagtggc
    14701 ggaagtggcg catgcctgtg agtatcatcg ccatcccgtg ttcatctacg gccttggtca
    14761 cctcggggtc acgaatagac ccgccgggct gaatcacaca ggtgataccg gcttctgccg
    14821 cggcatcgat gccgtcccgg aacgggaaga acgcgtcgga cgccatgaca cagcccttca
    14881 cggacgactg cgctttcatc gccgccaact tcaccgaatc cacccggctc atctgtccgg
    14941 cgccgatgcc gacgatttca ccaggccgac cgtaaatgat ggcattcgac ttcacatgtt
    15001 tacagaccac ccaggcaaat gcacaggccg catattcctg ctcggtgggc ttgcgtgagg
    15061 tcggcacagc gagtgctttg atatccttga tcacgccgag atcccgatcc tggacgatca
    15121 atccaccgac cagcttcttg agatcatatc cttccgcggt cgccttgctg agcgggccga
    15181 catccagcag acgaatgtcc ttcttccgct tgagttccgc cagcgcatcc tcggcaaaac
    15241 ccggcgcgat gacgacttcc acaaacgtcg acgtaatctc cttcgccgct gccagatcga
    15301 ccggacgatt aaacgcaatc accccgccga acgccgacac aggatccgtc gcgcgcgctt
    15361 tgacgtaggc ttccacgggc gtggcgccga gggcgcagcc gcagggattg ttgtgcttga
    15421 tgattgccac ggcggtctgg tcaaactcct tcacgagttc gagcgccgaa ttggagtcga
    15481 gaaaattatt gtacgacatg gctttgccgt gcaggatctt gccgcgtgac acggccggct
    15541 ccttggcctg catctcacga tagaaggccc cctgctggtg cggattttca ccgtagcgca
    15601 gcgtctccac ccgctcgaac tgcaaggaga gaatggcggg gaacttcacc tcgctccctt
    15661 gcacctgctt ttcaagatac ccggcgatca gactgtcata gcgcgccgtg tgctgaaaga
    15721 ccttcatcgc cagttcgcgg cgcaatgccg gcgtgacgga accagccttc acggcctcca
    15781 atacgcgggt gtaatcagac gggtccacca cgacgagcac atcctcatgg ttttttgcgg
    15841 cggagcgcaa catggaggga ccgccgatgt cgatgttctc gatcgcctcc tcaaacggac
    15901 aattgggctt ggcgattgtc gattcgaagg gatacagatt caccacgacc acatcgatgt
    15961 tgccgatgcc gtgctgctgc atctgcttga cgtggtcggg aacgccccga cgccccagca
    16021 aaccgccgtg aattttcgga tgcagcgttt tcacgcggcc gtcgagaatc tccggcgacc
    16081 ccgtataggc agccacatcc gtcaccgcca cacccgcatc acgcaaggct ttggcggttc
    16141 cgcccgtgga caatatttcc gcgccaaggg ctgccaaccc tttggccatc tcaacaactc
    16201 cggtcttgtc agaaacactg atcagtgccc gcgcaatgct ggccatgatg aactccttca
    16261 actatggata tcgtgtgggt gggatcacat tggacgaaaa aacgccgtga caatagcaga
    16321 tgcctcgacg ccactgcaag ccgatcgagt atgccatcac cgcatcagcg ctgtggcaga
    16381 atccgatgag ggaatcagga ggacgtccgc tacccagaat ccaggctagc atgagtccca
    16441 gcgatgtgat acccttcgca attcgtaatc gagcagccgg cgctgcctaa acaggaaatc
    16501 aagtaacact cgaagtcttc aaccactcca catcctgtgg cgttcgacgc cgcagcgagg
    16561 cctcgtcccc gaggcgataa gggctggcgc atgtcgcgcg gaattatttt cggcgtagga
    16621 ctctttgcac tcacattatt ggcgctcatt tgtatccctc gacacctgcc ggtgacgtcg
    16681 tcggccacgg ggcatccctc gttcagtgcc catatcgaga acggtcacct gacactttcc
    16741 ggggcaatgg gaagcgaaga ggccaaactc gccgccgtcg cacgtgcgca ggaactcgcc
    16801 aagaacctca agttgcgtgt caccgacaat ctcgacgtgc tggaggacgg cggagcggca
    16861 gcatgggaag ccgcagtgcc tgccttgctc acgcaagtgg cggccctgca ccaacaccag
    16921 gccagcctca ctgtctcgcg ccaaacagcg accatcaagg gcgcggcctc gaacgcggac
    16981 gccaaaacga aactcgtgca tgagatgagc tcgctgctcg gcaccgcgat gcatgtgcag
    17041 gaccaggtaa tcgtcacctc ggccggctcc gcctccccgt catctcaacc gacgccgaca
    17101 ctgaccgcca cccctccgac ggcgcacgcc tctcgcgcgc aagtgcaggc cagcttggat
    17161 gagatcgtgc gcggagaaca tatcgcgttt gagagcaaca gcgccgtact cacccctaaa
    17221 ggacgagcgg tgatcgacaa gctcattccc gcgctcaagc gctcgcccga atcggccatt
    17281 gaaatcggcg gccataccga ttcctatggc gacccggact acaatctcca gttgagccgg
    17341 acgcgagccg agtcggtccg ccaatatctg gccgatcatg gagtgactaa tcgattgacc
    17401 gccgtcggct atggcgcctc ccgtcccctg agccaggacc ggacgcgcgc cgcctccaaa
    17461 aagaaccggc ggattgaatt tcgtgtgaaa gaggaacggt gaacgtatgg gagccttaat
    17521 tctgcaaatg atcggatgct tactggtggc ggcgaccatc ggg
//
LOCUS       DAOQEW010000068        17062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_882_length_17062_cov_7.511848, whole genome shotgun sequence.
ACCESSION   DAOQEW010000068 DAOQEW010000000
VERSION     DAOQEW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_17062_cov_7.511848"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..1753)
                     /locus_tag="PLS35_09210"
     CDS             complement(332..1753)
                     /locus_tag="PLS35_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNI18882.1"
                     /translation="MKLPSSTPPAPYDGSALIADPIHEYVSFTVPYATPDPSERTEKD
                     LIDSPWVQRLRYIYQLQSARWVYPSAEHTRFVHSLGTMHVAGRFARHLYPFLRNAVPD
                     LPSAAFVEEFLRITALVHDIGHGPFCHFFDDNYLHGFGLSHEKLGQIIVREHLGPLIK
                     KIRRSPSGPFDRGEELNPDHIAHVILKEKGKDNSKIPRWINVLQPVISGSYTADNLDY
                     VLRDAYMCGVAVGPVDLTRLIHYTIVTEKGFTIHKTGLPALHMFLNTRMYLYSNVYYH
                     RTTRAIDIHLRDIFGDTMKLLFPSNPAKHMDEYLTLTDWSLLEDVRRWKKAGQSSLRR
                     LHQEWAHILSRDVKWKMAYSTVLKEKGIERGMDFPSHEQFQQQIQNALPAKLRDLPFR
                     VDMAPLDPRPDPKDTRGIPLLVYDPGTKGLSTEPLEEFLDLLPTRLVQFRIYALDHAH
                     DAALSRAAATVLNKTPSSIETNF"
     gene            complement(1777..2256)
                     /locus_tag="PLS35_09215"
     CDS             complement(1777..2256)
                     /locus_tag="PLS35_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNI18883.1"
                     /translation="MADVRSLFRQILHLDETPHRTALAFAVGVFIGFSPAYGLHMIMV
                     GLCAWLFGWNVVALLAGAFLNNPWTLIPILGATYWTGATLLGMREFPTFDWSDLSFTG
                     LYHQVAPHAWPFVLGGMVLSIAGSLLAYPIAYVLLTRYRRARAVERATHLPPPSGLG"
     gene            complement(2279..3319)
                     /gene="thiL"
                     /locus_tag="PLS35_09220"
     CDS             complement(2279..3319)
                     /gene="thiL"
                     /locus_tag="PLS35_09220"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNI18884.1"
                     /translation="MPPGRTRRASTVHSEFGLIRKLRAQAAQPDPQVFKGIGDDTAIL
                     KTSSTEWTLITTDLLAEGVHFDPTTSSFRDIGYRAAIANLSDIAAMGGTPRFMLVSIA
                     IPRACSTTQIQQLYRGMTEADAPYRVRLVGGDTSASKTGLFLNITLTGVVKPRQALLR
                     SSARIGDLIYVTGTLGDSRAGLDLLTSRRRPKLRPTDARFLLGRHHRPSARIAEGLWL
                     VRHGLAGAAIDLSDGLTGDLRHICEESGVGAEIIAGSLPLSRACRAYAMARGLDPVEI
                     ALQGGEDYELLFTVPRGQQLRFERLVKKTGFRMTCIGSITPKAAGLRLRTDAGATRPL
                     PLTSYEHFRRPT"
     gene            complement(3395..5893)
                     /gene="lon"
                     /locus_tag="PLS35_09225"
     CDS             complement(3395..5893)
                     /gene="lon"
                     /locus_tag="PLS35_09225"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNI18885.1"
                     /translation="MAEAVEQDFSSNQNLEPPDQLPLLPVRDIVVFPYMVLPLFVGRE
                     MSIKAIEAALAGNRMLFLATQKSLDVENPQPEDIHSVGTVGIIMRMLKLPDERIKILV
                     QGLAKGRIEQYIQNDPYYSVKIEKLVETKQPGSTLETEAVMRTVKEQIEKIVSLGKVL
                     IPDVMVVIENLEDPGRLADMVASNLGLKVDITQAVLEIVDPIQRLRQISEILAKEIDV
                     LSMQQKIQAQAKGEMDKTQREYFLREQLKAIQKELGELDERAEEVAEFRKRIKDAKMP
                     EKVLKETEKQLKRLEKMHPDTAESATVRTYLEWMVELPWNKKSKDNLDLKAAMKVLNE
                     DHYDLEKVKERIVEYLAVRKLKEKMKGPILCFVGPPGVGKTSLGKSIARALGREFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRIIQGMKQAGTNNPVFMLDEVDKVGMDFRGDPSA
                     ALLEVLDPEQNSTFTDHYLGVPFDLTEVMFVTTANLMDPILPALRDRMEVIDIPGYTE
                     EEKLGIAQKYLIPRQMNEHGITEKHIRVSETAIRHVISHYTREAGVRNLEREVANLMR
                     KVAKKVAEGKAECHAIDQTNLNKYLGVAKFIPEAELEKDEIGVATGLAWTESGGDVLY
                     IEATVMKGKGQLTLTGHLGDVMKESAQAALSYVRSREKTLGISPDLFAKNDIHIHVPA
                     GATPKDGPSAGITMATAIASALAQIPVRRDLAMTGEITLRGRVLPIGGLKEKILAAKR
                     AKLSTVALPKRNKKDLEEIPKHILKGIELIFVDTVDDVIKAALRRAPSRKATAGAPKT
                     PLKKPSPVTASKRKAAASPRRRRALTGTTRSLSL"
     gene            complement(5980..6906)
                     /gene="galU"
                     /locus_tag="PLS35_09230"
     CDS             complement(5980..6906)
                     /gene="galU"
                     /locus_tag="PLS35_09230"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNI18886.1"
                     /translation="MTRTDVRKAIIPAAGLGTRFLPATKASPKEMLPLVDKPLIQYVV
                     EEAVASGIEDIIVITGRGKRAIEDHFDRSLELEENLKGSGKGQVLNQMRHISNLANFC
                     YVRQPEAMGLGHAVLCAQHLIGDEPFAVILGDEIIDAAVPGLAQLIHIYKQRKGAVLG
                     VQEVPQQEVSRYGIISARKVRSGLHRVEDLVEKPAPADAPSNLAVIGRYVLPPEIFPI
                     LRKTPPGKNGEIQLTDALRQLVKNTPMFAHEVEGQRHDAGDKLGFLIATVELALKNPS
                     LGPAFGDFLQQRLRPTTADVRRRGPGRGRATT"
     gene            7222..9546
                     /gene="bamA"
                     /locus_tag="PLS35_09235"
     CDS             7222..9546
                     /gene="bamA"
                     /locus_tag="PLS35_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNI18887.1"
                     /translation="MTKSGGRRWVPILVLVLAALFVIGEAGWVRAQVSAPLVNSVTIR
                     GQKRIELQAIQGRLTLKANDRFTPDALREQVKILYGTGYFEDVQVETETADGGVAVGF
                     IVREKPFITEIVFDGNEELSDDKLKEKITIKSQTFLDQQQAKESAEKIRLAYQEDGFY
                     NCQVIPVIQAVDEDRKRLTYFIKEGTKAKVRRINFEGMRAVTKEEVFKVTATREWIPW
                     YGLVTQLKLPSLLSDAGVLKREELGNDIERIREVYLNKGYLNVQISQPTLELSDDKKW
                     FEISYSVVEGEPFIVQEVGFRGNTVFEDHELREGLGIRPGEIFQRAKIRGEITRITDL
                     YGAKGYVFADVVPNVTPDNNARTATITLNVKEGEMMRIREIHVTGNDKTRDNVVRREL
                     RLDEQDVIDTLALKRSFQRLNNLNFFETVEILPQQVDVDKVDLNVKVKEKPTGQFSIG
                     GGFSTLDKLVAIADITEGNLGGNGWLGRIRGQLGQQRSLGLVTFRNPYVNDSYNALQL
                     DIYRSMTNYISYFESKSGASATWSRWLSEYVNGSISLFGEQLKYSDPQEGLCPDLIPL
                     ICRQLGTQSSTGFRTSLFRDTRDYYLDPRTGWRLSVGADYGTPALGGSNHFYKVYFDV
                     IKYTPLVYDTRFSVHVRYGQTEGIGGRPIPLTERFFVGGINTMRGFVFGRAGPVTPSG
                     SLLGSAKELIFNNDFIFTISSEAKLNGVFFFDYGNGFDDNEPVQFNKLRSAAGFEARW
                     ISPFGPLRAAYGINLDPRPTERMGVFEFTIGSLF"
     gene            9588..10163
                     /locus_tag="PLS35_09240"
     CDS             9588..10163
                     /locus_tag="PLS35_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNI18888.1"
                     /translation="MRGLVGWKSVRGAGWNGLCLALLAVTLLVGGCRSNPSTVPTSTR
                     IGVVDPQRILGETNAGKRAKDMLASFAKNRQALIELEEKELRRMEEDFVKQGSVLSAT
                     AKREREEQFRRRMAEYQQKVTDLNREVQDKQKEVLDGFRDKIESLSAKVAKRLELQAI
                     FDRGRGGPTLYFDEAVDVSSQLIEEFNKTYP"
     gene            10174..10710
                     /locus_tag="PLS35_09245"
     CDS             10174..10710
                     /locus_tag="PLS35_09245"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNI18889.1"
                     /translation="MNVRNWMAAGVLQVLVAWGAQPVLAADHLKVAMMDQQQVIERSV
                     AGKRALEELKSYSTTRQKIIDSDDQELKDLEKGMQDASMSEDARKEKQEHFRTKLEAY
                     QRRIQDFNREVQEKQRGMVAEYAQKIQAAAAAVAQKEGYTAVLDKGSESTVKIVIYSH
                     PSVDLTEQIIKEFDRQNK"
     gene            10753..11217
                     /gene="fabZ"
                     /locus_tag="PLS35_09250"
     CDS             10753..11217
                     /gene="fabZ"
                     /locus_tag="PLS35_09250"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNI18890.1"
                     /translation="MSVMDNVEIQSILPHRYPFLLVDRIRELDPDRRIVGIKNVTINE
                     PFFQGHFPGRPVMPGVLILEALAQVGGVLAFKSLGAVGRPVVYLTGIDGAKFRKPVVP
                     GDILRLEVDVLKKRAPFWKMQGKAFVDTELVCEAEVTAMVMDEKAAGEKAAG"
     gene            11344..12153
                     /gene="lpxA"
                     /locus_tag="PLS35_09255"
     CDS             11344..12153
                     /gene="lpxA"
                     /locus_tag="PLS35_09255"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNI18891.1"
                     /translation="MKIHPTAVIHPKAVLADDVEVGAYSVVGEHVTIGAGTRVLSHVS
                     IDGWTDIGERCELHPFVSVGGPPQHMQYKGEPTKVVIGHDNILREYVTVNRATVQGGG
                     VTTVGHSNFLMAYVHVAHDCHLGNHLILANAASLAGHISIGDHAIIGGLSGIHQFVRI
                     GAYAMVGGCCALGQDLPPFMRAAGGYRARMYGLNSIGLRRHGFSAERIAALKKAYEVL
                     FRSGHRVAEAVKLAREQFGASQDVLQVADFMQGTKRGICRSVGKDQEGEEE"
     gene            12159..13001
                     /gene="lpxI"
                     /locus_tag="PLS35_09260"
     CDS             12159..13001
                     /gene="lpxI"
                     /locus_tag="PLS35_09260"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNI18892.1"
                     /translation="MGGRAAHDSQAPEGQRIGLIAGNGRFPIIFADNAKRLGYSVFAV
                     AHEGETDPELAQHVDNIHWIKIGQFGKLIDALKGDGVQQAVMLGGIKKTHVFTTVRPD
                     FRALALAAKLIHLKDDDILRRVAEEIEQEGIRICESTFGLQGILVEAGTLTRREPSKK
                     EWEDIRYGWDVGKQIGALDIGQCVVVKDRVIVAVEAVEGTDGAIRRGGELAQGGAVVV
                     KRCKPQQDLRFDLPAVGPRTIEVMEAVKASVLALEAGRGILLDRDDTIAKANRAGIAI
                     VGMT"
     gene            13054..14043
                     /locus_tag="PLS35_09265"
     CDS             13054..14043
                     /locus_tag="PLS35_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNI18893.1"
                     /translation="MTALRAGVIGVGHLGQHHARHYATLPGVTLAGVYDASPDRAKLI
                     AERHGVQAWTNLGEVLTQVDLVSVAAPTSAHFAAAKACLEAGKHVLVEKPIAVTSAEA
                     HELVELAARRGCLLQVGHSERFNPIMQRMRASIERPAFIEGHRLSAFGERGTDVDVVL
                     DLMIHDLDLVLSFQPGPVEEVRAAGVPVLSSNIDIANARIAFASGCVANLTASRVSTN
                     KMRRLRFFQRDRYVSIDFQSRQAMVSRRVQGTGPRPTIDVETFQAGDEEPLRLELESF
                     IHAVATGSRPVVSGEDGEAALNLAGRVLDAIGQFTQRHSAGDVSAAAFGHDSL"
     gene            14083..15216
                     /gene="lpxB"
                     /locus_tag="PLS35_09270"
     CDS             14083..15216
                     /gene="lpxB"
                     /locus_tag="PLS35_09270"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNI18894.1"
                     /translation="MPRILIVTGEASGDLHGAHLVKALKELAPSLQIVGIGGASMRAA
                     GAELVKDIPQLDVMGLIGLSAVKAMLQRISRIRALIKRERWDLVVLIDNPGLNFHFAR
                     VARACRLKVVYYIAPQVWAWRRGRMRWIQQRVDHVLAILPFEEPLYKKAGVRCTFVGN
                     PILDEVASTYDREALRRQFELSDAGPVIGLFPGSRKAELLEHVPLLIETVKRLAQRHP
                     TMQFILAQASSVQDQFLADLLKDSPVPIKVLRNQASEVMAASDLLVVKSGTSTLQAAV
                     VGTPMILFYRAPSWVTYRLARLLIRVPWIGLANLVADRGIVPELIHDEATPERLAQET
                     ERLLADPRAYAEMKAALLSVRQALGTPGASRRAAEAVLAECRS"
     gene            15213..16949
                     /gene="msbA"
                     /locus_tag="PLS35_09275"
     CDS             15213..16949
                     /gene="msbA"
                     /locus_tag="PLS35_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNI18895.1"
                     /translation="MKHYLRLLAYLNPYRFRLGAAFVCALLVAGLSAAYAWLVRPVLD
                     GLFISKDESLLLVLPVAILLVAVLKGVFNYGQNYLMNYVGNQVIGDIREQLFSKLVRL
                     PVHFHDTNTSGRLVSRVINDVNQMANAVAGVLKDLFQQGLTFLAMIGVIIYQNWKLAM
                     VSMIVVPLSVVTMARMGKKLRGLATRGQERMGDMASTLQEALAGIRMVKSFGREEDEA
                     KRFRQSNDAFIHTTMKAIQVSSLGSSHMEVIGVLGVAGIIWYGGYLVIHDEMTPGAFF
                     SFLAAMFMAYTPIRRLSGANNTVQQALAAAERVFDVLDLPTEQVANGGQTELPQISRS
                     LEFRQVAFQYEGQGDAALKAIDLTIRAGEVIALVGSSGSGKTTLVSLLPRFYDPSGGQ
                     ILIDGVDIRNCTLQSVRGQIGIVSQEVVLFDDTVRNNIGYGRQGATLDDVMQAAKLAY
                     AHDFISRMPDGYETLIGERGLKLSGGERQRLAIARAILRDPPILVLDEATSALDTQSE
                     RIVQMALSNLMRNRTTLVVAHRLSTIQNADRIVVLDRGTVAEIGTHDELLRKGGLYKR
                     LHAMQFADALPQ"
BASE COUNT         3395 a         4711 c         5367 g         3589 t
ORIGIN      
        1 tgccccgtgg ggtgaggcag tccaacacaa acatggcgcc gcacaatgca gcaataaacg
       61 cgcctatgcc cagttcgcga atgcgaggcg tcaggttgtc ggtcattagc ccgtagagac
      121 cattgctggt catggggctc ctgctttgtg gcgcaccgtg ccgtcgctca gatagacaga
      181 gagcgataga tcaattgtct agtcagtatg agtaagagaa gtaaaattca atagatgcga
      241 tatgccctag tgcgattcct aggtgtgaga gtaagggccg gaatccgttt tgccgggtgt
      301 gcattgggcg gcggcagcaa ggaacgacgg gtcagaaatt cgtctcgatg ctggacgggg
      361 tcttgttcaa gacggtcgcg gcggctctgg agagggcggc atcatgggca tgatcgagcg
      421 catagatgcg aaactggacc agccgggttg gaagaaggtc gagaaactcc tctaacggct
      481 cggtggagag tcctttggtg ccgggatcgt aaacgagcag aggaatcccg cgcgtatctt
      541 tcgggtccgg tcgtgggtcc aggggggcca tatcaacgcg gaacggcagg tctcgtaact
      601 tcgccggcaa cgcgttctga atctgctgct gaaattgctc gtggctggga aagtccatgc
      661 cccgctcgat gcccttttct ttcagtaccg tgctgtacgc cattttccat ttcacgtcgc
      721 ggctgaggat atgcgcccat tcctggtgca agcgacgcag gctgctctgc ccggcttttt
      781 tccatcgtcg cacgtcttcc agtaacgacc agtcggtgag cgtcaggtac tcgtccatat
      841 gtttggctgg attcgagggg aacaacagct tcatggtatc gccgaagata tcgcggagat
      901 gaatgtcgat agccctggtc gtccggtgat aatacacgtt cgagtataag tacattctcg
      961 tgttcaaaaa catgtgcagg gccggcaggc cggttttatg gatcgtgaag cccttctcgg
     1021 tcacgatggt gtaatgaatc aatcgcgtga ggtcgaccgg tccgaccgcc acgccgcaca
     1081 tgtaggcgtc gcgcagcaca tagtccaaat tgtcggcggt ataacttcct gaaataaccg
     1141 gttggagcac attgatccag cggggaatct ttgaattgtc ctttcccttc tccttcagga
     1201 tgacatgcgc gatgtgatcc ggattgagtt cctcgccgcg atcgaacggc cccgacgggc
     1261 tacgtcgaat ctttttgatg agcggtccga gatgctcccg gacgatgatc tgtccgagct
     1321 tttcatgcga aaggccgaag ccgtgcagat agttgtcgtc gaagaaatgg cagaacgggc
     1381 catgtccgat gtcgtggacg agcgcggtga tgcgcaggaa ttcttcgacg aaggcggcag
     1441 agggaaggtc gggcacggcg ttgcgcaaaa agggatacag gtgtcgcgcg aatcgtccgg
     1501 ccacatgcat ggtgcccaaa gaatgcacga accgcgtatg ctccgccgat ggatacaccc
     1561 agcgggcgct ctggagctga taaatgtaac gcagccgttg aacccaggga gaatcaatca
     1621 ggtctttttc agttcgttct gatgggtctg gtgtcgcata gggcacggtg aacgacacgt
     1681 attcgtgaat cggatcggcg atgagggccg atccgtcgta gggagccggt ggagtggagg
     1741 atgggagttt catagggtca tgaccgacga aggatcttag cccagccctg agggcggggg
     1801 caaatgggtg gcgcgttcaa ccgctctcgc acggcgatat cttgtgagca gcacataggc
     1861 gatggggtac gccagcaggc tgccggcgat gctcaggacc attcccccga gtacaaacgg
     1921 ccaggcatgt ggcgctacct gatggtacag gccggtgaat gacaagtcgc tccagtcgaa
     1981 cgtaggaaat tctcgcatgc cgagcagggt cgcgccggtc cagtacgtcg cgccgaggat
     2041 cggaatgagg gtccagggat tattgaggaa tgctccggcg agcagcgcga cgacgttcca
     2101 gccgaacagc caggcgcaca ggcccaccat gatcatatgc aggccatacg ccggcgaaaa
     2161 gccgatgaag acgccgacgg caaaggccag cgcggtccga tgcggcgttt catcgagatg
     2221 cagaatttgg cgaaacagcg atcgtacgtc ggccataagt cgtcaggtat cgggctcgtt
     2281 acgtcggcct gcgaaaatgt tcgtagctgg tgagtggcag gggtcgtgtg gcgcccgcgt
     2341 ccgtcctcag tcgtagaccg gccgccttcg gcgtgattga accgatacag gtcatacgaa
     2401 atccggtctt cttaaccagt cgttcaaacc ggagctgttg tccgcgaggg acggtaaaca
     2461 gcaattcata atcctcgcct ccctgaagcg cgatctcaac agggtcgagg ccgcgagcca
     2521 tcgcatacgc tcgacaggct cgcgaaagcg gcagtgaacc ggcgatgatt tcggcaccga
     2581 ccccgctttc ctcgcagata tgacgaaggt caccagtcag gccgtctgag agatcgatgg
     2641 cggcccccgc gagcccatgt cgaaccagcc acagtccctc cgcgatgcga gccgacggac
     2701 ggtgatgccg gcccagcaga aaccgagcat ctgtcggtcg cagtttcggc cgccgccgcg
     2761 atgtcaggag atcaaggccg gcacgggagt ctcccagtgt tccggtgaca taaatcagat
     2821 cgccgatgcg tgcgctgcta cgtagtagcg cctggcgtgg tttcacgaca ccggtgagcg
     2881 tgatgttgag aaacagtcct gtcttggagg cagacgtgtc accgccgacg aggcgcaccc
     2941 gatagggagc atcggcctcc gtcatgccgc ggtagagttg ttgaatctgc gtcgtcgagc
     3001 aggcccgcgg gatcgcaatt gagaccagca taaaccgtgg cgtgccgccc attgcggcga
     3061 tatcgctgag attggcgatg gcggcgcggt agccgatatc ccggaaggag gaggtcgtag
     3121 gatcaaaatg caccccctcc gccaggagat ccgtggtgat gagggtccat tcagttgacg
     3181 atgttttgag gatcgcggtg tcgtcgccga tccctttgaa gacttgcgga tccggttggg
     3241 cagcctgagc ccgcagtttt cggatcaggc cgaactcact gtgcacggtg ctggcccggc
     3301 gggttcggcc tggcggcatt actccacctt cttccaggcc gattgccgta ccacatattc
     3361 agggctggca gcagcagtcc tcatgcaacg cgggttacag agacagtgag cgggtggtcc
     3421 ctgtcaacgc acgcctgcgg cgtggcgagg cggctgcttt tcgcttggag gccgtcacgg
     3481 gcgagggttt cttcagcggc gttttcggtg ccccagccgt agctttacgc gagggagccc
     3541 gccgcagggc tgccttgatg acatcgtcca ccgtatcgac gaagatcaat tcaatccctt
     3601 tcaggatatg tttggggatc tcctccaagt ccttcttgtt gcgtttgggc agggcaacgg
     3661 tggaaagttt ggcccgtttg gctgcgagga ttttttcttt caagcctccg atcggcaaca
     3721 cccgcccgcg gagcgtaatt tctccggtca tggcaagatc gcgccgcacg gggatttgcg
     3781 ccagggcgga cgcgatggcc gtggccatcg tgattccggc agaggggccg tctttcggtg
     3841 tcgcgccggc cggcacgtgg atatggatgt cgtttttcgc gaaaaggtcg gggctgatgc
     3901 caagcgtctt ctcgcgcgac cgcacgtaac tgagtgcagc ttgggccgac tccttcatga
     3961 catcgccgag atgtccggtc agcgtgagct gccccttgcc cttcatgacg gtcgcttcga
     4021 tgtagagcac atctccgccg ctctcggtcc aggccagacc ggtagcgacg ccgatttcgt
     4081 ccttctcaag ttccgcctcg ggaatgaatt tcgccacgcc caaatacttg ttcaggttgg
     4141 tctgatcgat ggcatggcat tcagccttgc cttcggccac cttcttggcc accttccgca
     4201 tgaggttggc cacttcacgc tccagattgc ggacgccggc ctcgcgcgtg taatgggaga
     4261 tgacatggcg aatcgccgtc tctgaaacgc ggatatgttt ctcggtaatc ccatgctcgt
     4321 tcatctggcg aggaatgagg tatttctgcg caatcccgag cttttcctct tccgtgtatc
     4381 cgggaatatc gatgacttcc atacgatccc ggagagcagg aagaatcgga tccattaagt
     4441 tggccgtcgt gacgaacatg acttccgtca agtcaaacgg cacgccgaga tagtggtcgg
     4501 tgaacgtgct gttctgctca gggtccagca cttccaggag cgccgccgag gggtcgccac
     4561 gaaaatccat gccgacctta tcgacttcgt cgagcatgaa gaccgggttg ttcgtgcccg
     4621 cttgtttcat gccttgaatg atccgtcccg gcagcgcgcc gacgtaggtc cggcgatggc
     4681 cgcggatttc ggcttcgtcc cgcactcctc ccaggctgat tctgacgaat tcgcggccca
     4741 gcgcccgcgc gatcgatttt cccagcgatg ttttgccgac cccgggaggg cccacgaagc
     4801 acagaatcgg ccccttcatt ttttctttca gcttgcgcac ggccagatac tcgacgatgc
     4861 gctccttcac cttttccaga tcgtagtggt cctcgttcag gaccttcatc gcagccttca
     4921 ggtcgagatt gtccttgctc tttttattcc agggcagctc gaccatccat tccagatagg
     4981 tgcggactgt ggccgattcg gccgtgtccg gatgcatttt ctccagccgc ttgagctgct
     5041 tttcagtctc cttgagcacc ttctcgggca tcttggcgtc tttgatgcgc ttgcggaatt
     5101 cggccacttc ctccgcccgc tcgtccaatt cgcccagttc tttttggatg gctttcagct
     5161 gctcgcgcag gaaatattcc cgctgggtct tgtccatctc gcctttggcc tgcgcctgaa
     5221 ttttctgctg catcgagagg acatcgattt ctttcgcgag gatttcgctg atctgccgta
     5281 gccgctggat cgggtccacg atttccagca ccgcctgggt gatgtcgact ttcagcccca
     5341 ggttggaggc caccatatcg gcgaggcgtc ccggatcctc caggttttcg atcacgacca
     5401 tcacgtccgg aatcaggacc ttgccgaggc tgacgatctt ttcgatctgc tccttgacgg
     5461 tgcgcataac cgcctcggtc tccaaggtgg agccgggctg cttcgtctcg acaagttttt
     5521 caattttgac ggagtagtag gggtcgttct ggatatattg ttcgatccgt cccttggcga
     5581 ggccttgcac caggatcttg atccgctcat cgggcagctt gagcatccgc atgatgatgc
     5641 ccactgttcc caccgaatga atgtcctccg gttgcgggtt ttccacatcc agggattttt
     5701 gtgtcgccag gaacagcatc cggttccccg ccagcgccgc ctcgatcgcc ttgatcgaca
     5761 tttcgcggcc gacgaacagg gggagcacca tgtagggaaa gacgacgatg tctcggacgg
     5821 ggaggagtgg aagctgatcg ggcggttcga ggttctgatt gctcgaaaaa tcttgttcta
     5881 ctgcttcagc catgaggctc cagtctgctg gtgtatggtc tcagtgacga tgtcgtgcga
     5941 gggcacggtt cgggtgacgg tgatccatca tgcccggggc taggttgtcg cgcgccctcg
     6001 acccgggccg cggcgacgga cgtcggcggt agtcggtcgg agtcgctgct gcagaaaatc
     6061 gccgaatgca ggtcccaagg acgggttctt cagggccaat tccaccgtgg cgatgagaaa
     6121 tccgagtttg tcgccggcat catgccgctg tccttcgact tcgtgcgcga acatcggcgt
     6181 gttcttcacg agttgccgca gggcatccgt caattggatt tctccgttct tgcccggagg
     6241 ggtcttgcgg aggatcggga aaatttcagg tggcagcaca taccgtccga tgacggccag
     6301 attcgacggg gcatcggcgg gcgcaggttt ttcaacgagg tcttccaccc ggtgcagacc
     6361 gctgcgcacc ttgcgggccg agatgatgcc gtaccggctg acttcctgct gcggcacttc
     6421 ctgcacgccg agcacggcgc ctttgcgctg cttgtagata tggatcaatt gcgcgagacc
     6481 gggaaccgcc gcatcgatga tctcgtcacc caggatgacg gcgaacggtt catcgccgat
     6541 cagatgctgc gcgcacaaga cggcatgacc cagccccatg gcttccggct ggcggacgta
     6601 gcagaagttc gccagattgg aaatgtgccg catctggttg aggacctgcc ctttgccgct
     6661 gcccttgaga ttttcttcga gttcaaggga acgatcgaag tgatcttcga tcgcgcgctt
     6721 gcctcgcccc gtgatcacga tgatgtcttc gatgcccgac gcgaccgctt cttcgacgac
     6781 gtactgaatc agcggtttgt cgaccagcgg cagcatctct ttcggggagg ctttggtggc
     6841 gggaaggaag cgtgtgccca gtccggcagc gggaataatc gctttacgta cgtctgttct
     6901 tgtcataacg gcgatagtat gtgatggggt acatgaagtc aagatttcgt cggctctttt
     6961 cgatcgctgc cggcgcgccg ccgcgcaggg aaactccttt acattgaaaa atgtgctcaa
     7021 tataatccga gagtttttct gcagacgtgc ggtggtgctg caacgaacgc ggcgcatcgg
     7081 tgactcttat gacgacagtt cgcgtccctt cgcccgacca tcggttcgcg gcaagacacg
     7141 cggcatggca tgtgacgatc tctcatcctg aaggtcgaac gaaccagtgc ttgctctccc
     7201 agacggtacg gaaggattac ggtgaccaag tcgggaggca ggcggtgggt gccgattctc
     7261 gtccttgtcc tggcggcgct gttcgtgatc ggcgaagcag ggtgggtgcg ggcccaagtc
     7321 agtgccccgc tggtcaattc cgtcaccatc cgtggtcaga aacgcattga actgcaggcg
     7381 atccaggggc gtctcaccct caaggccaac gatcggttta ctcctgacgc gctccgggaa
     7441 caggtcaaga ttctctacgg aaccggctac ttcgaggatg tgcaggtgga aacggagacg
     7501 gccgatggcg gcgtcgccgt cggattcatc gtccgtgaga aacccttcat taccgagatt
     7561 gtgttcgatg gcaacgagga attgagcgac gacaagctca aggaaaaaat cacgatcaaa
     7621 agccaaacct ttctcgacca gcagcaggcg aaagagagcg cagagaagat ccgcctcgcg
     7681 taccaggaag acggcttcta caattgccag gtcattccgg tgattcaagc cgtcgatgag
     7741 gatcgcaaac ggctgaccta tttcatcaag gaaggcacca aagcgaaagt ccgacggatc
     7801 aatttcgaag gcatgcgcgc ggtgaccaaa gaagaagtct ttaaagtgac ggccacgcgc
     7861 gagtggattc cctggtacgg cctggtgacg caactgaaac tgccgtcgct gctgtcggat
     7921 gccggtgtct tgaagcgcga ggagttgggc aacgatatcg aacgtatccg cgaagtctat
     7981 ttgaacaaag gctacttgaa cgtgcagatc agccagccga cgctggaact gagcgacgac
     8041 aagaagtggt tcgaaatcag ctattcggtc gtcgaagggg agccgttcat cgtgcaggaa
     8101 gtcgggttcc gcggcaacac ggtcttcgag gatcatgaat tgcgtgaagg tttgggaatc
     8161 cgcccgggcg aaatctttca acgggccaag atccgcggcg aaatcacgcg cattaccgat
     8221 ctctatggcg cgaaggggta tgtctttgcc gatgtcgtgc ccaacgtgac gccggacaac
     8281 aatgcgcgca ccgcgacgat caccctcaac gtcaaagagg gcgagatgat gcggatccgc
     8341 gagatccatg tcaccggcaa cgacaaaacg cgcgacaatg tcgtacgccg ggaactgcgc
     8401 ctggatgagc aggacgtcat cgatacgctg gccttgaaac gcagctttca gcgattgaac
     8461 aacctcaact ttttcgaaac ggtggaaatt cttcctcagc aggttgatgt cgacaaggtc
     8521 gatctcaatg tgaaggtcaa agaaaaacct accggccagt tcagcatcgg cggcggattc
     8581 agcactctcg ataaactcgt cgcgattgcg gacatcacgg aaggcaacct tggtgggaac
     8641 ggatggttgg gtcgtatccg cgggcaattg ggtcagcagc ggtccttggg actcgtgacg
     8701 ttccggaatc cctacgtgaa cgattcgtac aatgcccttc agctcgatat ttaccggtcc
     8761 atgaccaact atatttccta cttcgagtcc aagtccggag ccagcgccac gtggagccga
     8821 tggctgtcgg agtatgtcaa tggaagcatc agtctcttcg gcgagcagct caagtacagc
     8881 gatccgcaag aagggttgtg cccggatctg attccgctga tctgccgcca gctcgggacg
     8941 caatcgtcca ccgggttccg tacgtcgttg ttccgtgaca cgcgtgacta ttatctcgat
     9001 cctcgcaccg gatggcgctt gagcgtcggt gccgactacg gaacacccgc gctcggagga
     9061 tcgaaccatt tctataaagt gtatttcgac gtcatcaagt acaccccgct ggtctatgac
     9121 acgcggttct cggtacatgt ccgatacggg cagacagagg ggatcggggg ccggcccatt
     9181 cccctcaccg agcggttctt cgtcggaggc atcaatacga tgcgcggatt cgtgttcggc
     9241 cgtgcaggtc ccgtgacgcc atcgggctcg ctcctgggtt ccgccaagga gctcattttt
     9301 aacaacgact ttatctttac gatttcgtcc gaggcgaagt tgaacggggt gttcttcttc
     9361 gattacggaa acggattcga cgacaacgag ccggtgcagt ttaataagtt gcggagtgcg
     9421 gcgggatttg aagccaggtg gatttctccg ttcgggccct tgcgcgcggc gtacggaatc
     9481 aacctcgatc cccggccgac cgagcgtatg ggcgtctttg agtttacgat cggatcgttg
     9541 ttctgatgca ggttctgtgg cgcgggtgta ggaaaggaat tgagcgcatg cgtggactgg
     9601 taggctggaa aagcgtgcga ggagccggat ggaatggact ctgcctggcg cttctggccg
     9661 tcacgctgct ggtcggcgga tgccgctcga atccctcgac ggtaccgacg tccacacgca
     9721 tcggtgtggt ggatcctcag cgtattctgg gcgaaaccaa cgccgggaaa cgagcgaagg
     9781 acatgttggc ctccttcgcc aagaatcggc aggcgctgat cgagctcgaa gaaaaagaac
     9841 ttcgccggat ggaagaagac ttcgtcaagc aaggcagcgt gctcagcgcg actgccaaac
     9901 gtgaacggga agagcagttc cggcgccgga tggctgaata tcagcagaaa gtcactgatc
     9961 tgaaccgcga agtgcaggac aagcagaagg aagtcttgga cggattccgg gataagatcg
    10021 aatccctgtc ggccaaggta gccaagcgat tggaactgca ggctatcttc gaccgcgggc
    10081 gcggaggacc gaccctttat tttgatgagg cggtggatgt gtcgtcgcag ttgatcgagg
    10141 agttcaacaa aacgtatcca tgagagggaa tccatgaacg tacgtaactg gatggcggcc
    10201 ggtgttctac aagtccttgt ggcctggggg gcgcagccgg tcttggctgc cgatcacctc
    10261 aaggtggcca tgatggatca gcaacaggtc atcgagcgca gcgtcgccgg gaaacgcgcc
    10321 ttagaggaac tgaagagtta ttccacgacg cgccagaaga tcatcgattc cgacgaccag
    10381 gaattgaagg atctggaaaa gggcatgcag gacgcctcca tgtcggagga tgcgcgcaag
    10441 gaaaagcaag agcacttcag gacgaaactg gaggcctacc agcgccgcat tcaggacttc
    10501 aatcgcgagg tgcaggaaaa gcagcgcggc atggtggcgg agtatgccca gaagattcaa
    10561 gccgctgcag cggcggtcgc gcagaaggaa ggctatacgg ctgtcctgga caagggcagc
    10621 gagtcgacgg tgaagatcgt gatctacagt cacccgtcgg tcgatctgac ggaacagatc
    10681 atcaaggaat tcgatcggca gaacaagtaa ccgtcgatcg taatcgtcta cgcaagacag
    10741 gaggcggggc tgatgtcagt gatggacaac gttgagattc aatcgatcct tccccaccgg
    10801 tatccgtttt tgttggtcga ccgcattcgc gaactcgatc ctgatcgccg gattgtgggt
    10861 atcaaaaatg tcacgatcaa tgagccgttt tttcaggggc acttcccggg gcgtccggtc
    10921 atgccgggcg tgctgatcct cgaagcgtta gcccaggtcg gcggcgtgct tgcgttcaaa
    10981 tcgctcggtg cagtggggcg accggtcgtg tatttgaccg gaatcgacgg ggcgaagttc
    11041 cgcaagcctg tcgtgcccgg cgatattctg cgattggagg tcgacgtgct caagaaacgt
    11101 gctccattct ggaagatgca gggcaaagcc tttgtcgata ccgagttggt ctgcgaagcg
    11161 gaagtcacgg ccatggtgat ggacgaaaag gcggccggcg agaaggccgc ggggtagagg
    11221 cgcagtttcc ccacccaccg gcggggagtg tagggaacca caggctgtgt acacgctgtt
    11281 cagcgagatt atgcgcatgc tgaccggtgt ttcggcggcc tgccagaggg cgaggaggtg
    11341 tgagtgaaga ttcatccgac cgcggtgatc catccgaaag cggtgctcgc agatgacgtg
    11401 gaagtcgggg cgtattccgt cgtcggtgaa cacgtcacga tcggtgccgg tacgcgtgtg
    11461 ttgtctcatg tgtcgatcga cgggtggacc gatatcggcg agcggtgcga actgcatccc
    11521 ttcgtatcgg tgggcgggcc gcctcaacac atgcagtaca aaggcgagcc gacgaaggtc
    11581 gtgatcggtc acgataacat tctgcgggag tatgtgacgg tcaatcgggc tactgttcag
    11641 gggggcggtg tcaccaccgt gggccattcc aatttcttga tggcctatgt ccatgtcgcg
    11701 cacgattgcc atctcggtaa tcatctcatt ctggcgaatg cggcgagtct ggccgggcac
    11761 atttccattg gcgaccatgc cattatcggc gggctctccg gcattcacca attcgtacgt
    11821 atcggtgcct acgccatggt tgggggttgt tgcgcgctcg gccaggacct cccgccgttc
    11881 atgcgagcgg ccggtggcta ccgcgcccgc atgtatggat tgaactcgat cgggctgcgc
    11941 cggcatggat tctcggccga acggattgcg gcgttgaaga aggcttatga ggtactgttt
    12001 cgctccgggc atcgtgtggc ggaagcggtc aaactggcgc gggagcagtt cggtgcgagc
    12061 caggatgttc tgcaagtcgc cgacttcatg caagggacga aacggggcat ttgccggtcg
    12121 gtgggcaaag atcaggaagg cgaagaggaa taacaagggt gggcggacgt gcagcgcatg
    12181 acagtcaggc gcccgagggc cagcggatcg gactgatcgc gggcaacggc cggtttccga
    12241 tcatctttgc cgacaatgcg aaacgcctcg ggtactccgt ctttgcggtg gcgcatgaag
    12301 gggaaaccga tccggaattg gcccagcacg tcgacaacat ccattggatc aagatcgggc
    12361 aattcggcaa gttgatcgac gcgctcaagg gcgacggcgt ccagcaggcc gtcatgttgg
    12421 gcggcatcaa gaaaacgcac gtctttacca cggtccgtcc ggacttccgc gcgttggcgc
    12481 tggcggccaa attgattcat ctgaaggacg acgacattct tcgccgcgtt gccgaggaaa
    12541 tcgaacagga aggcattcga atctgcgaat caaccttcgg cctgcaaggg attctcgtcg
    12601 aagcgggcac gttgacacgg cgcgagccga gtaaaaagga atgggaagac atccgttacg
    12661 gctgggatgt cggcaagcag atcggcgcgt tggatatcgg ccagtgcgtc gtggtgaagg
    12721 atcgtgtgat cgtcgccgtc gaagccgtgg agggcacgga cggagcgatt cgtcgaggtg
    12781 gtgaattggc acagggggga gcggtggtcg tcaagcgctg caaacctcag caggatctcc
    12841 gcttcgatct tcccgccgtg ggcccgcgca cgatcgaagt gatggaagcc gtcaaagcct
    12901 ccgtgctggc actggaagcc ggacgcggca ttctgctgga tcgcgacgac acgattgcga
    12961 aggcgaaccg cgcagggatc gctatcgtcg ggatgacatg aaggcacacg cgatggatgc
    13021 agaagccggc ctcgtcagac aggagaaggc acgatgacgg cgcttcgtgc cggggtgatc
    13081 ggcgtcggcc atctcgggca gcaccatgcg aggcattatg caacgctccc cggcgtcacg
    13141 ctggccggtg tgtatgatgc gtcgccggac cgcgccaagc tgatcgccga gcgtcatggc
    13201 gtgcaggcct ggacgaatct cggcgaggtg ctcacgcagg tcgacctcgt cagcgttgca
    13261 gccccgacct ctgcccattt cgcggcggcc aaggcctgcc tggaagccgg caaacatgtc
    13321 ctggtggaaa agccgattgc cgtgacctcc gccgaggccc acgaactcgt cgaattggcc
    13381 gcccggcgcg gatgcctgct gcaggtggga catagtgagc gattcaaccc gatcatgcag
    13441 cgcatgcgtg cgtctatcga acgtccggcc ttcatcgaag gccatcggct gagcgcgttc
    13501 ggcgaacggg gcaccgacgt cgatgtggtg ctcgatctca tgattcacga tctggatctc
    13561 gtgctgtcgt ttcagcccgg tccggtggag gaagtccgcg ccgccggcgt gcccgtgctc
    13621 tcctccaata tcgatatcgc caatgcccgt atcgcgtttg cgagcggatg cgtggcgaat
    13681 ctgacggcga gccgcgtctc cacgaacaag atgcgccggt tgcgattctt tcaacgcgac
    13741 cgttatgtct cgatcgattt tcaatcccgt caggcgatgg tctcgcggcg ggtgcagggc
    13801 actgggccga ggccgacgat cgatgtggaa acgttccagg ccggggacga ggagccgttg
    13861 cggctggaac tggaatcctt catccatgcc gtggccacgg gatcgcgtcc tgtcgtgtct
    13921 ggtgaggacg gcgaggccgc gctcaacctg gctggccggg tgttggacgc cattggtcaa
    13981 ttcacgcagc gccattccgc cggcgacgtc tctgcggcgg cattcggcca tgacagtctc
    14041 taggggggcc acgtcgatgt tggtccgcct gagcagagcg acatgccacg tatcttaatc
    14101 gtgaccggag aggcctccgg cgatctccac ggagcccatt tggtcaaggc attgaaggag
    14161 ctggctccgt cgctccagat cgtgggaatc gggggtgcct ccatgcgggc ggccggcgcg
    14221 gagctggtga aggatattcc tcaattggat gtcatgggac tcatcggtct gtctgcggtg
    14281 aaggccatgc tgcagaggat ttcccgcatc cgcgcattga tcaaacggga gcgttgggat
    14341 ctcgtggtcc tgatcgacaa tcccggactg aacttccatt ttgcccgggt cgcccgagcc
    14401 tgccgcctga aggttgtcta ctacattgcg ccccaagtgt gggcctggcg gcgtggccgg
    14461 atgcggtgga ttcagcagcg ggtcgaccat gtccttgcga ttctgccgtt tgaggaaccg
    14521 ctctataaga aggccggcgt gcgctgcacc tttgtcggta atccgattct cgacgaagtg
    14581 gcctcaacgt acgaccggga ggcgttacgc cgccaattcg agctgtcgga tgcggggccg
    14641 gttatcgggc tctttcctgg cagccgcaaa gccgaattgc tggaacatgt ccccctgctg
    14701 atcgagacgg tgaagcggct cgcgcaacgc caccccacca tgcaatttat tctggcgcaa
    14761 gcgtcatcgg tgcaggacca gtttttggcg gatctcttga aggacagtcc ggttcccatc
    14821 aaagtcttgc gtaaccaggc aagcgaagtg atggctgcgt cggatctgct cgtcgtcaaa
    14881 tcgggaacct ctacattgca ggcagcggtg gtgggaacgc cgatgatttt gttttatcgt
    14941 gccccctcct gggtgacgta tcgattggcg cgcctgttga tccgggtgcc ctggatcggg
    15001 ctggccaatc tggtcgcaga ccggggaatc gtccctgagc tgattcatga cgaagctaca
    15061 ccggaacgct tggctcaaga gaccgaacgg ctgctggcgg atcctcgcgc ctatgcggag
    15121 atgaaggccg ccctgttgtc tgtgcggcag gcgttaggca cgccgggggc ctcccgacga
    15181 gctgccgaag ccgtgctcgc tgagtgccga tcatgaaaca ctatctgcgc ctcctcgcat
    15241 atctgaaccc ctatcgattc cgcttgggcg ccgcattcgt gtgcgcgctg ctcgtggccg
    15301 gcttgtccgc cgcttatgcc tggctggtac gtcccgtgct cgatggcctg ttcatcagca
    15361 aagatgaaag cctgctcctc gtcctcccgg tggcaatttt gctcgtggcg gtgctcaagg
    15421 gcgtgttcaa ctatggtcaa aactatctga tgaattatgt cggcaatcag gtgatcggcg
    15481 acatcagaga gcaactcttc tccaaattgg ttcgcttgcc tgtccacttt cacgatacaa
    15541 atacgtcagg acggctggtc tcgcgggtca tcaacgatgt gaatcagatg gcaaatgccg
    15601 tcgccggggt gttgaaggat ctgtttcagc aggggctgac gtttctggcg atgatcgggg
    15661 tgatcatcta tcagaactgg aagttggcca tggtgtccat gatcgtggtg ccgctgtccg
    15721 tggtcacgat ggcgcggatg ggcaaaaagt tgcgaggact cgccacacgc ggacaggaac
    15781 gtatgggaga catggcgtcg actcttcagg aagccctggc cggtatccgg atggtcaaat
    15841 ccttcggccg ggaggaagac gaggccaagc gttttcgtca gagcaacgac gcgttcatcc
    15901 ataccacaat gaaagcgatt caggtttctt ccctgggttc ctcccatatg gaagtgatcg
    15961 gcgtgctcgg ggtggcgggg atcatctggt acggcggcta tctggtgatt cacgatgaaa
    16021 tgactcccgg cgccttcttt tccttcctgg ccgccatgtt catggcctac acgcccatcc
    16081 gacgattgtc aggcgcgaac aacacggtgc agcaggccct cgcggctgca gagcgcgtct
    16141 ttgacgtgct ggatctgccg acggaacaag tggccaatgg cgggcagacg gagttgccgc
    16201 aaatttcccg ttcgctcgaa ttccggcagg tcgcctttca gtatgaaggg cagggggatg
    16261 cggccctcaa agccatcgat ttgacgatac gggcgggcga ggtcattgcg ctcgtgggca
    16321 gcagcggcag tggaaagacc acgttggtga gtttgctccc gagattctac gatcccagtg
    16381 gcgggcagat tctgatcgac ggtgtggaca tccggaactg cacactgcaa tcggtgcgtg
    16441 gacaaattgg aatcgtgtcg caggaagtgg tgctgttcga cgatacagtg cgcaataaca
    16501 tcgggtatgg gcggcagggg gccacgctcg acgacgtcat gcaggccgcg aagctggcct
    16561 acgcacatga ctttatcagc cgcatgccgg acggctacga gacgttgatc ggagagcgcg
    16621 ggctcaaatt atccggcggc gagcgccagc gcctggcgat tgccagggcg attctgcgcg
    16681 accctcccat tttggtgctg gatgaggcaa cgtcggcgtt ggatacgcaa tcggagcgca
    16741 tcgtgcagat ggccctatcc aacctgatgc ggaaccgaac gaccctggtg gtcgcgcacc
    16801 ggctgtcgac cattcagaat gccgatcgta ttgtggtgct cgaccgcggg acggtcgcgg
    16861 aaatcgggac gcatgacgaa ttgctgcgca aaggcggcct gtataagcgc ctgcatgcga
    16921 tgcagtttgc cgatgcgctg ccgcagtaat cgaaggatcg agggtgacgt ggtgagcgac
    16981 tccatgacga acaggtctcc ggcagccccg cagccgaaac aggccctgac gaagcgtctc
    17041 accaatgcac tcaagctcgc gg
//
LOCUS       DAOQEW010000069        16858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_900_length_16858_cov_8.575611, whole genome shotgun sequence.
ACCESSION   DAOQEW010000069 DAOQEW010000000
VERSION     DAOQEW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_900_length_16858_cov_8.575611"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..630)
                     /locus_tag="PLS35_09280"
     CDS             complement(271..630)
                     /locus_tag="PLS35_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNI18896.1"
                     /translation="MVHIKRIYSEPDPQDGIRILVDRIWPRGCTKERARLNDWQKELA
                     PSTALRTWFGHDPRKWKEFRSRYLAELAQPAQRRAIEELVALARSQTLTLLFGSSDVK
                     HNQAVVLKELIDRAERE"
     gene            955..1224
                     /locus_tag="PLS35_09285"
     CDS             955..1224
                     /locus_tag="PLS35_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18897.1"
                     /translation="MIRQSGFPSDDVIDNQILVLLHRHRRLTFLTLADALPLYTWQSL
                     FAALNRLRTQEQVELLPLAADYEVIWRSGRETEPLLSKGGHLQVR"
     gene            1221..1586
                     /locus_tag="PLS35_09290"
     CDS             1221..1586
                     /locus_tag="PLS35_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18898.1"
                     /translation="MMRVSCPAVVRCLVLVAACQMPACGNTASVFDLETNDPRQDQEK
                     IAEFYAHEATRLRQMAQDYDHRVAVYERLFGPHSDWVEGTRLLSQSYEAAAQEQERIA
                     ARHQRLHDRRSHAADQESR"
     gene            1615..2520
                     /locus_tag="PLS35_09295"
     CDS             1615..2520
                     /locus_tag="PLS35_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNI18899.1"
                     /translation="MWRYTTIGLIIAGLCGLSGCGNTVSPGQRGLRWYPLTEGLTTET
                     LKSGFYWRAPWNDVYVYDVRLQSYTELVDALSSDDLLVKLKAAIIMRPIAEEVYFLAQ
                     EIGPDFYPRVVRPELLAAVRSVVSNYPMVAVPEMSAEIASKVQAVVVEKLKGRHLEVH
                     SVALADIELAKIVLEAVERKQAKEQEKEQKEFELVIAEKEAEIARRRARGEGDAIRIR
                     SEGEAEGLKIRALGQANAQQTITKTLTPEYLQYKLYDSPNAKMVLLPDQLRVPILINP
                     DQDRGTRSTPESLMRTEQELMGRGR"
     gene            2558..2791
                     /locus_tag="PLS35_09300"
     CDS             2558..2791
                     /locus_tag="PLS35_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18900.1"
                     /translation="MACSRCQGLMVDETLFNPSEGVSHTWVPVVRCLNCGNLEDALIR
                     LARGISGRLGRRTRPGPQRRGVWSEEIRGRQKE"
     gene            2862..3275
                     /locus_tag="PLS35_09305"
     CDS             2862..3275
                     /locus_tag="PLS35_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI18901.1"
                     /translation="MEGYGKRVLIADDEESVRRLVAIVLEQAGYIVHAAVDGVEGLGE
                     MKKRRFDAVISDFHMPRLDGEQFLLLCRLMWPDIPVVLLSAELLELPAALKQQGACIL
                     VSKPFSPQMLLQALHESLSLPESEQASDRITSKAS"
     assembly_gap    3714..3813
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3814..4159
                     /locus_tag="PLS35_09310"
     CDS             <3814..4159
                     /locus_tag="PLS35_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18902.1"
                     /translation="RWSQRGTGLRNPHARRIVEGGETMNDGTDRNDGYDVDSAVWAYL
                     QRCRICSMEELFRHVPTFTANQLFLTVDRLSREGKVVLRYRNRAEYVMVRTGIADEWP
                     HEMSGAPFGDRT"
     gene            4171..4416
                     /locus_tag="PLS35_09315"
     CDS             4171..4416
                     /locus_tag="PLS35_09315"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNI18903.1"
                     /translation="MKKKTAPKKAATRTTKQVAKPAVSEKQPGPVQDIQVRIAARAHE
                     LYEQRGCLDGYHLRDWLEAEREILGHPVEMDKSSERI"
     gene            complement(4501..6321)
                     /locus_tag="PLS35_09320"
     CDS             complement(4501..6321)
                     /locus_tag="PLS35_09320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNI18904.1"
                     /translation="MAVSRKTASATVSSRRSTTSREFADRWDLADLVADPVKQFDRYL
                     KDLTAKVTRFEAARPDLSATMSEQSFLNLLTLSEQIARDSGRLGAYSYLWFSEDTKQL
                     QARSFKTKVEEHLTSLQNRLLFFDLWWQSVDEKNAERLMANSGDFRYHLETIRRFKPH
                     TLSEPEEKIINIKNITGQSAVHQLYDVVTNGFTFTLRIGGKNKTMNREALTAYLRSPK
                     AGMREAAYREMYRVYESQQDLLGEIYKTLINDWKAENLQLRHFKTPLASRNLSNDIPD
                     SAVEALLAVCMKNAPIFQRYFKLKAKLCKISTMNRYHIYAPHRAEQKSYRYPDAVRMV
                     LDAYYGFSPRLAELAQRVFADRHIDARMKAGKMGGAYCYSVTPNVTPYVMLNFTGEAR
                     DIATMAHELGHAVHAMMAEQHNVFTFHSTLPLAETASVFGERILSDALMASESSKSVK
                     QSLLVNQLDDIYATVMRQAYFVAFERTAHDMVAQGATTNDLAGTYMTLLKQQFGKSLR
                     VPQEFQWEWLTIPHLYASPFYCYAYSFGNLLVLALYRMYQEQGAAFVPKYLDLLAAGG
                     SKAPQAILAQVGVDMNSHAFWQSGFDAISGMVDQLEETMR"
     gene            6476..6805
                     /locus_tag="PLS35_09325"
     CDS             6476..6805
                     /locus_tag="PLS35_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18905.1"
                     /translation="MTEQEINRAIQYVTAATSYGRDTVAEILRTGLSELSQLSTQSTR
                     RFEREDLMGYLCQWTMKRTGHPEPLVREVLDGAGRWLDEVATTLAEQREAEANAGDDD
                     ETGAASA"
     gene            complement(6877..7197)
                     /locus_tag="PLS35_09330"
     CDS             complement(6877..7197)
                     /locus_tag="PLS35_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI18906.1"
                     /translation="MPNPRIEPLKRVLAMEPDDDVAWFGLGKAYMEDANFEEAAKALR
                     QCITVKPTYSAAYYALAQSLLKLNRVEECRQVSDQGIDVSTKNGDAMVTKNLELLKSS
                     LPAA"
     gene            7327..8271
                     /locus_tag="PLS35_09335"
     CDS             7327..8271
                     /locus_tag="PLS35_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNI18907.1"
                     /translation="MGRTGLVYDPRYLAHDMGAGHPESPNRLRAIMQRLEQSGLAAEL
                     TRLAPRTAEDEWITLVHRPEYVGHLNREAPTSGRVSLDADTSMSPGSLLAAYLAAGGA
                     LSGVDAIMADQVQHVFCAVRPPGHHAEAGRAMGFCLFNNVAIAARYAQKRYGVQRVLI
                     VDWDVHHGNGTQHSFEADPSVLFFSTHQYPHYPGTGRATECGTGAGEGFTINVPMEAG
                     EGDDDYRAVFQKVLVPAADAFKPELVIISAGFDAHRDDPLASMGLTEEGYADLTSIVA
                     GIATRHCRGRLLSSLEGGYNLTALAASVERHIRALVAS"
     gene            8268..9542
                     /locus_tag="PLS35_09340"
     CDS             8268..9542
                     /locus_tag="PLS35_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18908.1"
                     /translation="MKWWLKLLLGLAVISGLVYLYYTEVKPVVIFGLRSEYAHAIPFQ
                     KIPEGLTSLKAESCGQCHREIYEEWKTSIHAHAYEDPFFQAYWKKDKQVWVCLNCHTP
                     LENQQPTLVKDIPRGRVEKATQEPNPHFDAALQKESITCAACHVRDGVILGPFDDSAA
                     PHPTKFDPTFRTAQFCSRCHNVVSGPAQFYNVGPCGTYAEYEGKYFMQERGFICQSCH
                     MPEVDRPVAENGPIRRGRRHLWRGGHDPDMVKRAAAIQVKVDPPKPKPGDQVTIALTL
                     TNAGAGHKLPTGDPDRHFTVEFTVKDSNQQILAEKSHTMGRWIMWQPAIVELYDNRLL
                     PLASREYQFAYRMPEASKGVTLKARVRYHILTDGQHEMLKKKYGLMADDPYAFTVYER
                     EVPLNEQLAAAFADPLPEPPPMACANPSAPQG"
     gene            10277..11170
                     /locus_tag="PLS35_09345"
     CDS             10277..11170
                     /locus_tag="PLS35_09345"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNI18909.1"
                     /translation="MQHPLLIDCETLQTRLGQPGLVILDVRGRSTYEFGGHIPGAVHS
                     TWHEYSDPEAIAKGLLDPDVKRIEKRVQALGINRDNEVVIYSNPFDNWGDEGRMFWML
                     EYLGHKNIKVLDGGWVKWIHERRPFEHGAVRPTPGNFTVSPVASAIIMKDELKGIVRK
                     PHPTTTIVDARSLEEFLGKEVSGIPRPGHIPGAVHVAWSGFLNADATVKDLDAIHAQL
                     ELKGLNPAHETVCYCTGGVRSGWLYFVLRLAGFERLRNYPGSWWEWSRDFACPVEKDL
                     LALQKLLGLSESQVPTGMRPS"
     gene            11251..11607
                     /gene="smbP"
                     /locus_tag="PLS35_09350"
     CDS             11251..11607
                     /gene="smbP"
                     /locus_tag="PLS35_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HNI18910.1"
                     /translation="MQTAMWRGVVVAGVMMALVGAPMVSSVAFAAGNKHQAEAVEHAK
                     EAVAHGKQGHADALVKHAEAALKHAEGAMAETKNPHVGEAIKGLKDGIEHGKAGHADV
                     ATKAVENALPHLSEGM"
     gene            11689..12483
                     /locus_tag="PLS35_09355"
     CDS             11689..12483
                     /locus_tag="PLS35_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNI18911.1"
                     /translation="MMRVGYLQGDPVFGEVAHNLDTVTAQLEHAEADLLVLPELFATG
                     YQFTSQEEVSRLAEAVPDGPTTQRLLEIAARRKTTIVAGLAERAGTKYFNSAVVVGPK
                     GFIGCYRKTHLFFEETLFFNPGDSGFQVWDIGAAKIGVMICFDWYYPESARTLALLAA
                     EIIAHPSNLVLPNCPDSMVTRCLENRVFSVTANRIGSEARGGKDRLTFIGLSEIVSPR
                     GQILHRAPRDREELTIIEIDPAEARRKALNSYNDLLRDRRPAMYGV"
     gene            12548..13108
                     /locus_tag="PLS35_09360"
     CDS             12548..13108
                     /locus_tag="PLS35_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNI18912.1"
                     /translation="MTTPLTKGVLLVATPALNDPNFRQAVVLLCEHGPEGALGVIVNR
                     PTAMSISEALPQVPILEGQPHVLYSGGPVQTNQVMMLYRISDTPENSHQVFDGVCLGG
                     DLEIMERILMEQPGKESFRAYLGYSGWGPGQLESEMQTGSWITLPADPSIVFEKEAAR
                     IWSDIVLSLDETSRHYADMPFDPSSN"
     gene            complement(13204..16008)
                     /gene="uvrA"
                     /locus_tag="PLS35_09365"
     CDS             complement(13204..16008)
                     /gene="uvrA"
                     /locus_tag="PLS35_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNI18913.1"
                     /translation="MASRTPPTRSTDELIIEGARQNNLKNISLRIPHNKVTAITGLSG
                     SGKSSLAFDTLFAEGQWRYVESLSTYARMFLDKVNRPDVDRITNIRPAIAIEQKNPIR
                     TARSTVGTATEVADLLRLLFAKIGKPVCPDCHQEARGYHPGSLAEELLAQFPDARAMI
                     LFPVNDLGPGHDRSLLDSLTKRGFTRLRCGDEILDLHDNVTLPEHRESGLQVVLDRLV
                     LRPDNRHRLIEAIEVAFQEADGVCQVDVIGHGLRTYSTHFRCQGCGRTFEPLRPLLFS
                     FNHPLGACPECKGFGNILRYDRDLVIPDRSKSLAGGVIEPWSKPGSDWWQKQLLLAMK
                     KLEVDLTAPFQELPSEVQQLIWEGSDQVEGVRQYFDYLETKRYKLHVRVLLSRYRSPA
                     TCPICQGSRLKPAARFVKLAGHDFIQLNDFTIQAATEWFQRLALPAFDAEIAKDILRQ
                     LQAKLNFLLRVGLNYLTLSRQTKTLSGGEAQRIALANQLGSRLVGTLYVLDEPTIGLH
                     ARDTDTLAGILRDLATEGNTVVVVEHDPLMIQAADHIVEMGPASGEQGGQVVCAAPRA
                     PFIRDPKSLTARYLRGEERIPLPKTRRSGNGKVLSIAGAAEHNLKNLLVRIPLHMLVC
                     ITGVSGSGKSTLIEDTVYRAAARAFRIESLPMGRFQAIKGLEYLKGVRLIDQQPIGRT
                     PRSNPITYLKAFDEIRQLFASERDALRQGLTPGHFSFNAAGGRCERCEGNGYEKLEMY
                     FFEDIYATCEQCNGRRFKPEILAIKYRGKTIHDVLNLTVTEAQAFFSGSPKLTEKLYL
                     LSSIGLGYLRLGQAANTLSGGEAQRLKIAAELKDPSAHNQLYILDEPTTGLHLDDIKK
                     LLAVLHKLVDAGNTLIVVEHNLDVIKTADWIIDLGPEGGEAGGQIVAEGRPEQVAKVA
                     QSHTGRFLAKVLESSAVRAT"
     gene            16160..>16858
                     /locus_tag="PLS35_09370"
     CDS             16160..>16858
                     /locus_tag="PLS35_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HNI18914.1"
                     /translation="MVDRSAYLARIAYEGPVDPSIETLRRLHRAHVLTVPFENLDIHL
                     GRPISLEPLDLFRKIVTGKRGGYCFELNGLFALLLEDLGFAVTRLAARVLYGAEGVRP
                     RSHELLLVQLGETASVADVGFGGNGLLEPLRLMDGYEERQGADRFRLLAQEQGGYRLQ
                     WEMESVWTDLYSFALDPWLPIDFAFANYYHSHSPDSLFMQRRICTMPTLKGRKTLIDN
                     LLKIRSSNGTQELHV"
BASE COUNT         3408 a         4601 c         5115 g         3634 t
ORIGIN      
        1 tcgtgatggc atacgccttg ctttaatgtc tgccatcaca gtgagcgtga tcggaacagc
       61 gaaaggaatg agccatggta aagatcatca agaaagttgg agatcgggag ttggcctggg
      121 tgatgttggc tgttgcagcc gttgcgctgt ggagcacctg ggggcttgcc agctaagttt
      181 gctgaaaggg tatccgccta cccgtgtgag cgtatctgat ctcagtgaga tgcgcgtgaa
      241 ggtggttccg gtttccagtt gcctgcaagg tcattctctc tctgcacgat cgatcagttc
      301 ttttaacacc accgcctgat tgtgtttcac gtctgaagac ccgaacagca gcgtcagtgt
      361 ctgcgatcga gccagcgcca cgagttcttc gatcgccctg cgttgtgccg gttgtgccag
      421 ctcagcgaga taccgcgagc gaaactcctt ccacttccgt ggatcatgac caaaccatgt
      481 gcgaagcgcg gtgctgggcg ccaattcctt ttgccagtca ttgagccgcg cccgctcctt
      541 cgtgcaaccc ctcggccata tccggtcgac caaaatgcga attccgtcct gtggatccgg
      601 ttcgctgtag atccttttga tatgaaccac ggcgatttta cctcaggtcc aagccgtctc
      661 tagtcctgat gtgagcagca atgccaatgc caaccggaat caccagcgaa ccctcatatt
      721 ccattgtaag tgattcaaaa aaacagttcg actcgagcga ttccgcacga gatgtaccgt
      781 gcgaaatgcg ttcccagttg gcccaccgtg ccgatggcca ttcggtacca tcgcgtagcg
      841 cagcaattac ctgagcttcg tctccgcttt tgacaggacc atatattgca aagccacttc
      901 cttggaccgg aattcaacag cgcatcctcg cagatggtgc gcaagggagg ggcgatgatc
      961 cgtcaatctg gttttccgag tgacgatgtg atcgacaacc aaattctcgt gttgttacac
     1021 cgccaccggc gactgacctt tctcaccttg gcagatgcgc tgccgttgta tacctggcaa
     1081 tccctgtttg cggcgttgaa ccggttgagg acacaagagc aggtcgaact attgcccctc
     1141 gctgcggatt acgaagtgat ttggcggagt ggtcgagaga ccgaacccct gttgtcgaag
     1201 ggggggcatc tgcaggtgcg atgatgcgcg tgtcgtgtcc cgctgttgtc cgatgtctgg
     1261 tgctggtggc agcctgccag atgcctgctt gtgggaacac cgcctcggtg ttcgatttgg
     1321 agacaaacga tccgagacag gatcaggaaa aaatcgctga attctatgca cacgaagcca
     1381 cgaggctgcg gcagatggcg caggattatg accatcgtgt ggccgtgtac gaacggttgt
     1441 ttggccctca ctcggattgg gtcgaaggca cgcgactctt gtcacaatcg tatgaggctg
     1501 cggcacagga acaagaacgg atagccgctc gccatcagcg cctccatgac cggcgttccc
     1561 atgctgctga ccaggaatcc cgctgaatga catgaatgca ggaaggggga gaccatgtgg
     1621 cgatatacga cgatcggact gatcattgca gggctgtgcg ggttgtctgg ttgtggaaac
     1681 acggtcagcc ctggacagcg cggtctccgc tggtacccct tgaccgaggg actgaccacc
     1741 gagaccttga agtccgggtt ctattggaga gcaccctgga acgacgtgta cgtctatgac
     1801 gtgcgtttgc agagttacac ggaactcgtg gacgcgttga gctccgacga tctgttggtc
     1861 aagttgaagg cagccatcat catgcgtccg attgccgaag aagtgtattt cctcgcgcag
     1921 gagatcggcc cggattttta tccgagggtc gttcgaccgg aactgctggc cgcggtgcgt
     1981 agcgtcgtgt cgaactatcc gatggtcgcg gtcccggaaa tgagcgcgga aatcgccagt
     2041 aaagtgcagg cagttgtggt ggagaaactc aagggccgtc acctggaagt gcacagcgtg
     2101 gcgctcgccg atattgagtt agccaagatc gtgctggagg ccgtagagcg caagcaagcg
     2161 aaggagcagg aaaaagaaca aaaggaattc gaactcgtca tcgcggagaa ggaagcggag
     2221 atcgccaggc ggcgtgcgcg cggcgagggt gatgccattc gaatccgatc ggagggcgag
     2281 gcggaagggc tgaagattcg agcgcttggc caggcgaatg cacagcaaac gatcacgaaa
     2341 accctgacac ctgaatatct gcaatacaaa ctctatgaca gccccaacgc gaaaatggtg
     2401 ctgctgccgg atcagttgcg cgtgccgatt ctgatcaatc ccgatcagga tcgcgggacg
     2461 aggtcgactc ctgagagtct catgcgcaca gagcaggagt tgatggggcg gggtcgctag
     2521 atcgcttcgg acggctattg atcgaaggag gccacggatg gcatgcagcc gatgtcaggg
     2581 cttgatggtg gacgagacgc tgttcaatcc aagcgagggc gtgagccata cctgggtgcc
     2641 ggtcgtccgc tgtctcaatt gcggcaatct ggaagatgcg ttgatccggt tggctcgtgg
     2701 catctccgga cgtctggggc gccgcaccag gccgggtccg caacgaaggg gcgtctggag
     2761 tgaggagatc cgcgggcggc aaaaggaatg aatcggctgt ccgtcagtgc gacaggtgga
     2821 gggatgtgaa ctgttgcgat gcaccagagg aaggagacgc catggagggc tatgggaagc
     2881 gcgtgttgat cgcggatgat gaagaaagtg tacgacggct ggttgcgatc gtgctggagc
     2941 aagcagggta tatcgtccat gcggccgtcg atggcgtcga ggggttgggc gagatgaaga
     3001 agcggcgctt cgatgccgtc atttctgatt ttcacatgcc tcgcctggac ggggagcaat
     3061 ttctcctgct ctgtcgcctc atgtggcctg acattccggt ggtattgctc tccgcggagt
     3121 tgctggaact ccccgccgcc ctcaaacagc agggcgcgtg catcctggtt tcgaaaccct
     3181 tttctccgca aatgttattg caagccttgc atgagtcgct ctcattgccc gaaagcgagc
     3241 aggcctcgga ccggatcact tccaaggcgt cgtaatctca atccctttca ttcgatgatg
     3301 cgtagatgtc ccggcgcgct gacaacctgg cgtgcctggt gcatccctag gcggtcatcc
     3361 ttaccaaatg agtcactctg tcattccggc atccagatgg cgcgatagcg cacgggccga
     3421 gcggtgcgaa atctggatac ggtatccgtc ctctgtgatg gaccctccct ccatagagat
     3481 ctgagataga gcaatcacgg tttgtttgac gctgcctggc ctttcacctg caggatcata
     3541 tcttgcttcg ctcagtggtc ccgtgacgcg atgactgacg gtacagagga ggctcatcat
     3601 gaagactctg ctatcacacg catgtgtcat tcttgcatgg agtacactcg ccggatgtgt
     3661 ggccacgtcg gttcgggaac ccttccccgc cttgtccgat gcttctgtga tggnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacgctgg tctcagcgcg ggactggcct
     3841 gcgtaatcct catgcacgac gaatagtcga gggaggcgaa acaatgaatg atggaaccga
     3901 ccggaatgac ggctacgatg tagactccgc ggtgtgggcc tatttgcagc gctgccggat
     3961 atgttcgatg gaggaattgt tccgtcatgt gccgacgttc acagcgaacc aactgtttct
     4021 caccgtggat cggctcagcc gggaaggcaa ggtggtgctg cggtacagga accgtgcgga
     4081 atatgttatg gtgcgaacag ggattgccga cgagtggcct cacgaaatgt cgggcgcacc
     4141 gtttggcgac aggacatgag ggaacaaggt atgaaaaaga agaccgcccc gaaaaaagcc
     4201 gccacacgca cgacaaagca ggttgcaaaa ccagctgtgt ccgagaagca gcctggacct
     4261 gtgcaggata ttcaagtgcg gattgcggcc agggcgcacg agctgtacga acaacgggga
     4321 tgcctggacg gctatcatct ccgcgactgg ttggaagcgg aacgagagat tctggggcat
     4381 ccggtcgaga tggacaaatc ttccgaacgc atttagttct ccttgccggc tggttgtctg
     4441 cagcccttcc aatgtgatcc cggacgcgtc tttgttagac ccggcgctcc tgcctgtcgc
     4501 tcatctcatg gtctcttcca gctgatcgac cattcctgaa atcgcatcga agccggattg
     4561 ccagaacgcg tgggagttca tgtccacacc cacctgagcc aggatcgcct gcggtgcttt
     4621 cgatcctccg gccgccagga ggtccaggta cttggggacg aatgcggcgc cttgttcctg
     4681 gtacatgcga tacagcgcca acacgagcag attcccgaag ctgtaggcat aacagtagaa
     4741 ggggctggca tacagatgcg gaatggtgag ccattcccat tggaactcct gcggtacgcg
     4801 cagcgatttc ccgaactgct gtttgagcag ggtcatatag gtgccggcca gatcattcgt
     4861 tgtggcgccc tgggcgacca tgtcgtgggc tgtccgttcg aaggcgacaa agtacgcctg
     4921 acgcatgacg gtggcgtaga tgtcgtccaa ctgattgacg agcaggctct gtttcaccga
     4981 cttgctcgac tccgaggcca tcaaggcatc tgacagaatg cgctcgccga acacagaggc
     5041 cgtctccgca agcggcagcg tcgagtggaa agtgaacacg ttgtgctgct ccgccatcat
     5101 ggcgtgaacg gcatggccta attcatgggc catcgtcgca atgtcgcgcg cctctccagt
     5161 gaaattcagc atgacatagg gggttacatt cggcgtgacg ctgtagcagt aggcgccgcc
     5221 catttttccc gccttcatcc gagcatcgat gtgccgatcg gcaaagacgc gttgtgcgag
     5281 ttcagccaaa cgcggcgaga acccgtaata ggcgtctagc accatgcgga ccgcgtccgg
     5341 gtaccggtag ctcttttgct cggcgcgatg cggtgcatag atatggtagc ggttcatcgt
     5401 gctgatcttg cagagcttgg ccttgagctt gaagtagcgc tggaagatcg gggcgttttt
     5461 catacagact gctaagagcg cttccaccgc ggagtcggga atgtcgttgc tgaggttccg
     5521 ggaggccagc ggcgtcttaa aatggcggag ttgaaggttt tccgccttcc aatcattgat
     5581 caacgtcttg tagatctctc cgagcagatc ctgctgggat tcgtacacgc gatacatttc
     5641 ccggtaggcg gcttcccgca tcccggcttt cgggcttctc agataggccg tcaacgcctc
     5701 gcggttcatg gtcttgttct tcccgccgat gcgcagcgtg aaggtgaagc cgttggtgac
     5761 cacatcgtag agctgatgca cggcgctttg tccggtgatg ttcttgatgt tgatgatttt
     5821 ctcttccggc tccgagagcg tatgcggttt gaagcggcgg atggtttcca ggtgataccg
     5881 gaagtccccg ctattcgcca tcaagcgctc cgcatttttc tcatccacgc tttgccacca
     5941 cagatcgaaa aacaacaggc gattctggag actggtgagg tgctcttcca ctttggtctt
     6001 gaacgaccgg gcctgcagct gcttggtatc ttcggaaaac cagagatagg agtaggctcc
     6061 cagccggccg gaatcgcgcg cgatctgttc cgacaaggtc aggagattca agaagctttg
     6121 ctcggacatg gttgcggaga ggtccgggcg tgccgcttca aagcgcgtca ccttggctgt
     6181 caggtctttc agatatcgat cgaattgttt cacgggatcg gcgacaaggt cggccaggtc
     6241 ccaccggtcg gcaaattcgc gggatgtcgt ggaacgtcgg gatgatactg ttgcagatgc
     6301 agtcttgcgg gaaacggcca taatcggggc tcctacaaat gagtgatagc cggaatcata
     6361 ccatcctcgc cggctcaggg cgtgtagatt ctttgagggc aggctgttcg ggctgatgca
     6421 ccgttgacag gtcccgcttg aatgttacaa tccggccatg gtggggagcg gggacatgac
     6481 cgaacaagag atcaatcggg ccattcaata tgtgacagcg gcgacgtcct acggccggga
     6541 cacggtggcg gagattctcc ggaccggcct gtccgagttg tctcaactct cgacacaatc
     6601 gacccggcgg ttcgaacggg aagatttgat gggctatctc tgtcagtgga ccatgaaacg
     6661 taccggccat ccggagccgc tggtacgcga agtgctggat ggggccggtc gttggttgga
     6721 tgaggtggcg accaccttag ccgagcagcg tgaagccgag gccaatgccg gcgatgatga
     6781 cgaaaccggc gcggcatcgg cctgaggcat ccgtccgccg tcaactgcgc agtcttcctc
     6841 gcgacgcacg atactgacga atcaccgagc ctggaatcag gcggcgggga gcgagctctt
     6901 caggagttca aggttttttg tcaccatcgc gtctccattc ttggtcgaca cgtcgatccc
     6961 ctggtctgac acctggcggc attcctccac acggttcagt ttcaatagcg attgcgccaa
     7021 ggcatagtac gcagccgagt aggtcggttt caccgtgatg cactgcctca aggccttcgc
     7081 ggcttcctcg aagttggcat cttccatata ggcttttccc agcccgaacc aggcgacatc
     7141 atcgtccggc tccatggcta acactcgttt cagcggttca attcgtggat tcggcatcga
     7201 gtcctccctt tggaggacga taacagcgtt ctcggcgcgt tgtctacgca gtttgacgcg
     7261 tgttaaggtc aaaagacctc ttgtcgtcgg caatgtcgct ggcggtttcc ccggcgcgag
     7321 tactcaatgg gaagaactgg tctcgtttac gatcctcgct atctcgccca cgatatgggc
     7381 gccggacatc cggagtcgcc caaccggctt cgtgcaatca tgcagcgatt agagcaaagc
     7441 gggttggctg ctgaactcac ccgtctagca ccgagaacag ccgaggatga gtggatcacg
     7501 ttggtccatc gccccgagta tgtcgggcat ctcaaccgcg aagcgccgac tagtggccgg
     7561 gtatctctgg atgccgatac ctccatgtcc cccggatcgc tcctcgccgc ctatctggcc
     7621 gcgggcgggg ccttgtccgg cgtggatgcc attatggccg accaggtcca gcatgtcttt
     7681 tgcgcggtcc gtcccccggg ccaccatgcg gaggcagggc gggccatggg gttttgtctc
     7741 ttcaacaatg tcgccatcgc ggcgcgctac gcgcagaaac gttatggggt gcagcgggtc
     7801 ctgatcgtcg attgggatgt ccatcatggc aatggcacgc agcatagttt tgaggcagac
     7861 ccctcggtgt tgttcttcag cactcatcaa tacccgcact acccgggcac cggtcgcgcg
     7921 acggagtgcg gcaccggcgc gggggaaggc ttcaccatca atgtgccgat ggaagcgggt
     7981 gagggcgacg acgactatcg ggcggtgttt cagaaagtgc tggttcctgc cgccgatgcc
     8041 ttcaagcctg agttggttat catctctgcc ggctttgatg cgcatcggga tgatcccctg
     8101 gccagcatgg gcctgacgga agaagggtac gcagacttga cctcgattgt ggccggcatc
     8161 gcgactcggc actgccgggg gagactgctt tcctcgctgg aagggggcta taacctgacg
     8221 gccttggcgg cttcggtcga gcggcacatc cgggcactgg tggcgtcatg aaatggtggc
     8281 tgaagctgtt gctcggcctc gcggtcatct cggggctggt gtacctctat tacacggagg
     8341 tgaagccggt cgtcattttc gggctccggt ccgagtatgc gcatgcgatt cccttccaga
     8401 agattcccga aggcttgacg agcctcaagg ccgaatcctg cggtcaatgt caccgggaga
     8461 tttatgagga gtggaagacg agcattcatg cgcacgccta tgaagatccc ttttttcagg
     8521 cgtactggaa gaaagataaa caggtctggg tctgtttgaa ttgccatacc ccgctggaga
     8581 accaacagcc gaccttggtg aaagatatcc cgcgggggcg cgtggaaaag gcgacccagg
     8641 aaccgaatcc ccatttcgat gccgcgctgc aaaaagagtc catcacctgc gcggcctgcc
     8701 atgtacgcga tggggtcatc ctcggcccct tcgacgattc ggctgcgccg cacccgacca
     8761 aatttgaccc gacgttccgc accgcgcagt tttgttcgcg ttgccacaat gtggtctcag
     8821 gtcctgcgca gttctacaat gtggggccct gcggcacgta tgcggagtat gaaggaaaat
     8881 atttcatgca ggagcggggc tttatctgcc aaagctgcca catgccggag gtggatcgcc
     8941 ccgtggccga gaacggaccc atcaggcggg gccgccggca tctctggcgc ggcggtcatg
     9001 atcccgacat ggtgaagcgg gcggccgcga ttcaagtgaa ggtcgatcct cccaagccca
     9061 agccgggcga ccaggtcacg atagccctga ccctcacgaa tgccggagcg ggacataagc
     9121 ttcccaccgg cgatccggac cggcacttca ccgtcgaatt tacggtgaag gacagcaatc
     9181 agcagattct ggccgaaaag agccatacca tgggtcgctg gattatgtgg cagccggcca
     9241 ttgtggagtt gtacgacaac cgcttgctgc cgctggcgag ccgggaatat caattcgcct
     9301 accggatgcc ggaagcatcg aagggggtga ccttgaaggc tcgcgttcgg taccacattc
     9361 tgaccgacgg tcagcatgag atgttgaaga agaaatacgg tctcatggcg gacgatccct
     9421 acgccttcac ggtgtatgaa cgagaggtgc cgctgaacga acagctcgcg gccgcgttcg
     9481 ctgatccctt gcctgagccg ccaccgatgg cctgcgcgaa tccatcagct ccacaaggct
     9541 aaggcgtcat cgaacgggat cggacaacct ccaccacatc cgttcgcgac cattaaacgc
     9601 actcgatatc ctgcaagggc tgaccgttga tcagcctccg ctcatcattg taaaggccaa
     9661 agcactggca cgtgcatttt ccttgctcgg ggaggccaga ttaattggac gaaacaaacc
     9721 ctttgagaat acgcgcgatg cgcggcgcat agggtatttt ctgtcaaatc ccgatggcgc
     9781 aaagctcgat aagcgtatca atctgggcga gcaggaggca ggtaggaggg atcttgcggg
     9841 aacggtgaac cgcaggagag ctgtttcttt ccgtgcgttc cggctctgtt ttatctgcgt
     9901 ttgcctggta gtgcggcgaa gacggtacat gcttgcggaa ctgctcatcg gacatactga
     9961 ttcgaaagtc gcacagccga cagacatacg aaccagtcag gtacacgtcg cccacgcact
    10021 cgagttggta ctcatggtca cattctggcg tcatcaggtt tttgtagcat atcgatcatg
    10081 ctaagacacc tgggtggata ccttggaagg aaatgcctat gcaccacatc agtgtaggaa
    10141 ggctcaacga ccgacctgtg agttttcgta tcgacgccaa tgaaatccct tccgcgtttc
    10201 acaatagtcc tgttgtgttc attggtgagg gttcggtaag ctgttggccg ggttaatcac
    10261 tcaccgagga tacgcgatgc aacatccatt actcatcgat tgcgagacac ttcagacacg
    10321 gttagggcag ccaggcctgg tgatcctcga tgtgcggggc cgatccacgt acgagttcgg
    10381 cgggcacatc cccggcgccg tgcattccac ctggcacgaa tacagtgatc ccgaggcgat
    10441 cgccaaaggc ctcttggatc cggatgtgaa acggatcgag aagcgggtgc aagcactcgg
    10501 aattaatcgg gacaatgagg tcgtcatcta ttccaacccc ttcgataact ggggtgatga
    10561 aggtcgcatg ttttggatgt tggaatacct cggccacaaa aacatcaaag tcctggatgg
    10621 cggttgggtc aagtggattc acgagcgccg gccgttcgag catggcgcgg tccgtcccac
    10681 gccaggcaac ttcaccgtct cgccggtggc ctccgcgatc atcatgaagg atgaattgaa
    10741 gggtatcgta cgcaagcccc atccgaccac cacgattgtg gacgcgcgga gtctggaaga
    10801 gtttctcggg aaggaagtgt ccggcattcc gagacccggc catatccccg gcgcggtgca
    10861 tgtggcatgg agcggattcc tcaatgcgga tgccacggtg aaggatcttg acgccattca
    10921 tgcccagttg gaactcaaag ggttgaatcc ggcccatgaa acggtgtgtt actgcacggg
    10981 cggggtccgg tcgggatggc tctattttgt gctgcgcctc gcgggctttg agcggctgcg
    11041 taattatccg ggctcctggt gggagtggag ccgggatttc gcctgcccgg tggagaagga
    11101 tctgcttgcc ttgcaaaaac tgctcggctt gtcggagtcg caggtgccga ccggcatgcg
    11161 tccgtcttga caggataggg gcccgtcttt atgctgaatt agaatgtatt cggattgatg
    11221 cagtgcacat acttacagag gaggcgtttc atgcagactg ctatgtggcg gggagtcgtg
    11281 gtggccggag tcatgatggc gctcgtcgga gcgccgatgg tatccagcgt ggctttcgcg
    11341 gcggggaata agcatcaagc ggaagccgtg gagcatgcca aggaagcggt ggcgcatggg
    11401 aagcagggcc atgcggatgc gctggtgaaa catgccgagg cggcgctcaa gcatgccgaa
    11461 ggcgccatgg cggagaccaa gaatcctcat gtgggtgaag ccatcaaggg cttgaaggac
    11521 gggatcgaac acggtaaggc aggccatgcg gatgtggcga ccaaagcggt cgaaaacgcg
    11581 cttccccatc tgtccgaagg tatgtaagcg gttcttgcgc agcgacgacg cgtacgataa
    11641 aacgggcagg ggcatccctg cccgtttttc tttgtgtaga aaggcagcat gatgcgggtt
    11701 ggatatctgc aaggtgatcc ggtgttcggc gaggtcgcgc acaacctcga tactgtgaca
    11761 gctcagctcg agcacgcaga ggctgatctg ctggtgctgc cggaattgtt tgcgaccggc
    11821 tatcagttca cgtcacagga agaagtgtct cggcttgcgg aagcggtgcc ggatgggccg
    11881 acaacccaac gactcctgga gatcgccgcg cgccgcaaga ccaccatcgt cgccggattg
    11941 gcagaacggg cggggacgaa atattttaat tccgccgtcg tggtggggcc aaaggggttc
    12001 atcggctgtt atcgcaagac gcatctgttt ttcgaggaaa cgctgttttt caatcccggc
    12061 gatagtggct ttcaggtctg ggatatcggt gcagccaaaa tcggtgtgat gatctgcttc
    12121 gactggtact atccggagtc ggctcgcacc ctggcactgc tggctgccga gatcattgcc
    12181 catccctcca acctggttct gccgaactgt ccggattcca tggtgacacg gtgtctggaa
    12241 aaccgcgtgt tcagcgtcac ggccaatcgg atcggcagcg aagcacgcgg cggaaaagac
    12301 cggctgacgt ttatcggtct gagcgaaatc gtcagccccc gcggtcaaat cctgcaccgt
    12361 gcgccccgcg atcgtgaaga gctgaccatc atcgagatcg atcctgcgga agcgaggcga
    12421 aaggcgctca atagctataa tgatctcctg cgggaccgcc gcccggccat gtatggggtc
    12481 tgagcgaagg tcgcttgaat tttctgtctc gacttgcgcc ccttcatgcc tcacggtagg
    12541 atgaaccatg acaactccac tcactaaagg cgtgctgctc gtggcgacgc cggcattgaa
    12601 tgatcccaat ttcaggcagg cggtcgtgct gctctgtgaa cacgggcctg aaggagcgct
    12661 cggcgtgatc gtcaatcgtc cgacggccat gtccatctct gaagcgttgc cacaggttcc
    12721 catcctggaa ggccaaccac acgtgctcta ctcgggcggc cctgtgcaaa ccaatcaagt
    12781 gatgatgctc tatcgcatca gcgacacgcc ggagaattcc catcaggtat tcgatggcgt
    12841 ctgcctcggc ggcgacctgg aaatcatgga acggattctc atggagcagc ccggaaagga
    12901 atcattccgg gcctacttag gatactccgg ctggggacct gggcagttgg agtcggaaat
    12961 gcagaccggt tcgtggatta cgctgccggc tgatccttcg attgtgtttg agaaggaagc
    13021 ggcgcgcatt tggtcggata tcgtgttgtc gcttgatgag acctctcgcc actacgcgga
    13081 tatgcccttc gatccctcct ccaattagcc gcgccgagcg gcgttcatca cgactgccgg
    13141 cgttcctgat accacctcgc tgactgttac cctctcccac tcattgcagg catgcctcgc
    13201 ctctcaggtt gcgcgcacag cggaactttc cagtaccttc gccagaaacc gtcccgtatg
    13261 cgactgcgcc accttggcga cctgttccgg tcgtccttcg gcgacaatct ggcctccggc
    13321 ctcgccgcct tccggaccga gatcgatgat ccagtcggcc gttttgatga catcgagatt
    13381 gtgctcaacg acgatgaggg tattgccggc atccacaagt ttgtggagga ccgccagcaa
    13441 cttcttgatg tcgtcgaggt gcaggccggt cgtcggttca tccagaatat agagctggtt
    13501 gtgtgccgac ggatctttga gttccgcggc gatctttaac cgctgggcct ctccaccgga
    13561 cagagtgttg gccgcctggc cgagccgcag ataccctaat ccgatcgatg agagcagata
    13621 aagcttttct gtcagcttcg gcgacccgga aaaaaacgcc tgcgcttcag tcaccgtcag
    13681 atttaacacg tcgtgaatgg ttttcccgcg atacttgata gcgagaatct ccggtttgaa
    13741 tcgccggcca ttgcactgtt cgcacgttgc atagatgtct tcgaagaaat acatctccag
    13801 cttttcatac ccgtttcctt cgcaacgttc gcaccggccg ccggccgcat taaacgagaa
    13861 atgtcccggc gtgaggcctt gtcgcagcgc gtcccgttcg gaggcaaaga gctggcgaat
    13921 ctcgtcgaag gccttgaggt aggtaatcgg gttcgagcgt ggagtacgac cgatcggttg
    13981 ctggtcgatg aggcggacgc ctttgagata ttcgagtccc ttgatggctt gaaacctccc
    14041 catcgggagt gattcgatcc gaaaggcccg cgcggcggcc cggtagaccg tgtcctcgat
    14101 gagtgtgctt tttcccgagc cggacacgcc ggtgatgcag accagcatat gcaggggaat
    14161 gcgtactagt agatttttga gattgtgttc ggcggctccg gcaatgctca gcaccttgcc
    14221 gttcccggag cggcgggttt tcggaagcgg gattcgttcc tcacctcgca ggtaacgggc
    14281 agtcagggat ttcgggtcgc ggatgaatgg tgcacgcggc gccgcgcaga ccacttggcc
    14341 gccttgttct cccgaagccg ggcccatttc aacgatatgg tcggcagcct gaatcatcag
    14401 cgggtcgtgc tcgacgacaa cgacggtgtt cccttccgtc gccaggtcgc ggaggatgcc
    14461 ggccagggtg tctgtgtctc gcgcatgcag tccgatggtc ggttcgtcca gtacgtatag
    14521 ggttcccacc aaccgggagc ccagctggtt ggccagggct atccgttggg cttcccctcc
    14581 ggacagcgtt tttgtttgtc gggagagcgt aaggtagttc aaccccactc gcagcagaaa
    14641 attcagcttg gcctgcaatt gccggaggat atccttggcg atttccgcat cgaatgccgg
    14701 taatgccaac cgctgaaacc attccgtggc agcctgaatg gtgaaatcgt tcaattgaat
    14761 gaaatcgtgc ccggccaatt tgacgaagcg cgcggccggc ttcagccggc tgccctgaca
    14821 gatcgggcag gttgccgggc tgcggtagcg actgagcagg actcgcacgt gcagcttgta
    14881 gcgcttcgtt tccagatagt cgaagtattg ccggacgcct tccacctggt cgctgccttc
    14941 ccaaatcagc tgctgcacct cgctcggcag ttcctgaaac ggagccgtca aatcgacctc
    15001 cagtttcttc atggcgagca aaagctgttt ctgccaccag tccgatcccg gtttgctcca
    15061 gggttcgatc acgccgccgg cgagcgactt actgcgatcg ggaatgacca ggtcccggtc
    15121 gtagcgcagg atattgccga agcccttgca ctcagggcag gcgcctaatg gatggttgaa
    15181 tgaaaagagc agggggcgca acggctcgaa ggtccggccg caaccctgac accgaaagtg
    15241 ggtactatag gtccggagcc catggccgat gacatccacc tggcagaccc cgtcagcctc
    15301 ctgaaaggcg acttcgatgg cttcgatcaa gcgatggcga ttgtcaggtc tcagtacgag
    15361 gcgatcgagc acgacctgaa ggccggactc gcggtgttcc ggcagtgtga cgttatcgtg
    15421 gaggtccagg atctcgtcgc cgcagcgcag tctggtgaac cctcgcttcg tcaacgaatc
    15481 cagcagcgac cggtcgtggc ctggtcccag atcgttgacg gggaataaaa tcatcgcccg
    15541 cgcatcggga aattgcgcca gcagttcctc ggcaagcgaa ccggggtggt acccgcgagc
    15601 ttcctgatga cagtccgggc aaaccggttt gccgattttg gcgaagagca agcgcagcag
    15661 atcggcaact tcggtcgccg tgccgacggt cgagcgcgcc gtgcgaatcg gattcttttg
    15721 ttcgatggca atggcggggc gaatgttggt gatgcggtcg acgtcggggc gattcacttt
    15781 gtcgagaaac atgcgcgcat aggtcgagag ggattcgaca tagcgccact gtccttccgc
    15841 aaacaacgta tcgaacgcca gggaggattt gccggaccct gacaggccgg taatcgccgt
    15901 aaccttgttg tgagggatgc gcaatgaaat gtttttgaga ttgttctgac gtgccccttc
    15961 gatgatcagt tcgtcggttg atcgagtcgg gggtgtacgg gatgccacgg cgaagcgctc
    16021 cttctcatgg atgaccgacc atcgtagcaa gcgctgccgt cgtctgcaat gtcatcgacc
    16081 tgccagcgat gagactcgtg cgatcggcct ggatggactc gtataatggc gccatcatat
    16141 ggacgactga gaggatggta tggtggatcg ttctgcttat cttgcgcgca tcgcatacga
    16201 agggccggtg gatccatcca tcgagactct ccgtaggttg catcgtgctc acgtgctgac
    16261 cgtgccgttc gaaaatctcg atattcacct tgggcggccg atctcgctgg agccgttgga
    16321 tctctttcgg aaaatcgtta ctggtaaacg gggcggctac tgcttcgaac tcaatggcct
    16381 gtttgcgctg ctattggaag accttggatt tgcggtgacc aggctggctg cccgggtgct
    16441 ctacggtgca gaaggggtaa ggccgcgcag tcatgagctg ttgttggtgc agcttgggga
    16501 gactgcatcg gtcgcggacg ttggctttgg aggtaatgga ttacttgaac ctttgaggtt
    16561 gatggatggg tatgaggagc ggcagggcgc cgatcgattc cgcctcctcg cgcaggagca
    16621 aggcggctat cggctgcaat gggagatgga gagtgtctgg acagatctct attccttcgc
    16681 cctggatccc tggctgccca tcgactttgc ctttgccaac tattaccact cccattcgcc
    16741 ggactccctc ttcatgcagc gccggatttg cacgatgcca accctcaagg ggaggaagac
    16801 gctaatcgac aacctgctca agattcgaag ctcgaatggg acgcaagaac ttcatgtg
//
LOCUS       DAOQEW010000070        16855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_901_length_16855_cov_8.380298, whole genome shotgun sequence.
ACCESSION   DAOQEW010000070 DAOQEW010000000
VERSION     DAOQEW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_16855_cov_8.380298"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1015)
                     /locus_tag="PLS35_09375"
     CDS             complement(11..1015)
                     /locus_tag="PLS35_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNI18915.1"
                     /translation="MSNGPTTLTLHSMADVWDVYREELEGVEEQIRKNLDSSVDLVNT
                     VAAHILNSGGKRVRPLFVLLSAHLCGYAGQDHQALGSLVEFIHTATLLHDDVVDDADL
                     RRGRRTASKVWGNQISILVGDYLYSRAICQIVDFRNQGINEALSEACRKMAEGEVLQL
                     YYNGNPLMPEPEYLRIIEHKTAGLIAASCKIGAIVGGATEELQEALFRFGQRLGIAFQ
                     LADDTLDYTANGEHLGKTLGQDLRQGKATLPLLHLLQHCPEPDRQLIKDRMETRTLTD
                     EELRRIVALMQEYGSIAYAMERAHAFVAAAKRDLDLFHDNTAKRALSIAADYMVTRDR
                     "
     gene            1251..5771
                     /gene="gltB"
                     /locus_tag="PLS35_09380"
     CDS             1251..5771
                     /gene="gltB"
                     /locus_tag="PLS35_09380"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNI18916.1"
                     /translation="MNVPGLPPKQGLYDPQHEKDSCGVGFVVDIKGQRSHQIVQQGLQ
                     VLESLTHRGAQGCDPCTGDGAGILLQVPHEFFKRAAKDSGIKLPGAGEYGVGMVFLPP
                     DADARAQCETVFTRVIKDAGAKLLGWRDVPVKSDAIGPVARSTEPFMRQVFIARGIFT
                     DEEFERRLYVIRKCAERAVRESAIEGRDYFYIPSLSSSTIVFKGLLLPHQIPQYYQDL
                     TDSSLTSAMALVHSRFSTNTFPTWPLAHPYRYICHNGEINTLKGNVNWMRARQGRLNS
                     ELFGEDMKKLFPIVYENQSDSASLDNALEFLVLGGRSLPHAMMMLIPEPWVANPQMDL
                     DRRGFYEYHAAMQEPWDGPAAVCFTDGKLIGATLDRNGLRPCRYQVTTDGLVVLASEA
                     GVLPMDPQRIRQKGRLMPGRMFLVDTEQGRIIDDEEVKADIVRRKPYRSWVTEHRISL
                     DELPDPINVPQPDHPTIRQRQQAFGYTIEELKMVITPMVVEGQEAISSMGTDTPLAVL
                     SERPQLLFKYFKQLFAQVTNPPIDPIREELVMSLTTSIGPKPNLMDEHPESARRIRVK
                     QPILTNADLHKIREIADPHFKSKTLKMLFRVAEGPEGLGAAVDDLCRQASQAIREGYK
                     FLILSDRGVNAEWAPIPSLLGVAAVHHHLVRECTRTEVGLTVETGEPRDVHHFACLIG
                     FGAGTVNPYLVFESLVDLERDGYFPEGLDAATAEGKFIKAINKGLLKIFSKMGISTVQ
                     SYCGAQIFEAIGLNHELIDRYFTGTPSRIEGIGIREIGEETLRRHRVAYEPVPIRQLD
                     FGGEIHYRIQGEHHNWNPDTIYKLQHATRNNDPKTFAEFSQLVNDESKRRSNLRGLLE
                     FKFLPEPIALNEVEPAKDIVKRFTTGAMSFGSISKEAHETLAIAMNRLGAKSNTGEGG
                     EDPERFEPLPNGDSRNSYIKQVASARFGVTSHYLVNAKELQIKMAQGAKPGEGGQLPG
                     HKVDENIARLRYSTPGVQLISPPPHHDIYSIEDLAQLIFDLKNANADAAVSVKLVSEV
                     GVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGVPWELGLAETHQTLVLNDLR
                     GRIRVETDGQMKTGRDVAIAALLGAEEYGFATAPLIIEGCIMMRKCHLNTCPVGIATQ
                     DPVLRKKFTGQPEHVVNFFFFIAEELRQIMAKLGFRTINEMVGRVDKLKIHKAVEHWK
                     AKGLDLAPLLKMPEVGPEVPRYCVQKQDHGIAEILDRALIEQCAPAIDRGQKVTLELP
                     IRNLNRTVGTMLSSQVSKKYGQDGLPVDTITIKFNGSAGQSFGAFLSRGITLILEGES
                     NDYIGKGLSGGKIIVFPPKNAIYTPEETILVGNTSLYGGTQGEAYFYGMAGERFAVRN
                     SGVRAVVEGTGDHGCEYMTGGVVAVLGRTGRNFAAGMSGGVAFVLNELDKFQSRCNLG
                     MVELEPVVSDEDKRLLHDMITSHFLYTGSRNAKRILDGWEAILPKFVKVMPIDYKRVL
                     AERKAAAAKVQK"
     gene            5851..7284
                     /locus_tag="PLS35_09385"
     CDS             5851..7284
                     /locus_tag="PLS35_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNI18917.1"
                     /translation="MGDPKGFLKYAREGPKRKPIELRVLDWKEMYEPIPEEKLKVQGA
                     RCMDCGVPFCQGNTGCPVVNLIPEWNDLVYRGRWKDALKALHTTNNFPEFTGRLCPAP
                     CEGACVLGINSDPVSIRVLEWNIIDRGFNEGLVEPALPVRKTGKTVAIIGSGPAGLAA
                     AQQLARAGHSVTVFEKADRIGGLLRYGIPDFKMEKWVIDRRLEQMKAEGVEFKTGVTV
                     GKDVTGEQLRQEFDAVGLTMGAEMARDLPVPGRELKGIHFAMEYLTQQNKRTAGISVS
                     EEPITAKGKRVVIIGGGDTGSDCLGTAHRQGCSEAHQFEVLPEPPPSRSSSTPWPLWP
                     MQLRTSHAHEEGCDRQWSVSTTKFTGHNGHVTKLHANRVKFEGGKFVAIPNTDFELDA
                     DLVLLAMGFTGPVRNGFLDSLGVNYDARGCVTVNENFMTNLDGVFAGGDTKRGASLIV
                     WAIAEGRKMAAGINQYLQANKSAKSGR"
     gene            8474..8911
                     /locus_tag="PLS35_09390"
     CDS             8474..8911
                     /locus_tag="PLS35_09390"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNI18918.1"
                     /translation="MTQQTYPRSPKVLLGGIAHLGRFIDKVRLRHAGKIQDYNYITVG
                     FDKYLIDFLQIDAKAFEQQVLAGESDEKLLSWVKTHGRKHSEEEIAQWSKGLLAGGPK
                     DDAAKQRFQGRVQDIATKRGVPLTSLPPVTTWADVIELDEERM"
     gene            complement(8940..9581)
                     /locus_tag="PLS35_09395"
     CDS             complement(8940..9581)
                     /locus_tag="PLS35_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18919.1"
                     /translation="MHRSIALLLFVITTSTASLLVTAEPSHAQGGGSQVTITPTDSKV
                     IAWSAAGGGVEESLQPNETVISSAARGHTGFVQTSSRLLGFSAGLRQWREVSIGVEER
                     IGRHQLLPFLILAHSNQQVFGFQETRGHWTTIALGPNETVSQLRGQGHVAVAITNERA
                     LGFSTYTGGFFNIDWTPNERVLSVDGSQNGMVVRTSSRTVIFKSQSTGWTEVR"
     gene            complement(9743..10234)
                     /locus_tag="PLS35_09400"
     CDS             complement(9743..10234)
                     /locus_tag="PLS35_09400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI18920.1"
                     /translation="MTRVSLAGLLILCAIVLLSRPLHAQTGSPTIEDGSKVTLQMTIT
                     LPNDHVMIPPQTSEYTHGEKQLLPGLEEALVGLHSGDTKRVELDADHAFGQYDAGKKM
                     TVPRAQLPHTIARGDMATTAEGRPFTVVSLSDESAVVDFNHPLAGKHIVLDIVVLNVK
                     PKA"
     gene            complement(10290..10571)
                     /locus_tag="PLS35_09405"
     CDS             complement(10290..10571)
                     /locus_tag="PLS35_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18921.1"
                     /translation="MKSIIVIGSLVCVLAMPIVAPLESQASLTYTRVKVESVDQASQS
                     LTFKTAEGEVWTLAALSADMLSGLHKGDTCSLEIDFDNRVTKIVKIDSP"
     gene            10783..12018
                     /locus_tag="PLS35_09410"
     CDS             10783..12018
                     /locus_tag="PLS35_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18922.1"
                     /translation="MSQDDASFPGSESGGITVALALSAIVTLPLMGPWLSGQSLRPYL
                     EFPPLSTGVVTQVAFSWPVFVGLSALIVCLLAPFIVRVARTNSRGVSTVAGQERTPYG
                     SVRRQCVLPHAFPWWGWGAVAWTICAWFLAWTRFEWMQPWQPHTFTPLWLGYIVVVNA
                     LTYRSTGRCMLIHRPRYLLSLFPLSACFWWLFEYLNRFVQNWYYVGGGTLTTGEYLVR
                     ATLPFATVLPAVLSTAEWLTACPRLSAGLDHVVTINFSRRVWGWLLLSGAAAGLLGIG
                     LWPDYLFPLVWVAPLLVITALQLIRNEPTIFSETAHGDWRTLWAVALAALVCGWFWEM
                     WNFYSLAHWQYAVPFVQGVTLFHMPLLGYAGYLPFGLECWAVADLCLARKFAGGVAYY
                     RAAEPETDFAQRNPTVAER"
     gene            complement(11955..12899)
                     /locus_tag="PLS35_09415"
     CDS             complement(11955..12899)
                     /locus_tag="PLS35_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI18923.1"
                     /translation="MIPRTLFPRIASLLHALSLVILSVAATWLNPALSIAARAPTPPP
                     TAELAKHLTSIAELAASSPAVLIPEGPFLLGSIRVDDDPYGMGTQFDDTELPQHRVWL
                     DAYEIDRDEVSLGEYLTYLHQRKIHPSKDLQHLIWHVITVHSVSDETLAQWPALYVTW
                     KEADNLCRASGKRLPTEAEWEKAARGPDGNRFPWGESLPDNSLAMFGQHHVHEIPILA
                     PVSSGEPGKSYYGVHHMAGNVAEWVNDWFGFDYYAYMPERNPPGPTSGRYKSLRGGSW
                     KSRPIMLRTATRSGAPADQRSATVGFRCAKSVSGSAAR"
     gene            complement(12899..13654)
                     /locus_tag="PLS35_09420"
     CDS             complement(12899..13654)
                     /locus_tag="PLS35_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18924.1"
                     /translation="MIKASQRSLAPLFSLAVLSWCVGCTPDESPFRQENENLRKQLAK
                     QESLLNSLQDGNKVMQQQIDLLNQELRDAKKQTEHAQAEAKSLQTEAKTLGTKLDAQL
                     AESKKLSGEIQRTAAKAAQATDSIRVEEKGSQVEDLPRPLPAVAKAAEEALTRNGYRI
                     RVTVKTDQKAVFVTERKISIPTSVETAGSRNQYVVSLQALPSNVTRLSVKAEFERVAQ
                     GGRVLAVDAEEIAEIERRLIAEIGKAVASPSKL"
     gene            13794..14423
                     /locus_tag="PLS35_09425"
     CDS             13794..14423
                     /locus_tag="PLS35_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18925.1"
                     /translation="MSLALFQPAAAAVSIAAMAEPAEPVPAEDYAFFDLAVEDKFLTS
                     HTKLIVIERMTATHLQPDEPQLPTLAWFAEREWFDGRLPQDLVRDFVGKNQRPSRLDT
                     RFSFGVRYRFMMGEGGGDAEASVPLIPATWLVQEEGGAPDVIDHLAFSRVGLNLRADQ
                     ALLYVANPRPDGTGAGFLLWFVRQQKVWSLHDTEVLWVSRPDQGQSGRR"
     gene            14471..15100
                     /locus_tag="PLS35_09430"
     CDS             14471..15100
                     /locus_tag="PLS35_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI18926.1"
                     /translation="MIRKTFSVPPLGCNCSIIGDPVTKQAIVVDPGGAPERILQEVRA
                     MGLTVVSIVHTHAHFDHFLASGAMKQATGAALCLHPDDLPLWDILEMQCRMFGVPYVP
                     VPQPDYWLKDEERVAVGEMEGVALHTPGHTPGSMCFHFPAAKLLVAGDTLFRGGIGRT
                     DLWGGDFDAIEQSIRERLYTLDADTAVVTGHGAETEIGLERETNSFVRA"
     gene            15218..15733
                     /locus_tag="PLS35_09435"
     CDS             15218..15733
                     /locus_tag="PLS35_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNI18927.1"
                     /translation="MIATARRWLVCPLMSFLLGFSAADVLVYADEPGSSPQVTISQVR
                     VGLSDHGPVVLLSADGKTIPVFVDHTVAASIQAALTGEKLPRPLSHELMHTILESLGG
                     RVVRTVITLKAGTYYGSLTVAFQGQEKVFDSRSSDSIALAIHFHAPILVARDMLDAVG
                     KSSLEPKPESL"
     gene            15874..16197
                     /locus_tag="PLS35_09440"
     CDS             15874..16197
                     /locus_tag="PLS35_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI18928.1"
                     /translation="MKNQRKHERVHVQFRSHFSMKGKMLAGDGDLTDLSPGGCRIISP
                     VEVLVGSEVELCIFPGDNANPILIDAATVRWCRPNEFGLSITKIRGPVQRQLTDVWRK
                     LAKPA"
     gene            complement(16448..>16855)
                     /locus_tag="PLS35_09445"
     CDS             complement(16448..>16855)
                     /locus_tag="PLS35_09445"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNI18929.1"
                     /translation="GRKMSKSYGNAINLSDTEAVVRQKLKTMITDPARVRRTDPGNPD
                     VCPVYDFHKIFSPLPVIEQVNQDCRKAAIGCIDCKKLVADRVVERLAPMWEARANLTQ
                     HPGRLDDIAEDGRRRATAVSSQTMAEVREAMKI"
BASE COUNT         3296 a         4693 c         5207 g         3659 t
ORIGIN      
        1 cggcggcaac tcagcggtcg cgagtcacca tataatcggc ggcgatggac agggcgcgct
       61 tggcggtgtt gtcgtggaac agatcgagat cgcgtttcgc ggcggcgaca aacgcgtgcg
      121 cccgttccat ggcgtaggcg atggacccat actcctgcat caaggcgacg atgcggcgca
      181 gttcctcatc ggtcaaagtc cgggtttcca tgcgatcttt gatcaactgc cggtctggtt
      241 ccgggcagtg ttgcaacaga tgcaacagcg gcagcgtcgc tttgccttgc cgaaggtcct
      301 gccccaatgt tttgcccaga tgttcgccgt tggcagtgta gtccagcgtg tcatccgcca
      361 gttggaatgc aatgcccagc cgctgtccga agcggaacag cgcctcctgt aattcttccg
      421 tggccccgcc gacaatcgcg ccgatcttgc acgaggcagc aatcaatccg gcggtcttat
      481 gctcaatgat gcggaggtat tccggttccg gcatcagcgg attgccgttg taatacagtt
      541 ggagcacttc gccttcggcc atcttccggc aggcttccga gagtgcttcg ttgatgcctt
      601 ggttacggaa gtcgacgatc tggcagatgg cccgtgaata gaggtaatcc cccaccagaa
      661 tactgatttg gttgccccag accttgctcg cggtacggcg gccccggcgc aggtcggcgt
      721 cgtccacgac gtcgtcgtgc agcagggtgg cggtatgaat gaattcgaca aggcttccca
      781 gggcctgatg gtcctggccg gcgtatccgc agagatgggc gctgaggagc acgaagagcg
      841 gtcggacgcg tttgccgccg ctgttcaaga tgtgggcggc gactgtattg acgagatcga
      901 cgctggagtc gagatttttc cggatctgtt cctcgacccc ctcgagctct tcacggtaca
      961 cgtcccagac atcggccatg ctgtggagcg tgagggtggt cggtccattc gacatggggc
     1021 ggatagtagg gcaggaaact ccactaagtc aagcactggt cggagggttt gtacttcagt
     1081 ttgcgtcgcc gttctcttga cagtttcacg gcccatccag taaggtgacc tctcgctgat
     1141 ttccccaact gaatggtagc agtcaccgtg gtcgtgcgac agcttcaacg tccccgagag
     1201 gggcgtctgt tccccggttt attgcctgcc tctagacgag aatacgtgcc atgaatgtac
     1261 cagggcttcc tcccaaacaa gggctatatg atccgcagca cgagaaagac tcgtgcggag
     1321 tcgggtttgt cgtcgatatc aagggccagc gctcgcacca gatcgtgcag caggggcttc
     1381 aggttttaga aagcctgacc catcgcggtg cgcaggggtg tgatccctgt accggagacg
     1441 gcgcaggcat cctgcttcaa gtcccgcatg agttcttcaa gcgtgccgcc aaagacagcg
     1501 gcatcaagct gcccggagcg ggagagtatg gcgtcggaat ggtgttctta ccgcccgatg
     1561 ccgatgcccg cgcgcagtgc gagacggtgt ttacccgggt cattaaagat gccggtgcga
     1621 agctcctcgg ctggcgtgat gtgccggtca agagcgacgc cattggaccg gtggcgcgca
     1681 gcactgagcc gttcatgcga caggtcttca ttgcccgtgg catcttcacg gatgaggaat
     1741 tcgaacggcg cctgtatgtc atccgcaagt gcgcggaacg ggccgtgcgc gaatccgcca
     1801 ttgaaggccg ggactatttc tacatcccga gtctctccag cagcaccatc gtgttcaagg
     1861 ggctcctgct cccgcatcag atcccgcagt attaccagga tctgaccgac agcagcctga
     1921 cgagcgccat ggcgctggtg cattctcgct tcagcacgaa tacgtttccc acctggccgc
     1981 tggcccatcc ttatcgctac atctgccaca acggtgagat caatacgttg aagggcaatg
     2041 tgaattggat gcgcgcgcgg caggggcggc tgaactccga gttgttcggc gaagacatga
     2101 agaagctctt cccgatcgtc tatgagaatc agagcgactc ggccagcttg gacaatgcgc
     2161 tggaattcct ggtcttgggc gggcggtcat tgccgcacgc catgatgatg ctgatccccg
     2221 agccctgggt ggccaatccg cagatggatt tggatcgccg tggattctat gagtaccacg
     2281 ctgctatgca ggaaccctgg gatggtcccg ccgccgtgtg tttcaccgac gggaagttga
     2341 tcggggccac gctggaccgg aacgggcttc gcccctgccg gtatcaggtg acgaccgatg
     2401 gattggttgt cctggcttcc gaagccggtg tcctgccgat ggatccgcag cgcattcgtc
     2461 aaaaaggccg gttgatgccg ggccgtatgt tcctggtcga taccgaacag ggccgtatca
     2521 tcgacgacga ggaagtcaaa gccgatatcg ttcgtcgtaa gccgtaccgg tcctgggtca
     2581 ccgagcatcg catttccctg gatgagttgc ccgatccgat caacgtcccg cagcccgatc
     2641 atcccacgat ccggcagcgt cagcaggcgt tcggctatac catcgaagaa ctgaagatgg
     2701 tcatcacccc catggtggtg gaagggcagg aagccatctc ctcgatgggc accgatacgc
     2761 cgcttgcggt gttgtctgaa cggccgcagc tgctctttaa atacttcaag caactctttg
     2821 cgcaggtcac gaatccgccg atcgatccga tccgcgaaga actcgtgatg tccctcacca
     2881 ccagcatcgg ccccaagccg aatctgatgg atgagcatcc ggaatcggcc cgccgcatcc
     2941 gggtgaagca gccgattttg accaatgccg atctgcacaa gatccgggag atcgccgatc
     3001 cgcacttcaa gagcaagacg ttgaagatgt tgttccgcgt cgccgaaggt ccggaaggat
     3061 tgggcgcggc ggtggacgat ctctgtcggc aggcgtcgca ggcgattcgt gaggggtaca
     3121 agttcctgat cctcagcgac cgcggcgtga atgccgaatg ggcgccgatc ccgagtcttc
     3181 tcggtgttgc ggcggtccat caccatctgg tgcgggaatg cacgagaacg gaagtgggcc
     3241 tgacggtgga aaccggagag ccgcgcgacg tgcatcattt cgcctgcttg atcggcttcg
     3301 gagccggaac cgtgaatccc tatctggtct tcgaatcgct ggtggatctg gaacgcgacg
     3361 gatactttcc ggaagggctg gacgcggcga ccgccgaagg caagttcatc aaagccatta
     3421 acaaaggctt gctcaagatt ttctcgaaga tgggtatctc cacggtgcag tcctactgcg
     3481 gcgcgcagat cttcgaggcg atcggcctca atcatgaact gatcgaccgc tacttcaccg
     3541 gcacgccgtc gcggatcgag ggtatcggca ttcgggagat cggggaagaa acgttacgcc
     3601 gccatcgcgt ggcctacgaa ccggtgccga tccgacagtt agatttcggt ggcgagattc
     3661 actatcggat ccagggcgaa caccacaact ggaatccgga taccatctac aagttacagc
     3721 acgcgacgag aaacaacgat cccaagacgt tcgccgagtt ttcgcagctg gtcaatgacg
     3781 agagcaaacg ccgctcgaac ctgcgcggcc tgctggagtt caagttcctg ccggaaccga
     3841 ttgcgctgaa cgaagtagag ccggccaaag acatcgtcaa acgcttcacc accggcgcca
     3901 tgtcgttcgg ctccatcagc aaggaagcgc acgagacgct ggccatcgcc atgaaccggc
     3961 tgggcgccaa gagcaatacg ggtgaaggag gcgaggatcc ggagcgattc gagccgctcc
     4021 cgaacggcga ctcccgcaac agctatatca agcaggtggc atccgcgcgg ttcggggtca
     4081 cgagccatta tctggtgaat gcaaaagaac tccagatcaa gatggcgcag ggcgcgaaac
     4141 cgggcgaagg tggtcagctt cccggacata aggtggacga gaacatcgcg cggttgcgct
     4201 actccacgcc gggcgtgcag ctcatttcac caccaccgca ccacgacatc tattccatcg
     4261 aagacttagc acagctcatc ttcgacttga agaatgccaa tgccgatgcg gccgtctcgg
     4321 tgaaactggt ctccgaagtc ggcgtcggca cggtagccgc cggagtggcc aaggcccatg
     4381 ccgacaaggt gctcatcagc ggcgactcag gtgggacggg agcctctcca ctgtcatcga
     4441 ttaagtacgc cggtgtgccg tgggagttgg ggttggcgga aacgcatcaa acgctggtgc
     4501 tcaacgattt gcgcggccgg attcgtgtgg agacagacgg ccagatgaag accgggcgcg
     4561 atgtggccat tgcggccttg ttgggggcgg aagagtacgg gtttgccacg gcgccgctca
     4621 tcatcgaagg ctgcatcatg atgcggaagt gccacctcaa tacctgtccc gtcggcattg
     4681 cgactcagga tccggttctc cgcaagaagt ttaccgggca gccggagcat gtcgtgaatt
     4741 tcttcttctt catcgccgaa gaattgcggc agatcatggc gaagctggga ttccgcacca
     4801 tcaatgaaat ggtcgggcgc gtcgacaagc tcaagattca caaggccgtc gaacattgga
     4861 aggccaaggg gctggatctg gcgccgctgc tcaagatgcc cgaggtcggc cctgaagtcc
     4921 cacggtattg cgtgcagaag caggatcacg gcatcgccga gattttggac cgcgcgctca
     4981 tcgaacaatg tgcccccgca attgatcgtg gccaaaaggt cacgctcgaa ctgccgatcc
     5041 ggaacttgaa ccggacggtc ggcacgatgt tgtcgagcca ggtgtcgaag aagtatggtc
     5101 aggatggatt gcctgtcgac acgatcacga tcaagttcaa cggatcggcc ggccagtcgt
     5161 tcggcgcgtt tctctcgcgt ggcatcacgc tcatcctcga aggggagtcc aacgactata
     5221 tcggcaaggg cctatccggc ggcaagatca ttgtcttccc gccgaagaac gcgatttaca
     5281 caccggaaga aaccattctc gtcggcaaca cgtcgttgta tggtggcacg cagggcgaag
     5341 cctattttta cggcatggcc ggtgaacggt ttgccgtgcg caacagcggc gtgcgcgccg
     5401 tcgtcgaggg aaccggcgat cacggctgtg aatatatgac cggcggagtc gtggcggtcc
     5461 tgggcaggac cggtagaaac ttcgcggcgg gtatgtcagg cggggtggcg tttgtgttga
     5521 acgagctgga taagttccag tctcgttgca acttaggcat ggtggagttg gagccggttg
     5581 tcagcgatga ggacaagcgg ctgttgcacg atatgatcac gtcgcacttc ctctataccg
     5641 gcagccggaa tgccaagcgg attctggatg gctgggaggc gatcctgccc aagttcgtga
     5701 aggtcatgcc gatcgattac aagcgtgtgc tcgctgagcg gaaggccgct gcggccaaag
     5761 tgcaaaaata gatagcgcgc gtctggtgac gcgcacgtgt gagcaatcca gtcgatagac
     5821 gagtgacgaa ggacgagaga cgaattagcg atgggtgatc caaagggctt tctgaaatac
     5881 gcgcgtgagg ggccgaagcg gaaaccgatc gagttgcgcg tgctcgattg gaaagaaatg
     5941 tacgagccca tcccggagga gaagctcaag gttcaaggtg cccgctgcat ggattgtggt
     6001 gtgccttttt gccagggcaa taccggttgt cccgtcgtga atttgattcc cgagtggaac
     6061 gatctcgtct atcgcggacg ctggaaagat gcgctgaagg cgttgcacac cacgaacaat
     6121 ttcccggaat tcaccggccg tctctgtccc gcgccctgcg aaggcgcctg cgtgttaggc
     6181 atcaacagcg acccggtgtc gatccgcgtg ctcgaatgga acattattga tcgtggcttc
     6241 aacgaaggtc tggtcgagcc ggccttgccg gtgagaaaga ccgggaagac ggtcgccatc
     6301 atcggatcag gccctgccgg tctggctgcc gcgcaacaac tcgcgcgcgc cggtcatagc
     6361 gtcaccgtat ttgagaaggc cgatcgtatc ggcggcttgc tgcggtacgg catccctgat
     6421 ttcaagatgg agaagtgggt catcgaccgt cggctggagc agatgaaagc cgaaggggtg
     6481 gagttcaaaa ccggcgtcac tgtgggcaaa gatgttaccg gcgaacagct acggcaggag
     6541 ttcgatgcgg tggggctgac gatgggcgcg gaaatggcgc gagatctgcc ggtgccgggg
     6601 cgtgaactca agggcatcca tttcgccatg gagtatctca cgcagcagaa caagcggacg
     6661 gcagggatct ccgtatccga agaaccgatc accgccaagg gaaagcgggt ggtcatcatc
     6721 ggcggcggcg ataccgggtc cgactgtttg ggaacggccc atcgacaggg ctgcagtgag
     6781 gcgcatcagt tcgaagtgtt gcccgagcca cctccgtccc gatcgagctc gacgccctgg
     6841 ccgctgtggc cgatgcagct tcgtacgtcc catgcgcacg aagaaggctg tgatcgtcaa
     6901 tggagcgtgt ctaccacgaa gttcaccggt cacaacgggc atgtgacgaa gctgcatgcc
     6961 aaccgggtga agttcgaggg cggtaagttc gtggcgatcc cgaatacgga cttcgagttg
     7021 gatgccgacc tcgtgttgct cgccatgggc ttcacgggcc ctgttagaaa cggctttctc
     7081 gacagcctcg gcgtgaatta cgacgcgcgt ggctgcgtga ccgtcaacga gaacttcatg
     7141 accaacctcg acggcgtctt tgccggcggc gataccaagc gcggtgcctc cctcatcgtc
     7201 tgggccatcg ccgaagggcg caagatggcg gcggggatca atcagtatct gcaagcgaac
     7261 aagtctgcca agtcaggccg ctgaaaaagc ctcccggcgt cgttctcgct tccctcaaac
     7321 cctcaacgta ccgaaacagt acgcctcgcg ggctttcgct cgctgcggcc tagctgtata
     7381 ggcttttgga gcggccccgg gccgattcta atgtccttgg gcggtgcccc gctccagtct
     7441 gtcttatttt tgccccgcct tttaggatcg ctggtatttg tttgtctatc cccttgaatg
     7501 gtgctgcgcc ggctttcgaa cctatgtttc atgtcttggg ctacaatcga ttatgaatct
     7561 gcctaatgtt accgtgcgcg attcgcgcct ccgtacagtg ccggggctaa catgacgaaa
     7621 ttcgctcgtg ttccagacgc agactgggta ctagctggaa atgtcggaag tgcaagaatc
     7681 agccgcctca gacaagccga aaatttcgat gcggtcgtgt caaggatcat acgtggcgcc
     7741 cgggtcgaac gcttgaggcg agagcgttcg catgaagatc attcggcttt ccgcttgatc
     7801 gtggatcttc gcgtgaaggc ccaaagtctt gaccagtttt tcaattctcc tgttggctac
     7861 cgcgcgcagt acttcgcgaa ctgcaaactt ggcgagagag caaatcggtt cgtcatcaac
     7921 gagttgtttt cgaaggttct tgtggcagga aagagaggga gacgcctaaa ggaagatttt
     7981 attcgactgt cccttaagca ttcctctttc aaagttttgc tgcatcaagg ccagtggctt
     8041 cggcatgcga aacgcgaaga tcgagtcctt cttgttccca ggtggcaggc cgagtgcgtc
     8101 tcgggggaca aattacgacg gaaacttgct cgatggggat cgctggctcc agcgcacgaa
     8161 acgcgacttc tgctgaaggg aggcttcgta agggcgacca gcagagtaac tatcgataag
     8221 tctccaatga ctcgatctag agagcttcac gagcttggat ttacctagag aggcaggagg
     8281 tatgagtgag aaggaaagct agaattgact gaagcagtgc aatcaaaggg tcgcagctga
     8341 acagagtcca gaacgccttc catcgatcgt gaaaattgga agcagcaacg ttttgccagc
     8401 gctcatcggc cctcctagac ttctctcgtc ctcgcctgta tcttctcggg aattacattc
     8461 aggagaattt acaatgactc agcaaaccta tccccgtagc cccaaagtcc tcctcggcgg
     8521 catcgcccac ctgggccggt tcatcgataa agtccgcttg cgccacgccg gcaagattca
     8581 ggactacaac tacatcaccg tcgggttcga taagtatctg atcgattttt tacagatcga
     8641 tgcgaaggcc ttcgagcagc aggtgttggc gggcgagagc gatgagaagt tgttgagttg
     8701 ggtgaagacc cacggccgga aacattccga ggaagaaatc gcgcaatggt cgaaaggtct
     8761 tctggcgggc ggcccgaagg acgatgcggc gaagcagcga ttccagggac gggtgcagga
     8821 catcgccacc aagcgaggcg ttccgctgac ctcattgccg cctgtgacga cgtgggctga
     8881 tgtaatcgag ctggacgaag aacggatgta gccttacgac agtgaatcgt gggcgatgac
     8941 tatcgtactt cggtccagcc cgtggattgc gacttgaaga tcacggtgcg tgatgaggtg
     9001 cggacgacca tgccgttctg actgccatcg accgacagca cccgttcatt cggggtccag
     9061 tcaatgttga agaagcctcc cgtataggtc gaaaatccca gtgcccgctc attggtgatg
     9121 gcgacggcga cgtgcccttg gccgcgaagc tgtgagacgg tctcattcgg tcccagcgca
     9181 atggttgtcc agtgcccgcg cgtttcctgg aacccaaata cttgctggtt gctgtgcgcc
     9241 agaatcagaa acggcagtag ttgatgccgt ccgatccgtt cttctactcc aatcgacact
     9301 tcccgccatt gccgcaggcc ggcagaaaac cctaacaacc tgctcgaggt ctggacgaat
     9361 ccggtgtgtc cgcgtgctgc ggaactgatg accgtttcat tcggctgtaa ggattcttca
     9421 acgccgcccc ctgcagctga ccaggcaatg actttgctgt cggttggtgt gatggtgacc
     9481 tggctgcctc cgccttgagc gtgcgaaggc tcggcggtca cgagtagact tgcggttgaa
     9541 gtggttatga cgaacagcaa tagggcgata gatcgatgca acaggctggc tcccattcga
     9601 tgcgttaccc taacacatcg tcaggcataa gcgaagattg gacttgtgcg gggacgtgcg
     9661 tgaggtcagc ggtgcagagg aaaatgaagg aaaggcggga ccgtgcaacc ttgtcacggg
     9721 tcccgccagc tgcggtcatt acttatgctt tcggtttgac gttgagcacg acaatatcca
     9781 gcacgatgtg ttttcccgcc aggggatgat tgaagtcgac gacagccgac tcatccgata
     9841 gggatacgac ggtaaatggc cgaccttcag cagtcgtagc catatccccg cgggcaatgg
     9901 tgtgcggcaa ttgggcgcgc ggaacggtca tcttttttcc ggcgtcatat tgtccgaagg
     9961 catggtctgc atcaagttca acgcgcttgg tatcgcccga atgcaaacct acaagggcct
    10021 cttccaaccc tggaaggagt tgcttctccc catgggtgta ttcactggtt tggggaggga
    10081 tcatcacatg atcgttgggc agggtgatgg tcatttgcag tgtcactttt gaaccgtctt
    10141 cgatggttgg ggagcctgtt tgggcatgca gaggacggct cagcaggacg atagcacaca
    10201 ggatcagcaa gccggccaat gatacgcgtg tcatagatac ctcatctgtt gttagggata
    10261 gaatacaagg tgatcaactg acgattccgt tacggtgagt caatcttcac aattttggtc
    10321 acacggttgt cgaagtcaat ttccaggcta cacgtgtcgc ctttgtgtag accgctgagc
    10381 atgtcagcgg aaagggcggc cagagtccag acttcccctt cggcggtttt gaatgtcagg
    10441 ctctggctcg cttggtcgac ggattcaacc ttcacgcgcg tgtacgtcaa tgaagcctgg
    10501 ctctctaggg gagcaacaat cggcatggcg aggacacata ctagactgcc gatcacgatg
    10561 atcgatttca tgatgtcatc ctccttgtga caaaggcagg cgaacaatga ctcaccgctt
    10621 tcaaaccgcg ggcctgaatt gctactcaat gcatatggca gggagttatc atgttttcgc
    10681 tgggtaacgt cgcggaaggt tgacgagtgc ggacgaaccg gcgcgcaagt tgtccctgcg
    10741 ggacatattg tcctctcctc ggaccatgga aaagcagcgc aaatgtcgca ggatgatgca
    10801 tccttcccag gttcggaatc gggcggaata actgtcgcac tcgccttgtc tgcgatcgtg
    10861 acgcttccat tgatgggccc atggctcagc ggtcaatcgc ttcgacccta tctggagttt
    10921 ccgccgctca gcacgggtgt cgtgacgcag gtcgcatttt cctggccggt ttttgtcggt
    10981 ctgtccgcgc tgattgtctg cctgcttgcg ccgtttattg tgcgcgtggc acgaacgaac
    11041 agcaggggtg tatcgacggt agctgggcaa gagcgcacgc cgtatggctc cgttcgccgg
    11101 cagtgtgttt tgccccatgc ttttccctgg tggggttggg gtgctgtcgc ttggaccatc
    11161 tgtgcatggt tcctggcctg gacccgcttc gagtggatgc agccgtggca gccccatacc
    11221 ttcactccgc tgtggctggg ctacattgtc gtggtcaatg ccttgacgta tcgcagcacc
    11281 ggccggtgca tgttgatcca tcgtccacgg tatctgctct cactcttccc gctcagtgcc
    11341 tgcttctggt ggctctttga gtatctcaat cgatttgtgc agaactggta ctacgtaggt
    11401 ggcggaacgt tgacgacagg ggagtacctg gttcgcgcga ccctcccgtt tgcgacggta
    11461 ttgccggccg tcctcagcac ggcggagtgg ctcacggctt gcccgcgcct ctcggcggga
    11521 ttggaccatg ttgtcacgat caatttcagt cgccgggtat ggggatggtt gctgctatcc
    11581 ggcgctgctg ccggtctgct cggcatcggt ctgtggccgg actatctgtt tccgctggtg
    11641 tgggtggcgc cgctcctggt gatcacggcg ctgcaactga ttcgcaacga gccgacgatc
    11701 ttcagcgaga cggcgcatgg tgattggcga acactctggg cggtggcgct ggcagcgtta
    11761 gtctgcgggt ggttctggga gatgtggaat ttctacagcc tggcccattg gcaatatgcc
    11821 gtaccctttg tgcagggagt gaccctgttc cacatgccgt tgcttgggta tgccggttat
    11881 ctgccgttcg gcctggagtg ttgggccgtg gccgacttgt gtctcgccag gaagtttgca
    11941 ggaggcgtgg cctattatcg tgcggctgaa ccggagacgg atttcgcgca acggaacccc
    12001 acggtagcag aacgctgatc ggcgggtgcg ccgctgcgag ttgccgttcg cagcatgatc
    12061 gggcggcttt tccaggaacc accgcgaagg cttttgtatc ggccgctggt cgggccggga
    12121 ggattacgct ccggcatgta ggcgtaatag tcaaagccga accagtcatt gacccattca
    12181 gcgacgttgc ctgccatgtg atgcacgcca tagtagcttt tgcccggttc gccgctgctc
    12241 accggcgcca gaatcggaat ctcgtgcaca tgatgctgcc cgaacatggc cagggaattg
    12301 tcggggagcg actcgcccca gggaaaacga ttcccgtccg gcccccgcgc cgccttttcc
    12361 cattcggctt ccgtcggcag ccgtttgcct gaggcacggc agaggttgtc ggcttccttc
    12421 cacgtgacat acagggcagg ccactgcgcc aaggtctcat cgctgaccga gtgcacggtg
    12481 atgacgtgcc agatcaaatg ctggagatct ttggacgggt ggatctttcg ctgatgcaga
    12541 taggtcagat attccccgag gctgacttca tcacgatcga tctcgtaggc atccaaccag
    12601 acgcgatgct gcgggagctc cgtatcgtcg aactgtgtgc ccatgccata ggggtcgtcg
    12661 tccactcgaa tgctgccgag taagaacggt ccctcaggaa tgagaacggc cggtgagctg
    12721 gccgctaatt ccgcaatcga cgtcaggtgt ttggccagct ccgctgttgg cggaggggtc
    12781 ggggcgcgag cggcgatgga gagcgccgga ttgagccatg tggccgcaac gctcaggatc
    12841 acgagactga gcgcgtgcag gagtgaggcg attcgtggga acaacgtgcg agggatcatt
    12901 acaacttact cggcgaggcg acggcttttc cgatttcggc aatgagccgc cgttcgatct
    12961 ccgcgatctc ctcagcatcc accgccagga cgcgcccgcc ttgggctacc cgttcaaact
    13021 cggcttttac gctcaaccgg gtgacgttcg aggggagggc ttgaagggat accacatact
    13081 gattgcggga gccggccgtt tccaccgagg ttgggatgga aatttttcgc tcggtgacga
    13141 agaccgcttt ttgatcggtt ttgactgtga cgcgtatccg gtacccgttt cgcgtcaggg
    13201 cttcttccgc agctttagca acggccggca gcggacgcgg gagatcctcg acctgcgagc
    13261 ctttttcttc cacccggata ctgtccgtcg cctgggcggc tttggcggcg gtccgttgaa
    13321 tctctccgga gagttttttc gattcggcca gttgcgcatc gagcttcgtg ccgagcgtct
    13381 tcgcttcggt ttgcagcgac ttggcctcag cctgggcatg ctccgtctgc ttcttcgcgt
    13441 cccggagttc ctggttgagc agatcgatct gctgctgcat gactttgtta ccgtcctgca
    13501 gcgaattcag cagcgattcc tgcttcgcca gttgcttccg gagattttcg ttttcctgcc
    13561 ggaatggaga ttcgtcgggg gtacacccga cacaccacga tagtaccgcg aggctgaaga
    13621 ggggggcgag cgatcgctgg gaggccttga tcatagtttg ggtaagacaa tcagactagt
    13681 atcagagttg agcccccctt gtctatcttc aatcactttc tcgccggttt gaccatggga
    13741 aatggagcgg ctattctgtg ctcatgcggt actggtacga ggcggttttc ctcatgagtc
    13801 tggcattgtt tcagccggcc gcggcggcgg tttcgatcgc agcgatggct gaaccggcgg
    13861 agcccgtgcc tgccgaggac tatgcgtttt ttgacctcgc ggtcgaggac aagtttctga
    13921 cgtcccacac gaagttgatc gtcatcgaac ggatgacggc gacgcatctc cagccggatg
    13981 agccgcagct gccgaccctc gcctggtttg ccgagcgcga atggttcgat ggccgtttgc
    14041 cccaggacct ggtgcgggat ttcgtgggca agaatcagcg gccgtcgcgg cttgatacac
    14101 gatttagttt cggcgtgcgc taccggttta tgatgggcga aggaggcggc gatgccgaag
    14161 cctctgtgcc tctgattccc gccacctggt tagttcaaga agaaggcggc gcccccgacg
    14221 tcatcgacca tctggcattt tcgcgggtgg gtctgaacct gcgagccgat caggcgttgc
    14281 tgtatgtcgc gaatccgcgt cccgatggga ccggtgcggg ctttttgctg tggtttgtgc
    14341 ggcaacagaa ggtctggtcc ttgcacgaca cagaagtgtt gtgggtttca cggccggacc
    14401 aaggtcaatc aggccgccgg taggatgact gagacgttca ggttcctgtt cgattcactg
    14461 aggagtgcgc atgattcgta agacgttttc tgttccacca ctcgggtgta actgctcgat
    14521 catcggggac cccgtcacca agcaggccat cgtggttgat ccgggcggcg cgcctgagcg
    14581 catcctgcaa gaagtgcgtg cgatgggatt gacggtggtg agtattgtcc atacgcatgc
    14641 acacttcgat cattttctcg cgtccggcgc catgaagcag gcgaccggtg cggcgttgtg
    14701 tctgcatcct gacgacctgc cgctctggga cattttggag atgcaatgcc gcatgttcgg
    14761 cgtgccctat gttccggtgc cgcagccgga ttattggctg aaagatgagg agcgtgtggc
    14821 cgtcggcgag atggaaggcg tggccttgca cacgccggga cacacgcctg gatccatgtg
    14881 ttttcacttt cccgctgcaa aattgttggt ggcgggggat acgctgtttc gcggtggcat
    14941 cggccgcacc gatttgtggg gcggggattt cgatgccatc gagcaatcga tccgggaacg
    15001 gctctacacg ctcgatgcgg acacggccgt cgtaaccgga catggggctg agaccgagat
    15061 cggccttgag cgagagacga attcattcgt gcgcgcctag cggagaggtg ggcatttcac
    15121 ggtgcgtgaa ccttctgcgg ggcgcgagat cgttccccga cagcctttgt catagccgct
    15181 catgaacggg cctccatcgg aaacacagga gggtattatg atcgcgacgg ctcggcgctg
    15241 gttggtatgt ccgctgatgt ccttcctgct gggattctcc gctgccgatg tgctcgtgta
    15301 tgccgacgaa cctgggtctt ctccccaggt cacgatcagt caggtccggg tgggattgtc
    15361 cgatcacggc ccggtggtgt tgctatcagc cgacgggaaa accattcccg tctttgtcga
    15421 ccacaccgtc gccgcgtcca ttcaggccgc actcaccggt gaaaagcttc cgcgcccgct
    15481 ttcccacgag ctgatgcata cgattttaga atcgctcggc ggacgggtgg tccggacggt
    15541 gattacgctc aaggcgggca cctattacgg aagcctgacc gtggcgtttc aggggcagga
    15601 aaaggtgttc gatagtcgtt cttcggactc catcgccctg gcgattcatt ttcacgcgcc
    15661 gattctggtg gcgcgggaca tgctggatgc ggtgggcaag tcctcgctgg agccgaagcc
    15721 cgaatcactc tgagctcgcg cccgacggaa catcgcggga ctctcgcgag tcgcgtagaa
    15781 gtatacagtg ctggcctttt gtccggttct atcctatcgt actgctgatg cgcagaggcc
    15841 tgaggttgtc acaggtacgg aggccacgtt tccatgaaaa accaacgcaa gcatgagcgg
    15901 gtgcacgtcc agtttcgcag tcatttttcg atgaaaggca agatgctggc cggtgatgga
    15961 gatctcaccg atctctctcc gggcggctgc cggattatca gccctgtcga agtgctggtc
    16021 gggtcggaag tggagctctg tattttcccc ggtgacaatg ccaatcccat cctcatcgat
    16081 gcggccaccg tacggtggtg ccgacccaac gagttcgggt tgtcgatcac caagattcga
    16141 gggccggtcc agcgccaatt gaccgacgtg tggcgcaagt tggcgaagcc tgcttagcag
    16201 gctgttgata caggcgcgcc agcggcgttc tcacctcgct cagaggctca ccgtacggcg
    16261 cgagtacgat tcgcctcttc gctcgcttcg gccttgctgc acggcctttc tgaacagcct
    16321 gcacggcgcc agacgcaact gcgcatttca cgcaacgatc ctcttccgga tgccgcgtac
    16381 agttgatcaa cgcgctgcta gtcgagtgtt attccagaag tgagttgccc aaatcggata
    16441 ggcgtggcta gatcttcatg gcctcccgca cttcggccat cgtctgagaa gagacggcgg
    16501 tcgcgcggcg tcgcccgtcc tctgcaatgt catcaaggcg ccccggatgt tgcgtgaggt
    16561 tcgcacgagc ctcccacatc ggagccaggc gctccaccac gcgatcggcc accagcttct
    16621 tgcaatcgat gcagccgatg gcggctttcc ggcaatcctg attcacctgt tcgatcaccg
    16681 gcagcggcga aaaaatcttg tgaaagtcgt agacggggca gacgtcggga ttcccggggt
    16741 cggtgcgccg gacgcgcgcg ggatcggtga tcatggtctt gagcttctgg cggaccaccg
    16801 cttccgtatc ggagaggttg atggcgttgc cgtagctctt gctcatcttg cgtcc
//
LOCUS       DAOQEW010000071        16830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_904_length_16830_cov_8.721371, whole genome shotgun sequence.
ACCESSION   DAOQEW010000071 DAOQEW010000000
VERSION     DAOQEW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_16830_cov_8.721371"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1533)
                     /locus_tag="PLS35_09450"
     CDS             complement(205..1533)
                     /locus_tag="PLS35_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18930.1"
                     /translation="MPVDSDSLAAFRKQVTSARSLDAPLWEASRHLVHSADWPATRTA
                     LVKAIHASSIPDGLKQTLVAALPVDLATEDRTSSAVLLKQLTGLPLTKALRALCVFFQ
                     VGRPAMAKWPLPTVTPDTIDRFVQTHHNPFDVLLEQLPASLLDLGSGDLSFAEELATI
                     YDPPLTAQHRPLLLHCLDRLDPSSQLGGPLHAPPLRLNRLRSKPGLEFRFYGDQDMFA
                     LDSLERDQRLAARYLIVTCWAPATPTFAYEPTRLSARCLAEELRRTKGDSRQIRHGKE
                     PALEVSHAGRNLLFPPWKFDIRGPLALLELMASRAALCVLGAVDSQVCWEILSQLIDD
                     PRVRPADVVLSAETLPAIFGETYRQLSALPVGGSCLLSDLTPLRKSFPSVLHTSPGHP
                     APYRFRQVKIQRGALFAGLPASSTARRFQGMSEETPPWLLTLVPEPVAIS"
     gene            1667..2683
                     /locus_tag="PLS35_09455"
     CDS             1667..2683
                     /locus_tag="PLS35_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNI18931.1"
                     /translation="MRTSLQHLREKVLNNLSGVIAILVLVVALGGCQAAGPVPRVTPQ
                     VWDAWSVGQILDAKTGHVVSTAEWLEGLAGYDVIYLGEEHHNRSHIESALTVLRTLVT
                     RGRRPVLAMEMFGWEGQPALDAYLETKESARPEFLERVGWKQNWGGAFEDYEPLVQFA
                     KDQRLPVLAMNPPKTLIRQVVKLGLAQAKEQPEWRQRGMEQETIVDDPAYRSRILSQL
                     QACHGGGAPEDYQTMYDASMVRDEGMAKTVAAAVNRVRAAGDPTQGPVVSYTGGGHVQ
                     YRLPVPNRVARRVEHGLKQVTIYMATLEQDRAAELYQSMQEGIADFVWLTPPGSQGAP
                     RRCR"
     gene            2954..3766
                     /locus_tag="PLS35_09460"
     CDS             2954..3766
                     /locus_tag="PLS35_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18932.1"
                     /translation="MISSIVEPNAFQIGKQYQTERRVQLTDASDTEFTSSVMGNSGLY
                     EQTIRLHNGHLEAKCSCTLHEQPICRHGVAALLEYQRWSKARVVPRARPAAPRLEAER
                     ATTKSAPSGDVKLSELNQFSDWMQRVVQAIQSGQPVPAQPDIEPGLVSTWTRIIQQLD
                     ERRRESEVAQIELDTDIRNREAVVARLTQDLEASAKEAKSLQVICRDLQREVDQHKAS
                     LSKSTELSRHVEQFEGEVRAIAGLLTEKGRRLEGLSDTCRDVATMLKALGKV"
     gene            3908..4084
                     /locus_tag="PLS35_09465"
     CDS             3908..4084
                     /locus_tag="PLS35_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18933.1"
                     /translation="MNKLIAGVLGGIGALQATNALANVSEGPPDYSGITGLYYVLIAL
                     ILAFGVYDTFFKKS"
     gene            complement(4147..4719)
                     /locus_tag="PLS35_09470"
     CDS             complement(4147..4719)
                     /locus_tag="PLS35_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNI18934.1"
                     /translation="MAFGNSLTAGLGVSPEHTYPAQLQKQLDTLGLHYQVLNAGVSGD
                     TTAGGLRRVSWVLAGKPSVVILELGGNDGLRGLSLPETRSHLDTIIKRLKEARVQVIL
                     AGMKLPPNYGEEYTTQFEAMYRDLAKAHALALIPFLLEGVGGDQKLNQADGIHPTGEG
                     YRIVVQNVLQTLLPLLKSGESHSPTSKKKA"
     gene            5008..5715
                     /locus_tag="PLS35_09475"
     CDS             5008..5715
                     /locus_tag="PLS35_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18935.1"
                     /translation="MMIATQQVTMQLEAGGETVRILDDITLEIPEKQTVAIVGPSGSG
                     KSTLLGLIAGLDRPTSGTIWLNGREITRLNEKAMARLRLANVGYIFQSFHLIPTLTAL
                     ENVSIPLELAGDRHARQRAMELLEAVGLGHRVSHYPVQLSGGEQQRVAVARAFACRPP
                     ILLADEPTGNLDSHTGQQVIQLIMALHRDAGTTLVLVTHDQQLAASMERVITLRDGRI
                     GSDQFMASSYDESGDRL"
     gene            5712..8384
                     /locus_tag="PLS35_09480"
     CDS             5712..8384
                     /locus_tag="PLS35_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI18936.1"
                     /translation="MRPFWFILAWRDLRSSWRHFVYFLACIALGVGAVVGVSLFSANV
                     ERAVLKEARGLLGGDLEIRLSRPLGGPGSTVLKDLTGRGIEVTRVDELVAMVARVDRR
                     EGGMDVTQLVELKAVEPRYPLYGVVRVEPDRPLTELLHPAGEPCVTICHGAVVQQALL
                     IRLGLGVGDAMKIGQASFRITGVIHTEPDRMANMFSLGPRVLISQEGLAAADLIKPGS
                     RMRERHLLRLPASMALSPLLHELRGRLGVESARVSSYRDAQPQLKQFLDQLARYLGLV
                     GLTALFVGGIGVALSIHAFVREKLPSIAILKTVGADTATIIYSYLGQAVGLGLLGSLG
                     GIGIGVALQTVLPQAVSTLLATDVLQQVEFTSVLSLSAVAPIGKGLGLGILTTLLFSV
                     WPLLTIRDIKPAAIFRREVERVGAPLVRREKALWKRAGQVMTADPLRTVTAAGIGIGL
                     ASLSMWQAGSLTIGALFIGGLVIAIAVLTLGAQALLRGLRALPVPRELSLRQALGNIQ
                     RPGGQTLGVMVSIGVGVMVILAIALLQHALVRQVGENRPSDSPTFFFIDIQPDQAQAF
                     GELVHRRTGDFAPDLTPLVRSRIHAINGRPVTDEREAQQDEPPAQSKEEKRKSWYVTR
                     EYVLTFLDQVPKDNKIVKGAWWKPGQVFSKPQVSVEEEAAKSLDLDVGSLLDLNIQGT
                     ILQAEVSSIRKVEWGNFSTNFYMIFSPGSLDAAPMTYVATVRVAPQDEVAFQSAVVAA
                     FPNVTAINIGEVLNSFARVLDRLSLAIRGVALFCLMAGALVMAAALAATRYRRLYEAV
                     ILKALGATRGLIARSFAAEYALLGCVAGMIGVALASLFSWAILRYILELPWALELPVL
                     GIGLGCTVLLTLLVGFLSTYRILGQPPLTVLRHE"
     gene            complement(8413..11187)
                     /locus_tag="PLS35_09485"
     CDS             complement(8413..11187)
                     /locus_tag="PLS35_09485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNI18937.1"
                     /translation="MTRPQGDLPVTRRQAAALAVRAATHAALWLGAWPGIASLVFVPE
                     ARSQTTSTHNQQHPHNTMTTSSAIADPYRLPRHVIPTYYDLRMEPDLPSHSFTGHEIV
                     TLTVTEPTTDILLNATELEVSAATLSGEGQPPRTGTVRMDDEHQRCHITFPAVIPPGV
                     WKLSLAFRGTLNDKLRGFYRSSYKDDRGIAHSLAATQFEATDARRAFPCWDEPHFKAV
                     FAVTLAIDPALTAISNTRIVDERLENGKRIVRFAESMKMSTYLVAFIVGELVSTAPIM
                     AKQTPVRLWSVPGKQHLTPFGHEIAVYSLNFLAEYYDIPYPGDKLDLIAIPDFASGAM
                     ENLGAITFRETALLLDQRTATHAEQGRIADVVAHENAHMWFGDLVTMAWWNGLWLNEA
                     FATFMEMLVVDAWKPEWERWTAFGMARAAALSVDGLLSTRPIEFPVRAPKEAEAMFDV
                     LTYEKGASVLRMLEQHIGPTVFRDGVRHYLHAHAYGNAETTDLWVSLGHASKQDVPAL
                     MNEWIFSPGYPLLSLSVESRSTLMLTQRRFMYADDSSTAPSGTASPRWQVPVQLRINT
                     ARGTETRRVLLSERENRIPLPEGWTSILANEGGHGFYRVRYSADLLSALQKDGLNTLT
                     AVERFNLLNDTWAATVAGMVSSADYLALTEHFRSEQDPHVWAVMLGSFSTINHLLEEE
                     DRPLLAALVRDRVAPLFQQLGWSPHADERDLVKELRGDIIRALGTLGRDETIQAQARE
                     AYTAIQQKSRPIDPNVIPALVSILAFTGDEARYEEFSGRFRQATTPQEERRYLFSLAA
                     FRRPELLERTLTKTLTDEIRTQDAPFLVSSLLQNVYIREKAWKFVKSNWERMDKLFPK
                     SGLRRMCGGLTGLSTPELEQDVRHFFTSRKIDLGGKTLEQNLEQLHIAVQFREREREA
                     IRAVLARRST"
     gene            complement(11184..11888)
                     /gene="rpiA"
                     /locus_tag="PLS35_09490"
     CDS             complement(11184..11888)
                     /gene="rpiA"
                     /locus_tag="PLS35_09490"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNI18938.1"
                     /translation="MTTTSCDLDSEKRQAALKATEFVHDGMVVGLGTGTTSKHLIIAL
                     GERVRAGLNIQAVPTSHDTAALAKQSGIPLIESDNAWMIDVAIDGADQVDPALNLVKG
                     GGGALLKEKIVAAAAKRFIVMVDHTKLVPALGGSFPLPIEVVPFGWGSTARHIEAVSG
                     GRAVLRQRNGDVFQTEAGHVILDLHMPIIDDPAALEVELNQIPGIVETGLFIGRTSIL
                     IVGHGQGTDVTHAPKP"
     gene            complement(11885..12970)
                     /gene="glk"
                     /locus_tag="PLS35_09495"
     CDS             complement(11885..12970)
                     /gene="glk"
                     /locus_tag="PLS35_09495"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HNI18939.1"
                     /translation="MILAGDIGGTKTNLALYEWTSGRVEPVREDSFHSADYKSLEEII
                     DEFLSTPLVKPALENALTEESVDLGSDVAAETAPVVPEPITLTAACFGVAGPVIDNRC
                     RTTNLPWFIDGAALGERFVIPHVRLLNDLEATAHGLLHLTTDEMVVLNAGAPPKKKQA
                     LALIAAGTGLGECILYWDGTRYRPMPSEGGHTDFAPNSDSEIELLRHLRGSYLHVSYE
                     RIVSGPGLHAIYEYLRDTKKNEPTWLAEKIKVGNPAAEIAEAGLKGQAEIATQALELF
                     ASVYGAEAGNLALKALTLDGVYVAGGIAPKLLKKLQDGSFMRGFTNKGRYKRLMNQIP
                     VKVVMNDKTALLGAASVAAQLTLPPSL"
     gene            complement(12979..13749)
                     /gene="pgl"
                     /locus_tag="PLS35_09500"
     CDS             complement(12979..13749)
                     /gene="pgl"
                     /locus_tag="PLS35_09500"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNI18940.1"
                     /translation="MAHPPERYIFADAQEVVRAAATLFVTVGQQAISKRARFLVALSG
                     GSTPKALYMVLASKEFATQLDWNKVHFLFGDERAVPPTHQDSNFALANTLLFTPLNIS
                     ATHIHRMRGEDSPETAAIHYENLLRGLTATTSEQWPVLDLVLLGMGEDGHTASLFPGT
                     PALSEPTRWVVPGLAPQGTRSRITLTLGVINHASVVLFLVTGLNKATVVRRIFESRAD
                     APDAYPAARIRPETGRLLWYFDRAAASALTEATDELSS"
     gene            complement(13751..15451)
                     /locus_tag="PLS35_09505"
     CDS             complement(13751..15451)
                     /locus_tag="PLS35_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNI18941.1"
                     /translation="MPIRPKVTSLLDTQTLATTFDELASSRAIERILMRDHTLWKPNP
                     TEIDNRLGWLTVLDHMQDGLSDLRSFAQAAREARTTDVVLLGMGGSSLGPEVLRCTFG
                     SSKGAPRLWVLDSTVPGWVRQVTDAIQPARTLFLVASKSGGTIEVMSLFAHFWELVHR
                     TKGHRGGAQFVAITDPATGLEELARERGFWRTFTNQPDIGGRYSVLSYFGLVPAALLG
                     LDVGKLLTRALAMRESCRHTVAKENPGSALGGLMAAMAKSGRDKVTLLTSPALTSFGL
                     WVEQLLAESTGKEGTGLIPVAGEPFASPAAYGADRIFISLRLRGDRNASLDRTVSALQ
                     RAGQPVYSIPLKDRYDLGGEFFRWEFATAIAGHALGIHPFDQPNVQESKDNTGRVLKE
                     VETQGKLPSIAVLPPKQALTQLLEQMGSNRYLAILAYTTPTTQVEAALRALRKALMVR
                     YHVATTTGYGPRYLHSTGQLHKGGPNSGLFLELVDTMKPDLAVPEKFYTFGTLAQAQA
                     LGDLQSLHTHQRPAVRIALGPNPVRTIRSLVTFLTPAKATRRKSVPKKRTTSTRRSRR
                     "
     gene            complement(15639..16790)
                     /locus_tag="PLS35_09510"
     CDS             complement(15639..16790)
                     /locus_tag="PLS35_09510"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNI18942.1"
                     /translation="MMSRSRYICRIATRVTLVALLSMSLGGCVETVPEELVESIESLD
                     RDLSTLRASDVNPAAYSKFSRQWIALQGRIRSEENIVRWPWEDNELETDLEALQVEGT
                     QLLSDVNSRIQAQRTTAETKLAKIEQRLRLLNSRVGDIGSRVVLGEHPVETEVHVKQA
                     RLFLEQGQFEHSIQASERAGRILLTQTALLTNELGRYADDDLIAAWRESAQRTIDWSR
                     THRAQAIVVSKADRSLTLYKNGRKVLTYPIRLGSRGIRAKQHYGDGATPEGEYRIQRK
                     RGPGQTPYFRALILDYPNIDDRRRFEEAQKAGLIPKSQHMPGLIQLHGIAQGISDQPY
                     GSIVLDNPQIAQLFDQVAVGTPVNIVGALESQNSISLVLADLGDQEEET"
BASE COUNT         3308 a         4609 c         5283 g         3630 t
ORIGIN      
        1 acctgcgcga tattgtcctg taacagtttg atagattgag aagaattcac gatgggtttg
       61 tttgtgaagg gggttatgtt gggcacatgg cgtgtatgcc cgaagtctaa caaagggtct
      121 ggagaccgtc aatttatacg gcctttcatg cggggtggct cggtgatcga tttgtgaggc
      181 gccagagcgg gaaccaggcg agtatcagga aatggcgacg ggctcgggta cgagcgtcag
      241 caaccagggg ggcgtttcct cagacatgcc ttggaaccgc ctcgctgtgc tactggcagg
      301 gagtcctgcg aatagcgcac cgcgctgaat tttgacctgc cggaatcgat agggggcagg
      361 atgaccggga gaggtgtgca gaacagacgg aaaactcttc cgcagcggcg tcaggtccga
      421 tagcaggcag gagccaccca ccggcagagc tgagagttgg cggtaggttt ccccgaagat
      481 cgctggcagc gtttctgccg acaacacgac atcggcgggt ctgacacgcg ggtcatcgat
      541 cagttgcgag agaatttccc aacacacctg cgagtccacc gcccccaaca cacagagggc
      601 tgcccggctg gccatcaatt ccagcagggc gagcggccca cgaatatcga acttccacgg
      661 tggaaacagt aagtttcgtc cggcatggct cacctcgagg gccggttctt tgccatgtcg
      721 aatctggcgg gaatctccct tggtgcgtcg gagctcttcg gcgaggcagc gggcagagag
      781 gcgagttggt tcataggcaa aggtcggcgt agccggggcc caacaggtga cgatgagata
      841 tcgggcggcc aagcgctgat cccgttccaa agagtcgagg gcgaacatgt cctgatcgcc
      901 atagaagcgg aactccagac cgggcttgct gcgcaggcga ttcagtcgaa gcggaggggc
      961 atgtaatggt cctccgagtt gggaggaagg atccagccgg tcgaggcagt ggagtaggag
     1021 tggtctgtgt tgcgcggtca acggggggtc atagatggtg gccagttctt ccgcaaacga
     1081 aagatcccct gatcccaggt ctaacaacga ggccggaagt tgctccagta acacatcgaa
     1141 tggattgtga tgcgtctgga cgaatctgtc gatcgtgtct ggcgtgacgg tcggcagggg
     1201 ccatttggcc atcgctggtc gtccgacctg aaagaacacg cataaggccc ggagagcttt
     1261 tgtgagggga agccccgtga gctgcttgag gagcacagcg gaagaagtgc gatcttcagt
     1321 cgcgagatcc actggtaacg ccgccaccag agtctgcttc agtccgtctg gtatggaaga
     1381 agcgtggatc gccttgacga gggctgtcct ggttgcgggc cagtctgccg aatgtacgag
     1441 gtggcgcgag gcctcccata acggcgcatc gagcgatctg gcggatgtga cctgtttccg
     1501 gaatgcggcc aggctgtcgg aatcaacagg catcaggagt caacgaccgg cgtgtgggtt
     1561 gtgggtgatc gaccgttccg ttgcgtcgcg cagagtatcg ggccggttgt aaaggagtca
     1621 agcaggtcgt cttgcggctg catgatgcgt atgttaggta gacgatatgc gtacatcatt
     1681 acagcattta cgtgagaagg tcttgaacaa cctcagcggt gtcatcgcga tattggtttt
     1741 ggtggtcgct ctgggaggct gccaggcggc cgggcctgtg ccccgggtaa ccccgcaagt
     1801 ctgggacgca tggtctgtcg gtcagatact ggatgccaag acgggccacg tcgtttcaac
     1861 ggccgagtgg ttggaagggt tggccggata tgacgtgatc tacttgggtg aggagcatca
     1921 caatcgatcc cacatcgagt ccgcgctgac ggtgctgcgg acgcttgtga cccgcgggcg
     1981 tcggcctgtg ctcgcgatgg agatgttcgg ctgggaaggc cagcccgctc tggatgccta
     2041 tctcgagacg aaggaatccg ctcgcccgga atttctcgag cgtgtgggat ggaaacaaaa
     2101 ctggggtggc gcttttgagg attatgaacc gctcgtgcag tttgccaagg atcagcggct
     2161 cccggtgctg gccatgaatc ctcccaaaac gctgattcgt caggtcgtca agctgggact
     2221 ggcccaagcc aaagagcagc ccgaatggcg tcaacggggc atggagcagg agacgatagt
     2281 cgatgacccg gcctatcgct cccgtattct atctcaactc caggcctgcc acggaggggg
     2341 ggcaccggaa gactatcaga ccatgtacga cgcctcgatg gtgagggacg agggcatggc
     2401 gaagaccgtc gcggcggcgg tgaatcgtgt tcgcgctgcg ggcgacccga cacaggggcc
     2461 ggtggtgagt tataccggcg gcggacacgt ccaatatcgg ttgccggtac cgaatcgcgt
     2521 cgctcggcgt gttgagcacg gactcaagca ggtgaccatc tatatggcca cgttggagca
     2581 ggaccgggcc gcagagctct atcaatccat gcaggaaggt attgcagatt ttgtgtggtt
     2641 gaccccgcct gggtctcaag gggctcctcg ccgatgccga taagatatcg ggcggtcgag
     2701 tcctcgcggc cctgccgtat tctgtcgacc cctgcagggg gctccgtcac cggaatggtc
     2761 ggatcgggga acctgccgta cactcgggtc ccatgcctgg atgatgggcc tcaggggtaa
     2821 gcattccatg acactgcggc gatcattcga taagttgcaa gggcgactca ctggaaccaa
     2881 ccattccaca ggtggcacga tgcgggggaa aatcgcatgt tcggcggatc gcctcagttt
     2941 actggaggcg gacatgatca gctccatcgt ggagcccaat gcgtttcaaa tcggaaagca
     3001 ataccagacg gaacgccggg tgcagctgac cgacgcctcg gataccgagt tcacctcctc
     3061 tgtgatgggc aattccgggc tgtacgagca gaccatccgt ctccataacg ggcacctgga
     3121 ggccaagtgc tcctgtaccc tccacgagca accgatctgc cggcacggtg ttgctgcgct
     3181 gctcgaatat caacgatggt cgaaggctcg cgtggtcccc agggcgcgac ctgcagctcc
     3241 ccgtcttgaa gccgaacggg caaccacgaa atccgctccc tccggggatg tgaaactcag
     3301 cgagttgaat cagttcagcg actggatgca acgagtggtg caggctattc aatccggtca
     3361 gccggttcct gcacagcccg atatcgagcc gggcctggtc tcgacctgga cccggatcat
     3421 tcagcaattg gatgaacgac ggcgggaaag cgaagtcgcg caaattgaac tcgatacgga
     3481 tattcgcaat cgtgaagcgg tcgtcgcccg cctgactcaa gatttggaag cctccgcaaa
     3541 ggaagccaaa tccttacagg tgatttgccg ggatctccag cgggaggtgg atcagcataa
     3601 ggcctccctg tctaaatcca cggagttgtc acgacacgtc gaacaattcg agggcgaagt
     3661 gcgggccatc gccggtcttt tgacagagaa aggccggcgt ttggaaggcc tctccgatac
     3721 ctgtcgagat gtggccacga tgttgaaagc cctggggaag gtgtagcggc tacttccgcc
     3781 ttctaaccgg ccctgtctct tccaattccg aatcgaatcc ttctttctgt ccccccttgc
     3841 gtcatcccca gtccgttcag tacaatgcgg gcctctttgg gcattccttg actacaaggg
     3901 ggagatcatg aacaagctga tagcaggagt tttgggtgga atcggggcgc tccaggcgac
     3961 caatgccttg gccaacgtca gtgaagggcc accggattac agtggcatca cgggcctgta
     4021 ctacgtcctg attgctctca tcttggcgtt cggggtttac gacaccttct tcaagaagag
     4081 ctaaccagct cctcaggatc aagtggcgtg aaacgtttct ccgttgcgga aggcggaggg
     4141 gatgtttcac gccttctttt tgctagtcgg ggaatgggac tcgccggatt tcagcagcgg
     4201 aagcagggtt tgcaacacgt tctgaacgac aatccgataa ccttccccgg tgggatgaat
     4261 gccgtcggct tggttgagct tctggtcgcc gcccactcct tccagaagaa acggaatcag
     4321 tgcaagcgca tgggccttgg ctaggtcccg atacatcgct tcaaattgtg tggtgtactc
     4381 ctcgccgtag ttcggtggca gcttcatgcc ggccagaatc acctgtaccc gcgcctcttt
     4441 caatcgttta ataatcgtat ccaggtgcga ccgagtttcc ggcaggctca agccgcgcaa
     4501 tccatcattt ccccctaatt ccagaatcac gacggacggt tttccggcga ggacccagga
     4561 tactcgacgc agtcctcctg cggtcgtgtc tccgctcacc cccgcattga gcacctgata
     4621 gtgaaggccg agtgtatcca gctgtttctg cagctgcgcc ggatacgtgt gttcggggga
     4681 gacgcccagg ccggcagtca aactattgcc gaatgccacg attctcggtc ggctgtcggc
     4741 ctctggcacc gtcgtcgtag ccggcgggcc cgctgaaggc ttgtcgtcag tcgccggctg
     4801 aggtggggca aggctggcag gcgcggatga tgagggagga ctagaggcca ggggagattg
     4861 atcgcagccg acgagcccgc tcacgagcag gacgataagg ctgcctgtca tcagtcgcgg
     4921 gatgatgcgg aaaaggtccg agcaggtcgt catgcgtaca gtataatatg aaaactgttt
     4981 ttacatgtgg gtctacggat caacaggatg atgattgcca ctcaacaggt gacgatgcaa
     5041 ttagaggccg ggggcgagac ggtcaggatt ctggatgata tcacccttga gattccagag
     5101 aagcaaaccg tggcgattgt cgggccgtcc ggcagtggca agtccactct gctgggcctg
     5161 attgcggggc ttgaccggcc gacgtccgga accatctggc tcaatggccg ggagattacc
     5221 cgactgaacg aaaaggcgat ggcgcgcctg cgcctggcca acgtgggata tatctttcag
     5281 tccttccatc tcattccgac gttaaccgct ctggagaacg tgtcgattcc gttggagctc
     5341 gcaggcgacc gtcatgcacg ccaacgcgcc atggaattgc tggaggcggt aggcttaggg
     5401 catcgggtct cgcactatcc ggttcagctt tccggtgggg aacagcagcg ggtcgcagtg
     5461 gcgcgtgcgt ttgcctgccg gccgccgatt ctgttggccg acgaaccgac cggaaacttg
     5521 gactcccata cggggcagca ggtgatccag ctcatcatgg cgttgcaccg ggacgccggc
     5581 accaccctcg tgttagtcac acacgatcaa caactagccg cttcgatgga gcgggttatt
     5641 accttgcgag acggccggat cgggtctgat caattcatgg catcttccta cgacgagtcc
     5701 ggcgaccgcc tatgaggcca ttctggttca tcctggcctg gcgggacttg cgatcttcct
     5761 ggcggcattt tgtctatttt ctcgcctgta tcgcattagg cgtcggggcg gtagtggggg
     5821 tgtctctctt ttcggccaac gtcgaacgcg cggtgctgaa agaggcacgg ggcctgctgg
     5881 gtggtgatct ggaaatccgg ctatccaggc ctttaggggg accgggctcc accgtgctca
     5941 aggacctgac gggccgcgga attgaggtca cgcgtgtaga tgagctggtc gccatggtgg
     6001 cgcgggtcga ccgtcgcgaa ggcggaatgg acgtcacgca gctcgtcgaa ttgaaagccg
     6061 tcgagccgcg atatcctttg tatggtgtcg tgcgggttga gccggatcga ccgttaaccg
     6121 aattgcttca tccggccggt gaaccttgcg taaccatctg tcacggggcg gtcgtgcagc
     6181 aagcattgtt gatccggctg ggcttaggcg ttggtgatgc gatgaagatt ggacaggctt
     6241 ctttccggat cacgggcgtg attcatacgg agccggatcg catggccaac atgttcagcc
     6301 tcggcccgcg ggtgctgatt tcgcaagaag ggctggccgc cgccgatctg atcaagccgg
     6361 gcagccgaat gcgtgaacgg catttgttac ggcttcctgc ctctatggca ctgtccccgc
     6421 tccttcatga gctgcggggc cgccttggcg tcgaatccgc gcgggtgtct tcttatcgcg
     6481 atgcccagcc acagctcaag cagtttctcg atcaactcgc gcggtattta ggcttggtcg
     6541 ggctgacggc gctgttcgtc ggcggcatcg gcgtggcgct atccattcat gcgttcgtgc
     6601 gagagaagct cccctccatt gcgattttga aaaccgtggg ggcggatacc gcaaccataa
     6661 tctattccta cttagggcag gccgtcggct taggcctgtt gggtagcctg ggagggatcg
     6721 gcatcggcgt ggccttgcaa acagtccttc cgcaggcggt gtccacgttg ttggcgacgg
     6781 atgtcctcca gcaagtcgag tttacatcgg tgctctctct ttcagccgtg gcccctatcg
     6841 ggaaaggctt gggtttgggc atcctgacga cgctgttgtt cagcgtgtgg ccgctgctga
     6901 ctatccgaga tatcaaaccc gctgccattt tcaggcgtga agtggaacgt gttggtgcgc
     6961 cactggtgcg acgagagaaa gccttgtgga aacgcgcggg acaagtgatg acggcggatc
     7021 ccctgcgaac cgtcacggcg gcgggtatcg ggatcgggct ggcgagcttg tctatgtggc
     7081 aggcgggatc gctgacgatc ggcgcactgt ttatcggtgg cttggtgatc gcgatagcgg
     7141 tcttgacctt gggagcccag gcattgttgc ggggactccg cgcgttgccc gtaccgcggg
     7201 agttgtcttt gcggcaggcg ctgggcaaca ttcaacgacc cggggggcaa accctaggcg
     7261 tgatggtgtc gatcggcgtc ggcgtgatgg tgatcctggc gatcgcgttg cttcagcatg
     7321 cgctcgtgcg gcaggtggga gaaaaccgtc cgtccgattc tcccaccttc tttttcatcg
     7381 atattcaacc ggatcaggca caggccttcg gggagttagt ccatcggcgc accggcgatt
     7441 ttgctcccga tctgactcca ttggtgcgct cacgaatcca tgccattaat gggcggccgg
     7501 tgactgacga gcgcgaggct cagcaggatg aaccgcctgc tcagtcgaag gaagagaagc
     7561 gcaaaagctg gtatgtgact cgtgagtatg tgctgacgtt tctcgatcaa gtgcccaagg
     7621 ataataagat tgtgaaaggc gcctggtgga aaccggggca ggtgttttcg aaaccgcagg
     7681 tgtctgtcga ggaagaagcc gccaagagtc tcgatcttga tgtcggcagt ctgctggatc
     7741 tcaacattca aggaaccatt ctgcaagccg aagtgagcag catcaggaag gtcgagtggg
     7801 ggaacttttc cacgaatttc tacatgatct tttcgcccgg ctcgctcgat gcagcgccga
     7861 tgacatacgt cgccaccgtg cgagtggctc cccaggacga agtggccttc cagtcggccg
     7921 tggtggctgc gtttcccaat gtgaccgcca ttaacatcgg cgaagtattg aacagctttg
     7981 cccgtgtgct cgaccgcttg tcgttggcga ttcgcggcgt cgcgctcttt tgcctgatgg
     8041 ccggcgcgct ggtgatggcg gcggccctcg cggcgacgcg ctatcggcgg ctgtatgagg
     8101 cggtgatcct caaggcgctt ggcgcaaccc gcgggttgat tgcccggtca tttgcagctg
     8161 aatatgcgtt gctcggatgt gtggcgggaa tgatcggcgt cgccctcgcc agtctgttct
     8221 catgggcgat cttacgatat atcctggaac tgccgtgggc gttggagctg cccgtgctgg
     8281 gcatcggatt gggctgtacc gttctgctga cgctcctggt cggattcctc agtacctacc
     8341 gcattctagg ccagcccccc ctgaccgtgc ttcggcacga atgatcatgg tgcgatagaa
     8401 gctccgattg aatcaggtcg acctacgcgc taacacggcg cgaatcgctt cgcgctcacg
     8461 ctcgcgaaat tgcacggcga tatgaagttg ttccagattc tgttccagcg tcttgccgcc
     8521 gagatcgatt ttgcgggacg taaagaagtg ccggacgtcc tgttccagtt caggggttga
     8581 gagaccggtg aggcccccgc acattctccg caaaccgctc ttcggaaaca gcttgtccat
     8641 tcgctcccaa ttgctcttca caaatttcca ggccttctcg cggatataca cgttctgaag
     8701 caggctgctg acgaggaatg gcgcatcttg cgtacggatc tcgtcggtca gggtttttgt
     8761 taacgtccgt tccagcagct ccggtcgacg gaacgcggcc agagaaaaga gatagcgacg
     8821 ttcctcttgt ggagtggtgg cttggcggaa ccggcctgaa aattcttcgt agcgagcctc
     8881 atccccggtg aaggccagaa tcgaaacgag tgctggaatc acattgggat cgatgggccg
     8941 ggacttctgc tgaatcgcgg tgtaggcctc ccgcgcttga gcctgaatgg tttcgtcacg
     9001 tcccaaggtg cctaacgcgc gaatgatatc accgcgcaat tccttgacga gatcgcgctc
     9061 atcggcatgg ggcgaccagc cgagctgctg aaacaggggc gcaacccgat cgcgtaccaa
     9121 cgctgcgagc aagggacgat cctcttcttc caggagatgg ttgatcgtgg aaaatgaacc
     9181 cagcatgaca gcccagacat gcggatcctg ttcactgcgg aaatgttcag tcaaggcgag
     9241 gtagtcggcg gaggagacca tgccggcgac ggtggcggcc caggtatcat tcaacaggtt
     9301 gaatcgttcc accgcagtca gggtgttcag accatccttc tgcaaggcac tgagcaagtc
     9361 ggcgctgtat cgcacgcggt aaaatccatg ccccccttca ttggccagga tcgacgtcca
     9421 gccctctgga agaggaatgc gattctctcg ctcactgagc aatacgcgtc tggtttctgt
     9481 gccgcgcgcg gtgttgatcc gcagttgaac cggcacctgc caacgtggcg acgcagtgcc
     9541 ggagggtgcc gtggaggaat cgtccgcgta catgaaccgg cgttgcgtga gcatcaacgt
     9601 ggagcgtgac tcgacggaga gggacaacaa gggatacccg ggcgagaaaa tccattcgtt
     9661 catgagcgcc gggacatcct gtttcgaggc gtggccaagg gagacccaga ggtcggtggt
     9721 ctcggcattc ccataggcat gtgcatgaag gtagtggcgt acgccgtccc ggaacactgt
     9781 cggaccgata tgttgttcca gcatgcgaag gaccgaggcg cccttttcat acgtcaacac
     9841 gtcgaacatg gcttcggctt ccttcggcgc gcgcacggga aattcaatcg gcctcgtgct
     9901 cagcaggcca tccaccgata atgcggcggc gcgtgccatg ccgaaggctg tccaccgctc
     9961 ccactccggt ttccaggcat cgactaccag catttccata aacgtggcga aggcttcgtt
    10021 cagccagagc ccgttccacc aggccatggt gaccaagtca ccgaaccaca tgtgggcgtt
    10081 ttcatgggcc acgacgtccg caatgcgccc ttgctccgca tgtgtggcgg tgcgctgatc
    10141 caataacaag gcggtttcac ggaaggtgat ggcacccaga ttttccatcg cgccggaagc
    10201 gaaatccgga atggcgatca gatcgagttt gtcacctggg tagggaatat cgtagtactc
    10261 ggctagaaag ttgagtgagt agacggcgat ctcgtgcccg aatggcgtca agtgctgttt
    10321 gcctggaacg gaccatagtc ggaccggggt ctgtttggcc atgatcggcg cggtcgacac
    10381 cagttcgccc acgatgaatg ccaccaggta ggtggacatc ttcatggatt cggcgaatcg
    10441 aacgatgcgt ttgccgttct ccagccgttc gtccacgatt ctggtattcg agatggccgt
    10501 caacgccgga tcgatggcta aggttacggc gaacacggcc ttgaagtgtg gctcgtccca
    10561 acaggggaag gcccgccgcg cgtccgtcgc ttcaaactgc gtggcggcga ggctatgtgc
    10621 gatacctcga tcgtctttgt agctgctgcg atagaaaccc cggagcttgt cgttcaacgt
    10681 tccgcgaaat gcgaggctca atttccacac acccggtgga atgaccgctg ggaaggtgat
    10741 gtggcatcgc tgatgttcat catccatccg gacggtcccg gtgcgtggtg gctgtccctc
    10801 gccggacaat gtcgctgccg acacctcgag ctccgtcgca tttaagagaa tgtcagtcgt
    10861 gggttcagtg accgtcagcg tgacgatctc atgccccgtg aacgaatgtg aggggagatc
    10921 cggctccatc cgcaggtcat agtatgtcgg aatgacatgg cgcgggagac ggtagggatc
    10981 agcaattgcg gatgaagtcg tcatagtgtt gtgaggatgt tgttggttgt gcgtcgacgt
    11041 tgtttggctc cgggcctcag gcacgaagac cagggatgcg attcccggcc atgcgcccag
    11101 ccacagggcc gcatgggtag cggcacgtac ggccagagca gccgcctgtc ggcgggttac
    11161 cggcaggtcg ccctgcggcc tggtcatggc ttcggagcat gggtgacgtc ggtgccttgt
    11221 ccatgaccga cgatcaggat gctggttcgt ccgatgaaca ggccggtttc aacgatgccc
    11281 ggaatctgat tgagttcaac ttccagcgca gcagggtcat cgatgatggg catgtgcaga
    11341 tcgaggatga catgtcctgc ctcagtctga aacacgtctc cgttacgttg gcgcaagacc
    11401 gctctgccgc ccgagaccgc ttcgatgtgt cgggcggtgc tgccccaccc aaacggcacc
    11461 acttcaatgg gaagagggaa actgccgccg agcgccggca ccagtttcgt gtggtcaacc
    11521 atgacgatga atcgcttcgc cgcggcggcg acaatttttt ccttcagcag cgcgccccct
    11581 ccgcccttca cgaggttcaa tgcagggtcg acttggtcgg ctccgtcgat cgcgacatcg
    11641 atcatccagg cattgtcgga ttcgataagc ggaatgccgg attgcttcgc gagggctgcg
    11701 gtgtcgtgcg atgtcgggac ggcttgaata ttcagccctg cacgtacgcg ctcgcccaga
    11761 gcgataatga gatgtttcga cgtggtgccg gttccgagcc cgacgaccat gccatcgtgg
    11821 acaaattccg ttgccttgag cgcggcttgg cgcttttccg aatccaggtc acaggatgtt
    11881 gttgtcataa tgaaggagga agtgtgagtt gtgccgccac ggaggcagcg ccgagcaggg
    11941 cggttttatc gttcatcacg actttcaccg gtatttggtt catcagtcgt ttgtagcggc
    12001 ctttgttggt gaagccgcgc atgaatgagc cgtcctgcag tttcttcagg agtttgggag
    12061 caatgccgcc ggcgacatag actccgtcca gcgtgagggc tttcagcgcc aggttgccag
    12121 cctcggcccc gtagacggat gcgaataatt ccagcgcttg tgtcgcgatt tctgcttggc
    12181 ctttgagtcc ggcttcggcg atctcggctg ccggattgcc gaccttgatc ttctcggcca
    12241 gccacgttgg ctcattcttc ttcgtgtcgc ggagatactc gtaaatcgca tggaggccgg
    12301 ggcctgacac aatacgttcg tagctgacgt ggagatagct gccgcgaaga tgccgaagca
    12361 attcgatctc gctgtcgcta ttgggtgcaa agtccgtatg tccgccttcc gatggcatag
    12421 ggcgatagcg ggttccgtcc caatagagga tgcattcacc gagcccagtt ccagccgcga
    12481 tgagggcgag cgcctgtttt ttcttgggcg gcgcccccgc attcagcacg accatttcat
    12541 cggtcgtcag atgcagaagt ccatgcgcgg tggcttcgag atcattcaag aggcgcacgt
    12601 gggggatgac gaatcgttcg cctaaggcgg ctccatcaat gaaccagggc aaattggttg
    12661 tgcggcaacg attgtcgatg accggtcccg ccacgccaaa acaggctgcc gtgagcgtga
    12721 tgggttctgg aacaactggg gcggtctcag cggcgacatc actgccaagg tcgacggatt
    12781 cctccgtaag agcgttctcg agcgccggtt tgacgagcgg ggtgctgagg aactcgtcga
    12841 tgatctcttc aagggattta taatccgcgc tgtgaaaact gtcctcgcgc accggttcga
    12901 cccgcccgct ggtccactca tagagggcca agttcgtctt tgtcccgccg atatcacctg
    12961 ccagaatcat ggttcccctt atgatgacaa ctcatctgtc gcctcagtca atgccgatgc
    13021 ggcggcgcgg tcaaaatacc ataacagtcg ccctgtctcg ggacggatcc ttgccgccgg
    13081 ataggcatcg ggggcgtcgg cacgactttc gaaaatacgc cggacaacgg tggccttatt
    13141 cagtccggtc acgaggaaca gtaccacact cgcatgattg atcacaccta gggtgagggt
    13201 gatgcgggat cgcgtgcctt gcggggctag gccgggaacg acccagcgag ttggttcgct
    13261 gagtgccggc gtacctggaa agagcgaggc ggtgtggcca tcctccccca tgcctaagag
    13321 gaccaggtcc agtacaggcc attgttctga cgttgtggcc gtcaggcccc gcaacaggtt
    13381 ttcatagtga atggccgcgg tttcaggcga gtcctctccg cgcatccgat ggatgtgcgt
    13441 ggccgaaata ttcagtggtg tgaacaagag agtattagcc aaggcaaagt tactatcctg
    13501 atgtgtaggc ggaactgcgc gttcgtcccc gaacaggaag tgcaccttgt tccagtcgag
    13561 ctgtgtggcg aattctttac tcgccaggac catatagagt gccttaggcg tggaaccccc
    13621 ggatagagcc accagaaagc gtgcacgctt gcttatggcc tgttggccaa cagtgacgaa
    13681 gagtgttgct gcggcacgaa cgacctcttg cgcatcagca aagatgtacc gttcgggagg
    13741 gtgtgccatc tcaacggcgg gatcgacgag ttgacgtggt tcgcttcttt ggcacggatt
    13801 tccggcgcgt ggccttggcg ggtgtaagga aagtgacgag gctgcggatg gtccgtacgg
    13861 gattggggcc gagagcgatg cggacggccg ggcgctggtg tgtgtgtaac gattgcagat
    13921 caccgagggc ctgcgcttga gccaaggtgc cgaacgtata gaatttctcc gggaccgcca
    13981 gatccggctt catcgtatcg accagctcga gaaacagccc actgttcggt ccacccttgt
    14041 ggagttgccc cgtcgaatgc agatagcggg gcccgtatcc tgtcgtcgtg gccacgtgat
    14101 atcggaccat gagagctttg cgtaaggctc gcagtgccgc ttccacctgg gtggtgggag
    14161 tggtgtaagc cagaatggcc aggtagcgat tcgagcccat ctgttcgagc aattgagtca
    14221 acgcttgttt gggtggcagc acggcaatgg agggcagttt cccctgagtt tcgacctctt
    14281 tcagcacgcg tcccgtattg tctttgcttt cctgtacatt gggctggtcg aatggatgaa
    14341 tcccaagtgc atgtcccgca atggccgtcg cgaactccca ccggaagaat tcaccaccca
    14401 gatcgtaacg atccttgagc ggtatggaat acacgggctg tcccgctcgc tgcagggcgg
    14461 aaaccgttct atcaagggag gcattgcgat cgcccctgag gcgaagcgag ataaaaattc
    14521 tatctgctcc ataagcagcg ggagatgcga atggctctcc cgcgacggga atgagcccgg
    14581 tcccttcttt cccggtgctt tcggcgagca actgttctac ccagagtcca aaagaagtta
    14641 atgcgggcga ggtgagaagg gtgaccttgt cccggccgct tttggccatg gccgccatca
    14701 ggcctcccag ggcggatccc ggattttctt tggccaccgt atgtcgacag gactcccgca
    14761 tggcgagagc tcgagtgagg agctttccca cgtccaaccc aagcagggct gccggcacca
    14821 atccaaaata cgagagcact gagtagcgtc cgcctatgtc cggttggtta gtgaacgtcc
    14881 gccaaaatcc acgctcccgt gcgagttctt cgaggccggt tgccggatcc gtaatggcga
    14941 caaactgggc tccgccgcga tggccttttg tccggtggac gagttcccag aagtgagcga
    15001 agaggctcat gacttcgatc gttccgccgg atttgctcgc gacaaggaac aaggttcgtg
    15061 ccggttggat cgcatcggtg acctgtcgca cccagccggg aacggtggaa tccaagaccc
    15121 agagacgagg agcgcccttc gacgatccaa aggtgcagcg cagcacttcc gggcccaaac
    15181 tgctgccccc cattccaagt agcaccacgt ctgtcgtgcg agcctctcga gcggcttggg
    15241 cgaagcttcg caagtcggaa agtccgtctt gcatgtggtc gagtacggtc agccacccca
    15301 gcctgttatc gatttccgtc ggattcggct tccaaagggt atggtctcgc atcaaaatcc
    15361 gttcgatcgc gcgactgctt gccaattcat cgaatgttgt cgcgagggtc tgggtatcca
    15421 gaagggaggt cactttgggg cggatcggca taacacgctc tcttccatag gtggaggaac
    15481 gaagatgctg gtcggctgag ccctatctta ggttcgagga tgtgaaaagg caaaccagga
    15541 agggaaggcg atacgcgatg gcggagtcag ttggagcgag agagatgaat gatggaggtg
    15601 gagaagcgac cgaaggctaa tgcgtagcgg gcccgacgct atgtttcttc ctcctgatcg
    15661 cccaagtcag ccaggactag cgaaatggaa ttttgcgatt ccagtgcgcc gacgatattg
    15721 acgggtgtgc ccacggcgac ctggtcaaac agttgggcaa tctgcgggtt atccagcacg
    15781 atactgccgt acggttgatc cgaaattcct tgcgcgatgc catgaagctg aatgaggcct
    15841 ggcatatgtt ggcttttggg gatgagtcct gctttctggg cttcctcgaa tcggcgccgg
    15901 tcgtctatat tgggatagtc gagaatcagg gcgcggaaat agggcgtttg tccaggtccg
    15961 cgtttacgtt gaatgcgata ttcgccttcg ggagtagcgc catcaccgta gtgctgtttt
    16021 gcccggattc ctcttgatcc taatcgaata ggataggtca gaactttgcg accgttctta
    16081 tatagggtga gggagcggtc ggccttgctg acgacaatgg cctgggcccg atgagttcga
    16141 gaccagtcaa tggtgcgttg tgccgattcc cgccacgccg cgatcagatc atcgtccgca
    16201 taccggccta actcattggt gagcagggcg gtctgagtca ggaggattct accggctcgt
    16261 tccgaagcct ggatcgaatg ttcaaattgt ccctgttcga ggaacaggcg agcctgtttg
    16321 acgtgcactt cagtttcgac cggatgctcg cccagcacca cgcggctgcc gatgtctccc
    16381 actcgagagt tcaggaggcg aagccgctgc tcgattttgg cgagtttcgt ttcggcagtc
    16441 gtgcgttgag cctgaatgcg gctattgacg tcgctcagga gctgcgtgcc ctcgacttga
    16501 agggcttcca gatcggtttc cagctcattg tcttcccatg gccatcgaac aatgttctcc
    16561 tcggatcgga ttcggccttg tagcgcaatc cattggcggg aaaacttgga ataggctgcg
    16621 gggttcacgt cggaggctcg cagtgttgac aggtcacggt cgagactctc aatggattcc
    16681 accaactcct caggaacggt ttcgacgcat ccgcccaagc tcatcgataa cagcgcgacg
    16741 agggtaacgc gagtggcgat gcggcagatg taacgtgagc ggctcatcat tcttgcattc
    16801 tatcgtgcgg atggctgtcc cgcgagttct
//
LOCUS       DAOQEW010000072        16825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_905_length_16825_cov_6.129338, whole genome shotgun sequence.
ACCESSION   DAOQEW010000072 DAOQEW010000000
VERSION     DAOQEW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_16825_cov_6.129338"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLS35_09515"
     CDS             complement(<1..257)
                     /locus_tag="PLS35_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18943.1"
                     /translation="MNTLHDIEQFWDANPCGETLVGKDQEWATFFNRYDEFRYRTEGH
                     ILGELDRLQLAGKNVLEIGIGQAADSAQIIRRGGRWHGLDLT"
     gene            complement(257..1432)
                     /locus_tag="PLS35_09520"
     CDS             complement(257..1432)
                     /locus_tag="PLS35_09520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI18944.1"
                     /translation="MTTRNDTIEVIGPTPPPYHGVSVATQVLLDALPHSQLAVVHLDT
                     ADRRGIAHVDQPDLWDVFLFIRQWFAHLFILLRKRPRICYLPLSQSRVGFLRDSLFMV
                     PAFLTGSAVVAHLHGANFYQVYEGGGTAFKWYVDQVLKRVAKFVVLGDMLKPIFHRWA
                     SLESIAVVPNGVPELSTDGSPGPHCVRSAGPRIVFLSTLSRNKGLFVLLDAVPLVVQE
                     FPTAEFHIAGPWWGADTERLVRAQLEQGNVSSQVIFHGQLAGAKKMAFLRSGDLFVFP
                     GIQQEGQPLTVLEAMCAGLPVIATDRGCLRETVMEGETGFVVPPNSPTAIAERILLLL
                     REPHIRQRLGANARLRYEREYTMPLYAARMADLFAQVSQSDYHENPKSMDSRHWSIR"
     gene            complement(1429..2607)
                     /locus_tag="PLS35_09525"
     CDS             complement(1429..2607)
                     /locus_tag="PLS35_09525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI18945.1"
                     /translation="MKLAVLTNILTPYRIPLFQAMAEQVDEFSIILMAQQEENRLWKI
                     EDVPFPVYVLPGFHVRPWGAPVAVHLNYGVTARLTQLDPDVVLNAGFAVANIAAYWYC
                     RRAHIPFIHWAHLSLNDGAQASFVRRRIRHWLIGRSAGAVGESSHARDAFIHYGAPKE
                     RILVATMPLDVTGLHDRVLARKQWPETSTPLNTLSKPVLLSIGQLIPRKGYHELFAIY
                     ERLLTTHPHTSLLIVGDGPDRQHFETVVREKGWTRVHFAGFVQAQDLPRYFALSDAFI
                     FHTLYDPFGLVLSEAMAAEVPVVSSIHAMATHDLVEDGVTGFRIDPLQPEASAETVRH
                     LFDMSSDQRTSLIDAAYRRVLPCDSRTSASRIVEFLRSFRTPHEPRMATTPSPSQRIS
                     "
     gene            complement(2626..4152)
                     /locus_tag="PLS35_09530"
     CDS             complement(2626..4152)
                     /locus_tag="PLS35_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNI18946.1"
                     /translation="MKKSDEHSHQGQTDQLVVAGSAVLLSSIFGNGLNYLFGVFLARF
                     LGADQFGLYALALAFFTTLTLVVPLGMDAGAIKFISEYRTRQDSEGAQRIVLMAGTVT
                     FLTAALTAICLAGLSSLISLSVYHKPELNILLLYVAAGIPLYALTSILLASLQSHHTV
                     RPLIVVRYLWEPIGKFILAGLVISAGWGLAGVFGALLIAMLVSLTLTLGFLRTVVRFR
                     RPMIDLWSREEAARLMTYCLPLGVATVFGVVAPRADILMLGSWVNVHEIGIYQAAFQT
                     AAALALILGALESSLTPFFGQLHAQNDIAGLRHMYQTASKLVLLFTVPLFVLLAVFSE
                     EVLSLFGTEFRAGGTLLIVLASGQLLSSAGGSPNNLLLMGGHSRLVMWNTIGVGLLAF
                     GVFACTIPLWGVLGAAIGAASTQVFGVGLRILQVWRIFHIQPFSQRLVKPLLAGLGSA
                     VLGLSLKPDAPALLLPGLGLMMGLVYIVLLIALKLDPQDRQALNALVSKAKMIVAVRA
                     "
     gene            complement(4298..5632)
                     /locus_tag="PLS35_09535"
     CDS             complement(4298..5632)
                     /locus_tag="PLS35_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI18947.1"
                     /translation="MAYAFGFLILALLVKDTAPIGDQYVLRTLSQGLCLTAGFWWLFT
                     QGNIIAMRRYWLILLYSAVLCSTAFVSMDSTRVLLQVLSLVAVTLFFMSYCEQAQQMP
                     SMHDTAARIVLYALLPVCLGSLLLYKIAPTLAFDQTVERMVWDNPHRFKGLFGKPAGI
                     AAASGILLGLCAFTKVHWLIRAVGALSSLMCLYLTLSRSFWVGSSVALLVTAFLYAKR
                     KGLLLGAGIGGLLLILATVSIMDTKIPGLNQSRSMRSDSLENLSGRTTLWTLALDRFW
                     DRPVLGYGYTVGHQAFLYSRKAKGADVAGDNVDLFRNETFSLHNGYVQALLDSGGMGA
                     LLYTIILAITVWRIVWYDRVKAHAAALYSLVFLSIANIGETLVYTPATFHAAFYWYVA
                     VLALGLRFTVEPPPASFAEPIVLPVRERTTKYPIVSRPEPQVQQGKTSPGLC"
     gene            complement(5753..6916)
                     /locus_tag="PLS35_09540"
     CDS             complement(5753..6916)
                     /locus_tag="PLS35_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HNI18948.1"
                     /translation="MLSRRQFLQISAACTASALCASLPRFAQAQETFESRVIFDESLS
                     WVTEHSAHSMCQRMKQAGFNVVMPCVWHGRGTSWPSNLAPWDSYKLDEMARWNSSFDP
                     LGKLVEIAAQYQIEVHPWFNVTLRQREFLPHFYGPGTPADAFDVHRPDFRTFISNLMI
                     ECVSRYPVHGINLDYIRSVSICQSPECARQYHHHTNRNLMLDRLSYGASADAREAIAD
                     WNEQAVGDIVRRVSSHLRKNQPNVLISLCGHPGHPNLYIQGQKSIKWADEGLIDVVYD
                     MRYPAEPNWEEIRSVQKTMKRPEAYAVLIGNYDLAGAHQISVSSSGARVAAQIVRSRQ
                     VAPGNGVGVYLYSLLNDEQITALRSGPFQTPASPKWSRAKGLDLSTPTGLLVQ"
     gene            complement(6920..8131)
                     /locus_tag="PLS35_09545"
     CDS             complement(6920..8131)
                     /locus_tag="PLS35_09545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI18949.1"
                     /translation="MSHVKSIALIQLHIPHYRVPLFAALQAEAARRGYALTVYSGEQQ
                     PMPFSHRKLSLRYLSHDKSGGCWMEGLAEAVAGSDIIIAPHGLQCLTIPFLWLQRNRL
                     CTSWIWWGHGYNFQASTQGSTAEWITSAIKNFLLTRGDGVITYTHGGAQYWCNRGLPP
                     ERVAPFLNTIDVEGLREARRQVTDDQLSLVRERLALGKKQVLLFSGRLYAEKQVDFLL
                     EAFSKIQQACPETALLILGEGQERARLEQLCSSLHLHDVHFLGEITDPHETSIYFKLA
                     ELLVIPGLVGLAIVHGFAFNLPLMTTERPFHSPEIEYLSSLNGRMTRHDTDAYAAEII
                     RVLSSPQEIEAMRWHCAESAQQLTLAASSSRFVQAVAQFSNPTGSLTSNRRRSQRTSF
                     SSSAATPSLRR"
     gene            complement(8625..9725)
                     /gene="wecB"
                     /locus_tag="PLS35_09550"
     CDS             complement(8625..9725)
                     /gene="wecB"
                     /locus_tag="PLS35_09550"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNI18950.1"
                     /translation="MTRIDLIAGARPNFMKIAPIIDALNAARSRGSHLRFRLIHTGQH
                     YDRAMSGSFFEELGIPDPDINLEVGSGTQAEQTSAIMVNYEKVLAKDKSHLCLVVGDV
                     TSTMACSIVARKMGIPVAHVEGGIRSNDWTMPEEINRVVTDSITNWFFTTSETANENL
                     RRAGVTSDRIFFVGNTMIDTLLKHADRLRPPACWSSLALKPNHYFVVTLHRPANVDGE
                     QQLLGLLRAIAEGTQGLPVVFPVHPRTAKNLRESGQALPSMHYVDPLGYLEFNYLVKH
                     ARGVITDSGGITEETTVLGVPCLTLRDNTERPETVTIGTNELIGTDPAKLPPALQRLM
                     AGQWKKGAIPPLWDGKTAVRIVEHLTTLLAKP"
     gene            complement(9920..10159)
                     /locus_tag="PLS35_09555"
     CDS             complement(9920..10159)
                     /locus_tag="PLS35_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18951.1"
                     /translation="MTSLTRPVFLLMLCAFSAGCSTAEWVHPNRPKDQFTLDYNKCQA
                     DTMRDPKLQQGIQLLIIQATERCVQKQGWRLVEKE"
     gene            complement(10266..11165)
                     /locus_tag="PLS35_09560"
     CDS             complement(10266..11165)
                     /locus_tag="PLS35_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI18952.1"
                     /translation="MIGHAASPTMLVVDASPETWAMALEEGKLRGISVITAPDPQVAL
                     AMIDMAAPDILMTDLFPADQAGVLLIRELRMRSPKTAILATGESGDAETILNVVRAGA
                     GDYLRKPLLVEELGLALDRALRQLPSTVENIAGVEQVDYRLVLGTNPDHVEACVTWLI
                     QQTALTLPEMQRLHLRTTLIELIVNAVEHGSLEILYQEKHAALSSDQFERLIEERRRH
                     PRFARRRVVVQASHDKTRRRLRYAITDEGKGFAWSRFVATPEQPCDSRHPNGRGVFLA
                     KAFFPDLTYNERGTEVTFSVPVP"
     gene            complement(11162..11521)
                     /locus_tag="PLS35_09565"
     CDS             complement(11162..11521)
                     /locus_tag="PLS35_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNI18953.1"
                     /translation="MDSQPVMDLAAALDRFDGDQDLFLTLAGLFVERSGQALAALQTA
                     LSAQDLPVLVKEAHKLKGSAMEFCARPAVAAAAHLEEAARKAAAQDIPALAEQTRAEV
                     ERLTAALAEIMQKGFPS"
     gene            complement(11521..>12513)
                     /locus_tag="PLS35_09570"
     CDS             complement(11521..>12513)
                     /locus_tag="PLS35_09570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNI18954.1"
                     /translation="ELKSSQVVLMTAHDRPEQIAEGLSRGADDFLSKAASKQEVLARV
                     QASLRSSALVREIEHTRDDLDRSHRLLSAKQAELESELQSAADFVRAQLPLPGMPATG
                     VSMQWAYQPSLALGGDLFQVCSWGADALGLYILDASGHGVAAALRAVGLMSFLREDNL
                     AKVVGSFDPGAIITEANRLFPLTQDGEYFTLWVGRLDISSRMLSYATAGHSGAFLQAA
                     GDGSRWLSMASLPLGFDPHSVFESMHTPLLSLDRLYLLSDGIYEAPSPTGELWGRDRL
                     QATLEAHRSHSLGQSIADTMATARQWLAGEIFPDDVALLGLEMLDTLLGQKERA"
     assembly_gap    12514..12613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12663..13013)
                     /locus_tag="PLS35_09575"
     CDS             complement(12663..13013)
                     /locus_tag="PLS35_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNI18955.1"
                     /translation="MQITERRVGESVILDLVGELTYANRATFKASVERSRAGGVRHLI
                     LNMQGVRFLDSSALGTLALLTQSLSATRGTIGLLNPQSYVKEILTLANLHQLLPVYHS
                     EQDALAACRLPKAG"
     gene            complement(13046..13384)
                     /locus_tag="PLS35_09580"
     CDS             complement(13046..13384)
                     /locus_tag="PLS35_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNI18956.1"
                     /translation="MKITSEIHNHKVTLKLEGNFTYTQRKPFQEMLKSVSVDPMEHIV
                     IDLSQVAFLDSAALGLLMISHRQLQAEKRTLSLAYPQATVRQIIELANLHKTIPLIES
                     PASTVTKKSA"
     gene            complement(13416..14462)
                     /locus_tag="PLS35_09585"
     CDS             complement(13416..14462)
                     /locus_tag="PLS35_09585"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNI18957.1"
                     /translation="MAKIRVLTIDDSALMRQVLAELLSKDPGIEVIGSAPDPYVAREK
                     IKALNPDVLTLDVEMPKMDGLTFLEKLMRGRPTPVIMVSSLTEAGCQTTLRALELGAV
                     DFITKPKIDLREGMDQVAQDLIAKIKAAATASVRPTTAAASSTSRPTSLNSAMIKTTD
                     MIIAIGSSTGGTEAVKDVLQVLPPNTPPILITQHMPERFTKTWADRMNQLCRISVKEA
                     EDGDSVLPGHALVAPGNYHMTLVRSGARYSVRINQDEPVNRHRPSVDVMFDSVAQYAG
                     GNAVGVILTGMGGDGAKGLLRMKEAGAFTIAQDEASCVVFGMPKEAIKLGAADIVRPL
                     GDIASAILTQVTRA"
     gene            complement(14524..15141)
                     /locus_tag="PLS35_09590"
     CDS             complement(14524..15141)
                     /locus_tag="PLS35_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18958.1"
                     /translation="MAVIDTDAFSHIRRMRDGRFPHEVACILPGEFFVSREPMVVYTV
                     LGSCISACIRDPIVGVGGMNHFMLPAPKEHQSGDAWGGESTRYGSFAMEQLINEILKR
                     GGLRHRLEVKLFGAGKIYDGNIDVGARNTEWVLQYLKTEGYALAKSDLGDVYPRKVYY
                     FTDSGRVLMKKIERVKNRTIYERETEYQHRIEVEPTVQQDNITLF"
     gene            complement(15147..15965)
                     /locus_tag="PLS35_09595"
     CDS             complement(15147..15965)
                     /locus_tag="PLS35_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HNI18959.1"
                     /translation="MDYPISKKEYDQIRTLLYDESGISLGESKQSLVVSRLTKRLRDL
                     QLGDFSSYYEYVTEDNSGEEFTRMLDLLSTNKTDFFREPKHFDFLRERILPSLEKEKR
                     IRIWSSACSTGEEPYTIAMTLHEGVQQPAQWNFQILASDISTRVLAHAAKGLYAEDRV
                     REVPPEIVKHHFLQGRGDSAGLVKVKPHLSAIIKFRRLNLMDDHFPIKAPLDVIFCRN
                     VMIYFDRPTQERLVNKFYHHLKPGGHLFIGHSESLQWVSHPFKTIAPTIYWKES"
     gene            complement(15970..16431)
                     /locus_tag="PLS35_09600"
     CDS             complement(15970..16431)
                     /locus_tag="PLS35_09600"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNI18960.1"
                     /translation="MDVKTLLWYLVLSSLTVAYLVVLAGVKAAKSHDVTHHSHRMIVG
                     CTIVGIWLVAYVLKQALFGRESFQGPESAYWSVYVPIFVTHMLLAVTTIGLGAYNLYM
                     GLHRLRFGSVGAMVAGMTTHRRMGNALIWTFSGTMVTAYLVYLMLFVWYRS"
     gene            complement(16636..>16825)
                     /locus_tag="PLS35_09605"
     CDS             complement(16636..>16825)
                     /locus_tag="PLS35_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18961.1"
                     /translation="NQAPARVASSVKSAVKPVASAPKLGLKKPGVPGKPAEAKQPAGV
                     AAGNGHDRRKKDDDFEEF"
BASE COUNT         3760 a         4669 c         4797 g         3499 t
ORIGIN      
        1 gtcaggtcaa gaccatgcca gcgtccgcct cgccgaatga tctgtgcact atctgcagcc
       61 tgaccgatcc cgatttcaag cacgtttttc ccagcgagct gcaggcggtc gagctccccg
      121 agaatgtggc cttcagtccg ataacgaaac tcgtcatagc gattgaagaa cgtcgcccat
      181 tcctgatcct tgcccactaa ggtttcgccg caaggattgg catcccaaaa ctgctcgatg
      241 tcgtgaagcg tattcatcag cgtatgctcc agtgtcgcga atccatcgat ttcggattct
      301 catggtagtc cgattggctg acctgtgcaa atagatccgc cattcgtgcg gcatagagag
      361 gcatcgtata ttcacgttca tatcgcaatc gagcattggc accaagcctt tgccggatgt
      421 gcggctcacg gagcagcaga agaattcgct ccgctatcgc ggtcggtgag ttcggcggta
      481 ccacaaaccc ggtttcacct tccatcacgg tttcacggag gcatcctctg tccgtggcaa
      541 tgactggtag tcctgcgcac atggcttcca acaccgtcaa cggttgccct tcctgttgaa
      601 tgccggggaa gacgaacaga tctccactcc gcagaaacgc catcttcttc gcgccggcca
      661 gctggccgtg gaaaattacc tgcgagctca cattcccctg ctccaattgc gctctcacca
      721 gacgttcagt atcggccccc caccaggggc cggcgatatg aaattcggct gtgggaaact
      781 cctgcaccac cagcggcacg gcgtccagca ggacgaagag acctttgttc cggctgaggg
      841 ttgagagaaa gacgattcgg ggtcctgccg accggacgca gtgtgggcca gggctcccat
      901 cggtcgatag ttccggcacc ccgttcggca cgacggcaat cgattcgaga gaggcccacc
      961 tgtgaaagat cggtttgagc atgtcgccaa gcacgacaaa tttggccacg cgtttcagca
     1021 cttgatcgac gtaccattta aacgctgttc cgccgccctc atagacctga taaaagttcg
     1081 ccccatgcag atgtgccacg acggctgagc ccgtaagaaa cgctggaacc ataaacaggc
     1141 tgtcacgcaa aaagccaacg cggctttggg aaagcggaag gtaacaaata cgaggccgtt
     1201 tccggagcag aatgaagaga tgggcgaacc actggcgaat gaagaggaag acgtcccaaa
     1261 gatccggttg atccacatgg gcgatgccgc gcctgtccgc cgtatccaga tggacgacgg
     1321 ccagctggga atgaggcaac gcgtcgagta acacctgtgt cgccacactc acaccatgat
     1381 aaggcggcgg agtcgggccg atgacttcga tggtgtcgtt gcgagtcgtc atgaaatacg
     1441 ctgtgagggg ctcggtgtgg ttgccatacg gggttcgtgc ggcgtcctga aactcctaag
     1501 aaactccaca attcttgatg cactcgttcg actgtcacag ggaagcaccc ggcgataggc
     1561 tgcgtcgatc aaactggtac gttggtctga agacatgtcg aaaagatgcc ggaccgtctc
     1621 tgctgaagcc tctggctgca agggatcaat acgaaaaccc gtgaccccgt cctcgactaa
     1681 atcatgagtg gccatggcat gaatcgatga gacgacagga acttcagccg ccatggcttc
     1741 actcagcacc aggccaaaag gatcgtaaag tgtatggaaa atgaatgcat cgctgagggc
     1801 aaagtagcgt gggagatcct gcgcctgcac aaaccccgca aagtgtactc gcgtccatcc
     1861 cttttcccgc accactgttt cgaaatgctg tcggtccggt ccatctccaa cgatcagcag
     1921 ggatgtgtgc ggatgtgtcg tgaggagtct ctcatagatg gcgaagagct catggtagcc
     1981 tttacgcgga atgagttggc cgattgagag caggactggt tttgaaagcg tgttgagcgg
     2041 cgtagaggtt tccggccatt gtttgcgcgc aaggacacga tcgtgaagac ccgtcacatc
     2101 cagaggcatg gtcgccacta atattcgttc tttcggcgcg ccgtagtgaa tgaacgcgtc
     2161 tcgagcgtgg gaggattcgc cgactgcgcc agcgctgcgt ccgattagcc agtgccggat
     2221 tcgacgccga acaaacgagg cctgtgctcc atcgttgagg gacagatgag cccaatggat
     2281 aaatgggatg tgtgcgcgtc ggcagtacca atacgccgct atgttggcta cggcaaaccc
     2341 ggcattcagg acgacatccg gatcaagctg cgtcagccgg gcggtgacgc cgtaattcaa
     2401 gtgcacggcc acgggcgcac cccaagggcg cacgtgaaat cccggtaaca cgtaaaccgg
     2461 aaaaggcaca tcctcaattt tccacagccg gttctcctcc tgctgcgcca tcaatatgat
     2521 ggaaaactcg tccacctgtt cggccatcgc ctgaaacaat ggtatgcgat agggtgtgag
     2581 aatattcgtc aggacagcga gcttcataag tagtctgtat cagtatcacg cacgcactgc
     2641 aacgatcatt ttggctttgc tgaccaaggc attcaacgcc tggcgatcct gagggtcgag
     2701 tttgagggca atcagaagaa caatatagac cagccccatc atcaatccga ggcccgggag
     2761 caacagggcc ggcgcatcag gtttcaacga caagcccaac actgcgctgc caagacccgc
     2821 caagagtggt ttcacaagac gctggctgaa cggctgaatg tgaaatatcc gccacacctg
     2881 caggatgcga agcccaacgc caaatacctg ggtggaagcc gctccaatag ccgcgcccaa
     2941 cactccccac aggggaatgg tacaggcgaa caccccgaag gccaggaggc cgacgccgat
     3001 cgtgttccac atgacgagtc gtgagtgtcc tcccatcagg aggagattat tgggtgatcc
     3061 tcctgcgcta ctgaggagtt gtccggaagc caacacgatt aacaatgtgc cccccgccct
     3121 gaattccgtt ccaaagagag acagcacctc ctcgctgaac acagccaaga gcacaaacaa
     3181 ggggacagta aataagagga cgagttttga ggcagtctga tacatatggc gtaggcccgc
     3241 gatgtcgttc tgtgcgtgca attggccgaa gaatggggtg agtgaggatt ccaatgcgcc
     3301 aagaattaac gccaaggcgg cggccgtctg gaatgcggcc tgataaatac cgatctcgtg
     3361 cacattcacc caggaaccga gcatcagaat atcggcgcgc ggggccacga cgccgaacac
     3421 ggtcgccacc cccagcggaa ggcaatacgt catcaatcgt gcggcttcct ctcggctcca
     3481 aagatcgatc atcggtcgac gaaatcggac gaccgttcga aggaaaccaa gcgtcagagt
     3541 caggctgacc agcatggcaa tgagaagtgc gccgaacacg ccggccagcc cccatccagc
     3601 cgaaataacg agaccggcga ggataaactt gccgatcggc tcccacagat accttacgac
     3661 gatgagtggc cgaaccgtgt ggtgggattg aagagacgcc aataagatgc ttgtcagcgc
     3721 gtatagagga ataccggccg ccacgtatag cagcaaaatg ttcagctccg gcttgtgata
     3781 gaccgacagg ctgatcagtg aggacaatcc ggccagacag atggcggtca acgcggcggt
     3841 caaaaaggtg actgttccgg ccatgagcac gatgcgctgc gccccctcag aatcctgacg
     3901 tgtccggtat tcggaaataa acttgatcgc gccggcatcc attcccagcg ggaccaccag
     3961 ggtcaaggtt gtgaaaaacg cgagggctaa tgcgtacagc ccaaattgat cggctcccag
     4021 aaatcgtgcc aggaatactc cgaacagata attgagtccg tttccaaata tcgatgaaag
     4081 aaggactgcg gagccggcaa ccaccagttg atcggtctgg ccttgatgcg aatgttcgtc
     4141 agatttcttc atatcaagtc tggagtcttc acagcatggg cccgtgtccc agagacgtta
     4201 gtccgacacg ctgggtagtt cgcgcaatgt tttaccattc acgcaggctg catcgttcct
     4261 gtcggcagag cgcgaaatca tctgcgcgtt ggcaaaatca gcagaggccc ggagaagtct
     4321 tgccctgctg aacctgaggt tcaggccgtg acactatcgg atattttgtc gtccgttcac
     4381 gtacagggag aacgatgggc tcagcaaatg acgctggggg tggttcgacc gtgaagcgga
     4441 gtcccagcgc aaggactgcg acataccagt aaaacgcagc gtgaaaggtt gccggcgtat
     4501 acacgagtgt ctctccgatg ttcgcaatgg ataggaacac cagggagtac aatgccgcgg
     4561 catgggcctt tacgcggtcg taccatacga tcctccatac cgtgattgcg aggataatgg
     4621 tgtagagcag tgcgcccatt cctcctgaat caagcagcgc ctggacatat ccgttgtgca
     4681 atgaaaatgt ttcattacga aacagatcga cattgtcacc ggccacgtca gcccctttcg
     4741 ccttccggct gtagagaaac gcctggtgtc caaccgtata cccatatcca aggaccgggc
     4801 gatcccagaa gcgatcaagc gcgagggtcc agagtgtcgt tctgccggag agattctcca
     4861 acgagtcaga tcgcatggat cgtgattggt tgagtcccgg aatttttgta tccatgatgg
     4921 agaccgtcgc caggataagc agcagccctc caatccccgc tccaagcagg aggcctttcc
     4981 gtttggcata caaaaatgcc gtgaccagca acgcgacaga cgatccgacc cagaagctcc
     5041 gtgaaagtgt caggtacagg cacatgagag aggagagtgc gccgacggca cggatcagcc
     5101 aatgaacctt tgtaaacgcg cacaagccaa gcagaatgcc agacgcggct gcgattcccg
     5161 ctggtttgcc gaaaagacct ttaaagcggt gcggattgtc ccataccatg cgttccaccg
     5221 tctgatcgaa cgccaaggtc ggtgcgattt tatagagcaa caggcttccc aggcagaccg
     5281 gcagcagggc atacagaacg atacgagcgg ccgtgtcatg catggaaggc atctgttggg
     5341 cttgttcaca atagctcata aaaaacaggg ttactgccac cagcgagaga acctggagca
     5401 agactcgtgt cgagtccatg gagacaaacg ccgtgctgca caggactgcg ctgtagagca
     5461 gaatcagcca gtagcgtcgc atggcaatga tgttgccctg cgtaaagagc caccagaatc
     5521 ccgctgtcag acagagaccc tgcgagaggg tccgcaatac gtattgatcg ccgatgggtg
     5581 cggtatcttt gaccagcagc gcaagaatca aaaagccgaa ggcataggcc atgaaccgca
     5641 tcctatctga aaaggaaact gcgtagctct gaacgagcta cgcattatat acgtcacaat
     5701 gataggcgat gtaattgaaa ttcgaagagc gcgcaatccc gatgcgactg gactattgga
     5761 cgagcaggcc ggtcggcgtt gagagatcca acccttttgc gcgcgaccat ttcggggagg
     5821 ccggcgtttg gaagggaccg gaccgcaatg cagtgatctg ttcgtcgttg agtaagctat
     5881 atagatagac acctactcca ttccccggtg caacttgacg gcttcgaaca atttgagccg
     5941 ccacgcgcgc tcccgagctg gacacggaga tttgatgcgc tccggccaga tcgtagttac
     6001 caatcagaac ggcgtaggct tcgggccgct tcatggtttt ctgaacggat cggatctctt
     6061 cccaatttgg ttcggcgggg tagcgcatgt cgtagaccac atcgatcaag ccttcatcgg
     6121 cccatttaat gcttttttgg ccttggatat acaagttcgg atgcccggga tgtccgcaca
     6181 ggctgatcag aacattgggt tggtttttcc gaagatggct ggacacccgc cgcacgatat
     6241 ctccgactgc ctgttcgttc caatcagcaa tggcttcccg cgcatcggcg gaggcgccat
     6301 agctcaatcg atccaacatg aggttgcgat tcgtatgatg atgatattgc cgggcacact
     6361 ctgggctctg gcagatggaa acgctacgga tgtaatcgag attgatgccg tgaacgggat
     6421 accgcgacac acactcaatc attaaatttg agatgaacgt gcgaaagtcc ggacgatgca
     6481 cgtcgaatgc gtcggctggc gtgcccggcc catagaagtg cggcaggaat tctcgctgac
     6541 ggagtgtgac attaaaccag gggtgaacct caatctgata ctgcgcggcg atttccacca
     6601 gctttcccaa gggatcaaag ctggaattcc accgggccat ttcgtccaat ttgtaactgt
     6661 cccagggggc caggttggat ggccaactgg ttccccggcc atgccagaca catggcatca
     6721 ccacgttgaa accggcttgc ttcatccgtt gacacatact atgtgcggaa tgttcggtca
     6781 cccagctgag gctctcatca aaaatgaccc ggctttcaaa ggtttcctgg gcctgagcga
     6841 atcgcggaag tgaagcgcag agagctgacg cagtgcaagc ggctgagatc tgcagaaatt
     6901 gacgtcgtga cagcatgaac tatctcctca gggatggcgt agcagcggat gatgaaaacg
     6961 atgttctctg tgatctgcgc cggttgctcg taagcgaacc tgtcggattg gagaactggg
     7021 cgacggcttg tacgaatcga ctcgaggatg ccgccaatgt gagctgctgg gcgctctcgg
     7081 cacagtgcca tcgcatggcc tcgatctcct gcggcgacga gaggacccgg atgatttccg
     7141 ccgcataggc gtccgtgtcg tgcctggtca tcctgccgtt cagggacgac agatattcga
     7201 tttccggact gtgaaacgga cgttcggtcg tcatcagagg aaggttgaac gcaaatccat
     7261 ggacaatggc cagacccacg aggcccggta tgacaagtag ctcggccagt ttaaaataga
     7321 tactcgtttc atgcggatcg gttatttcac ccaaaaagtg cacgtcatgc aagtgcagtg
     7381 agctgcacaa ctgttcgagc cgcgcacgct cctggccttc gccaagaatc aataaagcag
     7441 tttctgggca ggcctgttga attttggaga atgcttcaag aaggaagtcg acctgctttt
     7501 ccgcatacag acgtccggaa aagagcagca cctgtttctt gccgagcgcg agacgttctc
     7561 gtaccagcga cagttggtca tcagtcacct gccggcgtgc ttccctgagc ccctcgacat
     7621 cgatggtatt cagaaatggc gccactcgtt cgggcggcag gccccggtta caccagtatt
     7681 gagctcctcc atgcgtgtag gtgatgaccc catcgcctcg tgtcagtaaa aagttcttta
     7741 tcgcggaggt gatccattca gcagtggaac cctgtgtcga cgcctgaaaa ttgtacccgt
     7801 ggccccacca gatccaggat gtgcagagac ggttccgctg cagccagaga aaaggaatcg
     7861 tcagacattg caggccatga ggcgcgatga tgatatccga gccggcgaca gcctcagcaa
     7921 gcccttccat ccagcagccc ccgctcttgt catgactcag gtatcggagg gacagtttcc
     7981 gatgcgaaaa gggcatgggc tgttgttcgc ctgaatagac tgtcagggca tagcctctgc
     8041 gggcagcctc cgcctgaagc gcggcgaaca ggggcacacg atagtgcgga atatgtagtt
     8101 gaatgagtgc aatagatttt acgtgcgaca cgcatatctc cgtcggttcc tagacccctt
     8161 gttcggcgag gggaaaatgt gacatggcct ggtccattga ccgtcgtgag gagtatgcaa
     8221 ctgacaggcc tgcgctaccc gctccgaccg ttcttctaaa accatgatga aatggggaat
     8281 taggacggaa cgatacctcc tgcgtaccgg ccgatgtcgt cagtcagccc agtgaattga
     8341 gcaggattgt acatagcggc cgtggcctga atacgtatgc ggctatgtgt tgtagaatga
     8401 caagctagga gaattctagg gacgtttgac tctggcctat cgtatgtact ctgcgagaag
     8461 ttgacgattt cggagagaac cctcgtcaac aatttggcga ggagccaagc acaatgtcag
     8521 attgtcgcca ttgaatggct actgactatt ggcaggtaga gcgtctgcgg gttggttcgg
     8581 ttttccgcac caagcaggag accatggtcg tgttgtctta gcgactaggg ttttgcgagt
     8641 aaggtcgtca gatgttctac gatccgcacg gcggtcttcc catcccagag cgggggaatt
     8701 gcgcctttct tccactgccc tgccatcaat cgctgcagcg ccggcggcag cttcgctggg
     8761 tcggtaccta tcagttcatt ggtaccgatg gtcaccgttt ccggccgttc ggtgttatcg
     8821 cgcaaggtca ggcagggaac gcctagcacg gtggtttcct cggtgatgcc gcctgaatcg
     8881 gtaattacgc cgcgggcatg cttcaccaaa taattgaact cgagataccc taacggatcg
     8941 acatagtgca tcgacggaag tgcttgccct gattcgcgga ggtttttggc agttcgagga
     9001 tgaaccggaa agaccaccgg taaaccttgg gtaccctccg caatggcccg taacagcccc
     9061 agcaactgct gctcgccgtc cacgtttgcc ggacgatgca gggtgacaac aaagtagtga
     9121 ttgggtttga gggcaagaga actccagcag gccggaggcc gtaaccggtc cgcatgtttg
     9181 agcagcgtat cgatcatagt gttgccgaca aagaagatac ggtcgcttgt gacacctgcc
     9241 cggcgcaaat tctcattggc cgtttcgctc gtggtgaaga accagttggt aatggaatcc
     9301 gtgacgacgc gattgatttc ctccggcatt gtccaatcgt ttgaacggat accaccctcc
     9361 acatgagcca ccggaatccc catcttgcgg gcgacaatcg aacaggccat ggtcgaggtc
     9421 acgtccccga cgaccaagca gagatggctt ttatctttgg ccagaacttt ttcgtagttc
     9481 accatgatgg ccgacgtttg ttcggcttgc gtgccggaac cgacttccag attgatgtcc
     9541 ggatccggga tacccagttc ctcgaaaaag ctgcccgaca tggcgcggtc gtagtgctgc
     9601 cctgtatgaa tgagtcgaaa acgaagatgg cttccacgcg accgggcggc attcagcgca
     9661 tcgatgatcg gcgcgatttt catgaaattc gggcgtgctc cggcgatgag atcgatgcgt
     9721 gtcatggcat ggtttcctgt acggcggcgg aggtggatgg tcgatcagtt cattacataa
     9781 cgaaccttga tcgatccgtc aacaaaaacg actcgaagac aagtgatgcg aaaaggtggg
     9841 aggcgtgtca cggagcgaat ggcgagggct gacacaggca cagaagccgg cttgcctgcc
     9901 gatcgccatt cgggagtggc tactcttttt ctaccagccg ccacccctgt ttttgcacac
     9961 agcgttccgt ggcctggatg atcaggagtt gaatgccctg ctgcagttta gggtcgcgca
    10021 tggtgtcggc ctggcacttg ttatagtcca gggtaaattg gtccttcggg cgattggggt
    10081 gaacccactc ggcagtactg caaccagccg aaaaggcaca tagcatgaga agaaacaccg
    10141 gacgagtgag agaagtcatg agacgcctca tttgtgagcg gtcaggaggg aacggaatca
    10201 gcgagggaag ataaaccgcg aagcggaggc gcgtcaagcc gcaggacgcg tcgaaattgt
    10261 agagcctagg gcaccggcac ggaaaacgtc acttccgtcc cgcgttcgtt gtaggtcaaa
    10321 tcggggaaaa acgccttggc aagaaaaacc ccccggccgt tgggatggcg actgtcgcag
    10381 ggctgttcag gtgtggccac aaaacggctc caggcaaagc cctttccttc gtccgtgatc
    10441 gcataacgga ggcgccggcg agttttgtca tgggaggcct gcaccacgac acgccgcctg
    10501 gcaaaccgcg ggtggcggcg gcgctcctca atcagccgct caaactggtc gctgctcaat
    10561 gccgcgtgtt tttcctggta caggatctcg agactgccat gttctacggc gttgacgatg
    10621 agttcgatca aggtggtgcg aagatgcagg cgctgcatct cgggaagcgt cagggcggtc
    10681 tgctgaatca accaggtcac acaggcctcg acatgatccg gattggtgcc taacaccaga
    10741 cggtagtcca cctgttcgac gccagcgata ttttcgacgg tcgacggaag ctgtcgaagc
    10801 gcccgatcca gcgccaagcc cagttcttcc acgagcaggg gcttccgtag atagtcccca
    10861 gcgcccgccc gtactacgtt caggatcgtc tcagcatctc cagactcgcc ggtcgcaagg
    10921 atggctgttt tcggggaccg catgcggagt tcacggatca gcaacacacc ggcctggtca
    10981 gccgggaaca gatcagtcat gaggatatcg ggggcagcca tatcaatcat ggccaatgcc
    11041 acttgcggat ccggcgctgt aatgacggag atccctcgga gtttcccctc ctccagggcc
    11101 attgcccagg tctcgggaga ggcatcgacg accagcatcg tgggcgatgc ggcgtgacca
    11161 atcatgaggg aaagcctttt tgcatgatct cagccagtgc ggccgtcagg cgttcgactt
    11221 ctgcacgagt ctgctcagcc agcgccggga tgtcctgcgc ggcggccttt cgggcggctt
    11281 cttcaagatg tgcagctgca gcgacagcgg gacgcgcaca aaactccatg gccgaccctt
    11341 ttaatttatg tgcttccttg accagcaccg gaaggtcctg ggccgacaga gcggtctgaa
    11401 gtgcggccag cgcctgcccc gagcgttcca caaagaggcc ggcaagggtc agaaacaaat
    11461 cctgatcccc gtcgaagcga tcgagggcgg cagccaggtc catgaccggt tgcgagtcca
    11521 ttacgccctt tccttttggc ccaacagagt atccagcatc tctaacccca gcagagcgac
    11581 gtcatcgggg aaaatctccc ctgccagcca ctgtcgggcg gtggccatgg tgtcagcgat
    11641 gctttgtcca agactgtggc tccggtgcgc ttccagcgtt gcttgcaggc ggtctcgtcc
    11701 ccagagttcc cctgtcggtg aaggggcctc atagatgccg tcactcagca gatacagacg
    11761 atccagcgac agcaacgggg tatgcatgct ttcaaacaca ctgtgcggat cgaatcccaa
    11821 gggaagactg gccatggaga gccatcggga tccatcgccg gctgcctgca gaaatgctcc
    11881 gctatggcct gcggtggcgt aggacagcat acgtgaggag atgtccagac gccccaccca
    11941 taaggtgaaa tattctccgt cctgcgtcag cggaaacaga cgattcgcct ccgtaatgat
    12001 cgctcccgga tcaaaacttc cgaccacttt cgccagattg tcttcacgga gaaaactcat
    12061 aagtcccact gctcgcaagg cggcagccac tccgtgcccc gacgcgtcga ggatgtataa
    12121 ccctagagca tcggctcccc aggagcacac ttgaaacaag tcgccgccca gggcaagaga
    12181 tggctggtag gcccactgca tactgactcc cgtggccggc atgccgggca gagggagttg
    12241 cgcgcggacg aaatccgcgg ctgattgcag ctcgctttcc aactccgctt gtttcgccga
    12301 cagcagacgg tgagagcgat cgaggtcgtc gcgcgtgtgt tcgatttcac gtaccagggc
    12361 gctcgaccga aggctggctt gcacccgcgc caatacttcc tgtttgctcg ccgctttgct
    12421 caggaagtca tcggccccgc gggagagccc ttccgcgatc tgttcgggtc ggtcgtgtgc
    12481 cgtcatgagc acgacctggc tggatttcaa ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnntcgatac gtcgtgcggg ttcagccacg tgtcgtgctc ctgatgtgtc
    12661 ccttacccgg ctttgggcaa ccggcaggca gccagtgcgt cctgctccga atggtagacc
    12721 ggcagcagtt gatgcaaatt cgccagggtc aggatttctt tgacataact ctgggggttc
    12781 aggagaccga tcgtgccccg cgtcgcgctg aggctttgcg tcagtaacgc cagtgtcccg
    12841 agcgccgaac tgtcgaggaa ccgaacgcct tgcatattca aaatcagatg acggacgccg
    12901 ccggctctgc tgcgctccac cgacgctttg aacgtggctc gattcgcata cgtcagttcg
    12961 cccaccaagt cgagaatgac ggactccccg actcgccgtt cagtaatctg catgcctgct
    13021 tcctctcatc tttagaaaag aagggtcacg cgcttttctt cgtcacagtg gaagctggcg
    13081 attcgatcag cggaatggtc ttgtgcagat tcgccagctc gatgatctgc cgcaccgtcg
    13141 cctgaggata ggccaacgac aaggtgcgct tttcggcctg caactgccga tgagaaatca
    13201 tcaggagtcc caacgcggcg ctgtcgagga acgccacctg cgacagatcg atgacgatgt
    13261 gttccatggg atcgacgctg acggacttca acatttcctg aaacggtttc cgctgggtat
    13321 aggtaaagtt gccttccagt tttaaggtca ctttatgatt gtggatttcg ctggtgattt
    13381 tcattgctgc tcctcttagt gcaaaaggtg aacgatcagg cacgggtgac ttgagtcaaa
    13441 atcgctgacg cgatgtcgcc aagcggacgg acgatgtcgg cggcaccaag cttaatggct
    13501 tctttcggca tgccgaacac cacgcaggag gcctcgtcct gggcgatggt aaaggcgccg
    13561 gcttctttca tgcgtaacaa ccctttggca ccgtctccgc ccatgccggt gaggatcacg
    13621 ccgaccgcgt tgcctcctgc atactgggcg acggagtcga acatcacatc gaccgagggg
    13681 cgatggcgat tgaccggttc atcctggttg atgcggaccg aatagcgcgc gccgctgcga
    13741 accagggtca tgtggtagtt gcccggcgcc acgagcgcat gccccggcag cacgctatcc
    13801 ccatcctcgg cttctttgac ggagatgcgg cagagctgat tcatgcgatc tgcccaggtc
    13861 ttcgtaaacc gttccggcat gtgctgggtg atgaggatcg gcggggtatt gggagggagc
    13921 acctggagca cgtctttgac cgcctctgtc ccacccgtgg aggagccgat cgcaataatc
    13981 atatccgtgg tcttgatcat ggccgaattg agagatgtcg gacgcgaggt gctcgatgcc
    14041 gccgccgtgg tgggacgcac ggaggccgtt gcggcagcct tgattttggc aatgagatcc
    14101 tgcgccacct ggtccatgcc ttcgcgcaaa tcgattttcg gtttcgtgat gaaatcgacc
    14161 gcaccgagtt ccagcgcccg gagagtcgtt tggcacccag cctcagtgag cgagctgacc
    14221 atgattaccg gcgtcgggcg tccgcgcatc agcttttcga gaaacgtgag cccgtccatt
    14281 ttgggcattt ccacatcaag ggtcagcaca tcgggattca acgccttaat tttttcccgg
    14341 gcgacatagg gatccggtgc gctgccgatc acttcgatgc caggatcttt tgacaacaac
    14401 tcagccaaca cctgtcgcat cagtgcggaa tcatcgatag tcaggactcg aatcttagcc
    14461 attgccacac atctcctggt cttctggtgt gcgcatgttc tgtgccgcca ccggtctcct
    14521 gtgtcaaaac aacgtaatat tgtcttgttg caccgtcggt tcgacttcga tccgatgctg
    14581 gtattccgtc tcccgctcgt agatcgtgcg gtttttcacg cgctcgatct tcttcatcag
    14641 gacgcggccc gagtcggtga agtagtacac tttcctcgga tacacatcgc ccaggtcgct
    14701 tttcgcgagg gcgtagcctt ccgtcttcaa atattggagc acccattccg tgttgcgtgc
    14761 cccgacgtcg atgttaccgt catagatctt ccctgccccg aataatttca cttccagacg
    14821 gtgccggagt ccgccgcgtt tcaaaatttc gttgatcagc tgttccatcg cgaaggaccc
    14881 atagcgggtc gattctccgc cccaggcatc gccggactga tgctccttcg gagccggaag
    14941 catgaagtga ttcatgccgc cgacccccac gatgggatcc cggatacagg cggagatgca
    15001 ggaaccaagg accgtataga cgaccatcgg ttcccggctc acgaaaaact ctcccggcag
    15061 aatgcacgcc acctcgtggg ggaatcgtcc atcgcgcatg cgccggatat gcgagaacgc
    15121 atcggtgtcg atgacggcca tgtctctcag gattccttcc aatagatcgt cggcgcgatg
    15181 gttttgaagg gatgagacac ccactgcaaa ctttccgagt ggccgatgaa gagatgccca
    15241 cccggtttga gatggtgata aaacttgttg accaggcgtt cctgtgtcgg gcgatcgaaa
    15301 taaatcatga cattccggca gaaaatcaca tccagcggcg ctttgatggg aaaatgatcg
    15361 tccatcaggt tcaaccgccg gaacttgatg atggcggaga ggtggggctt caccttcacc
    15421 agtcccgcac tatctccccg tccctgaagg aaatgatgtt tgacgatttc gggcggcacc
    15481 tcgcgcaccc ggtcttccgc atacagcccc ttggcagcat gggccagaac tcgtgtcgaa
    15541 atgtccgaag ccagaatttg gaagttccac tgggccggct gctggacccc ttcgtgaagg
    15601 gtcatggcaa tggtgtaggg ttcctcgccg gtcgagcagg ctgaagacca gatgcggata
    15661 cgcttctcct tctcgagaga aggaagaatg cgctcgcgca gaaaatcgaa atgcttcggt
    15721 tcgcgaaaga agtcggtctt gttggtcgag agcagatcga gcatgcgcgt aaattcttcg
    15781 ccgctgttgt cctccgtcac atactcatag tacgaggaga aatcgcccaa ttggaggtcg
    15841 cgcaaccgct ttgtgaggcg tgacaccacg agtgactgtt tgctctctcc gagagagatg
    15901 ccgctctcgt catagagcag ggtccggatt tgatcgtact ctttcttcga aatgggatag
    15961 tccatcgtct cagctccgat accagacaaa cagcatgaga tatacgaggt aggccgtgac
    16021 catcgtgccg ctaaacgtcc agataagcgc gttgcccatt cggcgatgcg tggtcatgcc
    16081 ggccaccatg gctccgacgc ttccgaaccg tagccggtgg agccccatat acaggttgta
    16141 cgcacccagg ccaatagtgg tgacagcgag cagcatgtgc gtgacgaaaa ttgggacgta
    16201 caccgaccaa taggcacttt ccggcccctg gaaggactct cgtccaaaca gggcttgttt
    16261 caggacgtag gcgaccagcc agataccgac gatcgtgcaa ccaacaatca tgcgatgaga
    16321 atgatgcgtg acatcgtgag acttggccgc tttgacgccg gctaacacga cgagatacgc
    16381 gacagtcaga ctggacagga cgaggtacca aagaagcgtt ttgacgtcca tgatcgtcct
    16441 cgggctagag gtggaatcga ccggaatctg tgtatagctc cggtgtgagt acacccgcac
    16501 gttctcttat cggttgctga ttcgcgtttc ttgaggagaa ggaagctggt actgatgaag
    16561 gaaggaaggt tgggctgcga aaacggtagc accgcgggag tgggcagatt ctgcccactc
    16621 ccgcggtgca ggttcctaga actcttcgaa gtcatcgtcc tttttgcgac gatcgtgtcc
    16681 gttgccggcc gcgacgcctg cgggttgctt cgcttcagcc ggctttcccg gcacgcctgg
    16741 tttcttcagc cccagcttgg gggctgaggc gaccggcttg acggccgact tcacactaga
    16801 cgcgactcgc gccggtgcct ggttc
//
LOCUS       DAOQEW010000073        16777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_912_length_16777_cov_8.454910, whole genome shotgun sequence.
ACCESSION   DAOQEW010000073 DAOQEW010000000
VERSION     DAOQEW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16777
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_912_length_16777_cov_8.454910"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..566)
                     /locus_tag="PLS35_09610"
     CDS             complement(150..566)
                     /locus_tag="PLS35_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18962.1"
                     /translation="MMYSRSHGAVVLSLSLLLLSACATEETMTTGGSTPAASASASMK
                     TTAQAYDSLQSCLDRIPSGGTAGNRMIAEESCRRDETFRQSIAGNAATKSGNRYAAGA
                     AGDSLEACMARIPKDGSVGQRMLAEESCKRDQANQR"
     gene            complement(646..>1925)
                     /locus_tag="PLS35_09615"
     CDS             complement(646..>1925)
                     /locus_tag="PLS35_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNI18963.1"
                     /translation="ELVTATQAKQILPFEQAQISALRVRSHSGEVVLSQTPGHPWGIT
                     APIQTDADQRQVQALLRALVTGKVSRLVETHPVSLTPFGLDHPSTVVTVTAGEREETL
                     SIGDAGPLSSTLYVLRASDEAVLLTDLAPKDFLNRTLLSFRRKELLQFNQQETEQLRL
                     TYPTTEMVLYGIEEKPKKKWKIRYPIEAEADRTEVQALLFRLEDLKALAIVDPGPERE
                     KLAPLLTKPKVKVTVHAAGTDQVVRLFQPDPASGEAYAQTAQDGPIYKISPSVIKELT
                     KDLFALQDKRLLGAETGDIALLSIKTRDEQYTLVHDRDGWMLEDQPNEKLRQDVADLL
                     VSRIVNLPAEERILKQAGPLAPYGLVAPVAEFIATGKNGRVAGRLALGNQSGGLVYAI
                     GQRIPGVFQVRPDLLTQIPSRLALLASGPATPP"
     assembly_gap    1926..2025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2026..3377)
                     /locus_tag="PLS35_09620"
     CDS             complement(<2026..3377)
                     /locus_tag="PLS35_09620"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNI18964.1"
                     /translation="MEMTRNPLPIGAIGTGLAIAGVIAYSLVPDKLWLVTLLEGLALA
                     CFIWAFATHFDQVKRFSSQRSTRMGANSALMVALFLAILAILNFLAARHSIRWDFSEN
                     QNYTLAPETYRVLRNLTRDVNVTVFTREKDPGYQGYKERFDSYRQASPKLTVQFIDPE
                     RQPKVAQNYGVTRTDVAIFESGPQSVRITSPAEVEITGALVRVSKDSKKRVVFLEGHG
                     ERSLEDKDRGGMALTKEVLEKQGYEIGTVTLLQETAVPSNTDVLVIAGPRRAITKEEQ
                     DRIQTYVAQGGHLLLLLDPDTQASMDTLLKIWGLELGAGVLVDLQDRLAQGDLTALLV
                     RTFTDHEITHELTAAVLFPLARHLTFHEEQAKDWDYVPLARTSPRSWAETDMKGRVVS
                     YDEKEDVQGPLALAAALTPKQAPEEGKPRPAVVVVGNSAFASNGFINFVGNSDFFQRT
                     V"
     gene            complement(3368..4138)
                     /locus_tag="PLS35_09625"
     CDS             complement(3368..4138)
                     /locus_tag="PLS35_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNI18965.1"
                     /translation="MPPVQAIIRKELRSYFVSPIVYVVGGVFLLTFGFLAYLYIVFTG
                     AQAIQLMQMQGGTAQINLNDLVFRNLFASMRFVLLLILPILTMRLLAEERKLRTFEFL
                     MTAPIRVSEIIVGKFISVYLVFMGMLLLTTLVPLTLALFSDFDWYPVFTGYLGLLLLG
                     GFFLAVGLFASSVTENQIVAAFTSFGLLLMCWLLAGLGSLLGDTPVGQVVSYLSFMEH
                     YDHLVRGLIDTKDLMYYVSGTVLMLFLAHRIVDASRWK"
     gene            complement(4142..5110)
                     /locus_tag="PLS35_09630"
     CDS             complement(4142..5110)
                     /locus_tag="PLS35_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI18966.1"
                     /translation="MIEVCNITKRYGHLTAIDRVTFRVEKGEVLAFLGPNGAGKTTTM
                     RILTSFIPATEGTAHVAGFDCAEQPDEVKKRIGYLPETPPVYQELTVAEYLGFVGKLR
                     GLSGTGLTQALERVTERLSLGTVRQRLIANLSRGYRQRVGLAQALIHDPPVLILDEPT
                     VGLDPKQIIEIRELIKSLAGSHSVILSTHILPEATAVCQRVVIINGGRIVAEDTPDQL
                     SARLRKSEKISVTLKAPPVNLAERFREVPGIEHVLTTADPHTVLIECALGRDIREDVA
                     RFAVGQNWGLLEMKPVSMTLEDVFLKLTQREDEPPKSNDTTPGEHH"
     gene            5365..5730
                     /locus_tag="PLS35_09635"
     CDS             5365..5730
                     /locus_tag="PLS35_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18967.1"
                     /translation="MNQARPSLLGVLVIGAALCVALAGLGSFLTSVYAASNTVHEIKG
                     TVENVHAHEDPPVIVVKGQHGSSEAIVVGAVVKQGATIMRGKKRITLDQIHAGDKVTL
                     KYVKTRDGLTVRSIALHRN"
     gene            5787..6443
                     /locus_tag="PLS35_09640"
     CDS             5787..6443
                     /locus_tag="PLS35_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18968.1"
                     /translation="MIRQHVVRGMSAPVAVGLLLLMGCSGKGDIIPMNLTPKAKEMTN
                     ASSAVKPMAGPKVVVIPFEDARADHAKVGSRSSMWGGETDFHVSSGSAGEETGQALAD
                     YLKRKGWLVQYQKAAPPESENGPNIVLSGKILDLTVDAKRGFLMTDIEARSKLVIQAN
                     NREDGSSIVRTDAHSGSHDNVFWFDPQDGEELLGEVLEKNFERFVLNTKLEDRSIRFR
                     "
     gene            6783..7442
                     /locus_tag="PLS35_09645"
     CDS             6783..7442
                     /locus_tag="PLS35_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNI18969.1"
                     /translation="MVRNFWNRSRCKTGSALAPLVACLLLSACSALPKGDLPLDLGIK
                     DRGVASWYGKEFHGKLAANGEVFDMTAYTAAHRKLPLGSLVRVVNLANGKSVQVRIND
                     RGPYVSGRMLDLSQAAARELGMLEAGTSPVQIEVIGNHRPVAPIPSSLIASVAGMLLN
                     TDTRVMKRHTRQEEGVEVPVVPLRMMPQEALYVRRERRIGSMLAADHSAHNTVPGLIV
                     S"
     gene            7574..7774
                     /locus_tag="PLS35_09650"
     CDS             7574..7774
                     /locus_tag="PLS35_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18970.1"
                     /translation="MAKKPTGESDEVRLKKKIAVSTKDESKSGDPAVRSLRKRLKRVQ
                     RKRRALVLRKKHAAGKQAEAKP"
     gene            7838..>8660
                     /locus_tag="PLS35_09655"
     CDS             7838..>8660
                     /locus_tag="PLS35_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18971.1"
                     /translation="MEHTGEKKDQAQAVSPVGPVEDEALTDQVSDQLAASLDSSVGEQ
                     ELIEPMQEVVLEEEKVKDEIDIQIDLLKDPDWVVRREAAITLGEMGDERCVEPLAAAL
                     RDGDWQVREVAIEGIGQIGSPAVDVLLKLLRDWDVRKSAIMALGKIRDERVLEPLMQQ
                     LRNDEFMEDATDALVNLGEPALPGLIKALKDKEELVRKQAVIALGRIKSPEAIDPLIE
                     MLQNKDWFTRLTAAAALEAIGDERGREAIKPLLKDTDMVVKMRVERILAKWKKQPA"
     assembly_gap    8661..8760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8934..10187)
                     /locus_tag="PLS35_09660"
     CDS             complement(8934..10187)
                     /locus_tag="PLS35_09660"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI18972.1"
                     /translation="MTFYRAAEYSRVIDLIQHPPTGQEPSLEAIRYAILSYVKMGKPE
                     EAWKLYPKLTAGDRPDDPALLREIARAFIVSRVRDSQEHIRIAAYTALAEMGEADTLP
                     LLEDGLLDSSALVRARAAEAIGRSGLAGRSAALKRALRDEAPGVRIAAINALSDAKVS
                     GITDQLTEIARVDEGPESIFAFAGLYKLGRTDVLTDISSAVTLPDPEVRMAALGVLGR
                     LKRPASLSILSQAVYDPHPAVRAFAAGALGEFGSIEGIAPLTHALGDEDPRVRSVAAS
                     SLGRLGVSESRSVVQPLLRDSDEHVRISAVESLLRLGDTGVILNAAALARHPDPSIRG
                     DTAHALALATTANALPILENLLRDQQPLPRLMAARALGKSPLKSLIPAAKIGLQDSDA
                     AVRITSAGSLLRILSRKDKVSTRKG"
     gene            complement(10340..12499)
                     /gene="glgP"
                     /locus_tag="PLS35_09665"
     CDS             complement(10340..12499)
                     /gene="glgP"
                     /locus_tag="PLS35_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNI18973.1"
                     /translation="MQAPDHIQPPQDMVPQSLHRLGELARNLWWSWNQPARQLFESID
                     PTLWFLTHHNPVQLLAGVKPERFATLASDPTFARQYSAVLRSFDEYMSSKNTWAATEF
                     PALQNSPIAYFSAEFGLHISIPIYSGGLGILAGDHCKEASDLGIPLVGIGFMYPQGYF
                     KQRINPEGWQEATYVPFNRYDSPIHQALTPAGVPCTIKVQIGHRQVTVLVWQVRNGRM
                     SLYLIDTDVPENTPEDRALSARLYGGDQETRLCQEFLLGIGGVRVLRALGITPAVWHC
                     NEGHSAFLTLERIREFVSTGHSHAEASELVRQSTVFTTHTPVPAGHDIFPFHLMDRYF
                     HNYWGQLGLSREEFLRLGETPESAGHGFNMTALAMRLSAHVNGVSREHGRVSRQMWQH
                     LWPGLAQDLVPIRSLTNGIHAPTWISPESNSLYAKYLSPDWAERIDDPTIWQRVTDLP
                     DDALWAVRQTTRRKLMRFIRERARDGWIRGHLQPMQAIARGTLLDPEALTIGFARRFA
                     TYKRATLLFRDLERLKQLLHNRWRPVQIVFAGKAHPADEPGRYFIHEVLNFCNDHKLG
                     GHIAFLEDYDMHMAKYLVQGVDVWLNTPRAPLEASGTSGQKAALNGVINLSVLDGWWQ
                     EGYNGANGWGIQPLPEGSDTQAQDAHDAEQLFRLLEQEVVPLFYQRDLDGIPRGWLHI
                     VKESIKTVAPRFCTKRMVKEYMRQLYAPATARTPNKW"
     gene            complement(12569..13405)
                     /locus_tag="PLS35_09670"
     CDS             complement(12569..13405)
                     /locus_tag="PLS35_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18974.1"
                     /translation="MTTKSHSFAQQMRTVAELMLAVSGESVSVIKRAAPEQALKLKRQ
                     DEWGIYLEFLRAMFNLTDRVSALHIPLREQPQFMDELTDTVIDQLKKVLEPAFGAGDD
                     QMEIVMTIGAAVSESRQTYERYRFLVTEESPAKNDLYRDFSDRVARAVGASGNPKVEA
                     AATLCIAAVIPALIGIFEGKTPAVPADATGNANPSAASSNPRGATGADIKLVSVMSSI
                     KGEEVETRWGLHPRFRQDLTQEEAKQLTATMNRVAKILGERYATVAFSDQWASWHKAG
                     HA"
     gene            13525..>15513
                     /locus_tag="PLS35_09675"
     CDS             13525..>15513
                     /locus_tag="PLS35_09675"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI18975.1"
                     /translation="MLYSATAPVMSLTAQSDANMDRDTKPYILKRTPDQDVVRKFSLD
                     YAKELNPQQYAAVTAADGPALVIAGAGSGKTRTLVHRVAYLIDSGVDPSQILLLTFTR
                     KSAEEMLERVGALIGSRSQRVCGGTFHSVANMLLRRYGRVLGIEPSFTIMDRGDAEDL
                     IALLRAQLGLNEKDKRFPRKGTIAEIYSKCENTLRGLEEIVLDEFSHFADHLDALGKL
                     QRAYQLAKRQRQLLDYDDLLVLLRDLMTQDEASRQAISRQFRYILVDEYQDTNRLQAE
                     LIRKLAATHDNVMVVGDDSQSIYAFRGATFRNIMEFPSWFPGATIYKLEENYRSTQPI
                     LSLANEIIQEAPEKYTKHLFTRKLDGPLPALVEAAGENAQSRFVAQKILELREEGVPL
                     NEIAVLFRSSFHSFDLEIELSRCGLPFVKRGGIKFIEAAHVKDLLAHLRVVVNPHDAV
                     SWHRVLMLVEGVGPKKAQDLVAAMVRVTDPYQVLRDSSGRSGKGLKELAVVLENLSKS
                     DDLSPTEQVNRVYEYYLPILKDHHDDYPKRIRDLDHLHTIAESYPGLTEFLADLALAP
                     PDGSAVGVEAAGSDDEQVVLSTIHSAKGLEWQCVFLLWVVDGKFPSVFSFNTDEELEE
                     ERRLLYVAVTRAKRYLFLTYPINVYDRSSGMLLSKPSRF"
     assembly_gap    15514..15613
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15614..16488
                     /locus_tag="PLS35_09680"
     CDS             <15614..16488
                     /locus_tag="PLS35_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18976.1"
                     /translation="VGGMLLVLLCSAPILAETPAAQPDASRLWSGLVAEAKALRLPTQ
                     FLEQVPKHFVTFEFEDLHAFAAEYHPADHRMVLDRSLSLNAAGRTLRPLGRLTHKELE
                     TLYHELFHVYMDFLEQEATPSEAHASLIAFAREQQHCRYQQVLITPVLQKRALKEERF
                     LSETESWEVLNETWAVFIGWAVWTQLEVDQKPRRDSRGPAHTSGWIARLDQAEQDAAL
                     IGYYEPEDLEEKRMARKRFLAPEFRLSSPELLRLMKDVLGSQPDVIRQATQVLQRSRP
                     VASTRGACAASTKP"
     gene            complement(16474..>16777)
                     /locus_tag="PLS35_09685"
     CDS             complement(16474..>16777)
                     /locus_tag="PLS35_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18977.1"
                     /translation="IRQQIYSLPPLSTWVPAREGPISKRKRATNQFKENKHQSVSFSG
                     SENEVSPTMHSSPAKRQGWIADKVRVDLASKAADFICDAFYCQGGMFYAGTILRLR"
BASE COUNT         3458 a         4323 c         4985 g         3711 t
ORIGIN      
        1 gtttaggaaa ccgctgctct atccaactga gctacggggg cgtcgtcgta cgatactgaa
       61 agacggtgag attgtctaca gcgaggcgcg cctccgaagc aggtgatcac ctccggaggc
      121 gcagcattca tcgaaatcgg aacgtcggat tatcgctggt tggcttgatc gcgcttgcag
      181 ctctcttcgg ccagcatgcg ctgacccact gagccgtctt tcgggatgcg agccatacag
      241 gcttccaagc tatcgccggc ggcaccggct gcgtagcggt ttccgctctt ggttgccgcg
      301 ttgcccgcga tggactggcg gaacgtctca tctcggcggc agctttcttc tgcaatcatg
      361 cgatttccag ccgtgcctcc ggatgggatg cggtccaggc aggactgcag cgagtcgtag
      421 gcctgggcgg tggttttcat cgacgcactc gccgaggcgg caggtgtcga tccaccggtc
      481 gtcatggttt cttccgtcgc gcaggcactg agcaaaagca gagataagga cagaacaacg
      541 gcaccgtggg aacgcgagta catcattgaa ggatcctcct tgatcaagag cgatgggcgc
      601 acagtagcat aatccccctc ttcctggaaa cactctcgtc gcggtttagg gcggtgttgc
      661 cgggccgctt gcgagcaggg ccaacctgga agggatttgg gtcagcaggt caggacggac
      721 ctggaatacg cctggtatcc gctggccaat ggcatacacg aggcctcctg attgattgcc
      781 cagggccaga cgtccggcca cacgaccgtt tttgccggtc gcgatgaatt cggccacggg
      841 ggcaacgagt ccatagggtg ccaatgggcc cgcttgcttg agaatacgtt cttccgcggg
      901 gagattcaca atacggctga cgagcagatc ggccacgtct tggcgcagtt tttcattcgg
      961 ctgatcttcc agcatccatc cgtctcggtc atgaaccagc gtgtattgtt cgtctcgcgt
     1021 tttaatggag agcagggcga tgtcgcccgt ttcggctccg agcagccgtt tgtcctgcag
     1081 ggcaaacagg tctttggtca attctttaat gacggaggga ctgattttat agatagggcc
     1141 atcctgagcg gtttgcgcgt aggcttcgcc gctagccggg tcgggctgaa agaggcgaac
     1201 gacctggtcg gttccggccg catgcacggt gactttcacc ttgggtttgg tcagtaacgg
     1261 ggcgagcttt tcccgttcgg gccccggatc cacgatggcc aaggctttga gatcttccag
     1321 gcgaaataga agcgcttgga cttccgtgcg atctgcttca gcctcaatgg ggtaacggat
     1381 cttccatttt ttcttcggtt tctcctcaat cccgtacagc accatttccg tggtcgggta
     1441 ggtgagacgg agttgctcag tctcctgttg attgaattgc agcaactctt tgcgccggaa
     1501 ggagagcagg gtccggttca gaaagtcttt cggcgccagg tcggtcagaa ggacagcctc
     1561 atctgacgct cgcaatacat agagggtgga cgagagcggg ccggcatccc cgatcgagag
     1621 tgtttcctcg cgctcgcctg ctgtcacggt gacgacggtg gaggggtggt ccagaccgaa
     1681 cggcgtcaat gagaccgggt gagtttcaac cagacgcgag acttttcctg tgacgagcgc
     1741 ccgcagcagt gcctgaacct gacgctgatc ggcgtcggtt tgaatcggtg ccgtaatgcc
     1801 ccagggatgg ccaggcgtct gactgaggac cacttccccc gagtggctgc ggacccgtaa
     1861 ggcgctgatc tgagcttgct caaacggcag gatttgtttg gcctgggtcg cggtgacaag
     1921 ttcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaccgt gcgttgaaag
     2041 aagtcgctgt ttccgacgaa gttgatgaag ccattgctgg cgaaggcaga gttgccaacc
     2101 acgacgacgg cgggacgggg cttcccttcc tcgggggctt gtttgggcgt caacgcagcg
     2161 gcgagcgcca agggcccttg cacatcttct ttttcgtcat agctgacgac ccgacccttc
     2221 atatctgttt ctgcccagct ccgcggtgac gtgcgcgcca atggaacgta gtcccagtcc
     2281 tttgcctgct cttcatgaaa ggtcagatgc cgggccaaag gaaataagac agccgcggtg
     2341 agttcgtggg tgatttcgtg gtcggtgaat gtgcgcacca gcagggctgt caggtcacct
     2401 tgtgcgagac ggtcttgcag atccacgagg acgccggctc ctaattccaa accccagatc
     2461 ttgaggaggg tatccatgct tgcttgtgtg tccggatcga gtagaagcag gaggtgaccg
     2521 ccttgtgcga catacgtctg aatgcggtcc tgttcttcct tcgtaatggc gcgtcggggc
     2581 ccggcaatca cgaggacgtc tgtgttgctc gggacagcag tctcttgcag caatgtcacg
     2641 gtgccaatct cgtacccctg cttttccagc acttccttgg tgagtgccat tccgccacgg
     2701 tccttgtctt ccaagctgcg ttccccgtgg ccttctagaa acactacccg ttttttgctg
     2761 tccttcgaca cgcgcacgag tgcgccggtg atctccacct ctgctggcga cgttatgcga
     2821 accgattgtg ggccactttc aaagatcgcc acatcggtgc gggtgacgcc gtaattctgt
     2881 gcgaccttcg gctggcgttc cggatcgatg aactgaacgg tcagcttcgg gctggcttgc
     2941 cgatagctgt cgaagcgttc cttgtagccc tggtacccag gatctttttc ccgcgtgaac
     3001 accgtcacgt tgacgtctcg ggtgagattg cggagaacgc gataggtttc cggtgcgagc
     3061 gtatagttct gattctctga aaagtcccag cggatggaat gtctggcggc gaggaaattc
     3121 aggatggcta agatggccag aaagagggca accatcaatg cactgtttgc gcccattcgc
     3181 gtggaccgtt gagaggagaa gcgtttgacc tggtcaaaat gtgtagcaaa cgcccaaata
     3241 aaacaggcca aggccagtcc ctccaggagg gtgacgagcc agagcttgtc cggaactaga
     3301 gagtaggcaa tcactccggc aatggccagc cctgttccga tggcaccgat aggaagggga
     3361 ttgcgtgtca tttccatcgc gaggcatcca caatccggtg agcgaggaac aacatcagta
     3421 cggttccgct gacgtaatac atcaggtctt tcgtgtcgat cagtccgcgc accaggtggt
     3481 catagtgctc catgaaggaa agataggaga cgacttgtcc tacgggcgtg tcgcccagga
     3541 gcgagcccag tccggccagc agccagcaca tcaggagcag cccgaagctg gtgaatgcgg
     3601 ccacaatctg gttttcggtg acagacgagg caaaaaggcc aacagcgaga aaaaacccgc
     3661 cgagcagtaa caagcccaag tagccggtga atacggggta ccaatcaaaa tcgctgaaga
     3721 gtgccagcgt gagcggaacc aaggtcgtca acagcaacat ccccataaat acgaggtaga
     3781 cgctgataaa tttcccgacg atgatttcgc tgacacggat gggggcggtc atcaggaatt
     3841 caaacgttcg aagtttgcgt tcttcggcca gcagtcgcat ggtgaggatg ggaaggatca
     3901 gcagcagcac gaatcgcatg ctggcaaaaa gattgcggaa cacgagatca ttcagattga
     3961 tctgcgccgt gccgccttgc atttgcatga gttgaatggc ctgggctccg gtgaagacaa
     4021 tgtagagata ggcgaggaat ccgaaggtca ggagaaagac tccgccgacg acatacacga
     4081 tcggcgagac gaaataggag cgcaactctt ttctaatgat cgcttggacg ggtggcatcg
     4141 cttagtggtg ctcgcctggg gtggtgtcgt tcgacttcgg cggctcgtct tcccgttggg
     4201 tcagcttcag gaagacatct tccaatgtca tcgagacggg tttcatttcc aatagccccc
     4261 agttttggcc gacggcaaag cgcgccacgt cctcccggat atcccgcccc agcgcgcatt
     4321 cgatgagcac cgtgtgcggg tcggctgtgg tcagcacatg ttctattcct gggacctcgc
     4381 gaaatcgctc cgccagattg accggcggag ctttcagggt gacgctgatc ttttccgact
     4441 tccggaggcg ggcggagagt tggtcaggag tgtcttcggc gacgatccgc cccccattga
     4501 tgatcacgac gcgctgacag actgcggtgg cttcgggcag gatatgcgtg ctgagaatca
     4561 ccgagtgcga gccggccagg ctcttgatca attcacggat ttcaatgatc tgcttcggat
     4621 cgaggccaac cgtgggttcg tccagaatga ggacgggagg atcgtgaatc aaggcttggg
     4681 ccagaccaac ccgttgacga taaccgcgtg acagattggc aataagtcgc tgccggacag
     4741 tccccaaaga aagccgttcc gtgacccgtt ccagcgcctg cgttagacct gttccgctca
     4801 atccgcgcag cttccccaca aaacccaagt actcggcgac cgtgagttct tggtagacag
     4861 gcggagtttc cggcaaatat ccaattcgct tctttacctc gtcaggctgt tccgcgcaat
     4921 cgaagcccgc gacgtgcgcg gttccttccg ttgcgggaat gaaggaggta aggatacgca
     4981 tggtcgtggt tttgccggcg ccattagggc cgaggaaggc cagcacctct cctttttcca
     5041 cgcgaaaggt gacgcgatcg atcgcggtga ggtggccgta gcgtttggta atgttacaaa
     5101 cttcgatcat gcagtcggga ttgacggctg gctggtgtga ttcctgctgt tcgtttggta
     5161 cggcacgtgg tgtggcaaat cgcgcctgat attaccgacc gtgtgagatg cagtcaagag
     5221 aaggatacgt tgtgctgaag gacgtagaaa agcgtgagtc cgtctttaca gcgtgattcg
     5281 taactgctac agttccgcga ctttagccac cgggcggcct ggtggaatgc ctgacgaaga
     5341 ggtctctcct gaatgaagag tgacatgaac caagcgaggc cgagtttgct cggcgtactc
     5401 gtcatcggcg ccgcgttgtg cgtggctctt gccggacttg gaagtttcct gacatccgtg
     5461 tatgccgcca gtaataccgt ccatgagatc aaggggacgg ttgagaacgt gcatgcccac
     5521 gaagaccccc ctgtcatcgt cgtcaagggg cagcatgggt cgagtgaagc tatcgtggtc
     5581 ggggcagtgg ttaagcaagg cgccaccatt atgcgtggga aaaaacggat cacgctcgat
     5641 caaatccatg ctggagataa ggtgacgttg aagtatgtga agactcgcga tggtctgacg
     5701 gttcgatcca tcgcactcca tcgcaattaa tcacgcgcat tcattctcgt gacgttgtgt
     5761 ttttcacaag gaggtagaga gattttatga ttcgacagca tgtggtgcgg gggatgtctg
     5821 cgccggtagc cgtcgggttg ctgctcctca tgggctgctc cgggaaaggc gatatcattc
     5881 cgatgaattt gacccccaag gccaaggaga tgaccaacgc ctcttcagcg gttaagccga
     5941 tggcgggacc gaaagttgtg gtcattccat ttgaagacgc gcgggccgac catgccaagg
     6001 tgggaagtcg aagctccatg tggggcggtg aaacagactt tcatgtctcg agcggcagtg
     6061 ccggtgagga aaccggtcaa gcacttgccg actatctcaa gcgcaaaggc tggctggtgc
     6121 agtatcagaa ggctgcccct ccggaatcag aaaatggtcc caatatcgtg ttgtccggaa
     6181 aaattctcga cctgacggtc gatgcgaagc ggggatttct catgactgat attgaggccc
     6241 ggagcaagtt ggttatccag gccaacaatc gggaagatgg cagttcgatc gttcgaaccg
     6301 atgcgcattc cggtagccat gacaatgtat tttggtttga tccccaggac ggggaagagc
     6361 ttctcggtga agtgctggaa aagaattttg agcgatttgt tttgaacacg aaactggaag
     6421 atcggtccat tcggtttcgg tagagagcaa tccggagttg gggcgcttta ggtcctgaca
     6481 gatcgtgacg ttccgtcctt ttgatctctg gggggacgtt catttgtgaa ctgtactgtc
     6541 gtgtaaagac ttagagttct ggcagtgctg gctcatttga aggcagttcg gtctttagtc
     6601 gcgtatttcc ggcgggaaaa accgcaactt gtgttcagtg atgcaaggtt cgttgcccat
     6661 ttgtaggggg ccttgctacc cagacggtat tgtcgccaag gggaatctcc cgtagcctga
     6721 aatcactttt gaggagctgt tccagcagac agtgatttca ggctttcaaa cgaggtgctt
     6781 ctatggtcag gaatttctgg aaccgttctc gttgtaagac gggatctgct cttgctcccc
     6841 tggtagcctg cctcctcctc agtgcctgtt cagcccttcc caaaggcgat ctccccctgg
     6901 atctcggcat caaggatcga ggcgttgcgt cctggtatgg aaaggagttt cacgggaaac
     6961 ttgcagcgaa cggggaagtc tttgacatga ccgcctatac ggctgctcat cgtaagttgc
     7021 ctcttggcag tctcgtgcga gtcgtcaacc tggccaatgg aaaatcggtt caggttcgaa
     7081 ttaatgatcg ggggccatat gtgtcaggcc gaatgctgga cctttcgcaa gccgctgctc
     7141 gtgaactggg tatgctggaa gccggcacta gccctgtgca gatagaagtc atcggcaatc
     7201 atcgtccggt ggcaccgata ccctcttcac tgatagcctc cgtagctggt atgttgttga
     7261 ataccgatac gcgagtcatg aagcggcata cgcgccagga agagggggta gaagtgcctg
     7321 tcgtacctct ccgaatgatg ccccaagaag cgttgtacgt tcgccgtgaa cggcggatcg
     7381 gcagtatgtt agcggcagac cattcggcgc ataataccgt ccccgggctc atcgtttcct
     7441 gatacgagca atcgacattc cctcagttga cacctcctcc tgcacttgat tagactgccc
     7501 gatctagatt ggcatgctga gcatgccgct atttccgttg atgttgatcc taactgttgg
     7561 aggttgacgc tgaatggcaa agaaaccgac cggtgaatca gacgaagtcc gactgaagaa
     7621 gaaaatcgcc gttagcacta aagacgagag caaatctggt gatccagccg tgcggtcctt
     7681 gcgtaaacga ctcaagcggg tgcagcgtaa acgacgagca ctcgtgttaa ggaaaaagca
     7741 tgcggcaggg aaacaggctg aagcaaaacc ctgatgaatg gcggtatcga agcatctgac
     7801 cctactccaa ccctcctgcg gaataaggat tcttgatatg gaacatactg gcgaaaaaaa
     7861 agatcaagca caggctgttt cccctgtcgg cccagtcgaa gacgaggctt tgacagatca
     7921 agtctctgac caacttgccg catccttgga ttcctccgtg ggggagcagg agctcattga
     7981 gccaatgcag gaagtcgtgc tggaggagga gaaggtcaag gatgaaatcg atattcagat
     8041 cgacctcctc aaagatcctg attgggttgt gcggcgggaa gcggcaatta cgctgggcga
     8101 gatgggggac gaacgttgcg ttgaacccct cgcggcagcc cttcgtgacg gtgactggca
     8161 agtgcgagag gtcgccattg agggaatcgg acaaatcgga tctcctgccg ttgatgtact
     8221 tctcaagctt ttgcgcgatt gggatgtgcg gaagtctgcg atcatggcat tgggaaagat
     8281 tcgtgatgag cgggtgttgg agccgctcat gcagcagctt cggaatgatg aattcatgga
     8341 agatgcgacc gatgccctgg taaaccttgg cgagcccgcg ttgcccgggc tgatcaaggc
     8401 cctaaaggac aaagaggagt tagtgcggaa gcaagccgtg atagcgttgg gccgcattaa
     8461 gtcgccagaa gccatcgatc cgttgatcga gatgttgcag aacaaagatt ggtttacacg
     8521 gctgacggcg gcggccgcgc tcgaagctat cggggatgaa cgtggtcgcg aagcgattaa
     8581 gcccttgctg aaagatacgg atatggtggt caagatgcgg gtcgagcgca ttcttgccaa
     8641 atggaagaag cagccagccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 cgcggcgccc cgccgcgttg atgctgttgg gatcgcacaa aatattcaat ctgttgtcag
     8821 gtttatttat tggaaaacct gagccaattt gtcgagcctc ggggttggcc ggtttttgcg
     8881 gactttcgaa gttgagcgaa aaaacccagc tgatgagggc tgagcatgtc ggctcagccc
     8941 ttacgggtgc tgactttgtc ttttcgagag agtatgcgaa ggagacttcc ggcggaagta
     9001 atacgtacgg ccgcatcaga atcttgcaag ccaattttag cggcaggaat gagcgatttt
     9061 agcggagact ttcccagtgc gcgggctgcc atgagccgag gaagcggttg ctggtctcgt
     9121 agcaggtttt ccagaatcgg gagcgcattg gcggtggtcg cgagggcgag cgcgtgggcg
     9181 gtatcgccgc gtatggatgg atcgggatgc cgcgcgaggg ctgccgcgtt caaaatgaca
     9241 ccggtatcgc ctaagcggag cagtgattcc actgcgctaa ttcgcacatg ttcgtctgag
     9301 tctcgtaaca gcggctgaac gacgcttcgt gattctgaga ctcctaggcg accgaggctg
     9361 gaggccgcaa cgctgcggac gcgcggatct tcatctccca gggcgtgcgt cagtggcgcg
     9421 atgccttcga tactcccgaa ttctcccaag gcgcctgcgg caaaggcacg cacggcagga
     9481 tgaggatcat acacggcctg actgaggatc gagaggctgg cagggcgctt caagcgcccc
     9541 aataccccca aggccgccat acgtacttcg gggtcaggaa gagtcaccgc actcgaaata
     9601 tccgtcagca catctgtgcg gccaagtttg tacaggcctg caaacgcgaa gatcgattct
     9661 ggtccctcat cgacgcgggc aatttcggtc agttgatcgg taatacctga caccttggca
     9721 tcgctgagag cattgattgc cgcaatacgc acgccgggcg cttcatctcg aagcgcgcgt
     9781 ttgagcgcgg ccgaacgtcc tgccagtcca ctccgcccaa tggcttccgc agccctggcc
     9841 cggacaagtg ccgaagaatc caacagtccg tcttcgagaa gggggagggt atcggcttcc
     9901 cccatttctg cgagtgccgt ataggccgcg atacggatat gttcttggga gtcacggact
     9961 cgggacacga taaatgctcg tgcgatttcc cgtaataacg ccgggtcatc agggcgatcc
    10021 ccagcggtaa gcttggggta aagtttccat gcctcttcag gttttcccat cttcacgtag
    10081 ctgaggatgg cgtaacgaat ggcttccagg cttggttctt ggccggttgg agggtgctga
    10141 ataagatcga tgacacggct gtactccgcc gcccgataga acgtcatggc atcccgttcc
    10201 agtggcgacg gcgaggtttt aggcgtggag gtcgctgccc ctaccggcag gggacttatc
    10261 cagactatta acaggagtcc tgcgggaaat gcccaggtgg agatgtagcg gcctggcatg
    10321 ggagaagacc taattcctgc taccatttgt tcggcgtacg ggcagtcgcc ggcgcataca
    10381 gctgccgcat gtattctttg accattcgtt tggtgcaaaa gcgcggagca accgtcttga
    10441 tgctctcttt gacaatgtga agccatccgc gcgggattcc atcgagatcc cgctgataaa
    10501 acagcggcac gacctcttgt tctagcaggc gaaagagttg ttcggcatca tgcgcatctt
    10561 gggcttgggt gtccgagcct tcagggagag gttgaatgcc ccatccattg gcgccgttgt
    10621 agccctcctg ccaccacccg tctaacacgc tgaggttgat cactccgttc agcgccgctt
    10681 tttgaccgct cgtgccactc gcttccaacg gcgcacgagg cgtattcagc cagacatcga
    10741 ccccttggac caaatacttg gccatatgca tgtcgtagtc ttcgagaaac gcgatatgcc
    10801 cgccgagctt gtgatcgtta cagaagttca ggacttcgtg aataaagtaa cggcccggtt
    10861 cgtcggccgg atgggctttc cctgcgaaga cgatttggac tggccgccag cggttatgga
    10921 gcaattgttt gagacgttcc aggtcacgaa acaggagggt tgcccgcttg taagtggcaa
    10981 accgtctggc gaacccaatg gtgagagcct ctgggtccaa cagggttcct ctggcgatgg
    11041 cttgcatggg ctggagatgt ccgcgaatcc atccgtcccg cgcgcgctca cgaatgaatc
    11101 gcatgagttt gcgtctggtg gtttgtcgaa ccgcccatag ggcgtcatca ggcaggtctg
    11161 taacccgttg ccagatggtc gggtcgtcga tgcgctccgc ccagtcgggg ctaagatatt
    11221 tggcatagag cgagttcgat tccggagaaa tccatgtcgg cgcgtgaatg ccgttcgtga
    11281 ggctgcggat gggaacaaga tcctgcgcca gaccgggcca cagatgttgc cacatttggc
    11341 gagagacacg gccgtgttcc cggctcacgc catttacgtg ggctgatagc cgcatggcga
    11401 gcgcagtcat attaaagccg tgaccagcgc tttccggcgt ttctccaagc cgcaaaaatt
    11461 cttctctcga tagtcctagc tggccccagt aattgtgaaa gtaccggtcc atcagatgga
    11521 aggggaagat gtcatgtccg gcagggacag gggtatgcgt ggtgaacacg gtgctttgcc
    11581 gaacgagctc actcgcttcg gcatgactgt gtcccgtgct gacgaactct cgaatccgtt
    11641 ccagcgtgag gaaggcggaa tggccctcat tacaatgcca gacggctggg gtgatgccga
    11701 gggctcgcag gacgcggacg ccgccgatgc caagaagaaa ttcctgacac agtctcgtct
    11761 cctgatcgcc gccatagagt ctcgccgaaa gcgcgcgatc ttcaggcgtg ttttcaggca
    11821 catcggtatc aatcaggtag agggacatcc gaccatttcg gacctgccag acaagcacgg
    11881 tgacttgacg atggccgatt tgaactttga tggtgcaagg aacgccggcc ggagtcagcg
    11941 cttggtgaat cggcgaatca tatctattga agggcacata ggtcgcttcc tgccaaccct
    12001 caggattgat tcgttgtttg aaatacccct gcggatacat gaacccgatc cctacgagcg
    12061 gaatgcctaa gtcgctggcc tccttgcaat gatctccggc taagatgccg aggccgccgc
    12121 tgtaaatcgg aattgaaatg tgcagtccga actccgctga aaagtaggcg atcggagaat
    12181 tctggagtgc agggaattcc gtggccgccc acgtgttctt gctgctcatg tattcgtcaa
    12241 agctgcgcag gacggcggag tactgtcgtg cgaaggtcgg atcactggcc agcgtcgcga
    12301 atcgttccgg tttcactcca gcgagtaatt gaacgggatt gtggtgggtc aggaaccaca
    12361 aggttgggtc gatcgactca aagagctgcc gtgccggttg gttccagctc caccacaagt
    12421 tgcgggccag ttcgccaagg cgatgcaggc tttgcggaac catatcctga gggggctgta
    12481 tgtggtcagg tgcttgcacg acgcctccgt ggaaggtgat cgatgggtga ggaaggggcc
    12541 ggtatggtcc cgcttccgga atgctgtgtc aggcgtgtcc ggctttatgc caggaggccc
    12601 attggtcaga aaaggcgacg gtggcgtagc gctctccgag aatcttggcg actcgattca
    12661 tggtggccgt cagttgtttc gcctcctcct gtgtcaagtc ttgacgaaaa cgcgggtgga
    12721 gtccccaacg cgtctcaact tcttcgccct tgatgctcga catgacgctc accagcttaa
    12781 tatcggcccc ggtggcgccc cgagggttgc tgctggcggc actggggttc gcattgcctg
    12841 tggcgtcggc cgggactgct ggcgtcttgc cttcaaagat gccgatcaga gccggaatga
    12901 cagcggcaat gcagagtgtc gccgcggcct caaccttcgg gtttcccgat gccccgaccg
    12961 cacgggcgac acgatcgctg aaatcgcgat acaaatcatt tttggccggg ctctcctctg
    13021 taaccaggaa tcgataccgc tcgtatgtct ggcgactctc ggatacagcg gccccgatcg
    13081 tcatgacgat ttccatctgg tcatcgccgg ctccgaatgc cggttccaat actttcttga
    13141 gctgatcgat cactgtatcc gtcagttcgt ccatgaactg cggttgttcc ctcagtggga
    13201 tatggagcgc cgatacccga tcggtcagat tgaacattgc ccgtaaaaat tcgagataaa
    13261 tgccccattc gtcctggcgc ttcagtttga gggcctgttc cggcgccgcc cgtttgatca
    13321 ccgagacgga ttccccagac acggcaagca tcagttccgc cacggtgcgc atctgttgag
    13381 cgaacgaatg tgatttagtc gtcataatgg ttccctgctg gtcgggcatt atatacaact
    13441 caccttgaag ctccaagtgt tgatctcgtg cgccctgatc atggacggtg gctcgcgcgc
    13501 tgtcgttgca aggcacgggg tggtatgtta tacagcgcga ccgctcccgt catgagcctc
    13561 accgcccaat cggatgccaa catggatcga gacacgaaac cctacattct caaacggact
    13621 ccggatcagg acgtggtccg gaagttctcg ttggattatg ccaaagagct caatccccag
    13681 cagtatgcgg cggtgacggc cgcggatggc ccggcactcg tcattgccgg agcggggagt
    13741 ggaaagacgc gcaccctcgt gcatcgcgtc gcctatttga tcgattccgg tgtcgatccg
    13801 tcccagattc tgttgctgac cttcacccgc aaatccgcag aagaaatgct ggagcgtgtt
    13861 ggcgcgttga tcggctcgcg cagccaacgg gtgtgcggcg gtacgtttca ctcggtggcg
    13921 aacatgctct tgcggcgcta cggtcgagtg ctggggattg agccaagttt taccattatg
    13981 gatcgcgggg atgccgagga tctgattgct ctgttacgcg cccagctcgg actcaatgag
    14041 aaggataagc gtttcccccg caagggcact atcgcggaaa tttacagtaa gtgtgaaaac
    14101 accttgcgcg ggctcgaaga aattgttctt gatgagtttt cgcattttgc cgatcacctt
    14161 gatgcactcg gcaaactgca acgcgcctat cagctggcca agcgacagcg tcaactgctc
    14221 gactatgatg acctgctcgt cttattgcgc gacctgatga cgcaggatga ggcaagccgg
    14281 caggctattt cacggcaatt ccgttacatc ttggtggatg agtatcagga tacgaatcgg
    14341 ctgcaggcgg aactcatccg gaagctggcg gccacgcatg acaacgtcat ggtggtgggg
    14401 gacgattctc aatcgatcta cgcctttcgc ggcgcgacgt tccgcaacat catggaattt
    14461 ccctcctggt ttcccggcgc gaccatttat aagcttgaag aaaactatcg cagcacgcag
    14521 ccgattctca gtctggcgaa tgagattatt caggaggcgc ctgagaaata tacgaaacat
    14581 ctgttcacga ggaagttgga tggaccgttg ccggcgctgg tcgaggctgc cggggaaaat
    14641 gctcagtctc gcttcgtcgc gcaaaagatc ctagagttgc gggaggaggg tgtcccgtta
    14701 aatgaaatcg cggtgttgtt ccgttccagt tttcactcct ttgatttaga aattgagttg
    14761 tcacgctgtg gattgccgtt tgtgaagcga ggcggcatca agttcatcga ggcggcgcat
    14821 gtcaaggatt tgttggccca tttacgcgtg gtggtgaatc cgcatgacgc ggtgagctgg
    14881 catcgtgtgc tcatgctggt cgaaggggtc ggtccgaaaa aagcgcagga tctcgttgca
    14941 gccatggtga gggtgactga tccctatcag gtgttgcggg acagtagcgg gcggtcaggg
    15001 aaagggttga aagagctcgc ggtggtgctc gagaatcttt cgaagagcga cgatttgagc
    15061 ccgaccgagc aggtcaatcg ggtgtatgag tattacctgc cgattctcaa agaccaccat
    15121 gacgactatc ccaagcggat tcgagacctc gatcatttgc atacgattgc cgaaagttac
    15181 cccggcctaa cggaatttct ggcggacttg gctctggctc cgccggatgg cagtgcggtc
    15241 ggagtggaag cggctggttc ggacgacgag caggtggtgc tgtccacgat tcattccgcg
    15301 aaaggcctgg agtggcaatg cgtgtttctg ttgtgggtcg tggacgggaa gtttccgtcg
    15361 gtgttttcgt tcaataccga tgaagaattg gaagaagagc ggcgattgct ctatgtcgcg
    15421 gtcacgcggg cgaagcgcta tctgtttctg acctatccga tcaatgtcta tgacaggagc
    15481 tcgggcatgt tgttgtccaa gccttcccgg tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnntggtggg aggcatgctg ctggtgctgc tatgttctgc gccgattctg
    15661 gctgagactc cggcggccca gcctgatgcc tctcgcctct ggagcggctt ggtggcggaa
    15721 gcgaaagccc tccggctacc cactcaattt cttgagcaag tgccgaagca ttttgtgaca
    15781 ttcgaattcg aggatctgca tgcctttgcc gcggagtatc atccagcgga ccatcgcatg
    15841 gtgctggatc gatcgctgtc gctgaatgcg gcgggccgca cgttgcgacc gttggggcga
    15901 ttgacgcata aggagctgga aacgctgtat cacgagctgt tccatgtcta tatggacttt
    15961 ctcgagcagg aagctacacc gtcggaagcc catgcttcct tgattgcgtt tgcgcgcgag
    16021 caacagcatt gccgatatca acaggtcctg atcacaccgg tgcttcagaa aagggcgctc
    16081 aaggaagagc gatttctgag tgagacggaa tcgtgggaag ttctcaatga aacctgggcg
    16141 gtgtttatcg ggtgggcggt gtggacgcag ttggaagtcg atcaaaaacc gcggcgtgat
    16201 tcccggggac ctgcccatac ctctggctgg atcgcgcggc tcgatcaggc cgagcaggac
    16261 gcggcgttga ttgggtacta cgaacctgag gacctggagg agaaacgtat ggcgcgcaag
    16321 cgatttctgg cgccagagtt tcgcctgtct tcaccggaac tcctgcgcct catgaaggat
    16381 gtattgggga gtcagccgga cgtcattcgt caagccactc aggtgcttca acgttcgcga
    16441 ccagttgcca gtacgcgcgg ggcctgtgcg gcatcaacga agccgtagaa ttgttcctgc
    16501 atagaacata cccccttgac aatagaacgc gtcgcaaata aaatcggctg ctttcgaagc
    16561 cagatcgacc ctcaccttat cagcgatcca gccctgtcgc tttgctggag agctgtgcat
    16621 ggtggggctt acttcattct ctgaaccgga aaatgaaacg ctctggtgct tgttttcttt
    16681 gaattgattt gtcgcgcgct tacgtttgga aataggaccc tcgcgggcag gtacccaagt
    16741 ggacaaaggg ggcagactgt aaatctgctg ccttatg
//
LOCUS       DAOQEW010000074        16763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_913_length_16763_cov_8.334630, whole genome shotgun sequence.
ACCESSION   DAOQEW010000074 DAOQEW010000000
VERSION     DAOQEW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16763
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_16763_cov_8.334630"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLS35_09690"
     CDS             <1..606
                     /locus_tag="PLS35_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI18978.1"
                     /translation="LAAATARIGVAKADRFPKLSITGILGVASPQLSRLIANETAFGV
                     VGPGLAGPLLNAQILGFQQDAAEAQSRKVLARYEQAVLVAFREVEDALVAVRTSREQR
                     DAQAQQVDALRSALRLANLRYKGGLANYLDVLVAQRNLFEAELALTGTHRLQLVSVVQ
                     LYKALGGGWSPLDMAQQQPGQAPGTRGHALPAAGTSPAGHG"
     gene            1031..4255
                     /locus_tag="PLS35_09695"
     CDS             1031..4255
                     /locus_tag="PLS35_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI18979.1"
                     /translation="MPMKPVILFVDANSVHVNALREALHGEEAELLCAASSAEAAEVL
                     LTREVVLVVMAARLLQTEGFKCAETLRGMKRTRHVPLILITEAGDDEQLTIPANDAGI
                     LDSLPTPIDPRQWRRKFRMYLEVSRQRQELSQQRMALRTALDEQRGMQAQLRDEEERY
                     RLALTATRDAVWVWNIETDSQVWNQAGADLFGWSEVVEQPQPAQWWVDRVHPDDRERV
                     GGAFHEAIGTPSVTHWADEYRFLKRDGTYAWVLDHGYIVRDVRGSAKRAVGAMQDITE
                     RKRAQEIAAHFAAVVGSSFDAIVSKTPEGVITSWNAAAERMFGYSASEMIDHTAHRLI
                     PVELHAEENDILARISCGESIQHYETVRLTKDGRRVDLSLTISPIRDSKGQIIGSSQI
                     ARDITEQKRQRTALKESEELFRALVDQMSQLAWMMDDTGNVYWFNQRWYDYTGMTIET
                     MHGEAWDQAHHPDHRARVLSSRRRSLETGEPWEETCLLRGKDGQYRWYLTRAVPIRDD
                     QGRIVRWFGTNTDITEHKRTEEDLRTAEQALRQSDTRFRELVERSPFGIYVVDAEFRI
                     AHMNEAGQKGAFCNVRPVIGRDFGEAMRILWPEAVAADIIKQFRHTLETGTPYRSRDF
                     INPRADLQQTEGYEWELHRLTLPDGRHGVICYYYDSTRLRMVEVALRESGERLQAAAA
                     DLEKRVDERTRDLVRSQADLRALASELNLTEQRERKRLAGELHDHLQQMLVFGKLQLG
                     RGMMQTGVDPECMEVIRDVYGMLSEALTYTHTLVAELCPPVLREHGLLAGLRWLADYM
                     KRYGMKVTVKLPDEEDLALPEDCTILLFQSVRELLMNAHKYAGTGLAEVALVHGQGAL
                     MIHVRDRGKGFDSAAATSADLAVGGSSSKFGLFSIKERMRTLGGSFAIASAPGQGTTA
                     TLRLPLTSRAAGAERVGDEKEVPAGTPAVQPNPMGTRPIRVLLVDDHVMIRQGLRAVL
                     EGYSDIELIGEAGNGEEAVALVDRLEPSVVVMDINMPKMNGIEASGRITMRHPAVAVI
                     GLSVNASADNQSAMLKAGARQLLTKEAAAEQLYGAIKSAVGISA"
     gene            complement(4294..5160)
                     /locus_tag="PLS35_09700"
     CDS             complement(4294..5160)
                     /locus_tag="PLS35_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI18980.1"
                     /translation="MKNLLFPIMMLSLSLAASGCSQATFTAVNLPTRFHEMTVVQDVA
                     YSPEPACTLDIYVPASAGHQPLDVIVFFYGGRWTEGEKHNYRFVGAAFTEQNFIVVVP
                     DYRKYPRVRFPEFVEDGAKALAWVYDHIAGYHGNPERIHVAGHSSGAHIGALLTADAG
                     YLANEGKDRSRVIRDFAGLAGPYAFTPDEPDLEDMFGPPSNYPNMQVTTFIDGRQPPM
                     LLLYGDADRAVKVANLERLEQRIKEKGGRVETQIYPGAGHRDLAAALTWLNLRRTPVL
                     RDMQRFFRSSVR"
     gene            5457..5855
                     /locus_tag="PLS35_09705"
     CDS             5457..5855
                     /locus_tag="PLS35_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNI18981.1"
                     /translation="MTKLLRITTKQLKEIVDVTELLQATIRNANMKEGLCSVFVTHTT
                     AAVTTGEIGEGTEQDFLQIVEEVIPRIQFRHAHDPSHAWSHMAASMLGPSLTIPVSGG
                     TLVLGTWQSVMLVELDGPRDRNVHVSLMSF"
     gene            5917..6459
                     /locus_tag="PLS35_09710"
     CDS             5917..6459
                     /locus_tag="PLS35_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI18982.1"
                     /translation="MAEIPAGGVIADFLAEDHRRLDALLQATDAHPGRIDREAYDQFR
                     AGLLRHIGMEEKLLLPALQRWRGGEPLPVAARLRLDHGALATLLMPTPTAQILATIRR
                     ILSEHNRIEEGPDGLYALCGQLPDTEATRLWDALRAAPTVSVMRHSDSPAVMKTLEGA
                     LARAGYRLEALAPREEGESR"
     gene            6482..7582
                     /locus_tag="PLS35_09715"
     CDS             6482..7582
                     /locus_tag="PLS35_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18983.1"
                     /translation="MMIRHPRRLPLLALGMLALLTGLWAGVARLGWDVPLPRSGFSSL
                     HGPLMVSGFLGTLISLERAVALGRSWAYAAPLLTGLGALVLIAGGPLVVAQLLLLSGS
                     AGLAAIFAAIIRRHPALYTFTMGGGAIVWAVGNLLWVLGWPVFLVVFWWAAFLVVTIA
                     GERLELARLQQVTRGAQTAFLLFLGVLGAGLLLLGWWFDGAVRLFGAGLAALALWLGL
                     NDIARRTVHQTGLTRFIAICLLSGYAWLGVSGLAAMVYGGVPVGPQYDAMLHAFFLGF
                     VFAMIFAHAPIIFPAVLGARMTYRPVFYAHVLLLQATLMVRLVGDAAGWGAGRQIGGL
                     LNAVTLVLFLANTVTALGSLPDRPVVTSRAGS"
     gene            7632..7955
                     /locus_tag="PLS35_09720"
     CDS             7632..7955
                     /locus_tag="PLS35_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNI18984.1"
                     /translation="MTREKQGAQSDPRVMEALRQVVDPELGINIVDLGLVYGSEVREG
                     QVHVTMTMTTPACPMEELLMEMVHSAILRELSEARSVEVDLVWDPPWKPDMMSPAAKA
                     QLGRT"
     gene            8015..9265
                     /locus_tag="PLS35_09725"
     CDS             8015..9265
                     /locus_tag="PLS35_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNI18985.1"
                     /translation="MTENQSQQVRALVDEVYRTQSRQVLATLIRLLGDFDAAEEALHE
                     AFAVAVEQWAREGVPANPRAWLVSTGRFKAIDGMRRRARFDASLPDLAHHLELTADPV
                     IDEGEDSVEDDRLRLIFTCCHPALSPEAQVSMTLREVCGLTTDEIARAFLTKPTTIAQ
                     RIVRAKGKIRDARIPYEVPSETDFPDRLDAVLRVVYLVFNEGYSASSGDSLTRHDLSG
                     EAIRLGRVLVGLLPEAEVLGLLAVMLLQDSRRAARTSPTGDLILLEQQDRSLWNRDQI
                     AEGMALVELALATRRVGPYTIQAAIAAVHAEAPEAALTDWAQIVGLYDVLLQAEASPV
                     VELNRAVAVAMRDGPSAGLALVDAILSRGDLTNYHLAHSARADLCRRLGRNTDAAASY
                     ERALALTQQEPERRFLEGRLRELG"
     gene            9404..9817
                     /locus_tag="PLS35_09730"
     CDS             9404..9817
                     /locus_tag="PLS35_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI18986.1"
                     /translation="MAKRTPTRKTTKKADCPASLVWFDIPADDLARAKKFYSALFGWK
                     ISRFPAMPDYWHIDTGGEKATLDGGLLPRQAPRQPITAYVSVPSVEKAAAKVQKLGGT
                     VCKTKTPVPGMGYFVICQDTESNTFALWEMNPQAT"
     gene            9872..10228
                     /locus_tag="PLS35_09735"
     CDS             9872..10228
                     /locus_tag="PLS35_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNI18987.1"
                     /translation="MKYLCLVYVEEKILHALPSAQRQTLSDESMSYCDRLQKLGQLVT
                     ASPLHPVETATTVRVRDGKTSTIDGPFAETKEQLGGFLLLDVPDLNDAIRIASQFPAA
                     RIGSVEVRPMKEQGCA"
     gene            10316..11497
                     /locus_tag="PLS35_09740"
     CDS             10316..11497
                     /locus_tag="PLS35_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HNI18988.1"
                     /translation="MSRVRVLVGTRKGAFILTSDGTRKQWDVTGPHFGGWELYHLKGS
                     SADPNRLYASQTSSWFGQVIQRSDDGGKTWNPPGTKPEDLMGADGMPNGASNMFVYDQ
                     SAETGKPLTTHQHYDGTQRPWEFKRVWHLEPSLTDPDTVYAGVEDAALFRSTDGGRTW
                     QELAGLRDAKGKLWQPGAGGMCLHTIVQDPANPQRMHIAISAAGVFRTDDGGKSWRPT
                     NRGLKSQFELPDPDAEVGHCVHCITMHPSRPNVLFMQKHWDVMRSDDGGESWHEISGN
                     LPTDFGFPIAVHAHEPNTVYVVPIKSDSEHYPPEGKLRVYRSRSGGNEWEPLTKGLPQ
                     QNCYVNILRDAMSVDSLDPCGLYFGTTGGQVYASADGGDSWAPIVRDLPAVLSVEAQT
                     L"
     gene            11693..11989
                     /locus_tag="PLS35_09745"
     CDS             11693..11989
                     /locus_tag="PLS35_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNI18989.1"
                     /translation="MVRVILPQHLRTLAKVEGEVSLEVVGAVTQETVLDALEVRYPVL
                     RGTIRDHVTKRRRPFIRFFACEQDVSHDSPDVPLPESVAKGAEPLLIVGAMAGG"
     gene            12025..12387
                     /locus_tag="PLS35_09750"
     CDS             12025..12387
                     /locus_tag="PLS35_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNI18990.1"
                     /translation="MKYLLLVHHSEEAFAKMAETTRREMLAESVQLTHQLHAKGQYVS
                     ASPLHPTSTAARVQVREGRAIVTDGPFAETREQTAGYFLVEASGLQDAIDIAARIPGA
                     RLGTVEVRQVREIDGLPE"
     gene            12597..13064
                     /locus_tag="PLS35_09755"
     CDS             12597..13064
                     /locus_tag="PLS35_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI18991.1"
                     /translation="MSTSHAKPIPDGMHTITPHLICAGAADAIEFYKKAFHAVELGRV
                     PGADGKLLHALIRIGDSPVMLVDEFPDHHTLGPKSIKGSPVTIHLYVKDVDTVFNRAV
                     SAGAKSTMPVSDMFWGDRYGQLEDPFGHRWSVATHVRDVDPQALQEAARKACE"
     gene            13107..13478
                     /locus_tag="PLS35_09760"
     CDS             13107..13478
                     /locus_tag="PLS35_09760"
                     /inference="COORDINATES: protein motif:HMM:NF018893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HNI18992.1"
                     /translation="MRYVDGFVLAVPKKNVNAYFKMAKKACKVWTEHGALEYRECVGD
                     DLAVKMGLPFPRLAKLKQGETVVFSWIVYKSRSHRDRVNAKVMKDPRINEMCDEKAMP
                     FDVKRMAYGGFKVMVDGGTRS"
     gene            13672..14097
                     /locus_tag="PLS35_09765"
     CDS             13672..14097
                     /locus_tag="PLS35_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNI18993.1"
                     /translation="MRFMIIIKATKDSEAGVMPSQQLLSEMGTFNEELVQAGVMLAGD
                     GLHPSTKGARVRFSGTRRTVIDGPFAETKELIAGYWLWQCGSKEEAIEWVKRCPNPMP
                     GESEIEIRQVFEAEDFGAEFTPELREQEDRLRAQMAQNH"
     gene            14267..14866
                     /locus_tag="PLS35_09770"
     CDS             14267..14866
                     /locus_tag="PLS35_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNI18994.1"
                     /translation="MRVTSILLACLCTSLMTMPVSAKEKKSEKAMDPQAMMEVWKKAA
                     MPGEPHKLFASLAGSWTTQTKEWMEPGKPPSETTGTAEMKMLLDGRFLYQEFNGTMMG
                     QPFAGIGIDAYDNMTKKYVTAWMDSMGTGIFTMQGTSSPDGKTITLKGSHPEPGGGQM
                     HHRAVWKIIDQNSQTFEMYGTHGHGKEMKFMEITYTRKS"
     gene            15254..16450
                     /locus_tag="PLS35_09775"
     CDS             15254..16450
                     /locus_tag="PLS35_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI18995.1"
                     /translation="MIRRWRWSTIAPMVCGWLALALVPGCKQEAGSAPAPTVPEVGIV
                     VATAQDVPDEPEFIGQAESSRPVEIRSQVTGILKQWYFKEGRDVKKGDRLYQIDPVPF
                     HAAMLSAKAKVAQSEARLVQANQNLTRVKPLLAEQAVSTKDVDDAVAEQLAAKAALEG
                     AKAELVKAKFDLDNTLIIAPIDGMIERTRVYEGRLVSAQTDLLTMIHQVDPMYVIVSA
                     PESFLLKRRRDTDAKRIQHPGVYQLRGVLTFVDGTTYGHEGVLDLLDVGLKTDTGSRQ
                     ARVVFPNPDRVLLPGQFVRVRFKGTLKTGAILVPQRAVQQGAKGSIVFVVGKDDKVEM
                     REILASSWQGSQWIVEEGLHVGDRIIVEGLHKIAPGAVVKPVPVPAAGAATAPSAAPA
                     PPEPAS"
     gene            16447..>16763
                     /locus_tag="PLS35_09780"
     CDS             16447..>16763
                     /locus_tag="PLS35_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI18996.1"
                     /translation="MISHFFIDRPIFASVLSIVIMVLGLVALQALPIAQFPEITPPVV
                     QIEADYPGANAEILADSVARPIEVQLPGIDNLLYYDSTSTNDGHMSIKLTFEIGTDVD
                     IAQV"
BASE COUNT         3319 a         4628 c         5428 g         3388 t
ORIGIN      
        1 ttggcggcgg ccacggcgag gatcggggtc gccaaggcgg atcgattccc gaagctgagt
       61 attaccggga tcctgggcgt ggcgagcccg caattgtcgc ggctgatcgc caacgagacg
      121 gcattcgggg tggtgggccc cggcctggca ggaccgttgc tgaacgcgca gattctgggc
      181 tttcagcagg acgccgccga ggctcagagc cggaaagtgc tggcccggta tgaacaggcg
      241 gtgctggtcg cgtttcgcga agtagaggat gcgctggtgg cggtgcgcac gtcgcgggaa
      301 caacgggacg cgcaggcaca gcaggtcgat gccttacggt cggccttgcg gctggccaac
      361 cttcgttata agggagggct ggccaattat ctcgatgtgc tggtcgcgca acgcaatttg
      421 ttcgaggcgg agctggcatt gaccggtacc caccggctgc aactggtctc ggtggtgcag
      481 ctctacaagg ctctcggtgg gggatggtcg ccgctggata tggcgcaaca gcaaccgggg
      541 caggctccag gaacgcgagg gcacgctctc ccggccgccg gcacctcacc ggccggtcac
      601 ggatgatggc gtcggccggc tcgctcacct tctgccagag tgtcacctct gcctgattca
      661 gtctggtggt cgtcgctttg tcatagcact gctccgtctg attctccctc tgtacggtga
      721 ccgcggggcg gaccgcagga aattcgcttc catatttcag ccggtgtata ctccatcctc
      781 ccctttgcct accgaacttt tcttaggttc gaccaaggag taggcgtttt ctgagtccag
      841 attgagcaca gtcgaccagg ggctatgccc ttgatgtgga acggcgaatt gcgtggttct
      901 gcagagacgc cgttttccac aaccaccatc cgggtgctac atgccgggat gtctcgatag
      961 agggggccat cgcgcgggta atggcacagg ctccccaacg agagatgaca ctcaggagga
     1021 cgtacccgct atgccgatga agcccgtcat tctgttcgtg gatgcgaaca gcgttcatgt
     1081 gaacgcgtta cgcgaggcat tgcacggcga agaggccgaa ctattgtgtg ccgcctcgtc
     1141 tgctgaggca gccgaagtgc tgctgacacg tgaggtggtg ctcgtggtga tggccgcgcg
     1201 gctgttacag acggagggat tcaaatgtgc ggaaacgctg cgtggaatga aacgtacgcg
     1261 ccatgtgccg ctcatcctca tcacggaagc cggtgacgat gaacagctga cgattcccgc
     1321 gaatgacgcg ggaatactgg attccctgcc tacgccgatc gatccgcgtc agtggcgcag
     1381 aaagttcagg atgtatctcg aagtcagccg gcagcggcaa gaattgtctc agcaacgcat
     1441 ggctttgcgc acggctctcg atgagcagcg agggatgcaa gcgcagttgc gggatgagga
     1501 ggaacggtac cggctcgcat taaccgcgac gcgggatgcc gtctgggtgt ggaacattga
     1561 aaccgacagt caggtgtgga atcaggccgg cgctgatttg ttcggttgga gtgaggtcgt
     1621 cgaacagcca caacctgcgc aatggtgggt agaccgggtt catccggacg accgtgaacg
     1681 tgtgggtggg gcgtttcacg aggccatcgg caccccgtcg gtgacgcatt gggcggatga
     1741 gtatcgcttc ctgaagcggg atggaaccta tgcgtgggtc ctggatcatg ggtatatcgt
     1801 ccgcgacgtg cgcggctcgg cgaagcgtgc ggtcggcgcc atgcaggata ttaccgagcg
     1861 taagcgggcc caggaaattg cggcgcactt cgcggctgtg gttggctcgt cgttcgatgc
     1921 gattgtcagc aagacgccgg aaggcgtgat cacgagctgg aacgccgcgg ctgaacggat
     1981 gttcgggtac tccgcatcgg aaatgatcga tcacacggcg caccggctca ttccggtcga
     2041 gctccatgcc gaggagaacg acatcctcgc gcgcatttca tgcggggaat ccatccagca
     2101 ctacgaaacg gtcaggctga cgaaagacgg ccggcgggta gacctttctt tgacgatttc
     2161 ccccatcaga gattccaagg ggcagatcat cggatcctct cagatcgcgc gggacatcac
     2221 ggaacagaaa cgccagcgta ccgcgctcaa ggaaagcgaa gaactgtttc gcgccctggt
     2281 cgaccagatg tcgcagctgg cctggatgat ggacgacacc ggtaacgtct actggttcaa
     2341 tcagcgatgg tatgactata cgggcatgac gatcgagacg atgcacggtg aggcctggga
     2401 ccaggcccac catcctgacc atcgtgcacg cgtgctctcc agcaggcgcc ggtctcttga
     2461 gacaggagag ccgtgggagg agacctgcct gctgcgcgga aaggatggcc aatatcggtg
     2521 gtatctcacc cgtgccgtcc cgattcgcga cgatcaaggc cgcatcgtcc ggtggttcgg
     2581 tacgaacacc gacatcaccg aacacaaacg aaccgaggag gacctgcgaa cggcggagca
     2641 ggctctgcgc caaagcgaca cgcgattccg tgaattggtg gagcggtcac cgttcggcat
     2701 ctacgtcgtc gatgccgagt tccgcattgc gcatatgaac gaagccgggc aaaagggcgc
     2761 cttctgtaac gtacggcctg tcatcggccg cgacttcggc gaggcgatgc gcattttgtg
     2821 gcccgaggct gtggcggctg acattatcaa acagttccgt cacacgttgg agacgggcac
     2881 cccgtatcgt tcgcgcgact tcatcaatcc gcgcgccgac ctccagcaaa ccgaaggcta
     2941 cgaatgggaa ctgcatcggc tcaccctgcc tgatggccgg cacggagtaa tttgctacta
     3001 ctatgattcg acgagactcc ggatggtgga agtcgccttg cgcgagagcg gggagcggtt
     3061 gcaagcggct gcggccgacc tggagaagcg ggttgacgaa cgaacgcgtg atctcgtgcg
     3121 gtcgcaggcg gacctccgtg cgctggcgtc agaattgaac ctgaccgagc agcgtgagcg
     3181 caagcgcctg gccggcgagc tgcacgacca tctgcaacag atgctggtct tcgggaaatt
     3241 acagctgggg cgcgggatga tgcaaaccgg ggtggatccg gagtgcatgg aggtgatccg
     3301 cgacgtctac ggcatgctct ccgaggcgtt gacctacacc catacgctgg tcgcagaatt
     3361 gtgtccgccg gtattacgcg agcatggact gttagcaggg ttgcggtggc tggccgacta
     3421 tatgaagcgg tatggcatga aggtgacggt gaaactgccg gacgaggaag atttagcgct
     3481 cccggaagac tgcacgatct tgctgtttca atccgttcgt gaattattga tgaacgcgca
     3541 taagtatgcc gggacagggc tggcggaagt ggcgctcgtg catggacagg gcgcgctcat
     3601 gattcacgtg cgggatcgag gaaaaggatt tgatagtgct gcggccacct ccgcagatct
     3661 ggcggtcggc ggcagctcat caaagttcgg actgttcagt atcaaagaac gcatgcggac
     3721 attgggcgga agttttgcga tcgcctccgc gcccggtcag gggaccacgg cgacactgag
     3781 gctgccgctg acatctcggg cggcgggggc ggagcgcgtg ggcgatgaga aggaggttcc
     3841 cgctggtacg ccggccgtac aaccgaaccc gatggggacg agacccattc gcgtcttgct
     3901 cgttgatgac catgtcatga ttcgacaggg gttgcgggcc gtattggaag gatactcgga
     3961 catcgagctg atcggggaag cgggaaacgg cgaggaggct gttgcgctag tcgatcggct
     4021 tgagccgtcg gtcgttgtga tggatatcaa catgccgaag atgaatggca tcgaagcctc
     4081 cggacggatt actatgcggc accccgccgt cgccgtcatc ggactctctg tcaatgcgag
     4141 cgcggacaac cagtctgcca tgttaaaggc cggcgctcgc cagctgctga cgaaggaagc
     4201 ggctgcagaa cagttgtatg gcgcgatcaa aagcgccgtc ggaatatccg cctgagtctt
     4261 ctccttgctg cgtcgcatgg cgtctgccgt ctgtcatcgt acgctcgacc gaaaaaacct
     4321 ctgcatgtcg cgaaggaccg gcgtcctgcg aaggttcagc catgtcaggg ccgcggccag
     4381 gtctctatgt ccggctcccg ggtagatctg tgtttccacg cggccgcctt tttccttgat
     4441 gcgctgctcc agccgttcga gattagcgac ctttaccgcg cggtctgcat cgccgtataa
     4501 cagcaacatc ggcggttgcc ggccgtcaat gaacgtcgtc acctgcatgt tcggatagtt
     4561 gctcggaggg ccgaacatgt cttccaggtc cggctcatcc ggagtaaagg cgtagggccc
     4621 cgcaagcccg gcaaagtcac gaatcacccg tgaccggtcc ttgccctcat tggccagata
     4681 tccggcatcc gccgtgagca aggcgccgat gtgggctccg gaagaatgtc ccgcaacatg
     4741 gatccgctcc gggtttccgt gatatccggc aatgtggtcg tacacccagg ccagcgcttt
     4801 cgcaccatcc tcgacaaact ccggaaagcg gacccgggga tatttgcgat agtcggggac
     4861 caccacgata aaattctgtt ctgtaaacgc tgccccgacg aatcgataat tgtgcttctc
     4921 tccctccgtc catcgtccgc catagaaaaa tacgatcaca tccaacggtt gatgcccggc
     4981 gctggcagga acatagatgt ctaatgtgca cgcaggctct ggggagtagg ccacgtcctg
     5041 caccaccgtc atctcatgaa agcgagtggg aagattgacg gccgtgaagg tggcttgcga
     5101 gcagccggat gcggccagag acaagctcag catcattatg ggaaacagga gattcttcat
     5161 gccgtgcctt gcgacctcat acgccccagg ggcgggcgga gaagaccccg cctgcgcctc
     5221 ggcctaaggg gccatgtgac tggcggtccg gtactcatac cacaacgaag atgtccaagt
     5281 tcatgtccac gcaacttccg ggagcgtatg gccttgacat accgcaccct ccgtggcagg
     5341 aacgatgcct cgcacagcgc caaccatcgt tgcacgggtc gtgcatccgc tgtagtgtga
     5401 gatgtatgaa cctgtcctaa tgaggaggct cccatgggac gccaggcgag ggctctatga
     5461 cgaaactcct gcgtatcacg accaaacagt tgaaggagat agtggatgtg accgagttgc
     5521 tccaggcaac cattcgtaac gccaacatga aggaagggct gtgctcggtc ttcgtgaccc
     5581 acacgaccgc ggctgtgacg acgggcgaaa tcggggaggg gaccgaacag gattttctcc
     5641 agatcgtcga agaagtcatc ccgcgcattc agttccggca tgctcacgat ccgtcccatg
     5701 cctggtcgca catggctgcg tcgatgttgg gtccctcctt gaccatcccg gtatcagggg
     5761 ggacactcgt actcggcacg tggcaatcgg tgatgttggt ggaacttgat ggcccgcgtg
     5821 atcggaacgt gcacgtctcc ctgatgtcat tctgacagac gtctgaaaga tgcgaggcgg
     5881 ccgctcatga gccgcgctgg ttcagaggga ggcgagatgg cagagatccc ggccggcggt
     5941 gtgatcgccg attttcttgc ggaggaccac cggcggctcg atgccttgct gcaggcgacc
     6001 gatgcgcacc ccggccggat cgaccgcgag gcctacgatc agttccgtgc gggcctgctg
     6061 aggcatatcg gcatggagga aaaactgttg ttgcctgcat tgcaacgatg gcgaggcggg
     6121 gagcccttac cggtggctgc gaggctgcgg ctcgaccatg gcgcgctggc cacgttgctc
     6181 atgcccaccc cgaccgctca gatcctggcg acgattcgac ggatcttgtc ggagcataat
     6241 cgaatcgaag aaggtcctga tggattgtat gccctctgtg gtcagttgcc ggatacagaa
     6301 gcgacacgac tgtgggacgc attgcgcgct gcgccgacgg tgagcgtcat gcggcattcg
     6361 gacagtccgg cggtcatgaa aaccttggag ggcgcacttg ccagggccgg atatcgtctc
     6421 gaggcgctcg ctccgcgtga ggagggagaa tctcgatgac cgtgccgaag ggaccgtcac
     6481 gatgatgatc cgtcatccgc gacggcttcc actgcttgcg ctcggcatgt tggccttact
     6541 gacggggctc tgggccggcg tcgcgcgact cgggtgggat gtgcctctgc cgcgatccgg
     6601 gttttcctca ttgcatggtc cgttgatggt gtccggcttt cttggaacgc tcatcagtct
     6661 ggagcgagcc gtggcgctcg gccgatcctg ggcttatgca gcgccgttgc tcaccggtct
     6721 tggcgccctg gtcttgatcg caggcggtcc gctggtggtt gcccagctct tgctgttgag
     6781 tggcagtgcg gggctggcgg cgatttttgc agccatcatc cggcggcatc cggccttgta
     6841 caccttcacg atgggaggcg gggcaatcgt ctgggctgtc ggtaatctgt tgtgggttct
     6901 cggttggccc gtctttctgg tcgtcttctg gtgggccgcc ttcctcgtgg tgaccattgc
     6961 gggtgagcgt ctggaattgg cccgtttgca gcaggtgacg cgcggcgcgc agacggcgtt
     7021 cttgctgttc ctcggggtgt taggtgcagg gctgttgctt ctgggatggt ggttcgacgg
     7081 cgcggtgcgt ctcttcggtg ccgggttggc cgctctggcc ctatggttgg gcctcaatga
     7141 cattgccaga cgaacggtcc accaaacagg gctgacacgc ttcatcgcca tctgcctgct
     7201 cagtggctat gcgtggcttg gcgtgagcgg cctggcggcg atggtctatg gcggtgttcc
     7261 cgtggggccg cagtacgatg ccatgctgca cgcattcttt ctggggttcg tgtttgcgat
     7321 gatcttcgcc cacgcgccga tcatctttcc ggcggtgctc ggtgcgcgga tgacgtatcg
     7381 accggtgttt tacgcgcatg tgttgttgtt gcaagcgaca ctgatggtgc gactggttgg
     7441 tgacgcagcg gggtgggggg cgggacggca gatcggcggg ctcctgaatg cggtgacgtt
     7501 ggtgctgttc cttgcgaata ccgttacggc ccttggcagt ttgccggatc ggccggtcgt
     7561 cacgagtcgc gccgggtcct gatgttgatc atagtgaaga cgtcctcgta gcacgcacaa
     7621 gggagtgttg gatgaccaga gagaaacagg gggcgcagtc agatccccgg gtgatggagg
     7681 ccttgcgtca ggtggtggat cctgagttgg ggatcaacat cgtcgatttg ggattggtct
     7741 atggcagcga agtgcgcgaa ggccaggtcc atgtcacgat gacgatgacg acgccggctt
     7801 gcccgatgga agagctgctg atggagatgg tgcattcggc aatcttgcgc gagctgtccg
     7861 aggcacgttc cgtggaggtt gatctggtat gggatccgcc gtggaagccg gacatgatga
     7921 gtccggcggc gaaagcccaa ctcggccgga cgtgagggtg agcccatgag tgcgggaggc
     7981 ggcaaggtct acccgctcga gaaggattgt gaccgtgacc gaaaaccagt ctcagcaggt
     8041 gcgcgcactg gtggatgagg tctaccgcac ccagtcccgc caggtcctcg ccaccttgat
     8101 tcgcttgctc ggagatttcg atgcggccga agaggcgttg catgaagcct ttgccgtcgc
     8161 agtggaacaa tgggcgcgtg aaggggtgcc ggccaatcct cgcgcctggc tcgtctcgac
     8221 ggggcgcttc aaggcgatcg acggcatgcg gcggcgggcc agattcgatg cgtcgctgcc
     8281 tgaccttgcc caccatctgg aactcaccgc tgaccctgtc atagatgagg gggaggattc
     8341 ggttgaggat gaccggttac gattaatctt tacctgctgc catcccgccc tgtcgccgga
     8401 agcccaggtc tccatgacgc tccgggaggt ctgtggtctc acgaccgatg agattgcccg
     8461 cgcgttcctg acgaagccca ccacgattgc gcaacgcatt gtgcgcgcca aaggcaagat
     8521 ccgcgacgcc cgtatcccct atgaggtgcc gtcggagact gattttccgg accggttgga
     8581 tgcggtgctg agagtcgtct acctcgtgtt caacgagggc tacagtgcgt cgtccgggga
     8641 ttcgctgacc aggcatgatt tatccggtga agcgatacgc cttgggcggg tgctggtcgg
     8701 gctgcttccg gaagccgagg tgttgggact cctggcggtg atgcttctgc aagactcccg
     8761 tcgcgccgca cggacgtcgc cgaccggaga tttgattctc ctcgaacaac aggaccgctc
     8821 gctgtggaat cgagaccaga tcgccgaagg catggccctg gtcgaactgg cgctggcgac
     8881 ccgccgggtc ggtccctata cgattcaggc ggccatcgcc gccgtgcatg cggaagcgcc
     8941 ggaggctgcg ctgaccgact gggcgcagat cgtaggcctg tatgacgtgt tgttgcaggc
     9001 cgaggcgtca ccggtagtgg aactgaaccg ggcggtggct gtggcgatgc gtgacggtcc
     9061 gtcggcgggt cttgcgctgg tcgatgccat tctctcgcgt ggcgatctta ccaattatca
     9121 tctggcgcat tccgcgcggg cggacctctg ccggcgactt ggacgaaaca cggacgccgc
     9181 agcttcatat gaacgggcgc tggcactcac gcagcaggaa ccggagcggc ggtttctgga
     9241 ggggcgattg cgggaacttg gttgaggcga ctggtgtcga ccgccctctg tgttctttaa
     9301 gtggttgata tcgcgacagc ttcatggctg cgtcgaagga tgtcgatttt ccccgccctc
     9361 gacgactatc tcatgacagg agccattcat aagggaggat gccatggcga agcgtacccc
     9421 gacgcgcaag acgacgaaga aagctgattg tccggccagt ttggtgtggt tcgatattcc
     9481 tgccgacgac ctggcgcgag ccaagaagtt ctacagtgca ctgttcgggt ggaagatcag
     9541 ccggttcccc gcgatgccgg actattggca tattgatacc ggcggcgaga aggcgacgct
     9601 ggatggcggg ctgctcccgc gccaggcgcc gcgtcagccg attaccgcct atgtctccgt
     9661 tccgtccgtt gagaaggccg ctgccaaagt gcagaaactc ggagggacgg tctgcaaaac
     9721 caaaacgccc gtgccgggca tgggctattt tgtgatctgt caggacacgg agagcaacac
     9781 atttgccttg tgggagatga atcctcaggc gacctagact gaggggcacg cggcatgatc
     9841 cgcgaacgtt gaatcatacg aggaatagat catgaaatat ctctgtctgg tgtatgtcga
     9901 agaaaagatc ctgcatgcgc tgccgagtgc tcagcgccag accctttcgg acgaatccat
     9961 gtcctattgc gaccggttac agaagcttgg tcagctggtg acggcgtccc cgctccatcc
    10021 ggtcgaaacg gctaccacgg tgagggttcg cgatggcaag acgtcgacga tcgacggacc
    10081 gtttgccgag accaaagaac aactcggcgg cttcctcctg ctcgatgtgc cggatctgaa
    10141 cgatgcgatc cgtattgctt cgcagtttcc tgccgctcgc atcgggagtg tggaggttcg
    10201 tcccatgaag gaacagggtt gcgcgtgacc ggtatcgata gggtgtggtc cccctcatgc
    10261 agtcacgaca tcacctcgcg ccgcaggccg gtgcgaatcg aggaaggagc agcatatgag
    10321 cagggtgcga gtgcttgtgg gaacgaggaa aggggcgttc attctgacat cggatggcac
    10381 ccggaagcag tgggacgtga caggtccgca tttcggcggg tgggaactgt atcacctcaa
    10441 gggatcatcc gctgatccta accgcctgta tgcgtcgcaa accagcagtt ggttcgggca
    10501 ggtcattcag cggtcggatg acggcggtaa gacctggaat cctcccggca cgaagcctga
    10561 ggatctcatg ggcgcagacg gtatgccgaa tggcgcgagc aacatgtttg tctatgatca
    10621 gtcggcggaa accgggaagc cgctcaccac gcatcaacac tacgacggca cgcaacgtcc
    10681 ttgggagttc aagcgagtct ggcacctgga accgtccctg actgatccgg atacggtgta
    10741 tgccggggtc gaggatgcgg cgctctttcg ttcgacggat ggcggacgga cctggcagga
    10801 gcttgccggt ctgcgtgatg ccaagggcaa gctgtggcag ccgggcgccg gcgggatgtg
    10861 tttgcatacg atcgtgcagg acccggcgaa cccgcaacgg atgcacattg ccatcagcgc
    10921 tgccggcgtg ttccggaccg acgatggcgg caagagctgg cggccgacca atcgcggact
    10981 gaagtcgcag tttgaattgc cggatcccga tgcggaggtc ggccactgcg tgcattgcat
    11041 caccatgcat ccctcacgtc cgaatgtctt gttcatgcag aaacattggg atgtgatgcg
    11101 cagcgacgat ggcggagagt cctggcacga aatcagcggg aacctgccca cggacttcgg
    11161 ctttccgatt gcggtgcacg cgcatgaacc aaataccgtg tacgtcgtgc cgatcaagag
    11221 cgactccgag cattaccccc cagagggcaa gctgcgggtc tatcgtagcc gcagcggcgg
    11281 caatgaatgg gagcctctca ccaaggggct gccgcagcag aattgttacg tgaacatctt
    11341 gcgcgatgcg atgtccgtcg attcgctcga tccctgcggc ctctacttcg ggacgaccgg
    11401 gggccaggtg tacgcctcgg cggacggcgg ggatagctgg gcgccgattg tgcgggattt
    11461 gccggcggtg ttgtccgtcg aagcccaaac cctataacga agcgctgaaa ccgccctccc
    11521 gcggcgttct cgcctcggac gcgtcctcaa cgtagcccag ggctacgcct ccggcgcctc
    11581 cgtcggctgc ggccttgctg gcggagcgtt ttgagcgctc gttgggctgg ccggcttttt
    11641 aaggagcctg ttcggacacc agatgtacgt acgttctgtc tggaggtgct tcatggtgcg
    11701 cgtcatattg cctcaacatt tgcggacgct ggcaaaggtc gaaggtgaag tatctctgga
    11761 ggtagtgggg gccgtgaccc aggagacggt actcgatgcg ctggaggtgc gttatcccgt
    11821 gttgcgtgga acgattcgag accatgtgac caaacggcgt cggccgttca tccggttctt
    11881 tgcctgcgag caggatgtgt cacacgactc gccggatgtg cctttgcccg aatcggtcgc
    11941 gaagggcgcg gaaccgttgt tgatcgtggg ggccatggcc ggcggctaat cccgctttgt
    12001 agagagatgc gtggaggcag gtccatgaaa tatctgttgc tggtgcatca tagtgaagaa
    12061 gcgttcgcca agatggctga aaccacgagg cgggagatgt tggccgaatc ggtgcagttg
    12121 acgcatcaac tccacgccaa ggggcaatat gtcagcgcct cgccattgca tccgacttcc
    12181 acggcggcgc gtgttcaggt tcgggaggga cgggcgatcg tgaccgacgg tccgtttgcg
    12241 gaaacacgtg aacagacggc cgggtatttt cttgttgagg ccagtggtct tcaggacgcc
    12301 attgatattg cagcgcgcat tcccggtgcg cgtctcggta ccgtggaggt gcggcaggtc
    12361 cgcgagatcg acggtttgcc ggaatgattt gccggaatga gatgtggcgc gagacaccca
    12421 acgacggttc gaagctgggg tgatcccgac tacacagatg taaccgacat gggcaaggtc
    12481 aatcgcgtga agaggccgat gatggcggaa tgctttcttc aattctcggg ctacacgcga
    12541 agctcgacgg actgttccct caaaccatgg gaacggttca atcgaaagga gcaacgatga
    12601 gcacgtcaca cgccaagccg ataccagacg gaatgcatac gattacccct cacctgatat
    12661 gcgccggtgc tgccgacgct attgagttct ataagaaggc tttccatgcg gtggagttgg
    12721 ggcgggtgcc cggagcggat ggaaagcttc tccacgcgct gattcgcatc ggggattctc
    12781 ccgtcatgct ggtcgatgaa tttcccgatc atcacacgct cgggccgaaa tcgatcaagg
    12841 ggtcgccggt caccattcat ctgtatgtca aagatgtgga tacggtcttc aaccgcgccg
    12901 tttcggcagg cgcaaaaagc acgatgccgg tcagcgatat gttttgggga gatcggtatg
    12961 gccagctgga ggatcctttc ggacaccggt ggtcggtcgc gacccatgtt cgtgacgtcg
    13021 acccacaagc attgcaggag gcggcacgga aggcttgtga gtaggaatct tttacggcgt
    13081 gcaatacatc cacatggaga tctgacatgc gctacgtcga tggttttgtg ctggcggtcc
    13141 cgaagaagaa tgtgaatgcg tattttaaga tggccaagaa ggcctgcaaa gtctggacgg
    13201 agcatggtgc attggaatac cgcgaatgtg tgggcgacga tctcgcggtc aagatgggcc
    13261 tgccgtttcc gcgactggcc aaactcaagc aaggggaaac cgtggttttt tcatggatcg
    13321 tctacaagtc gcgaagtcat cgcgaccgtg tcaacgccaa ggtgatgaag gatccgcgga
    13381 ttaatgaaat gtgtgatgaa aaggccatgc ccttcgatgt caagcgcatg gcctatggcg
    13441 gattcaaggt gatggtggat ggaggaacac ggtcatgagg ccgttgcctc attgatcact
    13501 tgatcacatg ctcacggttt cttggctgca tggacaacga taaatgactt ggagatctgt
    13561 tgccggaaag ggtgagccgg cccatgtcga ttcaggcaag cgccgacgac tactttgtga
    13621 aggcggcaca catcgtcgat ctctgctcac actgacctga tggaggattc catgcgattt
    13681 atgatcatca tcaaggcgac caaagattcg gaagccggcg tcatgccgag ccagcaattg
    13741 ctgagcgaga tggggacgtt caacgaggaa ctcgtccaag ccggcgtgat gctggcaggg
    13801 gacggactcc atccaagtac caaaggggcg cgcgtccgat tctcaggcac ccgacgcacc
    13861 gtcatcgacg gcccctttgc agagacgaag gaactgatcg cgggctattg gttatggcaa
    13921 tgtggctcca aagaggaggc gatcgagtgg gtcaaacgtt gtcccaatcc gatgcccggc
    13981 gaatccgaga ttgaaattcg ccaggttttc gaggcggagg acttcggtgc cgagttcacc
    14041 ccggaactgc gagagcaaga agatcgcctg cgtgcgcaga tggcacagaa ccactaggca
    14101 aggtctgatt tcatctgttg tgcattgacc agcccattgc cacgtgattg actagagtca
    14161 gagacgctgt gccatccaga gatgttgcac agtaccgtgt agaaacctga cgaatcggat
    14221 ggcgccacgg gatcgacggc gccatcttac ctataaggag gccatcatgc gcgtgacatc
    14281 cattctgctt gcctgtctct gcacgtccct catgaccatg cccgtctctg ctaaggagaa
    14341 aaagtcggag aaagccatgg atcctcaagc catgatggaa gtgtggaaga aggcggccat
    14401 gccgggagag cctcataagc tgtttgccag tctcgccggg agttggacga ctcagaccaa
    14461 agaatggatg gagccgggga agccgccgtc cgaaaccacc ggcaccgcgg aaatgaagat
    14521 gttgttggac ggacgatttc tctaccagga attcaacggc actatgatgg ggcaaccctt
    14581 cgccgggatc ggcatcgacg cgtatgacaa tatgaccaag aaatacgtca cggcctggat
    14641 ggatagcatg ggaaccggca tttttaccat gcaaggcacg tccagtccgg acgggaaaac
    14701 gatcacgctg aagggctcgc atccggaacc gggcggcggg cagatgcatc accgtgcggt
    14761 gtggaaaatc attgatcaga attcacagac gtttgaaatg tacggcactc atgggcatgg
    14821 gaaggaaatg aagttcatgg agatcaccta tacgagaaag tcatagcact cgcgcctctc
    14881 gcagcggacg cggatcaccg catcggccca tcatcaaatg actgccgggg atgctcattc
    14941 ctagtgtctt gggatgggga tccccgcaac cgatgggatg gtagaagttc tgttccggct
    15001 gtcgctgcca tctctttgtg ttgtcaccgc aacatgtggc gctgtgggct gattatgcgg
    15061 cgatcgtatt tctcatagtc cccccatcgt caccatcccc ctgccgaaat gttttattga
    15121 tgtagcgccg agccgaccgt gatccgtcgt gtgtgcagga acaggctcgt tgcttacatg
    15181 ccagccaacc agtaagatgg gtacgcaatc atctccgccc gcgcggcaat tcagattgta
    15241 gtcaggagta cgcatgatca ggcgatggcg atggtccact atagccccga tggtgtgcgg
    15301 gtggctcgcg ttggcgcttg tgccgggttg caagcaggaa gccggctcgg cgccggcacc
    15361 gaccgttcct gaggtgggaa ttgtggtggc gaccgcccag gatgtgcctg acgaaccgga
    15421 gttcatcgga caggcggaat cgtcacgccc cgtggagatc cggtcgcagg tcaccggcat
    15481 cttgaaacag tggtacttca aggaaggccg cgacgtcaaa aaaggggatc gtctgtatca
    15541 aatcgatccc gtgcccttcc atgcggcgat gctgagtgcc aaggctaaag tcgcccaatc
    15601 ggaagcccgg ttggtccagg cgaatcagaa tctgacacgc gtgaagccgc tcctggcaga
    15661 acaggcggtg agcacgaaag atgtggacga tgccgtcgcc gaacagttgg ccgcgaaagc
    15721 cgccctcgaa ggcgcgaagg ccgaactcgt caaagcaaaa ttcgatctcg ataacacgct
    15781 gatcatcgcg cccatcgacg gaatgatcga acgaacgcgt gtgtatgaag gccgactagt
    15841 gtcggcacag acagacttgc tgaccatgat tcatcaagtc gatcccatgt acgtgatcgt
    15901 cagtgcgccg gagagttttc tgttgaagcg ccggcgggac acggatgcca aacgtattca
    15961 gcatccgggg gtgtaccaac tgcgtggcgt gctcaccttt gtcgatggga cgacgtacgg
    16021 gcacgaaggc gtactggatt tgctggatgt gggattaaaa actgatacgg gatcgagaca
    16081 ggcgcgcgtc gtatttccca acccggaccg ggtgttgttg ccaggacagt tcgtgcgagt
    16141 gcggttcaag ggcacgctca agaccggcgc catcctggtg ccgcaacgcg ccgttcagca
    16201 gggcgccaag ggctccatcg tatttgtggt gggcaaggac gataaggtgg agatgcgtga
    16261 aatcctggcg agcagctggc aaggcagtca gtggatcgtc gaagaaggct tgcacgtcgg
    16321 cgatcgcatc atcgtggagg gcctgcataa aatcgctccc ggtgcggtgg tgaaaccggt
    16381 gcccgttccc gctgccggcg cggcaaccgc tccgagtgcc gcccccgccc cgccggagcc
    16441 cgcctcgtga tctcacattt ctttatcgac cgcccgatct ttgcgtcggt gttgtcgatc
    16501 gttatcatgg tgctgggctt ggtggcactg caggccctcc ccattgccca attccccgaa
    16561 atcacccctc ctgtcgtgca gatcgaagcc gactatccgg gcgccaatgc cgaaatcctg
    16621 gccgactcgg tggcgcggcc cattgaggtg caacttcccg gcatcgacaa tctgctgtac
    16681 tacgattcca ccagtacgaa cgacggccac atgtcgatca agttgacctt cgagatcggc
    16741 acggatgtcg atatcgcgca ggt
//
LOCUS       DAOQEW010000075        16661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_920_length_16661_cov_8.614597, whole genome shotgun sequence.
ACCESSION   DAOQEW010000075 DAOQEW010000000
VERSION     DAOQEW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_16661_cov_8.614597"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLS35_09785"
     CDS             <1..897
                     /locus_tag="PLS35_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI18997.1"
                     /translation="RWGMELGVQGGYDSERFAFLQGEREVSGADTLRHIHRANVSYLA
                     PIGKGLTITAGLFNSLIGYESLYAKDNANYTRSWIADNTPYMMFGVNAKYPVSDELTI
                     TAFVINGYYHLSHPNDQPSYGGQWAYKATPRLTLTQTLYGGPDQSNTALQFWRFYANH
                     IVEWKGDDLTLALSYDIGTENIADRPGHPRAFVTGGNMVARWHVAGPWTLVARPEFYW
                     DRNGRWTGSEQFVKAVTSTVEYKIPYKWTNTILRLEHRWDESTGAGGGFFRRGEVQPG
                     VLSLTPNQHLVLLGILWTFDSP"
     gene            complement(1151..1870)
                     /locus_tag="PLS35_09790"
     CDS             complement(1151..1870)
                     /locus_tag="PLS35_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI18998.1"
                     /translation="MHPLVLALILTITCTACTEARGLNRHILQESFHDHPDVTTDRDI
                     AATMAIQATLPTPYRLAIYFKHEDFPSRPALQRVDWVSSDGGRVQRSLEPVQEEGILQ
                     RTFLLANSTVQGNTLRDIRLAAARYNADAILIIDGASAFERYNNGHAWWYATGIGAYF
                     AHGTECHALFMMEGTLWDVRTGYLYGTQTAEGETTLIGPAPSLDDKAAVAEAKDVAMA
                     RFGRELADTLRLLKEKQKAIP"
     gene            complement(1896..2846)
                     /locus_tag="PLS35_09795"
     CDS             complement(1896..2846)
                     /locus_tag="PLS35_09795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI18999.1"
                     /translation="MTTRPLEQTARVAAPAHTRVGWIGTGVMGASMCRHLQAAHYRLT
                     LFTRNRGKASAILAKGATWADSPRAVAEQTDILITMVGFPRDVRQVYFGDHGVLAGAG
                     AGMVLVDMTTTEPSLAQEIAQAAQSRGVFAVDAPVSGGDIGARDATLSIMVGGDASAV
                     QTIMPLLECLGKKIVHQGHSGAGQHTKLCNQIVIAGTMIGVCESLLYGYKAGLELPRM
                     LESIRGGAAACWTLDNLTPRILARNFDPGFFVEHFIKDMGIALEEARRRQLTLPGLTL
                     AHQLYEKVEALGHGRSGTHALMLALEALSNIDPTRPSTSL"
     gene            complement(2850..3293)
                     /locus_tag="PLS35_09800"
     CDS             complement(2850..3293)
                     /locus_tag="PLS35_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19000.1"
                     /translation="MGTALLACLALCLSGGNRVFAQEAVTVQEVLADPALFHLRQITI
                     KGTVRQVQPLDPYEIPAGSTCYGGYLFSLEDDTATLSIAVPGLCGVPMVKDPDVEDGA
                     RVVVEATVQAPSHGGYALSFKGAKIAMDQEGVVQAIANRITPLVE"
     gene            3493..4857
                     /locus_tag="PLS35_09805"
     CDS             3493..4857
                     /locus_tag="PLS35_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNI19001.1"
                     /translation="MSDVQRQIETYIKDIRPQYEDMLGQAVEIPSISMDPRHAPDVRR
                     MAELATQYLRAAGAEAQIVETPGYPVVSGGWTVDPHYPTVTVYNHMDVQPAQEPEWKQ
                     APFAFQNDHGIYRGRGATDDKGPALAALFGARYAIDQGVPINVRFLWELEEENGSPSF
                     ASAIKNRAAIPAPDSVVISDTIWLSKNQPAMPYGLRGLLGARLTLRTGTKDAHSGVTG
                     GAARNPLVELMEIAHACVDAKTGKVKIPGFYDEVVPPTKAEIASFLKSGFQVSKFKQA
                     YGFKTLRTHDPAEVMRRIWAAPTFEVHGLTGGYHGPGVKTIVPGHGELKISMRLVPGQ
                     TPEKIFALLKHYVAKLNPAVKVEREGMLHPFRGVFDGPYVEAVKRAVLAGFGKEPAFI
                     REGGSIGAVVTMQKAWKVPILFMGLSLPEHGYHAPNEYFDWGQASGGIKAFAHYFSDL
                     AAKR"
     gene            5017..5982
                     /locus_tag="PLS35_09810"
     CDS             5017..5982
                     /locus_tag="PLS35_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNI19002.1"
                     /translation="MTVRNIRFRQSVIRTVLGLAAMSGLAMQPVESAFAKVVEVSMTA
                     VETKLVIDGEGHTYDAWTFNGQFPGPVIRVQEGDEVVFSLTNPETNGRPHSMDFHAAE
                     TNFLEHYKEVRPGESLQYRFKAKWPGVFAYHCGASPMIQHIARGMMGAIIVDPKDPKA
                     MPKADREYVLVQSELFKDPDDVDGMFDRKYQHVVFNGAVFRYDPVHSKAGGAFLEAKP
                     GERVRFYFVNAGPNNVSAVHPIAEIWDDVWESGNPANHTRGVQTQLIPPAGAAILDMV
                     LDGNDGVYPIVTHSLTDALRGAIALLKVDKDAKQLPLMPLVEPAK"
     gene            complement(6166..7212)
                     /locus_tag="PLS35_09815"
     CDS             complement(6166..7212)
                     /locus_tag="PLS35_09815"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNI19003.1"
                     /translation="MIDVKGYAARTAKSRLAPFTFSRRNVGRQDVLIGIRYCGICHSD
                     VHQARDEWGGSIFPMVPGHEIVGVVEQVGASVKQFKVGQMVGVGCFVDSCRTCPQCKK
                     GEEQYCEGHISFTYNGKERDGVTPTYGGYSTKVVVDQRYVLRIPKQLRPEEAAPLLCA
                     GITTYSPLHHWGVGKGHRLAIVGLGGLGHMAVKIGKALGAHVTVLSHSDKKRRDAKRL
                     GAKDFYKTSDPKTFTTLAKQFDFILDTVSAPHDLDAQLELLKTDGTMILVGVPDKPAQ
                     LGAFPLILKRRRLVGSLIGGIQETQEMLDFCAKHKCGADVEVIPIQKVNEAYDRLVRG
                     DVRYRFVIDMGSLT"
     gene            complement(7262..7687)
                     /locus_tag="PLS35_09820"
     CDS             complement(7262..7687)
                     /locus_tag="PLS35_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNI19004.1"
                     /translation="MAETSHPGSVQRTVGIACLTVCYLNLTACSLFGGTSQSLTVNSD
                     PPGANVLINGTSAGTTPLQYQVPRRGDLTVEVQKPGYTPQTRVTGRKLSSVGIVDVIG
                     GALFLLPLLGLIAPGAWEQDPGIIGITLEPDHPQPAQPQ"
     gene            complement(7838..9556)
                     /locus_tag="PLS35_09825"
     CDS             complement(7838..9556)
                     /locus_tag="PLS35_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19005.1"
                     /translation="MIRGNLCHHRQTIAILALALAFGSWAGIAESAPAVGGKGHAKTG
                     TPKTTPAIQTAIRYAEALSQGDRVTAGQLDFACQYRHLTASPTKVKQFAPKDDPSYDA
                     CWQNLTDAYAPMLTRSDIAMDIIWPSTGPLLFFGDDLPRAPASAFVADAVGVAPPGSG
                     LHVTALNSRTIPSGSFKLARTGKVLAAPTTLVQLSVQYQDPLTSPVTYAAGNVKWTNT
                     IKRPRRALKSVTLQWVVFSGLKQHGFPGDTAVFNLPVPSQPEAPGMMADKIPFTTETS
                     RALPESMAWWGPDDQPGVLTAAAARAAIFPDLRDRVALLNRVLIIDPKQPDALTVLTR
                     HLYSALLREARSSHQVPVKDPALGIVLDEFYWNIYAQGMRLDLSNGMEMGGLSQPTPA
                     DFLYRLIPALQTLAEIRPEQLDNRFRLGVAYRWNNDQIPMVETFEALVKDIPEHRRTP
                     KAEALLQLAWSRINKVSWNRILHDTETPRAYANAEASLAFAELPLDKFLAEYAMAYTM
                     IFLPNYGDKEKMLHHLTEAKRWFDEVPGKTNEAWRYFLHTGLLKAVLDADPIFAPILA
                     TVPSQP"
     gene            complement(9644..9994)
                     /locus_tag="PLS35_09830"
     CDS             complement(9644..9994)
                     /locus_tag="PLS35_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI19006.1"
                     /translation="MVLLIGTGILLAQPSDGLQRGQSLYREHCMDCHGTAGKGDGPKA
                     PFLSPRPGNLVSAATSAKTDKELLRTIAQGKPRTAMPGWKEALPEEDQQAVLQYIRSL
                     IQFSRSPTATPPTP"
     gene            10193..10318
                     /locus_tag="PLS35_09835"
     CDS             10193..10318
                     /locus_tag="PLS35_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19007.1"
                     /translation="MLAQPDRLAKGIEEYFAIRAVAEVRADFLADLAGQLVVQIG"
     gene            complement(10444..11076)
                     /locus_tag="PLS35_09840"
     CDS             complement(10444..11076)
                     /locus_tag="PLS35_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNI19008.1"
                     /translation="MASRKKSPPASSDGHGLPHSASHPFDQLYRDHVDVMYRFAYRLC
                     GEAEAAKDLVQETFLNAYRAYDRFRGDARVSTWLYAIASHACQRMRRKRKGEPERELS
                     LTEFVPTSEGEFALQIPMEGLSPQEALENKELREILDRAIAKLPRKYRMVLVLRDMEG
                     LSAKEVGDILGLSERAIKSRLHRARLFVRKELSGKGLAEEFHHAVTTVDR"
     gene            complement(11156..12202)
                     /locus_tag="PLS35_09845"
     CDS             complement(11156..12202)
                     /locus_tag="PLS35_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19009.1"
                     /translation="MVTLVNSDQRFRCISLSLSALLSLSCLPAAGWAEPSAPLIEPPP
                     LEEPAASVEPVAPEALPVEPEPTQTVATAPVSPSVQVSGFIWRSKPGIVFLQTPIGPL
                     SLSSKTTLRDVKNSQKITLWVHGTTTVIDIRERGADKLVHRYVTAMPNFTSPEKQTVA
                     LWTPDGEQSFALGGLAAKIASRSDEQPFTIEVDGSGTVRGLHDVQYDLQVNQVPRTPS
                     SVHLLLNGTVSKLKSNYVFLKTPLGIVTVSGKTGVRNAKVGQEMSVWVQDRHVAIDLY
                     QDGVSTPSRRFLSGPLTYAPEGQSALIMQGPEGAQSIPLAQRPAALSAVKEGLPITVE
                     LGRQGELLDVRRVN"
     gene            complement(12454..13848)
                     /gene="der"
                     /locus_tag="PLS35_09850"
     CDS             complement(12454..13848)
                     /gene="der"
                     /locus_tag="PLS35_09850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNI19010.1"
                     /translation="MLPSQAMSRTKPARGPVTPLFTLEGGRLPIIALVGRPNVGKSTL
                     FNRILGTRAAIVDDVPGVTRDRNYADATYRNRHFRLVDTGGLDPTASEGMLSLIRQQS
                     QLAIAEADILVLVLDARAGLTPPDEEVVATLRGSDKPVFYVINKVDTPKADPLVADFY
                     RLGQEHLYPLSAEHGIGVAELLDDLFPHMVDPPGEETPTEIPRIAIVGRPNVGKSTLV
                     NSVLGEARVVVSNVPGTTRDPVDSIATFKDRQYVLTDTAGIRRRGRVERGIEGYSVAR
                     SLRAIGRSDVAVLLLDAEEGVTEQDTKIAGLVLKQGRACILVVNKWDLKADDVDAREA
                     YKQDLERRFPFLAWAPVLFISALEQDFLRGLFALIDQVYFSFCKRVPTGALNQFFQGL
                     LEEHPLPVRKGKPAKASKSAFVTQVATRPPAFALFVGHPDNMTPAYLRFLENQIRKEY
                     HFSGTPIRLMVRKK"
     gene            13909..14595
                     /locus_tag="PLS35_09855"
     CDS             13909..14595
                     /locus_tag="PLS35_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNI19011.1"
                     /translation="MLQHRTDPTPHSVDWSHIDDVLLDMDGTLLDRHFDNFFFEEELP
                     RRYAAKHALAFAEARDRLMSMYRSVEGELAWTDLHYWTEQVQIDVVAMHRELDHMIGF
                     LPDAEAFLLSLRRLEKRVTILTNAHRAGVEVKTAKTGLHRQVDRIVDAFEVGWLKMRS
                     EYWPTCQELVGFDPSRALYIDDDEQCLAAAEQFGIRRIFHRSKSSSQASPVPSARFHS
                     IENFQSILPG"
     gene            complement(14640..>16661)
                     /locus_tag="PLS35_09860"
     CDS             complement(14640..>16661)
                     /locus_tag="PLS35_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI19012.1"
                     /translation="HSGHPQQAFPVMTEHAAGTFAVERASIWLFDGTQSTLVLQDLFE
                     ATSNRHSQGTMLSTKQYPAYLHALETEPYSLAAHAAQSDPRTSELTPSYLIPLGITSM
                     LDAPIRRHGRVVGVLCLEHTGTPKTWTSEEEAFAASLAAMATLTLEAADRRQAQEALE
                     QHVRERTGELRRMTAHLQTIIEESPLAMIELDHTGHVTTWNAAATALFGWTREEVLGK
                     ELPYVPAGQEQASNELWTSVMNGGAPRNLELCRQRKDGTLIDVNMWGTLLQGPGGQTT
                     GSIGFFIDVTEHKQLEEQLRQAHKMEGIGRLAGGVAHDFNNLLTVINGCAMLLLEQVR
                     AEDPLHRSLTEILTAGQRAAALTKQLLAFSRRQVLTFQVFDLNEALSSISSMIERLIG
                     EDIALVRDLTSQPCIIRADRGQIDQVVLNLAVNAKDAMPHGGTLTMATRTVTLTADTP
                     VPHPAMLPGRYVHLSIRDSGEGMDRTTLSHIFEPFFTTKEEGKGTGLGLATVYGIVKQ
                     SQGFIFADSNPGDGATFDMYFPLAQAPSPAAAPATPSRPHCGHETILLVEDQTAVRML
                     LTQALSDYGYTLIEASSGQEALRLVAAAKTPIHILLTDVVMPQMTGPALAERLRQQWP
                     SLRVLFMSGYAEGSVLPTFLAEPGTGFIQKPFLPTELAQKLRELLDSAK"
BASE COUNT         3337 a         4603 c         5232 g         3489 t
ORIGIN      
        1 cggtggggca tggaactcgg cgtgcaagga gggtacgact ctgagcgctt tgcatttttg
       61 cagggggaac gggaagtcag cggggccgat accttgcggc acatccatcg cgcgaatgtg
      121 tcctatctgg caccgatcgg caaggggctt accatcaccg ccgggttatt caacagtctt
      181 atcggctatg agtcgttgta tgcgaaagac aatgcgaact acacgcgttc ctggattgcg
      241 gacaatacgc cctacatgat gttcggcgtc aatgccaaat atcccgtcag cgatgagttg
      301 accatcaccg cgtttgtgat caacggctat taccatctct cgcatcccaa cgatcagccc
      361 agttatggcg gtcaatgggc ctacaaggcc acacctcggc tgacgctcac gcaaactctc
      421 tatggtgggc cggatcaaag caataccgcg ctgcagttct ggcggtttta cgcgaaccat
      481 atcgtggaat ggaaaggcga cgatctcacg ctggcactgt cctacgacat cgggaccgag
      541 aacatcgccg atcgtcccgg ccatccgcgg gcctttgtga ccggcggaaa tatggtagcc
      601 cggtggcatg tcgccggccc ctggactctg gtggcgaggc cggagttcta ctgggaccga
      661 aacgggcggt ggacgggatc ggagcaattc gtgaaagcgg tgacgtcgac cgtggaatac
      721 aagattccct acaaatggac gaataccatc ctgcgcctcg aacatcgatg ggacgaatcg
      781 accggagcag gaggcggatt ctttcggcgt ggagaggtgc aacccggcgt gctgagtctc
      841 acgccgaatc agcacctggt gttgctcgga attctatgga cgttcgatag tccgtgagcg
      901 ggcgctgaaa atggcttcgg gcttcgttct cggcgcactc gtgacctcga cgtactgcag
      961 agagtacgcc tcagtccctc gcgtcctgcg gcctcgcacg aaagccattt tgagcgccca
     1021 ggagaccgag gtgtagttgc gagctgttgc tggttcggat caaccgcggc ggatttcggt
     1081 ctcaactgct tcgcgaccat acgcaggcag catctcagtc acaactggaa gcagaaactg
     1141 aggagggcgt tcacggtatt gccttctgct tttccttcag cagccgcaat gtatcggcga
     1201 gttctctgcc gaagcgggcc atcgccacat ccttcgcttc ggccaccgcg gctttgtcgt
     1261 cgagtgaggg ggcgggaccg atgagcgttg tttctccttc cgccgtttgc gtgccgtaga
     1321 gatagcctgt ccggacatcc cacaacgtgc cttccatcat gaacagggca tggcattcag
     1381 tgccgtgggc aaagtaggcg ccgatgccgg tggcatacca ccaggcgtgg ccgttgttgt
     1441 accgttcaaa agccgaggcc ccgtcgatga tgagaatcgc atcggcgttg tagcgggccg
     1501 cggcgagccg tatgtcacga agggtattgc cttgcacggt tgaattggcg agcaagaagg
     1561 ttcgttgaag aatgccttcc tcctgcaccg gttccagtga ccgttgcacg cggccgccat
     1621 cggaactgac ccagtccact cgttgcaagg cgggacggct ggggaaatcc tcatgtttga
     1681 aataaatggc gaggcgatac ggcgtcggca gcgtcgcctg gattgccatg gtggcggcga
     1741 tgtcgcgatc agtcgtcacg tccggatgat catgaaagga ttcctgcaag atgtgccggt
     1801 tcaggccacg ggcttcggtg caggcggtgc aggtgatggt gaggataagt gccagaacaa
     1861 gaggatgcat acccgttctc atgcggctcc tgtgattaga gtgaggtcga gggtctggtt
     1921 ggatcgatat tagaaagggc ttccagcgcc agcatcaacg cgtgcgttcc ggagcggcca
     1981 tggcccagcg cctccacctt ctcgtacagt tgatgtgcga gcgtgagtcc tggaagggtg
     2041 agctgccggc gccgtgcttc ctcgagagcg atgcccatgt ctttgatgaa atgttcgacg
     2101 aagaagccgg ggtcgaagtt gcgagccaga atgcggggcg tcaaattgtc cagcgtccag
     2161 caggcagcgg cgccgccgcg gatcgattcc agcatgcgcg gcaattcgag tccggcctta
     2221 tagccgtaca gcaggctttc acagacgccg atcatcgtgc cggcgatcac aatctgattg
     2281 cagagcttcg tgtgttgacc ggctcccgaa tggccttggt gaacgatctt tttgcccaga
     2341 cattcgagca gcggcatgat cgtttgaacg gcgctggcat caccaccgac cataatcgat
     2401 aacgttgcgt cgcgcgcccc aatatcccct ccggagaccg gcgcatcgac ggcaaacact
     2461 ccgcgcgatt gggccgcctg tgcaatctcc tgtgcgagtg agggttcggt ggtggtcata
     2521 tcgaccagca ccatgcccgc gccggcgccg gccagaacac catggtcccc gaagtacacc
     2581 tggcgcacat cccgaggaaa tcccaccatg gtgatgagga tatcggtctg ctcggccacc
     2641 gcgcgaggag aatcggccca ggttgctcct ttggcgagaa tcgctgaggc tttgccgcgg
     2701 ttgcgtgtaa acagcgtaag gcgatagtgg gcagcctgga ggtgacggca catggaggcg
     2761 cccatcacgc cggtgccaat ccagccgacg cgcgtatggg cgggggccgc gacgcgggca
     2821 gtctgttcaa gtggacgagt cgtcatcgtt tattccacca atggcgtgat ccggtttgca
     2881 atggcttgta caacgccttc ttgatccatg gcgattttgg cgcctttgaa gctcaaggca
     2941 taccctccat ggctgggagc ttggaccgtg gcctcgacca cgacgcgggc accgtcttcc
     3001 acgtccggat ctttcaccat cggcacgccg catagtccgg gtacggcgat ggacagggtg
     3061 gccgtgtcgt cttccaggct gaacagatag ccgccgtaac aggttgagcc ggccgggatc
     3121 tcgtagggat cgagcggttg gacctgacgc acggtgccct tgatggtgat ttgtcgcaaa
     3181 tgaaatagcg cgggatcagc gagcacttcc tgcacggtca cggcttcttg cgcgaatact
     3241 ctgttgccgc ctgaaagaca caatgccagg caagccaaca gtgctgtgcc catacgaagt
     3301 gcttggtgag aagtgttatc gtgcatcggt ttcattctac atgctttctc gccatcgtcg
     3361 ccaacatagg ctttcctgtt ccgactggat ataatcgccg tgactgggat tgggtcctac
     3421 gtagtgagtg tgatgcagag aaatgatgtg agggaattcg ggcactgcca ctgagatcgg
     3481 ggaggagcat caatgagcga cgtgcaacgg cagatagaga catacataaa ggatatccgt
     3541 ccgcagtatg aagacatgct cgggcaggcg gtcgagattc cgtccatcag catggacccc
     3601 cgccatgcgc cggatgtgcg gcgcatggcc gaactggcga cacagtatct gcgggcggct
     3661 ggtgcggaag cgcagatcgt ggaaacgccc gggtacccgg tcgtctcagg cggctggacg
     3721 gtcgatcctc actatcctac cgtgacggtc tacaatcata tggacgtgca gccggcgcag
     3781 gaaccggaat ggaagcaggc tcccttcgcc ttccagaatg atcacggcat ctaccgtggg
     3841 cgaggggcga cagatgacaa ggggccggcc ctcgcggccc tgttcggcgc gcgatatgcg
     3901 attgatcaag gggtcccgat caatgtgcgg tttctctggg aactggaaga agaaaacggc
     3961 agtcccagtt ttgcctccgc gatcaagaac cgggcggcga ttccggctcc ggattcggtg
     4021 gtcatttcgg acaccatctg gctctccaag aatcaaccgg cgatgccgta tggattgcga
     4081 ggattactcg gcgcacggct gacccttcga accggaacca aggatgcgca ctccggcgtg
     4141 accggcgggg cggcccggaa tccgctcgtc gagttgatgg agattgccca tgcctgtgtc
     4201 gatgccaaga ccggtaaggt gaaaatcccc ggattctacg acgaggtcgt gccgccaacg
     4261 aaggcggaga tcgcgagttt cttgaaatcg ggtttccagg tcagcaagtt caagcaagcg
     4321 tacgggttca agaccttgcg cacgcacgac ccggcggagg tcatgcggag aatttgggcc
     4381 gccccgacat tcgaagtgca tggccttacg ggtggttatc atggccccgg cgtgaagact
     4441 atcgtgcccg gccatggcga actgaaaatc agcatgcgac tggtccccgg tcaaacgcct
     4501 gagaagatct ttgccctgtt gaagcactac gtggcgaaac tgaatcccgc ggtgaaggtg
     4561 gagcgagagg gtatgttgca tccgttccgt ggggtgttcg acggtcccta tgtcgaagcg
     4621 gttaagcgtg ccgtcctggc cggattcggc aaggagccgg ccttcatccg tgaaggcggt
     4681 tcgatcggtg cggtggtgac gatgcaaaag gcgtggaaag tgccgatcct gtttatgggt
     4741 ctcagcctcc ccgagcatgg atatcatgcg ccgaatgagt acttcgattg ggggcaggcc
     4801 tccggcggca tcaaggcctt cgcccattat ttttcggatt tagctgccaa gcggtaatgc
     4861 cctggccgaa gatcccgata ggcgatcggc gccagccggc gcgacatttt taaaacatga
     4921 caaatgtcat acttgatttg tcgcgttgcg cggtatcctc accacggttc tgggtgaacc
     4981 gagaccaatc actttcacag gaggagccgc agcattatga cagtacgcaa tatccgcttt
     5041 cgtcaatctg tgatccgcac ggttttaggg ttggcggcaa tgtcaggcct cgccatgcaa
     5101 ccggtcgaat ccgcgttcgc gaaagtcgtc gaagtgtcca tgacggccgt ggaaaccaag
     5161 ctcgttatcg acggcgaggg acatacatat gatgcctgga cattcaacgg gcagttcccg
     5221 ggcccggtga ttcgggtgca ggaaggcgat gaagttgttt tctcgctcac gaatccggag
     5281 accaacggcc ggcctcactc catggatttt cacgccgccg aaaccaattt cctggagcac
     5341 tacaaggaag tgcggccggg tgaatctttg caatatcggt tcaaggccaa atggccgggc
     5401 gtgtttgcct atcactgtgg cgcgtctccc atgatccagc acatcgcgcg cgggatgatg
     5461 ggcgccatca tcgtcgatcc caaggatccc aaggcgatgc cgaaggcgga tcgagaatat
     5521 gtgttggtgc agagcgagct gttcaaagat cctgacgatg tcgacggcat gttcgaccgg
     5581 aaatatcagc acgtcgtttt taacggggcg gtgttccggt atgaccccgt gcatagcaag
     5641 gcgggaggcg cctttttaga agccaagccg ggtgagcgcg tccggttcta tttcgtcaat
     5701 gccggaccga acaatgtgtc cgcggtccat ccgattgccg aaatctggga tgatgtctgg
     5761 gaaagcggca accccgccaa ccatacgcgc ggagtacaaa cgcaactcat cccgccggcg
     5821 ggagcggcca ttctcgatat ggtgttggac ggcaatgacg gtgtgtatcc catcgtcacg
     5881 cactctttga cggatgccct tcggggcgcg atcgcactgc tgaaggtgga caaggacgcg
     5941 aaacagcttc ctttgatgcc gttggttgag cctgccaagt aagttcctgt gtctttgagg
     6001 aaaggccggg cggctacagc cgcccggcct gcctctctcc tcggtagact cacattcaac
     6061 acattgagac tgttgacgtg taccggctcc gtgctgctga tagctgggca tgccgaggcg
     6121 acatcggacg atggatgtcg cgtgcaacga agaagacgtg gtgcgttagg tgagggaacc
     6181 catgtcgatg acgaagcggt accgcacgtc cccgcgaacg aggcgatcgt aggcttcatt
     6241 caccttctga atcgggatga cttccacgtc ggcgccgcat ttgtgcttcg cgcagaaatc
     6301 cagcatctcc tgtgtttcct gaatgccgcc gatcaatgag cccaccagcc tccgccgttt
     6361 gaggatcaac ggaaaagccc ccagttgcgc cggtttgtcc ggcaccccga cgagaatcat
     6421 ggtgccgtcg gttttgagca attccagctg tgcatccagg tcatggggcg ccgatacggt
     6481 gtccagaatg aagtcgaatt gtttggcgag ggtggtgaag gttttggggt cagaggtctt
     6541 gtaaaaatct ttggcgccca atcgtttggc atcgcgccgc tttttgtcgg aatgactcag
     6601 cacggtcacg tgagcgccca aggcttttcc gattttgacc gccatgtgcc ccagccctcc
     6661 cagcccgaca atcgccaggc ggtgcccctt tcccactccc caatggtgta acggcgagta
     6721 ggtcgtgatg ccggcgcaga gaagcggggc ggcttcctcg ggacgcaatt gcttagggat
     6781 gcgaagcacg tagcgctgat cgaccaccac tttggtggag tagccgccat aggtcggcgt
     6841 gacgccgtcc cgctccttcc cattgtaggt aaagctgatg tggccttcgc agtactgttc
     6901 ttcgcctttt ttgcattgcg ggcaggttcg gcaggaatcg acgaagcatc ccacgccaac
     6961 catttgaccc accttgaatt gtttcacgga ggcgccgacc tgttcgacaa cgcccacgat
     7021 ttcatgtcca ggtaccatgg ggaagatcga gccgccccat tcatcccgtg cctgatgcac
     7081 gtccgaatga caaatgccgc agtagcggat gccgataagc acgtcctgcc ttcccacgtt
     7141 tcttcgtgag aacgtaaacg gagccagtcg ggattttgcg gtgcgggcgg cataaccttt
     7201 gacgtcaatc atagtcctgt tccttacggt ctgatcgtac agcgtaatcg tgggtccgaa
     7261 gttattgagg ctgcgcgggt tgagggtgat ccggttcgag tgtgatgccg atgatgcccg
     7321 ggtcttgttc ccaggcgccc ggcgcaatga ggcccagcag cggcagcaag aacaaggcac
     7381 caccgatgac atcgacgatg ccgacactac tgagtttccg gccggttact cgggtctgcg
     7441 gtgtgtaccc gggcttttgt acttcgactg tcaggtctcc gcggcgtgga acctggtact
     7501 gcagcggggt tgtgccggcc gaggtgccgt tgatcaagac attggcgccc gggggatctg
     7561 aattcaccgt cagtgattga gaagttccgc caaagagaga gcaggccgtc agattcagat
     7621 agcaaaccgt gaggcaggcg ataccaacag ttctttgcac gcttccagga tgtgatgttt
     7681 ccgccatctg cgactcccct tcaatgctgt cgggtcatcg ttaccacagc ttgtccagct
     7741 tgacaaggta cggaggggcg gatgcgacga gagagtgatg gcgcggccaa tcggatggcg
     7801 aaggctttgg ccttcgccat ccgatcagtg accatgatta cggctggcta gggactgttg
     7861 cgaggatagg tgcaaaaatg gggtcagcat ccagcaccgc tttcagtagg cctgtatgga
     7921 ggaaatagcg ccaggcctcg ttcgttttcc ccggcacttc atcgaaccag cgtttggctt
     7981 cggtgaggtg atggagcatc ttctccttgt ccccatagtt gggcagaaag atcatggtgt
     8041 aggccatggc atattctgcg aggaacttat ccaagggaag ttcggcaaat gccagtgagg
     8101 cttcggcatt ggcataggct cgcggagttt ccgtgtcatg caaaatccgg ttccaggaca
     8161 ccttgttgat acgagaccag gccagttgca agagggcttc ggctttgggt gttctgcgat
     8221 gctccggaat atccttgacc aacgcttcga aggtttcgac catcggaatc tgatcgttgt
     8281 tccaccgata agcgactccc aagcgaaacc ggttgtcgag ttgttccggt ctgatctcgg
     8341 ccaaggtttg aagagccggt atgaggcgat aaagaaaatc tgccggcgtg ggttgcgaga
     8401 ggccgcccat ttccatgccg ttggagaggt ccaggcgcat gccttgtgcg taaatgttcc
     8461 aataaaactc atccagcacg atgccaagcg ccggatcctt gaccggcact tgatgactgc
     8521 ttctcgcctc ccgcagcagc gcgctgtaca gatggcgggt cagcaccgta agagcatcgg
     8581 gctgtttggg atcgatgatc aagacacggt tcagcaaggc gacccggtcg cgcaggtctg
     8641 ggaagatggc ggcccgtgcc gccgccgcgg tcaggacacc cggttggtcg tccggtcccc
     8701 accaggccat ggattcaggt aacgcgcggc tggtttctgt cgtgaaggga attttgtctg
     8761 ccatcatgcc gggtgcttcc ggctgtgaag gaaccggcag gttgaacacc gccgtatccc
     8821 caggaaaacc atgttgcttc agcccgctga agacgaccca ctgcagggtg acggatttca
     8881 atgctcgccg cggccgcttg atcgtattcg tccacttcac gttcccggca gcataggtga
     8941 cgggcgatgt gaggggatcc tgatactgca cggacagttg gaccagggtg gtcggagcgg
     9001 ccaggacttt tccggtgcgc gccagcttga aggacccgga ggggatcgtg cggctgttca
     9061 aggcggtcac atgcagtccg ctgcccggag gtgcaacacc cacggcatcc gccacaaacg
     9121 cggaggcagg cgctctgggt aggtcatcgc cgaagaacag cagcggaccc gtgctcggcc
     9181 agatgatatc catcgctatg tctgacctgg tgagcatggg ggcataggcg tcggtcaggt
     9241 tctgccagca cgcgtcgtaa gacggatcat ctttcggcgc gaattgtttc accttcgtcg
     9301 gcgaagcggt gaggtgccga tactggcagg caaagtccaa ttggccggct gtcacgcgat
     9361 cgccctgcga aagtgcttca gcataccgta tggctgtttg tatcgccggt gtggttttag
     9421 gggtgccggt cttggcatgt ccctttccgc ccacggccgg cgcggattca gcgattccag
     9481 cccaggagcc gaatgcaagc gccagggcaa ggatggcaat ggtctgtcgg tgatggcaca
     9541 ggttcccgcg gatcatacga aggacctccg atgaggataa aagaaggtca ctctatcacg
     9601 cggctctttt cagacgaaag aggcctggct taagaacacg tgttcaggga gtcgggggag
     9661 tagcggtggg agaacgggaa aattgaatca gggagcgaat gtattggagc acggcttgtt
     9721 gatcttcctc cgggagcgcc tccttccagc cgggcatggc tgtgcggggt ttgccttgag
     9781 caatcgtccg gaggagctcc ttgtctgtct ttgcagaggt ggctgcagag acgagattcc
     9841 ccgggcgcgg cgataaaaac ggagccttgg ggccatcgcc cttccccgcg gtgccgtggc
     9901 aatccataca atgttcacga tacagggatt ggccgcgttg caggccgtca gagggctggg
     9961 cgagcaggat gccggtgccg atgagcagga ccatgatcgg aaggccgctg gaacatgcgt
    10021 gaagaaaccg cgacgctgag cgagtctgtg agagggaatc agttgccgac ggctcaggca
    10081 cagccgtgat gggattcgga cgcgtcacga tggctcacac cgtcgcggcg gcctttaaga
    10141 tctggcgtcg aagtgcggct cgatcgtgct cagagaggga gggggagggc cgatgctggc
    10201 acagccggac cgtctggcga agggaatcga ggaatatttc gcaattcggg cagtcgccga
    10261 ggtgcgcgcg gatttcctgg cagatctcgc cggacagctc gtcgtccaga taggctgaaa
    10321 ggcgttccag aactttgacg caattccgtt tgcggtgacc ctgatgcggg gtgggcttgc
    10381 gagtgcctgg atatgtgcgt acgcggtctg ccatggtcat actgcttctt tgactatacc
    10441 aaactatcga tccacagtgg taaccgcgtg gtgaaattcc tccgcgagcc cttttccact
    10501 gagttctttt ctgacaaaca gacgggctcg atggagccgg gatttgatgg cgcgctcgct
    10561 caatccgagg atgtcgccca cttctttggc gctcaaccct tccatgtcgc gcagaacgag
    10621 gaccatacga tacttgcgcg gtaatttggc gatggcccgg tctaagatct cgcgcagttc
    10681 cttattctcg agcgcttcct gagggctcag tccctccatt ggtatctgta gggcaaactc
    10741 gccttccgac gtcggcacga attcggtcag cgacagctcg cgttccggtt cgcctttgcg
    10801 ttttcggcgc atgcgctgac aggcatggga ggcgatggca tagagccagg tcgacacgcg
    10861 cgcatccccg cgaaagcgat cataggcgcg ataggcgttg agaaatgttt cctggaccag
    10921 atctttggcc gcttcggctt cgccgcacag ccgataggca aagcggtaca tgacgtcgac
    10981 atggtctcga tacagttggt cgaatggatg ggatgcggag tggggcaggc cgtgcccatc
    11041 agatgacgcc ggcggacttt tcttccgtga tgccatacgc ggaagcgagt ctacgggtgg
    11101 tgttcagacc ctgtcaagcg aacggccggg tcgacaccca cgttgatggc gatgactaat
    11161 tgacgcgccg gacgtcgagg agttcgcctt gccggcccag ttcgaccgta atcggcaagc
    11221 cttccttcac ggccgagagg gccgccggcc gttgcgcgag cggaatggac tgtgcgccct
    11281 cgggtccctg catgatcagt gcgctctgcc cttccggcgc ataggtcaac gggccggaga
    11341 gaaagcggcg ggatggagtg gacacaccgt cttgatacag gtcgatggcc acgtgccgat
    11401 cctgcaccca caccgacatc tcttgcccga ctttggcgtt gcggacaccg gttttcccgc
    11461 tcacggtgac gattcccaga ggggttttga gaaacacata gttcgacttt agcttcgaaa
    11521 ccgtgccgtt caagagaaga tggacggagg atggtgtgcg cggaacctga ttcacttgaa
    11581 gatcgtattg cacgtcatgc aggccgcgaa ccgtgccgga tccatccacc tcgatggtaa
    11641 aaggctgttc gtctgaacgg ctggcaattt tcgctgcgag cccgccgagg gcgaacgatt
    11701 gctcgccgtc aggggtccag agcgcgaccg tctgtttttc gggggaggtg aaattgggca
    11761 tggccgtcac ataccgatgc accagcttgt cggcccccct ctcgcggata tcaatgactg
    11821 ttgtcgtgcc atgaacccag agtgtgatct tttgcgaatt tttgacgtcg cgcagcgtcg
    11881 tttttgaact cagagaaaga ggtccgatcg gtgtttggag aaagacgatt cccggcttgg
    11941 atctccaaat gaaaccggat acttgcacgg aaggggaaac gggcgcagtg gccacggtct
    12001 gggtcggttc cggttcaacc ggaagcgcct ccggtgcaac tggttcaact gaggcagccg
    12061 gctcttcgag tggaggtggt tcgatgagcg gggcggaggg ttcagcccag cctgctgcgg
    12121 gcaggcagct cagagaaagc agcgcactca aggagaggga aatgcatcgg aatcgttggt
    12181 cggaattcac gagagttacc ataatgtgta tcgaatgatg cggtgtggaa tggtttcacg
    12241 ccgattgagc gggggagaaa ccggccatta ctagtgaatg gccggttctg ccggatcgta
    12301 cgtggcgtac aacgtctcct gtgcgaataa catgctggtc ggcatgagtc aagttgaaag
    12361 gccttcggga cctctatgta ggtcattcag gaagtgggat atgcgacgga ctgtagcagg
    12421 agcgccacgg tctgcgatgc tgtctcccgc gcactatttt ttgcggacca tcaaacggat
    12481 gggggtgccg gaaaaatgat attccttccg gatttggttc tcaagaaaac ggagataggc
    12541 cggcgtcatg ttgtcgggat gtcccacgaa cagcgcaaag gcgggaggac gtgtggccac
    12601 ttgcgtcaca aacgccgatt tactcgcttt ggccggcttg cctttcctca ccggcagcgg
    12661 gtgttcttct aacagaccct gaaaaaactg gttcagcgcc cccgtgggaa cccgtttgca
    12721 gaaggagaaa tagacctgat cgattaatgc gaagaggccg cgcaagaaat cctgctccag
    12781 cgccgagata aagagcaccg gcgcccaggc gagaaacgga aagcggcgtt caaggtcctg
    12841 tttgtacgct tctcgagcat ccacgtcatc ggccttgaga tcccatttgt tcacgacgag
    12901 aatgcaggcg cgaccctgtt tgaggacgag gcccgcaatc ttggtatcct gttccgtcac
    12961 accttcttcc gcgtcgagca gaagcaccgc gacatctgac cggccgatgg cccgaaggga
    13021 gcgcgccaca ctgtagccct cgatcccgcg ttcgacacgg cctcgccggc ggataccggc
    13081 cgtgtcggtc aggacatatt gccgatcttt aaaggtggca atcgagtcta ccggatcgcg
    13141 ggtggttccg ggcacgttgc tgaccacgac ccgcgcctcg ccgagtactg aattgaccag
    13201 ggtggatttc ccgacgttcg gacgcccgac gatggcaatg cgggggattt ccgtcggggt
    13261 ttcttcaccc ggcgggtcga ccatatgggg aaacaaatcg tcgagcaatt cggccacgcc
    13321 gataccatgt tccgctgaga gcgggtacag gtgctcttgc cccaggcgat agaagtcggc
    13381 cacgagcgga tcggccttgg gcgtatcgac cttgttgatc acataaaaca ccggcttgtc
    13441 ggagccgcgc aacgtcgcga ccacctcttc atcgggcggc gtgagcccgg ctcgagcatc
    13501 gaggacgagg actagaatgt cggcctccgc gatggcgagt tgagattgtt gccggatgag
    13561 cgacaacatt ccttcagagg ccgtcggatc gagtccgccg gtatcgacta agcggaaatg
    13621 gcggttgcga taggtggcgt cagcatagtt gcgatctctg gtcacgccgg gcacatcgtc
    13681 cacaatggcg gctcgtgtgc cgagaatgcg attgaagagg gtcgatttac ccacattcgg
    13741 acgtcccacg agggcgataa tgggcaggcg accgccttcc agcgtgaaga gcggtgtgac
    13801 gggaccacga gcgggtttgg tgcgtgacat ggcctgcgac ggtaacatag aaatttcgtt
    13861 gaacacaatg actcgtcttg tcttaccggt ctccgttata ctgaactgat gctgcagcac
    13921 cgcacggacc cgacgcctca ttccgtcgat tggagtcaca tcgacgatgt cctcctcgac
    13981 atggacggca cgctcctgga ccgccatttc gacaactttt ttttcgaaga agaactgccc
    14041 agacggtacg cggcgaaaca tgcattggcc ttcgcggaag cccgggatcg actgatgagc
    14101 atgtatcgtt ctgtagaggg cgaactcgcc tggactgatc tacattattg gaccgagcag
    14161 gtacagatcg atgtggtggc gatgcatcga gaactcgacc acatgattgg attcctcccc
    14221 gatgccgagg cgtttctcct gtcgttgcgg cggctcgaaa agcgggtcac gattctcacg
    14281 aacgcgcacc gggcgggcgt tgaggtcaaa actgccaaaa ccggtctaca ccggcaggtc
    14341 gaccgcatcg tggatgcgtt cgaggtagga tggctgaaga tgcgctccga atactggccg
    14401 acctgtcagg agctggtcgg cttcgatccg tcacgggctt tgtatatcga tgatgacgag
    14461 caatgtctcg cggcggcaga acagttcggc atccgccgga tctttcatcg ctcgaagtcg
    14521 agctctcaag cctcgccggt tccttccgca cgattccact ccatcgaaaa tttccaatcg
    14581 atcctgcccg ggtgattcac gggcgtggcg ctcgcgcacc cgtgtcactc accgagtggc
    14641 tacttggcgg aatcgagtag ttcgcgaagt ttttgggcga gctccgtcgg taaaaacggt
    14701 ttttgaatga agccggttcc cggttctgcc aggaacgtcg gcaacacact cccttccgca
    14761 taccccgaca taaagagtac gcgaaggctg ggccattgct gccggaggcg ttcggccagc
    14821 gcagggcctg tcatctgcgg catgacgacg tccgtgagga gaatatggat gggcgtcttg
    14881 gcggcggcta cgaggcgaag tgcctcctgc ccgctggagg cctcgatcaa cgtgtatcca
    14941 tagtccgaga gggcctgcgt gagcagcatc cggacggccg tttgatcttc gaccaggaga
    15001 atggtttcgt gaccacagtg agggcgcgag ggagtggccg gagcagcggc cggggatgga
    15061 gcctgagcca aggggaaata catgtcgaac gtggcgccgt cgccagggtt gctgtcagcg
    15121 aagataaagc cctgactttg tttgacgatg ccatagaccg tggccagacc gaggccggtt
    15181 cctttgccct cctcctttgt ggtgaagaac ggttcgaaaa tatgcgagag ggtggtccgg
    15241 tccattccct ctccggaatc gcgaatggaa aggtggacat accgacccgg aagcatcgcg
    15301 ggatggggga ccggcgtgtc tgccgtcagg gtcacggttc gggtcgccat cgtgagcgtc
    15361 cccccgtggg gcatcgcgtc cttggcgttc accgccagat tcagcacgac ctgatcgatc
    15421 tgtccccggt ccgcgcggat gatgcagggc tgagacgtga ggtctctcac gagggcaata
    15481 tcttcgccga tcaatcgttc aatcatggag ctgatcgagg agagcgcctc attgagatcg
    15541 aaaacctgga acgtcaggac ctgtcgacgg ctgaaggcca ggagttgctt ggtgagcgcg
    15601 gccgcccgct gaccggcggt cagaatttcg gtcagcgatc gatgcagggg gtcttcggcg
    15661 cgcacctgtt ccaataggag catggcgcac ccgttgatga cggtgagcag attgttgaaa
    15721 tcgtgagcga cgccgccggc caatctgccg atcccctcca tcttatgggc ttgccggagc
    15781 tgttcctcca gttgtttgtg ctccgtgacg tcgatgaaaa agccgatcga cccggtcgtc
    15841 tggccgccgg gaccttgcag caacgttccc cacatgttca catcgatcaa ggtcccgtct
    15901 tttcgctgcc gacagagttc gaggttgcgc ggcgcgccgc cgttcatgac agaggtccac
    15961 aactcgttcg aagcctgttc ctgacccgcg ggcacatagg gaagctcctt gcccagaact
    16021 tcctccctgg tccaaccgaa caaggccgtg gccgcggcat tccaggtcgt gacgtggcct
    16081 gtgtgatcca gctcaatcat tgcgagagga gattcttcga tgatggtctg caaatgcgcg
    16141 gtcatgcgcc gcaattcgcc cgtgcgctcg cggacatgtt gttccagcgc ctcctgcgcc
    16201 tgccggcggt cggctgcttc caaggtgagg gtcgccatgg cggcgagaga ggcggcgaac
    16261 gcttcctcct cactggtcca agtcttgggc gtgccggtat gttcgaggca gaggactccg
    16321 accacacgtc catggcggcg aatgggagcg tccagcatcg aggtgatgcc aagcgggatg
    16381 agatatgaag gggtcaattc gctggtgcgc ggatcagact gtgccgcatg ggcggccaag
    16441 gaatagggct ctgtttcgag ggcgtgaaga taggccggat actgtttagt ggaaagcatg
    16501 gttccctggc tatgccggtt ggaggtcgcc tcgaacaagt cttgtagaac cagggtcgac
    16561 tgggtgccgt cgaagagcca gatgctcgct cgctcaaccg cgaacgtgcc ggccgcgtgt
    16621 tcggtcatga cgggaaacgc ctgttgggga tgcccgctgt g
//
LOCUS       DAOQEW010000076        16396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_947_length_16396_cov_7.533138, whole genome shotgun sequence.
ACCESSION   DAOQEW010000076 DAOQEW010000000
VERSION     DAOQEW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16396
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_16396_cov_7.533138"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..868)
                     /locus_tag="PLS35_09865"
     CDS             complement(53..868)
                     /locus_tag="PLS35_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNI19013.1"
                     /translation="MKRRILLAVGIFATVALSLLPFLWFVLTSFKSQTEIEAAPPAWW
                     PSGSLGFYRSALFDHHLFEYVLNSIVVAGSTTLLALLLAIPAAYALARLTIPGKQGIL
                     AVLLCVSMFPQMAIAGPVWRLLDALGGLNHRWGVVLPYVALTLPLAIWILASFFKELP
                     PELEDAARVDGCGPWGTLFRITLPLATPGIFTAAILILIYAWNEFFFALLILTQPEQQ
                     TLPVGIALFQGEFTMPWGELAAASVVATLPLIGVVLLCQRWIVSGLSAGAVKG"
     gene            complement(865..1776)
                     /locus_tag="PLS35_09870"
     CDS             complement(865..1776)
                     /locus_tag="PLS35_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNI19014.1"
                     /translation="MMRAHAVLRRREGLAAWLMVAPALLVTVVFALYPVADSLWLSLH
                     HIFVGIPQLGSPFVGLENYQALLHDPVAHQAFVVTLGFVVLSTLLELACGLIIALVIH
                     ERFRGRGLVRGAILIPWAIPTVVASQLWRYIFNDQYGFANLLLFGDRVTDYIPWLAYP
                     SVALAVVVLADVWKTSSFAGLLILAGLQVIPGDLYDAARVDGATVWQRFWHITLPLLK
                     PALLLALLFRTMDAFRVFDLVFVMTQGGPGDATQVLQFYGYQTLFTEGRIGYGSAISV
                     AVFLMILALSLTYLRAIGSNLLERRRS"
     gene            complement(1773..3149)
                     /locus_tag="PLS35_09875"
     CDS             complement(1773..3149)
                     /locus_tag="PLS35_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI19015.1"
                     /translation="MRWISSRQRIQRPSQSCAVTVSRWIVLLTLIVLTGSTSLGSAES
                     LPGTTATTPKPRVTLRFVSWKPDHARVWEEVLGDFTHAHPQITVVRELAPHSSTAYHD
                     LLTQKLKNRDATVDVFFIDVIWVPEFAEAGWARPLDDKFSPSMRQDFLPAAVEVGRYD
                     THYYGVPSRIDAGLLYYRQDLLEKYGFAAPTTWPELIQQAETILAGERASNPLLQSYS
                     AQFKQYEGLVCNMLEFVEGHGGSLLRPDGTGSTLSSPRALAAVQFVRDRVIGRLTSRA
                     ALTYQEPEALSLFLQGHAIFHRNWPYAWELANNRTRSTIAGQVGVAPLPGFSPGRTAA
                     ALGGWLYGISAYSPHPDEAWALIEFLSSQAVQKKFSREAGIAPSREVLFSDPEVLAHS
                     PQLRNQRSVLHAATPRPRSPVYPAISHLLQRYFSRALALEDIDLAQEAAVTDAHIDRL
                     LALTKAAP"
     gene            3469..4143
                     /locus_tag="PLS35_09880"
     CDS             3469..4143
                     /locus_tag="PLS35_09880"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNI19016.1"
                     /translation="MNGLQVSAVSPRRGRLLRMTLGGLLGWALILIAITDAWAVQASP
                     VSLSFSAVQGGPNPASQVVTISKSTSRQVGWKAVDDAAWLSTAPSSGSLTSSAQVVVA
                     VNVSGLAAGTYSGSMKVTLSKGGSLSIPVTLTVASATSTKSTTTVTTATLSWAPNTDS
                     DLAGYKVYMGTTSGLYGTPVDVGNVTTYTAGNLTTGTTYYFSVTAYDQSRNESLHSGE
                     VSKSIY"
     gene            complement(4189..4575)
                     /locus_tag="PLS35_09885"
     CDS             complement(4189..4575)
                     /locus_tag="PLS35_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI19017.1"
                     /translation="MKVPFTLGAVWHFGLAVRDPRASAKWFMRVAGLSKQFEYEGGVG
                     IGNANVLIVFSKGKPSPKTVGHVAFHLPTMAALKKALAHLKKLKVQVEDPGDEIGPVA
                     PGSKSMGIWFSDPDGYRWELAVQSKK"
     gene            complement(4588..5178)
                     /gene="ogt"
                     /locus_tag="PLS35_09890"
     CDS             complement(4588..5178)
                     /gene="ogt"
                     /locus_tag="PLS35_09890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF007626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNI19018.1"
                     /translation="MSQQLHLLTETLITPIGTLVIMTDEQGQLRAVDWVDFEDRMRKL
                     LTRHYGLQGVLLTPAVNSTCHAAALSAYFEGNLHAIDALFTATAGTPFQKRVWNALRE
                     IPAGETVSYGDLARRIGQPTAVRAVGLANGANPVSIVVPCHRVIGADGSLTGYGGGLN
                     RKRWLLNHEKEAMAGSARFAAPSPTAAMVGCASFHR"
     gene            complement(5208..6677)
                     /locus_tag="PLS35_09895"
     CDS             complement(5208..6677)
                     /locus_tag="PLS35_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNI19019.1"
                     /translation="MELLDPDISYRALQTRDARFDGRLFVGVISTGIYCRPICPARTP
                     KREHCRFFSGSAAAQAAGFRACLRCRPEIVPELPGWRGTSNTVSRALALIAEGFLDHE
                     DGTVEALAMRVGMGARQLRRLFDQHLGVAPVAVAQSRRLLFAKQLIHETKLSMSDVAL
                     AGGFGSVRRFNHAFRSLYHRAPGELRRDTTAASVTLAATAPVMLQIPYRPPYDWDSML
                     AYLRARAIEGLEVVEENAYRRSVLHEGLTGTITIEHRPDKHSLMATIDFPSVRALQAM
                     VARIRHQFDVAADIEAIAAHLSKDVTLAPLIAARPGLRAPGCWDGFELAVRAVLGQQV
                     TVAAARRLGGTLVGLWGEPLPHAVDRRLMRTFPTARCLATADLSRLGMPAARRATISA
                     LARTAISYPQLFMPGGSVEERLAQLRAIPGIGTWTAHYIALRAFREPDTFPATDLGLI
                     RSARRLIGEQTSASHLLERTEAWRPWRAYAAQYLWTADQ"
     gene            complement(6788..7738)
                     /locus_tag="PLS35_09900"
     CDS             complement(6788..7738)
                     /locus_tag="PLS35_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19020.1"
                     /translation="MNCTKCPTSTRAVIGLPRHNAAFCKACFTTFVHEQVARAIKAFK
                     MFTPEDRILVAVSGGKDSLALWHILLALGYQADALYVNLGIGAYSEESHRKVRHYADT
                     VAASHGAKLLVHVVEQEAGAGIRELATILHRPTCSTCGTIKRYQFNRAAVEQEYDVMA
                     TGHNLDDEAARLLGNVLHWQNDYLDKQSPTLPASVEGFAKKVKPLCRLSEREIAAYAV
                     VNRIDYIVEECPMAKGSKMILYKEVLNRLETESPGTKQRFYWGFLDKQTAPAPATESM
                     AEKDQRTLAPCDTCGQPTTAGTCSYCKMMAKAKTAAQPRA"
     gene            complement(7838..8035)
                     /locus_tag="PLS35_09905"
     CDS             complement(7838..8035)
                     /locus_tag="PLS35_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNI19021.1"
                     /translation="MLVQLSHPHRQVEIKGPKRTKELLRELNLVMEAHLVIRGDELVT
                     EDEMLADADQIEIRPVISGGT"
     gene            complement(8169..8324)
                     /locus_tag="PLS35_09910"
     CDS             complement(8169..8324)
                     /locus_tag="PLS35_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19022.1"
                     /translation="MVNEQNLDWKNSSFSAVDVNNQKYKRLALASAMLFLGVVALLIY
                     QIIQNIK"
     gene            complement(8591..9706)
                     /locus_tag="PLS35_09915"
     CDS             complement(8591..9706)
                     /locus_tag="PLS35_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19023.1"
                     /translation="MRRASFPLISIGLGILLALLTMELVLRFLPVNEGARPQVVDHEH
                     PVRSLEPNRTFVWSADWNFAIVNVARTNNLGFVNDQDYLLDQPSPLLALIGDSYVEAL
                     MVPFRESVAGRLMTRSPSTERVYSFGTSSSALSNYLAYADYAAKQFHPQAMVFVIIGN
                     DFDESLLKYKNEPGHSYFVEDAHGTLALQRIDYHPSLWRRIIRASALVRYVTLNTDLL
                     NFVLKWQHLPFGRPSNHLAFVGNTGSKADPVRIADAQRAIDTFLTRVAEASGLPPHRI
                     LFAVDGMRPHIYNATTWQLTTGSYFDIMRQYFMEHARQQGFEVLDLQPRFAEHFAQHR
                     RPLEFATDNHWNSLGHEIVEEAIAHSDLYHWFRELAP"
     gene            complement(9802..10803)
                     /gene="galT"
                     /locus_tag="PLS35_09920"
     CDS             complement(9802..10803)
                     /gene="galT"
                     /locus_tag="PLS35_09920"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNI19024.1"
                     /translation="MPELRRDPIVGRWVIISTERSGRPQDVHMPSAPLPSAALCPFCP
                     GQERLTPREILAYRPHASEPDGPNWTVRVIPNKFPALQVEGDMGREGLGLYDRMNGIG
                     AHEVIIETPAHKESLGDLPAKRVEDVLWAYRDRILDLKKDLRLRYILIFKNHGAAAGA
                     TLEHSHSQLIALPVVPTSVLEEIDGCKQHFQQKERCIYCDIMRQESSEGTRVVLENPE
                     FLCLTPYAPRFPFEMWILPKRHAGYFEECQRTQFEFLAPMLGEALRRMDAVLARPAYN
                     FILHSSPLHEKTGDFYHWHIEIIPKLTQVAGFEWGTGFYINPVAPEEAAKCLRDAVL"
     gene            complement(10905..13109)
                     /locus_tag="PLS35_09925"
     CDS             complement(10905..13109)
                     /locus_tag="PLS35_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNI19025.1"
                     /translation="MKTIQLCFLWHMHQPYYTDPLTGSASMPWVRLHATKAYYDMAFL
                     LEHFPEARSTFNFTPSLLLQLEEFSAGRVRDLFLEHAQRPAADLTPTEKAFLIRHFFS
                     ANWATMVRPFPRYQELLVKRGIDVHGQDLERLARQFSTQELLDLQVWHNLAWFGYGSL
                     QRFPRLAELRAKNRAFTEEDKQEVLALQQTAIRQIVPMYKALQDRAQIELTTTPFFHP
                     ILPLVIDSEFTRRARPDLPLPARFHAPADAEAQVRRAVEYHTRTFGRAPAGLWPSEGS
                     VCPEVLPIVAQAGIRWLATDEGILYRSLQMAGQGWNRHYHLYQPYAVGTETHPLAMVF
                     RDRDISDAFGFVYHKTTPEAAADDVLRRIRSLAYDIRLDNGTIAVILDGENPWEHYHD
                     GGERFLSLLFRAFESDGLQLGNGIRVTLNTMSDAMKAAPPTQRLEQLHSGSWINQDFK
                     IWIGHQEDNRGWDLLQHTRARLVELTPSLEPDKARAAWDELYAAEGSDWFWWYGDDFD
                     TDYKQEFDRLFRTHLRNVWTYAGMTPPEILNQPLVEARTPQGLDLVQLPLALISPTLD
                     GLASNFFEWRGAGTINPTPPLGAMWKSEGLFTAIFFGFDREQLYLRLDFDERSDTRQE
                     QCSADLYIGSGIQQHRLSFALKAGAADHFQLARADESGTYRDRGSYSTICRQKILELG
                     IPFKDLDIEVGAELRLTLTVSEHGMEIARYPHHSPATFTRPDDDFEATMWRV"
     gene            13260..13697
                     /locus_tag="PLS35_09930"
     CDS             13260..13697
                     /locus_tag="PLS35_09930"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNI19026.1"
                     /translation="MASEQMVSTEIRGGSLVTPLSIMRYWGPVGLYAGLIFFGSSIST
                     PPEAISSLIKDISDKVLHFSEYALFGALIYRAARHGSGPWMAQHAVWAAVAGSALYGV
                     SDETHQLFVPFRQSDVLDVIADTVGGTVGAWGWRWIEQRTYRD"
     gene            complement(14260..15231)
                     /locus_tag="PLS35_09935"
     CDS             complement(14260..15231)
                     /locus_tag="PLS35_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI19027.1"
                     /translation="MQYVHLGRSGLRVSRLCLGTMNFGPHTTEADSGQIMDRALELGI
                     NFFDSANVYGWKVGEGVTEQIVGRWLAQGGGRREKVVLATKVYGRMGDWPNESRLSAR
                     HIKQACESSLRRLNTDYIDLYQMHHIDRDCPWEEIWQAMEQLCREGKVLYIGSSNFAG
                     WHIAQAQELAKSRHFLGLISEQSLYNLLERTVELEVIPACQAYGIGIIPWSPLARGLL
                     GGALSSPTSGRRAEEDVRKDIETHRQKLEAYEQFCRTLNTAPATVALAWLLHQPAVTA
                     PIIGPRTMEQLTAAISSLELTLTTEHLKTLDAMFPGPGGAAPEAYAW"
     gene            15385..>16396
                     /locus_tag="PLS35_09940"
     CDS             15385..>16396
                     /locus_tag="PLS35_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNI19028.1"
                     /translation="MSTTNPAAPAAPLTPPDPERIAQTVATRLHFRGEMTALSALAGD
                     ASNRRYFRIFLKGTPSGLILMQLADPEGFKKSEEAVSGASAQITELPFINVLTHLQRT
                     GVTVPTLHYFDREAGLLYLEDFGDLTLAEACRGADPARLEALYRQAIDQLVLLQVKGT
                     TPPAPGCIAFHRRFDVPLLMWEFDHFLEYGITARLGQPMKPEDDSAVRAAFQRIAELL
                     AGQPQVFVHRDYHSRNLMVDGSRMGIIDFQDALMGPATYDLASLLKDAYIELDDAVVD
                     RLVEHFLDGLAGLGVKVNDRSAFRRVFDLTSIQRNLKAAGRFVYIDRVKHNPKFLADI
                     PRV"
BASE COUNT         3308 a         4571 c         5153 g         3364 t
ORIGIN      
        1 tgaggatgtt ttcaagccga gaacaaagct ggaggccttt ttcaacatcc tgtcatcctt
       61 tgaccgcgcc ggcggacaac ccgctcacaa tccatcgttg gcacaacaac accacgccga
      121 tcaacggcag agtggcgact accgacgctg cggccaattc tccccaaggc atggtgaatt
      181 ctccctggaa gagcgcgatg ccgacgggca atgtctgttg ctccggctgg gtcagaatca
      241 agagcgcaaa aaagaattcg ttccaggcat aaatcagaat caaaattgca gccgtaaaaa
      301 tccccggcgt cgccaacggc agcgtgatcc ggaacagggt gccccaaggc ccgcagccat
      361 ccactcgcgc cgcatcctcg agctccggcg ggagctcctt gaagaagctg gccaggatcc
      421 agatcgccag tggcaaggtc agcgcaacgt aaggcaacac cacgccccaa cggtggttga
      481 gtccgcctag tgcatcgagc agccgccaga ccggtccggc aatggccatt tgcgggaaca
      541 tcgatacgca gagcagcacg gccagaatac cttgtttgcc tggaatggta agccgggcca
      601 gcgcatacgc ggccgggatc gcgaggagaa gggccaggag cgtggtagaa ccggccacca
      661 cgatgctgtt cagtacgtac tcgaacaggt gatggtcgaa gagcgcgctg cgatagaaac
      721 ccagactgcc tgagggccac caggcaggtg gtgctgcttc gatttcggtt tgtgacttga
      781 acgaggtgag gacaaaccag aggaacggca acagactcag cgcgacggta gcgaagatgc
      841 cgaccgcaag caggattcgg cgtttcatga acgtctgcgc tccaggagat tcgatcctat
      901 ggcgcggagg tacgtcaacg acagcgccag gatcatcagg aacacagcca cggagatggc
      961 agacccatac ccgatgcggc cttccgtaaa taacgtttga tagccataaa actgcagcac
     1021 ctgggtggcg tcgcctgggc cgccttgcgt catcacaaac accagatcga agacacgaaa
     1081 ggcatccatg gtgcgaaaca ggagcgcgag caacagcgcc ggtttgagga gcggcagggt
     1141 gatgtgccag aaccgctgcc acactgtggc gccgtcgacg cgtgcggcat catagagatc
     1201 gccgggaatg acttgcagac cggctagaat caggaggccg gcgaaggagg aggttttcca
     1261 cacgtcggcg aggacgacga cggccaaggc cacgctcgga taggccagcc aggggatata
     1321 gtcggtgacg cggtcgccga acaggagcag attggcgaat ccgtactggt cgttgaagat
     1381 gtagcgccag agttgcgagg caacgactgt tggaatcgcc cagggaatca ggatcgcgcc
     1441 gcgtaccagg cctcgtccac gaaatcgttc atgaatgacg agggcaatga tcaggccgca
     1501 ggctaactcg agcagggtag aaaggactac aaacccgaga gtcacgacga acgcttgatg
     1561 cgcgaccggg tcatggagca gcgcctggta attctccaag cccacgaatg gactgccaag
     1621 ttggggaatg ccgacgaaga tgtgatgcag gctgagccag agcgaatccg cgacgggata
     1681 taacgcaaag accactgtga ccaggagtgc cggtgccacc atcaaccagg cggccaggcc
     1741 ctctcggcgt cgaagtacgg catgcgcacg catcatggcg ccgctttcgt caatgccaga
     1801 agtcgatcga tgtgcgcatc ggtgacggca gcttcttgcg cgaggtcgat gtcctccagc
     1861 gccagggcac ggctgaagta gcgctgcaag agatgtgaga tggcgggata caccggcgaa
     1921 cgcggacgag gcgtggccgc gtggaggacg ctacgctgat ttctgagctg cggcgaatgg
     1981 gccaggacct cagggtcgga gaagagcact tcccgggacg gcgcgatgcc ggcttcacgc
     2041 gagaatttct tttggacagc ctggctggag agaaattcaa tgagagccca ggcttcgtcc
     2101 ggatgtggcg aataggcgct gatgccgtac agccagccac cgagtgcggc cgccgtccgt
     2161 cctggcgaga atcccggtag aggcgccacg cctacctgtc cggcaatggt cgaccgcgtc
     2221 cggttattcg cgagttccca ggcatagggc caattccgat gaaagatcgc gtgtccttgc
     2281 agaaataggg acagcgcttc cggttcctga taggtcaggg cggcacggga cgtgagccgg
     2341 ccgatgactc gatcacggac gaattgcacg gcggccagtg cgcgaggcga cgagagggtg
     2401 gacccggtcc catccggtcg caacagactt ccgccatgcc cctcgacaaa ttccagcatg
     2461 ttacagacca agccttcgta ctgcttgaat tgcgccgagt agctttgaag cagcgggttg
     2521 ctcgctcgtt cgcccgccag gatggtttct gcctgctgga tgagttccgg ccaggtcgtc
     2581 ggcgcggcaa atccgtattt ttcgagaagg tcctggcgat agtagagcag cccggcatcg
     2641 atgcggctcg ggacaccgta gtaatgggtg tcataccggc cgacctcgac ggcggcaggc
     2701 agaaagtctt gtcgcatgga gggggaaaat ttatcgtcga gcgggcgggc ccacccggcc
     2761 tctgcaaact ccgggaccca gatcacgtcg ataaaaaaga catcgacagt ggcatcgcgg
     2821 ttcttcaact tctgcgtcag gagatcgtga tacgccgtcg agctgtgcgg ggccagttca
     2881 cgcactaccg tgatctgggg atgtgcgtgg gtgaagtctc cgagcacctc ctcccacacg
     2941 cgtgcatgat cgggtttcca ggagacgaag cgaagcgtga cgcggggctt gggagtggtg
     3001 gcggtagtcc cgggcagtga ttccgcgctc ccgaggcttg tactgccggt gagtacaatg
     3061 agtgtgagca ggacgatcca gcgggatacc gtgacggcac acgactgtga aggacgctgg
     3121 atgcgctgac gtgaagatat ccatcgcatc actctgtcag gttatacaca gtccggtggg
     3181 gtgatgcaaa accaccaggc cgcagaaggg tgcctgtgcc aactgcggca gactgcacaa
     3241 tttggggctc tcaagaagtg tcacgcctac accggcatgg ggcgtcactg agatggtcga
     3301 gaaaaaccgt tgtgaagacg gaggcttgcg aaggtgctcc ttctggcatg accactgcta
     3361 ttgtttgtag aggggaagtg gccttgccga cgtttcgaca gatcgcagag agagcagttc
     3421 aatcagaggc ccgccgattc tcgcgggcgc tggtgtgaag gaggcaatat gaatggcctg
     3481 caagtatcag cggtttcgcc caggcggggt cgcctgctgc ggatgactct tggtgggtta
     3541 ctgggatggg ctctcatcct gattgccatc acagatgcct gggctgttca agcgagtccg
     3601 gtcagcctca gctttagtgc cgtgcagggc gggccgaatc cggcgagcca ggtcgtgacc
     3661 atctccaaga gcacatcgcg tcaggtcgga tggaaggccg ttgacgatgc tgcgtggttg
     3721 agtacggctc cttcctcagg aagcctaacc agttcagcgc aggtcgtggt ggcggtcaat
     3781 gtaagtggat tggctgccgg tacctatagc ggctcgatga aggtgacatt gagtaaaggg
     3841 ggaagtttgt ccatccccgt caccctgacg gtcgcgtcgg caacgtccac aaaatcgacg
     3901 acgaccgtca cgacggcaac cctttcctgg gcgcccaata cggatagtga cctcgccggg
     3961 tacaaggtgt atatgggcac gacctccggt ctgtacggta cgccggtaga cgtcgggaat
     4021 gtgaccacct ataccgccgg caacttaaca actggaacca cgtattactt ttccgtgact
     4081 gcgtatgacc aaagccggaa tgaaagcctc cactcaggcg aagtgagcaa gagcatctat
     4141 tgatctcgtg ctccttcccg agttgccatt accggctaag tacacgtctt atttcttgct
     4201 ctgcacggcc aattcccacc ggtatccgtc ggggtccgag aaccagatcc ccatgctctt
     4261 ggatcccggc gccacgggcc cgatttcatc tcccggatcc tcgacctgaa ccttcagctt
     4321 tttcagatgg gccagggctt ttttgagtgc ggccatcgtg ggtagatgga atgccacatg
     4381 gccgacggtt ttcggcgatg gttttccttt ggagaagacg atcagcacat tggcgtttcc
     4441 aattcccacg ccgccttcat attcaaactg tttgctgagc cccgctaccc gcatgaacca
     4501 cttggcgctt gcgcgcgggt cacgaaccgc cagcccgaaa tgccatactg cgccgagcgt
     4561 aaatggaacc ttcatatccg tttcctccta acggtgaaat gaggcacatc ctaccatggc
     4621 ggcggtgggg cttggcgctg caaaacgcgc ggagcccgcc atcgcctcct tctcatggtt
     4681 gagcagccac cgtttgcggt tgagtccacc gccgtagcca gtgagggagc cgtcggcgcc
     4741 gattacccgg tggcagggaa cgacgatgct gacggggttc gcgccattgg cgagcccgac
     4801 tgcgcgcacc gccgtcggtt gcccgatgcg tctcgccagg tcaccgtaac tcaccgtttc
     4861 tcctgcggga atttcgcgca gcgcgttcca gacgcgtttc tgaaacgggg tgcctgccgt
     4921 cgcggtgaag agcgcgtcga tggcgtggag attgccctcg aagtacgccg acagggccgc
     4981 cgcatggcac gtggaattga cggccggcgt caggagcacg ccctgtaaac catagtggcg
     5041 cgtcaacagt ttccgcatgc gatcctcgaa atcaacccaa tcgaccgccc gcaactgccc
     5101 ctgttcgtct gtcattatca ctaaggttcc aatcggcgtg atcaaggttt ctgtcaaaag
     5161 gtgtagttgt tgagacatga tacatctccg tgcaggtggt gacagggcta ttgatcggcg
     5221 gtccagaggt attgtgcggc gtaggcgcgc catggtcgcc aggcctccgt gcgctccaac
     5281 agatgagagg cactggtctg ttctcctatc aggcgcctgg cgctccgtat cagaccgagg
     5341 tcggtggccg ggaaggtgtc cggttcacgg aaggcacgca gggcgatata gtgtgccgtc
     5401 cacgttccga ttccgggaat cgcccggagt tgcgcgagac gctcttcgac tgatccacca
     5461 ggcatgaata gttgtggata agagattgcc gttcgtgcaa gagcgctgat cgttgctcgc
     5521 cgcgcggcag gcatgccgag ccggctcaag tcggcggtcg cgagacagcg ggccgtcgga
     5581 aacgtgcgca taaggcgcct gtccactgcg tgtggtaagg gttctcccca cagcccgacc
     5641 aatgttccgc ccagccgccg tgcggccgcg accgtaacct gttgtcccag caccgcgcgg
     5701 acagccagtt caaaaccgtc ccaacagcca ggggcgcgca gtcccggtcg cgcggcgatg
     5761 aggggcgcca gggtcacatc ctttgagaga tgggctgcga tcgcctcgat gtcggctgcc
     5821 acgtcgaatt gatgccggat gcgcgcgacc atggcctgga gggcgcggac cgatggaaaa
     5881 tctatcgtgg ccatcaggct gtgcttgtcc ggtctgtgct cgatggtgat agtgcccgtc
     5941 agcccttcat ggagcacgct acggcggtag gcattctctt cgacaacctc caggccttcg
     6001 atggcgcgcg cccgtaagta tgccaacatg ctgtcccagt cgtacggtgg ccgataagga
     6061 atctgcagca tgacaggtgc agtggcggcg agggttacgc tcgccgccgt ggtgtcacgg
     6121 cgcaattcgc ccggtgctcg atgatagagg gagcggaacg catgattgaa ccggcggaca
     6181 ctgccgaatc ctcccgcaag ggccacatcc gacatcgaga gtttcgtctc gtggatcaac
     6241 tgcttcgcaa acagcaggcg tctggactgg gccacggcta ccggcgcgac gcccaggtgt
     6301 tgatcgaaga gccggcgtag ctgccgtgct cccataccga cgcgcatcgc gagcgcctcg
     6361 accgtgccgt cctcatgatc gagaaatccc tcggcaatca gcgccagcgc tcgggagacg
     6421 gtgtttgacg tgccgcgcca cccgggcagt tcgggaacaa tttccgggcg acaacgcagg
     6481 caggcgcgga agccggctgc ttgtgccgcc gcactgccgg aaaaaaaccg acagtgttcc
     6541 cgttttggcg tgcgagccgg acagatcggc cggcaataga tcccggtcga gatgacgccg
     6601 acgaagaggc ggccgtcaaa tcgggcatca cgggtctgca gcgcgcggta gctgatatcg
     6661 ggatcgagca gttccatgcc cacactctgc catattcaac ggagaaattc cggccgtttt
     6721 cggacgtgac cattcgattg cggaggtcac aacaaaaatc gtgggaggcg cgtcgtcggt
     6781 tggatggtca ggcgcgaggt tgtgcggcgg ttttggcctt tgccatcatc ttgcagtagg
     6841 agcaggtgcc ggcggtagtg ggctggccgc aggtgtcgca gggcgcgagc gtgcgttgat
     6901 ctttctcggc catggattcg gtcgcggggg ctggtgcggt ttgtttgtcc agaaaccccc
     6961 agtagaaacg ctgcttcgta ccgggggatt ctgtttccag ccggttgagc acttccttgt
     7021 agagaatcat tttcgagccc ttggccatgg ggcattcttc gacaatgtag tcgatccggt
     7081 tgaccacggc ataggcggcg atttcccgtt cggacaaccg gcagaggggc ttgacctttt
     7141 tcgcaaatcc ttccaccgat gccggtaggg tggggctttg cttatcgaga tagtcgttct
     7201 gccagtgcag gacattcccc agcagccgtg ctgcttcgtc gtcgaggtta tggccggtgg
     7261 ccatcacgtc atactcctgc tccactgccg cgcgattgaa ttgatagcgc ttgatcgttc
     7321 cgcaggttga gcaggtcgga cggtgaagga tggtcgcgag ttcacgaatg ccggcccccg
     7381 cttcctgctc gacgacatgc accagcagtt ttgcgccatg ggaggcggcc accgtgtcgg
     7441 catagtgccg cactttccgg tgagattctt ccgaataggc cccgatgccg agattcacgt
     7501 agagcgcgtc agcctggtat ccgagtgcga ggagaatatg ccagagggcg agactgtcct
     7561 tgccgccgga aaccgccacg agaatccggt cttcaggtgt aaacattttg aaggccttaa
     7621 tggcacgcgc cacctgttcg tggacaaagg tcgtaaagca ggccttgcag aaggcggcat
     7681 tgtgccgcgg caatccgatt accgcgcgtg tactggtcgg gcacttcgtg caattcatgg
     7741 tgttccaggt gttggcagtc agtcgtcagg tttcagcaag cgggcgaagc cggtcgacga
     7801 tggctcgaga aatgccggtc gatcacggat cgttgagcta tgtcccgccg gagatcaccg
     7861 gccggatttc aatctggtcg gcgtcggcaa gcatttcatc ttccgtcacg agttcatcgc
     7921 cccggatgac caaatgcgct tccatgacca gattgagttc gcgcagcagt tccttggtgc
     7981 gcttgggccc cttaatttca acttgtctgt gagggtgact caactgaacg agcatgcgcg
     8041 atgatacggc tgatacgcgc gggattgcaa tcggatcatg atccgtcgga gtcaggcgga
     8101 gaagtgtggt cgcgcgaggc cgccagaagc ccgcttgcgg ctcattcctg acgcgctaga
     8161 ggagagcatt acttaatgtt ctgaatgatc tggtagatca gcaatgcgac cacgccaagg
     8221 aacaacatcg cggaggccaa ggccagacgt ttatatttct ggttgttgac gtccactgca
     8281 ctgaatgagg aatttttcca atcaagattt tgttcgttca ccatgtcctc ctacctctac
     8341 cgtcccggta ttctttgcct ccccatcccg atcctcggga tgcatatatg cattcactga
     8401 gtactgaagc cgaaggccgt cccgttccac ccgaggccct agggatagca ccaggtttgt
     8461 atacgctcgc atgcctggga attgcaagga aagctactag acaaaattct ggtaacgacc
     8521 gagttttatc taatcgcccg agcgagcgtg ctggagacat cactgactgt gccgtacaag
     8581 aaatcgtccg ctagggggcg agttcccgaa accagtgata cagatcggaa tgcgcgatgg
     8641 cctcttcgac gatctcatgc cccagcgagt tccagtggtt gtcggttgcg aattcgagtg
     8701 gacggcggtg ttgggcaaaa tgctcggcga atcggggttg caggtcgagg acttcaaacc
     8761 cctgttggcg ggcgtgctcc ataaaatact gtcgcatgat atcgaaatag gacccggtgg
     8821 tgagctgcca cgtggtggcg ttgtagatat gcgggcgcat accgtcgacg gcgaagagga
     8881 tccggtgtgg cggcagaccg gatgcttcgg ccacccgcgt caagaacgtg tcgatcgctc
     8941 gttgggcatc ggcaattcgc acggggtcgg ctttggaccc ggtgtttccg acaaatgcga
     9001 ggtggttcga tgggcggcca aatgggaggt gttgccactt cagcacgaag ttgaggaggt
     9061 ccgtgttcag cgtgacatag cgcaccaggg cggacgcgcg aatgatgcgg cgccacaggg
     9121 aggggtgata gtcgattcgt tgcagcgcca gggttccatg cgcatcttcc acaaaatacg
     9181 agtgccccgg ttcattcttg tatttcaaca gactctcatc gaaatcattg ccgatgatga
     9241 cgaagaccat tgcctgggga tgaaactgct tcgccgcata atccgcatag gccaggtagt
     9301 tgctcagagc tgagctggat gtgccgaagg aatagacgcg ttcagttgag ggggagcggg
     9361 tcatcagtcg tccggcgacg ctctcccgaa acggcaccat caacgcttcg acataactgt
     9421 cgccgatcag ggccaggagc ggcgacggct ggtcgagtag ataatcctgg tcgttgacga
     9481 agccgaggtt gttggtgcgg gcgacattga cgatggcgaa attccagtcg gcagaccaga
     9541 caaacgtccg gttcggctcc agagaacgga cgggatgctc gtgatcgacg acctgggggc
     9601 gcgctccctc gttgaccggc aggaaccgca gcaccagttc catggtgagt agtgccagca
     9661 gaatccccaa gccgatactg atgagcggga aactggcccg tctcataccg gacgggcacc
     9721 aagctggaga ggaagagatg aaaactcttc agcgggccct gcggaacggg gggctagcgg
     9781 tatgcctgcg atgaagccca attatagaac ggcatccctg aggcatttcg ccgcctcttc
     9841 cggtgccacc ggattaatgt agaaaccggt gccccactcg aatcccgcca cctgcgtaag
     9901 ttttgggatg atctcgatgt gccagtgata gaaatctccc gtcttttcat ggagtggcga
     9961 gctgtgcagg atgaagttat acgccggcct ggccaggacg gcgtccatgc gccggagggc
    10021 ttccccgagc ataggggcca gaaattcgaa ttgggtccgt tggcactcct cgaagtagcc
    10081 ggcgtggcgc ttcggaagaa tccacatttc aaagggaaat ctcggggcat agggtgtcag
    10141 gcacaggaat tccggattct ccaggaccac gcgtgtgcct tccgaggatt cctgccgcat
    10201 gatatcgcag tagatgcagc gttctttttg ctggaagtgc tgtttgcagc catcgatctc
    10261 ttcgaggacg ctggtcggca cgaccggcaa ggcgatcagt tgagaatggc tgtgttccag
    10321 cgtggcaccg gcggcggccc catggttctt gaagatcagg atgtatcgga gccggagatc
    10381 cttcttcagg tcaagaatcc ggtcgcgata ggcccagagc acatcctcca cccgtttcgc
    10441 ggggaggtcg cccaggcttt ctttatgagc gggggtttcg atgatgactt cgtgggcgcc
    10501 gattccgttc atgcggtcat acaagccgag gccttcccgg cccatgtctc cttcgacctg
    10561 cagtgccggg aatttattgg gtatgactcg cacggtccag ttcgggccgt ccggttcaga
    10621 ggcatggggc cggtacgcca ggatctctct gggcgtcagc cgttcctgtc ccgggcaaaa
    10681 cggacagagg gcggctgagg gaagcggcgc cgacggcata tggacatcct gagggcgtcc
    10741 gctccgttca gtcgaaataa tcacccagcg gccgacgatc gggtctcttc tcaattcggg
    10801 catctcaact cctgtggttc aaatcaattg ggcgtgatgc tcattgcgta aaacgggcat
    10861 cgatccgagt ggtagcgtga tccgcgagcc gcgcaccgtt ccgttcagac tcgccacatc
    10921 gtggcttcaa agtcgtcgtc cggccgagta aaggtggccg ggctgtgatg aggataccgc
    10981 gcgatttcca tgccgtgttc cgacacggtc agcgtcagcc gcaattctgc gccgacctcg
    11041 atgtccaggt ctttgaacgg aatgcctaat tcgagaatct tttgccggca gattgtgctg
    11101 tacgaacctc tgtcgcgata ggtgccggat tcgtccgccc gtgcgagttg aaaatgatcc
    11161 gccgcgccgg ccttcaaggc gaaggacagt ctgtgttgtt ggatgcccga accgatgtac
    11221 agatctgccg agcattgctc ctggcgggtg tctgatcgct catcgaaatc gaggcggagg
    11281 tacagctgct cgcgatcgaa tccgaagaaa atggcggtga acagcccctc ggatttccac
    11341 atcgctccca gcggcggtgt gggattgatg gtgcccgcgc cgcgccattc gaaaaagttc
    11401 gatgccaagc cgtccagagt cgggctgata agggccagtg gcaattgcac gaggtcgagg
    11461 ccctgcggtg tgcgcgcttc gaccaaaggc tgattcaaga tctcgggcgg cgtcatcccg
    11521 gcgtaggtcc agacgttgcg caggtgcgtg cgaaacaggc ggtcgaactc ctgtttgtag
    11581 tcggtatcga aatcatcgcc ataccaccag aaccaatcgc tgccctcggc ggcatacaat
    11641 tcgtcccagg cggcgcgggc cttatcgggc tccagtgatg gcgtcagttc gacgaggcgt
    11701 gcccgtgtgt gctgcaggag atcccaaccg cgattgtctt cctggtggcc gatccagatt
    11761 ttgaagtcct gattgatcca ggatccggag tgcagctgct cgagtcgctg ggtcggcggc
    11821 gcggccttca tggcgtcact catggtgttc aaggtcacac gaatgccatt gcccaattgg
    11881 agcccatccg attcgaaggc gcgaaagagc agggagagaa accgttcgcc gccgtcgtgg
    11941 taatgctccc agggattttc gccgtcgagg atcaccgcga tggttccgtt gtccagccgg
    12001 atatcatagg ccaggctgcg gatccgccgg agcacgtcgt ccgcggcggc ctccggagtc
    12061 gttttgtggt agacgaaccc gaatgcgtcg gagatgtccc gatcacggaa caccatggcg
    12121 agggggtgtg tctcggtccc gaccgcatag ggttggtaga gatgatagtg gcggttccac
    12181 ccttggccag ccatctgcag cgagcgatag aggatgccct catccgtggc cagccagcgg
    12241 atgccggctt gtgccacgat cggtaacacc tccgggcaaa ccgatccttc ggacggccag
    12301 agcccagccg gagcgcggcc gaacgtgcgt gtatggtact ccacggcgcg cctgacttgg
    12361 gcctcagcat cggccggtgc atgaaaccgg gctggaagcg gcaggtcggg tctggcgcga
    12421 cgcgtgaact ccgagtcgat gaccagtgga aggatcgggt gaaaaaacgg ggtagtggtc
    12481 agttcgatct gtgcgcgatc ctgaagcgcc ttatacatgg gcacaatctg acggatggcc
    12541 gtttgctgta aggccaacac ttcctgtttg tcctcttcgg taaacgcacg attcttcgcg
    12601 cgcaattctg ccaggcgggg gaatcgttgc agactgccgt atccgaacca ggcgaggttg
    12661 tgccagacct gaagatcgag gagctcctga gtggaaaact ggcgggccag ccgttccagg
    12721 tcctgtccgt ggacatcgat gccccgtttg accagcagct cctggtagcg aggaaacggt
    12781 cggaccatcg tggcccagtt cgctgaaaaa aaatggcgga tgaggaaggc tttttccgtc
    12841 ggcgtgaggt ccgccgccgg acgttgtgca tgttccagga acaggtcacg gacgcgtccc
    12901 gcagaaaatt cttcgagttg caacagcaag gacggcgtga agttgaaggt cgaacgggct
    12961 tctggaaagt gttcgagcag gaaggccatg tcgtaatagg ccttcgtggc atgcaagcgg
    13021 acccagggca tactggcgga ccccgtgagc gggtccgtgt aatacggctg gtgcatatgc
    13081 cagaggaaac agagttgtat ggttttcatc gggaagtttc ttgcagactc acgggcagta
    13141 tgtcataggg gtatgagcgt tgcaatgaaa cggccggatt gatggcacac gctgacttct
    13201 tatggcagtt tggttgtctt gtgcgtgata ggagtggttg aagcggagaa tggacgacaa
    13261 tggcatctga gcagatggtc tcgacagaga tacgaggagg gtcgctggtc acgcccctct
    13321 cgatcatgcg gtattgggga ccggttgggc tatatgcggg cctcatcttt ttcgggtcct
    13381 cgatctcgac gccgcccgaa gcgatttcat cgttgatcaa agatatttcc gacaaggtgc
    13441 tgcatttcag cgaatatgcg ttgttcggag ccctgatcta tcgtgccgct cgccacggat
    13501 ccggtccatg gatggcgcaa catgcggtct gggcggcagt ggccggttca gcgctgtacg
    13561 gcgtgagtga tgagacgcat cagttatttg ttccgtttcg tcaatcggat gtgttggacg
    13621 tgattgcgga cacagtgggt ggtaccgtgg gggcatgggg ctggcgctgg atcgaacagc
    13681 gtacgtaccg cgattaagtt cctagtacga tcatttcatc atccaatccc ttccgtgcgg
    13741 atccctgcct ggcgtgaccc tggtgcctga tcactctctg cgagtcgtat cccgcgcgtc
    13801 ccgcctcttc gatagcccca caccgcacgc agcgcatcgc acgaatccag acatggcctc
    13861 cggcatcgac cctgtcaatg tacagatcgg cgacgaggtg tccttgacag tgtgtacaga
    13921 agagaccgga ggacttcgtg gtggaagcag tggcgatccc tgcgcctgtc tgtgtttgga
    13981 tgaggtgtgg cgacatgggt cctcctgctc gtgatgatgg tgggtggtat acctctatcg
    14041 gtacgcaaag aagatgcctg gagcacgcgg ggtggagtga cactgaggga tattgttggg
    14101 gggaaattat cctggtgagt aacggtgctg ctgacgcgga tgacgagcgt cgtggtgacg
    14161 cattgaagcg acttggcgtt gttcgagcgg tcggggggag agtggctggt cggaacaata
    14221 cgcggcaatt ttttccgctg ttgctgcctc aatcaatact taccaggcgt aggcttccgg
    14281 agctgcgcct ccggggccgg gaaacattgc atctaacgtc ttgaggtgtt ctgtcgtcag
    14341 cgtcagctca agactggaaa tcgcggcagt caattgctcc atcgtgcggg ggccgataat
    14401 gggggccgtc accgctggtt gatgcagcag ccaggcgagg gcgacggtgg ccggcgcggt
    14461 attgagcgtc cggcaaaact gctcgtacgc ttctaacttc tgacgatggg tctcgatatc
    14521 tttgcgaacg tcctcctcag cccgccgacc gctcgtgggt gagctgaggg cgccgcccag
    14581 caatccccgt gcgagaggac tccagggaat gatgccgatg ccgtaggcct ggcaggcggg
    14641 aatcacctcg agctcgaccg tgcgctcgag caaattgtac aggctctgct ccgaaatgag
    14701 tcccaggaag tggcgtgact ttgccagctc ttgggcctga gcgatgtgcc agccggcaaa
    14761 attgctgctg ccgatgtaga gcaccttgcc ttcgcggcag agttgctcca tcgcttgcca
    14821 gatttcctcc caggggcagt cacgatcaat atggtgcatc tggtaaaggt cgatgtagtc
    14881 ggtgttcagg cgccgcagac tgctttcaca cgcttgtttg atatgccgcg ccgagaggcg
    14941 tgactcgttg ggccagtcgc ccatccgtcc gtacaccttg gtggccagca cgaccttctc
    15001 gcgccgaccg cctccctgag ccagccatcg gccgacgatc tgttccgtga ccccttctcc
    15061 caccttccag ccatacacat tggcgctgtc gaagaaattg atgcccaact cgagcgcgcg
    15121 atccatgatc tgaccactgt cggcttcggt ggtgtgcggg ccgaagttca tggtgccgag
    15181 gcagaggcgg ctgacgcgca aaccggagcg gccgaggtga acgtattgca tggggcctcc
    15241 ttggctagag caatcagaat accggcttcc gatggatact gacaaccaag ctccgcacgc
    15301 gtggatagct gcggtgcgac ggttggatgc agcaggtgag tttctgctgg cggttcggaa
    15361 cggtttggct ataatgccga tcgtatgagc acaacaaatc cagccgcgcc cgcggcaccg
    15421 cttactcctc ctgatcctga acgtattgcc cagacggtcg ccactcgatt gcactttcgc
    15481 ggagagatga ccgcactcag cgcgctggcc ggtgacgcat ccaatcgccg gtatttccgg
    15541 atctttctga agggcacgcc ctccgggctg attctcatgc agctcgccga cccggaaggg
    15601 ttcaagaaat ccgaagaagc cgtaagcggc gcgtctgctc aaattaccga gttgccgttc
    15661 atcaacgtgc tgacccactt gcaacgtacc ggcgtgaccg ttccgacctt gcactacttt
    15721 gatcgcgaag cgggtctgtt gtatctggaa gattttggag acctgaccct cgcggaagcc
    15781 tgccggggtg cagatcccgc gaggctcgag gccttgtatc gccaggcgat tgatcaactg
    15841 gttttgctgc aagtcaaagg cacgacaccg cctgcgccgg gatgtattgc gtttcaccgg
    15901 cgcttcgatg tgccgctgtt gatgtgggaa ttcgaccact ttctcgaata tggcattacg
    15961 gcgcgtctag ggcagcccat gaaaccggaa gatgattccg ccgtgcgcgc ggcgtttcag
    16021 cgcatcgccg aattattggc ggggcaaccg caggtattcg ttcatcgcga ctatcattcg
    16081 cgtaatctca tggtcgatgg atcgcgcatg ggcatcatcg attttcagga tgcccttatg
    16141 ggaccggcca cgtacgatct ggcgtcgctg ttgaaggatg cctatatcga attggatgac
    16201 gcagtcgtcg ataggctagt cgagcatttt ctcgatggcc ttgccggcct gggcgtgaag
    16261 gtcaatgatc gttcagcgtt tcgacgtgtg ttcgatctca ccagtattca gcggaatctc
    16321 aaagccgccg gccggtttgt atatatcgat cgggtgaaac acaatccgaa atttcttgcc
    16381 gatatccctc gtgttc
//
LOCUS       DAOQEW010000077        16203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_965_length_16203_cov_5.698786, whole genome shotgun sequence.
ACCESSION   DAOQEW010000077 DAOQEW010000000
VERSION     DAOQEW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_16203_cov_5.698786"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PLS35_09945"
     CDS             <1..632
                     /locus_tag="PLS35_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI19029.1"
                     /translation="HGLDLTAEAVFRAKTRFELFQLPYQAVKQGSATAIPYPDKSFDI
                     VYSHGVLHHIPPIRQVSPEIRRVLKPGGKLVIMMYHKHSLNYLVSINIVRRLLMIGLF
                     GLAKIGARPLITHPLLAGHLQNAEEFGLWTYLQNPLFMMRNTDGPGNPYSKVYDAQDI
                     AADFAEFRLENTRVHFLNERHLPVLKLLPPRVRTYLASKLGWHLWATLG"
     gene            667..2568
                     /gene="asnB"
                     /locus_tag="PLS35_09950"
     CDS             667..2568
                     /gene="asnB"
                     /locus_tag="PLS35_09950"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNI19030.1"
                     /translation="MCGIVGFMAQDAESALTERTLTIMRDQMIHRGPDDGGVYVRREG
                     ASTIGLGHRRLSIIDLSPLGHQPMSTADGSLWIVFNGEIFNYRELRAELPARIQAQFR
                     SQTDTEVILYAIREWGLDGCLQRLRGMYAFAVYDSTSEILTLVRDPLGVKPLYYTHDR
                     GRVTFASEIKALAAMPGFKPVINDQALYHYLTFANAPAPQTFFEGILKLEAGTYLTCD
                     KKGSMRQHRYWNPSTFQPGSASLTEQDYVDELRRLLRQSVRRRMVSDVPFGVFLSGGV
                     DSSLNVALMAELLDRPVETFSIGIKDDPSNEFDYARQVAEHFHTNHHEAVISDEDFIR
                     FLPQMAYLQDEPVADPVCVPIYYISKLARESGTPVIQVGEGSDEIFAGYQTYHLFDDW
                     NRRYYEPYLQAPEFVRRAVYSLGSHVCSSEKQDALRRAMLGDPLFLGNAIAFWDNEKA
                     GLLHQAFLPRHTSSEYIRNLGGSLGIDDSLLKIIHVELKNRLPELLLMRVDKMSMANS
                     IETRVPFLDEDVVSFALTIPSALKYKQNQPKYILRQAARGIIPDSIIDRKKWGFCGSA
                     TNMLSTRLTDYARSRILDSPLMHERFDRNAIEVIFIALRQQKRFNSFKIWNLLNLVLW
                     YERWFASTR"
     gene            2853..3542
                     /locus_tag="PLS35_09955"
     CDS             2853..3542
                     /locus_tag="PLS35_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19031.1"
                     /translation="MMKRALNLSLSLLVLAVGSVLPIGEETRAEAALVTYEFSGNISE
                     VPGSLFTSGGTGANGFNTGLKLSGTYTFDTAAAGLLPSSLPIQLYNNSVSSLNLSIGN
                     YSAVLQGGGTNLTTVSSGNPAFYSVNVGTAGNLVKGLAPSLFSINLADETGTAFSSVT
                     LPGNQAPNLNAFNVNRWTLAFSNGKTLVGSLDTLKAVPLPATVLLFGAGLIGLVGLGA
                     RGRFNQSSVRG"
     gene            3837..5996
                     /locus_tag="PLS35_09960"
     CDS             3837..5996
                     /locus_tag="PLS35_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNI19032.1"
                     /translation="MKQVIQNFRSGVLKIDDVPPPMLRDGGLLVMNHVSLISPGTEKS
                     TVQVAQKSLLGKAMERPEMVKKVLTAIQKDGLKDTLQRVFERLDTPAALGYSCAGTVA
                     AVGADASQFSVGDRVACAGQNYASHAEVVYVPKNLCVKLPTTVDFEDAACVTLGAIAL
                     QGVRQAEPRLGDRIAVIGLGLLGQLTVQMLKAAGCRVLGSDLDGTKLDLARQLGADLT
                     VRPSGLMDAATGFSEGHGVDAVIITASTKDNSPVEIAGAIARKKGRVVVVGAVGMNLP
                     REPYYKKELDFKLSMSYGPGRYDSAYEEKGQDYPYSYVRWTENRNMAAFLDLVGDGKV
                     QLKPLITHRFEISKADDAYRMMTEGQTPYMGMLITYPLDQQQALQPSITVVSDKPTGR
                     LTLGLIGVGNHVKDMLLPQLQSQAAVSIRAVCTASGINAKALGEKLHAGYCTSDSQDV
                     LRDPAINTVLIGTRHDTHGRLVVKALEADKHVFVEKPLCLTEEELDAIHAVYCDKAAK
                     GLRLMVGFNRRFSPHAEKAREFFAHRENPLVMWYRINAGKIPAEHWIQDPSVGGGRLI
                     GEACHFVDYMQSVCGARPNSVFARRIGRQSSGITDDQCIITLTFEDGSIGSIIYTAGG
                     NSGLAKERFEAHADGKSLTMDDFEETHLYSGSHREVFKTSKRDKGFKTEIAHFVQTVE
                     QGIPCVMSFGEIAAVTRACLLAVRSLQSGSAHDIAYV"
     gene            5989..7932
                     /locus_tag="PLS35_09965"
     CDS             5989..7932
                     /locus_tag="PLS35_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNI19033.1"
                     /translation="MFSIDFSVNTKVLPKGLAWYAKRLSVMGPGEIMHRMREGIAMQS
                     LQLQYRLSKRATAQHYGPKDIPQHVFCSGQDQRLPSLPWKFHLAQEEIACLLNGTLSA
                     LSHEWTWRADAKVWHEAPDTRQLWPQIFFHRIPYREGNPFGDIRIAWEPSRLQHLLTL
                     ALYAQQAEPDKRRQAVAMIEGQLLSWFAANPPLTGIHYISVMECALRLMAVCHTLDLV
                     RPWLSQPEAIWAGTLNLVSTHAELIRKRVSGHSSSGNHTIAEAAGLVYAGLLFPKMEM
                     AERWLAYGLYLLEQEAPHQIRQDGGGREEAIWYLRFISDLYGLTVALLRQHEQLIPQK
                     LIQATERSHTFLQAMMRPGDGALPRIGDADSGYALSPFLQFVPVSAEAAPGLTSFHLS
                     GCSILQGSKGERLLFDHGELGMAPCYAHGHADALSVQLEIGQQEILIDPGTYSYTGHS
                     EWRRYFRSTRAHNTVTVDGRDQATQETSFLWSQPFETHLIYRDETPEGKITVLARHYG
                     YTQPCGVTHWRGVVYQPPGAWLIWDWLTGTGTHHLELNWHLGVSPVQNSASYVLACRE
                     QPVQISVEGGLASLHHGETAPPSGWRSSTYGSKEPITTLRAEYRGALPHEFLTRIWCG
                     EGPPPTQLQTFSLSRSFHGSNTR"
     gene            7913..8647
                     /locus_tag="PLS35_09970"
     CDS             7913..8647
                     /locus_tag="PLS35_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNI19034.1"
                     /translation="MVPTRVDILNIPVDCVTMDQAVAWADSRMTLSEPKTVIAVNPEK
                     IMKAQSDPTLRQFLRSAGLLIPDGIGVVIATKLLGLGYAERVPGSELMPRLCGLAAEK
                     GYSIFLFGASPEVNERAAHKLRQEFPGLTIAGTQHGYVDDADMPAVIDHINAVAPHFL
                     FIALGSPKQELWMSTYLPRLRVKVCQGVGGTFDVLAGRVKRAPWIFRCIHLEWFYRLL
                     SQPSRLVRQTALPLFAFHILRKRVLG"
     gene            8644..10263
                     /gene="xrtD"
                     /locus_tag="PLS35_09975"
     CDS             8644..10263
                     /gene="xrtD"
                     /locus_tag="PLS35_09975"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM-specific exosortase XrtD"
                     /protein_id="HNI19035.1"
                     /translation="MRSTPELATKTARFLAEPVASVSAAAPTRSLALGLTVLLPVTIL
                     GLVFTDSVAYMADLWLTDENYGHGMLVPLISLYLIWQQRRRLMEQSRSGSWWGLIWIL
                     LGAALYVVGELATVYQFLHVALWCIIVGLLVSALGARAVSTIAFPLGFLLTVVPLPQF
                     LYQGLSGKLQLISSALGVGCLQFIGITAFRDGNVIDLGPIQLQVVDACSGLRYLFPLA
                     TLALLCSYLFRERLWKRIVLFISSIPISILLNGFRIGMIGVLVEHFGQGAAEGFYHAF
                     EGWVLFMASLGLLLAEMFVLRKIGATRVTRDTPSQPQPEMVPPASLPSRAILLRPTYL
                     YSVGLVCLLPFAATELTERGDVPPVRQPFLDFPMQFKGWSGTPFPLEGRYVEVLKFDD
                     YVLADYQQPQSAAVNLYIAYYRSQRKGQSAHSPQSCIPGGGWEIGTIETVQLDSAAGQ
                     APANRVSIQKGDQRQLVLYWFKQRHRIVANEYLVKLYVLWDAMTRQRTDGALIRLTTP
                     IQQNESEGAAEQRLLHFAQDMNPILTRYVPD"
     gene            10283..11911
                     /locus_tag="PLS35_09980"
     CDS             10283..11911
                     /locus_tag="PLS35_09980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNI19036.1"
                     /translation="MISTLFFSFVGSLIICMALIPPLMATASRLQFLDIPKDQRRVHA
                     IPVAKVGGIAFGVGTFSAMLIWAPKDDIVVAGLLGGVTILLFGMWDDRVGLPYTIKFL
                     GQTLGALIIMWQGNIHLNSLPFVETELPLWVAYPLTTLIIVGVTNAVNLADGLDGLAG
                     GLSLLSFAGMACLAYLGGDQTVTLLMVPVLGGLLGFLRFNTYPARIFMGDAGSQFLGF
                     FMAVSAIVFTDPQRGPYTPALGLFLLGLPLLDTVGVMLQRLKEGRSPFVADKNHLHHK
                     LLAMGLSHYEAVLMIYLLQAALVTLAYILRWQSELAILSTYGLVATMILYPFLRSRSW
                     KIQSNRMLKTADRLLTRASTITSSPWLTTEPIQILGIAVPAFLILSVFLPKHVPDDMG
                     YVAAVMFAVMLIGLWASPRISSLVVRGGLYVGSTFIMYLSEQPASSTDILSPIHIFFV
                     GLAVLVALTIRFNSEHRFQTTPLDYLMVFLAIMVPALPEMQMGDIHLSFLTAKLIVLF
                     FSFELLLHVFARRLTQLALVALWLLCGIAVRAWI"
     gene            11929..14334
                     /locus_tag="PLS35_09985"
     CDS             11929..14334
                     /locus_tag="PLS35_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI19037.1"
                     /translation="MATTWHRCHTIARFGLAIVLILAVGCGGPEERKAKYRTKAQEYL
                     QAGNFPKARVALRNVLKIDPKDSEAYFLYAQVEEKEKNWRNAFAHYQRVIELEPDHER
                     ALVKLGKFYLQGRLDEKAHETADHLLRVYPGHVSAQAIKAALLAADGKLTQAVVAGEA
                     LFKQFPTEPDAAILLSTLYALQNSPQRVEAVLRKAVAAHPHDLDLLNSLAMAYMRAGN
                     QAQAESVFRDILALEPSVFDHHLKLALFFDQQRQYDKAEAILREALRLDQDNEQRHLA
                     LADYLAGRKNVETAEAALNEAHRVLPHAVNIQFALGRLYELHQLPDKARATYEDIRDH
                     NRSKPAGGNAKIKLATLDWAAGKQDDAMQQLREVLRDNPRASDALMLEGRIALQHGSA
                     REAVQAFRTVLKDQPEQSEAYALLGQAHLMLEETSLARENLERAVTLSPNLYQAHLAL
                     AILDASSGRTRDARVRLEELLKQAPTNLGTLGMLLNLQAADRDWDGSDQTLSRIREAG
                     ANRFVADLAEGNLYQARLQWDKAVAAFERASSLNPDAPEPIFALVRIDARLGKLAQAH
                     NRLSGLIAKNAQHPFAHGLLGEILILKGDPMRAEQEFRLATTLKPEWGTPWVNWVTLK
                     LSQRKHEEAGDILQRGLQINPASEELRLLYASHLGERGQFDQAILEYETILKHNPRQL
                     MAANNLASILADQKGDAKSLDRALALSREFEQRAPNPFFLDTLGWVHLKMGHQDDAIR
                     VIQRAVTEAPQHPILNYHLGMAHFKAGHRVEAQTHLKKALNTKQPFPGMDEAKSVLAE
                     VTG"
     gene            14346..14963
                     /locus_tag="PLS35_09990"
     CDS             14346..14963
                     /locus_tag="PLS35_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNI19038.1"
                     /translation="MKIAGSLLIKKLARGCQPGLLALYAVVLMTGCVTPEHTAAQNMA
                     DPGYHLGPEDVVMISVWKDENLTREAIVRPDGMVSFPLIGDVPAMGHTVEEVRQDIAK
                     RLARFIPNPHVSVAITKLQSYKVYVMGRVTKPGEYLVGHYTDVLQALSLAGGLTPFAS
                     ENDIKIIRRENGDQRVFLFRYGDVQKGRDLNQNILLERGDVVMVP"
     gene            15004..>16203
                     /locus_tag="PLS35_09995"
     CDS             15004..>16203
                     /locus_tag="PLS35_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19039.1"
                     /translation="MKHRLIPTAWLWAILLTTAWSSHSLDQEVWSAEWFLEPALTVRG
                     EVNSNLTLTTFDHKTTYGHWVSPGAKFGGSTEALEVNGRLAADFVRYYGGQNTSLTNL
                     YFPLSAKYKMERDSFGFDGGFTRDNTLMGELRQTGVVLSFTQRNQWILNPSWTHAITE
                     RLSATGSYTFYDTRYENGLRLGLADYTLHVGSTGMTYQVGERDQVQLNAVGVRFNAAD
                     LRLSSTIIGPQITYSHMFSETLTGTVSGGPRHVSSTINTSAGSLSDGVWTWVFSGNLE
                     RRWEDWRVKGEIGREINPSGFGLLLRTDKLSLEVSHQMTDRLSASVTGLFLIADGVKT
                     RAVSTVFPENRLFYVTPKLSWRLSEWWTADITYTYAQRDLPSQSLTALGNSATIMFTY
                     YPPRFTVG"
BASE COUNT         3503 a         4588 c         4369 g         3743 t
ORIGIN      
        1 ggcatggtct tgacctgacg gcagaggccg tgttccgcgc caaaacgcgc tttgaactct
       61 tccaactccc gtaccaggcg gtcaaacagg ggagtgccac ggcgatccct tatcccgata
      121 aaagctttga catcgtctac tcccatggtg tgcttcatca catcccgcca atccgtcaag
      181 tcagtccgga aattcgccgg gtgctgaagc ccggtggcaa attagtcatc atgatgtatc
      241 acaaacattc gttgaactat ctggtgagca tcaacatcgt tcgccgcttg ctgatgatcg
      301 gcctctttgg tctggcgaaa attggcgcac gtccgttgat cacacatcct ctcctggcag
      361 gccatctaca aaacgccgag gaattcggcc tctggaccta cctccagaat ccactcttta
      421 tgatgcgaaa tacagatggg ccgggcaacc cctattccaa agtgtatgac gctcaggaca
      481 ttgcggcgga cttcgccgag ttccgcctcg aaaatacccg tgtacatttt ctcaatgaac
      541 gccatctgcc ggtgttgaaa ctgttgccgc cacgtgttcg aacatatctt gcgtcgaagc
      601 tcgggtggca cctgtgggcc acgctgggct aatcgcgcgc atctcgccgt aggagtattt
      661 cacgacatgt gtggcattgt cggattcatg gcacaggatg ccgaatcggc cctgaccgag
      721 cgcaccctga ccatcatgcg cgatcagatg attcatcgtg gacctgacga tggaggagtc
      781 tacgttcgtc gcgaaggcgc ttccacgatc gggctgggac atcgtcggct gtccatcatt
      841 gacctctcgc cactcggtca tcaaccgatg agcaccgccg atggttcgct ctggatcgtg
      901 ttcaacgggg agatcttcaa ctaccgtgaa ctgcgggccg agctgccggc acgcattcag
      961 gcgcagtttc gctcacaaac ggataccgag gtgattctct atgccattcg agaatggggc
     1021 cttgatggct gtttgcagcg gctgcgcggc atgtatgcct tcgcagtcta tgacagtaca
     1081 tccgagatcc ttaccctggt ccgcgacccg ttaggcgtca aaccgctgta ctacacacac
     1141 gaccgtggac gtgtgacatt tgcttcggag atcaaagcgc tcgcggccat gcccggtttc
     1201 aaaccggtga tcaatgatca ggcgttgtat cattacctca cctttgccaa tgcccccgcg
     1261 ccgcagacgt tctttgaagg gatcttaaaa cttgaagccg gtacgtacct gacttgtgac
     1321 aagaagggat caatgcgtca gcaccggtac tggaatccat ccacattcca gccgggatct
     1381 gcgtcactca cggaacagga ctacgtggac gaactgcgcc gccttctcag gcaatcggtt
     1441 cgtcgccgca tggtgtcaga tgtgccgttc ggagtctttc tcagcggagg cgtggattcc
     1501 tctctgaacg tcgctctcat ggcagaactc ctcgaccggc cggttgaaac cttttccatc
     1561 gggatcaaag acgatccgtc caacgaattc gactatgcga ggcaggtcgc ggaacatttc
     1621 catacgaatc accatgaggc cgtcatctcc gacgaggact tcattcgttt tctgccgcag
     1681 atggcctatt tgcaagacga accggtcgca gaccctgtct gcgtgcccat ttattacatc
     1741 tccaaattgg cgcgagaatc cgggacccca gtgattcaag tgggggaggg cagcgacgaa
     1801 atttttgccg gctaccagac ctatcatctc ttcgatgact ggaatcgccg gtattatgaa
     1861 ccgtatcttc aggcgccgga attcgtgcgg cgtgcagtct acagccttgg gtcccacgtc
     1921 tgttcttctg agaaacagga cgctctgcga cgagcgatgt tgggtgatcc gctgtttctc
     1981 ggcaatgcca ttgcattctg ggacaacgaa aaggccggat tgcttcacca ggcgtttctt
     2041 ccgcggcaca catccagcga gtatattcgc aacctgggtg ggtcgttggg cattgacgat
     2101 tcgctcctca agatcattca cgtggaactc aagaaccgac ttcctgaatt attgttgatg
     2161 cgagtcgaca agatgagcat ggccaactca attgagacca gagtgccatt cctggacgag
     2221 gatgtggttt cgtttgcgct caccattccc tctgccctga aatataaaca gaatcaaccg
     2281 aagtacatcc ttcggcaagc ggcccgtgga atcattcctg acagcatcat cgatcgaaag
     2341 aagtgggggt tttgcgggtc ggccacgaat atgctgtcca cccggcttac cgactatgct
     2401 cgttcgcgca tcctcgatag tccgttgatg cacgaacgat tcgatcgcaa cgccatcgaa
     2461 gtcattttca tagctctccg ccaacagaag cgatttaaca gtttcaaaat ctggaatctg
     2521 ctgaatctcg tcctgtggta cgagcgttgg tttgcttcca cccgataagc ctgatcgcca
     2581 ccctccggtg gcgcgccgct ccatattttt tttcttcctt cgccaactcc ccgaagtctc
     2641 tccgtagcac tcctgttttt gttccgtcgc ccaaaattga acgccctgtg tacgattcca
     2701 tacctcgctc accggctccc gccaatccat catccatatt tcaacgactt aaccacggtc
     2761 tgagtggccc aggacttgct caatccccct cgccaaccgg gaacgacact gccgtacgat
     2821 cctgaagcgt ttactcaacc gaaggagtca tcatgatgaa acgtgcactc aacctctcgc
     2881 tgagtctcct cgttcttgcc gtgggatcgg ttctgccgat cggggaagag accagggctg
     2941 aggctgcact cgtgacctac gaattcagcg ggaacatcag cgaagttccc ggctctctct
     3001 tcacgtcagg tggaacggga gcaaacgggt tcaacacggg cctcaagctg tcagggacct
     3061 acacattcga tacggccgcg gcgggattgc tgccgtcgag tttgccgatt cagttgtata
     3121 acaattccgt ttcgagcctg aatctttcga ttggaaacta ctcggcggtg cttcagggcg
     3181 gcggaaccaa tttgacgacc gtgagcagcg gcaatcctgc gttttattcc gtgaatgttg
     3241 gaacggcggg caatctcgta aaaggactgg caccgtcgtt attctccatc aatcttgcgg
     3301 atgagaccgg cacggccttc agcagtgtga ccttgcccgg caatcaagct ccgaacctca
     3361 acgccttcaa tgtcaatcgt tggacactgg cgtttagtaa cggcaagacc ctggtcggtt
     3421 cattggatac gctgaaagca gttccccttc ctgccacggt gctgttgttc ggagccggcc
     3481 ttatcggtct ggttgggttg ggcgcgcgcg gccgtttcaa ccaatcctct gtgcgcgggt
     3541 aacggcttgc cgctgacgta ccaccgcatc atgtgacttg tgttcgcaca agaccggttg
     3601 tgagaaaagt ggggcgcgca gaacattctg ccgcccgact tttctcccaa ggctccctgt
     3661 agcactttgc tctacaaaaa cacctcgtac agcgcgaatc ctgcaaaaat tccgttcaag
     3721 cattgacctt ccggacgaac gaaggtagtc tccgaaatgg gctgctcgtc ctgcctgttc
     3781 ttttgttttg attcagtatc aatccaacac ttttacgtat ttggtatcgc gacactatga
     3841 aacaagtgat tcaaaatttt cgctccggag tgctgaagat cgacgatgtt cctcctccca
     3901 tgcttcgcga tggtggcttg ttggtgatga accacgtgtc actgatcagt cctggcacgg
     3961 aaaaatcaac cgtccaagtg gcacagaaaa gccttctcgg caaagccatg gaacgtccgg
     4021 agatggtgaa aaaggtcctt acggccatcc aaaaagacgg cctgaaagat accttacaac
     4081 gcgtgttcga gcgcctcgac accccggctg cactggggta tagctgcgcc ggaaccgttg
     4141 cggcggtcgg tgctgatgcg tctcagtttt cggtcggaga ccgtgtcgcc tgtgccggtc
     4201 aaaactatgc ctcccatgct gaggtggtgt atgtacccaa gaacctctgt gtgaaactac
     4261 ctacgacggt ggatttcgaa gatgccgctt gcgtgacgtt gggagccatc gcccttcaag
     4321 gggttcgaca ggcagagcct cgtctggggg atcgtatcgc tgtgatcggc ctcggactcc
     4381 tgggccaact gaccgtacaa atgttgaaag cggccggatg tcgggtcctc ggctctgacc
     4441 ttgatggcac caagctggat ctcgcgcgcc aactaggcgc cgacctcaca gtacgtccct
     4501 cagggctgat ggatgccgcg actggcttct cagaaggcca tggggttgat gcggtgatca
     4561 tcacggccag cacaaaggac aatagcccgg tcgagattgc cggagcgatt gcccgtaaga
     4621 agggaagggt ggtggtcgta ggggcggtcg gcatgaatct gccccgagag ccgtattaca
     4681 aaaaagagct cgacttcaaa ctctccatgt cctatggacc aggccgctat gacagcgcat
     4741 acgaagagaa gggacaggat tacccctaca gctatgttcg ctggacagaa aaccggaata
     4801 tggctgcctt tctcgatctg gtcggcgacg gaaaggtgca gctcaaaccg ctcattacgc
     4861 accgctttga aatcagcaag gccgatgatg cctaccgcat gatgacggaa ggtcagacgc
     4921 cctacatggg catgctgatt acgtacccgc tggatcaaca acaggccctg caaccttcca
     4981 tcacagtcgt gagcgacaag ccgaccggtc gattgactct cggtttgatt ggagtcggaa
     5041 atcacgtcaa agacatgtta ttgcctcaat tgcagagcca ggcggccgtc tctatccgag
     5101 ccgtgtgcac ggcttccggc atcaacgcga aagctctcgg ggaaaaattg cacgcaggct
     5161 attgcaccag cgactcccag gatgtgcttc gagatccggc cattaatacc gttctcattg
     5221 gtacccgaca cgacacccac ggtcgtctgg ttgtgaaagc cctggaagcc gacaagcacg
     5281 tgtttgtgga aaaacccctg tgcctaaccg aggaggagct tgacgccatc catgccgtct
     5341 attgcgacaa ggctgcgaag ggacttcgtc tgatggtcgg gttcaatcgc cgtttttccc
     5401 ctcacgcaga gaaggcgcgc gagttttttg cccatcgcga aaatccgctg gtcatgtggt
     5461 accgcatcaa tgccgggaaa attcccgccg aacattggat tcaagatccg tccgtcggcg
     5521 gaggacgtct catcggcgag gcctgtcatt ttgtcgatta catgcagtcg gtatgcggcg
     5581 cccgtcccaa ctccgtcttt gcacgacgga tcggccgcca gagctcgggc atcactgatg
     5641 atcaatgcat catcacgctc acgttcgaag acggctcaat cggatccatc atctacaccg
     5701 caggcgggaa ttccggcctg gccaaagaac gcttcgaggc acatgcggac ggtaaatcgt
     5761 tgaccatgga tgactttgag gaaacgcacc tctattcagg ttctcatcgc gaggtcttca
     5821 aaacatccaa acgagacaaa gggttcaaaa ctgagatcgc tcactttgtt caaaccgtgg
     5881 agcaggggat tccttgcgtg atgtcgtttg gagagatcgc cgccgtaacc cgagcctgcc
     5941 tgcttgctgt caggagtctg caatccggct ctgcacacga tatcgcatat gtttagtatc
     6001 gatttctccg tcaatacgaa ggtgcttccg aaaggcttgg cctggtatgc caaacgtctc
     6061 agcgtcatgg gacccggtga aatcatgcat cgcatgcgcg aaggcatcgc gatgcaatca
     6121 ctccaattgc agtaccggct ctccaagcgc gccaccgctc aacactatgg gccgaaggat
     6181 ataccacagc atgtattttg ctcggggcaa gaccaacgac ttccctctct gccttggaaa
     6241 ttccatctcg ctcaggaaga aattgcctgc cttttgaatg gcacactgtc ggccttgagc
     6301 catgagtgga catggcgggc cgatgcgaag gtctggcatg aagcgccgga taccaggcag
     6361 ctatggccac agattttctt tcatcgcatt ccttatcgag agggcaatcc gttcggggat
     6421 attcgtattg cctgggaacc ctcccgtctt caacatctcc tcacgttggc cttgtatgcc
     6481 cagcaggcag agccggacaa acgtcgacag gctgtcgcga tgatcgaggg acaactcctg
     6541 tcctggttcg ccgcgaaccc accattgaca gggattcatt acatctcagt catggaatgc
     6601 gcacttcgcc tcatggcggt gtgccacacg ctagacctgg tcaggccatg gctgtcacag
     6661 ccagaagcga tctgggccgg aaccttgaac ctcgtctcca cccacgcgga gctcatccgc
     6721 aaacgcgtgt ccggacattc ctcctccggc aatcatacga ttgccgaagc ggccggcctc
     6781 gtatacgcag ggcttctctt ccccaaaatg gagatggctg aacgctggtt agcctatgga
     6841 ctctatctct tagagcaaga ggctcctcac caaattagac aggatggagg agggcgtgaa
     6901 gaggcgatct ggtacctccg gtttatctcc gatttgtacg gactcaccgt cgccctgtta
     6961 cgccagcatg aacaactcat tcctcaaaaa ctgatccagg ccactgaacg aagccatacg
     7021 tttcttcagg cgatgatgcg tccgggcgat ggcgccctgc cacgtatcgg ggacgcggac
     7081 agtgggtatg cgctctctcc attcctccag ttcgtgccgg tttctgccga ggcagccccc
     7141 ggcttgacct cgtttcatct gtccggctgt tcaattctgc agggcagcaa aggcgagcga
     7201 ctgctgttcg accacgggga attaggcatg gcaccctgtt atgcgcatgg ccatgccgat
     7261 gccttgtcgg tgcaattgga gatcggacaa caggaaattc tgatcgatcc cggcacctac
     7321 agttacaccg gccattccga gtggcggcgt tattttcgaa gcactcgcgc ccataatacg
     7381 gtcaccgtcg atggccggga tcaagccacg caggaaacgt cttttctctg gtcacagcct
     7441 ttcgaaaccc atttaatcta tcgtgacgaa acacctgaag gaaaaattac ggtgctggcg
     7501 cgacattatg gctacacgca gccatgcggg gtcacccact ggcgaggagt ggtctatcag
     7561 ccacctggcg catggctcat ttgggattgg ctaaccggaa ccggcactca ccacttggaa
     7621 ttgaattggc atctgggagt gtctcccgtt caaaactccg cgtcctacgt cctggcatgc
     7681 cgggagcaac cggttcaaat ctccgtcgaa ggaggactcg cctcgctcca tcacggcgaa
     7741 accgctccgc cttcgggatg gcgctcttcg acttatggat caaaggagcc cattaccaca
     7801 ctacgagctg aatatcgcgg tgcgcttccg catgagttcc tgacaagaat ttggtgcggg
     7861 gaggggccac ccccgacgca gctgcaaact ttttcgttgt ccagaagctt ccatggttcc
     7921 aacacgcgtt gacattttaa atattccagt cgattgcgtc acgatggacc aggctgtggc
     7981 gtgggccgac tctcgcatga cattgtcgga acccaagacg gtgattgcgg tcaatcctga
     8041 gaagatcatg aaagcccaga gtgatcccac gcttcggcaa tttttacgct ctgcgggact
     8101 cttgattccc gatggaatcg gggtcgtcat cgccaccaaa ctcctcgggt taggttatgc
     8161 cgaacgagtg ccagggtccg aactgatgcc acgcctctgc ggtttggctg cggagaaggg
     8221 atatagcatc tttctgttcg gtgccagtcc tgaagtgaac gaacgggctg cacacaaact
     8281 tcggcaggaa ttcccgggac tcacgattgc tggaacgcag cacggatatg ttgacgacgc
     8341 agacatgccg gcggtcatcg atcatattaa tgccgtcgct ccgcatttcc tctttatcgc
     8401 gctcggcagt cccaagcagg aactgtggat gtccacgtat cttcctcgct tgcgcgtgaa
     8461 ggtctgccaa ggcgttgggg gaaccttcga tgtcctcgcc ggccgggtca aacgcgctcc
     8521 atggattttt cggtgcattc atcttgaatg gttctatcga ttgctgtcac aacccagccg
     8581 ccttgtacgg cagaccgctt tacctctttt cgcgtttcac attctgcgaa aacgagtatt
     8641 aggatgagat ccactcccga gcttgcgacg aaaacggctc gcttcttggc agagccggtt
     8701 gcctccgtct ccgcagcagc accgacccgc tcacttgcgt tgggcctcac ggtgcttctg
     8761 cccgtgacca tactcggcct agtcttcaca gacagcgtgg cctacatggc tgatttgtgg
     8821 ctcaccgacg aaaattacgg gcacggaatg ctggtgcctc tgatcagcct gtaccttatc
     8881 tggcaacaac gacgacgcct catggaacag agccggtccg gatcgtggtg gggtctcatc
     8941 tggattctgc tcggagccgc cctgtatgtc gtgggtgaac tcgccaccgt ctatcagttt
     9001 ctgcacgtcg cattgtggtg catcattgtg gggttgttag tgtctgcatt aggggctcgg
     9061 gccgtctcca cgattgcctt tcctttggga tttcttctca ccgtcgtccc cttgccacag
     9121 ttcctctatc aagggctctc ggggaaattg cagctcatct catccgcgct cggcgtcggg
     9181 tgcctccaat ttatcggcat cacggccttt cgggacggga atgtgattga tttggggccc
     9241 attcagctcc aggtagtcga tgcctgcagc ggcctgcgat accttttccc gctggcgacc
     9301 ctggcactgc tctgcagtta tctcttccgg gaacgcttgt ggaaacgcat cgtgctgttc
     9361 atatcgagca ttcccatttc cattcttctc aacggattcc gcatcgggat gatcggtgtg
     9421 ctggtcgaac atttcggcca gggtgcggca gaagggttct atcacgcatt tgaaggctgg
     9481 gtcttattca tggcgagtct gggcttgctc ctcgccgaaa tgtttgtcct acgcaaaatc
     9541 ggcgcgacgc gtgtcacacg agacacacct tctcaacctc aacctgaaat ggtgcctcct
     9601 gcatcactgc cttctagagc catactgctc cgcccaacgt atctgtatag cgtcggcctg
     9661 gtatgcctgc tgccgttcgc cgccacagaa ttgactgagc ggggagacgt gccgcccgtt
     9721 cggcagccgt tcctggattt tccgatgcag ttcaaaggat ggtccggtac cccgtttccc
     9781 ctcgaagggc gctatgtcga ggtgctgaag tttgacgact acgtattggc cgactatcag
     9841 caacctcaat cagcggcggt caatttgtac attgcttact atcggtctca acgcaaagga
     9901 cagtccgctc attcaccgca gagttgcatt cccgggggag ggtgggaaat cggcacgatt
     9961 gagacggtgc agcttgactc ggccgcaggg caggcacccg ccaatcgtgt ttcgatccag
    10021 aaaggtgatc agcgccaact ggtgctgtat tggtttaaac aacgccatcg catcgtggcg
    10081 aacgaatacc tggtgaaact gtacgtcctg tgggatgcca tgacacgaca gcggaccgac
    10141 ggcgcattga tacgattgac caccccgatc caacagaatg aaagcgaggg cgcggcagaa
    10201 cagcggctcc tgcacttcgc tcaagacatg aatccgatct tgacccggta tgttcccgac
    10261 tagactgagc agatagcaca gcatgatttc cacattattt ttcagctttg tcggttcgct
    10321 gatcatctgc atggcgctca tcccgccatt gatggcgact gccagccgcc tccaatttct
    10381 ggatattccg aaagatcagc gccgggtgca tgccatcccc gtggccaagg ttgggggaat
    10441 cgccttcggg gtcggcacgt tttccgcaat gctgatctgg gcgccaaagg acgacattgt
    10501 ggtggccgga cttctcggcg gagtcaccat tctgctgttt ggtatgtggg atgaccgggt
    10561 tggcttgccg tatacgatca aatttcttgg gcaaactctc ggcgcgctca tcatcatgtg
    10621 gcagggcaat attcacctca actcgctgcc atttgtcgaa acggaactcc ccctgtgggt
    10681 cgcctatccc ttgacgacgc tgatcattgt cggggtgacg aacgccgtga atcttgccga
    10741 tggattggac gggctcgccg gcggcttgtc gttgttgagc ttcgcaggca tggcctgcct
    10801 agcgtacctg ggaggagatc aaaccgtcac actgctcatg gttccggtcc tgggaggatt
    10861 gctgggattt ttacgcttca atacctaccc ggcgcggatc ttcatgggag atgccggaag
    10921 ccagtttttg ggatttttta tggcggtctc tgcgatcgtc tttaccgatc cgcaacgcgg
    10981 tccctatacg ccagccctgg gcctgttcct tcttggcctt ccgctactcg acaccgtcgg
    11041 agtcatgttg caacggctga aggaaggacg gtcgccgttt gtcgcggaca aaaatcattt
    11101 gcaccacaaa ttgctcgcca tggggctctc ccactatgag gccgtcctga tgatttatct
    11161 tctgcaggcg gctctcgtca cgctggccta tattctgcgc tggcagagtg aattggccat
    11221 cctaagcacc tatggcctcg tggcgaccat gattttgtat ccgttcttac gctcccgctc
    11281 ctggaagatt caatcgaatc ggatgctgaa gacggccgac cgtctcctga ctcgagccag
    11341 caccattacc tcttcgccct ggctgacgac tgaaccgatc cagattctcg gaattgccgt
    11401 gccggcgttt ttaattctca gcgtcttttt gcccaaacac gttccggacg atatgggcta
    11461 tgtcgccgcg gtgatgtttg ccgtgatgct gatcggactg tgggcctctc ccaggatttc
    11521 atcgctggtc gtccggggcg gactctatgt cggaagtacg ttcattatgt atctcagtga
    11581 gcagcccgct tcttccacgg acattctcag ccccatccat atcttctttg tgggcctcgc
    11641 cgtgctggtg gctctcacca tccgtttcaa ctctgaacat cggtttcaaa cgacgccctt
    11701 agactatcta atggtgtttc tcgcgatcat ggtccccgca ttgccggaaa tgcagatggg
    11761 cgacatacat ttgagcttcc tgacggcaaa attgatcgta ctattctttt cttttgaact
    11821 cctcctccac gtattcgccc gccggctgac tcaactggca ttggtcgctc tctggctgct
    11881 gtgcggcatc gccgtgcgag cctggatata acaaggaagc aagacactat ggctacgaca
    11941 tggcatcgat gtcacacaat cgcgagattc ggcttggcca tagtgctgat tcttgcggtt
    12001 ggctgtggag ggcccgaaga acgcaaagcg aagtaccgca ccaaagcgca agaatatctc
    12061 caggcaggga attttccgaa agcccgcgtt gcacttcgga atgtcttaaa aatcgatcca
    12121 aaagattcgg aagcctattt tctctatgcg caggtcgagg agaaggagaa gaactggcga
    12181 aacgcgtttg cgcactacca gcgggtgatt gagcttgagc cggatcacga gcgtgcactc
    12241 gtcaaattag gcaaatttta tttgcaaggc cgcttggatg agaaggcaca tgaaacggcc
    12301 gatcacctgc ttcgggtata cccaggacac gtctccgcgc aagccatcaa agcagcgctt
    12361 cttgccgctg acggcaaact tacccaagcg gtggttgccg gcgaggctct ctttaagcaa
    12421 tttccaaccg aaccggatgc agccatcctg ttgagcacat tgtatgcgct tcagaactcg
    12481 cctcagcggg tcgaagccgt tctccgaaaa gctgtggcag cccatccgca tgaccttgat
    12541 ctgctgaatt cgttagccat ggcgtatatg cgtgcaggca atcaggcaca agcggaatcg
    12601 gtgttccgtg acattctggc tctcgaaccc agcgtcttcg atcatcacct caaactcgca
    12661 ctctttttcg accagcaacg gcaatatgac aaggccgaag cgatcttgcg cgaagcgctg
    12721 cgtctcgatc aggacaacga acagcgacat ctcgcgctcg ccgattatct ggcaggtcga
    12781 aaaaatgtgg agacagcgga agccgctctc aacgaagccc atcgggtact tcctcatgcg
    12841 gtgaatattc aatttgcact gggacgtttg tatgaattgc atcaactgcc tgacaaggca
    12901 cgcgcgacct acgaggacat ccgcgatcac aatcggtcta aaccggcggg agggaatgca
    12961 aagataaagc tggccacgct cgattgggct gcgggaaaac aggacgacgc catgcaacag
    13021 ctgcgtgaag tgcttcgcga taatccgcgt gcgtcggatg cgctcatgct agaagggcgg
    13081 attgcgcttc aacatggttc cgctcgagag gcggttcaag cctttcgcac cgtgctcaaa
    13141 gaccagccgg aacaatcaga agcttacgcg ttgctaggac aggctcattt aatgttggag
    13201 gaaacaagct tagcccgtga gaaccttgaa agagccgtaa cactcagtcc gaatctctat
    13261 caggcgcacc tggcactggc cattcttgat gcctcgtccg gacggacaag ggacgcccga
    13321 gtgcgcctgg aagaattgct caaacaggcg ccgacgaatc tcggaacatt gggcatgctg
    13381 ttgaatctgc aggcagccga ccgtgattgg gatggatccg accagacctt atcacgcatt
    13441 cgcgaggccg gtgccaaccg gtttgtagcg gatctcgccg agggcaatct atatcaggcc
    13501 cgcttgcaat gggataaagc tgtggcggcc tttgaacggg cttcttccct gaatcctgac
    13561 gcgccggaac cgatctttgc attggtccgc atcgatgcac ggttaggcaa gttggctcaa
    13621 gctcacaatc gcctcagtgg cctcatcgca aaaaacgcac agcatccatt cgcgcatggc
    13681 ctactgggag aaattcttat cctcaaaggc gatccgatgc gggcggaaca agaatttcgg
    13741 cttgccacta ccctcaagcc ggagtggggc actccttggg tcaactgggt cacgctcaaa
    13801 ctctctcaga ggaaacacga ggaagcaggc gacattcttc aacgcggatt gcagatcaac
    13861 cccgccagcg aggaactccg cctcctctat gcctcgcacc tgggtgagcg tgggcagttc
    13921 gatcaggcca tcctggagta tgaaacgatt ctgaaacaca accccaggca actgatggcg
    13981 gccaacaacc tcgcttcgat tctggccgat cagaaaggtg atgcgaagag cctggatcgg
    14041 gcgctggcgt tgagccgcga gtttgagcag cgcgcaccaa atccattttt tctcgacaca
    14101 ctggggtggg tccacctaaa aatgggacat caggatgatg ccatccgtgt gattcaacgt
    14161 gcggttacag aggccccgca acatcccatt ctcaattatc acttgggcat ggctcacttc
    14221 aaagccggcc accgggtgga agcccaaacc catctcaaaa aagcactcaa cacgaaacaa
    14281 ccgtttcccg gcatggacga agccaaatcg gtccttgcgg aagttacagg ataggaggaa
    14341 agagggtgaa gattgccggt tcgttactga tcaagaaact agcccgaggt tgccagccag
    14401 gtttgcttgc gctctacgct gttgtgctga tgactggctg tgtcacgccc gaacacactg
    14461 ccgctcaaaa catggccgat ccgggatacc atctcgggcc ggaagatgtc gtcatgatct
    14521 ccgtgtggaa agacgaaaat ctcacacgag aggcgatcgt tcgtccagat ggcatggtgt
    14581 cgtttccgct catcggggat gtgccggcta tgggacatac cgttgaagag gtgcgtcagg
    14641 acattgccaa acgcttagcc cggtttattc ctaatccgca tgtctccgtg gcaatcacaa
    14701 aactccagag ctacaaagtg tatgtcatgg gtcgggtcac gaagccgggc gaatacctcg
    14761 tgggacacta taccgacgtt ctacaggccc tgagccttgc cggcggtctt acgccgtttg
    14821 catcggagaa tgacattaag attatccgcc gggaaaacgg tgaccaacgc gtcttcctgt
    14881 tccgatacgg cgacgttcaa aaaggcagag acctcaatca aaacatcctc cttgagcggg
    14941 gggatgtggt gatggtgccg tgatgcatca tcgaaactcg atcggcatcg cgaacgtgtc
    15001 gcggtgaagc atcgtcttat tccaacagcc tggttatggg ccattctttt gacgaccgcg
    15061 tggtcgagtc atagcctcga tcaggaggtc tggtctgcag aatggttttt ggaaccggcc
    15121 ttgacggtgc gcggggaggt caatagtaac ctgactctca ccacgttcga ccataagacg
    15181 acgtatggtc attgggtatc ccccggtgcc aagttcgggg gctcaacgga agcgctggag
    15241 gtcaacggac gattggcggc ggattttgtt cgatactatg gagggcagaa taccagcctt
    15301 acgaatctgt atttcccgct ttccgccaag tacaaaatgg agcgtgacag cttcggtttt
    15361 gatggcggat tcacacgcga taataccctc atgggcgaac tgcgacagac aggcgtggtg
    15421 ctcagtttca ctcagcggaa tcagtggata ctcaacccgt catggacgca tgccattacc
    15481 gaacggctct ccgcaacggg ctcttacacg ttttacgaca cgcgctatga gaacggcctg
    15541 cgcctcggat tggcagacta taccttgcat gttgggtcga cgggcatgac ataccaggtt
    15601 ggtgagcgcg atcaggtaca gctgaatgct gtgggcgtgc gtttcaatgc cgcagatctt
    15661 cggcttagtt caacgattat cggccctcag atcacctatt cacacatgtt ttctgaaacc
    15721 ttgacgggaa ctgtatcggg aggaccaagg catgtgtcgt ccaccatcaa cacttccgcc
    15781 gggtctttat ccgatggtgt gtggacctgg gtcttttcag ggaacttgga aagacgatgg
    15841 gaagactggc gggtgaaagg tgagatcgga agagaaatta acccgagcgg attcggcctg
    15901 ctgctccgta ccgataaact aagcctggaa gtgtcccatc aaatgacgga tcgcctgtcg
    15961 gcttcggtca ccggcttgtt tctcatcgcc gatggggtga aaaccagggc cgtttccacc
    16021 gtgttccctg agaaccggct cttttatgtg acaccgaagc tcagctggcg gctgtcggaa
    16081 tggtggacgg cggacattac ctatacgtat gcccaacgag accttcccag ccaatcgttg
    16141 accgcactgg gcaattctgc aactattatg tttacgtatt accctccaag gtttacggta
    16201 gga
//
LOCUS       DAOQEW010000078        16063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_979_length_16063_cov_8.158421, whole genome shotgun sequence.
ACCESSION   DAOQEW010000078 DAOQEW010000000
VERSION     DAOQEW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_16063_cov_8.158421"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="PLS35_10000"
     CDS             <1..1660
                     /locus_tag="PLS35_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNI19040.1"
                     /translation="MTLVVLADNGFFFLIVWEVMSLLSYVLVVTEHERPGVREAGLFY
                     LIMTHAGTAFIILTFLLFSQETGSFSFEAFRHPDQRLPDGLRSIAFFTALIGFGAKAG
                     IVPLHVWLPYAHPAAPSHISALMSGVMIKTAIYGLIRVYFDFLEGQFPWWWGFTVLSI
                     GAVSALLGVMYALMEHDLKSLLAFSSVENVGIILLGIGAGMIFHTYGLHELAALGLLA
                     GLYHTINHAAFKGLLFLGAGSLLFVTHTRNIEEYGGLLRRMPWTGLCFLIGAVSIVAL
                     PPANGFVSEWLVFQSLFLSVQLPTLFLKLMLPIAAAILALTGALALACFAKAFGLSFL
                     AQPRSAHARHAVEVPVSMRIGMGLLASSCALFGLAPMLVVPLLDRVTTPLAGVAISEK
                     VLALDGWVVAPVTVEFSSISMPVLAALLVGAAGLGLAIARLLRKGVHARYSRTWGCGL
                     PLTPRMEYSATGFVQPIKQVFEMIYQPTVKLEREFLKQSKYFIRHQRFHSSIEPLFEK
                     YAYRPIVNGLLAAAERLRVIQAGSLHLYLAYIFVTLVLLLLWAG"
     gene            1723..2679
                     /locus_tag="PLS35_10005"
     CDS             1723..2679
                     /locus_tag="PLS35_10005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNI19041.1"
                     /translation="MQVISQIGFVFIQLIILLAFSPFIVGVIRKVNARLQCRRGASLL
                     QPYADLAKLFGKQPVISSTTSWIFTAAPYIVFASTVAAGLLMPVFISHTPLNFAGNII
                     ALVYLLALGTFFLILAGLDAGSAFGGMGSSREAIVVTLAEPAMILSILAIALTAGSTN
                     LSTIVHQSALLEGMVLAPPAHLMALAAMFIVTLAETGRVPVDNPATHLELTMIHEAML
                     LEYSGRYLALMEWAAGIKLLVFLTLLVNVFAPWGIATEATLSALATGVAVYVAKVTGL
                     AVIIGVIESMFAKLRLFRVPELLGSAFILALLALVFFYTLRG"
     gene            2684..3349
                     /locus_tag="PLS35_10010"
     CDS             2684..3349
                     /locus_tag="PLS35_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNI19042.1"
                     /translation="MSESTQLGSQLVDLCSVLLLLSCFAIVAQRRLSACVDLFALQSF
                     FLATTAALVAFLTGHRHIYLAAALTVVIKVIVLPRILRKVIERLNVSRELVMNINIPA
                     GLLICGGLVVVAFFITQPIIPLGYLLTRDSLALALAIILIGLFTMIARKKAVTQMVGF
                     LVMENGVFLGTTAAAYGMPLIVELGVFFDVLVAGLIAGIYTHRLQDAFDSVDTSELTV
                     LKE"
     gene            3386..4918
                     /locus_tag="PLS35_10015"
     CDS             3386..4918
                     /locus_tag="PLS35_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNI19043.1"
                     /translation="MWPVIVLLAGPVLAGLLSLVIQRARLLHAVNFTTMLGLAVAETF
                     LTKRVLAEGSVTTLDSLVYIDALSDFILIIITTIGLSCSLYMWSYMDEQVARGVIAPK
                     HLGVFFFLFHMFLFAMVAATMANSLGVQWVAVEGTTLATTFLIAFFRRRESLEAGWKY
                     LILCSVGIALALFGVVLTYYSSVRVLGDASSALNVTALIGVADRLDPNVLKLAFLFIL
                     VGYGTKVGLVPMHTWLPDAYTEAPAPVAAMLAGVLETVAVYTVLRSKALVDQALPPQF
                     TGNLLLTFGFVSFVVASLFILLQHNYKRLFAYSSIEHMGLAMLGFGVGGLVGTFGGLF
                     HLLNHALAKALAFFVAGNIHRRYDSLEIDHVRGLARSQPITALATLVAGFALVGLPPF
                     SPFVSELLVVSALSAQNFASDTMHVGRFVAVTVSDEMRSLGLVGVFLFFGVVLFGGMM
                     ARIGAMVWGTPQDEIRRGEPWTVGHVPMLAMIVALLGLSVMLPESLRTLVTRAVHVIV
                     VR"
     gene            4924..>5267
                     /locus_tag="PLS35_10020"
     CDS             4924..>5267
                     /locus_tag="PLS35_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNI19044.1"
                     /translation="MPDGLTAAARVQEAFPQGIIQVGSMQGVPMLRLRKPDVPAVARY
                     IHTDPELRGSLSLLWAIDHRPREARDELWYLFTLAARKDWLLLTVDLAEEEREFASIT
                     PMVHAAKWYERE"
     assembly_gap    5268..5367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5368..6384
                     /locus_tag="PLS35_10025"
     CDS             <5368..6384
                     /locus_tag="PLS35_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNI19045.1"
                     /translation="SVAGEPILQLEVRHFWKHRGVEKLFEQQRLTEAVPLAERVSGDT
                     SVGHSLAYCQAVETLLGMTVPPRARYLRSLFLELERLHNHIGDVGAICNDTAYALAHA
                     HCGRLKEQLMQLNDGLTGSRFLRGVNRVGGVSIDLSGVQLSQVDAELQAIEQEFSGIE
                     KILLANASLTERLENTGVLSESIAWDHAVMGVVGRASGIDRDLRRDRPFAAYPECQPK
                     VAVYRYGDVRARMRVRLDEVHDSMRLIHTLRRQLPMGPLATQAVEPVTQGQWAISAVE
                     GWRGEILYVVMAGEEGRIHRCKVRDPSFVHWPAIQWAALGNIVPDFPLINKSFNLSYA
                     GNDL"
     gene            6441..6962
                     /gene="nuoB"
                     /locus_tag="PLS35_10030"
     CDS             6441..6962
                     /gene="nuoB"
                     /locus_tag="PLS35_10030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNI19046.1"
                     /translation="MFRIIKKSLTTGVVTGQYPMAKASQEPVSREAIEKVKPFRRSLT
                     IREVDTGSCNACEMEMNALANPVYDVERFGVEIAASPRHADALVVTGPVTVNMERALK
                     DVYKQTPDPKLVIALGDCAITCGMFKGSYAVTGPVDRHIPVDVRIAGCPPRPVEILAV
                     LAELRKDCPDSDQ"
     gene            7132..7401
                     /locus_tag="PLS35_10035"
     CDS             7132..7401
                     /locus_tag="PLS35_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19047.1"
                     /translation="MGKIFSEIHFLRFFTPFLLSARYLEHVVEEKGRAAMWLVGDGLE
                     RDSGEARLNTCAVSLRNIDMDLIYLAIAAVFFLLSGWLISALDRL"
     gene            7415..7504
                     /gene="kdpF"
                     /locus_tag="PLS35_10040"
     CDS             7415..7504
                     /gene="kdpF"
                     /locus_tag="PLS35_10040"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNI19048.1"
                     /translation="MNAMYVLGAVLSLGLLVYLMVALLKAEWF"
     gene            7504..9309
                     /gene="kdpA"
                     /locus_tag="PLS35_10045"
     CDS             7504..9309
                     /gene="kdpA"
                     /locus_tag="PLS35_10045"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNI19049.1"
                     /translation="MTINGLAQIGLFFLVLAALVKPLGWYMARVYTGQACGMDRVMGP
                     FERLIYRVCGVREAEAMNWTSYAVAMLLFNVAGLVALYALQRLQGLLPLNPSAFGAVA
                     PDLAFNTAVSFITNTNWQAYAGESTLSYLTQMLGLTVQNFVSAGTGMAVLVALIRGLT
                     ARTASTIGNFWVDLTRSTLYILLPLSAVLALVLVSQGTVQTFGLYHQTALLQPVTYDK
                     PVVDAAGQPVVDEKGVAKMESTTGIEQVLAVGPAASQVAIKHLGTNGGGFFNANAAHP
                     YESPTPLTDFILILAETLIAASLTYTFGMMVGDTRQGWTILAAMLSVLAFFVLGAYWA
                     ESAGNPRLTALGVEQNVQIAQSGGNMEGKETRFGVARSALFATVTTATSTGAVNSMHD
                     SFTPLGGLIPLLMMQFGEVILGGVGSGLYGMLVFAIIAVFIAGLMVGRTPEYLGKKIE
                     AYEMKMAALLILIMPIVVLGFTAVAVVTESGTSSILNAGAHGFSEILYAYTSQGNNNG
                     SAFGGLNANTPFYNVTGALAMLVSRFWLAIPTLALAGSLARKKMVPLGLGTLPTHTPL
                     FVVLLIGVVIMVGALTFVPALALGPVVEHLLMVAR"
     gene            9333..11444
                     /gene="kdpB"
                     /locus_tag="PLS35_10050"
     CDS             9333..11444
                     /gene="kdpB"
                     /locus_tag="PLS35_10050"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNI19050.1"
                     /translation="MTTSTKARPLFDPPLVRQALLDALRKLHPRIQVRNPVMFVVFVG
                     SLLTSLLFFQALFGAGEAPTWFILAISLWLWFTVLFANFSEAIAEGRGKAQADSLRKS
                     RTELTAKRFGTPASGGDYLAAPAAIGKPGEVYSLVPASLLKKGDIVLVEAGDYIPSDG
                     DVIEGIASVNESAITGESAPVIRESGDRSAVTGGTRVLSDWLVVRITTSAGETFLDRM
                     IAMVEGARRQKTPNEIALNILLAALTVVFLLATVTLLPFSLYSVHATGQGAPITVTVL
                     VALLVCLIPTTIGALLSAIGIAGMDRMVQANVIAMSGKAVEAAGDVDVLLLDKTGTIT
                     LGNRQATAFIPAEGVPETSVADAAQLASLADETPEGRSIVILAKERYGIRGRDIHAMG
                     ATFLPFTAQTKMSGVDLDDRQIRKGAAEAIEDYVTQQGGSFPPAVRDAVETIASQGGT
                     PLVVAEGKTVLGVIHLKDVVKGGIKERFAELRRMGIKTMMITGDNQKTAAAIAAEAGV
                     DDFLAQATPEAKLKLIRDLQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEA
                     GNLVDLDSNPTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIIPAAFATTYPALG
                     ALNVMGLATPESAVLSAVIFNALIIIALIPLALRGVKFRPIEASLLLRHNVLVYGLGG
                     IIVPFIGIKLIDLLLVALGLV"
     gene            11455..12036
                     /gene="kdpC"
                     /locus_tag="PLS35_10055"
     CDS             11455..12036
                     /gene="kdpC"
                     /locus_tag="PLS35_10055"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNI19051.1"
                     /translation="MAAQLRSALMVFFILTILTGVAYPLAVTGIAQLLFPHQANGSLI
                     YKDGKPIGSTLIGQPFDDPKYFWGRPSATAPFPYNASASSGSNLGPTNPALTEAVKTR
                     VAALKAADPRNDAPVPVDLVTASGSGLDPHISPAAAEYQMRRVARARGKDEAVVRTLV
                     IQHTEGRQLGLLGERRVNVLALNLALDASGQAR"
     gene            12122..14830
                     /locus_tag="PLS35_10060"
     CDS             12122..14830
                     /locus_tag="PLS35_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNI19052.1"
                     /translation="MENQRPDPDLLLRRVQAEEADQTRGRLKVFFGATAGVGKTYAML
                     QAAHEQQRDGIDVVIGWVETHGRAETEALVHGMPTLPPREVVYGGSRLRDFDLDAALA
                     RRPQLLLMDELAHTNAPGSRHPKRWQDVRELLQAGVHVYTTVNVQHIESLNDAVAQIT
                     GIRVSETVPDAVLEQADDVELVDLPPDDLLQRLKDGKVYLPDQAQHAIKHFFRKGNLI
                     ALREMALRRTAERVDQQMEVYRRDHAVVGTWPAAETILVCVNLKPRGPMLVRAARKMA
                     TGLHARWIAVYVQTSKHLRLSDQERNQGVDTLRLAERMGAETVTLTGEDVSAQLLAYA
                     RSRNATKIIVGKPLRSRWKEWLFGSVVDDLVRGSGDIDIYVMTGEGGTGKPLVKGLLQ
                     RTSERRNYGYASAVVGACTALSLVMSPYFGQANLIMVYLFGVVVVATRWGRGPSALAS
                     LMSVAAFDFFFIPPYYSFAVSDAQYLLTFAVMLLVALLISRLAAHKSQQAEAALIREK
                     RTAALYAMSRELITQRGVDKLASVASRHIHDVFHCQVAVFLPDREGRVHLHRGEALHF
                     ELDPKEAGISQWVFDHKESAGHGTNTLSGSDSLYLPLLGSHGAVGVLAVRSSRPAELL
                     APEQLHLLETFANQMALALERARLAEEAQEAHVQTETERMRNAVLSSVSHDLRTPLAT
                     IVGASSALLSTTRPLSTESQQDLVRSISDEAGRLDRLLKNLLDMTRLEAGAMHLRKEW
                     HALEELVGTALARVKSRIATHTVTTAFPVNLPLVLVDGVLIEQVLINLLENAAKYSPT
                     ASPIEVTASVKDGVILIEIADRGPGIPQGEERRIFEKFYQLHPEREGGVGLGLTICRG
                     IIESHGGRIWVDSRSGGGSSFRFTLPAEKSQPTVEPEANQHDSAAI"
     gene            14843..15550
                     /locus_tag="PLS35_10065"
     CDS             14843..15550
                     /locus_tag="PLS35_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19053.1"
                     /translation="MPSTTTHEVTVLLIEDEAEIRRFLRTTLPPYGYRLYEATTGADG
                     LAQISARNPDIILLDLGLPDMNGTAVIEKIRAWSNMPIIVLSARDQEQVKVQAFDLGA
                     DDYVTKPFGVDELLARMRAALRHAHRPIGDAVEPVCTFGEVTVDLGRRQVFVSGTEVH
                     LTPIEYKLLTTLVRYAGKVVTHRQLLKEVWGPLHVDEGHYVRVYMRQLRNKIEADPAQ
                     PRHLVTELGVGYRLRAE"
     gene            15673..>16063
                     /locus_tag="PLS35_10070"
     CDS             15673..>16063
                     /locus_tag="PLS35_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19054.1"
                     /translation="MEPILPLRNDIGLAVRPIFSAGMSRLGWVLFFWLLIAPAYAAEP
                     SSTPGDSPAVSADSGLWHYGAYLDVAYILNFNFPENHLWRNRATAARHNEFAPNMALA
                     YVRKDVNESSRWGMELGVQGGYDSERFA"
BASE COUNT         2917 a         4366 c         4986 g         3694 t
ORIGIN      
        1 catgacgctg gtggtcctgg ccgacaacgg gttcttcttc ctgatcgtct gggaagtgat
       61 gtccctgctc tcgtacgtcc tggtggtgac ggagcatgaa cggcccggcg tgcgtgaagc
      121 cgggttgttc tatctgatca tgacgcatgc ggggacggcc tttatcattt tgacgtttct
      181 gctcttttct caagaaaccg gctccttctc cttcgaggcg tttcggcatc cagaccagcg
      241 gttgccggac ggcctgcgat cgatcgcgtt tttcacggca ttgatcggat tcggcgcgaa
      301 ggccggtatt gtcccgctgc acgtgtggtt gccctacgcg catccggcgg cgccgtctca
      361 catctcagcg ttgatgtcag gcgtgatgat caagacggcg atctatggat taatccgggt
      421 gtactttgat tttttggagg gacagtttcc ctggtggtgg ggttttacgg tgttgagcat
      481 cggcgcggtg tctgcgctcc tgggcgttat gtacgcgctc atggaacacg acttgaaaag
      541 cctgctcgca ttttccagtg tagaaaacgt cgggatcatt ctgttgggta tcggcgcagg
      601 catgattttt cacacctatg gcctgcacga actggccgca ctcggattgt tggccggact
      661 ctaccacacc atcaaccacg cggctttcaa agggctcctg ttcctcgggg ccgggtcgtt
      721 gctgttcgtg acgcacacga gaaatatcga agagtacggc ggcctgctca gacggatgcc
      781 gtggaccggg ctctgtttcc tgattggtgc cgtgtcgatt gtggcgcttc cgcccgccaa
      841 cggcttcgtc agtgagtggc tggtctttca gagcctcttt ctcagcgtgc aattgccgac
      901 gttgtttctc aagctgatgt tgcccatcgc cgctgcaatc ctcgcgctca ccggcgcgct
      961 ggcgttggcc tgtttcgcaa aagccttcgg cctatctttt ctggcgcagc cgcgcagcgc
     1021 ccatgcccgg catgccgttg aagtgccggt gtcgatgcga atcggaatgg gcctgctggc
     1081 gtcgagttgc gccctgtttg ggttagcgcc catgctcgtg gttcccttgt tggatcgggt
     1141 gacgaccccc ttggcgggcg tggcgatcag cgaaaaagtg ttggcgctag acggctgggt
     1201 ggttgctcca gtgacggtgg aattctccag tatttcgatg ccggtcctcg cggcattgct
     1261 ggtcggcgcg gctgggttag ggctggcgat cgcccggctg ctgaggaaag gagtgcatgc
     1321 gcggtactcc aggacgtggg gttgtggcct tccgctcacg ccacgcatgg aatattccgc
     1381 aaccggcttc gtgcagccga tcaaacaggt gttcgagatg atctatcagc ccacggtgaa
     1441 gttagagcga gagtttctga agcagtcgaa gtacttcatc cggcatcagc gattccacag
     1501 tagtatcgaa cccctgttcg agaagtatgc gtatcgcccg atcgtcaacg ggctgttggc
     1561 ggccgcggag cgcctgcgcg ttatccaggc cggcagcctc catctctact tggcctacat
     1621 tttcgtgacg ctggtgctgt tgttgctctg ggccggatag ggtcagacag gtcatgaagt
     1681 tcacgctgcg gacccgtcag tgaataccag atgaggaacg acgtgcaggt gatatcccag
     1741 atcggattcg tcttcataca gctcatcata ctgctcgcat tttctccgtt catcgtagga
     1801 gtgatccgga aggtcaacgc gcgtcttcaa tgccgccgtg gtgcgagtct gttgcagccc
     1861 tatgccgatt tggccaagtt gttcggcaaa caaccggtga tttcctccac cacctcgtgg
     1921 atttttacgg ccgcgcccta catcgtgttt gcatcgacag tggcggccgg cctcctgatg
     1981 ccggtgttca tctcccacac gccgctcaac ttcgccggca acatcattgc gttggtgtac
     2041 ctattggcct tggggacgtt tttcttgatt ttggcgggat tggatgccgg atcggcgttt
     2101 ggcggaatgg ggagcagtcg ggaagccatt gtggtgaccc tggccgaacc agcgatgatt
     2161 ctgtccattc tggccattgc cttgacggct ggatccacca atttgagtac gatcgtccat
     2221 cagtcggcgt tgctggaagg catggtgctg gcgccgcccg cacacctcat ggcgctggcc
     2281 gcgatgttta tcgtgacctt ggcggagacc gggcgcgtgc cggtggacaa tccggcaacg
     2341 catctggaac tgacgatgat tcatgaggcc atgctgctcg aatattcggg ccggtacctg
     2401 gcgttgatgg aatgggccgc gggtatcaag ctgctggtgt tcctgacgtt gctcgtcaac
     2461 gtgttcgcgc cttgggggat cgcgacggag gcgacactat cagccctggc gactggcgtt
     2521 gcggtctatg tcgccaaagt aacggggttg gccgtcatca ttggcgtgat tgaatcgatg
     2581 ttcgcgaagt tgcggctgtt tcgtgtgccg gaactgctcg gttccgcttt cattctggcg
     2641 ctgctggcct tggtgttctt ctacacgttg cgagggtaag gcgatgtcgg aatcaacaca
     2701 gctcgggtcg cagctcgtcg atctctgctc ggtgttgctg ctgttgagtt gcttcgccat
     2761 cgtggcgcag cggcggctct cggcttgcgt cgatctgttc gcgctgcaat cgttcttttt
     2821 ggcgaccacg gcggcgctcg tcgccttcct gaccgggcat cgccacatct atctggccgc
     2881 ggcactgacc gtggtcatca aagtgatcgt gttgccgcgg attctccgaa aggtcatcga
     2941 gcgtctgaat gtgtcgcgag aactggtcat gaatatcaac attccggccg ggctgttgat
     3001 ctgcggcgga ttggtggtgg tggccttttt catcacgcag cctattattc cgctggggta
     3061 tctcctgact cgcgattcgc tggccctggc ccttgccatc attttgatcg gcctgtttac
     3121 gatgatcgcc aggaagaaag ccgtgacgca aatggtcgga tttctggtga tggagaacgg
     3181 cgtcttcctg ggaacgacgg cggcggccta cggcatgccg ttgatcgtcg agctgggcgt
     3241 attttttgat gtactcgtcg cgggcctgat tgcgggcatt tacacccatc ggctgcagga
     3301 tgcgttcgat agcgtggata ccagcgagtt aacggtcttg aaggaatgat gatccgatga
     3361 tggagaactc gcgaacaggt gtcccatgtg gccggtgatc gtgttgttgg cagggccggt
     3421 cttggccggg ctgctgagcc tggtgatcca acgtgcgcgg ctgctgcacg ccgtcaattt
     3481 tacgaccatg ctgggattag ccgtcgccga gaccttcctg accaaacggg tgttggcaga
     3541 aggatcggtc acgacgttgg actcgctcgt gtatatcgat gcgttgtcgg actttattct
     3601 catcatcatc acgaccatcg gactctcctg ctcgctgtac atgtggtcgt atatggatga
     3661 gcaggtcgcc cggggtgtga tcgcgccgaa gcacctgggg gtcttcttct tcctgtttca
     3721 tatgtttctg ttcgccatgg ttgccgcgac gatggccaac agcttaggcg tgcagtgggt
     3781 cgccgtggag gggaccacat tggcgacgac ctttctgatc gccttttttc gacgccggga
     3841 atcactcgaa gccggctgga aatatttgat tctctgctca gtggggatcg cccttgccct
     3901 gttcggggtc gtgctgacct attactcctc ggtgcgggtg cttggcgatg ccagttcggc
     3961 gttgaacgtg acagccctga tcggggtggc ggatcgattg gatccgaatg tcctgaagct
     4021 ggcgtttctc ttcattctcg tggggtacgg gacgaaggtc ggcttggtgc ccatgcatac
     4081 ctggttgccc gacgcctata cggaagcgcc ggcgccggtt gcggcgatgt tggccggggt
     4141 gttggaaacg gtagcggtgt atacggtgct gcgcagcaag gcgctcgtcg atcaggcact
     4201 cccgcctcag ttcaccggta atctgctgct cacgttcggg ttcgtgtcgt ttgtggtggc
     4261 ctcgctgttc attctcctac agcacaatta taagcggctg tttgcctatt cgagcatcga
     4321 gcacatgggg ctggccatgc tcggttttgg agtaggcggg ttggtcggta ccttcggagg
     4381 cctgttccat ttgctcaatc atgcgttagc caaggcgctg gcattttttg tcgcgggcaa
     4441 catccatcgg cggtacgact cgttggagat cgaccatgta cgagggcttg cgcgatcgca
     4501 accgatcacg gcactggcga ctcttgtagc cgggttcgcg ttggtcggcc tgccgccgtt
     4561 ttcgccattc gtgagtgaat tgctcgtggt atcggcgctc tcggcacaga attttgcatc
     4621 cgatacgatg catgtcggtc ggttcgtggc ggtgacggtt tccgatgaaa tgcggagtct
     4681 tggactcgtc ggcgtctttt tgtttttcgg cgtggtcctg ttcggtggca tgatggcgcg
     4741 cattggcgcg atggtctggg gcactccgca ggacgagatc cgccgaggag agccctggac
     4801 agtcggccat gtaccgatgt tggccatgat cgtggcgttg ctcggcttga gcgtcatgct
     4861 tcccgaatcg ttgcggacat tggtgactag agctgtccac gtcattgtgg tgaggtgaac
     4921 cctatgccgg acggactgac agcagccgcg cgagtgcagg aggcctttcc gcagggcatc
     4981 atccaagtcg gttcgatgca gggcgtgccc atgcttcgtc tccggaagcc agacgtaccc
     5041 gccgtcgcac gctatatcca taccgatccg gaattgcgcg gctcgctttc gctcctctgg
     5101 gccatcgatc atcgccctcg cgaagctcgc gatgaactct ggtacctatt cacgctggca
     5161 gcacgcaaag actggttgct cctgacggtt gatctcgcag aagaggagcg ggagtttgcg
     5221 tcgatcaccc cgatggtcca tgcggcgaag tggtatgaac gggagatnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gtagccgggg aaccgatcct ccagctcgaa
     5401 gtgcgtcatt tctggaagca ccgcggggtg gaaaaactat tcgagcaaca gcgcctcacg
     5461 gaggctgtgc cacttgcgga gcgcgtctcc ggcgatacct cggtcggcca tagcctcgcc
     5521 tactgtcagg cggtggaaac actcttggga atgacggtgc cgccgcgagc ccgctatctg
     5581 cggagtctct ttctggagct ggaacggttg cacaaccaca tcggcgatgt cggcgccatc
     5641 tgcaacgata cggcctatgc gctggcacat gcgcactgtg gccgtctgaa ggaacagctc
     5701 atgcagttga atgatggcct gaccgggtca cgctttcttc gaggggtgaa cagggtcgga
     5761 ggagtgtcga tagatctttc cggtgtgcaa ctgtcgcagg tcgacgcaga attgcaagcc
     5821 attgaacagg agttttccgg aattgaaaag attctgctgg cgaatgcctc tctgacggaa
     5881 cgattggaga acacgggagt gttgtcggaa agtatcgcgt gggatcatgc ggtcatggga
     5941 gtcgtgggcc gcgcttccgg tatcgatcgg gatttgcgtc gggatcggcc gttcgccgcg
     6001 tatcccgagt gtcaaccgaa ggtcgcggtg tatcgctacg gggatgtccg cgcgcgcatg
     6061 cgggtgcggt tggatgaggt gcatgattcg atgcgtctca tccatacgct tcgccgacaa
     6121 ctgccgatgg ggccgctggc cacccaggcg gtcgagcctg tgacgcaggg gcaatgggcg
     6181 atatctgccg tcgaagggtg gcgtggtgaa attctgtatg tcgtgatggc aggggaagag
     6241 ggacggattc accggtgtaa agtgcgggat ccatcgtttg tgcattggcc ggcaattcag
     6301 tgggcggcgc tggggaacat cgtgccggat tttccgttga tcaataagag cttcaatttg
     6361 tcctatgccg gcaatgatct gtagcgggaa gctgaaggcg actgactgcg cgattgtccc
     6421 gacgatctca cggaggtgcc atgtttcgaa tcattaagaa aagtcttacg actggcgtcg
     6481 tcacgggcca atatcctatg gcgaaggcgt cacaagagcc ggtgagccgg gaggcgattg
     6541 aaaaggtcaa gccattcagg cgatccctca cgattcgaga ggtggatacc gggtcgtgca
     6601 acgcctgtga gatggaaatg aatgcgctgg ccaatcccgt ctatgacgtg gagcgcttcg
     6661 gggtggagat cgctgcgtcg ccgcgacacg ctgatgcgct ggtggtgaca gggccggtga
     6721 ccgtcaacat ggagcgcgcc ttaaaggatg tgtataagca gacgcccgat ccgaagctcg
     6781 tcattgcgct gggtgattgc gcgatcactt gcggaatgtt caaggggagt tatgcggtga
     6841 cgggtccggt agatcgccac attccggtcg atgtccgtat tgccggttgt cctccacggc
     6901 cggtcgaaat tctggcggtg ttggctgagc tacgtaaaga ttgcccggat tcagatcagt
     6961 agtgtcctga tgcagtcacg cctctctagt tgctccaaca attctctgtg acctgatcaa
     7021 tagacggtca cggctttgtc cacatgtctt ccttagcacc ccgctcgtaa gtggatgtat
     7081 tacgagtgat cagtcgtctg tagcgatctc ctccgaaccc gtgaacagct gttggggaag
     7141 atcttctcag aaatccattt ccttcgcttt tttacaccgt ttttactttc tgcgcgctac
     7201 cttgaacatg tggtggaaga gaaggggcgt gcagcgatgt ggctggttgg cgacggcttg
     7261 gaaagagact ccggcgaagc gcgcctcaat acgtgtgccg tctcattaag gaacattgac
     7321 atggatctca tctatttagc gatcgctgct gtgttttttc tgctgtccgg ctggcttatc
     7381 tcggcgttgg accgactcta aagggggtct ggtcatgaat gcgatgtatg tcctcggcgc
     7441 agtgttgtcg ctgggtttgt tggtgtacct catggtcgcg ctgctgaaag cggagtggtt
     7501 ctaatgacga tcaacgggtt ggctcaaatc ggactgttct ttctcgtttt ggcggcgttg
     7561 gttaaaccgc tgggctggta tatggcgcgt gtctacacgg gccaggcctg cggaatggat
     7621 cgggtgatgg gacccttcga acgcctgatc taccgtgtct gtggcgtgcg cgaagccgag
     7681 gccatgaact ggacgagcta tgcggtggcc atgttgttgt tcaatgtcgc cggattggtc
     7741 gcactctatg ccttgcagcg actgcaggga ttgctgccgc tcaatccgtc ggctttcggg
     7801 gcggtggcgc cagacctcgc attcaatact gccgtcagct tcattaccaa tacgaactgg
     7861 caggcctatg ccggtgaatc gacactcagt tatctcacgc aaatgctcgg cctgacagtt
     7921 cagaatttcg tctccgccgg cacgggcatg gccgtgctgg tcgcgctcat ccgcgggtta
     7981 accgctcgga cggcctcgac catcggcaat ttctgggtgg acctgactcg cagcacgttg
     8041 tacatcttgc tgccgctgtc agccgttctc gcgctggttc tcgtctcgca agggacggtc
     8101 cagaccttcg ggttgtatca ccaaaccgct ctcctccagc ctgtgaccta tgacaagccc
     8161 gtcgtcgatg ccgcaggtca gccggtagtg gacgagaagg gcgttgccaa aatggaatca
     8221 acaaccggga ttgagcaagt cttggccgtc ggtccggcag cgtcccaagt ggctatcaaa
     8281 catctgggga ccaacggtgg cggattcttt aatgccaatg cggcccatcc ctatgaaagc
     8341 ccgacgccgc tcaccgattt cattctgatc ctggctgaaa ccctgatcgc cgcatcgttg
     8401 acctatacgt ttggaatgat ggtcggcgac actaggcagg gatggaccat cctcgctgcg
     8461 atgctgagcg tattggcctt cttcgtgctg ggggcctact gggcggagtc cgcaggaaac
     8521 cctcgcctga ctgcgttggg tgtcgagcag aacgtccaga tagcgcagtc cggtggcaac
     8581 atggaaggca aggaaactcg cttcggagtt gctcgttcgg cgctttttgc caccgtaacg
     8641 acagccacgt ccaccggggc ggtgaattcc atgcacgatt cctttacgcc actcggcggt
     8701 ttgatcccgc tgctcatgat gcaattcggc gaggtcattc tgggcggggt agggtccggc
     8761 ctctacggaa tgctggtttt cgccattatc gcggtgttca tcgccggtct gatggtagga
     8821 cggaccccgg agtacctggg gaaaaagatc gaggcctatg aaatgaagat ggccgcgctg
     8881 ttgatcctca tcatgccgat cgttgtcctg gggttcacgg cagtggcggt cgtgactgag
     8941 tccggcacgt cgtccattct caatgccggc gcacatgggt tcagtgaaat tctgtatgcc
     9001 tatacctccc agggcaataa caacgggagt gccttcgggg gactgaatgc caataccccc
     9061 ttctacaacg tgacgggcgc attggccatg ctggtctcca gattctggtt ggcgattccg
     9121 acattggcgc tcgccggatc gttggcgcgc aaaaagatgg tgccgctggg gttgggcacg
     9181 ctcccgactc atacgccgct cttcgtggtc ttgctgatag gggtcgtcat catggtcggc
     9241 gccctgacct tcgttccggc cctggcgttg gggccggtgg tggaacattt gctgatggtc
     9301 gctcgctagc ctgaacgagc agagggattt ccatgacgac atcgacgaaa gccagaccgc
     9361 tcttcgatcc gccgcttgtt cgacaggccc tgctggatgc gctacgaaag ctccatcctc
     9421 gcattcaggt ccgcaatccg gtcatgttcg tggtcttcgt gggaagcctc ttgacgtcgc
     9481 tgctgttctt ccaggcgttg ttcggggcgg gcgaagcacc gacctggttt atcctcgcca
     9541 tctcgctgtg gctctggttc accgtgctgt tcgccaattt ttcagaagcg attgctgaag
     9601 gaagaggaaa ggcccaagcc gattcgctcc gcaaatcgag gacggagttg accgccaagc
     9661 gatttggaac acctgcctcc gggggagact acctggcggc gcctgccgcc atcggcaaac
     9721 cgggagaggt ctacagtctc gttccagcca gtctgctgaa gaaaggtgac atcgtgctgg
     9781 tcgaggcggg ggattacatt cccagcgatg gagatgtgat cgaggggatc gcatccgtca
     9841 atgaaagtgc gatcaccggc gagagcgcgc cggtgatcag ggagtccggt gaccggagtg
     9901 ccgtgaccgg cggcacgcgg gtgctgtcag attggcttgt cgtgcgaatt accaccagcg
     9961 ccggtgaaac atttctcgat cgtatgatcg ccatggtcga gggtgccagg cgccagaaga
    10021 cgccgaatga aatcgcgctc aatattctct tggcggcgct caccgtcgtc tttctgctgg
    10081 cgacggtgac cttgctgccg ttttctctct attccgtgca tgctacgggg cagggggcgc
    10141 cgattacggt cacggtgctg gtggccctgc tggtttgtct cataccgacg acgatcggcg
    10201 ctctcttatc cgccattggc atcgcgggca tggaccgcat ggtccaggcg aacgtcatcg
    10261 ccatgtcggg gaaagccgtc gaagcggcgg gcgatgtgga cgtgttactc ctcgacaaga
    10321 cgggaacgat cacgctgggc aaccggcagg ccacggcatt tattccagcc gaaggcgtgc
    10381 cggaaacgtc tgtggccgat gcggcgcagc tggcctcgct agccgatgag acgcccgaag
    10441 gccgcagcat cgtgattctg gcaaaggaac gctatggtat cagagggcgc gatatccatg
    10501 cgatgggcgc caccttcctc ccgttcacgg cgcaaacgaa aatgagcggc gtcgatctgg
    10561 acgaccgaca aattaggaag ggcgcggcgg aggccatcga agactacgtg acccaacaag
    10621 gcggctcgtt tcctcccgcc gtgcgcgatg cagtcgagac gattgcctcg caaggcggta
    10681 ctcctctggt ggtggccgaa ggcaagaccg tgctcggtgt cattcatttg aaggacgtgg
    10741 tcaagggagg catcaaggaa cggtttgccg agttgcgccg catgggcatc aaaacgatga
    10801 tgatcaccgg cgacaaccag aaaacggcgg ccgcaatcgc cgcagaggcc ggcgtggatg
    10861 attttctcgc gcaggccact cccgaagcca aattgaaact catccgtgat cttcaggcag
    10921 gaggccgact cgtggccatg acgggcgacg gcaccaatga cgcgccggcc ctggcgcagg
    10981 ccgatgtggc cgtcgccatg aataccggca cccaggccgc caaagaagcg gggaaccttg
    11041 tcgatctgga ttccaaccct accaaattga tcgagatcgt ggaaatcggc aagcagctcc
    11101 tcatgacgcg gggggccctt acgaccttca gtatcgccaa cgatgtcgcg aagtattttg
    11161 cgattattcc cgccgcgttt gccacgacgt atccggccct gggcgcgctg aatgtcatgg
    11221 gactggcaac gccggaaagc gccgtcttgt cggcggtgat tttcaatgcc ttaatcatca
    11281 tcgcgctgat tccgctcgcg ctgcgagggg tcaagttccg gccgattgag gcctccttgc
    11341 tgctgcgcca caatgtgctg gtgtacggac tggggggcat cattgttccg ttcattggaa
    11401 tcaaactgat agacctgctc ctggtcgccc tgggactggt ctgaggagat cacgatggcc
    11461 gctcaacttc gatccgcgct gatggtgttt ttcatcctga cgattctgac cggtgtggcc
    11521 tatccgctgg ccgtgacagg gatcgcgcag ctgctgttcc cccatcaggc caatggcagt
    11581 ctgatctata aagacggcaa gccgatcggg tccacgctga tcggccaacc cttcgacgat
    11641 cccaagtatt tctggggacg cccttccgcg accgctccgt tcccctataa tgcctccgcc
    11701 tcctccgggt cgaatctggg ccctaccaat cccgcgctga cggaggcggt caagacgagg
    11761 gtggctgcgt tgaaggctgc ggacccccgc aatgatgcac cggtcccggt cgatctggtg
    11821 accgcgtcgg gaagcggcct tgacccgcat atcagccccg ccgcggcgga gtaccaaatg
    11881 cgccgggtcg ctcgtgcacg aggaaaagac gaagcagtcg tgaggacact ggtcatccaa
    11941 catacggaag ggcgtcagct gggactgctc ggtgaacgcc gggtcaatgt gctggcgttg
    12001 aacctcgcat tggatgcatc cggccaggct cgttgacgcc tttccgtctg tcttctagaa
    12061 tttgctctgc caatcttgtg gccttttcgt cccccatcga atttctttgt ccaggagtgc
    12121 catggagaac cagcgtccgg atcccgatct actgctgagg cgcgtgcagg ctgaagaagc
    12181 cgatcagacg cgcggcagac tgaaggtctt tttcggcgcg acagcagggg tcgggaaaac
    12241 ctacgccatg ctgcaggcag cccatgagca gcaacgggac ggcatcgatg tggtgatcgg
    12301 gtgggttgag actcatggcc gggccgaaac ggaagcgctc gtccatggga tgccgactct
    12361 gcctccccgg gaggtggtct acgggggatc aaggcttcgg gatttcgatc tcgacgccgc
    12421 tctggcgcgc cggccgcagc tcctcttaat ggacgaactg gcgcatacga atgcccccgg
    12481 ttccagacat cccaagcggt ggcaggatgt gagggaactc cttcaagcag gcgttcacgt
    12541 ctataccacc gtgaacgtcc agcacatcga aagcctcaac gacgcagtag ctcaaatcac
    12601 cggcattcgt gtgagtgaaa ccgtgccgga tgccgttctt gagcaggctg acgacgtcga
    12661 attggtagat ctccctccgg acgatctcct ccaacggctc aaggacggca aggtctatct
    12721 gccggatcag gcacagcacg ccatcaagca tttttttcgg aagggtaatt tgatcgcgtt
    12781 gcgggagatg gccttgcgtc ggacggccga gcgtgtcgat caacaaatgg aagtgtatcg
    12841 tcgggaccat gcggtcgttg gcacctggcc ggcagcggaa accatcttgg tttgcgtgaa
    12901 tctgaaaccc cgtgggccga tgctggttcg cgcggccagg aagatggcca cgggcctcca
    12961 tgccaggtgg atcgctgtct acgtccagac ctcaaagcat ctgcggttgt ctgatcagga
    13021 gcggaatcaa ggtgtcgaca ccttgcggtt ggccgagcgg atgggcgcag aaacggtgac
    13081 gttgacgggg gaggacgtga gtgcgcagtt actggcgtat gccagaagcc gcaatgccac
    13141 caagattatt gtcggaaaac cattgcgttc gcgctggaag gaatggctgt tcggttcggt
    13201 tgtggatgat ctcgttcgcg ggagcggcga catcgatatc tatgtgatga ccggtgaggg
    13261 gggtactggc aaacctcttg tgaaaggatt gcttcaacgg accagtgagc gccggaacta
    13321 cggctacgcc tccgcggttg tgggagcctg cacggcattg agtctggtca tgtccccata
    13381 cttcgggcag gctaatctca ttatggtgta tctgtttggg gtggtggtcg ttgccaccag
    13441 atggggccga ggcccctcag cgctggcttc actgatgagc gtcgcggcct tcgatttctt
    13501 tttcatcccg ccctactatt cattcgccgt atcggacgcg cagtatctgc tgacatttgc
    13561 agtcatgctt ctagtagcgc tgctgatcag ccgactcgct gcccacaaga gccagcaggc
    13621 cgaggcggcc ttgattcgtg agaagcgaac cgccgcactc tatgccatga gcagggaatt
    13681 gatcactcaa cgtggggtgg acaaactggc ttcagtggcg tcccgccata tccacgacgt
    13741 ctttcattgt caggtggccg tgttccttcc tgatcgggaa gggcgggtgc atcttcatcg
    13801 gggtgaagcg ctgcatttcg agctggatcc gaaggaagcc ggcatctcgc aatgggtgtt
    13861 cgaccataaa gaatccgccg gtcacggcac caataccctg tccggatctg attcgctgta
    13921 tctgccgttg ttggggtctc atggggccgt gggagtcctt gcggtccgat cgtctcgacc
    13981 tgccgaactg ctcgctccgg aacaactgca tctcctggag accttcgcaa atcagatggc
    14041 gttggcgctg gaacgtgcgc gattagccga agaagctcaa gaagctcacg tgcagacgga
    14101 aacggagcgc atgcgcaatg ccgtcctgag ttctgtttct catgaccttc ggacgccact
    14161 ggcgacgatc gtgggcgcga gcagtgcctt actgagcacg acccggcccc tatcgaccga
    14221 gagtcagcag gacctggtcc gttccattag cgatgaagcc ggacgactcg accggttgct
    14281 gaagaatctt ctcgatatga cgagattgga agctggagcc atgcatctgc gcaaggaatg
    14341 gcatgcgctg gaggagctgg tgggcacggc attggcccgg gtgaaatccc ggatcgccac
    14401 gcatacggtc acgaccgcct ttccggtcaa tcttcccctg gtgctcgtcg atggggtgtt
    14461 gatcgaacag gtgctgatta atctgttgga gaatgcggcg aaatattctc cgacggcctc
    14521 tccgattgag gtgacggcgt cggtgaagga cggggtgatt ctgatagaaa ttgctgatcg
    14581 aggtcccgga attcctcaag gcgaagagcg gcggatcttc gaaaagttct atcagcttca
    14641 tcctgagcgt gagggaggcg tcggactggg attgacgatc tgccggggca tcatcgaatc
    14701 ccacgggggg cgtatttggg tagactctcg ttccggtggc ggatcgagtt tccgctttac
    14761 gctgccggca gaaaaaagtc agccgaccgt cgagccagaa gcgaaccagc acgacagcgc
    14821 ggccatttaa gatctgtcat ccatgccatc cacgacgacc catgaggtaa ccgtcctgct
    14881 catcgaggat gaagcagaaa ttcgacgatt tcttcgcacg actctcccgc cttatggtta
    14941 ccgattgtac gaagcgacca ccggtgcgga tgggttggcg caaatctccg ctcgaaatcc
    15001 tgacatcatt ctcctcgatc tcggtcttcc cgacatgaac ggcaccgcag tgatcgagaa
    15061 aattcgtgcc tggtcgaaca tgccgatcat cgtgctctcc gcacgagacc aggaacaggt
    15121 caaggtgcag gccttcgatc ttggcgccga tgattacgtc acgaaaccgt ttggcgtgga
    15181 tgaattgctg gcccgtatgc gcgcggcgtt gcggcatgcc catcgcccga tcggtgacgc
    15241 ggttgagccg gtatgtacgt tcggcgaggt gaccgtcgat ctcgggcgaa ggcaagtgtt
    15301 tgtctcaggg acggaagtcc acctcacgcc gattgaatat aagttgctca cgacgctcgt
    15361 gcggtatgcc gggaaggtcg tgacacatcg ccagttgctc aaagaagtgt gggggcccct
    15421 ccatgtagac gaaggacact atgtgcgtgt gtatatgcgt caattgagga ataagatcga
    15481 ggccgacccc gctcagcctc gtcatctcgt gacggaactg ggggtcggct atcgactccg
    15541 ggcggaatga gtattccggc cgtttttacg gatctcttac atctccatga tcggttttaa
    15601 tgctgttttg atacgaaatg ccgtagagtc gatttccgga gggcgcgaag tttgttggat
    15661 catgagcatg agatggaacc tattctgccg ttgcgcaacg acatcggtct ggctgtgagg
    15721 cctatctttt cggccgggat gagtcggctc gggtgggtgc tgttcttttg gttgttgatc
    15781 gccccggcgt atgccgccga accaagttcg acgcccggtg atagtccggc tgtcagcgcc
    15841 gatagcgggc tctggcacta cggcgcgtat ctcgatgttg cctacattct gaatttcaat
    15901 tttccggaaa accatctctg gcgaaaccgc gccacggcgg cgcggcataa tgagttcgcg
    15961 ccgaacatgg ccctcgccta tgttcgcaaa gatgtgaatg aatcttcacg gtggggcatg
    16021 gaactcggcg tgcaaggagg gtacgactct gagcgctttg cat
//
LOCUS       DAOQEW010000079        15847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1002_length_15847_cov_8.964222, whole genome shotgun sequence.
ACCESSION   DAOQEW010000079 DAOQEW010000000
VERSION     DAOQEW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_15847_cov_8.964222"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLS35_10075"
     CDS             complement(<1..634)
                     /locus_tag="PLS35_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19055.1"
                     /translation="MNTLVSSVLGRTDSDVLAWDTYVLNSPITSGYHLAGWRRVIEEA
                     FGHRTYYLIARDEHGAVQGIVPLVLLASRVFGRFLVSLPFVNYGGLVATSPDAYRVLE
                     GGAIEQANALDAAHIELRHERPIETGWVSTERKVSMRLSLPGSYESLVKAFPSKLRSQ
                     VRRAQKEGMTARIGGNECLDEFYAVFSRCMRDLGTPVYAKGFFAKILEVFP"
     gene            complement(642..2015)
                     /locus_tag="PLS35_10080"
     CDS             complement(642..2015)
                     /locus_tag="PLS35_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI19056.1"
                     /translation="MKRVLMVAYYFPPVAASGAMRPLGFCRNLAAYGWQPQVLTTTPE
                     CVYPSHQVDLKLGERVPADVEVIRVPYIDRLQRVLQYREMLKGMVRRGPAHKSASNPG
                     AAVRPPDSSPGRSTLKDFILDWAFAFPDRQCGWLSRAVRKLQTLHEKDVPHVIFATGG
                     PWTNFLIGDTLAKHFHCPLVLDYRDPWNCNPYYSFNSDALTRKSRATEAQVCQGASHV
                     IANTDELRERLIEEYEFLRDRCTWIPNGFDRETLSPPHVPQERSGLGLTSSGYELCHF
                     GTVYGKRTPRILLQAVWELFQENQLKPEAIRLRFVGGWDTTDQECEYYAANLEKYGFL
                     RREPPVSHSVCLREMQRSSVLLVLQPDSPLQVPAKIYEYVATGRPLLLIGGEGATANL
                     VARHGLGVSSPNHIARIKALLKDLTTGTQRLVPPDAARVNRFEYRSLTGELAAILDAA
                     VRPRPVG"
     gene            complement(2151..4037)
                     /gene="asnB"
                     /locus_tag="PLS35_10085"
     CDS             complement(2151..4037)
                     /gene="asnB"
                     /locus_tag="PLS35_10085"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNI19057.1"
                     /translation="MCGIAVAIGLNGRPVERAAVERMAKSLLHRGPDDGGIYMDGAVG
                     MGFRRLSILDLSEAGHQPMVSQDGQYVLVFNGEIFNYVELRDELRKLGHQFRSSGDSE
                     VLLAAYREWGRECLPKLNGMWAFVIYDRRHRRLFGSRDRFGVKPLYYSRDHTVIQFAS
                     EIKALRASGYQRVGINWRIASRFLLEGRLDSHNETFYDGIEQIPPGSGFEVGLDGAWH
                     QWTFWTLDALPKTIAPDPAATFGDLFEDSVRIRMRSDVPVGVCLSGGLDSTAIICAAA
                     RHRDESAGTQPESLQAFCYMAKEFDESKYIADTLLQTHAQLRQLETSPAELWNDVRKV
                     LWFQDEPVHTMTAVVGYQLMRLAASQGIRVVLNGQGADETIGGYSSYFQDYWVSLLQQ
                     GRVGAAWQAVKAYTEAHGGNSRARMTAAASRCISWEMYKIGSYRTWAQARRQARIRQN
                     PWFSGDLTKHFISEDTPPSSAALSTALKQSVISAPLPLYLRIEDRNSMAHSVEARLPF
                     LDYRLVSFVCGLSDEWKVRGPWNKYVLREGMKGRIPESVRSRVDKMGFPTASKKWFAH
                     DLYEPLREILASRTLRERGIYNTGALIADLDRHRRGEADFSNRLFHVAEFEILSDLAG
                     QSPA"
     gene            complement(4090..5100)
                     /locus_tag="PLS35_10090"
     CDS             complement(4090..5100)
                     /locus_tag="PLS35_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI19058.1"
                     /translation="MRILITGGAGFLGSHLSELLIGQGHDVIALDNLITGRAENIAHL
                     IGNPKFSFVKYNVCDYLHVDGQLDAVMHFASPASPQDYLEMPIATLKVGALGTHKALG
                     LAKAKGARFLLASTSEVYGDPLLNPQPETYWGNVNPIGARGVYDEAKRFAEAMTMAYH
                     RYHGVDTRIVRIFNTYGPRMRPKDGRVVSNFIVQALQGKPLTVFGDGSQTRSFCYVDD
                     LVRGIVALLMVKSDKSLAERTDRTKFLTQKPDAIFDSIHDPVNIGNPRELTVRGIAEI
                     ILKLTGSKSVIEERPLPADDPKVRRPDITRAKSLLGWEPKVELEDGIRKATEYFRQVV
                     VK"
     gene            complement(5135..6979)
                     /locus_tag="PLS35_10095"
     CDS             complement(5135..6979)
                     /locus_tag="PLS35_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HNI19059.1"
                     /translation="MNILGISAFYHDSAACLVRDGEIIAAAQEERFTRKKHDPGFPHK
                     AVEYCLQAGNISLKDVRHLVFYDKPLVKFERLLETYLAFAPRGVQSFVAAMPVWLKEK
                     LLLKSLLQKEFLVHAPELTTASLPQILFSEHHESHAASAFFPSPYDKAVVLCMDGVGE
                     WATTSAWLGEGNSLKPLWEIPFPHSIGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPK
                     YVKAIYEHLLDVKPDGTFRLNMDYFNYCTGLTMTGNKFDKVFGGPPRKPESKLTQREM
                     DLARSVQEVTEEVMLRLSRTLHRETGADFMCMAGGVALNCVGNGRILREGPFKGLWIQ
                     PAAGDAGGALGAALTTWYQYEQQPRKVVPGKDSIKGSYLGPSHTNEEIESYLQKAAAP
                     YVRLDEDHLYARVAEELAAGKVVGWLQGRMEFGPRALGGRSILGDARNRDMQSVMNLK
                     IKYRESFRPFAPSVLRERVSDYFVLDSDSPYMLLVAPVVEKRRLPVSSAQNGLWGIEL
                     LNIPRSDIPAVTHLDYSARVQTVHQDTNPRYYALLKAFEAKTGHGVLVNTSFNVRGEP
                     IVSTPEDAYRCFMRTEMDVLVLENCVLLKADQKPLEGDSDWKKEFELD"
     gene            complement(7103..8266)
                     /locus_tag="PLS35_10100"
     CDS             complement(7103..8266)
                     /locus_tag="PLS35_10100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI19060.1"
                     /translation="MNILLVTPWRPSLTGGISTVVARLSEEFRKKGHRVTIFVADREN
                     RLSQIEVLKDTPVYGMYLRSPVSSDHPIRAAIMYYVWFPLTVMQLLWLARRSQLDAIL
                     IQYPLPAMYYFGILKRLWTGILFVTYQGNDAHDLSSWSPQEQRLVRSLLEKADMVLGV
                     SRTLLSKVESAFQGIQLKRRQLIPNGAPLDVIEAVNGGSADTSLPQQYLLTAGHLIHR
                     KGIDIVIASLAKAKQRGVCLHLVVAGDGPERENLLRQSSELGLADQVIFLGNQTHEQV
                     LRLMKWCQAFVLASRAEGMPLVIAEAMACGKAVIASNVDGVPEIVHDGTTGILVPPDN
                     PEALASGLIRLCTDPVLRETLAMRGKEWACREYNWEGIASRYLSCIHECQSAS"
     gene            complement(8320..9249)
                     /locus_tag="PLS35_10105"
     CDS             complement(8320..9249)
                     /locus_tag="PLS35_10105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI19061.1"
                     /translation="MPRVSVVIPTFNCERFIGRTVDSALRQTYRDFEIIVVDDGSTDG
                     TQAVLEQYGDALHYIRQANQGASAARNAALNTTTGEYIAYLDADDLWEPQKLARQVAY
                     LDANPSCGFVHTEVAVIDEEDRVLHARFNKETKRTVPQGLCLRDILQRSHIQTLTVLE
                     RRSAFERAGKFDLRLPVAQDYLHWIQVVLQGYEIGYLPEPLGQYRWRAGSLMSSQRRL
                     LGDFVKIYEILLGEHRIGEVHGNEIRQLIDTQLYANQRQLAYVERLECSGAVARRRLR
                     RMVRQWPFHLELYLDLAKSYLSNPKVQMPVSPS"
     gene            complement(9274..10512)
                     /locus_tag="PLS35_10110"
     CDS             complement(9274..10512)
                     /locus_tag="PLS35_10110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI19062.1"
                     /translation="MKVLLISGAYPPMHAGEATNAYHLCNQLVERGIEVHVLTSVGNV
                     GTADPRIHVHPIMQGWGWTEWPRVRTFLKSCKPDAVFLMYIGLMYKFHPMVTFLPTLC
                     KKLFPNVPFVTRYESAFVGADPSKTGLVSRVIRKLMVRWAGVRDVVYSSGTLLRDSDS
                     VIALCERHRAMLIEEWPPVGGKVALIPPPPNLFIASSDGGEARERGRKRLGVGPTEFV
                     VTFFGYLYPIKGIETLLRAFATVSAQRPEARLLFIGGKVDLDVQGGASYFDEMQALAK
                     TLQIDGRTTWTGAFKSNEEDASLLLHASDVCVLPFLEGVQLNNSSFASMVAHGLPIIV
                     TRGPLMDQAFIHGENVLTCEPKDHQAVARLLLNVMDDAVLRDRLRAGALKLAADWFSW
                     ETATEKTLTALRPRLSAKVT"
     gene            complement(10553..11623)
                     /locus_tag="PLS35_10115"
     CDS             complement(10553..11623)
                     /locus_tag="PLS35_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNI19063.1"
                     /translation="MKQMLVTGSSGLIGSEVCAYFHGQGWMIHGVDNNTRATFFGPQG
                     DTRWNQHRLQADLKNFRHHELDIRNRQGALALIQELKPDVIVHTAAQPSHDLAAKIPF
                     DDFDTNAVGTLNLLEATRLHTPETVFVHMSTNKVYGDAPNELALIEQAKRWDYASPND
                     FDGIAEHMRIDQSKHSLFGASKVAADIMVQEYGRYFGMKTCCLRGGCLTGPNHSGVEL
                     HGFLSYLVKCNLEGKKYSVFGYKGKQVRDNIHSYDVARFIHAFIEKPRSGEVYNLGGG
                     RGNSCSILEAFEMIELVSGKKMLYEYVEKNREGDHICYISNLKKMTSHYPTWGITKTL
                     SHIFEEIHQSWVRRTVTMNVSH"
     gene            complement(11875..12753)
                     /locus_tag="PLS35_10120"
     CDS             complement(11875..12753)
                     /locus_tag="PLS35_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNI19064.1"
                     /translation="MTSRKPFGQILCEPNERVPVSQKKVAVVVPMHNRSTLTVDEQIS
                     FRHLTHYLAPYDKYLVVPESLQVTLPGCNLKRFGEEYFGSAIANTRLLLSEHFYRAFS
                     EYEYILIYHLDALVFSDQLTTWCDTGLDYIGPPWIPCVDSPWVKEPRVGNGGLSLRKI
                     QSFLKVFHSDEYWMDPEEYWRDKYAGMPTYLRWINSPKRLIKRLSLFNNARLEMSRWH
                     LRPDGTKNEDHFWSDRATHYVPDFKVASVEIGLRFAFEVAPRLCYDMNHRRLPFGCHA
                     WPRYDRGFWEPHLLVS"
     gene            complement(12814..14265)
                     /locus_tag="PLS35_10125"
     CDS             complement(12814..14265)
                     /locus_tag="PLS35_10125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNI19065.1"
                     /translation="MSSIGHRLGKFASRVLLFGVAVCLLLFAVMVWRHGMSIRYAVFL
                     LLALVFALGLRMPDTYKINSVLCGMSLAIAVYGVELLLAYSGSAIAALGAQAWLSFPQ
                     DANPRVAAERMKTVQETQQHFDTRSRLEVIQDMRARGINAFPDVFPMVLFEPGSGVSI
                     QSSLTINGEEFLPVAGMATTTTVFCNESGEYVVYESDEHGFHNPRGIWANQSAEIVAL
                     GDSYTHGVCVSSDKGFVAVVRSRHSGTINLGVNGHGPMTSLATLKEYGPILKPKHVLW
                     FYYEGNDLRDLDGWEKNSPLLRKYLTSSFSQRLFSRQAEIDQKLRAFLDTAMAKAATP
                     VSFEKVVKLQHLRQAVQLFYERRPTEQGLPAELLEYLRHTGAPAAPEDLQLFGTILAE
                     AQATASAWNGRVVFVYLPTWERYRIPETASQDRDKVLKIVERLQMPVIDMHPVFNAQP
                     DPLALFPFRRYAHYNEAGHKLVGEEVIRQLAKL"
     gene            complement(14369..15577)
                     /locus_tag="PLS35_10130"
     CDS             complement(14369..15577)
                     /locus_tag="PLS35_10130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI19066.1"
                     /translation="MLKQMRRYLQRYQWYLWMRELVPALCGVRRQFHGGVVSLHAAGN
                     PHGNVLVSYDNGGLLSKMRGQPIPTSHPQYYKTMVMAQTFVEIGYDVDVIHCENHKFV
                     PWKSYDVVVDTRFNLQRLAPYLPSNCIKILHCDTAQLVYQNVAEMTRVLAFQARRGVT
                     LPLNRLETPHLGVEQADYLTTCGNEFTMNTFAYSGKPIFRLPMLAQKMWPWPETKDFE
                     ASRHRFLWFGSRGMVHKGLDVVLEAFAQVPECQLTIVGPVHDEPEFVNVYRKELFHTS
                     NIKCVGWLDKLSDDFRTILEQSVAHVFPSCSEAGAAAVIETMAAGVIPVVTYESSIDV
                     ENFGVLLQDASIETIIRQVREIAAMPVEELKRRAQKAWEAAHCNHTPEKFERAYRETM
                     EMILAKHGKR"
BASE COUNT         3892 a         4514 c         3779 g         3662 t
ORIGIN      
        1 tcgggaacac ctcaagaatc tttgcaaaaa accctttggc atagacggga gttcccaaat
       61 cgcgcataca tcgggagaac acggcataaa attcatccag gcactcgttc ccgcctatgc
      121 gtgctgtcat cccttctttc tgagctcggc gaacctgact gcgtaacttt gaagggaacg
      181 cttttaccag cgactcgtat gacccaggca atgacagcct catcgaaact tttcgttcgg
      241 tggaaaccca gccagtctct atcggccgtt catgtcgaag ttcgatatgc gctgcatcca
      301 gcgcgttcgc ctgttcaatg gcaccgcctt ccagcactcg atacgcatcg ggagaggtag
      361 cgacaaggcc tccgtagttt acgaacggca gagaaaccag aaaccgacca aatacccggc
      421 tggccaacag caccaacggc acgatgccct gcacagctcc gtgttcgtcc cgagcgatca
      481 gataataggt acggtggcca aaggcctcct ctatgacacg gcgccagcct gccagatggt
      541 atccggatgt aatgggagag ttcagaacgt acgtatccca tgccagaaca tccgagtcgg
      601 ttcgcccgag aactgatgag accaacgtat tcatacagcg attaccctac cggtctggga
      661 cgaacggcgg catcgagaat agccgctaat tctccggtca gcgatcgata ttcaaaccga
      721 tttacccgag cggcatcggg cggaacgagt ctttgcgttc ccgttgtgag gtctttcaat
      781 aacgccttga tgcgtgcaat gtgattcggg ctgcttacgc ctaatccatg ccgagccacc
      841 aaattggcag ttgcgccttc cccgccaata agcaacagcg gccgaccggt cgcaacatat
      901 tcgtatatct ttgcgggcac ctgcaaagga gagtctggct gcaggacaag taagacgctt
      961 gatcgttgca tttctctgag gcacacgcta tgcgaaaccg gaggttcgcg ccggagaaat
     1021 ccatatttct ctagattggc ggcataatac tcacactctt ggtctgttgt gtcccagccc
     1081 ccgacgaaac gcaatcgaat ggcttccggc tttaactggt tctcctgaaa caactcccac
     1141 acggcctgca acaaaatccg cggggttctt tttccataca cggttccgaa atggcacaac
     1201 tcatatcctg aagacgtgag ccccaggccc gatcgctcct gaggcacgtg tgggggtgac
     1261 agcgtctctc gatcgaatcc attggggatc cacgtgcagc ggtctcggag aaattcgtat
     1321 tcttcaataa gtcgttcgcg aagctcgtcc gtattggcga tcacatgact tgcaccctga
     1381 cagacttggg cttcagttgc tcgtgatttc ctggtcaacg catccgagtt gaaggagtaa
     1441 taaggattac aattccaggg gtcccgataa tccaacacca gcggacaatg aaagtgcttg
     1501 gcgagcgtgt cgccgatcaa aaaatttgtc catggcccgc cggtcgcaaa aatcacatgc
     1561 ggaacatcct tctcgtgaag agtctgtagt ttccgcacag ctcggctcag ccacccgcat
     1621 tgacgatctg gaaatgcgaa cgcccaatcc agaataaagt ctttcagcgt cgaccggcct
     1681 ggactcgaat caggtggtct caccgcagca ccggggttgc tcgcggattt gtgagcaggc
     1741 cctcttcgta ccatcccctt cagcatctca cggtactgca agacccgctg aaggcgatct
     1801 atatacggca ctcgaatgac ctcaacatca gcaggaactc gctcgcctag cttaagatca
     1861 acctgatgcg acggatatac acactccgga gtggtcgtga gcacctgagg ttgccagcca
     1921 taggccgcca ggtttcgaca aaatccaagc ggtcgcatgg ccccactcgc cgcaacgggg
     1981 ggaaagtaat aggccaccat gagtactcgt ttcaccgtct caccgccagc tttatgcaaa
     2041 atcatgattg tcggagatta gtccagtaat ctgatgaaat gaatgtcgtc acagaacaac
     2101 tgaccgggaa tacgccctca ggtgctgcgc acttcaaacc gttctagaat ctaggctggc
     2161 gactgccctg caagatctga caatatttca aattcggcga catggaagag tctattcgaa
     2221 aaatctgctt ctcctcggcg atgccgatcc aagtcagcta tgagagcacc cgtgttatag
     2281 attcctcgct ctcgcaaggt gcgactcgcc aggatttctc gaagcggctc atacaagtca
     2341 tgcgcgaacc actttttact cgcggtagga aatcccatct tgtccactct gctccgcaca
     2401 gactcaggga ttctgccctt catcccctct cgcagcacat atttgttcca cggacctcgt
     2461 accttccatt cgtcagatag accgcacaca aacgacacga ggcgatagtc caagaacgga
     2521 agtcgcgcct ccacagaatg agccatggag tttcgatctt cgatccgaag atataagggt
     2581 aacggagccg atatcaccga ctgttttaat gctgtggaca aagcggcgga actgggcggg
     2641 gtatcttcgc tgataaaatg tttcgttaaa tcaccagaga accatggatt ttgacgaata
     2701 cgggcttgcc ttctagcctg agcccatgtg cggtagctgc caatcttgta catctcccag
     2761 gaaatgcaac gacttgccgc cgctgtcata cgcgcacgcg aatttcctcc atgtgcctcg
     2821 gtataggctt tcaccgcctg ccaggcagca ccgacacgcc cttgttgcag gagagacacc
     2881 caatagtctt ggaaatagct tgaatacccc ccaatcgttt catcggcgcc ctgtccattc
     2941 aggacaacgc gaatgccttg ggaggcggcc agacgcatga gttgatagcc gaccacggcc
     3001 gtcatggtat gcacaggctc gtcctgaaac cagaggactt tgcgcacatc attccagagt
     3061 tcggcggggc ttgtttccag ctgtctgagt tgagcgtgag tttgaagcaa cgtatctgca
     3121 atatactttg attcatcaaa ctctttggcc atatagcaga acgcctggag agactccggc
     3181 tgcgtccccg cactttcgtc acggtgacgt gcagcagcac aaatgatcgc tgttgaatcg
     3241 agcccaccgg aaagacacac gcccactggc acatcacttc gcatacggat acggactgag
     3301 tcttcaaaaa ggtcaccgaa agtcgccgcg ggatcaggag cgatggtttt cggcaaggca
     3361 tcaagtgtcc aaaacgtcca ttgatgccac gcaccatcta atccgacttc aaacccgcta
     3421 cctggaggaa tctgctcaat cccgtcgtag aatgtctcgt tatgactgtc cagacggcct
     3481 tccaatagaa atctggacgc aattcgccaa ttgatcccta cccgttgata gcccgatgca
     3541 cgcagtgcct taatctctga ggcgaattgt atcaccgtgt gatcacggct gtagtacaac
     3601 ggtttgacac caaatcgatc acgtgacccg aaaagccgtc gatgtcggcg atcataaatg
     3661 acgaacgccc acatgccgtt tagcttcggg aggcactctc gcccccactc ccggtatgcg
     3721 gcgagcagta cttcgctatc tccgctcgat cgaaactgat gccccagctt gcggagttca
     3781 tcccggagtt cgacatagtt gaagatttcg ccattgaaca ccaggacata ctgcccatcc
     3841 tggctgacca ttggctgatg gccggcttcc gacaaatcaa gaatggagag ccgacgaaat
     3901 cccattccaa cggctccatc catatagatg ccgccgtcat cgggacctcg atgaagcaag
     3961 ctcttggcca tccgctccac cgccgcccgc tccaccggcc gtccattcag ccctatggca
     4021 actgcgatcc cacacattga agtagcccgt ctccttatcg cttttcgcgg attggttgtg
     4081 gcgcgtgaat cacttcacca caacctgacg aaaatactcc gtcgcttttc tgataccgtc
     4141 ctcgagctcc actttgggct cccaaccgag caggctcttc gctctggtga tgtccggacg
     4201 ccgaacttta ggatcgtcgg ctggaagagg tcgctcttct atgacgctct ttgaaccggt
     4261 caacttgagg atgatttcgg caatgccccg aaccgtgagc tcgcgagggt tcccgatatt
     4321 caccgggtcg tgaatactat cgaaaatggc gtcgggcttc tgcgtgagaa atttcgtgcg
     4381 atcggtgcgt tctgcaagag acttgtcaga tttcaccatc agtagtgcca cgataccacg
     4441 aaccaaatca tcgacatagc aaaagctacg tgtctgagag ccgtcaccaa acaccgtcaa
     4501 tggctttccc tgcagcgcct gcacgataaa gtttgacacg acacgaccat ctttgggccg
     4561 cattcttggc ccgtaggtgt taaatattcg tacaatccgc gtgtccaccc catggtatcg
     4621 gtggtacgcc atcgtcatcg cctcggcaaa tcgtttggct tcgtcgtaca cgccgcgcgc
     4681 gccgatggga ttcacatttc cccagtaggt ttctggctgg ggattcagca acggatcacc
     4741 gtacacctcg gaggtactgg ccaatagaaa gcgagcgcct ttggccttcg ctaaccctag
     4801 cgccttgtgt gtgccgaggg cgcccacttt caaggtagca atcggcatct cgaggtaatc
     4861 ttgcggactg gcaggggatg cgaagtgcat gacggcatcc aattggccat cgacatgtaa
     4921 atagtcgcaa acgttgtatt ttacaaagct gaactttgga ttgccgatca agtgcgcaat
     4981 attctctgcg cgccccgtga tcaaattatc aagcgcaatg acgtcatggc cctgtccgat
     5041 aagaagttcc gacaggtgac tacccagaaa ccctgcgccg cccgtgatca aaattctcat
     5101 gaatcgctcc ctccttcgtg tgccctaatc taggctaatc gagttcaaac tctttcttcc
     5161 aatctgaatc accctcgagc ggtttttgat ccgctttcag tagcacgcaa ttctcgagga
     5221 ccaatacatc catttccgtc cgcatgaagc accggtatgc atcctccgga gtgctgacaa
     5281 tgggctcgcc tcgcacgttg aaagacgtgt tcaccagcac cccatgtccc gtctttgcct
     5341 caaacgcttt cagcagcgcg tagtaacggg ggttggtgtc ttgatgaacc gtctggaccc
     5401 tcgccgaata gtccaggtgg gtgacggcag ggatatcaga tcggggaata tttaagagct
     5461 caatgcccca cagtccattt tgagctgaac tgaccggaag gcgtcgtttt tcgaccacgg
     5521 gagccaccag caacatatat gggctgtccg aatcgaggac gaaatagtcg gatacgcgtt
     5581 cgcgcaacac tgacggggca aatggacgga acgattcgcg atacttaatc ttcaaattca
     5641 tcaccgactg catatctcta ttgcgggcat ctccaagaat gctgcggccc cccaaagcac
     5701 gaggaccgaa ttccatccgc ccttgcaacc agccgacaac cttgcctgcg gctagctcct
     5761 ccgccacccg cgcgtacaaa tgatcttcat ccagcctgac atagggcgca gcggcttttt
     5821 gtagatagga ttctatttcc tcattggtgt gtgaaggccc taaatacgac cctttgatgc
     5881 tatcttttcc cggaacgact ttgcgaggct gctgctcata ctggtaccag gtcgtcaacg
     5941 cagcgcccaa tgcgcctccg gcatcacctg ccgctggttg tatccagagt cccttgaacg
     6001 ggccttcccg aagaatgcgt ccattaccga cacagttcag cgcaactcca ccagccatgc
     6061 acataaagtc agcaccggtc tcgcgatgta atgtacggga gagccgcaac atcacttctt
     6121 ccgtcacttc ctgcactgag cgcgccagat ccatctcgcg ctgagtcagt ttggattctg
     6181 gctttcgggg agggcctcca aagactttat cgaacttgtt cccggtcatc gtgaggccgg
     6241 tacaataatt aaaatagtcc atattcagac gaaacgtccc gtctggtttc acatccaata
     6301 aatgctcgta aatggccttc acatacttgg gttccccata tggagcaagg cccatgacct
     6361 tatactcacc tgagttcacc ttaaaacccg tgtagtacgt gaaggcggaa tagaggaggc
     6421 cgatagaatg cgggaatgga atttcccaaa gcggcttcag tgaattgccc tccccgagcc
     6481 aagctgatgt ggtcgcccat tctcccaccc cgtccataca aagcacgaca gctttgtcat
     6541 agggcgatgg aaaaaaggcc gatgcagcgt gcgactcatg gtgttcagag aataggatct
     6601 ggggcaagga tgcggtcgtc aactcgggcg cgtgaacgag gaactccttt tggagtagac
     6661 tcttgagtag caacttctct tttagccaca ccggcatggc agccacgaaa gactgaaccc
     6721 ctcgaggagc aaatgccagg taggtttcta acaaacgctc aaacttcacc agtggcttat
     6781 cgtaaaagac aaggtgccga acatccttga ggcttatatt cccagcctga agacagtatt
     6841 caaccgcctt atgcggaaac cctggatcgt gcttctttct cgtaaagcgc tcttcctgag
     6901 ccgctgcgat gatttctcca tcacgtacta agcatgctgc actatcgtga tagaacgccg
     6961 aaataccaag gatgttcatg cctgcccccg ccggtagaag gaaagattgc actcacaacc
     7021 agtctagcat ttgattgcac ccgcaacgaa ccataaacag aaaaaagctc aatatttcta
     7081 agagataggc aagaaactag gactatgagg cactctgaca ttcgtgaata cagctaaggt
     7141 atcgcgaggc gatgccttcc cagttgtact cacgacacgc ccattctttt ccccgcatag
     7201 caagcgtttc tcgaagcaca ggatccgtgc acaaccgaat aagaccggat gctaacgcct
     7261 caggattgtc gggaggtacc aagataccgg ttgtaccgtc gtggacgatt tcaggcacac
     7321 catctacgtt gctcgcgatc acagctttac cgcaggccat ggcttctgca atgaccagcg
     7381 gcattccttc cgcgcgcgaa gccaatacaa aagcctgaca ccatttcatg agccggagca
     7441 cctgctcgtg agtttgattc cccagaaata tgacttggtc agctaggccc agttcgcttg
     7501 actgacgtag caagttttcc cgttcaggcc catcgccagc cacgacaaga tgaaggcaca
     7561 cccctcgctg cttggctttt gccaatgaag caatgacaat atcgatccct tttctgtgaa
     7621 tcagatgccc ggcggtcagc agatactgtt gtgggaggga tgtgtctgcg gatccaccgt
     7681 tgacagcctc aatgacgtct aagggcgcac catttggaat cagttgcctt cgcttcagct
     7741 gaattccttg gaacgccgat tccaccttag acaacagcgt tcgcgaaacg cccagtacca
     7801 tatcggcttt ctccagcagg gaccggacca gtcgttgttc ttgcggactc catgacgaga
     7861 gatcgtgtgc atcgttcccc tgataggtca cgaacaaaat tcccgtccac aatcgcttca
     7921 agatcccaaa ataatacatc gcaggcaggg gatactgaat cagaatggca tctaattgag
     7981 acctgcgcgc caaccataag agttgcatca ctgtcagcgg aaaccatacg taatacatga
     8041 tggccgcccg aatcggatga tcggacgaca caggcgaacg cagatacatg ccatagaccg
     8101 gggtgtcctt cagtacttcg atttgagaaa gacgattctc gcggtcagcg acaaagatcg
     8161 ttacgcggtg cccttttttt ctgaattcct cggacaggcg agctactacc gtactgattc
     8221 cgccggtgag agatgggcgc cagggagtga ctaagagaat gttcatagcg tacgggatat
     8281 attgagctgc aattttattg tcaggcaatg gcggctacat caagacggac tcaccggcat
     8341 ttgaactttg ggattcgaga gataggactt ggccagatcc agatagagtt cgagatgaaa
     8401 cggccattgt cgaaccatgc gccgaaggcg acgacgggct actgcacctg aacattccag
     8461 tcgttcgaca taagccagct gtcgctgatt tgcgtacagt tgcgtatcaa taagttgccg
     8521 tatctcattt ccgtgaactt ccccaatccg gtgttcaccg agaaggatct cataaatctt
     8581 cacaaaatcc cccagcaatc ggcgttgact ggacatgaga ctccctgccc tccatcgata
     8641 ttgacctaag ggctcaggca aatacccaat ctcatatccc tgcagaacaa cctgaatcca
     8701 atgcaaataa tcctgggcaa ccggcagcct tagatcaaac ttccccgcac gttcaaatgc
     8761 acttcgccgc tcgagtaccg tcaaggtttg gatatgggag cgctgcagta tgtctcgtag
     8821 acacagtcct tgcggaaccg tcctcttggt ttccttgtta aagcgggcat ggaggacgcg
     8881 atcttcttca tcgatcactg ccacttcggt gtgaacaaaa ccgcaggagg gattggcatc
     8941 gagatacgcg acttgacgcg ccagcttctg aggctcccat aaatcatccg cgtcgagata
     9001 tgcgatgtac tcgcctgtcg tagtgttgag ggctgcattt cgtgctgcag aagctccctg
     9061 gttcgcctgc cgtatgtaat ggagcgcgtc tccatactgt tccagcaccg cctgggtgcc
     9121 atcggtcgac ccatcatcga cgacaatgat ttcaaaatcc cgatacgtct gccttagcgc
     9181 cgaatcgaca gtgcgtccga tgaaccgttc gcaattgaat gtcgggatga cgactgatac
     9241 acgaggcatc ggcaagctta cccaggaaat gacctacgtc accttggccg acagccgggg
     9301 tcgcaacgct gtcagagtct tctccgtcgc tgtttcccat gaaaaccagt cggccgcgag
     9361 tttcaaggca ccagctcgaa ggcgatcacg caagaccgcg tcgtccatca cgttcaacaa
     9421 caaccgggcc acggcttgat gatcctttgg ttcacaggtc agaacatttt ctccatggat
     9481 aaacgcctga tccatcagtg gaccacgtgt cacaatgatg ggcagaccat gtgccaccat
     9541 cgatgcgaag gaactattgt ttaattgcac accttccagg aagggcagga cacacacatc
     9601 cgaagcatgg agtaacagcg aggcatcttc ttcattggac ttaaatgctc ccgtccaggt
     9661 tgtccgtccg tctatctgta acgtcttcgc aagggcctgc atctcatcaa aatatgacgc
     9721 ccctccttga acatcgaggt ctacttttcc tccgataaac agaagcctgg cctcaggtcg
     9781 ttgcgcggaa acggtcgcaa atgctcgcaa gagcgtttca attcctttaa tcggatagag
     9841 atatccgaaa aatgtcacca caaattccgt aggtccgaca cctaatcgtt ttcgacctcg
     9901 ctcacgggcc tcacctccat cgcttgaggc aataaataga ttcggcggcg gagggatcag
     9961 ggcaacttta cctcctacag gaggccactc ttcgatcaac atggcacgat gacgttcgca
    10021 cagcgcgatg acggaatcac tgtctcgcag caaggttcct gaactataga cgacatcacg
    10081 gacacccgcc cagcgcacca taagcttccg aatcactcgt gataccaatc ctgtctttga
    10141 tggatcggct ccgacaaatg cactctcata gcgtgtaaca aacggcacgt tcggaaagag
    10201 ctttttgcaa agggtgggaa ggaacgtcac catcgggtga aacttataca tcagtccgat
    10261 gtacatcaga aacactgcat ccggcttaca gctcttaaga aaggttcgga cgcgtggcca
    10321 ctcagtccag ccccagccct gcatgatcgg atgcacatga attcgcggat cagcggttcc
    10381 aacatttccg actgaggtga gaacatgcac ctcaattcca cgctcaacca attggttgca
    10441 gaggtggtag gcattcgtag cctcccctgc atgcatcggc ggatacgcgc ctgaaatgag
    10501 caagactttc atggcagatg aagcgtctct cgttgtcatc gctgtggcaa gactagtgac
    10561 tgacattcat cgtcactgtt cgccgcaccc acgactggtg aatctcctca aagatgtgcg
    10621 acaaggtttt tgtaattccc caggtaggat aatgcgaagt cattttcttt agattgctga
    10681 tatagcaaat gtgatcgcct tcccgatttt tttcgacata ctcgtacaac atctttttcc
    10741 ccgacaccaa ttcaatcatc tcgaacgcct ctagaatcga gcaactattt ccgcgtccgc
    10801 cgcctagatt gtacacttcg ccgcttcgtg gcttctcgat gaatgcgtga atgaagcgcg
    10861 cgacatcata cgaatggata ttgtcccgaa cctgtttccc cttatagccg aagacactgt
    10921 acttcttccc ctccaggttg catttgacga gatagctgag aaatccatgc agctctaccc
    10981 cagagtgatt gggtcccgta agacagccgc ctcgcagaca gcaggttttc attccaaaat
    11041 aacggccata ttcctggacc ataatatctg ccgcaacctt tgaggcgccg aaaagcgaat
    11101 gctttgattg atcaatacgc atgtgttcgg cgattccgtc aaaatcattg ggagatgcgt
    11161 agtcccaccg tttcgcctgc tcgataagag ccaattcgtt cggagcatcc ccatacacct
    11221 tattcgtcga catatgcacg aatactgttt caggtgtatg caaccttgtc gcttcgagta
    11281 aattgagcgt acccacggca ttcgtatcga aatcatcgaa cggaatcttc gcagccaaat
    11341 catgagaagg ttgcgccgct gtatgcacaa tgacatcagg cttgagttcc tgaatgagcg
    11401 ctaaagcccc ttgacggttg cgaatgtcca actcgtgatg tcgaaaattc tttaaatcag
    11461 cctgcaatcg gtgctgattc cagcgcgtat caccttgcgg gccaaagaac gtcgctcgag
    11521 tgttattatc tacgccgtgg atcatccagc cttgaccatg aaaataggcg catacctccg
    11581 acccaatgag gccagatgaa cccgtgacga gcatttgttt catgatgtac tcactccctc
    11641 ctgttctcaa ccgctaccgg agcagtccct ggcccagatt cctgctactg ttccgtactt
    11701 tcacaagccc actgatcctg gtgtcatgta cggggccaga agcgcgcgca ccagcattct
    11761 gaaatccaac gctcttttat gacgtctcaa caatacgcag ccttcaagcc tattgtctgc
    11821 aattcacgca atctgctcaa gctcattagc attctcgaca tgcccttggt ccgtctaaga
    11881 aaccagcagg tggggctccc agaatccccg gtcatatcgg ggccaggcgt gacaaccgaa
    11941 gggcagccgg cggtgattca tgtcataaca cagccgtggc gccacttcga atgcaaagcg
    12001 cagtccgatt tctaccgagg caactttaaa atccggcaca taatgcgttg cccgatcgga
    12061 ccaaaaatga tcttcgtttt ttgtcccgtc aggcctgaga tgccagcggc tcatttccag
    12121 ccgagcatta ttgaataacg atagacgttt gataagacgt ttgggggaat ttatccatct
    12181 gaggtaagtc ggcatccccg catatttgtc ccgccagtat tcttccgggt ccatccaata
    12241 ctcatctgag tgaaacacct tcagaaaact ctgaattttt cgcagtgaca accccccatt
    12301 tccaactcgc ggctccttca cccaagggct atcgacgcac ggaatccacg gtggaccgat
    12361 gtagtctaat cctgtatcac accaggtagt gagttgatcg gaaaaaacca gcgcgtcgag
    12421 atgatagatc aaaatatatt catactcact gaacgcacga taaaaatgct ccgacagcag
    12481 gaggcgtgta ttcgcaatcg cgctgccgaa atactcctct ccaaatcgct tcagattgca
    12541 accaggcaag gtcacctgaa gactttccgg caccacaagg tacttgtcat agggagcaag
    12601 gtagtgcgtg agatgcctga aagaaatttg ttcgtcgacc gtcagggtgc ttcgattatg
    12661 catcggaacc accacggcta cttttttctg cgacacaggc accctttcat ttggttcaca
    12721 tagtatctgg ccgaagggct ttcgcgaggt caaaaggaat gtccaccgct acctacaaat
    12781 atcctgtccc ttggggacca atgcccgcaa cttctacaat ttcgccaact gccggatgac
    12841 ttcttcgccc accaacttgt ggccggcctc gttataatgg gcatagcgtc ggaatggaaa
    12901 aagtgccaag gggtccggtt gcgcgttaaa cacaggatgc atatcgatta ctggcatctg
    12961 aagacgctcg acgatcttga gcaccttatc acgatcctgg ctagccgtct caggtatgcg
    13021 ataccgttcc cacgtcggca aataaacaaa taccacccgt ccgttccacg cactcgccgt
    13081 agcctgagct tcagcgagaa tagtcccaaa tagctgaaga tcttccggcg cagccggtgc
    13141 acctgtatga cgcaggtatt ccagcaattc ggccggcaac ccttgttcgg tcggccgccg
    13201 ctcatagaac aattgcacag cctgacgaag gtgttgcagt ttcacaacct tttcaaaaga
    13261 aacgggcgtt gcagctttcg ccatggcagt atcaaggaac gctctcaatt tctggtcaat
    13321 ttcggcttgg cgcgagaaca gccgctgtga gaacgaagac gtcagatatt tccgcaataa
    13381 tggcgaattt ttctcccatc catcgaggtc ccgcaaatca ttgccttcat aataaaacca
    13441 caagacatgt ttcggtttga gaatcggtcc gtattctttt aatgtggcca aactcgtcat
    13501 cggcccatga ccattgacgc caagatttat cgtaccggaa tgccgcgatc gaacgacggc
    13561 cacaaaccct ttatcggagg atacacagac tccatgcgtg tacgaatctc cgagcgcaac
    13621 aatttcggct gactggttcg cccagatacc tcgtggatta tgaaatccgt gttcgtcgct
    13681 ttcatacacc acgtattcgc cgctttcatt gcaaaatact gttgtagtcg ttgccatccc
    13741 cgcgactggc aaaaactctt ccccattgat ggtcagcgat gactgaatgg agacgccaga
    13801 tcccggctca aaaagaacca tcgggaacac atcggggaat gcattgatac cccgtgcgcg
    13861 catatcttga atcacttcca atctcgaacg agtgtcgaaa tgttgctggg tctcctgaac
    13921 cgttttcatc cgctcagctg caactcgggg atttgcatcc tgaggaaaac tcaaccatgc
    13981 ctgtgcgccc aacgcggcga tagctgaccc ggaatatgca aggagcaatt cgaccccgta
    14041 taccgcaatc gcaagtgaca tcccgcaaag cacactattg atcttgtagg tgtccggcat
    14101 tcgaagtccg agcgcgaaga caagggcaag cagaaggaac accgcgtaac gtatagacat
    14161 gccgtgacgc cataccatga cagcgaacag cagcagacat acggctactc caaacagcag
    14221 caccctactt gcgaatttcc ccaagcggtg cccgatgcta ctcattcgat gccttctcag
    14281 ttctattcac gaaagggctt ctctatgaat ctgaacggta attcagccat tcgaacaaca
    14341 ccacaacaca gactctcaac ggttgttcct accgtttgcc gtgcttcgcc agtatcattt
    14401 ccatagtctc acgataggca cgctcaaact tttccggggt gtgattgcaa tgcgctgctt
    14461 cccaagcttt ttgagctctt cgctttagct cctctaccgg catcgcagcg atttccctca
    14521 cctggcgaat aatggtttca atcgaggcat cctgcaagag aactccgaag ttttcgacat
    14581 ctatggaaga ttcataggtc accaccggaa tgaccccggc agccattgtc tctatcaccg
    14641 ctgcggctcc ggcttctgag catgatggaa atacgtgggc cactgactgt tccagtatag
    14701 tccgaaaatc atcgctaagc ttatcgagcc atcccacaca cttaatattc gatgtatgaa
    14761 acagttcttt tcgatagacg ttcacaaact ccggttcgtc atgcaccggc ccgacgatag
    14821 taagctggca ctcggggact tgggcgaagg cttctaacac cacatcgagt cccttatgaa
    14881 ccatccctcg actgccgaac cataggaatc gatgccgcga agcctcaaag tcttttgttt
    14941 caggccaagg ccacattttt tgagcgagca tgggcagccg gaaaattggt tttccggaat
    15001 acgcaaaggt attcatcgtg aactcattgc cgcacgtcgt gagatagtca gcctgttcaa
    15061 cacctaagtg cggggtttcc agacgattca agggaagagt cacgcctctc cgtgcctgaa
    15121 atgccaatac acgagtcatc tcggccacgt tctgatagac cagctgcgcc gtgtcacagt
    15181 gcagtatctt gatacaattc gacggcagat agggagcaag cctctgtaag ttaaagcgag
    15241 tatcgacgac tacatcatat gacttccagg gaacgaattt gtgattctcg cagtgaatca
    15301 cgtcgacgtc gtagccgatc tctacaaagg tctgagccat gaccatggtt ttgtaatact
    15361 gcgggtggct ggtcggaatc ggctgtcccc gcatcttgct taacaatccc ccattatcgt
    15421 aggagaccag tacattcccg tgcggattcc cggcggcatg caacgataca accccaccgt
    15481 gaaactggcg tcggactccg cagagagcag gtacgagttc gcgcatccag agataccact
    15541 gataccgttg caaatatcgt cgcatctgtt ttaacacgac gctcgttccc acccttcagg
    15601 gactaaccag ccgactggcc gccacccatc caccgccgca atccaccgat tttcgtccag
    15661 acgatgagga tcgatatggt gcattcctcc ttgtgtccag tgtctccacc ccgcaccgaa
    15721 taatggactc ttcgacaaag gacattctcg gtaggttgtt gttgtcagat ctgtgatttc
    15781 aaatcctcga accgagcccc catagacagg aaaacaatcc tgcgcaaacc gcatcagtcg
    15841 accggaa
//
LOCUS       DAOQEW010000080        15846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1003_length_15846_cov_9.050725, whole genome shotgun sequence.
ACCESSION   DAOQEW010000080 DAOQEW010000000
VERSION     DAOQEW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15846
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1003_length_15846_cov_9.050725"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="fabF"
                     /locus_tag="PLS35_10135"
     CDS             <1..1061
                     /gene="fabF"
                     /locus_tag="PLS35_10135"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNI19067.1"
                     /translation="FIEKKEIKKMDTFIHYAVGASQLAVDDAGLKVSAEEATRVGVYI
                     GSGIGGLGSIEHYHDVLKEKGPGRVSPFFIPMTIINLASGQVAIRVGAKGPNSCAVTA
                     CATGNHCIGDAYRLIKRDDADVMIAGGAEAAITPLGVAGFASAKALSFRNTEPAKASR
                     PFDKDRDGFVLGEGAGVVVLEELEHARARGARIYAEVIGYAMNSDAYHITAPPEEGEG
                     AVRCMEMAIKDAGVAKTDIGYINAHGTSTMADAIETKAIKHVFGEQAYKIPVSSTKSM
                     TGHLLGAAGGIEAVFSILALHHGILPPTINLDHPDPACDLDYVPNKARATKTQVVLSN
                     SFGFGGVNACLLFRRCDQ"
     gene            1089..1796
                     /gene="rnc"
                     /locus_tag="PLS35_10140"
     CDS             1089..1796
                     /gene="rnc"
                     /locus_tag="PLS35_10140"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNI19068.1"
                     /translation="MTSATSIEAVQRLLGYRFRQPRLLEEALTHKSYSNERRTKDRTQ
                     NERLEFLGDAVLSLVMSEYLAAEFPGSNEGGLSKLKAHLVSEASLAKAARRMKLGRLL
                     RLGKGEELSKGREKHSLLADALEALIAAVYLDGGLEASRTFTLRVLEEELLAARAERA
                     RPGMDDYKTQLQELCQKRFESLPQYATVRESGPDHEKVFEVQLTIQGVMRGVGCGHSK
                     KEAEQMAAREALTQLTT"
     gene            1826..2479
                     /locus_tag="PLS35_10145"
     CDS             1826..2479
                     /locus_tag="PLS35_10145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNI19069.1"
                     /translation="MMTQSRWHNRFGCAAVMSLVLAIGMATTGLAADAPAKAGDSVNA
                     SNVRAVIKTKVGDIEIKFLPDVAPKHVENFIKLAKSGFYNGTIFHRVIPGFMIQGGDP
                     NTKDSLRKDAYGQGGPGHNVKAEFSDLPHKRGVVSMARAQDPDSAGSQFFIVVEESRF
                     LDRKYTIFGEVVKGIGVADKIVALPRVMCQTGAPSPADKGPCDNPIDRVEMTVTIIE"
     gene            complement(2545..3120)
                     /locus_tag="PLS35_10150"
     CDS             complement(2545..3120)
                     /locus_tag="PLS35_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19070.1"
                     /translation="MQKRQANLWGWWLRRVGLTVACFCVVLLPTTGFGQSIEDTVQFI
                     SDMANTHGFVRSLSCRNPKAAKPTKVTEIYSVIPTGTRLGTIELNHGHDDVNTGFTHF
                     NLQDIESIAYQGQDTRENNLVLYAVRISCNSNGPCILKTSFCTGAVTEVKGQFPEDTL
                     LFRSASHAERMAKALSHFLTLVRTQQQNQPF"
     gene            complement(3283..4173)
                     /locus_tag="PLS35_10155"
     CDS             complement(3283..4173)
                     /locus_tag="PLS35_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HNI19071.1"
                     /translation="MHCLTFDIEEHFQVSRFDSPIRRRHWGAFESRVTANTCKVLDLL
                     ARYDTKATFFVLGWVAERHPGLIKQIAECGHEIASHGYGHELVTAQTPELFRADVRQA
                     KQILEDLIGSPVQGYRAPGFTITRETLWALPILAEEGYTYDSSIVPIRHDHCGLPGSD
                     PWQHLKQTSSGPIWEVPPSTVNLGGVRIPIAGGSYFRLLPFPALCGLMRRIEQKGRPL
                     VMYFHPWELDPYQPHMEGPFLSKMFHYLNLDKMEFRMSTVMNSFRFGSIAEQIDLSPI
                     LPIDPTVFPSTPAEIAQDVV"
     gene            complement(4475..4951)
                     /locus_tag="PLS35_10160"
     CDS             complement(4475..4951)
                     /locus_tag="PLS35_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19072.1"
                     /translation="MADDVPAALCGDPTRLQQILSNLLANAIKFSDRGTVSLTVDLDQ
                     RPSEQRLEFPATSYGELSSTASRISHVRFSVADNGIGIAPNAFAKLFQPFVQADGSTT
                     RKYGGTGLGLAICKQLVELMGGHIGAESEPGAGSVFRFTVPLQQPLADNERNKSAA"
     gene            complement(4973..5722)
                     /locus_tag="PLS35_10165"
     CDS             complement(4973..5722)
                     /locus_tag="PLS35_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19073.1"
                     /translation="MNPLTSGRSPYALVTDDDIIIRMFAREALEQAGWEVEEAENGLE
                     ACAAFKKCPPDVVLLDVMMPEMDGFQTCAALRQLPGGEHTPILIMTGLDDFESITKAY
                     DAGATDFIVKPLNAMLLTHRIRYMVRANQVLQELRASQATLTQARDAAMEGARLKSEF
                     LATMSHEIRTPMNGVLGMTDWLLETELTAEQLDCAQTIRSSGDSLMVIINDILDFSKI
                     ESGKLSLELLDLDLPKFVDRVLALFAERAQR"
     gene            complement(5719..9342)
                     /locus_tag="PLS35_10170"
     CDS             complement(5719..9342)
                     /locus_tag="PLS35_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI19074.1"
                     /translation="MHPLLTRQLKRLGLETTAAPTSLEAWQQLLERVSRSYIESDQGR
                     ELLERSIALSASEMQELNEQLRRTSESQLTQERDKLHTVLRSMGDGLCVVDRNWDIVL
                     LNPEGQRLWGLPEADVVGRRLPDMISLSYGTNLTEPIFTQMLLDETAQGHSFRTDDGI
                     LTSITGRSFPVSCVLAPIVRDGQAGGAVLVFRDISERKQAEDVRRHTESLLRQHQAAL
                     FGLTSNSIIQSGVLEPALREITRVTAATLGVRRCSIWLLRDDHSAIICKDLHDASNNS
                     HSCGMELLAAQFPKYFRELLTERVIDASDARTDPRTSEFTDTYLTPLGISSLLDIPIR
                     FKGKLVGVLCNEHVGTPRSWMLEEQQFSYAVASLVSLALEAVERLHAEGALRKSEGRT
                     RLIIDTALSSVISIDEQGRIIGWNTQAEQTFGWTRQEAIGRLMAETIIPASQREAHQR
                     GIRQFLKTGEGPILNKRIEISALRRDGTEFPIELAITPLRLENAYTFTAFVVDISERK
                     QAEEALRTSEARLTMTVQGSHIGIWDWNLTTDSVYFSPQWKSQLGHDDTTLPNAFQEW
                     QSRIHPEDRALVHQTVHSCLNGDRAQFELEHRLRHRDGSYRWILSRGTLIRDVYGVAA
                     RMVGIHIDTTDRKRTEEELRAAKEAAEAASKAKSEFLANMSHEIRTPMNGVLGTTELL
                     LNSTLTDKQRHLASTVHRSGRTLLAIINDILDFSKIEAGKLDLECVGFDLSQLLDESL
                     ELFMEAARRKHLHLTQQVDERVPRYLMGDPVRLRQILMNLLSNAMKFTETGGVSLTAD
                     YMSGTPTHAQLRISVTDTGIGIPPAAKLRIFDAFAQADGSTTRRYGGTGLGLSIAKQL
                     VGLMGGAITVESEPGRGSTFSFTARFELQPLGTGTDNGLPSYERLAKPHLTLEPPYGP
                     SEPYPSSPGTEAKRTGHRVARILIAEDSPVNREVAVGMLEILGYQVEMAENGRQALEL
                     AGRSQFDLVLMDCQMPEMDGFTATGEIRRREAASGQRRLTIIALTANAMQGDREQCLA
                     AGMDDYLTKPYTQVQLQTVVQKWLDKQGTDRALAPVFSSTQALSGESAPAAAAPAPST
                     PSMDPKALDGIRALQRPNRPDVLASVLRKYLDNSRDSVDALQDAIRANDPAGLQAVAH
                     RLKSSSAQLGALAVAARCKELEALGAQKNLIEADRILVELQSEYAAACAVFRNEIAKE
                     KSS"
     gene            complement(9348..10496)
                     /locus_tag="PLS35_10175"
     CDS             complement(9348..10496)
                     /locus_tag="PLS35_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNI19075.1"
                     /translation="MHVATTSLKAGHADIPAELAAHDSENSLLLLFGSSSLIDRPELI
                     QQVMAACPRSHVMGCSTAGEIHGVEISDDSLVVAAVRFEKTRLLTTQAAVHTPKDSYA
                     AGRAIAASLHQPSLRGVLVLSDGLNVNGSELVKGLNDTLGGSVVVTGGLAGDGTDFKR
                     TWVIKDRAPQSGYVTAIGFYGDHVRLGHGSKGGWDKFGPERRVTKSAGNILYELDGRP
                     ALQLYKEYLGDRAAGLPATGLLFPLAIRSSNTDGKVLVRTILAVDEAAQSMTFAGDLP
                     EGMLAQLMRANFDRLIQGASEAATLTLNNHEPAGSSSPTLSIAISCVGRRLVLGERTE
                     EEIEATLEILPNGSRQVGFYSYGEISPYASGACDLHNQTMTLTTITEL"
     gene            complement(10509..12248)
                     /locus_tag="PLS35_10180"
     CDS             complement(10509..12248)
                     /locus_tag="PLS35_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19076.1"
                     /translation="MHSFVSNLLAALPKGDALDDPAWSVRHRGILYILWLHVLGVPLY
                     GAYMGAGPSLYLGGGALLGAIAIAAQLPAVGRRLQAAIATYGLVTASALLVHLSSGRI
                     ELHFHFFVMMSVIVLYQDWLPFLVGLQFIVIDHGILGTLVPSMVYVHAEGQAHPWTWA
                     LIHGSFILAQCAALLYFWRVNEVAREEALQSEARTRMIIETALDAVVTTNVSGIITDW
                     NTQAELMFDVSRTDAIGQPLTTYLSTAHSSTAADAGVVLSGLVPGAILNRRVESIGRT
                     GSGQEFPVEIATSCLAIGGTQQFTTFIQNISERKQHEEALCRAKEAAESASQAKSQFL
                     ANMSHEIRTPMNGVLGMTELLLATQLNDRQRRYADTAHTSGTNLLHIINDILDFSKIE
                     AGRLVLEHIPYDLRQLLAETATIFHEQAGKKGLSLTLAIAQDIPMTLHGDPHRLRQIL
                     TNLISNALKFTTTGRIAISAAMDQARPEHVRIEVSDTGIGIPEDAQARIFDSFAQADG
                     STTRKYGGTGLGLAIVKQLVGMMGGHIEVTSVPGEGATFWFTILAYATAARSTASDPS
                     TSSTEALRRLHHK"
     gene            complement(12440..13141)
                     /locus_tag="PLS35_10185"
     CDS             complement(12440..13141)
                     /locus_tag="PLS35_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19077.1"
                     /translation="MSQCVYCKQRKGKRSCPALTGLICSQCCGEHRLARISCPPDCEY
                     LDAGSDYQQKRLGEQFAPVRRELYRQLSVAGGEKAAALFNLIEVVTFGYFHDRRDGQD
                     AEVFAAIQALRRTLSPLHVPAAPMPVFAERLKKEYDTFVKQQPQQIADAGSAPEILDR
                     ALALLTEFSGQGFQSRRFLLGMIGYVKTFHPEIAEHLTKQHEAGRIVLPGQLTPPPQE
                     PAPHVHSPECRHHHH"
     gene            complement(13138..14160)
                     /locus_tag="PLS35_10190"
     CDS             complement(13138..14160)
                     /locus_tag="PLS35_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19078.1"
                     /translation="MTIFSFSSTRPRGRRNLGLTLAGAMGMVLASSWAWALQVSGLES
                     PHSFLADPPSRSYFISNINGDSNARDNNGFITKLSDDGRITAFKFIEGGRGEVTLHAP
                     KGMAVVDQVLYVTDVDTLRAFDKNTGKPVATVMLPRPADNKTTQSLVDVAADGKGHLY
                     LADQGGNAIYRVDLTSPLTLSLYVSGAHLAGPSGVAVNPKTGHIVVVSYDSGKIFDIT
                     PEGALTELASNGFFTGRFQNLSGVDFDRWGSMYVSDATKGKIWRMLPNNKFQVIAEYL
                     PGPSDLGIDRVNHLILVPYQDSNAAEVNGLESPTVSSGDRAKRTLADYGFGEPLKADK
                     EGSARK"
     gene            14423..15283
                     /gene="folD"
                     /locus_tag="PLS35_10195"
     CDS             14423..15283
                     /gene="folD"
                     /locus_tag="PLS35_10195"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNI19079.1"
                     /translation="MAARIIDGKALAQQVRERLAVDSAAVLAKTGTKPGLATILVGDD
                     PASHVYVKNKQKACELAGIYVDDHKLPASTTQAELLALIEKKNADPKIHGILVQLPLP
                     KHIESRVVLEAVSPNKDADGFHPYNFGRLVEGNPVFEACTPKGVIKMIESAGVSIEGK
                     RAVVVGRSNIVGKPLALMLLQRNATVTICHSKTKDLAAVCREADLLLVAIGKAKFVTA
                     DMVREGAVVIDVGTNKGPDGKLCGDVDYEPVSQKAGWISPVPGGVGPMTIAMLLDNTV
                     ESAKRMAGMK"
     gene            15441..>15846
                     /locus_tag="PLS35_10200"
     CDS             15441..>15846
                     /locus_tag="PLS35_10200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNI19080.1"
                     /translation="MSREPRRLKDVLAQGQFAVTVEYNPPKGTNITHVVESAKSLVGR
                     VHGVNVTDNTAAIVRAGSLPVCRILYELGHDPVMQLTCRDRNRIAMQSDLMGAHMLGI
                     RNILCLTGDYPTVGDHKDAKPVYDLDSVQVMQL"
BASE COUNT         3335 a         4228 c         4880 g         3403 t
ORIGIN      
        1 agttcatcga gaaaaaagaa atcaagaaga tggacacctt catccactat gcggtggggg
       61 cgagtcagct ggcagtggac gatgccggcc tgaaagtctc ggctgaagag gcgacgaggg
      121 ttggagtcta tattgggtct ggcatcggcg ggctgggatc gatcgaacat taccatgatg
      181 tgctgaagga aaagggcccc ggtcgagtct cgccattttt tattccgatg acgatcatca
      241 acctggcctc tggccaggtg gcgattcgag tcggcgcaaa aggtccgaat tcctgcgcgg
      301 tcacggcctg cgcaacgggg aaccattgca tcggcgacgc ctatcgcctc atcaagcggg
      361 acgatgcgga tgtcatgatt gccggtggtg cggaagcggc aattaccccg ctgggtgttg
      421 ccggatttgc gtcggctaag gccctttcgt tcaggaatac cgaaccggcg aaagccagtc
      481 ggcctttcga taaggatcgt gacggattcg tgttgggaga aggggccggc gtggtggtgc
      541 tcgaagaatt ggagcacgcc cgcgcgcgcg gtgcacgcat ctatgccgaa gtcatcgggt
      601 atgccatgaa tagtgatgcc taccatatta cggcgccgcc cgaagaaggt gagggtgcgg
      661 tgcgttgcat ggaaatggcg atcaaagacg ctggtgtggc gaagacggac atcggctata
      721 tcaacgccca cggcacttcg acgatggccg atgccatcga aaccaaggcg atcaagcacg
      781 tgtttgggga gcaggcctat aagattccgg tcagttcgac gaaatcgatg acgggccatc
      841 tcctgggtgc cgctggtggg atcgaggccg tattcagtat tctggcgttg caccatggta
      901 ttctgcctcc cacgatcaac cttgatcatc ctgatcccgc ctgcgacttg gattatgttc
      961 cgaataaggc tcgtgcgacc aagactcagg tggtcctttc gaattcattc ggcttcggcg
     1021 gggtcaacgc ctgcctgctt tttcgccggt gtgaccaata atcctcctca gagcacgagt
     1081 caggtggtat gacgtcggca acgtcaatcg aggcggtcca gcgcctgttg ggctaccgct
     1141 ttcgtcagcc gcgtctgctg gaagaagccc tgacccataa atcctactcg aacgagcgac
     1201 gtaccaaaga ccgcactcaa aacgagcgct tggaatttct cggcgacgcg gtcctgtcgt
     1261 tagtcatgag cgagtatctc gccgcagaat tccccggcag caacgaaggc gggctgtcca
     1321 aactgaaggc gcatttggtc agtgaggcgt ccctggccaa ggccgcccgt cggatgaagc
     1381 tcggccgtct gttgcggttg ggaaagggcg aggagctttc gaaaggccgt gaaaaacact
     1441 cgttgctggc cgatgccctc gaggcgttga tcgccgccgt ttacctggat ggcggcctcg
     1501 aggcgagccg gacctttacc ttacgtgtgc tggaagagga gttgctggcg gctcgcgcag
     1561 aacgggcccg gccgggaatg gacgattaca agacgcagct acaggaactt tgtcagaagc
     1621 ggtttgaaag tttgccgcag tatgcgacgg ttcgagaatc cggcccggac catgagaagg
     1681 tgtttgaggt ccaactgaca attcagggcg tgatgcgggg agtcggatgc gggcacagca
     1741 agaaagaggc agagcagatg gccgcgcggg aagcgctgac acaattaacg acatgagctc
     1801 ttcaaggaac cgacaagaag gaggcatgat gacacaatca cgatggcaca atcgatttgg
     1861 gtgcgcggca gtgatgagcc tggttctggc catcggcatg gctacaaccg gtctggcggc
     1921 tgatgctcct gccaaggcag gcgattccgt caatgcgagt aatgtgcgag cggtcatcaa
     1981 gaccaaggtt ggtgacattg aaatcaagtt tttgccggac gttgcgccta aacatgtcga
     2041 aaatttcatc aagctcgcga agtcaggatt ttataacggg acgatatttc atcgtgtgat
     2101 ccccgggttt atgattcagg ggggggaccc aaacaccaag gactcgttga gaaaagacgc
     2161 ctatggtcaa ggcggtcccg gacacaacgt caaagctgag ttcagcgatc tgccgcacaa
     2221 gcgaggcgtt gtctcgatgg cgcgggcaca ggatcccgat agcgcggggt cgcaattttt
     2281 tatcgtcgtg gaggaatcgc gtttcctgga ccggaaatac accatctttg gcgaagtggt
     2341 gaaagggatc ggagtcgcgg acaaaattgt ggcactcccg agagtcatgt gccaaacggg
     2401 tgccccgagt cctgcagaca agggcccttg cgataatccg attgatcgtg tcgagatgac
     2461 ggtgacgatt atcgagtgag gcgctggacg tgagcaccgg gccgatctgc cttgtacgat
     2521 cagcccgggt gctgacggtt ctgattagaa gggttgattc tgttgctggg tgcggacgag
     2581 ggtcaggaag tgagagaggg ctttggccat gcgctcagcg tgtgaggcgg agcgaaagag
     2641 caatgtgtcc tcaggaaact ggcctttcac ttccgtgacc gctccggtgc agaagctggt
     2701 tttcagaatg caggggccat tgctattgca tgatatgcgg accgcataca gcaccaggtt
     2761 attctcacgc gtgtcctgcc cctggtaggc aatgctctcg atgtcctgca gattgaaatg
     2821 agtaaagccg gtgttgacgt cgtcgtgccc gtggttcaac tcgatcgtgc cgagtctggt
     2881 tcctgttgga atgacactat agatttcggt caccttggtt ggtttggcgg ctttcggatt
     2941 ccggcaacta agacttcgca cgaagccgtg tgtattggcc atgtcgctga tgaactgtac
     3001 ggtgtcttcg atggattgac cgaaacctgt ggtaggcagc agcacgacgc agaaacatgc
     3061 cacggtcaga ccgaccctcc tgagccacca gccccataaa ttcgcctgtc tcttctgcat
     3121 gcttcgtctc ccttgtaggc tggtataggc tcgtccctca cgtgcgtacc gtactgaacc
     3181 tgccgaggca gtgcaatcct tacttttgca tagcacgtgg ccgctcgtga accgcctaga
     3241 tggcgatgct atctggttga gaaagcctgg cggtgacgag ggtcagacga cgtcttgggc
     3301 gatttcggct ggggtagaag ggaagacggt tggatcgata ggcaagatcg gggaaagatc
     3361 gatttgttca gcaatggagc cgaaccggaa gctgttcatg accgtggaca tgcgaaattc
     3421 catcttgtcc agattcaaat agtggaacat tttggaaagg aacgggcctt ccatatgtgg
     3481 ctgatacgga tcgagttccc acggatgaaa atacatgacg agaggccggc ctttttgttc
     3541 gatcctccgc atcagtccgc acagagcggg gaacggcaac agccgaaaat aacttcctcc
     3601 tgcaatgggt atccgcactc caccgagatt gaccgtggat ggcggaacct cccagatggg
     3661 acccgacgag gtttgtttca gatgctgcca gggatcagat ccaggaagcc cacaatgatc
     3721 gtgccggata gggacgatgc tggagtcgta ggtgtaacct tcttctgcca ggattggtaa
     3781 ggcccacagc gtttcacgcg taatggtaaa gccgggtgcg cggtatcctt ggacgggaga
     3841 tcctatcagg tcttccagga tctgtttggc ctgtctcaca tcagcgcgaa acaactccgg
     3901 ggtttgggct gtgactaatt catgcccata tccgtgagac gcaatttcat gtccgcattc
     3961 ggcaatctgc ttgatcaaac cggggtgacg ctcggcgacc catcccagaa caaaaaaggt
     4021 ggctttggtg tcatagcgcg cgagaagatc cagcaccttg caggtgttgg ctgtcacccg
     4081 actttcgaag gctccccagt gtcgtcggcg aatcggcgag tcaaatcgtg agacttgaaa
     4141 atgttcttcg atgtcgaatg tcaggcaatg cacagatgtc atccttgggt gtatggattt
     4201 gcctactagc tgggatgggt aagggtttgc atgctgacgc tggagaggta cccctttcga
     4261 cccgctttgt atactatgtg tacgcaatga agtaaagcaa agtttaagcc attacttttc
     4321 agtagtgatt tcaatgggat gactaacagt cccaagaagt cgaggccgta ggtgtatcag
     4381 agaagtctta caggaggtat ctgaaaagat acacaggcgt ctcttccata atcgtgagcg
     4441 cagagacgac agaaggggaa gaggaagcct gctgtcaggc ggctgatttg tttcgttcgt
     4501 tgtccgcgag tggttgctga agagggacag taaaccggaa gacggaccct gctccaggtt
     4561 cgctttccgc tccgatgtgt ccccccatca gttccaccaa ttgcttgcaa atcgcgagac
     4621 ccaatcccgt tcctccatac ttcctcgtgg tggatccgtc tgcctggacg aagggctgaa
     4681 acaacttcgc aaacgcgtta ggcgcaattc cgataccgtt atccgctacg gagaatcgca
     4741 cgtgactgat tcggctggca gtggatgaca gttcgccata actggtggcg ggaaactcga
     4801 gacgctgttc agacggccgc tggtcaagat cgacggtaag agagaccgtt ccacgatcac
     4861 tgaatttgat ggcgtttgcc agcaggttgc tgaggatttg ttggagtcgg gtcggatctc
     4921 cgcataaagc agccgggacg tcgtcggcaa tctgcgaatg aaggaggaga ccctatcgtt
     4981 gtgctcgctc tgcgaataac gccaggactc gatcgacaaa cttcggcaga tcaaggtcga
     5041 gcaattcgag agacagtttg ccggattcga ttttggaaaa atcgagaatg tcgttgatga
     5101 tgaccatcaa ggaatcgccc gaggaccgga tcgtttgcgc gcaatccagc tgttccgccg
     5161 tcagttcggt ttccaacagc cagtcggtca taccgagtac gccgttcatc ggcgtgcgga
     5221 tttcatgact catggtcgca aggaattcag atttgaggcg ggccccttcc atcgctgcat
     5281 cgcgagcctg cgtcagagtc gcctgactgg ctcggagttc ctggagtacc tggttggctc
     5341 gaaccatata ccggatgcgg tgagtcagca acatggcgtt caagggcttc acgataaaat
     5401 ccgtcgcgcc cgcatcatag gcttttgtaa tggattcaaa atcgtccaaa ccagtcatga
     5461 tcagaatcgg ggtgtgttcg ccgcctggta actgtcgtaa ggcggcacag gtctgaaacc
     5521 cgtccatttc cggcatcatg acatcaagca gtacgacatc cggcgggcac ttcttaaagg
     5581 ccgcgcaggc ttcgagtccg ttctcagcct cttcgacctc ccatcctgct tgttccaggg
     5641 cctcccgtgc aaacatgcgg atgatgatgt cgtcgtcggt gactaatgcg tagggtgacc
     5701 gtcccgaggt gagcgggttc atgatgattt ctccttggcg atctcgtttc gaaagacggc
     5761 acaagcggca gcatattcgg attgtaattc gaccaaaata cgatcggctt cgatcagatt
     5821 tttttgagcg cccagcgcct ccagttcctt acagcgggcg gctaccgcaa gggctccaag
     5881 ctgagcgctg ctcgatttga ggcgatgcgc cactgcttgc aagccagcgg gatcgttcgc
     5941 acgtatcgcg tcctgtaagg catctacact gtctcgtgag ttatcgagat acttgcgaag
     6001 aacggacgcc agcacatcag gccggtttgg ccgttgcaag gcccgaatgc cgtcgagagc
     6061 tttaggatcc atgctcggag tgctcggtgc gggggcagct gcagcggggg ctgattcgcc
     6121 agataacgcc tgcgttgagc tgaagactgg ggcgagcgct cgatcagtcc cttgcttgtc
     6181 taaccatttc tgcacgacgg tctgcaattg cacctgcgtg tagggtttgg tcagatagtc
     6241 atccatgccg gccgcgagac attgttcccg gtctccttgc atggcattgg cggtcaaggc
     6301 aatgatggtg agtcttcgct ggccggatgc cgcttcacgt cggcgaattt ctcccgtcgc
     6361 ggtaaaccca tccatctcag gcatctgaca atccatgagc acgaggtcaa actgactgcg
     6421 cccggccaat tccaatgcct gccggccgtt ttccgccatc tcgacctggt acccgaggat
     6481 ttccagcatg ccgacggcga cttcacgatt caccgggcta tcttcagcta tgagaatcct
     6541 ggccacacgg tgtcccgttc tcttggcctc cgttccaggc gatgacgggt atggctccga
     6601 cggcccatag ggcggttcga gagtaaggtg cggcttagca agtcgctcat aggatggcag
     6661 gccgttgtcc gttcccgtgc caagcggttg cagttcgaat cgggccgtga atgagaatgt
     6721 cgagccacgg cccggttcac tttccaccgt aatcgctcct cccatgaggc ccaccaattg
     6781 tttggcgatc gacaaaccga gacctgttcc accgtaccgt cgggtggtcg aaccgtccgc
     6841 ttgcgcaaag gcatcaaaaa ttcgcaattt ggctgcgggc ggaataccga tgccggtgtc
     6901 cgtgaccgaa atgcggagtt gcgcatgagt gggagtgcca ctcatgtagt cggccgtcag
     6961 cgacaccccg ccggtttcgg taaacttcat ggcgttgctc aacagattca tgagaatttg
     7021 acggagtcgg accggatccc ccatcaaata acgcgggacc cgttcatcga cctgttgagt
     7081 gaggtgaagg tgtttccgcc gggcggcttc catgaacagc tcgagggatt cgtcgagaag
     7141 ttgcgacaga tcgaatccga cacattccag atccagtttg ccggcttcga tttttgagaa
     7201 atcgagaata tcgttaatga tggcgaggag cgttcgtccc gaccggtgca cggtcgacgc
     7261 gagatggcgc tgcttatcgg tgagggtact gttgagcaat agttcggtcg tgccgagcac
     7321 gccgttcatc ggcgtacgga tttcatggct catattggcg aggaattcgc tcttcgcctt
     7381 gctggccgcc tctgcggctt cttttgccgc gcgaagttcc tcctcagtac gtttccgatc
     7441 ggtcgtatcg atatgaatgc cgaccattcg cgcggccacg ccatagacat cgcgtatgag
     7501 ggtgccgcgg gataaaatcc atcggtagga tccatctcga tgccgcaggc gatgctcgag
     7561 ttcgaattgg gcgcggtcgc cgttcaggca agagtggacg gtctgatgga ccagcgcccg
     7621 gtcttccggg tgaatgcggg attgccactc ctggaatgcg ttgggcaatg tggtatcgtc
     7681 gtggccgagc tgactcttcc actgcggcga aaaatagact gaatctgtgg tgaggttcca
     7741 gtcccatatg ccgatgtgag acccttgcac ggtcatcgtg aggcgggctt cactggtgcg
     7801 cagggcttcc tccgcctgtt tccgctcgga aatatccacg acgaaggctg tgaaggtata
     7861 ggcattttcc agacggagcg gggtgatcgc gagttcgatc ggaaattccg tgccatctct
     7921 gcgcagcgcg ctaatttcga ttcgtttatt gaggatcggt ccctcgccgg ttttgaggaa
     7981 ctgccttatc cctctctggt gagcctctcg ctgagaggcc ggaataatgg tctctgccat
     8041 caagcgaccg atggcttcct ggcgcgtcca cccgaatgtc tgctccgcct gcgtgttcca
     8101 gccgataatg cgtccctgct cgtcgatgct gatgactgag ctgagcgccg tgtcgataat
     8161 cagcctggtc cgcccttcgc ttttgcgcag cgccccttcg gcgtggagcc gctcgaccgc
     8221 ttctagcgcc aaggacacta acgaggcgac ggcataactg aattgttgtt cctcgagcat
     8281 ccaggagcgt ggcgtgccta cgtgttcatt gcacagcact cctaccagtt tccctttgaa
     8341 gcgaatcggg atatcgagca acgagctgat ccctaacggg gtcaggtagg tgtcggtgaa
     8401 ttcggacgtg cggggatcgg tccgcgcgtc cgaggcgtct atgacccgtt ccgtgaggag
     8461 ctcccggaaa tacttgggga attgcgcggc gaggagttcc atcccgcatg aatggctgtt
     8521 gttggacgcg tcgtggagat ctttacagat aatggcgcta tggtcgtctc gcagaagcca
     8581 gatgctgcac cggcgcaccc cgagggtcgc ggctgtcacc cgggtgattt ctcggagggc
     8641 cggctcgagc acgccgcttt gaatgatgct gttgctggtt agaccaaaca gtgcggcctg
     8701 atgttgccgc aacaaactct ccgtatggcg acgaacatcc tcggcctgct tgcgctccga
     8761 aatgtcccga aagaccagca ccgctccacc ggcctgaccg tcccgcacga taggagccaa
     8821 cacgcaggaa acgggaaacg aacggcccgt gatcgacgtg agtatgccat catcggttct
     8881 gaaggaatgt ccttgagccg tttcatccag cagcatctgg gtaaagatcg gctcggtgag
     8941 attggtgccg tacgagaggg aaatcatatc gggcaggcgt cgtcccacga cgtccgcttc
     9001 gggaagtccc cacaaacgct gcccctcagg attgagcagg acgatgtccc agtttcgatc
     9061 cacgacgcac agtccgtcac ccatggaacg aagcacggtg tggagtttgt cccgctcctg
     9121 ggtcaactga ctttctgatg tgcggcgaag ctgctcgttc agttcctgca tctcgctggc
     9181 ggacagggca atcgagcgtt cgagcagttc gcgtccctgg tcggactcga tgtagctccg
     9241 gctgacgcgt tcaagcagtt gctgccaggc ctcaagcgag gtgggggcgg cggtagtctc
     9301 cagtcccaac cgcttgagtt gtcgtgtgag cagcggatgc atacagatta caattcggta
     9361 atcgtggtca acgtcatggt ttgattgtga agatcgcagg ctccgctggc atagggagaa
     9421 atttccccat aggaatagaa tccgacttgg cggcttccat tgggcagaat ttccagcgtc
     9481 gcttcgattt cttcttccgt tcgttccccg agcaccaacc gccgtccgac gcagctgatc
     9541 gcgatcgaca gcgtggggga agagctgccc gcaggttcat gattgttcag ggtgagggtt
     9601 gccgcttcag acgctccctg aatcaagcgg tcaaagttgg cacgcatcaa ctgggcgagc
     9661 atgccctccg gtaaatcccc cgcgaaggtc atggattgcg ctgcttcatc aacggccagg
     9721 atggtccgga ccaggacttt cccatcggtg ttcgaagatc tgatggcgag ggggaagagc
     9781 aggccggtgg ccgggagccc ggcggctcgg tcaccgagat actctttata gagttgcagg
     9841 gcagggcgcc catcgagttc gtagagaatg ttgcctgccg atttagtcac ccgccgctca
     9901 gggccgaact tatcccatcc gccctttgag ccatggccca agcggacatg gtcgccatag
     9961 aagccgatgg cggtgacata cccgctctgc ggcgcacggt ctttgatgac ccatgtccgc
    10021 ttgaagtcgg ttccatcgcc ggccaaaccc ccggtcacga caacggagcc tcccagggtg
    10081 tcattcagtc ccttcacgag ttcgctgcca ttgacattca acccatcaga gagcactaac
    10141 acgcctctga gtgagggctg atgaaggctc gcggcaatgg cgcgtccggc cgcgtacgaa
    10201 tctttggggg tgtgaaccgc cgcctgggtg gtgaggaggc gcgtcttctc aaagcgcacg
    10261 gccgcgacga ccaggctgtc atccgagatt tcaacaccat gaatttctcc cgccgtagag
    10321 cagcccatca catggcttcg agggcaagcg gccatcactt gctgaatgag ctccggtcga
    10381 tcaatgagac ttgaggatcc gaacagcagg agaagactgt tctccgaatc gtgggcggcc
    10441 agttcagcag gaatatcggc atgaccggct tttagagagg tggttgcgac atgcatggga
    10501 tgttctcctt acttatggtg caggcgccgc agcgcctccg tggaagaggt ggatggatcg
    10561 gaggcagtcg atcgtgctgc ggtggcatac gcgaggatcg taaaccagaa ggtggctccc
    10621 tctccgggca cactggtcac ttcaatatgc ccgcccatca tgccgactaa ttgcttcaca
    10681 atggcgaggc cgagcccggt cccgccatat tttctcgtag tcgatccgtc agcttgcgca
    10741 aatgaatcga agattcgagc ctgagcgtct tctggaatgc cgattcccgt gtcggacacc
    10801 tcgatccgta catgttcagg ccgggcctga tccatggctg ccgagatggc aatccgtccg
    10861 gtggttgtga atttgagcgc attgctgatg aggttcgtga ggatctgccg caggcggtgc
    10921 gggtctccgt ggagggtcat ggggatgtct tgcgcaatcg ccagggtcaa cgacagccct
    10981 tttttccctg cctgctcgtg aaagatggtc gcggtttcgg ccagcagttg gcgcaagtcg
    11041 taggggatgt gctccaagac caagcgtccg gcttcgatct tcgaaaaatc caggatgtca
    11101 ttgatgatgt gcagtaagtt ggtcccggat gtgtgcgcgg tgtcggcata tcgtcgttgc
    11161 cggtcgttga gctgcgtcgc gaggagtaat tccgtcatgc cgagtacccc gttcatcggg
    11221 gttcggatct cgtgactcat attagccagg aattgggact tcgcctgact ggccgattcc
    11281 gccgcctcct tggcccggca taaggcttcc tcgtgctgct tgcgttcgga aatattctgg
    11341 atgaaggtgg tgaactgttg agttcctccg atggcgaggc agctggtggc gatctcgacg
    11401 ggaaattcct ggccggaacc ggtccggccg atgctctcca ctcgacggtt gagaatcgct
    11461 ccgggaacca gtccggaaag caccacgccg gcatcggccg cggttgaaga atgggccgtg
    11521 gagagatagg tggtcagggg ttgtccgatg gcgtcggtgc gggatacgtc gaacatcaat
    11581 tcagcctggg tgttccagtc ggtaatgatg ccggagacgt tcgtcgtgac gacggcgtcg
    11641 agggcggttt cgatgatcat ccgggtgcgg gcctcactct gcagggcttc ctcgcgagcg
    11701 acctcgttga cacgccaaaa atagaggagc gctgcgcatt gagccagaat gaagctgccg
    11761 tgaatcagtg cccaggtcca cgggtgcgcc tgcccttcgg catggacata caccatgctg
    11821 ggcaccaggg tgccgagaat cccatggtcg atgacgatga actgcagtcc aaccagaaac
    11881 gggagccaat cctggtacag cacgatcacg gacatcatca caaaaaaatg aaagtgcagt
    11941 tcgatgcgtc cgctggagag gtgcaccaag agcgccgagg ctgtgaccaa tccataggtg
    12001 gcaatggcgg cctgaagccg acgaccgact gccggaagct gtgcggcgat cgcaatggcg
    12061 ccgaggaggg ctccaccgcc gagataaagg ctgggcccgg cgcccatata ggcgccgtag
    12121 aggggaacgc caagaacgtg caaccagagg atatagagaa tgccgcggtg acggacggac
    12181 caggccgggt cgtccagcgc atctcccttc ggcaaggcgg ctaagagatt actgacaaat
    12241 gaatgcatga aatttcaatg cgttgcgcgc gcagtccgga tttgtgtctt gcgcaggtgt
    12301 aaatcctggc ccgactgtat cggcagaaac ggggtgtaac taaagggaaa acgagggaaa
    12361 gcgaaggggg aatctagcgg tagacaacct gctcaaacgg cgaatgggcg tctctcatgg
    12421 ttgggcgagc ggcaggtgat cagtggtggt gatgacggca ctccgggcta tggacatggg
    12481 gtgcgggttc ctgaggcggc ggagtcagct gcccgggcag cacaatgcga ccggcttcat
    12541 gctgcttggt gagatgttcg gcaatttcgg ggtggaaggt tttgacatag ccgatcatgc
    12601 caagaaggaa gcggcgtgat tggaaccctt ggccggaaaa ttccgtgagg agcgccaacg
    12661 cgcgatcgag aatttccggg gctgagccgg cgtcggcgat ttgctgcggt tgctgcttga
    12721 cgaacgtgtc atattctttc ttgagacgtt ccgcaaagac cggcattggc gcggcgggca
    12781 catggagagg actgagggtg cggcgcaacg cttgaatggc tgcgaagact tcggcatcct
    12841 gcccatcccg ccggtcatgg aaatagccga atgtcacgac ttcgatcagg ttgaacaagg
    12901 ctgccgcctt ttcgccgccg gcgacgctga gctgacgata gagttcccga cggaccgggg
    12961 cgaactgttc gccgagccgt ttctgttgat agtcgctccc cgcgtcgagg tactcgcaat
    13021 caggagggca ggagatacgg gccaggcgat gctccccgca acattggctg caaatcagcc
    13081 ccgtgagggc cggacaggaa cgtttccctt tgcgctgctt acagtagaca cactggctca
    13141 tttgcgggcc gatccttcct tgtcagcttt cagaggttca ccaaacccgt aatccgccaa
    13201 ggtgcgtttg gcacgatcac cagatgacac cgtgggagat tcgaggccgt tgacctcggc
    13261 cgcattcgag tcctggtacg gcacgagaat caagtgattc acgcggtcga tgccgaggtc
    13321 tgacggcccg ggcagatatt ccgcgatgac ttgaaatttg ttgttcggca acatgcgcca
    13381 gatcttgcct ttggtcgcat ctgatacgta catgcttccc cagcgatcga aatcgactcc
    13441 gctaaggttt tgaaagcgtc ccgtaaaaaa tccgttggag gccaattcgg tcagggctcc
    13501 ttccggagta atgtcgaaaa tcttgccgga atcataactc accacgacga tatgccccgt
    13561 tttgggattc acggccactc ctgaaggacc ggccaaatgg gctccggaca cataaagaga
    13621 cagcgtcaat ggggaggtca gatcgacccg ataaattgca ttgccgccct ggtcggcgag
    13681 atacaaatgg ccctttccat cggcagccac atccacgagc gactgggtgg tcttgttgtc
    13741 ggcgggccgg gggagcatta ccgtcgcgac gggtttgccc gtgttcttgt caaacgcgcg
    13801 cagggtgtcg acatccgtga catacagtac ctgatcgacg acggccatgc ctttgggggc
    13861 atggagcgtg acctctccgc gacctccttc gatgaacttg aaggcggtaa tgcgaccatc
    13921 gtcactcagc ttggtaatga acccgttgtt atcccgggcg ttgctgtccc cattgatgtt
    13981 ggaaatgaaa taggacctcg aggggggatc agccagaaag ctgtgtggag attccaatcc
    14041 gctgacttgc agggcccagg cccatgagga ggccagcacc attcccatgg cgcccgcaag
    14101 agtcagtcca aggttgcgac ggcctctcgg ccgtgtcgaa gagaaggaaa agatggtcat
    14161 ggaatcatca acgaagatgg tcgcacagat tgtcctgatg catcgtagcc aagggtctcg
    14221 gaacctgtca aggaagcagc aacatcgcgt atgagcgagc ggagaaggcc ggacggacag
    14281 acaacggcac gaccttgcga ggtccctgtg gaatcgaggg ctttcatcag atgcgtcccg
    14341 ccgcctccag cgttgacttg ttgaaaaatt gcctgctatg gtgccctcac ttttcgcttc
    14401 tactcaggcc agggaggata ttgtggcggc acgaattatc gacgggaaag cattggcgca
    14461 acaggtgcgt gaacggttgg cggttgactc tgcggcggtt ctggctaaaa cgggcaccaa
    14521 gccgggcctg gccacgatcc tcgtgggtga cgatcctgcg tcgcacgtct acgtcaagaa
    14581 taaacagaaa gcctgtgaac tggccgggat ttatgtggac gaccacaagc tgcccgccag
    14641 tacgacccag gcggaacttt tagcgttgat cgaaaagaag aacgccgatc cgaagattca
    14701 cggcatcctg gtccagctgc cgctgcctaa gcatatcgag agccgcgtgg tgctggaagc
    14761 ggtctcgccg aacaaggatg ctgacggatt ccatccatac aacttcggcc ggttggtcga
    14821 aggcaacccg gtgttcgaag cctgtacgcc gaagggggtc atcaagatga tcgaatcggc
    14881 cggggtttcc atcgaaggca agcgcgccgt ggttgtgggg cgaagcaata tcgtgggcaa
    14941 gccgttggcg ttgatgctgc tgcaacgcaa tgccaccgtg acgatttgcc attcgaagac
    15001 gaaggatctt gccgcggtct gccgcgaggc ggatctgctg ctggtggcga tcgggaaggc
    15061 caagtttgtg acggcggata tggtacgaga gggggcggtc gtcatcgacg tgggtaccaa
    15121 caaggggccg gatggcaagc tctgcggaga tgtcgattat gagccggtga gccagaaggc
    15181 cgggtggatt agtccggtac caggcggcgt cgggccgatg acgattgcga tgttgctcga
    15241 caataccgtt gaatctgcta agagaatggc aggcatgaaa taggcagatc gggccgcgcc
    15301 gttgctcgca gaccgcgtac gatcagaatg tgctcggtcg atgcgcgtaa tcgggacgtc
    15361 ccgacccgcc tctttcaagt gaggtagggg aagatgactc ggaggatagg gagaccacaa
    15421 accatgcagg gaagatctcg atgagccggg agccgcggcg actcaaggat gtgctggcgc
    15481 aggggcaatt tgccgtcacg gtggaatata acccgccgaa gggtaccaac atcacccatg
    15541 tggtcgagag cgcaaagtcg ctggtcggcc gcgtgcacgg ggtcaatgtc actgacaaca
    15601 ctgcggcgat cgtgcgggcc gggtcactgc cagtgtgccg gattctgtat gaactgggcc
    15661 atgacccggt gatgcagttg acctgccggg accgcaatcg catcgcgatg cagtcagacc
    15721 tcatgggcgc ccatatgttg ggcatccgca acatcctctg cttgaccggg gactacccca
    15781 ccgtggggga ccataaggac gccaagccgg tgtatgacct ggattccgtg caagtcatgc
    15841 agttgg
//
LOCUS       DAOQEW010000081        15591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1044_length_15591_cov_9.103566, whole genome shotgun sequence.
ACCESSION   DAOQEW010000081 DAOQEW010000000
VERSION     DAOQEW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15591
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1044_length_15591_cov_9.103566"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLS35_10205"
     CDS             complement(<1..748)
                     /locus_tag="PLS35_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNI19081.1"
                     /translation="MQKTSPTKVIIDTDPGVDDALAILLALASPELDVVGITTVCGNV
                     PVGQGTKNLFRLLNLLNPPPGLLVGQGAARPLEEALETAVHVHGSDGLGELDALLSAE
                     GTMRYPAVRLPPLLSTAQDVWNECVRRYQDDITLITLGPLTNVAVALKVNPLTVQKFR
                     SVIVMGGAIGVPGNVSPVAEFNMYVDPHAAHRVFQAALPLTLVPLDVTTRVGLRRDVL
                     KMWTGASRDPRCRIVADMTAKAFDFAEQVEG"
     gene            complement(825..1511)
                     /locus_tag="PLS35_10210"
     CDS             complement(825..1511)
                     /locus_tag="PLS35_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19082.1"
                     /translation="METATSFAPVRRWMPSRDSASGGGTWLTAVGRVRRVVRQIAIGC
                     LLLNMFALPVHATVESVAVADTHIEDSPLDVVSGLTYRAGTRLRIPGTGWSFVVPEGW
                     QSNRPDDAEMPFLIPEEGKELGMMFPLAKVTRDALREQLREPLLLLHGLSFIPAGAQA
                     ETDAWIAQSYEGEGMAGRALAVMGADNTAVIYFFMGPPHNMSVLQSVLEQLGQSTRFV
                     QSTPRRDAGL"
     gene            complement(1459..4344)
                     /gene="gcvP"
                     /locus_tag="PLS35_10215"
     CDS             complement(1459..4344)
                     /gene="gcvP"
                     /locus_tag="PLS35_10215"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNI19083.1"
                     /translation="MPEPQFLEPNDTFVPRHIGPTESEIQTMLETVNVPSLDSLVEAT
                     VPSDIRLQGSLAVPSPRGEQEVLAELRGMAGQNQVWRSLIGMGYYDCITPPVIQRNIL
                     EGPGWYTQYTPYQAEIAQGRLEALVNFQTMVADLTGLPLANASLLDEATAAAEAMTMC
                     AAISRAAGHDRHKFFVSETCHPQTIAVVQTRAEPLGIVLQVGAIQSLDLSSGEFFGML
                     LQYPSTDGYVGDYSEFITRAHAAGVYVVVATDLLALTLLRPPGEFGADVAVGSSQRFG
                     VPLGFGGPHAAFMATKEEFRRQMPGRIIGVSKDVTGRPAYRLSLQTREQHIRREKATS
                     NICTAQVLLAVMAGMYAVYHGPAGLRRIAERIHGLTMALAEGLRRHGCAVGLEPVFDT
                     LRVPLSPAQSETIVNRARQQKINLRRYDDHSLGLSLDEWSTVDEVRQLLTLFAGQEIS
                     PEELQTILESVDGRYPAGLARTSQYLTHPVFHRYHAEHELLRYIHRLQSRDLSLVHSM
                     IPLGSCTMKLNATAEMLPVTWPEFGRLHPFAPADQVQGYLALFQQLESWLAEITGFAG
                     ISLQPNAGSQGEYAGLMVIRAYHRQRGETQRDICLIPVSAHGTNPASASMCGMTVVPV
                     ACDQRGNVDLADLEAKATQHRSRLAALMVTYPSTHGVFEEGIRRMCQIVHTHGGQVYM
                     DGANMNAQVGLCRPADLGADVCHLNLHKTFCIPHGGGGPGMGPIGVARHLMPFMPGHP
                     VTKLGGPQSIGPVSAAPYGSPSILTISWVYIALMGREGLTKATQVAILNANYMAKRLE
                     KFYPVLYSGARGFVAHEFILDLRPLKDSSGVEAMDVAKRLMDYGFHAPTVSFPVAGTL
                     MIEPTESEVKSELDRLCDALIAIRGEIQAIAEGRQPRAGNVLKNAPHTAMAVTASEWT
                     RPYSREEAAFPASWVRENKFWPSVGRIDEAYGDRHLFCTCPPVDAVS"
     gene            4552..6204
                     /locus_tag="PLS35_10220"
     CDS             4552..6204
                     /locus_tag="PLS35_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNI19084.1"
                     /translation="MTHDILVVGAGLAGMRAAIAVPPDLNVALLSKVHPIRSHSVAAQ
                     GGINAAIGAEDSWEAHAYDTAKGGLYLGDQDAIEAMCKEAPQDILELERMGVIFSRTA
                     EGRIAQRPFGGAGYPRTCYAADRTGHALLHAMYEQLMKRRCKVYEEWYVTALLVEEGR
                     CCGVVAWDMIHGGLQMLRAKAVILATGGSGRIFSTSTNAVINTGDGMALAYRVGIALE
                     DMEFVQFHPTTLKDTGILITEGARGEGGYLLNTLGERFMKRYAPEQMELATRSTVSLA
                     IGQEIQEGRGVDGCVLLDLRHLGRAKILERLPQIRELAMEFAGLDPIETPIPIRPGAH
                     YQMGGVRTNAWGETELPGLFAAGECACVSVHGANRLGGNSLLETIVFGRRTGTKAAES
                     IRTVQLSPVSETILKSEQQRIDKLLSNPGPERAWQIRDDLGKTMSLNLGIFRTKQSIQ
                     EALSAVQALKRRAEDMCVQDKGRVFNTDLIHALELQCLLEIAETIVVGALGREESRGA
                     HYRGDFPTRNDTAWLRHSLSYRRPEGPHLAYAPVTITRFPPA"
     gene            6380..7690
                     /locus_tag="PLS35_10225"
     CDS             6380..7690
                     /locus_tag="PLS35_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNI19085.1"
                     /translation="MTTVHPGTALPASPTLTASDAAQTAICPFCQAITSCAPVAGHRL
                     HQFPCPSCHANLLVLNPAARPTDEDKKTRSSDGDLPIDLSHRLTGIAVAVLACALNYV
                     LLMGMAYWILGQVKTTHDPYDIDALMLSPLILKANGWTPLHLAAARGDQSAVATLLTE
                     GQTVDQRNGNGRTALFEAAKRGQTDVVTLLLQHGANPSAKGKQGFTPLLAAAEQGHAD
                     TIEVLLTNGADIRATCTCGDSALHRAVRHGHLAAAQTLLEGGIPVNQKSHGETPLEIA
                     QHDEDHELIALLRAYGGREFSDAKAKRAEGIAFQKHGQIDHALFAYAEALNLDPDDHE
                     AYYGRGTALLQKNAPDEALIAFHAAIRLNPTYLDAYSAAASVHTSRGQWDQALRLWDQ
                     LLSHQPQNGRAIFERSIVKRGKGDSAGFLQGLQQACALGHRAAC"
     gene            7943..8965
                     /locus_tag="PLS35_10230"
     CDS             7943..8965
                     /locus_tag="PLS35_10230"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNI19086.1"
                     /translation="MPAPMRASFHGMGGTLLGMHVVNVCLTLVTLGGYHFWAKAKIRR
                     YLFSHTAFAGDRFAYHGTGKELYQGFLKAMLVFGIPYFSLSAAHTFLELPQWIDVLLQ
                     VMAGLVFFLYVPVAIVNARRYRCTRTSWRGIRFSFRGRTSDFLKLYFRGWFFTILTLG
                     TYYPYFQTQRQAFLHSHTYFGNQRFRFTGHGSGVMVPFAITLFTTYAVLALCGFALAA
                     QLTNAGLTLLLIPCVLGPMWIWLLGQKQRYFWNHTSFGDAQFSSEITWQRLFTLYLGN
                     LGLLLATLGWAWPWVTVRNARFFMGTLSLQGPVDLDRVLQDTSEASVTGEGLSNLLDT
                     GFDMDA"
     gene            8968..10110
                     /locus_tag="PLS35_10235"
     CDS             8968..10110
                     /locus_tag="PLS35_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNI19087.1"
                     /translation="MSTARTAHYLDGRTATRHRVTLTISPAALQIAMPDGSTKEWPYG
                     QIRQTQGTYRGEPVRLEFGTEPAEAVVVASRALLTDIHTAAPALAQHFHDPSWRQRRL
                     AWTLGAGLGVIVMLIGLYRWGIPGIASAATPYVPIMWEESLGRQIVQHLAPETQQCRE
                     PERLRKIDQVVQTLAATHPDSPYRITLSVVDNPAINAFAAPGGQVVILRGLLEQTTSA
                     EQLAGVLAHELQHVYLRHSTRAILEQTAGTLLLTAVSGDLSGGLAWGLQGAQTMGSLH
                     YSRTHEEEADVEGLRMMQAAHLDPTAMIAFYGTLQKSEQDHIEPPDFLSTHPDMSRRL
                     ATLLALAGPPPTDARQLLPGEDWKDIRTLCRLQTATRSSSDSPDLP"
     gene            10179..10850
                     /locus_tag="PLS35_10240"
     CDS             10179..10850
                     /locus_tag="PLS35_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI19088.1"
                     /translation="MRVLVVEDETKVGSFIKRALEEESYAVDLCEDGAQGLDLALTGS
                     YDLIMIDLMLPGLPGMEVLTRLRKEKIQTPVLILTAQSKVDQRVKGLDAGADDYLTKP
                     FAIDELLARVRALLRRGTAESSGTLQIDDLILNPATREVTRGGQRIDLTVKEYALLEY
                     FMRHAGRVLTRPMISDHVWNQDFDTFTNVIDVYVNYLRNKIDRGRARKLIHTIRGSGY
                     MLKVD"
     gene            10850..12253
                     /locus_tag="PLS35_10245"
     CDS             10850..12253
                     /locus_tag="PLS35_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19089.1"
                     /translation="MPLRVRLTLWYGTALALILLTFSVVLYTITARGLRDQVDESLQE
                     TASAAVRSLETRGFLPLIDEDALLSQFPELTRIDKFFQIFSPTGTITIRSPNIRQHDV
                     PLSRPALEAAFTGSTVFESARYPNEPPLRLVSVPIIYRGSLLYIVQVGTTLEGVEETL
                     RRFLLVLLVMAPIALVVSLVGGWFLAGRALRPVDSITVAAQRIAAGDLTQRLHSERST
                     DEIGRLTDTFNNMIARLETSFAQIRQFSSDASHELRTPLTVMKGESELVLRRPRPVED
                     YIAVLESNLEEIDRMSRIVEELLFLSRADMGQVKTECEPVRLEALVEDIQRQACLLGQ
                     EREVDVIMGTIQPATVRGDELRLRELILNIVDNAVKYSHPCGKVEIDLRVEGSTARLA
                     VRDYGIGIPQDAHKQIFDRFFRTDDARAHTKKGTGLGLAICAWIAEAHHGHIEVQSEV
                     GKGSTFTLVLPLAATAA"
     gene            12372..13850
                     /locus_tag="PLS35_10250"
     CDS             12372..13850
                     /locus_tag="PLS35_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNI19090.1"
                     /translation="MKRPTPSVSVLAGIAAVSAGLIWSYTPLSPSHASNPSPVVEERP
                     IAPTGALPAVGFADVAKTVTPAVVNITTSGAEEVSDHGRPRGKMDDFFGSPFGPRRFG
                     PPTEPREHRGGGQGSGVIVTQDGYVLTNNHVIAGAKTVTVTLPDKREFKGRIVGSDPK
                     TDIAVVKIDGTQLPTVPWGDSSRLQVGEYVLAVGNPFGLNSTVTLGIVSALGRGHMGI
                     TQYEDFIQTDAAINPGNSGGALVNTKGELIGINTAIFSQTGGYQGVGFAVSTGMSKPI
                     YESLVKTGKVVRGYLGIGIQDLNHDLAKSFGVKGTNGAVVTDVKEEGPADKAGIKQGD
                     VILSFQGTPIEDAVTLQRAVTRTAVGSKATVTVLRDGQEKELAVTLAELPENQQVAKT
                     ETEPSDQPLAGLAVQELDRETAQELGLKGKIQGVVVTTVDPESDAERAGLMPGDVIRE
                     INRKPVTSMKEFDRAASSLKKGQTVLVLINRRGASLYLSAKI"
     gene            complement(13893..15347)
                     /gene="rimO"
                     /locus_tag="PLS35_10255"
     CDS             complement(13893..15347)
                     /gene="rimO"
                     /locus_tag="PLS35_10255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNI19091.1"
                     /translation="MASSLITPSRAKGKKPTIGFVNLGCSKNQVDSEVMLGTLVAGGF
                     QLTGDARAAEVVIINTCGFIEEAKQESINSIIEHGRLKKSGSCRVLIAAGCLAQRYQG
                     ELLKELPELDGVVGTGEFGRIADICRSLLAPKKRQQRLWLGQPPYLYDADTPRVRLGT
                     PHSAYLKIAEGCNRNCAFCAIPIMRGKQRSRPIDSIVAEARRLAKEGVKELNLISQDT
                     INYGVDLGLKQGLTALLRELVTVDDVRWIRPFYLYPQQVTDELLELYAGEARITKYLD
                     MPLQHISDGMLKRMHRLGDRKHLTKLVERIRTKIPGVFFRTAFIVGFPGETDKQFEEL
                     KQFVLDMEFDRVAVFLYSDEEGTSAVDLDRKVDQAVMEERRNELLALQESISESKNRE
                     YLGRTIEVLVDGVSEESDRLLEARHQGLAPEIDGVVYCERGIARPGEFISVTVTDVAG
                     YDLIAQPAGQTHQPNRSSDSPGLLMPKKAGAGYR"
BASE COUNT         3297 a         4868 c         4407 g         3019 t
ORIGIN      
        1 gcccttcaac ctgttctgca aaatcgaatg ccttggcggt catgtctgcc acgatacgac
       61 agcgtggatc gcgcgaggca ccggtccaca tcttcaggac atcacgacgc agcccgacac
      121 gggtggtcac gtccagcggc accagggtta atggaagcgc cgcctgaaac acccgatggg
      181 ccgcgtgggg atcgacatac atattgaact cagccaccgg tgatacgttg ccgggcacac
      241 cgatcgcacc ccccatcaca atcacggaac gaaacttctg gacggtaagt ggattgactt
      301 tcagcgccac agccacattc gtcagcggcc ccagggtaat aagcgtgatg tcgtcctgat
      361 accgccgcac acattcgttc cacacatctt gggcagtgga caggagaggc ggtagacgaa
      421 cagcggggta ccgcatcgtt ccctctgcag acagcagagc atccagttca cccagcccgt
      481 cactcccatg tacgtgaacg gccgtttcaa gggcttcttc caagggccgc gcggcgccct
      541 gtcccaccag caagccaggc ggagggttga gaagattcaa gaggcgaaac agattcttcg
      601 tgccctgtcc cactggcaca ttgccgcaga cagtagtgat tcccaccacg tccaactcag
      661 gcgaggccaa ggccaggaga atggccaggg cgtcatcgac gccggggtcg gtatcaataa
      721 tgactttggt tggagaagtc ttctgcatgc ctgaatgata cgacgtcggg agagaagagg
      781 gagggatcca ccttcgtccc gacacatgca cggatcactg cgtcttacaa gccggcgtca
      841 cgcctcggag tggactggac gaagcgtgtc gattggccaa gctgttccag tacggactgg
      901 aggacggaca tgttgtgggg cggccccatg aaaaaataaa tcaccgccgt attgtccgct
      961 cccatgaccg ccaacgcccg gccggccatt ccttctccct cgtacgattg cgcgatccat
     1021 gcgtcggttt ctgcttgcgc gcccgctggg ataaaggaga gtccatgcaa cagcaacagc
     1081 ggctcacgaa gttgttcacg aagggcgtct cgtgtgacct tggccagcgg aaacatcatg
     1141 cccagttcct tgccttcctc tggaatgaga aacggcatct ctgcatcgtc aggccgattg
     1201 ctctgccaac cttcgggcac cacaaatgac cagccggtcc caggaattct caatcgtgtg
     1261 cccgcacgat aggtgagtcc ggataccacg tcgagcggac tatcctcgat atgagtgtcc
     1321 gcaacagcaa cggattcaac ggtggcgtgg acgggcaggg cgaacatatt gagcagtaga
     1381 caacctatcg cgatctgacg gacgacacgc cgcacacgac ccacagcggt caaccacgtg
     1441 ccgccgccag aagcagaatc acgagacggc atccaccggc ggacaggtgc aaaagaggtg
     1501 gcggtctcca taggcttcat cgatccggcc cacactcggc caaaacttgt tctcgcgcac
     1561 ccatgaagca ggaaatgccg cttcttcacg ggagtagggt cttgtccatt ccgaggcagt
     1621 cacggccatt gccgtgtgag gagcattctt cagcacattc cccgcgcgcg gctgccgacc
     1681 ttccgcaatg gcctgaatct ccccacggat cgcgatcaac gcgtcgcaca accgatccag
     1741 ctcacttttc acttcactct cggtcggctc aatcatcaag gtgccagcca cggggaatga
     1801 cacggtgggt gcatggaatc cgtaatccat gaggcgcttg gccacatcca tggcttccac
     1861 gccgctgctg tccttgaggg gacgtaaatc cagaataaat tcatgcgcca cgaatccgcg
     1921 tgccccgctg tacaagaccg gatagaactt ctccaggcgt ttggccatgt agttggcatt
     1981 gaggatggcc acctgcgtcg ctttggtcag cccctcgcgt cccatcaacg caatatagac
     2041 ccatgaaatc gtcaggatgc tgggactgcc gtaaggagcg gctgagaccg gcccgattga
     2101 ctgcggtccg cccaatttcg tgaccggatg tccgggcatg aacggcatta aatggcgggc
     2161 gacaccaatg ggtcccattc caggaccacc gccgccgtga ggaatacaaa acgtcttatg
     2221 caagttcaga tgacagacat cagcacccag atcggccggc cggcagagac cgacttgcgc
     2281 attcatgttc gcgccgtcca tgtacacctg tccgccatgg gtatgcacga tctggcacat
     2341 acggcgaatg ccttcctcga acacgccgtg agtcgatgga taggtcacca tcagcgccgc
     2401 caagcggcta cgatgctgcg tggccttggc ctccagatcc gcgagatcaa cattgccgcg
     2461 ctgatcacag gcaacgggaa cgacggtcat gccgcacatc gatgcgctgg ccggattggt
     2521 gccatgcgcg gaaaccggaa tcaaacagat atcgcgctgc gtctcccctc gctgccggtg
     2581 ataggcccgg atcaccatca atccggcgta ttcgccctgt gacccggcat tcggctgcaa
     2641 cgaaatcccg gcgaatccgg tgatctccgc caaccacgac tcaagctgtt ggaacaaggc
     2701 caggtatccc tggacctgat cggcgggagc aaacgggtgg agccgcccga actccggcca
     2761 cgtgacgggt aacatttccg ccgtggcatt cagcttcatc gtgcaggaac caaggggaat
     2821 catcgaatgc accagggaga gatcccggga ttgcagacga tggatatacc gcagcaactc
     2881 atgttccgcg tgataccggt gaaacaccgg atgcgtaaga tactggctgg tgcgagcaag
     2941 cccggccgga taccgcccgt cgaccgattc aagaatggtc tggagttctt ccggggaaat
     3001 ctcctggccg gcgaagagcg tgagcaactg ccgcacttca tccaccgtgc tccattcgtc
     3061 cagcgacagt cccaggctgt gatcatcgta tcggcggaga ttaatcttct gctgccgcgc
     3121 ccggttcaca atcgtctccg attgtgccgg cgagagcggg acccggaggg tatcgaagac
     3181 cggctccagt ccgaccgcgc aaccatgccg gcgcagccct tcggccaagg ccatcgtgag
     3241 accgtgaatc cgttccgcaa tgcgccgtag tccggcaggc ccgtgataga ccgcatacat
     3301 cccagccata actgccaata acacttgcgc cgtgcagata ttgctcgtcg ccttctcccg
     3361 acggatatgc tgttctctgg tttgcaaaga taaccggtag gccgggcgcc cggtgacatc
     3421 tttcgacaca ccgatgatcc gacccggcat ctgccgtcga aactcttctt tggtggccat
     3481 gaaggccgca tgcggcccac cgaatcccaa gggcacaccg aaccgctgac tggaccccac
     3541 cgcgacatcc gcgccgaact ctcccggagg acgcagcagc gtcaaggcca gcagatcagt
     3601 cgcgacgacc acatagactc cggcggcatg ggcgcgcgta atgaattcgc tgtaatcgcc
     3661 cacgtacccg tccgtcgagg gatattggag caacatcccg aagaattcgc ctgaggacaa
     3721 atccaacgat tgaatcgcac cgacctgcag gacgatgccc aagggttccg cgcgggtctg
     3781 caccaccgca atcgtttgcg gatgacaggt ctcagacaca aaaaatttgt gccggtcgtg
     3841 accggccgcg cgggaaatcg cggcgcacat cgtcatggct tcggctgccg cagtcgcctc
     3901 atccagcaga gaggcgtttg caaggggcaa cccggtcaaa tccgccacca tggtctggaa
     3961 attgaccagc gcctcgaggc ggccttgggc gatctcggcc tgatagggcg tgtattgcgt
     4021 gtaccacccc gggccttcga ggatattgcg ctgaatgacc ggcggcgtga tgcaatcgta
     4081 gtagcccatc ccgatcaagg accgccagac ctgattctgc ccggccattc cgcgtaattc
     4141 cgccaagacc tcctgctcgc cgcgcgggga tggaaccgcc agggagccct gcaaccggat
     4201 atcggaagga accgtggcct caacgagaga gtccagcgag ggcacgttta ctgtttccaa
     4261 catcgtctgg atttcagact cagtcggacc gatatggcgg ggcacaaacg tatcgttcgg
     4321 ctcgagaaac tgcggttcag gcatgggggt gaagtcctgc tataaggtga cgcactcgtt
     4381 cattcgaggc agagtgggta ccctatcatg cagcaccgag ttttccaagc gttcagcggc
     4441 catctcacct cgcttgtttt cccaatttcc atagcgtaat agtgagatcg accttgcgcc
     4501 acaggcaacg ttgaccgtca tcagacgcga ccaagacaag aagacttcat catgacccat
     4561 gacattctcg tggttggagc aggactcgcc ggcatgcggg ccgctattgc cgtgccgccg
     4621 gacctcaatg tcgcgctgct ctccaaggtg catccgatcc gcagccactc cgtcgccgcc
     4681 caggggggaa tcaacgcggc catcggcgca gaggactcct gggaggccca tgcctatgac
     4741 accgccaagg gagggctgta tctgggcgac caggacgcca ttgaggccat gtgcaaagaa
     4801 gcgccgcagg acattctgga attggagcgc atgggcgtca tcttcagccg caccgcggaa
     4861 gggcgtattg cgcagcgacc gttcggagga gccggctatc cccggacctg ttacgcagcg
     4921 gaccgcacgg gccatgccct cttacacgcc atgtacgaac agctgatgaa gcgccgctgc
     4981 aaagtgtatg aggaatggta tgtcacggcc ttgctggtcg aagagggccg ctgttgcggc
     5041 gtagtcgcct gggacatgat ccatggtggg ttacagatgc tccgcgcgaa ggccgtgatt
     5101 ctggctacgg gcgggagtgg ccgcattttt tccacgagta cgaacgccgt catcaatacc
     5161 ggagatggca tggcgctggc gtatcgcgta ggaattgcgc tcgaggacat ggaattcgtc
     5221 cagtttcatc cgaccacctt aaaagacacc ggcatcctga tcaccgaagg cgcgcgcggg
     5281 gaaggcggat atctcctgaa cacgttgggc gagcggttca tgaaacggta cgcgccggaa
     5341 caaatggagt tggccacccg ctccaccgtg tccctcgcca tcggacagga aatccaggaa
     5401 gggcgaggtg tcgacggctg cgtcctgctc gacttgcgac atcttggccg cgccaaaatt
     5461 ctggagcggc tcccgcagat ccgggaactg gcgatggaat tcgcaggtct cgatccgatc
     5521 gaaacaccca ttcccatacg tcccggggcc cactaccaga tgggcggcgt ccggaccaat
     5581 gcgtggggag aaaccgagct gccaggactc ttcgccgcgg gcgaatgcgc ctgcgtcagt
     5641 gtgcatggtg ccaaccgcct cggaggcaat tccttactgg aaaccattgt ctttggacgc
     5701 cggacaggca caaaggccgc tgagtccatc cgcaccgttc agctgtcacc ggtctccgaa
     5761 accatcctga agagcgagca acagcgcatc gataagctgc tcagcaaccc cggacctgag
     5821 cgggcctggc agatacggga cgacctcggc aaaaccatga gcctgaatct gggaatcttc
     5881 cgcacgaaac aatccattca agaggctctc agtgccgttc aagcgttgaa acgtcgcgcg
     5941 gaagacatgt gcgtacagga caagggacgc gtcttcaaca ccgacctgat tcacgccctg
     6001 gaattgcagt gtctgctgga gatcgctgag accatcgttg tgggcgcttt aggacgggaa
     6061 gaaagccgcg gggctcacta tcgaggcgat ttcccaaccc gcaacgatac ggcctggctt
     6121 cgccattcgt taagttaccg ccgacccgag ggtccccatc tggcctatgc gcctgtgacc
     6181 attacgcgtt tccccccagc ctaagtcgtc aatcctggga cccgacccct gcattccgtt
     6241 cagcatcgga caacagcctg cccccagcca tcccggcggt tgcgctcctc ccgttttgcg
     6301 ctatcctttt cagtaatcac cgatcactcc actgacggga ccgtccatca cgcgcccggg
     6361 cgaagggtcg taaggcacca tgactaccgt tcaccctggc acggcactcc ctgcatcccc
     6421 tacgctgacc gcgtccgatg cggcacagac tgccatctgt cccttctgtc aggcaatcac
     6481 ctcctgcgca ccagtcgccg gtcatcgttt gcaccagttt ccctgccctt cctgccatgc
     6541 caaccttctg gtcctcaacc cggcggcacg gccgaccgat gaagacaaga agactcgatc
     6601 gtcggacggc gatcttccaa tcgatctttc acaccggctc acaggcatcg cagtggctgt
     6661 cctggcctgt gcgttgaact acgtgttgtt gatgggcatg gcctactgga tcctggggca
     6721 agtaaaaacg acccacgatc cctacgacat cgatgcgttg atgttgtcgc cacttatttt
     6781 gaaagccaat ggctggacgc ccctccacct tgcggcggcg cgaggcgatc agtctgcggt
     6841 cgcaacgctc ctcacagaag gacaaacggt ggaccaacgc aacggcaatg ggcggacggc
     6901 attgtttgaa gccgccaagc gcgggcagac cgatgtggtc acgctcctgc tgcaacatgg
     6961 agcgaatccc agcgcgaagg gcaaacaggg ctttactccg ctgttggctg cggcagagca
     7021 gggccatgcc gacaccattg aggtgctcct taccaatgga gccgacatcc gagccacctg
     7081 cacctgtggt gattcggcgc tccatcgggc ggtccgacat gggcatctcg ccgccgcgca
     7141 gacgctcctc gaagggggca ttccggtgaa ccagaagagt cacggcgaga cccccttgga
     7201 aatcgcacaa cacgacgaag atcacgaact gatcgcgctg ctgcgcgcat acggcggtag
     7261 agagttttct gacgccaagg ccaaacgcgc ggaaggaatc gcattccaaa aacacggaca
     7321 gatcgaccac gcgctcttcg cctatgctga agcattgaac ctggatccgg atgatcatga
     7381 agcctattac ggacgcggaa ccgccctgct ccaaaaaaac gcgcctgacg aagcgctcat
     7441 cgcctttcac gccgccattc gtctgaatcc tacctacctc gatgcctaca gcgcagccgc
     7501 gtccgtgcat acttcacgcg ggcaatggga tcaagccctc aggctgtggg atcagctcct
     7561 ctcacaccag ccccaaaatg gacgtgccat tttcgaacga tccatcgtga aacgaggaaa
     7621 aggcgatagc gcgggattcc tgcaaggatt gcaacaggcc tgcgccttgg gccatcgggc
     7681 cgcctgttga atcatgagca atgcggcact gcatgacgac acgagcacgg cacgcataac
     7741 ctgtcaccgc tgcctcagcc attaccgagt ccgcgccctg gaacacttga cgggggcctc
     7801 tcgcagcacc tgccccacgt gcggcgcccg gttcgcggtg gtcgacccgg tatcgtcagc
     7861 ctctgccgaa gtcctgccga cccggcaaga caggccttcc gcggttcatg actccgcgtt
     7921 accaaccgac gcggctgtat ccatgcctgc ccctatgcgc gcgtcctttc acggaatggg
     7981 cgggaccctc ctgggcatgc atgtggtgaa cgtctgcctc acgctggtga cgctgggcgg
     8041 ctatcatttc tgggccaagg cgaagatccg tcggtacctg ttcagccaca cagcctttgc
     8101 aggggatcgt ttcgcgtatc acggcaccgg caaggaactc tatcaggggt tcctgaaggc
     8161 catgctggtt ttcggtattc cctatttttc tctaagcgcc gcgcatacct tcctggagct
     8221 tccgcagtgg atcgatgtct tgctacaggt gatggccggc ctggtgttct tcctctatgt
     8281 ccccgtcgcg atcgtgaatg cccgccgcta tcgatgcacc cgcacctcat ggcgcggcat
     8341 ccggttctcg tttcgaggac ggaccagcga ttttctcaaa ctgtattttc ggggatggtt
     8401 ctttacgatc ctcaccctgg gcacctacta tccctatttc caaacgcagc gtcaggcgtt
     8461 tctgcactcc cacacctatt tcggtaacca gcgtttccgg ttcaccggcc atggatcagg
     8521 agtgatggtc cccttcgcca tcaccctatt taccacctat gccgtcctgg ccctatgcgg
     8581 gttcgccctc gccgcgcaat tgacgaacgc gggactgacg ctgttgttga tcccctgtgt
     8641 gctcggaccg atgtggatct ggctgctggg acagaagcag cgatacttct ggaatcacac
     8701 ctcattcgga gacgcccagt tctcatccga gattacctgg caacggctgt tcacgctgta
     8761 tctgggcaac ctcggcctcc tcctggccac cttgggatgg gcctggccct gggtcacggt
     8821 gcggaatgcc cgatttttta tgggtacgct gtcgctccaa ggccccgtcg atctcgatcg
     8881 cgtattgcaa gacacctccg aagcctcggt caccggcgaa gggttatcaa acctcctcga
     8941 caccggcttc gacatggatg cgtgatcatg tccaccgccc gaaccgccca ctacctcgac
     9001 ggccgcacgg ccacgcgaca ccgtgtcacc ctcaccataa gcccggccgc gctgcagatc
     9061 gccatgcccg acggcagcac gaaggaatgg ccgtacggcc agattcgcca gacacaaggg
     9121 acgtaccggg gcgagccggt ccgcttggag ttcgggactg aaccggcaga ggcggtggtg
     9181 gtcgccagcc gggcgctctt gaccgatatc cacacggcag cgccagcact cgcacaacat
     9241 tttcacgatc cctcctggcg acagagacgc cttgcctgga cgctgggcgc gggactcggg
     9301 gtgatagtca tgctcatcgg cctgtaccga tggggcattc ctggtatcgc ctcagcagcc
     9361 acgccctacg taccgatcat gtgggaagaa tctcttggcc ggcagattgt ccaacacctc
     9421 gcgccggaaa cgcagcaatg tcgagagccg gagcgacttc gaaagatcga ccaggtggtg
     9481 caaacgttgg ccgccacaca tccggactct ccgtatcgga tcacgctctc cgtggtagac
     9541 aatcccgcca tcaatgcctt tgccgctccc ggcggccagg tggtaattct gcgcggcctg
     9601 ctggaacaga ccaccagcgc cgagcagctg gccggagtcc tggcacacga actccagcat
     9661 gtctatctcc gtcacagcac cagagccatc cttgaacaga cagccggcac gctgctgctg
     9721 accgccgtat cgggagatct gtcaggggga ctggcctggg ggctgcaagg tgcgcagacg
     9781 atgggatcat tgcattacag ccgcacccat gaggaagaag cggatgtcga aggccttcgt
     9841 atgatgcagg ccgcccatct cgatccgacc gccatgattg ccttttacgg gacgctgcag
     9901 aagtcggagc aagaccatat cgaaccgccg gattttctct cgacccatcc cgatatgagc
     9961 cggcggctcg ccacgcttct cgcgcttgcc ggcccgcccc cgaccgacgc gcgccagttg
    10021 cttcccggag aagattggaa ggacattcgt accctctgtc gcctacaaac cgccacgcgc
    10081 tccagttcag actcccccga ccttccctga ctggcacgtt tgatcgcatc atcggccgtc
    10141 cgcctctgct ataatccacc cacctgtgga catgacctat gcgcgtgctt gtcgttgaag
    10201 atgaaaccaa ggtaggctcg ttcatcaagc gagcccttga ggaagaaagt tacgccgtcg
    10261 acctctgtga ggacggcgcg cagggcctcg atctggccct gaccggcagc tacgacctca
    10321 tcatgatcga cctgatgctg cctggcctcc ccggtatgga agtgctgacc cgccttcgca
    10381 aagaaaaaat tcagacgccc gtgttgatcc tgaccgcaca gtcgaaagtc gaccagcgcg
    10441 tcaaaggact ggatgccggg gcggatgact acctcaccaa acccttcgcc atcgatgaat
    10501 tgctcgcccg tgtcagggca ctcctgcggc gcggcaccgc ggaatcgtcc ggcacactcc
    10561 agatcgatga tctcatcctt aacccggcga cgcgggaagt cacccgcgga ggacaacgta
    10621 tcgatctcac ggttaaagaa tatgcccttc tggaatactt catgcggcac gcaggacggg
    10681 tgctgacccg ccccatgatt tccgaccatg tctggaatca agacttcgac acattcacca
    10741 atgtgatcga cgtctacgtg aattatctgc ggaataagat cgatcgcggg cgggcgcgaa
    10801 agctgattca tacgatacga gggagtgggt atatgctcaa ggtggactga tgccccttcg
    10861 agtccggctc accctctggt acggaacggc gctggccctg atcctgctga cgttctccgt
    10921 ggtgctctat accatcacgg cccgcgggct ccgcgaccag gtcgatgagt ctttgcagga
    10981 aaccgcctca gccgccgtgc gttcactgga gacccggggg ttccttcccc tcatcgatga
    11041 agatgcgctg ttgagtcaat ttcccgaact cactcgtatc gataaatttt ttcagatctt
    11101 cagccccacc ggcaccatca cgatccgctc tccgaatatc cgacagcacg atgtcccctt
    11161 aagccgaccg gccctggaag cggcctttac cggcagcacc gtcttcgaat cggccagata
    11221 tcccaatgaa ccgccgctgc gcctggtgtc cgttcccatt atttaccgtg gctcattgct
    11281 ctatatcgtg caggtgggaa cgacgctgga aggcgtcgaa gaaacgctgc gccgctttct
    11341 cctcgtgctg ctcgtcatgg cgccgatcgc cttggtcgtt tctttggtgg gcggatggtt
    11401 tctggcggga cgtgccttgc gcccggttga ttccatcacc gtcgccgcgc aacggattgc
    11461 ggccggcgac ctgacgcagc gactgcattc tgaacgctca acggatgaaa tcggccgtct
    11521 caccgatacg ttcaacaata tgatcgcccg gctggaaacc tccttcgcgc aaatccgcca
    11581 gttcagcagt gatgcctccc acgaactccg cacgccgctc acggtcatga aaggtgagag
    11641 cgaattagtc ctgcgccgtc cccgaccggt cgaagactac atcgccgtgc tcgaaagcaa
    11701 cctggaggaa atcgatcgca tgtcccgcat cgtcgaggaa ttgctgttcc tctcgcgggc
    11761 ggatatgggc caggtgaaaa cggaatgcga gccggtccgg ctggaggcgt tagtggagga
    11821 cattcaacgc caggcctgcc tgcttggtca ggaacgggaa gtggatgtca tcatgggtac
    11881 gattcagccg gcgaccgtgc ggggagacga acttcgtctg cgggaactca tcctcaacat
    11941 cgtcgacaat gcggtcaaat attctcaccc ctgcggcaag gtcgaaatcg atttacgggt
    12001 agaaggctcc accgcgcgcc tggccgtacg cgattacggg atcggcattc cgcaggacgc
    12061 gcacaagcag attttcgacc gcttcttccg cacggatgac gcccgggccc ataccaaaaa
    12121 agggaccggc ctaggcttgg ccatctgcgc ctggattgcc gaggcccacc acggacacat
    12181 cgaagtccaa agcgaggtcg gcaaaggatc cacattcact ctcgtcctgc cgttggcggc
    12241 cacagcagct taacgacttc taattcctcg ttaatagttt tctcatccag ggctgttacc
    12301 ttccaattga ctgtcacacg gaagattcat ctctcgcgtg tacacatcac cgtcatctag
    12361 gaggagccca tatgaaacga cccacaccgt cagtcagtgt cttagccggt attgccgctg
    12421 tgagcgcggg gttaatctgg agctatactc cgctttcacc gtctcacgcc tccaatccat
    12481 cgcccgtcgt agaggaacgc ccgatagcgc cgactggcgc cttgcccgct gtcggattcg
    12541 ccgatgtggc caagacggtc acaccagccg tcgtcaacat caccaccagc ggcgcggagg
    12601 aagtctctga ccacggtcga ccacggggga aaatggacga cttcttcgga tcaccgttcg
    12661 gcccgcgtcg attcggcccc cccacggaac cgcgggaaca tcgaggtggt ggccaggggt
    12721 ccggggtgat cgtcactcag gatggatatg tgttgaccaa taaccatgtg attgcaggag
    12781 caaaaaccgt cacggtcacg ctacccgaca aacgcgaatt taaggggcgc atcgtcggca
    12841 gtgatccaaa gacagacatt gccgtggtca agatcgacgg cactcagctt ccgactgttc
    12901 cctggggtga ttccagccgt ctgcaagtcg gtgaatacgt actggcggtt ggaaatccct
    12961 ttggcctgaa ctcgacagtg accctgggca tcgtcagtgc gctgggccgt ggacatatgg
    13021 ggatcacgca gtatgaagat ttcattcaaa ccgatgccgc catcaatccc ggaaatagcg
    13081 gcggggccct cgtgaacacg aagggagaac tgatcggcat caatacggcc atcttttccc
    13141 agaccggcgg atatcaaggc gttgggtttg ccgtctcgac cggaatgagc aagccgattt
    13201 atgaaagcct ggtcaagacc ggcaaagtcg tgcgcggcta cctgggcatc ggcattcaag
    13261 atctgaacca tgacctcgcc aaatcctttg gcgtgaaggg aacgaatggt gcagtcgtca
    13321 ccgatgtgaa ggaagaaggt ccggcggata aggccggaat caagcagggc gatgtgatcc
    13381 tttccttcca gggtacgccg atcgaggatg ccgtgacatt gcaacgcgcc gtgacacgaa
    13441 ctgcggtcgg cagcaaagcc acggttacag tattgcggga cggccaggaa aaagagttgg
    13501 ccgtgacgct ggccgaactg ccggagaacc agcaggtcgc gaagacagag actgaaccct
    13561 cggatcagcc gctggcgggc ctcgccgtgc aggaactgga tcgggaaacc gcccaggaac
    13621 tcgggttgaa gggaaagatc caaggagtgg tggtcaccac ggtcgatccg gagagtgatg
    13681 cagagcgtgc cggcttgatg cccggtgacg tcatccggga aatcaaccgc aaacccgtca
    13741 cctccatgaa ggagttcgat cgggccgcat cgagtctcaa gaagggacaa accgttctgg
    13801 tcttgatcaa ccggcgcggc gcatccctgt atctgagcgc gaagatttaa ctgcgggcag
    13861 aacgacgggg cggggtcccg gtgagcacgg gcttatcggt aacccgcccc ggcttttttg
    13921 ggcatgagca aaccaggaga atcggaagaa cggtttggct ggtgggtctg accagcaggc
    13981 tgggcaatca gatcataccc ggcgacatct gtcaccgtga ccgaaatgaa ttcgcccggc
    14041 ctggcaatcc cacgctcaca atacaccacc ccgtcgatct caggggccaa gccctggtgc
    14101 ctcgcctcta gcagccggtc cgactcttca gacaccccat ccaccaggac ttcgatcgta
    14161 cggccgagat actcacggtt tttggactct gagatcgatt cctgcagggc caggagctca
    14221 ttgcgccgtt cttccatcac cgcctggtcg acttttcggt cgagatccac ggccgatgtg
    14281 ccttcctcgt cggaataaag aaatacggcg acacgatcga actccatgtc gagcacgaac
    14341 tgcttcagct cttcaaactg cttgtccgtc tcacccggaa acccgacgat aaacgccgtt
    14401 cgaaaaaaca cgccaggaat tttggtccga atacgctcca ccaacttggt cagatgcttg
    14461 cgatcaccca gtcgatgcat gcgcttgagc atcccgtcac tgatgtgttg cagcggcata
    14521 tcgagatact tcgtaatgcg cgcctcgccg gcatagagct ccagtaactc atccgtgacc
    14581 tgctggggat ataaatagaa cggccggatc catcgcacat cgtccaccgt gaccaattca
    14641 cgtagcaacg ccgtgagtcc ctgtttgagc ccgagatcca ccccgtaatt gatggtgtcc
    14701 tgggaaatga ggttgagctc cttgacccct tctttggcca gccgtctcgc ttcagccacg
    14761 atcgagtcaa tcggtcggct ccgttgcttg ccccgcatga tgggaatggc acagaaggcg
    14821 cagttgcggt tacagccttc cgcaattttg agataggcgc tgtggggcgt ccccagacgc
    14881 acgcgcggcg tgtcggcgtc gtagagatac ggcggctgcc ccagccagag acgttgctgg
    14941 cgttttttcg gagccaacag gctgcggcaa atatctgcga tgcggccgaa ttcgcccgtt
    15001 ccaaccacgc cgtccaactc gggcagttcc ttcaaaagct cgccctgata acgctgcgcc
    15061 agacagccgg cggcgatcaa gacccggcag gagccggact tcttgagacg gccatgctcg
    15121 ataatgctat tgatcgactc ctgcttggcc tcttcgataa agccgcaggt attgataatc
    15181 acgacttcgg cggcacgggc atcgccggtc agttggaatc ccccggccac cagcgttccg
    15241 agcatgactt cggagtccac ctggtttttg gaacaaccca ggttcacgaa gccgatggtg
    15301 ggtttcttac ctttcgcccg tgaaggcgta atcaatgatg aagccataac gaggcagtct
    15361 acgagacagc ggaaaagtct gtcaaacgag ctctcttgct attcgtgcta cagctatcct
    15421 agattgagcg gatgattcgg accttccagg gcatcaagcc cacgatcccc caatcctgct
    15481 ttatcgaaga aacggcggtc atcatcggcg acgtcgtgat gggcgaagag tgcagcgcct
    15541 ggttccatgc cgttatccgc ggggatgtga actacattcg tatcgggaac c
//
LOCUS       DAOQEW010000082        15576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1045_length_15576_cov_8.128922, whole genome shotgun sequence.
ACCESSION   DAOQEW010000082 DAOQEW010000000
VERSION     DAOQEW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1045_length_15576_cov_8.128922"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2194..2430
                     /locus_tag="PLS35_10260"
     CDS             2194..2430
                     /locus_tag="PLS35_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19092.1"
                     /translation="MSKHKRRDKHRPGSMDEMIVNAIDQGLLRSAATAGQLVMEQVLK
                     TDPEAKSLLETLVKQRLKQVLRNLDESGKANGKN"
     gene            complement(2488..3201)
                     /locus_tag="PLS35_10265"
     CDS             complement(2488..3201)
                     /locus_tag="PLS35_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNI19093.1"
                     /translation="MMRPPRRQSKAALVFLFDVDETLLDDQRVLSDLRRHLRREVGRE
                     GDRRYWNILQRLHRALGYADYLGALQKYRQSYPHDPHVLTLSSFLINYPFAKRVFPQA
                     MRVITRCRRVGSVVILSDGDVVFQPHKIQQSGLAAAADDHVLVYVHKEEQIRNIEVCY
                     PADHYVVVDDNVRILNVIKKIWRERVTTVWVRPSSSGTGRNGIHAHAHPDLTIHSIGE
                     LNKEHLRAIKLLQKEQSHS"
     gene            3557..3973
                     /locus_tag="PLS35_10270"
     CDS             3557..3973
                     /locus_tag="PLS35_10270"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNI19094.1"
                     /translation="MFCLVYASSATRPFSPSQLTDLLASCHANNSRLGITGILLYKNG
                     NFMQALEGEEEAVLKLYAKIFQDERHSGVLTLLQQTIPARQFPDWSMGFRDLNSPEVR
                     SLPGYNEFLNMPLTGAEFSSDPTYCQRLLTIFKKMM"
     gene            complement(4100..4690)
                     /locus_tag="PLS35_10275"
     CDS             complement(4100..4690)
                     /locus_tag="PLS35_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19095.1"
                     /translation="MIRLARKPDGMFSDLLDSAITQIVNDYLTSANARDQEDWLCASM
                     PRAARFFTAPQAREQLLAMQKIYHDERLYEVTKYHWLLLYECLETFAQGFNEQPVGWL
                     AQKYGIEKIDFHRLVELFFWDTSFLMDHLAKMSRQETRTMLIWPESVGLAAGFKTHPD
                     ELAFILCDEGVTEEFAAQKDVPAWSIGSKSYPLPHT"
     gene            complement(4944..5345)
                     /locus_tag="PLS35_10280"
     CDS             complement(4944..5345)
                     /locus_tag="PLS35_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19096.1"
                     /translation="MLTPQRPLKLLIIDDSPDFQLLIKTYLEDFGIVCLFATDVVQAT
                     STAVREQPNLILLDIGLPGGGGLLLLERLRANLRTAKVPVIVITAQTTDGLESKARSL
                     GAEEFLHKPVEKDVLIDTLRRVLSRPPTQQA"
     gene            5992..6396
                     /locus_tag="PLS35_10285"
     CDS             5992..6396
                     /locus_tag="PLS35_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNI19097.1"
                     /translation="MAVRNVAIFILYTSSGQLLLQHRTPDAYRLPNYWAFFGGGIEPG
                     ESPSDALKREVIEELSYHVQDPHFLMTQLFREGEDENTKYVFVERYQGQPLELGEGQA
                     MGWFLPDETHDLKMIDHDRAIVERVRQYLRDR"
     gene            6664..7521
                     /locus_tag="PLS35_10290"
     CDS             6664..7521
                     /locus_tag="PLS35_10290"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNI19098.1"
                     /translation="MPRRTRAASPTRPARAKDAVLDYPGYALKSGILAPSTIRAVQQR
                     LNQVGCGPISVSGVFDRATQEAVRLLQARSVDGQGQSLKIDGVVGPLTWAALFGAHRV
                     PSIATTPISALMKATLSIAASQVGVMEDPSGSNRGPKVDEYLKAAGIDPSTGSYAWCA
                     AFVYWSFKQAATRLGTTNPAIRTAGVLDHWKKAGQAGVTRLVAGQILDDLSLLKPGLV
                     FVINTGGGHGHMGLVEDFRDDRLVTIEGNTNLPGDREGVGVFRRSGRTISDINQGFLS
                     YDSQSHASS"
     gene            7716..9101
                     /locus_tag="PLS35_10295"
     CDS             7716..9101
                     /locus_tag="PLS35_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNI19099.1"
                     /translation="MQDIDSNPSFTLTDVPGGLPWLGHLRSFKHQPLRTMSGWWRQQG
                     DAVRFRLGPKTIHLFSHPALAEEVLLSQAERFGKVYEQQRPTGLALVLGNGLVTSSGE
                     VWKRHRRIIQPVFHRSRMAAMADRMVQVGEQRLAGWATHGEQPVDIAAEMMQLTLEVI
                     SQTMFHTSVAQHMDQISHSLRVSLKYAFDSFHNPLHLPVWVPTARNREFSRALKFMDT
                     LIYELVAARRQSGAHYDDLLNLLLQARDEETGMGLTDQELRDEALTIFAAGHETTANA
                     LAWTWYLLATHPEVRTRFHTEVDLVLRGRTPTADDLQHLPYTRAIFDESLRLYPPVPA
                     IQRKSMTQTTLGGFTLPANAIVLVGLYNLHRHPAFWADPEQFLPERWLDGERPPSRCA
                     YLPFGVGPRACVGTHFATVEGPLLLALIGQRYDPQLAQDHVEPEVMVTLRPKNGIRMT
                     LQQRHQSVGSE"
     gene            complement(9202..9278)
                     /locus_tag="PLS35_10300"
     tRNA            complement(9202..9278)
                     /locus_tag="PLS35_10300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9242..9244),aa:Pro,seq:tgg)
     gene            complement(9269..9880)
                     /locus_tag="PLS35_10305"
     CDS             complement(9269..9880)
                     /locus_tag="PLS35_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNI19100.1"
                     /translation="MQIVLATRNQHKKQELVALLGGLDITIRTLDDFPEAPEVVEDGE
                     TCEANAMKKAVEISRFTGLPAVADDTGLEVDALGGRPGAFAARYAGEHATYEDNCRKL
                     LQELSGIPPARRGARFVTVAAIAFPGGKTMSAKGLLEGVIAESVVGSNGFGYDPVFIL
                     PEYNQTLGQLPPEVKNRISHRARAFAQARTLLQQAMAEHSVGA"
     gene            complement(9883..10626)
                     /gene="rph"
                     /locus_tag="PLS35_10310"
     CDS             complement(9883..10626)
                     /gene="rph"
                     /locus_tag="PLS35_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNI19101.1"
                     /translation="MTSGAGLGRIDGRRRDQIRPVKVTRNFTKHAEGSVLIEMGDTKV
                     ICTASVEEKVPPFLKGKGTGWVTAEYAMLPRATHDRSPRESVKGKQGGRTLEIQRLVG
                     RALRAVIDTGRLGERTIWIDCDVIQADGGTRTASITGSFIALADAITVLKKKDLLKVN
                     PLTDYLAAISIGKVGGQVMVDLAYEEDSHAEVDLNLVMTGAGQYVEVQGTAERTPFNK
                     KDMDEFLDLGWGAIRELVDLQKSLIGALS"
     gene            11080..12258
                     /locus_tag="PLS35_10315"
     CDS             11080..12258
                     /locus_tag="PLS35_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19102.1"
                     /translation="MSIPSVSEVTTQQSILENRHILIVDDEEPIRRLLGYLLEPHGYQ
                     VTLAGEAREARQRMEDGYYAMVLCDVNMPGESGMDLIRHILTQYPNTAVIMITGLDSP
                     VLANAALDMGAFGYIIKPFEANEVLINVANALRRRKLEIENAMHRENLEEVVRTRTIA
                     LQQALEWLERSEKELRLSREETIQRLAIAAEYRDSSTAQHIQRMSHYCELLARRYGLS
                     PDRCDLIRTASPMHDIGKIGTPDHVLLKPGKFTPEEFKVITQHTEIGYRILAGSDSDL
                     LKVAALIAWTHHERYDGTGYPRGIKGEEIPLEGRIVAIADNFDALTTQRVYKPAFDFD
                     HAKELMLKERGKHFDPDLLDIFFESMADVKRIFDQFADPSWLSSSRHRAITHDQGEAV
                     "
     gene            12255..12752
                     /locus_tag="PLS35_10320"
     CDS             12255..12752
                     /locus_tag="PLS35_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19103.1"
                     /translation="MKRTSRNWIAYLCENLASSGMMPTWEAATYLTDNLFGDKPNPRL
                     LRTTCPYEDTTHLLQRLYQPLVAAATRDMPLPAAAMIHVFTDISLTGKSAVLVVYFPG
                     PTQPHQLLLLDAAKAWELVFENSDEFNTWAEARYHSIKAALAKILSGVAPSPTLPASL
                     EAVKN"
     gene            complement(12861..>15576)
                     /gene="metH"
                     /locus_tag="PLS35_10325"
     CDS             complement(12861..>15576)
                     /gene="metH"
                     /locus_tag="PLS35_10325"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNI19104.1"
                     /translation="EAVRGVSPRRPAKVEPFLRLSGLEALTVRPESNFVNVGERTNIT
                     GSPAFSKLILAGDYDKALTVARQQVEGGAQIIDINMDEGLLDSKVAMQKFLRLLAAES
                     DIARVPIMVDSSKWEVIEEGLRNIQGKGIVNSISLKEGETKFLEQARLIRRYGAAIVV
                     MAFDERGQADSVARRIEICERSYRLLTEQVGVPPQDIIFDPNILTVATGLEEHNNYAV
                     DFIEATRWIKAHLPLAKVSGGVSNISFSFRGNNVVREAMHAAFLYHAIQAGLDMGIVN
                     AGQLAVYEEIPKDLLALVEDVLLNRRPDATERLVTFADTVKQKGKAAVKDDEWRQRPV
                     EERLAHALVKGLTDYIDQDVEEARQKSARPLDVIEGALMAGMNIVGDLFGSGKMFLPQ
                     VVKSARVMKKAVAYLMPFMEAEKQRLGTSRANGKVLLATVKGDVHDIGKNIVGVVLGC
                     NNYEVIDLGVMVSCEKILETAREQQVDMVGLSGLITPSLDEMVHVAKEMTRQGFTVPL
                     LIGGATTSKAHTAVKIAPSYANTTVHVLDASRAVGVVGSLINKQQEQEFSASIRADYD
                     RIRQAHQDRGSKALRTVEAARTHRMPTDWASADIPTPSFTGLRQIDRMPLRELVPFID
                     WTPFFHTWELRGRYPEILEDATVGPKAKELLADAQALLEEIIRGELLTARGVYGLFPA
                     NSVGDDIELYRNGDRRDVLTTFHTLRQQTEKPADQFNLALADYVAPKDSGRGDYVGAF
                     VVTAGIGVEALCAKYEKDHDDYNSIMVKALADRLAEAFAEWLHKQVRREWGYGKAETL
                     TNEDMIRERYRGIRPAPGYPACPDHTEKRILFDLLQAESQSGVTLTESYAMLPAAAVS
                     GLYFAHPEAKYFAVGKINRDQVEDYAARKKLSVGEVERWLAPNLNYDA"
BASE COUNT         3520 a         4509 c         4145 g         3402 t
ORIGIN      
        1 ttagaaggct gatgctctat ccgactgagc tacgggcgca cacttatttt tcaatcattt
       61 atgagatgag tatccaagag agctttcgcc tagtgttccc ggcgtgttct cttggaggta
      121 tctgtgaacc ttttgggcag cccgagtcaa gtctcgcgcc tcgatcgtat tgtatcgttt
      181 ccacatcttg tcggacttgt ggccacaatt ttcatggcag tggccgtatc cacccctgct
      241 cttctgagat tagtggaggc gcagtggcgc agatcatgaa accggaaatc ggcaatatca
      301 gccttcgcaa tggcggtcct gaaagaacgg ctgacgcgtt ttatcgcgtc ggcctttata
      361 cggtaatacg tgtctcgtgg caagactgcg tctccgagca agtcgctgca aggtcatttt
      421 gacatcgggt gtcatgggaa cctgacgagc agttttggtc ttggtgtcca gggaacgaag
      481 ggtgataaat cccctcttca catcgacacg atctcaagtg agtccgacaa tttcgctaaa
      541 tcgttgtcca aattgatagg ctgtgagcag agccctcctc agacgggtct tggcctcact
      601 atacaatcgc cctcactcct catcggtcaa tacacgatcc cgttcattgt ggggattcgg
      661 cagaggaatt tttgaagcaa ggatggcagg agttgtacta gtggcaggca aagaataaag
      721 gatttaatgg agtgggtagc ccgggacatg cagatgaaat ccatgtcccg gggcagctcc
      781 tttaacggac gaattaacag ccggtggatt caagccttgc ttttcgcttg cgcctggcca
      841 gcgcggtcac tgcagccaaa ccacttccaa acagccacgc agcggcgggg acgggcactg
      901 gagcaactgt caacgaggtg agttggcccg taactcctcc aactttacct agtggctgaa
      961 caaagcccag gcttatccgt gtgtcgctgc ctgatagact tggcggagtt aacgggaggg
     1021 aatcattggg aaactgtcta aggccagaaa ttgacataga aaatgaagac gggagcagtt
     1081 cattgaccgc aggaccagat ggtcgaaatg agactcgata gctatcatcg gataatgcaa
     1141 agtcagtggg tcttccaaac gagttgctaa tcaccgtgat cagcggagga gtgacagtgg
     1201 gcgccatatt ggcagaatac gtattgcctc ccaaggttac cgaggcgttc gtaagcagat
     1261 aattaccggc ttgcgttgaa ggggttccgt ctggagtgag cgaatcaaag gtatatgaac
     1321 cttggaacgt gttggacgta ctgaacggtg gactcaaaaa cggggaaaca gcgatgacac
     1381 ttccttcaaa ctgaaacgtg attaaagcag agtaactgac tcctatcagt ccaccccacc
     1441 ctaggcatgc ggcaagtata gctacgatcc tgacacgaca ctgatgcaca gaacaattca
     1501 gcattctacc gctccttttc atggtcttga acgatacgtc aagaatgcac aacagcaatc
     1561 ccgccgcggc aatagcagta ctatactccg attatgacaa cgcataaaca cttatatcat
     1621 gaatccctgc tgcatgacat acttcaggca atcaatggca gatgccagag ccaataatga
     1681 tgagcctact acttaggtga gagcccaatc ataagcgatg aggtaagatc gtgacgtata
     1741 gtcactcgca ctgcgaggga gtcacatctc tattcttcaa cgatacggca agatcagcac
     1801 aggcttcact ggggagctgc aacttggatg catgatcacc ggcccaagga tctcacagga
     1861 caactgccaa ccagggatgc tcccagtatt cccaggtctt tgtaccttgc gactatggac
     1921 ctcgatgcct catgagctaa gaagcgctga gatatcattt ggcttcgtca cggtccaagc
     1981 cggcattcat tccgccatcg agcagcaaga tactcacttc gaccgacatt gtgccgctgc
     2041 caagctctga catccagaat ctcagctggg aaaggagtca caaggctgcc ttgtctggaa
     2101 ggagaaccgg cacgttgccc gccttctacg tgtgagtgga aactttttta gcatgaaccg
     2161 cataccttta tcatgagcga cgcaggagag gtcatgagta aacataaacg gcgcgacaaa
     2221 cataggccag gctccatgga tgagatgatc gtcaatgcca tcgaccaggg tttacttcgt
     2281 tcagcagcca cggcaggcca actggtgatg gaacaggtac ttaaaacaga tcctgaagcg
     2341 aagtcactgc tcgaaaccct ggtgaaacaa cggctcaagc aggttttgag aaacctggac
     2401 gaaagcggga aggccaacgg caagaattaa tacgtcgatt ttggggcacc aagtgtgtcg
     2461 aggtgctcag atgtgcaggc cgactcttca agagtgactc tgctctttct gtagcaactt
     2521 gatcgcgcgc aaatgctctt tgtttagttc tccaatagag tgaatggtga ggtcggggtg
     2581 tgcatgcgcg tgaataccat tccgacctgt cccacttgaa cttggcctca cccacacggt
     2641 ggtcacccgt tccctccaga tctttttgat gacgttgagg atacgaacat tatcatcgac
     2701 gacaacatag tgatccgccg gatagcacac ctcaatgttg cgaatttgct cttccttgtg
     2761 cacatacaca aggacatgat cgtccgcagc tgcggcgaga ccggattgtt gaatcttatg
     2821 cggctggaac acgacatccc catcggaaag aatgaccaca gagcccacac gccggcagcg
     2881 tgtaatgaca cgcatggcct gcggaaaaac acgtttcgca aaggggtagt ttatgagaaa
     2941 ggaggatagg gtaaggacat gagggtcatg gggatacgac tgacgatatt tttgcagggc
     3001 acccagatag tccgcatagc ccaatgctcg gtgcaagcgt tgtaaaatgt tccaatatcg
     3061 tcgatctcct tcacgtccga cctccctccg taaatgacgc cgaagatcgg acaacacgcg
     3121 ttgatcatca agcagcgtct cgtctacatc gaacaagaag acaagagccg cttttgactg
     3181 gcgccgtgga ggcctcatca tgcttgtgtc ttacttcaca aaggttaacc agtgggcaca
     3241 agcgacatat tttacacctg cactacctgg catgtgactc tccctgagag cgttccacaa
     3301 aagttatgaa ggatcgagag acgtgagttg gactagcaat gttcctagtt tgtgcgggtt
     3361 gatctgaaga tgactattcc gttgggaccc tcgtcagccc ggagacgcct gaacgtgaga
     3421 gaaggaaagt taaattgact tgatcgagcg caacaacggc agcagagtat ccctgatatc
     3481 acacacatta ttcgtcatat ggcgcagttt ttttacgacg gacgatagca aagatgaccg
     3541 tcctagggag gccataatgt tttgtcttgt gtatgccagt tctgcgacaa gaccattctc
     3601 tccatcacag ctcactgatt tgctcgcctc ttgccatgcg aacaacagtc gattaggtat
     3661 taccggaatc ttgctctaca agaatggaaa ctttatgcag gcccttgagg gagaggaaga
     3721 agcggtgttg aagctctatg caaaaatctt tcaggatgag cgacatagcg gagtgctcac
     3781 actcttgcag caaaccatac ccgcgcgaca atttcctgat tggtccatgg ggtttcgcga
     3841 tctgaattcg cccgaggtca ggtcgctgcc gggatataac gagttcttaa atatgccgct
     3901 caccggagcc gagttctcct ccgatcctac ctactgccaa cggcttctga cgatattcaa
     3961 aaaaatgatg tagtgagtgt ctgtgagtcc tcaatctcct tccctctttc cctccttcca
     4021 catacccatt gaccaatacc aagacatttg gcctcgttgg ttacgatggg atacagaacc
     4081 tgggatgaat acgcgttccc taggtgtgcg gcaaggggta ggactttgac ccgatagacc
     4141 atgcaggaac gtctttttgg gctgcgaact cttctgtaac tccttcgtcg cagaggatga
     4201 acgcgagttc gtccggatga gtcttgaacc cggccgcaag cccgaccgat tccggccaga
     4261 tcaacatcgt ccgtgtttcc tgacggctca ttttggccag atgatccatc agaaacgagg
     4321 tgtcccagaa aaacaattca accaggcgat ggaagtcgat cttttcaatg ccgtattttt
     4381 gagctaacca ccccaccggc tgttcattga atccctgcgc aaaggtttcc agacactcgt
     4441 acaagagtaa ccaatgatat ttggtcacct catacaatcg ttcatcatgg taaatcttct
     4501 gcatggcgag caactgttct ctcgcttgcg gagcagtaaa aaatcgggcc gcgcgaggca
     4561 tcgatgcgca gagccaatcc tcttgatccc gtgcattcgc cgatgtcaga tagtcattga
     4621 cgatctgtgt gatcgcactg tctaacaggt cagagaacat accgtcgggc ttcctcgcca
     4681 ggcgaatcat gaatcgctcc tgtgactctt tgcagatttc tcctcacacc accactgatg
     4741 tctcatttcc atcctgtctc tggtcctcag ccatttctgg atcatccacc gccgtgacga
     4801 caagggacac tattagggac gcaggtctga ggttccagcg tgacgcaccg cacgatgcga
     4861 tatcgatcga atgaatcgta agagagaaca ttgatcccaa caagggaact ttgaaaacga
     4921 atcagccgat ggctgggcgc ctctcatgcc tgctgtgtag gtggcctgct gagcacccgc
     4981 cggagtgtat caatcaatac gtctttctca acaggcttgt ggagaaattc ttcagcgcct
     5041 agggatcgcg cctttgattc caatccatcg gtcgtctgcg cggtgatcac aatcacggga
     5101 accttcgcgg tgcgaaggtt ggctctgagc ctctcgagga ggagcaagcc tcctcctccc
     5161 ggcaagccaa tgtcgagcag gatgagattc ggctgttctc gcaccgctgt ggaggttgcc
     5221 tgcacgacat ccgtcgcgaa gaggcagacg atgccaaaat cctcgaggta ggttttgatg
     5281 agcagctgaa agtcggggct gtcgtcgata atcagtagtt tgagaggacg ctgaggtgtg
     5341 agcatatgtg aagtccatgt gagcactcgt aaacggtgac gctgtgcaca cagtacccac
     5401 gcccctcgct agaacacact aggaatgtcg ctaatttggc ggacagtgag cgacttctcg
     5461 atgccgaaaa cacgaatcag tcttctcact tccaagtcag cggaccgcga atgatcgaca
     5521 gacatcgcga tacaggtgca gaacgtgagt cccgcagcgc ccagacgtcg tcaagcctta
     5581 atggggggtt ttgcagttgg gatgactgca ggggcagatg gcggaaccgg tctgtccgct
     5641 ggcgacggat tcacattcag ggctgcaata gtcttcatct gtcacccgac atccacaccc
     5701 ataatgggcg caggtctgtc ctttttgttt gactgccgtg gtggtgctca tgagcttgcc
     5761 tcccggagga tgatgatgtc cccgctcctt gtaccgctat tcctccccga gaagacctag
     5821 caaatggcct agatcagggt cacatgcaca ggagctgatc agatttttgt agcagtgatg
     5881 gggctagtgg aggcgcaatt agaaaggggt caagacttgc tctcactccg atgatcggtc
     5941 agaacaaaag atcttcttca cctggcctgg gcggataggc tatggtgcag aatggctgtg
     6001 agaaatgtcg cgatcttcat cctctatacg tcctcgggac aactcctcct ccagcatcga
     6061 acccctgacg cctatcgatt acccaattac tgggccttct ttggaggtgg gatcgagcca
     6121 ggagaaagtc catccgacgc cctgaagcgc gaggtgatag aggaattgtc atatcacgta
     6181 caagacccgc actttctcat gacacaatta ttcagggaag gcgaagacga gaacacgaaa
     6241 tacgtgtttg ttgaacgata ccagggccaa ccgcttgaac taggagaagg gcaagccatg
     6301 ggctggtttc tccccgacga aacgcacgac ttgaaaatga tcgaccacga tcgtgccatt
     6361 gtggaacgag tgcggcagta tttgcgcgac cgctgagagt cgttacaaac caggttgctc
     6421 acgcctcatt ccgaggcagc cgatctccta cccgcagaac ccggttccgc ccgcctttca
     6481 gtcacgatac actccttagc agcgttccgc ctgttgccat tcgattgtgg gaccaaggac
     6541 caatcaaact cgcaacaaat tgcggtgact gccgtataat gcctgccctc aagaaatgat
     6601 cgccatgctt tcactgatgg gaataatcaa gcccttcgtt tgaccttcat ccaggagctg
     6661 accatgccgc gacgcacccg tgccgcttcc cctactcgac cagcccgagc caaagacgcc
     6721 gttctcgatt acccaggata cgccctcaaa tcaggtatcc tggcgccctc gacgattcgc
     6781 gccgtccaac agcgattgaa ccaggtcgga tgcgggccaa tctccgtgag cggcgtcttc
     6841 gatcgagcga cacaggaagc cgtgcggctc ttgcaagctc gctcggtcga tggacaaggg
     6901 caatcgctca agatcgatgg agtcgtcggg ccactcacct gggctgcact cttcggcgca
     6961 catcgggtgc cttcgatcgc caccacgccg atctccgcgt tgatgaaagc cactctcagc
     7021 attgccgcat cgcaggtcgg cgtgatggag gatccgtccg gttccaaccg cgggcccaaa
     7081 gtggatgagt atctaaaggc ggcgggaatc gatccttcca cggggagtta cgcctggtgt
     7141 gccgcattcg tctattggtc attcaagcag gcggcaactc ggctgggaac caccaatccc
     7201 gctattcgca cagccggcgt gctggaccat tggaagaaag ccggacaggc aggtgtcact
     7261 cgtttggtgg ccggtcaaat cctggacgat ctttccctgt tgaaaccagg actggtcttt
     7321 gtgatcaaca ccggcggtgg gcatggacat atgggtcttg tggaagactt tcgagatgat
     7381 cgcctggtca ccatcgaagg gaacacgaat cttcctggcg accgtgaagg tgtcggggtg
     7441 tttcgtcgaa gcgggcgtac catctccgat atcaatcagg gattcctgag ttacgattca
     7501 cagtcacatg cgagctcatg aagcggcctt gaacgaagac cggggctccg tcgtccaggt
     7561 cccatcggcc ctaccattct ccatacgaag tttgtactat gccaagcttt gatgggagga
     7621 gagtgcattg tcgaaccgac ccctccccgt cgagaaggac acataggtgc gggataaccc
     7681 ctcctcgcat accacaatcg actctttctt gactcatgca ggatatcgac tcaaaccctt
     7741 cctttacgct gactgacgtg cccggtggac tgccctggct tggacacctc cgctcattca
     7801 agcatcaacc gttgcgtaca atgtcgggat ggtggcgcca gcagggtgat gcggtgcggt
     7861 ttcgacttgg tcccaagacc attcatctct tcagccatcc cgcgttggcg gaagaggtgc
     7921 tgctctcaca agccgagcgg tttggaaaag tgtacgaaca gcaacgtccc accggactcg
     7981 cgctagtgct aggtaatgga ttggtgacga gttccgggga ggtgtggaag cggcaccgtc
     8041 gcatcattca accggtgttt catcgctctc gtatggccgc gatggcggat cgaatggtgc
     8101 aggtgggaga acaacggctc gccgggtggg cgactcatgg cgagcagccg gtagacatcg
     8161 ccgctgaaat gatgcagttg acgctcgaag tcatttccca aacgatgttt cataccagcg
     8221 tcgctcagca tatggatcag atcagtcatt ctcttcgcgt gagtttgaaa tatgcgttcg
     8281 actcatttca taacccgctg catctcccgg tctgggtccc aaccgctcgc aaccgtgagt
     8341 tcagtcgagc cctgaaattc atggatacgc tgatctacga attggtcgcg gcccggcgac
     8401 agagcggcgc gcactatgac gacctcctga acctgctgct ccaagctcgg gatgaggaaa
     8461 ccggcatggg actgaccgat caggaacttc gtgatgaagc cctgacgatc tttgcggcgg
     8521 gacatgaaac gaccgctaac gcactcgcgt ggacctggta tttgctcgcc acccatcccg
     8581 aggtgaggac acggtttcac acggaggtgg acctggttct ccgcgggcga accccgaccg
     8641 ccgacgatct gcaacatctg ccctacacac gtgcgatctt tgatgaatcg ctccgcctct
     8701 atcccccggt gccggccatc caacgcaaaa gcatgacgca gaccacgctc ggcggattta
     8761 ccctcccggc caatgccatc gtcctcgtcg gcttatataa cctgcaccgg catccggctt
     8821 tttgggcaga cccggaacag ttcctgcctg aacgttggct ggacggagaa cgtccgcctt
     8881 cccgctgcgc ctacctgccc ttcggcgtcg gaccgcgtgc ctgcgtgggc acgcacttcg
     8941 cgacagtaga aggccccctg cttttggcgc tgatcggtca acgctatgat cctcagctgg
     9001 cacaggacca cgtcgagccg gaagtcatgg tgacgttgcg gccgaaaaac ggcatccgta
     9061 tgacgcttca acagcggcat cagtctgtcg gctctgaata aagcgggtgg aagttcctcg
     9121 ggagctcact gagtggcagt ggagcctgac gcagattgga atcggaaaaa acggaaggaa
     9181 tccagaccga gggaaggaat ttggtcgggg cgagaggatt tgaacctccg acatcctgct
     9241 cccaaagcag gcgcgctacc gagctgcgct acgccccgac tgagtgctcc gccatcgctt
     9301 gttgcagcaa cgtcctcgct tgcgcaaagg cgcgggcccg atggctgatt cgattcttga
     9361 cctccggcgg caattgtccc aacgtctgat tatactccgg cagaataaag accggatcat
     9421 acccgaatcc gttggagccc acgaccgatt cggcaatgac cccttccaac aggcccttgg
     9481 ccgacatggt cttgccgcca ggaaacgcga tggcggccac ggtcacaaac cgagcccccc
     9541 gtcgcgcagg cggaatgcca ctgagctcct gcaataactt cctgcaattg tcttcgtagg
     9601 tcgcatgctc gccggcatat cgtgcagcga acgctcccgg ccgaccgccc agcgcatcga
     9661 cctccagccc cgtatcgtcc gcgaccgccg gcaatcccgt gaaacgcgag atctccacgg
     9721 ccttcttcat ggcattcgct tcgcaggtct caccgtcttc caccacttcc ggagcctcgg
     9781 gaaaatcatc gagggtccga atggtgatgt ccaagccgcc cagtagcgcg acaagttcct
     9841 gcttcttatg ctgatttctc gtggccagca cgatctgcat ggttagctca aggcgccgat
     9901 cagcgacttc tgcaaatcca ctaattcccg gatggcgccc caaccaagat ccagaaactc
     9961 atccatatct tttttgttga agggcgtgcg ttcggccgtt ccctgcactt caacatattg
    10021 tcccgccccg gtcatgacga gattcaaatc gacttcggca tgggaatctt cttcgtaggc
    10081 caaatccacc atcacctgac caccgacctt cccgatgctg atggccgcca aatagtcggt
    10141 caacggattg accttgagca gatccttctt tttcagcacg gtgatggcat ccgccaaggc
    10201 gatgaatgat ccggtgatcg aagcggtgcg agtgccgcca tcggcctgga tgacatcgca
    10261 atcgatccag atagttcgtt cacccaaccg tccggtatcg atgaccgcgc ggagagcccg
    10321 tccgaccagg cgttgaatct ccagcgtccg gccgccctgt ttccccttga ccgattcacg
    10381 cggcgaccga tcatgggtgg cccgtggcaa catggcatat tcggccgtca cccacccggt
    10441 gccctttcct ttgagaaagg gcggtacttt ctcctcgacc gacgcggtgc aaatcacctt
    10501 ggtgtcgccc atttcgatca ggacggagcc ttcagcatgc ttcgtaaaat tgcgcgtcac
    10561 tttgacgggg cggatttgat cccgtcgccg cccatcaatt cgccccaatc ctgccccgct
    10621 cgtcattcag tcgcgctcct tcctgcacgg caatccaaag gagcggatta tagtgaacgg
    10681 cccctcaaag agcaaatccc gacagccgaa cgagtccacc gatttctcaa actgcgtttg
    10741 aactggcatt cagcaagtgc ccctgtataa ttcaccacaa tggctgcata gattggctgt
    10801 ccgtaaacga cacattcccg acgtcagccg accgaccggc gtcagacttg acggccacgc
    10861 ccttaccgcc gactaggatc ctgtccggat ctggatcagt cctgctcatt gttgggccat
    10921 tcaatgtact tctgcagccc acggcaggga ggttgtattg gcacaaaagg tgcaaaattc
    10981 aggaaccagc taccatgact tcagaatgag ttggtaaaaa ccgatactga ttctatccct
    11041 gattcagcag cacttgacct taacccgcga gtgagccgta tgagcatccc gagcgtcagt
    11101 gaagtgacca cgcaacaatc cattctcgaa aaccgccata tcctgattgt ggacgacgaa
    11161 gagcccattc gccgcctgtt aggttatttg ctcgagccgc atggctacca ggtgaccctg
    11221 gccggtgagg ctcgcgaagc ccgtcaacgg atggaggacg ggtactacgc catggtgttg
    11281 tgcgatgtga acatgcctgg cgaatcgggc atggatctca ttcgccacat tttgacgcaa
    11341 tatcccaaca cggccgtcat catgatcacc gggctcgaca gcccggtcct ggccaatgcc
    11401 gccctggata tgggcgcctt cggatacatc attaagccct tcgaggcgaa cgaagtcctg
    11461 atcaacgtgg caaatgcgtt acgccgtcgc aagctggaaa tcgaaaatgc catgcatcgg
    11521 gaaaatcttg aagaggtcgt ccggaccagg acgattgcgc tgcaacaggc attggaatgg
    11581 ctggagcgga gcgaaaagga actgcggctc tcccgcgaag aaactattca acgactcgcc
    11641 atcgccgcgg aatatcgcga cagctcgacc gcacagcata tccaacggat gagccactac
    11701 tgcgagctgt tggcacgccg gtacggatta tcgcccgatc gatgcgatct catccgcacc
    11761 gccagcccca tgcatgatat cggcaagatc ggcaccccgg accatgtcct gctcaagccc
    11821 ggcaagttca cgccggagga attcaaagtc atcacgcaac atacagaaat cggctacagg
    11881 attctggccg ggtcggactc agacctcctg aaggtcgccg cgctcatcgc ctggacccat
    11941 cacgagcggt acgacggcac gggctatcct cgcgggatca agggggagga gattccactc
    12001 gaaggcagga tcgtcgcaat cgcggataac ttcgatgccc tcaccacgca gcgggtgtac
    12061 aagccggcgt ttgatttcga ccacgcaaag gaactgatgc tgaaggaacg gggcaaacat
    12121 tttgatcccg acctgctcga tatctttttc gaatccatgg ctgacgtcaa acggattttc
    12181 gatcaattcg ctgatccgag ctggctgtcc tcctcccggc atcgtgccat cactcatgac
    12241 cagggggaag ccgtatgaag cggacgtcac gaaactggat cgcctatctg tgtgagaatc
    12301 tggcctcctc cggcatgatg ccgacgtggg aagccgccac ctacctgacc gataatctgt
    12361 tcggggacaa gcccaacccg cgactcttgc gcacgacgtg cccgtacgag gatacgacgc
    12421 acttgctcca gcggttgtac cagccgctcg tcgccgcggc cacacgtgat atgccgctgc
    12481 cagcggccgc catgatccac gtgttcacgg atatcagcct cacgggaaaa tcggccgtat
    12541 tggtagtgta tttccccggc cccactcagc cgcatcagct cctgttgctg gatgccgcta
    12601 aagcctggga actggtgttt gagaattcag acgagttcaa tacctgggcg gaagcacgtt
    12661 accattcgat caaggcggcg cttgccaaga tcttaagcgg cgtagccccc tccccgactt
    12721 tgcccgcatc tctcgaagcc gtcaaaaact gaccatgacc ggcgagtgag ccggccctgt
    12781 cttgggccga ggcgtacgct gagagtaggt tcaacctgtg cgtgaggcaa gaacgaatct
    12841 gcaactcttc cattatcctg ctatgcgtcg tagttgagat tcggcgccaa ccaccgctcc
    12901 acctcgccca cagaaagttt cttccgcgcc gcatagtctt ccacctgatc gcggttgatt
    12961 ttccccacgg caaaatactt cgcctcgggg tgcgcaaagt agaggccgct gacagctgcc
    13021 gccggtaaca tcgcatagga ctctgtcaac gtcacacccg actgcgattc agcctgcagg
    13081 agatcgaaca ggattcgctt ttccgtgtgg tcgggacaag ccgggtaccc tggcgccgga
    13141 cgaatgccgc ggtaccgctc ccgaatcata tcctcgttgg tcagggtctc ggctttcccg
    13201 tatccccatt cacggcgaac ctgcttgtgc agccattcgg cgaacgcttc cgccaatcga
    13261 tcggccagcg ccttcaccat gatggagttg tagtcatcgt ggtccttctc atacttcgca
    13321 cagagcgctt ccacaccgat gcctgccgtc accacaaaag cccccacgta gtcgccacgg
    13381 ccagaatcct tgggggcaac atagtcggcc agggccaggt tgaactgatc ggccggtttc
    13441 tccgtttgtt gccggagtgt atgaaaggtg gtgaggacat cacggcgatc gccattccga
    13501 tacaattcga tatcatcgcc gacgctattg gccgggaaca atccatagac tccacgcgcc
    13561 gtgagtaatt cgccgcggat gatctcctcc agcaatgcct gggcatcggc cagcagttcc
    13621 ttggccttcg ggccgacagt ggcgtcttcc aatatctcgg gataccggcc gcgcaattcc
    13681 cacgtgtgaa agaacggagt ccagtcgatg aagggcacca gctcgcgcaa cggcatacga
    13741 tcgatctgcc gcagtccggt gaacgagggc gtgggaatgt cggctgaagc ccaatcggtc
    13801 ggcatccggt gggttcgcgc ggcctccacg gtgcggagtg ccttgctccc tcggtcctga
    13861 tgcgcctggc ggatgcgatc gtaatccgcg cgaatggaag cagaaaattc ctgttcctgc
    13921 tgcttgttga tcaggcttcc caccaccccg acggcgcgcg atgcgtccag cacatgcacc
    13981 gtcgtattgg cgtaggacgg cgcgattttc acagctgtgt gggccttact ggtcgtggca
    14041 ccgccaatga gcaatggcac cgtgaatccc tggcgcgtca tctccttggc cacatgcacc
    14101 atttcatcca atgatggcgt gatcaatccg ctcaggccca ccatgtccac ctgctgttca
    14161 cgagcggtct ccagaatttt ttcgcaggac accatgacgc caaggtcgat gacctcgtag
    14221 ttgttgcagc ccaataccac tcccacaatg ttcttgccga tatcgtgcac gtcgcccttg
    14281 acggtcgcga gtaacacctt gccgttggcg cgggaggtgc ccaatcgctg tttttctgcc
    14341 tccatgaaag gcatgaggta cgcgacagct ttcttcatga cccgggcgct tttcaccact
    14401 tgcggcaaaa acattttccc cgaaccgaaa agatcgccga cgatattcat gccggccatc
    14461 agcgcccctt cgatgacatc gagcggccga gcggatttct gacgcgcctc ctcaacatcc
    14521 tgatcgatgt agtccgtcag tcccttcacc agcgcatggg ccaaacgctc ctcaaccggc
    14581 cgctggcgcc actcatcgtc tttaaccgcc gctttgccct tttgcttgac cgtgtccgca
    14641 aacgtcacca accgttcagt cgcgtcggga cggcgattca acagcacgtc ctccaccagc
    14701 gcgagcaggt ccttcggaat ttcttcatac acggccagct gaccggcgtt gacgatgccc
    14761 atatccaggc cggcctgaat cgcatggtag agaaacgcgg catgcatcgc ctcgcgcacc
    14821 acgttgttgc cgcggaatga aaaggagata ttgctgacgc cgccgctgac cttggccagc
    14881 gggagatgcg ccttgatcca gcgcgtcgct tcgatgaaat cgacggcata attgttgtgt
    14941 tcttccaatc cggtcgcgac cgtgaggata ttcggatcga aaataatgtc ctgcggcgga
    15001 acaccgacct gttctgtcag cagtcgatag gagcgctcgc agatctcgat gcgccgcgcg
    15061 accgagtcgg cttggccacg ctcgtcgaac gccatgacga caatcgccgc gccatagcgc
    15121 cggatgaggc gcgcctgctc caaaaacttg gtctcgcctt ctttcaaact gatggaattg
    15181 acgatgcctt tcccttgaat gttgcggagc ccttcttcga tgacctccca cttggagctg
    15241 tcgaccatga tcggcacgcg cgcaatgtcc gactcggcgg ccaggagacg caggaacttc
    15301 tgcatcgcca ccttggagtc cagcaatccc tcatccatgt tgatatcgat aatctgcgcc
    15361 ccgccttcca cctgctggcg cgccacggtc agggccttgt cgtagtcgcc ggccagaatc
    15421 aatttcgaaa aggcgggtga tcccgttatg ttggtacgct cgccgacgtt cacaaagttc
    15481 gattccggtc gcacggtcaa cgcttcgagg ccgctgagac gtaaaaacgg ctcgaccttg
    15541 gccggccgac ggggcgacac cccgcgcacc gcttcc
//
LOCUS       DAOQEW010000083        15443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1062_length_15443_cov_9.333377, whole genome shotgun sequence.
ACCESSION   DAOQEW010000083 DAOQEW010000000
VERSION     DAOQEW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_15443_cov_9.333377"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLS35_10330"
     CDS             complement(<1..361)
                     /locus_tag="PLS35_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNI19105.1"
                     /translation="MYSFFKTDDSWAGLILRVVLGGVIFAHGAQKLLGWYGGFGFEGT
                     MGFFTQKMGFPWLVAFLVIIGESIGSLGLIAGLLTRFTAASFVVIMLGAIVTVHLPQG
                     FFMNWFGQQTGEGFEYHL"
     gene            complement(383..514)
                     /locus_tag="PLS35_10335"
     CDS             complement(383..514)
                     /locus_tag="PLS35_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19106.1"
                     /translation="MKDITLWVGWYGMLLTLPLFVAGLLLKDRTETAVLQWVRSHRR"
     gene            complement(714..2780)
                     /locus_tag="PLS35_10340"
     CDS             complement(714..2780)
                     /locus_tag="PLS35_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNI19107.1"
                     /translation="MSDRDSSGIERYRALLRVAEAIASHSDLSALVQDLARLLPLVVP
                     VNFVGLSLYDSQRGVMRLQVLRANVPAEIIGGHESDPQDTPAGLVWETQQPLLLNDLP
                     TERRWPKVIELMCEDGVRSCCLVPLTSPTQRLGSLEFSSLEQSAYSTSDVELMQQVGR
                     QVAVAVENVLNREAAAASQRDVERQRDRFSLLLRMTNSMASTLDLRQVFKAVSLCLRE
                     LVPQEYASLILCDGQTQRVRLHALDFPDNEGALTEGALSDVAGSLAGLAFERRRPAVA
                     NTLADLKAFSHAVPQLLVRKGFRSMCSLPLLSRDRVIGCLNLASHHEQAFGEQDLEFL
                     SQVAGQIALAVDNAVAYQEIHTLKDRLTEEKLYLEEEIRVEHGFDDIIGDSRPLKQIL
                     GQVEIVAPTDATVLILGETGTGKELIARAIHRLSGRHARTFVKLNCAAIPTGLLESEL
                     FGHERGAFTGAIAQKVGRFELAHGGTIFLDEVGEIPLELQSKLLRVLQEQEFERLGST
                     RTIRVDIRLVAATNRDLAKLVEEGRFRGDLYYRLNVFPLNLPALRDRREDIPMLVRYF
                     AQHYAARMKKTIESIPAPTLEALSRYHWPGNIRELENLVERAVILTPGTQLQVPLQEL
                     KIEARQAPAPGATLQDAERDQILRTLRDTRWVIGGPDGAAARLGLKRTTLTSKIKKLG
                     LSRPRE"
     gene            complement(2842..3570)
                     /locus_tag="PLS35_10345"
     CDS             complement(2842..3570)
                     /locus_tag="PLS35_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI19108.1"
                     /translation="MDDSGFPVKLIERRLIAEGTMAFHFAKPSQFVFTPGQFVDLTLL
                     QPSETDAAGNSRAFSIASAPHEDTIMVATRLRDTAFKRELQRMPIGSTVRMEGPFGKL
                     VLHSDQTRPAVLVAGGIGITPFRSMVVHAAQQQSPQRLVLFYSNRRPEDAAFLDELQS
                     LQDKNRHYSFVGTMTEPEKSLHAWRGETGYLNAGLLSKYLAEIDKPMYYVVGPPGMVT
                     ALRTILKDAHVEDTDIRIEKFAGY"
     gene            complement(3626..3862)
                     /locus_tag="PLS35_10350"
     CDS             complement(3626..3862)
                     /locus_tag="PLS35_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HNI19109.1"
                     /translation="MEEPVIAAKQPAVLELEAGTYYWCRCGRSKKQPFCDGSHKGTSF
                     TPMEFKTVEKKTLALCQCKHTKNPPFCDGSHKSL"
     gene            complement(4093..4539)
                     /locus_tag="PLS35_10355"
     CDS             complement(4093..4539)
                     /locus_tag="PLS35_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI19110.1"
                     /translation="MKAHYLGHVVFYVKDLQRALAFYRDLLGFHEVGRIFNGAAAALT
                     SGRTHHELLLIQVGDAPGPPPGRRRGLYHIGIKVGDSLDELRTAKRELEQAGIAIDGM
                     SDHTVSQSLYLRDPDGNEVELYVDADEAIWKTNPDAVVSPIKPLVL"
     gene            complement(4569..4961)
                     /locus_tag="PLS35_10360"
     CDS             complement(4569..4961)
                     /locus_tag="PLS35_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19111.1"
                     /translation="MSGQDHTTAHLDNVSRALLRLHKALLDAERVSYERVHGRIPTNG
                     AFFQLVLGDAWFAWLRPLSQLMARLDELSEEKEASDQGEITAVIGSVRTLLTPSEEGN
                     GFGRNYYDALQREPDVVLAHAAVRALLR"
     gene            complement(4991..6004)
                     /locus_tag="PLS35_10365"
     CDS             complement(4991..6004)
                     /locus_tag="PLS35_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNI19112.1"
                     /translation="MPDVRWTEIGLVEELKGKPLQEVVCGNTKLALSYRDGTFAAISG
                     ICNHIGGPLGEGRLEGDYVVCPWRLYVDLTSATKRRKKAKVPHPLARPIVRAEGPIRV
                     LGIATTAMTTDEPRYSASDALLEAALEHAREHLQLDTQLIRLRDLSFRPCEGFYSKAA
                     EACTWPCSITQMDAADQMDRVYEGIVHWADVILVSTPIRWGGASSLYYKMVERLNCIQ
                     NQETIANRHLLKDKVAAFIIMGGQDNVQGVAGQLMTFFAEVGCQFPQFPFIDHSRGWS
                     AEDMERNNREVQGSRELREGAEALVTRAADLARLMLEARLISAPLARGGRKAHQLDSE
                     PTG"
     gene            complement(6042..6500)
                     /locus_tag="PLS35_10370"
     CDS             complement(6042..6500)
                     /locus_tag="PLS35_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNI19113.1"
                     /translation="MKNLEQVRTLAKDLTKTYPRSPREMLGGYVIGARCADKCRAFLL
                     GINGEYNYWPCSLAALLFTFTGINPDQFKEVVATGATDEELGDWLKEHSKVQDRQAII
                     EWNNTMRDLRLSEMAPAVQAYMEDYIPKYVPSHRPVYTYFDVYDLEEQRL"
     gene            complement(6497..6943)
                     /locus_tag="PLS35_10375"
     CDS             complement(6497..6943)
                     /locus_tag="PLS35_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI19114.1"
                     /translation="MSVQVYGCNHIVIEVTDAKKAVKFYSDVFGLEMLRGGEGAAWCK
                     LGEHQFLAIFEVKTLQPDRMKHFGLMVRDADQIKEVRRKLTKKYKLKMEPHFRCDFRD
                     PWGNRIQVGDLSDESLVWLLPYQEVQKAGITFAKPSRAGSGKGATT"
     gene            complement(6956..7597)
                     /locus_tag="PLS35_10380"
     CDS             complement(6956..7597)
                     /locus_tag="PLS35_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNI19115.1"
                     /translation="MTAPVLTIEVYSDVVCPWCYVGKRRLEQALESTGRQDSALVFWR
                     PFQLNPTMPKAGMDRRVYLEAKFGGPEEMQAIQNRIAGAGVSAGIEFAFDRIARTPNT
                     FEAHRLIWFAQQQGRQDAAMEELFHGYFTDGLNIGADEVLVSLAARAGLDGAAVGRFL
                     QSQDGVEAVRQEEARGRQLGIRGVPYFLLNGKTVVSGAQPAETFLQAIEHTTR"
     gene            complement(7594..8478)
                     /locus_tag="PLS35_10385"
     CDS             complement(7594..8478)
                     /locus_tag="PLS35_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNI19116.1"
                     /translation="MRTETMNAKELVGVYQAGSHHMVGDGFPVRNMIPGAGVEEQLSP
                     FLMLDYMGPEEFPPTTRRLGVGEHPHRGFETVTIMYHGKVAHRDSTGSGGVIGPGDVQ
                     WMTAASGVVHEELHEKEFAEKGGLLEGVQLWVNLPKAFKMTAPRYQTLLSSDIPMVEL
                     GGGAGRLRVIAGEFRGVAGPAKTFSPVHLYDLRLAAGHRLELDLPEGFNSSLFVLHGQ
                     VVVNGSQSVDDVHIALFGQRGERVVLEAKQEATILVLSGKPIAEPIARYGPFVMNTRD
                     EIIQAVQDYQAGKMGHLS"
     gene            complement(8493..9350)
                     /locus_tag="PLS35_10390"
     CDS             complement(8493..9350)
                     /locus_tag="PLS35_10390"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNI19117.1"
                     /translation="MLVVLGATGHTGSVVAQTLLTHKQPVRVVVRSADHGATWAAKGA
                     EVAVASLDDQAALTKALQGASGVYLLVPPNYGATSWLAEQRRRVDQTAEAVKATRIAH
                     VVLLSSIGAHLPEGTGPIRAARYGEHRLTAVAPNVTILRPGYFMENWANGIGMAKAEG
                     LLPSFIPPKMSMPMISTRDIGRVAAERLIAGGEGHAIVELAGPEEYSPEEVASELGRI
                     FGRGVSVQAAPLSAVVPTFTSFGFSTEAAGLFQEMYTAFSKDVVRYERPQSLVRGTKA
                     LAEALREMA"
     gene            complement(9709..10188)
                     /gene="smpB"
                     /locus_tag="PLS35_10395"
     CDS             complement(9709..10188)
                     /gene="smpB"
                     /locus_tag="PLS35_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNI19118.1"
                     /translation="MTKTHDTDDQYKVVATNRKAYHDYFVEEKFEAGVILRGTEVKSL
                     REGRVNLQDSYASVDDGEVYLNHCHISPYSHGNLMNHDPLRKRKLLLHRKEINKLIGK
                     TQLKGLTLVPLRIYFSKRGHAKVELALAKGKKQYDRRESIKAREAGREVERAIKSRK"
     gene            10241..10738
                     /locus_tag="PLS35_10400"
     CDS             10241..10738
                     /locus_tag="PLS35_10400"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNI19119.1"
                     /translation="MRGQSRLDSFGTILAGVAHRLGLESKLFEARLRRHWPEIVGEPI
                     ATHTRPDQIRFKKLHVYVHNSVWLQQLTFLKPVLLEKVNGMAGETLVTEIVLRIGEFS
                     AERKVSPPAATVEEPVPQPSAELLQETALHTQNIQDPALREQLAAVMAQALSGIPPDS
                     SRRAP"
     gene            complement(10683..11297)
                     /locus_tag="PLS35_10405"
     CDS             complement(10683..11297)
                     /locus_tag="PLS35_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19120.1"
                     /translation="MVMNQQQPPSAGTSAGRPPKRPLDTVVKLALGVLFSSFALIWGG
                     MFLSRPDRSIPPYSIGSQEGSIVAVHVPSWTSDTEIQTLIERFRKVGTETRNFGPMKI
                     RPTTADDPKGRYQRIAIYIFTDEGWTEPEMLHKYVTGEDPAVRDGFEGSARGYYKLDE
                     QEEEGRIGPVPKGKDSAATAAYSRQLFKGPVATSPAVSPTTPAP"
     gene            complement(11337..13796)
                     /gene="gyrA"
                     /locus_tag="PLS35_10410"
     CDS             complement(11337..13796)
                     /gene="gyrA"
                     /locus_tag="PLS35_10410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNI19121.1"
                     /translation="MPPDERLGQIAIEDEMRSSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILHGMNEMGLAANRPYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQNFNMRYMLV
                     DGQGNYGSMDGDAAAAMRYTEARLTKLAEELLADIEKETVDFGPNYDESRVEPLVLPS
                     RVPNLLVNGAGGIAVGYATNIPTHNLGEVIEGLLLLLENPEVTIAQLMKKIPGPDFPT
                     AGFIYGTSGIKDAYETGRGLLTVRAKVAIETDERTDRERLIITEIPYQVNKSKLIEKI
                     ADLAQEDRVTGISDIRDESDREGVRVVIELKRNEIPLVVLNNLYKHSQLQTTFGVNML
                     ALVNNRPEVLNLKRILEAFVEHRREVVVRRTAYDLRKAEERAHILEGLKIALDNLDAV
                     IALIRRSQSPDVARAGLMQQFRLSEIQANAILEMRLQRLTQLERDKLVEEYREVLKTI
                     EYLRSVLGSEALVRKIIRDELTEIKEKYQDERRTKIVKEEAELTLEDLIAEEEVVVTI
                     SHAGYIKRNAVTLYRAQRRGGKGKIAMGIKEEDFVETLFTASTHDSLLFFTDAGKVYW
                     LKVHEIPEASRAAKGKALVNLLALSKDEKVTATLPVKEYRADRFVIMGTKQGVIKKTE
                     LSAYSNPRQGGIIALSLDAGDKLIGVDLTDGQREILLGTKQGITIRFKEEDVRAMGRT
                     AHGVRGITLEAGDEVIGMETITPDSTTAILTVTEGGYGKRTPVNEYRVQGRGGKGIIS
                     VKTTERNGAAVGFLQVRDEDEIMLMAAQGKVLRCKVDDIREIGRNTQGVRLLDMDGDE
                     DRVVAVVRLVEREEGVPEEGE"
     gene            complement(13975..>15443)
                     /locus_tag="PLS35_10415"
     CDS             complement(13975..>15443)
                     /locus_tag="PLS35_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNI19122.1"
                     /translation="VRNPQFEGQTKAKLGNSEVKGIVEAAVNDALGTYFEENPPVARK
                     IIGKAIDAARAREAARKAKDLIRRKSALDGGSLPGKLADCSEKDPALSELFIVEGDSA
                     GGSAKQGRDRKFQAILPLKGKILNVEKARFDKMLSSDEIRTLILALGTGIGRKKEDSD
                     KADKDAFDIARTRYHKIVLMTDADVDGSHIRTLLLTFFFRQMPELLERGYIYIAQPPL
                     FKVKKGKTERYLKDEPAMNEYLADLAVEGVEVALENGREFVTGRRLLPILKKLIAFES
                     LLHKVNKKHHEANMLRAFVDEPALTREVLKDPASLRTVVDHVKATLALVYPKAEPTIE
                     ILEDEEHQSSKLVCKVATGGIAYELQVTHELVGSADFRELQKQAPSSIGLGKPPYKIK
                     IKEGEVLKNGSAELVQAILEEGKQGLNIQRYKGLGEMNPGQLWETTMDPEKRTLLRVN
                     LEDMTGVDEIFTILMGDEVEPRRNFIQQHALEVRNLDV"
BASE COUNT         3052 a         4904 c         4161 g         3326 t
ORIGIN      
        1 gcaggtgata ttcgaaccct tcaccggtct gctgtccgaa ccagttcatg aagaagccct
       61 ggggcaagtg caccgtcaca atcgccccca acatgatcac cacaaagctc gccgccgtga
      121 accgcgtgag caggccagca atcagcccga gactaccgat ggactctccg atgatgacga
      181 ggaatgcgac caaccaaggg aagcccattt tttgagtaaa aaatcccatg gtgccctcaa
      241 acccgaaccc tccgtaccat cccaatagtt tctgggcgcc atgggcgaag atcacacctc
      301 ccagcaccac ccgcaaaatc aaaccggccc aggaatcatc tgttttgaaa aagctgtaca
      361 tgtcacacct ccttgatatg aattaccgcc ggtgactccg cacccattgc agaaccgccg
      421 tctccgtcct gtctttcagc aacagccccg ccacgaagag gggcagggtc aggagcattc
      481 cgtaccagcc gacccagagc gtgatgtctt tcatgaattc cccccgttcc cttgtgcgtt
      541 gctatgcagg ggtaatggca aggcctgttc caggcgctgg acgatcacgc cacatgggct
      601 aactgcttga aatcctgatc tgattgtccg tgggtcgaag aaaacgatgg gctgacaaaa
      661 gatgcgtgac gaagggcgtg cgacgagacg tcgtcaggtg acgaaacggc tcgtcactct
      721 cgtgggcgag agagtccgag ctttttgatt ttcgatgtga gggtagtgcg tttgagacct
      781 aatcgagccg ccgcaccatc cgggccgccg atcacccatc tggtgtcgcg cagggttcgg
      841 agaatttggt cgcgttcggc atcctgcagg gtggctcctg gtgccggggc ctgccgcgcc
      901 tcgatcttca gttcctgaag cggcacctga agctgcgtcc ctggagtcag gatcacagct
      961 cgctcgacca gattctccaa ttcgcgaatg ttgccgggcc agtgatatcg ggacagggcc
     1021 tccagcgtcg gcgcgggaat ggattcaatg gtcttcttca tgcgcgcagc atagtgctgc
     1081 gcgaaatacc gcacgagcat gggaatgtcc tcccgtcgat cccgtagcgc cggaaggttg
     1141 agcggaaaca cgttcagccg gtaatacaaa tcgccgcgaa accggccttc ctcgaccaat
     1201 ttggccaggt ctcgattcgt ggcagctacc agacggatat ccacgcgaat cgtgcgcgta
     1261 ctcccgagac gctcgaattc ctgctcctgc agcacccgga gcaatttgga ttgcagctcc
     1321 agaggaattt ctcccacttc gtccagaaag atcgtgccgc catgggccaa ctcgaaccgg
     1381 ccgacttttt gggcgatggc gcccgtgaat gcgccccgtt cgtggccgaa caattcactc
     1441 tccaacaatc cggtcggaat ggcggcgcag ttgagtttaa cgaacgtgcg cgcatgccgg
     1501 ccactcagac gatggatcgc cctggcaatg agttctttcc cggtaccggt ttcgcccaga
     1561 atcagcaccg tcgcgtcggt gggcgccacg atctcgactt gccccaaaat ctgcttcaag
     1621 ggccggctgt cgccgatgat gtcgtcgaag ccgtgctcga cacggatttc ctcttccaga
     1681 tacaactttt cctcggtcaa tcgatcttta agcgtgtgaa tctcctgata cgccacagca
     1741 ttgtcgacgg ccaaggcgat ctgccccgcc acctggctca agaattcgag atcctgttcg
     1801 ccaaaggctt gttcgtgatg actcgccaga ttcaggcagc cgatcacgcg atcgcgcgac
     1861 agcagcggca gtgagcacat ggagcggaac cctttcctca ccaggagttg aggcacggca
     1921 tgcgaaaacg ccttcaggtc agccagggtg ttggcgaccg cggggcgcct ccgctcaaac
     1981 gccagaccgg ccagagaccc ggcgacatcc gatagtgccc cttcggtcaa cgcgccttcg
     2041 ttgtcgggaa aatcgagcgc atgcagacgg acgcgctgcg tctggccgtc gcagaggatc
     2101 aaactggcgt attcctgagg gaccagctcg cgcagacaga ggctcaccgc cttgaacacc
     2161 tggcgcagat ccaaggtcga cgccatcgaa ttcgtcatgc gcagcaacag actgaagcga
     2221 tcccgttgcc gttccacgtc ccgttgtgat gccgctgccg cctcgcggtt cagcacgttt
     2281 tctactgcca ccgccacttg gcgtccgact tgctgcatca attccacgtc cgacgtgctg
     2341 taggcgctct gctcaagact ggaaaactcc aaagatccca gtcgctgcgt cggggacgtc
     2401 agcggcacca gacaacatga acgcactccg tcctcgcaca tcaattcgat gaccttcggc
     2461 cagcgtcgtt ctgtgggcag atcgttcagc aagagtggct gctgggtttc ccacaccaga
     2521 cccgccggcg tgtcttgggg atcgctttcg tgccctccga tgatctccgc aggcacattc
     2581 gcccgcagca cctgcagacg catcacccca cgctgagaat catagagaga cagtccgacg
     2641 aaattgacgg gtacgaccaa gggcagcagc cgcgcaagat cctgcactag cgcagacagg
     2701 tcggagtgac tggcaatggc ttccgcgacg cgcaatagcg cccgataccg ctcgatgccg
     2761 gaggaatctc tgtcgctcac cgaggtcctg tcatgtgtca tcacggaatg agcaacgtcg
     2821 ttcgtgacgc tgcagcctgc gttagtaccc cgcgaacttt tcgatgcgaa tatcggtgtc
     2881 ctcgacgtga gcgtctttca gaatggtccg caatgcggtc accatgccgg gcggaccgac
     2941 cacatagtac atcggcttgt cgatctccgc gagatacttg gagagcaagc ccgcattcag
     3001 gtaccccgtt tcgcctcgcc aggcatggag ggatttctcg ggttcggtca tggtacccac
     3061 aaaactatag tgacgattct tgtcctgtaa ggactgcagc tcatccaaaa acgccgcatc
     3121 ctccggtcgc cggttggagt agaacagcac cagacgctgc ggtgattgct gctgggcggc
     3181 atgcaccacc atgctgcgga aaggggtaat cccgatgccg ccagctacca agacagcggg
     3241 gcgagtctgg tcactgtgca acacgagttt gccgaacggc ccttccatcc ggacggttga
     3301 gccgatcggc atacgttgca actcgcgctt gaaggcggtg tcgcggagac gcgtcgcgac
     3361 catgatggtg tcctcatgcg gggcgctggc gatggaaaag gctctgctgt tgccggccgc
     3421 atcggtttcc gatggctgga gtaacgtcag gtccacgaac tgtcctggag tgaacacaaa
     3481 ctgagacggc ttggcgaaat ggaacgccat cgttccctcc gcgatcaacc gtcgttcgat
     3541 cagtttcacc ggaaagccgc tgtcatccat tgcgtcactc atccctgacc tcgcatgaca
     3601 ttacaggcga tacgtcccca ccctgttaca gcgacttgtg cgagccatca caaaacggag
     3661 gattcttcgt atgcttgcac tgacacaagg ccagggtttt cttttcgacc gtcttaaact
     3721 ccatgggcgt gaagctggtt cccttgtgag atccatcgca aaatggttgc ttcttcgagc
     3781 ggccgcagcg gcaccaataa tatgtccctg cttccaattc caatacggcc ggctgcttcg
     3841 ctgcaatgac tggttcttcc atgtgcatct cctcctggtt gactgactgt tttcatgtcc
     3901 acatacgccc ttgccctgcc tagtcccgct cgatgcatgt catgaatcgc acatcgcttg
     3961 cccctggaac aggtgatgag ctcgtcccgc cacgcggctg catcacagaa aagcacattg
     4021 ccgcggaaga atgtgcccat ctccagccgg ctgttgcgac agaaggggac agcgtgaatc
     4081 tggccttcag cgctaaagca ccagcggttt gatcggcgag acgaccgcgt caggattagt
     4141 tttccagatc gcctcgtccg catcgacata cagctccacc tcgttgccat ccggatcccg
     4201 aaggtacaga ctctgactga cggtgtggtc actcatgccg tcgatcgcga tgccggcctg
     4261 ctccagctcg cgtttagccg tcctcagttc gtccaggctg tccccgactt tgatgccgat
     4321 atggtagagc cctcgtctcc gtcccggcgg cggtcccggt gcatcgccga cctggatcaa
     4381 cagcaattca tggtgcgtgc ggccggatgt cagcgcggct gcagcgccat tgaagatccg
     4441 cccgacttcg tgaaacccta acaaatcgcg gtagaacgcc agcgcacgct gcagatcttt
     4501 cacgtaaaac acgacatgcc ctaaatagtg cgctttcatc gtcgtgactc cttcctcggc
     4561 ctgtcccgtt atcgaagcag cgcccgaacc gccgcgtgag ccagcaccac atcaggctcc
     4621 cgttgcaagg catcataata gttccggccg aaaccattgc cctcttcgga aggggtcaac
     4681 agggtccgca ccgacccgat caccgcggtg atctcccctt gatccgacgc ctctttttct
     4741 tcggacaact catccaatcg cgccattagt tgggagagcg gacgcaacca ggcaaaccag
     4801 gcatccccga ggactaactg aaaaaacgcg ccattggtag gaatgcgccc atgaacccgc
     4861 tcgtaggaga ctcgttcggc atccaacaac gccttatgaa ggcgcaacag ggcgcgagac
     4921 acgttatcca gatgcgccgt ggtatgatcc tgacccgaca tacgtccgcc tccttacgat
     4981 tggctctcca ttatcccgtc ggttcactgt cgagttgatg cgccttgcgg ccgcctcggg
     5041 cgagcggagc ggagatcagg cgcgcctcaa gcatgagccg agccaggtcc gctgcgcggg
     5101 tgacgagcgc ttccgcccct tcgcgcaatt cgcgactgcc ttgcacctcc cgattgttcc
     5161 gttccatgtc ttccgcactc cacccacgcg agtggtcgat aaagggaaat tgcggaaact
     5221 gacaaccgac ttcggcgaaa aaggtcatca attgtccggc cacgccctgc acattatcct
     5281 gtccgcccat gatgatgaag gcggccactt tgtccttgag cagatgccgg ttcgcgatcg
     5341 tctcttgatt ctgaatacag ttcagccgtt cgaccatttt gtaatacagg ctgctggccc
     5401 cgccccatct gatcggggtc gacacgagaa tcacgtctgc ccaatggaca ataccttcat
     5461 agactcgatc catttgatct gccgcatcca tttgggtgat cgaacagggc caggtacagg
     5521 cttcggccgc tttcgaataa aacccttcac aaggccggaa cgacaggtcc cgcagccgga
     5581 tgagctgcgt gtcgagctgc aagtgctcgc gcgcatgctc cagggccgct tccagcagcg
     5641 catcggacgc gctgtatcgc ggctcatccg tcgtcatggc cgtcgtggct atcccaagca
     5701 ctctaatggg accctcggca cgcacgatcg gacgggcgag gggatgcggc accttcgcct
     5761 tcttccggcg tttcgtggcc gaggtcagat ccacatagag acgccagggg cagacgacat
     5821 agtcaccttc gagccgcccc tcgcccaacg ggccgccgat gtgattgcag attccagaga
     5881 tggctgcgaa cgtgccgtcc ctatagctga gggcgagctt cgtattgccg cagacaactt
     5941 cctgcagtgg cttccccttc agttcctcaa ccaacccgat ctctgtccac ctcacatccg
     6001 gcatgtcaca ttcctatcgg cgtccgcggc agggctgccc gtcacaagcg ctgctcttca
     6061 agatcataca catcgaaata ggtgtaaacg gggcggtggc tcgggacata cttcggaata
     6121 tagtcttcca tataggcctg taccgcggga gccatctcgg aaagacgcag atcccgcatc
     6181 gtattattcc attcaatgat cgcctgccga tcctgcacct tgctgtgctc cttgagccag
     6241 tcgccgagtt cctcgtcggt cgctcccgtt gcgacaacct ccttgaactg gtccggattg
     6301 atccccgtaa aggtgaacaa caacgcggcg agcgaacaag gccaatagtt atattccccg
     6361 ttgatgccca ggaggaacgc ccggcactta tcggcacaac gagcgccgat aacataccct
     6421 cccagcattt ccctcgggct gcgaggatag gtcttggtga gatctttcgc aagcgtgcga
     6481 acctgctcca gattcttcat gtggtcgctc cttttcctga tccggcacgc gagggcttgg
     6541 cgaacgtgat gccggccttt tggacttcct gatagggcag cagccagacc agcgattcgt
     6601 ccgacaagtc tcccacctgg atccggtttc cccaggggtc gcgaaaatcg caacggaaat
     6661 ggggctccat cttcagctta tatttcttgg tgagcttgcg ccggacctct ttaatctgat
     6721 cggcatcgcg caccatcagt ccgaaatgct tcatgcgatc cggctgcaac gtcttcactt
     6781 cgaagatggc cagaaattga tgctcgccca gtttgcacca ggcagcaccc tctcctccgc
     6841 gtaacatctc caagccgaac acatccgaat aaaacttcac ggcctttttc gcgtcggtga
     6901 cttcgatgac gatatggttg catccgtaga cctgaaccga catgtccatt gctccttacc
     6961 gggttgtgtg ctcgatggcc tggaggaagg tctcggccgg ctgcgcaccg gacaccacgg
     7021 tttttccatt caagaggaag tacgggaccc ctctgattcc caactggcga ccgcgtgcct
     7081 cttcctgtcg caccgcctcg accccgtcct ggctctgcag gaaccgaccg accgctgccc
     7141 cgtccaggcc ggcccgggca gcgagagaca ccagcacctc gtccgctccg atattcagcc
     7201 cgtcggtgaa gtatccatga aagagttctt ccatcgcggc gtcctgacga ccctgttgct
     7261 gagcaaacca gatcagccga tgggcctcga aggtattcgg cgtgcgagca atccggtcga
     7321 aggcgaactc gatcccggca cttacgcctg cgcccgcaat tcgattctga atcgcctgca
     7381 tttcctccgg accgccgaac tttgcttcaa ggtatacacg acgatccatg ccggccttcg
     7441 gcatggtcgg attcagttgg aacgggcgcc aaaacaccag agcagagtcc tgtctgcccg
     7501 tcgattccag cgcctgctcc aaccgtcgct tcccgacata acaccaggga cagaccacgt
     7561 cggaatagac ctcgatggtc agtaccggcg ccgtcatgac aaatgtccca tctttcccgc
     7621 ttggtagtcc tgcacggcct ggatgatctc atcgcgtgtg ttcatcacaa acggaccgta
     7681 gcgggcgatc ggctccgcaa taggcttgcc gctcagcacg aggatggtcg cctcctgttt
     7741 cgcctccaga accacacgct cacctcgttg tccgaacagc gcgatgtgca cgtcgtccac
     7801 tgactgagac ccgttcacca ccacctgccc atggagcacg aacagggacg aattgaaccc
     7861 ctcgggcaag tcgagttcca gccggtgccc tgccgccaaa cggaggtcat acaggtgcac
     7921 cggactaaac gtcttcgccg gtcctgccac accgcgaaat tctccggcaa tgacgcggag
     7981 ccggccggcg cctccgccaa gttccaccat cggaatgtcg ctgctcaaca acgtctgata
     8041 ccgcggcgcc gtcattttga acgccttggg cagattcacc cacagttgaa ccccttccaa
     8101 gagaccgccc ttttctgcaa attctttctc gtggagctcc tcgtgcacga cgcctgacgc
     8161 ggccgtcatc cactgcacat cgccgggccc gataacaccg ccgctccccg tcgagtcacg
     8221 atgcgccact ttcccgtgat acatgatcgt cacggtctcg aagcctcgat gcggatgttc
     8281 ccccacacca agccggcgcg tggtcggcgg aaattcttcg gggcccatat agtcgagcat
     8341 caaaaaggga gacagttgct cctcgacccc tgcgcccgga atcatattgc gcacgggaaa
     8401 gccgtctcca accatatgat gggagcctgc ctggtagact ccgacgagtt cttttgcgtt
     8461 catggtttct gttcgcatgt cggcctcctt tcttacgcca tctcccgcaa tgcttctgcg
     8521 agagccttcg ttccccgcac gagtgactga gggcgctcat acctgaccac gtccttggaa
     8581 aacgcggtat acatctcttg aaacaatccg gccgcttcgg tggaaaaccc gaatgaggtg
     8641 aatgtcggca ccactgcgct caacggcgcc gcctgtacgg agacgcctcg tccgaaaatc
     8701 cggccaagct cagaggccac ctcttcagga ctgtactcct ctggacccgc tagttccaca
     8761 atcgcatggc cctcgcctcc ggcaatcaat cgctccgctg ccacacgacc gatgtcccgg
     8821 gtagatatca tcggcatcga catcttcggc ggtataaacg acggcaacag accctccgcc
     8881 ttggccattc cgataccatt ggcccaattc tccataaagt aacctggccg caagatcgtg
     8941 acgttgggcg ccacggccgt gagccgatgt tctccataac gagcggcgcg aatcggcccc
     9001 gtgccttccg gaaggtgggc gccaatagaa gacagcagga ccacatgcgc aatcctggtc
     9061 gccttcacgg cctctgcggt ttggtctacc cgccgccgtt gctcggccag ccatgacgtt
     9121 gcaccatagt tcggcggcac caggagatag acgcccgatg cgccttgcaa tgccttcgtc
     9181 agagccgcct ggtcatccaa cgaggccacg gcgacttccg ctcccttcgc agcccaagta
     9241 gcaccgtgat cggccgaccg taccactact cgcaccggct gcttgtgcgt cagcagggtc
     9301 tgcgcgacga ccgatcccgt atgtcctgtc gcacccagta caaccaacat cgttgtcgtt
     9361 cccttctttt cctgaattgc cccatgacac gaccgctgaa ccgtcggcat cggccggctc
     9421 ccatcgccgg caggagaaga cggccacccc cacttcgctg caccccacta taatagttct
     9481 agttagaaca aagtcaagtg caaatatgcc ggggctcctt cactttctga gttgatgcat
     9541 ggacaggctg taagacatga ccacgataga agacccaccg acgatacgct cgagccgtta
     9601 cacgaggtgg gggcagtcag cagcagcggg aggacgagat tacgacgacg atcagcggag
     9661 cagggaagca ggcgagggtc tacagatcaa tgacgaaggt gagagaggct atttccgact
     9721 cttgatcgcc cgttcgactt cgcggccagc ctcacgagcc ttgatggact cacggcggtc
     9781 gtactgcttt ttgcctttgg ccagggccag ctccactttc gcatgaccgc gtttcgaaaa
     9841 gtagatgcgg agagggacga gagtgagtcc cttgagctgg gttttcccga tcagtttatt
     9901 gatctccttg cgatgcagca ggagcttccg tttgcggaga ggatcatgat tcatcaggtt
     9961 gccatgcgag taggggctga tgtggcaatg gttgaggtac acctcgccat catcgaccga
    10021 cgcataactg tcctggaggt tcacgcggcc ctcgcgcagc gatttcacct cggtgccgcg
    10081 caggatcacg ccggcctcaa acttctcttc gacaaaataa tcgtggtagg ccttcctatt
    10141 tgtggccacc accttatact gatcgtctgt atcgtgcgtt ttggtcatgt tgctgaggcg
    10201 accgctgttt cactcgcgcg cattttgata tgatgccgcc atgcgcggac agagcagact
    10261 cgattccttc ggcactattc tagcaggtgt cgcccaccgg ttgggcctcg agagcaaact
    10321 cttcgaggcg cgtctacgac gccattggcc agagattgtg ggcgaaccga tcgcgaccca
    10381 cacgcgcccc gatcaaatcc gattcaagaa acttcacgtc tacgtccata actctgtctg
    10441 gctgcaacaa ctcacattcc tcaagccggt acttctcgaa aaagtgaatg ggatggccgg
    10501 ggagacgctc gtgacggaaa tcgtcctgcg catcggggag ttttccgccg agcgcaaagt
    10561 gagcccgccg gcagcgacgg ttgaagagcc tgtccctcag ccgagcgccg aattgctcca
    10621 ggaaacggcc ctgcatacgc agaacattca ggatcctgct cttagggagc aacttgcggc
    10681 cgtcatggcg caggcgttgt cggggatacc gccggactcg tcgcgacggg ccccttgaac
    10741 aactgcctcg aataggccgc cgttgccgca ctgtctttgc ccttgggcac cggcccgata
    10801 cggccttctt cttcctgttc gtccaacttg tagtatcccc gtgccgaacc ctcgaatccg
    10861 tcccgcaccg ccggatcttc gcccgtcaca tacttatgca gcatttcagg ctccgtccat
    10921 ccctcgtcag tgaagatgta aatcgcgatg cgttggtagc gccctttcgg atcatctgcg
    10981 gtggtcggac ggatcttcat ggggccgaaa tttcttgtct ccgtccccac cttgcggaat
    11041 cgttcgatta aggtttggat ctcggtgtcg ctcgtccagg acgggacgtg caccgcgacg
    11101 atgctacctt cctgggagcc gatgctgtag ggaggaatgg aacgatccgg ccggctcaga
    11161 aacataccgc cccagatcag agcaaaggag ctgaacagaa cgcccagcgc cagtttcacg
    11221 accgtatcca ggggacgttt cggaggacgg cctgcggaag tcccggcgga cggcggttgc
    11281 tgctggttca taaccatggt gaaggacgcc tgaatccgcc ggcgttcacg gagtgactac
    11341 tcgccctctt cgggcacccc ttcttcacgt tcgacgagcc gcaccacggc gacgacacgg
    11401 tcctcatcac catccatatc aagcagacgc acgccctggg tgttgcgtcc gatttcacga
    11461 atgtcgtcga ccttgcagcg cagcactttc ccctgcgcag ccatcagcat gatttcgtcc
    11521 tcatcccgga cctggaggaa tccaacggcc gcgccgttcc gttccgtagt cttgacgctg
    11581 atgatgccct tgccgccacg tccctgcacg cgatactcgt tcaccggcgt gcgcttcccg
    11641 taaccgcctt cggtcaccgt gagaatcgcc gtcgtggaat cgggcgtgat ggtctccatg
    11701 ccgatcacct catcacccgc ctccagagta atgcctcgca caccgtgggc cgtacgtccc
    11761 atggcccgca catcttcttc cttgaaacgg atggtgatgc cctgcttcgt gcctaacaga
    11821 atttctcgct gaccatcggt aagatcgaca ccgatcaatt tatcacccgc atcaagcgag
    11881 agggcgatga tgccgccctg gcgaggattg ctgtaggccg acagctccgt cttcttgatg
    11941 acgccctgct tggtccccat gatgacaaac cggtcggcgc ggtattcttt caccggcagc
    12001 gtggctgtga ctttctcgtc cttggagagt gccagcagat tgaccaacgc tttgcccttg
    12061 gccgcccggc tcgcctcggg gatttcgtgc accttaagcc agtagacctt gcccgcatcg
    12121 gtaaagaaca gcagcgagtc atgcgtggag gccgtgaaga gagtttcaac gaaatcctct
    12181 tccttgatgc ccatggcaat cttccctttg ccgccccgcc gttgcgcgcg atagagcgtg
    12241 accgcgttcc gcttgatgta cccggcatgc gaaatcgtca ccacgacttc ttcttcggcg
    12301 atcaagtctt cgagcgtcag ttccgcttct tccttgacga tcttcgtgcg gcgttcgtcc
    12361 tgatacttct ctttgatctc cgtcaattca tcccggatga tcttgcgcac caacgcttca
    12421 ctgccgagga ctgaccgcag gtattcgatg gtcttcaaca cttcgcgata ctcttccacc
    12481 aacttgtcgc gctcgagttg cgtgagccgc tggagacgca tttcgagaat cgcgttggct
    12541 tggatttcag acaaccggaa ctgctgcatc aaacccgcgc gcgccacatc gggcgactgc
    12601 gagcgccgga tcagggcaat cacggcatcc aggttgtcca gggcgatctt gagcccttcg
    12661 aggatatggg cacgttcttc cgccttgcgc aaatcatagg ccgtgcgccg cacgaccact
    12721 tcgcggcgat gctcgacaaa ggcttccagg atccgcttga ggttcagcac ctccggccgg
    12781 ttgttgacca gcgccagcat gttgacgccg aaggtcgtct gcaactgact gtgtttatac
    12841 aaattgttca gcactacgag cggaatttca ttgcgcttca gttcgatcac gacccggacc
    12901 ccttcgcgat cggactcgtc ccggatatcg gaaatgccgg tcacgcggtc ttcctgcgcc
    12961 aggtcggcaa ttttttcgat cagcttcgac ttgttcacct ggtaggggat ttcagtgatg
    13021 atgagccgct cgcgatcggt gcgttcatcg gtctcgatcg ccaccttggc ccggaccgtg
    13081 agaagcccgc gaccggtctc ataggcatcc ttgatgccgg acgtgccgta gataaatccg
    13141 gcagtgggga agtccggtcc gggaattttt ttcatcagct gcgcaatcgt cacctccggg
    13201 ttctccagca gcaggagcaa cccctcaatg acttcgccca gattgtgggt gggaatgttg
    13261 gtcgcgtaac cgacggcaat cccgcccgcg ccgttgacca gcagattcgg cacccgcgag
    13321 ggcaacacca gcggctcaac tcgcgactcg tcgtagttgg ggccgaagtc gacggtctct
    13381 ttttcgatat ccgccagcaa ctcttcggcc agtttggtca accgggcttc ggtataccgc
    13441 atggccgctg ccgcgtcgcc gtccatcgaa ccatagttgc cctgaccgtc caccagcatg
    13501 tagcgcatgt tgaagttctg cgccatgcgc accagggtat cgtagatcgc cgagtcacca
    13561 tggggatggt aattacccat gatctcgccg acgatctttg ccgacttacg gtatggccga
    13621 ttggcggcca atcccatctc gttcatgcca tgcaaaatgc gacggtggac cggtttcaac
    13681 ccgtcccgca cgtcgggcaa cgcgcgaccg acgatcacgc tcatcgcgta gtcaaggtac
    13741 gacgagcgca tctcgtcttc gatcgcaatt tggcctaatc gttcatctgg cggcatctac
    13801 cctccacgga tgctgacaat gtctcttggc ttcgttctcg gctcgagaag gtcctcaacg
    13861 tacccctgag tctacgcgtc cggtcctctc ttgcctgcgg ccttgccgaa gcccattttt
    13921 gagcatcctt cgaattcaga aaaccaaagt ggttgtgtct acgatcacga ccgactatac
    13981 gtccaaattc cggacttcca gcgcatgttg ctggataaag ttacgccggg gctccacttc
    14041 atcgcccatg agaatcgtga aaatttcgtc gacgccggtc atgtcttcga ggttgacgcg
    14101 caacagcgtg cgcttctcgg gatccatcgt ggtttcccag agctggcccg gattcatttc
    14161 gccgagacct ttataccgtt gaatgttcaa gccctgtttg ccttcctcca ggatggcctg
    14221 taccagctcg gccgagccgt tcttgagaac ctcaccttcc ttgatcttga tcttgtacgg
    14281 aggtttcccc aacccgatcg aggagggagc ctgtttctgc aattcacgga agtcagccga
    14341 tccgaccagc tcgtgcgtca cctgcaactc ataggcgatt ccgccagtgg cgaccttaca
    14401 gaccagtttg ctcgattgat gctcttcatc ttccaggatc tcgatcgtcg gttcggcctt
    14461 cgggtagacc agtgccagtg tggccttcac gtgatcaacg accgtgcgca gcgacgccgg
    14521 atccttcaag acttctcgcg tcaacgccgg ttcatcgacg aacgcccgta gcatgttggc
    14581 ttcatgatgc ttcttgttga ccttatgcag aaggctttcg aaggcaatca gcttcttgag
    14641 aatcggcagc aaccgccggc ccgtgacgaa ctcgcggccg ttctcgagcg cgacttccac
    14701 cccctccacg gcaaggtcgg cgagatactc gttcatcgcc ggctcgtcct tcaggtaccg
    14761 ttccgtcttg cctttcttca ctttaaagag gggaggctgc gcgatgtaaa tgtagccccg
    14821 ctccaacagc tcaggcatct gccggaagaa gaaggtgagc aacagcgtgc ggatgtggct
    14881 gccgtccacg tcggcgtcgg tcatcagcac aatcttgtgg taccgcgtcc tggcgatgtc
    14941 gaacgcgtcc ttgtccgcct tgtcggaatc ttctttcttt cggccgatgc ccgttcccag
    15001 cgccagaatc agcgtgcgga tttcgtcgct gctcagcatc ttgtcgaacc gcgctttttc
    15061 cacattgagg atcttgccct tgagcgggag gatggcctgg aacttccggt cgcggccctg
    15121 tttggccgat ccaccggcgg aatctccctc gacaatgaac agttcgctca aggccggatc
    15181 tttttcggaa cagtcggcca gcttgccggg caaggatccg ccgtcgagag cgctttttcg
    15241 ccggatgaga tctttcgctt tccgcgcggc ctcgcgggcg cgcgccgcat cgatggcctt
    15301 gccgatgatt ttgcgagcga ccgggggatt ctcttcgaag taggtgccga gtgcgtcgtt
    15361 gactgccgcc tcgacaatac ccttcacttc gctattgccg agtttcgcct tggtctgtcc
    15421 ctcgaattgc gggttccgca ctt
//
LOCUS       DAOQEW010000084        15344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1069_length_15344_cov_7.643665, whole genome shotgun sequence.
ACCESSION   DAOQEW010000084 DAOQEW010000000
VERSION     DAOQEW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_15344_cov_7.643665"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PLS35_10420"
     CDS             complement(<1..889)
                     /locus_tag="PLS35_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNI19123.1"
                     /translation="MMHEPERLSEPVDAPLVPASVSLPEITPKAVLLSVLLAAVLAGA
                     NAYLGLFAGMTVSASIPAAVVSMAVLRLFRQSNILENNIVQTAASSGEALAAGVIFTI
                     PGLVLVGYWTSFDYWQTFTVSLVGGVLGVLFTIPLRRALIIHARLRFPEGVATAEVLK
                     IGATSGGNAGRRVRLLLSASALGGGLKFAEGGLKLWSESLEGALELGRMTFYGGLNLS
                     PALVAVGYIIGLNTALVVFLGGAIGWLVLLPLYQALVGTPADVTGVAAAKVVWSGQIR
                     YIGIGAMLVGGVWTLIQVRG"
     gene            complement(894..2090)
                     /locus_tag="PLS35_10425"
     CDS             complement(894..2090)
                     /locus_tag="PLS35_10425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNI19124.1"
                     /translation="MTQPMTGSTARVRLTAERAGPRYYGHLWVFDNNVAEVLGTPAAG
                     DLVDVYTHQKRFFGRGLFNPHSKIRIRLLTFHEEPIDEAFFAARLRAAAALRRTVTPH
                     ANACRLVHGESDLLPGLVVDRFADVAVMQALGYGMDLRKDLLGDLLVQEAGVKSVYLR
                     NDAKSRTLEGLPLSKGFLRGEGPTTVPIHEGKAQFTVDIAEGQKTGWFCDQRENRLAA
                     AAYAKGKRVLEAFCHTGAFGVQAALAGAQSVEGLDVSAAAVALAQAHAAQNHVASICT
                     YRQADAFDELRVLERNQQRYDLVILDPPAFARSKKALPHALAGYKDVNLRGLRLLQSG
                     GVLVSCSCSQPVSDDDFWMMLQSAARDANRQIRLLEQRGQGPDHPVLAGMPETRYLKC
                     FIVQVF"
     gene            2290..3942
                     /locus_tag="PLS35_10430"
     CDS             2290..3942
                     /locus_tag="PLS35_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNI19125.1"
                     /translation="MTTHTIGTAVLDRLHRLGVRHMFGIPGDYVLSLYKLMESSPIQH
                     VATTREDCAGFAADAYARINGIGALCVTYCVGGLNTVNAIACAYAERSPVVLLTGSPG
                     LSERARNPYLHHMVREFSTQREVFEKMTVAAVSLDDPLTAEREMDRAFAALLRYRRPI
                     YLEIPRDMVHVPLAYTSHKTSILDEPTDKAALSEAVTEVRVMLESAKRPVILAGAEVG
                     RFGLHDELTKLVERLNVPIASTLLGKSIIREDHPLYVGVYSGLVARDEVKEFVNQTDC
                     LLILGSILSDVEDLDARSALFSDGHTIHATADRVAIKHHRYDSIRFEDFVKALANAPL
                     PSFPKPQLPAPRAPEEPMPPADASVSLHGVFRHLDTVLQEKTLVIADVGESLFASVDL
                     HVHRRFEFLSPAYYTSMGFAVPAAVGASFADPSLRPIVLVGDGAFQMTGSELSTAVRY
                     RQAPVVIVLNNHGYSTEREILEGPFNDIHEWRYERICDLIGGGQGSRVATHGEFVDTL
                     AKALADPSQPYVINVLLDPSDRSPAMMRLARRLAKRLSTDRP"
     gene            4132..5094
                     /locus_tag="PLS35_10435"
     CDS             4132..5094
                     /locus_tag="PLS35_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19126.1"
                     /translation="MSPKMPDGKTMRDGQPFSSRLNNVVTTEREFDELVTKSLPELLD
                     RATGQTKRFLRETNQWGDDITHEKLALRWGYELVERFVVFGRTEVPCRPFFLLDSLIA
                     KSFSQPDPLCYHKELLTPVGRFLDGLASRAVVSRDALIALFYHFYGFSQAHVVKLLGF
                     GQAESQRVYKNFERWRQSGWQRTMNETGLSGPEIEILEQELRTRPDHLNNEVDRLMPV
                     LQSHYRKSEPEHYPCLSEQKWGQLFHDDYGHDYRVWHLAFCRDCFLEVADRRQADVSS
                     GVKPQVNLHIHPLKKGGVLALFGGDRGGRHHGNTRAQRLSRTSA"
     gene            5051..6352
                     /locus_tag="PLS35_10440"
     CDS             5051..6352
                     /locus_tag="PLS35_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19127.1"
                     /translation="METPELNDYRAHLLDALDDQPRRAAEERPGFLQVLVNHERIGTL
                     NCSQTDPSFAYTARERSIQHLELRSESGVLIGGCVAPEAGQKSVRIPVGKGSLTLHVQ
                     NHFDGGSLNVQYEVAPGWWSHLATAIGLPAGAAVASTQGSPVWSMTTAFAQVVLAVGV
                     VALLAERIPAWVGSEDRAHQLEEELAARQSTQDQIDHVHQQLAQLVESQAAAVAVSRA
                     EQDRIAQLNTLIDTVAHTQQRLSTQVVAVQEDLKTVKTDVAQEVQTGMRVAVTAVEAD
                     RELVRQDLQAVKSVNETLVKQVALLESRNRELHARLALTSLEVAKANAASKPVVVAKN
                     EPPKETPAPTAVAVSDNLRDADRQAFMFWVAFQDGTTEKSIEDLVQELNGIKKGPMKS
                     GWYSVEVNLPNPEPPDRFLESVKRAKIVKSVVTSKAMPPAQ"
     gene            6484..7716
                     /locus_tag="PLS35_10445"
     CDS             6484..7716
                     /locus_tag="PLS35_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNI19128.1"
                     /translation="MSDFHQNGLVTVLHRLGGSNLEQLEKELERHAASNPIALVLPSL
                     YSELENPALKHIVQVLKDIRYVNEIVISLDRASALEFRLAKQFFSVLPQRVRIVWNDG
                     AGIQDILKLLAQAELDTGLQGKGRGCWMAFGYVLARGESNVIALHDCDILSYNREYLA
                     RLCYPIVNTNLGYEFCKGYYSRVTNRLHGRVTRLYFTPLIRSLQQVAGAHPLLTFLDS
                     FRYPLAGEFAMVRDLAWINRVPGDWGLEVGVLSEIYRNCALRRICQADIADAYEHKHQ
                     GLSADNAEQGLQKMCVDITKSLFRNLASEGVVLSEGVLKTLRATYLQSAQEAITRYQN
                     DAAINSLQFDRHAERMAVEVFLKGMKIATEAFLEDPLGVPMISNWSRVTGAIPDVFER
                     LIGAVERDHDWDPVGDRV"
     gene            complement(7848..9983)
                     /gene="glgX"
                     /locus_tag="PLS35_10450"
     CDS             complement(7848..9983)
                     /gene="glgX"
                     /locus_tag="PLS35_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNI19129.1"
                     /translation="MTEVLEGRPTPRGATWDGQGTNFSLFSAHATKVELCLFDPTGEK
                     EIDRVSLPEYTDQIWHGYLAGIHPGTPYGYRVHGPYDPDAGHRFNPNKLLLDPYAYAH
                     MGELQWNPALFGYQMESGDDRTFDERDSAPFMPKCTVVDQNFDWSGPRDRRTIPWDDT
                     VVYEMHVRGFTKLHPAVPSTLQGTYEGLATKEVLDYLQSLGVTSIELLPIHTFVNDSH
                     LLEKGLTNYWGYNSIGFFAPDPRYAANRPESVRECKEMIARLHDAGFEVILDVVYNHT
                     AEGNELGPTLSFKGIDNSSYYRLQPEQPRYYINDTGTGNTLNLSHPRVIQLVTDSLRY
                     AVQQMHVDGFRFDLGTILAREPNGFDNRSGFLKVCSQDPLLATVKLIAEPWDCGPGGY
                     QVGGFPPGWAEWNDKFRDDVRDFWRGELAATNLADRLCASASVFNHQGRRPWSCVNFV
                     TAHDGFTLHDVVAFNEKHNEANGEDNQDGSSNNRSWNCGAEGPTEDASINALRDRQMR
                     NLMATLLISQGTPMVLAGDEFGRTQRGNNNAYCQDNEISWVDWSLLETRSSLFQFVKE
                     LIAFRHHYPILRRNLFLTGQLAEDSGVRDVTWINANGGPMEEHHWTDTTIHCFGMLLD
                     GRAPTTGRRQPGKDVTVLVLINGHHEPVQFTLPPCVGASEWTLLLDTNLPDRPQNQTF
                     QMGTQYLLTDRSLVLLVLGAKRTEEIIPV"
     gene            complement(10122..10289)
                     /locus_tag="PLS35_10455"
     CDS             complement(10122..10289)
                     /locus_tag="PLS35_10455"
                     /inference="COORDINATES: protein motif:HMM:NF023180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HNI19130.1"
                     /translation="MPNIVLVILILLLVGVLPTWPYSSGWGYYPSGGLGLLLLIVLVI
                     ALSGRRGALRS"
     gene            complement(10396..12462)
                     /locus_tag="PLS35_10460"
     CDS             complement(10396..12462)
                     /locus_tag="PLS35_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19131.1"
                     /translation="MVRTRLEERIIILAPIGQDAPAMARVLISQGVDAIVCATALDTC
                     RELAVGGAVLLVTEESLELPHISQLLHHLKAQPPWSELPMIVLTRGGESRLARLLDLV
                     AEAAGSITLLERPIGEATLMRSIEVGLRSRRRQYQVRDLLEQETLSQQQLRESEQQYR
                     TLFESIDQGFCTIEVLFDEQQEPVDYRFLLVNPAFAVQTGLRDVVGRHMRDIVPQHEE
                     HWFRIYGNIATTGEPLRFEKEAKALDRHYEIYAWRIGLPSEQKVAVLFNDITQRKASQ
                     FRLEQFAEELERKVSERTYELVASQQQLRALATELNLAEQRERTRLAAELHDHLAQML
                     VLVRLRLGQAKVGPLSHSIQMIKQAEDVVDESLTYTRNLVAELSPPVLHEFGLFVALR
                     WLGEQMRRYHLEVSVRIDTPEELKLPENQAVLVFQSVRELLLNAVKHAGSKKAWIAAA
                     SEDGVLSIVVEDRGVGFDPITLSSQKLAAMSSKFGLFSIRQRMEALGGRLELRSKLGE
                     GTKATLVLPITQLSEQATGISLSYIAPSAHVARAPSTGHCKSVPYRVLVADDHAMVRQ
                     GLCLMLDACPDIEVIAEAANGQEALELARRLKPSVVVIDINMPLMNGIEATAQIRTAL
                     PDTIVIGMSVNASVDNQEAMRTAGASVLLPKEAAGDQLYGAIQSAMKGLSEHTGPLIR
                     SQETRL"
     gene            complement(12449..13963)
                     /gene="gvpD"
                     /locus_tag="PLS35_10465"
     CDS             complement(12449..13963)
                     /gene="gvpD"
                     /locus_tag="PLS35_10465"
                     /inference="COORDINATES: protein motif:HMM:NF018756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD"
                     /protein_id="HNI19132.1"
                     /translation="MTDPTRQADSRCETGIDGLNEILGDGLPANCMYLVQGDPGSGKT
                     TLALQFLLEGVRLGEKTLYITLSETKEELVTVARSHGWSLDSISLFELSAIDSLVRPE
                     ARTTVFHPSEVELTKVSEMLQSEIRRIQPARVVFDSLSEFRLMAETALRYRRQLINLK
                     QELAKYNCTALLLDDRMDNSRIGGDPHVLSLTHGVIEMEQLSPDYGRSRRRLRVLKMR
                     GMKFREGYHDYSIEKGGLRVFPRILASDHRADFSPQAVSSGNQEFDELLGGGLDRGTT
                     TLIMGPAGTGKSTLAMQYAAQMAQRNESSVLFAFDEIRGIVLARATAVGLDVERHINT
                     GLIDVQQVDPAELSPGEFGMRIREAVEGGCTLLIIDSLNGYLNAMPGEKYLVNQLHEM
                     SAYLNQKGVVTILILAQYGLIGQAEAPLNLSYLADTVVSVRFFEAAGAVRQALSVIKK
                     RSGGHEKTIREYQLVSDGGLRIGPPLKQFQGVLTGTPSFYGALEQVMERPDGAN"
     gene            14368..15123
                     /locus_tag="PLS35_10470"
     CDS             14368..15123
                     /locus_tag="PLS35_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19133.1"
                     /translation="MPNWLAYAGAILTGLIPPLIMYLIGSQKKVLGYALTQNEPMINL
                     RHPDLDGRLSMSLDGKNLNGCRIMGLVIQNLGMKDIEEQSVHLTFPAGVTVLHAHCSS
                     SALAIGPKIEARQADSHEVRIPLMNPGDKLMVRLLMTGNEAGRILVTAKGPSLKFKPF
                     DPTTFISPAVNRGLLVMSGAIFAWIVWACAGTDRFMARPFWDKTITLALIGLFLAIPS
                     AILAYQGTIRLLKRALQWREGNRASSLQPHPEL"
BASE COUNT         3346 a         4616 c         4245 g         3137 t
ORIGIN      
        1 gccctcgaac ttgaatcagc gtccagactc cgccgacgag catggcgccg atcccgatgt
       61 agcgaatctg cccgctccac acgaccttcg ccgccgccac accggtcaca tcagccggcg
      121 tccccacaag ggcttggtac aagggcagga gcaccagcca gccgattgct ccgccgagaa
      181 aaaccaccag cgccgtattg agcccgatga tgtaccccac tgcgaccagc gccggcgaga
      241 ggttgaggcc tccgtagaac gtcatacgac ccagctcaag cgcaccctcc aacgattcac
      301 tccaaagttt taagccgccc tcggcaaact tcaacccgcc ccccaaggct gacgcgctca
      361 acaacaggcg aacccggcga ccggcattgc cgcctgatgt ggcgccgatc ttcaatacct
      421 ccgccgtggc aactccctca gggaaacgca gccgcgcgtg aatgatcaag gcacggcgta
      481 agggaatcgt gaacaagact cccagtactc cgccgaccaa cgacaccgta aaggtttgcc
      541 aatagtcgaa gctcgtccaa tagccgacca ggaccaggcc aggaatagta aaaatcacac
      601 ccgccgctaa tgcctcaccc gaagaggcgg cggtttgcac gatattgttt tctagaatgt
      661 tggactggcg aaacaggcgc agcaccgcca tcgagacgac agcggctggg attgacgcgg
      721 aaacggtcat ccccgcgaag aggcccaggt aggcattggc cccagccaag acggcagcca
      781 gtagaaccga gagcaggacg gctttgggag tgatttccgg cagcgacacg gaggcgggca
      841 caagcggcgc atcgacaggc tctgacagcc gttcaggctc gtgcatcatc gcctcagaag
      901 acctgcacga tgaaacattt cagataccgt gtctccggca tcccggccag caccggatgg
      961 tccggcccct gcccccgctg ttccagcaga cggatctggc gattggcatc ccgcgccgcc
     1021 gactggagca tcatccagaa atcgtcatca ctcaccggtt gcgaacagga acaactcacc
     1081 aggacgcccc ccgactgaag aaggcgcaag cctcgcagat tgacgtcttt atacccggcc
     1141 aacgcatgag gcagggcttt cttgctccgg gcaaaggcag gtggatcgag aatgaccaga
     1201 tcataccgtt gctgattgcg ttccagcact cgcaattcat cgaaggcatc ggcctgccga
     1261 taggtgcaga tggacgcgac atgattctgc gccgcatgcg cttgggctaa cgccaccgct
     1321 gccgcactca catccaggcc ttcgaccgat tgcgcaccgg ccaaggccgc ctgcactcca
     1381 aaggcgccgg tatgacagaa ggcctccaac acgcgttttc ctttggcata ggccgctgct
     1441 gccaggcggt tctcccgctg atcgcagaac cagccggttt tttgcccctc cgcaatgtcg
     1501 actgtgaact gagccttgcc ttcgtggatg ggcaccgtcg tcggtccctc gccacgcagg
     1561 aatcctttcg acaacggcag cccttcgagc gtccggcttt tcgcatcatt gcgcaaatag
     1621 acgctcttca ctcccgcttc ttgcaccagg aggtcaccca acaggtcctt tcgcagatcc
     1681 atgccatacc ccagggcctg catcaccgcg acatcggcaa accggtccac caccaggccc
     1741 ggcaacaggt cactctcgcc atggacaaga cgacacgcgt tcgcgtgagg ggtgacagta
     1801 cgccgaagcg ccgcagcagc acgaagtcgc gcggcaaaaa acgcctcatc gatcggttct
     1861 tcgtgaaagg tcagaagccg gatgcggatt tttgagtggg ggttgaacaa cccgcgtccg
     1921 aaaaaccgtt tctgatgggt gtacacatcg accaaatccc ccgcggccgg cgtgcccagc
     1981 acttcggcca cattgttgtc gaagacccag agatgaccgt agtaacgagg cccggcacgc
     2041 tcagcggtca ggcgaactct tgcggtagaa ccggtcatag gctgtgtcat ggtggtgatg
     2101 tatccagggt agaggtgttc aatggcatgt tggccatgcg gtgcaaatgg ctcacgaggg
     2161 tgcctgaaat cattccctga agcaaccgtg cgtcacgatt gttcgcgcgc ttgaccttcc
     2221 caggcaccta tggtttgctg cgagaataga tccctcgcct cgccacctgg taacggagcg
     2281 tccatcctta tgacgacaca cacgatcggc acggcagttt tagatcggct tcatcgcctc
     2341 ggcgtccgcc atatgttcgg cattcccggc gactatgtgc tgagcctcta taaattgatg
     2401 gagtcctcgc cgattcaaca tgtggccacc acgcgagaag attgcgccgg ctttgctgcc
     2461 gacgcctatg cgcggatcaa cggcatcggg gccctctgcg tgacctattg tgtcggagga
     2521 ctcaacacgg tcaatgcgat tgcctgcgcc tatgcggaac gatcacctgt cgtgttgctg
     2581 accggatcac caggactctc ggagcgcgcg cgcaatccct atctccacca tatggtgcgg
     2641 gagttctcga cccaacgtga ggtgtttgaa aaaatgacgg tggcggcggt cagcctggac
     2701 gatccgctga cggcggagcg cgaaatggat cgcgcgtttg ccgccttgct gcgctatcga
     2761 cgtccgatct atctcgagat cccgcgcgac atggtccatg tgccgctggc gtacacgtct
     2821 cataaaacga gcatcttaga cgaaccaaca gacaaggccg ccctaagtga agcggtgacc
     2881 gaggtgcgcg tgatgttgga gtcggccaaa cgtccggtga tcttagccgg cgctgaagtg
     2941 ggccgctttg gactccacga cgagctgacg aaactcgtcg aacggttgaa cgtgccgatc
     3001 gcctccaccc tcctcggaaa gtccatcatc cgcgaagacc atccgctcta tgtcggcgtc
     3061 tatagtggct tggtcgcccg tgacgaggtg aaggaatttg tgaaccaaac agattgcctg
     3121 ctcatcctcg gttcgatcct gtcagatgtt gaggatctcg acgcccgcag cgccctgttt
     3181 tccgatggcc ataccatcca cgccaccgcg gatcgcgttg ccatcaaaca tcatcggtac
     3241 gattccatcc gcttcgagga tttcgtcaaa gcacttgcca atgccccgct gccctccttt
     3301 cccaaaccac agctgcccgc tccccgtgct ccggaagaac ctatgcctcc ggcagacgcc
     3361 tcggtcagtc tgcacggcgt attccgccat ctcgatacgg tgctgcagga gaaaacactg
     3421 gtcatcgccg atgtcggcga atcgctcttt gcctccgtcg atctgcacgt ccaccggcgc
     3481 tttgagtttc tctcgccggc ctactatacg tcgatgggct tcgccgtccc ggcggccgtc
     3541 ggggcctcgt ttgccgatcc gtccttgcgc cctatcgttc tggtgggcga cggggcgttt
     3601 cagatgaccg gttcggaact ctccaccgcc gtccgatacc ggcaagcgcc tgtcgtgatc
     3661 gtcctgaata atcacggcta ttccaccgaa cgcgagattc tggaaggacc gtttaacgat
     3721 attcacgaat ggcgatacga acggatctgc gacctcattg gaggggggca gggctcgcgc
     3781 gtcgccactc atggtgagtt cgtcgatacg ctggcgaagg cactggccga tcccagccag
     3841 ccctatgtca tcaacgtctt actcgatccc tccgaccgtt ccccggccat gatgcgtctc
     3901 gccagacggc tggccaagcg tctctccacc gaccgtccgt agggggattc tcccgtcgaa
     3961 agccaccccg cccctgcgtg aggaaccgca ccgttttcca tctgcctcca agcctgtttc
     4021 accctgatac aatgcgggga tcgcccgcct cccccattga cccctcatgg acaaatgtgg
     4081 tctcctagga gcggacttgt cgaattgtca tttgctgggg aattcgccac tatgtctcct
     4141 aagatgccgg acggaaagac gatgcgcgac ggccaacctt tttcttcacg actcaacaat
     4201 gtcgtgacca ccgaacggga gttcgacgag ctggtcacga agtcgctgcc ggaattgttg
     4261 gaccgtgcca ccggtcagac caagcgcttc ctgcgtgaaa cgaatcaatg gggcgacgat
     4321 atcacccatg aaaaattggc gcttcgctgg ggttacgaat tagtggagcg ttttgtggtc
     4381 ttcggccgca ccgaagttcc ctgcaggccg ttttttctgt tggatagcct gatcgcgaaa
     4441 tcgttcagcc agccagatcc cctgtgctac cacaaggagt tgctgacgcc ggtggggcgg
     4501 tttctcgatg ggttggcttc ccgcgccgtc gtgagccgcg atgcgctgat cgcgctgttt
     4561 tatcacttct acgggttcag ccaggcccat gtggtgaagc tgctgggttt cggccaggcg
     4621 gaaagccagc gcgtctataa gaacttcgag cggtggcgcc aatcgggctg gcagcgcacg
     4681 atgaacgaaa cgggattgag tggcccggaa atcgaaattt tggagcaaga attgcgcacg
     4741 agaccggacc acctcaataa cgaagtggat cggctgatgc ccgtcttgca atcgcattat
     4801 cgaaagagcg aaccggaaca ttatccctgc ttgtccgaac agaagtgggg gcaacttttt
     4861 cacgatgact acggccacga ctaccgcgtc tggcacctgg cgttttgccg cgattgcttt
     4921 ctggaagtcg cagatcgccg gcaagccgac gtcagcagcg gggtgaagcc gcaagtcaat
     4981 ctccatatac atccgctgaa aaagggaggc gtcctggctc tctttggagg cgatcgggga
     5041 ggacggcacc atggaaacac cagagctcaa cgactatcgc gcacatctgc ttgacgcgct
     5101 ggatgaccag ccgcgccgcg cggcggaaga acgccctggg tttctccagg tcctggtcaa
     5161 tcacgagcgc atcggcacat tgaattgctc acagaccgat ccatccttcg cctatacagc
     5221 ccgcgaacgc agcattcaac acctggaact ccgaagcgag tcgggcgtgc tgatcggagg
     5281 gtgcgtggca cccgaagccg gccagaaaag cgtgcggatc ccggtcggca aaggcagcct
     5341 cacgctccat gttcaaaacc atttcgacgg cggttcgctc aacgtgcagt acgaggtcgc
     5401 gccgggttgg tggtcacatc tggccacagc cattggcttg cccgccggcg ccgccgtagc
     5461 ctcgacgcaa ggcagccccg tgtggagcat gaccaccgcc ttcgcgcagg tcgtcctggc
     5521 cgtgggcgtg gtcgctctgt tggctgaacg gattccggcc tgggtgggct ccgaagatcg
     5581 tgcccatcag ttggaagaag aattagccgc gcgacaatcg acgcaggatc agatcgatca
     5641 cgtccaccag caactcgccc agcttgtcga gtcgcaagcg gcagcggtgg ccgtgtcgcg
     5701 cgccgagcag gatcgcattg cgcaattaaa taccctcatc gacaccgtcg cccatacgca
     5761 gcagcgcctt agcacccaag tcgtcgccgt tcaggaagac ctcaagaccg tgaaaacgga
     5821 tgttgcgcag gaagtgcaaa ccggcatgcg cgtggccgtg actgcggtcg aagccgaccg
     5881 cgagctcgtg cgacaggatc ttcaagcggt gaagtcggtc aatgaaacgc tggtcaaaca
     5941 ggtcgcgttg ctggagagcc gcaatcggga gttgcatgcc cgtctggcct tgacctcatt
     6001 ggaagtcgcc aaagccaatg cggcatccaa gcccgtcgtc gtcgcgaaaa acgagccgcc
     6061 gaaggagacc cctgcgccga ccgcagtcgc ggtgtcggac aaccttcgcg acgcagatcg
     6121 ccaggccttc atgttctggg tggcttttca ggacggcacc accgaaaaaa gcattgaaga
     6181 tctggtccag gaacttaacg ggatcaagaa agggccgatg aagtcggggt ggtattccgt
     6241 cgaagtgaat ctgcccaatc ccgagcctcc ggatcgcttt ctggagtctg tgaaacgagc
     6301 caagatcgtg aagtcggtcg tgacgagcaa ggctatgccc ccggcgcagt aacggcgatc
     6361 tcctctcagc acattcaccc gctcggcacg gttggtgttt gcctccagtg atggaatcat
     6421 tccctaccgc accgggcata aacgaataca gccaccctct ccccgccccg cacaggaggg
     6481 accatgtcgg attttcatca gaacggtctc gtgaccgtgt tgcaccgcct cggcggctcc
     6541 aaccttgagc aattggaaaa ggagttggag cggcacgcgg catcgaaccc gatcgccctc
     6601 gtcctcccct ccctttattc cgaactggaa aaccctgcgc tgaaacatat cgtccaggtg
     6661 ttgaaagaca tccggtacgt caatgaaatc gtcatctcgc tggatcgtgc atcggcactg
     6721 gaattccggc tggccaaaca gtttttttcc gtgctgccgc aacgagtgcg aatcgtctgg
     6781 aacgacggag ccggtatcca ggacatcttg aaattgctgg cccaagccga actcgacacc
     6841 gggttgcagg gaaaaggccg gggatgctgg atggccttcg gatacgtcct ggcccggggc
     6901 gaaagcaatg tgatcgcgct gcacgactgc gacattctca gctacaaccg cgaatacctg
     6961 gccagactct gctacccgat cgtcaacacc aatctcggct acgaattctg caaaggttac
     7021 tacagccggg tgaccaatcg ccttcacggc cgcgtaacac gactgtattt cacgccgctg
     7081 attcgaagcc tccagcaagt ggccggggct caccctctgc tcaccttcct cgacagtttc
     7141 cgttacccgc tggcggggga atttgccatg gtgcgcgact tggcctggat caaccgtgtg
     7201 cccggcgatt gggggctgga agtcggcgtg ctgtctgaaa tctatcgcaa ttgcgcgctc
     7261 aggcggattt gccaggcgga tatcgccgac gcctacgaac acaaacacca gggtctgtcc
     7321 gcggataatg ccgaacaggg cttgcagaaa atgtgcgtgg atatcaccaa gtcgctgttc
     7381 cggaatctcg ccagcgaggg tgtggtgttg tcggagggcg tgctcaagac cttacgcgcc
     7441 acgtatcttc agtcggccca ggaagccatc acgcgatacc aaaacgacgc ggccatcaat
     7501 agcttgcagt tcgaccgcca cgctgagcgg atggccgtag aggtctttct gaaaggcatg
     7561 aagatcgcga ccgaagcatt tctggaagac ccgctcggcg tgcccatgat ttccaactgg
     7621 agccgcgtca ccggagccat cccggatgtc tttgaacgtc tcatcggcgc cgtcgagcga
     7681 gaccacgact gggatcctgt cggagaccga gtgtaagaga gatgtcctcc tgcgtggagg
     7741 cgattcacca cctgacgttc cttccccgac acacgaaact cttctgccgt cgcatcaggc
     7801 catttctctt acctgtgcct cgcatggatt gccgcgacgt gggcccgcta taccggaatg
     7861 atctcttccg ttcgcttggc tcctaatacc aataacacca gcgaacgatc cgtgagcagg
     7921 tattgggttc ccatctggaa ggtttggttc tgagggcgat ccggcagatt ggtatcaagc
     7981 agcagtgtcc attctgacgc accgacacaa gggggcaagg taaactggac cggctcatgg
     8041 tggccattga tcaggaccag taccgtgaca tccttccccg gttgtcgacg tccggttgtc
     8101 ggcgcccggc cgtccaagag cattccaaaa cagtggatcg tcgtatcggt ccagtgatgc
     8161 tcttccatcg gcccgccgtt ggcattgatc caggtcacat ctcgaacgcc ggaatcctcg
     8221 gccagctgtc cggtcaaaaa gagattgcgg cgtaaaatcg gataatgatg acggaacgca
     8281 atcaattctt tgacgaattg aaagagcgat gatcgtgttt cgagcaacga ccaatcgacc
     8341 caactaatct cgttgtcctg acagtaggca ttgttattac cccgttgggt gcggccaaat
     8401 tcatcgcccg ccaacaccat gggcgtccct tgagaaatca ataacgtcgc catgagattt
     8461 cgcatttgcc ggtcacgaag cgcgttgatt gaagcatctt ccgtcggtcc ctcggcgcca
     8521 caattccatg agcgattgtt agaggacccg tcctggttat cctccccgtt cgcctcgtta
     8581 tgcttctcgt tgaaggccac cacatcatgt agcgtaaagc cgtcgtgcgc cgtcacaaag
     8641 ttgacgcacg accagggccg ccggccttga tgattgaaga cggaggcgga tgcgcagagt
     8701 cgatccgcaa gattggtagc agcgagttcg ccgcgccaga aatcgcgcac atcatcccga
     8761 aatttatcgt tccactcagc ccaccccgga ggaaatcccc ccacttgata tccaccaggc
     8821 ccacagtccc agggctcggc aatcagcttc acggtagcca gcaaggggtc ctgactacag
     8881 actttcaaga agccgcttcg attatcaaat ccattcggct cgcgcgcaag aatggtcccg
     8941 agatcgaaac ggaatccatc cacgtgcatt tgttggacgg catagcgcag actatcggtg
     9001 actaattgaa tgacgcgggg atgactcaga ttcaaagtgt tccccgtccc tgtatcgtta
     9061 atgtaatagc gtggttgctc aggctggagg cggtaataac ttgagttgtc gatcccttta
     9121 aaggatagcg tgggaccgag ctcattgcct tcggcggtgt gattgtagac gacgtccaga
     9181 atcacctcga aaccggcatc atgcagccgc gcaatcatct ctttgcattc gcgaacactt
     9241 tccggtcgat tcgcggcata ccgcggatcc ggcgcgaaaa atccaatact gttatacccc
     9301 caataattgg tcagcccctt ctcaaggaga tgactgtcat tgacaaacgt atggatcggc
     9361 agaagctcta tagatgtgac ccccagcgac tgcagatagt ccagaacctc cttcgtcgcg
     9421 agcccctcgt aggtaccttg gagagtggac ggcacggccg gatgaagttt cgtgaaccca
     9481 cgcacatgca tttcgtacac aacggtgtca tcccacggaa tcgtacgccg atctcgaggt
     9541 cctgaccaat cgaagttttg atcgactacg gtgcacttcg gcataaatgg cgcactgtca
     9601 cgttcatcaa atgttcgatc atcgccggac tccatttgat agccaaacag agccggattc
     9661 cactgcagtt cgcccatgtg ggcataggcg tagggatcca gcagcagctt gttcggatta
     9721 aagcgatgac cggcgtcagg gtcataggga ccgtggacac gatagccata aggcgtccca
     9781 gggtggatcc cggccaggta tccatgccag atctgatcgg tgtattcagg caacgacacg
     9841 cggtcgattt ctttttctcc cgttgggtcg aagaggcaca actcaacttt cgtcgcgtgg
     9901 gcggaaaaca gactgaaatt ggtcccctgt ccgtcccatg tggcaccccg aggcgtgggg
     9961 cgcccttcga gcacttctgt cataatgatg aactcctaca tcctgctaac cgaaccattc
    10021 aggggccgtg ctccaaaagc aacgagattc ctctgccctc cgtcgaccgg cgctgattac
    10081 tcacagtccg gatgtatcca cgcgcaggca gcctgcttcg cctaacttct caacgctcct
    10141 cgtcgaccgc tcaaggcaat gacgagcacg atgaggagga gaagccccag tccgcctgaa
    10201 ggatagtacc cccacccact gctatacggc catgtcggca ggacacccac aagcaacaga
    10261 atgagaataa ccaggacgat gttaggcatg gaggactcct ttcagtgaga tgtctcacaa
    10321 ggtactggga aactaggtag ggagagacta ggaaaatccc ctggaaggag gtcaatgaac
    10381 ccaagaaccg ggagtctaca gccgtgtctc ctgtgatcgg atcaacgggc ccgtatgttc
    10441 ggatagcccc ttcatcgcag attgaatggc gccgtacagt tgatcaccgg cggcttcctt
    10501 cgggagcagg actgacgccc ccgcggttcg catggcctcc tgattgtcta ctgaagcatt
    10561 caccgacata ccaatgacga tggtgtccgg aagggcggtt cgtatctgcg cagtggcttc
    10621 gatcccgttc atcagcggca tattgatatc gatcaccacc accgacggct tcaatctccg
    10681 cgctaactcc agcgcttcct gcccattggc agcctcagca atcacctcaa tatccggaca
    10741 ggcatccagc atcagacaga gcccctgccg caccatggca tgatcatccg ccaccaggac
    10801 ccgataggga acggacttgc aatgaccggt gctgggtgcg cgcgcgacat gcgcggacgg
    10861 tgcaatgtag gacaatgaga tcccagtcgc ctgctccgaa agctgcgtga tcggcaacac
    10921 cagcgtggct ttggttcctt cgccgagttt tgaacgcagc tccaagcggc cgcccaatgc
    10981 ttccatacgt tggcgaatac tgaataggcc aaacttggac gacatcgcgg cgagtttttg
    11041 tgacgacaac gtgatgggat caaaccccac cccccgatcc tccacgacga tgctgagcac
    11101 tccatcttca ctggctgccg cgatccacgc tttcttggac ccggcgtgtt tcactgcgtt
    11161 gagaagtaac tctcgaaccg attgaaaaac gagcacggcc tgattctcgg gcaatttcaa
    11221 ttcttccggc gtgtcgatac ggacgctgac ctccaagtga taccggcgca tctgttcgcc
    11281 cagccagcgg agggcgacaa agagcccgaa ctcatgaagc acgggcggac tgagttccgc
    11341 cacgagattg cgcgtatagg tcagggattc gtcgacgacg tcttcagcct gtttgatcat
    11401 ttggatggaa tgagacaaag ggccgacttt ggcctggcct aatctcagcc gaaccagcac
    11461 cagcatttgc gccaaatgat catgaagctc ggcggccagc cgtgtgcgct cccgctgttc
    11521 ggccaaattg agctccgtgg ccaacgcccg gagctgttgt tgcgaagcca ccagttcata
    11581 cgtgcgctcg gagaccttgc gttcaagctc ctccgcgaat tgctccaagc ggaactgcga
    11641 agccttccgt tgcgtgatat cgttgaagag gaccgcgacc ttttgctcgg acggcaggcc
    11701 gatgcgccag gcatatatct cgtaatggcg atccagcgcc ttcgcctcct tctcgaaccg
    11761 caatggctca ccagtcgtgg cgatgttccc gtaaatccgg aaccaatgct cttcatgttg
    11821 tggcacgata tcccgcatgt ggcggcccac cacatcgcgg agccctgttt gcacggcaaa
    11881 cgccgggttg acgagcagga agcggtagtc gacaggctcc tgctgctcgt caaacagcac
    11941 ttctatcgtg cagaagccct gatcgatgga ttcgaacagc gtccgatatt gctgttcgga
    12001 ttcccgcagt tgctgttgcg acagtgtctc ttgctccaaa agatcacgca cctgatattg
    12061 acgccgccgc gatcgcaatc cgacttcgat ggagcgcatc aacgtcgcct cgccaatggg
    12121 ccgttcgagc aacgtaatgc tgccggctgc ctcggcgacc agatcgagga gacgtgccaa
    12181 gcgggattct ccgcctcgcg tcaacacaat catcggcaat tcgctccacg gcggctgcgc
    12241 cttgagatgg tggagcaact gggaaatatg aggcagctcg agggattctt ccgtcacgag
    12301 caggacggcg ccacccacag ccagctcacg acaggtgtcc agagccgttg cgcagacgat
    12361 cgcatccaca ccttgcgaga tgagcaccct tgccatggcc ggagcgtcct gaccgatggg
    12421 ggcgaggatg atgatgcgct cctcaagcct agttcgcacc atcagggcgc tccatgacct
    12481 gttcgagggc gccgtagaag gaaggggtcc ccgtcaggac accttgaaat tgcttcaggg
    12541 gtgggccgat acgcaacccg ccgtctgaca ccaattgata ttctctgatg gttttttcat
    12601 gccccccgct tcgcttcttg atcaccgaca acgcctgccg aactgcgccg gctgcctcaa
    12661 aaaatcgcac gctcacgacc gtgtcagcga gatagctcag atttaacgga gcctcggcct
    12721 gcccgatgag cccgtattgg gccaaaataa ggatggtgac gacgcctttc tggttgagat
    12781 aggcggacat ttcgtggagc tggttgacga gatacttttc accaggcatc gcattcaaat
    12841 atccattcag actgtcgatg atcaggaggg tacagccacc ctccacagcc tcgcggatgc
    12901 gcatgccgaa ttcgccggga gaaagttcgg cgggatccac ctgctgcaca tcgatgagac
    12961 ccgtgttgat atgccgctcg acatctaacc cgacagcagt cgcgcgcgcc agcacaatgc
    13021 ctcgaatttc atcgaatgca aacaggacgc tcgattcatt tcgttgcgcc atttgtgctg
    13081 catactgcat ggccagcgtg gactttcccg ttccggccgg acccatgatg agcgtggtgg
    13141 ttccacgatc gagcccgccc ccgagaagct cgtcaaactc ctggttgccg ctcgagaccg
    13201 cctggggcga aaaatcagcc cgatgatcgg aggcaagaat ccgggggaac actcgcaggc
    13261 cccccttctc aatactgtaa tcgtggtagc cttcgcggaa tttcattcca cgcatcttga
    13321 ggacacgcaa ccttcgccgg gaccggccgt aatcgggcga caactgctcc atttcgatca
    13381 cgccatgggt gaggctgagg acatggggat ctccaccgat ccggctattg tccatcctgt
    13441 catccagcag cagtgcagtg cagttgtatt tcgccagttc ttgtttgagg ttgatcagct
    13501 gccgccgata ccgtaacgcg gtttcagcca tcaagcgaaa ctccgacagc gagtcgaaca
    13561 ccacgcgcgc aggttgaata cgccggattt ccgactgcag catttcgctg actttggtga
    13621 gctcgacttc cgaagggtga aacaccgtcg tccgtgcctc aggccgtaca agcgaatcga
    13681 tggccgagag ttcgaagagc gaaatggagt ccagcgacca gccatgcgaa cgggcaaccg
    13741 tgaccagctc ttctttcgtc tcggagaggg tgatatagag ggttttttcc cccaagcgca
    13801 cgccttccag aagaaactgg agcgcaagcg tggtcttgcc cgagcccggg tctccctgca
    13861 ctaaatacat gcagttggcc ggtagtccgt cgccaaggat ttcgttcagt ccgtctatgc
    13921 ccgtttcaca gcgactatcg gcctgcctgg ttggatcagt catggtccag cctcaaaaga
    13981 gtgagtgatc aatcacggcc atatgaaaat tcttccgaaa atttatccgc agtatagcgt
    14041 ggagaaaaac cgagcggact agaacaatcc ctagtgcccc cctcgataag agatagtcgt
    14101 cgaacccgaa agcctagggt gttagtggct gaaggggtct tatgctggaa aatcgatcgg
    14161 gcatgacgca ccgcgcggag gatgcacaca tgctcgagat acggaggaca ggatcataga
    14221 aagaaaactc aactagcttc caggcaatta aaagaacggg gattaaccgc gaagaatgtt
    14281 ttcgaggcag acggtcctac caggggtctg aattgcggtt tccatggtgg aagctatact
    14341 tcctgtaaca cttgcgagga ggatgtgatg cccaattggc tcgcttatgc cggagcaatt
    14401 cttaccggtc tgataccacc gttgatcatg tatttgatcg gctcgcaaaa gaaggtttta
    14461 ggctatgccc ttacccaaaa cgaaccgatg atcaacctcc gacatccaga ccttgacggg
    14521 cgactctcaa tgtcactcga tggcaagaat ctgaacggat gtcggatcat ggggctggtc
    14581 attcaaaacc tggggatgaa ggatattgaa gagcaatcgg ttcacctcac atttcccgcc
    14641 ggagtcaccg tcctgcatgc gcactgttcg tcgtctgctt tggcgatcgg gcctaaaatc
    14701 gaggcccgcc aggctgattc gcatgaggtc cgcattcccc tcatgaaccc aggggacaaa
    14761 ctgatggttc gtctcttgat gacgggaaat gaagccggtc gtattctggt gacggccaag
    14821 ggaccaagcc tcaaattcaa gccattcgat cccacgacat tcatttcgcc agcggtgaat
    14881 cgtgggcttc tagtcatgag cggggctatc tttgcttgga ttgtctgggc ctgtgctgga
    14941 accgatcgat tcatggccag gccattttgg gataaaacga ttacgcttgc gctcattggc
    15001 ttgttcttgg ccattccctc agcgatcttg gcttaccaag ggacgattcg cctgttaaag
    15061 cgggcgctgc aatggaggga aggtaacagg gcgagctcgc ttcaacccca cccagagctc
    15121 tgatcgtaag tcagcggcgc ttcattttgc gaagccgcac gaaaaaaaac gacagtgatt
    15181 aatggatatc ggccgcaaac catgtcttgt caagaagctg ggggaagggg cttctcgacg
    15241 tctgagaacc taccctttcg ccttctgcgc gaataccgtc gcatcacagg cgatgtactt
    15301 aatctgcttc atcggcacaa tgatgacttc cttggccgaa tcca
//
LOCUS       DAOQEW010000085        14879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1121_length_14879_cov_8.349366, whole genome shotgun sequence.
ACCESSION   DAOQEW010000085 DAOQEW010000000
VERSION     DAOQEW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_14879_cov_8.349366"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLS35_10475"
     CDS             <1..918
                     /locus_tag="PLS35_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNI19134.1"
                     /translation="ELAFTLYNGLTYVEAAVKAGLEVDSFAPQLSFFFNVHNDFFEEI
                     AKFRAARRLWAREMERRYHPKNPRSLQLRCHAQTAGCSLTAQQPMNNVVRTTLQALAA
                     VLGGTQSLHTNSMDETLALPTEEAVKLALRTQQIIAAESETVNSVDPLGGSYVVESLT
                     NRLEEGALDLFRKLDGLGGMVSAIERGFPQREILDASQRYQREIERQERSIVGVTTHR
                     EEDVRSIPILKIGPEVEQEQVARLSDLRKSRDPFKMAGSLEELQEMAACHQNLMPSLI
                     EAVKAKATLGEICAALKEVYGTYREPVVL"
     gene            915..1757
                     /locus_tag="PLS35_10480"
     CDS             915..1757
                     /locus_tag="PLS35_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI19135.1"
                     /translation="MKLGAFDIYPVTDGRFRLDGGAMFGVVPKVLWQTCCPADDLNRI
                     PLSLTCLLIRAHGKHMLVDTGLGDKENEKFRSMFAVERKPTLRESLSQHGLCRDDIHL
                     VVNTHLHFDHAGGNTATDGRSAVAPTFPKATYYVQQTEYDDAMQANERTRASYRRDNF
                     TPVAEANQWALLKGETELLPGVTAIVTPGHTRGHQSVKVESEGRVAFFLGDLIPTVSH
                     LPLPYIMGYDLDPVQTLDSKRWVLERACEEHWLLLFEHDPYVQAGYVSKTVDGKYALQ
                     EVTL"
     gene            1754..2161
                     /locus_tag="PLS35_10485"
     CDS             1754..2161
                     /locus_tag="PLS35_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNI19136.1"
                     /translation="MTVAATPLRILIGKVGLDGHDRGVKLVARALRDAGIEIIYTGLH
                     QSPEQIVTTAIQEDVQAIGLSIHSGAHNSLFPRILGLLRERGAEDIVVFGGGIIPDED
                     VPRLKAAGVQMLFRPATPMQDIVEFVNGLRTER"
     gene            2175..3779
                     /locus_tag="PLS35_10490"
     CDS             2175..3779
                     /locus_tag="PLS35_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNI19137.1"
                     /translation="MRVLDTSIRRDSDEFRRNQAHYEGLMADLQKHLEMVWAGGSREA
                     IALHKQRGKLTARERISSLLDPGSPWLELSPLAAFGLYDNQVPSAGLITGIGSVSGRF
                     CVIAANDATVKGGTYFPMTITKHLRAQTIALENRLPAIYLVDSGGVFLPMQAEVFADK
                     EHFGRIFFNQARMSAAGLPQIAVVMGMCTAGGAYVPAMCDENVIVQGTGTIYLAGPPL
                     VKAATGEEVTAEALGGADLHTRLSGVSDHLADDDRDALDICRSIIATLPRRLPKPRRT
                     HIDEPLYPAEDLYGLIPANPRQKWDVREVIARLVDGSRFHEFKARYGATLVCGFAQWM
                     GHMVGIVANNGVLLSESALKGAHFVQLCAQRRVPLVFLQNITGFMVGKDYESRGIIKD
                     GAKMVQAVSTAEVPKFTILIGASHGAGNYAMCGRAYGPRFLFSWPNARISVMGAQQAA
                     QVLVTVKEQQRAREKAALTDDERRRIIEATQRQYEQEGSPYFSSARLWDDGILDPTDT
                     RRVMGLCLDLAALAPVKDSHAPVFRM"
     gene            3799..4644
                     /locus_tag="PLS35_10495"
     CDS             3799..4644
                     /locus_tag="PLS35_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNI19138.1"
                     /translation="MRPFTTLMIESNGAIARVTLNRPDRRNAFDGQMATELREAFEDL
                     AHDPSIRGIVLAAAGSVFCAGADLQWMQSDGPASETPAMRDAHHLLRMFRAIDEFPCP
                     VIARVQGSAFGGGLGLLAVCDIVVATEEATFALSEPRLGLIPAVVAPFLLHKAGESFL
                     RRYGLTGETFCASTAQRYNLAHDVVPQSNLDDRINELSDALMQLAPCATKESKLLFHR
                     LRALSDKERLALGPEYNARARCAPEAAEGLRAFIEKRPPAWAQPRERKPQEATTSDDV
                     VLRHR"
     gene            4622..5569
                     /locus_tag="PLS35_10500"
     CDS             4622..5569
                     /locus_tag="PLS35_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNI19139.1"
                     /translation="MLCFATDKTRRLPVRIIEVSPRDGLQHEAIFVPTARKIDLINAL
                     SHSGVTEIEAGSFVSPTAIPQLADSDEVFRAIDRLPDVTYSALVPNERGLERARAAAV
                     QKIAVFTAASNSFTRHNIKATVQESLVRFTPVIHGAKRDGMLVRGYISTALWCPYEGR
                     IAPKLTVDVMRRLLDLGVDDISLGDTIGRASPADIRGLLDAVMPHIEPTRLSLHVHDT
                     YGMAVANVLTAWEDYAITAFDCSAGGLGGCPYAPGASGNVATEDVVFALKASGATVAV
                     DEELIVDCARQLSAVIGHPLHSRLSQISRPRIGQPALKV"
     gene            5571..6389
                     /gene="meaB"
                     /locus_tag="PLS35_10505"
     CDS             5571..6389
                     /gene="meaB"
                     /locus_tag="PLS35_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNI19140.1"
                     /translation="MNHVTAHPSFFRHDAAELARQVEAGEVRAVARVISLLENRDPLG
                     AAVLTHVAPSLNRALVIGITGYPGTGKSTVIDQLITAYRRLGEKVGVLAVDISSPVTG
                     GAVLGDRIRMQRHTDDARVYIRSMATRGHQGGLAAATRQAVRVLDAAGFGVILIETVG
                     VGQSDVDIMQVAHTVVAVVAPGLGDDVQAMKAGLLEVADIVVVNKGDREGADAALRDL
                     REWCKTVIRTVAVKGEGIAELIAVIAEHERWRDLDRPALHNRRGPSIDLGSRTG"
     gene            6421..7815
                     /locus_tag="PLS35_10510"
     CDS             6421..7815
                     /locus_tag="PLS35_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HNI19141.1"
                     /translation="MLADRLAHYTQSLRFKDLPGDVVHEVKRRILDSLGCAFGAWTAT
                     PCRIAREMALAVKVPGGATVWGTNHKTLPDLATFANGAMVRYLDFNDTYLSKEPAHPS
                     DNIAAVLAAGETAHASGQLIIQALTLSYEIQCRLCDAAALRPRGWDHVTYGPLSSALG
                     IANVLKLTDAQTRQAVNLAGVANVALRQTRVGDLSMWKACAFSNAARNGMFAALLARR
                     GMTGPAPIFEGEKGFMKLVSGPFELPMLGGEAMPGGEPVPFKILKTCIKHFPVEYHAQ
                     SAVEAALALRAELLATEGKTWINKVADVEIGSYDVAIEIIGRDPEKWQPATRETADHS
                     FPYCVAVALLDGRVSMNSFSPKRLRDPRLHELMKKIHVVKLADLEHRYPTTMPTRLTI
                     RTSQGSTHSRQVDQPLGHPGHPLSDQEVGDKLRRLTSRRIDRAQLDRLLAFVWHLEDQ
                     TDIAAFMPLLRVRS"
     gene            7812..8750
                     /gene="prpB"
                     /locus_tag="PLS35_10515"
     CDS             7812..8750
                     /gene="prpB"
                     /locus_tag="PLS35_10515"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNI19142.1"
                     /translation="MTFDHSSGDKGRRLQEIVARRTVAIPGASNALTAVQIERAGFDA
                     VYVSGAAVSAARGLPDIGLISLSEMVGEAGIIARAVAIPTIVDADTGYGPPSTVMNAV
                     REFERAGIAGMQIEDQEATKKCGHLEGKRLIPLGDMVAKISAAVKARHNRNFLIVART
                     DARTVDGLDGAIQRAKAYAEAGADMLFPEALLSPEEFGTFSLAIRNAGIHLPLIANMT
                     EFGRTPYLSVDEFQALGYQAVLFPVSSLRVAARAVEKLLSELKFFGSQRNWLDHMMTR
                     QELYDLIHYEDGTTSMRRSHEPDHAGTVNGEGSRPS"
     gene            8698..9876
                     /locus_tag="PLS35_10520"
     CDS             8698..9876
                     /locus_tag="PLS35_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNI19143.1"
                     /translation="MNQTMQEQSTVKAHDHPEYSPGLEGVIAGESAICQVDEGEAGLR
                     YRGYAIGDLAGRSSFEEVAYLLLFGHLPATTELEEFSRLLVRNRRLPQQVHHFVESLT
                     PDMHPMDVLRTGISLLGLHDPDAQDGSREANLRKSVRLLAQIPHLVADSYQIMADRQP
                     ATPVSHGSFAENLLSLVAGRRYGEIGAAMAEALNVSLILYAEHEFNASTFAARVTAST
                     LTDLHGAITAAVATLKGPLHGGANEAVAAMLIEIGRPEHVEFWLRDQLAHKRRVMGFG
                     HRVLRQGDARSAIIQHHAERLSDLCGDRNWYHIATTLDRLMLEEKGLRPNLDFYTAVA
                     YLLMGIPRGLSTPLFVCSRITGWCAHVIEQQEHNRLMRPRALYTGPAPKAYEPLERRT
                     "
     gene            9851..11569
                     /locus_tag="PLS35_10525"
     CDS             9851..11569
                     /locus_tag="PLS35_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HNI19144.1"
                     /translation="MNLLNDVRDHIEQARDVSGRATDVPWASFAEFLRSRVYDPQLAT
                     THALTFCDDDRALRRTYTYAELGAIIERLAGVFHTRFGLTRGDRVATLLFNHDVTVLT
                     YLAAWTLGVAVVPINIEETPEKKRYILEQSEVSTVFCWQDGYEELCALQSDIPTLRQV
                     IALGDAGVLDNHRTGKKFHAHGAGRSLRRSPFTSGLDDHALIVYTSGTTGPPKGVILT
                     VANLLIDADAIASWHSFGLHDRLMCVLPIHHVNGIVVTLITPLYCKGSVVMNRRFKTT
                     SFWRRIHEEQVTSVSVVPTLLEFLLDADEDLTPYDLSRLGGLICGAGPLLKDTAARFE
                     HRFGVVIRHGYGLSETTCYSCFLPNDLTQEQHRHWLTGYELPSVGVPVRHNLMTVLDE
                     RGRTLPPATRGEICIRGRTVCAGYFKRDDANEAAFQWGWFRSGDEGFYLLDDSGRPFF
                     FVSGRLKELIIRGGVNISPLEIDDVLKTHQAVKFAMALPFENRYYGEEIAAYVVPKDP
                     ASIPSEADVLAHCRMRLPFSKQPKVVLFGDDVPYTSTGKPKRLELKARLAGRLAQYRD
                     TQFRDQ"
     gene            11596..13602
                     /locus_tag="PLS35_10530"
     CDS             11596..13602
                     /locus_tag="PLS35_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNI19145.1"
                     /translation="MSTLIKGFERIEEARERLTGASFLAGLFDGRPDFEFLLTPPEPP
                     EERAAGMAFCKQVETFLRQEVDPDEIEREAKIPDAVIRGLFKLGAFGMKIPKAYGGLG
                     FSYTNYGRVLTLIASWSNILALTVAVPQSIGIAMPILLFGNEDQKRRYLPLVATEALS
                     AFALTEPMTGSDAANVRTEATLDANGSHFLVNGEKLWCTNGPIARYATLIARVPARRV
                     VREGQTTWMPVPEGQGADDRVHTAFILDMSTPGMQVRQRCQFEGCRGIENAHMTMQEV
                     RIPVENVIGEVGKGLKYALTILNVGRGISIPAICLGMAKQAWQPTLDRANGRVTFHKP
                     LAERQTQQIRLGDMAAHLFAMEALSMLVWRLADQHRHDIRIEAAVAKIFCSEHTIQFL
                     RDAQILFGGMGYETAASKMARGEAGFGIEQLVRDAEMYRIGEGATDILRPFIVREGLS
                     HHLDRAKPFYSGDLSILDQLKQLVKLGGFYGPWYLRHWVRHSLPTGSAFAHPQVRSVA
                     RYVERTSRRLAREIFYAMVRFQAAFQDEQRVQNRIESIGEDLLAMIATVLYAEQQTRL
                     EGRTTVWELAEAFHVAARKRITQRLREIRRHCDGVTAETGQQALKGYYPGLSDGIVHR
                     RLDDYRRKQAVSEAMPLAMASVSLDVKDRSLFAPVKAKAGERST"
     gene            complement(13615..14544)
                     /locus_tag="PLS35_10535"
     CDS             complement(13615..14544)
                     /locus_tag="PLS35_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNI19146.1"
                     /translation="MMTGISLDTILAWLITMSAAVGIASLRIGLVLAIGYVAIRFVRL
                     GLHQLERVMIVASDKQDQESGTAAKRAATLTGILRTIALSAIWAIIIIESLQLVGLDI
                     APILAGAGILGLAVGFGAQNLVRDLISGFFIILEDQVRLGDVAVINGTGGAVETITFR
                     TISLRDFSGVVHIFPNGGITTLSNMTKDWSAFVLDMGVAYREDTDRVVEVMRTVGEGL
                     RQDQAFGSLIIEPIEIVGVENFADSAVTIRARIKTKPAEQWKIGREYRRRLKKAFDAQ
                     GIDIPFPMRTLLMGETDQPLKVEVIAGLGLGKS"
     gene            14805..14877
                     /locus_tag="PLS35_10540"
     tRNA            14805..14877
                     /locus_tag="PLS35_10540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14838..14840,aa:Met,seq:cat)
BASE COUNT         3002 a         4503 c         4527 g         2847 t
ORIGIN      
        1 gaactcgcct tcacgctcta taacgggctg acgtacgtcg aggcggccgt gaaggccggt
       61 ctcgaggtgg attcgtttgc accgcagctc tcctttttct tcaacgtcca taatgatttc
      121 ttcgaagaga ttgccaaatt ccgcgcagcc cggcgcctct gggcccgcga gatggaacgg
      181 cggtaccatc caaagaaccc ccgttcactc caactccgct gtcatgccca aaccgcaggg
      241 tgctccctca ccgcccaaca gccgatgaac aacgtggtgc ggaccacgct gcaggccctc
      301 gccgccgtgc tcggcggcac acaatcactt cacacgaatt ccatggatga aacgctggcg
      361 ctgccgacgg aagaagccgt gaaactggcg ttacgcacgc aacagatcat tgcggccgag
      421 agcgagacgg tcaattcggt cgatccattg ggcggatcat atgttgtcga gtcgctaacg
      481 aaccgcctgg aggaaggagc gctggacctc tttcgcaaac tggacggact gggcggcatg
      541 gtctccgcca tcgagcgcgg gtttccacag cgggagattc tcgatgcctc gcagcgatat
      601 cagcgtgaga tcgagcggca ggaacggagc atcgtcgggg tgacgacgca tagagaagaa
      661 gacgttcgat ccattcccat cctgaagatc gggccggaag tcgaacagga acaggtggcc
      721 cgactgtccg atctcagaaa atcacgcgac cccttcaaga tggccggctc gctggaagag
      781 ctgcaggaaa tggccgcctg tcaccagaac ctcatgccct cattgatcga agccgtgaag
      841 gcgaaggcga cgttgggtga gatttgtgcc gccttgaagg aagtgtatgg aacctatcgg
      901 gaaccggtgg tcttatgaaa ctcggcgcct ttgacatcta cccggtaacc gacggccgat
      961 tccggctgga cggcggcgcc atgttcgggg ttgtcccgaa agtgctgtgg caaacctgtt
     1021 gcccggcgga tgacctgaac cgcattccgc tcagcctgac ctgcctgctg atccgggccc
     1081 acggcaagca tatgctggtg gatacggggc tcggcgataa ggagaatgag aaattccgct
     1141 cgatgtttgc ggtcgaacga aagcccactc tccgagagtc cctctcgcaa catggcctct
     1201 gccgcgatga tatccatctg gtggtgaata cgcatctgca cttcgaccac gcgggaggca
     1261 atacggcaac cgatggacgc agcgcagtgg cgccgacgtt tcccaaggcc acctattatg
     1321 tccagcagac agaatacgac gatgcgatgc aggccaacga acggaccagg gccagttatc
     1381 gccgggacaa tttcacgccg gtggcggagg ccaaccagtg ggctttgctc aagggtgaga
     1441 cggaactcct gccaggagtg acggccattg tcacacccgg gcatacgcgc ggccatcaaa
     1501 gtgtcaaagt ggaatccgaa ggccgggtcg cgtttttcct cggcgatctc attcccaccg
     1561 tgtcccatct tccgcttccc tacatcatgg ggtacgacct cgaccctgtt cagacgctcg
     1621 acagcaaacg atgggtgctg gagcgtgcct gcgaggaaca ttggttgttg ctcttcgagc
     1681 acgatccgta cgtgcaggcc ggatacgtca gcaaaaccgt ggacgggaaa tatgctctcc
     1741 aggaggtgac gctatgaccg tcgcggcaac acccttgcgc attctgatcg gcaaggtggg
     1801 attggacggc catgaccggg gtgtaaagct cgtcgcccgc gcgcttcgtg atgcgggcat
     1861 cgagatcatt tacacagggc ttcaccagag ccctgaacag atcgtgacga ccgcaattca
     1921 ggaagatgtg caggccatcg gtctcagcat ccattccggc gcacataatt ccttgttccc
     1981 ccgcatactc ggactgctgc gggagcgagg cgcagaagac atcgtggtct tcggtggagg
     2041 gatcataccc gacgaggatg tgccgcgatt gaaggcggca ggcgtgcaga tgttgttcag
     2101 gcccgccaca ccgatgcagg acatcgtgga atttgtgaac gggcttcgaa ccgaacgctg
     2161 agaggattcg agccatgcgt gtgttggaca cgtccatacg acgagactcg gacgagtttc
     2221 gccggaacca ggcccattac gaaggcttga tggccgatct gcagaagcat ctggagatgg
     2281 tgtgggccgg cggatccagg gaggcgatcg cgctccacaa gcagcgcggc aaactcaccg
     2341 cccgcgaacg gatttcatca ctcctcgatc cgggttctcc ctggctggaa ctgagtcccc
     2401 ttgccgcgtt cggcctctat gacaatcagg tgccgtcagc cggactcatt accggcatcg
     2461 gttcggtgtc cggccggttc tgtgtcatcg cagcgaacga tgcgaccgtc aaaggcggca
     2521 cctacttccc gatgacgatc accaaacatc ttcgcgcgca aaccatcgcg ttggagaacc
     2581 ggctgcccgc catctacctg gtagattccg gcggcgtatt cctcccgatg caggccgagg
     2641 tctttgccga caaggaacat ttcggccgga tcttcttcaa tcaggcgcgc atgtccgcgg
     2701 cgggtcttcc tcaaatcgcc gtggtcatgg gcatgtgcac ggccggcggc gcttacgttc
     2761 cggcgatgtg tgatgagaat gtcatcgtcc aggggaccgg caccatttat ttggccggcc
     2821 caccactggt caaggcggca accggcgagg aggtcaccgc cgaggcattg ggcggggctg
     2881 atctgcacac acgactctcc ggtgtgagcg accatttggc cgatgacgat cgtgacgcgt
     2941 tggacatctg ccgttccatc attgccacat tgcctcggcg actccccaag ccgcggcgga
     3001 cccacatcga cgagccactg tatccagcag aggatctcta tggactcatc cccgccaacc
     3061 cgcggcaaaa gtgggacgtc cgtgaggtga ttgcccggct cgtggacggc agccgcttcc
     3121 acgaattcaa ggcgcgatat ggcgctacgc ttgtctgcgg ctttgcgcag tggatgggcc
     3181 atatggtggg gatcgtcgcc aacaacggcg tgctgctttc agagtcggcg ctgaaaggcg
     3241 cgcatttcgt ccagctttgc gcccagcgtc gcgtgccgct cgtgttcttg caaaacatca
     3301 ccggcttcat ggtcggaaag gactatgaga gccggggcat catcaaagac ggggccaaga
     3361 tggtccaggc ggtgtcgact gcggaagtgc ccaagttcac gatcctgatc ggcgcctccc
     3421 atggggccgg caactacgcg atgtgcgggc gagcgtacgg cccgagattt ctgttctcct
     3481 ggcccaacgc gcgcatctcg gtgatgggcg cgcaacaggc agcccaagtg ctcgtcacgg
     3541 tgaaggaaca acaacgagcg cgagagaagg ccgctttaac ggatgacgaa cgacgccgca
     3601 tcatcgaggc cacccagcgg caatatgaac aggaaggcag cccttacttc agcagcgccc
     3661 gcttgtggga tgacggcatt ttggatccaa ccgacacgcg ccgcgtgatg ggactctgcc
     3721 tggatctcgc cgcattggcg cccgtgaagg acagccatgc gccggtgttt cgtatgtgag
     3781 cagccagagg aggtgcgaat gcggccattc accacgctca tgattgaatc gaatggcgcc
     3841 attgcgcgtg tgacgctcaa tcgacccgac cgtcggaatg ccttcgacgg ccagatggcg
     3901 acggaacttc gggaagcctt tgaagacctg gcgcacgatc catcgatcag agggatcgtg
     3961 ctggccgctg ccggctccgt cttttgtgcg ggagccgacc ttcaatggat gcagtccgac
     4021 gggccggcgt cggaaactcc ggcgatgcgg gacgcccatc acctgctgcg catgtttcgc
     4081 gccatcgatg agtttccttg tccggtcatc gcccgggtgc agggatcggc attcggaggc
     4141 ggacttggtc tcttggccgt ctgcgacatc gtcgtggcga ccgaggaggc cacctttgcc
     4201 ttgagcgaac cccggcttgg gttgattccg gccgtcgtcg cgccgtttct cttacacaaa
     4261 gcgggcgaat cgtttctccg acggtacggc ctcaccggcg aaacgttctg cgcatcgacg
     4321 gcccagcgat acaacctggc ccatgacgtc gtcccacaga gcaatctgga tgaccgtatc
     4381 aatgaattga gcgacgcgct catgcagctg gcgccctgtg ccaccaagga aagtaaactg
     4441 ttgtttcacc ggctgcgggc cctatccgat aaagaacgcc tggccctggg gccggaatac
     4501 aatgcccgcg cccgttgtgc tccggaagca gccgaagggc tgcgcgcctt tatcgaaaaa
     4561 cggccgcctg cgtgggccca gccacgtgag cggaagccgc aggaggcgac gacgtcggac
     4621 gatgttgtgc ttcgccacag ataagacacg ccggttgcct gtccggatca ttgaggtctc
     4681 acctcgtgac ggactgcaac acgaagcgat cttcgtgccg acggcgcgga agatcgacct
     4741 gatcaatgcc ctgtcacaca gcggtgtgac agaaatcgaa gccggttcct ttgtttcgcc
     4801 gaccgccatt ccgcaactgg ccgactccga tgaagtgttc cgcgctatcg accggcttcc
     4861 ggatgtgacc tattccgccc tggtgccgaa cgaacggggc ctggaacggg cacgggcggc
     4921 cgcagtgcag aagatcgccg tcttcacggc cgcctcgaac agtttcacgc gccataacat
     4981 caaggcaact gtccaggaat ccctggttcg ctttaccccc gtgatccatg gagcgaaacg
     5041 ggacgggatg ttggtgcgag gctatatctc caccgccctg tggtgcccct atgaggggcg
     5101 catagcgccc aaactgactg tggacgtcat gcggcggttg ctcgatctcg gtgtggatga
     5161 catctctctg ggagacacta tcggcagagc gtcgccggca gatatccgcg gcttgctgga
     5221 tgccgtcatg ccgcatatcg aaccgacccg cctgtcgctc catgtccatg acacctacgg
     5281 aatggccgtg gccaatgtgc tgacggcctg ggaagactat gcgatcacgg cgttcgattg
     5341 ttcagccggc ggtctgggcg gttgtcccta tgcgcccggt gcgtccggca acgtcgcgac
     5401 ggaagatgtg gtgtttgccc tgaaggcctc cggggcgacg gtcgcggtcg acgaagaact
     5461 gatcgtcgac tgtgcgcgtc agttgagcgc agtgatcgga catcccctcc actcgcggct
     5521 gtcacagata tcgcggcccc gtatcgggca accggccctg aaggtgtgat gtgaaccacg
     5581 tcactgcgca tccatccttc ttccgacacg atgccgccga gctggcgaga caggtcgaag
     5641 ccggggaggt tcgagccgtg gcgcgtgtca tcagtttgct ggaaaaccgg gatccgctcg
     5701 gagcggcggt gctgactcat gtggctccgt ctctgaatcg cgccttggtg attgggatca
     5761 ccgggtatcc cggcaccgga aaaagtacgg tgatcgatca gctcatcacc gcctaccgcc
     5821 gactgggaga gaaggtcggc gtgctggcgg tggatatcag cagccccgtc accggcggcg
     5881 cggtcctcgg cgatcgcatc agaatgcagc gccatacgga tgatgcccgg gtctacatcc
     5941 gcagcatggc gacacgcggg caccagggcg ggctcgccgc cgccacgcgg caggcagtac
     6001 gggtcttgga cgccgccggc tttggcgtga ttctgatcga gacggtgggc gtcggccaaa
     6061 gcgacgtcga catcatgcag gtcgcccata cggtggtcgc agtagtcgcg ccggggctcg
     6121 gcgacgatgt tcaggccatg aaggcgggac tgttggaggt cgcggacatc gtcgtggtga
     6181 acaagggcga tcgcgaaggg gcggatgcag ccctgcggga tttacgtgaa tggtgcaaga
     6241 cggtgattcg caccgtggcc gtgaaggggg aagggatcgc ggaactgatt gcggtcatcg
     6301 ccgaacacga acggtggcga gatctggaca ggccggcact ccataatcgg agagggccct
     6361 ccatcgacct cggctcgcga acgggataac gctgctgctg tcattgccat gaggtggtgc
     6421 atgctggccg atcgactggc tcactatacg caatcgctcc ggttcaagga tctacccggc
     6481 gacgtggtcc atgaggtcaa gcgccgcatt ctggacagtc tgggctgcgc attcggtgca
     6541 tggactgcca ccccctgccg gatcgcgcgg gagatggcgc tcgcagtgaa ggtccccggc
     6601 ggggcgacgg tgtggggaac gaatcacaag acccttcccg acctcgcgac cttcgccaat
     6661 ggcgccatgg tccgctacct cgacttcaac gatacctatc tgtccaaaga accggcgcat
     6721 ccctcagaca atatcgcggc agtcctagct gcgggggaaa cagcgcatgc ctccggccag
     6781 ctcatcattc aggcgctcac cctgtcctat gaaatccaat gtcggctctg cgacgcagcg
     6841 gccttgcggc cacgaggctg ggaccatgtc acttatggcc ccctctcgtc cgcactcggc
     6901 attgccaacg tcctcaagct gacggacgcg cagacccgcc aggccgtcaa cctggccggc
     6961 gtggccaatg tcgctctgcg ccagacacgg gtcggcgatc tgtcgatgtg gaaagcctgc
     7021 gcattctcca acgccgcacg caacggcatg ttcgccgccc tgctcgcccg gcgcggcatg
     7081 accggccccg cccccatttt cgaaggcgag aaagggttca tgaagctggt ctccggccca
     7141 ttcgaactgc cgatgttggg cggggaagcg atgccgggcg gagagccggt gcccttcaaa
     7201 attctgaaga cctgcatcaa acacttcccc gtggaatacc acgcacaaag cgccgtcgaa
     7261 gccgcattgg cgttgcgagc ggagctgctg gcgacagaag gaaagacctg gatcaataag
     7321 gtagccgatg tcgagatcgg cagctacgac gtcgccatcg aaattatcgg acgcgatccg
     7381 gaaaagtggc agcccgcgac gcgggaaacg gccgaccaca gctttccgta ctgcgtggcg
     7441 gtcgctctcc tcgacggccg cgtaagtatg aactcattca gcccgaaacg gctgcgagac
     7501 ccgcgtctcc acgagctgat gaagaaaatc cacgtcgtga aactggcgga tctcgaacat
     7561 cgctacccga ctaccatgcc gactcgcctc acgatcagaa cgtcacaagg ctcgacgcat
     7621 agccgtcagg tggatcaacc actcgggcat ccaggccatc ctctgtcgga tcaggaagtg
     7681 ggggacaaac tccggcggct cacgtccagg cgcattgatc gcgcgcaact ggatcgcttg
     7741 ctcgccttcg tctggcacct cgaggaccag acagatatcg ccgctttcat gccgctgttg
     7801 agggtgcgat catgacgttc gatcactcga gcggcgacaa aggacggcga ttgcaagaga
     7861 tagtggccag gcgcacagtg gccatcccgg gagcgtccaa tgcattgacc gccgttcaga
     7921 ttgaacgagc gggatttgac gcggtctacg tatccggcgc cgccgtgtct gctgcgcgcg
     7981 gcctgccgga catcggcctg atttccttgt cggaaatggt tggcgaagca ggaatcatag
     8041 ccagagccgt agccattcca accattgtcg atgccgacac cgggtatggg cctccttcga
     8101 ccgtgatgaa tgcagtccgt gaattcgagc gcgccggcat cgccggcatg cagatcgaag
     8161 atcaggaggc gacaaaaaaa tgcggccatc tcgagggcaa acgcctcatt cccctcggcg
     8221 acatggtcgc caaaattagc gccgctgtga aggccaggca caatcggaac tttctgatcg
     8281 tggcgcgcac cgatgcgcgg accgtcgatg gactcgatgg cgcgatccaa cgggccaagg
     8341 cctatgccga ggccggcgca gatatgttgt tcccggaagc gctgctatca ccggaggagt
     8401 ttggaacctt ttcgctggcg atacgaaacg cgggcattca tctcccgctg attgcgaaca
     8461 tgacggagtt cggccgaacg ccctatctca gcgtggacga gttccaagcc ctgggctatc
     8521 aggcagtgct gtttcccgtc agctcgttac gggtcgcggc gcgcgcggtc gaaaaactcc
     8581 tatcggagct gaagttcttt ggctcccagc ggaattggct cgaccacatg atgaccaggc
     8641 aggaactgta cgacctgatt cactacgagg atggcaccac atccatgcgg aggtcacatg
     8701 aaccagacca tgcaggaaca gtcaacggtg aaggctcacg accatcctga atacagcccg
     8761 ggcctggagg gcgtgattgc cggggaatcg gcgatctgtc aggtcgatga aggagaagcg
     8821 gggctgcggt atcgcgggta tgccatcggc gacctcgccg gacgaagcag cttcgaggag
     8881 gtcgcctacc tgctgctctt cggccacctc cctgccacaa cggaattgga ggaattctct
     8941 cgcctcctgg ttcgcaatcg tcggcttcct cagcaggtgc accacttcgt ggagagtctc
     9001 acgcccgaca tgcatccgat ggatgtgttg cgaaccggga tttcactgct cgggctgcat
     9061 gatcctgatg cgcaagacgg atcccgcgag gcgaaccttc gcaaatcggt gcgattgctc
     9121 gcgcagatcc ctcacctggt cgccgacagc taccagatca tggcggaccg gcaacccgcc
     9181 acgccggttt ctcacggaag ctttgccgaa aaccttctga gtctggttgc agggcgccga
     9241 tatggggaaa tcggggccgc catggcagag gcgctgaacg tctcgctgat tctgtatgcg
     9301 gaacatgagt tcaatgcctc cacctttgcc gcccgggtca ccgcgtccac actcaccgac
     9361 ctgcatggcg ccatcacggc ggcagtggct acgctgaaag gtccactcca tggaggcgcc
     9421 aatgaagcgg tggccgcgat gttgatcgag atcggccggc cggagcatgt cgagttctgg
     9481 ctgcgagacc agctggcgca caagcgccgc gtgatgggat tcggccaccg cgtgctccgg
     9541 caaggcgatg cgcggtcggc catcatccaa caccacgccg agcgattgag cgatctctgc
     9601 ggcgatcgca actggtacca catcgccacc accctcgatc gactcatgtt ggaggaaaag
     9661 ggactgcgcc cgaatctcga tttttataca gccgtggcct atctcctcat gggcatccct
     9721 cgcggactat ccacgccgct gtttgtctgt tcacggatca ctggctggtg cgcacatgtc
     9781 atcgagcagc aggagcacaa ccggttgatg agaccacggg cgctctatac gggccccgcg
     9841 ccgaaagcct atgaacctct tgaacgacgt acgtgaccat atcgagcagg ctcgcgacgt
     9901 atctggacgc gcaacggatg tgccttgggc gtcgttcgcg gaattccttc gttcccgcgt
     9961 ctacgacccg cagctcgcca caacgcatgc actgacgttc tgtgacgacg atcgtgctct
    10021 gcgccgaacc tacacctatg ccgaactggg agcgatcatc gaacggctgg ccggagtctt
    10081 ccataccaga ttcggcctga cccgtggaga ccgggtcgcc acgttgctct tcaaccacga
    10141 tgtgacggtg ctcacctacc tcgcagcctg gacgctggga gtcgcagtcg tcccgatcaa
    10201 catcgaagaa acgccggaga aaaagcgata cattctcgaa caatcagagg tctcaacggt
    10261 cttctgctgg caggacggct atgaagaact ctgcgcactc cagtcggaca tccccacgtt
    10321 gcgccaggtc attgcgctgg gtgatgccgg tgtgctcgat aatcacagga ccggaaaaaa
    10381 atttcacgca cacggagcag gccgctcgct tcgccgctct ccgttcacgt cagggctcga
    10441 cgatcatgcg ctgatcgtct acacctccgg cacgaccggc ccaccgaaag gcgtcatcct
    10501 gacggtggcc aatcttctga tcgatgcgga tgcgatcgcg agctggcaca gcttcggtct
    10561 ccatgatcgc ctgatgtgcg tcctgcccat ccatcatgtg aacggtatcg tcgtcacgct
    10621 gatcacgccg ttgtactgca agggcagtgt ggtgatgaac cgcagattca agaccaccag
    10681 cttctggcga cggattcatg aagagcaggt cacctcggtc agtgtcgtcc ccacgctgct
    10741 cgaatttctg ctcgatgccg atgaggatct caccccctac gatctgagcc gcctcggcgg
    10801 gttgatctgc ggcgccgggc ctctcttgaa agatacggcc gcgcgtttcg aacatcgctt
    10861 cggcgtcgtc attcgccatg gttatggcct gtccgaaacc acctgttact cctgtttcct
    10921 gccgaacgat ctgacgcagg aacagcaccg tcactggctc accggctatg agcttccctc
    10981 cgtgggagtg ccggttcgtc acaacctcat gacggtcctc gacgaacgtg gacgcacgct
    11041 gcccccggcc acccgcggag aaatttgtat tcgcggacgc accgtctgtg ccggctattt
    11101 taagcgcgat gacgccaacg aagccgcctt ccaatgggga tggttccggt caggagatga
    11161 gggattctat ctcctcgatg acagcggacg gccgttcttc ttcgtgtcgg gacgattgaa
    11221 ggaactcatc attcgaggag gggtgaacat ttctccgctg gaaatcgatg acgtgctgaa
    11281 gacgcaccag gccgtgaagt ttgcgatggc cctgccgttc gagaaccgct actacggcga
    11341 ggaaatcgcg gcctacgtgg tgcccaaaga tcccgcctcc atcccgagcg aagctgatgt
    11401 gctggcgcat tgccggatgc ggttgccttt ttccaagcaa cctaaggtcg tactgttcgg
    11461 tgatgatgtg ccctacacct cgacaggcaa acccaagcgg ctggagttga aagcgaggtt
    11521 ggcaggaagg ctggcgcagt atcgcgacac acagttcaga gatcagtgag ttgaacaggc
    11581 cgtgtgaggt cattcatgag caccctcatc aaaggattcg aacggatcga ggaagcgcgg
    11641 gagcgattga ccggggcaag ttttctcgcc ggactctttg acggccggcc ggacttcgag
    11701 ttcctgctga cgccccccga accaccggag gaacgggcgg ccggcatggc cttctgtaaa
    11761 caggtcgaaa cattcctacg gcaggaggtg gatccggacg aaatcgagcg tgaggcgaaa
    11821 attcccgacg cggtgattcg gggcttgttc aaactaggcg ccttcgggat gaaaattccg
    11881 aaggcctatg gcgggctggg cttctcctat acaaactatg gccgcgtcct tacgttgatt
    11941 gcgagttgga gcaacattct cgccttgacc gtggccgtgc cgcagtccat cggcatcgcc
    12001 atgccgatcc tcttgttcgg caacgaagac cagaagcgga ggtatcttcc gctggtggcg
    12061 acagaagcgc tctccgcctt cgcgctcacg gaaccaatga ccgggtccga tgccgcgaat
    12121 gtccggacgg aagcgacgct cgatgcgaat ggcagccact tcctcgtgaa cggcgaaaaa
    12181 ctctggtgca ccaatggacc gatcgcgcgt tatgccacgc tcatcgcccg tgtcccggct
    12241 cgacgagtgg ttcgagaggg acagacgacc tggatgccgg tgcctgaagg acagggagcc
    12301 gacgaccggg tgcatacggc gtttatcctg gatatgtcca cgccgggcat gcaggtccgt
    12361 caacgctgcc agttcgaagg gtgccgggga attgaaaacg cccacatgac gatgcaggag
    12421 gtccggattc cggtcgagaa cgtgatcggc gaggtaggga aagggttgaa atatgccctg
    12481 acgattttaa acgtggggcg cggcatcagc atcccggcca tttgtctggg catggcgaag
    12541 caggcctggc agccgacttt ggatcgggcc aacggtcggg tgacgtttca caagccgctg
    12601 gcagaacgtc aaacccagca gattcgcctg ggcgacatgg ctgcgcattt gtttgccatg
    12661 gaagcgctct ccatgctggt gtggcgactg gcggatcagc atcgtcatga catccgtatc
    12721 gaggcggccg tcgcgaagat tttctgctcc gagcatacga ttcaatttct ccgggacgca
    12781 caaattctgt tcggcggcat ggggtatgaa accgcggcct ccaaaatggc gcgcggcgaa
    12841 gcgggattcg ggatcgaaca gctcgtgcgg gacgcggaga tgtatcgcat cggtgaggga
    12901 gccacggaca tcctgcgccc ctttatcgtg cgtgaagggc tgagtcacca tctcgaccgc
    12961 gcgaagccgt tctattccgg cgacctgtcg attctcgacc aactcaagca gctcgtgaaa
    13021 ctggggggat tttatgggcc ctggtacctg cggcactggg tacggcactc cctgccgacg
    13081 ggctcggcct ttgcgcatcc gcaagtccgg tccgttgccc ggtatgtcga acgaaccagc
    13141 cggcgcctgg cgcgggaaat tttttatgcg atggtgcgct tccaggctgc gtttcaagat
    13201 gagcagcggg tgcagaaccg gatcgaatcc atcggagaag acctgttggc catgatcgcc
    13261 acggtgctgt atgccgagca gcaaaccaga ttggagggac gcacgacggt atgggagctc
    13321 gcagaggcct ttcatgtcgc ggcacggaaa cggattacgc aacgccttcg tgaaatccgg
    13381 cgccattgcg acggcgtcac cgcggagacc ggacaacaag cgctgaaggg atattatcct
    13441 ggcctgtcgg acggcatcgt gcatcgacgt ttagatgatt accgtcgcaa gcaggccgta
    13501 tcagaagcga tgccactggc catggcatcc gtgtccctag acgtgaagga ccggtctctc
    13561 ttcgcgcctg tcaaggcgaa ggcaggcgag agatccacgt gatcgctcac tgagctaact
    13621 ttttccaagc cccaggccgg cgatcacctc gaccttcaag ggctggtcgg tttctcccat
    13681 caacagggtg cgcatgggga agggaatgtc gatcccctga gcatcgaagg ccttcttgag
    13741 acggcgtcgg tattcgcgcc cgattttcca ttgttcagcc ggtttcgttt tgattcgtgc
    13801 gcgaatggtc acggcgctat cggcaaaatt ttcgacgccc acgatttcaa tcggctcgat
    13861 gatcagcgaa ccgaacgcct gatcctggcg caacccttct cccactgtcc gcatcacttc
    13921 gacgacccgg tccgtgtcct cccgataggc cacgcccata tccagaacaa aggccgacca
    13981 gtctttcgtc atattggaca acgtcgtgat gccgccgttg gggaaaatgt gcaccacgcc
    14041 ggagaagtcg cgtagcgaga tggtgcggaa ggtaatcgtt tccaccgccc cgccggtccc
    14101 gttgatcaca gccacatctc ccagccggac ctgatcctcc agaatgatga agaagccgct
    14161 gatcagatcg cgcaccagat tctgcgcgcc gaagccgacg gcgaggccca gaatgccggc
    14221 gccggccaag atcggggcga tatccaatcc aaccagctgc agcgattcaa tgatgatgat
    14281 cgcccaaatt gccgacaggg cgatggtccg gagaattccc gtcaacgtgg ctgcacgctt
    14341 ggcggcggtt ccggattcct ggtcctgctt atcgctggcc acaatcatca ctcgttcgag
    14401 ctgatgcagg ccaaggcgca cgaaccggat tgccacatac ccgatcgcca ggaccaaccc
    14461 gatgcgcaaa ctggcgatcc ccacggccgc ggacatcgtg atcaaccagg ccaggatcgt
    14521 gtccaatgag attccggtca tcatgcaggc ctccaattta tgagcgcgac cggcgcgctt
    14581 ctgcgatcac agggagacgg agagtttcgg ccgaatgccg cgcgacgctg caaatggcaa
    14641 gaagacgagc cggaacattc ggcttagcgt cataccatcc cccgacgatt ctgtccaagg
    14701 cccaattcct agccttccag gttttcgtca ggcaatcggt agacacttcg ggggcatttc
    14761 agttattatc cgcgttctct tcttcacacg accgatcgtg ctgtggcggt gtagctcagt
    14821 tggtagagca gcggactcat aagccgcggg tcacccgttc aatccgggtc accgccacc
//
LOCUS       DAOQEW010000086        14769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1141_length_14769_cov_9.099769, whole genome shotgun sequence.
ACCESSION   DAOQEW010000086 DAOQEW010000000
VERSION     DAOQEW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14769
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_14769_cov_9.099769"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLS35_10545"
     CDS             <1..832
                     /locus_tag="PLS35_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19147.1"
                     /translation="EAIGFVGDVYGAELGKEDLLRYGRYILVRGSVADLSPHLTEEEK
                     KAQHGGRITAGLRVRLASQGVTGVVYLEADYLDAQQYPTLDVPWKPQAVYLPSAPSTV
                     SVLGAALHTIARDLEKADIHTITRNLDSLVLEVTKLVKDTNEQALSSRTGRVLAEFQE
                     TARQTRRLVESPEIRAMVSDAAGTVQGARQLIADLSEASKQIKIASEQFPGVFARLER
                     TVQRIDRLVSNKSQDIEDTVENLRVMSENLREITDNAKRYPAQVLLGEPPPRAGATKR
                     "
     gene            879..1457
                     /locus_tag="PLS35_10550"
     CDS             879..1457
                     /locus_tag="PLS35_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HNI19148.1"
                     /translation="MFVLGGCVNLSKSYPEKHSYILEAVHSGEMRASIPGTVLKVRKF
                     RVSPTSEGKELVYRTSDARYEGDFYNEWFVPPNAMLTQQIMNWLTSAGLFQYVMDSSG
                     PLPATHMLEGTLTALHGDYRATPARAVLAVQFLFLHEASAQGEVLWHREYRKEVDIME
                     QKPEALVSGWNEALRLILSALDEDLTRTLRRQ"
     gene            1875..3239
                     /locus_tag="PLS35_10555"
     CDS             1875..3239
                     /locus_tag="PLS35_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI19149.1"
                     /translation="MSLVSLLRATFQGLRDDERTQGTLEVGEQLSSKRNVRWLWTGEL
                     ISQLGDGLNRPAILWFVYDLTGSAVKMTFIGVLQTIPPLVLGPVIGVYLDRLSADWKL
                     RVMISLDLARGALLAWLATLSILGMLTLPTLYTFVFLIALAAMPYGPALNSTIPSLVQ
                     ASRLTAANAAIQSTATLGLLCGPAISGATVAFLGVGYVLYANAATFLLAAFSKMPIRV
                     NSEAPSPSRSGTSSGWINELAAGFRLVFVEQRIIFRVALLGALFTLASTGFIFLLPII
                     TKNILSGGPMFLGVIWSALGLGMIFMSVLLMSSARRTLIDHLLLISAGPVIEGLAIVG
                     LAAVDSLVAALGLVIVIGAGTALVMPMITAFIQATAPQETRARALTIFGTITMVSAMA
                     GMTAFSWAADHLGSRIALVVLGVVQMMAGVIGIMFTQSSEFQTNLASRSNDHPSSKSD
                     TGIT"
     gene            complement(3501..3878)
                     /locus_tag="PLS35_10560"
     CDS             complement(3501..3878)
                     /locus_tag="PLS35_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNI19150.1"
                     /translation="MGECSLGSILVAKSERGICALFLGDDPDALARELQDQFPRATLI
                     GGDADFDQLVAKIVGFVEAPSLGLDLPLDVRSTAFQGRVWQALRRIPAGLRMSYREIA
                     EHLARKCLWKLIVSGRHRGMGRD"
     gene            3910..4287
                     /locus_tag="PLS35_10565"
     CDS             3910..4287
                     /locus_tag="PLS35_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNI19151.1"
                     /translation="MTKFAVGRERPNEGRGTHSFDPFHEFDRSFPSSHTARAFAVASV
                     FADRYEQPIPLIAYGTAAFISVSRVYLNEHFASDVVAGAVLGFVIGKVLSWRHKDENR
                     RWTVLPYVPDARGGLGMTFSCSF"
     gene            4374..5738
                     /locus_tag="PLS35_10570"
     CDS             4374..5738
                     /locus_tag="PLS35_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNI19152.1"
                     /translation="MARILSALLLSVLCAINGCTSAQVTQTHASQPNAFQAEGKKQEQ
                     SQEARSPEERLRKEKPQEERDEEDTVLSAIAAAPEYLVKGLAYPFKKFGIYYERTDLM
                     NRLMDLFYNDERTGGVFPRMAVGGALSSGIGFTAFHQDLFHQKKEVRARYLYATRGNQ
                     AADFAYRDPSLFGSKFMLDLDVFWLNFDNGFFFPGGNRAAENGKTKYDLNQLSQNARL
                     SYMVAPNLKASLLGRILFTDAGPSSRTPTTPPTVPGIDTYMQGAAVEPSLTYDSRDNP
                     FRPSTGLYIDTGFTYTNQLNGDQFRYMGYWVDVQRYIPVFRGDRVLVIRAYLSKLDSL
                     DGKSIPFYELNLLDLNNGLRGFDRGRWQDKGALLYNLEWRYPVWKDVEGTIFLDLGQV
                     FGDYKDLQPKLFQYSAGGGFRFVTSRSFAFRVQLAASNDGLLALFRGDLEFVRHRGTV
                     LGGF"
     gene            5798..7588
                     /locus_tag="PLS35_10575"
     CDS             5798..7588
                     /locus_tag="PLS35_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19153.1"
                     /translation="MRDWNWRRRISAFSCDGVAAWFLCALALSVIGCYNRVVLQEPVV
                     WEVNDRRPISEPQEETIGQWALWDGTDKMVFYRIGQLFNLGRSMRDLGAWVGLADPIE
                     AQNVNAFDEVPNSTWFTNRQFFSRRSEEELTAGPTTDRGAPDTAGPWTAIAAKASLGS
                     TPGFVIRDQKNDVYLIKFDPPLYPEMTTASEMITPRFLHAAGYNVPEHYLVYFDPAKI
                     RIDPKAKFRGKYHVPRPMQEQDITAILDRIPHRPDGTIRAMASKYLADVPKGPFLYEG
                     KRPDDPNDRILHENRRELRGFRILAAFVNHTDTKAANALDTYDPETRYLTHHLIDFSS
                     TLGADNADPQFPRFGNEYFFDFGTVGHSTIAVGSYVRPWEIPLEMHYPAVGYFESLYF
                     DPPRWRPTYPNPAFLRMTLRDAYWGAKIVTSFTDEDIDAIVHTGRYTDARAERYVADV
                     LKARRDKIGRYYFNLINPLDRFAIDTKFDEQVLVFENLALSLGYAPAPHALYRYSISK
                     YVPNWFDTSVIQETLVQSPKIRLDKAFLEAWGKHTADGRATEEERPIAAVRIQTSYDD
                     GSHWGKRVVVYLREHPGTRRMTIVGLERET"
     gene            7610..10378
                     /locus_tag="PLS35_10580"
     CDS             7610..10378
                     /locus_tag="PLS35_10580"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNI19154.1"
                     /translation="MNFVPILQKLVDIRSGEYRKTVLMFLYIFLLLLSFVIIKAVRDA
                     LFLVRLGPEQLPYIYILVAVIVGVVAWIHAKLSARLRLDVLIRGTLLIVVANLLIFWW
                     LFRFGWPSLYYVFYIWAAAIGLLTMSQYWLLANSVFNAREAKRLFGLIGAGSILGGLV
                     GGVVTARAVTWIGTEGLLIIAATGYLLCGGLALIVGRAAVPAARPSGNASGMSAQAEA
                     AAHSEESILTTMRNSPYLITIAGLIGIGEIVVMLVDYQFKVIATQLFPSKNDLTGFMG
                     IFSALVSASSFLVQVFFTGRILRSLGLGLALLCLPVGLLFGSVAIAVVPGLISAVMAK
                     LTEGSLRFSINKASLELLYLPLPWAIKAKTKPFIDPASERVAGGIGGLILIVATSILS
                     LSTAQISFVTIGLLSLWVPLALAGQRQYVDSLRLALERRRLDLTFPILNLSDRRTVDL
                     LIRHLLDSDDAGTCYVLRLLEQADVKPYIPAFKQLLTSRSPEVRAAVIRLLSEIREPS
                     LVPALTDVLGDQEMEILVGAVRFLDAHPGSSGAKLEEWMASTDPRLAAAAIVGLLNDP
                     AQRLGARAEQALRSMIQAHGPQGEIRRREAAKALGWIHPEATASDALIRLLQDDSIEV
                     VITAIKSAGRIQRREFVSPLVALLVRKETKGEAKTALVGYGSKILGGLADLLEDDNER
                     MELRRTLPSLLAEIGGERAIAILLHNLNQPDRLLRYQVVKALNRIRAHDPNLEPEPEL
                     IDASLTSELRHHYELLLLFHSPSLVEDSQGGRLLKKALQERLDHQLEMVFRLLALRFP
                     VSDIRDAYAGLTVDDARLRAIATEFMDNLLPSHLKRYLIPIIDNWPSDAVVEVGRQFV
                     SINGSTREELILYLLKGTDLWLKTCAIYLAGEWKLSSLLSELRQAADDSGHLIRETVH
                     LALEQLND"
     gene            10384..10800
                     /locus_tag="PLS35_10585"
     CDS             10384..10800
                     /locus_tag="PLS35_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNI19155.1"
                     /translation="MLTTIEKVLLLQGVDILVDTETENLAKIAAIAREVRFEEGATIF
                     EEGEPSNAFYIVLEGTVSVRKGSKEIGLVKDKETVGWVNLLEQTPRTVTLVALTGTTF
                     LKIEREGLFDLLADHFDIVRGILKRLSRIINQLLDR"
     gene            11258..11662
                     /locus_tag="PLS35_10590"
     CDS             11258..11662
                     /locus_tag="PLS35_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19156.1"
                     /translation="MTESIVVTGRLSEGTDEFHSPHGLDGPDHFYTFALAQRTRMEIA
                     VAANTSHWSDAVGLTRAAWQPALYLLSADGAMLKEGHVFRAGVTSLFPAEFEPGTYYL
                     VVDSSIREWSRGDGYYRLYVGFNEEVMGPLVP"
     gene            11729..12196
                     /locus_tag="PLS35_10595"
     CDS             11729..12196
                     /locus_tag="PLS35_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19157.1"
                     /translation="MLIKELLRLQATQTYLRAIVGGDFNFEHHSPEYQELLVAGLRDT
                     HMIASPRTDLYSSDPNQNTIVHQEELTMPPSLRQAMTNLPEAEQQRMAEGYQDGMGQA
                     RRIDYLFHMGKGPHLPNECLRQTLLGESAALSTPSGSDHFGVLNTYILDPSQR"
     gene            12353..12805
                     /locus_tag="PLS35_10600"
     CDS             12353..12805
                     /locus_tag="PLS35_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNI19158.1"
                     /translation="MSGKTKPTDPSAKMYYNDRWSKGARANTNGVKQAGIGCLLFVGG
                     AGSLEVKPGVQSVDTPEFLKDWKQGALATREVLNLLRKELSLDWSFLSPSADLSPGQR
                     TGNFRLGKDQLLVDAKGQSRISVEDYALAMIDEAEAPHHIRQRFTVGY"
     gene            12886..13635
                     /locus_tag="PLS35_10605"
     CDS             12886..13635
                     /locus_tag="PLS35_10605"
                     /inference="COORDINATES: protein motif:HMM:NF009385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI19159.1"
                     /translation="MNNHTLKGKVAGAGAKNLGGLLSREFAKGGAKVVVHYNSDTTKP
                     NADDTVKAIKDIGGEAYAVQGDLTKPTNIAALFKSAKDRFGGIDIAVNNVGKVLGKPI
                     VDTTEAEFDSMFDINAKAAYFFIKEAGTNLNNNGKIITIVTSLLAAFTDGYSTYAGGK
                     APVEHFTRAAAKEFGPRGISVTAVGPGPMDTPFFYGQEMSERVAFHKSQALNGNLTKI
                     EDIVPIVRFLATEGWWVTDQTIFANGGYTTR"
     gene            13931..14293
                     /locus_tag="PLS35_10610"
     CDS             13931..14293
                     /locus_tag="PLS35_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19160.1"
                     /translation="MSLLLDRILAADFEVPDVELLRSPTVVQTNNVAQYLHGTNRKDP
                     DDLTQDFPNIAPPWPNFFVEFAMPSTICEVTPEGSQMMQWEGPKTFGLHWMAGRRGSY
                     DFGLQQSLFKTLSESTNP"
BASE COUNT         3211 a         4007 c         4258 g         3293 t
ORIGIN      
        1 cgaagcgatc ggtttcgtgg gcgatgtcta tggggcagaa ctcggcaaag aagacctgct
       61 gcgctacggg cgctatatct tggtccgtgg atctgttgct gatctgagcc cccatctcac
      121 ggaggaagaa aaaaaagcgc agcatggcgg cagaattaca gcggggcttc gggtccggct
      181 ggcttcacag ggcgtgaccg gagtggtgta cctggaggcg gattacctgg acgcgcagca
      241 gtatccgaca ttagacgtgc cgtggaaacc tcaagctgtg tacctgcctt ccgctccgag
      301 cacggtgagc gtattgggcg ccgctctcca taccatagcc agagatttgg aaaaagccga
      361 catccacaca ataaccagaa atctcgatag tcttgtgctt gaagtgacga aattggtcaa
      421 agacacaaat gagcaggcat tgagtagccg gacgggacga gtgctggcgg agtttcaaga
      481 gacggcacga caaacgcgcc ggcttgtgga gagccctgaa atcagagcca tggtttccga
      541 tgcggccggc acggtgcaag gagcgagaca gctcatcgcc gatttgtcag aggcatcgaa
      601 acaaatcaag atcgcatccg aacaattccc cggcgttttc gcccggttgg agcgtacggt
      661 gcagcgaata gacaggctcg tgtcgaataa gagtcaagat atcgaggaca cagtggagaa
      721 tctgcgagtc atgtcagaga atcttcggga aataaccgac aatgcgaaac gatatcccgc
      781 tcaagtgttg ttgggcgaac ctccgcctcg ggcgggggcg acaaagcgat aacccgtccg
      841 ggatagcact gccgcatttc atcggcatct gccttgtcgt gttcgtactt ggcggttgcg
      901 tcaatctcag caagagttat ccggagaagc actcctatat tctggaggcg gtacattcag
      961 gcgagatgcg ggcctccatt cctggaaccg tcctgaaggt tcgaaaattt cgagtctcgc
     1021 caacctctga aggaaaggag ttggtgtatc gcacgagcga tgcacgttac gaaggagact
     1081 tttacaacga gtggtttgtc ccgcccaatg caatgcttac ccaacagatc atgaactggc
     1141 tgacatcggc cggcctgttt cagtatgtca tggattcgtc ggggccattg cctgccacgc
     1201 atatgcttga ggggaccctg acggcactgc atggagatta tcgtgcaacc cctgccaggg
     1261 ctgttctggc tgtccaattc ctcttcctcc atgaggcgtc cgctcaagga gaagttctgt
     1321 ggcatcgaga gtatcggaaa gaagtggaca tcatggaaca gaagcccgag gcactcgtgt
     1381 cgggttggaa tgaggctctt cgacttatct tgagcgctct tgatgaagac ctcactcgaa
     1441 cactccggcg tcagtgacgc gtgggcagat aaagccgtta cggccgtaat tgggacgatc
     1501 ttaccttcac gatccaggaa ggtcagccaa cggactggcg gcaagtgatc gatacgagtc
     1561 tcccgagccc ggaggacttt cgcagcccgg gagaagaaca aaccgttcca tcccttcact
     1621 acagagtgac ggcatggtca atcgaggtct tgttgcggaa ggcagtgtga gggagtagta
     1681 ggaggcacga ccttgcggat cggcatggca gcaggccacg acggattgaa gttgaagcga
     1741 aacgtagaag gacctttcag agaaggcctc ctatgcgctc gaggtcgtgc gccaaagcga
     1801 gagaatcctt ttcatagata ccccggttat gccggaatag taggtcttac ttcctaactc
     1861 tcttgggtaa agctatgtcc ctcgtgagct tgctgcgcgc gacttttcag ggcctccggg
     1921 atgacgaacg gacacaggga actcttgagg tcggtgagca gctgagctcg aagagaaatg
     1981 tgcggtggct ttggactggg gagctaattt cccaactcgg ggacggcctc aataggccgg
     2041 cgatcctgtg gttcgtgtac gatctcactg gttctgctgt taaaatgacg ttcattggtg
     2101 tccttcagac catcccgccc ttggttctcg gcccagttat cggtgtgtat ctcgatcgtc
     2161 tgtccgccga ctggaaactt cgcgtgatga tctcactgga tctcgcgcgt ggtgcacttt
     2221 tggcgtggct cgcgaccctc tctattctcg gtatgctgac cttacccaca ttgtacacat
     2281 tcgtttttct gatcgccctt gctgcaatgc cgtatgggcc ggccttgaac tcgaccattc
     2341 cctctctggt acaagcttcg cgcctgaccg ctgcaaatgc ggcaatacaa agcacggcaa
     2401 cgctcggctt gttgtgcgga cccgcgatca gcggcgccac agttgccttc cttggtgtgg
     2461 ggtacgtcct gtacgccaat gccgccacct ttctgctcgc cgctttctct aagatgccga
     2521 tacgcgtaaa ctcagaagca ccgagcccga gccggagtgg aacatccagt ggatggatca
     2581 atgaactggc agctggattc cggcttgtgt ttgttgagca acgaatcatt tttcgagttg
     2641 ccctgttagg cgccctcttc actctcgcat cgacaggctt catctttctc cttcccatca
     2701 tcaccaagaa cattctttct ggagggccga tgttccttgg ggtgatatgg tcggcccttg
     2761 gcctcggcat gatattcatg tcagtgctgt taatgtcgag cgctcggcga acactcatcg
     2821 atcatttgtt gctgatatcg gcggggccgg tgattgaagg gttggccatt gtggggctcg
     2881 cagcggttga cagcttggtg gcggcgcttg gtctcgtgat cgtcatcggt gccggaacgg
     2941 ctcttgtcat gccgatgatc acggccttta ttcaggcaac agctcctcag gagactcgtg
     3001 caagagcgtt gacgatcttc ggcactatca cgatggtctc cgccatggcg ggcatgacgg
     3061 ccttcagctg ggcagctgat catctgggaa gtcgtatcgc actcgttgtg cttggagtcg
     3121 tgcaaatgat ggccggtgtg atcggcatca tgtttacgca atcctcggag ttccagacaa
     3181 atctcgcgag tcggtccaat gatcatccgt caagcaaatc ggacaccggt atcacttgac
     3241 cccgtttcgt cgctgctcca gcgggttctg aataggacac aactttccag taatcggaga
     3301 cagagacgaa cattgaagtg ggaacagcag gttgtcggtg tctgcatcca gaggaagagt
     3361 agaggggagt gagacgaccg tgaggcgatc ggcatcatcc gggcggcgaa atctggcgca
     3421 agaggcgaca ggccctatca ggcgctcgcg gggcagtaag agcgattgtt tccgcaagta
     3481 ccgaaagacc cattgaacgg ctagtcacga cccatacccc ggtgccggcc ggacacgata
     3541 agtttccaga gacacttgcg cgccagatgc tcggcgatct cgcggtagct catcctcagg
     3601 cccgcaggaa tccttcgcaa tgcttgccac actcgcccct ggaacgccgt gctccgcaca
     3661 tccaacggca gatcgagacc gagcgacggc gcttcgacga agccgacgat tttcgcgacg
     3721 agctggtcga agtcggcatc acctccgatg agagtggctc gaggaaactg gtcttgtaac
     3781 tcgcgcgcca gcgcgtccgg atcgtcgccc agaaaaaggg cgcaaatccc gcgctcgctc
     3841 ttggccacca ggatcgaccc cagcgagcac tcgcccacgg cgaaccagat ctccgtccgg
     3901 gcgccaggcg tgaccaagtt tgcggtaggg cgggagcggc cgaacgaggg gcgtggcacg
     3961 cactcattcg atccgtttca tgaattcgat cgatcctttc cctcgagtca taccgcgagg
     4021 gcatttgccg tggcgagcgt atttgccgat cgctacgagc aaccgatccc gttgatcgcc
     4081 tatggcacgg cagcgttcat cagcgtctca cgggtgtatc tcaatgagca ctttgcctcg
     4141 gatgtcgtcg cgggtgccgt cttgggtttt gtgatcggga aggtcctgag ctggcgccat
     4201 aaggacgaga atcggcggtg gaccgtgctc ccttatgttc cggacgccag gggtgggttg
     4261 gggatgacgt tcagctgctc attctgactg gaagccggct caacggcctg gcgatgccga
     4321 tcgacatgcg atcgtaaaga ttgcggaacc ggctatggga gcttcttaga caaatggcaa
     4381 gaatactttc cgcgcttctc ctcagcgtcc tgtgtgcgat caatggctgt acttccgcac
     4441 aggtcacgca gacgcacgct tcgcagccga acgcatttca ggcagaaggc aagaaacagg
     4501 agcagtctca agaagcgcgg tctccagaag agcggcttcg aaaggagaag ccgcaagagg
     4561 aacgggatga ggaagacacg gtgctctccg caatcgcggc cgctccggag tacctcgtga
     4621 aaggattggc ttatccattc aagaagttcg ggatctatta cgagcgcacc gacctgatga
     4681 accgcctcat ggatttgttc tacaacgatg agcgtaccgg tggagtgttc ccgcgcatgg
     4741 ccgtgggcgg ggcgttgagc agcggcatcg gcttcaccgc gttccatcaa gacctcttcc
     4801 atcagaagaa ggaggtgcgg gctcgttacc tgtacgccac cagaggcaat caggctgcag
     4861 acttcgccta ccgggatccc agcttgttcg gctcgaaatt catgctcgat ctcgatgtct
     4921 tttggctgaa cttcgacaac ggattttttt tcccgggagg caatcgggcc gcggagaacg
     4981 gcaaaacgaa gtatgacctg aaccagttgt cgcagaatgc cagactcagc tatatggtgg
     5041 cgcccaacct caaagcgtcc cttctgggca ggatattgtt cacggacgca gggccttcaa
     5101 gccgaactcc cacgacccca ccgaccgtgc cagggatcga tacctatatg cagggagcgg
     5161 cggtagagcc aagccttacc tatgattcaa gggataaccc atttcgtccg agcaccggtt
     5221 tgtacatcga cacagggttc acctacacca accaattgaa cggggatcaa ttccgctaca
     5281 tggggtattg ggtcgacgtc cagcgataca tcccggtctt tcgtggagac cgggtattgg
     5341 tgattcgggc gtatctctcc aaactggatt ccctggacgg caagtcgatc ccgttctatg
     5401 aattgaatct cctcgatctg aataacggac tgcggggatt tgatcgggga cgctggcagg
     5461 acaagggagc gctcctctac aatctcgaat ggcgctatcc cgtgtggaaa gacgttgagg
     5521 gcacgatttt tctcgatcta gggcaggtat tcggcgacta caaggatctg caacccaagt
     5581 tgtttcaata ttccgccgga ggaggattcc gatttgtcac gtccaggagt tttgcctttc
     5641 gagtccaatt ggcggcaagc aatgacggct tgctggcctt gttccgggga gacttggagt
     5701 ttgttcggca tcgcggcact gtgctcggtg ggttctgaac tgcgatgcaa tcagagctgc
     5761 aaggcgagat agatccggcg atccgaggag gcgagagatg agggactgga actggaggcg
     5821 gcggatcagt gctttctctt gtgatggcgt cgccgcatgg tttctttgcg cgctcgccct
     5881 gtctgtgatc ggctgctata accgggttgt tttgcaagaa ccggtcgtat gggaagtcaa
     5941 tgatcggcgc cccatctccg aaccgcaaga ggaaaccatc ggtcagtggg cactctggga
     6001 tggtaccgac aagatggtgt tctatcggat cggtcaactc ttcaacctcg ggcgatcgat
     6061 gcgggatctg ggcgcttggg tcgggctggc cgatccgatc gaggcccaga acgtcaatgc
     6121 cttcgatgag gtgccgaact ccacctggtt tacgaatcgc cagtttttct cgaggcgctc
     6181 cgaagaggaa ctgacggctg gcccgacgac ggatcgcggc gctcccgata ccgcgggacc
     6241 gtggacggcg attgccgcga aggcaagcct cggttcgacg ccggggttcg tcatccggga
     6301 tcagaaaaac gatgtctatc tgatcaaatt tgatcctccc ctctatccgg agatgaccac
     6361 ggcttctgaa atgatcactc cgcggtttct gcacgcggcc gggtataacg tacccgagca
     6421 ttatctggtc tacttcgatc ccgccaagat caggatagac ccgaaggcca agttccgtgg
     6481 gaagtatcat gtcccgaggc cgatgcagga acaggatatc acggccattt tggaccggat
     6541 tcctcaccgg ccggacggga ccattcgcgc catggcaagc aagtacctgg cggacgttcc
     6601 gaaaggtccg ttcctctacg aaggaaagcg tcccgatgat cccaatgatc ggatccttca
     6661 tgagaatcga cgtgagttgc ggggctttcg catcctcgcg gccttcgtga atcataccga
     6721 taccaaagcg gcgaatgcac tcgatacata tgatcccgag acacgctatc tcacgcatca
     6781 cctcatcgac ttttcctcga ccctaggagc cgacaatgcg gatccgcagt ttccccggtt
     6841 cggcaacgag tatttctttg attttggtac cgtcgggcat tcaacaatcg ctgtcggcag
     6901 ctacgtcagg ccgtgggaaa ttcccctgga gatgcactat ccggccgtag ggtactttga
     6961 atccttgtat ttcgatccgc cgcggtggcg gcccacctat ccgaacccgg cctttctccg
     7021 catgacattg cgcgatgctt actggggcgc caagatcgtg acgtccttta ccgacgagga
     7081 tattgacgcg attgtccata ccggaaggta tacggatgcc agggcagaac gctacgtcgc
     7141 ggacgtgtta aaagcaaggc gcgataagat cggacgctac tattttaact tgatcaaccc
     7201 cctcgaccgc tttgctatcg acaccaagtt cgatgagcag gtactggtct tcgagaatct
     7261 ggccctctcc ctcggatatg cgccggctcc ccacgcgctt taccgttatt ccatcagcaa
     7321 atacgtgccg aactggtttg acacatccgt gatccaggaa accctggttc agtcgcccaa
     7381 gatccgtctg gacaaagcgt tcctagaagc ctggggaaaa cacaccgccg atggccgcgc
     7441 aacggaggag gagcggccga tcgcggcggt gagaattcaa accagctatg atgatggatc
     7501 tcactggggc aagcgtgtcg tcgtgtacct tcgagaacat ccggggacca ggcgcatgac
     7561 gattgtcggt ctggagcggg aaacctagat tgttcgcatg gcgaactcga tgaacttcgt
     7621 cccaatcctc caaaaactag tcgatatcag gagcggtgaa tatcggaaaa ccgttctgat
     7681 gttcctctat atctttctcc tgttactctc gtttgtgatt atcaaggcgg tgcgcgatgc
     7741 actctttctg gtcaggctgg gccctgagca gctcccctat atttacattc tggtggccgt
     7801 gatcgtcgga gtggtagcct ggatccatgc gaagctttcg gcgcgccttc gcctcgatgt
     7861 gctgattcgc gggacgctgc tcattgtcgt tgccaacctg ctcatcttct ggtggctttt
     7921 tcggttcggc tggccctcgc tctactatgt gttctacatc tgggcagccg ccatcggtct
     7981 tctcacgatg tcccaatatt ggctccttgc caattccgtc ttcaatgcga gagaggccaa
     8041 gcgactcttc ggcctcatcg gtgccggatc catcctcgga ggcctcgtcg ggggagtcgt
     8101 gaccgcgaga gccgtgactt ggatcgggac cgagggcctg ttgattatcg ccgcgacggg
     8161 gtacctcttg tgcggcggtt tggccttgat cgtgggtcgt gctgctgtgc cggcggctcg
     8221 tccttccggt aacgcatccg gaatgtccgc tcaagcggaa gcggccgctc attccgaaga
     8281 atcgatcctg acgacgatga ggaattctcc gtatctcatc acgattgccg gtttgatcgg
     8341 gataggagag atcgtcgtga tgctcgtcga ttatcaattt aaggtgatcg ccacgcagtt
     8401 atttccgtcc aagaacgacc taacgggctt catgggaatc ttttcggcat tggtcagcgc
     8461 ctcgtcgttc ctcgtgcaag tgttcttcac cggaagaata ctgaggagcc tggggcttgg
     8521 tttggccctg ctgtgcctcc ctgtcggctt actcttcggt tctgtcgcca ttgccgtggt
     8581 cccggggctc atctcggcag tcatggcgaa gctcaccgag ggcagtttgc gattctcgat
     8641 caacaaagcg agtctggagc tgttgtacct gccgctgcca tgggcaataa aggccaaaac
     8701 caagccattt atcgacccag cttccgagcg tgtcgcagga ggcatcgggg gcctcattct
     8761 gatcgtcgcc accagcatcc tctcgctgtc aactgcacag atcagcttcg tgaccatcgg
     8821 actgctcagt ctctgggtcc cattggccct ggccggccag agacaatacg tcgattcttt
     8881 gcgcctcgcc ttggagcgga ggcgactcga tctgacattc ccgattctga atctctcaga
     8941 tcgacggacc gtcgatctgt tgatacgcca cctccttgat tcggacgatg ccgggacgtg
     9001 ctacgttctc aggctcctgg agcaggcgga tgtcaagccg tacatcccgg ccttcaaaca
     9061 attgctcacc agtcggtctc ctgaggtccg ggccgcggtg attcgcctcc tcagcgaaat
     9121 cagggaaccc tctctcgtgc cggcgctgac agacgtgctt ggggatcagg aaatggagat
     9181 tcttgtcggc gccgtgcgct ttcttgatgc acatcccggc tcttcgggcg cgaagctcga
     9241 agaatggatg gcgagcaccg atccgcggct cgcggccgcg gcgatcgtcg gtctcctgaa
     9301 cgacccggcg caaaggttgg gtgcgagggc tgaacaggcg ctccgctcga tgattcaagc
     9361 ccacggccct caaggcgaga ttcgtcgccg ggaagccgcc aaagctttgg gttggattca
     9421 tcccgaggcg actgcttcgg atgccctgat acggctgtta caggatgact caattgaggt
     9481 cgtgatcacc gcaatcaaga gcgccggcag gatacagcgc agagaatttg tttctccgct
     9541 tgtcgctttg ctggtgagaa aggagacgaa aggcgaggcc aagaccgccc tagtcgggta
     9601 tggctccaaa attctggggg gtcttgccga tttgctcgaa gatgacaacg aacgaatgga
     9661 gctgcgacga acgcttccct cgctgcttgc cgagatagga ggggaaaggg caatcgccat
     9721 tctcctgcac aatctcaatc agcccgaccg attgctccgt tatcaagtcg tgaaggcgct
     9781 caacagaatt cgcgcgcatg atccgaattt ggagccggag ccggaactca tcgacgccag
     9841 cctgaccagc gaactacggc accattatga gctgctgctc ctctttcaca gtccttcgtt
     9901 ggtcgaggat agccaggggg ggcgactcct caaaaaggcg ctacaggaac ggttggacca
     9961 tcagctagaa atggtgttcc gtcttctggc gcttcgattt cctgtgtcgg acatccggga
    10021 cgcctatgcg ggtctcactg tggatgacgc ccgccttcgg gcaatagcca cggagttcat
    10081 ggacaacctt cttccatccc atctgaagcg atatctgatt ccgatcatcg acaactggcc
    10141 gtcggacgcc gtggtggaag tcggcaggca attcgtctcg atcaatgggt ccacacggga
    10201 agaacttatc ttgtacttgc ttaagggaac ggatctctgg ttaaagacat gtgccattta
    10261 cttagcaggg gaatggaagc tctcatctct gctcagcgaa ctgcggcaag ccgctgatga
    10321 ctcaggacac ttgatcaggg agacggttca cctggcgctc gaacaactta acgactagac
    10381 cgaatgctca ccacgattga aaaagtcctg ctgttacaag gtgtcgatat tttagtggat
    10441 acggaaactg agaacctggc caagatcgcg gccattgccc gggaagtccg tttcgaagag
    10501 ggggcgacga tttttgagga gggcgagcca agcaatgcgt tctacattgt gctggaggga
    10561 acggtctcgg taaggaaagg ttcgaaggag ataggactcg ttaaggacaa ggagacggtc
    10621 ggatgggtca acttgcttga gcagaccccg agaaccgtga ctctggtggc gttaaccggc
    10681 accacgttcc tcaagatcga gcgagaaggc ttgtttgacc tgctcgctga tcatttcgac
    10741 attgttcgcg gcatcctcaa gcggctgtcg cggattatca atcaactttt ggacagatga
    10801 gatgagtccg atttatcttc tgtcgtgtgc cttaatcgtc atcgcgagct cgatgcttgg
    10861 ttgtgaggta agagtaagaa accttccaca tacagtggaa taccgttcgg agcttgcatg
    10921 tgaaccaagc tcgcaagagt gcccctgggc taatctggaa cgacaagcca aattcaccgc
    10981 gcttgttaat tgcctcctgt cgggtgcgac gttctttcgc attgacgact ctcagacgac
    11041 caatggctcg gagccttctc tggtaactcc cattgagcca agatatgggt atgaacgtgc
    11101 gacggatcat ggagccgggc aggcctgtcc atcccagaaa acggtctgcc aagtggccag
    11161 ccggcatgag agggaactca cctgggccac ctgtggcaca cagcctttga aagatgcgcc
    11221 cctgcgcggc ggaaatacct tgacccatgc ggatagattg accgaaagca ttgtggtgac
    11281 cggacgttta agtgagggaa ccgacgagtt ccactctccc catgggttag acggtcccga
    11341 tcacttctac accttcgcct tggctcagcg gactcgcatg gagatagcgg tggcagccaa
    11401 tacttcgcac tggtccgatg cggtggggct cacccgggca gcctggcaac cggcattgta
    11461 tctcctctct gccgatgggg cgatgctcaa ggagggacac gtcttccgtg ccggtgtcac
    11521 ctctttgttc cccgcggaat ttgagcctgg gacgtattat cttgtggtgg acagttccat
    11581 aagagaatgg tcgcgggggg acgggtacta tcgcctctac gtcgggttca acgaggaagt
    11641 tatgggccca ctagtcccat gatttgctgg cgtgcgagac tttgttgccg cgttggagag
    11701 ccaaaacaga cggatcaagg agactcgagt gctgataaag gaactattga ggcttcaagc
    11761 aacgcaaacc tatttgagag cgatcgtcgg aggggacttc aactttgaac accactcccc
    11821 ggagtatcag gagttgctgg ttgcaggcct gagagacacg cacatgatcg caagcccccg
    11881 cactgacctg tacagctccg acccgaatca gaacacgatc gtccaccagg aagaactcac
    11941 catgccaccc tcccttcgcc aagccatgac gaatcttcca gaagcggaac agcagaggat
    12001 ggccgaaggt tatcaggacg gtatgggaca agcgagacgg atcgattatc tgtttcacat
    12061 gggcaagggc ccacacttac ctaacgagtg tctgcggcag acgctgcttg gagaatctgc
    12121 ggctctctct actccgtccg ggtccgatca cttcggcgtg ttgaatacgt acattcttga
    12181 cccgtcgcag cgctgatcat ttctagctgg agggctgggg acagcgattc tctggccgag
    12241 gatcgcagag ccggaggctg ctgtggattc ctgtgtgaat ggcaggcgag cggccatcga
    12301 tgaaggtcta caagacgaag gacaatgaca gtggacaccg ctgagtcgta tcgtgagcgg
    12361 caaaacgaaa ccgactgatc cctccgccaa gatgtactac aacgaccgat ggtcaaaggg
    12421 tgcacgggcc aataccaatg gtgtgaagca ggccggtatt ggatgcttgc tcttcgttgg
    12481 cggcgccggt agcttagaag tgaaacctgg ggttcaatcc gtcgatacgc ctgagtttct
    12541 gaaggactgg aagcagggcg ccctcgccac ccgggaagtc ttgaatctgc tgcgcaagga
    12601 actcagcttg gattggtcct ttctctcacc ttccgctgat ttgtcccctg gccaacgaac
    12661 aggaaacttc cggcttggga aagatcagtt gctggtggat gcgaaagggc agagccgcat
    12721 ctcggtcgag gattatgcct tagctatgat tgacgaggcc gaggcgcctc atcatattcg
    12781 ccaacggttt acggtgggtt attgaattga gatgtggaaa ttcctctgcg cgcgagcacg
    12841 ttctgattcg cgccacaacc gacttgatag ggaaggagag acattatgaa caaccatacg
    12901 ctgaaaggga aagtagctgg cgccggcgcc aagaatctgg gcggtctcct cagccgcgag
    12961 ttcgccaaag gtggagccaa ggtcgtggtg cactacaaca gcgataccac gaagccgaac
    13021 gccgacgaca cggtgaaagc catcaaagac atcggcggcg aggcctacgc cgtccagggc
    13081 gacttgacca agcccaccaa tatcgccgca ttgttcaaat ccgccaagga ccgcttcggc
    13141 ggcatcgata tcgctgtcaa caacgtcggc aaggtgctgg gcaagcccat cgtcgacacc
    13201 accgaagcag agttcgactc gatgttcgac atcaacgcca aagccgctta tttcttcatc
    13261 aaggaagccg gaacaaatct caacaacaac gggaagatca tcaccatcgt gacctcactg
    13321 ctcgcggcct tcaccgacgg ctattcgacc tacgccggcg gcaaagcgcc ggtggaacac
    13381 tttacccgcg cagcggccaa ggagttcggt ccgcgcggta tttccgtgac cgcggtcggt
    13441 cccggcccga tggacacgcc gtttttctac ggccaggaaa tgtctgaacg agtggctttt
    13501 cacaagtcgc aggccttgaa cggcaatctc accaagattg aggacattgt gccgatcgta
    13561 agattccttg ctaccgaggg ttggtgggtt acagatcaaa cgatattcgc caatggtgga
    13621 tacaccactc gatgattcga ttggtgtcaa aaccctaaca gtcggcttca gcagcgataa
    13681 gcgcgtcaca ctgcgggcaa gagtgatgag ttgcgccaaa tggtgaagca gcatttgact
    13741 gtgtggcgga ggagaagcag gacggccagg tcgtactctt tgctcggaga acgcgcacgg
    13801 tgaagctgtg cacgatcgat gcgcgcaggg aagcttaagc gggccagcca agttctattc
    13861 agattgtttc aagaaggggc aaattgaagt ggtgaggggt ttaaagcggt tttccgaata
    13921 gggctattac atgagccttc ttctcgatcg catacttgca gccgatttcg aggttccaga
    13981 tgtcgagttg ctaaggtcac ctacagttgt tcagacaaac aatgttgccc agtatcttca
    14041 cggtaccaat cgtaaagatc cagacgatct tacccaggac tttcctaata ttgcacctcc
    14101 ttggccgaat ttcttcgtgg aattcgccat gccttcgact atttgtgagg taactccgga
    14161 aggatcgcaa atgatgcaat gggaaggccc aaaaacattt gggttgcatt ggatggcagg
    14221 gagacgaggt tcttacgact ttggtttgca gcaatcgcta ttcaagacac tttctgagtc
    14281 caccaatccc tagatacgtt caaaatatct agaagctgca gaacaagccg cttggttcgg
    14341 gcacgttcat ctcttcttgg ttgtttctta cagtcgagta aagggaaatc actttgccac
    14401 attgccctcc ccgtttactc tgggacgtta gacgggagaa atagttgcgg gcctaagtcc
    14461 gaggcggtag cgaactcggg caacaggaca atgattttca tcatctgaat aaagcgggca
    14521 caatgaaaat gtgggtcaag tgttgtctca tgatcatgct gtggttcacg gcttcgccat
    14581 gtattgccaa tgccgcagta gtcagcgtgg aaggactgcg tgtctcatct ggcatgcgat
    14641 gttggtacag cggcctccca tgacatccac gaccgttgag gtattgtgcg gtctgctaga
    14701 ctgccactcc ttcgcccgtc atgtctctag accgtcgcct tcacagacgt gataagatag
    14761 actatcacg
//
LOCUS       DAOQEW010000087        14519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1167_length_14519_cov_8.218404, whole genome shotgun sequence.
ACCESSION   DAOQEW010000087 DAOQEW010000000
VERSION     DAOQEW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14519
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1167_length_14519_cov_8.218404"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLS35_10615"
     CDS             <1..948
                     /locus_tag="PLS35_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19161.1"
                     /translation="ALLDDDDEFLTHKLMVQVPILEAHPEIGVLYGQSVVTGGETPLL
                     LWPEWGPSGNVFEEFVTTTDDFLHPPTWLVRRELFEQAGFFDESKAGMEHYDMALRIA
                     ALTPWMFLSGGPVARGRYSQQGLWYSTIVNGTNEQQLPKIIEAALLRLPATADADRVR
                     RKARAAVCASIAHQRWWAGGGLEPTKAHLLKAVREAPWLLQEAAVVEWLKRIASTLAG
                     SSSHPVAAVKKFWKEITNAVAEAETIYTGSLRRLFGELLEAAAISMKRGSPRRAPLVA
                     VSALLNDPSSWMQPARLYSLYRAWTRESLATAEHAKVSP"
     gene            984..1859
                     /locus_tag="PLS35_10620"
     CDS             984..1859
                     /locus_tag="PLS35_10620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI19162.1"
                     /translation="MTLSIIIPCRNAARTIEETLQALADQKWPAAWEVIVADNGSTDN
                     LARVVAKYTLRIPHLRLVDASARRGASYARNIGVNAATGEYILFCDADDVPAAGWAVA
                     MEKAVRTHGFVASRLEFEKLNSPVVRSARGCTQIVGLQQFRFLPFPHAGSGTLGITRA
                     LHDAVAGFDETIPICEDIDYCMRVQQQGIKLEFVPDAVLHCRLRASKKGVYAQASQYA
                     EYEVYLYKKYGAGHLWEWWRWRKYLQAWRNLLGRMSELLRTPEGKTLFAWRLGRLMGL
                     LKGSLRFWAPPVMVE"
     gene            1876..2607
                     /locus_tag="PLS35_10625"
     CDS             1876..2607
                     /locus_tag="PLS35_10625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNI19163.1"
                     /translation="MSLQSFLSTSVRKVFGSLTHVVTPSAVLALTFDDGPDPENTPKV
                     LALLEKYQARGTFFVVGQAAAVQPALVQRIAAGGHAIGIHSWDHRAFPGLTSRERRRQ
                     IRECERALRPYVSPLFRPPYGEQTVMSRLEAFMMGYEVVGWNLNSGDWYESQSSVMGK
                     YLLEMVSPGGIVLFHDTLYDKGTPAHGTDSEHTSWPDRQAMLDALETMLDRLSGSYRF
                     VTVPELLQAGRPRRSFWFKPSLATA"
     gene            2648..3463
                     /locus_tag="PLS35_10630"
     CDS             2648..3463
                     /locus_tag="PLS35_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HNI19164.1"
                     /translation="MSSVAVVVPGYNRAEFTEDEEISFRHLEHYLGRYDKFLVVPQSL
                     AIERPGFHIQRFPDSYFGSAIANARLMLSPTFYGAFQSYRYVLIYQLDALVFSDRLME
                     WCASDWDYVGAPWLKCADSPWVGASRVGNGGFSLRKVSSFLRVLSSDAYWVDPEVYWQ
                     RITTGQSWYVKSVNLPRKWYKQIKRFNNVKRELERWHLRPDGTKNEDHFWADEAVRYD
                     AQFKVAPFHVGLDFAFEVVPRHCFELNQNRLPFGCHAWPRYDRSFWEPYLIKP"
     gene            3480..4364
                     /locus_tag="PLS35_10635"
     CDS             3480..4364
                     /locus_tag="PLS35_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI19165.1"
                     /translation="MNESELQQASPLTAGTLAPAVRTPTVKIRPSRGLFHLDLQSVWQ
                     YRELLLFLIWRDTKVRYKQAVIGAGWAVFQPLISMLLFTVLFSYLAKLPSDGVPYPLF
                     AYAGLLPWNFIAQATSRSGTSLVGESHLISKVYFPRLIIPLAAASAPAVDLLCALLMM
                     IPLMLWFGVTPGWQLLFFPVFVGIALLAALAVSFWFSALHVKFRDVGHIIPFFVQFWM
                     FASPVVYPASLIPEKWRTLYSLNPAVGVVEGFRWSLLGQRPPSLETILPSVGIVLVLF
                     VSGIVYFKRMERTFADVI"
     gene            4364..5623
                     /locus_tag="PLS35_10640"
     CDS             4364..5623
                     /locus_tag="PLS35_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19166.1"
                     /translation="MAEIAISARHVSKEYVIAEINHDTLRDRIAHGCRSLLGRRAAKS
                     VPPKSFQALNDVSFNVMEGEVLGVIGHNGAGKSTLLKILSRITEPTGGEVDLYGRVTS
                     LLEVGTGFHAELTGRENVYLNAAMLGMRKVDINKRFEAIVEFSGTQQFIDTPVKRYSS
                     GMYVRLAFSVAAHLDPEILIVDEVLSVGDGAFQKKCLGKMDEVRRDGRTVILVSHDLP
                     VVASLCQRAILLRHGRVVGDGQPQQIIEQYMSEAFTGTGVSLADRTDRYTKGELTVQA
                     ITFLDDKLNPLQPALSGQHLVIRVQYKCHVRKAFKDMRVMVVINRDERTSCIFSTDLV
                     SHTPLDLEGSGYVDFHVPHLPLCGGRYFLHAAIENNTMTQDWVQYAAELQVLDGDYYG
                     TGRMYPHDGWRGKGMFVDHSWRFERTS"
     assembly_gap    6079..6178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6179..9772
                     /locus_tag="PLS35_10645"
     CDS             <6179..9772
                     /locus_tag="PLS35_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI19167.1"
                     /translation="RFELAAECADVGVWEWDLVTKSAYYSPRWKTQLGCTESDIGPTV
                     ADWESRLHPDERAQVLATVTLFQQGYTKQYRLDHRLRHQDGSYRWMAALGAGVYDEQG
                     CMIRMTGIHLDITVRKQAEEARRESEERYRELVEQAGDIIYRTDAAGRFTYCNPTSLR
                     ILGYFPEELLGRHYLDIVEPQSRAQAERFYGRQFIRKTLRTVYELPVVTKDGREVWLG
                     QHTQLLMEGNTVHGFQVVAREVTERKRVEQALRESEERFSKAFHSSPAGMAISRLKDG
                     RVLDVNEAFVRVSGFSREELIGMSSLDIGIWINPDDRRHLAEALRRDGFLRHLEKEFR
                     TKSGELRHGLFNVEPVCISHENCVLTLVLDITRRTEAEAALRRSQTVLQAHQHALMRL
                     TKSQHIGSGDWPSALKELMQTSAEAMAVDRASVWLLDRTGAVLECVELYQRNEARHSR
                     GARLDVAQYPRYFAELLEEQVVDAHDVASDPRTAELLQPYLQTMGIASLLDVPIFFGG
                     RLAGVVCHERLGSARPWSNEDAQFATSIGNLVTLAYESKQRREAESALVTAKEAAEFA
                     SRAKSDFLATMSHEIRTPMNAIIGMADLLSETPLSEDQREYVQIFRDAGGNLLSLIND
                     VLDLSKIEAGHLDLDLTDFDLSDLVQRAAELVAVRASEKNLELAYQIQPDVPTSLVGD
                     PNRLRQVLLNLLGNAIKFTDQGEVVLRVECDPHAEGPGSLLFTVRDTGIGIPRDKLAV
                     IFERFTQVDSSMTRPYSGTGLGLTISRRLVERMGGRMWVESEPGRGSTFSFTAKFAAH
                     AQPASPVPTAQWEQLAGLRTLIIDDNATNRLIVRETLSSWGIPSAEAAGGDEALLELR
                     RALTAGMPYRLAILDVRMPKLSGWQVAETIARSPGLAGLSIIMLTSERRAGDQARARE
                     HGVVRYLTKPFRRSDLFNAMMAVLGKTAPAGNPESEAPSQPKFAGDDQSGLKILLAED
                     FLDNRRMMEFYFKATPHRVATAANGQIALEMFMHGSYDLVLMDIQMPVMDGYAATRAI
                     RAWEKDQRRRSTPILALTANALQSEVQRSLDAGCTAHLTKPIRKAQLLEAIQLYFQTA
                     SSQPPVPAYHEEPAVLHVNVELEALMPGFLEHRRQDLIQMTAALDREDFEVMREIGHG
                     LKGAGGTYGLEAITAYGRSLETAASRKDGPALREILERLRRFLDRLQLIYV"
     gene            9785..11179
                     /locus_tag="PLS35_10650"
     CDS             9785..11179
                     /locus_tag="PLS35_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI19168.1"
                     /translation="MRVLLVEDHPDVRLLFEQVIGARGHEVTACADGESAWEAYGKRS
                     YELVLLDWELRGGGMDGLQLCRRIRSGPGGDRCVIVMITGHDSPESLRAALQAGVNDY
                     LAKPVGVEFLRLRLTIAEQWVESVRRRFAAEDQAQALQSQLADHGKFHDLIGRSPSMV
                     VLYEEIQQIAAVDATVLIEGETGTGKELVARAIHLSSRRAPHTFLAVNCAGFTDSILG
                     SQLFGHKKGAFTGAIDNQEGVFEAAQGGTIFLDEIGDISPAVQTSLLRVLQEREVTRL
                     GESKPRKVDVRVVVATHHNLVEDVEKGTFRRDLLYRIKVARLQLSPLRERPTDIPLLV
                     HAFLGQFRSVMEKPVLHVSPEALQVLTSYSWPGNVRELKSAVESALIHCKGTVVRPED
                     LPPEIRSPQPQATYVPLQRQDERTRMLGALQQTGGNRSEAARLLGMSRRTFYRRLAEY
                     DVAASAEPGAESFL"
     gene            11500..11784
                     /locus_tag="PLS35_10655"
     CDS             11500..11784
                     /locus_tag="PLS35_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19169.1"
                     /translation="MVEQQGPSQKEGSMEVEGFLLSNDMDQEVLVTIPPSSVGPFDQP
                     AVKERIDGYWIEMSRLPNRRWSDRTRAVGAVLTYLQGLSAISSGFATHTR"
     gene            11821..12138
                     /locus_tag="PLS35_10660"
     CDS             11821..12138
                     /locus_tag="PLS35_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI19170.1"
                     /translation="MWNDQNLLRSAREDNARDGAAGLSVVPGVSLTAKRRPEDLTPRE
                     QEILQLVWAGLTNRTIAEQLHISIKTAEAHRANMMKKLRVSNIAQLLKTALEEGLLAT
                     HAG"
     gene            12287..12679
                     /locus_tag="PLS35_10665"
     CDS             12287..12679
                     /locus_tag="PLS35_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNI19171.1"
                     /translation="MVEQRIEEITRANLAFYAAFESLDMLQMDKVWAHLEYVTCIHPG
                     WTLRSDWPAVRDSWVLIFNNTFSMKFELSDVQVQVAGDLGWVICTEHLTSRQDDQPVE
                     TRVLATNLFERIGDEWLMIHHHGSPVMG"
     gene            12861..13190
                     /locus_tag="PLS35_10670"
     CDS             12861..13190
                     /locus_tag="PLS35_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19172.1"
                     /translation="MAQITTLSDESCRQTVRGQLESILVEEGERPEMATRLAVNTTVV
                     LATGSLGPRPFGVSSPSGTDYSFFVQRKGESCLLRLYGRQKGFTRYTNNLTYISTRPL
                     EGCACAE"
     gene            13584..13829
                     /locus_tag="PLS35_10675"
     CDS             13584..13829
                     /locus_tag="PLS35_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19173.1"
                     /translation="MMVKGFLLKDREEQEYLLAIQPATHGMFERLIVRQKIDGCWVEL
                     NGNAQGTWQPRTQAITAVLGYLQRLWDTSKPVAAREN"
     gene            13886..14224
                     /locus_tag="PLS35_10680"
     CDS             13886..14224
                     /locus_tag="PLS35_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI19174.1"
                     /translation="MQAEKQDRGGDTMSGSSMKSGEMSADPDAASLKRKPEDLTPREL
                     EILRLIWDGHTNRSIAELLNISMKTADTHRANMMKKLRASNTAQLLKAAIEGKLLHIH
                     EGARRSDGAK"
     gene            complement(14308..>14519)
                     /locus_tag="PLS35_10685"
     CDS             complement(14308..>14519)
                     /locus_tag="PLS35_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19175.1"
                     /translation="GCQARVYAPAGTRSICKDHFLGFLTWRRRRGPQMFRKYAGMTME
                     ERDVIAGEWLQTLGTKDLPHPIPGL"
BASE COUNT         3014 a         3768 c         4360 g         3277 t
ORIGIN      
        1 gctcttttgg acgatgatga cgaattcctc acccataaat tgatggtgca ggttccgatt
       61 ttggaggcgc atcctgaaat cggtgtgctg tatgggcaga gcgtggtgac agggggagaa
      121 acgccgcttt tgctgtggcc tgaatggggg ccgtcaggca atgtatttga ggagtttgtc
      181 acgacaaccg acgattttct ccatccaccg acttggttgg tgcgacgcga gttatttgaa
      241 caggcagggt tcttcgatga gagtaaggcc ggaatggaac attacgatat ggcgcttcgt
      301 atagccgcgc tgactccatg gatgtttctc tccggcggac ccgttgcgcg gggacgctat
      361 tctcagcaag ggctgtggta cagcaccatt gtgaatggaa caaatgaaca gcaattgccg
      421 aagattatag aagccgccct attgcgtctg cctgccactg cggatgctga tcgtgtccgc
      481 cggaaagcga gagcggctgt ttgtgccagt attgcccatc agcgctggtg ggccggtggc
      541 ggtctggagc cgaccaaagc tcatttgtta aaagccgtgc gcgaagctcc ttggttacta
      601 caagaagcag cggtggttga atggttgaag cgtatcgcca gcaccctggc agggtcttcc
      661 tcccatccag tcgcggctgt aaagaagttc tggaaagaaa tcacaaacgc agtcgcagag
      721 gcggagacaa tttacaccgg ttcattgcgt cgattattcg gagaacttct tgaggcggcg
      781 gcgatcagta tgaagcgtgg ttctccaaga cgcgcccctc ttgtagcagt cagcgccctg
      841 ctgaatgatc ctagctcctg gatgcagccc gcacgtcttt attcgctgta ccgcgcttgg
      901 acgcgagaat ccttagccac agcagaacac gcgaaagtct ctccgtagcc gtattcgccg
      961 aggactttga cgtccgagga accatgacac tcagtatcat cattccgtgc cgtaatgctg
     1021 ctcgcacaat cgaggagacg ctgcaggcgc ttgctgacca gaagtggccg gcagcgtggg
     1081 aagtcattgt cgcggataat ggttccaccg acaacctggc gagagtagtc gcgaagtaca
     1141 ccctgcggat accgcatctt cgtcttgttg acgcctctgc acggcggggc gcttcctacg
     1201 caagaaatat aggggttaac gcagcgaccg gggagtatat tttgttttgt gatgcggatg
     1261 atgttccggc ggcaggttgg gcggtcgcca tggaaaaagc cgtacgcacg cacggatttg
     1321 tggcctctcg tttggagttt gaaaagctca acagtccggt tgttcggtcg gcgcgtggct
     1381 gcacgcagat cgttgggctc caacagtttc gatttttgcc atttccgcac gcaggatcgg
     1441 gaacacttgg tatcacacgt gccctgcatg atgcagtggc cggatttgat gaaacaattc
     1501 cgatttgtga agatatcgac tactgtatgc gagtgcagca acaagggata aaactcgaat
     1561 ttgttccgga cgcggtattg cattgccggc ttcgggccag caaaaaaggc gtctatgccc
     1621 aggcctcgca gtatgcggaa tatgaagtct acttatataa gaagtatggg gctggtcatc
     1681 tgtgggaatg gtggcgttgg cgcaagtact tacaggcgtg gcgtaatctg ctcggacgta
     1741 tgtcggaact gcttcgtact ccggaaggga agaccctatt cgcctggcgt cttggccgtt
     1801 tgatgggcct gctaaaggga agcctccgat tctgggcccc gccagtgatg gtggaatgac
     1861 gcgggaatcg tttccatgag tcttcaatcc tttctgtcaa cctcagtgcg gaaggtgttc
     1921 gggagtctaa cccatgtcgt cacgcccagc gcagtgcttg ctctgacctt tgatgacggt
     1981 ccggatcccg aaaacacccc caaagtcttg gctcttcttg aaaagtatca ggcgcgtggg
     2041 acgttttttg tggtggggca agctgctgca gttcagccgg cccttgttca gcggatagct
     2101 gcgggagggc atgctatcgg tattcactcc tgggatcacc gcgcatttcc cggtttaacc
     2161 agccgggagc gtcgccggca aatccgtgaa tgcgagcgcg cattgcggcc ctatgtcagc
     2221 ccattgtttc gtccgcctta tggagagcag accgtcatgt cacgcctgga agcgttcatg
     2281 atggggtacg aggtcgtcgg atggaatctg aacagcggag attggtatga atcccagtcc
     2341 tcggtaatgg gcaaatatct gttggaaatg gtgtcgccgg gcgggatcgt cctcttccac
     2401 gatactctgt acgataaagg tacgcctgct catgggaccg attccgaaca tacatcgtgg
     2461 ccagaccgtc aagccatgct cgacgccttg gagacgatgc ttgatcgatt gagcggaagc
     2521 tatcggtttg tcacggtgcc ggagctgctt caagccggac ggccaagacg ctccttttgg
     2581 tttaagccat cgctggcaac ggcctgaatg cgaagacaat gacgggagct caagaaagag
     2641 gcagaggatg agcagtgtgg cggtggtggt gccgggatat aacagggcgg aatttaccga
     2701 agatgaagag atttccttcc ggcatttaga gcattacctc gggcgctatg acaaattcct
     2761 cgtggttcct caaagccttg ccattgaacg gcctgggttt cacattcaac gatttcccga
     2821 ttcctatttt ggaagtgcca tcgcgaatgc gagattgatg ctctccccaa cgttttatgg
     2881 cgcatttcaa tcgtaccgat atgtgttgat ctatcagctc gacgctttgg tattttccga
     2941 tcggttgatg gagtggtgcg caagtgattg ggactacgtc ggtgcccctt ggctgaagtg
     3001 tgccgatagt ccgtgggtcg gcgcgtctcg tgtcggcaac ggcggtttct ctctccgaaa
     3061 ggtgtccagt tttttgcgtg tcctgtcctc ggacgcctat tgggtggatc ctgaggtgta
     3121 ttggcaacgt attactaccg gacagtcctg gtacgtgaaa tctgtcaatt tgccgagaaa
     3181 atggtataag caaatcaagc gattcaataa cgtgaagcgg gaattggaac gttggcatct
     3241 gcgtcctgac gggaccaaaa acgaagatca tttttgggcc gacgaagcgg tgcgctatga
     3301 tgcccaattc aaggtggcgc cgtttcatgt cggattggat tttgcctttg aagtagtccc
     3361 acggcattgt ttcgagctga atcagaatcg attgcccttc ggttgtcatg catggccacg
     3421 ctatgatcgc tccttttggg agccatatct catcaagcct tgatggaatc gtcatactta
     3481 tgaatgagtc ggaattgcag caggcgtcac cgttgacggc tggcactctc gcgccggcgg
     3541 ttcgaacgcc cacggtcaaa attcgcccca gtcgaggtct gtttcatcta gacctgcagt
     3601 ctgtctggca gtatcgagaa ttgctgctct ttctaatctg gcgggacaca aaagtccgtt
     3661 acaaacaggc agtgatcggc gcgggatggg cggtgttcca gccactcatc tccatgctgc
     3721 tgttcactgt gctgttcagt tacctcgcga agttgccgtc tgatggcgtc ccctatccat
     3781 tattcgccta tgccggccta cttccgtgga acttcatcgc tcaggcgacg agccgcagcg
     3841 gcaccagttt agtgggagag tcgcatttga tcagcaaggt ctatttccct cgtctcatta
     3901 ttccgctcgc cgccgcatct gcacccgccg ttgatctgct ctgcgcgttg ctgatgatga
     3961 ttccgctcat gttatggttt ggcgtgaccc cgggttggca gttgctgttc ttccctgttt
     4021 ttgtgggaat tgccttgctc gccgccctgg ctgtgagttt ctggttttct gcgttacatg
     4081 tgaaatttcg cgatgtcgga catatcatcc catttttcgt gcagttttgg atgtttgctt
     4141 cgccagtcgt ctatcccgcg agtctcattc ctgaaaaatg gcgcaccctc tacagcctga
     4201 atcccgcggt gggagtcgta gagggttttc gatggagctt gctggggcaa cgtcctccct
     4261 cactcgaaac gattctcccg agcgtaggca tcgtcctggt tttgttcgtg agcgggatcg
     4321 tttactttaa acgtatggaa cgcactttcg cggacgtgat ttgatggctg agatcgcaat
     4381 ttccgcacgg cacgtgagca aagaatatgt catcgcagag atcaatcatg acactttgcg
     4441 ggatcgcatc gctcatggct gtcggtctct cctgggacga cgcgctgcga agagtgtacc
     4501 cccaaaatcc ttccaggcat tgaacgatgt ctcattcaat gtcatggaag gggaggtcct
     4561 cggggtaatc gggcataacg gggcagggaa aagcacgctg ctgaagattt tgtcccgcat
     4621 taccgaaccg accggtggag aagtggatct atacgggcgc gtcacatcat tactggaggt
     4681 gggcaccgga tttcatgcag agcttacggg ccgagaaaat gtgtacctca atgccgctat
     4741 gctgggcatg cgcaaggtgg acatcaataa gagattcgag gccattgtcg aattctccgg
     4801 gacgcagcaa tttatcgaca cgcctgtcaa acgctattca agcggtatgt atgtgcgact
     4861 ggctttttca gtggcagcgc atcttgatcc agagattctg atcgtcgatg aagtgctctc
     4921 cgtcggcgac ggtgcttttc agaaaaagtg tttggggaaa atggacgagg tacggagaga
     4981 cggacggacg gtcattctcg tcagtcacga tttgccggta gtggcgagcc tctgtcagcg
     5041 ggctatcctg ctgcggcatg gccgggtcgt gggtgatggc caaccgcaac agatcattga
     5101 gcaatatatg agcgaggcat tcacaggtac cggagtctcg ctagccgatc gcaccgatcg
     5161 ttacaccaaa ggcgaactga cggtgcaggc gattaccttc ctggacgaca aacttaaccc
     5221 tctgcagccg gcattatcgg ggcaacatct tgtcattcgc gtgcagtata agtgtcatgt
     5281 ccggaaggca tttaaggata tgcgtgtcat ggttgtcatc aatcgtgatg agcgcacgtc
     5341 ctgtattttt tcgacggatc ttgtgagtca tacccctctg gatttggagg gcagcggcta
     5401 tgtggacttc catgtgccgc atctgccgtt atgcggcggc cgatactttc tccatgccgc
     5461 gattgaaaac aataccatga cgcaggattg ggttcagtat gcagctgagc tgcaggtctt
     5521 agatggcgac tattacggga ccggccgaat gtatccgcat gacggatggc ggggaaaagg
     5581 catgttcgtc gaccacagtt ggcgatttga aagaacctcc tgactagggc ctagcgacga
     5641 cctgaccctc tctttttccc tcgtgccatc cgccacttcc atcagttcat cgcgaaagtt
     5701 tcggttttcc cccttagagc cagtctgttt cgcgatgaaa cattcctccc ctggggctac
     5761 gttgccgtcg accaatccat ggtcctgatc cattcaggca gggccgtgat agggccgatt
     5821 gtcaggggtt tacctgattt gttctggggg gtatcttacg catgttttag cctgtgaatc
     5881 gcgaatcggt gtcgatgtga gtctggtggg agcgattcga gcatgttggt tgccatctgc
     5941 ctgctgactg ccggtgtctt tgccctggat gtcgcgatgt cgctagggca tgtcatttgg
     6001 ctgttgtatg cgcttccgct ctggttgagc gtgcgattga cctcgcccgc agcacccctg
     6061 cgctatgcct ctctatgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     6181 gtttgagttg gcagcggaat gcgcagatgt gggcgtgtgg gaatgggatt tggtgaccaa
     6241 gtcggcctat tactctcctc ggtggaaaac tcagcttggt tgcacggaat ccgacattgg
     6301 tcccacggtc gccgactggg agtcgcgtct gcatccggat gagcgggcac aggttcttgc
     6361 gacggtcacg ctgtttcagc agggttatac caagcagtat cgcttggacc accgccttcg
     6421 acaccaggac gggtcatatc gatggatggc ggcattgggc gcgggcgttt atgacgagca
     6481 ggggtgcatg atccgcatga cgggcatcca cctggatatt acggtgagaa aacaagctga
     6541 ggaggcccga agggagagcg aggagcgcta ccgggagtta gtggaacagg ccggggatat
     6601 tatttatcgc accgatgctg ccggacgatt cacgtactgc aacccgacct cattgcggat
     6661 tctcggctat ttcccggagg aactgctcgg ccgccattac ctggacattg ttgagccgca
     6721 gagtcgtgcg caggcagagc gtttttacgg acggcagttt atccgcaaaa cgctccggac
     6781 cgtgtacgag ttgccggtgg tgaccaaaga tggtcgggaa gtgtggctgg ggcagcacac
     6841 acaactgttg atggagggaa atactgtcca cggcttccag gtggtggcgc gggaagtcac
     6901 cgagcgaaag cgagtggagc aggcgttgcg ggaaagcgag gagcgttttt ccaaggcgtt
     6961 tcatagcagc ccggcaggga tggccatcag ccggcttaaa gacggacggg tacttgatgt
     7021 caacgaggcg ttcgtgcgtg tctcgggatt ttctcgagaa gagctgatcg gcatgtcgtc
     7081 actcgacatc ggcatctgga tcaatccgga cgaccgacgg cacttggcag aggctttgcg
     7141 gcgggatggc tttctccggc atctggaaaa agagttccgt acgaaatccg gcgaactccg
     7201 gcacggtctc ttcaatgtgg aaccggtctg tatcagtcac gaaaactgtg tgctgacgct
     7261 agtgctggac atcaccaggc gcactgaggc agaagcggca ttgcgccgca gccaaacggt
     7321 gctccaagcc caccagcatg cgttgatgcg tctcacaaaa agccaacata tcggctcggg
     7381 ggactggccg tccgcgctga aagaactgat gcagacttcg gcggaggcca tggctgtcga
     7441 tcgggccagt gtctggttat tggatcgcac cggggcggta ttggagtgcg tagagctcta
     7501 tcaacgaaac gaggcgcggc actcgcgggg ggcacgtctg gacgtggcgc agtatccgcg
     7561 ctactttgcc gagttgctgg aggagcaagt cgttgatgcg catgatgtgg ccagcgatcc
     7621 tcggactgct gaattgctgc agccctatct tcaaaccatg gggattgcct cgttgttgga
     7681 cgtgccgatt ttcttcggtg gacgcctggc cggggttgtg tgccatgaac gtctcggctc
     7741 ggcgcgaccg tggagcaatg aagatgcgca gtttgccacg tccatcggga atttggtcac
     7801 gctggcctac gagtcgaagc aacggcgtga ggcagagagc gccctcgtta ctgcgaagga
     7861 ggcggccgaa tttgccagtc gggcgaagag cgactttcta gccaccatga gccatgagat
     7921 ccggacgcct atgaatgcga tcatcggcat ggccgatctg ctgtcggaaa cgccgctcag
     7981 cgaagaccaa cgggagtacg tccagatttt tcgggacgcg gggggaaact tgctgagttt
     8041 gatcaacgat gtgctcgatc tctcgaagat cgaagccggt catctggatt tggatctcac
     8101 ggacttcgat ctcagtgacc tggtacagcg cgcagccgaa ttggtcgcag tgcgtgccag
     8161 cgaaaaaaat cttgagctgg cctatcagat ccaaccggat gtcccgacct cactcgtagg
     8221 ggatccgaat cgcctccgcc aggtactgct gaacctcttg ggcaatgcga tcaagttcac
     8281 agatcaggga gaagtcgtgt tgcgcgtcga atgcgacccg cacgcagagg ggcccggcag
     8341 tctgttgttc accgttcggg ataccggcat tggaattcct cgagacaagt tggccgtcat
     8401 ctttgaacgg ttcacccagg tggattcttc gatgacgcgg ccgtatagcg gcactggtct
     8461 cggtctcacg atttcacggc gcctcgtgga gcgaatgggc ggcagaatgt gggtggagag
     8521 tgaacccggc cgcggcagca ccttcagctt tacggccaag tttgctgcgc acgcgcaacc
     8581 ggcctcaccg gtccccacgg cacaatggga gcaacttgcg gggttgcgga ccttaatcat
     8641 cgacgataat gccaccaatc ggctgattgt gcgggaaacc ttgagtagct gggggatccc
     8701 ttcggcggaa gcggccgggg gagacgaggc attgctggaa ttgcgccggg cactcacggc
     8761 cggcatgcct taccgtttgg caattctcga cgtgcgcatg ccgaaactca gtggatggca
     8821 ggtggccgaa accatcgcac gctcgcccgg tctggccggc ctatcaatta tcatgctgac
     8881 atcagaacgg cgcgccggtg accaggcccg agcccgtgaa catggagtcg tgcggtacct
     8941 cacgaagcct tttcgacgct ccgatctgtt caacgcgatg atggccgtgt tgggcaagac
     9001 agcgccagcc gggaatcctg aatccgaagc tccttctcag ccgaaattcg ctggagatga
     9061 ccagtcaggc ctgaagattc tcttggcgga agatttcctc gacaaccgcc gcatgatgga
     9121 gttttatttc aaggcaacgc cgcatcgggt ggcgacggcg gccaatggtc agatcgcgct
     9181 cgagatgttc atgcacggat cgtatgatct ggtcctgatg gatattcaga tgcctgtgat
     9241 ggacgggtat gccgcgacga gggccatccg cgcatgggaa aaagaccaac gccgcagatc
     9301 cacgccgatc ctggcattga cggccaatgc gctgcagagt gaggtgcagc ggagtcttga
     9361 tgcgggatgt acggcgcacc tcacgaaacc cattcgtaag gctcagcttc tagaggcgat
     9421 tcaactctat ttccagacag cctcctccca gccgccagtt cccgcatacc acgaagaacc
     9481 tgccgtgctt catgtcaacg tcgagctgga agccctcatg cctgggtttc tagagcatcg
     9541 gcgccaggac ttgatccaga tgacggcggc attggatcgt gaggattttg aggtgatgcg
     9601 ggaaatcggc cacggtctca agggagccgg cggaacatat gggcttgaag cgattacagc
     9661 gtatggacgg tccttggaaa cggcggcctc gagaaaagat ggtcccgcgt tacgcgaaat
     9721 cctggaacgg ctccgccggt ttctggatcg gttgcaactg atctacgtgt aaaggataga
     9781 cggtatgcgc gtattgctgg ttgaagatca tcccgatgtg cgactcctgt tcgagcaggt
     9841 catcggggcc cgaggacatg aggtgacggc ctgcgctgat ggcgaatcgg catgggaggc
     9901 ctacggcaag cggtcatatg aactcgtgct gctcgattgg gaattgcgtg gtggaggaat
     9961 ggacggattg cagctctgcc ggaggattcg ctccggtccc ggcggagatc gctgtgtcat
    10021 cgtcatgatc accggacacg attccccgga atccttgcgt gcggcgctgc aggccggcgt
    10081 caacgactat ttagccaagc ccgttggtgt cgagttctta cgattgcgat tgacgattgc
    10141 cgaacagtgg gtcgaaagtg tgcggcgccg ctttgcagct gaagatcagg ctcaggcgct
    10201 gcaatcgcaa ctggcggacc acggcaagtt tcatgatctg atcggccgaa gtccgtctat
    10261 ggtggtcttg tacgaggaga ttcaacaaat tgcggcagtg gatgcgaccg tgttgatcga
    10321 aggcgaaacc ggcacgggca aggaactggt ggcccgggcg atccatctgt ccagccggcg
    10381 cgcgccgcac acatttcttg ccgtcaattg cgcggggttc accgactcga ttttgggcag
    10441 tcagttattc ggtcacaaaa agggcgcatt taccggcgct atcgacaacc aggaaggtgt
    10501 ctttgaggcc gcgcaagggg gcaccatttt tctggacgag atcggcgata tttcccctgc
    10561 agttcagacg agcctgctgc gcgtgcttca ggagcgggaa gtcacgagac tgggagaatc
    10621 caagcctcga aaagtcgatg tccgtgtcgt ggtcgcgacc catcacaacc ttgtggaaga
    10681 cgtcgagaaa ggcacgttcc ggagggatct gctctatcgc atcaaggtgg ctcgccttca
    10741 gctgagtccg ctccgtgaac ggccgacgga tattccgctc ttggtccatg cgtttcttgg
    10801 tcaatttcgt tcggtgatgg aaaaaccggt gctgcatgtg agtccagagg cgctgcaggt
    10861 cttaacgagc tattcgtggc ccgggaatgt gcgggagttg aagagcgccg tcgagtcggc
    10921 cttgattcat tgcaaaggga ccgtcgtgcg tccggaagat ctccccccgg agatccggag
    10981 cccgcaacca caggccacct atgttccgct ccagcgacaa gacgaacgaa cccgcatgct
    11041 gggagccttg cagcagacgg gcggcaatcg ttcagaggcg gctcggttac tggggatgag
    11101 ccggagaacc ttttatcgtc gcctggctga atacgatgtg gctgcctcgg cggagcccgg
    11161 cgcagagtcc ttcctgtgaa ctggtgagcg tatgcgcacg cgcgcttcgt tgctctcaag
    11221 ttgccctcaa ttggacgtat ttcttaataa cgtcatgcac cgttcacgct gatctccttt
    11281 cggtttccct attgcaagtc tgcgtgttcc gcacatcact gtgcatagag tcacatgaca
    11341 cacgtgtgac acagcacagt gtgccatctc attgacgcat gcgggtgttc accgacgtgg
    11401 tttcgggcct aagccccagt atttcgtgga gcttgttgtt ccttcatcga tggcacgatc
    11461 cttgatacat gatctccacg aacagcgtag tggagagaaa tggtggaaca gcaagggccg
    11521 agtcagaaag agggcagcat ggaggttgaa ggatttctcc tgtcgaacga tatggaccag
    11581 gaggtcttgg tgaccattcc gccttcatct gtcggcccat tcgatcaacc agccgtcaaa
    11641 gagcgtattg atggatattg gatcgagatg tctcgattgc cgaacaggag atggtctgac
    11701 cggacgcgtg cggtcggagc cgtgctgacg tacttgcaag gcttgtcggc aatttcgtca
    11761 gggtttgcta cccacacgag atgacgagaa gctgcaacca gaaccaaagg agtacagacc
    11821 atgtggaatg accagaatct attgcggagt gcccgggaag acaatgcgcg ggatggagca
    11881 gccggactga gtgtggtgcc gggcgtgagt ctcacggcga aacgtcgccc tgaagatttg
    11941 accccgcgcg aacaggaaat tctgcagctg gtctgggcgg gattgaccaa tcgcacgatt
    12001 gcggaacaat tgcacatcag catcaagacg gccgaggcgc atcgggccaa tatgatgaaa
    12061 aaactccggg tctccaatat tgcgcagcta ttgaaaacgg cgctggaaga ggggctgctc
    12121 gccacccacg cgggctgaag ggattcgcgg cggcctgcac tctcggggga agtcccactt
    12181 ccggcagtgc atcggctccc gcgagtccgc cccctcacag aacaaatgag gctcgcaccg
    12241 gcgacggcgt ttgcagtaga atgccgcacc gtcgctggag cgagccgtgg tcgaacaacg
    12301 catcgaagaa attacccgcg ccaatctcgc cttttatgcc gcctttgaaa gtctggatat
    12361 gctccagatg gacaaggtgt gggcacatct cgaatacgtc acctgcattc accccggctg
    12421 gacccttcgg agcgactggc ctgccgtacg cgattcctgg gtgttgatct tcaacaatac
    12481 cttctccatg aaattcgaac tatccgacgt gcaggtacag gtggcgggcg atctgggatg
    12541 ggtgatctgc accgaacacc tcacgagcag gcaggacgac cagccggtgg aaacgcgcgt
    12601 attagccacc aatctctttg aacggatcgg tgatgagtgg ttgatgatcc atcaccacgg
    12661 cagccctgtc atggggtagg gggcaatgag tgcggaggcc ggatcctgtc tactggccag
    12721 gcacctctgc ccgcacgggg gctacagcgg tggatgccga tgctcggcgt gtgaatggta
    12781 aacgttcctg catgacaccc ttatctctcg ccgtcagatg gacaggcttc tgcgcccttc
    12841 tgttgtccgg atgtaccacg gtggctcaga ttacaaccct gtcggatgag tcctgtcggc
    12901 aaactgtgcg cggtcaactg gaatccattc tcgttgaaga gggagaacga cccgagatgg
    12961 caactcgtct cgcggtcaac accaccgtgg tcttggcaac cggctctcta gggcctcgcc
    13021 cgttcggagt ctcgtcgccc tctggcaccg attacagttt ctttgtccag cgtaagggtg
    13081 aaagctgcct gctgcgtctg tatggacgtc aaaagggatt cacacgctac accaacaatc
    13141 tcacctatat ttcgactcgt cctctggaag gttgtgcctg cgccgaatga ccggggctct
    13201 gactgtggtc ttcttcctct cgtccatctc ctgcttctga actgaatcct tgacccggtg
    13261 tgtcatgggg gctggcgctt ccggcgagcg gcagcgcggt gtcctattct gtctcgtcac
    13321 ctgtctcatc ccgccaatgt ggcacagctc tgtgaatcat gacacagcca gtgatgaccc
    13381 tcctttcaaa agataatttc gcaagtcatt gtttaatcag caatggcagc cgcattgtgg
    13441 gcatggcacg ggccttgatg tagtgctcgg tagatgtcgt aaggcgaaca ccttggggag
    13501 ttcaagggag gagggaagca atggaagtga aagagcgtcc acgggtgagc ccattgtgca
    13561 ggaatcacaa ggaggaacga tccatgatgg tgaaagggtt tttgctgaaa gatcgggaag
    13621 agcaggaata cttgttggcc attcaaccgg cgacgcatgg gatgttcgag cggctcattg
    13681 tacgccagaa gattgatgga tgctgggtcg aactcaatgg caatgcgcag gggacctggc
    13741 agccgcgcac gcaggccatc acggccgtgt tggggtacct tcaacgcctg tgggatacgt
    13801 cgaaaccggt cgcagctcgc gagaactagt ccactgacag ctcgtcaccg tcagtgccgg
    13861 tgagagacga cgaaggagga ggaccatgca ggccgaaaag caagacaggg gcggggacac
    13921 gatgagcgga tcgtccatga agagcggcga gatgtctgcc gatcctgacg cagcttcctt
    13981 gaagcgcaag cccgaagatc tgacgcctcg cgagttggaa attctccgtt tgatctggga
    14041 tggtcacacc aatcgcagca ttgcggagct gctgaacatc agcatgaaga ccgccgatac
    14101 ccatcgggcc aatatgatga agaaactgcg ggcgtctaat acggctcagt tgctgaaggc
    14161 ggcaatagaa gggaaattac tgcacatcca cgagggggcc cggcgttccg acggcgcgaa
    14221 gtgattactc atgcctatcc gcaagcgtcg ccggcttcgc ctggtgaggc gacgcgggaa
    14281 ggcacttcga gagtcagtgc aacctgttca caatccaggg atggggtggg gaagatcttt
    14341 ggtgccgagt gtctgaagcc actcacctgc gatgacatca cgttcttcca tcgtcatgcc
    14401 ggcatacttg cggaacatct gggggccacg tctccgtcgc caggtcagaa atcccaggaa
    14461 gtgatccttg cagatagaac gggtgcctgc cggcgcatac actctcgcct gacaaccat
//
LOCUS       DAOQEW010000088        14481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1174_length_14481_cov_8.022598, whole genome shotgun sequence.
ACCESSION   DAOQEW010000088 DAOQEW010000000
VERSION     DAOQEW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1174_length_14481_cov_8.022598"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PLS35_10690"
     CDS             <1..1030
                     /locus_tag="PLS35_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19176.1"
                     /translation="QVVPVLNALARLVPDLKALLRTSVPSGFFEPRLTIPWQLSPAQQ
                     DIGCIQNGPLTIDVAGTWSAHRTLHADWETKIEAEASLIRQASPRLVLSDVSHLGIAA
                     GAAAGIPTIGLCSLSWDLVLEPFVDPAEPDHAVIMRQIREAYANADCFLRVAPGLAVT
                     AFRQMRDVGPIAEPSGPQTATLRQAVGATPSEKVILVGFGGIALKTPPFEHMEAMHSF
                     RFLFDGPVPAGLSRVSSLSAVGLPFKHILASVDLVLTKPGYGTTVESVALGIPVVYVR
                     RYNFADEQSLVDYLQRHGRSAELSLDAFYQGHWEPAIREALAQSPASPPPPLSGAHDA
                     ANILHTYF"
     gene            1154..1759
                     /locus_tag="PLS35_10695"
     CDS             1154..1759
                     /locus_tag="PLS35_10695"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI19177.1"
                     /translation="MESAPRQAASTIEPKLLARVAKGDQAAFGQLYDQSSTLLFTLAY
                     RILNDRDEATELLQDVYLEVWRKIAKYDVGRGSPIAWLVTLTRSRAIDRLRSRASRGQ
                     HVVADSFEHPLVSVTPDVSPNPYEAREDTEMRQMMAKAILDLPLPQQQAIEMAFYQGL
                     THTEIAAKLNQPLGTVKTRIKLAMSKLRASLQETLPQGHQL"
     gene            1756..2772
                     /locus_tag="PLS35_10700"
     CDS             1756..2772
                     /locus_tag="PLS35_10700"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNI19178.1"
                     /translation="MTHEELEEAVPLYAIGALERSERQAIEAHLLSGCAACHAALKDY
                     QTVASLLPFGLTPATPPNTLKAKILMAPASTTSQPETEQPGPRSSLEPGEWMNHLFPP
                     ITPARSLPFRIAMGFAAFVVVVGGGYLAWMNYTQTAQRSGEIQQLQAAVQRETSRASS
                     LQSELRQREQTLDSLKTDLDQRTTEVAELRDQLLQREAELDDAHQQLTQHDSSLQRLA
                     QQSETFVGLFKNPTSKVVLLSGSDMAKSAGAFLLIDPASKKAWLYAFNLPALPNGKVY
                     QLWAIDDKPVSAGVFGLDAGQKARMLIKNMGEFPRMKKFAVTVEPDGGRPEPSGAIYL
                     IGQI"
     gene            2826..>3142
                     /locus_tag="PLS35_10705"
     CDS             2826..>3142
                     /locus_tag="PLS35_10705"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNI19179.1"
                     /translation="MPIVRTRCVLSLTGITHDTMSLPDDLDRQFMQEALSLAREAPLI
                     GEVPIAALLVRDGVVIARAHNLRETVQDPTAHAELLVIREAAKQMNSWRLTESTLYVT
                     LEP"
     assembly_gap    3143..3242
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3243..3888)
                     /locus_tag="PLS35_10710"
     CDS             complement(<3243..3888)
                     /locus_tag="PLS35_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNI19180.1"
                     /translation="MMSRAPDPICASLSFPHLDLTSVHVWCCELPRYAAVQESFAAQL
                     SEQERTRAARFAFERDRQRFVLSHGVLRQLLARYTHIKAGEIQFITGRHGKPALAVPS
                     VPSENIQFSLSHSGEYALVAVARGREVGVDVEVFKANMDCAKLAERFFAPREAQAMAT
                     LCGEPQQRMFYRLWTAKEAYFKGLGVGLSLGLERCEIVFDGESPHARVRLADSGT"
     gene            complement(3917..5614)
                     /locus_tag="PLS35_10715"
     CDS             complement(3917..5614)
                     /locus_tag="PLS35_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HNI19181.1"
                     /translation="MMKLYRFLLFLLRDARTMMILMVMTGLLAGLSSVGLLAVINKLI
                     NGAGTTSELFALAFIGLAVLKVGSNYLSQLLLVTFAQKTILKLGMDLCWKVVRAPYRT
                     LERRGSHEILATLTDDTNALAWAVNGLPGLAINVAILGGCSLYLAWLSWQAFLGVVIL
                     AVLGLLGYRQLYNRVLQSSLAVRDSKGVLFEHFRSLTEGMKELMLHRGRRETFVEEDI
                     RQAADALRRHNLTTTKQYLVTDSWTQVLFYGLIGVILLLFPRVLSLSGESLTGYAFAM
                     LYMIGPMWGLLGMVPTLSRGQVALEKIESLGLALDEGGREGGAERPMAQGRPCVEFSN
                     AVFAYESKGEDERSFRLGSLDLSIRAGELVFIIGGNGSGKSTFVKVLTGLYLPQQGQV
                     TLNGEAITSATQDWYRQHFAAVFSDFYLFKKLLGLDPSLVASQADGWLKTLRIDHKVR
                     IEQGEYSTVNLSQGQRKRLALVTAMLEDRPFYVFDEWAADQDPQYKDVFYGELLPNLR
                     ARGKGVIVVTHDDRYFHVGDRVLKLDDGRIVDVSTGAAFNATQRPPTLLKPVARSSA"
     gene            complement(5668..>11376)
                     /locus_tag="PLS35_10720"
     CDS             complement(5668..>11376)
                     /locus_tag="PLS35_10720"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HNI19182.1"
                     /translation="EPGVVVGLCLERSLNMIVGLLGILKSGGAYLPLDADYPTERLEY
                     MLRDSQVRVLVTQQDQLVRLPATNPHTICLDSEWAQIAKCPDSPIDVTDALDNLAYVI
                     YTSGSTGHPKGVMIEHRSIANYVRAITKIVDLTGEDRVLQFASLSFDTAAEEIFPCLA
                     AGATLVLRTATMVDSVSGFLDRCRELTVTILDLPTAYWHELVTRMALEQVAFPSSIRS
                     VIIGGERVLPQIVQRWTDLIGATVRLINTYGPTETTVAVTWSDLTGLEAHDELIGDLP
                     IGRPIPHTSVFVLDRNQQPVPVGVAGELYIGGVGVARGYRGRPDLTETQFVPDPFSKQ
                     PGARLYRTGDLVRWRPDGQLDYRGRADRQVKIRGYRIELEEIEAVLNRHQDLERAVVE
                     VREDQPGDKRIVAFMVPRPNNRLGLVQLREQLRSQLPAHMIPSTFIEMETLPLTVNGK
                     VDRKALQVAADSRASKVELTSQYLAPRTPTEQVLADIWGEILQLKDVGVHDHFFELGG
                     HSLLATQLVSRVQSLFRITLPLRQIFERPTIATLAEVITQAQAGANSHQGESGYVITR
                     ARRGIPLPLSFAQERMWFLYRLSPDAVAYNIPASVRLHGPLNKPALRWAVAELVRRHD
                     ALRTTFAHVDGQPRQIIQDVLDPTWTEEDLRGVPAELREPRALELATAEARRPFDLEQ
                     GPLLRVFLIQLGDEDQIVILNTHHIISDQWSYGIIARELVTSYNAFCAGRPFAVQPDL
                     AIQYADFAQWQRGWLTGPVLEEQLAHWKTKLVDLPVLTLPTDRPRLPVHSFKGDHVSL
                     DLSWSLINRLKQLSVREGVTLYMVFLAGFFGLLHRLTQQKDLVIGTPIANRNRLEIEE
                     LIGTFVNTLVLRADVTGELTFRDLLRKVRDLSLDAYAHQDIPFEKLVEELRPDRSQGG
                     LPLVQVLFNFANTPFARTEFHQLSWTPYEVSRGAAQLDLGLSIDPYASRKAYLEFNTD
                     LFDRASAERWISQYRQLMETVAEHADAELGRVSILTGQEQHRILREWNATDRPVDQGL
                     CFYQLFEAQVSRTPDAVAVRFEGREWSYAELNRRANRLAHRLRECGVGPDAVVPVFLE
                     RSPDLLISLLAVMKAGGAYLPLVPGLPIRRLAAMIEASHAAVLITESALLGGLPQHQL
                     PVLCLDQEAEVLARQAEENPSPRSGPEHLVYVLFTSGSTGQPKGVEIEHRALVNFLRS
                     MQQEPGITHRDVLVALTPLSFDIAGLELYLPLLVGATIILANRQQAMDGAWLQRELDH
                     GAVTMLQATPATWRMILQFGWKGGRGVKVLCGGEALPRELAQELLARAGSVWNVYGPT
                     ETTIWSTLERVRNAERTISLGRPIANTQVYVLDGNLEPVPVGIPGELYIGGMGLARGY
                     RGQSQLTAERFITNPFKAGERLYRTGDQVKWLPDGRLDYVGRIDHQVKLRGFRIELGE
                     IESVLGNDATVKQAVVIVREDSPGDKRLVAYVAPREGSACDPSSLRRALREVLPDYMV
                     PAAIVPLTEFPLTPNGKVDRASLPAPAAEPVHDGAQAIEPRNRVELQLVAIWEQVLGI
                     TPIGVRDNFFTLGGYSLLALRMFSAIEQTFGTRLPMAVLFQAPTIEQLADVLADEGCT
                     VRWRSLVAIQPEGKRPPLFAVPGVGGNVLVFARLAKLLGDDQPFYGLQARGLDGKEKP
                     FMRVEEMAAHYIEEIRSVQPHGPYFIGGTCTGGLAAYEVAQQFTAQGEEVILAVMESW
                     HPRSYLNHWSRPPYLLWPLLFVWMKVTTYLRLMRRLPADEWASFWKGKLKRMWNLMHH
                     TEAAEHQDEFLYKDQVTYATFHAVARYELKPFRGQVLNVIASKHPLTNSSDDTRLVFG
                     EWAMGMSRTVYLPAEDSGRLFVAPHVQELARQLKAFWQEAEAVYRKRPDGQGDGPTTK
                     AA"
     assembly_gap    11377..11476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11477..14460)
                     /locus_tag="PLS35_10725"
     CDS             complement(<11477..14460)
                     /locus_tag="PLS35_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNI19183.1"
                     /translation="MVRHPPVTIPAGDTLLDVLRWRAEHQPDQTAYVYLREGLSADSV
                     LTYRDLLTRARSIASYLQARFAPGDRLLLVYPPGLEFVQAFWGVLYAGLIAVPVPPPD
                     AFRLKAGVARVQRLAEDAGAAGALTTAQMLETLRSQELVASLHQWVSSDQALSADSSG
                     WTDLQQLPTHLAYLQYTSGSTSAPKGVMVSHGNMTAQSRCITDAGGYDAGSVTLSWMP
                     HFHDYGLVKGIIQPAWIGRPSYLMSPLTFLKRPLRWLEAIQRYAVTHSGAPNFAYRRC
                     VEATTPEERAGLDLSQWQVASCGAEPIAPDTIQRFAAAFAPSGFQREAFFPAYGMAEY
                     TLLISLKRRGAAPTVTTLDAAALEKGAVIEAVADDKPVRKVVGCGMPVGDTRVVIAHP
                     ETRCRCAVQQIGEIWLSGASTTQGYWNKPDETSHTFGASLADTGDGPFLRTGDLGFVK
                     DGEVFVTGRFKDLLIVRGRNHYPQDLERTVESCHGALRAGGAAAFTVEEAGEETVVLV
                     QEVERQASPCSTEDLAAAMRAALSEQHDLHASTIVFIKAGSLPKTSSGKVQRRACREQ
                     YLAGRLSIIGTSVAPHPVAQSSPAPIHTDAWQRLSGEARTSAILQTVQKLLADRLGCM
                     PQAIAVDRPIHLLGLDSLMAADVLHRLEESFHLSLSLSALLGGATPADLTTMIERGLA
                     GESRAESVAAGADPAIATVFPLSENQAALWFLSQLDPDSAAANVSVLLPLPSDLKADR
                     LRAALEAIGDQQAMLRTTFETQEDGPVQRIHDRMPLSWSEIDSSTWEWPQLRGESVKA
                     AAVPFNLTQGPLWRATLFHGGQQSWLLLVAHHIVVDGWSMMQLVDDLKRRCEVAGSCS
                     SEYSPEPQDRPAPYREFVEWHRAILEGEEGSRLGQYWKAKLAGELPNYDMLYDRPEIT
                     VEPSHYAWHAFHIDEDLTRRLKKFARTEGTTLYAVCLSALQILLTRYTAQEDTAIVTP
                     VFGRSRARFARTVGDFVNMLVLR"
BASE COUNT         3053 a         4530 c         3984 g         2714 t
ORIGIN      
        1 gcaagtcgta ccggtgctca acgcgctcgc tcgtctcgta cccgatctca aggcgctcct
       61 ccgaacatcg gttccctccg gattctttga gcctcgcctg accattcctt ggcaactcag
      121 ccccgcgcaa caggatatcg gatgcatcca gaacggcccg ctgaccatcg acgtggcagg
      181 cacgtggtcc gcccatcgaa ccctgcatgc cgattgggaa acaaagatcg aagcggaagc
      241 ctcgctcatc cgccaggcta gcccccgcct ggtcctgtct gacgtgtcac atctaggcat
      301 tgccgccggc gcggcagccg gaattccaac gatcgggctc tgttcgctct cgtgggacct
      361 ggtcctggaa ccgttcgtcg atcctgctga accggaccat gccgttatca tgcgacagat
      421 ccgcgaagcc tatgcaaacg ccgactgttt cctgcgcgtg gctcccggtc tggcggtcac
      481 cgcgtttcga cagatgcgcg acgtgggccc gatcgcggaa cccagcggac cgcagactgc
      541 caccctccgt caagccgtgg gagcgacacc atccgagaag gtcattctgg tggggttcgg
      601 tggcattgcg ctgaaaactc ctccgtttga gcacatggaa gccatgcaca gctttcggtt
      661 tctctttgat ggtcctgtgc cggcgggact ttcccgggtc tcgtcgctct ctgctgtcgg
      721 actgccgttc aagcacatcc tggcatctgt cgatctggtg ctgaccaagc ccgggtatgg
      781 gaccaccgtt gaatcggtcg cgctcgggat tcccgtggtc tacgtacgtc gctacaattt
      841 cgcagacgaa caatctctcg tggattacct gcagcgacac ggccgatccg cggaactctc
      901 tctcgatgct ttttatcagg gacattggga gccggccatc cgggaggccc tggcccaatc
      961 acctgcatcg ccgcctcccc ccttgtcagg ggctcacgat gccgccaaca tcctgcatac
     1021 gtatttctaa acagacgccc cattccagcc gcgcacgacg cacaaactta catctgatta
     1081 gtcgtttttc tcagacagtg ttatagtggg cccaattctg atcccgagga ctgtgcgcgc
     1141 ctaggccgac tgcatggaat ccgctccccg acaagccgca tccacaattg aacccaaact
     1201 cctggcccgc gtggcgaagg gggatcaagc ggcattcggc cagctttacg atcaatcctc
     1261 caccctgctc tttaccctgg cgtaccggat cctcaacgat cgcgatgagg cgacggaact
     1321 gctccaggat gtgtacctgg aagtctggcg aaaaatcgcc aaatacgatg tcggtcgagg
     1381 aagccccatc gcctggctgg tgaccctgac ccgtagccgt gccatcgatc ggctgcgctc
     1441 gcgtgcctcg cgcgggcagc acgtcgtcgc cgattcgttc gagcacccgc tggtctccgt
     1501 cacgcccgac gtgagcccca atccatatga agccagggaa gacactgaga tgcgccaaat
     1561 gatggcgaag gctattctcg atctgccgct tcctcagcaa caagcgatcg aaatggcttt
     1621 ttatcaggga ctgacgcata cggagatcgc ggcgaaattg aatcagccgt tgggcaccgt
     1681 caagacgaga atcaaactgg ccatgtcgaa acttcgcgcc tccttgcagg agacgctgcc
     1741 gcaagggcac caactatgac ccacgaggaa ctggaagaag cggttcccct ctatgcgatc
     1801 ggcgccctcg aacgctctga gcggcaggcc atcgaggcac atctactctc cggctgcgcc
     1861 gcctgtcatg ccgccttgaa agattatcaa accgtcgctt cgctgctgcc gttcggactg
     1921 acgcccgcaa cgcccccgaa cacgctcaaa gccaagatcc tgatggcccc ggcatcgacc
     1981 acaagccagc ctgaaacgga gcagccggga cctcgatcca gcctcgaacc gggcgaatgg
     2041 atgaaccatc tgtttcctcc catcacgcct gcccgttccc tgccgttccg catcgccatg
     2101 ggatttgcag ccttcgtggt ggttgttgga ggcggatatc tcgcctggat gaactacacg
     2161 cagacagccc agcgctcagg cgaaatccag cagttgcaag ccgcggtgca gcgcgaaacc
     2221 tcccgcgcgt ccagtctgca gtcggaattg cgccagcgcg aacagacctt ggattcgctg
     2281 aagactgatt tggatcaacg cacgactgaa gtggccgagt tgcgggatca attacttcaa
     2341 cgggaggccg agctggatga tgcgcatcaa caactcactc agcatgacag ttcattacag
     2401 cggcttgccc agcaaagcga aacatttgtc gggctgttta aaaatccaac atcgaaagtc
     2461 gtcttactat ccggctccga catggcgaaa tcagccggcg catttctcct gatcgatccc
     2521 gcgagcaaga aggcatggct ctacgccttc aacttaccag cgctcccgaa cggcaaggtg
     2581 tatcaactct gggccatcga tgacaaaccg gtcagtgccg gggtgtttgg cctggatgca
     2641 ggccagaaag cccgcatgct gattaagaat atgggtgagt ttccccggat gaagaaattc
     2701 gccgtgaccg tggaaccgga cggcggacgc cccgaaccgt cgggggccat ctacctgatc
     2761 ggtcaaatct agcctcagtc cctccaacga acgtcgcctg cgattcccgg cccaaacatg
     2821 ctagcatgcc catcgtcaga acccgatgcg tgctctcgtt gacgggaatc actcacgaca
     2881 ccatgtccct gcccgacgac ctcgatagac aattcatgca agaggccctc agccttgcgc
     2941 gggaagcccc gctgatcggc gaagtcccca tcgccgccct cctcgtccgc gatggcgtcg
     3001 tcatcgcccg ggcgcacaat ctgcgggaaa ccgttcagga ccccacagca catgcagagc
     3061 tgcttgtcat ccgtgaagca gccaaacaga tgaacagctg gcgcctcacc gagagcaccc
     3121 tctatgtcac cctggaaccc tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnaggttccg gaatcggcca gccgcacacg cgcgtgaggg gattccccat cgaacacaat
     3301 ttcacaccgc tccaagccga gtgacaatcc cacaccgagc cctttgaaat aggcttcctt
     3361 cgccgtccac aatcgataaa acatgcgttg ctgcggttcc ccgcatagcg ttgccatcgc
     3421 ctgcgcttcc cgcggagcaa agaagcgttc ggccagtttg gcgcagtcca tgtttgcctt
     3481 gaacacctcg acatcaacgc cgacttcacg gccgcgagcg acagctacga gagcatattc
     3541 tcccgaatgg gaaagactaa actgaatgtt ctcggacggg actgatggaa cggcgagagc
     3601 cggttttcca tgccggccag tgatgaactg aatctctcct gcttttatgt gcgtatagcg
     3661 ggccagcagc tgccgcaata ctccatgcga gagcacgaag cgttgacgat cccgctcaaa
     3721 cgcaaagcgg gccgcacgcg ttcgttcctg ttcggaaagc tgcgctgcga aggattcctg
     3781 aacagcggca tagcggggca attcgcaaca ccagacatgc acagacgtga gatcgagatg
     3841 gggaaacgaa agggatgcac agatgggatc gggcgcacgc gacatcattt gcctactctc
     3901 acggtcacgg cctgcgttac gctgatgacc gtgccaccgg tttcaggagc gtcggaggac
     3961 gctgcgtagc attgaaggca gcgccggttg acacatcgac gattctcccg tcgtccagtt
     4021 ttaacacgcg gtcgcccaca tggaaatacc ggtcatcgtg cgtcacgaca atgacccctt
     4081 tccctcgggc ccgtagattc ggcaacaatt ctccgtagaa gacgtcctta tattgcggat
     4141 cttgatcggc agcccattca tcaaagacgt aaaacggccg atcttccagc atggcagtca
     4201 ccagggcgag acgttttcgt tgcccctgag acaagttcac cgtcgaatat tcgccctgtt
     4261 caatacggac cttgtgatcg atacgcaggg tcttcagcca tccgtccgcc tgactggcga
     4321 ctaacgacgg gtcgagtccg agcagctttt taaagaggta aaaatcagaa aagaccgcgg
     4381 caaaatgttg gcggtaccag tcctgagtcg cggacgtgat cgcctccccg ttcaatgtca
     4441 cctgcccctg ttgcggcaga tagagtcccg tgagcacttt cacaaatgtc gatttgccgc
     4501 tgccgttccc gccgatgatg aaaaccagct cacccgcgcg aatggaaaga tccagcgacc
     4561 ccaaacgaaa tgaccgttca tcctcgccct tcgattcgta ggcaaacacc gcgttggaga
     4621 attccacaca gggcctgccc tgcgccatcg gacgctccgc tccaccttcc cgaccgcctt
     4681 catccagcgc caaccccaac gattcaatct tttccagcgc cacctgcccg cggctcaacg
     4741 tcggcaccat gcccagcagg ccccacatcg gaccgatcat gtagagcatg gcgaaggcat
     4801 acccggtcag cgattcaccg gacagcgaaa ggacgcgagg aaagagcaag aggatgacgc
     4861 cgatcaaacc gtagaacagc acctgggtcc aggaatccgt gacgagatac tgcttggtcg
     4921 tggtgagatt atgacgccgc agcgcgtcgg ctgcttgccg gatatcctct tcgacaaacg
     4981 tttcccggcg gccgcgatgg agcatcagtt ccttcatgcc ttccgtcaaa ctccggaaat
     5041 gttcgaagag gacgcctttt gaatcacgta ccgccaacga cgattgcagg acacgattgt
     5101 agagctggcg atacccgagc aatcccagta cggccagaat caccacgcct aggaacgcct
     5161 gccaggataa ccaggccaga tagagcgagc agccgcccag gatcgccaca ttgatcgcca
     5221 agcccggcaa cccgttcacc gcccaggcca gggcattcgt gtcgtccgtc agggttgcaa
     5281 gaatctcgtg cgacccgcga cgctccagcg tgcgataggg cgcccgcacc actttccagc
     5341 agagatccat gcccaatttt aagatggtct tttgcgcgaa ggtgaccagc aagagctggg
     5401 acagataatt cgatccgact ttgaggacgg ccaacccgat aaatgccagg gcaaacaact
     5461 ccgaggtggt gccggccccg ttgatcagct tgttgatcac ggccaacaaa cccacgctgg
     5521 agagaccggc caacagcccc gtcatgacca tgaggatcat catggtcctg gcgtcccgaa
     5581 gcagaaacag gagaaaccgg tacaacttca tcatcgtatc gtggcttccg cgtaggggtg
     5641 actcatggct ggcaatcgtg ggagaagcta tgcggccttg gtggttggtc catcaccctg
     5701 accgtcaggc cgcttccggt acacggcttc cgcctcctgc caaaacgcct tgagctgacg
     5761 tgccaattct tgcacatgcg gcgcgacgaa caagcgtccg gaatcttccg ccggaagata
     5821 caccgtccgg ctcatgccca tcgcccattc gccgaacacc aaccgggtat catcactcga
     5881 gttcgtgagg gggtgcttgg atgcgatcac attcaacacc tgcccacgga agggcttcag
     5941 ctcgtaccgc gccacggcat ggaaggtcgc gtatgtgact tgatctttgt ataagaactc
     6001 gtcctgatgc tcggcggctt cggtatggtg catgagattc cacatgcgtt tcaactttcc
     6061 cttccagaac gacgcccatt catcggcagg caaccggcgc atcagccgaa ggtaggtcgt
     6121 gaccttcatc cacacgaaca acaacggcca gagcagatag ggcggacgac tccagtggtt
     6181 caggtaggat cgtggatgcc aggattccat gacagccagg atgacttctt ctccctgtgc
     6241 cgtgaactgt tgtgcgactt cataggcggc caggccgccg gtgcaggtac ctccgatgaa
     6301 atagggaccg tgcggctgca ccgaccggat ctcctcaatg taatgggccg ccatctcttc
     6361 cacccgcatg aacggctttt ctttgccgtc gaggccgcga gcctgcaatc cgtagaaagg
     6421 ttgatcgtct cccaataatt tggcgagccg cgcgaacacc agtacattac ctccaacccc
     6481 gggcacggca aatagtggtg gccgctttcc ttccggctga atggccacga gggagcgcca
     6541 ccgaaccgtg caaccttcgt cggccaggac atccgccagt tgttcgatcg tcggcgcctg
     6601 gaacaaaacg gccatcggta accgcgtccc gaatgtctgc tcaatcgcgc tgaacatgcg
     6661 aagcgccagc agcgaatagc cgcccaatgt gaaaaaattg tcgcgcacac cgatgggcgt
     6721 aatccccaga acctgctccc aaatcgccac cagctgcagc tcgacccgat ttcggggttc
     6781 gatcgcttgg gcgccgtcat gcaccggctc ggcagcgggc gccggcaagg acgcgcgatc
     6841 caccttccca ttgggcgtta acggaaattc cgtgagggga acaatggcag ccggcaccat
     6901 gtaatcaggc agcacctctc gcagtgcccg gcgaaggctg gatgggtcac aggcactacc
     6961 ctctcgcggc gccacatagg ccacaagacg tttatcgccc ggcgaatctt cccgcacgat
     7021 cacgaccgcc tgtttcacgg tcgcatcatt ccccaacact gattcaattt cacccagctc
     7081 gatccggaac ccgcgcaatt tcacctggtg gtcgattcga ccgacataat ccagtcgtcc
     7141 atcgggcaac catttcacct gatcgccggt gcgatagagt cgctcaccag ccttgaacgg
     7201 gttggtgata aaacgctccg ccgtcaattg agactggcct cggtagcctc tggcgagacc
     7261 catcccgccg atatacaact cacctggaat cccgacgggg accggttcga gattgccatc
     7321 caatacgtag acctgcgtgt tcgcgatcgg acggccgaga gaaatcgtcc gttcagcgtt
     7381 acgcacgcgt tccaatgttg accaaatcgt cgtttcggtc ggcccataga cgttccatac
     7441 ggatccggcc ctggcgagca gttcctgcgc caattcgcgc ggcagtgcct ctcctccaca
     7501 caagactttc actccgcgtc cacccttcca cccgaactgc aaaatcattc gccaggtggc
     7561 aggggtcgct tgcagcatgg tcaccgcgcc atgatcgagc tcccgttgca accaggcccc
     7621 gtccatcgcc tgctgccgat tggccagtat gattgtggcg cccaccagga gcggaaggta
     7681 gagttccagc cccgcgatat cgaacgacag cggggtcagt gcgaccagga catcgcgatg
     7741 agtgatcccc ggttcctgct gcatggaacg gagaaaattc accagtgcgc gatgctcaat
     7801 ttcgaccccc ttcggctggc cggtggaccc ggaggtgaac aacacgtaca cgagatgctc
     7861 cggaccggat cgcggcgacg gattctcctc cgcttgacgc gccaggacct ccgcctcctg
     7921 atccagacac agcacaggaa gctgatgctg cggcagcccg ccgagcaagg cggactcagt
     7981 gatcaggact gccgcgtgac tggcctcgat catcgccgcc agccttcgta tcggcaaccc
     8041 cggaaccaaa ggcaggtagg caccccctgc tttcatcacg gccaggagac tgatcagcag
     8101 atccggcgag cgctccagga aaacggggac gaccgcatcc ggccctacgc cacactcacg
     8161 caaacgatgc gccaaccggt tggcgcgacg attcagttcc gcataggacc attcccgccc
     8221 ttcaaagcga acggccacgg catccggcgt ccggctgacc tgcgcctcga acagttgata
     8281 aaaacacagg ccctgatcta ccggtcgatc cgtggcattc cattcgcgca agattcgatg
     8341 ctgctcctgc ccggtgagaa tcgacacgcg cccgagttct gcatcggcat gctcggcaac
     8401 cgtttccatc agctgccggt attgcgaaat ccaccgctcg gcggaggcgc gatcgaacag
     8461 atccgtattg aattctaaat aggcttttcg cgacgcgtag ggatcgatag aaaggccgag
     8521 atcaagttga gccgcgccac ggctcacttc atagggagtc caggagagtt gatgaaactc
     8581 ggtgcgggca aagggcgtat tggcaaagtt gaacaacact tggacgagcg gcaagcctcc
     8641 ctgactacgg tccggccgca attcttccac gagcttctca aaaggaatgt cctggtgcgc
     8701 ataggcgtcg agcgacaggt cccgcacctt tcgcagcagg tcacgaaacg tcagctcacc
     8761 tgtcacgtcc gctcgcaaca ccagcgtatt gacgaaggtt ccgatcagct cctcgatctc
     8821 gagacggtta cggttagcga tcggggtgcc aatgaccagg tccttctgtt gtgtgaggcg
     8881 gtggaggagc ccgaagaatc cggccaggaa taccatgtac agggtaacgc cttcgcgcac
     8941 actcagctgc ttgagacgat tgatcagcga ccaggacaag tcgagcgaca catgatcgcc
     9001 tttgaaggaa tgcaccggca agcgcggccg gtctgtcggc aaggtcagga caggcagatc
     9061 gacaagtttg gtcttccaat gcgcgagttg ttcttccagc acaggaccgg tgagccatcc
     9121 ccgttgccac tgcgcgaagt ccgcatactg aatagcgaga tccggttgaa cggcaaacgg
     9181 tctgcccgcg cagaaggcat tgtaggacgt gacgagttcc cgcgcgatga ttccataaga
     9241 ccattgatcg gagatgatgt gatgcgtatt gaggatcacg atctgatctt catcaccaag
     9301 ctggatcaga aacacgcgca gaagcggtcc ctgttcgaga tcaaacggtc gtcgcgcctc
     9361 cgcggtcgca agttccagag cccgtggttc gcgcagctca gccggcactc cgcgcagatc
     9421 ctcctccgtc caagtcgggt ccaatacgtc ctgaatgatt tgacgtggct gaccgtccac
     9481 gtgcgcaaag gtggtcctga gcgcgtcatg gcgccggacc agttcagcaa ccgcccatcg
     9541 caaggccggc ttgttgagcg gaccatgcaa ccggacgctg gccgggatgt tataggccac
     9601 ggcatccggc gagagtcgat agaggaacca catccgctcc tgcgcgaacg acagcggaag
     9661 cgggattcct cttctggctc gcgtgatcac gtacccactc tccccctgat gggaattggc
     9721 tccggcctgt gcctgtgtga tgacctcggc caaggtggca atcgtcggac gttcgaaaat
     9781 ctgccggagc gggagcgtga tgcggaacag agattggact ctggatacca gttgtgtggc
     9841 gaggagcgaa tgcccaccca gttcaaagaa gtgatcatgc acgccgacgt ctttgagctg
     9901 aagaatctcc ccccagatat cggccaggac ctgttccgtg ggagtacgtg gcgccaggta
     9961 ctgagaggtc agctcgacct tgctggcgcg actgtccgcg gcaacttgca gcgcctttcg
    10021 atccaccttg ccgttcaccg tcagcggaag cgtttccatc tcgatgaagg tcgagggaat
    10081 catgtgcgct ggcaattggc tgcgaagctg ttcgcgcaac tgcaccaatc caagccggtt
    10141 gttcggacgt ggcaccatga acgccacgat ccgcttgtct cctggctggt cctcccgtac
    10201 ctcgaccacc gcccgttcca gatcctgatg ccggttcagg acggcctcga tttcttccaa
    10261 ctcgatgcga tagccgcgaa tcttgacctg gcgatcggct cggccccgat aatccaattg
    10321 cccgtcgggt cgccatcgca ccaggtcgcc tgtacgatac agtcgggctc ccggctgctt
    10381 ggaaaatgga tcgggaacga attgagtctc cgtaagatcc ggcctgcccc gatagccgcg
    10441 cgcgactccg acgccaccga tgtagagctc ccccgccaca cccactggaa ccggctgttg
    10501 attccgatcc aatacgaaga cggacgtatg cggaatgggg cgaccgatgg gcagatcgcc
    10561 gatcaattca tcatgcgcct ccagaccagt cagatccgac caagtgaccg caacggtcgt
    10621 ttctgttggc ccataggtat tgatcaatcg gacggtcgcc ccgatcaagt ccgtccagcg
    10681 ctgcacgatc tgcggcagca ctcgctcgcc accgatgatc accgatctga tagaggaagg
    10741 aaacgcgacc tgctcgagcg ccatccgggt aaccagctca tgccagtagg ccgtgggcag
    10801 atccaggatc gtcacggtta attcacggca gcgatccaga aatccggata ccgaatcgac
    10861 catcgtcgcg gtacgcagca ctaatgtcgc ccccgcggcc agacagggga aaatctcttc
    10921 ggcggcagta tcgaaactca acgaggcaaa ttgtagtact cgatcttctc ctgtcaggtc
    10981 gacgatcttc gtgatggctc gcacatagtt ggcgatcgag cgatgctcga tcatcacccc
    11041 tttcggatga cccgtggacc ccgacgtgta gatcacatag gccagattgt ccaacgcatc
    11101 ggtcacgtcg atagggctgt cgggacactt cgcaatctgc gcccattccg aatcgagaca
    11161 gatcgtatgc ggattcgtgg cgggcagccg cacgagttga tcctgttgtg tcaccaacac
    11221 ccgcacctga ctgtcccgca acatgtattc cagccgttcg gtcggataat cggcatccaa
    11281 cgggagataa gcgccgcccg acttcagaat accgagcagc cccacaatca tgttgagcga
    11341 gcgctcgaga cagaggccaa cgacgacacc gggttcnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnncgca acaccaacat gttcacaaag tcaccaaccg tccgggcgaa
    11521 tcgagcccgg ctacggccga acaccggcgt gacaatcgcc gtgtcttctt gagcggtgta
    11581 gcgagtgagc aggatctgca gcgcgctcag gcagacggca tacaaggtcg tgccctcggt
    11641 ccgcgcaaac ttcttgaggc ggcgagtcag gtcctcgtcg atgtggaagg catgccaggc
    11701 atagtggctc ggctcgaccg ttatctcagg ccggtcgtac agcatgtcat aattcggcaa
    11761 ctcaccggcc agcttcgctt tccagtattg gcccaggcgg ctcccctctt ccccttccag
    11821 gatcgcccga tgccactcga cgaattcccg gtatggtgcg ggacggtctt gaggttccgg
    11881 cgaatactcg gatgaacatg acccggcgac ttcacagcgc cgcttcaaat catcgaccaa
    11941 ctgcatcatt gaccagccat ccacaacgat atggtgggcg accaacaaca accagctctg
    12001 ctgtccgcca tgaaaaagag tcgcgcgcca cagggggccc tgcgtcaggt tgaaggggac
    12061 ggctgcagcc tttacactct cgccccgcaa ctgcggccac tcccaagtgg agctgtcgat
    12121 ttcactccaa ctcaacggca tccggtcatg gattcgttga accggtccat cttcctgcgt
    12181 ttcgaaggtg gtgcgcagca tggcctgctg gtcgccgatg gcttcaaggg ccgctcgcaa
    12241 ccggtctgcc ttaaggtcgg acggcagagg caacaacacc gacacattcg ccgcggcact
    12301 gtcgggatcc agttgactca aaaaccatag ggcggcctgg ttttccgaga gtggaaacac
    12361 cgtcgcgata gcgggatctg caccagcagc aacggactcg gctcgcgatt ccccggccag
    12421 cccccgttca atcatcgtcg tcagatcggc gggtgtcgca ccgcccagaa gcgcggacag
    12481 cgacagcgac agatgaaacg attcttccag gcgatgcagc acatctgctg ccatcaacga
    12541 atcgagtccc aggagatgaa tcggccgatc aaccgcaatg gcctgcggca tgcaaccgag
    12601 ccgatcagcc aatagcttct gcactgtttg cagaatggcg cttgtgcgcg cctcccctga
    12661 aagccgctgc cacgcgtccg tgtgaatggg tgcgggagat gattgtgcca ccggatgcgg
    12721 cgccacactg gttccgatga tcgacaatcg acctgccagg tactgctcac ggcaggcgcg
    12781 ccgttgcacc ttcccactgg aagtcttcgg aaggctgccg gccttgatga agacgatggt
    12841 cgacgcatgg aggtcgtgct gctcggacag ggctgcacgc atggccgcgg ccaagtcctc
    12901 ggtcgaacaa ggcgacgcct ggcgctccac ctcctggacc agcaccacgg tctcttcgcc
    12961 ggcctcctca acagtaaacg ctgccgcgcc tcccgcccgg agcgcgccat ggcatgactc
    13021 gactgttcgc tcgagatcct ggggataatg gttgcggcct cgaacgatca gcagatcttt
    13081 gaaccgcccc gtcacgaaga cttcgccatc cttcacaaat ccgaggtcgc cggtacggag
    13141 gaacggcccg tctccggtat ccgccaggct ggcgccgaac gtgtgtgacg tttcgtcagg
    13201 cttgttccaa tagccttgtg tcgtgctggc gccggacagc cagatttcac cgatctgttg
    13261 caccgcgcaa cggcaccgcg tctcaggatg ggcaatcacc acgcgagtgt cgccgaccgg
    13321 catgccgcat cccacgacct ttcgaactgg cttgtcgtcc gccacagctt cgatgacggc
    13381 acccttttcc aacgctgcgg catccagcgt ggtcacggta ggcgccgctc ctcttcgctt
    13441 gagcgaaatc agcaacgtgt attccgccat tccgtaggcg ggaaagaagg cttcgcgctg
    13501 gaatccggat ggcgcaaacg ctgcggcaaa ccgctggatc gtgtccggtg cgataggctc
    13561 cgccccgcaa ctcgccacct gccactggct aagatccaat cctgcccgtt cctctggcgt
    13621 tgtggcctcc acgcaacggc gataggcaaa gtttggcgcg ccgctatgcg tgaccgcata
    13681 gcgttgaata gcttcgagcc atcgcaaggg gcgtttgagg aacgtgagcg gcgacatgag
    13741 atacgacgga cgcccgatcc acgcaggttg aatgatgccc ttcaccaatc cgtaatcatg
    13801 aaaatggggc atccaggaca gagtcacgga tccggcatcg taaccacccg catcggtgat
    13861 acaccggctc tgcgcggtca tattcccatg gctgaccatc acccctttcg gtgccgaggt
    13921 cgagccggac gtatattgca aatatgcgag gtgtgtgggc agttgctgta ggtcagtcca
    13981 accggaagaa tctgctgaga gcgcctggtc gctcgaaacc cactggtgta acgacgcgac
    14041 aagctcctgc gacctgagtg tctccagcat ctgagctgtg gtcaacgctc ccgcggcgcc
    14101 ggcatcttca gcgaggcgtt gaacgcgcgc cacgccggcc ttcaggcgga acgcatccgg
    14161 aggaggaacc ggcacagcga tgaggccggc atagagaacg ccccaaaagg cttgtacgaa
    14221 ttcaagcccc ggcggatata ccaggaggag tcggtcccct ggcgcgaatc gggcctgaag
    14281 gtagctggcg atcgaacgag cgcgcgtcag caggtctcga tacgtcagta cgctgtcagc
    14341 gctcagtcct tcacggagat agacgtaggc cgtctggtca ggctgatgct cagcccgcca
    14401 acgcagcaca tcgagaaggg tgtcgccggc tggaatggtg accggtggat gtctgaccac
    14461 agaatgctca accccagtgg g
//
LOCUS       DAOQEW010000089        14460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1177_length_14460_cov_9.288511, whole genome shotgun sequence.
ACCESSION   DAOQEW010000089 DAOQEW010000000
VERSION     DAOQEW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14460
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1177_length_14460_cov_9.288511"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1751
                     /gene="ettA"
                     /locus_tag="PLS35_10730"
     CDS             69..1751
                     /gene="ettA"
                     /locus_tag="PLS35_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNI19184.1"
                     /translation="MATNDKQVIFSLVGVGKVYPPKKQVLRDIYLGFYYGAKIGVLGL
                     NGSGKSSLLKIIAGVDPNYVGEITRSKGYSVGLLEQEPQLDPNKTVKEVVEEGKKELV
                     ALLHEYEAVSNSMGDASPDEMEKLIDKQAQLQEKIEAANGWELESELEIAMDALRCPP
                     ADQKVSVLSGGEKRRVALCRLMIQEPDILLLDEPTNHLDAESVQWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGHGIPFQGNYTSWLEQKQERLEKEEKAESKRKKTLEH
                     ELEWIRMSPKARQSKGKARLNRYEELVNQKQDQLAAELEIYIPPGPRLGDVVVEAKGI
                     SKAFGDNVLYENVEFSLPKGGIVGVIGPNGAGKTTMFRMIIGKEKPDSGTIRIGETVK
                     LGYVDQDRSLDPNKTVYEIISDGQDTVMLGKAEVNARGYCARFNFAGTDQQKKVKDLS
                     GGERNRVHLARMLKEGANLIILDEPTNDLDVNTLRALEEGLEGFAGCAVISSHDRWFL
                     DRIATHIMAFEGDSKVVWYEGNYSEYEADRKRRLGKEADQPHRIRYRKLTRN"
     gene            2220..3476
                     /locus_tag="PLS35_10735"
     CDS             2220..3476
                     /locus_tag="PLS35_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNI19185.1"
                     /translation="MSMIWGRTLSRWLAVLALASASCTTIDAPRALPPDHRPAPTRAT
                     LQLGQKELADGRFVGLAFSGGGSRAAVFGAAVMKELERAGLLSQVDVLSAVSGGALPA
                     ASYALEGFGDFRFQDGFVEQIGRDIQGAVAGPWYAAPHNLVRYAFKDTIPAEPVIRAL
                     DDQLFRGATFADLNPSRPLLLLNSTDALTGDPLVIAEERFAPLGIPLAPFSIARAVYM
                     SAAYPGVLEPLKLTHGSTATNGASADPIIAYDGGAADNLGIRTLMQVVERALVNRPMT
                     DLFPQGCLVISIDATGRQRNETHTPLSAAAALLRGHRRNVLELAGIPASRQDVAQFGT
                     FRVGENGGGGSCRFWHVALRQLPDTDPLGDHVTHIKTNLGLSTEDQAALITAAARLVA
                     KGREELNGMAGWAGFLETKPALFSHP"
     gene            3473..4147
                     /locus_tag="PLS35_10740"
     CDS             3473..4147
                     /locus_tag="PLS35_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNI19186.1"
                     /translation="MTRPTDKHRIFITGATGYVGARLIPLLLERGHDVTALVRESSAG
                     KPVVGCRVVVGNPLDAESFAESVRGHDTLVQLVGVPKPSPWKGPQFRAIDGPSAMAAI
                     HAAASTGVQHYVYVSVAHPAPIMRDYIAVRRDCEEALRRAGLVATILRPWYILGPGHW
                     WPLALLPVYRMLERVPATREAAMRLGLVTIREMVAALLWSVEQPPVKTRVIDVPEIRR
                     LGRSEP"
     gene            4144..5022
                     /locus_tag="PLS35_10745"
     CDS             4144..5022
                     /locus_tag="PLS35_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI19187.1"
                     /translation="MKRIALITGAAGLIGGYLVRSASRWVPDWEVVGVTRAEADFTDR
                     AQVEQLWNRHRPALVVHCAALSRIGLCEQDPVRARLTNVEATRLLADLARDVPFIFLS
                     TDQVFDGAKGQYVETDAVHPLNVYAQTKAEAEQVVLANPAHTVVRIALTAGMSPTRDR
                     SFVEDMVRTASKGVTLTLFTDEFRCPIPAGALVRALWELGMQRRPGLYHLGGHERLSR
                     WEIGELVAMRFPELRSSIQPGSVADYQGPPRPPDLSMCSEKIQALLSFPLPGFRQWLT
                     EHPSESGDPWDYPAAR"
     gene            complement(5035..5388)
                     /locus_tag="PLS35_10750"
     CDS             complement(5035..5388)
                     /locus_tag="PLS35_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI19188.1"
                     /translation="MKGEHPVTMAIVMAVGTVLMQADVHGEAAGKADALAKGKQVFTR
                     HCAGCHGAEGKGDGYKLLGPDPANLTAPATRKQSDKALLTTIHEGKPNMPSWKGLLSE
                     RDITSVLAYIRSLPH"
     gene            5566..6366
                     /locus_tag="PLS35_10755"
     CDS             5566..6366
                     /locus_tag="PLS35_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNI19189.1"
                     /translation="MTATDPLSLLMTAWAASALLMVALWLLERRLHNLALADVGWCYG
                     LALVVLWYTTAVPGEPARRLLVCGMIVLYAARLGTHVLIDRLWDKSEDGRYRALRVQW
                     GEQGPLRRFWYFQLQAAAIVWFSLPPLIVMQNPHPPFHLMELLGVLLWMAAVTGEAMA
                     DWQLASFRRQPWNRDRVCREGLWYYSRHPNYFFEWLHWWSYVVMGLASPLGNWALTLI
                     GPLTMGWALLKVTGIPWTEAQTMTSRGEEYATYRRTTNAFFPWFPRRL"
     gene            complement(6607..6828)
                     /locus_tag="PLS35_10760"
     CDS             complement(6607..6828)
                     /locus_tag="PLS35_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19190.1"
                     /translation="MNTFLTWVILILLVSTSACGPDQASHMLETAQFEERQTNKAHAK
                     ELYEDIIRHYPDSPAAKTARTRLTELAKP"
     gene            complement(6926..7237)
                     /locus_tag="PLS35_10765"
     CDS             complement(6926..7237)
                     /locus_tag="PLS35_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19191.1"
                     /translation="MITPPQTPRQRIMTLLTGTLLSSHQLAQLVGMPERQVEDHLTHI
                     VKTLARDTSRTFLLEPSHCQDCTYTFRDRTRLTRPSRCPRCRSEAITSPRYGIREEAG
                     S"
     gene            7352..8815
                     /locus_tag="PLS35_10770"
     CDS             7352..8815
                     /locus_tag="PLS35_10770"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNI19192.1"
                     /translation="MSGSQWDELAELALSRVSSAGVEYADIRLLDTTTRTISGEDRRI
                     AHIRESTDRGFGIRVLHRGAWGFAASSIISLEEIPRVADLAVEIAKGSASLAITKVHL
                     APEPVHRDRIVTPSRLDPFAVPLEEQTALLLSTMDIIQRHPGVVRSHASLWAQRDRKL
                     FVSTEGSHLEFDLLAMQGDCTATAVHNGRFASRSFNTPHLRTGYELVRDADWVREGAR
                     IAEQAVEKVRAPAIDAGRYDLVLDPEHLSLTMHESCGHPSELDRALGYEANYAGTSFL
                     TPDKRGTYRYGSADVNLVADNTEPGTLAATGYDDDGVSCQKWDIVREGIFVGYCTNRE
                     VAPRIKEERSRGSNRADSWGSVPMVRIANIGLEAGTSTLDDLLADVKRGIYIEGHGSY
                     SIDQRRYNFQFGGDAFWLIENGRRTHMVRDVIYHGITPEFWGRCDGVADATHRRRYGF
                     ITCGKGQPGQSGWMTHAASHARFRRVDVISGHTKADA"
     gene            8812..10158
                     /locus_tag="PLS35_10775"
     CDS             8812..10158
                     /locus_tag="PLS35_10775"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNI19193.1"
                     /translation="MTSSITSSWPKLTSRQEFEFLADLVMSHSTGDHTFFSLRDSHGG
                     TTRFANNQIIQNVNLRRGHLSVTVAFGRQHGTASTTDFTAGAVREALKQAESLARLSP
                     DDPEYLPPVGAQTYLSLPTSRPETTAAGPARRLDYAREAIGQCKMENLNAAGTVSSST
                     TTVGLAAETGLRAYEERTEGRFSLTVQAGDATGWSAAAHRSIDRLHVQERTLAAIIKA
                     KRGADEPQELPPGRYTVILEPAAVAGLLSWMIWMLDAKSFYKGTSPFSGKLGSRIIDR
                     RLSLVNQPAHADLLGNSFTHEGLPVIESGWIESGVLTQLLHDRYTAREHQIEPLTTLE
                     SPHLSGERPIGTRVDDLIRTTQRGILVTNFWYIRPVNPSDLTLTGMTRDGTFLIENGE
                     ITSAVRNFRFHESPLRAFNQVETYTAPAEAVTSETGKALVPAMRIHDFHFSSVTKF"
     gene            10311..11018
                     /locus_tag="PLS35_10780"
     CDS             10311..11018
                     /locus_tag="PLS35_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19194.1"
                     /translation="MTRKQQRLAEKPIHLLTLAPDKGEKGAVNPFAPSPLRAAIGKVF
                     VKLEDMTERFEDWCQYGNSEERTEQPLGQRVSNWLGAPVARHIEHQVQAEHGLVPARR
                     WDGAVGDLIFRLRDRAGFVQRALTAQARELKDWVIQHTQSTQAELHDLREQVATQQVQ
                     MEDLSAQLHDLRALVKSQQQVLMYMGKDMEMAPPAELELSLVSPRSIPYRERDKKSSR
                     DRRDTSNEASQIPYLNA"
     gene            11443..13077
                     /locus_tag="PLS35_10785"
     CDS             11443..13077
                     /locus_tag="PLS35_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19195.1"
                     /translation="MKNARYVRGVVYGLVIAGLALPVSGGIARSEVPLFENLGVLQHK
                     ITTHSDLAQKYFDQGLRLVYAFNHEEALAAFTEASRLDPDAPMPYWGVALSLGPNINA
                     GMDSKAESRAVEAVQKATARLAHATPTERAYVEALAARYSVKKAATRKGKDEAYAKAM
                     RRLAHEYPEDADAATIFAEALMVLRPWDYWRADGRPQPGTEEIVTTLEAVLQHQPDHP
                     GACHYYIHAVEASPDPQRGLPCAMRLPSLMPGAGHLVHMPAHIYMRVGRYRDASERNA
                     KAATVDHEYLLQHPLEGNYASGYYAHNLHFLNASLVMEGRSAEALQAARDLLSKISVN
                     EIVKEPSLEWYAPTVLLTMARFGQWGELIRQPPPPKELRYTTGLWHYVRGLAFAATTR
                     FGSAEGELSNLRKSLKGLARAKTAEGKISRTLLKIAERVLAGEIAARKGDFDAGIQAL
                     REALQLEATVPYSEPPFWFQPVRHNLGAVLLLAGRPGDAESVYREDLRLNPENGWALH
                     GLVHSLQAQRKETAQESARLHTAWAQADVTLTGSRF"
     gene            13134..13955
                     /locus_tag="PLS35_10790"
     CDS             13134..13955
                     /locus_tag="PLS35_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI19196.1"
                     /translation="MLKTDYVFQATEEARELQRLQMLERIFDAGTQRRMLATGLTTGW
                     HCLEVGAGAGSIVRWLEQRVGPSGKVVALDTNPRFLRGSSTSTIDILQGDICDTDLPL
                     AMFDLVHARYVMIHIADYQKAFERMLRCVKPGGWVVIEEPDFEAARAVTASDDARTSF
                     TRVTAAIERMFTSLGMDYALGAKLPALFQRQDLSHLTVEHEGHLSAGGGATAQLMKLS
                     AEQLREKYVATGLVTESDIEQYCRLADDPDSWAIYYATVAVTGWWQPQCGGRTQG"
     gene            13958..>14460
                     /locus_tag="PLS35_10795"
     CDS             13958..>14460
                     /locus_tag="PLS35_10795"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19197.1"
                     /translation="MRYIVGVMGPAKARKKDVDNARVLGELIARREWVVLTGGRDVGV
                     MDAACQGAKKVPGSLTIGVLPSARERVSKYVDLAIITEMGNARNNVNVMSSNVVVVCG
                     LLGAGTVSEVALALKAGKPVILVGATPTEEKFFKKLGGRLIASVDSPEEAITLMMKQF
                     EQQQPDLV"
BASE COUNT         2780 a         4180 c         4571 g         2929 t
ORIGIN      
        1 tgccgcgctt tgttatggtc atcgattgca cgatcgaaca gacggttcac actctactga
       61 ggatggctat ggcgacgaac gataagcaag ttattttttc actggtcgga gtgggcaagg
      121 tctatccgcc aaagaaacaa gtcctgcgcg atatctacct gggtttctat tacggcgcca
      181 agatcggcgt cctcggattg aacggttccg gcaaaagctc gctgttgaaa atcatcgccg
      241 gggtggatcc gaattatgtg ggcgagatca cacgctcgaa gggctatagc gtcggtttgc
      301 tcgaacagga gccgcagctc gacccgaaca aaaccgtcaa ggaagtcgtg gaggaaggca
      361 agaaagaact cgtcgccttg ttgcacgaat atgaggcggt cagcaacagc atgggggacg
      421 cgagcccgga tgagatggaa aagctcatcg acaagcaggc gcaactgcaa gagaagatcg
      481 aggcggcgaa tggatgggaa ctcgaaagcg agttggaaat cgccatggac gccctgcgtt
      541 gtccgcctgc cgatcaaaaa gtgagcgtcc tctccggagg cgaaaagcgg cgggtggccc
      601 tgtgccgcct catgatccag gagccggata ttctgttact cgatgagccg accaaccatc
      661 tcgatgccga atcggtgcaa tggttggagc agcatctgca gcaatacaaa ggcaccgtca
      721 tcgcagtgac gcacgatcgg tactttctcg acaacgtggc gggctggatt ctggaattgg
      781 accgcgggca cggcattccg tttcagggca actacacgtc ttggctggag cagaaacagg
      841 agcggctgga gaaggaagag aaggctgaat cgaaacgcaa gaagacgctg gagcacgagt
      901 tggaatggat ccggatgtca ccgaaagccc gtcaatcaaa gggtaaggcg cgcctcaatc
      961 gttatgaaga actggtcaat caaaagcagg atcagctggc ggccgaactg gagatttata
     1021 ttccgccggg accgcgcctg ggcgacgtcg tggtggaggc caaggggatc agcaaggcgt
     1081 tcggcgacaa tgtgttgtat gagaacgtgg aattcagcct gccgaaaggt ggcattgtcg
     1141 gcgtgatcgg cccgaacggc gccggcaaga cgaccatgtt ccgcatgatc atcggcaagg
     1201 aaaaaccgga cagcggcacc attcgcatcg gcgagacggt gaagctgggt tacgtggacc
     1261 aggaccgatc gctggatccc aacaaaaccg tctacgaaat tatttcggac gggcaggaca
     1321 cggtgatgct agggaaggcc gaggtcaatg cccgcggcta ctgcgcccgc ttcaacttcg
     1381 ccgggaccga ccagcagaaa aaagtgaagg acctctcggg tggagagcgc aatcgtgtgc
     1441 acctggctcg catgctgaaa gagggcgcga acctgatcat cctcgacgag ccgaccaacg
     1501 acctcgacgt gaacacgctt cgcgctctgg aagagggatt ggaagggttt gccggttgcg
     1561 ccgtcatcag cagccacgac cgctggttcc tggaccgcat cgccacgcat atcatggcct
     1621 tcgaaggcga cagcaaagtg gtctggtacg agggcaatta tagcgagtac gaagccgatc
     1681 ggaagcgccg gctcggcaaa gaagccgacc agccgcaccg gattcgctac cgcaagctga
     1741 ccaggaacta gcccgcatga ttcatgagca cttctgctgc aatcgccaac tcactgctac
     1801 gacgagcctt cggccagcgg gctcgcccct cggaccctca acgtactgca cgagtacgct
     1861 tcgggtcctc ccgggctccg cgcgccggtc tcgccacgtg actcggcgat ttcgcgacga
     1921 accgtcatga atcatgcggg ctagccggct gttgaaaaag ggctgccggc ggcgttctcg
     1981 cggcgcgaag aggctccacg taccgcaaag ggtacgcatc gcctctacgc tggctgcggc
     2041 cttgccggac agcctttttg aacaacctgc cacggccact gcgcacgtct ctgagtcttt
     2101 gagagtcaca tagttgctgt aggcctgtat ggtgtcagca tcctgttagc gtccgcctca
     2161 acgccaggac tccttactag aagcacatcc attttccgca ttcggccgta gattcatcca
     2221 tgagcatgat atggggacgt actctaagcc gttggttggc ggtgcttgcc ctggcttccg
     2281 cctcctgcac gaccattgat gctccccggg ccctcccgcc tgaccatcgt cctgcaccca
     2341 caagggccac gctgcaattg gggcagaagg aattggcgga tggacggttt gtcggactgg
     2401 cgttctccgg cggaggcagt cgcgcggccg tcttcggggc ggccgtcatg aaggagctgg
     2461 agcgggccgg cctgttatcg caggtcgatg tgctttcggc ggtctccggc ggcgcgctgc
     2521 ccgctgcatc ctatgcattg gaagggttcg gagactttcg gttccaagac gggtttgtgg
     2581 agcagatcgg gcgcgatatt caaggcgcag tggcggggcc ctggtatgcg gctccccaca
     2641 atctggtccg gtacgccttc aaggacacga tccccgccga accggtgatc cgtgctctgg
     2701 acgaccaact gtttcgcggt gccacgttcg cggacctcaa tccctcccga cccctcttgc
     2761 tcttgaactc cacggatgcc ctgaccggcg atcccctggt gattgcggag gaacgatttg
     2821 caccgttggg catccccttg gcgccgttca gcattgcccg ggccgtgtat atgtctgcgg
     2881 cctaccctgg cgtgctggag cccctgaagc ttacccatgg aagtaccgca acgaacggcg
     2941 cgtccgccga tcccatcatc gcctatgatg ggggcgcggc tgacaatttg gggattcgga
     3001 ctctcatgca ggtcgtcgag cgagcgttag tcaaccgtcc catgacggat ctctttccgc
     3061 aaggttgtct cgtcatctcc atcgatgcga cgggccgcca gaggaatgag acgcacacgc
     3121 cgctctccgc cgccgcggcc ctcttgcgag ggcatcgtcg caatgtgttg gagctggccg
     3181 gcattcctgc atcccggcag gatgtggccc agttcggcac ctttcgagtg ggcgagaacg
     3241 gtggcggagg atcctgccgc ttctggcatg tcgcactgcg ccagttgccg gacaccgatc
     3301 cattgggaga ccacgttacg catatcaaaa cgaacctcgg gttatcgact gaggatcagg
     3361 ctgcgttgat taccgcggca gctcgcctcg tcgcgaaggg ccgcgaggag ctgaatggaa
     3421 tggccgggtg ggccggtttc ctggaaacca agccggcgct gttctcgcat ccatgactag
     3481 gccgacggac aagcatcgca ttttcatcac gggcgccacc ggatacgtcg gggcgcggct
     3541 cattccgttg ctgctcgagc gagggcatga cgtgacggcg ttggttcgcg agtcctctgc
     3601 aggaaagcct gtcgtcgggt gccgggttgt ggtcggcaat ccactggatg cggagtcctt
     3661 cgcggaatct gtgcggggac acgatacact cgtgcagctg gtgggcgtac ccaaaccatc
     3721 accctggaag gggccgcagt ttcgcgcgat tgatgggccc tcggccatgg ccgcaattca
     3781 cgccgccgct tcgacgggcg tgcagcacta tgtctacgtc agcgtggccc atccggcgcc
     3841 catcatgcgt gactacatcg ccgtacgtcg tgactgtgaa gaggctctca ggcgagccgg
     3901 attggtcgcc acgattctga ggccctggta cattctcggt cccggacatt ggtggccatt
     3961 ggcgctgttg ccggtctatc gtatgctgga acgagtgccc gcgacgaggg aggccgccat
     4021 gcggttgggt ttggtgacga ttcgagagat ggtcgcggcc ctgctgtggt ctgtcgagca
     4081 gcctccggtg aagaccaggg tcatcgacgt gccggagatc cggcgcctgg ggcggagcga
     4141 accatgaaac ggatcgcact catcaccggt gcagcgggtt tgattggtgg ctacctcgtc
     4201 aggagtgcct cgcgctgggt gcctgattgg gaggtggtag gcgtgacgag ggccgaggcc
     4261 gatttcacgg atcgagccca ggtggaacag ctttggaatc gccaccgccc ggctctcgtc
     4321 gtccattgcg cggccttgag ccgcatcggt ctctgcgaac aggatcccgt acgggccagg
     4381 ttgaccaatg tcgaggccac gcggctcctg gccgacctcg cgcgagacgt gcccttcatc
     4441 tttctttcca ccgaccaagt gtttgatggc gcgaaggggc agtatgtcga aaccgatgcg
     4501 gtccatccgt taaacgtcta cgcacagacg aaggccgagg cggagcaggt cgtcctcgcc
     4561 aatccggccc atacagtcgt gcgtattgcg ctgaccgcgg gaatgtcacc gacgcgcgac
     4621 cggagtttcg tcgaggacat ggtgcgcacg gcatcgaagg gggtgacact cacactgttt
     4681 acagacgagt ttcgctgtcc gatcccggcg ggtgccctgg tgcgggcgct ctgggagctc
     4741 ggcatgcagc gccggccggg tctgtatcat ctgggtggcc atgagcggct ctctaggtgg
     4801 gagatcggag agctggtggc gatgcgattc ccggaattgc ggtcttcgat ccagcccggt
     4861 tcggtggcag attatcaggg gccgccgcgt ccgccggatc tctcgatgtg cagtgagaag
     4921 atccaggcac tgctatcgtt ccctctgccg ggatttcgcc agtggctgac ggagcatccg
     4981 tcggagagtg gcgatccgtg ggattatccc gctgcccgct agattgaggc gaaatcagtg
     5041 cggcagcgaa cggatgtagg ccagcacgct cgtgatgtcg cgctcggaga gaagcccttt
     5101 ccacgaaggc atgttcggtt tgccttcgtg aatggtggtg agcaacgcct tgtctgattg
     5161 cttcctggtc gcgggagcgg tcagattggc cggatccggg ccgagtagtt tgtacccgtc
     5221 gcctttcccc tctgcaccat gacacccggc gcaatgcctc gtgaacacct gtttcccctt
     5281 tgccagggca tcggcctttc ctgccgcttc accgtgcacg tccgcctgca tcaagactgt
     5341 cccgacggcc atgacgatgg ccatcgtaac gggatgttcg cccttcatct ctgttcctcc
     5401 tgtgtatcaa gcacccttgc tctgatatga gcaatcatac gcccgctgcg gtggcaatgc
     5461 catgcgatct cctcctccgt gtgtcgcgat gtccgtcgta gtacaatcat ctgaacacga
     5521 accacacgtg atgtatcttt gcatttcacg tcgatcactg atcgcatgac ggcgacagac
     5581 cccctctccc ttctcatgac cgcttgggcc gcttcagcgc tcttgatggt tgcgctctgg
     5641 ttgctcgagc gccgcctgca caatcttgct ctcgccgatg tgggctggtg ttacgggttg
     5701 gccttggtcg tgctgtggta caccactgct gttcctggcg aaccggcgag gcgtctgttg
     5761 gtgtgcggga tgatcgtgct ctacgccgca cgcctcggca cgcatgtcct catcgatcga
     5821 ctgtgggaca aatcagaaga cggccgctac cgcgccctgc gtgtgcaatg gggcgagcag
     5881 ggtccgctac gccggttctg gtattttcag ttgcaggccg cggccatcgt ctggttctcg
     5941 cttccgccgc tgatcgtcat gcagaatcct catccgccgt ttcatctcat ggagctactc
     6001 ggggtccttc tctggatggc tgccgtgaca ggggaggcca tggccgattg gcagttggca
     6061 tcctttcggc gtcagccatg gaaccgggac cgtgtgtgcc gggaagggct gtggtactac
     6121 tcgcgacacc caaattactt ttttgaatgg ctgcattggt ggagctacgt ggtgatggga
     6181 ctggcgagtc cgctgggcaa ctgggcgttg acgctgattg gcccgctgac gatgggctgg
     6241 gcactcctga aagtcaccgg cattccctgg actgaagcac agaccatgac gagccggggt
     6301 gaggagtatg ccacctatcg ccgcaccacg aatgcgttct ttccctggtt cccccgccgg
     6361 ctgtaacccg gactattcat gaatgcctgc tccggcgtcc gatcctgtcg cccggcgagg
     6421 ctgttctcgc tcggcctcct cgacggactg ccagtacgcc tgcgtcggcc tcacgtgcga
     6481 gcagcctcgg cgggtttcgg tctcgaacac ctcgcgacgg cattcatgaa taatccgggt
     6541 taacaagaag ccaaaaacgt ccgccagcgg cgttcgcgac catcactgca gcgcagctgg
     6601 ctgttgttat ggtttggcga gttccgtcag ccgtgtccgt gcggtcttgg cggcgggact
     6661 gtccggatag tgacggatga tgtcttcgta cagttccttc gcgtgggcct tgttggtttg
     6721 tcgctcttca aactgggcgg tctcgagcat gtgcgacgcc tgatccggcc cgcaggccga
     6781 tgtgctcacg agcaggatga gaatgaccca cgtcaggaat gtattcatgc gacggtcctt
     6841 tcatgtatcg gtcgcgtggc gtgctcattg ccggtctgcc acgctcgcag ggtttccagt
     6901 ggtctggagg agacaggccg catcatcatg aaccggcctc ttcccggatg ccatatcgtg
     6961 gagaggtaat ggcttcgctg cggcagcggg gacaccggct tggtcgcgtg agcctggttc
     7021 gatcgcggaa ggtgtacgta caatcctgac agtgcgacgg ttccaacaga aatgttcgtg
     7081 aagtgtcgcg tgcgagcgtc ttgacgatgt gggtcaggtg atcttccacc tggcgctccg
     7141 gcatgccgac aagttgcgcc aactgatgag aagataacaa cgtaccggtc aacagcgtca
     7201 tgattcgctg ccggggtgtc tgcggtggag tgatcatgcc atcttgattg taggtgaacc
     7261 gcctgtcgat gaaaaggcag gcacgagcga ccgcgatctt gttatactac gcgaaactgc
     7321 ctgtgcattc ggcgcagaga aagacgccgc tatgagtgga tcgcagtggg acgagctggc
     7381 cgaattggcc ttgtctcgtg tgtcttcggc cggagtcgag tatgcggata tccgcctgct
     7441 tgatacgacg acgcggacca tcagcggcga agaccgccgc atcgcccata tccgcgagtc
     7501 caccgatcga gggttcggta tccgcgtgct gcaccgcggc gcctggggtt ttgccgccag
     7561 ctccatcatt tcactcgaag aaattcctcg tgtcgccgat ctggccgtcg aaattgccaa
     7621 aggttcggcc tctctcgcga tcactaaagt ccacctggcc cccgagccgg tgcatcgaga
     7681 ccgcattgtt actccctccc gccttgatcc cttcgcggtt ccattggaag aacaaaccgc
     7741 actgcttctg agtacgatgg acatcatcca gcggcatccc ggtgtggtga ggagtcacgc
     7801 cagcctatgg gctcaacgcg atcgcaaact gttcgtctcc actgaaggtt cgcatctcga
     7861 gtttgacctg ctggcgatgc agggcgattg tacggcaacg gccgtccata acggacggtt
     7921 tgcgagccgg tcgttcaaca ctccccatct gcgtacgggc tatgagcttg tgcgcgacgc
     7981 cgactgggtg cgtgaagggg cgcgcatcgc ggaacaggcc gtggagaaag tgcgggctcc
     8041 ggcaatcgat gcgggacggt acgacctggt gctggatccc gagcacctgt ccttgacgat
     8101 gcatgagtcc tgcggacatc cgagcgaatt ggatcgcgcc ctcgggtatg aagccaatta
     8161 tgccggaacc agttttctga cacccgacaa acgcggcacg taccggtatg gctcagcgga
     8221 cgtgaacctc gtggcggaca acaccgaacc ggggacgctg gcggcgaccg ggtatgacga
     8281 tgacggcgtc agctgccaga agtgggacat tgtccgagag ggcattttcg tcgggtactg
     8341 cacaaatcga gaggtggcac cacgaatcaa ggaagagcgg tcacgcggat cgaaccgcgc
     8401 cgatagctgg ggatcggttc ccatggtccg gattgcgaac atcggtcttg aagcgggaac
     8461 ctccacgttg gatgacctgc tggccgacgt gaagcggggc atttatatcg aaggccatgg
     8521 gtcctatagt atcgaccagc ggcggtataa ttttcagttc ggtggcgacg cgttctggct
     8581 gatcgagaat ggccggcgga cgcacatggt gcgggatgtc atctatcacg gcatcacccc
     8641 ggaattctgg ggccgctgcg acggcgtcgc cgatgccact catcgtcgcc ggtatggctt
     8701 catcacgtgc ggtaaagggc agcccggcca atcgggctgg atgactcacg ctgcatccca
     8761 cgcccgcttc cggcgcgtgg atgtgatcag cggacacacg aaggcggacg catgacgtca
     8821 tcgatcacgt cctcatggcc gaaactgacg agtcgccagg agtttgagtt tctggcggac
     8881 ctcgtgatga gtcactctac gggcgatcac accttcttct cattgcgcga tagccacggc
     8941 ggtaccacgc ggttcgccaa caaccagatc attcagaacg tcaatcttcg gcggggccac
     9001 ctctccgtca ccgtggcctt cgggcgccag catggcaccg ccagcaccac ggattttacc
     9061 gccggggcag ttcgcgaagc cctgaagcag gccgaatcgc tcgcgcggct ctcgcccgac
     9121 gatccggaat accttccgcc ggtcggcgcg caaacctatt tgtccctgcc cacgtcccgt
     9181 ccggaaacga ctgccgccgg cccggcccga cggctcgact atgcgcgcga agcgatcggg
     9241 caatgcaaaa tggaaaatct caacgccgcc ggcaccgtgt cgtccagcac gacgactgtg
     9301 ggattggctg ctgagacggg attgcgcgcc tatgaagaac gcaccgaagg gcggttcagc
     9361 ctgaccgtgc aggccggcga tgccaccgga tggtcggctg cggcccatcg ttccatcgac
     9421 cgcctgcacg tgcaggagcg caccctggca gccattatca aggccaagcg cggggcggac
     9481 gagccacagg agctgccgcc gggccggtac acggtcatcc tggaaccggc tgccgtggcc
     9541 ggactcctca gctggatgat ctggatgttg gatgccaagt ctttttataa ggggaccagc
     9601 ccattcagcg gaaagttggg ctcacgaatc atcgatcgcc ggctctcgct ggtgaatcaa
     9661 ccggcccatg cggacctcct gggaaacagc tttacgcacg aagggttgcc ggtgatcgag
     9721 tccggctgga tcgagtcggg tgtcctgacc caacttctgc atgatcggta tacggctcgc
     9781 gagcatcaga tcgaacccct cacgacattg gagtcgcccc acttatcagg cgaacgtcct
     9841 atcggcacca gggtggacga cctcattcgc acgacgcaac gaggcatcct cgtgacgaac
     9901 ttctggtaca tccgtcccgt caatccgtcc gacctgacgc tgaccggcat gaccagggat
     9961 ggcacattcc tgatagaaaa cggggagatc acgtctgccg tccgaaactt tcgctttcac
    10021 gaaagcccgc tgcgcgcctt taatcaagta gagacctata ccgcgccggc tgaggccgtg
    10081 acgtccgaaa caggaaaagc gctggtgcct gccatgcgca tccacgattt tcacttctcg
    10141 agcgtgacca agttttaggc tggcctgtcg cccagaccct gtctcggtcc gggcgcgtca
    10201 gtcggaccgg gggagaaaaa aacacccact cgccattgac tcggactatc ggaaagagta
    10261 atgaattggg aaaagtttgc cgataaggct cggaaggctt cgatcgtcct atgacgcgga
    10321 aacagcagcg tttggcggaa aaacccattc atcttctcac gctcgccccg gataagggtg
    10381 agaagggggc ggtgaatccg tttgcgcctt ccccattgcg tgcggcaatc ggcaaggtgt
    10441 tcgtcaagct ggaagatatg acggagcggt tcgaagactg gtgccagtac ggcaactccg
    10501 aagagcggac ggaacagccg ttggggcagc gggtgtcgaa ctggctgggc gctcctgtcg
    10561 cgcggcacat cgagcatcag gttcaagcgg aacatgggct ggtgccggcg cgccggtggg
    10621 atggtgccgt tggtgacctc attttccggt tgcgtgatcg tgcggggttt gtccagcgtg
    10681 ccttaaccgc ccaagcccgt gagctcaagg actgggtcat ccagcatact caatccactc
    10741 aggcggaact ccatgattta cgcgagcagg tcgccacgca acaggtgcag atggaagatc
    10801 tgtcggcgca gcttcatgac ttgcgtgccc tggtgaagtc ccagcagcag gtcttgatgt
    10861 acatgggcaa ggacatggag atggccccgc ccgcagaatt ggagctgtcc ctggtgtctc
    10921 cccgatcgat tccctatcgc gaacgggata agaaatcatc gcgtgatcga cgggacacgt
    10981 ccaacgaagc ttcccagatt ccctacctca acgcctagca ggatgcggaa aaagtccgcc
    11041 agcggcgttc tcgcgtcact ccgagactca ccgtaccgcg ggagtacgat tcgcctcttc
    11101 gctcgctgcg gccttgctgg acgatccttt tgcgcatcct gctagatttc tggggtcgag
    11161 caggacgctg aaaacaaccg tcagctttgt tctcacatcg ttcggaccct gaacgtcctc
    11221 cagacggtac gcctccgcgc cttcactcgc tgcggcctcg ctggtccatg attttgatcg
    11281 tcccgcatca atacgtattc agcccttctt tcttctcagc ctgctccgac tagcaacttc
    11341 tcgtcgggac tgctaatttg tgcccttctg tgcgcggcgg agtcgtcgca gtgtgacggc
    11401 attgcccgtc tcgcatacga aaaacgagca ggagcgggga acatgaaaaa cgcgcggtat
    11461 gtgagagggg tggtctacgg cttggtgatc gcaggtctgg cgctgcctgt gtccggtgga
    11521 attgcccggt cagaggtgcc gctgttcgag aatctcggtg tgctgcagca taagatcact
    11581 acccactcgg accttgccca gaagtatttc gaccagggat tgaggctggt ctatgccttc
    11641 aaccatgagg aggctctagc ggccttcacc gaggcgtccc gtctcgatcc tgatgcgccc
    11701 atgccctatt ggggcgtggc gctgagcctg gggccgaata tcaatgcggg gatggattcc
    11761 aaggcagaat cgcgagccgt agaggccgta cagaaagcca cggcacggct ggcgcatgcg
    11821 actcccacag agcgggcgta tgtggaggcc cttgccgcgc ggtattcggt caagaaagct
    11881 gcgactcgca agggcaagga cgaagcctat gccaaggcga tgcgacgcct ggcccatgag
    11941 tatccggagg acgcggatgc agcgaccata tttgctgagg ccttgatggt gctccgcccc
    12001 tgggactatt ggcgagccga tggccgcccg cagcccggga ccgaggaaat tgtgacgacc
    12061 ttggaggccg tgctgcagca tcagccggat caccccggtg cttgtcacta ttacattcat
    12121 gcggtggaag cgtctcccga ccctcaacgc ggcctgcctt gcgccatgcg tctgccgtcg
    12181 ctcatgccgg gcgccgggca tctggtgcac atgccggcgc acatttacat gcgggtgggg
    12241 cgctatcggg acgcctcaga acggaatgcc aaggctgcga ccgtggatca tgagtatttg
    12301 cttcagcacc cccttgaggg caactacgca tcgggatact atgcgcacaa ccttcacttc
    12361 ttgaatgcgt cattagtgat ggaagggcga agcgcggaag ctctgcaggc ggcacgggat
    12421 ctcctgagca agatttcggt caacgaaatc gtgaaagagc cttcgctgga atggtatgcg
    12481 cccacggtcc tgcttacgat ggcccggttt ggtcagtggg gtgagttgat caggcaaccg
    12541 ccgcccccaa aagaactgcg gtatacgacc ggtctctggc attacgtgcg cggtctggcc
    12601 tttgcggcga cgacgcggtt cggcagcgcg gagggtgagc tctcgaatct tcgaaagtca
    12661 ttgaagggat tggccagggc gaagacggct gaaggcaaga tcagccgcac gctgctaaag
    12721 attgccgagc gggtgctggc gggagaaatc gcggcacgga agggggattt cgacgccggg
    12781 atccaggcct tgcgcgaggc cctccagttg gaagccacgg tgccgtatag cgaaccgcca
    12841 ttctggtttc agccggttcg tcacaacctg ggggcggtgc tactcttggc gggacgtcct
    12901 ggcgatgcgg aatctgtgta tcgcgaggat ttgcgcctca atcccgagaa cggctgggct
    12961 ttgcatggat tagtgcatag tctgcaggct cagcggaaag agactgcgca ggagagtgcg
    13021 cggttgcata ccgcctgggc ccaggccgac gtcacactga ccgggtctcg cttctaggat
    13081 cagctgactt gcggccacga ggtggcgtca tggtacggaa taaaggataa gacatgttga
    13141 agacggatta tgtgtttcag gccaccgagg aagctcgtga attgcagcgt ctgcagatgt
    13201 tggagcgtat cttcgatgcc gggacgcaac gccgtatgtt ggcgaccggc ctgacgaccg
    13261 gctggcactg tctggaagtt ggtgcgggtg ccggttccat tgtgcgttgg ttggaacaac
    13321 gtgtcgggcc ttcgggcaaa gtggtcgcgc tcgataccaa tccgcgcttt ttgcgtggca
    13381 gcagcacctc gaccatcgac atcctccaag gagatatctg cgacacggat ctcccgctcg
    13441 ccatgtttga tctggtgcat gcgcggtacg tcatgattca tatcgccgac tatcagaagg
    13501 cgtttgagcg gatgttgcgc tgtgtcaaac cgggcgggtg ggtcgtcatc gaagagccag
    13561 attttgaggc ggctcgcgcc gtcaccgcgt cggatgacgc cagaacctct ttcacgcgcg
    13621 tcaccgctgc aatcgagcgt atgttcacgt cgctcgggat ggactatgcg ctgggggcga
    13681 agttgccggc cttgtttcaa cgacaggatc tgagtcacct aacggtggag catgaaggac
    13741 atctgtcagc gggcggtgga gcgaccgcgc agctgatgaa actctccgcc gaacaattgc
    13801 gggagaaata tgtggccacc ggcctggtaa cggagtcgga catcgagcag tattgccggc
    13861 tcgccgacga tccggattcc tgggccattt attatgcgac agtggccgtg actggctggt
    13921 ggcagccgca atgcggcggg agaacgcagg gataggcatg cgatacattg tcggagtaat
    13981 ggggccggcc aaggccagga aaaaggatgt tgataatgcc cgggtcctgg gtgaactgat
    14041 cgcgcgccgg gaatgggtcg tgttgacggg tggccgcgat gtcggtgtca tggacgcggc
    14101 ctgtcagggc gccaagaaag tccccggtag tttgacgatc ggtgtgttgc cgtcagcgcg
    14161 cgagcgtgtg tcgaagtatg tcgatctggc gattattacg gagatgggca atgcgcgcaa
    14221 taacgtcaac gtcatgtcca gcaacgtggt cgtcgtgtgc gggctgctgg gcgcagggac
    14281 ggtgtcggaa gttgccttgg cgttaaaggc ggggaagccg gtcattctgg tcggggccac
    14341 gccgaccgaa gagaaattct tcaaaaagct gggcggacgc ctgatcgcct cggtggatag
    14401 tccggaagaa gcgatcaccc tgatgatgaa acagtttgag cagcagcagc cggatctggt
//
LOCUS       DAOQEW010000090        14393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1186_length_14393_cov_7.033547, whole genome shotgun sequence.
ACCESSION   DAOQEW010000090 DAOQEW010000000
VERSION     DAOQEW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_14393_cov_7.033547"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLS35_10800"
     CDS             <1..881
                     /locus_tag="PLS35_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HNI19198.1"
                     /translation="NGRGASFQGQQGLSQEDYLAVAQLDGTGDWARILAAAPLRLQDL
                     ERHCAKQIDTVDVLEWDDRSESVRARRQRRFGQLILEDRATHEPDPGQVTAALLVGLR
                     RTGLVALPWTKDLHQWRARVAFLHRVDTTWPDLSDEALTTTLEQWLAPFVTGLMSLAQ
                     LRRIDLQAPLDSLLTWQQRQELSRLAPTHITVPSGSHVRLDYEQGDSPVLAVRLQEMF
                     GCQETPRIAGGTVPVMVHLLSPAGRPVQVTKDLASFWRSAYQDVKKELRGRYPRHHWP
                     DDPFSAQPTNRTKRRT"
     gene            990..1538
                     /locus_tag="PLS35_10805"
     CDS             990..1538
                     /locus_tag="PLS35_10805"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNI19199.1"
                     /translation="MDHFAIVTAFGQDRPGIVALMADSLYQLGCNIEDSCMTRLRGEF
                     TMMLMVRLPQGIDAEALGRRLTVSTRPLDLAVLCRALPDQAALRQPMPEVPTFMLSVY
                     GADHPGIVAQVARTVADHGGNITDMNTRVIGSGEVPVYVMVLEIQLPEDRQSDQLKQA
                     LEALKPLLGVDLTFRPLDSVTF"
     gene            1541..2038
                     /gene="def"
                     /locus_tag="PLS35_10810"
     CDS             1541..2038
                     /gene="def"
                     /locus_tag="PLS35_10810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNI19200.1"
                     /translation="MAIRPILHYPHPTLKSESAPTLPSDPATPVVVQDLLDTLAASPG
                     VALAAPQIGYALRVIVVDVSRKKGEKGHGLIVLINPVILVLEGPKILREGCLSVPDYT
                     GNVVRYEQAVVEGLAPDGRVVTLTTSGFEALAFQHEVDHLNGLLFLDRIQSLSTDLFR
                     RKTQS"
     gene            complement(2069..2518)
                     /locus_tag="PLS35_10815"
     CDS             complement(2069..2518)
                     /locus_tag="PLS35_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI19201.1"
                     /translation="MMMDGRQWAPILATAAILMVAVVSPALADPASEHQLQALAGQGD
                     ANAQWMLGQALLTGNLGTTDEAEAVRWLQLAADQGHVLAQRDMGMLYEQGQGVTQDTL
                     EAFFWYSLASRQDSGRAKLRRDVLAAMLTPDQREAVAARLKGWRPSR"
     gene            complement(2533..3783)
                     /locus_tag="PLS35_10820"
     CDS             complement(2533..3783)
                     /locus_tag="PLS35_10820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNI19202.1"
                     /translation="MSSSARVIIIGAGLAGLACAQHLVRRGLACTVLEASDEAGGRVR
                     TDRVDGFQFDRGFQVLLTGYPEAAKVLDYPSLQLQSFHPGALVRYGGRFHRISDPLRR
                     PQDLVAGLMSPIGSFRDKLTILRLRQDALNQRLCAQAGGGNRTTLEALDRYGFSAVMR
                     ERFLRPFLGGVFLDQSLSTPCRHFELVWAAFSRGAIALPQAGMGAIALQLASSLPAGS
                     IRLRAPVTQLDGKQVVLASGERLSADAVVLATDYATAATLRGEPVPAAPGRRSTTLYF
                     DAPTPPVPGPWLLLNGEQQGLIGTACVLSEVAPAYAPSGRALVSVSLADQTAPVDADQ
                     QQIVRAQLREWFGEAVDEWRHLRTDRISRALPPVDVLASRNGAASPRLRPALYQCGDY
                     CETGTLDGALLSGRHAAAAILADL"
     gene            complement(3793..4188)
                     /locus_tag="PLS35_10825"
     CDS             complement(3793..4188)
                     /locus_tag="PLS35_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19203.1"
                     /translation="MMLKYVGVMLCSMLLWANAASAEWMKVGGTHKYDGYVDMATIGP
                     TGKTVTLWTLKDFKVDRQIPQGTYRSVRIKKQIDCEGHKSRPLHNKYYASQMGKGRPM
                     QSGKATREWSRVTPGSDGEAEFKIACKKV"
     gene            4291..5145
                     /locus_tag="PLS35_10830"
     CDS             4291..5145
                     /locus_tag="PLS35_10830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNI19204.1"
                     /translation="MQPSPQLLFHPPAVYCQAMTRKPSGTRRGEPAAIRPTPPADLDR
                     EKRVTLDRLLSKLGIASRSQAQEWIRAGRVRINQRLVRQPDTWVVWPGDAVTLDEQPL
                     QQGAPRFILFHKPKGVVTTHADEQGRRTIFDVLPPEFTRLHAVGRLDQATSGLLLLTN
                     DTALSSFLTDPKQRIRRRYLVTVRGEVTDDRAQAAIDGLEDQGERLHCAALTIQKRSG
                     RESHLDVTLTEGKNREIRRLFNALGHEVTRLRRIQYGPFALTDLEPGAWREIPIDAAK
                     ALLDKGLP"
     gene            5383..5832
                     /locus_tag="PLS35_10835"
     CDS             5383..5832
                     /locus_tag="PLS35_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19205.1"
                     /translation="MKEIMKRFATGLTTLAFAAALLAPALTYANGADKAPLEIKVRVS
                     EKGFFDEKGKPYGGKRMLQVPKDTMVKITFVFGEDLTSLAAGDTHQITVRAEDGWKQE
                     TSPLWMMNRESTVSFLAGEKGRMQYRAYCILDCIGMEHLTNLFIQVV"
     gene            complement(5944..6360)
                     /locus_tag="PLS35_10840"
     CDS             complement(5944..6360)
                     /locus_tag="PLS35_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19206.1"
                     /translation="MTKYVSRSIIGISLGLLLWSGTGLATEPADVEKFVNARIEIGEM
                     MTNYFKGGESYGSGQRPSPEKMKEMGDDINGKLSDLLSKRGLSLEEYRVRSKEIFADK
                     EAVDAYLAKHPDLKARYDALPLSRMSGGGGSTGRGY"
     gene            complement(6464..7378)
                     /locus_tag="PLS35_10845"
     CDS             complement(6464..7378)
                     /locus_tag="PLS35_10845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI19207.1"
                     /translation="MLVSRILLVLALAGFVGCAGTTPPVRTNSGAHPPQLADVIPPDQ
                     PFQRHMVDLHGLHLSVLEAGTGDPIIFVHGVVTTSNIFPKYVGAYSPEFRGIAVDLRG
                     YGDSEKPATGYSIPQFSNDLIKLADVLQIKKAVWVGVSMGGMILQRLALDHPERVRAL
                     VLVSTTDGPMILDKDIPTIGAPRDYREVSKNMIVESFPADTQAKTYQPLLDRIPTWNG
                     TVIREALTSMSQFNVHGQLTRIQVPTLIMVGVKDDVATPAIAKGIQAQIAGSQVVEYQ
                     TGHFMMAEDPDRFRAVLGEFLKGLPPSQ"
     gene            complement(7493..7801)
                     /locus_tag="PLS35_10850"
     CDS             complement(7493..7801)
                     /locus_tag="PLS35_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19208.1"
                     /translation="MKDVTISPSLVARSFKSDRAKPLSRGLSDLAGWLFTVNADRWRR
                     RAQFYERHRDYFPRVAASPFVDRCRELEAASREFAEVLFAMPPRVDLVPIQVTVSRIK
                     "
     gene            complement(7935..8687)
                     /locus_tag="PLS35_10855"
     CDS             complement(7935..8687)
                     /locus_tag="PLS35_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI19209.1"
                     /translation="MAKITEVAPDLFRITTFLEPFNLQFSQFLVRDEQPLLFHTGPRA
                     LFADVKEAVASLIDVRTLRWISFSHFEADECGSLPDWQGVAPQSDAVCSLVGKLVSVD
                     DCLALRPAKGMADGEVLQTGKYRFQFLATPHVPHCWESGLLFEETQRTLLCSDLFHQA
                     GDGEPVTQSDVVGRCREVLMEYQQGPLANYMPYCTLTDPTLNRLAALKPNTLATMHGS
                     VYAGDGAQALRDLAHVFREILGSPAPVSMPSP"
     gene            complement(8787..9272)
                     /locus_tag="PLS35_10860"
     CDS             complement(8787..9272)
                     /locus_tag="PLS35_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19210.1"
                     /translation="MAWLCYAAPLLFMIGPAAVMAHEPLTSILFHSAIGFSLSALIHR
                     IGHALLTPLPKGPHAIYLSATRRLPLRLTLSQFVWLLEGLVALGLFCGLLSKRCWLAG
                     GTEMLVAGWVLFLLGGGLFFLPVRLVKLWEMRHYPATSLVGPSDDVINRSLSCLPRLF
                     K"
     gene            9682..9861
                     /locus_tag="PLS35_10865"
     CDS             9682..9861
                     /locus_tag="PLS35_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19211.1"
                     /translation="MKQQIQRPVRRTEQQKPTQDKDGVWPLSAREFMKLPVSGLLSTG
                     KRIRFTRLTPPDQAA"
     gene            9890..10078
                     /locus_tag="PLS35_10870"
     CDS             9890..10078
                     /locus_tag="PLS35_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19212.1"
                     /translation="MLIEDSLLTHDERKAAEAAFRGLPLDAQWSRGAQTIYIGILEVT
                     RGRDIVGDDLWEEVAAAV"
     gene            complement(10152..10496)
                     /locus_tag="PLS35_10875"
     CDS             complement(10152..10496)
                     /locus_tag="PLS35_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNI19213.1"
                     /translation="MLNAEVMAEVLEFRRKRDWEQFHKPKELAAAITVEASELLEVFQ
                     WKTHEEVARLLGGPSRERVTDEIADVVILLSYLCHDLGLDVNEAVRAKLKKNEAKYPV
                     EKSYGNARKYDE"
     gene            10715..11263
                     /locus_tag="PLS35_10880"
     CDS             10715..11263
                     /locus_tag="PLS35_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19214.1"
                     /translation="MNRRLLSALSLLLMLTAPVYAQSPCSDCFYAAEEEVRKCLDNAI
                     SAGDRNSCLDNRRVRLKACSDNQCQTVREETAITETQSTVSRPGVAPYTPTEAEWLAL
                     ITRAGLRREATPDRPYSLDIILADPQTLQIMVRHASTMDREQLRKAIEGARETIRNTA
                     KSYGWDKWVKIRESVEMYAAKK"
     gene            complement(11291..11797)
                     /locus_tag="PLS35_10885"
     CDS             complement(11291..11797)
                     /locus_tag="PLS35_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HNI19215.1"
                     /translation="MLSNPSREQSHARTLTSYPLNLQGRIYGSAMPFGLYDPEGTLLP
                     KFRAAAIHGVVLLAEPDECREKTGRDLLAMYREEGLTVLPLPIPNYGIPVDGGLPEVL
                     KRVHEEAVQGHNVVIHCSAGLGRTALFATLLARSVLGLSGPDALAWLGRHQPGALLTP
                     AQIMLIME"
     gene            complement(11787..12872)
                     /locus_tag="PLS35_10890"
     CDS             complement(11787..12872)
                     /locus_tag="PLS35_10890"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HNI19216.1"
                     /translation="MNILQATHEYERWSARQVPFVPADLRLKHRMMTQSAFSFLRATC
                     YRWLQVWPEVCADLTAAPALRAVADLHVENFGTWRDSEGRLIWGINDFDEACVFPYAS
                     DLVRLVVSAKLAGRAEHLAVKLDEACDAVLTGYLEGLRAGGRPFVLSEQHAWLRDIAT
                     NSLRDPVKFWKKMAALPMLKDTVPLEVTNALEKVMPEAGLSYGLRRRVSGLGSLGRPR
                     VVALADWRGGKIAREAKRLIASAGLWARGQKPIGMLLYQLMLEQSVRCRDPFVGMDGS
                     WLLRRLSPYCSRIEVTALPKKRDEGKLLYAMGWETANVHLGDRAQIKAVQRDLAARKS
                     AWLKKAAKAMLKTTVADWKDWAEHHAE"
     gene            complement(13000..13695)
                     /locus_tag="PLS35_10895"
     CDS             complement(13000..13695)
                     /locus_tag="PLS35_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNI19217.1"
                     /translation="MMKTGRASFHVDHAAESRLGFPTVTVLPPKHFSMLREFHLADVL
                     TLGNAACGLAGVFFAMQYMSSHSLAHFLAAAALSPAALLFDWFDGRVARWRHQQSVLG
                     RELDSLADIISFGVAPAALAFAAGLRGGWDWIALTYFVCCGVSRLARYNVTAERLSAG
                     HDKVAYFEGTPIPTTALLTGVLAWAAWQDRLGEQLYGGVWTIGPGDLHALSLLFILSG
                     TLMISKTLHIPKL"
     gene            complement(13810..14127)
                     /locus_tag="PLS35_10900"
     CDS             complement(13810..14127)
                     /locus_tag="PLS35_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19218.1"
                     /translation="MMKRTLQMCLVAALLTGGGGFAFAADPMKDKEASPTLGERITKD
                     AVKGTLMKMDGEFYQIRNTDGELVRVHVDKSTKLDKVVEGDMVKAYVTDKGHVTTLQR
                     IEK"
BASE COUNT         3053 a         4607 c         4132 g         2601 t
ORIGIN      
        1 ccaatggacg cggcgcgtcg ttccaaggcc agcagggtct ctcgcaggaa gactacctcg
       61 ccgtggcgca gctcgacggc accggcgact gggcacgcat ccttgccgcc gcgccgttac
      121 gtctccagga cctcgaacgc cactgcgcaa agcagatcga cactgttgat gtgctggaat
      181 gggatgatcg atcggaatcg gttcgtgcgc gacgccaacg gcgcttcggc caattgattc
      241 tggaggaccg cgccacccat gagcctgatc ctgggcaggt cactgccgca ctgctcgtcg
      301 ggctccgtcg taccggactc gttgccctgc cctggacgaa ggacctacac cagtggcggg
      361 cacgcgtcgc gtttctccat cgcgtcgata ccacctggcc cgacctgtcc gacgaagctc
      421 tcaccacaac ccttgagcaa tggctcgccc cctttgtgac gggactcatg agcctggcgc
      481 aactccgtcg catagacctg caagcccccc tcgacagcct gctgacctgg cagcaacgcc
      541 aggaattgtc ccgcctcgcg ccgacgcaca tcaccgtgcc gagtggctcg catgtccggc
      601 tcgattacga gcagggcgac tcgcctgtgc tggcggtacg cttgcaggaa atgttcggat
      661 gccaggagac acccagaatc gcgggaggaa cggtgcccgt catggtccac ctgctctcac
      721 cggccggcag gcccgtgcaa gtcaccaaag acctggcaag tttctggcga tcggcgtacc
      781 aggatgtgaa gaaggaattg cgcggccggt acccacgcca ccattggcct gacgatccgt
      841 tcagcgccca gccgaccaac aggaccaagc gacgcacgta accattcagc gatgaccgat
      901 tcgaagaaca ccgcctctac ttgccaatcg tcaccgaatc ccgcatgcta cgcggcacgt
      961 ctgacggacg atcaccctac gagttttcca tggaccactt cgccatcgtc accgccttcg
     1021 gtcaggaccg ccccggcatc gtcgccctca tggccgatag cctttaccaa ctcggctgca
     1081 acatcgaaga cagctgcatg accagactca ggggcgagtt cacgatgatg ctcatggtcc
     1141 gcctgccgca aggcattgac gccgaagcct tgggccgtag actcaccgtg tctacacgac
     1201 cgctggacct tgccgtgctc tgcagagcgc ttccggatca ggcggcgctc cgccagccca
     1261 tgccggaggt tccaaccttc atgttgtcgg tgtacggcgc ggaccatccc ggtattgtgg
     1321 cgcaggttgc acgcaccgtg gcggaccacg gcggcaacat caccgacatg aacacccgcg
     1381 tcatcggttc gggcgaggtg cccgtctacg tcatggtgct tgaaatccaa ctgccggagg
     1441 accggcagtc ggatcaactg aaacaggcac tggaagcgct caagccattg ctaggtgtcg
     1501 atctgacgtt ccgcccgctc gacagcgtga cgttctaatc gtggcgatcc gtccgatcct
     1561 gcactatccc catcccacac tgaaatccga gagcgctccc actctgcctt ccgatccggc
     1621 aaccccggtc gttgtgcaag acctgctcga taccctggcc gcatctcccg gagtggccct
     1681 tgccgcgccg cagatcggct atgccctgcg tgtgatcgtc gtagatgtgt cgcgcaagaa
     1741 gggcgagaag ggccatgggc tgatcgtgtt gatcaacccg gtgattctcg tgctggaagg
     1801 gccaaagatt ctgcgggagg gctgcttgag cgtgccggac tacaccggca acgtggtacg
     1861 ctatgaacag gcggtggtgg aaggcctggc gccggacggc agggttgtca cactgaccac
     1921 gtcaggattc gaggcgctgg cgtttcagca tgaagtagac catctgaacg gtctgctgtt
     1981 tctggaccgc attcagtcgc tcagcacgga cctcttccgc cggaaaacgc agagctgaga
     2041 agcatcaagc cctgctcgtg cgagaagatc atcggctggg ccgccacccc ttgaggcgag
     2101 cggccaccgc ttcgcgctgg tctggtgtca gcatggctgc caggacatca cgccgcaatt
     2161 tggcccgccc actgtcctga cggctggcga gggaatacca gaaaaaggct tccagcgtat
     2221 cctgggtcac gccctgcccc tgctcgtaca acatgcccat gtcgcgttgc gccaacacat
     2281 ggccctgatc tgcggccaat tgcagccaac gcacggcctc ggcttcatcg gtcgtgccca
     2341 ggtttcccgt gagcaaggct tggcccaaca tccattgcgc attcgcatcg ccctgcccgg
     2401 cgagcgcttg tagttggtgt tccgaggcgg gatcggccag tgcaggcgac accacggcga
     2461 ccataaggat tgccgccgtc gccagtatag gtgcccactg tctcccgtcc atcatcatca
     2521 ccccatcgtc gctcacagat cggccaggat cgcggcggcg gcatggcgcc cggacaacag
     2581 cgccccgtcc agcgtaccgg tttcgcagta gtcgccgcat tgatacaaag cgggccgcag
     2641 gcgcggagac gcggcgccat tccgactcgc aagcacatcg accggaggca acgcccggct
     2701 gatgcgatcg gttcgcaggt gccgccattc gtctactgcc tcgccaaacc attcacgcaa
     2761 ctgtgctcgt acgatctgct gctggtctgc gtcgacaggc gccgtctgat cagcaaggct
     2821 caccgaaacg agcgcccgtc cagaaggggc ataggccggc gcgacctcgc tcaacacaca
     2881 ggccgtgccg atgagccctt gctgttctcc gttcaacaac aaccaaggcc caggcacagg
     2941 cggagtgggt gcgtcgaaat agagcgtcgt cgaccgacgg ccgggtgcgg ctggtaccgg
     3001 ctctcctcgc aacgtggccg ccgtggcata atcagtcgcc agcacaacgg catcggccga
     3061 gagacgttcg cccgaggcga gcacgacctg cttgccgtcg agttgcgtga caggcgcacg
     3121 taggcggatc gaaccggctg gaagtgagga ggccaactga agcgcgatcg cgcccatgcc
     3181 ggcctgcggc aacgcaatcg cgccgcgcga aaatgccgcc cacacgagtt caaagtgccg
     3241 gcagggcgtg gagagggatt gatccagaaa cacgcccccg aggaacggcc ggagaaaccg
     3301 ctcgcgcatg accgcggaaa atccatagcg gtccaaggcc tctaaggtcg tacgatttcc
     3361 gccgcctgct tgcgcgcaga ggcgctggtt cagcgcgtcc tggcggaggc ggagaatcgt
     3421 gagcttgtca cggaacgagc cgatcggact catcaacccg gccaccagat cctgagggcg
     3481 acggagtgga tcactgatgc gatgaaagcg gccgccgtaa cgtaccagcg cgccgggatg
     3541 aaaggattgc agctgcagac ttggatagtc gagcaccttc gcggcctcgg gatagccggt
     3601 gagcaacacc tgaaagcctc gatcgaattg aaagccatcc acccgatcgg tgcgaacccg
     3661 gccccccgcc tcatccgatg cttcgagaac agtgcaggcc agccctcgtc tgaccaggtg
     3721 ttgtgcgcaa gccagcccgg caagaccggc accgatgatg atgacacgag cgctggaaga
     3781 catgaggcgc cgctacacct ttttacaggc gatcttgaac tccgcctcgc cgtcactccc
     3841 tggcgtgacc cgcgaccatt cgcgtgtggc ctttcccgat tgcatgggac ggcccttccc
     3901 catctggctc gcataatatt tgttgtgtaa cgggcgactc ttgtggccct cgcaatcgat
     3961 ttgttttttg atccgcaccg atcgataggt gccctgcgga atttgtcgat ccaccttgaa
     4021 gtccttcaac gtccagagtg tgaccgtttt gcccgtcggg ccgatcgtcg ccatgtctac
     4081 atacccatcg tacttatgcg tgccgccgac cttcatccac tcagccgacg ccgcattcgc
     4141 ccacagcaac atcgagcaga gcatcacccc cacatatttc agcatcacac ctgactcccc
     4201 ctcgacatat cattcagtcc tgacgatcat gcgccgcaac cgcacaggta cggcaaaacc
     4261 gccgtcatca taccatcgga ggccgatgac atgcagccgt cgccgcagct tctctttcac
     4321 cctcccgcag tgtattgtca ggccatgacc agaaaaccgt cgggcacccg aagaggcgag
     4381 cccgcagcaa ttcgcccaac ccctcctgcc gatctcgacc gcgagaaacg cgtgacgctg
     4441 gaccgtctcc tctcaaaact cggcatcgcc agtcggagcc aggcacagga atggatcagg
     4501 gccggacggg tccgcatcaa tcagcgcctc gtacggcagc cggacacttg ggtagtctgg
     4561 cccggtgatg ccgtcacgct cgacgaacag ccgcttcaac aaggcgcccc gcgattcatc
     4621 ctgtttcaca aacccaaagg agtggtcacc acgcacgctg acgaacaagg ccggaggacc
     4681 atttttgatg tactgccgcc ggagttcacc cgcctgcatg ccgtcggacg gctggaccaa
     4741 gccaccagcg gcctgctcct cctcaccaat gacacggcac tctccagctt cctcaccgac
     4801 ccgaaacagc ggatacgacg gcgttacctg gtcaccgtgc gcggcgaggt cacggacgac
     4861 cgcgcgcagg cggccatcga tggactcgag gatcagggcg aacggcttca ctgcgcggcg
     4921 ctgacgattc aaaagagatc cgggcgtgaa tcgcatctgg acgtgacgct gaccgaaggg
     4981 aagaaccggg aaattcgacg cctgttcaac gcgctgggtc acgaagtcac gaggctgcgc
     5041 cgcattcaat acggcccctt tgcgctcact gatcttgaac cgggcgcctg gcgcgagatc
     5101 ccgatcgatg ccgccaaagc cttgctggac aagggtttac cctgagtcga cacctatctt
     5161 cttttcctac gtgctgcgcc tgctcatccg gtgaaagaac ttgccaacca ccggtgctgt
     5221 gctaaggtca atacagcagg cgaagcgttc gggaacacac agtccgcatc gtactgaaca
     5281 acccactccg ctctcgtctc cgcaacatta caccaccgtc gctcccgacc agggaagggc
     5341 cggctcgaac agcagccggt cggaagatag gagatcagga tcatgaagga gatcatgaag
     5401 cgattcgcca ccggcctcac caccttggcc tttgccgccg ccttgctggc gccggcgctg
     5461 acctatgcca acggcgccga caaggctccg ctggagatca aggtgcgcgt gagcgaaaag
     5521 ggattctttg atgagaaagg caaaccctat ggcggaaagc ggatgctaca ggtgcccaaa
     5581 gatacgatgg tgaaaatcac cttcgtcttc ggtgaagacc tcaccagttt ggccgcggga
     5641 gacacgcacc agattaccgt tcgcgctgaa gacggctgga agcaggaaac cagccctctg
     5701 tggatgatga atcgggaatc gacggtcagt ttcctcgccg gcgagaaggg ccgtatgcag
     5761 taccgcgcct actgcatcct cgactgcatc ggcatggagc acttgacgaa tctcttcatc
     5821 caggtcgtgt aaggcggctt cccgggtcag cgagactcac gaaatgaaaa cgctcggcgt
     5881 actgatgcat acgccgggcg tttgattttt tgagacggtg attcagcggc ggatccccgc
     5941 ctcttagtaa ccccgaccgg tgcttcctcc acccccgctc atccggctca gcggcaacgc
     6001 gtcataacgc gccttcagat cgggatgttt ggccaggtag gcgtcgaccg cttccttatc
     6061 ggcgaagatt tctttgctgc gcacgcgata ttcttccagc gacaggcctc gcttcgacag
     6121 caagtcactg agcttgccgt tgatatcgtc gcccatttct ttcattttct ccggcgacgg
     6181 ccgctgccct gatccgtacg attccccgcc tttgaaatag ttcgtcatca tttcgccgat
     6241 ttcgatgcgg gcattcacaa acttctccac atcagccggt tccgtcgcca ggccggttcc
     6301 cgaccacagc agcagaccga gtgagatgcc gatgatcgat cgcgatacgt acttggtcat
     6361 aatctcccct ctgagtgata gagccatgtg aactgtcttc cggcatcata ccatcccgcc
     6421 ggatgcggcg cacgggccgc gaatcgcccg cgtgcctgcc gcactactgc gatggcggca
     6481 accctttcaa gaactccccg agcacggcgc gaaaccgatc ggggtcttcc gccatcatga
     6541 aatggccggt ctggtactcc acgacctgcg aaccggcgat ctgcgcctgg atgcccttgg
     6601 caatcgccgg tgtcgccaca tcatccttga cgcccaccat gatcaatgtc ggcacctgga
     6661 tgcgcgtcaa ctggccatga acgttgaact gcgacatgga ggtcagtgcc tcccggatca
     6721 ccgtgccgtt ccaggtcgga atacggtcga gcagcggctg ataggtcttc gcctgcgtgt
     6781 cggccgggaa actctccaca atcatgtttt tcgacacctc gcgatagtca cgcggcgcgc
     6841 cgatggtggg aatgtccttg tccagaatca tcggcccgtc ggtcgtggaa accaagacca
     6901 acgcccgcac acgctcagga tgatccaacg ccagccgttg cagaatcatg ccgcccatcg
     6961 acacacccac ccacacggcc ttcttgatct ggagtacgtc ggccagtttg atcaggtcgt
     7021 tggaaaactg aggaatggaa tagccggtcg ccggcttttc cgaatcgcca tagccgcgta
     7081 aatctaccgc gatgccgcga aactccggcg aataggcccc gacatatttg gggaagatat
     7141 tgctggtggt gactacgccg tggacgaaga tgatcggatc gcccgtcccg gcttccaaca
     7201 cgctgagatg aaggccgtga agatcgacca tatggcgttg aaacggctga tcaggcggaa
     7261 tcacgtccgc gagttggggc ggatgtgcgc cgctgttcgt gcggactgga ggggtcgtgc
     7321 cggcgcagcc gacaaacccg gccagggcca gtacgagaag gattctagaa accagcatat
     7381 ggctcccctt tctgaatagg ctgacagcta atggcttagc gtaagctgtc agccgcacag
     7441 attcaatgct ggaatcccat gagaggaggg atgtggttcg aatccccgtc cattacttga
     7501 tccgggagac cgtcacctgg atcggcacca agtcgacccg tggcggcatc gcgaacaaca
     7561 cctcggcgaa ttcgcgagag gccgcctcca gctcccgaca acgatccacg aacggactcg
     7621 cggccacgcg cgggaaatag tcgcgatggc gttcgtaaaa ctgtgcccgc cgccgccagc
     7681 gatcggcatt gacggtaaat aaccagccgg ccaggtccga gagtccccgc gacaacggct
     7741 tcgcccgatc cgacttgaaa ctgcgtgcaa ccaacgatgg agatatggtc acgtctttca
     7801 tggctgcgcc tccctcccat gcgcccgatt cgacgcccgt catcatggcg gccgggtggc
     7861 gtctctgccg gctcaccttg cctcactgta ggccggcgac atgaggaatg gattagttca
     7921 gcatgaaaaa gatttcatgg agatggcatt gagacggggg ccgggctacc gaggatctcg
     7981 cggaagacgt gagccaggtc gcgcagcgct tgcgccccgt cgcccgcata gaccgaacca
     8041 tgcatggtcg ccagagtgtt tggcttcaac gccgccaacc ggttcaaggt gggatccgtc
     8101 aacgtgcagt agggcatgta gttggccagc ggaccttgtt gatattccat gagtacctcg
     8161 cgacagcgcc cgacgacatc ggactgggtc accggttcgc catcgcccgc ctgatgaaac
     8221 aggtcggaac agagcagcgt gcgctgcgtc tcttcgaaca acaggcccga ctcccagcaa
     8281 tgcggcacat gcggcgtcgc cagaaactga aaacggtact tgcccgtctg cagcacctct
     8341 ccatccgcca ttcccttcgc cggacgaagc gccaggcaat catccacgct caccagtttt
     8401 ccaaccaggc tacacacggc gtctgactgc ggggcgaccc cctgccaatc cggcagcgac
     8461 ccacattcgt cggcttcgaa atggctgaag ctgatccagc ggagcgttcg cacgtcgatc
     8521 agggaagcca ccgcctcttt cacatcggca aacagtgcgc gcgggccggt gtgaaacaac
     8581 aacggctgct cgtcacgcac caggaattgg ctgaattgaa ggttaaacgg ttcaaggaag
     8641 gtcgtgatac gaaacaggtc cggagcgact tcagtaatct tcgccatgat gccctccagc
     8701 atgaatgtgg ctggcatcgt aacgggtgga cgggacagga tcaagatgcg cagaacgcgc
     8761 ctaggctctg tgagaagacg gacagattac ttgaacaggc ggggcagaca ggaaagcgac
     8821 cgattgatca cgtcgtcgga cggaccgacc agcgaggtgg cgggatagtg ccgcatctcc
     8881 cagagcttga cgagtcgcac cggcaggaaa aacaatccgc cccctagcag aaacaggacc
     8941 catccagcca caagcatctc cgtaccaccg gcgagccagc aacgcttcga cagcaggcca
     9001 cagaatagcc ccaaggcgac caggccttcg agcagccaga cgaactggct cagagtcaat
     9061 cgcagaggaa gccgccgggt cgcgctgaga tagatggcat ggggcccctt aggcaacggc
     9121 gtcagtaatg cgtgaccgat gcggtggatc agtgcgctca aggaaaagcc gatggcgcta
     9181 tgaaagagaa tacttgtcag cggctcgtga gccataactg ccgcggggcc gatcatgaat
     9241 aacaggggcg cagcgtagca gagccacgcc atcacgtcct cgtaggtgaa cgttcgcaac
     9301 agatcgcggc aaccttgcgt aaacgcatct ggcctgggag cgcgattata ttccataaca
     9361 gtggtaggcg gagcgtgacc gatagaacgg agaggtgtaa atcggtgagt tcctggcctt
     9421 atctcgagca gaatgatgga tgtatacgaa cttgctgttc aggtcgtttg tatcgtggtg
     9481 ataccgaaac gtcaaacaaa gaaatgtttt cgtcgaaatg aaacctggca ccggcacatg
     9541 gtttcgatcc aaaaggccgc ctgaccgact agggagaagc cttcccggcc ccagggattc
     9601 cgctagtcgt gacttccgac gacaggcggt agaacaacat acaggcaggt tgatcgctct
     9661 tcatcacggg aggaggacac catgaaacag cagatccagc gcccggttcg acgcaccgaa
     9721 caacagaagc ccacccaaga taaagatggt gtctggcccc tgtcggcacg cgagttcatg
     9781 aagcttcccg tgtcgggact gctctcgacc gggaaacgca tccggttcac gcgcctgacg
     9841 cctcccgatc aagcggccta aggcgcggga aaggattgag aggtgcacca tgctcatcga
     9901 agacagtctc ctcacacatg acgaacgcaa ggccgccgag gccgccttcc gcggccttcc
     9961 cctggacgca caatggtcca gaggggcgca gaccatttac atcggcattc tcgaagtgac
    10021 tcgggggcgc gatatcgtgg gagatgacct gtgggaagag gtggccgccg ccgtctgatt
    10081 ccagggcgat cccgcgccgc cgagccgcag gccccgctga ccatcacgcg cggggcctgc
    10141 agagcctatc gtcattcgtc atatttccga gcattgccgt acgacttttc taccggatac
    10201 ttcgcttcgt tctttttcag tttcgcacgc acggcctcat tcacgtcgag accgaggtcg
    10261 tgacagagat aggaaagcag aatcaccaca tcggcaattt cgtccgtgac gcgttcacgg
    10321 gacgggcccc cgagcagccg cgccacttcc tcgtgtgttt tccattgaaa gacttccagc
    10381 agttcactcg cctcaaccgt aatggcggca gcgagttcct tgggcttatg aaactgctcc
    10441 cagtcacgct tccgccgaaa ttccaatacc tccgccatca cctcagcgtt caacatcact
    10501 gacgctcctt atatgcgtaa cgaaaccaat cccgtatccc agtcactctt gtctacccgc
    10561 tgtcggcacg cgccgctctt gcgggcagtg tgcgaccttg ccctgattgt ttgcaagcgc
    10621 cggcctgctg cccgacagcc gtgacacaca atccgaccga atgattgaaa tcgcgcggcg
    10681 agtggcgtag agaattggca cgggaggtcc catcatgaac cgacggctgt tgtccgcctt
    10741 gtctctcctg ctgatgctca ctgcgccggt ctatgcccaa agcccctgct ccgattgctt
    10801 ctacgctgcc gaagaagagg tgcgcaaatg cctcgataac gccatcagcg cgggcgatcg
    10861 gaatagctgc ctggacaacc ggcgggtgcg cctcaaggcc tgtagcgaca atcaatgcca
    10921 gaccgtcagg gaggaaacgg cgatcacgga gacgcaatcg acagtcagcc ggcctggcgt
    10981 cgcgccctac acgccgacgg aagccgaatg gctggccttg atcacgaggg ccggcctccg
    11041 acgggaagcc acaccggacc gcccctactc gctcgacatt attctcgccg atccacagac
    11101 tctgcagatt atggttcgac atgcgtccac aatggatcgg gaacaactgc ggaaagccat
    11161 cgaaggcgca agggagacga tccggaacac ggccaaaagt tatggctggg acaagtgggt
    11221 caaaatacga gagtcggtgg aaatgtatgc ggcaaaaaaa tgaatccggt gcggctacgc
    11281 gtctgacggg tcactccata atcagcatga tttgcgcggg cgtgagcagc gcgcctggtt
    11341 gatggcgccc cagccaggcc agggcgtccg gtccggacag tcccaacacc gatcgggcga
    11401 gcaacgtggc aaacagggcc gtgcggccga gtccggcgga acaatgaatc actacgttat
    11461 ggccctggac ggcctcttca tggacccgct tcagcacctc aggcaggccg ccatcgacgg
    11521 gaatgccata gttggggatc ggcagcggca acaccgtcag gccttcttcc cgatacatcg
    11581 ccaatagatc ccgtcccgtc ttttcccggc attcgtccgg ctcagcgagc agcaccacgc
    11641 catgaatggc cgccgctctg aacttcggca gcaacgtccc ttccggatca tagaggccga
    11701 atggcatggc gctgccgtag atgcgaccct ggagattgag cgggtatgac gtcagcgtcc
    11761 tcgcgtggct ctgctctcgt gatggattac tcagcatggt gttccgccca gtccttccag
    11821 tccgcgaccg tcgtctttag catcgccttg gccgctttct tcagccaggc agattttcgc
    11881 gctgccaggt cgcgctgaac agccttgatc tgcgcccggt cgccgagatg tacgttcgcc
    11941 gtctcccagc ccatcgcata cagcagcttg ccctcgtccc gctttttcgg taacgccgtc
    12001 acttcaatcc gggagcaata gggcgacagc cgccggagca accaggagcc gtccatcccc
    12061 acgaacggat cgcggcaacg cacggactgc tccagcatca attggtacag caacatgccg
    12121 attggcttct gccctcgcgc ccacagaccg gcggacgcaa tgagccgctt ggcttcgcga
    12181 gcgatttttc ccccgcgcca gtcggccagc gccaccacac gcggacgccc caggctgccg
    12241 agccccgata cccgccgtcg cagaccatac gagaggcctg cttcgggcat gaccttctcg
    12301 agggcgttcg ttacctccag agggactgtg tctttgagca tcggcagagc cgccatcttt
    12361 ttccagaact tcaccggatc gcgcagggaa ttcgtggcaa tgtcccgcaa ccaggcgtgc
    12421 tgctccgaca acacaaaggg ccgtccgccg gcgcgcaggc cttcgaggta gccggtcagc
    12481 accgcatcgc aggcctcatc caacttcacg gcgagatgtt cagcccgacc ggcgagtttg
    12541 gcgctgacga ccaaccgcac caggtcactg gcatagggaa agacgcaggc ctcgtcaaaa
    12601 tcgttgatgc cccagatcaa gcggccttcg ctgtcccgcc acgtgccgaa attttcgaca
    12661 tggagatcgg cgactgcccg cagcgccgga gccgcggtga gatcggcaca gacttccggc
    12721 cacacctgca gccaccggta acaggtcgcc cgcagaaacg aaaaggcgct ctgcgtcatc
    12781 atccggtgct tcagacgcag atcggccggc acaaacggca cctgccgcgc tgaccatcgc
    12841 tcatactcat gcgtcgcctg aagaatattc ataggtgctg cctgctcagt ggcgctaagg
    12901 tctcacgtct tcagtctgat cgcaagacgg gaatcgctcg taggaggaca gcaggatagg
    12961 aaggacgtca ctggacctga tgggatcaca cagcgggtct tagagtttgg ggatgtgaag
    13021 ggtcttgctg atcatgagcg taccggaaag gatgaacagc agagacaagg catgcaggtc
    13081 gccaggcccg atcgtccaca cacctccgta gagctgctcg ccgagccgat cctgccacgc
    13141 tgcccaggcc agcactccgg tcaggagcgc cgtggtcggg atgggcgtgc cttcgaaata
    13201 ggccaccttg tcatggccgg cagaaaggcg ttcggcggtc acgttatagc gggccagccg
    13261 gctcacgcca cagcagacaa aatacgtcag tgcgatccag tcccaccctc cccgcagacc
    13321 tgcggcaaag gcgagcgcgg cgggtgccac gccgaacgag atgatgtcgg ccaaggaatc
    13381 caattcccgt ccaagcaccg actgctgatg ccgccaccgg gcgacacgac cgtcgaacca
    13441 atcgaacagc agcgcggccg gcgacaacgc cgcagcagcc aaaaagtgag ccagcgaatg
    13501 gctgctcatg tactgcatcg caaagaacac acccgcgagc ccacaagcgg cattgccgag
    13561 tgtgagcaca tcggcgagat gaaactcacg gagcatcgaa aaatgtttgg gtggcaacac
    13621 agtcacggta ggaaaaccca agcgggattc cgctgcatgg tccacgtgga aacttgcccg
    13681 gccggttttc atcatcggcc actcgacagg aaaaataata gcagattact cacgattgag
    13741 aaacggcatg cacagcgccc atgggcggca ggcctgcaga cctgccgccc cgaccaccag
    13801 cgatgacgac tacttctcaa tccgctgtag cgtggtgacg tggcctttat cggtgacata
    13861 ggccttcacc atgtcgcctt cgaccacttt atcgagcttg gtgctcttgt ccacatgcac
    13921 ccggacgagc tctccatccg tattcctgat ctggtaaaac tccccgtcca tcttcatcag
    13981 cgtgcccttc acggcatcct tcgtgatccg ctcgcccagg gtcggcgacg cttccttgtc
    14041 cttcatagga tcggccgcaa acgcgaagcc tcctccaccg gtgagcaacg cggcaaccag
    14101 acacatttga agagttcgct tcatcataca acctcccatt tcgttagccg aatcgataga
    14161 acctgattca gcctaacccg cgtctggaag ccgacacact ggggaaagcc cctgtcgcag
    14221 ggagcaatgg cacgaagtca ggcgatggat gaatggaaag gagcacttcc cgattcacgc
    14281 cacggcggtt ttcaaatctt ggacaaggag agcaaagagc cgatcctgta tcacccgatc
    14341 ccgggcgcga agaatactgg acggatggta cgtgagcaga atgcgggacg aga
//
LOCUS       DAOQEW010000091        14304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1194_length_14304_cov_8.127448, whole genome shotgun sequence.
ACCESSION   DAOQEW010000091 DAOQEW010000000
VERSION     DAOQEW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14304
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1194_length_14304_cov_8.127448"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLS35_10905"
     CDS             complement(<1..262)
                     /locus_tag="PLS35_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNI19219.1"
                     /translation="MATESKEKKSTAAKNFQPLGDRLFVTYTEEMERTSGGIYVPDSA
                     KEKPQRGIVQAIGKKVENIKVGDQVLFDKYSGSKLRIEDEECL"
     gene            complement(576..2135)
                     /locus_tag="PLS35_10910"
     CDS             complement(576..2135)
                     /locus_tag="PLS35_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19220.1"
                     /translation="MTVSGCKTGASIRIVLTILMLTTLVESGCPRPSHAASLITQENA
                     RPGQSDWVLTDPADHEVEGYASATSIQRGEPLRLYIHSTDPHVTVDIYRMGWYGGTGA
                     RLVRGGITLPGARQPMPTPDPVTGLIECRWQVSYTLTTETHEKNDWLSGVYLAKLTGS
                     ASGKQSYIIFVVREDDRPSEFLFQSSVTTFQAYNNWGGKSTYPSNSRDEQWARKVSFN
                     RPYARSQHPQGGSGIGAGEFLTAMSIHPTRALSTAGWEYNMVRWLERHGYDVTYSTDI
                     DTHTRAEFWKGHKTWLSVGHDEYWSAEMRRHVEEARDHGVGLGFFSANTCYWQIRLEP
                     SLLTGEPNRTMVSYKEVAWNEDPLALDGDPSNDQLITVQWRESPVQRPEQALLGVMYG
                     TVPVDGDILITQPEHPLFNGVDLPPDHRLPGLLGYEIDQVFANGPAGLTVLAHSSYPR
                     DGEAVYGDMTLYQAQSGAFIFAAGTIQWSWGLDDYNAPTLRTARSSQAAQRITGNILR
                     LLASPASSPAH"
     gene            complement(2116..3147)
                     /locus_tag="PLS35_10915"
     CDS             complement(2116..3147)
                     /locus_tag="PLS35_10915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HNI19221.1"
                     /translation="MRQRKPKHNTDSDSDSAAELDEDDSLEPEALEPTDEEVTEEHEA
                     AERQPSKESPSAPLSTSLVPVTTLQQYLTEIRRYPYLSKEEELRLFEEYQLRGSRDAA
                     MKLILANLRVSVSIASEYLHTGADHMDLIQEGNVGLLQAIKKFDPTKNVRFYAYAAWW
                     SRAYILRYLLNTYRLIKVGTTQDQRKLFYNLKKEKAKLEREGFAPDSKLLADRLNVRE
                     RDVIEMDQRLGSWELSLDQPIGQEHDGTLMDVLPAHEQPADEQLADTQLKALFRRKLA
                     EFTKTLDEREEDILRNRILSETPLTLEDMGAKYGITKERTRQLEARIIKRLRDFIKKD
                     VKDFDRLRM"
     gene            3316..4557
                     /locus_tag="PLS35_10920"
     CDS             3316..4557
                     /locus_tag="PLS35_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI19222.1"
                     /translation="MKSLGNVRTLGTALRSGHWPTLAGAWLHLTVSFMVWVLFGALAV
                     PMGADLHLTATQQSVVVALPLLSGALLRIVTGWACDWSGAKRTGLWVLGLQLIAVLWA
                     ALSGTQYVELLAIALLLGAGGASFAVAMPVASRAYPAAHQGLVLGLVASGNIGTVLVL
                     LLAPQWEGQLGWHGACGIMALPILLAIGLFARVVREEPHTISVAEDAWWQQAWGMVQV
                     PSVYWLCLVYAITFGGFVGLTSFLPVLLHDQYGSDPIVGGAIAALCGLTGSLIRPVGG
                     YVADRWGGLPALAGVFMVLAAVTAFAASLPLLPWLASLLMVTIAVMGFGNGVIFQIVS
                     EWFPKDIGLASGLVGAAGGIGGFFVPIGFGLLREAMGTFVPGFLGLALVSAAAAMTVG
                     SALRWRPRAVASATNAEGFSR"
     gene            4622..5830
                     /locus_tag="PLS35_10925"
     CDS             4622..5830
                     /locus_tag="PLS35_10925"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNI19223.1"
                     /translation="MATPFNSIEEAIRDIKKGKFIILVDDEDRENEGDLVMAASKVTP
                     QAVNFMAKHARGLICLALTPERVEELQLAPQAAENTATFGTAFTVSIDARKGITTGIS
                     AADRATTIHVAIDPRCSPADLARPGHIFPLKAQVGGVLRRAGQTEGSVDLARLAGLYP
                     AGVICEIMNEDGTMARVAELTKFAKRHKLKMVTIKALIEYRMHRETFVRRAASAFLPT
                     TFGDFEAIAFENDIDGATHIALVKGQVDADTPMLVRVHSGCLTADVLGSLRCDCREQL
                     HRAMEIIQKDGRGVLLYLNQEGRGIGLLNKIRAYGLQDSGSDTVEANLQLGFKADLRD
                     YGVGAQILVQLGLHKIRLITNNPRKIVGIEGYGLKVVERVPIEIAPRADNEKYLRTKK
                     AKLGHLLKKV"
     gene            5980..6519
                     /gene="ribH"
                     /locus_tag="PLS35_10930"
     CDS             5980..6519
                     /gene="ribH"
                     /locus_tag="PLS35_10930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNI19224.1"
                     /translation="MKLRKGSQDATGLTVGIVASKFNKFVTSRLLTECVKALTKAGAS
                     DEAIEVVRVPGAFEIPLVARQLARSGRFDAVICLGAVIRGDTPHFEYISTEVSRGILQ
                     ASMESNLPVIFGVLTTDTVAQAIERADPAKLNRGADAAKTAIEMVNVMRQLKKEESED
                     VPPGPAPRRAGRTAARAKR"
     gene            6533..6985
                     /gene="nusB"
                     /locus_tag="PLS35_10935"
     CDS             6533..6985
                     /gene="nusB"
                     /locus_tag="PLS35_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNI19225.1"
                     /translation="MASRHQARERAVQILFQYDIHGQAGPWLDDFWTQYPLNDELRVF
                     AERLVAGVREHKKELDALIGTHATNWKVGRMPIIDRNILRLGCFELLFVPEVPAKVTL
                     NEAIELAKSFGDEEASKFVNGILDKVLGSDSRLERKRAFPELPVVGSD"
     gene            7082..8416
                     /gene="purB"
                     /locus_tag="PLS35_10940"
     CDS             7082..8416
                     /gene="purB"
                     /locus_tag="PLS35_10940"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNI19226.1"
                     /translation="MIERYTRPRMSAIWDLTHKYEIWLEVELQACAAFERAGQIPRGT
                     SAKIRKKAKIDVARIAEIEKVTKHDVIAFLESLMASVGPEHRYLHMGLTSSDIVDTSL
                     AVQMTEALDLILEDLDELIAVLKKRALEHRHTVMVGRSHGIHGEPVSFGFKLAIWYEE
                     ACRHRTRLEAARKDIAVGKLSGAMGTFAHQGPEIEEYVCAKMGLASDPVSNQIVQRDR
                     HAAYASALALLAATIEKIATEIRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPIASEN
                     LCGLARLVRSNSLAAMENVALWHERDISHSSVERVIMPDSTILVDYMLARVTDLVRNL
                     VVYPERMQRNLDLTGGLVYSQRLLLALIDKGAQRKESYEAVQRNAMTAWKGGVGFQEL
                     VNRDPFITTHLKPSEITACFDPMYYLRHLDQVFRRVFGALAIPAARRRKRRA"
     gene            8413..9210
                     /locus_tag="PLS35_10945"
     CDS             8413..9210
                     /locus_tag="PLS35_10945"
                     /inference="COORDINATES: protein motif:HMM:NF018484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNI19227.1"
                     /translation="MMAALIMRIVLMVALVFGAAVSPVGATEREALLTQPGVYGTFAA
                     FQMDHDWWDLNGEARVIAVSEVKGLIEQFSQKIVIESYLLRGLSDHADLMFRIHAHAL
                     SDTQQFLTSLLGTRLGRHLVATTYLHGMTKPAAYAAGFPESMKQALQSPAPETGTKSY
                     AIVIPIRKDADWWALDQDSRARLMQEHTQAALPYINTVKRKLYHSSGLDDFDFITYFE
                     TDKLEEFHSLIRALEQVKEYRHDRRFGHPTLLGTIRPLDDILELFAR"
     gene            9225..9938
                     /locus_tag="PLS35_10950"
     CDS             9225..9938
                     /locus_tag="PLS35_10950"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNI19228.1"
                     /translation="MTGEPAGTMLYEGKAKKIFTTERPDQVLQYFKDDATAFNAQKRG
                     TIVDKGVVNNKVSERLFKLLEQQGIQTHFIERVSDREMLTKKVTIVPVEVVVRNVVAG
                     SLAKRLGLKEGAAIEPAIIEFYYKDDALGDPLVNDDHLRVLNVATPAVLREMRELGHK
                     INAVLLPFFKERRMLLVDFKLEFGVFHNRLILADEISPDTCRFWDQATKESMDKDRFR
                     KDLGKIEEAYQEVLKRVCE"
     gene            10058..10282
                     /locus_tag="PLS35_10955"
     CDS             10058..10282
                     /locus_tag="PLS35_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19229.1"
                     /translation="MNRFLRFFLDYGLVLAILVWAATVALMAYHLDESPWRWAFILLS
                     LAGLGTIGVIFWIRTYVNKTAQTQRVGKVQ"
     gene            10279..10518
                     /gene="purS"
                     /locus_tag="PLS35_10960"
     CDS             10279..10518
                     /gene="purS"
                     /locus_tag="PLS35_10960"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNI19230.1"
                     /translation="MKAKIHVTLKQGILDPQGKAIEHALDSLGFKNAANVRVGKYMEV
                     DVNETDSAKADAQVKQMCEKLLANTVIEEYRYELQ"
     gene            10636..11358
                     /gene="purQ"
                     /locus_tag="PLS35_10965"
     CDS             10636..11358
                     /gene="purQ"
                     /locus_tag="PLS35_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNI19231.1"
                     /translation="MNIGVVVFPGSNCDHDCEYIFKDVLGQSVKMIWHKDTLPAGLDA
                     VVLPGGFSYGDYLRTGAIARFSPVMGAVKTFARDGGLVIGICNGFQILLEAGLLPGVM
                     LRNTSLNFICKDVYVKVENAATRFTNRCESGQVLKIPIAHADGNYYTDPVTLGGIKAN
                     AQVIFRYCTAEGKITPEANPNGSLDNIAGIMNADGNVLGMMPHPERSAETLLGNEDGR
                     LIFLSMLSAFGKSEQPSRLVGV"
     gene            11426..13672
                     /gene="purL"
                     /locus_tag="PLS35_10970"
     CDS             11426..13672
                     /gene="purL"
                     /locus_tag="PLS35_10970"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNI19232.1"
                     /translation="MKSLVITQAILEQHKLSEEEYKKILEILGREPNWTELGMFSAMW
                     SEHCSYKSSRIHLKKLPTTGPRVVQGPGENAGAVDIGDGLCAVFKMESHNHPSFIEPY
                     QGAATGVGGILRDIFTMGARPIALLNSLRFGGLDNANTRHLLKGVVSGIAGYGNCMGV
                     PTVGGEIVFNDIYALNPLVNVFCLGIARKDKIFLGKAAGVGNPVIYFGSKTGRDGIHG
                     ATMASDSFDEQAAQKRPTVQVGDPFTEKLLLEACLELMAGDCLVGIQDMGAAGLTSSA
                     CEMASREGTGVELELADVPRREPGMTPYEIMLSESQERMLMVAKAGREDEVIKICRKW
                     DLDVAVIGRVTDTGKVVLKENGQIVAEIPAKALADDGPRYDRPSAPPAYQEMLQSLNY
                     DALPDVKDANATLLELLGAPTIASKRWVYEQYDHMVRTNTMVRPGSDAAVLRVKGTNK
                     ALALSVDCNGRYCLLNPYEGAKIAIAECARNLACSGAEPIGVTDCLNFGNPQRPEVMW
                     QFVLAIEGLKDACEALSVPIVSGNVSLYNETKDLSIYPTPMIGMVGLIEAADRTATQW
                     FKQAGDVIILLGRTREDLGGTEYLKIVHHREQGSPPLLNLEDERAVQACTIGLIREGL
                     VQSAHDCSDGGLAVALAECCVSGPNGTVGAVVQLPLDGLRRDALLFGESQSRVVLSVK
                     QEQVDNVLNAARNAGVPASRIGVVGGSRLVIRVDGDQRVAGCTIDLEVTSVQERWALA
                     IPRALGQD"
     gene            13685..>14304
                     /locus_tag="PLS35_10975"
     CDS             13685..>14304
                     /locus_tag="PLS35_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNI19233.1"
                     /translation="MAKELPLIASDQFHDECAVFGIYGHKEAANLAYLGLYALQHRGQ
                     EASGIVSNDGEQFYVEKGQGHVADIFSQQALARLPGAMAIGHNRYSTAGGAGLKNVQP
                     LSVNFAFGNLAVAHNGNLINATMLRSELEAYGAIFQSTSDTEVIIHLIAHSRADTLLD
                     RVIDSLTQVRGAFSVVLMTDQGVIAARDPHGFRPLCLGRFRDAWIVA"
BASE COUNT         2907 a         3774 c         4453 g         3170 t
ORIGIN      
        1 tgaggcactc ttcgtcttcg atccgcagct tgctgccgga atacttgtcg aacaacacct
       61 ggtcgccgac cttgatgttc tcaactttct tgccgatagc ctgcacgatt ccccgctgcg
      121 gcttctcttt cgcggagtca ggaacataaa ttccgccaga tgtccgttcc atctcctcgg
      181 tgtaggtgac gaacaggcgg tcaccgagcg gctggaaatt cttcgcagcg gtggacttct
      241 tttccttcga ttccgtagcc atacccaatc ctccttctag attaatgagc gtcgaggccc
      301 taatgtacag ttgtgtgaaa gctggaaatt cagctctgac gtgttactgc aagggcaacc
      361 tgcagaaaac tcgagtcata gatagcgcta tgacgaggga agtcaatgga tggactataa
      421 aattattctt ccgcgagatg caatgggaaa gtgccgcgca agttgttgat tctacgaatt
      481 tcgtagccca tctcctcgca gccgtcttct gaagcacatc gtagggcctg accggaaccg
      541 aacgtcggcc gtcgcagtga agccgcccgg tgtcgctaat gagccgggct ggaggcgggt
      601 gaggccagca atctcaatat gttgccggta attcgttgcg ccgcctgact ggatcgggcg
      661 gtccgcaatg tcggggcatt atagtcgtcg aggccccagc tccattgaat cgtcccggca
      721 gcgaatatga aagcgccgct ctgggcctga tacagggtca tgtcaccata aactgcctcc
      781 ccatccctcg gataggagga atgagccagg acggttaaac cggctggtcc gttggcgaat
      841 acctggtcga tttcgtatcc caggaggccg ggaaggcgat ggtcgggcgg cagatccaca
      901 ccgttgaaca gcggatgctc gggctgcgtg atgagaatat ctccatcgac cgggacggtc
      961 ccatacatga cgcccagcaa tgcctgttcc ggtcgttgaa ccggcgattc acgccattgg
     1021 acggtgatca actggtcgtt tgatggatcg ccatccaggg ccaggggatc ctcgttccat
     1081 gccacttctt tatagctcac catggttcta ttcggctcgc cggtaaggag gctgggctcc
     1141 agtctgatct gccagtagca ggtattggca gaaaaaaaac ccaggccgac tccgtggtct
     1201 cgagcctcct cgacatggcg tcgcatctca gccgaccagt attcgtcatg ccccacggac
     1261 agccaggtct tatggccctt ccagaattct gcccgggtat gggtgtcgat gtcggtgcta
     1321 taggtcacgt cgtatccgtg acgttctagc caacgcacca tgttgtactc ccagcccgcg
     1381 gtcgagagcg cgcgggttgg atgaatcgac atcgcggtca ggaattctcc cgccccaatc
     1441 ccgcttccgc cctggggatg ttggctcctg gcatagggcc ggttaaaaga gacctttcgt
     1501 gcccattgct catcccggct gttggacgga taggtcgatt tcccgcccca attgttgtag
     1561 gcctgaaagg tggtgacgct ggattgaaag agaaactccg agggccgatc gtcttccctg
     1621 accacgaaaa tgatgtagct ctgtttgccg ctggccgagc cggtgagttt cgcgaggtac
     1681 acgccgctga gccagtcgtt cttctcatgt gtctctgtcg tcagggtgta tgagacttgc
     1741 catcgacatt cgatgagacc ggtcacggga tccggtgtcg gcatgggttg tcgagcgcct
     1801 ggcagcgtga tgcctccccg caccaatcga gctcctgtcc cgccatacca tcccatacgg
     1861 tagatgtcga ccgtcacatg gggatcggtg ctgtgaatgt agaggcgaag cggttctccg
     1921 cgctggatgc tggtggccga ggcataaccc tcgacctcat gatcggcggg atcggtcaga
     1981 acccagtcgg attgaccggg ccgggcattt tcctgggtga tgagggaggc ggcgtgggaa
     2041 gggcgcggac accccgattc gacgagggtc gtaagcatca gaatggtgag gacgattctg
     2101 atgctagccc cggtcttaca tccggagacg gtcaaagtcc ttcacatcct ttttgatgaa
     2161 atcacgcagt cgtttgatga tgcgggcctc cagttgcctg gtccgttcct tggtgatgcc
     2221 gtactttgcg cccatgtcct ccagcgtgag tggagtttcg ctcagaatgc ggttgcggag
     2281 aatgtcttcc tctcgctcgt cgagcgtttt ggtaaattcc gccagcttgc gccgaaagag
     2341 tgctttcagc tgcgtgtccg ccagttgctc gtctgccggc tgttcatgcg caggcagcac
     2401 gtccatcaac gtcccgtcat gttcctggcc gatgggttga tccagcgaca attcccaact
     2461 gcccagccgc tgatccatct cgatgacatc gcgttcacgg acgttcagcc ggtcggccag
     2521 cagcttgctg tctggcgcga agccttcgcg ttcgagcttg gccttttctt tcttcagatt
     2581 gtagaacagc ttgcgttgat cctgggtggt gccgaccttg atcaggcgat aggtgttcaa
     2641 gaggtagcgg aggatatacg ccctggacca ccaggctgcg taggcataaa agcgcacgtt
     2701 tttcgtcggg tcgaatttct tgatggcctg caggaggccg acgtttcctt cctggatcag
     2761 gtccatatga tcggcgcccg tgtgcaggta ttcggaagcg atggagacgg agacccgcag
     2821 attggcaaga atgagtttca tcgccgcatc ccggcttccg cgaagctgat actcctcgaa
     2881 gaggcgtaac tcttcttcct tgctgaggta ggggtagcgg cggatttccg tcagatattg
     2941 ttgcagggtc gtgacgggga ccagggaagt gctcaatggt gcagacggcg actccttcga
     3001 tggttgccgc tcggccgcct catgttcttc cgtcacctcc tcgtccgttg gctccagggc
     3061 ctcgggttca aggctgtcgt cttcgtccaa ctccgcagct gaatcggagt cagaatcggt
     3121 attatgcttt ggcttgcgct gtctcatgcg agtcgaaccc tttgcataca ggagctgact
     3181 ataccagatg tcgagtgttg ggaaacaatg agtacgtctg atctgcatcg gccttgcttg
     3241 cgtctctgaa atcgcgcctc gtatagtgcg tgatgatgaa agagattcgt aaggctcgga
     3301 ccctggccgc ataagatgaa gtcgttagga aatgtcagaa cattgggaac ggctctgcgt
     3361 agcgggcatt ggccgacctt ggcaggtgcc tggttgcatc tcactgtgag ttttatggtg
     3421 tgggtgctgt tcggggcgct ggcagtgccg atgggcgcgg acctccacct gacggcgacg
     3481 caacagagtg tggtggtggc gcttccgctt ctgagtggtg cgctgctgcg aattgtgacc
     3541 ggctgggcct gcgactggtc tggagccaaa cgaaccggtc tgtgggtctt ggggttgcaa
     3601 ctgatcgcag tcctctgggc ggcgctcagt gggacacagt acgtagagct gctcgccatc
     3661 gcattgcttc tgggtgccgg aggagcaagt tttgcggtgg ccatgccggt ggccagtcga
     3721 gcgtacccgg ccgcccatca aggcctggtg ttgggattgg tcgcgtctgg caatattggc
     3781 acggtgttgg tccttctgct ggcgccgcaa tgggaggggc agttgggatg gcacggggcc
     3841 tgcgggatca tggcgctgcc aattctgctg gccatcggtc tgttcgctcg tgtcgtgcgg
     3901 gaagaaccgc atactatctc cgtcgcagaa gatgcctggt ggcagcaggc ctggggaatg
     3961 gtgcaagtgc cctctgtcta ttggctctgc ctcgtctatg ccattacgtt cggcggattt
     4021 gtcggcctga caagttttct cccggtgctg ctgcacgatc agtacggcag cgatccgatt
     4081 gtcggcgggg ccattgccgc gctctgcgga ttgaccggca gcctgattcg cccggtgggc
     4141 gggtatgtgg cggatcgctg gggcggcctc cctgcgttgg cgggtgtgtt tatggtgctc
     4201 gctgccgtca cggcattcgc cgcttcgttg cctttattgc cctggctggc gagcctgctg
     4261 atggtgacga ttgccgttat ggggttcggc aatggcgtga tctttcaaat cgtctccgag
     4321 tggtttccca aggatatcgg gctggcatca ggcctggtgg gggccgcagg ggggatcggt
     4381 ggattttttg tcccgatcgg gttcggatta ttgcgggaag ccatggggac gttcgtgccg
     4441 ggttttctcg gattggcgct ggtgagtgcc gccgccgcca tgacggtggg ttccgcctta
     4501 cggtggcgac cccgggcggt cgcatcagcc acgaatgcgg aaggtttttc ccgttgacag
     4561 gcatggtttg aaaagagtta gctgggcacc cacgcaggcc ggtcggtaca attgagggac
     4621 tatggcgaca ccgtttaatt cgatcgaaga agccatccgg gacatcaaga aggggaagtt
     4681 catcatcctc gtcgatgatg aagatcgcga gaacgaaggc gatcttgtga tggcggccag
     4741 caaggtcacg ccccaggcgg tcaatttcat ggccaagcat gcgcgtgggt tgatctgctt
     4801 ggccctgacg ccggaacggg ttgaggaact gcagttggcc ccgcaggctg cggagaatac
     4861 ggccaccttt ggcaccgcct tcacggtgtc gatcgatgcc aggaaaggta tcacgacagg
     4921 catttcggca gccgaccgcg cgacgaccat ccacgtggcc atcgatcccc gttgttctcc
     4981 ggctgatctg gcgcgtcccg gccatatttt tcctctgaag gcgcaagtcg ggggtgtgct
     5041 ccgccgtgcg gggcagaccg aagggtcggt ggatctggct cgactggccg ggctctatcc
     5101 cgccggagtc atttgcgaaa ttatgaatga ggacgggacg atggcgcgcg tggccgagct
     5161 gaccaagttc gccaaacgcc acaaattaaa aatggtgacg atcaaagcct tgatcgaata
     5221 ccggatgcat cgagagacct tcgtgcggcg ggcggcgagt gcgttcttgc ccacaacgtt
     5281 cggtgatttt gaggcgattg cgttcgagaa tgatatcgac ggggcgactc acatcgcctt
     5341 ggtgaaggga caggtcgatg ccgacacgcc catgcttgtc cgggtccatt cgggatgcct
     5401 gacggcggat gtgctgggat cgttgcgctg tgattgccgc gagcaactcc atcgcgccat
     5461 ggagatcatc cagaaggacg gccggggagt gttgctctat ctcaaccagg aggggcgcgg
     5521 aatcggcctg ctgaataaga tcagagccta cggccttcag gattccggca gtgatacggt
     5581 cgaggccaat cttcaactcg gcttcaaggc ggatttgcgg gactatgggg tcggcgcgca
     5641 gatcctcgtc cagctcgggc tccataagat ccgcctgatc accaacaatc cgcggaagat
     5701 tgtcgggatt gaagggtacg ggttgaaggt ggtcgaacgt gtgcccattg aaattgctcc
     5761 acgcgctgat aatgaaaaat atctccgcac caaaaaggcc aaactcggcc acctgttgaa
     5821 aaaagtctga cgcgtctgcc gcgctttatt tcgtacgtcc ttcgccagga agagatgcgc
     5881 ctgaaacgtg tagccatttg atggcgtttc caggtatgat cgccctcttt cttcggcgca
     5941 cgcgcaatcc atgaaacgtc tgaatcggta tcgcacggca tgaagcttcg caagggctcc
     6001 caagacgcga cgggtctgac cgtcgggatc gtggcgagca aattcaataa gttcgtcacc
     6061 agccgcctgc tcactgaatg cgtgaaggcg ctgaccaagg ccggagcgtc ggacgaggcg
     6121 attgaggtcg tgcgggtgcc tggtgcgttc gaaattcctc tggtggctcg ccaactcgcc
     6181 cggtctggtc gattcgacgc agtcatctgc ctgggcgccg tcattcgagg cgacacacct
     6241 cattttgaat acatcagtac cgaagtaagc cggggtatcc tgcaggcctc gatggaatcg
     6301 aatctcccgg tgatttttgg ggtgctcacg accgatacgg tggcgcaggc gattgaacgt
     6361 gcggatccgg cgaagctcaa tcgtggtgcc gatgccgcca aaacggccat tgaaatggtc
     6421 aacgtgatgc ggcaactcaa aaaagaagag agcgaggacg tgccgccggg ccccgctcca
     6481 cgaagagccg gacgaacggc cgcgagggcg aaacgctgac cggcgttgtc ttatggcctc
     6541 tcgacaccaa gcccgtgaac gggcggtaca gattctcttt caatacgaca ttcatggcca
     6601 ggccggtcca tggctggacg atttttggac gcaatatccg ctgaatgatg agctccgcgt
     6661 cttcgccgag cgcctcgtgg ccggggtcag agagcataag aaagaactgg atgcgttgat
     6721 cggcacccat gccaccaatt ggaaggtcgg ccggatgccc attatcgatc ggaatattct
     6781 tcgcctgggc tgcttcgaac tgctttttgt ccctgaggtt ccggcgaagg tgacgctgaa
     6841 tgaagcgatc gagctggcga aaagtttcgg cgatgaagaa gcctccaagt ttgtaaatgg
     6901 aattctcgac aaggtgctgg gatcggatag tcggctggaa cggaaacgcg cgttcccgga
     6961 attaccggtt gtcgggagcg attaggtcgc ctggaccgta caggtcagac gatgagaaga
     7021 cgatggaagc tgaaaacttg aagctgtttg tgattcacgg tgttggtttc atgaggtggc
     7081 gatgattgag cgatataccc ggcctcgcat gagcgcgatc tgggatctca cacacaagta
     7141 cgagatttgg ttggaagtcg aactgcaggc ctgcgcggcc tttgaacggg ccggacagat
     7201 cccgcgtggc acgagcgcaa agatccggaa aaaggccaag atcgatgtgg cgcgtatcgc
     7261 agaaattgaa aaggtgacga agcacgatgt gatcgcgttt ctcgaatcgc tcatggcgtc
     7321 cgtcgggccc gagcatcgct atctccacat ggggctcacc tcatcggaca ttgtcgatac
     7381 gtccctggcg gtacagatga cggaggcgct cgatctcatc ctcgaagatc ttgatgagtt
     7441 gattgccgtt ttgaaaaagc gcgcgttgga gcatcgccat acggtgatgg tcggccgttc
     7501 gcatggtatc catggcgagc cggtgtcgtt cggattcaag ttggcaatct ggtacgaaga
     7561 ggcgtgccgg caccggacac gtcttgaggc ggccaggaaa gacattgccg tcggcaaact
     7621 ttccggcgcg atggggacat ttgcccatca ggggccggaa atcgaagaat atgtctgcgc
     7681 caaaatgggg ttggcttctg atcccgtctc gaatcagatc gtgcagcgcg atcggcatgc
     7741 ggcctatgcg tccgccttgg cgttgctggc ggccaccatt gaaaaaatcg ccacagaaat
     7801 ccgccatctg cagcggacgg aagtgctgga ggcggaagaa tatttttccg agggtcagaa
     7861 gggctcctcg gcgatgccgc acaagcgcaa tccgattgcc tcggaaaacc tctgcggtct
     7921 tgcgcgtctc gtgcgcagca acagtctggc ggcgatggaa aatgtcgcgc tgtggcatga
     7981 gcgggatatc agccactcct cggtcgagcg agtgatcatg cccgacagca ccatcctggt
     8041 cgattatatg ttggcgcgcg tcacggacct ggtcaggaat ctggtggtgt atcccgaacg
     8101 gatgcagcgg aatctggatt tgaccggggg gctggtctac tcgcagcggc tcctgctcgc
     8161 gttgatcgat aagggcgctc aacgcaagga gtcgtatgag gcggtccagc gcaatgccat
     8221 gacagcctgg aaaggcgggg tcggatttca ggaactcgtc aaccgggatc cgttcatcac
     8281 gactcatttg aagccgagcg agatcaccgc ttgtttcgac cccatgtatt atctgcggca
     8341 tctcgatcag gtgtttcgcc gggttttcgg cgcccttgcc atcccagccg cgcgacgccg
     8401 caagaggcgt gcatgatggc tgcgttaata atgaggatcg tgctgatggt ggccctggtg
     8461 ttcggggcgg ccgtgtcgcc ggtcggtgcg accgagcggg aggccctgct gacccagccc
     8521 ggcgtttacg gcaccttcgc tgcgttccag atggatcacg attggtggga tctgaatggc
     8581 gaagcccgcg tgatcgccgt gtctgaggtg aaggggctca tcgagcagtt ctcccagaag
     8641 atcgtcatcg agtcctattt gctccgtggg ctttcggacc atgctgactt gatgttccgc
     8701 atccatgccc acgccttgtc tgatacgcaa cagtttctca cctcgttgtt gggcacccgt
     8761 ttgggacgcc atctggtggc gacgacgtat ttgcatggga tgaccaagcc ggctgcctac
     8821 gcagccgggt ttcccgaatc catgaagcag gcgttgcagt cgccggcgcc ggagacaggg
     8881 acgaagtcct atgcgatcgt tattcccatc cgaaaggatg ccgattggtg ggcgctggat
     8941 caggatagcc gcgcgaggct catgcaggaa catacgcaag cggcgctgcc ctacatcaat
     9001 accgtgaaac ggaaactgta tcattccagc ggcttggacg actttgactt catcacctat
     9061 ttcgagacgg ataagctgga agagttccac agtttgatcc gcgcgcttga acaggtaaag
     9121 gaatatcgcc atgaccggcg gtttgggcat cccacattgt tgggaacgat cagaccgctg
     9181 gacgatattc tggaattgtt cgcacggtag ttggaggatt gaggatgact ggcgaaccgg
     9241 caggcacgat gctctacgag ggcaaggcga agaaaatttt cacgaccgaa cggccggatc
     9301 aggtgttgca gtatttcaag gacgacgcca ccgcgtttaa tgcgcaaaag cgcgggacca
     9361 tcgtcgataa aggggtggtc aacaataagg tgtcggagcg gctgttcaaa ctgctcgagc
     9421 agcaggggat tcagacccat ttcattgagc gcgtcagcga ccgcgagatg ctcaccaaga
     9481 aagtgaccat cgtcccggtg gaagtcgtgg tccgaaacgt agtggcgggg agtctcgcca
     9541 agcgtttggg gctcaaggaa ggcgcagcca tcgaaccggc catcatcgag ttctattaca
     9601 aggacgacgc gctgggcgat ccgttggtga acgacgatca tttgcgcgtc ttgaacgttg
     9661 ccacaccggc cgtactgcga gagatgcgtg aattgggcca caagatcaat gccgtcttgt
     9721 tacccttctt caaagagcga cgtatgctgt tggtagattt taaactggaa ttcggcgtct
     9781 ttcacaatcg actcattctc gccgatgaga tctctcccga cacctgccgg ttctgggatc
     9841 aggccacgaa agaatcgatg gacaaggatc ggtttcggaa ggatctgggc aagatcgaag
     9901 aggcctatca agaagtcttg aagcgggtct gtgagtaggg aactcacgag aacggccatc
     9961 cctctgcaat acggagcgat gaagtccctc cttcgacaaa cgcaacagtc ccaggcatat
    10021 cgtccccgag ggatgtcgag aaacgggacg cgacacgatg aataggtttc tgcggttttt
    10081 tttagattac gggttggtgc tggcgattct ggtgtgggct gccacagttg cgctgatggc
    10141 atatcatctg gatgaatcgc cctggcgctg ggcgtttatt ctcttgtcac tggcgggact
    10201 gggaacgatc ggggtgattt tctggattcg cacgtatgtg aacaaaaccg cgcagacgca
    10261 gcgcgtaggg aaagtacaat gaaagccaaa attcatgtca cgctgaagca ggggatttta
    10321 gatccccagg ggaaagcgat cgagcatgcc ttggactcgc tcgggttcaa gaatgcggca
    10381 aatgtgcggg tgggcaagta catggaagtg gatgtgaatg aaacagattc ggccaaggcc
    10441 gatgcgcagg tcaagcagat gtgtgagaaa ctgctggcca atacggtcat cgaagaatat
    10501 cggtatgagc tgcagtgacg tggcggctgg tgacgctgag ctcaggctct ccccgtcgga
    10561 agatgcagat cggccgtcgt tgtgcgcgca tcatatgacc ggttccggtc agctggccgc
    10621 aagaggatga gtgacatgaa catcggcgtg gtggtgtttc ccggcagcaa ttgcgatcac
    10681 gattgcgagt acatctttaa agacgtgctt ggccagtctg tgaagatgat ctggcacaag
    10741 gacaccttgc ccgcaggctt ggatgccgtc gtcttgccgg gagggttttc ctatggggac
    10801 tatctcagga cgggcgcgat cgccagattc tcccccgtca tgggcgcggt taaaacattc
    10861 gcgcgagacg gtgggctggt catcggcatt tgcaacggct tccaaattct tctcgaagcc
    10921 ggtctgttac ccggtgtgat gttgaggaat acctccctga atttcatctg caaagatgtg
    10981 tatgtgaagg tggagaacgc cgccactcgt ttcaccaatc gctgcgagtc cggtcaggtg
    11041 ctgaagattc ccatcgctca tgcagacggc aactactata ccgatccggt gacgcttggc
    11101 ggcattaagg ccaatgcgca agtgatcttt cgatattgta cggcagaggg caagatcacg
    11161 cccgaggcaa atcccaacgg ctcgctcgac aatatcgccg ggatcatgaa cgctgacggg
    11221 aatgtgttgg ggatgatgcc gcatcccgaa cgaagcgctg aaacgctgct gggtaacgaa
    11281 gacggacggc tgattttcct ctccatgctg agcgcgttcg gcaagtctga acagccatcc
    11341 agattagtcg gcgtgtgaag gcatggcgaa tgaataggag cgccggatcg accggttctc
    11401 atccacgata gagaatgtgc aagacatgaa atcattggtc attacccaag ccatccttga
    11461 gcagcacaag ctgagcgagg aggaatataa gaagatcctg gaaatccttg gccgggagcc
    11521 gaactggacc gaactgggca tgttctcggc tatgtggagc gaacattgct cctacaagag
    11581 ctcccgcatc catttgaaaa aattacccac gaccggtccg cgcgtcgtcc agggaccggg
    11641 cgagaatgcc ggcgctgtcg atatcggcga cggcctatgc gcggtcttca agatggagtc
    11701 tcacaaccat ccctccttca tcgaacccta ccagggcgcg gcgaccggcg tcgggggaat
    11761 tcttcgcgat atttttacaa tgggcgccag gccgatcgct ctgttgaact cgctgcgatt
    11821 cggcggcctg gacaatgcga acactcgcca tcttctgaaa ggcgtggtgt caggcatcgc
    11881 cggttatggc aattgcatgg gggtgccgac ggtcggcggt gagatcgtgt ttaatgacat
    11941 ctatgcgctc aatccgttgg tcaatgtctt ctgcctgggg attgccagga aggataagat
    12001 ttttctgggc aaagcggcgg gagtcgggaa tcccgtcatc tatttcggct caaagacggg
    12061 gcgggacggg atccacggcg cgaccatggc gtccgattct ttcgatgaac aggctgccca
    12121 aaagcgcccg actgtgcagg tcggcgaccc gtttacggag aagctgctgc tggaggcctg
    12181 cctcgagtta atggccgggg actgtttggt cgggattcag gacatgggag cggcaggttt
    12241 gaccagttcg gcctgcgaaa tggcttcgcg ggaaggcacc ggcgtggaat tggaattggc
    12301 ggatgtgccg cgtcgcgagc ccggcatgac cccgtacgaa atcatgctgt ccgaatcaca
    12361 agaacgcatg ttgatggttg cgaaagccgg ccgcgaggat gaagtcatca agatttgccg
    12421 taagtgggat ctggatgtgg cggtcatcgg gcgcgtcacg gataccggca aggttgtgtt
    12481 gaaagagaat gggcagatcg tggcggaaat tcccgccaag gccctggcgg acgacggccc
    12541 acgctatgat cgacccagtg cgccaccggc ctatcaggaa atgttgcagt cgttgaatta
    12601 tgacgcattg ccggatgtga aggacgcgaa tgcaactctg cttgaactct taggcgcacc
    12661 gacgattgcc agtaagcgct gggtctacga acaatacgat catatggtgc ggacgaacac
    12721 gatggtgcgg cccggatctg atgcagctgt gctgagggtg aaaggcacca acaaggcctt
    12781 ggcattgtcc gtcgattgta atggacgcta ttgtttgttg aatccctatg aaggtgccaa
    12841 gattgcaatt gcggagtgtg cacgtaatct cgcctgttcg ggggcagaac cgatcggtgt
    12901 caccgactgc ctcaatttcg gcaatccgca gcgcccggaa gtcatgtggc agttcgtgct
    12961 ggccatcgaa ggcctcaagg atgcctgtga agccttgagc gtgcccatcg tcagcgggaa
    13021 tgtaagtctg tacaacgaga ccaaagacct gtcgatctat ccaacaccca tgatcgggat
    13081 ggtgggattg attgaggccg ccgaccgtac ggccacgcag tggtttaagc aagccggcga
    13141 cgtcatcatc ttgctgggtc gaacacgtga ggatcttggg ggaacggagt acctcaagat
    13201 tgtgcatcat cgcgaacaag gatcaccgcc gttgctgaat ctcgaggatg agcgagcggt
    13261 gcaggcctgt acgattggac tcattcgaga gggcttggtt caatcggcac acgattgctc
    13321 cgatggggga ttagccgtag ccttggccga atgttgcgtc tctggtccca atgggaccgt
    13381 cggtgctgtg gtacaattgc cgcttgatgg tttgcggcgc gatgccttgc tcttcggaga
    13441 aagccaatcg cgggtcgtac tatcagtgaa gcaggaacag gtggacaacg tgttgaatgc
    13501 cgcgaggaat gccggcgtgc ctgcgtcacg catcggcgtc gtcggcggca gccggttggt
    13561 gattcgagtt gacggtgatc agcgtgtcgc ggggtgcacg atcgatctcg aggtgacaag
    13621 cgttcaggaa cgctgggcct tggcaatacc ccgggcgctg ggtcaggact agagcagaga
    13681 cgtcatggcc aaagaattac ctctcattgc ttccgatcaa ttccacgacg agtgtgcggt
    13741 ctttggcatt tacgggcaca aagaagccgc caatctcgcc tatctcggtc tctacgcgct
    13801 ccaacaccgc gggcaggaag cctccggcat tgtctccaac gatggggagc aattttatgt
    13861 cgagaagggg cagggccatg tggccgatat cttctcgcaa caggcgttag ctcgtcttcc
    13921 cggtgccatg gccatcggac acaaccgcta ttccacagcg ggcggcgctg gcctgaagaa
    13981 tgtgcagccc ttgagcgtca actttgcgtt tgggaacttg gcggttgcgc ataatggaaa
    14041 cctcatcaat gccaccatgc ttcgcagcga attggaagcc tatggcgcca tttttcaatc
    14101 gacatccgat acggaagtca tcatccacct gattgcgcac tcacgagcgg atacgctgct
    14161 tgaccgggtg attgattcac tcactcaggt tcgcggcgct ttttccgtgg tgctcatgac
    14221 cgaccagggt gtcatcgcgg ctcgggatcc gcatggattc cgtccgctct gtttggggcg
    14281 ctttcgcgat gcctggattg tcgc
//
LOCUS       DAOQEW010000092        14205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1205_length_14205_cov_9.080283, whole genome shotgun sequence.
ACCESSION   DAOQEW010000092 DAOQEW010000000
VERSION     DAOQEW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1205_length_14205_cov_9.080283"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /gene="rpsU"
                     /locus_tag="PLS35_10980"
     CDS             <1..194
                     /gene="rpsU"
                     /locus_tag="PLS35_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNI19234.1"
                     /translation="EIKVFNNNVEKALKVAKKKLAGEGLFRELKRRRYYEKPSVRKKA
                     KEREAQRRRQKWLAKRRPE"
     gene            264..923
                     /gene="nth"
                     /locus_tag="PLS35_10985"
     CDS             264..923
                     /gene="nth"
                     /locus_tag="PLS35_10985"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNI19235.1"
                     /translation="MQAHEIHAVIRTVKREIARWPDPVVGVVAKKSGRDPFLVLISCL
                     LSLRTKDKTTAEASARLFALASTPATMQQLSQSTVESAIYPVGFYRTKAKQIQQICRQ
                     LLDRYHGRVPDQIEALLTLPGVGRKTANLVVTVGYEKPGICVDIHVHRISNRWGYVKT
                     KTPDETEKALRGKLPRKYWITFNDLLVPFGQNLCQPVSPFCSRCKIAEYCDRVGVTKS
                     R"
     gene            complement(982..1401)
                     /locus_tag="PLS35_10990"
     CDS             complement(982..1401)
                     /locus_tag="PLS35_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19236.1"
                     /translation="MADKPKFVLFAHNATYDKLHQVATLGLTASAMGKDVIVVLLFWT
                     IKKLAEGKIDHIDFPPEYAASAEEVARLLKEKKVPRISEMFHEARLVGQFRLIACSAG
                     LEYMGVEAGAVERNVDEVLGLPAILSLTAQAETKLFI"
     gene            complement(1407..1775)
                     /locus_tag="PLS35_10995"
     CDS             complement(1407..1775)
                     /locus_tag="PLS35_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19237.1"
                     /translation="MLRTIRSSACRLAVLGLILVLNTVEVFDQGAQAADSAAPPELQI
                     DISKIGLGKEVVITQGNKEWFMRVEVTPENSVVVRQEKQGERYLLDESEMHDRAMTAA
                     EVDAAISDFINSVKTRQKVK"
     gene            1899..2327
                     /locus_tag="PLS35_11000"
     CDS             1899..2327
                     /locus_tag="PLS35_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HNI19238.1"
                     /translation="MSDMLAQIESYVPASVLIWFAVSSVVMFVGTLIAIPVILMRLPA
                     DYFDTRIPRPWMENHHPVLRLLGHVVKNVVGAIFLFAGFLMLFLPGQGVLTMLIGLSL
                     IEFPGKRRVEAKIVGQSTVLSTINAMRAKFDKPPLIIAPE"
     gene            2391..5036
                     /gene="polA"
                     /locus_tag="PLS35_11005"
     CDS             2391..5036
                     /gene="polA"
                     /locus_tag="PLS35_11005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNI19239.1"
                     /translation="MPTLYLIDGSAYIYRAFFALPPLSNSKGLQTNAVYGFTTMLMKV
                     LRDHHPDYVAVVFDEKGPTHRHEAFKEYKAQRPPMPQGMSAQVPYIHRVVEALSLPVI
                     RQAGYEADDLIGTLARKGEGEGLDVVIVTSDKDMFQLLTPKTRIYDPVKDKWFGEADS
                     QVRFGVEPARVVEIMGLMGDTSDNIPGVKGIGEKTAVKLITQFGTIEELLRRVEEVTP
                     AKTKNLLLEQGESARMSRQLATIQVDCPIEFAPAHFQARAPQTEVLVSLLRELEFMTL
                     AKAFQGDTPEQNRLGAEVQRIHDTAGAEDFLKRRQPDTMMGLACVLSDEAGVRADIQG
                     CAFGWPDGTAAFVHGEAQDWPQPLTAYVRDGSRPKAVQDLKPTLLALQRQGIDMPGPY
                     FDTMVADYLLNPNRRAHTLEAIAMDLLSYQLGTGEREEGGKEPQSLFDVDERVVRRTG
                     EAAAVTAKVAPMLRDRLKEQGSLSLFEQVEMPLVPVLADIERNGFLLDVEGLRTLSKE
                     LERELEQMVGTIYRLGGGEFNIGSPKQLATVLFETLGLKPLRKTKTGFSTDEDTLTQL
                     ANQHELPAQILSYRTLTKLKSTYVDALPQLVNPDTKRLHTSLNQTVAATGRLSSTDPN
                     LQNIPVKGDYGLRIREAFIAPPGHHLLCADYSQVEPRILAHLSQDPRLLEVFEKGEDI
                     HMATAMEIFNLPAGDVTREMRRAAKSVVFGIVYGISPFGLASNIGVPQAEAKKYIETF
                     FEKFAAVRALMDHNINDGKTKGYTTTILGRRRPIPELQSGDPSQRGVGERMAVNSPIQ
                     GSAADLIKLAMIKVHRRLRDELPRCKMILQVHDELIFEVPDQELEQAKQLVKAEMEAT
                     GDALGLSVPLKVDVGVGCNWRAAHP"
     gene            5230..5595
                     /locus_tag="PLS35_11010"
     CDS             5230..5595
                     /locus_tag="PLS35_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNI19240.1"
                     /translation="MTIPEPEFQALVQQALDGLPDEYAKRITNVAVVVEDEPSPEVLA
                     DLDMDEDEDLLGLYQGLPIDKESFFQAGGQLPAKISIYRGPILRLCRTKQEVVQEVRD
                     TVVHEIGHHFGFDDDEMPY"
     gene            5686..6525
                     /locus_tag="PLS35_11015"
     CDS             5686..6525
                     /locus_tag="PLS35_11015"
                     /inference="COORDINATES: protein motif:HMM:NF037404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiV family abortive infection protein"
                     /protein_id="HNI19241.1"
                     /translation="MTAAKTFRLAAVAALTNANQLCAEARLLAENEHNSRAAALAVIG
                     LEEFAKAVACTVAAIFPEKSSDLGNRLNAHNVKQWIYQTFEGIQIQVQDWLNVVPYES
                     GAGSPIEPETILLETFRTLSKSRWGSVVPTKNSADSAAKQDQKYLNDPRMHARPTEIM
                     TTPFIKNVAFYVDVKKGEVLLPNRVDRYANYEIHGLTWSLEHSSPLKQILSDDHKWEA
                     FADAVRRTPGAGLSIEAEVLTPKKTAIKQLRRLPETATWDQIKKEIERGAQEATEERK
                     LTS"
     gene            complement(6546..7799)
                     /locus_tag="PLS35_11020"
     CDS             complement(6546..7799)
                     /locus_tag="PLS35_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19242.1"
                     /translation="MPIENNFQHDELSRKNPGDRLTYADWTPPPDMDSPAWQAAMGEA
                     GQKLSQAGVRVIVFLHGAIPGTDIFGLGRLDEVGGLKRGYSRGISGLDSLLSFMREGT
                     NGITPLPGGMKPPLANDEATKSLLDSQIGDAGNFTAPIVERCRKALNQHVSKPIVCVR
                     ELWSSEHHHLGRALAACRLLDRLRALVTEHHLGTGDRILIQAHGQSGLVLALAANLLC
                     PSPITGRKAFFDLLLAANPQHSDVQAIQRIDPLLTSGSLLNGAALDIVTFGTPVRYGW
                     DPSGIGKLLHIVNHRNLRTDGKTWLSKMELPQISVEMPIAWGGDYVQELAVAGSDALP
                     AESAKAANKAVWELLEPYDGFERWVECARRATRFPSEGQCLLVDYKDCTSSTNVRDHY
                     YGHAAYTRANAILFNFNQIVGRLYS"
     gene            complement(7869..8414)
                     /locus_tag="PLS35_11025"
     CDS             complement(7869..8414)
                     /locus_tag="PLS35_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HNI19243.1"
                     /translation="MPLTSSHILMAVGMAGTLLVATATMAGELTPQDVRTHADQHFQQ
                     LRAREQTIAPTTGDRPQQDPSSSYHPDQFFIGQGQGDLSKGKLVCQRVSELAARADIA
                     KQIRVQVKEHATDRLRERTGREPEQDIEVVREEIVQEYLQGVTIVDRRIDDEAKTCTA
                     VAVMPKATLNTSLPAESSARH"
     gene            complement(8417..9514)
                     /locus_tag="PLS35_11030"
     CDS             complement(8417..9514)
                     /locus_tag="PLS35_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19244.1"
                     /translation="MTDWETRVDAIRLAAEQAKQQALEQVASYLESVTVVQNLDVTQD
                     ELRSYTAGMVLVLDQQVDTALDGDVVVIRVTLKAQVDSEEAAQAILAMKQNEDVRQEL
                     LALKEDVESLHKQLEDANQALAHANSPEQVRKLNQQRTDLLNHAESDAMVAQAWTDWL
                     LIAPFAHPYPSAGVAQVQALLGIAGRLNPTNPHLAIAQQTVGAKLPPAPPHPRQSPVP
                     HTVPFLPGYYVVPKEPAPPGTPPPTDSKQLSSVYQLNPLLPNPSGQPPQSGNSTTIIQ
                     AHPRPGTSPSVPDRNMMPTYRQIPAPNSQASHPTLHTYRAGGSPPSASIPGSADGGIR
                     GGRPPVIHQTKPLSPSESTAERGSATNQGDK"
     gene            9879..10928
                     /locus_tag="PLS35_11035"
     CDS             9879..10928
                     /locus_tag="PLS35_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNI19245.1"
                     /translation="MSFIRNISTVPVAFCLAVVLSMTVVASSSSSVALAARGAGDIPP
                     SVPAGMTEHVILFVLEGFGQESLKSGVMPVLSSLVKEGAVTWSATAVAPARRLPTMAS
                     LVTGMPVAKHGITWNVFEFSRGYPRAPTVFDYLDLSGGRDSAIFYMDESLYQLAKPEP
                     YTDYQMCGPLRAECNPDRLVGYVRDYFKKATSGSGYGHAIPSLPHLLVVHLPAPGRVG
                     EAQGWKSAAYKDALKAVDKAMGTVLDLYRELGLMKRTTVFATSLSAFGETQFSSGDMS
                     ADQAGAVPVVPWIASGVGIKAGYAIRQPVSIIDTGATVMRALGLTTYTEWESHPVEEI
                     FKTAFTVAPVNPLLQ"
     gene            10936..11427
                     /locus_tag="PLS35_11040"
     CDS             10936..11427
                     /locus_tag="PLS35_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19246.1"
                     /translation="MDILSHRLFTGRQTLIRIVALSCAVFAGHAERLMAAGPPPAYTK
                     EHTITIDPTPLVPQSWWQVPGITPPLWVLDPETSDAYRTTETRELHLKPGQYKFISFT
                     FDFPFTVSLDGKLEYSKSLDQCVEGRGTQTLVVRCKRTYPHGGQRDAYYGNSEVGDGT
                     GTR"
     gene            11408..12778
                     /locus_tag="PLS35_11045"
     CDS             11408..12778
                     /locus_tag="PLS35_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19247.1"
                     /translation="MAQALDDLLDSLEDPDDATREEAAKALAQLANPTTLDALIGACG
                     DEYWSVRTRAGWGVAKIGGPKAIEALIALFNDPIMEVRNEAVAAVVSLGVGHLDRLLV
                     AMKDERWRVREHAAKACGDLRDARAVDALIFACRDRDGAVKSAAAEALGKIGDAKAIP
                     ALIKLFRDSSKIVRETAGIALVAIGQPSVDLLLETMKDKDFVVRCHAARALGGMTTDY
                     QIGRSWVREPRVVDALIEALKDPDRAVREDATIALGMIGDPRAIDGLLEAMKDGAVKR
                     HAIASLGMIGDPRALPAVLAALKGKGVRQDGTPTPGCIVSEDAFIKEAAATALGHFRD
                     PRVIPDLIMLLKDGVLREKAAAALVLIGDSAIEPLISFLYDPKASEVEAEGERVLSYA
                     SVRLTAKDSLRLLVVETLEHLGWTPPDEETSVDSSQADNLRVDRPLGDIGRFGPSGDF
                     AKGSVR"
     gene            12959..14041
                     /locus_tag="PLS35_11050"
     CDS             12959..14041
                     /locus_tag="PLS35_11050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19248.1"
                     /translation="MADTVSEQIAALSDEDWAVREEAATLLGSLNDARAVLPLTHALR
                     DTDRAVREAAVGALSSLGSVSVPALAGCLADPALQVQEAASAILASLADARVLAPLMQ
                     ALGSRDWIVRMHAAKGLGRIGDPEAVSALMPLLQDKVKAVREEASTALAAIGVAAMAG
                     LIEALQHDDWLVRLHAVEALGKLKSPDSVEPLLRALFNERDSAIREDVVRALGAIRDG
                     RAVDYLVTVMKEPGLRLLAVEALGHIGDRRVVPILRRVVEGVPLGEPRDSATPCADGW
                     TDEMATMGMAARALGMIADAVAIPSLIVALRNTITRSEAAAALTKFGPAVIPSLLPML
                     AKEQDENVRYHVRETLTAVGWRAGRV"
BASE COUNT         2887 a         3837 c         4418 g         3063 t
ORIGIN      
        1 tggaaattaa ggtcttcaat aacaacgttg aaaaagccct gaaggtcgcc aagaagaagc
       61 tggcgggtga aggattgttc cgcgaactga agcgccgccg gtactatgag aagccgagtg
      121 tccgcaagaa ggcgaaagag cgagaggctc aacgccgccg ccagaagtgg cttgcaaaga
      181 gacgtcccga atagacgcca ggcgtttttc cttccgccag tcatctagac cgtcccaatt
      241 taatccctct ttccggcacc tccatgcagg cgcacgagat tcatgccgtc atccgaacgg
      301 tcaagcggga gattgcccgc tggcctgacc cggttgtcgg cgtcgtggcc aaaaaatccg
      361 gccgcgatcc ctttctggtg ctgatttcct gcctcctcag tcttcgaacc aaagacaaga
      421 ccaccgctga agccagtgcg cggctctttg ccttggcctc aacaccggca acgatgcagc
      481 agctgtccca gtcgacggtt gagtccgcca tctatcccgt gggtttttac cgcaccaaag
      541 ccaagcagat tcagcagatt tgcaggcagc tgcttgatcg ataccacggg cgagtcccgg
      601 atcagattga agcgttactc acgttgccgg gcgtcggtcg aaagaccgcc aacctggtgg
      661 tgacagtcgg gtatgagaaa ccaggcattt gcgtcgacat tcatgtgcat cggatcagca
      721 accggtgggg ctacgtgaaa accaaaacgc ctgatgaaac ggaaaaggcc ttgcgcggga
      781 aattgccacg caagtattgg atcaccttca acgatctgct cgtgccgttc gggcagaacc
      841 tttgccagcc tgtgtctccg ttctgcagcc ggtgcaagat tgcggagtat tgcgatcgag
      901 tcggggtgac aaaaagccgt taatgcagcg tgaccagtcc cggcatcagt cctgtccgga
      961 attggcggcg tcatccgttg atcagatgaa gagtttggtt tccgcctgcg cggtcaaaga
     1021 gagaatcgcg ggaaggccga gcacttcgtc cacgttgcgt tcgaccgcac ccgcttcgac
     1081 gcccatatat tcgagccccg cgctacaggc gatgagacga aattgcccga caagacgggc
     1141 ttcatgaaac atctccgaaa tccggggaac ctttttttcc ttcaagagtc gagcgacctc
     1201 ttcggccgag gcggcatatt ccggaggaaa gtcaatgtgg tcgatcttgc cctcagccag
     1261 cttcttgatg gtccagaaca agagaaccac gatgacatcc ttgcccatag ccgaagccgt
     1321 gaggccgagc gtcgcgacct gatgtagctt gtcgtacgta gcgttatggg cgaagagaac
     1381 aaacttcggc ttatcggcca tggggactat ttgaccttct gtctggtttt gacactgttg
     1441 ataaagtcgc tgatggccgc atctacctca gccgcggtca tggcgcgatc gtgcatttcg
     1501 ctctcatcga gcaggtaacg ctctccctgt ttttcctgcc gtacgaccac agaattttcc
     1561 ggcgtgacct ccacgcgcat gaaccactct ttgttgccct gggtgataac gacttctttg
     1621 ccgagtccga tcttgctgat gtcgatctgt agttctgggg gtgcagcgga atcggcagcc
     1681 tgcgctcctt ggtcgaacac ctcaacggta ttcagcacca gaatcaggcc aaggacggcc
     1741 aggcggcaag ccgaggagcg gatggtcctt agcacgaagc tgctggatcg cggcggcagc
     1801 atggtgcgga attatagctg gcagagcagg gaacgtaaac ccgctgggcg tcggcaccct
     1861 ttccgccgcg taaaggcccg tgctaggatg cgccggtgat gagcgacatg ttggcgcaga
     1921 tcgaatccta cgtgccggcg agcgtgttga tttggtttgc cgtctcgtcc gttgtcatgt
     1981 tcgtgggaac cctgatcgcg attcctgtca ttctgatgcg gttgccggcg gactatttcg
     2041 atacccgcat tcctcgcccc tggatggaga accatcatcc ggtgttgcgg ctgctggggc
     2101 atgtcgtgaa aaatgtcgtc ggggcgattt ttctgttcgc gggattcctc atgctcttct
     2161 tgcccggaca gggcgtgctg accatgttga tcggcctgtc attgatcgag tttcccggca
     2221 agcggcgtgt ggaagccaaa atcgtcggac aatctaccgt tctcagcacg atcaacgcga
     2281 tgcgagccaa gttcgacaaa ccgccgctga tcattgctcc cgagtgacag ccgtcgacgt
     2341 agacatccgt cgcgccagtt atccataacc cgtgtgccta ttcctccatc atgcccactc
     2401 tctatctgat tgacggcagt gcctatatct atcgggcgtt ctttgccctt ccgcccttga
     2461 gcaattccaa gggcttgcag accaatgcgg tctatggctt caccacgatg ttgatgaaag
     2521 tgctgcgtga ccatcatccc gattatgtcg ccgtcgtctt cgatgaaaaa ggcccgactc
     2581 accggcatga ggcctttaaa gaatacaagg cgcaacggcc gcccatgcct caggggatga
     2641 gcgcgcaggt cccgtatatt catcgcgtgg tggaagcgct gtctctgccg gtgattcggc
     2701 aggccgggta tgaggcggac gacctgatcg gcaccctggc acgaaaggga gagggcgaag
     2761 gcctcgatgt cgtgatcgtc accagcgaca aggatatgtt tcagctgctg actcccaaaa
     2821 cgcgcattta cgatcccgtg aaagacaagt ggttcggcga ggcggattca caggtgcggt
     2881 tcggcgtgga gccggcgcgt gtcgtcgaaa ttatgggtct catgggggat accagcgaca
     2941 atattcccgg tgtcaaaggg atcggcgaga aaaccgccgt gaaattgatc acccaattcg
     3001 gcacgatcga ggagctgttg cgccgcgtcg aagaagtgac tccggccaaa acgaagaatc
     3061 tgttgctgga acagggtgag agcgcccgca tgagcaggca gctggcgacc atccaggtgg
     3121 attgtcccat cgaatttgcc cccgctcatt ttcaggcgag agcgccgcag actgaagtgc
     3181 tggtgagcct gctgcgcgaa ctggaattca tgacgttggc caaggctttt cagggcgaca
     3241 ctcctgagca gaaccggtta ggggctgagg ttcagcgcat acacgatact gccggagcgg
     3301 aagatttttt gaaacggcga caaccggaca ccatgatggg gctggcttgt gtattgagtg
     3361 atgaagccgg cgtgcgagcc gacattcaag gctgtgcctt cggctggccg gacgggactg
     3421 ccgcgttcgt acatggggag gcgcaagact ggccgcagcc cctcaccgcg tatgtgcggg
     3481 acgggagcag gccgaaggcg gtgcaggatc tcaaaccgac tctgttagcg ctccagcggc
     3541 aggggatcga catgccaggt ccctactttg acacgatggt tgcagattat ttgctcaatc
     3601 ccaatcgacg ggcgcatacc ttggaagcga tcgccatgga cctactgagt tatcagctcg
     3661 gcacgggcga acgcgaggaa gggggcaagg aaccgcagtc tctgtttgat gtcgatgaac
     3721 gtgtggttcg tcgaaccggc gaggcggcgg cggtcacggc caaagttgcg cccatgttgc
     3781 gcgatcggct gaaagagcag ggcagtctgt cgctttttga acaagtggaa atgccactgg
     3841 tgccggtgtt agccgacatc gagcgcaacg ggtttttgct cgatgtcgag gggctgcgca
     3901 ccttgagcaa ggagttggag cgggaactcg agcagatggt cgggaccatt tatcggttgg
     3961 gcggcggcga attcaatatc ggctcgccga agcagttggc cacggtgcta ttcgaaacgc
     4021 tgggcctgaa gccgctacgg aaaacgaaga cggggttttc taccgatgaa gacacgctca
     4081 cgcaactcgc gaatcagcac gagcttcccg cgcaaattct cagctatcgc acgctcacca
     4141 aactcaaatc gacctatgtc gatgcattgc cgcagctggt caatcctgat acgaagcggc
     4201 ttcatacgtc gctcaatcag acggtcgccg ctacgggacg actctcgtcg accgatccca
     4261 atctgcaaaa cattccggtg aaaggcgact acggcctgcg gatccgtgag gcctttattg
     4321 cgccgcccgg ccatcacctg ctctgtgccg actatagtca ggtcgagccg cgcattctgg
     4381 cccacctctc ccaggatcct cgattattgg aagtgttcga gaagggggaa gacattcaca
     4441 tggcgacagc catggaaatt ttcaacctgc ctgccggcga cgtcacgcgc gaaatgcggc
     4501 gcgcggcgaa aagcgtcgtg ttcgggatcg tctatggcat cagtccgttc ggcctcgcat
     4561 cgaacatcgg tgtgccacaa gctgaggcca aaaaatacat cgaaaccttc tttgaaaagt
     4621 ttgcagcggt gcgtgctctg atggaccaca acatcaacga tgggaaaacc aagggctaca
     4681 cgaccaccat tctgggccgt cgtcgtccca ttcccgaatt gcagagcggt gatccatctc
     4741 aacgcggggt gggcgagcgc atggcggtca atagtccgat tcaaggctcg gcggcggatc
     4801 tgattaagct ggccatgatc aaggtgcatc ggcgcttgcg agacgaactg ccgcgctgca
     4861 aaatgatcct ccaggttcat gacgaactga tcttcgaagt gccggatcag gagctggagc
     4921 aggcgaagca actcgtcaaa gcggagatgg aagcgaccgg tgacgcgttg gggttatcgg
     4981 tgccgctcaa agtggatgtc ggagttgggt gtaactggcg cgccgcccat ccgtagccgg
     5041 atgttgaaac aggttgccag cggccttctc agtcgcacgg cttcctgcga catctcccag
     5101 gctgtatgac tcagtcgccg cactccatgc ggccttgccg gacagcctgt ttgaacatcc
     5161 tgaacaaagc agctgttgtg tagagcgtaa gttggttcag ttcagcccat gtcgccgcga
     5221 aagcgtagat tgaccatccc agagccggag tttcaggcct tggttcagca ggcgctggac
     5281 ggccttcctg acgaatacgc caagcgcatt accaacgtcg ccgtggtggt agaggatgag
     5341 ccttcccccg aggtcctcgc cgatcttgac atggacgagg acgaggatct gctcggcctc
     5401 tatcaaggcc ttcccatcga taaagagtct ttctttcagg cgggcgggca actgccggcc
     5461 aaaatctcca tctatcgcgg accgatcctc agactgtgtc gcacgaaaca ggaagtggtg
     5521 caggaagtgc gtgatacggt cgtgcacgag atcggtcatc atttcgggtt tgacgacgac
     5581 gagatgccat attagccgga tactgaaaca ggctgccagc ggcgttctct gtcacgactc
     5641 tctgcgatgt ccacagccgc atgtctcggg agacatattc acgaaatgac agctgctaag
     5701 accttccgat tggctgcagt cgcggcgctg acgaatgcca atcaattgtg tgccgaagcg
     5761 agacttctgg ccgaaaacga acataattca agggcggcag ccctagcggt tataggtttg
     5821 gaagaatttg ctaaggcggt ggcctgtacg gtcgcggcaa tctttcctga gaagagcagt
     5881 gatctaggga accgtcttaa tgcccataat gtgaagcaat ggatttacca gacgtttgaa
     5941 ggcatacaaa ttcaggttca agattggctc aacgtagttc cttacgaatc gggcgccggt
     6001 tctcccatag agccagaaac aattcttttg gagactttta gaacgctttc gaaaagcaga
     6061 tggggcagtg tcgttcccac taagaattct gccgattccg ctgcaaagca ggatcaaaag
     6121 tatttaaacg atccgagaat gcatgctcgt cccaccgaga tcatgacaac cccattcata
     6181 aagaacgtcg cattttatgt agatgttaag aagggagagg ttctcctgcc taacagggtc
     6241 gatagatacg ccaattatga gattcacggt cttacttggt cattagaaca cagttctcct
     6301 ctgaagcaga tcctttcaga tgatcataaa tgggaggcct ttgcagatgc tgttcggcga
     6361 acaccaggag ctggcctgag catagaagca gaggtactga cgccaaagaa aacggcgatt
     6421 aagcagctcc ggcgattgcc cgagacggcg acatgggatc aaatcaagaa ggaaattgag
     6481 aggggcgccc aggaggccac ggaagaacga aagctgactt cgtagcggga tgtgttaggt
     6541 cacgcttacg agtacagccg cccgacgatc tgattgaagt tgaagagaat cgcgtttgcg
     6601 cgcgtgtagg cggcgtggcc gtagtaatgg tcgcggacgt tggttgagct cgtgcagtct
     6661 ttgtaatcga cgagcaggca ctgtccttcg ctgggaaagc gggtagcgcg gcgcgcgcat
     6721 tccacccaac gttcaaaccc atcgtaaggt tcgagcaatt cccacacggc tttattggct
     6781 gctttggcac tctcagcggg tagggcatcg ctgccggcca cagccaattc ctgcacgtag
     6841 tcgccgcccc acgcaatcgg catttcgacg gagatctgcg ggagctccat tttggagagc
     6901 caggtctttc catcggtgcg cagattgcgg tgattgacga tatgtagaag cttgccgatg
     6961 ccggaggggt cccatccgta ccgcactggt gtgccgaagg tgacgatgtc gagggccgcg
     7021 ccgttgagga gggagcccga agtcaacaaa ggatcgatgc gctgaatggc ctgcacatcc
     7081 gaatgctgcg ggttcgccgc caggagcaga tcgaaaaacg ctttccggcc ggtaatgggc
     7141 gaagggcaga gtagattcgc ggcgagcgcg agcacaagtc cggattgccc atgggcttga
     7201 atcagaatgc gatccccggt tcccagatgg tgttcggtca ctaaggcccg cagacgatcc
     7261 aagagccggc aggccgccag ggcacggccg agatggtgat gctcgctgga ccagagttcg
     7321 cgtacgcaga cgatgggttt ggacacgtgc tgattgaggg ccttgcggca ccgttccacg
     7381 atcggtgcag tgaaattgcc tgcgtcgcca atctggctat cgagcaggct tttggtcgcc
     7441 tcgtcattgg cgagcggcgg tttcatgccg cctggcagcg gtgtgatgcc gttggtgcct
     7501 tctcgcataa acgacaggag cgaatccaat ccggagatgc cgcgtgaata tccgcgtttg
     7561 aggccgccca cttcgtcgag tcgtcctaaa ccgaagatgt cggtgccggg aatagcccca
     7621 tgcagaaaga cgattactcg cactccggcc tgcgacagct tctggccggc ctcgcccata
     7681 gcggcttgcc aggcgggcga atccatgtca ggcggcggcg tccagtccgc ataggtgaga
     7741 cgatcgccag gatttttcct ggagagttcg tcatgctgga agttgttttc gatcggcatg
     7801 ggaagattgt cttacccgaa acgcagtccg aagttcaatg gagggggatg ttcggaaggt
     7861 ggggacgctc agtgacgggc ggacgattcg gccggaagtg atgtattgag ggtggctttc
     7921 ggcataacgg ccaccgccgt acacgtcttt gcttcgtcgt cgatgcggcg atcgacgatc
     7981 gtcacgccct gcagatattc ttgaacgatt tcttcgcgaa ccacttcgat atcctgctct
     8041 ggttcacgac cggtgcgttc cctcaggcgg tcggtggcat gctccttgac ctggacccga
     8101 atttgtttgg cgatgtcagc ccgagcggcc aattcggata ctcgctgaca caccagtttt
     8161 cctttactca aatcgccctg gccctgtccg atgaaaaatt ggtcgggatg gtaggaggac
     8221 gaagggtcct gctgcggccg gtctccggtt gttggtgcaa tagtctgttc tcgtgcgcga
     8281 agctgctgaa agtgttggtc ggcatgcgtc cgaacgtctt ggggtgtgag ttcccctgcc
     8341 atggtggctg tggccacaag cagagtcccg gccatcccta ctgccatgag gatgtgactg
     8401 gatgtcagtg gcatggctat ttgtcccctt gattggtggc ggaaccgcgt tccgcagtgg
     8461 actcggaagg cgaaagcggt ttcgtttggt ggatgaccgg ggggcgccct cctcgaatgc
     8521 caccgtcagc cgatcctggt atcgacgccg aggggggact gcctccggcg cgataggtat
     8581 gcaaagtcgg atgacttgcc tgactattcg gcgccgggat ctgtcgatag gtcggcatca
     8641 tgttgcggtc cgggacggac ggtgaggtgc cgggtcgggg atgagcctgg ataatcgtcg
     8701 tactgttgcc ggattgtggc ggttgcccgg atggattggg caacaacgga ttgagttggt
     8761 agacgcttga gagctgcttg gaatcagtgg gtggaggtgt gcctggggga gccggttcct
     8821 tcggcacgac atagtacccc ggcaaaaatg gcacggtatg cggcacgggt gactgtcgag
     8881 ggtgaggggg tgccgggggg agcttggctc ctacggtctg ttgtgcaatg gcgaggtgag
     8941 gattagtcgg gttcaaccgc cctgcgatgc caagcagcgc ttggacctga gccacgcctg
     9001 ctgaagggta ggggtgcgcg aagggggcaa tcaacagcca gtcggtccag gcctgcgcca
     9061 ccatggcatc cgattcggcg tgattgagca gatccgtacg ctgctggttg agttttctga
     9121 cctgctcagg tgaattcgcg tgggcgagcg cctgattggc gtcttcgagt tgtttatgca
     9181 ggctctcgac atcttccttg agggcgagta attcctgccg cacgtcctcg ttctgcttca
     9241 tcgcgagaat ggcctgcgcc gcctcttcgc tgtctacctg ggctttgagg gtgacgcgga
     9301 tcaccacgac atctccatca agagcggtgt ccacctgttg atccagcacc aggaccatcc
     9361 cggctgtgta gctgcgaagt tcatcttgag tcacgtcgag attctgcacc acggtgacac
     9421 tttccagata tgacgcgacc tgttccagcg cctgttgttt cgcctgctcc gcggcgagcc
     9481 ggatcgcatc gaccctggtt tcccagtctg tcatgcgatg ctgtcctgtg gccgtcacca
     9541 cgcgaacgtc ggcaagactt gggctaggag aggcgaagaa gaggagaaag cctgctgtga
     9601 aaagagcgac gagcggcatg aggcgacgct tccctgaaca ctcgacggtc aggcagaaga
     9661 aatcactgga agaacggttg ccgggcattg tggtgcaccg ctgtaaaatg ggtgttcgga
     9721 gggttgatcg cactggcctc ttcccgattc gaccaggata ccatcgaacc cgctcaggcg
     9781 ctatgagagc cgaggttcgt gccttgcctt cccggaaaac agcgtgctaa ggtacttttt
     9841 cttcgcttca taaatcgatc gtctggtgag gcaatctcat gagttttatc cgcaatattt
     9901 ccaccgtccc ggttgcgttt tgcctggcgg ttgttctgag tatgaccgtc gtggcctcgt
     9961 cctcttcctc ggtggccttg gctgcacggg gtgcaggaga cattccgcct tcggtccccg
    10021 cagggatgac agaacatgtc atcctgtttg tcctggaagg gtttggccag gaatcattga
    10081 agagcggtgt gatgccggtc ttgtcttctc tcgtgaagga gggggccgtt acctggtcgg
    10141 cgacggcggt tgcgcctgcg cgccggttac caaccatggc atccttggtg acggggatgc
    10201 cggtcgccaa acatggcatc acctggaacg tctttgaatt cagccgggga tacccgcgcg
    10261 ctccgacggt gtttgattat ctcgatttga gcggcggccg ggacagtgcc attttctaca
    10321 tggacgaatc tctgtatcag ctcgccaaac cggaacccta tacggactat cagatgtgcg
    10381 ggccactcag agccgagtgc aatcccgacc ggttggtggg gtatgtgcgt gactacttca
    10441 agaaggccac aagcggttcc ggatatggcc atgccattcc gtccttgccg cacctgctgg
    10501 tcgtgcatct gccggctccc ggtcgtgtcg gcgaagcgca gggctggaaa tcagccgcat
    10561 acaaagacgc attgaaagct gtcgacaagg cgatgggcac ggtgttggat ctctaccgtg
    10621 aactcggctt gatgaaacgg acgacggtct ttgcgacgtc gctgagcgcc tttggagaga
    10681 cgcaattttc atccggtgac atgtccgccg atcaagcagg cgccgtcccg gtcgtgccct
    10741 ggattgcctc aggcgtcggc atcaaggccg gttacgcgat tcgtcagcca gtgtcgatta
    10801 tcgatacggg cgcgactgtc atgcgcgcgc ttggactgac gacctacacc gaatgggaga
    10861 gtcacccggt cgaggaaatt tttaagaccg cgttcacggt tgctcctgtc aatccactcc
    10921 ttcaatagag gaccaatgga tatcttgtcg cacagattgt ttacggggcg gcagacgttg
    10981 atccggatcg tcgcattgtc atgcgctgtg ttcgccggac atgcggagcg gcttatggca
    11041 gccggtccgc cgcctgccta taccaaggaa catacgatta caatcgatcc gactccgttg
    11101 gtgccgcaat cctggtggca ggtgcccggc atcacacccc cgctctgggt attggatcca
    11161 gaaacgtcgg atgcgtaccg gacgaccgag actcgcgaat tgcacctcaa gccgggacaa
    11221 tataagttca tcagttttac cttcgacttt cccttcacgg tgagtttgga cgggaaactg
    11281 gaatactcca agtcactcga tcaatgtgtg gagggccgcg ggactcagac cctggtggta
    11341 cggtgcaagc gcacctatcc tcatggcggt caacgtgatg cctattatgg gaatagtgag
    11401 gtgggtgatg gcacaggcac tcgatgatct actggattcc ctcgaagatc cggatgatgc
    11461 aactcgtgaa gaagcggcca aggccttggc gcagttggcg aacccgacca cgctggacgc
    11521 gctgatcggc gcctgcggcg acgagtattg gtctgtgcgt acgcgcgccg gctggggtgt
    11581 cgccaaaatc ggcggcccga aggcgatcga agcgctgatt gcactcttca acgatcccat
    11641 catggaagtt cgcaacgaag cggtcgcggc cgtggtgtcc ctgggcgtgg ggcacctcga
    11701 tcggcttctt gtggcgatga aggacgaacg gtggcgtgtc agggaacatg cggcgaaagc
    11761 ctgtggcgat cttcgcgatg cacgcgcggt tgacgccttg atctttgcgt gtcgggatcg
    11821 cgacggtgct gtgaaaagcg cggcggcgga agcgttggga aaaattggcg acgccaaagc
    11881 cattcctgcc ttgatcaagt tgttccggga ttcctcgaaa attgtgcgtg agaccgccgg
    11941 catcgcgctg gtcgcaattg gacagccatc cgtcgacctg ctgcttgaaa cgatgaagga
    12001 caaggatttt gtggtgcgtt gccatgcagc ccgggcattg ggtgggatga cgacggacta
    12061 tcaaatcggc cggagttggg tgcgtgaacc gcgcgttgtt gatgcgctga tcgaagccct
    12121 gaaagatccg gaccgggccg tgcgcgaaga tgccacgatt gccttgggca tgatcggtga
    12181 cccccgcgcg atcgatgggt tgctcgaagc catgaaagat ggcgccgtga agcgtcatgc
    12241 catcgcgtcg ctcggcatga tcggtgaccc gcgggctctt ccggcggtgc tggcggcgct
    12301 caagggcaaa ggtgttcgtc aggatgggac gcccaccccg gggtgtatcg tcagtgaaga
    12361 cgcgtttatt aaggaagcag ctgcgaccgc acttggacat ttccgcgatc cccgtgtgat
    12421 ccccgatctc atcatgctgt tgaaggatgg cgtgttgcgc gaaaaggctg ccgcagcgtt
    12481 ggtgttgatc ggcgattcgg ccatcgagcc gctgatttca ttcctgtacg atccgaaggc
    12541 ctccgaagtt gaagccgaag gcgagcgggt gctgtcttac gcgtcggtgc ggttgaccgc
    12601 gaaggattcc ttgcggttgc tggtcgtgga aacgctggag cacctgggat ggacacctcc
    12661 ggatgaagaa acctcggtgg attccagcca ggccgacaat cttcgtgtcg atcggccgtt
    12721 gggagatatc ggtcgcttcg gtccgagcgg agattttgcg aaaggatcgg tgcggtaatt
    12781 ctcggcgttc tctcgcgggg tgcctggcct gttgagagct tccggcgtcc ccgcgtctct
    12841 tcctctccgt ttcgaccagc gtgcgggatg gtgcgatgtc aggtcgtagg gccaacccgc
    12901 gttgacccct aaaaaaatgc tgtgttaagg tcgatggcct cccagaggcc gaggtaccat
    12961 ggccgacacc gtcagtgaac agatcgcagc cctgtcggat gaggattggg ccgttcgcga
    13021 ggaagcggcg accctgctgg ggagcttaaa cgatgcgcgg gccgtactgc ctttgactca
    13081 tgcgctgcgc gataccgatc gtgctgtccg cgaagcggca gtgggtgccc tctcatcact
    13141 cggatccgtt tctgttccgg ccttggcagg ttgtttggct gatccggcct tgcaggtgca
    13201 agaagcggcc tcggccattc tggcttcact ggccgatgcg cgcgtcctgg cgcctctcat
    13261 gcaggccttg ggcagtcgtg attggatcgt gcgcatgcac gccgcaaagg gcctggggcg
    13321 gatcggcgat ccggaagcag tgtccgcgtt gatgccgctg ctgcaggaca aggtcaaggc
    13381 tgttcgcgag gaagcatcca ctgcgctggc ggccatcggc gtggccgcga tggcgggatt
    13441 gatcgaagcc ctgcagcatg atgattggct ggttcgcctt catgcggtgg aggcgttggg
    13501 aaagctcaag tcgccggact ccgtcgagcc cttgctgagg gccttattca atgaaaggga
    13561 ctcagcgatt cgcgaagatg tggtccgggc gctgggtgcg atacgagacg ggcgtgcggt
    13621 agattatttg gtaacggtga tgaaggagcc gggattacgg ctcttggccg tcgaggccct
    13681 tggtcatatc ggcgaccgcc gggtcgtgcc gattttgcgg cgggttgtcg agggcgtccc
    13741 gctgggtgag ccgagggatt cggccacacc ctgtgccgac ggatggacgg atgagatggc
    13801 gaccatggga atggcggccc gtgcattagg gatgattgcc gatgccgtgg cgattccatc
    13861 actcatcgta gccttgcgga ataccatcac caggtcggaa gccgcggcgg ccctgacgaa
    13921 attcggtccg gcggtgattc cgtctctctt accgatgttg gccaaagagc aggatgagaa
    13981 tgtccggtat cacgttcggg aaacacttac ggcggtcggg tggcgcgccg ggcgagtctg
    14041 aacgtaacgc tgtgcggatc gtgtgagaac gctatttcgg aagcaggctt catcgtaagc
    14101 aatcagtaaa acatcggcta cgcaactttt tcacgatgtc caaagagtcc atcgatacct
    14161 tagtttccga gttgacgcat gaggaggagt ggcggcgtat gcgag
//
LOCUS       DAOQEW010000093        14128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1215_length_14128_cov_8.351951, whole genome shotgun sequence.
ACCESSION   DAOQEW010000093 DAOQEW010000000
VERSION     DAOQEW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_14128_cov_8.351951"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLS35_11055"
     CDS             complement(<1..712)
                     /locus_tag="PLS35_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19249.1"
                     /translation="MRRTWVGLAAVFMLAMVIGVMPAVVRADCVDGVRDPTSEELAFA
                     ARAEAALAAALPSPIANSERRGGTYDFSRQPRLSFCTSNQVGAFIPNVSGGYLYKFPK
                     AEADRLYAERKAVEKQIEEAEKLPPEKDAEYKQVLAQMKAAYDAAPRRSRKDPPFTPE
                     QQEQVTRAMAEGKKLEDAAKKLLSDHVASVKSQTDPLRAQAKRLESYPQDLAVRLAMN
                     MERFPEPGPTIATFGAPSA"
     gene            858..2084
                     /locus_tag="PLS35_11060"
     CDS             858..2084
                     /locus_tag="PLS35_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI19250.1"
                     /translation="MTTQASPSSDDQSDTPTPHRSPDPAPDGPRPAATELDRFFELSL
                     DMLCIANADSYFTRLSPAFTRTLGWSLEELMSRPFLDFVHPDDRAMTLLEVERQIGRG
                     EPVLEFQNRYQHKNGSWRLLSWKSSPHPDGRMYAIARDVTDREQAQQALRRSHEELEH
                     RVQERTAELQQRNRDLETLLYVSSHDLREPLRSISNFSQLVRDRYGDRLDAKGLDYID
                     RVVRAAQRMDQLMEDLLTLSRAQRLEMPYEDVPGEDVVKAALTQLDETIRRTGARVQV
                     AANFPWFRVNKTWITQALYNLIANALKFHLAGKVPEIEIAPFYLTEETVPLAGIVVRD
                     RGIGVEPDQAQRIFQLFQRAVGREVSGTGAGLAIVQQIAHRHGGRAWVQPREGGGSEF
                     VLLFGSQSVIGKKPAS"
     gene            complement(2293..3468)
                     /locus_tag="PLS35_11065"
     CDS             complement(2293..3468)
                     /locus_tag="PLS35_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNI19251.1"
                     /translation="MKLYRSFILTLGLLLACNTLASAEILALLNYESKPDQTVRREGI
                     AIMDIDPESGNFGKILMEIPLPPDLVAHHIFFNRDRSKAYVTALGKSILHVVNLTEFP
                     YRLRAIDVPDCQMGEDLAVSEDNRTWYLTCMGSNNVIMGDALLDKPIKTVSAAEPALA
                     TIQYPHGIAIHNGIDRVLVTSTVKPDMSDAGDSITVMEAGSGRVLSTHKVSAKPSPAK
                     AAPVEVMFSPNSNPPAVHITNMLEGTLWAGLWDPASKTFSFHQVDDFGPRQQGMPLEM
                     LYNAKGDRLFVTTAKPGFVNLYDNSDPGQPKFLKTIAAAAGAHHSVLSPDERYLFVQN
                     SFLNLDGMSDGSITVIDLKADTILGNIDTLKAQGFNPNCIMLLPAQPGDLRASRVTE"
     gene            complement(3599..3949)
                     /locus_tag="PLS35_11070"
     CDS             complement(3599..3949)
                     /locus_tag="PLS35_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNI19252.1"
                     /translation="MTNATTTEIDQTTGLYDMTNLGRVKDLGAHAPEAMKAFVAFDKA
                     ALAPGAIPGKYKELMALAVAFTTQCPYCIELHANKAKEYGASAAEIAESVLVTAALRA
                     GGAVTHGTHALKGI"
     gene            complement(3981..4802)
                     /locus_tag="PLS35_11075"
     CDS             complement(3981..4802)
                     /locus_tag="PLS35_11075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI19253.1"
                     /translation="MTTNTIMPELDSLKTRLNTIWTAGDYDRFSRYMEGGAREFYERL
                     QIAPGCRLLDVGCGSGQLALIAAKDGLEVTGVDIAANWVERAQARARAEGVSARFEVA
                     DAEALPYETASFDAVTSLIGAMFAPRPDLVAQELLRVCTPGGTLAMANWTAEGFVGQM
                     FKNVAKFIGPSGMPSPVLWGNEATVQERLGHGLSTISLTRRQYLFSYPFPPSEVVEFF
                     RLYYGPTNRAFASLEAEGQAHLRRDLETLWAAHNQAGPDCTTVYGEYLEVIGIRS"
     gene            4998..6017
                     /locus_tag="PLS35_11080"
     CDS             4998..6017
                     /locus_tag="PLS35_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNI19254.1"
                     /translation="MDVLSEVLKAVKLDGAVFFHGEFAAPWCAQEPDAETMASYLPTQ
                     AGHIIIFHLLLEGRGYIRLGDEDRAVPLTAGDIIILTQGDAHYMGNGPPVTPVDTSLQ
                     IRQILAEGRMMSQLAGGGEITKLICGYLTCDAQLCRVVLAGLPAMLKVNIRDTPSGHW
                     LENTLRYSVDHAEASGPGGAAVIAKLSEALFVETLRRYIAQLPHTETGWLAGVRDPDV
                     GKALALLHQRPAQPWTIATLAAEVGVSRSVLAERFQHFLADTPIGYLTRWRLHLAAQL
                     LTSTSKSVAEVAGDIGYESEPSFNRAFKREFNLPPARFRAQSKASLAGNGAVSRQDHR
                     SAPAR"
     gene            complement(5993..6331)
                     /locus_tag="PLS35_11085"
     CDS             complement(5993..6331)
                     /locus_tag="PLS35_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19255.1"
                     /translation="MKTIVLCVCMSLLLPVMSEARCRTIGESASLGSEYGTFPLYPAE
                     QVVEAGQKRKSIAMQWLGYGIGVPLFVVAMPFAMVGAVAGAAAHPWTKCKAMEGRFEG
                     PEVHLAGADR"
     gene            6820..7302
                     /locus_tag="PLS35_11090"
     CDS             6820..7302
                     /locus_tag="PLS35_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19256.1"
                     /translation="MLFHTSASGIEQPSALQADTALSARLLVSWLVFWILALPLMHVH
                     IQAERHQGLPHNVLAGDLPGEFVSSADSAIEAAQPAGVPPPPHLASGISYPELAFTAP
                     VPSKHHWLFSLTAIFTRDIPAEMFPTGRTVTPDQRSFDHPTPLPLSSPRAPPPVDRLT
                     "
     gene            7415..7684
                     /locus_tag="PLS35_11095"
     CDS             7415..7684
                     /locus_tag="PLS35_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19257.1"
                     /translation="MKLFALAVVSSTTLLLGSTGTAFSAGASSDSNSVKEASPSAQLI
                     LAKQGRGGHDDPPGDDHDGVVLAKQGRGGHDDPPGDDHGDHVIIG"
     assembly_gap    7701..7800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8156..8614
                     /locus_tag="PLS35_11100"
     CDS             8156..8614
                     /locus_tag="PLS35_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI19258.1"
                     /translation="MHPLIVLAFIALAGGLLPIPAATFAQALPPALMQKIQAAQQHVN
                     TIGMEAYRKVTDNPGDALIIDVREPQEYAAGHVPGAVNIPRGVLEFQIWKHVGSPGAP
                     QMAKPLYLQCQSGNRASLAAQSLAELGFSQTTAVVMSLEEWQKAGHPFVK"
     gene            9050..10249
                     /locus_tag="PLS35_11105"
     CDS             9050..10249
                     /locus_tag="PLS35_11105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI19259.1"
                     /translation="MSVIIPTYNRAPLLKTAITSALHQERAGDLFDVEVIVVDDCSPE
                     DMSPIVSEFPGVRYIRLPENRGASGARNAGIKEASGKYVALLDDDDEFLTHKLMIQVP
                     VLEADPDVGVVYGQSVVTGSDTPLLLWPEWGPSGRVFEEFLTRTEDFLHPPTWLVRRE
                     LFDAAGWFNEQHRTMEHYDMALRLSMLTSWTFLSGGPVARGRFSKKGKWYSNIVNGTN
                     EERLPRIVESALARLPATPEAERTRRKARAAVCATIAGQRWWTGGGLEPTKQHLLASL
                     RTSAWLLHEAPVMEWIQRIAGTVAGGSHQPVQAVRAFWEEIKQATSAAPDAHTIPMRG
                     VLGDMLSSAAITMKQGSPRRAWMVGLGSIVHNPKSLKQPARLLHLYRALATTPATSGT
                     RLPISPS"
     gene            complement(10322..11083)
                     /locus_tag="PLS35_11110"
     CDS             complement(10322..11083)
                     /locus_tag="PLS35_11110"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HNI19260.1"
                     /translation="MPDPVQMQFSQANAYLTELISQFAGIEKRIEGGETVTLNLSDQG
                     RTLSLVVSREHVTESAPPAKSPTGGVSSMLADNHSLVAALTKTFVFDLGQANRNISAA
                     DVQRHFTHATRQTRRDEADLIPNVPDPVVKHDGHLWVVAEDCRYVALDGCIITAKRGF
                     KSDLASIPRLFWAVIASFELSLVAPIMHDLIYRCGGQVILPDGEVIPAGKLFSRKDAD
                     DVFLELMTRSKVAYWKRNVAYLAVRAFGQSSWQGR"
     gene            complement(11424..11702)
                     /locus_tag="PLS35_11115"
     CDS             complement(11424..11702)
                     /locus_tag="PLS35_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19261.1"
                     /translation="MANLFARHPHTVKDMIVAETLCPLHQEPVKVGFEMNGVQGKLTV
                     REHCSILQMGEKCSEACRSNPEILEAVHKKMAEFKEESAREIPIISTP"
     gene            11897..12178
                     /locus_tag="PLS35_11120"
     CDS             11897..12178
                     /locus_tag="PLS35_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNI19262.1"
                     /translation="MPYVLIIHEVDAYPAWKSIFDQAAQIRKQAGEIRYQLLQHDTNP
                     NRLVHFSEWTSLDKARSFFESPELVEIRRKAGVKAPAFHYLNEVEQGRL"
     gene            12323..12508
                     /locus_tag="PLS35_11125"
     CDS             12323..12508
                     /locus_tag="PLS35_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19263.1"
                     /translation="MRFVKVKDEERPGEVAINLDLVREAHFGGGLLHLYFERSSSAQD
                     DMTFTGENAQKIWAAMG"
     gene            12677..>14128
                     /locus_tag="PLS35_11130"
     CDS             12677..>14128
                     /locus_tag="PLS35_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNI19264.1"
                     /translation="MPNHRIDLKRLARHAMIDRGLWPDFSAKAMAELAQIRAAAVDHG
                     ADAQDLRDLLWVSIDNDDSLDLDQITVATEHPDHSVTLLVAIADVDALVPKDSAIDTH
                     ARHNTTSVYTSGDIFPMLPEPLSHDLTSLREHQDRLAVVVDLAIAANGKVSGASHYRA
                     LVRNHAKLAYNSVAAWLTGTGPAPDSISDVPGLDAQLRLQDRVAQQLKARRHQQGALS
                     LETIEPRAVFDGDTLTGITVEQKNRAKELIEDCMIAANQATASYLKRKGIPSFRRILR
                     SPERWQRIVDVAASCGESLPPQPDAQALDAFLVKRRAADPIRFPDLSLAIVKLIGRGE
                     YVLDEGTGGAPEHFGLAVKGYTHSTAPNRRYPDLVTQRLLKAALAGAPLPYRTDELKY
                     LADYCTEKEDDAERVERQMRKSAAALLLQPRIGERFEAIVTGLSEKGTWVRLLEPPVE
                     GKLSGGVMGLDVGDRLTVELVSTNVERGFIDFSR"
BASE COUNT         2890 a         4342 c         3873 g         2923 t
ORIGIN      
        1 gggcgctcgg cgcgccgaat gtcgctatcg tagggcctgg ctcagggaat cgttccatgt
       61 tcatcgcgag gcgcaccgcc agatcctgcg gataggattc caaccgctta gcttgggcgc
      121 gcagtgggtc ggtttgagat ttgaccgacg cgacatggtc gctgagcagc ttcttcgccg
      181 cgtcttcgag tttctttccc tcagccatgg cacgagtcac ttgctcctgt tgctcgggcg
      241 tgaagggtgg gtccttccgg ctgcgacgtg gcgcggcatc gtaggcagcc ttcatctgcg
      301 cgagtacctg cttatattcc gcgtccttct ccggcggcaa tttctccgcc tcttcaatct
      361 gcttctcaac cgccttgcgt tcggcgtaca accgatcggc ctccgccttg ggaaatttgt
      421 acagataccc cccgctcaca ttgggaatga acgcccctac ctggttggat gtacaaaacg
      481 agaggcgcgg ttgccgggaa aaatcatagg ttcccccgcg ccgttcgcta ttggcaatgg
      541 gagatggtaa tgccgccgcc agtgctgctt ccgcgcgagc tgcgaacgcc agttcttccg
      601 atgtcggatc acggaccccg tccacgcaat ccgcgcggac caccgccggc atgacaccaa
      661 tgaccatcgc gagcatgaat accgcagcaa gcccaaccca tgtcctacgc attgcagccc
      721 tcccactctg agtgccaata gcaattccca gccaatacga cctgagtata ggcaatgggt
      781 gaacgaagtc aacgtgtaca aggtcgccgg cgtacagaat tcttgcggga atctacctgt
      841 ttgactaaga tccatccatg acgacacagg cctcgccttc ttctgacgac caatctgaca
      901 caccgacgcc tcatcgctca cccgatcccg cccccgacgg gccacggccg gcagcaacag
      961 agctcgatcg ctttttcgag ctgtctttag acatgctctg cattgcgaac gcggacagct
     1021 atttcacacg cctgagtccc gcatttaccc gcaccctagg ctggagtctc gaagaattga
     1081 tgagccgacc gttccttgat ttcgtccatc ccgacgatcg ggccatgacc ctcctcgagg
     1141 tcgagcgcca gatcggccgg ggtgagccgg tcctggaatt tcaaaaccgc tatcagcaca
     1201 agaacggttc atggcggctg ctgtcgtgga agtcttcgcc gcatccggat ggacgcatgt
     1261 atgcgattgc acgcgacgtc acggaccgcg agcaggcaca gcaggcgtta cgacgctccc
     1321 atgaggaatt ggagcaccgc gttcaggaac ggacggcgga actccagcag cgcaatcgcg
     1381 atttggaaac gctgctctat gtcagctctc acgatctgcg tgagcccctg cgatcaattt
     1441 caaacttctc gcaactggtg cgagaccggt acggagatcg tctggacgcg aagggcctcg
     1501 attacatcga tcgcgtcgtt cgcgcggcgc aacgcatgga tcagctgatg gaggatctcc
     1561 tcacgctgtc gagggcccaa cgattagaga tgccctacga agacgtccct ggcgaagacg
     1621 tggtcaaagc cgccttgacg caactggatg agacgattcg acgcaccggg gcacgtgtcc
     1681 aggtagccgc gaactttccc tggtttcgcg tcaataaaac gtggatcacg caagcgctgt
     1741 acaacctcat tgcgaacgcg ctgaagtttc acctggccgg gaaagtcccc gagattgaga
     1801 tcgccccttt ctatctgacg gaagagacgg tcccgctggc agggattgtg gtgcgcgacc
     1861 gtggtatcgg ggttgagccg gatcaggcgc agcggatctt tcaattgttt caacgggcag
     1921 tgggacgcga agtgagcggc accggcgcgg gcctggccat cgttcagcaa atcgcacatc
     1981 ggcacggcgg gcgcgcgtgg gtgcaaccgc gggaaggcgg cggatcggaa ttcgtgctgc
     2041 tcttcggaag ccaatcagtc atcggcaaga aaccggcatc ctgatttcac cgctcctcag
     2101 ttcgaccgtt aactcaattg cctggggcgg cccggacagc agatgccccc gtaccacatc
     2161 cgaccgatac aagttccgcc ctggagggag cccgctgccg tagaacggca accggctccc
     2221 tcctctctcg agaccgctga aattcacacc ggcttcatcg tatgtggtta actacccaca
     2281 ccacccggtc cttcactcag tgacccgact cgcccgcaag tcgcccggtt gagcgggcag
     2341 cagcatgatg cagtttggat tgaatccctg agccttcagc gtgtcgatat tccccaggat
     2401 tgtatctgcc ttgagatcga tcacggtaat cgagccatcg ctcatcccgt ccaggttgag
     2461 aaagctgttc tggacgaaca ggtagcgctc gtccggcgac agcacgctat ggtgtgcgcc
     2521 ggcggccgcg gcgatcgttt tgagaaactt cggttgcccc gggtcgctgt tgtcgtacag
     2581 attgacgaaa cccggtttcg ccgtggtgac gaagagacga tctcccttgg cgttgtagag
     2641 catttcgagc ggcatgccct gctgccgtgg gccgaagtca tcgacctgat ggaacgaaaa
     2701 cgtcttgctg gccggatccc agaggccggc ccacaatgtc ccttcgagca tattggtgat
     2761 atgcacggcg ggaggattcg aattcggact gaacatgact tcgacgggag cggctttcgc
     2821 cggtgacggt ttcgctgaga ccttgtgggt cgagagcacg cggccgctac cggcctccat
     2881 cacggtgatg gagtctcccg catcggacat gtccggtttg acggtactgg tcacaaggac
     2941 gcgatcgatg ccgttatgaa tcgcaatccc gtgtggatac tgaattgtgg ccaacgccgg
     3001 ctctgctgcg ctgaccgttt tgatcggctt atcgagcaag gcatctccca taatgacatt
     3061 gttcgagccc atgcaggtga gataccaggt tcggttatcc tctgacacgg ccagatcttc
     3121 tcccatctga caatccggca cgtcgatcgc ccgcagccga taagggaatt ctgtgagatt
     3181 cacgacgtgc agaatgctct tccccaaggc cgtgacgtag gctttactcc ggtcgcgatt
     3241 gaagaagata tggtgggcga cgagatcggg aggcagcgga atctccatca ggattttccc
     3301 gaagttcccg gattcgggat cgatgtccat aatggcaatg ccttcccgtc ttaccgtttg
     3361 gtccggcttg ctctcataat tgagcaacgc cagtatttcc gctgaagcca gcgtgttgca
     3421 ggccagcaat aaccccagcg tgaggatgaa tgatcgatag agtttcatga tgagctcctt
     3481 ttcaattgcg gcagcgaact cacggtgcct tcttccagtg gtggcggctc cgacatcgtt
     3541 gtccgcgcga taggcctcta ttgcagggcg ggccgacagt cccggcccgc ctcaccggtt
     3601 agatcccctt cagcgcatgg gtgccgtgcg tgacggcacc gccggcgcgc aacgcggctg
     3661 tcacgagaac cgattcagca atctcggcgg ccgaggcgcc atattctttc gccttgttcg
     3721 cgtgcagttc gatgcagtag ggacattgcg tggtgaacgc cacggccagg gccatcaact
     3781 ccttgtactt ccctgggatc gcgccgggcg ccagtgcagc cttatcgaag gccacgaacg
     3841 ccttcatcgc ctcgggtgca tgcgccccca gatctttcac cctccccaaa ttcgtcatgt
     3901 catacagacc cgtcgtctga tcgatctcgg tggtggtcgc gttcgtcatg atgcatctcc
     3961 tttgtatggg caatcggggt ttacgaccga atgccgatga cttccaaata ttcgccatac
     4021 accgtcgtgc agtccggtcc cgcttgattg tgcgcggccc acagcgtttc cagatcccgg
     4081 cgcaaatgcg cctgcccttc ggcctcaagg gaggcaaagg cccggttggt cggtccatag
     4141 tagagacgga aaaattccac gacctccgag ggaggaaacg gatagctgaa gagatactgc
     4201 cgtctggtca ggctgattgt ggacaatccg tgaccgaggc gttcctgcac cgtggcttcg
     4261 ttaccccaca gcaccggcga cggcattccc gacgggccga tgaatttcgc gacgttctta
     4321 aacatctggc ccacaaaccc ctccgccgtc caattggcca tggcaagggt cccaccgggc
     4381 gtgcacaccc gcaataactc ctgcgccacc aggtcaggcc gcggggcgaa catcgcaccg
     4441 atcaggctcg tcacggcatc aaaactggct gtttcatacg gaagcgcttc ggcatcggcg
     4501 acttcgaatc gtgctgacac cccttccgcg cgcgccctgg cttgcgcgcg ctcgacccaa
     4561 ttggccgcaa tgtccactcc cgtcacctcc aggccatcct tcgcggcgat caacgccagt
     4621 tggcccgacc cgcacccgac atccagcaac cggcaccccg gcgcaatctg caggcgttcg
     4681 taaaactccc tggcgccgcc ctccatgtat cgtgaaaatc gatcgtagtc tcctgccgtc
     4741 caaatagtat tgagacgggt cttcagacta tcgagctccg gcattatggt attcgtcgtc
     4801 atggcctcct ccttgttgaa tgtgctctgt cctcgtgcct cgtaccgcgc gctgtctggt
     4861 tgcactatag gacaggggcc gttccgcgtc ttatccgcac gtctggctct cttatcggga
     4921 gcatgctaag ctgcagatcg tttatccggg cgtccggatg actttgctca gactccggcc
     4981 tcgccgagga actgttcatg gacgtgctgt ccgaagtgct gaaggccgtg aaactcgacg
     5041 gtgccgtgtt ctttcacggt gaattcgccg cgccctggtg cgcacaagaa cctgacgccg
     5101 agaccatggc gtcgtatctc cccacccaag ccggccacat catcattttt catctgctgc
     5161 tggaaggacg gggctatatt cgcctcgggg acgaggaccg cgcggtgccg ctgacggcgg
     5221 gagacatcat cattctcacg cagggggatg cgcactatat gggcaacggg ccgcccgtca
     5281 cgccggtgga cacctctctg caaatccgtc agatcctcgc ggagggacgt atgatgtcgc
     5341 aactcgccgg cggcggggaa attacgaagc tgatctgcgg gtacctgacg tgcgacgcgc
     5401 agctatgccg ggttgtcctg gccgggctgc ccgccatgct gaaggtgaat atccgggata
     5461 cgccgtcggg acactggctg gagaatacgc tccgctactc cgtcgatcac gcagaggcat
     5521 ccggtcccgg aggcgcagcg gtgatcgcga agttatccga agccttgttc gtggagacgt
     5581 tgcgacgcta catcgcacaa ttgccgcaca ccgagaccgg ctggcttgcc ggggtgcgtg
     5641 atccggacgt gggcaaagct ttggcgctcc tgcatcaacg ccccgcccaa ccatggacca
     5701 tcgccacgct cgccgccgag gtcggtgtgt cccggtccgt gctggctgaa cggtttcagc
     5761 atttcctcgc cgacacaccg atcggctatc tcacccgctg gcggctgcac ctcgccgcgc
     5821 aactgctcac gtccacgtcc aagagcgtgg ccgaagtcgc cggcgacatc ggctatgagt
     5881 cagaaccctc tttcaaccgc gccttcaaac gggagttcaa tctgcctccg gcaagatttc
     5941 gtgcccaatc gaaggcgtcg ttggctggaa acggtgccgt ctcccggcaa gatcaccgat
     6001 cagcgcctgc caggtgaacc tccggccctt cgaatcggcc ttccatcgcc ttgcacttgg
     6061 tccacgggtg tgctgccgcg ccggcgaccg ctcccaccat ggcaaacggc atcgcgacaa
     6121 caaagagcgg cacaccgata ccatacccga gccattgcat cgcgatgctc ttgcgcttct
     6181 gtcccgcctc gacgacctgt tcggctggat acaacggaaa ggtgccgtat tcgctgccca
     6241 ggctcgcact ttcgccgatc gtccggcagc gcgcctcact catcaccggc agcaagagac
     6301 tcatgcagac acagagcact atcgttttca tcaccactcc ctccagtaaa caccaccgca
     6361 attggctgct cgccctggtg cgaaaagata ccagcacatc ccatgcctgt cgttgatacc
     6421 aacgaaacgc tgggttctat ttgagcggtt acatctcccc gcgaaaccga gcgctttcgc
     6481 gcattcggca gcgtgcgaaa acacaggctg caataaatac gcaatccgtc ctcataccac
     6541 tgtctcaaca tgaggcaaaa aaaatccttg cttctgtagg acaaccggcg tattagtcct
     6601 agtacagttg atcctgacta gaacgattca ctgcggctcc atcgcaccat gaggattcga
     6661 tcctcttggt gaatcaatac ggtttcccgg cccattgaat gagtgctcaa cgcacctgcc
     6721 atgatgcgaa tcatggatcc ctgggctctg catttctctc atacgttccg gacacgatgt
     6781 tccccggcgc tgcactttcg gcgcaggaag gaggagccat tgctattcca tacatcggcc
     6841 tctggtatcg aacagccatc cgcgcttcaa gctgataccg ccctctctgc tcgcctgctt
     6901 gtctcgtggc tggtgttctg gattctcgcc ctcccgctca tgcacgtgca cattcaagct
     6961 gagcggcatc aggggcttcc gcacaatgtg ctggccggcg atctgccggg tgagtttgtc
     7021 tcgtccgcag atagcgccat agaagcggcg caacccgctg gcgttccgcc tcctccacac
     7081 ctcgcctccg gtatttccta tccggaattg gcgttcactg cgccggttcc ttccaaacat
     7141 cattggttgt tttctctgac cgccattttc acacgtgaca ttccagcaga gatgtttccc
     7201 accggtagaa cggttactcc cgaccaacgt tcattcgatc accccactcc actccctcta
     7261 tcctctccac gcgcgccgcc gccagtcgac cggctgacct aagatcattc gcgctgaaga
     7321 gagtgattct ggagaacatg ctccaaaggg atccgtctgt tcaaaagatg gatgggagac
     7381 gactggcatt cgcacgacca ataggagatt cagcatgaaa ttatttgctt tggcagtggt
     7441 gagctcgacg acattgctct tgggctccac gggcaccgcc ttcagtgccg gtgcatcttc
     7501 agatagcaac agcgtgaagg aggcatctcc ttccgcgcaa ctgattctcg ccaagcaggg
     7561 ccgcggcgga catgatgatc ctcccgggga tgatcacgat ggcgtcgtgt tggccaagca
     7621 gggccggggc ggacatgacg atcctcccgg cgatgatcat ggagaccatg tgatcatcgg
     7681 ctagtccttg tgcagacagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 cccggcgatg atcatggaga ccatgtgatc atcggctagt ccttgtgcag acagggatag
     7861 cccatctagc gaaaacggac atctgctacc gagcatgcgt gcttcgatga aagagcgcat
     7921 cgttcgtgtg cgaaggtgtc ctccattctg atatcaaccg atgaatgcct gttgtctccc
     7981 ggggtgatgg tactgccatc accccccctt ctccccctca agttccttcc aacacaccgg
     8041 gctcctcgat gcacagaatt tgaggctttc cctcgagagg cgtagagtag cgtctcgtgc
     8101 cagagttcca aggaaagtcc gctccccgct agttcacgat aaaaaggagc atgtcatgca
     8161 cccactgatc gttctggcat tcatcgctct cgccggtggc ctgttgccta ttccagctgc
     8221 gacttttgcg caagcccttc cccccgccct catgcagaag attcaggccg cgcagcagca
     8281 cgtcaacacg attggaatgg aagcttatcg gaaagtgact gataatcctg gcgacgctct
     8341 catcattgat gtacgcgaac cacaggaata tgccgcgggt catgtgcctg gtgccgttaa
     8401 tattccccgt ggcgttcttg aatttcagat ttggaaacat gtcggatccc ctggcgcgcc
     8461 ccaaatggca aagcccctgt accttcagtg tcagagtgga aatcgtgcgt ctctcgcggc
     8521 gcaatccctc gcggaattgg gattcagcca gacaacggct gtcgtcatga gccttgagga
     8581 gtggcaaaaa gccgggcatc cattcgtgaa gtaatccata ccctgtcgag aagacacgga
     8641 ccgcatctgc cgcgttgagc aacctccgga atcgcgcgtt ttcgctcact ccgctctgtg
     8701 cggaatcgat agtctgccga ctttccctca aaaatttgcc gcgcacctat caggttgaac
     8761 attccagctt caggacctaa gggtccagcc ctgcgtctac atcttcttcc cgcccatcgg
     8821 gtgaatcacc gtgatagcca acccttcaat aaggcaccgg gcttgcttat caattatgag
     8881 caaacacccg ggctgttttc atgctgagcc gatccaagcc gggtaggcag caggccatag
     8941 ccgtttcttc tcattccgct gtagtgattg tttgacaaaa ttcatgccga tacctcagcc
     9001 ccccacagcg tctcgactgg cggccacgcg atcatccgga cgcccgctcg tgagtgtcat
     9061 cattccaacg tacaatcggg caccgctact caagacagcg atcacctccg cattacacca
     9121 ggagcgagcc ggcgatctgt ttgatgtgga agtcatcgtg gtcgatgact gctcaccgga
     9181 agacatgagc ccgatcgtga gcgagtttcc aggcgtgcgg tatatccgct tgcctgaaaa
     9241 tcgcggggca tcgggtgcca gaaatgccgg catcaaagag gccagtggca agtatgtcgc
     9301 cctcttggat gatgacgacg aattcctcac ccataaactc atgatccagg tgccggtcct
     9361 cgaggccgat ccggacgtgg gcgtcgtcta tgggcaaagt gtcgtcacgg gcagcgatac
     9421 gcccctgctc ctgtggccgg aatggggacc gtcaggacgg gtattcgaag agtttctgac
     9481 gcggacggaa gactttcttc accctcccac gtggctcgta cggcgggagt tgttcgacgc
     9541 ggcgggatgg ttcaatgaac aacatcgaac catggaacat tacgacatgg cgctccgcct
     9601 gtcgatgctg acctcctgga catttctcag cggcgggccg gtcgcgcgtg gtcgcttttc
     9661 caagaaggga aagtggtaca gcaatatcgt caacggcacc aacgaggaac ggctcccgcg
     9721 aattgtggag agcgccctgg ctcgattacc cgcgactcct gaagccgaac gcactcgccg
     9781 caaagctcgc gccgcggtct gtgccacgat tgccggccaa cgatggtgga cgggcggcgg
     9841 gcttgaaccg accaagcagc atctgctggc gtcgctccgc acctcagcgt ggctgctgca
     9901 tgaagcaccg gtgatggagt ggattcaacg gatcgcgggc acagtggccg gagggtcgca
     9961 tcagccggtg caggcggtgc gagccttctg ggaagaaatc aaacaagcca cctccgccgc
    10021 cccggacgct cacaccattc ccatgcgggg agtgttggga gacatgctct cgagcgcggc
    10081 catcaccatg aaacaagggt caccacggcg agcatggatg gtgggactcg gctccatcgt
    10141 ccataatccc aagtcgctga aacaaccggc acggctgctc cacttgtatc gcgcactggc
    10201 cacgactccc gcgacatcgg ggactcgact tccgatttct ccctcgtaag gtgagaggtc
    10261 gttgtttcgc cggcctccgt cgggagagcc gactgcagac cctaccaaaa cccgcctcac
    10321 ctcatctccc ttgccaggat gactggccaa atgctcgcac cgcaagatac gccacattac
    10381 gtttccaata ggcgaccttg gatctggtca tcaattccag gaacacgtca tcggcatctt
    10441 tccgactgaa cagcttgccg gcagggatca cctcgccgtc cggaagaatc acctgtcctc
    10501 cgcagcgata gatcaaatca tgcatgatcg gagcgaccag cgagagctca aatgacgcaa
    10561 tgaccgccca aaaaagacgc gggatactgg ctaagtcgct cttaaagccg cgcttggccg
    10621 taatgataca cccatcgagc gcaacatacc ggcaatcttc cgccacgacc cacaggtgcc
    10681 catcatgttt cacaaccgga tcaggaacgt tgggtatcag atcagcttca tccctcctgg
    10741 tttgtcgcgt ggcatgtgta aagtgacgct gcacatcggc agcagaaata ttcctattgg
    10801 cctgcccgag atcaaagaca aacgttttgg tcagcgcggc aacaaggcta tggttgtcgg
    10861 caagcatgga ggagacacct cccgttggag acttggctgg cggggcgctc tcggtgacat
    10921 gctcacggct gactacaagc gacagggtgc gtccctggtc actcaagttc aaggtcacgg
    10981 tctcgcctcc ttcgattcgc ttctcgatac ccgcaaactg acttataagc tcagtgagat
    11041 aggcattcgc ttgcgagaac tgcatctgta ccggatcggg catacctgtc tcctcggttt
    11101 ggtgcatccg tcacatcgac tgcggcatca tgcctaaacc gatagacctt cctcaagcct
    11161 tcccctcaca cgaagtggcc aatttctgtg aggaaagggt atccagctga cacatcgaaa
    11221 ggcatctgca agcgaacaga tatcgcgcgc cacgaaacac gcgaaaggcg tgaaatgaga
    11281 tgtcaggaaa tgaatcctag aggagcgtca ctgcggcggt aggtggagaa accgccgccc
    11341 ccgcatcaca ggcgaaggcg gctgaaagct ctctggggcc acagttcgcg agggccacca
    11401 gaacaggtca ctccgacagg ggactaggga gtggaaataa tcggaatttc tcgcgcggat
    11461 tcttccttaa actctgccat cttcttgtga acggcctcaa ggatttcagg attgctgcgg
    11521 caggcttcac tgcacttttc gcccatctgc agaatcgaac aatgctcccg gaccgttaac
    11581 ttcccctgca cgccattcat ctcgaagccg accttcaccg gctcctgatg cagcggacag
    11641 agggtctcgg ccacaatcat gtccttgact gtatgtggat gccttgcaaa taggttcgcc
    11701 atatggactc cttactgtgt gacgggtgaa tagtggtaca gtggcctccc acatcactgt
    11761 atcaagagac ccctccggca caaacttgta tcgcatttgc agcgtaccca cgccacggct
    11821 gcatccccga cccccactgt attagactgg acgcaatcac actttccttc agtggctgtc
    11881 acaaccgagg agactcatgc cttacgtgtt gatcattcat gaagtggacg cctatccggc
    11941 gtggaagtcg attttcgatc aggcggcaca gatcagaaaa caagccggtg agatcagata
    12001 ccagctcctg caacatgaca ccaatcccaa ccggctcgtg catttctcag aatggacgtc
    12061 gctcgacaag gcccgcagct tttttgagtc tccggagctg gtcgagattc ggcgcaaagc
    12121 gggagtcaag gcgccggcat tccactattt gaatgaggtc gagcagggcc ggttgtgaat
    12181 gccgcgacct gagtcgcctc gacggcctgg tattcacaca catttccgac ctttgctcac
    12241 gcttttcatc agtctgggat atgctgaccc ttcacacgct gtgcgaatac cgatagcgcc
    12301 atcaaccaac aacgaggtca tcatgcgatt cgtaaaagta aaagacgagg aacggcccgg
    12361 tgaagttgcc atcaatcttg acctggtgcg agaagcgcat ttcggaggcg gcttgctgca
    12421 cctgtacttc gagcgcagtt cctcggcgca ggatgacatg acctttaccg gcgagaacgc
    12481 ccaaaaaatc tgggccgcga tggggtgacg agacggccct attcctcgac ctctccagca
    12541 ttcccttttc cttgcatcct ccctctgtcg caggctgtga ctcacgccca cgctctgctc
    12601 gattcgtgtt atagctagtc atacggaggc cattcgcctc gacggcttgc tgttggcaca
    12661 tcaactcgag gacctcatgc cgaatcatcg tatcgacctc aaacgtcttg cccgacacgc
    12721 gatgattgat cgcggcttgt ggccagactt ctccgccaaa gccatggctg agctggccca
    12781 gatccgcgcg gcagcggtcg accatggagc ggacgcgcaa gacctccggg acctgctgtg
    12841 ggtctccatc gacaacgatg actcgctcga cctggatcaa attacggtgg ccaccgaaca
    12901 tccagatcac tcagtcaccc tgctggtggc gattgccgac gtcgatgcgc tcgtacccaa
    12961 agattccgct atcgataccc acgcgcgcca caacaccacc tcggtctaca cgtcgggcga
    13021 tattttcccc atgctgcctg agccgctttc tcacgatctg acctcgctcc gcgaacatca
    13081 ggaccggctg gctgttgtgg tagacctcgc catcgccgcg aatggaaaag tgagcggggc
    13141 gtctcattat cgggcgttgg ttcgcaatca cgccaaactt gcctacaaca gtgtcgccgc
    13201 ctggctgacg ggaacaggac cggcacccga tagcatcagc gacgtgccgg gcctcgacgc
    13261 gcaactgcgc cttcaggatc gcgtggcgca acagctcaaa gcacgacgac accagcaggg
    13321 tgcgctgagt ctcgaaacca tcgagccacg cgccgtcttt gacggcgata ctctgaccgg
    13381 catcacggtc gaacagaaaa atcgtgcgaa ggagctgatc gaagattgta tgatcgccgc
    13441 caaccaggcg accgcttcct atctcaaacg caaaggcatc ccctccttcc ggcgcatcct
    13501 tcgctccccg gaacgctggc agcggatcgt cgacgtggcc gcaagttgtg gcgagtcctt
    13561 gcctccgcag cctgatgcgc aggcattgga tgccttcctc gtcaaacggc gagcggccga
    13621 cccgatccgg tttcccgacc tctcactggc catcgtgaaa ctgatcggtc gaggcgaata
    13681 tgtgctcgat gagggaacgg gcggcgcgcc ggaacatttc ggactagccg tgaaagggta
    13741 tacgcattcc acggcgccga accggcggta tcccgatctc gtgacccaac gattgctcaa
    13801 agcagccctg gcgggcgcgc ccctgcccta ccggacggat gaactgaaat atttggccga
    13861 ctattgcacc gagaaggaag atgatgcgga acgggtggaa cggcaaatga gaaaatccgc
    13921 ggccgccctg ctcttgcagc ctcgtatcgg cgaacgattc gaggccattg tcaccggcct
    13981 gtccgagaag ggaacatggg tgcggctgct tgagcccccg gtcgaaggaa agctgtccgg
    14041 aggcgtgatg gggttggatg tcggcgatcg tctgaccgtc gaactggtga gcacgaatgt
    14101 cgaacggggt ttcatcgact tctcgcga
//
LOCUS       DAOQEW010000094        14069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1224_length_14069_cov_9.145783, whole genome shotgun sequence.
ACCESSION   DAOQEW010000094 DAOQEW010000000
VERSION     DAOQEW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14069
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_14069_cov_9.145783"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLS35_11135"
     CDS             <1..262
                     /locus_tag="PLS35_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19265.1"
                     /translation="TNGVTSVRVLCAWCVRDGKPAFLREKLPLDDLSETHGLCEDHFT
                     SLSRSVKQVVTPQVWLLSRLNDLSWGLTRWIQRQMGRLLFLS"
     gene            complement(305..715)
                     /locus_tag="PLS35_11140"
     CDS             complement(305..715)
                     /locus_tag="PLS35_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNI19266.1"
                     /translation="MGLFDQLGQAATGMLGQTGNNNPLLQAVVGLLAQNSHVGGLGGL
                     IQAFQKNGLGDIVNSWVGTGKNLPISPEQIHQGLGGDLLKQLASQAGLSTEAAGGQLA
                     NLLPGLIDKLTPGGKIPDSTLIEQGLTLLRGKLG"
     gene            complement(749..1285)
                     /locus_tag="PLS35_11145"
     CDS             complement(749..1285)
                     /locus_tag="PLS35_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HNI19267.1"
                     /translation="MSITALQSIKKPWAHLLVHAEGEALDKLLSVPAQFVTKTVSGKK
                     CKTKAGLLDEFARVFSFPDYFGHNWDALEECLADLDWLPGKGYLVVVTDADQVLTKPD
                     EEDDFETFVEILSEAGEAWSLKESDDATGNGLPFHAVLAVQERHKRTRHNWFAPPLAM
                     ERKPTKSGGSKGSKTSGR"
     gene            complement(1292..1753)
                     /locus_tag="PLS35_11150"
     CDS             complement(1292..1753)
                     /locus_tag="PLS35_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HNI19268.1"
                     /translation="MSVSRVTRLVLWVCATLLLHLGVAFTADPGPALLHTPVPHLTPA
                     PPQTPPADDGAVRAGPGSKHNEVRDTIPSAARDTLKAIEARHGEPLPGYVGGRNFLNR
                     ERVLPRGRYREYDVHPKVPGKNRGAERIVINQSSGKAYYTADHYRSFVPMN"
     gene            complement(1847..4186)
                     /locus_tag="PLS35_11155"
     CDS             complement(1847..4186)
                     /locus_tag="PLS35_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNI19269.1"
                     /translation="MSASDKQATEQLDFVTLEGLLTYGTALAQRSSSGRYALPPPPPP
                     SADRLQPAQAAVEGMRAFIQHVQKGLPSAEAYRAARQTLLAKTCGNDPVVFFAAWNLL
                     LARGELAPLYRAPIGATQKPAHRRPVAIVPRTQLTPQLAEGRIVLDLGDDRFWLLPRD
                     LGDRTLFLTMRHGVSEVESKTHRVGRRLANVLDTERGVPRADAVGAALARMVGVVGQQ
                     LGYLHLRNYLDPRTFLHLISHSPNTEQLCRRITKALLPDGADAVHPHIEPTLESQDFG
                     WVTGMEKQAELDAAAQAFGVDAKTAKRLIKHPFYSYPGGHSFFDLYVDVIDGLHRLGH
                     AHPGEVLCLYTHSSTLRALRIYLDPRPFREAFSEFGEYKEGQDNVVLLTLENGKLSGY
                     STAVGLIESDRVAREAWVTVERERTQRVTLKPGRIKRLIALVSGGDFGGGGAALKELR
                     VTGNRFGLDVFFVRHGFLGLANNWIDAVTEEDTRGMSSHASSPIGSSRFEDFKDEQVQ
                     RAAMRSLAPYLEDSALVVLGGDGSLRGARAIHETYGVQVVGIPGTIDNNIAGTTSLGF
                     HSAIALANQSIESLKATSAAMGSIFFVEVMGAGSGHLALACAYQARAEGILVNEHPDP
                     DTYIEDIVLGTLQRTLGVPNKSHIFVVAERTPHRHHREGGVHGLVDYVAQTIATWPQR
                     RDATGPYSLASATKATILGHTLRGAPPTPEDKMIAQHLAYETVRRLVEQPDTIVGCMV
                     AYHDSNRIETIPLHAVSPKPFDWELFTRMHVTELQHDRV"
     gene            4394..5845
                     /gene="pyk"
                     /locus_tag="PLS35_11160"
     CDS             4394..5845
                     /gene="pyk"
                     /locus_tag="PLS35_11160"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNI19270.1"
                     /translation="MRKAKIVCTIGPASDGVGVLEQLIQSGMNAARLNFSHGTHASHG
                     QAIKVIREAADRQGVAVAIIQDLQGPRIRVGEIDGTLELRAGQRVRLRTMTLRSGGQI
                     GARNVLPADSAQDIPVTYQALTRDIRPGAKILIDDGLVELTADRIIDGAVECTVVAGG
                     RVTSHKGMNLPGTIVSAPTLTDKDREDLRFGVAQGVDYIALSFVRGADDIRAAKQLIA
                     ECGGDVPVIAKIERHEAVTDLDAILQQADGVMVARGDLGVELGPEAVPVLQKRIIATA
                     NRHRRLVITATQMLESMTQHTRPTRAEASDVANAVFDGTDAVMLSAETAVGHYPVEVV
                     QVMDRIVRAAEVETGPAFVRRASPDNRHLTFEEAICLSASSAAAATGATAIVAFSERG
                     TTARLVSKQRPAAPIIGFTPFEPVRRQMALYWGVMPHTMPQIPTTDERVNEAERRLKA
                     DGLATAGQRIVILSGTRIGQPGGTNLMKLHEVG"
     gene            5850..6410
                     /locus_tag="PLS35_11165"
     CDS             5850..6410
                     /locus_tag="PLS35_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19271.1"
                     /translation="MISLRGAIIGIMAGIVGLTAWASPGHAQSNAVSHSPAKVVEKYF
                     ALDNKGVRLDASSFESVAGYVDWKEEPAWGKVVVINGFTVPDDFRQWEIVNRLEVVVP
                     VEFRVLGIMYLDTAGFVPEPGTEQARVRLKVMNGRWKIMEPILPPHVGQKRMLNVVRQ
                     AMLEEKDGTRQASLAALQAELRKAKE"
     gene            6407..7057
                     /locus_tag="PLS35_11170"
     CDS             6407..7057
                     /locus_tag="PLS35_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNI19272.1"
                     /translation="MTKKSEIDLLIFDLDGTLIESKWDIADSVNLTLRDLDLPERPEE
                     EIFGFVGDGVKKLLRLAVGEGNQDRFDQALRVFRGHYLEHCLDRTVFYPGIDRVLAHY
                     AAKSKAVATNKAIEYTNVILKGLGHHHFAYVVGGDNGFGLKPEPGMLVHVMEQLGVKK
                     DRTVLIGDSTNDINGGHNAGIRVCAVGYGMGNRQKMAACQPDWFIERPEELMELFI"
     gene            7054..8625
                     /locus_tag="PLS35_11175"
     CDS             7054..8625
                     /locus_tag="PLS35_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNI19273.1"
                     /translation="MISITRARTIGVVILSGILAAAAPGYAESPSLADRVIEHKLANG
                     MTVLMVERHQAPIVSINMTFGVGGVNEQVGLTGLAHLYEHMAFKGTRTVGTRDYEREQ
                     AVLDELTVVGNELDHRERDEAARAQMEGKPHVASPEVQQLQRRFKELQEKAGEFVVGN
                     EMALLYQRHGGVGLNASTGKDITRYVISLPANRLPLWASLESDRMAHPVLREFYKERG
                     VVMEERRLRTDDSPNGLLYETFTSTAFQAHQYGVPTIGWGSDIMALTPAATEAFFKTY
                     YGPNNATVAIVGDIQPKEVIALIEQTFGKIPAAPPIPALVTEEPPQRGERRVEIEFDA
                     EPALAIGYHKPTIGHPDDFVFDVIDEVLTEGVTSRLYSTLVRDKRLAASVLSDTNYPG
                     VRAPNLFVIAATPLAPHTTAEVESAIYEELERLKTEPISAKEFERVLNGLDADLVRSL
                     RSNSGLASQLAFYQTVAGSWRYVLNARARIAAVTPADVQRVAAQYLTKPNRTVGVLVK
                     KAPDKKMAAVGEVAR"
     gene            8682..10109
                     /locus_tag="PLS35_11180"
     CDS             8682..10109
                     /locus_tag="PLS35_11180"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNI19274.1"
                     /translation="MSCPVFVLLFLVQASLVMAGDLVLDNPRSMRFPTVEFNPPDAER
                     LVLDNGMVVYLLEDHELPLVTINATLKTGSWLDPADKVGLAGMTGAAMRTGGSASMSP
                     DEVDEELEQLAATISIGFSKESGSASLDVLKKDLKRGVQIFSDLLRRPAFEQSRVELA
                     KLQALEGIRRRQDSPGSIVGREFAKLLYGPTHPSARETSVRSIESITREDLVAFHQQT
                     VHPNGIILGVTGDFDKAGMLALLREAFGDWAKGTVPDVTIPAVAESEFKTGVVRFVNK
                     DTSQTHLRVGHLTVKETHPDYVAVAIANDILGGSSFRSRLFNDVRTKRGLAYSVGSGL
                     RASVYDEGVWLMRAETKLSSTQEVVNRFVANMERMRNEPVTDAELEEAKEAYVNSFVF
                     SFTSASSIVARLMDLEYDGLPKDWLQQIRDKVVKLTKEDIQRAAKMHFNPDRLRILAV
                     GSGDALSKVLASFGEVKEITLAPES"
     gene            10315..11151
                     /locus_tag="PLS35_11185"
     CDS             10315..11151
                     /locus_tag="PLS35_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI19275.1"
                     /translation="MPLEDELSDIIKKARTGQQRSVADVARAAGFPEADLAGLERGQP
                     ARNREQVQAVGRALGLRAEQLAQVVEGWTPEALPASTPHVDTILGSIGGYEVKGYIVH
                     DRGEAVIVDTGYNADAMLEMLSKRALRLRAICLTHGHSDHADGIDAILKAHPVPVYLG
                     PDDLDLLSWRPSQDVLHVPKEDGTLQVGGLRLRFMPTPGHTPGGVCYRLEQAGEPMCF
                     VGDTLFAGSIGRSNPAGLYQTHLASVRQRVLALPPDTRLFPGHGPATTVHEERLHNPF
                     AT"
     gene            11167..12126
                     /locus_tag="PLS35_11190"
     CDS             11167..12126
                     /locus_tag="PLS35_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNI19276.1"
                     /translation="MNRDESDQNRSSGELISPSFENAEEDEWVEEERPAFSAFVLPIM
                     LFALTLFTVLWAGAYQTNTNPLVGPWDFLVDDPGSLWRGIPFAATLLGILVTHELGHY
                     VLSRLHGVPTSLPLFVPGLPHFVGTFGAIIRMRAPLTDRRALFDIGVAGPIAGFVVAV
                     IALVIGLRLSTVVPIQGNYGMHLGEPLLLQFASWVVIGPLAPTADVILHPVGFAAWFG
                     LFITSLNLLPIGQLDGGHVAYALLGERQRSVAVALIPILMVFGWLGWKGWFLWVGLAG
                     MMGLAHPPVRNPGRDLGTARVLIGWLALFIFAVTFSWEPFILR"
     gene            12130..12852
                     /locus_tag="PLS35_11195"
     CDS             12130..12852
                     /locus_tag="PLS35_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19277.1"
                     /translation="MHCPKCGWDQDEGTADCARCGIIFAKMHRSPAPPVMPRSPGQDS
                     TRPRVVEEWLLITEDSVNPFYFAGRVLVFLLLVSWGWRFITTPLETNYTGESFLHLIN
                     LPFHEAGHLIFMPFGRFMMILGGSLGQILMPLICLGTFLLKTRDPFGGSVALWWTAQN
                     FMDVAPYINDARAMDLILLGGFTGKEVDAHDWNNLLTMLGWLQYDHGLAKLAYGVGTV
                     LMLVSLLWGASLLRRQYRRLDW"
     gene            12943..13398
                     /locus_tag="PLS35_11200"
     CDS             12943..13398
                     /locus_tag="PLS35_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNI19278.1"
                     /translation="MFASLIFLWAGVSLSAVHAEEAASRFTLVLNGEAVKDSASGLVW
                     EREPDYIFDVWDRSVARCATKTVGGTQGWRAPSIDEIKTLVDLDQQDPSLPAGHPFRN
                     IKSGIYWTATPHPKDEIVAWQQSFFSGQSVTDQKSGTRRLWCVLGDAGK"
     gene            13650..>14069
                     /locus_tag="PLS35_11205"
     CDS             13650..>14069
                     /locus_tag="PLS35_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19279.1"
                     /translation="MTTLLTVLLGCYLLGGLLPLCTSRPHLQNILAHSAAALAGGAGI
                     ALGLLGLVASQALTISVPSNIPYLTFALRLDPLAAFFVLTISLIGMAASIYAYGYVRE
                     FTGRVSIGLLGSLLNGFLLSMTLVVLADNGFFFLIVWE"
BASE COUNT         2707 a         3943 c         4440 g         2979 t
ORIGIN      
        1 aacgaatgga gtgacgtcag tgagagtgct ctgcgcctgg tgtgtgcgag atggaaagcc
       61 ggcctttctc cgcgaaaagt tgccgctcga cgatctcagt gagacgcatg ggctgtgcga
      121 ggatcatttc acctcgctga gccggagcgt gaagcaagtc gtcacgccgc aagtctggct
      181 gctctcgcgc ctgaacgatc tgtcctgggg actgacacgg tggatccagc gacagatggg
      241 acggttgctc tttctctcct aggcatctgc acgccggtta gccagaatca gccggccatc
      301 gaatctaccc gagttttccc cgcaacaacg ttaacccctg ttcaatcaac gtcgagtccg
      361 gaatctttcc tccaggtgtc agcttatcga tcaaaccggg tagcagattt gccaattgcc
      421 cgccggcggc ctctgtactg agacctgctt gggacgccaa ttgtttgagg aggtcgccac
      481 ccaatccctg gtggatctgt tccggtgaga tcggcagatt cttgcccgtg ccgacccagg
      541 aattcacgat gtctcccagt ccgtttttct gaaaggcttg aataagtccg ccaagccccc
      601 ctacatggct gttctgcgcg agcaggccga ccacggcctg gagcaatgga ttgttattgc
      661 cggtttgtcc cagcatcccc gtcgccgctt gaccaagttg atcgaagagt cccatcagtg
      721 cctccttaca tcacgggaat ggtacggtct aacgaccgct ggtttttgat cccttggatc
      781 cgccggattt cgtcggtttt cgctccatgg ctaggggcgg tgcgaaccaa ttatggcggg
      841 tccgtttgtg ccgctcctgg actgccagca ccgcatggaa cggtaggccg ttgccagtcg
      901 cgtcgtcgga ttctttgagg ctccaggcct caccggcttc gctcagaatc tcgacgaagg
      961 tttcaaagtc gtcttcctcg tcgggcttcg tcagcacctg gtctgcatcc gtgaccacga
     1021 cgaggtagcc tttgccaggc agccagtcca ggtctgccaa acactcttcc aaggcatccc
     1081 agttgtggcc gaagtagtcg ggaaacgaga agacccgcgc gaattcgtcc aagagcccgg
     1141 ccttggtttt acattttttc ccggagacgg ttttggtgac aaactgggcg gggaccgaca
     1201 gcaatttatc cagcgcctct ccctcggcat gcaccaggag atgggcccaa ggtttcttga
     1261 tcgattgaag cgcagtaatc gacatgtgtc ctcaattcat cggcacaaat gaacgatagt
     1321 gatcggcagt gtaataggct ttcccgctag attgattaat gacgatccgt tcggctccgc
     1381 ggtttttccc cggtaccttc gggtgcacat catattcgcg ataacggcct cgtggaagca
     1441 cgcgctctcg attcaggaag ttgcgccctc cgacgtagcc cggcaagggc tccccgtgtc
     1501 gcgcttcaat cgctttgagg gtgtcgcgtg cagccgaagg aatggtatcg cgtacctcat
     1561 tatgcttgct gccggggcca gcgcgcacgg cgccgtcgtc cgcaggtggc gtctgagggg
     1621 gtgccggagt gagatgcgga accggagtgt ggagaagtgc agggccaggg tcagccgtga
     1681 aggcgacgcc caggtgcaag agcaacgtcg cgcaaaccca gaggacaagt cgtgtgacac
     1741 gcgagacaga catggtgatt cacaactcgt gacagcaact ggaaagaccc taacacgtca
     1801 cataggagcg gtcaacgtgg ccaccgcacg acggaagagc aacccatcac acacggtcgt
     1861 gctgcagctc cgtgacgtgc atccgggtaa acaattccca gtcgaacggc ttgggcgaca
     1921 cggcatgcag cggaatcgtt tcgatccggt ttgaatcgtg ataggccacc atacagccga
     1981 cgatggtgtc cggctgttca acgagccggc gaaccgtttc gtaggcgaga tgttgcgcaa
     2041 tcattttatc ttccggagtg ggtggtgcgc ctctcagggt atggccgagt attgtggcct
     2101 tcgtagccga ggccagcgag tatgggcccg ttgcgtcacg acgctgaggc catgtggcaa
     2161 tagtctgtgc gacataatcg accagcccgt ggacaccccc ttcccggtga tgccggtgag
     2221 gggtccgttc cgccacgaca aagatgtggc ttttgttcgg cacccccaag gtgcgttgta
     2281 atgtgccaag gacaatgtct tcgatatagg tgtcgggatc gggatgctca ttgaccagga
     2341 ttccttccgc cctggcttgg taggcgcaag ccaaggccag atgaccggac ccggcaccca
     2401 tgacctcgac gaagaagata ctgcccatgg cggcgctggt cgctttcagc gattcaatgg
     2461 attgattcgc gagggcgatg gctgaatgga atcccagcga ggtggtgccg gcgatgttgt
     2521 tgtcgatggt gccggggatg ccgaccacct gcacgccata ggtctcatgg atggcccgcg
     2581 cgcccctcag gctgccgtcc cctcctaaga ccacgagcgc cgagtcttca aggtacggag
     2641 cgagggaccg catcgctgcg cgttgcacct gttcgtcttt gaaatcctcg aaccggctgc
     2701 tgccgatggg gctgctggca tggctgctca tgccgcgtgt atcttcttcg gtgacggcat
     2761 cgatccaatt gttagccagg ccgagaaatc catgccggac aaaaaagacg tccaggccga
     2821 agcggttgcc ggtgacgcgc aattccttca aggccgcccc cccgccgccg aaatccccgc
     2881 ctgacaccag ggcgatcagt cgtttgatgc gcccgggttt aagcgtcacg cgctgagtgc
     2941 gttcccgctc gaccgtcacc caggcctcac gcgcaacccg gtcgctttca atcagcccaa
     3001 cggcggtcga atagccggac agtttgccgt tttccaacgt gaggagcacc acgttgtcct
     3061 ggccttcttt gtattcgccg aattccgaaa aggcttcgcg gaagggacgc ggatcgaggt
     3121 aaatgcgcag cgcgcgcagg gtcgagctgt gtgtatagag gcagagcact tctccgggat
     3181 gggcatggcc cagccggtgc agcccatcga tcacatccac gtagagatca aaaaatgagt
     3241 gcccgccggg gtagctataa aacggatgtt tgatgagccg tttggcggtc ttcgcgtcca
     3301 ccccgaatgc ctgtgccgcc gcgtcgagct ccgcttgttt ctccatgccg gtgacccacc
     3361 cgaaatcttg cgattccagg gtcggttcaa tgtggggatg tacggcatct gccccgtcgg
     3421 ggagcaacgc cttggtgatg cggcggcaga gttgctccgt attcgggctg tgactgatga
     3481 ggtgaagaaa cgtgcgcgga tcgaggtaat tccgcaagtg cagatatcct aattgctggc
     3541 cgaccacgcc caccatgcgg gccagtgccg cgcccacggc atccgctcgg ggcacgccgc
     3601 gttcggtatc gaggacgttg gccagtcgcc ggcccacacg atgagttttg ctttcgacct
     3661 cagaaacacc gtgacgcatg gtgagaaaca atgtgcggtc gccgagatcg cgtggaagca
     3721 accagaaacg atcatcaccg agatccagca cgatacgacc ttcggccagc tgcggagtca
     3781 gttgcgtccg cggcacgatg gccaccggtc ggcggtgcgc cggtttctgg gtcgcgccga
     3841 tcggtgctcg gtacagcggc gcaagttccc cgcgtgcaag cagtagattc caggcggcga
     3901 aaaataccac ggggtcattt ccgcacgtct tggcgaggag ggtttgtcgc gcggcgcgat
     3961 aggcttcggc gctaggcaga cccttttgaa catgctggat gaacgctctc atcccctcga
     4021 ccgccgcctg cgccggctgc agtcgatcgg ctgacggcgg gggtgggggc ggtaatgcgt
     4081 accggccgct cgatgaacgt tgcgcgaggg ctgttccgta ggtcagcaac ccttccaacg
     4141 tgacaaaatc caattgctcc gttgcctgct tgtccgatgc gctcatgatc gcctcgtggt
     4201 gtgtggcttc ggaagaaata tttcatgagt ccggcctcga gagtcctccg gacgccatgc
     4261 agatgttctt ttgcggcata cggacgggtt tgttagaatg acccgttgcc gaccgaatca
     4321 ggaacgtttc gcgcctcacc gcatacgtac aagatggtgt gccgggccga tcttgtcaag
     4381 acagaagaac agcatgcgaa aagcgaaaat cgtctgcacc atcggccctg cgagcgatgg
     4441 cgtgggcgtg cttgagcaac tcatccagag cggcatgaac gcggcgcggc tgaatttttc
     4501 ccatggcacg catgcctctc acggtcaggc catcaaggtc atccgcgagg ccgctgaccg
     4561 ccagggcgta gcggtggcga tcattcagga cttgcaggga ccgcgcattc gcgtcggaga
     4621 gatcgacgga acattggaac tgcgagccgg ccaacgggtg cggttgagga cgatgacctt
     4681 gcgatccggc ggacagatcg gcgcgaggaa cgtcttgccc gccgacagcg cgcaagacat
     4741 tcccgtgacc tatcaagcct tgactaggga cattcggccg ggggccaaaa tactgataga
     4801 cgacggcctc gtagaattga cggctgatcg cattatcgac ggcgcagtgg aatgcacggt
     4861 ggtggcgggc gggcgtgtca cgtcccacaa gggcatgaac ctcccaggca cgatcgtcag
     4921 tgcgccgacg ttgacggaca aagaccgtga ggacttacga ttcggcgtgg cacagggcgt
     4981 ggattatatc gccctgtcat ttgtgcgcgg cgctgacgac atccgtgctg ccaaacagtt
     5041 gattgctgag tgcggcgggg atgtgccggt gattgcgaaa attgagcggc atgaggccgt
     5101 gacggatctg gacgctatcc tccagcaggc ggacggggtg atggtcgcgc gcggcgattt
     5161 gggtgtcgag ttggggccgg aagcggtgcc ggtgttgcag aaacgcatta ttgccaccgc
     5221 gaatcgccat cgacggctgg tgatcaccgc cacgcaaatg ttggagtcga tgacgcagca
     5281 tacgcggccc accagagcgg aagcgtcgga tgtggccaat gcggtgttcg atggcaccga
     5341 tgcggtgatg ttatctgcgg aaacagccgt cgggcactat ccagtggagg tggtgcaggt
     5401 gatggaccgg atcgtacggg cggcggaggt cgaaacgggt ccggcattcg tacggcgggc
     5461 atcgccggat aaccggcacc tgacgtttga ggaggcgatc tgtttgtccg catcgtccgc
     5521 ggccgctgcg accggtgcca cggccatcgt ggcgttcagc gagcgtggca cgacagcccg
     5581 gctggtttcg aagcaacgtc cggctgcgcc gatcatcggc ttcactccgt ttgagccggt
     5641 ccgccggcag atggctctct attggggggt catgccgcac accatgccgc agattcccac
     5701 caccgatgag cgagtcaacg aggcggagcg acgcctgaag gccgatggtc tggctacggc
     5761 agggcaacgc attgtcattc tttcaggcac cagaatcggt cagccgggcg ggacgaatct
     5821 catgaaactg catgaggtgg gttagcggta tgatatctct gcgcggggcg atcatcggaa
     5881 tcatggcggg aatcgtcggg ctgacggcct gggcctcgcc agggcacgcg caaagcaatg
     5941 cggtgtcgca ttcaccggcc aaagtggtgg agaaatattt tgccctcgac aacaaggggg
     6001 tgcgcctcga cgccagttcc ttcgaatcgg tggccggcta tgtcgactgg aaggaagagc
     6061 cggcctgggg gaaagtggtc gtcatcaatg gcttcacggt gccggatgat ttccgccaat
     6121 gggaaatcgt gaaccggctg gaagtggtgg tgccggtgga attccgcgtc ctgggtatca
     6181 tgtacctgga taccgccggg ttcgtgccgg agccgggcac ggagcaggcg cgagtgcggc
     6241 tgaaggtcat gaatgggcgg tggaaaatca tggagccgat tctcccgcct cacgtcggtc
     6301 agaaacgcat gctgaatgtc gtacgacagg cgatgctgga agaaaaagac ggaacgaggc
     6361 aggcctcgct ggcggccttg caggcagaat tgcgaaaggc aaaagaatga cgaaaaagtc
     6421 tgagatcgac ttgctcatct tcgacctgga cggcaccctc atcgaatcga aatgggacat
     6481 cgccgatagc gtcaatttga cgttgcgcga cctggatttg ccggaacggc cggaggagga
     6541 aattttcgga ttcgtcggcg acggcgtgaa gaagctgctg cggctggctg tcggggaagg
     6601 aaaccaggat cggttcgatc aagcactccg cgtcttccgc gggcactatc ttgaacattg
     6661 cctggatcgc accgtgtttt atcctggtat cgaccgggtc ctcgcgcatt acgccgccaa
     6721 gtccaaagcc gtggcgacca ataaagccat cgaatatacc aatgtcattt tgaaagggct
     6781 gggccatcat cacttcgcct acgtggtcgg cggcgacaat gggtttggtc tgaaaccgga
     6841 gccgggcatg ctggtgcacg tgatggaaca actcggcgtg aagaaggatc gcacggtgct
     6901 catcggcgac agcaccaatg acatcaatgg cgggcacaat gcgggcattc gggtctgtgc
     6961 ggtgggatat ggcatgggca atcgtcagaa gatggcggct tgccaaccgg attggtttat
     7021 tgaacgaccg gaggaactga tggagctctt catatgatca gcatcacacg agcgcgcaca
     7081 atcggtgttg tgatcctctc tggcattctt gcggcggctg cgccgggata cgcggagtcg
     7141 ccgagcctgg ccgacagggt catcgaacat aagctggcta atggcatgac cgtgctgatg
     7201 gtcgaacggc atcaggcgcc gatcgtcagc atcaacatga cgttcggggt cggcggcgtg
     7261 aatgaacagg tggggttgac cggcttggcc cacctttacg aacacatggc gtttaagggc
     7321 acacggacgg tcggcacgcg agattatgaa cgggaacagg ccgtgttgga cgaactcacc
     7381 gtggtcggga atgagcttga ccatcgcgag cgggacgagg ccgctcgcgc acagatggaa
     7441 ggcaagccgc atgtggcatc gccagaggtg caacagctgc agcgccggtt caaggagttg
     7501 caggagaagg cgggcgagtt tgtcgtgggc aacgaaatgg cacttctcta tcaacgtcat
     7561 ggtggagtcg ggctgaatgc gtcgaccggt aaggatatca cccgctatgt gatcagcctg
     7621 cctgccaatc gcttgccatt gtgggcgtcg ctggaatccg accgcatggc gcatccggtg
     7681 ttacgtgaat tctataagga gcggggtgtc gtcatggagg agcgccggct ccggaccgac
     7741 gacagtccca acggcctgct ctacgagacc tttacctcca cggccttcca ggcccatcag
     7801 tatggtgtgc cgacgatcgg ttggggctcg gatatcatgg ccctcacccc ggccgcgacg
     7861 gaagccttct tcaaaaccta ttacggcccg aacaatgcga cggttgccat tgtcggcgat
     7921 atccagccga aagaggtcat tgcgttgatc gagcagacct tcggcaagat cccggccgcg
     7981 cctcccattc cggccttggt gaccgaagag ccgccgcagc gcggcgagcg ccgggtcgaa
     8041 atcgaattcg acgcggaacc ggcactggcg atcggatacc acaaacccac catcggccac
     8101 ccggacgatt ttgtgttcga tgtgatcgat gaagtgctga ccgaaggagt gacctctcgt
     8161 ctgtacagca cgctggtgcg ggataagcgg cttgcggcgt cggtattgtc ggatacgaac
     8221 tacccgggcg ttcgtgcacc gaatctgttc gtgattgcgg caaccccgtt ggcgccccat
     8281 acgacggcgg aagtggaatc ggcgatttat gaagaactcg aacgattgaa gacggagccg
     8341 atctcggcca aagagttcga gcgggtcctg aacggacttg atgcggatct ggtgcgatcg
     8401 ttgcgctcca atagcgggct ggcctcgcag ttggcgtttt atcagacggt cgcaggcagc
     8461 tggcgctatg tgttgaacgc gcgggccagg attgccgctg tgacgccagc ggatgtgcag
     8521 cgggtggctg ctcaatattt gaccaaaccg aatcggacgg tgggggtctt ggtgaaaaaa
     8581 gcgcccgata aaaaaatggc agccgtgggt gaggtggctc gatgaggcgc ctcatgggtg
     8641 aactggaaaa aggaaaggtc atgagacaag cagcgagatg gatgtcatgt ccggtgttcg
     8701 tgctcttgtt cctcgtccag gcctctctcg tgatggccgg ggacctggta ttggacaatc
     8761 ctcgttccat gcgattcccg acggtggaat tcaatccgcc ggacgcggag cgtctcgtgc
     8821 tggacaacgg gatggtggtc tacctccttg aagatcatga attgccgctc gtgacgatca
     8881 acgcgacgct caaaaccggc agttggctgg acccggcgga caaggttggc ctcgcgggga
     8941 tgaccggcgc agcgatgcgc accggcggat cggcctcgat gtcaccggac gaggtcgatg
     9001 aagagctgga acagttagcg gctaccattt ctatcggctt cagcaaggag tccggatctg
     9061 cctcgctcga tgtcctgaag aaagacctga agcgcggagt acagattttt tccgacctgt
     9121 tgcgccggcc ggcgtttgaa cagagccggg tggagttggc gaagttgcag gcgctggaag
     9181 ggatcaggcg gcggcaggac agtcccggtt cgatcgtggg ccgtgagttc gccaaactgc
     9241 tctatgggcc cacccatccg agcgcgcgtg aaacgtcggt gcgttcgatc gaatccatca
     9301 ctcgcgaaga cctggtcgcg tttcatcagc agaccgttca tcccaacggc atcattcttg
     9361 gcgtgaccgg cgatttcgac aaggccggca tgttggcctt gctgcgcgaa gccttcgggg
     9421 attgggccaa agggactgtg cctgatgtca cgattccggc tgtggcggag tcggagttca
     9481 aaaccggggt agtgcgattc gtgaacaagg atacgtcgca aacccatttg cgagtcgggc
     9541 atctgacggt caaagaaacc catcccgatt acgtggcggt ggccatcgcc aacgacattc
     9601 tgggcggcag ttcatttcgc agccggctgt ttaacgacgt gcgtacgaag cgcgggttgg
     9661 cctattcggt cggcagcggc ctgcgagcca gtgtgtatga tgaaggcgta tggctgatgc
     9721 gcgcagagac caaactttcg tccacacagg aggtggtgaa tcgcttcgtg gcgaatatgg
     9781 agcggatgcg taatgagccg gtgacggatg cggagctgga ggaggccaaa gaagcctacg
     9841 tgaattcgtt tgtcttttcg ttcaccagcg cgtccagcat tgtcgcgcgc ctgatggatc
     9901 tcgaatatga cggcttaccg aaagactggc tccagcagat acgggataag gtggtgaagc
     9961 tgactaagga agatatccaa cgggcggcga aaatgcattt taatccagac cggttgcgga
    10021 ttctggcggt cggttccgga gacgccttgt cgaaagtgct ggccagtttc ggcgaggtga
    10081 aggagattac gctggcgccg gaaagttgag gaagggagcg cagacaggtc gaggcgtctt
    10141 tttgctcgcg caacgcgcgg cataagaatg ccctcgttgg acgcgcgcag tgaaagccgc
    10201 cccgccctgt cgtgcgctcg gcgcattgat gtgagaaaag agattgatac aatttctgca
    10261 gtgacggccg cgtggtcata tgtccaatcc acatgaaact ggatacaatc ggttatgcct
    10321 cttgaagacg agttaagcga tatcatcaag aaggcccgca cggggcagca gcgatcggta
    10381 gccgacgtgg cccgggccgc cggttttccg gaggcggacc tggccggact ggaacgcggc
    10441 cagcctgcgc gcaatcgaga gcaggtgcag gcggtgggcc gtgcgcttgg gcttcgcgca
    10501 gaacagttag cgcaggtggt ggaaggctgg accccagagg cccttccggc ctccacgcct
    10561 cacgtggaca ccattctggg ttccatcggt ggctatgagg tcaaaggcta tatcgtgcat
    10621 gatcgcggtg aagcggtaat cgtcgatact gggtacaacg ccgacgccat gttggagatg
    10681 ctctccaagc gggcattgcg ccttcgggcg atttgcctga cgcatgggca ttccgaccat
    10741 gccgatggga tcgacgccat tctgaaggct catccggtgc ctgtctatct cggtcctgac
    10801 gatctcgatc tcttgagctg gcgcccctcg caagacgtgc tgcacgtgcc caaggaagac
    10861 gggacgctgc aggttggcgg gctgaggctg cggttcatgc cgacgccagg ccatactccg
    10921 ggcggggtct gttatcgtct cgaacaggca ggagagccga tgtgttttgt tggcgatacc
    10981 ctgtttgccg gttcgatcgg ccggtcgaat ccagccggac tgtatcagac acatcttgct
    11041 tccgtccgcc agcgcgtgct ggcccttcct ccggatacac ggttgtttcc cgggcatgga
    11101 ccggccacga cggttcacga agagcgtctt cataatccgt tcgcgacgta acaccagagg
    11161 cctgtcatga accgagacga gtcggatcag aaccggtctt cgggtgaact gatatcccct
    11221 tcgttcgaga acgccgagga agacgaatgg gtggaggaag agcgaccggc attttccgcc
    11281 ttcgtgcttc ccatcatgtt gtttgccctc acgctgttta ccgtcctctg ggccggtgcc
    11341 tatcaaacga acaccaatcc tttggtcggc ccctgggatt ttcttgtcga cgaccccggc
    11401 tcgttgtgga gaggtattcc ctttgcggcg acgctgctag gcattctcgt cactcacgaa
    11461 ctgggccact atgtgttatc gcgtctgcat ggcgtgccga cctccctgcc gctgttcgtg
    11521 ccggggctgc cgcatttcgt gggcacattc ggggcgatca ttcgcatgcg cgctccgctg
    11581 accgatcggc gagcgctctt tgatatcggg gtggccggac cgattgccgg gttcgtcgtc
    11641 gcggtgatcg ccttggtgat cgggctccgg ctttcgacag tggtgccgat tcaaggtaat
    11701 tacggcatgc accttggaga gccgttgttg ctacaattcg catcctgggt cgtgatcggc
    11761 cccttagcgc cgaccgcgga tgtgattctg catccagtcg gtttcgccgc ctggttcggc
    11821 ctcttcatta cctcgctgaa cctcttgccg attggtcaac tcgacggagg tcacgtcgcc
    11881 tatgccctgc tgggagagcg tcagcgcagt gtggcggtcg cgctgattcc tatcctcatg
    11941 gtcttcggtt ggcttggctg gaaagggtgg ttcttgtggg tggggttggc tggcatgatg
    12001 ggtttggcgc atccgccggt gcgaaatcct ggccgcgatt tgggaaccgc ccgtgtactg
    12061 atcggctggc ttgccctgtt catttttgcc gtgaccttct cttgggaacc cttcatcctg
    12121 cgctgattga tgcactgtcc aaaatgcgga tgggatcagg acgagggcac tgcagactgc
    12181 gcccgctgcg gcatcatttt cgcgaagatg catcgctcgc cggctccgcc ggtgatgccc
    12241 cgcagtccgg gacaggactc gactcggcct cgggtcgttg aagaatggct ccttatcact
    12301 gaggactcgg tcaatccatt ctactttgcc ggacgtgtgc tggtctttct gttgctggtc
    12361 tcgtggggct ggcgattcat caccaccccg ctcgagacga attacaccgg cgaatccttc
    12421 ctccacctga tcaatcttcc gttccatgaa gccgggcacc tgatcttcat gcccttcggg
    12481 cgattcatga tgatcctcgg cggcagtctg ggccagatcc tcatgccgct gatctgcctg
    12541 ggcacatttc tgctgaagac gcgtgatccc ttcggcgggt cggtggcgct ctggtggacg
    12601 gcgcaaaact tcatggacgt cgccccctat atcaacgatg ctcgtgcaat ggatctcatt
    12661 ctgctcggcg ggttcaccgg caaagaggtg gatgcgcacg actggaacaa tctcctcacc
    12721 atgctggggt ggctgcagta cgatcatggt ttggcgaagc tagcctatgg cgtcggcaca
    12781 gttctgatgc tggtgtcgtt gctctggggc gcgtcgttgc tccgacggca gtatcgacga
    12841 ctggactggt agcccgctgc tgattcgcaa ctcccgttct tgaaaagccc cgagacatcg
    12901 ggcacactgc cggtcatgcc tatcgtgtgg atgcgcggcc gagtgtttgc ttctctgatc
    12961 ttcctgtggg ccggcgtgtc attgagtgcc gttcatgctg aagaggcggc ctcgcgattt
    13021 acgttggtgc tgaacggaga ggcagtgaaa gattcggcca gcggcctggt ctgggagcgg
    13081 gagccggatt acatttttga tgtgtgggac cgctccgttg cgcgatgcgc gacgaaaacc
    13141 gttggtggaa cgcagggctg gcgcgcgccg agcatcgacg aaatcaagac cctggtcgat
    13201 ctcgatcagc aggatccctc gctaccggct ggtcatccct ttcggaatat caagtctggc
    13261 atctattgga ccgccacacc ccatcccaag gatgagatcg ttgcctggca gcagagtttc
    13321 ttttccggtc agtcagtgac cgatcagaaa tcgggcacga ggcgattgtg gtgcgtgctc
    13381 ggagacgccg gcaaataacg cctctgctcg ctcgaagtca gtgtctccct tctctctacc
    13441 tcgacacatt ttcctacaac tccttagcaa gtcagtcgta ctgcgcggtt ccttcgcggc
    13501 gggtacattg cgcagcgacc aagaaggtga taaatgaaga ggtgataccc tgctcgttcc
    13561 acttgtactt tgacaactgc aagagctgac gtgtctcttg atgattcggt gagcatcgcg
    13621 atgctgcgtg cagtgagggt agtgtcgtaa tgaccaccct cctgaccgtc cttctcggct
    13681 gttacctcct cggggggctg ctcccactct gcacatcacg cccgcatctc cagaacattc
    13741 tggcgcatag tgctgccgct ttggccggcg gagctgggat tgcgctggga ctcctcgggc
    13801 ttgtggcttc ccaagcactg acgatttccg ttccctccaa cattccttac ttaacgtttg
    13861 cgctgcggct cgatccgttg gcggcgtttt tcgtcctgac catttcactg atcgggatgg
    13921 ctgcctcgat ctatgcatat ggctatgtgc gcgagttcac cggccgcgtg tcgattggcc
    13981 tgcttggctc gctgctcaat ggatttctgc tttccatgac gctggtggtc ctggccgaca
    14041 acgggttctt cttcctgatc gtctgggaa
//
LOCUS       DAOQEW010000095        14048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1230_length_14048_cov_8.951261, whole genome shotgun sequence.
ACCESSION   DAOQEW010000095 DAOQEW010000000
VERSION     DAOQEW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_14048_cov_8.951261"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..627
                     /locus_tag="PLS35_11210"
     CDS             118..627
                     /locus_tag="PLS35_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19280.1"
                     /translation="MVSSTGTKSFLLLTVGMLLAGCQLFDSSTRAAAPRSPSVMSMWE
                     LYRHCQSSGDVETVLSAAKQLQQSAADTPVVPAPDVPKSLDRFVTKQPVRTTVDPKAL
                     AASCTLQAARTSLSAGREQEAEQLLYAVVFSYPESDYTFYVAQAKVWIEELHRPGSSD
                     PVIHPISTY"
     gene            769..1755
                     /locus_tag="PLS35_11215"
     CDS             769..1755
                     /locus_tag="PLS35_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19281.1"
                     /translation="MTRPGQWRTFWRGKTCLQLVRGVMVHVWPFSIVGRLFYRLRWSK
                     PCYQIINRQARRVFDSIPQPLHPRSVEAVQALNTDGIYRSSVDALVHDADLFHELSHD
                     AQMLLAGQDIQRKIRQRYNPHDAKWYVVRAIGHRANRPSLPSSFVRFFLHPQVLSIAS
                     TYLGLQVRLNYTDVWHNIPVREGEPPISAEFWHRDHEDKRVIKLFVFLTDVDETMGPF
                     TYVRRSQVGGEFGHVYPAIPPIGRYPEQDALSQLITGTPLPSVSCVGPAGSIILCDTS
                     GLHRGGRSVTKPRIVLVGVYTSNAGMDAARYALPRSLPHEHLSPAAKFALYS"
     gene            1887..2435
                     /locus_tag="PLS35_11220"
     CDS             1887..2435
                     /locus_tag="PLS35_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNI19282.1"
                     /translation="MGERIQVSGRVVRCVGLLAAAFVAAGWLGAAQAADELPKESVLP
                     AALAGKAVQAALDACKKDGYRVSASVVDRAGVLRAMLRADGAGPHTVDSSRKKAYTAA
                     SLRRATSELADMIAKQPALQALREMNESILMVGGGLPIEIAGEVVGAIGVGGAPGTHL
                     DDACAEAGLDAIGAASKMPAAK"
     gene            2432..2896
                     /locus_tag="PLS35_11225"
     CDS             2432..2896
                     /locus_tag="PLS35_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19283.1"
                     /translation="MNLVQRRGPALLVMGLVLLLAACNSDRPEPAEADVPSAGLRLRV
                     TRVATHPFLSRYRLTLQVEGSRGCTDTAELFPDTGYAGRRNVYAQASGAITLLGQYDA
                     RVIDPAQCRIRLVEFQSVGEPSKFLGAFDVDAEKRWQFIPASMRPERPFEKH"
     gene            complement(2904..3803)
                     /locus_tag="PLS35_11230"
     CDS             complement(2904..3803)
                     /locus_tag="PLS35_11230"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI19284.1"
                     /translation="MAQAEFRIGIVGVGRMGANMARRLHDLTYQLVAVYDTEASRAET
                     LANELGCAVAPTPARVAELANTILTVVPDDAAMRHIYAPNTPDSLLRHATGRLFINCA
                     TLSPAAQMEVHRLVEQQGGHSLEACMASSIPQARHGTLYLMCGGRRDVFERATPLLKD
                     LSAHLRFIGPAGDAAKVKALVNMVMNANTAALAEGLGLASALGLDLTMLREVFSQTGA
                     NSRVLETDGEDMQQRAHDCYFSAAHAAKDSGIACSLAEQVGLELPLAKATYDQYRRLV
                     TLGKGELDKSAVAELTFKERLTS"
     gene            3931..4152
                     /locus_tag="PLS35_11235"
     CDS             3931..4152
                     /locus_tag="PLS35_11235"
                     /inference="COORDINATES: protein motif:HMM:NF023510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HNI19285.1"
                     /translation="MQQSIIGYHQDEEGHWVADLRCGHGQHVRHQPPMTSRPWVLTEE
                     GRQVFIGTELNCKKCDEGGDGFSPAEPLS"
     gene            complement(4231..5046)
                     /locus_tag="PLS35_11240"
     CDS             complement(4231..5046)
                     /locus_tag="PLS35_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19286.1"
                     /translation="MQSITPHPRLRSVFTLCWMVGGAALTLGGCVSQQTYDTARHEVK
                     TRAGELAQTQADIHSLEQEREAAHLANQRDERTLANLKSELKTIQASYDQLHKANQAK
                     LALLEHNIAALRAKHQAMLKEIGDTKRIEKRLEALTTLRDRTMGTQQSGPEAQATPVD
                     ISSPEQHMVAVITPQPSQADASSLPPIPATVPAQLPPAPAAAAAPAPATSSAAPAAVA
                     PATPNAPAKVSQAPAAAAAPPAPRPVPAAPPAEESWFSSVTGWFSSLIDWLWA"
     gene            complement(5220..6320)
                     /locus_tag="PLS35_11245"
     CDS             complement(5220..6320)
                     /locus_tag="PLS35_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNI19287.1"
                     /translation="MNSDIIQRIEIWPVDIPITDPFVVATGARVTAQNLFMRVTLKDG
                     THGVGEAAPFPEVGGEDRASCLAAAQKLAAGLIGHSAGDYETLADHLTEQAPLFPAAR
                     CGLETALLDAYCRSQRLPLWQLWGKADVRERETDITIPICDLDKTLALSRGWYAQGFR
                     LFKMKVGTDVEEDIRRLQAVHEALPGISFIGDGNQGFSREDCIHFAGRVKQFGGRLIL
                     LEQPVVRDDLEGLHAIRHLTGIPVAADESVRSLDDARRVVRMQAADYINIKIMKTGVI
                     EARRIADFTRSSGLRLMVGGMLETRIAMGCSFSLVLGLGDFDVLDLDTPLLLSTDPVI
                     GGYRYQGPRLLPWQEAGLNMQMAVPTHCLTIQ"
     gene            complement(6349..6993)
                     /locus_tag="PLS35_11250"
     CDS             complement(6349..6993)
                     /locus_tag="PLS35_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNI19288.1"
                     /translation="MDQNDRLIKTKENWAKARRGGEEREVFYEGDDRLPPGQHLVETW
                     PVLDLGQKPDIPLTEWTLTIGGAVAAPMTWTWTDFLAQPQFKDISDFHCVTSWSRYDN
                     EWEGVSFKHVAAVVQPHPSARFVLFKSYDDYTTNLPLSACLDDDVLLTYKWNGRPLTK
                     DHGGPVRMIIPKRYAWKGAKWIKEITFSEHDEKGFWEVRGYSNTALPWQNDRYG"
     gene            complement(7230..7379)
                     /locus_tag="PLS35_11255"
     CDS             complement(7230..7379)
                     /locus_tag="PLS35_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19289.1"
                     /translation="MGPLSVHDMKGGFFLIASFVLLATICAIAALGTLMRPQRWCERE
                     TRLHV"
     gene            7816..8532
                     /locus_tag="PLS35_11260"
     CDS             7816..8532
                     /locus_tag="PLS35_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HNI19290.1"
                     /translation="MTISADSSLPKARAGSGKLVLALLVGCAVAGFLYFDLGRYLSLD
                     ALKANRDQLLAFTEANYSTAVAVYVLAYCAVVGLSLPGGAIMTLAGGFLFGSVLGTLY
                     VNVGATVGATLAFLVARYLLRDWVERQFGSRLGAIQEGFSRNAFSYLLTLRLIPLFPF
                     FLVNMVSGLTRVNVGTYVAATSLGIIPGSFVFAYAGRQLGTINSLKEIVSPNVLIAFT
                     LLGLLALVPILYKRFAGHSS"
     gene            8584..10146
                     /locus_tag="PLS35_11265"
     CDS             8584..10146
                     /locus_tag="PLS35_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HNI19291.1"
                     /translation="MSVRTADSQHLVLPDDEHNRKLVGNVHPSNWVNPESDGRYNIVV
                     VGAGTAGLITAVIAASVGAKVALIERHLMGGDCLNVGCVPSKGLIRAANAWAQVRDAP
                     VFGARIPDGASFDFGAAMARMRQLRARISHVDSAHRYKSLGVDVYIGQARFVGPDTVS
                     VEGAAGNRTLAFVKAAVCTGARAAAPPIPGLDEAGYLTNETIFSLTALPARVGVIGAG
                     PIGCELAQALARFGSRVSLIEAMHGILPNEDRDAAECVQQSMIRDGVALLCCGKDLRV
                     EKVSDGKRLIVDSHGRHHDLTVDEILVGVGRAPNVQGLGLEAVGVEFDKTGVTVNDRL
                     QTTNPRIYAAGDVCSRYKFTHAADAMAQILIQNALFPHPFGLGYASMASLNMPWCTFT
                     SPEIAHVGLYEAEAVKQGLQVETYTYKFDEVDRAILDGDEEGFARLHIKAGTDTILGA
                     TIVGSHAGDVISEVSVAMKAGVGAKTIAATIHPYPTRAEVIKKTVNLWRKAHFTPRTK
                     SLLTKLFAWLRR"
     gene            10153..10890
                     /locus_tag="PLS35_11270"
     CDS             10153..10890
                     /locus_tag="PLS35_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNI19292.1"
                     /translation="MRPIGPILCLALFGATVMGEAQQPALLPVGTFSAATEGSALPDG
                     WHMLTFKKIERHTQYDVIKDQAISVVRAVSEAGASGLTKAVTIDPREYPIVRWRWKIE
                     NVLNKSDVSRKEGDDYPARLYITFAYEPDRVSFGKKLKYKAGQALFGDIPIAALNYIW
                     DSKSPVGTVVDNAFTSFAKMIVVESGARRIGSWIEEERNVYQDYLSAFGEEPPAINGI
                     AIMTDTDNTKERAVAFYGDIEFVRAPK"
     gene            complement(10993..11406)
                     /locus_tag="PLS35_11275"
     CDS             complement(10993..11406)
                     /locus_tag="PLS35_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19293.1"
                     /translation="MKINVETSWLGIAVATLATISFAVAPASAKEKDKSGKHHPPAAT
                     TSAAAAASLLGSVPEQSAAVKPGQYDSKPGVAWKTVGGTVKDIQGETYTVEDYDGSQM
                     KVRVGQGTKQLRGHKKVGDTIRAEITHGGFANSIQ"
     gene            11733..12050
                     /locus_tag="PLS35_11280"
     CDS             11733..12050
                     /locus_tag="PLS35_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19294.1"
                     /translation="MKQVIWSVAILSMCVPSLVFAQAETPRIDQRQANQERRIDQGIA
                     SGQLNEREATRLNNQQEHINNIEDKAKSDGVVTKKERARIKHAQDRTSRHIARQKHDR
                     QQR"
     gene            12284..>14048
                     /locus_tag="PLS35_11285"
     CDS             12284..>14048
                     /locus_tag="PLS35_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI19295.1"
                     /translation="MDLITTHLNADFDGLASMVAARKLYPGALLVFPGGTQESVRKFL
                     ADHDLGICRLKDVALEHVHRLILVDVQEPERIGPLKVLSSRKDLDVHIFDHHGNGEGV
                     ETSAWASKTQRHIEPVGATATLLIERLQSQQVVLTAAEATVLAMGLYEETGSLAYPTT
                     TPRDLEAAAFVLRAGADLNRVAETLQHPLDPDLIALLNDLLQSGEIYYLEGRKILVAS
                     SAYDRYTGDLAEAVQRLAELQGFDAVIVAIAMDEKVQIIGRSRRPEIDVAWIAREFGG
                     GGHAVAAAASVKGRTLVEVQEQLTRLLTERYRPTLLARDVMTTPVKSIVADATVTEAE
                     RSMTTYGVNVLPVVDTKDRYIGMISRETVQKALFHQLGDQPVGDLAGSGHYSASPDTP
                     FHDIETRMIELNQRFVPVLSDGKIVGVITRTDLLRSLHDDVLASARSKPKSFIGLESP
                     GGVRKRDLKGLLRDRLPPRVHDLLHASGVLAERLGLSLYVVGGFVRDLLLGLENLDVD
                     LVVEGDGIAFARALAKERAGRVKVHERFGTAVVLLPDGFKIDVATARTEYYEYPTALP
                     TVEQSSIKKDLYRRDFTINTLA"
BASE COUNT         2801 a         3889 c         4392 g         2966 t
ORIGIN      
        1 tcgacgacga tctgtcgtca tgatcgcgac gcggctgtca cacgtctgca ttcgtttctg
       61 taaattcctc cccgaaattc ttgacaccgt cggaaccgat ccggtaagga taggctcatg
      121 gtctcttcca ctgggacgaa gtcatttcta ttgctgacgg tcggcatgtt gttggcgggc
      181 tgccaactgt tcgactcatc gactcgcgcg gctgcaccac gatcgccgtc ggtgatgtcg
      241 atgtgggaat tgtatcggca ttgccagtcg agcggtgatg ttgaaacggt gttgtctgcg
      301 gcgaagcaac tgcagcaatc cgccgccgat acgcctgtcg tacccgctcc cgacgtgccg
      361 aagagcctgg atcgatttgt caccaagcaa ccggttcgca ccaccgtcga tccgaaagcc
      421 cttgctgctt catgcaccct gcaagcagct cggacaagcc tgagcgccgg gcgtgagcag
      481 gaagccgaac agctgctcta tgccgtggtg ttcagttatc cggaaagcga ctataccttc
      541 tatgtcgcac aggcgaaggt gtggattgag gagttgcatc gtcccggctc ttcagatcca
      601 gtgattcacc ccatttccac ctactaagcg gccgtcgatc tcgtctgctg ttgctcggcc
      661 cgatctagcg gtctctcgta catttcggag tgaggttgtg acgcgcaagc attgcggctt
      721 gtctggaaat ccgcccatgg tgcacgctca ggccgggagc gaagcagcgt gacacggcct
      781 ggacagtggc gcacgttctg gcgagggaaa acctgtctcc agctggtgcg cggcgtcatg
      841 gtgcatgtat ggccgttctc gattgtcggg aggctgtttt atcgcctacg ttggtcaaag
      901 ccttgctatc agatcatcaa tcgacaggcc cgccgggtgt ttgactcaat cccgcagccg
      961 ctgcacccgc ggagcgtcga agcggtccag gccctcaaca cggatggcat ctatcgcagc
     1021 tcggtcgacg cgctcgtgca cgatgccgac ctgttccacg agctgtcaca cgatgcacag
     1081 atgcttctgg cgggacagga cattcaacgc aagatccggc agcgatacaa tccgcacgat
     1141 gccaaatggt atgtagtgcg cgctatcggc caccgcgcca accgaccgtc gttgccctca
     1201 tccttcgtcc ggttttttct ccacccgcaa gtcctcagca tcgccagtac gtatctcggg
     1261 ctgcaggttc gtctgaacta taccgatgtc tggcataaca tccccgtccg cgagggcgaa
     1321 ccgcccatct ccgccgaatt ctggcatcgc gatcatgagg ataagcgggt catcaagctg
     1381 tttgtgtttc tgaccgatgt cgacgagacc atgggtccgt tcacctacgt aagacgatcg
     1441 caggtggggg gcgagttcgg ccatgtctat cccgccatcc ctcccattgg tcgttatccc
     1501 gagcaggatg ccctgagtca actgatcacc ggcaccccac tgccgtcggt gtcctgtgtc
     1561 gggccggccg gaagtatcat tttgtgtgat accagtggcc tccatcgcgg tggccgatcg
     1621 gtgacgaaac cgagaatcgt cctggtcgga gtgtacacgt ccaacgccgg gatggatgcc
     1681 gcacgctatg cccttcctcg ctctctcccc cacgaacact tgtcgccggc cgcaaagttc
     1741 gccctctatt cgtaacccgt cacgcctctg tgtcgaatcc gactacggga acccgagtat
     1801 tgacagagcg gtgtgatggc cgtaggctcg cgcggtgcag tgtggatgat tcatgacgga
     1861 cgaagaccga atgaaggagg catcagatgg gagagcgaat acaggtgtcc gggcgggtgg
     1921 tgagatgtgt cggcctcctt gctgcggcgt tcgtcgccgc cggatggctg ggagcggcgc
     1981 aggcggcgga tgaattgccg aaagagtcgg tgttgccggc agccctcgcc ggaaaggccg
     2041 tgcaggcggc cctcgacgcc tgtaaaaagg atggctatcg ggtcagcgca tcagtcgtag
     2101 atcgtgccgg tgtgcttcgg gccatgctgc gggctgatgg tgccggtccc cataccgtcg
     2161 acagcagccg aaagaaggcc tacacggcag ccagtttgcg ccgtgcgacc agtgagttgg
     2221 cggacatgat cgccaagcaa ccggcgctgc aggcgttgcg agaaatgaac gaaagcattt
     2281 tgatggtggg aggcggtttg cccattgaaa ttgccggtga agtggtcggc gcgatcgggg
     2341 tcggcggcgc gccgggaacg cacctggatg atgcctgtgc cgaagccggt cttgatgcca
     2401 tcggcgcagc ctcgaagatg cctgccgcga aatgaacctg gtgcagcgac gaggaccggc
     2461 cttgttggtg atggggctgg tcctcctcct ggcagcttgt aacagtgatc gtccggaacc
     2521 ggctgaggcg gatgtgccct cggcagggtt gcgcttgcgg gtgacacgcg ttgccacaca
     2581 tccctttttg tcccgctacc gtttaacgct gcaggtagaa ggatctcgcg gctgtaccga
     2641 tacggcggag ttatttcctg atacgggata tgccggcagg cgcaatgtgt atgcgcaggc
     2701 gtcgggggcg attactctgc tggggcagta tgatgcccga gtgattgacc ccgcgcagtg
     2761 cagaattcga ctggtggagt ttcagtcagt aggggagcca tcgaagtttt taggcgcgtt
     2821 cgatgtggat gctgagaaac gttggcaatt tatccctgcc tcaatgaggc ccgagcgccc
     2881 gttcgaaaag cactaagtga tcctcaagaa gtcagccgct ctttgaaggt cagttctgcg
     2941 actgccgact tatccaattc accctttccg agcgtcacca gccggcggta ttgatcatag
     3001 gtggccttcg caagcggaag ttccagcccg acttgttcgg ccaaactgca ggcaataccc
     3061 gaatctttcg cagcatgggc ggcggaaaag taacaatcat gcgcgcgctg ctgcatgtct
     3121 tcgccatccg tttccagaac gcgggagttg gcgccggtct gagaaaagac ttctcgcagc
     3181 atcgtcagat ctaatcccaa ggcggacgct aatccgaggc cttccgccag ggcggcggta
     3241 ttggcgttca tgaccatgtt caccagcgct ttcaccttgg cggcgtcgcc ggcgggcccg
     3301 ataaagcgca agtgcgcgct cagatccttg agtaacgggg tcgcgcgttc gaacacgtcc
     3361 cggcgcccgc cgcacatgag atagagcgtg ccatggcggg cctggggaat actgctcgcc
     3421 atgcaggctt cgaggctgtg gccgccctgt tgttccacca gcctgtgcac ttccatctgg
     3481 gcagcgggag agagcgttgc gcaattgata aagagacggc cagtcgcatg ccgtaacaaa
     3541 ctgtccggcg tatttggcgc atagatgtgg cgcatggctg catcatccgg gaccactgtc
     3601 aggatcgtgt tggccagttc ggcgaccctg gctggagttg gagcaaccgc acagccgagc
     3661 tcgttggcga gtgtttcggc gcgtgatgcc tcggtatcgt acacggcgac aagctgatag
     3721 gtgaggtcgt ggaggcgccg ggccatgttg gctcccatcc gtcctacccc gacgatgccg
     3781 atacgaaact ccgcctgtgc catccgcaac ctcctggaaa acatggtact ctgactggcg
     3841 tggcagtgta ccacagtcta ttcaggcccc ggtatcacgg catcggctgt gaggcccggg
     3901 ggcgttcgga taacgacgag ggagtgtgtg atgcaacaat cgattatcgg atatcaccag
     3961 gatgaggagg ggcattgggt ggccgacctg cgctgcgggc acggccagca cgtgcgtcac
     4021 cagccgccga tgacgagtcg tccgtgggta ctgaccgaag aggggcggca ggtatttatc
     4081 ggcactgaat taaattgcaa gaagtgcgat gaggggggag atgggttctc gccggcagag
     4141 ccgctttcgt aagcgccgtg gagagaaagc gatacctgtg gcccggacca tcacaaggtg
     4201 gtgaggtccg agccgattcg catatctatc ttaggcccag agccaatcga tcaaagaaga
     4261 aaaccagccg gtcacgctgg aaaaccagga ttcctccgcg gggggtgctg cagggaccgg
     4321 ccgcggggca ggcggagcag cggcagcagc cggggcctgg ctgacctttg ccggtgcatt
     4381 cggcgtagcg ggtgcaacag ccgctggtgc ggctgaggaa gtcgccggtg ctggagccgc
     4441 ggctgccgct ggagcagggg gaagttgggc cgggacggtc gccgggatcg gcggcagtga
     4501 ggatgcgtca gcctgcgagg gctgcggtgt gatcaccgcg accatgtgtt gctccggtga
     4561 actgatatcg accggcgtcg cctgtgcttc cggcccggat tgctgcgtgc ccatcgtccg
     4621 atcgcgcaac gtggtcaggg cttccagccg tttctcaatc cgcttggtat cgccgatctc
     4681 cttcagcatg gcctgatgct tggcacgtaa ggcggcaata ttgtgctcaa gcagtgccag
     4741 tttggcttga ttggccttgt gaagttgatc gtaggaagct tgaatggttt tcagctcgct
     4801 cttcaggttg gcaagcgttc gctcatcgcg ctgattagcg aggtgtgctg cctctcgttc
     4861 ctgttccagg ctgtgaatat cggcctgagt ctgcgccagc tcgcccgccc gcgtttttac
     4921 ttcatgcctg gcagtgtcgt aggtctgctg cgagacgcag cctcccagcg tcaatgccgc
     4981 gcctccgacc atccagcaga gcgtgaagac agatctgagc cgcgggtgtg gggtgattga
     5041 ttgcatggga cgatcctccc ggatacgaac ggtggcgata ggtgcaaaag gtggtcccct
     5101 cagcggagcc acggtgcctg aggttaattt ttcagaaaag tgaaaaaacg gcaaaatatt
     5161 tcgggaaacc tgcaggcagc ggtagggaga gtctgagcga cggcgaattg agcgtccggt
     5221 tattgaatag tgagacagtg ggtgggaacg gccatctgca tgttgagtcc cgcctcctgc
     5281 caggggagaa ggcggggtcc ctggtagcga tacccgccga tgacgggatc ggtcgaaagc
     5341 aggagcggtg tgtcgagatc cagcacgtcg aaatcgccca gccccaacac caggctgaac
     5401 gaacagccca tcgcgatgcg cgtttcgagc atgccgccca ccatgagtcg aaggccggag
     5461 gagcgggtga agtctgcgat gcgccgtgct tcgattaccc ccgttttcat gatcttgata
     5521 ttgatatagt cggcggcctg catcctgacc acgcggcgcg catcgtccag tgatcggacg
     5581 gattcgtcag ctgcgacggg aatgcccgtg agatgccgaa tggcgtgaag tccttcgagg
     5641 tcgtcacgaa ccaccggttg ttccagaagg atcaggcgcc ctccgaactg tttcactcgg
     5701 ccggcgaaat ggatgcagtc ctcgcgagaa aagccctgat tgccatcacc gataaagctg
     5761 atgcccggaa gcgcctcatg cacagcctgt aggcggcgta tgtcttcctc gacgtcggtt
     5821 ccgaccttca tcttgaacag gcggaagcct tgcgcatacc agccgcgcga gagggccagc
     5881 gttttgtcga ggtcgcagat cggaatggtg atgtcggttt cacgctcccg gacgtcggct
     5941 ttcccccaca gttgccacag cgggaggcgc tgagaccggc aataggcgtc gagaagggcc
     6001 gtttccaggc cgcatcgagc ggcgggaaag agcggagcct gttcagtcag gtggtctgca
     6061 agggtttcgt agtctccggc agagtgtccg atcagtccag cggctaattt ctgggcggca
     6121 gcaaggcagg aggctcgatc ctcgcctccc acttcaggaa acggcgcggc ctctccgacg
     6181 ccgtgagtcc cgtccttcaa ggtcacgcgc atgaagaggt tttgcgccgt cacacgcgcg
     6241 cctgtggcga cgacaaaggg atcggtgatc ggaatatcga ccggccagat ctcgatgcgc
     6301 tgtatgatgt cggaattcac caggggcgtc gtggctgcag gtggaggctc atccgtagcg
     6361 atcgttttgc cagggaaggg cggtattgga gtagccgcgc acttcccaga aacccttctc
     6421 gtcgtgctcc gagaaggtaa tttccttgat ccatttggcg cctttccagg cgtagcgctt
     6481 cgggataatc atgcgcaccg gaccaccgtg gtccttcgtg agcggccgac cgttccattt
     6541 ataggtgagc agcacgtcat cgtccagaca ggcgctcagc ggtaaattgg tcgtgtagtc
     6601 gtcataggac ttgaacagca cgaagcgggc ggagggatgc ggctggacca cggcggccac
     6661 atgcttgaag ctcacccctt cccattcatt gtcataccgg ctccaggagg tcacgcaatg
     6721 aaagtccgag atatctttga actgcggttg cgccagaaag tctgtccagg tccaggtcat
     6781 cggggccgcg actgcgcctc cgatggtgag cgtccattcg gtaagcggaa tatcgggctt
     6841 ctggccaagg tcgagcaccg gccatgtctc aacgaggtgc tggcctggcg ggagccggtc
     6901 gtcgccttca taaaacacct cacgctcttc tccccctcgc cgggctttgg cccagttctc
     6961 cttggttttg atcaatcgat cattctgatc cattccggcc tccctgttca cagagtagga
     7021 caagatgccc tgttcttaca agggttgaag cgagcaatga caaagggcga ggacacatgc
     7081 ggaaggcatg aaagggccca tgattctgcg ccaatgtgcg gtttagaagg attctcaatg
     7141 ctggagcagg agatacattg cgcacagaga gggaaggccg cagggggcgt cgacgtcagg
     7201 cagcctcaaa cgggggccga ctgggaacct tagacgtgca gtctggtctc gcgttcacac
     7261 cagcgttgcg ggcgcatcaa cgtgcccagt gccgcaatgg cgcaaatcgt ggccagtaac
     7321 acaaagctgg cgatgaggaa aaatccaccc ttcatatcgt gcaccgagag cggtcccatg
     7381 acgtcacccc cttcatatgc cctaattgta ccacttcact ccgtgcgcca gccgcagcca
     7441 caacgccatg attcttcagg tgaatttttt ctgagacggc aatggcaatg gggacagggg
     7501 cgcatccaca cgtgtaaagc ggtttcgtaa gcggggtgcg aggtacgggt ctgctgtgga
     7561 accgaatgtg ttgtcggcat ggcagcgtcc aggacatcaa tgatcgcgta cacggacgac
     7621 attacctcca ctcgcctccc ggcttcaaga gttctttcgg aggttgccgc cctgcacttg
     7681 agggtaccga tgaacgctgg gagcaaaaaa atgcagaagc gaggaaaaag aggccgcagc
     7741 atggacgacg aggacgtgat ctgattgaca caggagcagg tagacacgat gataccgccg
     7801 ggagtaccga acacgatgac gatttcagcc gattcttctc ttcccaaggc gcgcgccggt
     7861 agcggcaaac ttgtgttggc gttactcgtg ggctgtgccg tggcgggatt tctttatttc
     7921 gatctggggc gctacctttc cctggatgcg ttgaaggcca atcgcgatca gctgcttgca
     7981 ttcacggaag ccaactattc gacggccgtt gcggtgtatg tattggccta ttgcgcggtg
     8041 gtcgggctgt cattgccggg tggcgcgatt atgaccctcg ccggaggatt tctcttcggg
     8101 agcgtgctgg gaacgctgta cgtgaatgtc ggcgccacgg tcggcgccac cctggcattt
     8161 ctggttgcgc ggtatttgct gcgagactgg gtggagcggc aattcggtag ccgactcgga
     8221 gctatccagg aggggttctc tcggaatgcg ttcagctacc tgctgactct gcgattgatc
     8281 ccgctctttc cgtttttttt agtcaacatg gtgtcgggat tgacccgggt gaacgtcggc
     8341 acctatgtgg cggcgacatc cctcggtatt attccgggaa gttttgtgtt tgcctatgcg
     8401 gggcgccaac tgggcaccat caattcgctc aaggaaatcg tgtcccccaa tgtattgatc
     8461 gcgtttacgc tgttaggatt actcgcgctg gtccccatcc tctacaagcg gtttgccggt
     8521 cactcgagct gaacagagag agacgcctct ggcgagggcc ttgcgtagag aaaggtcgtg
     8581 tagatgtctg taaggactgc cgatagtcag cacctcgttc tgccggatga cgagcacaac
     8641 cgcaaactgg tgggcaatgt gcatccatcc aattgggtga atcctgaatc ggacggtcga
     8701 tataacatcg tcgtcgttgg ggcgggaacg gcaggattaa ttacggcagt gattgccgcg
     8761 agcgtcggcg cgaaggtggc gcttatcgag cggcacttga tgggtggcga ttgtctcaat
     8821 gtcggctgcg tgccctccaa gggattgatt cgcgccgcaa acgcctgggc acaggtgcgc
     8881 gatgcgccgg tattcggcgc ccgcattccg gatggggcga gcttcgattt cggcgcggca
     8941 atggccagaa tgaggcagct gcgagctaga atcagccatg tggattctgc acaccggtat
     9001 aaatcgctgg gcgtggatgt ctatatcggc caggcccggt ttgtcggccc agatacggtg
     9061 agcgtggaag gcgcggcagg caaccggact ttagccttcg tcaaggcggc tgtgtgtaca
     9121 ggggcaaggg ccgcagctcc tcccatcccc ggcctggacg aggcgggcta tctcaccaat
     9181 gaaacgattt tttcgctcac ggcgctcccc gcgcgcgtcg gcgtcatcgg ggccggcccg
     9241 atcgggtgcg aattggcgca ggctttggca cgattcggca gccgagtatc gctgattgag
     9301 gcgatgcacg gcattctccc gaatgaggat cgagacgcgg cggaatgcgt ccaacagtcg
     9361 atgatccgtg acggtgtggc cctgctctgt tgcgggaagg atctccgggt ggagaaggtc
     9421 tcggacggca agcggcttat cgtcgattct catggacgac accatgacct gacggtcgat
     9481 gaaatcctgg taggggtagg ccgtgcgccc aacgtacaag gcttgggatt agaggccgtc
     9541 ggcgttgaat tcgataagac cggcgtcaca gtgaacgatc gcctgcagac gacgaacccg
     9601 cgtatctacg ctgccgggga tgtctgctcg cgctataagt tcacccatgc cgccgatgcg
     9661 atggcgcaga tcctcattca aaacgcgctc tttccccatc ccttcgggtt gggatatgcg
     9721 agcatggcgt cgctcaacat gccctggtgc accttcacct ctccggaaat cgcccatgtc
     9781 ggtttgtacg aagcggaggc agtgaaacag ggactgcagg tcgaaaccta cacctataag
     9841 tttgatgagg tcgatcgagc cattctggat ggtgatgagg agggttttgc ccgtcttcat
     9901 atcaaagcgg gtaccgatac gattctcggg gccacaatcg tcgggtcgca cgcaggggac
     9961 gtgatcagtg aagtctctgt ggcgatgaaa gccggcgtcg gcgccaagac gattgccgca
    10021 accatccatc cctatccgac acgtgcggaa gtcatcaaga aaacggtcaa tctttggcgc
    10081 aaggcgcatt tcaccccgcg caccaagtca ctgctgacca agttatttgc ctggctgagg
    10141 cgatgaatac ctatgcggcc catcggccca atcctctgtc tcgcattatt cggcgcaacg
    10201 gtgatggggg aggcgcagca gccggccctg ttgcccgtgg gaacgttttc cgccgcaaca
    10261 gagggatcag cattgccgga tggctggcac atgctgactt tcaagaaaat cgagcggcat
    10321 acgcaatatg acgtcatcaa agaccaggcc atatctgttg tcagagcggt gagcgaggct
    10381 ggcgcttccg gactgaccaa agccgtgacg atcgacccgc gtgaatatcc gattgtccgc
    10441 tggcgttgga agatcgagaa tgtgttgaac aagagcgacg tgagccggaa agaaggcgac
    10501 gactatccgg cgcggttgta cattaccttc gcgtatgagc cggatagggt gagtttcgga
    10561 aaaaaactca aatacaaggc cggccaggcg ctattcggcg acattcccat cgccgcgctc
    10621 aactacatct gggacagcaa gagcccggtg ggaaccgtgg tggacaatgc gttcacgagt
    10681 tttgccaaga tgattgtcgt ggagagtggg gctcgacgga tcggttcctg gatcgaagag
    10741 gagcggaacg tctatcagga ttatctctcg gcgttcgggg aggaaccgcc cgcgatcaac
    10801 ggcattgcga tcatgacgga tacggataat acgaaagaac gcgccgtggc cttttacggt
    10861 gatatcgagt ttgttcgcgc tccgaaatag ctgacgtagc aacggatgtg agggatgaag
    10921 gagaggccga cctgtgcagg gtcggcctct tcggacaccg gcgagctgag gcgggttcgc
    10981 aatgtcagcg ggttactgaa tggaattggc gaagccgccg tgagtaatct cagcccgaat
    11041 ggtgtcgccc acctttttgt gacctcgaag ttgtttggtg ccttgcccca cacggacctt
    11101 catttgactg ccgtcgtagt cctcgaccgt ataggtttct ccctgaatat ctttgacggt
    11161 accgcccacg gttttccagg ccacgcccgg tttcgagtcg tattggccag gcttgacggc
    11221 agccgattgc tcaggcacgg atcctaaaag agacgccgcg gcagctgcgg atgtggtcgc
    11281 ggccggcggg tgatgtttgc ccgacttgtc tttttctttt gctgatgccg gtgcaaccgc
    11341 gaagctgatg gtggcgaggg tcgccacggc gatgcccagc catgaagttt caacgttaat
    11401 cttcataaga acctccttca gtgatgagga ctgcgggtcg gcgattgact gagcaagccc
    11461 catgcctccg agatccgggg gtgattcgct cggatttgtg ccggttgtgt ccggttggcg
    11521 tgataagaca ggaccggcag gagaggaaaa cgccaacatc tgttcaattt tgcccagcca
    11581 ttctccggat ccttcctgac ttctgctgtt tcgctgggac acgcagttct ccgtatagcg
    11641 ttctcggaaa tcaaaaactc gttgtcaacg aaccagccgc cttgtacgtt gaaatgaacg
    11701 acagggcttt cgcgataacc aagggaggat gtatgaagca ggtgatttgg agtgtggcta
    11761 tactgtcgat gtgtgtcccg tcattggtct tcgcgcaggc ggagacccca agaatcgatc
    11821 agcgacaggc gaatcaggag cgacggattg atcaaggcat tgccagcggt caactcaatg
    11881 aacgtgaggc cactcgcttg aacaatcagc aggaacacat taacaacatc gaggacaagg
    11941 ccaagtccga cggggtggtg acgaagaaag aacgcgccag gatcaagcat gcccaggatc
    12001 gtacgtcgcg ccacatcgct cgccagaagc acgaccgcca gcagcgttaa tgtttccatc
    12061 cggaccgact tcgtcagaaa ctcagcatgt cggtccgggg cacttggtcg gtctctgcgc
    12121 tcgtgcttgt tgtcgggtgc cttccatgcc gtccacaggt catactgtgg gcggcatggt
    12181 tgtatctgac ctcaattgtc atagccgttc cagtcggcac ccggtccctg gatttcacac
    12241 atcgcgcctg aatcgattca cttgatcctg ctagaatgcc ggcatggatc tcatcaccac
    12301 tcacctgaat gcagactttg acggattggc ctccatggtg gcggccagga aattgtatcc
    12361 cggtgctctt ctggtctttc ctgggggcac tcaggaatcc gtccgaaagt ttctagccga
    12421 tcacgacctg ggcatctgcc gcctcaagga tgtggcattg gagcatgtgc atcgattgat
    12481 cctggtcgat gttcaagagc cggagcgcat cggtcccttg aaggtgctga gttcgcgcaa
    12541 ggacctggac gtccatatat ttgaccatca tggaaacggc gaaggggtag agacgtcggc
    12601 ctgggcgtca aagacacagc gccatatcga gccggtggga gccactgcaa cgctgctgat
    12661 cgagcgcctg caatcgcagc aggtcgtcct cactgctgct gaagccaccg tgctggccat
    12721 gggcctgtat gaggaaacag gctcgctggc ctatcccacg acgactccgc gcgatctgga
    12781 agccgcggcg tttgtcctcc gtgccggcgc cgatttgaac cgggtcgcag agacgctgca
    12841 gcatccgctt gatccggatc tcattgccct gctgaacgat ctgcttcaat caggggagat
    12901 ctactactta gaaggccgaa aaatcctcgt cgcttcgagt gcatacgatc gatatacggg
    12961 agaccttgcc gaggcggttc agcggctcgc ggaactgcaa ggatttgatg cggtcatcgt
    13021 ggccatcgcg atggacgaga aggttcagat tatcggacgg agccgcaggc cggagatcga
    13081 tgtggcgtgg attgcgcgcg agtttggcgg cggcggacat gccgtggcgg ccgctgccag
    13141 tgttaaaggc cgtacgctcg tggaagtgca ggaacaactg actcgtctgc tgaccgaacg
    13201 gtaccgcccg acgttgttag cccgggatgt gatgaccacg cccgtcaagt cgattgtcgc
    13261 ggatgccacc gtgacggagg cggaacggtc catgaccacc tacggtgtca acgtgctgcc
    13321 ggtggttgat acgaaagatc gatacatcgg catgatttca cgggaaaccg ttcagaaggc
    13381 cctgtttcat caactcggag atcagccggt aggagatctg gccggcagcg gacactatag
    13441 cgcatcgccc gacacgccgt tccatgacat cgagacacgg atgatcgaat tgaatcagcg
    13501 tttcgtaccg gtgttgtccg acggcaagat agtgggtgtc atcacgagaa ccgacctgct
    13561 ccgtagcttg catgacgatg tgctggcatc tgcgcgcagc aaaccgaagt cattcatagg
    13621 actggagtca cccggaggag tgcggaagcg ggatctgaaa ggactgctgc gggatcggtt
    13681 gcctcctcgc gtccatgact tgctgcacgc atcaggtgta ttggccgaac gactgggctt
    13741 gtcactctac gtggtcggag gattcgtccg cgatctgttg ctcggtctcg agaatctcga
    13801 tgtggacctg gtcgtcgagg gggatggcat cgcgtttgcg cgcgccctcg cgaaggagcg
    13861 tgccggtcgc gtcaaggtgc atgaacggtt tggcacggcc gtggtactgc tgccggacgg
    13921 gtttaaaatc gatgtcgcaa cagcccgtac cgagtactac gagtatccga cggccttgcc
    13981 gactgtggag caaagttcca tcaagaagga tctttaccgg cgggacttca ccatcaatac
    14041 gttggcgg
//
LOCUS       DAOQEW010000096        13921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1249_length_13921_cov_8.546372, whole genome shotgun sequence.
ACCESSION   DAOQEW010000096 DAOQEW010000000
VERSION     DAOQEW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_13921_cov_8.546372"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLS35_11290"
     CDS             complement(<1..1044)
                     /locus_tag="PLS35_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNI19296.1"
                     /translation="MIKVADFIINTLAERGIDKMFVVYGAANGDLIDAFTRTAATQYV
                     AVMHEQAGGFAAEAYAKVKGIPGVAIATSGPGGMNLLTSMGNCFYDSIPCVFLTGQIN
                     SRFLRPDPSIRQIGFQETDIVAMASPVTKYAKMILNPEDVRFELEKALWMCQEGRPGP
                     VLLDIPLNVQKAMVDAKQLTGFEPPAAMSFDLTVVDQQIARFISDLQQAERPVILVGG
                     GVRLADAQDDVRALGRRLNVPCFPTWNALDVISSDYENYGGRVGTYGGAGRNFGIQNS
                     DLLLSIGSRISGRITGGNVQSFARQAKKYLVEIDPAMVQRKFQQVPFDVNILCDAKAF
                     TQRVLIALDRRSKP"
     gene            complement(1069..2577)
                     /locus_tag="PLS35_11295"
     CDS             complement(1069..2577)
                     /locus_tag="PLS35_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI19297.1"
                     /translation="MSKPLDVLLVTPPSRVQVYQELSRDFAAIEPPVWSGLIATYLRQ
                     HSCSVAILDAEAQGLTHQQTAEQIAAIAPRLAVFVIYGQQPSASTQCMPAGRKVCEIL
                     NTLADIPTLVMGTHPSALPKRTLLEEPYTYVCQGEGPATILGLVVALRAPQHSLREIP
                     GLWHLEEGHAVGNVPAPLFSNLDRDLPGQAWDLLDMSRYRAHNWHCFGNPDARTPYAS
                     LQTSLGCPFTCSFCCINAPFTTPMLRVWSPDNVIGQIDRLVREYGVSNIKIPDEMFVL
                     NRRHVMGICDRIIERGYRLNIWAYARVDTVQDEVLEKLARAGFTWLGLGIESGSQHVR
                     DGVEKGRFGEREIVATVERIRAHGIHVAANYIFGLPDDTLESMRATLDLALTLNTEWA
                     NFYCAMAYPGSALYGLAKQHHWTLPDDQGGPGWIGYSQHAYECLPLPTDSLTATQVLD
                     FRDRAFQEYFGHPSYLAMLQRTFGSHVVAHVAEMCRHQVRRRHHDQASHQAA"
     gene            complement(2561..3781)
                     /locus_tag="PLS35_11300"
     CDS             complement(2561..3781)
                     /locus_tag="PLS35_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNI19298.1"
                     /translation="MTQQRASMPQFKTRPAFSSRGERLIGQEMFRVLDQAQALERQGH
                     RIYHLELGNPRVAPPSQILEATIGELQAKRVGYAPMGGLQELRSAVVERYASLSGKPI
                     AASQVVISPANMLIHQFLDITCNPGERIVLFTPAFPSYWAAAAHLGLDVVPVPLPARD
                     GFSLSRPSIDAALAAKPRAIIVNSANNPTGAVYEPAILHELVARCGQAGIWLLSDETY
                     ADLTFAGSHVTLAAPQAGHVVAMSSFSKVLSVPGFRTGYALAHEAVVAKIALSNSTLY
                     SCLPGFTQSGCLAGLSVLDAYAVEVRARNRRLIATCHDRITHDGVLQCRRPDAAFYLF
                     VDISRTGLDDMTFCRRLLEEEHTAVTPGRCFGDAYASCIRLAVCGQEEDVLEGVSRTI
                     AFAKKLGGCHVQAA"
     gene            complement(3778..4542)
                     /locus_tag="PLS35_11305"
     CDS             complement(3778..4542)
                     /locus_tag="PLS35_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNI19299.1"
                     /translation="MAQFADCDVIILCGGFGTRLQSVVSDRPKPMAEIHGRPFVSLLV
                     EHFLRHGARRFIFSTGHFGEMIEDWFLRHRGAYETLFVRDPVPLGTGGALAHAMPLVR
                     SNPFLVLNGDSFCEIDPERLLRFHTRKRAGATIAVTHDESREDTGAVALGEGDRVLSM
                     VEKPRTRTANYHNAGVYLFDRAVTTLFPDMPVWSLERDLLPRLVTHQCYGFVTASPLH
                     DIGTPERLAHFREIWKDTSAYFPTSLLHQTQGSIAR"
     gene            complement(4542..5612)
                     /locus_tag="PLS35_11310"
     CDS             complement(4542..5612)
                     /locus_tag="PLS35_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HNI19300.1"
                     /translation="MIISRTPFRISFFGGGTDYPVWFREHGGAVLATTIDKYCYISCR
                     QLPPFFEHRTRIAYSRIELVNNNQEIEHPAVRGVLKYLNIDDGLEIHHDGDLPARTGL
                     GSSSSFTVGLLHTLYALQHIMPSKAQLAQAAIHVEQDVLGEAVGCQDQVLAAHGGLCK
                     ATFFQNGDIGHAPIIMRPERLAAFQNHLQLYFTGFSRIASEVAREQIDRTNQRRSELS
                     TMLQMVDEGVSILTGDRDLADFGSMLHEAWMLKRRLTSRITTPAIDAIYAAAREAGAL
                     GGKLLGAGGGGFMLLFARPEDHERIRLALPGLLQVPFKFEGLGTQIVFYQEDQLMLDD
                     VWAQSSAQTAARLKATLIGKAA"
     gene            complement(5643..6590)
                     /locus_tag="PLS35_11315"
     CDS             complement(5643..6590)
                     /locus_tag="PLS35_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI19301.1"
                     /translation="MTTGSTHVLITGGAGYLGSVLTRRLLDRGYAVTVLDNFMYRQNS
                     LMDCCAEEKFQVVRGDCRDERLVTDLLRKADIIIPLAALVGAPLCDRDRVGAYTVNFE
                     AVQLLCKLSSPRQQIIFPVTNSGYGIGQPGVPCTEDSPLRPISLYGETKVKAERVVLD
                     RGNAITLRLATVFGVSPRMRMDLLVNDFVWRAVHDRAVVVFEGHCKRNYIHIRDVVRT
                     FLHAIDHFDDMKNRPYNVGLDDANLSKLELCREIQRVLPQFVSFEAPIGEDPDKRDYI
                     VSNQRLLETGFKPEWSLERGIRELIKCYTVVKAGQYANV"
     gene            complement(6643..7836)
                     /locus_tag="PLS35_11320"
     CDS             complement(6643..7836)
                     /locus_tag="PLS35_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNI19302.1"
                     /translation="MTLHAHSAKLNPRWCLAQDTIDRQDIDHLIDWLRTYPRLTKGAV
                     TVDFEQRWSQWLGRPYSVHCNSGSSANLLMYYALLRSGKLRNTNVIVPSVGWVTSIAP
                     AIQFGFTPFMCEADPDTFGLDLNHLEDLLKRHHAQTVLLVQVLGVPHRMRELQTLKDR
                     YGFYLLEDACAAIGAEYDGKKVGTFGDMASFSFYFGHQMSTIEGGMVSSSDKELTNLL
                     LMLRSHGWSKDVDQARHQELVGQYQVDDFHSPFVFYEPGFNLRSTDLNAFIGLEQLRK
                     LDWMTGRRQANHDRYLEQLGTRFYTQRPPKGSKVASISFGLLAESPEQRKRIVRALVA
                     EGVETRIFSAGNLGLHPFWMNRYGKASFPVADRVHHCGFFLPNHASMTDQDVLHISRI
                     VLEAA"
     gene            complement(8034..8747)
                     /locus_tag="PLS35_11325"
     CDS             complement(8034..8747)
                     /locus_tag="PLS35_11325"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HNI19303.1"
                     /translation="MSDLIVHRHPASFLEVGLSLQLSLPAEGTGAQYGATVLGWKERS
                     WIVCEWPFHFGQPISCAAGTPCLLRYIYNGKMIGYRTDVLHAQLQPFPFLLLAFPATL
                     DEVPLRKHGRVTAQEPIVLLQADQNLRNQPTGSPCRIGGLLTDLSASGCAVLVQRPMQ
                     DFFPGMVIRAEFEIVGTGHVNNLTGVVRNVSMQADGTHLGLEFRFDGKETIEYRGWGG
                     SVRKALESFVLQKHSFESA"
     gene            8954..10204
                     /locus_tag="PLS35_11330"
     CDS             8954..10204
                     /locus_tag="PLS35_11330"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNI19304.1"
                     /translation="MASTKRIPIDQLTVGMFIAGLDQPWYRTPFLLHKWLVSNPDDIV
                     QLKRHGIQIVTIDTDRGLDVGAAPVAAPESTAPVPEPSPASAESVDASTNGHSSHAAA
                     AAATYREAMEAVERVFTDLEAGQPPKIAALKPVVSKLLKQIIEQPEAMMIQFCLDKMR
                     RFDGTLANHGMDVCVLTLILAVENSCTESDMESLGLGALLHDIGFVRLPRNLYRKTSA
                     LTEQEQVLMRQHPQLAATVLSKIDSLPETVSQIIAQHHEFQDGSGFPKLVKSGGLSPL
                     AQLMALADTYDDLVTSRYGRAPLLPHDAIRQLFVLGAKGRYDKNLVEVAIKMLGVYPL
                     GSLIKLNTNECAVVIGLNHEDRLRPRIRIIRGPDGEIQNPPIDSDLQNQAADQPVRTI
                     LRALDPKQEQIDLPQYLDVAVGGA"
     gene            10207..13047
                     /locus_tag="PLS35_11335"
     CDS             10207..13047
                     /locus_tag="PLS35_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19305.1"
                     /translation="MSRPSHSRSHHPQPPSGFTPSPEMMGLLWEAVPLGICLLAPDHT
                     IVFANRMAARLLGRSPADCAGKRCSELTGQPLELNDSLHSTGVWKFQSGAAAASDETT
                     DMNATDAPALTVIEWEQLRLSGIPSVSTLLTLRDISRECELEQDRDRLATVAEESPCP
                     IVEIDRNGNILYVNPAMVEVLCRFGYDPNGKPDILPDNLPALVATCLLEGRSLTSQEV
                     VRGEACYSWTLCPVPSNQLVRAYGIDLSEVHATHRALNATADHLRESNRQLDQALQQA
                     QAAARAKSSFLAMITHELRTPMNGVIGMASLLLDTSLTEEQRSFTQTIQQCGEAQLSL
                     INDVLECSKIEAGKLELETLDFQLRTTVEDVLSQFAERAQRKGLEITGLVHAAVPNAL
                     RGDPGRLRQVLTNFVGNAVKFTEQGEVTLQAFLESDTPAGVMIKFEVTDSGIGISEEV
                     QGRLFQAFTQADSSTTRKYGGTGLGLAISKQLVELMGGTVGLRSKPGEGTTFWCTALF
                     QKQAVCSPAIVPSAELSGRRILIVDDNESNRTILHHLVSGWGMHDGQARNATEAMHML
                     EQAAANGEPYDAAVLDMLMPGKDGLQLAQDIRAHQHGAGVRLVVLTSLIQPGHAERAR
                     RAGFTAYLTKPVRHDQLQGCLRVVFGIQHKAATASAATGKGQPISPTLITRHTLAEQS
                     PRPRILVAEDNVVNQKLAVRMLDRLGYQPDVVSNGQEAVTAFERESYAAIVMDCQMPT
                     MDGYEATRLIRAQEERPDASRTRAHIPIIALTANALPGDRERCKAAGMDDYVTKPVKT
                     DDLGIILQRWAPVKAAAEVAPPAPARDLSKTDARVFDAGAMLANIGGDAELFDQLIRL
                     FLDRHSVMMKEIESAIGQADAAALERAAHSLKGTAGNLCAPDVVLLASQLEASGRLGT
                     LTEAPSLLVRLDRTVQELVAVLSRQINPTDSQ"
     gene            complement(13164..13643)
                     /locus_tag="PLS35_11340"
     CDS             complement(13164..13643)
                     /locus_tag="PLS35_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNI19306.1"
                     /translation="MKCQSTRFGTFEVNDDTLLVFPSGILGFPDWTKYVMLDHDTDAP
                     FKWLQCVEEPQLAFVILDPALFKPDYQVTITTDALIEIQKQESDEVSIVTILTIPSDD
                     PTAVTANLRGPLVMNHRTRLCKQLVLPEDLPTRYPLFAAASAAPSQSPSQPLQASAR"
     gene            complement(13737..13898)
                     /gene="csrA"
                     /locus_tag="PLS35_11345"
                     /pseudo
     CDS             complement(13737..13898)
                     /gene="csrA"
                     /locus_tag="PLS35_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
BASE COUNT         2944 a         4145 c         4122 g         2710 t
ORIGIN      
        1 gggtttgctg cgtcgatcga gcgcgatcaa gacacgctgt gtaaacgcct tggcgtcgca
       61 caggatgttg acatcgaacg gcacctgttg aaatttccgc tggaccatgg ccggatcgat
      121 ttcgaccaga tacttcttgg cctgtcgagc aaaactctga acgttgccgc cggtgatgcg
      181 tccagaaatg cgactgccga tggacaggag cagatcactg ttctgaatgc cgaagttgcg
      241 gccggcgcca ccataggtcc ctacccgtcc gccgtagttc tcataatcgg aactgatcac
      301 atccagcgcg ttccaggtcg gaaaacaggg tacgttcaac cggcgcccca aggcccgtac
      361 atcgtcctgt gcatcggcca ggcgcacgcc gcctcccacc agaatcaccg ggcgctccgc
      421 ctgctggagg tcgctgatga aacgggcgat ctgctgatcg acaaccgtga ggtcgaagct
      481 catggctgcg ggcggctcga agcccgtgag ctgtttggcg tcgaccatcg ctttttgcac
      541 gttgaggggt atatcgagca gcaccggtcc cggtcggccc tcttgacaca tccacagggc
      601 cttttcgagc tcgaaccgta cgtcttcagg attcaggatc atcttggcgt atttggtgac
      661 cggcgacgcc atcgcgacaa tgtccgtttc ctgaaacccg atttgccgga ttgagggatc
      721 ggggcgcaga aaccgcgagt tgatctgacc ggtgaggaag acacagggga tcgaatcgta
      781 gaagcaattg cccatcgagg tgagcagatt catcccgccc ggcccgctgg tcgcgatggc
      841 aaccccgggg atgcccttca ctttggcata ggcttcggcc gcgaacccgc cggcctgttc
      901 atgcatgacg gcgacatatt gggtcgcggc ggtccgtgtg aacgcatcga tcaaatcgcc
      961 gttggccgcg ccgtacacga cgaacatctt gtcgatgccc cgttccgcca acgtattgat
     1021 gatgaagtct gccaccttga tcatggatgc tcctttccct gcgctcatct acgccgcctg
     1081 atgcgacgcc tgatcatgat gacgacggcg cacctgatgc cgacacatct ccgctacgtg
     1141 agcgacgacg tgcgagccga acgttcgttg caacatggcc agatagctgg gatggccgaa
     1201 gtactcctga aaggcgcggt cgcgaaaatc caggacctgg gtggcggtca ggctgtcagt
     1261 cggcagcggg aggcattcgt acgcatgttg cgaatacccg atccagccgg ggccgccttg
     1321 atcatccggc aacgtccaat gatgctgttt ggcaagaccg tagagcgccg agccgggata
     1381 ggccatagca cagtaaaaat tcgcccattc ggtgttgagg gtcagcgcta aatccaacgt
     1441 cgcgcgcatg ctctcgaggg tgtcgtccgg aagcccgaaa atataatttg ccgcgacatg
     1501 gatgccgtga gcgcggatgc gctccaccgt ggcgacgatc tcacgttcac cgaaccggcc
     1561 cttctcgacc ccgtcacgga catgctggct gccggattcg atgccgagcc ccagccaggt
     1621 aaagccggcg cgcgccagct tctccagcac ttcgtcctgc accgtatcga cccgcgcgta
     1681 tgcccagatg ttcaggcgat agccccgttc gatgatgcgg tcgcagatac ccatgacatg
     1741 gcggcggttc aagacgaaca tttcatcggg aatcttgata ttcgagacgc cgtactcgcg
     1801 cacgagacgg tcgatctggc cgatgacatt gtcgggactc cagacacgca acatcggggt
     1861 ggtgaagggt gcgttgatgc agcagaagga acaggtaaac gggcatccca ggctcgtctg
     1921 aagcgaggcg taaggagttc tggcatccgg gttcccgaaa cagtgccaat tgtgggcgcg
     1981 atagcggctc atatcgagca gatcccacgc ttgccccggc aggtcgcggt cgagattcga
     2041 gaagagcggc gcgggcacat tgccgaccgc atgcccctcc tccaaatgcc acagtcccgg
     2101 gatctcgcgg agtgaatgct gcggggcgcg caaggcgacg acgaggccga gaattgtcgc
     2161 cggtccctcc ccttgacaga catacgtata gggttcctcc aacaaggtgc gtttgggcag
     2221 cgccgatgga tgggtgccca tcaccaatgt gggaatatcg gccagcgtat tcaggatctc
     2281 gcagactttc cggccggcgg gcatgcactg cgtggacgcc gacggttgtt gcccgtagat
     2341 gacgaagacc gccagcctgg gggcgatggc cgcgatctgc tcggctgtct gctgatgggt
     2401 cagcccttga gcctccgcat caagaatggc cacggagcaa ctgtgttgcc ggaggtaggt
     2461 ggcgatcaag cccgaccaga ccggcggctc gatggcggcg aagtcccggc tgagttcctg
     2521 gtagacctgg acgcggctgg gtggtgtcac cagcaagacg tcaagcggct tggacatggc
     2581 accctcccag tttcttggca aacgcgatgg tgcgcgacac cccctccagc acatcttctt
     2641 cctgtccgca caccgcaagt cggatacagg acgcgtaggc atcgccgaag cagcgacccg
     2701 gcgtcaccgc cgtatgttct tcctcaagca ggcgccggca aaacgtcata tcgtcgagcc
     2761 cggtcctgct gatgtcgaca aagagataaa acgccgcatc aggacggcgg cactgcaaca
     2821 caccgtcgtg agtgatgcgg tcatggcagg ttgcaatgag acgccgatta cgagcgcgca
     2881 cctcgacagc ataggcgtca agcacagaca atcctgcgag acagccgctc tgggtaaaac
     2941 cgggcaggca tgaatagagg gtggaattgg agagagctat tttggcgacc accgcctcgt
     3001 gcgccagcgc atacccggtg cgaaatccag gaacggacaa caccttggag aacgacgaca
     3061 tcgcgacgac atgcccggcc tgcggagccg cgagggtgac atgcgaacca gcgaaggtca
     3121 gatcggcata ggtttcatcg ctgaggagcc agattcctgc ctggccgcac cgtgccacca
     3181 gctcgtgcag gatcgccggc tcatagacgg cgccggtggg attattggca ctgttgacga
     3241 tgatcgccct aggctttgcc gccagagccg catcgatgga cggccgtgac agcgaaaagc
     3301 cgtcgcgtgc cgggagtggc acgggtacga catcaagtcc gagatgtgcg gctgcggccc
     3361 aataggaggg aaacgccggc gtgaagagca ctatccgctc gccgggattg caggtgatat
     3421 ccaggaactg gtgaatgagc atattcgccg ggctgatgac aacctgcgaa gcggcaatcg
     3481 gtttcccgct caacgaggcg tagcgttcaa caactgccga gcgcaattct tgtaagccgc
     3541 ccatcggcgc atatccgacg cgcttggcct gcagttcccc gatcgtggcc tcaagaattt
     3601 gagacggagg agccacgcgc ggattgccga gttcaaggtg ataaatgcgg tggccctggc
     3661 gttccagcgc ctgcgcctga tccaaaacac gaaacatttc ctgtccgatc aaacgttcgc
     3721 cgcgtgaaga gaaagccggt cgggtcttga actgcggcat cgacgctctc tgctgggtca
     3781 tcgtgcgatc gatccttgtg tctgatgaag aagcgaggtt gggaaatagg ccgaggtgtc
     3841 cttccagatt tcacggaaat gggccagacg ttcgggcgtc ccgatgtcgt gcaacggact
     3901 ggcggtgacg aatccgtaac actgatgcgt gacgagacgc ggcagcaggt cccgctccag
     3961 agaccagacc ggcatgtccg gaaagagtgt ggtcacggcg cgatcgaaca ggtagacgcc
     4021 ggcattatga tagttcgctg tccgcgttcg cggtttttcc accatcgaca agacgcggtc
     4081 accttctccc aacgccacgg cgccggtgtc ttcgcggctc tcatcgtggg tgacggcaat
     4141 cgtcgcgcca gcccgcttgc gcgtatggaa tcgcaacagc cgctcaggat cgatctcgca
     4201 gaacgagtcg ccgttcagca caagaaacgg gttgctgcgc accaatggca tggcatgcgc
     4261 cagtgcccca ccggtgccca aggggacagg atcacgcacg aacagcgttt cataggcccc
     4321 gcggtggcgc aggaaccagt cctcgatcat ctcgccgaaa tgtcccgtgc tgaaaataaa
     4381 gcggcgcgcg ccgtgacgca ggaaatgttc gaccagtaat gacacgaacg gtcgaccgtg
     4441 aatctccgcc atcggtttcg gacgatccga gacaacggat tgcagtcgcg ttccgaatcc
     4501 cccgcagaga atgatcacat cacaatcggc aaactgagcc atcaagccgc ctttccgatg
     4561 agtgtggcct tcagccgggc tgcggtctgc gcggagcttt gcgcccacac gtcatccagc
     4621 atcagctgat cttcctggta aaacacgatt tgagtgccca ggccctcaaa cttaaacggc
     4681 acctgcagca atccaggaag cgccagacga attcgctcat gatcttccgg cctggcgaag
     4741 agcagcatga atccgccccc gccggcaccc aggagttttc ctccgagcgc gcctgcttct
     4801 ctggccgccg cataaatcgc gtcgatagcc ggcgtcgtga tgcgcgaggt cagtcgccgt
     4861 ttgagcatcc aggcctcgtg cagcatgctg ccgaagtctg ccagatcgcg gtctccggta
     4921 agaatcgata caccttcatc gaccatctgc agcatcgtcg ataattcact ccggcgttgg
     4981 ttggtgcggt cgatctgctc gcgagccact tccgatgcaa tgcgtgaaaa gccggtgaag
     5041 tacaattgga gatggttttg aaacgccgca agccgctccg gacgcataat gatcggcgca
     5101 tggccgatgt caccgttctg aaaaaacgtc gccttgcaca agccgccatg tgccgcaagc
     5161 acctggtcct ggcatcccac ggcctcgccg agcacatcct gctcgacatg gatcgcagcc
     5221 tgagccagtt gcgctttgct gggcatgatg tgctgcaagg cgtacagcgt gtggagtaag
     5281 cccacggtaa aggaggaact ggatcccaag cccgttcgcg ccggcagatc gccgtcgtga
     5341 tgaatctcca gcccgtcgtc gatattgagg tacttcagca cgccgcgaac cgcgggatgc
     5401 tcgatctcct gattgttgtt caccagttcg atgcgcgaat acgcaattct ggtgcgatgc
     5461 tcgaagaacg gcggcaactg acggcagctg atatagcaat acttatcgat ggtcgttgcc
     5521 agcaccgctc cgccatgttc ccggaaccag acggggtaat cggtaccgcc accgaaaaac
     5581 gagatacgga acggcgttcg gctgattatc atcctcgccc tccctgccgg cttgcgctgc
     5641 gctcagacat tggcatattg accggctttg acgaccgtgt agcacttgat caactcacgg
     5701 atcccgcgct ccaaggacca ttccggcttg aacccggtct ccagcagtcg ctggttcgag
     5761 acgatgtaat cgcgcttatc cggatcctcc ccgatcgggg cttcgaaaga cacgaactgt
     5821 ggaaggactc gctggatttc tcggcagagt tccagtttcg agagattggc gtcgtccaac
     5881 ccgacgttgt acggcctgtt cttcatatcg tcgaagtggt cgatggcatg gagaaaggtg
     5941 cgcacgacat cccggatatg gatgtaattg cgtttgcagt ggccttcgaa caccaccacc
     6001 gctcgatcat gcaccgcccg ccagacgaaa tcgttgacca gcaggtccat gcgcatccgg
     6061 ggggacacgc cgaacaccgt cgccaggcgc agcgtaatcg cattgccacg gtccaggacg
     6121 acgcgttccg ccttcacctt cgtttcgcca tagagactga tgggacgaag cggcgagtct
     6181 tcggtgcagg gcacaccagg ttgcccgatg ccatacccgc tgttggtcac agggaaaatg
     6241 atctgctgcc gcggagagga cagtttgcag agcagttgca cggcttcgaa gttcaccgtg
     6301 taggccccga cacggtctct gtcgcacaag ggggccccga ccagggcggc aagcggaatg
     6361 atgatatcgg ctttgcgcag caggtctgtc accagccgct catcccggca atcgccgcgc
     6421 acgacttgaa atttctcctc ggcacagcaa tccatcaagc tgttttgccg gtacataaaa
     6481 ttgtcgagaa cggtgaccgc atacccccgg tcaagcaatc gtctggtcag cactgaaccc
     6541 agatatccgg cgcccccggt gatgaggaca tgtgtcgagc ctgtcgtcat agttacacgc
     6601 gctccttctg gctgtgccac tcgtgctgcc ctgctcccct gctcatgcag cctccaggac
     6661 gatacgggaa atgtgcagga catcctggtc ggtcattgag gcatggttcg gcagaaaaaa
     6721 tccgcaatga tggactcggt ctgccacagg gaaactggcc ttcccgtagc gattcatcca
     6781 gaacggatgc agacccagat tgccggctga gaagatgcgt gtctcgactc cctcggccac
     6841 caacgcccgg acgatgcgtt tccgttgttc cgggctctcg gccagtaagc cgaaagaaat
     6901 gcttgcgact ttgctgcctt tcggcggccg ctgcgtgtaa aaccgcgtcc ccagctgctc
     6961 gagatagcga tcgtgattgg cttgccgtcg gccggtcatc caatccagct tacgcaattg
     7021 ctcgaggccg atgaatgcgt tcagatcggt ggatcgcaga ttgaatcccg gttcgtagaa
     7081 cacgaagggc gaatgaaagt cgtccacctg atactggccc accaattcct ggtggcgcgc
     7141 ctggtctaca tctttgctcc acccgtggct gcgcaacatg aggagaagat tcgtgagctc
     7201 cttgtcgctg gatgagacca tcccgccttc gatcgtggac atttgatgcc cgaagtaaaa
     7261 cgaaaagctc gccatgtcgc cgaatgtccc gacctttttg ccgtcgtatt cggctccgat
     7321 ggcggcgcag gcatcttcaa ggagataaaa gccatatcga tctttcaacg tctgcagctc
     7381 acgcatccgg tggggtacac caaggacctg aaccagcagg accgtctgcg cgtgatgacg
     7441 cttcaggagg tcttcaagat gattgagatc gaggccgaat gtgtcgggat cggcctcgca
     7501 catgaacgga gtaaatccga attggatggc cggagcgatg gaggtgaccc atccgacgct
     7561 cgggacgatg acattggtat tgcgcaactt tcccgatcgc agcaaggcgt aatacatgag
     7621 gagattggcc gatgatcctg aattgcaatg caccgaatag gggcgtccca gccactgcga
     7681 ccagcgttgc tcgaaatcga cggtcaccgc gcccttggtg agtcgcggat aggtccgcag
     7741 ccagtcgatc aaatgatcaa tatcctgccg gtcgatcgta tcctgagcca ggcaccatcg
     7801 cgggttgagc ttggcactgt gtgcgtgaag cgtcatggta gtagtccctc tcgcgccaaa
     7861 aaggaggcct gcgtctgtca acagaccagg caccgcacca ggatactaat caagatgtat
     7921 gccactgagg atggcagagg gaggatggga aaccgtagga tttcttagtt ggagaactgc
     7981 tgcgggcttt gtaaccgggc aaaaaattcc cagtccccgg caaccgctgt agtctaggcg
     8041 gactcgaagg aatgtttctg aaggacgaac gattccagcg cttttctgac cgatcctccc
     8101 cacccccggt actcgatggt ttccttcccg tcgaaccgga attccaggcc gagatgcgtt
     8161 ccgtctgcct gcattgacac gtttcggacg acccctgtca gattgttgac gtggcccgtc
     8221 cccacgattt cgaattctgc gcgtatgacc atgccgggga aaaagtcctg catcggccgc
     8281 tgcaccagta ccgcgcatcc ggacgcgctg aggtctgtta acaatccgcc gatgcggcat
     8341 ggactaccgg ttggctgatt gcgcaggttc tgatccgctt gtaacagaac gatgggttcc
     8401 tgcgccgtca cgcgtccatg tttacggagc ggcacttcgt ccagcgtggc gggaaaagcc
     8461 agcagtaaaa atggaaacgg ctgcaactgc gcatggagga cgtccgtacg atatccgatc
     8521 atttttccat tatagatgta tcgcaacaga cagggagttc ccgcagcgca agaaatcggt
     8581 tggccgaaat gaaacggcca ttcacagacg atccagctgc gctctttcca tcccagcacc
     8641 gtggcgccgt actgggctcc ggtgccctcc gccggaaggc tgagttgcag cgagagcccc
     8701 acttcgagga atgaagctgg atgacgatgg acgatgagat cactcatgaa gtctccccgc
     8761 gcgagtcatg caaggcatag acacacccct gtcagcccta tcggcagatg ccttcagaat
     8821 ctgaagcctg tgtcacgctc gcgacatatt tctcagtaaa gaccgttctc acatcgaccg
     8881 atagatacaa gacagaaacc acaccctggg cgcactcagc acaggccttt tgaggattgg
     8941 gcataactct gacatggcat cgacaaaacg tatccctatc gatcagctca ccgtcggcat
     9001 gttcatcgca ggtttggacc aaccctggta caggacgccc tttctccttc acaagtggct
     9061 ggtatccaac cccgacgaca tcgtgcaact caaacggcac ggcattcaga ttgtcaccat
     9121 tgatacggat cgcgggttgg atgtaggcgc agctccagtg gccgcgccgg aatccactgc
     9181 tccggttcct gagccgtcgc ccgcttctgc agaatccgtc gatgcgtcga cgaacggcca
     9241 ttcttcgcat gccgcggcag cggccgcgac ataccgggaa gcgatggaag cggttgaacg
     9301 cgtgttcacc gaccttgaag ccggccagcc accgaagatt gcggcgctca aaccggtagt
     9361 gagcaaattg ttgaagcaga tcatcgaaca accggaagcg atgatgatcc agttttgtct
     9421 ggataagatg cgtcgcttcg acggcacgct ggccaatcat ggcatggatg tctgtgtgtt
     9481 gacactcatt ctggccgtgg aaaatagctg cacggaaagc gacatggagt cgctcggcct
     9541 cggcgcgctg ctgcacgata tcggcttcgt tcggctgccc cgcaacttgt accggaagac
     9601 ctcggccttg acggagcagg aacaagtcct gatgcggcaa cacccgcagt tggccgccac
     9661 ggtgttatcg aaaatcgact cgctccccga aacggtctcc caaatcatcg cgcaacacca
     9721 tgagtttcag gacggcagcg gattccccaa gttggtcaaa tcgggcggac tctcgccgct
     9781 ggcgcagttg atggccctgg cggataccta tgacgatctc gtgacctcgc gctatggccg
     9841 cgcaccgctg ttgcctcacg atgccatccg tcaactgttt gtgttgggcg cgaaaggccg
     9901 atacgataag aatctcgtcg aagtcgccat caagatgctg ggcgtctatc ccctcggaag
     9961 cctgatcaag ctcaatacga acgaatgcgc ggtcgtcatc ggcctcaatc atgaggaccg
    10021 tctgcgtccg cgtatccgca tcatcagagg gcccgatggg gaaattcaga atccccccat
    10081 cgacagtgat cttcaaaacc aggcggccga tcagccggtg cgcaccattc ttcgcgcgtt
    10141 ggatcctaag caggaacaga tcgacctccc gcaatatctt gatgtcgccg taggtggggc
    10201 ataagtatga gccgtccatc ccattcacgt tcacaccatc ctcaaccccc ttcggggttt
    10261 acgccgtcgc ctgaaatgat gggcctgctc tgggaagcgg tcccactggg gatttgtctg
    10321 ctggccccgg atcacaccat cgtcttcgcc aaccggatgg cggcccgtct gctgggccgc
    10381 tcgccggctg actgcgccgg gaaacgatgt tcggagctta ccggtcaacc ccttgaactg
    10441 aatgattcct tgcattcaac gggggtgtgg aaattccaga gcggcgccgc ggcggcatct
    10501 gatgagacca ccgatatgaa tgcgaccgat gcgccggccc tcaccgtgat cgaatgggag
    10561 caactgcgtc tttccggcat tccctctgtg tccacactgc tgacgctccg agacatttca
    10621 cgggaatgcg agttggagca ggatcgtgac cgtcttgcca ccgtggccga agaaagcccc
    10681 tgcccgattg tcgaaatcga ccggaatggc aatattctgt atgtgaaccc cgcgatggtg
    10741 gaggttctgt gccgattcgg atatgacccc aacggcaaac cggatatcct tcccgacaat
    10801 cttcccgcgc ttgtggccac ctgcctgctg gaagggcgat cgctgacctc acaggaagta
    10861 gtcagaggcg aggcgtgtta tagctggacg ttatgccccg tccccagcaa tcaactcgtt
    10921 cgtgcctacg gaatcgacct cagtgaggtg catgcgacgc atcgcgcgct gaacgccaca
    10981 gccgatcatc ttcgtgagag caatcgccaa ctggaccagg cgctgcagca ggcccaagcg
    11041 gccgcccgcg caaaatcgtc gtttttagcc atgatcacgc atgagttgcg cacgcccatg
    11101 aacggcgtga tcggtatggc cagcctgttg ctggacacgt ccctgaccga agagcaacgc
    11161 tcgtttaccc aaacgattca gcaatgcggc gaagcgcaac tgtcgctgat caatgacgtg
    11221 ctggaatgca gcaaaatcga agccggcaaa ttggaattgg agacactcga tttccaactc
    11281 cggaccaccg tggaagacgt gctgtcgcag tttgcggaac gggcgcagcg gaaaggcctc
    11341 gaaattaccg ggctggtcca tgccgccgtg cccaatgcgt tacgcggaga cccgggccgc
    11401 ctgcggcaag tcttgacgaa cttcgttgga aacgcggtca agtttactga acagggcgag
    11461 gtaacgctgc aggccttcct ggagagcgac acaccggccg gcgtgatgat caaatttgaa
    11521 gtcacggact ccggcatcgg gatcagcgaa gaagtgcaag ggcgtctgtt tcaggcattt
    11581 acccaggccg acagttccac gacacgaaag tacggcggca caggactggg actggccatc
    11641 tccaagcaac tggtggagtt gatgggcggg acagtggggc tccgcagcaa gcctggcgaa
    11701 ggaaccacct tctggtgcac ggccctcttc cagaaacaag cggtgtgttc ccctgccatc
    11761 gtcccttcgg cggaactgag cggtcgccgc attctcatcg tcgacgacaa cgagtcgaat
    11821 cgaacgattc tccatcatct ggtctcggga tgggggatgc acgatgggca ggcacgcaat
    11881 gccacggaag ccatgcacat gctggagcag gcagcggcga acggggaacc ctatgatgcg
    11941 gccgtgctgg atatgctgat gcccggcaaa gacgggcttc aactggccca ggacatccgc
    12001 gctcaccagc atggcgccgg agttcgcttg gttgttctga cgtcgctgat tcagcccggc
    12061 catgcggagc gggcgcggcg tgccggcttc acggcctatc tgaccaaacc ggtccgccat
    12121 gatcagctac aaggctgtct gcgggtggtc ttcgggatcc agcacaaggc agcgaccgca
    12181 tcagctgcga ccggcaaggg gcaacccatc tcgccgacgc tcatcacgcg tcacacattg
    12241 gccgaacaat ccccgcgtcc gcgaattctg gtggccgaag acaacgtggt caatcagaaa
    12301 ctggccgtgc gcatgctgga tcgccttggc tatcaacctg acgtggtctc caatggccag
    12361 gaagcggtca cggccttcga gcgggaatcc tatgcggcga tcgtgatgga ttgccagatg
    12421 ccgacgatgg acgggtacga agccacgagg ctcatccggg cgcaggaaga gcgaccggac
    12481 gcgtcccgga cgcgcgccca catccccatc attgcgttga cggcgaatgc gttgcctggc
    12541 gaccgggaac ggtgcaaggc cgccgggatg gacgactatg tcaccaagcc ggtgaagacg
    12601 gatgatctcg gcatcatcct gcaacgatgg gcgccggtga aggctgcggc agaggtcgcg
    12661 ccccccgccc ctgcccgcga cctgagcaag accgacgcgc gagtcttcga cgctggcgcc
    12721 atgctggcaa acatcggtgg cgatgccgaa ctgttcgatc aattgatccg gctgtttctg
    12781 gaccgccata gcgtcatgat gaaagaaatc gagtccgcta tcgggcaggc tgacgccgcg
    12841 gcgctggagc gggctgcgca cagtctcaag ggcacggcag gcaatctctg cgcccccgat
    12901 gtcgtgttgt tggcaagtca gctggaagca tccggacgac tcggaacgtt gacggaagcc
    12961 cccagcctgc tggtgaggct ggaccgcacc gtgcaggaat tggtggccgt gttatctcgc
    13021 cagatcaatc ccaccgactc gcagtgacac ttgcccatct gcgcgcgaaa cccaaccagg
    13081 cgaacgtcgc cgtctgaaac gctattggta gcacaaggta accaggcctg acgcggcttg
    13141 cgggtgacgt actgcaatgt ggcttagcga gctgaggctt gcaggggctg ggatggtgac
    13201 tgactgggcg ctgcggaagc ggcggcaaag agcggatagc gggtcggcaa atcttccggc
    13261 aacaccaact gcttacacag cctggtgcgg tggttcatga ccagcgggcc acgcagattg
    13321 gccgtcactg ccgtgggatc atccgaggga atcgtcagaa tcgtcacgat ggagacttcg
    13381 tcgctctcct gcttctgaat ttcgatcagg gcgtccgtcg tgatcgtgac ctggtaatcg
    13441 ggcttgaaga gcgcaggatc gagaatgacg aaggcgagct gtggttcctc gacacattgc
    13501 agccacttga acggcgcatc cgtatcatgg tcgagcatga cgtatttggt ccagtcggga
    13561 aagcccagaa tgccggatgg gaacaccaac aacgtgtcgt cgttgacttc gaaggtgccg
    13621 aatctggtcg actgacactt catggacggt tctcagctcc cgcggcgtcc tgttttattg
    13681 gacagttgac ggaaggcttc cagaggaatg actccggggc ctgcggcaat gcgattctcg
    13741 tcttgaatcc tcgcatgcac ttcatcgcga tgcacttgca cattgggcgg cgcttcgatt
    13801 cccagtctga cctgtccgcc tttgatgccg agcaccacaa tgcggacatc gggaccgatg
    13861 gtgacgccct ctccccgacg tcgtgttaag accagcatac aagccttcct tggcgtgtga
    13921 a
//
LOCUS       DAOQEW010000097        13889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1252_length_13889_cov_8.304395, whole genome shotgun sequence.
ACCESSION   DAOQEW010000097 DAOQEW010000000
VERSION     DAOQEW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_13889_cov_8.304395"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLS35_11350"
     CDS             <1..300
                     /locus_tag="PLS35_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNI19307.1"
                     /translation="IVADMTAKAFDFAEQVEGHGLLYFHDPMAVLAAIEPSLLKTEPL
                     HVEVEVVGRVSRGATVADRRHRRPEEKANPNMQVAVDVDAERALSVIRSRLCPWS"
     gene            291..1211
                     /gene="rbsK"
                     /locus_tag="PLS35_11355"
     CDS             291..1211
                     /gene="rbsK"
                     /locus_tag="PLS35_11355"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNI19308.1"
                     /translation="MVVVVGSSNIDFTAVVDRLPARGETLLGHRFLQSFGGKGANQAV
                     AAARAGADVAFLSKVGNDFNGDLIAQHLATQGLSRLMLLRDAAVQTGVAMILVDVAGD
                     NQIVVVPGSNQQLTPADVEQHAQLIADARVLLVQMEIPIDTVQQCLRLAKRYGLRTIL
                     NPAPACPLPPESLRMVDILTPNEREACVLSGATDPADAARVLVSTGAETVVVTRGTEG
                     AVVSRGKHVTHIPAFIVDAIDSTGAGDTFNGALACGLAEGLSIEEAVEMAAAAGALAT
                     TGYGAQAAMPARGDIDRLRRTGVRRMPPRG"
     gene            complement(1208..1849)
                     /locus_tag="PLS35_11360"
     CDS             complement(1208..1849)
                     /locus_tag="PLS35_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19309.1"
                     /translation="MNYPVIALWGLLGVALIGEQAVAGTAVQRLTAESMHECSLGRQA
                     QTRADRIQHFASGQAFGEQAVAADESSPDAHFALFCNLGEQLRVDGESLSSLFGFRRM
                     MKELNRTLELDPSHLDALSAKGTVLVRVPGFMGGDKEKGELLLRQVIAREPAAVNARL
                     SLAKSYCADGRHEEAVAIATKALDLAQELQRADFIPEARQVLAQLRAQSTKGN"
     gene            complement(1944..2486)
                     /locus_tag="PLS35_11365"
     CDS             complement(1944..2486)
                     /locus_tag="PLS35_11365"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HNI19310.1"
                     /translation="MPLSLPDHLAPNLNILFVGINPGLRSAAVGHHYAGYSNRFWKLL
                     YDAALVPEPLTYHDDERLLEWGLGLTNLVARPTAGSEHLTAEDYATGRRALMEKLRRY
                     RPRVTALVGITLYPILFPLEPKQAGRKPGLQSVTLYDSSIVLLPNPSGRNAAYPYHAL
                     LDAFRTLTPWGRTSLGSVLP"
     gene            2763..2987
                     /locus_tag="PLS35_11370"
     CDS             2763..2987
                     /locus_tag="PLS35_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19311.1"
                     /translation="MKKMMLTVMAAALLVAFSAPSFAGDDKKKEEKKGGHFSQTLFGD
                     DKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            complement(3064..3582)
                     /locus_tag="PLS35_11375"
     CDS             complement(3064..3582)
                     /locus_tag="PLS35_11375"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNI19312.1"
                     /translation="MVRPIYKGTVVTLNVDTVRLPNGHTIDLEVIRHPGASAVVPLKK
                     DGTVVLIRQFRHAANGFIYEIPAGKLHPNEDPLDCAARELEEEIGYKAGRFQLLSSIF
                     TAPGFADEVIHVYLATELTLGTQNLDQDEVLDVVEMPLKEAIDRIEDGTIRDAKTIVG
                     LQAVYIRQVGNR"
     gene            complement(3595..4704)
                     /gene="gcvT"
                     /locus_tag="PLS35_11380"
     CDS             complement(3595..4704)
                     /gene="gcvT"
                     /locus_tag="PLS35_11380"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNI19313.1"
                     /translation="MKQTPLIDAHRSAQGKLVDFAGWEMPIQYSGVVDEYQTVRRHAG
                     LFDVSHMGRIRVAGPGSLAFLQRVTTNDVSKLSVRQSQYSMVCAPNGGIKDDIFIYHV
                     KPYEFLVCVNASNREKIVAWFHEKVAQAQGCTVQDQSTTLAQIAIQGPASRDILTAVG
                     LTDLPLLKIRHCLDAVVCGHATLVTRTGYTGELGYELYLPAEGAMKIWNRLLEAGHPL
                     GMKPAGLGARDLLRLEMAYLLYGNDMDEGTSPIEAGADWVVKFDKGEFIGRTPLLAQH
                     NDGPSRRLVAFELVEKAVPRHGFKILSTQDQHAVIGEVTSGNLSPLLQKGIGMGYVTP
                     AASQPGSSILIDIRGKSCPAVVVKPPFYKKQPSTP"
     gene            complement(4761..5135)
                     /locus_tag="PLS35_11385"
     CDS             complement(4761..5135)
                     /locus_tag="PLS35_11385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI19314.1"
                     /translation="MMANSEQPGTTAEVTTASGLKYVDVVVGTGREAAAGNLATVHYT
                     GWLTNGKKFDSSVDRRDPFSFPIGAGQVIRGWDEGVAGMKVGGKRKLTIPPELGYGAR
                     GAGGVIPPNATLVFDVELLEVR"
     gene            complement(5259..6479)
                     /locus_tag="PLS35_11390"
     CDS             complement(5259..6479)
                     /locus_tag="PLS35_11390"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNI19315.1"
                     /translation="MTAPSTLRFASALTRHADAQAAAADLIHAIREQLGPSRIDVAFL
                     FISAQHADQAEALSEALRTALGPVTLVGCTGEGIIATGREIESGPAATLWAAHLPGVL
                     VQPLRLSFSSVHDQFSLRNWPDLDEVGDTAPVMMLFADPFSTPMQEVLSVIEEQYPGT
                     RTLGGLAGGGQDLGENRLFLDDQVLTDGLVGVALSGNIAVRSVISQGCRPIGDRFIVT
                     KSEHNVIQELGGMPALHCLQTVFEQLSSDERAQAQRALHIGIAMDEQRAQFTLGDFLI
                     RNLLGADQQTGAIVIGDVIQEGQTVQFQVRDAHSADEDLHALLAAPPQGVPSKPLGAL
                     LFSCCGRGKGLFGVPHHDASVLGEQLGAIPLAGFFAQGEVGPVGGRNFLHGYTASIAI
                     FSEPERGHQTGLNR"
     gene            complement(6531..6758)
                     /locus_tag="PLS35_11395"
     CDS             complement(6531..6758)
                     /locus_tag="PLS35_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19316.1"
                     /translation="MADYSMGGGLTLLTALEKSDKFADFLKTRMAQALETRDPTELHY
                     LLAQLDDYHSYMWRYYKKLATERPERMNPGV"
     gene            complement(6846..>7040)
                     /locus_tag="PLS35_11400"
     CDS             complement(6846..>7040)
                     /locus_tag="PLS35_11400"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNI19317.1"
                     /translation="FSLTHLADTHLNAVITATVEATEEAILNALTGATTVTGRDGFRA
                     EAISIPRLRALLSPDPVIGR"
     assembly_gap    7041..7140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7141..7874)
                     /locus_tag="PLS35_11405"
     CDS             complement(<7141..7874)
                     /locus_tag="PLS35_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNI19318.1"
                     /translation="MSHSHPGSFSIIACVVLVMTTGVHSGWSAETLSDVTEEPRVRAR
                     ALGLTLGQLEPGPLNAITDVPGVKVGQVTLRRGEGPLQPGQGPVRTGVTVIVPREDVW
                     HKKVPAGAFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVANGVISWMLRRYPG
                     IGITDDTLTPVVAECDDGRLNDIQGRHVSEADVMQALDDAASGPVSEGTVGAGTGMIS
                     YGFKGGIGTASRRLPAAAGGFTVGVLV"
     gene            complement(7923..9320)
                     /locus_tag="PLS35_11410"
     CDS             complement(7923..9320)
                     /locus_tag="PLS35_11410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI19319.1"
                     /translation="MKISFHGAARSVTGSRHVLEMPGSRILLDCGLFQGPRQEADRQN
                     RFLGFDPRSIQAVLLSHAHIDHSGALPVLSQHQFRGDVHMTRATADLTAVMLEDSARL
                     QENDCAYLNRKEHRRGQKCLKPFYETRDARAIIRRFVGARYGDTIKVTPRLTASFHDV
                     GHILGSAAIRLKYSARGNSTTVLFSGDLGRSHMPILRDPTPPPSCDVLIIESTYGDRL
                     HEEAGETLTKKAQQLVAHAKAHNSKIIVPAFALGRTQDLVMRIKHLVAEGQIDPLPIY
                     IDSPLASKVTDVFRKHPECYDEETYRTFTSEGDPFAARYIRYVSSPDESKRLNSVKGP
                     CVIIASSGMCEGGRVVHHLKHAIQDEANIIAIVGFQAEHTLGRRLVEGWDVVPLFGIP
                     TARRAQVVVFNGLSAHADRNDLLAYVRAISPPPQHIFVVHGEEKQALSLAAAIQAEYP
                     RTSIVVPHKESSYEV"
     gene            complement(9317..10174)
                     /locus_tag="PLS35_11415"
     CDS             complement(9317..10174)
                     /locus_tag="PLS35_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNI19320.1"
                     /translation="MGAGSSDESASRHPHSASSSYIPADRDIDFLQRDELRPLRLGLE
                     LLKPELIQIEQGIRSTIVVFGSSRLVEPSLARQALEAAKAELQIAPQDALRQQQVTMA
                     ERRLAMAHYYDIAREFGRLVSATCQINGQCDFVIVTGGGPGIMEAANRGAADAGAKSM
                     GLNITLPHEQQPNPYITPDLCFQFRYFALRKMHFLLRARALVVFPGGFGTLDELFETL
                     TLLQTGKTRNITIVLIGRAYWERLINWSMLADEGLIGPNDLALFHFAETAQQAWDLIS
                     QTHGGPAAR"
     gene            complement(10240..10614)
                     /locus_tag="PLS35_11420"
     CDS             complement(10240..10614)
                     /locus_tag="PLS35_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19321.1"
                     /translation="MMPLSGCNVARLTINTPLTPQTVSFITEGQTTLTDVVNKLGAPD
                     SITDAEGGAVVTYRFLNAKYSRVNFGWLVKPWSPVDPDLVFSRSGMGTDAFELLCDSN
                     WVVTHQAFVRHRPDPLFTPYPF"
     gene            complement(10667..11047)
                     /locus_tag="PLS35_11425"
     CDS             complement(10667..11047)
                     /locus_tag="PLS35_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19322.1"
                     /translation="MRRAGLLLGAMLLTLNACAFSRGTLGEEIKTDTVNSIKKGVTTR
                     ADVLAQLGAPDRLLQLNGRDLFQYYRYDAKVGSLLLILINFSRVSVKSDDLFVIINRD
                     GIVEDVIASKRTENLEFRFWPFGD"
     gene            complement(11085..11600)
                     /locus_tag="PLS35_11430"
     CDS             complement(11085..11600)
                     /locus_tag="PLS35_11430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI19323.1"
                     /translation="MISADQLRIRLATVQDVDVLTTFSAAMAWETEHRRLDLARLRLG
                     TQAVIDRPEQGQYYVADLHRNAPADTVTVGQLLLTYEWSDWRNAQFWWIQSVYVDPAW
                     RRQGVYRAMHQAVMALAQSRADVCGVRLYVEGDNTVAQGVYERVGLTPSTYRIYESDF
                     VLPKPSSPDRR"
     gene            complement(11597..12223)
                     /locus_tag="PLS35_11435"
     CDS             complement(11597..12223)
                     /locus_tag="PLS35_11435"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNI19324.1"
                     /translation="MTSSHRLGRIFFTGLLLLVPAWTTFLILAALFETLDSFLLNLIG
                     HQIQPYAPGLGILLLIGMVLMVGAIATQVIGQRFVRWAETTLEGIPLIRSIYLTLKSM
                     TDLLNYRARFGQSTVVAFPFPRDGLWALGFVMGPPPAALQVAPSVELVMVFVPTAIHP
                     FTGYLAMIPKTQLQPLNLLPEEALKLEFSAGLYRPLQGWLTAPAVKSS"
     gene            12366..12662
                     /locus_tag="PLS35_11440"
     CDS             12366..12662
                     /locus_tag="PLS35_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNI19325.1"
                     /translation="MRFIVSDAVQKKVNLDKDLLAILCCPETKQAVVLADDLLIQKVN
                     GAIERGVLKNKAQKAVTEKLDGGLVRSDNKILYPVREDIPVMLIDEGIPLDQVA"
     gene            complement(13173..13406)
                     /locus_tag="PLS35_11445"
     CDS             complement(13173..13406)
                     /locus_tag="PLS35_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19326.1"
                     /translation="MAREWIIFAICLGLGGHIALAVVLHAPDMWPWSKAWLYGLLSGL
                     TLYIVVQVLRSLWWVFRGRNGDEPDVPEDQHIR"
     gene            complement(13464..13832)
                     /locus_tag="PLS35_11450"
     CDS             complement(13464..13832)
                     /locus_tag="PLS35_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19327.1"
                     /translation="MNPTLQRAVTSFYNLVYEAQTFATTMSRIDTAEQEHYAGRIEGL
                     NWVLDRCQELEDMDANITPTTLQRVLTEVKSDLDHELSVQRREKGRRADGREEALTFV
                     ADYLSSLITATEIESAKTSV"
BASE COUNT         2879 a         3784 c         4271 g         2855 t
ORIGIN      
        1 atcgtggcag acatgaccgc caaggcattc gattttgcag aacaggttga agggcatgga
       61 ttgctgtact tccatgaccc catggcggtc cttgcggcca ttgaaccatc attgctcaaa
      121 accgagccgc tgcatgtcga agtggaagtg gtggggcgtg tgtcccgcgg agccactgtt
      181 gcggaccggc gccatcgcag gccggaagaa aaagcgaacc cgaacatgca ggtcgcggtg
      241 gacgtcgatg ccgagcgcgc attgagtgtg atccgcagtc gattgtgtcc atggtcgtag
      301 ttgtcggttc cagcaatatc gacttcaccg cggtagtcga tcggttaccg gctcgcgggg
      361 aaaccctcct cgggcatcga ttcctgcaat cgttcggcgg caagggggcg aatcaggctg
      421 tcgcggcagc gcgagccgga gccgacgtcg cgttcctcag caaagtggga aatgatttca
      481 acggcgacct gatagcacag catctggcta cgcagggctt gtcacgactc atgctgttgc
      541 gtgatgccgc agtgcagacc ggggtcgcga tgattcttgt tgacgtcgcg ggcgacaatc
      601 agattgtggt ggtgccagga agcaatcagc agctcacccc ggcggatgtc gagcaacacg
      661 cccagctgat cgccgacgcg cgagtcctgc tcgttcaaat ggaaattccc atcgacacgg
      721 ttcagcaatg tctacggctg gctaagcggt atggactccg tacgattctc aatccagcgc
      781 cggcctgtcc gttgccaccg gagtctttgc ggatggtgga tattctcacg cccaatgaac
      841 gtgaggcctg cgtactctcc ggtgccacag acccggccga cgcggccaga gtgttggtca
      901 gcaccggggc ggaaacagtc gtcgtcactc gcggtacgga gggagccgtc gtctcacgcg
      961 ggaagcacgt gacgcatatt ccggccttta tcgtcgacgc gattgattcg accggtgccg
     1021 gggatacctt taatggggcg ttagcctgtg gcttggctga aggcctgtcg attgaggaag
     1081 ccgtggaaat ggccgctgcg gcgggggccc tggctacgac gggatatggc gcgcaggcag
     1141 caatgccggc caggggcgat atcgaccggc ttcgccgaac cggagtcagg cgaatgccgc
     1201 cgcgtggtta attgcctttg gtggattggg cccgcagttg tgcaaggact tggcgagctt
     1261 ccggtatgaa atcggcgcgc tgcagttctt gggcaagatc cagggccttc gtcgcaatgg
     1321 cgacggcttc ttcatgtcgc ccgtcggcgc agtagctttt ggccagactc aatcgagcat
     1381 tgacggctgc tggttcacga gcaatcactt gacgcagcag tagttctcct ttttccttat
     1441 ctcctcccat gaatcccggc acgcggacca atacggtgcc tttggctgac agtgcatcca
     1501 aatgactcgg gtccagttcg agggtccggt tcaactcctt catcatgcgg cggaatccaa
     1561 acagcgacga gaggctttct ccgtccaccc gaagttgctc gccgagatta cagaacaggg
     1621 caaaatgcgc gtcaggtgat gattcatccg ccgcaacggc ttgctcgccg aatgcttgcc
     1681 ctgacgcaaa atgttgaatg cggtccgcac gagtctgcgc ctgccgtccc agggaacatt
     1741 catgcatgga ctcggcagtg aggcgttgca cggcggtacc ggcaacagct tgctcgccga
     1801 tgagagctac gccgagcaag ccccacaatg cgataacggg atagttcatg aagcaccgat
     1861 catgtgagat ggagagaaaa agaggacgaa tggtacagca acgacctcgc tgaatgccag
     1921 agtatagcat gtggtgggta gcgtcatggg agaactgatc ctagagaagt cctgccccac
     1981 ggtgtaagcg tgcggaacgc gtccaagaga gcgtgataag ggtatgccgc gttgcgtccg
     2041 ctcggattgg gcagcaacac gatactggaa tcgtacagcg tcaccgattg cagtccgggt
     2101 ttgcgccctg cttgtttggg ttccaaggga aagaggatgg gatagagcgt gatgccgacc
     2161 agcgcggtca cacgcgggcg atatcgacga agtttttcca tcagggctcg ccggcctgtc
     2221 gcatagtctt cggcggtgag atgttccgat ccggcagtcg ggcgcgcgac aaggttggtg
     2281 agtcccaggc cccattccag aagccgttcg tcgtcatggt aggtgagggg ttccggcacc
     2341 agggccgcat catagagcag tttccaaaag cggtttgaat agcccgcata gtgatggccg
     2401 actgcggccg agcgaagccc gggattaatg cctacgaata ggatgttgag attcggtgcg
     2461 agatggtccg gcaaggaaag tggcaacggt atccccgtgc atgatgtgtc tggaagcatc
     2521 tattggttga atgcgcctgg cctggtgtat cggatttgag agtcgcttaa tgaggcggtt
     2581 tagtatcggc aaggctattc tgccacagaa tggaccccgg cgttatgagg tatttgaacc
     2641 atttcaacag tcgatgtgca gaaaaacaat aagttacgcc cgccatccat acggcaagga
     2701 ccttgctata acgaacaccc gtcggcggtc ggttacttca cacctaacag gaggtagtca
     2761 gtatgaagaa gatgatgttg acggtcatgg cggcagcgtt gttggtggcg ttcagcgcgc
     2821 cttcgttcgc tggcgacgac aagaagaagg aagagaagaa gggcggccac ttctcccaga
     2881 ccctcttcgg tgacgacaag aagaaggaag agaagaaggg tggacacttg tcccagaccg
     2941 tgttcggcga agagaagaag aaagacgaga agaagggcgg ccactaagag tcacccgttt
     3001 tttggcgagg ctagctgtct gaagaggtct cccatcctga tggtgggagg cctcttcggt
     3061 cgtttaccgg ttccccacct gccgaatata gaccgcttgt aatccgacaa tcgtttttgc
     3121 gtcccgtatc gtcccgtctt ctattctgtc gatagcctct ttcaggggca tctctaccac
     3181 gtccagaact tcgtcctggt ccagattctg ggtgcccaag gtcagctctg ttgcgagata
     3241 gacgtggatg acttcatcgg caaagcccgg agctgtaaaa atgctggaca acagctggaa
     3301 gcgacccgcc ttgtacccga tctcctcctc aagttcgcga gcggcacagt ctaaggggtc
     3361 ttcgttcggg tgcagcttgc ccgccgggat ttcgtagata aacccgttgg ccgcatggcg
     3421 gaattggcgg atcagtacca ccgttccatc tttcttcagc gggacgacag ccgacgcgcc
     3481 gggatggcgg atgacttcga gatcgatcgt gtgcccgttc ggcaagcgca ccgtatccac
     3541 gttgagtgtg acgacggtgc ctttgtagat cggtctgacc atgacgtgcg cggactatgg
     3601 cgtgctgggc tgcttcttgt agaacggtgg tttgacgacg acggcagggc aggattttcc
     3661 tctgatatcg atcaggatgg acgaccccgg ttgggacgca gccggagtga catatcccat
     3721 gccgatgcct ttttgtagga ggggcgacag atttccgctg gtgacttcgc cgatcacggc
     3781 atgttgatcc tgcgtgctca aaatcttgaa cccatgccgt ggcaccgctt tttcgacgag
     3841 ctcaaaggcg acgaggcgcc gcgatggccc gtcgttgtgc tgtgccagca agggcgtccg
     3901 cccgataaat tctcccttat cgaacttcac gacccagtcc gccccggcct caatgggtga
     3961 ggtgccctca tccatgtcat tgccgtacag caggtaggcc atttcgagtc gcaacaaatc
     4021 gcgcgcgcca aggcccgctg gcttcatgcc aagtgggtgt cccgcttcaa ggagacgatt
     4081 ccagatcttc atggcgccct cggccggaag atacaactca tagcctaatt cgccggtata
     4141 gccggttcgt gttaccaggg ttgcatggcc acagacgacg gcgtccaggc agtgccggat
     4201 tttcaggagc ggaagatcag tcaggccgac ggcggtgaga atgtcgcggg aagcggggcc
     4261 ctgaatcgca atttgggcaa gggtggttga ctggtcctgg acggtgcatc cctgggcttg
     4321 cgcaaccttt tcgtgaaacc aggcaacgat cttttcacga ttggacgcat tcacgcagac
     4381 cagaaattca tacggtttga cgtgatagat gaagatgtcg tccttaatcc cgccgttcgg
     4441 cgcacagacc atcgagtatt gcgactggcg gaccgacagt ttggagacat cattcgtcgt
     4501 gacgcgttgc aaaaaggcca atgagccggg ccctgcgaca cggatacgcc ccatgtgact
     4561 gacatcgaac agtcccgcat ggcgccggac cgtctggtac tcatcgacca cgccggaata
     4621 ttgaatgggc atctcccatc cggcaaaatc gaccagcttg ccttgtgcgc tgcgatgcgc
     4681 gtctatgagt ggcgtctgtt tcatcggatg tgcgtgaaat ccagggtgaa gatacgcaga
     4741 ggccggacca tcatcgggcc ttatcggact tcgagcaatt ctacgtcaaa cactagcgtg
     4801 gcattgggag gaatcactcc gccggcgccg cgcgctccat acccgagttc cggtggaatt
     4861 gtcagcttcc gcttgccgcc gaccttcatg ccggccacgc cttcatccca tccgcgaatg
     4921 acctgccctg ctccgatcgg gaaggaaaac ggatcgcggc ggtcgacgga gctgtcgaac
     4981 ttcttgccgt ttgtcagcca gccggtatag tgcaccgtgg ccagattgcc tgcggcggct
     5041 tcgcgcccgg tgcccacaac gacgtctacg tatttgagcc cggatgcggt cgtgacttct
     5101 gccgtcgtcc cgggttgttc actgttcgcc atcattggcc tccctatggt catgaatacg
     5161 agccccacga taccgcaagt tgtccaccag aagcgagtcg tcatacgtac ctttcagttc
     5221 ctgtgtgacc tgaatcacaa ataggacact cggagcactc agcggttgag ccctgtctga
     5281 tgccctcgct caggttccga gaaaatggcg atgctggccg tatagccatg gagaaagttc
     5341 cgcccgccca cggggcccac ttctccctgc gcaaaaaatc cggcgagcgg aatagcgcct
     5401 aattgttcgc ccaataccga tgcgtcgtga tgcggaacgc caaagagtcc cttgccgcgt
     5461 ccgcaacaac tgaacagcag cgcgccgagc ggtttggacg ggacgccctg aggaggtgcg
     5521 gcgagcaagg catgaagatc ttcatccgcg gaatgggcat cccggacttg aaactggacg
     5581 gtctgtcctt cctggatgac atcaccgatg acgatcgcgc cggtctgctg atcggcaccg
     5641 agtagattgc ggatcaggaa atcgccgaga gtgaattgcg cgcgttgctc atccattgca
     5701 atgccgatat gcaatgcccg ttgtgcctgg gcccgttcat cgctgctgag ctgttcaaat
     5761 acggtctgca agcaatgcaa agccggcatg ccgccgagct cttgaatcac gttgtgctcg
     5821 gatttcgtga cgatgaagcg gtcaccgatg ggacggcagc cttgcgaaat aaccgagcgg
     5881 accgcaatgt tgccggacag ggccacgcca accaatccgt cggtcaacac ctgatcgtcg
     5941 aggaatagac gattttcgcc gaggtcctgc ccgccgccgg cgagcccgcc cagggtgcgg
     6001 gtaccggggt actgttcctc aatgacggaa aggacttctt gcatgggcgt cgaaaaggga
     6061 tcggcaaata gcatcatgac aggggccgtg tcccccacct catccagatc cggccaattg
     6121 cgcagcgaaa actgatcatg gacgcttgaa aaggaaagtc gtagcggttg gacgagcacg
     6181 ccgggcaggt gagcggccca gagagtggcc gccggtcctg attcgatctc acgcccggtc
     6241 gcgatgatgc cctcgcccgt acagccaacc agcgtgactg ggcccaaggc ggtgcgaaga
     6301 gcttccgaaa gtgcctcagc ctggtccgca tgctgagccg aaatgaacag gaaggccaca
     6361 tcgatgcgcg acggccccag ttgctcgcgg atggcgtgaa tcaagtcagc ggccgcagcc
     6421 tgagcgtccg cgtggcgtgt cagagcggag gcaaatcgta gcgttgacgg ggcagtcata
     6481 atcacgggat gtcctgctga atagggatgg acggcgtgcc gccgaggggg tcagacgcca
     6541 ggattcatgc gttcaggtcg ttcggtggcg agctttttgt agtaacgcca catgtacgaa
     6601 tgataatcat ccagctgcgc gagcaaataa tggagctcgg tcgggtctcg cgtttccagg
     6661 gcttgcgcca ttcgcgtctt taagaagtcc gcaaacttat cgctcttttc gagcgcggtg
     6721 agaagcgtga gtccgcctcc catggaataa tcagccatag ccctccgcat ccgatacaga
     6781 cgagccagga gaatagccgt gaggccctgt aaaagcaagg tgccggccgg cctcgttcgt
     6841 ctcgttcacc gcccgatgac tggatccggt gacaggagtg cacggagtcg aggaatcgag
     6901 atcgcctcgg cccggaatcc atcgcgtccc gtcacggtgg tggcgcccgt cagcgcgttg
     6961 aggatggctt cttccgtggc ctcaaccgtg gcggtaatca cggcattcaa atgcgtgtca
     7021 gccagatgcg tgagtgaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 accaacacgc cgaccgtaaa cccgccggcg gcagcaggaa ggcgccggga agctgtgccg
     7201 atgccacctt tgaatccata cgaaatcatg ccggtaccgg ctccgaccgt tccttcggag
     7261 accggcccgg atgccgcatc gtctaaggct tgcatgacat ccgcttcaga gacatggcgg
     7321 ccctggatgt cgttcaagcg accgtcatca cattcggcaa cgaccggggt gagggtgtcg
     7381 tcggtgatgc cgattcccgg gtagcggcgg agcatccagc tgatgacgcc attggcgacc
     7441 cgcggcacat tgagcgtatt ggtcagggcg atgggatatt cgagaaatcc cgattcggcc
     7501 acccacgcca gaccagtcat ttctccggtg ccattcagga cgaaggcccc ggctgggact
     7561 ttcttgtgcc agacatcctc tcgtggcacg atgaccgtga cgccggttct gactggccct
     7621 tgccctggtt gaagcggtcc ttctccacgc ctcaaggtca cctgcccgac cttcactccc
     7681 ggcacatcag taatggcatt caacgggccg ggctccaatt gtccgagtgt caggccgagg
     7741 gcgcgagcgc gtacccgcgg ttcttcagtc acgtcgctga gcgtctctgc gctccatccg
     7801 gaatggacgc ctgtcgtcat cacgagaacc acgcaggcga tgatcgaaaa ggacccgggg
     7861 tgtgaatggc tcatgttgac ggcatcctcc atacgtcatc ggctgtgaag cgcggagtga
     7921 cgctagactt cataactact ttctttgtgc ggcacgacaa tcgaagttcg tgggtattcg
     7981 gcctggatcg ccgccgccag tgacagtgcc tgtttttctt ctccatgcac cacgaagatg
     8041 tgttgaggcg gaggagaaat cgctctcaca tacgcgagta gatcgttccg atccgcatgg
     8101 gccgacaatc cgttgaacac gacgacctgc gcgcgccgtg ccgtgggaat gccgaacagc
     8161 ggtacgacat cccagccctc aaccagcctg cgtcccaggg tatgttctgc ctgaaagccc
     8221 acgatagcga tgatattggc ttcgtcctga atggcatgtt tgaggtgatg caccacgcgt
     8281 cctccctcac acatgccgga tgacgcaatg atgacgcagg gacctttcac gctgttgagt
     8341 cgcttacttt catccggtga tgagacatac cggatatacc gggcggcgaa gggatcgcct
     8401 tccgaagtga acgtgcgata cgtctcttcg tcgtaacact cagggtgctt gcggaacacg
     8461 tccgtgacct tcgacgccag cggtgaatcg atgtaaatgg gaagcggatc gatctggcct
     8521 tcggccacca aatgtttgat gcgcatcacg aggtcttgcg tccgtccgag tgcgaatgcg
     8581 gggacgatga tcttactgtt atgcgctttc gcgtgtgcga cgagctgttg cgctttcttg
     8641 gtcagggttt ccccggcctc ctcgtgaagt cgatcgccgt aggtggattc gatgatcagc
     8701 acatcacaac tcggcggcgg cgtcgggtcg cgaaggatgg gcatgtggga tcgtcccaaa
     8761 tcgccggaga acagcacggt ggtactgttg ccgcgcgcgg agtatttgag ccggatggcg
     8821 gcggacccga gaatgtgtcc gacatcatgg aaggaagcgg tgaggcgagg cgtcaccttg
     8881 atggtgtcac cgtaacgcgc gccaacgaaa cggcggatga tggctcgcgc gtcccgtgtc
     8941 tcatagaacg gcttcaaaca cttctgtccg cgacgatgtt ctttccggtt caggtaggcg
     9001 cagtcgtttt cttgcaggcg ggccgagtct tccagcatca cggcggtcag gtcggccgtg
     9061 gcacgagtca tgtggacatc cccgcgaaac tggtgttggc tcaggacggg aagggcgccg
     9121 gagtggtcga tatgcgcatg cgacaggagc acggcttgaa tcgagcgcgg atcgaatcct
     9181 aagaagcggt tttgccgatc agcctcctgc cgtgggccct gaaaaagacc gcagtccaac
     9241 aggattcggc tgcctggcat ttccagaaca tgacggcttc cggtgactga acgcgcggcg
     9301 ccatggaacg agatcttcat cgtgcggcag ggcctccatg ggtctgactg atcaggtccc
     9361 atgcctgttg agccgtttct gcaaaatgga agagcgcgag atcgttggga ccgatgagcc
     9421 cttcatctgc cagcatggac caattgatga gtcgctccca ataggcccgc ccgatgagga
     9481 cgatcgtaat gttgcgggtt ttgccggtct ggagaagggt cagggtctcg aagagttcgt
     9541 cgagcgtgcc gaacccgccg ggaaaaacga ccagcgctcg ggcacgaagg agaaaatgca
     9601 ttttacgtaa cgcgaagtag cgaaattgga aacacaaatc cggtgtgatg taggggttcg
     9661 gttgttgttc atgcgggagg gtgatgttga gtcccatgga cttggcgccg gcgtctgccg
     9721 ccccgcggtt ggcggcttcc atgatgccgg gtccgccgcc ggtgacaatg acgaagtcgc
     9781 actgcccgtt gatttggcac gtcgcggaca ccaggcggcc gaattcacgg gcgatgtcat
     9841 agtagtgcgc catggccagg cggcgttcgg ccatcgtcac ctgctgctgt cgcagcgcat
     9901 cctgcggcgc gatctgcaac tcggccttcg cggcttctag cgcctggcgc gcaagggaag
     9961 gttcaactag ccgggaactg ccgaagacca cgatcgtcga acggatgcct tgttctattt
    10021 ggatcaattc cggcttcagg agttccagcc cgagacggag cgggcgtaac tcatcgcgct
    10081 ggagaaagtc gatgtcgcga tcggcgggga tataggaaga agacgcgctg tgaggatgtc
    10141 ggctcgctga ctcgtcggag gaccctgcgc ccatgagcgg cattataacg agatgggagc
    10201 aaggcagcaa ttcgtgaacg gcgatggtgt ctgggcaggt taaaacggat agggtgtaaa
    10261 caatggatcc ggacgatgac ggacgaatgc ctggtgtgtc acgacccagt tagagtcgca
    10321 caggagctcg aatgcgtccg tgcccatgcc ggagcgagag aagacgagat ccgggtccac
    10381 gggggaccag ggcttgacca gccagccaaa gttcacccgc gagtacttgg cgttgagaaa
    10441 ccgataggtc accactgctc caccctcggc atcggtaatg gagtcgggcg cgcccaattt
    10501 gtttacgaca tcggtcagcg tcgtctgccc ctcggtaatg aaagacactg tttggggagt
    10561 cagtggagta ttgatggtca ggcgcgcgac gttgcagccg gacaagggca tcatcagtat
    10621 cgcggcgaaa aggaatgtgc gcgagacagt cctgggtaac acggcgtcag tctccgaagg
    10681 gccagaacct gaactccaga ttttcagtgc gcttcgacgc gatcacatct tccacgattc
    10741 catcgcgatt gatgatgaca aacaggtcgt cgcttttgac tgacacgcgg gaaaagttga
    10801 tcaagatgag gagcaagctg ccgaccttgg cgtcgtagcg gtagtattga aagagatcgc
    10861 gtccgttcag ttgcagcaga cggtctggcg cgccgagttg ggccaggaca tccgctcggg
    10921 tcgtgacgcc ctttttgatc gagttgacgg tgtccgtctt gatctcttcg cccagggtgc
    10981 ctcggctgaa tgcgcaggcg ttcagcgtta gcaacatcgc gcccagcaac aggcccgctc
    11041 gtcgcatatg tgatcctcct tatgcgtatc acccaccggg ccggctaccg gcggtcgggg
    11101 gaagaaggtt tgggaaggac gaaatcggat tcataaatcc ggtaggtcga cggggtaagt
    11161 ccgacccgtt catacactcc ctgggcgacg gtgttgtcgc cctctacgta cagacggacg
    11221 ccgcacacat ccgctcgcga ttgcgccagg gccatcactg cttgatgcat cgcacggtac
    11281 acgccttggc gtcgccatgc ggggtcaacg tagacgctct ggatccacca gaactgggca
    11341 ttccgccaat cgctccattc atacgtgagg agcagttgcc ctacggtcac tgtatcggcc
    11401 ggagcgtttc ggtgcaagtc cgcgacgtaa tattgtccct gttcaggtcg gtcgatgacg
    11461 gcttgtgtgc caagtctgag acgcgcgagg tcgagtcgtc ggtgttcggt ctcccaagcc
    11521 atggctgcgc tgaatgtcgt aaggacgtcc acatcctgca cggtcgccag tcggatgcgt
    11581 aactgatcag cggagatcat gacgacttca ccgccggggc tgtgagccag ccctggaggg
    11641 ggcgatacaa gcccgctgag aattccaact tgagcgcttc ttccggcaag aggttcagtg
    11701 gctgcaattg ggttttggga atcatggcga gatagccggt gaacgggtga atggcagtcg
    11761 ggacgaatac catgaccaat tcgaccgatg gagcgacttg tagtgcggct gggggtgggc
    11821 ccatgacgaa accgagcgcc cagagcccat cgcgcgggaa cgggaacgcg accaccgtac
    11881 tttgtccgaa gcgcgcgcgg tagttcagaa gatccgtcat gctcttcaaa gtcaaataga
    11941 tgctgcggat caatggaatg ccttcgagcg tcgtttcggc ccaacggacg aagcgttgcc
    12001 cgatgacctg tgtagcaatg gctccaacca tcagcaccat gccgatgagc agcagaatcc
    12061 ccaaccctgg cgcgtagggc tggatctgat gaccgatgag gttgagcagg aacgagtcga
    12121 gcgtttcaaa cagggccgcc aggatcaaga aggtcgtcca ggccggaacg agcaggagca
    12181 gtccagtaaa aaagatgcgt ccaagtcgat gagaagatgt cacgctgagt cctggtgttg
    12241 gtgcgcgaga ggggtaacga tcatctacga taccagatgg ggatgagttt tggtatctac
    12301 ttgatctgcc gatttttttg gactatgttt cgggtaacct ttgagtcacc ctgtcgggtg
    12361 agattgtgag attcatcgtg agcgatgccg tgcaaaagaa agtcaatctc gacaaagact
    12421 tgttggccat tctgtgttgc cctgaaacca agcaggcagt cgtattggct gacgatctgt
    12481 tgattcaaaa ggtgaacgga gcgatcgagc ggggagtatt aaagaacaag gcgcaaaagg
    12541 ctgtcacgga aaagctcgat ggcgggttag tgcgatccga taataagatt ctctaccctg
    12601 tgcgtgagga tatcccggtc atgttgatcg atgagggaat tccgctcgac caggtggcgt
    12661 agttttccca cctcgaatct tctcaggtta cctcacaagc atgttgatgt tggatgggtg
    12721 ccctcagggg gcaatgcacc ctgtctgtat cccttcgcgc ccttgttcgg gagttcctct
    12781 acatcgcgaa agacgaggac aggctggtgg agactacggg gctacgagac cgctctcgcc
    12841 tgaggcgctc tcggttttgg agcctgtaga ggttccgcat tgcgcgcaga gatactcgta
    12901 cagatttcca gtgggtaaca cgagaagcag gcgctggcgg gtgggagtcg cttgacggca
    12961 atgaccgcag tagagcaagg aggcgttaaa tgaatcgtat tgatccgttt gacggtggcc
    13021 ttgaggagac gcgggggtcc tccggggctg tgtggcacgc ggaggccaac caggtggtga
    13081 cggtctgttc gaacgtggct gcatggaggg tattctactg agagctggat agagcgcgca
    13141 atggcttata tgggatggtc gtcgagcaag ggtcagcgaa tatgctggtc ttctggaaca
    13201 tcgggctcat cgccgtttcg tccgcgaaaa acccaccaca gcgaccgcag gacctgcacc
    13261 acgatataga gagtcaggcc ggaaagtaat ccgtacagcc aggctttact ccacggccac
    13321 atgtccggtg catgcagaac gacggcgagg gcaatatggc ctcccaagcc gagacaaatg
    13381 gcgaagatga tccactcacg ggccatagtc gccctcggag gaggccataa cgaaggataa
    13441 gcctggagac aatcgacagg cggttacacg gaggtttttg cggactcgat ctcagttgcc
    13501 gtgatcaggc tggacaaata gtccgcaacg aaggtcaggg cctcctcgcg tccgtctgcc
    13561 cgtcgaccct tttcccgacg ttgcacggag agctcatggt ccaggtcgga tttcacttcc
    13621 gtcagtacgc gttgcaacgt cgtcggcgtg atgttggcgt ccatgtcctc gagttcctgg
    13681 cagcgatcca ggacccaatt gaggccttcg atccgcccgg cataatgttc ctgctcggcg
    13741 gtgtcgatgc gggacatggt cgtcgcaaag gtctgggctt cgtacaccag attatagaaa
    13801 ctcgtaactg cgcgttgtaa cgtgggattc atagtgctcc ttggctcatt aagaggttct
    13861 cgtgattata cctgagatta ggggggcga
//
LOCUS       DAOQEW010000098        13885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1253_length_13885_cov_7.161894, whole genome shotgun sequence.
ACCESSION   DAOQEW010000098 DAOQEW010000000
VERSION     DAOQEW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_13885_cov_7.161894"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..406
                     /locus_tag="PLS35_11455"
     CDS             128..406
                     /locus_tag="PLS35_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19328.1"
                     /translation="MKKQNARPAIMKPAIEPANPMMEMVWRLERLERQVQRLSELVRN
                     HAEDNDRLVRIVAEDRDVIRRELLRKHEHRVRVRKHLRDVSSKVDLEH"
     gene            530..1582
                     /locus_tag="PLS35_11460"
     CDS             530..1582
                     /locus_tag="PLS35_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19329.1"
                     /translation="MRHHGPVAAVDHVSFQVRRGEFFSILGPSGAGKTSVLRIVAGFE
                     DPDAGDVLIDGRSMLGVPPNRRPVNLVFQSYALFPHLTVFENVAFGLRMRRVPAALIA
                     EQVEQALAMVKLVGKERRKPAQLSGGEQQRVALARALVNKPAVVLLDEPLAALDQQLR
                     QAMQVELKSIQEQAGLTCVCVTHHQEEAMMMSDRIAVMHQGRMWQVGTPRDVYENPRN
                     LFVSRFLGVSNELPGRVDDVVEDGHLFHPADDQVPAFPVRAGSEASPGHSATWSIRPE
                     RLRMSRERPAGRDAVLNGFIEKVLFAGSETKYLVRVGRDRLWEARTTGAGESPHAPGD
                     PVFLHWALGDGRVFVE"
     gene            1585..2475
                     /locus_tag="PLS35_11465"
     CDS             1585..2475
                     /locus_tag="PLS35_11465"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI19330.1"
                     /translation="MPSDDVSHVSPASQDSCRSCWLAAPGLLWLALFFLVPLVLVFAI
                     SFASRGTYGGIVWEFTWANYVDLLHPLYGKILGQSLWYATLTTAICFLLGFPLAYVIA
                     RSPARWQPVLLLLVMLPFWTNFLVRTYAWMIMLRHDGLANNLLLGLGLITEPLELLYT
                     PTAVVIGLVYGYLPFMVLPLYVAVERLDPVLVEAAWDLYASRWAVVTRVVIPLTMPGI
                     VGGCILVFIPSIGSFITPDLLGGARSMMIGNLIQHEYLVVRDWPLGSAVSFVLMAIVM
                     AAVVLYYRQASPGDGPLEGR"
     gene            2472..3245
                     /locus_tag="PLS35_11470"
     CDS             2472..3245
                     /locus_tag="PLS35_11470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI19331.1"
                     /translation="MKQRSLGLNAVSLLAMLFLYGPIIVLVLYSFNAAHLSMTWRGGT
                     LKWYAALVHDEALLAATANSLLVALVSTIGATGLGGLLALGMEQMPHRRQQLLEGGLV
                     LPLVIPEVMMGVALMLLFVTVRVPLGLTTVILGHIVFNIPLVTIMIRTRLRKLDPMLR
                     EAAADLGADSWQVFRHVTLPLLRPAIWGAVLVAFTVSLDDFLVTFFTAGPGSTTLPLK
                     VYSMIKSGVTPEINALSALLLVVSMMGVAASLYLQRREV"
     gene            3246..4316
                     /locus_tag="PLS35_11475"
     CDS             3246..4316
                     /locus_tag="PLS35_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNI19332.1"
                     /translation="MRATIRERVLCCLCLVWLAGYLTGCGQRFDENRVETRPVLHYFT
                     WSDYVSPDIIQEFERREQVKVVIDTFSSNEELLAKLQSGATGYDVAVPSDFMVAIMIQ
                     QGLLSELDQAALSNTSQLEPHLQTLPFDPERRYSVPYLWGTVGIGYDSASIPTPPDSW
                     SILWDARYKGRISMLNDQREVFGAVLRSMGQSMNSTEPQVIESAKDRLLNQKPLVKTY
                     TSDHYDQLLASGEVVLAHGWGGQVARAMADRPSIRYVVPKEGATIWADCLVVLRTAPQ
                     KALAMRFINYLMEPTVAARTSERLRFASTSRSARELVDAATRDNPAVYPPLDQLARLE
                     WMRDVGKGIRLYDRAWTELKMQ"
     gene            4362..5759
                     /locus_tag="PLS35_11480"
     CDS             4362..5759
                     /locus_tag="PLS35_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNI19333.1"
                     /translation="MRALSSVDRYSRTMRDDMSQHKNGSPSGVRLWHQHPGLWVLIAL
                     LGLTLFGSGWVRAAESTTIPAGSFAPPVVRLNLSEAVALFLKQNFDLLIAKYGIESSK
                     GQQITARLFPNPVAQIGTVASFTHGNTAANSGALTMQVQQLFELAGKRGYRIESAGFG
                     VQSAEADFEDAIRQLGFTLKDAYYRVLVAQRRLALAEENRDRFARILDVNTIRFKKGY
                     IAEVDLIRIRLQVVDFQSQVIESIQEGESARADLRQLLRLSPASKLELTTEMDYRRVD
                     PDMGKLRSVALDVRPDIRSRRALVAQREADLKLAKAFRVPDLTIGAGYSIQGPRGPDN
                     PQMGILNLGVPLPLFNRNQGGIVQAEVGVQSAQAELDRTVNQVENQVDVAYHNLLQSR
                     RLVEAYLAGVLEDARSTFTIVERAYERGGATILDLLDAARTSRTIQQNFIEALFSYQR
                     NLFQLESSVGQEITS"
     gene            5756..6904
                     /locus_tag="PLS35_11485"
     CDS             5756..6904
                     /locus_tag="PLS35_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI19334.1"
                     /translation="MNSGAIPVIGLTVVLLFSACSQKQDSAPSPPSASSEPVVAPAVP
                     PSAGQIETAVVDFKPVQPELVLVGKIAYGEDRYSKISSPLQGRVVEVRAKLGDRVEAG
                     ATLLVIDSSDITAAYSEFVKEASELEYSTRAQELAKELYATKALALKDLKQAENDLIK
                     ARAEFRRAKERLLSLRIPAAELEKPLAQQRITSRFEMKSPLTGTVVERAVTPGQSVGG
                     DVGQVLFTVADLDRLQVVADVYERDLALVKVSQVGRINVEAYPGTDFASVVASIGDVV
                     DPNTRTIKVRAWVDNRDQRLKPEMFARLRLDIGDATPFLTIPKEAVVEIDGKHFVYVV
                     DGADHYSKHEVTVSNVSSGQVRILEGLTSGQRIVTKGAVLVKGQEVKG"
     gene            6954..10058
                     /locus_tag="PLS35_11490"
     CDS             6954..10058
                     /locus_tag="PLS35_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNI19335.1"
                     /translation="MMVRIVELSLVQRFLICALGFTLLFGGLYAFQQLDIVAYPDPSP
                     PMVEVITQFPGWSAEEIERQITIPIEVALNGMPGLTDIRSLSIFGLSDIKIYFDFNTQ
                     YFFDRQEVVNRLSTLSLPQNVQPALSPWWAIAEIYRYQLEGDGKASLTDLKTIQDWQL
                     RREFRRVPGIIDVTAFGGTTQEYHVDVDPGKLISYQVSLAQVMEALTNSNANVGGNYL
                     TVGAQNYNIRGLGLINGLADIENVMVAEKEGTPIFIKTLGSVSVGHRVRLGKVGIDDN
                     DDVVEGVILLQRGYKALSVLDRVREKVEELNKWKLPEGVKIKTFYDRTALIHTTVETV
                     TDILISGMVLVFIILFVFLGHLRASLIVALTIPMSLLFTFTMMVMIGQSANLISLGAI
                     DFGIIVDATLVMVESIFFHLGHDRRLGLTVPQQIVRAARQVGQPIFFSTAIIVVAFIP
                     LFTMTGVPGKIFAPMSITYGFALFGALLMAFTLAPVLCSLLLTGVVTEQDTRFVSAIR
                     RTYLAVLRWALAHNMKVIGAALLLLVAAFGALQFIGGEFMPALEEGNLWVRATMPVDI
                     SFDEAARLTGEIRQMFRKSPEVVTIVSQLGRPDDGTDPTSFFNAEFLANLKPHKDWRT
                     GLTKDQLVEEIEERLKVIPGIIFNFSQVIQDNVEEAMSGVKGENSIKLFGTDLKTMEA
                     KAGEIESVMRGVHGVKDLGIFRLVGQPNLLIQVDREASARYGLRVSDVNAVVQAAVGG
                     QAVTQVFEGERLFDLVVRFLPEFRRDEEAIGNILVNTPNGARIPLKQVATIKTQTGAF
                     IIYRENNERYIPIKFSVRERDLESTVKEAQAKMAQAVSLPERYRIEWAGQYDQLTAEQ
                     KRLTMIVPLSLVIILFLLYTTFNSLTNALLVLVTVPFALIGGIFSLVFTGTHFSISAA
                     VGVISTLGVAILGGVLLISRIEDFRRSGLELREAILKGADVQMRPILMATLAAAIGLL
                     PAALATGIGSQAQQPLARVVVGGMLTAAALILVVLPVLYEVVHRRTAPRTPLEAGEEP
                     PAPY"
     gene            10086..10889
                     /locus_tag="PLS35_11495"
     CDS             10086..10889
                     /locus_tag="PLS35_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19336.1"
                     /translation="MIGVGQQRMRQVVVLGLILLGLMGMRPEAGWAGSMVWPVQMPYE
                     APPKSEPVPDVSIAPNTKALTPEELQRAESLLPLLEGKQEFWAMGEFVHLGEPALPVL
                     TKALTMPGPRIRYNAIETMSMIKAPAATPALLEAAKLATEIPRIREHALRVAVRLDPQ
                     QAPPAIEAMAKDPNSVIRKAAAFEARYVRKKEVIQPLIDLLGDEEKFVAMSAVQSLWT
                     LTRHETEFHDWDASNKQDRQNWAQEWIEWWNQSKETFEIPDPKTRKQAG"
     gene            11000..11530
                     /gene="def"
                     /locus_tag="PLS35_11500"
     CDS             11000..11530
                     /gene="def"
                     /locus_tag="PLS35_11500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNI19337.1"
                     /translation="MAILAISKLGNPILRQQAQSVSPSEIKKAAFQQLIDDMFETMYD
                     EPGIGLAAPQVGRSQQLVVMDCPGEGGFPKTVLINPTIRFYGPEQVEGWEGCLSVDGL
                     RGKVTRPSTVRVTGLDRNGKPFDFEASGLYAVCIQHELDHLIGKLFIDRMTDFSTLTQ
                     MDEFQKYWQKEPASVI"
     gene            11600..13279
                     /locus_tag="PLS35_11505"
     CDS             11600..13279
                     /locus_tag="PLS35_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19338.1"
                     /translation="MLAVVTFVCAGLATALELVPPYLVKIVIDDVIQAKRPEMLMWVL
                     GALLGSYILRNLMSSMRVRFNNLLEQKAVHTLRMQVFGALQRLSLSYFENRSTGEIMT
                     RVTSDTEHVERIFVDGLEGLLTASLTLIGITIMMFFLNWKLALLSLVPIPILIVCAAW
                     FTKRVHKYYAEIRKSVADLNAYLQDALSGIKETIGFNQHEYERKRFETLSQTYSQNSL
                     RAMFLWSWYWPGMVFVGSTGTVLILWYGAEEVMAGNLTVGQLVMFLSYLGLFYTPINE
                     IHSLNHMLQHALAASERVFEILDTKAEVEDRPGAVRPVERLRGAVEYRQVVFGYRQEG
                     TVLSDVNLTVRPGERIALVGPSGAGKTSLLKLLMRFYDVRSGAVLVDGYDVRDLPVAF
                     LRGQIGLVQQEPFLFNGTVRQNIVYSDLSASHERVEAAARAARAHDFITRLPDGYDSW
                     IGERGVKLSVGQKQRVSIARVLLKDPPIIIFDEATSNIDTETEVKIREALNDLTKGRT
                     TFIIAHRLATLHDVDRIIVVERGTIVEEGHHDALMKRGGVYAGLYEAQFKI"
     gene            13462..13656
                     /locus_tag="PLS35_11510"
     CDS             13462..13656
                     /locus_tag="PLS35_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19339.1"
                     /translation="MVLIFESDPLDNEDARGRFYHVCRGRIDRTEITLPQGQSIYECA
                     VCGIDLEPEDFWIARQKGSV"
     gene            13698..>13885
                     /locus_tag="PLS35_11515"
     CDS             13698..>13885
                     /locus_tag="PLS35_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19340.1"
                     /translation="MAGSIGRKTVSVSRGLMMLCETCYDQVYAEKLPDARNFIADHEA
                     LFDTICMGCTDINRPLID"
BASE COUNT         2714 a         3807 c         4345 g         3019 t
ORIGIN      
        1 ccgattgtag caggacgaag ggggaataga aaggacgcag aatgattgac ttcaactccg
       61 actcggataa tactacgaat caacatgcgg ttccgacagc gggtcgggcc gaagagaagg
      121 agcggcgatg aagaagcaga atgctcgtcc ggcgatcatg aaaccagcga tcgagccggc
      181 caatccgatg atggaaatgg tgtggcggct ggagcgactg gagcggcaag tgcagcgcct
      241 gtccgagctc gtgcggaatc acgccgagga taacgaccgg ctcgtgcgca tcgtggcgga
      301 agatcgcgat gtgatccggc gtgaattgtt gcggaaacat gagcatcggg tgcgcgtgcg
      361 gaagcatctg cgcgacgtca gttccaaagt cgatctggag cattgatccg agaccgtcgg
      421 tgcggcggtc tgaccccagc cctcgatggc caccttttcc acttctcctg tcagtgcctc
      481 tggcccctct ctttcgtcga cagtcccgac gatcgacatt cgagccatcg tgcgacatca
      541 tggcccggtg gcggccgtgg atcacgtcag tttccaggtc cggcggggag aatttttttc
      601 cattctcggc ccgagcgggg ctggaaaaac gtcggtcttg cggatcgtgg ccggtttcga
      661 agatccggat gcaggcgacg tgttgatcga cggccgatcg atgctgggag tcccgccgaa
      721 ccgccgtccc gtcaacctgg tgtttcagtc ctacgcactc tttccccatc tgaccgtctt
      781 tgagaatgtc gccttcggcc tgcggatgcg ccgggttccg gctgcgctca tcgccgagca
      841 ggttgagcag gccttggcga tggtcaagtt ggtcggcaag gagaggcgga agcctgccca
      901 attgtccggc ggcgagcagc aacgtgtggc gttggctcgg gcgttggtca ataagccggc
      961 tgtggtcctg ctggatgaac cactcgcggc cctcgatcaa caactgcggc aggcgatgca
     1021 ggtggagttg aagtcgattc aggaacaggc agggctgaca tgcgtctgcg tgacgcacca
     1081 tcaggaggag gcgatgatga tgtccgaccg catcgccgtc atgcaccaag ggcgcatgtg
     1141 gcaggtgggc actccacgcg acgtgtatga gaacccgcgc aacctgtttg tgtctcgatt
     1201 tctcggggtg tccaatgaac tgccggggcg ggtggatgat gtcgtggaag atgggcatct
     1261 ctttcacccg gccgatgatc aggtgcccgc atttccggtg cgcgctggtt ccgaggcgtc
     1321 acccggtcat tcagccacct ggtcgattcg gccggaacgg ctcaggatgt cacgggaacg
     1381 acctgccggc cgagacgcgg tgctgaacgg cttcatcgag aaggtgctct ttgccggcag
     1441 cgagacaaag tatctcgttc gagtgggccg cgataggctg tgggaagcga ggacgaccgg
     1501 cgctggcgaa tcgccccatg ctccggggga tccggtcttt ctccactggg ccttgggtga
     1561 cgggcgagtg tttgttgagt gaccatgccg tctgacgatg tctcccacgt ctctcccgcc
     1621 tcgcaagact cctgccgctc ttgctggctc gccgctcccg gcttgctgtg gttagccctg
     1681 ttctttctcg ttccactggt gttggtgttt gccatcagct ttgcctcgcg cggaacctat
     1741 ggcggcatcg tctgggagtt tacgtgggcg aactatgttg atctgctgca tccgctctac
     1801 ggcaaaatcc tcggccagtc cttgtggtat gcgacactga cgacggcaat ctgttttctg
     1861 ctgggatttc ctttggccta tgtgattgcg cgcagtccgg cacgctggca accggtgttg
     1921 ctgcttctgg tcatgctgcc gttctggacc aactttctcg tgcgcaccta cgcgtggatg
     1981 atcatgttgc ggcatgacgg cctggccaac aatctgctgc tcggtctcgg actcattacg
     2041 gagccgctgg aattgctcta taccccgacg gcggtggtga ttggattggt atatgggtac
     2101 ctcccgttca tggtcttgcc gctgtacgtg gcggtggagc gactggaccc tgtcctggtc
     2161 gaagccgcgt gggatctcta tgcatcccgc tgggcggtgg tgacgcgtgt ggtgataccg
     2221 ctcacgatgc cgggcatcgt gggcggatgc atcctggtat tcattccgtc gatcggctcg
     2281 ttcatcacgc cggatctttt gggtggcgcg cgcagcatga tgatcgggaa cttgattcag
     2341 cacgaatatc tggtcgtgcg tgactggccg ctcggttcgg ccgtgtcttt cgttctgatg
     2401 gcgatcgtga tggcggcggt ggtcttgtac taccgtcagg ccagcccggg agacgggcca
     2461 ttggagggac ggtgaagcag cgatcgctcg gcctcaatgc cgtcagcctc ctggcgatgc
     2521 tgtttcttta cgggccgatt atcgtacttg tgctgtactc ctttaacgcc gcacacctct
     2581 caatgacctg gcgagggggg accctgaaat ggtatgcggc actggtccat gacgaagctt
     2641 tgctcgcagc gacggcgaac agtctgctcg tcgccctggt gtcgactatc ggcgccaccg
     2701 gtctcggagg gctgctggcg ctcggcatgg aacagatgcc gcatcgccgg caacagcttc
     2761 ttgaaggcgg tctcgtgctt ccgctggtca ttcccgaagt gatgatgggc gtggcgctga
     2821 tgctgttgtt tgtgacggtt cgcgtgccgc tcggcctcac caccgtcatc ctcgggcaca
     2881 ttgtgttcaa tatcccgctg gtcaccatca tgatccgcac gcgactgagg aaactcgatc
     2941 ccatgttgcg cgaagcggcg gcggatctcg gcgccgactc ctggcaggta ttccgccatg
     3001 tgaccctgcc cttattgcgg ccggcgatct ggggtgcggt gctggtcgcc ttcacggtct
     3061 cgctcgatga ttttctggtg acgttcttca ccgccggccc cggctcgacg accctgccgc
     3121 tgaaggtcta ttcgatgatc aagtccggcg tgactcccga aatcaatgcg ctatccgcct
     3181 tgctgttggt ggtctcgatg atgggtgtcg ccgcttccct gtacctgcag cgtcgcgagg
     3241 tctgagtgag ggcaacgatt cgcgagaggg tcctgtgctg tctgtgcctg gtgtggctcg
     3301 ccgggtatct cacggggtgc gggcagcgct tcgatgagaa ccgggtcgag actcgacctg
     3361 tgttgcacta ctttacctgg tcggactacg tgagcccgga catcattcag gaattcgaac
     3421 ggcgcgagca ggtcaaagtg gtgatcgata ctttcagcag caatgaagag ttgctggcga
     3481 aattgcagag cggcgccacg ggatacgacg tggcggtccc ttcggatttc atggtggcga
     3541 tcatgatcca gcagggcttg ctcagtgagc tggatcaagc cgcgttgtcc aatacatccc
     3601 agcttgagcc gcatctccaa accctgcctt tcgatcccga gcgccgctac tccgttccgt
     3661 atctctgggg caccgtgggg atcggatacg attccgcgag cataccgacg ccccccgaca
     3721 gttggtcgat cctctgggat gcgcggtaca agggccgcat cagcatgttg aacgaccagc
     3781 gcgaagtgtt cggggcagtg ttgcggtcga tggggcaatc gatgaacagc acggaaccgc
     3841 aagtgattga gtcggccaaa gaccgcttgc tcaatcaaaa gccgctggtg aagacgtata
     3901 cgagtgatca ctacgaccag ctgctcgcct ccggggaggt tgtgctggcg catgggtggg
     3961 gagggcaggt ggcccgtgcc atggccgatc gcccttccat ccgttacgtc gtgccaaagg
     4021 aaggcgccac catttgggct gattgtctgg tggtgcttcg gacggcgccg cagaaggcac
     4081 tcgccatgcg gttcatcaac tatttgatgg agcccactgt ggcggcacgt acgtcggagc
     4141 gactgcgttt tgcctccacc tcccggtcgg cccgggaact tgtggatgcc gcgacgcgag
     4201 acaatcctgc cgtctatcct cccctcgatc aattggcacg attggagtgg atgcgtgatg
     4261 tgggcaaggg aattcggctg tacgatcgtg cctggaccga actgaagatg caatgattgc
     4321 ggccatgctc attatttgaa atgctgcgaa gaatcagtat aatgcgtgcc ctgtcctcgg
     4381 ttgatcgata ttcccgcacc atgcgtgacg acatgtctca gcacaagaac ggctccccga
     4441 gtggtgtcag gttatggcat cagcatcccg ggctgtgggt cctgattgcg ctgctgggtc
     4501 tgaccctatt cgggagcgga tgggtccggg ccgccgaatc gaccacgatc ccggccggtt
     4561 catttgctcc accggtcgtc cgtctgaatc tgtccgaggc ggtggccctg ttcctcaaac
     4621 agaatttcga cctcctcatt gccaagtacg gcatcgagtc cagcaaggga caacagatta
     4681 cggcgcgcct ctttcccaat ccggtggctc agatcggcac cgtggcctcg tttactcatg
     4741 gcaataccgc ggcgaacagc ggcgccctca cgatgcaggt gcagcaattg tttgaattgg
     4801 ccggcaaacg cggctatcgc atcgaaagcg ccggtttcgg cgtgcaatcg gcggaagcgg
     4861 attttgaaga tgctatccgg cagctggggt ttaccctcaa agacgcctat taccgcgtgc
     4921 tggtggcgca gcgcagattg gccttggcgg aggaaaaccg ggatcgcttc gcccgcatcc
     4981 tggacgtcaa tacgattcga ttcaagaagg gctacattgc cgaggtcgac ctgatccgta
     5041 tccggctcca ggtcgtcgat ttccagtccc aggtgatcga atcgattcag gaaggcgaat
     5101 cggcacgggc cgatttgcgc cagttgcttc gtctctcacc ggcgtcgaag ctggaattga
     5161 ccaccgagat ggactaccgg cgggttgatc cggacatggg aaaactgcgc tcggtggcgt
     5221 tggatgtgcg ccccgatatc agaagcagac gggcgctcgt ggcgcagcgt gaagcggatc
     5281 tcaaactggc gaaggcattt cgggtgccgg atctgaccat tggcgccggg tattcgatac
     5341 aaggaccgcg cggaccggac aatccccaga tgggcatctt gaatctgggc gtgcccttgc
     5401 cgctcttcaa tcgcaatcag ggcggtatcg tgcaggccga agtcggtgtg cagtcggccc
     5461 aggcggaatt ggatcgcacc gtcaaccagg tggaaaacca ggtggatgtg gcctatcaca
     5521 atctcctgca gagccgtcgg ttggtcgagg cctacctggc tggggtcttg gaagatgccc
     5581 gttcgacgtt taccatcgtc gagcgcgcct acgaacgcgg aggcgccacg attctggatc
     5641 tcctcgacgc tgcgcgcacg tcccgtacga tccaacaaaa ctttatcgaa gccctgtttt
     5701 cgtaccagcg gaatctgttc caattggaaa gctctgtggg gcaggagatt acgtcatgaa
     5761 ttccggcgca atcccggtca taggcctgac cgttgtgctg ttgttctccg cttgcagtca
     5821 aaaacaagac tctgcgcctt ccccgccttc cgcctcttct gaaccagtcg tcgctccggc
     5881 cgttccgccg tccgccggcc aaatcgaaac cgcagtcgtc gacttcaaac cggtccagcc
     5941 ggaattggtg ctcgtcggga agatcgccta tggcgaagac cgatattcca aaatttcctc
     6001 ccccttgcag gggcgggtgg tggaagtgcg cgccaaattg ggtgatcggg tcgaggccgg
     6061 ggcgacattg ttggtcatcg acagctccga cattaccgcg gcctattccg aattcgtgaa
     6121 agaggcgtcc gagttggagt actcgacgcg ggcccaggaa ttggccaagg aactctatgc
     6181 cacgaaggcg ttggcgctga aagacctgaa gcaggccgaa aacgatttga tcaaggcgcg
     6241 ggcggaattc aggcgcgcca aggagcgact gttgtcgttg cgcattcctg ctgccgagct
     6301 ggaaaagccg ctggcgcagc agcgcattac ctcgcgcttt gagatgaaaa gtccgttgac
     6361 gggaacggtg gtggagcggg ccgtcactcc tggacagtcc gtcggaggtg atgtcggcca
     6421 ggttctgttt acggtcgccg atctggaccg attgcaggtc gtggcagatg tctacgagcg
     6481 ggacctggcg ttggtgaaag tcagtcaggt gggccgcatc aatgtggagg cctatccggg
     6541 aaccgatttc gcgtccgtgg tcgcgtccat cggtgacgtg gtcgatccca acactcgcac
     6601 catcaaagtg cgcgcgtggg tcgataaccg ggatcagcga ctgaagccgg aaatgttcgc
     6661 acggctccgg ttggatatcg gagatgccac cccgtttttg acgattccca aggaggcggt
     6721 tgtcgagatc gacgggaaac atttcgtcta tgtcgtggac ggggccgatc actactcgaa
     6781 gcatgaagtg accgtctcga atgtctccag cgggcaggta cgcatcctgg agggcctcac
     6841 atcgggccag cgcatcgtga ccaaaggcgc agtcctggta aaagggcagg aggtgaaagg
     6901 atgatcaggc cggtgatcgt gcgccactgc cgacaacagc gctctgaagc cacatgatgg
     6961 ttcgaattgt cgaactgtct ctggtccagc ggtttctgat ctgtgcgctc ggcttcactc
     7021 ttctctttgg cgggttgtac gcctttcaac aactcgatat cgtggcctac cctgatccct
     7081 cgcccccgat ggtggaggtg atcacccaat ttcccgggtg gtccgcggaa gagatcgagc
     7141 gccagattac gattcccatt gaggtcgcgc tcaatggcat gccggggctc accgatatcc
     7201 gctcactgtc gattttcgga ttgagcgata tcaaaatcta tttcgatttc aacacccagt
     7261 actttttcga ccgccaggaa gtcgtcaacc gcctctcgac gctcagcctt ccgcaaaatg
     7321 ttcaaccggc tctatcgccc tggtgggcca ttgccgaaat ctatcggtat caactggagg
     7381 gggacgggaa ggcgagcctg accgacctca agactatcca ggactggcaa ttgcggcgag
     7441 agttccggcg cgtacccggg atcatcgatg tgacggcctt cggcggaacg acgcaggagt
     7501 atcacgttga tgtcgatccg ggaaaactca tcagctacca ggtcagcctt gcgcaggtca
     7561 tggaggcctt gaccaacagc aatgccaatg tcggcgggaa ttacctgacc gtgggcgcac
     7621 aaaattacaa catccgcgga ctcgggctga tcaacgggct ggcggacatc gaaaacgtga
     7681 tggtcgccga aaaagaaggg accccgattt tcatcaagac cctggggagc gtgtcggtcg
     7741 gccatcgcgt gcgcctgggc aaggtcggca tcgatgacaa tgacgacgtg gtggaagggg
     7801 tcattctgct ccagcgaggg tataaggcac tctcggtcct ggatcgcgtt cgggaaaagg
     7861 tcgaggagtt gaataagtgg aagctgccgg aaggcgtcaa gatcaagacg ttctatgatc
     7921 gcacggcact gatccatacg acggtcgaga ccgtcacgga tattctgatc agcggcatgg
     7981 tcctcgtgtt tatcatcctc ttcgtgttcc tggggcattt gcgcgcctct ctcattgtcg
     8041 cgctgaccat tccgatgtcc ctgctgttca cctttacgat gatggtgatg atcgggcagt
     8101 ccgccaactt gatttccctg ggcgccatcg atttcgggat catcgtggat gcgaccctgg
     8161 tcatggtgga aagcatcttt ttccatctgg gacatgatcg ccgcctgggt ctgacggtgc
     8221 cacagcagat cgtgcgtgcc gcccgccagg tggggcagcc gattttcttt tctaccgcca
     8281 tcatcgtggt ggccttcatt cccctgttca cgatgaccgg cgtgccggga aaaatctttg
     8341 cgccgatgtc gatcacctat ggattcgctt tgttcggcgc cttactcatg gcctttacgc
     8401 tagcccctgt cctatgctcg ttgctcctga ccggtgtggt gacggaacag gacacgcgtt
     8461 tcgtcagcgc gattcgccgg acgtacctgg ccgtcctgcg atgggcgctg gctcacaata
     8521 tgaaggtcat cggtgctgcg ttgctgctgt tggtggcggc gttcggtgcc ctgcagttca
     8581 tcggcgggga attcatgccc gcattggagg aagggaacct ctgggtgcgg gccaccatgc
     8641 cggtggatat ttcgttcgac gaagcggctc gattgaccgg agaaatccgg cagatgttca
     8701 ggaaatcgcc ggaagtcgtc acgatcgtct cgcaactcgg cagacccgac gacggcaccg
     8761 atcccaccag ttttttcaat gcggaatttc tggccaatct caaaccacat aaggactggc
     8821 ggacgggact caccaaagat cagctggtgg aggaaatcga agaacgcctc aaggtcattc
     8881 ccggcatcat ttttaatttc tcccaggtga ttcaggacaa tgtggaagaa gcgatgtccg
     8941 gcgtaaaagg cgaaaattcc atcaagctgt tcggcaccga tctgaaaacg atggaagcga
     9001 aggcgggaga aatcgagtcg gtgatgagag gcgttcacgg ggtcaaagat ttagggattt
     9061 tccgcctggt gggccaaccc aatcttttga tccaagttga tcgggaagcc agcgcgcggt
     9121 atggcctgcg cgtgtccgac gtcaacgccg tcgtgcaggc ggcggtgggg gggcaggccg
     9181 tgacgcaagt gttcgaaggg gagcggttgt tcgatttagt tgtccgcttt ctccccgaat
     9241 ttcggcggga tgaggaggcc atcggtaata ttctggtcaa tacgccgaat ggcgcgcgca
     9301 ttcccctgaa acaggtggcg acgatcaaga ctcagaccgg agccttcatc atctatcggg
     9361 agaacaatga acggtatatt ccgatcaagt tcagcgtgcg cgagcgcgac ctggaaagca
     9421 ccgtgaaaga ggcgcaagcg aaaatggctc aggctgtctc gcttcccgag cgctaccgga
     9481 tcgaatgggc gggccaatac gatcaactca cggcagagca aaagcgcctc acgatgattg
     9541 tgccgttgag cctggtgatc atcctctttc tgctctatac cacattcaat tcgctgacca
     9601 acgccttgct cgtcctggtg accgttccct tcgcgctgat cggagggatt ttttcactgg
     9661 tgttcacggg aacgcacttc agcatctctg cggccgtggg agtcatctcg acgctgggag
     9721 tagcgattct cgggggagtc ctgttaatct cccgtattga ggattttcgg cgcagcggcc
     9781 tggaattgcg tgaggccatt ttgaagggcg cggatgttca gatgcgtccg atcctgatgg
     9841 ccacgctggc ggcagccatc ggcttgcttc cggccgcact ggcgacgggg atcggctcgc
     9901 aggcacagca acccctcgcg cgggtcgtgg tgggaggaat gttgactgcg gcggcgctga
     9961 ttctcgtggt gttgccggtc ttgtatgaag tggttcaccg gcgcacggcg ccgcgaacac
    10021 cgttggaagc cggagaggag ccaccggccc cttattaacg acaacgtgtg cgagaggatg
    10081 tgatcatgat cggagtcggg cagcagagga tgcgtcaagt ggtggtgctg ggtctgattc
    10141 ttcttggcct gatgggaatg cgcccagagg cgggctgggc tggcagcatg gtctggccgg
    10201 tgcaaatgcc gtatgaggcg ccgcccaagt cggaaccggt gcccgatgtg tcgatcgccc
    10261 ccaatacgaa ggccctgacg ccggaggaac tccagcgggc ggagtcgctc ttgccgctct
    10321 tggaaggaaa acaggaattt tgggccatgg gagaatttgt ccatttgggc gagcccgcgt
    10381 taccggtgct caccaaggcg ctcacaatgc ccggccctcg tatccggtac aatgccattg
    10441 agacgatgtc catgatcaag gccccggctg ccacgcccgc cttactcgaa gcggcaaaat
    10501 tggcgacgga aattccccgt atccgtgaac atgcgttgcg ggtggccgtg cggctcgatc
    10561 ctcagcaggc acctcccgcc atcgaggcta tggccaagga tcccaattcg gtgatccgca
    10621 aggcggcggc gttcgaggcc cggtatgtgc ggaaaaaaga agtgattcaa ccgttgatcg
    10681 atctgctggg ggatgaagaa aagtttgtcg cgatgtccgc cgtccaatcg ttatggacgt
    10741 tgacccggca tgagacagag tttcacgatt gggatgcctc caataaacag gatcggcaga
    10801 actgggcgca ggagtggatc gaatggtgga atcagtcaaa ggaaaccttt gaaattcctg
    10861 atccgaagac ccgcaagcag gccggctgac agaggaacgc acgaggcggt tgcacagcct
    10921 caaaagcccg tggtaggtac atacgcaggc aatagcggcg tgaccaaagt ggtcgcgcgt
    10981 aggcagataa aggtggatca tggccatatt ggcaatcagt aaactgggga atccgattct
    11041 tcgacagcag gcacagtcgg tctccccgag cgagataaaa aaggcagcct ttcagcagct
    11101 catcgacgac atgtttgaaa ccatgtatga cgaacccggc atcggcctgg ccgccccgca
    11161 ggtcgggcgt tcgcagcaac tcgtggtcat ggattgtccc ggggaagggg gcttccccaa
    11221 gacggtgctc atcaacccga cgattcggtt ctacggccct gagcaggtgg agggctggga
    11281 aggatgtctg agcgtggacg ggttgcgggg caaggtgacc cggccgtcaa ccgtgcgtgt
    11341 gaccggattg gatcgcaacg gcaaaccgtt cgattttgaa gccagcggac tgtatgccgt
    11401 gtgtatccag catgagctcg atcatctcat cggcaagtta tttatcgacc gcatgaccga
    11461 tttctccacg ctgacgcaga tggatgagtt tcagaagtat tggcaaaagg aaccggccag
    11521 tgtcatctga tcccttccgg gtcgccgggt cgttgtgaac tcgctcctac gcgttctgtc
    11581 ctacctcaag ccgttccgaa tgctggcggt ggtgaccttt gtgtgcgcgg ggctcgccac
    11641 ggcgttggaa ctggtaccgc cgtatctcgt caaaatcgtc atcgacgatg tcatccaagc
    11701 caagcggcct gagatgctca tgtgggtatt aggcgccctg ttggggtcgt atatcctgcg
    11761 gaacctcatg agctcgatgc gggtacggtt caacaattta ctggagcaga aagcggtcca
    11821 tacgctgcgg atgcaggtat tcggggcctt gcaacggtta tccctgagtt acttcgaaaa
    11881 ccgctccacc ggcgagatca tgacccgtgt gaccagcgat accgaacatg tcgaacgcat
    11941 cttcgtcgac gggcttgaag ggttgctgac tgcctccctc accctgatcg gcattacgat
    12001 catgatgttt tttttgaatt ggaaattggc actgctgtcg ctcgtgccca ttccgattct
    12061 gattgtgtgt gccgcctggt tcacgaagcg ggtgcacaaa tactacgccg agatccgaaa
    12121 gagtgtggcg gacctcaatg cctacctgca agacgccctg tcgggcatca aggaaaccat
    12181 cggcttcaat caacatgagt acgaacggaa gcgttttgaa accctgagcc agacctacag
    12241 ccagaatagt ttgcgggcca tgtttctgtg gtcgtggtat tggccgggca tggtgttcgt
    12301 cggcagtacc ggcacagtcc tcattctctg gtatggagca gaagaagtga tggccggcaa
    12361 tttgacggtc ggccaactgg tcatgttcct gtcctatctc ggcttgtttt atacgccgat
    12421 caacgagatt cattcgctga accacatgct gcaacatgcc ctggcggcca gcgagcgcgt
    12481 gttcgaaatt ctcgacacca aagcggaggt cgaggatcgg cccggggccg tacggcctgt
    12541 cgaacggttg agaggcgccg tggaatatcg gcaggtagta ttcggctacc ggcaagaagg
    12601 cacggtctta tccgatgtga atctcaccgt cagacccggg gaacgaattg ccctggtagg
    12661 tcccagcggc gcaggcaaaa catccttgct gaagctgctg atgcggtttt acgatgtccg
    12721 cagcggggcg gtgttggtgg atggttatga tgtccgggac ctgccggtcg cgtttttgcg
    12781 ggggcagatc ggcctggtgc agcaagagcc ctttctcttt aacgggacgg tgcgacagaa
    12841 tattgtgtac agcgacctct cggccagcca tgagcgcgtc gaagcggcgg cccgcgcggc
    12901 acgggcccac gactttatca cccggctgcc agatgggtat gatagctgga tcggcgagcg
    12961 gggcgtcaaa ttgtcggtcg gacagaagca gcgggtctcc attgcccgtg tcctgctgaa
    13021 ggatccgccg atcattatct tcgatgaagc tacgtccaat atcgataccg agaccgaggt
    13081 caaaatccgt gaagcgctga acgatctgac aaagggacgt accaccttca ttattgcgca
    13141 ccgcctcgcg acgttgcatg acgtagaccg aattatcgtt gtggagcggg gcacgatcgt
    13201 ggaggagggc caccacgatg cgctcatgaa gcggggcggg gtgtatgcgg gactctatga
    13261 agcgcagttc aagatttagc aggctgttga gaaaacccgc cagcggcgtt ctcggccgca
    13321 cgtctccttg cgacgtaccc agagggtacg actcagtcgc cgggcggcct gcggcctggc
    13381 tggacggcct ttctgaacag cctgcgacgg cggagaagaa cgacgtattc tgaccagtta
    13441 cgaaggagcg acaggaaaaa catggtgctg atattcgaaa gtgatccgct cgataatgag
    13501 gatgcccggg ggcggtttta tcatgtgtgc cggggtcgaa ttgatcggac ggaaattacc
    13561 ttgcctcaag ggcaatcgat atatgagtgt gcggtatgcg ggattgatct ggaaccggaa
    13621 gatttctgga tcgcgaggca aaagggatcg gtgtgacggt tggtcgcacc gagtgacgat
    13681 agacagagga gtgcgttatg gctggaagta taggccggaa gactgtatcg gtttcacgcg
    13741 ggctgatgat gttgtgcgag acctgctacg atcaggtgta tgccgagaag ctgcctgatg
    13801 ccaggaactt tatcgcggat catgaggcgt tgttcgatac gatttgtatg ggttgcaccg
    13861 acatcaatcg tccgctcatc gacga
//
LOCUS       DAOQEW010000099        13867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1257_length_13867_cov_7.185491, whole genome shotgun sequence.
ACCESSION   DAOQEW010000099 DAOQEW010000000
VERSION     DAOQEW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_13867_cov_7.185491"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLS35_11520"
     CDS             <1..748
                     /locus_tag="PLS35_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNI19341.1"
                     /translation="ARKGLRRPAKDQYHDALPRTPQPRMVQASLKRYFLTGLLVMIPI
                     WGTILILKTLFVSLDGILGDAAARLVTPGYYVPGLGIIALILLIFMTGLFAANFIGRQ
                     VVRQWESLLNRVPVVRGIYSTIKSMMDILSFAERESYRRVVLIQFPKNGHYCFAFVTG
                     VTKGEMQQLSPDPLVHVYVPTSPNPTSGYFLLVPEREVIAVDITVEEAMKLIVSGGLY
                     TPTQPSGAGSQAGGKTWTPIKQPDAGVQVG"
     gene            800..2413
                     /gene="glmU"
                     /locus_tag="PLS35_11525"
     CDS             800..2413
                     /gene="glmU"
                     /locus_tag="PLS35_11525"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNI19342.1"
                     /translation="MTHASHDNASSSSIPGLGVIIMAAGLGKRMKSALAKVLHPVAGR
                     PMVLYVLDIACGLAEQGVAVVVGHQGADVRKVVEAVGGQVAVAEQTKQLGTGHAVLQA
                     RPVFGDAAHRKPTRYLILNGDTPLLTEATVRELLAMHDAQGAAVTLLTAVLDDASGYG
                     RVIRHRREEWLQGAADNRVQSIVEDKDASPAERSVREINVGTYVVDGEFLFPALDKLD
                     PRNAQGEYYLTDIVQMAVQQGRTVSALRLRDIDEGLGINSRVQLAEAEGVIRRRIRER
                     WLEAGVTMLDPASAWIDADVTIGRDTLLYPNVTLEGRTVIGENTVVHSGTRITDCTIG
                     QRVEILDHCIFRESQVEDDSHLGPFVHLRPGVVVRRKGKVGNFVEMKKTELGEGSKAN
                     HLSYLGDAMIGQGVNIGAGTITCNYDGYKKFHTVVGDGVFIGSDVQLVAPVTVGQGSV
                     IAAGATVTQDVPADALVIARVPQVTREGWAARRRALQSGQVPPPAPKPVPPVKARASA
                     TPAKAASKPKPRTRAAKKKQPAVQRSKRR"
     gene            2423..4255
                     /gene="glmS"
                     /locus_tag="PLS35_11530"
     CDS             2423..4255
                     /gene="glmS"
                     /locus_tag="PLS35_11530"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNI19343.1"
                     /translation="MCGIVGYVGNQDAVPILLNGLSKLEYRGYDSAGVAIQRGEKIDI
                     RRSVGKLINLQNSLAQKAIAGMCGIGHTRWATHGKPSEQNAHPHRSENCVLVHNGIIE
                     NYVELKQRLIKDGYKFQSETDTEVVAHLIDTHIKKDKLHLADAVRATAREIRGSYAIA
                     VISEHEPGLLVAARSGCPLVIGRTAEASFVGSDVMAMLSHTRDVTFLEEGDVAEVTAG
                     GVTFTDLDGRAVTRKKTKVTWDASAAEKSGYPHFMLKEIHEQPQTILDTIRGRYSYES
                     GEADLPDIGLTPKQFADVGRIWIVACGTSWHAGLVGKYLLEEMVRTPVQVDIGSEFRY
                     RDPLIEKNDLFITISQSGETADTLAAAREAKQKGARVVSIVNVVGSTLARESDGVLYT
                     HCGPEIGVASTKAFTSQLAALYMLALHLGRVRGVLTAADGKAWLDRLVTVPALVKHVL
                     GREAEILAIAKRYYKKPDFLYLARGINFPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALIDKDMPVVVLAPRDRLYEKTVSNLMEVKARRAPVIAFVAEGERELGKIADAVFTI
                     PDVHPLLSPILFTIPLQLLAYHIAVLRGEDVDQPRNLAKSVTVE"
     gene            4373..4666
                     /gene="gatC"
                     /locus_tag="PLS35_11535"
     CDS             4373..4666
                     /gene="gatC"
                     /locus_tag="PLS35_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNI19344.1"
                     /translation="MEITKQEVEKVAKLARLALSDAETTAFSQQLNQIVGYVQKLKSF
                     STEDVEPTSTVLGQTNVFRPDQVQASLSPEQALGNAPDAEAQCFRVPKIIQES"
     gene            4692..5057
                     /locus_tag="PLS35_11540"
     CDS             4692..5057
                     /locus_tag="PLS35_11540"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNI19345.1"
                     /translation="MFRQMLRAKIHRATVTEACLEYEGSLTVDEDLLDAAGILPYEAI
                     VCSNLNNGERFMTYAMKGKRGGGEIVLNGPTARKAAVGDQIIIFCYEYYSDEEIKRHK
                     PKIILVDGKNRIARKVAKR"
     gene            5057..>5370
                     /locus_tag="PLS35_11545"
     CDS             5057..>5370
                     /locus_tag="PLS35_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNI19346.1"
                     /translation="MALTVLHKMTLAELQRKFTAGDVSATEIVRAYFLRVAHVEPKVN
                     AYLTQCKEAAVAQAERLDQSLKGWRKTTPLMAMPLAVKDNICTEGVRTTCASRMLESF
                     VPP"
     assembly_gap    5371..5470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5471..6403
                     /locus_tag="PLS35_11550"
     CDS             <5471..6403
                     /locus_tag="PLS35_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNI19347.1"
                     /translation="IRQPAAFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITKDVTD
                     AAILLGAIAGHDPRDSTSANVPVPDYLKALKRKDLKRLKVGVPAEYFADGLDPEVEQA
                     VRTAIEGLRELGADIREIKLPTTDAAVATYYVIATAEASSNLARYDGVKFGLRAGDSK
                     DLLDMYLRTRAEGFGPEVKRRIMLGTYVLSAGYYDAYYGKAQAVRTLIRREFDAAFET
                     VDLIVTPVTPTTAFKFGEKSQDPLQMYLSDIYTISANLAGLPAISLPCGFSKAGMPIG
                     LQLIGRPFEEDTLLRGAHAYEQATNWRSKKPLVR"
     gene            6422..6823
                     /locus_tag="PLS35_11555"
     CDS             6422..6823
                     /locus_tag="PLS35_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HNI19348.1"
                     /translation="MIVEVAAILVAIAFAVLVGYLVPLLIQVRKTAAEAEQLVTKMNT
                     DLPVLVTELRAMSQNLNDLTEQARGGVEHAAVLLHAVGEVGESVNQVHSLVRGSGGNL
                     LANVASVVAGLRAAKQVVTERLKEGGHHNGG"
     gene            6813..7133
                     /locus_tag="PLS35_11560"
     CDS             6813..7133
                     /locus_tag="PLS35_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNI19349.1"
                     /translation="MADNHGPSSAAVLLGFLSGAALGAVAAILLTPRTGPESREMLRG
                     YARRAEDGLRDLVGEAGERFEGAVEEGRDFIESKKSVLREAFDAGREAMRREREQFSK
                     GEQG"
     gene            7130..8563
                     /gene="gatB"
                     /locus_tag="PLS35_11565"
     CDS             7130..8563
                     /gene="gatB"
                     /locus_tag="PLS35_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNI19350.1"
                     /translation="MSAAYEVVIGVEVHAQLRTQSKLFCPCGTTFGRTANSQTCPVCL
                     GLPGSLPVINEAAVEMAVRAGLAMNGTIGVHNRFARKNYFYPDLPKGYQISQYEAPIC
                     EHGWIEIAAGGTRKRVRIRRAHLEEDAGKNLHEAGSGMSLVDLNRAGTPLLEIVTEPD
                     LSSSEEVVAYLKALRELLMYLDVCDGNMEEGSFRCEPNLSLRPVGQKAFGTKVELKNI
                     NSFKFVKDAVDYEIKRQTKVLNEGGKIYQETRLWNHERGETAVMRSKEEAHDYRYFPD
                     PDLVPLEIAADWIEQLREGLPELASTKQQRFVAEYGIPEYDAGILTSSKALSVYFDAC
                     VKLYPHPKTVSNWVMGELLRELNHAGIQADASPVTPERLVELLTMVDQGAVSLKVARD
                     IFPEMYASGKPPAQIVQDKGLTQVSDEGALVSIIDEVLKKNPAQVAQFKEGKQQVLGF
                     LVGQVMKASGGKANPGKVNELLKKTLG"
     gene            8639..9925
                     /gene="eno"
                     /locus_tag="PLS35_11570"
     CDS             8639..9925
                     /gene="eno"
                     /locus_tag="PLS35_11570"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNI19351.1"
                     /translation="MSGIRNVKARQIIDSRGNPTVEVEVLLESGAHGRAAVPSGASTG
                     EKEAIELRDGDKKRWMGKGVSKAVANVSKAIAPRLLGMEALDQAAVDHEMIALDGTKT
                     KGKLGANAILGVSLAVAKAAANETGQPLYRYLGGTNARVLPVPLMNIINGGAHADNRL
                     DLQEFMIMPVGAPRFSDALRMATEVFHTLKSLLKKKGLNTAVGDEGGFAPDLQSNEEA
                     LALIMEAIEAAGYRPGQDIALALDCAASELYEKGRYRLEAEKNPERSSEEMVSYYGKL
                     LDRYPILSIEDGLSELDWKGWKILTEKLGRRVQLVGDDIFVTNVEIFAKGIAEGIGNS
                     ILIKLNQIGTLTETLDAIELAKRSGYTAIISHRSGETEDTTIADVAVATNSGLIKTGS
                     LSRTDRVAKYNQLLRIEDELGAAAVYRGRTAVPSRT"
     gene            9931..10293
                     /locus_tag="PLS35_11575"
     CDS             9931..10293
                     /locus_tag="PLS35_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNI19352.1"
                     /translation="MIIKPNRGRDWLDWQRKLFSAGKWVGLGALFLMMGALLFGEMGI
                     SRYLHLREHAEQLDHELADLQRLNGELRADLDRVQYDSTRIEELARERLGYVRKGETV
                     YQLAPLGEKERLPTVKTP"
     gene            10290..11174
                     /gene="era"
                     /locus_tag="PLS35_11580"
     CDS             10290..11174
                     /gene="era"
                     /locus_tag="PLS35_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNI19353.1"
                     /translation="MKFGSVAIIGRSNVGKSTLLNRLLKEKIAIVSDKPQTTRTRILG
                     VAHVEGAQIVFLDTPGFHEPHHLLNRRMVRTTLETFDDADVLYVVVEATSQPGPGDLA
                     VIEHVKQAVAKQARPVVLVINKVDLVNKSRLLPLMEQYLRIFPWTEMVPVSAQTADNT
                     DRLLSVTVSLLDEGEATYNEDMITDQSMRTLAAELIREKILQQTYEEIPHSVAVEIEE
                     FVETKKLIKIGAVVIVEKESQKGILIGKHGERLKSVGTAAREDMERLFGTKVFVKLWV
                     KVRESWREDEQTLAELGY"
     gene            11281..>12323
                     /gene="mgtE"
                     /locus_tag="PLS35_11585"
     CDS             11281..>12323
                     /gene="mgtE"
                     /locus_tag="PLS35_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNI19354.1"
                     /translation="MRLVSIQRLLRRGAITNLAKMLARMHPADIANVIDHLSSQKEKR
                     EIFELVRGDVKRGQVLSELDGESITLVLSDLLPSDSAWLLKDLGSDDIAHILSVIPND
                     RAKEILALMRTEDSTEIADLLKYPKGTAGGIMTTEFFALFEDATAQEAIRRLQQATDA
                     EMVFYIYVTDKEDRLVGVLSLRQLLTVPPATPLKNIMTRDLISVAVDMDQEEVSRQVA
                     SYNLLAIPVVEKDGKLVGIITVDDVVDVIREEATEDMLKMAGAVEEDAMFKSSSMAAA
                     RVRLPWLFTNLVGSLLSGMLLWMFRYTIQEVVAIVSFIPVIAAMGGNVGLQSSTLIIR
                     GLATGLVELTDVW"
     assembly_gap    12324..12423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12424..13151
                     /gene="recO"
                     /locus_tag="PLS35_11590"
     CDS             <12424..13151
                     /gene="recO"
                     /locus_tag="PLS35_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNI19355.1"
                     /translation="HSRKWGDADRIVTCYTLRLGKLRGVARGARRLKSRLGGMIEPFT
                     LCQLDLFEKPGDSLYRISQVALDEPFAKLRSDLGLMTAAGRMVNLVNAVMAEGDAEPR
                     VFEMLESGLRTLLESRDAAWTTVLFQIRLLGITGFRPQTEQCATCGQVHRTPLPQFSP
                     LAGGMVCERCASRQPFRCTALSRGSVAFLQQALHMQPALLNRLQAAGQVRAEVESVIE
                     SYVTVVAGRRLPAVDFLTSGGPA"
     gene            13210..13566
                     /locus_tag="PLS35_11595"
     CDS             13210..13566
                     /locus_tag="PLS35_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNI19356.1"
                     /translation="MGETVLEPVDMEWVEKGVLSITWSDGHKARYPVRYLRQHCPCAA
                     CTDEWTGELRLKPDDVPMVIMLQDIQPVGRYAFQFTWSDGHDTGIYSYSFLRRLCQCD
                     VCQPVKPVEPRSRRLL"
     gene            13614..>13867
                     /locus_tag="PLS35_11600"
     CDS             13614..>13867
                     /locus_tag="PLS35_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19357.1"
                     /translation="MNVSKLASVGLWLLIGLISVACSGLPPIDQQKRLVQAGELKIQQ
                     LTPRAFAETWGDPTYTHQQFTHFFGMPDGQLIPQARMALG"
BASE COUNT         2854 a         3547 c         4499 g         2767 t
ORIGIN      
        1 agcaagaaaa ggcttgcgac gaccggcaaa agaccagtat catgacgctc tacccaggac
       61 ccctcagcca cgcatggtac aagcttcatt aaaacgctat tttctcaccg gtttgctggt
      121 gatgatcccc atctggggca ccatcctgat tctgaagacg ctgttcgtca gtctggatgg
      181 gattctcggc gacgccgccg cacggttggt aactccaggc tactacgtgc ccggtctggg
      241 aattatcgcg ctcatcctgc tcattttcat gaccggattg tttgcggcga attttatcgg
      301 ccgccaggtc gtgcggcagt gggagagttt gctcaatcgt gtaccggtgg tccgcggaat
      361 ttattcgacc attaagtcga tgatggacat tctgtcgttc gcggagcgcg agagttatcg
      421 ccgtgtggtg ttgattcagt ttccgaagaa cgggcattac tgctttgcct ttgtgaccgg
      481 tgtgacgaaa ggggaaatgc aacagctttc tccggacccg ctcgtccatg tctatgtgcc
      541 gacctcgccc aatcccacgt cgggctattt tctgctggtg cctgagcgcg aagtgatcgc
      601 ggtggatatc acggtcgaag aggccatgaa gctgatcgtg tcgggaggtc tctatacccc
      661 gacgcagccg tctggtgccg ggtcgcaggc cggtggaaaa acctggaccc cgattaagca
      721 acccgatgca ggcgtgcagg tcggttgatc gcgataccgc cgaacgagcc ctgtgtcgat
      781 tacgtatggt gagaaaccca tgactcacgc atcccacgac aatgcctcgt cttcctccat
      841 tcccgggctc ggcgtcatca tcatggcggc gggccttggc aagcgcatga aatcggcctt
      901 ggccaaagtg ctgcatccgg tggccggtcg accgatggtg ctgtacgtgc tggacattgc
      961 ctgtggcctg gctgagcagg gggtggcggt agtggtgggg catcaggggg ccgatgtgcg
     1021 caaggttgtc gaagcggtcg gcgggcaggt ggcggtcgcg gagcagacga agcagttggg
     1081 aaccggccat gcggtgctgc aagcgcgtcc ggtattcggc gatgctgcac atcgcaaacc
     1141 gacccgctat ctgattttga acggcgacac gccgttgttg acggaagcga cagtgcggga
     1201 actgctcgcg atgcatgatg cacaaggtgc ggccgtgacg ctgttgaccg ccgtgctcga
     1261 tgatgcctcg ggctatgggc gggtgattcg ccatcgtcgt gaagaatggc tgcagggtgc
     1321 cgccgacaat cgggtgcaga gcatcgtgga agacaaggat gcgtctcccg ctgagcgaag
     1381 cgtgcgtgaa attaacgtgg gtacgtatgt tgtcgacggg gagtttctct ttccggccct
     1441 cgacaaactc gatccgcgaa atgcgcaggg cgaatactat ctcaccgaca tcgtgcagat
     1501 ggccgtgcag caggggcgca ccgtctcggc attacgtctg cgggacatcg acgaaggact
     1561 ggggatcaac agccgcgtgc agttggcgga ggcggaaggc gtcattcgcc gccggatccg
     1621 ggagcgttgg ctggaagccg gcgtgacgat gctcgaccca gcctccgcct ggatcgatgc
     1681 cgacgtgacc atcgggcgcg acacgctgct gtatcccaat gtaacgctcg aaggccggac
     1741 ggtgatcggg gagaatacgg tggtgcactc cgggacccgg attacggact gcaccatcgg
     1801 ccagcgggtg gagattctgg accattgtat ttttcgtgaa tcgcaggttg aggacgactc
     1861 ccatcttgga cccttcgtcc atttgcggcc tggtgtcgtg gtgcggcgga aggggaaggt
     1921 cggcaacttt gtcgagatga agaagacgga gctcggcgaa ggctcgaagg ccaaccattt
     1981 gagttatctc ggcgatgcca tgatcggcca gggcgtgaac atcggcgccg gtacgatcac
     2041 gtgcaactac gacgggtaca agaaatttca caccgtcgtc ggagacggcg tgttcatcgg
     2101 cagcgatgtg cagttggtcg cgccggtaac ggtcggtcag ggctccgtga ttgccgccgg
     2161 ggcaaccgtg acgcaagacg tgcccgccga tgccctcgtg atcgcgcgag tgccccaggt
     2221 gacgcgtgag ggatgggctg ccaggcggcg ggccttgcag agcgggcagg tcccgcctcc
     2281 tgcgccgaag cctgtgcccc cggtgaaggc ccgcgcgagt gcaacacccg cgaaggcggc
     2341 atcgaagcca aagccgcgga cccgtgccgc caagaagaag caaccggcag ttcaacgttc
     2401 gaaacggagg taacgacacc ctatgtgtgg cattgtcggc tacgtaggaa atcaggatgc
     2461 agtcccgatt ttgttgaatg ggttgtcgaa gctggagtat cgagggtacg actcggccgg
     2521 cgtggcgatc cagcgcggag aaaagatcga catccggcgc agtgtcggga agttgatcaa
     2581 tcttcaaaac tcgctggccc agaaggcgat cgccgggatg tgcggcatcg gccatacccg
     2641 ctgggcgacg cacggaaagc cctccgagca gaatgcgcat ccgcaccgct ccgagaactg
     2701 cgtgctcgtg cacaatggca tcatcgaaaa ctacgtagag ctgaagcagc gcctcatcaa
     2761 agacggatac aagtttcagt ccgagaccga tacggaagtc gtcgcgcatc tcatcgatac
     2821 ccacatcaaa aaagacaagc tgcacctggc tgatgcggtc cgcgcgacgg cgagggagat
     2881 tcgcgggagt tatgccatcg ccgtgatctc ggagcatgag cccgggttgc tcgtcgcggc
     2941 ccgatccggt tgcccgctgg tcatcggtcg tacggcggag gcctcatttg tcggctcgga
     3001 cgtcatggcg atgttgtctc acaccaggga cgtgacgttc ctcgaagagg gcgatgtcgc
     3061 cgaggtgacg gccgggggtg tgaccttcac ggacctcgac gggcgggcgg tcacgcgcaa
     3121 gaaaaccaaa gttacctggg atgcgtctgc ggctgaaaaa agcggctatc cccatttcat
     3181 gctgaaggaa attcacgagc agcctcaaac cattctggat acgattcgag gacgctactc
     3241 gtacgagagc ggcgaggcgg atttgccgga tatcggcctg acgccgaagc agttcgccga
     3301 cgtggggcgc atctggatcg tggcgtgcgg caccagctgg catgccggcc tggtgggaaa
     3361 gtatttgttg gaggaaatgg ttcgtacccc cgtgcaggtc gatatcggga gtgaattccg
     3421 gtatcgcgac ccgttgatcg aaaaaaacga cctgttcatc acgatctcac aatcggggga
     3481 aaccgctgac acattggcgg ccgcccggga agccaaacag aagggcgcgc gcgtcgtgtc
     3541 gatcgtcaac gtcgtgggca gtaccctcgc ccgtgaatcg gacggcgtgt tgtacaccca
     3601 ctgtggccct gagatcggcg tcgcgtcgac gaaggcgttt accagtcagt tggcggcgct
     3661 ctacatgttg gccttgcacc tcggtcgggt acggggtgtc ttaaccgccg cggatggcaa
     3721 ggcctggctg gatcgcctcg tcacggtgcc ggcactggtg aagcatgtgc tgggtcgtga
     3781 agcggagatt ctggcgattg ccaagcggta ctataagaag ccggacttct tgtatctggc
     3841 ccgcggcatc aactttccca ttgcgctcga aggcgcactg aagttgaaag aaatttccta
     3901 tatccacgcc gaagggtatg cggcggggga aatgaagcac ggccccatcg cgctgatcga
     3961 taaagatatg ccggtggtgg tattggcgcc cagggaccgg ttatatgaga agacggtcag
     4021 taacctcatg gaggtgaagg cgcgccgggc tccggtgatt gcttttgtgg cggaaggcga
     4081 acgcgagttg ggcaagatcg ccgacgcggt gttcaccatt cctgatgtcc atccgttgct
     4141 gtcgcccatt ttgttcacga tcccattgca gttactggcg tatcacattg ccgtgctgcg
     4201 aggcgaagat gtggatcagc cgcgaaacct ggccaagagc gtgacggtgg aatgaggtgc
     4261 gaaggatgga ctcccgtgct cgcgcaacgc gcggcctcgg aaggccctcg ttggacgcgc
     4321 gcaattggag ccccttcctt cgcaccatac cggtttaaat gaggggtgta gagtggagat
     4381 cacgaaacag gaagtcgaga aagtcgccaa actggcgcgc ctggcgttga gcgacgcgga
     4441 aacgacggcg ttttcacaac agctgaacca gatcgtgggc tatgttcaga aactcaagtc
     4501 cttttccacg gaagatgtgg agccgacctc gaccgtgttg gggcagacca atgtgtttcg
     4561 tccggaccag gtgcaggcat ctctgtcgcc ggagcaggcg ctggggaatg cccccgatgc
     4621 ggaggcgcag tgtttccggg tgccgaaaat cattcaagaa tcttaagtga cgtaaggagt
     4681 acgagccgac tatgtttcga caaatgttgc gggcgaagat acatcgagcg acggtgaccg
     4741 aagcctgtct tgaatatgaa ggcagtctca cggtggatga ggatctgttg gatgcggcag
     4801 gcattcttcc gtacgaagcg atcgtctgtt ccaatctcaa caatggcgaa cggtttatga
     4861 cgtacgccat gaagggaaag cggggcgggg gcgagatcgt gctgaacggc cccactgccc
     4921 gcaaggccgc ggtgggtgat cagatcatta ttttctgtta cgaatattat agcgacgagg
     4981 agatcaagcg gcataagccg aagatcatcc tggtggacgg aaaaaaccgg attgcccgta
     5041 aggttgcgaa gcgctgatgg cattgacggt cctacataaa atgacgctgg cggagttgca
     5101 gcggaaattt accgccggcg acgtgagcgc gacggaaatc gtgcgcgcct actttctgcg
     5161 ggtggcgcat gtcgaaccca aggtcaatgc gtatctcaca cagtgtaagg aggcggctgt
     5221 tgcgcaggcc gaacggcttg atcagtcctt gaaaggctgg cgcaaaacga cgcctctgat
     5281 ggccatgccg ctggcggtga aggacaacat ctgcaccgaa ggggtacgca cgacctgcgc
     5341 gtcccggatg ttggaatcgt tcgtgccgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn attcggcagc cggcggcgtt ttgcggcgtc gtcgggctga agccgaccta
     5521 tggccgggtc tcgcggtacg ggttggtggc gttcgcatcc tctctcgatc agattggccc
     5581 catcacgaag gatgtcacgg atgcggccat cttattgggc gcgatcgcag gccatgatcc
     5641 acgggattcg acgtcggcga acgtgccggt tcctgattat ctcaaggcgc tcaagcggaa
     5701 agatctgaaa cggttgaagg tcggcgtccc cgccgaatac ttcgccgacg gtctcgaccc
     5761 tgaagtggaa caggccgtgc gcacagccat tgaagggttg cgggaactgg gcgccgacat
     5821 tcgcgagatc aaattgccga ccacggatgc cgcagtggcg acctactacg tcattgcgac
     5881 cgcagaggcc agttcgaatc tcgcccgtta cgacggggtg aaattcggcc tgcgggccgg
     5941 cgacagcaag gatctgctcg atatgtacct caggacgaga gccgaagggt tcgggcctga
     6001 agtgaaacgc cgcatcatgc tggggaccta tgtgttgagc gccggatact acgacgccta
     6061 ctacggaaaa gcgcaggcgg tgcggacatt gatccgccgg gaatttgacg cggccttcga
     6121 gacggttgat ctgatcgtga ctccggtgac gccgaccacg gccttcaagt tcggggagaa
     6181 gtcgcaggat ccgttacaga tgtatttgtc cgacatctat acgatctcgg cgaatctggc
     6241 cggtcttccc gccatctcgt tgccctgtgg attcagtaaa gcgggaatgc cgatcggact
     6301 gcagttgatc ggtcggccgt ttgaagagga caccctgttg cgcggggccc atgcctacga
     6361 gcaggcgaca aactggcggt ccaaaaagcc gttggtgcgg taagccggga agggagttcc
     6421 catgattgtg gaagtcgctg ctattctggt cgcgatcgca tttgcggtcc tggtcggata
     6481 cctcgttccg cttttgattc aggtccgcaa gaccgctgcg gaagcggagc agcttgtgac
     6541 gaagatgaat accgacctgc cggtcctggt gacggaattg cgggccatga gtcaaaacct
     6601 gaacgatctg accgaacagg cgcgcggtgg ggtggagcat gcggccgtgt tactgcatgc
     6661 cgtgggcgaa gtcggcgaat cagtcaatca ggtgcatagt ttggtgcgag gttccggggg
     6721 gaacttgctg gccaatgtgg cgagcgtggt agcggggctt cgcgcagcaa agcaagtggt
     6781 cacggaacgc ttgaaagagg gaggacatca caatggcgga taatcacggt ccatcgtcgg
     6841 cggcggttct gttggggttt ttgagcggtg cggcattagg cgcagtggcg gcgatcttgt
     6901 tgacgccccg aaccggaccg gagtcgcgtg agatgttgcg cggctatgcg cgtcgtgcgg
     6961 aagacgggtt gcgggatttg gtcggcgagg cgggggaacg gtttgaaggc gccgtcgaag
     7021 agggacgcga ttttatcgaa tcgaagaaat ccgtcttgcg ggaggccttt gatgcagggc
     7081 gcgaagccat gaggcgggag cgcgagcagt tcagcaaggg agagcagggt tgagcgcagc
     7141 gtacgaagtc gtcatcggtg tggaggtgca tgcgcagttg cgcacgcagt ccaagctatt
     7201 ctgcccgtgc gggactacgt ttggccgaac cgccaattcc cagacatgcc ctgtctgcct
     7261 ggggttgccc ggaagcctgc cggtgatcaa cgaggcagcc gtggagatgg cggtgcgcgc
     7321 cggattggcg atgaacggga cgatcggcgt gcacaatcgc ttcgcccgga aaaactattt
     7381 ttatcccgat ttgcccaagg gctatcagat ttctcaatac gaagcgccga tttgtgagca
     7441 tgggtggatc gaaatcgctg ccggcgggac ccggaaacgc gtacggatcc ggcgtgctca
     7501 tttggaagag gatgccggaa agaatctgca tgaagccggc agtggcatga gcctggtcga
     7561 tctcaaccgc gccgggacgc cgctgctcga gatcgtgacc gaaccggacc tgagttcctc
     7621 cgaagaagtc gtggcgtatc tcaaagcgct gcgtgaactc ttgatgtatc tcgacgtgtg
     7681 cgatgggaac atggaggaag gcagctttcg ctgcgaaccg aatctctcat tgcggccggt
     7741 tggccagaag gcgttcggca cgaaggtgga gttgaagaac atcaattcct tcaagttcgt
     7801 gaaagacgcg gtggattacg aaatcaagcg gcagaccaag gtgctgaacg aggggggcaa
     7861 gatctatcag gaaacccggc tttggaatca cgagcgcggt gaaacggcgg tgatgcgcag
     7921 caaagaggaa gcgcatgact accggtattt cccagacccg gacctggtcc cgctggagat
     7981 tgcggctgat tggatcgaac aattgcgcga gggattgccg gagttggcct cgacgaagca
     8041 acaacgcttt gtcgcggagt atggcatccc tgaatacgac gcggggattc tgacatccag
     8101 caaagccctt tctgtgtatt ttgatgcgtg cgtcaagctg tatccccatc ccaagacggt
     8161 cagcaattgg gtgatgggcg agctgttgcg tgaattgaat catgccggca ttcaggctga
     8221 tgcctcaccc gtgacgccgg aacggctggt cgaattgctc acgatggtcg atcaaggcgc
     8281 tgtgagttta aaggtggcca gggacatctt cccggagatg tatgcctctg gcaaaccgcc
     8341 ggcgcagatc gtccaggaca aggggcttac gcaggtatcg gacgaagggg ccttggtgtc
     8401 aatcattgat gaagtcttga agaaaaaccc tgctcaggtg gcgcaattta aggaaggcaa
     8461 gcagcaggtg ctgggctttc tcgtgggaca ggtgatgaag gcatccggcg ggaaggccaa
     8521 tcccggcaaa gtgaatgagt tgttgaaaaa aaccttgggc tgaggctggg atcaggtagc
     8581 gggaaatgag acgacagcag tcattgttga gtgcgagtac ctggaggaga caacgagcat
     8641 gagcggaatc aggaacgtga aggcgcggca gattattgat tcacgaggca accccacggt
     8701 cgaagtcgaa gtgctgttgg aaagcggggc gcatgggcgg gcggcggtgc cctcgggagc
     8761 ctcgacgggt gaaaaggaag cgatcgagtt gcgggatggc gacaagaagc gttggatggg
     8821 aaagggcgtg tccaaggcgg ttgcgaatgt gagcaaagcc attgcgccgc ggttgttggg
     8881 aatggaagcg ttggatcagg ccgccgtcga tcacgaaatg atcgccctgg acggcacgaa
     8941 gaccaagggg aagctcggtg cgaatgccat tcttggcgtg tccctggccg tggccaaggc
     9001 tgccgcgaat gaaaccgggc aacccttgta tcgctatctc ggcgggacga acgcgcgggt
     9061 gttgccggta ccgctcatga acatcattaa cggtggcgcg catgccgaca accggttgga
     9121 cctgcaggaa ttcatgatca tgccggtggg cgcgcctcgt ttcagcgacg cgttgcgcat
     9181 ggcaacggaa gtgttccaca cgctgaagtc ccttctcaag aaaaagggat tgaacacggc
     9241 ggtgggggat gaggggggct tcgcgccgga tctgcaatcg aacgaagagg cattggcctt
     9301 gatcatggaa gcgattgaag cggccgggta tcgtccaggc caggacattg cgctggcctt
     9361 ggattgtgcc gcgagtgaac tctatgaaaa gggacgctat cggttggaag cggagaagaa
     9421 tcccgagcgg tcttccgagg aaatggtctc ctattacggc aagttgctgg accgctaccc
     9481 catcctttcg atcgaagacg gtttgagtga attggattgg aaggggtgga agattctgac
     9541 ggaaaaactc ggcaggcgcg tgcagctggt cggcgacgat atttttgtga ccaatgtcga
     9601 aatctttgcc aaaggcatcg ccgaggggat cggcaactcg atcctgatca agctcaacca
     9661 gatcggcacc ttgacggaga cgttggatgc catcgaactg gccaagcggt caggctacac
     9721 ggccatcatt tcgcatcgct cgggcgaaac ggaagacacg acgatcgcgg acgtggcggt
     9781 ggcgaccaat agcggattga tcaaaacggg ctcgctatcc agaacggatc gcgtcgccaa
     9841 atacaatcaa cttcttcgta ttgaggatga gctgggtgct gcggctgtct atcggggccg
     9901 cacggctgtt ccctcgagga cctaacgact gtgatcatta agccgaaccg tggtcgcgac
     9961 tggttggatt ggcagcggaa gctcttttct gccgggaagt gggtagggct cggcgcgctg
    10021 ttcctgatga tgggcgcgct gttgttcggg gaaatgggta tttcccgcta tctgcatttg
    10081 cgtgagcatg cggaacagtt ggatcacgaa ctggctgatt tgcagcggtt gaatggcgaa
    10141 ctgcgcgcgg acctggaccg cgtgcagtat gactcgactc gcatcgagga gctggctcgc
    10201 gagcggttgg gctatgtgcg aaaaggggaa actgtgtatc aacttgcgcc gcttggagaa
    10261 aaggaacggc tcccgaccgt gaaaacacca tgaagtttgg cagtgtggcc attatcgggc
    10321 gttcgaatgt cgggaagtct accctcctga atcgtctgct gaaggaaaaa atcgccatcg
    10381 tctccgacaa gccgcaaacc accaggacac ggattctggg tgtggcgcat gtggaaggcg
    10441 cccagattgt ttttctcgac acgccggggt ttcatgagcc gcatcatcta ttgaaccgtc
    10501 gtatggtgcg gacgacgttg gagaccttcg acgatgccga tgtgctgtat gtggtcgtcg
    10561 aagccacgtc ccagccgggc cccggtgatc tggcggtgat tgaacatgtg aagcaggcgg
    10621 tcgccaaaca ggcgcggccc gtggtactcg tgatcaacaa agtcgatctg gtgaataagt
    10681 cgcggctgct tcctctcatg gagcagtatc tgcgcatttt cccctggacg gaaatggtgc
    10741 cggtctcggc gcagacggcc gacaataccg accggctctt gtcggtcacg gtgtcgctgc
    10801 tggacgaagg ggaagccacc tacaatgagg atatgatcac ggatcaatcc atgcgaactc
    10861 tcgcggcgga gctcatccgc gaaaagattc tgcaacagac ttacgaagag atcccacact
    10921 ccgtggcggt cgagatcgaa gagtttgtgg aaacgaaaaa attgatcaag atcggggccg
    10981 tggtgattgt cgaaaaggaa tcccagaaag ggattctgat cggcaaacac ggcgagcgtc
    11041 tcaagtcagt ggggacggca gcgcgggaag atatggaacg gctgttcggg acgaaagtgt
    11101 ttgtaaaatt gtgggtcaag gtgcgcgaat cgtggcgaga ggacgagcaa accctcgcgg
    11161 agttaggtta ttaacgacct caccattcaa ccgccggaac cggcgcgact cccggagaag
    11221 gagccgcttg ggaaagatct gtctcgcgag gccgctggtc acgggcaagc gaagtccgat
    11281 gtgcgcctgg tctccatcca acgactcctg cggcgcgggg ccattaccaa cctggcgaaa
    11341 atgctcgcgc gtatgcatcc ggcagacatt gccaatgtga tcgatcacct ctcctcgcag
    11401 aaagaaaaac gggaaatctt tgagttggtg cggggagatg tgaagcgcgg gcaagtgctg
    11461 agcgaattgg atggcgagag catcacgttg gtgctgtccg atctgctgcc gtccgattcc
    11521 gcctggttgt tgaaagacct cggttcagac gacatcgccc atattctgag cgttattccg
    11581 aacgatcgcg ccaaggagat cctggcgttg atgcggacgg aagactccac tgaaatcgcc
    11641 gacctgttga agtaccccaa gggcacggcc ggcggcatca tgaccacgga gtttttcgcc
    11701 ctcttcgagg atgccacggc tcaagaggcg atccgccgcc tgcagcaggc gaccgacgcc
    11761 gaaatggtgt tctacattta tgtgaccgat aaggaggatc gccttgtcgg ggtcttgtca
    11821 cttcgccaat tgctgaccgt tccccccgcc acgccgctca agaacatcat gacgcgagac
    11881 ctcatcagtg tcgccgtcga catggatcag gaggaagtct cgcgccaggt ggcgagttat
    11941 aacctgctcg cgattccggt cgtcgagaag gatgggaaat tggtcggcat catcaccgta
    12001 gacgacgtcg tggacgtcat ccgcgaagaa gccacggaag acatgttgaa gatggccggc
    12061 gccgttgagg aggacgcgat gtttaagtcc tccagcatgg ccgccgcacg tgtccgcttg
    12121 ccatggctgt ttaccaatct ggtgggaagc ctgctgtccg ggatgctgct ctggatgttc
    12181 cggtacacga ttcaggaggt cgtcgccatt gtcagtttca tcccggtcat cgcggcgatg
    12241 ggaggcaacg tcgggctgca gtcctctacg ctcatcattc gagggctggc gaccggcctc
    12301 gtcgagctca ccgatgtctg gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnntgcacag ccggaaatgg ggcgacgccg accggattgt cacgtgttac acgctgcggt
    12481 tggggaaact gcgcggcgtg gcgcgagggg cgaggcggtt gaagagccgc ttgggtggca
    12541 tgatcgagcc gtttacgctt tgccagctgg atttgtttga aaagcccggg gattccttgt
    12601 accgcatttc gcaggtcgcg ttggatgaac cgtttgcgaa acttcgcagc gatctcggat
    12661 tgatgactgc cgccgggcga atggtcaatc ttgtgaacgc cgtcatggcc gagggcgatg
    12721 cggaaccgcg cgtctttgag atgttggaat cgggcctgcg tacgttgctg gagagccggg
    12781 acgcggcctg gaccaccgtg ttgtttcaga ttcgtttgct gggtatcact ggatttcgtc
    12841 cgcaaactga acagtgcgcg acctgcggac aagtacaccg aacgccgctt ccgcaatttt
    12901 cgccgctggc cggaggaatg gtctgtgagc gttgtgcgag tcgccagccg tttcggtgca
    12961 cggccttgtc gcgtggcagc gtggcctttc tccagcaggc cctgcacatg cagccggcgc
    13021 tgctgaatcg attgcaggcg gcagggcagg tgcgggcgga agtcgaatcg gtgattgaaa
    13081 gttatgtcac ggtagtggca ggtcggcgtt tgccggccgt ggattttctg accagcggcg
    13141 gacctgctta agtgcgacgg tggtgttcgc aaggtctcct attcaaggcg tgagggagcg
    13201 ttgatcgcta tgggtgaaac ggtgctcgag cccgtcgata tggaatgggt cgagaagggt
    13261 gtgctgagca ttacgtggag tgatggccac aaggcccggt atcccgtccg ctacctccgg
    13321 cagcattgtc cgtgcgccgc gtgtacggat gaatggacgg gagaacttcg ccttaagccc
    13381 gacgatgtgc cgatggttat catgttgcag gacattcagc cggtggggcg gtatgccttt
    13441 caatttacct ggagtgatgg gcacgatacc ggaatctact cgtactcgtt tcttcgccgg
    13501 ctctgccagt gtgatgtgtg ccagccagtg aaaccggtgg aaccgcggtc tcgacggttg
    13561 ctgtgatgag atgcggtcgc tgtttacaga tgaacaggga aaggcgggat cggatgaacg
    13621 tctctaaact ggcatccgtg ggtttgtggc tgctgatcgg actgatttct gtggcttgtt
    13681 cgggtctccc tccgattgat cagcagaaac gattggttca ggccggagag cttaaaattc
    13741 agcaattgac gccgcgggcc tttgccgaaa cctggggcga tccgacatac acccatcagc
    13801 agttcaccca ttttttcggc atgcccgatg gtcaactgat tcctcaggcg cgcatggcgc
    13861 ttggcga
//
LOCUS       DAOQEW010000100        13815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1265_length_13815_cov_7.333939, whole genome shotgun sequence.
ACCESSION   DAOQEW010000100 DAOQEW010000000
VERSION     DAOQEW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13815
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1265_length_13815_cov_7.333939"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLS35_11605"
     CDS             <1..1059
                     /locus_tag="PLS35_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19358.1"
                     /translation="RISDTLPRWHTLMEFQSINLLNNTPTGGSAATGGVPTSSTFFRE
                     AYLDIRPVQSWAPNVNYFRMGIFRMPFGIFTETSGGLRDVISSPYLTSVGSGVGNRTG
                     SAGTVEFLQERDYFLDMRGKLFNRLEYVVGIMNNNNYEANATGSNAPKSFYSRVRLFG
                     SDVSFISFTTIQGESNNANTNINGRGKGAFDRYGLDFRYTSKILPGFMVQGEIWQGHD
                     GANQTTVGRPANGACDVQAICGGSGAPGVQRRTWYVLAKYLITDGPFENFEPVIMYEQ
                     FDPNTRIGNDLYTRTIVGLSYYFENFPPKVQSKLQVNYEFRHHSGNGPNNVVDPTRGV
                     GDPFAQNAFLVQWQIRFM"
     gene            1128..1667
                     /locus_tag="PLS35_11610"
     CDS             1128..1667
                     /locus_tag="PLS35_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNI19359.1"
                     /translation="MKIYRGAIAAVLIGLVLNGCGPQRVFSPQVLDGVDPNFDFTAWR
                     MVPNAKAGKKVQLGGRILQAPQKDGLVTMVLTQLPIVEHPAYGPTDTERGRGEFLVYF
                     NGKVPTNHLQPGNRVIAIGTTQNAAVVAIEDSQRSLPALTAQCLHIWNTGGKEIADFP
                     NFGAGYQPLEEETYCAESR"
     gene            2002..2976
                     /locus_tag="PLS35_11615"
     CDS             2002..2976
                     /locus_tag="PLS35_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19360.1"
                     /translation="MPVASMVTIPYPRLALRRSLCLVLLMYLTSACSLLTGGKVLFDQ
                     QDSQVGVQTDPTVHRGPAPTENNHPAQLTTEEVRVLLGSLMVSGWSGTLVGILERPRP
                     IPVFTELELNRIAAPVAAAFQQATPSERVFFSLPKSQTTYSEERTTGALFLRGRYLHV
                     VLTDHSAFTRADTAGGEVKDLRDTKGMKLWVTAPATAATVPDAEEPRWAHFETVHVSL
                     NAKEVIAARNRPASPSADVSPSISLHPSEAVGAGAKSNGPADTTGNQTDELRLQVREL
                     TTSNLELRNRLDTQLKQMQALQDQLTKLQQDLDSARAKSKTPRRSTTP"
     gene            complement(3490..4608)
                     /locus_tag="PLS35_11620"
     CDS             complement(3490..4608)
                     /locus_tag="PLS35_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNI19361.1"
                     /translation="MLSHGQKHGWRRRAVVVGLAGILATAESLAVLPAGVARAENAGP
                     TASLAIQEGLVQYNPQTQVAGSLRVQGSDTMSLLMSRLAGEFQRRQPNVSINVSGGGS
                     TKAVNEFVQPPAKLSGKITLKEERPTYVSLVASSRELLDAEVKQFVAQHGYEPTAIPV
                     AVDAVALYVHRDNPIQGLTLDQVDAMFSTTHNRGLKTSLSQWGALGLQDGWEKAPVRL
                     YGRDRRSGTRAFFQEHCLAGGEFAPSVREEPGAASVILDLTRDQLGIGYSGLGLETSN
                     VRIVPLAENQGMPFIAPTATTVSDQTYPLRRMLYLYVDKSPKAALAPAVQEFLTFVTS
                     REGQEAVMKAGFFPLPMNQLKKNFLALGLSGQETPLPN"
     gene            complement(5113..5496)
                     /locus_tag="PLS35_11625"
     CDS             complement(5113..5496)
                     /locus_tag="PLS35_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI19362.1"
                     /translation="MVTVSELMTKKLVTVPVGTTTAEAAKVMNERHVGSVFIEQNNRV
                     VGIVTESDIVRKVVGENKPVHFVPVESIMSSPVISLDERRSITEAADLMQCHHTRHLG
                     VLQSGAIVGVLSVRDLLQPVAVDEF"
     gene            complement(5703..6278)
                     /locus_tag="PLS35_11630"
     CDS             complement(5703..6278)
                     /locus_tag="PLS35_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI19363.1"
                     /translation="MIVLRPFALVGLLAVAAPFLFDGCASKAGTSGGATVTPSKPRVE
                     ERIAAAPVQEIPPAPEPAPVPLRSVEMAARNATGEQNIPFPDVLFDFDQYVLRDDALN
                     AVEANAKRLKDNRVTKVLLEGRCDEIGTAEYNLVLGERRALSVKRYLESLGMSQVQVD
                     VTSYGKDRPLCLQHNPVCWQKNRSVHFVVKE"
     gene            complement(6353..10042)
                     /locus_tag="PLS35_11635"
     CDS             complement(6353..10042)
                     /locus_tag="PLS35_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNI19364.1"
                     /translation="MTDHHANRPANRLIRETSPYLLQHAYNPVDWYPWGPEALAQAAA
                     LKRPILLSIGYSSCHWCHVMERESFENEAIAALMNQHFICVKVDREERPDLDEIYMQA
                     TLALNRNQGGWPMTVFLTPDQKPFFAGTYFPPTDRYGRPGFPTLLKKIAEYWEKDHDG
                     VVAQAVNLTARLQEGAHAPSPTTVGEAELDMAVTQFAEDFDARQGGFGGAPKFPPATG
                     LSLLLHCYQRTKDPHTLTMVRKTLDAMAAGGIYDHIGGGFARYSTDERWLVPHFEKML
                     YDNALLARVYVEAYQVTADETYRRVACETLDYILKEMTSPEGGFYSATDADSEGVEGK
                     FFVWTPEDVRAAVDSDEEARRFCAYYDVTAGGNWEHTNVLHTAKSVEAVAKELSITAG
                     ELTETITRLRPKLYAVRAKRIAPGLDDKVITAWNGMMISAMAEAGRVFDIPRYREAAE
                     RACDFLLTTLSTADGRLLRTYRAGTAHLNAYLEDYAYFAEGLIDTYEAGGEERYLLAS
                     VRLAERMLADFADSQHGGFFTTATGHEALIMRSREGPDGATPSGNAVAASVLARLSYH
                     FAREDFREAATAAVRAYGRQIARYPRAFAKSLIVVDFLMSGPAEIALVGRLGDQGMES
                     LRAAVNRTYLPSRVLAHRDSDQSATSHPLLQGKTLIDGKAAVYVCRNFACGRPITDPK
                     EFPSLLDPRQQASSPPASEQKILSGTLYPGHATVQGTAAYAARHIHESNGALAHGFAL
                     FGATGLTASRLGFGTYRVGQREAEHREALRSALRQGCNVIDTSTNYMDGESEQLVGSV
                     LREMMRAGDLTREGVIVVSKIGYVQGQNLTQAQAREKSGKPYPDMVKYGDDIWHCIHP
                     EFLADQLTLSLDRLGLATLDVCLLHNPEYFLSHATRLGGDESRDLPALRQEFYARLQR
                     AFEYFETQVQAGRLRGYGVSSNTATSAPDDPGATSLSHMIDAAKLAATTVGAPSHHFQ
                     VLQCPMNVYESGAALIKNTGIHNGSTLLEEAMRERIAVLVNRPLNAMPVQRGGVIRLA
                     DVSVPAPEADFEAQRKKVAALEEEYRNSLAPAVAHSGQGMLPADFFRWADELTRIRGQ
                     VQGLEHWEQIEQQMIAPHVNQVLRALAEAFTGNVAEQWESWRDRYVPELLALLRILHR
                     EASEKSRLRSDELHHTMNPLLPEQRRTATLSQKALWVLASTPGVTCVLNGMRTPAYVE
                     DALTILQWPPLPEWRSVYDRCAGKK"
     gene            complement(10192..10416)
                     /locus_tag="PLS35_11640"
     CDS             complement(10192..10416)
                     /locus_tag="PLS35_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19365.1"
                     /translation="MKRALFAFVAAAILVAFTAPSFAGDDKKKEEKKGGHFSQTLFGD
                     DKKKEEKKGGHLSQTVFGEEKKKDEKKGGH"
     gene            10808..12250
                     /locus_tag="PLS35_11645"
     CDS             10808..12250
                     /locus_tag="PLS35_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HNI19366.1"
                     /translation="MTTDDIGPYSNYRLTVRLALLNKPGIFAKVAALLAEEGANLGAV
                     DIVSANAERMIRDITFDVQNEAHGDRVLERLEALPEVNVLSASDRIFLLHLGGKIRVQ
                     SKVPVTTRNVLSMIYTPGVGRVCKAIAKDPVKAYAFTSKSNSVAVVTDGSAVLGLGNL
                     GPAAALPVMEGKVMLFRELAGIDAWPICVSTQDPEEIIRIVRGIAPGFGGINLEDISA
                     PRCFEIERALKTSLDIPVMHDDQHGTAVVLLAALTNALKVVGKRMEDVRIVVNGLGAA
                     GTACCRILLAAGACHLVGCDKEGIVLMGEAEELRACRTDLQACIRRDQPRGTIHDALR
                     GADVFIGLSVGNVLEKADLDNMNSDRIVFAMANPDPEIDPKIGDEVSRIFATGRSDYP
                     NQINNALSFPGIFRGALDVQASEINEAMKLAAAQAIAECVPTEALSEDYIIPSVFDRD
                     VVPRVAKAVAAAARTTGVARRRIKIEDDPR"
     gene            12418..13677
                     /locus_tag="PLS35_11650"
     CDS             12418..13677
                     /locus_tag="PLS35_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HNI19367.1"
                     /translation="MTPTTIQLIGTLLFAVAILHTFATAFFEHLAHTRPAHAGLWHLL
                     GEVEVAFGFWALVLIVAMFVTDGAAAATHYLDSRSFTEPLFVFAIMVIAGTRPILQTA
                     MTGVRIVSSAIPLPQGMRVYLTVLALVPLLGSFITEPAAMTLAALLLRDVFFRHGCST
                     NLKYATLGVLFVNVSIGGTLTPFAAPPVLMVAGAWQWDIAFMLGTFGWKAALAVLINA
                     FGATMLFRRELSRISTAIDQREENRAPLALVITHLALLAMVVVFAHHPAMFLGILLLF
                     LGIAQAYEQYQDRLILREGLLVSFFLAGLVVLGGQQQWWLQPILLGMGADAVFVGATI
                     LTAFTDNAALTYLGSLVEGLSDAFKYALVAGAVTGGGLTIIANAPNPAGLAILRTYFD
                     DEAVHPLRLFLAALPPTLIAAAMFWCL"
BASE COUNT         2936 a         4148 c         3892 g         2839 t
ORIGIN      
        1 cgtatcagcg acaccctgcc gcgatggcac accttgatgg agtttcaaag catcaatctc
       61 ttgaacaaca caccgacagg aggcagcgcg gccactggcg gagtgccgac ctcgtcgaca
      121 ttctttcggg aagcctacct cgacatacga ccggtgcagt cgtgggcgcc caacgtgaat
      181 tatttccgca tgggtatctt tcgaatgccc ttcggcatct tcacggaaac ctccggcggc
      241 ttgcgcgatg tcatcagctc tccctacctt acctcggtgg gctctggggt cggcaatcgt
      301 accgggtcgg ctggcaccgt cgagtttctc caagagcggg attacttcct cgatatgcgc
      361 gggaaactgt tcaaccggct ggagtatgtg gtcggtatta tgaacaacaa caactatgag
      421 gccaacgcga ccggatccaa cgcgccgaaa agcttctact cccgcgtccg attgttcggg
      481 agcgacgtgt catttatcag tttcaccacg attcaaggcg agtcgaacaa cgccaacacc
      541 aacatcaatg gtcgcggcaa gggagccttc gatcgctatg gactggactt tcggtatacc
      601 tcgaaaattc ttcctggctt catggtgcag ggagaaatat ggcaggggca tgatggggcc
      661 aatcagacga cagtcggacg cccggccaac ggtgcttgcg atgtccaagc catctgcggc
      721 ggctccggcg caccgggggt acaacgacgt acctggtacg tactggccaa atatctcatc
      781 actgacggcc ccttcgaaaa cttcgagccg gtcatcatgt atgaacaatt cgacccgaat
      841 accaggatcg gcaatgatct ctacaccaga accatcgtcg gcttgagcta ctatttcgaa
      901 aactttccgc cgaaggtgca gtccaaactg caagtcaatt acgagttccg ccatcactcg
      961 ggcaacgggc ctaacaatgt cgtcgatccg acgcgcggtg tgggcgaccc tttcgcccag
     1021 aatgccttct tggtacagtg gcaaatccga ttcatgtaac gggatgactg caactgcggc
     1081 aacaactgtc taaccgattc ggtctcgagc aaagagaaag gctagcaatg aagatatacc
     1141 ggggagcgat cgcggcggtg ctgatcggtt tagtgctcaa cggatgcgga ccacaacgcg
     1201 tcttctcacc tcaagtgctg gacggggtgg acccaaattt cgactttacg gcctggcgca
     1261 tggtgccgaa tgccaaggcg gggaaaaagg tgcaactggg tggtcgcatc ctgcaggctc
     1321 cgcaaaaaga cggcctggtc accatggtgc tgacccaatt gccgattgtc gaacacccgg
     1381 cctatggtcc gacggatacg gaacggggga ggggtgaatt tctggtgtat tttaacggca
     1441 aggttcccac aaaccatctg cagcccggga atcgcgtcat tgccatcggg accacgcaaa
     1501 acgcggcggt ggtcgctatc gaggatagcc aacgcagtct gcccgctctc acggcccagt
     1561 gcctacatat ctggaacacc gggggaaaag agattgctga tttcccgaac tttggggcag
     1621 gatatcagcc actcgaggag gagacgtatt gcgccgaatc ccgctgacaa gccctgaaag
     1681 tattccacct tcacgagggc ggcttcgcac acttgatgtg accgccgctc tcgtgctggt
     1741 gtgggcatgc agcgcctgcg gagccggcgt gaccctagtt cgcgacacgc cgacaggagg
     1801 gctcgtcacg taccccttcc aaaatgaagt cgatgtcctc acttccagcg agcggcgtga
     1861 cgcgatggag ttgatcaacc gcaaatgccc ggctgggttc aaagttcaga aagaaggcga
     1921 aataccaaag gtgagccgcg ctgcggatcg agcctggcga ggacaaatgg gaggggacag
     1981 agtatgggga attcagttca catgccagta gcgtcgatgg tcaccatacc ctatccccgg
     2041 ctggcattgc gccgcagcct ctgcctcgtt ctcctcatgt acctgacctc cgcatgctcg
     2101 ctattgaccg gaggaaaggt gctgttcgac caacaggaca gtcaggtggg agtgcaaacg
     2161 gacccgactg tccatcgcgg acctgccccc acggaaaaca accatcctgc gcagctgacc
     2221 acggaggagg tgcgcgtgct tctcggttcg ttgatggtga gtggttggag cggaacgctg
     2281 gtgggcatcc tggaacggcc ccgtccgatc cctgtcttca ctgagctgga attgaatcgg
     2341 atcgccgcgc cggtcgcagc cgcttttcaa caagcaactc ccagcgagcg cgtgtttttt
     2401 tccttaccta aatcacagac gacctatagc gaagaacgga ccaccggtgc gctctttctt
     2461 cgaggacgat atcttcacgt cgtgctcacg gaccactccg ccttcacgag ggcggacacg
     2521 gccggagggg aggtgaagga tctccgagat acaaaaggaa tgaagttgtg ggtcaccgct
     2581 ccggcaacag cagccaccgt acccgatgcg gaagagccac gctgggctca ttttgaaaca
     2641 gtacacgtct ccctgaatgc caaggaggtc attgctgcca gaaaccggcc tgcttctccc
     2701 tcggctgacg tttcaccctc aatatcgctt cacccatcag aggcagtggg agccggcgca
     2761 aagagcaacg ggccagccga cacgaccggc aatcagaccg atgagcttcg cctgcaagtg
     2821 cgtgagctga cgacctcaaa tctggaactt cggaatcgtt tagatactca actgaaacag
     2881 atgcaggcgt tgcaggatca attaacaaaa cttcaacaag accttgactc cgctcgagca
     2941 aaaagcaaga cacctcgccg atctacaacc ccatagccat ccacaccttt actatcagcc
     3001 atctacgcat aatccgttgt tggaatcctc gctcatatcg tgagacacct cggtgtttct
     3061 cctcgagagg agtaccggcc cagtgagcag cgattgcgcg atccaataag gcgagccgct
     3121 cacctcttcg catccgtgat ccgctgaatc acgccaacct gacggctgcg catgttcggc
     3181 gcctcacaat ctacacattg gcgtaacatc cggtatcgtc atgtgctgcg ttacccgctc
     3241 ggccgctcgc atcaggccga agttccggcc ccctaggtca gccattctgt cgggcgagga
     3301 cggtggtgca acgcacgagc aataagtatt caggctatcg ataattctcg aaggcgggta
     3361 gcctctctcg aatgctggcg ggagacgcag ttggtcagcg aggaaaggat gagaacggtt
     3421 tgcaccgcag gcatcgagga atagtctcga tgacctgcgg cagtagccat tatgcgaaac
     3481 gacggtggat cagtttggga ggggagtctc ttggccggaa aggccgagcg ccagaaagtt
     3541 ctttttcagc tggttcatcg gaagagggaa aaatcccgcc ttcatcacgg cttcctggcc
     3601 ttcacgactc gtcacaaacg tcaggaactc ttgcacagca ggggcgaggg cagccttggg
     3661 agatttatcg acatacagat agagcatccg gcgaagggga tacgtttggt cggaaacagt
     3721 tgtcgccgtc ggtgcgataa atggcatgcc ctgattctca gcgagcggaa cgatccgcac
     3781 attggacgtt tcaaggccca atccactgta tccgataccc aattgatcgc gcgtcagatc
     3841 aagaatgacc gatgcggcac ccggttcctc ccgaacagac ggtgcgaatt cgccaccggc
     3901 taagcaatgt tcctgaaaga acgcgcgcgt cccggatcgc cgatctcgac catagagtct
     3961 gacgggagcc ttctcccaac cgtcttgcag tcccagagcc ccccattgcg acaggctggt
     4021 ttttaatccg cggttgtgcg tggttgaaaa catggcatcg acctggtcga gcgtgaggcc
     4081 ttggatcgga ttatcacgat ggacatacag tgccacggca tcgactgcga cggggatggc
     4141 ggtgggttca tatccatgct gggcgacaaa ttgtttcact tcggcatcaa gcaactcgcg
     4201 tgaggacgcc accagggaga cataggtcgg tcgttcttcc ttgagggtaa ttttcccgct
     4261 caatttggcg ggcggctgga cgaactcgtt cacagctttg gtcgatccac ccccgctcac
     4321 attaatcgag acattcggct ggcgtcgttg aaactcacct gcaagacgac tcataagcag
     4381 agacatcgta tcggaacctt gaacccgaag gcttcccgcc acttgagtct gcggattgta
     4441 ttgtaccaat ccttcttgaa tggccagact cgccgttggt ccggcgttct cggcccgtgc
     4501 gactcccgcc ggaagcacag ccaacgactc agcagtagca aggattcctg ccaatcccac
     4561 aaccactgct cgccgtcgcc atccatgctt ctgtccatga cttaacatgc tcttctcctc
     4621 cgctagagtg gtggtatcgg ctataactgt atgtatccta ctaaccgatg attgctgctt
     4681 catcactggc atgttacgga tgtgttaaat cgtcccctag agtccgatgc cagcgggatg
     4741 gaccccgttg gagcgtgaat gcagcctgac ctgatcgtgc tcggtcttaa ccaccgactg
     4801 aacggatcct cttcgacaag cctcgactct ctacgcaagt agcacttcga tagcacgaag
     4861 acaaggccac tcccggcaga aactgctaca ccgttgccct cgcatgtcga gacggtattc
     4921 gcagccatac cagccaaggg atgtctccga tgtcgtgagg aacactgtcg gctgagggct
     4981 gtgactgaca caaggaagag tgaacgggag agaggtttcg gagcaacgtc ttgcggctgt
     5041 cggagctccg accacacacg cgatcggagc gctcagccaa acgacgaacg ccaatgcact
     5101 gtgaaaaaac gattagaact cgtccaccgc gaccggctgc aagaggtcgc gcaccgagag
     5161 caccccgacg atcgcgccgc tctgcaacac gccgagatga cgagtgtgat ggcactgcat
     5221 gagatcggca gcctcggtaa tcgatcgccg ctcgtcgaga ctgatgaccg ggctgctcat
     5281 gatcgattcg accggaacaa aatgcaccgg tttgttttct ccgacaactt ttcgaacaat
     5341 gtcggactcg gtcacgatgc ccacaacacg attgttctgc tcgatgaaca cactaccgac
     5401 atgacgctca ttcatcactt tagcggcctc ggccgtcgtt gtgccaaccg gtaccgttac
     5461 cagtttcttc gtcatgagct cgctgacagt caccatatcc gatcctcctg gttagatggg
     5521 ttgactcaga aacacgccct tctgagtctc taactcgaag gatagaacgg gcatattgaa
     5581 agcgggttat gggcaggtaa aatctttgtt acgactgacg gagagtcgga agaacgtcga
     5641 accaagcgcg gcatgggcca cgtacgccca tgccgccgtc gtcacaacca gatgggggga
     5701 gtttattctt tcacgacgaa gtgcacactg cgattcttct gccaacagac cggattatgc
     5761 tgcaaacaca ggggacggtc tttgccataa ctcgtgacat ccacctgcac ctgactcatg
     5821 ccgagtgatt ccaggtaacg cttcacggaa agggcacgac gttcgccgag caccaagttg
     5881 tattccgcgg tgccgatttc gtcacaccgg ccttccaaca acactttggt gacccgattg
     5941 tcttttaatc gtttggcatt cgcctcgacg gcattcagcg catcgtcccg cagaacatac
     6001 tgatcgaaat cgaacaacac atcagggaag gggatattct gttcccccgt cgcattacgg
     6061 gcggccattt ccactgagcg caagggaacc ggcgccggtt cgggagccgg aggaatttcc
     6121 tgaacaggag cagcagcaat ccgctcctcc acacggggtt tggaaggagt tacggtggct
     6181 cctccactgg ttcccgcctt gctcgcgcag ccgtcaaaca gaaagggagc ggcgacggct
     6241 aacagtccaa ccaaggcaaa cgggcgcaaa acgatcatga aaacctccgg cgctaggtga
     6301 gttcgtggac cagacgaggc acagaataat caaaagatgc gaacctggca agttacttct
     6361 tcccggcaca acgatcgtag accgatcgcc actccggcaa ggggggccac tgcagaatcg
     6421 tgagcgcatc ctccacatag gccggcgtgc gcataccgtt gagcacacag gtgacacccg
     6481 gcgtgctggc gagcacccag agggcctttt gcgacaaggt ggccgtgcgt cgctgttccg
     6541 gtagaagggg attcatggtg tgatggagtt catcggagcg gaggcggctt ttttctgatg
     6601 cctcccgatg cagaattctc aggagcgcca acaattccgg cacgtatcgg tcccgccagg
     6661 actcccactg ttcagcgacg tttccggtaa acgcctcggc cagcgcacga agcacctggt
     6721 tcacgtgcgg cgcgatcatc tgctgttcga tttgttccca atgctcgagc ccctgcacct
     6781 ggcctcgtat tcgagtcaac tcatcggccc atcgaaaaaa gtcggcgggt agcatgccct
     6841 gaccgctgtg cgcgactgca ggggcgagac tgttgcgata ttcctcctcc aatgcggcga
     6901 ctttctttcg ctgcgcctcg aaatcggcct ctggagccgg caccgagacg tccgccaatc
     6961 gaatcactcc tccgcgctgc acgggcatcg cattgagtgg acgattgacc agcacggcga
     7021 tccgctcgcg catggcttcc tccagcagcg tgctgccgtt gtgaatcccg gtgtttttga
     7081 tcagagctgc gcccgactcg tacacattca tcggacattg gagcacctgg aagtgatggg
     7141 acggggctcc aacggtcgtg gccgccaact tcgccgcgtc gatcatgtga gacagggaag
     7201 tggcaccggg atcgtcggga gctgacgtgg ccgtattcga tgagacacca tagccacgca
     7261 agcgacccgc ctgaacctgc gtctcgaaat actcgaaggc ccgctgaagt cgcgcataaa
     7321 actcctggcg taacgcaggc aggtcgcgcg actcatcgcc gccgagacgc gtggcatggg
     7381 acaggaaata ttccggatta tggagcaggc acacatcgag cgtggctaac cccagccggt
     7441 ccaacgacag cgtgagttgg tccgccagaa actccgggtg gatgcaatgc cagatatcgt
     7501 caccatactt gaccatgtcg gggtagggtt tgcccgattt ttcccgcgcc tgggcctgcg
     7561 tcaaattctg gccctgtacg tacccgatct tcgacaccac gatgacgccc tcgcgggtta
     7621 gatcgcccgc gcgcatcatc tcccgcagga ccgatcccac caattgctca ctctcaccat
     7681 ccatgtaatt ggtcgaggtg tcgatcacat tacagccctg gcgcaatgcg ctgcgtaagg
     7741 cctcacgatg ttcggcctcc cgctggccga ctcgatacgt tccgaatcct aatcgactgg
     7801 ctgtcagtcc agtcgcacca aacagggcga atccatgcgc gagcgccccg ttgctctcgt
     7861 gaatgtgtcg cgcagcataa gccgctgtgc cctgcacggt cgcatggccc gggtataacg
     7921 ttccgctcaa gatcttctgc tccgatgcgg gaggtgagga ggcctgttgc cggggatcaa
     7981 gcagagaggg aaattctttc ggatcggtga tcggccggcc gcaagcaaaa ttccggcaca
     8041 catacacggc tgccttgccg tcgatcaacg tctttccctg caggagcgga tgactggttg
     8101 cgctctgatc tgagtcgcga tgagcgagca cgcgactggg aaggtaggtg cgattcacgg
     8161 cggcccgtaa agactccatc ccttgatccc cgagccgccc gaccaacgca atctccgcag
     8221 gcccactcat gaggaaatcg accacgatga ggctcttggc aaatgcccga gggtagcgcg
     8281 caatctgacg gccataggcg cgaacggcgg cggtcgcggc ctcccggaaa tcctcccgcg
     8341 caaaatgata ggacaatcga gccagcaccg aggcagccac cgcgttgccg ctgggcgtgg
     8401 ctccatctgg tccctcccgg ctgcgcatga tcaacgcctc atggccggtg gcggtggtga
     8461 agaacccacc gtgctggctg tcggcaaaat ccgccagcat ccgctcggcg aggcgaaccg
     8521 acgcgagcag atagcgttcc tcgcccccgg cttcataggt atcgatcaag ccttcagcaa
     8581 aataggcgta atcttccaga taggcattca ggtgggcggt acccgcacga tacgttcgaa
     8641 ggagccggcc atcggcagtc gagagtgtcg tcagcagaaa atcgcaggcc cgttcggccg
     8701 cttcgcgata acggggtatg tcaaacaccc gtccggcttc cgccatggcg ctgatcatca
     8761 tcccgttcca cgccgtgatc accttatcat cgaggccggg cgcaatgcgc ttcgcacgca
     8821 ctgcgtaaag cttcggtctg agacgggtga tggtttcagt taactcgccg gcagtgatgc
     8881 tcagttcctt ggcgaccgcc tcgacgctct tcgcggtatg gagcacattc gtgtgttccc
     8941 agttaccccc cgccgtcaca tcgtaatagg cgcagaagcg gcgcgcctct tcatcactgt
     9001 ccaccgccgc tcgcacgtct tccggcgtcc acacaaagaa cttgccctcg acgccttcgg
     9061 aatcggcatc ggtggcggag taaaaccctc cctcgggtga cgtcatttcc ttgaggatat
     9121 aatccagcgt ctcgcaggca acccggcggt aggtctcgtc tgccgtgacc tggtaggctt
     9181 cgacatagac gcgagccagc aacgcattgt cataaagcat cttttcgaag tgcggaacca
     9241 gccaacgttc gtcggtcgaa taccgagcga aaccgccgcc gatgtgatcg tagattccgc
     9301 ccgccgccat ggcatccaac gtcttgcgca ccatcgtcag cgtatgggga tccttcgtgc
     9361 gctggtaaca atggaggagc agggacaacc ctgtggccgg aggaaatttt ggggcgccgc
     9421 cgaagccgcc ctgtctcgca tcaaaatctt cagcaaactg ggtcacggcc atatcgagtt
     9481 ccgcttcgcc gactgtcgtc ggagaaggcg cgtgagcccc ctcttggagc cgtgccgtca
     9541 gattcaccgc ctgcgcaacc acgccatcgt ggtccttctc ccagtactcg gcgatcttct
     9601 tcaacagggt cgggaagccg ggacgaccgt agcgatctgt cggaggaaaa tacgtgcccg
     9661 caaaaaacgg tttctggtcc ggagtgagaa acaccgtcat cggccagcca ccttgattgc
     9721 ggttcaacgc caaggtcgcc tgcatgtaga tttcatccag atccggccgc tcttcgcgat
     9781 cgaccttcac gcagatgaag tgttggttca tcagggcggc aatcgcctcg ttttcaaacg
     9841 actcccgctc catcacgtga caccagtgac aagaggaata cccgatggag agcaggatgg
     9901 ggcgcttcag tgctgcggcc tgagccaagg cttccggccc ccagggatac caatccacgg
     9961 ggttgtacgc atgctgtaag agatagggac tggtttctcg aatgagccga ttggcggggc
    10021 gattcgcgtg gtgatcagtc attcgcgaac cgtaacacgg gctcagtgaa tgggtcaatg
    10081 aagcggggag ccgggcaggg aaggggcgga agagataaaa aaattggccc gcgccggcaa
    10141 tcccgggtcg cgagccaact ttatactacg acagaacgtc gaaagcgggg ctcagtggcc
    10201 gcccttcttc tcgtccttct tcttctcttc gccgaacacg gtctgggaca ggtgtccacc
    10261 cttcttctct tccttcttct tgtcgtcacc gaagagggtc tgggagaagt ggccgccctt
    10321 cttctcttcc ttcttcttgt catctccggc gaaagacgga gcggtgaacg caacgagaat
    10381 ggctgccgct acgaatgcga atagagcacg cttcatggaa acccctcctt gaaaggttga
    10441 gaatataagt gcccacacca caagggcgcc cgagggtagc caacctgtca gaaaaagtca
    10501 acgaaactct ttgcgtatat ttcaccagcc tcgtcatcca acggatgcgt cacggcctcc
    10561 tcctgccggg gatgtctgga gcgctttact tttcgagacg ttaacggatc tcgacagcct
    10621 atggttcacc gcgaacccat caacgctcga ccttcacgaa gtgccaccct gatggggcat
    10681 ttcttcggga gatagacgaa gtggaaggag gccaatgcag gaaacgtcat gcttcaccag
    10741 aagctcagat ccgtccgcgc tgtgatacac tcttcataag taatccacac ggtctcggat
    10801 caatcctatg accaccgacg acatcggccc gtactccaac taccgcctga ccgtccgcct
    10861 cgcgctcctc aacaaaccgg gcatcttcgc gaaagtggcc gccctgcttg cggaggaagg
    10921 agccaacctg ggggcggtcg atatcgtgtc cgccaatgcg gagcgcatga tcagggacat
    10981 taccttcgat gtgcagaatg aggcgcatgg cgaccgggtg cttgagcgcc tggaagcgct
    11041 gccggaagtg aatgtcctgt cggcctcaga ccggatcttc ctcctgcacc tgggcggaaa
    11101 aatcagagtc cagagtaaag tcccggtcac gacccgcaac gttttgtcca tgatctatac
    11161 gcccggcgtt ggacgcgtct gcaaagccat cgccaaggat ccggtaaaag cctatgcctt
    11221 cacgagcaag agcaatagcg tggccgtcgt caccgacggg tccgccgttc tcgggctcgg
    11281 gaacctcggc ccggcagcgg cgcttccggt gatggaaggg aaggtgatgc tctttcggga
    11341 attggccggc atcgacgcct ggccgatttg cgtcagcaca caggatccgg aagaaatcat
    11401 ccgcatcgtc cgcggtatcg ctcccgggtt cggcggaatc aatctggaag atatcagcgc
    11461 cccgcgttgt ttcgagatcg aacgcgctct caagacctcg ctcgacattc ccgtcatgca
    11521 cgacgaccag cacggcacag ccgttgtctt gctggcagcg ttgaccaatg cgctgaaggt
    11581 tgtcggcaag cggatggagg acgttcgcat cgtggtgaat ggcctcggcg cagcaggaac
    11641 ggcctgttgc cgcattctgt tggccgccgg agcctgtcac cttgtcggat gcgacaagga
    11701 aggcatcgtg ttgatggggg aggcagagga actccgcgcc tgccggaccg accttcaggc
    11761 ctgcatccgg cgggaccagc cgcgcggcac cattcacgac gccttgcgcg gcgccgatgt
    11821 cttcatcggg ctctcggtgg ggaatgtgct ggagaaagcc gatctcgaca acatgaactc
    11881 agatcgcatc gtgttcgcca tggccaatcc cgatcccgaa atcgatccga agatcggcga
    11941 tgaagtgtcg cgcatttttg ctaccggccg atccgactat cccaatcaaa tcaataatgc
    12001 cctctcgttt ccgggaattt ttcgcggtgc cctcgacgtg caagccagcg agatcaacga
    12061 agccatgaaa ttagccgcgg cacaggccat tgccgaatgt gtgccgaccg aggccctgtc
    12121 ggaagactat attatcccca gcgtcttcga tcgcgacgtc gtgccgcggg tggccaaggc
    12181 cgtggcggcc gcggctcgca ccaccggtgt cgcccgacgc cgcatcaaaa tcgaagacga
    12241 cccccgctga cccaggccgc tcaccgttcc gatctccgct tgcgcacagt gatcaatcgt
    12301 ttttctgcaa cattggcgtg gtacgcagaa tccatgtaga gtagggccga cgctcgaggc
    12361 cgtcgcccgt ctctcgtggc tttgcctccg cctcagtgag atcatccgga atccagtgtg
    12421 acgccaacca ccattcaact gatcggaacc ctgttgttcg ccgtcgcgat cctgcacacg
    12481 tttgcgacgg cattcttcga acatctggcg cataccagac cggcacatgc cggcctctgg
    12541 catttgctcg gtgaagtcga ggttgcgttc ggattctggg ctctcgtgct catcgtcgcc
    12601 atgtttgtca ccgacggtgc ggctgccgcc acgcactatc tcgactcccg cagttttacg
    12661 gaacccctgt tcgtcttcgc catcatggtg atcgcaggaa ccaggccgat cttacagacc
    12721 gccatgacgg gagtccgtat tgtgagcagt gccatcccgc tgccacaggg aatgcgggtg
    12781 tatctcaccg tgctggcact tgttccgcta ttgggatcct tcatcacaga accggccgcg
    12841 atgacgttgg cggcactcct gctgcgtgat gtcttttttc gccatggctg ctccacgaac
    12901 ctgaaatacg cgactctcgg cgtcctcttc gtgaatgtgt ccatcggggg aacgctcact
    12961 cccttcgctg cgccgcctgt gttaatggtg gccggtgcct ggcagtggga tatcgcattc
    13021 atgctgggca cattcggttg gaaagcggcg ttggctgtat tgatcaatgc gtttggggca
    13081 accatgctgt ttcgtcgaga actcagccgc atctcgaccg ccatcgatca acgcgaggag
    13141 aaccgcgcgc cactggctct ggtgatcaca catcttgctt tgctcgccat ggtcgtggtg
    13201 ttcgcccacc atcccgcgat gttcctgggc atcctgctgt tgtttctcgg gatcgcgcag
    13261 gcctacgagc agtatcagga tcgtctgatt cttcgcgaag ggttgctcgt ctcttttttc
    13321 ctggcaggcc tggtggtctt aggcggacaa caacaatggt ggctgcagcc gatcctgctc
    13381 ggcatgggag ccgatgcggt gttcgtaggc gccacgattc tgactgcctt cactgacaat
    13441 gccgctctga cctatctcgg gtcgctcgtc gaaggccttt cggatgcatt taagtatgcc
    13501 ctggtggcag gcgccgtcac gggcggtggc ttgaccatta tcgccaatgc gccgaatccg
    13561 gccggactgg ccatcctgcg gacctatttc gacgacgagg cggtacatcc gctccgactg
    13621 ttcctggccg cgctgccgcc cacactgatc gcggcagcca tgttctggtg tctgtagcct
    13681 gcgacgcagc aacgattcac tcaatcgact cttatcgccg gacaaagtga tgtgctaaga
    13741 tgcgatcgac ttgcctctta cctgcaaggt cacgcctaac gaggactcgt ggcattttca
    13801 accaatcgcc tgttc
//
LOCUS       DAOQEW010000101        13649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1286_length_13649_cov_7.285935, whole genome shotgun sequence.
ACCESSION   DAOQEW010000101 DAOQEW010000000
VERSION     DAOQEW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1286_length_13649_cov_7.285935"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /gene="secA"
                     /locus_tag="PLS35_11655"
     CDS             complement(<1..2509)
                     /gene="secA"
                     /locus_tag="PLS35_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNI19368.1"
                     /translation="MLTQVLNIIFGSKNDREIKALRPIVEQINGLESSLTPLSDQALA
                     EKTQEFKKRLQDGETLEDILPEAFAVCREMSRRRLNMRHFDVQLIGGMILNKGRIAEM
                     KTGEGKTLVATLPLYLNALEGKGAHLVTVNDYLAKRDASWMAQLYHALGLTVGIIQHD
                     ASFLYDPTYEAADKRLQHLRPCSRHEAYRAEITYGTNNEYGFDYLRDNLIVSDLSQCV
                     QRPLHFAIVDEVDSILIDEARTPLIISGPTDQTTDLYYRINSIIPQLKPEHDYTIEEK
                     TKTASLTEEGNVRVEKLLGVDNLYDLQHMDLVHHVVKALQAYALYKRDVDYVVKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYKKLAGMT
                     GTADTEAGEFAKIYNLDVNVVPTNRSMIRKDYADVVFRTEKEKFTAIVEEIKDCHERG
                     QPVLVGTISIEKSERLAGYLSRNGIKHNVLNAKFHEKEAEIIAQAGRKGAVTIATNMA
                     GRGTDILLGGNADFLFKRILYQDDTLTDARKQEIFDEIKADCEKDKQAVVAAGGLHIL
                     GTERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFASERVSQMMLKLGMEEG
                     VPIEHGMVTRAIANAQKKVEAHNFEVRKQLLEYDDVMNKQREVIYQHRHAVLAGEHIQ
                     QDIHDMMKDLVNAFVETYCPADQYQEEWDYNGLGEALQGQFALDITQGKGSVADHFKD
                     VGRDAMIEEIQTQVRRAYDHKEQELGSELMRYLEKMLLLQVIDHHWKDHLLGMDHLRD
                     GIGLRGYGQKDPLIEYKREGFDMFSSMMERIKSDVLER"
     gene            2762..3844
                     /locus_tag="PLS35_11660"
     CDS             2762..3844
                     /locus_tag="PLS35_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI19369.1"
                     /translation="MRQRVTGSRHLLTTLPLLCVALFVTSLPESSAASSASPESARRS
                     YERGVVLFREGNADGAIEALKKALAQNPKLAEAYHVLGLIYFQSKRNPDEAIQAYKQS
                     LKLSAPSAEILNDLADVYLAQGRGTDAEQVLRQVLDIAPGNEEAHLDLARLYEERHDR
                     TNAVKMYQSLLRIRPDHAEALYHLASLYDSQGDLKQAREYLSRLTQANPGHANAWYLL
                     GRLAERSNDLNAAAAAFKEAIAVKADLVDAHYNLGFVYRSQGLLVEAEREFLEVIRYR
                     PEYAEAHLNLGMVYTSLNRLDEAEREHERAVALKPTSAEAHYNLGVFYELHRKDMGRA
                     LAQYRRYRDLGGRDERVERIIGVGGP"
     gene            3904..4236
                     /locus_tag="PLS35_11665"
     CDS             3904..4236
                     /locus_tag="PLS35_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19370.1"
                     /translation="MRARLWFFTMRSSRFHSLVWVVVFAVVALLAVTTGSAVAHELHH
                     AAHHDAGTHGSGICAWMCATAGVHVVTPVHATHVLALSGDASFLSDIVHLPQVSALCQ
                     SRAPPLSF"
     gene            4461..4913
                     /locus_tag="PLS35_11670"
     CDS             4461..4913
                     /locus_tag="PLS35_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19371.1"
                     /translation="MQVNQWLFRLGVCGLTGLVLSVSTPMVSAADTKAEKAFTIAVTD
                     AQGVETELKNGIFYWEEKMSETSFVPHELRHLPSKRGTATVNIKFDQIKQIELKPGAD
                     KTAPAMTVTLANGKSGEFVPAVSGSFKGESDFGQVEVPIGSLSKVVFK"
     gene            4928..6112
                     /locus_tag="PLS35_11675"
     CDS             4928..6112
                     /locus_tag="PLS35_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNI19372.1"
                     /translation="MSKGVVPMDNVIVAHSGHSNQSVAGWGASLVFHACLALVALGLL
                     PKMTVMVEKEPFKWDVALVEPPREVARLEGPTPAPRVQPPTPTPVKPRSQPVRAVEPP
                     PQPVQRQVETRVLPQAVQREVQPVVETVRPQEQIQSKQEVVQMQAKPVEPQEVHKTEP
                     VVQAVAPAEMQREVAAVVEQQVMEAAPATAQTYQAQQVVSAPAVVETVPEPVMTASAP
                     VVHTPPAVEAVPATAAPVDPAPAAESVAAAVSEPVAEPPAPVEAVVVPTAPVAAVTEP
                     DPSTLREHQVVAQAAVARPAPKADYGWLADSLKRRVIELRHYPSTARLNGWEGKVVLK
                     VSISNDGQLKSVEVVKSSGHESLDQAAMEAVRRACPLHMKHELTAPMVVLNLPLVYSL
                     NR"
     gene            6173..6715
                     /locus_tag="PLS35_11680"
     CDS             6173..6715
                     /locus_tag="PLS35_11680"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNI19373.1"
                     /translation="MIQSLTRNVIVTALLVSTLVGSALAMGSRPPAAGMPASGFSLTD
                     LQGKAHTLEQYRGKIVLVNFWATWCKPCTSEMPAMQTVYDQLRDKDFVVLAVNELEDE
                     AKVREHIQQYKHTFPVLLDRENRVANQYGVFGLPVSVFIDQQGVVQEYIKGGLLTEQK
                     IQEIVSRIQQAAPIKAAALH"
     gene            6747..7031
                     /locus_tag="PLS35_11685"
     CDS             6747..7031
                     /locus_tag="PLS35_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19374.1"
                     /translation="MPLLLVCAFLALQGIAYPSMLEHVAQHAHHEAGVHGTVMCSWMC
                     AAGQDLEPVPEVAPVTISVIALLDEIVVDSVSIESRTLWPSRGPPSSSPA"
     gene            7150..8187
                     /locus_tag="PLS35_11690"
     CDS             7150..8187
                     /locus_tag="PLS35_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19375.1"
                     /translation="MWAPSRRFLVSVLGIVAVAVVSVTSIPQAYAACGAVSCFVTIGS
                     QQQVPQSGLLTMNFIYSYTPMSLPSGSTGLIAEADQIGRRIIPNHHNERSTYTNSFAL
                     DFNYGVTDRLGLQVTLPYLTRRHEHYHFHQGQNEDLSRFSDKGLGDIRVMAKYNVLAS
                     LKNMVVFGVGTELPTGDYRQTDNLGGVLEPAGQLGRGQFGLVGTMYQSYEILPHRLNQ
                     FVFASYRHTFKNNDGYQFGDEYIVNGGFNLVTLPWLVFTAQVNYRYMTHDSLSASIGS
                     GPILDRGIPNTGSSWFGAAPGVLFNIGDTWQAYFYAQVPLYRDANNNLAQATSYTFGV
                     TKYFQLFTPGA"
     gene            8276..9550
                     /locus_tag="PLS35_11695"
     CDS             8276..9550
                     /locus_tag="PLS35_11695"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNI19376.1"
                     /translation="MPRRAKQALCAGLLFGLLPTMPTALAESPAGMQFGPKVTTEHKV
                     KSLVGPSVQIDEAGALSLAWMEENKEVRSVMYARSLEPGGPMGAPVRINRVEDIPYWR
                     QEAPALVVQGEDVFVTWGLAHPKATPEQPFATELRLSHSKDGGRTFQPSIVVNDDPGV
                     IQHTFDALHRDSEGRLHVSWIDGREGKKDPGTYVARSLDQGQTITKNAKVDEGTCVCC
                     RTAITSGPDGTVYVAWRKIFEGNVRETVVARSTDHGETFGSSVIVGHDKWVFPACPHR
                     PASMGVDWQGRLYIVWYTEGTDEIPAVYLAFSDDRGQTFSPKQQLNVSKNTFPDHPQI
                     AVDPEGRLVVIWEEQGPVKRDVVMSASTDRGATFTAPQKLNERKSQTPVVAVNRHGLF
                     AMAWMEHGMPGHKIVMQTLRMPSVTAAAAAGQ"
     gene            9568..10098
                     /locus_tag="PLS35_11700"
     CDS             9568..10098
                     /locus_tag="PLS35_11700"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNI19377.1"
                     /translation="MRLGFVQTFALFVAIGLWCDPALADDRLAALKVGRVAPGTVAAP
                     FELTSLDGAAVDLAQLKGKVVVVNFWATWCGPCKEEMPAFERLRRQLDPERFALMTIT
                     TDLQREGIKQFLANLHVQVSVLFDEDQAVSQAYLVRALPTTVVIDPQGMLIGRAVGPR
                     DWDAPNVVHALQSLMP"
     gene            10095..10415
                     /locus_tag="PLS35_11705"
     CDS             10095..10415
                     /locus_tag="PLS35_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19378.1"
                     /translation="MMRVTKPSIGLAAALAGLYLVLAVFSVACVVDHADPKPAAHHHG
                     GTVSHSSFCAWACQANPTSDAGPVALFLQPFFVATLVVESSHAIVAGGVGFHTASRAP
                     PVQS"
     gene            10642..12867
                     /locus_tag="PLS35_11710"
     CDS             10642..12867
                     /locus_tag="PLS35_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI19379.1"
                     /translation="MEKSARERDLRDQLQNILRELEELQQQPAGGAAAKPIQPAQPAA
                     VEESSASVAAEPIPQYELSDVSIVSDRVQRRPEGLSMSSTEQSETEAQPTRTMKESME
                     SLPGVVLRQANGPRDFSMMIRGQGAKTTFAVRDIKMYEDGFIQTQSDGLSRLDIHDPW
                     FMRSVEVTRGASSSLYDNYALGGMVHFRTRRGSDINGVETFLSGGSFGYHKEGIAVGQ
                     QFENLDASLFVSNVGEDGFIRNSSYNTQTINLNLRFKIDDKQNFYFKAITNWLDARVP
                     TRLTQSQFNADHRQAGGTNVVCNANTCSDAERLAQRRLDRRTIIGGIYERQINANTVL
                     TIEADYDVKDINQTFSQISDNVNPNYKHYADLRHDGRLFDMPLRSYVGFFLSNMEQEA
                     QTFQNLADGFGTRGALLQNSRGTIRNVGGRFREELEFIPKVTLAAGLGFEQSQLSVQS
                     IGYTGGAVSSRAGADRSYTNWAPEMSVTWKPTDAQRHWVRASTGYGIPGFANLLRDPV
                     TGQPGTNFDLKPQKNLNFEIGTESKLTKTLTVQLVGFWTFFKDEIITQAVSGTNTASV
                     NADSSQYKGIEAFYDWRPMEGLRLSGAYTHIEAKYVNFADRLNTAAGFLTRDGNKVPN
                     VPTDVLNTKVAYDHTATGWGAWVEGSYYNSYFLNNSNTFGIPSYMIANVNVHKNFDLK
                     NSWFRFAKFYVEVDNIADKKYAASGQVIGGETAGAAAGQQAFFAGYGRAIYGGVTFGL
                     F"
     gene            12933..13127
                     /locus_tag="PLS35_11715"
     CDS             12933..13127
                     /locus_tag="PLS35_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI19380.1"
                     /translation="MNKQLLRVGEAADVLAVSRWTIYRWVEDGRLEGTKIGRGSLRVF
                     RASLERLVQRNKTHEMNLIQ"
     gene            13199..>13649
                     /locus_tag="PLS35_11720"
     CDS             13199..>13649
                     /locus_tag="PLS35_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19381.1"
                     /translation="MLCLFALALVAHASGCAGGAETQAVLPAKPYAFVHGQWFDGEGF
                     QSDTWYAVQGRLTRQVPSGPVETVDLSGLYMVPPFGEAHNHNVEGPWNVQAVAKRYLQ
                     DGVFYVKNPNDVRDFALQIRQAINVPTSIDATFAHAGLTGRGGHPVAL"
BASE COUNT         2686 a         3807 c         4228 g         2928 t
ORIGIN      
        1 tgcgctccag cacatcggac ttgatgcgct ccatcatgga ggagaacatg tcgaaccctt
       61 cgcgcttgta ctcgatcaac ggatctttct ggccataccc gcgcaggccg atgccatccc
      121 gcaaatggtc catgcccagc aggtgatcct tccagtgatg gtcgatcact tggagcagga
      181 gcattttttc gagataccgc atgagttcag agcccagttc ctgttctttg tggtcatagg
      241 ctcgccgcac ctgggtctga atctcctcga tcatcgcatc ccggccgacg tccttaaagt
      301 gatcggcgac actgcccttg ccctgggtga tgtcgagggc gaactggccc tgcagcgctt
      361 ctccgagacc gttgtagtcc cactcttcct ggtattgatc ggcgggacag taggtctcga
      421 cgaaggcatt caccagatcc ttcatcatgt cgtgaatgtc ctgctggatg tgttcccccg
      481 ccaggacggc atgccggtgc tggtagatca cttcccgctg cttgttcatg acatcgtcgt
      541 actcaaggag ctgcttgcgg acttcgaagt tgtgcgcctc gacttttttc tgcgcgttcg
      601 cgatggcgcg ggtgaccatg ccatgttcga tcgggacacc ttcctccatc ccgagcttga
      661 gcatcatctg cgagacgcgt tcggacgcga agatccgcat gagatcgtct tcgagcgaca
      721 ggtagaagcg tgaagatccg ggatcgcctt ggcggccggc ccgaccacga agctgattgt
      781 cgatgcgccg gctctcgtgc cgctcggtgc cgaggatatg gagacctccg gccgcgacca
      841 cggcctgctt gtccttctcg caatcggctt tgatttcgtc gaaaatttcc tgctttcgcg
      901 cgtctgtcag cgtgtcgtct tgatagagaa tgcgcttgaa gaggaagtca gcattaccgc
      961 cgaggagaat gtccgttccg cggccggcca tattggtggc gatggtcacg gcgcctttgc
     1021 gaccggcctg ggcgatgatt tccgcttctt tctcgtgaaa cttggcgttc aacacgttgt
     1081 gcttgatgcc attgcggctc aagtagcccg ccagacgctc agatttctcg atggaaatgg
     1141 tgccgacgag gactggttgt ccgcgctcgt ggcagtcttt gatttcttcg acgatggctg
     1201 tgaacttttc tttttcggtg cggaagacga cgtcggcgta gtctttgcgg atcatcgacc
     1261 ggttggtcgg gaccacgttg acgtccagat tgtagatctt ggcaaactcg cccgcttccg
     1321 tatcggcggt gccggtcatg ccggccagtt ttttgtacat ccggaaataa ttctggaagg
     1381 tgaccgaggc cagggtttgg ttctcgttgg cgatcttgac gccttctttg gcttccacgg
     1441 cctggtgcag cccgtcgctc caccggcggc cgggcatcaa gcgtccggta aattcgtcga
     1501 cgatgatgac ttcgccatcc ttgaccacat agtccacgtc acgcttgtag agggcatagg
     1561 cctggagggc cttgacgacg tggtgtacca gatccatgtg ctgcaggtcg tacaggttgt
     1621 ccacgcctag cagtttctcc acgcggacgt tgccctcttc cgtgagcgag gcggtcttgg
     1681 ttttttcttc gatcgtgtag tcgtgctccg gtttgagctg cggaatgatc gaattgatgc
     1741 ggtagtacaa atccgtggtt tgatccgtcg ggccggaaat gatcaagggc gtgcgcgcct
     1801 cgtcgatcag aatgctgtcg acttcgtcca cgatggcgaa gtgcagcggg cgctggacgc
     1861 actggctcaa gtcgctgacg atcaaattgt cgcggaggta atcgaagccg tattcgttat
     1921 tggtgccgta ggtgatctcg gcgcgatagg cttcatgccg gctgcagggg cggaggtgct
     1981 ggaggcgttt gtccgccgcc tcgtaggtcg gatcgtaaag aaaagaggca tcgtgctgga
     2041 tgatgccgac cgtcaggccg agcgcatgat agagctgggc catccaggag gcgtcgcgtt
     2101 ttgccaggta gtcgttgacc gtgaccaggt gggcgccctt cccctccagc gcattgagat
     2161 acagggggag ggtggcgacc agggttttac cttcaccggt cttcatttcg gcgatccggc
     2221 ctttgttcag aatcatgccg ccgatcaact gcacgtcgaa gtggcgcatg ttcaaacggc
     2281 ggcgagacat ttcgcggcag acggcaaacg cctcgggcag aatgtcctca agggtctcac
     2341 cgtcctggag acgcttcttg aattcctgcg ttttctccgc cagggcttga tcggacaggg
     2401 gcgtgaggct tgattccagc ccgttgatct gctcgacaat cgggcgcaat gccttgatct
     2461 cacgatcgtt tttactgccg aatatgatgt tcagaacttg ggtcagcatg cagacgtacc
     2521 tttattctct cgaacctgcc gggagacgaa gctcgcccct ctggcacggg ctcggcggcg
     2581 gagtatacag gaatccgtca tcgaatccta ccgaaacagg ggcgacggtc tcaactgttt
     2641 cacgatgaac gactgcgaca ggtgctcgtc gttgctttcg gatgcggtga catctatagt
     2701 gggcccacca tctcgccggc tcttacggcg aagtcaccca cggattccat tgcggatttc
     2761 gatgcggcaa cgggtcaccg gttcacgtca tcttctgacg actcttccac tcttgtgcgt
     2821 ggccctgttc gtcacctctc tgcctgaatc ttcagccgcc tcaagtgcct ctccggaatc
     2881 ggcacgtcgc agttacgagc gcggcgtggt tctgtttcgt gaaggcaatg ccgacggcgc
     2941 catcgaagcc ctgaagaaag cgctcgcgca aaatcccaaa ctggcggagg cgtaccacgt
     3001 cctgggattg atctattttc agagcaaacg caatcccgat gaagcgattc aagcctacaa
     3061 acaatcgctg aaattgagcg caccgtcggc tgagattctc aacgatctcg ccgatgtcta
     3121 tctggcgcaa gggcgaggga ctgacgcgga gcaggtgctg cggcaggtgt tggatatcgc
     3181 gcccggcaat gaagaagcgc acctggattt agcgcggctc tacgaggaac ggcatgatcg
     3241 cacgaatgcc gtgaaaatgt accaatcgct ccttcgcatc cgaccggatc acgcggaggc
     3301 gctctatcac ctggcgagtc tctatgacag tcaaggcgat ctcaagcaag cccgcgaata
     3361 cttgtcccgc ctcacgcagg ccaatcccgg acatgccaac gcctggtatc tcctggggcg
     3421 tctggccgaa cggagcaatg acctgaatgc tgccgccgcc gcgttcaagg aagccattgc
     3481 ggtcaaggcc gatctggtgg atgcccatta caatcttgga ttcgtctatc ggagccaggg
     3541 attgctggtc gaagccgagc gagagttttt ggaggtgatc cggtatcgcc ccgagtatgc
     3601 cgaagcccac ttgaacctgg ggatggtcta taccagcttg aaccggctgg atgaggctga
     3661 gcgggagcat gaacgggccg tggcgctcaa gcccacctcg gcggaagccc actacaatct
     3721 cggggtgttt tacgaactcc acaggaaaga catggggcgt gccctggcgc agtaccgccg
     3781 gtatcgtgac cttggcggac gtgatgagcg cgttgagcgg atcattggtg tcggcgggcc
     3841 ctagtcgtca accccgcaca gggtgaaaag ttgcgttgac gctccctcgg cgagtttgct
     3901 agcatgcgcg ccaggttgtg gttctttact atgcgaagtt cccgctttca tagcctcgtc
     3961 tgggttgtcg tctttgccgt cgtagccctc ctggcggtca cgaccggcag cgcggtcgcc
     4021 catgagctgc accacgccgc gcaccacgat gccggaacgc acgggtcagg catctgtgcc
     4081 tggatgtgcg ccacggccgg tgtacatgtg gtcacgccgg ttcatgcgac gcatgtcttg
     4141 gcgctctccg gcgatgcctc gttcctttcc gatatcgtcc atctccctca ggtctctgcc
     4201 ctctgtcagt cgcgcgctcc acccctgtcg ttctaattcc acgtctccct tgtgcatagt
     4261 ctcgctccgc gactggtcgt cggggagccc ggatcaaaca gttgagcgag gtctctcgca
     4321 tgctgtggat gcattcctcc tttcgatggt cgcttcgcga tggtgtctgt tccgtcgctt
     4381 cgcctggagg ctggatcccg tgaatcgtta ggaatggtgt ggcgtcaatt cctctgcgaa
     4441 gacgtgaagg agtgatgaag atgcaggtga atcagtggtt gttccgtctg ggcgtatgcg
     4501 gtcttacagg gctggtgttg tcggtgtcga ctcccatggt ctccgctgcc gacacgaagg
     4561 ccgaaaaggc attcaccatt gcggtgaccg atgctcaggg tgtcgagact gagctgaaga
     4621 acggcatttt ctattgggaa gaaaaaatga gtgagacgtc gttcgtcccg catgagctgc
     4681 ggcacctgcc atccaagcgc ggcacggcga cggtcaatat caagttcgat cagatcaaac
     4741 agatcgagtt gaagccgggt gccgacaaga ctgcaccggc tatgaccgtg accttggcca
     4801 acgggaagag cggggaattt gttcccgcgg tgagcggcag tttcaagggc gagtccgatt
     4861 tcggccaggt tgaagtcccg atcggttcgc tctctaaagt ggtcttcaag taacgtcgga
     4921 acgtatcgtg tcgaaagggg tcgttcccat ggacaatgtc atcgtcgcgc actcggggca
     4981 ctcaaaccaa tcggtcgcag gatggggggc gtcgctggtc tttcacgcct gccttgcgct
     5041 ggtcgcgttg ggtctcctgc cgaagatgac cgtcatggtg gagaaagagc cgttcaagtg
     5101 ggacgtggcc cttgtcgagc cgccgcgtga agtggcgcgc ctggaaggcc caactcctgc
     5161 gccccgggtc cagccgccca ccccgacccc ggtgaagccg cgatcccagc cggttcgtgc
     5221 ggtcgagcca ccacctcagc cggtgcaacg gcaggtggaa accagagtgc tcccgcaagc
     5281 cgtgcagcgt gaagtccagc ccgtggttga aacggtgcgt ccgcaggaac agatccagtc
     5341 gaagcaggaa gtcgtgcaga tgcaagcgaa gccggttgag ccgcaggaag tccacaagac
     5401 cgaaccggtg gtgcaagcgg tggctccggc cgaaatgcag cgtgaagtgg cggcggtagt
     5461 ggagcagcag gtcatggagg cggcgcctgc cacggcccag acctatcagg cgcagcaggt
     5521 tgtcagcgca ccagcggtgg tcgaaacggt ccctgagccg gtgatgacgg ccagcgcacc
     5581 ggtggtgcac acccctcccg cagtcgaagc ggtgccggcg accgcggccc cggtcgatcc
     5641 cgcccctgcc gcggaatcgg ttgcagcagc tgtcagtgaa ccggtcgccg agcctcctgc
     5701 tcccgttgag gcggtcgtgg tgccgactgc gccggtggca gcggtgactg agccggaccc
     5761 ctcgacgttg cgtgaacatc aagtggtggc ccaggcagcc gttgcgcgac ctgctcccaa
     5821 ggccgattac ggctggctcg ccgactcttt gaaacggcgc gtcatcgaac tgcgacatta
     5881 tcccagcact gcgcgcttaa acggctggga ggggaaggtc gtgttgaagg tgtctatcag
     5941 caatgacggg cagctgaaaa gtgtcgaagt ggtcaagagt tcaggacatg aatcgctgga
     6001 ccaagcggcc atggaagcgg tgcgacgggc ctgcccattg cacatgaagc atgagctcac
     6061 cgcgccgatg gtcgtgctga atctgcccct cgtatacagt ttgaaccggt aaggtgattg
     6121 gggagaaggc aacatgaaca ctcaatcagg agcctgtcgg agatcggcaa tcatgataca
     6181 gagccttacg cggaatgtaa tagtgacggc cctgctcgtc tcgacgttag tcggctcggc
     6241 actggcgatg gggtcgaggc ctccagcggc ggggatgccg gccagcgggt tttcgctgac
     6301 ggatttacag ggcaaggcgc acaccctgga gcagtataga ggaaagatcg tcttggtgaa
     6361 tttttgggcc acctggtgca agccctgcac gtccgaaatg ccggcgatgc agacggtcta
     6421 tgaccagctt cgtgataagg atttcgtggt gctggcggtc aatgaattag aggatgaagc
     6481 caaggtgcgt gaacacattc agcagtacaa acatacgttt cccgtgttgc tcgatcgtga
     6541 aaaccgggtg gccaatcagt acggggtgtt tggtctgcct gtgagcgtgt ttatcgacca
     6601 gcagggcgtg gtgcaggaat acatcaaggg cgggttgttg accgagcaaa agatccagga
     6661 gatcgtcagt cgaattcaac aggctgcgcc cattaaggcg gcggcgttgc attgacccaa
     6721 tgaggactcg cgtacgacat accctgatgc cgctcctgct ggtctgcgcc tttctggcgc
     6781 tgcagggcat tgcgtatccc tccatgctgg agcatgtggc gcaacatgcg catcatgagg
     6841 ccggtgtcca tgggaccgtg atgtgttcct ggatgtgtgc agcggggcaa gatctcgagc
     6901 ccgtgcctga ggtggccccc gtcacgatct ctgtcattgc cttgcttgat gagatcgtcg
     6961 tcgattccgt ctcgatcgaa tcccgcaccc tgtggccgtc gcgaggcccg ccgtcttctt
     7021 ctccggcctg aatcgtttcc ctctgattca tcccgtttcg gtatcacccg tagcgtctga
     7081 gccggggttc catccggcac caggcgcgag ctgtatcatc aaccgtgatt ggagaaagga
     7141 tcgtgtgtca tgtgggctcc cagtagaagg tttttggtgt cggtactcgg aatcgtcgcg
     7201 gtggctgtgg tgagtgtgac gtccatacct caggcctatg ccgcctgcgg tgccgtcagt
     7261 tgcttcgtga caattggatc tcagcagcag gtcccgcaat ccggcctgct gaccatgaat
     7321 tttatctaca gttatacgcc aatgagcctg cccagcggat cgacgggcct gattgccgaa
     7381 gcggaccaga tcgggcggcg catcattccc aaccatcata atgaacgctc aacctatacg
     7441 aactccttcg cgctggattt taattacggc gtgaccgatc gcctgggatt gcaggtcacg
     7501 ttgccctatc tgacccgccg gcatgagcac taccatttcc atcagggaca aaatgaagat
     7561 ctgagccgct tttcggacaa ggggcttggc gatattcgag tgatggcgaa atacaatgtt
     7621 ctcgcttcat tgaaaaatat ggtggtcttc ggggttggta cggagctgcc gaccggcgat
     7681 taccggcaga ccgacaatct gggcggagtc ttggaaccgg caggacaatt ggggcgcgga
     7741 caattcgggc tcgtgggcac gatgtaccaa tcgtatgaga tcctaccgca tcggttgaat
     7801 caattcgtgt tcgccagcta tcgacatact ttcaagaaca acgatgggta tcagttcggc
     7861 gacgaataca ttgtcaatgg cgggttcaat ttggtgacgt tgccatggct ggtattcacg
     7921 gcccaggtga actaccgcta tatgacgcac gacagtctga gcgcgagcat cggttccggg
     7981 ccgatcctgg accgcgggat ccccaacacc ggctcttcct ggttcggtgc tgcgccgggc
     8041 gtgctgttca atatcgggga cacatggcag gcctattttt atgcccaggt gcctctctat
     8101 cgggatgcca acaacaacct tgcgcaggcc accagttaca catttggcgt gaccaagtat
     8161 tttcaattgt tcacgcctgg agcgtgagga gtttgacgca gtgtacggtc tagcttcagt
     8221 agccgacggg ggtaaaacaa tgctccaaag tatgcgcggt ctacggacga gtctcatgcc
     8281 gagacgagca aaacaagcct tgtgtgccgg gctgttattc ggattgctac ccacgatgcc
     8341 cacggcattg gcggagtctc cagcgggtat gcagtttggt cccaaggtca ccaccgaaca
     8401 taaggtgaaa agcctcgtgg gcccgtcggt gcagatcgac gaggcggggg ccttgtcgct
     8461 ggcctggatg gaagaaaaca aagaggtgcg gtctgtcatg tatgcccgga gtctcgagcc
     8521 tggaggaccg atgggtgccc cagtgcgcat caaccgcgtg gaggacatcc cgtactggag
     8581 acaggaagcg ccggcgctcg tggtgcaggg ggaagacgtg ttcgtgacct ggggcctggc
     8641 ccatcccaag gctacgcccg agcaaccgtt cgcgaccgaa ttgcgactga gccattcgaa
     8701 ggacggcggc cggactttcc agccgtcgat cgtagtgaat gacgatccgg gtgtcatcca
     8761 acataccttc gatgcgctcc atcgcgattc ggagggacgg ttgcatgtgt cctggatcga
     8821 tggacgtgaa ggcaaaaaag atcccggcac ctatgtcgcg cggtcgctgg atcaggggca
     8881 gacgatcacg aaaaatgcca aggtggacga ggggacctgt gtctgttgcc gaaccgccat
     8941 cacgagcgga cctgacggaa cggtgtatgt ggcctggcgg aaaatttttg aggggaacgt
     9001 gcgagaaacc gtcgtggcgc gttcgacaga ccatggtgag acgttcggct catcggtcat
     9061 cgtcggacat gacaaatggg tctttccagc ctgtccgcat cggcccgcct cgatgggcgt
     9121 cgactggcag ggacgactgt atatcgtctg gtatacggaa gggaccgacg aaattccggc
     9181 ggtctatctc gccttctccg atgatcgagg ccagacgttt tcgccgaagc agcagttgaa
     9241 tgtgtcgaag aacacctttc ccgaccatcc gcagatcgcg gtggatcctg aaggcaggct
     9301 ggttgtgatc tgggaggagc agggaccggt caaacgagat gtggtcatga gtgcctccac
     9361 cgatcgcggg gcaacgttca ctgcgccgca gaagctgaac gagcgaaaaa gccagacacc
     9421 ggtagtcgcg gtgaatcggc acgggttatt tgccatggcc tggatggaac atgggatgcc
     9481 gggccacaaa attgtcatgc agaccctgcg gatgccgtcc gtcacggcgg cagcagcggc
     9541 aggccagtag atgggacggc gcgtgtgatg cggttgggat tcgtgcagac ctttgccctg
     9601 tttgtggcga tcggactctg gtgcgaccct gcactagccg acgaccggct agcggcgctg
     9661 aaggtcggac gggttgcgcc cgggactgtg gcggccccgt tcgagctcac atcactcgat
     9721 ggcgcagctg tcgacctcgc gcagttgaag ggaaaggtcg tcgtggtgaa cttctgggcc
     9781 acctggtgcg ggccctgcaa ggaagagatg ccggccttcg agcgattgcg ccggcagctg
     9841 gatcccgaac gttttgcgct gatgacgatc acgaccgacc tccaacgcga gggcattaaa
     9901 cagtttctgg ccaatcttca tgtgcaggtg tccgtgttat tcgatgaaga tcaagcggtg
     9961 tcccaagcct acctcgttcg ggccttgccg acgacggtag ttatcgaccc acagggcatg
    10021 ttgatcggcc gtgccgtcgg accccgcgac tgggatgcgc ctaatgtcgt gcacgcgttg
    10081 cagagcctga tgccatgatg cgtgtgacga aaccttccat tggacttgcg gccgcgctgg
    10141 ctggactcta cctggttctc gccgtcttct cagtcgcctg cgtagtcgat catgccgatc
    10201 cgaaaccggc cgcccatcac cacggcggta cggtctctca ttccagcttc tgtgcctggg
    10261 cctgtcaggc gaatccaaca tccgatgccg gccctgtggc gctctttctg cagccgtttt
    10321 tcgtggcgac gcttgttgtc gagagcagcc atgccatcgt cgcgggaggc gtgggcttcc
    10381 acaccgcctc gcgcgcgcca ccagttcagt cataactctt ttttatagcg ctaggtcgtc
    10441 tagcttctct tctcgtccgt tcagacaggg gcgcgagggg attgtctctt ctgtcgtgtg
    10501 cacagttgtg ccgggaggat gtgtatgtgg gcccgccgtg attcattcag acttcatgcc
    10561 gctttatgtt gtgccttgtt gtcagtgcca gtgaccgttg tgccctcaat agcgcaggaa
    10621 gagacagcgg ctggcgccgt tgtggagaaa agcgcacgcg agcgcgacct gcgtgaccaa
    10681 ctgcagaaca ttcttcgtga gttggaagag ttgcagcagc aaccggccgg cggcgcagcg
    10741 gccaagccga ttcaaccggc ccagccggcg gcagttgagg aatcgtccgc atcggtcgcg
    10801 gccgagccga taccgcaata cgagctgtcc gacgtcagta tcgtgagcga tcgtgtgcag
    10861 cgaaggcctg agggtttgtc gatgtcgtcg accgaacaat cggaaacgga agcccagcca
    10921 acccgaacga tgaaggaatc gatggagtcc ctgcctggcg tggtattgcg gcaggccaac
    10981 ggtccgcgcg atttcagcat gatgatccga gggcaagggg ccaagacgac gttcgccgtc
    11041 cgtgacatca agatgtacga ggatggattc attcaaaccc aatctgacgg cttgtcgcga
    11101 ttggacattc atgacccctg gttcatgcgg agcgtggaag tgacgcgggg tgcctcgtca
    11161 tcgctgtatg acaactatgc cctgggtggg atggtgcact ttagaaccag gcgggggagc
    11221 gatatcaacg gagtcgagac attcctgagc ggcggctcct tcggctacca caaggaaggc
    11281 attgccgttg gccagcaatt cgaaaatctc gatgcgtcgc tgttcgtcag taatgtgggg
    11341 gaagacggat tcatccggaa cagcagttac aacactcaaa cgatcaacct gaacttgcgg
    11401 ttcaagatcg acgacaagca gaacttctac ttcaaggcca tcacgaactg gctcgatgcg
    11461 cgagtgccga cccgtttgac tcaatctcaa ttcaacgccg accatcggca ggccggtggt
    11521 accaacgtgg tgtgtaatgc caacacctgt agcgatgcgg aacggctggc gcagcgccgg
    11581 ctcgatcgac gtaccatcat cggaggtatc tacgagcgtc agatcaacgc caatacggtg
    11641 ctgacgatcg aggctgatta cgatgtgaag gacatcaatc aaaccttctc tcaaatcagc
    11701 gacaacgtga atccgaacta taagcattac gccgacctgc gccacgacgg acgactgttc
    11761 gacatgccgt tacggagtta cgtcggcttc tttctcagca acatggagca ggaagcgcag
    11821 acgtttcaga acctcgccga cggcttcggc actcgcggcg cactcctcca aaattcacgc
    11881 ggcacgatca gaaacgtcgg tggccgtttc cgggaagaac tggaatttat tcccaaggtg
    11941 acactcgcgg ccggattggg atttgagcag tctcagctga gcgtccagtc gatcgggtac
    12001 acgggcggtg ccgtaagcag ccgtgcgggc gctgatcgca gttacaccaa ttgggcgccg
    12061 gaaatgtcgg ttacgtggaa gccgactgat gctcaacggc attgggtgcg cgcatcgacc
    12121 gggtatggca ttccgggatt tgccaatctc cttagagatc cggtgaccgg gcagccggga
    12181 acgaacttcg acctgaagcc gcagaagaat ctcaacttcg aaatcgggac cgagtccaaa
    12241 ctgacgaaga ccctcaccgt gcaactcgtc gggttctgga ccttcttcaa ggacgaaatc
    12301 atcacacagg cggtgtcggg taccaacacc gcctcagtca atgctgactc ctcacagtac
    12361 aagggcatcg aagcgttcta cgactggcga ccgatggagg gtttgcgatt gtccggtgcg
    12421 tatacccata tcgaggcgaa gtacgtcaac tttgccgatc ggttgaacac cgccgccggc
    12481 ttcctgacgc gcgacggcaa caaagtgccg aacgtgccga ccgatgtgct gaatacgaag
    12541 gtggcttatg accatacggc cactggctgg ggcgcctggg tcgaggggag ttattacaac
    12601 agttacttcc tgaacaacag caatacgttc ggcattccgt cctacatgat cgcaaacgtc
    12661 aatgtgcaca agaacttcga tctcaagaat tcctggttcc gattcgcgaa gttctatgtg
    12721 gaggtagaca acatcgccga caagaaatac gcggcttccg ggcaggtgat cggcggagaa
    12781 accgccgggg ctgccgccgg gcaacaggcg ttctttgccg gctacggccg cgcgatttat
    12841 gggggcgtga cgttcggttt gttctaaccc tgtggtcgtg agcggcaacg tcgatggttg
    12901 ccgctcacat cactacttca aaggagattg cgatgaacaa gcaactcttg cgtgtgggag
    12961 aagcagcgga tgtgttggcg gtgagccggt ggacgattta tcgctgggtg gaggatggac
    13021 ggttggaagg caccaagatc ggacgtggca gtctgcgggt gtttcgtgcc tcgctggaac
    13081 gcctggtcca acgcaataag acgcacgaaa tgaacctcat ccaatgacga acaggaacag
    13141 ggggaggcga aggggcgtca gggccggtgc gtccatcagt cggcgtggtc tgcgtggttt
    13201 gctgtgtctc ttcgcgttgg cgttggtggc gcatgcatcc ggctgtgcgg ggggtgcgga
    13261 aactcaggct gtgctgcccg cgaaaccgta tgccttcgtc catggccaat ggttcgatgg
    13321 cgaaggcttt cagtcggata cctggtatgc ggttcaggga cgcctgacac gtcaagtgcc
    13381 gtcggggccg gtcgagacgg tggatctctc tggtctctac atggtgccgc cgtttgggga
    13441 ggcgcacaac cacaacgtcg aagggccgtg gaatgtgcag gctgtcgcga agcggtacct
    13501 gcaggacggg gtgttttatg tgaagaatcc caacgacgtg cgggacttcg ccctacagat
    13561 ccggcaagcg atcaacgtgc cgacgagtat tgacgccacc tttgcgcatg ccggcctcac
    13621 gggccgcggc gggcatcccg tggccttgt
//
LOCUS       DAOQEW010000102        13442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1316_length_13442_cov_8.551953, whole genome shotgun sequence.
ACCESSION   DAOQEW010000102 DAOQEW010000000
VERSION     DAOQEW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13442
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_13442_cov_8.551953"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1925)
                     /locus_tag="PLS35_11725"
     CDS             complement(12..1925)
                     /locus_tag="PLS35_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19382.1"
                     /translation="MRAARRTGGLQRKFFVALLIVGIVPGMAALWATYRSSTASLKEA
                     IGEGFQEIARSTSIRLAGAVDDEIDRAGRLAISVANQHAQAQSKADSNKTEAARVHST
                     HRPVNSPVTAAYLEDWAREARHYLRVTVADHRGLVIASTDRALPPQQADQSWWREAMQ
                     APAGTAYVSNVTIDPAINDATFHVAVPIVEKAGGAVTGAVDLVIRHTLLTQLILPIHI
                     GNTGHAMLLDTQGTPLICPVLPPTAHLIPANLMNRLALDHPLWLVAEDDAHGGRDAIV
                     GASPVRFSHPLTRSSLEGNRWYAFVRQAPEETYAPIYSLLLSVGVIGFGLVIALSMFG
                     FVVGYRIVRPLTALQREAEGLRLTLATTQTDHALPLAATPLPREYRTGDEIEDLATTF
                     SAMRAVLEENLRTIRAQQHELIRQEKLASVGQLLAALAHDLRNPLGVIRSSAQVVLEG
                     PQEEGIRHEMARYIIDEVDKLSQRLNDFLRYARQKPPDLADCAAEGIIGAALKQWEAQ
                     GGHEHIRVEQRFSHDLPMIRIDPEQIKDALVNVLINAREAMTEGGGTLTLTTRSAEGG
                     TVEINVTDTGSGIAPDHLGRIFEPFFTTKTYGTGLGLTNVKRLVEDNGGTLIVTSKQG
                     KGTTCTLRFRPAT"
     gene            complement(1925..3310)
                     /locus_tag="PLS35_11730"
     CDS             complement(1925..3310)
                     /locus_tag="PLS35_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI19383.1"
                     /translation="MHAPTLLIVDDEERMRRLFELVLKAAGYQLLLARSGDEALHMLQ
                     EHDSIDMIITDLQLGTVSGMDVLEAAHKQAPDVPVLIVTGYGTVKSAVEAMQKGAYDY
                     ISKPVDNEELKIVIARALQVRQLARDNRTLRAGLQEQFGFDRIVSVSKEMELVKRLAK
                     EVAQTDATVLITGESGTGKDLLARAIHLASPRAQGPMVALNCAGIPEHLLESELFGYE
                     KGAFTDAKKSKPGRFQMADRGTLFLDEIGELSLTAQAKLLRVLEQHVVEPLGGVRSIP
                     VDLRVIAATNQELPDLIKAGRFRLDLYYRLNVYQLRMPPLRERPGDIEPILTQFLDRA
                     RRERGSRIKGIAQNALALLTQYPWPGNVRELHNVVEWLTITCKQDMIQPEHLPASLTA
                     AAPQSSASPAGAPSLLSLGLSVEEVEKAMLQEALAKSGGNVSEASRLLKITRNTLRYR
                     MAKHNLSQPQG"
     gene            complement(3400..4440)
                     /gene="queG"
                     /locus_tag="PLS35_11735"
     CDS             complement(3400..4440)
                     /gene="queG"
                     /locus_tag="PLS35_11735"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNI19384.1"
                     /translation="MRETVMPEPLTALITQAARELGFDVVGISRLPPQPAPNHNPSVS
                     PHRLLDRLHEWLQRGFHASMNWMTRAPERRADPALVLPGCRSIISVGMNYYTDHRADE
                     RPGNGRIARYAWGLDYHNIMGDRLTQLSERLATLAPEDRHRAYVDTGPVMEKAWAQQA
                     GLGWIGKHSNLVSPEFGSWLLLGEILTTLELDADEAGTDLCGSCTLCIQACPTGAITE
                     PYVVDAGRCISYLTIELRGSEPTLSEELRRGIGNRIFGCDDCLDICPYNHQAEPTSEP
                     GFQPTPLTTSPSLRSLMDMNEATFAATFKQSPVRRAKHAGLQRNVSWALANEAAPAGN
                     GTPASSSSRDSR"
     gene            complement(4425..4799)
                     /locus_tag="PLS35_11740"
     CDS             complement(4425..4799)
                     /locus_tag="PLS35_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19385.1"
                     /translation="MSWLVRLISPGLLSVQIALLVMPFDAQAQAPADSAATTEHSCSF
                     GMAKGDPAHQCQVPIPAGCVIATFPGTDKPWTTVSKAGRTFCAFDKKGTDWKNKITGQ
                     CTRCDSSHCTGQFMVRFECAKP"
     gene            5069..5875
                     /locus_tag="PLS35_11745"
     CDS             5069..5875
                     /locus_tag="PLS35_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19386.1"
                     /translation="MTGNNPVFKPFSDCTMAQEKILIVDDEVVVAEDIRRQLRSLGYV
                     VVGVVASGSEAVDMAGEHRPDLILMDIKLKGPLDGIDAARMIQARYGTPVIYLTAFSD
                     EDTLKRARYTLPLAYLIKPFVSSDLRAAIELALFRHRVLRIAEQRGRWLDSVLQSMSE
                     AVVTIDAQGKVTLLNQAAEGLTGWSQPDALGKPIQDVMVVVDAERRTPLVNPALSVLQ
                     QIKAGDRGTRPFLLLSRQGVEHVIHESATVIHDEQGGLTGAVLVFRLAIE"
     gene            6151..6744
                     /locus_tag="PLS35_11750"
     CDS             6151..6744
                     /locus_tag="PLS35_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNI19387.1"
                     /translation="MVQLMSFLGTLLGCLMAFVLATTPQSWAGEVGSIHLLRPLLVQS
                     EPGGGVKATATLLFPGSPSVLHSILTEYRKWPELFETRMRMAQVEEREGHVLTDVYIS
                     HALLPGEQRLLCESQVLPGGGLVTDLKGGDFKQYHREWRLQAAGEGTQTRAEFELLVE
                     PKTMIPDWLIAVAMERELTVHFRLLRQRALDLVTKGR"
     gene            complement(6728..7906)
                     /gene="thiI"
                     /locus_tag="PLS35_11755"
     CDS             complement(6728..7906)
                     /gene="thiI"
                     /locus_tag="PLS35_11755"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HNI19388.1"
                     /translation="MHCAIIHYHELALKGRNRDFFEQRLVRNIRLALKDLKIRRIESL
                     QGRIRVTIPEDVTPDLVTERLRRICGVSNFLLSESVPLDIREPDLSSIKQVAQRQLGT
                     QTFTSFRVTAKRADKRLPLTSMDVEREVGAYLHEAMGKAVKLKQPDVTLYIELLTKEA
                     HVAARKIEGPGGMPVGTSGKVACLISGGIDSPVAAYRMIKRGCKAVFVHFSGRPLVSR
                     ASEEKVQELVQLLTTYQYHSKLYIVPFGEIQQEIVANAPAAFRVVLYRRIMVQIAQEL
                     ARREGCWALVTGDSLGQVASQTAENLVVVENAAEIPLLRPLIGMDKIEITEQAQQIGS
                     FTTSIEPDQDCCKLFVPLHPSTRTKLEDIVRIERGLEISAFVKRGVEKAELTTFTFPS
                     "
     gene            complement(8210..9136)
                     /gene="trxB"
                     /locus_tag="PLS35_11760"
     CDS             complement(8210..9136)
                     /gene="trxB"
                     /locus_tag="PLS35_11760"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNI19389.1"
                     /translation="MHHLVIIGSGPAGLTAAIYAARANLSPLLIEGWQSGGQLTTTTE
                     VENYPGFSKGIMGPELMKEMRGQAERFGTEFLTGDVSAIDLSTRPFRLTIDGEQTIGA
                     SALILATGASPIRLGLPNEHRLTGYGVSTCATCDGYFFRGQDLVVVGGGDSAVEEATF
                     LTKFARTVTLVHRRDKLRASKIMQDRAMNNEKISFAWNSIVEDILGKDLVTGVRLKNL
                     VTGHSTDLPCAGVFVAIGHRPNTDLVKGQLEMDEQGYILTSRGTNTSVPGVFAAGDVQ
                     DAKYRQAITAAGSGCMAAIDAERFLESALHSL"
     gene            complement(9246..9866)
                     /gene="coaE"
                     /locus_tag="PLS35_11765"
     CDS             complement(9246..9866)
                     /gene="coaE"
                     /locus_tag="PLS35_11765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247713.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNI19390.1"
                     /translation="MVSSRPMIFVGLTGGVATGKSTVAKMLERCGALVIDADALAHQV
                     MEPGKPAWRAIVKTFGPAVINPDRTINRPALGTMVFRNRARLRRLEQIIHPRVAREQA
                     RLTRLAVRKDPNAVVIYDVPLLYEAGIDARVDKVIVVTADRETQIARLKKRNGLTRSE
                     ALRRIRNQWPLRKKVAAADFVLDGTTARPRLFTLVKSLYRELARLA"
     gene            9932..10963
                     /locus_tag="PLS35_11770"
     CDS             9932..10963
                     /locus_tag="PLS35_11770"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HNI19391.1"
                     /translation="MKIMNYWILQIAFLCVSVGTVSMVSVKAEEPPAERQPLVVALDG
                     SGQYRSIQEAVDAAKKGDTILIRPGAYPEDVTIHSKENVRLIGAGMDQVTILGRERVG
                     VFHIGKWPYGATNIEISGITVNEHGGHAMGMFNGRGIHFHHARVKGMLFTQQVEDVRI
                     ENCDIGGSETTGVQFANSQAVMRGNFIHDNDHGVSVAGKSTVRLEHNVITRSLFEAVI
                     VNDQAQAVLVGNTFVKNGGGAAFLGTSQNEASGNIVSLNTYGFVVAASSRVLFSYNAM
                     QNSGSNYLRSGTPNQPAPELKPDSDLTVDPRFVDTAQDDFRLRADTSLVKIGTFPYLG
                     ALPPLSASR"
     gene            11120..11305
                     /locus_tag="PLS35_11775"
     CDS             11120..11305
                     /locus_tag="PLS35_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19392.1"
                     /translation="MASFRFDLEKRSTDGIPNLAEVESGKLLGAILPMSEQAQLQLRD
                     SIEVIDYLMNQETVVWS"
     gene            11585..>13442
                     /locus_tag="PLS35_11780"
     CDS             11585..>13442
                     /locus_tag="PLS35_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNI19393.1"
                     /translation="MSTSTGKKSVPVPPSAEQQRLDDDDLRRKHWKRWGPYLSERAWG
                     TVREDYSPYGTAWEFLPHDHARSKAYRWNEEGLAGISDRHQFICLALALWNGRDPILK
                     ERLFGLTGNEGNHGEDCKEYYFYLDSTPTHSYMKFLYKYPQAAFPYTRLIEENRRRNR
                     TDPEFELLDTGLFDDDRYFDIVVEYAKASPEDLLLRITAINRGPEPSELTVLPTIWFR
                     NTWSWGIDVRRPRMRQGESSPGVSAVEFDHEYYGRRRFLCDGAPDLLFTENETNTRRL
                     YGDSDGARYVKDGINDYIVQGDKNAINPDRIGSKAAAHYVRSAMPGQPVTIRLRFTNG
                     SAEGALDPEAFDAVFAARLKEADEFYAHLAPPDLSADARLVQRQAFAGLLWSKQFYHY
                     EVDRWLKGDPAGPEPPPERLRGRNADWTHLYNADVISMPDKWEYPWYAAWDLAFHCLP
                     IALVDSRFAKDQLVLMLREWYMHPNGQIPAYEWALGDVNPPVHAWAAWRVYKIEKKRK
                     GVGDRVFLERVFHKLLLNFTWWVNRKDAEGKNIFQGGFLGLDNIGVFDRSKPLPTGGH
                     IEQSDATSWMGMYCLNMLSIALELARDDRAYEDVASKFFEHFVYICRAMNNIG"
BASE COUNT         2615 a         3863 c         4104 g         2860 t
ORIGIN      
        1 atgagccatc cctaggttgc cgggcgaaac cgcagcgtac acgtggtgcc ttttccttgc
       61 ttactggtca cgatcaaggt cccgccgttg tcttccacga gccgtttgac gttggtgagg
      121 ccgagtccgg tgccgtaggt cttggtcgtg aaaaatggtt cgaagattct cccaagatga
      181 tcgggagcga tgcccgatcc tgtatcggtc acgttgattt ccaccgtgcc tccttccgcg
      241 ctgcgagtcg tcagggtcag agtccctcct ccctccgtca tcgcttcccg ggcattaatc
      301 agcacattca ccaacgcatc tttgatctgt tcaggatcga tgcgaatcat gggcagatcg
      361 tgtgagaacc gctgttcgac ccggatatgc tcatggccac cttgcgcctc ccattgcttc
      421 agcgcagcgc caatgatccc ttccgcagca cagtcggcca ggtccggcgg tttctgtctg
      481 gcataacgca aaaagtcgtt cagtcgctga gacagcttgt cgacctcgtc gatgatatag
      541 cgggccattt catgccggat tccttcttcc tggggaccct ccagcacgac ttgcgcggaa
      601 cttcgaatca caccgagagg attgcgtagg tcgtgagcca gagcggcaag gagttgtccg
      661 acggaggcga gcttttcctg ccggatcaat tcatgttgtt gcgccctgat agtgcgcaga
      721 ttttcttcca gcacggctcg catcgcgctg aacgtggtgg caagatcttc gatctcatcg
      781 ccggtgcggt attctcgggg caagggcgta gcagcgaggg ggagagcgtg atcagtctga
      841 gtcgtcgcca gcgtgaggcg cagcccttcg gcctcccgct gaagcgccgt gagtggtcgc
      901 acaatacggt agccaaccac aaatccaaac atggacaacg cgatcaccag cccgaagccg
      961 atcacgccta ccgacaggag gagcgaatag atcggcgcat aggtttcttc gggagcctgc
     1021 cggacaaagg cataccatcg attgccttcc aagctggacc gggtcaacgg atggctgaag
     1081 cggaccggcg aggctcccac gattgcgtca cggccgccgt gagcatcgtc ctcggccacc
     1141 aaccataagg gatgatccag agccagccgg ttcatcaggt tcgcgggaat caggtgggca
     1201 gtcggcggca gcaccggaca gatgagcggt gtcccttgcg tatcgaggag catcgcatgg
     1261 cctgtattgc cgatatggat gggaagaatc agctgggtga ggagcgtgtg ccgaatcacg
     1321 aggtctacgg caccggtcac cgcgcctcct gccttctcga caatgggaac cgcgacgtgg
     1381 aaggtcgcgt cattgatggc aggatcgatc gtgacgttgc tgacataggc cgtcccggcc
     1441 ggggcctgca tggcctcgcg ccaccacgat tgatcggcct gctgcggtgg caaggcccga
     1501 tcggtggagg cgatgacgag gcctcggtga tcggccacgg tcacgcgcag ataatgccgc
     1561 gcttcgcgtg cccaatcttc gaggtaggcg gcggtgacgg ggctgttgac aggccgatgt
     1621 gtggaatgaa cgcgggcggc ttctgtcttg ttgctgtcgg ccttggattg agcttgtgcg
     1681 tgctgattcg ccacgctgat ggcgaggcgg ccggcgcgat cgatttcatc gtcgacggcg
     1741 ccggccaatc gaatcgaggt cgagcgagca atttcctgga acccttcgcc gatcgcctct
     1801 ttgaggctgg ccgtgctgga gcggtacgtg gcccacagtg ccgccatgcc gggcacgatg
     1861 ccgacgatca ataacgcgac aaaaaatttc ctctggagtc cgccggtccg tcgtgcagcg
     1921 cgcatcaccc ttgaggctgc gacaggttat gtttcgccat gcgataccgg agcgtgttgc
     1981 gcgtgatttt gagcagccgg ctggcttcgg acacattgcc gccgctcttg gccagcgcct
     2041 cttgcagcat cgccttttcc acctcttcga ctgacaaccc taacgacagg agcgaaggag
     2101 cgccggccgg cgacgcagac gattggggtg cggcggcggt caaggaggca gggagatgtt
     2161 cgggctgaat catgtcctgc ttgcaggtga tggtgagcca ttccacgacg ttgtgcagtt
     2221 cccgcacgtt gccgggccaa gggtactggg tcagaagcgc gagcgcattc tgagcaatgc
     2281 ctttgatccg gctgccacgc tctcgccggg ctcggtcgag aaactgagtc aggatcggtt
     2341 caatgtcacc cgggcgctcg cgcaagggcg gcatgcgaag ttgatagacg ttgaggcgat
     2401 aatacaagtc caggcggaag cggccggcct tgatgagatc gggcaactcc tgattggttg
     2461 cggcaatgac tcgcagatcg accggaatac tccgcacccc gccgagcggc tcgactacat
     2521 gctgctccag cacccgcagc agtttggcct gcgcggtcag actgagttcg ccgatttcat
     2581 ccaggaacaa ggtgccccgg tctgccattt gaaagcggcc cggtttggat tttttggcat
     2641 ccgtgaaggc gcctttttca taaccgaata attcggattc aagcagatgc tcgggaatcc
     2701 cggcgcaatt cagtgccacc atcgggcctt gggcacgcgg actggccaaa tgaatcgctc
     2761 gcgcgagaag atctttgccc gtgccgctct cgccggtgat caacaccgtg gcatcggtct
     2821 gggccacctc cttggccagc cgtttcacga gctccatttc tttggagacg ctgacgatcc
     2881 ggtcgaatcc gaactgctcc tgcagccctg cgcgtaaggt gcgattgtcc cgggcaagtt
     2941 gacggacttg cagtgcccgg gcgatgacga ttttcagctc ctcgttgtcc accggcttgg
     3001 agatgtaatc gtaggcgccc ttttgcatcg cttcgacggc cgacttcacc gtaccgtatc
     3061 cggtcacaat caggaccggc acatcgggcg cctgtttatg cgcggcttcc aggacgtcca
     3121 tgcccgagac ggttcccagc tgtagatccg tgatgatcat gtcgatcgaa tcatgctcct
     3181 ggagcatgtg aagggcttcg tcgccggaac gcgccagcag gagttgatag ccggctgctt
     3241 tgagcaccag ctcaaagagc cgccgcatgc gttcttcatc gtcgacgatc aggagggtgg
     3301 gggcgtgcat ggtgtcgcaa ccgctgaggt gggtccccga ctcggcgagg acgtgagtcg
     3361 tagcctacca ctatcggcga aagagaaaca gaggggagct taccgggaat cacggctcga
     3421 tgatgaggcg ggcgtgccat ttccggctgg cgcggcttca ttggccagtg cccaggagac
     3481 gttgcgttgg agtccggcat gtttggcgcg tcgaaccgga ctctgtttga acgtcgccgc
     3541 gaaggttgcc tcgttcatgt ccatcaagct gcgcaacgac ggtgaggtgg tgagcggtgt
     3601 gggctggaag cccggctcgc tggtcggttc ggcctgatga ttatacggac agatatcgag
     3661 acaatcgtcg cagccgaaaa tccgattgcc gataccccgt cgcagctcct cagaaagcgt
     3721 gggctcgctg cctcgaagtt caatcgtgag gtacgaaatg catcgcccgg catccacgac
     3781 atagggctcg gtgatcgcac ctgtcggaca ggcttggatg caaagcgtgc aactgccgca
     3841 caaatcggtt ccggcctcgt ccgcatcgag ctccagggtc gtcagaattt ctcccaacag
     3901 gagccatgaa ccaaactcag gggagaccag gttggagtgc ttgccgatcc atcccaggcc
     3961 ggcctgctga gcccaggctt tttccatgac cggccccgta tcgacgtagg cgcgatggcg
     4021 atcctcaggt gccagagtgg cgagccgttc agacagctgc gttaaccggt cacccatgat
     4081 gttgtgatag tccagtcccc aggcataccg ggcaatccgc ccgttgcccg gtcgttcatc
     4141 ggcccgatga tccgtgtagt aattcattcc gacggaaata atggaccggc atccgggcag
     4201 gaccagtgcc ggatcggccc gtcgttccgg ggcgcgcgtc atccagttca tggaggcatg
     4261 gaagcctcgc tgcagccatt catgtaaccg gtcgaggagg cggtggggag aaacgctggg
     4321 attgtggttc ggtgccggct gtggtggcaa ccggctgatg ccgacgacat cgaatcccag
     4381 ctcgcgcgcg gcttgtgtaa tcagcgctgt gagtggctcg ggcattacgg tttcgcgcac
     4441 tcgaaacgca ccatgaattg gccggtgcaa tggctcgaat cgcagcgggt gcattgaccg
     4501 gtaatcttgt ttttccagtc cgtccctttt ttgtcgaagg cgcagaaggt gcgaccggct
     4561 ttggagacgg tcgtccaggg tttgtctgtc ccgggaaatg ttgcgatgac acagccggct
     4621 ggaattggta cttggcactg atgggccgga tcacccttgg ccatgccgaa actgcaggag
     4681 tgttcggtgg tggccgcaga gtcagcaggt gcttgtgcct gcgcgtcgaa cggcatgaca
     4741 agaagcgcaa tctgcacact gagtaggcca ggggaaatca ggcggacgag ccaggacatc
     4801 gtagagcgct ccagtgaaaa gggcatggta ccacagcctt gtttctgcgg cgaaccctca
     4861 tctccggcat gccccgctat ttccggccct gatcttccga ttctgtccgg acattgttca
     4921 aaaaccaccg tcggtgtagg gagaagcagg aacgcgaggg cgtaacttgg tgcaagaaca
     4981 ggatcgggca agtggcatgt tagttgcgag aaccggtaga ccactagtgc gtgtgcatag
     5041 gtgtgcgacg ggaccacgag atcccggcat gacgggcaac aatcctgtgt tcaagccgtt
     5101 ttctgattgc accatggctc aggaaaaaat tttgattgtc gatgatgaag tggtggtggc
     5161 tgaggacatt cgtcgccagc tgcggtcgct ggggtatgtt gtcgtcgggg tggtggcctc
     5221 aggaagcgag gccgtggata tggctggaga gcatcggccg gacctgatcc tcatggatat
     5281 caagctcaaa gggccgctcg acgggatcga tgccgctcgc atgattcaag cgcgctacgg
     5341 cacgccggtc atctatctga cggccttctc cgatgaggac acgctgaagc gtgcacggta
     5401 tacattgcca ttggcgtatc tgatcaagcc ttttgtgagc agtgatctgc gcgctgcgat
     5461 cgaactggcg ctctttcgtc atcgtgtgct gcgtattgcc gagcagcgcg gccgctggct
     5521 tgattcggtg ctgcagtcga tgagcgaggc ggtcgtgaca atcgatgctc agggaaaggt
     5581 gacgttgctc aaccaggcgg cggaggggct gaccggctgg tcccaaccgg acgcgctcgg
     5641 aaagccgatt caagatgtca tggtcgtggt ggatgcggag cggcgcacgc cgctcgtgaa
     5701 tcccgccctg tcagtgctgc agcagatcaa ggctggggat cggggcacgc gcccgttcct
     5761 gctcctgagt cgtcaaggcg tcgagcacgt gatccacgag agcgcgaccg tgatccacga
     5821 tgaacaaggc ggcctgaccg gagctgtgct ggtattccgc ctcgccatcg agtagctatt
     5881 ggggaccgcg gctcgctgcc gtcagtcacg gatttaccga tcgcagaaat cggtatcggc
     5941 ggtcattccg tcatgctctg cactcgcact ccaggcagtc aatcagtata cttatcctat
     6001 gacctcttcc acggtgattg gttcatcggc ccgtccgttt cctggagtac ccatgattcc
     6061 tcgtgccacc gtcgaacgcg cctcgatccc cttcgtaaca cccagcgttc tgcgactcgc
     6121 caggcccttc ggtagagact gatgcgtgcc atggtacagc tcatgtcttt tctgggaacg
     6181 ctgctgggct gcttgatggc attcgtgttg gcgaccaccc ctcaatcctg ggcaggagag
     6241 gtcgggtcga tccatttgct tcggcccctt ctggtgcagt ctgaaccggg tggcggggtc
     6301 aaagccaccg ccacgctgct cttcccgggc agtccctccg tcctgcattc cattctcacc
     6361 gaatatcgca agtggcccga gctatttgaa acccgtatgc gcatggcaca ggtggaggag
     6421 cgcgaaggcc atgtcctgac ggacgtctat atttcccatg cgctgctgcc aggcgagcag
     6481 cgattgctct gtgagtcgca ggtgttaccc ggcggcgggt tggtgaccga tctcaaaggc
     6541 ggagatttca agcagtacca tcgcgagtgg aggctgcagg cggcaggtga aggcactcaa
     6601 acgagagcgg agtttgaatt gctcgtcgag cccaagacca tgattcccga ttggttgatc
     6661 gccgttgcca tggagcggga gctcacggtg cactttcgtc tcctgcggca acgggcgctg
     6721 gatctggtta cgaagggaag gtaaaggtcg tcagttcggc cttctcgacg cctcgcttga
     6781 cgaacgcgct gatctccagc cctcgttcga tgcggacgat atcctccaat ttggttctgg
     6841 tgcttggatg gagggggacg aacagtttgc agcaatcctg atcgggttca atggatgtgg
     6901 tgaagctgcc gatctgttgg gcctgttccg tgatctcgat tttatccatg ccgatgagcg
     6961 ggcggagcaa aggaatctcc gccgcgtttt cgacgaccac cagattctcc gctgtctgcg
     7021 aagccacctg accgagactg tctccggtga ccaatgccca gcagccttcc cgccgcgcca
     7081 gctcctgtgc aatctgcacc atgatccgcc ggtataacac cacacgaaac gccgccggcg
     7141 cattggcgac gatctcctgt tgaatttcgc cgaacgggac gatgtagagc ttggagtgat
     7201 attggtacgt cgttaaaagt tggaccagct cctggacttt ttcctccgat gcgcggctga
     7261 ccagcggccg gccggaaaaa tggacgaaga ccgccttgca cccgcgcttg atcatgcggt
     7321 aagccgcgac cggggaatcg atacctcccg agatcaaaca ggcgactttg ccgctggtgc
     7381 ctaccggcat gcccccggga ccttcgattt tcctcgctgc aacatgggct tccttggtga
     7441 ggagctcgat gtagagggtc acgtccggct gtttcagctt gacggctttc cccatggctt
     7501 catggagata ggcgccgact tcgcgctcca catccatcga cgtcaacggc aggcgtttat
     7561 ccgcgcgttt ggccgttact cgaaacgagg taaacgtctg tgtcccaagt tgacgctgcg
     7621 caacctgctt gatgctcgac aggtccggct ccctgatgtc taacggcacg gattcgctca
     7681 gcaggaagtt tgagacgccg caaatgcgac ggagccgctc cgtcaccagg tccggagtga
     7741 cgtcctcggg aatcgtcacc cggatgcgtc cctgcagcga ttcgatccgc cggatcttca
     7801 ggtctttgag ggccaggcgg atgttgcgga cgagccgctg ctcgaagaaa tcgcggttac
     7861 ggcctttgag ggcgagttcg tggtagtgaa tgatcgcgca atgcatagtc ccggtcgctg
     7921 aaaacttcct ccggccgcgt tctcagtcgc ttgtccttcc tcacgtaccc actcgtacgc
     7981 tcggtcggcc aagctccctg cggccttgcc ggagaaattt ttgagcgacc tccttttaat
     8041 taagttcggt attggtttgt gaagaattgc cgatcgtcga ctgctggcaa cttcgttctc
     8101 ggctcgacga tatcctcaac gtaccactgc gggtacgcct ccggtatctg tctcgcctgc
     8161 ggcctcgttg gacaaccatt ttgagcatcc tgcgggaggt ttagagaggt tagagagaat
     8221 gaagcgctga ttctagaaac cgttcggcgt cgatggcggc catgcagccg gagccggctg
     8281 ccgtaatggc ctgccggtat ttcgcatcct ggacatcgcc tgcggcaaac acgccgggca
     8341 cactggtgtt cgtcccgcgc gaggtgagga tgtagccctg ctcgtccatc tccaattgac
     8401 ccttgacgag atcagtgttg ggacggtggc cgatggcaac gaagacgccg gcgcagggga
     8461 ggtctgtgga atggcccgtc acgaggttct tgagccggac accggtgacc agatctttcc
     8521 cgagaatgtc ttccacgatg gagttccagg caaaggagat cttttcattg ttcatggccc
     8581 ggtcttgcat gatcttggag gcacgcagct tatcgcggcg gtgcacaagg gtgacggtgc
     8641 gcgcaaactt cgtcaggaag gtcgcttctt ccaccgcgct gtcgccgccg ccgaccacga
     8701 ccaaatcctg gccgcgaaaa aaatatccgt cgcaggtggc gcaggtcgag acaccatacc
     8761 cggtcagacg gtgttcgttc ggcaagccga gtcgaatggg ggaggcgccg gtcgccaaga
     8821 tgagagcgct cgccccgatc gtttgttctc cgtcgatggt caggcgaaac ggtctggtcg
     8881 acagatcgat cgcggagacg tcgccggtca ggaactctgt cccgaatcgt tcggcctgac
     8941 cacgcatctc cttcatcaac tccggcccca taatcccttt cgaaaacccg ggatagtttt
     9001 cgacttccgt cgtggttgta agttgtccgc cggattgcca gccttcgatg agaagaggcg
     9061 acaggttggc ccgcgcggcg tagatggccg cggtcagacc tgcaggtccg gagccgatga
     9121 tcacgagatg atgcatacgc ggatgctaac acaccgatgc tgcgagggaa aagccagagg
     9181 cacggcattt gggtaacgat tcgcacggat cacggatgac cgaaggctgc tgggtcgcag
     9241 atccgctagg cgagcctggc caattcgcga tagagcgatt tgaccagcgt aaacaggcgc
     9301 gggcgggcgg tggttccgtc gaggacaaag tcggcggccg ccactttttt cctcaggggc
     9361 cattgattac ggatgcgtcg gagggcttcg ctgcgggtca atccattgcg tttcttcaat
     9421 cgggcgatct gggtttcccg atcggcggtg accacgatga ccttgtccac gcgcgcatca
     9481 atgccggctt cgtagagaag aggaacgtcg tagatgacga cggcgtttgg atccttgcgg
     9541 acggccagcc tggtaagtct cgcctgttct cgcgcgacgc gggggtggat gatctgttcc
     9601 aaacggcgca gcctcgcgcg gtttcgaaac accatggttc ccagagccgg gcgattgatc
     9661 gttcgatcgg gattgatgac ggcgggtccg aacgtcttca cgatcgcgcg ccaggccggc
     9721 ttacccggct ccatcacctg atgtgccagt gcatccgcgt cgatgacgag agccccacat
     9781 cgttccaaca tcttggcgac cgtgcttttc ccggtggcga cgccgcccgt gaggcccaca
     9841 aaaatcatag ggcgcgaaga taccatgggg gtccgatccc cgcaacgagg ctcggttgac
     9901 ttctcgctat ggccagcggt atgaccatcc tatgaaaata atgaactatt ggatattgca
     9961 gatcgcattt ctctgtgtct cggtcggcac ggtgagcatg gtctccgtga aagcggaaga
    10021 acctccagca gaacggcagc cgctagtcgt ggccttggac ggctcgggcc agtatcgctc
    10081 cattcaggaa gccgtggatg ccgcgaaaaa aggtgacacc atcctgattc gccccggcgc
    10141 ctaccccgaa gatgtgacca ttcacagtaa agaaaatgtt cgcttgatcg gggccggcat
    10201 ggatcaggtg acgatccttg gccgtgaacg ggtgggcgtg tttcacatcg gcaaatggcc
    10261 ctatggcgcg acgaatatcg agatctccgg catcaccgtc aatgaacatg gcgggcatgc
    10321 gatgggcatg tttaatggcc gtggcatcca ctttcatcat gcgcgggtga aaggcatgct
    10381 gtttacccag caagtggagg atgtgcggat cgagaattgc gatatcggcg gcagcgaaac
    10441 caccggtgtt caatttgcca actcgcaggc cgtcatgcgg gggaatttca tccacgacaa
    10501 cgaccacggc gtcagtgtcg cgggaaaatc caccgtgcgg ctggaacaca acgtcattac
    10561 ccgaagcctg ttcgaggccg tgatcgtcaa tgatcaggcg caggccgtcc tggtggggaa
    10621 cacgttcgtg aaaaacggcg gcggtgcggc atttttaggc acgtcgcaaa acgaagcgtc
    10681 ggggaacatc gtgagcctca atacctacgg cttcgtcgtc gcggcctcaa gccgtgtcct
    10741 cttctcctac aatgcgatgc agaattcagg ttccaactat ctccggtccg gtactccgaa
    10801 ccagccggcg cctgaactga aacctgattc tgatttgacg gtggaccccc gtttcgttga
    10861 cactgcgcaa gatgatttca ggcttcgagc ggatacgtcg ctcgttaaaa tcggcacctt
    10921 tccctatctc ggcgcgctcc cgcccttgag cgcatcgcgc tgaacttcag tcggaacgcg
    10981 caacaaatta agaggttacc tggctcgtct tagcgacgga ggtcctgtgc tatgcttcat
    11041 ggtgagccat ggacgtatag ggcgcctgtc tggctcaaga attgctgcac tacgaccgac
    11101 aaaggattgg aggtaagacg tggccagctt tcgcttcgat ctcgaaaaac gcagtacgga
    11161 cgggattccc aacctggcgg aggtggagtc cggtaagctc ctgggggcca ttttgccgat
    11221 gtcggagcag gcgcaactcc agttacggga tagcatcgag gtcatcgact atctcatgaa
    11281 ccaggaaacc gtcgtctgga gctagtgtcc ctgcctcctc gcgcaagcaa tcctcccgcg
    11341 ctccttcctg tcccgcattt cattgtatgt cccgctcatg cagttcaccg tcctactcac
    11401 ggaccggtgc tgctacacgt tccattcgtg atcgaccgtt ccattcactc ggctctccac
    11461 tcctccgaac atcagcagtg atcctcctca atccttcacg aatccctgtt gaatcttttt
    11521 gatgcccggt gcatgctggc gccgttttgg gtcaaggacg gccagcaaga ggagtctgtg
    11581 tcatatgtcg acatcgaccg ggaagaagtc tgttcctgtc ccgccatcag ctgaacagca
    11641 gcgcctcgat gacgatgacc tccgccggaa acattggaag cggtggggcc cttacctcag
    11701 cgaacgggcc tggggaacgg tccgcgaaga ctatagtccc tatggaacgg cctgggagtt
    11761 cctgccccat gaccatgccc gctcgaaggc ctatcgatgg aacgaagaag gattggccgg
    11821 catttctgat cgccaccaat ttatctgtct cgcgctggcc ctctggaacg ggcgcgaccc
    11881 gatcctgaaa gaacgcctgt tcggactgac ggggaatgaa ggcaaccacg gagaagattg
    11941 caaggagtac tacttctatc tcgattccac gcccacgcat tcctacatga agtttctcta
    12001 caagtacccg caggctgcct tcccgtacac gcgactcatc gaagaaaatc gccgccgcaa
    12061 ccggacagac ccggaattcg agctgctcga caccggtctg tttgacgacg accggtactt
    12121 cgatatcgtt gtggagtatg ccaaggcgtc gccggaggat ctgttactgc gcatcaccgc
    12181 catcaatcgc ggcccggagc cctcggagct gacggtgtta ccgacgatct ggttccggaa
    12241 tacctggtcc tggggcattg atgtgcgccg cccgcgaatg cgccagggcg aatcgagccc
    12301 cggggtgagt gccgtcgaat ttgatcatga atattacggg cggcgtcgat tcctctgcga
    12361 cggagccccg gatttgctgt ttacggaaaa tgagaccaat acacgccgtt tgtatggcga
    12421 ttccgacggg gcgcggtatg tgaaagacgg gatcaatgac tacatcgttc aaggagacaa
    12481 gaacgccatt aatccggacc ggatcggatc caaggccgcc gcgcattatg tgcgttcggc
    12541 aatgccggga cagccagtca cgattcgcct gcgctttacc aacgggtctg cggagggagc
    12601 gctcgatcct gaggccttcg acgccgtctt tgcagcgcga ttgaaagaag ccgacgagtt
    12661 ttacgcacat ctggcgcctc ccgatctgtc cgccgatgcg cgcctggtgc agcgccaggc
    12721 ctttgccggg ctgctctgga gtaaacagtt ttatcattat gaagtcgatc gatggctcaa
    12781 gggggatccg gccggtccgg agcctccgcc ggagcggttg cgcggccgaa acgccgactg
    12841 gacccatctc tacaacgccg acgtcatctc catgccggat aaatgggaat atccctggta
    12901 tgccgcctgg gatcttgcct ttcactgcct gccgattgcg ttggtcgatt cgcgatttgc
    12961 caaagaccag ctcgtgctga tgcttcgtga atggtacatg catccgaacg ggcagattcc
    13021 cgcctacgaa tgggccttgg gcgatgtgaa tccgcctgtg catgcctggg cggcctggcg
    13081 cgtgtacaaa atcgagaaaa aacgtaaggg cgtcggggac cgggtctttc tggaacgggt
    13141 ctttcacaag ctgctgttga actttacctg gtgggtcaat cgcaaggacg ccgaagggaa
    13201 gaatatcttt caaggtggat ttctggggtt ggacaacatc ggcgtcttcg accgcagcaa
    13261 gccgcttccc accggcgggc acattgaaca atcggacgcc acgagttgga tgggcatgta
    13321 ttgcctgaac atgctgtcca ttgcgctcga actggcgcgt gacgatcgtg cgtatgagga
    13381 tgtcgcgagt aagttcttcg agcatttcgt ctacatctgc cgggcgatga acaacatcgg
    13441 ca
//
LOCUS       DAOQEW010000103        13402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1327_length_13402_cov_8.927999, whole genome shotgun sequence.
ACCESSION   DAOQEW010000103 DAOQEW010000000
VERSION     DAOQEW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_13402_cov_8.927999"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..562)
                     /locus_tag="PLS35_11785"
     CDS             complement(218..562)
                     /locus_tag="PLS35_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNI19394.1"
                     /translation="MDNCIFCRIVNRAIPAKIVHEDDRVLAFEDVNAQAPVHILVIPK
                     RHLAAVQNCQDGDQALLGHLLMTCSKVAAMKNLAESGYRIVTNTGADSGQTVFHLHLH
                     VLGGRHMAWPPG"
     gene            complement(555..1238)
                     /gene="hisIE"
                     /locus_tag="PLS35_11790"
     CDS             complement(555..1238)
                     /gene="hisIE"
                     /locus_tag="PLS35_11790"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNI19395.1"
                     /translation="MSSDSRSMTFDGQGLIPAVIQDWFDGTVLMVGYMNQEALTKTLA
                     TKSVHFWSRSRQKLWEKGETSGHFLRVKDLFVDCDRDTILVKAEPVGPTCHTGERACF
                     FSRMSADGRAEPGKTQDAGGGILERLYQTILARKAAPQPDSYVSKLLQGNVDRILKKV
                     AEEAGEVIIAAKNQKRDEIIYETADLFFHTLLVLGYHDVTLSEVYRELGARFGKSGIR
                     QAPEGESRG"
     gene            complement(1251..2021)
                     /gene="hisF"
                     /locus_tag="PLS35_11795"
     CDS             complement(1251..2021)
                     /gene="hisF"
                     /locus_tag="PLS35_11795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNI19396.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVEVATIYDREGA
                     DELCFLDITASHENRKTIVDVVERTAARVFMPLTVGGGVRTLDDIRTLLNAGADKVSI
                     NTTAVQKPEFVREAAQRFGTQCIVVAIDAKRAEAAGRWQVYTHGGRNATGLDAVEWAQ
                     RMAEYGAGEILLTSMDQDGRQNGYDLALTAAVSESLSIPVIASGGVGTLEHLYDGLVK
                     GKAEAVLAASIFHYRTHTIQQAKAFLRDRGVPVRIGAA"
     gene            complement(2092..2817)
                     /gene="hisA"
                     /locus_tag="PLS35_11800"
     CDS             complement(2092..2817)
                     /gene="hisA"
                     /locus_tag="PLS35_11800"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNI19397.1"
                     /translation="MRVIPAIDLKDGRCVRLRQGDMAAETVYSEDVPSVAKRWQEQGA
                     DLIHIVDLNGAVDGEPKNLPQIESAMKAVNVKVQVGGGIRTIDTVRKYLQAGVARVVL
                     GTAALTDRAFLAQACREFPRRILLGLDARDGKVAVKGWTAVSDTKAIDLLRELADHEL
                     GAVIYTDIARDGMLNGPNLAALGEVVNCAAFPVIASGGITRVEDLQAVRALGPRVEGA
                     IIGKALYDGKLDYRAALAALGNR"
     gene            complement(2840..3577)
                     /locus_tag="PLS35_11805"
     CDS             complement(2840..3577)
                     /locus_tag="PLS35_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19398.1"
                     /translation="MSTCIPRTLFILLSVVLTTGCSGTKVTSKASPQAGKYQVRNIVL
                     IPFETMATPQAIETSGPSFPVPSGVRRSDMAVGVPPTTDQAVRRTNLVPPGAGEKITD
                     LMWAKLKGKPGLQVISPQEAMGAARDLSRGSGSVETIPAHKIAQRLKADAALSGKVLV
                     YQERVGSRLGADPPAAVGFEVKLVAPDGMVLWEGNYYEKQRPMTEDVLGFVQRYGTFV
                     TAEELAAYGATELAEAFPFGAAAGEGK"
     gene            complement(3574..4182)
                     /gene="hisH"
                     /locus_tag="PLS35_11810"
     CDS             complement(3574..4182)
                     /gene="hisH"
                     /locus_tag="PLS35_11810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNI19399.1"
                     /translation="MIAIIDYGMGNLRSVSKAFEAVGHQAVVTREARVIADASHVVLP
                     GVGAFGDCMANLERYELIGPIHAAIQSGKPFLGICLGLQLLFAESEEFGTHKGLGIIP
                     GRVKRFVLDASLKVPHMGWNDIRIMRPAPIFAGIAPGSYCYFVHSYYVEPADSAVVAT
                     VTDYGAPFASAVWKDNVVACQFHPEKSQAVGLQVIKNFGAWT"
     gene            complement(4247..4852)
                     /gene="hisB"
                     /locus_tag="PLS35_11815"
     CDS             complement(4247..4852)
                     /gene="hisB"
                     /locus_tag="PLS35_11815"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNI19400.1"
                     /translation="MKNTGVPRQASLHRATKETDIRVEWTLDGTGQGKIDTSIRFFDH
                     MLDLLAKHGFFDLTVQAKGDIDIDEHHTVEDVGIVMGKALHQALGEKAGIKRFGWASV
                     PLDETLAQVTVDLSGRPYLVYNVNLPDRKIKTFDLGLFEDFFQAFVTHGGLNLHINLQ
                     YGRNPHHIMEAIFKALARALDQATLPEERLSGAVLSTKGSL"
     gene            complement(4849..6132)
                     /gene="hisD"
                     /locus_tag="PLS35_11820"
     CDS             complement(4849..6132)
                     /gene="hisD"
                     /locus_tag="PLS35_11820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNI19401.1"
                     /translation="MTILESTDRAFAKALRKITARSRSQAATVEKSVRAILQAVEKGG
                     DRAVLRYTKKFDRVSVTLDQIRVTPEEIKEAYYHIRKDEGDSLRYAAERVRQFHERQR
                     TKTWMYQEENATLGQMVTSLDAVGVYVPGGKAVYPSSVLMCAIPAKVAGVRRIVMCTP
                     TPKGEINPYLLVAADIAGVDEIYRVGGVQAIGAMAFGTKSIAKVDKIVGPGNMYVATA
                     KRLLYGTVGIDMVAGPSELLVVADDDAKPAHVAADLLCEAEHDEDAQVYLVTTSASLA
                     KEVVRLIQVQLKGLQREKIAAKSIARHFVAFVVPTMDEAIEVANDIAPEHLTLSVDRP
                     FDYLEQIRHAGALFLGRYTPPAVADYVAGPNHVLPTGATARFFSPLSVNDYVKVSNIV
                     HYTREELTKAKDHIVRLAHIEGFDAHAKSAQSRFS"
     gene            complement(6218..6886)
                     /gene="hisG"
                     /locus_tag="PLS35_11825"
     CDS             complement(6218..6886)
                     /gene="hisG"
                     /locus_tag="PLS35_11825"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNI19402.1"
                     /translation="MKARKSQDEKPKGLTIALSKGKLLGPVLHLMKAAGYYSPGLSVE
                     SRKLVFDCAANNATFLIVRPTDVPTYVEHGAADVGVVGKDVLLEQGSDVYEPLDLKVG
                     ACRISVAALQGQPSPDRWSSKVRVATKYPNVTERYFNQRGMPVEIVKLYGSIELAPIV
                     GLADRIVDLVETGSTLKAHDLAEVEVITHSTARLIVNRASLKLKHEPLQVLIDRLRTA
                     VAAE"
     gene            complement(6889..8145)
                     /gene="murA"
                     /locus_tag="PLS35_11830"
     CDS             complement(6889..8145)
                     /gene="murA"
                     /locus_tag="PLS35_11830"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNI19403.1"
                     /translation="MDQILIQGGRRLQGEVRTSGAKNAALPILASTILGGGECVLSNM
                     PRVVDVLTMGKLLRMLGIAVSQEGDQTVVQAEAIASTEAPYDLVRTMRASVLVLGPLV
                     ARLGEAKVSLPGGCAIGSRPVNFHLAGLEKMGATVEIEHGYIKATARRLRGSRIYFDT
                     PSVTGTENLMMAAVLAEGTTVLENAAKEPEISDLAAFLVKRGARIAGAGTDMITIEGV
                     TELHGADHEVIPDRIEAGTYLVAGAITGGEVIVDRCRPEHMEPLLMKLRESGAELIEE
                     KERVHLKVTGRLKGTNVRTSPHPGFPTDMQAQFVALMAVAEGTSVVTETIFESRFMHV
                     EELRRMGADIRVEGNRVVITGKERLTGAPVMASDLRASAGLIVAGLAAEGTTEVSRVY
                     HLDRGYERLEEKLRILGASIERRKGK"
     gene            complement(8189..9115)
                     /gene="prmC"
                     /locus_tag="PLS35_11835"
     CDS             complement(8189..9115)
                     /gene="prmC"
                     /locus_tag="PLS35_11835"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNI19404.1"
                     /translation="MRQQQLADAACSVSSLGELLRKSESRLDAAGIDQPALESAWLVE
                     HVLKLSPLMQRINTERGLTPEECEGVQVLVERRANREPLQYVLGTQEFCGRDFFVTPS
                     VLIPRPESVLLVEEAVRRCVQISDATIVDVGTGSGCLAVSVASALPTARVLAIDASTD
                     ALVVARNNMARYGVEARITCLQGDLLDPIGQEGLPNQVEVILSNPPYIPDGDVETLQP
                     EVRDFEPRLALAGGHDGMDVHRRLIAQAPAYLKPGGALLMEVGIGQAAAVCRHAEEGG
                     RLRTYVVLRDQGGIERVVCFEKKEESLSSSSS"
     gene            complement(9112..10251)
                     /gene="prfA"
                     /locus_tag="PLS35_11840"
     CDS             complement(9112..10251)
                     /gene="prfA"
                     /locus_tag="PLS35_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNI19405.1"
                     /translation="MGQGLLFFGLQGYGRNGRDMEDGLIKKWEAMAARFQELTNQLMD
                     PTVLNQPGMIHKLNKERTELEEPAQEFAAYSQAQKQLHEVTVMLQDSGTGADMKQLFA
                     EESEHLTQQLLDIEARAKDFLTPKDPRDEKNVFLEIRAGTGGDEAALFAGDLFRMYLK
                     YAERNRLRLEVVDASETGIGGYREVIALLEGKGAYGRLRYESGVHRVQRVPTTEASGR
                     IHTSTVTVAVMPEVDEVDVHIDPKDLRIDTFCSSGAGGQSVNTTYSAVRITHIPTGVV
                     VSCQDERSQLKNRTKAMRTLRARIVEAEREKQEAAIAQDRKSQVGTGDRSEKIRTYNF
                     PQNRVTDHRVGLTVHKLDQVLAGDLEEIVQALRAHYEHLASLETK"
     gene            complement(10288..10494)
                     /gene="rpmE"
                     /locus_tag="PLS35_11845"
     CDS             complement(10288..10494)
                     /gene="rpmE"
                     /locus_tag="PLS35_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNI19406.1"
                     /translation="MQKGIHPVYREATVHCACGNTFKTRSTIGDIKVDICAGCHPFFT
                     GTQKIVDTEGRVERFKKKYAKKDK"
     gene            complement(10588..11835)
                     /gene="rho"
                     /locus_tag="PLS35_11850"
     CDS             complement(10588..11835)
                     /gene="rho"
                     /locus_tag="PLS35_11850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNI19407.1"
                     /translation="MHLAELKQKSIADLNDVARDLKIEGAANLRKQELIFAILQAQTE
                     KNGVVFGEGVLETLPDGFGFLRAPDSNYLPGPDDIYISPSQIRRFNLRTGDIVSGQIR
                     PPKESERYFALLKVEKVNYEDPEVARDKILFDNLTPLYPEERLNLEFDREEYCTRVMD
                     LTTPIGKGQRGLIVAAPRTGKTMLLQAIARAILKNHKEVTLIVLLIDERPEEVTDWQR
                     QVKAEVISSTFDEPAQRHAQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNTIAP
                     PSGKVLSGGLDSNALQRPKRFFGAARNIENGGSLTIMATALIDTGSRMDDVIFEEFKG
                     TGNMEVHLDRRLADKRLFPAIDISQSGTRKEELLVDRDRLNKMWILRKVLSPLGTMEA
                     MEFLMDKIGGTKTNQEFLQSMNR"
     gene            12345..12758
                     /locus_tag="PLS35_11855"
     CDS             12345..12758
                     /locus_tag="PLS35_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNI19408.1"
                     /translation="MAKSDHDTPDRIFTLAEANELLPQLNQRFATIRRAKSVIANTKE
                     EITKASSKATSGGGSPVGALYIRALHEISGSLQAIHELGVVIKDVDLGLCDFPCKLEG
                     RIVYLCWKLGEEEIRWWHEVSCGYKDRHPLDGTAI"
     gene            12770..13057
                     /locus_tag="PLS35_11860"
     CDS             12770..13057
                     /locus_tag="PLS35_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNI19409.1"
                     /translation="MKFVILGFDGPEGQAKRKIHRAAHLAKLEPLNRQGRVFLAGPFT
                     DLAGSLIIIEADSLDEAKQIAQEDPYTVHGIFERVEVHPFKQVLPDTPSPS"
BASE COUNT         2913 a         4148 c         3384 g         2957 t
ORIGIN      
        1 gccacatgtt ggcccacgat ttctgcgatg tccactcggt ctctgatttg gttgatgacg
       61 tcgtccgaaa tcaggccttg gcccacggcg gtcctcgcta gtgcccgcag ggatcctacc
      121 ggtgaatgta ccggctggat gcctttgacg cgattgaaag ttgaccgttc aacctaacaa
      181 acggtccgca tatgtgtcaa tcaaggagca gtgcccatta tcccggaggc caggccatat
      241 gccttccgcc gaggacatgc aggtggagat gaaacaccgt ttgtccgctg tccgctccgg
      301 tgttcgtgac aatgcggtag ccagactcag ccaggttctt cattgctgca accttggagc
      361 aggtcatcag caaatgcccc aataacgcct gatcaccgtc ctgacaattc tggactgccg
      421 ccagatgacg tttcgggatg accaggatat ggacgggagc ttgggcattg acgtcctcga
      481 atgccaacac acgatcatcc tcgtggacga tttttgccgg aatggcccgg ttcacgatac
      541 gacagaagat gcagttatcc acgcgactcc ccttccggtg cctgacgaat cccggacttg
      601 ccgaacctgg ctccgagctc tcgatacact tcgctcagcg tcacatcatg gtaaccgaga
      661 accaggaggg tgtgaaaaaa cagatcggcc gtctcgtaaa taatctcgtc ccgtttctgg
      721 tttttcgcgg caatgatgac ctcgcccgcc tcttcagcca cctttttaag gatgcgatcc
      781 acattgcctt gcagcagctt ggacacatac gagtccggct gcggagcggc cttccgtgcc
      841 agaatcgtct gatagagccg ttcgagaatc cctccgccgg catcctgcgt cttgcctggt
      901 tcggcccgtc catctgcact cattctcgaa aaaaagcagg cgcgttcgcc cgtatgacag
      961 gtcggcccga ccggctccgc tttgacgaga atggtatccc ggtcacaatc gacgaacaga
     1021 tctttcaccc gcaaaaaatg tcctgacgtc tcaccttttt cccacaattt ctgacgcgag
     1081 cggctccaaa aatgaactga tttggtcgcc agcgtcttcg tgagggcttc ctgattcatg
     1141 tagcccacca tgagcacggt gccatcgaac cagtcttgaa tcacggcggg gattaaccct
     1201 tgcccgtcaa acgtcatact gcggctgtcg ctcgacatta cctgctccat ttaggcggcg
     1261 cctattcgaa ctggaacgcc acggtcccgc aaaaaggcct tggcctgctg aatagtatgg
     1321 gtccggtagt gaaaaatcga cgcagcgagc accgcctcgg ccttcccttt gaccaacccg
     1381 tcatagagat gctccaacgt acctaccccg cccgaggcaa tgaccggaat cgacagactc
     1441 tccgacacag cggccgtcag tgccagatca tatccgtttt gccggccatc ctggtccatg
     1501 ctggtcaaca ggatttctcc agctccatac tctgccatgc gctgagccca ttcgaccgca
     1561 tcaagcccgg tcgcgtttcg tccgccatga gtatacacct gccagcggcc tgcggcctcc
     1621 gctcgcttgg catcgatggc caccacaata cattgcgtgc cgaaacgctg agccgcctca
     1681 cgaacaaatt caggtttttg aaccgcggtc gtattgatac tgaccttatc cgccccggca
     1741 ttcagtaatg tgcggatatc atccagggtg cgcacgccgc ccccgaccgt cagtggcata
     1801 aacactcggg cggccgttcg ttccaccaca tcgacgatcg tctttcgatt ttcgtgtgaa
     1861 gccgtgatat ccaggaagca cagctcatcg gcgccttcac gatcatagat ggtggcgacc
     1921 tcgaccggat cgcccgcatc gcgcaaattg acgaagctga cgcccttgac gactcgaccg
     1981 tccttcacgt ccaaacaggg aatgatacgt ttggtcaaca taggaattcg cgacgcctga
     2041 aacgtgaaac ctgaaacgaa acggttctac tcctgcccgc caaacgagac ctcaacgatt
     2101 tccgagagcg gcgagggcag ctcgataatc caacttgccg tcatacaaag ctttgccgat
     2161 aattgcgcct tccacacgag ggccgagcgc gcggaccgct tgcagatcct ccacccgcgt
     2221 gatgccgccg gaggcaataa cgggaaaagc cgcacagttg acgacctccc ctaacgccgc
     2281 aaggttcggc ccattcaaca taccgtcgcg cgcaatgtcg gtatagatca ccgcacccag
     2341 ctcatggtcg gccagttcac ggaggagatc aatcgctttg gtgtcggaaa ccgcagtcca
     2401 tcccttcacg gcgaccttgc cgtctcgcgc atcaaggccc agcaggatac gccggggaaa
     2461 ttctcgacag gcttgcgcca ggaaggcccg atccgtcagg gcagccgtcc caaggacgac
     2521 ccgcgcgact cctgcctgga gatatttccg caccgtgtcg atcgttcgga taccgccccc
     2581 aacctgcacc tttacgttga cggccttcat cgctgactca atctgaggaa ggttctttgg
     2641 ttccccatca acagcgccgt tgaggtcgac gatatggatg agatcggccc cctgttcctg
     2701 ccaacgtttt gcgactgacg gcacatcctc cgaatacacg gtttcggccg ccatatcacc
     2761 ctgccgcagt cgcacacagc ggccatcttt cagatcgatc gccggaatta cacgcatatc
     2821 cgcacctgct tcctcacggt tacttccctt cacccgctgc tgcgccgaac ggaaacgcct
     2881 ccgctaattc ggtcgcccca taggccgcta actcttccgc ggtcacgaag gtcccatagc
     2941 gctgaacaaa cccaagcaca tcttccgtca tcggccgctg cttttcgtag tagttgcctt
     3001 cccataggac catgccatca ggggccacga gtttcacttc aaaccccacg gcagcgggag
     3061 ggtcggcacc caatcggctg ccgacacgtt cttgatagac caacactttc ccgctgagcg
     3121 cggcatcggc cttgagacgc tgcgcaattt tgtgagccgg aatggtctcc acagagccgc
     3181 ttcctctcga aagatcccga gccgcgccca tcgcttcctg aggggaaatg acctggagac
     3241 ccggcttgcc cttcaatttg gcccacatca gatctgtaat tttttcgccc gcaccgggcg
     3301 gcaccaaatt tgttcgccga accgcctgat cggtcgtggg cggaaccccc accgccatgt
     3361 cagaacgtcg gactcccgac ggcacaggga aggatggccc gcttgtttcg atggcctgag
     3421 gcgtcgccat tgtttcaaag ggaatcagca cgatgttgcg aacctgatac tttccagctt
     3481 gaggagaggc cttggaggtg accttggttc cgctgcaccc tgtggtcagc actacggaga
     3541 gcagaatgaa cagcgttcgc gggatgcagg tactcacgtc caggccccga aattcttaat
     3601 cacctgaaga ccgacggcct gactcttctc aggatggaac tggcaggcga cgacattgtc
     3661 tttccacacc gcggatgcga atggcgctcc atagtcagtc acggtggcaa cgaccgcgga
     3721 atcggcaggc tcaacataat aggagtgcac gaagtaacag taactgcccg gtgcaatccc
     3781 ggcaaatatt ggcgcaggcc gcatgatccg gatatcgttc cagcccatat gcggcacctt
     3841 taacgaagca tccagcacaa acctcttcac acggccggga atgattccca ggcccttgtg
     3901 tgttccgaat tcttcacttt ccgcgaagag tagttgtaaa ccgaggcaga tacccaaaaa
     3961 cggtttgccg gattgaatcg cggcatggat cggcccgatc aactcatatc gctccaggtt
     4021 cgccatgcaa tctccgaatg cccccacgcc gggcaacacc acatggctgg catccgcaat
     4081 gacgcgagcc tcgcgcgtaa ccacagcctg atgtcccacg gcctcaaagg ctttcgagac
     4141 actgcgcagg ttccccatac cgtaatcaat gatggcgatc atgttgcttt cgcggacgat
     4201 gcgtaatggc agctaccact gcccccgtct cacaccccgg atctgactag aggcttccct
     4261 tcgtcgagag aaccgctcct gacagccgct cttcgggcaa cgtggcttgg tccagcgccc
     4321 gcgccaacgc cttgaagatg gcttccatga tgtggtgagg attgcgacca tattgcaggt
     4381 ttatgtgcag attcaggccg ccatgggtga cgaaggcctg gaaaaaatcc tcgaacagcc
     4441 ccaggtcgaa cgttttgatc ttccggtccg gcaaattcac gttatagacc agatagggcc
     4501 gaccgctgag atccaccgtc acctgcgcaa gcgtctcgtc cagtggcact gacgcccatc
     4561 cgaatcgctt gatccccgcc ttttcgccta gcgcttgatg cagcgccttg cccatgacaa
     4621 tgcccacatc ctccaccgta tgatgctcgt cgatgtcgat gtcacctttg gcctgcacgg
     4681 tgagatcgaa aaacccatgt ttggctagca gatccaacat gtgatcaaaa aaccgaatgg
     4741 acgtgtcgat cttcccctgg cctgtaccgt ccaaggtcca ttcgacacga atatcggttt
     4801 cctttgtcgc gcgatgcaac gaggcctgtc tgggaactcc ggtgtttttc atgaaaacct
     4861 gctttgagct gatttggcat gcgcatcgaa gccttcgata tgcgccagtc tgacgatatg
     4921 gtccttggct ttcgtcaatt cttccctggt gtaatggacg atgttactga ccttcacata
     4981 gtcgttgacg gacaacggcg aaaagaaccg ggcagtggcg cccgtcggca gcacgtggtt
     5041 cggcccggcc acgtaatcgg ccacggcagg cggcgtataa cgacccagaa agagcgcccc
     5101 ggcatggcga atctgctcca gataatcaaa cggacgatcg accgataagg tcagatgttc
     5161 gggcgcgata tcgttcgcca cctcgatggc ctcatccatg gtaggaacga cgaacgcgac
     5221 aaaatgtcgt gcgatcgatt tggccgcgat tttctcccgc tgcaagccct tcaactggac
     5281 ctgaatgagc cgtacaacct cttttgccag cgatgcggag gtggtcacca ggtagacctg
     5341 cgcgtcttcg tcatgttccg cttcacacaa cagatcggct gcgacatgcg ccggcttcgc
     5401 atcgtcgtcc gccaccacca atagctcgct gggaccggcc accatatcga ttccgacggt
     5461 gccatagagc aatcgcttcg ccgtcgcgac atacatgttg cccggcccaa caatcttatc
     5521 cactttggca atgctcttcg tcccaaaggc catcgcgccg atggcctgca ctccgccgac
     5581 ccgatagatt tcatccaccc cggcgatatc agccgcgacg agcaaatagg ggttgatctc
     5641 gcccttcggc gtgggtgtgc acatgacgat gcgtcggact ccggccacct tcgcaggaat
     5701 cgcgcacatc agaaccgagg acggatagac ggctttgcct cccggcacat acacaccgac
     5761 ggcatcaagc gacgtcacca tctggccgag cgtggcattc tcctcctggt acatccaggt
     5821 tttggtgcgc tgccgttcgt gaaactgccg cacccgctca gcggcatacc ggagcgaatc
     5881 gccctcatct ttgcgaatgt gataataggc ttccttgatc tcctccggtg tcacacggat
     5941 ctgatcaagc gtgaccgaca cccgatcgaa cttcttggta taccgcagca ccgcgcggtc
     6001 tccgcctttc tccaccgcct gaaggatcgc gcgcacgctt ttctcgacag tggccgcttg
     6061 cgaacgagag cgggctgtga tttttcgtaa ggctttggcg aaggctcgat cggtactctc
     6121 gagaatagtc atgacatccg tcctgccgct gcgccgccag tatcgggcgc cgcatcatgg
     6181 gaacccacga ctgcccgcgt gggataggtc ggccgcgcta ctccgcagcc acggctgtgc
     6241 gcagccgatc gatcaacacc tgcaacggtt catgtttcaa tttgaggctg gcccgattga
     6301 cgatcaatcg ggcggtggag tgggtaatca cctctacctc cgccagatca tgggccttta
     6361 acgtactgcc cgtctcaacc agatcgacaa tgcgatcggc caatccaaca atcggggcaa
     6421 gctcgatcga cccatacagt ttgacgatct caaccggcat tccgcgctga ttgaagtagc
     6481 gctcggtgac gttggggtac tttgtggcca cccggacctt cgaggaccac cgatccggtg
     6541 atggctgtcc ctgcaacgcg gcgaccgaga ttctacacgc tcccaccttt aaatccaacg
     6601 gctcgtagac atcgctgccc tgctccagca agacatcttt ccctaccaca cccacatccg
     6661 cggccccatg ttcgacatag gtcgggacat cggtcggccg cacgatcaga aaggtggcat
     6721 tgttggcggc acagtcaaat accaacttcc ggctttccac agacaaccct gggctgtaat
     6781 aaccggccgc cttcatgaga tggagaaccg gcccgagcaa tttccctttc gacagcgcga
     6841 tcgtgagtcc tttcggcttt tcgtcttgcg atttcctcgc cttcacgttc actttccctt
     6901 tctccgctcg atgctggccc ccaagatccg gagcttttct tccaaccgtt catagccacg
     6961 atcgaggtga tacacacgcg acacctcggt ggtaccttcc gccgcgagac cagcgacgat
     7021 gaggcccgcg ctggcgcgaa gatcggaagc catcaccggc gcgcctgtca aacgttcctt
     7081 gcccgtaatc accactcgat tgccttcgac acgaatatcc gctcccatac ggcgcagctc
     7141 ttccacatgc atgaaccggc tctcgaaaat ggtttccgta accacgctgg ttccttcggc
     7201 caccgccatg agggccacaa actgggcctg catatcggtg gggaatccgg gatgtggact
     7261 cgttcgcaca ttcgtccctt tcaatcgccc cgtgaccttg agatgaacgc gttccttttc
     7321 ctcaatcaac tcggcgccgc tttcccgcag tttcatcaat aagggttcca tgtgctcggg
     7381 acggcaccga tcgacgatca cctcaccacc ggtaatcgct ccagccacca gataggtccc
     7441 ggcttcaatg cggtcgggaa tgacttcatg atcggcgcca tgcaattcgg tcacgccctc
     7501 aatcgtaatc atatctgtgc ctgcaccggc gatgcgcgca ccgcgcttga cgaggaatgc
     7561 cgccagatcg ctgatttcag gctctttcgc agcattttcc agtaccgtgg tgccctctgc
     7621 caacacggcc gccatcatga gattctcggt cccggtgacg ctgggcgtat caaagtaaat
     7681 gcgactgcct cgcaagcgac gcgcggtcgc cttgatgtat ccatgttcga tttcgaccgt
     7741 ggcgcccatt ttctccaacc cggccaagtg aaagttcacc ggtcgagagc cgattgcgca
     7801 cccgccggga agggaaacct tggcctcccc caatcgagcc acgagcggac ctaagaccag
     7861 caccgatgcc cgcatggtgc ggaccaaatc gtagggcgct tccgtcgatg caatggcttc
     7921 ggcctgcaca accgtctgat cgccctcctg agagacggca atgcccagca tgcgcagcaa
     7981 cttgcccatg gtcaacacat ccactactcg tggcatgttc gacagcacac attcgccccc
     8041 acccagaatg gtggaagcaa gaatcggcaa tgccgcgttt tttgccccgc tggtgcggac
     8101 ctcgccttgg agtcgacgtc cgccttgaat aagaatctga tccatcagtc tcagggaatc
     8161 attggtgcat catcgatggc tatggaggtc aggacgatga agatgataag gactcttctt
     8221 ttttctcgaa acagacgacc cgttcaatgc cgccctgatc ccgcaacacc acataggtac
     8281 gaagccgacc accttcttca gcatgacggc agacagctgc tgcctggccg attcccactt
     8341 ccataagcag ggccccacct ggcttcaaat acgccggcgc ctgtgcaatc aagcggcgat
     8401 gcacatccat gccgtcatga cctcctgcca gcgccaaccg cggctcgaaa tctcttacct
     8461 ccggctgaag ggtctcgaca tccccgtccg ggatataggg agggttggac aggatgactt
     8521 caacctgatt gggcaaccct tcctgaccga tcggatccag caagtccccc tggaggcaag
     8581 taatacgtgc ctcaaccccg taccgtgcca tgttgtttcg tgcgaccact aaggcatcgg
     8641 tcgaggcgtc gatggctaat acgcgcgccg tcggcaaagc cgaggccacg gatacggcca
     8701 aacaccctga tccggtcccc acatctacga tggtcgcgtc tgatatctgc acgcaccgac
     8761 ggactgcttc ctccaccaac aatacgctct caggacgggg aatgaggacc gagggggtga
     8821 cgaaaaaatc ccgtccgcaa aactcttgcg taccaagcac atactgcagc ggctcacgat
     8881 tggctcgccg ttcgaccaac acctgcactc cctcacattc ttcaggcgtt aaaccgcgct
     8941 ctgtattgat tcgctgcatg agtggcgaga gtttcaaaac atgctccacc aaccaggcgg
     9001 attccaaggc gggctggtcg atgcctgcgg catccaggcg agattccgac tttctcagta
     9061 actcgccgag actcgatacg gaacaggcgg cgtctgccaa ctgctgctgc ctcattttgt
     9121 ttccagactg gccaaatgtt cgtaatgcgc tctcaacgcc tgcacaattt cctccagatc
     9181 gcccgccaac acctgatcaa gcttatgaac ggttagtcca acgcggtggt cggtcacacg
     9241 gttttggggg aaattgtacg tgcggatttt ctcgctccgg tcaccggtcc caacctgcga
     9301 cttacggtct tgggcaatag ccgcctcctg cttttcccgc tctgcctcca caatccgcgc
     9361 ccgcaaggtt cgcatggctt tggttcgatt tttcagttgt gaccgctcat cctgacagct
     9421 gacgacgacg ccagtgggaa tgtgcgtgat gcgaacggcc gagtacgtcg tattgacgct
     9481 ctgtccacca gcacccgagg aacaaaatgt atcgatacgc agatctttgg gatcaatgtg
     9541 gacatccact tcgtccacct ccggcatcac cgccaccgta accgtagagg tgtgtattct
     9601 tccactggct tctgtagtag ggacgcgctg cacgcggtgc actccgctct cataccgcag
     9661 acggccgtaa gctccctttc cctctagcag agcgatcact tcacggtagc cgccgatgcc
     9721 cgtctcagag gcatcgacca cctccagccg aaggcgattc cgctctgcat acttgagata
     9781 catgcgaaag agatctcccg caaataaggc ggcctcgtct ccccccgtgc cagcccggat
     9841 ctcgagaaac acattcttct catcgcgggg atctttcgga gtaaggaagt ccttcgcgcg
     9901 agcctcgatg tccaagagct gttgagtcaa atgctcgctc tcttctgcaa agagctgctt
     9961 catgtcagcc ccggtgccgg aatcctgcag catcaccgtc acttcgtgga gttgcttctg
    10021 ggcttgggaa taggcggcaa actcctgagc aggctcctct aactccgttc gctctttatt
    10081 gagcttgtga atcatgcccg gctgattgag gaccgtcgga tccatgagct gattcgtcag
    10141 ctcctggaac cgcgcagcca tcgcttccca ttttttgatc agcccatctt ccatgtctcg
    10201 cccattccgg ccatagccct gaagcccaaa aaacaagaga ccctgcccca aaaggaagca
    10261 gggtctccat gatgtcctga ctgaggccta cttgtctttt ttggcgtact tctttttaaa
    10321 ccgttcgaca cgcccttccg tatcgacgat cttctgggtt ccggtaaaga acggatggca
    10381 ccctgcgcaa atgtcgacct taatgtcacc aatagtggat cgggtcttga acgtgttccc
    10441 gcaagcacag tgcactgtcg cttcacggta gactggatga attccctttt gcatacctgc
    10501 tcctctactg acctagaatc attgactcgt cctgctcgcc agggtgcacg actttacact
    10561 ggagtggcct acactggcaa gcggtgccta ccgattcatt gattgcaaaa attcctgatt
    10621 cgttttggtc ccaccgatct tgtccatgag gaactccatg gcttccatgg tgcccaacgg
    10681 gctcaagacc ttgcgcagaa tccacatctt gtttaagcgg tcacggtcaa ccagcaactc
    10741 ttccttacga gtgccagact gactgatatc gatggcaggg aagagccgct tgtccgccag
    10801 gcgccgatcc aggtgcactt ccatattgcc ggtacccttg aattcttcga agatcacatc
    10861 atccatgcga ctaccggtgt cgatcagcgc agtggccatg atggtcagac tgccgccatt
    10921 ttcaatgttc cgagctgcac cgaaaaaccg cttcggccgc tggagagcat tagagtcaag
    10981 accgcccgag aggaccttcc cgctgggagg ggcaatcgtg ttgtaggccc tagccaaccg
    11041 agtgatgcta tcgagcagaa tgaccacgtc ttttttgtgc tcaaccagac gcttcgcttt
    11101 ttccaatacc atctccgcaa cctgcgcatg ccgctgagcc ggctcgtcaa acgtggagct
    11161 gatgacttca gctttgacct gacgctgcca atccgtgact tcttccgggc gctcgtcgat
    11221 caggagaaca atgagggtaa cttccttgtg attcttcaga atggctcggg cgatcgcctg
    11281 cagcaacatc gtttttccgg ttcgcggagc tgcgacaatc aaaccgcgct gccccttacc
    11341 gatcggcgtc gtaaggtcca ttacacgcgt gcaatattct tcccggtcga actccagatt
    11401 taaacgttcc tcgggataga gcggggtgag attgtcgaac agaatcttgt cacgcgcgac
    11461 ttccgggtcc tcataattaa ctttttccac cttgagcaac gcgaaatacc gttcgctctc
    11521 tttcggtggg cgaatctgcc ccgatacgat atctccagtt ctcagattga agcggcggat
    11581 ctgcgatggg gagatataaa tatcgtcagg tcctggcaaa tagttcgaat ccggcgctcg
    11641 aaggaaccca aagccgtccg gcaatgtctc caagaccccc tcgccaaaca ccacgccgtt
    11701 cttttcggtc tgcgcctgca gaatggcaaa aatgagttct tgcttccgaa ggtttgccgc
    11761 gccttcaatc tttaaatccc gggccacgtc attcaggtcg gcaatcgact tctgtttgag
    11821 ttccgcgaga tgcataactt cccctttaac ctgtgtcata cctcttccct atcgggctct
    11881 caacccgata gccccccaaa atgagaaaaa cgatttagta ctcatgaacg aatttcacgg
    11941 aagtgatttg tctgggtatc aacctgttgg ttgttttatg gcaggatcgc tttgaaggcg
    12001 ttgatgattg aaaacttgca gaaatgaaag caaccccctg ttgcacactc gatgatcaac
    12061 tacctgggat ttttacgatt ggaatgggtt cggaagctga tgcacagaag gtaatgcgtt
    12121 tttcagaatc gcgaagttat tgtcattcta gcgtccaacc ttctcattgt caacaaatta
    12181 aaccccctga gaacccctgc acgtgaaaca gatcgacgca ccaccgcatc atttgctagt
    12241 gacaagctgg gaacttcata ggagctcaac cgcacgcgag ccgatcaggg accttggttg
    12301 caggccaaca accattatgt taaagatcgc gcgaggaatt ctttgtggca aaatcagatc
    12361 acgatacccc agatcgcatt ttcaccctgg cagaggctaa cgaactgctt ccccaactca
    12421 atcaacggtt tgccactata cggcgggcta aatccgtcat cgcgaacacc aaagaagaga
    12481 tcaccaaagc cagctctaag gccacgtcgg ggggcggcag ccctgtcggc gcactgtata
    12541 ttcgggccct ccatgaaatc agcggcagct tgcaggcaat tcacgagcta ggagtggtga
    12601 tcaaggatgt tgacctagga ctttgcgact ttccctgtaa acttgaaggc cgcatcgtat
    12661 acttgtgttg gaaattgggc gaagaagaaa ttcggtggtg gcatgaagta tcgtgtggtt
    12721 ataaggatcg ccacccattg gatggcactg ccatatagca ggactctcaa tgaaatttgt
    12781 cattttaggg tttgacgggc ctgaggggca ggccaagcga aaaatccaca gagctgccca
    12841 tctggccaag ctggaacctc tgaaccggca gggaagagtc ttccttgctg gtcccttcac
    12901 tgacctggct ggcagcctga ttatcattga ggcggactcc ctcgacgaag cgaagcagat
    12961 cgcacaagaa gatccttata ccgttcacgg aattttcgag cgtgtagaag tccacccttt
    13021 caagcaagta ctccctgaca ccccttctcc ttcctaggat accctggcat ccagacacgg
    13081 atctctcatt ccggtgagcc gtcaaactgg tcgctgccgt agcgagaagc aagcaatagc
    13141 ttcaagccct gccagattga caagtaaaaa gattgccacc tataatgcgc acttagagct
    13201 atttgggaag atgtgtgcct tgtagttcaa aagacctaag tgccttctca cgcgctggcc
    13261 ctaaatttcc cccaggtggg ccttctgctg tgttgcagaa aaacatcgag aaagcataga
    13321 gtgacttgac cccacgcttt aaccgagggt gtcccttctc caagtttgtt aaccccaaaa
    13381 agagagcagg ttcgaatgac tc
//
LOCUS       DAOQEW010000104        13376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1332_length_13376_cov_8.519180, whole genome shotgun sequence.
ACCESSION   DAOQEW010000104 DAOQEW010000000
VERSION     DAOQEW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_13376_cov_8.519180"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..484
                     /locus_tag="PLS35_11865"
     CDS             197..484
                     /locus_tag="PLS35_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19410.1"
                     /translation="MERAKPKLLNRVSNSRPKRTTSPLPSHIRDEISSILADALLADL
                     QADSRPMAKTHAKYGQKWPLTDNHQSAMTLAGLELQSLEFEKNEDVSEPKE"
     gene            489..2123
                     /locus_tag="PLS35_11870"
     CDS             489..2123
                     /locus_tag="PLS35_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNI19411.1"
                     /translation="MIAAIYARKSTEQSGVIEEEKSVTRQIEHAKAYAASKGWNVSEE
                     HIFVDDGVSGAEFVKRPGFIRLMNALKPRPTFHLLIMSEESRLGRESIETSYALKQIM
                     DAGIRVFFYLEDRERTLDNALDKVLLSLTNFASEMERERAKQRTYDAMLRKAKAGHVT
                     GGKVFGYDNREILSAEGRRLHVLRVINAAEAAVVRQIFEMFAGGVGIGRITKRLNEEC
                     TPAPRQSPRGWAPTAVREIIRRPLYKGEIVWNQHEKIVRGGTKKRRERNEKDWIRLDA
                     PDLRIISPELWDSVAKRLEKRKGQRRSAFRDVESKYLLTGMARCAHCGGPMTIVGQDY
                     HREKGRFYGCSYYKSRGSSICKNSLLVEQELLDQTVLKAIEEALTEEMLKVAIEKALT
                     KHRAGASVQLDHRSRIERELSLIEAQLKNLVDAVANGRKESALFERLANEEERKRSLI
                     ADLERLTRPEHVPHLDEARLKRELKARFADTRALLGRHITSARRLLRVLMERPLRLET
                     MQDGDRKGYRVTGTGSYLPLLSETRHSVESGVPNGI"
     gene            complement(2102..2179)
                     /locus_tag="PLS35_11875"
     tRNA            complement(2102..2179)
                     /locus_tag="PLS35_11875"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2141..2143),aa:Glu,seq:ttc)
     gene            2341..4308
                     /gene="glgB"
                     /locus_tag="PLS35_11880"
     CDS             2341..4308
                     /gene="glgB"
                     /locus_tag="PLS35_11880"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNI19412.1"
                     /translation="MDPVRSHSQNAPLGVVPSSAAPAHIGSLLSPEDLYLFNEGTHFH
                     LYDKLGAHPTTVQGVEGTYFAVWAPEAEQVSVFGTFNHWDPDRHPLRPCQSSGIWEGF
                     VPGVGIGALYKFHIRSRNHGAVLIKTDPFARLNEIPPKSASVVWDLNYTWQDHDWMQA
                     RARHNALQAPISVYEVHLGSWMRVPGEGNRSLSYREAAPKLIEYVQRLGFTHVEFLPL
                     MDHPFFGSWGYQTTGYFAPSGNYGTPQDLMYLIDQLHQHNIGVILDWVPSHFPTDEHG
                     LSRFDGSHLFEHADPRQGLHPDWGTAVFNYNRNEVRSFLISSALFWLEQYHADGLRVD
                     AVASMLYLDYSRKEGEWIPNKHGGRENLEAIAFLRQLNEEIYRRHPDVQTYAEESTSW
                     PSVSRPTYAGGLGFGMKWDMGWMHDTLEYMGLDPVYRKHHHRNLTFRMLYAFQENFLL
                     PLSHDEVVHGKGSLLGKMPGDDWQKFANLRTLFGYMYAQAAKKLIFMGGEIGQWREWA
                     HDDSIDWNLLNYPPHQGLQRWVADLNHLYRAEPALHEFDFDPRGFEWIDCQDVDAGTI
                     SLLRHGRSSRESIAVVCNFTPVPRLQYRVGVPQGGYWKELLNSDASLYGGSGLGNLGG
                     LSADAVPAHGRSHSLALTLPPLAVLFLKSAS"
     gene            4452..4895
                     /locus_tag="PLS35_11885"
     CDS             4452..4895
                     /locus_tag="PLS35_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19413.1"
                     /translation="MLVIALSLAHCSHDIHEKRADTIKGHVESFYDHLKHDRVAAAVR
                     ENEAIEQLSTQLGEIVTRRVNQPGTNQIDREWTDLRTANETAAQNWLALGQYLSIKKQ
                     YAQSRATYQRVIDTYNGATERTYREQAARAIRDLDILSPPPAAPR"
     gene            4921..5472
                     /locus_tag="PLS35_11890"
     CDS             4921..5472
                     /locus_tag="PLS35_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19414.1"
                     /translation="MRFIASRLMILVMGLCLLLTGCVHRIHVNPASNSPATITIPAAV
                     QLEVPFLAIEGADHMPGIAMLDWPAKDLRQAIVRYFSERQTFRAIGTDPADLKMVVKA
                     WLTLRAPDRYLYRVHLEADLSHPGHGVFKTYAAEGEALGSSVRWITASDQEPIDEATS
                     QALQQLATQLEQDRDLIMNNFSR"
     gene            5913..7088
                     /locus_tag="PLS35_11895"
     CDS             5913..7088
                     /locus_tag="PLS35_11895"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HNI19415.1"
                     /translation="MILCFDGEWDCPPDPGVWSFSANHSPKQSRTGVQPDSHSATNVY
                     QLYQSILPRSGDGRIQQKWYEPGTTMPWFHRFRDGSFGCGLDRTILQSYAYLAATYDP
                     GDELYLYGFSRGAYTARSLVGLLTLTGLPAPVLLNADHVRLVREAASPLSPSGGVAAH
                     LRECLTRMVTDPDNHVIDEAYRVYRNLQNASQIATGRRKGSSDINVTLLGLWDTVGPL
                     GIPTKALRWLNEHRYNFRDTELSPIVRHACHALAIDEHRADHNATLWTSPPKAAQVIE
                     QRWFAGAHGDLGGTYPDRDLADVPLAWIRQHSMAKGLALESGRIDEKLNMLGPIHDSF
                     SECFVGFRRWIHPRFYRPVMQTGTGTEVLDESVHTRLLHSPAYRPKNEGLNNLLTFR"
     gene            7313..7675
                     /locus_tag="PLS35_11900"
     CDS             7313..7675
                     /locus_tag="PLS35_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19416.1"
                     /translation="MKRSSQTKLLVHCILSDVAMGCAEEGKVFDVGQRGTLRTTFHNL
                     IDGDHLSVKIQLRDERRPVSVHLAKVSWIQGERFGVELLVMDVDERLRLNRFLEAKLP
                     LELEFQDSHTELTIRAAD"
     gene            8099..8278
                     /locus_tag="PLS35_11905"
     CDS             8099..8278
                     /locus_tag="PLS35_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19417.1"
                     /translation="MSIDVELALVLGLFMIGAVVLAIDNLVYALSKRFSRWMRNRKSV
                     PRTPSTFTSTQKKQF"
     gene            complement(9033..10463)
                     /locus_tag="PLS35_11910"
     CDS             complement(9033..10463)
                     /locus_tag="PLS35_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNI19418.1"
                     /translation="MRSLALVLFSTLLAACAVGPDFNRPEATTPDAFRMAEPGGDAAS
                     IANTPWWELLKDQELQKLIRTALEENKDLKRAAAAIEEFQARLFIAKTDYAPQMNVTA
                     NAPLFGRKSNFLFPGFPNPFNYYLQGNLSWEIDIWGRIRRSNEAARGDLLSREENRRA
                     IVLQLVSGVAEAYFDLLQFDMQLDIARRTLKSWEESVRIAQARLRQGMISKLDADQFE
                     AERANAAAKAAEFERQKVQKENQLSVLLGRNPGRIARGYSLTEQVMPPDVPPGLPSEL
                     LQRRPDILQAEQDLAAATARIGMAKADRFPKLSITGILGVASPHLSRLVANETAFGVA
                     GPGLAGPLLNAQILGFQQRAAEAQARQAVAQYEQSVLVAFKEVEDSLVAVRTVREQRA
                     AQLQQVEALRSALSLANLRYKGGLANYLDVLIAQRNLFEAELALMGTHRLHLVSIVQL
                     YKALGGGWTPEGQLPVSPIASPPEKS"
     gene            complement(10456..>13376)
                     /locus_tag="PLS35_11915"
     CDS             complement(10456..>13376)
                     /locus_tag="PLS35_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNI19419.1"
                     /translation="MSIKLTFEIGTDVDIAQVQTQNRVKLAEPQLPPEVVRQGISINK
                     VSPDLLAVVALSSTDPTHDTVYLSNYAILRVLDNLKRLRGVGNAVVFGSQNYSMRLIL
                     DPIRMAQLSLTPTDVANVVREQNRDFPAGTIGREPALKGTELTIPVITQGRLTEVKDF
                     EDMIVRAMPNGSMVRLKDVARVELGAQSYTLEGRWNGKPNVFLLTFLSPGANALETVK
                     RVRAELTEVSKAFPTGVSYDIPYDTTRFIDVSIKEVVKTLAEAMVLVILVVYLFLQSW
                     RATLIPGVAVPVSLIGTFAGMQALGFSINTLTLFGMVLAIGIVVDDAIVVVENCERHM
                     TQGKLSAKNAAKRAMEEVTGPVIAIVLVLCAVFVPVGFLGGITGELYKQFAITISIAV
                     IISGFVALTLSPALCALVLKPGEERHHGLFGLFNRTFSWMQGRYISTVGMALTRRLLS
                     VAVFAVLLTGVVLLFKTIPNSFLPEEDQGYFITIVQLPDGASKQRTDTVLSKIENYFL
                     ANPAIHSTDALSGQNFVFSTRGPNAATMFVPLKHWDERTAPQQHVKALIGAAYGEFAK
                     IPEALILAFNAPSIRGLGATGGFSIQLQDPSGGDFNKFSAVAQEFVAKARQNPAIGAI
                     GTSFRVSAPRIFAKVNRERAKALGVPISEVFDTLQAYFGNLYINDFVKFGRVFRVQTE
                     AEAQYRSTPDDISKIYVRAVGAQGPTMIPLDTVVSTEFSSGPDPVTHFNGYNTALVLG
                     SAAPGVSSGQVLDALDKLGKEVLVPKGYGIDWSGISYQERMVGNQSLYAFGFGLLMVF
                     LVLAAQYESWVVPFAVILAVPIGLFGALSAVWLKGMTNDIYFQIGLVTLIGLSAKNAI
                     LIVEFANKRYEEGHPLLEGTIDAAKLRFRPIVMTSMAFILGVVPLVVATGAGAASRNS
                     IGTGVFGGMLAATFLAIFFVPLFFVLIRSLSRGRNRQLPPPGADGPDQPEKERDYQHA
                     "
BASE COUNT         3049 a         3920 c         3577 g         2830 t
ORIGIN      
        1 ggcacggggc tctcgcagcg ggcaggcctg gaggggcgga gaccggcacg gaggggaccg
       61 ggaaggcctg ctccgctgct ccccggcccc gggccgtatc cactgggggc tcactcttcg
      121 acccaacctg cggaagacgg ggcgcttccg cgtagtgaga gaggggcttt ggatcacttg
      181 aggagaagct attcagatgg aacgggcaaa acctaagcta ctcaacagag tgagtaattc
      241 tcggcccaaa aggaccacct ctcccctccc gagtcacatc cgagatgaaa tttcatcaat
      301 tcttgccgat gcgctgcttg cggatcttca agcagatagc agacctatgg ccaaaaccca
      361 tgcgaagtac ggacaaaaat ggccattgac tgataatcac cagagcgcta tgacgcttgc
      421 aggattggag ttacagtccc tggaattcga aaagaacgaa gacgtgtcgg aaccgaagga
      481 gtagcccagt gattgcagcc atatacgcac ggaagtccac tgaacaatct ggagtgattg
      541 aggaggagaa atccgtcacc cggcagattg aacacgcaaa ggcgtacgct gcgagcaagg
      601 ggtggaatgt ctctgaggag catatcttcg tggatgacgg tgtttccggg gcggagtttg
      661 tgaagcgacc ggggttcatt cggctcatga atgctctcaa acctcgtccc accttccatc
      721 tgctgatcat gtccgaggag tctcggctgg ggcgggaatc cattgaaaca tcttatgccc
      781 tgaagcaaat tatggacgca ggaatacgag tcttcttcta tctggaagat cgtgagcgta
      841 ccctcgacaa cgccctcgac aaggtgctgc tctcacttac taatttcgcg tctgaaatgg
      901 agcgtgagcg cgctaaacag aggacctatg acgcgatgct ccggaaagca aaggccggac
      961 atgtcactgg cgggaaggtc tttggatacg ataatcggga gattctttct gccgaggggc
     1021 gaagacttca tgtgcttcgc gtgatcaatg cggctgaggc cgccgttgtc cgccagatct
     1081 tcgagatgtt tgcaggcggc gtcgggatcg gccgtatcac caaacgactc aatgaggaat
     1141 gtactcccgc gccacgccag agtccgcggg gctgggcgcc cactgccgtg agagaaatta
     1201 tacgccgacc actgtacaag ggagaaattg tctggaacca acacgaaaaa atcgtgcgag
     1261 gaggtaccaa gaagcggcgg gaacgcaatg aaaaagattg gattcgctta gatgctcccg
     1321 atttgaggat catttctccc gaactgtggg actccgtggc aaaacggctg gagaaaagga
     1381 aagggcaacg acgctcggcc ttccgtgatg tcgagtcgaa gtatcttctc acgggaatgg
     1441 cacgctgcgc ccattgtggg ggacccatga cgatcgtagg gcaggattat caccgggaaa
     1501 agggacgctt ctatgggtgc agctattaca agtctcgcgg ctcgtcgatt tgtaagaatt
     1561 ccttgctcgt ggaacaagaa ctacttgacc aaacagtcct aaaggctatc gaggaggcgc
     1621 tgaccgagga aatgctaaaa gtcgccatcg aaaaggcatt gactaaacac agagccgggg
     1681 cgagcgtgca gcttgatcat cgctctagga ttgagcgcga attgtcccta attgaggctc
     1741 agttgaagaa tttggtcgac gccgtcgcta acggtcgaaa ggagagtgcg ctgtttgagc
     1801 ggctcgccaa cgaggaagag cgaaaacgct ccctgatcgc ggacctggaa cgactcacgc
     1861 ggcctgaaca tgtcccccat ctcgatgagg ctcgcctcaa acgtgaactg aaagcgcggt
     1921 tcgcagatac gcgagcgctc ctagggcgcc acattacttc tgccagacga ctgcttcggg
     1981 tcttgatgga acgcccatta cgcttggaga ccatgcagga tggggatcga aaggggtatc
     2041 gcgtgactgg gactggcagc tatttgccac tgctgtctga aacgaggcac tccgtggaaa
     2101 gtggtgtccc caacgggatt tgaacccgtg ttactgcctt gaaagggcaa tgtcctaggc
     2161 caggctagac gatggggacg aagaaagttc cgtatcggcg ggatgattca taccacactt
     2221 gatcgagagg tgtcactagt ctgaaccaag atcctcctga acttcatgcg gaccctgacc
     2281 gccattccgg ttcgaagcgt ccgattgacg ccctgcccct aaccacagta ctctgcgcct
     2341 atggatcctg tccggagtca ttcacagaac gcacccctgg gcgtagtccc ttcctcggca
     2401 gcgccagccc atatcggcag tctcctgtcc cctgaggatc tgtacctttt caacgaaggc
     2461 acacactttc atctgtatga caaactgggc gcgcatccaa cgactgtcca gggcgtcgaa
     2521 ggcacctatt ttgcggtgtg ggcgccggag gcggagcagg tctcggtgtt cggcaccttc
     2581 aaccattggg atccagaccg ccatccgctc cggccttgtc agtcatccgg catttgggag
     2641 gggtttgttc ccggcgtggg gatcggcgcc ctgtacaagt ttcatatccg gtcacgaaat
     2701 catggggccg tcctcatcaa gaccgaccca ttcgcccgcc tgaacgaaat tccgccgaaa
     2761 tccgcgtctg tggtgtggga tctgaactat acctggcagg atcacgactg gatgcaggcc
     2821 cgcgcccggc acaatgccct tcaggctccg atcagtgtgt atgaagttca tctgggctcg
     2881 tggatgcgcg ttcccggcga aggcaatcgc tcgctcagct atcgtgaggc tgcgccgaag
     2941 ttgatcgaat atgtgcagcg gctggggttc acgcacgtcg agtttctgcc cctcatggac
     3001 catccattct tcggttcctg gggctatcaa acgaccggct actttgcccc ttccggtaac
     3061 tacggcacgc cgcaagacct catgtacctc atcgaccagc tccaccagca taatattggc
     3121 gttatcctgg actgggtgcc ctcacacttc ccgaccgatg aacatgggct gagccggttc
     3181 gacggcagtc atctttttga gcatgccgat ccccgccagg gtctccaccc cgactgggga
     3241 acggcggtct tcaattacaa ccgcaacgaa gtacgcagtt ttctcatcag cagcgcgctc
     3301 ttttggctgg agcaatatca tgccgacggc cttcgggtgg atgccgtcgc ctcgatgttg
     3361 tatctcgact actcgcgcaa agagggggaa tggatcccca acaaacatgg cggccgtgag
     3421 aacttggagg ccattgcctt cctccgtcag ctcaatgagg aaatttatcg gcgacacccg
     3481 gatgtccaaa cctacgcaga ggaatcaacc tcatggccgt ccgtgtcgcg tccgacctat
     3541 gccggcgggc ttgggttcgg gatgaagtgg gatatgggct ggatgcacga tacgctggaa
     3601 tatatggggc tggatccggt ctatcgtaaa caccaccatc gcaacctgac cttccggatg
     3661 ctctacgcgt tccaggaaaa tttcttgctc ccgctttccc acgacgaagt cgtacacggc
     3721 aagggctcgc tcttggggaa aatgcccgga gacgattggc agaagttcgc caacttgcgc
     3781 acgctgttcg gctatatgta tgcgcaagcg gccaagaagc tcatttttat gggtggggag
     3841 atcggccaat ggcgcgaatg ggcgcatgac gacagtatcg attggaatct gctcaattat
     3901 ccgccgcacc aaggtttaca gcggtgggtc gccgatctca atcatctcta tcgcgctgaa
     3961 ccggccctgc acgagttcga ctttgacccc cgcggattcg aatggatcga ttgccaggat
     4021 gtcgacgcgg gtaccatcag ccttctccgc catggacggt cttcccgtga aagtatcgcc
     4081 gtcgtatgta atttcacgcc ggtcccgcgg ctgcagtatc gagtcggcgt gccacagggc
     4141 ggctattgga aagaattgct gaatagcgat gcctccctat acggcggcag cggactggga
     4201 aatctcggcg gtctctcggc cgacgcggtt cctgcccacg gtcgatccca ttcgctcgcg
     4261 ctaaccctgc cccccctcgc cgtcctgttc cttaagtctg cctcgtaaag ccgactacca
     4321 actcgccttt ctcctccggc tcaggtagga tcacggcagg catcaagcac caccgaacga
     4381 actgatccgt tcataaggga gggatgatgg cgatacgctc atgcagaacg ttgatctggc
     4441 aaacaccatt gatgctcgtg atagccctct ctcttgcgca ttgctcgcac gacatccatg
     4501 agaaacgcgc cgataccatc aaaggtcacg tcgaatcgtt ttacgatcat ttgaagcatg
     4561 atcgagtcgc tgccgctgtt cgtgaaaacg aagccatcga gcaactctcc actcagctgg
     4621 gagagatcgt gactcggcgt gtcaatcagc cgggcaccaa tcagatcgac cgtgaatgga
     4681 cggaccttcg caccgccaat gaaactgccg cgcaaaactg gctggctctg ggacagtatc
     4741 tttcgatcaa gaaacaatat gctcaatccc gggccaccta ccagcgcgtc atcgacacct
     4801 ataatggcgc aaccgaacga acctatcggg aacaggcggc gcgagccatt cgcgacttgg
     4861 atattctcag tcctcctcct gcggcccctc gataaccaga actaccgata aactcgaacc
     4921 atgcgattca ttgcatcacg gctgatgatt cttgtgatgg gcctctgcct gctcctgacg
     4981 ggatgtgtcc acagaatcca cgttaatcct gcctcgaaca gtcccgccac gataacgatt
     5041 cctgccgctg tgcaacttga ggtgccgttt ctcgccatcg aaggtgctga ccacatgccg
     5101 ggcattgcca tgctcgattg gcccgcaaaa gatctgcgcc aagccatcgt ccggtacttt
     5161 tctgaacggc agaccttcag agcgatcgga acagatcctg ctgacctcaa aatggtcgta
     5221 aaagcctggc tcacccttcg cgcaccggat cgttacctgt atcgagtcca cctggaggcc
     5281 gatctatccc atcccgggca cggagtcttt aagacctatg ctgccgaagg cgaggctttg
     5341 ggatcgtcag tccggtggat tacggcctca gaccaagagc ctattgatga agccacctct
     5401 caggcgctcc aacaattggc gacccaactc gaacaggatc gagacctcat catgaacaac
     5461 ttcagccggt agacaggaac tccacttgga agatctttca cggcctcacc atcgttgacg
     5521 tctcgtgctt ggccaggaat ccatcgaccc ggccgtaaga ccagctccat gcgagcttcg
     5581 agttgccgta tggtcgcggc acgcctgtcc tgactttccc gtatacgttt agatactgct
     5641 gcgtatcgca tcagatacga ccctccgcct cctcaatttc tacagagcac ctcgcgccac
     5701 ctgcattgcg cccaattgag gacctatgca tttctcgcta ccggaaattg caatgcgctc
     5761 gactggcatt agctttgctt attccgctgc ctgcggttca aaacctgtca ccactgacgt
     5821 ttgagtgctc ctgatgaacg gagtcggctc acgcgggagg cggtttcaca gcctgcggtt
     5881 gtcgtggaga aagtgcccgc gtgtccaaac gtgtgattct gtgtttcgat ggtgaatggg
     5941 actgtcctcc agatcccggc gtctggagtt tttcagcgaa ccattctccc aagcagtctc
     6001 gaactggcgt tcaacccgat tcgcattcgg ccacaaacgt ctaccaactc tatcaatcaa
     6061 ttctgcctcg ttcaggcgat gggcggatcc agcagaaatg gtacgaaccg gggacgacaa
     6121 tgccttggtt ccaccgcttc cgcgacggtt cgttcgggtg tggactggat cgaaccattc
     6181 ttcagagcta tgcctatctg gcggccacct atgatcccgg cgacgagttg tacctgtatg
     6241 gcttcagtcg aggcgcctat accgctcgct ctctggtagg attactcacg ctcaccggtc
     6301 tcccagctcc ggtgctgctc aatgcagacc acgtcagact tgtacgggaa gcggcaagtc
     6361 ctctctcacc ctcaggtggt gtcgccgccc atcttcgcga atgcctcact cgaatggtca
     6421 ccgatcctga caaccacgtc atcgacgagg cctatcgtgt gtatcgaaac ctgcagaacg
     6481 cctcccagat tgcgactggt cggcggaagg ggtcttcgga catcaacgtc acattgctcg
     6541 gtctttggga taccgtcgga ccgctgggta ttcccacgaa agcgctcaga tggctgaatg
     6601 aacaccggta caactttcgc gatactgaat tgagtccgat tgtgcggcac gcctgtcatg
     6661 ccctcgctat cgatgaacat cgagcagatc acaacgcaac actctggact tccccgccca
     6721 aagccgcgca ggtgattgaa cagcggtggt ttgccggagc acatggagac cttggcggaa
     6781 cgtatcctga tcgagatttg gctgacgtgc ctttagcctg gatacggcaa cattccatgg
     6841 ccaagggatt agctctggag tcaggcagaa tagacgaaaa acttaatatg ttaggcccca
     6901 ttcacgactc gttcagtgaa tgttttgtcg gatttcgaag gtggatccac ccgcgattct
     6961 atcggcctgt gatgcaaacg ggcacgggca cagaagtctt agatgaatca gttcacactc
     7021 ggctcctgca ctctcctgcc tacagaccga aaaatgaggg gctgaacaac cttcttacat
     7081 tccgctgact ggtgagctgt cgcccttaac gctcatccct ctacaattag tcgggacgcc
     7141 tccaaagttt aaacagcgcc tctgcaacgt cgcttcatcc ttccgttggc acctagccat
     7201 ctgcccccct ccatcgcagt accctgtttg cagactatct gatcagactt tcacgagata
     7261 ttctcgccat cgagcggtat gtggcatcac gcgcatacgc gggagggcaa ggatgaaacg
     7321 ttcatcgcaa acaaagcttc tcgttcactg cattttgtcg gatgtagcca tgggctgtgc
     7381 agaggaggga aaggtatttg atgtggggca gcgcggaact ctacgcacaa ccttccacaa
     7441 tctcatcgac ggggatcatc tctccgtgaa gattcaactc cgtgacgagc gacgaccggt
     7501 ctccgtccat ctggcaaaag tcagctggat acaaggagaa cgatttggtg tggaacttct
     7561 tgttatggac gtcgatgaac gccttcgcct caaccgcttc cttgaagcaa agcttcccct
     7621 agaactggaa ttccaggatt cgcacacaga actcacaatt agggcggcgg actaaatgca
     7681 acatcgctgc aattacaacg acttgcatgc aaatctccca ttaaaaaccg atacccacca
     7741 aggggttact ccattcaggt ctatcgaaga aatcatccag gttctaggct tacacgtagg
     7801 cactgaacaa tggaggaacc cacaatgagc tgttttcgtt gtaacggttg catggtcgaa
     7861 gattttctcc tcgatatgga agattcctca ggaccaatgt ggctccgcgc aaggcgctgc
     7921 atgaactgtg gaaatgtgtc ggaaagtgtg ttgcagcgca accggcagtc acaggaagcg
     7981 aagaatacct gccttgcggc gtcagggaag cgagaaatcg aaccttcagg cacaacaagc
     8041 gtagctgaat tggccgcgta aacaatcact gacgacctga caccaggaat aacctagcat
     8101 gtccattgat gtagagctgg ccctcgtatt gggattgttc atgatcggcg cggtggtgct
     8161 tgccatcgac aacctggtgt atgccttatc caagcgcttc agtcgatgga tgcgtaatcg
     8221 aaaatcagtc ccccgcaccc cttcgacctt cacctcgaca cagaagaaac aattttgatg
     8281 gaccccggaa gcaacccgca cggtccttcg cagatgcgag ggaccgacgc catcgcccag
     8341 acatttcaac ctccttaccc cgcaagcatt acgatagatt ccctctctgc ccaagccaag
     8401 aaggaggcac acatgacgtc ttgttcatct gacaaaattg cacgggaaag cgcacgaacg
     8461 cactctgcct caggcattcc tcagaccctc ctcttcttca tcttgaaaca actggaaaca
     8521 ggtcggcccg acgagcgggt tcaacaggat cacgcgcaat gatgatgagt cttaaggctg
     8581 tctttcgcga cgcagcgatc gtatacggtt gcacatttgc agccgcgttg ggcacggctc
     8641 tccttggagt gaatctgcag agcaatccgt caactgccta tatggcgaac ctgttagcgg
     8701 gtgcacttgg cttcacagtc gcaggcatac gcatagttcg cgatcgaacg gaacatctgg
     8761 catgggtggc ggtgatagtc tggattttca acttgccaaa cattgccctc ggcatacaaa
     8821 cgagtacatc gtggattcac agtggcctca ccctgatcct catggccgca ctgggtggca
     8881 cgctggccat gattctcacg cttacgtcct catctcctca taggacggat cccgggcgca
     8941 aggaagggcc ggcacatcag accggctctc gatagaccac cacagacgtg ggataattga
     9001 atagaactct ggcagatcga aggattggcc gcctaacttt tttcaggcgg cgacgcgatc
     9061 ggtgaaacag gcagttgccc ttcaggtgtc catccgccac ccagcgcttt gtacagttgc
     9121 acaatggaca ccagatgtaa ccgatgtgtt cccatcagtg ccaactcggc ctcaaaaaga
     9181 ttgcgttgag caattaagac atccagataa ttcgccaacc cgcctttata acgaaggtta
     9241 gccaggctca gtgctgatcg tagggcctca acctgctgca actgcgcggc gcgctgttca
     9301 cgcaccgtcc gcaccgccac caaagaatct tcgacttcct tgaaggccac gaggacggat
     9361 tgttcgtatt gggctaccgc ctgtcttgcc tgtgcctcgg cagctctctg ctgaaatcct
     9421 aggatctggg cattcaataa cggaccggcc aaccctggcc cggcaacccc gaaggccgtc
     9481 tcgtttgcga ctaaccggga cagatgcgga cttgccacac caagaattcc ggtaatactc
     9541 aattttggaa accgatcggc cttggccatg ccgattcgtg ccgttgccgc agccaggtct
     9601 tgttcagcct gcaaaatatc cggacgtcgt tggagcaatt cggaaggaag cccgggcggc
     9661 acgtccggcg gcatgacctg ctcagtaaga gagtagccgc gcgcgatacg tccgggatta
     9721 cgcccgagca atacactgag ctgattctcc ttctggacct tctgccgctc gaactccgcg
     9781 gccttcgctg ctgcgttggc ccgttcggct tcgaactgat cggcatcgag tttggagatc
     9841 atgccttgcc gcaagcgagc ctgggcaatt ctcaccgatt cctcccagga cttcagtgtg
     9901 cgccgggcaa tgtcgagctg catatcaaac tgcagcaggt cgaagtaggc ctccgccacg
     9961 ccactgacca gttgcaacac aatggcacgt cgattttcct cccgcgacaa gagatcgccg
    10021 cgcgccgcct cattggaccg tcggatcctt ccccaaatgt cgatttccca cgatagattt
    10081 ccttgtaagt agtagttgaa cgggttcgga aatccgggga agagaaaatt gctcttacga
    10141 ccgaagagtg gcgcgtttgc ggtcacattc atttgcggag catagtcggt cttcgcgata
    10201 aacaggcgcg cttgaaattc ttcgatggcc gccgcggcgc gtttgaggtc tttgttctct
    10261 tccaacgccg ttcggataag cttttgcagt tcctggtcct tcagcagttc ccaccagggt
    10321 gtattggcaa tcgatgcagc gtcaccgccc ggttcggcca tgcggaatgc atcgggcgtc
    10381 gttgcctcgg gacggttgaa atccggacca accgcacagg ctgctaaaag agtggagaaa
    10441 agcaccaatg ccaggctacg catgctgata atcacgctcc ttttcaggtt gatcaggtcc
    10501 atcggccccg ggaggaggaa gctgacggtt ccgtccgcga ctcaacgagc ggatcaaaac
    10561 aaaaaacagc ggcacgaaga aaattgccag aaatgtggct gccagcatcc cgccgaacac
    10621 tcccgtgcca atggaatttc tgctggcagc gccggcaccc gtggcaacca ccagaggaac
    10681 aacgccaaga ataaacgcca ttgacgtcat cacgatggga cggaagcgga gcttggccgc
    10741 atcgatcgtc ccctccaaca agggatgccc ttcctcatag cgcttgttcg caaattccac
    10801 gatcagaatg gcattcttcg ccgacagccc gatgagcgtc accagaccga tctggaagta
    10861 gatgtcattg gtcatcccct tgagccacac ggcactcaag gcgccgaaca gcccgatcgg
    10921 aaccgcgagg atcaccgcaa agggcaccac ccagctttca tattgcgcag ccaggaccag
    10981 gaacaccatc aacaatccga agccgaatgc gtagagcgac tgattgccga ccatccgttc
    11041 ctgataggaa atgccgctcc aatcgatccc gtatcccttc ggcaccagca cttctttccc
    11101 taacttatcc agcgcgtcta agacctgtcc ggaactgacc ccgggtgccg ccgaaccaag
    11161 caccagcgcc gtattgtatc cgttgaaatg tgtcacggga tcagggccgc tgctgaactc
    11221 cgtgctcacc accgtatcca agggaatcat cgtaggcccc tgcgcgccga cggcccgcac
    11281 ataaattttt gagatgtcgt caggagttga ccgatactgc gcctcggctt ctgtctgcac
    11341 ccggaagaca cgaccgaact tcacaaagtc gttgatgtac agattcccga aataggcttg
    11401 cagcgtatcg aagacctccg aaatagggac ccccaatgcc ttggcgcgct cccgattcac
    11461 cttggcgaag atcctcggag cactgacgcg gaaactggtt ccgatggccc cgatcgccgg
    11521 attctgccgc gccttggcca caaactcctg cgccaccgcg gagaacttat tgaagtcacc
    11581 gccgctcgga tcttgaagct gtatggaaaa tccgcccgtc gcaccaagcc cgcgaatcga
    11641 cggggcgttg aaggcaagga tcaacgcttc tggaatcttt gcaaattccc cgtaagccgc
    11701 cccgatcaag gctttcacat gttgctgcgg ggccgtacgc tcatcccaat gtttgagcgg
    11761 cacgaacatc gtcgcggcat tgggtccgcg tgtactaaac acgaagttct gcccggacag
    11821 cgcatcggtc gaatggatcg ccggattggc cagaaaataa ttctcgatct tgctcaatac
    11881 cgtatccgtg cgctgcttcg acgccccatc cggaagttgc acgattgtga tgaaatatcc
    11941 ctggtcctcc tccggcagga aactgttggg gatggtttta aacaacaaaa ccacgcccgt
    12001 aagcagtacc gcgaacaccg cgaccgacag caggcgtcgc gtcagtgcca ttcccaccgt
    12061 cgagatatat cgtccctgca tccatgaaaa tgtccgattg aacaggccga acaagccatg
    12121 gtgccgctct tcgcccggct tgagcacgag ggcgcagagc gcagggctga gcgtcaacgc
    12181 cacaaaaccg gagatgatta ccgcaatgga aatcgtgatg gcgaactgct tgtacaactc
    12241 cccggtaatg ccaccaagaa agccgacggg cacgaacacc gcacacaaca caagcacgat
    12301 ggcgatcacc ggccccgtca cctcttccat cgcccgcttc gccgcgttct tagccgacag
    12361 tttcccctgg gtcatgtgcc gttcgcaatt ttccacgacc acgatcgcat catccaccac
    12421 gatgccgatg gccaggacca tgccgaacag cgtgagggta ttgatcgaga accctagcgc
    12481 ctgcattccg gcaaatgtgc cgatgaggga caccggcacg gcgacaccgg gaatcaatgt
    12541 cgcgcgccag ctttgcaaaa agagatagac caccagaatg acgagcacca tcgcttctgc
    12601 cagcgtcttc acgacctctt taatggagac gtcgataaat cgcgtggtgt cataggggat
    12661 gtcgtacgac acgccggtgg gaaatgcctt ggagacctcg gtcaattcgg cgcgaacccg
    12721 tttcaccgtt tcgagcgcgt tcgcccccgg ggagaggaac gtcagcagaa acacattggg
    12781 cttgccgttc cagcgccctt ccagggtgta cgattgggcg cccaattcaa cacgagccac
    12841 atccttcaac cggaccatgg aaccattcgg catggcccgc acgatcatgt cctcgaagtc
    12901 cttcacttcc gtgagacgac cttgcgtaat gacaggaatc gtcagctcgg tacccttcaa
    12961 ggccggctca cggccaatgg ttccggcagg aaaatcacga ttttgttccc gcacgacatt
    13021 cgcaacatcc gtcggcgtga ggctgagttg ggccatgcgg atggggtcca gaatcagccg
    13081 catcgagtaa ttctgcgacc cgaacaccac cgcattgcct acgccacgaa ggcgcttcag
    13141 gttgtcgagg acccgcagaa tggcatagtt ggaaagatag accgtgtcat gcgtaggatc
    13201 agtcgaactc aaggccacga cggccaacag atccggcgac accttgttga tgctgatccc
    13261 ctgccggacc acttcgggcg gtaattgcgg ctccgcgagt ttcacccggt tctgggtttg
    13321 tacctgcgcg atatcgacat ccgtgccgat ctcgaaggtc aacttgatcg acatgt
//
LOCUS       DAOQEW010000105        13158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1359_length_13158_cov_9.212699, whole genome shotgun sequence.
ACCESSION   DAOQEW010000105 DAOQEW010000000
VERSION     DAOQEW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1359_length_13158_cov_9.212699"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLS35_11920"
     CDS             <1..230
                     /locus_tag="PLS35_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNI19420.1"
                     /translation="SKNDAADIVELVLNLLKGVLQKGDAVKIAGFGNFVVRSKGARKG
                     RNPRTGEEIGITPRRVVTFRPSQVFKKYVNS"
     gene            272..625
                     /locus_tag="PLS35_11925"
     CDS             272..625
                     /locus_tag="PLS35_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNI19421.1"
                     /translation="MGNDPRLGSKVFYKIGEVSKISKLPAYVLRFWESEFTFLKPKKS
                     RGNQRLYVQRDVDTVLEIKRMLYDEGHTLAGVKRYWARRGRATVKKSGSRKELAQRVR
                     GDLQAIIRMIDSHSA"
     gene            688..764
                     /locus_tag="PLS35_11930"
     tRNA            688..764
                     /locus_tag="PLS35_11930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:722..724,aa:Pro,seq:ggg)
     gene            927..1742
                     /gene="surE"
                     /locus_tag="PLS35_11935"
     CDS             927..1742
                     /gene="surE"
                     /locus_tag="PLS35_11935"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNI19422.1"
                     /translation="MARVRILVTNDDGIASPGIHAVAAALGTLGEVWIVAPDRERTAV
                     GHAVTLHKPLRITKMAPRVFMVNGTPVDCVNLALVKILPGPPALILSGINRGVNLGDD
                     VMYSGTVSGALEGTILGIPSVAVSQEGGETFRFEVGARYATRVAAQVLQHGLPAETIL
                     NVNIPDVPFRSIKGVKVTCLSRRRFNNPIVEKVDPRGRKYYWIAGTRQSWSRQQDADH
                     EALERRMVSVTPIHLDTTYHAMLEHFKAWEQPLSKLAASRRVVKARSSRRTRA"
     gene            1739..2374
                     /locus_tag="PLS35_11940"
     CDS             1739..2374
                     /locus_tag="PLS35_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNI19423.1"
                     /translation="MSALIEWLIEVLGRFVIATISTFGYTGIVLTMAIESACIPLPSE
                     IIMPFSGYLVFTGQFSMVGVTLAGAIGNVLGSLVAYYVGVWGGRPFVERYGRYFLVSS
                     HDMELADRWFAKHGEAAVLIGRLLPVVRTFISLPAGISRMDVKKFVVYSFVGALPFCY
                     ALAYVGLKMGEHWNELHQYFHHADLVIGVLLAMGLGYFLWSHWPRRRTSME"
     gene            2384..3844
                     /gene="cysS"
                     /locus_tag="PLS35_11945"
     CDS             2384..3844
                     /gene="cysS"
                     /locus_tag="PLS35_11945"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNI19424.1"
                     /translation="MLRIYNTLTGSKEPFESMVPGKVRMYVCGVTVYDYCHIGHARSA
                     LVFDVLRRYLEYSGFAVEFAKNFTDVDDKIIKRANEQGVSCDQITATYIKAYYDDMER
                     LGVRRATLEPRATEHIADIVALVDTLVAKGMAYRVNGDVYFQVDRYPGYGRLSKRNTD
                     DLQAGARVDVDERKRHPMDFALWKGSKPGEPSWNSPWGPGRPGWHIECSAMAMRHLGE
                     TFDIHGGGMDLIFPHHENEIAQSCGATGKEFARYWVHNGFVQINQEKMSKSLGNFFTI
                     REIFDKSEWPDAVTGEMLRYFLLSTHYRGPLDFSDQSLAEAKNALNGLYDLFERLKEP
                     APSHGAADQQMREASIRTRQAFADAMDDDVNTPNAVAALQKLRGDANKALDAGLSAEM
                     RHAVRNEFRSLGVVLGLFQQDSWQFKSPGSRETQGASGVSAKSLSDEEVADKIAARLA
                     AKQSKNYKLADELRAELASHGIIIEDRPDGTSRWKR"
     gene            3819..4601
                     /gene="rlmB"
                     /locus_tag="PLS35_11950"
     CDS             3819..4601
                     /gene="rlmB"
                     /locus_tag="PLS35_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNI19425.1"
                     /translation="MAPADGKDNTPDVIYGLHAVREALRAGARPIQRLLVLGTDRQFG
                     EIVRLAREQRIPVYVEPRAAFDRLVPSGHHQGVVGLVAAKSYSDPDAILASAQAPGQT
                     PLLVLLDGVEDPHNLGAIVRTAEASGVQGVFIPERRAVGLTSVVAKASAGAVDYMPVG
                     RVPNLSRWIERLQQAGIWVYALDAEAPKVYTKLDFRGPVALVFGGEGKGVRPGVRGAC
                     DDAAHIPMSGKVSSLNVSASAAIVLYEAVRQRRERGEDRVGH"
     gene            complement(4612..4896)
                     /locus_tag="PLS35_11955"
     CDS             complement(4612..4896)
                     /locus_tag="PLS35_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI19426.1"
                     /translation="MPTAARTTKGRTKAASSEPILPPRKRRPEALTTREQEILELIWT
                     GFKNKEIAQRLKISVKTVEAHRANMMKKIRVSNTAQLLKAAIQGKMLKLR"
     gene            5101..5889
                     /locus_tag="PLS35_11960"
     CDS             5101..5889
                     /locus_tag="PLS35_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNI19427.1"
                     /translation="MAGGVMIPYVMVFLFGAVIGSFLNVCIYRLPREESVAWPASHCP
                     ACRQPVAVYDNIPVVSYLLLRGRCRSCQVPISTQYPLVEAVNTIGYVLIFWMFDFTAE
                     AFVYSALLSALIVITGTDLSHTMIPDAVTLPGIAIGLICAAVILPIGLIDSLLGVLAG
                     GGILWFLAWVSPYVFGKEGMGGGDIKLMAMVGAFIGWQPVLLAIMIGSFLGSLVGVAL
                     IAAGLTKRDQYIPFGPFLAAGSLLALLFHQPLLNWYWSLISLPQ"
     assembly_gap    5923..6022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6023..6322
                     /locus_tag="PLS35_11965"
     CDS             <6023..6322
                     /locus_tag="PLS35_11965"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HNI19428.1"
                     /translation="ASELRMARQLAMARHERVRVVVNAAQSELRTECVDCETTVLRRY
                     EFARTGTVVDSMSTRPEILFQSSGRSATATTVVLIDSRRVTHQVTVSLTGRVSLS"
     gene            6319..6846
                     /locus_tag="PLS35_11970"
     CDS             6319..6846
                     /locus_tag="PLS35_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI19429.1"
                     /translation="MKQPEGKRATRQGLTTSTAGFTLVESMVALVVLSIGVIGTIAMC
                     EWAQRGLQRGAMTTTALALAESCLEAKRSLPWDQLLVDDLDQNGTLESLMRDDGLLDD
                     ETNGDGIFTGSVTRSNIRLVWTVELNRGGQPGTASLATIEARASFTTLGGQSREVRVR
                     TIRANPRAVGAMALS"
     gene            6843..7616
                     /locus_tag="PLS35_11975"
     CDS             6843..7616
                     /locus_tag="PLS35_11975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI19430.1"
                     /translation="MTPATLSDSQQTKPIRARWCLNTRGVSLIELLIALAISSVAIAA
                     AIQTSAAYGSRLSSQHATMTANQELRLGLDVLCSELRLAGGGLLSGDTVFSKMEVDEV
                     EFFANLNGSSTILTQIVEVGRQDLPVEDGADWPKGKQLLLCTAAHCVWNRLAADGRKQ
                     TLTLMTPTLEQYQPKTAIFLLNRVRYYLKRAEGTHRLMREVDGVASTLLSELSGMQLQ
                     YFNKSGGIANDARDVVRVRVTIQGARQGMNLSRDIAIRM"
     gene            7698..9131
                     /locus_tag="PLS35_11980"
     CDS             7698..9131
                     /locus_tag="PLS35_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19431.1"
                     /translation="MALLAVLMVVFLLTLLGMTSMQLAGQEVVGASALQEERLAHHAA
                     EAAVDVVMGWFHDPTLRPQEIGTTWFSKRLVNAQGDPSYVDAQGHGQFIGTSTAPDVV
                     FDAANIQHDRLLNDPQTGWFKSLKGLAKILKLRVYGATRPGLLCTVEVTAGAGPASRV
                     TKTLSLEFGAYAVPALHAPLQSGTLGSEAVQSRSGSVFTHWGEMVVRGRAYLPRPEDV
                     PVKSALAPVSGQVYDEMSHREDRWLTIRLGGDAFFAQLPAEAWSGVPLNVHAHQEPVP
                     GIKVDQWEYATLKRMAMQFGQVYGIDREGLLYAGGVIQAGMGRPASEVFASKGPGDHR
                     GLVFVDTLDGMPPRFDNLGTIVLDQDYAEGIFIVNAHVLWKAGRSGRALPALSPPPEG
                     QQSLGARVPVQLSGIHLQGVLYAAGDVRFAGHLKVYGGVAAQGAIMEAANGSGMLEVW
                     YNHDLREGFVQGLPVVFAAPGSWQTKL"
     gene            9199..10998
                     /locus_tag="PLS35_11985"
     CDS             9199..10998
                     /locus_tag="PLS35_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNI19432.1"
                     /translation="MIRASSPACLSNGLTLGKPRPTRSRPSGLYEPLVHQGFLRQEEL
                     ALAFSEALRRRLDVTTLLMDRYRIPKPAIGAVLAEFYECQFLAYDEQTIVEPELLKNL
                     SIDYLRMNHWVPLKRQAQGVEVLIDDPHNPDKLLDVRRAFPGQSVLYRVGLRADIERL
                     LSDACGREVGDPITDILGELVSEAQLEEQQNATIAAITENDSAIVRLANQIIAEAYRR
                     GASDIHIEPYADRKETAVRFRVDGTCFTYMKIPAAYRRAIVSRVKIMASLDIAERRKP
                     QDGKIRFKLTTGQEIELRVATLPTAGFNEDVVLRLLSANGPRRLEEFEFSVETRRVVE
                     QLAQKPHGIVLCAGPTGSGKTTTLHAILASINTDERKIWTAEDPIEITQDGLRQVQVH
                     PKIGLTFATTMRAFLRADPDVIMIGEMRDKETADIAIEASLTGHLVFSTIHTNSAVET
                     VVRLLDMGCDPFNFSDAMLGVLAMRLCKRICSHCREPYHPTRSEYDELVHAFGERDWA
                     SRQAAHDDTFTLFRGRGCEVCHHSGYRGRVPIHELMVVSDGMKALIQGRGRTGELLAL
                     AKSEGMQTLVQDGIGKVLQGLTTYQQVRAVAIK"
     gene            11334..11453
                     /locus_tag="PLS35_11990"
                     /pseudo
     CDS             11334..11453
                     /locus_tag="PLS35_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            11818..12342
                     /locus_tag="PLS35_11995"
     CDS             11818..12342
                     /locus_tag="PLS35_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI19433.1"
                     /translation="MSYTTEYIASSQLITPRQLGFTLLEAMIAAGVLSVGLLGLAGLS
                     GMSLGKNVDANDMSRVTNIAMDMAERIQSNRQRVLDYNATATNVACPQSVSTQRMAWG
                     DCTQWSALVTNSGLQSAVGTVTASRLDPDPTANPVTMNRFLVTITVNWRTQKTDVSTS
                     RNKSAVFTTVIAPE"
     gene            12360..>13158
                     /locus_tag="PLS35_12000"
     CDS             12360..>13158
                     /locus_tag="PLS35_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNI19434.1"
                     /translation="MMRRVTGNQQGFTLVELMAAVLITVVIVAATMTTVVTANRANVV
                     NSQVADTQQNVRLATDLLSRDIKLAGFNYNATDPATTAVGACNATIGAVTKPVGLLPQ
                     DQSPTTADTGPDGVSMVLPVMNTTGWTLTAAAGGTANAPTYDNSISLSGTAITEMVAQ
                     GLAVNSTISIGGAISKTVKSVNATSIGFGTGNYVSGQFPIGTPVYLLQCVRYQVIVNT
                     PATCGSNASCLVRDGVPLVDGVEDMQITYACDGCNQAAPNPLYPDGII"
BASE COUNT         2727 a         3339 c         4017 g         2975 t
ORIGIN      
        1 tgtctaaaaa cgatgcagcg gatatcgtcg aactcgtgct caatctgctg aagggagtgc
       61 tgcagaaggg tgatgcagtg aagattgcag gatttgggaa ttttgtcgtt cgaagtaaag
      121 gggcccgtaa aggacgtaat ccgcgaaccg gtgaagaaat cggcattacc ccccgtcgcg
      181 tggtgacgtt tcgacccagt caggtcttta aaaagtacgt gaattcctag tcgctacctt
      241 gccacgagca cacgcagcat gaggaccggt catggggaat gatcccagac tgggaagtaa
      301 ggttttttat aaaatcggcg aagtcagcaa gatttctaaa ttgccggctt atgtcttgcg
      361 gttttgggag tcggagttca ccttcctgaa gcccaagaaa agccgcggca atcagcgatt
      421 gtatgtgcag cgtgatgtgg atacggtgct ggagatcaag cgcatgctct atgatgaagg
      481 gcatacgcta gccggtgtga agcggtactg ggcccgccga ggacgcgcaa ccgtcaaaaa
      541 gagcgggtcg agaaaagaac tggcacaacg cgtgaggggc gatctccagg cgatcatccg
      601 aatgatcgat tcacattcag catgaactgt tccggtgatg cgggcggctg cgtccaaccg
      661 tgtgggtaac atcgatgtcg gccgtgtcgg ggcgtagcgc agcccggtag cgcactcgct
      721 tggggtgcga ggggtcgtgg gttcaaatcc cgccgccccg accatcggca cgatgtagtc
      781 catgaggtac cgttctaaga gacgttgatc attacgaccg accatcttca cgtatttgtg
      841 aagcttctcg ccttcttccc gccccgcttg aaccagcgat ttcagttctt cccgttcacg
      901 atgagttcca tgcataagga gtgtctgtgg cgcgcgtacg gattcttgta accaacgacg
      961 acggaattgc ctctcctggc attcacgccg ttgcggctgc gcttggcacc ctgggagaag
     1021 tgtggattgt cgcaccggac cgtgaacgaa cggcggtggg acatgcggtc accttgcaca
     1081 agccgctccg cattaccaaa atggcgccgc gcgtatttat ggtgaatggg actcccgtag
     1141 actgtgtcaa tctcgcgttg gtgaaaattt tgcctggccc tcccgcgttg atcctctcgg
     1201 gaatcaatcg cggcgtcaat ctcggcgatg atgtcatgta ttccggcacg gtgtcgggag
     1261 cgctggaagg gacaattctc ggcataccat cggtagcggt atcgcaggaa ggcggagaga
     1321 cgttccggtt cgaggttggg gcacggtatg cgactcgagt ggctgcacaa gtgttgcagc
     1381 atggattgcc ggcggaaacc attttgaacg tgaatatccc cgatgttccc tttcgttcca
     1441 tcaagggcgt gaaggtgacg tgtttgagtc gacgtcgatt caacaatccc atcgtggaga
     1501 aagtggatcc cagagggcgc aaatattatt ggatcgcagg gacgcgtcaa tcgtggagcc
     1561 gtcaacaaga cgccgaccat gaagccttag agcgccgtat ggtgtccgtc acgccgatcc
     1621 atctggatac gacctatcac gcgatgctgg aacactttaa ggcctgggag caaccgctct
     1681 ctaagctggc ggcaagccgt cgggtggtca aagcgcgttc ttcgaggagg actcgcgcgt
     1741 gagcgctctg atcgagtggt tgatcgaagt attgggccgg ttcgtcattg cgaccatttc
     1801 gaccttcggc tacaccggca tcgtcctgac gatggcaatc gaaagtgcct gtattcccct
     1861 gccaagtgag atcattatgc cgttctccgg ctacttggtg ttcaccggcc aattttcgat
     1921 ggtgggcgtc accctggccg gtgcgatcgg taacgttctg ggatccttag tagcgtacta
     1981 tgtcggcgtg tggggggggc gtccctttgt cgagcggtac ggccgctatt ttctcgtctc
     2041 ctcacatgac atggaattgg ccgaccggtg gtttgcgaaa catggagagg cggcggtgtt
     2101 gatcggccgg ctcttgccgg tggtgcgaac gttcatctcg ctcccggcgg gcatttcgcg
     2161 catggacgtc aaaaagtttg tcgtctattc atttgtggga gcgcttccgt tctgttatgc
     2221 gctcgcctat gtgggattga agatgggaga gcactggaat gaattacacc agtatttcca
     2281 tcatgcggat ctggtgatcg gtgtgttgct ggccatgggc ctcggttatt tcctctggtc
     2341 acattggccc aggcgccgca cgtctatgga gtaaggacgc aacatgttgc gcatctataa
     2401 tacattgacc ggaagtaagg aaccgttcga gtcgatggtc ccaggcaagg tccgcatgta
     2461 cgtgtgcgga gtgaccgtct acgactattg tcatatcgga catgcccgga gcgcgttggt
     2521 ctttgatgtg ctgcggcggt atttggaata ctccggcttt gccgtggaat ttgccaagaa
     2581 cttcaccgat gttgatgaca aaatcatcaa gcgagcgaat gagcaagggg tcagttgtga
     2641 ccagatcacg gcgacctaca tcaaggcgta ttacgacgat atggagaggc tcggagttcg
     2701 acgggccacc cttgagccca gagccaccga gcacattgcc gacattgtcg ccctcgttga
     2761 taccttggtg gccaaaggaa tggcttatcg cgtcaacgga gacgtgtatt ttcaagtcga
     2821 ccgatatccg ggatatggcc gcctttccaa gcgaaacact gacgatttgc aggccggcgc
     2881 ccgcgtggat gtggacgagc gcaaacgtca ccccatggat tttgccttgt ggaaaggcag
     2941 taaacccggt gaaccgtcat ggaacagccc gtggggtcct gggcgtccag gctggcatat
     3001 tgaatgctcg gcaatggcga tgcggcacct tggtgaaacg ttcgacattc atggcggcgg
     3061 gatggatctt atttttccac accacgaaaa cgagattgct caatcctgcg gcgcgaccgg
     3121 aaaagaattc gcgcgctact gggtgcataa cggctttgtg cagatcaacc aagagaagat
     3181 gtccaagtcg ttgggaaatt tcttcaccat tcgcgagatt tttgacaagt ctgaatggcc
     3241 tgacgcggtg accggcgaga tgttgcgata ctttctcctg tccacgcact atcgcggccc
     3301 cttggacttt tctgatcaaa gcctcgcgga agccaagaac gccctcaatg gactgtatga
     3361 tctcttcgag cgattgaaag aaccggctcc ttctcatgga gcagccgatc agcagatgcg
     3421 cgaggccagc attcgaacac ggcaggcgtt tgcggacgcc atggacgatg atgtgaatac
     3481 gccgaatgct gtggcggcat tacagaagtt gcgcggagac gcgaacaagg cccttgatgc
     3541 aggactgtct gctgagatgc ggcatgctgt gcggaatgag tttcgttcat tgggtgtggt
     3601 gctggggctg tttcagcagg acagttggca gttcaaaagt cccgggtcac gcgaaaccca
     3661 gggtgcctcc ggtgtgtcag cgaaatccct gtccgatgag gaagtcgctg acaaaattgc
     3721 tgcgcggttg gcagccaaac aatccaaaaa ctacaagttg gctgacgaat taagagccga
     3781 gttggcatcc cacggcatta tcatcgagga ccggcctgat ggcaccagcc gatggaaaag
     3841 ataacacgcc ggacgtaatt tacggcctgc atgccgtgcg cgaggcactc cgtgcagggg
     3901 ctcgccctat tcagcgactg ctggtgttgg ggacggatcg gcagttcggg gaaatcgtgc
     3961 ggctggccag agagcagcgc attcctgtgt atgtcgagcc acgcgctgct ttcgatcgac
     4021 ttgttccctc cggccatcac cagggcgtgg ttgggttggt cgccgccaag tcttatagcg
     4081 atccggatgc tattcttgcc tccgcgcaag cccccggtca gactccactg ttggtactgc
     4141 tcgacggtgt cgaagatcct cacaatctcg gggcgattgt acgaacggca gaagcgtccg
     4201 gggtgcaggg ggtttttatt ccggaacgcc gtgcagtggg gctgacgagt gtggtggcca
     4261 aggcgtcagc cggagcggtg gattatatgc cggtcggcag agttcccaac ctttcgcggt
     4321 ggattgagcg cctgcagcag gccgggatct gggtgtatgc gctggatgcc gaagcgccga
     4381 aggtgtatac gaaactggac ttccgggggc ctgtcgcgct ggtatttggt ggagaaggaa
     4441 agggagtacg gccaggggtt cggggcgcct gcgatgatgc tgcgcacatt cctatgtcgg
     4501 ggaaggtcag ctcgctcaat gtctcggcgt cagcggccat cgttctgtat gaggctgtgc
     4561 gtcagcgtcg ggaacgagga gaggatcgag tcggacactg agaccgctgg actatctcag
     4621 cttgagcatt tttccttgaa tggcggcctt gagcagctgg gccgtgttcg acacccggat
     4681 cttcttcatc atgttggctc ggtgtgcttc gacggtcttc acgctgattt tgagccgctg
     4741 ggcgatttcc ttgttcttga agccggtcca gatcaattca agaatttcct gttcgcgtgt
     4801 ggtgagggct tccggacgtc gcttgcgagg gggaagaatc ggctcggatg atgcggcttt
     4861 ggttcgacct ttggtagtcc tggcagctgt cggcatgtac gtctcgctcc acttggaata
     4921 tggcaattct cagattgaat cttcgactac tcggcccaaa acgttgtcgg attatacgca
     4981 gtgctcctgg cgtttgtaaa tgaaaagagg cgggtgcaga ggggggaccg catgctctta
     5041 ttgacctagg cgcgtatacg tgtacttaga tgcacgtgcg tggatggaaa ggagcatgcc
     5101 gtggcgggag gcgtgatgat cccatatgtg atggtgttct tgtttggtgc ggtcatcggt
     5161 agtttcctga acgtctgtat ctaccgatta ccgcgcgaag agtctgtggc ctggcccgcc
     5221 tcgcattgcc ccgcctgtcg gcaacctgtc gcggtatacg acaacattcc tgttgtcagc
     5281 tatctacttc tgcgggggcg gtgccgttcc tgccaggtgc ccatttccac ccaatatccg
     5341 ttggtggaag ctgtcaacac aatcggatac gtgctgatct tttggatgtt tgacttcacg
     5401 gccgaggcgt tcgtgtattc cgccttacta tcggcattga ttgtgattac ggggacggat
     5461 ctttctcata cgatgattcc tgatgctgtg accttgccgg gaattgctat cggcctcatc
     5521 tgtgccgcgg tgattctgcc gattggcctg attgactcac ttcttggcgt cctcgcgggt
     5581 ggaggtattc tgtggttcct ggcttgggtc agcccctatg tcttcggaaa agaaggaatg
     5641 ggcggcggag acattaaact catggcgatg gtcggtgcgt tcattgggtg gcagccggta
     5701 ttgttggcca ttatgatcgg atcgtttcta ggctccctcg tcggtgtcgc gctgatcgcc
     5761 gccgggctca ccaaacggga tcaatatatt ccatttggac cgttcctcgc ggcggggtcc
     5821 ctgctcgctc ttctgtttca tcagcctctc ctcaattggt attggtctct cattagcctg
     5881 ccccagtaaa tctctgcaaa tcggctgtca gagccagttt gtnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nngcatctga attacgcatg gcccggcagc tggccatggc
     6061 gcggcacgaa cgtgtgcgag tcgtggtgaa tgcggcgcaa tccgagttgc ggactgaatg
     6121 tgtggactgt gagacgactg tgctccgtcg ctatgagttc gcacgcacag gcacagtggt
     6181 cgactcgatg agtaccaggc ccgagattct ttttcaatcc agcggccgtt ccgccacggc
     6241 cacgaccgtg gtcttgattg attccagacg cgtgacgcat caggtcacgg tgagcctcac
     6301 aggacgggtg tcactgtcat gaaacagcca gaggggaagc gggcaacaag gcaaggtctg
     6361 accacgagca cagcggggtt tacgctggtg gaaagcatgg tggccttggt ggtcctgtcg
     6421 atcggagtca ttgggacgat cgcaatgtgt gagtgggcgc agcgtggcct tcaacgcggt
     6481 gcgatgacga cgacagcgtt ggctctcgca gaatcgtgcc tggaggcgaa gcgtagtctg
     6541 ccttgggatc aattgttggt cgatgacctc gatcagaacg ggacgctgga atctctcatg
     6601 cgcgacgacg gcctgctgga tgatgagacc aatggcgacg gtatcttcac cgggagtgtg
     6661 acgcggtcca acatcagact ggtctggacg gtggaactca atcgtggagg gcagcctgga
     6721 actgccagcc tggcgacgat tgaggcgcga gcgagtttca cgacgctcgg cggccagtcg
     6781 cgtgaagtgc gcgtgcgtac gatccgggca aatccgcgcg cggtcggtgc gatggcgctc
     6841 tcatgacacc cgccacactc tctgattcac aacagacgaa gccgatcagg gcgagatggt
     6901 gtctcaacac caggggggtc agccttatcg agcttctcat cgcgctggcg atcagcagcg
     6961 tggcgatagc ggcggcaatt caaacctctg ccgcctatgg ctctcgcttg agttcccagc
     7021 acgctaccat gacggccaat caagaactgc gactagggct ggatgtgttg tgtagcgaac
     7081 tgcgcttggc cggtggcgga ttattgagcg gagacactgt cttttcaaag atggaggtgg
     7141 acgaagtcga attttttgcc aatttgaacg gatcgtcaac catcctgacg cagatagtag
     7201 aagtcggccg gcaggacctg ccggtagaag acggcgccga ttggccgaaa ggcaagcagc
     7261 tgcttctgtg tactgcggcg cattgtgtct ggaatcgtct ggctgccgat ggccgaaagc
     7321 aaacgctgac gctcatgacg ccgactctcg aacagtatca gccgaaaacc gccatctttt
     7381 tgctgaaccg tgtgcggtac tacctaaagc gtgctgaagg aacacatcgg ctgatgcgag
     7441 aagtggatgg cgtggcaagt acgttgttga gcgagttgag cggaatgcag ctgcagtatt
     7501 tcaacaaaag cggaggtata gccaatgacg cgcgggatgt ggtgcgcgtt cgagtaacga
     7561 tacagggggc tcggcaagga atgaacctgt cgcgagatat cgcgatacga atgtgactaa
     7621 tgtaactaaa ggggaccggg gatatgaata ggagcggaga acgatcagca aagagaaggc
     7681 agggggatga acggggaatg gcattgttag cagtgctgat ggtggtgttt ctcctcaccc
     7741 tcttggggat gacatccatg cagttggcgg ggcaggaagt cgttggtgcc agtgcattgc
     7801 aggaagagcg gctcgcgcat cacgctgctg aggcggctgt ggatgtggtg atggggtggt
     7861 ttcatgatcc gacacttcgt ccacaggaaa tcgggacgac ctggtttagc aaacgactgg
     7921 tcaatgcaca gggagatcct tcatatgttg acgcgcaggg gcatggacag ttcatcggaa
     7981 cgagcacggc ccctgacgta gtgttcgatg cagccaacat ccagcacgac cgactcctga
     8041 atgatccgca gacaggctgg ttcaaatcgc tcaagggtct cgcgaaaatt ctcaaattac
     8101 gagtctatgg agcgacgaga ccgggtttgc tctgcacggt cgaagtgacg gctggggccg
     8161 gccctgcctc ccgtgtgaca aaaacgttga gcttggaatt cggggcctac gccgttcctg
     8221 ctcttcatgc gcctctgcaa agtggcaccc ttggcagcga ggcggtgcaa tcccgttccg
     8281 gttcggtttt tacgcattgg ggtgaaatgg tggtgcgcgg acgagcctat ctcccccgcc
     8341 cggaagacgt tccagtcaag agtgcattgg ctcctgtgag tggacaagtc tatgacgaaa
     8401 tgagtcatcg ggaggatcgc tggttgacga tccggctggg gggcgatgct ttttttgccc
     8461 agcttccggc cgaagcttgg tccggcgtgc cattgaatgt gcatgcgcat caagagccgg
     8521 tccccggcat taaggtggat cagtgggagt atgcgacctt gaagcgaatg gcgatgcagt
     8581 ttggccaggt gtacgggatc gatcgagagg gattattgta tgcgggcggt gtcattcagg
     8641 ccggcatggg gcgtcctgca tccgaggtgt ttgcttcaaa gggaccgggc gatcatcgtg
     8701 gactcgtgtt tgtagacacc cttgatggga tgccgcctcg cttcgataac ttgggcacga
     8761 tcgtgctcga tcaggactat gccgagggta tctttattgt gaatgcgcat gtgctgtgga
     8821 aggctggccg ttcggggagg gccttgccgg cactaagccc gccgcccgag gggcagcagt
     8881 cgcttggggc acgggttcca gtgcagctct cgggcattca tcttcaaggc gtgttgtatg
     8941 ccgcaggtga tgtgcgattt gccgggcatc tcaaagtcta cggtggggtg gcggcgcaag
     9001 gagcgattat ggaagcggcc aatgggtctg gaatgctgga ggtttggtac aaccacgatc
     9061 ttcgtgaagg gtttgtccag gggttgccgg tggtatttgc cgcacccggt agttggcaga
     9121 ctaaactgtg agaggatgag cgccctctcg caatcagatc atcaccccat gtttaaatca
     9181 gttgaaaagg atagggccat gattcgtgca agttcccctg cgtgtttgtc aaacggactc
     9241 acattaggaa aaccacggcc aactcgtagc cgtccatccg gactatatga gccactcgtt
     9301 caccagggat ttctccgaca ggaggaactg gcgctggcct tctcggaagc gttgagaaga
     9361 aggctggatg tgaccacgct actcatggac cggtacagaa ttcccaaacc ggcgattggc
     9421 gcagtccttg cggaattcta tgaatgccag tttctcgcct atgatgagca gactatcgtg
     9481 gagccggagt tattgaaaaa cctgagcatt gactatctga gaatgaatca ttgggttccc
     9541 ctcaaacgtc aagcacaggg ggtggaggtt ttgatcgatg atccacacaa ccctgacaag
     9601 cttctcgatg tgcggcgcgc ctttccaggg cagtctgtcc tataccgcgt tggtctgcgg
     9661 gccgatatcg agcgcctgtt gagtgatgcg tgcgggcgtg aggtcgggga tcccatcacg
     9721 gatattcttg gcgagttggt gagcgaagcg cagttagaag aacagcaaaa tgccacgata
     9781 gcagcgatca ccgaaaatga ttcggccatc gttcgtctcg ccaatcagat catcgccgag
     9841 gcctatcggc gaggtgcatc ggatattcat atcgagccct atgccgaccg caaagaaacg
     9901 gccgtgcgtt tccgggtcga tgggacctgc tttacctaca tgaagattcc tgccgcgtat
     9961 cgtcgggcga ttgtatctcg cgtgaagatc atggccagcc tggatattgc cgaacggcgg
    10021 aagcctcagg acggaaaaat ccgcttcaaa ttgacgaccg gccaggagat tgaattgcgt
    10081 gtcgcgaccc ttcccactgc aggatttaat gaagatgtcg tgcttcgcct gctgagcgct
    10141 aatggcccca ggcgactcga agagtttgag ttcagcgttg agacgcgccg cgtagtcgag
    10201 cagttggcgc aaaaaccaca tggcatcgtg ttgtgcgccg gcccgaccgg atccggaaaa
    10261 acgacgacat tgcacgccat tctggcatct atcaatacgg acgaacgaaa gatctggacg
    10321 gccgaagacc cgatagagat tacgcaagac ggcctgcgac aggtgcaggt gcatccgaag
    10381 atcggcctga cgtttgccac caccatgagg gcctttttgc gagccgatcc cgatgtcatt
    10441 atgatcggtg aaatgcgcga caaggaaacg gcggatattg ccattgaagc ctcgttaacg
    10501 ggtcatctgg tgttcagcac catccatacg aatagcgccg tagaaacagt cgtgcggttg
    10561 ctcgatatgg gctgtgatcc attcaacttt tctgatgcca tgctaggtgt ccttgcgatg
    10621 cggttgtgta agcgaatttg ttcgcactgt cgggagccct accatccgac tcgcagcgaa
    10681 tatgatgaat tggtgcacgc gttcggagag agggactggg catcccggca agccgcgcat
    10741 gatgacacgt tcacgttgtt tcgcggacga gggtgcgagg tgtgccatca cagcgggtac
    10801 cgcgggcgag tgccaattca tgagttaatg gtagtttccg acggcatgaa agcgctcatt
    10861 caaggacggg gtcgcaccgg agaattgctg gcactcgcaa aaagtgaagg tatgcagaca
    10921 ctcgttcagg atggcatcgg gaaggtcctc caaggcctca cgacatatca gcaggtgcgc
    10981 gcagtggcta ttaagtagcg ttgttgtccg tagttgttga tgaatctgcc tcaaagcagg
    11041 tccactcgca gaatatggtg tcagttcgtt cggcctgcga tcaggaatgt aattcccttt
    11101 tcttccaatt ttggagtcct tgtcccgggc tcatcacgcc tcccttctcg tgctgttgac
    11161 cctccccgat gggcaaagat cgccatcgac cagacgaaaa ttgtcatctc ggtgatcaga
    11221 aaacgctccg aagtgtacgg gcgcggatat gaatgcgctc gagatatcaa ttggttaggt
    11281 acatagtctt ctgaaaatgc atcggcatgg cacttgctca tctgcctgtt gctatgtgtg
    11341 tctttcccta tcaccaacgg tcctctgttg tccgttgtca gagaggtttc acgctcatcg
    11401 aagtgatgat cgccgtagcg attgttggca tcttggccat ggtcgccgtc cctaattatc
    11461 tgcaatggaa tgcccgctat cagctgaagc agggcacgac cgaattggca ggcagtctga
    11521 cgctggcccg tatggccgcg atgaatcgga atctggccgt cacggtgacc ctggcgttgg
    11581 cttccgggag agtgaccgtc gatttcggcg gggcactcgc tcccatcgtc ctgccccaag
    11641 cgatagttgg atttaccggg ggccccacag tgcagttcac ccgacaagga ttaagcggat
    11701 caaccgcaaa cgtgccgctg accctggttt ctcaacaagg gaccgtctac tcgctggtcg
    11761 tcaccccgtc aggcaaagtg aactggtgcg cccacgccac gtgcccatag aggctgcatg
    11821 agctacacga ctgaatacat agcgtcctca caattgatca cgccgcgtca gctaggattt
    11881 accttgttgg aggcgatgat cgcagcgggc gtgctttctg tcggtctact aggacttgcg
    11941 ggattgtcgg gaatgtctct ggggaaaaat gttgatgcca acgacatgtc gcgtgtcacg
    12001 aatattgcca tggatatggc tgagcgtatt cagagtaacc gtcagcgggt gttggattat
    12061 aacgccactg cgacgaacgt ggcctgccca cagagtgtga gcacgcaacg catggcgtgg
    12121 ggcgactgta cccaatggtc agcgttggtc acgaactccg gtctccagtc ggctgttggt
    12181 accgtgacag ccagccgtct cgatcccgat cccacggcaa atcctgtgac gatgaatcgc
    12241 tttttggtca ccatcacggt gaactggcgt acccaaaaga ccgatgtgag cacctcgcgc
    12301 aacaagagcg cggtgtttac gacggtgatt gcaccggaat agacgcgatg gagagcatca
    12361 tgatgcgtcg agtgacaggc aaccaacagg gcttcacgct cgtggaattg atggccgcgg
    12421 tgttgatcac cgtggtgatc gtcgcggcga ccatgacgac ggtggtgacg gcgaaccgag
    12481 ccaatgttgt gaatagtcag gtggctgaca cccaacagaa tgtccggttg gccactgatt
    12541 tgctgagccg cgatatcaaa ctggcagggt tcaattacaa cgccactgac ccggccacca
    12601 ccgcggtagg ggcatgcaat gcgaccatcg gtgccgtgac caagccggtt ggccttcttc
    12661 cgcaggatca atcacctact acggcggata ctgggcccga tggcgtttcg atggtgttgc
    12721 cagtcatgaa caccacgggg tggaccttga cggcggcggc aggtggcaca gccaatgcgc
    12781 cgacctacga caattccatc agcctttcag gcacggccat tactgaaatg gtcgcccagg
    12841 gcctcgcggt caattcaacc atttccatcg gtggagcgat ctcgaagact gtgaagtccg
    12901 ttaacgcgac atcaattgga ttcggcacgg gcaattatgt gagtgggcaa tttccgatcg
    12961 gcacgccggt gtatttgctg cagtgtgtcc ggtaccaggt catcgtgaat acgccggcca
    13021 catgcgggag caacgcatcg tgcttggtca gggatggtgt gccactcgtt gatggcgtcg
    13081 aggacatgca gatcacctat gcctgtgacg gatgcaacca ggctgcgcca aatcccctgt
    13141 atccggacgg tatcatcg
//
LOCUS       DAOQEW010000106        13061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1370_length_13061_cov_8.074658, whole genome shotgun sequence.
ACCESSION   DAOQEW010000106 DAOQEW010000000
VERSION     DAOQEW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_13061_cov_8.074658"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..554)
                     /locus_tag="PLS35_12005"
     CDS             complement(60..554)
                     /locus_tag="PLS35_12005"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI19435.1"
                     /translation="MTKPAKNTICLWYDRDAEEAARFYAKTFPDSFVGAVHRAPGDYP
                     SGKKGDVLTVEFTVMGIPCLGLNGGPAFKHNEAFSFQVATTDQAETDRYWQAIVNNGG
                     EESECGWCKDKWGLSWQITPVVLTDAVTNSDPAVAKRAFDAMMQMGKIDVATIEAAVR
                     GSKT"
     gene            complement(647..1054)
                     /locus_tag="PLS35_12010"
     CDS             complement(647..1054)
                     /locus_tag="PLS35_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNI19436.1"
                     /translation="MRFLVLMIPGVYQPNTNRQPKANFTPTAEMMAKMGRFNEELKQA
                     GALLSLDGLQPLATGARLAFSNGKASVTDGPAVDAKEVVGGFWMLQATSKQQVIDWMT
                     RCPALDGDVIEIRQVFELSDFQEDVQATAKKHG"
     gene            1358..4297
                     /locus_tag="PLS35_12015"
     CDS             1358..4297
                     /locus_tag="PLS35_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNI19437.1"
                     /translation="MTFLSRYRLLLYVLSGLVALYALLGFVVAPYAVKTYGIPALSER
                     LVHPVVLGDMRLNPFTFSIELTGFEIQEPDHTPMIGFQELFVDFEATSLVRSSYLFDE
                     IRLTLPFGLVHLQADGKLNLLGLVPPGSQEAERPAPPVEQPRKEPMPSIEIRQLSIQR
                     GVIEYRDDSKKRPVTIDVVPIEISVRNFTSRGRGENAYAFTAEFGEGEVLAWEGTLHL
                     DPLESDGHVSLSHVGLKTFWPSLRDRFRFDILSGTLLVDVRYHFDTHLAPINLQVTEG
                     KVQLADFRLSEAGSRDPVVTVPSFGIEGINMDLSKRELGIGTVALTGAEVRAWLGQDK
                     VVNFQPLFAPVAATADASPPSEPPAPDAGQAWKVAVQSVEVANTQVAFEDRSLATPVE
                     LLLDDVHLTAGDVHVPFKGAVPVTAGFRLNQQGALEAKGTLRLDSLQAALALKLDHIG
                     LRPFQPYLDRFVQLEVRDGELMLDGDLVYRSRHESEPMLQYRGQAGLNNLHVADRAST
                     KEFLGWTALSLSKMALDVAPTTVKIGEIALRDPAFRLITDKDGSTNLSRVLRPSDTPP
                     APASKPVEPEAPKKPATPTPVEIAVVRLTNLSATFSDESIVPAVSTGITDLTGTIKGL
                     SSRQIAKAEVALTGKVDEVAPLKIQGQINPLSEDTFTHLTFVFQGVDLTSVSPYAGKF
                     AGYPITKGKLSLDLMYQISKKQLVGENKVLVDQLTFGDKTDSPDATTLPVRLAVGLLK
                     DRRGRIDIDMPVRGDLNEPDFRYGRVVLNALVNLLTKVVTSPFSALGGLVGGGGEDLQ
                     FVEFQAGSDSLAEPEQRKLESIAKALEERPALRVDVTGVADPVRDRQALALQKIADEV
                     LRRFTKGGTKNLQAVPSPSREFELLSDLYAEKLGKQPMKREELPGGKSVERVLTADEL
                     RQQLFPAMTVEDAELRGLAQERAKAIREHLIAQGHLPDERVFLVEAELAASDKPQIRV
                     HLSLTGS"
     gene            complement(4312..4671)
                     /locus_tag="PLS35_12020"
     CDS             complement(4312..4671)
                     /locus_tag="PLS35_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNI19438.1"
                     /translation="MRQPATGLRIANDPMYRLLREGCIKEFNVKRAAGEPVDLTRCDL
                     RGLDLRGLEVDGLDFSECYFRQADLRGIDFRKAKLEGASINAAKISGAYFPIELTASE
                     IELSLLHGTRMRYQPKP"
     gene            4877..5512
                     /locus_tag="PLS35_12025"
     CDS             4877..5512
                     /locus_tag="PLS35_12025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNI19439.1"
                     /translation="MGLRLGDDAPNFTAETTDGTINFHEWLGNGWGILFSHPKDYTPV
                     CTTELGYMARIKGEFDKRGVKILAISVDPLDSHRGWMKDINETQNCTVTYPIIADPEK
                     KVANLYDMIHPNSLDSMTVRSVFVIGPDKKIKLMLTYPASCGRNFDELLRVVDSLQLT
                     AKYKVATPVNWKDGQDCIITPAVNDAEAKTLFPKGFKSVKPYLRLTPQPNK"
     gene            complement(5690..6520)
                     /locus_tag="PLS35_12030"
     CDS             complement(5690..6520)
                     /locus_tag="PLS35_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19440.1"
                     /translation="MMTPRLLTLLMATGIVCAAPLSASAIAVYSSSGSNAASIQGSVD
                     GFRADLGTNNLANAPATGGRREINWDGVPAGFSDPNHLPGNFFNSNSPRGAVFETPGS
                     GFKVSANTGVGTPERFGSINPSFPSSFSVFSSQKLFTAIDSNIVDVKFFVPGTTQAAN
                     VRGFGSVFTDVDLLGPTTVEFFDMNNNLLSKQTVLNTPGATSLSFLGVSLEQAIINRV
                     RITAGNAALSNLDGFDFVAMDDFIYGEPQAVPAPAAVLLLASGLAALVWARRNSLLPE
                     "
     gene            complement(6780..8972)
                     /locus_tag="PLS35_12035"
     CDS             complement(6780..8972)
                     /locus_tag="PLS35_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI19441.1"
                     /translation="MALPRVLLIIPPLTQLNTPYPATAYLTGFLRSRGYEAEQADLGL
                     EMILRLFSRVGLQEAFHRIKASRRPWRGEVQQFLALEDSYLATIDPVIAFLQGKDPML
                     APHLARPGFLPEGPRFGADGRPGSSPFPTSPTDSARHRATLYLEDLTDLLYETIAPHL
                     FLNRYAEQIMQATSSFDGIEKALAEPLSFTDELLLEALAPLLAGSSPDVVGLTVPFPG
                     NLFGALRIAQAIKAQRPATAVVLGGGYVNTELRRLKDPRLFRYVDYVTLDDGERPFLC
                     LLDFLAGGRSQATLCRTFVLADGQVVFQNGNTEPDLSMHELGTPTYNGLPLGRYLSIL
                     DSLNPMHRLWSEGHWNKLTVAHGCYWKQCTFCDVGLNYIARYEMTPTDVLIRQIEALI
                     RETGQRGFHFVDEAAPPAALKALALRLLETNQTISWWGNIRFEEAFSPDLARLLAASG
                     CIALTAGLEAASDRLLRQMKKGITVDQTARVAAAFRHAGILVHAYLMYGLPGESIADT
                     VDSLERVRQLFANNLLQSAFWHRFTATAHSPVGLNPAAEGITILGPTFEGFAENDLRH
                     ADPHASCPPWLGEGLRTAILNYIEQAGLTVPVQQWFPQAVQAPRVAKTWLTRALADAV
                     PEPLTAERRCVWIGGRPVVAAKGTTRVKIILPTRMEDFSISVSSDQVDWLMDLLQNAS
                     PSPHRTDRDYPRFAEARERFPHTGARGFDRFARTAAWHNARQAGLLLV"
     gene            complement(9037..10377)
                     /locus_tag="PLS35_12040"
     CDS             complement(9037..10377)
                     /locus_tag="PLS35_12040"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNI19442.1"
                     /translation="MALAKTSDDSRKLDKSDTLVLSPERLRVGMYVDLNCGWFRHPFP
                     RRSFKLSSASQIATIKGLGLTTVLVYPRESDPDPDAEGTLLETDPASTATVEPDTGSA
                     PAAAAADTNPPPSDEELPTHGDYHQAVELASETYRQMLGTSKDMMDALTSGSEEGMRS
                     AETMIGGISTLLTHTQAAGTVASAFDPEEIGDAGVLHSLNVATLSMMVAQQFDIDQDA
                     LQMIGLAGLLLDIGERRIPRHILDTRARRSETEHVKYQLHPYLGVEMLRGFPGIPEEV
                     LDVIRSHHERLDGSGYPEKLKGDQISFPTRIVSAVDHYDTLVNSLYAEEALSPAEALA
                     TMYKHEKQLFSTEVVVALIHTLGVYPPGTVVSLSDGSFGLVLNINFKNRMKPLVLMYH
                     PTPGQEPHTIDLLRDQTLSILRAVPREELPRPIVEFFRLKRWTGYFIQATMRTA"
     gene            complement(10621..11769)
                     /locus_tag="PLS35_12045"
     CDS             complement(10621..11769)
                     /locus_tag="PLS35_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HNI19443.1"
                     /translation="MSDQQLHFSGQRFSVEYHIQGPKEEACRRAAVLCIDQTVEAADH
                     VIPSGVIRDQLLGQMLSIDRVGDGVHRATLTFPIELLDGTMGTLQHMSFGMAGLQSRV
                     RLANLSLPDEAIAQMAGPRFGSLKLRALLEVPVRPLVCAVLKPLGLSARALASLAYEF
                     ALGEVDLIKDDQSLGDHPFCRFEERVTLCAEAIAKASRQTGRRCLYAPHVSGPWPTLL
                     DRASLAQNAGAGALLLCPGLTGFDALGTLARLPFPSLPLIAHPDFQGSHFVNSATGIA
                     PAVLFGLFPRLAGADVSIYPTFGLEYPISQEDCRGIAACCRNSLGACAPIFPTAAGRM
                     EASRIHEMVAFYGTDVVFVLGSDLRRDPTRIRSACQEFIQLLEAEVAP"
     gene            11869..12696
                     /locus_tag="PLS35_12050"
     CDS             11869..12696
                     /locus_tag="PLS35_12050"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNI19444.1"
                     /translation="MVIKPRHLRWLGLSALTLCVVMHVSAVSGSHAAEIGPRVLDLTY
                     PFDETTQVWPSNPPFHRDSTQRGGTTTEAWYATGQVALSEHAGTHMDAPIHFAQGQAG
                     IDGIPVEQLMGPAVVIDVRAAAADDRDYRLSLADIHRWEAAHGTIPTGAIVMMLTGWG
                     AYWNDRQRYFGSATPEIPTTLHFPGFSKEVAEFLVRERHISGIGIDTASIDYGPSQDF
                     VVHRIVNAAGLYGLENVAGLAEVPASGARLVALPMKIAGGTGAPVRIIAILPSRDGK"
     gene            12693..>13061
                     /locus_tag="PLS35_12055"
     CDS             12693..>13061
                     /locus_tag="PLS35_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNI19445.1"
                     /translation="MTSRKTSCRVYSDFNCPFCYAMHERLYALGFLEQISWHGVQHAP
                     HLPQPMATWAGHLHAELKQEVKMVRRLAPELPISVPAGKPNTRLAITVAARALREDTR
                     RAGEFIRSLYRLFWVDGLDLS"
BASE COUNT         2733 a         3590 c         3994 g         2744 t
ORIGIN      
        1 cttctaagct gagggtcgct ggttcgattc cagccgggcg caccacaaga gactctaccc
       61 taggttttgg aacctcgaac agctgcctcg attgtcgcga cgtcgatctt tcccatttgc
      121 atcatggcat cgaatgcgcg cttggcgacg gcgggatcgg aattcgtaac cgcgtccgtc
      181 aagactaccg gcgtgatctg ccacgacagg ccccatttgt ctttgcacca gccgcattcg
      241 ctttcttcgc ccccgttgtt gacgatcgcc tgccagtatc gatccgtttc ggcctgatcg
      301 gtggtcgcaa cctgaaacga gaatgcttcg ttgtgcttga aggcgggccc gccattgagc
      361 ccaaggcagg gaatgcccat cacggtaaat tcaaccgtca gcacatcgcc tttcttgccg
      421 gaaggatagt ctccgggtgc cctatggacg gcgcccacga aggagtcggg aaaggtcttg
      481 gcgtaaaaac gcgccgcctc ctcagcatcg cggtcgtacc acaggcaaat ggtattcttt
      541 gctggcttgg tcatatcgtt ctctccttca tgtttcggtt ttacgtgaat cacaggctcc
      601 cagcaggata ggcactcttg cgtccagttc tgcaacccgc tgccactcaa ccatgttttt
      661 tcgccgttgc ctggacatcc tcctgaaaat cggacaattc gaagacctgc cgaatttcga
      721 tcacgtcgcc atccagcgcc gggcagcgtg tcatccaatc gatgacctgt tgctttgatg
      781 tagcctgcag catccaaaag cctccgacca cttccttggc gtccacggca ggaccatcag
      841 tcacgctggc cttaccgttg gagaatgcca ggcgggcgcc ggtcgcgagc ggctgcagtc
      901 catccaatga aagcagcgca ccggcctgtt tgagttcctc gttgaacctg cccatcttcg
      961 ccatcatctc tgcagtaggc gtaaaattgg ccttcggctg cctgttagta ttgggctgat
     1021 agacgcctgg gatcatgagt actaagaagc gcatatgatt tctcctttct tgtatattcc
     1081 actcttcacc tagtagtcgt cgtccggttc gcaaatcgac atggtgcata caagccctca
     1141 acattcgctc cattaccgat gtttctcccc ggttgacagt gtctggatgg cacgtagttg
     1201 taatccatga gacattttct ccttgttcta ggcggtctta gtagttatcc ttcccattcg
     1261 atccctttac tcacgctgca gttcaaagga ctttgcaagc agagggttgc tggctcgatt
     1321 ccagccggtg cccccacctt cctgatgtgt gcctgacatg acgttcctct cccgctatcg
     1381 acttctcctg tatgtgctca gcggcttggt ggcgttgtat gcgctactag gttttgtcgt
     1441 cgccccctac gcagtcaaaa cctacggcat tcctgcttta tctgaacggt tggttcatcc
     1501 cgtcgtcttg ggcgatatgc ggctcaatcc gtttaccttt tccattgagc tcaccggttt
     1561 tgagattcag gagccggacc acaccccgat gatcgggttt caggaattgt ttgtagactt
     1621 tgaagccacc tcgttagttc gctcgtcata cctgttcgac gagatccgtc tcacgcttcc
     1681 gtttggcctc gtgcatctgc aggcggacgg gaagcttaat cttcttggcc tggtgccacc
     1741 cggttcccaa gaggccgagc ggcctgcacc accggtggag cagcctcgta aagaaccgat
     1801 gccgtccata gagattcggc agctgagtat tcagcgtggc gtgattgagt accgggacga
     1861 ttccaagaaa cggccggtga ccatcgatgt cgtgcccatt gaaatttcgg tacggaactt
     1921 caccagcagg gggaggggcg aaaacgccta cgcgtttacg gcggagttcg gggaaggtga
     1981 agtgctggcc tgggagggca ccttgcacct cgatccactc gaatcggatg gacatgtctc
     2041 tctctcgcat gtcggcttaa aaacgttttg gccgagtctt cgtgaccggt ttcgattcga
     2101 tatcttgagt gggacattat tggtcgatgt ccgatatcac ttcgacacac atcttgcccc
     2161 cattaacttg caggtgaccg agggcaaggt gcagttggcg gatttccggc tatctgaagc
     2221 ggggagtcga gatccggtgg tgaccgtgcc ctcgttcggc atagagggca tcaacatgga
     2281 tttgtccaag cgggagttgg gaatcggcac tgtggcattg accggagcgg aggttcgggc
     2341 gtggctcggg caggataagg tggtgaactt tcaacccctg tttgcgccgg ttgccgccac
     2401 ggcggacgct tcgccaccgt ctgagcctcc tgcgccggat gcaggtcaag cgtggaaagt
     2461 ggcggtgcag tcggttgagg tcgcgaatac ccaggtggcc tttgaagatc gaagcctcgc
     2521 gacgccggtc gaactgctgc tcgacgatgt gcatctgacc gcgggcgacg tacatgtgcc
     2581 atttaagggc gcggtgccgg tcacggccgg tttccgactc aatcagcagg gcgccttgga
     2641 agccaaggga acactccgcc tcgattccct tcaagcggcg ctggcgctca agctcgatca
     2701 tatcggtctc cggcccttcc aaccctatct ggatcggttc gttcagctcg aggtcaggga
     2761 cggtgaattg atgttggacg gagacctcgt ctatcggagc cgccacgaat cagagccaat
     2821 gttgcagtac agaggccaag ccgggctcaa caatcttcac gtagccgacc gcgcctcgac
     2881 gaaagagttt ctcgggtgga cagcgctcag cctgagcaag atggcgctgg atgtggcgcc
     2941 caccacggta aaaattggcg agattgcgtt gcgagatccg gcgttccggc tgatcaccga
     3001 taaggacggg agtaccaacc tgtcacgagt actccgtcca tccgataccc cgccggcacc
     3061 agcatcgaag cccgtggagc cggaggcccc aaagaagccc gcgacgccga ctcccgttga
     3121 gatcgctgtc gtcaggttga cgaacttgtc ggcgacgttc agcgatgaat cgatcgttcc
     3181 agctgtgagc acgggaatca cagacctgac cggcaccatc aaaggcctct cttcacggca
     3241 gatcgccaag gctgaggtgg ccttgacggg gaaagtcgac gaggttgccc cgctgaagat
     3301 ccagggccag atcaatccac tcagtgaaga cacctttacg catctgacgt ttgtgttcca
     3361 gggtgtggat ttgacgtcag tgtcacccta tgcggggaag ttcgcgggct atcctatcac
     3421 caaggggaaa ctgtcgctgg acctcatgta tcagatctcc aagaagcaat tggtcgggga
     3481 aaataaggtg ctggtcgatc agttgacgtt cggagacaaa accgacagcc cggatgccac
     3541 gacgctgccg gttcgcttgg ccgtgggtct cttgaaagac cggcggggcc ggatcgatat
     3601 cgatatgccg gtgcgaggag atttgaacga accggatttc cggtacggcc gggtcgtcct
     3661 gaacgccctg gtgaacctgc tcaccaaagt cgtcacctct ccgttttcgg ccttgggggg
     3721 actcgtcggc ggcgggggag aggacctaca gtttgtggag tttcaggcgg gaagtgacag
     3781 ccttgcggag ccagaacagc ggaagctgga gtcaattgcc aaagccttag aagagcggcc
     3841 ggcgctccgg gtagatgtca cgggagtggc tgatcccgtg cgagatcgac aggcccttgc
     3901 cctgcagaag attgcggacg aagtcctgcg gcggtttacg aagggaggga cgaaaaacct
     3961 gcaagccgtg ccctctccct cgcgggaatt cgaactgctc agtgatttgt atgcggagaa
     4021 gttgggcaag cagcccatga agcgggagga gcttcccgga ggaaaatccg ttgagcgggt
     4081 gttgacggcc gacgaactcc ggcagcagct ctttccggcc atgacggtag aggatgctga
     4141 actgcgtggt ttggcccagg agcgggcgaa agccatccgc gaacatctga tcgcgcaggg
     4201 acacttgccg gatgaacggg tatttttggt cgaggccgaa ctggcggcat cggacaagcc
     4261 gcagattcgg gtgcatctca gtttgaccgg aagctgactg atcggtgcag gctagggttt
     4321 gggctggtac cgcatccgcg tgccgtgcag gagcgacagt tcgatttcgc tggcggttaa
     4381 ttcgatgggg aagtaggcac ctgagatttt ggcggcgttg atgctggcgc cttccagctt
     4441 ggccttgcgg aaatcgatgc cgcgaaggtc cgcctgcctg aagtaacatt cgctgaagtc
     4501 caacccatcg acttccagcc cgcgcaaatc caggccgcgc aggtcgcaac gcgtgaggtc
     4561 gaccggttcc cccgccgcac gtttgacatt aaattccttg atgcacccct cgcggagtaa
     4621 ccggtacatg ggatcgttgg caatcctgag tccggtcgca ggttgtctca tgttgtccat
     4681 gcgaactcct ttggcacctg tgtcccttat cggatgcacg agcggaaact tgagagaggg
     4741 gtgattatcc gacagtgcat gtcagtaccc ttcctgcttg cgcctcgccg ctcagaaacg
     4801 gtagtcttac gccattgtcg gctttcatgt gtcagttatt tcaccacgtt tgacgggata
     4861 cctaaggagg gaaatcatgg gattacgatt gggcgacgat gccccgaatt ttacagcgga
     4921 aaccactgac gggacgatta acttccatga gtggctgggg aacgggtggg ggattttgtt
     4981 ttcccatccg aaagactata ccccggtgtg cacgacagaa ttgggctaca tggcccgcat
     5041 caagggcgaa ttcgataagc gtggcgtgaa aatccttgcc atcagcgtgg acccgctcga
     5101 ttcgcatcgc gggtggatga aggacatcaa cgagacgcaa aactgcaccg tgacctatcc
     5161 catcatcgcc gatcctgaaa agaaggtcgc caacctgtac gatatgatcc acccgaattc
     5221 gctcgacagc atgacggtgc gatccgtgtt cgtcatcggg cctgacaaaa agatcaagtt
     5281 gatgctgacc tatccggcgt cttgcggaag gaatttcgat gaattgctgc gggtggtcga
     5341 ttccctgcaa ctgactgcca agtataaagt ggcgacgccg gtcaactgga aagacgggca
     5401 ggattgcatc attaccccgg cagtgaacga tgcggaagcc aagaccttgt tccccaaggg
     5461 cttcaagtcg gtgaagccgt atctgcgcct gacaccgcaa cccaacaagt agcggcagca
     5521 gtcactgcat ttcgaacgca cagcaagagg agtcggtcga cagaccggct cctcttgacg
     5581 tttgagcgcc tgagcagctt gagtcagtac atttgaggaa gaaagacaag gggggagtcg
     5641 gcatagtacc gactccccct tgtctttgag agaggcgaat ctctgaaaac tattccggga
     5701 gcagagaatt ccgccgtgcc cacactaacg cagccaagcc gcttgcaagc aacagcacgg
     5761 cagcgggagc cggcaccgct tgcggttcgc cgtagatgaa atcatccatc gcgacaaagt
     5821 cgaagccgtc taaattcgag agggcggcat ttcccgcggt gatgcggacc cgattgatga
     5881 tggcttgctc cagtgacaca cccaggaacg acaggcttgt ggcgccgggc gtattgagca
     5941 cggtctgctt cgagagcagg ttattgttca tgtcgaagaa ctcaaccgtg gtcgggccta
     6001 agagatcgac atccgtgaag accgaaccga acccgcgaac attggccgcc tgagtggtgc
     6061 cgggcacaaa gaacttcaca tcgacaatat tgctatcgat ggcggtaaac agtttctgcg
     6121 aactgaagac ggaaaagctg gaggggaagg agggattgat gctgccgaaa cgttcgggcg
     6181 ttccgacgcc ggtgtttgcg ctgactttga atcccgaccc cggggtctcg aacacggcgc
     6241 cacgtggcga gttggagttg aagaagttgc cgggtaggtg attcgggtca gagaaaccag
     6301 caggcactcc atcccaatta atctcgcggc gtccgccggt tgccggggca ttagccaggt
     6361 tatttgtccc gagatcagcc cgaaatccat cgacagaacc ttgaatcgac gcggcattcg
     6421 agccggagga tgaatagacg gcgatggctg aagccgaaag tggcgcggcg cagacgatgc
     6481 cggtcgccat caaaagggtt agcaatcgag gagtcatcat ggatacgtgt cctccgtaaa
     6541 atatgtgata cgtagagaac acatgtagtt gagatacgta tgaatgtcaa cgcgtaattc
     6601 gcatttcgtg acacgctaca tcaaaatact aatacgtact acacggatgc tcagattccg
     6661 tatcagcatc gaacacctgt ctcagaatga tgcagcgagt gctccgtggt aggaggcgtc
     6721 gccgttctga ttgtgaataa acaggtaggg agtggaccgc gaatgcgaat ggcttcgact
     6781 cagaccagga gcagcccagc ctggcgagcg ttgtgccagg cagcagtcct ggcaaaccga
     6841 tcaaatccgc gtgcgccggt gtgggggaac cgctccctcg cctcggcgaa gcgggggtag
     6901 tcgcggtctg tgcgatgcgg agagggactg gcgttctgca atagatccat gagccagtcg
     6961 acctgatctg aggagacact gatcgagaag tcttccatgc gggtcggcag aataatcttc
     7021 acgcgcgtgg tgccctttgc cgccacgacg ggccgtccac cgatccagac gcaccgccgc
     7081 tcggcggtca atggttcagg gacggcgtcg gccagggccc gtgtcagcca tgttttcgcc
     7141 acgcgaggag cttgtacggc ttgtggaaac cattgttgga ccggcacggt cagtcccgcc
     7201 tgttcgatgt aattcaggat agctgtccgg aggccttcgc caagccatgg cgggcatgag
     7261 gcatgcgggt cggcatggcg aagatcgttc tcggcaaacc cttcgaatgt tggcccgagg
     7321 atggtgatac cctcagcggc gggattgagt cccacggggc tgtgggcggt ggcggtaaaa
     7381 cggtgccaga atgccgactg caacaaattg tttgcaaata attgccggac acgctcgaga
     7441 gagtctaccg tgtcggcgat gctctcaccc ggcaggccgt acataagata cgcgtggacc
     7501 agaatgccgg catggcggaa tgccgcggcg actctggccg tttggtctac ggtaatcccc
     7561 tttttcattt gccggagtaa acggtctgag gctgcttcaa gtccggccgt gagggctatg
     7621 cagccggaag ccgccaacag tcttgccagg tccggtgaaa atgcttcctc gaagcgaatg
     7681 tttccccacc aggaaatggt ctgattcgtt tcgagcaagc gaagtgcgag cgctttcagt
     7741 gcagccggcg gcgcggcttc gtcgacgaag tgaaaaccgc gctggcctgt ctctcggatc
     7801 agggcctcga tctgcctgat caggacatcg gtgggcgtca tctcatagcg tgcaatgtag
     7861 ttcagcccca catcgcagaa cgtgcattgt ttccaataac agccgtgcgc gacggtgagt
     7921 ttgttccaat ggccttccga ccagagccgg tgcatgggat tgaggctgtc gaggatcgag
     7981 agataacggc cgagcgggag tccgttgtag gtcggcgtcc cgagttcgtg catggatagg
     8041 tccggctccg tattgccgtt ttgaaataca acctgcccgt cggccagcac aaacgttcgg
     8101 cagagggtag cttgtgaccg cccgcctgcc aggaagtcga gcaggcagag aaatgggcgt
     8161 tcgccgtcat ccagcgtgac gtagtccacg tatcgaaaca gtcgtggatc cttgagacgc
     8221 cggagctctg tgttcacata acccccgccc aggacaacgg cagtcgcagg ccgctgtgcc
     8281 ttgatggctt gagcaatccg caaggcaccg aagagattgc cgggaaacgg cacggtgaga
     8341 cccacgacat ccggcgaact gccggccaga aggggcgcga gcgcctcgag caggagttcg
     8401 tcggtaaagc tcagtggttc ggccagagcc ttttctatcc cgtcgaagga ggacgtcgct
     8461 tgcatgatct gttcagcgta acgattcagg aataaatggg gcgcgatggt ttcgtagagg
     8521 aggtccgtca aatcttccag gtacagcgtc gcgcggtgac gggcgctgtc ggttggagaa
     8581 gtggggaaag ggctcgatcc cggtcgcccg tcggcgccga agcgaggacc ttccggcagg
     8641 aatcccgggc gagcgagatg cggggccagc atcggatcct ttccttgaag aaaggcgatg
     8701 accgggtcga tggtggccag atagctgtct tcgagcgcca ggaattgctg cacctctccg
     8761 cgccaaggtc ttcggctggc cttgatgcgg tgaaacgcct cctgaagtcc aactcgcgag
     8821 aagagacgaa ggatcatctc gagaccgaga tcagcctgct ccgcctcata ccctcgcgat
     8881 cgcaggaaac ctgtgagata ggccgttgcg ggatacggcg tgttcaattg tgtcaacggg
     8941 ggaatgatca ggagcacacg gggtagggcc atgccaggga gataccatac tgcaccgcgc
     9001 cgctgccagt gatcaggctt ggcaatcctc agtgcatcag gcggtgcgca tggtggcctg
     9061 gatgaaatac ccggtccacc gttttagccg gaagaattcg acgatcgggc gggggagttc
     9121 ctcgcgggga acggcacgga gaatgctgag cgtttggtct ctcagcagat cgattgtatg
     9181 gggttcctga cccggagtcg gatgatacat caataccaac ggcttcatgc gattcttgaa
     9241 gttgatgttc aggacgaggc cgaatgaccc atcggacaag gacacgacgg tccccggggg
     9301 atagacgccc aacgtgtgga tcagggccac cacgacctcg gtcgaaaaca gctgtttttc
     9361 atgcttgtac atggtcgcca gcgcctcggc cggtgacagc gcctcttcgg cgtagagact
     9421 gttcaccagc gtatcgtagt gatcgacggc gctgacgatt ctcgtcggga atgaaatctg
     9481 gtcccctttc aatttttcag gatagccgga tccatccaga cgttcatggt ggctgcggat
     9541 cacgtccagc acctcttcgg gaattccagg aaaccctcgc agcatctcga cgccgagata
     9601 gggatggagc tggtatttga catgttccgt ttcggaccgt cgcgcgcgtg tatccaaaat
     9661 atggcgcggg atacgccgct cgccgatgtc gagcaagagg ccagcgaggc cgatcatctg
     9721 tagcgcgtcc tggtcgatgt caaattgctg ggcgaccatc atggagaggg tggcgacatt
     9781 cagagagtgg agcacacccg catcgccgat ttcttcgggg tcgaaggcgc tcgccacggt
     9841 gccggcggcc tgggtatggg tcaagagcgt gctgatgccg ccgatcatcg tttccgcgga
     9901 acgcatgcct tcctcagatc cgcttgtgag cgcgtccatc atgtctttac tggtcccgag
     9961 catctggcgg taggtttcag aagccaattc tacggcctga tgatagtcgc catgcgtggg
    10021 aagctcttcg tccgaggggg gaggattcgt atcggcagcg gccgctgccg gagcagatcc
    10081 cgtatccggt tccacagtcg ccgtggacgc cggatccgtt tctagcagcg ttccctctgc
    10141 gtccgggtcc ggatctgatt cccgggggta aaccaggacg gtagtgagtc caagcccttt
    10201 gatcgtggcg atctggctcg cagagctgag tttaaacgag cgtcggggga agggatgtct
    10261 gaaccagccg caattcagat caacatacat ccccacacga aggcgctcgg ggctgagcac
    10321 gagggtgtcg gacttatcga gttttctgga gtcgtcggat gttttagcta aggccatgat
    10381 tgcccaaaca cggacggagg ctcactatgc atacgtactt caggttatgg cctgtatcgg
    10441 tggccggcat agaaaacttg atgaatcaag gggatatccc tgtgaccttc ccgggtatct
    10501 tgccaatggc aatgaagacg gtcaggaaaa gatgagtgcc aagcaagttt tttaatggat
    10561 aaaacatgat gcataacaac atgttgcatg caatacattg agtagaagtg gagatgtgag
    10621 ttatggagcc acctcggctt cgagaagctg aataaattcc tggcaggcac ttcggatgcg
    10681 agtcggatct cgacgtaggt cgctgcccag gacaaagacc acatcggttc cgtagaaggc
    10741 gaccatctca tggatccgcg aggcctccat tcttcctgcg gccgtcggaa agatcggggc
    10801 acaggcgccg aggctgtttc ggcagcaggc ggcgatgccc cgacaatcct cttgcgagat
    10861 cggatactcc aatccgaagg tgggataaat tgacacatca gccccggcca acctcgggaa
    10921 aagaccgaag agcactgccg gggcgatacc ggtggctgag ttgacgaagt gactgccttg
    10981 gaagtccgga tgcgcgatca ggggtaatga ggggaatggg agacgggcaa gagtgccgag
    11041 tgcgtcaaag cctgtgagtc cgggacagag aagcagcgca cccgcgcccg cgttctgcgc
    11101 gagggaggcc cggtcgagca gcgtcggcca ggggccggaa acatgtgggg cgtagaggca
    11161 gcgcctgccg gtttgccggg aagccttggc aatggcctca gcgcacagag tcacacgttc
    11221 ctcgaatcgg cagaaaggat ggtcaccgag gctttgatca tccttaatga ggtccacttc
    11281 gcccagtgcg aattcgtaag ccaggcttgc cagcgcgcgg gcagacaggc cgagtggctt
    11341 cagtacggcg cagaccaagg ggcgcacagg cacttcaagt agcgcgcgaa gtttcagtga
    11401 tccgaagcgg gggccagcca tctgggcgat cgcctcatcc ggaagcgaca gattggccag
    11461 gcgaacccgg ctttgcaggc ccgccatgcc aaagctcatg tgctggagag tgcccatggt
    11521 tccatcgagg agttcaatcg gaaatgtgag cgtcgcgcgg tgaacgccgt ctccgaccct
    11581 gtcgatgctg agcatctgac cgagcagctg atcgcgaatc acacccgacg gaatcacgtg
    11641 gtccgcggct tcgacggtct gatcgatgca gaggacagcg gcgcgccggc acgcctcctc
    11701 tttcgggccc tgaatgtgat attccacaga aaaacgttgc ccggaaaaat ggagctgctg
    11761 atccgacata ccggttcctt ccccgttgcg ctactgaggg gtgagagggt accgtaatcg
    11821 cgccctgtac gaaagctccg ggggcactca ctcctaggac gaggaaacat ggtgataaaa
    11881 ccaagacatc tacgttggct cggattgtcg gcgttgacgc tttgtgtggt gatgcacgtg
    11941 tcggccgtaa gcgggtctca tgccgccgag ataggaccac gagtgctcga cctgacctat
    12001 ccgttcgatg agacgaccca ggtgtggccc agcaatcctc cgtttcatcg tgactccacg
    12061 cagcgaggcg gcaccaccac cgaagcgtgg tatgccaccg gacaggtggc cctgtcggag
    12121 catgcgggta cacacatgga tgcgcctatt cattttgcgc agggccaagc ggggatcgac
    12181 ggcattccgg tcgagcagtt gatgggacct gcggtggtca tcgatgtgcg cgcagcggcg
    12241 gccgatgacc gtgactatcg cctgtctctc gccgacattc atcgctggga agctgcccat
    12301 gggaccatcc cgacaggcgc catcgtgatg atgctgaccg gctggggcgc ctattggaat
    12361 gatcgccaac gatatttcgg cagcgcgacg ccggagattc caaccacgct gcattttccc
    12421 ggcttctcca aggaagtggc ggagtttctc gtcagggaac ggcacatttc cggaatcggc
    12481 attgatacgg ccagtatcga ttacgggccc tctcaggatt ttgtcgtgca ccggatcgtc
    12541 aatgccgcag gcctctacgg gcttgagaat gtggcgggtc tcgcagaggt tcccgcatcg
    12601 ggcgccaggc tggtggcgct gccgatgaag attgcgggag gcaccggggc gccggtccgc
    12661 atcatcgcga ttcttccgtc acgggacggc aaatgacgag cagaaaaacc tcctgtcgtg
    12721 tgtatagcga cttcaactgc cccttttgtt atgcgatgca tgagcggctg tatgcgctcg
    12781 gtttcctcga acagatatcg tggcatggtg tgcagcatgc ccctcacttg cctcagccca
    12841 tggccacctg ggccggacat ttacatgcgg agttgaagca ggaggtgaaa atggtccgtc
    12901 ggctcgcgcc tgagttgccc atctcagtgc cggcaggaaa gccgaatacc agattagcca
    12961 ttacggtggc cgcgcgtgcg ctgcgcgagg atactcggcg agcaggcgag ttcatccggt
    13021 cgttgtatcg tctgttctgg gtagatggtt tggatctttc c
//
LOCUS       DAOQEW010000107        13002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1375_length_13002_cov_7.264154, whole genome shotgun sequence.
ACCESSION   DAOQEW010000107 DAOQEW010000000
VERSION     DAOQEW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13002
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1375_length_13002_cov_7.264154"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLS35_12060"
     CDS             <1..587
                     /locus_tag="PLS35_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNI19446.1"
                     /translation="HSIAKRYCNLDRSYEIEDLEQIGFLAVRKATEKWDFSRGNAQFQ
                     TFLYRYIQKAYQSEFDGRDKLVEITDKDDQYVKTLPYSTFIKEKRRLHSQGFKSRIFR
                     RLVPLATHEPPVSSDASPESLGLPDLPNTDEVLAFTAIDRFIEFSRKRARTIPQPTAL
                     DHNLLTESEQTITRFSPDSGSSVRHARKRRNYAN"
     gene            1103..2197
                     /locus_tag="PLS35_12065"
     CDS             1103..2197
                     /locus_tag="PLS35_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNI19447.1"
                     /translation="MQIIMIPISQIRVHEEYATLFPPLTPTERESLKVSIQEAGIHDP
                     LRVERQPTDIASAQPYVVLAGHHRLSIAQELNIPEVPCIVVTTLELKVAALFDNIHRR
                     QLSDTLRQQMQDEERRYRTKLRERLIPEILDVLPMLPPEVQLQLTQSNHDTQRDFISK
                     WMAQIQQLSIQRFPEPQNNKLAAEEPDTSETPAPKGHKHLIRQINQLTKDFERQVAQQ
                     KKELEEVRQHNVSLSRQLRAQTDEKEKLHEELQSLQRQLTSDDLDHKAERILKLQRKG
                     RSQSLTIDEAGPLISGLLHTRKTIQLLQAFAQRIATLTTPDQTTILEHIKFTSQALGE
                     LEQVLPSPTASPLHTPRQTLSVHKGLSADT"
     gene            2318..2932
                     /locus_tag="PLS35_12070"
     CDS             2318..2932
                     /locus_tag="PLS35_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19448.1"
                     /translation="MQCSSPESFASVDHAQPGEDHRSVLRTTLSKITLYNFAAAHSLD
                     TPIELTERLLLCILLANEDRHLSRTELHQITKINPSTIDSALLNLEQRNEVHTLPQHA
                     PEQTSYELTASGHTVAISLESWTTSFYQYAECGSDAGVRDLLHQLQQAIYSLIATNTI
                     PTTRACIACDFFAPESQTGGYCRFLSTPLTIQTTRPTASDTRTP"
     gene            3007..5118
                     /locus_tag="PLS35_12075"
     CDS             3007..5118
                     /locus_tag="PLS35_12075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI19449.1"
                     /translation="MSIHDANTTSVVPLPLDPSLPMEDPSEPSSHTLPSQTLPDWLHA
                     RIQTPDNITQERQITVSLDLATISPLIQNAQLNPEQLAVVTAPAEPTLVLAGAGSGKT
                     HTLIHRVAHLVTQGLPPDQILLITFTRKAAEEMIHRTQLLLQQHSLKIQGGTFHAMGL
                     IWLKHYGGALGLPPKFSIMDEDDAQGLVHLLMSKLSFSRRKGFPKKQHILALFSRAVN
                     TLRSIADTVSSDFPWLETHTPQLVVLFRAYTETKWRQHRVDYDDLLFLVYKLLCLHEP
                     TRRRLGTHYQAILVDEYQDTTKLQSEIIRLLGSHHHNVMVVGDDAQSIYGFRGAHTKN
                     MLEFQDVFSDVRLYKLEHNYRSTAPILTLANTVLHDAKALYPKRLFTTKHGGQPPELI
                     ACYSEQAQSDLLTERILQCRDEGIPFHRMAVLFRSSAHSNNLELTLARHKLAFVKYGG
                     LQLTETAHFKDVLAYLRAADNPLDSNAWHRLLLLLDRVGPKLAERLIATMQEAPHPLD
                     VLDHVPGKAESAIRLLNNVIRTTQDDTISLADRLTFVIKHYEPILKRDYPEDAKVRLE
                     DLKQLCYLARQSLNLGAFLEDLTLNPSTTPGTKHAHPEHHLVLSTIHSAKGLEWDVVF
                     VIWVLDGQFPPVRSWHDEDALEEERRLLYVAITRPRNQLFLTYPSEHYHNASQQNLDS
                     LCRFLAPIPDDILPGFAMDQV"
     gene            5184..5714
                     /locus_tag="PLS35_12080"
     CDS             5184..5714
                     /locus_tag="PLS35_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19450.1"
                     /translation="MARVTWDTETKSLARGFYAIQITPVGRFGAVGECTFDAAVGDIV
                     EVKRGETKDREYRGFYLVVPKGYLILVAIADEVDDALKVVQYVAGQASPQDLAKGHWR
                     FESAASSWKGRTAPVDQPRMEPHPPRPVAQLPPIEKRQTKVTGEPASSAPRSSTTRSF
                     TRVRRHTPQPSKDEKE"
     gene            5711..6826
                     /locus_tag="PLS35_12085"
     CDS             5711..6826
                     /locus_tag="PLS35_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNI19451.1"
                     /translation="MTFLIASFHVRLHYTWLIGFGLITWSLAANYFHDTQADLPVSYQ
                     WASGAIATLLILVSVFLHELAHAVTARRLGYAVKTIELHILGGWTIFERELSSPRVEI
                     LVALAGPGCSFLLTILLYALKADPLANFLYKVNLTLGAYNLFIPCLPMDGGRILRAVL
                     WTQTHSFAVATERTAQMSKQISSGMMAIGILGIIFQYQTLWLAIIGGILRMIAETAYR
                     TVQQSEQLTRPLQEVMIPRDRMVTLAHDTPLQELQDQFLRYGYRAYPTAEHGQITGLV
                     YYKDVRTDPKWLAPNGTTITPFIRNLSSDLIVHPVHTLQRALDAMLLSGSDQLLVFAG
                     GTCVGMVTRSMIARVQDAIGDPDSGPTLTRQREGFPI"
     gene            complement(6795..8204)
                     /locus_tag="PLS35_12090"
     CDS             complement(6795..8204)
                     /locus_tag="PLS35_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HNI19452.1"
                     /translation="MSEQGIEELRIRNRAGQRDPIVDGKVLVGYGACLQDPQRREGYL
                     VIRDEDRSGHVGIFGTTRIGKTRLMESILEQDIRSHKSVVAIDPKGDIDLFSKVVQVA
                     AEAGRLDEVMLVTPIFPDYSNYLDPLANYYMEDELVSHIVSGIKVKDDYYLSIASEVS
                     QVIVSGLIKLATSRGQVPTISFNDVNQRVGYTELINFRDVLKVLNDTEELCGLIDRVC
                     HGPGIAEHFAKVSSSLRTMLSALTFGNIGRIIGKARTNEFITRLEQGKRVILVCHTGS
                     LLSPRASLIFGRVFVSMIQSLVGRLFASGRQLDPPLCVHIDEGHNVLYPGIQEIFNKA
                     GGANVWLHFYEQSGAQMSAEIGADLARSISDNINSWIYFRVNHAETAQSVEDASPLVQ
                     RPRSVVSVQGNVTLHLNEERLVLRDRVLHLPKRCFYMRTHGQWYKGKTLDSSASYVQV
                     KFPTPAVNSPKSETPPSAA"
     gene            8364..9074
                     /locus_tag="PLS35_12095"
     CDS             8364..9074
                     /locus_tag="PLS35_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19453.1"
                     /translation="MEITTKMLAQPRDNQDADRHALTPEPRQLVTARGTHRNLPNRRT
                     GYTQKATIGGHKLYIRTGEYEDGTLGEIFLDMHKEGAAFRSLMNCFAIAISLGLQYGV
                     PLEEFVESFTLMRFEPNGPVSGHDQIKMASSILDYIMRDLAINYLDRQDLAHVQVTHD
                     DLRGDSVKPYAKSTNHQTRQESSSLNTMASHPTNDIEEARIKGYEGDPCPECHRFTLV
                     RRGTCRTCSTCGSSTGCS"
     gene            9119..9340
                     /locus_tag="PLS35_12100"
     CDS             9119..9340
                     /locus_tag="PLS35_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19454.1"
                     /translation="MSVPLPTPPHNIWLQRFKRLAQLTASLNSEDPRFPIIMTLLDKC
                     DRDYRTDNHASFIETGKRIRSLMDTPITS"
     gene            9433..10986
                     /locus_tag="PLS35_12105"
     CDS             9433..10986
                     /locus_tag="PLS35_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI19455.1"
                     /translation="MSEQYPLAIDFRTARNVSVPLIAVNSLDPAATMRALAWSLVNVA
                     SASQADTDNEFGLHAFPILKWDIGHGLIPLNEEGKNWHTQNVGPSKQNTTINPAEALR
                     VLEKVPQRTLTFILNGHRYLSNDFFIQGLWNCRDLYKTNGSTIVLLGIGFKLPPELAN
                     DVVLLNEPLPTATQLESIIKEQLSAASLPLPDPATLAKAVDATLGLGAFTAEQEVARS
                     MQASGLNVDKLWERKRQIIDATPGLSVWRGGSSYAQIGGVATIKHFLKQVLVSKRAPR
                     CIVWLDEIEKSLSGSTGQASDTSGVSQALLGILLSYMQDHQASGLLLVGPNGTSKSAI
                     AKATGAEANIPTIALDISGLKSSLVGSSEQQMRQALNVISAISQDKACFIATSNNISQ
                     LPPELIRRFGYGTWYVDLPSQDEREAIWNIYFAKFGLAADTDRPNDHNWTGAEIERCA
                     RLSWELSMPLSEAAKYIVPTAISAKESIKALEAQAHQTYLSANREGVFDQNRDTPHHT
                     RPRTITLAQ"
     gene            11021..11338
                     /locus_tag="PLS35_12110"
     CDS             11021..11338
                     /locus_tag="PLS35_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19456.1"
                     /translation="MPCYLEQRVSVELNMADVPLLISAGSSLGWEHTLNDDVLVFTTP
                     QNQQVSISKGRATLDQRHEALILELKQAYAQTIIQTAAEQFGWTLERVEGAEQELLLT
                     RFS"
     gene            11360..11563
                     /locus_tag="PLS35_12115"
     CDS             11360..11563
                     /locus_tag="PLS35_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19457.1"
                     /translation="MQTDSIRIKIEPDGTIKLITDEISLANHATAEDVIQQIITLSGG
                     SLQSSRRSTHNHHHHQADEHLHH"
     gene            11575..12486
                     /locus_tag="PLS35_12120"
     CDS             11575..12486
                     /locus_tag="PLS35_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19458.1"
                     /translation="MLNAQSAFSSLWANAFCLMLTYRKLGIHRRMDSATVLSIDTEPS
                     RVRVTKSILSCPEYLAITRLYSRVRTKLEKLTLPAGLRSGMYLIPVSLASEVDTIIAN
                     AEDELRQLVDVFLAQYDKRIVEEESRLKAAYHTRDYPDPDTVRAAFGLTYSYITFDLP
                     GTLETISATMLKREERRSMEKVNNMLEDVNTYLREAMRSLVNHLVDRLSPSESGKSKT
                     FKSSTLTNLLEFLNTFDARNLGNDIELSQLVTTAKNVLSGVDSQLLRSDQSIANVVQN
                     GMGTIKAQLDSLLIKKPIRLYSFDEAA"
     gene            12545..>13002
                     /locus_tag="PLS35_12125"
     CDS             12545..>13002
                     /locus_tag="PLS35_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19459.1"
                     /translation="MSISPDAIAHARSINLIEFLISRGHKPVSRRPHHADFHAPYRED
                     RNPSFSVSQRPDGTWVWYDFGRNEHEGCKQHGDLIDLVQLLDHVPFAEALHRILHNTV
                     RSMAAPSKPSTAPPTDKERIAHAKKLYYAAQANMTPEREEELRSYFHKLAL"
BASE COUNT         3247 a         4110 c         2971 g         2674 t
ORIGIN      
        1 tccactcgat cgcgaagcgc tactgcaatt tagatcgctc ctatgaaatt gaagatttag
       61 agcagatagg atttctggcc gtcagaaagg ccactgagaa atgggatttt tcccgtggca
      121 acgcccaatt ccaaaccttc ctctatcgct atatccagaa agcataccaa agcgaatttg
      181 atggccggga caaattggtc gaaatcacag ataaagacga ccaatacgtc aagacgctgc
      241 cctattccac attcatcaaa gaaaagcggc gcttacacag ccagggcttt aaaagtcgca
      301 tcttccgaag gcttgttcct ctggcgactc acgaaccgcc cgtctcctct gacgcctcgc
      361 ctgaatccct gggactgccc gatctcccca acaccgatga ggtgctggcg ttcacggcca
      421 tcgatcggtt catcgagttc tctcgcaaac gcgccaggac gattccgcag ccaaccgccc
      481 tcgatcacaa cctgttgacc gagtcggagc agaccatcac gcgattctct cctgacagtg
      541 gcagctccgt tcgtcatgca cggaaacgtc gaaactatgc caactgatac gacacgaagc
      601 ctcgcggaaa ccatctgccg ggaggtgggg ctccacgcag ggcctactcc gatgcccatg
      661 gagccagttc tgcccatgag cgggttcagg gagatttgcc gtgcccacaa tattccctgc
      721 agtgattgga agctctatcg gtggttaacc ttggaacgcg atagtctgcc aaggctcgtg
      781 caaaccattc ttcagcatgc accggcccag tggtacatcg ttaccacccc gtctcagggc
      841 cgctatcgag gtcggcaata tgtcgtccct ctgcactatt tgtccgcgct tcatcgcaac
      901 tttgcaatcc aacgtagact gacaaaaaac ctcgctgagc cggactccag cgctcaacaa
      961 attgccaatg gaatcctccg ccgacagccc acgatcacca gtcatcaact ccaacgggaa
     1021 cttcatcgtc gcgggcatga gctgtcccga cgtcacattt acagaatcat cgctcgtcac
     1081 agtcagcaac aaggagtccg gcatgcagat catcatgatt cccatctcgc aaatccgagt
     1141 ccatgaagaa tacgccaccc tctttccccc gctcacccca accgagcgag agagcctgaa
     1201 agttagcatc caagaggctg gaattcacga tccgctgcga gtcgaacgac aacccactga
     1261 catagcctcc gcgcaaccct atgtagtact cgcaggacac caccgcctga gcatcgcgca
     1321 ggaactcaac atccccgaag tgccctgcat cgttgtgaca accttggaac tgaaggtcgc
     1381 cgcgctgttc gataacatcc atcgacgcca attgtcggac actctccgcc aacaaatgca
     1441 ggatgaggag aggcgctacc gcaccaagct aagggaacgg ctgattccag agatcttgga
     1501 tgtgcttccc atgctgcctc ctgaagtcca actccaactc acacaatcca atcacgatac
     1561 tcaacgtgat ttcatctcaa agtggatggc ccaaatacag cagttgagca ttcagcgctt
     1621 cccggagcca cagaacaaca aactcgccgc agaagaaccg gacacttcgg aaacaccagc
     1681 tcccaagggg cacaaacacc tgatccgcca gataaatcag ctgaccaagg attttgaaag
     1741 acaggtcgca cagcagaaga aggaattaga ggaagtacga caacacaacg tttcgctttc
     1801 tcgtcaactg cgggcgcaaa ctgacgagaa agaaaagtta cacgaggagc tccagtccct
     1861 tcaacggcaa ctcacgagcg acgatctcga ccacaaggcc gagcgcattc tcaaactaca
     1921 gcgcaaaggt cgctcccaga gcctgacgat cgacgaggca ggccccctca tttctgggct
     1981 gctgcacaca cgtaaaacca tccaattgct tcaggccttt gcccagcgca ttgccacctt
     2041 gaccacccct gatcagacta cgattctcga acatatcaag ttcactagcc aggccctagg
     2101 agagctcgaa caagtgctcc cgagccccac agcctcgccg ctccacacac cgcgacaaac
     2161 gctgtcagta cacaaggggc tctctgctga tacctagtgc tacgtaagct tatggggaaa
     2221 tccccaagtc agcgccccaa tgcaacaggc atttggccac ttgtccgaga catctaatac
     2281 ttgtactact gaactgtgga aactggagaa tctacacatg cagtgttcct ctcctgaatc
     2341 gttcgcatcc gtcgatcacg cccaaccggg cgaagatcat cggtctgtgc tccgcacaac
     2401 actttccaaa atcactctgt ataattttgc cgccgcacac agtttggata caccaatcga
     2461 actgaccgaa cgactcctgt tgtgcatctt attggccaat gaagaccgac acctctcgcg
     2521 caccgagcta caccagatta ccaagatcaa cccatccacg atcgatagcg ctctcctcaa
     2581 tcttgagcaa cgcaatgaag tacatacgct accgcagcac gcccctgaac agaccagcta
     2641 cgaactcacg gcgtccggac acactgtagc tatctcactg gaatcgtgga ccaccagctt
     2701 ctaccagtat gcggaatgtg gatcagatgc cggggtacga gatctgctcc accaactcca
     2761 acaggccatc tatagcctta ttgcgaccaa cacaatccca acaactcggg catgcattgc
     2821 ttgcgacttc ttcgcgcctg agtcccaaac aggcggatac tgtcggttcc tgagcacgcc
     2881 actcacaatc cagacgacca ggcctaccgc ttctgacacc cgtactccct aacgcgacca
     2941 acgtccctcg tctatcaatt cccgaaatcc tcactccgtc caattccggt aacacatagg
     3001 tacttcatgt ctatccatga cgccaacacg acatccgtgg tcccgctccc tctcgatccg
     3061 tcgttgccga tggaggatcc gtctgaacca tcctcccaca cactcccgtc tcaaacactc
     3121 ccagactggc tgcacgcacg cattcagaca ccggataaca tcacccaaga gcgacaaata
     3181 actgtctcgc ttgatctcgc cacgatctcg cccctcattc agaatgcgca actcaatcca
     3241 gagcaactcg ccgtggtgac agccccagcc gaacccacac tggttttggc tggtgctggc
     3301 agcggtaaaa cccacacgct cattcatcgc gttgcacacc tcgtcaccca agggcttccc
     3361 cccgatcaaa tccttctcat tacgtttacg cgcaaagccg cagaagaaat gatccacagg
     3421 acgcaactcc ttctccagca acactcattg aagatccaag gcggcacctt ccacgccatg
     3481 gggttgatat ggctaaagca ctatggaggc gcactgggtc tccctcccaa gttcagcatc
     3541 atggatgaag acgacgcgca gggactggtg catttgttga tgagcaaact gtctttttcc
     3601 cgtcggaaag ggtttcccaa gaaacaacac attctggccc tctttagtcg ggccgtgaat
     3661 acgcttcgct cgattgcgga taccgtctcc agcgatttcc cttggcttga aacacacacc
     3721 cctcagctcg tcgttctgtt tcgggcctat acggaaacga aatggcgcca acatcgcgtt
     3781 gactacgacg acttgctttt cctggtgtac aagttactct gcctccatga gcctacccgt
     3841 cgcagactcg ggacacacta tcaggccatc cttgtcgacg agtaccagga cacgaccaaa
     3901 ctccaatccg agattattag gctcctgggc agtcaccatc acaacgtgat ggtcgtcggt
     3961 gacgacgccc agagtatcta tggctttcgc ggtgcccaca cgaaaaacat gttggagttt
     4021 caagacgtct tctccgacgt gcggctctac aaactcgaac acaactatcg cagcacagcc
     4081 cctatcctca ccctggccaa caccgttctc catgatgcaa aggctctcta tccgaaacga
     4141 ctcttcacca cgaaacacgg cggccagcca ccggaactaa tcgcctgtta ttcagaacag
     4201 gcgcaaagcg atttactcac ggaacgtatc ctgcaatgcc gcgacgaggg tattcccttc
     4261 caccgcatgg cggtcttatt tcgctcctcc gcccactcga acaaccttga actgacgcta
     4321 gcacggcata agcttgcctt cgtcaaatat ggtggtctcc agctaaccga aacagcacac
     4381 ttcaaagacg tgctcgcgta tctccgcgcg gcagacaatc ctctcgacag caatgcctgg
     4441 catcggctac tcctgctcct cgatcgcgtc gggccgaagc tcgccgagcg gcttatcgcc
     4501 accatgcagg aggcccccca ccctctggac gtgctcgatc atgtccctgg caaggcggaa
     4561 tcagctatcc ggcttttaaa taacgttatc cggacgacgc aggacgatac gatttcttta
     4621 gccgatcgtc tcaccttcgt aatcaaacac tacgagccga ttcttaaacg cgattatcca
     4681 gaagatgcca aagtccggct cgaagatctg aagcaactat gttatctcgc gaggcaatca
     4741 ctcaacttag gggcattctt ggaagacctc acgttgaacc cttcgaccac accgggtacc
     4801 aagcatgccc acccagaaca ccacctcgtt ctctcgacaa tccatagcgc caaaggtttg
     4861 gagtgggatg tggtctttgt tatctgggtc ctcgatgggc agtttcctcc ggtcagaagt
     4921 tggcatgacg aggacgccct tgaagaagaa cgacgcctcc tttatgtagc tattacgcga
     4981 ccgcggaacc agctcttcct cacatatccg tcagagcact atcacaacgc atctcagcag
     5041 aaccttgact cgctctgtcg gtttctcgcg ccgatcccag acgacattct tcctggattc
     5101 gcgatggatc aagtctgatg aagtctccct gcatattccg tttcgagttc cgcacccaat
     5161 gcgaacggaa ggatcgcccc tttgtggcac gcgtgacatg ggacaccgag accaaatctc
     5221 tggcacgcgg tttttacgcc attcaaatca ccccagtagg tcgttttggg gccgtcggcg
     5281 aatgcacctt cgatgccgcg gtcggggaca ttgttgaagt gaaacgcggt gaaacaaaag
     5341 accgggaata tcgcggcttc tatctcgtcg tccccaaggg atacttgatc ctcgtcgcga
     5401 ttgccgatga ggtcgatgac gccctcaaag tcgtccaata cgtggccggg caggcttcac
     5461 ctcaagacct cgcaaaggga cattggcggt tcgaatctgc cgcaagctcg tggaagggaa
     5521 gaacggcgcc cgtcgatcag ccacgaatgg aaccacatcc cccgcgacct gtggcgcagt
     5581 tgccaccgat cgagaagcga cagactaaag tcacgggaga gccagcttcc tccgcacctc
     5641 gaagcagcac cactcgttcc ttcacacggg ttcgcagaca tacaccccag ccaagcaagg
     5701 atgagaagga atgacgttcc tcatcgccag tttccatgta cggctccatt acacatggct
     5761 catcggattt ggtctcatca cctggagtct cgcagccaat tactttcacg acacgcaggc
     5821 tgacctcccc gtcagctacc agtgggcgtc cggagcaatc gccaccctcc tcattctcgt
     5881 ctcagttttt ttgcatgaac tagcccatgc cgtcaccgcc cggcgactcg ggtacgccgt
     5941 caagaccatc gaattgcaca ttctcggagg ttggacaatt tttgagcggg aactctcaag
     6001 ccctcgggtg gaaattctgg tcgccctcgc aggtccaggt tgctcctttc tccttaccat
     6061 tctcctctat gcgctgaagg cagaccctct ggcaaacttc ctctacaaag ttaatctcac
     6121 actcggcgcc tacaatctct tcattccctg cctgccaatg gacggcggcc gcatcttacg
     6181 ggcagtcttg tggactcaaa cccattcctt tgcggtcgcc actgaacgta ccgctcaaat
     6241 gagtaaacaa atcagcagcg gcatgatggc tatcggcatt ctaggaataa tctttcagta
     6301 ccaaacgcta tggcttgcga ttatcggcgg cattctccgc atgatcgccg agaccgctta
     6361 tcgcaccgta caacagagtg aacagctcac caggccgctg caggaagtga tgattccgcg
     6421 ggatcgcatg gtgacgctgg cccacgatac gcccctacaa gagctgcaag accagtttct
     6481 tcgttacggg taccgagctt accccaccgc cgaacacggg cagatcacag gcttggtgta
     6541 ttacaaagac gtgcgcaccg accccaagtg gctcgctccg aacggaacga ccatcacccc
     6601 gttcattcgc aacttgtcct cagatctaat cgtgcacccg gttcacactt tacaacgggc
     6661 gctcgatgcg atgctcctca gcggttcgga tcagctgttg gtgtttgcgg gtgggacgtg
     6721 tgtcggaatg gtgacacgct caatgatcgc ccgagtccaa gacgcaatcg gcgatccgga
     6781 ctcgggccct acgcttacgc ggcagaggga ggggtttccg atttagggct attgaccgca
     6841 ggtgtaggaa acttcacctg gacgtaggac gcactgctat ccagcgtctt ccctttgtac
     6901 cactgcccat gagtacgcat gtaaaaacac cgctttggca aatgcaacac gcggtctcgc
     6961 aacaccaagc gttcttcatt cagatgcaac gtgacgttgc cttgcacaga cacgaccgac
     7021 cgtggccgtt gcaccagcgg ggaagcatcc tcaacacttt gcgccgtctc agcatgattg
     7081 actcgaaaat agatccagct gttgatattg tcgctgatac ttcgggccag atccgcccca
     7141 atctcagccg acatttgcgc acctgattgc tcatagaagt gcagccaaac gtttgccccg
     7201 ccggccttat tgaaaatttc ttgaatcccc ggatacagca cgttatgccc ttcgtcgata
     7261 tgcacgcaca acggaggatc caactgacgg ccggacgcga aaagacgtcc aacgagactt
     7321 tgaatcatag acacaaacac ccgtccgaaa atgagggacg cgcgaggact caacaatgac
     7381 ccagtatgac ataccaggat gacccgcttg ccctgctcca gacgagtgat gaattcattg
     7441 gttcgcgcct tcccaataat ccgaccaata ttcccaaaag tcagcgcgct cagcatcgtg
     7501 cgaagggacg acgacacctt ggcgaaatgc tccgcgatgc cgggaccatg gcacactcta
     7561 tcaatcaagc cgcacagctc ctccgtatca ttcagaacct ttaagacatc tcggaaattg
     7621 atcaattcag tgtagcccac ccgttgattg acatcgttga aggagatggt cgggacttgc
     7681 cctcgacttg tcgcgagttt gattagtccc gagacgatca cctgggaaac ctcagacgca
     7741 atggagaggt agtagtcatc cttcaccttg atccctgaga cgatatggct gaccaactca
     7801 tcttccatat agtaattcgc caacggatcg agatagttgg agtagtctgg aaagatcggc
     7861 gtcaccagca tcacttcatc cagcctcccg gcctctgcag cgacctgcac gacctttgaa
     7921 aagaggtcga tgtccccttt gggatcgatt gcaaccaccg atttgtgact ccggatgtcc
     7981 tgctcgagga tggactccat caatcgcgtt ttcccgatac gggtcgtccc aaaaatcccc
     8041 acatgcccag aacgatcctc atctcggatc acaaggtatc cttctcttcg ctgagggtcc
     8101 tgcaaacatg cgccatatcc caccaagacc ttcccgtcca caatgggatc tcgctgccct
     8161 gctcgattac ggatcctcaa ttcctcaatg ccttgttccg acatggctcc ccccttctca
     8221 tcgtgtatta ccagaccatt gtctcagcca tacacaggag taggtcatat ccaagctcct
     8281 tctgccgccc acaccctcac tcatttagac atcagtaata caaagatatc gactaagtcg
     8341 aaatcagaac aacccggaga gccatggaga tcactacaaa aatgttggcc cagcccagag
     8401 ataaccaaga tgccgataga cacgccctca cccccgagcc gcgccagctc gtaacggcca
     8461 gaggaacaca tcggaacctg ccgaatcgcc ggacaggtta tacccagaaa gccacgatag
     8521 gcgggcacaa gctctatatc aggacaggag agtatgaaga cggaactctg ggggaaatct
     8581 tcctggacat gcacaaagaa ggtgccgcat ttcgtagtct catgaactgc tttgcaatcg
     8641 cgatatcctt aggactgcaa tacggcgttc cgctcgaaga attcgttgaa tccttcacgt
     8701 tgatgcgctt cgaacctaac ggaccggtat cgggacacga tcaaatcaag atggcctcgt
     8761 ccatcctcga ctacatcatg cgtgatctgg cgatcaacta cctcgacaga caggacctcg
     8821 ctcatgtcca ggttactcat gacgacctcc ggggagactc cgtcaaaccc tatgccaaat
     8881 ccacaaacca ccaaacacgg caagaatctt catcgctcaa caccatggcc tcgcacccaa
     8941 caaacgacat tgaagaagcc cgaatcaaag gctacgaggg cgacccgtgc ccagagtgtc
     9001 atcgattcac tctcgtccga cggggcacgt gtcgaacatg ctcaacatgc gggagctcca
     9061 cgggttgcag ctgaacctag ctaagacagt ccctttactc agatcacacg gagtgtctat
     9121 gtcagtccca ctgcccaccc ctccacacaa catctggctc caacggttca aacgccttgc
     9181 tcagttgacc gcctcgctca attccgaaga cccccgcttc cccatcatca tgacactact
     9241 cgataagtgt gatcgcgact accggacaga caaccatgcc tcgtttatcg agaccggcaa
     9301 acgcattcgt tccctcatgg acaccccaat caccagctaa tacctacccc agaaacgacc
     9361 gcgccaccag cgtgcctcta acaccatttc atggatcacg acctcttacc ctccgaaccc
     9421 aaaaggatcc acatgtcaga acaatacccg ctggccatcg attttcgtac tgccagaaac
     9481 gtcagtgtgc cgcttattgc cgtcaactca ctggacccag ccgccaccat gcgagccctt
     9541 gcctggtcat tggtcaatgt cgcaagcgcc tcccaagctg ataccgacaa cgaattcggc
     9601 ctccatgctt tcccaatact aaaatgggat attgggcatg gcctgatccc gctcaatgag
     9661 gagggtaaga actggcatac ccaaaatgtc gggccctcca agcaaaatac gaccatcaac
     9721 cctgcagaag ccctccgcgt cctcgaaaag gtgccgcaac ggacactgac attcatcctg
     9781 aacggacacc gctacctctc aaacgatttc ttcattcaag gcctctggaa ttgccgcgac
     9841 ctctacaaga cgaacggaag tacgatcgtc ctcctgggta tcggatttaa actgccaccg
     9901 gagctcgcca atgacgtggt gctcctaaac gagccgctcc cgacggccac acagctggaa
     9961 tccattatca aagagcagct ctctgcagca tcgctgccat tacccgatcc tgccacattg
    10021 gccaaggccg tcgatgccac gcttggcctt ggggccttca ccgccgaaca ggaagtcgct
    10081 cggtccatgc aagcgagcgg acttaacgtg gacaaattgt gggaacgcaa gcgacagatc
    10141 atcgatgcga caccaggact cagcgtctgg cgaggaggga gcagctacgc gcaaatcggg
    10201 ggagtcgcta ccatcaaaca cttcctcaaa caggttttgg tctccaaacg cgctccccgc
    10261 tgcatcgtct ggctagacga gattgaaaaa agcctctccg gatccactgg acaagcctca
    10321 gatacatcag gcgtctctca ggccttactc ggcattcttc tgagctacat gcaggaccac
    10381 caggcctcag gtttactcct cgtcggcccc aacggcacct cgaagagtgc cattgcgaaa
    10441 gccacgggag ccgaggcgaa catccccacc atcgccttag acatctctgg tctcaagtcc
    10501 tcactggtag gctcaagtga acaacagatg cggcaagcgc tgaatgtcat ctcagctatc
    10561 tctcaggaca aagcctgctt tatcgccacg tccaacaaca tcagtcagct accgcccgaa
    10621 ctcatccgtc ggttcggcta tggcacctgg tatgtggatc tccccagtca ggatgagcgc
    10681 gaggcgatct ggaacatcta cttcgccaag ttcggtctcg ccgccgacac agaccgaccg
    10741 aatgatcaca actggacagg cgcagaaatc gagcgctgcg ctcggctcag ttgggaactc
    10801 tctatgccac tgtccgaggc agccaagtac atcgttccca cggccatcag tgcaaaggaa
    10861 agcatcaagg cactcgaagc acaggcgcat cagacttatc tgagtgcgaa ccgtgagggc
    10921 gtttttgacc agaaccgaga cacgccgcat cacacacgtc ctcgcaccat taccctggcg
    10981 caataagcca gcgctgtcgc ctagagaaaa ggaacacgcc atgccctgct atctcgaaca
    11041 aagagtctcg gtggagttga atatggccga tgtccccctc ctcatctccg cgggatcttc
    11101 tctcgggtgg gaacacaccc tcaacgacga cgtcctggtc ttcaccactc cacaaaatca
    11161 gcaagtctcc atttcgaaag gaagagccac gctggaccag cgacacgaag ccctgattct
    11221 ggagctcaag caagcctatg cacaaaccat cattcagacg gccgctgaac aattcgggtg
    11281 gacgttagaa cgcgttgagg gggcagaaca agaattactg ctcactcgtt tctcctaacc
    11341 cggctggagt gacctttcca tgcagaccga cagcatacgt atcaagattg aaccagatgg
    11401 aaccatcaaa ctgattaccg acgagatcag cctagccaac cacgccactg cagaagacgt
    11461 tatacaacag atcatcacac tctcaggcgg ctcactccaa agcagtcggc gctcaacaca
    11521 caatcaccac catcatcagg ccgacgaaca tcttcaccac taagcgaggt catcatgctc
    11581 aatgctcaat cggcatttag ttcactgtgg gccaacgcat tctgcctcat gctcacgtat
    11641 cgaaaactcg gcattcaccg acggatggac agcgccacgg tactctccat tgatacggaa
    11701 cccagtcgtg tccgtgtgac taaatccatt ctgtcctgtc cggaatacct ggccattacg
    11761 agactgtact cccgcgtccg caccaaattg gaaaagttga ctctgccggc cggccttcgt
    11821 tcaggcatgt acctcattcc tgtctcgctc gcctcagagg tcgacaccat tattgcaaac
    11881 gcagaagacg agctacgaca actcgttgat gtctttctcg cgcaatacga taaacgtatt
    11941 gtggaagagg agtctcgcct caaggcggcc tatcacacca gagattaccc cgatccagac
    12001 actgtgcggg cggcgttcgg actcacctac tcctatatta cgtttgatct tcccggcacc
    12061 ctagagacta tcagtgccac gatgcttaaa cgagaagagc gccggtcaat ggaaaaggtg
    12121 aacaatatgc tcgaagacgt aaatacctac ctccgagaag ccatgcgctc gctcgttaac
    12181 cacctggtcg atcggttaag tccgagtgaa tctggcaagt ccaagacctt caagagcagc
    12241 acgctcacca atctcctgga attcctgaac accttcgacg cgcgcaattt gggtaatgat
    12301 atcgagctct ctcaactcgt cacaacggcc aaaaacgtgc tgagtggagt agactcgcaa
    12361 ttgctccgca gtgatcaatc gatcgctaac gtcgtgcaaa acgggatggg tacgattaag
    12421 gcccaactgg actccctcct catcaagaag ccgatacgcc tctacagctt cgacgaagcg
    12481 gcataacctt acgaactaga cccctcaccg cgacacaccc tctttctcgg tactgataac
    12541 gcacatgtcc atttctccag acgccatcgc ccacgcccgc agtatcaacc tcattgagtt
    12601 tctcatctct cggggccaca aaccggtgtc gagaaggccg caccatgcag attttcacgc
    12661 cccttatcgt gaggatagaa atccttcgtt ctctgtctcg cagcgaccgg atggcacctg
    12721 ggtgtggtat gacttcgggc gcaacgaaca tgaggggtgc aaacagcacg gagacctcat
    12781 cgacctcgtc caactcctcg accatgtgcc gttcgcagaa gccctgcacc ggattctgca
    12841 caacaccgtc aggagtatgg cggccccgag caaaccctcc acggcccctc caaccgacaa
    12901 agaacgtatt gctcacgcca agaagctcta ctatgccgcc caagccaaca tgaccccgga
    12961 acgtgaggaa gagctccggt cgtatttcca taaattggcg ct
//
LOCUS       DAOQEW010000108        12902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1386_length_12902_cov_7.614073, whole genome shotgun sequence.
ACCESSION   DAOQEW010000108 DAOQEW010000000
VERSION     DAOQEW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12902
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_12902_cov_7.614073"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..698)
                     /gene="msrB"
                     /locus_tag="PLS35_12130"
     CDS             complement(279..698)
                     /gene="msrB"
                     /locus_tag="PLS35_12130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNI19460.1"
                     /translation="MPSNIEIGPIVKVNKTDEEWKKLLPAAAYRVLRHEDTERAFTSP
                     LHDNHQAGIYHCAGCDLAAYSSDHKFDSGTGWPSFWQPVDPKVVETRTDTKFFMTRVE
                     VHCARCGGHQGHIFEDGPKPTGLRYCINGVSLKFVPA"
     gene            complement(881..3277)
                     /gene="lon"
                     /locus_tag="PLS35_12135"
     CDS             complement(881..3277)
                     /gene="lon"
                     /locus_tag="PLS35_12135"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNI19461.1"
                     /translation="MTDANGPILTNLPILPLKRTVLFPGTMMPLTVGRDRSIAAVEAA
                     LKTEDKTLLVVAQRDAQTDQPTLEDLYTIGTKAVIKQTARTPEGHYNILIQGLERFVL
                     LKLDQVDPYLQARVKQLPPPSERSTEVEALHRAILDIITELPKLIQTPGVHEAVAALG
                     TEEDPVVLAYRIASLLNLTLDGEQQLLEASTRADLLRGLYAALSREVQILQLRDKITS
                     EAREKLGKTQREYLLREQLKTIQQELGESNGEEDEVAALRKKVQDADLPEHVRKETDR
                     ELTRLAKTPSASPEHQVIRSYLELVLELPWNKSSEEGLDLAHVRKILNEDHYGIKEVK
                     ERIVEHLAVLKLNPSAKAPILCLVGPPGVGKTSLGQSIAKSMGRTFERFSLGGLHDEG
                     ELRGHRRTYVGALPGRIIQAMRRAGVNNPVIMLDEVDKLGRDFRGDPAAALLEILDPA
                     QNHTFRDHYLDLPFDLSKVFFITTANTLETLSQPLLDRMEIIRVNGYSEREKREIALR
                     YLWPRRLKEAGLKAEEVNLPDSVLDHIIGRYTRESGVRQLEQMLGRITRKVAVTFADR
                     PSDSPAAPVEITQSLLDEWLGQERFQPEEARKNLPAGVATGMAWTPTGGDVLYIETTL
                     LPGSHELTLTGQLGDVMQESARAARSYLWSHAESMGLDISRFKRNGVHIHVPSGAIPK
                     DGPSAGITMATALASAYVGKPVRSDTAMTGEISLTGLVLPVGGIKEKVLAAHRAGIRR
                     IILPKANEKDLKEVPQEVRDELTVIPVERIEEVLPAAFNQDAASSPEKHEPMATSTAS
                     "
     gene            complement(3415..5544)
                     /locus_tag="PLS35_12140"
     CDS             complement(3415..5544)
                     /locus_tag="PLS35_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19462.1"
                     /translation="MTFAPGLMTACATQESFHSRTVPPLNNRRLPATTDPKIPLRRHL
                     PLFLFLSLSMLLNGCTQLSKLFAVTPSPDQQVDRHQQAIVSRLAPVIEAELQDNLNVM
                     LPTVRKSLADQTNKVAKFKRDLVLRSLSDPWAGLTSIEQTSLVAATAAESGIEELPAI
                     IDILEEGMERSGAPFKPLNFPATLAPEDILGFIMEALEDAHQDREQALRHLSPQDRDF
                     LFTQARPLAEQFLPQIIPPARLAEAEAAGRYAALLMQQVDYASLIAAAQRLARLGNRK
                     LLQQLDIALHHRKPVTQTVPGITGDLLLVQQTSYGLLVIGGHGPNTYDLDKGAALIID
                     LGGNDTYRGLIGASPNSDLGNSVVIDLSGNDLYQPAPLGLATGRAGVGILIDHSGDDT
                     YRLAPGSGGAGFAGLGILYDGEGQDVYEGSRFTQGAAFGGLGLLVDRAGDDRYTSFGY
                     ALGFGGPLGVGALIDVSGNDSYECGGKYPSAYNEADAPHARPQDPAFQYDCFGLGTGA
                     GLRIYSKQSAQRGQSLAGGWGLLVDLAGRDRYRSANFSQGHGYFFGLGVKLDLAGDDE
                     HQAARYGIGTAAHFGVGLSLDYQGKDRYGSKGPFYNGGTAWDGSVALAVDGGPDSDLY
                     DLPASTGLGMADLGGLGLFIEEGGADQYFIGRGLGQGADTSIGAFFDLEGRDDYTSVP
                     LPNGDARPERLNRKTYLENTGSLFVDR"
     gene            complement(5465..6991)
                     /locus_tag="PLS35_12145"
     CDS             complement(5465..6991)
                     /locus_tag="PLS35_12145"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNI19463.1"
                     /translation="MSAAHAPLEAPQQEHDLSLSRLIVVSNREPYEHRLVKDHLIWER
                     TSGGLISALDPMMRRLGGTWIAWGSGKADRDVVDEDMVVEVPPDAPTYRLRRVWLENG
                     EVKGGYQGYANQVLWPLCHLTLDRVAYRKAFWHAYQAMNARFADVVLGELAEKPGFVW
                     VHDFHLALLPGLIKASLPNQPVAIFWHTPWPGPDAFRILPERRELLESLLAADLVMFQ
                     TESFLHCFLECAKEFLGTDVLSDEHHIDYKGHRTRLVVRPISINYQSWAERAQSPQVT
                     RAMDVLRNLHIFQPTVRIGLGVDRLDYTKGLLKRLWAIDAFFQQYPQYRGHFTFIQIA
                     VPTRGDVEAYRSYRELIRETVNEINMRYGTISHPGSPQASRWRPIEFREGRIGMDTLA
                     AYYRMADLALVSSVYDGMNLVAKEYVASQVEEKGVLLVSHLAGAAEELTDAVVINPYD
                     PEGVADSIRQALEMPFGERRERMRRMRAYLEAHDIRAWADDCLRDAGILPQQDSPALE
                     "
     gene            complement(7105..8298)
                     /locus_tag="PLS35_12150"
     CDS             complement(7105..8298)
                     /locus_tag="PLS35_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNI19464.1"
                     /translation="MDSTIQAFFAPCPRGLEAVLAEELTMLGATAVKATAGGVAFRGT
                     LALCYRVNLQSRIASRILLRIAEGAYRDEHDVYDAAYAIRWQDWFTPQQRIKVKVSAH
                     RCPLKSLDFVTLRVKDAVCDRFQYARGKRPSVDTHAPDILIAVYLDGTQCTFYLDTSG
                     DPLFKRGWRKSSGEAPIRENLAAGILRLSKWTPDVLLYDPMCGSGTFVVEAALMAGRI
                     APGIGRGFAFEKLLSCDAPMLNDLRAELQAAEIPVKPGLIHAADQSAQAITSIRANLA
                     LAGLTEAVTLQQGDVLQLPAPAEAGLLVTNPPYGHRMGDDETLKTFYPQFGDHLKKHF
                     CGWTVQIFTADMKLPGLLRLAPSRRVPLFNGAIECRLFEFRMVAGSLRKPKPPAQSSP
                     GHGHH"
     gene            8333..8875
                     /locus_tag="PLS35_12155"
     CDS             8333..8875
                     /locus_tag="PLS35_12155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNI19465.1"
                     /translation="MKFCSECGAGLSKKIPPGDNLPRFVCETCQVVHYINPKIVAGCI
                     PEWEDKILLCRRAIEPRIGHWTFPAGFMEIGESTEQAAVRETFEEAHAEVEITSLYAV
                     LSLPRISQVHMIFRGTMRTPEFKPGTESLDVQLFTLDAIPWDDLAFPVIQDALERYVA
                     DVARGTFTMHFGSVFPRMKS"
     gene            complement(8944..9249)
                     /locus_tag="PLS35_12160"
     CDS             complement(8944..9249)
                     /locus_tag="PLS35_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HNI19466.1"
                     /translation="MLTLTQKAVDRLRALLLEHPEDPVVRIAVRDVNEQRLSLAITLE
                     AAVREEDVVEQIEGLTIAFDRSSAHRTGGMTVDYQAGRFSFAHPPANELGLIMPGRN"
     gene            complement(9252..>9461)
                     /locus_tag="PLS35_12165"
     CDS             complement(9252..>9461)
                     /locus_tag="PLS35_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNI19467.1"
                     /translation="MAQRLFDKTKWLASNVENLRHEPVAADLPGIHKYAVGDWRIFYS
                     IDRSEQMVDIHYIQHRPQPGRTPGA"
     assembly_gap    9462..9561
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9649..10236)
                     /locus_tag="PLS35_12170"
     CDS             complement(9649..10236)
                     /locus_tag="PLS35_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI19468.1"
                     /translation="MSLESTPSTALSPEAIAQLVQGHKQFLAFLERRVESRAVAEDIL
                     QSAFARGLERGAGVDEEKVVAWFYRVLRNAVIDHYRQRSSAERAVEAWTREFPDVQEP
                     AAELRQEICQCVSGLLETLKPEYREALQIVDLQEGKLKDLAQQSGITAENAAVRVHRA
                     RAALRRQIEKACGTCSVHGCLDCSCEASGGSTCGA"
     gene            10689..11948
                     /locus_tag="PLS35_12175"
     CDS             10689..11948
                     /locus_tag="PLS35_12175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HNI19469.1"
                     /translation="MRFIHAADLHIDSPLRGLDRYDGAPVERLRTATRSAFERLIDTA
                     ISEHVDFLLLAGDIYDCDWQDFHTGLFFRGQMVRLEREGIRVFIVQGNHDAQGVISKQ
                     LVLPPNVTVFSSRAAHTIRLDDRSVAIHGRSFPERAVDEDLVPSYPPPVPGFFNIGLL
                     HTSLTGRAGHDTYAPTDLPTLNHKGYDYWALGHVHAREVIAERPHIVFPGNLQGRHAK
                     ETGPKGCELVTVEAGRIESEFVALDVVRWNQLIVSLDGVQRLEALGEAFRHALEPVLI
                     GAMDRLHAVRVTLTGSTELHRLEASQPGTLAAAMYGAAQDIGEAEVWIEQVRLDLTTP
                     LDRVQTAQRQDAVGELVRLVDSVAGDESELQRWAQVELGDLLGSLPQEVAAGDVPKLD
                     DRVELTQLLMEAEATVLARLTDLGAEA"
     gene            11945..>12902
                     /locus_tag="PLS35_12180"
     CDS             11945..>12902
                     /locus_tag="PLS35_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI19470.1"
                     /translation="MKLLKLLLQAYGPFTGKVLDFSTGPANLHVIYGPNEAGKSSALR
                     AMMDLRFGIPVRSADDFLHAAGDLRIGGIFAAINGEAIGFVRRKGKGTTLARLNVLTE
                     QPDQSLVMEPGHERGLTGGLERAEFEAMFGLTHARLREGGQVLLRGEGDLGSALFEAS
                     AGTSGIATLLAALETDAKKLYSPHGRAQNAVINEARRRLDEQRQAWREAQTKPAEWQS
                     LYRAHEAAAATLSDVSMTLERLRRRENELIELRTVGPLLRTHDAATALLASFAGVPDL
                     PEQAREARLAALQELNHAEHSLREAEVEAERCARALGALTLEP"
BASE COUNT         2478 a         3746 c         3927 g         2651 t
ORIGIN      
        1 accggcgtgc cgatcgcgaa ttcaacctcg gcttcggtca cgatgaggca ggcattcaga
       61 ttgttcgaaa tgacgtcgct cgattgggaa cacccgagca gtgtgacgcc cagccaaagg
      121 gcggccatcg tcgagcgtag tgaagggaga gaagccatgg aattcacagt ctaggagtgt
      181 tcagcgataa attgcaaggt gcgatgagac gctcgagaga tgttcacgat cggcgacgac
      241 agcagaggtc gtcgcccaga gacatgcccg gatcgtgttc aggcaggaac gaatttcaac
      301 gagacgccgt tgatgcagta tcgcaatccc gtgggtttgg gaccgtcttc aaaaatatgg
      361 ccctggtggc cgccgcagcg cgcgcagtgt acttcgaccc gggtcatgaa aaacttggta
      421 tcggtgcgtg tctccacgac tttcgggtcg acgggttgcc agaaactagg ccaacctgtg
      481 ccgctatcga acttatggtc tgaagagtag gcggccaagt cgcagccggc gcagtgatag
      541 atgccggcct ggtggttgtc atgcagcgga ctggtaaacg ctcgttcggt atcttcgtgc
      601 cgcagcacac ggtaggccgc agccggcagc aactttttcc attcctcgtc ggtcttgttc
      661 accttcacga tcggtccgat ctcgatattc gatggcatgg atcctctcct ttgccgggtc
      721 atgtgcggtg cgcatcaacc aggtgacgtt tttcatcctg acatatgtct ttacggaagg
      781 gcaaggcgat cggatgggcg agtagtgacc ttcaacgcgt agagggagtt tgaaagcggt
      841 cgctgagcgg gccccggctt ctcagccggg gcccgagagc ttatgatgcg gtggacgtcg
      901 ccatcggttc gtgtttctcc ggcgacgagg ccgcatcctg gttgaacgcc gccggtagga
      961 cctcttcgat ccgttcgacc ggaatgaccg tcagttcatc ccgaacttct tgcggcactt
     1021 ccttcaagtc cttctcatta gccttcggca gaatgatccg ccggatgccg gcgcgatggg
     1081 cagccagcac cttctccttg attccgccga cgggaagcac cagcccggtc aggctgattt
     1141 cgcccgtcat ggccgtgtcg ctccggaccg gtttgccgac ataggccgag gccaatgctg
     1201 tcgccatcgt aataccggcc gaggggccgt ctttggggat ggcgccggag ggcacgtgaa
     1261 tgtgcacgcc gttccgtttg aaacgcgaga tgtccagtcc catgctctcc gcatgcgacc
     1321 acaggtagct gcgtgccgca cgagccgatt cctgcatcac gtctcccaat tgcccggtga
     1381 gcgtcagctc gtgactgccg ggtaggaggg tcgtttcgat gtagagcaca tcgccgcctg
     1441 tcggcgtcca ggccatgccg gtggcgacac cagccgggag attcttgcgc gcctcctccg
     1501 gctggaatcg ttcctgaccg agccattcgt ccagcagcga ttgggtgatc tcgaccggcg
     1561 cggcagggga atcggacggc cgatcggcaa aggtcaccgc cactttccgc gtaatgcgac
     1621 ctaacatctg ctccagttgc cgcacaccgg attccctggt gtaccggccg atgatatggt
     1681 cgagcacgct gtcgggcaaa ttgacttctt ctgccttcag gccagcctct ttcagccgtc
     1741 tcggccagag ataacgcagg gcgatctccc gtttttcccg ttcgctatac ccgttcaccc
     1801 ggatgatttc catgcgatcg agcaacggct gcgagagagt ctccagcgta ttggccgtcg
     1861 taatgaagaa caccttcgac aagtcgaacg gcagatcgag gtaatggtcc cggaacgtat
     1921 ggttctgcgc gggatccaga atctccagca aggccgccgc gggatcgccg cggaagtcac
     1981 gccccagctt atccacctca tccagcatga tgaccggatt gttcacaccc gcccgtcgca
     2041 tcgcttgaat aatgcgtccg ggcaaggccc cgacgtaggt ccggcggtgg ccgcgcaatt
     2101 cgccttcgtc atgcaagccg cccaggctga accgttcaaa tgtccggccc atggatttgg
     2161 caatcgattg tcccagactc gtctttccca cgccgggagg gccgacgagg cagagaatcg
     2221 gcgctttggc cgaagggttc aatttcaaca cggccaaatg ttcgacaatc cgttccttca
     2281 cttccttgat gccgtagtga tcctcgttca agatctttcg cacatgggcg aggtcgaggc
     2341 cttcttccga cgatttgttc cacggcaatt cgagtaccag ctcgagatag ctccggatga
     2401 cctgatgctc cggtgaggcg ctgggcgttt tggccaatcg cgtcaactcg cgatcggttt
     2461 cttttctcac atgctcagga agatcggcgt cctgcacttt cttcctcagt gccgccacct
     2521 cgtcttcttc gccgttgctc tcgcccaact cctgctgaat cgttttcagc tgctcgcgta
     2581 gcaggtactc gcgctgggtt ttgcccagtt tttccctggc ttcgctggtg atcttgtcgc
     2641 gcaattgcag aatctgaacc tcgcgcgaca gcgcggcgta cagcccgcgc aacagatccg
     2701 cacgggtgga cgcttccagc agttgctgtt cgccgtccag cgtgagattc aacaacgaag
     2761 cgatgcggta ggccaacacc acggggtctt cttcggtccc cagggccgcc accgcttcat
     2821 gcacgcctgg tgtctggatc agtttgggca gttcggtgat gatgtcgagg atggctcgat
     2881 gcaatgcctc gacctccgta ctccgttcgc tgggcggtgg caattgcttc actcgcgctt
     2941 gcagataggg atcaacctgg tccagcttca gcagcacgaa ccgctccagt ccctgaatca
     3001 aaatattata atgaccttcc ggggttcgag ccgtctgctt gatgacggct ttcgtcccga
     3061 tcgtatacag gtcttcgagc gtcggctgat cagtctgagc atcccgttgc gccacgacca
     3121 gcaaggtctt gtcttctgtt ttgagcgctg cttcgacggc cgcgatggaa cggtcgcgtc
     3181 ccaccgtcaa cggcatcatc gtgccgggga agaggaccgt tcgtttcaac gggagtatcg
     3241 gcaggttcgt gagtatgggg ccgttcgcat cagtcatatc gtcctccacg gttctgaggt
     3301 ggtcatcaca aggaagatga atcggctaca aggaatgatg ttcgttcccg ttgatcaccg
     3361 ttctttgaga tagccatgga ggaaggggag tcaagcgggg ggcaaaacta ccggttaacg
     3421 gtcgacgaag agactgccgg tattttccag ataggttttg cgattcagcc gttccggtcg
     3481 cgcatcaccg ttcggcagcg gaacagacgt atagtcatcc cgaccttcca agtcgaaaaa
     3541 ggcgccgatg ctcgtatctg cgccctgtcc taatccccgg ccgatgaagt actggtcggc
     3601 gccgccttct tcaatgaaca ggcccaagcc gcccagatct gccattccca ggccggtcga
     3661 agcgggcagg tcatacaaat cgctgtccgg accgccgtcc acggccaggg cgacgctgcc
     3721 atcccaggct gtgccgccgt tatagaacgg gcctttggaa ccgtaccgat ccttgccttg
     3781 atagtcgagg ctcaggccta cgccgaaatg cgcggcggtg cctatgccgt accgcgccgc
     3841 ctgatgttcg tcatcaccgg ccagatcgag cttcacgcca aggccgaaaa agtagccgtg
     3901 tccttgcgaa aaattggcgc tgcgataacg atcacgaccg gccagatcga ccagcaggcc
     3961 ccatccgccg gcgaggctct gcccccgttg cgccgactgc ttgctgtaga tgcgaagccc
     4021 tgccccggtg ccgagcccga aacaatcata ttgaaacgcc ggatcctgag ggcgagcatg
     4081 cggcgcgtcc gcttcattgt aggcgctcgg gtattttccg ccgcattcgt atgaatcatt
     4141 gccggagacg tcaatgagcg cccctacgcc caagggcccg ccgaatccta gcgcgtaccc
     4201 gaaactcgta tagcgatcat cgccagcgcg atccaccagc agacccaatc cgccgaaggc
     4261 tgcgccttgg gtaaaccggc ttccttcgta cacatcctgt ccttctccat catacaggat
     4321 gccgagccct gcgaaaccgg cgccaccgga gccaggtgcc aaccggtagg tatcgtcccc
     4381 gctatgatcg atgagaatac cgacaccggc gcgtccagtg gctaagccca gcggggcggg
     4441 ctggtagaga tcgttgccgc tcaggtcgat gacgacactg ttgcccaggt cgctgttcgg
     4501 gcttgcgccg atgaggcctc gataggtgtc gttgcccccc agatcgatga tgagcgcggc
     4561 tcccttgtcg aggtcatacg tattcgggcc atggccgcct atcacgagta agccgtagga
     4621 agtctgctga accagcagca gatcgcccgt gatgccggga accgtctggg tgaccggctt
     4681 tcgatggtgg agcgcaatgt ccagttgttg caataatttc cggttgccga gccgtgctaa
     4741 tcgttgtgcg gcggcaatga gcgatgcgta atccacctgt tgcatgagca gcgcggcata
     4801 gcggccggcg gcttcggctt ccgcaagcct ggccggcgga atgatttgcg gaaggaattg
     4861 ttccgcaagc ggacgcgcct gggtgaagag aaagtcccga tcctgcggag acaggtgccg
     4921 gagtgcctgt tcgcggtcct gatgggcatc ttcaagcgct tccatgataa aaccgagaat
     4981 atcctccggt gccagggtag ccggaaagtt gaggggcttg aacggcgcgc cgcttcgctc
     5041 catcccttct tccaaaatgt cgatgatggc gggcagttcc tcaattccag actcggcggc
     5101 ggtggcggcg acaaggctgg tttgctcgat cgaggtgagg cccgcccagg gatcagatag
     5161 cgaacgcaac accagatcgc gcttgaactt cgccaccttg ttcgtctgat cggcgaggct
     5221 cttgcgaact gtcggtagca tgacgttcag attgtcttgc agttcagctt cgatgactgg
     5281 cgcgagccgg gatactatcg cctgttggtg gcgatccact tgttgatctg gcgacggcgt
     5341 gaccgcgaac agtttggata gctgtgtgca gccgttgagg agcatcgaga ggctcaggaa
     5401 caggaacaac gggaggtgtc ggcgtaacgg gatcttcgga tcggtggttg cgggcaggcg
     5461 tcgattattc aagggcggga ctgtcctgct gtggaaggat tcctgcgtcg cgcaggcagt
     5521 catcagccca ggcgcgaatg tcatgtgcct ccagataggc gcgcatgcgt cgcatcctct
     5581 cacggcgctc tccaaatggc atctccaacg cctggcggat ggagtcggcc acaccttccg
     5641 ggtcgtaggg attgatcacc acggcgtcgg tcaattcttc tgccgcgccg gccaaatggc
     5701 tcaccagcaa cacgcctttc tcttcgacct ggctggcgac atactctttc gcgacgagat
     5761 tcatgccatc gtaaaccgaa ctcactaacg ccaggtcggc catgcggtaa taggccgcca
     5821 gggtatccat accaatgcgc ccttcacgaa attcgatcgg tcgccagcgc gaggcctgtg
     5881 gactaccggg gtgagagatc gtgccatagc gcatattgat ctcattgacc gtttcgcgga
     5941 tcagttcgcg atagctccga taggcttcca catcgccgcg cgtcggcacg gcaatctgga
     6001 tgaacgtgaa atggcctcga tactgcggat actgctggaa gaaggcgtcg atggcccaca
     6061 atcgcttcag gagacctttg gtgtagtcga gccggtcgac gccgagtccg atgcgtacgg
     6121 tcggctgaaa aatgtgtaga ttacgtaaga cgtccatggc ccgcgtgact tgcggcgatt
     6181 gtgcccgctc ggcccacgat tgataattga tgctgatggg gcgaacgacc aatcgcgtgc
     6241 ggtggccttt atagtcgatg tgatgctcgt cggacaggac atcggtgccg aggaattctt
     6301 tggcgcattc aagaaagcag tgcagaaagc tttccgtctg aaacatcacc aggtcggccg
     6361 ccagtaatga ttccaggagt tcccggcgtt cgggcaggat gcgaaacgca tccgggccgg
     6421 gccagggagt atgccagaag atcgcgaccg gttggttggg cagggaggct ttgatcaggc
     6481 ctggcagcag ggcgagatga aaatcatgca cccagacgaa gccgggtttt tcggcgagtt
     6541 caccgaggac cacgtccgca aagcgtgcat tcatggcctg ataggcatgc cagaatgctt
     6601 tccggtaagc aacgcgatcc agggtgaggt gacacagtgg ccagaggacc tgattcgcgt
     6661 acccctgata gccgcccttc acctctccgt tttccagcca gacgcggcgt aaccgatagg
     6721 tcggagcgtc gggaggaact tcgaccacca tatcctcgtc caccacatcc cgatcggctt
     6781 tgccgcttcc ccaggcaatc cacgttccgc ccagtcgtcg catcatcggg tcgagcgccg
     6841 aaatgagtcc ccccgacgtg cgttcccaaa tcagatgatc cttcaccaac cggtgttcgt
     6901 agggctcgcg gttcgaaacc acgatcaagc gcgagagtga caggtcgtgc tcctgctgcg
     6961 gagcctcaag gggcgcgtgg gctgcgctca tagagttccg catggtgtgt gacacagata
     7021 tgatggcgcg tcgaatgaca tagattgaaa ataggaggct tatgctccac tgtcaagcat
     7081 gtattcggct gcccggagaa ccggtcagtg gtggccgtgg cccggggagg attgtgctgg
     7141 tggtttgggt ttcctgagac tgccggcgac catgcggaat tcaaacaggc ggcattcgat
     7201 ggcgccattg aacaacggga ccctgcgtga aggcgccaga cgcaaaaggc cgggcagctt
     7261 catgtcggcc gtaaaaatct gaacggtcca accgcagaag tgttttttca aatggtcgcc
     7321 gaattgagga taaaatgtct tcaaggtctc atcgtctccc atgcggtggc cgtagggagg
     7381 attggtgacg agcaggccgg cctccgccgg tgcgggaagc tgcaggacat ctccctgttg
     7441 cagcgttacc gcctccgtga gtccggcgag cgcgaggttg gctctgatcg aggtgatcgc
     7501 ttgcgcgctc tggtcggcgg cgtgaatcaa cccgggcttg acgggaatct ccgcggcctg
     7561 aagttccgcc cgcaggtcat tcaacatcgg ggcgtcacaa gagagcagct tttcaaatgc
     7621 gaagccgcgg ccgatgccgg gggcgatgcg ccccgccatc aaggccgcct cgacgacaaa
     7681 cgtcccgctg ccgcacatgg ggtcgtacag caacacgtcc ggtgtccatt tggacaaacg
     7741 caggattccg gccgcgagat tttccctgat cggcgcttcg ccggaggact ttcgccatcc
     7801 gcgcttgaac agcggatcac cggaggtgtc cagatagaag gtgcattggg ttccgtctaa
     7861 gtagacggca atcaggatgt ccggcgcatg ggtatcgacg gacgggcgtt tgcccctggc
     7921 atattggaat cgatcgcaca ccgcatcctt gacccggagc gtcacaaaat ccaggctctt
     7981 cagcgggcaa cggtgcgcgc tcaccttcac cttgatgcgt tgctgcgggg taaaccaatc
     8041 ctgccagcgg atggcgtagg ctgcgtcata gacatcgtgt tcgtcgcgat aggccccttc
     8101 cgcaatgcgc agcagaatgc ggctggcgat gcggctttgg agatttaccc gataacagag
     8161 ggcgagcgtg ccgcgaaagg ctactccgcc ggcggtggcc ttgacggcgg tggcgcccag
     8221 catggtcagt tcttccgcca ggacggcttc gaggccgcgc gggcagggtg caaagaatgc
     8281 ctgaattgtg ctatccattt ctgtctttct ctcgtcgtga tcgggagccg caatgaaatt
     8341 ctgcagcgag tgtggggccg ggctgtcgaa aaaaattcct cccggcgaca acctgccgcg
     8401 gtttgtctgc gaaacctgtc aggtcgtcca ttacatcaat ccgaaaatcg tcgccggttg
     8461 tattcccgag tgggaagaca aaattctgtt gtgccgccgg gcgatcgaac ctcgcatcgg
     8521 ccactggacg tttcctgccg gcttcatgga aatcggcgaa agcacggaac aggccgccgt
     8581 tcgggaaacg ttcgaggaag cgcatgcgga agtggaaatc acctccctgt atgccgtgct
     8641 gagcctgccc cgcatcagtc aggtgcatat gatttttcgc gggaccatgc gcacgccgga
     8701 attcaaaccc ggtacggaaa gcctggatgt gcaattgttc acgctggatg ccattccgtg
     8761 ggacgacctg gcctttcccg tgatccagga cgccctggaa cgctatgttg cggatgtggc
     8821 gcgaggtacc ttcactatgc atttcggcag cgtatttccg cggatgaaat cgtgagcggc
     8881 gccaaggcat gaaacggaac acgtcaaaca cactgacgtt gtacctgtaa cggttcactc
     8941 acttcagttg cggccgggca tgatgaggcc cagctcattc gccgggggat gggcaaagct
     9001 gaagcgtccg gcctggtaat ccaccgtcat cccgcctgtc cgatgtgcgc tcgaccgatc
     9061 gaatgcgatc gtcaggcctt caatctgttc gaccacatcc tcttcgcgaa ccgccgcttc
     9121 cagtgtaatg gcaagggaga gccgctgctc attcacatcg cgcacggcaa tgcgcaccac
     9181 cgggtcttcg ggatgttcca gcaacaacgc ccgcagccgg tcgacggctt tttgcgtaag
     9241 ggtgagcatc gtcaggctcc gggagtccgg ccaggttgcg gccggtgttg aatgtagtga
     9301 atgtctacca tctgttccga tcggtcgatc gagtaaaaga tgcgccagtc gccgacggca
     9361 tatttatgga tgccgggcaa atccgccgcc acgggctcgt gccgcaaatt ctccacgttg
     9421 gaggccagcc acttcgtttt gtcgaacaac cgctgagcca tnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn ngttggttca gtcatcgtga ttcgagcata cgggggcagt
     9601 tgccgcaaat gcaagaatag cgttcggatg tcggctgctg cattgtcctc atgccccgca
     9661 agtcgatccg ccggatgcct cgcaggaaca gtcgaggcag ccatgcacgg aacaggtgcc
     9721 acaggccttt tcgatctggc gacgcagggc cgctctggcc cggtgcacgc ggacggccgc
     9781 attctcagct gtgatccctg attgttgagc caggtctttc aatttacctt cttgcagatc
     9841 cacgatttgc agggcttcgc gatattccgg tttgagcgtc tcgagcaacc ccgatacgca
     9901 ctggcagatc tcctgtcgca gttccgccgc cggttcctgg acatcgggaa attctctggt
     9961 ccaggcttcc accgcgcgct cagcgctaga tcgttggcgg tagtgatcga tcacggcgtt
    10021 gcggagcaca cgatagaacc aggcgacgac cttttcttcg tccacgccgg cgccgcgttc
    10081 taacccacgt gcaaacgcgc tttggagaat gtcttccgcc acggctctgg attcaactcg
    10141 ccgctcgagg aacgccagga actgcttatg tccttgcacc agctgcgcaa tggcctccgg
    10201 cgaaagggct gtcgatggtg tggattcgag tgacatgacg gcagcttacc aaactctctg
    10261 ttgaactgta aggttttcga acgctctccg tcagcctgtg tgacgacgca atcacgcgtt
    10321 gacctaccaa aggagaccac catggccgat gcgaagacat ccacctccca gaaaatcgcc
    10381 aagagcgggt gctgcggaac atgcgactgt tcatcctgtg catgcgggtg tcagcccaat
    10441 gcctgccgtt gcgtgcagat caactgtcaa tgtgggtgcc gcaaacaagc agccagacga
    10501 taggctggct ttgtcccgcc cgccgagtcc ggcgggcggg actcagatga cagaggagtc
    10561 acgcgccggc attgtgagga cctggccact cgcgtatcat gcgggatcgc accgcgcttg
    10621 tcctgtccat ccggatttat gatcgtagga tgcgtgggtg atcgtgaact cctctggacc
    10681 tgtacgtcat gcgctttatt catgcggccg accttcatat tgacagtccc ctgcgcgggc
    10741 tggaccgcta cgacggcgct ccggttgagc ggttgcggac ggcgacacgc agtgccttcg
    10801 agcggctgat cgatacagcg atcagtgaac acgtggactt tttgctcttg gccggggata
    10861 tctacgactg cgactggcag gattttcata ccggcctgtt ttttcgcggg cagatggttc
    10921 ggttggagcg agaggggatc cgggtcttca tcgttcaagg gaaccatgat gcgcagggtg
    10981 tcatctccaa acagctcgtc ctcccgccga atgtcaccgt attttccagt cgcgcggctc
    11041 acaccatcag gctcgacgat cgctcggtgg cgatccatgg ccgcagcttt cccgaacggg
    11101 cggtcgatga agaccttgtc ccttcttatc ctccgcctgt gccgggcttc ttcaatatcg
    11161 gtctcttgca caccagcctg acaggccgcg ccgggcatga cacctacgcg ccgacggacc
    11221 tgccgacgtt gaaccacaaa ggatacgact attgggccct ggggcatgtg catgcgcgag
    11281 aagtgatcgc cgagcggccg cacatcgtat ttcccggcaa cctgcagggc cgccatgcga
    11341 aggaaacggg gccgaagggg tgcgagttgg tgacggtgga ggcgggccga atcgaaagcg
    11401 aattcgtggc gctggatgtg gtgcgctgga atcagctgat cgtttcgctc gatggcgtac
    11461 agaggctgga ggctttggga gaggccttcc gtcatgccct ggagccggtg ctcatcggcg
    11521 caatggatcg cctgcacgcg gtacgggtaa ccctgacggg atccacggaa ctccatcgcc
    11581 tggaagcgag ccagcccggc acgttggctg cggcgatgta tggggcggca caggacattg
    11641 gcgaagccga agtctggatt gagcaggtcc ggcttgacct cactactccg cttgatcgcg
    11701 ttcaaacggc gcaacgccag gatgcggtgg gtgaactcgt ccgtctcgtc gattccgtgg
    11761 cgggtgacga atcggagttg cagcgctggg cgcaggtcga actcggcgat ctgctgggct
    11821 cgcttccgca ggaagtggcg gctggagacg tgccgaagct cgacgaccgc gtcgaattga
    11881 cacagctcct gatggaggcg gaagccacag tattggcacg gctgactgat cttggagcag
    11941 aggcatgaaa ctgctcaagc tgctgctcca ggcctacggt ccattcaccg gcaaggtgct
    12001 ggatttttcc acgggccctg ccaacctcca cgtgatttat ggtcccaatg aagcgggcaa
    12061 gtcgtcggcc ttgcgagcca tgatggattt gcgattcggc atccctgtgc gaagcgcgga
    12121 cgatttcctc catgccgccg gtgaccttcg gatcggagga attttcgccg cgatcaatgg
    12181 cgaggcgatc ggttttgtgc ggcgcaaggg caaggggacg acactggcgc ggctcaacgt
    12241 tctgacggag caaccagatc aatcgctcgt catggagccg ggccacgaac gggggttaac
    12301 cggcgggctg gagcgggcgg agttcgaggc catgttcggt ctcacccatg cgcgtttgcg
    12361 cgaaggcgga caggtgcttc tgcgcggcga gggggatttg ggatcagcgt tgttcgaagc
    12421 aagcgcaggg accagcggca tcgcgacgct gctcgcagcg ttggagaccg acgcgaagaa
    12481 gctatatagc ccgcatggac gggcgcagaa cgcggtgatc aatgaggcac gccggcggct
    12541 cgatgaacaa cggcaggcct ggcgcgaagc ccaaaccaag ccggcggagt ggcaaagctt
    12601 gtatcgcgcg cacgaggcgg cagcagcgac gctctctgac gtgagtatga ctctcgaacg
    12661 gctgcgccgg cgtgagaacg aattgatcga attgcgcaca gtcggaccgt tgttgcgcac
    12721 ccatgacgca gcgacggcct tgttggcgtc gttcgccgga gttccggact tgcccgaaca
    12781 ggcgcgggaa gcgcggttgg ctgcgttaca ggaactcaac cacgctgagc acagcctgag
    12841 agaagctgag gtcgaggcgg agcgatgcgc ccgtgcgctt ggcgcgttga ccctcgagcc
    12901 at
//
LOCUS       DAOQEW010000109        12744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1416_length_12744_cov_8.541099, whole genome shotgun sequence.
ACCESSION   DAOQEW010000109 DAOQEW010000000
VERSION     DAOQEW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_12744_cov_8.541099"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..335
                     /locus_tag="PLS35_12185"
     CDS             147..335
                     /locus_tag="PLS35_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19471.1"
                     /translation="MHCLKCKGFMMVERHYTLMNRRLYARCLNCGFWIDLADLLRFFH
                     KVIASSVRGTKVRETYTL"
     gene            382..1311
                     /locus_tag="PLS35_12190"
     CDS             382..1311
                     /locus_tag="PLS35_12190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNI19472.1"
                     /translation="MNRSHRMFPVAVMVIPFLMGALWTGQALAIDNDVDDEVITVPYD
                     PRPVPSAKQAHHSLRWRHVPAHSILLKELKTGTTLYQFESEKRLSPASLTKIMSALVI
                     LEYGHLDDEVTVSPKAARAHKTHLRLRTGQIFRLEDLLKAMLIVSANDACLAASEHVG
                     GDEARFVDLMNAKAAALELHNTHFSNACGFDAPEHYSTAEDLAKLSEIALHHPVFKEL
                     VREEREIIMPINEHRAYVLRTTNRLLGRIPGVQGVKTGFTSKAGRCLIAKVSQDGSDL
                     LLVILNSKRRWNTATSLINYGLRLSDSRAALAR"
     gene            complement(1475..2026)
                     /locus_tag="PLS35_12195"
     CDS             complement(1475..2026)
                     /locus_tag="PLS35_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19473.1"
                     /translation="MGVKEAFMMQRTRTFTALMTMLGSLCLAAVVTAEPYELSKNDVT
                     DPKGVASQDVSLFGIKLGDDEAKALDGLVNEKIPGIKAEQEATFIFLLDQRKPTGPMA
                     GVRVQDGKVDLIFINNRFAFKTRGIFRNVLNSESPDDVRKILGKEDYGDENVMGAMLA
                     YDKQGFQVNYLGKDVNVEFSLPH"
     gene            complement(2191..3129)
                     /locus_tag="PLS35_12200"
     CDS             complement(2191..3129)
                     /locus_tag="PLS35_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNI19474.1"
                     /translation="MELRQLEYFMAVATHQNFSRAAEHVHVSQPSLSIQIGGLEKELG
                     TRLFDRLGRKVVLTQAGELFQVHAERALREVEQAEQVVHELLGAKRGRLIVGTLSTVN
                     SYMIAPLVSRFKLRFPDIHLQVHAQPSSEIVNGLLANRLDIGICLLPLTHAHVTTIPL
                     FEERLALIAPASMNIGKRRARMQDLAQLPLVLMPADYCLRKMVESECAKAGVHPQVVL
                     EMTSPEGILQAVAEGTGATILPELYVKSRLPASQLQIIDLYDPTPRHTVGLAYLTKRH
                     RGQAAEEFAALCETTMRDLQSDSTQPPRNKRKGRAA"
     gene            3310..3786
                     /locus_tag="PLS35_12205"
     CDS             3310..3786
                     /locus_tag="PLS35_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HNI19475.1"
                     /translation="MVSKYGMGAMLLGALALCGGCADTGGGSQTSRAAAAAPAAKSLY
                     DRLGGKPAITAVVDQFVANVAADGRINNRFATTDIPKLKGHLVDQVCQATGGPCRYQG
                     REMKATHLGMQISSADFGALVQDLVAALDTFKVPAGEKGELLALLGSMKKDIVEVP"
     gene            complement(3845..4138)
                     /locus_tag="PLS35_12210"
     CDS             complement(3845..4138)
                     /locus_tag="PLS35_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19476.1"
                     /translation="MSHQSIASLWEEHSRDGWPQFSSPNEGQLMTLDTVIGGCAVFYL
                     DGQPEMDSHRLAILEDCVADLDGLLEDLSDESLGYFQRLRRLATALVDVGRQP"
     gene            complement(4177..4563)
                     /locus_tag="PLS35_12215"
     CDS             complement(4177..4563)
                     /locus_tag="PLS35_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19477.1"
                     /translation="MTRYILAAIAGIWMADGLALLSAPLLVIRRLQESLETAPNLLRW
                     QAIGIGLGLMLLLWSTPLPYQPLWWMTGGAMVIKSCFLTWGPTAWRTPLLSWCFTREA
                     VDYRFFGLWLCTLAVLLLHALGLLHG"
     gene            complement(4596..5852)
                     /locus_tag="PLS35_12220"
     CDS             complement(4596..5852)
                     /locus_tag="PLS35_12220"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNI19478.1"
                     /translation="MTELNTHTFRLAVAQFNEAAEAMQLDTNLRERLKLPQRSLIVSV
                     PVRMDDGRVEVFTGYRVQHDTARGPSKGGIRYHPEVNLGEVAALSMWMTWKCALADLP
                     YGGAKGGVRVDPKQLSRAELQRLTRRYAAEIFPLIGPDKDVPAPDVGTDQQVMAWIMD
                     TYSQQVGYAVQGVVTGKPLSIGGSLGREEATGRGVVYVTLEALQHLKLDVAKATVAIQ
                     GFGNVGSHTARIMQQAGARVIAVSDVSGGLYNPKGLDIPDLLRRYHEHHEPLRDIKLG
                     EPLSNDALLQLDCTVLVPAALSEQITEANAAKLRCRILAEGANGPTTLEADRILTDKG
                     VFIIPDILANSGGVIVSYFEWVQDVQRFFWKAKDIQDRLQDIITSAFHRTLHFSLERR
                     TTMRMAALMSGIDKVAQAHLQRGLYP"
     gene            complement(5852..6685)
                     /gene="lipB"
                     /locus_tag="PLS35_12225"
     CDS             complement(5852..6685)
                     /gene="lipB"
                     /locus_tag="PLS35_12225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNI19479.1"
                     /translation="MSLDRLIHEGAAAPFAGHATAERVEGPRPATLVCGGTMAYDEAW
                     NLQRTCRAERAADRCHDLLLLIEHPSVYTLGRTTQDQHWPGEAQLSRETSIPIIRTER
                     GGSITYHGPGQLVGYPILRLSDYCAGPKAYVRMLEEVLITTLAESGITARRREGLPGV
                     WVGDDRPSKIASIGVRISHGITTHGFALNVCPDLEPFSHIVPCGISDCQVTSMSALMS
                     TTPDLRVVQQQLAALFAARFQLTWSESMTLEQFAAVMEHPNRQLSPSSIHAPANPGGA
                     P"
     gene            complement(6689..7765)
                     /locus_tag="PLS35_12230"
     CDS             complement(6689..7765)
                     /locus_tag="PLS35_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNI19480.1"
                     /translation="MVTGAHSPGAPSRRGLTSRSAWPVLFVFLCNLTLTAFGLVRQDA
                     AAAPLTELPVPAVVSKVRPSVVTVLTRGIPQGGSQHGAPSGSGSGIILDTNGYILTNN
                     HLVQGVTSVVVGLSTGRLTPGRVVARDFLLDLALIRITAPDLVPAEFSQRSTLEIGET
                     VIAIGNPLALKGGSTVTVGVISALDRSVLTPEGETLYDLLQTDAAINPGNSGGPLVDR
                     FGQVVGINVAIAPSAQAISYAIAIEAVTSHIESMMARGSVERPDLGLIPLTITPSVAA
                     SFDLESDRGILALQVDRAKAAGQAGLQSGDVVTAIDNRPLYNMGDFWHAIEHANGQMT
                     FQVATQGKAGTATIIIPRPSLPRP"
     gene            complement(7889..9145)
                     /locus_tag="PLS35_12235"
     CDS             complement(7889..9145)
                     /locus_tag="PLS35_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI19481.1"
                     /translation="MMKHAIAIQDDTDPTVTVEVDPDADDAEASTLLDSIAQGDQPEA
                     DTEEAAPEKSLRSASSPFLLESLYFRSFGERGLLEREEEIELAKKLDLGTRQIRQTLL
                     QAVKACAGLKRAEEAAEHIQTLQTVRRLSGLSATALNQADHALEHLLLDSTAGVKVPA
                     AVRKELEACLATLRSSRVTLETAKDEMVRCNLRLVVNVAKHYTGRGLTLLDLVQEGNI
                     GLMKAAERYQHRKGFKFSTYATWWIRQGITRSLADQSRTIRIPVHQTEASNRILRASR
                     RLVQQLGRQPRLEEVASMMRMRPDRLHETMQAFQEPVALEHQVGDGGTELGELLPDQQ
                     AVPPDAHVNRTELTREMDRILGTLTPREQTVIRLRFGIGEDQARTLEQVGQHLSVTRE
                     RIRQIEAKALKKLKTPMVKEMFAALK"
     gene            9431..10318
                     /locus_tag="PLS35_12240"
     CDS             9431..10318
                     /locus_tag="PLS35_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNI19482.1"
                     /translation="MSQLQNGERVHLVDKKGRQYALTLKAGDIYHFSGETIAHDELIG
                     KPDGSIVTLSKGKRFLALRPTFGEYVLKMPRGAQVLYPKDLSLIPMWADVYPGARVFE
                     AGTGSGALTMALLRAVGPTGLVVTYEARDDFARTALTNIERYMGPVSTLMPMRKNAYE
                     GIDLLEDGRPFDRLVLDLPEPWQVVPHAAKVLRSGGIYLSFVPTIPQVMQTVDALERT
                     SVFGNIETFETLLRTWSIQGRSVRPDHRMVAHSGFLTVARKVEEGWWSRPNRTVDTVD
                     TEDRADRDHQDQEQEDATA"
     gene            10315..11076
                     /locus_tag="PLS35_12245"
     CDS             10315..11076
                     /locus_tag="PLS35_12245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI19483.1"
                     /translation="MNRLQGKVAIITGGNAGIGEAIAKLFADEGAHVVVTGRRKEELD
                     RVVKGIGVNGGRALAVVGSVTDEAHVQDVVAQTTRTFGKVHILVNNAGIGDFGKRLHE
                     TDDATWAKVLDINLTGVFRMTRAAIPELIKAGGGSIVNISTVASLIGIRGLSAYAASK
                     GGLDALTRSVAVDYGQDNIRCNAVNPGLVDTPMAAPLMADPASLEPIMAQYAIRRPGK
                     PEEVAKMVLYLASDEAMWVTGQTFPIDGGMTISKG"
     gene            11143..11997
                     /locus_tag="PLS35_12250"
     CDS             11143..11997
                     /locus_tag="PLS35_12250"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNI19484.1"
                     /translation="MDITPHTQWCGIIGNPVEHSLSPAIHNAAFQKMGLDLVYLAFRV
                     ESLGDALKGIRALGNARGFSVTIPHKVAAIPFLDEVEPTAKHIGAINTIVAEEGKLKG
                     YNTDASGALRALKEGGALLDGHRVLILGSGGAARAIAFALATGTGIAGLTILGIEDAE
                     RQKLVKDLRDKTGVPIEDGPLTLDMLRNWVPHVRTLIHCTPVGMSPKVGESCVPANLF
                     RPELTVMDIVYNPRETKLLQEAKAGGCRTISGLEMFLNQAVLQFELWTKQPAPVDIMR
                     HVLESRFV"
     gene            12064..12582
                     /locus_tag="PLS35_12255"
     CDS             12064..12582
                     /locus_tag="PLS35_12255"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNI19485.1"
                     /translation="MNLVLIGYRGTGKSTVGKVLARKLGRTVVSTDAEIVKRANLPVP
                     EIVKQFGWDHFRDMESTVCRDFAARDQLIIDTGGGAILRPENVAVLQKNGVLVWLTAT
                     VETITRRIGGDTQRPSLTGTKSFTDEIRDVLTERTPKYQAAAAHVISTDGVSTAEVAE
                     RILKLIPHEPGH"
     gene            12667..12743
                     /locus_tag="PLS35_12260"
     tRNA            12667..12743
                     /locus_tag="PLS35_12260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12701..12703,aa:Arg,seq:tcg)
BASE COUNT         2569 a         3616 c         3935 g         2624 t
ORIGIN      
        1 gtaccacgat cttcgggggt gggttgttgg ccggctgcct cgcccttatc ctcaatcgtc
       61 tccgctaaac aaatgacgac gttggtatcg gtctgagatg cgccgagacg tctacgcaat
      121 catgtcgaag gggcaatggt cgggcgatgc actgcctgaa atgcaaagga ttcatgatgg
      181 tggagcggca ttacacgctc atgaatcgcc gactgtatgc acgctgcctg aattgcgggt
      241 tctggatcga tctggccgac ctgctccggt tttttcacaa agtcatcgcc agcagtgtac
      301 gtggcacgaa ggttcgagaa acctataccc tgtgaccgcc agaatccctc tgctcgtcac
      361 aagaaagcat agatattccg catgaaccgt tcacatcgga tgttcccggt ggccgtcatg
      421 gtcattccgt tcctcatggg cgccctttgg actggtcagg ccctggcgat cgacaacgat
      481 gtggatgacg aggtcattac cgttccctac gatcccaggc cggtgccctc ggcgaaacaa
      541 gcgcatcaca gccttcggtg gcggcacgtg ccggcgcata gcattctcct gaaggaattg
      601 aagacgggca cgacgctcta ccagtttgaa agcgaaaagc ggttgtcccc tgcgagcctc
      661 accaagatca tgtctgcgtt agtgatcctg gaatatggcc atctggatga tgaagtgacc
      721 gtcagcccta aggccgcccg cgcccataaa acgcacctgc ggctgcgcac gggacaaata
      781 ttccgactgg aagatttact aaaggcgatg ctgattgttt ccgcgaacga tgcctgcctc
      841 gcggccagcg aacatgtcgg cggcgatgaa gcccggtttg tcgatctcat gaatgccaag
      901 gccgcggcgc tcgaactgca caatacccat ttcagcaatg cctgcggctt tgatgcgccg
      961 gaacattatt cgaccgccga agatctcgcc aagctcagcg agattgcgtt gcaccatccc
     1021 gtgttcaagg aactggtccg cgaagagcgg gaaatcatca tgccgatcaa tgagcatcgc
     1081 gcctatgtct tacgcaccac caatcggctg cttggccgga ttcccggggt gcaaggggta
     1141 aagacggggt ttacctcgaa ggccggtcgg tgcctgatcg cgaaagtctc ccaggatggc
     1201 agtgatctgc tgctggtgat cctcaactcc aagcgtcgct ggaatacggc gaccagcctc
     1261 atcaattacg gcttgcgtct gagcgatagc cgcgccgccc tggcccgata aatttcgcca
     1321 ttgccaatgg ttgcacggaa tccgtgctga tctcgccttc agcgaggctc gcacgagtgc
     1381 gtcctgtgat gcgcagggcg tgatggcgga ctgatcggat agttaaaggc caggaacctg
     1441 cgaggggctg agcgggaatc gcctctgtga cggtttagtg cgggagtgaa aactccacgt
     1501 tgacgtcctt gccgagataa ttcacctgaa agccctgctt gtcataggcg agcatcgcgc
     1561 ccatcacatt ctcgtcgcca taatcttcct tgcccaggat cttgcgcaca tcgtcggggc
     1621 tttcgctgtt gaggacgttg cggaagatgc cgcgtgtctt aaacgcaaaa cgattgttga
     1681 tgaagatgag atcaactttg ccgtcttgca cccgcacgcc ggccattggc ccggtcggtt
     1741 tgcgctgatc gagcaggaaa ataaaggtgg cttcttgttc cgccttgatg ccggggattt
     1801 tttcgttcac cagcccgtcg agagccttcg cttcatcatc ccccagcttg attccgaaca
     1861 gtgacacatc ctggctggcg actcctttcg ggtcagttac gtcgttcttg ctcagttcat
     1921 aaggttcggc agtcacgacg gcagcgaggc acagggaccc cagcatagtc attaaggctg
     1981 tgaatgtccg tgtacgctgc atcatgaatg cctccttaac gcccatgctg aaaatttgtc
     2041 cgggtcgcca ctaccggtca cataaatgag gagagggtca ggcaacaggg cattctatcc
     2101 aagcggctgt ctgactttca aggtcgccgc aggcttattg cgagctgcag acgcgcggat
     2161 tcgggctctg caggaaggcg gtttgagatc ttaggcggct cttcctttgc gcttgttgcg
     2221 gggaggttgt gtggagtctg actgtagatc acgcatcgta gtttcacaca gggctgcgaa
     2281 ttcttcggcc gcttgtcccc gatgccgttt ggtgaggtag gcgagtccga cggtatgacg
     2341 gggcgtggga tcgtacagat cgatgatctg gagttgtgac gccggcagcc gggatttgac
     2401 gtagagctcg ggaaggatcg ttgccccggt tccttccgct accgcctgaa gaatgccttc
     2461 cggggaagtc atttccaaaa ccacctgcgg gtgcacacct gccttcgcac attccgattc
     2521 gaccattttc cgcagacaat aatctgccgg catcaagacc aacggcaatt gtgcgagatc
     2581 ctgcatgcgt gcacggcgtt ttccgatgtt catactggcg ggggcgatga gcgcgagacg
     2641 ttcctcaaac agcggaatgg tggtgacgtg cgcatgagtc agggggagta gacaaatccc
     2701 gatatcaagc cgattcgcca gcaatccgtt gacgatctcg gaagacggtt gggcatgcac
     2761 ctgcagatga atgtcgggaa agcgtagttt gaatcgggag accagcggcg caatcatata
     2821 ggaattgacc gtcgatagcg tgccgacgat caggcgaccg cgtttcgccc cgagcagttc
     2881 gtgcacgacc tgttccgctt gctccacctc ccgcaaggcc cgctctgcat gtacctgaaa
     2941 gagttcacct gcctgcgtca ggaccacctt tctcccgagg cggtcgaaca gacgcgtgcc
     3001 caactccttt tcgagcccgc cgatctgaat cgacaacgaa ggctgcgaga catgcacatg
     3061 ctccgcggcg cggctgaaat tctggtgcgt cgcgacggcc atgaaatatt caagttgccg
     3121 cagttccatg agagttcaga ccagctatag ccaaagacta tggaaagtat gaaaacaata
     3181 tattagacga atagatcata gatctcctaa ggtgaatcgt caaccggaat gtcgcttcac
     3241 aaagcgcttc gcatgtcctg tcggcggcga ggtcaggcaa ccggccattc atcatcaagg
     3301 aggtgggcga tggtatcgaa atacgggatg ggggcaatgc tcttgggggc cctggcgttg
     3361 tgtggcggat gcgccgacac cggggggggc agccagactt cgcgcgcggc ggcggccgct
     3421 cctgcggcaa agtcgttgta cgaccgattg ggcggcaagc cggcgattac ggcggtggtc
     3481 gaccagtttg tggcgaatgt cgcggcggat ggacggatca acaaccggtt tgcgaccacc
     3541 gacatcccta agttgaaggg gcatctcgtt gatcaggttt gccaggcaac aggcggacct
     3601 tgccgatatc aggggcgcga gatgaaggcg acgcacttgg gcatgcaaat cagctcagcg
     3661 gatttcgggg cgttggttca ggatctggtg gcggcgctcg acacgttcaa ggtgcctgct
     3721 ggggaaaagg gcgagcttct ggcattactc ggttcgatga agaaggacat cgtcgaggtg
     3781 ccgtaaatga gcgacggcgg gggtcggcct gagggccggt cctcgcccga gatcgggcac
     3841 agagttaggg ctgccgtccc acgtcgacga gagcggtcgc cagccggcga aggcgctgga
     3901 aatagcccag gctctcatcc gacaagtctt caagaaggcc atcgagatcg gccacacaat
     3961 cctccaggat tgcaaggcga tggctatcca tttccggttg cccgtcgaga taaaacaccg
     4021 cgcatccgcc gatcacggta tccagcgtca tcaactggcc ttcattcggg ctggaaaatt
     4081 gaggccagcc gtcgcggctg tgttcttccc agagagaggc aatggattgg tggctcatag
     4141 gatggtaccg gtccaaggtg aagctgcggc gacgcgttac ccgtgcagca accctaacgc
     4201 atgcaagagc aggacggcaa gcgtgcaaag ccagagaccg aagaatcgat agtccaccgc
     4261 ctcgcgcgtg aagcaccaac tcaacagcgg tgttcgccag gcggtggggc cccaggtcaa
     4321 aaagcagctt ttgatgacca tggcaccacc ggtcatccac cagagtggtt gatagggcag
     4381 cggggtcgac cagagcagga gcatgagccc gagaccgatt ccaatcgctt gccagcggag
     4441 caggttcgga gccgtctcga gggattcctg cagccgccgg atcacgagca gaggggcact
     4501 cagcagggcg agtccgtcgg ccatccagat gccggcaatc gccgcaagga tgtatcgggt
     4561 catactgtga atcgcaggtt cagcatagac agggtttacg ggtagagccc gcgttggaga
     4621 tgggcctggg ctactttgtc gatgcccgac atgagggccg ccatccgcat cgttgttcgc
     4681 cgttcgagcg aaaagtgcag ggttctatgg aaggcgctgg tgatgatgtc ttgtaaccgg
     4741 tcttgaatgt cctttgcttt ccagaagaag cgctgcacgt cctgcaccca ttcaaaatac
     4801 gacacgatca cgccgcccga gttggccaga atatcgggaa tgatgaagac ccccttatcg
     4861 gtgaggatgc gatcggcctc cagtgtcgtg gggccgtttg cgccttctgc cagaatgcgg
     4921 caacgtaact tcgccgcatt ggcttcggtg atttgttcgg acagggcggc aggaaccagc
     4981 acggtacagt ccagttggag caatgcgtca ttgctgagcg gttcgcccaa cttgatgtcg
     5041 cgtagcggct cgtggtgttc gtggtaccga cgcaagaggt ccggaatgtc gaggcccttg
     5101 gggttataga gcccgccgct cacgtcgctc acggcaatca cccgtgcgcc ggcctgttgc
     5161 atgatgcgcg cggtatggga gccgacgttg ccgaacccct ggatggcgac cgtcgctttt
     5221 gcaacgtcga gtttcaggtg ttggagcgct tccagggtca catagaccac accacgaccg
     5281 gtggcttctt ctcgtccgag actgcctccg atggacaacg gctttccggt gacgacgcct
     5341 tgcaccgcat agccgacttg ttggctatag gtgtccatga tccaggccat cacctgctga
     5401 tcggtcccga catcaggcgc aggcacgtcc ttatccggtc cgatgagggg gaaaatctct
     5461 gcggcgtagc gccgggtgag gcgttggagt tccgcacggc tcagttgttt gggatctact
     5521 ctcacgccgc ctttcgcccc gccgtagggc aggtcggcca aggcgcattt ccaggtcatc
     5581 cacatggaaa gggccgcgac ttcgcccaag tttacctccg gatggtaccg gatgccgcct
     5641 ttcgacgggc cgcgagccgt atcgtgctgg acccggtatc ccgtgaagac ttcaacgcgg
     5701 ccatcgtcca tccggaccgg cacgctgaca atcaacgagc gttgcggaag tttcagccgc
     5761 tcgcgcaggt tggtatccag ctgcattgct tcggccgctt cattgaattg ggcgacggcc
     5821 aaacggaagg tgtgggtatt cagttcagtc atcagggcgc tcctccaggg ttggctggcg
     5881 catgaatgga tgaaggggaa agttggcggt tcggatgttc catcacagcc gcgaactgtt
     5941 caagcgtcat ggactccgac cacgtgagct gaaaccgtgc ggcaaagagc gcggcaagct
     6001 gttgctgcac gacacggaga tcgggcgtgg tgctcatgag ggccgacatg gatgtgacct
     6061 ggcaatcgga aatcccgcaa ggcacgatgt gggaaaaagg ctcaaggtca gggcagacat
     6121 tcaaggcgaa gccgtgcgtg gtaatgccgt gggagatacg cacgccgatt gacgcgattt
     6181 tagaggggcg gtcgtcgccg acccagaccc ccggcaggcc ttcacgtcga cgagcggtga
     6241 tacccgactc ggcgagcgtg gtaatcaaca cctcttcaag catgcgaacg taggctttcg
     6301 gccccgcaca gtaatcactc agtcggagga tgggatagcc gaccagctgt cctggcccgt
     6361 ggtacgtaat cgagccgccg cgctctgtcc ggatgatggg gatgctggtc tcccgggaca
     6421 gctgcgcttc tcccggccaa tgctggtcct gcgtggtgcg gcccagggta tacacggaag
     6481 ggtgttcgat caggagcagc aggtcgtgac agcggtctgc cgctcgttcg gcccgacagg
     6541 tgcgttgcag gttccacgct tcgtcatacg ccatcgtccc gccgcatact aacgtcgcgg
     6601 gtcgtggtcc ctcgacgcgt tcggcggtcg catggcctgc gaacggtgcg gcagctcctt
     6661 catggatgag acgatcgagg ctcatggctt acggccgtgg caggctgggg cgtggaatga
     6721 tgatcgtagc cgttccggct ttgccttgtg tcgcgacctg gaacgtcatc tgcccgttgg
     6781 cgtgctcgat ggcatgccag aagtctccca tattgtagag cggacggtta tcaatggccg
     6841 tcaccacatc cccggactgc aatccggctt ggccggctgc tttcgcgcgg tccacctgca
     6901 gggcgaggat gccgcggtcg ctttccagat caaagcttgc ggccacactg ggcgtgatgg
     6961 tcaggggaat tagtccgagg tcaggccgtt caacggatcc acgggccatc attgattcga
     7021 tatgcgaggt cacagcctcg atggcgatag cataactgat ggcttgggcc gacggggcaa
     7081 tagcgacatt gatgccgacg acctggccga agcgatcgac cagcggtcca ccgctattcc
     7141 ccggattgat ggcggcatcc gtttgcagca aatcgtagag ggtctctcct tccggtgtca
     7201 gaacagagcg gtccagcgca ctgatcacac cgacggtcac ggtggagcct cccttcaacg
     7261 ccagcgggtt gccgatggcg atgaccgttt ctccaatctc cagggtggag cgttggctga
     7321 attcggcagg gacgagatcg ggcgcggtga tcctgatgag ggcgaggtcc aggagaaaat
     7381 cacgggcgac gacacgtccg ggggtcagcc ggcccgtaga taatccaacc acgacactgg
     7441 tcacgccttg aacgaggtga ttattggtca ggatatagcc gttggtgtcg agaatgatcc
     7501 cggaaccgga tcccgaaggc gcgccatgtt gcgaaccgcc ctgcgggatg ccgcgtgtca
     7561 gtacggtcac taccgagggc cgtactttcg acacgacggc gggtaccggg agttcggtca
     7621 gcggggcggc agcggcgtcc tggcgcacta gcccgaatgc cgtcagcgtg agattacaca
     7681 gaaagacgaa gagaacgggc caggcagatc gagaagtgag accgcgtctg gatggtgcac
     7741 cgggactgtg ggcgcctgtc accatggaaa gctgatcctc cgccgccgca ttctctcaca
     7801 ctcgcccgtg cggcgaaagc gcgcggaaaa caaaacgggc gaggccgagg cctcgcccgt
     7861 ctcgcgctcc cgtggcgtcg taccgtcgct acttcagcgc ggcaaacatc tccttgacca
     7921 tgggggtctt gagcttcttc aacgctttgg cctcgatctg tcgaatccgt tcacgcgtga
     7981 ccgacagatg ttggcccacc tgctcgagcg tccgtgcttg atcttcgccg atgccgaatc
     8041 gcaaccggat gaccgtttgc tctctgggtg tgagtgtccc gagaatgcgg tccatctcgc
     8101 gggtgagttc cgtgcgattc acgtgggcat cgggcgggac cgcctgctgg tccggcagaa
     8161 gttccccgag ttccgttccg ccatcgccga cctgatgctc cagtgccacc ggttcctgaa
     8221 acgcctgcat cgtttcatgc agacgatcgg gccgcatccg catcatcgat gccacctctt
     8281 ccagccgagg ctgacgaccc aattgctgca cgaggcggcg cgacgcccgg aggatgcgat
     8341 tcgacgcttc tgtctgatgc accgggattc tgatggtgcg cgattgatcg gccagagaac
     8401 gtgtaatgcc ttgccggatc caccaggtgg cataggtgct gaatttgaac cccttacgat
     8461 gctgatagcg ctcggcggcc ttcatcaggc cgatattgcc ttcctgcacc aagtccagca
     8521 aggtgagccc tcgcccggtg taatgctttg cgacattgac gaccagccga aggttgcagc
     8581 gcaccatctc gtctttcgcc gtttccagcg tgacgcgtga actgcgcaag gtggccaggc
     8641 aggcctccag ctcttttcgt accgctgccg gaactttgac tccggctgtg ctgtctagca
     8701 gcaggtgttc cagcgcgtga tctgcttgat tgagcgccgt ggccgataag ccgctgagcc
     8761 gacgtaccgt ctgcaacgtc tgaatgtgtt cggcagcctc ttcagcacgt ttcaatcccg
     8821 cacaggcttt caccgcctgc agcagtgtct ggcgaatctg gcgcgtgccg aggtcgagtt
     8881 tcttggccag ttcaatttcc tcttcccgct ccagcagtcc ccgctcgccg aatgaacgaa
     8941 agtataaaga ttccaaaaga aaaggggagg aagccgaacg caatgatttt tcgggagccg
     9001 cttcctcggt gtccgcctca ggctggtcgc cctgggcgat gctgtccagc agcgtactgg
     9061 cttccgcatc gtcggcgtcc ggatcgactt ccacggtcac cgttggatct gtatcgtctt
     9121 gaatcgcgat cgcgtgtttc atcatgtgtc acccctcttc cccgtcctgg ggatggttgg
     9181 ctacgggaac ccttgggaaa gatgccctca tctctgtaag agaccagaac aggaaaaagg
     9241 attcgtgtcg tctctcattg tccggctgtc cttggtgata ctacgagagc acgaggctgt
     9301 tcagatttct gtctaattga gatagggacg ctcgctgaac tgtcaaggga tcggccggac
     9361 agagtcttaa gcgctgattc gacgggggtt cggttgaaaa cgcgtcggcc atctcctaga
     9421 atgccgacct atgtcacaac tacaaaacgg agaacgtgtt catctggtcg acaaaaaggg
     9481 ccgtcagtac gccctgacct tgaaagccgg ggatatctat cacttcagcg gggagacgat
     9541 tgcgcacgac gagttaatcg ggaagcctga cggatcgatc gtcaccctct cgaaaggcaa
     9601 acgcttcctc gccttgcggc cgaccttcgg cgagtacgtc ctcaaaatgc cccgcggggc
     9661 gcaggtattg tatcccaagg atctatcgtt gattcccatg tgggcggatg tgtatcccgg
     9721 cgcgcgggtg ttcgaagccg gaaccggttc cggcgcgctc acgatggcct tgttgcgcgc
     9781 cgtggggccg accgggctgg tcgtgaccta tgaagcccgt gatgactttg cccgcacggc
     9841 cctcacgaat atcgaacgct acatggggcc ggtgtccacc ctgatgccta tgcgaaagaa
     9901 tgcctatgag ggcattgacc ttctcgaaga cggccgcccg ttcgatcgcc ttgtgttgga
     9961 tttgcccgaa ccctggcagg tcgtcccgca tgcggcgaag gtcttgcgct ccggtggcat
    10021 ttatttgagc ttcgtgccca ccatcccgca agtcatgcaa actgttgacg cgctggaacg
    10081 cacgtcggtg tttggtaaca tcgaaacttt tgagacgttg ctgcgcacct ggtcgatcca
    10141 gggacgtagc gtccgccccg accatcggat ggtggcgcac tcagggtttt tgaccgtggc
    10201 aagaaaggtc gaggagggct ggtggtcccg tccgaacagg accgtcgaca cagtcgacac
    10261 ggaggaccgg gctgacaggg accatcagga ccaggagcag gaggacgcaa cggcatgaat
    10321 cggttacaag ggaaagtggc catcattacc ggtggcaatg caggaatcgg cgaggccatt
    10381 gccaaattgt ttgcggatga aggggcgcac gtcgtcgtca ccggtcgccg gaaagaggaa
    10441 ctcgaccgtg tcgtgaaagg tatcggggtg aacggcggac gagcgctggc cgtcgtcggc
    10501 tccgtcacgg atgaagccca tgtccaggac gtggtcgcgc agaccaccag gacgttcggg
    10561 aaggtgcaca ttctggtaaa caatgccggc atcggcgatt tcggcaagcg acttcatgag
    10621 acggacgacg ccacctgggc gaaagtgttg gacatcaatt tgacgggcgt gttccggatg
    10681 acgcgagcgg cgatcccgga attgatcaag gccggtggcg ggtcgatcgt gaatatctct
    10741 accgtggcca gtctgatcgg cattcgggga ctgtcggcct atgccgcatc caaaggcggc
    10801 ttggatgcat tgaccaggtc ggtggcggtg gattacgggc aggacaatat ccgctgcaat
    10861 gcggtgaacc ccgggttggt cgacacgccg atggcggcgc cgttgatggc ggatcctgcc
    10921 agtttggaac ccatcatggc ccaatatgcc atccgtcggc cggggaagcc cgaggaagtc
    10981 gccaagatgg tgctgtacct cgcttccgat gaagcgatgt gggtgaccgg tcagaccttc
    11041 ccgatcgatg ggggcatgac gatcagcaaa ggctaaggta gcagtctccc gagtcgggca
    11101 aagtcgtggg catcatcgtt gtacgaagca ggatcatcac gtatggacat cacacctcac
    11161 actcaatggt gcggcatcat tggcaacccc gtcgagcatt cgctgtcgcc ggcgatccat
    11221 aatgccgcct ttcagaaaat gggcttggat ctcgtctatc tggccttccg cgtggaatcc
    11281 ctcggtgacg cattgaaagg catacgagcc ttggggaatg cacggggatt cagcgtaacc
    11341 attccgcaca aggtggcggc cattccgttc ctcgatgagg tggagcctac ggccaagcac
    11401 attggcgcca tcaacaccat cgtcgcggaa gagggaaagc tcaaagggta caacacggat
    11461 gcgtcgggag cgttacgggc attgaaagaa ggcggcgcgc tgctcgacgg ccatcgagtg
    11521 ttaatcctgg gttcgggcgg agctgctcgc gccattgcgt ttgcgctggc cacgggaaca
    11581 ggcatcgcgg ggctgactat tctcgggatc gaagacgcgg agcggcagaa gctggtgaag
    11641 gatttgcgcg ataagaccgg agtccccatc gaggacggtc cgctgacatt ggacatgctt
    11701 cggaattggg tcccacatgt ccgcacgttg atccactgca cgccggtcgg gatgtccccc
    11761 aaggtcgggg agtcgtgcgt gccggccaat ctgttcagac ctgagctcac ggtgatggat
    11821 atcgtctaca acccgcgcga aaccaagttg cttcaggaag cgaaggccgg cggatgccgg
    11881 accatttccg ggttggagat gtttctcaat caagccgtgc tgcaattcga gttgtggacg
    11941 aaacagccgg cgcctgtcga tattatgcgg catgtattgg agtcgcggtt tgtctagcga
    12001 atcagcccct ctccgcgcca aatccgcagt ggcgaccgca tcctttccag cttggaaact
    12061 cctatgaatc tcgtcctgat cggatatcgc ggaaccggta agagtacggt gggcaaagtt
    12121 ctcgcccgca agctcggccg cacggtcgtc tcgacagatg ccgagatcgt caagcgcgca
    12181 aacctccctg tgccggaaat cgtcaagcaa ttcgggtggg accattttcg tgatatggag
    12241 tcgaccgtgt gtcgggattt tgccgcacgg gatcagttga tcatcgatac cggtggcggt
    12301 gccattcttc ggccggagaa tgtggcagtg ctgcaaaaga acggcgtgtt ggtctggctt
    12361 actgcgaccg tcgaaacgat tacacgccgg atcggtggcg atacgcaacg gccgtcattg
    12421 acaggcacaa aatccttcac cgacgaaatc cgtgacgtcc tgaccgagcg gaccccgaag
    12481 tatcaggctg ccgccgccca tgtgatctcg actgacggcg tctccacggc agaagtggcc
    12541 gaacgcattc tcaaactgat cccgcacgag ccaggccatt gagtcggaga ccgctgcgac
    12601 tgaaatcagc cgtgcctcgt caccttcctt gacttcccct cagtaacatg gttttaatcg
    12661 ccgcgcgcgc ccgtagctca attggataga gcatcggact tcggatccga gggttggggg
    12721 ttcaagtccc tccgggcgca ccaa
//
LOCUS       DAOQEW010000110        12411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1474_length_12411_cov_8.439624, whole genome shotgun sequence.
ACCESSION   DAOQEW010000110 DAOQEW010000000
VERSION     DAOQEW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_12411_cov_8.439624"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /gene="malQ"
                     /locus_tag="PLS35_12265"
     CDS             <1..1651
                     /gene="malQ"
                     /locus_tag="PLS35_12265"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNI19486.1"
                     /translation="NWGVGDFTDLARLVEWAGKELGAGIIGLNPLHALKNSRPHHLSP
                     YSPTSRLFLNELYIDLDRLPEYHASPDAQRLRQSPEFQSGVEQARAADFVDSESVVKA
                     KRTMLDLAYRQFLTENYAGTEPSLQPTSARGWLLERFIRDEGESLERFALFQVLEEDR
                     RLIEQAPKLWPEWPEQYRNPGTPALREFARRHRKRVRFFQYVQWVAADQLRAANTRAS
                     QVHMTVGLYPDLALGSDRNGAEAWMLQDTLALGADCGAPPDAFAPQGQNWGFAPFNPL
                     RLKATGYRSFIELLRKTLRQGGAIRIDHVMMLFRLFWVPRGLTAAAGAYVQYPAEDLL
                     RILALESMRAQTLVIGEDLGTVPDYVREQLARYKILSYRVFYFERNWDGSCKPPSAYP
                     DQSLAVVTTHDLPTLTGYWSGEDIRLRARLGMYVHEQAVQQAFEERARDKNQFLAALQ
                     SAGLLPPGVSAQQESVPAMTPDLCRAIHLYLAGSPAWVVLANLDDVIGEVTQMNLPGT
                     VDAYPNWSRKLSLSLEELQQDERVQALAAAIRTLRPSTTPA"
     gene            1816..2967
                     /locus_tag="PLS35_12270"
     CDS             1816..2967
                     /locus_tag="PLS35_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19487.1"
                     /translation="MKNRNQLVLGIAAVLFLIVTVMEWMFPLNVVGAFGFVLPILLVA
                     TVRNRQLMVMTLALCILVTFIGLFQPAKKKERFTTVVFNRIMVVFVLAGVAYIGVTWE
                     ERKAREEAARAALATQTEHLLRANTQLGELKDTLARSERLAAVGQLVASVAHEVGTPL
                     HSIAWHVEALAEEPGLTAEMQKRIGIIDEQLNRVVRIIQDLLSSTRQRKPEPTWYPAE
                     RLIEPVVALMEPGYHAKGVALQAEVTPAVSVWADAEKIHQVLVNLLTNALAATSTGGQ
                     VVVSVTARPASAEERERGSCTGRTDLDTTVLLVVSDSGCGMPREDMDRAFQPFFTTKA
                     IGKGTGLGLFLSREVVVAHGGQLTLESEVGKGTTATVILPGLPTHPGAA"
     gene            2981..4375
                     /locus_tag="PLS35_12275"
     CDS             2981..4375
                     /locus_tag="PLS35_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI19488.1"
                     /translation="MNQAKLFVVDDDETARTLLAEALAKEGYAVEAFASGQAAVERGK
                     QAPPDVVLTDIRMEQGDGFLVLKEFKRFSPDTSIVLLTAFGSLEGAIEAIKQGAYDYL
                     AKPFKRDEIRLVVQRSLEHCRLVRENARFREDARVREPWSHLVGSSPAMLEVYKLVAR
                     VSDGRSTVLIEGESGTGKELIARAVHLNSPRREKPFIPVNCGALPDHLLESEMFGYEK
                     GAFTGAVGAKAGLFEAANGGTLFLDEIGDLGQALQVKLLRVMQEQEVRRVGSTSSVKV
                     DVRIIAATNRDLAARVKEGKFRDDLYYRLNVVRIALPSLAQRQEDIPMLAHHFLQKYA
                     KQSSHVRGFAPDTMALLKRYQWPGNVRELENAVERAVSLSHGPLLLPEDLPESIRAEP
                     GPSDQPAGGLSEGDPDALLTLDEVEKRHLSRVLKETRGNKVKAAKILGIDRRTLYRMA
                     ERFGLDFGDESDEK"
     gene            complement(4382..4810)
                     /locus_tag="PLS35_12280"
     CDS             complement(4382..4810)
                     /locus_tag="PLS35_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19489.1"
                     /translation="MIAPLLFAIVVAGCAGAPAAPELDVMEVTLEAPADKVQTAVADV
                     LTQGGYSVDQDESGNVTTGMRQEVRGPWNNLLRWRFGVGKSRVEARIVRQDVSTTRLR
                     LQVFYRGKDGIFDTWQDAETPLPQSAANEIRLIKNALRLL"
     gene            complement(4816..5070)
                     /locus_tag="PLS35_12285"
     CDS             complement(4816..5070)
                     /locus_tag="PLS35_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNI19490.1"
                     /translation="MPDRAYVLINVHPGQTADVVKALADIKEIKQIDPCWGKPDIFTI
                     VEIPHQDALTQLVLAKIHAIPGVEQTDTHNVYRLQETKPK"
     gene            complement(5191..6909)
                     /locus_tag="PLS35_12290"
     CDS             complement(5191..6909)
                     /locus_tag="PLS35_12290"
                     /inference="COORDINATES: protein motif:HMM:NF037406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19491.1"
                     /translation="MARAKATEALRSKTKPNAVDELSRGIATLRELSVQIDDLSRDGF
                     PYREAVRARTELSFRETIRRLFGEKSPEYQTHKNHKLKVGTRAESTQSTALLKEFIAA
                     LEIQKADLLGLKPEEVIAMVATEPGRPPLTMVPPVTSTDANTMSSATPPALPAAVAAP
                     IPTTTATIASAVSNPPLSPQAPPSETSRIVAPSTSPSASDTLPPPVRDTLAPRPVQPS
                     ASVSSASGESTQVSQLSSTPIPTTPSPMPPPIVPAPSPGTSGSSAKAAGDATIPTTTI
                     PPITSARSTVPQGTLPPVPHTTLRPRPAPLVSTDIPASTQLSTNVPPPLTPTPAPASE
                     PPRQIVPSVGATTADSSPIPVQENRPTPPLVQTMASTSSTASPAPQQEPPAPSIVAQA
                     SAPVRSSAPASTQIPIPEKVPEAKTSAEHGTLEILRRVCARFHLVARQLRLRKEYRPT
                     LEITDEYDLQDLFYALLRLQFDEVGTEEWTPAYTNGAPRTSYLLDWEKTVVVVKQTRS
                     GLTTKDLAEQIAADTARYSARPNGANFVCFVYDPDGRVGNPRGLEADLSSTEGTYRVE
                     VIVAPK"
     gene            complement(7019..7513)
                     /locus_tag="PLS35_12295"
     CDS             complement(7019..7513)
                     /locus_tag="PLS35_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19492.1"
                     /translation="MTTDKIAHATILLVDDDPSTLLLASKPLQDQGYTVLQAPGSAEA
                     LRLYAEHPRPIHLVVTDIFLPPPGFQLSVEENPYPRVNGLEMVERLLEKKRDIRIILM
                     SSTPGPDLQGRGLIREGLPFLKKPFTSDALLTLIRDTLAGPPATQGPTKPSASGKGEV
                     EWVD"
     gene            complement(7551..8402)
                     /locus_tag="PLS35_12300"
     CDS             complement(7551..8402)
                     /locus_tag="PLS35_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19493.1"
                     /translation="MVRYPLIFLLSWSFFAAPIAEANENHPFWTEQALFHFGEDVFFT
                     GRASCAPNVEEGRQRAYLAAVQEVKNFTRAQEIGGFPLDTQMIFEDSRPADCAPGLVT
                     VWRLLRAPRLALDGLARRGTPGKSADISPILAQVAAVRNLTPRVGMLRDEVWNRYGLP
                     RSIWARPEKGELIWDYSQFGLTIVFNQEDVLTRWRLTGPNPRSSEDTSPAPSQPEPLS
                     GKDLPAIDLTARLQQLEEQQDLERRHEAQRYCSLRFAEVPDTLRPTQGTCEQREYQRL
                     KERHPRF"
     gene            complement(8552..9235)
                     /locus_tag="PLS35_12305"
     CDS             complement(8552..9235)
                     /locus_tag="PLS35_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19494.1"
                     /translation="MKSANALIGLVLLLLATTGCHGYTLLETPTTDGFHSKLPAPYTR
                     AVVWGGRPDTVQSASTWLLKKGLLVVDQTKVLQAAADQKVSLSGYQYLETDVLRMAKL
                     VGARLVVFSNAEVGSWEALDWSNGVPRSQRVYSATIALRAVDVNTGEIEWSGKAQSTE
                     RFSNIEEGISFLTCHALATAWGMRNPGSLAPENICPPGSGLMTTEGTKHHATPAASTR
                     ENLTPSMNN"
     gene            complement(9564..9695)
                     /locus_tag="PLS35_12310"
     CDS             complement(9564..9695)
                     /locus_tag="PLS35_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19495.1"
                     /translation="MIEFPLILIGYALLPIIAIQLMIMADRPQQKGEQNAFPQVEIL"
     gene            complement(10029..10916)
                     /locus_tag="PLS35_12315"
     CDS             complement(10029..10916)
                     /locus_tag="PLS35_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNI19496.1"
                     /translation="MVKVVRTEYPQRSGLIAINLAAIIFGTAALYGRLDIAPLWIVTM
                     RGAFASVALAGVGLLSGGLGLRPTIAQLKRLLVTGILLAIHWVTFFQSVQLAGVAIAT
                     LTFAVFPLFTVLLETMKARRRPSLLELSAGVAIVAAVALLVDTKDSDATLTGTIVGLG
                     SALAFAVFGIKAQILPTEITPLMVSLIQNLVVSLSLTPLLAVSSPAPKTTTDWLGLLL
                     LGVVTTAFMHQLYLFALKRLSATTCSGFIALEPVYAIVFAAVFFGEPLTLWVVVSAVL
                     IIGASVVLLRAERVTVVLD"
     gene            11762..12256
                     /locus_tag="PLS35_12320"
     CDS             11762..12256
                     /locus_tag="PLS35_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19497.1"
                     /translation="MLARMLRSLALVTCIAGGAGGCLLSQSGSSTTLPSSAFFSIEPD
                     QLGALQEIGHAQDLLMKQCHKGATCEEAYYIRGLVALFENRANAISVFQELHTVMPNS
                     RYDSATTGWLNLLQDRASSSSESKAVLGQLKQQVLLKLLEHRGGIVAQGTEAQNRRIA
                     ELNE"
BASE COUNT         2513 a         3230 c         3971 g         2697 t
ORIGIN      
        1 gaattggggc gtcggcgatt ttacggattt ggctcgtctg gtcgaatggg cgggcaagga
       61 attgggggcg gggatcatcg gattgaatcc cttacacgcg ctcaagaatt cgcgccctca
      121 ccacctcagt ccctactctc cgacaagccg cctctttttg aacgaactct atatcgatct
      181 ggatcgcctg cctgaatacc atgcgtcgcc cgacgcccag aggctgagac agagccctga
      241 atttcaaagc ggcgtggagc aggcacgcgc ggcagacttc gtggactctg aatccgtggt
      301 caaggccaag cgcacgatgc tggatttggc ctatcggcaa ttcctgacgg agaattatgc
      361 gggaacagag ccgtcgctgc agcccaccag tgccagggga tggttgttgg aacgattcat
      421 ccgggatgaa ggcgagtcgc tggaacgatt tgccttgttc caggtgctgg aagaagaccg
      481 ccgtctgatc gaacaggctc cgaagctctg gcctgaatgg ccggaacagt accggaatcc
      541 cggcacgccg gcgttgcggg aatttgcgcg gcgccatcgc aaacgcgtta ggttttttca
      601 gtatgtgcag tgggtcgcgg ctgatcaact tcgcgccgcc aatacccgcg cctcacaggt
      661 gcacatgacg gtcggcctgt atcctgatct ggcgttggga agcgatcgca acggcgcaga
      721 agcctggatg ttgcaggata cgctggcgct gggtgcagac tgcggcgctc caccggatgc
      781 gttcgctcca caggggcaga attggggatt cgcgccgttc aacccgctgc gtctgaaggc
      841 gacagggtat cgttcgttca tcgagctcct ccggaagacc ttgcgccagg gcggagcgat
      901 tcgcatcgat catgtcatga tgttgttccg gctgttctgg gtgcctcgcg ggttgactgc
      961 cgcagccgga gcctatgtgc agtatccggc cgaagatctg ctgcggattc ttgcgctgga
     1021 gagcatgcgt gcgcagaccc tggttattgg tgaagaccta ggtacggtgc cggattatgt
     1081 tcgcgaacaa ctcgcccggt acaaaatcct gtcctaccgc gtgttctact tcgagcggaa
     1141 ctgggacggg tcctgcaagc cgccgtcggc ttatcccgat caatcgctcg ccgtcgtgac
     1201 gacgcatgat ctcccgacat tgaccgggta ttggagcggg gaagatatcc gactgcgcgc
     1261 caggctcggg atgtatgtgc atgaacaggc ggtacagcag gccttcgagg aacgggccag
     1321 ggacaagaat cagtttctcg cagcgctgca gagcgccggg ctgttgcctc ccggtgtaag
     1381 cgcgcagcag gaatctgtgc cggccatgac accggacttg tgccgggcga ttcacctgta
     1441 tcttgccggc tcgccagcct gggtggtatt ggccaacctt gatgatgtga tcggtgaagt
     1501 gacacagatg aatttgcctg gtaccgtaga cgcctatccg aactggtcac gcaaattgtc
     1561 gctgtcgctg gaggaactcc aacaggatga gcgtgtacaa gctctcgccg ccgcgattcg
     1621 tacgctccgc ccttctacca cgcccgctta ggccaatgcg ggacacagct tcggcgtggt
     1681 gcctgccccg ccagcggttt caacagtcta gacaagcgcg gtggggcgag ggctcgaggt
     1741 gcctagcatc tgggtggccc tggtgacgcg aacattactg gcacgcccgt tctttcccca
     1801 aggcgtccga tcgtgatgaa gaaccgcaat cagctcgtcc tcggtattgc cgccgttctg
     1861 ttcctcatcg tgacggtgat ggaatggatg tttccgctga atgtggtcgg cgcattcggg
     1921 tttgtgttgc cgatattgct cgtggccacc gttcggaatc ggcaactcat ggtgatgacg
     1981 ctcgcgctct gtatcctggt gacattcatc gggctgtttc agccggcgaa gaaaaaagaa
     2041 cggttcacga cggtggtatt caatcgcatt atggtcgtct tcgttctggc tggggtggcc
     2101 tatatcggcg tgacctggga ggaacggaag gcccgggaag aagcggcccg ggctgcattg
     2161 gcgactcaaa cggagcatct gctccgcgcc aacacgcagt tgggcgaact taaggataca
     2221 ctggcgcggt ctgagcggtt ggcggcggtg gggcaattgg tcgcatcggt ggcgcacgag
     2281 gtggggacgc ccttgcactc catcgcctgg catgtggagg cgttggcgga agagcctggg
     2341 ctcacggcgg agatgcaaaa gcggatcgga atcatcgatg aacagttgaa tcgtgtggta
     2401 cggatcattc aagatcttct ctcgtcgacg agacaacgta agcccgaacc gacctggtat
     2461 ccggccgagc ggctcattga gccggtggtg gcattaatgg agccggggta tcacgcgaag
     2521 ggcgtggcgc ttcaggcgga ggtcacgccg gcggtgtcgg tgtgggcgga tgccgaaaag
     2581 attcatcaag tcttggtgaa tcttcttacc aacgcgctgg ctgcgaccag cacgggaggg
     2641 caggtggtcg tgtcggtgac agcacgcccg gcatcagcgg aggagcggga acgcggatcc
     2701 tgcacgggcc ggactgatct cgacacgacg gtcctgctcg tggtgtccga ctccggatgc
     2761 ggaatgccgc gtgaagatat ggatcgggcc tttcagccgt tttttaccac gaaggcgatc
     2821 gggaaaggga ccggtttagg attgtttctc agccgggaag tcgttgtggc acacggcggt
     2881 cagctgacac tggagagtga agtcggaaaa gggacgacgg cgaccgtgat ccttcccggg
     2941 ctgccgacgc atcccggcgc agcgtaggag cgcaaggctg atgaatcagg caaaactgtt
     3001 tgtggtcgat gatgatgaga ctgcccggac cttactggcg gaagcgctcg cgaaagaagg
     3061 gtatgcggtc gaggcctttg cgagcgggca ggcggcggtg gagcgtggca agcaggcgcc
     3121 tcccgacgtg gtgctcaccg acatccgcat ggagcaaggc gacgggtttt tggtgttgaa
     3181 agaattcaag cgattcagcc cggatacctc gatcgtgttg ctgacggcgt tcggatcctt
     3241 ggagggcgcg attgaagcca tcaagcaagg tgcctatgac tacctcgcga aacccttcaa
     3301 gcgggacgag attcgcctgg tcgtgcagcg cagcttggag cattgccgtc tcgttcggga
     3361 aaatgcccgg tttcgtgaag atgcccgcgt gcgtgaaccc tggtcgcatc tggttggcag
     3421 cagtccggcg atgttggagg tctacaagct cgtcgcccgc gtatccgatg ggcggagcac
     3481 ggtgctgatc gaaggggaaa gcggaaccgg caaggagttg attgcccgcg cggttcattt
     3541 aaacagtccg cgtcgcgaga aaccatttat tccggtgaac tgcggtgctc ttcccgatca
     3601 tctgctcgaa tcggaaatgt tcggttatga aaaaggcgcg tttaccggag ccgtgggcgc
     3661 gaaggctgga ttatttgagg cggccaatgg cgggacgctg tttttggacg agatcgggga
     3721 tctcgggcaa gcgcttcagg tgaagctgtt gcgtgtgatg caggaacagg aggtgcggcg
     3781 ggtgggcagc acgtcctctg tgaaagttga tgttcgtata atcgccgcca cgaatcggga
     3841 ccttgccgcc cgggtcaaag agggcaaatt ccgcgatgat ttgtactatc ggttgaatgt
     3901 ggtgcggatt gcactgccct cgttagctca gcgacaggaa gatattccga tgctcgccca
     3961 tcatttttta cagaagtatg ccaagcaatc ttcccatgtg agagggttcg cccctgacac
     4021 gatggcgttg ttgaagcgct atcagtggcc gggcaatgtg cgagagctgg aaaatgccgt
     4081 ggaacgggcc gtgtccttga gtcatggtcc gctgctgtta cccgaagatc tccccgaatc
     4141 gatccgagcc gagcccggtc cgtccgacca gcctgccggt ggactgtccg agggggatcc
     4201 tgacgcgttg ctgaccttgg atgaagtaga gaagcgtcat ctctcacggg tgctgaaaga
     4261 gaccaggggc aataaggtca aggcggcgaa gatcctggga atcgaccggc ggacgttgta
     4321 cagaatggcg gaacgattcg ggttggattt cggcgatgag agtgacgaga agtagtctgg
     4381 actacaacaa ccgcagggcg ttcttgatca agcggatctc gttggcggcg ctctgcggca
     4441 agggggtctc cgcatcttgc caagtatcga agatcccgtc tttgccccga tagaacacct
     4501 gcagccgtag ccgtgtggtg ctgacatctt gccgcacgat gcgcgcttcc acccggcttt
     4561 ttcctacacc aaatctccag cgcaacaagt tgttccacgg acctctgact tcctgtctca
     4621 ttccagtcgt cacgttgccg ctctcatcct gatcgacgga gtagcctccc tgtgtgagca
     4681 catcagcgac agcggtttgg accttgtctg caggagcttc cagcgtcact tccatcacat
     4741 ccagctcagg agcggcgggg gctccggcac atccggctac cacgatggcg aacagaagcg
     4801 gcgcgatcat gcggatcatt tgggttttgt ctcctgaagc cgatagacgt tgtgggtatc
     4861 ggtttgctcg acgccaggaa tggcgtgaat cttcgccagc acaagctggg ttaaggcatc
     4921 ctgatgagga atttccacga tggtgaagat gtcgggctta ccccaacagg gatcgatttg
     4981 tttgatttct ttaatgtccg ctaaggcctt cacgacatca gcggtttgac cgggatggac
     5041 gttgatcaat acgtaggcgc ggtcgggcat ggagaggatc tcccttgaac tagcgcaggt
     5101 ggttaggcct cacgcgaatc gaaacctatc ggattgctcc ggatgaagtc aacacgatgc
     5161 acgcacgtct gccttggtga cgatgcgatg tcacttgggc gccacaatga cttcgacgcg
     5221 gtatgtcccc tcagtggatg agaggtcagc ctccaatcca cgcggatttc ccacacgtcc
     5281 gtccgggtca tacacaaagc agacgaagtt cgctccattc ggtcgcgcgg agtagcgggc
     5341 tgtgtctgcg gcgatctgtt cggcgagatc tttcgtcgtc agtccggagc gggtctgttt
     5401 gacgaccacg accgtctttt cccaatcaag gagatagctg gtccgcggcg cgccgttcgt
     5461 ataggccgga gtccactctt ctgtgccgac ctcatcgaac tgcaatcgga ggagcgcgta
     5521 gaagagatcc tgcaagtcat actcgtcggt gatttcaagc gtcggacggt attcttttct
     5581 gagtcgcagt tgtcgggcga cgaggtgaaa ccgggcgcag actcttctca gtatttccag
     5641 tgtgccatgc tcggccgagg ttttcgcctc gggaaccttc tccggaatgg ggatctgggt
     5701 ggatgcgggc gctgatgatc ggaccggtgc ggatgcctgc gccacgatgg atggagcggg
     5761 cggttcctgc tgcggtgcag ggcttgcggt actcgatgtc gacgccatcg tctgaacgag
     5821 cggaggcgtg ggtcggttct cttgaacagg aatagggctt gagtctgccg tggttgcacc
     5881 taccgaggga actatctgcc ggggtggctc agaagccgga gcaggcgtcg gcgtcagggg
     5941 gggcggaaca ttcgttgaca gctgagttga agcgggaatg tcggtactga cgagtggtgc
     6001 tggtcgcggt cgcaaggtcg tgtggggaac cggtggaagg gtgccttgag gcacggtact
     6061 tcttgccgat gtgattggag gaatggtggt tgtaggaatc gttgcatctc ccgcggcctt
     6121 cgcactgctc ccgctggttc caggcgatgg tgccgggaca attggagggg gcatgggcga
     6181 tggcgtcgtc ggaatcgggg tactactgag ctgtgatacc tgcgtggatt cgccagaagc
     6241 ggaactgacc gaagccgatg gctgaacggg gcgaggtgct agggtgtccc gcacaggcgg
     6301 gggcagggtg tccgaggccg atggcgacgt tgaaggggcg acgatccgtg atgtttctga
     6361 gggcggagct tggggcgaaa gaggcggatt ggagacggca ctggcaatgg tcgccgttgt
     6421 agtggggatc ggagcagcaa cagctgccgg cagagccgga ggtgtcgcac tcgacatggt
     6481 gttggcgtcg gtcgatgtga cgggcggcac catcgtcagc ggcggacgac ccggctccgt
     6541 cgcgaccatg gcgatgacct cctcaggctt gagaccgagc aagtcagctt tttggatttc
     6601 gagtgcggcg atgaactcct tcagaagcgc cgtgctctgt gtggattctg cgcgagtgcc
     6661 gaccttgagt ttatgatttt tgtgcgtctg atattccgga gatttttcac caaacaatcg
     6721 gcggatggtt tcccgaaagg agagttcggt tctggcgcgg actgcttccc ggtagggaaa
     6781 tccgtctcgg ctcagatcgt caatctggac gctcagctcc cgcagggtgg cgatgcctcg
     6841 tgagagttca tcaaccgcgt tcggtttggt tttgctacgt aatgcctcgg ttgcttttgc
     6901 tcgtgccatg cgagtctccc tctcgtgaga aaagtctatc cgagggggtg gagcctgaca
     6961 agcaatccct gcttttactc gtggtcgacg tggaggtgcc ggcgaggatg gatccgggtc
     7021 agtcgaccca ctcgacctct cccttgccgg atgcagaagg ttttgtcggg ccctgtgtcg
     7081 ccggaggtcc ggccaaggtg tcacggatga gtgtcagcag cgcgtcgctg gtaaacggtt
     7141 ttttgaggaa gggcaagccc tcacggataa ggccgcggcc ctgcaggtca ggtcctggag
     7201 tgctggacat cagaatgatg cggatgtctc gctttttctc gagcaggcgt tcgaccatct
     7261 cgagaccatt cacgcggggg taaggattct cctcgacgga aagttggaag ccggggggtg
     7321 ggagaaagat gtccgtgacg acgagatgga tgggccttgg gtgttccgca tagagcctga
     7381 gcgcttcggc actgcccggt gcctgcagta ccgtgtatcc ctgatcctgc agtggtttgg
     7441 acgccagcag gagcgttgag gggtcgtcgt caacgaggag gattgtcgcg tgcgctattt
     7501 tgtcggtggt catgccggcc ctccttcgtg gcagtgactc gggagacgga tcaaaatcgg
     7561 ggatggcgct ccttgagtcg ttggtattcc cgttgctcac aggtgccttg ggtgggacgc
     7621 aacgtgtctg gcacctcggc gaaacgcaac gagcaatagc gctgggcttc atggcggcgt
     7681 tcgaggtcct gctgttcttc aagttgctgg aggcgggctg tgaggtcaat agcaggaaga
     7741 tccttgccgc tgaggggctc cggttgggat ggagccgggg aggtgtcttc gctggatcgt
     7801 ggattgggtc cggtcagccg ccagcgcgtc aggacgtctt cctggttgaa gacgatggtc
     7861 aggccgaact gcgagtaatc ccagatcagt tcaccctttt cggggcgcgc ccagattgat
     7921 cgcggcaggc cgtatcgatt ccagacctcg tcacgcaaca ttcctacacg aggcgtgaga
     7981 tttctcactg ctgcgacttg tgcgaggatc ggcgaaatgt cggccgactt cccgggggtg
     8041 ccgcgtcttg cgagtccgtc cagggccagg cggggcgcac gtaaaaggcg ccagaccgtc
     8101 accaatcccg gggcgcagtc agccggacgg ctgtcttcaa aaatcatctg ggtgtccagc
     8161 ggaaatccgc cgatttcctg ggcccgcgtg aaatttttga cctcctggac agcggcgagg
     8221 taggcgcgtt gccgcccttc ttctacgttg ggtgcgcagg aggctcgccc cgtaaaaaag
     8281 acatcttcac cgaaatgaaa gagggcttgt tccgtccaga acgggtgatt ctcgttggcc
     8341 tcggcgattg gcgccgcaaa aaagctccaa ctcaggagaa aaatgagcgg gtaccgcacc
     8401 ataggcaaca ctcaataaaa gagccgtaag agggaggcac tcatgcagta taacgcgccg
     8461 gacaagaaaa tcgacaaaag agcgagtttg tgcgagaggc actgtaggaa agagcgtcgg
     8521 cgaggcaata gcccgcgaag agatgcgcgg cctagttatt catggagggg gtaagatttt
     8581 ctctcgtcga ggctgcaggg gtggcgtggt gcttggtgcc ctcggtcgtc atgaggccgg
     8641 accccggagg acagatgttt tcgggagcga gggagccggg gttacgcatg ccccaggctg
     8701 tggctaaggc atggcaggtg aggaagctga ttccctcctc gatgttgctg aagcgttcgg
     8761 tggactgagc tttcccgctc cattcaattt cgccggtgtt cacatcaacg gcccgcagcg
     8821 caatcgtcgc agagtacacg cgttgactgc gcggtacccc gttactccag tccagtgctt
     8881 cccaagaccc tacctcggca ttgctaaaga ccaccagtcg ggcaccaacc agtttcgcca
     8941 ttcgaagcac gtctgtttcg agatactgat atcccgaaag actgactttt tgatcggctg
     9001 cggcttgcaa gaccttggtt tggtctacga cgagaagacc ttttttaagc agccaggtac
     9061 ttgcgctttg caccgtatct gggcgtccgc cccaaacgac cgctctggtg tagggcgcag
     9121 gaagtttaga gtgaaatcca tcagtcgtgg gagtttctaa gagggtatac ccgtggcatc
     9181 cggtcgtggc gaggaggagc agaaccaaac cgatcaatgc attcgccgat ttcatggact
     9241 ctttatggaa ggatgtgtgc tgactgctcg ccatgaatct ttatcaagaa cggttcgtgc
     9301 ttcctggatg ggcactcaat gtttcggcct cattatacag gattgattct acctacgtct
     9361 gctgcattcg tcgagtacat tgggtgttgg cgctctcgag ataccgctac ttacttacag
     9421 gatgaaattg tgggagcctc ggccggcggt ggtgctcaac gcaagtctgg gatgtgaatt
     9481 gccgcagtcg ggaaggaggc gggagtcaac gacggcctgg aaaggcggcg gcttcactca
     9541 tggcttgtga agccgccgca ggcttacagg atttcgacct gtggaaaggc gttttgttcg
     9601 cccttctgtt ggggacggtc agccatgatc atcaactgaa tggcaatgat cggcagaagt
     9661 gcatagccta taaggatcag cggaaactca atcatggtgg aactcctttt gttatgtaga
     9721 gcctccactg caagctcgat accgttctcc cacggagtgc caagctattg attgtactga
     9781 tgctcgagat ggtttatgcc ggcagtgctc ctttggaggc cgaaactcaa gggagagaac
     9841 tgtgcaatga gaacaatttg cgctgagccg gaagacctaa gggtaacggg acgaaagacg
     9901 gcctgagtcc ttgcgatcca agagatgggg gctcgcgttg cagaggcgta ggagaagggg
     9961 ggagaagatg gcagatcttg ggctgctgtt ctgaggggct ggtggcgctt cggacctgtg
    10021 atgaatagtc agtccagaac gaccgtgact cgctcggctc gtaacaacac gacagatgca
    10081 ccaataataa gcaccgcgct caccacgacc cagagcgtca agggttcccc aaaaaatact
    10141 gcagcaaaga cgatcgcgta caccggctca agggcaataa acccgctgca cgtcgtggcg
    10201 gacaatctct tcaacgcaaa caggtagagc tgatgcatga aggctgtcgt cacaacgccg
    10261 agcagcagga ggccaagcca gtcagtggtg gttttcggcg ctggcgagct gaccgcgaga
    10321 agcggcgtga gacttaagga caccacgaga ttttgaatga gtgacaccat gaggggtgtt
    10381 atctcggtgg ggaggatctg tgccttgatc ccaaacaccg caaacgccaa tgcagaaccg
    10441 agcccaacga tcgtgccggt gagtgtggca tcggaatcct tcgtatccac taacaacgca
    10501 actgccgcaa caatcgcaac cccggcgcta agttctagca ggcttgggcg ccggcgcgcc
    10561 ttcatcgttt caagcagcac cgtaaacaga ggaaagactg cgaacgtcag tgtcgcgatg
    10621 gccacaccgg caagctggac tgattggaag aaggtgaccc aatgaatcgc cagcagtatg
    10681 cctgtcacga gcaggcgctt gagttgtgcg atggttggac gcagacccag gccgccactt
    10741 agtaagccca ctccggctag tgccactgaa gcaaacgctc ctctcatcgt cacaatccac
    10801 aatggtgcga tatctaatct gccgtacagt gcggccgtgc caaagatgat ggcggcaaga
    10861 ttgattgcga tcagtcctga tctctgaggg tattctgtcc tgacgacctt gaccatattt
    10921 cttaatgacc gtgggcaacg atgggaacgc tcgaagtcag atcgagctac ggctattggt
    10981 tactcctcgt gcaccagggt ttccactaaa gaattggggc ccagccacga cgtagctagg
    11041 ggacgcccta gtggggtttt cctccatccc gctgcaccct gactccaaca gccaacgaac
    11101 attggtttgg aatgttgtgt gaggatgatc atggggatcg ttgagccttc tgtgaacctg
    11161 acgtcggtgt tgcctaatca cacaccgctg tgtccgaagt tgaattgtaa tggaacaatg
    11221 aaaccggact ttaggatggg agcgacgaca ggcttgggac tgtgtgccct ggtgtgtcca
    11281 gtctgcggac atagcggata tcgatcgtgt gatggcattc gtgtgttatt tgcgaccagg
    11341 catgaatatg tttgcagtta tggaccatct ctcaaaagcc tgacagtcgt gttatccggc
    11401 gccgcactga cctcattgcg taagaaaggg ctctgcccaa cgcaatccgc actctttgcg
    11461 gcacattggg cactgttgtg cgggcaactg gttgggacgg tacgtgtctt tcctgagagt
    11521 cccgcttttg tgcacggcta tgcgtacttg caacgtgaga ttgtaggaga cttgtccagc
    11581 cattcaactg acacatttcc cgagacagaa gcttcggtcc ttcaggatgt cacatgcgtt
    11641 gagcgcgcac ggggagatgt tcgttctcga cgaactgaga cattcagggt ggaatgagga
    11701 aataacgcat cacccttctt ccgccaattc cataccaaca ccttcggcaa ggaggggcag
    11761 gatgctggca cggatgcttc gttccctggc tctcgtgacg tgcatagcgg gaggggctgg
    11821 aggctgtctg ctatcccagt ccggttcctc gactacattg cccagcagcg cgtttttttc
    11881 catcgagccg gatcaattag gggctcttca agagatcggc catgctcaag acctgctcat
    11941 gaaacagtgc cataaaggag cgacctgtga ggaggcgtat tatatccgtg gcttagtagc
    12001 gctctttgag aatcgagcga acgccatttc agtgtttcaa gagttgcata ccgtcatgcc
    12061 gaatagtcgg tatgactccg ccacgacagg ttggttgaat cttttgcagg accgcgcctc
    12121 gtcctccagc gagagcaagg ccgtgctggg gcagctgaaa cagcaagtgt tgttgaagtt
    12181 gctggagcat cggggcggta tagtggcgca aggtacggaa gcgcagaaca ggcgaatcgc
    12241 agagctcaat gaatagccgc tccccgcgtg cactctatat gattctggcg ggttcagggt
    12301 cccagctgtc aaaatcgcga tgctgacgca gcggtatgcg cgccattatc cggaaagttt
    12361 gcgggatggg atggcgattt tcgatgcggg aaggacgttt agcacaaatt t
//
LOCUS       DAOQEW010000111        12272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1504_length_12272_cov_7.877548, whole genome shotgun sequence.
ACCESSION   DAOQEW010000111 DAOQEW010000000
VERSION     DAOQEW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12272
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1504_length_12272_cov_7.877548"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..>1570
                     /locus_tag="PLS35_12325"
     CDS             150..>1570
                     /locus_tag="PLS35_12325"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNI19498.1"
                     /translation="MTTMADAMVLTVPPERLRDPNAFLAREWLVTNGLGGYASQSLLC
                     APTRRYHGLFVPDLPAPWGRTVMMPRFDDEVLVDGDAVFLSGVEFSDGRLESNLLSVF
                     DGFSRERQTPVWRIRVKGRRLEKRVIMPYGHNSVYVEYRLLEGDPVGLRLRPFVTFRM
                     LDARLHDAKRPPFPLTVLNGRYDMHLCEGAPSLKLCLRPHGGKFVADYELSVDVSYRV
                     DRDRGSEHIESLASPGYFVADLTQDRPLAFVASTEPWELLEFDPEAIFEAEAQRLDKL
                     LSQRGGEADEDIEGWLTMAADQFVVAPGSRLEEQALARASGNEARTIIAGYHWFTDWG
                     RDTMISLEGLTLCTGRHQEARAILRTFANYVKDGLIPNLFPEGERMGLYHTADATLWY
                     FHAISRYYRVTNDRETLLTLLPVLRSVLEHHVRGTHFGIGVDAKDGLLRASAEGYQLT
                     WMDALVDGWVVTPRRGKPVEIQAL"
     assembly_gap    1571..1670
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1671..1862
                     /locus_tag="PLS35_12330"
     CDS             <1671..1862
                     /locus_tag="PLS35_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNI19499.1"
                     /translation="DKSKARGMLDGFRSHLLDDGIGTVSEIFDAEAPFHPRGCIAQAW
                     SVAEVLRAYRNTRAEKERV"
     gene            1875..2597
                     /locus_tag="PLS35_12335"
     CDS             1875..2597
                     /locus_tag="PLS35_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNI19500.1"
                     /translation="MARIGTSGWHYAHWRGPYYPPSVPPRAMLDYYARDLDTVEINNS
                     FYRLPPSGTFEGWKKSTPPGFCFAVKASRYLTHQKKLLDPSPALKRLLGAAVHLGSKL
                     GPILFQLPPHWRCNIERLSDFLRTLPPSHRYSLECRNATWHTGEVYRLLEQHNVAFCL
                     FELGGCMTPIEVTADFVYVRLHGPGNKYQGDYPTATLKAWCRRIRQWTRCDKDVYVYF
                     DNDQAGYAIKNAMSLRAMVDGS"
     gene            complement(2646..4013)
                     /locus_tag="PLS35_12340"
     CDS             complement(2646..4013)
                     /locus_tag="PLS35_12340"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI19501.1"
                     /translation="MEMEALQIPMLYGRVAIAVAALAHALCATFIVGSSVIGSATETI
                     GHVTQNLRFERLSRLIAFTLILTTAGISFLGVTFVFLLNIFWPRFWSQLFHIMFWPLL
                     LEASFFLAEAIFAYTWYYSWDWANQQPARKRWHLVFGWSAAASSLIAMLMIDMVASYM
                     LTPRPPDDTWAKLFNPTMLYLDTHRIVGNLTWTGFGLAGLCAAGFLRSGGEDDRAFYR
                     WAGGVCFIIGFGALLIMPAIGYQYLLRVRHIEPQAFYTLMLGARSWLFDLVATLYSLL
                     VLFGSWYIWRSVRAQPANDEGGTARLALPMSLLIVAIAGMILSQPYQLQHIPGMQILT
                     DRLINPLGKMQPNKYFALAFLVVFGLMNWLFFFRSYANRVPWWQGQSGSEKERSSPAL
                     LITLSLCAMLMMLTMGWARETARAYNGYLIYGQVRLEDERSTYEGMKTNPGTPDAATR
                     RPVSP"
     gene            complement(4013..5230)
                     /locus_tag="PLS35_12345"
     CDS             complement(4013..5230)
                     /locus_tag="PLS35_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19502.1"
                     /translation="MIDYGTVLPAIQIPLLGNSAAVGLFSLLHIVPAALSVAFMLMAP
                     VFEFRGRTDPFDRELALLVTRFTVVVFSVSTVLAVIMVELMIGLFPVTTMWMWNHFRV
                     PILFGVASFMLMLLALYPYYHFWDRLRATSITLHMILGALAAFFMLPWVFMLDGMGAF
                     MLTPVQEGGTWERLFTPTWLPLVIHRMVGNLMIAGYTLAAYGAWRVWSPGEPALKPYY
                     QHLLSRGWVIGLVAFLLQPFTGLLYAMAIHRAAPDAQASIMTGPYVPWLYLQLLLLSL
                     FFLGNDVLTKRMRTLTGNRQRWLDIGFPVLAALLLLSAGYPELRRVCVYLLVGVTVWR
                     VAASVSKAPTESALSPSPVRSLAVGLALLSFLLYLTMGVMREASRRPDTVRGIISLQD
                     ELRHQARMEGALR"
     gene            complement(5223..6257)
                     /locus_tag="PLS35_12350"
     CDS             complement(5223..6257)
                     /locus_tag="PLS35_12350"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNI19503.1"
                     /translation="MSRTQPCEGLIALFPLDADLDSVRLSLEGSGISQDQIALLTPVP
                     LKSASATDGTSQPLWPYIITIVAGLVGIGVGVFFAAGTALLYPLMTGGKPIVSAPIVG
                     IISYETMMLLAIVVTFITMIVRIRRARRASVDRETRIDDGKIALSVRLVEDGQEQRVK
                     TLLEMAGAEDVRLVTHPSPIQQGMQRAHVTAAWLLICITVGGCSPDMQEQPSYRSQEA
                     PRLHSPTGSVPRDSRAILSREARTMELEGGRAPELYRINCLPCHGEGGEGTGLVAPYL
                     HELPANLRSPRVRALSKTELYRIITQGKDMMPSFQGELSATERMILASFVHAMEPDTT
                     MAHEERRSHD"
     gene            complement(6208..7569)
                     /gene="nrfD"
                     /locus_tag="PLS35_12355"
     CDS             complement(6208..7569)
                     /gene="nrfD"
                     /locus_tag="PLS35_12355"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNI19504.1"
                     /translation="MNHSTAGWEEMPAINRDLLAPLSAGGRGYVLLVTLLAAMVSAGI
                     GAWVYQIETGIGRTNLHPPIFWSAYIASFVYWIGVSHSGTFISGVLRLTKAEWRRPIT
                     RVAELMTIISVVIATLFVFYHLGRSWRWYYLIPYPNQREIWPDFRSPLMWDATAVFTY
                     ATASTIYLYLPLIPDFALVRDRIGGWRKPLYSLLSLGWRGSQREWTALNTAIRIITPL
                     IVMVMISVHSIVGWDFGMSLVPGWHTSIIAPYFVVGAVHSGLGMVVIGLYLVRRIYRL
                     DAYIRLEHFDKLGKLMIVMTLTLAYLYFADQLTVWYGAIPDHLSVMHAVMSGPYAVPQ
                     WTMITLIYVLPLITLTIPAFRTWPAGMLVIGLCINIGMYIERMLILTPPLAHPRLTYN
                     WSDYFPSWVELTIMAGSLSLAVLLYVLAIKFVPIISIWEEKEGRLHEQDSALRRPDRT
                     LSA"
     gene            complement(7566..8288)
                     /locus_tag="PLS35_12360"
     CDS             complement(7566..8288)
                     /locus_tag="PLS35_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNI19505.1"
                     /translation="MEEKQRPSDEPRPYRWQMVIDLDRCTGCEACVVACHAENNIRIS
                     GPGEASDGRAINWIRIERYWEGAYPDVKARFMPVLCQHCADAPCEPVCPVYASYHTPE
                     GLNAQVYNRCIGVRYCGNNCPYTARQFNWFDPHWDPPLEQQLNPDVTVRQNGIMEKCT
                     FCVQRIRQGKEQAERDGRPVRDGDITPACAQSCPTTALVFGDGNDPTSRVATMAKSGR
                     GFHLLESLGTHPAITYLKRLDE"
     gene            complement(8341..10557)
                     /locus_tag="PLS35_12365"
     CDS             complement(8341..10557)
                     /locus_tag="PLS35_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNI19506.1"
                     /translation="MDVKRRTFIKALGLTAAGTLLPGCDQEVRRLVPYVLPDDEIVPG
                     VAEWYASTCGECEAGCGIIVKVMEGRAKKIEGNPLHPVNQGKLCAQGQAALQRLYNPD
                     RLRGPLRRMGTEADQRFTPVSWDEGLHQLADQIRHAKGPVIMISRPLSGTLADVVSDF
                     VASVKGSLYFYDPSPELALRAAMKQAFDIHDLPAYDLGDADYVLSFGAPFLEQWLSPV
                     SFGIAFGRMRQGRKNRRGRFVHVEPRLSLTAASADRWLPIRPGMEGLLARGIGHILLS
                     EHRSSLPTGRLHAYESLYGRLSLDEVAKQTDISTADIKRVARAFAEADSPQAIGGGTA
                     CAHTNAFETHLAIHGLNALAGNINKAGGIRWYQSASLTLHPEIPWLSERRIKELAETI
                     EKTGQRPLLLLYDCNPLFSVPPAIPIRRLFDRASYIASFSGSLDESTVSADLILPDHH
                     RLETWGDHVQAGSSPYRAASLVQPVVVPLYDTRGLGDTLLELTRQLAGKHAGVESFPE
                     HIKKRWQHFHTSHSSGSHEPFQSVWEQLLQQGGWWDSAANEQRLQQSSSAVEYRPPEF
                     DGDASEFPLYLYPYAAPHLGQGRGANLPWLQELPDTMTSAVWGSWVEINPATAQAMGI
                     QQGALVRLHSRHGSLEAPALHYPGLRPDVIAMPIGQGHTDYGRYAARRGANPMTILAP
                     LFDKQSGALAIGATRVRAEPLNVPGTLILLEKPGTTAAGSSDLISIERPPSSKART"
     gene            complement(10595..11113)
                     /locus_tag="PLS35_12370"
     CDS             complement(10595..11113)
                     /locus_tag="PLS35_12370"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNI19507.1"
                     /translation="MTRSLWQSLGLVSLATVSALAIGILYSKGSADSAAHDQQPIAFN
                     HAFHAGELALDCRYCHRAAHISPAAGIPSLQLCMGCHQNLDVATAGIQTLVDHWKRQA
                     PVEWVRLQRLPDFVYFTHERHLAQGLECTTCHGDVTRMTSTPRAATYEMGWCLSCHQQ
                     RGASRDCVTCHK"
     gene            11394..>12272
                     /locus_tag="PLS35_12375"
     CDS             11394..>12272
                     /locus_tag="PLS35_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNI19508.1"
                     /translation="MERDVCSTNGDSGPKIEKVQVSAYTIPTAGPESDGTLEWDRTTL
                     VLVDVSAGGQHGIGYTYADVSTAWFVKQNLAPVVEGRDAMHIADAWWAMMRSVRNLGH
                     PGVASMAIAAVDTALWDVKARLLDLPLVSLLGRVRTAVPVYGSGGFTSYSIDRLEEQL
                     GGWVRDGISRVKMKVGRQPDQDLRRVEAVRKTIGEEAQLFVDANGGYGRKQALQLSEG
                     FAKAGVVWFEEPVSSDDTEGLRLLRDRVPAPIQVTAGEYGYDLFYFRRLLDAEAVDVL
                     QADASRCGGITGFLKVG"
BASE COUNT         2600 a         3419 c         3785 g         2368 t
ORIGIN      
        1 tacggaggac cgggagtcat cgagccgttg accgaaaccg gctggaaaat tcccgggggt
       61 gcggccttcc tgtacgccgc tctggacgat acatccaagg acactggcaa gactcccgcg
      121 atggcgagcc cgcatgaaga ggaatcgcga tgacgacaat ggccgatgcg atggtgctga
      181 cggtccctcc ggagcggctc cgtgatccta acgcctttct cgccagagaa tggctggtga
      241 cgaacggtct cggcggctat gcctcgcaaa gcctgttgtg cgctcccaca cggagatacc
      301 acggtctgtt cgtgcctgat ctgccggccc cttgggggcg cacggtcatg atgcctcgtt
      361 ttgacgacga agtgctggtc gatggcgatg cggtcttttt gagtggtgtg gaattttccg
      421 atggccggct ggaaagcaat cttctgtcgg tattcgacgg cttcagccgg gaacggcaga
      481 cgccagtctg gcgcatccgg gtcaaaggcc gccggctgga aaagcgcgtg atcatgccct
      541 atggccacaa ctccgtctac gtggaatacc gccttcttga gggtgatccg gtcggcctgc
      601 ggcttcgccc gttcgtcacc ttccggatgc ttgatgcgag attgcacgat gccaagcgac
      661 cgcctttccc tctcaccgtc ctgaatggcc ggtacgacat gcatctgtgc gaaggagcgc
      721 cttcgttaaa attgtgcttg cgtccccacg gagggaagtt cgtggcggat tacgagctga
      781 gtgtcgatgt gtcctaccgg gtcgatcgcg atcgaggctc ggagcatatc gagagcctcg
      841 ccagccccgg ctattttgtg gcggacctta cgcaggatcg gcccctcgcg tttgttgcca
      901 gcacggaacc gtgggaactg ctcgaattcg accccgaagc catttttgag gcagaggccc
      961 aacgcctgga taaattgctg tcccaacggg gaggcgaagc ggatgaagac atcgaaggtt
     1021 ggctgaccat ggcggccgat cagtttgttg tcgccccggg ctctcgactg gaggagcagg
     1081 cgctggcgcg tgcctccgga aatgaagcgc gcacgattat cgcggggtac cattggttca
     1141 cggattgggg ccgcgatacg atgatcagct tggaaggtct cacactctgt accgggcgcc
     1201 atcaggaagc ccgagccatc ctccgaacct ttgccaatta tgtgaaagat ggattgattc
     1261 cgaatctctt ccccgaagga gagcggatgg gcctctacca cacggcggat gcgacgctgt
     1321 ggtattttca cgcgatcagc cggtattacc gggtcacgaa cgaccgtgag acgctgttga
     1381 ccttgttgcc cgtcctccgg tccgtgcttg agcaccatgt tcggggtaca cacttcggaa
     1441 tcggtgtgga tgcgaaagat ggactcttgc gcgcctcggc ggaaggttac caactgacct
     1501 ggatggacgc cttggtggat ggctgggtgg tgacaccccg tcggggaaaa ccggtcgaga
     1561 tccaggcgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gataagtcca
     1681 aggcgcgagg tatgttggat gggttccgtt cgcacctgct ggatgacggg atcgggacgg
     1741 tcagcgaaat ttttgacgca gaagctccct ttcatccgcg gggctgcatt gctcaggcct
     1801 ggagcgtcgc cgaggtgtta cgagcctatc gaaacacgcg agccgagaag gagagggtgt
     1861 gagggattgt gggtgtggcg cgtatcggta cgtcgggatg gcattacgca cattggcgcg
     1921 gaccctatta tccgcccagt gtcccgccgc gagcgatgct ggattattac gcccgtgatc
     1981 tggataccgt cgagatcaac aacagttttt accgcctgcc cccatcgggg acgtttgagg
     2041 gatggaagaa gtcgacgccg ccgggttttt gtttcgcggt caaagccagc cgctacctca
     2101 cccatcagaa aaaactgctc gatccttcgc ctgcgctgaa acgattgctg ggtgcggcag
     2161 tgcatctcgg ttcaaaattg ggcccgattc tgtttcaatt gcctccccat tggcgatgca
     2221 acatcgaacg gttgagcgac tttctgcgca cgctgccgcc ttcgcaccgg tatagcctcg
     2281 aatgccggaa tgcgacctgg cacacgggcg aggtctaccg tcttctggaa caacacaatg
     2341 tggccttttg cctcttcgaa ctcggtggat gcatgactcc catcgaggtg acggcggatt
     2401 tcgtctacgt gcggctccac gggcctggca acaaatatca aggcgattat cccacggcga
     2461 cgctaaaagc ctggtgccgt cgcatccggc aatggacccg ttgcgacaag gatgtgtatg
     2521 tgtatttcga taacgaccag gccggttatg cgatcaagaa cgcgatgtct ctgcgggcga
     2581 tggtcgatgg ttcttaattg agtgattgct gaggcgagcg attggcggac gtgaaatatt
     2641 ggtcgctagg gcgaaactgg acgacgggtt gccgcgtcgg gcgtgcccgg attggtcttc
     2701 atcccctcat aggtgctgcg ttcatcttcc agtcgcacct gtccatagat caagtagcca
     2761 ttgtatgcgc gcgcggtttc acgggcccac cccatggtca acatcatgag catcgcgcat
     2821 agggataacg tgatcaggag tgcgggactc gatcgctctt tctcggagcc gctctgaccc
     2881 tgccaccatg gaactcgatt ggcataggat cggaagaaaa agagccaatt catgaggccg
     2941 aagaccacaa gaaacgcgag ggcgaaatat ttgttcggtt gcatctttcc caaggggttg
     3001 atgaggcggt cggtgaggat ctgcatcccc gggatgtgtt gcagttggta gggttgggaa
     3061 agaatcatac ccgcaattgc aacgataagc agcgacatcg gcagtgccag cctggctgtt
     3121 cctccctcgt cattcgctgg ctgggcccgt acgctccgcc agatgtacca ggatccgaac
     3181 aggacgagga gtgagtagag ggttgcgacg agatcgaaga gccaggagcg ggcgccgagc
     3241 atcagcgtgt aaaacgcctg cggttcgatg tggcggactc tgagcagata ttggtacccg
     3301 atggccggca tgatgagcag ggcgccgaag ccgatgatga agcagacccc tcctgcccac
     3361 cggtaaaacg cgcgatcgtc ttcgcctccc gaacgtagaa atcccgccgc gcagaggccc
     3421 gcgagaccga atccggtcca ggtcagattg cccacgatcc gatgcgtgtc cagatagagc
     3481 atggtgggat tgaacagttt cgcccaggtg tcatcgggtg ggcggggagt caacatatag
     3541 gaagcgacca tgtcgatcat caacatcgcg atgagggaag aggcggcggc ggaccacccg
     3601 aagacaaggt gccagcgttt gcgcgccggc tgctggttcg cccaatccca ggagtaatac
     3661 caggtgtagg caaagatggc ctccgccaga aagaatgatg cttcgagcag aaggggccag
     3721 aacataatat gaaacaactg actccagaat cggggccaga agatgttcaa gaggaagacg
     3781 aatgtcacac cgagaaagga gattccggcg gtcgtgagaa tgagtgtgaa ggcaatcagg
     3841 cgggagagcc gttcgaagcg aaggttctgc gtgacatgcc cgatggtttc cgtcgccgag
     3901 ccgatcaccg aggagccgac gatgaaggtc gcgcagagcg catgggccaa agctgcgact
     3961 gctatagcca cccgtccgta gagcatggga atctggagcg cctccatctc catcagcgga
     4021 gcgctccttc catgcgtgcc tgatgccgca attcgtcctg aagcgaaatt atgccgcgga
     4081 cggtgtccgg tcgccgggag gcttctctca tgacacccat ggtgaggtac agcagaaagg
     4141 ataagagagc caggccgacg gccagtgagc gcaccggtga cggagagaga gccgattctg
     4201 tgggtgcctt ggacacggag gcggccactc gccataccgt cacgccgacg agcaggtata
     4261 cacagacccg gcggagttcc gggtagccgg cagagagtag gagtagcgct gccagaacag
     4321 gaaagccgat gtctagccag cgttgtctgt tgccagtcag cgtccgcatg cgtttcgtca
     4381 agacatcatt gccgaggaag aacagagaga ggaggagcaa ttggagatag agccatggca
     4441 cgtatgggcc ggtcatgatg gatgcctggg cgtccggtgc tgcgcgatgg atcgccatcg
     4501 catacagcag gcccgtgaag ggctgcaaca ggaacgcgac caatccgatc acccagcctc
     4561 tggagagcag atgttggtag taaggcttga gagcaggctc gccggggctc caaactcgcc
     4621 aggcgccgta ggcggcaagg gtatatcctg cgatcatcag atttccgacc atccggtgga
     4681 tgacgagcgg cagccaggtg ggagtgaaga gtcgttccca cgtccctccc tcctgcaccg
     4741 gcgtcagcat gaaggcaccc atgccgtcca gcatgaagac ccacggcagc atgaagaaag
     4801 cggccaaggc tcccagaatc atgtggagcg tgatactggt tgctcgtaag cggtcccaga
     4861 aatggtagta gggatacagg gccagcagca tcagcatgaa cgaggcgacg ccgaatagaa
     4921 tcgggacgcg aaagtgattc cacatccaca tcgtggtgac cgggaagaga ccgatcatca
     4981 gttccaccat gatgacggcg agcacggtgc tgacactgaa caccaccacg gtgaatcgtg
     5041 tcacaagaag cgccagctct cgatcaaagg gatccgtccg ccctctgaat tcaaaaacgg
     5101 gcgccatcaa cataaaggcc acggaaagcg cggctggaac gatatgcagc aggctgaaca
     5161 agcccaccgc ggcgctattt cccagaagag gaatctgaat ggcaggcagc accgttccat
     5221 aatcaatcat gtgaccgtcg ttcctcgtga gccatggtgg tgtctggctc catggcatgg
     5281 acgaatgacg ccagaatcat gcgctcggtg gcggacagct cgccttgaaa ggaaggcatc
     5341 atgtccttcc cctgcgtaat gatccggtag agttctgttt tggacagagc tcgcactcga
     5401 ggtgaccgca ggtttgccgg cagttcgtgc aaatagggcg cgacaaggcc cgttccttct
     5461 ccgccctcgc catggcaggg caggcaattg atgcgataca gctcaggggc gcgccctcct
     5521 tccagctcca tggtgcgagc ttcccgggac aaaatggcgc gactgtcgcg agggacgctt
     5581 cccgtcggag aatggaggcg cggggcttcc tggcttcgat aggacggctg ttcctgcata
     5641 tccggggaac agccccccac ggtgatacag atcagaagcc atgcggccgt cacatgagcc
     5701 cgttgcatcc cttgttgaat cggcgaaggg tgagtcacca gccgaacgtc ctccgcgcct
     5761 gccatttcga gcaacgtttt gacgcgctgt tcctggccgt cttcgacgag acgcactgag
     5821 agcgcaatct ttccgtcatc gatcctggtc tcacgatcaa cggaggctcg ccgtgcccgt
     5881 ctgatcctga cgatcatcgt gataaacgtg acgacgatcg ccaacaacat catcgtttca
     5941 taggaaatga tgccgacgat gggagcggac acaatcggtt tgcctccggt catcaaggga
     6001 tacaggagcg cagtgccggc ggcgaagaag actcctacgc ctatgcccac aagcccggcg
     6061 acgatcgtga tgatgtaagg ccacaagggc tggctggtac catctgttgc ggaagccgac
     6121 ttcaacggca ccggggtcag gagtgcgatc tgatcctggg agatgccgga tccctccagc
     6181 gacaggcgca cagaatcgag atcggcatca agcggaaaga gtgcgatcag gccttcgcaa
     6241 ggctgagtcc tgctcatgca accgtccttc cttttcttcc cagatggaaa tgatcggaac
     6301 gaacttgatc gccagcacgt agaggagcac cgccagcgaa agggaaccgg ccatgattgt
     6361 cagttcaacc cacgacggaa aatagtcgct ccaattgtag gtcaaccggg gatgggccaa
     6421 cggcggggtg agaatcagca tccgctcgat gtacatgccg atattgatgc agaggccgat
     6481 caccagcatg ccggccggcc acgtgcggaa ggcgggtatt gttaaggtga tcagcggcag
     6541 tacatagatc aacgtgatca tggtccattg aggaacggca tagggtccgc tcatgactgc
     6601 atgcatgacc gacagatgat ccggtatcgc tccgtaccag accgtcagct ggtctgcgaa
     6661 atagaggtag gccagcgtca gcgtcatcac gatcatgagc ttgccgagtt tgtcgaaatg
     6721 ttccaggcga atgtaagcgt cgagtcgata gattcgccgc acgaggtaca aaccgatgac
     6781 gaccatgccc agtcctgaat gcaccgcgcc caccacgaag tagggggcga tgatcgacgt
     6841 gtgccatccg gggaccagcg acatgccgaa gtcccagccg acaatcgaat gtaccgagat
     6901 catgaccatc acaatcaggg gagtgatgat cctgatcgca gtgttcagcg ccgtccattc
     6961 cctctgggaa cctcgccagc cgaggctgag taaggaatag agcggcttgc gccatccgcc
     7021 gatgcgatcg cgaaccaatg caaaatccgg aatcagcgga aggtacagat agatggtgct
     7081 cgcggtggca taggtaaaga cggcggtggc atcccacatg agcggagaac ggaagtccgg
     7141 ccagatttca cgctggttgg gatagggaat caggtaatac caacgccagg atcttcccag
     7201 gtgataaaag acgaacaggg tcgcaatgac cacagagatg atcgtcatca actcggcgac
     7261 cctcgtaatc gggcgtcgcc attccgcctt ggtcagtcga agcaccccgg agataaaggt
     7321 gccggaatgg ctgacgccga tccaatagac gaaggaggca atgtaggcac tccagaagat
     7381 gggaggatgc aggttcgtcc ggccgatgcc ggtttcgatt tgatagaccc aggctccaat
     7441 gccggcggat accatggcgg ccagcaatgt gaccaggagc acatacccgc ggcctcccgc
     7501 tgaaaggggc gccaacaggt ctcgattgat ggccggcatc tcttcccagc cggctgtgct
     7561 gtggttcatt catccaaccg tttcaaatag gtaatggccg gatgcgttcc gagcgattcc
     7621 agcaggtgga agccccgtcc gcttttggcc atggtcgcaa cccggcttgt agggtcattg
     7681 ccgtcgccga ataccaacgc ggtggtcggg caggattgag cgcaagcggg ggtgatatcg
     7741 ccatcgcgca ccgggcgacc gtcgcgctcc gcctgttcct taccctgtct gattcgctgc
     7801 acgcagaacg tgcatttttc catgatgccg ttttgccgga ccgtcacgtc gggattgagt
     7861 tgctgttcca acggtggatc ccagtgggga tcgaaccagt tgaattgccg cgccgtatac
     7921 ggacaattat tgccgcagta gcgcaccccg atgcatcggt tatagacctg ggcgttgagg
     7981 ccctcagggg tgtgatacga cgcgtagacc ggacagaccg gctcacaagg cgcatcggcg
     8041 cagtgttggc agagcacggg catgaacctc gctttgacgt cgggatatgc gccttcccaa
     8101 tagcgttcga tgcggatcca attgatggcg cggccgtcgg acgcttctcc ggggccggaa
     8161 atgcggatat tattttcagc atggcaggcg acgacgcagg cctcgcagcc ggtgcatcga
     8221 tccaggtcga tcaccatctg ccaccggtaa gggcggggtt catctgatgg cctctgcttc
     8281 tcttccatgc ttcagtcctt tcgtcgaaac ctccggttca cccagtgaag gccgtccgac
     8341 tcatgtgcgg gccttggatg agggcggccg ctctatcgag atcaggtcag agcttccagc
     8401 cgcggtcgtg cccggcttct caagaaggat caaagtcccc ggaacgttca acggttcagc
     8461 ccggaccctt gtcgcgccga tggccaaggc ccccgattgc ttgtcgaaca ggggcgcgag
     8521 aatcgtcatg ggattggctc cccgccgagc ggcatatcga ccatagtccg tatggccttg
     8581 gccgatgggc atcgcgatga cgtcgggccg cagcccggga tagtgcaggg caggcgcctc
     8641 gagagatcca tggcgagagt gaaggcgcac gagtgcaccc tgctgaatcc ccatggcctg
     8701 cgccgtggcc ggattgattt caacccagct tccccatacg gcgctggtca tcgtgtcggg
     8761 tagttcctgc agccagggca gattcgcgcc gcgtccctgc cccagatgcg gcgcagcgta
     8821 ggggtatagg tacaagggaa actcggaggc atcaccgtca aactctggcg ggcgatactc
     8881 cacagcggaa gacgactgct gcaagcgttg ttcgttggcc gccgagtccc accagcctcc
     8941 ctgttgaagg agttgttccc agactgattg aaacggttca tgggagccgg acgagtggga
     9001 cgtatgaaag tgctgccagc gtttcttgat gtgctcagga aacgactcga ccccggcgtg
     9061 cttgcctgcc aattgacgtg tcaactcaag cagcgtgtcg cccaacccgc gcgtgtcata
     9121 cagcggcacc acaaccggct gcaccagact cgctgcacgg tacggcgagg acccggcctg
     9181 aacatggtcg ccccaggttt ccaaccgatg atggtcaggc aggatcagat ccgcactgac
     9241 ggtggactca tcgaggctgc cgctgaaact ggcaatgtag gaagcgcggt caaatagacg
     9301 tcgaataggg atggcaggtg gaacgctgaa caagggatta cagtcataga gcaacaagag
     9361 cgggcgttgc ccggtcttct cgatggtctc cgccagttcc ttgatgcggc gttctgacag
     9421 ccaggggatc tcgggatgaa gggtaagtga ggccgactga taccagcgga tgcctcccgc
     9481 tttgttgatg tttcctgcta aggcattgag cccgtgaatg gccaggtggg tttcgaacgc
     9541 gttcgtatgg gcacaagcag tgccgccgcc gattgcctgg ggagagtccg cctcagcaaa
     9601 tgctcttgca acccttttga tgtcagctgt ggaaatgtcc gtctgcttcg ccacctcgtc
     9661 cagagagaga cggccataca acgattcgta tgcatggagt cgacccgtgg gcagactgga
     9721 acggtgttcc gaaagcagga tatgaccgat accgcgcgcg agaagccctt ccatgccggg
     9781 ccggatgggc agccaacgat ctgcgctggc ggccgtcaga gacaggcgag gttcgacgtg
     9841 aacgaaacgc ccgcgtcgat tcttgcgtcc ctgtcgcatc cggccgaacg cgataccgaa
     9901 cgacaccggc gacagccact gttccagaaa cggtgcgccg aatgacaaca catagtccgc
     9961 atcgcccagg tcataagcag gaaggtcgtg aatgtcgaat gcctgtttca tcgccgctcg
    10021 cagagccaat tcaggcgacg gatcatagaa gtacaaggag ccctttacgg aagcgacaaa
    10081 gtccgagacg acgtctgcca gcgtgccgga gagcggccgg ctgatcatga tgaccgggcc
    10141 cttcgcgtgg cgtatctggt ctgccagttg atgaagaccc tcatcccacg aaaccggcgt
    10201 aaatcgttga tccgcttccg tgcccattcg gcggagggga ccccggaggc ggtcagggtt
    10261 gtaaaggcgt tgaagcgctg cctgtccctg cgcacagagc ttcccctggt tgaccggatg
    10321 caaaggattg ccttcgattt tttttgcgcg gccttccatt accttcacga tgatgccgca
    10381 gccggcctcg cattctccgc aggtcgaggc ataccactcg gccacacccg gaacgatttc
    10441 gtcgtccggg agcacgtacg gcaccaatcg gcgaacctct tggtcgcacc cgggaagaag
    10501 tgtcccagct gccgtcagcc ccagtgcttt gataaacgtt cgccgtttga catccattcg
    10561 aatcccacat ccatatgatc aacgcggcag ccgatcattt gtggcaggtc acgcaatcgc
    10621 gtgaagcccc acgttgctga tgacacgaga ggcaccagcc catttcatac gttgccgcgc
    10681 gtggtgtgct cgtcatacgc gtcacgtcgc catggcatgt ggtgcattcg aggccctggg
    10741 ccagatgacg ttcatgggtg aaatagacaa aatccggcag acgttgcagt cgcacccact
    10801 ccaccggtgc ttgccgcttc caatgatcga ccaatgtctg gatgcctgct gtcgcgacat
    10861 ccaggttttg gtgacaaccc atgcacagct ggaggcttgg aatcccggcc gccggagaga
    10921 tatgcgcggc cctgtggcag taccggcagt ccagggcgag ttcgccggcg tggaaggcat
    10981 gattgaatgc aattggctgt tggtcgtggg cggcggagtc agcgctgcct ttcgagtaca
    11041 gaatcccaat ggccagcgcg ctgacagtgg ccagcgagac cagaccaaga ctttgccaca
    11101 atgaacgtgt catcggacgg caaggaacat tctcgtagcg cgttccaact cgttgttgtc
    11161 tcatctattc agaggctgag agacggttct actaggcatt cctcctgagc ccgcgtcgaa
    11221 taactaccga agcctctctg ctagggaacc ctctagctca aagcctcgtc cgccagagct
    11281 acagtggggg gacacgtctc gacggtatga gaaccgtcga aggctcgaat ctttcacaaa
    11341 cttcacacgg tctaaagatg cctccacaga ctagagatga ggaacgacgt gtgatggagc
    11401 gagatgtctg ctcgaccaat ggcgactccg gcccgaaaat cgaaaaggtt caggtctctg
    11461 cctacacaat tcccacggcc ggcccggaga gcgatggcac actcgagtgg gatcggacga
    11521 cgctggtgct cgtcgatgtg agcgcagggg gccagcacgg catcggttat acctatgccg
    11581 atgtctcgac cgcgtggttt gtgaagcaga acctggcgcc tgtcgtcgag ggtcgtgatg
    11641 cgatgcacat cgcggacgcc tggtgggcca tgatgcgctc agtgcggaat ctcggccatc
    11701 cgggagtggc ttcaatggcc atcgcagcgg tggacaccgc cttatgggac gtgaaagcac
    11761 ggctgctcga tcttccgttg gtgtcgctgc tgggccgcgt ccggacagcg gtgccggtct
    11821 atgggagcgg tgggttcacc tcttattcga tcgatcggtt ggaagagcag ctaggtgggt
    11881 gggtcaggga cggcatttca cgcgtcaaaa tgaaagtcgg ccgtcaaccg gatcaggatc
    11941 ttcgccgggt ggaggctgtg cggaagacga tcggagagga ggcacaactg tttgtcgacg
    12001 ccaacggcgg ctacggccgg aaacaggcat tgcagctgtc cgaaggcttt gccaaggccg
    12061 gggtcgtatg gtttgaagag ccggtctcgt cggacgacac ggaggggctg cgcttacttc
    12121 gtgatagggt gccggcgccg atccaggtga cggcagggga atacggatac gatctcttct
    12181 attttcgccg cctgctcgat gcggaggccg tcgacgtgct gcaagcggat gccagccgct
    12241 gcggaggcat cacgggattc ctcaaagtcg gg
//
LOCUS       DAOQEW010000112        12152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1530_length_12152_cov_8.356948, whole genome shotgun sequence.
ACCESSION   DAOQEW010000112 DAOQEW010000000
VERSION     DAOQEW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1530_length_12152_cov_8.356948"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1722)
                     /locus_tag="PLS35_12380"
     CDS             complement(259..1722)
                     /locus_tag="PLS35_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNI19509.1"
                     /translation="MSTIRFCTAPASSETDPSSASRETVAPGRNARTTARLLLLTGAL
                     LAAWSTIAPGSVLAGSFRIYDHSASATGQASAFTAQADDASAGYYNPAGMTQLRGVQL
                     SAGTTLIAGGFSFQNGAGTQANGDLRGSVAVPPPSNLYITANLKDLGIGSSDSTAVGL
                     AVFSPFGTLTRWPDSSPFSTATTKAAFELIDIRPSIAFRPLPDLAIGLGADVYTFSGA
                     FGEGQVERQFRWPGGLGIPAGTGMELNGRDTAAGWNASLLYTPLRNEDGKPLVNIGLI
                     YRSQATLHLDGQLLAGGTHIADARTTFVVPQVLTGGIALWPVRNKEREWKLELDVDYT
                     GWKSVRNLDSHLSNGITVATPANWISGYTVMIGTEHKWLNPASLPDWDIALRAGYWHS
                     QKAIPDQTFNPAIPDGDNHAVSTGVGFMCRDNGRFLGVIPCGGASKLSPKGLGIDLAY
                     QAILYESRTVAGNVNPTVNGTYRTTLHVGSLNLRVNF"
     gene            1902..4199
                     /locus_tag="PLS35_12385"
     CDS             1902..4199
                     /locus_tag="PLS35_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNI19510.1"
                     /translation="MRFVRRWLLRALVAVTGILLLVLGSALAYGLYLSASLALPAGDE
                     HPPRLIYGAPFLLKPDLDVVSSHLIERLSRLGYQAVDTPVRAPGEFRVSPGQIDIYLH
                     EFSDLHLRPMPARLLLEQGRVTRVLSIQTGDELFPAYLEPQLISGLRGASRQVREWVS
                     YDDLSARFLDILLAIEDRRFFSHPGIDPIAVGRAVWTNVTKGTVIQGGSTITQQLAKN
                     LFYSPQRTFGRKLKESIAALVLETKYPKKAILESYANEIYLGQVGSVSIYGVGEAAHR
                     YFGKRVDTLSLEEAALLVGMIKGPNTYSPLRNAALAKQRRDVVLGRLHEQGLINDEAW
                     AQAIKTPVQVMPPQDTLADAPFFVDYLLRQVEEATGSPLPDGVRAYTTLDPVLQRLAT
                     QTLESELIKLETTYPALKGHPTPLQGALVAIDPKTGGILAMVGSRDYRTSQFNRAVQA
                     KRQPGSLFKPLVYLAAMEARRELNGGQPVTAATSIVDEPVTFESATGRWTPQNYDHKF
                     HGTVSLRTALEQSLNIPAVKIAQLVGTKRILSLAEKLGIRSPLADNLSIALGTSGVSL
                     LEMTSAFGVLAHDGVSMTPTALQAVMTSEGDPAWHHTPARHQAVSPQAAYVMTSLLKG
                     VVERGTAAKAKAMGLRGIVAGKTGTTDGYRDAWFIGYTPDVVVGAWVGFDDEDALHLT
                     GAQAALPMWVEFVRQILPAAPRDFVMPPAVITRDIDPQSSQLATSKCPQRSAELFIEG
                     TEPSVYCEVHGSGMWERVKRSFGFF"
     gene            4286..4726
                     /locus_tag="PLS35_12390"
     CDS             4286..4726
                     /locus_tag="PLS35_12390"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNI19511.1"
                     /translation="MMKKPGFVESDNITLLAKGVLLRGEIHVEGTVRIDGRLEGDLKT
                     SGTVVIGEDGVVQGTITAGTIISSGKIKATVRATERIQLLKTGLLVGEVHAPAFSMED
                     GAKFQGLSDMGVTSWGDDAQKLPGNVREIASQRPKAVAMAGENA"
     gene            4901..5983
                     /locus_tag="PLS35_12395"
     CDS             4901..5983
                     /locus_tag="PLS35_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNI19512.1"
                     /translation="MNRRQIFAACFFGVLLALLYQMALMFRPFVFPVLWAVILAHLCF
                     PLHIRLTAWLGGKESHSAVLLTLAALALVVVPLVVLSVMLVKEAGSAERAVREWIASG
                     GVQQLPDRLSGLPGGSVAREWLERFTGQESDLEQFVLSSAKTFSKFIVGELSDLVRDV
                     FMLVADFLVMVFTLFFFFKDGRHWLASLYGLIPLEASHKDKILVRLDQTIRAVVKGIV
                     LTAVAQGLLAGAAYAVLGVPFPMVLMALTILLAPLPFGGTALIWGPVAGYLLWSGTVG
                     KGLVMLAWGIGVVSTIDQILKPLFIGQGAQIPVLLLTFSVLGGLAAYGILGLFLGPIL
                     VGLFLTALQIYRDEYQLQPASPIVRS"
     gene            complement(6006..6599)
                     /locus_tag="PLS35_12400"
     CDS             complement(6006..6599)
                     /locus_tag="PLS35_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNI19513.1"
                     /translation="MHFNLTGVRSWTIWMALFLACVSTYSLQAKDLTPPAGISPEKVA
                     DFVHAVLDADRTIYTNQVVNRMQAKGIVSAMEHWEHENALPLPAQFLQHSGKLVAESG
                     RGIRYRLISLWPIYQRNAPATDLERQALESFAQTPDRPFTGIVTSGRKQFFQAVYADR
                     AISAACIKCHNSHPLSPKRDFALNDVIGGMTITIPLD"
     gene            complement(6584..9061)
                     /locus_tag="PLS35_12405"
     CDS             complement(6584..9061)
                     /locus_tag="PLS35_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19514.1"
                     /translation="MTITWRPFSNPLGWGLKRKLIVSMLLVGVVPLLLGLSMAFLQGS
                     KEIQVVSGESFQALATEAARKLDLLVAEEVARTSRIATHPEIIRQLEHHRDLLQTADK
                     TLSATTITQQRTRWAARDPIAVKAITENRTAVLLRSFYAGAQNESDLLLPQVVRAATK
                     KLFITDVQGNLFAALTTFPGFSNADSPWWKGTYNKGVGQLYIEDLHFDEQAKTYVFSV
                     SLPIMDSLKYEVVGVLHRVIDAKEFFSPSTHPIRFGKTGHVMLIDSRGIVLSCPILPT
                     GVRLSDPALIPQVTVREPGWVTADSDGHGGQGTAIIGFALLPETSRATNGALEVGAWH
                     TFVWQSSDELFSPIRHLMLWMLILASVALFLLATLGYFAASRIVTPVRRLQEAARLIG
                     RGELQEPIQIRTGDELEDLAVEFNRMHKQLEASFAGLSTQVEEKTQEVQYLQKATDQV
                     LDAVPTPIIMVDQQGLVQYMNKASREAVQADSWSSRPLFDLLPVDEEHRQALQRDFRA
                     VEEGMSLAPVRHNAPPLLKPARDPLNQTIGPAPASSRRELRIGPHLYQYEWFPLESRS
                     GAGKRFGLVLRDTTDESRLQDQLIQAEKSGSLDVLTAGIGHELNNPLFGILGLGEAIQ
                     EEGDLARAKGYAQDIVGHGRKMAAIIRDFTGVATRESKSQRMPVDVTAQLEQALAIVQ
                     TSHECLGLNVQKHYVPVSPVTALPDQLRLAFINVLTNAIQAMNGQGNLWLTTEEQDDV
                     ITIKIRDNGPGIPKQHLGKVFDPFYTTKGQGEGSGLGLTVAQRLIKKFGGEIRMESPP
                     EGQGTTCVITLPAEKAAVRKEEPCIST"
     gene            complement(9058..10107)
                     /locus_tag="PLS35_12410"
     CDS             complement(9058..10107)
                     /locus_tag="PLS35_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19515.1"
                     /translation="MTLTSTKIAIIGAGRGGTALLELLHQIPDVDIVGIADKDPAAPG
                     LQRAKDLNVPVEEQVYDLIRNHGVNLIMDVTGDPSMAPFIQAMKRPGAEVLSGATTRL
                     LWKLVQHQSKLEAELFQADKLAGLGSFAAGIAHDINNPLQLILGLAENLTDEQDLTVV
                     HEQARDITEAVKRTSAICRDLTRYARRNGTGEDIMVNLNTKLDEALRIARYAVTLQDV
                     TVVKRYAEDASVLGNPDELLHVFVNLITNAVQAIQDRGTLTLHTNVGPDGQVSASVSD
                     TGCGIPKEAFSKIFEPLYTTKPPGKGTGLGLYNVMSVISKMEGHICVESDVGVGTTFR
                     IEFPQVQPTTSCAPL"
     gene            complement(10223..10459)
                     /locus_tag="PLS35_12415"
     CDS             complement(10223..10459)
                     /locus_tag="PLS35_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19516.1"
                     /translation="MLICDIRMPKINGVEAIAYFQQEWPRVPIIVLTGFPDTDMATSF
                     LRSGVVDYLVKPVEGEKLRTAVARAMEQRELAQL"
     gene            complement(10498..10656)
                     /locus_tag="PLS35_12420"
     CDS             complement(10498..10656)
                     /locus_tag="PLS35_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19517.1"
                     /translation="MGEFKSGFFMSETACNGRVLVVDDEPDIRKVVRMTLQKAGYEVI
                     EAETGKKP"
     gene            complement(10941..11303)
                     /locus_tag="PLS35_12425"
     CDS             complement(10941..11303)
                     /locus_tag="PLS35_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19518.1"
                     /translation="MSQTKSRQPRPLVQTTLFVLVLVLGCQATLSVRTASAAGSGSEA
                     IQGLGSYFLTLPYGGIKMAVAVLGAVAGGMGFIFTGGDKATVDKIWGPAIGGEYVVTP
                     QHLRGEKDLHFFGNPPAK"
     gene            11468..11713
                     /locus_tag="PLS35_12430"
     CDS             11468..11713
                     /locus_tag="PLS35_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNI19519.1"
                     /translation="MITAEAVTTYIRQMFPDAAVTVIDKTGTQDHLIVRVISTGFADK
                     NLLDRHRMVYQALAGPMKDGRIHALEITATTPDEVKK"
     gene            11724..12053
                     /locus_tag="PLS35_12435"
     CDS             11724..12053
                     /locus_tag="PLS35_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNI19520.1"
                     /translation="MADPIEEEIQREIQANKILIYGKGTKTMPMCGFTRETMHFFEKY
                     GYPYELIDVLSQPAKREALTKLTNWPTLPKVFIDGQFYGDTDILDPMEAKGEVVPLLK
                     KAFGAES"
BASE COUNT         2337 a         3404 c         3787 g         2624 t
ORIGIN      
        1 cgggaccacg ctccattcca ctgcggcaag aatggcggcg ttgacagcgc tcgctagcac
       61 gcccgtgggc atgttgcggt acagaacaga gacttgttgt gattggagtt ccagtgccgg
      121 atcggtgtgg gccgggcgat ccatgggagg acctcgctcg ataacagatg cgtcctgcac
      181 gcggtacccg ttagacggcg acgatccgca cgcgccgtcg gatagcgtac tctagcacga
      241 tcgatgacaa ggcgggcatc aaaaattcac acggagattc agtgatccga cgtgcaaggt
      301 ggtgcgatag gttccattga cggtcggatt cacgttgccg gcaacggtcc tggattcata
      361 caaaatggcc tggtaagcga gatcgatgcc gagtcccttg gggctgagtt tgctcgcccc
      421 tccgcagggt atcaccccaa gaaaccgtcc gttgtcccga cacatgaacc cgacgccggt
      481 agacaccgca tggttgtcgc cgtcggggat ggcggggttg aaggtttgat cggggatcgc
      541 cttttgtgaa tgccagtacc cggcgcggag ggcgatatcc caatcgggaa gcgatgccgg
      601 gttgagccac ttgtgttccg tgccgatcat gacggtgtaa ccgctgatcc aattggccgg
      661 agtggcgacc gtgatgccgt ttgaaagatg tgaatccagg ttacgaactg atttccaccc
      721 cgtatagtcc acatccagtt ccagtttcca ttcgcgttct ttattgcgga caggccagag
      781 ggcgatgcct cccgtcagga cctgtggcac tacgaaggtc gtgcgtgcgt ccgcgatatg
      841 tgtgccgccg gccagcaatt ggccgtcgag gtgcagggtg gcctggctgc gatagataag
      901 gccgatgttg acgaggggtt tgccatcttc attgcgcagc ggggtataca gcaggctggc
      961 attccagccg gcagcggtgt cgcgtccgtt taactccatg ccggtgccgg ctggaatgcc
     1021 gagcccgccg ggccagcgaa attgccgttc gacctgtcct tctccgaagg ctcctgaaaa
     1081 cgtatacaca tccgctccca gtccgatggc gaggtccgga agcggtctga aagcgatgga
     1141 cggacgaatg tcgatcaact caaaagcggc cttagtggtg gccgtcgaaa aggggctgct
     1201 gtccggccag cgagtcaagg tgccgaaggg gctgaagacg gccagcccga ccgcagtgct
     1261 gtcggaagag ccgatgccca agtccttgag attggccgta atatacagat tggacggcgg
     1321 cggaaccgcc acgctgccgc ggaggtctcc attggcctgc gttcctgctc cgttctgaaa
     1381 ggaaaaacca ccggcgatca gcgtggtgcc ggctgaaagc tgcacacccc gcaactgtgt
     1441 catgccggcg gggttgtaat agccggcgga ggcatcgtcg gcctgagctg tgaacgcaga
     1501 ggcttgaccg gtcgccgagg cgctatggtc atagatgcgg aaacttccgg cgagaaccga
     1561 gcctggagcg atggtcgacc aggcggccag cagcgcgccg gtgaggagga gcaggcgtgc
     1621 ggtggtgcgg gcgttccgac ctggagcgac cgtttcccgg cttgctgacg acgggtcggt
     1681 ctcggaggaa gcaggggcag tgcagaagcg tatcgtactc accggtcatt cctcatatag
     1741 ttctaggtac aagatcgtag ccatatggtc ttgtgcggct catctaagag aaggacgggc
     1801 attttgtcaa gcaggggcaa gccagaccga ttgctggaag ccgcggcgaa tgtgttttta
     1861 tgcgcgggat cgcgtatgct cggcggcatg aggattcggt ggtgagattc gtaaggcggt
     1921 ggcttctccg cgcgcttgtg gcggtgacag gcattctcct tctcgtcctc gggagcgccc
     1981 tggcttatgg actgtacctt tctgccagcc ttgcgttgcc ggcaggagat gaacatcctc
     2041 cacgcctcat ctatggcgcg ccctttctat taaagcccga cttggatgtg gtctcctcgc
     2101 atttgatcga gcgcctgagc cgactgggtt atcaggcggt cgacacgccc gtgcgcgcac
     2161 cgggagagtt tcgcgtgagt cccggccaga ttgatatcta tctgcacgaa ttttccgact
     2221 tgcatctgcg gccgatgccg gcccgtttgc tgttggagca gggacgtgtg acgcgggtcc
     2281 tgtcgatcca gaccggcgac gaactctttc ctgcctatct ggagccgcag ttgatcagcg
     2341 gactgcgcgg ggcatcgcgg caggtacggg aatgggtgtc gtacgacgac ctctctgcgc
     2401 gattcctcga tatcctgctc gccatcgaag accggcggtt cttcagccat cccggcatcg
     2461 atccgatcgc cgtgggacgg gcagtgtgga cgaatgtcac caagggaacg gttattcaag
     2521 gcggcagcac gatcacgcag caattggcca agaacctgtt ttactccccg cagcggacgt
     2581 tcgggcgaaa gctgaaggaa tccatcgccg ccttggtgtt ggagacgaaa taccccaaaa
     2641 aagccattct cgaaagttat gccaacgaga tctatctcgg gcaggtcggt tcggtgtcga
     2701 tctacggcgt cggcgaagca gcccaccggt atttcggcaa acgggtggat accctcagtc
     2761 tggaagaggc ggcgttgctg gtaggcatga ttaaaggacc gaatacctat tcgcccttga
     2821 gaaatgccgc cttggccaag caacgtcgcg atgtggtgct gggccggctg catgaacagg
     2881 gcctgatcaa tgacgaggcc tgggctcagg cgatcaagac gccggtgcaa gtcatgccgc
     2941 cgcaggacac gctggccgat gcgccgtttt tcgtcgacta tctcttgcgg caggtggagg
     3001 aggcgaccgg ctcgccgttg ccggacggtg tgagggccta tacgaccctc gatcctgtgc
     3061 tccagcgact ggccacccag actcttgaaa gcgaactcat caagctggag acgacttatc
     3121 ccgcattgaa aggccatccc acccctttgc agggcgcgtt ggttgcgatc gatcccaaaa
     3181 ccggcggcat cctggcgatg gtgggcagtc gcgattatcg tacgagccag ttcaatcggg
     3241 cggtgcaggc gaagcgccag ccaggctcgc tcttcaagcc gctggtgtat ctcgccgcca
     3301 tggaggcgcg tcgtgaactg aatggcgggc aaccggtaac ggcggctacc tcgatcgtcg
     3361 atgagccggt gacgtttgaa tcggccacgg gccggtggac gcctcagaat tacgaccata
     3421 aatttcacgg gacggtgtcc ctgcgaacgg cgttggaaca gtcactcaat attccggcgg
     3481 tcaagatcgc gcagctcgtc gggacgaaac gcattctttc cctggcagag aaactcggta
     3541 tccggagtcc gctggccgat aatctttcca tcgctctcgg gacctccggc gtgtctctcc
     3601 tggagatgac ttcggcattc ggcgtcctgg cccatgacgg ggtcagcatg actcccacgg
     3661 cgctgcaggc ggtaatgacc tcggagggag atccggcctg gcatcacacg ccggctaggc
     3721 atcaggcggt gtcacctcag gcggcgtatg tcatgacgtc gttgctcaag ggcgtggtgg
     3781 aacgcggaac tgcggcgaag gccaaagcga tgggcctgcg cgggattgtg gccggcaaga
     3841 caggaacaac ggacggctat cgcgacgcct ggtttatcgg ctatacgccg gatgtggtcg
     3901 taggggcatg ggtgggattc gatgatgagg acgcgttgca cctgaccggc gcacaggccg
     3961 cattaccgat gtgggtggag tttgtgcgcc agattctgcc ggctgcaccg cgtgactttg
     4021 tcatgccgcc agccgtgatc acgcgtgaca tcgacccgca gtcctcgcag ttggccacct
     4081 cgaaatgtcc gcaacgttcg gcggagctgt ttatcgaagg cacggagccc tcggtgtatt
     4141 gcgaagtgca cggaagcggc atgtgggaac gggtgaagcg gagttttggt tttttctagg
     4201 tgtcatcgaa aagctggtgt aggctcggct catgttgaat tggttctccg gttcatccgc
     4261 cactgcccgc gacaaggagc tcccgatgat gaagaaaccc ggctttgtgg aaagcgacaa
     4321 tatcaccctg ctggccaaag gcgtgttgct caggggagaa atccatgttg aaggcacggt
     4381 gcgcatcgac ggccggctgg agggagattt gaagaccagc gggacggtgg tgatcggcga
     4441 agacggagtc gtgcagggca ccatcacggc cggcaccatt atcagcagcg gaaaaatcaa
     4501 agccacggtc cgggccacgg aacggattca attgttgaag accgggctcc tcgtcggcga
     4561 ggtgcacgcg ccggcattct cgatggaaga cggagcgaaa tttcagggcc tttcggatat
     4621 gggcgtcacg tcgtggggcg atgacgcgca gaagcttccc ggtaacgtac gggagattgc
     4681 ctcccagcgt cccaaagcgg tggccatggc cggggagaac gcctgaacgg cgtctctccc
     4741 gacctcccgg ctatctgatt cggcttcttc acgcttaccc ctccgtctca ttgctcagcg
     4801 ataagtcttc cgtacaggca cgaggatcgc aaaaccagcg cgtgacatca cgggcgggtt
     4861 ctgctatcaa cggcgcaggt acaaacatct ataccccctc atgaatcgac gacagatttt
     4921 tgcagcctgc tttttcggcg tcctgctggc gctgctgtat cagatggccc tcatgtttcg
     4981 cccgtttgtc ttcccggtcc tgtgggcggt cattctggcc cacctctgct tcccactcca
     5041 tatccgattg acggcctggc tgggcgggaa ggaatcgcat tccgccgtac tcctcacgct
     5101 ggccgcgctg gccttggttg tggtgccgct ggtcgtcctc agtgtgatgt tagtcaaaga
     5161 ggccgggtcc gccgaacgtg cggtacggga atggatcgcc tcgggaggcg ttcaacagct
     5221 cccggatcgc ctctcgggac ttcccggtgg aagcgtggca cgggagtggc tcgagcgatt
     5281 cacggggcag gaaagtgacc tggagcaatt tgtgttgtcg agtgccaaga cgttcagcaa
     5341 atttatcgtc ggggaattga gtgacctcgt gcgcgacgtg ttcatgctag tggccgattt
     5401 tctggtcatg gtgtttacac tgtttttttt cttcaaagac ggccggcatt ggctcgcatc
     5461 actgtacggg ctgattccgc tcgaggcttc ccataaggac aagattctcg tccgtctgga
     5521 ccaaaccatt cgtgccgtgg tcaaggggat tgtgctgacg gctgtcgcgc agggactcct
     5581 tgccggggcg gcctatgccg tgttgggcgt gccttttccg atggtcttga tggcactgac
     5641 cattctcctg gctcccttgc cgttcggcgg cacggcgctc atctgggggc cggtggccgg
     5701 ctacctgctg tggtccggga cggtgggcaa gggcttagtg atgctggcct ggggtattgg
     5761 cgtggtgtcg accatcgacc agattctgaa gccgctgttc atcggccagg gggcgcagat
     5821 tcccgtcttg ctgctcacgt tcagcgtgct cggtggattg gcagcctacg gcattctggg
     5881 cctgtttctc gggccgattc tcgtcggatt gttcctcacc gcgttgcaga tttaccgcga
     5941 cgaataccaa ctgcagcccg cctcgccgat cgtcaggtcc tgaccctcgt tgggcgcctc
     6001 ttccctcagt cgagaggaat tgtaatggtc atccctccga tcacatcatt gagtgcgaag
     6061 tcccgtttag ggctcagagg atgactattg tgacacttga tgcaggcggc ggaaatcgcg
     6121 cgatccgcat agaccgcttg gaaaaattgt ttgcggccgc tggtgacaat gccggtgaac
     6181 gggcgatcgg gtgtctgcgc gaaactctcc agcgcctgcc gctccaaatc cgtcgccggc
     6241 gcattgcgtt ggtaaatcgg ccagagactg atcagccggt aacggatgcc gcgtccggat
     6301 tcagcgacca gcttgcccga gtgttgcagg aattgcgcgg gtaacgggag ggcgttttca
     6361 tgctcccaat gttccatcgc ggagacgatg cctttggcct gcatgcggtt gacgacctgg
     6421 ttggtataaa tcgtccggtc ggcatcgagg acggcatgga cgaagtccgc gaccttttcc
     6481 ggggagatgc ctgccggtgg ggtgaggtcc ttcgcctgca aggaataggt ggagacacaa
     6541 gcgagaaaga gcgccatcca tatggtccat gatcgtacac ctgtcaggtt gaaatgcatg
     6601 gctcctcctt tcgtaccgcc gccttctctg ccggcagcgt aatgacgcag gtggtgcctt
     6661 ggccttccgg gggactctcc atccggattt ctccgccgaa tttcttgatc aaccgctgcg
     6721 cgacggtgag cccgaggccc gatccttcgc cttgcccttt ggtcgtataa aacggatcaa
     6781 agactttccc aagatgctgt ttgggaatgc cgggtccgtt gtcacgaatc ttgatggtaa
     6841 tcacatcgtc ctgctcttcc gtggtcagcc acaaattgcc ttgcccgttc atcgcttgaa
     6901 tggcattggt gagaacattg atgaaagcga ggcggagctg gtcgggaagt gccgtcacgg
     6961 gggatacagg gacataatgt ttttggacgt tgagccccag gcattcgtgt gaggtctgca
     7021 cgatagccag tgcctgttcc agttgagccg tcacatcgac cggcatccgt tggctcttcg
     7081 attcgcgagt cgccacaccg gtgaagtcgc ggatgatggc ggccatcttc cggccgtgac
     7141 ccacgatgtc ttgcgcgtag cctttggcgc gggccagatc cccttcttcc tgaatggctt
     7201 cgccaaggcc gagaatgccg aacaagggat tgttgagttc gtggccgatg ccggcggtca
     7261 gcacgtccaa gctgcctgat ttttcagcct gaatcagctg atcctgcaag cggctctcat
     7321 cggtcgtgtc ccggaggaca agcccgaatc gttttcccgc gccgctgcgg ctttccagcg
     7381 ggaaccactc atactgatac agatgcggac cgatgcggag ctcgcgccgg gatgaagcag
     7441 gggcgggacc gatcgtctga ttgagcggat cgcgcgccgg cttcagcaag ggaggcgcgt
     7501 tgtggcggac tggcgccagg gacatgcctt cctcgaccgc ccggaagtcc cgctgcagcg
     7561 cctggcgatg ctcctcgtcg acgggcaaga ggtcgaacag cggtcgcgat gaccagctgt
     7621 ccgcctgcac cgcctcacgc gacgccttat tcatatattg gacgagccct tgttgatcca
     7681 ccatgatgat aggcgttggc acggcatcga gcacctgatc agtggctttt tggaggtact
     7741 gtacttcctg cgttttttct tcgacctggg tgctcaagcc ggcaaaggac gcttccaatt
     7801 gtttgtgcat gcggttgaat tcgaccgcca ggtcttccag ttcatcgccc gtgcgaatct
     7861 ggatgggctc ctgcaattct ccgcggccga tgaggcgggc cgcctcctgt aagcgacgga
     7921 ccggcgtcac gatgcggcta gcggcaaaat agcccagcgt ggcgagcagg aagagggcaa
     7981 ccgaggccag gatcagcatc cacagcatga gatgtcggat tggtgagaac agttcgtcgg
     8041 aggactgcca cacgaaggtg tgccacgccc caacctccag ggcgccgttc gtggcgcgac
     8101 tggtttcagg gagcaacgcg aacccgataa tggccgtgcc ttgtccgcca tgcccatcgc
     8161 tgtcggctgt gacccacccc ggttccctga cggtcacttg cggaatgagc gccgggtctg
     8221 atagccgaac gccggtgggc agaatgggac agctcaacac gattcctcga ctgtcgatga
     8281 gcatgacgtg gccggtcttg ccgaaccgga tgggatgtgt cgagggagag aaaaattcct
     8341 tggcgtcgat gacgcgatgg aggacaccca ccacttcata tttcaggctg tccatgatcg
     8401 gcagtgaaac actgaagaca taggtcttgg cctgttcatc gaaatgcaga tcttcgatgt
     8461 acagctgccc cactcccttg ttgtaggttc ctttccacca cggactgtcc gcattggaaa
     8521 aaccggggaa ggtggtgagt gcagcgaaca aattgccctg cacgtccgtg atgaacagct
     8581 ttttcgtggc ggcgcgtacg acttgaggaa gcagcaggtc ggactcattt tgagcccccg
     8641 cgtaaaagct ccgcagcaga acggcggtgc gattttcggt aatggccttg acggcaatgg
     8701 gatcgcgcgc ggcccagcgg gttcgttgtt gggtgatcgt cgtggcggac agagttttgt
     8761 cggcggtctg cagcagatct cggtggtgct ccagttgtcg gatgatctcc gggtgtgtgg
     8821 cgatgcggga cgttctggcg acttcctcgg ccacgagcaa gtccagtttg cgggcggctt
     8881 ccgtggctaa ggcctggaag ctttcgccac tgacgacttg gatttctttg gagccttgca
     8941 ggaaggccat gctcaggccg agtagcaggg gaaccacgcc gaccagcagc atcgagacga
     9001 tgagcttacg tttcaggccc catcccagcg gattggagaa ggggcgccag gtgatcgtca
     9061 taggggcgcg catgatgtag tcggttgcac ctgtggaaat tcgatccgga aggtcgtgcc
     9121 gacaccgacg tcgctttcga cacaaatgtg tccttccatt ttcgaaatca ccgacatcac
     9181 attgtacagt cccagccccg tgcctttccc cgggggtttc gtggtgtaca ggggctcaaa
     9241 gattttcgag aacgcctcct tcggaatacc gcaacccgta tcgctcaccg acgcgctgac
     9301 ctgtccgtcc gggccaacgt tcgtgtgcag ggtcaacgta ccgcggtcct gaatagcctg
     9361 aacggcgttc gtaattaaat tgacgaagac atgaagcaac tcgtcagggt tccctaagac
     9421 cgaggcatcc tcagcatacc gtttcacgac ggtgacatcc tgcagggtca cggcgtaacg
     9481 ggcgattctc agcgcctcat ctaatttggt gttgagattg accatgatgt cttcccccgt
     9541 cccgttgcgt cgtgcatacc gggtgaggtc gcggcaaata gcgctggtcc gtttcacggc
     9601 ttcggtgatg tcgcgcgcct gttcgtgaac gacggtcaaa tcttgctcgt ccgtgagatt
     9661 ttccgcaagg ccgaggatga gctgaagcgg gttattgatg tcatgcgcga tgcccgccgc
     9721 aaaggaccct aatccagcca gtttatcggc ctgaaacagt tcggcctcca gtttcgactg
     9781 gtgctggacg agtttccata acagccgcgt cgtggctccg ctcaagactt cggcgccggg
     9841 acgtttcatg gcttggatga agggggccat ggatggatca cccgtcacat ccatgatgag
     9901 gttgacgcca tggttgcgaa tgaggtcgta cacctgttcc tcaactggca cattcaaatc
     9961 tttcgctcgt tgcaggccgg gcgcggcggg gtccttgtcc gcgatgccca cgatgtctac
    10021 gtcgggaatc tgatgcagga gctcgagcag tgcggtgccg cctcgtcctg cgccgatgat
    10081 ggctatcttt gtgctggtca gtgtcatgcc tgactccggg tggagggacg aaaacccctg
    10141 tggtacaggg cgtcgccgtc ggggaggtga tcaaactctc cccgacggcg agtgctgaag
    10201 acggatacgg caggggccct tgctagagct gggcgagttc ccgttgctcc atagccctcg
    10261 ccacggcggt gcgaagtttt tccccttcca ccggtttcac caggtaatcg acgacgccac
    10321 tgcgcaggaa ggaggtggcc atgtcagtgt cgggaaaacc ggtcagcacg ataatcggga
    10381 cgcgcggcca ttcctgttgg aagtaggcga tggcttcaac cccgttgatc ttcggcatgc
    10441 ggatgtcaca gatgagcacg tccagcagca gccggttttc tccggtgttg atggcctcta
    10501 tggctttttc cccgtttccg cttcgatgac ctcgtagccg gccttctgca gcgtcatgcg
    10561 gacgactttg cgaatgtccg gttcgtcatc cacgaccagc acccggccat tgcaggcggt
    10621 ttcactcatg aaaaagcccg atttgaattc tcccatgatc ccctccttgg tggtgtgacg
    10681 aggtgtaatg tcgtgaccca tttgttcata ggctacagca acagatgtgc ctcgaaggga
    10741 gagaggaaga atcactgaat ttcaggcagt tagccgctat tctccggaga gagctgcagg
    10801 gctggtggtt gggatccacc agccctggtg aaatgcacca ccgggatgcc ctcgctagag
    10861 gagcccatta cggtgataga aacggccggt ggtgcaagtg aagaaggcgg gtcgtattcg
    10921 ggcgggcgcc tgctaaccga ttatttggcg ggcgggttcc cgaagaaatg caggtctttc
    10981 tcccccctga gatgttgcgg cgtcacgaca tattccccgc cgatagccgg cccccagatt
    11041 ttgtcgaccg tagccttgtc tcccccggta aaaatgaacc ccattcctcc ggcgacggct
    11101 ccgagtacgg cgacggccat cttgattccg ccgtagggta aggtcagaaa ataacttccc
    11161 aggccttgaa tggcttccga tccggaaccg gccgccgaag ccgttctcac cgagagcgtg
    11221 gcttggcatc cgaggaccag gaccaacacg aacaaggtcg tttgtacgag cgggcgtggc
    11281 tgtcgagact tcgtctgaga catgagtact cctttctgcg tatgggcata acctaggcta
    11341 cgtatcgtcg cgtagtgtag cgggccagct gacaaattgc catctgtgga gcagctggac
    11401 gcatctcata attgactcag cgcctcacat tgccttccgc ttcccggatg cgttattctg
    11461 ctcacagatg attaccgccg aagccgtaac gacctatatc cgtcaaatgt ttcccgatgc
    11521 cgctgtcacg gtgattgata agaccggtac gcaggaccat ttgattgtac gcgtgatctc
    11581 tacgggattt gccgataaga atctcctgga ccggcatcgc atggtctatc aggcattagc
    11641 gggtccgatg aaagatgggc gcatccatgc gctggagatt acggctacga caccggatga
    11701 agtgaaaaaa taagggggac cagatggctg acccgattga agaagaaatt cagcgagaaa
    11761 ttcaggccaa taaaatcttg atctacggca agggcaccaa aaccatgccc atgtgcgggt
    11821 ttacccggga gacgatgcat ttttttgaaa agtacgggta tccctatgaa ctcatcgacg
    11881 tcttatctca gcccgccaaa cgcgaggcct tgaccaagtt gacgaattgg ccgaccttgc
    11941 cgaaggtctt catcgacgga cagttttatg gcgacacgga tatcctggac cccatggaag
    12001 cgaaggggga ggtggtgccg ctgttgaaaa aagccttcgg cgccgaatcg taaggctttt
    12061 cgggcctgta agcctggtac atgattcctt ccggcattcc gccggtgctc tttcgaaaga
    12121 gggcgtcttg tctgcgaccg gacgaggcgc ct
//
LOCUS       DAOQEW010000113        12141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1536_length_12141_cov_9.140493, whole genome shotgun sequence.
ACCESSION   DAOQEW010000113 DAOQEW010000000
VERSION     DAOQEW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1536_length_12141_cov_9.140493"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLS35_12440"
     CDS             <1..1086
                     /locus_tag="PLS35_12440"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19521.1"
                     /translation="IDTLVSELTHEEEWRRMRATAACLAGGPRAVQALTHALQSGDVP
                     LRVEAAAMLSRIKDPAAGVALVTLIRDPEATVRQAAFAALEQMAGNLDDETAAGLVRN
                     LHESPDEEVRHRVRQLLGVIPNAITPLCEMLRHPDAEAQMTAATILEHLLDPRSADGL
                     IDAMASPAVREIVVRTLKKLSAVRERIDATFNALRDVEGASEREEARMATVMDLLGIG
                     RPSVEILIEYLEDDDWLIREAAADLLGKIGDVRAVEPLMKRLQKDKDTGVKEYAVKSL
                     GLIGDPRPAQLYIEAIPIRPLRVMAIEALAKIKDVEVLRPYNELFNRLRSDRDGIVSY
                     SAGLIADKLAALEGEQPVGQEESDDHE"
     gene            1079..1840
                     /locus_tag="PLS35_12445"
     CDS             1079..1840
                     /locus_tag="PLS35_12445"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19522.1"
                     /translation="MSDAERIAQLIAALRDDNEALRDHAMASLGQMGIEAVPQLIGLM
                     ADEDVVIREAAATAVVRIGPIAFDQLLEALRDDEWAIREQAANVLGRFRDPRAIDPLM
                     VALKDKDGAVRTAAVWALERIGDSRATPGLIEALGDGTVREDVARVLKKIGDTRAVDA
                     LIGGLLGPNWMVRRHAAEALGKIGDPRSADALIQSLQDEDWLVRRNAAESLARLGAKQ
                     AIEPLLPLLEDENTMVQETVEGVLASLGWKQATSS"
     gene            1861..2571
                     /locus_tag="PLS35_12450"
     CDS             1861..2571
                     /locus_tag="PLS35_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI19523.1"
                     /translation="MAEEAPVKLIQIGPKGGPKKDGFNLVTERVVAVNPEARQLEVEL
                     LAYDGKTVVLDVGEEALEDFLKIKPGDGATIRVVEEGGKRVAKSFRIRAKDPNAAKAD
                     AMLIDLKDSHWLNRKYAAEVLGDLKDPRAVVPLVEALTDEVGDVRQRAYDSLIKIGGS
                     AVPSLVPLLASEEDDVRQSATEIIRKIGKPAVEPLATALAEADDRLKTKIMKVLDRMG
                     YKPKAKEGAQPEPAKLLS"
     gene            complement(2593..3912)
                     /locus_tag="PLS35_12455"
     CDS             complement(2593..3912)
                     /locus_tag="PLS35_12455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNI19524.1"
                     /translation="MHISVIGTGYVGLVTGACFAEFGVNVTCMDTDARRIAKLEKGEV
                     PFFEPGITELVAKGIKEDRLHFTTDVAKAVDKALVIFIAVGTPPKSDGSADLSYVEEV
                     GRGIAKNMTGYKVIVTKSTVPVGTGEKLREVIKANQTGRFRFDIVSNPEFLREGSAIE
                     DFMRPNRVVIGADSEQAVAIMKDLYRPLYLLETPIVVTDIPTAEMIKYASNAFLAVKI
                     SFINEIATVCEKVGADVQLVSKGMGLDNRIGNKFLHAGPGFGGSCFPKDLAALVQTGE
                     RVGYPFQIAGAAAKVNYEQHLRMVQKVKDACGGVKGKTLGVLGLSFKPNTNDMREAPS
                     LTILSELMKEGAKIRAYDPASMEESMKLLPGMVPCQDTYEVAEGADGLIIMTEWNQFR
                     NLDFERLKKAMREPLLLDLRNTYESDRVVPFGFRHVSVGRPTKNPAA"
     gene            4110..4520
                     /locus_tag="PLS35_12460"
     CDS             4110..4520
                     /locus_tag="PLS35_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI19525.1"
                     /translation="MVVAGGPIQRPLAMMMRPITKTVRPDDSLLVVAQQLRDARVGAM
                     LVADHGDYVGIVSEADLVRKAMASGAASDQVTVRSVMSAPLMTIDIAQSAHEASDVMA
                     ERGIRHLVITDEGRVVGMISVRDLLRYFKNWGTL"
     gene            4584..6158
                     /locus_tag="PLS35_12465"
     CDS             4584..6158
                     /locus_tag="PLS35_12465"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNI19526.1"
                     /translation="MMAQRPAVVPIPRLFLLFTALVTGAIVMALEILGSRLLAPVFGN
                     SLFVWGALIGIILAAMSSGYAFGGWASDRYRVAAVLAWLLLGSGAWTLLMSWMGQGAI
                     FQVAKLVEDPRWGPSLAACVLLAPPAFGLSGVMPALLRLAVVDMGYLGRHTGSMIALS
                     TVGSLAGTWGTAFFLLSWLGTQTLVATLGVIQLLLGLLWLQQGSGRVTKLTGVVMTGL
                     LISGWQLFGQAPPVAGLVYQEDSPYQQVRVRDDDLFRYLVLDRTWHAVMWRSDPTALF
                     LPYSQLMVAAVALAPEPKRGLILGHGGGSLAKWLAQVWPELELDVVEFDPVVVRMAEQ
                     YFDYRPPANHHVSVKDARVFLRDTKATYDVIWVDAFARHLIPFHLTTVEFFSELRGRL
                     NPNGVLALNLASSGEGGDLQRANAVVQTLKSSFPSIESFGVKGPWRAHQTTAENLIFF
                     GGGPIATMSYDEIVKRIYMQVEARRLPLEAIALLAARRTTPWPPGLTLTDDYTPYDLL
                     IGSHAVEMSPEGNSSP"
     gene            6433..6912
                     /locus_tag="PLS35_12470"
     CDS             6433..6912
                     /locus_tag="PLS35_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19527.1"
                     /translation="MGKTMVAVFFGLVMLMAGASGAYALQAGGVEIGDLDTGSVVGQP
                     FKELIVDAELYAVEIGNLKAWYPPVTVIDFKVRPGRPVVLKVTNNSSSEHGFQMTDPV
                     NAASPFVMKAEVVLKPGETKYIGVPTSDMFNATQGNTLTYRCHLHPAHVGGKLVMIK"
     gene            complement(7007..7342)
                     /locus_tag="PLS35_12475"
     CDS             complement(7007..7342)
                     /locus_tag="PLS35_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19528.1"
                     /translation="MNCFTRLACIAILALAPLPSYAMTEVGDIGTIIESFITKQFPDA
                     ASHFWVVNDTQWDGDEMIVDMNTVVTDRREAPPTESRFLLLIVEGRLQGIQNIPLDAA
                     AECQKEQEV"
     gene            complement(7555..7902)
                     /locus_tag="PLS35_12480"
     CDS             complement(7555..7902)
                     /locus_tag="PLS35_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNI19529.1"
                     /translation="MALLITDECISCGACLPECPNEAIFETRSDAEGKGNHVGDGQGV
                     GDNIYVITHDRCTECVGHFDEPQCAAVCPVDNCCIADPAYPETTDVLLEKAKSLNPDK
                     EIDPAKVWSGVRN"
     gene            8150..8647
                     /locus_tag="PLS35_12485"
     CDS             8150..8647
                     /locus_tag="PLS35_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNI19530.1"
                     /translation="MVTRASGSESEQRRKKIVTFVLLVILLISVSLFLGGPKESELII
                     VEFPNGKTMETEVASTPEKLLFGLAFREGLPADTGMLYIFESTGLHRVATRQFRIPVD
                     MLWIDESHHVVHILEQVQPCLQDPCPFYGPPPEPVRYLIEAEAGYAKRAGITIGMELK
                     FTLRM"
     gene            8672..8989
                     /locus_tag="PLS35_12490"
     CDS             8672..8989
                     /locus_tag="PLS35_12490"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNI19531.1"
                     /translation="MEGFQCVAKVEDIPPGQVKVVKVNERAIAVFNIEGHFHAIYNSC
                     PHEGGPLNKGRVKGHVVSCPWHDLAFDVRNGQGTDGGGYCVGSYDVRVEDGQIFVGGR
                     RKV"
     gene            9025..9357
                     /locus_tag="PLS35_12495"
     CDS             9025..9357
                     /locus_tag="PLS35_12495"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNI19532.1"
                     /translation="MSPLSKAVPTGTEQEPGAKVVKARVGLPVHIRLWEDRTRGELWV
                     PIYDAAVMPLVEDDFLRTASNNAVDQGQRSFEFKPSVAGTHRVVFEKRLGWKFTAEDR
                     QIFYVTAS"
     gene            9382..10221
                     /gene="dat"
                     /locus_tag="PLS35_12500"
     CDS             9382..10221
                     /gene="dat"
                     /locus_tag="PLS35_12500"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HNI19533.1"
                     /translation="MPDIACVNGCFGPVAEAVVSIEDRGFQFGDGVYEVIRTYRGKPF
                     ALDEHLARFERSAQALHLSLGLTKDQWMALVQEGLRLSQFPETKIYLQVTRGQAPRDH
                     LFPASTVPTTVLTFRELHPLDASIRKAGVGAVTVDDIRWGRCDIKSVNLLANVLARQR
                     AKEAGVFEAILVRDGLVTEGSVSNVMVVRNGVVETAPEGHRILSGVTRAMVLELARKE
                     GLPVVETFVGREDLLAASEVFLTGTTVEVLPVVRIDGQPIGAGVPGPLSQRLSRRWEA
                     LVG"
     gene            10311..10832
                     /gene="rimP"
                     /locus_tag="PLS35_12505"
     CDS             10311..10832
                     /gene="rimP"
                     /locus_tag="PLS35_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNI19534.1"
                     /translation="MSDAGAVSAGKPLVKRAEALNLRVQEVAAPILQSHGLELVEAVC
                     VGQGPRTVIRVFIDKPGGISLTDCEQAHRSLSPALDVIDPFPHAYTLEISSPGLDRPL
                     RTAHDYRRLIGEPVTLKLREPIQSQWRLVGTVLEVEDGAVTLAIQQKKTTETIRVEFD
                     HIAVARRKVEFSK"
     gene            10874..12013
                     /gene="nusA"
                     /locus_tag="PLS35_12510"
     CDS             10874..12013
                     /gene="nusA"
                     /locus_tag="PLS35_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNI19535.1"
                     /translation="MNRELIAVIDEIGRQKGIDKARVIGAIESALQTAAKKRFGQAEN
                     IQVEIDSKTGEISVVSKKIIVDTVANPKAEISLKEAREYDEGAEVGDEIGSLIEMDEL
                     GRIAAQTAKQVIFQKVREAEWEAVQKEYSTRQGDLVNGIILGMERRNFLVDLGKTEAI
                     LPIQEQIPRETYRRGDRVKALLLEVRRTPKDVQVILSRSHPQFVAKMFELEVPEVGEK
                     IVEIKSIVREPGDRTKIAVSSRDKAVDPVGACVGIKGSRVQAVVRELRGEKIDIITWT
                     QDPRVFIAEALNPATIEKVGIDEEKKSALVVVADSQLSLAIGKNGQNVRLAARLTGWK
                     IDIISATEYEKEKAERDRDIKAALAEETEAQRQQDEARAAAQQAE"
BASE COUNT         2475 a         3141 c         3821 g         2704 t
ORIGIN      
        1 atcgatacct tagtttccga gttgacgcat gaggaggagt ggcggcgtat gcgagccacc
       61 gcggcgtgcc tagccggtgg tccacgggcc gtccaggcct tgacgcacgc gttgcagtcg
      121 ggcgacgtcc ctcttcgtgt tgaagcggcc gcgatgttgt cccgcatcaa agatcccgct
      181 gcgggtgttg cgctggtcac cttgattcgg gatcctgaag cgacggtacg gcaggcggca
      241 tttgccgcgc ttgaacagat ggccggtaat ctggacgacg agactgcggc gggcttagtg
      301 cggaaccttc acgagtcacc ggatgaggaa gtccgtcacc gtgttcggca gctcttgggc
      361 gtgattccca atgcgatcac gccgttgtgt gagatgttgc gccatccgga tgcggaggct
      421 cagatgacgg cggcgacgat tctcgagcat ctgctcgatc cgcgttcggc cgacggattg
      481 atcgatgcca tggcttcgcc ggcggtccgc gaaatcgtcg tgcggacgtt gaagaagctg
      541 agcgcggtcc gggaacggat cgatgcgacg tttaatgcgt tgcgtgacgt cgaaggcgcg
      601 agcgaacgcg aggaagcccg catggcgacg gtgatggatc ttctggggat cggccggccg
      661 tcggttgaga ttctgatcga atatcttgag gacgacgatt ggctcatccg tgaagctgct
      721 gccgatttgc tcggcaaaat cggcgatgtg cgtgcggtcg aaccgttgat gaaacggctc
      781 cagaaggata aggataccgg cgtgaaggag tatgccgtga agtcgttggg gctgattggc
      841 gacccgcggc cggcccaatt gtatatcgaa gcgatcccga tccggccctt gcgcgtgatg
      901 gccatcgagg cgctggccaa aatcaaagat gtcgaggtct tgcgtccgta taacgagctg
      961 ttcaaccggt tgcgaagcga tcgggacggt atcgtatcgt acagcgccgg cctcattgcg
     1021 gataaacttg cggcactcga gggcgaacag ccggtcgggc aggaggaatc ggacgatcat
     1081 gagtgatgcc gagcgaattg cacaattgat agcggcgctg cgcgacgata atgaggcgtt
     1141 gcgcgaccat gcgatggcga gcctcggaca gatgggaatt gaagccgttc cccaattgat
     1201 cggcctgatg gccgacgagg atgtggtcat tcgcgaggcg gccgcgacgg cggtggtgcg
     1261 tatagggccc attgcgttcg atcagctgct ggaagcctta cgagacgatg aatgggcgat
     1321 ccgcgagcag gccgcaaatg tactcggccg gtttcgcgat ccccgcgcca ttgatcccct
     1381 catggtggcg ctcaaagaca aagacggggc tgtcaggact gcggcggtct gggcgttgga
     1441 gcggatcggc gactcgcggg ccacgcccgg gttgattgaa gcactgggcg atggcacggt
     1501 gcgcgaggat gtcgctcggg tgttgaagaa aatcggcgat actcgagcgg ttgatgcgtt
     1561 gatcgggggt ctcttgggcc ccaattggat ggttcgacgc catgcggcgg aagcgctcgg
     1621 aaaaatcggt gatccgcgca gcgccgacgc gttgattcaa tcgctgcagg atgaggactg
     1681 gctcgtccgc cggaacgcgg ctgaatcctt ggcgcgcctc ggggccaagc aggccatcga
     1741 gccgctgctt cctctcctgg aagatgaaaa tacgatggtg caggagaccg tcgaaggtgt
     1801 actcgcgagt ttgggatgga aacaggcgac ctcgtcataa cccgataaag gaactctact
     1861 atggcagaag aagcaccggt aaaactcatt cagatcggcc cgaagggcgg acccaagaag
     1921 gacggcttca acctggtgac ggagcgcgtc gtggccgtca atccggaagc gaggcagctg
     1981 gaagttgagc tgttggccta tgacggaaag accgtcgtgc ttgatgtcgg agaagaagcc
     2041 cttgaggatt tcctcaagat caagccgggt gacggcgcca cgattcgtgt tgtcgaagaa
     2101 ggcggcaagc gagtggcgaa gagcttccgg attcgcgcga aagatccgaa tgccgccaaa
     2161 gccgacgcga tgctgatcga cttgaaagat tcgcactggc tcaatcgtaa atatgcagcg
     2221 gaagtcctgg gtgatttgaa agatccgcgg gcggtggtgc ctctggttga ggcgctcacc
     2281 gatgaggtgg gcgatgtgcg gcagcgggcc tatgattcgt tgattaagat cggcgggtcg
     2341 gccgttccct cgcttgtccc gttgctggcc tccgaagaag acgatgtgcg tcagtcggcg
     2401 acggaaatca tcaggaagat cggcaagccg gccgtcgaac ccctcgccac ggcgctggcg
     2461 gaggctgacg accggttgaa gaccaagatc atgaaggtat tggaccggat gggttataag
     2521 cccaaggcga aagaaggggc tcagcctgaa ccggcgaagt tgctcagtta gcgagacccg
     2581 tccggccgcc ggttaggcgg ccggattctt ggttggacga ccgactgata cgtggcggaa
     2641 gccgaagggg acgacccggt ccgactcgta ggtgttgcgc aggtccagca acagtggttc
     2701 ccgcatggct ttcttgagcc gttcgaaatc gagatttcgg aattggttcc actcagtcat
     2761 gataatgagg ccgtcggccc cttccgcgac ttcgtaggtg tcctggcaag ggaccatgcc
     2821 ggggagcagt ttcatcgatt cttccatcga cgcgggatcg tacgcgcgga tctttgcccc
     2881 ttccttcatc agctcgctca ggatggttaa cgatggcgct tcgcgcatat cgttcgtgtt
     2941 gggtttgaac gaaagcccca acacgccgag ggttttcccc tttactcctc cgcaggcatc
     3001 cttcactttc tgaaccattc gcaggtgctg ttcgtagttc actttcgctg ccgcgcctgc
     3061 aatctggaac ggatacccta cacgctcacc ggtctggacc agcgccgcga ggtccttggg
     3121 aaagcaggat ccgccgaagc cgggcccggc atgcagaaat ttgttgccga tccggttgtc
     3181 gagccccatg cccttggaga ccagctggac gtccgctccc accttttcgc acacggtcgc
     3241 gatctcgttg ataaacgaaa ttttgacggc cagaaatgcg ttggaggcat acttgatcat
     3301 ctcagcagtt ggaatgtccg tgaccacgat gggcgtctcg agcagataga gggggcggta
     3361 gaggtccttc atgatcgcga cggcctgctc gctgtctgcg ccgatgacca cgcggttcgg
     3421 ccgcataaag tcttcaatgg cggagccttc ccgcagaaac tccggattgg agacgatatc
     3481 aaaccggaat cgaccggttt ggttggcctt aatcacttcg cgcagctttt cgccggtacc
     3541 gaccggcacg gtggacttgg tgacgatgac cttatacccg gtcatgttct tcgcaatccc
     3601 gcgtcccacc tcttccacgt acgacaagtc ggctgaccca tcagatttgg gaggggttcc
     3661 gacagcaatg aagatcacaa gggctttatc gacggctttg gcgacatcag tcgtgaaatg
     3721 gagccggtct tctttgatgc ctttggcgac gagttccgtg atgccgggct cgaaaaatgg
     3781 cacttcaccc ttttccagtt tggcaatccg gcgcgcgtcc gtatccatac aggtgacgtt
     3841 cacgccgaat tctgcaaagc aggccccggt gaccaagccg acatagcccg ttccaatcac
     3901 gctgatatgc atggatgcat ctcctcttaa agaaaagctg cgaatcgtgg gtgagcgttt
     3961 cgtgtagagt gcgtagtata gcaacgcatg ccagaccgag caatcatctt ggaggttttt
     4021 gcagtccgtg ccggatagcg catggcagga tgaattgcag attgtggcgt acttcagtag
     4081 aattcggcct cttcgcagga gggatgcgca tggtggttgc aggcgggccc atccaacgac
     4141 cgttggccat gatgatgcgt cccattacga agaccgtgcg acccgatgat tcgttattgg
     4201 tggtagcgca gcaattacgc gatgcccggg tcggggccat gctcgtcgct gaccatggcg
     4261 actatgtggg cattgtcagt gaagccgatc tcgttcgcaa ggcgatggcc agcggggccg
     4321 cgtcagacca ggtcacggtt cgctccgtca tgagtgcccc cctcatgacg atcgatattg
     4381 cccaatccgc gcatgaggcg agcgatgtga tggctgaacg aggaatccgg catctggtga
     4441 ttaccgatga agggcgtgtg gtggggatga tctctgttcg cgatctgctt cgatacttca
     4501 agaattgggg tactttgtag catggtgtgc tcgcccgccc gctgttgtcg cttcccctca
     4561 cggtgtcttt cggtctttgg tcgatgatgg cacaaagacc tgccgttgtt cccattcccc
     4621 gtctcttctt gctgttcacc gcgctcgtga ccggcgcgat cgtcatggcg ctcgaaattc
     4681 tgggcagccg gctgctggct cctgtcttcg gaaattccct ctttgtgtgg ggcgccttga
     4741 tcgggatcat tctcgcggcc atgagctccg gctatgcgtt cggcggttgg gcttccgacc
     4801 ggtatcgtgt tgcggcggtg ttggcgtggc tgctgctcgg atccggcgcg tggacgttgc
     4861 tgatgtcctg gatggggcag ggcgccattt tccaggttgc caaactcgtc gaggatccgc
     4921 gatggggccc cagccttgcc gcctgtgtct tgctggcccc tccggcattc ggcctgagcg
     4981 gtgtgatgcc cgcattgctc cgattagccg ttgtcgatat gggatatctg gggcgacata
     5041 ccggcagcat gattgcgtta tctaccgtcg gcagtctggc gggaacgtgg gggacggcgt
     5101 tctttctcct ttcgtggttg ggcacgcaga ccctcgtagc gacgctgggg gtaatccaat
     5161 tgctcttggg attactctgg ttgcaacaag gaagcgggag agtcacgaag ttaaccgggg
     5221 tggtgatgac cggcctgctc atatcgggtt ggcaactgtt cggccaggct cctccggtcg
     5281 caggattggt ctatcaggaa gatagcccct atcagcaagt ccgtgtccgt gacgatgatc
     5341 tgtttcgtta tctcgtgctc gatcgcacgt ggcatgcggt catgtggcga tcagacccga
     5401 ccgccctgtt tttgccctat agccaactga tggtggcggc ggtcgcattg gctccagagc
     5461 cgaaacgagg actcatttta ggacatggcg gcggctcgtt ggccaagtgg cttgctcaag
     5521 tctggcctga gctagagttg gatgttgtcg aattcgatcc ggtggtggtg cggatggcgg
     5581 aacagtattt cgattatcgc cctcctgcca atcaccatgt ctcagtgaaa gatgcgcgcg
     5641 tgtttctgcg cgacacgaaa gcgacctatg acgtcatctg ggttgacgcg tttgcccggc
     5701 atctcattcc cttccatttg acgacggtcg aattcttttc ggagcttcga ggacgtctca
     5761 accccaatgg cgtgttggct ctcaatctgg cttcttcagg ggagggtggc gatcttcaac
     5821 gtgcgaatgc cgtggtgcag acattgaaaa gctcctttcc ctcaattgaa tcatttgggg
     5881 taaagggacc gtggcgtgcg caccagacca cggcagaaaa tctcattttc ttcggtggcg
     5941 gcccgatcgc tacgatgtcg tatgacgaga tcgtgaagcg tatttacatg caggtggaag
     6001 cccgtcgatt gcccctcgaa gcgatagcct tgctggccgc gcgacgtacc acgccttggc
     6061 cgcccgggct gacactcacg gacgactaca caccgtatga cctgctgatc ggctcccatg
     6121 cagtcgaaat gtcgccagag gggaattcat cgccgtaagt ggttgaaatg tagctacgaa
     6181 gtgccgatct atcctctaca tgcgacgagc atttcctgcc taaagacccc gtcttgaccg
     6241 tccaagaact ttcttgcttc cttttaaatc cctatgctat aaggtgaccc tcatcagcct
     6301 tttatttagg acatttccga gcagtgtgtg ctagcctccg gggcgcggtg tctagcagaa
     6361 ggttcagatc gcggtggtca ttattcacca acctaatcgc atctcttctt aatctcttta
     6421 aggaggtagg tcatgggcaa gacgatggta gctgtattct ttggcctggt catgttaatg
     6481 gctggggcat ccggcgcgta tgcgctgcaa gctggcggtg tcgagatcgg cgatctggat
     6541 accggctccg tggtcggtca gccatttaaa gaattgattg tggatgcgga attgtatgcc
     6601 gtcgagatcg ggaacttgaa ggcctggtat ccaccggtca cggtgattga ttttaaagtt
     6661 cgccccggcc gtccggttgt tctcaaagtg acgaataact cctccagcga gcatggcttc
     6721 cagatgaccg atccggtcaa cgcagcatca ccgtttgtca tgaaagcgga agtggtgctg
     6781 aagcctggcg agaccaagta tatcggtgtg cctaccagcg acatgttcaa tgccacgcag
     6841 ggcaacacgc tgacctatcg ctgccatctg cacccggctc acgtcggtgg caagctggtg
     6901 atgattaagt aatctgagtt tgaacggtac gagttgcaac gccccatctg cagtgatgca
     6961 gatggggcgt tgtcgtatca agggcctgta tcgaaatcgt tcttgcctag acttcctgtt
     7021 ccttttgaca ttctgcggca gcatccagcg gaatgttttg aatgccttgc aggcgtcctt
     7081 ccacgatcaa cagcagaaac cgactctccg tcggcggcgc ctctcgccgg tctgtcacca
     7141 cggtattcat gtccacgatc atttcgtcgc cgtcccattg ggtgtcgttc accacccaaa
     7201 aatgactggc cgcatcagga aattgctttg tgatgaaact ttcgatgatc gtgccgatat
     7261 cccctacctc cgtcattgcg taggaaggca gcggtgcgag agcgaggatg gcaatacatg
     7321 caaggcgcgt gaagcagttc atgtgtacct cccgtggata gggggccaat gaatggacgc
     7381 cgcggggcca cgtaggggca agttggcctg cgcatgccac aatttactta aagtctacag
     7441 cgatcgactg cggaaagcca ggtatggatc tgttgttgca ccgctggtta gtggaaaaac
     7501 agcgaaggct cccggaggag ccttcgtggt gccacgcaca tgtactgaac ctaattagtt
     7561 acgaacgccg ctccagacct tagccggatc aatttctttg tccggattca atgacttggc
     7621 cttttccaac aatacgtcag tcgtctccgg ataggctggg tcagcaatgc agcagttgtc
     7681 caccgggcag acagccgcac attgaggctc gtcaaagtgc ccgacgcact ccgtgcaacg
     7741 atcatgggtg atgacgtaga tgttatcacc gactccctgc ccgtctccga cgtgattgcc
     7801 cttgccttca gcatcgctgc gcgtttcgaa aattgcttcg ttgggacatt cgggcaggca
     7861 tgccccgcag gaaatacact catcggtgat cagaagcgcc atggcctatg cctccttcgc
     7921 agttccttta atgacggata agatgttgac aatttcgtgc cgccgcgacc atagttcgcg
     7981 gcgaaacagg tccgcattct atgctggcgt tcttcggcaa gtcaacagaa tgaggcttgg
     8041 gaagaaggcc tagcaccagg aaagtcatgg ggctttgggc ccaatgtaca tgcggctcta
     8101 cgcaggtacg actacatgag gatgcatcca tgtccgacag catttggtta tggtgacgcg
     8161 cgcgagcggg tctgaatcgg aacagcgtcg caagaaaatt gtgacgttcg tgctcttagt
     8221 gatcttgctg atcagtgtga gcctgtttct cggcggtcca aaggaatccg aactcatcat
     8281 tgtcgaattt ccgaacggga agacgatgga gacagaggtg gccagcacgc cggaaaagct
     8341 gctgttcggg ttggccttcc gggaaggatt gccagccgat acggggatgc tttatatctt
     8401 tgaatcgacc ggtttgcacc gtgtcgcgac tcgtcaattc cgcattcctg ttgatatgtt
     8461 gtggattgat gagagtcatc atgtggtaca cattctcgag caggtccagc cctgcctcca
     8521 ggatccttgc ccgttctacg gtccgcctcc cgagccggtt cgctacttaa tcgaagcaga
     8581 ggcggggtac gcaaagcgcg caggaattac catcggtatg gagctcaagt tcacattacg
     8641 gatgtaacag taaggggagc cggaatcgtt tatggaaggg tttcagtgcg tggccaaggt
     8701 tgaagatatt cctcccggcc aagtcaaggt cgtcaaggtg aatgagcgtg ccattgcggt
     8761 ctttaatatt gaggggcatt ttcacgccat ctataattcg tgccctcatg aaggtggtcc
     8821 tttaaataaa gggcgcgtga aggggcatgt cgtctcctgt ccctggcacg acttggcatt
     8881 tgatgtgcga aatgggcaag gcacggacgg cggggggtat tgcgtcggaa gttatgatgt
     8941 gcgcgtagaa gacgggcaga tattcgtcgg agggcggcgc aaggtgtagc cgcacggaat
     9001 gatgatattg gagttgttag gaagatgagt ccactgagca aagcggtgcc gacaggtacc
     9061 gagcaggagc cgggtgccaa agtcgtgaag gcgcgagtgg gactgccggt tcatatccgg
     9121 ctttgggaag atcgcacgcg gggagagtta tgggttccga tctatgacgc cgcagtcatg
     9181 cccttggtgg aggatgattt tcttcgtacc gccagcaaca atgcggtcga tcaagggcag
     9241 cgtagtttcg agttcaagcc ctcggttgcc ggcactcatc gggtggtatt tgagaagcgg
     9301 ctggggtgga aattcacagc cgaagaccgg caaatttttt atgtcaccgc gtcctaagaa
     9361 gagggcggcg agaaaaaggc catgcctgac attgcatgcg tgaacggttg cttcggtcct
     9421 gtggcggaag ccgtagtcag catcgaggac cggggatttc agtttggcga cggtgtgtat
     9481 gaggtcattc gcacctatcg cgggaaacca tttgccctcg atgagcattt ggcccgtttt
     9541 gagcgaagtg cgcaggcgtt gcacctctcg ttggggctta ccaaagatca gtggatggcg
     9601 ctcgttcagg aaggccttcg gctgagccag ttccctgaga ccaaaatcta tctgcaggtc
     9661 acgcgagggc aggctccacg ggatcatctc tttcccgcct ccaccgtgcc aacgaccgtg
     9721 ctgacttttc gcgaactcca tccgctggat gcctcaattc gcaaggccgg ggttggagcc
     9781 gtaacagtgg atgacattcg ctggggacgg tgcgatatca agagcgtcaa tctcttggcg
     9841 aatgtgctgg cgcggcagcg cgcgaaggaa gccggtgtgt ttgaggcgat tttagttcga
     9901 gatgggcttg tcaccgaggg gtctgtcagc aatgtcatgg ttgtgcgaaa cggtgtggtt
     9961 gagactgcgc ccgaagggca ccgcattctc tcaggggtca cgcgggcgat ggtgctggag
    10021 ctggccagga aggaagggct tcccgtggtg gagacatttg tcggtcggga agatctcttg
    10081 gcggcatccg aggtgttctt gacgggaacg acggtggaag tgttgccggt tgtgcgcatt
    10141 gacgggcaac ccatcggtgc aggtgtgcca ggcccgctgt cacaacgttt gtctcgtcgt
    10201 tgggaggccc tggtcgggta gcgtcccttg ctttcactcc tatggcatgg taaggttcct
    10261 ccgctgtaag tgggcctggg cccacttttt tgtttgagca acttttgcct atgagtgatg
    10321 cgggagcggt gtcggcaggg aagcctttgg tgaaacgcgc tgaggcgctc aacctccgcg
    10381 tgcaagaagt tgcggctccc attctgcaat cccacggcct tgagttggtc gaggcggtat
    10441 gtgtcggcca aggtccccgg accgtgatcc gggttttcat cgacaagccc ggcggaattt
    10501 cgctgaccga ctgtgagcag gcgcatcgct ctctgagccc ggcgctggat gtcatcgacc
    10561 cgtttccaca tgcctatacg ttggaaattt cgtcgcccgg cctggatcgc cccctgcgaa
    10621 ccgcgcatga ttatcggcgt ttgatcggcg agccggtgac gctcaaattg cgcgagccga
    10681 ttcagtcgca gtggcggctg gtggggaccg tgctggaagt ggaggatggc gcggtgacgc
    10741 tcgctatcca gcagaaaaaa acgacggaga cgatccgggt ggagtttgat cacatcgccg
    10801 tagcgcgtcg gaaggtcgaa ttttccaagt agcagacggt gcccgctgct ctgttaacca
    10861 tgaggtgtga gctatgaacc gagagttgat cgccgtcatc gatgaaatcg gccgtcaaaa
    10921 gggaatcgat aaggccaggg tcattggcgc gatcgagtcg gcgctgcaaa ccgctgcgaa
    10981 gaaacgattc ggccaggccg agaatatcca ggtcgaaatt gatagcaaga ccggcgaaat
    11041 ttcggtcgtg tcgaagaaaa tcatcgtcga tacggtggcc aatccgaagg ctgagatttc
    11101 cctgaaagag gcgcgtgaat atgatgaggg cgcggaagtc ggtgatgaaa tcggctccct
    11161 catcgaaatg gacgaattgg gccgcatcgc cgctcaaacg gccaagcagg tgatttttca
    11221 gaaggtccgg gaagcagaat gggaagccgt tcaaaaggag tattcgaccc gtcagggaga
    11281 cttggtgaac ggcatcattc tgggcatgga gcggagaaac ttcctggtcg atctcggcaa
    11341 gacggaggcg attcttccga ttcaggagca gatcccgcgc gaaacctacc ggaggggaga
    11401 tcgcgtgaag gccttgctgc tggaggtgcg tcgcacaccg aaggacgtgc aggtcatcct
    11461 ttcacgtagc catcctcagt ttgtcgcgaa gatgttcgag ctggaagtcc cggaagtcgg
    11521 cgagaaaatc gttgaaatca agtcgatcgt gcgtgagccg ggtgatcgta ccaagattgc
    11581 ggtgtcatct cgcgacaaag cggtggatcc ggtcggagcc tgtgtcggga tcaaggggtc
    11641 gcgtgtgcag gcggtggtgc gagagttgcg tggtgaaaaa atcgacatta tcacgtggac
    11701 acaggatcct cgcgtgttca tcgcggaagc gttaaatcca gcgacgatcg aaaaagtcgg
    11761 catcgatgaa gaaaagaagt cggcactagt ggtcgtggcg gattcccagc tttcgctggc
    11821 gatcgggaag aacggacaaa atgtccgtct ggccgcgcgt ctgacgggtt ggaagatcga
    11881 tatcatcagc gcgactgaat acgagaagga aaaggccgag cgggatcggg acattaaggc
    11941 ggccttggcc gaagagaccg aggctcagcg ccagcaggat gaggcacgag ctgcggcgca
    12001 gcaagcggag taaaagggct cgcaactcac gagctgaatg gcaattatct aagcgggtgg
    12061 agctgagcgg tactgtatgc gcgtgtacga attagcaaag cagctgggga tggaaaatcg
    12121 ggagctgatt cctgaactga a
//
LOCUS       DAOQEW010000114        11888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1588_length_11888_cov_8.142314, whole genome shotgun sequence.
ACCESSION   DAOQEW010000114 DAOQEW010000000
VERSION     DAOQEW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_11888_cov_8.142314"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..974
                     /locus_tag="PLS35_12515"
     CDS             222..974
                     /locus_tag="PLS35_12515"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNI19536.1"
                     /translation="MAQKRLTRAQFLERLLSIMDRKHHWAWPIIMGPGITKAQLKLHY
                     QQEYLVYVRDFPVLLARVHGQNPPPDVRRMLAENIYEEDTGGLSFGRSHPELFNEMMR
                     GLGFDSRVFLRATLLPASLRYRRWLEKVTASRDWVVGAAALAVFVEGSIKDRQEIQEP
                     SKPKTEAQIEAYIDAHPLIRHHKIEREHMDLIRAHQKVEAGHRQDAYTMVVNYAETRS
                     QQNAVLACVRQGLTLWAAYRDSIAKACKLTKP"
     gene            971..1867
                     /locus_tag="PLS35_12520"
     CDS             971..1867
                     /locus_tag="PLS35_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI19537.1"
                     /translation="MRRLKATPRWFLLCLLVMLSLYQAAWASLSSDDMVLVPAGEFTM
                     GASVDRGGLPDEQPIRLVYLGSFLIDRYEVTNAAYLQFVQATNYQAPANAADRLTLWE
                     QNRPLPGIEQHPVVNVSWLDAVAFCRWANKRLPTEAEWEKAARGTDGRTYPWGNDWSL
                     DKANSASYWAGKTVEFADSREWEAFWIKGLGAELSKAKGLKGEILTLPVGSFPGGASP
                     YGALDMAGNAAEWVQDWYNPNHYRSASLTNPQGPERGAIKAMRGGSWLKPAISLRTSD
                     RDWGTMDSRPSGTGFRCARDSY"
     gene            2033..2785
                     /locus_tag="PLS35_12525"
     CDS             2033..2785
                     /locus_tag="PLS35_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19538.1"
                     /translation="MASQLSRASDTGSEPICRVLLADDSRESQALIQLYLRGLPYQLD
                     VVGNGKEAVAMFQSQQFDLVLLDQHMPVMDGFTAARVMREWEASNERAPVPILTLTAD
                     SLVDVKTQAQAAGCTACLAKPIVKEQLLDAFRRFGARPSPKKPVVTQNNSPAGMAALI
                     DEEITRRRPLFLDHRRQDLKRMQEAIEQGDFDAIRIMGHRIKGLAGSYGFHDIGLAGA
                     QLEQAAREQDIAAMRRTIDRLTTLLTQARQAV"
     gene            2813..3457
                     /locus_tag="PLS35_12530"
     CDS             2813..3457
                     /locus_tag="PLS35_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19539.1"
                     /translation="MSILIIDDFQEERDLLHAILHRAGFGPLLPVATAQEGLTLLGIG
                     KRGKPGNGIDLVLMDLEMPGINGLEACEKIRMEEQLQGLPIIIITAHTAEKDIQAAYT
                     AGATDYIRKPVIPVELLARAATALSLKQEIDARKLREQELLERTKELDHAFEQMTTLH
                     GTLHICAKCKRVKTDGSYWQRIEDYLRRLARFTVKEAVCESCMHQAYPHLKRIP"
     gene            complement(3496..4161)
                     /locus_tag="PLS35_12535"
     CDS             complement(3496..4161)
                     /locus_tag="PLS35_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19540.1"
                     /translation="MRTHLAMALLVLAWLMPATVSAEIPSPLRPLHTTLSAALATLPS
                     DSHLLLDREPIEQFLAALDGSPPDWPQIYGQGHHDPGHDERLFALNRERDARREGKEP
                     LQWLVTVVWLGEVSRFDEEEGGFRVALGPKFNRTAWGEVRFKHEDLPATLIASAGGAT
                     DDLKARMQRGERIDIDVVMCGRLIPEESLVYDFSHEVDGLGLIMPVVRIEAVAFVLAD
                     GSR"
     gene            complement(4181..4501)
                     /locus_tag="PLS35_12540"
     CDS             complement(4181..4501)
                     /locus_tag="PLS35_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19541.1"
                     /translation="MMNDQEEQLRRAIGTLLQSDPLIKLLQEVRLGRMKATDAGLRAV
                     VESWIGTYEQVLKSQSLNAELLARLDPVPRLQVLVDAGVLSWEHSATCELRDLFRGLL
                     VPAA"
     gene            complement(4529..4756)
                     /locus_tag="PLS35_12545"
     CDS             complement(4529..4756)
                     /locus_tag="PLS35_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNI19542.1"
                     /translation="MPIFEYVCQECNHRFELIVQGQTVPACPSCKGTTLDKQFSAFGV
                     GATGGWPVVSNSGGGCGSCGDPRGPGACSMN"
     gene            4836..5090
                     /locus_tag="PLS35_12550"
     CDS             4836..5090
                     /locus_tag="PLS35_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19543.1"
                     /translation="MWSEDKSFVFRISLEAQFPDDYDGPLDEQAWLREWELQIKPVLI
                     KNLFDTLREYPAWKSHVRNRGKSPTEEIEVAMIRDFSPTP"
     gene            5106..6257
                     /gene="hemW"
                     /locus_tag="PLS35_12555"
     CDS             5106..6257
                     /gene="hemW"
                     /locus_tag="PLS35_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNI19544.1"
                     /translation="MSQATAQGLYIHIPFCHQRCHFCAFYLEIHQARAAEEFVSALLT
                     EVRVYGESGVLRQDPLDSIYFGGGTPTTLSSQQLATLLATSAQVFGLAPDAEVTIEAH
                     PGSVSRDSLAHLREAGCTRLSFGAESMDQTELDRVGRPGSPSTTLHVMAMAREAGFTN
                     INLDLMYGLPGQTLESWATSLRQTIELAPEHLSCYALTIEEGTHLKSSIQRGLLPAPD
                     EELQNRMEDLAEEILGRAGYDRYEISNYCRPGCASRHNRLHWTGGHYLGVGPSAQSYI
                     GGRRFGNVSDLTAYKAALRNGRLPVSESDQLTQAGESCERLIFGLRLVDGISLTQTGA
                     LSFPELSREVDKLLADELLERKGDLVRLTTRGRRYADTVAVSLLTSLDE"
     gene            6331..6528
                     /locus_tag="PLS35_12560"
     CDS             6331..6528
                     /locus_tag="PLS35_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19545.1"
                     /translation="MPVNMDPSKRRRRQPVESTASAADSETTQPKRFGEDTEADREKA
                     IAEAELKNLWDATEVIDMDSW"
     gene            6614..7306
                     /locus_tag="PLS35_12565"
     CDS             6614..7306
                     /locus_tag="PLS35_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19546.1"
                     /translation="MTSLPRRHALFYPFHLCAPGTLEYLLEHYGSLHFRDYMALRLTP
                     FMGTTAYQDRMGDEHPDLVSNGRIVQGYAVSGPLDDTAVAEIDRDFSDPAWRGLFHAG
                     LQADRRFQRGLFDLTHAMRIGSTLVPGPAALLRLLEPQRATEVCTVGLVKHLARSSAT
                     LEDAYQFEYGLALVKTAAAQVYTFRLARAHGLVPVTDSKTHYRLLSHTMAREGIDLPN
                     EYVVPSQSPAAP"
     gene            7434..7727
                     /locus_tag="PLS35_12570"
     CDS             7434..7727
                     /locus_tag="PLS35_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNI19547.1"
                     /translation="MSTLTPVMTPDALDTTDVGTGDDLEARVIVFNCECHTYQQVIAL
                     FCKCIPGMNSSRAFELAWRIDHEGQATVYSGARKVAEEIGTKLAAGGLRVGVQ"
     gene            complement(7782..8129)
                     /locus_tag="PLS35_12575"
     CDS             complement(7782..8129)
                     /locus_tag="PLS35_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19548.1"
                     /translation="MERRAASHTEEEEMNQRRRLLGLARKGDPKAISKLFELYQVRVL
                     NGDMLSKLNKSYYKAAAAQEQKAAQTPAKSAKGSQATHGSAKGSAKKSAAAAAPATKA
                     KPTKAATPKKRAK"
     gene            8373..9050
                     /locus_tag="PLS35_12580"
     CDS             8373..9050
                     /locus_tag="PLS35_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 domain-containing protein"
                     /protein_id="HNI19549.1"
                     /translation="MEHVAAVDTQPTVADDPQARAILQRAFENTARWQPDFRGFSADL
                     SVNTNGQVVSGTVVVKGPREVSVQLPDEAIQKWAQEQIGMIAVHRAPRKFEESDGKHR
                     LTMESGEGHPLGRRLDIHGDGMQSFYRIKDNRITQINRKMPHVAFTINVEESSTTQDQ
                     KQLTTKYTVYYFAPKDSTLRNVESFTDTHVRIGASDLPAARRIISYENGAVLVRTLTF
                     TNHKLLA"
     gene            9050..9481
                     /locus_tag="PLS35_12585"
     CDS             9050..9481
                     /locus_tag="PLS35_12585"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNI19550.1"
                     /translation="MDLRKTFPRSMKTRLAGYVHLARMIDKCRAVLAGTEGEYIYPCP
                     MDERLLEYAGITAEQFTAAVKTNVTDDGVAEWFRRTATPHRAADLEDWNGKLLGRGPS
                     SPESAAKFAKYRDAIDPSRTDITAWSDLQDLEEGRPVPRRG"
     gene            9780..10376
                     /locus_tag="PLS35_12590"
     CDS             9780..10376
                     /locus_tag="PLS35_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19551.1"
                     /translation="MALVQQNLVPGTGDHLITLDTETNLEWLSLNATANLSYMDVLGG
                     AGGYTTTYGFRYATGQEISLLHQHAGITKFGLTQVVPFPQSNHVAMETLIELMGGVAL
                     YPSVASGSVLVQTQGMMKFRGTGIPAPMTPMSVVQLWLFKHNPPSSYADSNPAGPAGN
                     RAPEIASYLIRNRVMPVGVVARGKSAKTVKTPRAKKKS"
     gene            10681..11238
                     /locus_tag="PLS35_12595"
     CDS             10681..11238
                     /locus_tag="PLS35_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI19552.1"
                     /translation="MTTTAPHDQSRFAPGHASNRSVSPKNILIAVDDSNATIRVLHYV
                     GALLRETPAVAITLFHVLKPMPRELMEHGGSENPDMEHQLGEQLRKDQQEWMQMEETL
                     EYPILERALERLKDTGFPTERVMLKLGYAGDLVDTILDEVRAGGYGTLVITKHSQPDA
                     AHLFSNNVAERLSRELSGIALWVME"
     gene            11430..11816
                     /locus_tag="PLS35_12600"
     CDS             11430..11816
                     /locus_tag="PLS35_12600"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNI19553.1"
                     /translation="MKDETIPAAVRQYFEAIGKMDADSWVSCFAEEGISYEPGAPAPL
                     KGRAALRQFLVGVLGAFETIRMTADHVFQSANRVAVKFTGRGTGKNGRPVFFEGIDVF
                     EINQEGKIQTMWGYWNPATMMTQLQG"
BASE COUNT         2635 a         3565 c         3299 g         2389 t
ORIGIN      
        1 cttatagaat gcgtccgggt cttcaccgga ggcattgagg taaatcggct ccgtaaaccc
       61 ttcttccagc acatagtcca acagacagtc cgtggtaaac ccttttccat gcagggcaac
      121 ctcggcaagg aagttgagaa tctcgtcgga atcctgaatc gtgataggct tcatctgacc
      181 ggtggctgat tgtagccagt aggaacgaag gaaggcaagg catggcacag aagagattga
      241 cccgtgcgca attcctcgag cggctcctgt ccatcatgga tcgcaaacac cattgggcct
      301 ggccgatcat catgggaccc gggatcacta aggcccagct aaagctccac tatcaacagg
      361 aatacctggt ctacgtgcgg gacttccctg tcctgctcgc tcgagtgcat gggcagaacc
      421 cgccccccga cgtccggcgc atgttggccg agaacattta cgaagaagat accgggggcc
      481 tgtccttcgg gcgatctcat cccgagctat ttaacgaaat gatgcgcggg ctggggttcg
      541 acagccgggt cttcctgcga gccacgctcc ttccagccag cctccgctat cggcggtggc
      601 tggaaaaggt cacggccagc cgggactggg tggtcggcgc cgccgccctt gcggtgtttg
      661 tcgagggcag tattaaagac cgtcaggaga ttcaggaacc ctccaagcca aaaaccgagg
      721 cccagatcga agcctatatc gacgcccatc ctctcattcg tcatcacaag atcgagcggg
      781 agcacatgga tctcatccgc gcgcatcaga aggttgaggc ggggcatcgc caggacgcgt
      841 acaccatggt cgtcaactat gccgaaacga ggagccagca gaatgccgtg ctggcttgcg
      901 tgaggcaagg gctcacgctg tgggcggcct atcgcgacag catcgcaaaa gcctgcaaac
      961 tgacgaagcc atgaggaggc tgaaggctac ccctcggtgg tttctgctct gcctgctcgt
     1021 catgctcagt ctgtatcagg cagcctgggc atccctttca tccgacgaca tggtgctggt
     1081 tcccgccggc gaattcacga tgggagcttc cgttgaccgg ggcggcctcc cggatgagca
     1141 accgatacgc ctggtctatc tcgggtcgtt cttgatcgat cggtacgagg tcacgaacgc
     1201 cgcctatctg caattcgtcc aggccacaaa ctatcaggcc cccgccaatg ccgccgatcg
     1261 cttgactcta tgggaacaga accggccact tcccgggatt gagcagcatc cggtcgtgaa
     1321 tgtgagttgg cttgatgccg tcgcattctg ccgctgggcg aacaaacggt tgccaaccga
     1381 agcggaatgg gaaaaggccg cccgggggac cgacggacgg acctacccct ggggcaacga
     1441 ctggagcctc gacaaggcga acagtgcgag ctactgggcc gggaaaaccg tagagtttgc
     1501 ggatagcagg gagtgggaag cgttctggat caaaggcctg ggcgcagaac tctccaaagc
     1561 gaagggtctg aagggggaaa tcctcacgct gccggtcggg agttttcctg gcggcgccag
     1621 cccgtatgga gcgctggaca tggcaggcaa tgccgccgaa tgggtgcagg actggtataa
     1681 ccccaaccat taccgctccg catcgctcac gaatccccaa ggaccagagc gtggcgcgat
     1741 taaagctatg cgcggcggat cctggctcaa accggcaatc agcctgcgta cctccgatcg
     1801 tgattggggg acgatggaca gccgcccttc cgggacggga ttccgttgcg ccagggatag
     1861 ctactaatcg aagggctgtt tctccagcct cgcccctgcc cgaggtctct gagcgatcga
     1921 ttcctcatac ctgccgggtt ttcaggagat cctaggtcgt ccaggcttca cccgctatac
     1981 tacttctaag tactgtcacg tccgcatcac gttgatccgt cgagcgtcgg ctatggcatc
     2041 ccagctttct cgtgcctcag acaccggctc tgaaccgata tgccgggtct tgctggccga
     2101 tgactcgcgg gaatcacagg ctttgatcca actgtatctt cggggcctcc cctatcagct
     2161 tgacgtggta gggaatggaa aagaagcggt cgcgatgttc cagagccaac agtttgatct
     2221 tgtccttctg gatcaacaca tgccggtcat ggacggtttt accgccgcgc gcgtgatgcg
     2281 ggagtgggaa gcatccaatg aacgggcgcc agtccctatt ctgaccttga ccgccgactc
     2341 actggtcgac gtgaagacac aggcccaagc agccgggtgc acggcatgct tggccaagcc
     2401 cattgtcaaa gaacagctcc tggacgcatt ccggaggttt ggcgcaagac cttctccaaa
     2461 gaaaccagtc gtgacacaga acaactcgcc cgcgggcatg gcagcgttga tcgacgaaga
     2521 aattacgcga cgccgtcccc tcttcctcga tcatcgccgg caggacctga aacgcatgca
     2581 ggaggctatc gagcaaggag acttcgacgc catccggatc atgggacacc gcatcaaagg
     2641 tctggccggc tcgtacggat tccacgacat cgggctggcg ggagcccagc tggaacaggc
     2701 agcgagggag caggacatcg ccgctatgcg ccgaacgatc gaccgcctga ctacgcttct
     2761 tactcaagcc aggcaggccg tttaagcgat gccgtagaga aagcttgcca gcatgagcat
     2821 tcttatcatc gacgattttc aggaggaacg agacctcctc cacgctatcc tgcatcgtgc
     2881 cggattcggc ccactccttc cggtcgcgac ggcacaagaa ggactcaccc tcttaggaat
     2941 cggcaaacgt ggaaagcccg gaaacgggat cgatctcgtc ctcatggacc tcgaaatgcc
     3001 cgggatcaat ggattggagg cctgcgagaa aatcaggatg gaggaacagc ttcagggttt
     3061 gcccatcatc atcattaccg ctcatacggc ggagaaagat attcaggccg cgtatacggc
     3121 cggcgccacg gactatatcc gcaagcctgt gattccggtc gagttgctgg cacgagccgc
     3181 cacggcattg agcctgaagc aggaaattga tgcgcggaaa ttacgtgaac aggaactgct
     3241 tgaacggacc aaagaactcg accatgcctt cgagcaaatg accacgctcc atggcactct
     3301 tcacatttgc gccaagtgta agcgtgtgaa gacggatgga tcctattggc aacggatcga
     3361 ggactactta cgccgtctgg cgcgcttcac ggtcaaggaa gctgtctgtg aatcctgcat
     3421 gcatcaggcc tatccccacc taaagcgaat accctagccg ttccacgtct caactaccag
     3481 acgatattcc agccattatc gcgagccgtc cgccaggaca aatgccaccg cttcgatacg
     3541 cacaaccggc atgatgaggc cgaggccgtc gacctcgtga gaaaaatcgt agacgagtga
     3601 ctcctcagga atcagcctgc cgcacatcac cacgtcaatg tcgatccgct cgccccgctg
     3661 cattctggcc ttgagatcat ccgtcgcccc acccgccgag gcgatcagtg tggccggaag
     3721 atcttcatgt ttgaaacgta cctcgcccca tgccgtccga ttaaatttcg gccccagcgc
     3781 gacacggaat cccccctctt cttcgtcaaa ccgtgatact tcgccaagcc aaaccacggt
     3841 cactagccat tgcaagggtt ccttgccttc gcgccgggca tcacgttcac ggtttaaagc
     3901 aaacagccgt tcgtcgtgac cgggatcatg atgcccctga ccgtagatct gaggccagtc
     3961 gggaggcgac ccgtccagcg ccgcgagaaa ctgttcgatg ggttcacgat cgagaaggag
     4021 atggctgtca gaaggaaggg tcgccagcgc tgcggacagg gtcgtatgta acggacgcag
     4081 cggagagggt atttcggccg aaacggtagc cggcatcaac caggccagca ccagcaaggc
     4141 catggcaagg tgggtacgca cctggagcgc actcctcacg tcaggcagca gggaccagca
     4201 ggccgcgaaa taagtccctg agctcgcatg tggcagaatg ctcccaagac aataccccgg
     4261 catccaccag cacttgaagc cggggcaccg ggtcgagtcg agccagcagt tcggcattga
     4321 gagattgaga tttcaacacc tgctcgtagg tcccgatcca ggattcaacg accgcccgta
     4381 atccagcgtc tgtcgctttc atccgcccca gccggacttc ctgcaacaat ttgatcaacg
     4441 ggtctgactg gagcagcgta ccgatcgcgc gacgcaattg ttcttcttga tcgttcatca
     4501 tgaataactc gaatgaggtc gcgtcgtgct aattcatgga gcaggcgccc ggaccccgcg
     4561 gatctccgca ggagccgcag ccgccgccgg aattcgagac gaccggccat ccacctgtgg
     4621 ccccgacccc gaatgccgaa aattgtttat ccaacgtggt ccccttgcag gaagggcagg
     4681 ccggcaccgt ctgtccctgc acgatgagct cgaagcggtg gttgcattcc tggcagacgt
     4741 attcgaagat gggcatgggc tgtactcctt ggattttgaa atattataag taaggccaat
     4801 tcactttcgc aatcacagcc gacaggagtc cacacatgtg gtcagaagat aaatcctttg
     4861 tatttcgcat cagcctggaa gcgcaattcc ccgacgacta cgacggccca ctcgatgagc
     4921 aggcctggct gcgtgagtgg gaactgcaga tcaagccggt cctgatcaag aacctgtttg
     4981 acacgctgcg ggaatacccc gcctggaaat cgcatgtcag aaaccggggg aaatcgccga
     5041 cggaagaaat tgaagtcgcc atgatccgcg acttttcacc aactccatag gcacacgagc
     5101 cggctatgtc ccaggcgacc gctcagggtc tctacattca cattcccttc tgtcaccagc
     5161 gctgtcattt ctgcgctttt tacctggaaa ttcaccaggc gcgcgctgcg gaagaatttg
     5221 tctcggcgct gttgacggaa gtgcgtgtgt acggcgagag cggcgtattg agacaggatc
     5281 cactggattc catctatttc ggcggcggga cgcccacaac gctctcctcc cagcaactcg
     5341 cgacactcct cgcaacaagt gcgcaagtgt tcggactggc tccggacgcc gaggtcacca
     5401 ttgaagccca tcccggatcg gtttcacggg acagcctggc gcacctcagg gaagccggat
     5461 gcacacgcct gagctttgga gccgaatcga tggatcaaac ggagctcgat cgcgtcggac
     5521 gcccgggctc tcccagcacc actctccatg tcatggccat ggctcgcgaa gccggcttca
     5581 ccaacatcaa tctcgatctc atgtacggac tcccgggcca gacgctggag agttgggcca
     5641 cctcgttacg gcagactatc gagctggcgc ctgaacacct gtcgtgttac gccctaacca
     5701 ttgaggaagg cactcacctc aagtcatcta ttcagcgcgg cctcctcccc gcccccgacg
     5761 aagagcttca aaaccgaatg gaggacctcg ccgaagaaat tttgggacga gccggttacg
     5821 atcgctacga aatttccaac tattgccgac ctggatgtgc cagcagacac aaccgcctgc
     5881 actggaccgg cgggcattac cttggcgtcg gccccagtgc gcagtcctat attggaggtc
     5941 ggcgattcgg caatgtgagc gatctgacag cctacaaggc agccctgagg aatggtcgtc
     6001 tccctgtgtc ggaatccgat cagctgacgc aggcgggcga aagctgcgaa cgactcatct
     6061 ttgggttgcg cctcgttgat ggcatttccc ttacacagac cggagccctc tcatttcctg
     6121 agctctcacg ggaagtggac aaattgctgg cggacgagtt actcgaacgc aagggcgacc
     6181 tggttcgcct caccacaagg ggcagacggt atgccgatac ggtggcggtg tctcttctga
     6241 cgagcctgga tgagtagtga gcctcgccta tcccattgac ttccctcccc gacacagggg
     6301 agactgaaat gaaaaaggag acttcttccc atgcccgtca atatggatcc cagtaaacgc
     6361 cgccgtcgcc agccggttga atccactgca tccgccgccg attcagagac gactcagccc
     6421 aaacgattcg gtgaggacac ggaagcggat cgcgaaaaag cgatcgcgga agctgagttg
     6481 aaaaatttgt gggacgcgac cgaagtcatc gatatggata gctggtaacc ttcttcgcac
     6541 aaatcatcgc ttcgatcttc atttgcaggc ctctctccga gtcgatatcc cagtcactgg
     6601 ataagcccat cgtatgactt ccttgccccg tcgtcacgcc ctgttttatc cgtttcatct
     6661 ctgcgccccg ggcacgctgg aatacctgct cgagcactat ggatcgctcc actttcgcga
     6721 ctacatggcg ctccggctta caccattcat gggaaccaca gcctatcagg atcgcatggg
     6781 agacgagcat ccggacctcg tcagcaacgg tcgcatcgta cagggctatg cggtcagcgg
     6841 ccccctggat gacaccgccg tagccgagat cgatcgtgat ttttctgatc ctgcttggag
     6901 agggctattc catgccggat tgcaggcaga tcgacggttt caacgtggcc tgtttgatct
     6961 gacccatgcc atgcggattg gatcaactct cgtgccgggg ccggccgccc tgcttcggct
     7021 tctcgaaccg caacgcgcta ccgaagtgtg taccgtgggg ctggtgaaac acttggcacg
     7081 ttcgtccgca acgctggagg acgcctacca atttgaatat ggtctcgcgc tcgtgaaaac
     7141 cgccgccgcg caggtctaca cctttcgcct ggcccgcgcg catgggttgg ttccggtgac
     7201 ggattccaag acccactatc ggttactgag ccacaccatg gcccgggagg gcatcgatct
     7261 gcctaacgag tatgtcgtgc catcccagag ccccgcagcc ccatgacgac gtcacgctat
     7321 ccaagtaaga tatttgtccg acgtctgcgg cagcgaaggt caagagaggc tgacaacctg
     7381 ttgcactcgc ccccgtgtca cctggtacac tcggcgccga acgagacgac cgcatgagta
     7441 ccctcacccc cgtcatgacc cccgacgccc tcgacaccac cgacgtgggc acgggcgacg
     7501 atctggaagc ccgcgtgatc gtgtttaatt gcgaatgcca cacctaccag caggtcattg
     7561 ccctgttctg caagtgtatt ccaggaatga actcttcccg ggccttcgaa ttggcatggc
     7621 gcatcgacca tgaaggccag gccacggtct attcaggagc ccggaaggtg gctgaagaaa
     7681 tcggaaccaa acttgcggcc ggaggattgc gggtgggtgt tcagtaagat gattcgagaa
     7741 gagggccggc ggtcttcgcc gaccctctcg acacagataa attacttcgc tcgttttttc
     7801 ggtgtggcgg ctttcgtagg tttcgctttg gttgccggcg cagccgccgc ggcagacttc
     7861 ttggcactcc ctttggcgct gccgtgagtc gcttgcgagc cctttgcgga cttggccgga
     7921 gtctgtgcgg ccttttgctc ctgcgcagcc gccgccttgt agtaggattt attgagctta
     7981 ctcagcatat cgccgttcag cacacgcact tggtatagct cgaacaactt gctgatggct
     8041 ttggggtcgc ccttgcgagc cagtcccaac agcctccgcc gttggttcat ttcttcttct
     8101 tcagtatgag acgccgctcg ccgctccata gcgctccttt caaggaatgc cgaaacttct
     8161 gtcacacatt cgtcaaattg ccggatgaaa tgccccgcag tataacagat tcggcctagc
     8221 cagtactaca caaaccttgg tcaaatgaga gaaattttgc cggccggcaa aacgggattc
     8281 ttgcgcaaga tcatgtcctc cacagttgta gatgatcgat caacgtctct ataattattt
     8341 tttatccctt ccacgacaca aaggagcctc cgatggaaca tgtcgccgct gttgatacac
     8401 aaccgaccgt tgccgatgat cctcaggcac gtgccattct tcaacgcgcc ttcgagaata
     8461 cggcccggtg gcaaccggat tttcgaggtt tttcggccga cctcagcgtc aacaccaatg
     8521 ggcaggtcgt gagcggaacc gtcgtggtca agggaccgcg ggaggtgagc gtccaactcc
     8581 ccgatgaagc gattcagaaa tgggcgcagg agcagatcgg catgatcgcc gtgcaccgcg
     8641 caccccgcaa gttcgaggaa tcagacggaa aacaccggct gacgatggag agcggcgagg
     8701 gacatccgct gggccgccgg ctcgacattc acggcgacgg catgcagtcc ttctatcgca
     8761 tcaaggataa ccggattacc cagatcaacc ggaagatgcc gcacgtggcc ttcaccatca
     8821 atgtggaaga gagcagcacc acccaggacc agaagcaact gaccacgaag tacaccgtat
     8881 attatttcgc accgaaggac agcacattgc gcaatgtcga aagctttacg gatacacatg
     8941 tgcgcatcgg cgcgtctgat cttccagcgg cacggcgcat catttcgtat gaaaacggcg
     9001 cggtccttgt ccgaaccctg acattcacca accacaaatt gctggcctga tggatttgcg
     9061 gaagacattc ccccgtagca tgaaaaccag gctggctggc tatgtccacc tggcgcggat
     9121 gatcgacaag tgccgcgcgg tgctggccgg cactgaaggg gagtacattt atccctgccc
     9181 gatggatgaa cggctgctgg aatatgcggg cattacggcc gagcagttca cggctgccgt
     9241 gaagaccaat gtgaccgatg acggggtggc ggagtggttt cgacgcaccg ccactccgca
     9301 tcgagcagcg gatcttgagg actggaacgg gaagttgctt gggcgcggtc cgagttcgcc
     9361 ggaaagtgct gcgaaatttg ccaagtaccg cgacgcgata gatccttcgc ggactgacat
     9421 tacggcctgg tctgatcttc aagacttgga ggaaggcagg cccgttcctc gcagaggata
     9481 gtgccttgat cagtcaatcg atcaccacgc atggattgat ctcctcccat acccttgcct
     9541 tctttggcac cgaactccta atcgtatccg aatagattcc tcactgtatc cctcatgata
     9601 cgctccggtt ctgtatgtga ggcgagtaca cggctatacg tgccacttcg tactggcatc
     9661 accattccag ggtctgttca gggcgcattc gcacgaatcc attctggcac gcgaatggca
     9721 caagacatgc gtgaatcgcg ataccccatc attccaacca tcggccagga ggtacgtaga
     9781 tggctctcgt tcaacagaac ctggttcccg gcaccggcga tcacttgatc acactcgaca
     9841 ctgagacgaa tctcgaatgg ctcagtctga acgcgacggc aaacctgtcc tatatggacg
     9901 tcttgggcgg cgctggtggg tacaccacca cctacggctt tcgctatgcg acgggacaag
     9961 aaatcagctt gcttcaccag catgccggca ttacgaaatt tggcctcacg caagttgtgc
    10021 cctttccgca gagcaatcat gtcgcgatgg aaaccctcat tgaactcatg ggcggcgtcg
    10081 cgttgtatcc gtcggtcgcc agcggatcag tcctcgttca aacgcagggg atgatgaagt
    10141 ttcgtggaac cggtatcccc gcaccgatga cgcccatgag cgtcgtacaa ctctggctgt
    10201 tcaaacataa tcctccgagc tcctatgcgg acagcaatcc ggcaggacct gccggcaacc
    10261 gggcgcctga aatcgcgtca tacctgatac gcaatcgcgt catgcccgtc ggtgtcgttg
    10321 cccgtggaaa atcggccaag actgtcaaaa cacctcgagc caagaagaag tcctgattcc
    10381 atccctcgtt cccgtcccat gaagccaatt ccccggcttc atgggacgca cgcgatcacg
    10441 caggagcgcg gatctcccgc tttctctcac tccgcgctcg tgattgattt gttggcagga
    10501 gaagcatagg cttatgccta tgcggaccct gtgcatcccc agcaggtgcc gtacggttcg
    10561 cgctccagcc cgtctctctg agggcaccat gaagcctctt cgggccacag aaccagcctc
    10621 tccctcaaag atggtcacag ccacaggacc ggcacgcacc aacccaggta tggtgacatc
    10681 atgaccacaa ctgcgccgca tgaccaatcc cgcttcgccc cgggccacgc ttcgaaccgt
    10741 tccgtctctc ccaaaaacat cctcatcgcc gtcgacgatt caaacgccac catccgtgtc
    10801 ctccattacg tcggagcatt gctccgcgag acaccggccg tggccatcac cttgtttcat
    10861 gtgttgaagc ctatgccgcg ggaattgatg gaacatgggg gctccgaaaa tcccgacatg
    10921 gaacatcagc tcggcgaaca actgcgcaaa gatcagcaag aatggatgca gatggaggag
    10981 acccttgaat atcccatcct tgagagagcg ctcgagcgac tcaaggacac aggctttccc
    11041 accgagcggg tgatgctcaa attgggatac gcaggtgatc tcgtcgacac cattctcgat
    11101 gaagtacgag cgggcggcta tggcaccctc gtgatcacga agcacagcca accggatgcc
    11161 gcgcacctct tcagtaacaa cgtcgcagag cgtctctctc gggaactctc cggcatcgca
    11221 ctctgggtca tggaataggc tcagaccgcc aagcgagcca cgccacaatc caggtggcgg
    11281 aaccaactgg ttcccgcctt gtcgttcgtc acaccccgtc catttgcact catcgatttc
    11341 aatccaacgg gcgaattcct tcgtagtatg aacagcgctt gaaaattcgc cggatatgac
    11401 cgggcctatg agggagagga gaaaaaacga tgaaggacga gacgattcct gcggcggtgc
    11461 ggcaatactt tgaggccatt gggaagatgg atgcagattc gtgggtcagc tgcttcgccg
    11521 aggaaggtat cagttatgaa ccaggagcgc cggctccgct aaagggacgt gcggcgttgc
    11581 gacaatttct agtcggggtg ctcggtgcct ttgaaaccat caggatgaca gctgaccacg
    11641 tattccagtc tgcgaacaga gtcgccgtta aattcacggg acggggtacc ggcaaaaacg
    11701 gtcggccggt tttcttcgag ggtatcgatg tgttcgagat taatcaggaa ggaaaaatcc
    11761 aaaccatgtg gggctactgg aatccagcca ccatgatgac gcaactgcag ggataaagcg
    11821 acagccagcc gtctcttcgt tcatgtcgca tcaagcctga ggtggactgg attgggctcc
    11881 cactgcgc
//
LOCUS       DAOQEW010000115        11733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1622_length_11733_cov_6.996660, whole genome shotgun sequence.
ACCESSION   DAOQEW010000115 DAOQEW010000000
VERSION     DAOQEW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_11733_cov_6.996660"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLS35_12605"
     CDS             <1..707
                     /locus_tag="PLS35_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI19554.1"
                     /translation="TDTKLRVGYSTGFRAPSMNELYFPNFGNPNLGPEKNQSMDAGID
                     QYFFSKQLKLSGGFFWNHYRNLIVTTFDPGFCAPFSTFGFCPQQLGEASTKGWEAGLT
                     YTYSSDRPFLKGVVVQAQYTNTITRDLDTQTRLPRWPTDQWSASVSYQPIDPLWITVI
                     GRYVGSRFNTTGDRQAMPAFDVWSLAVTYDVTKQMQAYLRAENLFNEKYEEIASAGTP
                     IRSIFGGLRVTFGGKS"
     gene            732..2117
                     /locus_tag="PLS35_12610"
     CDS             732..2117
                     /locus_tag="PLS35_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HNI19555.1"
                     /translation="MPRPRLVIAGTHSGVGKTTVTLALMAALKARGLVVQPFKAGPDF
                     IDPGHHQLVTGRPSRNLDGWMLNEAVNRSIFSQASADADLSIIEGMMGLFDGSSPIHE
                     QGSTAELAKQLHAPVLLVIDGSAMARSAAAMAAGYARFDPGLSVQGVLFNRVRSDGHY
                     RLLQEAVEAETGLAVVGYLRPDASVTIPDRHLGLRTAIEAGSCDLYDRLAQSAAETID
                     LARVEAIARSAEGWFEEDPVSPPAAGPKETVRVGVAYDGGFCFYYQDNLELLAAAGAE
                     IVQFSPLHDAALPDVDLLYFGGGYPELYGEALAANVSMRAAVKAFSDRGGVIYAECGG
                     LMYLTQAIRDGDGHRYEMVGVFPAEAVMRRSGMTLGYRIVETMRPSLLGPAGTSVRGH
                     EFHYSLLEPKGELAYACVLADAAGKPVGQDGVMVRNTLALYSHQHFRSRPEVVVNLLQ
                     SVRQEKAARQA"
     gene            2130..2729
                     /gene="cobO"
                     /locus_tag="PLS35_12615"
     CDS             2130..2729
                     /gene="cobO"
                     /locus_tag="PLS35_12615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNI19556.1"
                     /translation="MTDRDEHTAKMQRLKGSVDRRIEAAQEEKGLLMVYTGAGKGKTT
                     AALGMAIRCLGHGMKVAIVQFIKGAIDTAEERILRSFGDRVTFLRMGEGYTWETQDRE
                     RDTRFAQEAWNKACEFMTDPSYAMVILDEFNIALHHHYVELAEVLPRLQARPLMQHVV
                     ITGRGAPAELIDAADLVTEMKQVKHPFRQGIKAQAGVEF"
     gene            2738..2941
                     /locus_tag="PLS35_12620"
     CDS             2738..2941
                     /locus_tag="PLS35_12620"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HNI19557.1"
                     /translation="MSKTCEHCRQPFECVGYQCWCGKIGITEAQMDWIAARYQDCLCP
                     ACLQQVADGKLGPGVPPQADQAR"
     gene            3006..3602
                     /locus_tag="PLS35_12625"
     CDS             3006..3602
                     /locus_tag="PLS35_12625"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNI19558.1"
                     /translation="MRITKVYTRTGDAGQTRLAGGQQVWKDCVRVEAYGTVDELNASV
                     GLVRAMNAEGGSASPASAQLETDLRWIQNKLFDVGSLLATAPGQSFPNMPKVTEADVT
                     RLEQMIDRCQEDLAPLKEFILPGGGKVSATLHQARTICRRAERDCIRLSREEDVSAEL
                     NKYLNRLSDALFVMARWVAKTQGEPEFLWQRESDAPIK"
     gene            3586..4143
                     /gene="cobU"
                     /locus_tag="PLS35_12630"
     CDS             3586..4143
                     /gene="cobU"
                     /locus_tag="PLS35_12630"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNI19559.1"
                     /translation="MPRSSKSTTRPGRAGSRLILVLGGASSGKSQAALERAGSQAPKA
                     FVATGQGLDKEMQARIARHRATRSAEWVTAEVPRDLAGWFRSDGSGYRTIVVDCLTLW
                     LSNVCGRRLAGADMSARVDELVHAIRQSPARVVLVSNELGFGLVPTSRSARAFRDVAG
                     RVNQQIAASADEVYFVISGQSLRLK"
     gene            4153..5208
                     /gene="cobT"
                     /locus_tag="PLS35_12635"
     CDS             4153..5208
                     /gene="cobT"
                     /locus_tag="PLS35_12635"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNI19560.1"
                     /translation="MMTLDDAMGLIQPVNGDSAANAQRRLDRLTKPLGSLGRLEELAV
                     HYARITGDVKPNVPRGVVFTFAADHGVAMEGVSAYPSEVTPQMVLNFLRGGAGVNVLA
                     RHTGVEVRVVDIGVAYDFGVVPGLIQKKIMRGTNNLLREAAMTRAQATEALQVGIDLA
                     TEAAQEGVGLIGTGEMGIGNTTPSAGITAVMTGRPVAEVTDRGTGIDEAGHARKVSVI
                     EQALNRHRPDKADALDVLAKVGGLEIAGLAGLMLGAAAARVPVVLDGFIAGAAALIAV
                     ALQPLCRDYLIASHRSVERGHQATLECLALKPLLDLDLRLGEGTGACLGMGLVQASIK
                     ILTEMATFDEAGVTERD"
     gene            5201..5974
                     /gene="cobS"
                     /locus_tag="PLS35_12640"
     CDS             5201..5974
                     /gene="cobS"
                     /locus_tag="PLS35_12640"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNI19561.1"
                     /translation="MTEIAAMTRPFVFAWHFLTAIPISRTHHAPTAQELASSMAWYST
                     VGLAIGGLVALADVGLRSWMAAEVGNALLIVLLVLLTRGLHQDGLADTVDGLAGGRTV
                     EDRLRIMRDPRVGAFGATALSLSLLLRYAGLLALPAPLRLPVLLCMPALGRWAMVSLA
                     WASPSARRDGGLAASFLTHLSWQQVLLSSLVLAVALTMALGGTVAVATMGMGVLLLVL
                     IRQRCLTLLGGVTGDVLGATNEVVEMLFLLLIPALVVAA"
     gene            5971..6927
                     /gene="cbiB"
                     /locus_tag="PLS35_12645"
     CDS             5971..6927
                     /gene="cbiB"
                     /locus_tag="PLS35_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HNI19562.1"
                     /translation="MTGVELAVAAALDVAAGDPRWLPHPVKGMGAVIAWVDDRVRRLC
                     RSEQALQIAGGCLALGLPIAVYAAATWVIAQATAIAPLLGQVVGVGLAYTTLAGRDLF
                     DHVQAVLRELAAGNLAGARQAVAMIVGRDSATLTESGVARATVETIAESTSDGIIAPL
                     VYLSLGGAPLALAYKAVNTLDSMIGHLDARYEHFGWASARLDDVLNWVPARLAGGFIA
                     LAAGLSTGQWHRIHESWYMLHRDGDKHASPNSGQPEAAMAGALGVQLGGTNYYDGVAH
                     EAPTIGDGTADITPEHIRQASRIMIVTYGLGLFFALLSLWVS"
     assembly_gap    6976..7075
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7076..7855
                     /locus_tag="PLS35_12650"
     CDS             <7076..7855
                     /locus_tag="PLS35_12650"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNI19563.1"
                     /translation="AAMARVGGQISSIMATRDDGYRPPLEQALDNLDKFRNKGNSMPP
                     VDAVMLCNPNSPTGQACDAVMVRKLARRAARLGQWCLVDETFAEYCEQVSILPEDLPA
                     RTLVLRSFTKFYGLPGLRVGYAVGNPKVIKQVSAHQPPWSVNMLAQRAAQLALQDRRH
                     SRRSLRFIEQERSRLVQELRRLPGCSTFPSAANFLLVELPARLKATRVVSALRRQGIL
                     VRDCSQVPGLNDRSIRLAVRSRADNDRLLNSLSKIIGNSRV"
     gene            7852..8586
                     /locus_tag="PLS35_12655"
     CDS             7852..8586
                     /locus_tag="PLS35_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HNI19564.1"
                     /translation="MTQRHANRISTRHWVAGDTLIVDLGVRMRVLSSAPRGGGLRTTR
                     FILNHQVPSHPPGTDTTGRTWEHPSHYLRRLAETHGVEPDCVGLMTAVPMTQLVTHRE
                     ERDGTWVEGFATVGVTNAVRAGEPVRQVQGEQIGATAGTINLIVVTNGCLAVPAMVGA
                     VQVATESKTGVLRDHAVPSWTGLAGATGTGTDAVVVASALRGHGPWHPYAGTHTEIGS
                     MIGRVVAECLLQGLALAAQWADQRRT"
     gene            8602..10155
                     /locus_tag="PLS35_12660"
     CDS             8602..10155
                     /locus_tag="PLS35_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HNI19565.1"
                     /translation="MPHPSRAKAIAILGTGSDVGKSMVTAGLCRLLHRAGIRVAPFKA
                     QNMSLNSFATPEGGEIGRAQALQAEACGLAPHVDMNPILLKPESNARSQVVVQGKVFA
                     TLDARDYFTRGQDSRLLRAVRESYERLAAQYEVIVIEGAGSAAEVNLRDRDLVNWTTV
                     ELADARVVLVGDIDRGGVFAQIVGTLDLIAAEERARVCGIVINKFRGDRTLFDEGVRF
                     LTARTGIPILGVLPYLRDLALDQEDSLDVGARAQIPFTAERVNVAVLLLPHMSNFTDF
                     NALAQETDVALRYVATPEAALGADVMILPGSKTTLSDLAYLRERGFQSVLQDHVRRGG
                     ELVGVCGGYQMLGQSIADPDGVETGGHDKGLELLPVTTVLARDKITEQVEGQALHIEG
                     STTLTVRGYLIHVGRTDRAGLRPSFQLVRRKTSLDGSTAIVRDEECLDGAVGHDGLVW
                     GTYVHGLFDQPDFRRAWLNRLRSRKGLPAVSLEESQRVNDTRLNAMDRWADHVDRHLD
                     LAPVLSWLR"
     gene            10565..11299
                     /locus_tag="PLS35_12665"
     CDS             10565..11299
                     /locus_tag="PLS35_12665"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNI19566.1"
                     /translation="MANHFFHRIAMAVLAAACVGGPVAASANTEVETAELLIKLLQVG
                     RGVLSENQATINDASKGDKGFTGDYVANQVIDRFRKATKIDLSRPATVPQAPLFLAMV
                     ESEKEVIDEAQPVINKQGVGFKGIIPAVFARKSGERFYRKTGIRVKLTSIDYRFPGNR
                     PDDFESEVLRIFSDSRHPKGQPYSKATMLDGRPVLRMMDPEYAATTCLSCHGNPKGER
                     DVTGAKKEGWHEGDLAGAISIVIPMK"
     gene            11365..11727
                     /locus_tag="PLS35_12670"
     CDS             11365..11727
                     /locus_tag="PLS35_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19567.1"
                     /translation="MSKIVVVDDSYAELQLIEGYLKSANHTVVSYPNTDKLEDKLALD
                     KPDLIVLDVVMPGRNGFQACRDLKSDDRFKGIPIVLCTSKGQESDKFWGQQQGANGYV
                     VKPFKAEDLVAAVKRALN"
BASE COUNT         2263 a         3110 c         3838 g         2422 t
ORIGIN      
        1 aaaccgatac gaaattgcgc gtcggctatt cgaccggctt ccgggcaccc agcatgaatg
       61 agctgtactt tccgaacttc gggaatccca atctgggacc ggagaagaac caaagtatgg
      121 acgcggggat cgatcagtat tttttttcga aacagttgaa gctgagcgga ggcttcttct
      181 ggaatcatta tcgcaatctc atcgtcacga cctttgaccc ggggttttgc gcccccttca
      241 gcacgtttgg attctgtccg cagcaactgg gggaggcatc gaccaagggg tgggaggccg
      301 ggctcaccta tacctattcc agcgatcggc cgtttctgaa aggcgtggtg gttcaggcgc
      361 agtacacgaa tacgatcacg cgcgatctgg atacgcaaac gcgtttgcca cgctggccga
      421 ccgaccagtg gagcgcgtcc gtgagctatc aaccgatcga ccccttgtgg atcacggtga
      481 tcgggcgcta tgtcgggtcg cgattcaata caactggaga tcggcaagcc atgccggcgt
      541 tcgacgtgtg gtccctcgcc gtgacctacg atgtcacgaa gcagatgcaa gcctatctgc
      601 gagcggagaa cctgttcaat gagaagtatg aagaaatcgc cagtgccggg acgccgatcc
      661 ggtcgatttt cggaggtctt cgcgtgacgt tcggcggtaa atcgtaaatg gtccatgagc
      721 atcgtctggt gatgccacgc cctcgtctgg tcattgccgg cacccacagc ggtgtcggca
      781 aaaccaccgt cacccttgcg ctgatggccg cgttgaaggc cagagggctg gtggtgcaac
      841 cgttcaaggc cgggccggat tttatcgatc ccggacatca tcagctggtg accggtcggc
      901 cctcgcgcaa tctggacggg tggatgttga atgaggcggt caatcggtcg atcttttcgc
      961 aggcgtcggc cgatgcggat ctgtccatca tcgaagggat gatgggcctc tttgacggga
     1021 gttccccgat acacgagcag ggcagcacgg cagaattggc caaacagttg catgcgccgg
     1081 tgttgttggt gattgatggc agcgccatgg cgcgctcggc ggcggcgatg gcagccggat
     1141 atgcgcgatt cgatccgggc ctttctgtgc agggcgtgtt gttcaatcga gtgcgaagcg
     1201 acgggcacta ccgcttgctc caggaggcgg tggaagcgga gacggggctc gccgtcgtcg
     1261 ggtaccttcg gcctgatgcc tcggttacga tccctgaccg gcatctcggt ctcaggaccg
     1321 ccattgaggc agggtcatgt gacctctacg accgactggc gcagtcggct gccgagacga
     1381 ttgatctggc tcgagtggag gcgatcgccc gatcggctga aggatggttt gaagaagatc
     1441 cggtcagtcc acctgcggca ggaccgaaag aaacggtccg tgttggtgtg gcctatgacg
     1501 gtggattttg cttttactat caggacaacc tggaattact cgcagcggcc ggtgctgaga
     1561 tcgtacaatt ctcgcccttg catgatgcag ctctgccgga tgtggatctg ttgtatttcg
     1621 gcggcggcta tcctgagctc tatggtgagg cattggctgc caacgtgagc atgcgggccg
     1681 cggtgaaggc gttcagcgac cgcggtggag tgatctatgc ggaatgcggc ggcttgatgt
     1741 atttgacgca agccattcgt gatggagacg gtcatcgcta tgagatggtc ggtgtctttc
     1801 ctgccgaggc ggtcatgcgg aggtcgggga tgacgttggg gtaccggatc gtcgaaacga
     1861 tgcggccctc tctgctgggc ccggccggca cgtcagtgcg cgggcatgag ttccattatt
     1921 ccctcctcga gccgaagggc gagttggcgt atgcctgtgt gctggcagat gcagcgggca
     1981 agccggttgg tcaggatggc gtgatggtca gaaacacctt ggcattgtat agtcaccagc
     2041 atttccggag ccgtcccgaa gtggtggtca atctgctcca gtctgtgcga caagagaaag
     2101 ctgcgcgcca agcatgagga agcagtgaga tgactgatcg ggatgaacac accgccaaaa
     2161 tgcagcgcct gaaagggtcg gtcgatcgtc gtatcgaagc agctcaggag gaaaagggac
     2221 tgctgatggt ctacaccggc gcgggcaagg gcaaaacaac tgccgcgctc ggtatggcga
     2281 ttcgctgcct cggtcatggc atgaaggtgg cgattgtgca attcatcaag ggggctatcg
     2341 atacggcgga ggaacgtatt ctcaggtcat tcggcgatcg tgtgacgttt ctgcgcatgg
     2401 gcgaagggta cacgtgggaa acgcaagatc gcgaacgcga tacgagattc gctcaggagg
     2461 cctggaacaa agcctgcgag ttcatgaccg atccctccta tgcgatggtg attctcgacg
     2521 agttcaacat tgccctgcac caccactatg tggagctggc ggaagtgctg ccgcgattgc
     2581 aggctcgtcc gctgatgcaa catgtggtca ttaccggtcg aggggctccg gctgagctga
     2641 tcgacgcggc tgatttggtg accgagatga aacaggtcaa acatcccttt cgacaaggca
     2701 ttaaagcgca ggcaggggtg gagttttgag aggcccggtg agcaagacgt gcgagcattg
     2761 ccgtcagccg ttcgagtgtg tcgggtacca atgttggtgc gggaagatcg gcattaccga
     2821 agcccaaatg gattggatcg ccgcccgtta tcaggattgt ctctgtcccg cgtgcctgca
     2881 acaggtggcc gatggaaagc ttggaccggg agtgccgcct caggcagatc aagcgaggta
     2941 acgtgtcgat accggggttc ggcctcgtga gtcgagtggc gaatccggag tgaaaggatc
     3001 agagcatgcg aattaccaaa gtctatacac gaaccggcga cgcgggccaa acaaggcttg
     3061 ccggagggca gcaggtctgg aaagactgtg tgcgcgttga agcctatggc accgtcgatg
     3121 agctgaatgc ctctgtggga ttggtgcggg cgatgaatgc cgaaggcggg agcgcctctc
     3181 ccgcctccgc gcaactcgag acggacttgc gctggatcca aaataaactg ttcgatgtcg
     3241 ggagtctgct cgccaccgcg ccgggccaga gctttccgaa tatgccgaaa gtcaccgagg
     3301 ctgacgtgac caggctcgag cagatgatcg atcgctgtca ggaagacctg gcaccgctca
     3361 aggaatttat tctgcctggc ggggggaaag tctcggcgac gctgcatcaa gcgcgcacga
     3421 tctgcaggcg ggccgagcgc gattgcatcc ggctcagccg tgaggaagac gtgtccgccg
     3481 agctcaataa gtacctgaac cggttgagcg atgccttgtt cgtcatggca cgatgggtag
     3541 ccaaaaccca aggtgagccg gagtttttgt ggcagcgtga atctgatgcc ccgatcaagt
     3601 aaatcgacga ccaggcccgg gcgtgcaggg tcccgcctca ttctggtgct ggggggcgcc
     3661 tcctcaggga aaagccaggc ggcattggaa cgggcaggca gccaagcgcc aaaggccttt
     3721 gtcgcgaccg ggcagggatt ggataaggaa atgcaggcgc gtattgcgcg gcaccgggcg
     3781 acacgttccg ccgagtgggt gacggctgag gtgccacgag atctggcggg ctggtttcga
     3841 tcggacggat cgggctatcg cacaatcgtc gtcgattgcc tgacgctctg gctcagcaat
     3901 gtctgcggcc gccgtcttgc cggcgcagac atgagtgctc gagtcgatga acttgtgcac
     3961 gccattcgac agagtcccgc gcgtgttgtg ctggtcagca acgaactggg attcggcttg
     4021 gtgccgacaa gtcgcagcgc gcgcgccttt cgtgatgtcg ccggacgggt caatcagcag
     4081 atcgccgcct cagctgacga ggtctacttt gttatcagcg gtcagtcgct caggctcaaa
     4141 taaataggag agatgatgac actcgatgac gcgatgggcc tgattcaacc agtgaatggt
     4201 gacagcgcgg cgaacgctca gcgacgcctt gatcgactca ccaagccgtt gggcagtctg
     4261 gggcggctgg aggagttggc cgtgcactac gccaggatca cgggcgatgt gaagccgaat
     4321 gtgccgcgtg gcgtcgtgtt tacctttgca gctgatcacg gcgtggcgat ggaaggtgtg
     4381 agcgcctatc ccagtgaggt cacaccccag atggttctga atttcctgcg gggcggtgcc
     4441 ggggtgaatg tcctggcgcg acatacggga gtggaagttc gggtcgtgga tatcggtgtc
     4501 gcctacgact tcggggtcgt gcccggactc attcagaaaa agatcatgcg ggggacgaac
     4561 aatctcctgc gtgaagcggc catgaccagg gcgcaggcga cggaggcatt gcaggtggga
     4621 atcgatctgg cgacggaggc tgcgcaggag ggggtcggcc ttatcgggac cggggaaatg
     4681 gggatcggca acacgactcc aagcgcgggc atcacggccg tcatgaccgg tcgaccggtt
     4741 gctgaagtga cggatcgtgg aaccggcatc gacgaagccg gtcacgcccg caaggtttcg
     4801 gtcatcgagc aggcgctgaa tcggcatcgt cctgataaag ctgatgcgct tgatgtgctg
     4861 gccaaggtgg gcggactgga aatcgccggg ctggccgggc tcatgctagg cgcggcggca
     4921 gcacgcgtgc cggtggtgtt ggatgggttt attgccggtg ccgccgcctt gatagccgtc
     4981 gcgttgcagc cgttgtgtcg tgactacctg attgcgtcgc accggtcggt cgaacggggg
     5041 catcaggcga ctctggaatg tctggctctg aagccgctcc tcgatctgga tctccggctg
     5101 ggggagggga cgggggcttg cctggggatg gggctcgtcc aggcctcaat caaaattctc
     5161 accgagatgg cgaccttcga tgaagcgggt gtgacggaac gtgactgaaa ttgccgccat
     5221 gacaagaccg tttgtgttcg cctggcattt cttgacggcg atcccgatca gccgaactca
     5281 tcacgcgccg acggctcagg agttggcgtc atcgatggcc tggtattcca ccgtcggtct
     5341 cgcgatcggc ggcctggtgg cgttggccga tgttggcttg cgctcgtgga tggcagcgga
     5401 ggtcggcaat gcgctgttga ttgtgctgct cgtgctgctc acgcgcgggt tgcatcagga
     5461 tggcctggcg gacacggtcg atggactggc cggcggccga accgttgagg atcgtttgcg
     5521 aatcatgcgc gatccgcgag tcggtgcgtt cggcgcgacc gccttgagtc tttccctgtt
     5581 gctacgatat gcagggttac tggcgctgcc tgcaccgctg cgactgccgg tgctgctgtg
     5641 catgccggca ctgggtcgct gggcgatggt tagtttggca tgggcgtcgc cctcagcaag
     5701 gcgtgatggg ggattggctg cctcgtttct cacgcacctg tcctggcaac aggttcttct
     5761 ttcgagcctc gtattggctg tggcgttgac gatggccttg ggcggaactg tggcggtcgc
     5821 gaccatgggc atgggtgtcc tgttgctggt cctgatcagg cagcggtgtc tgaccttgct
     5881 tggcggcgtg acgggcgatg tgctcggagc cacgaacgaa gtggtcgaga tgctctttct
     5941 cttattgatt ccggcgttgg tggtagccgc atgactggtg tggagttagc agtcgccgcg
     6001 gcgctggatg tcgcggcggg agatcctcgc tggttgcctc atccggtgaa gggcatggga
     6061 gccgtcatcg cctgggtgga tgatcgtgtt cgccgccttt gccggagtga gcaggccctc
     6121 cagattgcgg ggggctgcct ggcgctgggc ttaccgatcg cggtgtatgc cgcggcaact
     6181 tgggtgattg cgcaagccac ggcgatcgct cctctgctcg ggcaggtggt cggcgttgga
     6241 ttggcatata cgacgctggc ggggcgggac ttgttcgacc acgttcaggc ggtgctccgt
     6301 gaattggctg ccggcaatct ggccggtgcc cgccaagctg tggcgatgat cgtcggccgt
     6361 gatagtgcca cgctgacaga gtccggcgtg gcccgggcca cggtggaaac catcgcggaa
     6421 agtacatccg acgggatcat tgcacccctg gtctatctgt ccctgggcgg ggcgccgctg
     6481 gcgctggcct ataaggcggt caatacgctc gactccatga tcggtcacct ggatgcccgg
     6541 tatgagcatt tcggttgggc ctccgcccga ctcgacgatg tgctgaattg ggtgccggca
     6601 cgattggccg gcggcttcat cgcactggct gcaggcctat caacaggcca atggcatcgg
     6661 attcatgaaa gttggtacat gctccatcga gacggggata aacacgccag tccgaacagt
     6721 ggccagccgg aagcagccat ggccggagcc ttgggcgtgc aactgggtgg aacaaattat
     6781 tatgacggtg tggcgcacga agctccgacc atcggtgacg gaaccgccga catcactccg
     6841 gagcacatcc gccaggcgtc tcgcatcatg atcgtgacct atggtctggg tctgttcttc
     6901 gccctgcttt ccctatgggt atcatgaacc gccaggctca tgggggggat gtctatgcgg
     6961 cggctcgtga attgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcagc
     7081 catggctcgc gtcggtgggc agatcagcag catcatggcc acacgtgacg acggataccg
     7141 gcctccgctt gagcaggcgc tggacaatct ggacaagttc cggaacaagg gaaacagcat
     7201 gccgcctgtt gatgcggtga tgctgtgtaa ccccaacagt ccgaccggcc aggcctgcga
     7261 tgccgtcatg gtcagaaaat tggcccgccg ggcggctcgt cttggacaat ggtgtctcgt
     7321 ggatgaaacc tttgccgaat attgcgagca ggtctcaatt ctgccggaag acctgcctgc
     7381 gcggaccctc gtgttacgca gcttcaccaa gttttacgga ttgcccggtc tccgtgtggg
     7441 ctacgcggtg gggaatccca aggtcataaa acaagtgagc gcgcatcagc ctccctggtc
     7501 ggtgaatatg ctggctcagc gagcggctca actcgcactg caggacaggc ggcacagccg
     7561 gcgaagcctc cgattcattg aacaagagcg atcgcggctt gtgcaggagt tgaggcgatt
     7621 acccggctgt tcgacgttcc cgtccgcggc caatttccta ctcgtggagc tgccggcaag
     7681 actgaaggcc acgcgcgtgg tatcggccct tcggcggcaa ggaattctgg ttcgagactg
     7741 ttcgcaggtg ccgggactga atgatcgttc gatacgcctg gccgttcgct cgcgcgccga
     7801 caatgatcgc ctcctcaact ctttatccaa aatcatcggg aatagtcgcg tatgacccag
     7861 cgtcatgcaa accgcatctc gacgaggcat tgggtcgccg gcgatacgtt gatcgtggat
     7921 ctcggcgtac gcatgcgcgt cctctcctcg gctccccggg gcggagggct tcgcacgact
     7981 cgttttatcc tgaatcatca ggtgccctca catccgcccg ggacggacac tacagggcga
     8041 acctgggaac atccatccca ttacctcagg cggcttgcgg agacgcacgg cgtggaaccg
     8101 gactgtgtcg gactcatgac ggcggttccc atgacgcagc ttgtgaccca tcgagaggag
     8161 cgagatggaa cgtgggtaga ggggtttgcc accgtggggg tgacgaatgc cgtgcgggcc
     8221 ggggagccag ttcgtcaggt gcaaggcgaa cagatcggtg cgacagcggg aacgattaac
     8281 ctcatcgtgg tgacgaacgg ctgtcttgcg gttcctgcca tggtcggcgc ggtgcaggtg
     8341 gcgacggaga gcaaaacggg cgtgttgcgc gatcatgccg ttcccagctg gaccggcctt
     8401 gccggtgcga ccgggacggg aacggatgcg gtggtggtcg cctcagctct tcgagggcat
     8461 ggcccctggc atccgtatgc ggggacccat acagagatcg ggtcgatgat cggtcgcgtc
     8521 gtggctgagt gtcttctcca aggcttggcc ctcgcagcac agtgggccga tcagcgtcgg
     8581 acctaacctc cgagctttct catgcctcat ccttctcgtg ccaaagccat tgcgattctc
     8641 gggaccggct ccgatgtcgg gaagagcatg gtgacggcag ggctctgccg gctgctgcat
     8701 cgcgccggaa ttcgcgtggc cccctttaaa gcgcaaaaca tgtcgctgaa ctcattcgcc
     8761 acaccggagg gtggcgagat cgggcgtgcc caggccttgc aagccgaggc ctgcggcctt
     8821 gctccccatg tcgacatgaa tcccatcctg ctcaagccgg aatcgaacgc cagatcacaa
     8881 gtggtggtgc aggggaaagt ctttgcgaca ttggatgcac gcgattattt cacgcgcggg
     8941 caggattccc gcttgttgcg cgcggtccgc gagagttacg aacggctcgc agcgcaatac
     9001 gaggtgatcg tgatcgaagg agccggtagt gccgccgaag tgaatttgcg cgaccgtgat
     9061 ctcgtcaact ggactacagt ggaactggcg gatgcccggg tggtgttggt gggtgatatc
     9121 gaccgcggcg gcgtcttcgc ccaaatcgtc gggacgttgg acctgatcgc agcggaggag
     9181 cgcgcccgtg tctgcgggat cgtgatcaac aaatttcgag gggacaggac actcttcgac
     9241 gagggtgtcc gttttctcac cgcacgcaca ggaataccca tactcggagt gttgccgtac
     9301 cttcgcgatc tcgcactgga ccaggaggac agtcttgacg taggtgcccg ggcgcaaatt
     9361 cctttcacgg cggaacgtgt caatgttgcc gtgctgctcc tgccgcacat gagtaacttc
     9421 accgatttta atgcgctcgc gcaagaaacg gatgtcgcgc tgcgatatgt cgcaacgcct
     9481 gaagcggcac taggcgcaga cgtcatgatc ctgcccggtt cgaagaccac actgtcggac
     9541 cttgcgtatc tgcgcgagag gggatttcag agtgtgttgc aggaccatgt gcggcgtgga
     9601 ggggaattgg tgggcgtgtg cggcggctat caaatgcttg gacaatcaat tgccgatccc
     9661 gatggagttg agaccggcgg tcacgataag gggttagaat tgttgccggt caccaccgtg
     9721 ttggcgcgag acaaaattac ggagcaggtc gaggggcagg ctctgcacat tgagggatca
     9781 acgactttga ctgtccgagg atatctcatc cacgtgggca gaacggatcg cgcggggctt
     9841 cgtccctcct ttcagctcgt gcgaagaaag accagtctgg acggcagcac ggcaatcgtt
     9901 cgcgatgagg aatgtctgga tggtgcggtg gggcatgacg ggttggtatg gggaacctat
     9961 gtccatggtc tcttcgatca accggatttt cgccgtgctt ggctgaatcg gctgcgcagc
    10021 cgcaaggggt taccggcggt ttctctggaa gagtctcaac gcgtgaacga cacacgtctg
    10081 aacgcgatgg atcgctgggc tgatcatgtg gatcggcacc ttgatctcgc tccggttttg
    10141 tcatggctcc gatagtctga tcagatttcg tctgacagtg tctagccatt ccagctccac
    10201 gtcattttga cacttctccc gtcaagcgac caaactttgg cgctccgctc gaaatgttga
    10261 acaattgaga accctgctca gacgcactga tcatagtggg tatagttgat cgtccacatc
    10321 taatcatacg caaaatcgcc gacttcattt cattccactc ctcccctggt cgaagatact
    10381 aagtaattgt tttgtttggc accgaacgtg cacgaggcag gccggggctg caggggctga
    10441 cgtacctgtc acgaggccaa aaattgtcaa tcgctaattt tgcgctatct ttgacagagg
    10501 tttggcgcag ttagtacgag cggattcctc acgagcgaca tcgagatcgg caggaggagg
    10561 gggcatggca aatcattttt ttcacagaat tgcgatggct gtcctggcgg cggcctgtgt
    10621 gggcgggccc gttgccgcgt cggctaatac cgaggtggaa acggctgaat tattaattaa
    10681 attgctgcaa gtcggccgcg gggttctgtc ggaaaaccag gcgacgatca atgatgccag
    10741 taagggcgat aagggattta ccggggacta tgtcgcgaac caggtcatcg accggtttcg
    10801 taaggcgacg aagatcgacc tgagccgccc ggccaccgtg ccgcaagcgc cgctgttcct
    10861 ggctatggtc gaatcggaga aagaagtcat cgatgaggcg cagcccgtaa tcaataagca
    10921 gggcgtgggc ttcaaaggca tcattcccgc cgtgttcgca cgcaagtccg gcgagcggtt
    10981 ctatcgcaag accggcattc gcgtgaaact gacgagcatc gactatcggt ttccaggcaa
    11041 ccgtccggat gattttgaat ccgaagtctt acgcattttt tcagactcgc gccatcctaa
    11101 gggacagccg tacagcaagg ccaccatgct ggatggaaga ccggtgctgc gcatgatgga
    11161 tccggagtat gccgcgacca catgtctcag ctgccatggg aatccgaaag gcgagcgtga
    11221 tgtcacgggc gcgaagaaag aaggctggca tgagggcgat ctggccggcg ccatcagcat
    11281 tgtcattcct atgaagtagg cacgacggcg cagtccagac aggcgtcgaa aacttttgaa
    11341 tggttgtgaa ggggggatgg attcatgagc aaaatcgttg tggtcgatga ttcgtacgcc
    11401 gaactgcagt tgatcgaagg gtatctcaag tctgccaatc ataccgtggt gtcctacccc
    11461 aataccgaca agctcgaaga taagttggcg ttggataagc cggatctgat cgtgttggac
    11521 gtggtgatgc ccgggcgcaa cggattccag gcctgtcgcg acttgaagag tgatgaccgg
    11581 ttcaagggta ttccgatcgt gctgtgcacc tccaagggac aggaaagcga caagttttgg
    11641 gggcagcaac agggggcgaa cggctacgta gtgaagccgt tcaaggccga agatttagtt
    11701 gcagctgtga agcgggcact gaactaactc atg
//
LOCUS       DAOQEW010000116        11642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1644_length_11642_cov_8.215155, whole genome shotgun sequence.
ACCESSION   DAOQEW010000116 DAOQEW010000000
VERSION     DAOQEW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_11642_cov_8.215155"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..3736)
                     /locus_tag="PLS35_12675"
     CDS             complement(305..3736)
                     /locus_tag="PLS35_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19568.1"
                     /translation="MAELYETAVTLLEEQPLPGRSRIIAHCVREIANALPALLAGVER
                     TRLEYDKRLDKIALARHRLTRLPIQAPRSMGGTSISDPQDALTQEFFNQIEELIKEHK
                     ATRLKRDTIAERLFQANRPENRHLGDALRPVLRRWTDLKDWFVGHAHDNGKTDAQCDW
                     SDIKRRFVLFERTVLTLGQGFFATIADLDELIRTSTPADVHLVVTQLGHIEHYKYFFE
                     HLDDPAWIPALRAEGIFNAPPSVEPNAIPRWAASEFLVRVIDRGDPTEGMKALSQIAD
                     ALTQQSLVNPLVIRDVVDVLRKVPAEHSVSFMPRLKQWAHHDRGFLFWRQLGKLVTHL
                     AEGGKPHEALTLLTPLLAIEGVRADDDFEYSYKPVKPRISMYEYIQIVDEHLRPVLER
                     RGEELFDVLCNILQKAAKLSRRSHSSEEWDDNSRVWQPDLSIQDEDDRDDVRSVLVVR
                     IRDAAETLLNDGRVDLGPLVEDLEARRWLIFRRLALYCLVTTASAPRDLIAPRLTHEA
                     SFRDRNLGKEYDSLLHIGFTRLTPDEQQTILSWVEAGPENIEQLIRNYETSTGRRATQ
                     EDIEKHHRSWQWKRLWPCHTALPATWRERYATLVEEFGEIEAPLPTTREARDVLEGER
                     APRPEQDLRTLTPAALREYLLAWTPERTTFPYPTRSGLAEVLTSLVTADPVAYANATE
                     EWLHLDPTFLHGIIRGFTKALQVDHPFSWAPVLSLCRWILAEPRTIPGRSVDRWEADL
                     DWGGTRWWIVELLRVGFQDETHGIPIDLRDVAWQLLETLTMDPDPEADEDEEDFGRPS
                     QSMNRAISSVRGRAIEALMFYPGWIKRQTGDAGPPRLPAEARAVLDRHLDLTEDPSLA
                     IRSLYGRWLPWILVFARDWGTASIPRIFPEYSQRYWLAAWEGFISFNNPYPEIFEPLQ
                     PVYAQAIAQLGSPSAEADDTRDQRDERLAGHLMTYYWRGHYPIEDETGLPYQFFARAS
                     DQVRAEAIEFIGRSLDRTDDPVESVVLDRLQRLWAQRFDGARQDPATHQAELRAFGWC
                     FGSGKFEPRWAVQNLLDVLRLTQSIDPDFQVLERLPDHAGAFPLEVLECAKRLIEGQT
                     SHVELSSWGEDLRRIFSQTRNHPAPEVRQASDDVIEVLGRFGHLGYADLLSRESG"
     gene            complement(4149..5042)
                     /locus_tag="PLS35_12680"
     CDS             complement(4149..5042)
                     /locus_tag="PLS35_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19569.1"
                     /translation="MGQAGRCVGSMVALCGLLLAVACGDASVDAYCEANGRGNQDTLD
                     LLTHVLEQKRNQTGIYLARARCYYFLGSYAQALQDASEVIRRLPNKADAYLIRAKAGK
                     MLGQIPQALDDYSTSIRLRPSAEAHYGRGLTYVREYQGKDREALDDFTAAIRFDPKHV
                     GAYKARAEIYSRHEQFQNALQDSEIVLKLDPATPNAYCNVGFAHYAVGHDAEGRKLLN
                     ICYQKDPDPQTRGYYETEVRKVLAARQPRRNSDGGYQSDVRERTPYDREMERQQNVYE
                     SLRNSGFNDRAEACRTDGSKC"
     gene            complement(5191..5976)
                     /locus_tag="PLS35_12685"
     CDS             complement(5191..5976)
                     /locus_tag="PLS35_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNI19570.1"
                     /translation="MRSAGTRIVRWLLCACLAGVAQPALALDHDNLDPNRPIGMEDAY
                     AIPKGEIGLEGGVRFNDRREGRTQVTFQPQIIYGAFANTQIEIQGDLFTDPHSIVGAN
                     KSGDLHLGLLYNFNTETLILPAMAVRVEADLPTGVNSKGVDTQVTGILTRSFGRLRVH
                     LNGGYTVLGSPQGQERPGTYRAVAAVSYPLGYPGSFRDTLIASLYTRQSDLHGERNHT
                     GVEIGLRHQLTSRVVLDGGLGTEFLGPANRAALLGTVGVSLGF"
     gene            complement(5973..6887)
                     /locus_tag="PLS35_12690"
     CDS             complement(5973..6887)
                     /locus_tag="PLS35_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19571.1"
                     /translation="MAIATVAFALAGCNQFEPRDKRFYYRALWNFSLREDLAELDSEF
                     NGVDFGHSNLYENLLLTGGQDVAAIEDRARKETLAFIATRPRLNPNEEAIAPTYMKLA
                     WRAQNTFDEAHALHRATYDIAVSDEPDKDRAIRNVLAYYKESAYAITSKQLDHRRLDS
                     FPYSKAFRQRFPLFNATIWSYHYLQVAVYDPLLAARDLTAKTQAVRPILTTYRRYLEQ
                     PPVEWTFMPLTAELSPEFAARYPDVAHIFDNLHMLHDNISDILTSERLPTWEAKRAEI
                     YRIVNTYYLATADATNPMIVQTAGEHHH"
     gene            complement(6952..7533)
                     /locus_tag="PLS35_12695"
     CDS             complement(6952..7533)
                     /locus_tag="PLS35_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HNI19572.1"
                     /translation="MRHHHSAAAAVPRQAESDSQVHHHDHERPQPHGHAPHQAHSAER
                     HPTPSLDRTALMATLHCLSGCTIGELLGMAIGTALGWGNWSTIALAIALAFFFGYAMT
                     VYPLRRAGMAWGTALGLALASDTLSMTTMELADNAIMMVIPGAMDAGIFDLLFWGSLG
                     FSLIVAGIAAFPVNRWLIARGKGHALVHGHHCH"
     gene            7818..8324
                     /locus_tag="PLS35_12700"
     CDS             7818..8324
                     /locus_tag="PLS35_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19573.1"
                     /translation="MPSGIHRTLYTLFFLLLAPLTTQAADLPNTSLTPLRFLVGEWKG
                     TDADGKAHKSAFTLSSGGTTLTEVLTPPDSPPMTTMYYSDGDQLMLTHYCSLDNQPRM
                     RAAAVKDGDKTITFSFVDATNLKNPTDVHMHQLAIDVKDHDHFTQTWTLSKAGKDVPK
                     VFTFERVK"
     gene            8358..9215
                     /locus_tag="PLS35_12705"
     CDS             8358..9215
                     /locus_tag="PLS35_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI19574.1"
                     /translation="MPSQTPAAVIEAQREDWNRVAAGWDKWDQFFNRTMAFINHRLVA
                     DARIRPGLRVLDLGSGTGYPAILAGEVVGADGTVVGMDLAESMLAVATRKAKTLGLHQ
                     VSFRTGDVTSLPFDSGSIDSIISRFCLMFLPEIPKATAEIARVLKPGGYVAAAVWSSP
                     DNNPFIRIPMDVIKTILPLPPPDPEAPGIFRLARPGDLAGMFERAGLTVLDDEEFTAE
                     VTYTTAEEFFRGLMDIAAPIQNLFAKLTPAQQTEAERGIIKTVNEYKRAEGVSLPIAV
                     RIVSARKPK"
     gene            complement(9283..9561)
                     /locus_tag="PLS35_12710"
     CDS             complement(9283..9561)
                     /locus_tag="PLS35_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19575.1"
                     /translation="MRTFMTVAAVVVCFSTALVFSADASETKAKMEKAKGDMKGNVEE
                     MKGETKAMNEELKGNDTKAEVERAKGKMKGAKERAKGKMKQLKEKATD"
     gene            9761..10195
                     /locus_tag="PLS35_12715"
     CDS             9761..10195
                     /locus_tag="PLS35_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19576.1"
                     /translation="MRHLILSGLLGFVILTTSGCSYLFYPHAKEFTEKAKGGNTVETL
                     INLTNMMEASAKAGQQGKGKDQALDDLHNQLHAFNNTICCVDKNKQETPAYALAVTHN
                     KELWAIFKRVWKFKDDQPQRSQHLELFVTEVRELRESLQALK"
     gene            10361..10732
                     /locus_tag="PLS35_12720"
     CDS             10361..10732
                     /locus_tag="PLS35_12720"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNI19577.1"
                     /translation="MRPDSFKDFVVDQLGEVPQLMCKAMFGGHGLYQRETFFGIIHKG
                     RLYMKTNGMTQSAYTARGMQPFRPNGKQLLRHYYEVPVDILEDAEQLTDWALRAITPP
                     TAQLILPFHDNPSIKDVLTHM"
     gene            10732..11304
                     /locus_tag="PLS35_12725"
     CDS             10732..11304
                     /locus_tag="PLS35_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HNI19578.1"
                     /translation="MNQELTTPSRRLWTIGHSTRSSDEFLALLHVHDIQRLIDVRRHA
                     GSRRYPHFHSDALAQSLPMAGLSYEELPALGGRRTARADSPNSGWKNASFRGYADYMQ
                     TEEFDRALEELMAQGENDRTAIMCAEAVPWRCHRSLVADALVARGWDVTHILGGGQGK
                     PHQLTTFAMIQAGRVIYPAPSDQCSTLRMF"
     gene            11356..>11642
                     /locus_tag="PLS35_12730"
     CDS             11356..>11642
                     /locus_tag="PLS35_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HNI19579.1"
                     /translation="MWYRTGADFVLLLHVSFVLFVIAGGLLLLKWRGLAWIHLPAVAW
                     GVLVELTGWICPLTPLENHLRSLAGESSAEGDFVGRYLPSLLYPAALTREI"
BASE COUNT         2505 a         3403 c         3162 g         2572 t
ORIGIN      
        1 gacgttcttc gtgaccggag aattctttga cgacgagttc gccctcttcc caataaagaa
       61 catcggcctt gatcaccatg gcggcagtgg cctgggcccc ctctaacgct gatgcaggaa
      121 tgactgcgga aaagatgagg tagaacgcga gaagaagtgc catcacaaac cctcccacta
      181 tatttaattt atgggctaca gaggatgcct gtcaattaga gttcattgtt accgcagctt
      241 gatctcatta gaatattgcg aaattgatag gtcaccaagg acgattgcaa gggtccctct
      301 tcgtttagcc tgactcccga gaaaggagat ctgcatagcc gaggtgacca aatcttccaa
      361 gaacttcgat gacatcatcg gaggcctgcc ttacctcggg tgccgggtga ttcctggtct
      421 gggagaagat ccttcgaagg tcctcacccc aactggaaag ctcgacatga gacgtttgcc
      481 cttcaatcaa acgcttcgca cattcgagaa cctcgagtgg aaaagcaccg gcatggtcag
      541 gcaggcgctc cagaacctgg aaatctggat cgatactctg tgtcaaccgc aggacgtcca
      601 gaaggttttg gactgcccac cgtggctcaa attttcccga tccaaaacac cacccaaacg
      661 cacggagttc tgcctgatgc gttgctgggt cttgccttgc tccatcgaac ctctgagccc
      721 atagccgttg aaggcggtct aaaactaccg actccactgg atcatccgta cggtccaaac
      781 tcctacctat aaactcaatc gcctcggcgc gaacctgatc agacgcccga gcgaaaaatt
      841 ggtaaggaag gccggtctcg tcttcgatcg ggtagtgtcc acgccaatag tatgtcatga
      901 ggtgaccggc tagtctttca tctcgttggt cccgagtgtc atctgcctcg gccgatggac
      961 ttccaagctg cgcgatggcc tgcgcatata ccggctgaag cggttcaaat atttccgggt
     1021 atggattatt aaaagatatg aagccctccc atgcggctag ccagtacctt tggctgtact
     1081 cggggaatat ccgaggtatg ctggcagttc cccaatcccg ggcgaacacc agaatccacg
     1141 gaagccagcg gccatatagc gagcgaatag ccagtgatgg atcttctgtc agatccaaat
     1201 gcctatccaa cacagctcgc gcttctgctg gaagtcttgg aggtccagcg tctcctgtct
     1261 gccgttttat ccaccccgga tagaacatga gggcttcgat agctcgaccg cgcaccgaac
     1321 tgattgcgcg attcattgac tggcttggcc ttccaaaatc ctcttcatcc tcgtccgctt
     1381 ctggatctgg atccattgtt aatgtctcca acagttgcca agccacatca cgaaggtcga
     1441 tgggaatgcc atgcgtctca tcctgaaaac cgacacgaag gagttcaaca atccaccaac
     1501 gggttccccc ccaatccaaa tcggcttccc agcgatcaac actccttcca ggaattgtgc
     1561 gcggttcagc aaggatccac cgacacagag ataagacagg cgcccaggaa aagggatgat
     1621 ctacctgcag tgctttcgtg aagccacgaa taatgccgtg aaggaacgtc gggtctagat
     1681 gcagccattc ttccgttgca ttggcatacg caacagggtc agctgtaacg agagaagtta
     1741 aaacctccgc caatcctgag cgcgtaggat aagggaaggt ggtccgctcc ggggtccaag
     1801 cgagcaaata ctcccgtaac gcggctggtg tgagtgttcg caagtcctgt tcgggtctgg
     1861 gagcacgctc accttcaaga acatctcttg cctctctcgt cgtcggtagc ggggcttcaa
     1921 tttcaccgaa ttcctccaca agagtggcat accgctcccg ccatgtcgcc gggagtgccg
     1981 tgtggcacgg ccacagtcgc ttccattgcc agctccggtg atgcttctcg atatcttcct
     2041 gggtagcgcg ccggccggtg ctcgtttcat agttcctgat cagttgctca atattctcag
     2101 gtccagcttc aacccaactg agaattgtct gttgttcatc aggagtcagg cgtgtgaaac
     2161 cgatatgaag gagggagtca tactcctttc caagatttct atctcggaac gaagcttcat
     2221 gtgttagcct cggggcgatc aagtctcttg gtgctgaagc tgtggtgaca agacagtata
     2281 aggccaatcg tcggaagatc agccaacgac gagcctcaag atcttcgacc aaaggaccga
     2341 gatctacgcg tccatcattg agtagcgttt cggccgcgtc tctaattctg actaccaaca
     2401 cgcttctgac atcatcgcga tcgtcttcat cctgaatact tagatctggc tgccagacac
     2461 gggagttatc gtcccattct tccgacgaat gtgaacggcg agacagtttc gctgctttct
     2521 gcaggatatt gcataaaacg tcaaacagct cctcccccct cctttctaat actgggcgaa
     2581 gatgctcatc cacgatctga atatattcat acatgctgat cctcggcttg accggcttgt
     2641 atgaatattc aaaatcatca tctgcccgca ccccttcgat agcgagcagc ggtgtcagaa
     2701 gcgtcagtgc ttcgtgcggc tttcctcctt ctgcaaggtg agttacgagt ttgcctagct
     2761 gtcgccagaa caaaaagcct ctgtcgtggt gagcccattg tttcagtctt ggcatgaaac
     2821 ttaccgaatg ttccgcggga accttccgca gtacatccac tacatcccga atcaccagcg
     2881 gattcaccaa agactgttga gtaagcgcat ccgcaatttg gctcaaagcc ttcatcccct
     2941 cggtaggatc ccctctgtct ataacacgca ctaggaactc tgaagccgcc caacgaggta
     3001 tcgcattggg ctccacagac ggaggtgcgt taaagatgcc ctcggctcgt agtgcgggaa
     3061 tccatgccgg atcatccaag tgctcaaaga agtatttgta atgctcaata tgtccgagtt
     3121 gagttactac aagatggacg tcagcagggg tcgacgtccg gatcagctca tccaggtcag
     3181 cgatcgttgc gaagaacccc tgaccaagtg tcagtactgt tctctcaaaa agaacaaatc
     3241 ttcgcttgat atcactccaa tcacattgag cgtctgtctt gccattatcg tgcgcgtgac
     3301 cgacaaacca atccttaagg tcggtccacc gcctcaaaac tggacgtagc gcatctccga
     3361 gatgacgatt ctctgggcga tttgcttgga aaagcctctc tgctatcgta tcgcgcttca
     3421 gccttgttgc cttatgctcc ttgatgagtt cttcaatttg attgaagaac tcttgagtca
     3481 gagcgtcctg gggatcagaa atcgatgtgc cccccatact cctcggagcc tgaatcggaa
     3541 gcctggtcaa tctgtgccgt gctagagcaa tcttgtccag gcgtttgtca tactcgagac
     3601 gggtgcgctc taccccggcg agaagagcgg gtaaggcatt ggcgatctct cggacgcaat
     3661 gcgcgatgat acggcttcgt cctggaaggg gttgctcctc gagaagagta acagcggttt
     3721 catagagttc ggccaaagat ggtgcttttt ccctcagcca atgacggagt cttcgtcgct
     3781 gtgtagtcca tgtaggggtt gatggcaaaa gcgggtgtgt ttcatctggc ggaggctggg
     3841 ggacagcggc aggtccatta ggcatagaca gggatgatta tatatatgcg acgaaatgat
     3901 ttctcaagaa atcgtagcgc cggaacggcc cccattaggc gcttggcggt cggctgaggt
     3961 ggccctcaac tccgcgtcgt ccaacgaggg ctttctgaag ccgcgcgtca agatctaatg
     4021 ctccctccac ttaccccctg cattcccctt gggagtagcc gcatcggcct tcactgcgcg
     4081 catgggccga gcacattctg atcgtgcgcg gtctgcgagc aagaaggacg gggcggctgc
     4141 tccctctctc aacacttcga tccatccgtc cggcatgcct ccgcacgatc gttgaaccct
     4201 gaattgcgca ggctttcgta cacattctgc tgccgttcca tttcccgatc gtagggagtc
     4261 ctttcccgca catccgactg atacccgccg tcggaatttc gccttggctg ccgcgccgcc
     4321 agtaccttgc gcacctcggt ttcataatag ccgcgcgttt gggggtcggg atctttctgg
     4381 tagcagatgt tcaagagctt gcggccttcg gcgtcatgtc ccacggcgta atgggcgaat
     4441 ccgacattgc agtaggcgtt cggcgtcgcc gggtcgagct tcaggacaat ttcagaatcc
     4501 tgcagggcgt tctgaaactg ttcgtggcgg ctgtagattt cggcgcgggc tttgtaggcg
     4561 ccgacatgtt tggggtcgaa acggatggcg gccgtgaaat cgtcgagggc ttcgcggtcc
     4621 ttcccttgat actcccggac gtaggtcaat ccgcggccat agtgagcttc ggcactggga
     4681 cgaagccgga tcgatgtcga atagtcgtcc aaggcttgcg gaatctgacc aagcatcttg
     4741 ccggccttgg cgcggatgag ataggcgtcg gccttgttcg gcagacggcg aatgacttcc
     4801 gaggcatcct gcagtgcctg cgcgtacgac ccgagaaaat aataacagcg ggcccgagcg
     4861 agataaatac cggtttgatt acgtttttgt tcgagtacgt gggtgagaag gtcgagggtg
     4921 tcttggttgc ccctgccgtt ggcttcgcag taggcatcca ccgaggcatc gccacaggcc
     4981 accgccagga gaaggccgca cagcgcgacc atactgccta cacaacgacc ggcttgtccc
     5041 atgcccgcct ccggagacgg gcgcattatc tgctcgtaga acaaacaacg tcaagactgt
     5101 catcacaact actacccaag agattatacg aggcaacgtg cggtggatgg ccacccctga
     5161 ttcgcgtccc agctcaactg acgaatcggc ctaaaatccc aaggagacac caaccgttcc
     5221 aagcaaggcc gcccgattgg caggaccgag aaattccgtc ccgagcccgc catccagcac
     5281 aacccgcgag gtgagctgat ggcgcaagcc gatttccaca ccggtgtggt ttcgctctcc
     5341 atgaagatcg gactgtctgg tgtagaggct ggcaatcaac gtgtcgcgaa aactccccgg
     5401 atatcccagc ggataactga ccgccgcgac ggcacggtag gtgccgggcc gctcctgtcc
     5461 ttgcggagag ccgagcacag tatacccgcc attgaggtgc acccgcagcc gcccgaacga
     5521 ccgggtcaga atcccggtca cttgcgtatc gacgcccttc gaattcacac cggtcggcag
     5581 atcagcttcc acgcgtaccg ccatggccgg cagaatcagc gtttccgtgt tgaagttgta
     5641 gagcaggccc agatggagat ccccggattt gttggctccc acaattgaat gagggtcagt
     5701 gaacaggtcg ccctgaattt cgatctgcgt gttggcgaac gcgccataga tgatttgcgg
     5761 ctgaaaggtg acctgcgtgc ggccttctcg acgatcgttg aatcgcacgc cgccttccag
     5821 gccgatttcc cctttgggaa tggcataggc atcctccatc ccgatggggc gattcgggtc
     5881 gaggttatca tggtccaacg ctagagcggg ctgggccaca cctgccaggc atgcgcagag
     5941 cagccaccgc acgattcgtg tgcccgcact cctcaatggt gatgctcccc ggccgtctgg
     6001 acgatcatgg gattcgtcgc atccgccgtc gccaggtaat aggtattcac gatgcgatag
     6061 atttccgccc gcttcgcctc ccacgtcggt aaccgttcgc ttgtaaggat atcgctgata
     6121 ttgtcatgca gcatgtgcag attgtcgaag atatgcgcga catccggata ccgtgcggcg
     6181 aactcggggc tcagttccgc ggtcaacggc atgaacgtcc actccacagg cggctgctcg
     6241 agataccggc gataggtcgt gagaatgggg cgcaccgctt gggtttttgc cgtcaggtcg
     6301 cgagccgcca gcaacggatc gtataccgcg acctgcagat agtggtacga ccagatggtc
     6361 gcgttgaaga gagggaaccg ctgccggaac gctttggaat agggaaacga atccaggcgg
     6421 cggtggtcca attgcttgga cgtgatggcg tacgcgctct ctttataata ggccaataca
     6481 ttccggatcg cacgatcctt gtccggctca tccgacaccg caatgtcata ggtggcgcgg
     6541 tgaagggcat gcgcttcgtc gaacgtgttc tgcgcgcgcc aggccagctt catataggtg
     6601 ggggcgatgg cttcttcgtt gggattcaac cgcggtctgg tcgcgataaa cgcaagggtt
     6661 tccttgcgcg cccgatcctc gatagccgcg acatcctgac cgccggtcag cagcaggttt
     6721 tcatacaaat tggaatggcc gaagtcgacc ccattaaact cgctgtccag ttcggccaga
     6781 tcttcccgga gcgaaaaatt ccagagggca cggtaataga accgcttgtc gcgcggctca
     6841 aactgattgc acccggccag cgcgaaggcg accgtggcta tcgccacgat cgccatcctc
     6901 ccaaggatag taccgccaga tttcatgtcg gtcgttccaa ctccatacgt cttagtggca
     6961 gtggtgccca tgcaccagcg catggccttt gccccgcgcg atcaaccatc gattgacggg
     7021 aaaggccgcg attccagcca cgatcagcga gaagcccaga ctgccccaga acagcaggtc
     7081 aaatatcccg gcgtccatcg cgccgggaat gaccatcatg atggcattgt cggcaagttc
     7141 catggtcgtc atcgacaacg tatctgatgc cagggccaac cccagcgccg ttccccatgc
     7201 cataccagcc cgccgcaagg gatagacggt catggcatag ccgaagaaga acgccaacgc
     7261 aatagcgagg gcgatcgtag accaattgcc ccagcccagc gccgtgccga ttgccattcc
     7321 gagcagttcg ccgatcgtgc acccgctcaa gcagtgcaag gtcgccatga gcgccgtgcg
     7381 gtcgagcgag ggtgtcggat gcctctctgc actgtgcgcc tgatgcggtg catggccatg
     7441 aggctgtggg cgttcatggt catgatgatg aacctgactg tctgactctg cctgccgtgg
     7501 tacggctgcc gctgcggaat gatgatgcct catgcgcatc ctccctgtaa gtggtgactg
     7561 ataccgtgca ccactacaga cggaaaaagg cggaaatcat tacagggacg aaaggacacc
     7621 agttcgcagg gggtgcggac tgaatcaccc atctcgtcgc tctcactgat tgttcgaata
     7681 cgtgtatttc tgactcattg aatcccctcc tgctgtttgc ttttgcgaca acgcgggaaa
     7741 aagtcttgat ctcaaccttg gagacatgct tgactgaacg ctgttccgca ttcttctcag
     7801 tttactggag ggtttgcatg ccgagtggaa tccatcgcac tctctatacg ctcttctttc
     7861 ttctcctcgc tcccctcacc actcaagccg ccgacctacc taataccagc ctcacgcctc
     7921 tcagatttct ggtcggagaa tggaaaggga cggatgcgga cgggaaagct cacaagagcg
     7981 ccttcaccct gagctccggc ggcacgaccc tcaccgaagt cctcacgcct cctgatagcc
     8041 ccccgatgac gaccatgtac tacagcgacg gcgaccaact gatgctgacg cattattgct
     8101 cgctcgataa ccaaccgcgc atgcgcgccg ccgcggtcaa agacggagat aagaccatca
     8161 cgttttcttt cgtggatgcg acgaatctca agaatccgac cgatgtgcac atgcatcagc
     8221 tggccatcga cgtcaaagac cacgaccatt tcacccaaac ctggaccttg agcaaggctg
     8281 gaaaggacgt gccgaaggtg tttacctttg agcgcgtcaa atagctttca cgtcgtggat
     8341 caacaaggag cctcccgatg ccatcacaaa cgccagcagc cgtgatcgaa gcccagcgtg
     8401 aggattggaa ccgcgtcgcc gccggctggg ataaatggga tcagttcttc aatcgcacga
     8461 tggccttcat caatcaccgg ctggtggcgg atgcgcgtat tcgtccgggg cttcgggtgt
     8521 tggacttggg gtccgggacc gggtatccgg cgatcttggc cggtgaagtg gtgggagctg
     8581 acggaacggt ggttggaatg gatctcgccg aatcgatgct ggccgtggcg acacgcaagg
     8641 caaagacgct cggcctgcac caggtcagct tccgaaccgg agacgtgacg tcattgccgt
     8701 tcgactccgg ctcgatcgat tcgatcatca gtcgattctg tttgatgttc ctcccggaaa
     8761 ttcccaaagc cacagcggaa atcgcccgcg tgctgaaacc gggaggatat gtcgcggccg
     8821 ccgtctggtc gagccccgac aacaatccgt tcattcgtat tccaatggac gtgatcaaaa
     8881 ccatcctgcc gctgccgccg ccggaccctg aggcgccggg gatattccgc cttgcgcgtc
     8941 cgggcgacct ggccggcatg ttcgagcgcg ccggtctcac ggtgctggac gacgaggagt
     9001 ttacggccga agtgacctat acgactgcag aagaattttt tcgcgggctg atggatattg
     9061 ccgcaccgat ccaaaacctc tttgccaaac tgaccccggc ccaacaaacc gaggcggagc
     9121 gcgggattat caaaacggtg aatgagtata aacgagcaga aggggtctcg ttgccgattg
     9181 ccgtgcggat tgtgagtgcg cgcaagccga agtagaacga taagtgaggg cgcctgcccg
     9241 cgacttgtgt gtgtcacggg cgggcgccgc actcttctaa atctagtccg tggctttttc
     9301 ttttaattgc ttcatctttc ccttcgcccg ctccttggcc cccttcatct tgcccttggc
     9361 ccgctccact tcggccttcg tatcattccc tttcagttct tcattcatgg ccttggtttc
     9421 gcccttcatc tcttccacat tacccttcat gtcacctttg gccttctcca tcttggcttt
     9481 ggtctcactc gcatccgccg agaaaaccag tgccgtcgag aaacacacta ctaccgccgc
     9541 gactgtcatg aatgtacgca tatccgtcct ccgagtatag gtgaaccgct gtcactaatc
     9601 tgcgggatcc gtctgcatcc ccccactaga gaatccccta gtatcgagat gaatccgtcc
     9661 cccctactgc ccagggttcc ccccagttga accactgcgc aggccgcgac acactcgtct
     9721 cgacagcctg tgccttcatg tctcagaaag gagccagctc atgcgacacc tcattctttc
     9781 cggcctcctg ggcttcgtga tcctcacgac cagcggatgc agttatctgt tctatcctca
     9841 cgccaaggag tttacggaga aggcgaaggg tggaaacacg gttgagacgc tgatcaacct
     9901 gacgaatatg atggaggcat ctgcgaaggc cggacagcaa ggcaaaggca aagaccaggc
     9961 gcttgatgat ctgcacaatc agctgcacgc gtttaacaac acgatttgct gtgtcgacaa
    10021 gaataagcag gagacgccgg cctatgccct ggcggtcacg cacaataagg aactctgggc
    10081 gattttcaaa cgggtctgga agttcaagga tgatcaaccc caacgcagcc agcatttgga
    10141 gctgtttgtc acagaagtgc gggaactccg ggagtcactc caagcattga aatgaaggcc
    10201 ggaatgttgc acagtgcgcc acggcgggga gccagccttc ctgactctcc gccggtgcaa
    10261 cgttcacgat gctcgcgcgg ttcgtgagac tgcccctgtc gaatcgactg gtgaaggcag
    10321 tccatgtcca cgcggcgagc gtcgaaaggg tctctggcgc atgcgtcccg acagtttcaa
    10381 agacttcgtg gtggaccaac tcggtgaggt cccgcaattg atgtgcaaag ccatgttcgg
    10441 cgggcacggt ctctatcagc gcgagacgtt ctttggcatc attcacaaag gccggctcta
    10501 catgaagaca aacgggatga cacaatcggc ctataccgcg cgaggcatgc agccgtttcg
    10561 cccgaacggc aagcaactcc tcagacatta ttacgaagta ccggtggata ttctggaaga
    10621 tgccgagcag ttgacggatt gggcgcttcg ggccattacc ccaccgacgg cgcaattgat
    10681 tcttcccttt cacgacaatc cctcgatcaa ggacgttctc acgcacatgt gatgaaccag
    10741 gaactcacca cgccttcccg acgcctctgg actatcgggc actcgacccg gtcaagcgac
    10801 gagtttctcg ccctccttca cgtccacgac atccaacgac tcattgatgt ccgtcgtcat
    10861 gccggctctc ggcgatatcc gcactttcat tcagatgcgt tagcccagag tctccccatg
    10921 gcgggactat cgtacgaaga actgccggcc ctcggcggcc gtcgaacggc ccgagccgac
    10981 tcgcctaaca gcgggtggaa gaatgccagt ttccgaggat atgccgatta catgcagacg
    11041 gaagagttcg acagggcact cgaggagctg atggcccaag gcgagaatga ccggacagcc
    11101 atcatgtgcg cagaagccgt gccctggcgt tgccatcgtt cattggtggc cgatgccctt
    11161 gtcgcacgtg gttgggacgt cacccatatt ctgggcggag gccaggggaa gccgcaccag
    11221 ctcacaacct tcgcaatgat ccaggccggc cgtgtcatct atcccgcccc ctctgatcag
    11281 tgctccactc tccggatgtt ttagacattt cgctgctcat tgtgttcatt ccgacgttac
    11341 tgcacactct acgcgatgtg gtatcgaacc ggcgccgact tcgtcctgct cctgcatgta
    11401 agctttgtct tattcgtgat tgctggcggg ctcctgttgt tgaaatggcg aggactggcc
    11461 tggatccacc tgccggcggt ggcctggggc gtcctggtgg aactgaccgg ttggatctgt
    11521 cctttgacgc cgcttgagaa tcacttgcga agtctggcgg gagaatcgtc ggcggagggt
    11581 gacttcgtcg gccgctatct tccatccctg ttgtacccgg cggcactgac gcgcgagatt
    11641 ca
//
LOCUS       DAOQEW010000117        11529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1675_length_11529_cov_7.771309, whole genome shotgun sequence.
ACCESSION   DAOQEW010000117 DAOQEW010000000
VERSION     DAOQEW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_11529_cov_7.771309"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLS35_12735"
     CDS             complement(<1..511)
                     /locus_tag="PLS35_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19580.1"
                     /translation="MSSHQSSDRPEPRKSGIHDADLDGLAISRPAPGGIPSSGGRRWR
                     WLTLAVLLAVAGLAWSTAWLRPDQRVEVVPVVRMPSGSGAGLAATGYVVAQRQASIAS
                     KGTGRLIYFGVNVGDRVKEGQLIARIEHDDMDALYRQALARLGVARAQLGAAKPELEE
                     ATLSFERVKT"
     gene            complement(592..1812)
                     /locus_tag="PLS35_12740"
     CDS             complement(592..1812)
                     /locus_tag="PLS35_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNI19581.1"
                     /translation="MPSESIPVAARQAAQAGVRDRRARVWRTWWVQAHLYLGLTTGAL
                     LVVFGLTGSILVFFQEIDEWLNPALLTVDATADGQNSYRPIGEILAAAEQAAAPGSRI
                     TQVYGATKPERVMAVYMEEPSKAWQRIFVDPYRARVTGVRSYGPDEWVPKYLMDAIFA
                     LHYQLFVGMTGVTLAAIAAWLLILSLVTGVIVWWPVNGQWRHAFVIRRPFALFRLLFD
                     LHKTVSLYLCLAIGAVLLSGAYMNWAEPIIWITQVLSPATRGPAQSAPSAVEDGAHPI
                     SPQRAVELAAAIYPEGRLTSIAMPEDATGVYQVGRQGVPGLSAFWSERIVSVDQYSGS
                     ILDVRAPDTRRSAGETFLDWQWPLHSGQAFGIPGRLLVCASGLACPVIYATGFLMWWR
                     KRRGRARRSLVPAS"
     gene            complement(2022..2456)
                     /locus_tag="PLS35_12745"
     CDS             complement(2022..2456)
                     /locus_tag="PLS35_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19582.1"
                     /translation="MVVRLKLYRVFVVNELLHIMSLRGRTGRAPSLLFFVACLLVAIR
                     VAAGFVCHTCFIDFEKPTSRSFHLHAGGDRDPCHHGQAEASPLTTWACTVTQDETAFI
                     LPEIPRLPLVVSEFVPLVLLLLSYRTLFQIAAHGRGPPVPLS"
     gene            complement(2614..4962)
                     /locus_tag="PLS35_12750"
     CDS             complement(2614..4962)
                     /locus_tag="PLS35_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNI19583.1"
                     /translation="MKSTDSCQNASRSFIPLRRGSAGIGRIVAVAGLTLLTACIGPHK
                     PVATDKTSATPVAAPAQGESTGESGGDKAAQARQSPPALPVPLPVPVPAEDLAGTGAS
                     TRDDAPVVDVKPVHIVAQAESYHVTNTVTATKTDTPIMDTPVSVKVVPQQVMRDQQVV
                     RVDQALKNISGVISGAEGDMQFNMRGFDQFNFYRDGYPFQSQWFHGEDLTNIERVEVL
                     KGPGSILYGRAEPGGIINFVTKQPLDQPYYSLQQQVGSYGWYRTNADLTGPLTANNSV
                     SYRLNIGYQTNQTFQQFGGNERLIVSPQVRWRITDHTTSTLKYEYSDISLTGKGNIPL
                     LGNRPAPIARELNLGERWNLQNDKYHLVSLITEHAFNDAWSIRHRFNFSNHQLTLTGQ
                     NVGNAAANGDVSRFFFAQNTDGRDYTRNVFNALELTGHVTTGRIKHTLLFGGDYLHND
                     IRATMAGFTPATDDVINIFNPVHTAGPPVIAPGDVTKYTWSLPWFGLYAQDQIELPYH
                     IHILAGFRYDNAKTSTSNENLGVRTPTIKDTNERISPRAGVVWRPVPEFSLYGSYKEN
                     FGASNTFTAGSVVPLPPQTAQQWEVGAKTELFGGRFIGSLAYFDLTKQNLPVFFPGET
                     RAVGEGESRGVEFDVSGEILPGWSVIGAYAYTPFAKTTKDDPALGTMGKRLNNAPVNS
                     GSLWTVYQFQEETLQGLRVGVGVQGVGERQIGFGETAQAPGYVITNLMASYHWRVGTA
                     RYTAQLNVDNLLDKSYIGQVFSYGPTYYGMPRFFMGSIRMEF"
     gene            complement(5189..5593)
                     /locus_tag="PLS35_12755"
     CDS             complement(5189..5593)
                     /locus_tag="PLS35_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19584.1"
                     /translation="MKPARPFMPLSGSRGRGQRHAVLLALFLIGIRLVAGFLHVSCFN
                     EMERPSARSFHLHAGGDRVPCHHGKAPTSPLSDWACSVNQDESAYILPEVPLLPLVAA
                     LFLLFLLFRVSFGSRSAIVPHGRGPPRHLLVS"
     gene            complement(5756..>6829)
                     /locus_tag="PLS35_12760"
     CDS             complement(5756..>6829)
                     /locus_tag="PLS35_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI19585.1"
                     /translation="KPEIVWGMDFSSQHGAQSSTLYDQSVFDGSGGRVFSKSGEVASS
                     PSRVFNSTAVFAQGEWTPLAALVLRGGVRYERVKVTSSDFTGNGSFLAASAIEFDTTV
                     FNAGFVYHLLDPLDLFFNFSQGFNVPVGSIDNALGNVPPGTAISLQRLQPQRVNNYEL
                     GLRGKWSKVQASLSGYISKSSLGLNFDQFTNSLRRAPQNIYGVEATLDLQPIERVRLG
                     GTYTFVEGDQTIAFNPDVRVPLNGFSIAPQKITAYLEHLTVPEWEWRNRIQVLYSGSR
                     SRSLTAFNSGIDPTADPFGQTEYFLVDLISTVKAGPGTLRFGVENLLNKQYATPFNQI
                     SFTNSFYFAGRGTTASIGYSIYY"
     assembly_gap    6830..6929
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6930..7795)
                     /locus_tag="PLS35_12765"
     CDS             complement(<6930..7795)
                     /locus_tag="PLS35_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNI19586.1"
                     /translation="MNMCLFDPSKRVLVTVSLLMLCSGGPLWAAEPEVVESAEEIPVV
                     NASDVLISATRTPTSMGSVNQAVTIVSEEQLRQQLAASPSRSITQILTKLVPGMSQND
                     QSMDSGTSLKIRGRSIAILIDGVPQQSSPTTELDLAHIDPSAIERIEIIRGSTAIYGN
                     GAMGGLINIITKQPGEGKPSYYTDVQGSGSLTRVLRGLSGTVVQGFQGKKDWFDYNIV
                     GSFNHQGGMFDAEGSRTMPGTNTVGGFADTKNYNIMSKFGATFGDHRFQLTFNRKQSW
                     QDTNYLVDPNVDA"
     gene            complement(8011..8409)
                     /locus_tag="PLS35_12770"
     CDS             complement(8011..8409)
                     /locus_tag="PLS35_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19587.1"
                     /translation="MIDLLRIMHSSRRSDWISRLLAVMACLLIGVRLVGGFVCNTCYL
                     DFEKPASRSFHLHGGGDREPCHHGQPEASPLIQWACTVMQDESAFVLPDIPRLPVVIS
                     LFVPLVLLLVSYRTLFPIAAHGRGPPAGRF"
     gene            complement(8518..8892)
                     /locus_tag="PLS35_12775"
     CDS             complement(8518..8892)
                     /locus_tag="PLS35_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNI19588.1"
                     /translation="MEREVDQINVIPLVDIMLVLLVIVLTTATFITTGQIPVNLAKAS
                     ASSDRQDRPIVVTVTAEGTVFVEDHAVTEEQLDTALAPHPRQSSVLVRADKVTRLERF
                     VAVVDRIRGLGFQQVSLEVFRA"
     gene            complement(8882..9307)
                     /gene="exbB"
                     /locus_tag="PLS35_12780"
     CDS             complement(8882..9307)
                     /gene="exbB"
                     /locus_tag="PLS35_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HNI19589.1"
                     /translation="MESLKFGVEYGVIGLLGLLGVWALIIAIERWHFYRRVDPSAFVT
                     LDTFEMALTKRLVVIGTIAANAPYIGLLGTVLGIMLTFHTMGTSGAMAVSSIMVGLSL
                     ALKATAVGLLVAIPCVVMNNVLRRRVTELMTRYEARHGA"
     gene            complement(9533..11506)
                     /locus_tag="PLS35_12785"
     CDS             complement(9533..11506)
                     /locus_tag="PLS35_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNI19590.1"
                     /translation="MPAIYGLVTVLLLVWSMVPVYAEPGSAPLPTAAFASLPRIESIQ
                     LSPSGRHLAVLRHHDGQTFLETHTVTGQDAHPVVSTDNREYIITWFRWINDERLLVSM
                     RFAATPEATDTVETRLMAVNRDGTEQTANLLKQGGFSSLFGQKHVPQFEYQLVGMIPG
                     DPRHVLLALDLEHPNAPDVYKVDVYSGERQLVQANPSSKPDRNAISHWIADRTGAVRS
                     GVGQFQTAVHAIVRQPESTLWRELADYDLAKETGLVPLAFDADPAWLYVRDQHRGKTA
                     IFKLNVMDRAADRVLVVADPKFDLNGELVYAPGRKKVVGVRYSAADERVLFWDFDAQR
                     LQARIDRAIPGTVNVIHSSSADGRLHVVKSSGAAQPPRWSIFDEQDGRMVLLGRSYPD
                     LETAALAAPTTTYVTARDGKELQVFLTVPKDRDPRHLPMIVFPHGGPAARMPGAFNYW
                     TQWFVSRGWAVLEPRFRGTEGYGDDVLRAGFQRWGLEMQNDLTDVVQYAVRAGIANAG
                     RICIVGSGYGGYAALMGVVKTPDLYRCAVSLGGVTDLPQLVSDGRGYLNQKPMVEARI
                     GSWWNDRDRLRETSPVAHAQGMRTPLLLMHGSMDRSVPVSQSRNLADALKAANATNAR
                     YVELPLADEALRREEDRLHVFSEMERFLTQHLD"
BASE COUNT         2449 a         3598 c         3274 g         2108 t
ORIGIN      
        1 gggtcttcac ccgctcgaaa ctcagcgtcg cctcttcgag ctccggtttg gcggcgccca
       61 attgtgcccg cgccacgccc aaccgggcca aggcctgccg gtacagcgca tccatatcat
      121 catgctcgat gcgcgcgatc agttgccctt ctttcacccg atcccccacg ttgacgccga
      181 agtagatcag ccgccccgtg cccttggagg caatcgacgc ctgccgctgc gcgaccacat
      241 acccggtcgc cgccaggcct gccccgctcc cggatggcat gcgcaccacc ggaaccactt
      301 caactcgttg gtccggtcga agccaggccg tactccaggc caagcccgcc acagccagca
      361 ggaccgccag cgtcagccac cgccaccggc gaccaccgga agacggaatc ccaccaggcg
      421 cgggacgaga aatcgccaac ccgtctaaat cggcatcatg aatgccggat ttgcgaggct
      481 caggacgatc agatgattga tgactggaca cgggaacctc ctccatggaa cggcagtgag
      541 tcgtgccgct ccttctcgtt tccttcgcac tctaatctgc cacaccgcgg gctacgaggc
      601 cggtacgagc gaccgacgcg ctcgcccccg tcgcttgcgc caccacatga gaaacccggt
      661 ggcgtagatg accgggcagg ccaggccgct cgcacagacc agcaagcggc cgggtatccc
      721 gaacgcttgc cccgaatgca ggggccattg ccaatcaaga aaggtttccc cggcgctccg
      781 cctcgtatcg ggggcccgca cgtcgagaat tgagccactg tattgatcga cgctcacgat
      841 ccgttccgac caaaacgcac tgagccccgg caccccctgc ctgccgactt gatacactcc
      901 cgtcgcatcc tccggcatgg cgatggaggt caaccggccc tcgggataga tggcggccgc
      961 caactccacc gcccgctgcg gactgatcgg atgcgctcca tcctcaacag ccgacggagc
     1021 agattgcgca gggccgcggg tggccggcga cagaacctgc gtgatccaga tgatcggctc
     1081 ggcccagttc atataggctc ccgacaggag cacggcgccg atcgccagac agagatacag
     1141 cgacaccgtt ttgtgcagat cgaacagcaa ccggaacagg gcgaacggcc gtcggatgac
     1201 aaacgcgtgt cgccattggc cgttcaccgg ccaccagacg atcacacctg tgaccaagga
     1261 caggatcagc aaccaggccg cgattgccgc cagggtcacg cccgtcatac ccacgaacag
     1321 ttggtaatgc agtgcgaaga tcgcatccat caaatacttg ggcacccact cgtcaggccc
     1381 ataactgcgt acccccgtca cccgggcccg gtagggatcg acaaaaatcc gttgccaggc
     1441 tttcgacggc tcctccatat agacagccat gacccgctcg ggtttcgtcg ccccatagac
     1501 ctgcgtgata cggctccccg gcgccgccgc ctgctccgcc gccgctagaa tctcacctat
     1561 cggccggtag ctattctgcc cgtctgcagt cgcgtccacc gtcagcaacg caggattcag
     1621 ccactcgtcg atttcctgga agaacaccag gatactgccc gtcaatccga agaccaccag
     1681 cagcgcccct gtggtcagtc ccaaatagag atgcgcctgc acccaccacg ttcgccacac
     1741 tcgcgcgcga cggtcccgta ctccggcttg ggcggcttga cgagcagcaa ctggaatcga
     1801 ctcagacggc atggctctcc cagacatgcg attcacattc ctccgccgac ggtctatcga
     1861 ggcagcgtgg cggtagtggc gacacctccg gcatgggtgc ccttgtttca ccgacgaggc
     1921 gacgacgcgc aggacggacg aacactcatt catgacgcgg gtgacgatca gctcgtcacc
     1981 acgcgggaat tgtccttatt cagtgcggat aaaaccgatg atcaggaaag aggaaccgga
     2041 ggaccacggc catgggccgc gatttgaaac agggtgcgat acgacagcag caacagaacg
     2101 agtggaacaa attcagaaac cacgagcggg agtcgtggaa tctccgggag gatgaacgcc
     2161 gtttcgtcct gggtcaccgt gcaggcccag gtcgtcagcg gactggcttc cgcctggccg
     2221 tggtggcagg gatcgcgatc ccccccggca tggagatgaa aactccggga ggtcggcttt
     2281 tcgaaatcga tgaagcaggt atggcagaca aacccggccg ccacacgaat ggccaccagc
     2341 agacaggcga cgaaaaagag cagcgacggg gcccgccccg ttcgcccacg gagagacatg
     2401 atgtggagga gctcgttcac cacaaacact ctatacagtt tcagacgcac taccatacga
     2461 aactgccacc ctcatgtcca ctacctgttg ctcacaggtg ctacagtcca caccggggtt
     2521 tcgtggtgca gaccgcatcc ccccggcggc tcgcgacatg tcgtcgtgaa ccactcttgt
     2581 ttccgctccc gtcaaacttc acgtttcacg cccctagaac tccatgcgga tcgaccccat
     2641 gaagaaacgc ggcataccgt agtaggtcgg accatacgag aagacctgac cgatatagct
     2701 tttgtcgagc aggttatcga cgttcagctg tgccgtgtac cgggccgtgc cgacacgcca
     2761 gtgataactc gccatcaggt tggtgatcac atatcccggc gcctgggccg tttcgccgaa
     2821 cccaatctgg cgctcaccca ccccctgtac gccgactccc actcgcaatc cttggagcgt
     2881 ttcctcctgg aactgataga cggtccatag actgccgcta ttgacgggcg cgttgttgag
     2941 gcgcttgccc atcgtcccaa gcgcgggatc atcctttgtg gttttggcaa agggcgtgta
     3001 ggcataggcc ccgatgacgc tccagcccgg cagaatttcc ccgctgacgt caaactctac
     3061 accacggctc tctccctcgc cgaccgccct ggtttccccg ggaaaaaaga ccggcaaatt
     3121 ctgcttggtc aggtcgaagt aggccaatga gccgataaat cgaccgccga acagttccgt
     3181 cttggccccc acctcccatt gttgagccgt ttgcggcggc agcggcacaa ccgaaccggc
     3241 ggtaaacgta ttcgaagccc caaagttttc cttataactg ccgtagagcg agaactccgg
     3301 taccggacgc catacgacgc cggctcgtgg cgaaatccgc tcattcgtat ccttgatggt
     3361 cggcgtgcga acgcccagat tctcgttgga ggtggacgtc ttcgcgttgt cgtaccggaa
     3421 tcccgcgaga atatggatat ggtagggcag ctcgatttga tcctgcgcgt agagaccgaa
     3481 ccagggcagc gaccacgtgt atttcgtcac gtcaccaggc gcgatgaccg gcggtcccgc
     3541 cgtgtgaacc gggttgaaga tattgatcac gtcgtcggta gccggcgtaa accctgccat
     3601 cgtcgcgcga atgtcgttgt ggagatagtc tcccccgaac agaagcgtgt gcttgatccg
     3661 gccggtggtg acgtggccgg tgagctcgag ggcattgaac acattgcgcg tgtagtcccg
     3721 accgtctgta ttctgcgcaa agaagaatcg actcacatcc ccgttcgccg cggcattccc
     3781 cacattctgt ccggttaacg tcaattgatg gttgctgaaa ttgaaccgat gcctgatcga
     3841 ccaggcgtcg ttgaaggcat gctccgtgat caatgagacc agatgatatt tatcgttctg
     3901 caggttccag cgttcgccga gattcaactc ccgcgcaatg ggggccggac ggttgcccag
     3961 cagaggaata ttacctttgc ccgtgagcga gatatcgctg tactcatact tcagagtaga
     4021 ggtggtgtga tccgtgattc gccatcgcac ctgcggggac acaatcagcc gttcattgcc
     4081 cccgaactgc tgaaacgtct ggttcgtttg atagccgata ttcaatcggt aggatacgga
     4141 gttgtttgcg gtcaacggac cggtcagatc ggcgttggtc cggtaccagc catacgagcc
     4201 gacctgctgc tgcagcgaat aatagggctg atccagcggt tgcttggtga cgaaattaat
     4261 gatcccgccc ggctcagccc gcccgtagag aatggagccc gggcctttca agacctccac
     4321 ccgctcgata ttcgtcagat cttccccgtg aaaccactga ctttggaagg gatacccgtc
     4381 acggtagaag ttaaactggt cgaagccgcg catattgaac tgcatgtcgc cctcagcgcc
     4441 gctgatgaca ccgctgatgt tcttcaaggc ttgatcgacc cgcaccacct gctgatctcg
     4501 catcacctgc tgaggcacaa ccttcaccga caccggtgta tccatgatcg gggtgtcggt
     4561 cttggtcgcc gtcacggtgt tcgtcacgtg ataggactcg gcttgagcca caatatgcac
     4621 cggcttgaca tcaacaacgg gagcatcgtc ccttgtggat gcgcctgttc cggccaagtc
     4681 ttcagccgga accgggacgg gaagcggcac gggcagagcc ggcggagact gccttgcctg
     4741 cgccgccttg tctcctccgg attccccggt cgattctccc tgagccggtg cggcaactgg
     4801 cgtggcagaa gtcttgtccg tcgccaccgg tttgtgcgga ccgatacagg ccgtcagcaa
     4861 cgtaaggccg gctacggcga cgattctgcc gatgcccgcg gaaccgcggc gaagaggtat
     4921 gaaagatcga gatgcgttct gacacgagtc cgttgacttc atcgacaaca ctcctccttc
     4981 ggttcccgct ctacacgcac ggaaccggaa ctcgccgggg gcagaccggt gacgaaggct
     5041 gagcggctgc acgcagggtg tacggggctg gtccatcaaa gacctgcccc ttccggcatc
     5101 ggatgatgcc ggttccctgg cgtgcggccc gccttcggtg cagcggccgt gcgactccca
     5161 actaggtgat atggatgaga accctgcgtt acgagacgag gaggtgacgg ggtggcccgc
     5221 ggccgtgagg aacaatcgca gaacgactcc cgaacgagac tctgaaaagc aggaacagaa
     5281 gaaacagcgc cgccaccagt ggcagaagcg gcacctccgg caggatatag gccgactcgt
     5341 cctgattgac ggaacaggcc cagtcactca gcggactggt cggcgccttg ccgtgatggc
     5401 agggcaccct gtctcctccg gcatgaaggt gaaacgaccg cgctgacggc cgttccatct
     5461 cgttgaagca actgacgtga agaaatccgg cgacgagacg aatgccgatc aggaacaagg
     5521 ccaacagcac cgcatggcgc tggccgcggc ctcgagatcc cgacagaggc atgaatggac
     5581 gggcaggttt cacagaacat tccggtagaa cgtaaggggc ctcaccctac ggaaccgcac
     5641 gcagaattgt ctaccgatgc ctgctcacgc ttgctacaca gctctctgaa acactcggac
     5701 aggcccggca ttgtgaccaa tgccgggcct atatcaagaa cgattcggaa ctggatcagt
     5761 aataaatcga atacccaatc gaggccgtcg ttcctcgccc cgcgaaatag aagctattgg
     5821 taaacgaaat ctggttgaac ggggtcgcgt actgtttgtt caacagattc tccaccccga
     5881 accggagcgt gcccggcccg gctttgacgg tgctgatcag atcaaccagg aaatactccg
     5941 tctggccgaa gggatccgcg gtgggatcga tgccggaatt gaatgccgtc aacgaccgag
     6001 agcggctgcc ggaatagagc acttgaatcc ggttgcgcca ttcccactcc ggcaccgtca
     6061 ggtgttcgag gtaggcggtg atcttctgcg gcgcaatgga gaagccgttc aacggcaccc
     6121 ggacatccgg gttgaaggca atggtctgat cgccttccac gaaggtgtag gtgccgccca
     6181 ggcgcacccg ttcgatcggc tgaagatcaa gtgtggcttc caccccgtag atgttttgcg
     6241 gggcgcgccg caacgaattc gtgaactgat cgaagttcag ccccagcgac gacttggaaa
     6301 tgtatccgga cagcgaggcc tggaccttac tccattttcc ccgcaacccg agttcataat
     6361 tattcacgcg ctgcggctgt aaccgctgca gcgaaatggc ggtgcccggc ggcacgttgc
     6421 ccaacgcatt atcgatcgat ccgaccggca cgttgaaccc ctggctgaaa ttgaagaaca
     6481 ggtccaacgg gtccagcagg tggtagacaa atccggcatt gaagaccgtc gtatcaaact
     6541 cgatcgcact cgccgcgagg aaggacccgt tgccggtaaa gtcgctgctc gtcactttga
     6601 cacgctcata ccgaaccccg ccgcgcaaga ccaacgcggc cagcggagtc cactcgccct
     6661 gcgcgaacac cgcggtgctg ttgaagaccc gcgagggcga cgaggccacc tcgccggact
     6721 tgctgaacac gcgtccgccg gatccatcga agaccgactg atcgtagagc gtcgacgact
     6781 gggcgccatg ttggctggag aagtccatgc cccacacgat ttccggcttn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgtccacatt gggatccacc aggtagttcg
     6961 tatcctgcca ggactgcttg cggttgaagg tcagttggaa ccggtggtcg ccgaaggtcg
     7021 ccccgaactt gctcatgatg ttgtagttct tggtgtcggc aaatccgccc accgtattgg
     7081 tgccgggcat ggtacgggag ccctctgcat cgaacattcc gccctggtgg ttgaagctgc
     7141 cgacgatgtt gtagtcgaac cagtcttttt tgccctgaaa gccctgcacc acggtcccac
     7201 tgagccctcg taacacgcgg gtaagcgatc cactgccctg cacatccgtg tagtagcttg
     7261 gtttaccctc gcccggctgc ttcgtaatga tattgatgag accacccatc gcgccgttgc
     7321 catagatcgc ggtggagccg cgaatgattt cgatgcgttc gatggcgctg ggatcgatat
     7381 gagccaggtc caattccgtg gtcgggctgc tctgttgcgg gacaccgtcg atcaaaatcg
     7441 caatcgaccg gcctctgatc ttcagcgacg tgccggaatc catcgactgg tcgttctgcg
     7501 acatgcccgg taccagcttc gtcaaaatct gtgtgatact ccggcttggc gaggcggcca
     7561 gctgctgccg caattgttct tccgacacaa tggttacggc ctgattcacg ctacccatcg
     7621 aggtcggcgt gcgagtggcc gagatcagta cgtccgaggc attcaccacc ggaatctctt
     7681 ctgccgattc tacgacttcc ggttcggccg cccacagagg ccccccagag cacagcatca
     7741 acaggctgac tgtaacaagg actcgttttg agggatcaaa caaacacatg ttcatggtgc
     7801 acgacttcct ttccttcacc gacgcatcgg cctctaccca aggccgacgc agcacggacg
     7861 acaccgacga gccctgcagc gatagcgacc gtcggatctg cttgagaacg agaacccact
     7921 cattcacgac agagccaggg caccactggg ccacatcgga caacacaggc gtccgacttg
     7981 cggttgttca ttgatgttga agcgaaatga tcagaaccga ccggcaggag ggccgcgtcc
     8041 atgggccgcg atcggaaaca gcgtacgata ggacacgagc agcaggacta gcggcacgaa
     8101 cagcgagatc acaaccggca ggcgaggaat gtcgggcaag acaaaggcag actcatcctg
     8161 catcacggtg caagcccact gaatcagtgg actggcttcc ggctgcccgt gatgacaggg
     8221 ttcccggtcg ccaccgccat gaaggtgaaa gctgcggctc gcaggtttct cgaagtccag
     8281 atagcaggta ttgcagacga agccgcccac gagacgaaca ccgatcaaca ggcaggccat
     8341 cacggcgagc agacgtgaaa tccagtccga gcgtcgagag gaatgcatga tgcggagcaa
     8401 atcgatcatg aaccaacctt cacacaggaa ggcggccact ctacgaaagg gcaggaaaaa
     8461 ctgtctacag acgggtgctc acgggtgcta cagcgtgcca cacaatcggt gtcgccctca
     8521 tgcccggaac acttccaaac ttacctgttg gaatccgagc ccacggatcc ggtcgacgac
     8581 ggcgacaaac cgttctagtc gcgtcacctt gtcggctcgg accagcactg acgattgacg
     8641 aggatgcggc gccaaggcgg tgtccagttg ttcctctgtg acggcgtgat cttccacgaa
     8701 gaccgttccc tccgccgtaa ccgtcacgac gatcggccgg tcctgcctgt cgctcgacgc
     8761 cgaggctttg gcaagattga ccgggatctg ccccgtcgtg atgaaggtgg ccgtggttag
     8821 cacgatcacg agcaggacga gcatgatatc gaccagcgga atgacattga tctgatcgac
     8881 ctcacgctcc atgccgcgcc tcatagcggg tcatgagttc ggtcacccgg cggcgtaaca
     8941 cgttgttcat caccacgcag ggaatggcca cgagcaatcc caccgccgtg gctttcagcg
     9001 ccagactgag gccgaccatg atgctgctca ccgccatggc gccggaggtc cccatcgtgt
     9061 gaaacgtcag catgatgccc agcaccgtcc ccagcaaacc gatatagggt gcattcgccg
     9121 caatggtgcc gatgaccacc aatcgcttcg tcaacgccat ctcaaacgtg tccagcgtga
     9181 caaatgccga cggatcgacc cgtctgtaga agtgccatcg ctcgatcgcg atgattagcg
     9241 cccacacgcc gagcagaccc aagagaccga tcactccata ctccactcca aatttcagag
     9301 actccatggc tttccgtcct cactgttcct tatcgatgac gtccatcacg cgcggccggg
     9361 aaaaggcctg cgggcctcac attggcggcg ctacgttacg atggcctgcg cagaccgtct
     9421 actgacgcgt gctcatggct gctacagttc ggagagaccc acggagaggg aaagttggga
     9481 taaagctacg atgactagac aggggacaat gtgtgcagga cagcagccgg gctcagtcga
     9541 ggtgttgggt aaggaaccgt tccatctcag agaatacgtg gaggcggtcc tcctcccggc
     9601 gcagggcttc atcagcgaga ggcaattcca cgtatcgagc gttcgtggca ttcgccgctt
     9661 tgagcgcatc ggccagattg cggctctgtg aaaccggcac ggaacgatcc atggacccgt
     9721 gcatcaagag cagaggagtg cgcatccctt gcgcatgagc cacgggcgat gtctcacgaa
     9781 gccggtcacg gtcgttccac caggaaccga tccgtgcctc gaccatcggt ttctgattca
     9841 ggtacccccg gccatctgac accagttgcg gcaaatccgt caccccgccc agactgaccg
     9901 cacaacgata gagatccggc gtcttcacga cgcccatcaa cgcggcgtaa cccccatagc
     9961 ccgacccaac gatgcagatg cgaccggcat tggcgatgcc ggcacgaaca gcatattgca
    10021 ccacatcggt caggtcgttc tgcatctcca gaccccagcg ttgaaatccg gcgcggagca
    10081 cgtcatcccc atagccctca gtgccccgga agcgcggctc caacactgcc cagccgcggc
    10141 tgacgaacca ctgtgtccaa taattaaacg cgcctggcat gcgtgcagca ggtcctccgt
    10201 gaggaaacac gatcatcgga aggtggcgcg ggtcccgatc cttgggaacg gtcagaaaga
    10261 cttgcagctc cttgccgtcc cgcgccgtca cataggtcgt ggtgggagca gccagcgcgg
    10321 ccgtctcgag atcgggatac gacctgccga gcagcaccat ccgtccgtcc tgctcgtcga
    10381 agatcgacca gcgcggaggc tgcgctgcac cgctcgactt gacgacatgg aggcgcccgt
    10441 cggcgctgct gctgtggatg acattgacgg ttccgggaat cgcccgatcg atacgcgcct
    10501 gcaggcgttg cgcgtcgaag tcccagaaga gcacccgttc atccgccgcg ctgtaccgca
    10561 cgccgacgac cttcttgcgt ccaggcgcat agaccagttc gccattgaga tcaaacttcg
    10621 gatcggcgac cacgagcaca cgatctgccg cacggtccat cacgttgagc ttaaaaatcg
    10681 ccgtcttgcc ccgatgttga tccctcacat acaaccaggc gggatcggca tcgaaggcca
    10741 ggggaacaag tccggtttcc ttggccaggt catagtcagc cagttcgcgc cagagtgtgg
    10801 actccggttg cctaacgatg gcatgcaccg cggtctggaa ttggcccacc cccgaccgca
    10861 cggctcctgt gcggtcggca atccaatggg aaatggcgtt gcggtccggt ttcgaactgg
    10921 gattcgcctg aaccaactgc cgctcaccgg aatagacatc caccttgtag acatccggag
    10981 cattgggatg ttccagatcc agcgcaagca gcacgtgccg cggatcgccg ggaatcatcc
    11041 cgaccagctg atattcgaac tgaggaacat gtttctgtcc aaacaaggag gagaagccac
    11101 cctgcttgag taaattggcg gtctgctccg tgccatcacg gttcaccgcc atcaatcgag
    11161 tctcaaccgt gtctgtcgcc tccggggtag cggcgaaccg catgctcacc agcagacgct
    11221 cgtcattgat ccaacgaaac caggtgatga tgtattcgcg attgtccgta gaaacgaccg
    11281 ggtgcgcatc ctgtcccgtg acggtgtggg tttccagaaa cgtctgtcca tcatgatggc
    11341 ggagaacggc cagatgccgg ccggaagggg acagttgaat cgattcaatt cggggcagtg
    11401 aggcaaaggc cgcggtaggc agaggagcgg agccgggctc ggcgtagacc ggtaccatgc
    11461 tccagacaag tagcagaaca gtgacaagtc cgtagatggc cggcacggtc tatccctaca
    11521 gttaccagg
//
LOCUS       DAOQEW010000118        11504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1681_length_11504_cov_8.966373, whole genome shotgun sequence.
ACCESSION   DAOQEW010000118 DAOQEW010000000
VERSION     DAOQEW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_11504_cov_8.966373"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..681
                     /gene="rpmH"
                     /locus_tag="PLS35_12790"
     CDS             544..681
                     /gene="rpmH"
                     /locus_tag="PLS35_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNI19591.1"
                     /translation="MSFTFKQPSNLKRKREHGFRKRMSTKNGRKVIARRRAKGRARLT
                     V"
     gene            861..1232
                     /gene="rnpA"
                     /locus_tag="PLS35_12795"
     CDS             861..1232
                     /gene="rnpA"
                     /locus_tag="PLS35_12795"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNI19592.1"
                     /translation="MTAGQASASFFLKRSGDIQLIKKNGRRVSTGLFNLQICPGTMPQ
                     DTMMGIVIGRRFGTAVRRNRAKRLFRELGRAVRPTLSAGHAFLVFPKRQCLNVPFVEL
                     QSVWRSTLQRQRLVRVGDPSS"
     gene            1175..1438
                     /gene="yidD"
                     /locus_tag="PLS35_12800"
     CDS             1175..1438
                     /gene="yidD"
                     /locus_tag="PLS35_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNI19593.1"
                     /translation="MAQHIAKTAVGSCWGSLVVRYFCIGLLSVYRACVSPMLGPACRF
                     HPTCSVYAQDAIERYGLLKGVAMAGWRLLKCHPFHPGGVDPVR"
     gene            1484..3229
                     /gene="yidC"
                     /locus_tag="PLS35_12805"
     CDS             1484..3229
                     /gene="yidC"
                     /locus_tag="PLS35_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNI19594.1"
                     /translation="MEKRVIVFLIVSLAVIIGYDYLLKGMGLLPPSEPVQVATNTQVA
                     ERQSAGSENASAQSVSPASSTQTSSPSAPGASDGGTGTVKEEQTIEVDTDLFRAKFSN
                     RGGVLKSWELKRYTTSTDQGPVPVQLVYAGGRFKGPLSLVTTDQSVTSDLGSALYQVT
                     QDISHLDSAHPTGHITFRYHDTQRNMDVEKELTFHHGSYVVDIAVRTQGLATPVDVTL
                     GTNFGIVEWGEGFIGLMGSASLVDDKVLKETPDGEAERKGDVKWSAIQDKYFLSVLMP
                     KKAMSALAKKEGDKLVSAGVRFPAPTPGSTMTMQLYAGPKEYDTLKGLNVGLEDTIDF
                     GWFVFGSWALVKAVAKPIFYVLRFLYEFTHNYGVTIILLTMLIKLMFVPLQYKSYKSM
                     KQMQVIQPKVLAIQNKFKDDRERLNKELIKLYKDHRVNPVGGCLPMVLQMPVFVALFN
                     ILYMTIDLRQAPFMLWIKDLSVQDPYYVLPIIMGATMVIQQKITPTTMDPTQAKIMLF
                     LPVFMTFLFVNFPAGLVLYWLTNNTLTITQQVVTERLFGKKWQVAPEGADAAAIEDSK
                     EQKSNGKRRNASATE"
     gene            3251..4681
                     /gene="mnmE"
                     /locus_tag="PLS35_12810"
     CDS             3251..4681
                     /gene="mnmE"
                     /locus_tag="PLS35_12810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNI19595.1"
                     /translation="MLGALDDTICAIATPPGEGGIGILRISGFHALDVASHVVRLRSG
                     NALQDLPTHRLALADICADNTDVPAAGTKRTGALRFIDEALVVVMKGPHSFTGEDVVE
                     VQCHGGPVILDQLCRGLISAGARLAAPGEFTKRAFLNGRLDLAQAEAVLDTIRAKTAR
                     SLTLAQSQRRGELSRHVEEIRSELVVALAHIEAALDFAEEDISFVRQDELLRLLDVTL
                     QKLRRLLQSGRDGRIWREGAVVAILGRPNVGKSSLMNALLRSDRAIVTAIPGTTRDLL
                     EESLSIDGIPVRLLDTAGIRVTDDPVEVEGIRRSRLAWTDADLALILLDGSQSLSESD
                     RALLSEPDSTRALLIINKCDLPGQLSREDLDRACPGHSGILEISATLSIGLDELRETI
                     RGRLMPGMWESNEGVLVTNLRHTVAIERALQGVDQARQSVEAGRAGELIAMDLRIAAD
                     ALGEITGVITTDEILDRVFAEFCIGK"
     gene            4695..6575
                     /gene="mnmG"
                     /locus_tag="PLS35_12815"
     CDS             4695..6575
                     /gene="mnmG"
                     /locus_tag="PLS35_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNI19596.1"
                     /translation="MSEQCDVIVVGGGHAGCEAALAAARMGARTLLLTMDPDRIAQMS
                     CNPAVGGIAKGHLVKEIDALGGEMGRNTDQAGIQFRMINTSKGPAVRALRAQCDKKIY
                     RQVMQDTLRAQPCLRIRGGTVDRILTHAGAVVGIVTDSGQHIEARAVVLTSGTFLKGL
                     IHIGLSHFPAGRAGEASAEHLSDCMRDFGFEVGRLKTGTPPRLDRDTIDFSVMELQPG
                     DDPPPPFSYRTNRIPLPQVPCHLTHTNTQTHDIIAKNIDRSPLFSGVIDGIGPRYCPS
                     IEDKVVRFADKDRHQIFIEPEGLDTNEFYPNGISTSLPVDVQAAILRTIPGLKQAKIL
                     KPGYAVEYDYFPPRQLHNSLETKLVQGLYHAGQINGTSGYEEAGAQGIMAGINAALKL
                     RGDAPLVLDRSQAYIGVLIDDLITKDAREPYRMFTSRAEYRLLLRHDNADLRLMDLGH
                     RIGLIPDSVHHKFQEKRLAIEREVQRLKDTRPKLTPDVRRLLEEFEIGPVTPTQSLAE
                     ILRRQDLTYGKISSVFGGCEIEDADIMDAVQLEVKYEGYIKRQQQQIQRSQKFEHRAI
                     PESFDYDDVSGFSAEVREKFKRVRPASVGQASRISGVTPAAISLLLVAIERHKRQPLT
                     AE"
     gene            6665..7309
                     /gene="rsmG"
                     /locus_tag="PLS35_12820"
     CDS             6665..7309
                     /gene="rsmG"
                     /locus_tag="PLS35_12820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNI19597.1"
                     /translation="MEHDASQFEVSFRRFAAELGIPVDSSCLPFFSTYLSELKKWNKA
                     INLTAIHDDQEVLVKHFIDSVAGVKVIENMDKCELLDIGAGAGFPGLPLKFVLPQIEL
                     ELLEPSEKKGAFLRYVAGSLGLRHVTVVPSKLEQYVRRPTRRLFDYIVVRAFKVDESG
                     QMVADLLKPAGKVILYRAERVPRGFRLGGLALVQEVEYELPSGYGHRVLSVFSK"
     gene            7381..8157
                     /locus_tag="PLS35_12825"
     CDS             7381..8157
                     /locus_tag="PLS35_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNI19598.1"
                     /translation="MARIIAVANQKGGVGKTTTSVNLAAALAIEGGSVLLVDIDPQGN
                     ATSGLGVDAMSLGKTIYNALIVKESITDLAMPTAVNGLSLVPANSHLAGAEVELVNME
                     GREQRLKEALAEVADRYDTILLDCPPALGLLTINAMVAAHSVLIPVQCEYYAMEGLGR
                     LMESIERMRQSLNPSLEIEGIVLTMYDARNSLARQVVEQIRGHFGEAVYQTMIPRNVT
                     LAEAPSYGRPALLYNMASSGAQAYLSLAKEFVVHGEKSPR"
     gene            8135..8986
                     /locus_tag="PLS35_12830"
     CDS             8135..8986
                     /locus_tag="PLS35_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNI19599.1"
                     /translation="MEKKALGRGLDALLPTGRNTADPERGDVQELRLEAVVPNRFQPR
                     QQFSEVELAELTASLKQNGLLQPILVRRKGDGIYELIAGERRLRAAKLAGMQKIPALI
                     RNVSDQESMVLALVENLQRDDLNPMETARAYQRMLNEFSLTQEAIAQKVACDRSSVAN
                     MLRLTSLPAEVQHMIETDQLSTGHAKVILGLMTPAAQISLATQIVSGQLSVREAERLV
                     QGHAEAKKAGKRPVRAPLRSDLEERLQKRLGTRVDVQKGRRGGKIIIHYFSPEELDGV
                     VEQLLNG"
     gene            9125..9601
                     /locus_tag="PLS35_12835"
     CDS             9125..9601
                     /locus_tag="PLS35_12835"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNI19600.1"
                     /translation="MWIKDKPEGKRQAGQSEGSEMENLESTAPREGNEEINAFVGKGV
                     SFKGVISYNGTVRIDGNLDGEIHTEGVLLVGEDAVLTAKITAGTVVCKGKITGDISAR
                     EKVKLRAPAVVNAGVKAPLLAMEEGVVFNGTLEMSQTGTREPQGTSRGQGVKLVNG"
     gene            9727..10182
                     /locus_tag="PLS35_12840"
     CDS             9727..10182
                     /locus_tag="PLS35_12840"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNI19601.1"
                     /translation="MWGETKKQSVAESDKFTFLGKGTSFKGIVTFDGTVRIDGRVEGE
                     VHTGGAVIVGESAVIKGVIAAGSVEISGRVKGSITAQQKVEIQKPGILIGDIQSPVIM
                     IEEGAHFHGMSNMGAEKWSEDEASEFGSPQDPGVRDLVAHRGKVKTQEP"
     gene            10215..11297
                     /gene="mnmA"
                     /locus_tag="PLS35_12845"
     CDS             10215..11297
                     /gene="mnmA"
                     /locus_tag="PLS35_12845"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNI19602.1"
                     /translation="MTRQTVLLGMSGGVDSSVAASLLVQQGYDVHGVTLQVWEHEDEH
                     VAVSKRWEERGCCKIGIAKFVAQRLRIPFEVVDRREIFQQGVIDDFVAGYAAGTTPNP
                     CVRCNERVKLRSLYALAQERGMDYVATGHYARVQQAGGQWSLHRALDARKDQSYFLYR
                     IQADWLPRLLFPVGEMQKSDVWKEAETLGLPVEELKESQEICFVSQGDYRTFLEQERP
                     ELKQPGAFVGVDGEVLGRHEGIAFYTPGQRRGLGIAVGQRLYVQKVVPESGEVVLCAE
                     EQLVQSDCQVGDISLFDRSIGRQAVDVDVKIRYATPPAAATLLPSTNGTVQIRFHQPQ
                     RALSPGQSAVFYQGDRVLGGGIIQRT"
BASE COUNT         2456 a         2932 c         3508 g         2608 t
ORIGIN      
        1 acgagggtaa cgcgagtggc gatgcggcag atgtaacgtg agcggctcat cattgttgca
       61 ttctagcgtg cggatggctg tcccgcgagt tcttcccgcc ttttgtccct aactggacgg
      121 tagtgttacc gtggtgcctt ctgatacgag ggctccggcc tatccgcggt ccttcatgga
      181 ttccgcgtga aggtcgatga ttccatcatt ccaacatcct cctacgcaat gtaagtgcca
      241 gaaagtgcat tcgtgcgaag accattgaat gccttgggag aacaataagt tacggtgcag
      301 tattccgttg tcttactggc tcgccgctgt ggcatctttg ccgcaatcgg gcaagagtgc
      361 ctcaaagaag tggcgattga taaggcgtgt gcgcatcgga gaggccgcgc gcttcatgca
      421 gtctgcattt caggggacga tttcatcgcg gagaggcgat ggtagattga ttgacaccct
      481 cctgtgacgc ccctataatg cggcctcttc attgaaaaat gatctctcga gaaggagtgc
      541 gcaatgtcgt tcacttttaa acagccctcg aatctcaagc gcaagcggga acatggattt
      601 cggaagcgca tgagcacaaa gaacggccgc aaggtcatcg ctcgtcgccg agccaagggc
      661 cgcgctcgcc tcaccgtctg attctttcct gccgctaggc aatctgcatg taggcaaaca
      721 tccaggtggt gggtagtcac cgggggtgga tactgtcgtc ttagcctggt cgtggaagaa
      781 catgggcgat acagcgggcg atatgcggct tcaaacgatt gaagcccggt cgaacctggt
      841 aacccgcaga caggtcggtt atgacggcag ggcaggcgtc tgcgtcgttt ttcttgaagc
      901 gtagcgggga tatccagctc atcaagaaga atgggcggcg tgtttcaaca ggattgttca
      961 atctccagat ctgtccgggt acgatgcccc aagacaccat gatggggatt gtcatcggac
     1021 ggcgattcgg gacggcggtg cggcggaaca gggcgaagcg cctgttccgc gaattgggcc
     1081 gtgcagtgcg gccgacgttg agtgcggggc acgcatttct tgttttcccg aagcgacaat
     1141 gcctgaacgt gccgtttgtg gagctgcaaa gcgtatggcg cagcacattg caaagacagc
     1201 ggttggttcg tgttggggat ccctcgtcgt gaggtatttc tgcatcggcc tgctgtcggt
     1261 ctaccgggcc tgtgtgtcgc cgatgcttgg tcccgcatgc cgcttccatc cgacctgttc
     1321 cgtctatgcg caagacgcca ttgaacgata tggattgctc aagggggtag cgatggctgg
     1381 ttggcgcctg ctgaaatgtc accctttcca tcctggcggc gtagacccgg tacggtgatc
     1441 tttgtgacga caacccctca ggtgttattc ggtacataaa ttcatggaaa agcgcgtcat
     1501 tgttttcctc atcgtctctc tggccgtcat tatcggctat gactacctcc tgaagggcat
     1561 ggggttgttg cctccgtctg agcctgtgca ggtcgcgacg aatacccagg tcgcggaacg
     1621 acagtctgca gggtccgaga atgcctctgc ccagtccgtg agtccggcat cttccactca
     1681 gacaagttct ccttccgcac ctggggcatc agacggtggc acgggaacgg tcaaggaaga
     1741 gcagacgatc gaggtggata cggacttgtt tcgggctaag ttctccaacc gcggcggagt
     1801 gctcaagtcc tgggagctga agcgatatac cacgtcaacc gatcagggcc ccgtgccggt
     1861 acagctcgtg tacgccgggg gacgcttcaa gggtcctctg agtctggtca ctaccgatca
     1921 atctgtgacg tcagatctcg gatctgccct ctaccaggtg acccaagaca tttctcacct
     1981 cgatagcgcc catcccaccg gacacatcac attccgatat cacgacacgc agcggaatat
     2041 ggatgttgag aaggagttga cgttccatca cggatcgtac gtggtggata tcgcggttcg
     2101 gacgcaaggc ctggccacgc ccgtggatgt cactctcggg accaattttg gcatcgtcga
     2161 atggggcgaa ggatttattg gcctgatggg ctcagcctcc ctggttgatg acaaggtctt
     2221 gaaggaaacc ccggacggtg aagcggagcg taagggagat gtgaagtggt ccgccattca
     2281 ggacaagtat ttcctgagcg tcctgatgcc caagaaggcg atgtctgcgc tggcgaagaa
     2341 agagggcgat aagctggtgt ccgccggagt caggtttcct gcgccgactc ccggatcgac
     2401 aatgacgatg cagctgtatg ccggaccgaa agagtacgac accctgaagg gcttaaatgt
     2461 cggcttggaa gatacgatcg actttggttg gtttgtcttt ggcagttggg cactggtgaa
     2521 agcagtcgcc aagcccatct tttatgtcct gcgctttctc tacgagttta cgcacaacta
     2581 tggtgtcacg atcattctct tgacgatgct gatcaagctg atgttcgtgc cgctgcagta
     2641 caaaagctac aagtcgatga agcagatgca ggtcattcag ccgaaggttc tggccattca
     2701 aaataagttc aaggatgatc gcgagcggct gaacaaggag ttgatcaagc tctacaagga
     2761 tcatcgggtg aaccccgtcg ggggctgtct gccgatggtc ctgcaaatgc cggtcttcgt
     2821 cgcgctgttt aatattctct acatgacgat cgacctcagg caggcgccgt tcatgctctg
     2881 gatcaaagat ctttccgtgc aggatcccta ttacgttttg cccatcatca tgggcgccac
     2941 catggtcatt cagcaaaaga ttacaccgac caccatggac ccgacgcaag caaagatcat
     3001 gttgttcctc ccagtgttca tgacgttcct gttcgtaaac ttcccggcag ggttggtgct
     3061 gtattggctg acgaacaaca cgctcacgat tacgcagcag gttgtgacgg agcggttatt
     3121 tgggaagaag tggcaggtgg cgcccgaggg cgctgacgcc gctgccatcg aagattcgaa
     3181 agaacagaaa agtaacggta aacggcgaaa cgcctccgcg actgaataac accacctcac
     3241 tgggtgcgtc atgcttggcg cgctagacga taccatctgt gcgattgcca cccctcccgg
     3301 ggagggcggc atcggcatac tccgcatcag tggctttcac gcccttgacg tcgcgtctca
     3361 cgttgtacgt ctccggtcgg gtaacgcctt gcaagatctt cccacgcatc ggctcgccct
     3421 cgccgacatc tgcgctgaca atacggacgt acctgccgct ggtaccaagc gcacgggggc
     3481 gttgcgtttt attgatgaag cgctcgtcgt cgtgatgaaa gggccccatt ccttcaccgg
     3541 ggaagatgta gtcgaagtgc aatgccacgg tggtcccgtc attctggatc agctctgtcg
     3601 gggattaatc tcagcgggag ccaggcttgc cgcaccgggt gaatttacca agcgcgcgtt
     3661 tctcaatggg cgtctcgatc tggcgcaggc agaggcggtg ctcgatacca ttcgcgcgaa
     3721 gaccgcacgc agcctcaccc tcgcccagtc gcagcgccgg ggcgagttat cccggcacgt
     3781 ggaggagatc cgatctgaat tagttgtggc tctggcacac atcgaagccg ctttggattt
     3841 tgccgaggag gatatttcgt ttgttcgtca ggacgaattg ttgcgcctgc ttgatgtgac
     3901 cctgcagaag ctgcggcggt tgcttcagtc gggacgtgat ggtcgcattt ggagggaagg
     3961 cgcagtcgtg gcgattcttg gacgtcccaa tgtcgggaag tccagtttga tgaacgcctt
     4021 attgcgaagt gatcgcgcca tcgtcacggc tatccccggt acgacccgag atctcttgga
     4081 ggaatcgctc agcattgatg gcattcccgt gcgattgttg gatacggcgg gcatacgcgt
     4141 gacggacgat cccgtagaag tcgaagggat tcgccggagc aggctggctt ggacagatgc
     4201 cgatctcgca cttatcctcc tggatggttc ccagtccttg tccgagagcg atcgggcgct
     4261 cctttcagag cctgattcga cgcgtgcgtt gctcatcatc aataaatgcg atctccctgg
     4321 ccaattgtcg agggaggatc ttgatcgcgc gtgtcctggc catagcggta ttctcgaaat
     4381 ttccgcgacg ttgtcgatcg ggctggacga actgcgcgaa acgattcgtg ggcggctgat
     4441 gccggggatg tgggagtcga atgaaggtgt gctggtgacc aatcttcgcc acactgtcgc
     4501 cattgagcgg gctttgcaag gggttgatca ggccaggcag tccgtcgagg ctggccgtgc
     4561 aggggaattg atcgccatgg atctccgcat tgccgcagat gccctaggag aaattacggg
     4621 cgtcattacg accgacgaaa tcctcgatcg cgtatttgca gagttttgta tcgggaaatg
     4681 aaagggtgtc gggcatgagc gagcaatgtg acgtcatcgt ggtaggagga ggacacgccg
     4741 gctgtgaggc ggccttggct gcggctcgta tgggcgcgcg cactctgttg ctgaccatgg
     4801 atcctgaccg tatcgcccaa atgtcttgta atccggcggt cggtggaatc gccaaggggc
     4861 atctcgtcaa agagattgat gccttgggcg gcgaaatggg ccggaacacg gatcaggcag
     4921 gcatccaatt ccgtatgatc aacacgagta aggggccggc ggttcgcgcg ttgcgcgcgc
     4981 agtgcgataa aaagatttac cgccaagtga tgcaggatac gcttcgcgca cagccgtgcc
     5041 ttcgcatcag aggcggaacg gtcgatcgta tcctgacaca tgccggggcc gtggtcggca
     5101 ttgtgaccga ttcaggtcag cacattgagg ctcgggcggt ggtgctcacc tcgggcacct
     5161 ttttgaaagg tctcattcac atcggtctga gtcattttcc ggcgggacga gccggagagg
     5221 cttcagctga acatctttca gattgtatgc gcgatttcgg gtttgaggtg ggccgtctga
     5281 aaacgggcac tccgcctcgg ttggatcgcg ataccatcga cttttcggtc atggaacttc
     5341 agcccggtga cgatccgccg ccgccctttt cttaccgcac gaatcgaatt ccccttccgc
     5401 aggtgccgtg tcatttgacc catacgaaca cgcaaacaca tgatattatt gctaaaaata
     5461 tagacaggtc gccattgttc agcggcgtca tcgatggtat cggtccacgc tattgcccgt
     5521 cgatcgagga taaggtcgtg agatttgccg acaaggaccg gcaccagatt ttcattgagc
     5581 ccgaggggct cgataccaat gagttctacc cgaatggcat ttcgacgagt ttgccggtgg
     5641 atgtgcaagc agccatctta cgaactattc ccggcttgaa gcaggccaaa atcttaaagc
     5701 ctgggtatgc ggtggagtat gattatttcc cgccgcggca gctccataat tcattggaga
     5761 cgaagcttgt gcaggggctt taccatgccg gtcagatcaa tggcacctca ggctacgaag
     5821 aggccggcgc tcaaggcatc atggctggta tcaatgccgc gttgaagttg agaggagatg
     5881 cgccactcgt actcgaccga tctcaagcct acatcggtgt attgattgat gatttgatca
     5941 cgaaagacgc gagagaaccc taccggatgt tcacctcacg cgcggaatac cgcttgctgc
     6001 tgcggcacga caatgcggat cttcgcctga tggatctcgg gcaccggatc gggctcatcc
     6061 cagatagcgt ccatcataaa tttcaagaga agcgtttggc catcgagcgc gaagtgcagc
     6121 gactgaagga tacgaggccg aagttaacgc cggacgtcag gaggctgctt gaagaattcg
     6181 agattgggcc ggtgacgccg acgcaatcgt tggccgagat tctcaggcgg caggatctga
     6241 cctatgggaa gatttcttcg gtgttcggcg ggtgcgagat tgaagatgct gatattatgg
     6301 atgcggttca gctagaggta aagtacgagg gctatatcaa gcgccaacag cagcaaatcc
     6361 agcgatcaca aaagttcgag cacagggcga tcccagagag ctttgactat gacgatgtgt
     6421 ctggattttc tgccgaggta agggaaaagt tcaaacgtgt aaggcccgca tctgttgggc
     6481 aggcatcacg aatttccgga gtcacgcctg ccgcaatttc gctgttattg gttgcgatcg
     6541 agcgccacaa gcgtcagccc ctcactgctg aatgatttcg gtattccgct ctctttctgg
     6601 ccagccttcc taatttgcag aaacattctt gacccttggt attggtttcg cgtaattgtt
     6661 ccacgtggaa cacgacgcca gccagtttga agtatctttc cgaagattcg ccgcagagct
     6721 cggcatccct gtagatagtt cttgtctgcc gtttttttct acctacctct cagagctcaa
     6781 aaaatggaat aaggcgatca atctgactgc catccatgat gatcaggaag tcctggtgaa
     6841 gcatttcatc gattctgtgg ctggggtaaa agtcattgaa aatatggaca aatgtgagct
     6901 tctagatatt ggggctgggg ctggatttcc aggtctgccg cttaaatttg tgctgcccca
     6961 aatagagtta gaactgcttg agccgagcga gaagaagggg gcctttctga ggtatgtcgc
     7021 cgggtccctc gggttgaggc atgtcactgt tgtcccatcc aagttggagc agtacgttcg
     7081 ccgaccgact cggcgactgt tcgattacat cgtagtgcga gctttcaagg tagacgagag
     7141 tggccagatg gtggccgatc tcctcaaacc agccggaaaa gtgattttat atcgagccga
     7201 gcgggttccg agaggtttta gactgggtgg ccttgctttg gtgcaggaag tcgagtatga
     7261 gcttccatcc ggatatggac atcgcgttct ttccgtattc tcgaaataga aggccagctc
     7321 ggatgttcca cgtggaacat cgcagtcggt tcagtgtgct gctagaagat tggggatgac
     7381 atggcgagaa ttattgctgt cgcaaatcag aagggtggag tgggcaaaac caccacctcg
     7441 gtgaatttgg ccgcggcctt ggcaattgag gggggatccg tgctcctcgt ggatattgat
     7501 cctcagggta atgccaccag tggcctcggt gtcgacgcca tgtcgcttgg aaagaccatc
     7561 tacaatgcct tgattgtgaa agaaagtatt accgatctgg cgatgcccac tgcggttaac
     7621 ggcttgtcgt tagtgcctgc aaattcacat ctagcgggcg ctgaggtcga actcgtgaat
     7681 atggaaggta gggagcagcg cctcaaggag gccttagccg aggtggctga tcgatacgac
     7741 accattttgt tggactgccc accggccctt ggtcttctta ccatcaatgc catggtcgct
     7801 gcgcactccg ttctgattcc ggtgcaatgc gaatattacg cgatggaagg tcttgggcga
     7861 ctcatggaaa gcattgagcg catgagacag tcattaaatc cgagcttaga aattgagggc
     7921 attgtgctga cgatgtatga cgcacgtaat tctttggcgc gccaagtcgt cgagcagatt
     7981 cgcggccact ttggcgaagc ggtgtatcaa accatgattc cgcgtaacgt cacgctggcg
     8041 gaggccccca gttacggtcg tccggcgttg ctctacaaca tggcatcatc cggtgctcaa
     8101 gcctatcttt ccctagccaa ggagtttgtg gtccatggag aaaaaagccc tcggtagagg
     8161 tctcgacgct ctgttgccga ctggccgaaa tacggccgat cccgagcgag gcgatgttca
     8221 ggagttgcgc ttggaagcgg ttgtcccgaa tcgttttcag ccaaggcagc agttctccga
     8281 ggtcgagctg gctgaactga cagcgtcgct caagcaaaat ggcctcctgc agcccatttt
     8341 agttcgccga aagggcgacg gcatctatga attgatcgcg ggagagcggc ggctgagagc
     8401 tgctaagttg gcgggaatgc agaagattcc tgctcttatc cgaaatgtgt cggaccagga
     8461 atcgatggtg ctcgcgctgg tggaaaacct tcagcgtgat gaccttaatc cgatggagac
     8521 cgcccgggcg tatcagcgca tgttgaatga attcagcctc acgcaggagg cgattgcgca
     8581 aaaggtcgcc tgcgatcggt catcagtggc gaacatgctg cgcctgacct cgttgcccgc
     8641 cgaagttcag catatgatcg aaacagatca gttgtcgaca ggccatgcga aggtcatcct
     8701 cggtctgatg accccggctg cgcaaattag tttggcgacc cagatcgtga gcgggcagtt
     8761 gtcggtgcga gaagcggagc gattggttca agggcacgca gaggctaaaa aagcagggaa
     8821 gcgtccggtg cgggcgccgt tgcgatcaga tctcgaggag cggttgcaga aacggctcgg
     8881 caccagagtc gatgttcaga aggggcgccg tggcggaaag atcatcattc attatttttc
     8941 tcctgaagaa ctggacggcg tggttgagca gttgctgaac ggatagcgga ggcattttca
     9001 gggcagattt tacggtttga ccggagtaca atgccgacgg aatttgtctc gtcattgagg
     9061 tggacggtga agaattcccg ctccgccggt cagacgtgtg gttatttgtt tggaggtatg
     9121 cactatgtgg attaaggaca aaccagaagg aaagcgccag gcaggacaaa gcgagggaag
     9181 tgaaatggaa aatttggaat cgacagcacc ccgtgaaggg aatgaagaga tcaatgcctt
     9241 tgtcgggaag ggtgtcagct ttaagggcgt catttcctac aacggcaccg tgcgtatcga
     9301 tggcaatctg gatggcgaaa ttcacaccga aggtgttctg ttggtcggtg aggacgcggt
     9361 gctgaccgcg aagatcacgg ccggcacggt ggtgtgtaag ggaaagatca ccggcgacat
     9421 cagtgcgcgt gagaaagtga agttgcgcgc gcctgccgtg gtgaacgcgg gtgtaaaggc
     9481 tccgttgctc gccatggaag agggcgtggt gttcaatggt acgttggaga tgagccagac
     9541 ggggacccgc gaaccgcaag gtacgtcacg cggacagggc gtaaagctcg tcaacggtta
     9601 gtccggctcg atcacgaatc ataggacagc gtgcgaccgg ccttatgccg gatcggcatc
     9661 aatagagttc cattcagtcc gcgccacggg cggtcaccgt cgtggggtgc gtgaaggagg
     9721 acgctgatgt ggggtgagac caaaaagcag tccgtcgcgg aatccgacaa gtttaccttt
     9781 ctcgggaagg gaacaagttt taagggcatc gtcacctttg acggcaccgt ccgtatcgat
     9841 ggccgcgtcg aaggcgaagt ccacacaggt ggtgcggtga tcgtcgggga aagcgccgtg
     9901 attaagggcg tgattgccgc cggatccgtt gaaatcagcg ggcgtgtgaa gggatcgatt
     9961 actgcgcagc agaaggtgga aattcagaag cccgggattc tcatcgggga catccaaagc
    10021 cccgtcatta tgatcgaaga aggtgcccat tttcacggca tgagcaacat gggcgctgag
    10081 aagtggtcgg aggacgaggc ttccgaattc ggttctccgc aggatccggg cgtgcgtgac
    10141 cttgtggccc atcgcggtaa agtcaaaacc caagagccgt aagtgcgcgg ctccttgttc
    10201 ccccggtatt ttttatgaca cgtcagacgg tgctgctagg gatgagcggc ggagtcgata
    10261 gctccgtcgc tgcatcgttg ctcgtccagc aagggtatga tgtccatggc gtcacgctcc
    10321 aggtctggga gcacgaagac gagcatgtcg cggtgtctaa gcgatgggag gagcggggct
    10381 gctgcaagat cgggattgcc aagttcgtcg cgcaacgcct acgtattcct tttgaggtcg
    10441 tcgatcgccg ggaaattttt cagcagggtg tcatcgatga ctttgttgcc ggatatgccg
    10501 caggaaccac ccccaatccc tgcgtccggt gcaatgagcg ggtcaagctt cgttctctct
    10561 atgccctggc gcaggagcgg ggtatggact acgtggcgac ggggcactac gcgcgtgtcc
    10621 agcaggcggg cggacaatgg tcgttgcatc gggcactcga tgcgcgcaag gaccagagtt
    10681 attttttgta tcggatccag gccgactggc tgccgcgcct cttgtttccc gtcggagaga
    10741 tgcaaaaaag cgacgtgtgg aaagaggcgg aaaccctcgg attgcccgtc gaggagctga
    10801 aggagagcca ggagatttgt tttgtgagcc aaggcgacta tcggaccttc ctcgagcagg
    10861 aacggccgga actcaagcag ccgggagctt ttgtgggtgt cgatggtgaa gtgttggggc
    10921 gacacgaagg gatcgctttt tacacgccgg gccagcggcg cggacttggc attgcggttg
    10981 ggcagagatt gtacgtgcag aaagtcgtcc ccgagtcagg cgaagtagtg ttgtgcgcgg
    11041 aagaacagct cgtgcagtcc gattgtcagg tcggggatat cagtttgttc gataggagca
    11101 tcggtcgaca ggccgtcgat gtggatgtga aaatccggta tgccactccg ccggccgccg
    11161 cgacgctgtt gccatcaacc aacgggaccg tgcagattcg gtttcatcag ccgcaacgag
    11221 cgctgagccc cggacagtcc gcggtcttct accaaggaga tcgtgtgctt ggcggcggca
    11281 tcattcagcg cacgtaaggc gtggcctgcc gttgttcgct tttcagcacg taaaaacaca
    11341 atcaatattt gcctgggatg ccttttgcct cggtcttttc cgaaccgctt tgacggtcct
    11401 cctgcgacca ttcctcctgc cagccatggg atcctccttg acagtacccg cgtttgaatg
    11461 ctaacttggc gcgctttttg tcgtgcccac cgcacgcaag cttc
//
LOCUS       DAOQEW010000119        11487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1683_length_11487_cov_7.686494, whole genome shotgun sequence.
ACCESSION   DAOQEW010000119 DAOQEW010000000
VERSION     DAOQEW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_11487_cov_7.686494"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..574
                     /gene="aroQ"
                     /locus_tag="PLS35_12850"
     CDS             77..574
                     /gene="aroQ"
                     /locus_tag="PLS35_12850"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNI19603.1"
                     /translation="MLRLLVLHGPNLNLLGTREPSVYGQMSLSDIDKSITRRSGELGI
                     AVQTKQSNSEGELVTWIQNARGHFDGIIINPAAYTHTSIAIRDAIAAVALPTVEVHLS
                     NIHQREEFRHHSFIAGVAIGQIAGFGPTGYLLALEALTVHLGAKRSSSRTRADVRKKP
                     AASRR"
     gene            600..1160
                     /gene="efp"
                     /locus_tag="PLS35_12855"
     CDS             600..1160
                     /gene="efp"
                     /locus_tag="PLS35_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNI19604.1"
                     /translation="MISTAEFRNGSPLMVEGQPFYIVEFQHVKPGKGGAFVRTKLKSY
                     LSGNVLDRTFRSGEKFDTPEIEECDMQFLYATNDSYTFMDTETYEQSTYEKSQLGSNA
                     DLLKENMIAKILIYEHKPITVVLPNFIELKVVDGEPGVRGDTASGGSKPVIVETGATI
                     KVPLYLEIGEVIRIDTRTREFVERVR"
     gene            1157..1735
                     /gene="accB"
                     /locus_tag="PLS35_12860"
     CDS             1157..1735
                     /gene="accB"
                     /locus_tag="PLS35_12860"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNI19605.1"
                     /translation="MSPSRKKTGKSRTAAKPIVLPSAFAPRTSAADQMILNPDQAAQI
                     QQLADLLKRNHLTELEIERSGMRIRIRHEPAVRATTTHTVEAVHTQSATSGTAPAAQA
                     RPSAETDGQVTITSPIVGTFYRSPSPDADPYVEEGDYVKKGQVLCIVEAMKLMNEIES
                     EADGRITKILAESTKPVEYGQPLFLIDPSATP"
     gene            1913..3262
                     /gene="accC"
                     /locus_tag="PLS35_12865"
     CDS             1913..3262
                     /gene="accC"
                     /locus_tag="PLS35_12865"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNI19606.1"
                     /translation="MFKKVLVANRGEIALRVIRACKELGIKTVAIHSEADAASLHVRA
                     ADEHVCVGPPEAALSYRNIPNVLSAAEVTGADAIHPGYGFLSENAHFAEVCESIGVKF
                     IGPTSENIALMGDKSKAREVVAKKGLPVTPGSPGELRSEQDAQEAAKNIGFPVIIKAS
                     AGGGGRGMRVVNKPEELTRAFQAAQAEAKSTFGHDGVYLERYFLEPRHIEVQIVADNR
                     GHVVHLGERDCSIQRRHQKLVEETPSPAIDERLRREIGRTAVEAVKAIRYCNVGTVEF
                     LLDKDRNFFFMEVNTRIQVEHPITEMVTGIDLVKEQIRIASGLPLSFKQPDIKLNGHS
                     FECRINAEDPEKFTPCPGQITKYSAPGGLGIRVDSAMEPNAVVVPYYDSLIAKLITHG
                     RDRQEAMARMRRALDEFVIEGIKTTIPLHRKIFNDPDFQKGHVSTTFLDRFLAGQSA"
     gene            3335..3949
                     /gene="thiE"
                     /locus_tag="PLS35_12870"
     CDS             3335..3949
                     /gene="thiE"
                     /locus_tag="PLS35_12870"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNI19607.1"
                     /translation="MLDPTVRPERPLTDVLRVAAASGIRLFQYRDKQASMKDAYRQAL
                     ALRQVATEVGATLIINDRCDLALAVKADGVHLGQDDLPLADARQILGPEAIIGLSTHN
                     AKQVREAVALQPDYIGFGPIFGTTTKTDHDPVVGLEGLRAARTLTTLPIFAIGGITSQ
                     HVTDLTAAGADGVAVISAILKAQDLEAAIRSFQPPPSTQARPSH"
     gene            complement(3897..4643)
                     /gene="bioD"
                     /locus_tag="PLS35_12875"
     CDS             complement(3897..4643)
                     /gene="bioD"
                     /locus_tag="PLS35_12875"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNI19608.1"
                     /translation="MIGTTGVFVTGTDTGVGKTLVAAALVNRLVRMGRSVGVMKPVET
                     GVDAGLSDRTDAARLMAAAQVTDSLDLVSPYRFPAPVAPLSAAAAQGRVIELGDIVGR
                     YEQLAARYPCVVVEGAGGLLVPTGSDWAMADLISRLRLPVLLVGRVGLGGINHALLTL
                     EALERRNIPVVALVLNETVAPRGSVEQEQAASTVALLRDRVSMPLIGPLPYQTRADCA
                     WPEVVENVAGSRPISDLAELVWKAAAGSSG"
     gene            complement(4667..5428)
                     /locus_tag="PLS35_12880"
     CDS             complement(4667..5428)
                     /locus_tag="PLS35_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI19609.1"
                     /translation="MQSEPQTSASSPRELRTVLVTGGSGGIGRALCLAFARAGYWVGV
                     HYGRHKEAAEETLRELSTDGGAGAIYQADIRSGLEIQAMIDAVLDRQDRLDVLVCNAG
                     VASSHLVLRCPEDEWLRIIDTNLTGTYRCLTAVAPLMIKQGGGSIIVVGSYAGLHGAS
                     GQAAYAASKAGLVGLVQTAAREWGPHNVRVNLVCPGWQPTALAGDAFPSTEQLSDHVL
                     GRLMNLEDVSRTICHLAQLPGMSGQIVNLDSRILY"
     gene            5535..6437
                     /locus_tag="PLS35_12885"
     CDS             5535..6437
                     /locus_tag="PLS35_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19610.1"
                     /translation="MVQNLFRSTLIAVGILGSAAILVGHDTASATEAGQAIAQSADYF
                     PDTIGTRWQYRGQISEGPLQTIENKYFSNVSSVTGTRTLKGGLVVTVFHDTNPGNHGP
                     SDSFYRRDAAGVVYYGSEPGTPLEKQLTPYQIIRFPMKVAQSFQQFNRTDIDFGSDID
                     GDGTNEKVDVEGLSTVLGQESITVPAGTYPDAVRVEAKMTLKIRLSSVDRTAVGTDVM
                     TAWFAKGVGLVKYIERQELAALKDDRGNITDITEELEEVSVKSAPGLQGRHESAPEGL
                     LADDTSNQKLFQVLFASGLRAYAR"
     gene            complement(6327..9308)
                     /locus_tag="PLS35_12890"
     CDS             complement(6327..9308)
                     /locus_tag="PLS35_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNI19611.1"
                     /translation="MTLDPALLEPRIRTIGEDLARRSSGLSPGLFDSRWWSQAAINLA
                     MKDPAFKAQLFRFIDVLPSLRDDAQVVRLAEEYFGDMSGHLFGAQWGLKALASTKLGA
                     ALSGKAIRHQVEQMATSFIAGASIEQAVPALRKLWDDGRAFSVDLLGEASVNEREADA
                     YRDRCLAALRILGDVCPAWSPSPLLEHDHLGPLPRVQLSIKISALYSQLDPIDPESSY
                     RAVAARLRPLLNVAGTLPASIIFDMEQAETKDLILSIFMRLFEEEPYRTFAHAGIALQ
                     AYRIDTYDDVQRLLAWTTRRQVPITIRLVKGAYWDSDAIRYRQRGWPMPLFEQKSQTD
                     VNYESLVRLLLEQSHLIRPAFGTHNLRSLAVIEAVAEALGLPPVAWEYQMIFGMAEPF
                     QHAMSDRGRRLRLYTPVGELLPGMAYLVRRLLENTSNESFLRKEYVESQPLSLLLSPP
                     DGAPSHPAYSSPVEHKSSPYTSPTEFVNEPVADFSRASARTAMADAIERRRKHLGQRL
                     DLSTLAAHLPTGPDLSTRNPSPPEQIVAVVQSYQPADVAALTKIAGAAEESWTRRPVA
                     DRVAVIRKAASLMRDQRWDLAAWEVFEEGKPWREADADVAEAIDFLEYYAGEMARLDP
                     PPRLGRYPGELNEVLWNSRGVTVVIAPWNFPLAIPTGMVAAALVAGNPVLFKPSERSS
                     AMGYQLARILLDAGVPKGLLQYIPGGPELGRELVVSAAVRTIAFTGSKDVGLGIMQAA
                     GVQQPGQRFVKRVIAEMGGKNAVIVDETADLDEAVTGVVQSFSGYAGQKCSACSRVIV
                     VDSIHDAFLERLADAVMSLRVGPADDPATQVGPVIDARAKQRILDYLEIGRREGRVLV
                     DRSAEGPGYSVGPVVIADIEPVHRLAQEEIFGPILAVMRVASFEQALEYANGTAYALT
                     GGLYSRSPRHIARAREAFDVGNLYINRPITGALVSRQPFGGHRLSGVGAKAGGEEYLK
                     QFLVARVVSEQTLRRGFVPTL"
     gene            complement(9494..10321)
                     /locus_tag="PLS35_12895"
     CDS             complement(9494..10321)
                     /locus_tag="PLS35_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19612.1"
                     /translation="MLGTVTRKVLKTILSDTARQRILTVERVHREGGLFDADSIFYRC
                     VGTVVTWGTGSRVHSGPFAGMRYIQHALGVHSSAYCPKLLGTYEQELHPLVEALRTGH
                     PYAHVINIGAGEGYYAIGFARCLPQSRVTAYEADAESRVILGRMAEINGVAPRLTIRE
                     SCTPSGLQAAMAQPGKTLIVCDVEGAEQELLDPVAIPALTRADILVELHDFLIPGISG
                     VIRKRFENTHQITEIPALPRQLADWPLSLSLPKVYRRTALSEYRPGGMCWFWMKARG"
     gene            10729..11307
                     /locus_tag="PLS35_12900"
     CDS             10729..11307
                     /locus_tag="PLS35_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19613.1"
                     /translation="MHSTSKVICQMPPMPIETTHELIEIILAKWEGAIGEDYLGYKGH
                     VYRMFNFCLALHPCTEEENTKLAIAACFHDMGLWPDRTLDYLPASISHAAQYLSDSGR
                     EEWSEEMALMIGMHHKVRAYPDRRYPLVEVFRKGDLVDVSLGLLSCGIPRSYITDVKN
                     HFPNHGFHTFLLKTGRAWVSRHPFNPAPFMRW"
BASE COUNT         2488 a         3565 c         3333 g         2101 t
ORIGIN      
        1 agcctgttag aatgccgccg ctgctggcat ccctatccat tgcagcctac tgacaaccgg
       61 ggcgcgcgac cgacagatgc tgcggctact tgtgcttcat ggtcccaatc tcaatctcct
      121 ggggacgagg gaaccctccg tgtatgggca gatgtcatta tccgacatcg ataagtccat
      181 tacccgccgg agcggtgaac tcgggattgc ggtgcagacc aagcagtcca atagtgaggg
      241 ggagttggtc acctggatcc agaatgccag gggacatttc gacggaatca tcatcaatcc
      301 ggccgcatac acgcatacca gcattgcgat ccgtgatgcg attgccgccg tcgccctgcc
      361 gacggtggaa gtccacctct cgaacatcca tcaacgggag gagtttcgcc atcattcctt
      421 cattgccggc gttgccatcg gacaaatcgc cggtttcggc ccgaccggat acctgctggc
      481 gctggaggca ttgaccgtac atctgggtgc caaacgctca tcgtcacgca cccgagcaga
      541 cgtgagaaag aaacccgcgg cctcgcgccg ttgaacttgt tgcactaagg agagttgttg
      601 tgatttcgac cgcagagttt cggaacggca gcccgttaat ggtagaggga caaccctttt
      661 atattgtgga attccaacac gtgaaacccg gcaagggtgg agctttcgtc cggacgaagc
      721 tgaaaagcta tctctcgggc aatgtcctcg accggacctt ccgttccggc gaaaaattcg
      781 acacccccga gattgaagag tgcgatatgc agttcctcta tgccacgaac gattcctata
      841 cgtttatgga tacggagaca tacgagcagt cgacttacga aaagagtcag ctcggcagca
      901 acgccgacct gctcaaagag aacatgatcg ccaagatcct gatctatgaa cacaagccga
      961 ttacggtcgt gctgccgaac ttcatcgaac tgaaagtcgt cgatggcgaa ccgggcgtgc
     1021 gcggcgatac ggcctccggg ggcagcaaac cggtcattgt ggaaacaggc gccacaatca
     1081 aggtgccgct gtatctggaa atcggggaag tcattcgcat cgacaccaga acccgtgaat
     1141 ttgtggagcg cgtgcgttga gcccatcacg taagaagacc ggaaaaagcc ggactgccgc
     1201 taagcccatt gtgctgccga gcgcctttgc gccacgaacc tctgcggccg accagatgat
     1261 cttaaatccc gaccaggccg cgcagattca gcaactggcg gatctgctga aacgcaacca
     1321 tctgaccgaa ctggaaatcg agcgtagcgg gatgcgcatc cggattcggc atgagccggc
     1381 ggtacgcgcc acgacgacgc ataccgtgga ggccgtccac acccaatcgg cgacatccgg
     1441 cacggcaccc gcagcgcaag ctcgtccctc ggcagagaca gacggccagg tgactattac
     1501 gtcaccgatc gtcggcacct tctaccgctc gccttcgcca gacgcagacc cgtatgtgga
     1561 agaaggggac tacgtgaaga aggggcaggt attgtgcatc gttgaagcca tgaagctcat
     1621 gaacgaaatt gagtccgagg ccgacgggcg catcaccaaa attctggcgg aaagcaccaa
     1681 acccgtcgaa tatggacagc cactcttctt gatagatccc agcgccacgc cctagccgca
     1741 gaggtcatgg ggattcgtga cgagacccgt cctgaacgca tcaacctcat gacgtctctt
     1801 atctgcgacc gcctcacgcc gaccggcaag ggccgatgtg agggctgaat gagcgcgacg
     1861 gacaagggca gaatgacgca gacgcacagc aagactcgca tcggagtatc tcgtgtttaa
     1921 aaaagtattg gttgccaatc gcggagagat cgcccttcgg gtcatccggg cttgtaagga
     1981 gctcggcatc aaaaccgtgg cgattcattc cgaggctgat gccgcaagcc tgcacgtacg
     2041 ggcggcggac gaacatgtct gcgtgggtcc tcctgaggcc gccctcagct atcgcaacat
     2101 tccgaacgtc ctgagcgccg ccgaggtgac cggtgccgac gccattcatc ccggttatgg
     2161 atttctgtcc gagaacgcgc actttgccga agtctgcgag tccatcggcg taaaattcat
     2221 cggcccgacg tcagaaaaca tcgccttgat gggcgacaag tccaaagcac gcgaagtggt
     2281 cgccaagaaa ggcctcccgg tcacgcccgg gagcccgggc gagctccgga gtgagcagga
     2341 tgcgcaggag gcggccaaaa acatcggctt ccctgtgatc atcaaggcct cagccggagg
     2401 cggcggccgc ggcatgcggg tggtgaacaa gccggaagaa ttgaccagag cctttcaagc
     2461 ggcccaggcg gaggccaagt ccaccttcgg ccacgacggc gtgtacctcg aacggtactt
     2521 tctggaaccc cggcatatcg aggtgcagat cgtcgcggac aaccgcggcc atgtcgtcca
     2581 tctcggcgag cgtgattgct caatccaacg gcgccaccag aaactcgtcg aagaaacgcc
     2641 gtcaccggcc atcgacgaac ggttgcgacg cgaaatcggc cggacggcag tcgaagccgt
     2701 caaagccatc cgctactgca atgtgggcac ggtggagttt ctgctcgaca aagaccggaa
     2761 cttcttcttc atggaagtca acacacgcat ccaggttgaa cacccgatca cggagatggt
     2821 gaccgggatc gatctggtga aggaacagat ccgcatcgcc tcgggactgc cgctctcgtt
     2881 caagcagcct gacatcaagc tgaacgggca tagcttcgaa tgccggatca atgccgaaga
     2941 cccggagaag ttcaccccgt gcccgggaca gatcaccaaa tactcagctc ccggtggctt
     3001 gggcatccgc gtcgactcag ccatggaacc gaacgcggtg gtggtgccgt attacgactc
     3061 cctcatcgcg aagttgatta cccacggacg cgaccgccag gaagcgatgg cccgtatgcg
     3121 ccgggcgctg gacgagttcg tgattgaagg aatcaagacc acgatcccgc tccataggaa
     3181 gattttcaac gatccggatt ttcaaaaggg gcatgtctcc acgacgttcc tcgaccgctt
     3241 tctcgccggc caatccgcgt aaccagccta cgataccagg gagcgcgcca cgtctcagtc
     3301 cactcgacaa ctgtgcctca agggactgta tcttatcctt gatcccaccg tcaggcctga
     3361 acggccgctc acggacgtac tgcgcgtggc cgcggcatcg ggaatacgcc tgtttcaata
     3421 tcgggacaaa caggcttcga tgaaagacgc ctatcggcag gcacttgctc tccgtcaggt
     3481 ggcgacggaa gtgggggcga cactgatcat caatgaccgg tgcgatctgg cattggcggt
     3541 caaggcagat ggcgttcacc tagggcagga tgatctcccg ctagccgacg cacgtcagat
     3601 cctggggccg gaggccatca tcgggctctc aacgcataat gcgaaacagg tcagagaagc
     3661 ggtcgctctt cagccggact acatcggctt tgggccgatc ttcgggacga ccaccaagac
     3721 cgatcatgac ccggtcgtag gccttgaagg attacgcgca gcacgcacac tcaccacctt
     3781 gcccatcttc gccatcggcg ggattacgtc tcagcatgtg acggacctca cggcggcagg
     3841 ggcagacggg gtagcggtga tctcggccat tctcaaggcc caggatcttg aggcggctat
     3901 ccggagcttc cagccgccgc cttccacaca agctcggcca agtcactgat cggccgactg
     3961 ccggccacat tctctacaac ctcgggccag gcgcaatctg cgcgagtctg ataggggagc
     4021 gggccgatca agggcatcga gactcgatcc cgtaacaatg ctaccgtcga tgctgcttgc
     4081 tcctgttcaa cggagcctcg tggtgccacc gtctcattca acaccaacgc aacgaccgga
     4141 atgtttcggc gttcgagggc ttccaatgtc agcagcgcgt ggttgattcc accaagaccg
     4201 acccgtccga ctaacaggac gggaagacgg agacggctga tcaggtcagc catagcccaa
     4261 tcactgcccg tgggcacgag gaggccgccg gctccttcaa cgaccacgca tgggtaacgc
     4321 gcagccagtt gctcataccg tccaacgatg tccccgagct cgatgacccg cccttgggca
     4381 gcagcggcgg ataagggagc caccggcgca gggaaacggt aaggtgaaac gaggtcgagc
     4441 gaatccgtca cttgcgcagc cgccatcaac cgagcggcgt cggtgcgatc cgacaagcct
     4501 gcatccacgc cggtttcgac cggtttcatg acgcccaccg aacggcccat ccgcacgagc
     4561 ctgttgacca gcgccgcagc cacgagcgtt ttgccgacac ccgtgtcggt acctgtcaca
     4621 aacacgcccg tcgtccctat catgcctatt ccgtctccac gatcgattag tagagaatcc
     4681 ggctatccag attcacgatt tgtcccgaca tgcccggaag ctgcgcgagg tgacaaatgg
     4741 ttcggctcac atcctccaga ttcatcagac ggcccagcac atgatcactc agctgttccg
     4801 tggaaggaaa ggcatcaccc gccagcgcgg tgggttgcca ccccgggcag acgagattca
     4861 cgcggacatt gtgcggcccc cattcgcgtg cagctgtctg cacaagcccg acaagcccgg
     4921 ctttcgaagc cgcgtaggcg gcttggccgc tcgctccatg caatccggca taggacccga
     4981 ccacgatgat agatccgcca ccctgcttga tcatcagcgg ggccacagcc gtgaggcacc
     5041 gataggttcc cgtgagattc gtatcgatga tgcgcaacca ctcgtcttcc gggcaacgga
     5101 gcacgagatg gctggacgcg acgccggcgt tgcacaccag cacgtcgagg cggtcttgcc
     5161 gatccagcac agcatcgatc atcgcctgaa tctccagacc tgaccgtata tccgcctggt
     5221 agatggcgcc cgctcctccg tctgtggaaa gctcgcgcaa cgtttcctcg gctgcttctt
     5281 tgtggcgacc atagtgcacg ccgacccaat agccggcgcg ggcaaaggcc aggcaaagag
     5341 cgcggccgat accaccggac cctccggtaa ccaggacggt tcggagttcg cgaggagatg
     5401 acgcagatgt ctgtggttct gattgcacgg cgagcgatta tgcgagccgc gcgggaagaa
     5461 ggcaaggcag cagccagctt gccgcggaag tcctggtatg gtaccgtgat tttttgactt
     5521 ggaggacagc cggaatggtt cagaacctgt ttcgctcaac cctgattgca gtaggcattc
     5581 ttggatccgc ggccatcctc gtggggcacg atacggcctc cgccaccgaa gcagggcagg
     5641 ccatcgccca atccgcagac tattttcccg acaccatcgg cacgcgctgg caatatcgtg
     5701 gccagatttc tgaagggccg ctgcaaacga tcgagaataa gtatttcagc aacgtctcct
     5761 cggtcaccgg cacgcgtacc ctgaagggcg gcctggtcgt caccgtgttc catgacacca
     5821 atcccggtaa ccacggtccc tccgacagct tctaccggcg cgatgccgcc ggcgtggtct
     5881 actatggatc cgaaccgggc acgccgctgg agaagcagtt gacgccctac cagatcatcc
     5941 gctttccgat gaaagtggcg caatcgtttc agcagttcaa tcggaccgac atcgattttg
     6001 gcagcgatat cgacggggac ggcaccaacg aaaaagtcga cgtcgaggga ctgtcaacgg
     6061 tgttgggcca ggaatcgatc accgtgcccg ccggcaccta tcctgatgcc gtacgcgtgg
     6121 aagcgaagat gaccctgaag atccggctat cctctgtgga tcggacggcg gtcggcacgg
     6181 atgtaatgac ggcctggttc gccaaaggcg tgggactggt caagtatatc gaacgccagg
     6241 aattggccgc gctgaaggat gaccgcggaa acatcaccga catcacggaa gagctggaag
     6301 aggtcagcgt gaagtcggcg ccggggctac agggtcggca cgaatccgcg ccggagggtt
     6361 tgctcgctga cgacacgagc aaccagaaat tgtttcaggt actcttcgcc tccggccttc
     6421 gcgcctacgc ccgataaccg gtgcccgccg aatggctgtc ggctgaccaa ggcgccggta
     6481 atcgggcggt tgatgtacag attcccgaca tcgaacgcct cccgggcacg cgcgatgtgc
     6541 cggggactcc gcgaatagag gccgccggtc agcgcgtagg ccgtgccatt ggcgtattcc
     6601 aatgcctgct cgaacgaggc gacgcgcatc acggccagga tcggaccgaa aatttcttcc
     6661 tgcgccaacc ggtgcaccgg ttcgatatcc gcgatcacta ccggcccgac cgaatatccg
     6721 gggccttccg ccgaccgatc caccagcacc cgaccttcac gccgcccgat ttcgagatag
     6781 tccagaatgc gttgtttcgc gcgcgcatcg atcaccgggc ccacttgggt tgcgggatca
     6841 tctgccggac cgacacggag actcatcacc gcatcggcga ggcgttcgag aaacgcatcg
     6901 tgaatcgagt cgacgacaat cacgcgcgaa caggccgaac atttctgtcc agcatatccg
     6961 ctaaacgatt gcaccacgcc tgtcacggcc tcatcgagat ccgccgtctc atcaacgatg
     7021 accgcgtttt tcccgcccat ctcggcgatg acccgcttca cgaaccgttg tcccggttgc
     7081 tgaactcccg cggcctgcat gatgcccaac cccacgtcct tcgatcccgt aaaggcaatc
     7141 gttcttaccg ctgccgagac caccaattca cggccgagct ccggtcctcc cggaatgtat
     7201 tgcaacagcc ccttcggcac cccggcatcc aatagaatac gcgccagctg gtaccccatg
     7261 gccgaggagc gctcagacgg cttgaacagg actgggttgc ctgcaaccaa cgccgccgcc
     7321 accatgccgg tcgggatcgc caggggaaaa ttccacggcg cgatgaccac cgtgacgccg
     7381 cgcgagttcc acaacacctc gttgagctca ccgggatacc ggcccaaacg aggcggggga
     7441 tcgagccgtg ccatttcgcc agcgtaatac tccagaaaat cgatcgcctc cgcaacatcc
     7501 gcatcggcct cccgccaggg cttgccttct tcgaacacct cccaggccgc caggtcccat
     7561 cgttgatccc gcatcagtga ggccgccttc cggatcaccg cgacacgatc agcaaccggc
     7621 cgtctcgtcc aggactcctc agcagccccg gcaatctttg tcagcgccgc gacatcggcg
     7681 ggctgataac tttgcaccac cgccacgatc tgctcggggg gactgggatt gcgtgtcgat
     7741 aggtctggac cggtcggaag gtgcgccgcc agcgttgaca ggtcgagacg ttgccccaag
     7801 tgtttgcggc gccgttcgat cgcatcggcc atcgccgtac gcgccgacgc tcgcgaaaaa
     7861 tcggcaaccg gttcattcac aaattcggtc ggtgatgtat agggggaaga cttgtgctcc
     7921 accggcgatg aataggccgg gtgagacggg gcgccatcag gcggcgataa caacaacgac
     7981 agcggctgcg actcgacgta ctctttccgc aggaaggatt cgttcgacgt gttctctaac
     8041 aagcggcgga cgagataggc catgcccggc aacagttcgc cgaccggcgt atatagccgg
     8101 aggcgccgcc cgcgatcact catcgcatgc tggaacggtt ccgccattcc gaagatcatc
     8161 tgatattccc aagcgaccgg tggcaggccc agcgcctccg cgaccgcttc gatcacggcc
     8221 aggctgcgga gattgtgcgt gccgaaggcc ggcctgatca gatgcgattg ttccagcaac
     8281 agacgaacca gcgattcata attcacgtca gtctgggact tctgttcgaa cagcggcatc
     8341 ggccaaccac gctgacgata ccggatggcg tcggaatccc agtaggcacc cttcaccagg
     8401 cggatcgtga tcggcacctg ccggcgtgtg gtccatgcca gtaaccgctg cacatcgtca
     8461 taggtatcga tacggtacgc ctgcagcgcg atccccgcgt gggcgaaggt gcggtacggt
     8521 tcttcctcga agagacgcat gaagatggaa agaattaggt ccttcgtttc cgcctgttcc
     8581 atatcaaaaa tgatggaggc ggggagcgtg cccgcgacat tcagcaacgg acgcaggcgg
     8641 gccgccaccg cacgatagct gctctcggga tcgatcggat cgagttgtga gtagagggcc
     8701 gaaatcttga tcgacaactg gacacgggga agcgggccga ggtgatcgtg ctcgagcaac
     8761 ggcgaggggg accaggccgg gcagacatca ccgaggatcc gcagggctgc cagacagcga
     8821 tcccgatagg catccgcctc ccgttcgttc acactcgctt cgcccagcag atctacagaa
     8881 aacgcccgcc catcgtccca taattttcgg agcgccggca ccgcctgctc gatggacgcg
     8941 ccggcaatga agctggtcgc catctgttcc acctggtgac ggatggcctt cccgctgagc
     9001 gccgcaccca gcttcgtgga cgcgagtgcc ttgagtcccc actgcgcgcc gaagagatgc
     9061 ccgctcatgt cgccaaaata ttcttccgcc agacggacca cctgggcatc atcgcggagc
     9121 gacggcaaca catcgatgaa gcgaaacagc tgcgccttga aagcgggatc tttcatggcc
     9181 aggttgatcg ccgcctgcga ccaccaacgg gaatcaaaca ggccgggcga caatccgctg
     9241 gaacggcggg cgagatcttc accgatggta cgaatacggg gttcaagcag ggcagggtcc
     9301 agagtcatgt ggtcctccag gacgacgtga agagctcttt cctgcagtat acaccgcgcc
     9361 tctgcacttg agaatttggc tgcgcgttcg agcgagaatc atccccgagg cggacaagac
     9421 cacaaagagg aacgccatgg agaatgccgg gcaacccctg gctcaatctg catgcgcgac
     9481 ggcgagaact gcgttacccg cgggccttca tccagaacca acacatccct cccggtcgat
     9541 attccgagag ggcggttcgc cgatagacct tcgggaggct taatgagagt ggccaatcgg
     9601 ccagctgtcg aggaagggct ggaatctccg taatctggtg cgtattttca aaccgctttc
     9661 ggattacgcc tgaaatgccg ggaatgagaa agtcgtggag ttcgaccagg atatccgcac
     9721 gagtgagggc aggaatggcg accggatcaa ggagctcttg ttcggcgcct tcgacatcac
     9781 agacgatcaa ggtttttccc ggctgcgcca ttgccgcctg caacccagac ggggtgcaag
     9841 actcccggat cgtcagacgc ggagccaccc cgttgatctc cgccattcgc cccaagatga
     9901 cccgactctc agcatcagct tcataggccg tcacccggct ctgaggcagg caacgagcga
     9961 aaccgatcgc atagtaccct tcaccggcac cgatgttgat cacgtgagcg taggggtgtc
    10021 cggttcgcag agcctcgacg agcggatgga gttcctgttc ataggtcccg agcagtttgg
    10081 gacagtaggc gctgctgtga actccgagtg catgttggat gtatcgcatg cctgcaaacg
    10141 gccccgagtg cacgcgggag ccggttcccc aggtcaccac ggttccgaca cagcggtaaa
    10201 agatcgaatc ggcgtcaaaa agaccgccct cccggtggac acgctccacc gtcaagatgc
    10261 gctgccgcgc agtatccgac aggattgtct tgaggacttt acgggtgact gttccaagca
    10321 tcggaagggt ctccagcgga catagagggt gacacgtaca cgccactcta ggcgcattgc
    10381 gggcacgggt caatcggccg gaggccgcct cgcgtcgtca tttctgttca tgcgcctgta
    10441 cgtgaacagc tgacagccgc tccgtgcgca ggattataag tgattgtaca gccgcctccg
    10501 aacgtgcagc actgggaggg gtgctgtcga acaagagcgc gagccaggct ggatcggcgc
    10561 gaggagtctt gcaaattctc cgtgtcttgt tagaatccgg ctccgaccgc acgaccgcct
    10621 gcccatcgcc acaggtcggc acgcggcttg tgccagggcc gccgactcct gcgtgataga
    10681 tcctcgctca cctgccatcc tccagtcgaa cagaaaggac cacgtgccat gcactccaca
    10741 tccaaggtga tttgccagat gcctcctatg ccgatcgaga ccacgcacga gctgatcgaa
    10801 ataattcttg cgaaatggga aggggcgatc ggggaggact acctgggata caagggacat
    10861 gtgtaccgca tgttcaactt ctgcctggcg ttacatccat gcaccgagga ggagaacaca
    10921 aagttagcca tcgccgcctg ctttcacgat atgggtcttt ggcctgatcg cacgctcgat
    10981 tacctcccgg cctcaatttc ccacgccgcc caatacctgt ccgattcagg gcgggaagag
    11041 tggtctgaag aaatggccct gatgatcggc atgcatcaca aggtacgcgc ctatccagat
    11101 cgccgttacc ctcttgtcga agtgtttcga aaaggcgacc tggttgatgt ttcgttgggc
    11161 ctattgagtt gcgggattcc gcgatcgtac atcacagacg tgaagaacca ctttcccaat
    11221 cacggctttc acacattcct cctcaagaca ggcagagcct gggtgtcgag gcatcctttc
    11281 aatcccgcgc cctttatgcg ctggtagcac agactgagta ggtcaggtga ccttcacgcc
    11341 cacctggcgt gcggcagcca accgcgtttc tccccttgaa aatctgacgg cggctccgtt
    11401 acagtgcgcc gtttgccttc acccattctc taagtatggc ggtgcactgt ggcgatgagt
    11461 tgcgcacagg ttccgaagga gtacgat
//
LOCUS       DAOQEW010000120        11482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1684_length_11482_cov_6.764330, whole genome shotgun sequence.
ACCESSION   DAOQEW010000120 DAOQEW010000000
VERSION     DAOQEW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1684_length_11482_cov_6.764330"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="PLS35_12905"
     CDS             <1..1694
                     /locus_tag="PLS35_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNI19614.1"
                     /translation="YDHGVSATCDYPEWTVPDLLRRSASRFPESPALFFYGTRISFQE
                     LNELSTRFALGLRRLGVETGDRVALMLPNIPQAVIAYYGVLKAGAVVTPTNPLYVERE
                     IEGQLHDAGSDTIVALDLFYPRLQAIRERTGLPHRIILTSLRDFLPPLKRWLYPIKAR
                     WVKRWISVEKYPPVYDFCALLGRGARERDVDTEPLPTLEPGALAQIQYTGGTTGIPKG
                     VMLTHRNVVVNTMQGRFWCANFREGKEVFLGAVPLFHCYGLNTCQNLAIATGSQIILL
                     PRFHADEAVKAIQQHRVTIMSGVPMMFSMMTDSPNVRRYDLHSIRVCLCGASPLPAEV
                     QDAFERLSGVKISEGYGLTEAGPTTHCNPIERDHPPGSMGLPFPDTEARIVDLETGLT
                     DLPTGAAGELIVRGPQVMRGYWQKEADTQAVLRDGWLHTGDIVRRDEQGFFFFLDRKK
                     DVIKPWGETVYPREVEEILFQHPAVHEAVVVGMPDRHYGEAVKAFVVPAEGSSVTERE
                     LIDHCRKSLARFKVPVAVEFRSELPRTIIGKVLRRALRDEAEVPVGTAQAPRKAV"
     gene            1714..2904
                     /locus_tag="PLS35_12910"
     CDS             1714..2904
                     /locus_tag="PLS35_12910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNI19615.1"
                     /translation="MREVVIAAAARTPIGNFGGALKDLTPHKMGELAVREVMARAELD
                     PGLIDEVIIGSVGHTSDAYNVARVIALMAGLPVRTPAYSVQRNCSSGLQPFVNAYQNI
                     QSEDADVQVVGGVESMSRAPFVSRDMRWGKRLRHAEFIDSIWEGLTDAFCGQLMGLTA
                     ENLAEEFNISHEEQDHFAVESHRRAFKAIREGRLKDEVFPVMVQKTVAGREVPPVAVV
                     QDEGPNVGLTEQQLALYPPLFKEGGTVTAGNSCPLNDGAAAAIVMSGARARDLNHRPL
                     GRIKSYAFIGVEPTRMGIGPAEALPLALKRAGITLTDLNLIEVNEAFAAQYLAVERVL
                     GLKRDIVNVNGGAIALGHPVGMTGTRLVMTLLYEMKRRGAALGAVAMCVGGGQGAAMV
                     LEQI"
     gene            2979..4226
                     /locus_tag="PLS35_12915"
     CDS             2979..4226
                     /locus_tag="PLS35_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNI19616.1"
                     /translation="MYIYKVGVVGAGTMGAQIAEVVSFAGLPVVLADREEALARRGVE
                     SVRAIYQARVAKGKMTPEQLEEKMLLVTASPNLERLHDADLVIEAVSEDLLLKQRVFQ
                     ELDRICSRSAILASNTSALSISAIGSATKRPDKVIGLHFFNPAYAMPLVEVIPGLGTD
                     PQTVDDVVGFSESLRKQPVIVKECAGFLVNRLLSPYLNEAVWCLQDGDVSIKEIDQDM
                     VSFGMPVGPFTLLDTVGLDIALDVARILHRSYGPRMAPAPLLEAFVKAGRMGLKTGKG
                     LYDYGAAEEGGADQHLERLRAQARQLTGRSSMKWSPLRPLVAMVNEAVIALQEGVASA
                     RDIDFAMAAGTGFPSDKIGPLHLADQLGIDRVLQELEELQDRVGPRFWPAPMLRRLVD
                     AGFTGQGAGRGFFSYEPTIARMR"
     gene            4234..5022
                     /locus_tag="PLS35_12920"
     CDS             4234..5022
                     /locus_tag="PLS35_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNI19617.1"
                     /translation="MTTTAGSMLACHRDGAVATVTIHHPPANTLTPELLGELSQAFEM
                     FATDEAIKVVVLTGTGRFFIAGADIRLLAAIASSQEGASIARQGQAILNRIESLEKPV
                     IAAINGVCLGGGMELALCCHIRLVAEGSRLGQPEINLGIMPGFGGTQRLPRIVGQSKA
                     LELILTGDPISAQEAKALGLVSAVFPPEDLLHQAHSLARKMASKSLPALRASLRAIRS
                     GADMSLPEGLALEAVLFGGLCETEDKREGLAAFLEKRQPRFIDR"
     gene            5059..6279
                     /locus_tag="PLS35_12925"
     CDS             5059..6279
                     /locus_tag="PLS35_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNI19618.1"
                     /translation="MASAFGKRALALGGGGFTGYLFEIGALTALDDLFEDGVSMNDLD
                     LYVGVSAGAAAVSLIANGVKPRDILETNLSGTRPYYFDHRNVFSPAIGEGLKTFWRVT
                     RQLVPLVKYYVRHYGEMTLIDLLDKAQDALPSGIYTLEPFALYLAGLYKAKGLSNSFT
                     GLSKELYIPAIDLETGESVLFGEEGWREVPISRAVTASSAVPIYFCPVRIQGRDYIDA
                     GIGRMAFFEVAVHKKVDFMIMINPMARPLQRRLPVDVPDHGSSVGRLRDRGFLSIGEQ
                     ASRINFDARFSQALERFQHEYPAKEVLVISPVEEDALLFERSFLSYRDRIHLLRAGYG
                     SVMTLVRDQFDHVSAQLASYGMTISRARFEDRAVQRLARLNHVAWTSAPRVVPNAATA
                     ARIGGIGAAWRKAR"
     gene            6302..8224
                     /locus_tag="PLS35_12930"
     CDS             6302..8224
                     /locus_tag="PLS35_12930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNI19619.1"
                     /translation="MANKDRMFTGSLAVAEAARDTRLYSGFLSGLFEGNVRRHLYSRA
                     SLPARSGAASEFLDRLRHLLIEKVDPETIDREGLIGEDVFAALREIGAFGIKIPRRYG
                     GLDFTQSEYHEAATLLGSHDAATTVLLSAHNSIGAAEPVKLVGNSEQQQRLLPRLARG
                     EVSGFALTEKGAGCDIWDLKTYAIPVHEQGLVVGYRLTGDKLYTTNAPRGHHAFLASW
                     LVVIAQIVDSPQDIHRAKEERRFGAFVVDTHTRGCSCTRLSFMGVRGIYNGQVHLREV
                     WVPVADRLGAEGEGLRRALESLTVGRLTLPAACLGNLKQCLWLARLRAQQRKQYDRPI
                     GEHTDIGAKIVLMASRVLALEAIVKITGIWADTKQDVRLESAAAKILATEWLLESLLD
                     LFRIYGGRAFETPDSLRLHGDLPAPIERMIRDALINVIWEGSNGILTLWIGREGLSQY
                     FTHGQAFLDCHIADMVRATPFFVNTAARSFGGLTVYEQDSEAGSFDPLWERFVARKSR
                     ELARTTLWVAARDRQGLARKQRLMTRLVSAAMHLFAVETALWYKSRQEQRGKPHLDQL
                     VSYFCSRTSAAFNPEPLLSVRTSSWDDDSNLYPLAKAILSGHVEWLEEGIIPCRAIEK
                     DVASSEERMVGASARG"
     gene            8309..9292
                     /locus_tag="PLS35_12935"
     CDS             8309..9292
                     /locus_tag="PLS35_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI19620.1"
                     /translation="MMEIARYFDAVKDRYELSSDYALAEKLGIAQPEANLMRRGLKIP
                     KPELCIKMAKLLDKNPVELLLIAQKDKAPKQAKEYWTLALTAVDVMLHVPKRPRYLPK
                     KVEAIGRELKQLEAQTLTYEGAAANAEAVRLMETAEQSVDAMMERWNIWKKGEALYPN
                     YLLANQAAARRHVRIRRLLILTQEQMQHDATVADAIQVMDDQRRAGVKVFYAFREALV
                     HSPAFQRLEEDFRKYGAAEDMNTAMFDREILIFSQTYGSVPLGMVGTPTPITMINRLQ
                     ISWKPEMIRELDPAPLFDMTRYVFDYQGSGPFHEQLAYFRRSIRELPIRAV"
     gene            9267..10694
                     /locus_tag="PLS35_12940"
     CDS             9267..10694
                     /locus_tag="PLS35_12940"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNI19621.1"
                     /translation="MNFPFEPFRIKVVEPVKRTTREERDRLLREAGYNLFHVPAESVA
                     IDLLTDSGTSAMSDSQWAGMMLGDESYAGSKNYHHFETVVRGLFGYRYVIPTHQGRAA
                     EHVLFSTVLKPGMCVPNNIHFDTTRANVEHQEAEAKDLVIKEAYDPHCELPFKGNMDV
                     ARLEETIKRVGPSQIPFVMITITNNSAGGQPVSMANIRATRQVLNRYGIPLFFDACRF
                     AENCFFIREREPGYADRPIRDIARELFRLGDGCLMSAKKDGLVNIGGFLALNDERWAQ
                     AVTNMLILVEGFPTYGGLAGRDLEAMARGLEEVLDEEYLRYRIGQVQALGALLEEGGV
                     PIVKPTGGHAVYVNAKEFLPHLQQSQFPAQALAVALYRDYGVRTVEIGTVMFGKRDPA
                     TEETIYPALELVRLAIPRRVYTSQHLSFVAEALVDLYQRRHSIGGLVMTYEAPRLRHF
                     TARFEEETLAEMLWPGAGFAAAPLS"
     gene            11117..>11482
                     /locus_tag="PLS35_12945"
     CDS             11117..>11482
                     /locus_tag="PLS35_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19622.1"
                     /translation="MACVKKSVGQASGIYGAADARLDASGRSSNRGDVLRRWRGTVAG
                     VAAVLLLGALVVPAHSQTGSQLGPLPPKPPQLPVVPPPAQPPALDLPTPTPPGALDQA
                     AKGPKIMVKDIRLVGNTAFT"
BASE COUNT         2278 a         3138 c         3660 g         2406 t
ORIGIN      
        1 gatatgatca tggtgtctcc gctacctgtg actatccgga gtggacagtt ccggatttgc
       61 ttcggcgctc cgcctcccgg ttcccagaat cgcccgccct cttcttctac ggcacacgta
      121 tttccttcca ggagttgaac gaactaagca cccggttcgc gctgggactg cgacggttgg
      181 gcgtcgagac cggcgatcgg gtcgccctca tgttacccaa tatcccgcaa gcggtgatcg
      241 cgtattacgg cgtgttgaag gcaggagccg ttgtgacgcc gacgaatcct ctgtacgtgg
      301 agcgtgaaat tgagggccag ctgcatgatg ccggcagcga taccattgtc gcattggatc
      361 tgttttatcc tcgcctccag gctatacgtg aacgcactgg attgccccac cggatcattc
      421 tcaccagtct gcgggatttt ctcccgcccc tgaaacgatg gctctacccg atcaaggcgc
      481 gttgggtgaa acgatggatc tcggtcgaga agtatccacc ggtgtatgac ttctgtgcct
      541 tgctcggccg gggagccaga gagcgtgatg ttgataccga gcctcttccc accttggaac
      601 cgggcgcctt ggcgcagatt cagtataccg gcggaacaac cggtataccg aaaggcgtga
      661 tgctcacgca tcgcaatgtg gtggtcaata ccatgcaagg ccgtttctgg tgtgcgaact
      721 tccgcgaggg aaaagaagtg tttctcggcg ctgtgccgtt gttccattgc tacgggttga
      781 acacgtgcca gaatctggca attgcaaccg gctcgcagat tattctcctc ccgcggtttc
      841 atgccgacga ggcagtgaag gcgattcagc aacatcgcgt gaccatcatg tcgggcgttc
      901 cgatgatgtt ttccatgatg accgattctc cgaacgtccg ccggtacgac ctgcattcga
      961 tacgggtgtg cctctgcgga gcgagtccgc ttccggcgga agtgcaggat gcctttgaac
     1021 gtttgagcgg agtgaaaatt tcggaaggct acgggttgac ggaggcgggc cccacgacgc
     1081 attgcaaccc gatcgaacgg gaccatcctc ccggctcaat ggggctgccg tttccggaca
     1141 cggaggcgag gatcgtagat ctggaaaccg ggctcacaga ccttccgacc ggagcggccg
     1201 gtgaactgat tgtccgcggc ccccaagtaa tgcggggata ttggcagaaa gaggccgaca
     1261 cgcaggcggt attgcgagac ggctggcttc atacgggaga cattgtcagg cgggacgaac
     1321 aggggttttt ctttttcctc gatcgcaaga aggacgtcat caagccatgg ggcgagaccg
     1381 tgtatccccg ggaggtcgaa gaaattctct ttcaacaccc ggcggtgcac gaagcggtgg
     1441 tggtgggcat gcctgatcgt cattacggag aagcagtgaa ggcctttgtc gtgcccgcag
     1501 aaggatcgtc tgtgacggaa cgggagctca tcgaccactg ccgcaagtct ctggcgcgat
     1561 tcaaagtgcc ggtggccgtt gagttccgtt ccgagttgcc gcgaaccatt attggaaagg
     1621 tgctgcgaag ggctttgcgt gatgaggctg aggttcctgt cgggacggca caggcgccgc
     1681 gtaaggccgt gtgagagtgg aagggaggct tccgtgagag aggtcgtcat tgccgccgcc
     1741 gcgcgaaccc ccatcggcaa cttcggtgga gcattgaaag atctcacgcc gcataaaatg
     1801 ggcgaactag cggtgcgtga agtgatggca cgtgctgaat tggatcccgg gctcatcgac
     1861 gaagtgatca tcggctcggt gggacacacc agcgatgcgt acaatgtcgc gcgtgtgatc
     1921 gcgctcatgg ccggtcttcc ggtgcggact cctgcctatt cggtgcagcg gaattgttcg
     1981 tcgggcctcc agccgttcgt gaacgcgtat caaaacattc aaagcgaaga tgcggatgtg
     2041 caagtggtcg ggggagtcga gagcatgagc cgtgccccgt tcgtttcacg cgacatgcgg
     2101 tggggaaagc ggcttcgtca cgcggaattt atcgacagca tctgggaagg gttgacggat
     2161 gccttttgcg gtcagttgat gggcctgacg gcggagaact tggcggagga attcaatatc
     2221 agccatgagg agcaggatca ctttgccgtg gaaagtcacc gccgggcgtt caaagccatc
     2281 agagaaggac gtctcaaaga tgaggtcttc ccggtcatgg ttcaaaaaac ggtggccgga
     2341 cgcgaggtcc cgccggtcgc agtggttcag gatgaaggcc cgaatgtcgg cttgaccgaa
     2401 cagcaactcg cgctctaccc gccgttattt aaagaaggcg ggaccgtgac ggcgggaaac
     2461 agttgtccgc tcaatgatgg tgccgcggcc gcgattgtca tgtcgggcgc ccgggcgcgc
     2521 gatctgaacc accggccgct gggccgcatc aagagttacg cctttatcgg ggtggagccg
     2581 acccgcatgg gcatcgggcc ggcagaagca ctcccactgg cgctcaagcg cgccgggatc
     2641 accctgactg atctgaacct gatcgaagtg aatgaagcct ttgcggcgca gtacctcgca
     2701 gtggagcggg tattgggact caaacgggac atcgtcaatg tgaacggcgg cgcgattgcc
     2761 ttggggcacc cggtcggcat gaccggcacg cgcctggtga tgacacttct ctatgaaatg
     2821 aagcgtcgcg gtgcggcgct gggcgcggtg gccatgtgtg tcggcggtgg acagggcgcc
     2881 gccatggtgc tggagcagat ctgaggaaga gtgttgagtg atgagtgagt gagctgcagt
     2941 gaccaagatg gacctcaccg ggaacgtgtg gtgacgccat gtatatctac aaagtcggag
     3001 tggttggtgc aggcaccatg ggggcacaga ttgcggaggt ggtaagtttt gccggcctgc
     3061 cggtggtatt ggcggaccga gaggaagctc ttgccaggcg aggcgtcgaa tcggtacggg
     3121 cgatttatca agctcgtgtc gcgaagggca agatgacgcc cgagcaattg gaagaaaaga
     3181 tgctgctggt gacggcatcc ccgaatctgg agaggctcca tgatgccgat cttgtcatcg
     3241 aagccgtgtc tgaagacctg ctgctcaaac agcgagtgtt ccaggagctg gaccggatct
     3301 gttcccgcag cgccattctt gccagcaaca cttccgcgct ttcgatctcg gccattggat
     3361 ccgccaccaa gagaccggac aaggtcatcg gtctgcattt tttcaatccg gcctatgcga
     3421 tgcccctggt ggaagtgatc cccggtctcg gcaccgatcc gcagacggtc gatgatgtcg
     3481 tcggtttttc ggagagcctg cggaagcaac ccgtgatcgt gaaggagtgt gcgggcttcc
     3541 tcgtcaatcg gctgttgtct ccctatttga atgaagccgt ctggtgcctg caggacggcg
     3601 atgtctccat caaagagatc gaccaggaca tggtttcctt cggcatgcct gtcggaccat
     3661 tcactctgtt ggataccgtc ggcttggata ttgcgctcga tgtcgcgcgc attctgcacc
     3721 gttcgtatgg gcccaggatg gcgcccgcac cactgctgga ggcgttcgtc aaggcggggc
     3781 ggatgggcct caagacgggg aaggggttgt atgattacgg cgccgccgag gaaggtggcg
     3841 ccgatcaaca cctcgaacgt ctgagagcgc aggcacggca gcttacggga cgaagttcaa
     3901 tgaaatggtc ccccttacgt cctctcgttg ccatggtgaa cgaggccgtc attgcgctcc
     3961 aggaaggtgt cgcgtccgcg cgtgacattg actttgccat ggcggcggga acgggatttc
     4021 cgagcgacaa gatcggcccg ctccatctgg ctgaccagct tggtatcgat cgcgtgcttc
     4081 aggaattgga ggagcttcag gacagggtgg ggccacggtt ctggccggcg ccgatgttgc
     4141 gtcgtttggt ggatgccggg tttacagggc agggggcggg gagagggttt ttctcttacg
     4201 aaccaaccat tgctcgaatg aggtgaggtt cccatgacta ccacggccgg ttcgatgctc
     4261 gcatgtcacc gtgatggcgc agtggcgacc gtgacgattc atcatcctcc ggcgaatacg
     4321 ctgacgccgg aattgctcgg cgagctcagc caggccttcg agatgttcgc caccgatgag
     4381 gccatcaaag tcgtcgtgct gacgggcacg ggccggtttt tcattgccgg ggcggacatc
     4441 cgtctgttgg ccgcgattgc ctcctcgcag gaaggcgcat ccatcgcgcg gcaagggcag
     4501 gctatcctga atcggatcga gtctctggaa aaaccggtca tcgcggcgat caatggagtc
     4561 tgtctcgggg gaggaatgga attggccctc tgttgtcaca tcagactcgt ggctgagggc
     4621 agccggttgg gccagccgga aatcaatttg ggcatcatgc cgggatttgg cgggacgcag
     4681 cggcttcccc ggatcgtcgg tcaatccaaa gcgctggaac tgattttaac gggcgatccg
     4741 atctcggcgc aggaggcgaa ggcgcttgga ttagtctctg cggtcttccc gccggaggat
     4801 ctgttgcacc aggcccacag tctggctcgc aagatggcct ccaaaagtct gccggccttg
     4861 cgtgcgtcgt tgcgggccat ccgtagtggt gcggacatga gtctgccgga aggcctcgcc
     4921 ttggaggcgg ttctgttcgg cggtctctgc gagacggagg ataagcggga aggcctggct
     4981 gcgtttttgg agaaacgtca gccgcggttc atcgaccgtt aacgactgtg caggcaaccc
     5041 tgccgatgaa agggtgtcat ggcatcggcg ttcggcaagc gggccttggc cttgggcgga
     5101 ggaggcttca ccggctacct ctttgagatc ggggccttga ccgcgttgga cgatttgttc
     5161 gaggacggcg tgagtatgaa cgacctcgat ctctacgtgg gtgtcagcgc aggtgcggcg
     5221 gctgtatcgt tgatcgccaa tggcgtgaag ccccgagaca tcctcgaaac caatctgtcc
     5281 gggacgaggc cctattattt cgatcatcgc aacgtgttct ctcctgccat tggggaaggg
     5341 ctcaagacgt tctggcgcgt gacccgccaa ctcgtgccgc ttgtgaaata ttatgtgcgg
     5401 cactacggtg agatgacgct catcgacctt ctcgacaagg cccaggatgc gctcccgagc
     5461 gggatctata cgctcgaacc attcgccctc tacctggcgg gcctctacaa ggccaaaggg
     5521 ttgagcaatt cctttacagg gctgtcgaag gaactctata tccccgccat tgatctggaa
     5581 acaggggaga gtgtcctgtt cggcgaggaa ggttggcggg aggtgccgat ttctcgcgcg
     5641 gtcacggcct cttcggcggt tcccatctac ttctgccccg tacggattca agggcgcgat
     5701 tacatcgatg caggaatcgg gcgcatggcg tttttcgaag tggccgtcca taagaaggtg
     5761 gatttcatga tcatgatcaa tccgatggca cgcccgcttc aacgccggct gccggtcgat
     5821 gtcccggacc atggcagcag cgttggtcgt ttgcgggatc gcggattttt atcgatcggt
     5881 gaacaggctt cacgcatcaa cttcgacgcg cgcttctctc aggctttgga acggttccag
     5941 cacgagtatc ccgcgaagga agtcctcgtc atttcgccgg tggaggagga cgcgctcttg
     6001 ttcgaacgga gttttttgag ttaccgcgac cgtatccatc tcctgcgcgc cggatatggc
     6061 tcggtcatga ccttggtcag agatcaattc gatcacgtat ccgcacagct tgcttcatat
     6121 ggcatgacca tctctcgcgc ccgcttcgag gaccgtgctg tacaacgtct cgcgcgactg
     6181 aatcacgtcg catggacttc ggccccgcgc gttgtgccga atgcagccac cgctgcgcgt
     6241 atcggtggca tcggcgccgc ctggcgtaaa gcccgctgac tcagggcggc agagggcatc
     6301 aatggccaac aaagaccgga tgttcacagg atcgctcgcg gtagccgaag ccgctcgtga
     6361 cacgcgactc tattcagggt ttctctctgg tctgttcgag gggaacgtcc ggcgtcatct
     6421 gtattcgcgc gcgtcgctgc ctgccaggag cggcgcggca agcgagtttc tcgacaggct
     6481 tcgccatctg ttgattgaga aagtggaccc cgaaaccatc gatcgagagg gcctgatcgg
     6541 agaggatgtc ttcgcggcgt tgcgggagat cggggcgttt ggcataaaaa ttccacgcag
     6601 atacggcggg ctcgacttca ctcagtcgga atatcacgag gcggcgacgt tgctcggcag
     6661 tcatgatgcg gcgaccaccg tgttgctgtc ggcgcacaat tcgatcgggg cggccgaacc
     6721 ggtcaagttg gtcgggaact ccgagcagca acaacgattg ctgccgcgat tggccagggg
     6781 agaggtctcc ggatttgcgc tgacggaaaa gggggcgggt tgtgacatct gggatctcaa
     6841 aacctatgcg attcctgtcc atgagcaggg cctggtggtt ggatatcgac tgaccggcga
     6901 taaactgtat acgaccaatg cgccgcgggg gcaccatgca tttctcgcgt catggctcgt
     6961 cgtgatcgca cagattgtcg actcacccca ggacatccat cgcgccaagg aagaacggcg
     7021 tttcggggcc tttgtcgtcg acacccacac gcgagggtgc tcctgcactc gccttagctt
     7081 tatgggcgtt cgcggtatct acaatggtca ggtgcacttg cgcgaggtgt gggtgccggt
     7141 ggcggatcgg ctgggagcag agggcgaggg gctgcggcgc gccctggaaa gcctgaccgt
     7201 aggacgactc acgctgcctg ccgcctgcct cgggaatctc aagcagtgtc tctggttggc
     7261 tcgtctacgc gcgcagcagc gtaagcaata cgatcgcccg atcggcgaac acaccgacat
     7321 cggcgcgaag atcgtgctca tggcttcccg cgtgctcgca ctggaagcca ttgtgaaaat
     7381 caccggcatc tgggccgata cgaagcagga tgtacggttg gagtccgcgg ccgcaaaaat
     7441 cctcgcgacg gaatggctcc tggaatcgtt gctcgacctg ttccgcatct atggcggacg
     7501 tgcgtttgag accccggact ccctgcgtct ccatggcgac ctgccggcgc cgattgaacg
     7561 gatgatccgt gacgcgctga tcaacgtcat ttgggaaggc agcaacggaa tcttgaccct
     7621 ctggatcgga agagaagggc tgtcacagta ttttacgcat ggccaggcgt tcctcgattg
     7681 tcacatcgcc gacatggtgc gggccacgcc gtttttcgtc aacaccgcag cgaggtcatt
     7741 cggcggtctg acggtatacg aacaggactc agaagccggc tcgttcgatc cattgtggga
     7801 gcgtttcgtg gcgcgaaaga gtcgcgaact ggcccgcacg acgttatggg ttgccgcgag
     7861 agaccggcag ggactggccc ggaagcaacg gttgatgacg cgcctggtca gtgccgcgat
     7921 gcatctcttt gccgtcgaaa cagcgctgtg gtacaagtcc cggcaggagc aacgcggcaa
     7981 accgcatctg gatcaactgg tcagctactt ctgttcgaga accagcgccg cgtttaatcc
     8041 tgagccgttg ctttcagtgc ggacgtcgtc ttgggatgat gattccaatc tgtatcctct
     8101 cgcgaaggcc attctctcgg ggcacgtcga gtggttggaa gagggcatca ttccttgtcg
     8161 cgcgattgaa aaggatgtcg cgtcgagcga ggagcggatg gtcggcgcgt ctgcaagagg
     8221 ttaaagactt ggcggttggg tgtggtgaat atcagtgcgt gtctgtagat cgccgggtca
     8281 gtgaagatca cgagttgccg ggagcgacat gatggaaatt gccaggtact ttgatgcggt
     8341 caaggaccgg tatgagttga gcagtgatta cgcactggct gaaaagctgg ggatcgcgca
     8401 gcctgaggcg aatttgatgc ggcgcggcct gaagatcccc aagccggaac tgtgcatcaa
     8461 gatggccaag ctgctcgata aaaatccggt cgaactgctg ctgatcgcgc aaaaagacaa
     8521 ggctcccaaa caggccaaag aatactggac cctggccctc acggcggtag atgtcatgct
     8581 gcatgtgccg aagcggcccc ggtatctgcc gaagaaggtg gaagccatcg gtcgggaatt
     8641 gaaacagttg gaggcgcaaa ctctgacgta tgaaggggcg gcggcgaacg ccgaggcggt
     8701 gcgtctgatg gagacggcgg aacagtctgt cgatgccatg atggaacgct ggaatatttg
     8761 gaagaaaggc gaagcgctct atcccaacta tctgctggcg aatcaggcgg cggcgcggcg
     8821 tcacgtccgg attcggcggc tgctgattct cacgcaggag cagatgcagc acgacgccac
     8881 cgtggccgac gcgatacagg tgatggatga ccagcggcgt gcgggcgtga aagtgtttta
     8941 tgccttccgg gaggcgctgg ttcactcgcc ggctttccag cgactggaag aagacttcag
     9001 aaaatacggc gcggccgaag acatgaatac ggccatgttc gacagggaaa ttttgatttt
     9061 ttcgcagacc tacgggagcg tgccgctcgg gatggtcggc acgccgacgc cgattacgat
     9121 gatcaatcgg ctgcagattt cgtggaagcc ggagatgatt cgagaactcg atccggcgcc
     9181 gctcttcgac atgacgcgat acgtattcga ctaccaggga tccggaccgt ttcacgagca
     9241 gttggcctac ttcaggaggt cgatccgtga acttcccatt cgagccgttt aggatcaaag
     9301 tcgtcgagcc cgtcaagcgc accacccgcg aggagcgtga ccggttgctt cgcgaagccg
     9361 ggtacaacct cttccatgtc cctgccgaaa gtgtcgccat cgatctgttg acggatagcg
     9421 gaacatcggc catgagtgat tcccaatggg ccgggatgat gctcggcgat gagtcgtatg
     9481 cgggcagcaa gaattaccat cacttcgaaa cggtcgtgcg cggattgttc gggtaccgct
     9541 acgtcattcc cacgcatcag gggcgggcgg ccgaacatgt gttgttttca acggttctca
     9601 aaccaggcat gtgcgtgccg aacaacattc attttgatac gacccgggcc aacgtcgagc
     9661 accaggaggc ggaggcgaag gatctggtca tcaaagaggc atacgatccg cattgcgaac
     9721 tgccgtttaa gggcaatatg gacgtggctc gactcgagga gaccatcaag cgggtcgggc
     9781 cttcgcaaat cccctttgtc atgatcacca ttaccaataa cagcgccggc ggccagccgg
     9841 tctcaatggc caatatccgc gcgacgaggc aggtgctgaa tcgttacggc atcccgttgt
     9901 tcttcgatgc ctgccggttt gcagagaatt gtttttttat ccgggagcgc gaaccggggt
     9961 atgcggatag accaattcgt gacatagcca gagaactgtt ccgcctaggc gacggctgtc
    10021 tcatgtcagc caaaaaggat ggcctggtca acatcggtgg attcctggcc ctcaatgatg
    10081 agcgatgggc gcaggcggtg accaacatgt tgatcctcgt cgagggattt cctacctacg
    10141 gcggactggc agggcgagat ctggaagcga tggcgagggg gctcgaggaa gtgctcgacg
    10201 aggagtatct ccgctatcgc atcggacagg tgcaggcgct gggagccctg ctggaagagg
    10261 gcggcgtccc gatcgtgaag ccaaccgggg gccatgctgt ctatgtgaat gcaaaggaat
    10321 tcctgccgca cctgcaacaa tcccaattcc cggcgcaggc ccttgctgtg gcactgtacc
    10381 gggactatgg cgttcgcacc gtcgaaatcg gaacggtcat gtttggcaag cgggatccgg
    10441 cgaccgagga aacgatctac ccggcattgg aactcgtgcg attggcgatc ccccgacgag
    10501 tctataccag ccagcatctg tccttcgtgg cggaagcgtt agtggatctc taccaacgcc
    10561 ggcattccat cggagggttg gtcatgactt acgaggctcc tcgattgcga cattttaccg
    10621 cccggttcga ggaagagact cttgccgaga tgctatggcc cggcgcggga ttcgccgctg
    10681 cgcctttgtc atagcgggtg gggctgcatc ggcatatacg tacatcatcg ggccggatag
    10741 accatgtcga tacaacgacc gactccgcgt tgacctgcct tcacgtgtcc catctcagaa
    10801 accgtgactc tcctgtcgcc tgcgctgttc caacctgacc cattcatttc ccccgaccaa
    10861 aaaactttgt ggccccagga gtaatttgcg tcgagacatg ttataagcag ttccttcgca
    10921 ggacggattc tccaaacacc tacgtatcgc cacggccgat agccgactgt cgcgcgactg
    10981 cgagttgttt ctgaagagtg cgtgttgcag atcgcagcag cggtgtgatt caggacaaat
    11041 atcgaaagcc gatgatttta cgggcactga ttcacgaatc tgcgtgcccc gtcatcaaga
    11101 aagaggggag gccttgatgg cttgtgtgaa gaaatcagtg gggcaggcat caggaatata
    11161 cggggcggct gacgcgcgtt tggatgcgtc cggtcgttca tccaatcgcg gggatgtgtt
    11221 gcgtagatgg cgtggaaccg ttgctggtgt ggcggctgtg ctgttacttg gcgcgctggt
    11281 cgtgcccgct cactcacaaa ccggctcgca gctcgggccg ttgcctccga aaccgcccca
    11341 gttacccgtt gtgcctccgc ccgcacagcc ccctgcgctt gatctcccga cccccacgcc
    11401 tcctggtgcg ctcgatcagg cggccaaggg acccaagatc atggtcaagg atatccggct
    11461 ggtcggcaat accgccttta cg
//
LOCUS       DAOQEW010000121        11465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1691_length_11465_cov_9.568449, whole genome shotgun sequence.
ACCESSION   DAOQEW010000121 DAOQEW010000000
VERSION     DAOQEW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_11465_cov_9.568449"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /gene="nuoL"
                     /locus_tag="PLS35_12950"
     CDS             complement(<1..1826)
                     /gene="nuoL"
                     /locus_tag="PLS35_12950"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNI19623.1"
                     /translation="MMYALIPLLPLFSFLIVGIGEQWIKDRAHLVAVPAMVGSFLLSL
                     MALHDVATGQAITVNLYTWLTSGNLDIHIGISIDRLTAVMLILVTTVSTLVHIYTIGY
                     MHGEPGYARFFAYIALFTFSMLMLVMADNLLQLFVFWEAVGLCSYLLIGHWYERASAC
                     AAATKAFLVNRVGDFGFILGLFLVWYSFGSLDYATVFAQAGQLASKTTNLLGPFGGTW
                     DVSVMTMICLLLFTGAVGKSAQVPLHVWLPDAMEGPTPISALIHAATMVTAGVFMVAR
                     LAPLYNLSPTAMTVVALVGALTMMLGATIALTQTDIKRVVAYSTMSQLGYMVMACGLG
                     AYSAGMYHLLTHGAFKALLFLGCGSVIIALHHEQDMRHMGGLKDKLPVTYWTFLVGSL
                     ALAGFPLTAGFFSKDDLLVSSWSAGPLGQVLTICGLVTAGLTAFYSFRLVFVTFWGKS
                     HVDPHHAGHVHEPSTTMTAPLMVLAVLSIVAGYLGIPAFLEPVFHGEGAGAHHEGSAA
                     LGIMALATLMGLTGIGAAYYLYVLNPGLPDRLAQQWRAAYELSLHKWYIDEAYDRSFV
                     RPTLSAAQGLWKHVDVAIIDGAVNGVARAVAWGGWLIRLTQSG"
     gene            complement(1843..2148)
                     /gene="nuoK"
                     /locus_tag="PLS35_12955"
     CDS             complement(1843..2148)
                     /gene="nuoK"
                     /locus_tag="PLS35_12955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNI19624.1"
                     /translation="MTVPLSYYLVLSGFVFLTGVVGVLIRRNIIVILLSVELMLNATN
                     INFVAFSEYFHNVAGQVFVFFALTVAAAEVAVGLAIIIALHRTNSSIYIDDLNLLKR"
     gene            complement(2160..2675)
                     /locus_tag="PLS35_12960"
     CDS             complement(2160..2675)
                     /locus_tag="PLS35_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNI19625.1"
                     /translation="MDHIFFFYFAAVIATTSVLVVALRNPVYSALALLIMFFHVAGLY
                     VTLHAEFLAAVQIVVYAGAILVLYLFVVMLLSIKSEERYHNQLPVAGLLGVMLCTEII
                     LLLIQSRTAASTQAVPADPVTGSGNTEMVGEALYSTYLFPFEVASLILLVAMIGAIIL
                     AKKDINEQVQQ"
     gene            complement(2803..3378)
                     /gene="nuoI"
                     /locus_tag="PLS35_12965"
     CDS             complement(2803..3378)
                     /gene="nuoI"
                     /locus_tag="PLS35_12965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNI19626.1"
                     /translation="MNVAITAQPKRKPSFSDWLKTLTFYELLVGMKATLKHLLNYKPI
                     TLQYPHEKRLLPENYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSGEVPGEPT
                     KRYSKEYYMDMTRCLFCGLCVDACPVDALGMTREFEWAVYDKRQLHLNKQQLLAIGDR
                     AYPVREKRLELQHPNVAFFNVTFKHLPQKEN"
     gene            complement(3388..4455)
                     /gene="nuoH"
                     /locus_tag="PLS35_12970"
     CDS             complement(3388..4455)
                     /gene="nuoH"
                     /locus_tag="PLS35_12970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNI19627.1"
                     /translation="MEIGLRLAVSLAQIAAVMGVVMLTVMILTLAERKVLGWMQDRMG
                     PMEVGPYGVLQPIADGLKLFFKEDIIPAGANKFLFTLAPILALVPAMIGFAVIPFGPT
                     MTIELFGMQVKPFVISDINIGILYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAAQ
                     VISYELNVGLAIVGVILLSGSLSLVKITEAQAGGFWNWFIIAMPFPQIFAFVVYVISS
                     VAETNRVPFDLPEAESELVAGFFTEYSGMRFAFFFIAEYANMILVSCVAAALFLGGWN
                     APYPGTILGHLGLDALAWIENVVWFAAKVYFFLFLFFWLRATLPRLRYDQLMRFGWKV
                     MLPIALGNIVLTAIAAYFFPR"
     gene            complement(4480..7149)
                     /gene="nuoG"
                     /locus_tag="PLS35_12975"
     CDS             complement(4480..7149)
                     /gene="nuoG"
                     /locus_tag="PLS35_12975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNI19628.1"
                     /translation="MPDQKPETVRLTIDGTTVAVPKGTLVIEAARRVGVMIPHFCYHP
                     KLKPDANCRMCLVEVEKMPKLQTACSTPVAEGMAVRTATTTVDDAHKSVLEFILANHP
                     LDCPVCDQGGKCDLQDFSHQYTPTTSRFTETKRIFQKEYFSPLIETQMNRCVQCLRCV
                     RYCDEIMDVKALAPVGRGTMTEIKHFGPHELDCEFCGGCIQICPVGAITSRLSMYEYR
                     PWMLKRADTICTFCGDGCRITVQTKGNELIEVNSSHGAGRNNGDLCARGFFGFHASTH
                     ADRLTHPLIRRDGVLVQATWEDALEYVAEQALRVKLANGPDAFGGLISSRCTNEDLYV
                     FQKFMRQVIGTNRIDSSARYGHLSGVRALQRVQGTHRWTIAFEDIVAANALLLVGTNI
                     TETSPITGLKVKEAVKKRQASLVTIESLEPAVDTLSNIANLATQHFSAHPAQIGNNVL
                     GLIKAVIDGNLVDAGLAEQSPAFVQRLSAATQQISWDTLETATGHTRAQWAETAKLLA
                     AAKRVVILVGNGVLRHPGGEGTTTNLLDLLILLGKLNQPGCGLGPLAEENNDQGAVEM
                     GAVPEFLPGPAPLNDQAARDRLASLWREELPRTAGISLTEMLAAANSGSLKAMFVVGE
                     NPIGTLPTGAKAKEALGKLELLVCQELFLTETAAMAHVVLPACSYMEKDGTFTNSEGH
                     VQAVRQAINPIGDSRPDWEMLSAISVLMGSPLEYGDAREILKEIRSVIPGYGLLGPTP
                     TSPKVDQPILSRYLAEDASTDMASRYALRQAPKSSETSLTLLFTQTLFHSGKLSTHSK
                     GLLQLQQEGFLSLNPVDAAKVGVADGEKVTVSNARGSITTIAKIRERVPAGLVWFPEH
                     FDGEAKQLAEWTIDPRTQIPYFKLAHVSLAKVS"
     gene            complement(7186..8493)
                     /gene="nuoF"
                     /locus_tag="PLS35_12980"
     CDS             complement(7186..8493)
                     /gene="nuoF"
                     /locus_tag="PLS35_12980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNI19629.1"
                     /translation="MKTYEPILLKNMIQPGYAGSLPEYERAGGYQALRKVLGKVTPAE
                     VTAVVMKSGLRGRGGAGFPTGVKWGFLPKDYQGPRYLCCNADESEPGTFKDRQLIERD
                     PHQLLEGMLLACYAIGAASSYIYIRGEFVLGAKILEAAIKEARAAGYVGKNILGTGVS
                     IDIWVHRGAGAYICGEETALLESLEGKRGLPRVKPPFPATHGLYNKPTVVNNVETLAN
                     LPHIMNRGAEWFASIGSPPKSTGTRVFCVSGHVARPGNYEVPMGITFRELIYEQAGGM
                     RSDKKLKAFIPGGASAPFLTPDHLDVKLDFESVALAGSMLGSGGVTVMEEGTDMVWAA
                     LRLMEFFYHESCGKCSPCREGSSWLVQIMRRIVNKRGRMEDLETLTDLCKNIAGRTVC
                     AFGDAEVSPILSTLKHWRHEYVTMIHAAEAANLIRPEPAGAKH"
     gene            complement(8560..9096)
                     /gene="nuoE"
                     /locus_tag="PLS35_12985"
     CDS             complement(8560..9096)
                     /gene="nuoE"
                     /locus_tag="PLS35_12985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNI19630.1"
                     /translation="MLKEKYQAEIDEILSRYPVKRSALLPLLYLAQREEGYVTEAAMQ
                     EIAGILKLTPPQVYETATFYTMLNLKPVGKFHLQVCKSLMCALVGSDTVIGWIGTKLG
                     IKPGETTQDKLFTLSIVECLAACGTGPMMQVNDDYYERLTEEKLDRILADLRQTGTCS
                     LKTGPFMWPEPAAAKSGA"
     gene            complement(9217..10971)
                     /gene="nuoD"
                     /locus_tag="PLS35_12990"
     CDS             complement(9217..10971)
                     /gene="nuoD"
                     /locus_tag="PLS35_12990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNI19631.1"
                     /translation="MQPLLERLMTTFPDAVRGVEVDAARNEVTARVSAARIVDVARWL
                     HDTPEAAFDHITDICSVDYPNDTERFEVIYQLLSLAHRRRIRLKVRITEDHPEVASVT
                     GIWKGADFMEREVYDLMGITFVGHPDLRRILLPEDYGEGHPLRKDFPAEGRGWRSEFP
                     FIPRLDEAPEELSAEGEIPESEKQVYRATEQPSSTRRREELLLNMGPQHPSTHGVLRV
                     VLELDGERIVKATPDLGYLHRGVEKLAEGLHYMQVIPHTDRLDYVCAMTNNYAYVRAV
                     EKLLDITVPERAEYIRTVVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREILLDM
                     FEKLCGARLTLNYYRIGGVDSDFTPDLVTRLKAFLHTFPDKVNEYNQLLMSNRIWVGR
                     TKDIAVISAEDAINFGLTGPTLRGSGVDYDIRKYEPYGAYDKVEWEVPVGKRGDTYDR
                     YWVRMEEMRQSARIIAQCLDQMPEGPIMADVPHVIPPPKAKVMRDMESLIHHFIIFTQ
                     GFKPPKGETYCGTEVPKGELGFFIVSDGSPRPYRLKIRAPSFIHMGAFDHMARGYLIS
                     DIITIFGTYDIVMGECDR"
     gene            complement(10987..>11465)
                     /locus_tag="PLS35_12995"
     CDS             complement(10987..>11465)
                     /locus_tag="PLS35_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNI19632.1"
                     /translation="SFLERQLDANILTTNLDAFVGWARKSSLWPMTFGLACCAIEMIA
                     SVSSRYDIDRFGAGVFRASPRQSDLMIVAGTVSRKMAPVIRRIYDQMAEPRYVISMGS
                     CATSGNHYNSYAVVQGVDQIVPVDVYIAGCPPRPEALIDGLLKLQQKIQREKIFVK"
BASE COUNT         2390 a         3337 c         3453 g         2285 t
ORIGIN      
        1 ccgctctgtg tcagacggat caaccagccg ccccaggcaa ccgcacgggc gactccattg
       61 acggcgccat cgatgatggc cacatcgaca tgtttccaca atccctgtgc ggccgacagc
      121 gtcggacgga cgaaggagcg gtcgtaggct tcatcgatat accacttgtg caacgacaac
      181 tcgtaggccg cccgccactg ttgcgcgagg cgatccggca accccggatt cagtacatac
      241 aggtagtagg ccgcgccgat tcccgtcagg cccatgagcg tcgccagcgc catgatgccc
      301 aaggcggcgc ttccttcgtg atgggcgcct gcgccttccc catgaaagac cggttccaga
      361 aacgccggga tgccgagata gcccgcgacg atactcagga cggccagcac catcaacggc
      421 gcggtcatcg tcgtcgacgg ctcgtgaaca tgccctgcat ggtgcggatc gacgtgggac
      481 ttgccccaga aggtcacgaa taccaaccgg aaactataga aggcggttag tccggccgtc
      541 accaatccgc aaatggtgag gacctgaccc agaggcccgg ccgaccagga ggagaccagc
      601 agatcgtcct tgctgaagaa gccggcggtc agcgggaacc cggccaatgc cagtgagccc
      661 acgaggaagg tccaataggt gaccggcagt ttgtctttca ggccgcccat gtgacgcatg
      721 tcctgttcgt gatgcagcgc gatgatgacc gagccgcagc ccaaaaagag aagtgccttg
      781 aaggccccat gggtgagcag atggtacatg ccggcgctgt aggctcctaa accgcaagcc
      841 atgaccatgt acccgagctg gctcatcgtc gaataagcga ccacacgttt gatgtccgtc
      901 tgggttaggg cgatggttgc gccgagcatc atcgtgagcg cgccgaccag ggcgacgacc
      961 gtcatggcgg tgggcgacag gttgtacagc ggggccaatc gcgcgaccat gaagacgcct
     1021 gccgtgacca tggtggcggc atggatcagg gccgaaatcg gcgtcggtcc ttccatcgca
     1081 tcgggaagcc agacatgcag cgggacttgc gcggatttgc cgacggctcc ggtaaagagc
     1141 agaaggcaga tcatcgtcat caccgaaaca tcccaggtgc cgccgaacgg gccgagaagg
     1201 ttcgttgtct tggacgccaa ttgccctgct tgcgcaaaga ccgtggcata gtcgagcgag
     1261 ccgaaggagt accagacgag gaacaggccg aggatgaacc caaaatcgcc gacgcggttg
     1321 acaaggaagg ccttcgttgc ggcagcgcag gcgctcgccc gttcatacca atggccgatc
     1381 aggagatagg agcagagtcc gaccgcctcc cagaacacga ataactgcag caggttgtcc
     1441 gccatcacca gcatcaacat cgagaaggtg aagagggcga tataagcgaa gaagcgggcg
     1501 tagccgggct cgccatgcat atagccaatc gtatagatgt gcaccagcgt gctgacggtg
     1561 gtgaccagga tcagcatgac ggcggtcagg cgatcgatgg aaatgccgat gtgaatatcg
     1621 agattgcccg aggtcagcca ggtgtacagg ttcactgtga tggcctggcc cgttgcgacg
     1681 tcgtgcaagg ccatgagcga caggaggaac gagcccacca tagccggaac ggcgacgaga
     1741 tgcgcgcggt ccttgatcca ctgttcgccg atgccgacga tcagaaagga gaacaagggt
     1801 agcaatggga tcagcgcgta catcatggtt ggtacgtttg gtctaccgtt tcaataaatt
     1861 caagtcgtcg atatagatgg acgaattggt ccggtgaagg gcgataatga tcgccagacc
     1921 caccgccact tccgccgccg ccacggtcaa ggcgaagaac acgaagacct gtcccgcgac
     1981 attgtgaaaa tattccgaaa aggccacgaa gttgatgttg gtggcattca gcatcagctc
     2041 gaccgacagc aagatcacaa tgatgttccg ccggatcagc acgcccacca cgccggtgag
     2101 aaacacaaac ccgctcagga ccagatagta ggataaggga acagtcatga cagtaccggt
     2161 tattgctgaa cctgttcgtt gatatctttt ttcgccagaa taatggcccc gatcatcgcg
     2221 accagcagga tcaacgaggc gacttcaaag ggaaacaaat aggttgagta caaggcttcc
     2281 ccgaccattt ccgtattgcc tgatcccgtg acggggtccg cggggaccgc ctgggtggaa
     2341 gcagccgtcc gtgattgaat cagcaacaag atgatttccg tgcacagcat cacgcccagg
     2401 agtcctgcga cgggcagttg gttgtgatag cgctcttcgg acttgatgct caggagcatg
     2461 acgacaaaca ggtagagcac cagaatggcg ccggcataga cgacgatctg gactgcggcc
     2521 aggaactccg cgtggagggt cacgtacagt cccgcgacat ggaagaacat gatcagcaac
     2581 gccagcgcgc tgtacaccgg gttgcgcaag gcaaccacca gcacggaagt ggtggcgatc
     2641 accgcagcaa aataaaagaa aaaaatgtga tccatgagtc cgttatctcg tccgaatcaa
     2701 ctgaaaccct gcccctgtcg ccctggctct ctgcccggtg gcagcctgtt tgcctccttc
     2761 gtctctgtgt ggggggccag cacggaggac gaggcggaag gcctagttct ccttttgcgg
     2821 gagatgcttg aacgtcacgt taaaaaacgc cacgttcggg tgctgcagct cgaggcgctt
     2881 ttcccggacg ggataggcgc gatcgccgat ggcgagcagc tgctgcttat tgagatggag
     2941 ttggcgcttg tcgtataccg cccattcgaa ttcgcgggtc atgcccaggg catccacggg
     3001 acaggcgtcg acgcagaggc cgcagaagag gcatcgcgtc atatccatgt agtattcctt
     3061 ggagtaccgc ttcgtcggtt ctcccggcac ttcaccgctg acgacccgga tcacacgcga
     3121 ggggcaggcg gcctcgcaca ggtcgcagcc gacgcacttt tccgttccgt cgtcataccg
     3181 caacaaggcc agcatcccgc gataattctc gggtaggagg cgcttctcat gcggatactg
     3241 tagcgtgatc ggcttgtaat tgagtaggtg cttcagggtg gccttcatgc ccacgagcag
     3301 ttcatagaag gtcagcgttt tcagccaatc gctgaacgac ggttttcgct taggctgtgc
     3361 cgtgatggcg acgttcatct cgctccgtta ccgcgggaag aaataggcgg cgatggccgt
     3421 caacacaatg ttgccgaggg cgatgggcaa catcaccttc catccgaacc gcatgagctg
     3481 gtcgtaccgc aggcggggca atgtggcccg caaccagaag aacaggaaca ggaagaagta
     3541 gacctttgcg gcgaaccaga cgacgttttc gatccaggcc agggcatcca gtccgaggtg
     3601 cccgagaatc gtgccggggt agggggcatt ccagccgccc aaaaagagcg ccgcggcgac
     3661 gcaggacacc aggatcatgt tcgcgtattc cgcaatgaag aaaaacgcga agcgcatccc
     3721 actgtattcc gtgaagaagc ccgccaccag ttcgctttcc gcttcgggaa ggtcgaaggg
     3781 cacacggttc gtttccgcta ccgacgaaat gacatagacg acgaaagcaa aaatctgggg
     3841 gaacggcatg gcgatgatga accagttcca aaaccctccg gcctgcgcct cggtgatctt
     3901 caccaggctc agcgagccgg acaggaggat gacgcccacg atcgcgagcc cgacgttcaa
     3961 ttcgtaactg atgacctgcg cggcggaacg cagtccgccg aggagggaat acttgctgtt
     4021 ggaggaccag ccgccgagga tgatgccgta ggcgccgata gaggcaaagg ccagaatata
     4081 gagaatgccg atgttgatat cgctgatgac gaacggcttg acctgcatgc cgaacagctc
     4141 gatcgtcatg gtcgggccga atggaatgac agcaaacccg atcatggcag gcaccagggc
     4201 gagaataggc gccaaggtaa agaggaactt gttcgccccg gccggaatga tgtcttcctt
     4261 gaagaacagt ttcaatccgt cggcgatcgg ttgaagcacg ccgtaggggc cgacttccat
     4321 cgggcccatg cgatcctgca tccagccgag cacttttcgc tctgcgaggg tcagaatcat
     4381 gacggtcagc atcaccactc ccattaccgc ggcgatttga gctaacgaca ccgccagtcg
     4441 taatccgatt tccataacag caatactcct cgtcccgtcc tacgagacct tcgccagaga
     4501 cacgtgcgcg agcttaaaat aggggatttg agtccgggga tcaatcgtcc attcagcgag
     4561 ttgtttggcc tcgccgtcga aatgttcagg aaaccacacc aggccggccg gcacacgctc
     4621 gcgaatcttc gcgattgtgg tgatggagcc gcgtgcgttc gagacggtca ctttttcgcc
     4681 gtccgccacc ccgactttgg ccgcatccac cggattgaga gacaagaacc cctcctgctg
     4741 caattgcagg agtcccttgg aatgggtgga cagtttgcct gagtggaaca gggtctgagt
     4801 gaacagcagc gtcaacgacg tctcgctgga tttcggcgcc tgtcgcagag cataccggct
     4861 tgccatgtcg gtcgaggcgt cttccgccag atagcggctc agtatgggct ggtccacctt
     4921 gggtgaagtg ggtgtcggtc ctagcaggcc ataaccaggg atgacgctgc gaatttcttt
     4981 cagaatctcc cgcgcatcgc catattccag tggcgagccc atcaagacgg aaatcgcgga
     5041 caacatttcc caatcggggc gactgtcgcc gatcggattg atcgcctgac gaacggcttg
     5101 cacatgcccc tcggaattgg tgaaggtgcc gtccttttcc atataggagc aggcgggaag
     5161 cactacgtgg gccatcgctg ctgtttccgt gagaaagagt tcctgacaga ccagcagttc
     5221 cagtttgccg agagcctctt tcgccttcgc cccggtcggc aaggtgccaa tcggattctc
     5281 tcccaccaca aacatggcct tcagagagcc gctattggcg gcggcaagca tctcggtcaa
     5341 cgagatgccg gctgtgcggg gaagctcctc gcgccacagc gatgccagac ggtccctcgc
     5401 ggcttggtca ttgagcgggg ccggtccggg aaggaactca ggcacggcgc ccatttcgac
     5461 tgcgccttga tcgttgtttt cttcggccag cggtccaagt ccgcacccag gttgattgag
     5521 tttgcccagc aggatcagga gatccaggag attcgtggtc gtgccttctc cgccgggatg
     5581 gcgcaggacg ccgttgccca cgaggatcac gacgcgtttg gccgcggcca gaagtttggc
     5641 ggtctccgcc cattgtgcgc gggtatggcc ggtggcggtt tcgagtgtgt cccaggaaat
     5701 ctgttgcgta gcggcggata gccgttgcac gaaggccggc gattgctcag ccaggcctgc
     5761 gtccaccagg ttgccatcaa tgacggcctt gatgaggccc aagacgttat taccaatctg
     5821 cgcaggatgg gcagagaaat gttgcgtggc caggttcgcg atattgctca atgtgtcgac
     5881 ggccggttcc agcgactcaa tcgtgaccag actcgcctgg cgcttcttca cggcttcttt
     5941 gaccttgagg ccggtaatag ggctggtctc cgtgatgttg gtgccgacca gcagtaaggc
     6001 gttggcggcc acaatatctt cgaacgcgat ggtccagcgg tgggtccctt gcacccgttg
     6061 tagtgctcgc accccgctca gatggccata ccgcgcgctg ctatcgattc gattggtgcc
     6121 gatcacctgg cgcatgaact tctggaatac gtaaaggtct tcgttggtgc agcgggagct
     6181 gatgagccct ccgaacgcat ccgggccgtt ggccagcttc acccggaggg cctgttcggc
     6241 gacatattcc aatgcgtctt cccacgtggc ttgcaccaag acgccgtcgc gtcggatcaa
     6301 gggatgggtc aggcgatcgg catgggtgct tgcatggaac ccgaagaagc cgcgggcgca
     6361 gaggtcgcca ttgttccgtc cggcgccgtg cgacgaattc acttcaatga gttcgtttcc
     6421 tttggtctgg accgtgatgc ggcagccgtc gccgcagaag gtgcagatcg tatcggcccg
     6481 cttgagcatc cagggccgat attcatacat ggacagccgg ctcgtgatgg cgccgaccgg
     6541 gcagatctgg atgcagccgc cgcagaattc gcagtcgagt tcatgcgggc cgaaatgttt
     6601 gatttccgtc atcgtcccgc ggccgacggg cgccagggcc ttgacgtcca tgatttcgtc
     6661 gcaatagcgg acgcagcgca aacactgcac gcaacggttc atctgcgttt cgatgagcgg
     6721 gctgaagtac tctttctgaa agatgcgctt ggtttccgtg aaccggctgg tcgtcggtgt
     6781 gtattgatgc gagaagtctt gaaggtcgca cttgccgccc tggtcgcaga ccggacaatc
     6841 gagcggatgg ttcgccagaa tgaattccag caccgacttg tgcgcatcgt ccaccgtggt
     6901 ggtggcggtt cgcacggcca tgccttcggc caccggcgtg ctgcaggcag tttgcagctt
     6961 cggcatcttt tcgacttcga ccaggcacat gcggcagttc gcatccggct tcaacttggg
     7021 gtgatagcag aaatgcggaa tcatcacccc cacgcgacgc gcggcttcaa tcacgagcgt
     7081 gcctttggga acggcgacgg tggtgccgtc gatggtcagc cgaacggttt ctggtttttg
     7141 atcaggcata gtggccgtta ttcgtgcaac gtgacacgag agacgtcaat gtttcgctcc
     7201 tgccggttcc ggccggatca agttggcggc ctcagccgca tgaatcatgg tgacgtactc
     7261 gtgccgccaa tgtttgagtg tgctcaaaat cggagacacc tcggcgtcac cgaatgcgca
     7321 gacagtccgg ccggcgatgt ttttacacag gtcggtcagg gtttcaaggt cttccatccg
     7381 cccgcgtttg ttgacgatcc ggcgcatgat ctgaacgagc caggaactgc cttcccggca
     7441 gggactgcac ttgccgcagg actcatgata gaaaaattcc atcaggcgca gcgcagccca
     7501 gaccatatcg gtgccctctt ccatgactgt cacaccgccc gaccccagca tcgatccggc
     7561 cagcgccacc gattcgaaat ccagtttgac gtccaggtga tcgggtgtga gaaagggcgc
     7621 ggaagcgcct cctggaataa aggccttgag tttcttgtcc gaccgcatgc cgccggcctg
     7681 ttcatagatt aactcgcgaa acgtaatgcc catcggcact tcgtagttgc cgggtcgcgc
     7741 cacgtgaccg ctcacgcaaa agactcgtgt gccggtgctt ttcggcggag acccgatcga
     7801 ggcaaaccat tcggcgccgc gattcatgat atgcggcaga ttggcgaggg tttcgacgtt
     7861 gttcacgacc gtcggtttat tgtagagacc gtgcgtggcc gggaatggcg gcttgacgcg
     7921 aggaagaccg cgtttgcctt ccaacgattc cagcagggcc gtctcttcac cgcaaatata
     7981 ggcgccggct ccgcgatgga cccagatgtc gatcgagaca ccggtgccca ggatattctt
     8041 gccgacatag cctgcggcgc gcgcttcctt gatggcggct tcgagaatct tggctcccag
     8101 gacaaactca ccgcgaatat agatgtagga actggctgcg ccgatggcgt agcaggccaa
     8161 caacatgcct tcgagcaatt gatgcggatc gcgctcaatc agctggcgat ctttgaacgt
     8221 ccccggctcg ctctcgtcgg cattgcaaca gaggtaacgg ggcccctgat aatccttggg
     8281 caaaaatccc cacttcacgc cggttgggaa tccggctccg ccgcgtccgc gcaatccaga
     8341 cttcatgacg acggcggtca cctcggcggg ggtgactttt cccagcacct tccgcaacgc
     8401 ctggtatccg cccgcgcgct catattccgg cagcgagccg gcataccccg gctgaatcat
     8461 gttcttgagc agaatcggtt cgtacgtttt catacaggcc atctcatgga acgtgacacg
     8521 cgagcagtct cctttgagcg aatcgcaaac gagggcgggt taggcccccg actttgccgc
     8581 ggccggttcc ggccacatga acggcccggt tttcagcgag caggtgcctg tctgccgcaa
     8641 atccgccaga atgcggtcta atttctcttc cgtcagtcgc tcgtaataat cgtcattcac
     8701 ttgcatcatc gggccggtcc cgcacgcggc caggcattcg acgatactga gcgtgaacag
     8761 tttgtcctgg gtcgtttcgc ccggcttaat gccgagcttg gtgccgatcc acccgatgac
     8821 ggtatctgag ccgaccaggg cgcacatcaa cgatttgcag acctgcaggt ggaactttcc
     8881 caccggcttc aggttcagca tcgtgtaaaa cgtggccgtc tcatacacct gcggcggggt
     8941 cagcttcaag atgcccgcaa tttcctgcat ggcggcctcg gtgacataac cctcttcgcg
     9001 ctgcgccaag tagagcaggg gcaagagcgc ggatcgcttc accggataac gagacaggat
     9061 ctcgtcgatt tccgcctgat atttttcttt cagcatctct ctcactccac gctgccagtt
     9121 acggtgagtt ccacgtcggg ggtactcggt gcgtccaacg ggggtctgct cagaccgcgc
     9181 attccgcgag cactccggac acctggctca tgcgcctcat cggtcacatt ctcccatcac
     9241 gatgtcgtag gtgccgaaga tcgtgatgat gtctgaaatc agatatccgc gcgccatatg
     9301 gtcgaatgcg cccatgtgaa tgaaggaggg ggcgcgaatc ttgagccgat aggggcgggg
     9361 gctgccgtcg ctgacgatga aaaacccgag ctcgcctttc gggacttcag tgccgcagta
     9421 ggtttcgcct ttcggcggct tgaaaccctg ggtgaaaata ataaagtggt gaatcaggct
     9481 ctccatgtcg cgcatgacct tcgccttggg cggagggatc acatgcggca catccgccat
     9541 gatcgggcct tccggcatct gatccagaca ctgggcgatg atccgggcac tctgccgcat
     9601 ttcctccatg cggacccagt agcggtcgta ggtatcgccc cgcttgccga cgggcacttc
     9661 ccattcgacc ttgtcgtagg cgccgtaggg ctcatacttg cggatgtcgt agtcgacgcc
     9721 ggacccgcgc agtgtcgggc cggtgaggcc gaaattgatg gcgtcttcgg ctgagatcac
     9781 cgcgatgtct ttcgtgcgcc cgacccagat gcggttggac atcagcagct gattgtattc
     9841 attgaccttg tccggaaacg tgtgcaggaa ggccttcagg cgcgtgacca gatcgggcgt
     9901 gaaatcgctg tcgactccgc cgatgcggta atagttcagc gtcagacgcg cgccgcacaa
     9961 cttctcgaac atgtccagaa ggatttcgcg ctcgcgaaac gtccaaaaga agacggtcat
    10021 ggcgccgatg tcgagcgcct gggtgccgag ccagaacaga tggccgatga tgcgctgcat
    10081 ctcggcgacg acggtgcgga tatactccgc ccgttccggt accgtgatgt cgagcagctt
    10141 ttccacggcg cgcacatagg cgtagttatt cgtcatcgcg cagacgtaat cgagccggtc
    10201 ggtgtggggg atgacctgca tgtagtggag gccttccgcc agcttttcga cgccgcgatg
    10261 gagatagccg agatcgggag tggctttgac gatccgctcg ccatccagtt ccaacaccac
    10321 ccgtagcaca ccgtgtgtgc tgggatgctg ggggcccatg ttgagcagga gttcttcccg
    10381 ccgccgtgtg ctgctcggct gttcggttgc ccgatagact tgtttttccg attcggggat
    10441 ttctccctct gccgacagtt cttcgggggc ttcgtccaac cgtgggatga aggggaattc
    10501 gctccgccag ccgcgccctt ccgcgggaaa atctttccgc aaagggtggc cttctccata
    10561 gtcttccggc agcaaaatgc gcctgaggtc cgggtgcccg acgaaggtaa tccccatcag
    10621 gtcgtagact tcacgctcca tgaaatccgc gcccttccaa atgccggtca cggaggcgac
    10681 ttccggatgg tcttcggtaa tgcgcacttt gagtctgatg cgccgccgat gtgcgagtga
    10741 taacaactgg taaatgactt caaaccgttc cgtatcgttc ggatagtcta ccgagcaaat
    10801 atcggtgatg tgatcgaacg cggcttcggg tgtgtcgtgc agccagcgcg cgacgtcgac
    10861 gatccgcgcc gcggacacac gcgcggtcac ttcgtttcga gcggcatcca cctccacgcc
    10921 gcggacggcg tcgggaaagg tggtcatgag tcgttcgagc agcggttgca tgatgaaagg
    10981 agccggttac tttacgaaaa tcttttcgcg ctgaattttt tgctgcaact ttaagagccc
    11041 gtcgatcaac gcctccggtc gaggagggca gccggcaata tacacatcga ccggcacgat
    11101 ctggtcgacg ccctgtacga cggcataact gttgtaatgg ttgccggagg tcgcgcagga
    11161 ccccatggaa atgacgtagc gcggctcggc catctggtcg taaatgcgac gaatgacggg
    11221 agccatcttc cgggataccg tgccggccac gatcatgaga tccgattggc ggggcgaggc
    11281 ccggaacacg ccggcgccga accgatcgat gtcatagcgt gaagacacgc tggcgatcat
    11341 ttcgatggcg caacaggcca acccgaacgt catcggccac aaggacgatt tgcgcgccca
    11401 gccgacgaag gcatcgagat tcgtcgtcag aatatttgca tccagctgcc gctctaaaaa
    11461 gctca
//
LOCUS       DAOQEW010000122        11364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1723_length_11364_cov_8.801397, whole genome shotgun sequence.
ACCESSION   DAOQEW010000122 DAOQEW010000000
VERSION     DAOQEW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11364
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_11364_cov_8.801397"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..2074)
                     /locus_tag="PLS35_13000"
     CDS             complement(68..2074)
                     /locus_tag="PLS35_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNI19633.1"
                     /translation="MKTALIVLWMLLSILGAVALAHVVGVINPTEKVNGLWLVVAAAC
                     IYVLAYRFYGRWLARKVVQLDDRRLTPAVRLNDGVNFHPTNRVVLFGHHFAAIAGAGP
                     LLGPVLAAQFGFLPGFLWLVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHDELGSIT
                     GTATAVAVLFIVVVALAGLGFAVVNALYHNAWGTFTIAMTIPIGFIMGFYLQKFRPGA
                     VAEVSVLGLVLLVTAVLFGRVVGQSPYAWLFEFEKPALVWLLAGYGFVASVLPGWMLL
                     VPRGYLSTFMKLGVVFLLGLGVILMAPTIEMPRLTAFANGGGPIIPGTLFPFLFITIA
                     CGAVSGFHSLVSSGTTPKMIEQESQAVVGYAAMLLESFVGVMALIAASVLIPGDYLAI
                     NTMLSAETLTAMGFAPSRLAELSQLVEVNVAGRPGGAVSLAVGMASIFSALPGMAGLM
                     AYWYQFALVFEALFILTTIDTGTRVARYLIQEMAGRVYAPFRQMNWWPGVLLSSAFVV
                     GSWAYLIGTGSISTIWPMFGAANQLLGTLALCIATTVLIKMWKSPYLWVTAIPMLFVG
                     GITLAGSYEMFWMFLAKAATLASGQAFALYLDAVLVAVVAILGVVVLSDSMKQWYGYV
                     ILKRPFSSSEVIATAGGGSAGRAQTAIHRHDDRKFTLPHGGGCC"
     gene            2216..2611
                     /locus_tag="PLS35_13005"
     CDS             2216..2611
                     /locus_tag="PLS35_13005"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI19634.1"
                     /translation="MSPATPSEPSGRERREFYRITVLLPMCLQMAGDETEGELIETSV
                     NLSAGGIGVMVTTPYKSGDVLSLTLLLPDQVRFKASIDVLRLDPLPLLGGVYRLHARF
                     VGLPAHDRELLVRYILRFQRDHLQEHYSA"
     gene            2716..3723
                     /locus_tag="PLS35_13010"
     CDS             2716..3723
                     /locus_tag="PLS35_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI19635.1"
                     /translation="MRMLTWLLGTIVALATIGAVYQIAGMVLDRQHYPPPGQLVDIGG
                     HRLHLFCLGTGSPTVLLEAPAPGWSLYWSTVQPQLARTTRVCAYDRAGLGWSERGPLP
                     RTGQRMAKELHRLLERAGIRGPYILVGHSLGGLIARLYQHDYPQEVVGMVLVDAGHES
                     EMRQAEFRLFANAGKSMLPVIRALTMVGITRLMASYDQLPPLLMRQEEKVSAETRPML
                     RAGWLRTGYYSTLTDESDGLIETLEQVRRSGSLGNLPLIVITATGPVWWPDMPGQVNQ
                     AKFRKMWLELQQGLTTLSTNSRQVFADQSSHFIPFDQPELLTDVVRQLIDRQRSAPYR
                     P"
     gene            complement(4477..4914)
                     /locus_tag="PLS35_13015"
     CDS             complement(4477..4914)
                     /locus_tag="PLS35_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNI19636.1"
                     /translation="MTSYHAALVAPIGQDSFDRQIDRLLEDAVSAFGVSGIAWAPASN
                     AWEDDNGFYVQVALPGWEPQDVSLEVAKHVLSIKGERKEQPQPGKTVHVQEIGDGRFM
                     RLFRLPTYIDSEKASAVQKNGLLTVTFPKREEAKSRRIMIEGT"
     gene            complement(5162..6730)
                     /locus_tag="PLS35_13020"
     CDS             complement(5162..6730)
                     /locus_tag="PLS35_13020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNI19637.1"
                     /translation="MRLTDPSPIGQRNEALWGTDLVVAACLFLMVFGSGVVHIQSFPP
                     LFFDEGWTVCVARTWVESGHYGCLLRGEPAPPSLAAHFPVVASVAASFALFGVGVWQT
                     RVVGLLYTFAALLLLYAVARRLFGRSVAIAALALLILVPLKWSIHPLYLGRQVLGEMP
                     LLCFLLAAFVCFLRSAHRPLWLVAAIGCWSLAFMTKAQVAPFLIASFVGTIVILTLRR
                     NWSAVRQLALVLIGSWAGFRFLIEAKNWLLSGHTMAHPSTDGMTEAIALVFVPAIRLE
                     TIQYLFVAWAEYPLGLAYAAWRLHRTPASRDEESVAQTAQIMLLLLAGSWLAWFAFLS
                     AGEPRYALPGLFLSAPFAALFVCEATRGFDVRYLLRTLAELVPGRRVTPQGLKAAVVV
                     VLLAIMGWGAVQERYAFRAREDDRDLFAVTQHLNTTTSPTALIETYDSELFLFLNRPY
                     TYALPKHLVEIIRREQGQAQAVTYDPLQQNPDYLVVGEYGRWAGFYKPLIAQNRVRLV
                     TTIGRYQIYEPVRS"
     gene            complement(6886..8220)
                     /locus_tag="PLS35_13025"
     CDS             complement(6886..8220)
                     /locus_tag="PLS35_13025"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HNI19638.1"
                     /translation="MSDVQLRTKAWFGDEPLSINFPPAWNIVEVGPQDAPALSATAMR
                     EKLNVPIGSPPLREIAKGKTKCVIIVDDLTRPTPAADLLPSIIAEIQAAGLSEHAITI
                     LLAGGTHPPASAQEIEKKVGANLSPAVRVMAHDSRGALVDLGMSPGGLPIRINRTVME
                     CDLKIGVGCIYPHPIAGFSGGAKIVLPAVCGVETTRMMHDYLRGSRTRGGSIHTELRQ
                     DMVAVARKAGLDFIANVTLNHRREISGLFAGDVVKAHEEGVRAAQRMYAVPVVSEADI
                     VVADMYPFDTSWQFAQDRGMWPVECAGSQASRVVIAACPLGLGTHELFPVDSPLWSRV
                     ARRLKHFRLADLDRPFEKLGTIMKLLRQKQHHLMVLSPGLEGKDLHTMFPHAQRFGAW
                     PPLRAALVARHGEGPLTVAVYRCAPLLIPAALDLEQEIGVPDPRATVRLTSA"
     gene            complement(8217..9101)
                     /locus_tag="PLS35_13030"
     CDS             complement(8217..9101)
                     /locus_tag="PLS35_13030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI19639.1"
                     /translation="MIPTLGRETLALCQEGLSKQTRLPDEVIVVLDRDRRGVAWGRNE
                     GIARSTGDLIAFVDDDAVPPQDWLERLVTAIDHYDAAVAGGTFKETDPLLDAIRRLNS
                     LPTVEQLDPGGLVGNAGNILFRRDCLLKCEREDGYVFNPLFTGAGEDWELIWRLRKRG
                     AKMVFVPNPVTHLRRATGLQHLRHSFHRGAGIAILFRVMRRDSSGVVPQDSLLWGEGG
                     KKTKPRWLKAIWVKMLGPFEWQKFQRKRHFCLFWLGEKCQAAGFFWEMCRKRSMTHID
                     RKTKPMVQAVSSRVESKS"
     gene            complement(9149..10693)
                     /locus_tag="PLS35_13035"
     CDS             complement(9149..10693)
                     /locus_tag="PLS35_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNI19640.1"
                     /translation="MSAKAVSKGILSVGGWSMAKLATSAVVLPILARFLGIEGYGQYA
                     YYLAVLLLASQFANVGMMQTMTRRIAERPDDAAWCRAVARSGALINGTGVVFVGLAAG
                     VLIWGTASHETARVPIALAVVGVLVFDQVWFYARGVLHGLRHEERAAVPGIMGVLMAG
                     SFGIIAAVTGMGVVGVLVGLLVADIFVAGACLRAVMVALNGLGLPASQGPPALPTGAV
                     LRFGLSAMIFSVLNMALCSLDIILVRHLAGEAQAGLYAAAVQWSQFVWFIPIAVEGVM
                     LQATARLWAEQRVEAVTSLVSRLVRYVMLGTVFLLIVVFVLGDRIITVYFGSQFSEAA
                     LGLRLLVPGALCYALARVLWPVIQAGGEAMSLIRVMLLTVMVDAGLCAILIPAGGAAG
                     AACATSLSFALVAGGYLWILRRRNVSVFEGLALSRLVGLVVMTGIAMAGLSALVATPM
                     LSLLVGIAVGVFLYWGGVFSLGIVQVDELESLVQSLPGTFRKAGESVLRMVAPLLLRL
                     KAAASN"
     gene            complement(10755..>11364)
                     /locus_tag="PLS35_13040"
     CDS             complement(10755..>11364)
                     /locus_tag="PLS35_13040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI19641.1"
                     /translation="KRLYGLKQLPTLLPNLIDVPSALPRKSERPTITFIARWDKRKRP
                     WLFLELAAQFPDYRFVAVGKGSASAETGFDAELRQRFRNVPNLEMPGLINRFHEPERM
                     HQILSDTWVFVSTAVREGLPLTFLEAAAYGCPIISRVDPDQFATRFGKQVHDDDYAAA
                     LQSLLAEAPLEKGRAAYHYVRDTYETSRALIAHQEQYARFAA"
BASE COUNT         2608 a         3637 c         3079 g         2040 t
ORIGIN      
        1 tcacttccga caccacatcg aatgatgatt ggtcagccac gatgtcctcc ttctacgtct
       61 ctctatccta acaacaaccg ccgccatggg gcagcgtgaa tttgcggtca tcatgccggt
      121 gaatggccgt ctgcgcacgg cctgcggatc cgccgccggc ggtcgcgatc acttcactgc
      181 tgctgaacgg gcgtttgaga atgacatacc cataccactg cttcatgcta tcgctcaaga
      241 cgaccacgcc tagaatagcc accacggcca ccagcacggc atcgagatag agcgcgaacg
      301 cctgccccga cgccagcgtc gcggccttcg ccagaaacat ccagaacatc tcataggagc
      361 cggccagcgt aatgcctccc acgaacaaca tagggatggc cgtcacccag agatagggcg
      421 acttccacat tttgatcaac accgtcgtgg cgatacagag ggccagcgtg cccagcaact
      481 ggttcgccgc accgaacatc ggccagatgg tggagatgct gccggtcccg atcagatagg
      541 cccaggatcc caccacaaaa gcactgctca gcaacacgcc gggccaccaa ttcatttgcc
      601 gaaaaggcgc gtacacacgc ccggccattt cctgaatcag ataccgcgcg acacgtgttc
      661 cggtatcaat cgtcgtcagg atgaaaagcg cctcgaagac cagcgcgaac tgataccaat
      721 aggccatcaa gccggccatg cccggcaacg ctgaaaagat ggaggccatg cccacagcca
      781 gcgatacggc cccgcccggc cggcccgcca cattcacctc gaccaactgc gacaattccg
      841 ctaggcgcga tggcgcaaac cccatcgccg tcagcgtctc tgcagagagc atcgtgttga
      901 tcgccaggta atcaccgggg atcaacaccg aggccgcgat caatgccatc accccgacga
      961 aactttccag caacatcgcc gcatatccga ccaccgcctg cgactcctgt tcgatcatct
     1021 tgggagtcgt tcccgaagac accagcgaat ggaagcccga tacggcgcca cacgcgatcg
     1081 taatgaacaa aaaaggaaac agcgtcccgg gaataatggg tcccccacca ttggcaaatg
     1141 cggtcaggcg cggcatctca atggtcggcg ccatgagaat cacgccaagc ccgagcagaa
     1201 acaccacacc cagtttcatg aaggtcgaga gatagcctcg cggcaccagc agcatccacc
     1261 ccggcaagac cgaggctacg aatccatacc cggccaatag ccagaccagc gccggcttct
     1321 cgaattcaaa caaccaggca tagggcgatt gtccgacgac gcggccgaat aacactgccg
     1381 tcacgagcaa caccaggccg agcaccgaca cttccgcgac ggcgcccggg cgaaatttct
     1441 ggagataaaa tcccatgatg aacccgatgg gaatcgtcat ggcgatggta aaggtgcccc
     1501 acgcgttatg gtagagcgca ttcacgaccg caaaccccag ccccgccagc gcaaccacca
     1561 caataaacag gaccgccacc gccgtcgcgg tgccggttat ggagcccagt tcatcatgcg
     1621 cgatttccgg taacgagcgg ccgttgcgcc gcatcgaggc gaccaggata ataaaatcct
     1681 gcacggcccc ggccagaacc gcaccgatca ccagccagag aaagccgggg agaaaaccga
     1741 attgcgccgc cagcacaggt cccagcaagg ggcctgcccc tgcaatggcc gcgaagtgat
     1801 gaccgaacag gacgactctg ttggtggggt gaaaattcac cccgtcgttc aaccgcacgg
     1861 ccggcgttag acgcctatca tccaattgca ccactttccg ggccaaccac cggccataga
     1921 accgataggc caggacatag atgcaggccg ccgccaccac cagccacaga ccgttcactt
     1981 tttcggtggg gttgatgaca ccgaccacat gagccagcgc cacagctccc agaatcgaca
     2041 gcagcatcca gagcacaatt aacgcagttt tcatgcgcgg gatagtagca caaacacaag
     2101 gagatggggc gacgtgaggc cgtacgtgcg ggggccggac catgtagggg aacaggaagt
     2161 gcgggacggt tgagcgagaa cctgttcacc gggtttgtta tactttttct gaaccatgtc
     2221 gcctgccacg ccttcagagc catccgggcg agagcgccgc gagttttatc gcatcaccgt
     2281 cctgctgccc atgtgccttc agatggcagg agacgagacc gaaggtgagt tgattgagac
     2341 atccgtcaac ctcagtgccg gcggcatcgg ggtgatggtc accacaccct acaaatccgg
     2401 tgacgtgctc tctcttacgc ttctcctccc agatcaggtt cgctttaaag cgtcaatcga
     2461 tgtgttacgg cttgatccgc ttccgctcct cggcggggta tatcgcctcc acgctcgctt
     2521 cgtcgggctg ccggctcacg accgggaact cctcgtcaga tacattcttc gttttcagcg
     2581 cgaccatctg caggaacact attccgcctg actgtcccaa tgccctcact cgcgcggtac
     2641 tcagacatcc acggacattg atggatgaac tttccccgtt tgcttccgtg acctgtaaca
     2701 ggggatgatg cccgcatgcg catgctcacg tggcttctcg ggacaatcgt tgcactcgcc
     2761 actatcggtg ccgtgtatca gattgccggc atggtgctgg atcggcagca ttatcctcca
     2821 cccggccaac tggtcgatat tgggggacat cggctgcacc tcttttgtct tgggaccggg
     2881 agtccgacgg tactcctcga ggcgcctgca cctggctggt cgctttattg gagcaccgtg
     2941 caaccgcagc tcgcccgaac gactcgcgtc tgcgcctatg atcgagcggg cctcggctgg
     3001 agcgagcgcg gtcccttgcc tcgcacggga caacgaatgg cgaaagaact ccatcgtctg
     3061 ctcgaacgag ccggcatccg ggggccttat attctggtag gacattcgct cggcggcctg
     3121 atcgccaggc tctaccaaca tgactatccc caagaagtcg tcggcatggt gctggtggat
     3181 gcgggtcatg aatcggaaat gcgtcaggca gaatttcgat tgttcgccaa cgccggcaaa
     3241 tccatgctgc ccgtcattcg cgccctgaca atggtaggca tcacaaggct gatggcctcc
     3301 tatgaccagc tgccgccgct cttgatgaga caggaagaga aggtatcggc cgagactcgc
     3361 ccgatgctgc gcgccggctg gctacggacc ggctactatt ccacgctgac ggatgagagt
     3421 gacggattga ttgaaacgct ggagcaggtg cgccgcagcg gttcgctcgg caatctcccg
     3481 ttgatcgtga tcaccgccac tggcccagtc tggtggccgg acatgccggg acaggtgaac
     3541 caggccaagt tcaggaaaat gtggttagaa ctgcaacagg gactgaccac cctgtcgaca
     3601 aacagccggc aggtattcgc cgaccagagc agccacttta ttccttttga ccagccggaa
     3661 cttctcaccg atgtcgttcg tcaactgatc gaccggcagc gcagcgcccc gtaccgtccc
     3721 tgaggggctc ttccccagca gatcagcgag atccccgggg aagacttcat tacgcctctt
     3781 gtccaaggca tacagcgtgg cctgtgaata atcacgcagc cttaggcagc tggacgttat
     3841 ccctatatgc tctcatcgga accgccggct gctgggaggt agacacggtg ctggactcag
     3901 aagacagcat ccagagtccc atcaagagca ccgcgatcgc acccgccacc gttagtccgg
     3961 catcaagaac cgaactcaaa tccatcatcg tgttcacctc ctttctgttc cagatagtca
     4021 tcttgggatc acaaagcagg ctctcagggc gcccacactt aaaataggcc gtgcgaccac
     4081 ggggtcacgg gacgatcctg atgatcttct cttcgctcct gccgctcctg ggcccggcgc
     4141 ctccgccacc acccgcgtag ggaggccagt gacatcgcgc cctgcaaccc atgcgtaccc
     4201 aattccgagc ggctacctat gcaccgccaa cgcttcaaga aatttctgta gttcataggt
     4261 cttctccttg agcatttcac aatgccgcac atctcttctg cggcaggtca aactacctag
     4321 ccatgatggc ctccggcgtg agagtagagg cctcgccgat cgtgggaatc agcgaggcct
     4381 ccgggaacaa ccggttattc ggaggaaggc gggaggggaa ccgtctgagg atcccttcgc
     4441 ttcatcatgc cttctgcgca agacggatgg gagtgtttag gtcccttcaa tcataatccg
     4501 ccgactcttt gcttcttccc gcttgggaaa cgtcacggtc agcaagccat tcttttgcac
     4561 agccgaagcc ttctcggaat caatatacgt cggcaatcgg aagagccgca taaaccgccc
     4621 gtcaccgatc tcttgcacat gaacggtctt acccggttga ggctgttcct tgcgctcgcc
     4681 cttgatcgag agtacatgct tagccacttc gagcgagacg tcttgcggct cccaaccggg
     4741 gagcgcgacc tgtacgtaga acccgttgtc atcttcccaa gcattactgg ccggagccca
     4801 ggctattccg cttacgccga acgcgcttac cgcatcctcc agtaaccgat cgatttgccg
     4861 gtcgaaggag tcttgcccga taggtgctac caatgctgca tgataactgg tcataccgtc
     4921 ctcctcctct ctcctgagag gtcagtgtaa tggttgatgt gagtgggttt ggatggaatg
     4981 ctggccgaag cagaagcggg gcattcacct gccgccattg ccctccagac cgggcactgt
     5041 gtcccgtcca tccggttacg cattagcaac gatgaaaaaa taatcccgtc tcccatgaag
     5101 tcaagggggc aatttcacgg ctacagaaga agggaaaagg accgaggggg atacggtcgc
     5161 gctaggaacg caccggttcg tagatctgat agcgtccgat ggtcgtcacg agccgcacac
     5221 gattttgcgc aatcaacggc ttgtagaatc ctgcccatcg gccatattcg ccgactacga
     5281 gatagtccgg gttctgttgc agagggtcat acgtgactgc ctgcgcctgt ccctgttcac
     5341 gccggataat ctcaaccaga tgcttgggca aggcgtaggt gtagggacga ttcaaaaaga
     5401 ggaacaactc gctgtcatag gtttcgatca acgccgtcgg tgaggtcgtc gtgttcagat
     5461 gctgcgtcac cgcaaacagg tcgcgatcgt cctctcgcgc tcgaaaggca tatcgttcct
     5521 gcacggcacc ccaccccatg atcgccagaa gcacaaccac gacggcggcc ttgagtccct
     5581 gaggcgtcac tcgccgaccc ggcacgagtt cagccagagt ccttaacaga taccgcacat
     5641 cgaatcctct ggtggcctcg caaacaaaca aggctgcaaa cggcgccgaa agaaataatc
     5701 cgggcaaagc gtaacgcggt tctccggcgc tcagaaacgc aaaccaggcc agccaactcc
     5761 ctgccagcag caacagcatg atctgcgccg tctgtgccac ggactcctca tcgcgggaag
     5821 caggcgttcg atgaagccgc caggcggcat aggccagtcc caacggatat tcagcccatg
     5881 ccacgaacag gtactggatc gtctccaggc ggatggccgg cacgaatacc aaggcaatgg
     5941 cttctgtcat tccatcggtg gagggatgcg ccatcgtgtg cccgcttaac agccagttct
     6001 tggcttcgat caagaaccga aatccggccc atgaaccgat caaaacaagg gccagttggc
     6061 gcacagccga ccagttcctg cgaagagtga gaatgacgat ggtccccacg aaagaggcga
     6121 tcagaaatgg ggccacctgc gccttagtca tgaaggccaa cgaccagcac ccaattgcag
     6181 cgaccagcca gagaggccga tgcgcgcttc ggagaaagca gacgaaggca gccaacagaa
     6241 aacagagtaa cggcatctcg cccagcacct ggcgccccag atacagggga tggatcgacc
     6301 atttcagcgg cacgaggatc agcagtgcca gggcggcaat tgcgaccgat cgtccaaaga
     6361 ggcgacgagc taccgcatag agcagcagca acgccgcgaa ggtgtacagc agccccacca
     6421 ccctcgtctg ccacactccg acaccgaaca gggcaaaact cgccgcgacc gacgccacga
     6481 cagggaaatg cgccgccaat gaaggcggag ccggttctcc ccggagcaga cagccgtagt
     6541 gacctgactc gacccatgtc cgcgcgacac agacggtcca cccttcgtca aaaaacagtg
     6601 gagggaaact ctggatatgg acgaccccgc tgccgaagac cattagaaac aaacatgcag
     6661 ccaccacgag gtctgtgccc cacaaagcct cattgcgctg gccgattgga gacggatcag
     6721 tcaatcgcac tcgggtatcc ccagacccgg ttgttccgga aggcgcatca tcgtcgtcgt
     6781 gggggagcaa tggatgaggt gaggtcgttg gcatagcaga tgacaattca cccataaacc
     6841 aatcgtcgaa tcattgcagg acaggacacc ctccggtcct cgccttcagg ccgaagtcag
     6901 tcgcacagtg gcacgtggat ccggcacgcc aatctcctgc tccagatcca atgcagctgg
     6961 aatgagaagc ggtgcacatc gatagactgc caccgtcaaa ggcccctcgc catggcgcgc
     7021 cactaacgcg gcccgcaatg gcggccacgc tccgaatcgc tgtgcatgag ggaacatcgt
     7081 gtgtaaatcc tttccctcca gaccgggcga caacaccatc aagtgatgtt gcttttgacg
     7141 aagcagtttc atgatggtgc ccagtttttc gaacggacga tccaaatcag ccaagcgaaa
     7201 atgtttgaga cgtctcgcca cgcgggacca gagcggactg tcgaccggaa acaattcatg
     7261 cgtccccagc cccaaaggac aggcggcaat aaccacgcgg gatgcctgcg acccggcgca
     7321 ttccaccggc cacatgccgc ggtcctgggc gaactgccag ctggtatcga acggatacat
     7381 atctgccacg acaatatccg cctcagacac taccggcacg gcatacatcc gctgtgccgc
     7441 acgaacccct tcctcatggg ccttgaccac atcaccggca aacaagccgc taatttcgcg
     7501 acggtggttg agcgtgacat tggcaataaa atcgaggccg gccttcctgg ccactgccac
     7561 catatcctgc cgcagctctg tatggatcga accgcctcgt gtgcgtgaac cacgcaagta
     7621 gtcgtgcatc atcctcgtgg tctccacacc gcagacggca gggagcacaa tttttgcgcc
     7681 gcccgagaac ccggcgatcg gatgaggata gatgcacccc actccgatct tcagatcaca
     7741 ttccatgacg gtgcggttga tccgaatcgg caacccgccg ggcgacatgc ctaaatccac
     7801 gagggctccc cggctgtcgt gcgccatgac tcgcacggcc ggcgagaggt tggcgccgac
     7861 ctttttctct atttcctgcg cggaggcggg aggatgagtg ccacctgcca agagaatggt
     7921 gatcgcgtgc tcacttaatc cagctgcttg gatctctgcg ataatcgacg ggaggaggtc
     7981 tgccgcagga gtcggccgcg tgagatcgtc gacgatgatc acgcatttgg tctttccctt
     8041 ggcgatctca cggagtggtg gagaaccgat gggtacattc aatttttcgc gcatggccgt
     8101 ggcactcaat gccggggcgt cctgagggcc gacctccaca atattccacg caggcggaaa
     8161 attgatcgat agcggctcat caccgaacca cgccttcgtg cgtaactgca cgtcgctcat
     8221 gatttgctct ccaccctgga agaaaccgct tgcaccatag gtttcgtctt acggtcaatg
     8281 tgagtcatgg accttttccg gcacatctcc cagaaaaacc cggcggcctg acatttttct
     8341 cccagccaaa agagacaaaa atgtcgttta cgctgaaact tctgccattc gaacggcccc
     8401 aacattttta cccagatggc cttcagccaa cgcggcttgg ttttctttcc accctcgccc
     8461 cacaacagac tgtcctgcgg caccactccg ctggaatccc tacgcatgac ccggaataaa
     8521 atcgcaattc ccgcgcctcg atgaaaggaa tgtcgcagat gttgcagtcc tgtcgcccgc
     8581 cgcagatggg tcacgggatt gggaacaaac accatcttgg cgccccgctt gcgcagccgc
     8641 caaatcaatt cccaatcctc gcctgcgccg gtgaagagcg gattgaagac atacccatct
     8701 tctcgctcgc atttcaggag gcaatcacgc cggaagagaa tgtttcccgc gttacctacc
     8761 aatcctcccg gatcgagctg ttcaaccgtc ggaagcgaat tgagacgacg aatggcatcc
     8821 aggagcggat cggtttcttt gaacgtgcca ccggcgacgg cagcatcgta gtgatcgatg
     8881 gcggttacca accgttccag ccagtcttgt ggcggaacgg cgtcgtcgtc gacgaacgcg
     8941 atcaggtcgc ccgtagaccg ggcaatgccc tcgtttcgtc cccacgcgac cccgcggcga
     9001 tcgcggtcga gaacaacgat gacttcatcc ggcaaccgag tctgtttcga aagcccctcc
     9061 tggcacaacg cgagcgtctc ccgcccgaga gtaggaatca cgacagagat cttgtgctga
     9121 tcagtatgga gtttcagcgt tccccctcct agttggatgc cgcagctttc aaccgcaata
     9181 acaacggagc caccattcga agcacagact ctcctgcctt ccgaaacgtg cccggcagac
     9241 tctgcacgag cgattccagc tcgtccacct gcacgatgcc aaggctgaag acgccccccc
     9301 aatacagaaa gactccaacg gcaatgccca ccagaagaga cagcatcggc gtagcgacga
     9361 gtgcggagag cccggccatt gcgataccgg tcatgacgac aagccccact aatcgcgaca
     9421 gcgctagccc ctcgaagacg ctgacgttcc gccgccgtaa tatccagaga tagccgcccg
     9481 ccaccagcgc aaaggagagg gacgtcgcac aagctgcgcc ggctgcacca ccggcgggaa
     9541 tcaggatcgc gcagagtccg gcatccacca tgaccgtcag gagcatgacc cggatcaggg
     9601 acattgcctc accgccggct tgaatgaccg gccacagcac tcgggccaag gcataacaca
     9661 acgcgccggg caccagcaga cgcaacccca gggcagcctc tgaaaattgg gagccaaagt
     9721 acaccgtgat aatccggtcg cccaacacaa acaccacgat cagcagaaag accgtgccca
     9781 gcatgacgta gcgtacgaga cgactcacca ggctggtcac agcctctacc cgctgttcag
     9841 cccacagccg agcggtcgct tgcagcatca ctccttcgac cgcaatggga atgaaccaga
     9901 cgaattggga ccactgcacc gccgccgcgt agaggcccgc ctgagcttcc ccagccaaat
     9961 gtcggacgag aatgatgtcc agtgagcaga gcgccatgtt gagcacggaa aaaatcatgg
    10021 ccgacaatcc gaatcgaagc acggctcccg tcggcaaagc gggaggtccc tgagacgccg
    10081 gaagcccgag gccgttcaat gccaccatca ccgctctcag acaggcgcct gccacaaaga
    10141 tatccgccac cagcagaccg accaagacgc caaccacgcc catcccggtg accgccgcga
    10201 taatgccgaa acttcctgcc atcaggacgc ccatgatgcc gggcaccgcc gcgcgttctt
    10261 catgtcgtaa accgtgcaac acgccgcgcg cataaaacca gacctggtca aatacgagga
    10321 cgcccaccac ggccaacgca atcggcacgc gcgctgtctc gtgcgacgcc gtgccccaga
    10381 tcaacactcc ggccgcaagc ccgacaaaca cgactcccgt gccattgatg agcgcgccgg
    10441 agcgcgcgac cgcccggcac catgccgcat cgtcaggccg ctcggcgatg cgcctggtca
    10501 tcgtttgcat catgcccaca ttggcaaatt gcgaggcgag caagagcacc gccaggtaat
    10561 aagcgtactg accatatcct tcgatgccga gaaatcgcgc gagaataggc aggacgacgg
    10621 ctgacgtggc cagcttggcc atcgaccacc cgccgacgga gagaattcct ttagaaaccg
    10681 ctttcgccga catggctctt caacatcgaa cactccgctg ttccgaagtc cgtatacacg
    10741 cccttcacag acgcttatgc cgcaaaacgc gcatattgct cttgatgggc aatcaacgct
    10801 ctggaggttt cgtaggtgtc ccgcacatag tgataggccg cccggccctt ctccagcggc
    10861 gcctcggcga gcaggctctg cagcgcggcg gcatagtcgt catcgtgcac ctgctttcca
    10921 aaccgggtcg cgaactggtc gggatccacg cggctgatga tgggacaccc gtaggccgcc
    10981 gcttccagga acgttaatgg aaggccttcg cgcaccgccg tgctgacgaa gacccaggtg
    11041 tccgacaaaa tttggtgcat tcgctccggc tcatgaaatc gattgatcag gcccggcatt
    11101 tcaaggttgg gcacgttgcg gaatcgctgt cgcaactcgg cgtcaaatcc ggtttcggcg
    11161 gaagcgctcc ctttgcccac ggccacgaac cgatagtctg ggaactgcgc agccagttcc
    11221 aaaaacagcc aggggcgctt gcgtttgtcc caacgggcaa taaaggtgat cgtcggccgc
    11281 tcgctttttc gagggagcgc cgagggcacg tcaatgagat tggggagcag cgtcggcaac
    11341 tgcttgagcc catacaggcg cttg
//
LOCUS       DAOQEW010000123        11271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1745_length_11271_cov_7.370275, whole genome shotgun sequence.
ACCESSION   DAOQEW010000123 DAOQEW010000000
VERSION     DAOQEW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_11271_cov_7.370275"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..360)
                     /locus_tag="PLS35_13045"
     CDS             complement(13..360)
                     /locus_tag="PLS35_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19642.1"
                     /translation="MRCPGMTLSCLLLFAGCLVSPASADTSRERVALFLSGQDCPSSR
                     RNIAAELAQVAGVIRVDMESVPDHALVDVVIGAVSPDDLLVAVHRSALKGVDCRAEVM
                     KSCISAGRSAADR"
     gene            complement(473..1438)
                     /locus_tag="PLS35_13050"
     CDS             complement(473..1438)
                     /locus_tag="PLS35_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNI19643.1"
                     /translation="MYTHTDHRGGRTGKTIMFIGVMLSMLAGMALSAQAKTHDIKMTA
                     VETDIVIDGGGEKYAAWTFNGQFPGPVVRVTEGDTINFTLTNPATNKNPHAMDFHAAE
                     IDFLKNYRAVNTGESISFTFVAKKPGLFFYHCGAPPMIQHVARGMFGAIIVDPKNAKV
                     WPKADREYVLVQSEYYKNPNDVQAMFDRKYDGMMFNGGIFKYHPFVTGGGKLDAKPGE
                     RVRIYFVNAGPNEFSSFHPIGEIWDNVYESGNPANNLKGVQTYVVGPGSAATFDVVVE
                     SAGAYPLVTHSLTGALRGAIAVLLAGPDAKPAPLMPMVPWEIPAK"
     gene            complement(1611..2351)
                     /locus_tag="PLS35_13055"
     CDS             complement(1611..2351)
                     /locus_tag="PLS35_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNI19644.1"
                     /translation="MSKQPKRSPQQPAAERPSPGAPAGLNQIPLLQILSAQDRQKVLG
                     EMTETRYGKGDYIFREGDPTEYFHIVKEGSVKCVKSSLDGKECTLKVLMPGDLFCCDA
                     SAFDGASHPGTAQPMGDVSVLRMKKEAYFKMLRANPDAAMEVIRYLGNRLNEAQEKAK
                     VLALDRADQRLASLLVSLAERSGIKDPNGIRVTVRLTRQDMANMVGVTTETAIRIMAR
                     FKKDRLVTGTAARLVIRDLPKLKLLAST"
     gene            complement(2308..4194)
                     /locus_tag="PLS35_13060"
     CDS             complement(2308..4194)
                     /locus_tag="PLS35_13060"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNI19645.1"
                     /translation="MTSPSGSTTTPDLLTALANTATNLRIDSVKATSEAGSGHPSSCC
                     SAADILAVLFFSVMRYDPKNPKLPNSDRFVLSKGHAAPLLYAAWAEAGLFPKSELLKL
                     RTLGSDLEGHPTPRLSFVDMATGSLGQGLPAGVGLAFNAKSIDKTDYRTYVLMGDGES
                     VEGSVWEAAEVARQAKLDNLCAIVDVNRLGQSDPTMLQHDMDAYRARWSGFGWHAIVV
                     DGHDISALVAAFAEAAKTKGQPTVLLAKTFKGRGISFMENHPDWHGKPLKKGEETQKA
                     LDELTGQLKPSSTRLQISLPTAAKTAPSTKGTLAPAPYKVGDSVATREAFGAALLALG
                     EANSQVVALDADVKNSTYSDKFGKRFPDRFLENFIAEQNMLGAAAGIAACGKIPFVAT
                     FAAFFTRAYDFIRMAAISQSNIKLVGTHVGVSIGEDGPSQMGLEDLAMMSTQPGVTVL
                     YPSDGLSMYKLMESATAHKGMVYLRAGRPKTPVIYGADETFKIGGSKILRQSAADQLT
                     IVAAGVTLFEALKAHDQLKAAGIATRVVDLYSIVPVDQATLIDCARATGGRFLTVEDH
                     YAHGGIGDAVLSALASEGVRVRKLAVRDIPRSGKPDELVDHFGIGVRSIVEAAKEIAT
                     AARR"
     gene            4503..5087
                     /gene="pyrR"
                     /locus_tag="PLS35_13065"
     CDS             4503..5087
                     /gene="pyrR"
                     /locus_tag="PLS35_13065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNI19646.1"
                     /translation="MPPADRPLMTTESTTGNRQERVVMDAGDIARAVTRIAHEILERN
                     KGIQDLALVGIRTGGVHLAHRLVRRIHDIEGTQIPIGELDITLYRDDLSLRKDQPILR
                     TTSVPFKISDLKVVLVDDVLFTGRTIRAAMDGLMDLGRPAEIQLAVLVDRGHRQLPIK
                     ANYIGKNIPTSRDEAIEVHLEENGEEDRVVILRT"
     gene            5102..6025
                     /locus_tag="PLS35_13070"
     CDS             5102..6025
                     /locus_tag="PLS35_13070"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNI19647.1"
                     /translation="MGLKRKDLLSLTNLSAADIALILDTADSFKEVSGREIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSSSSVVKGETLLDTARNIEAMQA
                     DIIVLRHSSAGAAETLARGVKSSVINAGDGWHEHPTQALLDLYTIRSRGMGFSGLKVA
                     IVGDVAHSRVARSNIFALTKLGAEVRVVGPPTMIPLHINQLGAQVYYNLDEGLRGVHV
                     IMMLRLQLERQGRALFPTIREYARQYGLTNERVKLAEPGAIVMHPGPINRGVEIAPDV
                     ADSLSSVILDQVANGVAVRMGILYLMSGANA"
     gene            6115..7404
                     /locus_tag="PLS35_13075"
     CDS             6115..7404
                     /locus_tag="PLS35_13075"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNI19648.1"
                     /translation="MILIKGGHVIDPGRVNGIADVLIENGKIAAVGQKLAAPAGATVI
                     QAGGQLVLPGFVDLHVHFREPGFEYKETIQSGTDAAVAGGFTTVCAMPNTNPVNDNQA
                     VTEFMLERAKAAGTANLYPIGAITKRSEGKELAEIGDLRRAGCVAISDDGKPVMNSLV
                     MRRAMEYARAFDVPVVDHCEDLHLSEGGCMNEGLISTELGLPGIPSAAEDVMVARNVS
                     LAELTGARLHLAHISTAGSVRMVREAKARGLKVTAEACPHHFTLTEETTRGYNTHAKM
                     NPPLRTSQDVQAIKDGLRDGTIDVIATDHAPHASQEKQQEFTEAPFGIVGLETALPLT
                     LTLVEEGVLTLESAVDKLATAPAKAFSLNAGTLAVGAPADVAIVDPNLEWEVDPSRFR
                     SKSRNTPFAGWKVKGRVTTTLVSGRVVYELDRPGRQA"
     gene            7417..7926
                     /locus_tag="PLS35_13080"
     CDS             7417..7926
                     /locus_tag="PLS35_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNI19649.1"
                     /translation="MRQGLIACVTFEMLALAVWIGGLLVLVAAVIPAVFNTFGGQDTG
                     GFFLTRAFDGYNRLVLGAAAILIAGILWRAWLYQQGMTDEAITRAEWLLLGAMLLIAG
                     VITFVLHPQAAALQAQAFASKGEDARKAAFEAFFQLHKPVRVLYILNVGLGIALLTLR
                     VRSWIRVKE"
     gene            7929..9113
                     /gene="carA"
                     /locus_tag="PLS35_13085"
     CDS             7929..9113
                     /gene="carA"
                     /locus_tag="PLS35_13085"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNI19650.1"
                     /translation="MKKAILALADGTWFEGRALGAEGETGGEVVFNTAMTGYQEVLTD
                     PSYRGQIVTMTSPQIGNYGVTPEDVESTKIWAEGFIVKESSRLASNWRGKSTLQEYLQ
                     AAHIVAIEGIDTRALTRHLRERGAQPGVISHIDLDPRRVAEKARQAPSIIGRDLAATV
                     TCEHRYPWTEGTGDWAPKITMPEQGAAKAPRKTWRVVAYDFGVKQNILRRLVDVGCEV
                     TVVPASTPAKDVLALNPQGLFLSNGPGDPEGVPYAMTALRELIGRVPIFGICLGHQLL
                     GLALGFSTYKLKFGHHGANHPVIDLRTRKVEITSQNHNFAVRFPGETPVVGQELPIVD
                     TPFGRVQLTHTSLNDASVEGMRCLDRPVFSVQYHPEASPGPHDSAYLFEQFVALMETH
                     HA"
     gene            9271..10260
                     /gene="sppA"
                     /locus_tag="PLS35_13090"
     CDS             9271..10260
                     /gene="sppA"
                     /locus_tag="PLS35_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNI19651.1"
                     /translation="MKRNVGQAPDVWQLSTGLLVLALALMQAACVTINLPPGPGALEE
                     HKVSGTGKDKVLLMDVSGVISSENKDGFYSSPGMLATVKEELERAAKDERVKAIVLRI
                     NSPGGTVTASDIIYHELRNFKTSRKIPIVASIMDVGASGGYYIAAAADTVLAHPSSVT
                     GSIGVIMLTVNARGLLEKVGVETNAVTSGPRKDMGSPFRAMLPEERAIFQGVIDGFYQ
                     RFLQVVQEGRPGMNGETIKKLADGRIYSGEQAKAAGLVDDIGYLDDAIDLAKKKAGLT
                     DAKVVTYRRPGEYQNNVYSRLVAPAPSLASLANVDLLSVVRGGSPQFMYLWMP"
     gene            10311..>11271
                     /locus_tag="PLS35_13095"
     CDS             10311..>11271
                     /locus_tag="PLS35_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNI19652.1"
                     /translation="MYPSNRHSIETLLKTPIGRRTALALGARAASLLATTMGLGAATG
                     LLQSLGGCQRAPGTARDQFIYLSEEKEMAMGLSAFREVLRQAPLSDNPELNEMVHRVG
                     NRIAKAANKPEYQWEFAVIQDDRTINAFALPGGKVAVFTGILKVTKTEDGLATVIGHE
                     VAHALQRHGAERMSRGILEQIGQLAALGAGAAVGRPDAAMAAMTVYGVGVSLPFDRRQ
                     ESEADFIGLRLMAEAGYDPREAVSFWERMSGCPRAMINKLCFRSQQAIPEFLSTHPSD
                     VTRINQIEAWIPDAMKHYHPAGKAPAIPSGPIQPYRPPVGPMPE"
BASE COUNT         2294 a         3394 c         3383 g         2200 t
ORIGIN      
        1 ggcacgtgct ggtcaccgat cagctgcaga gcgaccggcg gaaatgcatg acttcatgac
       61 ctccgcccga caatccacgc ccttgagggc ggagcggtgc accgccacca gaagatcgtc
      121 cggactcacc gcaccgatca cgacatcgac cagcgcatgg tcaggaacgc tctccatgtc
      181 cacgcggatg acgcctgcta cttgcgccag ttccgcagcg atattccggc gggaagaggg
      241 acaatcttgg ccggataaga agagcgccac tcgctcgcgc gaggtgtcgg cactggctgg
      301 actcacgagg cacccggcga acaggagtaa gcaactcagc gtcatcccag gacatcgcat
      361 cggcatctcc aagaaaccac gatccatgca taaacggtgt cgtcattgtt gatctctcgc
      421 gttgtccggc cgacccctta ggggccggcc gaagattcga tcacgaaacg gtctacttcg
      481 ctgggatctc ccacgggacc atcggcatca acggcgccgg tttcgcatcc ggtcccgcca
      541 gcaacaccgc aatcgcccca cgcaatgccc cggttaagga atgcgtgacg agcggatagg
      601 ctccggcaga ttccaccacg acatcaaacg tggcggcact gccgggccct acgacgtacg
      661 tctgcacgcc cttcagattg ttggcaggat tgccactctc gtacacgttg tcccagatct
      721 caccgatcgg gtggaacgag gagaactcgt tcggccccgc gttgacgaaa tagatgcgga
      781 cgcgctctcc cggtttggca tcgagttttc ccccgccggt cacgaagggg tgatacttga
      841 agatgccgcc gttgaacatc atgccgtcgt actttcgatc gaacatggcc tggacatcgt
      901 tcgggttctt ataatactcc gactgcacca gcacatattc ccgatccgct ttcggccaga
      961 ctttagcatt cttgggatcg acgatgatcg cgccgaacat gccgcgcgcg acatgttgaa
     1021 tcataggcgg cgctccgcaa tggtagaaaa acagtcccgg ctttttcgcc acaaaggtga
     1081 agctgatgct ttcgccggta ttgacggccc gataattttt caggaaatcg atttcagccg
     1141 cgtggaagtc catcgcatgc ggattcttgt tcgtcgcagg attcgtcagg gtgaaattga
     1201 tcgtgtcacc ttccgtcacc cgcacgaccg gccccggaaa ctgcccgttg aacgtccagg
     1261 cggcatattt ttcaccgccg ccgtcgatca caatgtccgt ctccaccgcg gtcatcttaa
     1321 tgtcatgcgt tttggcctgg gccgacagcg ccatgcctgc cagcatactc agcatgaccc
     1381 cgatgaacat gatcgttttg ccggttctcc cccctcgatg gtctgtatgt gtgtacatgt
     1441 tgcctccgcc attcctgtgt tgaacccggc gaccggaagc gcaatgctcc cggatcaaca
     1501 ttcgtcgcga tcgaatgctg cgcctctggt tgcaccatac gagatggcgg catcaacaaa
     1561 acatgactat tgtcatgttt ttaaaaatac ccggtctgtg gcgaaagaaa ttacgtggaa
     1621 gcgaggagtt tgagcttggg gagatcacga atgacgagcc gcgcggcggt gccggtcacc
     1681 aatcgatcct ttttgaagcg cgccatgatc cggatggcgg tttccgtcgt gacgcccacc
     1741 atgttggcca tgtcctggcg cgtcagacga acggtgaccc gaatcccgtt ggggtccttg
     1801 atcccgctgc gttctgccag actcacgagc aaagaagcca atcgttgatc ggctcgatcg
     1861 agggcaagca ctttggcttt ttcctgggct tcattgagcc ggtttcccaa ataccggatg
     1921 acttccatcg cggcatccgg attcgcccgg agcatcttga agtaggcttc ctttttcatg
     1981 cgaagcacac tgacatcgcc catgggctgg gccgtgccag ggtgggacgc accgtcgaag
     2041 gccgaggcat cgcaacaaaa caaatcaccc ggcatcaaga ctttgagcgt gcattccttc
     2101 ccgtcgagcg acgacttgac gcatttgacc gacccttcct tgacgatgtg gaagtattcg
     2161 gtcggatcgc cttcgcggaa aatgtagtcg cccttcccgt agcgggtttc agtcatttcg
     2221 cccaggacct tttggcgatc ctgggcgctg aggatctgga gcaggggaat ctgattcaac
     2281 ccggcaggag caccggggct gggccgttca gcggcgggct gctgtggcga tctctttggc
     2341 tgcttcgaca atcgagcgca ctccaattcc aaaatgatcc acgagctcgt ccggttttcc
     2401 gctacgtgga atgtcccgga cggccaactt gcgcacccgt acgccttctg atgccagggc
     2461 gctcaacaca gcgtcgccga tcccgccgtg cgcataatga tcttcgaccg tcagaaaccg
     2521 tccacccgtc gctcgcgcac agtcgatcaa ggtggcctgg tcaacgggca cgatgctgta
     2581 taaatctacc acgcgggtgg cgatgccggc tgccttcagc tggtcgtggg ccttgagggc
     2641 ttcgaacagc gtgacaccgg cggcgacgat cgtgagctgg tcggcggcac tctgtcgcag
     2701 aatctttgaa ccgccgatct tgaacgtctc gtccgcacca tagatcacgg gggtcttggg
     2761 acgaccggcc cgtagataca ccatcccctt gtgggcggtc gccgattcca tcaatttgta
     2821 catcgacagg ccatcggacg ggtagagcac ggtcacgccg ggctgggtcg acatcatggc
     2881 gagatcttca agtcccatct gcgacgggcc gtcctcaccg atgctgacac cgacgtgagt
     2941 ccccaccagt ttaatgttcg actggctgat cgccgccatg cgaataaaat cataggcgcg
     3001 cgtgaaaaac gccgcgaagg tcgccacgaa cggaatcttg ccgcaggccg caatgccggc
     3061 cgcagcgcca agcatattct gctcggcgat aaagttttcg aggaaccggt ctggaaaccg
     3121 tttaccgaac ttatcactgt acgtagagtt cttcacgtct gcatcgagcg cgaccacctg
     3181 agagttcgcc tcgccaagcg ccaggagggc ggccccgaag gcttcccgcg tcgccaccga
     3241 atcgcccacc ttgtacggtg ccggagccag cgtgcccttc gtggacggag ccgtcttggc
     3301 tgcagtcggc aacgagatct gaaggcgggt cgagctcggt ttcaattggc ccgtcaattc
     3361 gtccaacgcc ttttgggttt cttcgccctt cttcagcggc ttcccgtgcc agtccggatg
     3421 attttccata aaggaaatgc cgcgcccctt gaaggtcttt gccagcaaga ccgtcggctg
     3481 ccccttcgtc ttcgccgcct cggcgaatgc cgcgaccagg gcgctgatat cgtgaccgtc
     3541 caccacgatc gcatgccacc cgaacccgct ccaccgggcg cggtaggcgt ccatatcatg
     3601 ctgcagcatc gtcggatcgc tttgtcccaa ccgattcacg tccacgatgg cgcacagatt
     3661 atccagcttc gcttggcgag ccacttccgc ggcttcccac acagacccct cgacagattc
     3721 gccgtcgccc atcaggacat aggtgcggta gtcggtcttg tcgatggact tcgcattgaa
     3781 cgcgagcccg acacccgccg gcagcccctg gcccaatgaa ccggtcgcca tgtcgacgaa
     3841 cgagagtcgc ggcgtcggat ggccttcaag atcggagccg agcgtgcgca atttcaacag
     3901 ctccgacttg ggaaataatc cggcttccgc ccaggccgca tagagcagag gagcggcgtg
     3961 ccccttggag aggacaaacc ggtcgctatt cggcaacttc ggattcttgg ggtcgtaccg
     4021 catgaccgag aagaacaaca ccgcaagaat gtccgcggcc gagcaacagc tggacggatg
     4081 tccgctccct gcttcgctcg ttgctttgac gctgtcgatg cggaggttgg tcgctgtatt
     4141 ggcgagggcg gtcaacagat caggagttgt ggtggaacca gaaggggaag tcatgcgcga
     4201 tcctttcacg atgggagctg atgattctgg atgacctgaa tgacaaagag atcgagagcg
     4261 agagtccgct ctcggtggga cgaagctaac aaatggcatg catggagtca atgaaggcat
     4321 gtccctctca tcagcccatc gcctgcagtg aattcttgac acggttggat caactcgtta
     4381 agatcggctc gcctttaacg ctcatcctgt gaggtgggta aggagctcat gatcatgatg
     4441 cacggctcaa gccgtccgac aacccgcacc ttctcgtcca caccggagga gaaggttttt
     4501 ttatgcctcc agctgatcgg ccactcatga ccacggaatc tacaaccggc aaccggcagg
     4561 agcgcgtcgt gatggacgcg ggcgatattg cccgcgccgt gacccgcatt gcccacgaaa
     4621 tcctggaacg gaataaaggc attcaagatc tcgccctcgt cggcatcaga acgggcggcg
     4681 tccacctggc gcatcgactc gtgcggcgca tccatgacat tgaaggcacc cagatcccga
     4741 tcggcgaact ggatatcacg ctgtaccggg acgacctctc gttgcggaag gatcaaccga
     4801 tcctccgcac cacctcggtg ccgttcaaaa tatctgacct gaaggtcgtg ctcgtcgacg
     4861 atgtgctctt cacaggccgg accatccgtg ccgccatgga cggactgatg gatctgggac
     4921 ggccggcgga aatccagctg gcggtgctgg tcgatcgcgg tcaccggcaa ctcccgatca
     4981 aagccaacta tatcgggaaa aacattccca cctcgcgcga cgaggccatt gaggtccatc
     5041 tcgaagaaaa cggggaagag gaccgcgtgg tcatcctcag gacgtaaccg gagacagtga
     5101 cgtggggctc aaacgcaaag accttctcag cctgaccaat ttgtcagctg ccgacattgc
     5161 cctgatcctg gacacggcag attcgttcaa agaagtctcc gggcgggaga tcaagaaggt
     5221 gccggccttg cggggcaaga cggtcgtgaa cctctttttc gagccgagca cgagaaccag
     5281 aacctcgttt gaattggccg ccaagcggct cagcgccgat gtcatcaatt tttccccttc
     5341 gtccagcagt gtggtgaagg gcgaaacgct gctggatacc gcgcgcaata tcgaagccat
     5401 gcaggcggat atcatcgtgt tgcggcattc atcggcaggc gcggcggaaa cactggcgcg
     5461 aggcgtcaag tcttccgtca tcaatgccgg cgacgggtgg cacgaacatc cgactcaggc
     5521 gctgctcgat ctctacacca tccgcagccg cggcatgggg ttctctgggc tcaaggtggc
     5581 gattgtcggc gacgtcgcac acagccgcgt ggcccgttcc aatattttcg ccctcacgaa
     5641 actcggagcc gaagtgcggg tcgtcggacc accgacgatg attccgctac acatcaacca
     5701 gctcggcgcc caggtgtatt acaacctgga tgaagggttg cggggcgtgc atgtgatcat
     5761 gatgctgcgg cttcaactcg aacgccaggg gcgcgccttg ttcccgacca ttcgcgaata
     5821 tgcccggcag tacggcttaa ccaacgagcg ggtcaaactg gctgagccgg gcgcgatcgt
     5881 catgcatccg ggccccatca atcggggcgt cgaaatcgca ccggacgtag cagacagcct
     5941 gtcgtccgtg atcctcgatc aagtggccaa cggcgtggcg gtgcgtatgg gcattttgta
     6001 tctcatgtcg ggagccaatg cgtaagacgt gatgagacga tctcctcaca ggagatcgcc
     6061 ggcacatgaa accatggttt acattgttta atagggggag ttgaacagcg cactatgatt
     6121 ctgatcaagg gcggtcacgt tatcgatccc ggacgcgtca acggcatcgc ggatgtcctc
     6181 attgaaaacg ggaaaattgc agcggttggg caaaagctcg ccgcaccggc cggcgcgacg
     6241 gtgattcagg ctggggggca gttggtgctc ccgggcttcg tggatctcca cgtgcacttt
     6301 cgcgaaccgg gctttgaata caaggaaacg attcagtcag gaacggacgc ggccgttgcg
     6361 ggaggtttca ccacagtctg cgcaatgccc aacaccaatc ccgtgaacga caaccaagcg
     6421 gtcaccgaat tcatgctgga gcgggcgaaa gcggcaggca ccgccaacct ctatcccatc
     6481 ggcgccatta ccaaacgctc cgaaggcaag gaattggcgg agatcggcga cctgcgtcgc
     6541 gccggatgtg tcgcgatttc cgacgacgga aagccggtga tgaacagcct cgtcatgcgc
     6601 cgcgcgatgg aatatgcgcg cgccttcgac gtaccggtcg tcgaccactg cgaggatttg
     6661 catctctccg aaggcggctg catgaatgaa gggctgatct cgaccgaact gggactcccg
     6721 ggcattccgt cggccgcgga agatgtgatg gtggcgcgca atgtctcgct cgccgaactg
     6781 accggagcgc gtctccatct ggcgcacatc agcacggccg gttcggtccg gatggtgcgc
     6841 gaagccaagg cgcgggggct caaagtgacg gcagaagcct gcccccatca ctttaccctc
     6901 acggaggaaa cgacccgcgg ctacaacacc catgcgaaaa tgaatccgcc actgaggacc
     6961 agtcaggatg tgcaggcgat caaggacgga ttacgtgatg gcaccatcga cgtcattgcg
     7021 accgaccatg cgccgcacgc cagccaggag aaacaacagg agttcaccga ggcgccgttc
     7081 ggcatcgtag gactggaaac cgcgttgccg ctgacgctga ccctggtcga agaaggggtg
     7141 ctcacgctcg aatcggccgt cgataagctg gccacggctc cggcgaaggc gttcagtctc
     7201 aatgccggca ccctggcggt cggcgcgccg gcggatgtgg ccatcgtcga tcccaatctg
     7261 gaatgggaag tcgatccctc gcgatttcgt tccaagagcc ggaacacgcc gttcgccgga
     7321 tggaaagtca aagggcgtgt caccacgaca ctcgtctccg ggcgggtcgt ctacgaactc
     7381 gatcgcccag gacggcaggc gtaggacgag acgatcgtgc gtcaaggatt gatcgcctgt
     7441 gtgaccttcg agatgctggc attagccgtc tggatcggcg gacttctcgt cctcgtggca
     7501 gctgtcatcc ccgccgtgtt caatacgttc ggcggacagg acaccggggg atttttcctg
     7561 acgcgcgcgt tcgacgggta taatcggctg gtgcttggcg ccgcggccat tctgatcgca
     7621 ggcattctct ggcgagcctg gttgtatcaa cagggaatga cggacgaagc catcaccaga
     7681 gccgagtggt tgttgctggg cgcaatgctg ctcattgccg gtgtgatcac gtttgtgctg
     7741 catcctcagg cggccgcctt gcaggcgcag gcctttgcct ccaaagggga ggacgcgagg
     7801 aaggccgcct ttgaggcgtt ctttcagcta cataaaccgg tacgggtgct gtatatcctg
     7861 aatgtgggac tcggtatcgc gctgttgacc ctgcgcgtgc ggtcctggat ccgagtgaag
     7921 gagtgagagt gaagaaagcg attcttgcgc ttgccgatgg aacctggttc gaagggcgag
     7981 ctttaggcgc ggaaggggag accggcggcg aagtcgtctt caacacggct atgaccggct
     8041 atcaggaagt tctgaccgat ccttcgtatc gcgggcagat cgtcaccatg acgtctccac
     8101 agatcggcaa ctacggcgtc acgccggagg acgtcgagtc gacgaaaatc tgggccgaag
     8161 ggttcatcgt gaaggaatcc agccggctgg cgagtaactg gcgaggcaag tcgacgctgc
     8221 aagagtatct gcaggccgct catatcgtgg cgatcgaggg catcgatacc agagcgctca
     8281 ccaggcattt gcgggagcgg ggcgcccaac cgggcgtcat ttcccacatc gatctcgacc
     8341 cccgccgcgt ggccgagaag gcccggcagg cgccgagcat catcgggcgt gatctggcag
     8401 ccaccgtcac ctgcgaacat cgttacccct ggacggaagg cacgggtgat tgggcgccga
     8461 agatcacgat gccggaacag ggggccgcga aggcaccgcg caaaacctgg cgcgtcgttg
     8521 cctatgattt cggtgtgaaa cagaacattc tgcggcggct ggtggatgtg gggtgcgagg
     8581 tcaccgtggt gccggcctcg actccggcaa aggatgtgtt ggccctgaat ccgcaaggat
     8641 tgttcctctc gaatggtccc ggtgacccgg aaggtgtgcc ctacgccatg accgcactcc
     8701 gtgagctcat cggccgcgtg ccgattttcg gcatctgcct ggggcatcaa ttgctggggc
     8761 tcgcgttggg attctccacg tacaaactga agttcggcca ccatggcgcc aatcatccgg
     8821 tcatcgacct gcgcaccaga aaggtcgaaa tcacctctca gaatcacaac tttgccgtgc
     8881 ggttccccgg cgaaactccg gttgtgggtc aggaactgcc gatcgtggat acgccgttcg
     8941 gtcgggtgca attgacccac accagcttga atgacgcatc cgtggaaggc atgcggtgtc
     9001 tggatcgccc ggtgttttcg gtgcaatacc atcccgaggc gtctcccggt cctcatgatt
     9061 ccgcctatct gtttgaacaa tttgtcgcat tgatggagac acaccatgcc taggagcctg
     9121 tccgatattg accgctctgc tgcggcgaat gacctgcggc catctgcgtc gttctcggcc
     9181 ctcaaaaatc ctcaatgtaa ttctgcgaat acacttccgg ttttttcagg cctccggcct
     9241 cgcatctggc cgcagctcct tcgcctcgct atgaagcgca acgtcggtca ggctcccgac
     9301 gtatggcagc tctcgaccgg tctgcttgtg ttggccctgg ccctgatgca ggcggcctgc
     9361 gtcacgatca acctgccgcc gggccccggc gcgctcgaag aacacaaggt cagcgggacc
     9421 ggcaaagaca aggtcctgct catggatgtg tcgggcgtga tcagctccga aaacaaggac
     9481 ggcttttatt cttcgcctgg catgctggcc acggtgaagg aagagctgga gcgggccgcc
     9541 aaagacgaac gggtcaaagc gatcgtgctg cgcatcaaca gcccgggagg gactgtcacc
     9601 gcgtccgaca tcatttatca cgaactcaga aactttaaga ccagccgcaa aattcctatt
     9661 gtggcctcca tcatggacgt gggcgcgtca ggcggatact acatcgccgc cgcagccgat
     9721 acggtgctgg cgcatccctc atcggtcacc ggcagtatcg gcgtgatcat gctgaccgtc
     9781 aatgccagag gcctgctcga aaaagtcggc gtcgaaacga atgccgtcac ctccggtccc
     9841 cgcaaggaca tgggctcgcc gtttcgcgcg atgctgccgg aagaacgggc gattttccaa
     9901 ggcgtgatcg acggctttta tcagcggttt ctgcaggtgg tccaggaagg ccgcccgggc
     9961 atgaacgggg agaccattaa gaagctcgcc gacggccgga tctattccgg cgaacaggcc
    10021 aaggctgcgg gactggttga tgatatcggc tacctcgacg acgcgattga cctggccaag
    10081 aaaaaagccg gcttgacgga cgcgaaggtc gtgacctatc gacggcccgg cgaataccag
    10141 aacaatgtct attcgcggct cgtcgcgccg gcgcccagcc tggccagtct ggcgaacgtc
    10201 gatctgctgt cggtcgtgcg aggcgggtca ccgcaattca tgtatctgtg gatgccgtaa
    10261 gccggtgagt tgcttccgcc gcgcggggct gattgaatgg agcagacgtg atgtatccgt
    10321 caaaccgaca ttcaatcgaa acgctgctga agacgccgat cgggcgacgt accgccctcg
    10381 cactgggtgc gcgcgccgct tcgctgttgg ccacgaccat ggggcttgga gccgcgacgg
    10441 gactgctgca atcgctcgga gggtgtcaac gggcaccggg gacggcgcgc gaccagttca
    10501 tttatctgtc ggaagaaaaa gaaatggcga tgggtctctc ggccttccgg gaagtgctcc
    10561 ggcaagcgcc cttgagcgac aaccccgagc tcaacgagat ggtgcaccgg gtggggaacc
    10621 gcatcgccaa ggcggcgaac aaaccggaat accaatggga attcgccgtc attcaagatg
    10681 accggacgat caacgccttc gcgctccctg ggggcaaggt tgcagtcttc accggaattt
    10741 tgaaagtgac caagaccgaa gacgggttgg cgaccgtcat tggtcatgag gtggcgcatg
    10801 cgctgcagcg ccacggagcg gaacggatga gtcgcggcat tctcgaacag atcggacaac
    10861 tggccgcctt aggggccggt gccgccgtgg gacggccgga tgcagccatg gctgcgatga
    10921 cggtctatgg cgtcggcgtt tccttgccct tcgaccgccg ccaggaatcg gaggcggatt
    10981 ttatcggtct gcgcctgatg gccgaggcgg gatatgaccc gcgcgaagcg gtgtcgttct
    11041 gggaacggat gagcggctgt ccccgcgcca tgatcaacaa gctgtgcttc cgttcgcagc
    11101 aggcgatccc ggagttcctc tcgacacatc cgtccgacgt cacccgcatc aatcagatcg
    11161 aggcctggat cccggacgcc atgaaacact atcatcccgc aggaaaggcc ccggcaatcc
    11221 cgtccggtcc gatccaaccc taccggcctc cggtggggcc catgcccgag g
//
LOCUS       DAOQEW010000124        11262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1746_length_11262_cov_9.077898, whole genome shotgun sequence.
ACCESSION   DAOQEW010000124 DAOQEW010000000
VERSION     DAOQEW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_11262_cov_9.077898"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLS35_13100"
     CDS             complement(<1..794)
                     /locus_tag="PLS35_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19653.1"
                     /translation="MKMSTLRARAMIVAILVLMGLISYELSGLVQKAEAIPAFARKYD
                     FKCNVCHVPGFPKLNDFGNLFRDRGYQLGSDADSPVYEGIGMGFWPVSIRATVGYQAA
                     QLRTEGSGVTTGGFGFTGLDLLSFGTLHRDIAFGVVYTPGLGSAGFGTGASDGDLESA
                     FVRLMRLERYFGVKSDPGNYLLNLRVGKFELDLPFSEKRSPTLNTPFVMYHYTPGVPY
                     TATIGGTSTSSYLNPNSFLLGDNQPGGELSGIMKTPSTEGYLRYSLT"
     gene            complement(835..1698)
                     /locus_tag="PLS35_13105"
     CDS             complement(835..1698)
                     /locus_tag="PLS35_13105"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNI19654.1"
                     /translation="MSPRRHRGFRLVLLSCLAWLLTAISGYAEYEIIDVSDGGTVKGQ
                     AVWKGSIPKLPPLKVFADLDSCGTHVPSPALRIDPATNGIHEVLVYLERVERGKSAAT
                     AYPLRMGKSASHPTGRACQFERHMIPFVRTAEVALINFEPILHNPHLFSEKQSLFNIA
                     MPTANREIATTLGRARGVGLRLQCDVHVHMNAWAAALEHPYFAVTDEQGKFEIAGIPP
                     GSYTLVAWHPGFNIVKFSASRPVYDEPHVIRQPVEVSSKAQVESRFEFPVRPVEVEWN
                     IAGGGDELPPE"
     gene            complement(1954..2967)
                     /locus_tag="PLS35_13110"
     CDS             complement(1954..2967)
                     /locus_tag="PLS35_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNI19655.1"
                     /translation="MAGRPLVPDHILQIGFGFWSSKTLLSAVELGLFTLLAKRQLDGK
                     ALAARLKLHPRSARDFFDALVALKLLKRNGTRYANTPETALFLDRAKPTYVGGMLEMC
                     NARLYGFWGSLTEGLRTGQPQNEVKTGEDFFGTLYADPQRLEGFLKAMTGLSKGTARS
                     IAAKFPWKRYHSFADIGCAQGGVAVEIALAHQHLNGQGMDLPVVRPVFESYIRSKKLQ
                     KRMTFHEGDFFKDSLPKVDVLIMGHILHDWDLKEKMMLLRKAYAALPPGGALIVHEAL
                     IDDARKTNAFGLLMSLNMLIETPGGFDFTGADCRQWMKKVGFTRTKVEPLAGPDSMVI
                     GFK"
     gene            3238..3981
                     /locus_tag="PLS35_13115"
     CDS             3238..3981
                     /locus_tag="PLS35_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19656.1"
                     /translation="MATYLHVKAALTALVLFSIMTATAGAEQWNGPQTPTGTIWRPGN
                     IALGFDPAGHSGTPPALEIRRPLSGTEEVLLSLRSTFKEATATVLEVDTKRVYMGGAA
                     TRTSLVPDGVFDLAVGRGIAVGVNSVTERLPTDYRLVVGGKVLAEEVRIKLIKDWADY
                     VFHPDYRLAALPEVATYIQSHQHLPDMPSAAEVAATGISLGDMQAKLLRKIEELTLHA
                     IEQHQTIAALQARLQQLEEDRSSQQLGSK"
     gene            4019..4936
                     /locus_tag="PLS35_13120"
     CDS             4019..4936
                     /locus_tag="PLS35_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19657.1"
                     /translation="MTQFTALTTALLLATLTLPAEAGTTSLTLNQVIKEGTARYYDHP
                     QELRLADGFWAQAGSDMTATIHLQQGYYLDKTPLDTKEKQYTSTDGRTYLVAGCVPKH
                     LPISGPVGSVWVACNDLDYLKDKVVESRFVSCLDVFTNGVVSGVSIKEVNNRTWESFR
                     FSCRDMGPDGSMVGAAQKSDFLFNYEREGKLYETTTASGHLTFGILEVANGLTGTKSL
                     LQIALEHTAAAALQAAGVNGRHVDDFTLSDKVPDAFGLGGMLRVDHWKCPPGMVITGM
                     AIGHNPDKKGNDTKPVYILAECRKLLYTP"
     gene            5101..5766
                     /locus_tag="PLS35_13125"
     CDS             5101..5766
                     /locus_tag="PLS35_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19658.1"
                     /translation="MATMLQKALQGLLLVLLVGCSTTYEPHLVLPPAEFNRSIDAAVE
                     VHPFVAADDLLYGRSNYGVVADDYANKPPSKLTQSITQQVTAELAAHRVFRRVSTYDP
                     APDLILTGRIERFFEHDRRKLWTFIPYYSDKLASLFRVNSFKTNGEIHLTLTLLKPTG
                     EVVGSYVGHASFDEDYTPNGEVRPGDRLNRALGQALAQIRDEMFADSTLTKSRTPHPL
                     ALQ"
     gene            complement(5817..6272)
                     /locus_tag="PLS35_13130"
     CDS             complement(5817..6272)
                     /locus_tag="PLS35_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNI19659.1"
                     /translation="MLDVPVLRTNEDFEKGQAPDSIRRAEESMGWAGHLVIVCPLWLG
                     SMPALLKAFFEQVFRSGVAFQYQPDATRAKKLFAGKSARVVVTMGMPAWVYRWWYHVH
                     SLKGLQRNIIQCCGIKPTRATLIGNIESMNEQRRAIWLDEMRGLGERGQ"
     gene            6970..7407
                     /locus_tag="PLS35_13135"
     CDS             6970..7407
                     /locus_tag="PLS35_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI19660.1"
                     /translation="MRATEYFARGCDPNTLVVRQIMEDAVVTIHPTSSAMAVAEILSE
                     HSFGSVPVVDKDGTLRGLVTEFDLLKAVEQERDLRSVLVSDIMTKDVVTTTEETPIMS
                     LIHLLQERHLIRVPVVKGQKLVGMVARRDVVFAYVKARAIYWP"
     gene            complement(7462..>8079)
                     /locus_tag="PLS35_13140"
     CDS             complement(7462..>8079)
                     /locus_tag="PLS35_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNI19661.1"
                     /translation="LLLGYGIIWIWLAINPVNRHDWVLENLLAVGLVAILVLTYRRFT
                     FSLTSYSLIAVFMVLHAIGAHYTYAEVPFGFWLKDIAGLTRNPFDRLVHFSYGLLLVY
                     PLREVLVRLAGVQGSWSYVMPVSSILAQSAFFEVIEAIVAMIVSPELGSMYLGTQGDE
                     WDAQKDMAAAFGGAIVTMVMTAALSAPRNSQVQQPSHRSAPSVAH"
     assembly_gap    8080..8179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8444..9172
                     /locus_tag="PLS35_13145"
     CDS             8444..9172
                     /locus_tag="PLS35_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI19662.1"
                     /translation="MPMPLLDIRSLSVVVDGQVILDRLDLAVQGREIHALVGANGSGK
                     TTLAYVLMGCEGYSPSAGHVLFNGMDLLRLKMHERARLGLTLAWQEPARFEGVTVREY
                     LSLGKPECDPEPALKQVGLDPDRYLTRRMDKALSGGERHRIELASVLSMKPKLAILDE
                     PVAGIDMLSIHHIIEIIRALKASGGSVLLITHQEEVVLIADRASQLCGGRIIFSGSPS
                     EAVDHFRGRSCVRCDGEVCGYVRP"
     gene            9159..10094
                     /locus_tag="PLS35_13150"
     CDS             9159..10094
                     /locus_tag="PLS35_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNI19663.1"
                     /translation="MSDLEELMRVLPRVGAEPSILDDTGIAHVVAHGHHILSHRTVPG
                     LRVDIQETPEAIIGKIVVEAGVTIAQPVHLCFGLAHPTGTQRITMDMQVNEGAQVRVL
                     SHCLFPFVQDGQHRMQAVMTINPGASLTYTEGHYHGPYGGMQVLPHATIKIGKGARYF
                     SDFSLLSGSVGNLDINYLVEVEEDGLAELSAKIFAHQRDRLTLKEAVMLRGERSRSLI
                     KTRVVLEDDATAEITGITEAYAKGTRGHVDCMEVVQGRAHASAIPIVKVFHPEAKVTH
                     EAAIGSVDKKELETLMARGLNPEQAVELIVSGMLR"
     gene            complement(10484..>11262)
                     /locus_tag="PLS35_13155"
     CDS             complement(10484..>11262)
                     /locus_tag="PLS35_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19664.1"
                     /translation="SKRNSMLCNLPATLQYEKLQQLHEAIQLNVDRSPRAFRSGRWGF
                     DTEVARNLLRLGYRIDTSVTPYTSWVQTAGPDFSCCSPQPYGFAPNVHTGRSSNEVLA
                     EIPATIGYLHGEFQACATLADRLNRAPFGRFRVGGLLSRLHLLRKVWLSPEMETPARM
                     MQLVRQMRHQQYEWLNLVFHSSALLEGCGPFVRSRADEQAFLKRLRTFLCAAVKEGIL
                     CVSLSEAARHFIPASGEPAATPDSSSLMAKCPAFSSQPFA"
BASE COUNT         2542 a         3272 c         3090 g         2258 t
ORIGIN      
        1 gtcaacgaat agcgtagata tccttccgta gacggcgttt tcatgatgcc cgacaattct
       61 ccgccgggct ggttgtctcc caacaggaac gagttgggat tcaaatagga actggtcgag
      121 gtgccgccga tcgtcgcggt gtacggcaca cccggtgtgt agtggtacat gacgaacggc
      181 gtattcaacg tcggactgcg tttctcgctg aacggaagat cgagctcaaa ctttccgact
      241 cgcaaattga gcaagtaatt gccgggatcg gatttcaccc cgaaataccg ttccagacgc
      301 atgaggcgca cgaacgcgct ttcaagatct ccatccgacg cgccggtgcc gaaccccgcg
      361 ctcccgagcc caggcgtata caccacaccg aacgcaatat cgcggtgcag cgtgccgaaa
      421 ctcaagaggt ccaggccggt gaaaccaaac cctccggttg tcacaccact gccttccgtc
      481 cgcaactgcg cggcctgata gccgacggtc gcccgaatgg aaaccggcca gaaacccatg
      541 ccgatcccct cgtagaccgg ggagtccgcg tccgatccca attgatatcc gcgatccctg
      601 aagagattgc cgaagtcgtt gagcttcggg aatccgggca cgtggcacac gttacactta
      661 aaatcatact tgcgggcaaa ggccggaatc gcctcggctt tctggaccag cccgctcaat
      721 tcgtaactga tcagccccat caggacgagt atggcgacaa tcatcgccct ggcacgtagt
      781 gtagacatct tcatcggatc agcctcccca tttgctaccg gctgggttga ttgcttattc
      841 cggcggaagt tcatcgccgc cgccggctat gttccattcg acttcgaccg gccggacggg
      901 aaactcaaag cgagattcga cctgggcttt cgaagacacc tctaccggtt gccggatcac
      961 atgaggctcg tcatacacag gcctggaagc actgaacttg acgatattga agccggggtg
     1021 ccaggcgacc agcgtgtagg agccgggagg aatgcctgcg atctcgaatt tcccctgttc
     1081 atcggtcacg gcaaaatagg gatgctcgag ggctgccgcc cacgcattca tatgcacatg
     1141 cacatcacac tgcagccgca agccgacccc ccgtgcccgg cccagcgtgg tcgcgatttc
     1201 ccggttcgcc gtgggcatcg caatattgaa gaggctttgt ttctcgctga agagatgtgg
     1261 attgtggagg atcggttcga aattgatgag cgccacttcc gccgtgcgga cgaagggaat
     1321 catatgtcgt tcgaactggc aggcccgccc cgtgggatgc gaggcgcttt tccccatgcg
     1381 cagcggatag gccgtggctg cggacttccc ccgttctacc cgctcaagat agaccaggac
     1441 ttcgtgaatg ccgttcgtgg ccggatcgat tcgcaaagcc ggtgacggca cgtgcgtgcc
     1501 gcaggagtcc agatccgcaa acactttgag cggcggcaac ttgggaatac tgcccttcca
     1561 gaccgcctga cctttgactg tgccgccatc ggagacgtcg atgatttcat attccgcata
     1621 tccgctgatt gcggtgagaa gccaggcgag gcaggagagg agcacaaggc ggaaaccgcg
     1681 atgtcgtcgt ggagacatga aagacctcat cgacataccg agagccggat ggcccagggc
     1741 ggtgaaaata aaaaagcccg aatctccaca ggagattcgg gctcgagagc tgatcgctcg
     1801 ttggcctcac ttatgtgcgc acggtgggga gcctaatcaa ccctccttcg gcctgtcaag
     1861 tatgcgatgc cgcatgtaac acaggccatg cgttccgctc actcactccg cgagacctcg
     1921 cttcatcagg caatccctcc cagagacgcc gatctatttg aatccgatga ccatggaatc
     1981 cggcccggcc agcggttcaa cctttgtgcg tgtgaagccg accttcttca tccactgccg
     2041 gcaatccgca ccggtgaaat caaatccgcc cggagtctcg atgagcatat tgaggctcat
     2101 caacaaacca aaggcattcg ttttcctggc gtcatcgatc agggcctcat gaacgatcaa
     2161 tgcgcctccc ggtggcagcg ctgcataggc cttgcgcaac agcatcattt tttctttgag
     2221 gtcccagtcg tgcaagatgt gccccatgat gagcacatcc accttcggca gcgagtcctt
     2281 gaagaaatct ccctcgtgaa acgtcatgcg cttctgcagc ttcttgcttc tgatatagga
     2341 ttcaaagacc ggccggacga caggaagatc catgccctgg ccgttgagat gctggtgcgc
     2401 cagcgcaatc tcaaccgcca cgccgccttg agcacaaccg atgtcggcaa acgagtggta
     2461 tcgtttccag ggaaatttcg cggcgatgga tcgggccgtt cccttgctga gtccggtcat
     2521 cgccttgagg aatccttcca atcgctgcgg atccgcgtac agcgtgccga aaaaatcttc
     2581 gccggttttc acttcattct gcggttgacc ggtacgcagc ccctcggtca gtgatcccca
     2641 aaatccatac aggcgggcat tacacatttc cagcatgccg ccgacatacg tcggtttggc
     2701 ccgatcgagg aacagcgcgg tctctggtgt gttggcatag cgtgtcccgt tccgtttcag
     2761 gagcttaagt gccaccaacg catcgaaaaa gtctcgcgca cttcgcggat gcagtttcaa
     2821 tctggccgcc aaggctttcc catcgagttg gcgcttggcc aacagcgtga agagtcccag
     2881 ctccaccgcc gacaatagag tcttggaact ccaaaacccg aacccgattt gcaggatgtg
     2941 atcaggaacc aatggacgac cggccataat gccatccttt cgtgagaaaa attctcgaga
     3001 ggctacgcgg gaaatgtcgc cctgtcaatt tcagctaccg ctccacggtc acggaccgta
     3061 tctctttttc ctacgccgta tcgagagaga tacagcggct cagattgcgc aggaatcgcc
     3121 acgccgtcac agcctacgca gagcgatgga tctgcaattg ggacgacaag gcaggatcct
     3181 tgctagagca tcgcacatct acttatccgc tcatgacgca aaaaggagat cctcgacatg
     3241 gctacgtacc tccacgtgaa ggcagccctg acggcactcg tactgttcag catcatgacg
     3301 gcgaccgccg gtgcagagca atggaacgga ccgcaaactc ccacggggac catctggcgt
     3361 ccgggtaaca tcgcactggg gtttgatccc gccggtcaca gcggcactcc tccggcactc
     3421 gaaatacgcc gccccctttc aggcaccgaa gaggtgctcc tcagcttgcg ttccaccttt
     3481 aaagaggcca ccgctaccgt attggaagtc gacaccaaac gggtctacat gggcggggcg
     3541 gcgaccagaa cctccctggt gccggatggc gtattcgatc tcgccgtagg acgcggcatc
     3601 gccgtggggg tcaacagtgt cacggaacga ctgccgaccg attatcgatt ggtggtgggc
     3661 gggaaggtgc tggctgaaga agtccgcatc aagctgatca aggactgggc ggactatgtc
     3721 ttccatccgg attaccgact ggcggcgctc cctgaagtgg ccacatacat ccagagccac
     3781 cagcatctgc ctgacatgcc aagcgccgcc gaagtcgcag ccaccggaat cagtctcggc
     3841 gacatgcagg cgaaactgct tcgcaagatc gaggaattga cgctgcacgc catcgaacaa
     3901 caccagacca tcgcagcgct gcaggcgcgg ttgcaacagc tggaggaaga ccggtcgtcc
     3961 cagcagctag ggagcaagta gccgcgatcg attcacatcg ttaagagggg gcgcagccat
     4021 gactcaattc accgcactca caacggcgtt gctgctggcg acattgaccc tgccggccga
     4081 ggccgggact accagcctca cgctgaacca agtcatcaag gaaggcaccg ctcgttacta
     4141 cgaccaccct caagagctga ggctggcaga tgggttttgg gctcaggccg gttccgacat
     4201 gacagccacc attcaccttc agcagggata ctacctggat aagacccccc tggataccaa
     4261 ggagaagcaa tacacgtcca ctgacggccg gacatacctg gtcgccggct gcgtaccgaa
     4321 acacctcccc atctcgggac cggtcgggtc agtgtgggtg gcgtgcaacg acctcgatta
     4381 tctcaaggat aaggtcgtcg aaagccgttt cgtgtcgtgt ctggatgtgt tcaccaacgg
     4441 cgtggtgtcc ggcgtctcaa tcaaggaggt caacaatcgg acgtgggaat cgttccggtt
     4501 ctcctgccgc gacatggggc cagacggcag catggtgggc gccgctcaga aatccgactt
     4561 cttattcaac tatgaacgcg aaggcaagct gtacgaaacc acgacggcca gcgggcacct
     4621 caccttcgga atcctcgaag tggccaacgg tctaaccggc accaagagcc tgctccaaat
     4681 cgcccttgaa cacactgccg ccgccgcgct tcaggctgcc ggagtcaatg gacggcacgt
     4741 cgacgacttc acactcagcg acaaagttcc ggacgccttt ggattaggcg gcatgctgag
     4801 ggtcgatcac tggaaatgtc cgcccggcat ggtgatcacc ggtatggcca ttggccataa
     4861 cccggataag aaaggcaatg acacgaagcc agtgtacatt ctggccgaat gccggaagct
     4921 gctgtacacc ccataaggcc ttaccttaaa gccgttgcac gacaggtgat ctgacgcttc
     4981 acatccgcgt ctgtttatcc acttccacct cgttgtgcac tacgagccgc tcccccaact
     5041 ccgggcacgc cggggattca cctagttacg acgtctcgcc atcccggctt ttaatagatt
     5101 atggctacaa tgctccagaa ggcgctgcaa ggactcctgc tcgtcctgct ggtgggctgc
     5161 tcgacaacgt atgagccgca tctcgtgctt cctcctgccg aattcaaccg ctccatcgat
     5221 gcggccgtgg aagtgcatcc ctttgtcgca gctgatgacc ttctatacgg gcgttccaac
     5281 tatggcgtgg ttgctgatga ctatgccaat aagcccccct caaagttgac gcaatcgatt
     5341 acccagcaag tgacggcgga attggcggcc catcgggtgt ttcgccgggt cagcacctat
     5401 gatccggcac cggaccttat cctgacgggt cgaatcgaac ggttcttcga acacgaccgc
     5461 cgtaaactat ggacatttat tccgtactat tccgacaaac tcgccagtct tttccgtgtg
     5521 aatagtttca agacgaatgg agaaattcat ctcacactca ccttgctgaa acccacggga
     5581 gaagtggtgg gatcgtatgt ggggcatgct tcattcgacg aagattacac gcccaatggg
     5641 gaggtgcgtc ccggcgatcg actcaatcgc gcattgggac aggctctcgc gcagattcgt
     5701 gatgagatgt ttgcggactc gaccttgacg aaatcacgta caccccaccc gcttgcgctt
     5761 cagtgatgct tcccctcgag tttgatgaga ccgagcaacc cagaagatcc gcgcagtcac
     5821 tgaccacgct ctcctaatcc ccgcatctca tccaaccaga tcgcacgccg ttgctcattc
     5881 atactttcga tattgccgat gagcgtggct ctggtgggtt tgatgccaca gcattgaatg
     5941 atgttccgct gcagaccctt gaggctatgg acgtggtacc accaccgata cacccatgcc
     6001 ggcatgccca tcgtcaccac gacacgcgcc gactttccgg cgaataactt tttggccctc
     6061 gttgcatcgg gttgatattg gaatgccaca cccgagcgga acacctgttc aaagaatgct
     6121 ttgagaagtg ccggcattga tcccagccat agcgggcaga caatcacgag atgccccgcc
     6181 caacccatgg actcttctgc ccgtctgatc gaatccggcg cttgcccttt ctcaaaatcc
     6241 tcgtttgttc gtaggacagg aacgtccaac attgcgactt cgacccgccg gactgtagac
     6301 caccgtcctc gcactccttt gcatattcgt cagccaatgc atggcaaaaa tgacgagcct
     6361 gtccgtcagg gtgcccctgg ataatggtaa tgcgcttacc catggtgagg cgtgggtgtc
     6421 gcgcttccct ctcgtacgat cggacgaagg gatcgatcca tctcgcggac ggggccctct
     6481 ggtctttgct cgatgctctg cggggctaca gtcggccttt gttgaatctt gggtgtcggc
     6541 ggtgcacaag cggccataga gaagagcagt cccgtcacaa tcacgaaaac gatactcgtt
     6601 atggctcgca ttctccttcc tccctcacca tcttcaatcc tctgcccgac ttcatcgttc
     6661 ctcgctaggc agcgatcaac agtcctcacc cagatgagcg caacaggtat gccagaaccg
     6721 aggcatggcg gagggcagca aatcgagcag aatggctcga tcacgaggcc atcgtgcgat
     6781 ggcgctgcgg atattttccg cagtacgagg accaggagct ggcgcgaatg tccacagcag
     6841 agaacatccc tgactccgga agacggctgc tgcttttatt ttttgcgacg gagggggaga
     6901 atgagcaggg gcatgcgccg aaggctggtg ccctgtcgcc ttattccaac cgaacagaag
     6961 gagggtccta tgagggcaac agaatatttc gctcgcgggt gtgatcccaa cacattagtc
     7021 gtcaggcaaa tcatggagga tgccgtcgtc acgattcacc ccacatcgag cgccatggcc
     7081 gtcgcagaaa tcctgagcga acacagcttt ggaagtgtac cggtggtcga caaggacgga
     7141 accttgcgtg gcctcgtgac cgagtttgat ctgctcaaag ccgtcgagca ggaacgagat
     7201 ctccggagcg tgttggtgtc ggacattatg accaaagacg tggtgacgac gaccgaagag
     7261 acgccgataa tgagtctgat tcatctgctg caggagaggc acctgatccg agtgccggtc
     7321 gtgaaggggc agaaactagt cggcatggtg gcacggcgag atgtcgtctt tgcctatgtg
     7381 aaagccaggg caatctactg gccgtaatgc cgctctagga cagagcctct cgaacggcat
     7441 ggcgcttcac gcgccacagc cctaatgggc gacgctcggc gcagaacggt gtgacggctg
     7501 ctgcacctgc gaattgcgag gcgcagatag tgcggcagtc ataaccatgg tgactatggc
     7561 gccaccaaag gccgcggcca tgtccttctg agcgtcccat tcgtcgccct gcgttcccag
     7621 atacatactg cccaattccg gactgacgat catcgctacg atcgcttcaa tcacctcaaa
     7681 gaatgcgctc tgtgcaagaa tgctgcttac cggcatcaca taggaccaac tcccctgcac
     7741 tcccgccagc cgcaccagca cttcccgcaa aggatagacc agcaagagac catacgaaaa
     7801 atggacaagt ctatcgaaag gattccgggt tagtcccgca atgtctttga gccagaaacc
     7861 gaatggcact tctgcatagg tgtaatgagc accgatggca tggagcacca tgaatacggc
     7921 gatgagggag taggacgtga gagaaaatgt aaaacggcgg taggtcagga ctagaatcgc
     7981 gacgagtccg acggccaata aattttctaa cacccaatca tggcgattga ccgggttgat
     8041 ggccagccag atccagatga tgccataacc caataacaan nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnng gtacaggagg gacgtccaat caaactcccg aaaggattga
     8221 attactctac tggatgatct tcggtcctgc aacagtcctg ccgggccgtt cgcccccata
     8281 acatagactc gcagccggcc ctcttgcact tggagaagta cctagcacaa gcgggcgtgc
     8341 tgctctgtcg cacaaggcca cgttcaaaga ccgatggcga ggggcatatc gtgattcggg
     8401 gcacagctga tgcatctcga ttggatcaat gacggatgtt gctatgccaa tgcccctgct
     8461 ggatattcgg agcctgagcg tggtcgtcga tggacaggtg attctcgatc gactggacct
     8521 cgcggttcaa ggccgggaaa tccatgccct agtcggtgcc aacggctcgg gcaaaaccac
     8581 ccttgcctac gtcctgatgg gttgtgaagg gtactcgccg agtgcgggac atgtgctatt
     8641 caacggcatg gaccttctgc gtctgaaaat gcatgaacgc gcccgattag gattgacgct
     8701 tgcctggcag gagccggctc gatttgaagg cgtgacggtg cgcgaatatc tgagtttagg
     8761 caaaccagaa tgcgatcccg aaccggcttt gaagcaggtc ggtctggatc cagaccgtta
     8821 tctcacacgg cgaatggata aggcactgag cgggggcgaa cgtcaccgaa tcgagctcgc
     8881 atcagtcctc tccatgaaac cgaagttggc cattctcgac gagccggtgg caggaattga
     8941 tatgctttcc atccatcaca ttatcgaaat catccgcgcc ctgaaggcaa gcggcggctc
     9001 cgtcctcctg atcacacacc aggaagaagt ggtcctcatt gctgatcgag cctcccagct
     9061 ctgcggcgga cgcattattt tctcgggcag cccgagtgaa gccgtcgatc atttccgcgg
     9121 gcgcagctgt gtgcgatgcg atggagaggt ctgcgggtat gtcagacctt gaggaactca
     9181 tgcgcgtctt gcccagggtg ggcgctgagc cgtcgatcct cgacgacaca ggcattgccc
     9241 atgtcgtggc ccacgggcat cacatcctga gccatcgaac cgtcccgggc cttcgcgtcg
     9301 acattcaaga aacgccggag gccatcatcg gcaagatcgt cgtcgaagca ggtgtcacca
     9361 tcgcccagcc cgtccacctg tgtttcggcc tcgctcatcc caccgggaca caacggatca
     9421 caatggatat gcaggtgaac gaaggggcgc aggtgcgagt gctctctcat tgcctctttc
     9481 ccttcgtcca agacggccag catcgcatgc aagccgtgat gacaatcaat cccggcgcgt
     9541 cactgaccta tactgagggg cactaccatg gtccctacgg tggcatgcag gtcttgccgc
     9601 atgccacgat caagatcggc aaaggggccc gttatttctc ggatttctcc ttactctccg
     9661 gttcagtggg gaacctcgat atcaactacc tcgtcgaagt ggaagaggat ggactcgccg
     9721 aactcagcgc caaaatcttc gcgcaccaga gggatcgtct cacactaaag gaagccgtca
     9781 tgctgcgcgg cgagcggtca cgaagtctga tcaaaacccg tgtagtgctg gaggatgatg
     9841 ccacggcaga gatcaccggc attactgaag cctatgcaaa gggaactcgc ggccacgtag
     9901 attgcatgga agtcgtccaa ggcagagctc acgccagcgc aatcccgatc gtcaaggttt
     9961 ttcacccgga ggcgaaggtt acacacgaag cagcgatcgg cagcgtggat aagaaggagc
    10021 tcgaaacact gatggcgcgc ggactgaatc cggagcaagc ggtcgaactg atcgtaagcg
    10081 gaatgcttcg ctagcctcga cgtctcacga acggtcggac tgtcttgatc aagcggaagg
    10141 atcggggggg cactccggat cttcgatcac tgctccgcat tcgaggcatc gataacgagg
    10201 agggtcgtcg tcgtgctccg agtggatgcg atcgatgagt cgcatgtgac ggcaggtgag
    10261 ggtccgtgtc atggtcggat caagttgagg actacgcata acgacctccc cggatcacgc
    10321 ttgactatgt acctgagaaa atgcgcccca accctagcag atttctcaag acttgacacg
    10381 acgaattttc cgcagatgga agccagacat atgctgacac aataattggt ggccttatgg
    10441 ctttcgccgc tacatataga gatcattatt ctctgcgagc ggttcacgca aagggctggg
    10501 aggaaaaagc aggacatttc gccatcaaag atgaggaatc aggcgtggcc gccggctcac
    10561 ccgaagctgg aatgaagtgt cgtgcggctt ctgaaagcga gacacacaga atcccttctt
    10621 tcaccgcagc acaaagaaat gtacgtagcc tcttcagaaa tgcctgctcg tccgctcgag
    10681 aacgcacgaa tggtccgcag ccttccaaga gcgctgaaga atggaaaaca agattcagcc
    10741 attcatactg ttgatgccgc atttgacgga cgagttgcat cattctggca ggagtctcca
    10801 tctccggaga aagccacacc tttcgcaaga gatgcaagcg cgacaacaat cctccgactc
    10861 taaacctgcc aaatggggca cggttgagcc ggtctgccaa tgtagcgcag gcctgaaact
    10921 ctccatggag atacccgatc gtggccggaa tttccgccag cacctcgttc gaggagcggc
    10981 cggtgtggac attcggcgca aatccataag gctgaggaga acagcaggag aagtccggac
    11041 cagccgtctg gacccacgag gtgtacggag tgacagacgt atcgatacgg tatccaagac
    11101 gaaggagatt gcgcgccacc tccgtgtcaa atccccaacg gccgcttcgg aacgccctag
    11161 gagatcgatc aacgttgagc tgaatcgctt catgcaactg ctgcaatttt tcgtattgca
    11221 gggtcgccgg gaggttgcag agcatgctat tgcgtttact ta
//
LOCUS       DAOQEW010000125        11242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1751_length_11242_cov_8.728614, whole genome shotgun sequence.
ACCESSION   DAOQEW010000125 DAOQEW010000000
VERSION     DAOQEW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_11242_cov_8.728614"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..610)
                     /locus_tag="PLS35_13160"
     CDS             complement(170..610)
                     /locus_tag="PLS35_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19665.1"
                     /translation="MSDIRIDQNGAADLIAASNGSLTVSLPIKLIRRGKCMAVTLPDG
                     TTVQPRPWDDTPTPIQQALARGHRWLEMLQSGQAQSLTEVAELEGMDRAYVSRMVNLT
                     TLAPDIVAAILDETLPDHVTLFDLASGTPLLWDDQRALLRKKQS"
     gene            complement(607..1953)
                     /locus_tag="PLS35_13165"
     CDS             complement(607..1953)
                     /locus_tag="PLS35_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNI19666.1"
                     /translation="MNEILKRRQRCAVYCRVSTDERLDQEFNSIDAQREAGHAYIASQ
                     RSEGWIPVADDYDDPGFSGGNTERPALKRLIADIEEGKIDIVVVYKIDRLTRSLADFS
                     KMVEVFERQGVSFVSVTQQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKIAASKR
                     KGMWMGGVPPIGYDVENRRLVPNGRESKLVRFIFERFLELGSSTKLVKELQLEGATTK
                     SWTTQDGNHRPGKLIDKTSVYQILNNRTYLGELRHKEQWFKAEHPPIIERSVFDAVQA
                     ILEVNPRVRANNTRAKIPFLLKGVVEGHDGRALTPAWTRKGNGRLYRYYMHTRENKEF
                     AGASGLPRLPATELEATVVEQMRRVWRNPEMISRVSEHAMRMDPDTNEAHVWIAMAQI
                     DKIWEQLFPAEQHRIVRLLIEKVIVTPHNLEVRFRPNGLEMLAREVKPTTTEEVAA"
     gene            complement(1950..2450)
                     /locus_tag="PLS35_13170"
     CDS             complement(1950..2450)
                     /locus_tag="PLS35_13170"
                     /inference="COORDINATES: protein motif:HMM:NF022598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HNI19667.1"
                     /translation="MNTAPASHTTSVSVTAQITQLPDLSMSDIKSIWKELFHADTPTH
                     NRQFLERRIAYRLQEIEFRKVDRSLVDRNKRRIQQILDSGKNKKLDRDIRMMAGTVLT
                     REYQGHEYRVMATTDGQYEFEGRLYQSLSRIAKEITGTAWSGPLFFGLKTYAGKKPAA
                     KKGGKK"
     gene            2483..2743
                     /locus_tag="PLS35_13175"
     CDS             2483..2743
                     /locus_tag="PLS35_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19668.1"
                     /translation="MNALVTGEAKLKLALSDHICGLSTACCAQADKPSCQQGCGLGST
                     LLCGHHLGRVVVDFMHWLPILSVQLAQGKIVRGGHSQCQEGI"
     gene            complement(2795..3703)
                     /locus_tag="PLS35_13180"
     CDS             complement(2795..3703)
                     /locus_tag="PLS35_13180"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNI19669.1"
                     /translation="MTEHKKPMAEANRISSMLNAVLGKERFPVKVDELAVEYSRQCFP
                     DSPIDKVEGADLEDFDGLLKAKKDRSKWLILYNSAVSSEGRKRFTIAHEFGHYILHRH
                     QQTEFACGDDDIESGDSRDIESEADLFASTLLMPLDDFRRQVDGQKINFDLLGHCADR
                     YGVSLTAAALRWTEIAEKRAILIASRDDHMLWAKSNLAAVKSGAYFPTRKNTIELPPE
                     ALAHSNNAWLSNQEQTTRAQVWFYREPQSMPITEMTRVAGQYDYTLTLLLMPDAEWQR
                     PWHGDEESEEDTFDRFIRNGQFPSKK"
     gene            complement(3700..4020)
                     /locus_tag="PLS35_13185"
     CDS             complement(3700..4020)
                     /locus_tag="PLS35_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI19670.1"
                     /translation="MPSPLGNKIRELRKQKKLSLEQLAELTESSKSYIWELENKDDPK
                     PSAEKTAKIAAVLEVTAEFLLSDSVTTPDEEVADEAFFRKFKKLSEPDKKKIRKILDA
                     WEDE"
     gene            4229..5461
                     /locus_tag="PLS35_13190"
     CDS             4229..5461
                     /locus_tag="PLS35_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19671.1"
                     /translation="MNDAENLSKLLGHLPPAVFRSFMAAKFSLEMPDLDKKAAKKDQR
                     AGMEAVLAALDVSARQRIEEVAEQIILLSDGPGQDVLDGFSQEIFDDEARAQFEAIRS
                     QYERALWIHLNEGDLFEEALNARQADIFRQSPSCYSGYMAPKDLSVLEDSAARQAFHN
                     AVAGQLGCPVDEVAVQVFKRLRPDTQTGEEVDLYQVSVHHNRPPEIVDCVQASELVQQ
                     EVVRAVTSHITYEPSNGHLEVLSKFTDGREALARIVSDHLLQSPITGEKIPLKQYDYQ
                     SLNAPRTFDLTGETTVASVKVVELGYTSTDFRTLVVKIGFKDTDDIHTAARSLIGPGF
                     TFQGRRLTYAKLSIRLKKVGKDRARTITVILRDENKCNIKTKREKDRVLCDRLLAKWQ
                     LVKEIGDVTNDPIYALAA"
     gene            5451..6431
                     /locus_tag="PLS35_13195"
     CDS             5451..6431
                     /locus_tag="PLS35_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19672.1"
                     /translation="MLLDLLDLFEQSNSCSLDADGNRLHGVSGGRIEASSSLDPKTVQ
                     AWMPRVGYLSEIEVPCGDGRAHVELCETDDPGSYEYRCPETFRRKRVSADHVAVYDMD
                     AAKLLNLLSDLLNIPQVKRAGIPAPRIERKLWRLGEARIGPVMAPVWLARGMDVNVDE
                     VFQSLLDTRLPEQGLVLCHGRELPRVIRPPRNYRVAYLHDALVDYSPSPCMDVHYLER
                     VLTSDEDGIKPSALPVDFANGVLRIRTKTETWVIKGEKQLKAVAYMYEQAQLGRWELD
                     ASEILAAAYPERRTEESRKGLKMQNLFSGNEMWREFIVNPEKGKYAFKTL"
     gene            6615..6827
                     /locus_tag="PLS35_13200"
     CDS             6615..6827
                     /locus_tag="PLS35_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19673.1"
                     /translation="MSVKHLNQRQLGDRWDVSEATLERWRSEGIGPVFLKLQGRVLYR
                     LEDVEAFEAESLRKSTYEPVNAGGAA"
     gene            6824..7294
                     /locus_tag="PLS35_13205"
     CDS             6824..7294
                     /locus_tag="PLS35_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19674.1"
                     /translation="MNRLTPHQVLAMPAGDLALQTSESLFQLKNDAADLQALAKAVVD
                     HLDRALDLKYSKQAHALRLAAGKDTGVVHFDDGHVQVTADLPKKVDWDQKKLADLVRR
                     MTANGDNPAEYVEISYRVSETKFNAWPETLKSAFAPARTLKTGKPGFRLALLQE"
     gene            7300..7680
                     /locus_tag="PLS35_13210"
     CDS             7300..7680
                     /locus_tag="PLS35_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19675.1"
                     /translation="MKFPSFKKVSRQVAERKVQEAAQAKPLPLMTWLRKNLSLYSKHL
                     PEAIRIPAMGAARPDEVVRLLPDATIDDLAFAIQGSEEESSEVLRRTGALKELYEAAR
                     KRGALGTTTIAEAFAAAVDEEVRK"
     gene            7677..8561
                     /locus_tag="PLS35_13215"
     CDS             7677..8561
                     /locus_tag="PLS35_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19676.1"
                     /translation="MMLPIITADQRLAERRGIKGVLVGKSGIGKTSQLWTLNPIATLF
                     MDLEAGDLAAEGYQCDTIRPRTWQDCRDFAVFIGGPNPALRDDQPYSQAHFDAVCGRF
                     GDPAVLDKYETLFVDSITVAGRLCLQWCKGQPQAYSEKTGKPDSRGAYGLMGQEMIGW
                     LTHLQHTRRKNVWFVGILNEALDDFNRRVFSLQIDGSKTGLELPGIVDEVITLAEIKG
                     DDGSSYRAFICQTLNTWGYPAKDRSGRLDLIEEPHLGRLMEKIAAPGRPALDRLDFAR
                     PATAAVEPSLNANVSQES"
     gene            8564..9160
                     /locus_tag="PLS35_13220"
     CDS             8564..9160
                     /locus_tag="PLS35_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19677.1"
                     /translation="MSFFDFNSASEQTSFDLIPKGALVRVRMTVKPGGFDDASQGWTG
                     GYATRNDNTGSVYLNCEFVVMEGQYARRKMWSLIGLYSPKGPEWSNMGRTFIKAVLNS
                     ARAIHPNDNGPAAQNARRINGFADLDGIEFLGKVDWEKDQNGQDKCVIKSAVTPDHKD
                     YAALMGGASQPAPAASAPNAYAQATGRSPVPGRPSWAQ"
     gene            9176..10849
                     /locus_tag="PLS35_13225"
     CDS             9176..10849
                     /locus_tag="PLS35_13225"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI19678.1"
                     /translation="MMLRPRQSLLVDRTLSALDAYGNTLAVAPTGSGKTIMLSAVAGR
                     VLVEPDAKACILAHRTELTGQNRAKFERVNPGMSTSVFDANEKSWSGQATFAMVQTLS
                     RLSHLEQMPTLDLLVIDEAHHSSSPSYRAVIDAVLARNPKAGICGLTATPNRGDGKGL
                     RDVFSNVADQITLGEMIAAGHLVSPRTFVIDVGVQDALKNVRKTAMDFDMDEVASILD
                     KRLITEAVIKHWKEKASSRKTIIFCSTVAHAQNVCDAFIDAGINSVLIHGELSDGDRK
                     SRLAEYEHGRAQIVVNVAVLTEGYDYTPTSCVVLLRPSSYKSTFIQMVGRGLRTVDPE
                     EFPGVIKSDCLVLDFGTASLMHGSLEQEVNLDGHLHDGPAPTKDCPDCGAVVPLACME
                     CPLCGYVWERQPQDLGVLADFVMSEIDLLKRSNFRWCDLFGSDDALMATGFNAWGGIF
                     FLNGRWHAVGGGQGMQTHLLAVGERTVCMAKADDWLNDHESADSAHKTRRWLNEPPTP
                     KQLQYLPQALRADFGMTRYQASALLSFQFNKTSIQRLVMAANDSYREAA"
     gene            10846..>11242
                     /locus_tag="PLS35_13230"
     CDS             10846..>11242
                     /locus_tag="PLS35_13230"
                     /inference="COORDINATES: protein motif:HMM:NF039802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6511 domain-containing protein"
                     /protein_id="HNI19679.1"
                     /translation="MKCIVCHRQAKGYGWFNTRRKRGDPQRYSDQWVFCSRRCQESFS
                     KLMNKTEGQMIDPSDMEIAAMRACLAPLGEYVGEIGMQRPLADYSRDEVLMLIDVVVT
                     AYQDCMVAEHERMAAKDRAFFEERLARQGL"
BASE COUNT         2245 a         3226 c         3334 g         2437 t
ORIGIN      
        1 gatgaacggg cccaggtaac ctggccacat atcccatttg ccgccctctg gaatgtcgtc
       61 cagttggaag aggcgcttta atatttggcg atcgcggacg gattcgtaat ggctgcacat
      121 cacgccatac tatcaacgcg cgtatccgtt cagaaagccc gacgcagcgt cacgactgct
      181 tttttctcag cagtgcgcgc tggtcgtccc acagcagcgg ggtcccggat gccaggtcaa
      241 acaaggtcac atggtcaggt agtgtctcgt ccaggatggc tgccacgatg tctggtgcca
      301 aggtcgtcag attgaccatg cggctgacgt aggcacgatc catcccctcc agttccgcga
      361 cttcagtcag gctttgcgcc tgcccggact ggagcatttc cagccagcga tggccgcgcg
      421 caagcgcttg ttgtatgggg gtgggcgtgt catcccaggg gcgcggttgg actgtcgttc
      481 cgtcgggcaa cgtcaccgcc atgcatttcc ctcgacggat gagcttgatt gggagcgaca
      541 ctgtcagact cccgttgctt gccgcaatga ggtcggctgc gccattctgg tcgatacgaa
      601 tgtcgctcat gctgccacct cttctgtggt cgttggcttc acttcccgtg ccaacatttc
      661 cagtccattc gggcggaagc gaacctccaa gttgtgcggc gtcacgatta ccttttcaat
      721 gagcaggcgg acaatgcgat gctgttcagc agggaacagc tgctcccaaa tcttgtcgat
      781 ctgcgccatc gcgatccaaa cgtgggcctc gttggtgtcc gggtccattc gcatggcgtg
      841 ttcgctgacg cgtgaaatca tttccggatt ccgccagacg cgacgcatct gctcaacgac
      901 ggtggcctcc agttcggtgg ccgggagccg tgggagtccg gatgcgccgg cgaattcttt
      961 gttctcccgg gtgtgcatgt agtaccgata aagtcggcca ttcccttttc gggtccaggc
     1021 tggtgtcaac gctcggccgt cgtgaccctc gaccacgcct ttgaggagga aggggatctt
     1081 ggcgcgcgtg ttgttggcac gcactctcgg gttgacctcg aggatggcct gcacggcatc
     1141 aaaaacgctt cgctcgatga tcgggggatg ttcggctttg aaccactgtt ctttgtgccg
     1201 cagttcgcca aggtaggtgc ggttgttgag gatctgatag acgctggttt tgtcgatcaa
     1261 ttttcccgga cggtggttgc cgtcctgcgt cgtccatgac ttggtagtgg ctccctccag
     1321 ttggagctcc ttgaccagct tggtgctaga gccgagctcc aggaagcgtt caaagatgaa
     1381 ccggacgagt ttggattcgc ggccgttggg taccaagcgg cgattttcaa cgtcgtagcc
     1441 aatcggtgga acgccgccca tccacatccc cttgcgcttg gatgcagcaa tcttgtcgcg
     1501 gatgcgttcg ccggtgacct cccgttcgaa ctgggcaaac gacagcaaga cattcagcat
     1561 caagcgcccc atcgacgtgg tggtattgaa ctgctgcgtc actgacacaa atgacacgcc
     1621 ctgtcgctcg aacacttcga ccatcttgga gaagtcggcc aggctgcggg tcagtcggtc
     1681 aatcttgtat acgacgacga tgtcgatctt gccttcttcg atgtctgcga tcagacgttt
     1741 cagcgcaggg cgttcggtgt tgccacccga aaaacctggg tcatcgtaat catctgcgac
     1801 gggaatccac ccctctgatc gctgactggc gatgtaggca tggccggctt cacgttgcgc
     1861 gtcgatcgag ttgaactcct gatccaggcg ttcgtccgtg gagacacggc aatagaccgc
     1921 gcatcgctgg cgccgcttga gaatttcgtt catttcttgc cccctttctt ggcggctggt
     1981 tttttgccgg cgtaggtctt caagccaaag aacagtggcc ctgaccatgc tgttcccgtg
     2041 atctccttgg caatccgaga caggctttga tacagccgtc cttcgaactc gtattggcca
     2101 tccgtggtgg ccatgacgcg gtactcgtgg ccttggtatt ccctggtgag tacggtgcca
     2161 gccatcatcc gaatatcgcg atccagcttc ttgttcttgc cagagtcgag aatttgctgg
     2221 atgcgtcgtt tgttgcggtc gaccaaactt cggtcgactt tccgaaactc gatctcttgc
     2281 agccgataag caatgcgccg ctctaggaac tgccggttat gggtcggcgt gtcggcgtgg
     2341 aacagctcct tccagatgga cttgatgtcg gacatcgaca gatccggaag ttgtgtgatt
     2401 tgagccgtca ccgagactga ggtggtgtgg ctggcggggg cggtgttcat cgtgactccg
     2461 tggtgttgtt gtcggggtct gtatgaacgc gctggtgacc ggagaagcca agctcaaact
     2521 cgctctttcc gaccacattt gtggactttc tactgcctgc tgcgcgcagg cggacaagcc
     2581 ctcttgccag cagggctgcg gcctcggttc gacgctgctc tgcggtcatc acctcgggcg
     2641 ggtggtggtt gatttcatgc actggttacc gatcctttct gtacaacttg ctcaaggcaa
     2701 aattgtccgt gggggtcatt cacagtgcca ggagggaatt tagggctcct gattgcccgt
     2761 tagggctgat gcgaaaagcg cagcaaaaat ccggctattt tttcgatgga aactggccgt
     2821 ttcggatgaa ccgatcgaag gtgtcttctt cagattcttc atcgccatgc caagggcgct
     2881 gccactctgc atcgggcatc agcagcagcg tcaaggtgta gtcgtactgc ccggccaccc
     2941 gtgtcatttc ggtgatgggc atgctctgtg gctcgcggta aaaccaaacc tgcgcacggg
     3001 tggtttgctc ctggtttgac agccatgcgt tgttgctatg cgccagagcc tctggtggga
     3061 gttcgatcgt gttcttccgg gtcgggaagt acgccccgga tttcacagca gccaggttcg
     3121 actttgccca cagcatgtgg tcatcgcggc tggcgatgag gatggcccgt ttttcggcaa
     3181 tctctgtcca tcgtaaggcg gcggctgtga gcgacacccc gtagcggtcg gcacagtgac
     3241 caagcaagtc gaagttgatt ttctgcccgt cgacctgtcg ccggaaatcg tccaagggca
     3301 tgagcagggt tgaggcaaac aggtcggctt ccgactcgat gtcgcgactg tccccggatt
     3361 caatatcgtc gtcgccgcac gcaaattcgg tttgttggtg gcgatgcagg atgtagtggc
     3421 cgaactcatg cgcgatcgtg aagcgcttgc gtccttccga cgagacagcg ctgttgtaga
     3481 ggatcagcca ctttgatcga tcctttttcg ccttcaggag accgtcgaag tcctcaaggt
     3541 cggcgccttc caccttgtcg atgggcgagt cgggaaagca ctggcgggaa tactcgacgg
     3601 ccagttcgtc gaccttgacc gggaaccgct ccttacccag cacagcgttg agcatgctgg
     3661 aaatacggtt ggcctcggcc atgggtttct tgtgctcagt cattcatctt cccaagcgtc
     3721 caggatcttg cggattttct ttttgtccgg ttccgatagc tttttgaact tgcggaaaaa
     3781 cgcctcgtcg gcgacttcct cgtcaggggt ggtcacgctg tcgctcagca agaactcagc
     3841 agtgacttcc agcacggccg cgatcttggc agttttttcg gcagatggtt ttgggtcgtc
     3901 cttgttttcc agttcccaga tgtagctctt gctggactcg gtgagttcag ccagttgctc
     3961 aaggctgagc ttcttttgct tccgcagttc gcggatcttg ttgcctagag gggacggcac
     4021 tgttttctcc tcgttgatcc gatttagaac gcaaatgtta ccaccagacc gaacgaaaac
     4081 gtaactgctt gacaaaccca taagcaacca gaaataatca cgacaagttc ggcgtaccga
     4141 acgaaattgg tcagatggct cctgctgtac ggagagggtg ttcgatctgg cgctcacatg
     4201 gtgaacctct tccgtaagga gaatttcgat gaatgatgca gaaaacctgt ccaagctgct
     4261 gggccacctg cccccggcgg tgttccggtc tttcatggcc gctaagttca gtctggaaat
     4321 gccggatctg gacaagaagg ccgccaagaa ggatcaacgg gctggcatgg aggcggtgct
     4381 ggcggccctc gacgtgagcg cgcgacaacg gatcgaagag gttgccgagc agatcatcct
     4441 gctgtccgat ggacctggac aggacgtact ggatggcttc agccaggaaa tctttgatga
     4501 tgaggcacgg gctcagtttg aggcgattcg cagtcagtac gagcgtgcac tgtggattca
     4561 cctcaacgaa ggggatctgt tcgaggaagc gctcaacgca cgtcaggctg acatcttccg
     4621 tcagagccca tcctgctact caggctacat ggcaccgaaa gacctgtctg tcctcgagga
     4681 ttcggcggct cggcaggcat ttcataacgc tgtggccggt caacttgggt gcccagtcga
     4741 tgaggtagcg gtccaggtct tcaagcgttt gcggccggac actcagactg gcgaggaggt
     4801 tgatctgtac caggtgagcg tccaccacaa tcgcccgcct gagattgttg actgcgttca
     4861 ggcgagcgaa ctggtccagc aggaagtcgt gcgtgccgtg acatcacaca tcacctacga
     4921 gccgtccaat ggtcacctgg aagtgctgtc gaaattcacg gatggacggg aggcattggc
     4981 gcgcatcgtc tctgaccatc tactgcagtc cccgatcacg ggcgaaaaga ttccgctgaa
     5041 gcaatacgac taccaaagcc tgaatgcgcc gcgcactttc gatctgaccg gggaaaccac
     5101 ggtcgcttcc gtcaaggtgg tcgagttggg ctacacgtcg acggacttcc ggacattggt
     5161 ggtgaaaatc ggcttcaagg acaccgacga catccacacg gcggcgcgat ccctgattgg
     5221 ccccggattt acgttccaag gccgacgcct gacctacgcc aagttgtcga tccgcctgaa
     5281 aaaggtgggg aaggatcgcg ccaggaccat caccgtcatc ttgcgcgacg agaacaagtg
     5341 caacatcaaa accaagcgcg agaaggaccg cgtgctgtgc gaccgtttgt tggccaagtg
     5401 gcaactggtg aaggaaatcg gcgatgtcac gaacgatccc atctacgcgc ttgctgcttg
     5461 atctcctgga cctgtttgag caatcgaaca gttgcagcct ggacgctgac ggaaaccggc
     5521 tgcacggagt ctccggggga cgcatcgaag catcatccag ccttgacccg aaaacggtgc
     5581 aagcctggat gccccgtgtt ggttacttga gcgagatcga ggtgccgtgt ggcgatggcc
     5641 gcgcccatgt tgaactttgc gaaacggatg atccgggcag ctacgagtac cgttgcccgg
     5701 agacatttcg ccgtaagcga gtcagtgcgg atcatgtggc ggtgtacgac atggatgccg
     5761 ccaagctgct caatcttttg tctgaccttc tgaacatccc gcaggtaaag cgcgcgggga
     5821 ttccagcgcc aaggattgaa cgaaagttgt ggcgactggg tgaggcacgt attggaccgg
     5881 tgatggctcc ggtatggctg gcgcgtggga tggacgtcaa cgtagacgag gtgtttcagt
     5941 ccctgctgga tacgcggctt cccgagcaag ggttggttct ttgtcacggg cgcgagctgc
     6001 cccgcgtgat tcggccgccg cgcaactacc gagttgccta cttgcacgat gcactggtgg
     6061 actactcgcc gtcaccatgc atggacgtgc attacctgga gcgggtgctg acctccgatg
     6121 aggatggcat caagccgagt gcgttgcccg tcgactttgc aaacggcgtc ctgcgaatca
     6181 gaacaaagac cgagacgtgg gtcatcaagg gcgagaagca actcaaagcg gtggcgtata
     6241 tgtacgaaca ggcgcagctc ggacgatggg agttggatgc cagtgaaatc ctggctgctg
     6301 cttatccgga acggcgcaca gaagagtcgc gcaagggatt aaaaatgcag aacctcttca
     6361 gcggcaatga aatgtggcgc gagttcatcg tcaatcctga aaaaggcaaa tacgcattca
     6421 agacgctttg attagcacca gccagcacca gtaccagaca ccgccttcgg gcggtttttt
     6481 catttcaggg gccagtaaat ccccccgttt tccgttgccc atacatcagc ccatacatag
     6541 ggcggatcga cccccataca cgccaaatcc gatcatttcc tcacgttttc gcaactacct
     6601 gaaaggagaa aaacgtgagc gtaaaacacc tcaaccagcg tcaattgggc gaccgttggg
     6661 acgtcagcga agccactctg gagcgttggc gttctgaagg aatcggaccg gtctttctca
     6721 agctgcaggg ccgtgttctc tatcgcttgg aggacgtcga agccttcgag gccgaaagcc
     6781 tccgcaagag cacctatgaa cccgtcaatg cgggaggtgc ggcatgaatc ggctgacacc
     6841 ccaccaagtt ctggccatgc cggccggcga tctggcactc cagaccagcg agtcgttgtt
     6901 ccaactcaag aacgacgccg ctgatctaca ggccttggcc aaagctgtcg tcgatcacct
     6961 cgatcgcgcc ttggacctga agtactcgaa acaagcacat gccctgcgcc tggctgcagg
     7021 caaggacacc ggcgtcgtgc atttcgatga cggccatgtt caggtcactg ccgatctccc
     7081 caaaaaagtc gattgggatc agaaaaagct ggccgatctc gtgcgccgga tgacggccaa
     7141 cggggacaac cctgccgagt acgtcgagat cagctaccgg gtctcagaaa ccaagttcaa
     7201 cgcctggcca gaaacgctga aaagcgcgtt tgcaccggcc cgcaccctca aaaccggcaa
     7261 gcccgggttc cgtcttgcat tgcttcagga gtaaccagca tgaaatttcc atcgttcaaa
     7321 aaagtgtccc gtcaggtggc cgaacgcaaa gtacaggagg cagcgcaggc caagcccctg
     7381 ccgctgatga cttggctgcg caagaacctg tctctgtact cgaagcatct gccggaagcg
     7441 atccgcattc ctgcgatggg ggcggcgcgc cctgatgaag tggtccgttt gctgccggat
     7501 gcaacgatcg acgatctcgc attcgccatc cagggcagcg aagaggaaag cagtgaagtt
     7561 ctgcgtcgca cgggtgcctt gaaggagctg tacgaggcag cacgtaaacg gggcgctttg
     7621 ggtactacga cgattgctga agcatttgcc gctgcagtcg atgaggaggt gcgcaaatga
     7681 tgctgcccat cattactgca gatcagcgct tggcagagcg tcgtggcatc aagggtgtcc
     7741 tggtcgggaa gtcgggcatt ggcaagactt cccagctgtg gacgctcaat ccgatcgcca
     7801 cgctgttcat ggatttggaa gccggcgatc tcgcggctga aggctaccag tgcgacacca
     7861 tccgtcctcg cacatggcaa gactgccggg acttcgcggt gttcatcggt ggcccaaacc
     7921 cggcgctgcg tgacgaccag ccctacagcc aagcgcactt cgatgcggtg tgtggtcgct
     7981 ttggtgatcc tgctgttctg gacaagtacg aaaccttgtt tgtggacagc atcaccgtgg
     8041 ccggtcgctt gtgcctgcaa tggtgcaagg gacagccgca ggcctattcc gagaaaaccg
     8101 gcaaacccga cagccgtggc gcctacggtt tgatgggcca ggaaatgatc ggctggctga
     8161 cccatctgca gcacacccgg cgcaagaacg tctggttcgt cggcatcctg aacgaagcct
     8221 tggacgattt caatcgccgc gtcttctcac tgcagatcga tggttccaag accggccttg
     8281 agctcccggg catcgtcgat gaggtcatca ccctggctga gatcaagggt gatgacggca
     8341 gcagctaccg cgccttcatc tgccaaaccc tgaacacctg gggctatccg gccaaagatc
     8401 ggtcgggccg cctcgacctg atcgaggaac cccacctggg tcgcttgatg gaaaagatcg
     8461 ccgcgccggg tcggcctgca ctcgatcgcc ttgatttcgc ccgtcccgcg accgccgccg
     8521 tcgaaccctc tctgaatgcc aacgtttccc aggagtcctg atcatgtcct tcttcgattt
     8581 caattccgct tccgaacaaa cttccttcga tttgattccg aagggtgctc tggttcgtgt
     8641 ccgcatgacg gtcaaacccg gtggcttcga tgatgcctcc cagggttgga ccggcggcta
     8701 cgccacccgc aacgacaaca ccggttcggt ttacttgaac tgcgagtttg tcgtgatgga
     8761 agggcagtat gcgcgccgca agatgtggtc gctcatcggc ctgtacagcc cgaaggggcc
     8821 agagtggtcg aacatggggc gcaccttcat caaggcggtt ctcaattcgg ctcgcgccat
     8881 ccatcccaac gataacggcc cggcggcaca gaacgcccgt cgcatcaatg gctttgccga
     8941 tctcgacggc atcgagttct tgggcaaggt cgattgggaa aaggatcaga acggccaaga
     9001 caagtgcgtc atcaagtcag cagtgacgcc ggaccacaag gactatgccg ccttgatggg
     9061 cggggccagc caaccggccc cggcagccag tgcccccaat gcctatgctc aggccaccgg
     9121 acgctcgccg gttccgggtc gtccgagctg ggcgcagtaa gggagggcca gcgtcatgat
     9181 gctccgtccc cgtcaatcct tgctggtcga tcgcaccttg tcggcgctcg atgcctacgg
     9241 gaacacgctg gccgtcgcgc caacggggtc gggcaaaacc atcatgctgt cggcggtggc
     9301 cggcagggtg ttggtcgagc ccgatgccaa agcgtgcatc ctggcgcacc gaaccgagtt
     9361 gaccggccaa aaccgcgcca aattcgaacg cgtgaatccg ggcatgagca cgtccgtgtt
     9421 cgatgccaac gaaaagtcct ggtctggcca ggccacgttt gccatggtgc agaccttgtc
     9481 acgtctgtct caccttgaac agatgccgac actggatctg ctcgtgatcg atgaagccca
     9541 ccactcgtca tcccccagct atcgggcggt gatcgatgca gtgttggcac gcaaccccaa
     9601 ggccggcatc tgcggcctga ccgccacccc caatcgcggt gatggcaagg ggctgcgcga
     9661 tgttttctcg aacgtggccg accagatcac cctgggggag atgatcgctg ccggtcatct
     9721 ggtctcaccg cgtactttcg tcatcgatgt gggtgtccag gatgccctga aaaacgtccg
     9781 taagacggcg atggacttcg atatggatga agtcgcctcc attctcgaca agcgcttgat
     9841 cacggaagcg gtcatcaagc actggaaaga aaaggcttcg tcccgcaaaa cgatcatctt
     9901 ttgctcgacc gtcgcccatg ctcagaacgt ttgtgatgcc ttcatcgatg ccgggatcaa
     9961 ttccgtcctg attcacggtg agctgtccga tggcgatcgc aaatcacgcc tggctgaata
    10021 cgagcacggg cgtgcgcaaa tcgtggtcaa cgtggccgtg ctcaccgagg gctacgacta
    10081 cacgccgacc agttgcgttg tcctgctgcg tcccagctct tacaaatcca ccttcatcca
    10141 gatggtcgga cgcggtctgc gtaccgtcga tccggaggag ttccccggcg tcatcaagtc
    10201 ggattgcttg gtgctcgact tcggtaccgc gagcctcatg cacggttcgc tggagcaaga
    10261 ggtgaacctc gatggccatc tgcacgatgg cccggcgcca accaaggatt gtcccgactg
    10321 cggtgccgtg gtgccgctgg cctgcatgga atgtccattg tgtggctacg tctgggagcg
    10381 gcagccgcag gatctgggtg tgctggctga tttcgtcatg agcgagatcg atctgctcaa
    10441 acgttccaac ttccgctggt gcgatctgtt tggcagcgat gacgccttga tggcgaccgg
    10501 tttcaacgcc tggggtggca ttttcttcct caatggtcgt tggcatgccg tgggcggtgg
    10561 ccaaggcatg cagacacatc tgctggccgt cggcgagcgc acagtctgca tggccaaggc
    10621 cgatgactgg ctcaatgacc acgaaagtgc tgactcggcc cacaagacgc gtcgctggct
    10681 caacgagccg ccaaccccga agcaactcca gtacctgccc caagcgctgc gtgctgattt
    10741 cgggatgacc cgctaccaag cctcagcctt gctttcgttt cagttcaaca aaacgtcgat
    10801 tcagcgcctg gtcatggccg ccaacgacag ctatcgggag gcggcgtgaa atgcatcgtg
    10861 tgccaccgac aagccaaagg gtatggctgg ttcaacaccc gccgcaaacg gggcgatccc
    10921 caacgctact ccgaccagtg ggtgttctgc tcacgtcgtt gtcaggagtc gttttccaaa
    10981 ctgatgaaca agacggaggg gcaaatgatc gaccccagtg acatggaaat tgccgccatg
    11041 cgtgcttgcc tggcgccgct gggtgagtac gtcggtgaga tcggcatgca gcgtcccttg
    11101 gcggattaca gccgcgatga ggtgttgatg ctgatcgatg tcgtggtcac ggcctaccag
    11161 gactgcatgg tggccgagca cgaacgcatg gccgccaagg atcgcgcatt ctttgaggaa
    11221 cgtctggcgc gtcaggggct ag
//
LOCUS       DAOQEW010000126        11223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1756_length_11223_cov_8.468929, whole genome shotgun sequence.
ACCESSION   DAOQEW010000126 DAOQEW010000000
VERSION     DAOQEW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_11223_cov_8.468929"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..1465
                     /locus_tag="PLS35_13235"
     CDS             449..1465
                     /locus_tag="PLS35_13235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNI19680.1"
                     /translation="MSSDLHPLTLPDNGELSKTAEQPAEQATSPTPWLTILHWFDSWA
                     GIENMKTDAAPATVDWLRAIPLITVHLICLGVFIVGWSWVALGVCAAFYFIRMFAITG
                     WYHRYFSHRTFKTSRACQFAFALLGSSCAQRGPLWWAGHHRHHHIYSDKPEDTHSVRQ
                     GGFLWAHIGWISSKKFFPPRLKSIGDFAKFPELRFLDRFDTLVPTICGFGMFGLGKLL
                     ESYAPGLGTNGPQMLIWGFFVSTVALLHGTCTINSLSHVYGSQRYKTGDDSKNNFILA
                     MITLGEGWHNNHHYYAASTRQGFYWWEIDITYYMLRAMQAVGLVWDIREVPAHVREGK
                     NKLA"
     gene            complement(1513..1983)
                     /locus_tag="PLS35_13240"
     CDS             complement(1513..1983)
                     /locus_tag="PLS35_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19681.1"
                     /translation="MAQKFGNSRWVQEGFLDNRQDGTVVGRITFAVLGAIDFYLTGNC
                     RGEISGKVIRFKNSRFADEDLAGQVLGDVESPQIGDASLISFDPHPHLVPHPYIEWFS
                     MRKNHYRIELVPEDAWIATEAEIAEIDAASRDIRDRLAPQYGSKSRSADESEWV"
     gene            complement(1985..2863)
                     /locus_tag="PLS35_13245"
     CDS             complement(1985..2863)
                     /locus_tag="PLS35_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNI19682.1"
                     /translation="MPQLALLLTTFVWGATFPATKAALEQITPLAFLFLRFLLGMLLV
                     FAVLLLLRRKLTRDAAMLRMSLIASLWLFLGYVLQTVGLHLTTASNSAFITVLYVVFV
                     PLYLRRLSAHTWISNGIALIGLWFLVKPTASANLGDLLTLGSAAAFAAHMVCLERYTR
                     TTDAVSLFAWQLVMMTVAMLGAMWWEQPTAAMFEPSRVLLLGLGVTGILATGAFAVQM
                     WAQQLLPAQQVALLFAVEPAIAAWLAWYFLGENLDAQGWFGSAMILGGVLLGSRVPGE
                     MPQVSPTPVVVRSEGR"
     gene            complement(2958..3161)
                     /locus_tag="PLS35_13250"
     CDS             complement(2958..3161)
                     /locus_tag="PLS35_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19683.1"
                     /translation="MIYWIHIARAIDRVTLHRDRCSEVPSSISSSDFWEGGWFDYPDK
                     ERALAAMEQAGTTDERKCALCKP"
     gene            complement(3311..3487)
                     /locus_tag="PLS35_13255"
     CDS             complement(3311..3487)
                     /locus_tag="PLS35_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19684.1"
                     /translation="MPLLIRKYKGNGGVMQEERIDDPDRIERYMRLFEEKDVKKLETG
                     AKTVIEKDEWQLLP"
     gene            3657..3968
                     /locus_tag="PLS35_13260"
     CDS             3657..3968
                     /locus_tag="PLS35_13260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNI19685.1"
                     /translation="MPVYEYRCDQCTHRFEATQSIHARPDDTVCPQCTHMGATRILSA
                     FASKVKGSHKPGFEEIKAYDMLHERMDRFSKLPPAMGKRVDVTPDMMSGAVTPTDSPE
                     S"
     gene            4066..4890
                     /locus_tag="PLS35_13265"
     CDS             4066..4890
                     /locus_tag="PLS35_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNI19686.1"
                     /translation="MYSRISALLCAGFFALSTLPASAEVAGSMVIAGHGPEQHVMESL
                     AHAFEKANPRAYIDVVWDDNSKPLDMVKAKQAHIAVTGASANGLHSVQIAWDGIAIMV
                     HRSNFTKEVTKQEVADLFSGKYKVWADLGGPDTKVLLIDRPRNENNRDAFEQLLGITG
                     KIPDSTKVIAKDEKVIKTIAGTLPPNSAVTYLSLGQALEAVASGVPVRLLPVDKVEPE
                     TPTVKDGRYSLRRPILLISNNDADPLVAAFEQFALSDEGQKIISDSYTPLPKTSSP"
     gene            4903..5358
                     /locus_tag="PLS35_13270"
     CDS             4903..5358
                     /locus_tag="PLS35_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNI19687.1"
                     /translation="MRTFMTVALGSVVALGVLCMAVPSGQAQEGAMVEGAGYTMFNTE
                     SIKGSDTNKLEKDPVCDRSKRPSIGKVEPDEAKPGDKIVIKGENFGSKECFHGVTFSA
                     APKDKIEYQFVNESTIEAVVPDAKAGMSFIIVVAGGGSAQSKPLLIQAK"
     gene            5531..6949
                     /gene="accC"
                     /locus_tag="PLS35_13275"
     CDS             5531..6949
                     /gene="accC"
                     /locus_tag="PLS35_13275"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNI19688.1"
                     /translation="MFRKILIANRGEIAMRIIRGCRELNIATAAIYSEADSSGIYVKK
                     ADESYLVGPGPVKGFLDGKQIVEIAKRIGADAIHPGYGFLSENAKFARLCHASGITFI
                     GPSPETIDLMGSKVKARQIAQQAGLPIVPGTEGGVTSIEEALEFVHRINYPVMIKASA
                     GGGGRGLRVVRSDQELRENMEVAAREALAAFGDGSIFIEKYIERPHHIEFQILGDKHG
                     NIIHLGERDCSIQRRHQKLIEIAPSLVLTAKLRAQMGEAAIAIAKAVKYDNAGTVEFL
                     LDHEGHFYFMEMNPRLQVEHTVTEQITAIDIVRNQISIAAGKPLEIRQKDVTLQGHAI
                     QCRINAEDPRNNFMPCTGTVTAYLSPGGIGVRIDGAVYRDYTIPPYYDALLAKLTVRG
                     RTWEEAVSRMRRSLEEYVLRGVKTTIPFMKNVMMEQDFQAGRFDTSYLETHPDLYQYE
                     ESEEPEDLVLAISAAIAAYEGL"
     gene            7022..9019
                     /gene="oadA"
                     /locus_tag="PLS35_13280"
     CDS             7022..9019
                     /gene="oadA"
                     /locus_tag="PLS35_13280"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNI19689.1"
                     /translation="MAIKKTKKAVSKKSPSKKSAPAKPTKAVRPAATRTVAAAPSVSS
                     TFNVVPAPAKKLQLTEVALRDGHQCLLATRMRTEDMLPIAQKLDSVGFWSLEVWGGAT
                     FDTCLRFLKEDPWERLRALRAAMPKTKLQMLLRGQNLVGYRHYADDVLDKFIERSAAN
                     GIDVFRIFDALNDVRNLERAIREVKACEKHVEAAISYTTSPVHRLDGFVTMGKRLEDL
                     GADTICIKDMAGLLAPVDAYRLVKSLKAAVRVPIHLHSHYTSGMGTMTALMAVMAGLD
                     LLDTSISPLAGGASHPPTESMVAALRGTPYDSQLDLEDLQPIAEHFRTVRRKYRQFES
                     DFTGVDAEILTSQIPGGMLSNLAAQLAEQNALDRMKEVMDEIPRVRKDMGYPPLVTPT
                     SQIVGTQATLNVLTGEQGERYKVITTETKNYFLGLYGRAPGPLDKEIMARAIGDEEPV
                     KGRPADRLEAEFEKLKKDMPESATTLEDQLSFALFPAIARDFFEAREQGALQPEPLEP
                     TDTKGPAVAHDLHLAPAEFNITVHGENYHVVVSGSGRTTDGRKPYYIRVNDRLQEVSL
                     EPLQEVLAGVPESPEASSTSKPKRPRPTKPGDVAPPMPGRVVKVLVTDGAQVKTGDPL
                     LIIEAMKMESQVPAPMDGRVAAILVAEGDNVKIDETVIQLE"
     gene            9023..9985
                     /locus_tag="PLS35_13285"
     CDS             9023..9985
                     /locus_tag="PLS35_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI19690.1"
                     /translation="MPRPASLVRFVAPAPHHLATWFLASLLMSGCAARPDVPPWFDAI
                     QRLPVHTISVTGHRIAYLDEGEGPTLILLHGYGGSMWQWEYQQPLTKHFRVITPDLLG
                     SGLSEKPDIEYRPSALIESIRGLMDGLGLQTATVIGNSMGGGVAIGMALTYPERVDRL
                     VLIDTLPDHVRERLVSPLMQRAINTHVPAWMARFWASLFGSRTMEAVLKEVVYDHSLI
                     TPLVLERSNRNRQREDMIGPLLSLRDSLPLWEQLYAPRLTAIRQSTLILWGEHDRLFP
                     PQVGQDLQAMIPHSRLIVIPEAGHIPQWERPQAVNRHIMEFLQP"
     gene            10053..10739
                     /gene="tpx"
                     /locus_tag="PLS35_13290"
     CDS             10053..10739
                     /gene="tpx"
                     /locus_tag="PLS35_13290"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNI19691.1"
                     /translation="MRSMSAPVLSGVLVPRLLAGLLCLGLSACGTLPGTGKGGFSYKD
                     MPIADGSAVAGEGNNILFQGKPLMLTGMGIKVGDKLRDVKLAQTDLSMIPINDTKGKG
                     KVRIISIVPSLDTKVCEQQTHYLSEKNMGLDRMVELITISIDTPFAQKRFAEEAKISN
                     VTFLSDFRAADFGKAHGLLLKDLHLLSRALLVVDKDNKVRYLQITPELAQLPDLEDAF
                     RFARKLVTAS"
     gene            10770..>11223
                     /locus_tag="PLS35_13295"
     CDS             10770..>11223
                     /locus_tag="PLS35_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI19692.1"
                     /translation="MAHYDLLVIGTGPAGQKAAIQAAKLGKKAGIVERTRVVGGVCTN
                     TGTIPSKSLREAALYLSGFHQRSLYGASYRVKQDITMADLTFRSNHVINREIEIIQNQ
                     MTRNNIDLWYGTASFVDPHRVRVERADDHVEHTADFIVIACGTVPARPA"
BASE COUNT         2475 a         3470 c         3089 g         2189 t
ORIGIN      
        1 cctggcgcat gaaccagacc ggcgtacaat caaccggttc gcgccggcac gctttgagaa
       61 atcgatcatt cataatgggg cgcattttag agggccggtc cgaacagagt caaacaatct
      121 tgcgcagtgc accggcttag agccattcat ggcccgctca tcccgctccc cgggtggtac
      181 atacggctcg ggtcagggca gtagtgtggg gaaaagggtt cgtcgtgaaa ggcgaaaaaa
      241 ctcacgacat tttcctacga acctgtgtat aatggatgca cgtgtcttga gggctgagtc
      301 tatacgctca ccgatcgaca ccatccgaaa cattcagcgg cctgccccat cgcatcaatc
      361 gcaagcgccg caacgattac gtcacgctag tgcaacggcg gcgcgacccc tcggtaccgc
      421 atcctattcg cacaactgtg gaggtggcat gagtagtgat ctgcatccgc tcaccttacc
      481 cgacaacggt gagctgtcca aaactgccga acagcctgca gaacaggcca cgagcccaac
      541 gccctggctc actatcctgc attggtttga ctcctgggcc ggcatcgaaa acatgaaaac
      601 tgatgcggct cccgccactg tcgactggct gcgggccatc cccctcatca ccgttcactt
      661 gatctgcctc ggggtgttta tcgtcggctg gagctgggtt gcgctcggcg tctgcgccgc
      721 cttttacttc atccgcatgt tcgccatcac cgggtggtac caccggtact tctcccatcg
      781 gacatttaaa acgtctcgcg cgtgccaatt cgcctttgcg ttgctgggat cgtcatgcgc
      841 tcaacggggt cccctctggt gggccggcca tcatcgccac catcacatct attcggacaa
      901 acctgaggat acgcattcgg tgcgccaggg aggatttctc tgggcccaca tcggttggat
      961 cagctcgaag aaattctttc cgccccgact gaagagcatc ggcgactttg ccaagtttcc
     1021 tgaactgcgg ttcctggatc gcttcgatac cctggtgccg acgatctgcg gattcggcat
     1081 gttcggattg gggaaattgc tggagtccta tgcgccggga ctcggcacca atggtccgca
     1141 aatgctgatc tgggggttct tcgtctccac cgtcgcactg ctccatggca cctgtacgat
     1201 caactccctt tcccacgtgt atggatcaca gcgctataag acgggtgacg acagcaaaaa
     1261 caatttcatt ctcgccatga tcacgctcgg cgaagggtgg cacaacaatc accactacta
     1321 tgccgcgtca acccggcagg ggttctactg gtgggaaatc gacatcacct actacatgct
     1381 gcgcgccatg caggctgtag gtctggtatg ggatattcgc gaggtgccgg cccacgttcg
     1441 agaaggcaag aacaaactcg cctgagtcac ggcgggggcg gccaattcac tgcccgcccc
     1501 cgacactccg cgttacaccc actccgattc atccgccgac cgtgatttgc tgccatactg
     1561 cggcgccaag cgatcacgga tgtcacgact cgcagcatcg atctcagcga tctctgcctc
     1621 tgtcgcaatc caggcatcct ccggcacgag ttcgatgcga tagtgattct ttcgcatcga
     1681 aaaccattca atgtatggat ggggcaccag atggggatga ggatcgaacg agatgaggct
     1741 ggcatctccg atctgcggac tttcgacatc ccctaacact tgaccggcaa ggtcctcgtc
     1801 ggcgaaccgg ctatttttga atcggatcac cttgccggaa atttctcccc ggcaattgcc
     1861 cgtcagataa aaatcgatcg cgcccaagac cgcaaacgtg atgcgcccga ccaccgtccc
     1921 gtcctgtcga ttatcgagaa atccctcttg cacccaacgg ctgttgccga acttttgagc
     1981 catatcatct cccctctgac cgcacaacga ccggcgtcgg agatacctga ggcatctccc
     2041 ctggcacgcg agatccgagc agcacaccgc ccagaatcat cgcgctgccg aaccatcctt
     2101 gcgcgtccag attttcgccc aggaaatacc atgcaagcca agccgcgata gccggctcca
     2161 cggcaaacaa taatgcaacc tgttgtgcag gcagcagctg ctgcgcccac atctgcacgg
     2221 cgaacgcgcc ggtcgccagt atcccggtaa ctcccaggcc caacagcaac acacggctcg
     2281 gctcgaacat ggccgccgtc ggctgttccc accacatcgc ccccagcatc gctacggtca
     2341 tcatcaccag ttgccaggcg aacaacgaga cagcgtcggt cgttctggtg tagcgctcga
     2401 gacagaccat gtgtgccgca aacgccgcgg cgctgccgag cgtcagcaga tcaccaagat
     2461 tggcagaggc cgtgggcttg acgagaaacc acagcccaat caacgctatg ccgttggaga
     2521 tccaggtgtg cgcgctcaag cggcggagat acagcggaac gaacaccacg tacaatacgg
     2581 taatgaaggc cgagttggag gccgtcgtca aatgcagtcc caccgtctgc aacacatatc
     2641 cgaggaagag ccagaggctg gcgatgagac tcatccgcag catcgcggca tcgcgcgtca
     2701 gtttccggcg caataggagc agcaccgcga agaccagcag catgccgagt aaaaatcgga
     2761 ggaacagaaa cgccagcggc gtgatttgtt cgagggcggc tttggtggcc gggaatgttg
     2821 cgccccacac aaacgtggtg aggagaagcg caagttgagg catggtcagg cgattggacg
     2881 tactgaatga tgaaccggat ctgccgtgaa cgtgagccgg cgtggatcga tcgactcagc
     2941 actctcgtgt gaatcgatca gggtttgcag agcgcgcact tgcgctcgtc ggtagtaccg
     3001 gcctgctcca tcgctgcgag cgcgcgttcc ttgtcaggat agtcaaacca gccgccctcc
     3061 cagaagtcac tactcgaaat gctggagggc acttccgaac aacggtcccg atgaagtgtg
     3121 actcgatcaa tcgcgcgtgc aatgtggatc caatagatca tggtaccccg ttggactctg
     3181 tcgtcaggac cgtcgcagcc caaccctacc acgcacttct cggagagagc aatgcgcagg
     3241 acatatcaag ccgcaacgac ccgcgacgga aacacacatg agatgatggg atgaacccag
     3301 gaggtacgtg ctagggcagg agctgccact cgtccttctc gatcaccgtc ttggctccgg
     3361 tttcgagctt cttgacgtcc ttctcttcga acagacgcat ataccgttcg atccggtccg
     3421 gatcgtcgat gcgctcttcc tgcataacac cgccattgcc tttgtacttc cggattaaca
     3481 ggggcatttc aaccgctcct tcaattgtag ctcgcgtttc ggcacataga ttcggtacaa
     3541 tagtgcaaag ttatctatag ggtcaagcgt ttccaaacag agaactgctt tatctcggct
     3601 agccctccta tgggcagcca tgaagtgcat tcttctccag ctgtaaggag tcgacaatgc
     3661 cggtttacga atatcgctgt gatcaatgta cccatcggtt cgaggccacg caatccatcc
     3721 atgcgcggcc ggacgatacg gtgtgtccac agtgcaccca tatgggggca acccggatct
     3781 tgtccgcctt cgcctcgaag gtcaaaggga gccacaagcc tggatttgag gaaatcaaag
     3841 cgtacgacat gcttcatgaa cgcatggatc gcttctcaaa acttcctccc gccatgggca
     3901 aacgcgtgga tgtaacgccg gacatgatgt ccggtgcagt aaccccgacc gacagcccgg
     3961 aatcctaagc ggaggtcgcg tgagcggaag ggactgccgc cccggcctgc cgcgccacga
     4021 tgcccacgga aggccgccca ggggcgagag ggagaatcgt tcagcatgta ttcccgtatc
     4081 agcgccctcc tttgtgccgg atttttcgct ctgtcaacgt tgccggcctc agccgaagtc
     4141 gccggctcca tggtgatcgc cgggcacgga ccagagcaac acgtgatgga atccctggcg
     4201 cacgcgttcg aaaaagccaa cccccgcgcc tatatcgatg tcgtctggga cgacaactcc
     4261 aaaccactcg acatggtcaa agccaagcag gcgcacatcg ccgtcaccgg cgcctcggca
     4321 aacgggctgc actccgtcca aatcgcgtgg gacggcattg ccatcatggt ccaccgctcc
     4381 aatttcacga aagaagtcac caaacaggag gtcgccgacc tcttttccgg aaagtataag
     4441 gtctgggccg acctgggtgg accggatacc aaggtgctgc tcatcgatcg gccgcgcaac
     4501 gaaaataatc gtgacgcctt tgagcaactc ctgggcatta ccggcaagat tcccgacagc
     4561 accaaagtga tcgcgaagga cgagaaggtc atcaaaacca tcgccgggac tttgccgccc
     4621 aattctgccg tcacctatct gtctctgggc caagccttgg aagcagtcgc ctccggagtg
     4681 ccggtgcgtc tgctccccgt cgataaagtg gaaccagaaa cacccacggt caaggacgga
     4741 cgttattcct tacggcggcc gattctcctt atctccaata atgatgccga ccctctcgtg
     4801 gccgcgtttg aacaattcgc cctgtcggac gagggtcaga aaattatcag cgattcttat
     4861 acaccgctcc cgaagacatc atctccctga taggaggctt tcatgcgcac attcatgacc
     4921 gtggccctcg gttccgttgt tgctctcggc gtcctctgta tggccgttcc atcaggccag
     4981 gcccaggaag gggccatggt ggaaggcgcc ggctacacga tgttcaatac cgaatcgatc
     5041 aaggggtcgg ataccaacaa gcttgagaag gatccggtct gtgatcgctc aaagcgcccc
     5101 tccatcggca aagtagaacc agatgaagct aagcccggcg ataaaatcgt gatcaaaggc
     5161 gaaaacttcg gcagcaagga atgtttccat ggagtcacgt tcagcgcggc gcccaaagac
     5221 aagatcgagt accagttcgt gaacgaatcg acgattgaag ccgtggtgcc ggacgcgaag
     5281 gcgggcatgt cattcatcat tgtcgtcgcc ggcggcggca gcgcgcagtc gaagccactt
     5341 ctcattcagg ccaagtaatt cccgccgtgg caggaggcgc tgtgcctcct gcccgattcg
     5401 tgcccttccg gattcgccat tttaccgccc ttcgtctcga caactctctt gaccctatgc
     5461 taggttacgc gatctttttg ggaggcgacc cagaggtgag ctccggctcc gaacctcacg
     5521 caggctcagc atgttccgca agatccttat tgccaaccgt ggcgaaatcg ccatgcgcat
     5581 catccgcggc tgccgcgagc tcaacatcgc gacagcggcg atctattccg aggctgattc
     5641 ctcgggtatc tacgtcaaaa aagctgatga gtcctatctc gtaggtcctg gtccggttaa
     5701 agggtttctg gacggaaaac agatcgtgga aatcgcgaag cgtatcggcg ccgacgcgat
     5761 ccatcccggg tatgggttct tgtcggaaaa cgccaagttc gcccgcctct gccatgcctc
     5821 tggaatcacc ttcatcgggc cttctcccga aacgatcgac ctcatgggca gcaaggtcaa
     5881 ggcccggcag atcgcccagc aggcagggct tccgattgtt cctggcaccg aaggcggggt
     5941 caccagcatc gaggaggcct tagagttcgt ccatcggatc aattaccccg tcatgatcaa
     6001 ggccagcgcc ggcggcggcg gacgagggct tcgcgtagtc cgctccgacc aggagctgcg
     6061 cgaaaatatg gaagtcgcgg cacgggaagc gctggcggct ttcggcgacg gcagcatctt
     6121 cattgaaaag tacatcgagc ggccgcacca tattgaattt cagatcctgg gcgacaaaca
     6181 cggcaacatc atccacctcg gcgagcggga ttgttcgatt cagcgccggc accagaaact
     6241 gattgaaatc gccccttcgc tggtgctgac ggcaaaattg cgcgcgcaaa tgggtgaagc
     6301 cgccatcgcc atcgccaaag ccgtgaagta cgacaatgcc ggcacggtgg aattcctcct
     6361 cgaccacgaa ggccatttct atttcatgga aatgaatccc cggttgcaag tcgaacatac
     6421 cgtcacggag cagattacgg cgatcgacat cgtgcgcaat caaatttcca tcgcggcagg
     6481 caaaccattg gagatccggc aaaaggatgt caccctgcaa ggccacgcga tccaatgccg
     6541 catcaatgcc gaagacccgc gtaataactt tatgccctgc accggcaccg tcacggccta
     6601 tctttctccc ggcggcatcg gggtccgtat cgacggcgcg gtctaccggg actatacgat
     6661 tccaccctat tacgatgcct tactggcaaa actcaccgtg cgtggacgca catgggagga
     6721 agccgtcagc cgcatgcggc gttcgctcga agaatacgtc ctgcgtggcg tgaaaacgac
     6781 cattccgttc atgaagaacg taatgatgga acaggatttc caggcgggac gattcgatac
     6841 ttcctatctg gaaacccatc cggacctgta tcaatacgaa gaatccgagg agcctgagga
     6901 cctggtgttg gcgatttccg cagcaatcgc cgcctacgaa ggactctgac ctctcagcca
     6961 ccaggcacac agcacctcaa ccctgttacg cgagcgattc cgccgcctcg aggccgccag
     7021 tatggcaata aaaaagacca agaaagccgt atcgaaaaag tctccgtcca agaaatccgc
     7081 gccggccaag ccgaccaaag ctgttcggcc ggctgccaca cgcaccgtcg cagccgcacc
     7141 ctctgtatca tcgacgttca acgtcgttcc ggcaccggca aagaaactgc agctgacgga
     7201 agtcgccttg cgagacggtc accaatgtct cctcgccacc agaatgcgca ccgaagacat
     7261 gctgcccatc gcgcagaagc tggactcagt gggattctgg tctttggaag tctggggcgg
     7321 cgcaaccttc gatacctgcc tgcgttttct caaagaagat ccgtgggagc ggctccgcgc
     7381 actgcgtgcc gcgatgccga agaccaagct gcagatgctc ctgcgggggc agaatctcgt
     7441 gggctaccgg cattatgccg acgacgtgct cgacaaattc atcgaacgat cggccgccaa
     7501 tggcatcgat gtgttccgca tattcgatgc gctcaacgat gtgcgtaacc tggaacgggc
     7561 catccgcgaa gtcaaagcct gcgagaaaca cgtagaagcg gcaatttcct atacgacgag
     7621 ccccgtccat cgattggacg gattcgtcac gatggggaaa cgcctggagg acctcggcgc
     7681 cgacacgatt tgcatcaagg atatggccgg acttctggcg ccggtggatg cctaccggtt
     7741 ggtcaagagt ttgaaagcag cggttcgtgt tcccattcac ctccattccc attacacctc
     7801 gggcatgggc accatgacgg ctctgatggc cgtgatggcg ggcttggatc ttctggacac
     7861 gtccatttcg ccgctagcgg gaggggcttc gcacccgccg accgaatcga tggtcgcggc
     7921 attacgcggc accccctacg acagccaatt ggatctggaa gacctccagc ccatcgcaga
     7981 gcatttccgg acggtgcgcc gaaagtaccg gcaatttgaa agcgacttca ccggtgtcga
     8041 tgcggaaatt ctaacgtccc aaatccccgg tggcatgttg tccaacctcg ctgcgcaact
     8101 ggcggagcaa aacgccctcg accggatgaa ggaagtgatg gacgaaatcc cccgcgtccg
     8161 gaaggacatg ggctatcccc cgctcgtcac gcctaccagc caaatcgtcg gcacgcaagc
     8221 cacactcaac gtactgaccg gcgaacaggg cgagcgctac aaagtcatca ccacggaaac
     8281 caagaactac ttcctgggcc tctacggccg tgcaccaggc cctcttgata aagagattat
     8341 ggcccgagcg atcggcgacg aagaaccggt aaaaggccgg ccggcggacc gcctggaagc
     8401 agaattcgaa aaactcaaga aggacatgcc ggaatccgcc acgaccctgg aagaccagtt
     8461 gtcctttgcc ctgtttccgg ccattgcccg ggatttcttc gaggcacgcg agcaaggcgc
     8521 cctgcagccg gagccgctcg agccgaccga caccaaaggc ccggccgtgg cgcacgacct
     8581 ccatctggct ccggccgaat tcaacattac ggtgcatggc gagaattacc atgtcgtggt
     8641 ctcaggctcc ggccgcacca ccgacggccg caagccctat tacatccgcg tcaatgaccg
     8701 actgcaggaa gtctcccttg aaccgttgca ggaagtgttg gccggcgtgc cggaatctcc
     8761 cgaggccagc agcacgtcga agccgaaacg tccccgaccg acgaaacccg gtgatgtggc
     8821 cccgcccatg ccgggccgtg tggtgaaggt gttggtgacg gatggcgccc aggtgaaaac
     8881 gggcgatcct ctcttgatca tcgaggccat gaagatggaa agccaggttc cggcccccat
     8941 ggacgggcgg gttgccgcca tccttgttgc tgagggagac aacgtcaaaa tcgacgaaac
     9001 cgtcattcaa ttggagtagc cggtgccgcg cccggcctct ctcgtccggt ttgttgcccc
     9061 agcgccccat cacctggcca cctggtttct tgcgagtctc ctcatgagcg gttgcgcggc
     9121 acggccggat gtccctccct ggttcgacgc gatccagcga ttgcctgttc ataccatttc
     9181 cgtcaccggc caccggatcg cctaccttga tgaaggcgag gggccgacac tgattctgct
     9241 ccatggctac ggcggatcca tgtggcagtg ggaataccag cagcccttga ccaagcactt
     9301 ccgtgtgatt acgccggatc tcctcggctc cggcctttcc gagaaacccg acatcgaata
     9361 ccgtccctcc gcactgattg aatccatacg aggactcatg gatgggctgg gcctgcagac
     9421 agccaccgtc atcggcaatt cgatgggcgg cggcgtggcc attggcatgg ccctgacata
     9481 tccggagcgc gttgaccgtc tggtcttgat cgataccctg ccggaccatg ttcgggaacg
     9541 actcgtgagc ccgctcatgc agcgggccat caacacgcac gtgcccgcct ggatggcccg
     9601 gttctgggca tcgctgttcg gaagccggac gatggaagcc gtgctcaagg aagttgtcta
     9661 cgatcacagc ctcatcaccc cgctcgtgct ggagcgatca aaccgaaacc gccagcggga
     9721 agacatgatc ggccccttac tctcgttgcg cgacagcctg ccgctgtggg agcagctgta
     9781 tgcgccgcga ctcacagcaa ttcgccagtc cacgttgatc ctgtggggcg agcatgatcg
     9841 cctcttccct ccgcaagtcg gccaagatct ccaggcgatg attccgcact cacggttgat
     9901 tgtgattccc gaggccggcc acattcctca atgggagcgg ccacaggcgg tcaaccggca
     9961 catcatggag ttccttcagc cttgacagtc ttcccagcgg gccatacagt gacaatggag
    10021 agcatacgtc ccttttttca tggagaggtc ttatgaggtc gatgagtgcc cctgtcctgt
    10081 ctggcgttct ggttccacgt cttcttgcag gcctgttgtg tctcggcctg agcgcgtgcg
    10141 gaaccttgcc gggcactggc aagggggggt tctcatacaa agacatgccg atcgccgacg
    10201 gaagcgcagt ggccggagaa ggcaacaata tcctgtttca gggcaagccg ctgatgctca
    10261 ccggtatggg catcaaggtc ggcgacaaat tgcgcgatgt gaaattggcg caaaccgacc
    10321 tctcgatgat tcccatcaat gacaccaagg gcaaaggcaa ggtccgcatc atcagcatcg
    10381 tcccgtctct cgatacgaaa gtctgcgaac agcaaacgca ctatttgagc gagaagaaca
    10441 tgggcctcga tcgcatggtc gaactcatca cgatcagcat cgacacgccg ttcgcgcaaa
    10501 aacgattcgc agaagaagcc aagatcagca atgtcacctt tctgtccgat tttcgcgccg
    10561 ccgatttcgg caaggcccat gggcttctgc tgaaagacct tcacctcctg agtcgtgccc
    10621 tgctggttgt cgacaaagac aacaaggtgc gctatctgca gatcaccccg gaattagctc
    10681 aactgccgga cctagaggat gccttccggt tcgcgcgtaa gctggtgacg gccagctgac
    10741 tcttgtctta cggccgttga ggactatcca tggcccatta cgatctgctg gtgatcggaa
    10801 ccggcccggc aggacaaaag gcggcgattc aggccgccaa gctcggcaaa aaagccggca
    10861 tcgtcgagcg cacacgggtt gtcggcggcg tctgcaccaa caccgggacc atccccagca
    10921 aatcgctccg agaagcggcc ctctatctct ccggtttcca tcaacgtagt ctgtatggcg
    10981 ccagttaccg cgtcaaacag gacatcacga tggcggatct gaccttccgt tccaatcacg
    11041 tcatcaaccg tgaaatcgaa atcattcaga accagatgac gcggaacaat atcgacctgt
    11101 ggtacggcac cgcgtccttc gtcgatccgc atcgcgtacg cgttgagcgg gctgatgatc
    11161 acgtggaaca cacggcggat ttcatcgtca tcgcctgcgg cacggttccc gcccgtcccg
    11221 ccc
//
LOCUS       DAOQEW010000127        11049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1809_length_11049_cov_9.002001, whole genome shotgun sequence.
ACCESSION   DAOQEW010000127 DAOQEW010000000
VERSION     DAOQEW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_11049_cov_9.002001"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..304
                     /locus_tag="PLS35_13300"
     CDS             149..304
                     /locus_tag="PLS35_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19693.1"
                     /translation="MGVMPRGMTERKGDAMKQVISLAPVMLLMVLVMGAMGIMVFWGL
                     SLLDLSE"
     gene            324..1343
                     /locus_tag="PLS35_13305"
     CDS             324..1343
                     /locus_tag="PLS35_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNI19694.1"
                     /translation="MREEGDHPGRRWAIILAGGDGVRMQPVVKRWLGRTMPNHYCRYV
                     GTRSLFQHTLDRMRYVTSPARTLVVVGRGHQEAWAQIGQRHPGMVLVQPRNAGTAAGL
                     FLPLTYIRARDPAGMVVVTPANQFICPDERLPAEILYAVRAAEKLPANLVGLAARPDG
                     PDSEYAWIKSGHELVRLGLRSIRAVNTFLHRPEPDLARLAYASGALWHTGIMAASVET
                     WWNLGRRVLPEMMRLFERLGEVINTPREPQVLRDIYARMPVRSFSRDLLAAVPGVAAV
                     MEMQGVWWSDWNHPERIMKTLTRLGRDTASVMPHLRAAVAERQSAQTLDIAMAHRTDG
                     WSAEG"
     gene            1680..2660
                     /locus_tag="PLS35_13310"
     CDS             1680..2660
                     /locus_tag="PLS35_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI19695.1"
                     /translation="MTTPPFVKHILFATDFSDDASRAQEYAVHLAGACDAKLDVLHVI
                     EAPHEMQADAESLGIVGRSRDAAARQLEEVRDELARRGTPARVRLLLGNPSEHIHQAA
                     REQNIDLMVLGAQGRTNVLYGLIGKTAERLIHEGPCPVLAVPGSRQDAVEMPPTDRPA
                     LIRHILAPLDFSTPSLDAVEYAVHLAKGVGATVTLMHVLEPASYDLDCGLGVIEEESS
                     KRDYWNRQLMDLRDVVTAFDLQADVALCGGVPSDAILASALRQHADLIVMGTHGRRGI
                     SPDRCGSVAEAVLRRATCPVLTVKTGKFDPGHRRVVPNALERHVTRGENA"
     gene            2657..3070
                     /gene="rnk"
                     /locus_tag="PLS35_13315"
     CDS             2657..3070
                     /gene="rnk"
                     /locus_tag="PLS35_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HNI19696.1"
                     /translation="MNPRNICMTAVDLSRLRDVLRARTSVGRDRDHLDSLEEELDRAD
                     VVDPSAIPSNVVTMNSQVRIEDVDTGMVNVYTLVFPSDASIAERKISILAPIGTALLG
                     SRVGSTVDWPVPAGRRTMRIKDVLYQPEAAGDYHL"
     gene            3208..3642
                     /locus_tag="PLS35_13320"
     CDS             3208..3642
                     /locus_tag="PLS35_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNI19697.1"
                     /translation="MDVRSKVYTYRTAVQWTEQKKGTISSEGKPDIQVATPPEFKGHA
                     GIWSPEDLYVAAANICLMTTFLAVAERGGLAFTAYHCEAEGRLELVEGKFQVTVITLR
                     PHITLSPGGDASKAKELIEKAEANCLISNSMKTRIALEPTIV"
     gene            3856..4221
                     /locus_tag="PLS35_13325"
     CDS             3856..4221
                     /locus_tag="PLS35_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI19698.1"
                     /translation="MHLRIGVIAVSQLILLSAIVVAVYAGPGNVGKGKTLYANLCIRC
                     HGVDGKGKGEMTFTPPVADLTAPAAQTKLDATLLKTIHDGRRDTAMGAWKFVLSEEEM
                     RDVTAYLRQLGSEAGRGRP"
     gene            4241..4708
                     /locus_tag="PLS35_13330"
     CDS             4241..4708
                     /locus_tag="PLS35_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19699.1"
                     /translation="MNWRIFGWGIAALWMITIGIMSTFLVQGHTRPGDDGRTEIILAP
                     SERDLVLAEMRGLLKAIHGVVTVLGAGDQPLKAAESSARAAGMAMAADVNPAIMLKLP
                     LAFKQMGMSIHKDMDHLADAIAQGESQVHILNRLSSITARCTTCHDMYRFGTP"
     gene            4807..5025
                     /locus_tag="PLS35_13335"
     CDS             4807..5025
                     /locus_tag="PLS35_13335"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNI19700.1"
                     /translation="MACNVGGVERPVRIVLGILLIGIGVFGALPPVGMGIALAAGTIA
                     LVTGALGFCPLWSLFGINTCSVGAGKKA"
     gene            5060..5614
                     /locus_tag="PLS35_13340"
     CDS             5060..5614
                     /locus_tag="PLS35_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNI19701.1"
                     /translation="MVSHAEQPDQQPEGGFSTPVGSRRTFFQWVTLAAAGLVGVGLSI
                     PLLSTLISPAFHRRRRDWVDVGAVDDLSSGHPKQLDHVTTIRDGWLETTSHKAVWAVK
                     QAQGDIKVFSPICTHLGCGYRWDETDKKFLCPCHGSSYDVNGQVLGGPAPRPLDTLPS
                     KVEGGRLLVMYKDFKSGLPTSVEI"
     gene            5673..6998
                     /locus_tag="PLS35_13345"
     CDS             5673..6998
                     /locus_tag="PLS35_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNI19702.1"
                     /translation="MPSRLYDWLDARLNLKPVQRTLLDEPIPGGASWIYVFGSVTLFL
                     FVLQAATGMFLALYYAPTPDHAYDSVRFIETDVTLGWLVRGLHHWGASSMVVAIGLHM
                     LQTFLYGAYKAPREVMWMVGVVLFLIVMTFAFTGYLLPWDQTAYWATQIGINMVGTVP
                     LIGDVASRILRGGEILGALTLSRFFAIHVLFLPAMLITGIAVHLFVLRRVGPAGPWTD
                     DRASLGAETFAPRQVYMDAVVIAGVFLVVAALAFSIPLPLTDKANPSDTSFVPVPEWY
                     FLFYYELLKYVHGPLEPLATWILPLCVILVMLFWPFIDRNPVRNPIRRPVALGSGLLF
                     LLVVFGLLGISIRNLYAVPRVDPAVARGRAVYAQLGCVGCHRIHGEGGAVAPDLSTVG
                     DTRPDREWHLKHFRDPQSVSPGSFMPKFPLTDQQLNDLTTYMLSLKRAT"
     gene            7037..7387
                     /locus_tag="PLS35_13350"
     CDS             7037..7387
                     /locus_tag="PLS35_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI19703.1"
                     /translation="MRIWGTLLCTVGVLLGSMPISLAASGKGNPATGKKLYHESCQNC
                     HGPAGKGDSEMAAYLTPAPADLTSKTTQEKTDAQLRKVIQEGRPGTAMTGYAAAFDEE
                     KLNDVMAYIRSLKP"
     gene            7629..8474
                     /locus_tag="PLS35_13355"
     CDS             7629..8474
                     /locus_tag="PLS35_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI19704.1"
                     /translation="MRHVLFSVIVIVGLTAGCVESKDTRGAAPSAVSQSDSARLDALF
                     SHPSPDSIPGGQRGEQIRLGYELVVHTQQFAAPYVGNRLTCANCHLDAGLDPNSASFV
                     GLSRAYPEYRARAGKVLTLADRINDCFERNLNGKAIPQDSHKLQAIVAYIEWLSKDVP
                     AGSRMAWRGVPTLSSTKPPDPANGAKVFTARCAFCHGADGQGTMAAPPVWGAQSYTIG
                     SDMARVSVAASFIKQNMPRTRGWALSDQEAYDVAAYINSQPRPDVAGRGRDWPKGEKP
                     ADAPN"
     gene            8558..8956
                     /locus_tag="PLS35_13360"
     CDS             8558..8956
                     /locus_tag="PLS35_13360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNI19705.1"
                     /translation="MKLSVAYQGGTRYDILSDRHRVVTDQPTEDGGTDAGMSPVELFV
                     GSVASCVGYFVGQFCARHDISREGLKVDAEWAVAEGPHRVGQIQLAIRLPHRVTPEQK
                     ERLLRVAHACTVHQSIMVPVQVAIELNPHT"
     gene            8988..10169
                     /locus_tag="PLS35_13365"
     CDS             8988..10169
                     /locus_tag="PLS35_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HNI19706.1"
                     /translation="MAVTRRALFERVLQGLGAAVVAGGVDRAFADSQSDVIGQVSGPL
                     TVRVSRPFDAETPVREFGSWLTPNERFFVRSHFGPPGAETIQPEAWRLTVKGLVQNEL
                     TLTLSELRKFEQVTITAVLQCSGNGRAHHRPKVPGVQWERGAAGNAQWTGMRLRDLLQ
                     RAGVKPQGLHVQMQGADRPALPTVPLFTRSIPIAKAFHPDTLLAYEMNGRPLPLLHGA
                     PLRVITPGWMAESCMKWLTELTLRADETPGYYMQQAYRIPETSIQPGSGLPGSVMVPV
                     EQMPVKSLIAAPGEGDTLKAGPVTIQGVAWAGESPITKVEVSCDDGKTWEPARLLGEA
                     QPYAWRQWQYLWNARQVGPTTILCRATDAQGAQQPEKSPWNPGGFLWNGWDRLAVTVA
                     A"
     gene            10166..10813
                     /locus_tag="PLS35_13370"
     CDS             10166..10813
                     /locus_tag="PLS35_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI19707.1"
                     /translation="MRGVMEGAQRERGRWSLLAAGLVLLLGIAAGTAAQEDDKHTIEP
                     AQARRAVTLIHARCAVCHTTDLIVQQRLPPDRWQITVEKMMHWGADLSKDEAAALLQF
                     VTARYHPGAPDHLPSIEEELALTAPAGGPSTASDGPLVGVPARGAEIFARNCQACHGE
                     GAMGGAGPKLARNKILKNEGVFWETVLHGRGPMPAWGSVLSHQEVADIHAWLASR"
     gene            10850..>11049
                     /locus_tag="PLS35_13375"
     CDS             10850..>11049
                     /locus_tag="PLS35_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19708.1"
                     /translation="MGGRRTAMIGCALVILVGLFSFPRLLLGSDQTRVKEMIQNNCAG
                     CHRLEGKAESRFNLKAPDLIWAG"
BASE COUNT         2150 a         3011 c         3610 g         2278 t
ORIGIN      
        1 cgtccggcag ctcgtcgttc ttcctagtcg tgcctatcct cttttcgttg tcattccttc
       61 cagccgcatg tcgtcagcat gaagaattct tcatgcccta ctcatcggca cttcatctcg
      121 cgccggtaga gtgtggtgac ggaatggcat gggtgtcatg ccgcgcggca tgacagaacg
      181 aaagggcgat gcgatgaagc aggtcatttc cttggcgccg gtgatgttgc tcatggtctt
      241 ggtgatgggc gcaatgggaa tcatggtctt ctggggcctg tccctgctcg acttatccga
      301 gtaagacacc gggaggaggc gtcgtgagag aagaaggaga tcatcccgga cggcggtggg
      361 cgatcatcct cgcgggggga gacggagtcc gcatgcagcc ggtcgtgaag cgctggttgg
      421 gtcggacgat gccgaatcac tactgtcgat atgtcggaac caggtctctg tttcaacata
      481 cgctcgaccg catgcgctac gtcacgagtc ctgcgcgcac gctcgtggtg gtcggacgag
      541 gtcaccaaga agcctgggcg cagatcggtc agcgacatcc tggcatggtc ctggtgcaac
      601 cccgcaacgc cggcacggca gccggattgt ttctgcccct gacctatatt cgtgcgcggg
      661 atccggcggg gatggtggtg gttacgccgg ccaatcaatt catctgcccg gacgagcggt
      721 tgcccgcgga gattttgtac gccgtccggg cggcggaaaa gttgccggcg aacctggtcg
      781 ggcttgccgc gcgaccggat ggaccggaca gcgagtatgc ctggatcaaa tccggtcatg
      841 aactggtcag gctgggcctt cgctcgatcc gcgcggtcaa caccttcttg cacaggccgg
      901 agcccgacct ggcgcggttg gcctatgctt cgggagcgct gtggcataca gggatcatgg
      961 cagcgagtgt tgagacgtgg tggaatctcg gccggagagt cctgccggag atgatgagat
     1021 tgttcgagcg actgggcgag gtgatcaata ccccgaggga accacaagtc cttcgcgaca
     1081 tttacgcgcg catgccggtc cgaagtttct cacgggatct cctggctgct gtaccaggcg
     1141 tagccgcagt gatggagatg cagggagtat ggtggagcga ttggaatcat ccggagcgca
     1201 tcatgaagac actcacgcgt ctgggacggg acacagcctc ggtcatgccg cacctcagag
     1261 ctgcagtggc ggagcggcaa tccgcgcaga cgctggatat cgctatggcg caccgtactg
     1321 atggatggtc agcagaggga tgacagatgg cggccatgtg aacgtatggc cgatcgattg
     1381 gcgctgctcc gggcacagcg gggtgtatcg ccccaccggt gggtagcggc tgccgcaaat
     1441 gtcaacaatg cgtgtggatc gcggcagggg cactagccgg aaatggaggc tcgaccgcgg
     1501 acttccgggc ggaggtgaat gaacaagacg tggcacttgt cttgcgtcaa cgacacctgg
     1561 gttcctcacg ttcatctctc catggcatga gtcccgcgct ccggttcagt tctcggccgg
     1621 cgctctccga tggacctccg gccgcccgga acaatcgtcc aacttgtgaa ggagaagcca
     1681 tgacgacacc accattcgtg aagcacatct tattcgccac ggatttttca gacgatgcca
     1741 gccgtgcaca agagtatgcg gttcatctcg ccggcgcctg cgatgcgaag ctggatgtct
     1801 tgcatgtcat cgaggcgccc catgagatgc aggcggatgc ggaatctcta ggcatagtgg
     1861 gtcgctcgcg ggacgcggct gcgcgacaat tggaagaggt gcgggacgag ttagcgcgcc
     1921 gaggcactcc agccagagtc aggctgctac tgggaaatcc cagcgagcat attcatcagg
     1981 ccgccagaga acagaatatc gatctgatgg tgttgggcgc gcagggacgc accaatgtgc
     2041 tctatgggct gatcgggaag acggcggaac gattgattca tgaggggccc tgtccggtgc
     2101 tggccgtgcc tggatcgagg caggatgccg tggaaatgcc gccgaccgat cggcctgcgc
     2161 tgattcggca tattctggcg ccgttggatt tttcgacgcc atcgctggat gcggtggaat
     2221 atgctgttca cctggccaag ggggtcggcg ctaccgtgac gctgatgcac gtgctggagc
     2281 cggcctcgta tgatctggac tgtggtctcg gtgtgattga agaggaatcg tcgaagcgag
     2341 actattggaa tcggcagttg atggacctgc gagacgtggt gacggctttc gaccttcaag
     2401 ctgacgtggc cctctgcggc ggcgtgcctt ccgatgccat cctggcctcc gccttgcggc
     2461 aacatgccga cctcattgtg atggggacgc atgggcggag agggatttct ccggaccgat
     2521 gtggaagcgt cgcagaggca gtgttgcgcc gggcgacctg cccggtgttg accgtaaaaa
     2581 cgggaaaatt cgatccaggc catcgccggg tggtgccgaa tgcgctggag cgacatgtca
     2641 ccagaggaga gaacgcatga atccccgtaa tatctgcatg actgccgtgg acctgtctcg
     2701 cctcagagac gtgctgaggg cccgcacgag cgtcggccgt gatcgggacc atctggatag
     2761 tttggaagag gagctcgatc gcgctgatgt cgtggacccc tccgccattc cgtctaacgt
     2821 ggtcacgatg aactcacaag tccggatcga agatgtggat accggcatgg taaatgtcta
     2881 tacgctggtg tttccttcgg acgccagcat cgctgaaagg aaaatatcga tcctcgcgcc
     2941 gatcggtacg gcgttgttgg gttcccgggt gggctcgacg gtcgattggc ccgtcccggc
     3001 gggaagacga accatgcgca tcaaggacgt tctgtaccaa cctgaggccg caggcgatta
     3061 ccacctgtag gtatgtggtg gtgaaggcat cagagttgct catcccatag agtgtcactg
     3121 aagaagagcg gatgttcgtc gtgcacgaga cgaggcatgt cgccgtaccc ttttcaatca
     3181 tgcgcccagc acagttcgga ggtatcaatg gacgtacgca gcaaggtgta tacgtatcga
     3241 accgcggttc agtggacgga acaaaagaaa gggacgattt caagtgaggg taagccggac
     3301 attcaggtgg caacaccgcc tgaattcaag gggcatgcag gaatctggtc accggaggat
     3361 ttgtatgtgg cggcggcgaa catctgcctc atgaccacat ttctggctgt tgcggaacgg
     3421 ggcggcctgg cgttcaccgc gtaccactgt gaagcggaag gtcgtctgga actcgtggag
     3481 ggaaagtttc aggtcacggt gatcaccctg cgcccacaca tcacgctttc gccaggaggg
     3541 gatgcttcga aagcgaagga actgattgaa aaggcggaag ccaattgcct gatctccaac
     3601 tcgatgaaga cccgtatcgc gttggagccc acgatcgtct gacgtgccgg ctgtggcatc
     3661 ttgcgccagg atcaggtgtg ggctggggtg attctctcca gtccaccgtg ctgcgttctt
     3721 ccccgcctct ataaacgatc tctatccaga gcattgaccg agatcctcgt gccggttcga
     3781 ttggcacggg gatcgcatct actcctgtgt ggcgcttggg tctttccgat ggggccgctc
     3841 aaaaaggggc acactatgca cctacgaatc ggagtgattg ctgtctcgca gctgatcctc
     3901 ttgtcggcga ttgtggttgc cgtgtatgcg ggccccggga acgtcggcaa gggaaagacg
     3961 ctttacgcca atctgtgcat ccgttgccat ggtgtggacg gaaaaggaaa gggcgagatg
     4021 accttcacgc cgccggtcgc cgacctcacc gctcctgccg cgcaaaccaa gttggatgcc
     4081 acgctactaa aaacgattca tgatgggcga agggacacgg cgatgggggc ctggaagttt
     4141 gtcctatcgg aagaagaaat gcgtgatgtg acggcctatc tgcggcaatt gggcagtgag
     4201 gctggccggg gacgtcccta gaaatggaac ctctcatctg atgaattggc gcatctttgg
     4261 ctggggaatt gcggcactgt ggatgatcac gatcggcatc atgtcgacgt ttctcgtgca
     4321 aggtcatacg agaccggggg atgacggccg cacagaaatc atcctggcgc catccgagcg
     4381 agatctggtg ctggcggaga tgcgcggctt gctcaaggcg atccatgggg tggtcaccgt
     4441 gctgggagca ggggatcaac cgttgaaggc ggctgaaagc tccgcccgtg cggccggcat
     4501 ggcgatggca gcggacgtca atccggccat catgttgaaa ctgcccctag cctttaaaca
     4561 gatgggtatg tcgattcaca aagatatgga ccaccttgcc gacgccattg cacaaggtga
     4621 atctcaggtc cacattctca atcgcctctc gagcatcaca gctcgctgca ccacctgcca
     4681 cgacatgtat cgctttggga caccataagc cgcgacgaat cctttttgcg cggcgatact
     4741 ctacttgaat agaagcgggt gaacggtttt gatacgcagt cgatgcttca aacaagggag
     4801 gccatcatgg catgcaatgt gggtggagtt gagcgaccgg tccgaattgt gctgggcatt
     4861 ctgttgatcg gcatcggggt atttggggcg cttccgccgg tgggaatggg gatcgccttg
     4921 gcggcgggaa cgattgcgct ggtgacggga gccttagggt tctgtccgct ctggagcctg
     4981 tttggcatca acacctgttc ggtcggagct ggcaagaaag cctgatcggt gccgaagacg
     5041 gggcgggcga gggcaagcga tggtgagcca tgcagaacag cctgatcagc agccagaagg
     5101 cggattttcc acgccggtcg gttcgcgacg gacgtttttt cagtgggtta cgctagccgc
     5161 ggccgggctg gttggtgtcg ggctgtccat tccgttgctc agcacgctga tctcgccggc
     5221 ctttcatcgg cgccgtcgcg actgggttga tgtcggcgct gtggatgact tgtcttccgg
     5281 tcaccccaag caattggacc atgtgaccac gattcgggac ggctggttgg agacgacctc
     5341 acacaaggcg gtgtgggcgg tcaagcaggc gcagggcgac atcaaagtgt tctcgccgat
     5401 ctgcacgcat ttggggtgcg gctaccgctg ggacgaaacg gacaaaaaat ttctctgtcc
     5461 ctgtcatggg agttcgtatg acgtgaacgg ccaggtattg ggagggccag ctccgcggcc
     5521 actcgatacg cttccttcga aagtcgaagg agggcggctt ctcgtcatgt acaaggactt
     5581 caagtcgggt ctgccgacca gtgtcgagat atgaacaact ggtaagtggc gccatcgaca
     5641 ggtttacctc gcgcctcaca actgggagtt gaatgccttc tcgtttgtac gattggctcg
     5701 atgctcgcct gaatctgaaa ccggttcagc ggacgttgct cgatgagccg ataccgggcg
     5761 gagcgagttg gatttatgtc ttcggttcgg tgacgctgtt cctgttcgtt ctccaggccg
     5821 ccacgggcat gttcctcgcg ttgtattacg cgcccacccc agaccatgcc tacgacagtg
     5881 tccgcttcat cgaaaccgac gtgactctcg gctggttggt gcgaggcctt caccattggg
     5941 gcgcatcgtc gatggtcgtg gccatcgggc ttcacatgct ccagacgttt ctgtatggcg
     6001 cgtataaagc accgcgtgaa gtgatgtgga tggtaggggt ggtcctgttc ctcatcgtga
     6061 tgaccttcgc cttcaccggt tatcttctcc cgtgggatca aacggcttac tgggcgaccc
     6121 agatcgggat caatatggtg ggcaccgttc cgctgatcgg cgatgtagcc tctcgcatcc
     6181 tgcgcggcgg ggagattctg ggggcgctga cgctctcacg attcttcgcc attcatgtgc
     6241 tgtttctgcc cgcgatgctc atcacgggaa ttgcggtgca cctcttcgtc ctgcggcgag
     6301 tcggacccgc cggaccctgg accgatgatc gggcctcact gggtgctgaa acctttgctc
     6361 cgcggcaggt gtacatggac gccgtcgtca tcgccggcgt gttcctggtt gtcgcggcat
     6421 tggcattcag tattccgctg cccctgaccg acaaggcgaa cccgtcggat acgagttttg
     6481 tgccggtgcc tgaatggtac tttctctttt attatgaatt gctcaagtat gtgcacgggc
     6541 cgctggaacc gttggcgacc tggattctgc cgctctgtgt gatcctggtc atgctgttct
     6601 ggccgtttat cgaccgcaat ccggtacgaa atcctatccg tcgtccggtg gcgctcggga
     6661 gcggcctgct gttccttctc gtcgtgttcg gcctgctagg catttcgatc aggaatctct
     6721 atgcggtacc aagggtagat ccggctgtgg cgcgggggcg tgccgtctat gcgcagctcg
     6781 gttgcgtcgg gtgccatcgg attcacggcg aaggaggggc ggtggcgccg gacctgtcga
     6841 cggttggaga cacgcggcct gatcgggagt ggcacttgaa gcatttccgg gaccctcaat
     6901 ccgtatcgcc gggctcgttt atgccgaaat tcccgctcac cgatcagcag ttgaatgact
     6961 tgaccacgta tatgctgagt ttaaaacggg ccacctgatg aggaaattcc tcacaaccag
     7021 aagggagaga atgggtatgc gaatctgggg cacgttgctg tgcactgtgg gggtgctgct
     7081 gggatcgatg ccgataagtt tggctgcgtc cggtaaagga aatccggcca cggggaaaaa
     7141 gctctatcac gagagttgcc agaactgcca tgggcctgcc ggaaaaggcg atagcgagat
     7201 ggcagcctac ctcacgccgg caccggccga cctcacctcc aaaaccactc aagagaaaac
     7261 ggatgcgcaa ttgcgcaagg tgattcagga agggcgtccc ggcaccgcca tgaccgggta
     7321 tgccgcagcc ttcgatgaag aaaaattgaa tgacgtgatg gcgtacattc gttcgctcaa
     7381 gccgtgaggg aaccaggatg acgccgatgc atgctttccc gaatcctttt cctcacatct
     7441 gtccctaacc tgcatcttga atgtgcgggc tccagagtgc tgtagcacga tgccgcaggc
     7501 agcagaggag gactgtggct tttatcgcca aggttatcag ggagcatccc gacgcatgcc
     7561 cgtcaaaagc caattctttt tgacggcctc ctgtcgacgt ggtatgtagt ttgcgagact
     7621 ctctacgcat gcgacatgtt ttgttcagcg tcatcgtgat cgtcgggttg acggcaggct
     7681 gtgtcgagtc gaaggacaca cgcggagccg caccatcggc cgtgagtcaa tctgactctg
     7741 ccaggctcga cgcgctcttc agccatccct cgcccgattc cattcccggt gggcagcgcg
     7801 gggaacagat tcgcctcgga tatgaactcg tcgtccatac gcaacaattc gctgctccct
     7861 acgtcggcaa ccggctgacc tgcgccaatt gccacttgga tgcgggactc gatcccaatt
     7921 cggcctcctt tgtggggctc tcgcgggcct atccggaata ccgggcccgg gcggggaaag
     7981 ttctgacgct ggccgaccgt atcaacgact gtttcgagcg taatttgaac gggaaggcga
     8041 ttccgcaaga cagtcacaag cttcaagcga tcgtcgcgta catcgaatgg ctgtcgaagg
     8101 atgtgccggc tggtagccgc atggcctggc gcggagttcc gactttgtca tcgaccaagc
     8161 ctcccgaccc tgcgaacggc gcgaaagtgt tcaccgcgcg ttgcgcattc tgtcacggcg
     8221 cggatggaca aggcaccatg gcagcccctc cggtctgggg cgcgcagtcc tacactatcg
     8281 gctcggatat ggctcgtgtg tcggtagccg cctcgttcat caaacagaat atgccacgta
     8341 ctcgagggtg ggcgctgtcg gatcaggaag cctacgacgt cgcagcttac atcaattcgc
     8401 aaccgcgacc ggatgtggca gggaggggcc gtgattggcc gaagggagag aagccggcgg
     8461 atgcgcccaa ctgaacgcgt accatgccac tggtgataac ggccggtgag gaacgcacga
     8521 ccatgcggac tgcgggatgc ggacggagga gaacagaatg aagctcagcg tggcgtacca
     8581 gggaggaacc cgctacgaca ttctcagcga ccgccatcgc gtcgtaacgg atcagcccac
     8641 cgaagacggc ggtaccgatg cgggcatgag cccggtggag ctgtttgtcg gctccgtcgc
     8701 gagctgcgtg gggtattttg tcggccaatt ctgcgcacgg cacgacatct ctcgtgaagg
     8761 tctgaaggtg gatgccgaat gggccgtggc ggaagggccg catcgtgtcg gacagattca
     8821 gttggcgatt cgtctccccc accgagtgac ccctgaacag aaagagcggc tgttgagggt
     8881 ggcccatgcg tgcacggtac atcaatcgat catggtcccg gttcaggtag cgattgagtt
     8941 gaatcctcat acgtagaagg tgaggtgggc atgaggaaag ggcgaatatg gcagtgacga
     9001 ggcgggcgtt gttcgaacgg gtgctgcagg gcctcggcgc ggctgtggtc gcgggcggcg
     9061 tcgatagagc gttcgccgac agtcagagcg acgtcatcgg gcaggtgagc ggcccgctca
     9121 cggttcgggt ctcccggccg ttcgatgcgg aaacacccgt gcgggaattt gggtcctggc
     9181 tcacaccgaa cgaacgattt tttgtgcgca gtcacttcgg gccacccggt gcggagacaa
     9241 ttcaaccgga ggcatggcgg ctgacggtaa aaggactcgt acagaatgag ttgaccctga
     9301 ccctgtccga actcagaaag ttcgagcaag ttaccattac cgccgtgctg caatgcagtg
     9361 gcaacggtcg tgcgcaccat cgccccaagg ttcccggcgt gcaatgggaa cggggcgcag
     9421 cgggaaacgc gcaatggacc ggtatgcggc tgcgcgacct cttacagcgt gccggggtaa
     9481 agccgcaggg cctccatgtg caaatgcaag gagccgatcg tccggctctg cctaccgtgc
     9541 ctctcttcac gcgaagcatt cccatcgcca aagcgtttca tccggacacg ctcctcgcct
     9601 atgagatgaa tggtcggccg ttaccgcttc tgcatggcgc accgctgcgg gtgattacgc
     9661 cgggctggat ggccgagtcc tgcatgaaat ggttaacgga gctcaccctg cgtgcggacg
     9721 aaacacccgg atattacatg cagcaggcct atcgtattcc ggagacgtcg atccagccgg
     9781 ggtccggttt gccgggaagc gtgatggtgc cggtcgagca gatgcccgtg aagtcgctca
     9841 ttgccgcgcc cggcgaaggg gacacgctga aagccggtcc ggtgaccatt caaggggtgg
     9901 cctgggcggg tgaatcgccg atcaccaagg tggaagtatc ttgcgacgac ggaaagacct
     9961 gggaaccggc tcgtttgctg ggtgaagcac aaccctatgc ctggcggcaa tggcaatact
    10021 tgtggaatgc gcgtcaggtg gggccaacca cgatcctctg ccgggcgacg gatgcgcagg
    10081 gggctcagca accagagaag agcccctgga atccaggggg gtttttgtgg aatgggtggg
    10141 atcgcctggc tgtgacggtg gcggcatgag gggcgtgatg gagggggcgc aaagggagcg
    10201 agggcgatgg tctttgttgg ctgcgggact ggtactgctc ctcggcattg ctgccgggac
    10261 ggcggcgcaa gaggatgaca agcatacgat tgaaccggcg caggctcgcc gggcggtgac
    10321 gttaattcat gcccgttgcg ccgtgtgcca cacgaccgat ctgatcgtgc aacagaggct
    10381 accgccggat cgatggcaga tcacggtgga gaagatgatg cattggggcg cggacttgtc
    10441 taaggacgaa gccgcggccc ttcttcagtt tgtgacggcc cgctaccacc ccggtgcgcc
    10501 ggaccacttg ccgtcgatcg aagaagaatt ggcgctgacc gctccggctg gagggcccag
    10561 caccgcgagt gacggcccgc ttgtcggggt gccggcccga ggcgcggaaa tctttgcgcg
    10621 gaactgccag gcgtgccatg gcgagggggc gatgggaggc gcgggaccca aactggcccg
    10681 caataaaatc ttgaaaaatg agggagtctt ctgggagacg gtgctccatg gccggggccc
    10741 gatgccggcc tggggctcgg tcttgagtca tcaggaggtc gcagatatcc atgcctggtt
    10801 ggcatcacgc tgaccgggtg cgcagtgagg tgaacgaaag gggtgtttca tgggtggaag
    10861 acggacggcg atgatcgggt gcgcgttggt catattggtg ggactgtttt cattcccccg
    10921 cctgctcttg ggcagtgatc agactcgcgt caaggagatg atccagaata actgtgccgg
    10981 atgtcaccgc ctggaaggga aagcggagtc gcggttcaac ttgaaggcac ccgatctgat
    11041 ttgggccgg
//
LOCUS       DAOQEW010000128        10968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1837_length_10968_cov_7.583066, whole genome shotgun sequence.
ACCESSION   DAOQEW010000128 DAOQEW010000000
VERSION     DAOQEW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_10968_cov_7.583066"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2804
                     /locus_tag="PLS35_13380"
     CDS             <1..2804
                     /locus_tag="PLS35_13380"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNI19709.1"
                     /translation="ITWDAVPDAMYYNLYFMTTKGVQIKFSELTRPIASAEDFKTVIG
                     VTKEKGTCIEGAQSPFMHDDLANGTCYHYVVTVVTQKGESPESQEVMAIPAPYLIAKV
                     IGQEGVDEGEFSSPTGIAVDKDGNLYVADTDNHSIQKFDKEGKFLGRWGGEAGSAEGN
                     YYYPRGLTTNNDGQVYVADTGNNRVQRLDTDGNPMKSWGKFGFAWRGADMNKFDAPWG
                     VTTDQEGNIYVTDTNNARIQKFKGDGTPLLKWGRDGSFDGAFFFPRGVAVDFVGNTYV
                     ADEGNNRIQKFDTRGSFLTKWGREGSGPGQFKAPWGVTCDALGNVYVVDQGNHRIQKF
                     DGNGTFLCAWGNRGKTEGQLNFPSGVAVDKEGAVYVVDSGNHRVIKYVPTDEELNRGK
                     EERERAQVVSEYPAPGNLAIKPGDTETFLSWMEVPKATSYNLYFHTSPNLTVEGGTKI
                     EGVTSPYNHTGLTNDQAYFYALTAVYEDGTESTLSEEVSATPVLIDITAPQTPYAVIN
                     HGAFMTNSPEIVVTISATDLDTGVAAYYISENPMTPMAGTPGWVDVPPAIKFGATIPF
                     ILSPGDGQKTVIVWFKDLGNNISTPASATILVNTSGYLCVSKWGKPGRGASLLHGGEF
                     MAPMYGLAIDQQGSIFVVDNGNNRIQKFDRNGNFIILWGNFGAANANFHNPTGIACDA
                     KGDVYVVDTNNHRVQKFDGKLGGYMMKFGSRGNGEGQFNAPWGIAIDRVRGYIYVVDS
                     ANFRVQKFDMTGEFIMSWGSFGNGDGQFYFPRGIAVDQADGTVYVVDMGNHRVQKFDT
                     STNVLPQLLTKWGGSSEAGHASSPLAQEAGQLRSPWGIAVDGQGDVYVTDTGNHRLEK
                     FDREGNFISQWGGFGGGDGQFNFPYGIVVDSRGSVFAVDSGNTRVQQFMPAEEGSERL
                     QEEADAAAELGQIDKTTRV"
     gene            2843..4309
                     /gene="guaB"
                     /locus_tag="PLS35_13385"
     CDS             2843..4309
                     /gene="guaB"
                     /locus_tag="PLS35_13385"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNI19710.1"
                     /translation="MLEKEIRLGLTYDDVVLVPAKSQVLPTEVDTRTRLSRNIHLNIP
                     IVSAAMDTVTEARLAIAMAQEGGIGIVHRVLSPADQAAEIDRVKKSESGMILDPITIS
                     PDQTIRDAHDLMARYRISGIPVTKGGKLVGILTNRDLRFETRMDLKVSQVMKRDKLVT
                     APEGTSLEKAREILHEHRIEKLPVVNKQFELKGLITIKDIEKRIKYPNACKDAHGRLR
                     VGAALGVGPDTKDRVDLLVKAGVDVVVVDTAHGHSQAVLDTVKMVRKAHPKLDIIAGN
                     IATAQAAKDLVKAGVDAVKVGVGPGSICTTRMVSGAGMPQLTAIADCARALAGSGIPV
                     IADGGIKYSGDITKALAAGASVVMLGSLLAGTEEAPGETVLFQARTYKVYRGMGSIGA
                     MERGGGDRYGQGGRPTPKLVPEGIEGRVPYKGLLAPHIYQMVGGVKSGMGYCGCKTIP
                     DLQQKATFIRQTLAGLREGHVHDVIITKEAPNYRTDWE"
     gene            4397..5953
                     /gene="guaA"
                     /locus_tag="PLS35_13390"
     CDS             4397..5953
                     /gene="guaA"
                     /locus_tag="PLS35_13390"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNI19711.1"
                     /translation="MELWHDRILVLDFGSQYTQLIARRIREAQVYSQILPCTVSLATV
                     LAYRPKGIVLSGGPSSVYDKKAPKIPKQLFDQGIPILGICYGMQLVTHLQGGEVEKAP
                     HREFGRAELQLDDRSDLFKGIGRGGSTVVWMSHGDRIERMPPGFRSIAHTDNSPIAAM
                     KRHDAKQRIYCLQFHPEVAHTAEGATILKNFVYDICGCKPTWTMRSYVDTAVEQIRQQ
                     VGTERVICALSGGVDSSVAAALTHRAVGDQLTCIFVDNGVLRAGEREQVEKTFASQLH
                     LNMRILDRTTQFMTALKNVTDPERKRKIIGRLFIKNFEAESKKLKGIKYLVQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPTKMKLKLVEPLRELFKDEVRVLGQELGLPDEIIW
                     RQPFPGPGLAIRVLGAVSKERLAILRGAETIVDQEIRAAGLYREIWQAFAVLLPIRTV
                     GVMGDQRTYEYVIAIRAVTSLDGMTADWAKIPNDVLGRMSSRIINEVKGVNRVVYDIS
                     SKPPSTIEWE"
     gene            6028..7284
                     /locus_tag="PLS35_13395"
     CDS             6028..7284
                     /locus_tag="PLS35_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNI19712.1"
                     /translation="MTVRLQKLIAGTGIASRRKAEELIVSGRVTINGHVVKELGTKVD
                     PERDHVKVDGKHLRAAQPYVYLILNKPKNVMSTLDDPEGRPTVKDFLRGVTVRVFPVG
                     RLDFDSEGLMLLTNHGDLAQALLHPRYHVPKTYLIKVKGVLDDTKIETLERGVKLEDG
                     FTAPAKINKVSRAESNSWLEVTIHEGRKHQVKRMIEAVGHSVIKLTRVRMGPLLLGDL
                     ASGEYRFLTDREANRLRELVEDRVARAEHPELDASGKPVRWMPPTEPAPPRPPKPERS
                     GRGPKSQRPDFGPRSVRGSGGANSGRPGRGALKRPLSPGAKFRRPAKTTGFQRPRPGA
                     VPQGDDRVPMANPPDSVRKPRPSGAGFQRGSFDRKSQGAGRKTPGNHSGSDQKFQQNR
                     RSGPSGSRPGQRGPARQSRNSTRRRA"
     gene            7281..9044
                     /gene="aspS"
                     /locus_tag="PLS35_13400"
     CDS             7281..9044
                     /gene="aspS"
                     /locus_tag="PLS35_13400"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNI19713.1"
                     /translation="MKIRTHRCGELTKAHVGQQVVLNGWVQRRRDHGMVLFIDLRDRT
                     GLTQVVFNAERNAAVHQAAHTLRSECVVSVTGQVMARPEESRNPHLPTGEIEIFVDAV
                     EILNEAKTPPFMIEDDIEITESLRLKYRYLDLRRPRMQRLLGLRHGIMQAVRAFLNAE
                     HFLEVETPVLTKSTPEGARDYLVPSRVNPGMFYALPQSPQLFKQVLMVSGVDRYYQIA
                     RCFRDEDLRNDRQPEFTQIDLEMSFVDREQVMSLMERMIVSVFKDAGGVQLPVPFPRM
                     TYAEAMGRYGSDKPDLRFDMPLYDVTAFAAKSDFKVFKDAATKGGLVKALIVSGGATI
                     ARSRIDALGETAKSFGAKGLAWLKVTAEGQLESVIAKFLDAQAFAAALPEAKPGDLVL
                     FGADKPAIVHDVLGRIRLLLGEELNLIDKTAWKPLWVTEFPLLDYSPEEKRYIFMHNP
                     FAAPMDEDIPFLDTEPLKARAKAYDMVLNGSEIGGGSIRNHRSDIQFRILDLLGIGKD
                     QAQAKFGFLLEALEYGAPPHGGIAFGLDRLIMLLGGADSIRDVIAFPKTQRAQCPLTE
                     APSAVSTDQLLELRIKLDLVE"
     gene            9062..10225
                     /gene="aroC"
                     /locus_tag="PLS35_13405"
     CDS             9062..10225
                     /gene="aroC"
                     /locus_tag="PLS35_13405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNI19714.1"
                     /translation="MAGNTLGRVFTVTSFGESHGPAIGCVVDGCPPGLALSAEDIQQD
                     LDRRKPGTSRHVTQRQESDHVEILSGVFEGRTTGTPIALLIRNEDQRSRDYGNLIDTF
                     RPGHADYTYWQKYGIRDHRGGGRSSARETAVRVAAAAIARKWLYETHGVMIRGYLSQL
                     GPIEAPFQSWDTVSRNPFFSANAEVVERLDSFMDELRKAGDSVGAKITTVAEHVPVGW
                     GAPVYAKLDADLAGAMMSINAVKAVEIGAGFASVAQRGSEHGDELTPEGFVTNHAGGI
                     LGGISTGQDVVVTIAIKPTSSIRVPRRSIDKTGKPQSVETNGRHDPCVGIRATPIAEA
                     MMALVLMDHALLHRAQNADVKTATPKIAGSINRLGSSASSKASSKPNAGPTEV"
     gene            10352..>10968
                     /gene="rnhA"
                     /locus_tag="PLS35_13410"
     CDS             10352..>10968
                     /gene="rnhA"
                     /locus_tag="PLS35_13410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNI19715.1"
                     /translation="MIEIYTDGACSGNPGPGGWGVLLRLDGEETELCGGEPATTNNRM
                     ELLAVIEALQWFTHPVKARVYTDSQYVQKGISEWIHSWKRRGWKTASKEPVKNEDLWR
                     RLDALASGHTIEWHWVRGHNGHVDNERVDALARAGLEQSRRAGKPVTRPVATPVSAPV
                     KAAPVTVTSRPIVDIRHATVYRGETRVFSDFSFALREGEHAAIVGP"
BASE COUNT         2402 a         2958 c         3346 g         2262 t
ORIGIN      
        1 tgattacctg ggatgccgtg ccggacgcca tgtattacaa cttgtatttc atgacgacga
       61 aaggcgtgca gatcaagttt tcagaactca cccgccctat cgccagcgca gaggatttta
      121 aaaccgtcat cggcgtcacg aaggaaaaag ggacctgtat cgaaggggct cagtcgccct
      181 tcatgcatga cgatctggcg aacggaacct gttatcacta tgtcgtgacg gtggtgacgc
      241 agaaagggga aagccccgag tctcaagaag tcatggcgat tccagctccg tacctgattg
      301 cgaaagtgat cggccaggaa ggtgtggacg aaggagagtt cagctctccg acgggcatcg
      361 cagtggacaa ggacggcaat ctctacgtgg ccgatacgga caatcattcc attcagaaat
      421 tcgataaaga ggggaagttt ctcggccgtt ggggcggaga ggcgggtagc gcggaaggca
      481 attattatta tccccgcggc ctcacgacca ataacgacgg ccaggtctac gtagccgata
      541 ccggtaacaa tcgtgtgcag cggctcgata ccgacggcaa tcccatgaag tcctggggca
      601 agttcggatt tgcctggcgc ggggccgata tgaataagtt cgacgcgccc tggggtgtga
      661 ctaccgacca ggaaggcaat atttacgtca ccgataccaa caatgcccgc attcaaaaat
      721 tcaaaggcga cggcacgccg ttgctcaagt ggggacggga tggcagtttc gacggcgcgt
      781 tcttctttcc ccgtggagtc gcggtggact tcgttggaaa tacgtatgtg gccgatgaag
      841 gcaacaaccg tatccaaaag tttgatacgc ggggcagctt tttgaccaag tggggacgcg
      901 agggtagcgg tcccggtcag ttcaaggcgc cctggggcgt gacctgcgat gccttgggca
      961 acgtgtatgt agtggatcaa ggcaatcacc gcattcagaa gtttgacgga aacggcacct
     1021 tcctctgtgc ctggggcaat cgcggaaaaa cggaaggcca gctgaacttt ccgtcaggtg
     1081 ttgcggtcga taaggaaggc gcagtctatg tcgtggatag cggcaatcac cgcgtcatca
     1141 agtatgtgcc gaccgatgaa gaattgaatc gcgggaaaga agagcgtgaa cgggctcagg
     1201 tcgtcagcga ataccctgct cccggcaatc tggcgatcaa gcctggtgat acggaaacgt
     1261 tcctgagttg gatggaggtg ccgaaagcca cttcctacaa tctctatttc catacctccc
     1321 ccaacctgac cgtagaaggt ggtacgaaga tcgagggcgt gacgagtccc tacaatcata
     1381 ccgggctgac caacgaccaa gcctactttt atgcgttgac ggcagtctat gaggacggca
     1441 cagagagtac gttgtcggag gaagtttcgg cgacaccggt gctgatcgac atcacggccc
     1501 cgcaaacgcc gtatgccgtg atcaaccacg gcgccttcat gacgaactcg ccggaaattg
     1561 ttgtcacaat ttccgcaacc gacctcgata cgggcgttgc cgcgtactat atctccgaaa
     1621 atccgatgac cccgatggcg ggaacgcccg ggtgggtgga cgttccgccg gccatcaagt
     1681 tcggcgcgac gattcccttc attctttccc cgggcgatgg gcagaagacc gtcatcgtct
     1741 ggtttaagga tctcgggaac aacatttcga ccccggcaag cgccaccatt ctcgtcaata
     1801 catccggcta tctctgcgtc tccaagtggg gcaagccggg ccgcggcgca tcactattgc
     1861 acggcggcga gttcatggcg ccgatgtacg gtctggccat cgatcagcag ggatccattt
     1921 ttgtcgtcga caacggaaac aaccgtattc agaagtttga ccgtaacggc aatttcatca
     1981 ttctctgggg aaacttcggt gcggccaacg ccaactttca caatccgacc ggtattgcct
     2041 gcgatgccaa gggcgatgtc tacgtggtcg atacgaacaa tcaccgcgtc cagaaattcg
     2101 acggcaaatt gggcggctac atgatgaagt tcgggtcgcg tggaaacggc gaaggccagt
     2161 tcaatgcgcc gtggggcatc gcgatcgatc gggtgcgcgg ctatatctac gtcgtcgata
     2221 gtgcgaactt cagagtccag aaattcgaca tgaccggcga attcattatg tcgtggggaa
     2281 gtttcggcaa cggcgacgga cagttctatt tcccgcgcgg tatcgccgtc gatcaggcag
     2341 acggcaccgt ctatgttgtc gatatgggca accatcgcgt acagaagttc gacaccagca
     2401 cgaacgtcct gcctcagttg ttgacgaaat ggggaggaag ttctgaggcc ggccatgcca
     2461 gcagtccgtt ggcgcaagag gcggggcagc ttcgttcccc ctggggtatc gccgtggatg
     2521 gacaaggtga cgtgtatgtc acggataccg ggaaccatcg tcttgaaaag ttcgatcgtg
     2581 aggggaactt tatttctcag tggggtggct tcggcggcgg cgacggacaa ttcaacttcc
     2641 cctacggaat tgtggtggat tcacggggaa gcgtattcgc cgtcgatagc ggcaacacca
     2701 gagtgcagca atttatgccg gcagaagaag gcagcgagcg gttgcaggaa gaagcggatg
     2761 ccgcagccga actggggcag atcgataaaa ccacgagagt ctaagggcag cgggcaggaa
     2821 gcccgggaga gggtggcagc ggatgttaga gaaggagatt cggctcgggt tgacctatga
     2881 cgacgtcgtc ctggttccgg ctaaatcgca agttcttccg accgaagtcg atacccgcac
     2941 gcgcctctct cggaatatcc atctgaatat tcccatcgtg agtgctgcca tggacaccgt
     3001 gacggaagcg cggttagcca tcgcaatggc ccaagagggc ggcatcggca ttgtgcaccg
     3061 tgtgctctcg cctgccgacc aagcggctga aattgaccgg gtcaaaaagt ctgaaagcgg
     3121 gatgattctt gatcccatca ccatctcgcc tgatcaaacc atccgtgatg ctcacgacct
     3181 gatggcccga taccggattt ccggcatccc tgtgacgaag ggcggtaaat tggtcggcat
     3241 cctgacgaat cgcgatctgc gatttgagac gagaatggat ctgaaggtct cgcaagtcat
     3301 gaagcgtgac aagctcgtca ctgcgccgga aggcaccagc ctggagaaag cccgcgagat
     3361 cctgcacgag caccggattg aaaagttgcc ggtggtgaac aagcagttcg aactcaaagg
     3421 cttgatcacc attaaggaca ttgaaaagcg gatcaagtat cccaacgcct gcaaggatgc
     3481 ccacggccgc ttgcgtgttg gtgcggcgct tggcgtgggg cctgatacga aggatcgagt
     3541 ggacctgctg gtaaaggccg gcgtagacgt cgtcgtcgtg gataccgccc atgggcattc
     3601 ccaggcggtg cttgatacgg tcaaaatggt cagaaaagcc catccgaaac tggatatcat
     3661 tgccggcaac attgcaaccg cacaggcagc gaaggacttg gtgaaggcag gtgtggatgc
     3721 agttaaagtc ggcgtcgggc ccggttcgat ttgcaccaca cgaatggtgt cgggggccgg
     3781 catgccccaa ttgacggcca tcgccgattg cgccagggct ttagccggat cgggaattcc
     3841 ggtgatcgcg gacggcggga ttaagtattc cggcgatatc acgaaagcct tggcagcggg
     3901 cgcctcggtc gttatgctcg gaagtttgtt ggctggtacg gaagaagctc caggcgaaac
     3961 ggtcttgttc caagcgcgca cctacaaggt ttaccgtgga atggggtcca tcggggccat
     4021 ggagcgtggc ggtggggatc gttacgggca gggtggtcgt ccgacaccaa aactggttcc
     4081 ggaaggtatc gaaggccgcg taccgtacaa aggtcttctc gcgccgcaca tctatcagat
     4141 ggttggtggt gtgaaatccg gtatgggata ttgcggatgt aagaccattc ccgatttaca
     4201 acagaaggcg accttcattc gccagacatt ggccgggctc cgcgaaggcc atgtacatga
     4261 cgtgattatt accaaagaag cgcccaacta tcgcactgat tgggaataac acgcgtagaa
     4321 cgggcgagag gcgttgcctt ccccgttttc gtcagccccc gcactgaacc tctaatctcg
     4381 tacaccgcgc acactcatgg aactttggca cgatagaatt cttgttctcg atttcggctc
     4441 ccagtacacg caactcattg cacgacgtat ccgtgaagcg caggtctatt cgcagatcct
     4501 gccctgcacg gtttcgctcg cgacagtgtt ggcctatcgg cccaaaggta tcgtcctgtc
     4561 aggcggtccc tcgagtgtct acgacaagaa ggccccgaaa atccccaaac aactctttga
     4621 tcaaggcatc ccgatacttg gaatctgcta tgggatgcag ttggtgacgc atctccaggg
     4681 cggtgaagtg gagaaagcgc cgcaccgcga attcggccgg gccgagctgc agcttgacga
     4741 tcgctcggat ctcttcaagg gtattggacg tggtggatcc actgtggtgt ggatgtcgca
     4801 cggggatcgc atcgagcgga tgccgccagg tttccgctcc attgcccata cggacaattc
     4861 tcccattgcg gcgatgaagc ggcatgatgc caaacagcgt atttattgtt tgcagtttca
     4921 ccccgaggta gcccataccg ccgaaggtgc cacgattctc aagaattttg tctacgacat
     4981 ctgcggctgc aaaccgacct ggacgatgcg ctcttatgtc gacactgctg tggagcagat
     5041 tcgtcagcag gtcgggacgg agcgggtgat ttgtgccttg agcggtgggg tggattcgtc
     5101 ggtggcagcg gctctgacgc atcgtgcggt cggcgatcaa ttgacctgta ttttcgtcga
     5161 taacggggtg ttgcgtgccg gcgagcgtga gcaggtcgag aagacctttg cgtcccaact
     5221 acacctcaac atgcgcatcc tcgatcgtac gacccagttc atgacggcgt tgaaaaatgt
     5281 gactgatccg gagcgcaagc ggaagatcat cggccgcctc ttcatcaaga atttcgaagc
     5341 cgaatccaag aagctcaagg gcatcaaata tcttgtgcag ggcacgctgt atcccgatgt
     5401 catcgaaagc gtcagcttca aagggccttc cgcgaccatc aagacccacc ataacgtcgg
     5461 cgggttgccg accaaaatga aactgaagct ggttgagccg ctgcgcgaat tgttcaagga
     5521 cgaagtgcgg gtgttggggc aggagcttgg cttgccggat gagatcattt ggcggcagcc
     5581 gtttcccggt ccggggttgg cgatccgggt gctcggcgcg gtatccaaag aacgattggc
     5641 catcttgcgc ggggccgaga cgatcgtcga tcaggaaatt cgcgccgccg gactctatcg
     5701 ggaaatctgg caggcctttg ccgtgttatt gccgatccga actgtcggcg tgatgggcga
     5761 tcagcgaacc tacgagtatg tgattgcgat tcgggccgtc acaagcctcg atggcatgac
     5821 cgctgactgg gccaagattc cgaacgacgt actgggtagg atgtcgagtc ggatcatcaa
     5881 tgaagtgaag ggcgtcaacc gcgtcgtcta tgacatcagc tctaagcctc cgagcacgat
     5941 cgagtgggaa taacagtgct agatatgcca cctaaacggt gagtcacggc gatcccttta
     6001 cctgatacgt tgtccctttc acagcatatg accgttcgtc tgcaaaaact cattgccggt
     6061 accggcatcg cttcccggcg aaaagctgag gaattgattg tgtccggacg cgtgaccatc
     6121 aacggccacg tggtgaaaga gcttggcact aaagtcgatc ccgagcgcga tcatgtcaaa
     6181 gtggatggca aacatctgcg agccgcgcag ccctatgtct atttaatcct caataagccc
     6241 aagaacgtca tgtccaccct cgatgatccg gaaggtcgtc cgaccgtgaa ggattttttg
     6301 cgtggcgtga cggtgcgcgt gttcccggtc ggccgattgg atttcgatag cgaaggactc
     6361 atgttgctca ccaatcatgg tgatttagcc caggcgctgc tccatccccg ctaccatgtg
     6421 ccgaagacct atctcatcaa ggtcaagggt gtcctcgacg atacgaaaat cgagacgctt
     6481 gaacggggag tcaaactcga agatggtttc accgccccgg cgaagatcaa taaagtcagc
     6541 agggccgaaa gcaattcgtg gctggaagtc actatccatg agggccgcaa acaccaggtc
     6601 aagcggatga tcgaggcggt cggccattcg gtcatcaaac tgacacgtgt tcggatgggg
     6661 cctctcttgc tgggagactt ggcatcgggg gaatatcggt ttttaaccga ccgtgaggcc
     6721 aatcggttac gggaactggt ggaagatcgt gtggcaaggg ccgaacatcc ggagttggac
     6781 gcgtcgggca aaccggtgcg ctggatgccc cctacagagc ccgcccctcc acgcccacct
     6841 aagccggaac gttccgggcg tggccccaaa tctcaacggc cggacttcgg acctcgatca
     6901 gttcgaggtt cgggtggcgc gaactccggg cgtcctggtc gtggtgcgct gaaacgcccg
     6961 ctcagtcctg gggcgaagtt ccgccgtcct gcaaagacaa caggctttca acggcctcgg
     7021 ccgggtgcgg tccctcaagg cgacgatcgt gtcccaatgg cgaatcctcc cgattcggtg
     7081 aggaagcctc ggccttcggg agcaggtttt cagcggggct cattcgacag aaaatctcaa
     7141 ggcgctgggc gaaagacgcc agggaatcat tctggctcgg accagaaatt tcaacagaat
     7201 cggcgaagcg ggccttctgg ctcgcgccca ggccagcggg gccctgcaag gcagtcgcgg
     7261 aattcaacgc ggagaagagc atgaagatca ggacccatcg gtgcggggag ttgaccaagg
     7321 cacacgtcgg tcaacaggtc gtattgaacg gctgggtgca acggcgtcgt gaccacggca
     7381 tggtgctgtt catcgatttg cgtgatcgga caggattgac gcaggtcgtg ttcaacgctg
     7441 agcggaacgc cgctgttcat caggcggcac atacactgcg gagtgaatgc gttgtgtcgg
     7501 tcaccggtca ggtcatggct cgccccgagg agtctcgcaa cccgcatttg ccgacgggcg
     7561 agattgagat cttcgtcgat gccgtggaga ttctgaacga agcgaagacg ccgcctttca
     7621 tgattgaaga cgacatcgaa atcaccgagt ccctccggtt gaaatatcgc tatctcgacc
     7681 tgaggcgccc gcgaatgcag cggttgctgg gattgcgtca cgggatcatg caggccgtgc
     7741 gtgcattttt aaatgccgag cattttctcg aagtggaaac accggtcctg acgaagagca
     7801 ctccggaagg cgcgcgggat tatctggtgc cgagtcgcgt caatccgggc atgttctatg
     7861 cgctgccgca gtcgccgcag ttgtttaagc aagtcttaat ggtcagtggt gtcgaccggt
     7921 attaccagat tgctcgttgc ttccgtgacg aagatctgcg caacgatcgc cagccggaat
     7981 ttacccagat cgatcttgaa atgtcgttcg tagatcgcga gcaggtcatg tccctcatgg
     8041 agcggatgat cgtgtctgtg ttcaaggatg ccggtggcgt gcagttgccc gtaccgtttc
     8101 cacgaatgac ctatgccgaa gccatggggc gttatggctc cgacaaaccg gatctgcgct
     8161 tcgatatgcc gttgtacgat gtcacggcct ttgcggcgaa gagtgacttt aaagtgttta
     8221 aagacgcggc gaccaaaggc gggctcgtca aggctttgat tgtgtcgggc ggggcgacca
     8281 tcgcgcggag ccgtatcgat gcgcttggtg agacggccaa aagtttcggc gccaaaggct
     8341 tagcttggct gaaggtcacc gcggaagggc aattggaatc cgtgattgcc aaatttctcg
     8401 atgcccaggc ctttgccgcg gccttgccgg aggcaaagcc cggggacctg gttcttttcg
     8461 gagcggataa gccggccatc gtgcatgacg tgctggggcg gatccgcttg ttgctgggtg
     8521 aagaactgaa tttgatcgat aaaactgcct ggaagcccct ctgggtgacc gagttcccat
     8581 tgctggacta ttcgccggaa gagaagcgct atatcttcat gcacaatcct tttgcggcgc
     8641 cgatggacga agatatcccg ttcctcgaca cggagccatt gaaggccaga gcgaaggcgt
     8701 acgatatggt gctcaacggg agtgaaatcg gcggcggtag catacgtaat catcgcagtg
     8761 atattcagtt tcggattctc gacctgttgg gtatcgggaa ggatcaggcg caggccaagt
     8821 tcggcttctt gctggaagcc ctggagtacg gcgcgccgcc gcacggcggg atcgccttcg
     8881 gcttggatcg attgatcatg ctcctgggcg gggccgattc tattcgtgat gtcattgcct
     8941 tcccgaagac ccaacgcgcg cagtgcccgc tcaccgaggc tccatctgct gtcagtaccg
     9001 atcagttgct ggaacttcgc attaagctgg atcttgtcga atagcaggag ccatcccatt
     9061 catggccggc aacacgttgg gccgagtctt caccgtcacc tcattcggcg aaagccatgg
     9121 accggccatc ggctgcgtcg tcgatggctg tccgccgggg ctggcactct ccgccgaaga
     9181 tattcagcag gatctggatc gccgcaagcc cggcacgtcc cgccatgtca cgcagcggca
     9241 ggaatccgac cacgttgaaa tcctatccgg ggtgttcgaa ggacggacga cgggcacgcc
     9301 gatcgccctc ctcatccgaa acgaagatca gcgcagccgg gattatggga acctcatcga
     9361 caccttccgt cccggccatg cggactacac ctattggcag aaatatggca tccgcgatca
     9421 tcgggggggc gggcggtcct cagcccgtga aaccgctgtg cgggtggcgg ccgctgcgat
     9481 cgcgagaaag tggctatacg aaacgcatgg cgtcatgatc cgtggctatc tcagtcaact
     9541 gggcccgatc gaggctccgt ttcaaagctg ggacacggtg agcaggaatc cgttcttctc
     9601 tgccaacgcc gaggtggtcg aacggctcga ctcctttatg gacgaattgc ggaaagcggg
     9661 tgactctgtc ggtgcgaaaa tcacgacggt cgcggagcat gtgccggtag ggtggggtgc
     9721 tccggtgtat gccaaactgg atgcggacct ggccggcgcc atgatgagca tcaatgccgt
     9781 gaaggcagtg gaaatcgggg ccggttttgc ttcagtcgcc cagcgaggtt ccgagcatgg
     9841 ggacgaactc acgcctgagg gatttgtcac caatcatgca ggcgggattc tcggtggaat
     9901 ttcaaccggg caggatgtgg tggtgaccat cgccatcaag ccgacctcga gcattcgcgt
     9961 gccgcgtcgt tcgatcgata aaaccggcaa gccacagtcc gtcgaaacca atggccgtca
    10021 cgatccctgt gtcggtattc gtgccactcc tattgccgaa gccatgatgg ccctcgtgct
    10081 catggaccat gccctcctgc atcgcgccca aaacgccgat gtgaaaactg cgactccgaa
    10141 gatcgccggc tccatcaaca ggcttggttc ttcagccagc tcaaaagcat cctcgaagcc
    10201 caacgctggc cctaccgaag tatagcggtc tgtcatgcct gcgtcgttta ctcgcttctg
    10261 ctcgtgatga cacgggggag atatccgtgg atgcgcactg aggagagtga ttggtgaagt
    10321 ggttggtgag cgcttagagg aacgtcctcg catgatcgag atctacacag acggcgcctg
    10381 cagcggaaac cccggccctg gtggctgggg agttttgttg cgcctggacg gggaagaaac
    10441 ggaactctgt ggaggcgagc cggccacgac caacaaccgg atggagttgc tcgcggtcat
    10501 agaggcgctg cagtggttta cgcacccggt gaaggctcga gtctacacgg attctcagta
    10561 tgtgcaaaag ggcatcagcg aatggatcca tagctggaag cgccggggct ggaaaactgc
    10621 cagcaaagag ccggtcaaaa atgaagatct ctggcgtcgt ctggatgcat tggcttccgg
    10681 ccacaccatc gaatggcatt gggtcagggg acacaatggc catgtggaca acgaacgagt
    10741 ggacgcgctg gctcgcgccg ggctcgaaca gtcgcgtcgt gccggaaagc cggttacgcg
    10801 tccggttgcg acaccggttt ccgctcccgt caaggcggca ccagtcacag taacttcccg
    10861 ccccattgtg gatattcggc acgcgaccgt ttatcgagga gagacccgag tcttctcgga
    10921 tttttccttc gcgctgcgtg aaggagaaca tgcggcgatc gtcggtcc
//
LOCUS       DAOQEW010000129        10770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1884_length_10770_cov_7.924312, whole genome shotgun sequence.
ACCESSION   DAOQEW010000129 DAOQEW010000000
VERSION     DAOQEW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_10770_cov_7.924312"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLS35_13415"
     CDS             <1..458
                     /locus_tag="PLS35_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HNI19716.1"
                     /translation="LEKWAVQLGGGKNHRFSLGDGVLIKDNHLAVLRSTGIDVAGACR
                     LARAGAPHGLRIEVEAKTMQEVKEALAGKADIILLDNMSPAQVRQAVALIKQRALVEV
                     SGGMTLQTVAAMAQAGADFISVGALTHSAPAANLSMDLSAQRGRRGRSH"
     gene            442..1314
                     /locus_tag="PLS35_13420"
     CDS             442..1314
                     /locus_tag="PLS35_13420"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNI19717.1"
                     /translation="MGGPISDSPISPPFDIDRLQASLRTHAFGRPLRYSASTASTNAD
                     ALASLQQSATKPAPHGLVILADCQTAGRGRRGRTWHSPPQGNLYLSVIVVPRPVATGT
                     TPWLTWIPLLSALAGADCLSQQTGLPVSVKWPNDLLIHDKKVGGILCEQTTTPDRTMA
                     IIIGIGLNINAPLNTFPEDLQQGATTLAAEAGHEFDRVAILADLLFRLEQRLNRLFLE
                     GPAGMIDEFTQRCSTVGQTVRVTLEEKGMVQGIAESIGPDGCLRLRVTSDESPSLPST
                     LLEVRSAEVVHLRG"
     gene            1350..2120
                     /locus_tag="PLS35_13425"
     CDS             1350..2120
                     /locus_tag="PLS35_13425"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNI19718.1"
                     /translation="MLLAIDIGNTNVVCGLFEGSTLRGHWRMATESRRTDNEYGILLL
                     NLLQNAGFTPDQITGCVLSSVVPALTATFDSVVQEYFHRTAVIVGPETDSGLTLRYAN
                     PKEIGSDRIVNAAAAYARYHSDVIIVDFGTATTFCAVTKSAEYLGGVIAPGLGISADA
                     LFARTAKLPKVEIIRPKTVIGKDTIGGIQSGLLFGYVGLVDGIVRRMERELGRTSRVV
                     ATGGLATVIAQETETIQEVLPFLTLEGLELLYRRNRLA"
     gene            2249..2812
                     /gene="grpE"
                     /locus_tag="PLS35_13430"
     CDS             2249..2812
                     /gene="grpE"
                     /locus_tag="PLS35_13430"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNI19719.1"
                     /translation="MQSMSEDEKNVHSIDNLDSSDEPSSATIDGASELQQVLDAKAEE
                     CKAAHEKYLRLAAEFDNYKRLAQRDQREQIKFGNEQILKELLPVVDNLERAIKSAKGA
                     GSVDALTEGVELTLKQLVGALSKFGVKAVESVGQVFDPATQQAVAQVPSDTIPENHVV
                     EEYQKGYLLQDRILRAAMVTVSTGAAN"
     gene            2895..4817
                     /gene="dnaK"
                     /locus_tag="PLS35_13435"
     CDS             2895..4817
                     /gene="dnaK"
                     /locus_tag="PLS35_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNI19720.1"
                     /translation="MGKVIGIDLGTTNSCVAIMSGGDPVVIANAEGSRTTPSVVGITD
                     KNERLVGQIAKRQAITNPENTIFSVKRLMGRKFKSKEVQEAMKRLPYKVVEADNGDAH
                     VELRGKRYSPPEVSAMILQKMRQTAEDYLGEKVTEAVVTVPAYFDDSQRQATKDAGQI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSVLEIGDGVFEVKSTNG
                     DTYLGGDDFDERVMDWLVEEFKKDQGIDLRKDRMALQRLKEAAERAKIELSSSQESEI
                     NLPFITADASGPKHMVTKLTRAKLEQLVDDLIQKTIEPCRKALADAGVSAKDIQEVVL
                     VGGMTRMPKVIQVVKDFFGKEPHRGVNPDEVVAIGAGVQGGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIERNTTIPTKKSQVFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGIPPAPRGMPQVEVSFDIDANGIVHVAAKDLATQKEQSIKITASSGLSKEEVD
                     KLVKDAQSHTEEDKKRRRVAEARNQADSLLYSTEKNLTEHGDKIGEDDKTKITEAIAS
                     LRKAMEGDDPAAIESATQALTTASHKLAEEMYKKASASAGPTDAGASAGDGGAQAKTD
                     EKVVDAEFEEVDKDKK"
     gene            4898..6010
                     /gene="dnaJ"
                     /locus_tag="PLS35_13440"
     CDS             4898..6010
                     /gene="dnaJ"
                     /locus_tag="PLS35_13440"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNI19721.1"
                     /translation="MAKRDYYETLGIERNATDDEVKKAFRKLARQHHPDLHTSPEQKK
                     SAEEKFKELNEAYEVISDPDKRRRYDTFGHAGGPQGAEGFDFGGQGGFGDIFNDIFED
                     FFGQPRGRARAERGNDLQYNLEITFEESVFGKEAKLKIPRWETCADCKGTGAKSATAI
                     KMCPACKGQGQLRFQQGFFSVSRPCGQCEGVGQIITEPCQVCGGRQRIRKERMLSVNI
                     PGGIETGMRLRLANEGEHGVQGGPQGDLYVAVTVKPHPHFRREGQDIACDLPINLVTA
                     ILGGRVEVPTLKGNTFMKIPAGTQPDKILRLKGLGFPNLKGGHTGDQLFNVKVEIPTK
                     LTSRQKELLEEFAKESGYTKDSEGEGFLDKMKTFFE"
     gene            6047..6784
                     /locus_tag="PLS35_13445"
     CDS             6047..6784
                     /locus_tag="PLS35_13445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNI19722.1"
                     /translation="MPAFFISSAEIHGTALTLTGELCHHLRASLRVKPGEVLRLTDEH
                     RQRYEVRVSAVAPQALTAEILESRPGPIDTSPRVILAQVILKGDHMDWVVQKASELGV
                     KAVLPLISRHGVVRPQPERVAAQVARWQRIATEAAQQSEQWQPPQVLEPLEVRRFFSG
                     HQATTALLLAERQNAVGLANVPLPALPTDTITVIVGPEGGWAEEELSQAMDGQCLAVS
                     LGEHILRADTAAVTALSIVQSRLGRLG"
     gene            complement(6807..7778)
                     /locus_tag="PLS35_13450"
     CDS             complement(6807..7778)
                     /locus_tag="PLS35_13450"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNI19723.1"
                     /translation="MRTLAAVVCSLLVVLCVGTVEAGSPTVPVTVAMQKSGSEATLLG
                     MFFHDHQLGWAVGSGGTILKTTDGGHKWKKVSSGTSSLLTAVYFRDAKRGWVVGANGT
                     VRTSKDGGETWSVVVVSTQAPLYGIAFASPTKGWMVGGNGTILQTTDGGENWIDQASG
                     TSAALYAIALTNELQGTAVGALGTILTTADGGKNWTTQVSQSSATFFDVAFADDTNGW
                     AVGNAGALFQTIDGGTHWVDRTLPCGRTCNKLTDLIRVRFTDPQQGWIVGERGQILRT
                     SDAGFNWVEETSPVKRSLMALSFPRATAGWAAGEGGTIISISAAGNK"
     gene            complement(7790..9463)
                     /locus_tag="PLS35_13455"
     CDS             complement(7790..9463)
                     /locus_tag="PLS35_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNI19724.1"
                     /translation="MKYITCAILGVALVANSVDSKAAPAEPPALHPNAAVPGATLSIT
                     GKGFGPFKSTRFNQVTFQGVPALIQRWDSDLIEVRVPSQAANGPVDIIIGKKHVKAGS
                     FTRLQPVIHSVSPAEAEPGSMLEIAGEHFGNTAGPRDPNTIFGVNSVAVGDVSVRVRK
                     WRDDKIEVELPGNVQSGDVVIRMSSSDPLPDGSCCAPVKQVVSNAMPMKVLASVRVDP
                     SSGPVGTKVVLFGKGFGTTRNPEDGVLFGGHLATVSQWTDTTVVVHVPLDAQTGPVVM
                     KRNGQERAIGTYTVQTPKATALTPTEAPIGTLLKITGENFGFYSEAGSTPFNYIDFSL
                     SENTVEIGGVQAIVYRWGHDRIDVWVPFSAKSGPVVVKRAANTPKPDGTCCADKQVLE
                     TQVGNFTLVTPKIDSYSPNTGGLDEVVTIKGSGFGKFLKTAEPSKVITDSVYARVAPE
                     LGENVSRTEVLFNGVGAIVQSWTDTEIKVKIPHRHSYGVGKLGEFNPDLTSGPLVVRR
                     GSWDLLPDGSCCTPKKWVTLEAGTFTIQPSGLPDASYWRNPNPENSHFH"
     gene            complement(9706..10167)
                     /locus_tag="PLS35_13460"
     CDS             complement(9706..10167)
                     /locus_tag="PLS35_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNI19725.1"
                     /translation="MSIDEKLSSLGLTLPVAPKPVASYVPAVLAGDLLYLSGILPFRD
                     GQVAITGKLGQDVTVERGAEAARLALLNALAVVKQELGSLDRVQRIVRVVGHVASATG
                     FVQQPAVINGASDLLVQIFGEAGRHARVALGAAELPLHAAIELELLVQVKA"
     gene            10392..10673
                     /locus_tag="PLS35_13465"
     CDS             10392..10673
                     /locus_tag="PLS35_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19726.1"
                     /translation="MPSTMVQVPILMSPGQKRRLAQKAKAANLTMAELLREGGERYVP
                     ADNPTLLDHMAKQVIRETKKTIRAIDKTLALVAESEARMLALSKTRKKG"
BASE COUNT         2475 a         3267 c         2998 g         2030 t
ORIGIN      
        1 ccctcgaaaa gtgggcggtg cagctgggag gcggcaagaa tcatcgattt tctctgggcg
       61 acggtgtgct catcaaggac aaccacctcg ccgttttgcg ctcgaccggc attgatgtgg
      121 caggagcctg tcgcctcgcg cgagccggtg cacctcatgg ccttcgtatc gaggtggaag
      181 ccaaaaccat gcaggaagtg aaagaagcct tagcgggcaa agccgatatc attctgctcg
      241 acaatatgtc gcctgcccag gtgcgacagg ccgtggcact catcaaacaa cgcgccttgg
      301 tcgaagtgtc tggtggaatg accctgcaga cggtcgccgc catggcccag gccggtgcag
      361 atttcatttc ggtcggcgcc ttgacccatt ccgcgcccgc agccaacctc agcatggatc
      421 tctcagcaca gcgaggacgc cgtgggcggt cccattagcg attcgccgat ttcccctcct
      481 ttcgacattg atcgcctcca agcctccctg cgcacccacg ccttcggcag accgttgcgc
      541 tattcagcct cgaccgcgtc caccaatgcc gatgctctcg catccctgca acaatcggcg
      601 acaaagcccg cccctcatgg attggtcatt cttgctgatt gccaaacggc gggacgagga
      661 cgtcggggac gaacctggca ttcaccgccg cagggcaatc tgtatctctc ggtcatcgtg
      721 gtacctcgac ctgtcgccac aggcacaaca ccctggctga cctggattcc gctgttatcc
      781 gccctcgcgg gagccgactg cctctctcaa cagacagggc ttccggtttc cgtgaaatgg
      841 ccgaatgatc tgctcatcca cgacaagaag gtcggcggca tcctgtgcga gcaaaccact
      901 acgccagatc gaacgatggc catcattatc ggcatcggtc tgaacattaa tgcgccgctc
      961 aacacctttc ctgaagatct tcaacaaggt gccaccaccc tggccgcaga agccggccat
     1021 gaatttgatc gcgtggccat tctggctgac ctgctgtttc gactggaaca acgactgaat
     1081 cggctgtttc tcgagggtcc cgcggggatg atcgatgaat ttacccaacg ctgctcgacc
     1141 gtgggccaaa ccgtccgggt caccctcgaa gaaaagggga tggttcaagg aatcgccgag
     1201 tccatcggcc ctgatggctg cctccgcctg cgggtgacat cggacgagtc cccgtccctt
     1261 ccctcgacgt tgttggaagt ccggagcgca gaggtcgtcc atctgcgggg gtgaaatcct
     1321 gctacggctt ggggtagagt gaaggcacga tgctgttggc aatcgacatc ggcaacacaa
     1381 atgtagtctg cggcctgttc gaaggctcga ccctgcgcgg gcattggcgc atggccaccg
     1441 aatccagacg taccgacaat gagtatggca tcctgctgct gaatctgctc caaaacgcgg
     1501 gctttacccc ggaccagatc accggctgtg tcctctccag tgtcgttcct gcgctcaccg
     1561 ccaccttcga ttccgtggtg caggagtatt ttcatcgcac cgccgtcatc gtagggcctg
     1621 agactgactc cggcttgacg ctgcgctacg cgaaccccaa agaaatcggc agcgaccgta
     1681 tcgtcaacgc cgccgcggct tatgcgcgct atcactcgga tgtcattatc gtcgacttcg
     1741 gtaccgctac gacgttctgc gccgtaacca aatcggcgga gtacctcggc ggcgttatcg
     1801 caccgggtct cggcatttca gccgacgccc tgtttgcccg caccgcgaaa ctgccgaaag
     1861 tcgaaatcat caggcccaaa accgtcatcg gcaaggatac gatcgggggc attcaaagcg
     1921 gcctcctgtt cggctatgtg ggactggtcg acggcattgt ccgacgcatg gaacgggaac
     1981 ttggccgcac ctctcgagtc gtcgccaccg gcggtctggc gaccgtcatt gcacaggaga
     2041 ctgaaacgat ccaggaagtc cttcccttcc tcaccctcga aggcttggaa ctgctgtacc
     2101 gccggaatcg cctcgcttag gcgcctttcc gacgggtgct gtcacaacat tccatcccgc
     2161 gaacgctgag agactcgaaa aaacgactat ttcttttcat tccccgttga cttccatcct
     2221 ccggtggcta tctcactcac cagaaaaggt gcaatcaatg tctgaagacg agaagaacgt
     2281 tcacagtatc gacaacttag atagttcaga cgaaccctcc tctgcgacga ttgacggtgc
     2341 aagcgaattg cagcaggtgc tggatgcgaa agccgaggag tgcaaagccg ctcacgaaaa
     2401 gtatctgcga ctggcagccg aattcgacaa ttacaagcgg ctggcccaac gcgaccaacg
     2461 cgagcaaatc aaattcggca acgaacaaat tctgaaagag cttctccccg tcgtcgataa
     2521 cttagaacga gccatcaaat ccgccaaagg ggcgggctct gtggatgccc tcacggaagg
     2581 cgtcgagttg acactcaagc aactcgtggg tgccctatca aagttcggcg tcaaagcggt
     2641 ggagagtgtg ggccaggtgt tcgatccggc tacccaacag gcagtcgcgc aagtgccgtc
     2701 ggacacgatt cctgaaaatc acgttgtgga agagtatcaa aaaggatatc tcctccaaga
     2761 tcgcatcctc cgggcagcca tggtcaccgt atcgaccggg gcggcgaatt aagacggtac
     2821 gtacgcaact tgtttcgcga gcacagctga cacgatcgtt cacgtattga tattttttgc
     2881 gaaggagcca acccatgggc aaagttattg gaatcgacct cggcacgacg aactcctgcg
     2941 tcgccatcat gagcggtgga gacccggttg tcatcgcgaa tgcggaagga agtcggacca
     3001 ctccgtcagt ggtgggcatc acggataaga atgagcgctt ggtgggacaa atcgccaaac
     3061 ggcaagccat tacgaatcct gagaacacca tcttctccgt gaagcgcctg atgggacgca
     3121 aattcaagag caaggaagtc caggaagcca tgaagcgcct gccctacaag gtcgtggagg
     3181 cagacaatgg cgacgcccac gtcgagttgc ggggcaaacg gtacagcccg ccggaagtct
     3241 cggccatgat cctgcagaag atgcggcaga cggctgaaga ctatctgggc gaaaaggtca
     3301 cggaagccgt ggtgacggtt cccgcctatt ttgatgacag ccaacgccag gcgaccaagg
     3361 acgccgggca aatcgccgga ttgaacgtcc tccgcatcat caacgaaccg acggcggcct
     3421 cgctcgccta cggtctcgat aagaaaaaag atgaacgcat cgccgtgtac gacttgggtg
     3481 gcggcacctt cgacgtgtcc gtgctcgaga tcggcgatgg cgtgtttgaa gtgaagtcca
     3541 cgaacggcga cacctacctc ggtggcgacg atttcgatga acgcgtgatg gactggctcg
     3601 tcgaagaatt taagaaggat cagggcatcg acctccgcaa ggaccgcatg gcgctccaac
     3661 gcttgaagga agcggccgag cgggcgaaga tcgaactgtc ctcctctcag gaaagcgaaa
     3721 tcaatctgcc gttcatcacg gcagacgcca gcggcccgaa gcacatggtg accaagctga
     3781 cccgcgcgaa attggagcag ctggttgacg atctcattca gaagaccatc gaaccctgcc
     3841 gcaaggcgtt ggctgatgcg ggagtgagcg cgaaggatat ccaggaagtc gtcctggtgg
     3901 gcggcatgac ccgtatgccg aaagtcattc aggtcgtgaa ggactttttc gggaaagaac
     3961 cccaccgcgg cgtgaacccg gatgaagtcg tggccatcgg cgccggtgtt caaggcggcg
     4021 tactgaaggg cgaagtcaag gatgtgctgc tgctcgacgt gactccgttg tcccttggca
     4081 tcgaaaccct cggcggcgtc tttaccaaac tgatcgagcg gaatacgacg attccgacaa
     4141 agaagagcca ggtgttctca acggcggccg acaatcaaac cgccgtaacg atccgggtct
     4201 tccagggcga acgtgagatg gccaatgaca acaagctgct ggggcagttt gacctcgtcg
     4261 gcattccccc ggccccacgc ggcatgccgc aggtggaagt ctccttcgat atcgatgcca
     4321 atggcatcgt ccacgtcgcc gccaaagatc tggcgacgca gaaagagcag tccatcaaga
     4381 tcacggcgtc cagcggactc agcaaagagg aagtggacaa gctggtcaag gacgcgcagt
     4441 cccatactga agaagacaag aaacgccgtc gggtcgctga agcccgcaat caggcagatt
     4501 ctctgctcta cagcaccgag aagaacctga ccgaacatgg cgataagatt ggcgaagacg
     4561 acaagaccaa gatcaccgaa gccattgcca gcctccgtaa ggcgatggaa ggagatgatc
     4621 ccgccgccat cgaatcggcc acgcaagcat tgaccacggc ctcccataaa ctggccgaag
     4681 aaatgtacaa aaaggcttca gcttccgctg gtcctaccga tgccggcgct tcagccggag
     4741 atggtggggc gcaggccaag accgacgaaa aggtcgtgga cgcggaattc gaagaagtcg
     4801 ataaggataa gaagtaagct cctcactgca ggcaggacca ggcttcacgg acggtgaggc
     4861 ttggtcctgt tgcacttaga acgggtaacc ctcttccgtg gcaaaacgcg attattacga
     4921 aaccctcggt atcgaacgga acgcgaccga cgacgaagtc aaaaaggcct tcagaaaact
     4981 cgctcgccaa catcacccgg acctgcatac ctcgcccgag cagaagaaat cggccgaaga
     5041 gaaattcaaa gagctgaacg aagcctacga ggtcatcagc gatccggaca aacgtcgccg
     5101 gtacgataca ttcggccacg ccggcggacc gcagggcgcc gagggattcg atttcggcgg
     5161 ccagggtgga ttcggcgata tcttcaacga cattttcgaa gacttctttg gtcagcctcg
     5221 tggccgtgcc cgggccgagc gcgggaatga ccttcaatac aatctcgaga tcaccttcga
     5281 agagtcggtc ttcggcaagg aagccaagct caaaattcct cgctgggaaa cctgtgcgga
     5341 ctgtaaaggc accggcgcaa aatccgcgac cgccatcaag atgtgtcctg cctgtaaagg
     5401 acagggacag ttgcgtttcc aacaaggatt cttcagcgtc agccgcccgt gcggtcagtg
     5461 cgaaggcgtc ggacaaatca tcaccgaacc gtgccaggtc tgcggtggac gtcagcgcat
     5521 ccgcaaggaa cgcatgctgt cggtcaatat tccaggcggt atcgaaaccg gcatgcgcct
     5581 gcggctcgcc aatgaagggg agcatggtgt gcaaggcggc ccgcagggcg acctctatgt
     5641 cgccgtgacg gtcaaacccc atccccactt ccgccgagaa gggcaggata ttgcctgtga
     5701 ccttcccatc aatctcgtga cggccatcct cggcggccgt gttgaagtgc ccacgctcaa
     5761 gggcaacacg ttcatgaaga ttcctgcagg cacgcagcct gataaaatac tgcgactcaa
     5821 ggggctcgga ttcccgaacc tcaagggtgg ccataccggg gatcaactgt tcaatgtgaa
     5881 agtggagatt cccacaaagc tcacctcgcg gcagaaggaa ctgctggaag aattcgccaa
     5941 ggaaagcggc tacacgaagg atagcgaggg cgaaggcttt ttagacaaaa tgaagacgtt
     6001 tttcgaatag accgggcttc tgcaccttcc agatccgccg accgtcatgc cggcattttt
     6061 tatctcctca gccgagatcc acggaaccgc actcaccctt accggcgaac tctgccacca
     6121 tctgcgtgcc agcttgcgcg ttaagcctgg cgaggtcctg cgactcaccg atgaacaccg
     6181 tcagcgatat gaggtgcgcg tgagcgcggt ggctccgcag gcgttgaccg ccgagattct
     6241 cgaatctcgt cccgggccga tcgatacgtc gccacgagtc atcctcgcgc aagtcatact
     6301 caagggcgat catatggatt gggtcgtgca gaaggcgagc gagttgggcg tgaaggctgt
     6361 tctcccgctg atctcacggc atggcgtcgt acgaccgcaa ccagagagag tggcggccca
     6421 ggtggctcgc tggcagcgga ttgccaccga agccgcacag cagtcggaac aatggcagcc
     6481 gccacaagta ttggaaccgt tagaagttcg ccgctttttc tccggccatc aagccaccac
     6541 agcacttctg ttagcggagc ggcagaacgc cgtggggttg gccaacgttc ccctaccggc
     6601 tcttccaacc gataccatca ctgtgattgt tggccctgaa ggcggatggg cggaggagga
     6661 actgtctcag gctatggacg gacagtgcct ggccgtaagt ctgggggagc acatccttcg
     6721 agcggatacg gcggcggtca cagccttaag tatcgtgcag agtcgattgg gacgattagg
     6781 ttagacggta gacaggcggc gagtggttac ttattgcctg ctgcgctgat ggaaatgatg
     6841 gtccctccct cgccggcggc ccagccggcc gtggcacgag gaaaactgag cgccatcaga
     6901 gaacgtttca ccggactggt ctcttccacc cagttgaacc cggcatcgct ggttcgcagg
     6961 atctggccgc gttccccaac gatccacccc tgctgcggat ccgtgaaccg tacacgaatg
     7021 agatccgtga gtttgttgca ggtccggcca cagggcaagg tgcgatcgac ccaatgggtt
     7081 cccccatcga tcgtctggaa taacgccccg gcattgccga ccgcccatcc gttggtatca
     7141 tccgcaaagg ctacatcaaa aaaggtcgcc gagctttggc tgacctgtgt ggtccagttc
     7201 tttccgccat ccgccgttgt gagaatcgtg cccaacgcgc cgacggctgt gccttgcaat
     7261 tcgttggtca gcgcaatggc atacaacgcc gcgctcgtgc cgctggcctg gtcgatccag
     7321 ttctctccgc cgtccgtggt ctgcaaaata gtgccgttcc cacccaccat ccagcccttc
     7381 gtcggagatg caaaggcaat cccgtacaaa ggcgcctgag tggacacaac gacgacactc
     7441 cacgtctcgc caccatcctt gctggttctg accgtgccat tcgcccccac gacccatccg
     7501 cgcttcgcat ctcgaaagta aacggcggtc aggagtgacg acgtgccgct ggagaccttc
     7561 ttccacttgt ggccaccgtc ggtagtcttg agaatcgtcc cacctgatcc aacggcccac
     7621 ccgagctggt ggtcgtgaaa aaacatgcct aatagcgtcg cttcggagcc gcttttctgc
     7681 atcgcgacgg tcaccggaac ggtaggagaa ccagcctcga ccgtgccgac gcagagcacg
     7741 accaagagcg aacacaccac tgcagccaaa gtacgcaacg taaagtgcct caatgaaagt
     7801 gactgttttc aggattcgga tttcgccaat aactggcatc cggcagaccg gacggttgaa
     7861 ttgtaaaggt gccggcctcc aacgtgaccc attttttggg cgtgcagcaa gaaccatccg
     7921 gtagcaaatc ccacgaccct ctccgcacga ccagcggccc ggatgtcagg tcaggattga
     7981 attccccaag tttccctact ccgtaggagt gacgatgagg gattttgacc ttgatctccg
     8041 tatccgtcca ggactggaca atcgctccga ccccattgaa caagacctcg gtacgcgaga
     8101 cattttcacc caactccggg gccacgcggg catacacact gtccgtgatg accttgctcg
     8161 gctcggctgt cttcaaaaac ttaccgaacc cgctcccctt gatggtcacg acttcatcga
     8221 gtcccccggt gttcggactg tacgaatcga ttttcggcgt cacaagggta aagttaccga
     8281 cctgcgtctc cagcacctgt ttatcagcac agcaggtgcc atccggcttc ggcgtgttgg
     8341 cggcgcgctt caccaccacc ggcccgctct tcgcgctgaa gggcacccag acatcgatgc
     8401 ggtcatgtcc ccatcgatac acaatcgcct gcacaccgcc gatctcgacg gtgttttcgc
     8461 tcaatgaaaa atcgatatag ttaaacggtg tcgacccggc ctccgaataa aatccgaaat
     8521 tctctcctgt aattttcaac aacgtaccga taggcgcctc cgtcggcgtc agcgccgtcg
     8581 ctttgggtgt ctgcacggta tatgttccga tggcgcgctc ctgcccgtta cgcttcatca
     8641 caacaggacc ggtttgagcg tcgagcggca catggaccac gacggtcgta tccgtccact
     8701 gcgacaccgt ggccaaatgc ccgccaaaga gtacgccatc ctcaggattt ctggtggttc
     8761 cgaagccctt gccaaacagc accactttgg tgccgaccgg gccgctcgaa ggatcgaccc
     8821 gcacagacgc caggaccttc atcggcatcg cattgctcac cacttgcttg accggagcac
     8881 aacacgatcc gtccggcaag ggatcggatg aggacatgcg aatcaccaca tcgcccgact
     8941 gcacattccc cggcaactcg acttcaattt tgtcgtcacg ccacttgcgc acgcggacgc
     9001 tcacatcgcc gacggccacg ctattgacgc cgaagatcgt attcggatcg cgcgggcccg
     9061 cagtgttgcc gaaatgctct ccggcaattt ctaacatcga gcccggttct gcttcagccg
     9121 gtgatacgga gtgaatcaca ggctgcaacc tggtgaacga accggctttc acatgcttct
     9181 taccgatgat gatatcgacc gggccattgg cggcctgcga cggcacccgc acctcgatga
     9241 gatccgaatc ccaccgctga atcagggcag gcacgccttg aaacgtcacc tgattgaacc
     9301 gggtcgattt gaagggaccg aaccctttgc ccgtgatcga cagtgtggca cccggcacgg
     9361 ccgcattggg atgcagagcc ggaggttcag ccggggctgc cttggagtca acggaattcg
     9421 ccactaaggc tacgccgagg atggcacaag ttatatattt catgggatcc actccctacc
     9481 gatacccttt gaatgagcct gacgagcagg ccgggtgagg atgaaatgaa tgatggtacg
     9541 ccagatgaac gccccgcacg tggcacaaaa ccccacgcca ctgaggcaac gaagccggat
     9601 ggggggtgtg gtatcaaacc agaagacttc cggactataa caagcggttt tttttgatgt
     9661 caaggcaagc aacgacgagg gaaaggacag cgcagaacgg gtacgtcagg cttttacttg
     9721 aaccagtagt tcgagttcga tggcggcatg caatggcaac tccgccgcgc caagcgccac
     9781 acgtgcatgg cgtcccgcct caccgaaaat ctgcaccaat aaatccgatg ccccattgat
     9841 cacggcaggc tgttgcacaa agccggtggc cgaggcgaca tggcccacga ctcgcacgat
     9901 acgctgcact cggtccaacg accccagttc ctgtttcacg accgccaagg cgttcaggag
     9961 cgccaatcga gccgcctcgg ctccccgctc aaccgttacg tcctgtccca gcttgcctgt
    10021 aatggctacc tgcccgtccc gaaacggcaa gataccgctc agatacagca aatcaccggc
    10081 aagcaccgcc ggcacataac tggcaaccgg cttcggcgca accggaagtg tgaggccaag
    10141 gctgctgagt ttctcgtcaa tcgacacggt atgatccttt cctgttcagc accccaccga
    10201 aggtcaggta tgcaagagcc cacgtagtgc aactcgaaca ttgagcccta cgtatacgtc
    10261 atccgagggc gattgagcaa gatgaccccc tccagcatgc atcataaacc gctgaggaca
    10321 tggggcgcat cgacagcata tccgatccta tgatatatat gggctatata tccacgccga
    10381 gggaggcctc catgccatcc acgatggtcc aagttcctat cctcatgagc cccggccaaa
    10441 aacgccggct ggcacaaaag gcgaaagccg ccaacctcac catggcagag ctacttcgag
    10501 aagggggcga gcggtacgtt ccagccgaca atccgacact cttggatcac atggccaagc
    10561 aagtcatccg ggagacaaag aaaaccattc gcgccatcga caaaactctc gccctcgtgg
    10621 cagagtccga agcgcgcatg ctggctctct ccaagacccg taagaaaggg tgacgatggg
    10681 actcaccgaa cgcgtgtggg atgccttcgc caacaccatc aagctgaacg ataaagtgga
    10741 gcaactcaac accaccgtcg ccaagcagca
//
LOCUS       DAOQEW010000130        10768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1885_length_10768_cov_8.942406, whole genome shotgun sequence.
ACCESSION   DAOQEW010000130 DAOQEW010000000
VERSION     DAOQEW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_10768_cov_8.942406"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="secD"
                     /locus_tag="PLS35_13470"
     CDS             <1..755
                     /gene="secD"
                     /locus_tag="PLS35_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNI19727.1"
                     /translation="AKGAREFDRITGENVKKRMAIVLDNTIYSAPVIQERISGGRAQI
                     TGTFSMEEANNLSIVLRAGALPAPLKIIQDLTVGPSLGQDSIEKGIKATLFAGLLVIV
                     FMAIYYRLSGVIANFALMLNLICLIGSLAALNATLTLPGIAGIILTIGMGVDSNVLIF
                     ERIREELRQGKAVRVAIDAGYDKALLTIVDSHVTTLITGFALFLFGTGPIKGFAVTLC
                     LGIAINLFTALVGTKVIFDLFNQRKKIEQLSI"
     gene            768..1685
                     /gene="secF"
                     /locus_tag="PLS35_13475"
     CDS             768..1685
                     /gene="secF"
                     /locus_tag="PLS35_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNI19728.1"
                     /translation="MFEILGKTNIDFMGKRTISFALSGILAFLGIIAVLQIARGAANL
                     GIDFAGGTAVQLKFDQSIRIDEARRALESNGLGSAELQEFTQDNKLLIRVKASTTIEE
                     KVAERVMAVFSKEFPGNKFVVDSSMEIGPTIGKKLQEDAMIAVLISFVGIVLYIAVRF
                     ELRFGVAAALATFHDVLAVLGVFYILDKEITLLVVTALLTLAGYSLTDTVVVFDRIRE
                     NLRTRRREDEETIINQAINQVLSRTIVTSLTVVIVLIPLVLAGAEVLHDFSLALLGGV
                     MFGTYSSIFVASPLLLLWPGSAGSLLKRR"
     gene            1761..4937
                     /locus_tag="PLS35_13480"
     CDS             1761..4937
                     /locus_tag="PLS35_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI19729.1"
                     /translation="MLFWSRSHSLQRKIITAIVMVGLLPLGLSLVLTYLEERRALRET
                     IGANFKEVAVEAARRIEMQVTRGINEAQQLAATPFLRTSVTESNRTYLDKDEKTVQSM
                     IKAWQQRWRQRDKQSEFPLFINRIVTNYLIRWHDIRKTDYVGIFVTDNRGALVVSSIP
                     QVEYYYGKTAWWQAFMKRGTGKVFVSDIFFDPAFGTHVVNVSAPIIDDEQHTTIGAVT
                     VLLRRDTLFHSVSEATIGQTGHAMLFSSDGAPMICPILSPEEHVVKPELVAAINSSAA
                     GWTTAENDSHGGKNSIVGFAPVRLGADLTPESLGGKRWVTFVRQDPAESYAPLSRLVV
                     QVTVYGLGVFAVLVLTGLTVARRIARPIGLLHEGVQKIGSGRLDQQLDLKTGDEIEQL
                     ADAFNKMAANLRVSFEQIEHRMEDVRRLEARYRDLIEHSPEMIYQLNKAGQFVHVNKT
                     GLDKLGYTLEEMLQMRLWDLVSKGREAEVLAYLERLVAQGRSTIETVFVAADGRPIDV
                     EIHSTALFEIGGGLVHSRAFVRDVTERRLLEEQIHRYTTRLEQAVNERTQQLVASQER
                     YKALFDLVADSVFMVGEDGVIVAVNKREEQALGYSEQQVVGHSILDVILPAHHEDMRS
                     LLAKIHSGERQVPTQEITVRDAGGKETPVEMDVILVRGSDHTWVMVQLRDITDRKRLE
                     RQMHTYQQELEAKVSERTREIEETKQYLENLLENANDVIYTLDSEQCFTYVNSKVLAW
                     GYAKEDLLGRPYLGLLSKRHRGRRLKSTLDIGVKQVYEVEVLTKSGEPRSVMVSVSPL
                     HGVDGEILGVLGIARDMTDTKKLEQQIRNSEKLASVGKLAAGVAHEINNPLGGILNCL
                     YNMRKGTLSPSRQEEYLASMEDGLRRVQRIVRQLLDFSQQHNPELALADINQVVNRVL
                     LLTDHLFVPHRVQLETDLAVDMPEIMIDRHMMEQVLMNLVLNAIQAMRNGGVLTIRTT
                     VKEAHCLVRVQDSGCGISSSVLPRIFDPFFTTKNEGEGTGLGLSVSLGIVERHGGRIV
                     VESEVGKGTTFTVSIPLSTERYAIGRFS"
     gene            4934..6385
                     /locus_tag="PLS35_13485"
     CDS             4934..6385
                     /locus_tag="PLS35_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI19730.1"
                     /translation="MKGLKIFIVDDEPLMRLSMQDALEGVGCEVMAAATGTEGVAVLG
                     TRQFDIVITDLRLPGADGLTILKTCKERSPTTEVILITAHGSVDTAVGAIKLGAYDYI
                     TKPFQMDELLLIVERVGKIIGLRRENLELKEVLEDRFSFGGIHGRNHQMRALLDKIKL
                     IAAADSPVSLVGERGTGKELVAHTIHLNSPRRDQPLIKMCCADLPDHLLEAELFGHEK
                     GAVPGASRQRRGRFELAHKGTLLLDDVDSLSPLLQSKLWRVLQERKFERVGGRESIEL
                     DVRILCASRQDLKEAVAQGRFRQDLCDHLAAVQVVVPPLRERREDVLVIAEHVLELRS
                     KSLNKSPKGFSQPSCDLLLHYSFPGNVGELEQMVERAVTLGRNGEPLQPWDLCGFQSC
                     PYLGGAPQATCGFCDEGLSAKADEPAAQAATTLATAREAFEKDYIETVLKQVDGSRTS
                     AAAVLGLSRKALWDKCKRYGISSAKGEAEERDE"
     gene            complement(6387..7493)
                     /locus_tag="PLS35_13490"
     CDS             complement(6387..7493)
                     /locus_tag="PLS35_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNI19731.1"
                     /translation="MSNSTTTPPTIPSAAPAPTQGASAQAPAIDHLEYWKTGFRELKT
                     FRGHSHGVWTVAYAPDGQTIVSGGVDRYVRIWDIETGRLLRSLRGHTADIRALVFTPD
                     GRTLASGSEDRTIRLWNPKTGEPTKLLFTRYDHNVCSLSLSPDGLMLARGSHNKDIKI
                     WEVTTGTDLMTLLGKDQYDHHWSVCVAFSPDGVHLASGSDIGKIRIWEVLPSGEEKIL
                     HNGHWEETAEDSTETRGFFIEDDGGFQKPMEFWIGAMTFTPDAKILITGSRDNTIRFF
                     EMPTMNELRVLRGHNGWVRSLTVSPDGKVLISSGDDNTIRFWDIATGRNFRTDKTHTG
                     PVRGVALSPDGLRLASASWDRTVKLWEGGQEPAE"
     gene            7690..8721
                     /locus_tag="PLS35_13495"
     CDS             7690..8721
                     /locus_tag="PLS35_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19732.1"
                     /translation="MTTGVEVSKPSMEIRFQPALLQEVIDSFIEKTEREGDPTYYKEF
                     HELADPIYEKFTLDDREAEFKKLYQYLFGIWGFSDIIRDAFNEFPDLKARVGVVLVKG
                     VLKEDQEGVDVLRKWGSVEHDLAKQFEEKGLKGVGIKLIPRRFYDPALTRYCRHELMH
                     INDMLDPAFGYDPDTRVGQNPGEETLILHRYRILWNISVDSRLIAAGKEPMLQKEDRF
                     KEFRSWYRKIPPAQLKSVFEGLWQTSYFTHSELVEMATDTLRILDRAVDVEGGEIPET
                     QNKVMLMPGFPCPLCRFPTYTWVEDMETKLEPYILDFIRENHPGWDVEYGACDRCVEV
                     YKLRADGVM"
     gene            8734..9339
                     /locus_tag="PLS35_13500"
     CDS             8734..9339
                     /locus_tag="PLS35_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19733.1"
                     /translation="MAGDPKYGRRDFLKDSVVSVAKAAREFAIHKDAPREQPAAPVRT
                     DWLRPPGAVDESTFLERCTRCSDCIKACPPGAIVSDLSNGTPVIFANQVACELCEDFP
                     CIAACATDALLPVADVVEVRMGIATVSHRVCTAGQGCHACVSKCPVDALSMDFHALRL
                     DVEQARCVGCGMCEQICKTVNDRIAVKVVPARNLSAGSLNY"
     gene            9549..10526
                     /locus_tag="PLS35_13505"
     CDS             9549..10526
                     /locus_tag="PLS35_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HNI19734.1"
                     /translation="MTSKQVVQPTSPSSTAIAPPKAVPIKDSPAVERALNRSKIYLLF
                     SWSLLYPEDEEFLDYLQCGEFVEDGRAALDGLRLALDGIGGDRAIQKIALMKKQFDQI
                     EKLVSAECVNWQIGDLQTEHRRVFTNVITLDCPPYETLFGNDHVFAQSHVMGDIAGFY
                     KAFGVELSKDVHERLDHLSVELEFMHFLTYKESYSRCHDGIDKTEIVVDAQKKFVKNH
                     IGRWVPLFCRMLAKKSDTGLFKLIADCMSEWMDFEVAFLGVTVQPYSEADYRPATFNA
                     PEGQTYECGAQDKGNELSMLLSEVGAESFMDQKTKEKDGEKSEGPVGTA"
BASE COUNT         2285 a         2764 c         3274 g         2445 t
ORIGIN      
        1 atgcaaaggg ggcacgggag ttcgatcgga tcacgggcga aaacgtcaag aagcgtatgg
       61 cgatcgtgct cgataacacg atctattcgg cgccggtcat tcaggagcgt atcagcggag
      121 gaagagccca gatcacggga acgttctcga tggaagaggc caacaacctg tcgatcgtac
      181 ttcgggcggg cgcgctgcct gcgccgctca agatcattca ggatctgacc gtcggtcctt
      241 cgctcggaca ggactcgatc gagaaaggca tcaaggcgac tctgttcgcg gggcttctcg
      301 tcatcgtatt catggccatc tactaccggc tgtccggggt catcgcgaat ttcgcgttga
      361 tgctgaatct catctgtttg atcggatctc tggctgcgtt gaatgcgacg ctgaccttgc
      421 ccggcatcgc cggtattatt ttgacgatcg gaatgggtgt cgattccaac gtcttgatct
      481 tcgaacgcat ccgtgaggag ttgcggcaag gcaaagcggt ccgggtggcc atcgatgccg
      541 gatacgataa agccctctta accattgtcg actcgcacgt cacgacattg atcaccggtt
      601 ttgcgctgtt tttgttcggc accgggccga tcaaagggtt tgcagttacg ttatgccttg
      661 gtatcgcgat caatctgttt accgcgttgg tcggcaccaa ggtcattttc gacctcttta
      721 atcagcgcaa gaaaattgag cagctcagca tttaggaagg agccggtatg ttcgagattt
      781 tgggaaagac gaatatcgat tttatgggga agcgcacgat ctcgtttgcg ctatccggca
      841 tcctggcctt cctgggtatt attgccgtgc ttcaaatcgc gcgcggggca gccaatctgg
      901 gaatcgattt tgcgggcggg acggcagtcc aactcaaatt cgatcaatcg atcagaatcg
      961 acgaagcccg gcgagcattg gaatcaaatg gcctgggatc ggccgaattg caggaattca
     1021 cgcaggacaa taagttgctc attcgtgtga aagcgtcgac gaccattgag gagaaggttg
     1081 cggaacgggt gatggccgta ttcagcaagg agttccccgg caacaagttt gtggtggatt
     1141 ccagtatgga gattggtccg acgattggaa agaagctcca agaagatgcc atgattgccg
     1201 tgttgatttc attcgtgggg atcgtgctct acatcgcggt ccgatttgag ctgcgattcg
     1261 gcgtcgcggc ggcgctggcg acgttccacg atgtcctcgc ggtattgggc gtgttttaca
     1321 ttttggataa agaaatcacc ttgctggtgg tcacagcgct ccttacgctg gccgggtact
     1381 cgttgacgga cacggtggtc gtgttcgatc ggatccgaga gaatcttcgc acgcgccggc
     1441 gggaagacga ggaaacgatc atcaatcagg ccatcaacca ggttttgagc cgcacgattg
     1501 ttaccagttt gaccgtggtg atcgtgctga ttccgctggt gttggccggc gcggaggtct
     1561 tgcacgattt ttcgctggcc ttgctcgggg gagtcatgtt cggtacctat tcgtccatct
     1621 tcgtggcaag cccattgctg ctgctgtggc ccggcagcgc ggggagcctc ctgaagcgtc
     1681 gctaggcctc ggctcgtcag agccgggcct agggccccac ctcctgtgcg acagggccat
     1741 tagtcggggt atccgcacgc atgctgttct ggagccggtc acacagcctc cagcggaaaa
     1801 tcatcacagc cattgtgatg gtcggtctcc tgccgttggg tctttctctt gttttgacgt
     1861 acctcgaaga acgccgggcc ttgagggaaa ccatcggggc caacttcaaa gaagtcgccg
     1921 tagaggcggc ccggcggatc gagatgcagg tcacgcgcgg aatcaacgaa gcgcagcagc
     1981 tcgccgccac accgtttctt cgcacctctg tcaccgaatc caatcgcact tacctggaca
     2041 aggacgagaa gaccgtccag tcgatgatca aagcctggca gcagcgctgg cggcaacggg
     2101 acaaacaaag cgagtttccg ctgttcatca accgcatcgt gaccaattat ttgattcgct
     2161 ggcacgatat ccggaaaacg gactacgtgg gtattttcgt gaccgacaac cgcggcgcgc
     2221 tcgtggtcag ttccattccg caggtcgagt actactacgg caagaccgcc tggtggcagg
     2281 cgttcatgaa gcggggaacg gggaaggtgt tcgtcagtga tatcttcttc gatcctgcct
     2341 tcggcacgca tgtggtcaat gtgtcggcgc caatcattga cgatgaacaa catacgacca
     2401 tcggcgcggt gacggtgctc ttgcgtcgag ataccttgtt tcactccgtc tctgaggcga
     2461 cgatcggcca gacaggacat gccatgctgt tcagttctga cggcgcgccg atgatctgcc
     2521 cgatcctgtc tcccgaagag catgtggtca aacccgagtt agttgcagcc atcaactcga
     2581 gtgcggccgg ctggacgact gcggagaatg attcgcatgg cggaaaaaat tcgatcgtcg
     2641 gttttgcccc ggttcgcctg ggggctgatc tcacacccga gagtctgggc ggtaaacgct
     2701 gggtgacgtt tgtgcggcag gaccctgctg aatcctacgc gcctctctct cggctcgtcg
     2761 tacaggtgac cgtctacggg ctcggagtct ttgccgtatt ggtcctcact ggcctgacgg
     2821 tcgctcggcg catcgcgcgt cccatcggcc tgctgcatga gggcgtgcag aaaatcggga
     2881 gtggccgctt ggatcagcag ttggacctta agaccggtga tgaaatcgaa cagcttgccg
     2941 atgccttcaa taaaatggcg gcgaacctgc gcgtgtcatt cgaacagatc gaacatcgta
     3001 tggaggatgt tcgacgtttg gaggcgcggt atcgcgatct catcgagcat tcgccggaga
     3061 tgatctatca gctgaataaa gccgggcaat tcgtacatgt gaataaaacc ggtttggata
     3121 agctggggta cacgctggag gaaatgcttc agatgcgtct ctgggatctg gtttccaagg
     3181 ggcgtgaggc tgaggtattg gcgtatctgg agcgactcgt cgcgcagggg cgtagtacga
     3241 ttgagacggt gtttgtggca gcagatggac gtccgatcga cgtggaaatt cattcgacgg
     3301 cgttattcga gatcggcgga gggctcgttc actcccgcgc ctttgtgcgc gatgtgacgg
     3361 aacggcgtct actggaagaa caaattcacc gctacaccac gcgactcgag caggcagtca
     3421 atgagcggac ccaacagctg gtggcctcgc aggagcggta taaagcgctc tttgatctcg
     3481 tggccgattc tgtgttcatg gtgggcgaag acggcgtgat tgtcgcagtc aacaagcgtg
     3541 aagagcaggc gctcgggtat tccgaacaac aggtcgtagg gcacagcatt ctcgacgtca
     3601 tcctgccggc tcatcacgag gacatgcgtt cgctgttggc gaagatccac tccggcgaac
     3661 ggcaagtgcc cacgcaggaa atcacggtcc gtgatgccgg cggcaaggaa acgccggtgg
     3721 agatggatgt gatcctggta cgcggcagcg atcacacgtg ggtgatggtg cagctgcgtg
     3781 acattacgga tcgaaagcgc cttgaacgcc agatgcacac ctaccagcag gagctggaag
     3841 ccaaggtcag cgaacgcacc cgtgagatcg aagagaccaa gcagtatctg gagaacctgc
     3901 tggaaaatgc caatgacgtc atttataccc tcgatagtga gcagtgcttt acctatgtca
     3961 acagcaaggt attggcctgg gggtacgcga aggaagattt gctgggacgg ccatatttgg
     4021 ggctgttgtc gaaacgtcat cgtgggcgcc ggttaaaatc gacgttggat atcggcgtga
     4081 agcaggtcta tgaggtcgaa gtcctcacga aatccggcga gccccgttct gtcatggtca
     4141 gcgtatcgcc gctgcatgga gtcgatggcg agattttggg ggtcctgggc attgctcgcg
     4201 acatgaccga caccaagaaa ctcgagcaac aaatcagaaa ctctgaaaag ctggcatccg
     4261 tgggcaagtt ggcggcaggg gtggcccacg agatcaacaa cccgttaggt ggtatcctga
     4321 actgtctcta caatatgcgt aagggcacgc tgtctccgtc acgccaggaa gaatatctcg
     4381 cgtcgatgga agatggattg cgtcgcgtac agcggatcgt ccggcaactc ctcgactttt
     4441 cccaacagca caatccggag ctggcactgg cggatatcaa tcaggtcgtc aaccgcgtgt
     4501 tgttgttaac cgaccacctg ttcgtgcctc atcgggtgca gctggagacg gacctagccg
     4561 tcgacatgcc ggaaatcatg atcgatcggc atatgatgga acaggtgtta atgaatctcg
     4621 ttttgaacgc cattcaggcc atgcgaaacg gcggggtact gaccattcgc acgacggtca
     4681 aagaggctca ttgtctcgtg cgcgtccagg attccgggtg cggcatttct tcgtccgttc
     4741 tgccgcgcat tttcgacccg tttttcacca cgaaaaacga aggtgaaggg accgggcttg
     4801 gtttgtcggt gagtttaggc atcgtcgagc ggcacggtgg acggattgta gtagagagtg
     4861 aagtgggaaa gggcacgacc tttacggtgt cgattcctct ctcgaccgaa cgctacgcca
     4921 ttgggaggtt ttcgtgaagg ggctcaagat ttttattgtc gatgatgaac cgctgatgcg
     4981 gctctctatg caggatgcgc tggaaggggt cgggtgcgag gtcatggccg ccgcgactgg
     5041 cacggaaggg gtggctgtcc tgggcacgcg ccagtttgat atcgtgatta ccgacttgcg
     5101 tctcccgggc gctgacggct tgacgatttt gaagacctgt aaggaacgaa gtcccacgac
     5161 cgaagtcatt ttgattacgg ctcacggatc agtggacacg gcggtggggg ccatcaagct
     5221 tggcgcgtac gattacatta ccaagccgtt tcagatggac gaattgctgc tgatcgtcga
     5281 gcgcgtcggc aagattatcg ggttgcggcg ggaaaatctc gaactgaaag aagtgttgga
     5341 ggatcgtttc agtttcggtg gcatccacgg gcgcaatcat cagatgcggg cgctgctgga
     5401 caagatcaag ctcattgcgg ccgctgattc gccggtttct cttgtcggtg aacgtggaac
     5461 ggggaaggag ttggtggcgc ataccatcca tttaaacagc cccaggcgcg atcagccctt
     5521 gatcaaaatg tgttgtgccg accttccgga tcatctgctg gaggcggagc tcttcggcca
     5581 tgaaaagggg gcggtacccg gtgcctcgcg gcagcgacgg ggacggttcg aactcgcaca
     5641 taaggggacc ctgctgctcg acgacgtaga ttcgctgtct cctctcctgc agagcaagct
     5701 atggcgggtc ctgcaggaac ggaagtttga gcgtgtcgga gggcgtgaat cgatcgagct
     5761 cgatgttcga atcctgtgcg cctcgcggca ggatttgaag gaggcggtag cgcagggacg
     5821 gttccgtcag gacctctgtg atcaccttgc tgccgttcag gtggttgtgc cgcctttgcg
     5881 cgagcgtcgg gaagatgtgc tggtcattgc cgagcacgtt cttgagctgc gatcaaaaag
     5941 cctcaataaa tcccccaagg ggttttctca gcccagttgc gacttgcttc tacactattc
     6001 tttcccgggc aatgtcggcg agttagagca gatggtggag cgggcggtga ccttggggcg
     6061 aaacggggag cctttgcagc cctgggacct ttgtggattc caaagctgcc cgtatctcgg
     6121 tggtgcgcct caggcgacat gcgggttttg cgacgagggc ctgtctgcaa aggctgacga
     6181 acctgcggca caggccgcta ccacattggc gacggcgcgg gaagcctttg aaaaggacta
     6241 cattgaaacg gtgttgaagc aggtagacgg cagtcgcaca agcgcagctg cggtattggg
     6301 attgtccagg aaggccctgt gggacaagtg caagcggtat ggcatctcct ccgccaaggg
     6361 tgaggcggag gagagagacg agtaaattac tctgccggct cttgaccgcc ttcccacagt
     6421 ttcacggtgc gatcccagga agcgctggcc agtcgcagtc catcaggcga aagagccact
     6481 ccgcgcacgg ggccggtgtg agttttatca gtccggaagt tccggcctgt ggcgatgtcc
     6541 cagaaccgga tcgtgttgtc gtcaccggag ctaatcagga ctttcccgtc cggtgacacg
     6601 gtcagggagc gcacccagcc attgtgtccg cgcaggacgc ggagttcgtt catggtcggc
     6661 atttcaaaaa accggatggt attgtcccgg ctgccggtaa tcaggatctt ggcatcgggc
     6721 gtaaaggtca tcgccccgat ccagaactcc atcggttttt gaaatccgcc gtcatcctcg
     6781 atgaagaagc cgcgcgtttc cgtcgaatct tcagccgttt cctcccaatg tccattatgg
     6841 aggatttttt cttcgccgct cggcaacact tcccagatgc gaattttgcc gatgtcggag
     6901 ccactcgcga ggtgcacgcc atccggtgag aaggccacgc agaccgacca atggtggtcg
     6961 tattgatcct tgcccagaag cgtcatgaga tcggttccgg tcgtgacttc ccagatcttg
     7021 atgtctttat tgtggctgcc gcgggccagc atgaggccat cgggggacaa tgacaaactg
     7081 cacacattgt gatcatagcg agtaaacaag agcttggtcg gctccccggt tttcgggttc
     7141 cacaggcgaa tcgtccggtc ttcgctccct gaggccagcg tacggccatc cggcgtgaag
     7201 accagggctc gaatatcggc cgtatggccg cgcaacgagc gcagaagacg tccggtttca
     7261 atgtcccaaa ttcgaacgta acgatccacg ccgccactga cgatcgtctg accgtccggt
     7321 gcataggcga cagtccacac gccatgcgaa tgtccgcgga aggttttgag ttcccggaag
     7381 cctgttttcc aatactctaa atggtcgata gccggtgctt gagcacttgc cccttgtgtg
     7441 ggcgccggtg cggccgaagg aattgtcggg ggcgtggtcg ttgaattgct cataaaagtc
     7501 tgtgcctcgt ccctggtgtg accgagggct atctagccct ggccagccct ccgcggtttg
     7561 caaaaaacgc cgaggggttt gtataatcgg cggtcgtttt gaggatcgta agagaaggac
     7621 cgtgagcaag tcaagcacag tgcttacgca tggtttctcc cttcactgta acggaaaatg
     7681 tgaggtctta tgactaccgg agtagaagta tccaagccgt ccatggaaat tcgatttcag
     7741 ccggccttac tgcaggaagt catcgactcc tttattgaaa aaaccgagcg ggaaggggat
     7801 ccgacctact acaaggaatt tcacgaactg gccgatccga tctatgagaa atttacgctg
     7861 gatgatcgtg aagcggagtt caagaaactg tatcaatact tattcgggat ctggggcttt
     7921 tccgacatca tccgtgacgc cttcaacgaa tttcccgacc tcaaggctcg agtgggagtc
     7981 gtcctggtca agggtgtgct gaaggaggat caggagggcg tcgacgtgct tcgcaaatgg
     8041 ggatccgtcg agcacgatct ggccaagcaa ttcgaggaaa aggggctgaa gggcgtcggg
     8101 attaagctga ttccacgccg cttctacgat cccgcgctga ctcgctattg ccgtcacgaa
     8161 ctgatgcata tcaacgatat gttggatccg gcgtttgggt acgatcctga caccagggtt
     8221 ggacagaacc ccggcgagga aacgctcatt ctgcaccgct accgcattct ttggaatatc
     8281 agtgtcgata gccgtctgat tgctgcgggc aaggagccga tgcttcaaaa ggaggatcgg
     8341 ttcaaggaat tccgctcctg gtatcgcaaa attcccccgg ctcaattgaa gtctgtgttc
     8401 gaagggttgt ggcagacgag ctacttcacc cattcggagc tggtagagat ggcgaccgat
     8461 accctccgca ttttggatcg cgccgtcgat gtcgaagggg gagaaatccc ggagacccag
     8521 aacaaggtca tgttaatgcc ggggtttccc tgtcccttgt gccgatttcc gacgtatacc
     8581 tgggtcgagg atatggagac gaagttggag ccatacattt tggacttcat tcgtgaaaac
     8641 catcctggct gggacgtcga gtacggagcc tgtgaccgtt gcgtggaagt ctataagttg
     8701 cgtgccgacg gtgtcatgta gtcggggtgc aacatggccg gtgatcccaa atacgggcgt
     8761 cgtgatttcc tgaaagattc tgtggtctcg gtggccaagg cggcgaggga atttgctatc
     8821 cataaggatg cgcctcgcga acagccggca gcccccgtgc ggacagactg gttgcgccct
     8881 cctggtgccg tcgacgaatc taccttcctc gaacgctgca cacgctgcag tgattgcatc
     8941 aaggcctgtc ctcctggggc gatcgtaagc gatctctcga acggaactcc ggtgattttc
     9001 gccaatcaag tcgcctgcga gctctgtgag gattttccct gtattgccgc atgtgcgacg
     9061 gatgcgctct tgccggtggc tgatgttgtc gaagtgcgga tgggcatcgc cacggtgtct
     9121 catcgtgttt gtacggcggg gcagggatgc cacgcctgcg tatcgaagtg ccctgttgat
     9181 gccctgtcta tggattttca tgcgctccgt cttgacgtgg agcaggcgcg ctgtgttgga
     9241 tgtggtatgt gcgagcagat ttgtaagact gtcaatgacc gcattgcggt caaagtcgtc
     9301 ccggccagaa atctttctgc gggcagtctt aactactgaa tcagtcgtgt gttaagaaat
     9361 gtcgaggcat tcatccatca tgttatttca tgaaacatcg gcacggaagc aatggcaaag
     9421 gagagagagt tcatgcttga catccctccc tcctttacct atcatacgcc tcctgtgccg
     9481 caggactcag atgttgcgta gtgtgcgcaa aaaatgcgca acatgttgag gtgaggagca
     9541 agatacgtat gacatcgaaa caagtcgtgc agcccacctc cccgtcctcc accgcaattg
     9601 ctccccccaa ggcagttccg atcaaggatt cgcctgctgt cgagcgcgcg ctcaatcgca
     9661 gcaagatata tttgttgttc tcctggagcc tgttgtatcc ggaggatgag gagtttttgg
     9721 attacctgca gtgtggtgag ttcgtcgaag acggacgcgc tgcgttagac gggttgcggc
     9781 tcgcgttgga cggcatcggt ggcgatcgtg ccattcagaa gattgccctg atgaagaagc
     9841 aattcgacca gatcgagaag ttggtgtcgg cggagtgcgt caattggcaa attggcgatc
     9901 tgcagaccga acaccgtcgg gtgttcacca atgtgatcac gctggattgc ccgccctacg
     9961 aaaccctctt tgggaacgac cacgtgttcg cgcaatccca cgtcatgggt gacattgcgg
    10021 gattctacaa agcgtttggg gtggagcttt ccaaggatgt gcatgaacgt ctggaccacc
    10081 tcagtgtcga actcgaattc atgcatttcc tgacgtataa ggaatcctat tcgcgttgcc
    10141 acgatggaat cgataaaacg gaaatcgtgg tcgatgcgca aaagaagttt gtgaaaaacc
    10201 acatcggccg ctgggtgccg ctgttctgcc ggatgttggc aaaaaaatca gacaccgggc
    10261 tgttcaagct tattgccgac tgtatgtcgg aatggatgga ttttgaggtc gccttccttg
    10321 gggtaaccgt gcagccatac tccgaggcgg actatagacc agctaccttc aatgctccgg
    10381 aaggtcaaac ctacgagtgc ggggcgcagg acaaggggaa tgagctcagt atgttgctga
    10441 gcgaagtcgg cgctgagtcc ttcatggacc agaagacaaa agagaaggac ggcgagaaga
    10501 gcgaaggccc agtcgggact gcgtaggagt tctcggtttt cgggcagtgc agtattcggt
    10561 tttcggtaga cgttatacgt tcccagttct ttttcttatt aatctttcag aggagggcat
    10621 acgcaatgag actggtgcag acacgcaaca agcgtttggt gtttggcatt ctgttctctg
    10681 cgctgatcgt cggcattatg ttgacgatcg ggcaggtgcc tctcgcggtc agccaaccgg
    10741 tgaccattcc cgtgaaggcg atcaaggg
//
LOCUS       DAOQEW010000131        10687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1897_length_10687_cov_8.564240, whole genome shotgun sequence.
ACCESSION   DAOQEW010000131 DAOQEW010000000
VERSION     DAOQEW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_10687_cov_8.564240"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLS35_13510"
     CDS             complement(<1..384)
                     /locus_tag="PLS35_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19735.1"
                     /translation="MIFPQPQLLLVDDSTLNLKILREFLPERQYRCTTAQDGRQAWDM
                     LEAEPDRFHAVLLDRIMPGMDGMEVLHKMKQHDALNQIPVIMQTAASTHQQTLEGLQA
                     GAYYYLATPFDQATLQAIVDAAVRDH"
     gene            complement(571..1248)
                     /locus_tag="PLS35_13515"
     CDS             complement(571..1248)
                     /locus_tag="PLS35_13515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI19736.1"
                     /translation="MRHVTGAVSAVVLLISSLSWAAASEPANDEQKTLYALGVAISQS
                     LTPFTLNEAELEFVKSGIADGVLKRSQKVDMNVYGPKIQQMQQVRSNALADVEKKAGA
                     AFLAKAAAEPGAKKTESGAIVTTIKEGKGATPKATDTVKVHYHGTLTDGTVFDSSVKR
                     GEPATFPLNQVIKCWTEAVQLIKVGGKSKLVCPSGIAYGDRGSPPVIKPGATLVFEVE
                     LLDIVKQ"
     gene            complement(1559..2503)
                     /locus_tag="PLS35_13520"
     CDS             complement(1559..2503)
                     /locus_tag="PLS35_13520"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNI19737.1"
                     /translation="MTPQPQFPNEHTESGEFQRQEDAFRQWVTDDGRSGLPAAAGRYH
                     LYVSLACPWAHRTIIVRALKQLESVVGMTVVDPIRDEQGWAFRTGQGHSIDPINGFHF
                     LSEAYHLTDPTYRSRVTVPVLWDTVTKRIVSNSDDDLMRMFNAEFNRFTISTLDLYPA
                     ALRADIDEMNTFLYEQVNNGVYKAGFATSQRGYEQAARSLFAALDQLDTRLSQRRYLF
                     GPQFLESDWRLFVTLIRFDAVYHGHFKCNVRRIIDYPHLSGYLKDLYQVPGIADTVNL
                     DHIKRHYYVTHDDINPTQIVPIGPQLDLESPHGRDKFS"
     gene            complement(2599..3108)
                     /locus_tag="PLS35_13525"
     CDS             complement(2599..3108)
                     /locus_tag="PLS35_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19738.1"
                     /translation="MAEDPPHPSLFQNVGFGLGLGWTHNLGARRVESVSVTNRIVRID
                     DERNDLVRAMPEVHLWIDRWDEQRWSWGPFLAVAPGSRIIDAVGGGLMVGYRPHRSDQ
                     YSFNFGIGGILDLDTQVLGDGLVANEPLPPRETSARTKHTTGAGLLVLFSVGWDLSAP
                     HQVSPPGRK"
     gene            complement(4790..5296)
                     /locus_tag="PLS35_13530"
     CDS             complement(4790..5296)
                     /locus_tag="PLS35_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19739.1"
                     /translation="MVRLTTHRLESPRQWWTSLAMGCSVLLFALPVFAAESSGPWECS
                     NYTGDAHTRCLQAFIEIQREKISKLEAEVQLQQSTVGQLKDQADRQNAMTAELQRQMS
                     QQSSAPSTYGYVSPGIAPGLLYGYPSVGFGLYLGRPWIYGSPFYARPYAWGPRYYRPY
                     YGRWHRRW"
     gene            5462..6412
                     /locus_tag="PLS35_13535"
     CDS             5462..6412
                     /locus_tag="PLS35_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNI19740.1"
                     /translation="MGEKQVIAVVGATGMQGGGLVRAILANPASGLAVRALTRDVNSD
                     KAKELARLGAEVVAADVHDVESLKRAFSGAAGAFCVTFFWAHLSPEKEYAEAEAMAKA
                     AKAAGVRHVIWSTLEDTRRWVPLSDNRMPTLMGKYKVPHFDAKGEADQLFKQLGLPTT
                     FLLTSFYWDNLIHFGMGPKPGPDGALAFTLPMGEARLPGIAADDIGKCALGIFKKREA
                     YLGKTVGIAGEHLTGSQMATALGKALGREVRYNAVPPEVYRNFGFPGADDLANMFQFK
                     RDFNAVFCAAREPAIARALNGGLQTFAQWLEVNKARIPLS"
     gene            complement(6440..6658)
                     /locus_tag="PLS35_13540"
     CDS             complement(6440..6658)
                     /locus_tag="PLS35_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19741.1"
                     /translation="MRYSLYFAWVLAVLSICILTTLASFPVLTNGERHPEPRITAGPE
                     KLRTIKIHRLRCNPFTSSCQLSRQKSQH"
     gene            6878..8368
                     /locus_tag="PLS35_13545"
     CDS             6878..8368
                     /locus_tag="PLS35_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNI19742.1"
                     /translation="MLLSFVILYLACSVGIGLYAATRVHNTKDFAVAGRSLPLPVVTA
                     TVFATWFGAETVLGISATFVKDGLRAVVADPFGSSLCLILAGLWFARRLYRLNLLTIG
                     DFYRLRYNRAVEVLCTLCIVASYLGWVSAQIKALGLVFNVVTDGAMSQSAGMMLGAVI
                     VLTYTTFGGMFSVAILDFVQITIIMGGMLYIGSVISGLAGGVGTVVTHAAAAGKLDFF
                     PPAQIEAWIPFVGAWVTMMFGSIPQQDVFQRITSARNEDTAVRGSVLGGTLYFVFAFV
                     PMFLAYSATLIDPAQVAGLLERDSQLILPTLIVQHTPMAAQIIFFGALLSAIMSCSSA
                     TLLAPSVAFSENIVKSLMPTISDRGLLAVMRSVLVGFTALVLLFALNSEASIFKMVES
                     AYKVTLVAAFVPLLAGLYWPRATTQGAICAIVTGLATWLMLEGTVTPDQVWPPQLVGA
                     LMAAGGMVIGSLLPQWITHPATIDVPVGHRSHSMPVESHTGTHPSP"
     gene            complement(8365..>10687)
                     /locus_tag="PLS35_13550"
     CDS             complement(8365..>10687)
                     /locus_tag="PLS35_13550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19743.1"
                     /translation="QSQQVSVLYRNMPTGVLASAVNAAILAAVEWSVVPHTPLLVWVA
                     ILWIVAGARALLIYNYRRTTPATWSSADWHRWMLVATTMAGVGWGGSVYFLTPEIPLA
                     YELVQLFVLGGMTAGAVSVLSVSFPLFLCYALPVTLPTLGHLFFSGYDFHVIMGVMGT
                     LFLLATIHSSWTFNHVLLSSLRLQLEKLSLVQSLTTRTEAVERLNRDITKEIHDRRVI
                     EEQLRTAQSDLEHRIAERTADLAHANSRLQDEVREHRRTEGARLSSEKRFNYLTDNLN
                     QGVWFAQAQPPQVLYVNPAFERIWGLPAARFYENPKLWRDCVHPDDQRMVTEVYDTEL
                     TRPTGRDVQLLYRIIRPDGQTRWIHDRMVIHRTETGEVDSLSGITEDITDARELEDQL
                     RQAQKMEAVGRLAGGVAHDFNNVMTVILGYSAVLLQELSQNSSARYFVQEIQRAGERC
                     AALTGQLLAFSRKQMLHPVSLDLHRVIRDLMALLKSLIGEHVTIVLQLDPSPRWVKAD
                     AVQLEQVLLNLAVNARDAMPHGGTLTIDTCQVLPHEVWGPGHDSRTTRTYVRLRVHDT
                     GTGIDPATKAKIFEPFFTTKPPGRGTGLGLSTVYGIVHQSGGTISVDSAVGQGTTMTV
                     FLPEVVPPHIALPPAPAESDQKSGTEIILLVEDEPSVRLLTQHILRTHGYTVHEAEDG
                     FQAMDLIRRNNLHIDLLITDLVMPGMNGKELAMRLRSHFDDLKVLYMSGYSDNLPVTG
                     GESQGQTTFLQKPFSPEDLIRLVREILHSVSPA"
BASE COUNT         2050 a         2909 c         3409 g         2319 t
ORIGIN      
        1 gtggtcgcgg accgcggcat ccacaatggc ctgaagcgtc gcctggtcga acggtgtggc
       61 tagataatag taggccccgg cctgcagtcc ctccagggtc tgctgatgtg tcgaggcggc
      121 agtctgcatg atgacgggaa tctggttcag tgcatcatgt tgcttcatct tgtggaggac
      181 ttccatccca tccatcccgg gcatgatgcg gtcgaggagc acggcgtgga agcgatcggg
      241 ctcagcttcc agcatatccc aggcttgccg gccgtcttgg gctgtggtgc agcgatattg
      301 acgttcggga agaaactctc tcagaatttt gaggttcaga gtcgagtcat cgaccagtaa
      361 cagttgcggt tgagggaaaa tcataatatg gagcacctcc gcttggcggc caagccgtcg
      421 gaagaaaaag catagagtct ggcctcacat cgcagaacaa ggccgtgata tgtctatcgg
      481 aagtctctga agaatactta agtgaagaag gaaggaatat cgcgatggac tccgccgggg
      541 tctaacccca ggcggagtca tcagagaggt ctattgcttc acgatatcaa gcagttccac
      601 ttcaaacacc agggtggcgc ctggcttgat caccgggggt gacccacggt cgccgtaggc
      661 gatgccggac gggcagacca gcttgctctt gccgccgacc ttaatcaact gtaccgcttc
      721 ggtccaacac ttgatgacct ggttgagcgg aaacgtggcc ggttctccac gcttgacgga
      781 gctgtcgaag acggttccat cggtcagggt gccatggtaa tggactttga ccgtatccgt
      841 ggctttgggg gtcgcgcctt ttccttcctt gatcgtggtc acaatggcgc cggattctgt
      901 ctttttggca cctggctccg ctgcggcctt ggccaggaat gcggctccgg cttttttctc
      961 gacatcggcc agggcattgg accggacctg ctgcatctgc tgaatcttgg gcccgtacac
     1021 gttcatgtcc accttctgtg accgcttcag caccccatcg gcgatgcccg acttgacgaa
     1081 ctcaagttcg gcttcgttca acgtaaaggg cgtgagtgat tggctgatgg cgacaccgag
     1141 ggcatacagc gttttttgct cgtcgttggc cggttcggag gcggccgccc aactgagcga
     1201 ggagatgaga agcaccactg cggaaacggc accagtcaca tgacgcatag acgatccttt
     1261 ctgctgaaac agcatgactg agcccaccat atgacaggca gcgtatgcgg ctcaagataa
     1321 ttcttgggca ggcggctgaa aacgacctcc cgcgtcgttc tcggctcgtc aaaatcctca
     1381 acgtacccct gcgggtacgc ctccggtttt gactcgcctg cggcctagcg agacgtcgtt
     1441 ttgagcagcc tgcgaaaatc gcgcgggctc gtttgaggcc gtgcctgctg aaagatttgt
     1501 ccgaggcaca ggatggggtc gacgtcgctt gattgagcag gttgtcgtca agtgaggctc
     1561 aggagaactt gtctcgtccg tgagggctct caagatccag ttgcggtccg atgggcacga
     1621 tctgggtcgg gttgatgtca tcatgcgtga catagtaatg ccgcttgata tgatccaaat
     1681 tcaccgtgtc cgcgatgccg ggtacctgat agaggtcttt gaggtagccg gagagatggg
     1741 gatagtcgat gatgcgccgg acattgcact tgaagtgtcc gtgatagacg gcgtcgaagc
     1801 ggatgagggt gacaaacagt cgccagtcag attccaggaa ttgcgggccg aagagatagc
     1861 ggcgttggct gagtctggtg tccaattgat ccagggcagc aaagagcgac cgcgccgctt
     1921 gttcgtaccc tcgctgagaa gtcgcgaatc ctgccttgta gaccccattg ttcacctgct
     1981 catacaggaa tgtgttcatc tcatcgatgt ctgcgcgcag cgctgcagga tagagatcca
     2041 gcgtactgat ggtgaagcgg ttgaactccg cgttgaacat ccgcatgaga tcgtcatccg
     2101 aattgctcac gattcgcttc gtgacggtat cccacaggac cgggacagtg acgcggcttc
     2161 tgtaggtggg gtcggtcagg tgataggcct cgctgaggaa gtgaaatcca ttgatgggat
     2221 cgatggagtg gccctggccg gtacgaaacg cccagccctg ttcgtcgcga atgggatcca
     2281 ccacggtcat gccgacgacc gactccaact gtttgagtgc gcggacaatg atcgtgcgat
     2341 gggcccaggg acaggcaagg gacacataga gatgatagcg cccggctgcg gccggcaacc
     2401 ctgatcgtcc gtcgtcagtc acccattggc gaaaggcgtc ttcttgccgt tgaaattcgc
     2461 ctgactcggt gtgttcgttt ggaaactgcg gctgtggggt catgcttgat tctccttgag
     2521 cgttcgacct tagtcagatt cgatgagtgc cgtcaatccg ggatcgtcaa gagacgtgag
     2581 tcgcacggca gcgacggttt attttcgacc aggcggggaa acctggtggg gcgctgaaag
     2641 atcccatccg accgaaaaga gcacgagaag cccggcgccg gtcgtgtgct tggtacgggc
     2701 gctggtctcc cgcgggggca acggctcgtt ggcgacaagc ccgtctccca gaacctgcgt
     2761 atccagatcc aggatgccgc cgataccgaa attgaaactg tactgatcgc tgcgatgcgg
     2821 ccggtagccc accatgagcc cgcctcccac ggcgtcgatg atccgggatc ccggagccac
     2881 ggccagaaat ggtccccaac tccatcgttg ctcatcccag cgatcgatcc agaggtgaac
     2941 ttcgggcatc gcgcgaacca ggtcgttgcg ttcgtcgtcg atgcgtacga tgcggttggt
     3001 gacggaaacg gattcgactc gccgcgcgcc gagattatga gtccatccca gaccgagccc
     3061 aaatcccacg ttctgaaaga gcgatgggtg tggagggtct tccgccactg gctcaggctc
     3121 ctcctgacgg ccgtagaggc tcggtgacga ctgccgaagc gtgggcgaac tgcctgtctc
     3181 cgtggcttgg gatggaggag tctgaggaag ggcgggatgg tcaggtttgt tttcaactgg
     3241 tgatgtggat agggcgagag cagccggcgc agatgtcccc tcacgctctg tcggctggga
     3301 gacgcgctcg cgatgatctc tgacggatgg aggcgacacg attgtgacgg cagagtctgc
     3361 ggttgtcacc gttggcgacg gatcggttcg tcctgtgctg tcactgtcac tctggcgctt
     3421 ctcaggcggg actgatgagg agagaggctg aatcggttcc gggatggtcg ttggaggttt
     3481 gtccgaaacc tgttgggtgg ctggctgcaa tgcatgtgga gggggcgact cacggtttgg
     3541 tggcacgggc ggctccgcgg cggtcggttc tgcggccggt atcgagtgag tatcagttga
     3601 aggtgggttt ggcgacgcaa gtggtgccgt agaggttctg accggcgctg atgtctctcc
     3661 agggtgcccg cctgctacgg gcagagatac gccacccgca tcagcgctcg cgtccggcct
     3721 gtgctctggt aaacgggtgg agggggatcc agccggagtg ggcgcctcgg gtgaactctg
     3781 tgccggtagc gtgacagccg gcgcctccct gggctgatgc ggaggcaagg agaatacgct
     3841 gacctcagcg ggactgagaa ccgcgctcga attggggtgg ggaggtgctg ctgtgagggg
     3901 ctgacttgaa tctccagatt cctctccagc tgcccggcga ggtgttggcg tgtcggtagc
     3961 cggtgtcgtg tcaacagatg ccgtcctgaa cgcctgttgg agggtggcat tatcactctc
     4021 cttggccaac acgaccggag ttctcccatt ggtgtcctga ctttgaggat cggctccgga
     4081 gcgaagcagc cggtggatca cggcggtttg tcgagacaat ctatccgcat gggattcccc
     4141 gttcatctcc cagaagcgtc ggaccgcttg gtggagcggc gtgaatccca gttcgttacg
     4201 gatggtggtc tgcgcacctg cggtcaggag tgcttcgacg catggcaaat gactttggaa
     4261 gctggcttcg tgaagggggg tgtttccgtc atggtcttgt gccgctactt gcgccccttg
     4321 gctcaagagt aaaaggacga gcggaaggtt gcctcttccc gctgcggtaa tgagcggcgt
     4381 ggctccttta gcgttgcgag cttcgatatt ggctccgcgg tcgagcagca cctgggtcct
     4441 ggttgtcgaa ctttcggtga gagcggtatg aatggactgt tctgagccaa cttgtgctgc
     4501 gactggtttc gcccgtttct cgacggggcg agcatcactg acggacgagt atccgacgac
     4561 caggagaaga atggcgctgg caacgccggt gctgtggtgc aagcgaccca cgggtaccca
     4621 aaaaagacga gtcggaagat tgcaatgcgg actgtatggt ttagcataca gctcagtcct
     4681 gcgtcatccg ccgttgtgag gtgaatgaga agaagggaag atcgaaccac agcgaagtgg
     4741 atgaacccgt aggtgcacca cggctgcggg tcgagagtgg cgagacggcc taccagcggc
     4801 gatgccaccg tccgtaatag ggacggtaat aacgcggtcc ccaggcatag gggcgtgcgt
     4861 aaaacggaga gccgtaaatc caggggcgcc cgagatacag gccgaagccg acggacgggt
     4921 atccgtacag caatcctggc gcgattccgg gggacacgta tccataagtc gatggggcgg
     4981 acgattgttg agacatctgc cgctgcaatt cagccgtcat ggcgttttgc cggtcggctt
     5041 gatctttcaa ctgtccgacg gtgctttgct ggagctggac ttccgcttcc agtttggaga
     5101 ttttttcgcg ctggatttca atgaaggcct gcaggcatcg agtgtgcgca tcacccgtgt
     5161 agttggagca ttcccaggga ccggatgatt ctgcagcaaa caccggaagg gcaaacaaca
     5221 ggacactgca tcccattgcg agagaggtcc accattgccg aggggattcc aatcggtgtg
     5281 tcgtaagacg taccataacg ctcctccctg gctagctatt cacagcctat catggctgtg
     5341 ccggtgaaga ccagatccgg ctcgtagaga gtgaaacgga cgactgcctc gttggttgac
     5401 agagaggtgc gggaaagtgt gcttgcccgt gaccacgaga gacgcaaaag gagaacagaa
     5461 catgggagaa aagcaggtaa ttgcggtggt gggagcgact ggcatgcaag gtggcggatt
     5521 ggtgcgggcg atattggcca atccagccag cgggctggcg gtgcgcgcgt tgaccaggga
     5581 tgtcaattca gacaaggcca aagaacttgc ccggctcggc gcggaagtgg tggctgccga
     5641 tgtgcatgac gtcgaaagcc tgaagcgagc cttttcgggt gccgccggtg cgttttgcgt
     5701 cacgtttttc tgggcgcatt tgtctcccga aaaggagtac gccgaggccg aagccatggc
     5761 caaagccgcc aaggcagcgg gggtgcggca tgtgatctgg tcgactctcg aagatacgcg
     5821 tcgatgggtt ccgctctcag acaaccggat gccgacgttg atgggcaagt ataaggtgcc
     5881 gcatttcgat gccaaggggg aagcggatca gctgttcaag cagcttggtc tgcccacgac
     5941 cttcctactc acctcctttt actgggacaa cctcattcat ttcggcatgg gtccgaagcc
     6001 tggtccggat ggagcgctgg catttaccct ccccatgggt gaagcccgat tgcctgggat
     6061 tgcggctgac gacatcggaa agtgtgcgtt gggaattttc aagaagcggg aggcctatct
     6121 cggtaagact gtcggtatcg caggcgaaca tctcaccggc tcacaaatgg ccacggcatt
     6181 aggtaaggcg ctcgggcgcg aggtgcgcta taacgcggtg ccgccggaag tgtaccggaa
     6241 cttcgggttt ccgggggcgg acgacctagc caatatgttt cagtttaaac gcgacttcaa
     6301 tgcggtcttt tgcgcagccc gtgaaccggc gattgcccgg gcgctcaatg gcggattgca
     6361 gacatttgcg cagtggctgg aggtcaacaa ggcacgcatt cccctctcgt aacgaaggga
     6421 cggtgaccgg cccgaaaact tagtgctgag acttctgccg ggacagttgg caggaggagg
     6481 tgaacggatt gcaccgcagc cggtgaatct tgatcgtccg caacttctcc ggtcctgccg
     6541 taatccgtgg ctccggatga cgctcgccgt tcgtgagtac cggaaacgag gccagtgtcg
     6601 tcaggataca gatggacagg acggccagca cccaagcgaa gtagagtgag taacgcatgg
     6661 cctattcgta tcatggtggg attggaagtc aatgaaatgg tcgtttttcg ccatcgctgt
     6721 tcgttgatga tagttgcatg acgctgtcgt ccatacgatt tcgccagcgc tgtaggccgt
     6781 gctccttccc gccattgcgc aagtggcgtg ctgtcgctac aatcccctcg cttgccgcca
     6841 ccggcggatg tcgtctcacc gacaggctca tagccgcatg ctcctctcct tcgtgatttt
     6901 gtatcttgcc tgctcggtcg ggatcgggct gtatgccgcg actcgcgttc ataacaccaa
     6961 ggactttgcc gtcgctggcc gctctctgcc tctaccggtg gtaacggcca cggtctttgc
     7021 gacctggttc ggggcggaaa ccgtgctcgg catttccgcc accttcgtca aggacgggct
     7081 ccgtgcagtc gtcgccgatc cattcggatc gagtctctgc ttgattctgg ccggcttgtg
     7141 gtttgcccgc cgactctatc gattaaatct cctcacgatc ggagattttt accggctgcg
     7201 gtacaaccgc gcagttgaag tgctgtgcac gctctgcatt gtcgcttcgt atctcgggtg
     7261 ggtttcggcc cagatcaagg cattggggct tgtattcaac gtcgtcacgg atggagccat
     7321 gagccagtct gccggcatga tgttgggggc agtgatcgta ttgacgtaca cgacgttcgg
     7381 tggaatgttc tcagtggcga ttcttgattt cgtgcagatc accatcatca tgggcggcat
     7441 gctctacatc gggtctgtaa tcagcggcct ggcgggtgga gtggggacgg tcgtcactca
     7501 tgcagcggcg gcgggcaaat tggacttttt cccgcctgca cagatcgagg cgtggattcc
     7561 gttcgtaggc gcctgggtca ccatgatgtt cggatccatt cctcagcagg atgtgtttca
     7621 gcggattacg tcggccagga atgaggacac cgcggtgcgg ggatccgtac tcggcggaac
     7681 gctgtatttt gtcttcgcct tcgtcccgat gtttctggcc tattcagcga cactgatcga
     7741 tccggcgcag gtggccggtt tgctggagcg ggattcccaa ctcattctcc cgactctgat
     7801 cgtgcaacac acgccgatgg cggcacagat tatttttttc ggcgctttgc tgtccgcgat
     7861 catgagttgt tcatcggcca cgctgctcgc cccgtcagtg gccttcagcg agaatatcgt
     7921 caagagctta atgccaacca tcagcgatcg gggcctgctg gccgtgatgc gctcggtgct
     7981 ggtcggattt actgcactcg tcttgctgtt cgcgctgaac tcggaagcca gcatttttaa
     8041 gatggtggaa agcgcgtaca aagtgacgct cgtggcagcc tttgtgccgc tcctggcggg
     8101 cttgtattgg ccgcgcgcga ccacgcaagg agcgatctgc gccattgtca ccgggctcgc
     8161 gacgtggctg atgttggaag ggacagtcac gccggatcag gtctggccgc cgcagttagt
     8221 cggagcctta atggccgcag gtggaatggt gattggatcg ctgctgcctc agtggattac
     8281 acatccggcg accatcgacg tcccggtcgg gcatcggtct cacagtatgc cggtggagtc
     8341 gcacaccggg actcatccgt cgccctaggc tggggagacg gagtgcagga tctcgcgcac
     8401 gaggcggatc aagtcttccg gtgagaatgg cttctgcaaa aaggttgtct gtccctgact
     8461 ctcgccgccc gtaaccggca ggttgtcgct gtaccccgac atatagagca ccttcaaatc
     8521 gtcgaagtgg cttcgcagcc gcatggcgag ctccttgccg ttcatgcccg gcatcaccag
     8581 gtcagtgatc aatagatcga tgtgcagatt attgcgccgg atgagatcca tcgcctggaa
     8641 cccgtcctcc gcttcatgca cggtatagcc atgcgtgcga agaatgtgct gagtcagcaa
     8701 gcggaccgac ggctcgtctt ccacgagaag aataatttct gtccctgact tctggtctga
     8761 ttcggcgggc gcgggtggca gggcgatatg cggcggtacg acttccggaa gaaacaccgt
     8821 catcgtggtg ccctgtccca cggcgctatc gacggagatt gtccctccac tttgatgcac
     8881 gatgccgtag accgtggaga ggccgagtcc ggttccccga ccgggcggtt tggtcgtaaa
     8941 aaacggctcg aagattttgg ccttggtggc cgggtcgatg ccggttcccg tgtcgtggac
     9001 gcgcagccgg acataggtgc gagttgtgcg ggagtcatgt cctggtcccc acacctcgtg
     9061 tggcagcacc tgacaggtat cgatggtcaa ggtgccgcca tgcggcatcg cgtcgcgagc
     9121 gttgacggcc aggttgagca gcacctgttc gagttgcacg gcgtcggcct tgacccatcg
     9181 cggactgggg tcgagctgga gcacgatcgt gacgtgttcg ccgatcaggc tcttgagcag
     9241 tgccataaga tcgcggatca cgcgatgcag atccaacgag accgggtgca acatctgctt
     9301 gcggctgaag gccagcagtt ggccggtcag cgccgcgcag cgttcgccgg cgcgctgaat
     9361 ttcctgcacg aagtaccggg ccgaagaatt ctggctcagt tcctgaagaa ggactgcgct
     9421 gtagccgaga atgacggtca tgacgttgtt gaagtcgtgc gccacgcctc cggccaatcg
     9481 accgaccgct tccatttttt gcgcctgccg caattggtct tccagttcgc gagcatccgt
     9541 aatatcttcc gtaatgccgc tcagactgtc cacctcgccc gtctcggtgc gatgaatcac
     9601 catgcgatcg tgaatccacc gggtttgccc gtcgggtcgt atgatgcgat acagcaattg
     9661 cacgtcccgt ccggtgggcc gggtcagttc cgtgtcatac acctcggtca ccatccgttg
     9721 gtcgtccgga tgcacgcaat cgcgccaaag tttggggttc tcataaaatc tggcggccgg
     9781 cagtccccag attcgctcga aggccgggtt cacgtaaagg acttgaggcg gctgcgcttg
     9841 ggcaaaccac acgccctggt tcagattgtc ggtgaggtaa ttgaaccgtt tttcgctcga
     9901 gagccgggcc ccttccgtcc gccgatgttc ccgtacctca tcctgcagcc ggctgttggc
     9961 atgggccagg tcggccgtgc gttcggcgat gcgatgttca aggtcgctct gggccgttcg
    10021 taattgctct tcgatcacgc ggcgatcatg tatttccttg gtgatgtctc ggtttaaacg
    10081 ctccacggct tcggtgcgcg tggtgagcga ctgcaccagg ctaagctttt ccagctgcag
    10141 ccgcagggag gagagcagca catggttgaa ggtccaggac gagtggatcg tagccaggag
    10201 aaacagcgtc cccatcacgc ccatgatcac atgaaagtca taaccgctga agaagagatg
    10261 cccgagtgtg ggaagggtga ccggcagcgc gtagcataag aagagtggga acgagacgga
    10321 caggaccgag accgctccgg ccgtcatgcc accgagcacg aacaactgca ccaattcgta
    10381 ggcgaggggg atttccggcg tcagaaaata gacactgcct ccccagccga cgccggccat
    10441 ggtggtcgcg accagcatcc agcgatgcca gtcggcgctg ctccacgtgg caggagtcgt
    10501 tcgacgatag ttgtagatga ggagcgccct ggcaccggca actatccaga ggatggctac
    10561 ccagacgagg agtggcgtgt gcgggaccac gctccattcc actgcggcaa gaatggcggc
    10621 gttgacagcg ctcgccagca cgccggtggg catgttgcgg tacagaacag agacttgttg
    10681 tgattgg
//
LOCUS       DAOQEW010000132        10680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1900_length_10680_cov_7.583906, whole genome shotgun sequence.
ACCESSION   DAOQEW010000132 DAOQEW010000000
VERSION     DAOQEW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_10680_cov_7.583906"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1606)
                     /gene="treZ"
                     /locus_tag="PLS35_13555"
     CDS             complement(<1..>1606)
                     /gene="treZ"
                     /locus_tag="PLS35_13555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNI19744.1"
                     /translation="YPDPCSRFQPEGPHGPSLIVDPGAYTWHDDRWPGVAMHGQVIYE
                     LHIGTFTPEGTFEAAIERLDGLKEIGITVIEVMPVAEFPGRWNWGYDGVGLYASAHVY
                     GDANSFKRFVDEAHRRGLGVILDVVYNHLGPDGNYLPTFSAEYFTDRYPNEWGQAINF
                     DGPGSGPVRDFFIQNACYWIREYHVDGLRLDAVHAFHDASPVHVVAELSQAARQAAGP
                     RSIILVAECEAQWVQTIQPLAQGGWGLDGVWSEDFHHTVRVAATGRSEGYYNDYRGTP
                     QEFISCVKRAFLYQGQRYQWQGKPRGTVVSEEPAEGFVFFLQNHDQIANQLWGDRLHQ
                     KVSPSVYRALTALLLLAPETPLLFMGQEFAASSPFLFFTDFPPGQLANDIHQGRKQFL
                     SQFPSYASSAAQDAIGDPSAPAMFERSKLDWTERERHAGIVALHRDLLRLRREDDVIA
                     QQRRDRVDGAVIGPSAFLLRYAGQHGDNRLLLVNLGPDLDFRPAPEPLLAPPSGRMWT
                     FRWSSDAPSYGGPGVIEPLTETGWKIP"
     assembly_gap    1607..1706
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1782..2642)
                     /locus_tag="PLS35_13560"
     CDS             complement(1782..2642)
                     /locus_tag="PLS35_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNI19745.1"
                     /translation="MPRSLWKGSISFGLVNIPVVLYSAENRNSFDLTLLDRRDMKPVG
                     FKRYNKESGKEVTWDQIVKGYEYDKNRYVVLTDEDFKRANVEATQTIDIVNFVRAEEI
                     APTSFETPYYLAPDKHGEKGYALLRETLKKTGKAAIATVVIRTRQYIAALIPWSEMMV
                     LNTLRYANEIRSSKELDLPAKHEKSAQVTPREVDMAMKLVEEMSEPWKPERYKDTYHE
                     DLMRLIEKRIKAGKTEVVTTPGKEEGGEKPGRAKVVDLMALLKRSVQQSGHSKHPARE
                     KERSPRRKSA"
     gene            3035..4588
                     /locus_tag="PLS35_13565"
     CDS             3035..4588
                     /locus_tag="PLS35_13565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI19746.1"
                     /translation="MHDEPGRTVSIWMSEDIPQLPPISTGAHADVCVVGAGIAGITTA
                     YCLMKEGRSVILLDDSQPGSGMTERTTAHLSNAIDSGYLDIERLHGRSGARLAAESHT
                     AAIDWIEKTIKDARIDCDFLRVDGYLCAASPDDAQHIKDEWMAVLRAGLSEVEHITRP
                     AGPLAKNGPCLRYPHQGQFHPLKYLAGLVKALQSGGCRAFSGAHVTKVESGKQARVET
                     SQGCVVTADAVVVATNTPINNMVTIHTKQAAYITYVIGAPIPAEAIEQVLWWDIQDPY
                     HYVRTQRHSLPDGREELLLIVGGEDHKAGQADDAERRYGSLETWAREYFPAMGAVSYR
                     WSGQVMESVDGLAFIGRNPGDADNIYIATGDCGMGMTHGTIAGLLLTDLIMGRECPWA
                     SLYNPSRQPVKAMGEFVRESLNVVAQYTDWVTKGDVEREEDIANDQGAVLRDGLTKIA
                     AYRDADGTLHTCSAVCTHLNCIVSWNHSEHTWDCPCHGSRFDRFGKVLNGPAITDLAP
                     VKRKERTPT"
     gene            complement(4595..5035)
                     /locus_tag="PLS35_13570"
     CDS             complement(4595..5035)
                     /locus_tag="PLS35_13570"
                     /inference="COORDINATES: protein motif:HMM:NF016045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HNI19747.1"
                     /translation="MVSKHTKTRDSGLFVRFANEASRVVGSAGAFGVAIVVVLLWSLS
                     GPLFGYSDTWQLVINTGTTIVTFLMVFLIQNTQNRDSAATQLKLDELIRSTQGAHNAL
                     LDLEKLSSKDLEDIRMLYQKLADRARREKKGGWEDTDTPELKRV"
     gene            complement(5058..6038)
                     /locus_tag="PLS35_13575"
     CDS             complement(5058..6038)
                     /locus_tag="PLS35_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19748.1"
                     /translation="MSYSGRIHLIAGWCGLVLIVGGCTTVLPPADHGAANESSQRCVP
                     AMTKRARVAAAGDETPVTPTTEARSRFSSDALQVAEVVGVLPLVNHLASDTGSSRRDP
                     LASVVLRQRLTEILLLTLFEITSTTAELTCERDRADQVADRIDEIDGARVKRLTIASI
                     LFGGIASIVSGGIGLAAGASVGGEAADVAGGALASWFGVSALFTHSEVEFRHERNVLQ
                     EIWEDPSQPRLFSPTIWRYLHRAHDEKGKLPREQVLDSWHQAGRMGENGSAAEQERRR
                     LFFGAGGRYGAAELHARASMLETLEASIRLLYEELEVLMRELAEETADVS"
     gene            6186..7916
                     /locus_tag="PLS35_13580"
     CDS             6186..7916
                     /locus_tag="PLS35_13580"
                     /inference="COORDINATES: protein motif:HMM:NF016972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNI19749.1"
                     /translation="MRYMALAADYDGTLASDGAVYPETVAALERLVASGRKLILVTGR
                     ILPELLDIFPQISLCELVVAENGGVLYRPATKEVTLLADAPRPAFLEEVQRRAVQHLS
                     LGHVIVATRVPYETVILDIIRDLGLELRIIFNKGAVMVLPHGINKATGLTAALARLEL
                     SPHNTVAIGDGENDHAMLALSEYGVAVANAVPMLKETADRTTAGDHGGGVIEIIDHLL
                     LDDLVAAEADVSRHGLLLGRRPSGEEVTLPFGRRNLLVAGTSGSGKSTLATGLLERLG
                     EQGYQLCVIDPEGDYDNVPQAVILGTAQNGPSLTEILTALANPQTHVVVNLVGLPLHD
                     RPAFFFGLLHKILELRAASGRPHWILIDETHHLLPRDGNPGAPGLTQDCTGMMYVTVH
                     PDQIDPAILKTVDLVFALGQSPGKTIEAFCQARGQQAPTLPSTELEAGQTLMWDPTLA
                     VAPEVLEIAPSSTERRRHRRKYAEGELPPDRSFYFRGPAGKLNLRAQNLLLFMQLGEG
                     VDEQTWQYHLNAHDYSTWISDMIKDETLSKVVHDIEARPELSAEESRRLIRAAIEEQY
                     TVPATGIEPA"
     gene            complement(8198..9079)
                     /locus_tag="PLS35_13585"
     CDS             complement(8198..9079)
                     /locus_tag="PLS35_13585"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HNI19750.1"
                     /translation="MSPSEIIRKSGGSAFHRLTGLLLTLVLLLSGCALSFGYRHADWL
                     ITRQLDHYLDLTSSQRRIVNARLQPLLWRHRIEALPQYEQFLKDLHARIGRGLTSEDV
                     EWTFASYDRFRADLFERAIPEGTQLAMLLKNNQITYLERVFRKEEVQAESLARRSVST
                     RLDERARKGIATAEEWLGPLSHEQSVQLREMMLALPDSQPTWWQYRRQRHGEVLAMLR
                     TSTSADQIAERLRYLFVQSDQTAPTGYLKMMTEMRAALTRMVLDVDRMLTPAQRRHVL
                     VAIQKVIDEVHALAHNS"
     gene            9286..9723
                     /locus_tag="PLS35_13590"
     CDS             9286..9723
                     /locus_tag="PLS35_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19751.1"
                     /translation="MKTLMHSLLALALVSLAACSSSPTASKTRSEMDVGTKTGMVKNI
                     VIKDTLTPDALTVRAGDEVRWINQRQDSVTVTIDGPLEHNVSCRNGFSKALGMGVDNS
                     TTLASNETAGLCFSRVGTVNYSVRPSSDTRTTMADTKGSITVQ"
     gene            complement(9838..>10680)
                     /locus_tag="PLS35_13595"
     CDS             complement(9838..>10680)
                     /locus_tag="PLS35_13595"
                     /inference="COORDINATES: protein motif:HMM:NF036542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HNI19752.1"
                     /translation="LAAHGEEGGPRESPQGFTTAPGREDREEQGDKLRVRPELFADHY
                     SQARMFWKSLTDNEQAHLASAFVFELAKVNLDEVQSRMVGRLRNVDEGLAKRIADGLG
                     MPLPGKIRAAREPIEMKPSPALSIQKNMLATLKGRKVGLLYAEGSNGGDIDRMIKQVE
                     KAGGTVFLVAPKLHGTKLENGKELKADGQLAASPSQLFDAVAIILSAKGAQMLLKEGA
                     AIQFVMDAFGHLKAIGHSEAAQPLLDKAGVVPDEGVTDLRGSFVEAAAKRFFAREPSV
                     RTLA"
BASE COUNT         2210 a         3248 c         2941 g         2181 t
ORIGIN      
        1 cgggaatttt ccagccggtt tcggtcaacg gctcgatgac tcccggtcct ccgtaactcg
       61 gggcatcgct ggaccagcga aacgtccaca tacgcccgct tggcggcgca aggagcggct
      121 ccggagcagg acgaaaatcc aagtcgggcc ccagattcac gagcagcaag cgattatcgc
      181 catgctgacc ggcataccgg aggagaaacg ccgaaggccc gatgacggcc ccatccaccc
      241 gatcacgccg ctgttgtgcg atgacatcgt cttcccgacg aagacgcaac aggtctcgat
      301 gcaacgccac gattccggcg tggcgctccc gttccgtcca atccaatttc gagcgctcga
      361 acatggccgg cgcgctgggg tcaccgattg cgtcctgcgc agcactcgac gcataactgg
      421 gaaactgaga caggaattgt tttcgtcctt ggtgaatgtc gttcgctaat tgccccgggg
      481 ggaaatcggt aaaaaataaa aacggcgagg aggctgcgaa ttcctgtccc atgaacaaca
      541 agggcgtctc cggcgccaag agcaggagag ccgtcagagc gcgatacacg gatggactga
      601 ctttctgatg taagcgatcg ccccacaact gattcgcgat ctgatcgtgg ttctgcagaa
      661 aaaacacgaa cccttcggcc ggctcttccg acaccaccgt gccccgtggc ttcccttgcc
      721 actgatatcg ctgcccctgg tagagaaacg cccgtttcac acaggaaatg aattcttgcg
      781 gcgtgcctcg ataatcgttg tagtagccct cgctgcgccc ggttgccgcc acgcgcaccg
      841 tatggtggaa gtcttcactc catacgccgt cgagccccca gcctccctgt gccaacggct
      901 gaatggtctg gacccactgc gcttcacatt cggcgaccag gatgatggaa cggggaccgg
      961 ccgcttgcct ggctgcttga gacaattccg cgacgacatg cacggggctg gcatcatgaa
     1021 acgcatggac cgcgtctaag cgcaatccat cgacgtggta ttcgcggatc cagtagcagg
     1081 cgttctgaat aaaaaaatca cgcaccggcc ctgaaccggg gccgtcgaaa ttgatcgcct
     1141 gcccccattc attcggatac cgatcggtga aatactcagc gctgaatgtc ggcaagtaat
     1201 tcccgtcggg ccccaggtgg ttgtacacca catccaatat gacgccgagt ccacgccggt
     1261 gcgcttcgtc cacgaagcgc ttgaagctgt tcgcgtcgcc atacacatgg gccgaggcat
     1321 acaggccgac gccatcgtag ccccagttcc agcgacccgg aaattccgcc acaggcatga
     1381 cttcgatgac agtaatgccg atttctttca aaccgtcgag ccgctcgatg gcagcttcga
     1441 atgtgccttc aggggtgaag gtaccgatgt gaagttcgta gatcacctgc ccatgcatgg
     1501 cgacgccggg ccaccggtca tcatgccagg tgtatgcgcc ggggtcgaca atcagtgatg
     1561 ggccgtgggg tccctcaggc tggaaacgcg agcaggggtc cggatannnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnntctc cgcttccggc tccgctttgc tgaccccagc
     1741 catacctagc cctccactgt ctacagccaa tctgagtgac ctcaggcgct cttacgccgt
     1801 ggcgaccgct ctttctcacg agcagggtgt ttggaatgac cggactgttg aacactgcgc
     1861 ttgagaagag ccatcaaatc gaccaccttg gcgcgaccgg gcttctcccc gccttcctct
     1921 ttcccgggtg tcgtgaccac ttccgtttta ccggccttga tgcgcttttc gatcaaccgc
     1981 atcaaatctt catgataggt atctttgtag cgctcgggtt tccacggttc ggacatttcc
     2041 tcgacgagct tcatggccat gtccacttcg cgcggtgtca cctgtgccga cttttcatgt
     2101 ttggcgggca gatcgagctc cttgctggag cggatttcgt tggcataccg cagcgtattc
     2161 aacaccatca tctcgctcca gggaatcaat gccgcaatgt attgccgcgt acggatcact
     2221 acggtggcga tggccgcctt accggtcttt ttcaacgtct cgcgcaacag cgcatagccc
     2281 ttctcaccat gcttatcggg ggcgagataa tagggcgtct caaacgacgt gggggcaatc
     2341 tcttctgccc gcacgaaatt gacgatgtcg atcgtttgtg tcgcttccac attggcgcgc
     2401 ttgaaatcct catcggtgag cacgacatac cggtttttgt cgtactcata tcctttgacg
     2461 atttgatccc acgtgacttc tttcccgctc tccttgttgt agcgtttaaa cccgaccggc
     2521 ttcatgtcgc gccggtctaa cagggtaagg tcaaaactgt tccggttttc ggccgagtag
     2581 agcaccaccg gaatattgac caggccgaaa ctgatcgatc ctttccagag ggagcgtggc
     2641 atggggcacc tatttgttag gggtaggccg aggcggcgag gcctgcatac aagtgacgca
     2701 cacatgcgca cagttcacgc acagcaccgg ctctccgcac tcgtcgcaga cgagcaacat
     2761 cgtggcctcg acaagctggc acccatccac ccggcaggtt ggatcggcgc agaagtgaca
     2821 atcgtatgga cagaccatca atcgatgaat gctggttctc gtgagagcga ccctgttcaa
     2881 gggcaaatca tttgaagatc gaatcatctc ttcactccta cgacccgaca actaggccca
     2941 tccctagctg ttatccctgg ggcagcccga taggcttggc caatattcat gaggccaccg
     3001 tcagcctccc gcgcaccatc gtaaggagtc cgccatgcac gatgaacctg gacgcacggt
     3061 ttccatctgg atgtccgaag acatccctca acttccacct atatccacag gcgcgcatgc
     3121 cgatgtgtgt gtagtcggcg ccggaattgc cggcatcaca acggcctatt gtctgatgaa
     3181 ggaaggccgt tctgtgatcc tcttggatga cagccaaccg ggaagcggca tgacggaacg
     3241 aacgacggca cacctctcga acgccatcga ttccggatat ctggacattg aacgtttgca
     3301 cggtcgatca ggggcccgcc tggctgcgga aagtcatacg gccgccatcg actggatcga
     3361 aaagaccatc aaagacgcca ggatcgactg cgactttctt cgggtggacg gatatctctg
     3421 cgctgcctct ccggacgacg cgcagcacat caaagacgaa tggatggccg ttttgcgggc
     3481 aggcctcagc gaagtcgaac atatcacgag accagccggc cccctggcca agaacggtcc
     3541 ctgcctgcga tacccgcatc aggggcagtt tcacccgctc aaatacctgg ccggccttgt
     3601 caaagcgctc cagtcaggag gatgccgggc attcagcggc gcgcatgtca ccaaggtgga
     3661 aagcggcaaa caggcccggg tggaaacgag tcaaggatgc gtcgtcacgg cggatgccgt
     3721 cgtcgtcgcc accaatacgc ccatcaataa catggtgacg attcatacca agcaggcggc
     3781 ctatatcacc tatgtcatcg gagcgccgat tcccgctgaa gccattgaac aagtgttgtg
     3841 gtgggatatt caggatccct atcactacgt gcggacgcag cggcattcgt tacccgacgg
     3901 gcgtgaggaa ctccttctta tcgtcggcgg cgaagaccat aaggccggtc aggccgacga
     3961 tgcggagagg cgctacggca gtctggaaac ctgggcccgc gagtatttcc ctgccatggg
     4021 ggcggtctcc tatcgatggt cggggcaagt aatggaatcg gtcgatggcc tggcgtttat
     4081 cggcagaaat ccgggcgacg ccgacaatat ctacattgcg accggtgact gcgggatggg
     4141 tatgacccac ggaaccattg ccggtctgct cctgaccgac ctcatcatgg gccgggaatg
     4201 cccttgggca tcgctctaca atccttcccg ccagcccgtg aaggcgatgg gagaatttgt
     4261 gcgcgagtcg ctcaatgtgg tggcgcaata taccgattgg gtgacgaaag gggatgtcga
     4321 acgggaggag gatattgcca acgatcaggg cgcagtactg agagacggcc tcacgaaaat
     4381 tgcggcctac cgtgatgccg atggcacgct gcacacctgt tcggctgttt gtactcacct
     4441 caattgcatc gtctcctgga accattccga gcacacctgg gattgcccct gtcatggatc
     4501 gcgcttcgat cggttcggga aggtcctgaa tggacctgcc attactgatc tcgcgccggt
     4561 caagcgaaag gaacgtacgc cgacttgatc ggcctcacac ccgcttgagc tcgggcgtat
     4621 ccgtgtcttc ccacccgcct ttcttttcgc ggcgggcccg gtccgccaac ttctgatata
     4681 gcatccggat gtcttcgagg tctttcgagc tcagcttttc caggtcgagc aacgcgttgt
     4741 gggcaccttg tgtcgagcgg atcagttcat caagtttcag ctgcgtggcg gcgctatccc
     4801 ggttttgggt attctgaatc agaaacacca tgaggaaggt gacaatggtc gtccccgtgt
     4861 tgatgaccag ttgccacgta tcactgtaac cgaacagcgg ccctgacagg ctccacagca
     4921 acaccacgac aatcgccacg ccgaacgcgc cggcgctgcc caccacccgc gaagcctcat
     4981 tggcaaaccg cacgaagagt cctgaatccc gcgttttcgt gtgtttggaa accatggtct
     5041 gactcctctg gtgacattca tgagacgtcg gcggtctctt cggccagttc acgcatcagc
     5101 acttccaact cttcatagag caaccggatc gaggcttcca gcgtctccag catagacgcg
     5161 cgcgcatgta attccgccgc cccgtaccgg ccgcctgcgc cgaaaaataa gcggcgccgt
     5221 tcctgctcgg cggctgaccc gttttctccc atcctgcctg cctgatgcca tgagtcaaga
     5281 acctgttccc tcggaagctt tcccttttcg tcatgcgctc gatgcaagta ccgccagatg
     5341 gtgggagaaa agaggcgcgg ttgcgaggga tcttcccaga tttcctggag cacgttgcgc
     5401 tcatgacgaa attccacctc agaatgcgtg aataaggctg acaccccgaa ccaggacgcc
     5461 agagcccctc cggccacatc ggcagcctcc ccgcccacgc tagcgccagc agccaacccg
     5521 atcccccccg agacgatgct ggcaattccc ccgaagagga tcgaggcgat cgtcaaccgc
     5581 ttcacccggg ctccatcgat ttcatcgatg cgatccgcca cttgatcggc tcgatcacgt
     5641 tcgcaggtga gttcggccgt cgtcgaggtg atctcaaaga gggtcagcag aaggatttcc
     5701 gtgagccgct gacggagaac gacggatgcc agaggatccc gccgcgatga tccggtgtca
     5761 ctcgcaaggt gattgaccag ggggaggaca cccacgacct ccgcgacctg caaggcatct
     5821 gaactgaatc tggagcgggc ttccgtggtg ggtgtcacag gcgtttcatc cccggccgca
     5881 gccactcgcg cccgcttcgt catcgcaggc acgcagcgtt ggctcgactc gttggcggca
     5941 ccatgatcgg ctggcggaag cacggtggtg catccaccga cgatcagcac aagcccgcac
     6001 caccccgcga tgagatgaat ccttccactg taactcattg gatattcgct catctttttc
     6061 tggcatgaaa gaagatgccc gccgactcgg aaactcccta ggctccttcc tagctccacc
     6121 gactgatgga agcttgtata catcgcactg ggtcacgggt aaacgattgg gggaaggacg
     6181 aaccgatgcg ttatatggcc ttggcagcag attacgacgg caccctggcg tcagacggag
     6241 ccgtgtaccc cgaaaccgtc gccgccctcg aacggctcgt cgcttccggc cgcaaactca
     6301 ttctcgtcac gggtcgaatc ctgccggagc tgctggacat ttttccgcag atttcgttgt
     6361 gcgaattagt ggtggcagag aacggcggtg tgttgtaccg ccccgccaca aaagaggtga
     6421 ctcttctggc cgacgcccca cgaccggcat ttctcgaaga ggtccaacgt cgcgcagtcc
     6481 agcatctcag cctcggacat gtcattgtcg ccacccgtgt cccctacgaa accgtgatcc
     6541 tcgacatcat ccgcgatctt ggactggaac ttcgcatcat tttcaataag ggcgccgtca
     6601 tggtgctgcc gcatggcatc aacaaagcca ccgggttgac ggcggcgctg gcccgtctgg
     6661 agctgtctcc tcataacacg gtcgccatcg gcgatgggga aaacgaccat gccatgctgg
     6721 cgttatctga atacggcgtg gcggtggcca atgccgttcc tatgctcaag gagacagcgg
     6781 atcgcaccac tgccggtgac catggaggcg gggtcatcga aatcatcgac catttgctct
     6841 tggacgatct agtcgcggca gaagcggacg tgtcgaggca cgggctgcta ttgggacgtc
     6901 gacccagcgg tgaagaggtg acgctcccct tcgggcggcg gaatcttctt gtggcgggca
     6961 cgtccgggag cggaaagtct acccttgcca ccgggctctt ggagcggctc ggcgagcaag
     7021 ggtaccaact ctgcgtcatc gatcctgaag gggattatga caatgtcccg caggcggtga
     7081 tcctgggcac cgcccagaat ggtcccagcc tcaccgaaat cctgacagct ctcgcaaacc
     7141 cgcagacaca tgtcgtggtg aacctcgtcg ggcttccgtt acatgaccgg ccggcgtttt
     7201 tcttcggtct gctccacaag attctggaat tgcgcgcggc aagcggtcgc ccgcattgga
     7261 tcttgatcga tgaaacgcac catcttctcc cgcgcgacgg caatcccggc gccccgggcc
     7321 tgacgcaaga ctgcacgggc atgatgtatg tgaccgtaca ccccgatcag attgacccgg
     7381 ccattctcaa aacagtggat ctggtgttcg ccctcgggca atcaccgggg aagaccatcg
     7441 aggccttttg ccaagcaaga ggacagcaag ctccaacctt gccctcaaca gaactcgaag
     7501 ccggtcagac tctcatgtgg gatccaacgt tagcagtcgc gcctgaagtc ctcgaaattg
     7561 cccccagcag cactgaacgc cggagacata gacgcaagta tgcggaaggc gaacttccgc
     7621 cggaccgcag tttttatttt agaggtccgg cggggaaatt gaatttgcgg gcgcaaaacc
     7681 tcttgctctt tatgcagctc ggcgagggcg tcgatgaaca aacctggcag taccatctta
     7741 acgcccatga ctattccacg tggatcagcg acatgatcaa ggatgaaacc ctctccaagg
     7801 tggtccacga cattgaagcc cgaccggagc tctcagccga ggaaagccgg cggctcattc
     7861 gcgcggccat tgaagaacag tacacggtac ctgccacagg gatcgaacct gcgtaatacc
     7921 cgcagcccag catctcgttc ccaagggctt cacctagtac cgcatcacac tgttccattg
     7981 catcgtgaca atagcagcgc acatttccat ccgtccggga atgtccgctc tgtcggaggg
     8041 cgaggggtag cgaacccagt tcggtgcccc tcccttccga tcatttcaac gagtgtgatg
     8101 acgaaggaga gagactatgc cgattatatt atggctgctt ggcgtgccgc tgagtttgat
     8161 tctgttgctg tggcttttcg gggtggtcca cttctagtta cgaattgtgc gctaatgcat
     8221 gtacctcgtc gatcaccttc tgaatcgcga ccagcacatg acgtcgctga gccggtgtca
     8281 gcatgcgatc cacgtccagg accatacgcg tcagtgccgc gcgcatttcc gtcatcatct
     8341 tgaggtaacc ggtcggggcc gtctgatcgg attgaacaaa caggtatcgt aaccgctccg
     8401 caatctgatc ggcggaggtg gaagtgcgga gcatggccag cacctcacca tgccgctgtc
     8461 gacggtactg ccaccacgtc ggttgactgt caggcagagc caacatcatc tcgcgaagct
     8521 gaacggattg ctcgtggctc agaggtccaa gccattcttc agccgtcgcg atcccttttc
     8581 tcgcacgttc atcaaggcgc gtagaaactg atcgtcgcgc cagactctcc gcttgaacct
     8641 cctccttgcg aaacacccgt tccagatagg tgatctggtt attcttcagg agcatcgcca
     8701 gctgcgtccc ttcgggaatg gcccgctcaa acagatctgc ccgaaaccga tcgtaggagg
     8761 caaaagtcca ttctacgtct tcggaggtca accccctgcc gatgcgggcg tgaagatctt
     8821 tgagaaactg ttcgtactgc ggtaacgctt cgatgcggtg ccgccagagc aatggctgca
     8881 ggcgggcgtt gacgatgcgc cgctggctcg acgttaagtc caagtaatga tccagttgcc
     8941 tcgtgatgag ccagtcagca tgccgatacc cgaaggagag ggcacagccg ctcaggagca
     9001 agacgagcgt gagtagcagg cccgtcagcc gatggaacgc tgaacctccg ctttttcgaa
     9061 tgatttcaga tggactcatg ccccctcacc cattcagtct ttcttcatga ataaccatcc
     9121 ggccgcctga cgcatactgg ggctttcccg agtacggagt cgtcatcgtg ctgagtactg
     9181 tgggcatgga ggcctcgcac tcccacagag gacacatggc ctatcgcatc agcggcacgt
     9241 cgcggtgatg tcgcgatcat ttcatcttac caaggaggat tacccatgaa gacattgatg
     9301 cactcactac ttgccctcgc gctggtgtcg ttggccgcct gctcgagttc accaaccgcc
     9361 tcgaagaccc ggtcagaaat ggacgtaggc accaaaacag gcatggtgaa aaatatcgtg
     9421 atcaaagata cgctcactcc cgatgccttg acggtgcggg cgggcgacga ggttcgctgg
     9481 atcaatcagc gacaggattc cgtgacggtc acaattgacg gccccctgga gcacaatgtg
     9541 tcctgccgca acggattctc caaggcgcta gggatgggcg tcgataattc caccacgttg
     9601 gcgagcaatg aaacggccgg actctgtttc agccgggtcg gcaccgtgaa ctactcggtc
     9661 aggccttcga gcgatacgcg aacaacgatg gctgacacga aaggttccat cacggttcag
     9721 taaagcagaa acggctgctg gtgtgcgatg tctgtcgata gaggcatcgc acaccagccc
     9781 gggctttcac tccccccata cctgtccgtg gttccactcc aacgctgagg atgcctgcta
     9841 cgccaacgta cgcacggacg gttcgcgagc aaaaaagcgc ttggcggctg cctccacgaa
     9901 tgaccctcgc agatcggtca cgccttcatc cggtaccaca ccggctttgt ccaacagagg
     9961 ctgcgccgcc tccgaatggc caatcgcctt gagatgcccg aacgcatcca tgacgaattg
    10021 aatcgccgcc ccctctttga gcaacatctg cgcgcctttg gccgagagga tgatggccac
    10081 agcatcgaat aactgagacg gtgaggcagc cagttgcccg tcggccttca attccttgcc
    10141 gttctccagt ttcgtcccgt ggagcttggg cgcaacaaga aagacggtcc cgccggcctt
    10201 ctcgacctgt ttgatcattc gatcgatgtc cccgccgttc gatccttccg catagagcag
    10261 gccgaccttg cgtcccttca acgtcgccag catatttttc tggatcgaca gagcagggct
    10321 cggcttcatc tcgatcggct ccctggccgc gcgaatcttc ccagggagcg gcatgccaag
    10381 accatccgcg atccgcttcg ccagcccctc atccacgttc cgcagcctgc cgaccatacg
    10441 cgactgaacc tcgtcgaggt tgacctttgc cagctcgaag acaaacgctg atgcgagatg
    10501 ggcctgctca ttgtccgtga gggatttcca gaacatgcgg gcctgactgt aatgatcggc
    10561 aaagagttcg ggacgaacgc ggagtttatc gccctgttcc tcccgatctt cacggcccgg
    10621 tgcggtggtg aatccctgcg ggctttccct gggaccacct tcctccccat gggcagccag
//
LOCUS       DAOQEW010000133        10386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1995_length_10386_cov_7.998451, whole genome shotgun sequence.
ACCESSION   DAOQEW010000133 DAOQEW010000000
VERSION     DAOQEW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1995_length_10386_cov_7.998451"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PLS35_13600"
     CDS             complement(<1..1294)
                     /locus_tag="PLS35_13600"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HNI19753.1"
                     /translation="MAGKQDMKTNQGHGGETHQQAGGPKEPVLTTDQGIPISDNQNSL
                     KSGARGPTLLEDFVLREKIFHFDHERIPERIVHARGSAAHGFFEVYESLSSLTSADLF
                     QRKGERTPVFVRFSTVVGGAGSVDTPRDVRGFAVKFYTKTGNWDLVGNNIPVFFIQDA
                     MKFPDLVHAVKMEADSGFPQAASAHDTFWDWASLMPESTHMLMWAMSDRTLPRSFRMM
                     EGFGVHTFKLVNDKGKETFVKFHWKPKLGLQSTVWEETLKLQAADNDYHRRDLREAID
                     AGNFPEWELGIQTFDQAFADKQPYDVLDATKLIPEEQIPVRRIGRLVLDRNPDNHFAE
                     NEQVAFCPANIVPGIDFSNDPLLQGRLFSYLDTQKSRLGTANFHQLPINAPKCPMANF
                     QRDGQMQMRVPTGRANYEPNSLAAHGEEGGPRESPQGFT"
     gene            complement(1410..2483)
                     /locus_tag="PLS35_13605"
     CDS             complement(1410..2483)
                     /locus_tag="PLS35_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNI19754.1"
                     /translation="MAHTWKTLLQPQSLWKLLQEAFTAWSDDKIPRHGAALAYYTVLS
                     LVPLLIVMISLIGLIFGREAAQKHILDQTTNLMGPQSGDAVKQMIEHATQPTTGTIAM
                     LIGVATLLLGASGVFAQLQDSLNTIWRVEPKEGRGLWGLIRDRFISISAVLGTGFLLL
                     VSLVLSAALAAFGKWYSGWLPAPEVVLQLLEIVISLLVIAGLFALMFKVLPDARIAWN
                     DVWVGALLTAFLFTIGKFAIGLYLGKSDVGSAYGSAGSLVIMLVWVYYSAQIVLFGAE
                     FTQVYANELGSRIVPTENAVPADEPRAGRMRPAPTQPRLQAASGRQAQAASAQRQPAE
                     RLPWWGTVLLLWVAVTRFPRRSE"
     gene            2836..4476
                     /locus_tag="PLS35_13610"
     CDS             2836..4476
                     /locus_tag="PLS35_13610"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNI19755.1"
                     /translation="MSIRIDSRQTTYTGESLHAASSVKKHHRGMWRWVMAACAAVILL
                     GGIGLWFVDEPLRVYAERQLNGRIAGYTFHIGTLDFHPIGLSVDLENVTVIQEAHPDP
                     PLAHIAEWHASIQWEGLLSGRLVSDHIIDRPLIHFTRPQAAQELQDAPAQKKSWQEAL
                     FAVYPLQINELSIKNGDVTYRENATSNPIRLTNIQVLAENIRNVRSKPLQYPSRVQVE
                     AMVAGGGHMKVDGHADFFAEPSMGVDVDITLQDINLADLLPLTAQRQIHLTQGSLTTS
                     GHVEFAPAAQEVRIASLNLHDVKVDFVHAARTKQREQRTAQQVSRTAAKAANHPTLLL
                     RIDQGKVERSEFGFVNQATNPSYRLFISGTELELENWSNQLSEGTARVQLKGMLMGTG
                     ATKITGAFRPEVESPDFDLSVKIVKTQVKSLNQLLRAYGGLDAAAGLFSLFSEVNVKN
                     GTVKGYLKPLFKDVTAYDPQQDRDKGMLNQLYEKTINALAEILKNTPRDEVATKTDLS
                     GTVENPQASTWELVLTLFQNAFFDAVLPGLEGKVQLER"
     gene            4619..5077
                     /locus_tag="PLS35_13615"
     CDS             4619..5077
                     /locus_tag="PLS35_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19756.1"
                     /translation="MKTEMKKFIPICCVCENVRGETLSPGTEPEWGPLKTYLDTHHLT
                     IGGYRLTHTYCPACAVQFTKLGRTARPAVTEEQPEIDNSVTPTILDAFDHMQQCDMDM
                     LVEACPTLTWNQVFSEVDRLSRMGQLRLSHLGYGRYSIERPQPVEVGVGS"
     gene            complement(5029..5799)
                     /locus_tag="PLS35_13620"
     CDS             complement(5029..5799)
                     /locus_tag="PLS35_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNI19757.1"
                     /translation="MRVQVASYNIHRGVGRDGRYNPGRILRVLQEVNADIVALQEVDF
                     PGPHPHHFLTWLAAQLGMTAVGGPVRTSAAGTYGNALLTRYPVRNVRRWDLSVGRHEP
                     RGALAADMLCQGAMIHVVTTHLGLWPTERPRQATKLLEGLHVTEEELTILMGDLNEWQ
                     LWGKSLRVLRKVFGAPVSPPTFPTAWPLLALDRIWVSPPRALFEVHTHQSRQSRTASD
                     HLPVKAEIRFESVHGTGSVMREDYEPTPTSTGCGRSML"
     gene            complement(5825..7855)
                     /locus_tag="PLS35_13625"
     CDS             complement(5825..7855)
                     /locus_tag="PLS35_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNI19758.1"
                     /translation="MMIVGWDIDTQVELVRETSASSEMPTKLGEFLVALLRRRRRLRV
                     SVLNWDYAVIYALERQWMPTAEPGWRRHRRLSYQVDDHHPVGASHHQKLVVIDDAVAF
                     VGGLDLSKSRWDTREHLSRDPRRVDAEGIVYAPFHDVQMMVSGDAASALGDLARNRWF
                     NATGRRLSAPPPRSVEEVWPPRVNPDLTECPVAIMRTQPRYEELEEQREIEQAYLSAI
                     KRARRSIYIESQYFTAQVISHALAQRLSEHDGPDVVLVLRQHCDGWLERRTMDSLRAQ
                     LLHEVELADHYGRLRVYAPVVPGEAGAEPVAVHSKLLVVDDEFVCIGSANVSNRSMGF
                     DTECNLAVEAGGQARVQSAVAAFRNGLLAEHLGVDPEVVANQIQTCGSLAAATDALRG
                     GGRTLADDCFEAMPPSDAMIPEHALVDPERPMAADHLLAGVGFRRERSRIVRRAMAGL
                     TLLAGMAILAAAWRWTALGQLLDVEALVSYAQALGRSPLGLISVMGGYLIGGLLAVPV
                     TLLLVVTILAFGPWFGALFALTGSLLSAITLFGLGRVLGSYRIRRLAGSRVEHLSRRM
                     AERGLWAVLLVRILPIAPFSVINLIAGASSVSWRHFVLGTVVGMLPGIAIMATFVSGL
                     EEAVREPSWQAFSMVGLVVAAAWAGVWYVSSRLQSRQSTESVMAAGSSVPAT"
     gene            8211..8621
                     /locus_tag="PLS35_13630"
     CDS             8211..8621
                     /locus_tag="PLS35_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HNI19759.1"
                     /translation="MRMSTSIQSVDQHRLDCIQACLDCARICNTCGNDMIGMDMSEDE
                     EAQDLMVRCIRLCRDCADICLLAAQWMGRTSAFAVRLCAFCVEMCELCAGVCEQHAPH
                     HAMCGDCAKECRRCAELCHKMVLESVPASTGGTA"
     gene            8644..9111
                     /locus_tag="PLS35_13635"
     CDS             8644..9111
                     /locus_tag="PLS35_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19760.1"
                     /translation="MMLNTLMGSGLLTVVLLTGGQAVQQDVPAPVPNPGPGPTVPPKP
                     SDPIPTPPTVPKLLLQAWSGVERTSLFLIAEAAPRSQEAVSGKLLHLDLEKKEGRLGT
                     DLGREVPFRIPKPELFVHLSVGQRVTIKLDSGQQAVGVMETAAPELPSPSPAR"
     gene            9344..>10386
                     /locus_tag="PLS35_13640"
     CDS             9344..>10386
                     /locus_tag="PLS35_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNI19761.1"
                     /translation="MKATDMFSWLTGSEMGGRIRAFDWSRTPLGPIEHWPASLVSILG
                     VCLTAQTPMAIYWGADSWLLYNDAWRPVVGGKHPWALGHPAREVWPELWPTIQHYFHS
                     VLNTGEANWRTDELLPMQRFGYTEECYFDYSLNPIRGQSGAVEGILNIVQETTYRVLN
                     DRRTSLLRELASLSGSAQNDTDACARILQALATDSADVPFALLYQIDRDHREAQLAGS
                     IGLEDASLARQEQLDLTGNAAPGSWPLAAAIRNGGPLLLDELPDGFGLLPGGAWPEPT
                     RQCLLLPIGTATQDGGVVLVAGISPRRPLDEHYHHFFRMVSSHIAVAINNARAYQSEQ
                     RRAEALAELDRAKT"
BASE COUNT         2213 a         3226 c         2922 g         2025 t
ORIGIN      
        1 tggtgaatcc ctgcgggctt tccctgggac caccttcctc cccatgggca gccagactat
       61 tcggctcgta gttggcccgg ccggtcggca ctcgcatctg catctggccg tcgcgctgaa
      121 agttggccat tggacacttc ggcgcgttga tcggcagttg gtgaaaattg gcggtcccca
      181 accgggactt ctgcgtatcg agatacgaaa acaaccgtcc ttgcagcagc ggatcgttcg
      241 aaaaatcaat gccgggcacg atgttggcgg gacaaaaggc gacctgttcg ttttccgcga
      301 agtgattgtc aggattgcgg tccagcacca ggcgcccgat gcgtcggaca ggaatctgtt
      361 cttccggaat cagtttggtc gcatcgagga cgtcatacgg ctgtttatcc gcaaacgcct
      421 gatcgaacgt ctggatgccc aattcccact ccggaaaatt ccccgcatcg atggcctccc
      481 gcaaatcacg ccggtggtaa tcgttatcgg ccgcctgcaa cttcagcgtt tcttcccaga
      541 ctgtcgattg caggccgagt ttcggtttcc agtgaaactt cacgaacgtc tctttgcctt
      601 tgtcattgac cagcttgaaa gtatgaacgc caaagccctc catcatacga aacgatcgcg
      661 gaagagtccg gtcggacatg gcccacatca acatatgcgt gctttccggc atgagcgacg
      721 cccaatccca aaaggtatcg tgcgcgctgg ccgcctgcgg gaaaccgctg tctgcctcca
      781 tcttgacggc gtggacgagg tcgggaaact tcatggcatc ttgtataaaa aagacaggaa
      841 tattattgcc gaccaggtcc caattccccg tcttggtgta gaacttgacg gcaaaaccgc
      901 gcacatcacg gggagtatcg acggagccgg cgccaccgac gacggttgaa aaccgcacga
      961 agacaggcgt ccgttcgcct ttgcgttgaa acagatcggc gctcgtcaat gatgaaagcg
     1021 attcgtacac ttcaaagaat ccatgcgcgg ccgatccccg cgcgtgcaca atgcgctccg
     1081 gtatccgctc atgatcgaaa tggaagattt tttcccgcag cacgaaatct tccagcagcg
     1141 tggggcctcg cgcaccgctc ttgaggctgt tttgattgtc ggaaatggga atgccctggt
     1201 cggtcgtcaa caccggctct ttgggtcccc ctgcctgctg atgcgtttct ccaccatgcc
     1261 cctgattcgt cttcatatcc tgctttccag ccatacgtgt tgttcctctc ttcatatcgt
     1321 cgtttgatgc ctgccctctc aaccgttcac ctcaatcgtt cacgcggttc tggccgctcc
     1381 tccattccat ctcggtccct gttcattgtt cattccgaac gccgtgggaa gcgggttact
     1441 gccacccata acagcaggac tgttccccac caagggaggc gctctgcagg ctgacgctga
     1501 gctgaggcag cctgtgcttg ccgaccggat gccgcctgca atcttggctg tgtcggtgct
     1561 gggcgcatgc gtccggccct tggctcatcg gccgggacgg cattctccgt cgggacgatc
     1621 cggctcccga gctcattggc atagacctgc gtgaattctg ccccgaacag gacgatctgc
     1681 gcggagtagt acacccagac caacatgatg acgagtgatc cggccgaccc ataggcggac
     1741 cccacatcgc tttttcccag atacaggcca atggcgaatt taccgatcgt aaacaggaac
     1801 gccgtcaaca gggctcctac ccacacatca ttccacgcga tgcgggcatc gggaagcacc
     1861 ttgaacatca aggcaaagag tcccgcaatc accagcaacg aaatcacgat ttccaagagt
     1921 tggagcacca cctcgggcgc cggaagccac ccgctatacc atttcccgaa ggccgccaat
     1981 gccgcactga gaaccagaga cacgagcagc aggaaccctg tacccagcac cgcgctgatc
     2041 gagataaatc gatcgcgaat gagtccccac aagccgcgcc cctctttcgg ctctactctc
     2101 cagatcgtat tcagcgagtc ctggagttgc gcgaatacgc ctgacgcgcc caacagcagc
     2161 gtggctacgc cgatcagcat ggcgatcgtt cccgtggtgg gctgcgtggc atgctcgatc
     2221 atttgcttga ccgcgtcccc gctttgcggc cccatcagat tggtggtttg atccaaaatg
     2281 tgtttctgcg ccgcttcccg cccgaagatc agtccgatga gggagatcat cacgatcagg
     2341 agaggcacca gcgacaacac ggtatagtag gccaacgcgg ccccgtgccg cggaatcttg
     2401 tcatcactcc aggccgtgaa agcttcctga agcagtttcc acaaggattg cggttgcagc
     2461 agcgtcttcc atgtgtgtgc catcttgcgg gcccctctcg acgtgaaatg atcgttcgta
     2521 atggcagggt ctagcgagat gccaatcgta aggacctgac gcttcaattc ggctgacgag
     2581 aagtagagcg atcctcacct gcttacgtcc gcagttacac tgtcacgctc cgtctgccgc
     2641 gcataactcg gcattaccct agagccgaaa agggccgatt ctcggctgca cgccttctcg
     2701 aaaccagggg ccttcccagt agccgagcag gcaaagcggc attacgatcc gagcgcaatg
     2761 tccatgtcgt gttattccgc cgacaggtcg ggggtttctt gaggtaggtg tcgttattca
     2821 gcgggtcaga cggttatgag cattcggatc gatagccgac agacgaccta cacgggtgag
     2881 agtctgcatg ccgcatcgtc tgtcaagaaa caccatcgcg gcatgtggcg ctgggtcatg
     2941 gctgcctgtg cggccgtgat tctcctgggt ggaatagggt tatggtttgt cgatgaaccc
     3001 ttgcgggtct atgccgaacg tcagttgaac ggccgtatcg cgggctacac cttccatatc
     3061 ggtacgctcg actttcatcc tattggcctg tctgtggact tggaaaacgt aaccgttatc
     3121 caggaggccc atccggatcc accactcgca cacatcgcgg aatggcatgc cagcatccag
     3181 tgggaggggc tgctatccgg ccgcctggtc agcgatcaca tcattgaccg tcctctcatt
     3241 cacttcaccc gaccccaagc ggctcaagaa ctgcaggacg cgccggctca aaagaaaagt
     3301 tggcaggagg ccctcttcgc cgtctacccg ctacaaatca atgaactcag tatcaagaac
     3361 ggcgacgtga cctatcgaga gaacgcgacg tccaatccca tacgcctgac gaacattcag
     3421 gtcctggcgg agaacattcg aaacgtgagg tcgaaaccgt tacagtaccc ctcgcgggtg
     3481 caggtcgaag cgatggtggc cggtggaggc cacatgaaag tcgatgggca tgcggacttt
     3541 tttgccgaac cgtcgatggg cgtcgatgtg gacatcacgc tgcaggacat caacctggcc
     3601 gacctgctcc ccctcaccgc tcaacgccaa atccacctca cccaaggttc attgacgact
     3661 agcggtcatg tggaatttgc ccctgcggcg caagaagtgc ggatcgcttc gctgaacctt
     3721 catgatgtga aggtggactt tgttcatgca gcccggacga aacagcgcga gcaacgtacg
     3781 gcacaacagg tgagccggac ggcggcgaaa gctgccaacc atccgacgct cctccttcgg
     3841 atagaccagg ggaaagtcga acgcagtgag ttcgggtttg taaaccaggc gacgaatcct
     3901 tcctatcgcc tgttcatcag cgggacggag ctggagcttg agaactggag caaccagctc
     3961 tccgaaggca cggcgcgggt gcaactcaag ggcatgttga tggggaccgg cgcgaccaag
     4021 attaccgggg cgttcaggcc ggaagtcgaa tccccggact ttgacctttc tgtgaagatc
     4081 gtcaaaacgc aggtgaaatc tctcaatcaa ttgttgcgcg cctacggggg tctggatgcc
     4141 gcggccggcc tgttttcgct gttcagcgag gtgaacgtca aaaacggcac cgtcaaggga
     4201 tacttaaagc ccctgtttaa ggatgtgacg gcctatgatc cgcaacagga ccgggataag
     4261 gggatgctca accagctgta cgaaaagacc atcaatgcgt tagctgaaat tctcaaaaac
     4321 accccgcggg atgaagtcgc cacgaaaact gacctgtccg gaacggtgga aaacccccaa
     4381 gcgagcacct gggaactggt gctgaccctc ttccaaaacg ccttcttcga cgcggtcctg
     4441 cccggcctcg aaggtaaagt acagcttgag cgttgagcca ggcccactcc tcgcccctcc
     4501 acatcatgac gggcgagaac taggggagca cctagtagtc ggagggcaga atattttcta
     4561 cagtgcgaca ctagttgacc ggttgcagtt cattccgaca agaagagagg ggtgggttat
     4621 gaagacagag atgaagaagt ttatccccat ctgctgcgta tgtgaaaatg tgcgaggcga
     4681 gactctgtca cccgggaccg agcccgagtg gggccctcta aagacgtatc tcgatacgca
     4741 tcacctgacc atcggagggt atcgtctcac ccacacctac tgccccgcat gcgccgtgca
     4801 atttaccaag ttggggagaa ctgctcgccc tgcagtgacc gaagagcagc ctgaaatcga
     4861 caacagcgtg acccccacca tcctcgatgc gtttgaccac atgcagcagt gcgatatgga
     4921 catgctggtg gaagcctgcc cgactctcac ctggaaccag gtattttcag aggtcgaccg
     4981 gttgagccgt atgggacagc tacgcttgag ccacctggga tatggccgct acagcattga
     5041 acggccacag cctgttgaag taggcgtcgg ttcgtagtcc tcccgcatca cagaccctgt
     5101 gccatgcacg gactcaaacc ggatctccgc cttcaccgga aggtggtcgg aggcggtgcg
     5161 actctggcgc gactgatggg tgtggacctc gaataatgcc ctgggcggag agacccaaat
     5221 ccgatccaac gccaacagag gccaggccgt gggaaaggtc ggtggcgaga cgggcgcccc
     5281 gaacaccttg cggagcaccc gcagcgactt cccccacaat tgccattcgt tcaggtcgcc
     5341 catgagaatc gtcaactctt cttctgtgac gtgtaaaccc tccagcaatt tggtggcctg
     5401 ccgaggccgc tccgtcggcc acagtcccaa gtgtgtcgtc actacgtgaa tcatcgcgcc
     5461 ctgacagagc atgtccgcag ccagggcgcc tcgtggctca tgtcttccga ctgaaagatc
     5521 ccatcgcctg acgtttctca ccggataccg cgtcaacaac gcgttgccgt aggtgcctgc
     5581 cgccgatgtt cgcaccggtc cacccacggc cgtcatacct agctgagcag ccagccacgt
     5641 caaaaagtga tgcggatgcg ggccgggaaa atccacttcc tggagcgcga caatatccgc
     5701 gttcacctcc tgtagcacgc gcagaattcg cccgggatta tagcgtccgt cccttccgac
     5761 tccgcggtga atattgtacg aggccacctg cacacgcatc gatggcaccc ctttgctgaa
     5821 ggacctacgt cgctggaacc gaggaaccgg cggccatgac actctcagtc gactgcctgg
     5881 actgcagtcg gctcgatacg taccacacgc cagcccaggc cgcggcgacg accaacccca
     5941 ccatcgagaa ggcctgccaa ctcggttccc tgacggcttc ttcgagaccg ctcacgaagg
     6001 tcgccatgat ggcgattccc ggcaacatgc ccacaacggt gcccagcaca aagtgtcgcc
     6061 atgacaccga tgaggcaccg gcaatcagat tgatgaccga gaacggtgcg atcgggagga
     6121 ttctcaccag gagcacggcc caaagccctc gttcggccat acgtcgactg aggtgttcca
     6181 cgcgggaacc agccaatcgc cggatccgat atgagcccag gacccggccg agtccaaaca
     6241 gcgtgatcgc actcaacaac gatccggtca gcgcgaacaa ggcaccgaac cagggaccaa
     6301 aggcgaggat cgtcaccacc agcagcaggg tcacagggac cgcgaggaga ccgccaatga
     6361 gatatccgcc catgacagat atgagcccca gcgggcttcg tcccagcgcc tgcgcataac
     6421 tcaccaacgc ttcaacatcc agcaattgtc ccagagccgt ccatcgccag gcggctgcga
     6481 gtatcgccat tcctgccagc aacgtgaggc ccgccatggc gcgccggacg atccgcgacc
     6541 gctcccgccg gaatcccacg ccggccagaa gatgatccgc ggccatggga cgctccggat
     6601 cgaccagcgc atgttcagga atcattgcat cggaaggagg catcgcttca aaacagtcgt
     6661 cggccagggt ccggccgccc ccgcgcaatg cgtcagtcgc cgccgcgagc gacccgcagg
     6721 tttgaatctg attggcgacc acttcgggat cgacgccaag atgttccgcc agcaatccgt
     6781 tgcgaaacgc cgcaacggcg gattgcactc gtgcctggcc gcccgcttcg accgccaggt
     6841 tgcactccgt gtcgaatccc atggaacgat tggagacatt ggcggatccg atgcagacga
     6901 attcgtcatc gacaaccaga agtttgctat gcacggcaac cggttcggcg cccgcctctc
     6961 ccggcacgac cggcgcatac acacgcagcc ggccatagtg gtcagccagt tccacttcgt
     7021 ggaggagttg ggcgcgcaaa ctgtccatcg tccggcgctc cagccacccg tcgcaatgct
     7081 gacggagcac caacaccaca tccggcccgt catgctcgga cagccgttgg gcgagcgcat
     7141 gactaatgac ctgggccgtg aaatattgcg actcgatgta aatcgatcga cgcgcgcgtt
     7201 tgatggcact tagataggcc tgttcgatct cgcgttgctc ttccagttct tcgtatcgag
     7261 gctgggtgcg catgatggcg accgggcatt ccgtcagatc ggggttcacc ctcggcggcc
     7321 acacctcttc gacggatcgt gggggcggcg ctgaaagtcg ccgtccggtc gcgttgaacc
     7381 accggttgcg cgcgaggtcc cccaaagccg acgccgcgtc gccgctgacc atcatttgca
     7441 cgtcatgaaa cggggcatag actatccctt cggcgtcgac acgacgagga tcccgggaaa
     7501 gatgctcccg cgtatcccag cgggacttac tcaaatcaag cccgccgaca aaggcgactg
     7561 catcatcgat caccacgagt ttttgatggt gcgacgcgcc caccggatgg tggtcatcga
     7621 cctggtacga gagacgtcga tgccggcgcc accccggttc ggccgtgggc atccattgcc
     7681 gctccagcgc ataaatcacc gcataatccc agttaagaac agacacccga agcctgcgtc
     7741 tgcggcgtaa cagcgccacc agaaactcgc cgagcttggt cggcatctcc gacgaggcag
     7801 aggtttcgcg taccagctcg acctgggtat cgatatccca cccgacaatc atcatggagt
     7861 gtttcgcgcg caacgccgcc tcacggaacg cctggaagta cgcctccccg tcaacgagga
     7921 acgcgattcg ttccgctggc tctatgcgcc aacaggtgtg tccagggacg agtatgggcg
     7981 aagagccttc cggctctgct gaaggagacg acggcgcccc cctttccgct gcgttgtgca
     8041 tcgcgatgtt cgccatcttg ccgcacaccc tacccaactt tcttcacgat acccagctag
     8101 tcagacccct aggtccgcgc ccatccttca acggttcgag agttttccct agtactcgcc
     8161 actatctcag taggataggc tgaacactgg cacaaagagg agggagggcg atgaggatgt
     8221 ccacatcgat tcaatcagta gatcagcatc gacttgattg catacaggct tgtttggatt
     8281 gcgcgcgtat ctgcaacacc tgtggtaacg atatgatcgg catggacatg tcggaggacg
     8341 aagaggccca ggacctcatg gtgcgttgca tccgtctttg ccgcgattgc gcggacatct
     8401 gcctccttgc ggcgcagtgg atgggacgga cctcggcctt tgccgttcgc ctttgcgcgt
     8461 tctgtgtcga aatgtgtgaa ctctgtgccg gcgtctgcga gcaacatgcg ccacaccatg
     8521 ccatgtgcgg agactgcgcc aaagaatgcc ggcggtgtgc ggaactctgt cacaaaatgg
     8581 tgttggaatc cgtgcctgca tcgaccggcg gaaccgccta gctggccccg gcgaacggag
     8641 aatatgatgc tgaatacctt gatgggaagc ggtctgctca cagtcgtcct actgacagga
     8701 gggcaggcgg ttcaacaaga cgtccctgcg cctgtcccta atcccgggcc gggacccacg
     8761 gtgccaccga aacccagcga cccgattccc acgcctccga cggtaccgaa gctgttgcta
     8821 caggcatggt cgggagtcga gcggacctct ctcttcctga ttgccgaagc ggcacctcgt
     8881 tcacaggaag ccgtttctgg gaaattgctg cacctcgatc tcgagaaaaa agagggccga
     8941 ttaggaacag atctcggccg ggaagtcccg tttcgcattc caaaacctga actgtttgtt
     9001 cacctttcag tcggccagcg ggtcaccatc aagctggatt ccggccaaca agccgtgggc
     9061 gtcatggaaa cggccgcacc ggagcttccc tctccttctc ccgcccgata agtctctcaa
     9121 ctcatcccag aggactaggg gtttcactag tgggggctcc cgtctcattc atgctacaga
     9181 aataacgcta ggtgcagccc tcggacggac gcctgtcgcc aggttccgct ccgcatcagc
     9241 ggtgtccttc attcaagagg agtgaccccg ggcagagaat caatctctgc ccctgtatga
     9301 tcggccgacc ctcatcaacc agcagacaac tttggacggg ggaatgaagg cgaccgatat
     9361 gttcagctgg ctgaccggca gcgagatggg tggccgcatt cgggcattcg actggagccg
     9421 gacgccgcta ggccccatcg aacactggcc cgcctccctc gtatcgatcc ttggtgtctg
     9481 cctgacagcc caaaccccca tggcgatcta ttggggagcc gatagctggc tcctgtacaa
     9541 cgacgcctgg cgccctgttg tcggcggaaa acatccctgg gcgctgggcc accctgcccg
     9601 cgaagtctgg ccggaattgt ggccgaccat ccagcattac tttcatagcg tgctcaacac
     9661 aggagaagcg aattggcgga ctgatgagct gctgccgatg caacggttcg gctacacgga
     9721 ggaatgttat ttcgactaca gcctcaaccc gattcgcgga caaagcggag ccgttgaagg
     9781 cattctgaat atcgtccagg aaacgacgta ccgggtgctg aacgaccggc gaacgagtct
     9841 gctgcgtgag ctggcatcgc tgtcgggatc agcgcaaaac gacaccgacg cctgcgccag
     9901 gattctccaa gccctcgcca cggactccgc cgatgtgccc ttcgccctgc tctaccagat
     9961 cgatcgggat catcgagagg cgcagttagc cggcagcata gggctggagg atgccagcct
    10021 ggccaggcaa gaacaactgg acctgaccgg caatgcggcg ccgggaagtt ggcctcttgc
    10081 cgccgccatt cgtaatggag gcccgttgct tttggacgag cttccggacg ggttcggcct
    10141 gttgcctggc ggcgcatggc ctgaaccgac gaggcaatgc ctcctgctgc ccattggcac
    10201 ggcaacccaa gacggcggtg tcgtcctggt cgcaggcatc agtccccgtc ggcctctgga
    10261 cgagcattat caccatttct tccggatggt gagctcgcat atcgccgtcg ccatcaacaa
    10321 tgccagggct tatcaatccg agcagcgccg ggccgaggcc ttggctgaat tggatcgggc
    10381 gaaaac
//
LOCUS       DAOQEW010000134        10378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_1998_length_10378_cov_8.613291, whole genome shotgun sequence.
ACCESSION   DAOQEW010000134 DAOQEW010000000
VERSION     DAOQEW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1998_length_10378_cov_8.613291"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLS35_13645"
     CDS             <1..540
                     /locus_tag="PLS35_13645"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNI19762.1"
                     /translation="HKDAKPVYDLDSVQVMQLVTGLNNGKDYAGNKLDGSTDFTIGGA
                     VTPEADPLGPMLVKFEVKVRAGAQFFQTQAIYQPEQFKKFMDAVRPFKVKVLAGILLL
                     RNAKMAEFMNANIPGVCVPQDMIDEMRAAGDKRALDAGVEIAVRTIKAVRPYCDGVHI
                     MAIKSTERLPEILTKAELG"
     gene            540..1328
                     /gene="panB"
                     /locus_tag="PLS35_13650"
     CDS             540..1328
                     /gene="panB"
                     /locus_tag="PLS35_13650"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNI19763.1"
                     /translation="MTVPDLQKCKRDGRKIAVVTAYDALFARIVEQAGIDVILVGDSL
                     GVVVQGKANTLSVTMDEMLYHTKLVAGAVQRSLVIGDMPFLSYQVNQEEAVRNAGRLL
                     QVGAHAVKLEGGAAMTDRVRAMANVGIPVIGHLGMTPQSVQQYGGYRVQGKGKDRAQQ
                     LLEDARALEEAGAAAIVLEAIPAGLAKTVSEALTIPTIGIGAGPHCDGQVLVLYDLLG
                     LFDEFVPKFVKPYGHLKVEALQALRQYKEDVEKGVFPSDSESYH"
     gene            complement(1347..1880)
                     /locus_tag="PLS35_13655"
     CDS             complement(1347..1880)
                     /locus_tag="PLS35_13655"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNI19764.1"
                     /translation="MAESDQPVLALWPRYTDRPLPAYRFIPGRTPHPRRHPQGHSFEQ
                     PDPLLRPFEPEHWKTSEDYLYGIDLYNWTYWWEAHEVFEAFWHAYGRHTPAGNFFQAL
                     IQCAAANLKRELGHEQATRNLVSRALARLQKCPVHYMGLDTEIIAEQFTQWLNQREPH
                     GHVLFALSKAGGKTRNS"
     gene            complement(1867..2685)
                     /locus_tag="PLS35_13660"
     CDS             complement(1867..2685)
                     /locus_tag="PLS35_13660"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNI19765.1"
                     /translation="MATYAIGDVQGCFSSVTRLTKTIQFDPKQDRLWFVGDLVNRGPD
                     SLKVLRYIRALGQAAITVLGNHDIFLLAVAAGLTSPRPSDTLSAILEAPDCRELMLWL
                     RQQPLLYREGDFLLVHAGLLPAWTVEQAETLASEAHNAIRGDQFLTTLRALHPSGSLQ
                     WRPDLTGPVRVATIIKVLTRLRACSDDGIMESSFSGPPDQTPDGFYPWFDVRGRRSTA
                     ATIVFGHWAAMGLRLTPNMLALDSGCVYGRQLTAVRLEDRKVFQVGCEETRGGK"
     gene            complement(2752..5655)
                     /locus_tag="PLS35_13665"
     CDS             complement(2752..5655)
                     /locus_tag="PLS35_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19766.1"
                     /translation="MIKKGIGLRDKLNLLSIGLVVLTAIGIATFVVHREITTRYHDLV
                     NTGLTTAAMIAQNSEYAVYTENQDAVRRAVGGLHALPDIAYAAVLGKERLPILEETWG
                     ANIAIPTALRTRLLAAGDALFEEVAIQQGHPAYIDILVPVVSRPAAAADPLFLESNAS
                     SPPGAVIGYIQLGLSQERLRKNLQEFVLITGLVIGVVLLVGLCMSLILTRSITKPILA
                     LVEAAGHIAEGRLDVDVAAGTHDEVDRLADAFNRMTGRLRTSQSQVQEYQQSLEDKVT
                     QRTKQLELASQEAQRLAEEAQAASRAKSQFLANMSHEIRTPMNGVLGMTELLLSTTLD
                     TRQRHLTKTIQQSGEALLAIINDILDFSKIEAGKLQLEQLDFDVQDTVENAVELFAGP
                     AQRKQIELTCQLMGPFTHALRGDPIRLRQALLNLISNALKFTAKGEINVRVYAVTETN
                     TTVTLRFEVKDSGVGIPAEAHQRIFEAFSQADGTTTRRFGGTGLGLTIVKELVALMQG
                     QIGVESQVGQGSTFWFTAVFERQPAAAPGQEQAPEQALSKKRILVVDDTPANREILYE
                     HLRTWGALPTLAASAQEALVLLGTGSNSPQPFDLAILDLHMPDMDGLELAKAIRMDPR
                     LAGLPLLMLTSVGYDAKTPGTPDLDAWVTKPVRNTLLRQALLGLLHTRHRAPVQPPAP
                     LPSASGPIPFQAAHLLLVEDTPVNREVALGMLDMLGHFAQAVENGRLAVEAVARERFD
                     LVLMDCQMPEMDGFTATATIRQQERDAADPRHVPIIALTANAMEGDRARCLAAGMDDY
                     LAKPFTMAQLSEMLTRWLTLQATAAPMAPGPSPSARQDDSPSDQTPPSAPVEIDRTAW
                     DAILALQRPGRPDILAKVLTAYLNDSRTLVGEIRTAVQTQDAVALAKAAHRLKSSSAQ
                     LGALATAAHCKELENLGRLARLDDAPSLLAQLTDAHQAACAAMTSELLQRPAA"
     gene            complement(5673..6623)
                     /locus_tag="PLS35_13670"
     CDS             complement(5673..6623)
                     /locus_tag="PLS35_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI19767.1"
                     /translation="MRRLIHLIAALGIALTLSALWLIASPDSACAESGRIVVLSGQDL
                     KPYQDVLAGLQQSLTKQGINLAVEIHAAQGAPSTANVLEDIKKNGARLVVTLGSAATQ
                     AAVREVGQVPILATMIVTADDIKSASNATAVLLDFPLDLQMQWLHRIVPAANTVGVLF
                     NPKENQTKVNHALRIAKENGLSLVTQAVDTPRALPDALENLSRHVDALWGISDSVVMT
                     PQTAEPILLSTLRNKVPLAGLSASWVKAGALYALDRDYLDIGNQCGDIAGKILGGTSA
                     SSLAPIFPRKVTYSVNLKTAGAMNLELPQDVVRGATHIFQ"
     gene            complement(6640..8742)
                     /locus_tag="PLS35_13675"
     CDS             complement(6640..8742)
                     /locus_tag="PLS35_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI19768.1"
                     /translation="MRCLARTYNWMLPTTTGCLLAATILGTPLTSLAASPIEDAPLVQ
                     TEASLDLPDLPSSGTPDGQEPVGKESTLFEEIPSVYGAAKYDQKVNEAPAAVIIITAD
                     QIKKYGYRNFAQILDSVPGLFTSNDRNYGYLGIRGFNRPGDYTSRVLLLIDNHRLNDA
                     VYDQGGAGTEMPIDVDLIDRVEIIKGPGSALYGTNAFFGVVNVITKRGRDLKGAEVSG
                     EASSFNTYRSRLSYGNKFSNGLEMLLSGSFSDSEGHRQLSYPAFSSINNGIAKDADRD
                     NLYYYTGKLSYGDFTLLGGHQWRKKNVPTASFGTVFNDNRQNTLDERTYLDLVYQHEF
                     VNQLNVKARVYYDRYYYRAHLPYDYAGTGTPPFTLNKDSTEADWWGAELTITKRLFDH
                     HKFTIGSEYRDQFRLNQANADIAPPSTYLDDKRRSSFIAAYMQDEWAITDHLVLTAGL
                     RHDQYSTFGGTTNPRAGLISTWDKTTVKLLYGQAFRAPNPYEQFYVTGSTFKANADIK
                     PERIKTYELVVEQYLGDHLRALGSLYQYNISGLISQTTDPTDGKLFYSNVDNITARGV
                     ELAMEGKWPSGLEGRLSYALQRTENDDLKQQLTNSPQHLAKANVIVPVIADKLFAGFE
                     GRYTSSRLTLAGNSASPFYILNMSLFSQRIVKGWELSGHINNLMNQKYGYPGSGEHVQ
                     DVIDQDGRTFWVKLKYRF"
     gene            complement(8888..>10378)
                     /locus_tag="PLS35_13680"
     CDS             complement(8888..>10378)
                     /locus_tag="PLS35_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI19769.1"
                     /translation="AHQRIFEAFSQADGTTTRRFGGTGLGLTIVKELVSLMQGQIGVE
                     SQVGQGSTFWFTAVFERQPATTGDPYPEHALLQRRILVVDDTAANREILDDHLRSWGA
                     IPALTASAAEALTQLRAAAARQQPFELAILDFQMPDMDGMQLAQAIRADPQLSSLPLL
                     MLTSVGYDAGAPGVPAVDGWVTKPVRKTLLQQAILGLFRTGQRAAIRRPDPVVSEPVT
                     TVSHSARLLLVEDTPVNREVATGMLDVLGYSVHAVENGRLALEAVAREQFDLILMDCQ
                     MPEMDGFTATAAIRQQERQSGGGHRIPVIALTANAMDGDRTRCLAAGMDDYLAKPFTA
                     AQLRTILTHWLTPPVADATTASMPTQTAPPRESTDESPQPTANPEIDKTAWEAIRSLQ
                     RPGRPDILAKVLTTYLNDSRILVEEMRAAVEARDPGTLAKAAHRLKSSSAQLGVLATA
                     AHCKELEHLGRLASLNDAAHLLAQLAETHQAACTTIAAELRSRSAT"
BASE COUNT         2011 a         2867 c         3318 g         2182 t
ORIGIN      
        1 cataaggacg ccaagccggt gtatgacctg gattccgtgc aagtcatgca gttggtgacc
       61 ggattgaata atggcaagga ctatgcgggc aacaagctcg acggttcgac cgactttacc
      121 atcggcgggg cggtcacgcc ggaagcagat ccgctggggc cgatgctggt caaatttgaa
      181 gtcaaggtgc gcgccggcgc gcagtttttc cagacccagg cgatttatca gccggaacaa
      241 ttcaagaagt tcatggacgc agtgcgtccg ttcaaggtga aggtgctcgc gggaattctg
      301 ctgctgcgca atgccaagat ggcggaattc atgaacgcca atatccccgg cgtctgtgtt
      361 ccgcaggaca tgatcgacga aatgcgtgcc gccggtgata agcgcgcgct cgacgcgggc
      421 gtggagattg ccgtgcgaac gatcaaggcc gtccgtccct actgcgatgg cgtccacatt
      481 atggccatca aatccaccga acgactgccg gaaattctga cgaaagctga gctcgggtga
      541 tgactgtccc ggacttgcag aagtgcaagc gcgacggacg caagattgcg gtggtgacgg
      601 cctacgatgc cctcttcgcc cgtatcgtcg aacaggcagg gatcgacgtc attctagtcg
      661 gcgattcgct gggcgtggtc gtgcaaggca aggcgaacac gctttccgtg accatggacg
      721 agatgctcta ccataccaag ctggtcgcgg gagcggtcca gcgctcactg gtcatcggcg
      781 acatgccgtt tctctcctac caggtcaacc aggaagaagc ggttcgaaat gccgggcgac
      841 tccttcaagt cggagcccat gcggtcaaac tggaaggtgg tgcggcaatg acggatcgtg
      901 tgagggcgat ggcgaacgtc ggcattccgg tgatcgggca tctcggcatg acgcctcagt
      961 cagtccagca atatggcggc tacagggtac agggaaaggg caaagatcgc gcacaacagc
     1021 tgctggagga tgcgcgcgcg ctggaagagg cgggcgcggc cgccattgtc ctcgaagcga
     1081 tcccagcggg attggcaaag acagtgagtg aggcgctcac gattcccacc atcgggatcg
     1141 gcgcagggcc gcactgcgat gggcaagtgc tggtgctcta cgatctgttg ggattgtttg
     1201 acgagtttgt gccgaagttt gtaaagccgt acggccacct caaggtcgag gcgctccagg
     1261 ctctccgtca gtacaaggag gatgtggaga aaggtgtgtt cccgtccgac tcggaaagct
     1321 accactgagt agcctcccac tggcgttcaa ctgttccgtg ttttcccgcc tgctttcgat
     1381 aacgcaaaca gaacgtgccc gtgtggctcc ctctgattca gccactgtgt gaactgttcg
     1441 gcgatgattt ccgtatccaa gcccatatag tgaacgggac atttttgtag gcgagcgagc
     1501 gcccgtgaaa caaggtttcg tgtcgcctgt tcgtgcccca gttcccgttt gagattggcc
     1561 gcggcgcatt gaatgagggc ctgaaagaaa ttcccggctg gtgtatgcct cccgtaagca
     1621 tgccagaacg cttcgaagac ttcgtgcgct tcccaccagt aggtccagtt ataaagatcg
     1681 atgccgtaca gatagtcttc ggacgttttc caatgttctg gctcgaacgg gcgcagaaga
     1741 ggatcgggct gttcgaatga atggccttgc gggtgacggc gaggatgtgg cgttcgtccg
     1801 ggaatgaagc ggtaagctgg aaggggtcgg tcggtatacc gcggccacag ggctagaacc
     1861 ggctgatcac tttccgccac gagtctcctc gcacccgact tgaaacactt tccggtcttc
     1921 cagacgcact gccgtgagct gtctgccgta cacgcagccg ctgtccagtg ccaacatgtt
     1981 tggtgtgagc cgcaagccca tggcggccca atgcccgaag acgatggttg ccgctgtgct
     2041 acgtcggccg cgcacatcga accaggggta aaagccatcc ggggtctgat cgggtggtcc
     2101 agagaaagaa gattccatga tgccgtcatc cgagcaggct ctcaaacgag tcaacacttt
     2161 gatgatggtg gctacccgaa ccgggcctgt gaggtcaggg cgccattgca acgaaccgct
     2221 gggatggagc gcgcgcaagg tcgtcagaaa ctggtctccc cgtatcgcgt tgtgcgcctc
     2281 gctggccaat gtctctgcct gttcgacagt ccaggcaggc agcagtcccg catgaaccag
     2341 caggaaatcg ccctcccgat acagcagcgg ttgctggcgt aaccacagca tcagttcacg
     2401 acagtccggc gcctccaaaa tcgcggatag ggtgtcactg ggacgaggag acgtcagccc
     2461 ggcggcaacg gcgagcagga agatatcgtg attgccaagc accgtgatgg ccgcctggcc
     2521 gagagcacgg atgtaacgca gaaccttgag cgagtcgggg ccacggttga cgagatcgcc
     2581 gacaaaccag agccggtcct gcttgggatc gaattgaatg gtcttcgtga gccgtgtgac
     2641 ggagctgaag cagccttgaa cgtcgccgat tgcataggta gccatgggtg ggcccagact
     2701 agcgcggacc ggcttggttc gcaagtcgcg aacggatccg tgagtgtcga atcaggcggc
     2761 tgggcgttgc aagagctcac tggtcattgc ggcgcaggcg gcctggtggg cgtcggtgag
     2821 ttgggccagc agactaggag catcatccag acgcgcgagg cgtcccaagt tttccagttc
     2881 tttgcaatgg gcggccgtgg ccaaggcgcc cagttgcgcg ctactggact ttaatcggtg
     2941 cgctgccttg gcaagggcga cggcgtcttg agtttgaacg gcagtgcgaa tctccccgac
     3001 gagggtgcgg gaatcgttca gataggccgt caacactttt gctaggatat cgggacggcc
     3061 cggtcgttga agcgcaagga tcgcgtccca ggctgtcctg tcaatctcca caggagcgga
     3121 cggcggggtc tgatcagacg gggagtcgtc ctggcgggcc gatggggaag ggcctggggc
     3181 catgggcgca gcagtcgcct gtagggtcag ccatcgcgtc aacatctcac tcagctgtgc
     3241 catggtgaag ggtttcgcca gataatcatc catcccggca gccaggcacc gcgcgcgatc
     3301 accttccatg gcattggcgg tgagggcaat gatgggcaca tggcgggggt ctgcggcgtc
     3361 ccgttcctgt tgtcgtatcg tggcggtggc agtaaagccg tccatttcgg gcatctgaca
     3421 atccatgaga acgagatcga accgttcccg cgcgaccgct tcgaccgcga ggcgtccgtt
     3481 ttcgacggcc tgggcgaagt ggcccagcat gtccaacatg ccgagggcga cctctcgatt
     3541 gacgggtgtg tcttcgacca ggaggagatg ggcggcctgg aacggaatcg ggccggacgc
     3601 ggaggggagc ggcgcgggcg gctgaactgg tgcacgatgc ctggtgtgaa gcaggccgag
     3661 aagggcttgg cgaagcaggg tgttccggac aggcttagtc acccaggcat ccaggtccgg
     3721 cgtaccgggg gtcttggcgt cataccccac cgatgtcagc atcaagagcg gcaagccggc
     3781 gaggcgtgga tccatgcgaa tcgccttggc caactccagg ccgtccatgt ccggcatgtg
     3841 cagatcaaga atggccaggt cgaacggctg tggcgagttg gagccggttc cgagcagtac
     3901 caatgcttct tgtgccgaag cggccaaggt tggaagcgcg ccccacgtgc ggagatgttc
     3961 atacaggatc tcgcgattag cgggcgtgtc gtcaacaacc aggatgcgtt tcttggataa
     4021 ggcttgttcg ggcgcctgtt cctgtccggg agccgctgcc ggttgtcgct cgaagaccgc
     4081 cgtaaaccag aatgtggaac cctggcccac ttgactctcc acgccgatct gcccttgcat
     4141 gagggccacg agttctttga cgatcgtgag gccgagtccc gtgccaccga agcgtctggt
     4201 ggttgtgccg tcggcctggg aaaaggcttc gaaaatgcgc tgatgcgcct cggctggaat
     4261 gccgacaccg gaatctttta cctcgaatcg aagggtgacg gtcgtgttcg tttccgtcac
     4321 cgcatagacg cgcacattga tctcgccttt ggccgtgaac ttcaaggcgt tactgatgag
     4381 attcagtaat gcctggcgaa ggcggatggg gtcgccgcgg agcgcgtgtg tgaagggccc
     4441 catcagctga caggtcagtt cgatctgttt ccgttgggca ggccccgcaa acagttccac
     4501 ggcgttttcc accgtgtctt gtacatcgaa atccaattgt tcgagctgaa gcttgcctgc
     4561 ttcgattttg gagaagtcga gaatgtcgtt gatgatcgcc agaagcgctt cgccggattg
     4621 ctgaatggtc ttggtcaggt ggcgctgacg ggtgtcgagc gtggtcgaca gcaagagctc
     4681 ggtcatgcca agaacgccgt tcatcggcgt ccggatctca tggctcatat tcgctaaaaa
     4741 ctgtgatttg gcgcggctgg ccgcctgcgc ctcttcggcg agtcgctggg cttcttgcga
     4801 cgcgagttcc aattgcttcg ttcgctgagt cactttgtct tccaagctct gctggtattc
     4861 ctgcacctga ctctgtgagg ttcgcaaccg gccggtcatc cggttgaagg cgtccgccag
     4921 ccggtccacc tcatcatggg tgcctgcagc gacatccacg tccaggcgcc cttccgcgat
     4981 gtgtccggcc gcttccacta atgccagaat gggtttcgtg atgcttctgg tcaggatcaa
     5041 cgacatgcag agtccaacca gcaacacgac tccgatgaca agtcccgtga tgagaacaaa
     5101 ttcctgaaga ttctttcgca gccgttcctg actgaggccc agctgaatgt agccgatgac
     5161 cgcgccaggg ggggaggaag cattggactc cagaaagagc ggatcggcag ccgctgccgg
     5221 ccgactcacg acgggcacta ggatatcgat atacgccgga tgcccttgct ggatggccac
     5281 ctcctcgaac agcgcgtctc ctgcggcaag tagacgtgtt ctcagcgccg tggggatagc
     5341 gatgttggcg ccccaggttt cttccaggat cggcagccgc tctttgccga gcacggcggc
     5401 ataggcgata tccggcaagg catgaagtcc tccgaccgcc cgccgcacgg catcctggtt
     5461 ttccgtatag acggcgtatt cgctgttctg cgcgatcatc gcggcggtgg tgagaccggt
     5521 gttgaccaga tcgtgatagc gggtggtgat ttcccgatgc acgacgaagg tcgcgatgcc
     5581 gattgccgtg aggacgacga gcccgatgct caggagattg agcttgtccc gtagaccgat
     5641 tccttttttt atcatcgtca tcctcgccgc agttactgaa aaatatgcgt cgcgccgcgc
     5701 acgacatcct gtggcaattc caggttcatg gcgcctgctg tcttcagatt caccgagtag
     5761 gtcactttgc gcggaaatat cggcgcaagg gacgaggcac tggtcccccc gaggattttc
     5821 ccggcgatgt ctccgcattg gtttccgatg tcgaggtagt cccgatcgag cgcatacagt
     5881 gcgcctgcct tgacccatga cgcggacaat cccgcgagcg gtaccttgtt ccgaagggtg
     5941 ctcagcagga tcggttcggc ggtttgaggg gtcatcacga ccgaatccga gatgccccag
     6001 agcgcatcga catggcgcga gaggttctcc aatgcgtcgg ggagggcgcg aggtgtgtcc
     6061 accgcttgtg tcacaagact caggccgttt tctttggcga tgcggagagc atggttcacc
     6121 ttggtctggt tttctttggg attaaagagc acgccgacgg tattggcggc ggggacgatc
     6181 cgatgcagcc actgcatttg gagatccagg ggaaagtcga gtaagacggc ggtggcgttg
     6241 gaggcagact tgatgtcatc ggccgtgacg atcatggtgg caaggatggg gacctgtccc
     6301 acttcacgca cagcggcttg tgtcgcggca cttcccagcg tcaccaccaa ccgggcgcca
     6361 ttcttcttga tgtcttcaag cacgttcgcg gtcgagggag cgccttgggc cgcatggatt
     6421 tccaccgcca gattgatgcc ttgtttggtc agcgattgtt gcagtcctgc gagtacgtcc
     6481 tgatagggtt tgaggtcctg cccgctcaac accacgatac ggccggattc agcgcaggcc
     6541 gaatcggggc tcgcaatgag ccagagcgcg ctcagggtga gcgcgatccc tagtgccgct
     6601 atgaggtgga taagtcgacg catcggtgat gccggttcct taaaagcggt acttcagctt
     6661 gacccagaat gtccggccat cctgatcgat gacatcctgc acgtgctcac ccgagccggg
     6721 atacccatat ttctgattca tgaggttatt gatatgtccg gacagctccc accctttgac
     6781 gatccgctga ctgaagagcg acatgttcag aatgtaaaag ggactggcgc tgtttccggc
     6841 gagcgtgagg cgtgaactgg tgtatcgccc ttcgaatccg gcgaagagct tgtcggctat
     6901 caccgggaca atcacgttgg ccttcgccag gtgctgaggc gagttcgtca actgttgttt
     6961 gagatcgtcg ttttcggtgc gctggagggc ataactcaac cgcccttcga ggcctgaggg
     7021 ccatttccct tccatcgcca actccacgcc gcgagcggtg atgttgtcga cgttgctata
     7081 gaacaacttt ccgtctgtcg gatccgtggt ctgactgatc aaaccggaaa tgttgtattg
     7141 atataggctg ccgagggcgc ggaggtgatc ccccaggtac tgctcgacca ctagttcata
     7201 ggttttgatg cgctcgggtt tgatgtcggc attcgccttg aacgtggatc ccgtcacgta
     7261 gaattgttcg tacggattgg gtgcccgaaa ggcctggccg tacaacaatt tcaccgtcgt
     7321 cttatcccac gtcgaaatga ggccggcacg cgggttggtg gtaccaccaa acgtactgta
     7381 ctgatcgtgg cggagaccgg cggtcaagac gagatggtcg gtgatggccc attcatcctg
     7441 catataggcc gcaataaagc tcgatcgacg tttgtcgtcg agatatgtgc tgggcggcgc
     7501 aatatcggcg ttggcctgat tcaggcgaaa ctggtctcga tactctgagc cgatggtgaa
     7561 tttatgatgg tcgaacagcc tcttggtgat cgtcagttct gcgccccacc agtcggcctc
     7621 tgtggagtct ttgttgagcg taaacggcgg tgtgccggtc ccggcatagt cgtacggaag
     7681 gtgtgcacga taataatagc ggtcgtagta gacccgcgct ttcacattga gctggttgac
     7741 gaattcgtgc tgatagacga gatcgagata ggtccgttcg tcgagcgtgt tctgtcgatt
     7801 atcattgaac accgtgccga aggaggccgt cggcacgttc tttttgcgcc actggtggcc
     7861 gcccaagagg gtgaagtctc cgtatgagag ttttccggtg taatagtaca gattgtcccg
     7921 gtccgcatct ttcgcaatcc cgttattgat tgaagaaaat gccgggtagg acaactggcg
     7981 atgcccttcg ctatccgaga aggagccgga cagaagcatc tcgaggccgt ttgagaactt
     8041 attgccgtaa ctgaggcggc ttcgatacgt attgaagctg ctggcttctc cggaaacctc
     8101 cgcacccttg aggtcgcgtc cccgcttcgt gatcacattg accacgccga aaaacgcgtt
     8161 ggtgccatac aaggccgagc cggggccttt gatgatttcg acccgatcga tcaaatccac
     8221 gtcgatcggc atttcggtgc cggccccgcc ctggtcgtag accgcatcgt tgagccggtg
     8281 attgtcgatc aacagcagga cacgagaggt atagtcgccc ggccggttga agccgcgaat
     8341 tcccaggtag ccatagtttc gatcgttgct ggtaaacagg cccggcacgc tgtcgagaat
     8401 ctgcgcaaag tttctgtagc cgtacttctt gatttggtcg gccgtgatga tgatgacggc
     8461 cgctggtgcc tcattcacct tctggtcata cttggccgcg ccatagactg agggaatctc
     8521 ctcgaagagc gtactttcct tcccgacggg ctcttgaccg tccggtgtgc ctgaggaggg
     8581 taggtcgggg agatccagcg aggcctccgt ttgcaccaag ggagcgtctt cgatcggcga
     8641 ggccgccaaa gaggtgagcg gggtccccag gatcgtggcc gctaagaggc agccggtcgt
     8701 tgtgggaagc atccagttgt aggttctggc aaggcagcgc atgtgcgact ccatggaata
     8761 ggacttatct ctccgttatc ggtcgagggt gcaggtaact tgacgattcc actgaggaaa
     8821 tggctggaag tgaatgccgg gcagcctagg aatgtgcgtg acgggaaagg gcgcgggatc
     8881 taagcggtca ggtggcggag cggcttcgaa gttcggcggc gatggtggtg caggcggctt
     8941 gatgcgtctc agccaattgg gccagcagat gcgcggcatc attgagagaa gcgaggcggc
     9001 ccaggtgttc cagctctttg cagtgggccg ctgtggccaa cacgccgagc tgggcgctgc
     9061 tggacttgag tcgatgcgcg gccttggcga gggtgccggg gtctcgagcc tctacggcag
     9121 cacgcatttc ctccacgaga atccgggagt cgttcaaata ggttgtgagg accttcgcga
     9181 gaatatccgg gcgtcccggg cgttgcagcg agcgaatcgc ctcccaggcg gtcttgtcga
     9241 tctcggggtt ggcggtaggt tgcggacttt cgtcggtcga ttcccgtggg ggggccgtct
     9301 gcgtgggcat ggaagcagtc gtggcgtcag ccaccggcgg tgtcagccaa tgcgtcagga
     9361 ttgtgcggag ttgcgccgcg gtaaagggct tggccagata atcgtccatg ccggcagcga
     9421 gacagcgggt gcgatcgccg tccatcgcgt tcgcggtcag ggcgatgact gggatgcgat
     9481 gccctccacc tgactgacgc tcctgttgcc gaatagcggc ggtggcggtg aagccgtcca
     9541 tttccggcat ttggcaatcc atcagaataa gatcgaactg ttctcgtgcc acggcctcaa
     9601 gggcgagacg cccgttctcg accgcatgca cggaatatcc caggacgtcc aacatcccgg
     9661 tagcaacctc ccggttgacc ggtgtgtcct cgacgaggag aagacgtgcc gagtgggaga
     9721 cagtcgtcac tggttccgat accaccggat ccggccggcg tattgctgca cgctgccctg
     9781 tcctgaagag gcccaggatg gcctgttgca gcaaggtttt ccgaacaggt ttggtgaccc
     9841 agccatcgac cgcgggcact ccaggagctc cggcgtcata ccccacggac gtgagcatca
     9901 agagggggag tgaggacaat tgcggatcag cccgaatcgc ctgcgccagc tgcatgccgt
     9961 ccatgtccgg catctggaaa tccagaatcg ctaactcgaa tggctgttgc ctcgcggcgg
    10021 ccgcgcggag ttgcgtcagc gcttcggcgg ccgatgcagt gagcgcaggg attgcgcccc
    10081 aggaacggag gtggtcatcc aggatctcgc ggttcgcggc ggtatcgtcc accaccagga
    10141 tccgtcgttg gagcagggca tgttccggat acggatcacc ggtagtcgca ggctgtcgct
    10201 caaagacagc ggtaaaccaa aaggtcgagc cctggcccac ctggctctcc acgccgatct
    10261 gtccctgcat gagggatacc agttctttca cgatcgtcag tccgaggcct gtgccaccga
    10321 agcggcgggt cgttgtgcca tcggcctgcg aaaaggcctc gaagatgcgc tgatgggc
//
LOCUS       DAOQEW010000135        10331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2019_length_10331_cov_8.238906, whole genome shotgun sequence.
ACCESSION   DAOQEW010000135 DAOQEW010000000
VERSION     DAOQEW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_10331_cov_8.238906"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..84)
                     /locus_tag="PLS35_13685"
     tRNA            complement(7..84)
                     /locus_tag="PLS35_13685"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Glu,seq:ctc)
     gene            253..687
                     /locus_tag="PLS35_13690"
     CDS             253..687
                     /locus_tag="PLS35_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19770.1"
                     /translation="MHGRYFGSRGCLVAGLLPALLTLAGCGASFLEGTIAPPNSSAWT
                     KCGSTTKVRVKPPKSTITVAYTEPTTGTDGKPLTNLAYTTIYYNAGDGPVIAKVVPAS
                     QKTGGAAVSQVILIPTPSQAEQEVTVCVTATDSDDREGPATP"
     gene            813..1337
                     /locus_tag="PLS35_13695"
     CDS             813..1337
                     /locus_tag="PLS35_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19771.1"
                     /translation="MELRKPDASHPSHEAESERHKHSLEIRLGSNIFRNTNGVIRVQG
                     KEQLVLELAPEQDRILLTIDLYDGSGNHVAHLRRNRWAFNDGNRFSLSTSDSPPTLFP
                     NLPWLKLTDQETGETVLEAAVAPGEKIHVATGKFYSHRGQLIEITSHFCRVGSTHTLF
                     GDVFEARGGTAVLG"
     gene            1353..1766
                     /locus_tag="PLS35_13700"
     CDS             1353..1766
                     /locus_tag="PLS35_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19772.1"
                     /translation="MTDSPNPSIQAFLVCDSVIEDSLTKKKSLIGIFTHLQAATFPFQ
                     HHQLGLYFCLTDAEGTYHLEIDLVYVNTDQLVCRASLPDIAIHDRLQIADFGINIPAL
                     LFPAPGRYEFRLRMNSRVIAQKDFHVIQLPAPPAA"
     gene            complement(1909..3039)
                     /locus_tag="PLS35_13705"
     CDS             complement(1909..3039)
                     /locus_tag="PLS35_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19773.1"
                     /translation="MLLVLLALCGLGGMDWAHAEQPASPELCRAQPYEAKMECYQQHL
                     EKVLLAEGTEGALTALEQITTQDPEALREAHPLVHHIGQQSFTRYGTAPLAMSHCRDV
                     FWSGCYHGVLQAFLSSLPAVEPEHILPLCPISETVSAYSFQRYNCLHGLGHGLTIQFR
                     YDVLKSLAFCDALPGSWDRESCYGGVFMENIVTFQQARRAQTASQHHHHEATSFLNPQ
                     DLLYPCSVLTEKYLRACYLMQTSAVLTFLNYDFAQAFTQCARVEGEHQTTCYRSLGRD
                     ISGYTLRDAQQVNALCRLGQGDQVKQCFIGAVKDFILTDANPEPGLALCRSLDGPFKR
                     DCYATVGEMVVPLFDDKNKRVQACHRGENEYVDACLATATAF"
     gene            3251..4954
                     /locus_tag="PLS35_13710"
     CDS             3251..4954
                     /locus_tag="PLS35_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNI19774.1"
                     /translation="MSTTPEPPTRSDFIREIVAADRAAGKHDGRVVTRFPPEPNGYLH
                     IGHAKSICLNFGIANEQPGGICHLRMDDTNPTTEDPEYVQAIQEDVRWLGFDWRDKMF
                     FASDYFERLYGFAVVLIKKGLAYVDSLTADEMRRYRGTLTEPGQSSPHRTRSIEENLD
                     LFSRMRAGEFPDGAHVLRAKIDMASPNINLRDPVLYRIRHVAHYRTGTQWCIYPAYDF
                     AHPLSDAIEGITHSICTLEFEDHRPLYDWVVAQCETPQRPQQIEFARLNVTFTVMSKR
                     KLLDLVERKLVNGWDDPRLPTLKGLRRRGFTPEALRAFCEAIGVAKRDAIVEMQLLEH
                     FVREDLNKRAPRVMAVLRPLRLVIENYPEGHVEQLDAINNPEDASAGTRQLPFSRVLY
                     IDQEDFKEDPPKQFFRLAPGREVRLRYAYIITCVGVVKDPTTGDITELRCTYDPETKS
                     GGAQAQRKVKATIHWVSAGHAVDAEIRLYESLMLTDPAKIPADQDWTQHLNPRSLERL
                     PSCKVEPSLASIAPGTSVQFERVGYFCADPDSAPDKLVFNRTVTLKDAWAKIEKSHTA
                     R"
     gene            5102..5437
                     /locus_tag="PLS35_13715"
     CDS             5102..5437
                     /locus_tag="PLS35_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19775.1"
                     /translation="MVTGSGSDDQQEERGSFREQLRRLETAIPGAASSVQPLLMNIHR
                     SVRRVHDQLQHIDVTPLLPRTLVSHVRCFDCGTAKMESFHTSTQGGYHLCPACFQHRL
                     HTGRAKTAH"
     gene            5564..7405
                     /locus_tag="PLS35_13720"
     CDS             5564..7405
                     /locus_tag="PLS35_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNI19776.1"
                     /translation="MAPNILLSCESISKSYGVRALFSNLTLALCEGDHVGLIGPNGSG
                     KSTLLKILAGLESPDQGTRTVRGHTRMGYVPQEPSFPAGSSIEHVLQQTLTDAGRDPH
                     EEGGLIARALSIGTFPDPEHCVDTLSGGWRKRLAITQALLLEPDVLLMDEPTNHLDVE
                     GILWLERLLKNRAKAFLVISHDRRFLESIATRMVELNRCYPEGRFEAKGRYSDFLEQR
                     DAALQVQADYQSSLANRVRREVEWLRRGPKARTTKAKGRIQSAGKLIEELDQVEARAA
                     QSSIGIDFSASGRKSKQLLVAKEVTKRFNEKPVVTNLDLLLGPGQRLGLLGPNGSGKT
                     TLLRLLAGTLEPDSGTIMRADGLRIVSFEQHRESLDQDAPLRRALAPSGGDAIVYQGR
                     SVHLASWAKRFLFRPEQLDLPVSRLSGGEQARLLIAQLMLQPADLLILDEPTNDLDIP
                     TLDVLEDSLLEFPGALVLVTHDRWLLDRVSTILLALDGTGRLEWFADYAQWESAQERA
                     AESETGPNEESTTTGDVAGTAPIRKPKKLSYREQKEWGTIEDTILKAEEQVAACQAAM
                     HDPKVMSNAAELQARSEALVAAQAEVERLYARWAELDEKRAETVQSS"
     gene            complement(7793..8353)
                     /locus_tag="PLS35_13725"
     CDS             complement(7793..8353)
                     /locus_tag="PLS35_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNI19777.1"
                     /translation="MSNDTVYISFSAEVNQATTEGLLGLCGQLVEKKVSTVYLMLSTP
                     GGTVMNGLNLYNVLRAMPFKLITHNVGNVDSIGNVLFLAGEQRYSSPHATFMFHGVGF
                     DINGAARFEEKTLRERMDSIQADQARIAKILCERTGLTPDEAKQLFLEAVTKDPDYAR
                     TKGIIHDVRELKLPAGAPFLQLVFKR"
     gene            complement(8445..8521)
                     /locus_tag="PLS35_13730"
     tRNA            complement(8445..8521)
                     /locus_tag="PLS35_13730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8485..8487),aa:Ser,seq:gct)
     gene            complement(8557..9108)
                     /locus_tag="PLS35_13735"
     CDS             complement(8557..9108)
                     /locus_tag="PLS35_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19778.1"
                     /translation="MGDLEQAIEERKEKVRRLEKEVDQAVELLRQAQHELEILTTARQ
                     IVTKQALLAQWEPDSPQLPLNGPVSPPQSMFHGPKTIHDGTWDYWCERYNKQYTVGEY
                     KALKREMTLVDEVKATLCNSPAPLGPAEIRGELAKTGRVVPQNVMTGVLSRLAKENRI
                     VREGRGKYRLPTDEEVQIQKETA"
     gene            complement(10048..>10331)
                     /locus_tag="PLS35_13740"
     CDS             complement(10048..>10331)
                     /locus_tag="PLS35_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19779.1"
                     /translation="RYVISQYVQDQCGYVNMCWSWKRSLGFPISRLWEEMRHQWSTRN
                     AYLRIRGEIEIPRIVFLKTWEDLLSGHPIWFSGTILQLVLGKGLVYQEV"
BASE COUNT         2311 a         3121 c         2756 g         2143 t
ORIGIN      
        1 aaaggttggt ggtcccaacg ggatttgaac ccgtgtctga gccttgagag ggctccgtcc
       61 taggccaagc tagacgatgg gaccggagag gtagtccaac tgcagcggaa agaaccggac
      121 tgtaccatag cgagttttgg cttttcaaac ggcaagtcag aaggagcgga caccggggcc
      181 acacgaggca agtatccatg gcatgaacca gacctgcttg cagaggcttt tgaattagcc
      241 tagagtacga gtatgcatgg tcgttacttc ggatctcgag gttgtcttgt cgcaggcctg
      301 cttccagccc tgctgacgct ggctggctgc ggtgccagtt tcctggaagg cacgatcgcg
      361 ccgcccaact ccagcgcctg gacgaagtgc ggcagcacga ccaaagtgcg cgtgaagcca
      421 cctaaatcca ctattaccgt cgcctacacc gagcccacca ccggcacgga cggcaaacct
      481 ctgaccaatc tggcttatac caccatttac tataacgccg gggatggccc cgtgatcgcc
      541 aaggttgtgc cggcatcaca aaagaccggc ggggcagccg tttctcaagt gatcctgata
      601 cccacgccga gccaggcgga acaggaagtg accgtctgtg tgacggccac cgattccgac
      661 gaccgtgaag gccccgcaac cccctaggca ccgccagaac agacttgaca gcgccccgtt
      721 aagggcctac cttgcatccg acttgctcag gccgccacac agggccgaat gcgtcgccaa
      781 ccgggtcctc aggtcgcact agatttctac gcatggaact acgcaaacct gacgcgtcac
      841 atccttccca cgaggccgaa tcagaacggc acaaacacag cttggagatt cggctcggct
      901 ccaatatctt ccgcaatacc aacggcgtga tccgggttca gggcaaggaa caactcgtct
      961 tggaactggc accggaacaa gatcgcatcc tgctgacgat cgatctgtat gacgggagcg
     1021 gcaatcatgt ggcgcatctc cgccgcaatc ggtgggcgtt caacgacggc aatcgctttt
     1081 cgctcagcac aagcgattcc ccgccgaccc tgtttcccaa tcttccctgg ctcaagctga
     1141 cggatcagga aaccggcgaa acggttctgg aggcagcggt ggcgcccggc gaaaagatcc
     1201 acgtggcgac gggcaaattt tattctcacc gcggtcaact catcgaaatc acttcgcact
     1261 tctgccgcgt cggctccacg cataccctgt tcggagatgt gttcgaagcc cgcggcggga
     1321 cagcggtgct cggctgacgc cggccgaatc gtatgactga cagtccgaac cctagcatcc
     1381 aggccttcct ggtctgtgac agtgtcatcg aagacagcct caccaagaaa aaatctctca
     1441 tcggcatctt cacgcatctg caggccgcga cttttccatt tcagcaccat caattgggcc
     1501 tgtacttctg cctgactgat gcggaaggaa cgtaccacct ggagatcgat ctggtgtatg
     1561 tgaacaccga tcagttggtc tgccgcgcct cacttcccga catcgccatt cacgatcgcc
     1621 tgcagattgc ggatttcggc atcaacattc ccgctctgct gtttcccgct ccgggccgtt
     1681 atgagtttcg cctgcgcatg aacagtcgtg tcattgcgca aaaagacttc cacgtcatcc
     1741 aattgccggc gccaccggct gcctaatctg gccggctcac tagcgcacga aggcatacgc
     1801 gatcaggatg tcaggcatcc gaacccgaat ccgctagagg cagctcgcgc tcacctggac
     1861 ggggtgttga ggagggccag ccgcaaagga caggcgatcg gctagtagct agaaggccgt
     1921 ggcagtggca aggcaggcgt cgacatattc gttttcgccg cgatggcagg cctgtacgcg
     1981 cttatttttg tcgtcaaaca ggggcacgac catttctccc acggtggcat aacagtcccg
     2041 tttgaacggt ccatccaggc tgcggcacag cgcgaggccg ggctcgggat tggcgtcggt
     2101 cagaatgaag tccttcaccg caccgatgaa acattgcttg acctgatcgc cctggccaag
     2161 ccggcacaga gcattgacct gctgggcatc acgcaacgtg tagccgctga tatcccgccc
     2221 cagacttcga taacaggtcg tctgatgctc cccctccact cgagcgcatt gcgtgaacgc
     2281 ctgggcgaag tcgtaattca ggaaggtcag cacggcagag gtctgcatca ggtaacaggc
     2341 gcgcagatat ttttccgtca ggacggagca gggatacaac aaatcctgcg gattcaaaaa
     2401 actggtcgct tcatggtgat gatgctggct ggccgtttgc gcgcgacggg cctgctgaaa
     2461 cgtgacgatg ttctccatga acacgccgcc ataacaggat tcgcggtccc agctgcccgg
     2521 caaggcatca caaaacgcga ggctcttcag cacatcgtag cgaaattgaa tggtcaggcc
     2581 gtggcccaag ccatgaaggc agttgtaccg ctgaaacgaa taggccgaaa ccgtttcgct
     2641 gatggggcag agcggaagga tatgctccgg ttccacggcg ggcagactgc tcaggaaggc
     2701 ctgcaggacg ccgtgatagc aaccagacca gaagacatcc cggcagtgcg acatggcgag
     2761 cggggccgtt ccatagcgcg tgaatgactg ctggccgatg tggtggacga gcggatgggc
     2821 ctcgcgcagc gcttccggat cctgtgtcgt gatctgctcg agagccgtca gcgctccttc
     2881 ggtcccctcg gccagtaaca ccttttccaa gtgctgctgg tagcattcca tcttcgcctc
     2941 gtacggctgc gcacggcaga gctcggggga tgccggctgt tccgcgtggg cccaatccat
     3001 cccgcccagg ccacacagcg ccagaagtac cagaagcatt cgttgaccac gcatcatcca
     3061 atccccctta ctgaatcact acacgtcggg acatctgcca taaaaaagtg attctaaaat
     3121 actcctcccc atcgatccag agaaaatcaa cacggcacag gccgttcatc aatccccctt
     3181 ctcccgcctc cagttctatg atagaaccat tcgcgttcat caccggcaac tcgacggaat
     3241 cgcgcccagt atgtccacca cgccagaacc tccgacacgc tcagacttca ttcgcgagat
     3301 tgtggcagcc gatcgtgctg ccgggaaaca tgacggccga gtggtgaccc gctttccgcc
     3361 ggaaccgaac ggctatctgc atatcggcca tgccaagtcg atttgcctca acttcggcat
     3421 tgccaacgag caaccgggtg ggatctgcca tctccgcatg gatgatacga accccacgac
     3481 agaagacccg gaatacgtcc aggcgattca agaagatgtc cgctggctgg ggttcgactg
     3541 gcgcgacaag atgtttttcg cctccgacta tttcgagcgg ctctatggct ttgccgtcgt
     3601 gctcatcaag aaaggcctcg cctacgtcga tagcctgacg gcggacgaga tgcgccgtta
     3661 tcgcggaacg ctgacggaac cgggacagtc cagcccgcat cgtacccgca gcatcgagga
     3721 aaacctggac ctgttcagcc gcatgcgcgc aggggaattt cccgacggcg cccatgtgct
     3781 tcgtgccaag atcgacatgg cctcgccgaa catcaatctc cgcgatccgg tgctctaccg
     3841 catccgccat gtggcgcact accgcaccgg cacacagtgg tgcatttacc cagcgtatga
     3901 tttcgcccat ccgctgtccg atgcgatcga aggcattacc cattcgatct gcacgttgga
     3961 attcgaagat catcgtcccc tgtacgactg ggtggtggcg caatgcgaaa cacctcagcg
     4021 gccgcagcaa attgaatttg cgcggctgaa tgtgaccttc acggtcatga gcaagcgcaa
     4081 actcctcgat ctcgtcgaac gcaaactggt caacggctgg gatgacccgc gcctgccgac
     4141 actcaaagga ctccgccgac gcgggtttac gcctgaagcg ctccgtgcgt tttgcgaagc
     4201 cattggcgtg gcgaagcggg atgccatcgt cgaaatgcag ctgcttgaac actttgtccg
     4261 cgaagacttg aacaagcgcg ccccccgggt catggccgtg ctccgtccct tgcgcctcgt
     4321 gatcgaaaac tatcccgagg gacacgttga gcagctcgat gccatcaaca atccggaaga
     4381 tgcgtcggct ggaacccggc agttgccctt ttcgcgcgtg ctctacatcg accaggaaga
     4441 tttcaaagag gatccaccca agcaattctt ccgcctcgca cctgggcgcg aagtccgatt
     4501 gcggtatgcc tacatcatca cctgcgtcgg cgtcgtgaag gatccgacca ccggcgacat
     4561 cacggaactt cgatgcacct acgatcccga aaccaagagc ggcggagcgc aggcacaacg
     4621 aaaggtgaag gcgacgatcc actgggtgtc ggccggccat gccgtcgacg cggaaatacg
     4681 gctctatgaa agtctcatgc tgacggatcc ggccaaaatt cctgccgatc aggattggac
     4741 gcagcatctg aatccccgtt cgctggaacg gctgccatcg tgcaaagtgg aacccagtct
     4801 ggcctcaatc gctcccggca ccagcgtcca gtttgagcgc gtcggttact tttgtgccga
     4861 ccccgattct gcgccggaca aactggtgtt caatcgaact gtcacgctca aagacgcctg
     4921 ggcgaaaatc gagaaatccc acaccgcccg ttgaccagtt ccttcctcct tcagtcagcg
     4981 tcattccccc gcaaagtact ggcgcgccaa ccttctgcct ccttgaaccc tcacctgtcg
     5041 atgaacgaga atcacgcctc acgcagacgg tgaattcata cctagcaacg gggtggttac
     5101 gatggtgaca ggatccggca gcgacgatca gcaagaagaa cgcggttcat tccgggagca
     5161 gcttcgacga ttggaaactg cgataccggg cgccgcgagt agcgtgcaac cactccttat
     5221 gaacattcat cgctccgtcc gccgggtcca cgaccaactt cagcacatcg atgtgacgcc
     5281 tctcctgcct cggacgctcg tcagccacgt gcgctgcttt gattgcggca ccgccaagat
     5341 ggaatcgttt catacctcca cgcagggcgg ctaccatctt tgccctgcct gctttcagca
     5401 tcggctccac acgggacgcg ccaagaccgc tcattaacca ttccactcgc cgttgatctc
     5461 gacgtcgcgt taacacagga agcggcactc ctgccgctag ccctaggccc ttcccccacc
     5521 gtatactgca cgctttcact gttgcgagag aggtaatcag ggtatggccc ccaacattct
     5581 attgagctgc gaatcgatca gtaaaagtta tggggtacga gccctgttta gcaatctcac
     5641 gctggccttg tgcgaaggtg accatgtcgg cctgatcggc ccgaacgggt ccggcaaatc
     5701 cacgctgctt aaaatcctgg ccggtctcga atcgcccgac caaggcacac ggaccgtacg
     5761 gggtcatacc cgcatgggat atgtcccgca ggagccaagc tttcctgccg gctcgagcat
     5821 cgaacatgtc ctccagcaaa ccctcacgga tgccggtcgt gaccctcacg aagaaggcgg
     5881 cctcatcgcc cgagccctca gcatcggcac ctttcctgat ccggaacact gcgtcgacac
     5941 cctttcggga gggtggcgca agcgcctggc catcacacag gccttgctgc tcgagccgga
     6001 cgtgctgctc atggacgagc cgacgaacca tttggatgtc gagggcattc tttggctgga
     6061 gcgcctgctg aagaaccgcg ccaaggcctt tctcgtcatc agtcacgacc gtcgtttttt
     6121 agaatcgatt gcgacgcgca tggtggaact gaaccgctgt taccccgaag gccgctttga
     6181 agccaaaggc cgatacagcg actttttaga acaacgcgat gccgcactcc aggtgcaggc
     6241 ggactatcaa tcgtcgctgg ccaaccgtgt gcggcgcgaa gtggaatggc tgcgcagagg
     6301 gcccaaagcc cgcaccacca aagcgaaggg tcgcattcag tcggccggca aactcatcga
     6361 ggaattagac caggtcgaag cccgcgccgc acaatcgtcg atcggcatcg acttttccgc
     6421 ctccgggcga aagtccaaac aactgctggt tgcgaaagag gtcacgaagc gcttcaacga
     6481 gaagcccgtc gtcacgaatc tggatttgct tctcgggccg gggcaacgcc tgggcctcct
     6541 tggcccgaac ggcagtggga agaccactct ccttcgatta ctcgccggca cgctggaacc
     6601 cgactcaggc acaattatgc gcgccgatgg actgcgcatc gtttccttcg aacaacatcg
     6661 tgaatccctg gatcaagatg ctccgctgcg gcgcgcgctg gctccttcag gcggcgatgc
     6721 gatcgtctac cagggccgct cagtccatct tgcgtcctgg gcgaagcgct ttctctttcg
     6781 acctgaacag ctcgaccttc cagtctcccg gctttcgggc ggtgaacagg cgcgactgct
     6841 gatcgcccaa ctgatgttgc aaccggccga cctgctcatc ctcgacgaac cgaccaacga
     6901 tttggatatt ccgacactcg atgtgctgga agacagctta ctcgaattcc ccggcgcact
     6961 ggtgctcgtc acccatgatc gctggttgct ggatcgtgtc tctaccattc ttctggcact
     7021 ggacggcacc ggtcgcctcg aatggttcgc cgactatgcc cagtgggaaa gcgcgcagga
     7081 acgggctgcc gagtccgaaa cgggcccgaa tgaggaaagc accacgaccg gcgatgtggc
     7141 aggaacagca cccattcgca agcccaagaa actcagttat cgggaacaga aagagtgggg
     7201 cacgattgaa gacacgatcc tgaaggccga ggagcaggtg gccgcctgtc aggccgccat
     7261 gcacgatccg aaggtcatgt cgaacgccgc ggaactacag gcacggagcg aggccctggt
     7321 ggcggcgcaa gctgaagtgg aacggctcta cgcccgctgg gccgagttgg atgaaaaacg
     7381 cgcagagacc gtgcaaagtt cgtgaaggga atatttggct gggggactag gaatcgaacc
     7441 taggtagcca gctccaaagt cgcaaaaagc accctagaga cggtgaagga tcttagagga
     7501 aggattgcca tttttccctt atgaaacaaa atcttcacca acgatcgatg agattagtta
     7561 cgattagtgt acacctgtaa tcgtgcccta aaccgtgact gaaccgtgac caaaaccgtg
     7621 accggggact tatcaaaaat tggacacaat tttgaagatg aatcatgaaa tggctcacgg
     7681 actatatgca ggtcgaagta gaattttcac tatcgattga ccatcctctc tccttcagag
     7741 tatcatcagt ttccacacta aaggtgtaat catgatcaat gataacgacg ctttaccgct
     7801 tgaatacaag ctgaaggaag ggcgcgccag cgggcagctt gagctcacgg acatcgtgaa
     7861 taatcccctt agtgcgggcg tagtccgggt ctttggtgac agcctccaaa aacaactgtt
     7921 tcgcttcgtc gggagtgagt ccggttcgct cacagagaat ctttgcaata cgagcctgat
     7981 ccgcctggat tgagtccatt ctctcgcgca gggtcttttc ttcgaatcgt gctgcgccgt
     8041 tgatatcaaa ccccacacca tgaaacatga aagtcgcatg agggcttgaa taccgttgtt
     8101 ctccagcgag gaacaagacg ttacctatag agtcgacatt tccgacatta tgagtaataa
     8161 gcttgaaggg catggcacga agcacattgt aaagatttag cccattcata actgtgccac
     8221 ccggcgttga aagcataaga tagaccgtgg aaaccttttt ctcgactaac tgaccacata
     8281 gacccagtaa accttccgtg gtcgcctgat ttacttccgc gctaaaagag atgtaaacgg
     8341 tgtcattcga cataaaaccc tccttggtaa gttgatatga ttgttgacaa accaggaggg
     8401 atcaggcagg ataacaacag cctacagctc tctccggttc gatttggtgc ccctggccgg
     8461 aattgaaccg acgacaggcc cgttagcggc ggggtgctct gtccagctga gctacagggg
     8521 cggacgttag agtaaaccgc ctgatggtag cagccatcag gcggtttctt tttgaatctg
     8581 tacctcctca tctgtcggaa gacgatactt tcctcttcct tctcgcacta ttcggttctc
     8641 cttcgccaaa cgagacagga ctccagtcat tacattctgc gggacaactc gtcccgtttt
     8701 tgccagctct ccccttatct ccgccggccc caacggagcc ggtgaattac aaagcgtagc
     8761 cttaacctca tccactaatg tcatctcacg tttcagcgcc ttgtactccc cgacggtgta
     8821 ctgcttattg tacctttcgc accaataatc ccaggtgccg tcatgtattg tcttgggacc
     8881 gtgaaacata ctttgaggag gggagacggg gccattcagc ggaagctgcg gggaatccgg
     8941 ttcccactga gccagaagag cttgcttggt gacaatctgc ctggcggtcg taagtatctc
     9001 caattcgtgt tgagcctgac gcagtaattc gacagcttga tcgacttcct tttcaagccg
     9061 gcgcaccttc tctttacgtt cttctattgc ctgttcaaga tcacccatac cgcttacgtt
     9121 acacgacact gtgctacttt gcaaatattt ttcacagact acagtagcct tgtagctaat
     9181 cttagcataa ctaattgata atgctagtta ttttataatt attcaatgct acaaatttta
     9241 atgtttcacc gctccattgt caaatctggg cggcctacga gatcatatcc ctccgaagga
     9301 agccgatgtc gccagcccgc tcacgagatt ttttcggtgt gcggcatcag ctcggcgacc
     9361 tgcaacaaat agaaagttaa tgctgcagtc ttggtgtgac ctcggctcgc tccccttccg
     9421 gtctgagact tcgccgagag ggagaaggca tgccccatga gccttaactt ccgttaccca
     9481 actccttcag ccgacacccg ttgacgttgc gtcctacttt gaccatatag atcaagtgaa
     9541 gcgggggctg cgcccccgat ccccccggcc gtccggtcca gtggtccgga gcggccgggc
     9601 ggcctcgttg ggccgagtct ttgcgttcgt aatctcccca tggtttcagt attcctcttc
     9661 tctactctgt ctgggtaagc aacgatccaa cagaatggcg gccaagctga cgcatcgccg
     9721 ccctgcatat ggacggagca tcgttccctc tcacccgtga gggatagtgg acgggtgaca
     9781 gggagaaatg gattagagtg tttcaccgtc gctccgtcat tcccgcacag tcgatacaaa
     9841 gccagatttc gcacgtcatg tcgttgtctc gataacagaa gcgtacccac gctcgttcga
     9901 attgacagaa ctcacacagc cgttcctttg tatgttcagg acagagccaa gcctttctcg
     9961 cccgatcgta cgtggtcgct tcccgtccgc aacaggacgg accgaagaga cttggcagct
    10021 tcagatcgca tcgatgccac atcattctca cacctcctga tagaccagcc ccttgcccaa
    10081 cacgagctgc agaatcgtgc cgctgaacca aatcggatgg cctgagagca gatcctccca
    10141 ggtcttcaga aagacgattc gagggatttc gatctcgccc ctaatccgga gataggcgtt
    10201 ccgagtggac cattggtgcc gcatctcttc ccacagtcgg ctgatgggaa agccgagcga
    10261 gcgtttccac gaccaacaca tattcacgta gccgcattga tcctggacgt attggctgat
    10321 gacgtagcgg c
//
LOCUS       DAOQEW010000136        10322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2021_length_10322_cov_7.944288, whole genome shotgun sequence.
ACCESSION   DAOQEW010000136 DAOQEW010000000
VERSION     DAOQEW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_10322_cov_7.944288"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PLS35_13745"
     CDS             <1..1350
                     /locus_tag="PLS35_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HNI19780.1"
                     /translation="DVTTRQQVQLRHLTIAHVPAVFAKLEEVGLTSMQTGMDSVRNVM
                     TCPVAGLNPNELLDGTEIVRAINHEVLGNPAYTNLPRKCNIAVTGCTDNCLHTETQDI
                     ALVPAYHDLGHDKCFGYNVLVGGKLGSGGYRIASSLDMFVNPAEAFDVCRTLLHIYRD
                     HGPRENRTQARLAFLVEAWGESRLRHEVEVRMGRALPSAGVDARAAGERDHIGIFRQK
                     QRGMNYIGLKILVGRIKAGDLRNIAALAERYGTGEVRLSPAQAIVIPHISDRVVGELA
                     EEPLVKQFAYNPSPLYKGLVSCVGSDYCNMAVIETKRRAVETAHILERKLEGLKPITL
                     HWSGCPAGCGNHLVADIGLLGKKARVGGKVVDAVDIFVGGRSGPDPKLATKIMEDVPC
                     DKLPAVLENLMPYHTRDKMHRVRGKAVAKSTASAKSASSANAADGAISGLRPQPFPA"
     gene            1413..2189
                     /locus_tag="PLS35_13750"
     CDS             1413..2189
                     /locus_tag="PLS35_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI19781.1"
                     /translation="MAKRTKSSQEPADLLSRHIEEIRETLAAFQPFLSRRKATASLEG
                     FDEQAEEVLSETFGGASDELEAYYYAKLGEAAILPEEAQEAGTHNLERESLHQRKQVL
                     ESCLAQLELKKSTRVGRDWRRTLGERIDGRTVAEFMSSEVRSVHKGASIKEAGRLLQK
                     WRIGSLLVDDGSRYLGIITDTDLSRKAVAKGLDPNSTTVMSCMSKAVVTIEDSEPIKE
                     ALRLMKKEGIRHLPVTEDGTIIGVLSVGDLLRAYQKMLEL"
     gene            2507..3676
                     /locus_tag="PLS35_13755"
     CDS             2507..3676
                     /locus_tag="PLS35_13755"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNI19782.1"
                     /translation="MHELANGRSVRKWPLSIPAGKTSLPLMFILSFLVLAGLPWAWGA
                     VDAAEVPTTPGIVKWHPGHYYAPMTFMRANANVMTQIYAELKATPALRGLQVRFAWPE
                     LEPEEGRYDFKGIEKVLAELAPLNKQLVILLELKSFNPQILPMPTYMRTEAYEGGAFP
                     FSSYGQSIPRGYNLKLWNSGVHDRLVALLTELGKRFNGHANFEGIGLPETALGQPIEL
                     LSSHDTEKFYDNLLDVQRQMRAAFPNTVTYQFVNYPREILPGFIEHMRTIGTGLGGPD
                     SFLDDPGLNFDHPNKPKGIYSYYPQMSGLIPLTPSVMQANYDNTRHDGKGRVPTVAEL
                     LAFGRDRLRANYLFWTRAPGHFQQVLEQMQRIPLQGNPSGGLDAVCPKAYVSCVE"
     gene            complement(3749..4177)
                     /locus_tag="PLS35_13760"
     CDS             complement(3749..4177)
                     /locus_tag="PLS35_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19783.1"
                     /translation="MLTRVLAGLLLSVVLASCGQGDGGAGSGIKPVGEWAKIGETDTY
                     IYYADYASIKKADETVVMLDLFDYKSAQSEGGGAPALSKATEREYDCQNKKSQPLKSS
                     WYSEGMGAGTVVRSTGTSNQWSSAAQGTATGGLLKAACGN"
     gene            complement(4379..6655)
                     /locus_tag="PLS35_13765"
     CDS             complement(4379..6655)
                     /locus_tag="PLS35_13765"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19784.1"
                     /translation="MLHANTDRDQILATLAPLCPGIETDILQDFTSRMDADYFAAFSP
                     KLLAEHLSLAALLTPDHPCEVRFAKLDRKRLTITIVAYDYFSEFATICGLLSAFGLNI
                     EEGRIFTSAESEPARGGRQNSPYGTRPRPQGRPGLTRKKIVDVFTVTPTEGEPFTADD
                     QKRLAEQLSRMIILLDTNEFDEARQQVNRQLIEYLGTRRSSFSGLLHTVHITFDNSQS
                     ATDTIMDIRSDDTPAFLYAFANALAMRNVYISKAQFAIEEGKLHDRFYVRNRFGQKLL
                     DPGDQEQLRLTAVLIKQFTHALTWAPDPAKALESFDQFLDLVLEGSRQAGKKQAWDFI
                     NDKKTFPLLARLLGASDFLWEDFLRRQHVNLLPLLKDYRDAPLIKPQATLRKELNRLI
                     AKAKTDEARKEALNRFKDQELFRIDMKHIVEPGTNLPDFSLAISELAEVIVERSLLDC
                     QAKLTKRYGSPRLANKKPCPFAILGAGKFGGRELGYASDIEVLFVYGGPGHTTGKDGI
                     ENSEYFERLAQELLQWIEAKQEGIFHIDVRLRPHGGKGSLSNAFDEVCKYYSADGLAA
                     PFERQALIKLRHIAGDAALGRKVEAHRDSFVYSGAPWDLTTAIDLRRQQVKQLVEPGQ
                     VNLKHSHGGIVTLEYAIQYLQVMHGHRHASLRTPNTLRALAALIDADLIPRTTGENLR
                     KSYLFIRMLIDGLRMVRGNTKDLVLPPPDSDEFIFLARRVGYQTEDWQAGAKHLLSDI
                     EQHMKQNREFFEKMFGKV"
     gene            6726..8540
                     /locus_tag="PLS35_13770"
     CDS             6726..8540
                     /locus_tag="PLS35_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19785.1"
                     /translation="MARRSSQNDSLADPAGTGASPVFPDPTPLLVASGLDADEVVRIL
                     GPYGLGNLTEADTNIQSMAGEPHTRRLLAGILPRLLAEVARTADPDQALNHWERLFAG
                     SANRSSLLQYLQASPRMLGLLCTIFGNSDSLAFALIRDPMLVYWLAEEDVLTTAPTRE
                     GMVAALRQSLGSLKATELKLDVLRRVRRREMLRIGVRDLLRLATVEETVGSLSDLASV
                     LIHAAYEIVDKDLKTQHGTPMHQRPDGTWIETEFVVIGMGKLGAHELNYSSDVDLIYV
                     YASADGETRKAKGNRQASIKPLSNEEYFEMLARSLTKALAEQTREGAVFRVDLRLRAE
                     GTVGQLARSVDAYAKYYAQRGQVWERLALLKAWPVAGSPAVGQAFVQMAEGFVFQSDL
                     QPLTRERALAVIRDVRSVKEMIDEKIAVRGHQHRNVKLGTGGIREIEFLVQTIQVLIG
                     GAVPDILDRKTVGALHRFCRHRFVNEDDQQQLTEAYWFLRDVEHKLQMMHDLQTHALP
                     DSHEELQRCAIRMGYYGESRKRAMEQFLADRQRHTAVVHQTFRSLFYHPETSDLLSAV
                     VAAAEGHSRRNPSRLEKETGPAGPPPDRSSTAKKKRKP"
     gene            complement(8537..10180)
                     /gene="groL"
                     /locus_tag="PLS35_13775"
     CDS             complement(8537..10180)
                     /gene="groL"
                     /locus_tag="PLS35_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247953.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNI19786.1"
                     /translation="MMAKQLLYSEAARAAILRGVNQLADAVKATLGPKGRNAILDKKF
                     GAPTITKDGVTVAKEVELKNPYENMGAQLVREVASKTSDTAGDGTTTATVLAQAIYRE
                     GVKNITAGANPMEIQRGINKAVEVVIGELKKLSKPCQNKTEISQVGTISANNDKTIGD
                     LIAEAMEKVGKDGVITVEEAKSMTTSLDVVEGMQFDRGYISPYFVTNAERMEAVMDEP
                     LILINEKKVSSMKDLLPVLEQVAKLGKPLIIIAEEVEGEALATLVVNKLRGTLNVSAV
                     KAPGFGDRRKAMLEDIAILTGGQVISEDLGLKLENVKLTDLGRAKRVTIDKDNTTIVE
                     GHGDPKKIEGRVKQIKAQIEETTSDYDREKLQERLAKIVGGVAVINVGAATETEMKEK
                     KARVEDALHATKAAVEEGIVPGGGTAYLRCLKGLDSLKDLPLEQKVGVDIVRRSLEEP
                     VRQIAANAGAEGSVVVGRVREDKNPNGGYNAAADEYVDMIKTGIIDPTKVSRCALQNA
                     ASVAGLMLTTEVMITELPEEKKEPAGGHAGHNHGMEGMY"
     gene            complement(10227..10322)
                     /locus_tag="PLS35_13780"
     CDS             complement(10227..10322)
                     /locus_tag="PLS35_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19787.1"
                     /translation="MFDKYSGSKLRIEDEECLILKEEDILGIFTH"
BASE COUNT         2019 a         2862 c         3182 g         2259 t
ORIGIN      
        1 gatgtgacca cgcggcaaca agtgcaactc cgtcacctga cgatcgcgca tgtgccggcg
       61 gtgttcgcga agctggagga ggtcgggctt acgtcgatgc aaaccggcat ggacagtgtg
      121 cgcaacgtga tgacctgtcc ggtggcgggg ttgaatccga atgaattact ggatgggacg
      181 gagatcgtcc gcgccatcaa ccatgaagtg ttgggcaatc cggcttatac gaatttgccg
      241 cgcaagtgca acatcgccgt gaccggctgc acggacaatt gtctgcatac ggagacgcaa
      301 gatattgcgc tggtgcctgc gtatcatgat ttagggcacg acaaatgttt cggctacaac
      361 gtgttggtcg ggggcaaatt gggatccggc ggatatcgca tcgcgagttc gttggatatg
      421 ttcgtgaatc cggccgaagc attcgatgtc tgccggacgt tgctgcacat ctaccgtgat
      481 catggcccgc gtgaaaatcg cacccaagcg cgcctcgcgt ttctggtcga agcctggggc
      541 gagtcccggc ttcggcacga ggtggaagtg cgcatggggc gcgcgttgcc gtctgcaggg
      601 gtcgatgcca gggcggcggg tgagcgcgac cacatcggca ttttccgtca aaaacaacgc
      661 ggcatgaact atatcggtct caagatattg gtgggccgga tcaaagcggg tgatttgcgc
      721 aacatcgcag cgctggcaga gcggtatgga acgggtgagg tgcggctgtc gcctgcgcaa
      781 gctatcgtga tcccgcacat cagcgaccgt gtggtgggtg aactggcgga ggagccgctg
      841 gtaaagcaat tcgcctataa cccatctccc ctgtacaagg ggctggtcag ctgcgtgggg
      901 agcgactact gcaatatggc cgtcatcgaa accaagcggc gggccgtgga aaccgcgcac
      961 atcctggagc gcaagctgga agggctgaag ccgatcacgc tgcattggtc cggctgccct
     1021 gccgggtgtg gcaatcacct ggtcgcggat atcggactgc ttggcaaaaa agcccgagtg
     1081 ggcggcaagg tggtggacgc cgtcgatatc tttgtcggcg ggcgttcagg tccggacccg
     1141 aagctggcga cgaagatcat ggaagatgtg ccgtgcgaca aactcccggc ggtcctcgaa
     1201 aacctgatgc catatcatac acgcgataag atgcaccgtg tgcggggcaa ggccgtggca
     1261 aaatcgactg cctcggcgaa gtcagcctct tcggccaatg ctgcggatgg ggcgatctcc
     1321 ggcctgaggc cgcaaccgtt tccggcctag cccgtgagac ttgagccgat atgggagttg
     1381 ggttattctc ttgccttcac gaaggaggta gcatggccaa gagaacgaaa tcatcgcagg
     1441 aaccggctga tctgctcagc cggcatatag aagaaatacg tgagacattg gccgcgttcc
     1501 agccattttt atcgcggcgg aaggccacgg catcactaga aggttttgac gagcaggccg
     1561 aagaggtgct cagtgaaacc ttcggcggcg cgtccgatga gctcgaagcg tattactatg
     1621 ccaagctcgg tgaagcggca attcttccgg aggaagcgca agaggccggg acgcataatc
     1681 tcgagcgtga gagcctgcat caacgtaagc aggtcctcga gagctgcctc gcgcagcttg
     1741 agctcaaaaa aagcaccagg gtcggccgag attggagacg gacgctcggt gagcggatcg
     1801 atggccgcac cgtggccgag ttcatgtcgt ctgaggtcag gagcgtgcac aaaggcgctt
     1861 cgatcaagga ggcaggccgg ttgctgcaga agtggcgtat cgggtcactg ctcgttgatg
     1921 acggatcacg ctatctcggc atcatcacgg acacggatct gagccggaag gccgtcgcca
     1981 aggggttgga tccgaacagc accaccgtca tgtcatgcat gagcaaggct gtggtgacca
     2041 ttgaagatag tgaaccgatc aaagaagcgt tgcgactgat gaaaaaagaa ggcatccggc
     2101 atctgccggt gacagaagac ggcaccatca tcggggtgtt gtctgttggc gaccttcttc
     2161 gggcctatca aaaaatgctg gagttgtaag gagaaggttt tctatgccaa accggcggcg
     2221 cacgagcgct gccggtttgc gccgtgctcg aacagtttcg ccatgaccgc cgtttggcgc
     2281 ccttcctgag atgtcacctt tctctgactc acgaaccacc taattcatgt cgagtgccgg
     2341 ctcgctcgac aatggaggag agggggccga ttttcctcat tgcctccact acataagtac
     2401 tgaaccccac aggacagtac ggtgggagcc tctcgtgcta tcatgcgcct gcagtgagag
     2461 gcgcggcggt ctttcactgg cagtagacct atcggaaagg agacgtatgc acgagctagc
     2521 gaatggacgg tcagtacgta aatggcccct ctctatccct gccggaaaaa catctctccc
     2581 tctgatgttt atcctgagct tcctcgttct cgcgggcctg ccatgggcgt ggggcgcagt
     2641 ggatgccgcc gaggtgccaa ccacccccgg catcgtgaag tggcatccgg ggcattatta
     2701 tgcgccgatg acctttatgc gggcgaatgc gaatgtcatg acgcagatct acgccgagct
     2761 gaaggcgacg cctgcgctac gcggtctcca agtgcggttt gcctggccgg aactagaacc
     2821 ggaagaaggc cgctacgact tcaaagggat cgaaaaggtc ctggccgaat tggcgccctt
     2881 gaacaaacag ttggtcattc ttctcgaact gaagtctttc aatccgcaaa tcctgccgat
     2941 gccgacctat atgcgaacgg aagcctacga agggggcgcc tttcccttca gcagctatgg
     3001 gcagagcatt cctcgcgggt acaacctcaa actctggaac tcgggtgtgc acgatcgact
     3061 ggtggcgctg ctgaccgaac tgggcaagcg tttcaacggc catgcgaact tcgaaggcat
     3121 cggtctcccg gagactgcct tggggcagcc gatcgagctt ctgtccagcc acgacaccga
     3181 aaagttttac gacaacctgc tggatgtgca gcggcagatg cgtgccgctt ttcccaatac
     3241 cgtgacctac cagtttgtga actatccgcg tgagatattg cccggcttca tcgagcacat
     3301 gcgcacgatc ggtaccgggt tgggcggccc ggatagtttt ctagatgatc cgggattgaa
     3361 cttcgatcat ccgaacaagc ccaaggggat ctattcctat tacccgcaga tgtctggcct
     3421 gattcccctg acgccctcgg tcatgcaggc gaactacgat aacaccaggc atgacggaaa
     3481 ggggcgagta ccaaccgttg ccgaactgct ggcgttcggc cgggatcgat tgcgggccaa
     3541 ctacctgttc tggacgcgcg caccggggca tttccagcag gtgctggaac agatgcagcg
     3601 tattcccttg caggggaatc cgtccggggg gcttgatgcg gtctgtccca aggcgtacgt
     3661 ctcctgcgta gaatagcggc agccggatgg caggtattgc ctgccggctc gagtaacgta
     3721 gcgcgaggcg gcgtgtttcg gccgtcggct aatttccgca ggcggccttc aacagccctc
     3781 cggttgcagt cccttgagca gcagaggacc attgattcga ggtgccggtg gagcgcacga
     3841 cggttccggc tcccatccct tcggaatacc agctggactt gagcggctgg ctcttcttat
     3901 tttgacagtc gtattcgcgc tcggtggcct ttgacagggc tggggctcct cccccttcgc
     3961 tctgtgcgct tttgtagtcg aacaggtcca gcatgacgac tgtttcatct gcttttttga
     4021 tgctggcgta atcggcgtaa tagatgtagg tatccgtctc gccgatcttg gcccattctc
     4081 caaccggctt gatgccggag ccggctccgc cgtcgccttg cccgcatgaa gccagcacca
     4141 cgctcaataa cagccctgcc aatactcgcg tcaacatggt aatcctcctc ccgaagtgac
     4201 tccgccgttc cactgtacca tggtttggcc aaaactcgac gcgggggtca tgcgctacgt
     4261 aggtaacgag aagcagagga cgagaggtgc ttgtggaacg gcccttccag cagatagggc
     4321 ggggctcaat gttacgaaga cgatggagcc ggcaatgact tcagtggcct gcgtggcctc
     4381 acacctttcc gaacatcttc tcgaagaact ctcgattctg tttcatgtgc tgttcgatgt
     4441 ccgacagcag gtgtttcgcc ccggcttgcc agtcttctgt ctgatatccg acgcggcggg
     4501 cgaggaagat gaattcgtcg gagtccggag gcggcagaac gagatccttg gtgttgccgc
     4561 gcaccatgcg caggccgtcg atcagcatgc ggatgaagag gtaggattta cgcaaattct
     4621 caccggttgt acgtggaatc aggtccgcgt cgatcaaggc ggccaacgct cgcagcgtgt
     4681 taggcgtacg aaggctcgca tggcgatgac catgcatgac ctggaggtat tggatcgcat
     4741 attccagcgt gacaatgcct ccgtggctgt gtttgagatt gacctggccc ggctcgacga
     4801 gttgtttgac ttgttggcga cgcagatcga tcgcagtcgt cagatcccag ggagcgccgc
     4861 tatacacaaa actgtcgcga tgggcttcca ccttcctgcc gagcgcggcg tctcctgcga
     4921 tatgacgcag cttgatgagc gcctgccgct cgaacggcgc cgctaagccg tcggcgctat
     4981 agtatttaca gacctcatca aacgcattgg agagagaccc cttgcctccg tgtggtcgga
     5041 ggcggacatc gatatggaag atgccttctt gcttggcttc tatccattgc aacagttcct
     5101 gtgccaagcg ctcgaaatac tcgctgtttt cgatgccgtc tttcccggtc gtgtgaccag
     5161 gcccgccata gacgaacaac acctcgatgt cggaggcata tccgagttcg cggccgccga
     5221 attttcctgc acccaggatg gcaaacgggc agggtttctt attggcgagt cgaggcgatc
     5281 catagcgttt agtgagcttt gcttggcagt cgagcaagct gcgctcgacg atgacttcgg
     5341 cgagttcgga aatggccaaa gagaagtcag gaaggttggt tcccggctcg acgatgtgtt
     5401 tcatgtcgat acggaacaat tcctgatcct tgaagcggtt gagcgcttct ttgcgtgctt
     5461 cgtcggtctt ggctttggca atgaggcggt tcagctcttt ccgcagcgtg gcttgaggct
     5521 tgatgagcgg cgcgtcgcga tagtccttga gcagcggcag caggttgaca tgttgccgcc
     5581 gaagaaagtc ttcccataaa aagtcactgg cgccgagaag ccgcgcgagc agtggaaacg
     5641 tctttttatc attgataaag tcccacgcct gtttttttcc ggcttggcgg gacccctcaa
     5701 gcacaaggtc gaggaactga tcaaacgatt ccagcgcttt agcgggatcg ggtgcccacg
     5761 tgagcgcgtg tgtgaattgt ttgatgagca ccgcagtgag ccggagttgc tcttgatcac
     5821 ccggatcaag caacttttgc ccgaagcgat ttcgaacata aaagcggtcg tgcaactttc
     5881 cctcttcgat cgcgaactgg gccttgctga tgtagacgtt acgcatagcc agcgcattgg
     5941 cgaaggcata gagaaacgcc ggcgtgtcgt cggaccggat gtccatgatc gtgtcggtgg
     6001 ccgactggct gttgtcgaag gtaatgtgaa cggtatggag caggccgctg aatgaactac
     6061 gccgtgtgcc caagtattcg atcagctgcc tgttgacctg ctggcgtgct tcatcgaatt
     6121 cgttggtgtc cagcagaatg atcatgcggg acaattgttc ggccaagcgc ttttgatcgt
     6181 cagcagtaaa gggctctccc tccgtaggcg tgaccgtgaa gacgtccaca atcttttttc
     6241 tcgtcagtcc cggcctgcct tgcggtctgg gacgtgtgcc gtagggactg ttctggcggc
     6301 cgcctcgcgc cggttcgctt tcggcagaag taaagatgcg gccttcctcg atattcaaac
     6361 cgaaggccga gaggaggccg cagatggtgg cgaactccga aaagtagtcg tacgcaacga
     6421 tggtgatggt caaccgtttg cgatcgagtt ttgcgaatcg cacttcgcag ggatggtccg
     6481 gcgtcagcaa tgcggccaac gagagatgct cggcgagcag tttgggagaa aatgctgcga
     6541 agtaatccgc gtccatcctg ctggtaaaat cttgcaggat gtcggtctcg atgcccgggc
     6601 agagcggcgc cagggtggcg aggatctggt cgcgatcggt gttcgcgtgc agcatggtgt
     6661 ccgtggagta tacccgaagg tgctgtcgtt gtaccggaag aattgcgcca gtataatgcg
     6721 gcgccatggc acgacgttcc tcccagaatg attcgctcgc tgaccctgca ggaacgggcg
     6781 cctcgccggt gttccccgat ccgaccccct tgctggtcgc atccggtctt gacgcggacg
     6841 aggtggtgcg gatcctgggg ccgtatggac tagggaatct caccgaggcg gatacgaata
     6901 ttcaaagcat ggcaggcgag ccccataccc gccgcctgtt ggctgggatt ctcccgcgtc
     6961 ttttagctga ggtcgctcgt accgccgatc cagaccaagc gctgaatcat tgggaacgtc
     7021 tttttgctgg aagtgcaaac cgttcctctc tcctgcaata tctgcaagcc tcgcccagaa
     7081 tgttggggct gctgtgtacc atcttcggaa acagtgattc tctggcgttt gcactcatcc
     7141 gcgatccgat gctggtctat tggctggcgg aagaagatgt attgacgacg gctccgaccc
     7201 gggaggggat ggtggccgcg ttgcgccaga gtctcggctc tctcaaggcc accgaattga
     7261 agctcgacgt gctgcgtcgc gtgcggcgac gggaaatgct acggatcggc gtacgcgatc
     7321 ttctgcgcct ggccactgtg gaggagacgg tcggatcgct gtctgatctg gcgtcagtat
     7381 tgatccatgc ggcctacgag atcgtcgaca aggatctcaa aacccagcac ggaaccccga
     7441 tgcatcaacg gccagacggc acctggatag aaaccgaatt tgtcgtgatc gggatgggta
     7501 agcttggcgc gcatgagttg aattacagct cggatgtcga cttgatttat gtctatgcat
     7561 ccgcagacgg tgaaacgcgc aaggcaaaag gcaatcgcca ggcgagcatc aaacctctgt
     7621 ccaatgaaga gtattttgaa atgctggcgc ggtcgctgac gaaagccctg gcggaacaga
     7681 ctcgtgaagg ggcagtgttt cgcgtggatc tccggcttcg cgccgaagga accgtggggc
     7741 aactggcgcg gtcggtggac gcctatgcga agtactatgc gcagcgcggc caggtctggg
     7801 aaagactggc actgctaaag gcttggcctg tcgccggttc gcctgcggtc gggcaggcgt
     7861 ttgtgcagat ggcggaaggg ttcgtgtttc aatctgatct ccagcccctt acgcgagaac
     7921 gggccttggc ggtgattcgc gacgtccgtt cagtgaagga aatgattgac gagaagatcg
     7981 cggtgcgcgg gcatcagcat cgcaatgtga agctggggac cggaggcatt cgggagatcg
     8041 aattcctggt acagacgatt caggtgctga ttggtggagc agtgcccgat atcctggatc
     8101 gtaagaccgt gggagctctg catcgtttct gccggcatcg cttcgtgaac gaagacgatc
     8161 agcagcagct gactgaggcc tattggtttt tacgggatgt cgaacacaag ttgcagatga
     8221 tgcacgatct gcagacgcat gcgttgcctg acagccatga agagctgcag cgttgtgcga
     8281 ttcgaatggg ctactacggg gagtcgcgca agagggcgat ggaacagttc ctcgcggatc
     8341 ggcagcggca tacggcggtg gtgcatcaga cgttcagatc gctcttctac cacccggaaa
     8401 cgtctgatct cttgtccgcg gtggtggctg cggcggaagg ccactcccgc cgcaacccgt
     8461 ctcggctcga aaaagagacc ggcccggcgg ggccaccgcc ggaccggtct tcgacagcga
     8521 agaagaaacg gaaaccttag tacatccctt ccatgccgtg gttgtgacca gcatggccac
     8581 cggcaggctc tttcttctct tccggcaatt ccgtgatcat gacttcggtc gtcagcatca
     8641 aaccggcgac gctggcggcg ttctgcaatg cgcagcgcga caccttggtc ggatcgatga
     8701 tgccggtctt gatcatgtcc acgtactcgt cggcggcggc gttgtagccg ccgttcgggt
     8761 tcttgtcctc acggacgcgg ccgaccacca cggagccttc ggcacccgcg ttggctgcga
     8821 tctgacgaac cggctcctcg agtgagcggc gcacgatgtc gacgccgact ttctgctcca
     8881 ggggcaagtc cttgagggaa tccaagccct tgaggcagcg cagataggcc gtgcctccgc
     8941 caggaacgat gccttcttcg acggccgcct tggtggcgtg caacgcgtct tcgacgcgcg
     9001 ccttcttttc cttcatttcg gtctcggtcg cggcgccgac gttgatgacg gccacaccgc
     9061 cgacgatctt cgccaaccgc tcctggagct tctcgcgatc gtaatccgag gtggtttcct
     9121 cgatctgggc cttgatctgc ttcacgcggc cttcgatctt cttcgggtcg ccatggcctt
     9181 cgacgatcgt ggtgttatcc ttatcgatcg tgacgcgctt ggcgcggccc agatccgtca
     9241 gcttgacgtt ctcgagcttc aagccgaggt cttcggagat gacctggccg ccggtgagga
     9301 tcgcgatgtc ttccagcatc gccttgcggc gatcgccgaa gcccggtgcc ttcacggccg
     9361 acacgttgag ggtgccgcgg agcttgttga ccacgagggt cgcgagcgct tcgccttcga
     9421 cttcttcagc aatgatgatg agcggcttgc cgagcttggc aacctgctcg aggaccggga
     9481 ggaggtcctt catgctgctg actttctttt cgttgatgag gatgaggggc tcgtccatga
     9541 cggcttccat ccgctcggcg ttggtcacga agtaggggga gatgtagccg cgatcgaact
     9601 gcataccctc gacgacgtcc agcgaggtgg tcatcgactt ggcttcttcc accgtgatga
     9661 cgccatcctt gccgaccttc tccatcgctt ccgcgatgag gtcgccgatg gtcttgtcgt
     9721 tgttggcgga gatggtgccg acctgggaga tctcggtctt gttctggcag ggcttgctga
     9781 gcttcttcaa ctcgccgatc acgacttcca cggccttgtt gatgccgcgc tggatttcca
     9841 tcggattggc acctgcggtg atgttcttga cgccttcccg gtagatcgcc tgggccaaca
     9901 ccgtggcggt ggtggttccg tcaccggccg tgtcgctggt cttgctggcg acttcgcgaa
     9961 ccaactgagc gcccatgttc tcgtacggat tcttgagttc gacttccttc gccaccgtca
    10021 cgccgtcctt ggtgatcgtc ggggcgccga acttcttgtc caaaatcgcg ttccggccct
    10081 tggggccgag cgtcgccttc acggcatcgg cgagctggtt cacccctctc aggatggctg
    10141 cccgtgccgc ttcgctatac aacaattgct ttgccataat cgtcctccgt taatcgtcag
    10201 aataatcgtt agtcgggtgc gtgagattag tgggtgaaga tacccaggat gtcttcttcc
    10261 ttgaggatga ggcactcttc gtcttcgatc cgcagcttgc tgccggaata cttgtcgaac
    10321 aa
//
LOCUS       DAOQEW010000137        10271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2041_length_10271_cov_8.002643, whole genome shotgun sequence.
ACCESSION   DAOQEW010000137 DAOQEW010000000
VERSION     DAOQEW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_10271_cov_8.002643"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1517)
                     /locus_tag="PLS35_13785"
     CDS             complement(39..1517)
                     /locus_tag="PLS35_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19788.1"
                     /translation="MPSISVLEQYDSNVFFTPKSQLAPGTKADDLITTITPQLNFMQS
                     NSLVNTNLSLGAMVQKFANNSALDNVGFNASGGLDFSQSVNRILPRMKSLRVMGTYRY
                     SPSAPAFGAGGFGGGVGGGGFGTGAVGIAGPVDSGLITQRIRTTMYSAGIADSYSLSP
                     TTDFQTTYTYSQISFGGSLAPTSTSSGQPNNTVFDTTTHAISAGPSSRISAIDTLTVR
                     YSFIRSSQAQFGDFTTHGGTVGWGRSWTKEWSSSVNGGMTLIEPIPDTSGIGGQRRIP
                     ATMFPIGGISLSYASGSSFLRKLGSEIQEATGTTGSGVSGGGFLPILAGMNVPGSIAS
                     PGSYRITLSYNLGVYPSFVQSAGPIYTHTIMLGSTAGITDRLTAQALFNFARSTFTSD
                     AIGTTFTTYGTTVSLNYLITPTFTARLSHQWLMFDNQATGLSAADLGFSKQVILLGFT
                     YAYAPRGDFFRSGAFWEGASGASSSGDAGTGKSGSGGVDTKK"
     gene            complement(1618..2241)
                     /locus_tag="PLS35_13790"
     CDS             complement(1618..2241)
                     /locus_tag="PLS35_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNI19789.1"
                     /translation="MKTFGCRWLRGGVCIVFSVLLTLPGCWIGNEQIDFNVTYIPPNE
                     FLLGPEDVLVVNVWRNQELSREVIIRPDGKISMPLVGDVQAAGMTSNVLAKRIADGLA
                     EFMSNPTVSVQVKEVNSYYVYVLGEVSKPGKVPLKSYATVLQGISLAGGFTTFASRNK
                     IHVLRVVPNGQGQPKQVQIPVPYEDILQGKNSEGNFFLKAGDVIVVP"
     gene            complement(2303..3004)
                     /locus_tag="PLS35_13795"
     CDS             complement(2303..3004)
                     /locus_tag="PLS35_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNI19790.1"
                     /translation="MKAKVVLSLMVLAGNCLNVAFAGPFDQAPKATLVAGAPAAAPPS
                     GMRHGGDGAEKSSLTVTPDYIMGPEDVLEITVWKNADLSKQVQVRPDGRISLPLIGDV
                     SAVGRTAAQLTEEISARLKSYMENPTVSILVREVNSYQIYVLGEVNAPGKYPLKSKTT
                     LLQAITIAHGFTQVAARNKIVVFRFGKDGEGLNKIKASYDDIVLRDGSDQNIELKPGD
                     QIVVPSETMVVLPSR"
     gene            3481..4092
                     /locus_tag="PLS35_13800"
     CDS             3481..4092
                     /locus_tag="PLS35_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNI19791.1"
                     /translation="MAQIAITSQETADNLADQRTGAGIVVLSSSMQLLHMNRQAAELS
                     KLINMAEKGGTPAKAAQGVLPSALTELCTEILKALQVRTEAKDWEQFEVKKIAGNPNQ
                     PVLLRGFGLPDRGGVQYARLVVTLEELGRRQQLNTDQAKERFQLTNREQSVVENLAKG
                     WTNKEIANALKITEQTVKEHIKHIMRKTNSTTRTGVLVQIFKS"
     assembly_gap    4120..4219
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4425..5555)
                     /gene="aepY"
                     /locus_tag="PLS35_13805"
     CDS             complement(4425..5555)
                     /gene="aepY"
                     /locus_tag="PLS35_13805"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HNI19792.1"
                     /translation="MIDPRKFVECLQSNSVEFYTGVPDSLLKEFCACLEQVPQAGRHV
                     ISANEGGAVALALGYHLATGKVPLVYLQNSGLGNIINPLLSLADAEVYSIPLLLVIGW
                     RGETGVHDEPQHKKQGRVMLAMLDAMEIPYSILGPEVNEAEGALSDALAYVQKHNAPF
                     ALVIKKGTFGAFTGDKRKTEIFELTREAAIQQVLEHLDERDVVVSTTGMASREVYEYR
                     MNKGQGHERDFLTVGGMGHASQIALGVALQRPSRSVYCLDGDGAVLMHMGALPITGTL
                     KPENFKHIVFNNGAHDSVGGQPTVGLEIEILEIAKACGYQLVVQAQTPQEVQAGLREL
                     KRSKVPCLLEIRIRRGARKDLGRPATTPVENKSAFMAFVQGG"
     gene            complement(5664..7298)
                     /gene="aepX"
                     /locus_tag="PLS35_13810"
     CDS             complement(5664..7298)
                     /gene="aepX"
                     /locus_tag="PLS35_13810"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HNI19793.1"
                     /translation="MNSRQPLTKTRQFRNLLLSEQLEFICEAHNGLSAKIVEEAGFPG
                     IWASGLSISAQFGVRDNNEASWTQVLEDLEFMSDATNIPILLDGDTGYGNFNNMQRLI
                     RKLEQRNIAAVCIEDKLFPKTNSFLKGDAQPMADMHEFCGKIKAGKDAQTDPDFCVIA
                     RVEAFICGWGLTEALRRAEAYHQAGADGILIHSALSVPDEILAFKREWGNRCPVVIVP
                     TKYYSTPTDVFRQHGFSMVIWANHMLRAAVSVMQKTARALKQSENLLSIEDKVAPVSE
                     IFRLQNAAELLEAEERYLPRGAEGTSAVVLAASRGEELGELTVEQPKTMVKIQGTPIL
                     SHIVDAYNAVGMKDILVVRGYKKEAVNLPNLTYIDNADYSQTGELASLSLALQSRKGV
                     FQPTIVSYGDVLFNKYIPQALCQEPDDCVIFVDSNWRDQTTYARLGGFVECTIPNSRK
                     AFNAKVYLKQLGDRIPTDATHGVWMGFLKLSSSAACDINNLLVEMLREPENRKAGIPH
                     LLQELLRREYPIRVLYTVGHWLDINSLDDVVQAGNF"
     gene            7942..8430
                     /locus_tag="PLS35_13815"
     CDS             7942..8430
                     /locus_tag="PLS35_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19794.1"
                     /translation="MRYHTLRQIILLLAGLTAVGCQSNPAVTSVRYDNVRFMDVWSTY
                     THCLSAEDSQVALQDSTKLRVVSRAQTTRSTLDTILPTSLKNMVSQPSSRLAVDVHAM
                     AASCSLHAGNLAMSAGELDVARSQFREVLQGHAESDYAYYTAQARERLTQLELTLQAA
                     LR"
     gene            complement(8468..9952)
                     /gene="glgA"
                     /locus_tag="PLS35_13820"
     CDS             complement(8468..9952)
                     /gene="glgA"
                     /locus_tag="PLS35_13820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNI19795.1"
                     /translation="MVASEVVPLAKTGGLADVVGALAVEFAKLGHDVCVMLPAYRQVD
                     ELGYEMTEGVRLSVPAGPSVMDVRVQEVFAPPLHVQGPGRLRVFVVRHDPYFSRSGLY
                     QEAGRDYLDNLDRFVCFCRTVMEMCIHLSEHEGWQTDLLHLHDWQAALCAVYLRTLYQ
                     GRPSLRRIRSVLTIHNLGYQGVFPAAQFSSTGLPLHLFTPAALEFYGQINVLKGGLVS
                     ADLLTTVSPTYSLEIQSSEYGCGLEGVITERKSVLSGIVNGIDTESWNPATDQYLVAP
                     YHASDLAGKARCKKALQRELGLRPLKCPLLGVIARLTSQKGIDLVIDVLPELMELDVQ
                     VVILGTGEPHYEQQVGELAQRYPGKIAVRNVFDEGLAHRIEAGADMFVMPSRYEPCGL
                     SQLYSLRYGTVPIVRKTGGLADTVVAYTPRAFKESRATGFSFTDTSSASLLTCLLLAL
                     SVYRKKAEWQRLVKTGMAQDLSWSRSAAIYLQLFRDVISRAGDR"
     gene            complement(10009..>10271)
                     /locus_tag="PLS35_13825"
     CDS             complement(10009..>10271)
                     /locus_tag="PLS35_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19796.1"
                     /translation="FGMPDGQLIPQARMALGESPQGWETGLAAGDAFFMAYADRGYYL
                     VFLDGVLVYHEAMSAEKVHAVGKTWKFESQFKTRLESSPGLK"
BASE COUNT         2601 a         2871 c         2409 g         2290 t
ORIGIN      
        1 tgctcgccaa taatcctctg gagccaaggt gcgcgtgttc atttctttgt atccactcct
       61 cctgatccag acttgccagt tcctgcatca ccagatgagg aagcaccaga agcaccttcc
      121 caaaaagcac ccgaacggaa gaaatcacca cgcggggcgt atgcgtatgt aaatcctaaa
      181 agaatgacct gcttagaaaa tcctaaatcg gccgcactca gaccggtagc ttgattgtca
      241 aacatcaacc attgatgact aagtcttgct gtaaatgtag gagtaatgag atagtttaac
      301 gatacggtcg tgccgtatgt ggtgaacgta gtgccgatag catcggaggt gaaagtgctt
      361 cgcgcaaaat tgaaaagcgc ttgagctgtc agtctgtcag taattcctgc agtactaccc
      421 aacataattg tatgagtgta aatgggacct gccgactgaa caaaactcgg ataaaccccg
      481 aggttatagg atagtgtaat tctataagat cctggtgatg ctatgctgcc aggaacgttc
      541 atcccggcaa gaatcgggag aaagcctcct ccgctcaccc cgctaccagt ggttccagta
      601 gcctcttgaa tttcactgcc caacttcctt agaaacgatg agcctgaggc ataactcaga
      661 ctaattccgc caatcgggaa catggttgcc ggaattctcc gttgccctcc tataccagaa
      721 gtatctggaa ttggctcaat gagagtcatt cctccgttga cggaagaact ccattcctta
      781 gtccaacttc ttccccagcc gactgtccct ccatgagtgg taaaatctcc aaattgcgcc
      841 tgcgacgatc tgatgaatga atatctcact gtcaaggtat caattgcaga tatgcgggag
      901 gacggtcccg cactgatcgc atgggtggtt gtatcaaaca ctgtattatt aggttgacct
      961 gaggacgtac tggtcggagc aagtgagccg ccaaaactaa tctgtgaata tgtgtacgtt
     1021 gtctgaaaat ccgtagtggg cgaaagagaa taagaatctg cgatcccagc actatacatg
     1081 gtggtcctga tgcgctgagt gatgagtcca gagtcaacag ggcctgcaat cccaacagca
     1141 ccggtaccaa acccccctcc tccgacacca ccaccgaacc ctccagcccc aaaggctggt
     1201 gcagacgggc tgtatcgata tgttcccatt actcgaagag atttcattcg aggcaaaatc
     1261 ctgttcactg actgcgagaa atctaacccg ccagaggcgt taaaccccac gttatcgagc
     1321 gcagaattgt ttgcaaattt ttggaccatt gccccaagtg aaagattggt attcacaaga
     1381 gaattgcttt gcataaaatt cagctgaggc gtaatcgtcg taatgagatc atcggccttt
     1441 gttcctgggg ccaattgaga ttttggagtg aagaacacgt tgctgtcgta ttgctcaaga
     1501 accgaaattg atggcacaac acgtatttgc tgagccgaag atggtgacgc gaaccagcat
     1561 gacacgaacg cgagtgtcaa acatattaaa gcgaatttcc ccatcctcaa aaactggtca
     1621 cggcacaaca atgacatcac cagccttgag gaaaaaattt ccctccgaat ttttaccttg
     1681 cagaatgtct tcatagggaa ccgggatctg gacctgcttt ggttgacctt ggccatttgg
     1741 aacgactcgc agtacatgga tcttatttct cgaggcaaac gtcgtaaatc cgccagccaa
     1801 tgatatgcct tgcaaaactg tagcatacga ctttaacgga accttacctg gcttcgagac
     1861 ctctcctaat acatatacgt aatagctatt aacctctttg acctggactg aaactgtggg
     1921 attggacatg aactcagcca atccatcagc aatacgcttc gccaacacat ttgaggtcat
     1981 gcctgccgct tgaacgtcac cgaccaacgg catggagatt ttcccatcag gtcgaataat
     2041 aacttcccgg gaaagctcct gatttctcca cacatttaca acaagaacgt cttcagggcc
     2101 taacaaaaac tcatttgggg gaatataggt gacgttaaag tctatttgct cgttgcctat
     2161 ccaacatcct gggagagtca gaagcacact aaaaacaata catacccccc ctcgtaacca
     2221 tctgcaaccg aaagttttca ctttcctaac tcctttggct cgtcgatacg aaatatcgtc
     2281 tgagcacctt gactaacgac aattatctgg acggcaatac aaccatagtt tcagacggta
     2341 caacgatctg atctcccggc ttgagttcga tgttttgatc tgagccatct cttaagacga
     2401 tatcgtcata actggcctta attttgttaa gcccctcgcc gtcttttccg aatctaaaga
     2461 ccacaatctt gttacgggcg gccacttggg taaatccatg agctatggtt atcgcttgca
     2521 acagagtggt cttacttttc agtgggtact tcccgggcgc attcacttcc cccaggacat
     2581 agatttggta gctgttcact tcccgaacca ggatggaaac ggtgggattc tccatataag
     2641 actttaagcg tgcagaaatt tcttccgtta gttgtgcagc ggtgcggcca acggctgaaa
     2701 cgtcaccgat caatggaaga gaaattcttc cgtcaggtct gacctggact tgcttcgaaa
     2761 ggtccgcatt tttccacacg gtaatttcca gaacatcctc aggacccata atgtaatccg
     2821 gcgtcacggt caaggaagac ttttctgctc catctccccc atgacgcatc cctgaaggag
     2881 gtgccgctgc aggagctccg gctaccagag tagccttggg ggcctgatca aacgggccgg
     2941 caaatgcaac gttgaggcaa tttccagcca ggaccattag gctcaatacg accttagctt
     3001 tcatgactta ttctcctgaa agaattccgg ttgcccatta cagcacagcg ctctgagacc
     3061 gttctcaagt tcagccgatt gcgatcaaat ccctgaatgg agttcccaat cgtcccggtc
     3121 acacacatcc ctggacagcc ccattcagcc cataacacat tgtttaaatt gcacaaacct
     3181 tggaagctat cgattttttt aggttaacgg gtgccttatg ctaaagaatc aaccgagagg
     3241 aatgggcaaa tacggacaaa tcaatgctgg ctctcatact aaagaagtcc gccaccgttt
     3301 tgaagtatag aatcaatggt ctcactggac aataccaatg atgtacggac cgcccccccg
     3361 aaggtatcat ttgactacct cgaaagtatt atgtagcatt actagtaacg ctgacacact
     3421 gtaggttatc agaaatcgac cagttgtcga ggaacattca gcatcatacg gagtattgcc
     3481 gtggctcaaa tcgctataac ctcgcaagaa accgcggaca acttggcaga tcaacgcacg
     3541 ggggcaggaa ttgtcgttct gtcgtcctcc atgcaactcc tacatatgaa ccggcaagcc
     3601 gctgaattat ctaagctcat taacatggca gagaaaggtg gtactccagc taaagcggct
     3661 caaggagtgc ttccatccgc tcttactgag ctctgtaccg aaatcctgaa agcgctccaa
     3721 gttcgcacag aggcaaagga ttgggagcaa ttcgaggtca aaaaaattgc gggcaacccg
     3781 aatcaacccg tgctcttgcg aggatttggt ctcccggata gaggtggtgt tcaatatgcg
     3841 cgcctagtgg tcacattgga agagttgggt cgtcggcaac aactcaatac tgaccaagct
     3901 aaggaacgct tccagctgac aaatcgggag caatcggtgg tcgaaaacct agcgaaagga
     3961 tggacgaata aagaaatagc aaacgcactg aaaatcaccg aacagacggt caaagaacac
     4021 atcaagcaca ttatgcgaaa aacgaactcc acgacgcgta caggagttct cgttcagatc
     4081 tttaaatcgt aagcccaatg cgtcaatcat ttccctaatn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnna gttctcgttc agatctttaa atcgtaagcc caatgcgtca
     4261 atcatttccc taatcccgcg acttccgata ctcacccacg cctgccctta atcataagat
     4321 ccctctgttc gccggtccag caggaaaata cctatcactc cagcgcaagg agcacgccca
     4381 gcgactttgc gcgaggttca gctcgcccct tattccccag tcgattagcc gccttggaca
     4441 aaagccatga aggcgctctt attctcgaca ggcgtggttg cagggcgccc cagatccttt
     4501 ctggctcctc ggcgtattcg aatctccaat agacatggaa ctttcgaccg ctttagctcc
     4561 cggagaccgg cttgtacctc ctgcggcgtc tgggcctgaa caaccagctg atagccacag
     4621 gccttggcga tctcaagaat ttcgatctcg agccccaccg ttggttgtcc acccactgaa
     4681 tcatgcgcac cattgttgaa aacaatatgc ttaaagtttt ccggcttcaa cgttccagta
     4741 atgggaagcg ctcccatgtg catgagaaca gcaccatccc catccagaca atagacggaa
     4801 cggctgggcc gttgaagggc aacacctaac gcgatctgag aagcatgccc cattccgccg
     4861 acggtgagaa aatcccgctc gtgcccctgc cctttattca ttctatattc atagacttca
     4921 cgggaggcca tgccggtggt cgacacgaca acatcacgtt cgtccaagtg ttccagcacc
     4981 tgctgaatcg cagcttctcg cgtcaattcg aaaatctccg ttttcctctt atcgccggtg
     5041 aaggcgccga aggtaccctt cttgatgaca agtgcgaacg gggcattatg cttctggaca
     5101 taggccaggg cgtcactcaa cgctccttca gcctcattca cttcagggcc taaaatggag
     5161 tacggaatct ccatagcgtc cagcatagca agcatgacac gcccctgctt cttgtgttga
     5221 ggctcgtcat gcacacccgt ctcgccccgc cagccgatca cgagcaacag gggtatcgaa
     5281 tacacttccg catcagccag ggacaacaaa ggattgatga tgttccccag ccccgaattc
     5341 tgtagataca ctaatggaac ttttccggtc gcgaggtgat agcccagcgc cagcgccaca
     5401 gcgccgccct cattggcact gatgacgtgc cgaccggctt gaggcacctg ctctaggcaa
     5461 gcacagaatt ccttgagcag cgaatcgggc acaccagtgt aaaactccac actgtttgac
     5521 tgtaaacatt cgacaaattt ccgaggatca atcatgcaca agttctccaa cctaagacgt
     5581 cccgatgaga cgatacagcg acgaacttca cagactggct gccacccgtt tcaaaacatc
     5641 accagcggac agaatgcgag ccatcaaaaa tttccggcct gcaccacatc atcgaggcta
     5701 ttgatatcga gccaatggcc aacggtatac agcacccgga taggatattc gcgccgcaac
     5761 aattcctgga gcaagtgagg aataccggcc ttgcgattct ccggctccct cagcatctcg
     5821 acgagaagat tgttgatatc acaggctgcg gacgaagaga gtttaagaaa tcccatccac
     5881 actccgtggg tggcatcagt cgggatccga tctcctaact gcttcagata gactttcgca
     5941 ttgaaggcct tccgtgaatt gggaatggtg cattcgacaa agcctcccaa acgcgcatac
     6001 gtcgtttggt cgcgccagtt gctatcgacg aatatcacac agtcatccgg ctcttggcac
     6061 agcgcctgcg ggatatactt gttgaacaac acatcgccat acgaaacgat agtcggctga
     6121 aaaactccct tcctggattg caacgccagc gataatgagg ctaactcgcc agtttgcgaa
     6181 tagtccgcat tgtcgatata ggtcaggttg ggaaggttga ccgcttcttt tttgtacccc
     6241 cggaccacca aaatatcctt catgcccacg gcattgtacg catccacgat atgagacaag
     6301 atcggtgtgc cctgaatttt gaccatggtt ttcggctgct caaccgtcag ctcacccagt
     6361 tcttcacctc gggatgcagc caacaccacc gctgacgtgc cctcggcgcc acgagggaga
     6421 taccgctcct cagcctccag cagttcggcg gcattctgca aacggaagat ttctgaaaca
     6481 ggggccacct tgtcttcgat ggacaggaga ttttcactct gcttcaaggc ccgtgcagtt
     6541 ttctgcatga ccgacaccgc ggcacgaagc atgtggttgg cccagatcac catcgagaag
     6601 ccgtgctggc gaaagacatc cgttggcgtg gaataatact tggtaggaac gatgacaacg
     6661 ggacaccgat ttccccattc tcgcttaaag gccaggatct catcaggaac agacagggca
     6721 ctatggatga gaataccgtc agccccggcc tggtgatacg cttcagcgcg gcgcaatgcc
     6781 tccgtcaaac cccagccaca gataaaggct tccactcgtg cgatgacgca gaaatcggga
     6841 tcggtttggg catctttccc ggctttgatt ttgccgcaga attcatgcat atcggccatc
     6901 ggctgcgcat cgccctttaa aaagctgttg gtcttgggaa agagcttgtc ttcgatgcac
     6961 acggccgcaa tgttgcgctg ttccagctta cggatgaggc gctgcatgtt gttaaaattg
     7021 ccgtaccccg tatccccgtc gagaagaatc ggaatgttag tggcatcgga catgaattcc
     7081 agatcctcga gcacctgagt ccagctcgct tcgttgttgt cacgcacgcc aaactgagcc
     7141 gagattgaaa gaccgctggc ccagataccc gggaaccccg cctcctctac aattttggca
     7201 ctgagtccgt tgtgcgcttc acaaataaac tccagttgct cggagaggag caaatttcga
     7261 aactggcggg tcttggttaa cggttgtctc gaattcattg gcgaaggaac ctttcctgag
     7321 gaagttctgt ctcgttcgtc gacgaagata ttcttcgtac catatgaaag accatcaagt
     7381 cgcaagaata ttcgtcgtca atgattcgcg aatcgcaccc attctttctg agagatgaat
     7441 gagtcgagaa cgcatatgtc ggcctagagt aaggtcggca catttccgtc gcgatggcca
     7501 gatagtagcc gagcctgcag ccctggcgct accgattctt gacagacaag caacgatacc
     7561 ccataggcct gccttgacgc ctctgtgcat cttcactgac gaattgggat acactcggtg
     7621 agttcaaacc gtccgcagtg tccaggacgc gatcgacagc accagccatc ggccggaaag
     7681 cgacacttcc cgcaccctcc aggatcttcc tcacttcgtg agcctcggcc tgctcaacag
     7741 cccttcatct ggcctgtgcc gcgtggcctc atcatccgtg tcgtattccc cacagtgaac
     7801 ttaaattgag acagcaccgc ttcagatgat gacaactggc cgcaaataat tcaatgactt
     7861 acatgtgcca cacgtggcgc aagacttgct cattccgagg acaagacaag agtcgagggc
     7921 agatcgacaa agactggcac catgcgatat cacaccttac gccaaatcat cctcttactc
     7981 gcgggcctca cagccgtagg ctgccaatcc aatcctgccg tcacctcagt gcggtacgac
     8041 aatgtccgtt tcatggatgt atggagcacc tacactcatt gcctctcagc ggaggacagc
     8101 caagtagcct tgcaggactc caccaagtta cgagtcgtca gccgagctca aaccacgcgg
     8161 tcgacgctcg ataccatcct gcctaccagc ctgaaaaata tggtcagtca gccgtcctcg
     8221 cgcttggcgg tggatgttca cgccatggcc gcatcctgca gtttacatgc ggggaacctg
     8281 gccatgtcgg ccggggagtt agacgtggcg aggagtcagt ttcgagaggt attacagggc
     8341 cacgcggaat cggattatgc ctactacact gcacaggcac gggagcggct cactcagctg
     8401 gaactgacac tccaagctgc gcttcgataa ccttcctcat ttcttcctcg ccccaacgca
     8461 acgagtttta tcgatcgcct gctcgagata tcacatctct gaataattgt agatagatcg
     8521 ctgctgaccg actccatgag agatcctgtg ccattccagt cttcacgaga cgctgccact
     8581 cggctttctt tcgatacaca gacaatgcga gtaacaggca ggtaaggagc gacgcagaac
     8641 tggtatccgt aaaactgaaa ccggtcgccc gggattcctt aaaggctcgc ggggtataag
     8701 ccacgaccgt atcggccaac ccgccggtct ttctcacaat aggaaccgtc ccataacgta
     8761 aactatacag ctgactgaga ccgcaaggct cgtagcggga tggcatgaca aacatatccg
     8821 ccccggcttc aatccggtga gccaatcctt catcgaacac attccgcacc gcaattttcc
     8881 ccggataacg ctgggccagt tcacccacct gttgctcata gtgcggctcg ccagtgccga
     8941 gaatcacaac ttgcacatcg agctccatga gctcgggcag cacatcgatg acgagatcga
     9001 tgcctttttg gctggtcagc ctggcgatca ctccaagcag cgggcacttc agcggacgca
     9061 gtcccaattc ccgttgaagc gccttcttgc agcgagcctt ccctgcgagg tcggatgcat
     9121 gatagggagc caccagatat tggtccgtgg ccgggttcca actctccgta tcgattccgt
     9181 tcacgatccc agacagcacg ctcttgcgct cagtgatcac cccttccaga ccacatccat
     9241 attccgagct ttgaatttcc aggctgtagg tggggctgac cgtggtcagg agatccgcgg
     9301 agaccagccc gcccttcaac acgttgatct gtccatagaa ctcgagggct gcaggcgtga
     9361 acagatgcaa cggcagtccc gtcgaagaaa actgcgctgc gggaaacacc ccctgatatc
     9421 caagattgtg aatcgtcaag acgctacgga tacggcgcaa tgagggccgt ccttgataca
     9481 gcgtccgtag atagaccgca cagagggcag cttgccaatc atgaaggtgc aggagatccg
     9541 tctgccatcc ttcgtgctcg ctgagatgaa tgcacatctc catgacggtc cggcagaagc
     9601 agacaaaccg atccaggttg tcgagatagt cgcggccagc ctcttgatac agaccagaac
     9661 gagaaaaata cggatcatgc cgaacgacaa agacacgcag ccgcccaggt ccctgtacgt
     9721 gcagcggcgg tgcaaacact tcctgcacgc gcacatccat cacgctaggc ccggcgggaa
     9781 cagacaaacg gacgccctcc gtcatctcat accccagctc gtcgacctgt cgatacgccg
     9841 gcaacatcac gcagacatcg tgtcccagct tggcaaattc gactgccagc gcgccgacaa
     9901 catcagccag gccacccgtt ttcgccaacg gcacgacctc tgatgccacc atgcaaatct
     9961 tcaaggaagt ttgatccgga gtcagattca tggagaaaac acgaccgcct atttcagacc
    10021 gggtgatgat tcaagccgcg tcttgaactg ggactcgaat ttccaagtct tgccgaccgc
    10081 atgcactttt tcggccgaca tggcctcatg gtatacgagt accccgtcaa gaaagacgag
    10141 gtagtatccg cgatcagcat aggccatgaa gaaggcatca cccgcagcca agcctgtttc
    10201 ccatccctgg ggtgactcgc caagcgccat gcgcgcctga ggaatcagtt gaccatcggg
    10261 catgccgaaa a
//
LOCUS       DAOQEW010000138        10225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2049_length_10225_cov_8.335202, whole genome shotgun sequence.
ACCESSION   DAOQEW010000138 DAOQEW010000000
VERSION     DAOQEW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_10225_cov_8.335202"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..658
                     /locus_tag="PLS35_13830"
     CDS             29..658
                     /locus_tag="PLS35_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNI19797.1"
                     /translation="MAFSTNRLFKYQRGMRRRIGMLRAKNIDSMEFAVDFMMQERVDS
                     YFRIEPALEEVERSLGQIEDELDLVKDVSGAIRLESRLEFVEDRWDELDSEIRERPRR
                     RRRKINLADFLKAAGGEGNPAGTSSEVTNAYDAYQLLGLEFGTPLADVTTAFRQRAKE
                     LHPDARNGDRSSEPELRRILEAYQFLKEYLSLSNTEPPISRGAEYRPTE"
     gene            662..1867
                     /gene="rnd"
                     /locus_tag="PLS35_13835"
     CDS             662..1867
                     /gene="rnd"
                     /locus_tag="PLS35_13835"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNI19798.1"
                     /translation="MATLDPDQTFITSDHMLDRLCDQLANSAVIAIDTEFMGEDHFIP
                     RLELIQVAAEGVAAVIDFPAVQDSAPMVRFWELVCDARIEKVLHAGRQDLELFAHHAG
                     RLPKPFFDTQIAAAMVGYGAQTAYANLVQRVQGVKLDKAHTFTNWSQRPLSKDQLVYA
                     LDDVTFLLPIHRHLRQKLSVMGRLDWVDEEFSRLEVSLGAQARDPQERYQRIRGWDSL
                     KPRAAGVLRDLAAWREGEARRRNVPRGRVMRDEVLVQLARQTPRTLDQLRGMRGMYSS
                     EVERNGQALLATMQQALARPESEWPEVPRDRKPDPESTGVLELLQAVLKSRAAMENIA
                     PTLIATTGDLQALVERTSPVEEVDIPVLRGWRRQLVGETLLSVLSGNLKVWIDPQIGK
                     LRFGHLDHP"
     gene            complement(1901..2143)
                     /locus_tag="PLS35_13840"
     CDS             complement(1901..2143)
                     /locus_tag="PLS35_13840"
                     /inference="COORDINATES: protein motif:HMM:NF040329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19799.1"
                     /translation="MLAFDSVDRVSLLGRVLQRLNLRFPSLFVLFAVLTLVDVIVPDV
                     IPLADEIGFALLTLLLGMWKDRRHQPRQRTAGKAFR"
     gene            complement(2204..2554)
                     /gene="arsC"
                     /locus_tag="PLS35_13845"
     CDS             complement(2204..2554)
                     /gene="arsC"
                     /locus_tag="PLS35_13845"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNI19800.1"
                     /translation="MAEITIYQKPTCSTCREAVRLVRESGQPFTAVNYYEQPFTKSRL
                     KELLKKAGLSPRDVLRTKEDLYKSLKLADSTLSDDHLLDQMIAHPDLIQRPIVVKGDK
                     VMLARPADTVTELL"
     gene            2655..3029
                     /locus_tag="PLS35_13850"
     CDS             2655..3029
                     /locus_tag="PLS35_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19801.1"
                     /translation="MPIFGTDTSDQTGKFSCATATQRTLKDLRTKRKGQPVFVVGHRL
                     ERKGKEATFEVFNDRLAIIRFEDDVRLGYDPFELLLPTEIDDKGVAYFEIRTCRLCEQ
                     FFPLTADECDEEEERTTCPECA"
     gene            complement(3087..3470)
                     /locus_tag="PLS35_13855"
     CDS             complement(3087..3470)
                     /locus_tag="PLS35_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19802.1"
                     /translation="MGRFRSLALGVLMVIGLNVPAWADDEQVFFAVVSDVPKDKTRIS
                     AKVLQDGASSDIKLIPSESVVANPVWRTLEVCHALKIEGMKIGDGLKVMSARVLDASM
                     LPMGLQGFAGDCLIKKAVEIAPLAE"
     gene            3941..5074
                     /locus_tag="PLS35_13860"
     CDS             3941..5074
                     /locus_tag="PLS35_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNI19803.1"
                     /translation="MPHDFMPGLAGVPAAKSAISDVDGIRGVLEYRGIRVEELCQHSS
                     FLETSYLLLFGRLPTATELTQWVDDVTHHRRIKFRIVDLLKVMPEHGHPMDALQAAVA
                     ALGMFYPGRNVKDVENNYWSAVRLIAKLPTIVAAWARLRRGDEYIPPRDDLPFADNFL
                     YMLTETVPHPLWSAVFDDCLILHAEHTMNASTFAGMVTASTLADPYTVVASSIGALKG
                     PLHGGANEEVVTMLKEIGTPAQARAAVEARLGGKNKLMGFGHRVYKVKDPRATVLQDL
                     CMRLFNVCGTSPLYEVAVAVEQLASERLQGKGIYPNVDFYSGIIYDKMGIEVDLFTPL
                     FAMARVSGWLAHWLEQLRENKLYRPDQIYSGEHDRHYVPIDRR"
     gene            5364..5819
                     /locus_tag="PLS35_13865"
     CDS             5364..5819
                     /locus_tag="PLS35_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNI19804.1"
                     /translation="MAIVRWDPFRELEEMSDRLNRMITRPGAAQTATQGKEVMTVADW
                     APTVDISETEAEYAIKAELPEVKKEDVKVTVEDGVLTLQGERKQEKEDKGKKYHRIER
                     SYGRFVRSFTLPDTVDESKVKAEYTDGVLHLHLPKSEKAKPKQIDVKIA"
     gene            6026..7483
                     /locus_tag="PLS35_13870"
     CDS             6026..7483
                     /locus_tag="PLS35_13870"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNI19805.1"
                     /translation="MMKNASRTPKSTASATTATRMERDTMGELPVPSNAYYGVQTARA
                     IENFPISSLRIPRAMIRAMGLIKRAAASVNHSLKLLDKKPADAIIRAATEVVDGGLDA
                     EFPVDIFQTGSGTSTNMNTNEVISNRATELLGGARGSKLVHPNDHVNLGQSSNDVIPT
                     AIHIAASETIQRQLLPALTQLHKALSGKAREFDKIVKIGRTHLQDATPVRLGQEFGGY
                     ARQIELGIARMKRAQAALSEVALGGTAVGTGLNCHPKFAGKVMALISKETGCSFKEAV
                     NHFEGQSAQDSLVEASGELRTLAVSLMKIANDIRWLGSGPRCGLGEINLPETQPGSSI
                     MPGKVNPVIAESVTMVCAQVIGNDVTITVGGQAANFELIVMLPVMAYNLLQSIELLST
                     ASTNFAVKCIAGIKANEERCKSLIEESLAMCTALAPVIGYEAAAKLAKDAYKSGKTVR
                     EVAREQNVLPEKRLNQLLDPWRMTQAGGPVGSAGG"
     gene            7666..9036
                     /locus_tag="PLS35_13875"
     CDS             7666..9036
                     /locus_tag="PLS35_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI19806.1"
                     /translation="MSATGSETSGHVVVVVGAGPAGMALAKKMADAGHEVIILNRDIK
                     FGGLAEYGIFPSKLKLRGGLKKQYWDMLEQPNVHYFGNVSVGQGKDLTVEELRGLGAS
                     AVAFSIGAQGTKAIGVEGDSAKGVFHAKDVVYHFNRLPGFGDRPFDMGKHVAIIGVGD
                     VMVDIAHWLIRYKKVERVTAIARRGPVERKYNPKEIRAVCSNMDHEGIAQEFARIKDR
                     LAAVGQNADEVLANLTAEFTKCEPTGSSSKMGFRFLASPKRVLVDANNRVRALEMEEN
                     KLEPKGEDTAAVGLKQLYEFPVDSVVFAVGDRVDETVGLPYKNGVYVTNPTKTANDPD
                     DSLFQAYDEKSGQVVDGVFLAGWARKASEGLVGIAKRDGDWCAEVISRYLTTKSVQSV
                     SAVDQVLSKLQAILKERKSKPVDLEGLKVLDTVEKAHAASPEAIGEFKFASNADMLAH
                     IERGRA"
     gene            complement(9052..9912)
                     /locus_tag="PLS35_13880"
     CDS             complement(9052..9912)
                     /locus_tag="PLS35_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNI19807.1"
                     /translation="MKSKSIGILTKPKFPEVKATVQAVVSWLRSRNIDVLLDTTSTTL
                     LGEQGGFQKTQLASKADVLLVLGGDGTMLNAARLAGERGIPILGVNMGGLGFLTEVVL
                     ENLYPSLERVFANDFVLDERLMLRTHVHRHGETVARGVVLNDVVISKGTLARMIELKI
                     AIQGQFVTNLRGDGLIISTPTGSTAYSLSAGGPIINPAVPSLILTPICPHTLTHRPLI
                     VPASAQIEVVLTSKDDGAMATLDGQVGVALSQGDTVEIHASEHLTRLIRFPESSYYEV
                     LREKLKWGDG"
BASE COUNT         2309 a         2996 c         2914 g         2006 t
ORIGIN      
        1 cctgcaaggt cacgcctaac gaggactcgt ggcattttca accaatcgcc tgttcaaata
       61 tcagcggggc atgcggcgcc ggatcggcat gctgcgcgcc aagaatatcg acagcatgga
      121 gtttgccgtc gatttcatga tgcaggaacg ggtcgatagc tatttccgca tcgaaccggc
      181 cttggaagaa gtcgaacgtt cgctgggaca gatcgaagac gaattggacc tggtgaagga
      241 tgtgtccggg gccattcgtt tggaatcacg cctggaattc gtcgaagatc gctgggatga
      301 gcttgacagc gagatacgtg aacgcccgcg acggcgccgg cgaaaaatca atctcgccga
      361 tttcctcaaa gccgccggag gggaggggaa tcccgccggt acgtccagcg aagtcacgaa
      421 cgcctacgac gcctatcagt tgctgggcct ggaattcggc acgccgctcg cagacgtcac
      481 cacggcgttt cgtcaacgcg ccaaggaatt gcatcccgat gcccggaacg gcgaccgcag
      541 ttccgagccc gaactccggc gcattctcga agcctatcag ttcctcaaag aatatctgag
      601 cttgagcaac accgaaccgc cgatttcacg cggcgcggaa tatcgtccca ccgagtaacc
      661 catggccaca ttggatccgg atcagacctt catcacctca gaccatatgc tcgaccggtt
      721 atgcgaccag ctggccaaca gcgcggtgat cgccatcgac accgagttca tgggtgaaga
      781 ccatttcatt ccgcgcctgg aattgatcca ggttgcggcg gaaggggtgg ctgccgtcat
      841 cgactttcct gccgtgcagg acagcgcgcc gatggtccgc ttctgggaac tcgtctgtga
      901 tgcgcgcatc gagaaggtgc ttcatgcagg tcggcaggat ctcgaactgt tcgcgcacca
      961 tgccggccga ttgccgaagc cgtttttcga cacgcaaatt gccgccgcga tggtcgggta
     1021 cggtgcgcag accgcttacg ccaatctggt gcagcgggtg cagggggtga aactcgataa
     1081 agctcacacc ttcaccaact ggagtcaacg accgttgagc aaggaccaat tggtctacgc
     1141 cctcgatgac gtgaccttct tgctgcctat ccatcggcat ctccgtcaga aactgtcggt
     1201 gatgggccgt ttggactggg tggacgaaga attctcccgc ctggaggtct cgctcggagc
     1261 ccaggctcgc gacccgcagg agcggtacca gcgtattcgc gggtgggaca gcctcaagcc
     1321 gcgcgccgcc ggcgtgttgc gcgacctggc cgcctggcgg gaaggggaag cccgccgccg
     1381 caacgtccca cgagggcggg tgatgcgcga tgaagtgttg gtgcagttgg cccgtcaaac
     1441 cccgcgcacg ctcgatcaac ttcgcggtat gcgcggcatg tattcctcgg aagtcgaacg
     1501 caacgggcag gccctgcttg ccacgatgca gcaggccttg gccagaccgg aatccgagtg
     1561 gccggaagtg ccacgcgacc ggaaaccgga tcctgaatcc accggggtgc tggaactgct
     1621 gcaggccgtg ctgaaatccc gcgctgccat ggagaacatc gccccgactc tcatcgccac
     1681 gacgggcgat ttgcaggcct tggtggaacg gacgtcgccg gtggaagaag ttgacattcc
     1741 ggtcctgcga ggatggcggc gccaactggt gggggaaacg ctgctcagcg tcttatccgg
     1801 caatctcaaa gtctggatcg acccgcaaat cggcaaactc cgcttcggac atttggacca
     1861 tccgtagtcg agctcacctc gaccgaacct tctctgcccc tcaccggaat gccttgccag
     1921 ccgttcgctg tcgcggctga tgccgacgat ccttccacat ccccaacaac agcgtcagca
     1981 atgcgaaccc gatttcgtcg gccaacggaa tcacatccgg gacaatcaca tccaccaggg
     2041 tgagaacggc gaacaacaca aagagggatg ggaagcgaag attgagccgc tgcagaaccc
     2101 gtcccagcag tgacacccga tctacggaat cgaaagccaa catctctgtc cctccgccaa
     2161 tccgctaccc acgccccgcc ttgcatcgtc tctctcggga accttagagc aattcggtga
     2221 cggtgtcagc cgggcgcgcc agcatgacct tgtctccttt gaccacgatg ggccgctgaa
     2281 tcaaatccgg atgtgcgatc atctgatcca gtaagtgatc gtcgctcaac gtcgaatccg
     2341 caagcttcaa cgacttgtag agatcttctt ttgtgcgcaa gacatcgcgc ggggacaggc
     2401 cggctttctt cagcagctct ttgagccggc tcttcgtaaa cggttgttcg tagtagttga
     2461 ccgccgtaaa tggctgcccg ctctcccgca ccagacgcac cgcctctcga caggttgaac
     2521 aggtgggttt ctgatagatc gtgatctccg ccatgccgtt aacctttctc cttcatgagg
     2581 gttcccttga cgcaaatttt tcacctgtgt tacatggaca cacaagattc atatcaacgc
     2641 gattgtgatt accgatgccc atctttggaa ccgatacgag cgaccagacc ggcaaattct
     2701 cctgtgccac cgcgacacag cgcaccttga aagatttgcg caccaaacgc aagggccaac
     2761 cggtgtttgt cgtgggacat cggctggagc gtaaggggaa agaagccacg tttgaggtct
     2821 tcaatgaccg cttggccatc atccggttcg aagatgacgt ccggctgggc tacgatccgt
     2881 tcgaactgct gcttcccacg gagatcgatg acaagggcgt ggcctatttc gagatccgca
     2941 cctgccggct ctgcgagcag tttttccccc tcacggcgga tgaatgtgac gaggaagaag
     3001 aacggacaac ctgcccggag tgcgcctgac aagcaggata cgactcagga cgcacaacta
     3061 catattcccg acttacgata gacttgtcac tctgccagcg gcgcaatctc gaccgctttc
     3121 ttgatcaggc aatctcccgc gaacccttgc agtcccatcg gcaacatgct ggcatccagc
     3181 acccgggctg acatcacctt caatccgtcg ccgattttca taccttcgat cttgagtgcg
     3241 tgacagactt ccagcgtgcg ccatacggga ttggcgacga ccgactcgct cggaatcagt
     3301 ttgatgtcgg aggaagcgcc gtcctgaagg accttcgcag aaatacgcgt cttatctttc
     3361 ggcacatcac tgacgaccgc gaaaaagacc tgctcatcat cagcccaggc cgggacattc
     3421 agaccaatga ccattagcac tcccagcgcg agcgaccgaa accggcccat gccttacctc
     3481 gttgttgcgg gtggagcctg tgtggacgat tcattcctct cgcctgcagg aagacgtggg
     3541 gaattgtcgg actgagatcc ggcgccggga ccgccatgac tcagggtgct gcgtcctgcc
     3601 cgtggggatc ccggggcgac ggcggaacgt tcttcagtcg gcgggtctcc gatccgcaac
     3661 atccacagag cgacgcctgc aaccaacagt ccgaccagca gaagaatgcg tacgaatgga
     3721 gtcaccgtgt tctccgcggt cgaagtcctc ttcgtttcga ggtagtatgc caagcgaatc
     3781 cattcaattc aaggtgaagg ctgagtcaat gccgtcgatc gtgataagct gaggccagac
     3841 gaccgtcact caggaagccc ggacacgagg gccgctcatg gaaccgatcg catcacgcgt
     3901 gatgccccat tcggattgtt gaagatagga gacagaatct atgccgcacg atttcatgcc
     3961 gggtctggcc ggtgttcctg ccgcaaaatc agcgatcagc gatgtggatg gaatacgagg
     4021 cgtccttgaa tatcgcggaa tccgggtcga ggagctgtgt cagcactcgt cctttctcga
     4081 aacgagttac ctcctgttgt tcggacggct accgactgcg acggaactca cgcagtgggt
     4141 ggatgatgtc acacaccacc ggcggatcaa gtttcgtatt gtagacctgc tgaaggtcat
     4201 gcctgaacat gggcatccca tggacgcgtt gcaggccgcg gtcgcggcgc tgggcatgtt
     4261 ttacccgggc cgcaacgtca aggacgttga aaacaactac tggtcggcgg tgcgactcat
     4321 cgcgaagctg cctactatcg tagccgcctg ggcgcggttg cgacggggag acgaatatat
     4381 tccgccgcgc gacgatctgc cctttgccga caactttctc tacatgctga cggaaaccgt
     4441 tccgcacccg ttgtggagcg cggttttcga tgactgcctg atccttcatg ccgaacacac
     4501 catgaatgcc tccaccttcg ccggcatggt caccgcgtcg acactcgcgg acccctatac
     4561 cgtcgtggcc tcgtcgatcg gcgcgctcaa gggcccgtta cacgggggcg ccaatgaaga
     4621 agtggtgacg atgttgaagg agatcggcac gccggcgcag gctcgcgcgg cggtggaagc
     4681 ccggctcggc gggaaaaaca aactcatggg attcggccat cgcgtgtaca aggtgaaaga
     4741 tccgcgcgcc accgtgctac aggatctttg catgcggctg ttcaatgtct gcgggacttc
     4801 gccgctctac gaagtcgcag tagccgtgga acagctggcc agcgaacgat tacaaggaaa
     4861 gggcatttac ccgaacgtcg atttctattc gggcatcatt tacgacaaga tgggaatcga
     4921 ggtcgatctg tttacgccgc tcttcgccat ggcgcgggtg agcggttggc tggcgcattg
     4981 gctggaacaa ctgcgggaaa acaaactcta ccggcccgat cagatctatt ccggggaaca
     5041 tgaccggcat tacgtgccga tcgatcggcg ataaaccgac ggcgaactat tcggtacgac
     5101 cgatgaacca ccatacgacc atggacgcaa ccatcaagat accgccgagc aatgcgtagg
     5161 aaatgaattc caacatggtg atcgtctcca atatgaaggg ggctaataaa taatgccgag
     5221 ggctgcaatg tcaagagccc gctcatgaca attactcttc agggcacacc cttgacttca
     5281 ccggcgtgga gattaccttc cttgtattaa gtaggatggc tcaagtgaac accagcaacg
     5341 cattcacaca aggaggttcc accatggcga tcgtacgttg ggatccgttc cgggaattgg
     5401 aagaaatgtc tgaccggttg aaccggatga ttacacggcc gggcgcagct caaaccgcta
     5461 ctcagggcaa ggaagtgatg accgtcgctg attgggcgcc gacggtcgac atcagcgaga
     5521 ccgaagccga atatgccatt aaggccgagc ttccagaggt caaaaaggaa gatgtgaagg
     5581 tcacggtcga agacggtgtc ctcacgttgc agggcgagcg gaagcaggaa aaggaagaca
     5641 aggggaaaaa gtaccatcgg atcgaacggt catatggacg ctttgtccga agcttcacgc
     5701 ttcccgacac cgtggacgag agtaaggtga aggcggaata taccgatggt gtgctccacc
     5761 tgcatctgcc aaaatcagaa aaggcgaaac cgaaacaaat cgacgtcaaa atcgcctaag
     5821 acaacgaacc ccggtgagag agcaaggccc gccttccggc gggccttttc tttttcagtt
     5881 cgtccctgaa cttcttcacc ccatacaaca actccctgag cgccccctcg ttcctctctc
     5941 caaggccatg cttgtgcttc agaagggcga gcgtttatta tgcgcggttc aagcagctca
     6001 accgccagtc attcgaggag attcaatgat gaagaacgct tcgcgcacgc ccaagagcac
     6061 tgcatcagcg acaaccgcca cgcgcatgga acgagacacc atgggagaac ttccggttcc
     6121 ctccaatgcg tattatggcg tgcaaacggc ccgcgcgatt gaaaactttc cgatcagttc
     6181 gctacgcatt ccccgcgcca tgattcgggc gatgggactc attaagcgcg ccgccgcctc
     6241 cgtcaatcac tcgctgaagt tgctcgataa aaaaccggcg gacgccatca ttcgcgccgc
     6301 gaccgaagtc gttgacggcg ggctggacgc cgaatttccg gttgatattt ttcagaccgg
     6361 ctcgggcacg tcgacgaaca tgaatacgaa cgaagtcatt tcgaaccggg cgaccgaact
     6421 gctcggcggc gccagaggca gcaaactggt gcacccgaac gaccacgtga acctgggaca
     6481 atcgagcaat gacgtcattc caaccgccat tcatattgcc gcttcggaaa ccatccagcg
     6541 gcagcttctc cctgccctga cccaactcca caaggcactc agcggcaaag cccgcgagtt
     6601 cgacaagatc gtcaagatcg gccgcacgca tttgcaggat gcaacaccgg ttcgcctagg
     6661 acaagagttc ggcggatacg ccagacaaat cgaactgggg attgccagaa tgaagcgggc
     6721 ccaggcggcc ttgagcgaag tcgccctcgg aggcaccgcc gtcggcaccg gactcaactg
     6781 tcacccgaaa tttgccggca aggtgatggc gctcatttcg aaagaaaccg gctgtagctt
     6841 caaggaggcg gtgaaccact tcgaaggtca gtccgctcag gattcgctgg tcgaggcgag
     6901 cggagagtta cgcaccctcg ccgtcagcct gatgaagatt gccaacgaca tccgctggct
     6961 cgggtccggt ccgcgctgcg gcctcggcga aatcaatctt ccggaaaccc agcccggctc
     7021 gtccatcatg cccggcaaag tgaatcccgt gattgcggaa tccgtcacca tggtctgcgc
     7081 ccaggtcatc ggcaatgacg tcaccatcac cgtcggaggg caggccgcga actttgaact
     7141 gatcgtgatg ttgccggtca tggcctacaa cctcttgcag tccatcgaac tgctctcgac
     7201 agcctcgacc aactttgccg tgaagtgcat cgcgggcatc aaggcgaacg aagagcgctg
     7261 caagagcctc attgaggaga gccttgcgat gtgcacagcg ctcgcgccgg tcatcggtta
     7321 cgaagccgcc gccaaactgg ccaaggacgc ctacaagtcc ggcaagaccg tccgtgaggt
     7381 ggcgcgcgaa caaaacgtgt tgcctgaaaa gcgactcaac caacttctcg atccctggcg
     7441 tatgacccag gccggtggcc cggtcggcag cgccggaggg taggcgacga gggggttaca
     7501 ccagcctccc ccaaagccgt cgacatcggg gcagctgaac cgcctacgtg attttgacag
     7561 cccgacaacc cctgtgctac cgtgccaaaa aattttgggt tctttggccg tctgacggtc
     7621 gaggtaatca cgacctattt atagatatac attgaagggt agataatgag tgcaaccggt
     7681 tccgagacaa gcgggcatgt agttgttgtt gtgggcgcag gtcctgccgg aatggccctg
     7741 gccaagaaaa tggccgatgc cggccatgaa gtcatcatcc tcaatcgtga catcaagttt
     7801 ggcgggttgg cggagtacgg gattttcccc agcaagctca aactgcgcgg cggtctgaag
     7861 aaacaatatt gggacatgct ggaacagccg aatgtgcact acttcggcaa tgtgtcagtc
     7921 gggcagggca aagacctcac ggtggaggaa ctgcggggat tgggtgccag tgccgtggcc
     7981 ttctccatcg gcgcccaggg cacaaaagcc atcggcgtgg aaggtgactc cgccaagggc
     8041 gtgttccacg ccaaggatgt ggtctaccac ttcaatcgcc tgcctggttt cggcgaccgt
     8101 ccgttcgata tgggcaaaca tgtggccatc atcggagtag gcgacgtcat ggtcgacatc
     8161 gcacactggc tcattcgcta caagaaggta gaacgggtga cggctattgc tcgtcgcggg
     8221 cccgtggagc gcaaatataa tcccaaggaa attcgagccg tctgctcgaa tatggatcac
     8281 gaaggcatcg ctcaagaatt cgcacggatc aaggaccgcc tggccgcagt cggacagaat
     8341 gccgacgaag tcctggcaaa tttgaccgcg gaatttacga agtgcgaacc gacgggaagc
     8401 tcctccaaaa tgggattccg gtttcttgcg tcaccgaaac gggtgctcgt cgatgccaac
     8461 aaccgcgtgc gcgccctcga gatggaagaa aacaaattgg aaccgaaggg cgaagacacc
     8521 gccgcggtcg gactcaagca gctgtatgag ttcccggtag acagtgtcgt ctttgccgta
     8581 ggtgatcggg tcgatgagac ggttggcttg ccctacaaga acggcgtgta tgtcacgaac
     8641 ccgaccaaga ccgccaatga tccggacgat tcgttattcc aagcctatga tgaaaaatca
     8701 gggcaggtgg tcgacggggt gtttctcgcc gggtgggcgc gcaaagccag cgaagggctc
     8761 gtcggaattg ccaagcggga cggggattgg tgtgcggaag tcatctcgcg ctacctcacg
     8821 accaagtcgg tccaatcagt cagtgcagtc gaccaggtcc tgtcgaaatt gcaggcgatc
     8881 ctgaaggaac gaaagagtaa gccggtggac ctcgaaggcc tcaaggtgct ggataccgtg
     8941 gagaaggctc acgcggcatc acctgaagcc atcggagaat tcaagtttgc ttcgaacgcc
     9001 gatatgctgg cgcacatcga acgcgggcgc gcataacgcg cccgctgcca gtcagccgtc
     9061 accccatttc agtttctcgc gtaacacttc gtagtaactg ctctctggaa atcggatcag
     9121 cctggtcagg tgctcggagg catgaatttc cacggtgtcg ccctgactca gggccacacc
     9181 gacctggcca tccagcgtcg ccattgctcc atcatccttg ctggtcaaca ccacttcaat
     9241 ttgagcgctt gccgggacga tcaaggggcg atgcgtcagc gtatggggac agatcggagt
     9301 gagaatcaat gaggggacgg ccgggttgat aatcggtccc cctgctgaga gtgaataggc
     9361 cgtcgatcca gtcggcgtgc tgatgatcaa cccgtcaccg cgcaaattcg tgacaaactg
     9421 accctgaatc gcaatcttga gttcgatcat ccgggcaagc gtgcccttgc tgatgaccac
     9481 atcattcaag acgacccctc gcgccaccgt ttcgccgtgc cgatggacat gggtcctcag
     9541 catcaacctt tcatcgagca cgaaatcatt ggcgaacacg cgctcgagag acggatacaa
     9601 attctccagc acgacttcgg tcaaaaatcc gaggccaccc atattgactc cgagaatggg
     9661 tatcccgcgt tcgccggcca ggcgcgcagc attcagcatc gtgccgtcgc cacccagaac
     9721 cagcaacaca tcggccttac tggctaattg agttttctga aacccgcctt gttctccaag
     9781 caatgttgtc gacgtcgtat ccaggagcac gtcgatgttt cgcgagcgca accaactcac
     9841 cacggcctgc accgtggcct tcacttcagg aaatttgggt ttcgtcagaa tgccgatgct
     9901 tttgcttttc atacgtaaga ccgcgtcgcg ctgaggtgag gagccggttg aaaggtgcgg
     9961 gattgtatcg ggatgattct tttactgtca aaccggcgcg cggcttggcc tcgcatctgc
    10021 gcatcgtaaa tacacgcgct tcaccagtcg cagcgcgtcg tttgcgattc gctcaatacc
    10081 ttgacacccc aaattgcggt agttagaatc acatcatcaa gtattttcga gcctttgacc
    10141 tgcatcgatt cactcagcta aggcttctct tacagacccc atcaccataa ggaggcggga
    10201 tgttcgaacg gttcacggac aaggg
//
LOCUS       DAOQEW010000139        10207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2060_length_10207_cov_8.198089, whole genome shotgun sequence.
ACCESSION   DAOQEW010000139 DAOQEW010000000
VERSION     DAOQEW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2060_length_10207_cov_8.198089"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..418
                     /locus_tag="PLS35_13885"
     CDS             200..418
                     /locus_tag="PLS35_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19808.1"
                     /translation="MIDIQYLTQAQLAVRWQVAESTLERWRSEGIGPIYLKMMGRVRY
                     RLSDILAFEAESLRKSTSESYTTCNSTR"
     gene            complement(666..1892)
                     /locus_tag="PLS35_13890"
     CDS             complement(666..1892)
                     /locus_tag="PLS35_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19809.1"
                     /translation="MHKPFGQIPWPNPVAASNVADAARHVESSLDDDPDPDVHPGSDR
                     FPRPGLDIPSADDSQEVVDEVDPASLPVSQGQDEESHEGAAGGVVEGGVEYLAFYKSF
                     RDVMRAPARKRWGIFFIKKRCFALATDMSFSTGESFADCLDALAAFLYTHELYHYRFD
                     AHCLQMEATGGLPVYRPYRRLVAKRPINEWHEESIANFYGLKAVQPSPRFPYPQPVYD
                     YLWDLVANSPGAYAGGIDKQQRSRKEHMVQQASAAFGNAGPTVWQDLVKSTIRTGMGL
                     SKPRESTLSSYLHLDNCPVYWIDWVKGGKSVLVPYAASVAEINNDFIRRYLAGVQDHH
                     SDHSYYRIDNGEKVKLPNPHRADLTNREFHNIIGKAGMTSPQFYKERDRTSVWRKGVP
                     RSPVLPPRFPSKGGAS"
     gene            complement(1938..2720)
                     /locus_tag="PLS35_13895"
     CDS             complement(1938..2720)
                     /locus_tag="PLS35_13895"
                     /inference="COORDINATES: protein motif:HMM:NF022958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCL family protein"
                     /protein_id="HNI19810.1"
                     /translation="MAKPVEIGSRSFRTQKSALDHYKEILHRYRDGERVADPSDHADL
                     VSLIERYDPILEDVGEPAKGCGQIGHFERRLNTGTGWSNSGFWVVRQDGTATDFSYIW
                     AVKGLAGDRSKDFYGACREAVALDLVLAKKGAFAEYGDDQGRVECELTGVLVTIDDAH
                     LDHAWPYFSHLVSGFRAARGWSRDIPDGVVSAPADGQTTATFIDTSVADAFRAYHHDQ
                     AILRILSRTANLQTASQARRPRVARPVRVPDRYNVPLDLGRR"
     gene            complement(2751..3722)
                     /locus_tag="PLS35_13900"
     CDS             complement(2751..3722)
                     /locus_tag="PLS35_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNI19811.1"
                     /translation="MANITVVKGNIVRKTDCDGVVNSANPNLRAGSGVCGAIYAAAGP
                     RLEPYSSQFAPLQIGEALATPAFDMDCRYIIHTRGPKYYEDADPPGSLEKAMRNTIAL
                     ADENGLTRLAVPAISMGVYGYPAEEAIPILVRVASQMAGVLQNLLEIRFVVVSDELLH
                     LFRRSIEQHVTSADECTPQALVPLRVSRVKESEVRDKLEQFHILTYAEDGFSPLDIHA
                     RRLGIDRELVKAFVSEVNDRSAVGSLHPKAPVSAVPRELVRDRQDVDALAASIGELLR
                     VNRETIKARRLAFDFRTPSVPEFTVAALNSAMRAQESGLEEILILEM"
     gene            complement(3753..4541)
                     /locus_tag="PLS35_13905"
     CDS             complement(3753..4541)
                     /locus_tag="PLS35_13905"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNI19812.1"
                     /translation="MGLRIALAVLITLFSVYAVWIEPNWLDVSTHDMRSDLAGKGIRV
                     VQLSDLHIQDFSHREVEVANRVKSLKPDLVILSGDVIDRLDRLPTLHSFLAALGDTPT
                     VAVLGNWEYWADVDLHALRDEYRHHGIRLLVNEVASYRVRLRTLHIVGMDDFTAGQPD
                     GGLLNAPLKGGTAVLVQHSPGYFEKLPTNLRSGIFNLCLSGHTHGGQITLFGLPIWRP
                     PGSGPFASGWYETTACKLYVSKGLGTSLLPVRFGARPEIAVFDL"
     gene            complement(4545..5234)
                     /locus_tag="PLS35_13910"
     CDS             complement(4545..5234)
                     /locus_tag="PLS35_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19813.1"
                     /translation="MSKQADGGIQSWLRAHPYPPFLEHLSFRLGNQLFFVRVEDVDGK
                     VRGPGNPQGFITAARMANGRACILPMKKKLFGGAWVADMPGWGLLDPDTRKPIDPVAL
                     VTDQKIEMTPWEVHDMAVQVVRDHLDKQGFELMSWQGNPEVDPSIWFIGQSKRPEWVV
                     VRSAIFPANSAERPSNWQAIAASCAKMSAIGHFACVALASVDEIFDPGAEKPMPLWRG
                     HGMHVRFTGLE"
     gene            complement(5312..5743)
                     /locus_tag="PLS35_13915"
     CDS             complement(5312..5743)
                     /locus_tag="PLS35_13915"
                     /inference="COORDINATES: protein motif:HMM:NF023496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HNI19814.1"
                     /translation="MQYKKGDRVKHPKKPDWGIGQVLADSTGGTVRIFFTHAGEKTMS
                     LDFVQPEKLSGDVASSPILDQLNLTAQPTKDAKGKVLCTNCGAPTQFGETANPKRYQL
                     GWCEPCFKHSQRTFKDETTGETRYIDEFRTIDGIKSRYGPH"
     gene            complement(5777..6424)
                     /locus_tag="PLS35_13920"
     CDS             complement(5777..6424)
                     /locus_tag="PLS35_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19815.1"
                     /translation="MIYLYWYLGIGAVVLAIVYGAHHLTKEKESDSLRELLEAVNPDR
                     KKLSYRILNNIVAPVLAAILVVAVWPVAIYMKVKEMLQKKESYVSPEEREFAVEREHL
                     LERLTAQEIERREVVTDPLKAAPDLPFGHLNAAWQEFLQGKADGAELWSFSAQWQTTW
                     GRKELRCGYAVVQDGAPGAHFLTVWKDIPEEAEGGGSAKKASAGDIPGWLRKQAD"
     gene            complement(6421..6921)
                     /locus_tag="PLS35_13925"
     CDS             complement(6421..6921)
                     /locus_tag="PLS35_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19816.1"
                     /translation="MTAQIAERLHYQGEDVAMCTNPLSDYFAMGGTNPRFESNCTALW
                     RGYVGSWEIVDDRLYLVGLSGTLEGGADATLATIFPDFPDRVFAHWYSGTIRIPQGKQ
                     LEYVHMGYGSTFERDLFLDIERGVIVATRVRHNGTAESDNAPESYEVGAMTVFPRSGK
                     GEGESA"
     gene            complement(6971..8845)
                     /locus_tag="PLS35_13930"
     CDS             complement(6971..8845)
                     /locus_tag="PLS35_13930"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNI19817.1"
                     /translation="MPNSAEMHLKPEALARYGLPDIAYPIESADLQAALTGGGELPLA
                     MMLHGLQEVARDGETDWKHLEPAMSRLSELLAPEDERDVISAAGDTWWIEIGPVDLSG
                     KLVTVQRGNDLIAAITPRDDGRLRVAVFRPLDAKSAEYLTGLGQVPHPEHGVCMRENN
                     WEYALDCSAGNGNYYAADRGEAYLSYWEKGLGISWDGTEVPEWRTQLSLVARPAAQAV
                     VDLGVHHSLSGDGDLEPDDDASDEADDDSTPLGWRSKRQQRRTVQGRFAGCLLGGAVG
                     DALGAPVEFMKRTEILRKFGPKGITQYAPAYGGLGMITDDTQMTLFTAEGLIRAWVRG
                     CMKGITTYSGVTAHAYLRWLQTQGERPTCDIDFGTDETGWLFQQRELHSRRAPGNTCL
                     AALRAMKSLGEPARNDSKGCGGVMRVAPVGLFAWRLRQQDSPHDAFRLGAELAALTHG
                     HPTGALTGGVLAVLILALTDGASLPEALVVSKAILRSEPGHEETLRAIELAETLAASG
                     QPHEAAISQLGQGWIAEEALAISIYCALVARNFKHGVILAVNHDGDSDSTGSITGNLL
                     GAMHGVRAIPAEWLEPLELREVITSLAEDLYAFKDWDIGEYSENVEMNQRIWQKYPGF
                     "
     gene            complement(8829..9344)
                     /locus_tag="PLS35_13935"
     CDS             complement(8829..9344)
                     /locus_tag="PLS35_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNI19818.1"
                     /translation="MKAVTFAADKHRNQRRKDADASPYINHPIALANVLANEGGVNDV
                     VVLCAAVLHDTIEDTETTPEELIAVFGPQVTATVLDVTDDKSLDKHVRKQRQIEHAPH
                     ISKEAKLVKLADKICNLRDILASPPADWSPERKQGYFDWAAKVVAGVRGVHPELEAVF
                     DGLLARHAELR"
     gene            9546..>10207
                     /locus_tag="PLS35_13940"
     CDS             9546..>10207
                     /locus_tag="PLS35_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNI19819.1"
                     /translation="MSNEQLADLTQPQRDRLAFVELRVRFIGEIRRQDLVSRFGIQSA
                     AASRDLALYKDMAPGNIDYDSKGKSYVLGPDFRPVFDFPPERVLSWLTQGFGDGEPMR
                     LKAWVISESPSRLTHPDLDVLASVTRAIHQECPLGIEYHSISSGRTEREIVPFALIDN
                     GLRWHVRAFDRKSQEFRDFVITRINRPAVLKGQPVAPHEMSDQDIQWTRIVELELVPH
                     PD"
BASE COUNT         2054 a         3133 c         2865 g         2155 t
ORIGIN      
        1 aaccataacg aaaaaccgct acggtgtcca gcggagatct ggcgaagggt aaggcatttt
       61 gtgggggcac tcgcggaatt cccggttccg atagtcactt cggatgcgct gccccgcaac
      121 cggtattggc cgatttacgc tatccaccat gacgaatcct gagtagctgc aatttcgcag
      181 cttcaacgga ggagtcgtca tgattgatat ccagtaccta acccaggccc aactggctgt
      241 acgctggcaa gtcgctgaga gcacactcga gcgctggcgc agtgagggca ttggtccgat
      301 ctacctgaag atgatgggtc gcgtccgcta tcgcttgtca gatattctgg cgtttgaagc
      361 ggagagcctt cgcaagagta cgtccgagtc ctatacaacc tgcaacagca ctcgctgata
      421 cgaaaaagcc ctcagttccc tcgctggctc tttcgaacac ctgcgaactc ctgagggcta
      481 tttcgggcta ttactagcca atgcgaattt ggtggtggag tatagcactg gagcggacgg
      541 tcctcccgca ggcctgcacc acagggcgaa ggcgggaggg attccgcgcg acatggagta
      601 aggattccgt tccccgcgct gttcccacca tgccccgaca tgcattcatc tagacccaaa
      661 cgcctctaac tcgctccacc cttcgatgga aaacgcggcg gcagcaccgg gctgcgaggg
      721 acaccttttc gccaaacaga cgttcgatcc ctctctttgt agaactgagg gctggtcatc
      781 ccggccttgc cgatgatgtt gtggaactcg cgattggtca gatcagcgcg gtgcggattt
      841 ggcagcttaa ccttctcccc gttgtcgatc cggtagtagc tgtgatcgct gtggtggtcc
      901 tgaacccctg ctagataccg cctgatgaaa tcgttgttga tctcagcaac ggacgccgcg
      961 tatggcacca aaacactctt tccacctttg acccagtcga tccagtaaac cgggcaattg
     1021 tccaggtgca agtaggagct cagtgtggat tcgcgtggct ttgagaggcc cattccggtg
     1081 cgaatggtcg acttgaccag atcctgccaa acggtcggac cagcgttccc aaacgctgcg
     1141 ctggcctgct gaaccatgtg ttcctttcgg gacctctgct gcttgtctat gcctccggcg
     1201 tatgcgcccg ggctgttagc caccaggtcc cacaagtagt cgtatacggg ctgagggtag
     1261 ggaaatctgg ggctcggctg aaccgctttc aggccgtaga aattggcgat cgactcctca
     1321 tgccattcgt tgatcggccg tttcgcgacc agcctacggt atggcctgta caccggtaac
     1381 ccgccggttg cttccatctg aaggcagtgg gcatcgaacc ggtagtgata cagctcgtgc
     1441 gtgtacagaa atgccgccag ggcgtctagg caatcggcga atgactcccc ggtgctgaag
     1501 ctcatgtcgg tggccaaagc aaaacagcgc ttcttgatga agaaaatgcc ccaccgtttg
     1561 cgcgccggag ccctcatgac atcccggaaa gacttgtaaa aggccaagta ctcgacgccc
     1621 ccctcgacga ctccgccggc agcgccttca tgactttcct cgtcttgtcc ctgactaacc
     1681 ggtagagacg cggggtcgac ttcatcaacc acctcttgag agtcatcggc gctcgggata
     1741 tctaatcccg gccgggggaa ccgatcggag ccagggtgga catcgggatc ggggtcgtca
     1801 tccagcgagc tttcaacatg gcgcgccgcg tcagcaacgt tggatgcagc aacggggttg
     1861 ggccagggga tttgtccaaa cggtttatgc atgtgcgacc tcgatttcag cctcttgggc
     1921 gatagaactc cgacccttca tctgcgtccg agatctaatg gtacgttgta tcgatccgga
     1981 acacggacgg gacgcgcgac tcgtggccgt cgagcctggc tggcggtctg caggttcgct
     2041 gtccgagaaa gtatccggag gattgcctgg tcgtggtggt acgcccggaa ggcatcggcc
     2101 accgaggtat cgatgaacgt ggccgtcgtc tggccatccg ccggagccga cacgacacca
     2161 tccgggatgt ctctggacca gcccctggca gcccgaaagc cactcaccaa gtgtgaaaag
     2221 tacggccaag cgtggtccag gtgtgcgtca tcgatggtga ccagcacgcc ggtcagttca
     2281 cactcaaccc gcccttggtc atccccatac tctgcaaagg cacccttctt agctaggacc
     2341 aggtcgagtg cgactgcctc acggcacgca ccatagaagt ccttcgaccg gtcccccgca
     2401 aggcctttga cggcccaaat gtacgaaaag tccgtggcag tcccatcttg gcgcaccacc
     2461 cagaagcctg agttgctcca gcccgtcccg gtattcaagc gccgctcgaa gtggccgatc
     2521 tgcccgcacc ccttcgccgg ctcaccgaca tcttcaagaa tcgggtcata ccgctcgatg
     2581 agcgacacga ggtcggcgtg atcgctcggg tcggcgacac gctccccgtc ccgataccga
     2641 tgcaggattt ccttgtagtg atcaagagcg cttttctggg tccggaagct ccgggaaccg
     2701 atttcaacag gctttgccat ccgttactcc ccacaaattg ataccaaaca tcacatttcc
     2761 aatatcagaa tttcttccaa gccagactcc tgcgcacgca tcgcagaatt caacgcagca
     2821 actgtgaact caggtaccga cggcgtacga aaatcgaagg ccaaacgtct ggctttgatc
     2881 gtttcacgat tgacacgcag gagctcccca atgcttgcag ccagcgcatc tacgtcctgc
     2941 cggtcgcgga ccagttcacg cggcacagca ctgacgggcg ccttcggatg aagcgagcca
     3001 actgcggatc gatcgttgac ttcactcaca aatgccttca caagctcgcg atcgataccg
     3061 agtcgtcgtg cgtgaatatc cagtggtgag aatccgtcct cggcataagt caaaatatga
     3121 aactgctcca gcttgtcgcg aacctctgac tccttaactc ttgaaactct caggggtacg
     3181 agcgcctgcg gggtgcattc atcggcggat gtgacatgct gttcaatcga ccgacggaac
     3241 aggtgcaaga gttcatcgct caccaccacg aaacgaattt ccagtaggtt ctgaagcacc
     3301 cctgccattt gggatgcaac acgcaccagg attggaatgg cctcctccgc tgggtagccg
     3361 tacacaccca tcgaaatcgc aggaacagct aatcgggtta agccattttc atcagccaaa
     3421 gcgatggtgt tgcgcatggc tttctcgagg ctaccgggag ggtcggcatc ctcgtaatac
     3481 ttggggcctc gggtgtggat gatgtagcga caatccatat caaaagcagg tgttgccaac
     3541 gcttcgccta tttgaagagg cgcgaactgc gagctgtagg gttcgagacg gggccctgct
     3601 gctgcgtaaa ttgcgccaca gactccacta cctgctcgca ggttcggatt tgccgaattg
     3661 acgacgccgt cgcagtcggt cttcctgacg atgttgccct tgacgacggt gatattggcc
     3721 atggtcatat ttaatccctt cttaatcgca gatcaaagat caaacacagc gatctctgga
     3781 cgcgccccaa atctgactgg tagaagtgac gtcccgagtc ccttcgagac gtagagtttg
     3841 caggccgtcg tctcatacca ccccgaagca aaaggaccgc tgcccggggg cctccatatg
     3901 ggcaacccaa aaagagtgat ctgaccacca tgggtgtgcc cggaaaggca caggttgaat
     3961 attcctgaac ggagattggt cggcaatttt tcaaaatagc ctggcgagtg ctgcactaac
     4021 accgctgtgc cgcccttaag gggtgcgttc aagagtccac cgtctggttg gccagcagtg
     4081 aaatcatcca tgcccacgat gtgaagagta cgcagccgaa cccgataaga agccacctca
     4141 ttcaccagaa gccgtatccc gtggtgtcgg tactcgtcgc gtaacgcatg aagatcaacg
     4201 tcagcccagt attcccaatt acccaaaaca gcaacggtgg gcgtatcgcc cagagccgca
     4261 agaaaggaat gaagagtcgg aagccgatcc agcctatcaa ttacatcgcc agaaaggatt
     4321 accaaatcgg gcttaaggga tttgactcgg ttggcaactt ccacctcacg gtgactgaag
     4381 tcttgaatgt gcaaatcaga gagttgaacg actcggatgc ctttccctgc gaggtccgat
     4441 cgcatgtcgt gagtactgac gtctaaccag ttcggctcga tccagacggc atacacactg
     4501 aacagagtta tcagcacagc gagcgcgatc cggaggccca tgccttactc cagcccggtg
     4561 aaacgcacat gcattccatg cccacgccac aacggcatag gtttctccgc gccggggtcg
     4621 aagatctcgt ctacgctcgc taatgcgaca caggcaaagt gaccaatcgc gctcatcttg
     4681 gcgcagctgg ctgcaatcgc ctgccagttg ctcgggcgct cggcgctatt ggccggaaat
     4741 attgcggaac gcactaccac ccactccggc cgtttggact gaccgatgaa ccagattgag
     4801 ggatccacct ccggattgcc ctgccaggac atcaactcga agccttgctt gtccagatgg
     4861 tcgcggacca cttgcacggc catgtcgtga acctcccatg gcgtcatctc gatcttctgg
     4921 tccgtgacca aagcgacggg atcgatcggc tttctggtat ccgggtccag caagccccat
     4981 cccggcatgt ctgccaccca ggcgccaccg aaaagcttct tcttcatcgg caggatgcag
     5041 gctcgaccgt tagccatcct ggcagccgtg atgaacccct gcgggtttcc ggggccccgc
     5101 actttcccat cgacgtcctc gacgcgcacg aagaacagct gattgcccag acggaacgac
     5161 agatgctcca ggaacggcgg ataggggtgt gcgcggagcc atgactggat cccgccatcg
     5221 gcctgtttgc tcaagtggat gccggctgcc ttccagcagg ggaagaatgc ttccgacatc
     5281 tcatgcattt caatttcgta catgctgttc ctcagtgcgg gccatagcgg ctcttgatac
     5341 cgtcgatcgt tcgaaattcg tctatgtagc gcgtttcccc agtggtttcg tctttgaacg
     5401 ttcgctgact gtgcttaaaa cagggctcgc accaacccaa ttgataccgc tttggatttg
     5461 ctgtttcgcc aaactgcgtc ggcgcgccgc aattcgtgca gagaactttc cctttcgcat
     5521 ccttggtggg ctgcgcggtc agattcaact ggtccaggat cggactcgac gccacatcgc
     5581 ccgacagctt ctcgggctgc acgaaatcca gtgacatcgt tttctcgccg gcatgggtga
     5641 agaagattcg taccgttccg ccggtactgt cggcaagcac ctggccaatg ccccaatccg
     5701 gcttcttcgg atgctttacg cgatcgcctt tcttgtattg catgggatct ccttgatgag
     5761 tcgtcgcgcc tgactctcag tcagcctgct tcctgagcca gcctgggatg tcgccagcgc
     5821 tcgccttttt ggcactgcca ccgccttccg cctcttccgg gatgtctttc cacaccgtca
     5881 gaaagtgggc gcccggcgct ccgtcctgga ccaccgcgta tccacaccgc agttccttgc
     5941 gtccccatgt cgtctgccac tgtgccgaga acgaccacag ctcggcacca tcggctttgc
     6001 cttggaggaa ttcctgccac gcagcattaa ggtggccaaa gggcaaatca ggtgcagcct
     6061 tgagcggatc tgtgacaacc tcacgcctct cgatttcctg cgccgtcagt cgctccagca
     6121 ggtgttcgcg ctcgactgca aattcgcgtt cctccgggga cacgtagctt tccttcttct
     6181 ggagcatctc cttgaccttc atgtagatcg ccactggcca gacggcgacc acgaggatgg
     6241 ccgcaaggac gggggcgacg atgttgttga ggatgcggta ggaaagcttc ttgcgatccg
     6301 ggttgacggc ttcgagcagc tcacgaaggg agtcagactc tttctccttt gtcaggtgat
     6361 gtgcgccgta gacgatggca aggacaaccg cgccgatgcc gaggtaccag tagagataga
     6421 tcacgccgac tccccctcac ccttaccact tcgagggaac accgtcatgg cgccgacctc
     6481 gtagctttcg ggagcgttat cagactcggc ggtaccgtta tgacgaaccc gggtggccac
     6541 aatcacacct cgctcgatat ccaagaacaa gtcccgctcg aaggtgctgc cgtagcccat
     6601 gtggacatac tcgagctgtt tgccctgggg gatgcggatc gtgccggagt accagtgggc
     6661 aaacacgcga tcgggaaagt ccgggaagat ggttgccaaa gttgcatccg ctccgccctc
     6721 tagcgttccg ctcagcccca ccaggtacag gcggtcatcc acgatttccc agctaccaac
     6781 gtagccacgc cacaaggccg tgcagttgga ctcgaagcgc gggttcgtgc cccccatggc
     6841 gaaatagtcg ctgagggggt tcgtgcacat ggcaacgtcc tctccctggt agtgcagtcg
     6901 ttctgcaatc tgagcggtca tgtcattact cccatgcgag gtgtttgttt ccagtggcca
     6961 ccgttttcat ttagaacccg gggtatttct gccaaatgcg ctggttcatc tctacgttct
     7021 cgctgtattc gcctatgtcc caatctttga aggcgtacag gtcttccgca agggacgtga
     7081 tgacttcgcg caactccaga ggctccagcc attcggctgg tatcgctcgg acgccgtgca
     7141 tggcgcccag caagttgccg gtgattgagc ctgtcgagtc agagtcaccg tcgtggttca
     7201 cggcgaggat gacgccgtgt ttgaaatttc gtgccacgag cgcacagtag attgagatgg
     7261 ccagcgcctc ttcggcgatc cacccctgac caagctgtga gatcgccgct tcatgaggtt
     7321 gaccactcgc agccagcgtc tcggccaact cgatcgcacg cagcgtttcc tcatgtccag
     7381 gctcggatcg gaggatggct ttcgatacga ccagcgcttc gggcaatgag gcgccgtcgg
     7441 ttagcgcaag gatcagaact gccagcacac cgcctgtcag cgcaccggtc ggatgtccgt
     7501 gcgtcagggc tgccaactcg gcgcccaagc ggaaagcgtc gtgcggtgag tcctgctggc
     7561 gcaggcgcca agcgaataac cctacgggtg cgacccgcat gacgcccccg caccctttgc
     7621 tgtcgtttcg ggcgggttcc ccgagcgact tcatcgcccg cagcgccgcg aggcaggtgt
     7681 tgccaggggc acgtcggctg tggagttccc gttgctgaaa gagccagcca gtttcgtcgg
     7741 tgccgaagtc aatgtcgcac gtcggacgtt cgccttgggt ctgaagccag cgcaggtaag
     7801 catgagccgt cactccggaa taggtggtga tgcccttcat gcagccgcgc acccacgccc
     7861 gtatcagccc ctcggctgta aacagggtca tttgagtgtc gtcggtaatc atgccgaggc
     7921 cgccgtaggc tggcgcgtac tgggtgattc ccttgggacc gaatttgcga aggatctcgg
     7981 tccgcttcat gaactcaact ggtgccccaa gcgcgtctcc gacggcgcca cccaagagac
     8041 agccagcaaa tcggccctga actgttcggc gctgctgacg cttgctgcgc cagcccagtg
     8101 gcgtggagtc gtcatcggcc tcatcgctcg catcatcatc gggctccaga tcgccatccc
     8161 cggacaagga gtggtggacg ccaagatcga caaccgcttg cgcagccgga cgggcaacga
     8221 ggctcaactg cgtgcgccac tcgggaactt cagtaccgtc ccaactgatg cccaggcctt
     8281 tctcccagta ggacaggtag gcctcgcccc ggtcggctgc atagtagttg ccatttcccg
     8341 cagagcagtc gagcgcgtac tcccagttgt tctcgcgcat gcaaaccccg tgctccgggt
     8401 gcggcacctg gcccagacca gtcaggtact ccgcactttt ggcatccagc gggcggaata
     8461 ccgcgaccct tagccggccg tcgtcccgtg gtgtgatcgc ggcaatcaag tcgttaccac
     8521 gctgcacggt aaccagcttg ccgctgaggt cgactggccc gatctcgatc caccaggtgt
     8581 cgccggcggc cgagatcaca tcgcgttcgt cttccggcgc cagcaactcg gacaagcgac
     8641 tcattgctgg ctcgaggtgc ttccagtcag tttccccgtc gcgcgcaacc tcctgaaggc
     8701 catgcagcat catggccagc ggcagttccc cgccccctgt cagtgcggcc tgcagatcag
     8761 cgctttcgat cggataggcg atgtccggca ggccgtagcg cgccagcgcc tccggcttga
     8821 gatgcatttc agcggagttc ggcatggcgg gccaagagtc cgtcaaacac ggcttcgagt
     8881 tcaggatgga cgccgcgaac acccgccacg actttggctg cccagtcgaa atagccttgc
     8941 ttgcgctcgg gcgaccagtc tgccggcggt gacgccaaga tgtcccgcag gttgcaaatc
     9001 ttgtcggcca gcttcacgag cttggcttct tttgagatgt gcggtgcgtg ctcaatctga
     9061 cgctgcttgc ggacatgctt gtccaaagac ttgtcgtcgg tcacgtccag cactgttgcg
     9121 gtgacctgcg gcccgaaaac cgctatgagc tcttcgggtg tggtctcggt gtcctcgatc
     9181 gtgtcgtgca ggaccgctgc gcacaaaacc accacatcgt tgacgccccc ttcgttcgcc
     9241 agcacgttgg ccaaggcaat tggatggttg atgtagggcg acgcatcggc atccttgcgg
     9301 cgctgattcc ggtgcttgtc tgcagcgaag gtcacagcct tcacaaacaa gcctagtggg
     9361 tctgagtgct ggttcattta ccggtccctt tcctagatct atggcgcctt accgaacgat
     9421 tgtgattatc attttacgac acaccaccat tcgatcatag tttattggac tcattctaga
     9481 gcacaggata tactttccgc aacagccctc accagccatc gctaacccga atgagcaccc
     9541 acaggatgtc gaacgaacag cttgcagatc taacccagcc acagcgcgac cgcctcgcat
     9601 tcgtggagtt gcgcgtgcgc tttatcgggg agatccgtcg ccaagacctg gtttcgcggt
     9661 tcggaatcca gtcagcggcg gccagtcggg atctggcgct ctacaaagat atggcgcccg
     9721 gcaacattga ctacgactcc aagggcaagt cgtacgtcct gggcccggac ttccgtccgg
     9781 tctttgactt cccgcctgag cgcgtcctgt cttggctgac ccaaggtttc ggcgacgggg
     9841 agccgatgcg gctaaaggca tgggtgatca gcgagagccc gtcacggctc acgcatcctg
     9901 atctggatgt gctggctagc gtgactcggg cgattcacca ggagtgtccg ctcggcatcg
     9961 agtaccactc catctccagt ggccgcaccg agcgggagat cgtcccgttc gcgctgatcg
    10021 acaacggtct gcgctggcac gtccgcgcct tcgaccgaaa gtcgcaggaa ttccgggatt
    10081 tcgtcatcac ccggatcaat cgtccagcgg tgctcaaggg ccagcctgtg gcgccccacg
    10141 agatgagcga tcaagacatt cagtggaccc ggatcgtcga gctggagctg gtgccgcacc
    10201 cggatca
//
LOCUS       DAOQEW010000140        10180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2075_length_10180_cov_6.405432, whole genome shotgun sequence.
ACCESSION   DAOQEW010000140 DAOQEW010000000
VERSION     DAOQEW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2075_length_10180_cov_6.405432"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2077)
                     /locus_tag="PLS35_13945"
     CDS             complement(<1..2077)
                     /locus_tag="PLS35_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HNI19820.1"
                     /translation="MSTILEWKVKQLVDRTAISDWLPYEAWDDTNQVYRLDDGRIGVV
                     FSSKPLLGLSEESIQKLTNLFESDLPIGTTFQFMLHASPIIEPYLDNHVILAQRGQAG
                     ERYVADARKTAEFYLHARDTQLTSNPPLRPRDFTVYVSVAFPTQETTAQGWEDERIHQ
                     AVSGIEGQLNTLGLLPERVGPLQLLSVLHVLLNPGHSWESRPPAYSDQLPIRDQAVRL
                     DTKATLRTKMIELDGKFLKTLTVQSWPVQWHGGNNRELIGSLIRFTDQITCPFFLTLN
                     VLKFDQVKAKGKLRKKHVIINQQASGFLLGLIPSLKFKLDHFNGVQAQLENGRQLIGS
                     YFQVVLYGDTPRHVEEQTGALRALYRAKDINLQEDHFIGWKILMTGLPLGLPSDDKAL
                     IDGLRRMKTYHTEVPAHICPIVADWKGGGAPVVLLTSRSGQLITMDVFANQQTNYNMA
                     IAATSGAGKSFFMNNWIKSYLGIGGQVWVIDAGFSYVKLVELLKGQYIQYGANAQRLC
                     FNPFSKLVHWGSNAGGVDDPSDRADELATLKALHAQMASPSRPLSDVELSFIEEAIMR
                     VLEGEGCEGCPDSVHKVLKSMTDPRARDLARVLASYAKGGMYSHLFNGANNVNFENDF
                     VVLELDGLNEQKQLRSVILLQMQMNIQAVMYKKENRGRRKFVILDEAWDLLSQGGNTA
                     AFFETGARRV"
     gene            complement(2102..2833)
                     /locus_tag="PLS35_13950"
     CDS             complement(2102..2833)
                     /locus_tag="PLS35_13950"
                     /inference="COORDINATES: protein motif:HMM:NF021212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HNI19821.1"
                     /translation="MMSGYIRRYITRGGSWYGLGSGVAMGLSVLLAGCGGYESNFSCK
                     GYPDQATCESVSEAYEKRFSAGQKVTQYTKDQKGVGQDTSSSSGPGQVTPPMIFSGKP
                     DSAIGKPVITPVSALRVTVFPWKDTKKRLHDQSRHYLIVDEPDFIFGHMAQGVSAGSS
                     SGHGKDLYPRMGRMVEKRDGKRTPSGNAAANAMTENVPEVGQRSAPMSVNPLGSGGIM
                     PQGFPQLPQTGASNYLTDDGPLPPQ"
     gene            complement(2872..3549)
                     /locus_tag="PLS35_13955"
     CDS             complement(2872..3549)
                     /locus_tag="PLS35_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNI19822.1"
                     /translation="MRPIKTVIEQKFPGMKVLRVSKTEIPGWLLVESDSDRVENYMYV
                     HESGRYVLSGALFDIQMGRNVTQEYVRDRQQALLAGMDTSKTILLSPMQSKLERPVLV
                     FDDPDCRFCQQFHPEVQKLVEAGVPVAVVLYPLVRTHPDAYRKSVAIWCAPDQAEALG
                     RALMSKPVMGEVGACSHPIDENIKLGKRLGVNSTPMVFLPNGQSFAGYRPASEVLALL
                     QIEAQQK"
     gene            complement(3678..4964)
                     /locus_tag="PLS35_13960"
     CDS             complement(3678..4964)
                     /locus_tag="PLS35_13960"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HNI19823.1"
                     /translation="MASIKPTESAILNPGDPIRPEKPAPSKLLAGVVLVGGFIVLSMV
                     LQQFRGNQPGIVRGKSPAKTVSINREETDKEIYMKDAGARIEALEQQRDQMRRLLEQD
                     GRDKTEIKTQLSDLQKRIESVARSGGVVSAGTKSGALPPLPPPLSANPVGGGSATPVS
                     AMPVPKGPIPVQSTPKPAGGTLPAPGAGGGLTEGFQVFRPEIVPGAKKNEKKLWLNTG
                     TIIPVKLLSGMDAPARSTGAQGGGASANSPYPVLMMVRDIMKLPNEARIDAKDCFLVG
                     EGLGDLSSERVMIRGNALSCVKKNGEAVDIELKGFVSGEDGKQGMRGRVVLKEGALLG
                     RTLLAGFVSGISRAFMPYQSGFFVAQSPSQAFNLPPAGPVAMSGMAGGAGRAAELLAR
                     HYSMMAASIFPVIEIDAQREVDFIVGTGRELEGFFQ"
     gene            complement(4985..5758)
                     /locus_tag="PLS35_13965"
     CDS             complement(4985..5758)
                     /locus_tag="PLS35_13965"
                     /inference="COORDINATES: protein motif:HMM:NF018311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HNI19824.1"
                     /translation="MTSSVYRDSKHRAMAMCVLGLLLFLSWARSVGAAVVVGDEGATS
                     LTLSGRDLNVLTFPAPITMATSSRADLKAKPEGRNLIVQVKTGPADLVVMAGEQTYVF
                     EVGVSVSLGAQTISIEDRRVGTVVYDTDPARQAADYVDGLLETLQMAARGALPKGYRQ
                     TSIAEERYPKWLELKVVQGIEYRGPKYRVIVYRLVNTAGHKYTLREQEFLTGVQKAIA
                     LNREVVESGEEVVVYLLDDAPPEPPKPKPEPKVDPDAKS"
     gene            complement(5769..6350)
                     /locus_tag="PLS35_13970"
     CDS             complement(5769..6350)
                     /locus_tag="PLS35_13970"
                     /inference="COORDINATES: protein motif:HMM:NF017152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HNI19825.1"
                     /translation="MTIELWKSKLHNAQVLNLVFAALATVLAVALVANGFFRQNVAVH
                     VVPINPTQEYWANAMGGDEYYKKSIAMAMLPWIANVTPASVEFHHRQLLQWVPSSHYG
                     LMSESLGAEAVYVKTNNLSRVFFVTASRVDGDVAVFEGIERRFVGKSEIREEEREYRI
                     SMRWDQGRPWVVGLSAAHLDRSRQDSQSDPAKG"
     gene            complement(6356..6637)
                     /gene="traL"
                     /locus_tag="PLS35_13975"
     CDS             complement(6356..6637)
                     /gene="traL"
                     /locus_tag="PLS35_13975"
                     /inference="COORDINATES: protein motif:HMM:NF018836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HNI19826.1"
                     /translation="MNGTFIPRYLDSLPQIFWWEIDELAVLFVCIFLGIVTKFLTYLI
                     VVGIVSTLILSKMKNGKSEGFLFHWAYWSGVPTFQLTGSPHGTTREMME"
     gene            complement(6716..7036)
                     /locus_tag="PLS35_13980"
     CDS             complement(6716..7036)
                     /locus_tag="PLS35_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19827.1"
                     /translation="MGNVLKRVTGEMWRMSLLLGLMVLASFVVLAVPAFAGADATFGP
                     LVTLLTNYMQGSLGTLLALVAGAIGLGSAMAGRWAGMFTGFGVAAAAFYIPNIIPTIT
                     TGLM"
     gene            complement(8371..9159)
                     /locus_tag="PLS35_13985"
     CDS             complement(8371..9159)
                     /locus_tag="PLS35_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19828.1"
                     /translation="MPSSAVGGLMLEYAADWQESLVQRAEQCARSITYVAGELIRERF
                     RLGGIVVEALERSQHGDAVVVKMAERMSAAVGKCIRPHMLYESARIYNAFGGQWERIE
                     ALRAQLSFPLSYSYLVRFCLPALTMDSAWNAEQVTYSNDSELRGFESAVLRIEARVQA
                     HRTRQQEGGKERSTDLEAEGFVGACTQSAEFQEMAVRTIVRRLDVLCEALEGKAQQRG
                     VELEALRESQEAIARIASRMATLGTLIHAPVMHAVIPDNNTSVL"
     gene            complement(9149..9961)
                     /locus_tag="PLS35_13990"
     CDS             complement(9149..9961)
                     /locus_tag="PLS35_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19829.1"
                     /translation="MRGGRKWLLAVAGALGLVCLGGNVVMALSPVNISPGYPTSLDDA
                     YPVPTDQVVIQSAIRPDITRNDSGSESGRFRATHDIRYGATENMELTVSGTSLRGPLN
                     PGTMDDPRSIQLGILARFRKQAEERELLPSISVRVLGEVPYAGDRTNPGLRGALVTSW
                     NLGNRWWLHGNLGYEVVPSFQPGHWVPHRSSIILVNLGFVKALDESSAIVGGLRFQED
                     PLNSKKQVASPELGYVLALDKHWLFSLSASRDFLGSVGQAAFRGSVGVIYAF"
BASE COUNT         2279 a         3277 c         2442 g         2182 t
ORIGIN      
        1 ggacccggcg cgcgccggtt tcaaaaaacg cagcggtatt gcctccctga gacaacaaat
       61 cccacgcctc atccagaatg acgaatttcc gccggccacg gttctccttt ttgtacatca
      121 ctgcctggat attcatctgc atctgcagga gaatgactga ccgtaactgc ttctgctcat
      181 tcaatccgtc gagttcaagc accacaaagt cattttcaaa attgacgttg ttcgccccgt
      241 tgaagagatg ggaatacata ccccccttcg cataggaagc cagcacacgc gcgagatctc
      301 tggctcgagg atcagtcatg ctcttcaaca ccttgtgtac cgaatccgga catccctcac
      361 atccctctcc ttcgagtacg cgcatgatcg cctcttcgat gaacgagagc tcgacatcgg
      421 agagcgggcg cgaaggtgac gccatttgcg cgtgcaacgc tttgagggtc gccagttcat
      481 cggctcggtc acttggatcg tcaaccccgc cggcattact cccccaatgc acaagtttgc
      541 tgaacggatt aaaacaaagg cgttgagcgt ttgcgccgta ctggatgtat tgccccttga
      601 gcaactccac aagtttgacg tagctgaagc cagcatcgat gacccacacc tggccaccaa
      661 tacccaagta gctcttgatc caattgttca taaagaacga tttacccgcc cccgatgtcg
      721 ctgcgatcgc catgttgtaa ttcgtctgtt gattggcgaa cacatccatg gtgatcaatt
      781 gacctgaacg agacgtcagc agcaccactg gagctccgcc ccccttccag tcagccacaa
      841 tggggcaaat atgcgccggg acctcggtat ggtaagtctt cattcgtcgc agaccatcaa
      901 tgagcgcttt gtcgtccgac ggtagtccga ggggtagacc cgtcataagg attttccatc
      961 caatgaagtg atcttcctgc aagttgatat ccttcgcccg atagagagcc cgaagggcgc
     1021 ctgtctgctc ctccacatgt cgcggtgtat ccccatacag aacgacctgg aaataggacc
     1081 ctatgagctg tctgccattt tcaagttgcg cttggacgcc attgaaatga tccaatttga
     1141 acttgaggga gggaataagc cccagaagaa acccactggc ctgctgatta ataatgacgt
     1201 gcttctttcg taacttgcct ttcgctttga cctggtcaaa tttcaacaca ttcagcgtca
     1261 gaaaaaatgg acaggtaatt tgatcggtga accggatcaa gctcccaatc aactcgcgat
     1321 tgttcccgcc atgccactgc acaggccatg actgaacagt cagcgtcttg agaaactttc
     1381 cgtccagttc aatcatcttg gtgcgaagcg tcgccttcgt atcgagacgg accgcctgat
     1441 ctcgtatcgg cagttggtcg ctgtacgccg gcggccgact ctcccagctg tggcccgggt
     1501 tgagcaacac atgcaagaca ctcaataatt gcagcggccc tacacgctcg ggcaataatc
     1561 ccaacgtgtt caattgcccc tcaatgcccg acaccgcttg atgaatccgc tcgtcttccc
     1621 atccttgcgc ggtcgtttcc tgcgttggga acgccacgga tacatacacc gtgaaatctc
     1681 gtgggcgcag aggtgggtta gacgtcagtt gtgtatctct ggcatgcaga tagaactctg
     1741 cggtctttcg cgcatcagct acataacgtt cccctgcctg ccctcgttgc gccagaatca
     1801 cgtgattgtc cagatatggc tcgataatgg gggacgcatg aagcatgaat tgaaacgtgg
     1861 ttccaattgg caggtctgac tcgaagagat tcgtcagctt ctgaatagac tcttccgaca
     1921 accccagtag gggcttgcta gaaaacacca caccgatacg cccatcatcc aatcgataga
     1981 cctgattggt gtcatcccaa gcctcatagg gcagccagtc actgatggct gtccgatcga
     2041 ccagctgctt taccttccac tcaaggatcg ttgacatatt cgctcctctg ggttccttcc
     2101 actattgggg tggcagcggc ccatcatcgg tcagataatt ggacgccccg gtctgaggga
     2161 gctgaggaaa tccctgcggc atgatgccac ccgagccaag agggttcacg gacatcggcg
     2221 ccgaacgttg tccaacttca ggaacattct ccgtcatggc gttcgccgcc gcattcccac
     2281 tgggtgttct ctttccgtct cgtttctcga ccatccgccc cattctcgga tacagatcct
     2341 ttccatgccc cgaactcgac ccagcactca ccccctgcgc catgtgtccg aaaataaaat
     2401 ctggttcgtc gacaatgaga tagtgccgcg actgatcatg gaggcgcttt ttcgtatcct
     2461 tccatggaaa taccgtcaca cgaagagccg acaccggcgt aatcaccggc ttgcctatgg
     2521 cgctgtccgg cttgccgctg aagatcatcg gaggagtgac ctgaccaggg ccagagcttg
     2581 agctggtgtc ttgtccaacg cccttttggt ctttcgtgta ctgcgtcacc ttctgcccag
     2641 cagagaaccg cttctcgtag gcttccgaga cggactcaca cgtcgcttga tccggatacc
     2701 ccttacaact gaaattagac tcatagcccc cacagcccgc cagaaggaca gacaggccca
     2761 tagctacccc agatcccaag ccgtaccatg atccgcctcg tgtgatgtag cgcctgatat
     2821 acccagacat catgtcgcat gcctcctcca gaatccgccc actcgacttc tctacttctg
     2881 ctgggcctct atctgcagta aagccaatac ttcactggca ggacgatagc cggcaaacga
     2941 ttgtccattt ggcaaaaaca ccattggggt ggagttcact cccaaacgct tccccaattt
     3001 gatgttctcg tcgatcggat gactgcaagc ccctacctca cccattactg gttttgacat
     3061 cagcgcgcgc cccagcgcct cggcttgatc gggagcacac caaatggcga cagacttgcg
     3121 ataggcatcc ggatgcgtcc gtaccagtgg atacagaacc acggccaccg gaacacctgc
     3181 ctcaacaagc ttctgcacct ccggatgaaa ctgctgacag aaccgacagt ctggatcgtc
     3241 aaagaccagc accggacgct ccaacttcga ttgcatggga gacaacagaa tggttttcga
     3301 ggtatccatt cctgccaaga gcgcctgctg acggtctctc acatactctt gcgtaacatt
     3361 tcggcccatt tgaatatcga agagtgcccc ggagagcacg taccgcccag actcatgcac
     3421 gtacatatag ttttccaccc ggtcactgtc gctctccacc aagagccatc caggaatttc
     3481 cgtcttggac actcgcaaca ctttcatgcc aggaaacttt tgctcgatca ccgttttgat
     3541 aggcctcacg gcatcatccg tttcggctct caggatcgac tctcccgcaa ccgacacaca
     3601 cacggcacac accagccccg cgattatctt ttcataggat cgtctcatcg ccatcatgcc
     3661 ccctttcaca ctacgaatta ctggaagaac ccctctaact cacgtcctgt cccaacgatg
     3721 aaatccactt cccgttgcgc gtcgatttca atcaccggga aaatggaggc agccatcatg
     3781 ctgtagtggc gagccaacaa ttcagccgcc cgaccggcac caccggccat tcccgacatc
     3841 gccacaggcc cggccggcgg taagttgaag gcttggctcg ggctttgtgc gacgaaaaat
     3901 ccggactggt agggcataaa ggctcgggag atgcctgaca caaaaccagc gagcagcgtg
     3961 cgacccaaca atgccccctc tttcaagaca acccgcccac gcattccctg tttgccatct
     4021 tccccactca caaacccttt caattcgata tctaccgctt ccccattctt cttcacgcac
     4081 gacaaggcat tgccacgaat catcacgcgt tccgaggaca aatctcccaa gccttctccc
     4141 actaggaaac agtccttcgc atcaatcctc gcctcattcg ggagcttcat gatgtcacgc
     4201 accatcatca gcaccgggta gggactgttt gcggaggcgc ccccaccctg cgcccccgtc
     4261 gaacgggccg gtgcatccat gccagaaagc aatttgacgg gaataatcgt ccccgtattc
     4321 agccacaatt tcttctcgtt ctttttggcg ccaggcacta tctctggccg aaatacctga
     4381 aacccctcgg tcaacccgcc cccggcccca ggggccggta gcgtcccgcc tgccggtttc
     4441 ggggtgcttt gcaccggaat ggggcctttc ggaaccggca tggccgagac aggcgtcgca
     4501 cttcctccac ccacgggatt ggcactgagc ggaggcggca gcggcggcaa cgcgccactc
     4561 ttcgtcccgg ctgagaccac gccaccagac cgcgccactg actcaatccg cttctgcagg
     4621 tcactcaact gagtcttaat ctcggtcttg tctcgtccgt cctgctccaa caatctccgc
     4681 atttgatccc gctgctgctc caacgcttcg atcctggccc cggcgtcttt catataaatt
     4741 tctttgtcgg tttcctcgcg attgatcgac acggtcttgg ctggagactt cccacggaca
     4801 atccccggtt gattccctcg gaattgctga agcaccatgc tcagcacgat gaacccaccc
     4861 accagaacca ctcccgcgag cagtttggat ggagcgggtt tctctgggcg aatcgggtcg
     4921 ccagggttga gaatcgcgct ctccgtcggt ttaatcgatg ccatcctagg tctccggtct
     4981 gaggttagct ttttgcgtct ggatccactt tcggctcggg cttgggtttg ggcggttcag
     5041 gcggtgcgtc atccagcagg tacaccacca cttcttcccc tgactcgacc acctcgcgat
     5101 ttaaggcgat tgctttttga accccggtca ggaattcttg ttcccgcagt gtgtatttgt
     5161 gtcccgcggt attcaccaat cgatacacaa tcacccggta cttcggccct cgatactcga
     5221 ttccctgcac caccttcagc tcaagccact tcggataccg ttcttcggca atcgacgtct
     5281 gccgataccc tttcggtaac gctccccgcg cagccatctg caatgtctcg agtagcccat
     5341 ccacatagtc agcggcttgg cgagcaggat ccgtgtcata gaccacagtc cccacgcgcc
     5401 tatcttcaat actaatggtc tgcgcgccta acgacacgct caccccgacc tcaaagacgt
     5461 aggtttgctc gccggccatc acgaccagat cagcggggcc agtcttcacc tggacaatca
     5521 agttgcgccc ttccggcttc gctttgagat ccgccctaga gctcgtcgcc atggtaatcg
     5581 gggcaggaaa ggtcaggacg ttcaaatctc ttccagacaa tgtgagggac gtcgcaccct
     5641 catcaccaac caccaccgca gctcctaccg acctggccca agacagaaac aacagcaggc
     5701 caaggacgca catcgccatc gcccggtgct tcgagtctcg atacacgctg gatgtcatac
     5761 gtctccccct acccctttgc cggatctgat tgactatcct gccgtgaacg atctaaatga
     5821 gccgcagaga gcccaaccac ccacggcctg ccctgatccc accgcatcga gatacggtac
     5881 tcccgctcct cttcccgtat ctcgctcttt cccacaaatc ggcgctcaat gccctcgaac
     5941 acggccacgt ccccgtccac acgactagcc gtcacaaaaa acacacgact gagattgttg
     6001 gtcttcacgt acacggcctc cgccccgaga gactctgaca taagcccata gtggcttgac
     6061 ggtacccact gcagaagctg ccgatggtga aactcaacag aggccggcgt gacattggca
     6121 atccagggca acatggccat ggcaatggac tttttgtaat actcgtcgcc gcccattgcg
     6181 ttcgcccaat attcctgcgt cggattgatc ggcaccacat gaaccgcgac gttctgccga
     6241 aaaaatccgt tcgccaccaa cgccaccgct aatactgttg ccagggcggc gaacaccaga
     6301 ttcaagacct gggcgttatg caattttgat ttccacagct cgattgtcat cgtcgttact
     6361 ccatcatctc tcgggttgtt ccgtgaggtg atcccgtcag ctgaaacgtg ggcacaccag
     6421 accaataggc ccaatggaag aggaatccct cggatttccc gttcttcatt ttcgacaaaa
     6481 tcaacgtgct cacgatcccc accactatca ggtaggtcag gaacttcgtg acaatgccga
     6541 gaaagatgca cacaaagagc acggccaact catcaatctc ccaccaaaag atctggggca
     6601 ggctatcgag atatcgtgga ataaatgtgc cgttcatctg tccctcttct gcttcattgt
     6661 gacggcgggg ccatgatctg tcccgccgtc acgacaccat gcctgtaccg cccgcttaca
     6721 tcagtcccgt ggtaatggtc ggaatgatgt tggggatata gaaggctgct gccgccacac
     6781 cgaatccggt gaacatgccg gcccaccggc ccgccatcgc cgatcccaac ccgatcgccc
     6841 ccgccaccag ggctaacaac gtccccagcg acccctgcat atagttggtc aacaacgtga
     6901 ccagcggacc aaacgtcgca tccgcacctg cgaacgcggg cacggccagc accacaaacg
     6961 aggcaagcac catgagcccc aacaagagac tcattcgcca catttctccg gtcactctct
     7021 tcaagacgtt gcccatactc cagcccctcc tctggctcgt cgctcactgt ttacaagacg
     7081 tgtggttgcc aaaccgaatg agcgccctta gttcattcca gcatagccat gctcctcctc
     7141 agcctctcct tatggtttta ctgagtcgcc tgtgacacca gtggacacaa cagatgttac
     7201 tggaggtatt tgggatcgct cctcaaccga tgagggatct ccacacctac ctcgcagcag
     7261 catcgtgatc tccacgcgtc gactgtccgc atggttcggc ataaagcagc agcctccgcg
     7321 gccgaccact tccacctgag tcgagacctt caagccagcc tgcaatgctt tcgccaccgc
     7381 gtgtgcccga gccgtcccaa gttcttcgtt tgtctcccga gaccccgtcg gatcggtgta
     7441 tccctccacc cgcagataga agacctcctc tgtggacagt tccgttaagt ctcgttgcgc
     7501 acgctccggt acagcagccg aacccatggc aaacaaaacg gttcgatgcc gcacctcagg
     7561 atcgcgacga caggcggacg gggacgtcct tcctgtgaaa ccaggaggtg ctggtttgtc
     7621 cacgctgctc accgcagcgc gagacatgtc acctggacgg cctgccagcg cacccgcaga
     7681 aaacgtcggc aatgtctgga gccgatgcat caccgcctta atcggtttcg gtgtgagatc
     7741 cgccaccagt ggtttgtttg ggacctgcgt caggggtgtt ggctctgggc atccagcggg
     7801 acgagacaga agccattgcg gaccagattg gacggaaacc accgtagtcg ggaaggtgta
     7861 gccttgcccg cagagtcgcg ttgcctcgga cattgttggc gacgcagcag gtcgcgtcgt
     7921 acagcccccg acggtcagca gtcccataca aacgagaccg gcccatggca tgtaagtcgt
     7981 ccgtccccct gtcatactcc cccctttcac cacggcttcc gagtcgccag gttcatcttt
     8041 ggtattaccg actgccacgg tgtatcgaac agaaacaggg gttgccggat gtcggaaggg
     8101 acgtcgtcga cgcgacgacg gaaccaagaa aagacgccag cgggttaaga agcgatcaga
     8161 ttaacagtag ccaccccccc ccaccccagg ccaggaagga cccgtcggga cattgctgga
     8221 ataaacgcgc gcactgaaga cactgatgaa ggtacctatc catgaattcg tcgccatcgt
     8281 gtgaacgatg cccaaacaac tctccgttac aacgaggaca cggtttcaac aaatttacat
     8341 acagtcccat tgtttgctct cctcgcttca ctacaagaca ctcgtattat tatctgggat
     8401 aactgcatgc atcaccggtg catggatcag cgtgccgagg gtcgccattc tggaggcaat
     8461 cctcgcgatt gcctcctgcg actcacgcag cgcctccaat tcgacgcctc gctgctgagc
     8521 ttttccttcc agtgcctcgc acaacacatc aagccgccgc acaatggttc tgacggccat
     8581 ctcttgaaat tctgcggatt gcgtacaggc ccccacgaac ccttcggctt cgagatccgt
     8641 cgatctttcc ttgccacctt cctgctgccg cgtacgatga gcctgcacac gcgcctcgat
     8701 acgcaagacc gcactctcga agccccgtaa ttcagagtcg ttggaatacg tcacctgttc
     8761 ggcattccag gcactatcca tagtcaaggc cggcagacag aatcggacga gataactata
     8821 ggaaagggga aagctcaatt gggcgcgcag ggcttcaatc cgctcccact gccctccgaa
     8881 ggcgttgtag atcctcgcgg actcgtacag catgtgtgga cgaatacact tcccgaccgc
     8941 tgcggacata cgttctgcca tcttcacgac gacagcatcc ccatgctggc tccgttccag
     9001 ggcttcaacc actataccgc caagccgaaa acgttcgcgg atcaactctc ccgccacata
     9061 cgtgatggac ctcgcacact gttcagcccg ttgcaccaac gattcctgcc aatcagccgc
     9121 gtactccagc atcagccctc ctactgcgct agaaggcata gatcactccc actgacccac
     9181 gaaacgccgc ttgcccaaca gatccgagga aatcacggga ggcactcaac gagaacaacc
     9241 agtgtttgtc caacgccagg acatacccca gctccggact agcgacttgc ttcttggaat
     9301 tgagggggtc ttcttggaat cgcaagccac ccacaatcgc agaagattca tccagggctt
     9361 ttacaaaccc caagttcaca agaatgatgc tcgaacgatg cgggacccaa tggcctggct
     9421 gaaatgacgg cacgacctca tatcccagat tgccatggag ccaccaccga ttcccaagat
     9481 tccaggacgt gaccaacgcc ccgcgcaagc caggattcgt tcggtctccc gcatagggca
     9541 cctcacccaa tacgcgcacg ctaatcgatg gcagcagctc tcgttcttcg gcctgtttcc
     9601 ggaaccgcgc taaaattccc aactggatcg acctaggatc gtccatcgtc ccaggattga
     9661 gcggacctcg gagcgacgtc ccactgaccg tcaattccat attttctgtg gcgccatagc
     9721 gaatatcgtg agtcgcccgg aagcgcccac tctcagagcc cgaatcattc cgcgtgatgt
     9781 cgggccggat cgccgactgg atcaccactt gatctgtggg caccgggtag gcgtcatcga
     9841 gagacgtagg gtagcccgga ctgatattta ccggggagag tgccatcacc acgttgcccc
     9901 ccaggcatac gagccccagg gcccccgcaa ccgccagtaa ccacttcctg cctccacgca
     9961 ttagtcccaa gctccttata ggaattgcac aacatagaag gttgttaccc tcgtattacc
    10021 gaaggaccaa ggacaggaaa ggcaggagat tttggatcga gtagacgtca ggggcagagg
    10081 agagaagatg tcagtgtcac gccccctgac aggtccctaa tggcccattc cagggaccat
    10141 gagccaggca caattcgcaa ggagagccgt ggatcagcgc
//
LOCUS       DAOQEW010000141         9970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2142_length_9970_cov_7.409481, whole genome shotgun sequence.
ACCESSION   DAOQEW010000141 DAOQEW010000000
VERSION     DAOQEW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_9970_cov_7.409481"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLS35_13995"
     CDS             complement(<1..271)
                     /locus_tag="PLS35_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19830.1"
                     /translation="MLPRYASVRFSSTIALLLVTLLLAMAPFAAALEIHHELAAADHD
                     GHEHSDTDLCQWVQYHTGHSLIGDVPVPCAFLAHAPPQLMPAPVLV"
     gene            452..1510
                     /locus_tag="PLS35_14000"
     CDS             452..1510
                     /locus_tag="PLS35_14000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNI19831.1"
                     /translation="MNRPIDNQHVIEIKPLPSPRDVKTQLPISDEVSELVFQTRQAIR
                     NILHGRDMDRLIVVVGPCSIHDPDAAYEYADRLKPVAEAVRDKLLIVMRTYFEKPRTT
                     VGWKGLINDPHLDGTCDIAQGIQLARTILLNINGKGLPCATELLDPVTPQYIADLLSW
                     TAIGARTTESQIHREMASGLSMPVGFKNGTEGSLQVAINAMITSRSPHHFVGVNADGI
                     TSIIKTTGNPDHHIVLRGGGGRTNYSVEDIARAEAAVASEGLARGVMVDCSHDNSGKN
                     HQRQVEVAGDVLKQFQRGRRSIMGLMLESHLEGGRQNWDPNKTLTYGMSITDSCLAWS
                     DTEELLYGMAESLSAKTV"
     gene            1571..2350
                     /locus_tag="PLS35_14005"
     CDS             1571..2350
                     /locus_tag="PLS35_14005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNI19832.1"
                     /translation="MLIDTHTHLDDARYASDRDAMIARAREAGVETMITIGCDLATSR
                     SAVALAGQYPFVYASIGVHPHEVKHITDSWYDELRQLAREHKVVAYGEIGLDYHYNHS
                     NPDEQRLRFREQIQLARELKLPVIIHTREAQDDTIRILKEERASEIGGVFHCFSGDAW
                     LAKDALELGFYLSFSGILTFQNATMLRDIAKTVPADRLLIETDCPYLTPIPHRGKRNE
                     PAYVKHVAETLATITSNGDTLTADDIGRLTSDNARRLFKIP"
     gene            complement(2337..2870)
                     /locus_tag="PLS35_14010"
     CDS             complement(2337..2870)
                     /locus_tag="PLS35_14010"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNI19833.1"
                     /translation="MATHLLVDGYNLLGSARMAGPAGPGRLEAARDGLLHALAGYRQR
                     KGHAITVVFDGWQGGLGAEQREFQSGVEVVYSKRGERADQVIERLARLYGRDCAVVSS
                     DHEVINAARAAGAFVIGAPDFRAKLYQKPTRHQGPAFKELDRGEAEVVKRSKDKGGNP
                     RKLPKSQRKRNQQLREF"
     gene            2934..4292
                     /locus_tag="PLS35_14015"
     CDS             2934..4292
                     /locus_tag="PLS35_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNI19834.1"
                     /translation="MVPCREAFRLVPRMNWLFRLCLLLTLGWFLAGSASLSSAEMPSE
                     PSWPQIPQPSGDAAAPQFLLIKQDSAGTSRYPIIVRDLRTWSTPDGTRLVLDLNHKAS
                     FTETHVRNPDRVVIDISNAILGKSSRQRVSGGTIPQSFHITQSRPRVVTITLARTQGS
                     KYKAFALDNPDRLVIDIYHKAKDQIRQIGETSSTSPSAPAVALPTPPTIPSPTVTEPV
                     RPVAPKPASQIQTIVIDPGHGGKDPGTIGQHGTTEKDVTLKVGMLLKDLLSTLPNTRV
                     LMTREKDAFIELEDRAKFANGKNADLFVSIHVNSHPHKGVRGLEIYHFGEAKDQRALE
                     VAARENGTPLNSTGLGVEYILADLLTTKKIEHSQELAWTAKQAMVTNLNGRYSTIDHG
                     VKTAPFYVLRFTTMPSILAEIAFMSNPAEEEQMRTQPFLAHIAESIFEGVKTYLRTNP
                     SR"
     gene            4337..5101
                     /gene="truA"
                     /locus_tag="PLS35_14020"
     CDS             4337..5101
                     /gene="truA"
                     /locus_tag="PLS35_14020"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNI19835.1"
                     /translation="MMTTFKLVLEYDGTRYSGWQRQLNASTVQAAVEAALAAISQTRL
                     TVVGAGRTDAGVHALGQVASFKTDRGLSPREWLRALNAHLPSDISALSVDAVPEHFHA
                     RYSATGKLYEYHILNRSERAALLRERAWSVYQPLNVAAMADAAAVLLGSHDFSSFETA
                     PTDNDNPHCRMQQSALRREGDLIVCSFYADRFLKQMVRSLVGTLVDVGRGKRSASDMD
                     TILKARSRPAAGRTAPPHGLYLVRVDYDAASAPPVT"
     gene            5179..5496
                     /locus_tag="PLS35_14025"
     CDS             5179..5496
                     /locus_tag="PLS35_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HNI19836.1"
                     /translation="MKHLCAVLSSIVLAAVLVTPQLSYAGAQQNKMKTCNADADAKGL
                     SGEGKGEQRKAFMKECLSAKPEKAAAGASQQNKMKTCNKDAKAKNLAGDERKKFMKEC
                     LSN"
     gene            complement(5634..6326)
                     /gene="cysE"
                     /locus_tag="PLS35_14030"
     CDS             complement(5634..6326)
                     /gene="cysE"
                     /locus_tag="PLS35_14030"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNI19837.1"
                     /translation="MSCKADDARTAIPAMLKAIAQDLQAIFDRDPAATSRLEVILTYA
                     GFHALLAYRLAHWLKQHQVPFLPRAVSQLARWLTGIEIHPSAKIGASFFIDHGMGVVI
                     GETAEVGDHVTLFQGVTLGGTGKERGKRHPTLGNHVVVGAGAKILGGITIGDNVKIGA
                     NSVVVKSVPPNSTVIGVPARIIKSQGERLPDATMDHTNMPDPTADRFAALEMELIELR
                     KKLENQDTGKPR"
     gene            complement(6323..6805)
                     /gene="ispF"
                     /locus_tag="PLS35_14035"
     CDS             complement(6323..6805)
                     /gene="ispF"
                     /locus_tag="PLS35_14035"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNI19838.1"
                     /translation="MTGVRVGCGWDIHPLVAGRKLILGGLEIPHHQGLQGHSDSDALV
                     HAICDALLGAMGEGDLGRHYPSSDQRFKNISSLKLLEDVVGKLRAKGYRLANVDSTII
                     AQAPRLSPHLAAMQKSIAGVLQIDPDLVNVKVKSGEGLDAVGREEAIAAQAVCLIERD
                     "
     gene            complement(6802..7497)
                     /gene="ispD"
                     /locus_tag="PLS35_14040"
     CDS             complement(6802..7497)
                     /gene="ispD"
                     /locus_tag="PLS35_14040"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNI19839.1"
                     /translation="MALVPAAGRGLRMGGSVPKQFLALGGRPILAQSLHVLQASPVIH
                     EIILAVPQADRQYCLDHFVAPGEFGKVTKVVSGGAQRQDSVRHALAEVSEADIILVHD
                     AVRPFLTGDMIRRVVDAAAEHGAAIIALPMRDTVKYVGAGGVIERTIDRGPLWLAQTP
                     QAFRRACLEEAHRKALLGNIHATDDAHLVELLGKPVVVVEGSGENIKVTRPEDLVIGE
                     AILQSRTATRSAE"
     gene            complement(7656..8681)
                     /locus_tag="PLS35_14045"
     CDS             complement(7656..8681)
                     /locus_tag="PLS35_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNI19840.1"
                     /translation="MVTRAIFVLLSALAGLALFLRAQDPSQGFLLVGLGMGAVAGGLI
                     LAGEYALRRLSFGIIVGGAVGLAGGLVLTGLVEWVGSAVFDVETFLFHIGGLVFLLGF
                     PYLGLAMGARFGQEQFPGVHPGQSATGSSQGCLKVLDTSVIIDGRVADLCETGFLEGP
                     FLVPHFILNELQHIADSSDSLKRARGRRGLDILNKIQKMPEVDVRIIEEDFPHVKEVD
                     AKLVVLAKKVGGRIVTNDLNLNKVAELQGVRVLNINELCNALRPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMIVVDNARRCIGRNVDVTVTSVLQTTAGRMIFTRLKEESERE
                     EYQVARG"
     gene            complement(8732..>9970)
                     /locus_tag="PLS35_14050"
     CDS             complement(8732..>9970)
                     /locus_tag="PLS35_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNI19841.1"
                     /translation="TPFEDPFFRRFFGEEWMKRFEAPKERKQQGLGSGVIVDANGLII
                     TNNHVVNKADEIKVFLSDKREFKAKLVGTDAKTDVAVLKIEATGLPTVAWADSDKLEV
                     GEFVLAVGNPFGLTQTVTLGIVSALGRAAGIAEYEDFIQTDAAINPGNSGGALVNVRG
                     ELVGINTAIYSQSGGNMGIGFAVPSNMAHSIMDQLVQHGKVVRGWLGVSIQELTPELS
                     SQFGVPKEVKGVLVSDIMDDSPAKKAGFERGDVIVEYDGKPMDSPAHLRNAVAQTVVG
                     KKVTVKLIREKKPKSIDLVIAEQPKNLSQAAAEEGGESIAPAGLLADIEVREVNSELA
                     GRYGLKSSDRGIVVVRVKPGSQAEEAGVREGDLVLEVNRKSVGTLKSYEHVAANLPKD
                     QAVLLLLKRQGRAIYLTLRP"
BASE COUNT         2147 a         3212 c         2639 g         1972 t
ORIGIN      
        1 agacgagtac aggcgccggc atgagctgcg gcggcgcatg cgccaggaac gcgcagggga
       61 ccggcacatc accgatcagt gaatgaccgg tatgatactg gacccactgg cagaggtcgg
      121 tatcggaatg ctcgtggccg tcatgatcgg cggcggccag ttcgtgatgg atctccaacg
      181 cggcggcgaa cggcgccatc gccagcagga gagtcaccag aagcagggcg atggtcgaac
      241 tgaatctcac ggaggcgtaa cggggcagca tagccatctc atcgtctaag gacacgtcgg
      301 caaacttgga ttttgtagca aagcgcccgg ttgaaagcaa ggcaatgcgc cgatcctgcc
      361 caccctctgg actttctctt tcacttttcg ctatgttaac tcacaatttt catgtcacac
      421 cttagcagag agaccgttca aagggaccat catgaatagg ccgatcgata accagcatgt
      481 catagagatt aaacccctcc cctcgccccg cgatgtcaaa acgcagctgc ctatcagcga
      541 cgaggtatcc gagctggtct tccagacgcg acaggcgatc cggaatatcc tgcacggacg
      601 tgacatggac cgcctcattg tggtggtcgg cccctgctcc attcatgatc ctgatgccgc
      661 ctacgaatac gctgaccggc tcaaacccgt cgctgaggcc gttcgcgaca aactgctgat
      721 cgtgatgcgc acctatttcg agaagccacg aacgactgtg ggttggaagg gtctcatcaa
      781 cgaccctcac cttgacggca cctgcgacat tgctcagggc attcagctgg cccggacgat
      841 tttgctgaac atcaatggca agggccttcc ctgcgccacc gaactgcttg atcccgtcac
      901 gccgcaatac attgccgacc tcctgagctg gaccgccatc ggagctcgga caactgagag
      961 ccagatccac cgtgaaatgg ccagcggcct gtcgatgccg gtcggattca agaacggcac
     1021 ggagggcagc ctgcaggtcg ccatcaacgc catgatcacc agccggagtc cccatcattt
     1081 tgtcggggtg aacgccgacg ggatcacctc aatcatcaaa accaccggga atcccgatca
     1141 tcacatcgtc ttgcgcggcg gcggcggacg cacgaactac agcgtcgagg acatcgctcg
     1201 cgccgaagcg gccgtggcaa gcgaagggtt ggcccgcggc gtaatggtgg actgttcgca
     1261 cgacaactcc gggaagaacc atcaacgcca agttgaagtg gccggcgacg tcttgaaaca
     1321 gttccaacgg ggccggcgct ccatcatggg tctcatgctg gaaagtcacc tggaaggcgg
     1381 ccggcagaac tgggatccga acaaaacttt gacgtacggc atgtcgatta ccgattcctg
     1441 tctcgcctgg agcgacaccg aagagttgct ctacggtatg gctgaatccc tctcagccaa
     1501 gacagtctag gctcacatcg ccctgtcact tcgatttcca ctgtcacagc cggatgccgg
     1561 aatccactcg atgttgatcg atacccatac ccatctcgac gatgcgcggt acgcctccga
     1621 ccgcgacgcg atgattgcca gagcaaggga agcgggtgtg gagacgatga tcacgatcgg
     1681 ctgtgatctc gcgacaagcc gatcggcggt tgccctcgcc ggtcagtacc ctttcgtgta
     1741 tgcctcgatc ggcgtccatc cgcacgaggt caaacacatc acggacagct ggtatgacga
     1801 actgcggcaa ttggcgcgag agcacaaggt cgtggcctat ggcgagatcg gcctcgatta
     1861 ccactacaac cactccaatc cggatgagca acgcctccga ttccgtgaac agattcaact
     1921 cgcccgggaa ttgaagctcc ccgtcatcat ccacactcgg gaagcgcagg acgataccat
     1981 tcgcatcttg aaggaagagc gggcgtcaga gatcggcggg gtatttcact gcttttccgg
     2041 agacgcctgg ctggcgaagg atgcgctgga gttgggattc tacctgtcat tttccggcat
     2101 tctgacgttt cagaatgcga ccatgctgcg ggacattgcc aagaccgtcc cggctgaccg
     2161 gctgctcatc gaaaccgatt gtccctacct caccccgatt ccccatcgag gcaaacgcaa
     2221 cgagccggct tacgtcaaac acgtcgccga aacgttggcc acgatcacgt ccaatggcga
     2281 cacgctgacg gcggatgaca tcggccgcct caccagcgac aatgcgcgcc gcctcttcaa
     2341 aattccctga gttgctggtt ccgcttgcgc tgagacttgg ggagcttgcg gggattcccg
     2401 cccttgtcct ttgatcgttt cacgacctca gcctcgcccc gatccaattc tttgaatgcc
     2461 ggaccctggt ggcgcgtcgg cttctgatac aacttcgcgc ggaagtccgg cgcaccgatc
     2521 acgaaggccc cggcggccct tgccgcattg atcacttcat gatccgatga caccaccgcg
     2581 caatccctgc catacaatcg tgccagacgc tcgatcacct ggtcggcgcg ttcgccccgc
     2641 ttggaataga ccacctcgac gcctgattga aactcgcgct gttcggcgcc cagtcctccc
     2701 tgccatccat cgaacaccac cgtgatcgca tgccccttgc gctgacggta gccggccagg
     2761 gcatggagca ggccgtcgcg cgccgcttcg aggcggccgg gaccggctgg acccgccatg
     2821 cgcgcgcttc ccaacaggtt atatccatcg acaagaagat gtgtcgccat ctacaattac
     2881 gcagtttgag accttttgtt gacaatacca taaacatctg agaaaaggcc tagatggtac
     2941 cttgtcgaga agcgttccga ctcgttcctc ggatgaattg gctgttccgt ctttgcctcc
     3001 tgctgaccct cggctggttc ctggctggtt cggcttccct gtccagcgcg gagatgccgt
     3061 ccgaaccatc ctggccccag attccccagc cgtccgggga cgcagcggca cctcaattcc
     3121 tgctgatcaa acaagattcc gccggcacct cgcggtatcc gatcatcgtc cgcgacctgc
     3181 gtacctggtc cactccggac ggcacacgcc tggtgctcga cctgaatcac aaagcctcct
     3241 tcaccgaaac ccacgtgcga aatcccgatc gggtcgtcat agacatcagt aatgcgattc
     3301 tgggaaagtc ctcccggcag cgtgtctccg gcggcacgat tccgcaatcg tttcacatta
     3361 cgcaaagtcg tccgcgggtc gtcaccatca ccctcgcgcg cacccagggc tccaagtaca
     3421 aagcgtttgc cctggacaat ccggaccgcc tcgtcatcga catctaccac aaggccaaag
     3481 atcagatccg gcagatcggc gagacgtcct cgacgtcgcc atcagcccct gccgtggccc
     3541 tcccgactcc gccgacgatt cccagcccca cggtgacgga accggttcgc cccgtggcgc
     3601 ctaaacctgc cagtcagatt cagacgatcg tgatcgatcc cggccatggc ggaaaagacc
     3661 ccggcaccat cggacaacat gggacgacgg aaaaagatgt cacactcaaa gtcggcatgt
     3721 tgttgaagga cctcttgagt accttgccca atacacgcgt gctcatgact cgcgagaagg
     3781 atgcgttcat cgagttggag gatcgggcca agtttgccaa cggcaaaaat gccgatctct
     3841 tcgtgtccat tcacgtcaac tcgcacccgc ataagggggt gcgcggcttg gagatctacc
     3901 acttcggcga agccaaggat cagcgggcgc tggaagtcgc ggctcgggaa aacggcacgc
     3961 cgttaaacag cactggactc ggcgtggagt acatccttgc agatcttctg acgaccaaga
     4021 aaatcgagca ttcacaagaa ctggcctgga cggccaaaca ggcgatggtc accaatctca
     4081 atggtcggta tagcaccatc gatcatggcg tgaagaccgc gccgttttat gtcctgcggt
     4141 ttacgaccat gcccagcatt ctcgcggaaa tcgccttcat gtcgaatccg gcggaagagg
     4201 aacagatgcg cacgcaaccg ttcctggcgc atatcgcgga atcgatcttc gaaggggtca
     4261 agacatacct ccgcaccaat ccctcccgat gacagccgcc ttcccgttaa gctagagtgc
     4321 tgcttttcgc actcacatga tgaccacgtt caagctcgtt ctcgagtacg atggaacccg
     4381 ttactcaggc tggcaacgcc aactgaatgc atcgaccgtt caggctgcag tggaagcggc
     4441 gcttgcggcg atctcgcaaa cgaggcttac cgtggttggg gcaggtcgca cggatgccgg
     4501 cgtgcatgcc ctcgggcagg tggcgagctt caaaaccgat cgcggccttt cgccacgaga
     4561 gtggcttcgc gccttgaatg cccatctccc ctcagacatc agcgcgttgt cggtcgatgc
     4621 cgtgccggag cattttcacg cccgatattc ggccaccggc aaactctacg agtaccacat
     4681 cctgaatcga tcggagcggg cggctctgct gcgggaacga gcctggtctg tttaccaacc
     4741 actcaatgtc gccgcgatgg ccgatgccgc agccgtcctg ctcggctcgc acgatttctc
     4801 ctcgtttgaa accgcgccca ccgacaatga caatccgcac tgtcgcatgc agcaatcggc
     4861 actccgccgg gagggcgatc tcatcgtgtg ctccttctat gccgaccgtt tcctcaaaca
     4921 gatggtgcgc agtctcgtgg ggacgctggt ggacgtagga cgcggcaaac gatcggcgtc
     4981 cgatatggac acaattctca aggcccgctc ccgccctgca gccgggagga cggccccgcc
     5041 ccatgggttg tatctcgtca gagtggatta tgacgcggca tccgctcctc ctgtcaccta
     5101 ggtaactgtc ccgtgagaca gaaccggcaa tttttgctat agttccgccg accccgtcca
     5161 cctcaacagg agacttccat gaaacatctt tgcgcagtct tgtcctcgat cgtgttggca
     5221 gccgtgctcg tcacgccgca actcagctat gccggagcac aacagaacaa aatgaagacc
     5281 tgcaatgccg atgcggatgc caaggggctc agcggggaag gcaaaggcga gcagcgcaag
     5341 gcattcatga aagaatgtct ctccgccaag ccggaaaaag ccgcagccgg ggcgtctcag
     5401 cagaacaaga tgaagacctg taataaggat gccaaggcaa agaatctggc cggcgatgaa
     5461 cggaagaagt ttatgaagga atgcctgtcc aactaaccgc agcgaccccg gcatggtact
     5521 aggctgtgcc ggggttcatc gatcacccac acaacatccc gtcttcgccg aagcagtcgg
     5581 cgtggtacct gtctccaatc gcacatcaat agaggaacga gcgtcgcggc gggttagcgg
     5641 ggcttaccgg tgtcctggtt ttcgagtttc tttcgtaact cgatgagttc catctccagc
     5701 gcggcaaagc ggtctgctgt aggatccggc atattggtat gatccatcgt ggcgtcggga
     5761 agccgctcgc cttgtgattt gatgatcctg gccggcactc caatgacggt cgagttcggc
     5821 ggaaccgatt tgacgacgac cgaattggcg ccgattttca cattgtcgcc gatggtgatg
     5881 ccgccgagaa tctttgcacc cgccccgacc accacatgat tccccagcgt gggatggcgc
     5941 ttgccccgct ctttgcctgt gccgcccagc gtcacgccct gaaacaaggt gacatggtcg
     6001 ccgacttccg cagtttcacc gatcaccaca cccatgccgt gatcgataaa gaaggaggca
     6061 ccgattttgg cagagggatg gatttcgatt ccggtcagcc aacgcgccag ttgtgacacg
     6121 gcgcgcggta agaacggcac ttgatgctgt ttcaaccaat gcgcaagccg gtaggccagc
     6181 aatgcgtgga atcccgcata ggtcaaaatg acctcgagcc tgctcgtcgc ggcaggatcc
     6241 cggtcgaaaa tcgcctggag gtcttgagca atggccttta acatagctgg gatagccgta
     6301 cgcgcgtcat ccgccttaca actcagtcac gctcgatcag acagacggct tgcgcggcga
     6361 tcgcttcttc gcgacccacc gcatccagcc cttcaccact cttcactttc acattcacca
     6421 gatcgggatc gatttgcagc accccggcaa tcgacttttg catcgcggcc aaatgcgggc
     6481 tcaagcgagg cgcctgggca atgatggtgc tatcgacatt cgcaagacga tagccttttg
     6541 cccgcaattt gcccacgacg tcttcgagca acttcaagct ggagatattc ttgaaccgct
     6601 gatccgagct cggatagtgg cggcccagat ctccttctcc catcgccccc aacagggcat
     6661 cgcagatcgc atgcacgagc gcatcggaat cggaatgtcc ctgcagcccc tggtgatgcg
     6721 gaatttcgag tccgcccagg atcagcttgc gtccggccac caagggatga atatcccacc
     6781 cacaccccac gcgtacaccc gtcattctgc actcctcgtt gctgttcgtg attggagaat
     6841 cgcctcgccg atcacgagat cttccggcct tgtcaccttg atattttcac cgcttccctc
     6901 gaccaccacg accggctttc ccaacaactc gacgagatgc gcgtcatccg tcgcatggat
     6961 gttgccgagc agcgccttcc gatgcgcctc ctcaaggcag gcacgccgga aggcctgcgg
     7021 ggtttgcgca agccacaagg gaccgcgatc gatggttcgc tcgatcacgc cgccggcccc
     7081 gacatatttc accgtatccc gcatcggaag tgcaatgatg gccgctccat gctccgccgc
     7141 tgcgtcaacc acacggcgta tcatgtcccc ggtcagaaac ggccgcaccg catcgtgcac
     7201 gagaataata tccgcctcac tcacctcggc caacgcatga cgaaccgaat cctgccgttg
     7261 cgctccgccg gacacgacct tggtgacctt cccgaattct ccgggcgcca caaagtgatc
     7321 cagacaatat tggcggtccg cttgcggcac ggccaaaatg atctcatgaa tgaccggaga
     7381 ggcttggagg acgtgaagag actgcgccag aatcggccgt ccgcccaggg cgagaaactg
     7441 cttagggact gatccgccca tgcgaaggcc acgaccagcc gccggaacaa gggccactgt
     7501 tctcaatccc gtcggtgacg atttcgactc aggaaccgac acggattgac tcacagaaca
     7561 agaacacagc gcaacctgcc aaccgaatcc acgacctcat gcgcaggcgc cggcccccgg
     7621 aaggggcgag gctaccgcgc ctgctcatca acgggctaac cacgcgcgac ctgatattct
     7681 tcgcgttcgg actcctcttt caggcgagtg aagatcatcc gccctgccgt cgtttggagc
     7741 acactggtga ccgtgacatc cacattgcgt ccgatacagc gccgcgcatt gtcgacgacg
     7801 atcatcgtgc cgtcatccag gtacgccacg ccctggccgg cttctttgcc ttccttcaac
     7861 acgaagacgc gaatggtttc acccggcaag accaccggcc gtaaggcgtt acacagctca
     7921 ttgatattca acacgcgcac gccttgcaat tcggcgactt tattcagatt caagtcattg
     7981 gtgacaatgc ggccccccac ttttttggcc aacaccacca gtttggcatc gacttctttg
     8041 acgtgaggaa aatcttcctc gatgatacgc acatcgactt cgggcatttt ttgaatcttg
     8101 ttcaggatat ccaaccctcg ccgcccgcgt gcacgtttca acgaatcgga tgagtccgca
     8161 atgtgttgca attcattcaa aataaaatga ggcaccagga aaggcccttc gagaaatccc
     8221 gtttcgcaga ggtccgctac ccgaccgtcg atgatgacac tggtatccag cactttgagg
     8281 catccctggg aagaccctgt cgcggactgc cccggatgaa ccccgggaaa ttgttcctgg
     8341 ccgaaccgcg ctcccatcgc taacccgaga taaggaaatc ccagcagaaa caccaagcca
     8401 ccgatgtgaa ataaaaatgt ctcgacgtca aagaccgcgc tgccgaccca ctcaaccaac
     8461 cccgtcagca ccaaaccacc ggcaagccca acggctccac cgacaataat accgaatgac
     8521 agcctccgga gggcatattc cccggcgagg attagtccac cagctacggc ccccatgccc
     8581 aaccctacca gcaggaatcc ctgactggga tcctgggccc gcagaaacaa ggccagcccc
     8641 gcaagagcgc tgagaagaac aaatatggcc cgtgttacca tactgttttc ctcctctctg
     8701 tgatcagcct cgaatcaacg actcgaagca atcacggcct cagcgtcaga taaatggccc
     8761 gcccctgtcg cttcaacagg agcaagaccg cctggtcttt tggcaaattt gcggccacat
     8821 gttcgtacga cttgagcgtg cccaccgact tgcgattcac ctccaacaca aggtcgcctt
     8881 cgcgcacacc ggcctcttcc gcctgactgc ccggcttgac ccggaccacc acaattccgc
     8941 ggtcgctgga cttgagccca tatcggccgg ctaactctga attcacctcc cgcacttcga
     9001 tatccgcgag cagtccggct ggcgcaatgg actccccgcc ctcttctgca gcggcctgcg
     9061 acagattctt cggctgctct gcaatgacca ggtcgatgga cttcggtttc ttctcacgaa
     9121 tgagcttcac cgtgactttt ttcccgacca cggtctgagc cacggcattt cgtaagtggg
     9181 ccggcgaatc catcggcttg ccgtcatatt cgacaatcac atctccacgc tcgaacccgg
     9241 cctttttcgc cggactatca tccatgatgt cgctgacgag gacgcctttc acctctttcg
     9301 gcaccccgaa ctgcgaggac aattcaggcg tcaactcttg aatggaaacg cccaaccagc
     9361 cgcgcacgac tttgccatgc tgaaccagct ggtccatgat ggaatgggcc atgttgctcg
     9421 gcaccgcgaa tccgattccc atgtttccgc cgctttgact gtagatggcg gtattgatgc
     9481 cgaccaactc tccgcgcacg ttcacgagtg cgcccccgga atttcccgga ttgatggccg
     9541 catccgtctg gatgaagtct tcgtattcgg cgattcctgc cgcgcgcccc agcgcgctca
     9601 cgatgcccag ggtgacggtc tgagtcagac cgaaaggatt gcccactgcc agcacgaatt
     9661 cgccgacttc cagcttgtcc gaatcggccc atgccacggt cggcaggccg gtcgcctcga
     9721 ttttcaggac ggcgacatcg gtcttcgcat ccgtcccgac gagcttcgct ttgaactccc
     9781 gcttgtccga caaaaacacc ttgatctcgt cggccttatt caccacatgg ttgttcgtaa
     9841 tgatcaaccc gttggcatcg acgatcacgc cggaacctaa cccctgctgt ttccgctcct
     9901 tcggcgcttc aaagcgcttc atccactcct cgccgaagaa gcgtcgaaaa aagggatcct
     9961 cgaacggtgt
//
LOCUS       DAOQEW010000142         9841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2183_length_9841_cov_6.364500, whole genome shotgun sequence.
ACCESSION   DAOQEW010000142 DAOQEW010000000
VERSION     DAOQEW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_9841_cov_6.364500"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..2191)
                     /gene="traN"
                     /locus_tag="PLS35_14055"
     CDS             complement(236..2191)
                     /gene="traN"
                     /locus_tag="PLS35_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HNI19842.1"
                     /translation="MKRMLIGICLAICLVCGVVEVRADCSGIDDPYLKAKCEVEGQKA
                     SGLVGAGILAPNMTKVPQYSGSPGCVTAGCSGAAEERYYDDPGSLSAAGGAAASSDER
                     YSKIQQSRIDRDGWDLRTSSPVTTAQSTAATLPTDSIMTESCSVVNMCTSYTEGAGVT
                     GTCQVPGGSRQTCLKIRNPTLLPNACTVPGIPSPPYTSETEVNGCKDLEAAGASGLAA
                     LVTTTCLDGAARDLKCAQPYATVLTGGTTGFNAHDHAPGMAMGNFTIESAFPIWDGTR
                     WVVGLNVTGNSAGEDKYGCTNVGSVKTGPYVLTPANPTASGNGSPAYCNSKCSCFGYP
                     MTFTMGAFSPTGPLSWQVPFTFTYESHSIAVMVTGTFLDTYSVAPLTGCFQEEQTWDV
                     TSTAGDTCGEYRDKGCNQVSSRCASVQPGTGYCLMYENTYKCPGPSECSTTQPITQCT
                     KCGKPDSPVPFCVDTSTPPNENLALAATWLAMTKNVEEDWDPDRLRIFTGKRSTCDFS
                     TIGRSLINCCDSDPDKLIGKCSEEEIALARDKHDRKAHLIGTHCVDKGSFLVGSVCLR
                     KEEVYCTMKSELGRMVQEQGRVQLGMTWGSADAPQCDGFTVDQFSALNFQAMDFTEWY
                     KNVSANIDPAVITKEMATKICTYTGTC"
     gene            complement(2208..2699)
                     /locus_tag="PLS35_14060"
     CDS             complement(2208..2699)
                     /locus_tag="PLS35_14060"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNI19843.1"
                     /translation="MKILTVDVIPGHTYRYFQALIQIETVGIASWRDAVVRFTDFWGG
                     RAKTYDSPIVHATQSMVNRLAQSAAALGADLIIGFDIQIHQVTARYWGWGMAQIIMRG
                     TPIQFMDRDALGTILAGELSPFVARDQSARHPPDGNSLGEAVGMDEMTVPSLDTARLG
                     TRR"
     gene            complement(2706..3677)
                     /locus_tag="PLS35_14065"
     CDS             complement(2706..3677)
                     /locus_tag="PLS35_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19844.1"
                     /translation="MWQRMAALSLALLSGCVSTGEYQDLETKYQHQLAVKEVMETEHD
                     ELSQRVDNVSQVYRHVAQEQMGLKAQAEKLQGDILTVKGVVKDTQNQYESQRAQFAQQ
                     AQQLSGVDARLQNIAGKIETLTETTVALANRFERVELSIGKLGKSLQARGGAPDVQKT
                     RVQLGEGKSGSAPKTEPVAKAGSPNMEGSVGAGGSGVEPSGTSLAVAPSPVATTTVPA
                     KPDVPMPVPQRVIEAKVESPRTKPDADKGWLRRFLDGFRGGNGQNAELVSPPLASGMS
                     GKASTGRPLLPAVDGEVMAAPMNTLTISIDADAPKKAEVSPSDTEKR"
     gene            complement(3722..4366)
                     /locus_tag="PLS35_14070"
     CDS             complement(3722..4366)
                     /locus_tag="PLS35_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19845.1"
                     /translation="MAHGVDAERQLGMVRREGQSLRSASPTNGRRPDRAEENKRRLED
                     PNSVLLLPQTKAGMACVSMLYMFDKVIHRLRRAAGSQLSIEQVRNELARVEQWVARAK
                     VILRELGVGEFELLPQDPYPDLKERSVKAAWRNARVITPRVGDTVRLVPVIRALDNAL
                     NSIRLRAEDLTPYVKHFQAVDELVVALHDVIGHVARVTVTEYEPRGAVKAFLNQ"
     gene            complement(4382..5011)
                     /locus_tag="PLS35_14075"
     CDS             complement(4382..5011)
                     /locus_tag="PLS35_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19846.1"
                     /translation="MARVIRMDPGHSAYLLWADSIGRYAILPHQYADRQYKVRDSLMG
                     AVYKVGDEFPMLSQRSMHYFRRIAELVLAPVLADDHVHIKRVATAQAASFVKIAVETY
                     RTGEDVIATCKPLLYEFRTYTSRTIALVKYHMEVEAYIREALAPAPSRSVRRVDVYRE
                     EHQARVIVERGTIAQFLGRKGMNAAVASKLTGYGLVLVDEGDLGKTARA"
     gene            5229..6416
                     /locus_tag="PLS35_14080"
     CDS             5229..6416
                     /locus_tag="PLS35_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19847.1"
                     /translation="MVLLVLVTLVSCAPVFTPTTLQNFKESRETSSTTAVERPRLALS
                     PHSDGLGWTVRVTQTVEEKNRIRREEVWGGYTYERRLNLVTMVGGGLLCAPNLLLNTL
                     FSMGTPNQPGWSSPWQYCLAAAGYDVPGRADTRTRIADDLDPQVQTVTRIIVDGDLRL
                     TFAADGRDRIGIAVPVSKDSQGTHIRLRWLAMALREQGVNPADVPDGTVEISYNPTVG
                     NPGPTTQLPIDGHVLTAMLQHDLAVLPQDRWPKQPRLRLAVSNDWGHEQSQLIVDRLI
                     GRMKERGLPLVIRNERYRKLVPVQVRQLAPFFNDERQPSAGMGTGANLLVSIDIASAS
                     DQGLLLTTTLVSVETAQVLAQFVLECPINAVAPTAETLAAVVTDLVLPHGPTLTRGWF
                     VTP"
     gene            6413..7537
                     /locus_tag="PLS35_14085"
     CDS             6413..7537
                     /locus_tag="PLS35_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19848.1"
                     /translation="MTRYGTFLICFLATSAVGCSSLILSNTPVQGENTSTREIARTPI
                     DVPEVTLTPHHDQQRLTIRVTQRSQITLETDTPMQVEARRYVFSPTAPLAGAVHCPVL
                     TVAYVISAGHLGSQSLAPACRRLLMQEPLAGSPRVAAEIEHRYETRTTTEPVRYATLR
                     VAWDDAIHPPLVYDVNPDGEVSIPQHALRSGTKRDPSTLPTLSILVDNRVLWTHRLTP
                     SEMTPSGPLPDEPHTAGTSWPPIVALVLEPLDGAAPPALRAWGRLVTQALLERHICVV
                     APITFAKDLQDELLLHQSGRISDQTAVHFGKWIPATVALTIRNGAANQGIAILEFVDI
                     QRAERIRYDVLRYSSDWKEDAQMLKAYMQTVFPKGYQSCS"
     gene            complement(7976..>9841)
                     /locus_tag="PLS35_14090"
     CDS             complement(7976..>9841)
                     /locus_tag="PLS35_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19849.1"
                     /translation="PAFQANALLYDGQVSFFDGREGGTKSISGERNLVDGTHLSGQFT
                     QLNVMGQDGHQVEMFHGYAQQPGAPARDVTGLIDNGSLVSFKGASGNEFRDVIERTDS
                     GDLQSIGAQETYTAPGGTHSLSNFQGVTVNPQGDAFKRSSTNLDGVSVYSDQMRGSRQ
                     MTVEFEQSLRGYNNEVQSIDKESGWRRINDGHRDMDVYANLYYGPTSSETGERKLLGG
                     TITNVTDNTYSTFMKDGEGNEVWGIARSLEDPNSGAMSGSIDQTVKVVAAKNGAIDTQ
                     VLGPMKDDGGQREVLYHSVLGGLNSQYYNNERKSGIFTVPDPMHPGQTKELQGTFYYS
                     PQSGQLIASEVTDLQTGKIGQYRTDAQGQEHFQVVEYSQSPDGASVIANTKELSKNEY
                     AAHGFAVNDVLDGSGNVLSSSGHKGSDFHDFNRVQFHHDSGANLTITQMAFAAKGTDI
                     TNPTQTDEAIAYGLEFGRIGMDGASRYLILKGRMGKFGGGGVGGTPAQPGGGPVPNGP
                     GGVGGPSGLLDSHQNMGAWWKEYSGVIDNAISFARSSVEHLEKTGRAQSMSPEELQNF
                     AANAAYNAVRTSALGMMGEEASSAFGTMPRWQEGQESLLKISKPSSPGVNTGSNK"
BASE COUNT         2123 a         3109 c         2384 g         2225 t
ORIGIN      
        1 ggatcccctt cactctccac tagaataatc gcttccaata tttgtacggt cactgcgtaa
       61 cgattcgccg ccgccacaac acaactgtgg ttcacatacc aaaaccggtc cggaggcatc
      121 tccgccgcaa cactgtccgg caagacaccg agacatacca ccgcaagaca gaaccacgtc
      181 gaacggcgac ttttcggtct ggagaatgca cccagaggcc caccataccc ccgtcttaac
      241 aggtgcctgt gtaggtgcat atcttcgtcg ccatttcctt cgtgatcaca gccgggtcaa
      301 tattcgctga aacatttttg taccattcag tgaaatccat cgcttggaag ttcaaagcgc
      361 tgaactgatc gaccgtaaat ccgtcacact ggggcgcatc cgctgatccc caggtcatgc
      421 ccaactgcac acggccttgt tcctgcacca tccgaccgag ctcggatttc atagtgcagt
      481 agacctcttc ctttcggagg cacacactgc cgaccagaaa ggagccttta tccacacaat
      541 gtgtcccaat tagatgagcc tttcgatcgt gtttatccct cgctagcgcg atctcctcct
      601 cagagcattt cccaatcaat ttatcggggt ccgaatcgca gcaattgatt agtgatcgcc
      661 cgattgtgct gaaatcacag gtgctccttt tgcccgtgaa gatacgcaga cggtccggat
      721 cccaatcttc ttcgacattc ttggtcatcg ccagccaggt ggccgctaac gccagattct
      781 cattgggtgg cgtactggta tccacgcaaa acggcacagg actgtctggt ttcccacact
      841 tcgtgcattg agtaatcggt tgcgtcgtcg aacattccga cggtcccgga cacttgtagg
      901 tgttctcgta catcaagcaa tatcccgtcc ccggctgcac cgatgcgcag cgagagctga
      961 cttgattgca gcccttgtct cgatactcgc cacaggtgtc cccggccgtg gacgtgacat
     1021 cccaggtttg ctcctcttga aagcatcccg ttaagggcgc cacgctgtac gtatcaagaa
     1081 aggttcccgt taccatgacc gcaatcgaat gcgactcgta agtaaatgta aacggaactt
     1141 gccagctgag cgggccggtg ggactgaagg cgcccatcgt aaacgtcatg ggatacccaa
     1201 agcatgagca cttgctattg cagtaggccg gcgagccatt tccagaggcc gtcgggttcg
     1261 ccggagtcaa cacgtaaggc ccggtcttca ccgagccaac atttgtgcag ccgtatttgt
     1321 cttctccggc ggaattgcca gtgacgttca agccgaccac ccatcgagtt ccatcccaga
     1381 taggaaacgc cgattcgatc gtaaaattgc ccatggccat tcccggcgca tggtcgtgcg
     1441 cattgaatcc agtagtcccc ccggtcagca cggtggcata cggttgagca cacttgagat
     1501 cccgcgcagc tccatcgaga caggtcgtag tcaccagtgc ggcgaggcca ctggcgccag
     1561 ccgcttctag atccttgcac ccattgacct ccgtctcaga ggtataggga gggctgggaa
     1621 ttccaggaac cgtacacgcg ttcggtagca gggtggggtt gcgaatcttg aggcaggtct
     1681 gccgtgagcc cccaggcact tggcaggtcc ccgtgacgcc agccccttcg gtgtaactcg
     1741 tgcacatatt caccacggaa cagctctccg tcatgatgga atcggtcggc agcgtcgctg
     1801 ccgtcgattg cgccgtcgtc accgggctac tagtccgtag atcccaccca tctcggtcaa
     1861 tcctgctctg ttggattttc gagtaacgct catcggagct cgccgccgca ccgccggccg
     1921 cgctcaaact gccaggatca tcgtaatacc tctcttcagc agccccagag caccctgccg
     1981 tgacacaacc cggcgaccct gagtactgcg gcactttggt catgttcggc gccagaatac
     2041 ctgcaccgac taatcctgag gccttctgcc cctctacctc acacttcgct ttgaggtacg
     2101 gatcgtcaat cccactgcaa tctgcgcgta cctccacaac accacacacc aaacagatcg
     2161 cgaggcaaat ccctatgagc atgcgcttca tcacagacag cctcccccta tcgacgcgtg
     2221 cctaaccgag ccgtatccaa tgagggcacc gtcatctcgt ccatccctac cgcttctccc
     2281 agtgaatttc cgtccggcgg atgtcgagcg gactggtccc ttgcaacgaa gggggacagt
     2341 tcaccggcca aaatcgtccc aagcgcgtca cgatccataa actgaatcgg cgttccccgc
     2401 atgatgattt gtgccatacc ccacccccag tatcgtgccg tcacctgatg aatctgaatg
     2461 tcgaaaccaa tgatcaggtc agctcccaac gccgcagcgc tttgcgcaag cctattcacc
     2521 atgctctgag tcgcatggac gatcggactg tcatacgtct tcgcgcgccc tccccagaaa
     2581 tccgtgaatc ggacaacggc atcccgccag gaagcgatcc cgacggtctc gatctgaatc
     2641 aatgcctgaa aataccgata ggtatgtccg ggaatgacat ccaccgtaag aattttcatt
     2701 cgggcctatc gcttttcggt atcagatggt gacacctcag ccttcttcgg agcatctgcg
     2761 tcgatactga tcgtcaatgt gttcatcggc gcggccatca cttccccatc gacagccgga
     2821 agaaggggac gtccagttga agcttttccg gacatcccag aagctagagg aggagacacc
     2881 agttccgcgt tttgcccatt ccccccacgg aagccatcca gaaaacgtcg taaccatcct
     2941 ttgtccgcat cgggcttggt tcgaggcgat tcgaccttag cctctatcac cctctgagga
     3001 acgggcatcg gcacatccgg tttggcaggg acagtggtcg tcgccactgg acttggagcg
     3061 accgccaggc tcgtcccgct cggttcaaca cccgagcccc cagcgcctac gctcccctcc
     3121 atattcggac tgccagcctt ggcgacaggt tccgttttgg gagcacttcc tgacttaccc
     3181 tcacccaact ggacacgcgt tttctggacg tcgggagcac cccctctagc ctgcaaggac
     3241 ttcccaagtt tcccaatcga cagttcaaca cgttcgaagc gattggctaa ggcgaccgtc
     3301 gtttctgtga gggtttcgat cttccccgca atattttgca ggcgcgcatc gactccggac
     3361 agttgttgcg cctgctgcgc aaactgcgcc cgctgggact cgtactgatt ctgagtgtct
     3421 ttcaccaccc ccttcacggt caggatgtcc ccctgcagtt tttccgcttg ggcctttagt
     3481 cccatctgct cttgcgcaac atgccgatac acctgggata cattgtcgac tcgctgggac
     3541 agctcgtcgt gctcggtctc catgacttcc ttcaccgcga gttgatgctg gtacttggtt
     3601 tccaaatcct gatattcccc ggtgctgacg caaccggaca acagtgcgag actaagggct
     3661 gccatgcgtt gccacatacc gtgctccttt catacgagag gggtgactac ctcccttttc
     3721 cctattggtt cagaaatgct ttcaccgcgc cccgcggctc atactctgtc accgtcacgc
     3781 gagcgacatg cccgatgaca tcgtgcaagg ccactacaag ctcatcgacg gcctgaaaat
     3841 gtttcacata cggagtgaga tcttccgctc ggagacgaat gctattgagc gcgttatcta
     3901 gggcgcgaat caccggcacc agtctcaccg tgtctccgac gcggggcgta atgacacgcg
     3961 cattgcgcca cgccgccttc acgctccgtt cttttaggtc gggatatggg tcttgaggga
     4021 gcaactcgaa ctcccccact ccaagctctc gcaagatcac ctttgcccgc gccacccatt
     4081 gctccacacg agccaactcg ttgcgcactt gctcaatgga gagctgagat cctgctgccc
     4141 gccgaagacg atgaattacc ttgtcgaaca tgtacagcat ggagacacag gccatccccg
     4201 cctttgtttg tggtaaaagg agcaccgaat tcggatcttc cagccgccgc ttgttctcct
     4261 ccgcccgatc gggccttcgt ccattcgtcg gagacgcgct ccgaagagac tggccctcgc
     4321 gccgtaccat ccccagttgt cgctctgcgt ccaccccatg agccatgcct cacctcctcg
     4381 attacgcccg agctgtcttc cccagatccc cttcgtccac gagcaccagt ccgtatccgg
     4441 tcaatttgct agccaccgca gcattcatcc ccttgcgacc aagaaactgt gcgatcgtgc
     4501 cccgttcaac aatgactcgc gcctgatgtt cttcccgata cacgtccact cgtcgcacgg
     4561 accgactcgg ggcaggcgct aatgcctcgc gaatgtaggc ttccacctcc atgtgatact
     4621 tcacgagtgc gatcgtgcgg gatgtgtagg tccggaactc gtacaacaga ggcttgcagg
     4681 tcgcgatgac atcttccccc gtgcgataag tctcgaccgc aatcttcaca aacgacgcgg
     4741 cttgagccgt cgccacacgc ttgatgtgca catggtcgtc cgccaacacc ggagccagca
     4801 ctaactccgc aatacggcgg aagtagtgca tcgatcgctg cgacagcatc ggaaactcgt
     4861 caccgacctt gtacacagct cccatcaatg agtcgcgtac cttgtactga cggtcggcat
     4921 actgatgcgg caagatggca tacctcccga tggaatccgc ccacagcaga tatgcgctgt
     4981 gccccggatc catccgaatg acccgagcca ttactaagtc tcccacctgc accattcgtc
     5041 actcgcctgt gctatagttt ttcgacccgt atacgcacct gccacatgcg tgtagttcga
     5101 gactcggtct cacaactatg cccaacaggt attaccggat cactcccact ccgcttctat
     5161 cctcatctag agagaagatc cgctctcttg cgtaccttgc ttggcagtgg tctagtcgga
     5221 cgatcagcgt ggtcctcctc gtgctcgtca ccttggtatc ttgtgccccc gtcttcacgc
     5281 cgaccacttt gcagaacttc aaagaatcgc gtgaaaccag ctcaacgacg gctgtagaac
     5341 gtcctcgatt ggcactatca ccccacagtg atggcctggg atggacggtg agagtcaccc
     5401 aaaccgttga ggagaagaat cggatcaggc gagaagaagt ctggggagga tatacctatg
     5461 aacgtcggct caatcttgtc acgatggtgg gagggggact actctgtgct ccaaatctcc
     5521 tgttgaatac cctcttttcc atgggaacac caaaccagcc gggatggagt tcaccatggc
     5581 aatactgcct ggccgccgcc ggatatgacg tgcctggccg cgccgatacc cgaactcgga
     5641 tcgctgatga cctcgatccc caggttcaga ctgttacgcg gataattgtg gacggtgacc
     5701 ttcggctcac ttttgccgcc gatggacggg accgcattgg gattgccgtc cctgtgagta
     5761 aggattctca agggacgcac atccgccttc gatggcttgc aatggcttta cgggaacagg
     5821 gcgtgaatcc tgccgacgtc cctgacggga ccgtcgagat ttcctacaat ccaactgtcg
     5881 gcaaccccgg cccgaccaca cagttgccga tcgacgggca cgtactcacc gccatgctgc
     5941 agcacgatct cgcggtgctc ccccaagatc gctggccgaa gcagcctcgc ctgaggctgg
     6001 cggtcagcaa tgactgggga cacgaacagt cccagttgat agtagacagg cttattggcc
     6061 gtatgaagga gcgcggcctt ccgctggtca tccgaaacga acggtatcgg aaactagttc
     6121 ctgtccaggt ccggcaactc gctccgttct ttaatgatga gcgacaaccg tcagccggca
     6181 tgggcaccgg agcaaatctg ctcgtatcca tcgacatcgc ctctgcttca gatcaggggc
     6241 tgctgctcac cacgactctt gtctctgttg agaccgcaca agtgctggct cagtttgtcc
     6301 tcgaatgtcc gatcaacgcc gtcgcgccaa cggccgaaac tctggcggca gttgtcaccg
     6361 atctcgtcct gccccatggt cccactctca cacggggatg gttcgtcaca ccatgacacg
     6421 atatggcaca ttcctcatct gcttcctcgc caccagtgcg gtcggctgtt cgtctctcat
     6481 actctccaac accccggtgc agggagaaaa tacctcgact cgagaaatcg cgcgcactcc
     6541 gatcgatgta ccagaggtta cccttacacc ccatcacgat cagcaacgcc tcaccattcg
     6601 cgtgacacaa cgctcccaaa tcaccctcga gacggacacg ccgatgcagg tcgaggcacg
     6661 acgctatgtg ttttcgccta ccgctcccct tgccggcgct gttcattgtc cggtactgac
     6721 tgttgcctac gtgatttcag ccggacacct aggctcacaa tctcttgctc ccgcctgccg
     6781 acggctcctg atgcaggagc ctcttgccgg atcaccgcgg gttgccgccg agatcgaaca
     6841 ccgctacgag acacgcacca caacagagcc cgtgcgctac gccacactga gagtggcatg
     6901 ggacgatgcg atacatccgc cactggtcta tgacgtgaat cccgatggag aggtgtccat
     6961 tcctcaacac gcactccgtt cggggacgaa gcgcgacccg tcgacgcttc ccacactgtc
     7021 tatcctcgtc gacaatcgcg tcctctggac tcatcgcctt acccctagcg agatgacacc
     7081 aagtggccca ttgcccgacg aaccgcacac ggcaggtacg agctggccac cgatcgtcgc
     7141 actcgtcctc gaaccgctcg atggcgctgc accacctgcg ttgagagcat ggggacgttt
     7201 ggtgacacag gctttattgg aacgccacat ctgtgtggtc gcaccaataa ccttcgcgaa
     7261 agacttacag gatgagctgc tactccacca atccgggcgg atcagtgacc agacggctgt
     7321 tcactttggc aaatggatac ccgccactgt ggcgctcacc atcagaaacg gggccgccaa
     7381 tcaggggata gctattctag aatttgtgga tattcagagg gcggaacgaa tcagatacga
     7441 cgtcctacgg tatagtagcg actggaaaga ggatgcgcag atgctgaagg cctatatgca
     7501 aacggtcttc cccaaaggat atcaatcctg ctcatagcaa tctcacttcg catcctgtac
     7561 ctcaagcacg caatcaccat acacagtgat cagaagtgaa cctggataca tcgtgatggc
     7621 ttgtttatcg gatccactct tcgacatatc cccaacagat gtctcaatcc tagtgtttaa
     7681 gagcgcacgt ccttttgtca tatcgcgaca atagatcaca agttcatcca acaccgctaa
     7741 ggcgttgccc tcgaaggatg gcctaaaacg ttttgaaaga acggcgatcg ttgaatggcc
     7801 tgggaccttg ccaggaattg ctgatcccac aaatgcacct agccagctat ctgttctagt
     7861 ttctgctaaa gaatggtgcg ctgagcacaa aaatagaaga cttaggctta gaacaatacg
     7921 cgctcgaagc agccgcattg cggcctccct agatatggct ggtaatcgta gtcagttact
     7981 tgttgctgcc ggtattcaca cctggtgaag acggttttga aatcttcaac agcgactctt
     8041 ggccttcctg ccacctcggc atggtaccaa acgcactcga cgcttcttct cccatcatgc
     8101 caagggccga tgtgcgcacc gcattataag cggcattggc agcaaagttc tgtaattctt
     8161 cgggagacat gctttgtgca cgcccggtct tttctaaatg ttcgacagaa ctgcgcgcaa
     8221 acgagatagc attatctatc actccggaat attctttcca ccaggcgccc atattttgat
     8281 gcgagtccaa aagaccgctc ggtcccccaa ctccaccagg gccattcggc acgggtccac
     8341 cacctggctg agccggtgtt cctccaaccc ctcccccacc aaatttcccc attcgtcctt
     8401 tgaggatcaa atatcgcgaa gccccatcca ttccgatccg accaaattca agcccataag
     8461 caatcgcctc gtccgtctgc gtagggtttg taatatcggt gcctttggcc gcaaacgcca
     8521 tttgtgtaat cgtcagattc gccccactgt catgatgaaa ttgcacacga ttgaagtcat
     8581 gaaaatcgga gcctttgtga ccggacgaac tcagcacatt cccgctaccg tcaagtacat
     8641 cgttaaccgc aaatccatgt gccgcatact cattcttcga tagctccttc gtatttgcaa
     8701 tcacgctcgc cccatcgggc gactgtgaat actccactac ctggaagtgt tcttgccctt
     8761 gtgcatcagt acggtactgg ccgattttcc ctgtttgcaa gtccgttact tctgacgcaa
     8821 ttaactggcc agactgagga ctatagtaga acgtcccctg cagctccttg gtctgcccgg
     8881 gatgcatagg atcaggcacg gtgaaaatgc cggatttacg ctcattgtta tagtactgtg
     8941 agttcaatcc gcccaacacc gaatgataca ggacttcgcg ctggccgcca tcatccttca
     9001 taggaccaag gacttgtgtg tctatcgctc cgtttttcgc cgccacgacc ttcaccgtct
     9061 gatcgataga gccgctcata gccccgctgt ttggatcctc cagtgacctg gcaattcccc
     9121 acacctcgtt gccttctcca tctttcataa aagtggaata ggtgttgtcg gtcacattgg
     9181 taatcgtccc gcccagtagc ttgcgttcgc cagtctcact actggtcggg ccgtagtaga
     9241 gattggcgta tacatccata tcgcgatgcc cgtcattgat ccgacgccag cctgactctt
     9301 tatcgatcga ctgtacctcg ttgttgtacc cacgcaggga ctgctcgaac tcgacggtca
     9361 tttggcgcga tcctctcatc tgatcgctat acacactcac cccgtccagg tttgtggatg
     9421 atcttttgaa ggcgtccccc tgaggattaa cggttactcc ctgaaaattt gatagcgagt
     9481 gagttcctcc aggagcagta tatgtctcct gagcgccgat agattgaaga tctccagaat
     9541 cagttcgttc aataacatct cggaactcgt tccctgaggc tcctttaaac gagacgagac
     9601 taccgttatc aatcagccct gtgacatcgc gggccggcgc ccccggttgc tgcgcatacc
     9661 catgaaacat ctcaacttga tgtccatctt gtcccatcac atttaactgc gtgaactgcc
     9721 cagacaaatg agtgccatcc accagattac gttccccgga aatactcttg gtacctccct
     9781 cccgcccgtc aaaaaagctc acctggccgt cgtataacag cgcattggct tgaaacgccg
     9841 g
//
LOCUS       DAOQEW010000143         9724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2235_length_9724_cov_5.618265, whole genome shotgun sequence.
ACCESSION   DAOQEW010000143 DAOQEW010000000
VERSION     DAOQEW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_9724_cov_5.618265"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1549
                     /locus_tag="PLS35_14095"
     CDS             137..1549
                     /locus_tag="PLS35_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19850.1"
                     /translation="MTLLLSGLYFVQYHPPQYGAEAPTLPGALYATLQFIALSVGPAA
                     RMSWVLSIAAVMVCLVPALVIGARHFVARQDRNPFRPVGVVVCAANLLLSALAIGWGR
                     AQVLSLWGGLWPIRYSLFAVPILCLAYFVYELYGSTWTRQAFQTLLFVGMVLLVPANA
                     TQGARWHDWYLQGAGAFERDVQSGASVSRVGERNREYLYRWMDPSFDKVRMLRDSGIG
                     PFAKMAEEPSAPVQAPRHQGMPESPVAESLPAARRELRFVMPEAAEVFLVWGINGWQA
                     APESFRPAGTVVKDHVLHTPMHRQDGVFVMPLALPRGASLDYCFLITKKREAFDITWP
                     LCEGNYHEEFMADGVREIRSRTSLALVRQEIRYHAPEAKEVHLVWGVKGWHVTPQSLW
                     PSGTAIIDHVMHTPMALREGIFVATVTVPVDTAVDYGFQITKRRGIFDLVYPLFDGNY
                     SVRPSQDGQIDMQAKPGLIP"
     gene            1637..5347
                     /locus_tag="PLS35_14100"
     CDS             1637..5347
                     /locus_tag="PLS35_14100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI19851.1"
                     /translation="MNDSPIAEPSAKTMVFIVPSLSRSSAQDEDVVQLLLCAAARVEQ
                     LLSASPGLVVKATILSVDQDLSGWVQVGALFSDRISKHVTVEPLKEPATPCLAIPAHK
                     SVYHVHEFLRERPFDEVHCLDRHGYVYYSAHAKRLGLFFLTTLFCVHVVGGTIFRREC
                     QDLLLDDPDILAIDLLERGTVERADLHYIHDIKAWEWYDEKVVLHAGSTSYDVTWPVD
                     PPTSVVVEGNESSELPALIYCGPLGADGGLPLFCDVVDRLVSQGERLKHVTFVGAAHA
                     IGGVDAVTYIRLRSSGWHLPITIKRDLSLADEMTFLAGLQGVVVCHTARRNHLRSRLI
                     VGGLQNVLHIRAVNESRDGARSGGVLPGQITQTVTRLLRAPLQQSPVSLPPLTQLWKA
                     GRKLPDHEGVRSSLPLGVPNEPQPRVSVCITHFSRPQKLRMALQSLKQQTYRNFDVIV
                     VDDGSPDPDVQKELALIGDEIRPLGWRILFQENRYLGAARNVGARHATGEFLLFMDDD
                     NVAKPHELSTFVAMAQRTGADIITSFFDVFETEATLESDGPPPLRFTPYGADPALGIF
                     TNCYGDANALYSRRAFDKLGGFTEDFGVTHEDWELFCRASLEGVTLLCIPEPLFWYRM
                     DQSAMFRGSRTQLHKSTNLRRHIRPYLEKLPRYQSNLVQLLQGLSTTLPLTTVGTVSR
                     NAAPLALRQPDATLPYGRVAVITRTKDRPLLLHRAIRSVLSQTFKDWVLVIVNDGGDP
                     QRLEKVVDEVREELADRILVVHHPMPLGMQAASNSGIAHCDSDFVIIHDDDDSWDPTF
                     LARTVSYLDDHGWNARLGGVITWSWVIIEEVADDGAVKVHNRFVFNDKLHNVALIDMT
                     VENRFPPISFLFRRAAMDHVGPFRSQHGPLGDWEFHLRLLQHFDVDVIPDPLANYHHR
                     TNTTIGIYGNSVHAHKDQHQASRIHLLNEALRRDLDSGQGLSLAQLLTLGELHHSSKA
                     EQAREFQRLHDYLWNIDQRVQAIATQVHSSKHGLRAKSRRNLVSNGDFRQWPGLGPNQ
                     FGPHETYTFLDVSPGCVLCYDGRQVSYRLEQRKWTTDGQQLAFGKTFLHIENDGYTKG
                     GSWFAIEFMIPSALLLSGHTITISGVSRLNALQGWIGLGGRYLFGDGRQLDWPDHRID
                     VASDFARWTCTVMCPAIQETEVGHGHQSRILLKLPHNQPFDFDLTNLQVELGSESSDF
                     EYSSGKVSLRHRVHLVLRGLKRRLRAAKRAIG"
     gene            5388..6143
                     /locus_tag="PLS35_14105"
     CDS             5388..6143
                     /locus_tag="PLS35_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI19852.1"
                     /translation="MSGISWNLIRHLVARDFRIRYSGSVLGVVWSVLIPMAQLGVLVF
                     TFGSVVPVNIDDYPAFVYSALLPWTWFSACLGSTGSLFFSHRDLVRRPNFQPIILTVV
                     NTLSNLVTFLLSLPLLFLLLAWYQRSVAASLVALPALLVLQAVMTVGLSLLVSTWNVF
                     YRDVSQLVNIVLSLLFFLTPIFYRPVVESKYAMIFQFNPLAVLISSYRNILFEGRLPE
                     WDAWAHITAVSAALLVCGYLVYRHELPDVVDTV"
     gene            6153..7409
                     /locus_tag="PLS35_14110"
     CDS             6153..7409
                     /locus_tag="PLS35_14110"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19853.1"
                     /translation="MHAAMTVTDLSKRYRISRHKVTTLRETVDRWIKGRQDVTRTMWA
                     LRDVSFTIEQGRVFGIIGHNGAGKSTLLRLLCGLGRPTSGRIEAGGYVSGILELGGGF
                     HAELTGRQNLMTVGILNGLTPAEVKAREQDIIAFSELEDFIDQAVRTYSNGMYLRLAF
                     AAAIEFDPAILIIDEVLSVGDERFQQKCLDRIAAFRASGKTLVITSHDTDQIQRLCDE
                     VLVLEEGRAIMQGDPKSAIACYHDLMRQRTERRAAHIGGGSTRSLTTASGTRQGTQEA
                     TIAVVRLCRSDGQAVETIRSGEGILAELEIDAPPSIEDFALTVGVFNEGHVKCFEATI
                     ASVQGTLGKVSPHSSIQCHLKHLPLLSGSYFINVGIYPTDWSYVYDFHWQMHPVTVFD
                     ERTSNSQAGGIVAVETHWTIISRGDV"
     gene            7406..9313
                     /locus_tag="PLS35_14115"
     CDS             7406..9313
                     /locus_tag="PLS35_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19854.1"
                     /translation="MRPAIRAAGLVCCCVIMPLWHVVQVGAETTTAPASPPRMLTREI
                     RYHMPEAGEVWLVWGVNGWHRLPEAAWPPGTVLGKTGLMTTRMIEQGDAFVATVAVPN
                     GTALDYCFLLTKKREAFDITWPLCEGNYSEVATRSGVTEIGSRLSLAVVTQEFHYHMP
                     DAQEVSMVWGVNGWSPVPESLRPLNTKMSDNLMVSPMRKREQEFIVSVQVPVGATIDF
                     GFRITRSAMGSVNIWEGGGAEGFHEPVRGGSSIRVKSNAIILPRGRPSRDEDNGWHVA
                     WAIVGAVSAPLLLAGLLVMLRRRQARQRSANPFNPRREKPSSALDGLLILAAVGIGAF
                     GAEQMLRNVYPHEGFGIAAEDEWFRQAPYSANQLFTIDPTLGIRPWLRTGRYTEYGTV
                     PNMYRLAKDSRHTRILFVGNTVTYEGTLLHALRELHGEDRFEYWNAGVPTFNTVQQIA
                     YYHAYNARTAPDHVILTIQPDDLGTIPIVFRNHTAELEVHRPAIPRKDLDTELYGSSY
                     LYRYVKGLLISPEEKLHRLWQEYRDSVKSFSERLAHDHIRLSVVVLPVLASQQEWTQA
                     ERDRRQALFSVLTELQIEHYDLAQAFEPGRAENAVLPPMSKESWAPSSEMVSAFAQYM
                     THEGLFRTKEH"
     gene            9315..>9724
                     /locus_tag="PLS35_14120"
     CDS             9315..>9724
                     /locus_tag="PLS35_14120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNI19855.1"
                     /translation="MSPHHTHRHENRARSNHADESTHDVQRGDSSESISGLVSIVIPC
                     YQQAHFLPDAISSILRQTYPDIEIIVVDDGSTDHTRDVALSHPGIRYIAQKNAGVSSA
                     RNTGFRMSRGEYIVFLDADDRLIADAIEIGTRSLA"
BASE COUNT         1979 a         2716 c         2869 g         2160 t
ORIGIN      
        1 ggaggctgct ggcaacgaac accagggacg ctccgatgag aacccgcccg atgatcttgt
       61 gatcgccgga gccgagaacc tgctcgaccg gcgtgggctg cgtgcgcggc gaagctgact
      121 ggtcggcgcc gctgctgtga cgctactcct gtccggactc tatttcgtgc agtatcaccc
      181 gccacagtat ggcgccgaag cgccgaccct gccgggcgcg ctctacgcaa cgctgcaatt
      241 tatcgcctta agtgtcggac ccgccgcaag aatgtcctgg gtactctcga ttgctgcggt
      301 gatggtgtgt cttgtgcctg cccttgtcat tggcgcgagg cacttcgtcg cacggcagga
      361 caggaaccca tttcggcctg tgggagtggt cgtctgcgcg gccaatctcc tgctgtccgc
      421 cttggctatt ggctggggcc gcgcgcaagt tctttcattg tggggagggc tctggcccat
      481 ccgctattcc ctgttcgccg ttcccattct gtgtttggcg tactttgtct acgagctcta
      541 tggatccact tggacgcgtc aggcgtttca aacgttgtta ttcgtcggca tggtccttct
      601 cgtaccggcg aatgcgacgc aaggtgcacg atggcacgac tggtatctgc agggtgccgg
      661 ggcattcgag cgagatgtgc aatcgggtgc atcggttagc cgggtaggcg aaaggaatcg
      721 tgaatactta tatcgatgga tggaccccag tttcgacaaa gtgaggatgc ttcgagacag
      781 tgggatcggg ccatttgcga aaatggccga ggagccgtct gctccagtcc aagcgcctcg
      841 tcaccaggga atgcccgagt ctcccgtagc agagtctctg cctgcggcga ggagagagtt
      901 gcgctttgtt atgcccgaag cggccgaggt gtttctcgtg tggggcatca acggctggca
      961 agccgcccct gaatcgtttc gtcccgccgg aaccgtggtg aaagaccacg tgttgcacac
     1021 gcccatgcat cgacaagacg gagtctttgt tatgccgctg gcccttcctc gaggcgcgtc
     1081 gctcgactat tgcttcctca tcacgaagaa gcgggaggcg ttcgatatta cctggcctct
     1141 gtgtgaaggg aattaccatg aggagttcat ggcagacgga gtgagggaga tccgttcacg
     1201 cacgagcctt gcgctggtga gacaggaaat tcgctaccac gcgccggagg ccaaagaagt
     1261 gcatctggtg tggggcgtga aagggtggca tgtcactccc caatcactct ggccttccgg
     1321 cacggccatt atcgatcacg tcatgcacac acctatggct ctacgagagg ggatattcgt
     1381 cgccaccgtg actgttccgg tcgataccgc ggtcgattac gggtttcaga ttacaaaacg
     1441 tcgaggcatt tttgatttgg tgtaccccct gttcgatgga aattacagcg tgcgcccgag
     1501 tcaagacgga cagatcgaca tgcaggcaaa gcccggactc ataccgtgat ggatccggag
     1561 gcatgggtag ggaagaggtt gcgagtcgca gtatttatgc aggaacgaga cgatagatgg
     1621 aggccagctt ccctatatga atgactcacc catcgccgaa ccatcggcca aaacgatggt
     1681 gttcatcgtg ccgtcgctca gccgctcatc cgcgcaggat gaagacgtgg tgcaactcct
     1741 gctatgtgcc gcggcgagag tggaacaact gctatccgct tcacctggct tggttgtgaa
     1801 ggcgacaatc ctgtccgttg atcaagacct cagcgggtgg gtacaggttg gagcgctgtt
     1861 ctctgacaga atttcgaagc acgtcacggt tgaacccctg aaggagccag caacgccctg
     1921 cctcgccatt ccagcgcata agtccgtcta tcacgtccat gagttcctga gggagcgccc
     1981 cttcgatgag gtgcactgcc tggaccgcca cggctacgta tactactcgg cgcacgcgaa
     2041 gaggcttggc ctgttcttcc tgacgacgct tttttgtgtt cacgttgtgg gggggacgat
     2101 cttccgccgc gagtgtcagg atctgctcct cgacgatcct gatatcctgg cgattgatct
     2161 cctggagcgt ggcacggttg aacgggccga cctccactac attcacgata tcaaagcgtg
     2221 ggagtggtat gacgagaagg ttgtcctgca cgccggcagc acgtcttatg atgtcacatg
     2281 gcctgtggat ccgcccacat cggtggtcgt ggaaggcaac gagtcttcgg aattaccagc
     2341 actgatctat tgtggaccac ttggcgccga cggagggttg ccgctctttt gcgatgtcgt
     2401 ggaccgtctc gtgtctcagg gggaacgcct gaagcatgtg acgtttgtcg gcgcggctca
     2461 cgcgatcggc ggggttgatg cggtcacgta catccggcta cggagttccg gctggcatct
     2521 gccgattacg atcaaacggg atctttcact cgctgatgaa atgaccttcc tggccggcct
     2581 tcagggagtc gtcgtttgtc atacggctcg acgtaaccac cttcgttcac gtctgatcgt
     2641 gggagggctg cagaatgttc tgcatatcag agcagtaaac gaatctcgtg atggagcgcg
     2701 cagcggaggt gtcctccccg ggcagataac ccagacggtc actcggctcc tacgtgcgcc
     2761 cctccagcag tctcctgtct cgctccctcc gctgacgcaa ttgtggaaag cggggcgcaa
     2821 gcttcccgac catgaaggcg tcaggtcctc tctcccattg ggtgtaccga atgagcctca
     2881 gcccagggtc agcgtctgca tcacgcattt ctcgcggccg cagaagttac gcatggcgct
     2941 ccaatcgctc aaacagcaga cctatcgcaa tttcgacgtg atcgtggtgg acgacggcag
     3001 cccggatcct gacgtccaaa aggaactcgc actaattgga gatgagatcc ggccattggg
     3061 ctggcgcatt ctcttccaag agaaccggta tctcggtgcg gcgagaaacg ttggggcacg
     3121 gcacgcgact ggagagttct tgctgttcat ggacgacgac aacgtcgcaa agccccatga
     3181 actgagcacg tttgtggcaa tggcgcagag gactggagcc gacattatta cgtcgttttt
     3241 tgacgtattt gaaactgagg caacgctgga atcagatggg ccgccgcctc tccggttcac
     3301 tccatacgga gccgatccgg cattgggtat cttcacgaac tgctacggtg atgccaatgc
     3361 attgtattcg cgccgcgcgt tcgacaagtt aggaggcttt accgaagatt tcggcgtcac
     3421 gcacgaggac tgggagttat tctgccgtgc ttcgctcgaa ggggtcacac tgctctgcat
     3481 tccggaaccg ttgttctggt accgcatgga ccagagcgcg atgttccggg gatcccgtac
     3541 acaactgcat aaaagcacca atcttcgccg gcatatccgg ccctacctgg aaaagctgcc
     3601 acgatatcag agcaatctgg tgcaactgct gcagggactt tccaccacat tgcctctaac
     3661 caccgtcggc acggtctccc gcaatgccgc gccactggct ctccgccagc ccgatgccac
     3721 gcttccgtat ggccgggtcg ctgtgatcac tcgaacgaaa gatcgccccc ttctgctgca
     3781 ccgggccatt cgcagcgtgc tgtcacaaac attcaaggat tgggtgctgg tgatcgtcaa
     3841 tgacggcggc gacccacagc ggcttgagaa ggttgtggac gaggtgcgag aggagttagc
     3901 agaccgcatc ttggtcgttc atcatccgat gccgctcggc atgcaagcgg catccaattc
     3961 cggcattgcc cattgcgaca gtgacttcgt catcatccac gacgacgacg attcatggga
     4021 ccccacgttt ctcgctcgca ccgtctctta tctggatgat catgggtgga atgctcggct
     4081 aggcggggtg atcacctggt catgggtgat catcgaagaa gtggccgacg atggtgcagt
     4141 gaaagttcac aatcgattcg ttttcaatga caagctgcac aatgtcgccc tcatcgacat
     4201 gaccgtggaa aaccggtttc ctcctatctc attcctcttt cgtcgagctg cgatggatca
     4261 cgtcggtcca tttcgatccc agcatgggcc gctgggcgat tgggagttcc accttcggct
     4321 cctgcagcac ttcgatgtcg atgtgatccc tgatccactg gcgaattacc atcatcgcac
     4381 caacaccacg atcggaatct atggaaactc cgtccatgca cataaagatc aacatcaggc
     4441 atcgcgaatt catctgttga atgaggcgct gaggcgtgat ctggattccg ggcaggggtt
     4501 gtccctggcg caactcctga ccctcggcga actgcatcac agctcaaaag ctgagcaggc
     4561 cagagaattt cagcggctgc acgattacct gtggaacatt gaccagcgcg tgcaagctat
     4621 cgccacgcag gtgcactcat ccaaacatgg gctccgtgcg aaaagcagac gcaatctggt
     4681 gtcgaacggg gatttccggc agtggccagg gctgggtccc aaccagtttg gtcctcacga
     4741 gacgtatacg tttcttgatg tcagtcccgg gtgtgttctg tgttacgacg ggcgccaggt
     4801 gtcgtatcgt ttggagcaac gcaagtggac gactgacggg cagcagttgg cgttcggcaa
     4861 aacgtttctg catatcgaaa acgatgggta cacgaagggt ggatcatggt tcgcgatcga
     4921 attcatgatt ccttcggcat tactcctttc cggccacacg attaccatct cgggagtctc
     4981 gcgcctcaat gccctacagg gctggatcgg cctcgggggc cggtatctgt ttggcgacgg
     5041 ccgtcagctt gactggcccg atcatcgcat cgacgtcgcg tcggactttg cccgatggac
     5101 ctgtaccgtg atgtgtccgg ccattcagga gaccgaggtg gggcacgggc atcagtcacg
     5161 gattctcctc aagctgcctc acaatcaacc cttcgatttc gatctgacca atctacaagt
     5221 tgagctgggc agcgaatcgt ctgactttga atacagtagc gggaaggtgt ccctacggca
     5281 cagggttcat ctggtgctgc gagggttgaa gcgaaggctg cgggcggcga aacgcgcgat
     5341 aggatgagtt cgaactgcgt cgtgaacaga catcgtcgga ggtacacgtg tcgggcatct
     5401 cgtggaattt gattcgtcat ctggtggcca gggacttccg catccggtac tccggatcag
     5461 tcttgggtgt cgtctggtca gtgctgatac ccatggccca actcggggtc ctggtcttca
     5521 cgtttggcag cgtggttcca gtcaacatcg acgactatcc cgcgttcgtg tacagcgccc
     5581 tcctgccgtg gacatggttc agcgcgtgtc tcgggtcaac cggatcgctc ttctttagtc
     5641 atcgcgacct cgtgcggaga ccgaattttc agcccatcat cttaaccgtc gtgaatacgt
     5701 tgtcgaatct cgtcaccttt ctgttgagcc tcccactcct gtttcttctg ctcgcgtggt
     5761 atcagcggag cgtggcggcg agtcttgtcg cactaccggc actgctggtc ctgcaggcgg
     5821 tcatgacggt gggattgagt ctcctggtgt caacgtggaa tgtgttttac cgggatgtct
     5881 cccaacttgt gaatattgtg ctctcgctct tgtttttcct gacgccgatt ttttatcgcc
     5941 cggtagtgga atccaagtac gccatgatct ttcagttcaa tcctctggcg gtgctcattt
     6001 cctcctatcg gaacatcctt tttgaaggac gtctgccgga gtgggatgcc tgggcgcaca
     6061 tcacggcagt gagtgccgcg ctgctggtct gcgggtatct cgtgtatcgc catgaattgc
     6121 cggacgtcgt ggacactgtc taggagagac cgatgcacgc tgccatgacg gtcaccgact
     6181 tgtcgaagcg ctaccgtatc tcacggcaca aggtgaccac gcttcgcgaa acggtcgatc
     6241 gctggatcaa gggccgtcag gatgtgacgc ggacgatgtg ggcgttgcgc gatgtctcct
     6301 tcaccatcga acagggacgg gtcttcggca tcatcggcca taacggagcg ggaaaaagca
     6361 cccttctccg actgctctgc ggcctgggcc ggccgacgtc aggccgtatc gaagccgggg
     6421 gatatgtgag cggcattctg gaactgggcg gcgggtttca tgccgagctg acgggacgac
     6481 aaaatctcat gacggtgggc atcctcaatg ggctgacgcc agccgaggtg aaagccaggg
     6541 agcaggacat catcgccttt tctgaactgg aagactttat cgatcaggcg gttcgcacct
     6601 attcgaacgg catgtacctt cgtctcgcat tcgccgcagc catcgagttt gatcctgcga
     6661 ttctgatcat tgatgaagtg ttatccgtcg gtgatgagcg gtttcagcaa aaatgtctcg
     6721 atcgcattgc ggccttccgg gcatccggca agacgctggt cattacgtcg cacgatacgg
     6781 atcagattca gagactgtgc gatgaagtgc tcgtcctcga agagggccgc gccatcatgc
     6841 agggcgaccc caagagcgcg atcgcttgct accacgacct gatgcgtcaa cgtacagaac
     6901 gccgggctgc tcacattggt ggcggctcta cgcgatcact gacgactgca agcgggacga
     6961 ggcagggcac gcaagaagcc accatcgccg tggtccggtt gtgccgcagc gacggtcagg
     7021 ccgtggagac tatacgtagc ggcgaaggga tcctggcgga attggagatt gatgcgccac
     7081 cctcaatcga ggacttcgcg ctcaccgtcg gggtgttcaa tgaggggcat gtcaaatgtt
     7141 ttgaagccac catcgcatca gtacaaggga cattgggcaa agtgagccct cacagttcca
     7201 tccaatgtca tctcaaacac ctgccactgt tgtccgggtc gtatttcatc aacgtcggca
     7261 tttacccgac ggattggtcc tatgtctacg attttcactg gcaaatgcac ccggtgacgg
     7321 tatttgacga gcgaacgagc aactcgcagg cgggaggaat tgtcgctgtt gaaacacatt
     7381 ggacgatcat ctctcgcggt gatgtatgag accggctatt cgcgcggcag ggttggtctg
     7441 ctgctgcgtg atcatgcccc tgtggcacgt ggttcaggtg ggcgccgaga ccacgaccgc
     7501 accggcgagc ccgcctcgca tgttgaccag ggaaattcgt tatcacatgc ccgaagcagg
     7561 ggaagtgtgg ctggtgtggg gcgtgaacgg gtggcatagg ctccccgaag cggcctggcc
     7621 gccgggaacg gtgttgggga aaaccggact catgaccact cggatgatcg aacaagggga
     7681 cgcgttcgtg gccactgtcg cggttccgaa cggtacggca ttggactatt gcttcctgct
     7741 caccaaaaag cgcgaggcgt ttgatatcac ctggccgctc tgcgaaggaa attacagcga
     7801 ggtcgcgacg cgttccggtg tgacggaaat cggatcgcga ctgtcgttgg ctgtggtcac
     7861 acaggagttt cattaccaca tgcccgatgc gcaagaggtg tcgatggtat ggggcgtgaa
     7921 tggatggtct cctgttccgg agtcactgcg tccgctgaac acaaagatgt ctgacaatct
     7981 catggtgtca cccatgagaa agagagagca ggagttcatc gtctcggtgc aggtgcccgt
     8041 aggggctacg atcgacttcg ggtttcgaat caccaggtcc gccatggggt ccgtcaatat
     8101 ttgggagggc ggaggcgccg aaggatttca tgaacctgtg cgaggtggtt catcgatccg
     8161 ggtgaaaagc aatgcgatca tccttccacg cggacgtccc tctcgtgacg aagacaatgg
     8221 atggcatgta gcatgggcaa ttgtcggcgc agtgtccgcc cctctcctat tggcgggact
     8281 gctggtgatg ttgagacggc gccaagcgcg gcagcggagc gcgaatccct tcaaccctcg
     8341 ccgggagaag ccaagctcag cactggacgg ccttctgatt cttgccgctg tgggcatcgg
     8401 cgccttcggt gccgagcaaa tgcttcgaaa cgtatatccc cacgaaggat tcgggattgc
     8461 ggcggaagac gagtggttcc ggcaagcccc ctatagtgcc aaccaattgt tcacaatcga
     8521 tcccacactc ggcatacggc catggctgcg gacgggcagg tatacggagt acggaaccgt
     8581 gcccaatatg tatcgcctgg ccaaagattc gcgacatacc cggatcctgt ttgtcggcaa
     8641 caccgtgacg tatgagggca cattgcttca cgccttaagg gaactccacg gagaagaccg
     8701 gttcgaatat tggaatgccg gagtgccgac gttcaacacg gttcagcaga ttgcctacta
     8761 tcatgcgtac aacgcgcgta ccgcgcctga tcatgtcatc ttgaccattc aaccggatga
     8821 cctcgggacc atcccgattg tctttaggaa tcacacggcg gaacttgagg tgcaccgtcc
     8881 tgcgatcccc cggaaagacc ttgatacgga gctctacgga tcgtcttatc tgtatcggta
     8941 tgtgaaggga ctcctgatct cacctgagga gaagctgcat cgcctgtggc aggagtaccg
     9001 cgacagcgta aagagcttca gcgagaggct cgctcacgac cacattcgct tgagtgtcgt
     9061 agtgcttccg gtcctcgcct ctcagcaaga gtggactcag gctgagaggg accgacgaca
     9121 agcgctgttc tcagtgctca cggagttaca gattgaacac tacgatcttg ctcaggcctt
     9181 tgaaccgggc cgagcggaga acgcagtact tcctccgatg agcaaggaaa gctgggcgcc
     9241 ttcatctgaa atggtctccg cctttgctca atatatgact cacgaaggac tctttcgcac
     9301 caaggaacac tagcatgagt cctcatcata cgcatcgcca tgagaatcgg gccagatcca
     9361 atcacgcgga tgagagcacg catgatgtcc aaaggggtga ttcgtcagaa tccatctccg
     9421 gcctagtctc catcgtgatt ccctgctatc agcaggcaca ctttctcccg gacgcgatat
     9481 ccagcatcct tcgccaaacg tatcccgaca ttgagatcat cgtggtcgat gacgggtcga
     9541 cggatcatac gagagacgtg gcgctgtcac atcccggaat ccggtatatc gctcaaaaga
     9601 acgcgggggt gtccagcgcg agaaataccg gattccgaat gagtcgtggt gagtacattg
     9661 tctttctcga tgccgatgac cgactcatag cggacgctat cgaaatcggc acacgcagtc
     9721 tcgc
//
LOCUS       DAOQEW010000144         9616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2275_length_9616_cov_6.289300, whole genome shotgun sequence.
ACCESSION   DAOQEW010000144 DAOQEW010000000
VERSION     DAOQEW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9616
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_9616_cov_6.289300"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..569)
                     /locus_tag="PLS35_14125"
     CDS             complement(102..569)
                     /locus_tag="PLS35_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNI19856.1"
                     /translation="MRLFATDVDGVLTDAGMYYSESGDEWKKFNTRDGMGIKLLQKAG
                     IVTAIITQESTKIVMRRAQKLTIPEVHQGAYDKLAVLKDLIARHGLTMKQVAYIGDDV
                     NDLQALGAVGFSASPADGMPPVLKVVRYVCKKNGGQGAVRELADLILAAQKSQ"
     gene            complement(728..2623)
                     /locus_tag="PLS35_14130"
     CDS             complement(728..2623)
                     /locus_tag="PLS35_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNI19857.1"
                     /translation="MKRIAVQVFQKVSSRFGQTILAYRTALIICVQAGLILAANFTAF
                     AFRFDGQIPALYKDTMWRGMPLVCVVYLLGLWGFGIHRGLWRYVGLHDLGRIMWACVV
                     STIVTFVLLHPILGRVEYPRSVILGTGLLAGLNIAGIRLLVRWFREWLQIVGPTARRV
                     LIVGAGNAGELLVRNMQMNPAYNYRPVAFVDDDPVKQGAKIHGVPIAGCIQNIREIVR
                     RFEVHEILMAIPSAPLALKQKTLAAVEGAKVPIKILPSVRQLLEDPALLQQVRPMSLE
                     DLLQREPIQTDRQELHPLIEGKTALVTGAGGSIGSELCRQIARYRPATLVLFERYENA
                     LHALLLELQAAFPDVCILPIVADVTMPERVAEVFRQTQPDIVFHAAAHKHVPLMELNP
                     KEAVRNNVWGTRVVAEAALASRVDRFVLISTDKAVNPSSVMGATKRIAEHVIQGLSQL
                     GQTRFTVVRFGNVLGSNGSVVPLFAEQIRKGGPVTVTDREIKRFFMTIPEAVQLVLQA
                     SVMGQGGEVFVLDMGEQIKVADLARNMIVLSGFVPDKDIEIVYTGLRVGEKLYEELFE
                     AGEQVLATAHGKINRAVGAPLVAEGFERWIAELHSKLAESDEEELLHDLKRIVPTFQP
                     TSPLRVS"
     gene            complement(2684..4006)
                     /locus_tag="PLS35_14135"
     CDS             complement(2684..4006)
                     /locus_tag="PLS35_14135"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI19858.1"
                     /translation="MKISRIPAVWASMEANHGPTLDLIVRALLYVYIFSLPFLPLLVV
                     ERNIFLVLLVLLPLWCLLRRRHFFVSSPVDLPLIAFVAWVAFTIPFSAHPAYSMKEFG
                     KLLQQVVIFYAVLYFFRERTARDLLTYLLLGCVLVVSAYGITQFQWYDGQATRSFFPA
                     EVWLTTYLVLLGPLFFSLAYYEVTNRLRYVYGCGSILVTICLLMTRSRAGLVALFVEL
                     WVMAWLLKRRSAVTVAGGATVVCLLAALFLIRVDTGSDGQLRLTLRDGTPVRTDGASV
                     IHRFDIWAFALSKIQEHWFVGIGYGKDNYKLVYGDEPEEVQPGHHPVKTAGAHNIVLA
                     SALHVGLIGMVLFLWLWGKLVRRLIQECHRTEGSTARAIVVGATVGVIGLGVRLMFDQ
                     MLVGTLAVQFWVVVGIAVLHVNSQRESDREGVVAAVSGAATLAGARSG"
     gene            complement(3996..5075)
                     /locus_tag="PLS35_14140"
     CDS             complement(3996..5075)
                     /locus_tag="PLS35_14140"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI19859.1"
                     /translation="MALLGVGGLVLVLTYWGIGWLCMPGSRLYLLDYPNARSLHEIPT
                     PRTGGLALLVSAAIGLIVATLWEFPTQAWWLSASQTSPTAGAWILAMTVLLAVVSFWD
                     DRDGLPVLLRLAVQLIAAATVVIGAGVSLSSLSLPAVGSLSFEGVAVPLTLLFVLWMT
                     NLYNFMDGMDGFAGGMTVFGFGAIAYFAWQAQHPFILILSLIISMGAVGFLLYNLPPA
                     RIFMGDVGSISLGFLSGTLSVLGVQDGLFDIWTPLLVFSPFVLDATTTMLGRAVRGEK
                     IWEAHRTHYYQRLVLHGWGHRKTLLSEYALMALCVAAAVLYHGATDSGRLAILVVWIL
                     GFAGLAVGVGLMEQRAGQQGRMYED"
     gene            complement(5108..6055)
                     /locus_tag="PLS35_14145"
     CDS             complement(5108..6055)
                     /locus_tag="PLS35_14145"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI19860.1"
                     /translation="MNVLVTGASGFVGHAVCAQLVAQGVTVTAAVRRPAAQPVRGLAY
                     CQVRDLGPDTDWHEALTGVHVIVHCAARVHVMQETDADPLPAFRTANVCGTVALARQA
                     AELGVERLIYVSSVKVNGEGGQDAYRETDVPAPQDAYGVSKYEAEVGLREIAAHTALK
                     VVVVRPPLVYGPGVRANFQLLMQALARGIPLPLGAIRNRRSLVGLGNLVDFIVTCIEH
                     PRAANEIFFVSDGEDLSTTELIDRLALAMNRPARLIPVPMPALRSGLTLLGQREMAAR
                     LCGNLRVNITKARTLLGWCPPIRVDEELRRAAEYYLQHQ"
     gene            complement(6052..7173)
                     /locus_tag="PLS35_14150"
     CDS             complement(6052..7173)
                     /locus_tag="PLS35_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI19861.1"
                     /translation="MSRCPKLLFVVTEDWYFVSHRLPLALAAQKAGFDVVVATREGEH
                     GERIRAEGIRLIPCHLSRRRGNPLQEIAMLARLYRRERPDIVHHVALKPVLYGALAAK
                     IAAVPHVVNAVAGLGWLFTSNTLAVKALRPAFCRLLVWLLGASSSCTIVQNPDDFGLL
                     TQKGVPEDRLRLIRGAGVDTEVFAPPSQLPSEPVTVVFAARMLLDKGVREFVEAAHVL
                     TDAGVKARFVLVGDPDPGNPASVPDTSLRAWHGQHGIEWWGRREDMPAVLQLGHIACL
                     PSYREGLPKVLLEAAACGLPLVTTDAPGCRDVVHDGDNGFLVPIRNAQALALALARLI
                     EDAALRATMGRRAREIVLAEFSQERIIKETLAVYLELTP"
     gene            complement(7170..7643)
                     /locus_tag="PLS35_14155"
     CDS             complement(7170..7643)
                     /locus_tag="PLS35_14155"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNI19862.1"
                     /translation="MIFAALLWSIVLLVPQKYGNMSVQSKLSYESSNLERWETLTHGL
                     EMWQQSPVLGVGLGVFNEKSPMWAGHPQVIHSTPLWILAEFGLLGGAVFGWTFFLLAH
                     HAIRSGTTQPANCILLLLLVAFAVFSLAHEIFYQRILWLVLGAVLTRPYAHRRHA"
     gene            complement(7640..9334)
                     /locus_tag="PLS35_14160"
     CDS             complement(7640..9334)
                     /locus_tag="PLS35_14160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNI19863.1"
                     /translation="MLSNFSFRRLPAVFVSIFLIAFVLWSAGVEDVLRRLAGFPPWAV
                     ASMLALLTANLFVVSFRFWRVLAHFGIALPWDIALRASIAGTVAGLFVIPLFGQIMGR
                     QGVLNSFGVQPVVNASLAAYERALLALLSGVLGTLGAVYLLGRSAVSDFFNQIALPEI
                     VVAAIGGWALSLWLGGSRFERKLSRRTLSWTNLEHVMSIAGLTLTGQLLMLACFVVGI
                     LAVSQDIPIASLFAASALISLAASMPITVGGWGVREVAAVYVFGLLGIPAADALAMSV
                     MVGLCSTLVILASSPFCFRNSLMSDLPKPPAVMIHSVSEIEEFTAWVLGTVVAVAVFF
                     QVHVSLSGGPINLNLADPFAILALAAVSLHALFARQFPVWRVPQFNFALCVISLLLLV
                     GFVHGWLEIGITQWALGGRLLGWLVLLGYLSSGYVIVANVGTHGLRRFAETLIATAVV
                     VVMLQVALRLLSHWGVNAAAQLTQNFEGYAGNRNAFALQVLAGMALFLGYSHLYARHN
                     IRVPRSVRPWIFSLLLGILLAGLVWTGSRTAMLVGAMALVLAWLGGMADHKMLGWG"
     gene            complement(9324..>9616)
                     /locus_tag="PLS35_14165"
     CDS             complement(9324..>9616)
                     /locus_tag="PLS35_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNI19864.1"
                     /translation="YRHVPKALIDRPKMGFGVPIDVWLRGPLKGWAEELLDESRLQRE
                     GYFDPQAIRTKWLEHLTGRRNWAYYLWDVLSFQAWLESMGDSAHNGAHILAQ"
BASE COUNT         2471 a         2983 c         2415 g         1747 t
ORIGIN      
        1 aacctggtcc cgctgccgcc cgccagaata accggataga ggtgatttgt catatttttt
       61 tcgactccgc ttccgttcaa catgacgcgg ttgttgttct ctcactggga cttttgggct
      121 gccagtatga gatccgccaa ttcccgcacg gcaccctgcc cgccgttctt cttacatacg
      181 tagcgtacga ccttcaacac gggaggcatg ccatccgctg gcgacgcaga gaacccgacc
      241 gcacccagcg cctgaaggtc gttcacgtca tcaccgatgt aggcaacttg cttcatcgtg
      301 aggccatgcc gcgcgatgag atccttcaac actgcgagct tatcataggc cccctgatgc
      361 acctcgggaa tcgtcaattt ttgcgcccgg cgcatgacga tttttgtcga ttcttgggta
      421 atgatcgccg tgacgattcc ggccttctgc aacagcttga tccccatgcc gtcgcgcgta
      481 ttgaactttt tccattcgtc tccggattct gagtagtaca tgccggcgtc cgtcagtacg
      541 ccatcgacat ccgttgcgaa taaacgaatg ccccggagaa tcccttgaag ggaagcaggt
      601 gacttcgatg gagtgctggt tcgacgtgtt gtgctaggca tgcgaaggct ttcgggggcg
      661 atgttaacaa gccatcacat gaaactcaag aacactcaat ggcatgcgat cgatgcttgc
      721 tgatcaatta agaaactcgt agaggagacg ttggctggaa cgtaggcaca attcttttga
      781 gatcatgcag caattcctcc tcgtcactct ctgcaagttt cgagtgcagt tctgcgatcc
      841 atcgctcgaa cccttccgct accagcggcg ccccaaccgc ccggttaatc ttaccatgag
      901 cggtcgccag gacctgctcc ccagcttcga acaactcttc gtacagtttt tctcccacgc
      961 ggagtcctgt atagacaatt tcgatatctt tatccggaac aaacccggac aacacaatca
     1021 tattcctggc gagatccgcg acttttattt gctcccccat atctaagaca aacacttccc
     1081 ccccctgtcc catgacactc gcctgcagga caagttgaac tgcttcggga atggtcataa
     1141 aaaatcgttt aatttcacga tccgtcaccg tgaccggacc gccctttctg atttgctcgg
     1201 caaacaatgg caccacactg ccgttgctgc ccaatacatt cccaaaccga acaacggtaa
     1261 atcgcgtctg tcccaattgg ctcagccctt gaatcacatg ttctgcgatc cgcttggtgg
     1321 cgcccatcac actcgatgga ttgacagcct tatcggtaga aatcaagacg aagcgatcga
     1381 ccctcgacgc caacgccgcc tccgcgacca cacgagtgcc ccagacattg ttccgcacgg
     1441 cttccttggg attcagttcc atcaacggaa cgtgcttatg cgccgcagca tgaaacacaa
     1501 tgtctggctg agtttgccga aacacctctg ccacacgctc aggcattgtc acgtcagcaa
     1561 cgatcggaag aatacagaca tcgggaaagg ccgcttggag ttccagcaac aatgcatgca
     1621 aggcattttc ataccgttcg aatagcacca aggttgccgg ccggtaccgg gcaatttgtc
     1681 ggcataactc cgatccaatt gacccccccg caccagtgac taaagcggtt tttccctcga
     1741 tcaacgggtg tagttcctgc cgatccgttt gaatcggctc ccgctggaga agatcctcta
     1801 agctcattgg ccgaacctgc tgcaacaggg ctggatcttc cagcagctgt cgcacgctcg
     1861 gaagaatctt gattggcacc ttcgcgcctt cgacggcagc aagcgttttt tgcttcaagg
     1921 ccagcggcgc agaagggatg gccatgagga tttcgtgcac ctcaaaacga cgcacgattt
     1981 ctctgatgtt ctgaatgcaa cccgcaatcg gaaccccatg gattttggct ccttgcttca
     2041 ccggatcgtc atccacaaac gcgaccgggc gatagttgta ggcggggttc atctgcatgt
     2101 tccgaacgag caattcaccg gcgttcccgg cccccacaat taagacgcga cgagccgtag
     2161 gtcccacgat ttgcagccac tctcgaaacc accgtacaag cagtcgaata ccggcaatat
     2221 tgaggccggc cagaagtccg gtcccaagaa tgacagaacg gggatactcc actcggccca
     2281 aaatcggatg caacagaacg aatgtcacaa tcgtgctgac cacacaggcc cacataatcc
     2341 gcccgaggtc atgcaagccg acataccgcc acaaacctcg atggataccg aacccccaca
     2401 gtccgagcag atagacgacg cacacgagag gcatacctct ccacatcgtg tccttataga
     2461 gggcgggaat ctgcccatcg aaacgaaagg caaaggccgt gaaattggcg gccaaaatca
     2521 acccggcctg gacgcagatt atcaatgccg tacgataggc aagaattgtc tggccaaatc
     2581 tcgaggagac tttttgaaac acctggacgg ctatgcgctt catgcctgat tcacacagat
     2641 ttcagcagtc agtgattaac cgatcgatct caatccgctc agattatccc gagcgagcac
     2701 ccgcgagggt tgccgcgcct gaaacagcgg ccactacacc ctccctgtcc gattcgcgtt
     2761 gtgagttaac atgaaggacc gcgatcccaa ccacaaccca aaactgaact gcaagagtac
     2821 cgaccagcat ctgatcaaac atcagccgca cgcctagtcc gatcacaccg accgtcgctc
     2881 ccaccacaat ggctcgagct gtcgagcctt ccgttcggtg acattcctgg atgagtcggc
     2941 gaaccagttt cccccacaac cacagaaaca acaccatacc gatgagcccg acatgcagcg
     3001 ccgacgcgag cacaatgttg tgggcgccgg ccgtcttcac aggatgatgc ccaggttgca
     3061 cctcttctgg ttcatctcca tacaccaact tgtagttgtc ttttccgtag cctatcccca
     3121 caaaccaatg ctcctgaatc tttgacaaag caaacgccca aatatcgaaa cgatgaatca
     3181 cagacgcgcc atcagttcga accggagtgc catcccgtaa agtaagacga agttgaccat
     3241 cagaaccggt atccacgcga attagaaaca gggcagcgag caagcatact accgtcgcac
     3301 cacccgcaac ggtcactgcg ctacgacgct tgagcaacca ggccatgacc cacagttcga
     3361 caaacagcgc caccaaaccc gcacgtgatc gggtcatcag cagacagatc gtgaccaaaa
     3421 tgctcccgca tccatacacg tagcggagac gattcgtgac ttcgtagtag gcaagtgaga
     3481 aaaacagcgg ccccagaagc acaaggtaag ttgtcagcca cacctcggca gggaaaaatg
     3541 accgagtagc ctgaccgtca taccactgga actgtgtaat tccataggca ctcaccacca
     3601 gcacacaccc gagaagaaga tacgtcagaa gatcgcgcgc ggtgcgctcc ctgaagaaat
     3661 acagcaccgc atagaaaatg accacctgct gtaggagttt gccaaactcc ttcatgctat
     3721 aggcaggatg tgccgaaaag ggaatcgtga aagccaccca agcaacaaac gcgatcaatg
     3781 gtaaatcgac ggggctagac acaaaaaagt ggcggcgacg aagtaggcac cacagcggca
     3841 ggagcaccag gagtactaag aagatgttgc gttcaaccac taagagtggc aggaacggca
     3901 gggagaaaat atacacataa agcaaggccc ggacaatcag atccagagtt ggtccgtgat
     3961 ttgcttccat ggaggcccat acagcgggaa tccgactaat cttcatacat tcgcccctgc
     4021 tgcccagcac gctgctccat cagaccgact cccacagcca acccagcaaa acccagtatc
     4081 cagacgacca aaatcgcaag ccgccccgaa tcggtggctc cgtgataaag cactgcggcg
     4141 gccacgcaca gtgccatcag cgcatattct gacagaagcg tttttcgatg tccccacccg
     4201 tggagaacca accgctggta atagtgggtt cgatgggctt cccaaatttt ctctccgcgc
     4261 acggcccgcc ccaacatggt ggtggttgcg tccagcacaa acggcgaaaa caccaagagt
     4321 ggcgtccaga tatcaaacag gccatcctgc acgcccaaga cactcaatgt gccgcttaaa
     4381 aagccgagcg aaatactgcc gacatccccc atgaaaatgc gcgctggagg aagattatag
     4441 agaagaaacc cgacggctcc catcgaaata atgagggaaa gaatgagaat gaatggatgt
     4501 tgtgcctgcc aggcaaagta ggctatcgca ccaaatccaa acacggtcat cccgcccgca
     4561 aatccgtcca ttccatccat aaaattgtaa agattcgtca tccaaaggac aaacaagaga
     4621 gtcaggggga ccgcaacgcc ctcgaaggag aggctaccga cagcaggcag ggacagagac
     4681 gacaatgaaa cgccagctcc gatgacgact gtcgcggcgg caatcagttg aacggctaac
     4741 cgaaggagca caggcaatcc gtcacggtcg tcccagaacg acaccactgc cagcagaact
     4801 gtcatggcca aaatccacgc ccctgccgtt ggggaagtct gcgaggcgct taaccaccat
     4861 gcctgggtag gaaattccca gagggttgct actatcaacc cgatggccgc gctaacaagg
     4921 agcgcgaggc cgcctgtgcg tggcgtggga atctcatgca gtgatcgcgc attggggtag
     4981 tccaacagat aaagacgaga accgggcatg cacaaccaac cgattcccca gtacgtcaag
     5041 acaagcacga ggccaccgac tcctaacagc gccacttctc gattccttcc agagtcagcc
     5101 agcatcacta ttgatgctga agataatatt ccgccgctcg gcgcaattcc tcgtcgacac
     5161 gtatcggggg gcaccacccc agcaaggtac gcgcttttgt aatattcact cgtagattcc
     5221 cgcagagacg agcagccatc tctcgctgac cgagcagagt taatcccgat ctcaatgctg
     5281 gcatgggaac cggaatgagt cgtgccggac gattcatggc aagggccagc cgatcaatca
     5341 attctgtcgt ggagagatcc tcaccatcgc tcacgaagaa tatctcattg gcagcgcgtg
     5401 ggtgctcgat gcaagtgacg atgaaatcaa cgagattccc caaccccact agactgcggc
     5461 ggttacggat cgctcctagc ggcaagggaa taccacgggc cagcgcttgc atcaacaact
     5521 gaaaattggc ccttactcct ggcccgtaga cgagaggcgg ccgcacgacc accaccttga
     5581 gggcggtatg cgcggctatc tcccgcaaac cgacctccgc ctcgtacttg ctcaccccgt
     5641 aggcgtcctg gggtgccggc acatcggttt cgcggtacgc gtcctgtccg ccctctccat
     5701 tgaccttcac agagctcacg tagattagcc gctctacccc cagttccgcc gcctgacgcg
     5761 cgagagcgac cgtaccgcag acattggccg tgcgaaatgc tggcaacgga tctgcgtcgg
     5821 tttcttgcat gacatgcacg cgcgccgcgc agtgcacgat cacatgcact ccagtcagag
     5881 cctcgtgcca gtcggtatcg gggcccagat cacgaacctg gcaataggcc agaccacgca
     5941 ctggctgggc cgccgggcgc cgtaccgcgg cagtgacagt cacgccctgc gcgaccagtt
     6001 gagcgcagac agcgtgtccc acaaagccgg atgcaccggt caccaagacg ttcacggcgt
     6061 gagttccaga taaacagcca gggtctcctt gatgatccgc tcttgagaaa actctgccaa
     6121 cacaatctcc cgggcacgtc ggcccattgt ggcacggagc gctgcgtctt caatgagtct
     6181 ggccagggcc agagcgagag cttgcgcgtt acggatggga accagaaagc cattgtcacc
     6241 gtcgtgcacc acatcccgac atccaggagc atcggtggtg actagcggaa gtccgcaggc
     6301 cgccgcttcc aacagcacct tcggcagccc ttcccggtac gaaggcaggc aggcgatgtg
     6361 cccgagttgc aagaccgcag gcatatcttc gcgccgcccc caccactcaa tgccgtgctg
     6421 accatgccag gcgcgtaggg aagtatccgg cactgaggcc ggattgccgg gatccggatc
     6481 acctaccagc acaaatcggg ccttcacccc tgcatccgtc aacacatgcg cggcctcaac
     6541 gaattcacgc acccccttat ccaacaacat tcgcgccgcg aatacgaccg tgacgggctc
     6601 agacggcaat tgggaagggg gagcaaacac ctccgtatcc acccctgcac cgcggatcag
     6661 gcgcagacga tcttccggca ctcccttttg ggtcaacaaa ccaaaatcgt ccgggttctg
     6721 tacgatcgtg cagctggacg acgccccaag caaccatacc agcagcctgc aaaaggcggg
     6781 gcgcaaagcc ttgacggcaa gtgtatttga cgtgaataac cagcccagtc ccgcaacggc
     6841 attgaccaca tgaggcacgg cagcgatctt agctgcaagc gccccataga gcaccggctt
     6901 cagtgcgacg tgatgcacga tgtccggccg ctcccgccga tacaggcgcg cgagcatggc
     6961 aatttcttgc agcggattgc cgcggcgacg ggagaggtga cagggaataa ggcgaatccc
     7021 ctcagcccga atgcgttctc cgtgctcccc ttcgcgggta gccaccacca cgtcgaagcc
     7081 cgccttctga gctgccaggg cgagcggcag ccggtgggag acgaaatacc agtcctcggt
     7141 aacgacaaag agtaatttgg gacacctact catgcatgcc tacggtgggc atacggccgc
     7201 gtcagcacgg ctcctaacac gagccaaagg atgcgctgat aaaatatttc gtgagcgagg
     7261 ctaaatacgg cgaaggccac cagcagtagc aacaaaatgc aatttgcggg ctgggtagtt
     7321 cccgaccgga tcgcgtgatg ggccagcagg aaaaatgtcc agccgaacac agcgcctccc
     7381 agcaggccga actcggccag aatccacaac ggtgtgctat ggatgacctg tgggtgtccc
     7441 gcccacatcg gactcttctc gttaaacact cctaatccga cacccagaac tggcgattgc
     7501 tgccacattt ccaagccgtg ggtcaatgtt tcccaacgct ccagattact gctctcgtaa
     7561 gatagtttac tctggacaga catgtttcca tatttttgtg gcaccaatag gactatggac
     7621 cagagcagtg ccgcgaatat catccccagc cgagcatttt gtggtcggcc atgccgccga
     7681 gccaagcaag aaccagtgcc atcgctccca ccagcatagc cgttcgcgaa ccggtccaga
     7741 ccagaccagc caataagatg ccaagcagca aagaaaatat ccatggtcgt actgagcgtg
     7801 gcacacgaat gttgtgtctt gcatacaaat gagaatagcc caaaaagagc gccatgcccg
     7861 ccagcacctg gagcgcaaaa gcattgcggt tccccgcata cccctcgaag ttttgtgtaa
     7921 gttgcgcggc ggcatttacc ccccagtggg acaaaagacg cagggcgact tgcaacatca
     7981 cgaccacgac agcagtagca attagagttt cggcgaagcg ccgcagtccg tgagtcccga
     8041 cgttcgccac gatcacatac cccgacgaaa gatagccaag cagcaccaac caccccagga
     8101 gccgccctcc cagcgcccac tgcgtgatgc ctatttccaa ccaaccgtgt acgaacccca
     8161 ccagcaacag taaactgatc acgcacaacg cgaagttgaa ttggggaact ctccataccg
     8221 gaaactgccg ggcgaacaac gcatgcaggc tcaccgccgc cagagccaga atggcgaagg
     8281 gatctgccag gttcaggttg ataggcccac cagacaggga tacgtgaacc tgaaaaaaca
     8341 ccgcgaccgc caccaccgta cccaaaaccc aagcagtaaa ctcttcaatc tccgacaccg
     8401 aatgaatcat caccgctggt ggtttaggca aatcagacat gagagagttt ctgaaacaga
     8461 acggtgagga cgcgagaatg accagcgtag aacacaaccc caccatgacc gacatcgcca
     8521 gggcatctgc cgcggggata cccagaaggc cgaacacgta caccgccgca acctcgcgca
     8581 cgccccaacc tccgacagta atcggcatgc tggcagccaa gctaataagg gccgacgcgg
     8641 cgaacaagga tgcgatggga atatcctggc tgacggccag aatccctact acgaaacagg
     8701 ccagcatcag gagctggcct gtaagggtca gacctgcgat tgacattaca tgttcaagat
     8761 tcgtccacga cagcgtccgt ctggacaatt tcctctcaaa cctcgaacct ccaagccaga
     8821 gactcaatgc ccaacccccg attgcggcaa ccacaatctc gggaagtgcg atctggttaa
     8881 aaaaatcact aaccgcagat cttccgagca aatagaccgc gcccagagtc ccgagcactc
     8941 cgctgagaag cgccagcagg gcacgttcat aggcagccag actggcattc accacaggtt
     9001 gtacgccaaa actgttcagc actccttggc gtcccatgat ctgtccgaac agcggaatca
     9061 cgaagagccc ggcgacggta ccggcaatgc tggcgcgcaa agcgatgtcc cagggtagcg
     9121 caatgccaaa atgcgccaat acccgccaaa accggaacga taccacaaac agattcgctg
     9181 tcagcaatgc cagcatgctt gccacagccc agggaggaaa tccggccagc ctccgcaaga
     9241 cgtcctcaac gccagctgac cagagcacaa atgcgatcag aaatatcgag acaaaaaccg
     9301 cgggcaggcg acgaaaggag aaattactga gcaagaatat gagctccgtt gtgtgccgag
     9361 tcgcccatac tctccaacca tgcctggaac gagagcacat cccaaaggta ataggcccaa
     9421 ttccgccggc cagtcaggtg ttccagccat ttcgtgcgaa tcgcttgagg gtcaaaatag
     9481 ccctcgcgtt gtaggcgcga ctcatccaat aactcctcgg cccaaccttt caacggtcca
     9541 cgcagccaca catcgatggg tacaccaaac cccatcttgg ggcggtcgat cagtgccttc
     9601 ggcacatgcc gataga
//
LOCUS       DAOQEW010000145         9615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2276_length_9615_cov_7.370188, whole genome shotgun sequence.
ACCESSION   DAOQEW010000145 DAOQEW010000000
VERSION     DAOQEW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2276_length_9615_cov_7.370188"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1209..1505
                     /locus_tag="PLS35_14170"
     CDS             1209..1505
                     /locus_tag="PLS35_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNI19865.1"
                     /translation="MAKTLSLSEVKTRLPELVAGVQEREEEVIVTKNGRPAAILINVE
                     EYTRLKETLDILSDPGLMSQIAESRAFYKTKHTGLSFEDVFGEPLTPVKKRRTA"
     gene            1502..1795
                     /locus_tag="PLS35_14175"
     CDS             1502..1795
                     /locus_tag="PLS35_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNI19866.1"
                     /translation="MTPFRPSIPPHIAAVIRTLHPDLKQSIKSAIRAIAANPECGEPL
                     QRELDGLRKYRVRRFRIVYAVEQKRRVIRLMAVGHRRSVYEELTDRIRRAARD"
     gene            complement(1829..2983)
                     /locus_tag="PLS35_14180"
     CDS             complement(1829..2983)
                     /locus_tag="PLS35_14180"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HNI19867.1"
                     /translation="MMTRIVLLVLDGFGIGSLPDAESYGDGGCNTLQRLAAISKGLAL
                     PNLEQLGLGLLGQFQGIRPMAQPEGCYGMLGFTTKGKHSLAGHWELAGYVIEDGPTPL
                     EAFTSELASGLEAALGQKTLGNCRTVHLEPIAEFGAQHQKSGMPIAWIDTAGTIMLAA
                     HEQVIPPEELYRLAREARRRLKATMPIVRVVAHPFTGQPGQFAITERRRDFAVEAPGL
                     TLLDHLSQASQLVFGVGKVGDLFSGRGVTRSVPLFQAHAVMDEVIGMFSKAPRGLIYA
                     NLPIMSPDLQDTLSTLQHVDRRLRDLQECLKVGDVLIITGDHGFDCARPNPGHSREYV
                     PCLVTGPRLARGVNLGTRTTAADLAQTIGEALGAMRLPWGDSFLDALQSR"
     gene            complement(3072..3764)
                     /gene="deoC"
                     /locus_tag="PLS35_14185"
     CDS             complement(3072..3764)
                     /gene="deoC"
                     /locus_tag="PLS35_14185"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNI19868.1"
                     /translation="MGLVSWNENLPALLDHTVLRPEATKADVLRLCAEAKEQGFVVIF
                     VPPCYVDEAVAAVAGTAVQVGIPIGFPLGGHSTHAKVTEAIEAVAHGARVLDMVINIS
                     RLKSGDYDFVRQDMAAVVRATPGVNHKVILETCLLTREEKITACRLAVEAGMDYVKTS
                     TGFNQAGATVEDVRLMKEAVAGQAKVKASGGIRDWKSTRALLEAGADRIGTSASLKIL
                     SEWRAEQAVMTR"
     gene            complement(3850..4863)
                     /gene="mltG"
                     /locus_tag="PLS35_14190"
     CDS             complement(3850..4863)
                     /gene="mltG"
                     /locus_tag="PLS35_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNI19869.1"
                     /translation="MVILLALSGYLVLRWAQSPVASGLPKPPSKIVTIPDGSTFQQVA
                     GMLKSEQLIRSRWAFLLLGRSHDSDRKVRPGEYELDGGMSPQDILSKLLAGRVVLHPV
                     TIPEGYNLAQIAEVLAGQQVTDAKEFSKLVRDRTFIATLGIEADSLEGYLFPETYSFP
                     RQAKARDVIKAMVDGLHRVWSADLQEQAARMKLSLHQVLTLASVIEKETGSKEERELI
                     AAVFHNRLRKKIPLQSDPTVIYGLPAFDGNIHKRDLSVMSPYNTYRVQGLPPGPIASP
                     GAHSLRAALFPAQASYLYFVSRNDGTHQFSSTLAEHNLAVEKYQKQPFRKRARGSLVT
                     HGA"
     gene            complement(4787..5398)
                     /gene="ruvX"
                     /locus_tag="PLS35_14195"
     CDS             complement(4787..5398)
                     /gene="ruvX"
                     /locus_tag="PLS35_14195"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNI19870.1"
                     /translation="MKGQRILAIDHGSKRIGFALSDELGWTAQPLETFHRRNPEADIR
                     HIQELVREHEVGRVLVGLPLRLDGESGPAAKVVEEFIQLLEPALSVPVVTWDERMTTC
                     AAEDLLIAADVGRRKRKGIVDRIAAAILLQSYLASLEKPSTSQEQAISNSHEDDPWSF
                     DEPRVDDAEAIDCGTGSSDGDTLGPLGLSGPALGSKSRRQRAS"
     gene            complement(5403..8033)
                     /gene="alaS"
                     /locus_tag="PLS35_14200"
     CDS             complement(5403..8033)
                     /gene="alaS"
                     /locus_tag="PLS35_14200"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNI19871.1"
                     /translation="MSQSVNDLRRAFIRYFEQQGHRAVPSAPLIPQADPTLLFTNAGM
                     NQFKRVFLGEETRAYQRAVTVQKCLRAGGKHNDLENVGYTRRHHTFFEMLGNFSFGDY
                     FKEDAIRFGWEFLISVVGLSKDRMWITIFREDDEADRLWRKIGVSPSRIVRCGEKDNF
                     WQMADTGPCGPCSELHFDQGPSVPGDDTPNGEGDRVIEIWNLVFMQFNRDSAGTLNPL
                     PKPSIDTGMGLERLTAVAQGRLSNYDSDLFAPLLAAIGRRAGAEYGAVEQSDRSMRVI
                     ADHLRAITFLMADGVLPSNEGRGYVLRRILRRAARHGRLLGITEPFLHELTATVVDQM
                     GEAYHELRPAAGTVAEATRGEEERFIATLDQGLPILNDMLSKVKTSGQHVLQGTEIFK
                     LYDTYGFPMDLIAEACREQGITLDETGFEAAIEEQRTRARKTGGFESETARPALSDVA
                     ARVGTTAFVGYDRLDSEGVVQALLQSDRLIKEAREGDEIEIVLDVTPFYAEGGGQAGD
                     QGVLSGTDGRVEIRETTRPVPTLIVHKGVVTSGSIREGERLQLSVNPRTRKDAARNHT
                     ATHLVHAALRDLLGPHVKQYGSLVAPNRLRFDFAHFRPMSSRDIDEIESIVNEQVRLD
                     QPVQTDVMGVQDAVAGGALAFFGDKYGDRVRVVQIDTFSKELCGGTHCGRTGEIGLFR
                     IVSESGVAAGVRRIECLTGSGALDSLKRLEADVREMSELLKVAPTEVVARTRKLSEQL
                     KDKERELADVKLKMASTSSGDAQAREIKGVQVHAQRTDGLDVNGMRALADQLRDKLRS
                     GVVALGAANDGKVSLLVVVTKDLVGRLKAGELIKAMATEVGGTGGGRPEMAQAGGKNP
                     EGLGPALEKVFGLVQKALEG"
     gene            complement(8030..8476)
                     /locus_tag="PLS35_14205"
     CDS             complement(8030..8476)
                     /locus_tag="PLS35_14205"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNI19872.1"
                     /translation="MDFLTMAIRYLGRAERTASQVQHYVQEKGASRVQGHAVVRELQR
                     RGYLDDQAYATRWAEARLSRRPMGRERLKLELLRRGFEDTVAERALCSAYRSVSEQEL
                     ACQALEGRTTRMRPLQWVRFLRQRGFDDDTIQQVTQVDLETGLDEL"
     gene            complement(8527..>9615)
                     /gene="recA"
                     /locus_tag="PLS35_14210"
     CDS             complement(8527..>9615)
                     /gene="recA"
                     /locus_tag="PLS35_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNI19873.1"
                     /translation="RALDLALAQIEKQYGKGAVMKLGTDDRPADVPAISSGSLGLDIA
                     LGVGGFPRGRVIEIFGPESSGKTTLTLHAIAEAQKAGGVAAFIDAEHALDLTYAKKLG
                     VHTDDLLVSQPDTGEQALEIAETLVRSGAIDVIVVDSVAALVPRAEIEGEMGDAHMGL
                     QARLMSQALRKLTAAIAKSQTTLIFINQIRMKIGVMFGNPETTTGGNALKFYSSVRLD
                     IRRIESIKDGQDVTGSRVRVKVVKNKMAPPFRQAEFDIMFAEGISKSGEIVDMGVEKR
                     VVEKAGAWYSYKGERLGQGREAVRDFLKTNPAIAKEIEGKVRELAGLPLRGAEKKAEA
                     KEAKEEKPERKVEIRQDEKRGHSARVTS"
BASE COUNT         1957 a         3094 c         2552 g         2012 t
ORIGIN      
        1 ccatcaagct gaacgataaa gtggagcaac tcaacaccac cgtcgccaag cagcagctgc
       61 acattgaaga cctcaacgcc cgagtaattc gcctcgaagc cgcactggaa ttcgcgctcc
      121 acgcgcaacg ttcccggcgt ttgcccccat cgccccgtta gcctgcacag agaggcccac
      181 tggagatctt ttcaaccgag acaatgtcac agcgagacgt gacgtcggtg tccgtttttc
      241 tggtgagatc ccgtcgtcgt attggtacgc gcagttagga ttcgccatca gctgattgcc
      301 ggcagtcacc acgttcccgc tggtgactcg attccccaac gcatcatagg caaacgtctg
      361 cggatcccgc agcaaacgat gacttgcgct ggtgagacga tccaaggtgc agacagcatg
      421 agagcgagat tatggggcgg gaactaggcc cctgtgcaca attcagcctt ctgtgcatat
      481 gccatccttt taagtctata catccagcct agctgaaccg attgtagctt gtcctcttta
      541 aaggcaagcc acacttcagt ggcgtcccct tcctcattta tcacatgggt acctcggtga
      601 gtcggaacgt ctagcctgat tatctttcgc ccgctcagtc tctcaacctc ctgagggctc
      661 atcccacacc gcgactttgt ttcgagcagt tcagcccatc ggtcttggcc gcaagcacat
      721 cctgcaagga aaccgagaat tccaatgcca aaaaagacaa ctttcctctt ggtcatcatt
      781 attgcaccca aggtttgttg gaggcttata tggtcccatc attccactac cgactccctc
      841 agaccatccg gggtggaagc agcaccgcct ggagcacgtt gctgccgtca tactccagca
      901 gaatgtttct cccaacgttg ataagacggt agccgctgat ccgtcttaat tgctcctgca
      961 tcgatacgca caataacagc cgttgtgtct ctatactcag gggcggctga ctgcatccct
     1021 catagatacc gtatgccctg aatcggccat acacgactta atcgcagaat ccaaaaccaa
     1081 cggcacagaa tactgccggt gatcaatcgt gaatcaaggc ctgatttcac atccgcgcat
     1141 tgaccgctcc ccgctcattg cggtagctta gtgactagaa ttttggtctt ttattcacga
     1201 ggtcgattat ggcaaaaact ttgtcgttgt cggaggtcaa aacgcgcttg cctgaattgg
     1261 tagccggcgt gcaagagcgc gaagaggaag tcatcgtcac gaaaaatggt cggccagctg
     1321 ccatcttgat taacgtcgag gaatatactc gcctcaaaga gaccttggat atcctgagcg
     1381 atcccggact catgagccag atcgccgaga gccgggcctt ttacaaaacg aaacacaccg
     1441 ggctctcgtt tgaagacgta tttggcgaac ccctcacgcc ggtcaagaag cggcgcactg
     1501 cgtgaccccc ttcaggccaa gcattccccc gcatattgca gcggtgatcc ggacgcttca
     1561 tcccgatctt aaacaatcga tcaagtcagc cattcgcgcc attgccgcca atcccgaatg
     1621 cggcgagccg ctccaacgcg aactggatgg attacgaaaa tatcgcgtgc gccgatttcg
     1681 catcgtgtat gccgttgagc aaaaaagacg cgtcatccgt ctgatggccg tcggccaccg
     1741 tcgatctgta tatgaagaac tgaccgaccg gattcgacgt gcggcccgag attagtagcc
     1801 ggacgttgga agaaaccctc acatcacact agcgggattg cagggcatcc agaaaactgt
     1861 caccccaggg cagcctcatc gctcccaatg cttccccgat cgtctgagca agatcggcgg
     1921 ccgtcgtcct ggtcccgagg ttgacgcctc tggcaaggcg tggtcccgtc accaggcaag
     1981 ggacatattc gcgtgagtgt ccgggattcg gtctggcgca gtcgaatccg tggtcgcccg
     2041 tgatgatcag gacatcgccg actttgagac actcttgcaa gtcgcgcaac cggcgatcga
     2101 cgtgttgcag cgtggacagg gtgtcctgca gatcggggct catgataggg agattcgcat
     2161 agatcaatcc gcgaggcgct ttgctgaaca tgccgatgac ttcgtccatc acggcatgcg
     2221 cctgaaacaa gggtaccgat ctggtgaccc cgcgcccgct gaaaaggtcg ccgactttac
     2281 ccaccccgaa taccagctgg ctggcttggc tcaggtgatc cagcagtgtg aggcccggtg
     2341 cttccacggc aaaatcccgg cgacgttccg taatagcaaa ttgtcccggc tggccggtaa
     2401 acggatgggc caccacgcga acgatcggca tcgtcgcctt gagccggcgg cgagcctcgc
     2461 gcgccaggcg atacaactcc tcaggcggga tgacctgttc gtgggcggcc agcatgatgg
     2521 tcccggcggt atcgatccac gcgatgggca tgcctgactt ctgatgctgc gcgccgaact
     2581 ctgcaatggg ctccaggtga acggtgcgac agttccccag cgtcttttgt cccaatgcgg
     2641 cttcgagacc ggaggccagc tcgctcgtga acgcctcgag aggggtcgga ccatcttcga
     2701 tgacataccc agccaattcc caatgtccgg ccagcgagtg tttacccttg gtcgtaaacc
     2761 ccagcatgcc gtagcagccc tccggttgag ccatggggcg gataccttga aattggccca
     2821 ggagaccgag gccgagttgt tccagattcg ggagggcaag tcctttggaa atcgcagcca
     2881 gccgctgcag cgtattgcag ccgccatcgc catacgattc cgcatcgggc aaagacccga
     2941 tgccaaatcc atctaagacc agcaggacga ttcgagtcat catcgaggca ctatatcaaa
     3001 tccgctgaaa cgagcaagcc aatctcaccg tccgagcaca gtgacggccg ttccttcgta
     3061 cccagtggga ctcagcgagt catgacggcc tgttccgcac gccactcgct caggattttc
     3121 agactggcgc tggtgccgat ccgatccgcc ccagcctcca gtaacgctcg tgtcgacttc
     3181 caatcccgaa tgccgccaga agcctttacc ttcgcctgtc cggccacggc ctctttcatg
     3241 agccgcacat cttccaccgt ggcgccggct tggttgaatc ccgtggaggt cttcacatag
     3301 tccatacccg cttcaacggc taggcggcac gccgtaattt tttcttcgcg tgtgagcaaa
     3361 caggtttcca gaatgacctt atggttgact ccaggcgtgg cgcgcacgac ggccgccata
     3421 tcctgacgta cgaaatcata gtctccggat ttcaggcggc tgatgttgat gaccatgtcc
     3481 aacactcgcg cgccatgcgc caccgcttcg atcgcctcgg tcactttcgc atgggtcgaa
     3541 tggccaccga gagggaaccc gatcggaatg cccacttgca cggcggttcc ggccacggcc
     3601 gcaaccgcct catcgacata acagggcggc acaaaaatca cgacaaatcc ctgctccttc
     3661 gcctcggcac acagccgcaa gacgtcggcc tttgtcgcct cgggacgcaa taccgtgtga
     3721 tccagcagag caggtaaatt ctcattccac gacacaagtc ccatgcgcga ttcctttcag
     3781 atgatgaatt ttgatggatg taatagaccg tcacgaggcg atgcggcgtg gagtaaggca
     3841 ccaataagat cacgcgccgt gagtcaccag gcttcccctg gcccgcttgc ggaacggctg
     3901 cttctgatac ttttccacgg cgagattatg ttcggccagg gttgaggaga actgatgggt
     3961 gccatcgttg cgcgagacga aatagagata ggacgcctgc gccggaaaca gtgctgcacg
     4021 aagcgagtga gctcctggac tggcgatcgg gccgggcgga agtccctgca cacgatacgt
     4081 attgtagggg ctcatgacgg acaggtcgcg tttatgaata ttgccgtcga acgccgggag
     4141 cccatagatg accgtcggat cgctctggag gggaatcttc tttcgcaacc ggttgtgaaa
     4201 caccgccgca atcaattccc gctcttcctt ggagccggtc tctttttcga tgactgatgc
     4261 cagcgtcaag acttgatgga gcgacaactt catgcgcgcg gcctgttcct gcaagtcggc
     4321 gctccatacg cgatggagcc cgtccaccat ggccttgatg acatcacggg ccttggcttg
     4381 gcgaggaaag gaataggtct ccggaaacaa gtagccttcc agcgagtccg cttcaatccc
     4441 tagcgtcgca atgaacgtgc gatcgcgcac cagcttcgag aattccttcg catccgtcac
     4501 ctgctgtccc gccagcacct cggcgatttg cgcaagattg tatccttcgg ggatcgtgac
     4561 cggatgcaat acgacccggc ccgccaacag cttactcaga atgtcttggg gcgacatccc
     4621 gccatcaagt tcatattcgc cgggccgaac tttacgatca ctgtcatgcg accggccaag
     4681 caggagaaac gcccagcggc tgcgaatcag ctgctcgctc ttcaacatgc ccgcaacctg
     4741 ctgaaaggta ctgccgtcag gaatcgtgac gatcttggag ggtggcttag gaagcccgct
     4801 ggcgacggga ctttgagccc agcgcaggac cagatagccc gagagggcca agagtatcac
     4861 catcgctaga accagtcccg caatcaatcg cttctgcatc atcgactcgt ggttcgtcaa
     4921 aagaccaggg atcgtcttcg tgggaattgc ttatggcctg ctcttggctc gtcgacggct
     4981 tctccaaact cgcgaggtaa ctctggagga gaatcgcggc agcgatgcga tcgacgatgc
     5041 ctttccgttt gcggcggccg acatcggcgg cgatcaataa gtcctccgcc gcacaggtcg
     5101 tcatccgttc gtcccaggtt accaccggaa ccgacaaggc cggttccagc agttggatga
     5161 actcctcgac gacttttgcg gcggggccac tttccccgtc caatcgaagc ggtaagccga
     5221 cgagcacccg gccgacctcg tgctcgcgca ccaactcctg aatatgtcga atatcggcct
     5281 cgggattgcg tcgatgaaac gtttccaacg gctgggcggt ccacccgagc tcatcgctca
     5341 gggcaaagcc gatgcgtttg gagccgtgat cgatcgcaag aatccgttgg cctttcatca
     5401 gtctaccctt ccaaggcctt ttggaccaac ccaaaaactt tttcgagcgc cggtcccagc
     5461 ccttcgggat tttttccgcc ggcttgcgcc atctcagggc gaccaccgcc tgtcccgccg
     5521 acttcggtcg ccatcgcctt gatcaattca cccgccttca aacggccgac cagatccttc
     5581 gtcaccacca ccagcaacga gaccttgccg tcgttcgccg cgcccaaagc gacaacaccg
     5641 cttcggagct tgtcccgtag ttgatcggcg agcgcccgca tgccgtttac atcgaggcca
     5701 tccgtacgtt gcgcgtgcac ctggacgccc ttgatctctc gtgcctgcgc gtcaccggac
     5761 gatgtgctcg ccattttcag cttcacgtct gcaagttcgc gttccttgtc cttcagctgt
     5821 tcgctcaact tccgggtgcg cgccacaacc tccgtcggcg cgactttcag aagttcagac
     5881 atctctcgca catccgcttc caggcgcttg agggaatcca aggcaccact ccccgtcaaa
     5941 cactcgatgc ggcgcacccc ggcggctact cccgattcag agacgatccg gaacaatccg
     6001 atttcccccg tacgcccaca atgagtgccc ccgcacaatt ccttgctgaa ggtgtcgatc
     6061 tggaccactc gcacccgatc gccatacttg tccccaaaga aggccaatgc cccgcccgcc
     6121 acggcatcct gcacgcccat cacatcggtt tgcaccggct gatcaaggcg gacctgctca
     6181 ttaacgatcg attcgatctc gtcgatgtca cgggacgaca tgggccggaa atgggcaaag
     6241 tcgaaccgca gacgattcgg tgccacgagc gagccgtact gtttgacgtg cggcccaagt
     6301 agatcgcgca acgcggcatg cactaaatgg gtagcggtat ggttacgcgc cgcatctttc
     6361 ctggtccgag gattcaccga gagctgcagc cgttcgcctt cacgaataga accggatgtc
     6421 accacgccct tatggacgat caacgtcggc actggtctgg tggtctcgcg aatctccacg
     6481 cggccgtcgg tcccgcttaa cacaccttga tcgccggcct ggccgccgcc ttctgcataa
     6541 aatggcgtga cgtcgagcac gatctcgatt tcgtcgcctt cccgggcttc cttgatcaag
     6601 cgatcactct gaaggagcgc ctgcaccaca ccttcggaat ccaagcggtc atagccgaca
     6661 aatgcggtgg ttccgacacg cgccgccacg tcgctcaggg ccggcctcgc cgtttcgctc
     6721 tcgaagcccc ccgtttttct ggcgcgcgtc cgctgctctt cgatggccgc ttcaaagccg
     6781 gtttcgtcga gggtgattcc ctgttcacgg caggcctccg cgatgaggtc catcggaaac
     6841 ccgtacgtgt cgtagagttt gaaaatttcc gtcccctgca acacatgctg ccccgacgtc
     6901 ttcaccttcg acagcatgtc gttcaggatc ggcaaccctt gatccagtgt ggcgataaac
     6961 cgctcctcct caccgcgcgt cgcttcagcc accgtcccgg ccgccggccg caattcgtga
     7021 taggcctcgc ccatttggtc cacgaccgtc gccgtcaatt cgtgcaggaa cggctcggtg
     7081 ataccgagca accgtccgtg acgggccgct cgccgaagaa ttcgccgaag cacatagccc
     7141 cgcccttcat tcgacggcag gacaccgtca gccatcagaa acgtaatggc tcgaaggtgg
     7201 tcggcaatga cccgcatgga ccgatctgac tgctccaccg ctccgtactc agcaccggct
     7261 cgccgtccaa tcgcggccag caatggcgcg aacaaatcac tgtcgtaatt gctgagccgg
     7321 ccctgcgcga ccgccgtgag tcgctccagt cccatgccgg tgtcgatgct cggctttgga
     7381 agcggattga gcgtcccggc gctatcgcga ttgaattgca tgaacaccag gttccagatc
     7441 tcaatgaccc ggtcgccttc accgttcggc gtatcatcgc ccggcaccga cggcccctga
     7501 tcgaagtgca gttccgagca aggcccgcag ggacccgtgt cggccatctg ccagaaattg
     7561 tccttctcgc cacaacgcac gatacggctc ggggacacgc cgatcttccg ccacagccga
     7621 tcggcttcgt cgtcttcgcg aaagatcgtg atccacatcc ggtctttcga cagcccgacc
     7681 accgagatca gaaattccca gccgaagcgg atggcgtctt ccttgaaata atcgccgaag
     7741 gaaaagttgc cgagcatttc gaagaacgta tgatgccgcc tggtgtagcc gacattttca
     7801 aggtcattgt gcttgccgcc ggcacgcaga cacttctgca cggtcacggc ccgctgatag
     7861 gcgcgcgttt cttcacccag gaacacccgt ttgaattgat tcatcccggc gttcgtaaac
     7921 agcaacgtgg gatccgcctg tggaatcaac ggcgcgctcg gcaccgctcg atggccctgc
     7981 tgctcaaagt accggatgaa ggctcgccgc agatcgttta cactttggct cataactcgt
     8041 ctaaccccgt ctccaagtcg acttgcgtga cttgctgaat ggtgtcgtca tcaaacccgc
     8101 gttgccgcaa aaatctcacc cactgtagtg gacgcatcct cgtcgttcgt ccttctaacg
     8161 cctggcacgc caattcctgt tcggaaaccg accggtaagc actgcacaag gcccgctccg
     8221 ccaccgtatc ttcgaagcct cgacgaagga gttccagctt cagtcgttca cgtcccatcg
     8281 ggcgtcgcga gagccgggct tccgcccacc gggtggcata ggcttgatcg tcgagatacc
     8341 cgcgccgctg taactcacgg acaaccgcgt gcccctgcac ccggctcgcc cctttttctt
     8401 gaacgtagtg ctggacctga gaggctgtcc gctcagcacg gccaaggtac cggatcgcca
     8461 ttgtcaggaa atccatcggc gactccctgc gtcgtggtac accacgtcca gccgacatcc
     8521 tgctgcctat gatgtcactc tcgcactgtg gccgcgcttc tcgtcctggc gaatctcaac
     8581 cttgcgctcg ggtttctctt cttttgcctc tttggcctcg gctttcttct cggccccgcg
     8641 caaaggcagc ccggctagtt cgcgcacctt gccttcgatc tccttggcga tggccgggtt
     8701 ggtcttgaga aagtcgcgga cggcttcacg cccctggccc aatcgctcgc ccttatagga
     8761 gtaccaggcg ccggccttct ccaccacgcg cttctcgact cccatatcga caatctcgcc
     8821 cgatttggaa attccctcgg cgaacatgat gtcgaattcc gcctgtctga acggcggggc
     8881 catcttgttc ttgaccacct tgacgcggac gcggcttccg gtgacatcct gcccatcctt
     8941 gatcgactca atccgacgaa tgtcgaggcg cacggacgaa taaaacttca aggcgttgcc
     9001 gcccgtcgtg gtttccggat tgccgaacat cacaccgatt ttcatgcgga tctggttgat
     9061 gaagatcagg gtcgtctgcg atttggcgat ggcagccgtc agtttacgca aggcctggga
     9121 catcagtcgt gcctgcaacc ccatatgggc atcacccatt tcgccttcga tctcagctcg
     9181 aggcaccagc gccgcgacgg agtccacgac aatcacgtca atggcgccgc tgcggacgag
     9241 ggtctccgca atctccagcg cctgctcgcc ggtatccggc tgcgacacga gcaagtcgtc
     9301 ggtgtgtacg cccaacttct tggcataggt caaatccagc gcatgttcgg cgtcgatgaa
     9361 ggccgctacg ccacccgcct tctgtgcctc ggcgatcgca tgcaacgtca gggtggtttt
     9421 accggacgac tccggtccaa aaatctcgat gacgcgccca cgagggaatc cccctacgcc
     9481 gagcgcaata tccaatccca aggatccgct cgagatcgcc ggtacgtcgg ccggtcgatc
     9541 atcggtcccc agcttcatca ccgcgccctt gccgtattgt ttttcaatct gagccagggc
     9601 caggtccaac gcgcg
//
LOCUS       DAOQEW010000146         9604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2283_length_9604_cov_9.153629, whole genome shotgun sequence.
ACCESSION   DAOQEW010000146 DAOQEW010000000
VERSION     DAOQEW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2283_length_9604_cov_9.153629"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLS35_14215"
     CDS             complement(<1..215)
                     /locus_tag="PLS35_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19874.1"
                     /translation="MSLNAISLDSLRSIPTGQKVALLGLLVAGILVGFYFYVVDPKTV
                     ELEAVQGQVAQLDTEIQNLTLKVKHLD"
     gene            complement(217..771)
                     /locus_tag="PLS35_14220"
     CDS             complement(217..771)
                     /locus_tag="PLS35_14220"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNI19875.1"
                     /translation="MIRINLLATGPRSRKAKPQWDVRAEALIGVGVLLITLTGCWFYA
                     SSLDEDIQAKQSEKQLKDKQVAQLKEQVKAVQDFEDKKKQLEAKNRIIDQLEKSRTGP
                     VKVLDHVSQSLNPLKVWLVRLNLKGNSVELEGRALTNDDVVEFVNNLRRTDQFGAIKL
                     MESRAGLDNKMNTYQFRLDLSMKG"
     gene            complement(768..1865)
                     /gene="pilM"
                     /locus_tag="PLS35_14225"
     CDS             complement(768..1865)
                     /gene="pilM"
                     /locus_tag="PLS35_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNI19876.1"
                     /translation="MLASLKHLTDMDFLSMFSPQKQLLGLDIGSSSIKLVQIKEHRGR
                     YTLQKFGVKELEPEVIVDGTVMDEGRVVSAIKELLAEHNVKLKQVAVSISGHAVIVKK
                     ITLPPMPDEELDAQVKLAAEQYIPFDINEVNLDFYVLPPSENPDEQTEMQIVLVAAKK
                     DKINELTELVKAAGLVPIVMDVDAFAVENMYGVTSPSTQEDTTILVNIGASVMNVNIV
                     GGGVSLFTRDIPLGGNRYSEAVQREMGVSYEEAEQLKKNDGDDDHTLAAVMESVNAEV
                     ASEIARTIDYFKTTSSESEIARVLLFGGGAKVKGLAQQLRDRMHVDVEIANPFNEIDT
                     SQCNVDPEQLAEMGSLAAVGVGLALRTVGDR"
     gene            complement(1879..2094)
                     /locus_tag="PLS35_14230"
     CDS             complement(1879..2094)
                     /locus_tag="PLS35_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNI19877.1"
                     /translation="MKKVNDGQKKKLYKTNEVCEMFDISRATLFRWEREGLITGPPRD
                     WRNWRLYTAENVEQIKHVMGGGRKDVA"
     gene            complement(2230..3096)
                     /gene="rapZ"
                     /locus_tag="PLS35_14235"
     CDS             complement(2230..3096)
                     /gene="rapZ"
                     /locus_tag="PLS35_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNI19878.1"
                     /translation="MAALNLVVISGLSGSGKSHALKAFEDAGYFCVDNLPPALLPTFV
                     ELCHQQGGEIKNVALGIDIRERVFFGDLAKVLAELKSQGHALQLVFLEAREEVLVRRF
                     SESRRPHPLLPHMPVVEGVRFERERLSELRKHADRVIDTSTLTVHELRDLLIRQFRQE
                     TTARRMTISLVTFGYKFGVPYDIDLLFDVRFIRNPFFVPELKALTGEDLRVQQYVFGD
                     ETAGQFLEHLQKLFAFLIPLYERDQRSYLSIGIGCTGGRHRSVTIALRLQAQFAALGY
                     DVSVTNRDLQKS"
     gene            complement(3083..3634)
                     /gene="raiA"
                     /locus_tag="PLS35_14240"
     CDS             complement(3083..3634)
                     /gene="raiA"
                     /locus_tag="PLS35_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNI19879.1"
                     /translation="MRLMITGRHVVVTPALREYIELRMERLDRYGLKLGTLQILLSVE
                     KFHHVAEVVGVVQGRRLQAKTTTEEMYASIDEVVDKLGAQLRKLKERKVNHKFGDQPR
                     VRAVARKTPRAKDDGMEVVRPRLEALTIEEAVETLNGEKLPLLMFVNALSGSIQVLQR
                     LPNGRLSLIDPASDRARVAHGRA"
     gene            complement(3741..5258)
                     /gene="rpoN"
                     /locus_tag="PLS35_14245"
     CDS             complement(3741..5258)
                     /gene="rpoN"
                     /locus_tag="PLS35_14245"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNI19880.1"
                     /translation="MKLRLDLRLSQKLIMTPQLQQAIKLLQLSRLELQQSLQQHLMEN
                     PLLDELVAETEESEETAATEREQPDTATTVAAETRGEGDDKPAESGENAEEFSASTWE
                     DYFDTDMRRGETEYSSSSKEEFPSYEQTVAKSTSLEDHLVWQLSLSGLSDREKAIGRL
                     IIGNLDDDGYLRMTLDELVAGSEYSVAEAESVLKDVQGFDPNGVAARDLSECLLLQLK
                     FLGRSQLGSLGSRPGVLKGSVLEAIVLHHLKDLEKKQYNRIAKALNISMDDVFEATRI
                     IEGLEPKPGRPFSNTQNYAIVPDVFVVKNEGEWEVLLNDDGLPRMRISPYYKQLMSSG
                     QSGSAETKAYLDDKLRAAQWVIRSIEQRNKTIVKVVSSIVKFQEGFFEHGIQHLKPLV
                     LKQVAEDIGMHESTISRVTANKYMYCPQGMLELKFFFNAGLQRADQPTDMLSSLTVRE
                     MIRQMVAAEDARKPLKDEEIAARLRTQQVLIARRTVAKYRAEDNIPSATQRRKFF"
     gene            complement(5277..5990)
                     /gene="lptB"
                     /locus_tag="PLS35_14250"
     CDS             complement(5277..5990)
                     /gene="lptB"
                     /locus_tag="PLS35_14250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNI19881.1"
                     /translation="MAASGLVKSFRGRKVVKGVSLDVQAGEVVGLLGPNGAGKTTIFD
                     MMVGLCQPDEGQIALSGNDITDLPMYKRARRGIGYLPQESSVFRRLSVEHNILAILEM
                     LGYSRSERMERVETLLKELDLVHIRKSKAYALSGGERRRLEITRALATSPLFMLLDEP
                     FAGIDPIAVADIQQIIIRLKQRHIGVLITDHNVRETLSITDRAYIINEGTILEAGPPG
                     VIEKSEMARAVYLGEGFRL"
     gene            complement(6062..6655)
                     /locus_tag="PLS35_14255"
     CDS             complement(6062..6655)
                     /locus_tag="PLS35_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNI19882.1"
                     /translation="MSCLLLSGEAVLSTHNAYAIETRGTKEPPASSAPTTITSRTMTV
                     NNQENTAIFDGAVVLTRGPLVVHSDHMVVSFHPSRNGADGKESGGPTKSKNLTNPTTA
                     ESKERDAAPAVSERSIRMVEATGRVNIKKEDGQATCQKAVYYEEQKKIVLTGEPVAWQ
                     KGTRVSGKRIIMFLDEDRSVVEGDSQVVIEGEGGGKR"
     gene            complement(6636..7208)
                     /gene="lptC"
                     /locus_tag="PLS35_14260"
     CDS             complement(6636..7208)
                     /gene="lptC"
                     /locus_tag="PLS35_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNI19883.1"
                     /translation="MWERWVRRGLLALSVVLAAFLGYLLITRSNPSSSSRSVAPVEAE
                     SADAHIQDFTFTQTKGDLVQWKVQAEQARLYEKESRAILSNVQITMYGAQGKELSLSG
                     DEGTLDTQTKNFQLSNRSTPIVVETQSGYTIQTNHLVWTDARHEIQTPDHVTILGHGL
                     QVTGRGLLGKMDTEEFKILDDVHVDVVPAS"
     gene            complement(7498..7845)
                     /gene="rplQ"
                     /locus_tag="PLS35_14265"
                     /pseudo
     CDS             complement(7498..7845)
                     /gene="rplQ"
                     /locus_tag="PLS35_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(7864..8865)
                     /locus_tag="PLS35_14270"
     CDS             complement(7864..8865)
                     /locus_tag="PLS35_14270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNI19884.1"
                     /translation="MIKAMKDFQIPMRVEVDKDTLSPTFGRFTTEAFERGFGTTVGNS
                     LRRVLLSSLTGAAVTTVKIEGVLHEFSTIPGVTEDVTSIILNVKSLRLALQGDKPKTI
                     RLKKKGPGEAKGSDITHDGDVTILTPDLHIATLDKDAALDMEMTIKHGRGFVPAERNK
                     EEGLPIGVIAIDSIFSPIKRVNFHVENARVGRMTDYDKLTLEIWTDGTISPRDALSNA
                     AGILREHLDIFINPEERSDAKPSAGSEDLSDEVNKNLYRSVNELELSVRAANCLKNAN
                     IKTIADLVQKTEAEMLKTKNFGKKSLNEIKEILTEMGLSLGMKVDALPSGDAGVRSE"
     gene            complement(8950..9576)
                     /gene="rpsD"
                     /locus_tag="PLS35_14275"
     CDS             complement(8950..9576)
                     /gene="rpsD"
                     /locus_tag="PLS35_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNI19885.1"
                     /translation="MAKYSGPVCRLCRREGEKLFLKGSRCMTEKCAIERRSYPPGQHG
                     QARQRTSDYSLQLREKQKLKRIYGLQECQFRGIFERAERQAGVTGDTLLRLLECRLDN
                     VVYRLGFGASRKEARQLVNHGHLMINGRKVKAPGALVKAGDSIEVRQKSRELIPIQGA
                     LAAVDGRGIPEWLELDRAAYKGTIRSLPTKEHIVLPVNEQMVVELYSR"
BASE COUNT         2016 a         2867 c         2400 g         2321 t
ORIGIN      
        1 tcatccaggt gtttgacctt gagcgtcagg ttctggatct cggtatccaa ctgagccacc
       61 tgcccttgca cggcttccag ttcgacggtc ttgggatcga cgacgtagaa ataaaacccg
      121 actaaaatgc ctgccaccag taagccaagc agcgcaacct tttggcctgt cggaatgctg
      181 cggaggctgt ctaagctgat cgcattcaga ctcatgttag cctttcatcg acaggtcaag
      241 tcggaattga taggtgttca tcttattgtc cagcccagcc cggctctcca tcaatttgat
      301 ggcgccgaat tgatcggtcc gccggaggtt attgacgaat tcaacaacgt catcgttcgt
      361 caaagcccgg ccctcaagct caacgctgtt ccctttgaga ttcagccgca ccagccagac
      421 tttcagggga ttcaggctct ggctcacatg atccagcact ttgaccgggc ccgtccggct
      481 cttctccagc tgatcgataa tgcggttctt cgcttcgagt tgctttttct tatcttcaaa
      541 gtcctggact gccttcacct gctctttcaa ctgcgccact tgcttgtcct tgagctgctt
      601 ttcgctttgc ttggcctgaa tgtcttcgtc gagcgatgag gcgtagaacc aacacccggt
      661 caacgtgatc agcagtactc cgacgccgat gagtgcctcg gcgcgaacat cccactgcgg
      721 cttggctttc ctggaccgag gtccagttgc gagcaagttg attctaatca tcggtccccc
      781 accgtccgca gtgcgaggcc aacccccact gccgcaagcg accccatctc ggccaactgt
      841 tcaggatcga cattgcactg tgaggtgtcg atctcattaa agggattcgc aatttccaca
      901 tcgacatgca tccgatcgcg aagctgctga gccaacccct tcaccttcgc tcccccgccg
      961 aacaacaaga cccgcgcaat ttcgctctct gaggacgtgg tcttgaaata atcgatggtg
     1021 cgtgcaatct ccgatgccac ttcggcattg acgctctcca tcaccgccgc aagtgtatga
     1081 tcgtcatctc cgtcgttttt cttgagctgc tccgcttcct cgtacgacac gcccatttct
     1141 ctctgtacgg cttccgagta gcgatttccg cccagaggga tatcacgcgt gaagagcgac
     1201 actccgccgc cgacaatgtt cacattcatg acgctggcgc cgatgttgac cagaatggtg
     1261 gtgtcctctt gagtagaggg cgaggtcacg ccatacatgt tttctacggc aaatgcgtcc
     1321 acatccatca cgatcggcac caagccggcc gccttgacca attccgtgag ttcgttgatc
     1381 ttgtctttct tcgcagccac cagcacgatc tgcatttcgg tctgctcgtc cgggttttcc
     1441 gacggcggca agacgtagaa atcgagattg acctcattaa tatcgaacgg aatgtactgt
     1501 tcagccgcca gcttcacttg ggcgtcgagt tcctcgtctg gcatgggcgg gagagtaatc
     1561 ttcttgacga tgaccgcatg accggagatc gagacggcta cctgcttcaa tttcacgttg
     1621 tgctccgcca gcaattcctt gatggccgag acgacccgcc cttcgtccat caccgtgccg
     1681 tccacaatca cctcgggctc cagttccttc acgccgaact tctgcaaggt gtagcggccc
     1741 cggtgctcct tgatctgcac cagcttgatg ctgctcgatc cgatgtccag ccccaaaagc
     1801 tgtttctggg gggaaaacat cgacagaaaa tccatgtccg tgaggtgttt cagtgaagcc
     1861 agcatgcctt tgccctctct acgcaacgtc tttgcgcccg ccacccatca catgcttgat
     1921 ctgttcgaca ttttcggccg tatagagacg ccaatttcgc caatctcgcg gcggcccggt
     1981 aatcaaccct tcgcgctccc aacgaaacaa cgtcgcgcgc gagatgtcga acatctcgca
     2041 gacctcgttg gtcttgtaga gtttcttttt ttgaccatcg ttcactttct tcacgcaccg
     2101 cctcaaacgc cactattcat cgcgtaattc gagctcacga aattaagtat agttgatata
     2161 gtccacctgt caagaaagtg gcctccgtga gggcagacag gagaaaaatg gggaaaagac
     2221 gctctgggac taggatttct gcaggtcgcg gttggtcacg gatacgtcgt aaccgagggc
     2281 ggcaaattgt gcctggaggc gcagcgcgat ggtcaccgac cgatggcgcc ctccagtaca
     2341 cccgatgccg atactgaggt agctccgttg atcccgttca tacagtggga tcagaaaggc
     2401 aaagagtttt tgcagatgtt cgaggaactg gccggcggtc tcgtctccga atacgtactg
     2461 ctgcactcgg agatcttctc ccgtcagggc tttcagctcg gggacaaaga aaggattgcg
     2521 gatgaagcgg acgtcaaaca gcaggtcgat atcgtagggc acaccgaatt tgtagccgaa
     2581 tgtgaccaat gaaatggtca tgcgtcgggc agtcgtttcc tgacgaaact gcctgatcag
     2641 cagatcgcgc agttcgtgca ccgtcagcgt ggaggtgtcg atcactcgat cagcatgttt
     2701 tcggagttcg ctgaggcgtt cgcgttcgaa ccgcacccct tccacaacag gcatatgcgg
     2761 caacagcgga tgcgggcggc gactttctga gaaccgccgg accagcactt cttcgcgggc
     2821 ttccagaaac actaactgca gggcgtgtcc ctgcgacttc aattcggcga gtacttttgc
     2881 caggtctccg aaaaataccc gttcgcgaat atcgatcccc agggccacat tcttaatctc
     2941 gccgccctgc tggtggcaca attcgacgaa tgtcggaagg agggccggag gaagattgtc
     3001 gacacaaaaa tagcctgcat cctcaaaggc tttcagcgcg tggctctttc cggacccgga
     3061 aagaccgctg atgacaacca gattaagcgc ggccatgtgc gacgcgagca cggtcgctcg
     3121 caggatcaat cagggacagt cttccgtttg gcagccgttg gagcacttga atagacccgg
     3181 aaagcgcgtt cacaaacatc aaaagcggca gcttctcccc attgagcgtc tcgaccgctt
     3241 cctcgatcgt cagggcttcg agcctgggac ggaccacttc catgccgtca tcttttgcgc
     3301 gcggggtctt gcgagcgacg gcacgtacgc gcggctgatc gccgaatttg tgattgacct
     3361 tgcgttcctt caacttgcgt agctgcgcgc cgagtttatc cacgacttca tcaatcgacg
     3421 cgtacatttc ttcggtcgtg gttttggctt gcagccggcg tccctgcacg acgcccacga
     3481 cctccgccac atgatgaaac ttttccacac tcaacaaaat ttggagcgtg cccagcttca
     3541 acccgtagcg gtcgagccgc tccatgcgta actcaatata ctctcgcaac gccggggtca
     3601 ccaccacatg ccgtcccgtg atcatcaaac gcatgccatc ctcgtttctc gggccttgca
     3661 cgcaaggctg cgttcaagcc cgttgcgacg attgttgtac cgccatagag ccatctccat
     3721 ggtgccagac ttcgcgcacc tcaaaaaaac ttccgccgtt gcgtggcaga gggaatgtta
     3781 tcctctgcgc gatatttggc tacggtccgg cgggcgatca atacttgttg ggtccgtaat
     3841 cgggcggcaa tttcttcatc tttgagcggc tttctcgcat cttcagccgc aaccatttgc
     3901 cggatcattt cccgcacggt gagtgaggac agcatatccg tcggttgatc ggcgcgttgc
     3961 aatccagcat tgaagaaaaa tttcagctcc agcatcccct gcggacaata catatacttg
     4021 ttggccgtca cccgactgat ggtggactca tgcatgccga tatcttcagc gacctgtttg
     4081 agaaccaatg gcttcaggtg ctgaatccca tgttcgaaaa acccttcctg aaacttcacg
     4141 atgctggaga caactttcac gatcgtctta ttgcgctgct cgatgctccg gatcacccac
     4201 tgcgcggcgc ggagcttgtc gtccagatac gctttggttt ccgccgagcc ggactggcct
     4261 gaggacatca attgcttata gtacgggctg atccgcatgc gcggcaggcc atcatcattc
     4321 agcaagacct cccactcccc ttcgttcttg acgacgaaga cgtcgggcac aattgcgtaa
     4381 ttctgggtgt tggagaaggg acgccctggc ttcggctcca agccttcgat gatgcgagtc
     4441 gcctcgaata catcgtccat ggaaatgttc aaggccttgg caatacggtt atattgcttc
     4501 ttttcgagat ccttcagatg atgcaggacg atggcttcaa gcaccgagcc cttcagcacc
     4561 ccaggccgag accccagaga gcccagttgg ctccgcccta aaaacttgag ttgaagcagc
     4621 aggcactcgg acaagtctct tgccgccacg ccgttgggat cgaacccttg cacatctttc
     4681 agcacggatt cggcttccgc tacggaatat tcggatcctg ccaccagttc gtccagcgtc
     4741 atccggagat agccgtcatc gtccaaattg ccgatgatga ggcggccgat ggccttttca
     4801 cgatcggata agccggacaa cgacagctgc cagaccaggt ggtcttcgag cgacgtcgac
     4861 ttggccaccg tctgctcata ggagggaaac tcttccttgg atgaggagct gtattccgtt
     4921 tcgccacggc gcatgtcggt atcgaaatag tcttcccagg tagaggcgga aaactcttcg
     4981 gcattttctc cgctctctgc cggcttgtca tcgccttccc cgcgcgtctc agcggccaca
     5041 gtcgtcgccg tatccggttg ttcgcgctca gtggctgcag tttcctcgct ctcctccgtc
     5101 tccgccacca gttcgtccag caaggggttt tccatgaggt gctgctgcag actttgttgg
     5161 agctccaagc gggacaattg cagcagcttg atcgcctgct gcaattgcgg cgtcataatc
     5221 agtttctgac taagccgaag atccagtcgc aacttcatcg ccccactccc tgcctgctac
     5281 aggcgaaatc cttcgcccaa atacaccgcc cgagccattt cacttttctc gatcacgcca
     5341 ggaggaccgg cttcgagaat cgtgccttca ttgatgatat aggctcggtc cgtaatcgag
     5401 agggtttccc tcacattgtg atcagtgatc aataccccga tatgccgttg cttcaatcga
     5461 atgatgattt gctgaatatc cgcgaccgca atcggatcga ttccggcaaa gggttcgtcc
     5521 aacagcatga agagcggact cgttgccagc gcgcgcgtaa tctccaatcg ccggcgttcc
     5581 cctccagata aggcgtaggc cttacttttt cgaatatgca ccagatcgag ctctttcagc
     5641 aacgtttcca cccgctccat ccgttcactt ctcgaatagc ctagcatctc gagaatcgca
     5701 aggatattgt gttccaccga cagccgccga aagacggacg attcttgagg caagtaacca
     5761 attcctcgcc gggcccgctt atacataggc aaatcggtaa tgtcgttgcc actcaacgca
     5821 atctgtccct catccggctg acacagtccc accatcatat cgaagatggt ggtcttcccg
     5881 gctccgttcg gccccaacag tccgaccact tcaccggcct gcacgtcgag ggagacgccc
     5941 ttgaccacct tgcgcccgcg gaagctcttc accaacccgc tggctgccaa gcgatccgct
     6001 ttcgccgata acgctgccac gccttcttgc ggcgtctggc tggtgattgg cttggtgtca
     6061 atcaccgctt gccaccgcct tcaccttcga taacgacctg ggagtcgccc tccaccacac
     6121 ttcgatcttc gtccagaaac ataatgatgc gctttccgga cacacgcgtt cccttctgcc
     6181 aggccacagg ttcgcccgtg agcacgattt ttttctgctc ctcataatac acggccttct
     6241 ggcacgtggc ttggccgtct tctttcttaa tgttcacacg accggtcgcc tcgaccatgc
     6301 gaatactgcg ttccgatacg gcaggcgctg catctcgctc cttcgattcg gccgtcgtcg
     6361 ggttcgtgag attcttggat tttgtcgggc ctccggattc tttaccatcg gcgccattgc
     6421 ggctcgggtg gaaagagacc accatgtgat ctgaatgcac aaccaacgga ccacgcgtca
     6481 agacaacggc tccatcaaaa atcgccgtgt tttcttgatt attgacggtc atcgtccggg
     6541 acgtaatcgt agtaggggca gaactggctg gaggctcttt ggtcccgcgc gtttctatcg
     6601 cgtacgcgtt gtgcgtgctt agcaccgcct cgccgctaag aagcaggcac gacatccaca
     6661 tgcacgtcgt caaggatctt gaattcctcc gtatccatct tgcccaacaa ccctcttccc
     6721 gtgacttgca atccatgccc cagaatcgtg acatggtctg gagtttgaat ttcatgccgt
     6781 gcgtcagtcc atacaaggtg atttgtctga atggtatagc cactttgtgt ctcaactaca
     6841 atcggcgtgg atctgttcga caactgaaag ttcttcgttt gtgtatccag cgtcccctcg
     6901 tccccggata gggagagttc ctttccctgc gcaccgtaca tggtaatctg aacattgctg
     6961 aggatagcgc ggctctcttt ttcgtagagg cgcgcttgct cggcttgcac tttccactga
     7021 acgagatcgc ctttggtctg ggtaaaggtg aagtcttgaa tatgcgcatc ggcagattcg
     7081 gcctcgaccg gcgcgaccga tcgcgacgaa gagctgggat tggagcgtgt aatgagcaaa
     7141 tacccaagga atgccgcaag aactacgctg agagctaaga gacctcgccg aacccaacgt
     7201 tcccacatgg aaaaaagccc ctattttaaa gagcttccca gatcagagtc tggtggcatg
     7261 atagtagcat tccccagaag ccaagtaaac aaaagggacg gcggatggcc tgagaaaatg
     7321 gtgtgagttg caagagaatc gaggcaggga ggaaccgaag gccacgacga cagtgacgtc
     7381 atcgtggcct tcttctcgtt agctttctgc aggcgctgaa cttttctcag tcgactcttt
     7441 cttctccgcc actgacgtgt cagcggcagg cgctgcctta ggcttttccg gtcgtgtaac
     7501 taactctatt gcgaccatct cagccgcatc tccgatccga cggcgagtgc ggatgatacg
     7561 cgtatagccc ccaggacggt tttggaaccg tgacgcaacg tcgctgaata acttcgacac
     7621 tacggccttg ctccgcaaga agctcaatgc gcgacgacgg gcaggcaggg tgccttcttt
     7681 gccaagggag atcattcgat cagtgaaacc acgaatttcc ttcgccttcg cttccgtcgt
     7741 ttcgatacgc tcctgctcca gcaatgaagt cacgagactt ctgaacagag cccatcggtg
     7801 tttggtttga cgtccgagct gtcgaccctt ctttttgtgg cgcacggtag tcctgttcct
     7861 ttcttactct gaacgcacgc ccgcgtcgcc tgacggcaag gcgtcgactt tcattccaag
     7921 gctcagtccc atttcggtca ggatttcctt gatttcgttg agcgacttct tcccgaaatt
     7981 cttcgtcttc aacatttctg cttcggtctt ttggaccaga tcggcaatgg tcttgatatt
     8041 ggcgttcttg aggcaatttg ccgcgcgaac ggacaattcc aattcattga cgcttcggta
     8101 cagattctta ttcacttcat cggacaaatc ttcgctacca gcggatggct tggcgtctga
     8161 acgctcttca ggattgatga agatatcgag atgttcccga agaatcccag ccgcgttgga
     8221 caatgcatcc cgcggagaaa tcgtgccgtc cgtccaaatt tcaagcgtta atttgtcata
     8281 gtccgtcatt cgaccgacgc gagcattttc gacgtggaaa tttacccgtt tgatcggaga
     8341 gaaaatggag tcgatggcaa tcacaccgat cggcaggcct tcttccttat tgcgctcggc
     8401 tggaacaaac ccgcgaccat gcttgatggt catttccata tcaagcgcag catccttgtc
     8461 cagcgtcgcg atgtgcaggt ctggcgtgag aatcgtcacg tcaccatcat gcgtaatatc
     8521 ggaacctttc gcttctcccg gtcccttctt cttgagacgg atggtcttcg gcttgtcgcc
     8581 ctgaagcgcc aagcggagac tctttacatt caaaataatc gaggtgacgt cttccgtcac
     8641 accaggaatc gtggaaaact catgtaacac accttcgatc ttgacggtgg tcaccgcagc
     8701 ccctgtcaag gaggacagca agacacggcg cagggagttt cctaccgtgg tgccgaatcc
     8761 tcgctcaaat gcctcggtcg taaatcttcc gaatgtcggg gaaagagtgt ccttgtcgac
     8821 ttccacccgc atggggatct gaaagtcttt catcgcttta atcatgactc ccccttcatt
     8881 aactcgtgtg tgcccatata tcgttcaacc atgatgaggt ccaatcaggg aatccccaac
     8941 catgtcggtt tagcgcgaat acagttcgac gaccatctgc tcatttacag gcaacacaat
     9001 atgttccttg gtcggcaagg atcgaatcgt ccccttgtag gcggccctat ccaactcgag
     9061 ccattcagga atgccacggc cgtccaccgc cgcaagtgca ccctgaatcg gaatcaattc
     9121 gcgactcttt tgccggacct cgatggaatc accagctttg acaagagcac cgggcgcctt
     9181 cacttttcgg ccattgatca tcaagtgacc atgattgacc aactgcctgg cctcttttcg
     9241 tgaggcgccg aagccgagcc gatacaccac attgtctaag cgacattcca ggaggcgcag
     9301 caaggtatcg cccgttacgc cggcttgacg ttccgctcgc tcgaaaatgc cgcggaattg
     9361 acattcctgg agaccgtaaa tcctcttcag tttctgcttt tcacgcaact gtaagctgta
     9421 gtccgacgtc cgttgacggg cctgaccgtg ctgtccagga ggataactcc ggcgctcgat
     9481 cgcgcacttc tcggtcatgc aacgagaccc cttcaaaaag agcttctcgc cctctctccg
     9541 gcacaagcgg cagacaggtc cactatattt tgccacgcta ctacccctcc tctacctcaa
     9601 caac
//
LOCUS       DAOQEW010000147         9534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2314_length_9534_cov_6.947674, whole genome shotgun sequence.
ACCESSION   DAOQEW010000147 DAOQEW010000000
VERSION     DAOQEW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2314_length_9534_cov_6.947674"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLS35_14280"
     CDS             <1..668
                     /locus_tag="PLS35_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19886.1"
                     /translation="SEEQYAQEFECSFEAAVIGAYYGKLMRQADDDKRIAGVPYEPAA
                     QVYTAWDLGIRDSTAIWFAQVVGREIHLIDYYEATGADLGHYVREIFGRNYIYAGHFV
                     PHDAQAKELGTGKSRLEVLESLGLKNITVAPMHRVEDGINAVRTIIPRCWFDARKCAR
                     GIDALKLYRAEFDDKLQALKPRPVHDWTSHAADAFRYLAMTLDGATQSHAKFEEPEED
                     WIV"
     gene            668..2887
                     /locus_tag="PLS35_14285"
     CDS             668..2887
                     /locus_tag="PLS35_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19887.1"
                     /translation="MAEFDEEELKALLSSEITSAVSYDSSELSEKKSRALEYYRGVMA
                     DTPAMEGRSQIVSRDVADTIGWMLPGIIRVFTASDRMAVYEAEKPGDEAFAQQATDYV
                     NFVFMRDNQGYRTLWDATHDSLLLANGIVKHWWDKKEECEYTEHSGLTEEQIAVLESD
                     QNVEVIAKKDGEPQVMLAPAPTGQLMEVPIQTFDVKVKRVLRNGRLKVKCIKKLLLNR
                     EATCIEDARFLSHPEDVTRSDLIEMGFDRELVENLPIDRFSTTRQEDISRNDNAVLAN
                     MVGHESMMLVELHECYVKMDVDGDGIAETVRAFYAGGPGTGELLDWEIWEDDVPFSDI
                     PCEPVPHQWEARSVFDDTEDLQRVKTVLTRQFLDNTYWNNNPMTAAEEGSVVNAETLR
                     SPRFGATVYYKKGSAPPTPLPVPYIGEKALLALQHFDNVREMRTGVSRSTMALDPEAL
                     QNQTATANQNQKDSAYSQIELIARNQAELGWRRVFRQILKLIVKHQDRPRTIRLRDTW
                     VEMDPRSWNANMDVTINIGLGTGSRDRDMAMLNQILNVQMAMTDRLAQGGFAQQALDM
                     IPKINLTATKLAESAGIKNPDQFYLDIKQDTLEQMKQQASQPKPDPAMAKVQADTQIK
                     QAELQQRDKEMVVNAQIAQQADERKAQIEAVQAEADMATEREKLQGQFALEQQKFEFE
                     KELKLLEMAMKREQHDQQMAQRAEQHRQQMESGVFKAVQGQQAHEQKMEAAKSAGDN"
     gene            2887..3198
                     /locus_tag="PLS35_14290"
     CDS             2887..3198
                     /locus_tag="PLS35_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19888.1"
                     /translation="MATLWIKEHARKPQYSGGPDIWAEPALVEQAVTISGTSAQSAAF
                     NAQTKFITITADSAFCYLVSTNPTAATTNFRVAADSILTLGVEQASGSATYKIAGITT
                     T"
     gene            3198..3437
                     /locus_tag="PLS35_14295"
     CDS             3198..3437
                     /locus_tag="PLS35_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19889.1"
                     /translation="MNLSHLAKEADRLKHDEIFNKALNDIRIEALDALAMADADDKTM
                     IVRLQQKVAVIDEIRTTLDRYILAAEVQEPAGSFA"
     gene            3469..4488
                     /locus_tag="PLS35_14300"
     CDS             3469..4488
                     /locus_tag="PLS35_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19890.1"
                     /translation="MSETNPAPAAGNDAPLSFDDGVDALTDVLKDPETDLSEEDQDQE
                     GEQNEADADGDPDAEVETEASEEETAEEETDGPGYESGKFAADTANVRLKDGTVISVQ
                     DLKRGYLSQASFTRGTQENAKEREALASQKAETEQYARTLQEHRDFLLQASKQFIPQA
                     PDRSLLDQNSASFDPVRYWSMKADYDEKISALSQLQQMAQSEQERVAKEQERQLRELR
                     AAESKKLLEDMPELAKPEVYNKFWAEAVETMADYGFSAEDLNGLSDHRFYKAMRDLAA
                     YRRARSRLPAVKQAIQSKPVLSGKRRMDTKDKSSREAQARSAQLRRTGSFDDGVRSLM
                     DIPDL"
     gene            4500..5510
                     /locus_tag="PLS35_14305"
     CDS             4500..5510
                     /locus_tag="PLS35_14305"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HNI19891.1"
                     /translation="MAQVANTFETYDAKGNREELADKIYQITPEETPYLSLIGRKPVV
                     STHPEWQTDTLASPDTSNNQPEGNDWTYDAVAATTRVGTYTQISDKKIVISRTQDKTS
                     KAGRKSELAREIAKKGVELRIDMEAICLSNQASTAGTGNGATNRKLGGFRAWLASNDS
                     MGSGGSSGGYNTSTNVVDAATNGTQRAFTKAILDAVILSTFNAGGSPKTLMLAPYAKT
                     VFSTFMSDSNVAPQRYETPSKGQTTIVAAADMYLSDFGPVSVVPNRQMARAGATIARN
                     AFLIDPRMVSLGVFDDIDLEKPAKTGDAEKRVLVTEYTLLVNNEAAHGVAADIYGLTS
                     SS"
     gene            5526..5954
                     /locus_tag="PLS35_14310"
     CDS             5526..5954
                     /locus_tag="PLS35_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19892.1"
                     /translation="MAYPLQPIAYTAAVTLDRDIHANGAIVVMNSTTGFTVTLPASAG
                     TGDVYEIFVNATAGSGNHVVQVANSTDIMQGVIHLTTDIAGTSLPTASTTDTITMNGT
                     TTGGVKGSWIRLKDVVSGVWMLDGGLVCTGTEATPFSAAV"
     gene            6010..6375
                     /locus_tag="PLS35_14315"
     CDS             6010..6375
                     /locus_tag="PLS35_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19893.1"
                     /translation="MPKGVYERKRTDQMAETPQTIAASPDKPKLFPVVLSRNYVPLGE
                     YEIVGYLKEAVRQKDAAGTWRIIEKEEFVEGEMKPAHTPGVGYPNKIWAGTHIRLPLD
                     EAKSIVAKKIAERADAIAA"
     gene            6359..6712
                     /locus_tag="PLS35_14320"
     CDS             6359..6712
                     /locus_tag="PLS35_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19894.1"
                     /translation="MPSPPDPSRIPDHVWEFEGISSDGLRRTYTCTIDGYTFRKTENL
                     VEAELLEQNRVEFNDSMTKLFRDDAIGTKVASIPLNVFYRDFAARLKEGDTDFVKHWL
                     NSDHNRPYRTFRGRI"
     gene            6713..7366
                     /locus_tag="PLS35_14325"
     CDS             6713..7366
                     /locus_tag="PLS35_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19895.1"
                     /translation="MAINTYATLVAAATEYLAREEDATLVARIPDFITLAEAKFNRVL
                     LHPRMETIDTLTVNTGGADPEYLDLPTDFQTMRRARLSGVEGKPRLQFMTRTQLDDYR
                     YSNDNTTGQPVYFTIIGDQMELVPTPNDDYDVEIVYRANVPAINSGNSSNWLLTMAPD
                     LYLYGVLLEAAPYMKADERIAVWVAGVESAIDQLNKHGDRQSFDSGPTTIRLPGVVP"
     gene            7869..9488
                     /locus_tag="PLS35_14330"
     CDS             7869..9488
                     /locus_tag="PLS35_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19896.1"
                     /translation="MLPRGDGYGPFKSFAAATQALPAACRGGFYALKSDGSVQVFAGT
                     SKGLYRLNNTDLSWVPVGKLANCTISNASPGVVTYTNTFAAGDPVVFAAGSGTLPAEI
                     VAGTVYYVHATGLSGSSFSVSATSGGTRINTSGGSGTVKITAYYSALSSNAQWQFAQF
                     GNLVFATQANEVLQVYNLSSSSQFADNSGSPPQAAYISVVGRFLVLSGLLSTPYRVHW
                     SGLNDTTNWTSGVGSSDYQDFPDGGIVRGVAGGEFGLVFQDQSIRRMSYIPGSAIIFQ
                     IERITQDLGLFAPYSIVRAGAMVYFRAGQGFYKIAPGGYPEQIGRERVDRTFLEDLDT
                     SNLQLFIGAADPRSSRVFWAYKSGAGATGLYDKLLGYDAALDRFFPLTASGEYLTGIS
                     QAGITLENLDTISSSIDALTLTLDSYASSVQPQLAQWDSNHKQGFFTGSNLEATLETA
                     EQGTDSKRIFVGGFRPVTDAPTFYGSASFRENVQDAVTSTPEVARNSRTGRCDLRKST
                     RYSRMKVRIPASTVWTYSAGVEPDFRPDGET"
BASE COUNT         2172 a         2801 c         2794 g         1767 t
ORIGIN      
        1 tgagcgaaga acaatacgcc caggaattcg agtgcagctt tgaggccgct gttatcggcg
       61 cctattacgg caagctgatg cggcaggctg atgacgacaa gcgcattgca ggggtgccat
      121 atgagccagc agctcaagtt tacactgcat gggacttggg aatcagagac agcacggcaa
      181 tttggtttgc ccaagtggtt ggacgcgaaa tccatctcat tgattactac gaagctaccg
      241 gggctgatct tggacattac gttcgggaaa ttttcggacg gaactatata tatgcaggac
      301 atttcgtccc gcacgacgcg caagctaaag agctgggaac gggcaaaagc cgcctggagg
      361 ttctggaaag ccttgggctg aaaaacatca cggtagcccc catgcaccgg gttgaggacg
      421 gtatcaacgc cgtccgaacg atcatcccga ggtgctggtt cgacgccaga aaatgcgctc
      481 gcggcatcga tgcgctgaag ctctaccgcg ctgaattcga cgacaaatta caggcattga
      541 agccgaggcc cgttcatgac tggacgagcc acgcggccga tgccttccgc tatctcgcca
      601 tgacattgga cggcgcgacg caaagccatg ccaagtttga agagcctgag gaagactgga
      661 tcgtttgatg gcggaatttg acgaggaaga gctaaaggcg ctcctctcca gtgaaatcac
      721 gtctgccgtc agctacgaca gttccgaact gtccgagaag aagtcgcgcg ccctcgaata
      781 ttaccgtggc gtcatggccg acactcctgc catggaaggg cgctcccaga tcgtttcccg
      841 cgacgttgcc gacaccatcg gctggatgct gcccggcatc atcagggtat tcacggcctc
      901 cgatagaatg gccgtatacg aggcagagaa gcccggcgac gaggctttcg cgcagcaggc
      961 cacggactac gtgaacttcg ttttcatgcg tgacaaccag ggatatcgca ccctgtggga
     1021 cgccacgcac gactcgctcc ttctcgccaa tggcatcgtc aagcattggt gggacaagaa
     1081 agaggagtgt gaatacaccg agcattccgg cctgaccgag gagcagattg cggtccttga
     1141 gtcggatcag aatgtcgagg tcatcgccaa gaaggacggc gagccccagg tgatgcttgc
     1201 gccggccccg accggccagc tcatggaagt gccgatccag acctttgacg tcaaggtcaa
     1261 gcgcgtcctt cgcaatggcc ggctgaaggt caagtgcatc aagaaattgc tgttgaaccg
     1321 ggaggccacc tgcatcgagg atgcaaggtt cctgtcgcat cctgaggacg tgacccgttc
     1381 cgacctgatc gagatgggct ttgaccggga gctggttgag aaccttccga tcgatcgctt
     1441 ctcgaccacg cggcaagagg acatatccag aaacgacaat gctgtgctgg ccaacatggt
     1501 cggccacgag tcgatgatgc tggttgagct gcacgagtgc tacgtcaaga tggacgtgga
     1561 cggtgacggc attgccgaga cggtcagggc cttttatgcc ggcggccctg gtacgggtga
     1621 gcttttggat tgggaaatct gggaggacga cgttccgttc tctgacatcc cctgcgagcc
     1681 cgtcccgcat cagtgggaag cccgctccgt ctttgacgac accgaggact tgcagcgggt
     1741 caagacggtt ctgacccgcc agttcctcga caatacctat tggaacaata atccgatgac
     1801 ggcggccgaa gagggctccg ttgtcaatgc ggaaactctg cgctctcctc gattcggcgc
     1861 gacggtctat tacaagaagg gatcggctcc cccgaccccg ctgcccgttc cctacatcgg
     1921 cgaaaaggcg ctgttggcgc ttcaacactt cgataacgtc cgcgagatgc gtaccggcgt
     1981 gtcccgctcc accatggcgc tggatcccga ggcgctgcag aaccagaccg caacggccaa
     2041 ccagaaccag aaagactcgg cttactctca aatcgagctg atcgcgcgca atcaggccga
     2101 attgggctgg agacgggtat tccgtcaaat cctgaagctg atcgtcaagc accaggacag
     2161 gccgcgaact attcgcctcc gggatacctg ggtggagatg gacccgcgtt cctggaacgc
     2221 caatatggac gttacgatca acatcgggct cgggaccggc tcgcgtgatc gtgacatggc
     2281 gatgctgaac cagattctaa acgtccagat ggcgatgacg gacaggcttg ctcaaggcgg
     2341 ctttgcccag caggcgctcg acatgattcc gaagatcaac ctgacagcaa cgaaactggc
     2401 ggaaagcgcc gggatcaaga acccggatca gttctatctc gacattaagc aggacacgct
     2461 tgagcagatg aagcagcagg cgtcgcagcc taagccggac ccggccatgg ccaaggtgca
     2521 ggccgacacg cagatcaagc aggctgagtt gcagcagcgc gacaaggaga tggtcgtcaa
     2581 cgcccagatc gcgcagcagg ccgacgaacg caaggcccag atcgaggcgg tgcaggccga
     2641 agccgacatg gcgaccgagc gcgagaagct gcaaggccag ttcgcgcttg aacagcagaa
     2701 gttcgaattc gagaaggaat tgaagctgct tgaaatggcg atgaagcgcg agcagcacga
     2761 tcagcagatg gcccagcgcg cagagcagca ccggcaacag atggaatcgg gcgtgttcaa
     2821 ggcggtgcag ggccagcagg cgcacgaaca gaagatggaa gcggcgaagt ccgcaggaga
     2881 taactgatgg cgaccctttg gatcaaggaa cacgcaagaa agccgcaata ttccggcggc
     2941 cctgacatct gggccgaacc cgcgcttgtg gaacaggcgg ttacgatcag cggcacatcg
     3001 gcacagtcgg cggcgttcaa cgcacagacc aagttcatca ccatcacggc cgacagcgca
     3061 ttttgctatc tggtctcgac caatcccacg gcggcgacga ccaacttccg cgttgcggcc
     3121 gactctatcc tgaccctcgg agttgagcag gcatcgggct ccgcgacgta caagattgcc
     3181 ggcattacca cgacctgatg aacctctccc atctcgccaa ggaagccgac aggctcaagc
     3241 acgacgagat attcaacaag gcattgaacg acatccgtat tgaggcgctt gacgcgttag
     3301 cgatggccga tgccgacgac aagacaatga tcgtgcgcct tcagcagaag gtcgccgtga
     3361 tcgacgaaat ccgcaccact ctggaccgct acatcctggc ggcagaagtg caggaaccag
     3421 caggctcctt cgcgtaagga ctccccgcta aactcaaagg aaaactgaat gtctgaaacc
     3481 aaccccgctc cggcggctgg taacgacgcc ccgttgtctt tcgatgacgg agttgacgca
     3541 cttactgatg ttctgaaaga cccggaaacg gacctctcgg aagaagatca ggatcaagag
     3601 ggcgaacaga acgaagcaga cgcggatggt gaccccgacg cggaagtaga gactgaggcg
     3661 tccgaagagg aaaccgcaga agaggaaact gacggacccg gttacgagtc aggcaagttt
     3721 gcggccgata ccgcaaatgt ccgcctgaaa gacggcaccg tgatctccgt gcaggacttg
     3781 aagcgcggct atctctcaca agcatcgttc acgcgaggca cacaggaaaa cgccaaggaa
     3841 agagaagcct tggcctccca gaaggccgaa acggaacaat acgctcgcac tttgcaggag
     3901 catcgggact ttctccttca agcgtcgaag cagttcattc cccaggcgcc ggaccgttcc
     3961 ttgctggatc agaattccgc aagtttcgac ccggttcgat actggtcgat gaaggctgac
     4021 tacgacgaaa agatcagcgc gctatcgcaa ctgcaacaga tggcccaatc cgaacaggaa
     4081 cgggtcgcca aagagcagga gcggcagctt agggaattgc gcgcggccga aagcaagaag
     4141 ttgctggaag acatgccgga actggcaaaa ccggaagtct acaacaagtt ctgggctgaa
     4201 gccgttgaga cgatggccga ttacgggttc tctgcggagg atctgaacgg cctttcggac
     4261 catcgctttt acaaggcgat gcgagacctt gcggcatatc gcagggcacg cagcaggctc
     4321 cccgctgtca agcaggccat ccagtccaag cccgttctgt cgggcaagag gcggatggat
     4381 acgaaggaca aatcctcccg agaggcacag gcaaggtcag cacaactgcg caggaccgga
     4441 tcattcgatg acggcgtgcg ctcgctcatg gacatccctg atctctaagg agaaattcaa
     4501 tggcacaggt tgccaatacg tttgagacct acgacgccaa aggcaatcgg gaagaactgg
     4561 ccgataaaat ctaccagatc acccccgagg aaacccccta cctctcgctg atcggccgca
     4621 agccggttgt gtccactcat cccgagtggc agaccgacac gctcgcctct ccggacactt
     4681 ccaacaacca gccggaaggc aacgattgga cgtatgacgc tgtagctgcc acgacccgcg
     4741 tcggcacata cacgcagatt tcggacaaga agatcgtcat ctcgcgcacc caggataaga
     4801 cctcgaaggc cggccgcaag tcggaactgg cccgcgagat cgccaagaag ggcgtcgagc
     4861 tgcgtatcga catggaggcg atctgcctga gtaatcaggc gtccaccgca ggcaccggta
     4921 acggcgccac caaccgcaag cttggcggct tccgtgcgtg gctggcctcg aacgactcga
     4981 tgggctctgg cggctcgtcc ggcggctaca acacctcgac caacgtcgtg gatgcggcca
     5041 ccaacggcac ccagcgcgcc ttcaccaagg cgatcctcga tgcggtcatc ctgtccacct
     5101 tcaacgcggg tggctcgccc aagacgctga tgctggcgcc gtatgccaag acggtgttct
     5161 cgaccttcat gtccgactcc aacgtcgctc cgcagcgtta cgagacgcct tcaaagggac
     5221 agaccaccat cgttgctgcg gccgacatgt atctgtcgga cttcggcccg gtctcggttg
     5281 ttccgaaccg tcagatggcc cgcgccggtg caacgattgc gcgcaatgcg ttcctgatcg
     5341 atccgcgcat ggtctctctg ggtgtcttcg acgacatcga cctggagaag cccgccaaga
     5401 ccggcgacgc ggaaaagcgc gttctcgtca ccgaatacac gctgttggtc aacaacgaag
     5461 ccgctcacgg cgtcgctgcg gacatctacg gcctgaccag ctcgtcctaa taggagaaac
     5521 gaacaatggc ttatcctctt cagcccatcg cctatacggc ggcggtcacg ctggaccgcg
     5581 acattcacgc taatggcgcg attgtcgtca tgaactcgac gaccggcttc acggtgacgc
     5641 tgccggcgtc tgctggaacg ggcgacgttt acgagatttt cgtgaacgcc accgccggct
     5701 ccggcaacca tgtggttcag gttgcgaact ccaccgacat catgcaaggc gtcattcacc
     5761 tgacgaccga cattgccggc acttcgctcc cgaccgcatc gaccacggac accatcacga
     5821 tgaacggcac cacgaccgga ggcgtcaagg gctcctggat caggctcaag gatgttgttt
     5881 cgggcgtgtg gatgctcgac ggcggtctgg tctgtaccgg caccgaagcc accccgttct
     5941 cggcggcggt ctaatactca aacctcaagg gcggccttcg ggccgctctt ttcttttcag
     6001 gagccagaaa tgccgaaggg cgtttacgag cgaaaaagga cagatcaaat ggcagaaacc
     6061 ccgcagacca tcgcggcttc acccgacaaa ccgaagctct ttcccgtcgt gctctcccgc
     6121 aactacgtcc ccttgggcga gtacgaaatc gtcggctacc tgaaagaggc cgtccggcaa
     6181 aaggacgccg ccggaacttg gcgcatcatc gaaaaggaag agttcgtgga gggcgagatg
     6241 aagcccgccc acactcccgg cgtcggctat cccaacaaga tttgggccgg cacgcacatc
     6301 cggcttccgc tcgatgaagc caagagcatc gttgccaaga agatcgcaga gcgggccgat
     6361 gccatcgccg cctgatccct ctcgcatccc ggatcatgtc tgggagttcg agggcatctc
     6421 cagcgacgga ctgcggcgca cctacacctg cacgattgac ggctacacgt tccgcaagac
     6481 cgagaacctc gttgaggcgg aattgctgga gcagaaccgc gtggagttca acgactccat
     6541 gaccaagctg ttcagggatg acgctatcgg aaccaaggta gcgagcattc cgctgaatgt
     6601 cttctatcgg gacttcgcgg cgcggctgaa ggaaggcgat accgatttcg tcaagcattg
     6661 gctgaacagc gaccataaca ggccctatcg aaccttccgg ggacgcatct aggtggcaat
     6721 caacacttac gcgacgctgg ttgcggcagc cacggaatat ctggcgaggg aggaggacgc
     6781 gacgctggtt gcgcgcattc ccgacttcat cacgctagca gaggccaagt tcaatcgcgt
     6841 cctgctccat ccacgaatgg agaccatcga cacgctcacg gtcaataccg gaggggccga
     6901 tcccgaatat ctggatttgc ctaccgactt ccagaccatg cgccgggcga ggttgagcgg
     6961 agtagagggc aagccgcgcc ttcaattcat gacaaggacg cagctcgacg actaccgcta
     7021 ttccaacgac aacacgacgg gccagccggt ttacttcacc atcatcggcg atcagatgga
     7081 gttggtcccg accccgaacg atgattacga cgttgagatc gtctaccgcg ccaacgtgcc
     7141 agcgatcaat tctggcaatt cgtcgaactg gcttctgacc atggcgccgg atctctatct
     7201 gtacggggtc ttgcttgagg cggcgcctta catgaaggca gacgagcgca ttgcggtatg
     7261 ggttgcgggc gtggagtcgg ccatcgatca actcaacaag cacggcgacc ggcagagctt
     7321 cgattccggc ccgaccacta tccgcttgcc gggcgtggtg ccgtaatgtc tctttctcat
     7381 cgaatgagcc gcacgccaga gcataacaca tggtgcgaga ttaggcgccg ctgcgaggac
     7441 ccgaaaagaa acaactatca cacgcatggg ggccgcggca tcaaggtatg tgagaggtgg
     7501 caaaagtttg agaacttcta tgctgacatg ggcgctcgtc cgagtcccaa gcacagcatt
     7561 gatcgaattg acaacgacgg caactacgag cccggcaatt gtcgttgggc tacaaggcga
     7621 gagcaagcgt taaacactag acggaatcta attgtaactg catttggtaa gactgcctca
     7681 ttgggggagt ttttccaaaa tggagcgggt cgcaccagca ctgggtacaa aagggcttgg
     7741 aagcgcatta acaaatatgg ctggggcgct gaacgtgccc tggaggggtg tatttagtga
     7801 cccccttcgg agaatttcgc cctgatgttt cggactatct cggctctgcg acgaagaata
     7861 tcctcaacgt gctgccgcgc ggggacggct atggaccgtt caagtcattc gctgccgcaa
     7921 cccaggcgct tcctgcggca tgccggggag gcttttacgc gctgaagtcg gacggctccg
     7981 tacaggtctt tgcggggaca tcgaagggcc tctatcgcct caacaacacc gaccttagct
     8041 gggtgccggt tggcaaattg gccaattgca ccatttcgaa cgcaagccct ggtgtcgtca
     8101 cctataccaa cacctttgcg gccggcgatc ccgttgtttt tgcggccggg agcgggactc
     8161 ttccggcaga aatcgttgcg gggacggtct actatgttca cgcaacgggc ctgtccggct
     8221 cttcgttcag cgtaagcgca acgtcgggcg ggacgcgcat caacacgtct ggcggctccg
     8281 gcacggtcaa gatcaccgct tactattcgg cgctgtcgag caacgcgcaa tggcagtttg
     8341 cgcagttcgg caatctggtg tttgccacgc aggccaacga agtattgcag gtttataacc
     8401 tgtcctcatc gtcgcagttt gcggataact cgggatcgcc tccgcaggca gcctatatct
     8461 cggttgtcgg tcgatttctt gttctctctg ggctcctttc gaccccgtat cgcgttcact
     8521 ggtccggcct caacgacacg acgaattgga cgagtggtgt tggttcgtcc gactatcagg
     8581 attttcctga cggcggcatt gtacgcggcg tggccggtgg tgaattcggt ctggtcttcc
     8641 aggaccagag catccgccgc atgtcctata tcccgggctc tgcgatcatt ttccagatcg
     8701 agcgcatcac gcaagacctt gggctgttcg cgccgtattc catcgttcgc gccggggcca
     8761 tggtgtattt ccgcgccggg cagggttttt acaagatcgc tcccggagga tatcccgaac
     8821 agatcgggcg cgagcgggtt gaccgcacgt ttcttgaaga cctcgatacg tcaaacctgc
     8881 aattgttcat cggcgccgcc gatcctcgca gttcgcgggt gttctgggcc tacaagtccg
     8941 gcgctggcgc aacagggctc tacgacaagc tgttgggcta tgacgctgct ctcgacaggt
     9001 tcttcccgtt gaccgcgagc ggggaatatc tgaccggcat ctcacaggct ggcattacgc
     9061 ttgaaaacct cgacacgatt tcgtcgtcca tcgacgcgct gaccctgacg ctggactctt
     9121 atgcaagctc ggttcaaccg cagctcgccc aatgggacag caaccacaag cagggcttct
     9181 ttaccgggtc gaacctggag gccacgctag agactgcaga gcagggcacg gacagcaagc
     9241 gcattttcgt aggcggattt cgtcccgtta ccgatgcgcc gacattctac ggctctgcat
     9301 cgttccgtga gaacgtgcag gacgcggtaa cgtcaacgcc tgaagtggcg cgcaacagcc
     9361 gaaccgggcg atgcgacctc aggaagtcca cgcgatattc gaggatgaag gttcgcattc
     9421 ccgcctcaac cgtctggacc tacagtgcgg gtgtagaacc tgatttcagg ccggatggtg
     9481 agacgtgacg atctacgtcc ccggcacgga agaaaaagac ccgaagaagg tcat
//
LOCUS       DAOQEW010000148         9431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2358_length_9431_cov_9.609215, whole genome shotgun sequence.
ACCESSION   DAOQEW010000148 DAOQEW010000000
VERSION     DAOQEW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_9431_cov_9.609215"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..406
                     /gene="rpsJ"
                     /locus_tag="PLS35_14335"
     CDS             95..406
                     /gene="rpsJ"
                     /locus_tag="PLS35_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNI19897.1"
                     /translation="MKVDQRIRIRLRGFDYRVLDQSVVEIVETVRRSGARVVGPIPLP
                     TRIEKFTVQRSTHVDKKSREQFEIRTHKRLLDIMEPTPETMDSLMKLNLAAGVDVEIK
                     L"
     gene            448..1068
                     /gene="rplC"
                     /locus_tag="PLS35_14340"
     CDS             448..1068
                     /gene="rplC"
                     /locus_tag="PLS35_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNI19898.1"
                     /translation="MTNGLLGKKLGMTQIYDESVLEPVTVIEAGPCRVVAIKTKERDG
                     YEAVQLSFGEVKESRLSGGELGHLKKHQAPPSRWLREFPKTGEVAVGATVKVDMFKKG
                     DWVDVVGVSKGKGFQGVVRRHNYAGGPETHGSMFHRAPGSIGASSYPSRVWKNKALPG
                     HMGDERVTVQRLKVIDARPDENLLFVRGAVPGGANGLLVVRKSKKS"
     gene            1072..1695
                     /gene="rplD"
                     /locus_tag="PLS35_14345"
     CDS             1072..1695
                     /gene="rplD"
                     /locus_tag="PLS35_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNI19899.1"
                     /translation="MPIVDVVDSKKKKVGSVDLPDEVFGCKPHGSLVHEAVVMQRACD
                     RQGTASTLRRGEVSGSGKKPWKQKHTGRARAGSLRSPVWRHGGTVFGPKPRSYAFGMP
                     KKKYRAALQSVLSAKLSEGNVVVVSELSIAEAKTKLLANVLAQLGISGTALLVVADPN
                     SHVMQAGKNLSSVTVLRPEELNVYDVLRCHSLVIPQGELERVKEVWS"
     gene            1692..1985
                     /locus_tag="PLS35_14350"
     CDS             1692..1985
                     /locus_tag="PLS35_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNI19900.1"
                     /translation="MKGDLHQVLIQPLLTEKITALREQSNTVGFLVHPDANKIQIKQA
                     VESLLKVKVSRVNVVNIRGKIKRLGRFVGKRSDWKKAFVTLKEGEKLELYETV"
     gene            2018..2845
                     /gene="rplB"
                     /locus_tag="PLS35_14355"
     CDS             2018..2845
                     /gene="rplB"
                     /locus_tag="PLS35_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNI19901.1"
                     /translation="MALKVYKPTTPGRRGMTAIQGEKLTKKKPEKSLTGFHLRTGGRN
                     NDGRLTIRFRGGGHKRLYRQIDFRRDKAGVPAKVAAIEYDPNRSSRIALLHYLDGEKR
                     YILAPVGLSVGDMVQSGEGAEVRPGNALPLVNMPLGTTIHNIELKPGKGGQLIRSAGG
                     SAQVMGRDGAYVQIRLKSGEMRKVLSTCMATVGQVGNVDHENVVVGKAGRTRWKGKRP
                     HVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGTPAKGYKTRKNKSTDKFIIARRKK"
     gene            2855..3142
                     /gene="rpsS"
                     /locus_tag="PLS35_14360"
     CDS             2855..3142
                     /gene="rpsS"
                     /locus_tag="PLS35_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNI19902.1"
                     /translation="MPRSITKGPFVDDHLIKKVEQMNQTKDRKLIKTWSRRSTVIPDM
                     IGHTFAVHNGKKFIPVFVTENMVGHKLGEFAPTRFFKGHGQAKTEKAVALK"
     gene            3187..3540
                     /gene="rplV"
                     /locus_tag="PLS35_14365"
     CDS             3187..3540
                     /gene="rplV"
                     /locus_tag="PLS35_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNI19903.1"
                     /translation="MAEAKANLRFVRVTPRKARVVIDMIRGQQVPMALAMLRHTPKHA
                     ARVIEKLLRSAVANAEQKELGDSDEMWVSQAVVNCGPIYKRFRARSMGRANSIQKRTS
                     HITIAVAAPNAAHSS"
     gene            3573..4241
                     /gene="rpsC"
                     /locus_tag="PLS35_14370"
     CDS             3573..4241
                     /gene="rpsC"
                     /locus_tag="PLS35_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNI19904.1"
                     /translation="MGQKTHPIGYRIGYNYTWSSRWYADKDYARLLHQDIKIRKMVKA
                     KLYHAGVAKVEIERSGDQTRVIIHTARPGIIIGRKGAEVDKLKAALEKEYSGQAYITV
                     KEIKKPELDAQLVSENVATQLEKRVAFRRAMKRSVQSALRLGAQGIKIMVAGRLGGAE
                     IARTEWYREGRVPLHTLRAEVDYGFAEAHTTMGQIGVKTWIYKGELLPALQLKPDSTL
                     GRLG"
     gene            4269..4685
                     /gene="rplP"
                     /locus_tag="PLS35_14375"
     CDS             4269..4685
                     /gene="rplP"
                     /locus_tag="PLS35_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNI19905.1"
                     /translation="MLAPKKVKFRKMQKGRMRGKAYRGGAITLGEFGLKALEPGWITS
                     RQIEAARIAITRFVKRGGQVWTRIFPDKPITKKPAETRMGKGKGNPEYWVAVVKPGRI
                     MYEMGGVAPDVAKQALKLASYKLPIATKFVVRGEFQ"
     gene            4714..4935
                     /gene="rpmC"
                     /locus_tag="PLS35_14380"
     CDS             4714..4935
                     /gene="rpmC"
                     /locus_tag="PLS35_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNI19906.1"
                     /translation="MDVKDLSGLSLEELAAKEKQLRQELFNFRFQLGIGRLENPMQVR
                     STKRDIARLKTVRRQLELLQTQAGQSVGK"
     gene            4950..5219
                     /gene="rpsQ"
                     /locus_tag="PLS35_14385"
     CDS             4950..5219
                     /gene="rpsQ"
                     /locus_tag="PLS35_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNI19907.1"
                     /translation="MKEGQQHRREWYGNVVSNKMNKTVVVAVERSVIHPIYKKVLRRV
                     TKLKAHDEGNVCKVGDRVQLSETRPISKDKHWRVVRVMVKGQPEK"
     gene            5281..5649
                     /gene="rplN"
                     /locus_tag="PLS35_14390"
     CDS             5281..5649
                     /gene="rplN"
                     /locus_tag="PLS35_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNI19908.1"
                     /translation="MIQNYTYMDVADNSGAKQVMCFHVLGGTRRRYGSLGDIVVVAVK
                     EAIPQAGVKKGDVSRAVIVRTTKEVRRDDGSYIKFDRNACVLINAQGEPVGTRIFGPV
                     ARELRWKKFMKIISLAPEVL"
     gene            5676..6002
                     /gene="rplX"
                     /locus_tag="PLS35_14395"
     CDS             5676..6002
                     /gene="rplX"
                     /locus_tag="PLS35_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNI19909.1"
                     /translation="MARIKTKIRKGDTVVVMTGRERGKSGKVLSVDTVTGKVLVEKLN
                     MIKRHTKPNQKLRQGGILEREAPLAISNVMFLCPVTKKPTRLGIKRQEDGRRTRLSKK
                     SKETVE"
     gene            6146..6676
                     /gene="rplE"
                     /locus_tag="PLS35_14400"
     CDS             6146..6676
                     /gene="rplE"
                     /locus_tag="PLS35_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNI19910.1"
                     /translation="MRETYRDQVIPALMKEFGYGNLMQVPRLERIVLNVGMGEAIQNV
                     KLLESAASELGIITGQKPVTTRAKKAIAGFKLRQGMPIGAKVTLRSRRMWEFLDRLIS
                     LALPRIRDFRGISPKAFDGRGNYTLGLKEQLIFPEIEYDSVASIHGMDITIVTTARTN
                     DEGKALLKHLGMPFRA"
     gene            6738..6923
                     /locus_tag="PLS35_14405"
     CDS             6738..6923
                     /locus_tag="PLS35_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNI19911.1"
                     /translation="MSRLALKNKAAVKSKFSCRDYHRCGLCGRVRGFLRRFRMCRICF
                     RSLSLKGEIPGVRKSSW"
     gene            6969..7364
                     /gene="rpsH"
                     /locus_tag="PLS35_14410"
     CDS             6969..7364
                     /gene="rpsH"
                     /locus_tag="PLS35_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNI19912.1"
                     /translation="MITDPIADLLVRLGNAARRRQDVVKVPVSKVKRQILNILGKEGF
                     ILGYGETQEDGHPIFSVQLRYVEEARPMITGMRRISKPGRRVYVGRDDVPKVRNGIGV
                     AVISTSKGLMTDSDSRRAGLGGEVLCAVW"
     gene            7422..7958
                     /gene="rplF"
                     /locus_tag="PLS35_14415"
     CDS             7422..7958
                     /gene="rplF"
                     /locus_tag="PLS35_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNI19913.1"
                     /translation="MSRIGRKPIAVPSGVDVKVAGRLVSVKGPMGKLDWKLTDGLSVA
                     VADGQLVVNRSGDAREIRAMHGLVRAELSNIIQGVTKGYEKSLEITGVGYKAQLQGRE
                     MSFNVGYINPVTYTVPQGIDVKVDKQTLISIKGIDKRLVGQVAANIRSIKPPDVYKQK
                     GIRYAGEVLRKKAGKTGK"
     gene            7968..8333
                     /gene="rplR"
                     /locus_tag="PLS35_14420"
     CDS             7968..8333
                     /gene="rplR"
                     /locus_tag="PLS35_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNI19914.1"
                     /translation="MNTQEKNRKLARRKQRVRKSVVGTSDRPRLNVFRSRSHIYAQII
                     DDLRGHTVAAASTLDESLRKAVKSTGSIEGAKAVGKLIAERAKAANVSMVVFDRGGRQ
                     YHGRVKALADASREGGLQF"
     gene            8402..8893
                     /gene="rpsE"
                     /locus_tag="PLS35_14425"
     CDS             8402..8893
                     /gene="rpsE"
                     /locus_tag="PLS35_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNI19915.1"
                     /translation="MRVNPEELSLKDKVVFINRVAKVVKGGKRFNFCALVVVGDGQGY
                     VGVGKGKAAEVPVAISKAVEQAKKHLVRVPIKGGTIPHEVHGLFGAEHVLLKPAADGT
                     GIIAGGAVRAVVELAGAHNIIAKTLGRGNPFNAVRATLNGLQQLCDPQEIMRLRRAVG
                     SEA"
     gene            8901..9110
                     /gene="rpmD"
                     /locus_tag="PLS35_14430"
     CDS             8901..9110
                     /gene="rpmD"
                     /locus_tag="PLS35_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNI19916.1"
                     /translation="MATEKKTTTAQASLRITLKRSPIGTPYKHRLVLRGLGLRKLNAT
                     VLRPATDQVKGMIAKVGYLLEVSPQ"
     gene            9107..>9431
                     /gene="rplO"
                     /locus_tag="PLS35_14435"
     CDS             9107..>9431
                     /gene="rplO"
                     /locus_tag="PLS35_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNI19917.1"
                     /translation="MKLHDLAPSRGAKHKPKRIGRGPGSGHGKTATKGHKGLKARSGG
                     GKRPGFEGGQMPLIRRVPKYGFTNQFRTEYTIINLKSLAGLETTEAITPQLLMNEGLV
                     RRKGEL"
BASE COUNT         2346 a         2099 c         2880 g         2106 t
ORIGIN      
        1 ccgcgaaggc ggcaagactg tcggctcggg cgtcgttacg gaaattctag cttaggatgt
       61 cctggtctgt tggccggcat gtgaggagtt gagcgtgaaa gtcgatcaaa gaattcgtat
      121 caggttgcgt ggttttgatt atcgcgtgct tgatcagtcg gtggtggaga ttgtggagac
      181 ggtcaggcgt agcggtgcgc gggttgtcgg gcctatccca ttgccgactc gaattgagaa
      241 attcaccgtg cagcgttcga ctcatgtgga taagaagtcc cgcgaacaat ttgagattcg
      301 tacgcacaag cgtctgttgg atatcatgga gcctacgccg gagaccatgg actcattgat
      361 gaagctcaat ttggcggctg gtgtagatgt ggagattaag ctctgagggt ttaaagtctc
      421 gacctttttt gagctgacag gaagaccatg acaaacggat tgttaggtaa aaagttgggg
      481 atgacccaaa tttatgatga gagcgttctg gagccagtga cggtcattga ggctggtccg
      541 tgccgagtgg ttgccattaa gaccaaggag cgcgacggtt acgaagccgt tcagctgtca
      601 ttcggtgaag tgaaggagtc cagactctca ggcggtgaac tgggacattt gaagaagcat
      661 caggctcctc ccagccggtg gctgcgtgaa ttcccgaaaa ctggggaagt cgcagtcggc
      721 gcgaccgtga aggtcgatat gtttaagaag ggtgattggg ttgatgttgt tggtgtttcg
      781 aagggtaagg gctttcaagg tgtcgtgaga cgacacaact atgccggtgg tccagagacg
      841 catggttcca tgttccatcg tgctcctggt tcgatcggcg cgagctccta tccatctcgt
      901 gtctggaaaa ataaggcctt gcctggccat atgggggatg agcgtgtcac ggttcagcgt
      961 ctgaaggtga ttgacgcccg tccagatgaa aacctgttgt tcgtccgcgg agcagtgcct
     1021 ggtggtgcga atgggctgct agttgtgcgg aagtcaaaaa agagttgagc tatgccgatc
     1081 gttgatgtgg ttgattcgaa aaagaagaaa gtcggtagtg tagatctgcc ggacgaagta
     1141 ttcgggtgca agccgcatgg ttcgcttgtg cacgaagccg tggtaatgca gagggcctgc
     1201 gatcgtcagg gcactgcttc cactttgcgt cgcggtgagg tgagcggatc gggaaagaag
     1261 ccttggaagc aaaagcacac tggccgtgct cgagccggtt ctttacggtc ccctgtctgg
     1321 cgacatggcg gtaccgtttt tggtccaaag ccgcgaagct acgcgtttgg aatgccaaag
     1381 aagaagtatc gggctgccct tcagagtgtc ctctcggcca agttgtctga gggcaacgtt
     1441 gttgtagtct ctgagctgtc tatcgcagag gcaaagacaa aattgctggc aaatgtattg
     1501 gctcaactcg gaatttctgg tactgcattg ctcgttgtcg ccgatccgaa ctctcatgtg
     1561 atgcaggctg gcaagaatct ttcaagcgtg accgtgctac gacctgaaga actgaacgtc
     1621 tatgacgtgt tgcgttgcca ctccctcgtc attccgcagg gtgagttgga gcgagtgaaa
     1681 gaggtgtggt catgaaaggc gatcttcatc aggtattgat tcagccattg ctgaccgaga
     1741 aaattacggc cttgcgtgag caaagcaaca cagtgggctt tcttgttcat ccggacgcca
     1801 ataaaattca gattaagcaa gccgttgaat cactcttaaa ggttaaggtc tcgcgtgtga
     1861 atgttgtcaa cattcgcggc aagattaagc gcttaggacg gtttgtcgga aaacgatctg
     1921 attggaagaa ggcatttgtc acgttgaaag agggcgagaa gctcgagctg tacgaaaccg
     1981 tctaagcgcg agtctctcga tagagtgaga tccagttatg gcattaaaag tctataagcc
     2041 aacaacccct gggcgtcgag gaatgacagc catccagggc gagaaactga cgaagaagaa
     2101 gccggaaaag tctttaacgg ggtttcatct tcggactggt ggtcggaata atgacgggcg
     2161 tttgacgatt cggttccgtg ggggagggca caagcgcctc tatcgccaga tcgattttcg
     2221 gcgtgacaag gctggtgttc ccgcaaaggt cgccgccatt gaatacgacc caaatcgctc
     2281 ttcaagaatt gcattgctgc attatcttga tggtgagaaa cggtatatct tggctcctgt
     2341 gggtctgtca gtgggtgata tggtgcagtc aggggaaggc gcagaagtgc ggccggggaa
     2401 tgctctcccg ctcgtcaata tgcctctggg taccaccatt cacaacatcg agctgaagcc
     2461 tggtaaaggt ggtcaattga tccgcagcgc cggaggctcg gcccaggtga tgggtcggga
     2521 tggggcgtat gttcagattc gtctgaagtc tggagagatg cgcaaagtgc tctccacttg
     2581 catggccacg gtcggtcagg tcgggaacgt ggatcatgaa aacgtcgttg tcgggaaggc
     2641 ggggcgtacg cgatggaagg ggaagcgtcc ccatgttcgc ggtgtggtga tgaatcctgt
     2701 tgatcacccg catggaggcg gtgagggaaa atcaggtcaa ggtaacccac atccagtgtc
     2761 cccatggggt actccggcaa agggctataa gacccggaag aataagtcga cggataagtt
     2821 tatcatcgcg cgtaggaaaa agtaggagcg gataatgcct cgttcaatta cgaaaggccc
     2881 atttgtcgat gaccatctga tcaagaaagt tgaacagatg aatcagacga aggaccgcaa
     2941 gttaatcaag acctggtcgc gccgatcaac ggtcattcct gacatgatcg gacacacgtt
     3001 cgctgttcat aacgggaaga agttcattcc cgtgtttgtg actgaaaata tggtcggcca
     3061 taagctggga gaatttgctc caacgcgctt tttcaaaggg cacggacaag caaagaccga
     3121 aaaggcagtg gcgcttaagt aattgtcggt tcactgatga gtcggcatgg tggggtgggg
     3181 tcagctatgg cagaggcaaa agcaaattta cgtttcgtga gagtgacgcc taggaaggcg
     3241 agagtggtta tcgatatgat tcgcggccag caggtgccca tggctttagc catgttgcgg
     3301 catactccaa agcacgccgc ccgagtcatt gagaagcttc ttcgatctgc agtggccaac
     3361 gccgagcaga aggaattggg cgacagtgac gagatgtggg tgtcgcaggc cgtggtgaac
     3421 tgcgggccta tttataagcg ttttagggca cgatcgatgg ggcgagccaa ctccattcag
     3481 aagcggacta gccatattac gatcgcagta gctgctccga acgccgccca cagcagctaa
     3541 cgcttttacc attagactga gggttattct tcatgggtca gaagacacat ccaattggct
     3601 atcgcatcgg ctacaactac acctggagct cgcgctggta tgccgataaa gactatgcgc
     3661 gactgcttca ccaagacatc aagattcgaa agatggtgaa agccaagctg tatcacgccg
     3721 gagtggccaa ggtggaaatt gaacgatctg gggatcagac ccgcgtgatc attcacaccg
     3781 cccgccctgg catcatcatc ggccgaaagg gcgctgaagt tgataagttg aaggctgccc
     3841 tcgagaagga atattccggt caggcctaca ttaccgttaa agaaattaaa aagcccgagt
     3901 tggacgccca attggtgagc gagaatgtcg cgactcagtt ggagaagcgt gtcgcgttcc
     3961 gacgggcgat gaagcgtagc gtgcagtcgg cgcttcggct tggtgcgcag ggaatcaaga
     4021 tcatggtggc gggacgtttg ggcggagcgg aaattgcccg aacggaatgg tatcgcgagg
     4081 gtcgtgttcc tctacatact ttgcgcgccg aggttgatta tgggtttgcg gaagctcata
     4141 cgacgatggg ccagatcggg gtgaagactt ggatctataa gggtgagttg ttgccagcgc
     4201 ttcagttaaa gcctgattct acgctcggac gacttggcta agaacttcga taaggaaaag
     4261 gtgggtctgt gttagcgcca aagaaagtta agttcagaaa gatgcagaag ggccggatgc
     4321 gggggaaggc ctatcgtggc ggagccatta ccctcggaga gttcggctta aaagcccttg
     4381 agcctggctg gattaccagc cgacaaatcg aggcggcccg tattgctatt acccgttttg
     4441 tgaaacgtgg cggccaggta tggactagaa ttttcccgga caagcccatt acgaagaagc
     4501 ccgctgaaac ccgcatgggt aaagggaagg gcaatcccga gtattgggta gccgtggtga
     4561 agcctggacg gatcatgtat gagatgggtg gagtggctcc cgacgtcgcc aagcaggcat
     4621 tgaagcttgc ttcctataag ttgccaattg ccacgaagtt cgtggtgcgc ggcgagtttc
     4681 agtaacctgt agcgagtgtt gagtggtgat gtaatggatg tgaaggatct cagcgggctc
     4741 tcgctagaag agttggcggc aaaggaaaaa caattgcgcc aggaactctt taatttccga
     4801 ttccaactcg gcattggtcg cctcgaaaac ccaatgcagg ttagaagcac gaagcgcgac
     4861 attgcccgcc tgaaaacggt caggcgtcaa ttggaattgc tgcagactca ggccggccag
     4921 tcggtcggaa agtaagagag aaggggcgta tgaaggaagg ccaacaacat cgacgtgagt
     4981 ggtacggcaa cgtcgtcagc aacaagatga ataaaacggt tgtcgtggcg gttgaacgat
     5041 cggtcatcca cccaatttac aagaaggttc ttcgccgggt caccaagctc aaggcccacg
     5101 acgaaggaaa tgtgtgtaag gttggcgatc gcgttcagct gagtgagaca cgtcccatca
     5161 gtaaggacaa gcattggcgc gtcgttcgcg tcatggtcaa gggacaacct gaaaagtaac
     5221 tacggaacac acctgaagag ctgaagggcc tgatcctttt ggcggtgagt agggcggggt
     5281 atgattcaga attacacata catggatgtg gccgataatt ccggcgcgaa gcaggtcatg
     5341 tgctttcatg tgctcggtgg cactcggcgt cggtacggat cgcttgggga catcgtggtg
     5401 gtcgccgtca aggaggcaat tcctcaggcc ggcgtcaaaa agggtgatgt gagtcgagca
     5461 gtcatcgtgc gtacgaccaa ggaagttcgg cgtgacgatg gttcctatat caaatttgat
     5521 cgtaatgcct gcgtcctgat caacgctcaa ggggagcccg ttgggactcg aattttcggc
     5581 ccagtcgctc gtgaacttcg ctggaaaaag ttcatgaaga ttatttcgct tgcgccggaa
     5641 gtcttgtagt cgaaggaaga aaggttctgg tagggatggc acgaatcaaa acgaaaattc
     5701 gcaagggcga caccgttgtg gtgatgactg ggcgtgagcg tggtaagtca ggcaaggtat
     5761 tgtctgtcga taccgtaacg gggaaggttt tggtagagaa actcaatatg atcaagcgcc
     5821 ataccaagcc gaatcaaaaa ctccggcagg gtggcattct tgaacgtgag gctcctcttg
     5881 ccatttccaa tgtcatgttc ttgtgcccag tcacgaagaa gcctactcgg ttagggatca
     5941 agcgccagga agatgggcga cgaaccagac tgagtaaaaa atcaaaagaa accgttgaat
     6001 agtcggaaca ggaaagacgt tcatggcaaa agtagagaag ggtaagggcg gtaagggggc
     6061 aacccctcga gcctccacga agaaggaaac ggcagcgcct gaggtttcac aggaggctgg
     6121 tagtgaacat caatttgcac cgagactgcg ggaaacctat cgtgatcagg tcatccccgc
     6181 gttaatgaaa gaattcggct acggcaattt gatgcaagtg ccacgtcttg agagaattgt
     6241 cctgaatgtc ggcatgggcg aggcaattca aaatgtgaag ttgctggaga gcgcggctag
     6301 cgaattgggc attattaccg ggcagaagcc agtcacgacc agagccaaaa aggctatcgc
     6361 cggttttaaa ttaagacagg ggatgccgat tggcgccaag gtgaccttgc gtagtcgtag
     6421 gatgtgggag tttttggatc gcttgatttc cttagcgctc cctcggatca gagattttcg
     6481 tggtatctcc cccaaggcgt ttgatggtcg aggcaattat acgctgggcc taaaggaaca
     6541 attgattttt ccagagattg aatacgatag tgtcgcatcg attcatggca tggatattac
     6601 cattgtgacc actgcgcgta cgaatgatga aggcaaggcc ctgctcaaac atctcggcat
     6661 gccatttcgg gcctaaaaac agctatcaga gaggttggct cggccttgtg ggccacgcgg
     6721 agtaaagggg gattctcgtg tcaagattag cgctcaagaa taaggcggca gtgaaatcca
     6781 agttctcgtg ccgtgactat caccgctgcg gcttatgcgg tcgcgtgcga ggatttttgc
     6841 gccgatttcg tatgtgccgt atctgtttta gatctttgag cctcaaggga gagattcctg
     6901 gagtgcgtaa gtcaagctgg tagtcgccca ggtgaaagct tgcagttgca ggcatagaag
     6961 aggaatgcat gattactgat ccgattgctg acttgttggt tcgactgggg aacgcggctc
     7021 gccgtcgcca ggatgtcgtg aaagttcccg tgtcgaaagt aaaacgacag atactgaaca
     7081 tccttggcaa agagggattt atcctcggtt acggggagac gcaggaagat gggcatccca
     7141 tcttctctgt ccagctccgc tatgtcgagg aagcgcgccc aatgatcacg gggatgcgtc
     7201 gaatcagcaa gcccgggcgt cgtgtgtatg tcgggagaga tgatgtgcca aaggtgagaa
     7261 acggcatcgg cgtggctgtc atttccacct cgaaaggtct tatgaccgac agtgactcca
     7321 ggcgcgcggg attaggcggt gaagtgcttt gcgctgtctg gtaatgagga gtcgtctagg
     7381 acgcagtata accgagatcg ctgtaacgta acggggttcg tatgtcgagg attgggcgga
     7441 aaccaattgc agttccaagc ggcgtcgatg tgaaggtcgc tggtcgtttg gtatcagtca
     7501 aggggccaat gggcaagctt gattggaagt tgaccgatgg attgagcgtc gcggttgctg
     7561 atgggcagct tgttgtcaat cggtctggtg atgcgcgaga gattcgtgcc atgcacggct
     7621 tggttcgcgc cgagttgagc aatattatcc aaggcgtcac caaaggctat gagaagtcgt
     7681 tagagataac gggcgtgggc tacaaagcgc aacttcaggg ccgtgaaatg agtttcaatg
     7741 tcggctacat caatccggtt acctacacgg tgcctcaggg cattgatgtg aaggtcgaca
     7801 agcaaacact catttcgata aaagggatcg acaagcggtt ggttggtcaa gtagcagcca
     7861 acattcgttc cattaaaccc ccggatgtct ataagcaaaa aggaattcga tacgccggcg
     7921 aagtgctgag gaagaaggcg ggtaaaaccg gcaagtagag gcatttaatg aatacccaag
     7981 aaaaaaaccg aaaattagca cggcgtaaac agcgcgtaag aaaatctgta gtcgggacga
     8041 gtgatcgccc gcggttaaac gtcttccgaa gccgctctca catttatgcc cagatcatcg
     8101 atgatcttcg ggggcatacg gtggccgctg cttctacctt ggatgagtcc ttgcggaagg
     8161 ccgtgaaatc gacaggcagc attgaaggcg ccaaagccgt tggaaaactg attgctgaac
     8221 gagcgaaggc cgccaacgtc tcaatggttg tgtttgaccg tggtggacgg cagtatcatg
     8281 gacgcgtgaa agccttggcc gatgcatccc gcgaaggcgg attacagttt tagaagtgcg
     8341 ttaggcggaa cgcctccgct tcccgataga aaaatcggac gacctaaagg agaatgacgt
     8401 tgtgcgagtc aatcccgaag aattgagcct aaaagacaag gtagtgttta tcaaccgcgt
     8461 tgccaaagtg gtcaaaggcg gaaagcgatt caacttttgc gctttggtgg tcgtcggtga
     8521 tgggcaaggg tatgtgggtg tcggcaaagg aaaagctgcc gaagtcccgg tagctatttc
     8581 caaggctgtt gagcaggcca agaagcacct ggttcgcgtt cccatcaaag ggggaaccat
     8641 tccacacgaa gtccacggac tgtttggtgc cgagcacgtg cttctaaagc cagccgccga
     8701 cggtaccgga atcattgcag gcggagccgt tcgagcggtt gtcgagttgg ccggtgcgca
     8761 caacattatc gccaaaaccc tcggtcgcgg taatccgttt aatgccgtca gggccactct
     8821 taatggactg caacagctct gcgatccgca ggaaatcatg cgactacggc gggctgttgg
     8881 tagtgaagcg taaggacatc atggcgactg aaaagaaaac tacgactgcg caggctagtc
     8941 tgcgaattac cctgaagcgt agccctattg gaacgcccta taagcatcga ttggtactga
     9001 ggggcttggg actgcgcaaa cttaatgcca ctgtgctgcg tcctgccacc gatcaagtga
     9061 agggcatgat tgccaaggta ggctatttac tggaagtgag tccccaatga agttgcatga
     9121 tctggctcct tctcgaggag cgaagcacaa accaaagcga atcggccgtg gccctggttc
     9181 aggccatggt aagacggcga ccaaaggtca taagggtctg aaggctcgtt ccggaggcgg
     9241 aaagcgccca ggatttgagg gcggccagat gccattgatt cgccgcgttc caaagtacgg
     9301 ctttacgaat caatttcgaa ctgaatatac gatcattaat ttaaaaagcc tggctggtct
     9361 ggaaacgaca gaagccatta cgccccaact cttgatgaat gaaggccttg ttcgacgcaa
     9421 aggagagctg a
//
LOCUS       DAOQEW010000149         9281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2426_length_9281_cov_8.740082, whole genome shotgun sequence.
ACCESSION   DAOQEW010000149 DAOQEW010000000
VERSION     DAOQEW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2426_length_9281_cov_8.740082"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1987)
                     /locus_tag="PLS35_14440"
     CDS             complement(<1..1987)
                     /locus_tag="PLS35_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNI19918.1"
                     /translation="MRSVRSGPKKLSQRRSQAAWKLLLGVMLPSVCVGAFQVASAAAP
                     ELHAAAHNQPAWAEQLKGQTIVEDAQEGHVERTAMMERQHQRIMDKINEQLVHDAEVQ
                     RTGGQYNNVNMLHQYGAGNQDLLLMSNSGAEPVSSMGGKCPASAPVRSYDVSAINVEI
                     TLNMWLDFYPGYMYTLTENIEKVRAEETKNKEARDKEGYDPGGVMNGLQNQFIQPLVL
                     RGNQGDCVKLTLRNQLEGDEEVSLNIHGSSMVMSATGQPATTTNPDSIAGKGKAVEME
                     WYIPPTQQEGSRQFHSYSNDRELTVMGLFGSFVIEPKGSEYLEPLGTGDPKPTKSGWQ
                     AIIKNGSGPDFREFVLFYHEVGDEAFRPLNKKGDFIPQRDPLTDVYRPVARALNYRSE
                     PFGVDNMQTQHEYFGFEDESMAYSAYTFGDPATTIPRSYLGDPAKFRLVHGGSEVFHS
                     HHPHGGAIRWPRSPRAIDEMPLWHAAKNGPVKYPVIRTKSDRVDVEVIGPSETMDLET
                     ECGSGLCQQLAGDFLFHCHVAHHYIAGMWGYWRVYNTIQQGANHNDVMPDLLELPDRK
                     GRIKVGVTSDELIGKTVDWFGKQFKIIEKGKTDWKADPAIVTIKDWVDMQFPPQGLPG
                     HKKDERDQTRSYDASVLNWGWQGTRAVTEPENTVPNPR"
     gene            complement(2482..3900)
                     /locus_tag="PLS35_14445"
     CDS             complement(2482..3900)
                     /locus_tag="PLS35_14445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI19919.1"
                     /translation="MGIAGKILIVDDEQDALDNCRRILSRVPYDCLTECDPSRALEII
                     QRERPGLILTDLRMPQLDGIEVLAAAKRMDPSVQVVLLTAHATVQTAVTSMRYGAFDY
                     ITKPFTSRDLVQVARRAFGEDESTGAKQVGATEVRPPVDHGRQGTRATCARVIGESPA
                     MESVFSLIDKVAPTHSNVLVYGESGTGKELVARAIHDASLRADRPFIPVDCVSLPDTL
                     LESELFGHEKGAFTGAHVSKPGLFEVAAGGTVFLDEVSGMSQTLQSRLLRVLQERQMR
                     RVGGIRFLDVDVRVIAASNQDLEAACRRGEFREDLYYRLNVIPIVLPPLRSREGDIPI
                     LAQEFLRRFVKQQGAALGFVPNLDSTATELLCGYAWPGNVRELQNVIERAAVLADGPL
                     ITAAHLPERLRQVVVDEAGPGEAGSFKHAKQAAVTTFERSFLMELLKRNDGHMGRAAR
                     EAGVDRKTVERMVKKHGLRERS"
     gene            complement(3876..5909)
                     /locus_tag="PLS35_14450"
     CDS             complement(3876..5909)
                     /locus_tag="PLS35_14450"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI19920.1"
                     /translation="MTIRTIKGRIVLAIVMVGCIPLLISLVLAYMSGMRSLRDVIGGN
                     FQAIAEQAADRLTMLVQSEVQGVRLLASAPLRVRQPVEAANLSYKGEWADTQRLIQER
                     AKEWEKGHDNAAGLLNLELSRFLLETKVRNGDKMVGLLITDRYGALVAASSEPDHYFL
                     SQEPWWEALQAGGLDRVYVSGLIPGQEGSFRSPEETIDIAVPILDDHQHAVIGAIKAS
                     YRFDSLFAMIKEIRIGQTGHAMLFDAAGQPLVCPILPRRAHRIPGQLMAMIVSSNPGW
                     GIAEDDGHGATDTVVGYAPVTGLSLPDNSWHVFVRQQPEETYAPIRDQLRNLALIGVV
                     MSGLLWAMGRYVATRIARPIQVLKTGVEAISRGTYDGPLDVKTGDEFEELAVAVHRMA
                     DRLQASRIELESLNAELTHRVEEKTAEITRQMRKLELSERLATLGKVASGIAHEINNP
                     LGIILNRIECMEADAAQSNVPDEVGRDLVTIRTQAERISRVTRSILTFSRGTVSMLKP
                     LDLNCVARTCVAMAGERMTGLAVRMDLETAAALSPVMGDRDRLETVLLNVINNAIDAV
                     TASGEQGVVTIRTARVQINGDEWVQISISDTGPGVPSAIADRIFDPFFTTKPAGQGTG
                     LGLFLSYGIVSDHRGRIEVRNDSVGAVFDIYLPAVGLGESVHEGAPWGLQEKF"
     gene            complement(5983..6789)
                     /locus_tag="PLS35_14455"
     CDS             complement(5983..6789)
                     /locus_tag="PLS35_14455"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNI19921.1"
                     /translation="MSRGGSERIVRLAHRWLGMGSLGFLLLSVVTGLLWADAKFLYWD
                     DHYKEKVRPAANPGIEAARLSVDRAILAAKEAVGGRALVEHVLLRSDFGRVMYELKLR
                     VEGVPTTLLLDAINGERLSPISPDLAPVIAQQYVRTPAAVTGVELERYTPRKKKRAQD
                     AVRVRFDNADATEIVLDRQTGEILEDEGRWRRVHFAVMQLHQLNFFGFEKTLLNIPGV
                     PILLMSLSGCLLWIFHRARMRRAQLAPVSRETGLFPARSTGEPKEVPPLG"
     gene            complement(6791..7600)
                     /locus_tag="PLS35_14460"
     CDS             complement(6791..7600)
                     /locus_tag="PLS35_14460"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNI19922.1"
                     /translation="MNRNLFNSGLWGIVLVAGLWGAVPAQAHFVWVETEATAPADQGQ
                     PVKVYFGEYAEFLREERGGRLDSIDGVTVRVQDPKRGKIDVPLTKQVNHFAGALSSCV
                     PGRNAVVAEQAKAPVQDLRKHDLGIVKPMFYARTYFVCLEESRVNEHERDHSAPLALD
                     LIPLTTGLNLAAGRVTPGPGGEIVVKAIFNGQRLPGTQVLVHAPNGWDKELKTDADGI
                     VSFTPLWPGRYVLEMIHVEKTPGEFEGTAYEALRHRSTLALQVRAERPAEQ"
     gene            complement(7647..>9281)
                     /locus_tag="PLS35_14465"
     CDS             complement(7647..>9281)
                     /locus_tag="PLS35_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI19923.1"
                     /translation="GASVLYSPNTQGGLINFIQKRIPMTPTFSTTNTYGSFNLFQSDT
                     QYGGYWGNFGAQLGYLRRQSDGFRDRSKSQLDDFTMRFEANPDDRTRITTNISWYDET
                     TQTPGSITPTQYRNDRTLAGKPNDEFKGQRGAIDVTASREIDAHNTAKVIVYGNVFER
                     NWYIQDQAANGTLLSTSTNFLRKFNVFGVMPQHQFKFSLFGLDQQITSGVRYHAERLT
                     DITGIGTAGSKISRTTNNADLESVAYAAYTETAIRLTEALTISPGIRWEQVNQSRETM
                     NAVPPAGGNFKGYKTTQGLIYGTGVKYQLTPDSMLFGHVHTTFRPPTFANAVDPTSGT
                     TQDLSAERALSSDVGIRSILVPGVSAEVALFRTEFSNQIVQQGSQFVNAGKTLMEGIE
                     STVSLDWGEIVRPLQGFVTRGSITLLRPKSLFGATDGKDLPYAPRMTFYGLVGYYHPT
                     GASIEADAMYVAQQFTDGANTQAENALGSNGAIPSYTIWNLRLNYAPPKAKWAIFAGV
                     RNLTDESFIAQRTTGSFIGIQPGEIRNFYGGVSWRF"
BASE COUNT         1904 a         2958 c         2516 g         1903 t
ORIGIN      
        1 accgcggatt cggcaccgta ttctcaggct ccgtcaccgc acgagtaccc tgccaccccc
       61 aattcagcac gctggcgtca taggagcgtg tctgatcgcg ctcgtccttt ttgtggccag
      121 gcagaccctg cggcgggaat tgcatatcga cccagtcctt gatcgtcacg atggccgggt
      181 cggccttcca gtcggtcttg cccttctcga tgatcttgaa ctgcttaccg aaccaatcga
      241 cagtcttgcc gatcagctcg tctgacgtca cacccacctt gatgcggccc ttgcggtccg
      301 gcaactccag cagatccggc atcacgtcat tgtgattggc accctgctga atggtgttgt
      361 acacgcgcca gtagccccac atgcccgcga tgtagtgatg cgccacgtgg cagtggaaca
      421 ggaagtctcc ggccaactgc tggcagagac cggatccgca ctccgtttcc agatccatgg
      481 tctcggacgg tccgatgact tccacgtcga cgcgatcgga cttcgtccgg atcacgggat
      541 atttcacggg accgttcttc gccgcatgcc acaggggcat ttcgtcgatg gcccgcgggc
      601 tccgcggcca acgaatcgcc ccgccatgcg gatggtgcga gtgaaatact tcggagccac
      661 cgtgcaccag ccggaacttg gccgggtcgc cgaggtagct gcgcggaatg gtcgtggccg
      721 gatcgccgaa cgtataggcg ctgtaggcca tcgactcgtc ctcgaagccg aagtattcgt
      781 gctgcgtctg catattgtcg acgccgaacg gctcgctccg atagttcaag gcacgggcca
      841 ccggacgata gacgtcggtg agcggatcgc gctgcgggat gaagtcgccc ttcttgttca
      901 atgggcggaa cgcttcgtcg ccgacttcat gatagaacag cacgaactcc cggaagtccg
      961 ggccgctgcc gttcttgatg atcgcctgcc agccgctctt ggtcggcttg ggatcgccgg
     1021 ttcccagcgg ctcgaggtat tcagatccct tcggctcgat cacgaaggaa ccgaagaggc
     1081 ccatgacggt caactcacga tcgttgctat acgaatggaa ctggcggctg ccctcctgct
     1141 gcgtcggggg gatataccat tccatctcca ccgctttgcc cttgcccgca atgctgtcgg
     1201 ggttggtggt cgtggccggc tgtcccgtcg ccgacatgac catgctggaa ccatggatgt
     1261 tcaagctgac ttcttcgtca ccttccagct gattgcggag cgtcagcttc acgcaatcgc
     1321 cttgattgcc gcgaaggacc aatggctgaa tgaactggtt ctgcaatccg ttcatgacgc
     1381 cgcccggatc gtacccttcc ttgtcacgcg cttctttgtt cttggtctct tcggcacgaa
     1441 ccttctcaat gttctcggtc agcgtgtaca tgtagcccgg atagaaatcg agccacatat
     1501 tcaaggtgat ttccacattg atcgccgaca cgtcgtagct gcgaaccggc gcgctggctg
     1561 ggcacttccc gcccatcgag gagacgggct cggcgccgct gttcgacatc aataacaaat
     1621 cctggttacc ggcgccgtac tggtgcaaca tgttgacgtt gttgtactgg ccgcccgtcc
     1681 gctgcacttc agcatcatgg accaactgct cgttgatctt atccatgatc cgctggtgct
     1741 ggcgctccat cattgcggtg cgctcgacat ggccttcctg cgcatcctcg acaatggtct
     1801 gccccttcaa ttgctcagcc catgcaggct gattatgcgc ggccgcatgc agctccggcg
     1861 ccgccgccga ggccacctgg aacgccccga cacagaccga tggaagcatc acccctaaga
     1921 gaagcttcca cgccgcttgg gacctgcgct gactgagttt cttaggcccg ctacgtaccg
     1981 atctcattag acctacctcc tcttgaaacc atacaacctc cgcatcccca ctctcttgcc
     2041 atcgcactcc ggtcgcgcac ggtgcaggcc cgtgaagacc tgcacctcat ggagtggaaa
     2101 caccaggagg ccggtccttc ggtttccaca cctttgatcc gcgataacaa gacgatggcg
     2161 cacttcacaa cgcgcatgaa aagcaatgcg catgaaaaga cggcgagtta cagagcaact
     2221 cccgttccag attcattggg tccaggaaac gcagactttg ttgggaattt cagacagtga
     2281 agatgaagga gagaataaaa atggggcgac gtcgccccat caaaaaacca caattctcag
     2341 gaatgctgcg aactgaaagg cgaacggtga gtgtccggaa catttgtccc aatgggacat
     2401 tgatgcccca attgaaaact gagggggaaa ccccgaatca ggcatcgtga ataaccacgg
     2461 tacgccgtga cagaaaacgg atcaggagcg ctcgcggagc ccatgcttct tgaccatgcg
     2521 ctcgaccgtt tttcgatcca ccccggcttc ccgagcggct cgccccatat ggccatcgtt
     2581 tcgcttgagc agttccatca gaaaactccg ctcgaaggtc gtgaccgccg cttgcttcgc
     2641 atgcttgaag gagccagcct ctcccggtcc tgcctcgtcc accaccacct ggcgcaaccg
     2701 ctcgggcaaa tgcgcggcgg taatcagcgg accatcggcc agcacggcag ctcgttcaat
     2761 gacgttttgc aattcgcgca cattccccgg ccaggcatac ccgcacagca actccgtcgc
     2821 cgtggagtcc aggttgggga cgaacccgag ggcggccccc tgctgcttca cgaatctccg
     2881 caaaaattcc tgggccagaa tcggaatatc accctcccgg ctccgcaacg gcggcaacac
     2941 gatgggaatg acattcaggc gatagtagag gtcctcgcga aactctccgc gccggcaggc
     3001 ggcctcgaga tcctggtttg atgcggcaat cacccgcacg tcgacatcca ggaaccggat
     3061 gcccccgacc cgccgcatct ggcgctcctg aagaacccgc agcaatcgcg actggagggt
     3121 ctggctcatc ccgctgacct cgtccagaaa gacggtcccg cccgccgcca cttcgaacag
     3181 cccgggcttc gagacgtgtg cgccggtgaa ggcgcccttc tcgtggccga acagttccga
     3241 ttcaaggagc gtatcaggca acgagacgca gtccaccgga atgaaggggc gatcggcccg
     3301 caaactggcg tcgtgaatcg cgcgcgcgac aagttctttt cccgtcccgc tttccccgta
     3361 gaccagcacg ttggaatgcg tcggcgccac tttgtcaatc aggctgaaga ccgattccat
     3421 ggctggactc tcaccaatga cccgtgcaca agtggcccgc gttccctgac gcccgtgatc
     3481 gacgggaggt cgaacctcgg ttgccccgac ttgcttcgca ccagtcgact catcctcacc
     3541 gaatgcgcgg cgagccactt ggacaaggtc gcgactggta aatggcttgg ttatgtagtc
     3601 gaaggcgcca tagcgcattg acgtcaccgc ggtctgcacg gtcgcatgcg ccgtcaacaa
     3661 taccacttgg acagagggat ccattcgctt agctgcagcc agcacttcga tgccgtccag
     3721 ttgtggcatt cgcaaatccg tcaggatcaa cccgggccgc tcgcgctgaa tgatttccag
     3781 cgcccgcgac ggatcacact cagtcaggca gtcatacggc acacggctga gaatccgcct
     3841 gcaattgtcc aacgcatcct gctcgtcatc gacgatcaga atttttcctg caatccccat
     3901 ggtgctcctt cgtgcacgct ctcgcccagt cccaccgcag gcagatagat gtcaaagacg
     3961 gcgcccacgc tatcgttccg cacctcgatg cgcccgcgat gatccgagac gatgccataa
     4021 ctcaaaaata gccccaagcc ggttccctgg ccggccggct tggtcgtaaa aaacggatca
     4081 aaaatccgat cagcaatcgc cgagggcacc ccaggtccag tgtccgatat gctgatctgc
     4141 acccactcat ctccattgat ctgcacgcga gcggtacgga tcgtgaccac tccctgctcg
     4201 ccagatgctg tcactgcgtc aatcgcatta ttgatcacgt tcagcaacac ggtttccaat
     4261 cgatcacgat cacccatcac cggcgacaac gccgcggccg tttccaagtc catccgcaca
     4321 gcaagacccg tcattcgttc gcctgccatg gccacgcacg ttcgtgccac gcaattcaag
     4381 tcgagcggtt tcagcatgga caccgtacct cgggaaaacg tcagaatgct tcgcgtgacg
     4441 cgggaaattc gttctgcctg ggtccggatc gtgacgagat cgcgaccgac ctcatccggg
     4501 acgttcgact gggcggcatc agcttccata cattcgatgc gattgagaat gatgccgagt
     4561 ggattgttga tctcatgcgc gattccgctg gccactttgc ctaacgtcgc caaccgttcc
     4621 gacagttcca attttcgcat ctgccgtgta atctccgccg tcttctcttc cacccgatgt
     4681 gtgagttcgg cattcagcga ctccagttcg atacgcgacg cctgcaatcg atccgccatg
     4741 cgatgcaccg cgacggccag ttcctcgaat tcatcaccgg ttttgacgtc gagcggccca
     4801 tcgtaggtgc cacgactgat cgcctccacg cccgtcttga gcacttgaat cggccgggca
     4861 atcctggtgg cgacgtaccg ccccatcgcc cagagcaagc ccgacatcac gacaccgatc
     4921 agggcgagat ttcgtaactg gtcgcgaatg ggcgcgtagg tttcctccgg ctgttgccgc
     4981 acgaagacgt gccaggagtt gtccggcaga ctcaaacccg tgacaggcgc ataccccacg
     5041 accgtgtcgg tcgcgccatg gccgtcgtcc tcggcgatgc cccagcctgg gttcgacgag
     5101 acgatcatcg ccatcaactg gcccggaatc cgatgtgcgc gacgaggcaa gatagggcac
     5161 actaacggct ggccggcggc atcgaacaac atggcatgcc ccgtctgccc gatgcggatc
     5221 tctttgatca ttgcaaacag agagtcgaac cgataagagg ccttaatcgc accgatgacc
     5281 gcgtgctgat ggtcgtcaag aatgggaacc gcgatgtcga tggtctcttc aggcgagcga
     5341 aacgatcctt cctggccggg aattagcccg ctgacataca cacggtcgag gcctccggct
     5401 tgtaaggcct cccaccacgg ttcctggctg aggaagtaat gatccggttc ggaactggct
     5461 gcgaccaatg ccccataacg gtcggtgatc agcaacccca ccatcttgtc gccgttacgg
     5521 actttcgtct cgagaagaaa tcgagacagt tccaaattta ataggcccgc agcgttgtcg
     5581 tgtcctttct cccactcctt cgcgcgttcc tgaatcagcc gctgcgtatc ggcccactcc
     5641 cctttgtagg acagattggc ggcttcgacc ggctgacgga cacgtaacgg cgcggacgca
     5701 agcaaccgca ctccctgcac ttcgctctgc acgagcatcg tcagacgatc ggcagcctgc
     5761 tcggcgatcg cttgaaaatt cccgccgatg acgtcgcgaa gggagcgcat gccggacatg
     5821 taggccagaa cgaggctgat caggagcgga atgcagccca ccatcacgat ggcgagcact
     5881 atgcgaccct tgatggtacg aatcgtcatt ccccacctca gtgaggacat tctaaacaac
     5941 cggcggaaga aaacgccacc gcagggtcaa cccggccgat gatcaaccca ggggtggcac
     6001 ctcctttggt tcgcccgtcg aacgggcagg aaacaacccc gtctccctgc tgacgggggc
     6061 caattgcgct cgccgcatcc tggcacgatg aaagatccat aacaggcatc ccgagagact
     6121 catcaagaga atcggcacgc cgggaatgtt caagagagtc ttttcaaagc caaagaaatt
     6181 cagctgatgc aattgcatca cggcaaaatg cacgcgccgc cagcgtcctt cgtcttccag
     6241 gatctcgcct gtctgccgat ccagcacaat ctcagtggca tcggcattat cgaatcgcac
     6301 ccgcaccgca tcctgtgccc gctttttctt gcgcggggta taccgttcga gttccacgcc
     6361 cgtcacggct gccggggtcc gtacatactg ctgtgcaatc accggtgcca gatccggtga
     6421 aatgggagac aggcgctcac cgttgatggc atcgagcaag agcgtggtcg gcaccccctc
     6481 gacccgcaat ttgagttcat acatcacgcg gccaaaatcc gatcgcagca acacgtgctc
     6541 gactaatgcc cgtcctccca cagcctcctt ggcggcgaga atcgcacgat ccacggatag
     6601 cctcgccgct tctatgccag ggttcgccgc tggccggacc ttctccttat aatggtcatc
     6661 ccaatagaga aacttcgcgt ccgcccacaa caggccggta acgaccgaga gcaacagaaa
     6721 gcccagggag cccatgccaa gccagcgatg agccagcctc acaatccgtt cagacccgcc
     6781 gcgactcatg ttattgctcc gccgggcgct cggccctcac ctgaagcgcc agcgtgctgc
     6841 gatggcgcaa ggcctcatac gccgttccct cgaactcgcc cggagttttt tcgacatgaa
     6901 tcatctccaa cacgtaccgg cccggccaca gcggggtgaa ggacacaatc ccgtcagcat
     6961 ccgttttgag ctccttgtcc cagccattgg gcgcatgcac cagcacctgc gtccccggca
     7021 ggcgttgccc gttgaagatc gctttcacca cgatctcgcc cccgggaccg ggagtgacgc
     7081 ggcccgccgc caaattcagc cccgtcgtga gcgggatcag atccaaggcc aacggcgccg
     7141 agtggtcacg ttcatgctcg ttgacccggc tttcttccag gcagacgaaa taggtccgcg
     7201 catagaacat cggcttgacg atgcccaggt catgcttgcg cagatcctgc accggcgctt
     7261 tcgcctgctc cgcgaccacc gcgttccgcc ccggtacaca ggatgacagg gctccagcaa
     7321 agtggttcac ctgcttcgtc aacgggacat cgatcttccc tcgcttcgga tcctgcacac
     7381 gcacagtgac gccatcaatg gaatcgagcc gtcccccacg ttcttcccgg agaaattctg
     7441 catactcgcc gaaatacact ttcaccggtt gcccctggtc cgcgggagcc gtcgcttcgg
     7501 tttccaccca gacgaaatga gcctgagccg gaacagcgcc ccacagcccg gccaccaata
     7561 cgattcccca caatccacta ttgaataggt tccgattcat cagcgtcatt cctttctgaa
     7621 gttcgctcaa aggttgcgac ttcccatcaa aatctccagg agaccccgcc atagaagttc
     7681 cggatttcac cgggctggat gccgataaaa ctgccggtgg tacgttgggc gatgaatgat
     7741 tcatccgtca ggttgcgcac accggcgaaa atggcccact tcgccttcgg cggagcataa
     7801 ttgagccgga gattccaaat cgtataggaa gggatggcac cgttcgaacc caaggcattt
     7861 tccgcttggg tattcgcgcc atcggtaaat tgctgtgcga catacatggc atcggcttca
     7921 atcgatgcac cggtcgggtg gtaatatccc accaagccgt agaacgtcat gcggggggcg
     7981 tacggaaggt ctttcccgtc cgtcgctccg aagagggact tcggccgtaa gagagtgatg
     8041 ctccctctgg tgacgaagcc ctggagcggg cggacgattt caccccaatc gagactgaca
     8101 gtgctctcga ttccctccat cagcgttttt ccggcattca cgaactggct gccctgctga
     8161 acaatctgat tactgaattc ggtgcgaaac aacgcgactt ccgcactcac cccgggcacc
     8221 aggatggacc ggattccgac atcggaactc aacgcgcgtt ctgcggagag atcttgggtg
     8281 gtgccgctcg tcggatcgac cgcattggcg aaggtcggcg ggcggaaggt cgtatgaaca
     8341 tggccaaaca gcatgctgtc cggggtgagt tgatacttga cgccggtccc gtaaatcagt
     8401 ccttgcgtcg tcttgtaacc cttgaaattc cctcccgccg gcggaacggc gttcatcgtc
     8461 tcacgacttt gattgacctg ctcccagcgt atgcccggcg aaatggtgag ggcctcggtc
     8521 agacgaatcg ccgtttccgt ataggcggca taggccaccg attccagatc ggcattgttc
     8581 gtcgtgcgac tgatctttga gccggctgtc ccgataccgg tgatgtcggt cagtcgttcg
     8641 gcgtgatacc gaacaccgga cgtgatctgc tgatccaatc caaagagcga aaatttgaac
     8701 tgatgttgcg gcatgacgcc gaagacattg aacttgcgca aaaagttcgt gctggtggag
     8761 agcagcgtgc cgttggcagc ctggtcctgg atataccagt tccgctcgaa gacattgccg
     8821 tacacgatga ccttggcggt gttgtgagcg tcgatttccc ggctggccgt cacgtcgatc
     8881 gctccccgct gccctttgaa ttcatcgttc ggtttgcctg ccagcgtacg atcgtttcgg
     8941 tattgcgtgg gcgtgatgct tccaggggtc tgcgtcgttt cgtcgtacca tgaaatattc
     9001 gtcgtaatcc gcgtccgatc gtcaggattg gcttcgaatc gcatcgtgaa atcatctaac
     9061 tgcgatttgc ttcgatctcg aaatccgtct gactggcgcc ggagataccc gagctgcgcg
     9121 ccaaaattcc cccaatagcc cccgtactga gtgtccgatt gaaataggtt gaaactgccg
     9181 tacgtattgg tggtagaaaa cgtcggtgtc atgggaatcc gcttctggat gaaattaatc
     9241 aggcctccct gcgtgttcgg tgaatagagc acggacgcgc c
//
LOCUS       DAOQEW010000150         9019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2554_length_9019_cov_8.605422, whole genome shotgun sequence.
ACCESSION   DAOQEW010000150 DAOQEW010000000
VERSION     DAOQEW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_9019_cov_8.605422"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="fliS"
                     /locus_tag="PLS35_14470"
     CDS             <1..317
                     /gene="fliS"
                     /locus_tag="PLS35_14470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNI19924.1"
                     /translation="LYDSAIQSMELAREAILTNNYKDKARFLDRSMAIVGELSSVLDF
                     ERGGEIAVSLHRLYDYMVQQCIQANLRHNGKHLDGPIRCLTTLREGWQVVARQEAVAH
                     VA"
     gene            307..648
                     /locus_tag="PLS35_14475"
     CDS             307..648
                     /locus_tag="PLS35_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19925.1"
                     /translation="MSPSSSHPGSPHDRRSIEQLTIQALNAARAGDWDQVDACYSERG
                     LSLAACKPDHTVAQQLLIMDEEIRQAILLAQAGISALLAEASHVTRHLRQLRESSGQL
                     ASDSVTIHREA"
     gene            706..1326
                     /locus_tag="PLS35_14480"
     CDS             706..1326
                     /locus_tag="PLS35_14480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI19926.1"
                     /translation="MRPSPERPLQRPHLDINNEERREWLRIDDRLLLEYRRADEPLEA
                     MNQYLPPATEDTIATAVSKPTVDLLVRAGETFAGSPLLPWVSKIDWMLETILKSMVKS
                     HPGSVSIARLTDVDISAGGLGFNTPREFQADDLLMLKVILPPFSMIETMAKIIRVTPL
                     DEGKAGFHLATQFVDLGGDEQELIIRHILQIQAERLRTRKAAHNHL"
     gene            complement(1357..2286)
                     /locus_tag="PLS35_14485"
     CDS             complement(1357..2286)
                     /locus_tag="PLS35_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNI19927.1"
                     /translation="MATIVSIGSGKGGVGKSVIAANLAMLLAKRGKRVVLADLDVGGA
                     DAHILFGLLNPPRTLTDFVERRVERLEEVLQPISAHPFLQLLPGTGDTLATANLPYAK
                     KKRLIRHFSQLQADVIIVDIGAGTSYHALDFFLMADCYLTVATPDPTSVLDLYRFIKL
                     AAIRRVLSAFLSRDAVSEALSDRDFASIDEVIQAVGETDPNAKETASNTLQGFQPYLI
                     VNRVSGKSRVNVLQLKKLLKEYVGGDLTMLGEIPDDPAMVRAVRSYLPVVEFEPTAPA
                     SVALNGAADALLAQLTKPATMPQPSAPSPERAA"
     gene            2479..2709
                     /locus_tag="PLS35_14490"
     CDS             2479..2709
                     /locus_tag="PLS35_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI19928.1"
                     /translation="MNNAIAPTTNVNFDSVSPSEGEILTVSDVARFLRVPKSTVYKLA
                     RLGELPASKIGKHWRFLRRDIHEWMHSRTQQH"
     gene            2815..3678
                     /locus_tag="PLS35_14495"
     CDS             2815..3678
                     /locus_tag="PLS35_14495"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNI19929.1"
                     /translation="MAESNAVRKTEMPAPKLSPDTFKQLRDLIYEMTGIHFQDNKTYL
                     LESRLLPRLKACRCQTFESYLNFLRFDAYRDREITELYTVITTNETYFFRDEAQLDSF
                     MKVMIPEVMKTNAQTKQLRIWSAACSTGDEPYTLALLLRDYPPLNGWTIDILATDISE
                     NVLNIARTATYSSHSLRKVPPAMLARYFTGKKEEQTLVQPVKDMVRFMNVNLYDRPRL
                     KLIRGIDIIFCRNCLIYFDEKAKAQIVSDLRDALRPKGYLMIGFSETLHDAAGHFKTI
                     HAGRSVIHEKQ"
     gene            3688..4068
                     /gene="cheY"
                     /locus_tag="PLS35_14500"
     CDS             3688..4068
                     /gene="cheY"
                     /locus_tag="PLS35_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNI19930.1"
                     /translation="MPADPNMKILVVDDMSTMRRIVKNILKQLGFNNLEEAENGQEAL
                     TKLKADTYGFVVSDWNMPVMMGIDMLRAIRADESLKKIPVLMVTAEAQKENLMEAVQA
                     GVSNYVVKPFTAETMQDKINKIFK"
     gene            4095..4745
                     /locus_tag="PLS35_14505"
     CDS             4095..4745
                     /locus_tag="PLS35_14505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNI19931.1"
                     /translation="MAQPRAAAQSQPATHESEQEEKAPDTKLYEELGELARFIDTTMK
                     TLSEFSTPVSTSTEQLPQALSHLTNLKTMTEQGTHRVMLEVEAIQDNHMKISGMIKDL
                     TLAMQKAQAAPALLQQMQAVGNAINVDDKRLLDIMTALSFQDLVAQSVNKLVTILQEV
                     EHKLLQLVVVFGPYQKQAAKKEQDKASEMLKQLEATKNTSMNQDLADEILKQFGFN"
     gene            4754..6646
                     /locus_tag="PLS35_14510"
     CDS             4754..6646
                     /locus_tag="PLS35_14510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNI19932.1"
                     /translation="MSDEMQEILNDFLTESNEMLEVLDQRFVTLESDPNNTDLLNEIF
                     RAMHSMKGSAGFLGFNHLVDVAHRGENILNKLRQGEMAVNPAIISIILETIDVIKAIM
                     ADIRESGTDNHVATASIAAKLDDILNGNTAPAAAPVQTEAVAPPKVEAASSAPAAAAP
                     VAAPQTLGEILVNDGLASKEQVLDALTAQQHQPEPKTPLGEILLQAKAITERALDQAL
                     HKQDKQPKPVEEDATIRVETKRLDSVMNLVGELVLGRNRLIKIGTQLEQQHESDPQVR
                     VLSETLAQLNLVTTDLQLAVMKTRMLPIKKVFAKLPRMVRDLSQKLNKQVHLEMRGEE
                     TELDKSVADEIGDPLVHLVRNAIDHGIETPAERQAKGKAGEGQLTIAASQEGNSIVIR
                     INDDGRGIQVEKVKEKALAKGLISEAELATMEHREILNLIFLPGFSTAEKVTDVSGRG
                     VGMDVVRTNIRKINGSVDLESEPGKGSQIIIKLPLTIAIIQALMVEVERSIFAIPLST
                     VIEAVRISRSEIKTINGREVLHLRDRVLPLIRLAQEFDIPTDANRERFYVVVAALGDR
                     RVGVVVDELRSQEEVVIKSIWDYLETVKGVSGATITGEGKVVLILDTSELVQNAQAWH
                     NPGIAA"
     gene            6732..7715
                     /locus_tag="PLS35_14515"
     CDS             6732..7715
                     /locus_tag="PLS35_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNI19933.1"
                     /translation="MSTLINEIDARTRLAGANQMELLLFKLGTNEIFGINVFKVREVM
                     KLPELTQIPEADSRIVGMANIRGIMVPVVGLKRSLGLGMETEGQGNGAPGGNPYLIVS
                     EYNGSLQGFLVAGVDRIIRFSWSAIKTPPAIVRENNKGAVTAVTMLEDGRMVLILDVE
                     KVLNDICPRTDDDVFAGMTIAPELRSKCVLFADDSSVARMQIRKALDRLSMSYVIATT
                     GGEAWQKLQALADQATAEGKEQVDQIQCILSDIEMPEMDGFTLTKHIRADPRLAHLPV
                     MLHSSLTGACNMEKGRTVGATDYITKFDAKMLGEKLALYIQNPNANVKKAA"
     gene            7796..8893
                     /locus_tag="PLS35_14520"
     CDS             7796..8893
                     /locus_tag="PLS35_14520"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNI19934.1"
                     /translation="MALPLTQPQSAAKPVRVLVVDDSAFMRKSLTTMLEEGKQIQVVG
                     VARNGEEAVQQVQQLKPDVVTMDVEMPGMTGLQALQQIMTKNPVPVIMVSSVTVEGAQ
                     ETLQALEWGAVDFIPKQLDGVASKIAEIQKILVPKVLAARYAGSKLKRLNVTGAPKLA
                     VPAAKALSSRSVSVTRGTKLIAIGCSTGGPQALFEIVPTIPADCPAGIVIVQHMPSSF
                     TKPFAERLNNLCALEVREAVDGDEVKAGRVLVAPGGMQFRVVKKSITTSVVKLSPNYE
                     RHAHSPSVDIMLQSVAALFGERSIGVILTGMGHDGLEGMKAIKAAGGRTVAQDEASSV
                     VYGMPKAVVEAGCAEKVVSLSKVIGEIMNMV"
BASE COUNT         2182 a         2516 c         2581 g         1740 t
ORIGIN      
        1 tgctctacga ttctgcgatt caatcgatgg agctcgcccg ggaagccatt ctcacgaaca
       61 actacaagga taaggcccga ttcctcgatc gcagcatggc catcgtcgga gaattgtcca
      121 gcgtcctgga tttcgagcga ggcggggaga tcgccgtgtc cctgcaccgg ctgtatgact
      181 atatggtcca gcaatgcata caagcgaatc ttcggcataa cggcaaacac ttggacggac
      241 cgattcgctg cctgacgacc ctccgggaag gatggcaagt cgtcgcacga caagaagcgg
      301 tggcgcatgt cgcctagttc ctcccatccc ggttcccctc acgaccgtcg ctccatcgaa
      361 caactcacca tccaagcact gaacgccgcc cgagcagggg actgggatca agtcgacgcc
      421 tgttatagcg agcgcggact cagtcttgcc gcatgcaaac ctgatcacac cgttgcacaa
      481 cagcttctca tcatggatga agaaattcgc caggccatcc tgctcgcgca agctggaatc
      541 tccgctctct tggctgaggc ctctcacgtg acccgccacc tgcgccaatt acgcgaaagt
      601 agcggacaac tggcctcaga cagtgtaacg atacatcgag aagcctagcg ttcttgcagc
      661 cagataccct ggcttccccg tgaccttcca ctagagaggt ttggtatgag gccttcacct
      721 gagcggccgc tgcagcgccc gcatctcgac atcaataatg aagagcggcg cgagtggctc
      781 cgcatcgacg atcgattgct gctcgaatat cgacgagccg acgagccgct ggaggcgatg
      841 aatcaatatt tgccgccggc gacggaagac accattgcta cggccgtatc caagccaacc
      901 gttgatctgc tggtacgggc aggggagacg tttgcgggtt ctccgctcct gccgtgggta
      961 tcaaaaatcg actggatgct ggagacgatc ctcaagtcga tggtcaagag tcacccgggc
     1021 agtgtgagca tcgcgcgact gaccgatgtg gatattagtg cgggcgggtt gggattcaac
     1081 acacctcgcg aatttcaggc cgatgacctg ctgatgttga aagtgatcct gccgccgttc
     1141 agcatgattg aaaccatggc caagatcatc cgggtgacgc cgctcgatga ggggaaggca
     1201 ggatttcatc tcgccaccca gtttgtcgat ctgggcgggg acgagcagga actgattatc
     1261 cggcatatcc tccagattca agccgagcgg ctacgaacga ggaaggccgc gcataatcat
     1321 ctctgaatgg tgagcaggat ttgaggcgct aaccgtctag gccgcccgtt ccggagaggg
     1381 agcggatggc tgcggcatcg tcgctggttt ggtgagctga gccaagagcg catcagccgc
     1441 tccgttgagg gcgacggaag ccggcgcggt cggttcgaac tccaccaccg gcaaatagct
     1501 gcgaaccgcc cggaccatcg ccggatcgtc cggaatctcg cccagcatcg tcaggtctcc
     1561 cccgacatac tccttcagca gctttttcaa ttgcagcacg ttgacccgtg atttgcctga
     1621 cacgcgattg acgatgaggt agggttgaaa cccttgtaag gtgttactcg ccgtctcttt
     1681 cgcattcgga tctgtctcgc ccacggcctg aatcacctcg tcgatactgg cgaaatcgcg
     1741 atcagacagc gcttcgctca ccgcgtcacg ggacaagaat gccgataata cacgtcgaat
     1801 ggcggccagc ttaatgaacc ggtagagatc aaggaccgag gtcggatctg gtgtcgccac
     1861 cgtgagatag cagtcggcca tgagaaaaaa atcgagcgca tgataactgg tgccggcgcc
     1921 gatatcgacg atgatgacat cagcctgtaa ctgggaaaag tggcgaatca ggcgtttttt
     1981 cttggcatag gggaggttgg cggtggctaa cgtatccccg gtaccgggca ggagttgcag
     2041 gaaaggatgg gccgaaatag gctgaagcac ctcctccaaa cgctccaccc gccgttcaac
     2101 aaaatcggtc agggtgcggg gagggttcag caacccgaac agaatgtgcg cgtcggcacc
     2161 gccgacatcg agatccgcca gcacaacgcg cttgccccgt ttagccaata gcatggccaa
     2221 attggcggca atgacgctct ttcctacacc gcccttccct gatcctatcg atacgatggt
     2281 ggccatccgc aactctctct cagctgtgtg tgtgttcgcc gatgtctgtt gtcggtcctt
     2341 cggcctagga acctgagcgg aataggccca tagggggaga acatctccct gcgactccag
     2401 cctgtgcctt attaaacccc atcgtccaag aacgggcaac taaagtttga gatggcgccc
     2461 accgatacca tatccgttat gaacaacgct attgcgccca caaccaacgt caacttcgac
     2521 agtgtatcgc cttcggaagg cgagattctc accgtgtcgg acgtcgcgcg gtttctgcgt
     2581 gtacccaaat ccacggtgta taaactcgcc cgtctcggag aattgccggc ttccaaaatc
     2641 ggaaagcact ggcggttcct ccgacgcgac attcacgagt ggatgcacag ccggacccag
     2701 cagcactgaa ctctcgttgg agagcagctg cagagacgac aacacgtgag cagcgcggac
     2761 tcccgggatg tccgttgatt gagctcacct ttagcggatc acacgaggta agggatggcc
     2821 gagtcaaacg cggtgcgcaa aacggaaatg cccgccccga agctaagccc ggacacattc
     2881 aagcagctcc gggatctcat ctatgagatg accggcattc actttcaaga caacaagacg
     2941 tatctgcttg aaagtcgctt gcttcctcgg ctgaaggcct gccgctgtca gacctttgaa
     3001 agctatctca actttttgcg cttcgatgcc taccgggacc gtgaaatcac cgagctctat
     3061 acggtcatca ccacgaatga aacatacttt ttccgcgatg aagcccagct cgatagtttc
     3121 atgaaggtga tgattccgga agtcatgaaa acgaacgccc agacgaaaca attgcgcatc
     3181 tggagcgccg cctgttcgac cggggacgaa ccgtatacct tggcgctgct gctgcgtgac
     3241 tatcctccgc tgaacggctg gaccatcgat atcctcgcaa ccgacatcag tgaaaatgtt
     3301 ctgaacatcg cacgaaccgc cacctattcc tcccattcgc tgcgcaaggt gccgccggcg
     3361 atgcttgcgc gctacttcac cgggaagaaa gaagaacaga cgctggtgca accggtgaaa
     3421 gacatggtgc ggttcatgaa cgtcaatttg tacgaccgac ccagactgaa gctgatccgc
     3481 ggcatcgata tcattttttg ccgcaactgc ctcatctatt tcgacgaaaa agccaaggcg
     3541 cagattgtct cggatctgcg cgacgccctt cgtcccaagg gatacttgat gatcggattt
     3601 tccgagaccc tgcacgacgc ggcaggccac ttcaaaacca ttcatgcggg ccgttcagtc
     3661 attcatgaga aacagtaagg aggacccatg ccggctgatc cgaatatgaa gattctcgtt
     3721 gtcgatgaca tgtccaccat gcggcggatc gtcaagaaca tcctgaaaca acttggtttc
     3781 aacaacctgg aagaggcgga aaacggtcaa gaagcgttga ccaaactcaa agccgatacg
     3841 tacggcttcg tggtgtctga ctggaacatg cccgtcatga tgggcatcga catgctgcgc
     3901 gccattcgcg cggatgaatc gctcaagaaa attccggtcc tgatggtcac ggcggaagcc
     3961 cagaaggaaa atctcatgga ggccgtgcag gccggcgtga gtaactacgt cgtcaagccg
     4021 ttcaccgcag agacgatgca ggacaaaatc aataagattt tcaagtaatc ggctcttaac
     4081 cggtgggggg gacgatggca caaccacgcg cggcggccca gtcgcaacca gcgacgcatg
     4141 aatcagagca ggaagaaaaa gcacctgata ccaagctcta tgaggaactc ggagaattgg
     4201 cccgcttcat cgacaccacg atgaagacct tgtccgaatt cagcacgccg gtcagcacct
     4261 cgaccgagca gctgccgcaa gcgctcagcc acctcaccaa tctcaagacc atgaccgagc
     4321 agggaactca tcgggtcatg ctcgaggttg aggccattca agacaatcac atgaaaatca
     4381 gcggcatgat caaggacctg acgctcgcca tgcagaaggc gcaggcggct cccgcactcc
     4441 tccagcagat gcaagccgtc ggcaatgcca tcaacgtgga cgacaagcgg ctgctcgata
     4501 tcatgacggc cttgtcattc caggatctgg tggcacaaag cgtcaacaag ctcgtcacca
     4561 tcctgcaaga agtcgaacac aaactgctcc aactcgtggt ggtcttcggc ccctatcaaa
     4621 agcaggcggc aaaaaaagag caggacaagg ccagcgaaat gctgaagcaa ttggaagcga
     4681 ccaagaatac atcgatgaat caggatttgg ctgacgaaat cctgaagcaa ttcggtttta
     4741 actgaggtgg gttatgagcg atgaaatgca ggaaatcctg aatgactttc tgactgagtc
     4801 caacgagatg ctggaagtgt tggaccagcg cttcgtgacg ttggaatccg atcccaacaa
     4861 caccgatctc ctgaacgaga tcttccgcgc gatgcatagc atgaaggggt ccgccggctt
     4921 cctcggcttc aaccatctgg tggatgtggc ccatcgcggc gagaacattc tcaacaagct
     4981 gcgccagggc gaaatggccg tgaacccggc catcatcagt atcattctcg aaaccatcga
     5041 cgtcatcaag gcgatcatgg ccgacattcg ggaatcaggc actgacaacc acgtcgccac
     5101 ggcatcgatt gccgcaaagc ttgacgacat tctgaacggc aacacagccc cggctgctgc
     5161 gccggtacag acggaagccg ttgcgccccc gaaagtcgaa gccgcctcgt cggcacctgc
     5221 cgcggccgct ccggtcgctg ctccccagac gctgggtgaa attctggtga acgacggcct
     5281 ggcctccaaa gagcaggtcc ttgatgccct tacggcgcaa cagcatcaac cggaaccgaa
     5341 gactccgctg ggcgaaattc tcttgcaggc aaaagccatt accgaacgcg ccctcgatca
     5401 agccctgcac aaacaagata aacagccgaa gccggtggaa gaagatgcca ccattcgcgt
     5461 ggaaacgaaa cgcctcgaca gtgtgatgaa tctggtcggc gagctggtcc ttgggcgcaa
     5521 ccggttgatc aagatcggca cccaattgga gcagcagcac gaatctgatc cgcaggttcg
     5581 tgtgctgagc gaaacactcg cgcaactcaa cctggtgacg accgacctgc agttagccgt
     5641 catgaagacc cgcatgctcc cgatcaaaaa agtgttcgcc aaactgcctc gcatggtgcg
     5701 cgacctttcg caaaaactga acaagcaggt tcatttggaa atgcgcggag aggaaacgga
     5761 gcttgataag tccgtcgccg atgagatcgg cgatccgctc gtacacctgg tgcgcaatgc
     5821 catcgatcac ggaatcgaaa ccccggccga gcgccaggcg aaaggcaaag caggggaagg
     5881 acaactgacc attgcggcga gccaggaagg caacagcatc gtcattcgga tcaacgacga
     5941 cgggcgcggt attcaggtcg agaaggtcaa ggagaaggcc ttggccaaag ggctgatcag
     6001 cgaagccgaa ctcgcgacga tggagcaccg tgaaattctc aatctgattt tcctgccggg
     6061 attcagcact gccgaaaaag tgacggatgt ctccggacgc ggcgtcggca tggacgtggt
     6121 tcgcacgaat atccgcaaaa tcaacggcag cgtggatctg gaatccgagc cggggaaggg
     6181 cagccagatc attatcaagc tgccactgac gatcgcgatt attcaggcct tgatggtgga
     6241 agtcgagcgt tcgattttcg ccattccgct gagcacggtc attgaagccg tgcgaatttc
     6301 tcgctctgag atcaagacca tcaacgggcg tgaagtcctg cacctgcgtg accgggtgct
     6361 gccgctcatt cgcctggctc aggagtttga cattccgacg gacgccaatc gtgagcgctt
     6421 ctacgtggtc gttgccgcat tgggagaccg tcgagtggga gtggtggttg atgagttgcg
     6481 atcccaggaa gaagtggtga tcaagtcgat ttgggattat ctcgaaaccg tcaagggcgt
     6541 atcgggcgcc acgatcaccg gcgaaggcaa ggtggtgctc attctggata cgtccgagct
     6601 ggttcaaaat gcacaagcgt ggcataaccc gggcatcgcc gcctgacaca agggtgtatg
     6661 ccctgagctg tgggcgggga gacgggttca agaatagtcg atgaactgag acgcgcgaga
     6721 tcggaggaag tatgtccaca ctgatcaatg aaatcgatgc caggaccagg ctagccggcg
     6781 caaaccagat ggaattgctg ctgttcaagc tcgggacaaa cgagattttc ggcatcaatg
     6841 tcttcaaggt gcgtgaggtg atgaagctgc cggagctgac ccagattccg gaagcggaca
     6901 gccgcatcgt cggcatggcg aatatccggg gcatcatggt ccccgtcgtc ggcctgaagc
     6961 gaagcttggg actgggaatg gaaaccgagg gacagggcaa cggggctccc ggcgggaatc
     7021 cctacctgat cgtctctgaa tataacggca gcttgcaggg ctttctcgtc gccggcgtgg
     7081 atcgcatcat tcgcttttcc tggtcggcca tcaagacgcc gccggccatc gtccgggaga
     7141 acaacaaggg agccgtcact gccgtcacga tgctggaaga cgggcggatg gtcttgatcc
     7201 tcgacgtgga aaaagtcttg aacgacattt gcccgcgcac ggacgacgat gtcttcgccg
     7261 ggatgacgat cgcccccgaa ctccgatcaa agtgcgtctt gtttgccgac gattcttcag
     7321 tggcccgcat gcagattcgc aaagcactgg atcgcctctc catgtcgtat gtgattgcga
     7381 cgaccggcgg cgaagcctgg caaaagctgc aagcgttggc ggatcaagcc accgcagaag
     7441 gtaaagagca agtggatcag atccagtgca ttctcagtga catcgaaatg cccgagatgg
     7501 acggctttac cttgaccaaa cacatccggg ctgatccgag actggcgcat ctgccggtca
     7561 tgctgcactc ctctctgacc ggtgcctgca acatggagaa gggacggacg gtcggcgcga
     7621 cggattacat cacgaagttt gacgcaaaaa tgctgggcga gaaactggcg ctctacattc
     7681 aaaacccgaa cgccaatgtg aaaaaagcgg cctgatatcg ccgcagaccc tggcgtcagg
     7741 gtgacggctg attcaagctt ttgtcagaga cggccgatgt atcgctaaag atcctatggc
     7801 acttccactc acacagccgc agagcgccgc gaagccggtt cgcgtcttag tcgtcgatga
     7861 ttccgcattc atgcggaaaa gtctgaccac gatgctcgaa gaaggcaaac agatccaggt
     7921 ggtgggagtc gcacgcaacg gtgaagaagc ggttcagcag gtacaacagc tcaagcccga
     7981 cgtcgtcacg atggatgtcg aaatgccggg gatgaccggg cttcaggcac ttcagcagat
     8041 catgaccaag aatccagtcc cggtcatcat ggtgagttcg gtgacggtcg aaggcgcgca
     8101 agagacattg caggcgttgg agtggggggc ggttgatttt atccccaaac aactcgatgg
     8161 cgtggcatcg aaaatcgccg agattcaaaa aatcctcgtc cccaaggtgc tcgcagccag
     8221 atatgcgggc agcaaactga aacgactgaa cgttacaggc gcaccgaagc tcgctgtgcc
     8281 agccgccaaa gcgttgagca gtcgctccgt ctccgtaaca cgcggcacca aactcattgc
     8341 gatcggatgt tccacgggtg ggccacaagc cttgtttgaa atcgtcccga cgatccccgc
     8401 cgattgtccg gccggcatcg tcatcgtgca gcacatgccg agttcgttta ccaaaccgtt
     8461 cgctgaacgc ttgaacaatt tgtgcgcatt ggaagtacgg gaagcggttg acggcgatga
     8521 ggtgaaagcg gggcgggtgt tggtggctcc gggcgggatg caattccggg tcgtgaagaa
     8581 gtccatcacg acgtcagtcg tcaaattgtc gccgaattat gaaaggcatg cccattcgcc
     8641 atccgtcgac atcatgctcc agtcggtcgc cgcattgttc ggagagcgca gcatcggagt
     8701 gattttgacc ggaatggggc atgacggact ggaggggatg aaggccatca aagcagcagg
     8761 aggtcgaacc gtggcgcagg acgaagcgtc aagtgtcgta tacggcatgc cgaaagccgt
     8821 ggtcgaagca ggatgtgcag agaaggtggt gtcactttca aaagtcatcg gcgagatcat
     8881 gaacatggtg tgacagggat cgttcattca accgttcacg catggacagc taggaggagt
     8941 tatggggagc atgatgaagc acatgtcgat tgggccgaag tttattatat cgatttccgc
     9001 agtagcccta gtggtgatc
//
LOCUS       DAOQEW010000151         9014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2558_length_9014_cov_7.586338, whole genome shotgun sequence.
ACCESSION   DAOQEW010000151 DAOQEW010000000
VERSION     DAOQEW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_9014_cov_7.586338"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..89
                     /locus_tag="PLS35_14525"
     tRNA            1..89
                     /locus_tag="PLS35_14525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Leu,seq:taa)
     gene            complement(361..705)
                     /locus_tag="PLS35_14530"
     CDS             complement(361..705)
                     /locus_tag="PLS35_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19935.1"
                     /translation="MTDDYARRKVQVIKEFLESQFPTPYVVEAIVPRRDPLADTKYKV
                     ILNNVVERTLLVCPAVVMDSFPTPDHLKESLVARNIIDKVKASTDSRICHDDLGTDDQ
                     NYQKPIRSVRGL"
     gene            1154..1495
                     /locus_tag="PLS35_14535"
     CDS             1154..1495
                     /locus_tag="PLS35_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19936.1"
                     /translation="MKVCAGEYDSRSGLESLVCTTCKHRGLRSREGIIPLFRGGHEFK
                     FSYGPSTRTVTVVLSSAAVNLWGTHGVNEEQLAKLAAEWTLLCGNTKKPVQLGIPSEE
                     FADFYLYFCRK"
     gene            complement(1594..2160)
                     /locus_tag="PLS35_14540"
     CDS             complement(1594..2160)
                     /locus_tag="PLS35_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19937.1"
                     /translation="MSKPIPLECPRCLLANPDAAQYCECGYDFTPHFEGFPLKSSPGA
                     APRKRLFSEEMGRGSFVLGILLIWLALSVGELYRIRIELPRRPKMELGFSSLMVGMTD
                     LAAALVVGAIGWACVWIVLAAQSKRDGSLCPKRTTFVVTLIAAGLLFVTRTIDPSVLV
                     QHVMQVLFVLAIAIASYYAGCRNWLGKW"
     gene            2231..2623
                     /locus_tag="PLS35_14545"
     CDS             2231..2623
                     /locus_tag="PLS35_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNI19938.1"
                     /translation="MQASTPSFRFACFGALFAAAAVAAGAFGAHALKSILDPAMLTVY
                     ETAARYQMYHALGLFVVAWLSRETHSPLAIKAGWLFCAGILLFSGSLYLVALAGIKWM
                     GALTPLGGVSFISGWACVAWTAWRAGRP"
     gene            complement(2620..3588)
                     /locus_tag="PLS35_14550"
     CDS             complement(2620..3588)
                     /locus_tag="PLS35_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19939.1"
                     /translation="MPNPRGCLFVVACCVVTLLTSSIAVRAEEASPPPKPTLANRLPA
                     FQDVAKANAAQLSALPAQGDALSLFKTIIGPQLGLGDAAMTIGAKGLTPTMSQELLIS
                     DLTRAANELVRGLAAWQLVQATRDLDTSGTAESRQEMHKRIEAQTAWLSDGTTLAPAL
                     ERVQTLTAAAPTEETAPLLGGAVAQIEAWALDTVHREWFRIYNWKDQVRQQRGLVRLC
                     GTWQWSIHNHQNHREEKTAVIFAPPGSVSGTSPAEIIVLGDSVYLRWETRAGVQEDSL
                     LFSGEGQRLEGTFVNSAGGWGSITGKRTATCFNDGGKPTSPRRQHH"
     gene            complement(3688..4479)
                     /locus_tag="PLS35_14555"
     CDS             complement(3688..4479)
                     /locus_tag="PLS35_14555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNI19940.1"
                     /translation="MTTQTAPFSLDQIGTGTARFPSGMAIPTATDAMVDPYIRTRVSK
                     DVHVECIQFWPQDKVTYPGIVLLHDWWGMNSQITALGARLACEGYGVIIPKLYGRLGG
                     MVTANAEVAEALAAKCNEQLLLQDINTCCEYLNTTERTKRNIHGVVGFGLGATLAIRF
                     ACQRKRLRAAVAYYGKVTAPDQLTNMMSPLLYHQAEHDTWATQADADLLRSAAAQGKR
                     IEIKTYPGTTHAFCDETRPAEHNAEATAQAWDATVAFLKTCFQGT"
     gene            4651..5619
                     /locus_tag="PLS35_14560"
     CDS             4651..5619
                     /locus_tag="PLS35_14560"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNI19941.1"
                     /translation="MPLGIGLIGLGHHGSRYAKHLLHDLPEARLVAVCRRRAERGNGL
                     PDAATVPCYSDYRELIADSRVEAVVVVTPPTLNRAMCTSVAQAQKALLVEKPLATTAE
                     DARAIMQAARASGQVMMTAQTLRFDAAVSALRARQAEAGALQYLSLASRMEPHADSED
                     WGFGGRGCVLEIGIHLLDLVRVLTQDEVLTVCCEMDRVAPDGAERRMLGRLTTRSGLV
                     CVLDVSRVTSGRIGRVELIGSEGQLSADWCGQRVTRVSARHGAGEWPTAAEPTVLNTL
                     RAFVRAVRDGLPPPITIEDGKRAVEIAEACYLSASQGGRAVTVEYR"
     gene            complement(5621..5857)
                     /locus_tag="PLS35_14565"
     CDS             complement(5621..5857)
                     /locus_tag="PLS35_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNI19942.1"
                     /translation="MATKAYILIKVKAGKGKSVLTALKGIAGVEQIHACFGQPDIFVF
                     INVADERALSDVVITRIHAIEGVEETDTHIVAET"
     gene            complement(5928..6566)
                     /locus_tag="PLS35_14570"
     CDS             complement(5928..6566)
                     /locus_tag="PLS35_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19943.1"
                     /translation="MPATPAARPGEVPDFFMRVSLAITLLSLAPLAPDIGWSQQATGE
                     TTPVMRTVAQPSRTASVYIGSVMAMLATFEDAGILPPEGTPQANRIIKAVIQFQSAFL
                     KSDHPAIRQFFTEAHRAKLGPRADEVETSFRQSGWSADTFDAVISAGQLETAWNTDGL
                     SDGFREFNIGKQDFDILAELYQQSSRAFSTQGKSFQEVYAQRRREMPGAKSE"
     gene            6792..8321
                     /locus_tag="PLS35_14575"
     CDS             6792..8321
                     /locus_tag="PLS35_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNI19944.1"
                     /translation="MLAKVLSAAIVGIDAHLVDVEVDISAGLPQFSIVGLPDATVRES
                     RDRVRAALKNSGFQFPIKKVTVNLAPANIKKEGAGLDLAIALGILVAEDVIAAEAVNN
                     TVFVGELSLDGRLKPIPGALSIGVVCRHRHRIFVTAENAEEAALADGAEVLPIHTLPQ
                     AVECLRGVQPIAALLLTGDGGAASLLQEDEDFADVKGQAHAKRALEVAAAGGHNLLMM
                     GPPGSGKTMLARRLPGILPVLAQEEALETSRIHSVVGQLSRDQPLMRRRPFRAPHHSI
                     SEAGLIGGGTMPRPGEVSLAHNGVLFLDEAGEFGRATLDALRQPLEDGHVTVTRASGS
                     LKFPARFMLVAAMNPCPCGYYGDRTRDCVCSVAQVRRYRGRLSGPLLDRLDLQIEVPA
                     VPIRALGDDAPVTDSSATIRTRVMAARARQAERYRRDGIYTNAQLKPRHLKQYCALDS
                     QGRELLEQAMARLGFSARAHGRILRVARTIADLAESDSIGPAHLAEAIQYRSFDRRVE
                     L"
     gene            8318..8548
                     /locus_tag="PLS35_14580"
     CDS             8318..8548
                     /locus_tag="PLS35_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19945.1"
                     /translation="MRETPVIHTARVFAWWFIVGATMALAVILLQGGIREAMQAQGSV
                     WELKLVEFLTTILGGGLLGGCIALILDRIKKS"
     gene            8591..>9014
                     /locus_tag="PLS35_14585"
     CDS             8591..>9014
                     /locus_tag="PLS35_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19946.1"
                     /translation="MDIEVGSNLYRNSNGIIDIEGVPQFEVAIKEPARALLVNFALFD
                     EVGRMMAKVVDSNLTFNERRAYQLAKSPTSVSLKHEESGTVVFALELREGNRVVFSKG
                     SFHTIKGHRLDVSQTEWRIDKKHFSGKETDTKGGAVFLG"
BASE COUNT         1760 a         2515 c         2809 g         1930 t
ORIGIN      
        1 gcgggagtgg tggaatcggc agacacccag gacttaaaat cctgtgtcat accaatggcg
       61 tgcgggttca agtcccgcct cccgcaccaa aagaccggtc agcggtgttg tctctcctcg
      121 actcgtcctc tctgaactct gctctccagc ctgattgacc agttctcaca ctacacgccc
      181 ttcacttttt cgagcgcatc agtagattct cggtgattcg gctgatcgga agatatgcgc
      241 gggacgaacg atggatcgtg atgcaggggg tgtcactgcg acgcgagacg acgttccacc
      301 atcacagccg tgtaatgctg gacgggtctg tcgataaacg ggaagcggaa cagcggtgcg
      361 ctagaggccg cgcaccgatc gaataggttt ttgataattt tggtcgtcgg tacctagatc
      421 gtcgtggcag atccgggagt ctgtcgacgc cttgaccttg tcaatgatgt tgcgggccac
      481 gagtgattcc ttcagatggt ccggcgtggg gaagctgtcc atcaccacgg caggacagac
      541 cagaagggtg cgctccacca cattgttgag gatcactttg tatttcgtat cggcgagtgg
      601 atctcttctc ggcacaatgg cttccacgac atagggcgtt ggaaattgcg actcgagaaa
      661 ctccttgatg acttgtacct tcctgcgcgc gtaatcgtct gtcacggtcg atggtccttc
      721 cgaattccgc ccctgtcggt ttgccgacgg caggggaagc agcggttgtg gtttcagatc
      781 tcttgtggca cagtgccgtc ccgtagagtg ctgatattaa gagaaaaacc gactgaaaac
      841 cagaggctgg tcccggccgg tcgcaaatcg ctgcgatctt ctcacgactt cacagcgaat
      901 gaccgggcgg agccagggtc tgttgtggcg tctgatatac ttgccatagt taaccatggc
      961 atcgtgtcca tgctgagctc cggtgggaat tttgtagatg gttcaccgca tcgctgaggt
     1021 cactgtgcag gaaaacgacg gttctggagc ggcaggcgat ccggcggcac catccacacc
     1081 agtttcatcc ctcgcacaga tctcgaaaga ctcagatggg gcccgttgcc cggttcagaa
     1141 gtgtgacggc cgcatgaagg tgtgcgccgg cgagtacgat agccgcagcg ggttggaatc
     1201 tctggtctgc acgacatgta agcaccgggg attgcgttcg cgcgagggca tcatcccatt
     1261 gtttcgggga gggcacgaat tcaaattcag ttacgggcct tccacgcgca cggtcaccgt
     1321 ggtattgagt tccgcagcgg tgaatttgtg gggcacacac ggggtgaatg aggaacaatt
     1381 ggcgaagctt gcggcagaat ggacgcttct atgcggtaat acgaagaaac cggtgcaatt
     1441 aggaattccg tctgaagaat tcgccgactt ctatctctac ttctgcagaa agtaacgcca
     1501 gtttagcatc cagcgtagtc acctccaccg cagctcattc tcctacagtt ttttcgaata
     1561 gcctgaggcg ggtgctgcac gtcggttcat ctatcaccat ttccccagcc aatttctgca
     1621 tcccgcataa tacgacgcaa tcgcaatagc caggacgaag aggacctgca tgacgtgttg
     1681 gaccaacacg ctggggtcaa tggtccgtgt cacaaaaagc aggccggcgg cgatgagggt
     1741 cacgacgaag gtggttcgct tggggcagag gctgccgtcg cgtttgcttt gcgcggcaag
     1801 gacgatccag acgcaggccc agccgatcgc ccccacgacg agagccgcgg ccagatccgt
     1861 catgccgacc atcaaactgc tgaagcccaa ttccattttc gggcggcgag gcaactcgat
     1921 acggatgcgg tagagttcgc cgaccgacag cgccagccag attagcagaa tgccgagcac
     1981 aaaactcccg cggcccattt cttccgagaa caagcgtttc cgcggcgcag ctcccgggga
     2041 ggatttcagg ggaaagcctt caaagtgcgg ggtaaagtcg tacccgcatt cgcaatactg
     2101 cgccgcatcg gggtttgcca ggaggcagcg gggacattca agcggaatgg gtttgctcat
     2161 ttgggatcgg cttcatggta aaatcgcgcg ccctgcctcc ggccaatgat ggaggcttca
     2221 cgaggtctgt atgcaggcat caactccatc gttccgattc gcatgtttcg gggccctgtt
     2281 tgcggcggcg gccgtagcag ccggcgcgtt cggcgcccac gcgctgaaat cgattttgga
     2341 tccggccatg ctgactgtat atgaaacggc ggcgcgctac caaatgtacc atgctctcgg
     2401 cctctttgtc gtagcctggc tgagtcgcga gacgcatagt ccactggcga tcaaggctgg
     2461 ttggttgttc tgcgccggga tcctcctgtt cagcggaagt ttgtatcttg tggcgctggc
     2521 aggaatcaaa tggatgggcg cactgacgcc cttgggcggg gtgtcgttta tcagcggatg
     2581 ggcttgtgtc gcctggaccg cctggcgagc gggccgccct tagtgatgtt gacgacgcgg
     2641 tgacgtcggt ttgccgccat cgttgaagca ggtggcagtg cgtttgccgg tgattgatcc
     2701 ccatccccca gccgaattga cgaaggtgcc ttccaaccgt tgtccctctc ccgaaaagag
     2761 cagactgtct tcctgcacac cggccctggt ttcccatcgc agatagacgc tatcgccgag
     2821 gacgatgatt tccgctggac tggtgcccga cacggatccc ggtggcgcga agatgaccgc
     2881 cgtcttttct tcgcgatgat tttgatgatt gtgaatggac cattgccacg tcccgcacaa
     2941 ccggacgagg ccgcgctgtt gtctcacctg gtctttccaa ttgtagatcc tgaaccattc
     3001 gcggtgcacg gtgtcgaggg cccacgcttc gatctgggca acggctccgc cgagcagggg
     3061 ggccgtttct tccgtcggcg cggctgctgt tagcgtctga acacgctcca gcgctggagc
     3121 cagcgttgtt ccgtcgctca accaggccgt ctgggcctcg attcgcttgt gcatctcctg
     3181 acgcgattct gctgtgccgg atgtatcgag gtcgcgggtc gcttggacca attgccaggc
     3241 agcgagtccc cggaccagct cattggcggc tcgcgtcaga tcactgatga gcagttcttg
     3301 gctcatggtc ggggtcaatc ctttggcccc gatagtcatc gcggcatcac cgagtcccaa
     3361 ttgcggacca atgatcgtct tgaagaggga aagcgcatct ccctgcgcgg gaagggccga
     3421 caactgcgcc gcattggcct tcgccacatc ctggaaggcc ggcaggcggt tggccagagt
     3481 cggcttgggt ggaggagatg cctcttcggc acggaccgcg atcgatgaag tcaacagggt
     3541 gacgacgcag caggcgacga cgaagaggca accacgagga ttcggcatgc ggctatgttc
     3601 tttctatgcg agaaggagga gtaccggcat ggggcgacca gagcacatcg gccagcgccg
     3661 tcaccacctg ggtcaccctc gatacggtta ggttccctgg aaacaggttt tgagaaatgc
     3721 cacggtggcg tcccaggcct gcgcagtggc ttcagcattg tgctccgctg gtctggtttc
     3781 gtcgcagaag gcgtgcgtgg tgcccggata ggtcttgatc tcgatgcgtt tcccctgggc
     3841 cgcagcagac cggagcagat cggcgtcggc ttgtgtcgcc cacgtatcgt gttccgcctg
     3901 atgatacagc aggggactca tcatgttcgt gagttgatcc ggcgccgtga ccttgccgta
     3961 ataggccacc gcggcccgca accgtttgcg ctggcaggca aaacggatgg ccaacgtggc
     4021 gcccaggccg aatccgacca cgccgtggat gttccgcttc gtgcgttcgg tggtattgag
     4081 atattcgcag caggtgttga tgtcctggag caggagttgt tcgttgcact tggccgccaa
     4141 cgcttccgcg acttcggcat tggccgtcac catgccgccg agccggccat agagtttcgg
     4201 gatgatcaca ccgtatccct cacaagccaa ccgtgcgcca agggccgtaa tctgagagtt
     4261 catgccccac cagtcatgca ggagcacaat cccgggatac gtgaccttgt cctgcggcca
     4321 gaactgaatg cattccacat ggacgtcttt gctgacgcgc gtgcggatgt agggatccac
     4381 catcgcatcg gtcgcagtgg gaatcgccat tccactcgga aagcgagccg tgccggttcc
     4441 aatctgatcc agggaaaatg gtgcggtctg cgtagtcatg ggtgatcggg tcctttctcg
     4501 attggcgtct gcgtccagcg ggtagccgcc tggtactata gggagcggat ttgcacattg
     4561 tcaatcgacg gaatggtagc gggtgaggag tcttgacccg tcagagtgcg gccggtacag
     4621 tgcgcgccaa cgatgcctca cgaatctcgc atgccgctcg gaatcggctt gatcgggctt
     4681 ggccatcatg gcagccgtta cgccaaacat ctcttgcacg accttcccga ggcccgtcta
     4741 gtcgcggtgt gccggcgtcg ggccgagagg ggaaacgggt tgcccgatgc ggcgaccgtg
     4801 ccctgctatt ctgactatcg cgaactgatt gccgattcac gggtcgaagc ggtggtagtg
     4861 gtgacgccgc ctacactcaa tcgggcgatg tgtacgtctg ttgcgcaggc gcagaaggcg
     4921 ctattagtgg agaagccgct ggcgacgacg gcagaagatg cgcgtgccat catgcaggcg
     4981 gctcgggcga gcgggcaggt aatgatgacg gcacagactc tgcggttcga tgccgcggtg
     5041 agcgcgctgc gtgcgcggca ggcggaggcc ggtgcgctgc agtatctcag cctggccagc
     5101 cggatggaac cgcatgcgga cagtgaagac tggggattcg gcgggcgagg ctgtgtcctg
     5161 gagatcggaa tccatctgct ggacctggtg cgtgtgttga cgcaagacga ggttctcacg
     5221 gtgtgctgtg agatggatcg tgtggcgcct gacggtgccg agcggcggat gctggggcgt
     5281 ttgacgacca gaagcggatt ggtctgcgtg ctggatgtgt cgcgggtcac atcggggcgg
     5341 atcgggcggg tcgaactgat agggagtgaa gggcagttat cggcggactg gtgcgggcaa
     5401 cgtgtaacac gggtctccgc gcggcacgga gcaggagaat ggccgaccgc ggcggaacca
     5461 accgtactca acacactacg cgcttttgtg cgagctgtcc gcgacggctt gccgcccccc
     5521 atcaccattg aagacggcaa gcgcgccgtt gaaattgcag aggcctgtta cctctcagcc
     5581 agccaaggtg gccgggccgt cacggtcgaa taccggtgac ctacgtctcc gcgacgatgt
     5641 gcgtatcggt ttcttccacg ccttcgatgg catgaatccg cgtaatcacc acgtcggaca
     5701 gcgcccgctc atcggccaca ttgataaaga cgaaaatgtc aggttggccg aaacaggcat
     5761 ggatctgttc gactccggca atgcccttca gcgccgtgag aacagacttc cctttaccgg
     5821 ctttgacctt aataagaatg tacgctttcg ttgccatgat gcctcctcaa gctgcaattc
     5881 gtcacatagt gcggatgatg ttggctgcgc tcgttgctat gaaatagtta ttccgatttg
     5941 gccccgggca tttcccggcg acgctgggcg tagacttcct ggaagctttt gccttgagta
     6001 gaaaatgcgc gactgctctg ttggtacagt tccgcgagaa tatcgaaatc ctgttttcca
     6061 atattgaact cccggaaccc gtcactgaga ccatcggtgt tccaggccgt ttcaagttgt
     6121 ccggccgata tcaccgcgtc gaaggtatcc gccgaccaac cgctttggcg gaatgaggtt
     6181 tccacctcgt cggcccgtgg acccagcttg gcgcgatgag cttccgtaaa aaactgacgg
     6241 atggctggat gatcgctctt gagaaaggcg gattggaatt ggatcacggc tttgatgatc
     6301 cgattggcct gcggggtgcc ttcaggcggc agaatcccgg cgtcttcaaa cgtggccagc
     6361 atggccatca ccgaaccgat gtagaccgat gccgtccgtg acggttgggc aaccgttcgc
     6421 atcaccggtg tcgtttcacc cgtcgcctgc tgactccaac caatatcagg agcaagcggc
     6481 gcgagagaga ggagggtgat ggccagagag accctcatga agaaatccgg cacctctccc
     6541 ggccgtgctg ctggagttgc aggcatgaca ctgctcgtct tggcgcgatc acggcaatcg
     6601 aagagagacg gcatggcggc attatacaga agcgccgcta cgcgcgctac cgcgggaaac
     6661 caccgcctcc acgagctcgc cgcgacattg aattcagcgg aggcaactgc ctataattca
     6721 gccactggag ccactttgcg acctgttacc catcaatttg ttgaccgact gatgacgttc
     6781 cgggggaatc tgtgcttgcc aaggtcttga gtgcagcgat tgtgggcatc gacgcacatc
     6841 tggtggatgt ggaagtcgat atctccgccg gattgcctca gttctccatc gtcggtcttc
     6901 ccgatgcgac ggtacgtgag agtcgcgatc gggttcgtgc tgccctgaag aatagcggct
     6961 ttcaatttcc catcaaaaaa gtcacagtca atttggcgcc ggcgaatatt aagaaagaag
     7021 gcgccgggct tgatctggcc atcgccttgg gcattttggt ggccgaagac gtcatcgctg
     7081 cggaggccgt caacaacacg gtgttcgtcg gggaactctc gctggatggg cgtctcaaac
     7141 cgattccagg cgccctctcg atcggcgtcg tctgccgaca tcgtcatcga atttttgtca
     7201 ccgccgaaaa tgctgaggaa gcggccctgg ctgacggcgc agaagtactt cctattcaca
     7261 cgctgccaca agccgtggaa tgtttgcgtg gcgtgcaacc catcgccgcg ttgttattga
     7321 cgggcgatgg aggagccgca tcccttcttc aggaggacga agactttgcc gatgtgaaag
     7381 ggcaggcgca tgccaaacgc gcattggaag tggcagccgc cggagggcac aatctcctga
     7441 tgatggggcc tcccggatcc gggaaaacca tgttggcgcg ccgccttccc ggaatcctgc
     7501 ccgtgttagc gcaggaagaa gctttggaga caagtcgcat ccacagtgtg gtgggacaac
     7561 tttcacgaga tcagccgttg atgcgtcgcc gtccgtttcg cgccccgcac cacagtattt
     7621 cagaagcggg gctcatcggc ggtggaacca tgccgcggcc cggcgaagtc tccctcgcgc
     7681 acaatggggt cttatttctg gacgaagccg gcgagtttgg tcgtgccacg ctggacgcgt
     7741 tgcgacagcc tctggaagac ggacatgtca ccgtcacgcg cgcgagcggc tccttaaaat
     7801 ttcctgcccg ctttatgctg gtggccgcga tgaatccctg tccctgcgga tattatggcg
     7861 atcggaccag ggattgcgtc tgcagtgtcg cgcaggtgcg gcggtatcgg ggacgattat
     7921 cgggaccgct cctggaccgc ctggatctgc agatcgaggt cccggctgtg ccgattcgtg
     7981 ccctcggcga tgacgcgccg gtgaccgatt cctccgcgac tatccgcacc agggtgatgg
     8041 cggcacgcgc tcgtcaggcc gaacgctacc gtcgcgacgg catttacacg aatgcgcaat
     8101 tgaaaccgcg ccatctgaag cagtattgtg ctctggatag ccaggggcgc gagctgcttg
     8161 agcaggccat ggcgcgatta gggttttcgg cgcgcgcgca tggacgtatt ctccgtgtcg
     8221 cccggactat tgctgacttg gccgagtctg atagtatcgg gccggcccat cttgccgagg
     8281 ctattcaata tcgaagtttc gaccgtcgag tggagttgtg agagaaaccc ccgtcattca
     8341 taccgcgcgt gtcttcgcct ggtggttcat cgtcggcgcg acgatggcct tggcggtcat
     8401 cctcctgcaa gggggcatca gggaggcgat gcaagctcaa gggtcggtct gggagctcaa
     8461 actcgttgaa tttctcacga ccattttggg tggtggctta ttgggcggat gtattgcgtt
     8521 gattttggac cgtatcaaga aatcgtgatg aggcatctcg gaatgccggg cagcataagg
     8581 agtggtggcc atggatattg aagtcgggtc gaatctgtat cgaaactcca acgggatcat
     8641 cgatatcgag ggcgtgccgc aattcgaagt cgcgatcaag gaaccggcgc gggcgctgct
     8701 cgtcaatttc gccctgttcg atgaagtggg acggatgatg gcgaaggtgg tggacagcaa
     8761 tctgaccttc aacgagcgcc gtgcctacca gctcgccaag agtccgacga gtgtctcgct
     8821 caaacacgaa gaatcaggga cggtcgtctt tgccttggaa ctgcgtgagg ggaatcgcgt
     8881 cgtattcagc aaggggtcgt ttcatacgat caagggccat cgtctcgacg tgtcccagac
     8941 ggaatggcgg atcgacaaga agcacttcag cggcaaggag acggacacca aaggaggggc
     9001 ggtcttttta ggat
//
LOCUS       DAOQEW010000152         8973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2576_length_8973_cov_9.291545, whole genome shotgun sequence.
ACCESSION   DAOQEW010000152 DAOQEW010000000
VERSION     DAOQEW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_8973_cov_9.291545"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1067
                     /locus_tag="PLS35_14590"
     CDS             30..1067
                     /locus_tag="PLS35_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI19947.1"
                     /translation="MKALMSVGALIGVLGLLMLIGMIFGVVPSNTVRLVEGYMPMQVL
                     SELAVFVAGFTGLSYLANSMGMAIPRFWQGVLFWTFVQTYLKFRIYPPIPFSVRAMYG
                     VVSFVAVFMWVSANEEDWKKFRQPILNVLDANTGFHKALRTLYLILLPILIGGFSFLT
                     MKPSVDEPIELRTVHPAPPASTKVHGKTYTLQTSQNPYRVNLEGKYDQAYSNKLIVEQ
                     GMGRLMAPNANPWDDKAEGYLKYVREGGEIFFQNCHFCHGDNLNGRGLHAFAFNPIPA
                     NFTDPGTIAQLQETFIFWRVAKGGIGLPNEGFPWASVMPPWEQHLTTDEIWKVILFEY
                     WHTGYYPRTWD"
     gene            1058..2899
                     /locus_tag="PLS35_14595"
     CDS             1058..2899
                     /locus_tag="PLS35_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI19948.1"
                     /translation="MGLILNLTNNLGEQVVRLTMSKLRMNVKASPIIGAALGALLLSA
                     SAVGIGQAADLPEGFAKGDLAPAPPADMIEAGKRVYFTKCVWCHGVDGAGDGPGADRL
                     WPRPRNFNQGTFKIRHTASGELPLFNYNEKTPDSGKNDLFDTVTHGLPGSAMPPWEGI
                     LTEEQRIQVLSFVTTQLVKDRKFTDKQSETQTILQLGTIKPAEATEDSIKKGAQLIVD
                     KKCIECHGVEGRGDGNAFNLKDDWGFSIQPANWHKCWNFRGSRQDPYNVRNIFRTFST
                     GVNGTPMPSFADSTSIEDRWNIANFVNSLCERDEEGKPLGIDPLTDKPKINFVIPSSP
                     VEGEISSDPENEMWKKQGRRYVAMGGQITHKPRNFVNRIDDIWVKSLYNDKSVVYLIQ
                     WDDRTKSIAEGKLPWAPTQVNVENFGVKEQAPKTGEEGSIAAAQNNYAVYNDGIAFQF
                     PIKWQEIPAPFKPRYLFGDAKFNADILKWEADGSLRSFKGTGWDQDFEERDDFEEKVK
                     LLKSEWKNGQWTVMISRPLKGSKDDYDEYTRFDIGKYIPMVFFAWDGHNGDAGRKMAV
                     SAFYYTILQPPIPQEVYIYPAVIAVGVVFLEGWMLTRRANKKKGKVL"
     gene            3000..3608
                     /locus_tag="PLS35_14600"
     CDS             3000..3608
                     /locus_tag="PLS35_14600"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNI19949.1"
                     /translation="MIDDISGVLVAGGRSRRMGQDKRFMKVGGASVFERTLSVLRVTF
                     AENIVVLAEPIEGLDVQRCPVVYDLIPNAGSLGGLYTGLMTASRSRVFALACDMPFLD
                     PEVIRFMLSQDESADVIVANLGGRFQPMHAVYSKRCVPFLQAMAQRNELKIQNLFHQN
                     ALRMKILTENHLQAFGPGFRSFQNINTPSELASAEASPSIHP"
     gene            3647..4624
                     /locus_tag="PLS35_14605"
     CDS             3647..4624
                     /locus_tag="PLS35_14605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNI19950.1"
                     /translation="MVIHPGALGDVLLARPVLRALRCQFPQHEIALIAANAVGSLLCH
                     SAELDRAFSLEENYLSQLFAGFERLSDSFRTWLGQCELVVGWLSDADGAVTNTLRLAG
                     VQQIQLQTPFSSALATTHQADRYWEAVGMEGQREDFTSSLMLPAELERQGQDVLQAYD
                     WDGSSPLILVHPGSGSHNKCMDAWRLAKVIEWLIEARMAPMLLEGPADCRAVAQVLSS
                     LTVPVPVIRELSLSVVAGVMSQAKLYLGHDSGMTHLAAALAVPTIACFGPTHASRWSP
                     QGRAVAVISGMPCVCSDWSKVETCHDKVCLHIPVESLIEACRSQLLRLS"
     gene            4723..6477
                     /gene="argS"
                     /locus_tag="PLS35_14610"
     CDS             4723..6477
                     /gene="argS"
                     /locus_tag="PLS35_14610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNI19951.1"
                     /translation="MAQGVVQDRVADALAGALRSAQQRGILKIEQMPTINLEAPKRPE
                     WGDVSCTVAMSLSASERRPPFEIAQIIADHIQDRDALFSRVDIARPGFLNLTLQPDLW
                     MEVLRHIETQRAEYGHSRLGQGRRVLVEYVSANPTGPLHVGHGRGAAVGQALVRLLRA
                     TGHEVVSEYYINDAGRQMKLLGVSVLARYLESCGQAVPFPEDGYQGEYIRAVAARVKA
                     EQGVTLLSLSSSEAEQRSKDFAYRELLALIRQDLETFGITFESWFSEASLLSSGAVEQ
                     VLSELRSRDLLFDQDGAQWFRSSAYGDEKDRVVRKQEGDYTYLASDIAYHRDKLRRGF
                     DLLVDVWGADHHGYIPRMQAVVQAYGYPKERLRVVLVQMVNLLRGGRKVEMSKRAGEF
                     ITLREVMDEVGADAAKFFFLMRDSSTHLDFDLELAKQQSQENPVYYVQYAHARISSLL
                     RVAASRGIECPLPSQADLKLLNDPDELAIIRKLSVFPVVLEASAVELEPHRMAYYLRE
                     LAGLVHPFYNKHRILPPMTDLDATPAAPTEPSASTSRVPEAVSPGLTGARLALMWSVQ
                     QVVRNGLTVLGVSSPEQM"
     gene            6490..7647
                     /gene="tgt"
                     /locus_tag="PLS35_14615"
     CDS             6490..7647
                     /gene="tgt"
                     /locus_tag="PLS35_14615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNI19952.1"
                     /translation="MLSFRITHEEAGGRARVGVLTTGRSEVATPAFMPVGSLGNVRSV
                     DGEELLKMGYGLILNNAYHLYLRPGHKVVEELGGVHGFTAWPGAILTDSGGFQVFSLA
                     KFCKVTDEGVVFQSHIDGSSRFISPETSIEIQEALGADIIMAFDHVVALPSSLDQVRA
                     AAKRTSLWARRCVEAKRRTDQSLFGIVQGGLDQSLRVESARDLVSVGFDGYAVGGLSV
                     GEDKADMYAMLDVTVPELPSAKPRYLMGVGMPEDLVEGVARGIDMFDCVVPSRHGRTG
                     WLFTSFGRVVIKQARYARDEQPIDPACRCPVCTRYTRAYLHHLFGVKEMLGARLNTIH
                     NLWFFAKLMEEIRNAVRSGTFQEFRREFHRTYVVDRPGGEGRSDVDNTNMS"
     gene            7668..7988
                     /gene="yajC"
                     /locus_tag="PLS35_14620"
     CDS             7668..7988
                     /gene="yajC"
                     /locus_tag="PLS35_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNI19953.1"
                     /translation="MWDSVAWAQGTSGNAGAGGGLLSLVPFVLIFVIFYFMLILPQQK
                     RQKQAKAMLETLKKGDKVVTASGIWGTITNLGKETVTLQIADTTKIKIQKEHIARLRG
                     EEED"
     gene            8024..>8973
                     /locus_tag="PLS35_14625"
     CDS             8024..>8973
                     /locus_tag="PLS35_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNI19954.1"
                     /translation="MKKVGGRLLALVTMVVASIIFFLPSYQPLYKELPRWVREVLPDK
                     GITLGLDLQGGIHLVLEVEEDRAVEIAVERTAAGLQDLLVEKKIPAESVKRTGSSQIT
                     IGFQNAELKAQVQKLLDEFPTYVENEGAGSGNKVVWELREAEIKRIKDSAINQALETI
                     RNRIDQFGVAEPLIQRQGLKQVVVQLPGIKDAKLAKDLIKQTALLEFKLLDDDNQLKL
                     DLPGRIQKGKEREEAFLKQVEAKVPEGDQILFERIVEKESGQEWLAPYLVKKRVMLAG
                     DVLSDARVSIGQFNEPYVSVTFDAKGAREFDRITGENVKKR"
BASE COUNT         1964 a         2213 c         2704 g         2092 t
ORIGIN      
        1 gctattgcgg aagaaaggga gatgaggaca tgaaagcatt aatgtcagtc ggggccctga
       61 tcggcgtcct tggactgctc atgttgatcg gcatgatctt cggagtagtt ccatcgaaca
      121 ccgtcaggct cgttgagggc tacatgccca tgcaggtatt gagtgagctg gcagtgttcg
      181 tagccggctt caccggcctg agctatctgg ccaattccat gggaatggcc attcctcggt
      241 tttggcaggg agttttgttc tggacatttg tccagacgta tctcaaattc cgtatttacc
      301 caccgatccc tttcagtgtc cgagccatgt acggcgttgt gtcgtttgtc gccgtgttta
      361 tgtgggtatc cgccaatgaa gaggattgga agaagtttcg tcagcccatt ctgaacgtgc
      421 tggatgccaa taccggattc cacaaggctc ttcgcacgtt gtatctgatt ctcttgccta
      481 tcttgatcgg agggttttca ttcttgacga tgaagcccag cgtggatgag ccgatcgaat
      541 tgcgtactgt gcatccggcg cctcccgcta gcacaaaagt gcatggaaag acctatactt
      601 tgcaaacctc gcagaaccct taccgtgtca atctcgaggg taagtacgac caggcctaca
      661 gcaataaact gatcgtcgag caaggaatgg gtcgtttgat ggctcccaac gccaacccat
      721 gggatgacaa ggctgaaggg tacttgaagt atgttcgaga aggaggagaa atcttctttc
      781 agaattgtca cttctgccac ggagacaatt tgaacggtcg tggacttcat gccttcgcgt
      841 tcaacccgat tcctgcgaat ttcaccgacc cgggaacgat tgcccaattg caagagacct
      901 ttattttctg gcgcgtcgct aagggcggaa ttggtttgcc gaacgaagga ttcccttggg
      961 cctccgtcat gccgccatgg gaacaacacc tgaccactga tgaaatttgg aaggtgatct
     1021 tgttcgaata ctggcacacg ggatactatc ctcggacatg ggactaattc tgaatcttac
     1081 aaacaacctg ggagaacagg tggtgaggtt aacaatgagc aaacttcgta tgaacgtaaa
     1141 agcatctccg atcatcgggg ctgctcttgg tgcccttctc ctatccgcaa gcgcggttgg
     1201 gattggccaa gcggcagatc ttcctgaggg tttcgcaaag ggggatttgg ccccggcccc
     1261 gcccgcagat atgattgaag cgggaaagcg tgtgtatttc accaagtgcg tgtggtgcca
     1321 tggcgtggat ggagccggcg atggcccggg tgcagatcgg ctttggccgc gtccgagaaa
     1381 cttcaatcag ggaacattca aaattcgcca tacggcgagt ggagaattac cgcttttcaa
     1441 ctacaatgaa aagacccctg actccggcaa aaacgacttg ttcgatacgg ttactcacgg
     1501 tctccccggc tctgcgatgc caccgtggga aggtatcttg accgaagaac agcggattca
     1561 agtgctgtcg tttgtgacta cgcaactggt gaaggaccgc aagtttacgg acaagcagtc
     1621 tgagacccaa accattcttc aactcggtac gattaagcca gccgaggcga ctgaagatag
     1681 catcaagaag ggtgcacagt tgatcgtgga taagaagtgc attgaatgtc acggcgtcga
     1741 aggccgcggt gacggcaatg ccttcaacct caaagacgac tggggattct ccattcagcc
     1801 ggccaattgg cacaagtgct ggaacttccg tggaagccgt caggatccgt acaatgttcg
     1861 gaacatcttc cgtaccttct ccactggcgt caacggaacc ccgatgccgt catttgcgga
     1921 cagcacgtcg atcgaagatc gttggaacat tgcgaacttc gtgaattctt tgtgcgaacg
     1981 cgatgaggaa gggaagcctc taggtatcga tcctctgact gacaagccga agatcaactt
     2041 cgtcattcca tccagtccag tagaaggcga aatttctagc gatcctgaga atgaaatgtg
     2101 gaagaagcag ggtcgtcggt atgtggccat gggtggtcag atcactcata agccacgcaa
     2161 ctttgtgaac cgtatcgacg acatctgggt caagtcactc tacaacgaca agagcgtggt
     2221 gtacttgatt cagtgggatg accggacgaa gagcatcgcg gaaggcaagc ttccttgggc
     2281 tccgacccaa gttaacgtcg aaaacttcgg cgtgaaagag caggctccaa agaccggcga
     2341 agaaggttcc atcgcagcgg cgcagaataa ctatgccgtc tataacgatg gtattgcatt
     2401 ccagttcccc atcaagtggc aggaaattcc tgctcccttt aaaccacgct atttgttcgg
     2461 cgatgcgaaa ttcaatgccg acatcttgaa gtgggaagcg gacggttcgc tgcgttcctt
     2521 caagggcacg ggatgggatc aggattttga agagcgtgat gacttcgaag agaaggttaa
     2581 gcttttgaag tcagagtgga aaaacggtca gtggaccgtc atgatctccc gtccgctcaa
     2641 gggtagcaag gatgattatg acgaatacac ccggtttgat atcggtaagt acatcccgat
     2701 ggtattcttt gcctgggatg gacacaatgg tgatgcaggt cgaaagatgg cagtctctgc
     2761 attctactac acgattctgc agccaccgat tcctcaggaa gtgtacattt acccggccgt
     2821 tattgcagtt ggcgtcgtgt tcttggaggg ttggatgttg acccgccgcg ccaacaagaa
     2881 gaagggcaag gtcctgtaag cggtaaaagg gtaatgtagc tctcataagg cgagggggtg
     2941 aggaaactca ccccctcgtt gttttttagc tgttgcatta gatgtgagaa gtgggtccga
     3001 tgatagatga tatttcaggt gtcttagtcg ccggcggtcg aagtaggcga atgggacaag
     3061 acaagcgatt catgaaggtt ggtggcgcaa gtgtgtttga acgaacgctg tccgtcttaa
     3121 gggttacctt tgcggaaaac attgtagtgc tcgcggagcc gatagagggg cttgatgtgc
     3181 agaggtgccc agttgtctat gacctgatcc caaacgcagg tagcttaggt ggcctgtata
     3241 ccggtttgat gaccgcatca cgatcgaggg tatttgcctt agcgtgtgac atgccgtttc
     3301 tagaccctga agttatccga ttcatgctgt cccaggatga gtcggccgat gtgatcgtgg
     3361 ctaatttggg aggtcgattt cagccaatgc atgcggtgta ttcgaagcgc tgtgtcccgt
     3421 ttctccaggc gatggctcaa cgaaatgaac tcaagattca aaacctcttt caccagaacg
     3481 ccctgcgaat gaagatattg acagagaatc atctccaggc attcggacca gggttccgtt
     3541 cgttccagaa tatcaacacg ccgagtgagt tggcttctgc cgaggcatcc ccttctattc
     3601 acccgtgact gctcgtgcct ataatcgccc gcctctacga ggggtgttgg tcattcaccc
     3661 aggggctttg ggcgatgtgt tgttagcccg tcctgttctt cgcgcgttgc gttgtcaatt
     3721 ccctcaacat gaaatcgcac tgatagccgc caatgctgtg ggatctctgt tgtgccacag
     3781 cgcagagctt gatcgagcat tctctcttga ggaaaattac ctgagccagt tgtttgctgg
     3841 cttcgagagg ctgagtgatt cattcaggac ttggttgggg cagtgcgagt tagtggtggg
     3901 atggctgagc gatgccgatg gtgccgtgac caatacgttg cgcctcgcag gggtacagca
     3961 gattcagtta cagactccgt tttcttcggc cttagcgaca actcatcaag cagatcgcta
     4021 ttgggaagca gtcggaatgg agggacagcg ggaagacttc acctcatcgc taatgttgcc
     4081 cgccgaactg gaaaggcagg gccaggatgt cttgcaggca tatgactggg acggatcgtc
     4141 accattgatc ctggttcatc cgggaagtgg tagccataac aagtgtatgg atgcgtggcg
     4201 ccttgcaaag gtcattgagt ggttgatcga ggcgcgtatg gctcccatgc tcctggaagg
     4261 gccagctgat tgccgggccg tggcgcaggt gctttcatct ctgactgtgc cggtgcccgt
     4321 cattcgcgag ctcagtttgt ctgtcgtagc cggagtcatg tcacaggcga agctttatct
     4381 tggccacgac tcgggcatga cacatcttgc tgccgcgtta gctgttccca ccatagcgtg
     4441 ttttggtcct actcatgctt ctcgctggtc tccgcagggg cgcgcggtag cggtcatttc
     4501 cggtatgcca tgcgtttgtt ctgattggag caaggtggag acttgccacg acaaggtgtg
     4561 tctgcacatt cctgtggaaa gcctgattga agcctgccgg agtcaattgc tcaggttgtc
     4621 atagtccttc tcgacgatct cctcatcttg tctctcacat tctggtgtgt tagattgcga
     4681 gattaatttt cgctgtttcc acgcagactt aggggagttt ttgtggctca gggtgtggtg
     4741 caagataggg tagcagacgc gttggcagga gcgcttcgat cggcacaaca acgcgggatt
     4801 ctcaagatcg agcagatgcc gactattaac ctcgaggcgc caaagcggcc tgagtggggc
     4861 gatgtgtcct gcacggtggc catgtctttg tcggcttccg agcgacggcc tccatttgaa
     4921 attgctcaaa ttatcgcgga tcacattcag gatcgagatg cactgttctc gcgggtcgat
     4981 atcgcgcggc ctggatttct taatctcacg ctccaacccg atctctggat ggaagttctg
     5041 cgacatattg aaacccagcg ggccgagtac ggtcacagcc gactggggca gggccggcgc
     5101 gtattggtcg aatacgtcag cgccaatccg actggtccct tgcatgtcgg ccatgggcgt
     5161 ggagccgccg tggggcaggc gctcgttcgt ctcctcaggg caaccgggca tgaggttgtc
     5221 agcgagtatt atatcaacga tgcgggccgt cagatgaaat tgctgggggt gtcagtcctg
     5281 gcccggtacc tcgaatcgtg cggacaggca gttccctttc ccgaagacgg gtatcaaggt
     5341 gagtatattc gggccgtcgc tgcgcgtgtg aaggccgagc agggagtaac gctgctctct
     5401 ctttcctcga gtgaggcaga gcagcgaagc aaggatttcg cctatcggga acttctggcc
     5461 ctgatacggc aggatttgga aacctttggg attacgtttg agtcctggtt cagtgaagct
     5521 tccttgttgt cctctggagc cgtcgagcag gttttgagcg agctgagaag tcgggacctt
     5581 ctcttcgacc aggatggcgc gcaatggttc cgctcgtctg cctatgggga tgaaaaagat
     5641 cgggtggtgc gcaagcagga gggagattac acgtacttgg cctcagacat tgcctatcac
     5701 cgcgacaagc tgcggcgtgg gtttgatctc ctggtcgatg tctggggagc cgaccatcac
     5761 ggatatattc cgcggatgca agcggtggtg caggcctacg ggtatcccaa ggaacggttg
     5821 cgcgtggtgc tggtgcagat ggtcaacctt ctccgcggcg gccggaaagt ggaaatgtcc
     5881 aagcgagccg gagagtttat tacactccgc gaggtcatgg atgaagtcgg ggcggacgcg
     5941 gccaaatttt tctttctcat gcgcgactcc agcacgcatt tagattttga tctagagctg
     6001 gccaagcagc agtcgcagga gaaccccgtg tattacgtac agtatgcgca cgctcggatc
     6061 agcagtctgt tgcgcgtggc ggcatcacga ggcattgagt gtccgcttcc gagtcaggct
     6121 gacctcaaac tgctgaacga tcccgacgaa ctcgcgatca tacgaaaact ttcggtcttc
     6181 ccggtggtgc tggaagcgag cgctgtcgag ctggagccgc atcggatggc ctactatctt
     6241 cgtgagttgg cgggcctggt gcatcctttc tacaacaaac accggatatt gcctccgatg
     6301 accgacctcg acgcgactcc ggcggctcca accgagccgt cggcttctac cagtcgggtg
     6361 cccgaggcgg tgtcgcccgg gctgacaggg gctcgcctgg ccctcatgtg gtcggtgcag
     6421 caggtggtcc gcaacgggtt gacggtgctg ggtgtttcct ccccagagca gatgtaggct
     6481 gccaatcgga tgctatcgtt tcgcattacc catgaagaag caggcgggcg ggcgcgggtc
     6541 ggggttctca ccaccggacg tagcgaagtg gcgacgcccg ctttcatgcc ggtcggttcg
     6601 ctgggcaatg tacgtagtgt tgacggtgaa gaactgctga agatggggta tgggctgatc
     6661 ctgaacaatg cctatcatct ctatctgcgc cccggccaca aagtcgtgga ggagttgggc
     6721 ggcgttcatg gcttcacggc ttggcccggc gccattctca ccgacagcgg tggcttccag
     6781 gtctttagcc tggcaaagtt ttgcaaagtg acggatgagg gcgtggtctt tcaatctcat
     6841 atcgatggat cgtcacgatt catcagcccc gaaacgtcga ttgagattca ggaagcgttg
     6901 ggcgcggaca tcatcatggc cttcgaccac gtcgtcgctc tgccctcctc gctcgaccag
     6961 gtgcgtgccg ccgccaagcg cacatctttg tgggcgcgcc gctgtgtcga ggccaagcgt
     7021 cgtactgatc aatcgttatt cgggattgtg caaggcggat tggaccagag cctacgagtg
     7081 gagtctgccc gcgatctggt gtcggtggga tttgacggat atgcggttgg cggcttgtcc
     7141 gttggcgaag acaaggccga catgtatgcc atgttggatg tcacggtccc cgaactgccg
     7201 tcggccaagc cccgctattt gatgggtgtg ggcatgccag aggacttggt ggaaggcgtc
     7261 gcgcgcggga tcgatatgtt cgattgtgtg gtcccgtccc gccatggccg aaccggatgg
     7321 ctgttcacct ccttcggacg tgtcgtgatc aaacaggctc gatatgcgag ggatgaacag
     7381 cccatcgatc cggcctgccg gtgtccggtg tgtacccgat atacgcgcgc ctatctccat
     7441 cacttgttcg gcgtgaaaga gatgttgggc gcccgcctca atacgattca taatttgtgg
     7501 ttttttgcga agttgatgga agagatccga aacgccgtgc ggagcggcac ctttcaggag
     7561 tttcgccgtg agtttcaccg gacctatgtt gtggaccggc cgggcggcga agggcggtcg
     7621 gacgtagaca atacgaacat gtcgtaaggc gacaagcagg gggctgtatg tgggattcgg
     7681 tggcctgggc gcagggaacg tcgggaaacg cgggagcggg gggaggcctg ttatcgctgg
     7741 tcccgttcgt gctgatcttt gtcatttttt atttcatgct gattcttccg caacaaaaac
     7801 gccagaagca ggcgaaggcc atgctggaga ccttgaagaa gggtgacaag gtggtgacgg
     7861 cctcaggcat ttggggcacc atcacgaacc tgggaaaaga gacggtgacg ctccaaattg
     7921 cggatacgac caaaatcaaa atccaaaaag agcatattgc tcggctgcgc ggtgaagaag
     7981 aagactgacg cacgcgacgg agacgggaag gattggcgca cgtatgaaaa aagtcggcgg
     8041 acggttgtta gccttagtga caatggtggt cgcttcgatc atctttttct tgccctccta
     8101 ccaacccctc tataaggaat tgccgcggtg ggtccgcgag gtgttgccgg ataagggcat
     8161 tacgctggga ttggacttgc aaggcggcat tcacctggtg ttggaagtag aagaagatcg
     8221 ggctgttgag attgcggtgg aacggacggc ggcgggactg caagatctcc tcgtcgaaaa
     8281 gaaaattccg gctgaatcgg tcaaacggac gggatcttcg caaatcacca tcggatttca
     8341 aaatgccgaa ttgaaggccc aggtacagaa gctcctggat gagttcccta cgtatgtgga
     8401 gaacgagggc gcgggctctg ggaacaaagt cgtgtgggaa ttgcgcgagg ccgagattaa
     8461 gcgcatcaaa gattccgcca tcaatcaggc cctggaaacg attcggaatc gcatcgacca
     8521 gtttggtgtg gcggagccgt tgattcagcg gcaggggttg aagcaagtcg tcgtgcagtt
     8581 gccaggcatc aaagacgcca aattggcaaa ggacctcatc aagcagaccg cactgctcga
     8641 attcaaactg ctggatgacg acaatcaact caagctggat ttgccgggcc ggatccaaaa
     8701 gggtaaggaa cgtgaagagg cctttctgaa gcaggttgaa gcaaaggttc cggaaggcga
     8761 tcaaattctg tttgagcgga tcgtcgaaaa ggagagtggt caagaatggc tggcccccta
     8821 tctggtgaag aaacgggtca tgctggcagg cgatgtgctc agcgatgccc gggtctccat
     8881 cggccagttc aatgagccgt atgtgtcggt gacgttcgat gcaaaggggg cacgggagtt
     8941 cgatcggatc acgggcgaaa acgtcaagaa gcg
//
LOCUS       DAOQEW010000153         8929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2595_length_8929_cov_8.310119, whole genome shotgun sequence.
ACCESSION   DAOQEW010000153 DAOQEW010000000
VERSION     DAOQEW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2595_length_8929_cov_8.310119"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLS35_14630"
     CDS             <1..798
                     /locus_tag="PLS35_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNI19955.1"
                     /translation="LVNMQGKLIGINTAIFSRTGGSEGIGFAIPSSIATDIVDSLTKT
                     GKVVRGWMGVAIQEITPALAKSFKLPEQRKGVLISDVNENGPSHTAGMRRGDVVISFN
                     GKEVQSVSQLRNLVARMGVGKDADIKILREGKEQVLKVKVAERPSDEVLAKREPAPSA
                     APAETVKPPDNVLAALRVQLLDAAMQSQLNIPAKTTGVVVSSVEAGSAAESAGLQRGD
                     VIQEVNHEVVKSLEDYQKASAKVKKDEMVVLLLSRQGNNLFVAVNPK"
     gene            890..1837
                     /gene="tatC"
                     /locus_tag="PLS35_14635"
     CDS             890..1837
                     /gene="tatC"
                     /locus_tag="PLS35_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNI19956.1"
                     /translation="MADGFKFQEWLQDTVFKPLEDKKMPVMEHLVELQVRLTRAVIVT
                     AIIFVGTFFYADTLVKWIRIPLQNMFVPGSLSWIPTDLPTVPFVFLAPAEALWQNVKV
                     AGLFALVLGTPYILVEFWHFVVPGLHAQERRFVGPFVILSTLAFYLGLLFSFFFVLPF
                     ALNFLISYGVNAGFIPQLSIAQYVGFALWFLLVFGLIFEVPLVITLLAKLGWVDAPLL
                     KRYRKWAFLTAFIFAAILTPTPDPFNQCLMALPMYIFYEVGIVSAGIFNKKKADAPAE
                     PLVPAVVAAGPGLAKAGPTIPSGGGDDEYVGVPSGGRRG"
     gene            1919..2875
                     /locus_tag="PLS35_14640"
     CDS             1919..2875
                     /locus_tag="PLS35_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNI19957.1"
                     /translation="MPRELNVISQPGSSSGGDACTYMWACAICDENETCQKDKEGHSR
                     WLVAKRMERIEYKVLVMSNKGGVGKSTCTTNLAVSLALKGWHVGICDMDIHGPNIPKM
                     VGAEGQKLKISTSGGIIPFQAYNMKIASMSFLLQNSDDPIIWRDAYKYEFINQLLGGV
                     EWQDLNFLLIDLPPGTGNESVTTIDLLGGVSGAVIITTPQEVALLDSRKSVTFCKDSE
                     VPIIGIVENMSGLECPHCHKEVNVFRKGGGEASAADMGVPFLGRIPLDPDVVTQSDAG
                     EPFALFNSDAATAQAYHDIANQVEAFCKKSGSLIKPAPRQQH"
     gene            2890..4188
                     /locus_tag="PLS35_14645"
     CDS             2890..4188
                     /locus_tag="PLS35_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNI19958.1"
                     /translation="MKSADATVGLTPLHEAQRIVLDAAAPLGLEKVSIMDTLGRVLGE
                     DIIATRSNPPWDNSAMDGFAVRAEDIAQEHAISKPVTLTVIEDVPAGKMPTKSVGKGQ
                     AIRIMTGAPIPAGADTVIKVEDTEHTPDSVRVFKAEPRGSNIRPQGEDVQKGDCIIPK
                     GTQIRSGEAGMLAILAKSFVLVYQRPRVAILSTGDELADLDERFSEEKIINSNSYGIA
                     AAVQEAGGVPILLGIARDTPAALTEKISHGLNADILVLSGGVSMGDYDFTKAVFRDLG
                     AEMNFWKLAIRPGQPLAFGKIQGKLAFGLPGNPVSSMVTFEQLVRPAMLKLSGARSYG
                     RPLLEAVFQEKFSKRTDRRHFLRGVLWREDGVFKVRTTGDQGSGILTSMVKANCLIDI
                     PVEVERLNPGDPVTVQLLSGSVWLDQAAPAASAAHRPSCC"
     gene            4210..4719
                     /gene="mobB"
                     /locus_tag="PLS35_14650"
     CDS             4210..4719
                     /gene="mobB"
                     /locus_tag="PLS35_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNI19959.1"
                     /translation="MSVPILSFVGRSNSGKTTLIERVIPELVRAGYKVATVKHAGHGF
                     DLDTEGKDSWRHKQAGASSVVIISKSSLAMFADVSDHMNVEDVRERYLDPSYDLILAE
                     GWRSEGYPKIVVVRDQIGEVPVSQDGLLAVVSNKPVETSVPLLDPDDVAAVAALIIRH
                     FPKSQRDDA"
     gene            4709..5416
                     /locus_tag="PLS35_14655"
     CDS             4709..5416
                     /locus_tag="PLS35_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNI19960.1"
                     /translation="MMRSTPAIVFGAILAGAVAFGGVAVPLTNHPKFCASCHTIQPAY
                     EGWLQSSHKEVECVACHVRPGVVGWLHDKAWHGTRDVAIYLFGKPTEPHNLQAQVDSA
                     VCLGCHRNILRVSEIAPRDLPSPVKDVGLIMSHRKHMAAFDQRAQGEGCTTCHAAVVH
                     ERPIKGYPIVIPRGHVSADSRPWNSTHPEGSVLHKRAQADCFRCHDNKTEYEGRVLSR
                     KCETCHLPEKLSEFLSF"
     gene            5436..6161
                     /locus_tag="PLS35_14660"
     CDS             5436..6161
                     /locus_tag="PLS35_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19961.1"
                     /translation="MSSPVVEVRNLSKRFGGFTAVDGISFEIGRGEILGLLGPNGAGK
                     TTTFQMMLGLVTPTAGSIRMFGLDLQSNRETILQQVNFSSTYISLPFSLTVEENLRVV
                     ARLYGMSDIAARVDDMVKKLEMGDWRHKLTRKLSSGQMTRLTLAKALLTEPKVLFLDE
                     PTASLDPDIAHKIRAILLEERQATGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     AKDIVARFGQADLEDVFLKLARE"
     gene            6209..6685
                     /locus_tag="PLS35_14665"
     CDS             6209..6685
                     /locus_tag="PLS35_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19962.1"
                     /translation="MVDAGISFTVGVLIGGLSLMVGWGLLWFVVGGIGLARQTSGWPI
                     FISSISSTAIAGGVMAVVFWAVDAARLSSPALHVGLAGIPLALLAASAFRLEDGRRIG
                     PAFVQGSRLMMQELLGGHQEEGGCGHCHEQPVEEHPCHQKPCFEQSCHEKPCEGKL"
     gene            6692..7483
                     /locus_tag="PLS35_14670"
     CDS             6692..7483
                     /locus_tag="PLS35_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI19963.1"
                     /translation="MAYHRIAALVIRHLYLYRRSLPRVMEIIYWPFLDLVVWGFITVY
                     LATFQGQMPAVVTFLLGALILWDVLFRSQQGITISFLEEIWARNLMNLFASPLTPSEF
                     LAATMVMSLFKVTAVSVVMSLCAWVFYGYNVFIIGLWLMPFIANLVLTGWIIGVFTTS
                     LIMRFGQEAEVLAWSMVFLFQPISCVFYPMDVLPAWLRGVAAINPAAHVFEGMRGLLA
                     THAAPLTNLGMASGLNLLYLGAVVFWFHHTFNVCKERGSLVRVGE"
     gene            7524..8198
                     /locus_tag="PLS35_14675"
     CDS             7524..8198
                     /locus_tag="PLS35_14675"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HNI19964.1"
                     /translation="MKVVAGQVGQARSVAGLENFSFHKTPVLVIENFWSADERGQFRE
                     AMARANWNQLQEMSYVRQDFPNSGNWAKAEIAQPQGRLLLSRLELPCIQQYIESFPNI
                     TRRHMGFSYYSYGAGDCLLTHDDTDQGHPTSGRPAPRRRIALVSYFHEEWDCDWGGEL
                     MIYAPGGESKAGKPDLAITHCIAPKPGSLVMFTVPRFHRVCRVDQTAGDHKRLSIAGW
                     FMTEHA"
     gene            complement(8354..>8929)
                     /locus_tag="PLS35_14680"
     CDS             complement(8354..>8929)
                     /locus_tag="PLS35_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI19965.1"
                     /translation="EAGPAHDVVNHYLHAGHIQPCAEWPDLSQAPGNEVVRLRAVRAQ
                     TESGLIKDRFDIRRPIDLEVEFDVLKPGQVFVPVFNLYNEEDVTVFIAHDRDPAWQRT
                     PRPAGRYTSTARIPGNLLAEGMLTVSPLMMTEDPFRLHAHAPRALGFRVDDSADGDSA
                     RGDFHGRWPGVIRPLLEWRTRFEPVRDGRSG"
BASE COUNT         1809 a         2387 c         2746 g         1987 t
ORIGIN      
        1 ctggtcaaca tgcaaggcaa gttgatcggc atcaatacgg ccatcttctc ccgcaccggc
       61 ggatcggaag gcatcgggtt cgccattccc agcagcattg cgaccgatat cgtcgatagt
      121 ttgacgaaaa ccggcaaagt ggtgcgtgga tggatgggtg tggccattca ggaaattact
      181 ccggccttgg ccaagtcctt caagttgcct gagcagcgca agggcgtctt gatcagtgac
      241 gtgaacgaga atgggccgtc ccatacggca ggcatgcgcc gtggtgacgt ggtcatttcc
      301 ttcaatggca aggaagtgca gagcgtcagc caattgcgca acctggtcgc gcgcatgggt
      361 gtcggcaaag atgccgacat taagattttg cgcgaaggca aggagcaggt gttgaaggtg
      421 aaggtggctg agcgtccgtc ggatgaggtg ttagcgaagc gtgagccggc tccctcggcg
      481 gctcctgccg agacggtgaa gccaccggac aacgtcctgg cggccttgcg cgtccaattg
      541 ctggatgcgg cgatgcagag ccagctgaac atcccggcca agactaccgg tgtcgtggtg
      601 agttccgtcg aggcgggcag tgccgctgag tcggcgggat tgcagcgtgg cgacgtcatt
      661 caggaagtca accacgaagt cgtgaagagc cttgaggatt accaaaaggc gtcagccaag
      721 gtgaaaaagg acgaaatggt cgttcttctg ctgagtcggc agggaaacaa tctgttcgtt
      781 gccgtcaatc cgaagtaaga catggatgag ggaacgatcc gtcgagtgag cggggaattt
      841 ctcccgtact cactcgacgg agagtcctgc ctcaagtttg tgaacaggca tggctgacgg
      901 gttcaagttt caagagtggc tgcaagacac agtctttaag ccgcttgaag ataaaaaaat
      961 gccggtcatg gagcacttgg ttgagctcca ggtccggctg acccgtgcgg tcattgtgac
     1021 ggcgatcatt tttgtcggca ccttcttcta cgccgacacg ctggtgaagt ggatcagaat
     1081 cccgctccag aacatgttcg tgccggggtc cctttcctgg atccccacgg atcttcccac
     1141 cgtcccgttc gtctttctcg cgccggctga agccttatgg caaaacgtaa aggtcgcagg
     1201 tttgtttgcg ctggtgttgg gcactcctta catcctcgtt gagttctggc atttcgtggt
     1261 gccggggttg cacgcgcagg aacggagatt cgtcgggccc tttgtgatcc tcagcacgct
     1321 ggccttttat ctgggcctgt tgttttcgtt ctttttcgtc ctgccctttg ctttgaactt
     1381 tctcatctcg tatggcgtga atgccggatt tattccgcaa ctctcgattg cgcaatatgt
     1441 cggattcgcc ttatggttcc tgctggtctt cggtctcatc tttgaagtgc cgctggtgat
     1501 taccctgctg gctaagctag gctgggtgga tgctccattg ctgaaacgtt atcgcaagtg
     1561 ggccttcctg acggcgttta ttttcgccgc gattctcacc ccgacacccg atccgttcaa
     1621 ccagtgcctc atggcactgc ccatgtacat tttttatgaa gtcggcatcg tgagcgccgg
     1681 catcttcaac aaaaagaagg ccgacgcgcc ggcggaacca ctcgtgccgg cagtggtggc
     1741 ggcgggaccg gggctcgcga aagcgggacc aaccattccg tctggcggtg gagacgatga
     1801 gtatgtcggc gtgccgagcg gcggccggcg aggatgagca agaccggagt agagcaacag
     1861 ggcgaaagcg cgctttcgtc ttacgcagta tgatgttcgt tgtacgagga gttgaggaat
     1921 gccacgcgag ttaaatgtta tttcacagcc cggttccagc agcggcggag atgcctgcac
     1981 ttatatgtgg gcctgcgcca tttgcgacga aaatgagact tgtcaaaagg acaaggaagg
     2041 ccacagccgc tggttggtcg caaagcgcat ggaacggatc gaatataaag tgcttgtcat
     2101 gagtaataaa ggcggcgtgg gcaagagcac ctgcaccacg aaccttgctg tcagtcttgc
     2161 gctgaagggc tggcatgtcg gtatttgcga catggatatc catggcccca atattccgaa
     2221 gatggtcggg gcggagggac aaaagctgaa gatcagcact tcgggcggga tcatcccgtt
     2281 tcaggcctac aacatgaaga ttgcctcgat gtccttcctg ttgcagaact ctgacgatcc
     2341 catcatctgg cgcgatgcgt ataaatatga attcatcaac cagttgctgg gaggcgtgga
     2401 gtggcaggat ctgaatttcc tgctgatcga tctgcctcct ggaacgggca atgagtccgt
     2461 gacgaccatc gatctcctcg gtggggtcag cggagcggtc attattacga cacctcaaga
     2521 agtggcgttg ctcgattccc ggaaatccgt gacgttctgc aaggacagcg aagtgccgat
     2581 tatcggcatc gtggagaaca tgagcgggtt ggaatgcccc cactgccaca aggaagtgaa
     2641 tgtcttccgc aagggcggag gtgaggcctc ggccgcggat atgggtgttc cgttcttggg
     2701 acggattccg ctggatcccg acgtggtgac gcaaagtgat gcgggcgagc ccttcgcact
     2761 gttcaactcc gatgcggcaa cggctcaggc ctatcacgat atcgccaatc aggtggaagc
     2821 tttctgcaag aaaagcgggt cgctcataaa gccggcgccg cgacagcaac attgacggga
     2881 cggtgacctg tgaaatctgc cgatgcgaca gtcggattga cgccgcttca tgaagcccag
     2941 cgcattgtgc tggacgccgc ggctccctta gggctcgaga aggtctccat tatggacacc
     3001 ctgggccggg tgctcggcga agacatcatc gctacgcgct cgaatccgcc gtgggacaat
     3061 tccgcgatgg acgggtttgc cgtacgcgct gaggacattg cgcaggaaca cgcgatttct
     3121 aagcctgtca cgctaaccgt cattgaggat gtgccggcag ggaagatgcc caccaagtct
     3181 gtgggaaagg ggcaagcgat cagaatcatg acgggcgccc ctattcccgc cggggctgat
     3241 accgtcatca aggtcgagga tactgaacat acgcccgatt ccgtgcgtgt gttcaaagcg
     3301 gaaccgcgcg gctcaaacat ccggccgcag ggtgaggatg tgcaaaaggg cgactgcatc
     3361 attccgaagg gcacccagat tcgatccggc gaagcgggta tgttggctat tctcgcgaag
     3421 tcctttgtgc tggtctatca gcgtccgcgc gtcgccatct tgtcgacggg cgatgaactc
     3481 gccgatcttg acgaacgctt cagcgaagaa aaaatcatca attcgaacag ttacggtatt
     3541 gctgcggcgg tgcaagaagc cggcggcgtg ccgatcctgc tgggcattgc tcgggatacg
     3601 cctgcggcct taacggaaaa aatttcccac ggattaaacg cggacattct cgtgctatca
     3661 ggcggcgtgt cgatggggga ttacgacttc acgaaagcag tattccggga cctgggtgcc
     3721 gagatgaatt tctggaagtt ggccatccgg ccaggccagc cgctcgcctt cggcaaaatt
     3781 cagggaaagc tggcgttcgg actgccggga aacccggtct cttcgatggt gacctttgag
     3841 caactggttc gtccggccat gttgaaactg tccggcgcgc gaagctatgg acgtccgttg
     3901 ctcgaggcgg tctttcagga gaagttttcc aagcgaaccg atcggcggca ttttctgcgc
     3961 ggggtgttgt ggcgggaaga tggagtattc aaggtcagga cgaccggcga tcagggctcg
     4021 ggcattttga cctccatggt caaagcgaac tgtttgatcg atattccggt cgaagtagag
     4081 cgcctcaatc ccggtgatcc ggtaaccgtg cagcttctca gcgggtcggt atggctggac
     4141 caggccgctc cggctgcttc tgccgctcac cgcccctcct gctgctgacg ccgcgaaagg
     4201 aacgttgtca tgtccgtgcc cattctgtct tttgtcggcc ggtccaatag cggcaagacc
     4261 acgttgatcg aacgcgtgat tcccgagctt gtgcgtgccg gctataaggt ggcgacggtc
     4321 aaacatgccg gtcacgggtt cgatttggat accgagggaa aggacagctg gcggcacaag
     4381 caagccggtg cgagcagtgt cgtgattatt tccaagagca gtctggccat gttcgccgat
     4441 gtctcggacc atatgaacgt cgaggacgtt cgtgagcgat acctcgatcc ctcgtatgat
     4501 ctgatcctgg ccgaaggatg gcggagcgaa ggctatccga agatcgtcgt cgtgcgcgac
     4561 cagatcggtg aggtgccggt ttcgcaagac ggcctcctag cggtcgtgtc caacaaaccc
     4621 gtcgaaacct ccgtcccgct gctggatcct gatgatgtgg cggcggtggc ggcattgatc
     4681 atccggcatt tccccaaatc ccaacgggat gatgcgtagt actcccgcca ttgtgttcgg
     4741 ggcgattctg gccggcgcag tggctttcgg gggagttgcg gttccgctga cgaaccatcc
     4801 gaaattctgc gccagctgcc ataccatcca acccgcctat gagggctggt tgcaatcatc
     4861 ccataaggaa gtggaatgcg tggcctgtca tgtcaggccc ggtgtggtcg gctggttgca
     4921 tgataaggcc tggcatggga cgagggacgt ggcgatctat ctgttcggca aaccgacgga
     4981 gcctcacaat ctgcaagctc aggtggattc cgccgtctgc ctcggctgcc accgcaatat
     5041 cctgcgggtg tcggaaatcg cgcctcgtga tttgccgtct cccgtcaagg atgtggggct
     5101 gatcatgagc catcggaaac atatggcggc gtttgatcag cgtgcgcaag gcgaagggtg
     5161 taccacctgc cacgcggcag tggtgcatga acggccgatc aaaggatatc caatcgtcat
     5221 tccccgaggc catgtgtcag ctgatagccg tccgtggaat tccacacatc ctgagggctc
     5281 ggtgttgcac aaacgggcac aggcggattg ctttcgatgc cacgacaaca aaaccgaata
     5341 tgaaggccgg gtgctgagcc gaaagtgcga aacgtgccat ctgcctgaaa aactctcgga
     5401 attcctgtcg ttttagccca cggaaccagc tgcccatgtc atcgccggtg gtcgaagtac
     5461 gtaatctcag taaacgattc ggtggattca ccgcggtcga cgggatttct ttcgagatcg
     5521 gccgagggga aatcctcggc ttattgggac ccaatggcgc cggcaaaacg acgacgtttc
     5581 aaatgatgtt agggttagtc acgcccacag ccggctcgat ccgcatgttc ggtctcgact
     5641 tgcagtcgaa tcgggaaacg attcttcagc aggtcaattt ttcctccacc tacatttcat
     5701 tgcctttctc cctgacggtt gaggaaaatc ttcgagtcgt cgcccgattg tacggcatgt
     5761 ccgatatcgc ggcccgtgtg gatgatatgg taaagaaact ggagatgggc gactggcggc
     5821 acaagctgac gcgcaaactg tcgtcagggc agatgacgcg tttgacgttg gcgaaagctc
     5881 tcctgacgga acccaaagtg ctgtttttgg atgaaccgac ggcgagtctg gatccggata
     5941 tcgcgcacaa gattcgcgcg atcctgttgg aggaacggca ggcgaccgga ctcagtattc
     6001 tttacacctc gcataatatg cgggaaatgg aagagatgtc ggatcgcatt atttttctgc
     6061 agcggggaaa gattgtggcc gaaggcaccg ccaaagacat cgtggctcga tttggtcagg
     6121 cggacctgga ggatgtgttt cttaaactcg cgcgcgagta gcaggcccgc gcgggatcgt
     6181 gtgatgtgca aggggagcag gcagcgccat ggttgatgca ggaatatcgt ttactgtggg
     6241 ggtactcatc ggcggcctga gcctcatggt gggatggggc ttgttgtggt tcgtcgtggg
     6301 cgggatcggc ttggcacggc agaccagcgg atggccgatt tttatcagca gtatcagcag
     6361 caccgcgatt gcgggcggcg tcatggctgt ggtgttctgg gctgtcgatg ccgcacgact
     6421 gagcagcccg gcgttgcacg tcggtctggc agggattcca ctggccttgc ttgcggcgtc
     6481 agcctttcgc ttggaagacg gccggcgaat tggtccggcg tttgtgcaag ggagccgctt
     6541 gatgatgcag gaactactcg gcgggcatca ggaagagggc ggatgcggcc attgtcatga
     6601 acagccggtt gaagagcatc cctgtcacca gaagccgtgc ttcgaacagt cctgccacga
     6661 gaagccttgc gaagggaagc tgtagccggc gatggcctac caccgtattg cggctttggt
     6721 catccgtcac ctgtatctct accggcgcag cctgccgcgg gtgatggaga tcatctattg
     6781 gccgtttctc gatctggtgg tgtggggctt catcacggtc tatctggcga cgttccaagg
     6841 gcagatgccg gcggtcgtga cgtttctgct cggtgcgctg attctctggg acgtgttgtt
     6901 tcgatcacag cagggcatta cgatttcctt tcttgaggaa atttgggcgc gcaacctgat
     6961 gaatctgttc gccagtccgc tgacgccctc cgaatttctc gcggctacga tggtcatgag
     7021 cctcttcaag gtcactgccg tgtcggtggt catgtccctg tgcgcctggg tgttttacgg
     7081 ctacaatgtt tttatcatcg gcctgtggct catgccgttc attgccaatc tcgtgctaac
     7141 gggatggatc atcggtgtgt tcacgacgtc gctgatcatg cggttcggac aggaggcgga
     7201 ggtgctcgcc tggagcatgg tctttttgtt tcagccgatt tcctgcgtgt tctatcccat
     7261 ggatgtcctg ccggcctggc tgcgaggcgt ggctgcgatc aatccggcgg cacatgtgtt
     7321 cgaaggtatg cgaggacttc ttgcgaccca tgcggctccg ttgaccaacc tcggaatggc
     7381 gagtggtttg aatctgctgt atctcggcgc ggtggtcttc tggtttcatc atacgttcaa
     7441 tgtctgtaaa gagcggggat cgttggtccg ggttggcgaa taattgatcg cgttcggcgt
     7501 gcgcgggttc gaggaggaga cgcatgaagg ttgtagcagg acaagtcgga caagcacggt
     7561 cagtagccgg gctggagaat ttttccttcc acaagacgcc ggtcctggtc atcgagaact
     7621 tctggtcggc cgacgaacga ggccagtttc gagaagcgat ggcgcgggcc aattggaatc
     7681 aactgcaaga aatgtcgtac gtccggcaag acttcccgaa ttccgggaac tgggcgaagg
     7741 ctgaaatcgc ccagccgcaa ggacggctgc tcttgagccg attggaattg ccctgcattc
     7801 agcagtatat cgaatcattt cccaacatca cccgccgtca tatggggttc agctactatt
     7861 cctatggagc cggtgattgt ttgttgacgc atgacgacac ggatcagggc catccaacga
     7921 gcggcaggcc tgcgcctcgt cgacgcatcg cactcgtcag ctactttcac gaggaatggg
     7981 attgtgactg gggcggggag ctgatgattt atgcgcctgg aggtgagtca aaggccggga
     8041 aaccggattt ggccatcaca cattgcatcg caccaaaacc gggatcgttg gtcatgttca
     8101 ccgtgcctcg ttttcatcgc gtgtgccggg tggatcaaac agccggggac cacaagcgcc
     8161 tctcgatcgc tggatggttt atgaccgaac atgcctagca ggatgttgaa aacgtctgcc
     8221 agtctcgttc tcgcaagaca ctgcaccctc accgtctcgg cggcgttcac aaacgtggtg
     8281 cgcggtattc agcggaacgg cctgtttgac cattccacgg gttacacacc aacatgccgc
     8341 ttcagtacga ccatcaacct gaccgcccat ctcgcaccgg ttcaaaccgc gttcgccact
     8401 ccagcagagg tctgatcacg cccggccatc gtccgtgaaa gtcacctcgc gccgaatctc
     8461 catcagcact gtcatccacc cggaacccga gagcccgcgg cgcatgcgca tgcaggcgaa
     8521 atggatcctc cgtcatcatg agggggctta cggtcaacat tccctcggcg agtaagttgc
     8581 ccggaatcct cgcggtggag gtataccggc cggcgggacg gggggttcgt tgccatgccg
     8641 gatcccgatc gtgcgcgatg aacacggtca cgtcttcttc gttgtagaga ttaaagaccg
     8701 gaacgaagac ttgccccggt ttaaggacat cgaattccac ctcgagatca atcggccgcc
     8761 ggatatcgaa gcgatctttg atcaaccctg actccgtttg ggctcgcact gcccgcagac
     8821 ggacaacctc attgcccggg gcttgggaca gatccggcca ttcggcacag ggctggatgt
     8881 gcccggcatg cagatagtga ttcaccacgt catgcgccgg cccggcttc
//
LOCUS       DAOQEW010000154         8859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2628_length_8859_cov_7.162881, whole genome shotgun sequence.
ACCESSION   DAOQEW010000154 DAOQEW010000000
VERSION     DAOQEW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2628_length_8859_cov_7.162881"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLS35_14685"
     CDS             complement(<1..209)
                     /locus_tag="PLS35_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNI19966.1"
                     /translation="MELGFVGLGKMGMNMVTRLQQGRHRVVAYDRAPDIVKQAEGKGC
                     IGAASLSDLVSKLTAPRAVWVMVPSG"
     gene            complement(365..1171)
                     /locus_tag="PLS35_14690"
     CDS             complement(365..1171)
                     /locus_tag="PLS35_14690"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNI19967.1"
                     /translation="MESHLATSSPQSSPALPSSEELKAELLDIPEPPEMPPAVTPPGY
                     DDALAGAVKYVRAKRGGDLVGIVLVGSGARRAVTAHSDIDLIVLVKGPADGQEMIRVG
                     DRLVDIRYGEHTTVAEDLTYSPRLAPLLRKGRVLYDHEDIAAQLIAKAAQRFRQGPPP
                     ATIHERIRLKAECLHWLGKVEDLRDKPNTAQYLLQLFFEECLSAFFRTRGLWFTAPVD
                     TLRFLASREPALGEVAAQFLSAATLHDRLMAGRRFAETLFRDIPLPPRVD"
     gene            complement(1190..1417)
                     /locus_tag="PLS35_14695"
     CDS             complement(1190..1417)
                     /locus_tag="PLS35_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19968.1"
                     /translation="MSLHAVSFQQISRILDVTDALGLNREWVEIPLSPETPGIVRKLP
                     NGKLEIVVDADQPFDAWLGTLEQQIRRVQAA"
     gene            complement(1495..2526)
                     /locus_tag="PLS35_14700"
     CDS             complement(1495..2526)
                     /locus_tag="PLS35_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNI19969.1"
                     /translation="MIDLRSDTVTKPSPAMREAMARAEVGDDIYGEDPTVNRLQEVGA
                     ALVGKRAALFVPSGTMGNQLSIRAQAEPGREVIVEAQSHVIRYEQGAAAALAGVQLHW
                     VTGTHGLMTAEQVQRAIRPKDPSSIQSALICLENTHNAGGGRVYPLTTIQAIRAVATA
                     HGIPMHLDGARLFNAVVASGVPAAEFAKHFETVTFCLSKGLGAPAGSLIATDDLSMME
                     RLRRFRRMYGGAMRQSGILAAAGLYALDHHIARLSHDHANAKRLAARLQQIPAVTIDP
                     SLVETNILFFEVHSTKFSIPAFVAALKHEGVLINAVGGATCRAVTHLDVSAEAIEEAA
                     DQIAHLLNK"
     gene            2647..3918
                     /locus_tag="PLS35_14705"
     CDS             2647..3918
                     /locus_tag="PLS35_14705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19970.1"
                     /translation="MERWYRSVIRFVQAEPQELRPLAWSFGYFFCLLCGYYILRPVRD
                     EMAIQGGVHNLPWMMTGTFLTLLAVTPLFGWLSARYSRYRLLLAVYLFFIANLIGLYF
                     LMTSGLYIEWVARGFFVWLSVFNLFVVSVFWSFMADLFTPAQGARLFGMIAAGGSTGA
                     LFGPLITTGLTYLFPVPVLMLASAAFLAACIGCIYRLEMWSRSRSLFHRENSGAPLGG
                     GFLAGVRLVWSSPYLLGICGYLTFLTMTATFLYFEQVRLVAQQFDTPEARTRLFSMLD
                     FATNILTWLTQLLLTNHVVARFGLAAALLFLPAVSLLGFLGIALWPGLVVYVSFSVLR
                     RVGEYALSKPAREVLFTVVSREEKYKAKNFIDTAISRAGDASTGWLVSGLKALSVTTA
                     QIAWVLVPLMVLWGWLGRWLAAQQRKQIGPG"
     gene            4100..5467
                     /locus_tag="PLS35_14710"
     CDS             4100..5467
                     /locus_tag="PLS35_14710"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNI19971.1"
                     /translation="MMVEVPVKLYLDKVLKTAREAVRPTSLLSGPIRDKALRGMATAL
                     AEAEESILAANETDVDAVGKSMTGYENRERVRDAVARIRMTADDVKALVDRLHHIADL
                     PDPLGEVLGRHEEPNGLQVSRVRVPIGVIGIVSELSPLETIDALALCLKSGNVCVFRG
                     SPDWTHTQHVIATALTKAMHDAGIPKGACTIIDRQEKEAALELIKSGKALDAIIPRGG
                     AGLRKVVQEQAKMPILCHDGGITHVYIDDDVEIPLAQNIVVNSKVQNPSAPNSVDTLL
                     VHQGIARPLLSALILRLLDEFKIDIYGCPKTVSLMGQMLMTGHKAVKPAQETDWNKQF
                     QGPSMAIKMVPGFDEALAHIAQHGPSHTCVIVTKSYESAMRFSKEVDAGSVLVNASSR
                     LNAGDSLGFGADIGLSSTRHHARGPIGLNQLTCEKYVVFGSGQLRHPHPVPLAYEDAI
                     MLKRP"
     gene            5506..6732
                     /locus_tag="PLS35_14715"
     CDS             5506..6732
                     /locus_tag="PLS35_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI19972.1"
                     /translation="MPADRNDDIDNALREHLAWWGLRHIESDAAYFAWQREVFTPEDL
                     AALHQSIEAKRAATDGASAEIAFYDLTAQARFIPALYSQRYEYYLEVGSRIAAQLPGA
                     QTILDAGCGIGLLTTFYAQRCPDATVVGIDRSSASVTRARQRAQELGLSNVRFECLDL
                     DQHELSGRYDLIVATHTLLQAEQDPGLPSRDWQDFNRALDAQAQSEFEQRTGVGLRLD
                     RLRNVLAGNGRMILFEKTRQLARRVPFQRALAARGLALHLPPEPTRYRSIEEVTDDGP
                     LYVTGLVSPSSALSWNEAPEPDGAPPLHVQQLRSLRVDPEQPLYENHEASAQQAWLDL
                     PAKRVVHQMTRKEADGRQLHVEWGRAGEFAYLYCANTFDQRQLVVVDQARAAMVETYY
                     KEIVGEQSTSQQRMMK"
     gene            6729..7580
                     /locus_tag="PLS35_14720"
     CDS             6729..7580
                     /locus_tag="PLS35_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19973.1"
                     /translation="MNDPREHSDLPPQVPLEVVGLVPAAGMAKRLQPFPCSKEVYPVG
                     FAMDQKSGLPRPKVAAQYLLEKFKTAGITKAFLVIKDGKWDIPNYFREGDLVGLSLAY
                     LVIAGSLGPPDTLDRAYPFLAQKRVAFGFPDILFGPDDAYARLIQRQEETGADVVLGL
                     HLIEDPRVWDMVDCDEEGRIHALVMKPASTTLTYGWCCAVWTPVFTDFLHRFLRADET
                     KRNLDRLASKANDPSGDLAVGIVLQAAMKAGLVMQSVKFPGARYLDIGTPENLLKAVR
                     EFGGKIG"
     gene            complement(7699..8172)
                     /locus_tag="PLS35_14725"
     CDS             complement(7699..8172)
                     /locus_tag="PLS35_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19974.1"
                     /translation="MSKRLLSVVLSLGLLGMPGCFSHISYQEAPVPTADQTVLYISRT
                     DSPPRLRKASIFINDKLVADLASKEFTWITIAPGSYILHVKWSPDLAISAENINLHIR
                     EKEAIFVKIDSATVLFLGGSVVKVRLSGGNHPDQITQVWPLNYVEPKALTLTPLE"
     gene            complement(8237..>8859)
                     /locus_tag="PLS35_14730"
     CDS             complement(8237..>8859)
                     /locus_tag="PLS35_14730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI19975.1"
                     /translation="ALRHLGALLLTQKRLDEAEPILKRSYETAQKELGDNHPQTAASL
                     STWAALHFAREHPEKAEPLYSQVIHIVERSAGKSHRELAVALTNHAKALFAMKQFAHT
                     EQQLRRAVAIYERQTGPSSIELANTLLSLAVVIGKQRLSDAEPLFKRGLTITESAYGP
                     DHPALAKALGLYAEFLRDQDRNAEADVLAERAESIRTRNMPERPTS"
BASE COUNT         1718 a         2517 c         2730 g         1894 t
ORIGIN      
        1 cccgacggaa ccatgaccca taccgcgcgg ggagcggtga gtttggaaac aaggtcgctc
       61 agggaggccg cgccgatgca gcccttgcct tctgcctgct tcacgatatc gggtgcacga
      121 tcgtatgcca cgacgcggtg acggccctgc tgcaggcgcg tcaccatgtt catgcccatt
      181 ttgccgagtc cgacgaatcc aagttccata ggtcaccctc tctggtgaag accgatatgt
      241 gatgactagt gcggagccgg ccaagaacgg agtggcgctc cagtggttcg gtgcaggtct
      301 gatgctatca cggtcggaca cccacgcaag cgctgtcgcc ttggcacggg gtgatggagc
      361 cctgttagtc cacgcggggt ggaaggggaa tatcgcgaaa cagggtctcg gcaaatcgtc
      421 ggccggccat caatcgatcg tgaagggttg ccgcgctgag aaattgggca gccacctcgc
      481 cgagtgccgg ctcgcgggac gccaggaacc ggagggtgtc taccggtgcc gtgaaccaga
      541 gccctcgagt tcgaaaaaat gcagagaggc actcctcgaa aaagagctgc agcagatatt
      601 gcgcggtgtt gggtttgtcg cgcaggtctt cgacctttcc gagccagtgc aggcattcgg
      661 ctttgaggcg aatgcgttcg tgtatcgtcg ctggcggcgg gccctggcga aatcgttgcg
      721 ccgccttggc gatcaactgc gccgcaatgt cctcatggtc gtagagcacg cgtcctttgc
      781 gaagcagggg tgcgagacga ggagagtagg tgagatcttc ggcgacggtg gtatgttcac
      841 catagcggat atcgaccagc cggtctccca cccggatcat ttcctgcccg tcggccggac
      901 ccttcaccag gacgatgaga tcgatatcgc tgtgcgccgt caccgcgcgt cgggcgccgg
      961 atccgacgag aacaataccc actaggtcgc cgccgcgttt cgcgcggacg tatttcaccg
     1021 cgccggcgag ggcatcgtcg tagccgggcg gcgtcaccgc gggaggcatc tccggtggtt
     1081 cgggaatatc cagtaactca gccttcagct cctccgagga cgggagcgcg ggggaagact
     1141 gcggggaaga ggttgccagg tgtgattcca tgtctatcct tcaacgcggt caggcggcct
     1201 gtacacgccg tatctgttgc tcgagggtgc cgagccacgc gtcgaacggc tggtcggcat
     1261 cgacaacaat ctcaagcttt ccattaggaa gtttgcgaac gatgccggga gtttccggcg
     1321 agagaggaat ttccacccac tcgcgattca gccctagcgc atccgtcacg tcgaggatgc
     1381 gactgatctg ttgaaacgac acggcgtgaa gcgacactca atcctctcta ctcttgttgg
     1441 cattgtaggt ggggggacag agactgtcaa cgtgaggcgc aaggcctgct cggtctactt
     1501 gttgagcaga tgcgcgatct gatcggcggc ttcctcaatc gcctccgccg acacgtcgag
     1561 atgggtcacc gcgcgacaag ttgcgccgcc gacggcattg atcagcacgc cctcatgctt
     1621 gagggccgcc acaaacgccg ggatggaaaa tttcgtgcta tgcacctcga agaacagaat
     1681 gttcgtttcg acgagggatg gatcgatggt tacggctgga atctgttgca gccgggccgc
     1741 aagccgtttg gcgttggcgt gatcgtgact gagtcgcgcg atgtgatggt ctaaggcgta
     1801 gagacccgcc gcggcgagaa ttccagactg gcgcatggcg cctccataca tgcgacgaaa
     1861 tcgccgaagg cgctccatca tgctgagatc gtccgtcgca atcagcgacc cggccggtgc
     1921 cccgagtcct ttggagaggc agaaggtcac cgtttcgaag tgcttcgcga attccgcggc
     1981 cggcacgccc gaagccacga ccgcattgaa caggcgggcc ccgtcgagat gcattggtat
     2041 cccatgggca gtggcgacag cgcgaatggc ctgaatggtt gtcagcgggt agacccggcc
     2101 gcctccggcg ttgtgcgtat tctcaagaca aatgagtgcg ctctggatgc tagagggatc
     2161 tttcggtcgg atggcgcgct gcacctgctc ggccgtcatg aggccgtgcg tgccggtcac
     2221 ccaatgaagt tgtacgccgg ccaacgccgc cgccgcgccc tgctcataac gaatgacatg
     2281 tgactgagct tcaacaatca cttcacgtcc tggttcagcc tgcgctcgaa tggacagttg
     2341 gttgcccatc gtgcccgagg gcacgaagag tgcggcccgt ttgccgacga gtgcggcacc
     2401 aacttcctgg agacgattga cggtggggtc ttcgccataa atgtcatcac ccacctcggc
     2461 gcgggccatg gcttcacgca tagcggggct gggtttggtg accgtatcgc tccgaagatc
     2521 gatcatggat gggcctcaat caggagtgga gtgtggttct tcaggacgaa ccgcgggtgc
     2581 attctagcaa aaattcttct tgccggtgtg ccggggcctg ctacactggc cccaattcgg
     2641 agccccatgg agcgatggta tcggtcagtc atccggtttg tgcaggctga gccgcaggaa
     2701 ctgcggccgt tggcctggtc gttcggatat tttttctgtc tcctctgcgg ctactacatt
     2761 cttcgccccg tgcgcgatga gatggcgatc cagggcgggg tgcacaatct tccctggatg
     2821 atgacgggca cgtttctcac cttgctcgct gtcacgccgc tgttcggttg gttgtctgcg
     2881 cggtattccc gctatcgatt gctcctcgcc gtctatctct tctttattgc caatctcatc
     2941 ggcctgtatt ttttgatgac gagcggtctg tacatcgaat gggtggcgcg cgggtttttt
     3001 gtctggctct cggtattcaa cctgttcgtc gtgtcggtgt tctggagttt catggccgac
     3061 ctgtttacgc cggcgcaagg cgcgcgcctc ttcggaatga ttgcggcagg cggcagcacc
     3121 ggcgcattgt tcggtcccct catcacgact ggcctcacct atctcttccc ggtgccggtg
     3181 ctgatgctgg cctcggccgc gtttctcgcg gcctgcatcg gatgcatcta ccgattggaa
     3241 atgtggagcc gcagccggtc tctgttccac cgagagaaca gcggtgcacc attgggcgga
     3301 ggctttctgg ctggtgttcg cctcgtgtgg tcgtcgccgt atctgctcgg tatctgcggc
     3361 tacctgacct ttttgacgat gacggctacc ttcttatatt tcgaacaggt gcgtctggtg
     3421 gcgcaacagt tcgatactcc ggaggcccgc acgcgcctgt tctccatgct cgacttcgcc
     3481 accaacatcc tcacctggct gacgcagctc ttgctcacca accatgtggt ggcccggttc
     3541 ggcctggctg cagccttact gtttctgccg gcggtgagcc tcctcgggtt tttaggcatc
     3601 gccctctggc cgggattagt cgtgtacgtc agcttctcag tgctgcgtcg agtcggggaa
     3661 tatgcgcttt cgaaaccggc gcgcgaggtg ctgttcacag tggtgagtcg cgaggaaaag
     3721 tataaggcga agaactttat cgatacggcg atctcgcgcg cgggggatgc ctcgaccggc
     3781 tggctggtct ccggccttaa agcgttgagc gttaccacgg cacagatcgc ctgggtcctg
     3841 gtcccgctca tggtcctctg gggttggcta ggccgatggc ttgcggcgca acaacgcaaa
     3901 cagattgggc cgggttaggc ctcccttcct ggcctctgtc gcctccagag atgggaccac
     3961 gctgctttga ctcacaaaaa aatcgcatgg tatagtccgg cgcggatcgt tgaaggcctg
     4021 taatgatgct gtcctgacga ttcccggcac cctgtgacca ttcaccggac gcccattcac
     4081 cagctcacca attggggaca tgatggttga agttccagtt aagctctacc tcgataaagt
     4141 attgaagacg gcgcgtgaag cggtacgtcc cacgtcgctt ctttccggtc ccatacgaga
     4201 taaggcgtta cgcggtatgg cgacggctct ggcagaagcg gaggagtcca tcctggcggc
     4261 gaacgaaacg gatgtggacg cggttggaaa gtcgatgacg ggatatgaaa accgtgaacg
     4321 tgtgcgggac gccgtggccc ggatccgtat gacggccgat gacgttaagg cgctcgtgga
     4381 tcgattgcat cacattgcgg atctccctga tccgcttgga gaggtgctgg ggcgccacga
     4441 agaaccgaac ggtcttcagg taagccgggt gcgggtcccc atcggcgtga tcggcatcgt
     4501 gtccgagctg tcaccgctgg aaacaatcga tgcgctggcc ctctgcctga agtcgggcaa
     4561 cgtttgtgtc ttccgcggct caccggactg gacccatacg cagcacgtga ttgcgaccgc
     4621 tctcacgaag gccatgcatg acgccggtat cccgaagggc gcctgtacga tcattgaccg
     4681 gcaggagaaa gaggcggcct tggaactgat caagtccggc aaggcgttgg atgccatcat
     4741 cccgcgtggc ggtgcagggc tgaggaaggt ggtgcaggag caggccaaga tgccgatcct
     4801 ttgccatgac ggcggaatta cgcacgtcta tatcgacgat gatgtggaaa ttcccctggc
     4861 gcagaatatc gtcgtgaact ccaaggtgca gaatccctcg gcgcccaatt cggtggatac
     4921 gctgctggtc catcagggaa tcgcccgccc gctcttgtcg gcgttgatcc tccgcttgct
     4981 ggatgaattc aaaatcgata tttacggctg cccgaaaacc gtgtccttga tggggcaaat
     5041 gctcatgacg gggcataagg cggtcaaacc ggcgcaggag acggactgga acaagcaatt
     5101 tcaggggccc agcatggcga tcaagatggt gccgggcttc gacgaggcgc tggcgcatat
     5161 tgcgcagcat ggtcccagcc acacctgcgt gatcgtgacc aagtcctatg agtccgccat
     5221 gcggttcagc aaagaagtgg atgcggggtc ggtcctggtc aatgcctcgt cccgattgaa
     5281 tgccggagac agtctgggat tcggcgcgga tatcggcttg agttcgaccc gccatcacgc
     5341 acgagggccc atcgggctca accaactgac ctgtgaaaaa tatgtcgtgt tcggcagcgg
     5401 gcaactccgg catccgcatc ctgttccgct ggcctatgaa gatgccatca tgctcaagcg
     5461 gccgtaacgc accgatcgtg attgctcttc cggcaagagg gcgttgtgcc tgctgaccgg
     5521 aacgacgaca tcgacaacgc cctgcgtgag catctcgctt ggtggggact ccggcacatc
     5581 gaatccgatg ccgcgtattt tgcctggcaa cgggaggtgt tcacccccga agatctggct
     5641 gccctgcacc aatccatcga agcgaagcga gcggcgaccg acggggcgtc cgctgaaatc
     5701 gccttctacg atttgacggc gcaggcccga ttcattcccg ccctctacag tcaacgctac
     5761 gaatattatc ttgaggtcgg ctcacggatt gccgcgcaac ttccgggcgc ccagaccatc
     5821 ctggacgccg gatgcggtat cggcctgctg accaccttct atgcacagcg ctgtcccgat
     5881 gcgaccgtgg tgggcatcga tcgttcgtct gcctctgtca cccgcgcgcg acagcgggcg
     5941 caagaattgg gactgagcaa tgtgcgcttc gaatgtctcg atctcgatca gcatgagctc
     6001 agcggccgct atgatctgat cgtcgccacg catacgctgc tccaggccga acaggatccg
     6061 ggccttccca gccgtgactg gcaggatttc aaccgagccc tggatgctca ggctcagtcg
     6121 gagttcgagc agcggaccgg ggtggggctc cggttggatc ggctgcgcaa cgtgctcgcc
     6181 gggaacggtc gaatgatcct ctttgagaaa accagacaac tcgctcggcg cgttcccttt
     6241 cagcgagcgt tggcggcacg tggcctcgca ctgcacctcc cgcctgaacc gacccggtat
     6301 cgttccatcg aagaagtgac cgacgacggt cctttgtacg tgacgggttt ggtttccccg
     6361 tcgagcgctc tgtcatggaa tgaagcgccc gagccggacg gtgccccgcc actgcatgtg
     6421 caacagttgc ggtcccttcg agtagaccca gaacaaccgt tatacgaaaa ccacgaggcg
     6481 tccgcgcagc aggcctggct ggatcttccc gcgaaacggg tcgtgcatca gatgaccagg
     6541 aaagaagccg atggacgaca gctgcatgtg gagtggggac gggcaggcga attcgcctat
     6601 ctctattgcg ccaacacgtt cgatcaacgt cagcttgtcg tggtcgacca ggctcgggct
     6661 gctatggtgg aaacgtacta caaggaaatc gtcggcgagc agtctacgag ccaacagagg
     6721 atgatgaaat gaacgatccg cgtgagcact ccgacctgcc tccccaggtg ccgttagaag
     6781 tggtaggcct cgtgccggcg gcgggaatgg ccaaacgcct gcagccgttt ccttgtagca
     6841 aggaagtcta tccggtcggg ttcgcaatgg atcaaaagag cgggctgccg cgtcccaaag
     6901 tggccgcaca gtatctgctg gaaaaattca agacggccgg aatcaccaag gccttcctcg
     6961 tgatcaagga cggcaaatgg gatatcccca attattttcg agagggcgac ctggttggtc
     7021 tgtcgctggc ctatctcgtg atcgccgggt cgctcggacc acctgatacg ctcgatcgtg
     7081 cctatccatt cctggcccag aagcgagtgg cctttggatt tccggacatt ctgttcgggc
     7141 ctgacgatgc ctatgcccgg ttgatccaac gccaggagga aacgggggcg gacgtcgtgc
     7201 tcgggttaca tctgattgaa gatccgcgcg tgtgggatat ggtggattgt gacgaggaag
     7261 gtcgcatcca tgctctcgtc atgaaaccag cctccacgac tctgacctac gggtggtgct
     7321 gcgcggtgtg gacgccggtg ttcacggatt ttctccaccg ctttcttcgg gccgatgaga
     7381 caaagcgcaa tcttgaccgg ctcgcgagca aggccaacga cccgagcggg gatttagccg
     7441 tgggcattgt gctgcaggcc gcaatgaagg cagggctcgt gatgcagagc gtgaaatttc
     7501 ccggcgcgcg ctaccttgac atcggcacac cggagaattt gctcaaggcc gtgcgggagt
     7561 tcggcggaaa aataggttga tgcagattgt tctcgctagt tgcggcgttg cggactgtgc
     7621 ccagtgagca gatcggtagc tgaggacgag caacagctag attctgtagg tatacagccc
     7681 actcttttcg tcggcggatt attcgagcgg cgtcaatgta agtgccttgg gttccacata
     7741 attcaacggc cagacttgcg tgatttgatc cggatggttg ccgccagata gccgaacctt
     7801 cactacactc ccacctaaga acaagaccgt ggccgaatcg atttttacaa agatggcttc
     7861 tttctcgcgg atgtgcagat tgatattctc agccgagata gccaaatccg gtgaccattt
     7921 cacgtgcagg atgtacgaac caggtgcaat ggtgatccag gtaaattctt ttgaggcgag
     7981 atcagccacc agcttgtcat tgatgaagat ggaggcttta cgtagcctcg gcggagagtc
     8041 ggtccgagaa atgtagagca cggtttggtc tgctgtcggt accggagcct cttgataaga
     8101 aatatgggag aaacaccctg gcatccccag cagaccgagt gacagtacga cactcaataa
     8161 cctttttgac atagaacctt tattcctgca tcccgcgagg ggtgaccgta tctcgcatct
     8221 gaccaagaag tatgcattag gaggtggggc gctcgggcat attcctggtt cgaatcgact
     8281 cggcccgttc cgcgaggaca tcagcctcag cattcctgtc ttgatcccgg agaaactctg
     8341 catagaggcc caaggctttg gcgagggcgg gatgatcggg gccgtaggcg ctttctgtaa
     8401 tcgtcaagcc acgcttgaag agaggctccg cgtcagaaag ccgctgcttt ccaattacaa
     8461 ccgccaaaga caacagcgta ttcgcaagct ctatggaact tggaccagtc tgtcgctcat
     8521 aaatcgcgac tgctcgacgg agctgttgct ccgtgtgcgc gaactgcttc atggcgaaca
     8581 gcgcttttgc atgattcgtc aatgcgacgg ccagctctcg gtgggacttg ccggcgcttc
     8641 gttctacgat gtggatgacc tggctgtaga gtggctccgc tttttccgga tgctcacgag
     8701 caaaatgaag ggctgcccag gtagaaagac tggcggcagt ttgaggatga ttgtcgccga
     8761 gttccttctg tgcggtttca tacgaccgtt tcaagatggg ctctgcttcg tccaatcgct
     8821 tttgcgtgag gaggagggcg ccgagatggc gaagagcca
//
LOCUS       DAOQEW010000155         8847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2639_length_8847_cov_6.498635, whole genome shotgun sequence.
ACCESSION   DAOQEW010000155 DAOQEW010000000
VERSION     DAOQEW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_8847_cov_6.498635"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLS35_14735"
     CDS             complement(<1..856)
                     /locus_tag="PLS35_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNI19976.1"
                     /translation="MLGRLWGAWRGGSARIDAGETTQRVVPSQIEITLEDLAKDVWLP
                     LNLDFIQQTGELVEEAEPTVSPVEHAAAELEETIAPASLASPSAPALVVGPSSAQLTT
                     PPEEVPQAATPDPATSPRPVSSGRSSALIRDFIMPYQPVIESQRALEPLLTVVKILEE
                     YGDCPSIVTAGVERDEECTDLYSIRDTLAKVTLRDHTYRVTELALAVLKQTYRDGDLM
                     IPKVLVAALGHDLGKIPRFRATAAHAMGDHPVVSALKLQECFAGTSIPWFSEVLDAIK
                     GHHRIGKDR"
     gene            complement(858..1307)
                     /locus_tag="PLS35_14740"
     CDS             complement(858..1307)
                     /locus_tag="PLS35_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19977.1"
                     /translation="MSNREAEQCATGNVSRARWAFDVTPGREKHVVLQLRELGCTSVE
                     RPTKGYVIAQCDPTAVPVIKGLPGVVRALPLVDTDVFEDLSNEPLPAKVSDLVQVSSG
                     PYAQMTGIVRALEGEDVLIDVSLMGRVVRVRAKTAQVSVIPLPEPWR"
     gene            complement(1349..1873)
                     /locus_tag="PLS35_14745"
     CDS             complement(1349..1873)
                     /locus_tag="PLS35_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNI19978.1"
                     /translation="MVSLVVLPDSVGAEMPPDRFWYVNHSCVVAAANRYAVTVQILEA
                     IILVESEGDPHAVNVNRDGKGDRRGPLSLKQAKALVAELWKTGANFDVGIAQINSVHM
                     RHYKIDPVHFLDPCINIQWAAFVLRQKINEYQETWTAVGRYNGSRNIAGYSWKVYRAL
                     ERLAGIRRARGLLR"
     gene            complement(1949..2593)
                     /gene="traN"
                     /locus_tag="PLS35_14750"
     CDS             complement(1949..2593)
                     /gene="traN"
                     /locus_tag="PLS35_14750"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HNI19979.1"
                     /translation="MTTAQSAAETLPPDSITCGSQNSSVPFCVDTNTPPNQNLALAAT
                     WLSMTKNVEEDWDPDRLRIFTGKRSTCDFSTIGRSLINCCDSDPDKLIGKCSDEEIAL
                     ARDKQDWKARLIGTHCVDKGPFGVCLRKEEVYCTMKSELGRMVQEQGRAQLGMTWGSA
                     DAPQCDGFTVDQFSGLNFQAMDFTEWYKNVSANIDPAVITKEMATKICTYTGTC"
     gene            complement(2978..3469)
                     /locus_tag="PLS35_14755"
     CDS             complement(2978..3469)
                     /locus_tag="PLS35_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNI19980.1"
                     /translation="MKILTVDVIPGHAYRYFQALIQIETVGIASWRDAVVRFTDFWGG
                     RAKTYDSPIVHATQSMVDRLAQSAASLGADLIIGFDIQIHQVTARYWGWGMAQIVMRG
                     TPIQFTDRHAMGTIFAGESAPFVASGEPPQHPADGNFQGEAAGMAEMPVPSLDTARFG
                     TRV"
     gene            complement(3476..4453)
                     /locus_tag="PLS35_14760"
     CDS             complement(3476..4453)
                     /locus_tag="PLS35_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19981.1"
                     /translation="MWPRMAVVGLAVLSGCVSAGEYQDLETKYQHQLAVKEVMETEHD
                     ELSHRVDNVSQVYRHVAQEQMGLKAQAETLQGEILTVKGVVKDTQSQYEAQRAQFAQQ
                     TQQLSGVDARLQNIAGKIETLTETTVALANRFERVELSIGKLGKSLQARGGAPEVSKT
                     RVQVGEGKSGGAQKADPVAKAGSPNMEGSAGAGAVGSGSEPGGTSLAAAPSPVAPTTA
                     PAKPDVPMVVSPKVVEAKFEPAGSKPDADKGWLRRFLDGFRGGNGQVAEPVSTPPTAG
                     SVGKASPGRPLPPSVDGEVMAAPMNTLTISTEGDAPKKAEVSPIDTEKR"
     gene            complement(4468..5139)
                     /locus_tag="PLS35_14765"
     CDS             complement(4468..5139)
                     /locus_tag="PLS35_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19982.1"
                     /translation="MAHGVDVEQQPGMVRREGPSLRSASPTNGRRPDRAEENRKRMED
                     PNSILLLPQTKAGMACVSLLYMFDKVVHRLRRAAGSQLSVEQVRNELARVEQWVARAK
                     GLLRELGVGEFELLPQELYPDLKERSVMAARRNARVITPRVGDTVRLVPVIRALDSAL
                     NSIRLRAEDLSPYVKHFQAVDELVVALHDVIGHVARVTVTEYEPRGAVKAFLQEGKES
                     QSTLS"
     gene            complement(5155..5784)
                     /locus_tag="PLS35_14770"
     CDS             complement(5155..5784)
                     /locus_tag="PLS35_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19983.1"
                     /translation="MARVIRMDPGHSAYLLWADSIGRYAILPHQHADRQYKVRDSLMG
                     AVYKVGDEFPMLSQRSMHYFRRIAELVLAPVLADDHVHIKRVATSRAASFVKVAVDTY
                     RSGEDVIAACKPLLYEFRTYTSRTIALVKYHSELESYIREALAPAPSRSVRRVDVYRE
                     EHQARVIVEPGTIAQFLGRKGMNAAVASKLTGYGLVLVDEGDLGKVGGA"
     gene            complement(5905..6171)
                     /locus_tag="PLS35_14775"
     CDS             complement(5905..6171)
                     /locus_tag="PLS35_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19984.1"
                     /translation="MTPTVHPKPSLWSDGATRGGSTAVVEVMSRDSLNVCNVVGVKTG
                     AQDTPVTNPSRITLIVRQDDSDVSATRARTFSLDDSRSGRGLFR"
     gene            6252..6440
                     /locus_tag="PLS35_14780"
     CDS             6252..6440
                     /locus_tag="PLS35_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19985.1"
                     /translation="MALTIRSGAADQETAILEFVDIQKAERIRYDVLHFSRDWTEDAR
                     MFKDYLQTIFHAKYQSCS"
     gene            complement(6875..>8847)
                     /locus_tag="PLS35_14785"
     CDS             complement(6875..>8847)
                     /locus_tag="PLS35_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19986.1"
                     /translation="TLSGTFTALSHGEANQPGVPTAFFGSAIDNSKPGSPAFQANALL
                     YDGQVSFFDGREGGTKSISGERNLTDGTHLSGQFTQLNTMGQDGHQIEMFHGYAQQPG
                     APARDVTGLIDNGNLVAFNGTSGSHARDIVEKGSGVSGRQYGFQDTLMAPGGTHSVST
                     FEGVSVDAQGRAFKNSSVELDGVRVYSDQMRGSRQMTVEFEQSLRGYNDEVQSIDKES
                     GWRRINDGHRDMDVYANLYYGPTSSETGERKLLGGTITNVTDNTYSTFMKDGEGNEVW
                     GIARSLEDPNSEAMSGSFDQTLKVVAAKNGAVDTQVLGPMKSDGGQREVLYHSVLGGL
                     DSQYFNNERKSGVFTVPDPMHPGQTKELQGTFYYSPQSGQLIASEVTDVQTGKVGQYR
                     TDAQGQEHFQVVEYTQSPDGASVIASTRELSKHEYAAHGFAVNDTLDSSGNVLSSSGN
                     KGADFHDFNRVQFHHDSGANLTITQMAFAAKGTDITNPTQTDEAIAYGLEFGRIGMDG
                     ASRYLILKGRMGKFGGGAAGGTPFQSGGGPLPDGPGGGGGPSGLLDSHQNMGAWWKEY
                     SGVMDNAISFARSSVEHLEKTGRAQTMSPEELQNFAANAAYNAVRTSAIGMLGEEASN
                     AFGTMPKWQVGQESLLKVERTQPSAPSTSKEK"
BASE COUNT         1817 a         2904 c         2159 g         1967 t
ORIGIN      
        1 gccgatcctt cccgatccga tgatggccct tgatggcatc caacacttca ctgaaccatg
       61 ggatggacgt cccagcaaag cactcttgga gcttgagagc actcacgacc ggatgatccc
      121 ccatcgcatg ggcggccgtg gcacgaaaac gtggaatctt gcccagatca tgccccagtg
      181 ctgccaccag taccttcgga atcatgagat ccccgtcccg gtaggtttgc ttgagcaccg
      241 ccaacgctaa ctccgtgacc cgataggtat ggtctcgcaa cgtgactttc gcgagcgtat
      301 cgcgaatgga atagaggtcc gtacattcct catcacgttc gacaccggcc gtcacaatcg
      361 atggacaatc cccatattcc tccagaattt tcaccaccgt cagcagtggc tccagtgcgc
      421 gctggctctc aatcaccggt tgatagggca tgatgaaatc ccgaatgagt gcggacgacc
      481 ggccagagga caccgggcgt ggtgaggtgg cgggatcggg tgtggctgcc tgtgggactt
      541 cctcaggagg agtcgtcagc tgggcggagg atgggccaac cacgagagcg ggtgcactgg
      601 gagatgccag ggatgcagga gcgatggtct cctcaagctc agccgcggca tgctccactg
      661 gactgaccgt cggctcagct tcctcgacca actcccctgt ttgctgaatg aagtccaaat
      721 tcagcggcag ccatacgtct ttggccaggt cttccagggt gatctcaatc tggctcggta
      781 ccacccgctg agttgtttct ccagcgtcga ttcttgcaga gccgccgcgc cacgctcccc
      841 acaatctccc gagcatgtca cctccagggt tccggtaacg gaatcacgct cacttgcgcc
      901 gttttagccc tgacacgcac aacccgcccc atcaagctca catcgatcaa tacatcctcg
      961 ccttccaagg ctcgcacgat ccccgtcatc tgcgcatagg gcccagaact gacctgcacc
     1021 agatcggaaa ccttagccgg aagtggctca ttgctgaggt cctcgaagac gtcggtatct
     1081 actagaggca gcgctctcac cactccaggc agccccttga tcacgggaac ggccgtcgga
     1141 tcacactgag cgatcacgta gcctttcgtt ggacgttcca cgctcgtgca ccccagctcc
     1201 cggagctgca agaccacgtg tttttcacgg cctggcgtca catcaaacgc ccaccgtgcg
     1261 cgcgacacat tcccagtggc acactgctct gcttctcggt tgctcacaga ctctcctttc
     1321 gaaagccgta ccggcccagc cagcgtactc atcgcagcaa cccgcgggct cgtctgatgc
     1381 ctgcgagccg ctcaagtgcc cggtacacct tccagctgta gcccgcgatg ttgcgactcc
     1441 cgttgtatcg gcctacggct gtccacgttt cttggtactc gttgatcttc tgccgcaaga
     1501 caaacgccgc ccattggata ttgatgcagg gatcgagaaa atggaccgga tcgattttgt
     1561 agtgccgcat atgcacgctg ttgatctgcg cgattccgac atcaaaattc gcccccgtct
     1621 tccacagttc cgccaccaat gccttggctt gctttaagga tagtggtcct cgtcgatccc
     1681 ccttcccatc ccggttcaca ttcactgcat gcggatcccc ttcgctctcc accagaatga
     1741 tcgcttccaa tatttgtacg gtcaccgcgt agcggttcgc cgccgccacg acgcaactgt
     1801 ggttcacata ccaaaaccgg tccgggggca tctccgcccc gacactgtcc ggcaagacaa
     1861 cgagactcac caccgcaaga cacagccacg ttgaacggag actttgtggt ctggaggatg
     1921 tcaccctgac gcccacgata ctcccgccct aacaagtgcc cgtgtaggta catattttcg
     1981 tcgccatttc cttcgtgatc acggccgggt caatattcgc tgacacattt ttgtaccatt
     2041 cggtgaaatc catcgcctgg aaattcaacc cgctgaactg atctaccgta aatccgtcac
     2101 actggggcgc atccgctgat ccccaggtca tgcccaactg cgcacggcct tgctcttgaa
     2161 ccattcgccc gagctcggat ttcatagtgc agtagacctc ttctttgcgg aggcacacac
     2221 caaacggccc cttatccacg caatgtgttc caatcaaacg ggccttccaa tcctgtttgt
     2281 cacgcgccag cgcgatctcc tcatccgagc atttccctat caatttgtcg gggtccgaat
     2341 cacagcaatt gatcagggag cgcccgatcg tgctgaaatc acaggtactc cttttgcccg
     2401 tgaagatccg cagccggtcc ggatcccaat cctcttcgac attcttcgtc atcgataacc
     2461 aggttgccgc caacgccaga ttctgatttg ggggagtgtt ggtatccacg caaaacggca
     2521 ctgaactatt ctggctgccg catgtgatgg aatccggcgg tagggtctcc gccgcagatt
     2581 gagccgtcgt cactgcaccg ctcgtccgta agtcccatcc atcacggtcc accctgctct
     2641 gccgtatttg ggtgtacctc tcatcgctac cggcggccgt gccgccagcc gcactcaaac
     2701 ttccaggatc atcgtaatac ttctcttcag cggccccgga acatcctgcc gtgacacagc
     2761 ccggcgttcc tgagtaccgc ggtactcttg tcatgttcgg cgccaaaatc cccccaccaa
     2821 tcaagcccga ggacttttgc ccctccacct cgcactttgc tttgaggtag gcatcatcaa
     2881 tcccgctaca atcggcacgg gcctctctcg caccacacgc cagacagacc gcaaggaaaa
     2941 cccccgtgag cgtgcgtttc atcgctaaca gcctcctcta tacccgcgta ccgaaccgag
     3001 ccgtatccaa cgatggcacc ggcatctcgg ccatccctgc ggcttctccc tggaaattcc
     3061 cgtccgccgg atgttgtgga ggctcgccac tcgcgacgaa gggggccgat tcacccgcaa
     3121 agatcgtccc catcgcgtga cgatctgtaa actggatcgg cgttccgcgc atgacgattt
     3181 gcgccatccc ccacccccag taccgtgcgg tcacctgatg gatctgaatg tcgaatccaa
     3241 taatcaggtc ggcccccagg gaggcagcgc tttgcgcaag ccgatccacc atgctctgag
     3301 tcgcatggac gatcgggctg tcgtatgtct tcgcgcgccc gccccagaaa tccgtgaaac
     3361 ggaccacggc atcccgccag gacgcgatcc cgacagtctc gatctgaatc aacgcctgaa
     3421 agtaccgata ggcatgccca gggatgacat ctaccgtgag aattttcatt cctgcctatc
     3481 gcttctcggt atcaatcggt gacacctcag ccttcttcgg ggcatccccc tcggtgctga
     3541 tcgtcagtgt gttcatcggc gcggccatca cctcgccatc gaccgacgga ggaagggggc
     3601 gtccaggtga ggcttttcct actgaaccgg cagtcggtgg agtagacaca ggttccgcga
     3661 cttgcccgtt ccccccacgg aagccatcca gaaaacgccg caaccaccct ttgtccgcat
     3721 cgggcttgga accagccggt tcgaacttgg cctcgaccac cttcggagaa acaaccatcg
     3781 gaacatccgg tttagccggg gccgtggttg gcgccaccgg gctcggagcg gccgccaggc
     3841 tcgtcccacc tggttcacta ccggagccca ctgcccctgc accggcactc ccctccatat
     3901 tcggactgcc cgccttggcg acagggtccg ctttctgagc acctcccgac tttccctcac
     3961 ccacctgaac acgcgttttg gagacctcgg gagcaccccc tcgcgcctgc agggacttcc
     4021 ctagtttccc tatcgacagt tcgacacgtt cgaagcgatt ggctaaggcg accgtcgttt
     4081 cggtgagggt ttcgatcttc cccgcaatat tttgcagacg cgcgtcgaca ccggacagtt
     4141 gttgtgtctg ctgcgcaaac tgcgcccgct gggcctcgta ctgactctgg gtatctttca
     4201 ccacgccctt cacggtcagg atctccccct gcagcgtttc cgcttgagcc ttcaacccca
     4261 tttgctcttg cgcgacatgc cgatacacct gggacacgtt atcgactcgg tgggacagct
     4321 cgtcatgttc ggtctccatg acttccttca ctgcgagttg atgctggtac ttggtttcca
     4381 agtcctgata ttccccggcg ctgacgcaac cggacaacac cgcgagacca acgaccgcca
     4441 tgcgtggcca cataccgtgc tcctttctta cgacagagtt gactgactct ctttcccttc
     4501 ctggagaaat gccttcaccg cgccccgcgg ttcatactcc gtcaccgtca cgcgggcgac
     4561 atgaccgatg acatcgtgca gggccactac gagctcgtcg acggcctgaa aatgtttcac
     4621 atacggactg agatcttccg ctcggagacg aatactgttg agcgcgctat cgagtgcgcg
     4681 aatcaccggc accagtctca ccgtgtctcc cacccgtggt gtaatgaccc gcgcatttcg
     4741 cctcgccgcc atcacgctcc gctctttcaa atcgggatac agctcctggg gaagcaactc
     4801 gaactcgccc actccaagtt ctcgtaggag cccctttgcc cgtgccaccc attgctcgac
     4861 acgagccaac tcattgcgca cttgctcaac ggagagctga gaccctgctg cccgccgaag
     4921 ccgatggaca accttgtcga acatgtacaa cagggacaca caggccatcc ccgcctttgt
     4981 ctgtggtaac aggagaatcg aattcggatc ttccatccgc ttcctgttct cttccgcccg
     5041 atcgggcctt cgtccgttcg tcggagacgc actccgaaga gacgggccct cacgccgtac
     5101 catccccggt tgctgttcta cgtccacccc atgagccatg cctcacctcc tcgattacgc
     5161 ccctcccacc ttacccagat ccccttcgtc cacgagtacc agtccatatc cagtcaattt
     5221 gctagccacc gcagcattca tccccttgcg accaagaaac tgcgcaatcg tgcccggctc
     5281 gacgatcact cgcgcttggt gctcctcccg atacacatcc actcgacgca ccgaccgact
     5341 cggagcaggc gctaacgcct cgcgaatgta agattccaac tccgagtgat acttcacgag
     5401 agcgatcgtg cgggaggtgt aggtacgaaa ctcgtacaac aggggcttgc aggccgcgat
     5461 gacatcttct cccgagcgat aggtgtcgac agccaccttc acaaacgacg cggctcgaga
     5521 cgtcgccacg cgcttgatat gcacatggtc atccgccaag accggagcca gcactaactc
     5581 cgcgatacgg cggaaatagt gcatcgatcg ctgcgagagc atcggaaact cgtcaccgac
     5641 cttatagaca gcccccatca gggaatcgcg gaccttgtac tgacgatcgg catgctgatg
     5701 cggcaggatg gcgtaccgcc cgatggaatc cgcccacagc agatatgcgc tgtgccccgg
     5761 atccatccga atgacccgag ccatcactaa gtctcccacc tgcaccattc gttactcgcc
     5821 tgcgctatag ttttttgacc tgtgtatgca cctgccatgt gcctgtagtt cgagacccgg
     5881 tctcacaact atgcccaaca ggtattaccg gaacagtccc cgtccgcttc ggctatcgtc
     5941 cagagagaag gtccgcgccc tcgtggcact tacatcgctg tcatcttgcc ggacgatcag
     6001 cgtgatcctg ctcggattgg tcaccggggt atcctgtgcc cccgtcttca cgccgaccac
     6061 gttgcagacg ttcaaagaat cgcgcgacat cacctcaacg acggctgtag agccgccacg
     6121 agtggcaccg tcactccaca gcgatggctt gggatggacg gtgggagtca cgcagctcgt
     6181 cggggagacg aaccggatca gacgagaggg ggtttacagc tgttcttttt gggtaatgga
     6241 ttcccgcctc tgtggcactc accatcagga gcggagcggc cgatcaggag acggccattc
     6301 tagagtttgt ggatattcag aaggcggaac gaatcagata tgacgtgttg cactttagcc
     6361 gtgactggac ggaagatgcg cgtatgttta aggactatct ccaaaccatc ttccacgcaa
     6421 aatatcagtc ctgctcttag cagtctcact tcgcatcctg cacctcaagc acgcaatcac
     6481 catacacagt gattaacaat gaccctggat aggtcctata ggtctttttg tctgcctgaa
     6541 gttcagaggt ttccccgaca gtgttcacga tcctaatatt aaccattgct cgcccatttc
     6601 tgcttccccg acactgttct acaacttcat ttaatacagc cagagaactc ccactggagg
     6661 agggactaaa ttgctccgac ccaacagcga tagttgagcg cttgattggt actcctggaa
     6721 tctccgagcc aacgaacgcc cctaaccatc catcttttgg cgactccgct ctcgcaccac
     6781 ctgactgact gacaacgatt gcgaccatca ccgcaactag cgctatctga tacggccgca
     6841 tgtgacctcc actctggaca aaaccattcg ctacctactt ctctttacta gtgcttgggg
     6901 ccgatggctg tgttcgctca accttcaaca acgactcttg gcctacctgc cacttaggca
     6961 tggtcccaaa cgcattcgac gcctcttcac ccaacatgcc gatggccgat gtgcgtaccg
     7021 cattataggc ggcattggca gcgaagttct gtagttcttc gggagacatt gtttgtgcgc
     7081 ggccggtctt ttccaaatgc tccacagaac tacgcgcaaa ggatatagca ttatccatca
     7141 cgccggaata ctctttccac caggcaccca tattttgatg cgagtccaag agaccacttg
     7201 ggcccccacc gccaccaggt ccatccggca ggggtccacc gcctgactga aacggtgtcc
     7261 ccccagccgc tccgccacca aacttcccca ttcgtccctt gaggatcaaa tatcgcgaag
     7321 ccccatccat tccaatccga ccaaattcga ggccataggc aatcgcctcg tccgtttgcg
     7381 tggggttggt aatatcggtg cctttggccg caaacgccat ttgggtaatc gtcaaattcg
     7441 ctccactatc atgatgaaac tgcacgcgat tgaagtcatg aaaatcggct cccttgttac
     7501 cggacgaact cagcacattc ccgctgctgt cgagagtgtc gttgaccgca aatccatggg
     7561 ctgcatactc atgcttcgat agctccctgg tacttgcaat cacgctcgcg ccgtcaggcg
     7621 actgtgtata ctccaccacc tggaagtgtt cttgcccttg cgcatcggtg cggtattgcc
     7681 caacctttcc tgtttgcaca tccgtgactt cggacgcaat caactggcca gattgaggac
     7741 tatagtagaa cgtcccttgt aactctttcg tctgtccggg atgcattgga tcaggcacgg
     7801 tgaagacgcc tgacttgcgc tcattgttaa agtactgcga gtccaatccg cctaacaccg
     7861 agtgatacaa gacctcgcgt tggcctccat cacttttcat cggaccaagg acttgcgtgt
     7921 ctaccgctcc attcttggca gccacgacct tgagtgtctg atcgaaagag ccgctcatag
     7981 cctcgctgtt tggatcttcc aaagacctgg caatccccca cacctcattg ccttcaccgt
     8041 ccttcatgaa ggtggaatag gtgttgtcgg tcacgttggt aatcgtcccg cccagtaact
     8101 tacgctcgcc cgtctcactg cttgttggac cgtagtagag attggcgtat acatccatat
     8161 cgcgatgccc gtcattgatc cggcgccaac ctgactcttt atcgatcgac tgtacttcat
     8221 cgttgtaccc tcgtagggac tgctcgaatt ccacggtcat ctggcgcgat cctctcatct
     8281 gatcgctata cacacggacc ccatccaact caactgacga gtttttgaag gctcgcccct
     8341 gggcgtccac agacacgccc tcaaaggtgc ttacggaatg tgtgccccct ggagccatca
     8401 gggtatcttg gaacccatat tgccgaccag acaccccaga tcctttttcg acaatgtctc
     8461 tggcgtgaga cccagaggtg ccattaaacg ccacaaggtt gccattatca atcagccctg
     8521 ttacatcacg ggccggtgct ccaggttgct gagcatatcc atgaaacatc tcgatttgat
     8581 ggccgtcctg tcccatcgta ttcaattggg tgaactgacc tgacaaatgc gtgccatccg
     8641 tcagattacg ttccccggaa atactcttgg tccctccctc tcgcccatca aagaagctta
     8701 cctggccgtc gtatagcagc gcattggctt gaaacgccgg cgaccctggc ttgctattgt
     8761 cgatcgccga gccgaagaag gctgtcggca ctccaggttg attggcttcc ccatggctca
     8821 atgccgtgaa cgtgccgctc agggtat
//
LOCUS       DAOQEW010000156         8829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2649_length_8829_cov_7.213130, whole genome shotgun sequence.
ACCESSION   DAOQEW010000156 DAOQEW010000000
VERSION     DAOQEW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2649_length_8829_cov_7.213130"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLS35_14790"
     CDS             complement(<1..523)
                     /locus_tag="PLS35_14790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNI19987.1"
                     /translation="MDSPIKVLDVDLAHPLQDLGGLDPYRSVWGLLRLNGRPVGDVTV
                     PIRDGVCTAESMYEAIVLQQGRPILQALIAHHLHDPVSRAGIRTLDPIGMDDMPANVW
                     PLVTVAVCTRDRSEGLHTCLHALQKLDYPSLDRVVIDNAPQSDRTERLVREEFPAVRY
                     VREPRPGLDWARNR"
     gene            complement(733..2058)
                     /locus_tag="PLS35_14795"
     CDS             complement(733..2058)
                     /locus_tag="PLS35_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNI19988.1"
                     /translation="MTAFTVLLLLFLIRPVHAADYYLTATGDDRDTGLSSDHAWRTLD
                     RVNQLELLAGDRILLEAGTTFLGPLLLDGADRGTESAPITVTTFGNGPAVIEAGEGMG
                     IFVRSTAGVILSNLHVSGNHHPHNNSDGIAIHNDLDGALLLTGITIDHVEVSGFGKNG
                     ISIGGWNGDSGFRNVRVTHTRIHDNGLNGLVTYAQHPRANKDVYIAYVEAYRNTGLPG
                     VVPHSGSGIILGNVENGVIEWSKAYENGRLGNAGVGIWTYDSTKVVIQHNESFANRTS
                     GEADGGGFDLDGGVTDSVMQHNFSHDNDGAGYGLYEYAEAPPWYGNTVRHNLSVNDGR
                     KNGAAGIQIWNGGTSLLADATIHDNIVMMGPSEDVTPSAIAFKSPSQGFSIYQNAFIA
                     SGEAILVFKPAEQVHLHMSDNRCWSMTTRSLWDESLEREVCAIYFRFPG"
     gene            complement(2439..2954)
                     /locus_tag="PLS35_14800"
     CDS             complement(2439..2954)
                     /locus_tag="PLS35_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNI19989.1"
                     /translation="MIFLGRRMTVRWSGEGKEQAMALIEKSIEVNVPVRTTYNQWTQF
                     EEFPKFMEGVTQVQQVDSTHLHWKAQIAGKEKEWNAVITEQRPDARIAWKSTDGAKNA
                     GVVTFHRLSDQKSKVMLQMDYEPEGVVENIGEVIGAVSLRVEGDLSRFKEFIERRGHE
                     SGAWRGEVKGK"
     gene            complement(3076..3537)
                     /locus_tag="PLS35_14805"
     CDS             complement(3076..3537)
                     /locus_tag="PLS35_14805"
                     /inference="COORDINATES: protein motif:HMM:NF039411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HNI19990.1"
                     /translation="MERKRKKVVTDADQQIRNYYEHQPPSETEAFPDLSETIPTSEQL
                     MGKVGHMEQDRSGLNGADPDVSPQGIDAGTETPGGSEPTPDQDLVDEIGKAAGVTYQD
                     DEPLNFDEKIAAHDEARWELNPASSEDFEERQSELRGKMPSRRIRNGEENS"
     gene            complement(4077..4289)
                     /locus_tag="PLS35_14810"
     CDS             complement(4077..4289)
                     /locus_tag="PLS35_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19991.1"
                     /translation="MRLRYTLGFLFGVLLLSASCDQGSKPADKSTPPSNAAPTSSNLK
                     ETHPDQPTGLPQSGEGKQVPTKRREA"
     gene            complement(4949..5251)
                     /locus_tag="PLS35_14815"
     CDS             complement(4949..5251)
                     /locus_tag="PLS35_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19992.1"
                     /translation="MHRLICCTCWLTALLLSGCINLSIRLTPEDLSVKPMAEGKDCAY
                     AVFGFGYGTTTVEQAMANATPPIQNVRSVGVNEFYLLGVARLCLIVVGEGAPGTTK"
     gene            complement(5310..5612)
                     /locus_tag="PLS35_14820"
     CDS             complement(5310..5612)
                     /locus_tag="PLS35_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19993.1"
                     /translation="MRTFCTLTVCLAVLLLTGCVSITNRVTPEDLSVKPMAEGKTCMY
                     TLFELGYGTNTVEQAMANATPPIQKIRSVSTSLFFLLFVNKSCLIVVGEGAPASAP"
     gene            complement(5649..5957)
                     /locus_tag="PLS35_14825"
     CDS             complement(5649..5957)
                     /locus_tag="PLS35_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19994.1"
                     /translation="MRTLPRLAAGLSALLLTGCVDLSFRITPENLSVKPMAEGKTCIY
                     TLFNFGYGTNTVEQAMANATPPIQNIRSISVTMFHFLVFESHCLKVVGESAPAGEGAR
                     "
     gene            complement(6442..6684)
                     /locus_tag="PLS35_14830"
     CDS             complement(6442..6684)
                     /locus_tag="PLS35_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI19995.1"
                     /translation="MLKSLMDEFVVNNFETFNSVNTLVDRAVRYVVKRYDAADGRIDK
                     DGFPLDVAQRIGSSTNVRPKPRRRSAIAPLERAASV"
     gene            complement(7601..8707)
                     /locus_tag="PLS35_14835"
     CDS             complement(7601..8707)
                     /locus_tag="PLS35_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNI19996.1"
                     /translation="MYIWELPDWPRFRWQADKLLNPLGDAHLKQGRLLGKMMQLGFEV
                     RLEAELLAVTDEAVKSAEIEGEHLDPSSVRSSVARRLGVRGAALGQEDRRIEGMVAMM
                     LDATGQLDTPLTRERLYGWQASLFPTGYSGLRKVKTGGWRDDADGPMQVLSGPVGREK
                     VHFQAPPAERVDAEMDALLSWLNAPLTIDGILHATIAHLWFATIHPFADGNGRIARAL
                     AERSLARSEQSPQRFYSLAGQIHRERPEYYASLEVTQKGDLDITQRMLWFIECVSHAI
                     DAAEAGCADVLRKAAFWQRHTGLALNARQRKVLNRLLDGFEGKLTARKWAALTKCSLP
                     TAQRDIKELMDQGVLIRNEGGSKNTSYSLTGPPE"
BASE COUNT         1884 a         2575 c         2367 g         2003 t
ORIGIN      
        1 cccgatttct tgcccaatcg agacccggcc tcggctctcg aacgtaccgc actgctggaa
       61 attcctcgcg caccaggcgc tcggttctgt cgctttgtgg ggcattatcg atcacgaccc
      121 ggtcaagtga gggatagtcc agcttctgaa gagcatgcag gcaggtatgc aatccctcag
      181 aacgatcccg cgtacacacc gcgactgtga cgagcggcca gacattcgcg ggcatgtcat
      241 ccatcccgat cgggtcaagg gtgcgaatgc ccgcgcgcga cacggggtca tgcaggtgat
      301 gggcgataag cgcttggaga ataggccgtc cctgttgaag cacgatggct tcgtacattg
      361 attcggcagt gcaaactcca tctcgaatag gcacagtgac gtctccgacc ggtcgaccgt
      421 tcaatcgtag aagtccccac accgacctgt agggatcgag gccgcccagg tcctggagtg
      481 gatgggcaag atcgacatcg agaactttga tgggactgtc cacggtcgat tacgcttcac
      541 tttcttctat acgtctcggc acaagacggt gacttgtcac gccgttgtga tctgaacgga
      601 aacaatcggc agactcgtgc gaacatgcct gccctctcgg ttgaggatgg taggagtaac
      661 gcacgagtca ctcacacact ccggaatgga agacgtctaa ccgtgcagtg accgcaatcc
      721 tggtaaacac cttcatccag ggaaacgaaa gtaaatggca cacacctctc tttcgagaga
      781 ctcgtcccac aaggatcgcg tggtcatcga ccagcaccgg ttatccgaca tgtggagatg
      841 gacttgctcc gccggtttga acaccagaat cgcttcacca ctcgcgataa acgcattctg
      901 atagatcgag aatccctgac tgggagactt aaacgcgatt gcgctcggcg tgacatcttc
      961 ggatggcccc atcatcacaa tattgtcgtg aatggtcgca tcggctaaga gacttgtccc
     1021 gccattccaa atctgaatgc cggcagcacc gttcttgcga ccatcattga cgctgaggtt
     1081 gtgccgtacg gtattgccat accagggagg agcctcggca tattcataca gtccatagcc
     1141 tgcaccatca ttatcgtgag aaaagttatg ctgcatcacg gagtcagtca ctcccccatc
     1201 caaatcgaat ccgccgccat ccgcctcgcc ggaggtacgg ttcgcaaacg attcattgtg
     1261 ttggatcacg actttcgtcg aatcgtacgt ccagattccg acaccggcat tccccaaccg
     1321 cccgttctcg taggcctttg accactcgat caccccattc tccacattgc cgagaatgat
     1381 tccgctgcca ctgtgaggga cgacgcctgg caaccccgtg tttcgatagg cctcgacata
     1441 ggcgatgtag acgtctttat tggcacgagg atgttgcgca taggtaacca ggccattgag
     1501 gccattgtcg tgaatgcgtg tgtgcgtgac ccgtacattc cgaaagccgc tgtcgccgtt
     1561 ccatccgcct attgagattc cattcttgcc aaatccactc acctccacat ggtcgatggt
     1621 gatgccggtc agcaggaggg ctccatcaag atcgttatga atcgcgatgc catctgaatt
     1681 gttatgagga tggtgatttc ccgacacatg caggttcgag agaatgaccc ccgcagtgct
     1741 ccgtacaaag atgcccatgc cttcgccagc ctcgatgacg gcaggcccat taccaaatgt
     1801 tgtgacggtg atcggagcag attctgttcc ccgatctgcc ccatccaaca gcaaagggcc
     1861 gaggaacgta gttcccgctt cgaggaggat gcgatctcct gctaggagct caagctggtt
     1921 gacgcgatcc agcgttctcc aggcatggtc ggaagagaga ccggtgtcgc gatcatcgcc
     1981 tgtagcggtc aggtaatagt ctgcagcatg gacgggacga atgagaaaga gcagcaacaa
     2041 tacagtgaac gcggtcatta ctctgagtag cgggaacgcc tcctcattcc gaggaccata
     2101 cctccctgga tatgagcgag gagactgtcc cacgacacga tcgcttcggg cattgcctgt
     2161 ttgaaccact ctcgctccct gtggcagcca tcagttgttg tgcaagccat tctgtcgcaa
     2221 aggcaccaga cacctcggcc aatagattgc ctccaccgga ctcgcgaaat cgccggtaat
     2281 ctaggcctga gaacagcacg gagtgagctt gcatctgcgg atcctgagcc ggaaaaccgg
     2341 cagtcaggaa gcgcgtctta ggtaggagca caagaatact tgtagcagcg tcttcccatt
     2401 acatgtatgg tctgatccac ttgcgcaatg cccgggcatt acttcccctt gacctcacct
     2461 ctccaggcgc cgctttcgtg cccccgcctt tcaatgaatt ccttaaagcg acttaaatct
     2521 ccttcgacgc ggagcgacac ggccccgata acctcaccga tgttctcgac gaccccttcc
     2581 ggctcatagt ccatctgcag cataaccttg cttttctgat cagacaggcg gtggaacgtc
     2641 acgacgccgg catttttcgc gccgtccgta cttttccagg cgatccgtgc gtctggacgt
     2701 tgctccgtaa tgaccgcatt ccattctttc tctttgccgg caatctgtgc cttccagtgc
     2761 agatgcgtgc tatcaacttg ttgaacctgc gtgacccctt ccataaactt cgggaattcc
     2821 tcaaactgcg tccattgatt gtatgtcgtt cgaaccggga cattgacctc gatggatttt
     2881 tcgatgaggg ccatagcttg ttcctttccc tcgcccgacc atcgcactgt catgcgacga
     2941 ccgagaaaga tcatctcgac tcctcatgac gtcatttagg cattttacct cgttggttat
     3001 aactagggac ttccctagga cctcgttttt ggttctcgcg aaggcgaaag cattgtccta
     3061 ccagctctct cctgttcagg aattttcctc gccgtttcgt atgcgacgag agggcatctt
     3121 tccgcgcagt tccgattgcc gctcctcaaa atcttccgaa gaggcgggat tcagttccca
     3181 acgcgcttca tcatgagccg caatcttctc gtcgaagttc agcggctcgt catcttgata
     3241 cgtcacccca gcggccttgc cgatttcgtc cacaaggtct tggtcaggcg tcggttcaga
     3301 acctcccggc gtttccgtcc cggcgtcgat gccttgcggt gacacatcgg gatctgcgcc
     3361 gttcagtccg gaacgatcct gttccatatg ccccaccttt cccatcaatt gctcagacgt
     3421 cgggatcgtt tcggacagat caggaaacgc ttccgtttcg gacggcggct gatgctcata
     3481 ataattgcgg atctgctggt cagcgtcggt gacgaccttc ttacgttttc gttccatagg
     3541 tgcctcacat ctctgtttgt gcggagataa tcatccccat gaacccaggg gcaacatcga
     3601 ggatctgccg tacgcccccc catcacgccg tctcattccc tgcgcactgg gtcgagacca
     3661 caatcgcacc tcgacactct cccgtccgca ctcaggtgaa atcggtgacc ctgggtgcac
     3721 tcataacgga gacccgacat cccagcctgt atcgctatgg cccgctcgcg ttcgggaaca
     3781 gtctcgcggt gacactcctg ctcgcccaat cgttcgcgac agcggatggt gcgcatgagc
     3841 actccctcat ttgcttcttt ccacgccgat cagaaacacc tgcctttaat gatgtgcttg
     3901 gcatccggag tgatgacaag ggcaggaagc ggcagcctca ggtttggtcg cagcagtttg
     3961 acagcgatca ctgcaatact ttttaccttc gactttgcac gagcaggatg gatgggcaca
     4021 ttttgtagga gccatatcgt cctcctagag gtaagaaccc gtgtatgatc accccttcag
     4081 gcttcacggc gctttgtcgg gacttgcttc ccttcgccac tttgcggcag tccggtcggc
     4141 tggtccggat gcgtttcttt taaattggat gatgtaggcg ccgcattcga gggcggcgtg
     4201 gacttgtcgg ccggcttgga tccctggtcg caggaggccg acaacagtag aacgccgaac
     4261 aagaaaccaa gtgtgtatcg gagtcgcatg ttgtttcctc cgctagaggg tcaggcaccc
     4321 ttgcttcact cgctttcatc ctacgctggt ctctggagga ggctactgga caaagccctg
     4381 gtgccaagtt tcgcagacgg attaaggcaa tcgaaccatg ccttcctcaa gctgagaata
     4441 tctatacgcg ccaagttcag aaggcgtgac agggtgctgc gggaaccaca cttccttaat
     4501 ccttcgcata catgcgtatc gcgagcggtc aaggtcggag acgaactaga gaaagatgtc
     4561 cgatagctga ggcagtagtt gacggcgatg gcaccgcgtt acgccgtcag ttggcggaaa
     4621 atttgccggg aaaggttctg attggagtgg acggcttaga ggtgttgcga tcgattagca
     4681 caaattaagt actaccgcgt ggactgatcc cctagtttga gacagccaac atgggcacac
     4741 ttgcaccagg gcccccagat gattttgagc gtggcctcgc gtccggaccg ccgcctggtg
     4801 ccgcgcgcat tccgtttttt aggcctgcac aggaatcaac accgggagaa gtctcgtaga
     4861 ggaaggacgg caatcactcc aacgtgtgct aatgccaacc cgtctcagtg aaggggttca
     4921 ccccagtcgt cgcggggtca cggtgcgctt atttggtggt gcccggggca ccttcgccga
     4981 ccacgatcag gcagagcctg gctacaccta gcaagtagaa ttcgtttacg cccacacttc
     5041 ggacgttctg aatcggcggg gtggcgttgg ccatggcctg ctcaacggtc gtggtgccgt
     5101 agccaaaccc aaacacggca tacgcgcagt ccttgccctc cgccatcggt ttcaccgaga
     5161 gatcctccgg ggtcaggcga attgagagat ttatgcagcc ggagaggagt agggccgtca
     5221 gccagcaggt gcagcagatc agtcgatgca tcgtatagtc cctccagtac tcggtaatcg
     5281 tcgacagacc gtctccgttg gcgtcgccgc tatggtgcgg aggctggtgc gccctcgccg
     5341 accacgatga ggcagctctt attcacaaac agcaagaaga agagacttgt gctcacgctt
     5401 cggatttttt gaatcggcgg cgtggcgttg gccatggcct gctcgacggt attggtgccg
     5461 tagcccaact caaacagggt gtacatgcag gtcttgcctt ccgccatcgg tttcaccgag
     5521 agatcctctg gggtgacgcg attggtgatg ctgacgcagc cagtgagcag aagcacggcg
     5581 agacagacgg tcagcgtaca gaacgtgcgc atgcggggtc ctcctcttgc gaacacatca
     5641 tgttccaatt agcgtgctcc ctcgccggcc ggagcgctct caccgacgac cttcaggcag
     5701 tgtgattcga agactaggaa atgaaacatc gttacactga tactgcgaat gttctgaatc
     5761 ggcggggtgg cgttggccat ggcctgctcg acggtattgg tgccgtagcc aaaattaaac
     5821 agggtgtata tgcaggtctt gccctccgcc atcggtttca ccgaaagatt ctccggcgtg
     5881 atgcgaaagg agaggtctac gcagccggtg aggagtagtg cggaaagacc ggccgctagc
     5941 ctgggtagtg tgcgcatgtg atgtcctcca agactacggt gtgaggtatg gagctgggtt
     6001 cgtcgctcga caaaatgaac aggcacgcgc ctcaggcgca cggatcgtcg acgacggtga
     6061 taacgtgagc gctacataat tggaaaataa acagatcagt gctgatgctc ggaatatatg
     6121 ccgacatagc gtctcatttc tcgaccagcc tccagccgtt atgctcaaga cacgcttggt
     6181 ctctgatccc cccgtccggc gtagtcggcc agctgaccga tggggcggtt gcctcctgca
     6241 tggccgacgt atcactcggc acgctcggca atcagcctca tgcaaaatcg acactgcgtg
     6301 ccgccaacag tactgcacaa ctcgcacgct cgggagaacg cttttaaagt ttcggcggtg
     6361 aaccacttca cgttcatgtc gtctgccagc cattcccgta ccagtctggg caggtcgctt
     6421 tggtagcgtt gcgttcaatg cctagaccga tgccgcgcgc tcaagggggg ctatggcgct
     6481 tcttctcctc ggcttggggc gcacgttcgt agaagagccg atcctctggg ctacgtcgag
     6541 cgggaaccca tccttatcaa ttcgaccgtc tgccgcgtca tagcgtttca ccacataccg
     6601 caccgcacga tccaccagcg tgttcacgga attgaatgtc tcgaaattgt tcaccacaaa
     6661 ctcatccatg agcgatttga gcaaggctgt gccagatatt tttatctacg catctcgctt
     6721 ttctttgtct cttctcacca tgagtataat tgcttggttg tgaagcggcg ccattttggg
     6781 acttccatct aaaaatatca actggcattt atatggacgc gggctttcaa atcagtcctt
     6841 gtccgcgccg cggcccagtt ttccagtgac gtgcacccag gtcaactcat cgaagttcag
     6901 gtcggacgga tagtggcacc acgcgcagct ctagcagacc ggcctgcgcg gcaacaaacc
     6961 gctgaacccg cttgactgtt cgcgcaggat ggccatctag aatgaggatg atcttgcgga
     7021 tcatgccctg gaggagatgg cacaggaact cgcaaagact gaggcgatca cccggagctc
     7081 gtgcgcaatc gttcgtaccc gatcactgat tccacgaagc ttgtcagcga cgtttttgag
     7141 gatcgtcacc tcagggcttt tcttctcaac aacaccaatt cccttcggcc ctcaaaatca
     7201 cacttcccca ggagtagact tcacacggcg aaccgtcacg gtgtttaaat ccaagaacgg
     7261 ggaatggcgg acgattccgc tcaaccaaac cgtagtggat ctgctctctc ataaatacga
     7321 gcagggtcgt gggctacggg gaattgaaac ccgggttgtg ttctgcagcg aagccggtac
     7381 cgagttggac ggcagcaacc ttcgacgagg atttaccgca gccctgaagg gaggatggtc
     7441 agctttacag gagcgcaggc actcttgtcc gatgatgcca atgttggctg tctcaagcta
     7501 gggggtcagt ccatagccac gccatttcgt tttccacaca cgtacacgct tgcgttgttc
     7561 tggcactttc tcaatcagcg tgagatccac ctcgtcggcc ttattctgga ggtcccgtga
     7621 ggctataact tgtgttctta ctgcccccct cattacggat cagcacccct tgatccatca
     7681 gctccttgat atcacgttga gcagttggca gagaacattt tgtgagagcc gcccattttc
     7741 tcgccgtcag cttgccctca aatccgtcga gcaatcgatt cagcactttc cgctggcgtg
     7801 cattcagagc gagaccggtg tggcgttgcc agaacgcggc cttgcgcaac acatccgcgc
     7861 atcccgcctc ggcggcatcg atcgcatggg acacgcattc aataaaccag agcatccgct
     7921 gcgtgatgtc gaggtcgcct ttctgagtca cttcgagcga ggcgtaatac tcggggcgtt
     7981 cgcggtgaat ttggcccgcc agactataga atcgctgtgg cgactgttcg gagcgtgcaa
     8041 ggctccgctc cgcaagtgcg cgcgcaatcc tcccgttccc atcggcgaac ggatggatgg
     8101 tggcaaacca caggtgtgcg atcgtcgcat ggaggatgcc gtcgatggtg aggggggcat
     8161 tcagccatga gagcaacgcg tccatttcgg catcgacgcg ttcagccgga ggcgcttgaa
     8221 agtgaacctt ctcacgtccg accggacctg agagtacttg catcgggcca tcggcatcat
     8281 cccgccaccc gccggtcttg accttgcgta aacctgaata cccagtcgga aacagcgagg
     8341 cctgccaccc gtacaatcgc tcacgtgtga gcggggtatc cagttggccc gtagcatcga
     8401 gcatcatggc aaccatgccc tcaatgcgtc gatcttcctg accgagggcg gccccacgaa
     8461 cgccaagacg gcgtgccacc gatgagcgga cactcgacgg gtccaaatgt tcgccctcta
     8521 tttctgcact cttgacggcc tcatccgtca cagccagcag ttcagcttca aggcgtacct
     8581 cgaagcccaa ttgcatcatt ttgccaagaa gccgaccttg cttcaggtgg gcatccccta
     8641 gtggattcag taacttgtca gcttgccagc gaaagcgtgg ccagtcaggc aattcccaga
     8701 tatacacatt cgcctcacag tatgatgcaa atatacacct attcgcatca cgaagcttgt
     8761 ggcaatgtaa gtgcttgata aatggagccg gcgagtggaa tcgaaccacc gacctgcggt
     8821 ttacgaaac
//
LOCUS       DAOQEW010000157         8820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2653_length_8820_cov_7.679407, whole genome shotgun sequence.
ACCESSION   DAOQEW010000157 DAOQEW010000000
VERSION     DAOQEW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_8820_cov_7.679407"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLS35_14840"
     CDS             <1..1242
                     /locus_tag="PLS35_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HNI19997.1"
                     /translation="ATLDMEGRMTDRASWRTSGQIRFDEGTIKVENLDEPIRDAFVTL
                     RFDRDRIHIPRMAFHVGASDLRISGSIAQWADQPKARLVVESSQIDLAAFEMSRRRPS
                     ASARQRSSDHLWTDTTLHAFLFADHVYYKKFLLTDLSTKITWDHGLLTVERISGDTNE
                     GQLAGQVKIRTKNGQMEQARSTFRASGIPVERVLSPFQERPALSGWLTTSGKVQAEFE
                     RGVLLPAALTSRQPIQILVEEGRMHHIPVLSTLLSVLNLPAVLQGQVNLEKEGMPFDR
                     LKLAGFINNGVVHTKEFLLDSPVLKISGTGRYDLLADEFDMVLATSPLGSYSAILKRI
                     PLFGHLLSGDRQGFDTAVFELKGSANKPQLRYLPAESLMTGVKGTAQLAFDILVNAIT
                     LPQKAFSMVEEGVTGGEEEDF"
     gene            complement(1266..1637)
                     /locus_tag="PLS35_14845"
     CDS             complement(1266..1637)
                     /locus_tag="PLS35_14845"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNI19998.1"
                     /translation="MPRSGTIFMETRYLRQPMRLVHRSFLFLPLLLCLSAGCLWTGTS
                     PLPDGPKDAVISTNVEAKFAEDQQGGLSGIIVTTEEGIVTLTGTVHKAERKARAAELA
                     RQVKGVRRVKNDLDIRADFAP"
     gene            1739..3019
                     /locus_tag="PLS35_14850"
     CDS             1739..3019
                     /locus_tag="PLS35_14850"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNI19999.1"
                     /translation="MAEPVADICVIGAGAAGLAAGIFAAERQPALRVEVLDGAASVGA
                     KILVSGGGRCNVTHDVVTPEDFFGTRHLVRNVLAAFPVKDTVAWFASLGVELKREETG
                     KLFPVTDKARTVLDALLRRSRELGVVLRPGHRVVNIERRTESEEGGTENLSRFVIRHQ
                     HGETPACRIILATGGRSLPRTGSDGGGYELVRHLGHHVTPTVPALVALMLDERCFHAE
                     LSGLSQEVEIQTLVEGRSVDRRRGSLLWTHFGISGPVVMDASRFWCLARSRGETAEVM
                     GNFLPGRNAEQAREWFLAQAAQNPRRSLLKVLAESLPERYAEVLCRHAGSDPQQACAQ
                     VPRAIRDRLLEALTRFRFPVVRDRGWNFAEVTAGGIPLEEVNFRTMESKLVPGLYLVG
                     EVLDCDGRIGGFNFQWAWATGRVAGRAAAASLGS"
     gene            complement(3044..4069)
                     /locus_tag="PLS35_14855"
     CDS             complement(3044..4069)
                     /locus_tag="PLS35_14855"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNI20000.1"
                     /translation="MHHRHVFSLLLWQRALLLLSLWLASPAAADEIYLLNGDRLTGTI
                     VKMEENLLTIQTDYGGEIKVDWKKVQRLTATSPIKILVPGESRDVLRDFVYGTQGQRE
                     VSEAGQDTSTPLSDITAINLEPFRVAGTVSVGGNNTSGNSSTKAFNSAARLTLYAHRQ
                     RLLVEGKYNYGQANDQVTARNSLASLKHNYFISKQIFIETFGMLEKDTLQALQLRSTI
                     GSGLGYQFYETPTTTLSLSAGIAHVNEHFTNSPNTQTPSGRWSLRWEHSLWPDRVKVF
                     HRHEGFWDINAGNAFRFNADQGLRITVYKNLFFNVEYDLRLNTQPAPGRKKTDEAMIF
                     GVGYEFR"
     gene            complement(4218..5411)
                     /locus_tag="PLS35_14860"
     CDS             complement(4218..5411)
                     /locus_tag="PLS35_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20001.1"
                     /translation="MTIPSLLLRGLIGLTVLMNLAGCLSPPTLTRAVVSYDNAITDSI
                     SKQLLLNIARAHHHQPVHFTGVSNVAATFDFRINAGATPTLTGEAGKTLLPVFGGSVA
                     ENPTISIAPIDGEEFTRRLLTPFQETKFTLLLRQGGDIDLLLRLMAKELRVDDQGEDI
                     AYRNSPTDKAGYEMFRKVVLHLSAIQDANRLYAESMLFERTWTIPAESVTAEGFKALE
                     QDYLVAYDPQQKTYRLRKPVSGRILITNYDPNTLPQEERVRLHEEAERSPMNDVSFDI
                     RPGHYGGEWPLKGEFRLRSFNTMLNFLAQSVEEEPEYAVEKDNRTPPFMDNPVKTMDL
                     LVQESSPSESDLTVQSHGKYYSVNITGPLARWNREAFKLLYQLFQMTVTEVSRSGVPS
                     ITIAK"
     gene            5592..6362
                     /locus_tag="PLS35_14865"
     CDS             5592..6362
                     /locus_tag="PLS35_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20002.1"
                     /translation="MRHSVWVGVMTLLCFTGCGSSAKLISGLSIEELKPVPGILSSAR
                     LLKGTIRSITGDRVEVDTGERAPRYLSLEAGGDEARGLQVGGAVEIMVNDHDDIVAYQ
                     RPAETPPTKVFRGSLGKVFNPQKERVTIRLDTGRDVGFYARSVAMEKLAALEVGETAD
                     FAVDRAYLLVDVLSLGKPRDLGVGSSLPAPQRRVEGAFVTIGDGRMRLRMKIDDIQSF
                     PVRPLLQPAIEQLSPNETVSIYLDGQGQVIDMVRTARR"
     gene            complement(6369..7253)
                     /locus_tag="PLS35_14870"
     CDS             complement(6369..7253)
                     /locus_tag="PLS35_14870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI20003.1"
                     /translation="MLNWFSTSSPSDSVVITGASSGIGAACALALDTLGYRVFACVRH
                     PADGERLQRQAGPRLMPIRLDVTDSASIAAASHTVAAMVGDRGLAGLVNNAGIGVAGP
                     IELLPLADWRRQFEVNVFGLIAVTQTFLPLIRTGHGRIINMGSIAGRASMPFMAPYAA
                     SKHALEAITDALRLELQPWGIRVALITPGAIATPIWGKTRKEVDTWDATWSPDLKDMY
                     QEGFTRIKEAATAAGEQAQPAKVVAEAVAHALRSRWPHTRYLVGSDATIRSWLALLLP
                     DRLNDWIITRIVKLPRRR"
     gene            7396..8796
                     /locus_tag="PLS35_14875"
     CDS             7396..8796
                     /locus_tag="PLS35_14875"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNI20004.1"
                     /translation="MHTEKCKWALLLMMGLSLAAYVSPLNAGTDQKGVVATEKLVTVQ
                     PREIDDVLDNPGMGFADFHFGFGHPPKPDEYPHTTVAYFRWPWAELEPAEGQYNFALV
                     DGVIAQAKAKGETLAIRIVSEYKTGTPQWLLDKGVASIKETDGIFPDYNNPIFLDYHE
                     RLIRAFGERYGRSLDIDHVDIGSVGCWGEWNTACCVGAETQCKAYYPTEANQLAITDW
                     YLKYFSGTPLVMLHGGQLQYAASRGAGWRADCFGDYGYFSPDWNHMEHAYAPVLEAQV
                     VADAWKQGPVQMEVCGYIHEWYERGFDLDRILEKGLEWHVSVLNAKSKPVPAAWRPRF
                     NEFLKKIGYRFVLRELTHPASIGAGESLIIHSRWDNKGVAPIYHAWPLAYRLRSGTDE
                     VVAQWTSAADLKRWLPGPSHLAEDAVVVPSTVPAGVYTLDVGILSEDAKSATVELAIE
                     GKRTDRWYAVSRVEIR"
BASE COUNT         1743 a         2379 c         2824 g         1874 t
ORIGIN      
        1 gccaccctcg atatggaagg ccggatgaca gaccgtgcgt cctggcgcac ctcaggccag
       61 atcagattcg atgaaggcac gatcaaggtg gagaacctcg atgaaccgat tcgcgacgcg
      121 ttcgtgacgc tgcgtttcga tcgagaccgg attcatattc cccgcatggc gtttcatgtc
      181 ggcgcaagtg atctccggat atccggctcg atcgcgcaat gggccgacca gccgaaggcc
      241 cgcctcgtgg tggagtcgtc tcaaattgat ctcgctgctt ttgaaatgtc tcggcgccgc
      301 ccatcggcgt cggcgcgcca gcgatcgtca gaccatctct ggacggatac aaccctccac
      361 gcgtttctat ttgcagacca tgtctattac aagaagttcc tgctcaccga cctctcgacg
      421 aaaattacgt gggatcacgg actgctgacg gtcgaacgga tcagcgggga tacgaacgag
      481 ggccagctcg ctgggcaggt caagatccgc acgaagaacg ggcagatgga acaggctcgc
      541 agcaccttcc gcgccagcgg cattccggtt gagcgagtcc tgtcgccgtt ccaagagagg
      601 cccgcgctct caggatggct gacgacgtcg ggaaaagttc aagcggaatt tgagcgggga
      661 gtgcttctgc ctgccgcgtt gaccagtcgt cagccgatcc aaatcctcgt tgaggaaggg
      721 cgcatgcatc acatcccggt gctgtctact ctgctgtctg tgttaaacct cccggctgta
      781 ctgcaggggc aggtcaacct cgagaaagag ggcatgccgt tcgatcggct caagctggca
      841 gggttcatca acaacggcgt ggtccacacg aaggaatttc tactcgacag tcccgtgctc
      901 aagattagtg gaaccggtcg ctatgacctc ctggcggatg aattcgacat ggtgctggcg
      961 acgagtccgc tcggatccta ctcggcgatc ttgaagcgca ttcctctgtt cggacacctc
     1021 ctgtcagggg atcgccaggg gtttgatact gctgtgttcg agttgaaggg atcggcaaac
     1081 aagccgcagt tgcggtatct tcccgctgaa tcgctgatga cgggtgtgaa gggaacggct
     1141 cagttggcct tcgatatttt ggtgaatgcg attaccttgc cgcaaaaagc cttctcgatg
     1201 gtggaagaag gggtgaccgg aggggaggag gaggatttct gacgcccggc tcaggcaacg
     1261 atgtgttacg gtgcaaagtc ggcccgaatg tccagatcgt tcttgacgcg ccggacccct
     1321 ttcacctgcc gcgccagttc agccgctctc gccttccgtt ctgccttgtg caccgtaccg
     1381 gtcagggtca cgatgccctc ctcggtcgtc acgatgatcc cggagagtcc cccctgctga
     1441 tcctcggcaa acttcgcttc gacattcgtg gaaatgaccg catctttggg accatccggc
     1501 agcggcgagg tgccggtcca caggcatcct gccgaaaggc acagcagcag cgggagaaac
     1561 aggaacgacc ggtggactag gcgcatgggc tgtcgcaggt atcgcgtctc cataaaaatc
     1621 gtcccggatc ttggcatagt gcggctccgg tttccatgcc gagttttggg attgcgcgtc
     1681 ctgttcgcaa cgggcatcat gcggcgccag gaatcagtat gttggagcac gagggcagat
     1741 ggcagagccg gtggcggata tttgtgtaat cggggcgggc gcggctggat tggcggccgg
     1801 gatttttgcg gctgaacgtc aaccggctct cagagtggaa gtgttggatg gggccgcgag
     1861 cgtcggcgca aaaattcttg tttcaggcgg ggggcgctgc aacgtcacgc atgatgtcgt
     1921 cacaccggaa gattttttcg gtacccggca cctcgtccgg aatgtgcttg cggcatttcc
     1981 agtgaaagat accgtcgcgt ggtttgcctc gctgggtgtc gaactcaagc gtgaagagac
     2041 cgggaagttg tttccggtga ccgataaggc cagaacggtc ctcgacgcgc tcctgcggcg
     2101 aagtcgcgaa ctcggtgtgg tccttcggcc ggggcatcgc gtcgtcaaca ttgagcgcag
     2161 gaccgaatcg gaagagggcg gcacagaaaa tctatcccgg tttgtgattc gtcatcaaca
     2221 tggagagaca cctgcgtgtc gcatcatcct ggccaccggc gggcggtcgt tgccgcgcac
     2281 cggaagcgac ggagggggat acgaactggt acggcatctg gggcatcacg tcaccccgac
     2341 cgtaccggca ttggtggcgt tgatgctgga tgagcgctgt tttcatgcgg agctttcagg
     2401 cttgtcacag gaggtggaga ttcagacgct ggtggaaggg cgatcggtcg accggcgtag
     2461 aggcagtcta ctctggacgc atttcggcat cagcgggccg gtcgtgatgg acgccagtcg
     2521 tttctggtgc ctggcccgct cgcgaggtga aacggccgag gtcatgggta actttctgcc
     2581 cggccggaat gcggagcagg cgagagaatg gttcctcgcg caggcggccc agaatcctcg
     2641 ccggtcattg ctgaaagtgc ttgcggagtc attgccggag cgatatgccg aggtgctttg
     2701 ccgtcacgcc gggagcgatc cgcaacaggc ctgcgctcag gtgccacggg ctattcggga
     2761 ccggctgctg gaggccctca cccgctttcg ttttcctgtc gtgcgtgatc gcggctggaa
     2821 cttcgctgaa gtgacagccg gcgggatccc cttggaagag gtcaatttcc gcaccatgga
     2881 atcgaagctc gtgcccgggt tgtatctggt gggagaagtg ctcgactgtg atggacggat
     2941 cggcggattt aattttcaat gggcctgggc gaccggccgg gtggcgggcc gcgcggccgc
     3001 ggcgagtctc ggttcgtgaa ccggcggctt gatggggaag tgcttagcgg aattcatacc
     3061 cgacgccgaa gatcatcgct tcgtcggtct ttttccgtcc cggcgccggt tgtgtgttca
     3121 gccgcagatc atactcgaca ttgaaaaaca aattcttgta gaccgtgatc cgaaggccct
     3181 gatcggcatt gaaccggaag gcgttgccgg cattgatgtc ccaaaatcct tcatgccgat
     3241 ggaacacctt gacgcgatcc ggccacaggc tgtgctccca acgcaggctc cagcgtccgg
     3301 aaggggtctg cgtgttcgga ctattcgtga aatgttcatt cacatgggcg ataccggccg
     3361 ataacgaaag cgtggtggtc ggggtttcat agaactgata acccaagccg ctaccgatcg
     3421 tggaccgaag ttgcagcgcc tgcagcgtgt ccttttccag catgccgaac gtttcgatga
     3481 agatctgctt gctgataaaa taattgtgtt tcaagctcgc gagggagttt ctcgcggtca
     3541 cttgatcgtt cgcttgccca tagttgtatt tgccttccac cagcagccgc tgccgatgtg
     3601 cgtagagtgt cagtcgggcg gcgctgttga aggccttggt actgctgttg ccggacgtat
     3661 tgttccctcc caccgagacg gtgcctgcta cccggaaggg ctcaaggtta atagcggtga
     3721 tatccgacag cggcgtggac gtatcctggc ctgcttcaga gacttcgcgt tggccctgcg
     3781 tgccgtagac gaaatcgcga aggacatctc gcgattcccc gggaaccaga attttgatcg
     3841 ggcttgtcgc tgtgaggcgc tggacctttt tccaatcgac cttaatttcg ccgccatagt
     3901 ccgtttgaat cgtcaggaga ttttcttcca tcttgacgat cgtgccggtg agtcggtcgc
     3961 cgttgagcaa ataaatttca tcagcagcag ccggtgaggc aagccacaaa ctcaaaagaa
     4021 gcaacgcccg ctgccagagc aggagactga agacatggcg atggtgcatg gatgtaaacc
     4081 ggaactcaga cgggcggcgg ctcgaagtcg aggcgaggtg ctgccatctg tagaccaggt
     4141 ctcaccctaa tcgagaggag aagaagtttc aagtgcacgt gcgagaaagt gatctggcgt
     4201 caaccagcgg gcggagatta tttcgcgatg gtgatgctgg gcacgccgct tcgtgacact
     4261 tccgtgacgg tcatctggaa cagttggtag agcagcttga aggcttcacg gttccatcga
     4321 gccagtgggc cggtgatgtt gacggagtaa tacttgccat gggactgcac ggtgagatcc
     4381 gattcggacg ggctggactc ttgtaccagg aggtccatgg ttttgactgg attgtccata
     4441 aaaggcggcg tgcggttatc tttctcgacc gcgtattccg gttcttcttc cacggattgc
     4501 gccagaaaat tgagcatggt gttgaagctg cgcagacgga attcgccctt cagcggccac
     4561 tctccgccat aatgtcccgg acgaatgtcg aacgacacat cgttcatggg gcttcgttca
     4621 gcttcctcgt gcagccggac ccgttcctct tgcggaagtg tgttgggatc gtagttcgtg
     4681 atgagaattc tgccggacac cggtttacgc agccggtagg ttttttgctg cggatcgtag
     4741 gcaaccagat agtcttgctc cagtgctttg aacccctctg ccgtcacgga ttcggcggga
     4801 atagtccacg tgcgttcgaa cagcatcgat tctgcataga ggcgattggc atcctgaatg
     4861 gcggagaggt gcagcaccac ttttcggaac atctcatagc ccgccttatc ggtcgggcta
     4921 tttcgatagg cgatatcctc tccctgatca tccacccgca gctcttttgc catcagacga
     4981 agcaggagat cgatatcgcc gccttgtcgg agcaggagcg tgaacttggt ttcttgaaag
     5041 ggtgtcagca ggcggcgggt gaattcttcc ccgtcgatcg gtgcaatgct gatagtggga
     5101 ttctccgcca cactgccgcc gaacaccggc agcagcgttt tgccggcttc tccggtcaaa
     5161 gtaggagtcg ctccggcgtt gatccggaaa tcgaaggtgg ccgcgacgtt tgagacgcct
     5221 gtaaagtgaa cgggctgatg gtgatgggcg cgcgcgatat ttaacagcag ctgtttcgaa
     5281 atggagtcgg tgatggcgtt atcgtaggag acgacggcgc gcgtgagcgt cggcggcgag
     5341 agacagccag cgaggttcat gaggacggta agaccgatca ggccacgcag caaaagagat
     5401 ggaatggtca taggcatcga gttcggtgat gaaggtggcc catcaacagc ggttctagca
     5461 tacctattcc aggaagccta gggggatgga tgggcatgtg tttcgccgga cggcgcgagc
     5521 tcgcctcggg gcggcgattt ggtttatcat cgggcactgt gatgaccagg aacagaatga
     5581 gagctcgaca catgaggcat tccgtgtggg ttggggtgat gacgctgctg tgcttcaccg
     5641 gatgcggcag ttcggcaaag ctcatttccg ggctgagcat cgaagaattg aaacccgtgc
     5701 cgggtattct gtcgagtgcc aggcttttaa aagggacgat ccgcagtatc accggcgacc
     5761 gtgtggaagt ggacaccggc gaacgggccc cccgttattt gtcgctggag gcgggcggtg
     5821 atgaagcccg cgggttgcag gtcggcgggg cggtggagat tatggtcaat gaccatgacg
     5881 acatcgtcgc gtaccagcgg ccggccgaga cgccgccgac caaagtgttt cgcggatctc
     5941 tgggcaaggt gtttaatccg caaaaagaac gcgtcacgat tcgtctcgat accgggcgcg
     6001 atgtggggtt ctacgcgcgg tccgtcgcga tggagaaact cgcggcgctg gaggtgggag
     6061 aaacggccga ttttgcggtg gatcgcgcct atctgctcgt ggatgtcttg tcgctgggga
     6121 agcctcgcga cctgggagtc gggtcttcgc tgcctgcgcc acaacgtcgc gtggaagggg
     6181 cgtttgttac gatcggcgac ggccgcatgc ggctgaggat gaagatcgac gacattcaat
     6241 cctttcccgt gcgtcccttg ttgcagccgg ccatcgagca actgagcccg aatgagaccg
     6301 tctcgattta cctcgacggg caggggcagg tgatcgatat ggtacggacg gcccgccggt
     6361 aagctttgtc agcggcgccg gggaagcttc acgatacggg taatgatcca atcgttgagg
     6421 cgatcgggca ggaggagtgc cagccatgag cgaatcgtgg catctgagcc caccaggtat
     6481 cgagtatgtg gccagcggga ccgcagcgca tgcgccaccg cctcagccac caccttcgcc
     6541 ggctgagcct gttcccctgc tgctgtggcc gcctctttga tgcgggtgaa cccttcctgg
     6601 tacatgtcct tgaggtccgg actccaggtc gcatcccagg tatcgacttc ttttctggtc
     6661 tttccccaaa tgggcgtggc aatggcgcct ggcgtaatga gggcgacgcg gatgccccat
     6721 ggttgcagtt caaggcgcaa cgcatcggtg atcgcttcca gcgcatgttt ggaggcggcg
     6781 tagggggcca tgaacggcat ggaagctcgt ccggcgatgg agcccatgtt gatgatgcgt
     6841 ccgtgacccg tgcggatcaa cggcaaaaac gtctgggtca ccgcgatcag tccgaacacg
     6901 ttcacctcga attgccgtcg ccaatcggcg aggggcagga gttcaatggg accggccacg
     6961 ccgatgccgg cattattcac caggcctgca aggccgcgat cgcctaccat ggcggcgacc
     7021 gtatggctcg cggctgcgat cgacgcagag tcggtgacat cgagacgaat gggcatgagc
     7081 cgtggaccgg cttgccgctg cagacgttcg ccgtccgcgg gatgacgaac acaggcgaag
     7141 acgcggtacc cgagtgtgtc caacgcgagg gcgcaggcgg ctccgatgcc cgatgaggca
     7201 ccggtgatga ccacggagtc tgacggggaa gaggttgaaa accagttgag catgtctcgc
     7261 atcccttgtg tctggccgcg taccctacca tactttacgg tgtgacccta caatcagatg
     7321 tatccgatga cgtgcgagag gcgctgtgcc ttcggcgtag aacaagcaaa cgctcgagcg
     7381 atgagaggag caggcatgca cacagagaaa tgcaaatggg cgctgctgct tatgatggga
     7441 ctgagcctgg ctgcatacgt gtctcccctg aacgcaggca cggatcagaa gggagtcgtc
     7501 gccacggaga agcttgtcac ggtgcagccg cgggaaatcg acgacgtctt agacaatccc
     7561 gggatgggat ttgccgactt tcacttcggc ttcggccatc ctccgaagcc tgatgaatat
     7621 ccacatacga ccgtggccta tttccgttgg ccctgggcag agctggaacc ggccgagggc
     7681 cagtacaatt ttgcattggt ggacggagtc atcgcacagg cgaaggctaa gggtgagacc
     7741 ctcgccatcc ggatcgtctc agaatataag accggcaccc cgcaatggtt gctggataag
     7801 ggagtggcca gcatcaaaga aacggacggg atttttcccg actataacaa tcccatcttc
     7861 ctcgactacc acgaacgtct cattcgcgcg ttcggcgagc ggtatggccg gtcgctcgac
     7921 atcgatcatg tggatattgg atctgtcggg tgttggggtg aatggaatac ggcgtgctgt
     7981 gtcggcgcag agacgcagtg taaggcctat taccccactg aagcgaatca gctcgccatt
     8041 accgattggt atcttaaata tttctccgga actccccttg tgatgcttca cggaggacaa
     8101 ttgcaatatg cggcctcgcg aggggccggt tggcgggctg attgcttcgg tgactacggc
     8161 tatttctctc ccgactggaa tcacatggag catgcctatg ccccagtcct ggaggcgcag
     8221 gtagtggccg acgcgtggaa acaggggccg gtacagatgg aagtctgcgg ctatatccat
     8281 gaatggtacg aacggggctt cgatctcgac cgcatcctcg agaaggggtt ggaatggcat
     8341 gtctcggtgt tgaacgccaa gtcaaaaccg gtacccgccg cctggcggcc gcgcttcaat
     8401 gagtttctga aaaaaatcgg gtatcgattt gtgctgcgtg agctgaccca cccggcctcg
     8461 atcggagccg gtgaatcgct catcattcac tcacggtggg acaataaagg ggtggcgccc
     8521 atctaccatg cctggccgct ggcgtaccgg ttgcgttcgg gcacggatga agtggtcgcg
     8581 cagtggacca gcgctgccga tctgaagcgg tggctgccgg ggccttcgca cctggctgag
     8641 gatgcggtgg tggtgccgag caccgttcct gccggcgtct atacgctcga tgtcggaatt
     8701 ttatctgagg atgcgaagtc tgcgacggtc gagttagcga tagaaggcaa acggaccgac
     8761 cgctggtacg ccgtgtcgcg cgtggagatt cgataggctg tcggccgtcg tgcagatggg
//
LOCUS       DAOQEW010000158         8788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2671_length_8788_cov_6.200847, whole genome shotgun sequence.
ACCESSION   DAOQEW010000158 DAOQEW010000000
VERSION     DAOQEW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_8788_cov_6.200847"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..2102
                     /locus_tag="PLS35_14880"
     CDS             63..2102
                     /locus_tag="PLS35_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20005.1"
                     /translation="MTPSTALVPAEAQAFTPDLGRYRKWFTAYETNKQFEINEQLVHE
                     GYYNSTGHWTEEEARKLRKRGQAPIFDNRIARKIDFLVGVEQRMRRDPKGYPRTPNDE
                     QSADVATAGMRYACDINRWEFVGSAGTHDGLVRGIGVCFVGIKQGMTGPDPEIKSVQS
                     DRFFYDPRSKRPDFEDARYMGLHLWMDIDDAKEKWPDKAQQLQQIIDSVAAGGILSRA
                     DQNQETAWAEFESRRVRVVEFWEKTPRGWTYCYFVGEVMLDGGVSPYLDDEGKPDCPY
                     AAWSPYVDEKGNRYGPIRSMKPMQDEANHRRSKALHLFTTKQLHFRRGTLEDVDKTRS
                     ELAKPDGMIEHDGDWGNEVGIVDHSMDVAGQLQLLEQAQASLENLGPNPGLIGKGGGV
                     ADQSGRAILAQRDSGMTELSPVFERNRDWKLRVYRKLWSRIKQSWTAEKWIRITDEND
                     APQFIGLNQYNIDPQTGQAVSQNVVAMIDVDIIMEEGPDVITMNEELLQTLSQLGPNA
                     VPPKVLIELSNAPNKERLFKMIDEATQPDPVVQQMQQRMARLEQLVQASTVDKNVAQA
                     EYQRAQAVAALAKAFTPQQQKPMMDEMGNTIPQAPPAAPDMMAAMQALNMFPLQYRQP
                     TIEQYAENASPPQSPPDPDDQQQPEQPLPQQNAALPPPSGMPGGLPVNPQIAGMQ"
     gene            2117..2359
                     /locus_tag="PLS35_14885"
     CDS             2117..2359
                     /locus_tag="PLS35_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20006.1"
                     /translation="MKRLSAIARAHEVYDCLDRIGALRRHLTTLLGNHDAAVAGNAKR
                     DCNAFWHRLDELALRADATREVAAGHDRPFKDYKPE"
     gene            2496..3368
                     /locus_tag="PLS35_14890"
     CDS             2496..3368
                     /locus_tag="PLS35_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20007.1"
                     /translation="MTTEQSADDTLLNDVFSSGRERGADTAAPAEPEAKAAEPTAEPQ
                     SKEAAEPEAKPEATDAEPKGYRDPSTGRFVPLKELTSEREKRQSAEKARDEEARLRKY
                     AEDNWRRTQAQVEEIQRRIQAQAQPHQPPPDPNLDPAGAMAFQQEQFQQALLNQRLNT
                     SEMFARNKHGDQIVDAAFQAAQQAGVLRSFAYSKDCYGELVKWHRHQQVLARVGEDPD
                     AYEKTLEDKIRAKVLEELKTGNRAVMPGQTPPPVQQFPGSLADATGTGSNAAVPVSDE
                     AIMGNVFGSNRKRK"
     gene            3424..4536
                     /locus_tag="PLS35_14895"
     CDS             3424..4536
                     /locus_tag="PLS35_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HNI20008.1"
                     /translation="MATTPTLSGLELTKWRSKFITEYIRDTGFDTYMGDADTDIIHVV
                     NDLQTDGYTIRIPLMGKLGGSGVTGNTSLTGNEQQLDQYYQDITWEFRRQAVLATKKD
                     REKSAVDFMAQARPRLKEWSTENVKYRLIECFHKMSDGTPFSAADATTRNAFSAKNKD
                     RILYGSTQANYSGTHATGLTAIDNTDDKLSTKVGSLARFMARQARPMIRPYKTGTQGR
                     EFYVMFCHPLGFRDLKADTVMQQANRDARARDVESNPLFQDGDLIYDGVIYREIPEFY
                     QGNDTADAPNPETTFSNGTIQCGVSFLCGSQSIGFVNKQAAIPTTRADDDYGFLKGVG
                     IEFADGIDKLRWNNNPQGINNGKDVGIVTVYHAAVA"
     gene            4552..5007
                     /locus_tag="PLS35_14900"
     CDS             4552..5007
                     /locus_tag="PLS35_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20009.1"
                     /translation="MAIYATSQSLPGGKTKGPGASTQEVVIPFIVAVSTAMIDNANDE
                     VGLAWVPKGFMPTGIVFNTTDMDSSTGLLWDVGDDGDEDRLIAAFSGQAAGTSQTLRD
                     VGLLYKYTARTLIKAYVNTAATTGVAGTLKGVLKGIVDEDYDTTPLVAA"
     gene            5058..5273
                     /locus_tag="PLS35_14905"
     CDS             5058..5273
                     /locus_tag="PLS35_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20010.1"
                     /translation="MKFKFIGSEPSEFMGFKWYPGTEHDVTDDHAIRKLSNSVLFEKD
                     GTEANKEPKKRGAKPAQPVSDNGDDAN"
     gene            5281..5625
                     /locus_tag="PLS35_14910"
     CDS             5281..5625
                     /locus_tag="PLS35_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20011.1"
                     /translation="MATNVLLHFNIIAAEESPSASDSTYVIGRYRDLFNEMTLNDETY
                     WSIGQVPAEIFEPLTQMVALTVEKAFGKAAAMPPADYARSLDEGLRILRRRLRRTVNV
                     RSAEMPTYADDF"
     gene            5829..7046
                     /locus_tag="PLS35_14915"
     CDS             5829..7046
                     /locus_tag="PLS35_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20012.1"
                     /translation="MVEGALYVVAGVSLYKVTTTGVQTLIGSMNISKTAPVFMERNRR
                     AVPDIAIVCDGLMFYCRGDVLAQVTDPDLFAPITLAFSDGYFGITTAQNKWQIGAIDD
                     ASAWDGLDFATADGDPDALIRIAALQAQFYLFGEKTIEVWQDSGGADFPYARSFVIPI
                     GLLASNSVATVAETFAWVAHDRTVRLLGSGDSAQVISTPKVESDIQEVEDPSTIRAAS
                     WSSRGHTFYALTCPQWTHVYDTRMQRWHERQTYGRGNWRIAFVVPFGTKLIAGDADTG
                     ALFEMGPQFKDDAGDPLVSSVIPPTVHAFPQRVTHNALYIDVQRGVGTGQGDPQDVDP
                     EIMLEWSHDGGQTFSTQRMIKLGQQGRNLTRIRTHRLGQAPENGRVYRFSWSAKVDRA
                     LYAASADLEMDAA"
     gene            7043..7495
                     /locus_tag="PLS35_14920"
     CDS             7043..7495
                     /locus_tag="PLS35_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20013.1"
                     /translation="MTNTPLAPPPPRALLDPNGNVSDRFYQWLGSLQKGISGAFSGIG
                     EINAQQEAIDFTIESPDNQDYLFEIDCPFGYKITQVDSKCRSGTCTATTKIGSTPLGG
                     GANSVSTSLQTKTHTSANVIAAAGTSVVTISSNSSCAGLRLTFWITRT"
     gene            7492..8136
                     /locus_tag="PLS35_14925"
     CDS             7492..8136
                     /locus_tag="PLS35_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20014.1"
                     /translation="MRRRHICYSGAPPIVLTYLDAKGDSSGSTGAHTYNAAFGAASAN
                     RYILVGANITNPSVVDTIGGVAAVELFRVQAGTGTTVAAWYLAHVPTGTSGNITVTSP
                     GGGINWIGGVVVYSLISVSTPTLIDAVTGTNGVTNSWPMTAKSGSVALVLLAQGTPGK
                     VWSGGVTLDVALTFGGTGQIESASTHALIAGASITPSWNFGLAPAATSAAIVIK"
     gene            8180..8788
                     /locus_tag="PLS35_14930"
     CDS             8180..8788
                     /locus_tag="PLS35_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20015.1"
                     /translation="MGFFSDFTGASARSDINKANKNANAALDQGYNTSQGYYDKAASS
                     FDPYVSSGTAGQKMYGDLLGLNGTDARSSAQGIITSDPLWSGKLGEDTNAVLKNLNAR
                     GMGASGTAALAGQRVLLQNYNDVLDRYQGMGQTGLSATGQRAGVLTGQGDNAYGFGAT
                     KANQALQYGNAMAGTRNTGLNNVLAVLGTGAKAYGAYNGMKT"
BASE COUNT         1981 a         2616 c         2642 g         1549 t
ORIGIN      
        1 gcccaagcca gcggctacaa gcgattgaac gaggatagga atagcgacgg atggaagtct
       61 cgatgactcc aagcaccgcc ctcgttccgg cagaggcgca agcgttcacg cccgaccttg
      121 gccgataccg caaatggttc accgcctacg agacgaacaa gcagtttgaa atcaacgagc
      181 agcttgtcca cgagggctac tacaacagca cggggcactg gacagaggaa gaagcccgga
      241 agctgaggaa gcgcggtcag gcgccgatct tcgataatcg cattgcgcgc aagattgatt
      301 tcctcgttgg cgtcgagcag cgcatgcgcc gtgaccccaa aggctatccg cgcacgccga
      361 atgacgagca gtcggcagac gttgcgacgg caggcatgcg ttatgcctgc gacatcaacc
      421 gttgggagtt cgtagggtcg gccggaacgc atgacggtct ggtgcggggg attggcgtct
      481 gcttcgtcgg catcaagcag ggcatgaccg gacctgatcc cgagatcaaa tccgttcaga
      541 gcgatcgctt cttctacgac cctcgctcga aacgtccaga cttcgaagat gcgcgctaca
      601 tgggcctgca tctttggatg gacatcgacg acgccaagga gaaatggccg gacaaggcgc
      661 aacagctcca gcagatcatc gacagtgtcg ctgccggcgg cattctctcc cgtgctgatc
      721 agaaccagga gacagcgtgg gcggagttcg aaagccgccg cgttcgcgtc gtcgagttct
      781 gggagaagac accgcgcggc tggacgtact gctacttcgt cggtgaagtg atgctcgacg
      841 ggggagtttc gccgtatctc gatgatgaag gaaagccgga ttgcccgtac gcggcctggt
      901 cgccgtatgt ggacgagaaa ggcaaccggt acggcccgat ccgcagcatg aagccgatgc
      961 aggacgaggc gaaccatcgc cggtccaagg ccttgcacct gttcacgacc aagcagcttc
     1021 acttccgccg tggcacgctc gaagacgtcg acaagacgcg atcagaactc gcgaagcccg
     1081 acggcatgat cgagcacgac ggagattggg gtaacgaggt cggcattgtc gaccactcga
     1141 tggacgttgc cgggcagttg caattgctcg agcaggcgca ggcctcactc gaaaacctcg
     1201 ggccaaatcc tggccttatc ggcaagggtg gcggtgtcgc agatcagtcg ggacgagcga
     1261 tcctcgctca gcgtgactcg ggaatgaccg aattgtctcc cgtgttcgag cgcaacaggg
     1321 attggaagct gcgcgtctat cgcaagctct ggtcgcgcat caagcagtcc tggaccgctg
     1381 agaagtggat caggatcacg gacgagaacg acgctccgca gttcatcggt ctcaatcagt
     1441 ataacataga cccgcagacc gggcaggccg tgagccagaa cgtcgtcgcc atgatcgatg
     1501 tcgacatcat catggaagaa ggcccggacg tcatcacgat gaacgaggag ttgctgcaga
     1561 cgctttcgca gcttggtccg aatgcggttc cgccgaaagt gctgatcgaa ctctcgaacg
     1621 caccgaataa ggaacgtctc ttcaagatga tcgacgaggc gacacagccg gacccggttg
     1681 tgcagcagat gcagcagcgc atggctcggc tcgaacagct ggttcaggca tcgacggtcg
     1741 acaagaacgt ggcgcaagcc gagtatcaga gagcccaggc cgttgcggcg ctcgcgaagg
     1801 cgttcacgcc acagcagcag aagccgatga tggacgagat gggcaacacc atcccgcaag
     1861 ctccgccagc agcacctgac atgatggccg caatgcaggc gttgaacatg ttcccgctgc
     1921 aatatcgaca accgacaatc gagcaatacg cggagaacgc gtcaccgcct cagtctccgc
     1981 ctgatccaga cgatcagcag caacccgagc aaccgttgcc gcaacagaac gcagccttac
     2041 cgcctccgtc aggaatgccg ggcgggttac ccgtcaaccc acaaatcgca gggatgcaat
     2101 gagcctcgac gccgaaatga aacgcctaag cgcgattgct cgggcacacg aagtttacga
     2161 ttgcctcgac cgcattggcg ccctccgtcg gcatctcacg acgctgctcg gcaatcatga
     2221 tgcggcggtc gccggtaacg ccaagcgcga ctgcaacgcg ttctggcatc gtctcgatga
     2281 actcgcgctc agggcggatg cgacgagaga agtcgccgcg ggccacgacc gtcccttcaa
     2341 ggattacaag ccagagtaaa tgtagtgttt cgtgcgtagg cctcgccggc cgaaccggcg
     2401 tttcgcgacc atccgcgcgt aatcggatga acctcgcacc agccgggcga catcggctgc
     2461 tttcgtgacc agcaacgata ctgcggagag agacaatgac gactgaacag tcggcagacg
     2521 ataccctttt gaatgacgtg ttttcgagtg gccgcgagag gggggcagat accgccgctc
     2581 ccgccgagcc ggaagccaag gcagcagaac cgacagccga accgcaatcg aaagaagcgg
     2641 cagagccaga ggcaaagccc gaagcaaccg acgcggagcc caaaggatac cgcgatccat
     2701 caacagggag atttgtccct ctcaaagagc tgacctcaga acgtgaaaaa cgccagtcgg
     2761 cggaaaaagc acgcgatgaa gaagctcgtc tgcgcaagta tgccgaagac aactggcgcc
     2821 gcactcaggc ccaggtcgaa gaaatccagc gcagaattca ggcgcaagcc caaccgcatc
     2881 aaccgccgcc cgatccgaac ctcgatccgg ccggcgccat ggcgttccag caggaacaat
     2941 tccaacaggc tctcctcaac cagcgcttga acacctccga gatgtttgcc cggaacaagc
     3001 acggcgatca gatcgtcgac gccgcgtttc aggcagcgca gcaggccggt gtcctacgtt
     3061 ccttcgctta cagcaaggat tgctacggcg aactggtgaa atggcaccgc catcagcagg
     3121 tcttagcccg agtgggtgaa gaccctgacg cctatgagaa gacgcttgag gacaagatcc
     3181 gcgccaaggt gctggaagaa ttgaagaccg gaaaccgggc agtgatgccg ggacagacgc
     3241 cgcccccagt acagcagttc ccaggctccc tcgcagacgc gaccggaacg ggatcgaacg
     3301 ctgctgttcc cgttagcgac gaagccatca tgggcaacgt tttcggttca aaccgaaagc
     3361 gaaagtaagc cgcatcagac gcgcttcgtg atccccaacc cctgatcacg aaagatctcc
     3421 aacatggcaa ccactccgac cctttccggg ctcgagctta ccaaatggcg ctcgaagttc
     3481 atcacggagt acatccgtga caccggcttt gacacctaca tgggcgatgc cgacaccgac
     3541 atcatccacg tcgtgaacga cctgcagacg gacggataca cgatccgcat ccctctgatg
     3601 ggcaagctcg gcggctccgg cgtcaccggc aacacctcgt tgaccggcaa cgaacagcag
     3661 cttgatcagt attaccagga catcacctgg gagtttcggc gacaggccgt gctcgcgacg
     3721 aagaaggacc gagaaaagtc cgccgtcgat ttcatggccc aggcgcgccc gcgtctgaaa
     3781 gaatggtcga ccgagaacgt caaataccgg ctgatcgagt gcttccataa aatgtcggac
     3841 ggcacgccgt tctcggcagc cgatgcgacc acgcgaaacg cgttctctgc caagaacaag
     3901 gatcgcatcc tctatggcag cacacaggcg aactactcag gcacgcatgc gaccggcctg
     3961 accgcaatcg acaacacgga cgacaagctg tcgaccaagg ttggctcgct ggcgcgcttc
     4021 atggcccgcc aggcgcgccc gatgatccgg ccctacaaga ccgggacgca gggccgcgag
     4081 ttctacgtca tgttctgcca cccgcttggc ttccgcgatc tcaaggcgga cacagtcatg
     4141 cagcaggcca accgtgacgc acgtgcgcgc gacgtggagt ccaacccgct cttccaggac
     4201 ggcgacctga tctacgacgg cgtcatctac cgtgaaatcc ctgagttcta tcagggcaac
     4261 gacaccgcgg acgctccgaa ccctgagacg acgttctcga acggcaccat tcagtgcggt
     4321 gtttcgttcc tgtgcggctc tcagtcgatc ggcttcgtca acaagcaggc cgcgatcccg
     4381 acgacccgtg cagacgatga ctacggcttc ctgaaaggcg tcggcatcga gtttgcggac
     4441 ggcatcgaca agctgcggtg gaacaacaac ccgcagggca tcaacaacgg caaggacgtt
     4501 ggcatcgtca ccgtctacca cgcggccgtg gcctaaagga gaacccgaac aatggctatt
     4561 tacgcaacat ctcagtctct tcctggcggc aagaccaaag gacctggcgc ttccacgcag
     4621 gaagtcgtca tcccgtttat cgtggctgtt tcgacggcca tgatcgacaa cgccaacgat
     4681 gaagtcggat tggcttgggt gccgaaaggc ttcatgccga ccggcatcgt cttcaacacg
     4741 accgatatgg actcatccac gggcctgctg tgggacgttg gcgatgatgg tgacgaagac
     4801 cgtctcatcg cggcgttttc cgggcaggcc gcgggcacgt ctcagacgct tcgagatgtc
     4861 gggctcctct acaagtatac ggcccgcacg ctgatcaaag cctacgtcaa caccgctgcg
     4921 accacaggtg tcgcggggac gctgaaaggc gtgctcaagg gcattgtcga cgaagactac
     4981 gacacgaccc cgctggttgc ggcctgatga aacgagagcg gcgggcaact gccgctcttt
     5041 ctctttgagg agatcagatg aaattcaaat ttatcggtag cgagccgtct gagttcatgg
     5101 ggttcaagtg gtatcccgga accgagcatg acgtgacgga cgatcacgca atccggaagc
     5161 tttccaacag cgtcctgttt gagaaagacg gaacggaagc gaataaagag ccgaagaagc
     5221 gcggcgccaa gcccgctcaa cccgtgagcg acaatggcga cgacgcgaac tgagctagag
     5281 ttggcgacga atgtcctgct gcatttcaac atcatcgcag cggaggagtc gccgtcggcc
     5341 agcgacagca cgtacgtcat cggccgctat cgcgacctgt tcaacgagat gacgttgaac
     5401 gacgagacgt actggagcat cgggcaggtg ccggccgaaa tcttcgagcc gctcacccaa
     5461 atggtcgccc tgacggtcga gaaggcgttc gggaaggcgg ccgccatgcc gcctgctgac
     5521 tacgcccgca gcctggacga agggctgcgc atcttgcggc ggaggctgcg ccgaacggtc
     5581 aacgtgcgct cggccgaaat gccaacctat gcggatgatt tctgatgggc atcgttcctc
     5641 tcgcgcttcc gtccggatca agccaggccc ggttcaacca gggtggctcg gcgcaactca
     5701 tcaactgcta tctctcaccc attggcgagg aggggaaggt caagaacgca atctattcct
     5761 ctgatgggtt gcaaggtttc gcgctgctgc aaggcgtggc ggcggggcag ggatgccggg
     5821 ccggtatcgt ggtggaaggc gccctgtacg tcgtcgcagg cgtgtcgctc tacaaggtga
     5881 caacgaccgg cgttcagacc ctcatcgggt cgatgaacat ttccaagacc gctccggtct
     5941 tcatggaacg aaaccgcaga gcggtgcccg acatcgcgat cgtgtgcgat ggcctgatgt
     6001 tctactgccg gggtgatgtg ctggcgcagg tgaccgaccc cgatctgttc gcgccgatca
     6061 cgctcgcatt ctcggacggg tatttcggga tcaccacggc gcagaacaaa tggcaaatcg
     6121 gggcgattga cgacgccagc gcatgggatg gactggactt tgccacggcc gatggcgacc
     6181 cagatgctct gatccgcatc gcggcgctgc aggcgcagtt ctatctcttt ggcgagaaga
     6241 caatcgaggt ttggcaggac agcggtggcg cggacttccc atacgcccgg tcattcgtca
     6301 ttcccatcgg gttgctcgcg tcaaattcgg ttgcaacggt tgccgaaacg tttgcctggg
     6361 ttgcccatga tcgcacggtc cgattgctgg gctcgggcga cagcgctcag gtaatctcga
     6421 cacccaaggt cgaaagcgac atccaggaag tcgaagaccc gtcaacgatc cgggccgcgt
     6481 cctggtcgtc acgaggtcac acgttctatg ctctgacctg cccgcagtgg acgcacgtct
     6541 acgacacgag aatgcagcgg tggcatgagc gtcagaccta cggccggggg aattggcgta
     6601 ttgccttcgt tgttccgttc ggcacgaaac tgatcgcagg agatgccgat accggcgcgc
     6661 tctttgaaat gggcccgcag ttcaaggacg atgcaggcga tccgttggtg tcatccgtca
     6721 ttccaccgac ggttcatgcg ttcccacaga gagtgacgca caacgcgctc tatatcgacg
     6781 tgcagagggg tgttggaacg ggccaaggcg atccgcagga cgtcgacccg gagattatgc
     6841 tggaatggtc gcatgacggc gggcagacgt tcagcacgca acggatgatc aagctcggac
     6901 agcagggtag gaacctgaca cgcatccgta cgcatcggct cggacaggcc ccggaaaatg
     6961 gccgggtgta ccggttctcg tggtcggcaa aggtcgaccg ggcgctctat gccgcttctg
     7021 ctgatcttga aatggatgcg gcatgacgaa tacgccgctc gctccgccgc caccacgggc
     7081 cttgctcgat ccgaacggaa acgtcagtga ccggttctat cagtggcttg ggagtctgca
     7141 gaaggggatt tctggggcgt tctcgggcat tggagaaatc aacgctcagc aggaagccat
     7201 cgacttcacg atcgagagcc cggacaacca ggactatctg ttcgagatcg attgcccgtt
     7261 cggatacaag atcactcagg ttgacagcaa atgcaggtct ggcacctgca ccgcaacgac
     7321 gaagattgga agcacacctc tgggaggtgg agcgaactcg gtttcaacct cgcttcagac
     7381 gaagacgcac acttcggcca acgtcattgc agcggcagga acatcggtcg ttacgatctc
     7441 atccaatagc agttgtgccg gtcttcgcct gacgttctgg attacacgca cgtgaggcgg
     7501 cggcatattt gctatagcgg agcgccgcca attgttctga cgtatctgga tgcgaagggg
     7561 gacagttccg gaagcaccgg cgcccatacc tataatgccg cgttcggtgc ggcgtccgca
     7621 aatcgatata ttctggttgg ggcgaacatc accaacccgt cggtcgtgga cacgatcgga
     7681 ggggtagcag ccgttgagct tttccgggtg caggccggga ccgggaccac ggttgcagcg
     7741 tggtatttgg ctcatgttcc aaccggaaca tccggcaaca taaccgtaac gtcacccggt
     7801 ggcggtatca actggattgg cggtgttgtg gtctattcgc tgatctccgt ctcgacgcca
     7861 acactaattg atgcggtgac gggaactaac ggcgtcacga attcgtggcc catgacagca
     7921 aaatccggat cggtcgctct ggtcctcttg gcgcaaggaa cgccgggaaa ggtttggagc
     7981 gggggcgtca ctctcgatgt cgcgttgaca ttcggcggta ctgggcaaat cgaatccgcg
     8041 agcacgcatg cactcatcgc aggcgcatcg attacgccgt catggaattt tgggctcgcg
     8101 ccagcggcga cgtctgccgc gatcgtaatc aaatagcgtt tcgctagccg cctccctctg
     8161 actgaataag gaaaatccca tggggttctt ttcggacttc acgggcgcct ctgcgcgctc
     8221 cgatataaac aaggccaaca agaacgccaa cgcggctctc gatcagggct acaacacctc
     8281 gcaggggtac tacgataagg cggcaagctc ttttgatccg tatgtgtcgt caggaaccgc
     8341 cggtcagaag atgtacggcg atcttctcgg gctcaatggc accgatgcga ggtcatcggc
     8401 tcagggcatc attacgtccg acccgctctg gtcgggaaag cttggtgaag acaccaatgc
     8461 cgttctgaaa aacctcaatg ctcggggcat gggtgcttcc ggcaccgcgg cgctcgccgg
     8521 tcaacgtgtt ctgcttcaaa actacaatga cgtgctcgac cgatatcagg gcatgggcca
     8581 gacgggcctc tccgccacgg gacagagagc aggcgtcctg accggacaag gcgataacgc
     8641 ctacggcttc ggagcgacga aagcgaacca ggcgctccag tacggaaacg cgatggccgg
     8701 aacacgaaac actggcctta acaacgtcct cgccgtgctc ggaaccggtg ccaaggctta
     8761 cggcgcctac aacgggatga agacctga
//
LOCUS       DAOQEW010000159         8770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2681_length_8770_cov_9.028112, whole genome shotgun sequence.
ACCESSION   DAOQEW010000159 DAOQEW010000000
VERSION     DAOQEW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_8770_cov_9.028112"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..662
                     /locus_tag="PLS35_14935"
     CDS             99..662
                     /locus_tag="PLS35_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20016.1"
                     /translation="MAAPRIIDYGRIEPGWREGVKSLPMLCAEYEDATGQKVTPAAVR
                     KHFVKLGIPRDLSAKIEAKAAAMVSAAMVSGKVSVTGNETLPAEAAIIASAATTSANV
                     QLSHRADIRLLRMRVAEYQEELSECDGDLAKRTSILKSLAETQCRLISAEREAFGMDK
                     DRPADTGLSGESVAMLRRMKAEIESRV"
     gene            646..2052
                     /locus_tag="PLS35_14940"
     CDS             646..2052
                     /locus_tag="PLS35_14940"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNI20017.1"
                     /translation="MKVESELLAAIEKELSRRRLEAYEPYARQAEFHAAGATHRERLF
                     CAGNQLGKTLAGAAEMAIHLTGRYPENWRGRRFQRAIAAWASGVTGESVRDTTQRLLV
                     GRPGEYGTGMIPAACIVGEPKRALGVADLLDSVTVRHVSGGNSRLYFKRYEQGREKWQ
                     GETLDVVWFDEEPAPEIYTEGLTRTNATGGMAYMTFTPLQGMSEVVMRFLHEQSPDRH
                     VTYMTIDDAEHYTPEQRAAIVASYPAHEREARAKGIPTLGSGRIFPVEEESILVEQFL
                     IPAHWPRINGIDFGWDHPAAAVQAAWDRDADCWYVIKAHRAREQTPILFAPTVKAWGA
                     WVPCAWPHDGLQHDKGSGKGLAQQYADTGLNMLREHATHAPDPGKPEGSGGNGVEAGL
                     MEMLDRMQTGRLKVFAGLDDWMQEFRLYHRKDGRVVKERDDLMSATRYGLMMRREAIV
                     QPDPYTRPSARRPACWRA"
     gene            2883..4280
                     /locus_tag="PLS35_14945"
     CDS             2883..4280
                     /locus_tag="PLS35_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20018.1"
                     /translation="MVEYDRKNSAHVVAVAKGLVSVDSAIVSRVRLSWWLGPHKLADV
                     DHPEQHGKFPYVPFWGMREDRTGVPFGLARGMVYLQDEINATRSKAMWMMAARRVERT
                     QGAVVGEDEQFRQEVARPDADIILDPKAMREGGIFKVSTDLELTQQQFQRLADAREAI
                     RRVGGIYSEFQGQNSNTTSGVQFNSQVEQSNQSLADILDNFKTARTEVGEILLSLIIA
                     DTIGKQEDVFLDGGGLVDDRTIGINVPVVDDDGFEYLDNDVSRVALTVGIDDVPSAAT
                     FKQQQLNAMTEAFKVMPVQYQSVMIPFLLSLMDLPNSADLIKEIRAAASASSPEQVEQ
                     QIKQAVEQALVKARAEIELEKLRQNKTLIDAQVSKLAAEAATKAVEGFFSATQAANQI
                     AMLPGLAQSADQILRSAGMPDRDAAPLIAPVPNGTTPSADLQQNTSPMFPANPDVGLK
                     TGIEGGQPSSSEGQP"
     gene            4277..4516
                     /locus_tag="PLS35_14950"
     CDS             4277..4516
                     /locus_tag="PLS35_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20019.1"
                     /translation="MSSLQETAVRYGAVTPSDSTELKFKALYIGGAGSVVIRQAGSDI
                     GAVTFAAVPAGTQLLVSGNRVMAASGATNIVWLDW"
     gene            4707..5645
                     /locus_tag="PLS35_14955"
     CDS             4707..5645
                     /locus_tag="PLS35_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20020.1"
                     /translation="MEKDLSYFMEHPEEFDQLSDEDRMSLHIGESIEGEITGGSPAAS
                     IEASAEEGATDKTHTAAEEAPVVVAKDGKHTIPFEELQRARDEAKYWQAQAEEASRIA
                     QQSQSEQPHNQTAAVDLKQLRRELREAMLLDDDARIDELEAQIDEEISRKAEAKAIQV
                     LEQQAAQAEERAISATAESLIKTYPDLDHTKPGANLEAIATVQALSAMYVNKGSTRAQ
                     ALVDAVDRVAKLYGMGEKAKADLPDEAAMAKAEKVIAQASAKAKVPSSLSSIPAAATP
                     PADELQALSSMSVQQVQDKLMSMPREKILAVLSRQM"
     gene            5705..6973
                     /locus_tag="PLS35_14960"
     CDS             5705..6973
                     /locus_tag="PLS35_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HNI20021.1"
                     /translation="MATTAVPYGSDQQVKIQSVALFAACMQRKTGLNRMSGAMSKQAD
                     ASGNIRLVSTNKKPIVRVQELTKSAGDEVTFDLVNPVKAIPIMGDEWAQGKGSVLTFA
                     QDRLRINQARFPISAGGAMSQQRTPHQLRPLAQDVALSSLERFSDQASLVHLAGARGF
                     HDNIEWCVPTTANADFSKVMVNPVRAPTRNRHFLSTGSGLEQVPTGGYTIATTDVMNS
                     DVVDGIATWLDGMALPLPGVEFENDEAAADSPVRVLMVSAEQYNSFVQSTNFRTLQAQ
                     AISRAQMAKNHPVFLGDALLWRGILILKMPRPIRFYPGDTVQWCASTTSATETAGTIP
                     ALGSGYAVDRAILLGGQALAEGFGRHNGSGSSYFTAEETTDFGNQREYVVGEIGGRSK
                     IRFLIDHGNEQQYTDYGVAVIDTAVRLAGV"
     gene            6987..7466
                     /locus_tag="PLS35_14965"
     CDS             6987..7466
                     /locus_tag="PLS35_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20022.1"
                     /translation="MATVTKKSLRQEVSHAGAFGNLWSQIYTFETNSSGIFVNSDLAT
                     AVQIGDVVRFGILPAGIKISDTLVIISDAFATSTTYKLGFAYVDGVDSTAVPQDDDYF
                     IVAGTSSASAARTPANNTAVRPITLPKEAYIILTRTGAADSAVGIMDVMVKGTLVGV"
     gene            7513..7902
                     /locus_tag="PLS35_14970"
     CDS             7513..7902
                     /locus_tag="PLS35_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20023.1"
                     /translation="MHAQTIVYIGRRPYHKDCTYGTGEWVNGQSKVVDAAIAFKMLRH
                     PDVYQDGGVVADGDVSDPVTTQKKATPEHETEETLWALDAINTMDTDALCSFVAQNFN
                     QKLDRRKSLNNLRLDAENMVHQFGVAS"
     gene            7899..8540
                     /locus_tag="PLS35_14975"
     CDS             7899..8540
                     /locus_tag="PLS35_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20024.1"
                     /translation="MNLGTLISNFRIDERDTSTPPMLGRDHLVRLFNEATEEAAIRKS
                     LLRENLEFVLSAGDSEVSLPPRIIEVRTARIVEGGATYWLSPSSRHEQDRLFRNWRDT
                     VERPSAFIHDDISITLNRIVLASSVLKLECCRTPVNPMEIDEDEPEIAAVHHRYLDGW
                     VRYRAYRVPDADFMDKDRSDRGLADFEDYFGRRPTADHRRDNNADRPHRVKAW"
BASE COUNT         1987 a         2388 c         2761 g         1634 t
ORIGIN      
        1 tgggcgctgc tgctgctgcg caggcatggg cttgtggagg cggtgccaga tcctgtccgg
       61 aatgggcggt acttgcggta caaggcgagg gcggtctgat ggcggcgccg agaataatcg
      121 actacgggag gatcgagccg ggatggcgcg agggcgtcaa aagcctgccg atgctgtgcg
      181 cagagtatga ggacgcgaca gggcagaaag tgactcctgc cgcggtacga aagcacttcg
      241 tcaagcttgg catcccccgc gacctgtcgg caaagatcga ggcgaaagcc gcggcgatgg
      301 tatcggcggc gatggtatcg ggcaaggtat cggtaacggg aaacgaaacc ttgccggcag
      361 aagcagcgat catcgccagc gcagcgacga caagcgctaa tgttcagctt tcgcaccggg
      421 cagacatccg gctgctgcga atgcgagtcg cagagtatca ggaggagctg agcgagtgcg
      481 atggcgacct ggcaaagcgc acctccatcc tcaagtcgct ggccgagacc cagtgccggc
      541 tgatctcggc agagcgcgag gctttcggca tggacaagga tcgaccggca gatacgggac
      601 tgtcaggaga gtcggtagca atgctcaggc ggatgaaggc ggaaattgaa agtcgagtct
      661 gagttgctcg cggcgatcga gaaggagctt tctcggcgtc gccttgaggc atacgagcca
      721 tacgcgcgcc aggcggaatt tcacgcggca ggagctacgc atcgagaacg gcttttctgc
      781 gccgggaatc aactcgggaa aactctcgcc ggagccgcgg aaatggcgat tcacctgacc
      841 gggcgctacc cggaaaattg gagaggccgc agattccagc gcgccatcgc ggcatgggcg
      901 tcaggagtaa ccggcgagtc tgtgcgagat acgacgcagc gtttgctagt cgggcgtccg
      961 ggagagtacg gcacagggat gattccggca gcgtgcatcg tcggcgagcc aaagcgggca
     1021 ttgggagtcg ccgatctgct ggacagcgtg actgtccggc acgtcagcgg tggcaacagt
     1081 cggctgtatt tcaaacgcta cgagcagggc cgggagaagt ggcaagggga gacgctcgat
     1141 gtggtttggt tcgacgagga gccggcgccg gaaatataca ccgagggcct gacgcggacg
     1201 aatgcaaccg gcggcatggc gtacatgaca ttcacgccac tgcaaggcat gtcggaagtg
     1261 gtcatgcgat tcctgcacga gcaaagccct gatcgtcacg ttacgtacat gaccatcgac
     1321 gacgcggagc actacacacc ggagcagcgc gcagccatcg tcgcatccta tccggcgcat
     1381 gagcgtgagg ctcgcgcaaa gggcatcccg acactcggca gcgggcgaat tttcccggtc
     1441 gaggaagaat cgatcctcgt cgaacagttc ctaattccgg cgcactggcc gcgtatcaac
     1501 gggattgact tcgggtggga tcacccggca gccgctgttc aggcagcatg ggatcgagac
     1561 gctgattgct ggtacgtgat caaggcgcat cgtgcgcgcg agcaaacccc cattctgttc
     1621 gcccctacag tcaaggcatg gggcgcgtgg gtgccgtgcg cctggcctca cgacggcttg
     1681 cagcacgaca aagggagcgg gaaagggctc gcgcagcagt acgcagacac agggttgaat
     1741 atgctcaggg agcacgcgac tcatgcccca gatcccggga agccagaggg gtctggcggc
     1801 aatggcgtcg aggctgggct gatggaaatg ctggaccgga tgcaaaccgg gaggctgaaa
     1861 gtgttcgcag ggttggacga ctggatgcag gagtttcggc tgtatcacag aaaagatggg
     1921 cgagtcgtga aagagcgcga cgacctgatg agcgcgacga ggtacgggtt gatgatgcgt
     1981 cgggaagcga ttgttcagcc agatccgtac accaggccat cagcacgtcg cccggcgtgc
     2041 tggagagcat agaaatcaat catggtatat ctgcatctga atagcatggc gaacgcatga
     2101 tgtcagaaga actttccctc gacgagtaca ccggattctg gacagaaatt caggagcagc
     2161 cgccgtggcg cacgaaggct gatcgagagg ctgattacat cgacggcaat caactggacg
     2221 ccgagattct gcagcgaatg gccgatatag gcatcccgcc ggccatcgaa ccgctgatgg
     2281 ggcctgtact ggcatcggtc cttggcatgg aagtgcggaa tcgcggggac tggatcgtgc
     2341 ggccgcagtc gcaggacgac agctcggatg tggcagacgc gctgagtttc aaactgcacc
     2401 aggcggaaca gcggtcaaaa gcggatgtcg cgtgctcaga ggcgttcaag acacagatcg
     2461 gcctcggaat aggttggtgc tatgtgggtc gggaagaaga tccgttccgg tatccgtaca
     2521 aagtcgaagc ggtccaccgg aatgagattt tctgggattg gttcgcaaag ccggaccttt
     2581 cgaatgcccg ctatctgatc cgccggcggt ggttcgacaa gcgcattcca gagctgattt
     2641 ttccgaagca tgccaagttg atcaggcaca cgacgagcgg atggtcagat tacggcgtgg
     2701 gtcatttcat gaccgattcg gccgggtcgc tgcctaatct gttcgccagc caggaacagg
     2761 agcgcacatg gtcaatcgag gaatcgcagt ggcgcaactc gtcgcagaac cgggtggccc
     2821 tctgcgagtg ctggtatcgt cgatggaatc gcgtcaccgt catcagatct ccggacggca
     2881 gagtggtcga gtacgaccgg aaaaactcgg cgcatgtggt cgcagtggca aaaggtctgg
     2941 tgtcggtcga tagcgcgatt gtgtcgcgag tgcgattgtc gtggtggctc gggccgcaca
     3001 agctggcgga tgtcgatcat ccagagcagc acggaaaatt tccgtatgtc ccgttctggg
     3061 ggatgcgcga ggaccggaca ggggtgccgt ttgggctggc tcgcggaatg gtctatctgc
     3121 aggatgagat caacgcgacg cgcagcaagg ccatgtggat gatggccgcg cgccgcgtgg
     3181 aaaggactca gggcgcggtg gtcggggagg atgagcaatt caggcaggaa gtcgcgagac
     3241 cagacgcgga catcattctt gacccgaaag ccatgcgtga aggcggcatt ttcaaggtct
     3301 caaccgacct agaactgacg cagcagcaat tccaacggct agcggacgct agagaggcta
     3361 ttcggcgagt cgggggaatc tactctgaat tccaaggaca gaactcgaac acaaccagcg
     3421 gcgtgcaatt caacagccag gttgagcaga gcaatcagag cctcgccgac atcctggaca
     3481 atttcaagac agcgcgaact gaagtgggcg aaattctgct gtctctgatc atcgcggaca
     3541 cgatcgggaa gcaggaggac gttttcctag atggcggcgg gttggtcgat gaccgcacga
     3601 tcggaatcaa tgtgccggtc gtcgatgatg acggtttcga gtacctcgac aacgacgttt
     3661 ctcgcgttgc gctgacagtc gggattgacg acgttccttc agcagccaca ttcaaacagc
     3721 agcagctcaa cgcgatgacg gaagctttca aagtcatgcc ggttcaatat cagtcggtaa
     3781 tgatcccgtt cctgctgtcg ctgatggatt tgccgaatag tgcagatctg atcaaggaaa
     3841 tccgtgcggc agcgtctgcg tcctcgcctg agcaggtcga gcagcagata aaacaggctg
     3901 tcgagcaggc gctggtcaaa gcccgcgcgg aaatagagct ggaaaaactt cgccagaaca
     3961 agacgctgat cgacgcgcag gtcagcaaac tggccgccga agcagcaacc aaggccgtcg
     4021 agggattttt ctcggcaaca caggcggcca accagatcgc catgctgcca gggctcgcgc
     4081 aatccgctga tcagatattg aggtctgccg ggatgcccga ccgcgacgcg gcgccgctta
     4141 tcgcgccagt tccgaatgga acaacgcctt cggctgacct tcagcaaaac acctcgccga
     4201 tgttccctgc caatccagat gttggcctga aaacagggat agagggcggt caaccatcat
     4261 ccagtgaagg acaaccatga gttcattgca agaaaccgct gttcgctacg gcgccgtgac
     4321 gccaagcgac tcaaccgagt tgaaattcaa ggcgctgtac atcggaggcg ccggatctgt
     4381 agtgatccgg caggctgggt ctgacatcgg ggcagtgacg tttgccgccg ttcctgcggg
     4441 cacgcaactc ttggtgtctg gaaatcgcgt gatggccgcc agtggcgcaa ccaatatcgt
     4501 ctggctcgat tggtagtcaa aatcaatcat gctaaaaagg cagcagcccg ccgtgatggc
     4561 gcgcgatccg cagcagatgg aggcggaggc ccttcggggc caatgccatt ttcgtaacac
     4621 ccgcttacat ctcgcccgca gcacaagcga tatgtgaccg gatagcccgc cgtgatggcg
     4681 cgcgagtcct gtagatggaa cggtagatgg aaaaagattt gtcctacttc atggagcacc
     4741 ctgaagaatt cgaccagctt tcagacgaag atcgtatgtc gctgcatatc ggcgagtcga
     4801 tcgagggcga gattaccggc ggatcgcccg ccgccagcat tgaagcttca gccgaagaag
     4861 gtgcgacaga caaaacacat acagccgcag aggaagcccc tgtcgtggtt gccaaggatg
     4921 ggaagcacac gatcccgttc gaggaactgc aacgagccag agacgaggcc aaatactggc
     4981 aggcccaagc cgaggaagcc agccggatcg ctcaacagtc ccaatcagag cagccgcata
     5041 accagacggc cgccgtcgat ctgaagcagc ttcgccgcga gcttcgcgaa gcgatgttgc
     5101 tggatgacga cgccaggatc gatgagcttg aggcgcagat tgacgaggag atcagccgta
     5161 aggctgaagc caaggcaatc caggtgcttg agcaacaggc ggcgcaggcc gaagagcggg
     5221 cgatttcggc gactgccgag agcctgatca agacatatcc cgatctggat cacacaaagc
     5281 ctggcgccaa cctggaagcc atcgcgaccg tacaggcgct gagtgcgatg tacgtcaaca
     5341 agggctctac tcgcgctcag gcgctggtgg acgctgttga tcgggtggcc aagctctatg
     5401 ggatgggaga gaaggcgaaa gctgaccttc cggatgaagc cgcgatggcg aaggcggaaa
     5461 aagttatcgc acaggccagc gcaaaggcaa aagtgccgtc cagcctgtcg tcgattcctg
     5521 cggccgcaac cccgccagca gatgagttgc aggcgctttc gtcgatgtct gtgcagcaag
     5581 ttcaggacaa gctcatgagc atgccgcgag aaaaaatact ggccgttctg tctcgacaga
     5641 tgtagccggc cattttgatt aaccgccgtg atggcggaat tttcctcata caggagcagt
     5701 aaaaatggca accacagcag tcccctatgg gtctgaccaa caagtcaaga tccagtcggt
     5761 cgccctgttc gctgcgtgca tgcagcgcaa gaccggcctc aatcggatgt ccggcgcgat
     5821 gtcaaaacag gccgacgcca gcggaaatat ccgactcgtc agcaccaaca aaaagccgat
     5881 cgtccgggtc caggaactca ccaagtcggc cggcgacgaa gtaaccttcg atctggtcaa
     5941 tccggtcaaa gccattccga tcatgggaga cgaatgggcg cagggcaaag gctccgtgct
     6001 aacgttcgcg caggaccggc tgcggatcaa tcaggctcga ttcccgatca gcgcaggcgg
     6061 cgcaatgtcg caacagcgca cgccgcatca gctccgcccg ttggcgcaag atgtcgcgct
     6121 gtcgtcactt gagcgattct ctgatcaggc atctttggtc catctcgccg gcgcacgtgg
     6181 attccacgac aacatcgagt ggtgcgtgcc gactacggcg aatgccgact tctcaaaggt
     6241 gatggtcaac cccgtccgag cgccaactcg taaccgacat ttcctctcga ccgggtcggg
     6301 cctggagcag gtgccaaccg gcggctatac gatcgccacg acggatgtga tgaactctga
     6361 tgtggtggat ggcatcgcca cctggctgga cggcatggcc cttcctctgc ctggggtcga
     6421 gttcgagaat gacgaagcgg cggctgattc gcctgtgcgc gtgctgatgg tgtccgccga
     6481 gcagtacaac tcgttcgtcc agagcaccaa tttccggacg ctgcaagcgc aggccatctc
     6541 tcgggcgcaa atggccaaga accatccggt cttcctcggc gacgcgctgc tctggcgggg
     6601 aattctcatc ctcaagatgc cgaggccgat ccgtttctat ccgggagata cggtccagtg
     6661 gtgcgcttcc accacctctg caaccgagac agccgggacg attcctgcgc tcggctcagg
     6721 gtacgcagtc gatcgcgcca ttctgctcgg cggccaggcc ctcgcggaag ggttcgggcg
     6781 gcacaacgga agcggaagtt catatttcac cgcagaagaa acgaccgact ttggcaatca
     6841 gcgtgagtac gtcgttggcg agatcggcgg gcggtcgaaa atccgctttc tgatcgatca
     6901 tgggaacgag cagcaatata ccgactatgg cgttgcggtg atcgataccg ctgtccggct
     6961 ggccggcgtt taaggaggtt tgacagatgg caacagttac taagaaatcg ctccgacagg
     7021 aagtatctca cgccggggca ttcggcaatc tctggtcgca aatctatacg ttcgagacca
     7081 attcttcagg gatattcgtc aactcggacc ttgcgacggc ggtgcaaatc ggtgatgtgg
     7141 tccggttcgg gattcttcct gccgggatca agatttccga caccctggtg atcatctcgg
     7201 atgcgttcgc gacatcgact acctacaagc tcggtttcgc ctacgtcgac ggcgtcgatt
     7261 ccacggcggt accgcaggat gatgactatt tcatcgttgc gggaacctca tcagcttcgg
     7321 ccgcgcgaac gcccgccaac aatacggctg tccgaccgat caccctgcca aaagaggctt
     7381 acatcatcct gactcggacg ggcgcggcag actcggctgt tggcatcatg gatgtcatgg
     7441 tcaaagggac tctggtcggc gtctaaccct atcgcgccgg cccaagccgg cgcatttcca
     7501 tggattcgca caatgcatgc acagacgatt gtctatatcg gacgcaggcc gtatcacaag
     7561 gactgcacct acgggactgg agagtgggtc aatggacaga gcaaggtcgt tgatgccgct
     7621 atcgcgttca agatgttgcg gcaccctgat gtctaccagg acggcggggt tgttgctgat
     7681 ggtgatgtgt cagatccggt cactacgcag aaaaaggcga ctcctgagca tgagactgag
     7741 gaaacacttt gggcgcttga cgcaatcaac accatggaca ccgacgccct gtgttcgttt
     7801 gtcgcccaga atttcaacca gaaactggac cgcaggaaat cgctgaataa ccttcgactc
     7861 gatgcggaga acatggtgca tcagttcgga gttgcgtcgt gaatctcggc accctgatct
     7921 ccaattttcg tatcgacgag cgcgatacga gcactccgcc gatgttgggg agagatcacc
     7981 ttgttaggct gttcaacgag gccactgaag aggcggcgat tcgtaaaagc ctgctgcgtg
     8041 aaaacctaga gtttgtgttg tctgccggtg attctgaggt ttcattgcca ccacgaatca
     8101 tcgaggtgag gaccgctcga atcgtcgaag gcggggctac ttactggctt tcgcccagca
     8161 gccggcacga gcaggacagg ttgttcagga actggcgaga caccgttgaa cggccgagcg
     8221 catttatcca cgacgacata tcgatcacgc tgaatcggat cgttttggcc tcttccgtgc
     8281 tgaaactgga gtgctgtcga actcctgtga atccgatgga aatcgatgag gatgagcctg
     8341 agatcgcagc ggtgcaccat cgttatctgg acggatgggt tcggtatcgg gcctacagag
     8401 tgccggacgc ggacttcatg gacaaagacc gttcagatcg tgggctggcc gactttgaag
     8461 actattttgg ccggcggcca actgcagacc atcggcggga caacaacgcg gatcggccgc
     8521 acagggtaaa ggcgtggtga tgaatggaat gacatctgat cctcgggatc atgaagcgtt
     8581 cgttcgagaa atcgacttgg gggcttatga gcctgaataa gctgaagctt ccgtcaatgc
     8641 gcctcggggc ctcagcggac attcagattc gcattgagac ggatggcaat gtctttgctc
     8701 cgatcgccac aattgatcgg tctgctccag cgaggatcgt gacgcaatca gcgcacggct
     8761 tactggatgg
//
LOCUS       DAOQEW010000160         8688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2724_length_8688_cov_8.003591, whole genome shotgun sequence.
ACCESSION   DAOQEW010000160 DAOQEW010000000
VERSION     DAOQEW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_8688_cov_8.003591"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..2051
                     /gene="flhA"
                     /locus_tag="PLS35_14980"
     CDS             3..2051
                     /gene="flhA"
                     /locus_tag="PLS35_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNI20025.1"
                     /translation="MSVGVVGILMVMLVPLPRFFLDLLLSFNITLSIIILLVGMQVRR
                     PLEFSVFPSILLMVTLLRLALNIASTRLILLHGNEGAAAAGEVIRAFGNFVVGGNYTV
                     GLVVFTILVIINFVVITKGAGRVAEVAARFTLDAMPGKQMAIDADLNAGLINDKEARQ
                     RRKEIAQEADFYGAMDGSSKFVRGDAVAAVVITLVNILGGLTIGVLQQGMTVAAAAQT
                     YTLLTVGEGLVAQVPALIVSTAAGIVITRAASEVNLGFEITRQVLISPKAIGTASGIL
                     LAMGLVPGLPHLAFLALGSLTGWMAFQLNEHQKTAVATPEPESPQVKAEEAATQVVPL
                     DLMEVQVGYGLIGLVDGGQGGALLDRIKGLRRQFAEQMGFVLPPIHIRDNLQLRPNEY
                     AALLKGVELAKSEVMPAHVLAIDPGTAQRGIIHGLPTKEPAFGLPALWVPEQQREQAQ
                     MAGYTVVDPGSAIATHLSELIKRHAHELLGRQEVQGLLDQLGKNHPKLVEEVVPNLIP
                     LGTLVRVLSHLLREGVPIRDLRTILETLADHAASTKDADILTEVARQALGRTITKQYL
                     APDGSLPIIGLDPRLDRTLADQANAVGQGNQWIPDPMVAQKLLSALKQAAERMVGRGH
                     QPVILCSPSLRRHLRKLTDRILHSVPILGLNEVDSVTRLQAMETVRLDLDGGDTRSLA
                     "
     gene            2048..3331
                     /gene="flhF"
                     /locus_tag="PLS35_14985"
     CDS             2048..3331
                     /gene="flhF"
                     /locus_tag="PLS35_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNI20026.1"
                     /translation="MKVRTFHVASMHEAIRSIKDTLGPDAVILSTKRVRSWDNGFGLL
                     GGSMLEVMAAIEDDAAVPESAPILGEDERSAPAVAQAPAVPAEESRFQKTLQGAIEKR
                     EDQPRPASRQASFVPQELSRTAKSSPVTKDGQGTTFQSFQRVYEDLLAQGVEHATAEA
                     CLRELRETLVEQGTSQRNSSLQLLRTVLLDRVSTAGPLLSNAGEQKIAMFVGPSGVGK
                     TSTIAKLATHYGIVEQRSVALITMDTYRPAAVEHLRLYAEVLGIELAVASSCEDARAA
                     IAQAREAELILIDTAGFNPYEPIAAQRWRGLLNGALPLEVHLVLAAGTRVPDIVVSTS
                     QCVDVPSLRLLFTKLDETTGYGGIFEATHRTGIPLSYWGTGQRVPDDLVQAQVNRLGD
                     LLLGGQVRTPGEQNGQPWIRQAAPAVVPGIKTYTR"
     gene            3359..4225
                     /locus_tag="PLS35_14990"
     CDS             3359..4225
                     /locus_tag="PLS35_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNI20027.1"
                     /translation="MAIEPDILDPQLNTFDGSVGPSRTQVITVTSGKGGVGKTNVVAN
                     TAIALAQTGKRVLVLDADLGLGNVDILLGLTPKYTLEHVLAKTCRLEEIALTGPNGIV
                     VLPASTGISQLTVLTEAQQLILQEELERLASTMDVLLIDTGAGISSTVTYFAAAAQSI
                     VVVVSPEPTSLTDAYALMKVLLRQYRERRFHVLVNMVKSPRDAARIYRKLEVAVSRFL
                     HISLDYLGAIPQDDYVPMAVSQQRAVIDLFPHAPASRAFRELTEAVAQLTPPALPKGT
                     VQFLWQQLLRTH"
     gene            4245..5006
                     /locus_tag="PLS35_14995"
     CDS             4245..5006
                     /locus_tag="PLS35_14995"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNI20028.1"
                     /translation="MMEARKMSATGNASRRVIAEADRERVIQEFTHVVKAMAHRLAFR
                     LPAYMDAEDLMSVGIMGLMDAMDKYDPTREAKFKTYAEFRIRGAMLDEIRSMDWIPRS
                     VHERISLLQKTYSELLHRFGRPPTDQEVGKELKMSADELDEFLTRSRGAVVISLDDLG
                     VQDADGHKIISMLTDSNQPDPLSVLVNERARHVLEAAIQDLPEKERLVLSLYYFEELT
                     MKEIGQALKVTESRVCQIHSKAILHLKARLEPVDV"
     gene            5167..6036
                     /locus_tag="PLS35_15000"
     CDS             5167..6036
                     /locus_tag="PLS35_15000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNI20029.1"
                     /translation="MSVPPSVTGESSAAVEPEPDHHHAWSDNDALTRSYDAARFGVLA
                     CLLFMAGGLYWTAAIGLLTISALTAYPVMVSIGVAFTLMIFGATFWTPRSRSSAVRPL
                     WSAAESGSKTTYDPVTGLPLNRLFLSLLNQALIRGQKHGRQVAVLIIELDYFTPAAES
                     PAELNRHLMYRIEAARVKSALRTTDTVARIAERTFAVLVDQITDADNLLLLARKMQGA
                     IALPITLDHHEVFLTGRIGLALSKRDDLDPNALLEAAARALEMARTDASGMSGIETAL
                     PQPQTDSTSTIAA"
     gene            6315..7082
                     /gene="flgF"
                     /locus_tag="PLS35_15005"
     CDS             6315..7082
                     /gene="flgF"
                     /locus_tag="PLS35_15005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNI20030.1"
                     /translation="MNRAIYPILSGAVAQEKQLQVFANNLANVNTAGFKQDQQGFRGL
                     LARGGSVGMGAASSGITSTISTRPAGPTERVFTEANGMRTTFEPGRIRITGNPLDLAI
                     QTDGFFEVKTPDGVRYTRNGIFSLDGQRRLVTNLGHPVMGTKGEIKVPPGNIQINAEG
                     GIQVDGNAIGTIKVVEFPPNEMPQKFAEGLFTGGKPSVSKKPQLQSGHIEESNVNSLS
                     EMVKMIQGMRSYESAQKLIQTLDHMTDTAIQDLGRVQ"
     gene            7093..7884
                     /gene="flgG"
                     /locus_tag="PLS35_15010"
     CDS             7093..7884
                     /gene="flgG"
                     /locus_tag="PLS35_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNI20031.1"
                     /translation="MIRAMWTAATGMTAQQLNVDTIANNLANVNTNAFKRSRAEFGDL
                     LYQIQRLPGTNASNVGVFPVGVQVGGGVRPITVAKEWMQGNMRQTSNDLDLAIDGAGF
                     FQVARPDGTIMYTRNGSFKRDNVGNLVTGDGDQLTPVITIPSGALKIDIGQDGTVSVL
                     LPGVTQASQVGQIQLVRFDNPSGLVAMGGNLFLDSFASGPAQQGTGGFSTGFGTLQQG
                     FLESSNVNLAEEMVNMIIAQRSYEINSKTIQASDEMMQIANNLRR"
     gene            7942..>8688
                     /gene="flgA"
                     /locus_tag="PLS35_15015"
     CDS             7942..>8688
                     /gene="flgA"
                     /locus_tag="PLS35_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNI20032.1"
                     /translation="MATISLLASAVLPAMAGERAPIRPLFRVHEALVQGAHSLPLARG
                     KHTIYPEQIRGVIRDFVKRELAGRAVDCQVALGDPQQPIVVPSGTMDLQVSANHSEEP
                     LGRRVFQIHLTVNGRFIKTVEATADVAAIVEVVVPVRSIKVDEEIAADDVATERLVLY
                     DLKQPFAMNPSDVVGKAAIRPLPPQNAIRLTSLRRPFAVHKGDRVTIEARQGGLSIQT
                     VGVTKSHGELGQTITVSNVDSGKDLRATVVG"
BASE COUNT         1774 a         2594 c         2565 g         1755 t
ORIGIN      
        1 tgatgtccgt gggcgtggtc ggcattctga tggtgatgct cgtcccgctg ccgcgcttct
       61 ttctggactt actgctgagc ttcaatatca ccctgtcgat catcattctc ctggtcggca
      121 tgcaggtccg gcgtccgctg gagttttcgg tcttcccctc cattcttctg atggtcacct
      181 tgctgcggct ggcgctcaac atcgcatcga cccgcctgat tctgttgcat ggcaatgaag
      241 gcgcggccgc ggcgggcgaa gtcattcgcg cattcgggaa tttcgtcgtc ggcggaaact
      301 ataccgtcgg tctggtcgtc tttacgattt tggtgatcat caactttgtc gtcatcacca
      361 aaggtgcggg acgcgtggcg gaggtcgccg cacggtttac cttggatgcc atgcccggca
      421 aacaaatggc catcgatgcc gatctgaatg ccggcctcat caatgacaag gaagcccgcc
      481 agcggcgaaa ggaaattgcc caggaagcag acttttacgg agccatggac ggttcgagca
      541 agttcgtccg tggcgatgcc gtggcggcag tggtcattac gctcgtgaat attttgggtg
      601 gcttgacgat cggcgtgctg caacagggaa tgacggtagc ggcggccgca cagacgtaca
      661 ccttgctgac ggtcggcgaa ggcctcgtcg cgcaagtgcc ggcgctcatc gtctccaccg
      721 ccgccggtat cgtcatcacc cgcgcagcct ccgaggtcaa cctcggattc gaaatcactc
      781 gccaggtcct gatctcgccg aaagccatcg ggacagcttc cgggattctc ctcgccatgg
      841 gattggtgcc gggattaccc catctggcct ttctggcgct cggtagtttg accggctgga
      901 tggccttcca actcaatgag caccagaaga cggcggtcgc aactcccgaa ccggaatccc
      961 ctcaggtcaa agccgaggaa gccgctaccc aggtcgtgcc gctggacctc atggaagtcc
     1021 aggtcggata cggactcatc ggtctggtcg atggagggca gggtggcgcc ttgctcgacc
     1081 ggatcaaagg cttacgacgt caatttgccg aacaaatggg attcgttctg ccgccgatcc
     1141 acattcgaga caatctgcaa ctcaggccca acgaatatgc cgcactcctc aagggcgttg
     1201 agctggctaa gtcggaggtc atgccggccc atgtgttggc tatcgatccg gggaccgctc
     1261 aacgtggaat cattcatggg ttgccgacca aggaacctgc gttcggactt ccagccctct
     1321 gggttcccga gcaacaacgt gaacaggcgc agatggccgg atacacggtc gtggatcccg
     1381 ggtccgccat cgcgacacat ctgtcggagc tcatcaagcg acatgcgcat gagttgctgg
     1441 ggcggcagga agtgcagggc ttattggatc agctaggcaa gaatcaccct aagctggtcg
     1501 aggaagtggt tccgaacttg attcctctcg ggacgctggt acgcgtgttg tcgcatctcc
     1561 tgcgagaagg cgtgcccatt cgcgacttgc gaacgatcct ggaaaccctc gccgaccatg
     1621 cggcgagcac aaaagacgcg gatattctca cggaagtcgc gcgtcaggcc ctgggacgga
     1681 cgattacgaa acaatatctc gcgccggacg gctcgttgcc gatcatcggc ctcgacccgc
     1741 gcctggatcg cacccttgcc gatcaggcca atgcagtcgg gcagggaaat caatggatcc
     1801 ccgatcctat ggtggcacag aaactgctca gtgcattgaa gcaggccgcg gagcgcatgg
     1861 tcggaagagg acatcaaccg gtgattcttt gttccccttc actacgtagg catctgcgga
     1921 aattgacgga ccgcattttg cactcggtac cgatcctggg attgaacgaa gtggatagcg
     1981 tgacgcgatt acaggctatg gaaacagttc gtctcgatct cgacggcggt gacacgagga
     2041 gcttggcatg aaggtacgga cctttcacgt tgcatcaatg catgaggcca ttcgctccat
     2101 caaggatacg ctggggccgg atgccgtcat tttgtcgacg aagcgcgtgc gatcctggga
     2161 taacgggttc ggcctcctgg gcggatcgat gctcgaagtg atggcggcga ttgaagacga
     2221 tgccgcggtg ccggaatctg cacccatact gggcgaagat gaacgttccg ctccggctgt
     2281 cgcacaagct cccgcggtgc cggccgagga gtcacggttc cagaaaacac ttcaaggcgc
     2341 catagagaag agagaggacc agccgcgccc tgcttcccgc caggcctctt ttgttcctca
     2401 agaactttcc cgcacggcaa agtcttcccc cgtcaccaag gacggtcagg gcacaacctt
     2461 ccaatcattc caacgggtct atgaagacct cctggcccaa ggcgtggagc atgccactgc
     2521 tgaagcctgt ttgcgagagt tgcgcgagac gttggttgaa caggggacgt cccagcgcaa
     2581 ttcttccctc cagcttttgc ggacggtact gctcgatcgt gtctcgaccg ccggtccgtt
     2641 gctgagcaac gccggcgagc aaaagatcgc gatgtttgtc gggcccagcg gcgtcggcaa
     2701 aacttcaacg atcgcgaagc tggcgacgca ctacggcatc gttgaacagc ggagtgtcgc
     2761 cctcatcacc atggacacct atcgtcccgc ggcggtggaa cacctccggc tgtacgccga
     2821 ggtcctggga atcgagctgg cggtcgcatc gtcatgcgag gacgctcgcg ccgctatcgc
     2881 gcaagcgcgt gaagcagagt tgatcctgat cgatacggcg ggattcaacc cctatgaacc
     2941 gatcgcggcg cagcggtggc gagggttgct gaacggggcc cttcccctgg aagttcatct
     3001 cgtgttggcc gccggcacgc gtgtgccgga tatcgtggtc agtaccagcc agtgtgtgga
     3061 cgtgccctcg ttgcgactgc tgtttaccaa gctggatgaa acgaccgggt acggcggaat
     3121 ttttgaagcc acgcatcgga cgggcatccc gctgtcttat tggggcaccg gtcaacgtgt
     3181 gcccgacgac ttagtccagg cgcaagtcaa ccgcttgggc gacctgctgc tgggtgggca
     3241 ggttcggacg ccgggagagc agaatggcca gccatggatc aggcaggccg caccggcagt
     3301 tgtgccaggc atcaagacct acacgcgata gacggtagcg aataacaggg gagagacgat
     3361 ggcgatcgaa ccagacattt tggatccgca actgaacacg tttgacggca gtgtcggtcc
     3421 ctcccgcacg caggtcatca ccgtcacgag cggaaagggc ggggtaggga agaccaatgt
     3481 ggtggccaac accgccatcg ccctcgccca gaccgggaaa cgcgtgctgg tgttagatgc
     3541 cgatcttggc ttggggaatg tcgatattct cctggggctg acgcccaaat acacgctgga
     3601 acatgtcctg gcgaaaacgt gccggctcga ggagatcgcc ttgaccggtc ccaacgggat
     3661 cgtggtgctt cctgccagca ccggcatttc gcaactgact gtcttgaccg aagcgcagca
     3721 gcttattctg caggaagagc tggaacgcct ggcctccacg atggacgtgc tgttgatcga
     3781 taccggcgcc ggcatctcgt caacggtgac gtacttcgcg gctgctgccc agtccatcgt
     3841 ggtcgtcgtg tctccggagc ccacgtcgct gacggacgcc tatgcgttga tgaaggtctt
     3901 gctgcggcaa taccgtgaac ggcgctttca cgtcctcgtc aatatggtga agtcaccgcg
     3961 tgacgccgca cgcatctacc gtaagctgga agtggccgtc agccgctttc tccacatttc
     4021 tctcgactac ctgggcgcca tcccgcagga cgactatgtg ccgatggcgg tgtcccagca
     4081 gcgtgccgtc atcgatctgt ttcctcacgc gccggccagc cgcgcattcc gagagctgac
     4141 tgaagcggtg gcccagttga cgcctccggc gcttccgaag ggcacagtgc agttcctctg
     4201 gcaacaactc ttgcggacgc actgactgaa aggactgaac tcgcatgatg gaagcacgaa
     4261 aaatgtctgc gaccggaaat gcctcgcgcc gggtgattgc ggaagcggat cgtgagcggg
     4321 tcattcagga attcacgcat gtcgtgaagg ccatggccca tcggttggct ttccggctgc
     4381 cggcctacat ggatgcggag gacctgatgt ccgtgggcat catggggctc atggatgcga
     4441 tggacaaata cgatccgacc cgcgaggcca agttcaaaac ctatgcggaa ttccggattc
     4501 ggggcgccat gcttgatgaa atccgatcga tggattggat tccgcgctcg gttcatgaac
     4561 gcatttccct gcttcaaaaa acctactccg agctgttgca tcgattcggt cgtccgccaa
     4621 cagaccagga agtcggcaaa gaactgaaaa tgtctgcgga cgagttggac gagttcctga
     4681 cccgctcgcg aggcgccgtg gtcatcagcc tggatgacct cggagtccag gatgcggatg
     4741 gacacaagat catcagcatg ttgaccgact ccaatcagcc ggatccgctc tcagtgctgg
     4801 tgaatgagcg agcccgccac gtgctggaag ccgcgatcca ggacttgcct gaaaaggagc
     4861 gcctggtgct gtcactctac tatttcgagg agttgaccat gaaagagatc gggcaggcct
     4921 taaaagtcac ggaatcgcgt gtctgccaaa tccattcgaa agccattctt catcttaagg
     4981 cccggcttga accggttgat gtctagatgt caaaccggcc attcgcatcc actgctcctc
     5041 cttgttgtcg gttctatgaa cgatcttcag tgacaccgac ccaccgcctt tagtttcccc
     5101 ccgctccgtc cgatacgctc taaaccccaa tagccattgg gcaacctatc agttctcttc
     5161 gcgcgtatga gcgtgcctcc atcagtaacc ggcgagtcga gcgctgccgt tgagcctgag
     5221 cccgaccatc atcatgcctg gtcggacaat gacgcgctca cccgatccta tgatgccgcc
     5281 cgattcggcg tattggcctg tctcctgttt atggcaggcg gcttgtattg gaccgcggcc
     5341 attggtctcc tgaccatctc cgcattaacg gcctatcccg tgatggtttc gatcggcgtc
     5401 gccttcacgc tgatgatttt cggggcgacc ttctggactc cgcgaagtcg ctcttccgct
     5461 gtgcggccgc tctggagcgc cgcagaaagt ggcagcaaga ccacgtacga tccggtcacc
     5521 ggcctccccc tgaatcgtct gtttctctcg ctcctgaatc aagccctgat ccgtggacag
     5581 aagcatggac gccaggtggc ggtcctcatc atcgagctgg attatttcac gcccgccgcc
     5641 gaaagccctg cagaactgaa ccgtcatctc atgtaccgga tcgaagccgc ccgcgtgaaa
     5701 agcgccttgc gcacgaccga cactgtggcg cgaatagcgg aacggacgtt tgccgtgtta
     5761 gtggatcaga tcaccgatgc ggataacctg ttgctgctcg ccaggaaaat gcaaggcgcc
     5821 atcgccttgc cgattaccct cgatcatcat gaagtgttcc tcaccggccg gattggtctc
     5881 gcgctttcca agcgggacga tcttgacccc aatgccttgc ttgaggctgc tgcacgagcc
     5941 cttgagatgg cgcggaccga cgcgtcggga atgtccggca tcgagacggc gctccctcag
     6001 ccacagacgg attccacctc cacgattgcg gcctaattct ccctcactca tcctcggcac
     6061 ctgctcaggt aggcaggact ttcccagcct gcggtaaaga cttccactcg ccagcatctt
     6121 tgacagctct cgtcgaccgc accgggctgc gcctgcgcgc gtttacgcag cacaccgtcg
     6181 ccatctatcg gcaatcatga gtgattttct cagtctgcgg tagcggtgcg agcgatgcag
     6241 cgcttcccgc agcaatggca tgtcgattgc taactctccc tgtgaacaga gagaagggtt
     6301 ggaaccgaca ccatatgaat cgagctatct atcccatact ttccggcgca gtcgcccaag
     6361 aaaaacagct ccaggtattc gccaacaatt tggcgaacgt caatacagcc ggattcaaac
     6421 aggaccaaca ggggtttcgc ggcctcctgg cccgtggcgg gtcggtgggt atgggcgccg
     6481 catccagcgg catcacctcg acgatatcaa ccagaccggc cggccccacc gagcgcgtgt
     6541 ttaccgaagc aaacggcatg cgtacgacct tcgagccggg acggatccgc atcaccggca
     6601 atccgctcga cctggccatt cagaccgacg gattttttga ggtcaagacg cctgacggcg
     6661 tgcgctatac ccgcaacgga atattctccc tcgatggcca acgacgtttg gtcaccaatc
     6721 tgggacatcc ggtcatgggc acgaaagggg agatcaaagt gccgcccggc aacattcaga
     6781 tcaacgccga gggcggcatc caagtcgatg gcaacgcgat cggcacgatc aaggtcgtcg
     6841 aatttccccc taacgagatg ccgcagaaat tcgcggaagg cttgttcacc ggcgggaagc
     6901 ccagcgtgtc gaagaaaccg cagctccagt ccggccatat cgaagaatcg aacgtcaatt
     6961 cgttgagcga aatggtgaag atgattcagg gcatgcggag ttatgaatcg gcgcagaagc
     7021 tcattcagac gctggaccat atgacggata cggccatcca ggatctcggc cgggtgcaat
     7081 aggaggagtt gtatgattcg tgcaatgtgg acggccgcca ccggaatgac ggcccagcaa
     7141 ctcaacgtcg acacgatcgc caacaacctg gccaacgtga acacgaatgc gttcaagcgc
     7201 agccgtgcgg aattcggcga cctgctgtat cagattcaac ggttgcccgg cacgaacgcg
     7261 tcgaacgtcg gcgtgtttcc cgtgggcgta caggtcggcg gcggcgtccg tcccattacg
     7321 gttgccaagg aatggatgca gggcaacatg cgacagacga gcaacgacct cgatctggcc
     7381 atcgacggcg caggattttt ccaagttgcg cgtccggacg gcacgatcat gtatacgcgt
     7441 aacggatcct tcaagcgcga taacgtcggc aatctggtca ccggcgacgg tgatcagttg
     7501 accccggtca ttaccatccc ttccggcgcg ctcaaaatcg atatcgggca ggacggcacc
     7561 gtctccgtct tgctccccgg tgtgacccag gcgtcgcagg tcggacagat tcagctcgtg
     7621 cgtttcgaca atccctccgg tcttgtcgca atgggcggca atttgttcct cgacagtttc
     7681 gcgtcgggac cggcgcagca gggaaccggc ggattttcca ccggcttcgg cacgttgcag
     7741 cagggctttc tggaaagttc gaacgtcaat ctggcggaag agatggtcaa catgatcatc
     7801 gcccagcgca gttacgagat taactcgaag acgatccaag cgtccgacga aatgatgcag
     7861 atcgcgaata acctgcgccg gtaatcggcg ccgtaccttg aaccaggggt gacacctgtg
     7921 aatcgagccg gccttatctc tgtggcaacc ataagcctgt tggccagcgc cgtccttcct
     7981 gcaatggcag gggagcgggc tccgattcgg cctctgttcc gggtgcatga agccctcgtg
     8041 cagggtgcgc acagtcttcc actggctcgc gggaaacaca cgatctatcc ggaacagatt
     8101 cgtggagtta tccgcgactt cgtcaagcgt gaacttgccg gacgcgcggt cgattgccag
     8161 gtggcgctgg gcgatcccca acagccgatc gtggtgcctt ccggcacgat ggatctgcaa
     8221 gtcagcgcga atcattccga ggagccgctc ggccggcgag tgttccagat tcacctgacc
     8281 gtcaacggcc gctttatcaa aaccgtcgaa gccacagcgg atgtcgctgc catcgtcgaa
     8341 gtggtagtcc cggttcgttc gattaaggtc gatgaggaaa tcgctgcgga cgacgtggcg
     8401 accgaacgtc tcgtgctgta cgacctgaag caaccgttcg ccatgaatcc gtcagacgtc
     8461 gtcggcaaag cggcgatccg cccgctgccg cctcaaaacg cgattcggct gacctccctc
     8521 cgtcgcccgt ttgcggtgca taagggcgat cgggtgacga tcgaagcccg gcagggggga
     8581 ctctccattc aaaccgtggg cgtgacgaag tcgcatggcg aactgggtca aaccatcacg
     8641 gtgtccaatg tcgattccgg caaggatcta cgagcgactg tggtcggt
//
LOCUS       DAOQEW010000161         8667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2736_length_8667_cov_9.144333, whole genome shotgun sequence.
ACCESSION   DAOQEW010000161 DAOQEW010000000
VERSION     DAOQEW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_8667_cov_9.144333"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            496..861
                     /locus_tag="PLS35_15020"
     CDS             496..861
                     /locus_tag="PLS35_15020"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI20033.1"
                     /translation="MQNRYSQRVPMDCSVMFAGDNCVGEGRILDISLPGCLLESPKQM
                     TAGEYLQLRLFLPDHQAPLHVPLAAVRWVEGSKLGIEFIRTSEDEQRRLEQFVRRIPA
                     AAHPSICREGIVLLGATEQ"
     gene            complement(1246..2661)
                     /locus_tag="PLS35_15025"
     CDS             complement(1246..2661)
                     /locus_tag="PLS35_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI20034.1"
                     /translation="MEQERILVVDDDEGLLHLLRMRLSALGFSVTPCTTGRDALVAAR
                     QETFDIAITDLRLRAEDGLVLTEELLQVQPGLPVIILTAHGSIPNAVEAMQRGAFGYL
                     TKPFDDKELKATIDKALIQLRMTREIQRLKSLVKELYGLENVIARSPAMQRLFQQVAQ
                     IADSDATILLTGETGTGKEVLARVLHANSRRAKGPFVALNCAAISEHLFESELFGHIR
                     GAFTNAMAAKRGLFQSANGGTLFLDEIAEMSLPMQVKLLRAVQEREVREVGADYTTKV
                     DVRIITATNKDLAECVKQGTFRHDLYYRISVVPLNIPALRERRDDIPLLAQHFLKQSA
                     RRSNKDVRGFTPAAMHRLIGYPWPGNVRELENAVEKAVVMSRQDMVLPELLPSAGAST
                     DIGLKPLTEAKEEFERSYLRNVLQMTGGNISRAAQFAGRYRADFYKMLKKYGLHPSML
                     KERHDLDLAEIEESAEELKDV"
     gene            complement(2670..3320)
                     /locus_tag="PLS35_15030"
     CDS             complement(2670..3320)
                     /locus_tag="PLS35_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20035.1"
                     /translation="MLQAILRKDSSLVTKCASKNTCDHVFFTRALTSLYESRESAIHN
                     FEKVIAVAPKSQLAVSSRLWLQLLQAGEVPSDQSWLRSVWNGPAIAQTQGVLSQTIER
                     TVRDLLDRELTIQQLRALQDSEAQSLEAMQRELQDQERKAELFNSRRESHRMSVEPAT
                     LQSLQRQVSDRDKKIEELTSQLEALKRIDQEMRGKTRPIKPPSSVLPSQQSEPSKP"
     gene            complement(3440..4990)
                     /locus_tag="PLS35_15035"
     CDS             complement(3440..4990)
                     /locus_tag="PLS35_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNI20036.1"
                     /translation="MSEVVYGLTCTAASAGPRIHHQGADLVVRLSIFWRLVLTYLVII
                     AVMTGVNVYALLQIRTLAGLNTEVGSHHHPEIDSAKRLLTSIYEQVQGEKKYVAVPAA
                     TFLEHFDSESKEFQQVLQGLLAHDVSEPETRLLKEIEGLQQAHLSLFQSQLAESSGHP
                     EASAGYDARREAVVGKMAAALQQYIGLHERRLAVGVNESHASSVHAEAVTRNLVLVAL
                     LFGLGMAAVASYNILRPLRRLQATIQEMGQGNFKASWDGQAPRELRELGETVKWMGTK
                     LQQLDDIKSEFLAHVSHELRTPMASIQEGTHLLLDEIPGPLTQDQRTTLRIMSDSSRR
                     LMYLISTILDLSKMESGMMEYRFVPTDLRRVADISINKIRLLADAKHVQLVIEAPVER
                     HWVRADSTRIEQVLDNLLSNALKYSPEGAVVRLQMTPRRDEGLLFVDVADAGPGIPAE
                     ELPHIFERFYQGRTKSRQVSVGSGLGLALAKKVVEAHGGRIWVESEVGKGTTVRFILS
                     LMKPGMLA"
     gene            complement(5057..5875)
                     /locus_tag="PLS35_15040"
     CDS             complement(5057..5875)
                     /locus_tag="PLS35_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20037.1"
                     /translation="MLTIFNSTLQLYRDALHATWRSLVRGWFVILAVIVFTLLMMLAH
                     QIARPLGMLGGFLLGAVNALLIGATLSLIEQSIKGMRTITMHDVLESMGHYFWDVISV
                     GFVLWIPTMLLDTSLRANPDSQFLVMACLLLVFILLNPAPEVIYLTRHDSPLDVLKRS
                     YDFVLDNWIEWFLPMALVLAPLGLSLFFKLSSQLGRGAGLDFIQLVLVPFTILSAWLT
                     DLGISANLTGMMALLLTPPLMVGMLLFRGHLFSALSSTSRRQRAYQSQFHDSAR"
     gene            6114..>8667
                     /gene="clpB"
                     /locus_tag="PLS35_15045"
     CDS             6114..>8667
                     /gene="clpB"
                     /locus_tag="PLS35_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNI20038.1"
                     /translation="MDMNRMTVKLQEALQSASSLAMRRGHQGIDVEHLLLALLEQERG
                     IASSLFEQAGVSPAAVRQAADQALAKVPQVQGPGAAPGQIHLTPRLGAVLTKAEDQMK
                     ELRDEFISVEHVVLAMVEEGGVFRRLNLTRDRLLTALQQVRGNQRVTSQDPEGTYQAL
                     EKYGRDLTKLAGQGKLDPVIGRDDEIRRTIQILSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKGDVPEGLKQKRVVVLDMGALVAGAKFRGEFEERLKAVLKEVQSAQGQVLLFI
                     DELHTVVGAGAAEGAMDAANLLKPMLARGEMHLIGATTLDEYRKHIEKDAALERRFQT
                     VLVDQPTVEDTISILRGLKERYEVHHGVRIKDAALVAAAKLSNRYIGDRFLPDKAIDL
                     VDESAARLRTEIDSLPAELDEVSRKVLQLEIEREALKKETDAGSKARLQSIEKELNEK
                     NRDLQALKTRWESEKNSVSKLRKIRQQIEEVKQNIERSERAYDLNKVAELRYGELPRL
                     ERELELEQQSLGKKQNETRLLKEEVDEEDIAAVVSRWTGIPVTRLVEGEMEKLLKLDE
                     LLHKRVVGQEQAVTAVADAVVRARSGIKDPNRPIGSFLFLGPTGVGKTELARALSVTL
                     FDDESNLIRIDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRHPFSVILFDEIE
                     KAHHDVFNILLQVLDDGRLTDSQGRTVDFKNTVLIMTSNIGSQHILEAQQAGASYETM
                     KNQVTGELRAHFRPEFLNRVDEIVVFHALGNEHLAKIVEIQLERLRARLMERRITLTV
                     TPEALQNLGRRGYDPVYGARPLKRLIQQEIETPMARQLIKGELRDGDTAVVDL"
BASE COUNT         2117 a         2392 c         2339 g         1819 t
ORIGIN      
        1 acaaatacct agaactatca gagggttgaa gggagggaat caaagtagga gccagttact
       61 acagtcctgc taccatattt gctgcatagg cgatccatct cctgccaatg gcaactattt
      121 ttctggtcgt ccgaccacca gttgaataca tctaacccac gctgacatgc cacgtagaaa
      181 gagattcttc tgtctgaaag ctgagtcaat gctgtggggt agcattgctc ctacaaaggc
      241 tttcaggtga aggcttcgac tggcagcgtc gaacggtgtt tgtcccgctt actgataaag
      301 atccaacctg gcaagaattc cctgaacccc gtaaattctg cagtttttag ccctcaggca
      361 ttcctgtcgc caagggtccg cagcgtcccc actatagtgt taccaattct gaggactagg
      421 cgtacgctgg ttcttagatt acagtcgcat tgaaagcgac agtataggtc ccgaggcata
      481 cgaggaggag gcaccatgca gaatcgatac agtcagcgag tgcctatgga ctgttcggtc
      541 atgtttgccg gagataattg tgtgggggaa ggccgcattt tagatatttc tcttccaggc
      601 tgtctccttg aaagtccgaa acaaatgact gcgggagagt acttgcaact aagattgttc
      661 ctgcctgatc accaagctcc gctgcatgta ccgctggctg cagtcagatg ggtagaaggt
      721 tctaagcttg gcatcgaatt tattcgaaca tctgaagatg aacagcgccg tcttgagcaa
      781 ttcgtccgga ggattcctgc tgctgcccat ccatcaatct gcagggaagg aatcgtccta
      841 ctcggggcaa cagagcagtg agtgcggctc gaacgagtgc atgacctgat cttgatagga
      901 gctcttcatg tcaaagcatt ttaggttgcg cacctaccag aggatcacac agtgcaatgt
      961 tggctactac ttatcagacg attttctcgg gaaggggata atcagggata tttcgagtgg
     1021 cggatggcgg ctgcaaggcg atcatcaagt caccataggt atgaaactta ccttacgaat
     1081 ggatttacct gagcacaatt cgccgttaga aatagaagag gcgtcagttc agtgggtgag
     1141 cgggaaagat ttcgggattc agattagaaa gatccgtcac gcaacggcaa gacgattaga
     1201 acgcttaata ggaaatcacc tctgcacgct ccccccactt gaacgctaaa cgtctttgag
     1261 ttcctcagca gactcctcaa tctcagcgag atccaggtca tggcgctcct tcaacattga
     1321 aggatgcagc ccatacttct ttaacatctt ataaaagtca gcacgatatc ggccggcaaa
     1381 ctgggccgct cgcgagatat ttccgcccgt catctgtaac acattgcgca aatacgaacg
     1441 ttcaaattct tcctttgctt cggtcagcgg cttcaaccca atatcggtcg acgcaccagc
     1501 cgagggaagt agttccggca ataccatgtc ctgccgtgac atgaccacgg ctttttccac
     1561 ggcattttca agctccctga cgttcccagg ccatggatat ccaatgagcc gatgcatggc
     1621 cgctggtgta aatccacgaa catccttgtt agaacgcctc gcactctgct tcaaaaaatg
     1681 ttgggccaag agaggaatgt cgtcccttcg ttcccgcaag gcaggaatat tcagtggaac
     1741 aacggaaatt cggtaatata agtcgtgtcg gaacgtcccc tgcttcacac attccgccaa
     1801 atccttgttc gtagccgtaa tgatgcgcac atccactttt gtcgtgtaat ccgcaccgac
     1861 ctcacgcact tcccgctcct gcacagcacg caacagtttc acctgcatgg gcagagacat
     1921 ttccgcaatt tcgtcgagga aaagcgtccc tccatttgcg ctttgaaaaa gaccacgctt
     1981 cgccgccatg gcattcgtaa acgctccacg gatatgcccg aacagttcgc tttcaaaaag
     2041 atgttcgctg atcgcggcgc agtttaatgc gacaaacggg cctttggctc gacggctatt
     2101 cgcatggagg actcttgcca ggacttcttt ccctgtccca gtctcgccgg tcaacagaat
     2161 ggttgcatct gaatcggcga tttgagcgac ctgttgaaac aatcgctgca tggcgggact
     2221 tcttgcgatg acattttcaa ggccataaag ctccttgacc agggacttca gccgctggat
     2281 ctcccgcgtc atccgcaact gaatcagggc cttatcgatc gttgctttca attccttgtc
     2341 atcaaaaggc ttggtcagat acccaaaggc cccccgctgc atcgcttcta cggcgttggg
     2401 gatactcccg tgcgcggtca gaatgatgac gggaagtccg ggctgaacct ggagaagttc
     2461 ttccgtgagg accaacccat cctctgcgcg caaacgcaga tcagtgatcg cgatatcgaa
     2521 cgtttcctgt cgcgcagcaa cgagcgcatc gcggcctgtc gtgcaggggg tcaccgaaaa
     2581 tccaagcgca gataatcgca tccgcaacaa atgcaacaga ccttcatcat catccacaac
     2641 gagaattcgt tcttgttcca tgacggtcct tacggtttgg acggctccga ttgttgcgat
     2701 gggaggacac tcgagggagg cttgatcgga cgggttttgc ctctcatttc ctgatcgatg
     2761 cgcttgagtg cttcgagctg gctcgtgagt tcttcgattt tcttgtcgcg gtcgctgact
     2821 tgccgttgca aactctgaag cgtcgccggt tcaaccgaca tgcgatggga ctcgcgcctg
     2881 gaattgaaca gctccgcttt gcgctcctga tcttgaagct cgcgctgcat ggcctcaagg
     2941 gattgagctt cgctgtcctg taaggcacgt aattgttgga tggtaagttc gcggtcgaga
     3001 agatcccgca ccgtgcgttc aattgtttga gagaggaccc cctgcgtctg agcaatcgca
     3061 gggccattcc agacggaacg cagccaagac tgatcggaag gcacttcgcc ggcctgtagc
     3121 aattgtaacc agagtcggct ggacacagcc aactggctct tgggggcgac ggcaatgact
     3181 ttctcgaagt tatggatggc cgactctcga ctttcgtaca gagacgtcaa tgcgcgggtg
     3241 aagaatacat gatcacaggt atttttacta gcgcatttcg tgactaacga ggaatcttta
     3301 cgtaaaatgg cttgcaacat tttgccatct cccccatcgt ttgagaagta tggcgtctga
     3361 gctgggggcg aagggagggt gccacaggcg gacacaagtc ctacccacag aaccatccct
     3421 atggggcgac accattgcac taggccaaca tccccggttt cattaacgat aaaatgaatc
     3481 gcacggtcgt cccttttccc acttcacttt caacccaaat ccttccgccg tgagcctcta
     3541 cgactttttt cgccagggct aacccgaggc cgctcccaac cgaaacctgc cgactcttcg
     3601 tgcgtccctg ataaaaccgc tcgaaaatat gtggcaattc ctccgccggt atacccggtc
     3661 ccgcgtctgc cacatcgacg aagagcaggc cttcatcgcg ccggggagtc atctgcaacc
     3721 tgacaaccgc accctcgggg ctgtacttca acgcattcga gagcagattg tccagtactt
     3781 gttcgatccg cgtactgtct gccctgaccc agtgccgttc caccggagcc tcaattacca
     3841 gctgtacatg ttttgcatct gccaacaatc ggattttatt gattgaaata tctgcgaccc
     3901 gccggagatc cgtcggcaca aaccgatact ccatcatgcc ggattccatt ttggatagat
     3961 ccaggatggt cgagatgaga tacatcagcc gccggctact gtcggacatg atgcgcagcg
     4021 ttgttcgctg atcctgggtc aatggaccgg gaatttcgtc caagaggaga tgggtgccct
     4081 cctgaatcga agccattggg gtacgcaatt catgcgagac atgagcgaga aattcgcttt
     4141 tgatatcatc cagttgttgc aacttggtgc ccatccactt gaccgtttca cccaattccc
     4201 gcaattcgcg aggggcctgc ccatcccatg aggccttgaa attcccctgc cccatctcct
     4261 gaatggtcgc ctgcagtcga cgcaacggac gaagaatgtt gtaactggcc actgcggcca
     4321 tgccgagacc gaagagcagg gccaccagca ctaaattgcg cgtgacggct tcggcatgca
     4381 ccgagctggc gtgcgattcg ttgacgccca cggcaaggcg ccgctcgtga aggccgatgt
     4441 attgctgaag cgctgcggcc attttcccaa ccaccgcttc gcgccgcgcg tcgtaaccgg
     4501 cactcgcctc cggatggccg ctcgactcag ccaattgcga ttgaaatagt gacaaatgcg
     4561 cttgctgaag cccctcgatc tccttcagca gcctcgtctc gggctctgag acatcgtgag
     4621 cgagcaaccc ttgtaagact tgttgaaact ctttgctctc actatcgaaa tgctcaagaa
     4681 atgtggcggc aggcacagcc acatatttct tctcgccctg aacctgttca tagatcgacg
     4741 tcagcaagcg cttcgccgaa tcaatctccg gatggtggtg ggacccgact tccgtattga
     4801 ggccagccag tgtccgaatc tgcaacaacg catagacatt gacgccggtc atcaccgcga
     4861 taatgaccag atacgtcaga actaaccgcc agaaaatcga gaggcgcacg accaggtccg
     4921 ctccctggtg atggatgcga ggccctgcag aagcagcagt gcaggttaag ccatacacca
     4981 cttcactcat cccctcaaca agttatccat attatgcaga aacaggacgc cgtcctggtc
     5041 tggctggaaa gacctcctag cgagctgagt cgtgaaactg cgattgatac gcgcgttgtc
     5101 gacgagacgt ggaagagagc gccgagaaaa gatggccacg aaacaggagc atgcccacca
     5161 tgagcggtgg agtcagcagc agcgccatca ttccggtgag gttggcggaa atcccgaggt
     5221 cggtaagcca ggcgctgaga atggtaaagg gcactaagac caactgaatg aaatctaacc
     5281 ccgctccccg ccccaactga ctcgacagtt taaagaagag ggacaatccg agtggcgcca
     5341 aaaccagggc catcggcagg aaccattcga tccaattatc caacacgaaa tcataactgc
     5401 gcttcaagac atccagtgga gaatcatgcc tggtgagata gatcacctcc ggagcgggat
     5461 tcagcaaaat gaagaccagt aataaacaag ccattaccag aaattgactg tcggggttcg
     5521 cgcgcagact cgtgtccagg agcatggtgg gaatccagag cacaaacccg acgctgatga
     5581 cgtcccaaaa ataatgcccc atactttcca gtacgtcatg catggtgata gttcgcatgc
     5641 ccttgatgga ctgctcgatc aggctgagcg tcgcaccgat caacagcgca ttcacggcac
     5701 ccagcagaaa tcccccgagc atacccaggg gacgagcaat ttgatgggcc aacatcatca
     5761 gcagcgtgaa cacgatgacg gcgagaatca cgaaccatcc gcgaaccagg gagcgccagg
     5821 ttgcgtgaag cgcgtctcga tagagttgga gcgtcgaatt gaatatggtc aacatggatc
     5881 agtcaatgca ggttctcgaa cagagagaag cggctcttag cggaaagcga gctccccacg
     5941 tcgggcggac aataagatgg attcccctga gaggtcaagt cacacccggc aagattccac
     6001 gcagtcgcca gacagcaccg caagaaatag cgggtatcgg ctggtattga cttgcccagc
     6061 gatatgatta tgttctctca gtagatagac acatccaccc aggagtcagc cacatggata
     6121 tgaaccggat gactgtcaag ctacaagagg ccctgcaaag cgcatcatcc cttgccatgc
     6181 gccgtggcca tcagggtatc gatgtcgagc acctcctgct ggcgcttctg gagcaagagc
     6241 gagggattgc gagctctctg ttcgaacagg ctggtgtctc gccggccgcc gtgcgccagg
     6301 cggcggatca ggcactagcg aaagtgcctc aagttcaagg gcctggtgct gctcccggac
     6361 aaatccatct cacaccgcgg ctcggtgcag tgctgaccaa agccgaagac cagatgaagg
     6421 aattgcgtga cgagttcatc agcgttgaac acgttgtgct ggccatggtc gaggaaggcg
     6481 gcgtattccg ccgcctcaat ctgacacgag accgtctcct cacggcgttg cagcaggtcc
     6541 gtggcaatca acgggtgaca agccaggacc cggaaggcac ctaccaggcg ttagagaaat
     6601 atgggcgaga tctcactaaa ctcgcgggcc agggcaagct cgatccggtc atcggccgtg
     6661 atgacgaaat ccgacgtacc attcaaattc tgtcgcgacg aaccaagaac aatcccgtcc
     6721 tgatcggtga gccgggtgtc ggtaaaacag ccatcgtcga aggcttggcg caacgcatcg
     6781 tcaaaggcga tgtgcccgaa gggctcaaac agaagcgtgt cgtcgtgctt gatatgggcg
     6841 cgctcgtcgc cggggcgaaa ttccgtggag aattcgaaga gcgcttgaag gccgtcctca
     6901 aggaagtcca atcggcgcag gggcaggtgc tcctcttcat cgacgaactc cacaccgtgg
     6961 tcggtgcagg tgcagccgaa ggggcgatgg atgcggcgaa cctcctcaag ccgatgctgg
     7021 ccagaggcga aatgcacctc atcggtgcga ccacgctgga tgaatatcgc aaacacattg
     7081 agaaagacgc cgccctggag cgacggttcc aaactgtgct cgtggatcag ccaacggtcg
     7141 aggataccat ctccattttg cgcgggctca aagaacgcta cgaagtgcat catggcgtgc
     7201 gaatcaaaga tgccgccctc gtcgcggcag ccaagctgtc aaaccggtac atcggcgatc
     7261 ggtttttgcc ggacaaggcg atcgatctcg tcgacgaatc cgccgcgcgg ctccgtacag
     7321 aaatcgacag tttgcccgcg gaacttgatg aagtctcccg gaaagtcttg caactggaga
     7381 tcgagcggga agccctcaaa aaagaaaccg atgcaggcag caaggcgcgt ctccaatcga
     7441 ttgaaaaaga actgaacgaa aagaatcgcg acctgcaggc ccttaagact cgatgggaat
     7501 ccgaaaaaaa ttcggtgagc aagctgcgga agattcgcca gcagatagaa gaggtgaaac
     7561 agaacatcga acgctccgaa cgcgcctacg atttgaacaa agtggcggaa ctgcggtacg
     7621 gcgaattgcc caggctggag cgggaacttg aactcgagca gcaatcgctt ggcaaaaagc
     7681 aaaacgaaac ccgtctgctg aaggaagagg tggacgaaga agatatcgcc gccgtcgtca
     7741 gtcgttggac cgggattcca gtgactcgtc tcgtcgaggg agaaatggaa aaacttctca
     7801 agctggacga actgctccac aagcgtgtcg tgggacagga acaggccgtc acagccgtgg
     7861 ccgatgcggt cgtgcgagcc cgctccggca tcaaagaccc caaccggccc atcggttcct
     7921 tcctgtttct cggaccgacc ggggtgggca agacggaatt ggcccgcgcc ctctctgtca
     7981 ccctattcga tgacgaatcg aacctgatcc ggatcgacat gtcggaatat atggagaagc
     8041 acacggttgc caggttgatc ggcgcgcctc ccggctatgt gggctatgag gaaggcgggc
     8101 aactgactga agcggtgcgc cggcacccgt tctccgtgat cctcttcgat gagatcgaaa
     8161 aagcgcatca cgatgtcttc aatattctgc tgcaggtgct cgacgatggc cggctcaccg
     8221 attcgcaggg gcgaaccgtg gatttcaaaa acacggtgtt gatcatgacc tcgaatatcg
     8281 ggagtcagca catcctggaa gcgcaacaag ccggtgcctc ctatgagacg atgaaaaatc
     8341 aagtgaccgg ggaattgcgg gctcacttca ggccggaatt tctgaaccgg gtcgacgaga
     8401 tcgtcgtctt ccatgccctg ggcaacgaac acttggcgaa gatcgtggag attcaattgg
     8461 aacggttgcg agcccgtttg atggaacgcc gaatcaccct gacggtgacg ccggaagcgc
     8521 tacagaacct cggccgacgt gggtacgatc cggtgtatgg cgcgcggcca ctcaaacgac
     8581 tcattcagca ggaaattgag acgccgatgg cgagacagtt gatcaaggga gaactgcggg
     8641 acggagatac ggccgtggtg gatttga
//
LOCUS       DAOQEW010000162         8609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2763_length_8609_cov_10.547931, whole genome shotgun sequence.
ACCESSION   DAOQEW010000162 DAOQEW010000000
VERSION     DAOQEW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2763_length_8609_cov_10.547931"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1398)
                     /locus_tag="PLS35_15050"
     CDS             complement(79..1398)
                     /locus_tag="PLS35_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HNI20039.1"
                     /translation="MIELIKSALSMGWPALGLLIGLMFYFKASISDPVANKRAVFKTF
                     IGIWGALLLFMAIANYKMNFFGESRLLPVSLVLITSMTFMMALYFTNISALLKIGGFM
                     FFIAAALSGYGNWLPQVEGGFPPIEEKKDFSNMPQTELADEGEKIIFGGVGQNKVQGA
                     IGKGQCPLCHAFHKGMLGERAPNLDGIPERAETQINDPRYHKGNPAARDSNQKEAFAG
                     SGTAENGIQYIAESHACPSCFVVAGFGVKGTNDKESPMPSIHKPPISLSLPELAAVDT
                     WLYVREGREAPGFDEIVKSYEKFIPESDRPKPPTEGDKAAGGGGASLMADGTEPVDQI
                     FAKGQCVACHTIPGIAGATGTIGPKLVEGTNAPLRIKDKDYKGKAKSVPDYIMESIVE
                     PSAYVVKGFPDNTMPKVFGQKLSAGALKKLVDYLSQIQEGKEPPKAS"
     gene            complement(1484..3400)
                     /locus_tag="PLS35_15055"
     CDS             complement(1484..3400)
                     /locus_tag="PLS35_15055"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI20040.1"
                     /translation="MGSVTRTKLMSVMALCAMIGLLLMPILVAIPALADGGAPAADAK
                     KEGGEAKVEKGKDVYYKTEGIVSGPPAPKTTDSEKDYPRYNFESRVLLWFANQQHLYY
                     GSFVLAVPIFCMVIEFMGVVTKDKALAKRYDQLAYDFIKISLTAYSLTAILGGILIFT
                     FLTLYPAFFQYLSGIFRPVMHIYALMFVAESGTLYIYYYGWDKMKEGFLKWIHLSMSV
                     VLNVIGTILMFLANSWIGFMMSPAGVDEQGRYLGNIWHVIHTALWNPLNLHRILGNMA
                     FGGGVVAAYAAYRFLSAKTDEERAHYDWMGYIAMALGVAFLIPLPFAGYWLMREVYAY
                     RQQMGITLMGGLLAWLFIIQATMIGILFLSTNYYLWQALGRMRGAEKYQRYIKYLVFL
                     LTCGYLVFITPHTIVMTPAELKAMGGQQHPVLGNYGVMSAKNGGINVIITTTVLSFVW
                     YMRGNKVSTVSWAKFGNIFMGCFFFFAYLNIIMLAVYGYYIPANVRVGLSVPQVATTL
                     SCLFFMFALNSVMMKGAKQMGPIEWGKISARSQYALIMLATAFTWMMGLMGYIRSSVR
                     LFWHVNEIMRDNSPWAYTHTVGFAANMISANVLFFWISIMFVFWLGALAAKKAPVEAK
                     AAVPGRAAAPAVGH"
     gene            complement(3463..3585)
                     /locus_tag="PLS35_15060"
     CDS             complement(3463..3585)
                     /locus_tag="PLS35_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20041.1"
                     /translation="MGQTIVYFVVSILICVSFFAAVDHFLMDAQGLDFWYLLRK"
     gene            complement(3911..4864)
                     /locus_tag="PLS35_15065"
     CDS             complement(3911..4864)
                     /locus_tag="PLS35_15065"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI20042.1"
                     /translation="MLETRFKVVFLLAVLFAASLPIIAILRGTISTPLESEVQHSEED
                     SQSSASDSSIPDQPLEEELVRIPAGPFIRGTSQGGFDEQPERKIYLDEFFIDRYEVTN
                     AQYAAFVKATGHRKSGPPSRYAKNTGRMKGVNQPAVYVSWEDAKAYCEWRGRRLPTEA
                     EWEKAMRGTDGRLWPWGNVEMPGGANWGRINDGFEVTAPVGRVRSDASPYGVMDGAGN
                     VMEWVEDWYLEGAYAAAAERNPESPEYGTYKVLRGAGYTSSGSDLRITARSKMMADFR
                     DETIGFRCAQSKAGNAPLSGGVNDGKIIENQSSRGSETRPK"
     gene            complement(4876..5790)
                     /locus_tag="PLS35_15070"
     CDS             complement(4876..5790)
                     /locus_tag="PLS35_15070"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNI20043.1"
                     /translation="MENRGVLIGSIIFVFGSFILMIGGLVYESYKAKQMRQLALSIAT
                     EAKPVAAPLAQDFSMYKTFIGDDGREMVQISEGPFVMGSRDNDSDPDEKPEHQVYLKT
                     YFLDKHEVTQDSYDRFLKMTRRVKRKIEVFEDDPAKLLKPEYPAIAVTWDDAEAYCKW
                     AGKRLPTEAEWEKAARGEGKRRYAWGEEFISGYANIDGAEDGFRYLAPPGSFESGRSP
                     YGIYDMTGNVGEWVADAYDENFYRASPYRDPKGPDQGEQRIIRGGSWRETKRNVRASK
                     RFQAKPWRHDITVGFRCAKDVDADTAVK"
     gene            complement(5806..6195)
                     /locus_tag="PLS35_15075"
     CDS             complement(5806..6195)
                     /locus_tag="PLS35_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20044.1"
                     /translation="MDRPTANTWFDVVLGIVVMATVGALIGSFLGASSIPVTAGLGLA
                     LGAVVGYLGGRRFLVSILVGTVLGGLLAWLIAGVEKVSFGAGAGAAMGGFLGVQISML
                     LDVRAARKAAQLEESEADATHSAVTKL"
     gene            complement(6228..6884)
                     /locus_tag="PLS35_15080"
     CDS             complement(6228..6884)
                     /locus_tag="PLS35_15080"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HNI20045.1"
                     /translation="MRKLVGALGLLSGIALCSILGGAAPASAAETGVMVLEDFQSADQ
                     DGFPVDWQHENQRSQAKGRDAYKLQSEEGKKFLAAKDAGQRIKKRKIDWDPKVYPVLT
                     WRWRLHKAPSNSEPVAALYASLDTDLMFIPVFTKYIWSAGKPVGTFVEGGMFSGSELV
                     VQSGVVPVGEWVEERVNVYEDFKRIHKHEPADKAWGISLFAGPGVEIDFGAVTVGPAK
                     "
     gene            complement(6916..>8609)
                     /locus_tag="PLS35_15085"
     CDS             complement(6916..>8609)
                     /locus_tag="PLS35_15085"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI20046.1"
                     /translation="NHYPQVGSMDSRLLMWFIIQQHTYFGGFVLALPVFCVLLEFLGL
                     VARNPAMALRYDGLAQDLLKVALLALSVTAAVGSLMLTMFITLYPSFMQYMGGTFKGM
                     MPLYALVFVGTTFLTIAYYYSWERMAAPGTKWVHLSIGLLAVVCGTSLLLLANSWSAF
                     MMAPSGVDGQGRYLGNPWHLLRSALWNPLNLHRFLADIMSGGAVVLAYACYRFFSGKS
                     QEERAYYDWVGYVFLFVTVCALLPMPFAGYWLMRSVYAYSQSMGVTMMGGLLTWLFVV
                     QALLIGALFLGVNYYLWQSMGRIRGGERYQPYYQYLLLALTGCLFIWLTPHTLLMTGT
                     EVKAMGGAQHPVIGNYGVMSSKNGAVNVMICITALSYIYYRRANRTMTISWARVGNVI
                     LGALFGLGVAHIIGLSIYGFYLPASLRVGLSGPQAVTTLLVVTLGLLLNRRMLQGATI
                     HGPVQWGQISVRGMVGLFGLAAAFTWVMGLMGYIRSSGRLAWHVNELMPDTSPWAFTP
                     TLGFAAKMVTINMVLFWGAVFFLFWVCQRGQQPVMHEDVSAEKEAGFLPSPSHEG"
BASE COUNT         2156 a         2591 c         2013 g         1849 t
ORIGIN      
        1 gccccgactg acattaatgc tttcatgtcc tcatctccct ttcttccgca atagcgacca
       61 gatacctatt cgcttgattt aggaggcctt tggaggctct tttccttcct gaatctgcga
      121 aaggtaatct acaagcttct tgagtgcgcc agcgctaagc ttttggccaa acaccttcgg
      181 catcgtgtta tcagggaatc ccttgacaac atatgcgctg ggctcaacga ttgattccat
      241 gatgtagtca ggtacactct tagccttacc tttgtaatcc ttatccttga tccggagagg
      301 agcattggta ccttcgacca acttaggacc gatcgtgccg gtcgcccctg caatgccagg
      361 aattgtatgg caggctacgc attgaccctt ggcaaaaatt tgatcaactg gttcagttcc
      421 atctgccatc aaagaagcgc cacctccccc agcagcctta tcaccttctg tcggcggctt
      481 tggtcgatct gactcaggaa tgaacttctc gtacgatttc acaatctcat caaagcccgg
      541 agcctcacgc ccctcacgaa cgtacagcca ggtgtccacc gctgccaatt cagggaggct
      601 caacgaaatt ggcggcttgt ggatagaagg cattgggctt tctttgtcgt tggtgccctt
      661 cacgccgaaa cctgcaacaa cgaagcagct cgggcaggca tgcgattcag cgatgtactg
      721 gataccattt tctgcggtac cagaaccggc aaacgcttct ttctggttgg aatcgcgagc
      781 ggcagggttc cctttgtggt atcgaggatc gttgatctga gtttcagccc gctcaggaat
      841 gccgtccaaa tttggcgcac gctctcccaa catacctttg tggaatgcgt ggcagagcgg
      901 gcactgcccc ttaccgattg cgccctgcac cttattctgc ccgacaccac cgaagatgat
      961 cttctcacct tcgtcggcaa gttcggtttg cggcatgttg ctgaagtcct tcttctcttc
     1021 aatcggaggg aatccaccct ctacctgagg cagccagttc ccataaccgg agagtgccgc
     1081 agcgatgaag aacatgaagc ccccgatttt cagtaacgcg ctgatattcg tgaaatacag
     1141 ggccatcatg aatgtcattg acgtgatcaa caccaacgac accggcaaca gtctcgattc
     1201 accgaagaag ttcattttgt agttagcaat ggccataaac aggagcaacg cgccccaaat
     1261 gccaatgaac gtcttgaaga ctgcccgctt atttgccacc gggtccgaaa tggaggcctt
     1321 aaaatagaac atcaggccga ttaataggcc caatgctggc cagcccatcg ataaggctga
     1381 tttaatgagt tcaatcacag ctcaaacctc ctgcaggagg ggaagcctcc ggttgccatg
     1441 caaccggaga cgatccctca ttgattcatt gttcgtgttt tgattagtga ccaactgctg
     1501 gcgccgcagc acgaccaggg acagcagctt tcgcttctac tggagccttt ttcgccgcta
     1561 gggcacccaa ccaaaacaca aacatgatgc tgatccagaa gaacagtacg tttgcagaaa
     1621 tcatgtttgc cgcaaaacct accgtatgag tgtaggccca tggggagttg tcccgcataa
     1681 tttcattcac gtgccagaaa agcctgaccg aggaccggat gtatcccatc agacccatca
     1741 tccacgtaaa cgcagtagca agcatgataa gggcgtactg agatcgcgcc gaaatcttcc
     1801 cccattcgat cggccccatc tgcttagcac ccttcatcat gacgctgttg agcgcaaaca
     1861 taaagaagag gcaggacaga gtggttgcca cctgaggcac agacagaccg acacgaacgt
     1921 tcgccggaat gtagtagcca taaaccgcca acatgataat gttgaggtag gcaaagaaga
     1981 agaagcagcc catgaagata ttgccaaact tcgcccaaga taccgtcgag accttattgc
     2041 ctcgcatgta ccacacaaag ctcaaaaccg tggtcgtgat gatcacgttg atgccaccgt
     2101 tcttggctga catgacgccg tagttaccca acacagggtg ctgctgacca cccatggctt
     2161 tcaactcagc tggagtcatg acgatggtgt gcggggtaat aaacacgagg taaccgcaag
     2221 tcaacaagaa taccaagtac ttaatgtagc gctgatactt ttcagcgccc cgcatgcgcc
     2281 caagcgcctg ccaaaggtag taattcgtgc tcaaaaacaa gattccgatc atggtcgcct
     2341 gaatgatgaa caaccacgca agcaatccac ccatcaacgt gatacccatc tgttgacgat
     2401 aggcatacac ctcgcgcatc agccagtagc ccgcgaaggg aagaggaatc aggaacgcca
     2461 caccaagggc catggcgata taccccatcc agtcgtagtg agcacgttcc tcatcagtct
     2521 tggccgacaa gaaacggtaa gccgcgtaag cggccacgac gccgcctcca aacgccatgt
     2581 tgccgaggat gcggtgcaga ttgagcggat tccaaagcgc ggtatggatc acgtgccaga
     2641 tatttccgag ataccggccc tgttcatcaa cgccggccgg tgacatcatg aaaccgatcc
     2701 aggaattcgc caagaacatc aggatggtac caatcacgtt cagaacaact gacatgctga
     2761 ggtgaatcca cttaaggaag ccctccttca tcttgtccca accgtaataa tagatgtaga
     2821 gagtgccgct ctccgccacg aacatcaacg catagatgtg catgaccggc ctaaagatac
     2881 cggaaaggta ctggaagaag gccgggtaga gagtcaggaa ggtgaagatc aaaataccgc
     2941 ccaagatggc ggtcaacgaa taagcggtca aactgatctt aataaagtca taggccaact
     3001 ggtcataccg cttggccaac gccttatcct tggtcaccac acccataaac tcgatgacca
     3061 tacagaagat cggcacggcc agaacaaagc taccgtagta gaggtgctgc tggttagcaa
     3121 accagagaag cacccgactc tcgaaattat agcgcggata gtccttctca ctgtccgtcg
     3181 tctttggtgc cggcggccca gagacaatac cttcggtctt gtaatagaca tccttgccct
     3241 tctcaacttt tgcttcacca ccctcttttt tagcgtcggc agccggagcg ccaccatctg
     3301 cgagagcggg gatcgcaacc agaatgggca tgaggaggag gccgatcatc gcgcatagcg
     3361 ccatgaccga catcaacttc gtgcgggtta cagagcccat gggttacctc cttgatacat
     3421 cgcgtatgtg aacaacaaat tcaaagcgtt atctcccagc acttacttac gaaggagata
     3481 ccaaaagtcc aacccttgcg catccatcaa aaagtgatca acggcggcaa aaaacgagac
     3541 gcagatcaag atcgatacga cgaagtacac aattgtttgg cccatcacaa acccccttca
     3601 acttgataaa cattttctaa ggccgggatc aggatcagca aggacactga atccctccga
     3661 accagtaaaa aaaccagagg ctcactgcca gggtgatgta gaaaatcatt ttcccgatct
     3721 tgattgagac gtcgtcctga gcagcttcca tcttgattcc cccaacggtt taagtacgta
     3781 ttttaaattg acacaccaac tatgctgtgt taatcaaaat ccctcaccca actgattttt
     3841 aaagggattt ttatggcaaa aaactcaaaa gtgttcgaca ttatagtttt ggccggtatg
     3901 gttgtcaata tcattttggc cgtgtttctg atcctctact actttgattt tctatgattt
     3961 tcccgtcgtt cacgccccca gacaaagggg cattacccgc ctttgattga gcgcaccgga
     4021 acccaatcgt ttcatctcga aagtccgcca tcattttgct acgagcggtg atacgcaggt
     4081 cagatcctga actcgtgtat cccgctcccc tcagcacctt gtaggttcca tattcaggac
     4141 tttcgggatt tcgttccgcc gccgcagcat acgccccctc aaggtaccaa tcctctaccc
     4201 attccatcac gttcccggcc ccatccatca ctccataagg actggcatca cttcgtactc
     4261 gtcctaccgg ggccgtcacc tcgaaaccat cgttaatgcg gccccaattc gcaccgcccg
     4321 gcatctccac gtttccccag ggccacaggc gaccatcggt accccgcatg gctttttccc
     4381 actctgcttc ggtcggcaac cgccggcctc gccactcgca gtaggctttg gcatcttccc
     4441 aggagacata gaccgcaggc tgattcaccc ctttcatacg cccagtattt ttcgcatatc
     4501 gcgatggagg ccctgacttg cgatggcccg tcgccttcac aaaggccgca tactgcgcat
     4561 tcgtgacctc gtatcgatcg ataaagaact cgtcaagata gatcttccgt tcgggctgtt
     4621 catcgaagcc cccctgactc gtccctcgga tgaacggtcc cgcaggaata cggacgagtt
     4681 cctcttccaa aggttgatcg gggatagagg aatctgaggc gctgctctga gaatcctctt
     4741 ctgagtgctg aacctcggac tccaatggag tggaaatcgt tccacggaga atggcgatga
     4801 taggcaaact ggcggcgaaa agcaccgcaa ggagaaacac cactttgaaa cgtgtttcca
     4861 gcatggtccg actgattatt tcactgccgt gtcggcatcc acgtctttcg cgcaacgaaa
     4921 cccgaccgtg atatcatggc gccacggttt cgcctgaaac cgcttggatg cacggacatt
     4981 ccgtttcgtt tctctccacg aaccgccacg tataattcgc tgctcgccct gatccggtcc
     5041 tttcggatct cgatatgggg aggcgcggta aaaattttcg tcataggcat ccgccaccca
     5101 ttcaccgaca ttgcctgtca tatcgtaaat gccatacggg ctgcgccccg attcgaatga
     5161 tcctggaggg gccaaataac gaaatccatc ttccgctccg tcgatgttgg catagccgga
     5221 aataaactcc tcaccccagg cgtatcggcg cttcccttcc ccgcgagcgg ccttttccca
     5281 ttccgcctca gtgggcaatc gcttaccagc ccatttgcaa taggcctccg catcatccca
     5341 cgtcacggct atggcgggat attctggctt cagcaacttg gcaggatcgt cttcaaacac
     5401 ctcgatcttt cgttttacgc gtcttgtcat cttcagaaac cgatcatagg aatcttgtgt
     5461 cacttcgtgt ttatccagaa agtaggtctt caaatagact tgatgctcgg gtttttcatc
     5521 cggatcactg tcattatcac ggctgcccat cacaaacggg ccctcagaga tttgcaccat
     5581 ttctcgccca tcatccccga taaacgtctt gtacatagaa aagtcctgcg ccaggggcgc
     5641 agcgacgggc ttggcttcgg tcgcgatgga gagggctagc tgacgcattt gcttggcctt
     5701 gtaggattca tagaccaatc ccccaatcat caaaatgaac gatccgaaca cgaaaataat
     5761 cgacccgatg agtacgcctc gattttccat cgccttactc gcacctcata atttggtcac
     5821 tgcggagtga gttgcgtctg cctcactctc ctctaactgc gcggcttttc tggccgctcg
     5881 cacatccagc aacatcgaaa tctgtacgcc aagaaatccg cccatcgccg cgcccgcccc
     5941 ggccccaaac gaaacctttt caactccggc gatcaaccag gcaagcaacc ctcccaagac
     6001 ggtgccgacc aatatactga cgaggaatct gcgtccaccc agatagccaa ccaccgcccc
     6061 gagagcgagt ccaagcccag cggtcacggg aatggaggag gcaccaagaa acgagccgat
     6121 caaggcgccg accgtcgcca tgaccacaat gcccaagact acatcaaacc atgtattggc
     6181 cgtgggtcgg tccatacgct gcgcgtcctc agtcagatgt ccctgttcta cttggccggc
     6241 ccgaccgtca ccgcgccgaa atcaatctcc acgccggggc cggcaaataa tgaaattccc
     6301 caagccttgt cagccggctc atgcttatga atacgtttga agtcttcata cacattgacc
     6361 cgctcttcga cccactcccc tacaggcacc actccactct gcacgaccaa ttccgaaccg
     6421 ctgaacatgc cgccttcgac gaacgtcccc accggcttgc cagcgctcca gatgtatttc
     6481 gtaaagacag gaatgaacat caggtcggta tccaacgagg catacagcgc tgcaaccggc
     6541 tctgaattac tcggcgcctt gtgaaggcgc catcgccaag tcaggactgg atacaccttc
     6601 gggtcccagt cgattttgcg tttttttatt cgctgccccg catctttcgc cgcaagaaac
     6661 tttttcccct cttcggactg taacttgtat gcgtcccgcc ctttggcctg gctgcgctga
     6721 ttttcatgtt gccaatcgac aggaaaccca tcctggtctg cagactgaaa atcttccagc
     6781 accatcacgc ctgtctccgc tgcagaagca ggcgcagctc ctccgagaat cgaacacaat
     6841 gcaataccgc tcagcagccc taaggcaccc actaatttcc tcatacccat tacggcactc
     6901 cctgatgaac cttgtctagc cttcatgcga aggagatggc aaaaaccccg cctctttctc
     6961 cgcgcttaca tcttcatgca tcaccggctg ctgccctcgc tgacacaccc aaaacaaaaa
     7021 gaacaccgcg ccccaaaaga gcaccatgtt gatcgtcacc atcttcgccg caaaacccaa
     7081 cgtgggagta aaggcccaag gagacgtgtc cggcatcaat tcgttgacat gccaggccaa
     7141 tctgccggag gaacgaatgt accccatgag tcccatcacc caggtgaatg ccgccgccag
     7201 gccgaacagg ccaaccatcc ctcgcactga gatctgcccc cactgcaccg gaccgtgtat
     7261 ggttgcgcct tgcagcatgc gccggttgag caacaatccc agcgtgacca ctagcagcgt
     7321 tgtgactgct tgaggaccag acaatcccac tcgaagactc gccggcagat aaaacccata
     7381 gatcgacaat ccgatgatgt gcgctacccc caggccgaac agcgccccca gaatgacatt
     7441 tcccaccctg gcccacgaaa tggtcatcgt tcgattcgcg cgacggtaat agatgtaact
     7501 caacgccgtg atgcagatca tcacgttgac cgcgccgttt ttcgaggaca tcaccccgta
     7561 attcccgatc accgggtgtt gcgcgccacc catagctttg acttccgtgc cggtcatcaa
     7621 caacgtgtgc ggagtcagcc agataaataa gcatcccgtc aacgccagca acagatattg
     7681 ataatagggc tgatagcgct caccgccccg aatgcgtccc atgctctgcc acaggtaata
     7741 attcacgccc agaaagagag cgccgatcaa cagcgcctgc accacgaaga gccaggtcag
     7801 tagcccaccc atcatggtga cgcccatgct ctggctgtag gcgtagacag aacgcatcag
     7861 ccaatagccc gcgaagggca tgggtagtaa cgcgcagacc gtgacaaata gaaacacgta
     7921 gccgacccaa tcgtagtacg cccgctcctc ctgactcttc ccggaaaaaa accggtaaca
     7981 ggcataggcc agcaccaccg caccacccga catgatgtcc gcgaggaacc gatgaagatt
     8041 gagcggattc cagagggcag agcgaagtaa gtgccatggg ttgccaagat atcgtccctg
     8101 cccgtcgaca ccagagggcg ccatcataaa cgcagaccag gaattcgcca gcagcagtaa
     8161 tgacgtcccg cacaccaccg cgagcaggcc gatcgaaaga tgaacccatt ttgttccagg
     8221 cgctgccatt cgttcccagc tgtagtagta ggcgatggtc aggaaggtgg tgccgacaaa
     8281 gaccaaggca tacagcggca tcatgccctt gaaggtgccg cccatgtact gcatgaagct
     8341 gggatacagc gtgatgaaca tcgtcagcat cagactcccg accgcggcgg tgacggagag
     8401 tgccagcagg gcgaccttca gcagatcctg ggccagaccg tcataacgca acgccatcgc
     8461 gggatttctc gcgactaatc ccaaaaactc caagaggacg caaaagaccg gcagggccaa
     8521 cacgaagccg ccgaagtagg tatgctgttg aatgataaac cacatcaaca accggctatc
     8581 cattgagccg acctgcggat agtggttgt
//
LOCUS       DAOQEW010000163         8580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2777_length_8580_cov_7.926100, whole genome shotgun sequence.
ACCESSION   DAOQEW010000163 DAOQEW010000000
VERSION     DAOQEW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2777_length_8580_cov_7.926100"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2421
                     /locus_tag="PLS35_15090"
     CDS             <1..2421
                     /locus_tag="PLS35_15090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI20047.1"
                     /translation="LRIFMVWCLGGWLGAFSVLVAHAQQPAGDASRTVVGIVQNQDLR
                     RVPQAAVQVKDQEGTVIVTAVANEAGEFTVDLPSQGTYSVSAQMDVDRSEYLILKSGA
                     EPVGPVTLTLAKTNEISLEVVSPLAPIQYKASSETYALSRKEIESLPRGNNNDLQDVL
                     LTIPSAVYGSLKQVHIRQDHANLQLRIDGVPIPDTVSSTFSDVISPRAWDRADIILGG
                     MEAQYGNKAAAVLDITTKSGTKPAFGSVQMMGGSNGTLNPSFEYGGTVGEKFRFYILN
                     SHTATNRGIEPPTLGHSVFHGQSERNQTFIRGDYQHDNKNNFTWLFLNSVAKYQIPTT
                     PGLELDPSGQMLPLLQAGRPGFTPVASQAINEFQKENNQYGHMVWRHDVNANNFFSLA
                     GYMRHTRATFNTDPLNVLAYTADPTQPFSSADQDRNAYSTGLRFDYTYVHSKEHLIKA
                     GFQLDRTQSVNKTRLFTFADDGAGNPTGDLLSLNADNRQIGYRQEFWIQDQWSPTDKW
                     TFNLGLRGDAVQYLRNEGQVSPRLGATYKYDQSNVFHAFYGRMFTPPNLEAISFAKLN
                     TLGTRAQPEDVTNNTVRAERAHYFEVGSYHALNRYATLQFTGWYKLSNFLSDAGQFGT
                     TPLLNYFAFERGWQRGIDGALKLQLTDNLTARGNVAWGQCKGYGLQSGHFLLEAKEIA
                     DINSRGGVFCDHMQTLTSSAVVTYKFLERTTMTGQMLFASGLRTAENEEAKTNSTHSP
                     SYTIYNLSIAHVFPLPWDGQKFLLGFDIINLLDQKYYINQGEGSIGLGVAHAGMPRSF
                     FFRGQWFF"
     gene            2737..3198
                     /locus_tag="PLS35_15095"
     CDS             2737..3198
                     /locus_tag="PLS35_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNI20048.1"
                     /translation="MTNIIDGIRSLTVPMVVLGMWCLLPVYAIAVTGDEATHEADHAL
                     DAEIIELPEVHVHGLPLNKDQQVGPVPKSTPWPAIPSSLEGKPLDDWMKARLLVSKEA
                     QVTVVVLESAKHRELTIAGIIALNKWTFAPQLKGDDPVDGELTVRIHFRSQ"
     gene            complement(3249..4736)
                     /locus_tag="PLS35_15100"
     CDS             complement(3249..4736)
                     /locus_tag="PLS35_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20049.1"
                     /translation="MMIRNVFGLLLPLALTAQLAGYAALVVHAANEKQGAQHYELSRG
                     AGEKRSECVGMVRIGPGIAAMDLEATRTDSDDAQISLRGTRDSFVCEAILHPAMRAVL
                     GRSIVQFHNGTTLPLAQVLRQILEAGKQPRMAQTVTSTALDRIQVHALHAVVVYEGKA
                     HLPDSHPIETPQGTIHITVEKTREPITLFLMSYNPVDWRISAVPGARIGSVILRGYYR
                     QTIRGLDKTVPVNTASTEDDNWDDLFPRTLVTSRDGLIDFKAYVRAMAGVEPATVQSL
                     HTGDSIVIDGKTTLEFPAPIEVPITQPVVFKSESESSARFRGRNMISPDRLTVGYGMS
                     GASTESIATMPHSSGRWYAEFIVHAGADNAVPISWTNVGIRSTANESGGLTSHGGQLA
                     YGVKQYLRNVGGLRDKDVIGLAMDLTRGHLYVRHNGVWVGGSPDSGNPILQIVRDRQY
                     VVAVEVGSPGGDYSKSDTWSGNFGATPFRHPVPDGFLPYEGRRPK"
     gene            complement(4845..6479)
                     /locus_tag="PLS35_15105"
     CDS             complement(4845..6479)
                     /locus_tag="PLS35_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNI20050.1"
                     /translation="MTCFRSRPAPCVSGERADARRFTDLARTAALLLALSLSACSTFP
                     AVDLSPPYEPAQFVVPASWHGSSPFVEAKPSDGELRQDWWTLFKDPVLNGLEAQALTA
                     NPDLQAAAERFVQSRDVMMKARSQYLPRVGLGFDATDNRQSLHRLFRAPDSPLEESSV
                     VGGGLASWEPDFWSALRNATKVEIYRAEERAADFGLARLSLQAEVAANYFALRGLDAQ
                     DAIYTQSIALYTQSLELVNAQFIGQIASALDVARVESLLFSTETRRAQIQGQRQVLEQ
                     AIAILLNVAPASFTIAPVNDLKMATFTIPQTLPSTLLERRPDIAAMERRMAQANRTIG
                     IARAAFYPNVSFRLGGGFEDEAFNLFSLANSFWSYGSGVSLPLFQGGYRRAQLQQSWS
                     AYREIEDRYRSTVLNAFREVENNLSLTNRLTLAANRQDAAVGATLKTQDLTMELYQGG
                     LASSLELIYAQVAMLTARIESVQIKAELLRTSVALIRSLGGGWNRQQLPTDDEIQPFG
                     TFQYVNLDKPPSVRGIDVNAENNRLHNDLTKKPAVR"
     gene            complement(6476..7678)
                     /locus_tag="PLS35_15110"
     CDS             complement(6476..7678)
                     /locus_tag="PLS35_15110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20051.1"
                     /translation="MLKRLSPRPGVLLGIVLLLFYLSYRIYESNVDATTLRKHTLEDT
                     IPTVAITYPKPVPPTETITLPGNIQAWFEAPIYAQVSGYVKMWYKDYGALVQKGDILA
                     EINAPALDAQYAQAKADLAAEQAKYALADLTAKRWVALRKNHAVSEQSISVQEANAKA
                     EAARVKAAEQNVKNFEALIQFKTIVAPYDGVVTVRNINVGDYINKEGNISNRSSITNL
                     FTVADVSMLRLFVNVPAAFGAFLKPGLTADVTVPQLPDRHFTAKFLTVARGFDVSTRT
                     AVTVFTIDNEDRALWPGSYAKVHLTAPVDRNVLTIPSTALVFQEHGTQVAVVAEDDRV
                     QLKPITVSKLMDNAVEVSGGISSQDRIVNNPSAALLEGDKVRVVTPAPGYDLVGTATP
                     LAKEPALK"
     gene            complement(7678..>8580)
                     /locus_tag="PLS35_15115"
     CDS             complement(7678..>8580)
                     /locus_tag="PLS35_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI20052.1"
                     /translation="LMTTAGSQQIDQVYWLDPKTGMSYLINVYTPQAQVNSVNSLRTI
                     PVEPSNDVSDNNHDVQLLGNLADLSAEGTPGVITHANIMPLFDIYVGTEGRDLGGVLA
                     DVETVAKSMEHELPRSAAIEIHGQASLMHDAYFELIFGLLAAIVLVYLLIVVNFQSWL
                     DPFIIITALPGALAGIAWALFLTHTRLSEPALTGAIMTMGTATANSILVVSYARERLE
                     THGEALRAAIEAGTTRFRPVLMTASAMIFGMVPMATGYSQNAPLGRAVIGGLLVATIF
                     TLFFVPCVYAILYDGRSVPAKEQL"
BASE COUNT         1782 a         2419 c         2617 g         1762 t
ORIGIN      
        1 ttgagaatat tcatggtgtg gtgtctcgga ggttggctcg gggctttcag cgtccttgtc
       61 gctcacgccc agcagccggc gggagacgca agccggacgg tcgtcgggat cgtgcagaac
      121 caggacctcc ggcgtgtacc ccaggccgct gtgcaggtga aggatcagga gggcaccgtt
      181 atcgtgacgg cggtagccaa tgaggcggga gagtttacgg tggacctgcc ctcccagggc
      241 acctattccg tcagcgccca gatggatgtc gatcggagcg aatatctcat tctgaaaagc
      301 ggagctgagc cagtcgggcc ggtgacgttg acgctagcca aaaccaatga aatttcactg
      361 gaggtggtgt cgccgctggc gccgattcag tacaaggcct cgagcgaaac ctatgcgctc
      421 agccggaaag aaattgaatc tctcccgcgt ggcaataaca acgatctcca ggatgtgctg
      481 ttgaccattc ccagcgccgt ctatgggtcg ctgaaacaag tacatatccg gcaggaccat
      541 gcgaatctgc agctgcgtat cgacggggtt ccgataccgg ataccgtctc atcgacgttt
      601 tcagatgtca tcagtcctcg cgcctgggat cgagccgaca tcattctggg cgggatggaa
      661 gcgcagtacg gcaacaaggc ggcggcggta ttggatatca cgacaaagag cggaaccaag
      721 ccagccttcg ggtcggtgca gatgatgggc ggctcgaatg gcacgctcaa tccgtcattc
      781 gaatatggcg gaacagtcgg cgaaaaattc cggttctata ttctcaacag ccatacggcg
      841 acgaaccggg gcatcgagcc gccgacgttg ggccattcgg tgtttcatgg ccagagcgaa
      901 cggaatcaga cgttcatccg cggcgattac cagcatgaca acaagaacaa cttcacctgg
      961 ttgtttttga attccgtcgc gaagtaccag attccgacca cgccgggctt ggagctggat
     1021 cccagcgggc agatgttgcc attgctgcaa gcagggcggc cggggtttac gcccgtggcc
     1081 tcgcaggcca tcaatgagtt ccagaaggag aacaaccagt acggccacat ggtgtggcgc
     1141 cacgacgtga acgccaacaa cttcttcagc ctcgccggct acatgcggca cacgcgggcc
     1201 acgtttaata ccgatccgtt gaatgtcctg gcgtatacgg cggacccgac tcaacccttc
     1261 tcctctgccg atcaggaccg taacgcctac tcgaccgggc tgcgattcga ttacacctac
     1321 gtacacagta aggagcatct catcaaggcc ggtttccaac tcgatcgcac gcagtcggtg
     1381 aacaagacca ggctcttcac ctttgccgat gacggggcgg ggaatccgac tggcgacctg
     1441 ctgagtctga acgccgataa ccgtcagatc gggtatcgac aggaattttg gattcaagat
     1501 cagtggagtc ccaccgataa gtggaccttc aacctcggcc tgcgtggaga tgcggtgcag
     1561 tatctccgga atgagggcca ggtcagcccg cggctcgggg ccacctataa gtatgaccag
     1621 tcgaacgtgt tccacgcgtt ttacggacgg atgttcacgc cgccgaatct ggaagcgatc
     1681 tcatttgcga agctcaatac gcttgggacc agagcgcaac cggaggatgt gaccaacaac
     1741 acggtgcgcg cagaacgggc gcattatttt gaagtcggca gttaccacgc gttgaatcgc
     1801 tatgccacgc tgcaattcac cggctggtac aagttgagca acttcctgtc ggatgccggg
     1861 cagttcggga cgacgccgct cttgaattat ttcgcgttcg agcgcggctg gcagcgcggg
     1921 atcgacggcg cgttgaagct gcaactcacc gacaacctga cggcgcgcgg caacgtggcc
     1981 tggggccaat gcaaaggcta tggcttgcag tcggggcatt tcctgttgga ggcgaaggaa
     2041 attgccgata tcaattcccg tggcggggtg ttctgcgatc acatgcagac gctgaccagt
     2101 tccgccgtcg tgacgtacaa attcctggaa cggacgacga tgaccgggca gatgttgttc
     2161 gcgtcgggat tgcggacggc cgagaacgag gaggcaaaga ccaattcgac gcacagtccc
     2221 tcgtatacca tctacaactt gtccattgcc catgtgtttc cattgccgtg ggacggacag
     2281 aaatttttgc tgggcttcga catcatcaat ctgttggatc agaagtatta catcaaccag
     2341 ggagagggca gtatcggtct gggtgtggct catgcgggga tgccgagatc gttcttcttc
     2401 cgtggccaat ggttcttcta ggtcagatgc cgaacaaggt cgccagcagc agcgttctca
     2461 gtcactcgtc tccctgcgac gtaccgcaag ggtacgcctc agtcgccgag ttccctgcgg
     2521 ccttgctgga caaccttgtt gggcatctgc ttcggactgt gggaaaatgc ggccttgctg
     2581 gatgacccgt ttgagcaacc gtttccgaca ggaaggttga aggtgtgcgg cctagctgac
     2641 aaccttgttg ggcatctgtt ccagactgtg agaaaatgtg gccttgctgg atggcccgtg
     2701 tgaacatcat catcgtgaaa ctggttggaa gacgatatga cgaacatcat cgatggaatt
     2761 cgatcactga cagtacccat ggtagtgctc ggcatgtggt gcctgctgcc tgtatacgcc
     2821 atcgcagtaa caggtgatga ggcgacgcat gaggcggatc atgcgctgga tgcggagatc
     2881 atcgagctgc ccgaagtgca tgtgcacgga ttgccgctga acaaggatca gcaggtggga
     2941 ccggtgccga aatcgacacc atggccggcc attccctcgt cgctggaagg gaaacctctc
     3001 gacgattgga tgaaggcgcg cttgctcgtg tcaaaagaag cgcaggtgac ggtggtcgtg
     3061 ttggaatcgg ccaaacatcg cgagctcacg attgccggca tcatcgcgct caacaaatgg
     3121 accttcgccc ctcagctcaa gggcgacgat ccagtcgacg gggaactcac cgtgcgcatt
     3181 cactttcgat cgcaatagat acgtctcgac aagcgagccg caagggtccc ttgcgggacg
     3241 agcacagtct acttcgggcg gcgaccttcg taaggcaaaa acccatctgg aacaggatgc
     3301 cggaagggtg tcgccccaaa attgccgctc cacgtgtccg acttactgta atccccaccg
     3361 ggcgagccga cctctactgc gacaacatac tgtcggtccc tgacaatctg caggattgga
     3421 ttgccgctgt cgggcgagcc tccaacccat acgccattgt gtcgaacata gagatggcct
     3481 cgcgtcaagt ccatggcgag tcctatcaca tctttgtcgc gcagcccgcc cacattccga
     3541 agatactgct tcactccgta tgccaattgc ccgccatgtg aagtcagtcc gcctgactcg
     3601 ttggccgtcg atcggatacc gacgttggtc cacgagatcg ggaccgcgtt gtcggcgccg
     3661 gcatgtacga tgaattcggc gtaccatctt ccgctgctgt gcggcatcgt ggcgatgctt
     3721 tcagtcgaag cgccgctcat gccatatccg acagtcagac gatccggaga aatcatgttc
     3781 cgcccgcgga atcgtgccga ggattcgctt tccgacttga ataccactgg ttgcgtgatc
     3841 ggcacctcga ttggagccgg aaactccagc gtcgtctttc cgtcgatcac gatcgaatcg
     3901 cctgtgtgga ggctctggac cgtcgcgggt tcgactccgg ccatcgcgcg cacataggct
     3961 ttaaaatcga tcaggccatc tcgggatgtc acgagggtcc gaggaaacag gtcgtcccag
     4021 ttgtcatcct cagtggatgc ggtattgacg ggaacggttt tgtccagtcc tcgaatcgtt
     4081 tgacggtaat agccgcggag aatcaccgac cctatgcgcg cgccgggtac ggcggagatc
     4141 ctccaatcga cagggttata tgacatgaga aagagcgtga tcggctcgcg cgtcttctct
     4201 accgtgatgt gaatggttcc ctggggagtc tcgataggat gagagtcagg gagatgggcc
     4261 ttcccctcgt aaaccaccac ggcatgaagt gcgtgaacct gtatcctatc gagcgcggtg
     4321 gaggtcacag tttgcgccat gcggggctgc ttacccgctt caagaatctg gcgcaatacc
     4381 tgcgcgagag gcagagtggt gccattgtgg aactgaacaa tgctgcgtcc gagaacggcc
     4441 cgcatagccg ggtgaagaat ggcttcgcac acgaaagagt ctctggtccc tcgtagagaa
     4501 atctgcgcat catccgaatc cgtgcgggtt gcctccaaat ccatggctgc tataccggga
     4561 ccgattcgta ccattcccac gcactcggat cgtttctctc ctgcccctct ggacaactca
     4621 tagtgctgag cgccctgttt ctcattcgca gcatgaacga caagcgcagc gtagccggcc
     4681 agttgcgctg tcagtgcaag cgggaggaga agtccgaaaa cgttccgaat catcatgatt
     4741 gtgcctcaat ggtggcagga agggctgtac tctttccaaa cagagatacc acgagagccg
     4801 ctctccacca atcaggaccg gcttttgtgt gtgatggaca gctactaccg aacggcgggc
     4861 ttcttggtga ggtcgttgtg aaggcgatta ttctccgcgt tgacgtcgat gccgcgaact
     4921 gatggtggct tgtcgagatt cacgtattgg aaggtcccga agggttgaat ctcatcatcg
     4981 gtcggcaatt gctggcggtt ccagcctcct ccgagcgagc ggatgagggc caccgaagtg
     5041 cgaagcaatt cagccttgat ctgcaccgac tcgatccgcg cggtcagcat ggcgacctgg
     5101 gcgtaaatga gctcgaggct ggaagccaga ccgccctgat agagctccat agtgagatct
     5161 tgcgtcttga gggtcgctcc aacggcggca tcctgcctat tggccgcaag ggtcagccgg
     5221 ttggtcagac tcagattatt ctccacctcc cggaaggcgt tgagtacggt cgatcggtac
     5281 cgatcttcaa tttcacgata ggcagaccag gactgctgca attgcgcccg gcgataccca
     5341 ccctggaaca gcggcagcga gacgccggag ccgtaggacc agaaactgtt cgcgagcgag
     5401 aacagattga aggcctcgtc ctcaaacccg cccccgagtc gaaacgacac attgggataa
     5461 aacgcggcgc gcgcaatccc gatggtgcga ttggcctgcg ccatgcgccg ttccatggcg
     5521 gcgatatccg gccggcgttc caataaggtt gacggaagtg tttgggggat cgtgaaggtg
     5581 gccattttga ggtcgttgac cggcgcgatc gtgaaggagg ccggtgccac gttgagcaag
     5641 atggcgatgg cttgttccaa cacctggcgt tgcccttgga tttgggcacg cctcgtctcg
     5701 gtgctgaaca acagggattc gacgcgagcc acgtcgagcg ccgatgcaat ctggccgatg
     5761 aactgggcgt tgacgagttc cagcgactgg gtgtacagcg cgatcgattg ggtgtagatg
     5821 gcatcctgcg cgtcgaggcc ccgcaacgca aaatagttgg ccgccacctc cgcctgcagg
     5881 ctcagccgtg cgagtccgaa gtcggcggcg cgctcttccg cccggtagat ctctaccttt
     5941 gtcgcgttgc gcagcgccga ccagaaatcc ggttcccagg atgcgagccc gccgcccaca
     6001 acgctcgatt cttccaatgg actatcgggc gcgcgaaaca gccggtggag ggactgccga
     6061 ttatcggtgg cgtcaaagcc caagccgaca cgaggcagat attgcgatcg tgccttcatc
     6121 atcacatcgc gggattgcac gaaccgttca gcggccgcct gcaggtcagg attggccgtc
     6181 aacgcttggg cttccaaccc gttcaggacg ggatctttga agagagtcca ccagtcctga
     6241 cgcaactcgc catcggacgg cttcgcttcg acgaatgggc tcgacccatg ccatgaggcc
     6301 gggaccacga attgagccgg ctcgtatgga ggggagagat cgacagcggg aaaggtactg
     6361 caggccgaca ggctgagcgc cagcaggagc gcggcggttc gggcgaggtc tgtgaatcgg
     6421 cgtgcgtccg ctcgttcacc tgatacgcat ggggctggcc tggatcggaa acaggtcatt
     6481 tcaacgccgg ttccttcgcg agtggcgtcg cggtgcccac aagatcatac ccgggcgccg
     6541 gtgtgacgac gcggaccttg tcgccttcca gcaaggcggc gctcgggttg ttcacgatgc
     6601 ggtcctgact ggaaatccct cctgacactt cgacggcatt gtccatgagc ttgctcacgg
     6661 tgatgggttt cagctgcaca cgatcgtcct ccgccaccac ggccacttgc gtcccgtgct
     6721 cctggaacac caacgcggtg gaaggaatag tgagcacgtt ccggtcgacc ggcgcggtga
     6781 gatggacctt ggcataggac cccggccaca acgcccggtc ttcgttgtcg atcgtgaaga
     6841 ccgtgacggc cgtgcgtgtg ctgacatcga acccgcgggc gaccgtcagg aatttcgcgg
     6901 tgaaatgacg atcgggtaat tgcggtaccg tcacgtcagc cgtcagtccc ggcttgagaa
     6961 acgctccgaa ggctgccggt acgttgacga atagccgcag catgctgaca tcggcgacgg
     7021 taaacaaatt ggtgatggag ctccggttgc tgatgttgcc ttctttattg atgtagtcgc
     7081 cgacattgat gttgcggacg gtcaccactc cgtcataggg cgcgacgatg gttttgaact
     7141 ggatcagcgc ttcgaaattc ttcacgttct gctccgctgc cttgaccctg gcggcttcgg
     7201 ccttggcatt ggcttcctgc acggagatgg attgttcgga gaccgcatgg tttttgcgca
     7261 gcgcgaccca gcgtttcgcc gtcaagtcgg cgagggcata tttggcttgt tctgcggcga
     7321 gatccgcctt cgcctgggca tattgcgcat ccagggcagg cgcgttgatt tcagcgagga
     7381 tgtcgccctt ttgcaccagg gcgccgtagt ccttgtacca catcttgacg tagccggaga
     7441 cctgcgcgta gatcggcgct tcgaaccagg cctggatatt accgggcagg gtgatcgttt
     7501 cggtcggcgg caccggcttg gggtaggtga tggccactgt ggggatcgtg tcctccagcg
     7561 tgtgcttgcg caaggttgtg gcgtcgacat tgctctcgta gatgcgataa ctgagataga
     7621 acagaagcag gacaataccg agtaggactc cagggcgtgg agagagacgc ttcagcatca
     7681 gagttgctcc tttgcgggaa cgcttcgccc gtcatacaga atggcataga cgcacggcac
     7741 gaaaaacaag gtgaagatcg tggccacgag caggccgccg atcacggcac ggccgagcgg
     7801 ggcattctga gaatatcctg tcgccatggg gaccatgccg aagatcatgg ccgaagcggt
     7861 catgagtacc gggcggaagc gggtcgtgcc ggcttcaatg gccgcccgca gcgcttcgcc
     7921 atgcgtttcg agccgttcgc gcgcgtagga gacgaccaga atggaattgg ccgtcgctgt
     7981 tcccatggtc atgatggctc ccgtcagcgc cggttccgag aggcgcgtat gcgtcaggaa
     8041 caaggcccac gcgataccgg ccaacgcacc aggcaaggcg gtgatgatga tgaacggatc
     8101 gagccaggac tgaaagttga cgacaatcag caaatagacc agcacgatcg cggccagtag
     8161 tccgaagatc aactcgaaat acgcgtcgtg catcagcgaa gcctggccat ggatctcgat
     8221 ggcggcgctg cgcggcaact cgtgctccat gctcttggcg acggtttcga catccgccaa
     8281 tacgccgccg aggtcgcgcc cttccgtgcc aacgtagata tcgaacagcg gcatgatgtt
     8341 cgcgtgggtg atcacgcccg gtgtgccctc ggccgagagg tcggcgagat tgccgagcaa
     8401 ttgcacatca tgattgttgt ccgacacatc attcgaaggt tcaacgggaa tggttctgag
     8461 gctgttaacg ctattgacct gcgcttgcgg cgtatacacg ttgatcagat aagacatgcc
     8521 ggtcttggga tcgagccaat acacctggtc gatctgttgg ctgccggcgg tcgtcatcag
//
LOCUS       DAOQEW010000164         8560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2786_length_8560_cov_7.237155, whole genome shotgun sequence.
ACCESSION   DAOQEW010000164 DAOQEW010000000
VERSION     DAOQEW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_8560_cov_7.237155"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..>1589)
                     /gene="malQ"
                     /locus_tag="PLS35_15120"
     CDS             complement(183..>1589)
                     /gene="malQ"
                     /locus_tag="PLS35_15120"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNI20053.1"
                     /translation="LLINPLHAVPPLVPQEASPYSPSSRRFLNPLYLRIEDLPEAKKL
                     GQELSQLAAMGRALNTDRHIDRDRVYHLKQEALQLLWNSFEGDEDFDRFQSAGGRSLR
                     QYALHCCLVERYGSDWRRWPAVYRHPDSAEAEPALPAEVDRITFHMWQQWLLGRQLTD
                     AGQIIPLMQDLPIGFSPDGADAWTWQDVLAAEMTVGAPPDLFNATGQDWGLPPFIPHK
                     LRATGYQPFIETIRSIICRAGGLRIDHVMGLFRLWWVPRGSPPKDGAFVRYAAEELLA
                     ILAVESTRAQAIVVGEDLGTVEEGVRETLAEQNVLSYRLLWFEDQPPSQYPWKALASV
                     TTHDLPTLAGVWTGADLESQRKSGASPDEKAQEQFRTKLVEVSQVEDQTNLEEVITKT
                     FSKLAESPCAVVMADLEAACAVTERPNMPTSGGNYPNWSLALPCSLEELTAAERPAKL
                     AHILNRRVGDTIGTSSRP"
     assembly_gap    1590..1689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2216..4075
                     /gene="treZ"
                     /locus_tag="PLS35_15125"
     CDS             2216..4075
                     /gene="treZ"
                     /locus_tag="PLS35_15125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HNI20054.1"
                     /translation="MSSVRQRCGAHLSADGRMEWRVWAPYAKTVDLIVIDGAARRVEA
                     MTAEPHGYFFIRGGSASHGQQYAYSLDKGPDRADPASRWQPDGVTQPSAIVLPSRFQW
                     TDDRWEGIARDQLVFYEVHVGTFTAEGTFDAIIARLPSLRQLGVTALELMPVGEFPGT
                     RNWGYDGVYPFAPHHSYGGPEGLQRLVNACHEQGLACVLDVVYNHFGPEGSVQYEFGP
                     YFTDHYKTPWGRAVNFDGPGSEGVRAYVIENVRMWISDYHIDGLRLDAVHAIYDFGAT
                     HLLKEIKQAATEAAGDRKFPAYVVAESDLNDVRILLREDQGGYELDAQWSDDFHHTVH
                     ALLTGERQGYYSDYGTAEQLSAVLEKTFAIDGGFSRHRNRCHGAPAAGLPGDRFIGSI
                     QNHDQVGNRAGGERLSHLLPPPLQRLAAAFLLLAPHLPLLFMGEEYGEARPFQFFCSF
                     DGEELIESVRKGRRQEFEAFHTSDAQVTDPQDPSTFDQSRLTWRWDEVPHQAGLRRLY
                     TDLLEARRVWPAMRNYAERMSRLHRGADGAVVLELVRGGTKAAQAHTIQAFFNPTESV
                     QNLPARALPTLLFSSEWERYDGVRASSSESGQLLPFECAIFGPSDWKRYAGLE"
     gene            4532..5368
                     /locus_tag="PLS35_15130"
     CDS             4532..5368
                     /locus_tag="PLS35_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI20055.1"
                     /translation="MTCLAQTGAPVQEQPPDVGPRTRDQASQERVVTRDRAGDRVVVD
                     ERVVERRREGEVYVAGYGGFTWGHSFSDLEGRGTIAGQNFGGRDLANSGVYGAKIGYF
                     HPGRLNWLGIEVEGFNSTPHIEQGGGLPGSHLRVTTLAFNVIARTKVGCRNRDDVRHR
                     DEVRHRDDGTVVESDYDRRQWSPLDENLRCPLQLYAGAGPGIFFAETSNQFGRSTDNG
                     RIGLNALAGAKYFMTRNLAVFAEYKFNYAQFDFSQMQGTGAGFNGEYKASHVVGGLAL
                     HF"
     gene            complement(5512..5841)
                     /locus_tag="PLS35_15135"
     CDS             complement(5512..5841)
                     /locus_tag="PLS35_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNI20056.1"
                     /translation="MMPPQPEAGQPKIIVHIDPDLEAIVPGFLANRRRDLVTIEACLK
                     QSDLNTIRILGHRMKGDGGGYGFDQISSIGDDLEQAVMVQNGPLIEKQVALLKDFLSR
                     VEVVYTR"
     gene            complement(5838..>6107)
                     /locus_tag="PLS35_15140"
     CDS             complement(5838..>6107)
                     /locus_tag="PLS35_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20057.1"
                     /translation="AKFQNGHYDLILMDMQMPVMDGLTATRTIRRWEQEQGLPAVQIV
                     ALTALALKEESARIFEAGCNAHMTKPLKRTTLLELLSAYEKTHAQ"
     assembly_gap    6108..6207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6208..7470)
                     /locus_tag="PLS35_15145"
     CDS             complement(<6208..7470)
                     /locus_tag="PLS35_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20058.1"
                     /translation="MAKRISQTRRTTSTTPKRTLRTSSRPQTALQREQAARRRAERAL
                     DRASKELQRRVEELQRAKDAADVANRAKSDFLASMSHEIRTPMNAIIGMADLLWDTTL
                     TPEQRKYLRIFRRAGASLLSLINDILDLSKVESGRLDLDSIDFDLSELIDKASEMLAL
                     RANEKGLELVCHLSNDVPCHLIGDPNRLYQILLNLLGNAIKFTEKGSVVMTITNDPDC
                     QTPGAIRFSVTDTGIGVPHDKMTAIFDTFTQAHSTIARRYGGTGLGLSISQQLAALMG
                     GKIWVESTLGKGSTFHCLVRLGVQAAPAPSKGAGALNLSGVRCLVVDDYPTNRLILRE
                     TLFAWGATVVEAESGEQAMEVLESALTSQTSFDLLLLDCRMPGMDGFEVIERIHADPR
                     LRNLTIIMLASDRWADDIAKTYDLGLGGY"
     gene            complement(7560..8558)
                     /locus_tag="PLS35_15150"
     CDS             complement(7560..8558)
                     /locus_tag="PLS35_15150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNI20059.1"
                     /translation="MLIVDDSTDDRLLIQSILANAGYEETFVAESAAAAFRLLGLDGM
                     RQMAGRVDLILMDIVMPSTNGIEACRQIKAVERYRDIPIIMVTVKTDPVDLQLAFAAG
                     AIDYVAKPISKIELLTRVRCVLRLVHEIERRRAREQELLEVMRQLQEANQMLLRLSCL
                     DGLTGITNRRQFDDFLDQEWRRAARESTPLSLIMLDIDFFKTYNDSHGHQAGDECLRQ
                     VAQLISSHVNRPGDLVARYGGDEFVIVLPGTTVDGAAQVADTLRRKVCEAEMKHPNGD
                     PVTISLGFASTVPSRVAAPTDLIRAADQALYQAKQEGRNRAKQAGVLSLVHHTHKD"
BASE COUNT         1646 a         2294 c         2610 g         1810 t
ORIGIN      
        1 tcaggtcgaa gagccgatct acattcggcc ggccgagcgc gtgaactggc tgtaaattct
       61 gcctagctga gagcgggtcg ccgctctcag ctaggaaggg tgattgctct attgatggag
      121 gccatacacg caacgatgtc tcgcaggcgg cgtgggatcg tggtcgcgat ggcgtgaaga
      181 ggttaggggc gagaagaagt gccgattgtg tcaccgacac gccggttcag tatgtgtgct
      241 agcttcgcgg gccgttcagc cgctgtgagt tcctccaggg aacagggcag ggcaagcgac
      301 cagttcggat agttgccgcc cgaagtgggc atgtttgggc gttcggtcac tgcgcaggcg
      361 gcttcaagat ccgccatcac caccgcgcag ggtgactccg caagtttcga gaacgtcttg
      421 gtgatcacct cctcgagatt cgtctgatcc tctacctggc tcacctccac aagctttgtc
      481 cggaactgct cttgggcctt ttcgtccggt gaggcgccgg atttgcgctg tgactcgagg
      541 tccgcccctg tccagacgcc tgctaaggtg ggaaggtcat gggtggtgac ggaggccaac
      601 gctttccagg ggtattgcga cgggggctga tcttcgaacc aaagcaggcg ataggacaag
      661 acattctgtt cggcgagtgt ctctcgcaca ccttcctcca ccgtcccgag gtcttctccg
      721 acgacgatgg cctgcgcgcg agtgctttcc acggcgagga tcgccaacag ctcctctgcc
      781 gcataccgca cgaaggcacc gtccttgggc ggactgcctc gagggaccca ccacaggcga
      841 aagagcccca tcacgtgatc gatgcgtaag ccgccggcac ggcagatgat ggagcggatc
      901 gtttcgatga acggttggta gccagtcgcc cggagtttat gcggaatgaa tgggggaagt
      961 ccccaatcct ggcccgtcgc attgaagaga tcaggcggcg ctccgaccgt catctcggct
     1021 gcaagcacgt cttgccatgt ccaggcgtcg gcgccgtccg gtgaaaatcc gatgggtaga
     1081 tcctgcatca aggggatgat ctgccccgcg tcggtgagtt gtcggccgag cagccactgt
     1141 tgccacatat gaaatgtgat gcggtcgact tctgcgggca gggccggttc ggcctcggct
     1201 gagtccgggt gacgatagac ggcaggccag cgacgccagt cggagccgta acgttcaacc
     1261 agacaacagt gaagtgcata ctggcgcagc gagcgccccc ctgcggactg aaagcggtcg
     1321 aaatcttcgt ccccttcaaa gctattccac aacagttgta aggcctcttg cttgagatga
     1381 tagacgcgat cgcgatcgat atgacgatct gtgttcaagg cccgacccat ggctgccaat
     1441 tgtgacaact cttgcccaag tttcttggct tcgggcaggt cctcgatgcg caggtagagc
     1501 gggttgagaa agcgacggct ggagggcgag taggggcttg cctcctgcgg aacaagcggc
     1561 ggaacggcat gtaaaggatt gatcaggagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnt tggacacctt ccgcatcttg cccgcggcat cttcgtattg ggcttgaatg
     1741 ccccatgcat cggtcttcat gcctgatggt ctccttggct cttcctctcc tgtccggttc
     1801 gtgccgtcac tcaatcacgg tctccttgcg ctgtcttcct gccaggcctt gggccatggc
     1861 ctaaagcctc cgtgaggtaa aggtgcaacg ccacgtctgc ggacaatgat gtcgttgatt
     1921 gtaggacggc gctgcgttat gcaatgtcca ggctcggctt gtgggcgagt gattccacgg
     1981 gattttatga gagaaaagat cagatcgcga cggatcgcgg tggccttttg tccctagaca
     2041 gacaacttgt ccccgttgtt cggacactca ttctcctaaa agtgagacca ggcaagagcc
     2101 gtcagcggag gagagagacg ccgtgtgtgg cgcatgcctt gcacagggct acccaacctg
     2161 tagcgttctt cattcatgta ggtagtttcc gaacatagga tgtggaggag atgggatgag
     2221 cagtgtaagg cagagatgcg gcgcgcatct gtcggccgat gggagaatgg aatggcgtgt
     2281 ctgggctcct tacgcaaaga cggtcgatct catcgtgatt gacggcgcgg ctcggcgtgt
     2341 agaagcgatg accgccgaac cgcatggata ctttttcatc cgtggaggca gtgcgtccca
     2401 cgggcagcaa tatgcgtatt ccctcgataa ggggcccgac cgtgcggacc ctgcatcacg
     2461 ctggcaaccg gatggggtaa cgcagccgtc ggctatcgtt ttgccttcgc gctttcagtg
     2521 gacagatgac cggtgggagg gaattgcgcg ggaccagctc gtcttctatg aagtgcatgt
     2581 cggaaccttc acggctgaag gaacgtttga tgccatcatt gcccgccttc cctcattgcg
     2641 gcaactcggt gtcactgctc tggagctgat gcctgtgggg gaattcccgg gcaccagaaa
     2701 ctggggatac gacggggtct atccgttcgc gcctcaccac agttacggcg ggcctgaagg
     2761 cctccaacgg ctcgtcaacg cctgccacga acagggcctg gcttgcgtgc tggacgtggt
     2821 ctataaccat tttggccccg agggcagcgt tcaatatgag ttcggcccct atttcactga
     2881 tcactacaag acgccatggg gacgcgcggt gaattttgac gggcctggaa gcgagggggt
     2941 gcgtgcctat gtcatcgaga atgttcgcat gtggatcagc gattaccata ttgatggatt
     3001 aaggctggat gccgtgcacg cgatctacga tttcggcgcg acgcaccttc tcaaagaaat
     3061 caaacaagcc gctaccgagg ctgccgggga cagaaaattc ccggcgtacg ttgtcgcgga
     3121 aagtgatctg aacgatgtgc gtatcctttt acgggaggat caaggcggct acgagctgga
     3181 tgcgcaatgg agcgatgact ttcatcacac ggtccatgcg ctgttgacgg gagagcggca
     3241 gggctattac tcggactatg gcacagccga acagttgagt gcggtgctag agaaaacgtt
     3301 cgccatcgat ggaggtttta gccgtcatcg aaaccgatgt cacggcgcgc ccgcggcggg
     3361 ccttcccgga gatcgattca tcgggtccat acaaaaccat gatcaggtag gtaatcgagc
     3421 aggtggcgag cggctgagtc atctgctccc accgcctctc cagcggcttg ccgcagcgtt
     3481 cttgctactc gccccacacc tcccgttgct gttcatgggg gaagagtacg gcgaagcacg
     3541 accctttcag tttttttgct ccttcgacgg ggaggagctg atcgagtctg tgcgcaaggg
     3601 gcgacgccag gaatttgaag ccttccatac gagtgacgcc caggtgacgg atccccagga
     3661 cccatctacg tttgaccagt cccgtcttac gtggcggtgg gatgaggtcc cccatcaagc
     3721 gggattgaga cggctctata cggatctact ggaagcccgc cgagtctggc cggccatgcg
     3781 aaattacgcg gagcgcatga gcaggctcca tcgtggagct gacggggcag tggtgttgga
     3841 gttggtgcgg ggagggacca aagcagcgca agcacacacc atccaagcgt tctttaatcc
     3901 tactgagagc gttcagaatc tgccggcccg ggcattacca accttgttgt ttagttcgga
     3961 atgggaacgc tacgatggcg ttcgagcgtc atcttctgag agcggtcagc tgctcccgtt
     4021 cgaatgtgcc atcttcggtc cctctgattg gaaacggtac gcagggcttg agtgaggact
     4081 gggcggcgac agctaactag gtagctaaac atctacgaat ccctgccgag agtcattcag
     4141 agctcgaggc agtcctacgt atatctcgcc gcattcgcac accactcgac gttgctcaat
     4201 gcgagcgtct ctgagccgca gaacagtccc gcgcaccctg cgcaggcggc ccatatcctg
     4261 ttctttcata aaatttaggc tgtcaatcat tgctgacagt gctacagggt tcgccccagt
     4321 tatcaacgaa gcgtccttct cctaggatag gcccacgtga cactccttgt acgtagcgct
     4381 caggccggat acgttcatgt cctcgccagt cgctcgtggc agggctattt caactcatgt
     4441 ggcgttttct aggatagaaa gggactcaat gaatactaca cggcgcactg tgccaggact
     4501 gataagctgc ggactcgttt taggaaccac gttgacatgc cttgcgcaaa caggggcgcc
     4561 ggtgcaggaa caaccaccgg acgtcggtcc acggacgcgc gatcaggcat ctcaagaacg
     4621 agtcgtcaca agggatcgcg cgggtgaccg ggtggtcgtc gatgagcggg tggtggagcg
     4681 ccgcagggaa ggcgaagttt atgtggcagg gtatggaggt ttcacgtggg gacatagctt
     4741 ctcggatctc gaaggccggg gcactatcgc gggtcagaac ttcggaggcc gtgatctggc
     4801 gaattccgga gtgtatggcg cgaagatcgg ctattttcat ccaggtcggt tgaactggct
     4861 cggcatcgag gtggaagggt tcaattcgac gcctcacatc gagcagggcg gagggttgcc
     4921 gggatcacac cttcgggtga caacgctggc gtttaatgtg attgcccgaa cgaaggtcgg
     4981 ttgcagaaat cgcgatgacg tgcgccatcg ggatgaagtc aggcatcgcg atgacggcac
     5041 ggtggtggaa tccgactacg atcggagaca gtggtcgcca ttggacgaaa atctccgctg
     5101 ccccttgcaa ctgtatgccg gtgctggtcc cggaattttc tttgccgaaa cgtcgaatca
     5161 attcggccgc agtactgaca acgggcggat cggcttgaat gcgctggcag gagcgaagta
     5221 tttcatgact cgaaatcttg cggtctttgc agagtacaaa ttcaactatg cgcagttcga
     5281 cttctcgcag atgcagggca cgggagcggg gttcaacggc gaatacaaag cgagccatgt
     5341 ggttggagga ttggcacttc acttctgaca tgattgcggc gtgacgctcc agggttcagc
     5401 ggtcaccgac atgaccgttg agcccgcgcc gcctggctct gcgcgtaact tctcacggtc
     5461 gtcagggacg gggcttcgtg tagaggggaa ggaggtgcca ggtgcgagga tctagcgtgt
     5521 atagacgact tccacgcgac tcaagaaatc tttcagtaac gcgacttgtt tttcgatcaa
     5581 cggtccgttt tgtaccatca cggcttgctc cagatcgtcc ccgatgctgc tgatctgatc
     5641 gaacccgtac ccgccgccgt cgcctttcat ccgatggccc aatatgcgaa tcgtattcaa
     5701 atcgctctgc ttcaggcagg cctcgatcgt cacgagatca cgccggcggt tggcgaggaa
     5761 gcctgggacg atggcttcga gatccggatc gatatggacg atgatcttag gctgtcctgc
     5821 ctcaggttgc ggtggcatca ttgcgcgtgg gtcttctcgt aggcggatag gagttccagc
     5881 agcgtcgtcc gtttgagcgg cttggtcatg tgggcgttac atccggcttc aaaaattctc
     5941 gccgattctt ccttcagcgc cagggcggtc agggcgacga tttggacggc cggaagcccc
     6001 tgttcctgtt cccagcgacg gatcgtgcgt gtggcagtca gtccgtccat caccggcatc
     6061 tgcatatcca tcaaaatcag gtcatagtga ccgttttgaa atttggcnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnngta tccgcccagt cccaagtcgt aggtcttcgc
     6241 gatgtcatcg gcccatcggt cggaggccag catgatgatc gtcaagttgc gaaggcgggg
     6301 atcggcatgg atacgttcga tgacctcgaa tccatccatt cccggcatgc ggcagtccaa
     6361 caaaaggaga tcgaaggacg tctgcgaggt gagcgcgctt tcaagcacct ccatggcctg
     6421 ctctccgctc tcggcttcca ccacggtggc gccccaggca aagagcgttt cccgcagaat
     6481 caaacggttt gtcgggtaat catccacgac gagacagcgc acaccgctca ggttcagggc
     6541 ccccgcgcct ttcgaaggtg ccggcgcagc ttgtacgccc aaccgaacga ggcaatggaa
     6601 cgtgctgccc ttgccgaggg tgctctcgac ccaaatcttt ccgcccatga gtgcggccag
     6661 ttgctgtgaa atggataatc ccagcccggt gcccccatag cggcgggcga tcgtgctgtg
     6721 cgcctgcgta aaggtgtcga agatggctgt cattttgtcg tggggaaccc cgatgcccgt
     6781 atcagtcacc gagaatcgga tggcgccggg tgtctggcag tccggatcgt tggtgatggt
     6841 catgaccacc gatcccttct ccgtgaactt gatcgcgttt cccagcaggt tgagcagaat
     6901 ctggtagagg cggttgggat caccgatgag gtggcacggc acatcgttcg acaggtgaca
     6961 gaccagttcg aggccttttt cgttggcgcg gagcgccagc atctcgctcg ctttgtcgat
     7021 gagttccgag aggtcgaagt ctatgctatc gagatcgagc cggccggatt caacttttga
     7081 caaatcgaga atgtcgttga tcagactcag caggcttgcg cccgcgcgtc gaaagatccg
     7141 gagatacttc cgttgctccg gggtcaaagt cgtatcccat aacagatcag ccatgccgat
     7201 gatggcgttc atcggcgtcc tgatttcatg actcatgctg gccaggaaat cactctttgc
     7261 gcggttggcc acgtcggccg catctttggc gcgttgcagc tcctcaacgc ggcgttgcaa
     7321 ttccttagag gcgcgatcaa gcgcgcgttc ggcacgccgg cgcgcggctt gttcacgttg
     7381 gagtgctgtc tgtggacggg aactggtgcg aagggtgcgt ttaggagtgg tgcttgtggt
     7441 gcgtctggtt tgcgagatgc gtttcgccat gtcgtcgtcc ggtcgccttc agatggtcat
     7501 aaccatatcg tatcgcgagt agaggaagcc ggcacgatcg tcgcgacggc gagtaactgt
     7561 cagtccttat gcgtatggtg gacgagggac agcacacccg cttgtttcgc gcggttgcgc
     7621 ccttcctgct tggcttgata gagcgcctgg tcggccgcgc ggatcagatc ggtcggggcg
     7681 gcgacgcggc tgggcacggt gctggcgaag cccaggctga tcgtgaccgg atcgccgttc
     7741 ggatgtttca tctctgcttc gcagactttc cgtctgaggg tatcggcgac ttgtgcggct
     7801 ccgtcgacag ttgtgccggg cagcacgatg acgaattcgt cgccgccgta ccgtgcgacc
     7861 aagtcgccgg gacgattgac gtggctggag atcaactggg cgacctgacg gaggcattcg
     7921 tctccggcct gatggccatg gctgtcgttg taggttttga aaaaatcgat atccagcatg
     7981 atgagcgaca gcggggtgga ttcgcgcgcg gcgcgccgcc attcctggtc gaggaagtca
     8041 tcgaactgcc ggcgattggt gatgccggta agcccgtcaa ggcaggagag gcgcagcagc
     8101 atctggttgg cttcctgcaa ctggcgcatg acctcgagca attcctgctc gcgtgcccgg
     8161 cgccgttcga tttcatggac gaggcggagc acgcaacgga cgcgggtcaa gagttcaatc
     8221 ttgctgatcg gcttcgccac ataatcaatg gcgcctgcag caaaggcgag ttgcagatcg
     8281 acggggtcgg tcttgacggt gaccatgatg atcgggatgt cccggtatcg ctcgacggcc
     8341 ttgatctggc ggcaggcttc gatgccattg gtggacggca tgacgatatc catcaggatc
     8401 aaatccacac ggccggccat ctggcgcatc ccgtcgaggc ccagcaaacg aaacgcggcg
     8461 gcggcggact ctgccacaaa cgtctcttcg tatccggcat tggcaaggat ggattggatc
     8521 aacagacgat cgtctgtcga atcatcgacg atcaggatac
//
LOCUS       DAOQEW010000165         8462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2843_length_8462_cov_7.238254, whole genome shotgun sequence.
ACCESSION   DAOQEW010000165 DAOQEW010000000
VERSION     DAOQEW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_8462_cov_7.238254"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..953)
                     /locus_tag="PLS35_15155"
     CDS             complement(195..953)
                     /locus_tag="PLS35_15155"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNI20060.1"
                     /translation="MSFYEQVRSDVLRHGAINNSYLDRFLAGDVSDKEFREFAVEFYN
                     FSRFFPRILAAQLVNTEDEAVADELTKVLYSELGDGSVKHRHELLYRNFLRSLGIDIH
                     VAMTTPMRPSTKAYIEGMERLYGSGNHAMALGASFGLENMAITMWDHLIPGLTRLRAE
                     RYADMDMTYFVFHRQLESEHERAMHHAIVAVDGDSGAPHARMSDKEKDDFRLGMNAVL
                     DYLEGFWMGLERKPAGVAQSRSRPVHGLESELRG"
     gene            1168..1767
                     /locus_tag="PLS35_15160"
     CDS             1168..1767
                     /locus_tag="PLS35_15160"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI20061.1"
                     /translation="MKEAIGVDHITLCVKNLSATKYLFTHILGFDVIWSAQDVGSEKS
                     SMDTVVVQRGAAKIALMQGRNKERKSQICEFVETYGEGVQHIALEVDDIEAVCREWEH
                     HGVRFSGDIKDGRDGFGPLRQRFTYPLFPGCGLFLELTQRQHGTEESKTFVRATVESL
                     YRDIERDQIKGVARTIIDYETLPLPFEEEVEAASLRHAS"
     gene            1806..2921
                     /locus_tag="PLS35_15165"
     CDS             1806..2921
                     /locus_tag="PLS35_15165"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNI20062.1"
                     /translation="MYLVKKGTAPNQAHVGIPEGLHEEEHGRRGFSGPSSHLYHTHPP
                     TAWSRIEGPLRPRAFDCTELPTPDHYATDRRALLILQSPDARVSVSRRGTTMQHFVRN
                     ADGDEIVFVHSGQGTWETDYGLLRYEPGDYLVIPKGTTYRIHVDHQEQPGLFLIIETP
                     NPVELPDRGPLGRHALFDPGVIVTPEPASPPDDANGRREWEVRIKRDQEETRVYYPFY
                     PMDVAGWKGDLWVAKLNVRDFRPVTSPRYHLPPSVHATFQAGGLLISTFAPRPLETDP
                     DALRVPFYHRNMDYDEVLFYHRGEFFSRAGIQAGMLTLHPQGIHHGPQPQAIAASKAK
                     EQTNEVAVMIESRHAFQVRPELEGVELKDYAMSWSQP"
     gene            2918..3919
                     /locus_tag="PLS35_15170"
     CDS             2918..3919
                     /locus_tag="PLS35_15170"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNI20063.1"
                     /translation="MKLVSFEVHTPVGVFRRIGAMRAGSIVDLNMAYARLLAEQKESR
                     PRRLADAQVPATMLEFLQGGASAMDAARHAFDFVAQDASLKGPSGETIIYNAADVHII
                     APLPNPASLRDFIAFEDHIAATSKRRGQPIPPEWYKAPVYYKGNHRTIVGPDYSLPWP
                     LNTQKLDYELELACIIGRDGIDVPERDAAQYIAGYTIMNDLSARDIQFQEMACRLGPA
                     KGKDFATAIGPCIVTPDEIPDLASLQMIARVNGEVWSQGRFGSIHWSFPQMIEHVSRG
                     EAIYPGDLFGSGTVGGGCGLELDRYLQPGDCIELEIQPIGILKTTIAGHNKRSREEA"
     gene            3916..4677
                     /locus_tag="PLS35_15175"
     CDS             3916..4677
                     /locus_tag="PLS35_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20064.1"
                     /translation="MMPVVTKSSEAAIVETVERQRAMYDGSDIDVRMQHLIRDTPHPT
                     LSSWDIQRLLRASRAVYFDSHVEVVSGHHTEVYLRFESIGRFPDLVTIIASNMAEWIL
                     QTFHHDPPAGILTTSSDARLLAKRTCEILATHLPLRTVLTPFNPDTGKIGTEIISGTV
                     APGERFLSLNDVTTRGMCVSKLGKVVTDHGGQLAGMMVFARRDSGQFPFMDELTATYP
                     FYFGVDLDMPQWEPADCHACRDGKPLVSWRDLPAL"
     gene            4714..5715
                     /locus_tag="PLS35_15180"
     CDS             4714..5715
                     /locus_tag="PLS35_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNI20065.1"
                     /translation="MDQAGVAQDIKRSDLLEMYFYLRLTRSLEDRITALYRQGRIVGG
                     VYTSHGMEAIAVGYASALQSDDVIAPFHRDMGAFLIRGFSPGEIIAQYLGKQAGPTKG
                     KDGNVHMGDLKRGVIGFVSHLADNMPVAAGAALAFKIRGESRVAFAGTGDGGTSRGDF
                     HEAMNFAAVRKLPVVFFCTNNQYAYSTPVRYQMAITDVVERAKAYGMPGEIVDGNDVA
                     AVYLASRRAIAKARSGEGPTFLEFKTMRMHGHSEHDAATYVPRTLLDDWKTKDPILRA
                     EQLLMQLGYGDDSYFHEVGERAKKDVDAGTEFAEQSPLPEGRETLVGVFATQDEVLP"
     gene            5712..6710
                     /locus_tag="PLS35_15185"
     CDS             5712..6710
                     /locus_tag="PLS35_15185"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNI20066.1"
                     /translation="MTTATATQETHTTYVDAISQALDEEMSRDERVFLMGEDIGAYGG
                     AFKVTEGFLKKYGEWRVLDTPLSESGFVGAAIGAAMMGLRPVVEMQFADFISCAFDQI
                     TEVAAKNHYRWGAAVPLVIRAPFGGGVHGGPFHSECPEGWFFHSPGLKIVAPSTPYDA
                     KGLLIAAIRDPNPVLYFEHKFLYRRIKATLPQEEYIIPLGKADVKRSGRDMSLITYGA
                     MVHLALEAAEVLQQEGIELEVVDLRTLIPLDKETLYASVRKTSKAIILHEDNKTGGIG
                     AEISALLTEDCFDCLDGPILRIAPPDTPVPFSTPLEEFFLPKVSDIVAGARKLAAY"
     gene            6752..7921
                     /locus_tag="PLS35_15190"
     CDS             6752..7921
                     /locus_tag="PLS35_15190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNI20067.1"
                     /translation="MATDIVMPQLGESIAEGTVVKWLIQAGGAVEKDQPLLEVETEKV
                     ALDVPSPAAGFLAEVMVQEGTTVPVGTLLARLETQPAAAGVVNRVGDVGVRARQEQQP
                     GEQHYSPAVKQLAREHGVDLTVVPGTGEGGRVTKKDLLDYVSTRQQPSGEPPRTAQAP
                     TVVPQPRETPEEIIPFTQMRKTIADRMLLSRRTSAHVTTFFEADFRGIDIFRTGRTLT
                     YLPFVISAVTRAMKDLPLLNASWREQGLAIKKAVHIGIAVALDEGLLVPVIRHADQKG
                     LTQLANEVAELAERARNKRLTPDDVLDGTFTITNHGGFGSLFSTPIIHQPQTAILGVG
                     SVQKRAVVIDDAIAIRPMCYLSLSFDHRIIDGATADRFMAKVKQHLQQSDWEKLL"
     gene            7918..>8462
                     /locus_tag="PLS35_15195"
     CDS             7918..>8462
                     /locus_tag="PLS35_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNI20068.1"
                     /translation="MTARHHAVIVSAVRTPMGSFNGGFSSIPSTKLGSIAIAEALQRI
                     QLTGEQIDEVLMGCVLSAGLGQAPARQAAIGAGLPHRVGAVTVNKVCGSSLQTVIMAA
                     RAIALGEAQIIIAGGMENMTRAPFLLEKARQGYRLGHGELTDSLIKDGLWDVYNQFHM
                     GNAGELCAARYQFSRQQVDDFA"
BASE COUNT         1835 a         2522 c         2475 g         1630 t
ORIGIN      
        1 gttcaactcc tggaaggaaa tacgtgtgcc gtagaagaag agggcgggcg attcaggaaa
       61 ccgggacgcg gagcgccgaa gcagatccgg aactgtccac tccggatagt cacaggtagc
      121 ggagacacca ggatcatatc ggctgatcca aggccgcggc atctggtcct cccatccctg
      181 accgatcagg gaggttaccc gcgtagctca ctctccagcc cgtgcaccgg tcgtgaccga
      241 gactgggcca caccggccgg cttccgttcg agtcccatcc aaaatccttc aagatagtcc
      301 agtacggcat tcatcccaag acggaagtcg tctttctcct tgtcagacat gcgagcgtga
      361 ggagcgccac tgtcaccatc gacggcgaca atggcatggt gcatcgctcg ttcatgttca
      421 gattcgagtt gccgatgaaa gacgaaatac gtcatgtcca tgtcggcata gcgctcggcg
      481 cgcaaacgcg tcagcccggg aatgagatgg tcccacatgg taatcgccat attctccaga
      541 ccaaatgacg cgcccaatgc catcgcatgg tttccactcc cgtaaagacg ctccattcct
      601 tcgatatagg ccttggtgga cggccgcatc ggcgtggtca tcgcgacgtg aatgtcgatg
      661 cccagcgatc gcaggaagtt tcgatacagc aattcgtgcc gatgtttgac ggagccgtcc
      721 cccagctcgg aatagaggac cttcgtcaac tcatcggcga cggcttcatc ttcggtgttg
      781 actaattggg cagcgaggat acgggggaag aagcgcgaga agttataaaa ttcgacggcg
      841 aactcacgaa attctttatc gctcacatcg ccggcgagaa aacgatcgag gtaggaatta
      901 ttaatcgcgc catgtcgcag cacgtccgag cgcacttgtt cgtaaaacga catcgtgcac
      961 ctccgccgtt gtcccggtac gccgtttcat tgcctgctcg cgcgcgattg gttccgaact
     1021 atcatgcatc atgatcaaat aatacaaaat ctatattgaa tgatgcaact cttatgttgt
     1081 agagtgtgct catgcttcac ggaataagat tttctgacac gcggcatcgg ctgagattcc
     1141 ccgtgactac acctgggagg aaccgccatg aaagaagcca tcggggttga tcacatcacc
     1201 ttgtgcgtga agaatctctc ggccacgaaa tatctcttca ctcatattct tggctttgac
     1261 gtcatctggt ccgcccagga tgtaggcagc gaaaaatcca gcatggatac ggtcgtcgtc
     1321 cagcggggcg cggcgaaaat tgccttgatg cagggcagaa ataaggagcg caaatctcag
     1381 atctgtgaat tcgtcgagac ctatggcgaa ggcgtgcagc atatcgccct cgaggtcgat
     1441 gatatcgagg cggtctgccg cgaatgggaa catcatggcg tccggttcag cggagacatc
     1501 aaggatggac gcgacgggtt cggccctctc cggcaacgct tcacctaccc gttattcccc
     1561 ggctgcggat tgtttctcga actgacacag cggcagcatg gcaccgaaga atccaaaacg
     1621 ttcgtccgcg cgacggtcga gtcgttgtac cgggacatcg aacgcgatca gatcaaagga
     1681 gtggcacgga ccatcatcga ttacgaaacg cttccgcttc cgtttgaaga agaagtcgaa
     1741 gctgcatcgt tgcgacacgc atcgtaaaca catcgagccg cacctgacct gtcaggaggt
     1801 ttccgatgta tctggtgaaa aaaggcaccg ctccgaatca ggcccatgtg ggaatccccg
     1861 aaggcctgca tgaggaggag catggtcgcc ggggcttcag tgggccctcg tctcacctct
     1921 atcacacaca tccgcccact gcttggagcc gcatcgaagg tcctctgcgg ccgcgcgcat
     1981 tcgattgcac ggaattaccg acgcctgatc actacgcgac ggaccggcga gcactcctga
     2041 tcttacagag cccggatgcc cgcgtgtctg tttctcgacg cgggaccacc atgcagcatt
     2101 tcgtgcgcaa tgccgacggg gacgagatcg tgttcgtcca cagcgggcag ggaacctggg
     2161 aaaccgatta cggcctgttg cgctacgaac cgggcgatta tctggtcatt ccgaaaggca
     2221 cgacgtatcg catccatgtg gatcatcagg aacagccagg tctgtttctg atcattgaaa
     2281 caccgaatcc tgtggagctt ccagaccgag gacctctcgg ccggcatgcc ttgttcgacc
     2341 cgggggtcat cgtcacgcct gaacccgcgt cccctccgga tgatgcgaat ggccggcgtg
     2401 aatgggaagt gcgtatcaag cgcgatcagg aagagacgcg cgtctattat ccgttttatc
     2461 ccatggatgt cgcagggtgg aaaggcgatc tgtgggtggc caagctgaac gtccgggatt
     2521 ttcgcccggt cacgagccct cgctaccatc ttccacccag cgtccacgcg acgtttcaag
     2581 ccgggggact cttgatttcc acctttgcgc caagaccgct ggaaaccgat ccagacgcat
     2641 tacgtgtccc cttctatcat cgcaacatgg actacgacga agtcctcttc tatcaccggg
     2701 gggagttctt cagccgtgcc ggcattcagg ccggcatgtt gacgctccac cctcagggca
     2761 ttcaccacgg accacagccg caggccatcg ctgccagcaa ggcaaaggaa cagaccaatg
     2821 aagttgccgt gatgatcgaa tcacgccacg cctttcaggt caggccggaa ttggaaggcg
     2881 ttgaactgaa ggattacgcc atgagttgga gccagccatg aaactggtca gtttcgaggt
     2941 tcacaccccg gtcggagtct ttcgcaggat cggcgcgatg cgggcaggat caattgtcga
     3001 tctcaacatg gcctatgccc gactgctcgc tgagcagaag gaatcgcggc cgcgccgtct
     3061 cgccgatgcg caagtcccgg ccacgatgct ggagtttcta caaggcggcg cctcggccat
     3121 ggatgcggcc cggcatgcgt ttgatttcgt ggcacaggat gcctccctca agggcccatc
     3181 cggtgaaacg atcatctaca acgcggccga tgttcatatc atcgcgcctc tgcccaaccc
     3241 cgcctcgctc cgcgatttca tcgccttcga agaccacatt gccgccacgt cgaaacggcg
     3301 aggccaaccg attcctcccg aatggtacaa ggcgcccgtg tactacaagg gtaaccaccg
     3361 gaccatcgtg ggcccggatt actcgctccc ctggccgtta aacactcaga agctcgatta
     3421 cgaattggag ctggcctgca tcattggacg cgacggcatc gatgtgccgg aacgcgacgc
     3481 ggcgcaatac attgccggct acacgatcat gaacgacttg agcgcgagag atatccagtt
     3541 ccaggaaatg gcctgccggc tcggtcctgc gaagggaaaa gacttcgcca ccgccatcgg
     3601 cccttgcatc gtgacaccgg acgaaatccc ggatcttgct tcgctgcaga tgatcgcccg
     3661 tgtcaatgga gaagtctggt cgcagggccg cttcggttcg attcattggt cattccctca
     3721 gatgatcgag catgtctcgc ggggggaggc catctatccc ggcgaccttt tcggctcggg
     3781 aacggtcggc ggcggctgcg gccttgaact cgaccggtac ttacagccgg gagactgcat
     3841 tgaattggaa atccaaccta tcggcattct gaaaacgacg atcgcgggac acaacaagcg
     3901 ctcgcgcgag gaggcatgat gccagttgtc acgaaatctt cggaagccgc gatcgtggag
     3961 accgttgaac gacagcgggc gatgtatgat ggatcggaca tcgacgtgag gatgcagcac
     4021 ctcattcgcg acaccccgca tcccaccttg tcgtcctggg atattcaacg cctgctgcgt
     4081 gcctcgcgcg cggtgtattt cgacagccat gtggaggtcg tgtccggtca ccataccgag
     4141 gtctacctgc gattcgaatc catcggccgt tttcccgacc tcgtgacgat catcgcgtcc
     4201 aacatggctg agtggattct tcaaacattt caccatgacc cgccagccgg aattcttacc
     4261 acgtcgtctg acgcgcggct gttggcgaaa cgaacctgcg agatcctggc gacgcatctt
     4321 cccttacgca ccgtgctgac gccttttaac cccgacacgg gcaagatcgg cacggagatc
     4381 atcagcggaa cggtggcccc cggggagcgg ttcctttcac tcaacgatgt caccacacgc
     4441 ggcatgtgcg tcagcaagtt gggaaaggtc gtcaccgatc acggcgggca gcttgcgggc
     4501 atgatggtgt ttgcgaggcg ggacagcgga cagtttccgt tcatggatga gctgacggcc
     4561 acctatccgt tttacttcgg cgtggatctg gacatgccgc agtgggaacc ggccgactgc
     4621 cacgcctgcc gggacgggaa accgttggtg tcctggaggg acctgcccgc gctctgacgt
     4681 gatgcggata gccgatcgat gaaggaggca gctatggacc aggccggcgt cgcacaggac
     4741 atcaaacgca gcgacctcct tgagatgtac ttctacctgc ggctcaccag aagcctggag
     4801 gaccggatca cggccctcta tcggcaaggc cggatcgtcg gtggcgtcta caccagccac
     4861 ggcatggaag ccatcgccgt cggctatgcg tccgccttac agtccgacga tgtcatcgcc
     4921 cccttccatc gcgatatggg agcgttcctg attcgcgggt tttcccccgg agaaattatc
     4981 gcccaatacc tcggcaaaca ggccgggccc acaaaaggca aagacggcaa cgtgcacatg
     5041 ggcgatctga aacgcggagt gatcggcttc gtcagccacc tggccgacaa catgccggtc
     5101 gcggccggcg cggcactggc gttcaaaatc cgtggtgaat cccgcgtggc cttcgcagga
     5161 accggcgacg gtggcacgag ccgtggcgac ttccatgaag ccatgaactt cgcagcggtg
     5221 cgcaaactgc cggtcgtctt cttctgcacc aacaatcagt atgcctactc cacgccggtc
     5281 cgttaccaaa tggcgatcac cgatgtggtg gaacgagcca aggcgtatgg tatgccgggc
     5341 gaaatcgttg acggcaacga cgtggccgcc gtctacctgg cgtcccgtcg agccattgcg
     5401 aaggcgcggt ccggcgaagg tccgacattt ctcgaattca aaaccatgcg catgcacggc
     5461 cattccgaac atgacgcggc cacgtacgtc ccgcgtacgc tgctggatga ttggaagacc
     5521 aaagacccga ttctgcgcgc cgagcagttg ctcatgcagc tgggctacgg ggatgactcc
     5581 tactttcatg aggtcgggga acgcgccaag aaagacgttg atgcaggcac cgagttcgcc
     5641 gagcaaagcc cgctgccgga aggacgtgag acgcttgtcg gcgtgttcgc cacccaagac
     5701 gaggtgttgc catgacgacc gcgaccgcca cgcaggagac gcataccacc tacgtcgatg
     5761 ccatatcaca ggccctggac gaagaaatga gccgcgacga acgagtgttt ctcatgggtg
     5821 aggacatcgg cgcctacggc ggcgcattca aagtcacgga agggtttctc aagaaatatg
     5881 gcgaatggcg agtcttggat acacccctct ccgagtccgg cttcgtaggc gccgccatcg
     5941 gcgcagccat gatgggattg cgccccgtgg tcgaaatgca gttcgccgac ttcatttcct
     6001 gcgccttcga tcagatcacc gaagtggccg ccaaaaatca ctaccgctgg ggagcggcag
     6061 tccctctagt gatccgggcg ccattcggcg ggggcgtcca tggaggtccg tttcattctg
     6121 aatgtccgga aggctggttc tttcattcgc ccggcctcaa aatcgttgcg ccctcgacgc
     6181 cctatgacgc caaaggtctc ttgatagccg ccatccgcga tccgaacccg gtcctctact
     6241 tcgaacataa gtttttgtat cgacggatca aggcgacgct gccgcaggaa gagtacatca
     6301 ttcctctcgg caaggctgac gtgaaacggt ccggccgcga catgtcgctg attacttatg
     6361 gagcgatggt gcatttggcc ttggaggcgg cggaagtgct gcagcaagaa ggaatcgagc
     6421 tggaagtggt ggatcttcgc acgctgattc ctctcgacaa agagacgctc tacgcctccg
     6481 ttcgcaaaac cagcaaggcg atcattctcc atgaagacaa caagaccggc ggcatcgggg
     6541 ccgaaatttc cgcgctgctg accgaagact gtttcgactg tctggacggg ccgattcttc
     6601 gcatcgcccc gcctgacacg ccggtgccgt tcagcacacc attggaagaa ttctttttgc
     6661 ccaaggtcag cgacatcgtc gcgggagcca ggaaactcgc ggcctattga tgggatggac
     6721 taggtaaaca ggcatgcagg agacgtgatc cgtggctacc gacatcgtca tgccgcagct
     6781 cggtgaaagc atcgctgaag gtaccgtcgt gaagtggctg atccaagccg gcggtgcagt
     6841 cgaaaaagac caacccctcc tggaggtgga aaccgaaaag gtggcgctcg atgtgccgtc
     6901 tccggccgcg gggttcctcg cggaggtgat ggttcaagag ggcacgacgg tgccggtggg
     6961 caccttactc gccaggctcg agactcaacc ggctgcagcg ggggtcgtca accgggtcgg
     7021 agacgtgggt gtgcgcgcaa gacaggagca acagccgggc gaacagcact attcgccggc
     7081 tgtcaaacag ctggcacgag agcatggggt ggacctgacc gttgtccccg gcacagggga
     7141 aggcggccgg gtgaccaaga aagacctgct ggattatgtc tccacgcgtc aacagccaag
     7201 tggcgagcca ccacgaacgg ctcaggctcc aaccgtcgtc ccgcagcccc gcgaaacgcc
     7261 ggaggaaatc attccgttca cccaaatgcg aaagaccatt gccgaccgca tgctcctgag
     7321 ccggcgcacc tccgcccatg tcaccacctt tttcgaagcg gacttccggg gcatcgacat
     7381 attccgcacg ggacgcacac tcacctacct gcccttcgtc atcagcgccg tcactcgggc
     7441 catgaaggac ctgccgttgc tgaatgcctc ctggcgggag caaggcctcg cgatcaaaaa
     7501 agctgtccac atcggcattg ccgtcgcgct cgacgaaggg ctactggtgc cggtgattcg
     7561 ccatgccgat cagaaagggc tcacccagtt ggcgaacgag gtcgccgagt tggcggaacg
     7621 ggccaggaac aaacggctga cgccggatga cgtgctcgac ggcacgttta cgatcaccaa
     7681 tcacggcgga ttcggcagcc tgttcagtac gccgatcatt catcagccac agacggcgat
     7741 tctcggcgtg ggatcggtcc aaaaacgcgc cgtggtgatc gacgacgcca tcgccatccg
     7801 ccccatgtgt tatctgagtc tctccttcga ccatcgcatc atcgacgggg cgacggcgga
     7861 tcgattcatg gccaaggtga agcagcatct ccaacagagc gattgggaga aacttctgtg
     7921 actgccagac accatgccgt catcgtcagc gcggttcgaa cgccgatggg aagcttcaac
     7981 ggcgggttca gctcgatccc ctcaactaaa ttgggaagta ttgccatcgc cgaggcgctg
     8041 caacgcattc agctgacggg cgaacagatc gacgaggtgc tgatgggatg cgtgctcagc
     8101 gccgggctcg gacaggcacc cgccaggcaa gcggcgatcg gagcaggact gccgcaccgg
     8161 gtcggggctg tgaccgtgaa caaggtctgc gggtccagct tgcagacagt catcatggca
     8221 gccagggcca ttgccttggg tgaagcgcaa atcatcattg caggcggaat ggaaaacatg
     8281 acccgggccc cctttctgct ggagaaagcc agacagggct atcggcttgg acatggagag
     8341 ctgacggaca gcctgatcaa ggacgggctc tgggatgtgt acaaccaatt tcacatgggc
     8401 aacgccggag aactctgcgc cgccagatat cagttttcac ggcagcaggt cgacgacttt
     8461 gc
//
LOCUS       DAOQEW010000166         8379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2902_length_8379_cov_7.979337, whole genome shotgun sequence.
ACCESSION   DAOQEW010000166 DAOQEW010000000
VERSION     DAOQEW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_8379_cov_7.979337"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /gene="pstA"
                     /locus_tag="PLS35_15200"
     CDS             complement(<1..1634)
                     /gene="pstA"
                     /locus_tag="PLS35_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNI20069.1"
                     /translation="MGKPSLRMKEFWRGGELFVWMTASGVAISLLMVAGMLVLIMFNG
                     LGQFWPGTLQQVTLKTQETVIGHLVGEETIPHSMTTDALLGQRRFRYRVGNRDQFGSE
                     YRWINESEIASISAPDDLVFVERREWGPAYGRISLAAESTAATNADGSAWSTIETLVD
                     RANQLRQRIEHLEREDIGSINYRIEKARLARQTLVLKGAHNPASIEADRRLVEQIAAL
                     EREYLSKTDALEQLHKEATQEVVLLTLSTGRSVRLSLDQVLAVSRPNAMNGFEKAVAY
                     VRNLWHFVSGEPREANTEGGIFPAIFGTVLMVFLMTLAVMPFGVMAAVYLREYAKQGP
                     LVRLVRIAVNNLAGVPSIVFGVFGLAFFVYALGGTIDQILFPESLPNPTFGTGGILWA
                     SLTLALLTVPVVIVATEEGLSAVPRDFREGSVGLGATKFETIRHVILPCALPGILTGL
                     ILAMARAAGEVAPLMLTGVVKLAPALPLDEYLPFLHLERKFMHLGFHIYDVGFQSPNV
                     EAAKPMVYMTTLILILVVVLLNLAAVMLRNRLRKRFA"
     gene            complement(1627..3855)
                     /locus_tag="PLS35_15205"
     CDS             complement(1627..3855)
                     /locus_tag="PLS35_15205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNI20070.1"
                     /translation="MITNPLSYLSGPVHLRSVIDRLARGIITVGGLATIVSILGIFFF
                     LFREVTPLFTAPSAKLSQRLSVPALLADEGPAQVAVDEHREIAQVFTSGAIQFFDLAS
                     GQPIALEMPAQLTSRQVTAMASGGGPTARLAVGTAAGEILLLKIGTTTEFSDQGERRK
                     RPHIRAGAPIPVTESPIVRLAYRSNDQGSLLALATKTGRLFLARIAPDDPTGAGVMVT
                     ELPQPSGGVASLALEASLDHLYVGTTDGHIIHVGVSETQPPDVRASYAVAGPTTAISM
                     LGFLSGDRSLVVMAADGAVSTWGLVRRSDVPTGWTLTQMHTFRSHAAPVTAFAPSQRV
                     KGFVTGDTKGNVFLHHATSSQTLLRVANGEFPIRAVAFAPKGDGVIALDGAGQLSLYQ
                     VQNPYPEVTLDTLFGKVWYEGYGQPAYVWQSSSGSDEVEPKLGLLPLAFGTLKGTIYA
                     LFLAVPIAIFAAICTSQFMHHDLRAKVKPIIEVMAALPTVVLGFLAGLWLAPLLERML
                     PALAGMVLVLPVLVIVASFSWHLCPMSIKRHFSPGNEALLLMPILALGIGGCLWAHSF
                     FEVWWFDGNIKTWLSSRLGIQYDQRNALVVGIVMGFAIVPVIYSIAEEALSNVPKSQI
                     AGSLALGATRWQTVLHLVLLSASPGIFSGVMIGFGRAIGETMIVLMATGNTPILDWNW
                     ANGFRTLSANIAVEIPEAPHGGSLYRVLFLAALLLFIVTFVINSLAELVRQRLRDKYS
                     HG"
     gene            complement(3855..4865)
                     /locus_tag="PLS35_15210"
     CDS             complement(3855..4865)
                     /locus_tag="PLS35_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI20071.1"
                     /translation="MTVSRQICLSLTLTALGPFMTMCLVSAAPGDTPAVETLTVDAGI
                     KPYHKVSGVSGTINSIGSDTLNNLITLWAEGFRKQYPNVKIQVEGKGSSTAPPALIEN
                     TAQLGPMSRTMKSSEIDAFESKFGYPPTAYPVAVDALALFVNKDNPVAGLTIAEVDAL
                     FSKSRRQGHTTDLTRWGQLGQNGDWANAPVSLYGRNSASGTYGFFKDHALKNGDFKDQ
                     VKEQPGSASVVQGISEDRYGIGYSGIGYSTSGVKMVPLAAKAGQPYIEPTQANTKNGT
                     YPLWRYLYIYVNQAPGKPLSPIVKEFLAYVYSKEGQSDVLRDGYFPVDAKLAEQQLGK
                     IR"
     gene            5080..5772
                     /locus_tag="PLS35_15215"
     CDS             5080..5772
                     /locus_tag="PLS35_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20072.1"
                     /translation="MRPSTVHIVEDEPLHAGLLDRALRLAGFDTLLSSDGASGWQEIQ
                     RRLPTLILLDLMLPALSGQEICRMVRQHSATRHIPIIMLTAVGGEPERIAGLDMGADD
                     YVVKPFSPREVVSRVRTVLRRGAHSADGVAPIVNAAVRMQGPYFAVLLGERELLLSRK
                     ETALLQTLLGRENALVAAEELLTLPEGERPGLLLQELDYDVRGLRRKLENSGAGSVDM
                     LPGFRYRFRSRL"
     gene            complement(5809..6144)
                     /locus_tag="PLS35_15220"
     CDS             complement(5809..6144)
                     /locus_tag="PLS35_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20073.1"
                     /translation="MLHSQLHVIQQSAGCQGQRVEGLGTFQPGGSTMVHQSAVLSSEC
                     RVLELLHGRKSLTLEQVVTLLPELSWNQVFKTIDELSRHGKIILLRRGFDYEIERISS
                     RQPALSCFG"
     gene            complement(6209..6808)
                     /locus_tag="PLS35_15225"
     CDS             complement(6209..6808)
                     /locus_tag="PLS35_15225"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNI20074.1"
                     /translation="MKVMVHGDPGTDLSSTLLNHDRVIHQEVSQMTFTSFPVRVPRVA
                     DLAGRAIETDLTDLWMKGIKAVLSLLILTILIALTGGVIKTFLDIKLLLEAPVEVGLR
                     QIIVDTLILLAVVEVFKTTLTYFSEGRVKVTFIVDTILVVMLTEIISLWFKEADQTKL
                     LSLGAIVLALGAVRVVAVRCSPAQGDGNGKCAGSCREGI"
     gene            6980..7690
                     /locus_tag="PLS35_15230"
     CDS             6980..7690
                     /locus_tag="PLS35_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20075.1"
                     /translation="MQHATEPLRHHGIPVLLVTKDEEFAMSVHQLLRPNGYHVSLAPT
                     AEAALSLASGASFKLALIDRRHKVVGTLRQNLAFRHIPMIALQPLGEPCTEEETVEDL
                     AAGFDIVIDSHRHREWLAIIKALLRRQEIQAAPLREFRVNTLCMNLDRHEVRVEGKSI
                     QLTPKEFQILRVLLQHPGRVLTRQELLNLVWGEDYALEEHALDVHIHALRHKLETKPS
                     APQWILTVRGVGYKLSTD"
     gene            7845..>8379
                     /locus_tag="PLS35_15235"
     CDS             7845..>8379
                     /locus_tag="PLS35_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20076.1"
                     /translation="MEMRPLVASISGLVGSAMLGLCVTASWADDSSRQGQSSHTVVAQ
                     AMGPAPNGTSSPPPAPDMPLGGTSIEDLLMQKGTITKEEWIQIRAEQEYKAGERDKRV
                     DSLDEWKSKAELLPILRDKVNFGLNALQWLYTHQDAHVPEGRSQDNLSIRRSEILFWG
                     RISDTLPRWHTLMEFQSI"
BASE COUNT         1920 a         2481 c         2325 g         1653 t
ORIGIN      
        1 gcgaatcgct tgcgtaagcg gttgcgcaac atcaccgcag ccagattcaa cagcaccacc
       61 acaagaatca gaatgagggt ggtcatgtag accatgggct tggccgcttc gacattcggc
      121 gactgaaacc ccacgtcata aatatgaaaa cccaagtgca tgaatttgcg ttcgagatgc
      181 aggaagggaa gatactcgtc cagcggaagc gccggggcca gcttcaccac gccggtaagc
      241 atcagcggcg ccacttcgcc tgcggctctg gccatagcga gaatcaatcc cgtcaaaatt
      301 ccgggcaagg cgcaaggcag aatgacatgc cggatcgtct caaatttggt ggcccccaac
      361 cccacggatc cttcccgaaa atcgcgcggc accgcggaga ggccttcttc cgtggcgacg
      421 atcacgacag gaacggtgag cagtgcgaga gtcagcgaag cccagagaat cccgcccgtc
      481 ccgaacgtgg gattcggcaa ggattcagga aagaggatct gatcgatagt tccccccagc
      541 gcatagacaa agaaggccaa cccgaacacg ccgaacacaa tcgagggcac tccggccagg
      601 ttgttcaccg caattctgac cagcctgacg agcggtcctt gtttcgcata ttctcgaaga
      661 tacacggccg ccatcacccc gaaaggcatc acggccaagg tcatcaaaaa caccatcagc
      721 accgtcccga agatggcagg aaagatcccg ccctccgtat tggcttcgcg aggttcgccg
      781 gagacaaaat gccaaaggtt ccggacatag gccacagcct tttcgaaccc attcatcgca
      841 ttcggccggc tgacagccaa gacctgatcg agcgagaggc gcaccgatcg tccggtggag
      901 agggtgagga gcacgacctc ctgggttgcc tctttatgca attgctccag tgcgtcggtc
      961 tttgaaaggt attcacgttc aagcgcggcg atctgttcga cgagacgccg gtctgcttca
     1021 atgctggcgg ggttatgtgc acctttcaac accagagtct ggcgcgcaag cctggccttt
     1081 tcgatacgat agttaatcga cccgatgtcc tcccgctcga gatgttcgat ccgctggcgc
     1141 aactggttgg cgcggtccac gagcgtttcg atggtgctcc aggcgctgcc atcagcgttg
     1201 gttgccgcag tgctttctgc tgcgaggctg atacggccgt aggcagggcc ccattcacga
     1261 cgctccacga acaccagatc gtcgggcgca gatatcgagg cgatctcgga ttcattgatc
     1321 caccggtatt cggaaccgaa ttgatcccga ttgccgacgc gataacgaaa gcgccgttgc
     1381 cccagtagcg catcagtcgt catcgaatgg ggaatcgttt cctcgccgac gaggtgtccg
     1441 atcaccgtct cctgcgtctt cagcgtcacc tgttgcaagg tgccgggcca aaactgcccc
     1501 aggccgttga acataatgag gaccagcatc ccggccacca tcagcaagct gatggccacg
     1561 ccggatgccg tcatccagac gaagagttct ccgcctcgcc aaaactcctt catgcgcaga
     1621 gatggcttac ccatggctat atttgtcccg gagtcgctgg cgaaccagtt cggcgagcga
     1681 attaatgaca aacgtgacaa tgaacaggag tagcgcggcc aggaacaaga cccggtacag
     1741 acttccgccg tgcggcgcct cggggatttc cacggcgatg ttggccgaca atgtccggaa
     1801 cccgttggcc cagttccagt ccaggatcgg agtatttcct gtggccatga ggacgatcat
     1861 ggtttctcca atagcgcggc cgaaaccgat catcacgccg gaaaagattc ccgggctggc
     1921 cgataacaac accaggtgca acacggtttg ccaacgcgtg gcacccaacg ccagagaccc
     1981 ggcaatttga gatttcggca cgttggagag ggcctcttcg gcgatgctgt agatcaccgg
     2041 cacgatggca aatcccatca cgatgccgac gaccaaggcg ttacgctgat catattggat
     2101 accgagccga ctagaaagcc aggttttgat gttgccgtca aaccaccaga cttcaaaaaa
     2161 tgaatgggcc cacagacacc cgccgatccc gagagccaga atgggcatta acagcagggc
     2221 ttcattccca ggggagaaat gccgtttgat agacatgggg cagagatgcc aagagaagga
     2281 cgccacgatg accagcacgg gcaaaaccaa caccatgccg gcaagcgcgg gaagcatccg
     2341 ttcgagcagg ggagctaacc agagcccggc cagaaacccc aggaccacgg tcggcaaggc
     2401 cgccatgact tcaataatcg gtttaacttt cgcgcgaagg tcgtgatgca tgaactgtga
     2461 ggtacagatg gccgcgaaaa tggcgatagg gacggcaaga aacagcgcgt atatcgttcc
     2521 cttgagagtc ccgaaggcca agggcagcag ccccagtttc ggctctacct cgtccgatcc
     2581 ggaggacgat tgccagacat aggctggttg accgtaccct tcgtaccaaa ccttgccgaa
     2641 gagagtgtcc agcgtcactt ccggataggg gttttgtacc tgatagagcg acagctgtcc
     2701 ggctccatcc aaggcaatca ccccatcgcc tttgggagca aaggccacgg cacgaatcgg
     2761 aaactcgccg ttggccactc gcaacagcgt ttgcgaagag gtcgcatggt gaaggaacac
     2821 attccctttt gtatcgcccg tgacaaagcc tttgacccgt tgagacgggg caaacgcggt
     2881 caccggagcc gcatgggaac ggaaggtatg catctgagtg agcgtccatc ccgtgggcac
     2941 atcggatcgc cgcaccaggc cccacgtcga cacggccccg tcagccgcca tcaccaccag
     3001 actgcgatct ccactgagaa atcccagcat actgatcgcc gtagtgggtc cggcgaccgc
     3061 atacgacgcc ctgacatccg gcggctgcgt ctctgacaca cccacatgga tgatgtgccc
     3121 atcggttgtg cccacataca gatgatcaag tgatgcttcc agcgccagag acgcgacgcc
     3181 acccgacggt tgcggcagtt ccgtgaccat tacacctgct cctgttggat cgtccggagc
     3241 aattctggcg aggaagagac gtcccgtctt ggtagcgagc gcaaggagac tgccctgatc
     3301 attggagcga taagccaggc gaacaatggg gctctccgtc accgggatgg gcgcgccggc
     3361 gcgaatgtga ggccgtttgc gtcgttcgcc ctgatcggaa aactctgtcg tcgtgccgat
     3421 cttcaacagc aagatttcgc cggctgcggt ccctacggcc agacgcgcag tggggccgcc
     3481 gccggaggcc atggccgtca cttgcctcga agtcagctga gccggcattt ccagtgcgat
     3541 gggctggccg gaggccagat caaaaaattg aatcgcaccg gacgtgaaga cctgcgcgat
     3601 ctctcgatgc tcatccaccg ccacctgcgc ggggccttcg tctgccagca aggccggcac
     3661 gctcaagcgt tggcttaact ttgcactcgg cgcggtgaaa agcggcgtga cctcgcgaaa
     3721 gagaaaaaag aagatgccga gaatgctgac gatggtcgcc agcccgccca ctgtaatgat
     3781 gccacgggcg agccggtcga tcactgaccg caagtggacc ggcccggaga gatagctcaa
     3841 aggattcgtg atcattaccg aatcttgccg agttgctgtt cagccaattt cgcatcgaca
     3901 gggaagtagc cgtctcgtaa cacgtcggac tggccttcct tgctgtatac gtaggccaga
     3961 aactccttga cgatagggga gagcggtttt cccggcgcct ggttcacata aatgtagagg
     4021 taccgccaca acggataggt tccgttcttc gtgttcgcct gcgtcggctc aatgtacggt
     4081 tgccccgcct tcgctgcgag cggcaccatt ttcaccccgg acgtgctata cccgatgccg
     4141 ctatagccga tgccataccg gtcctcgctg ataccctgca cgacagaggc agatcccggc
     4201 tgctccttca cttgatcttt gaagtctccg tttttgagcg catggtcctt gaagaatcca
     4261 taggtcccgg acgccgagtt gcgaccgtag aggctgaccg gcgcattcgc ccaatcgccg
     4321 ttctggccaa gctgtcccca gcgcgtcagg tccgtggtgt gcccctggcg tcgcgacttg
     4381 gaaaacaacg catccacttc ggcaatcgtg agtcctgcca ccggattgtc cttgttcacg
     4441 aagagcgcca gcgcatctac cgccaccgga taggcagtgg gagggtaacc gaacttcgat
     4501 tcaaaggcat cgatctcgct cgatttcatt gttcgcgaca tcggcccgag ctgagccgtg
     4561 ttttcgatca gggcgggcgg ggccgtgctc gatcctttgc cttctacttg aattttcacg
     4621 ttgggatatt gtttacggaa tccctccgcc cagagggtga tgagattgtt gagggtatcc
     4681 gatccgatgc tgttgatcgt tccagacacg ccgctgacct tatgataggg tttgataccg
     4741 gcatcaacag tgagcgtttc gaccgcggga gtatcacccg gcgctgcgct gaccaggcac
     4801 atcgtcataa atgggcctaa cgcggtcaac gtgagactca agcaaatctg gcgtgagacg
     4861 gtcatagaag acattcctcc ggttaaaaga gccgtaaact attgcaccgt cgagcatacg
     4921 gccgtttcat ttccagacca tcagcccaat gtaaacaccg tgttaactct cggtgagcct
     4981 cgctttctga tgaaagaatc gtgatcggct tctcgagtta accacaccga catctcgccg
     5041 tgaggaatct gaaaccgtcg ctccgtaccc tcttatgtca tgagaccctc gaccgtacat
     5101 atcgttgaag acgaaccgct ccacgccgga ctgctcgatc gtgcactacg ccttgcgggc
     5161 tttgacacct tgctgtccag cgacggcgcc agcgggtggc aggagattca acgccgccta
     5221 ccgacactca ttctgctcga cctcatgttg ccggcactca gtggtcaaga gatctgccgg
     5281 atggtgcgcc aacattccgc aacccggcac atcccgatca tcatgctgac agccgttgga
     5341 ggggaacccg aacggatcgc cggcctcgat atgggcgcag atgattatgt cgtgaagcca
     5401 ttcagtccga gagaggtcgt ctcgcgcgtg cgcactgttc ttcgtcgagg tgcacactca
     5461 gcggacgggg tcgcccccat tgtgaatgcg gctgtcagga tgcaggggcc gtattttgcc
     5521 gttctgttgg gggaacgaga actgctcctg tcccgaaagg agacggcgct gctccagacc
     5581 ttactcggtc gggaaaatgc cttggtggct gcagaagagt tgttaacgct tccggaaggg
     5641 gagaggccgg ggctgctact ccaagaactg gattacgacg tccgtggtct gcgtcgaaaa
     5701 ctggagaaca gcggggccgg atccgtcgac atgttgccgg gatttcgcta tcgattcagg
     5761 tcacggcttt gacgcccatc atacgtaagc aatccgcaga tcacgccttc acccaaagca
     5821 ggacaatgca ggttgacggg aagagatccg ttctatttcg taatcaaacc ctcttcgcaa
     5881 gagaatgatc ttcccatgcc ggctcaactc atcaatcgtc ttgaagacct gattccagct
     5941 cagttcggga aggagtgtga cgacctgctc aagcgtaagc gatttcctgc catgcaataa
     6001 ctccagaacc ctgcactcac tgctcagcac tgcagactga tgaaccatgg tggatcctcc
     6061 tggttggaat gtccccaagc cttctactcg ctgtccctgg caaccggctg actgctggat
     6121 cacatgcagc tgactgtgca acatgcgcct ctctttcaac tcaaccctgc atgaacatgc
     6181 gagcacttgt cacctgtacg cgaaggaatt agattccctc ccggcaggat cctgcgcatt
     6241 ttccgttgcc atcaccctga gcaggtgagc agcgcactgc gacgacacgt accgccccta
     6301 acgccagaac gatggcaccc aacgacagga gcttcgtttg gtcggcttct ttgaaccaca
     6361 gggaaatgat ctccgtcagc atgacgacca aaatcgtgtc gacaataaat gtcactttga
     6421 cgcgtccctc cgaaaaatag gtcagcgtcg tcttaaagac ttcgaccacc gccagaagga
     6481 tgagcgtatc cacaataatc tgccgaagcc caacctcaac cggagcctca agtaacaatt
     6541 tgatatccag aaatgtcttg atgaccccgc ccgtcagggc gatgaggatc gtcagaatca
     6601 gaagactcag cacggcttta atacccttca tccagaggtc ggtgagatcc gtttcgatgg
     6661 cgcgccctgc gagatctgcc acacggggaa cccgaacggg gaaactggta aacgtcatct
     6721 gtgaaacctc ctggtgaatg acacgatcat ggttgagcaa cgtgctcgag aggtccgtcc
     6781 ccggatcccc atggaccata accttcatcg ccactgtacc gctgaattgt tccgtgatat
     6841 tcacaacggc attacagcag cgttacgttt tctaggggcg agccgcctca gcggaccatg
     6901 cggacatctc actgcagtat ttacacagcc gtaacaaacg ggatacaggc aggcaactcg
     6961 atgccgctag catcaggcta tgcagcacgc gacggaaccg ctacgtcatc acggcattcc
     7021 ggtgcttctt gtcaccaaag acgaagaatt tgccatgtct gtccaccagc tgttacggcc
     7081 gaacgggtat catgtgtctc tcgcaccaac cgctgaggcc gccttgtctc ttgcttccgg
     7141 tgcctcgttc aaactggcgc tcatcgaccg gcgtcacaaa gtcgtcggga cgttgcggca
     7201 gaacctcgca ttccggcaca tcccgatgat cgcgcttcaa cccctgggag aaccgtgtac
     7261 ggaagaggag accgtggagg accttgccgc cggattcgat atcgtcatcg actcacatcg
     7321 gcatcgagaa tggctggcga tcatcaaggc cctgcttcga cgacaggaaa tacaagctgc
     7381 gcccctcagg gaatttcgag tcaatacgct atgcatgaat ctcgatcgac acgaggtgag
     7441 ggtcgaaggc aaatccatcc agctcacgcc gaaggagttc cagattctgc gcgtgctctt
     7501 gcagcatccg gggcgcgtgc tgacgcgcca ggaactactc aaccttgtct ggggagaaga
     7561 ctacgcgctc gaagaacatg ccttggacgt gcatatccac gcactgcgcc acaaattgga
     7621 aactaagccg tcggctccgc aatggattct cacggtcaga ggcgtggggt acaaactgtc
     7681 cacggattga gccgttgcca taagtcggca ccgcaacaga catcaatgac gcacgacacg
     7741 gttttacaca tcggtaacag agattgaacg tgggtgtgat gcctcgcaat tacaatccca
     7801 ccgctttcac gacattaaca gacactacaa ggaggatgtt cgtaatggaa atgcgtccgt
     7861 tggttgcatc catcagcggc cttgtcggga gcgcgatgct cgggctctgc gtaacagcgt
     7921 cctgggccga tgattcctca cgccagggcc agtcgtcaca caccgtggta gcccaggcaa
     7981 tgggcccggc tccgaacgga accagcagtc cgccgcctgc gccggatatg cctctgggcg
     8041 gcacgtcaat cgaagacctg cttatgcaga agggcacgat caccaaagaa gagtggattc
     8101 agattcgggc cgaacaggaa tacaaagctg gagagcggga caaacgggtc gatagcttgg
     8161 acgagtggaa gtccaaggct gagttgctgc cgatccttag ggataaggtg aacttcggac
     8221 tcaacgcact ccaatggttg tacacgcacc aggatgcgca tgtgcccgaa ggacggagtc
     8281 aggacaacct atccatccgc cggtcggaaa ttctcttctg gggccgtatc agcgacaccc
     8341 tgccgcgatg gcacaccttg atggagtttc aaagcatca
//
LOCUS       DAOQEW010000167         8288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2956_length_8288_cov_10.358071, whole genome shotgun sequence.
ACCESSION   DAOQEW010000167 DAOQEW010000000
VERSION     DAOQEW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2956_length_8288_cov_10.358071"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1435)
                     /locus_tag="PLS35_15240"
     CDS             complement(182..1435)
                     /locus_tag="PLS35_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNI20077.1"
                     /translation="MYRKIVLDNKLRVVAELLPTLKSVTIGIWVNVGSRDEQPGEEGL
                     SHFLEHMFFKGTRSRSATQISREIDALGGEMNAFTTRETTTFYVKVLDQQLEAALELL
                     SDLFYRSRFESKEVEKEKQVVLEEIRMVQDDPEDLVQELHMKHTLGSHPLGRPILGQA
                     PRIQALGRQDLVSYVGSHYDPERTVVAVAGNFTWKRLEQLLARYFSAPHKGVAVKQSR
                     RPPEVRGGVLVRRKALEQVHLCLGLQGLGAGHKDRYAAHALNGVLGGSVSSRLFQEVR
                     EKRGLVYSIYSFLSTYSDGGMTTVYAGTRPKEVERVVEVVCRELKKLRSKGIDAKDLA
                     RVKNQMKGSLMLSLESSHSRMSKLAKDELTQGSHVSLEQMIGEIDRVTTDQVYRVAQT
                     LLDQRCLAITALGPIPVNSLQSFAS"
     gene            complement(1442..3559)
                     /gene="pnp"
                     /locus_tag="PLS35_15245"
     CDS             complement(1442..3559)
                     /gene="pnp"
                     /locus_tag="PLS35_15245"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNI20078.1"
                     /translation="MKQVVDIELAGRRLTLETGRIAKQADGAIWATYGDTVVLATAVA
                     SQNAKPGVDFLPLTVDYQEKTYAAGKIPGGYFKREGRPSEREVLTSRLIDRPLRPLFP
                     EGYYFETQVITSVLSADKTGVSDVIAITAASAALAISSIPFNGPIAGVKIGRVNGQFV
                     VNPDLETLETSDLHLVVAGTADAVMMVEAGANELPEATMLEAIELAHSEIKKIVAKIE
                     ELRTLAGKPKRVVTQEPIDAALAEQVRKLVAGPIREAILIPNKSARQERLDQVLAETL
                     VALKSDEPNRDRHVKIIFHGLEYTEVRNMILEKRVRADGRGPSDIRPITCEVSVLPRA
                     HGSAVFTRGETQSLAVVTLGTTDDEQRIDALEGEYMRTFMLHYNFPPFSVGEARPLRS
                     PGRREVGHGALAERALKSIIPGKDKFPYTVRIVSEILESNGSSSMATVCGGTLALLDA
                     GVPIKEPVAGIAMGLIKEGDQVLVLSDILGLEDHLGDMDFKVTGTKNGVTALQMDIKI
                     GGIDAALMREALAQAKAGRLHILGCMAQALTAPRAKLSAFAPRIFPMKIKQDKIRDVI
                     GPGGKMIRSIIAETGVKINVEDTGDVTIASSDEASAQKAIDMIKRLTEEVEVGKIYLG
                     TVRKIMDFGAFVEVLPGTDGLVHISQLAHHRVKAVTDEVKEGDQIQVKVLEIDKQGKI
                     RLSRKEAMPAPAGSPATEPTPAG"
     gene            complement(3719..3988)
                     /gene="rpsO"
                     /locus_tag="PLS35_15250"
     CDS             complement(3719..3988)
                     /gene="rpsO"
                     /locus_tag="PLS35_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNI20079.1"
                     /translation="MALVKEVKTELMKNFQQHDKDTGSPEVQIAILTNRITYLTEHFK
                     LHKKDHHSRRGLLTLVGRRRRLLDYLRHVEEGRYRAVIERLGIRK"
     gene            complement(3995..5023)
                     /gene="truB"
                     /locus_tag="PLS35_15255"
     CDS             complement(3995..5023)
                     /gene="truB"
                     /locus_tag="PLS35_15255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNI20080.1"
                     /translation="MMIASTESQTATMLQDGVLNVRKEAGWTSHDVVARVRGKLRGMK
                     LGHAGTLDPAATGVLPLLVGRGTRIAEYLLEWDKTYLAGLRLGETTDTQDATGTVLQR
                     SPITALTEARIRETIAGFEGRIQQVPPMYSAVKVGGVPLYKAARAGKDVARQPREVTV
                     FRLEVVSIQLPDVMLRVVCSKGTYIRTLCADIGEKLGVGGHMATLIRERVGPLMVEQS
                     LTVNEVETRLTQGTLAGSMLTLDEALVGLPVCIIGRETARQVVHGMAVPTADVLSWQG
                     LPTILPEASGGPIRIKDTAGRLLAIGTMPAGLGDQARMPGELSIAMSKVLVEDESQGS
                     SIANSIEE"
     gene            complement(5027..5425)
                     /gene="rbfA"
                     /locus_tag="PLS35_15260"
     CDS             complement(5027..5425)
                     /gene="rbfA"
                     /locus_tag="PLS35_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNI20081.1"
                     /translation="MTKSTYSRAERVADQIRMEVADILMRKIKDPRVRSVTVTDVELT
                     KDLRIARVFVTTMEQNKDERLVFDGLAKASGFVRAELGRRLALRYLPELIFMKDVSGP
                     RADRVLALLDGLHLDETKEDRLPETPRTDV"
     gene            complement(5435..5716)
                     /locus_tag="PLS35_15265"
     CDS             complement(5435..5716)
                     /locus_tag="PLS35_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNI20082.1"
                     /translation="MIVGLCTVELFIPDGHSLKDKRQVLQSLKSRLRDKFNVSVAEVG
                     EQELWQKAILGLACVANESAHVNQVLDQAVNLIQGVPTIQLLRSRIELL"
     gene            complement(5713..>8288)
                     /gene="infB"
                     /locus_tag="PLS35_15270"
     CDS             complement(5713..>8288)
                     /gene="infB"
                     /locus_tag="PLS35_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNI20083.1"
                     /translation="ELAKQLGMENRELIPELKRLGIPVASHSSALDDDSVRMALEKLG
                     SKARAGETSSSGHEAKKMTRLPKESAGSHAAAHEEAPKPDKKRILIKKKREEGAEDVA
                     ASLAAAEAVFAPAAPAAADAGVVDQAPLPGSEVSEAGPIEPVQAQEPEPPPPVVATSE
                     DVTTKPSQAPVVTTPPVDPLAAIKKKSLVPDASESEAAAREKLKKAKKAPRTREEDEA
                     KFKNDATRWGDLRAIPVQRREDRSKHIHHASPTEITKPRKKSVKLTAGVSVKEFAELI
                     GQRPADVVRKLMDMGQMVTFNQSINLEAASLIAEEYGARVEVSTEKVGEALLEEAAQS
                     SGEEHAVSRPPVVTIMGHVDHGKTSLLDAIRSTKVAEGEAGGITQHIGAYIVGVHDKQ
                     VTFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTVEAIHHAKAAGVPLIVAI
                     NKVDKPGANVDRVKNALTEHGLVAESWGGDTIMVEVSAKQRTGLDQLLEMILLQAEVL
                     ELKADPTRMAKGLVIEAKLDRGRGPIATVLVQSGTLHVGDAFVVGNFSGRVRALVTDT
                     GKKTTEAGPSVPVEVIGLPGVPSAGDAFTIVKDERVAREIAQERAMKQRAADLAGPAK
                     VSLDDLFAKIQEGNVKELPIVIKADVQGSSEALAAAVEKMPAGAVKLRVMHTGVGGIT
                     ETDILLAAASKAIVIGFNIRPEPKAAALAEREGVDVRLYSIIYDALNDIRAAMEGLLE
                     PTLKERILGRAEVRQMFTIPKAGLVAGCYVVDGVISRASVGVRVIRDSVVVYEGKLGS
                     LRRFKDDVREVQQGYECGITVENFNDLKAGDIIEAFAIDKVAAKLESAHRGTSPQPHR
                     A"
BASE COUNT         1673 a         2636 c         2137 g         1842 t
ORIGIN      
        1 gcggccatgg tacttaaatc caccaaacgt gtcaagaaaa tgtttttcga aaattgtacg
       61 gcagttgcca cgatcaaaat gccgcccgat caatcagtta gcggtcagaa gcacgtcgat
      121 ggttgcttct ctctcacgct cagttgctgc gccgaccgag gagatgcagg cgcgtgatct
      181 attatgacgc aaacgactga aggctattca ccggtatggg tccaagcgct gtgatcgcga
      241 gacaccgctg atccaacaag gtctgggcca cacgatagac ctgatccgtc gtcacacggt
      301 caatctcccc gatcatttgt tcgagcgaaa catgactccc ttgcgtcaat tcgtctttcg
      361 cgagtttact catccggcta tgcgagcttt ccaggctgag catgagactg cccttcattt
      421 gattcttcac gcgggcgaga tcttttgcat caatcccctt gctgcgcagc ttcttgagtt
      481 cccggcacac gacctcaact acacgctcca cctctttcgg gcgcgtgccg gcatacaccg
      541 tcgtcatccc gccgtccgag tatgtggaca ggaacgaata aattgaatac acgagaccac
      601 gcttctctct cacctcctga aacaggcggg aactcacgct tccgcccagc actccattga
      661 gcgcatgagc cgcataccga tctttatgtc cagcgcccaa tccctgcagg cctaaacaga
      721 gatggacctg ctccaacgcc ttacgcctca ccagcactcc tccacggact tcgggtggcc
      781 gccgactctg cttgaccgcg acgcctttgt gaggggcgga gaagtagcgg gccaggagtt
      841 gttcgaggcg cttccaggtg aagttccccg caacggcaac aaccgtgcgc tcaggatcgt
      901 agtgagaacc gacatacgag accaggtcct gacgacccaa cgcctggatt ctcggagctt
      961 gccccaggat aggccggccc aacgggtgac tgccgagcgt atgcttcatg tggagttcct
     1021 gaacgagatc ctccggatca tcctgaacca tccggatctc ttccagcacc acttgttttt
     1081 ctttttcgac ttccttggac tcgaaccgcg aacggtagaa cagatcggag agcaactcca
     1141 acgccgcttc caattgttga tcgagcactt tcacataaaa cgtcgtcgtc tcgcgagtcg
     1201 tgaaggcatt catctccccg cccagcgcat caatttcacg ggaaatctgc gtcgccgatc
     1261 ggcttcgcgt ccccttgaaa aacatatgct cgagaaagtg agacagtccc tcctcgcccg
     1321 gctgctcgtc gcgggagccg acattgaccc aaatgccgat cgtgacagac ttcaacgtcg
     1381 gcagtaattc ggccacgacc cgcaacttgt tgtcgagaac gatcttgcga tacacgatcg
     1441 attatcctgc gggagtcggc tcagttgcgg gagagcccgc aggtgccggc atcgcttctt
     1501 tccggctcaa acggattttc ccctgcttat cgatttccaa cactttcacc tggatctggt
     1561 caccttcctt gacctcatcg gtgaccgcct tgacgcggtg gtgcgccaac tgagagatgt
     1621 ggaccaaacc gtcggtgccc ggaagcactt cgacgaaggc gccgaaatcc ataatctttc
     1681 gaacggtccc cagataaatc ttgccgacct cgacttcttc ggtcagacgc ttgatcatat
     1741 cgatggcctt ctgtgccgag gcttcgtctg aggacgcgat cgtcacatcg cccgtgtctt
     1801 cgacattgat cttcacaccg gtctccgcga tgatgctgcg gatcattttt ccacctgggc
     1861 cgatgacatc acggatctta tcctgcttga tcttcatcgg gaatatgcgc ggagcgaagg
     1921 cagacaactt ggccctggga gccgtcaacg cctgcgccat gcagcccaga atatgcagtc
     1981 gaccggcctt ggcctgcgcc agcgcttccc gcatcaacgc ggcgtcgatt cccccgatct
     2041 tgatgtccat ctgcagcgcg gtgactccgt ttttcgtccc cgtcaccttg aaatccatat
     2101 cgcccagatg atcttccaag ccgagaatgt ctgagaggac caagacctga tcaccttcct
     2161 tgatcaggcc catcgcgatg cccgccaccg gttccttgat cggcacaccc gcatccagca
     2221 aggccagcgt tccgccgcag accgtggcca tggacgagga gccgttggat tcaagaattt
     2281 ccgacacgat tcgaacggtg taggggaact tatctttccc ggggatgatc gacttcaatg
     2341 cgcgctctgc tagtgccccg tgcccgactt cgcggcgccc aggggatcgc aacggcctag
     2401 cctcgccgac actgaagggc gggaaattgt agtgcaacat gaaggtgcgc atgtactcgc
     2461 cttccaaggc gtcgatgcgc tgttcatcgt cagtggttcc caacgtcacc accgctaagc
     2521 tttgcgtttc cccgcgggta aataccgcag agccgtgcgc gcgtggcaac acactcactt
     2581 cgcacgtgat cggacgaatg tccgacggac cgcgcccatc ggcacgcacc cgtttctcga
     2641 gaatcatgtt gcgaacttcg gtgtactcca agccgtggaa aatgattttg acatgccggt
     2701 cgcggttggg ctcgtccgac ttgagcgcga caagcgtctc ggccaggacc tgatccaatc
     2761 gctcctgccg tgcgctctta ttgggaatca gtatggcttc acgaatcggg cccgccacca
     2821 gcttgcgcac ttgttcggcc aaggcagcgt cgatcggttc ttgcgtcact actcgcttcg
     2881 gcttaccggc cagggtgcga agctcttcga tcttcgcgac aattttttta atctcgctgt
     2941 gcgccaactc gatcgcctcc agcatggtcg cttcaggcaa ttcattggcg cccgcttcga
     3001 ccatcatcac agcgtcggca gtgcccgcca ccacgagatg tagatcgctc gtctcaaggg
     3061 tttccaagtc gggattcacg acaaactggc cgttcacccg accgattttc acacctgcga
     3121 tggggccatt gaacggaatg gaggaaatgg ccaacgcggc agacgcggcg gtgatcgcaa
     3181 tcacatcgga cacgccggtt ttatcagccg agagcaccga agtgatgact tgggtttcga
     3241 aatagtagcc ttcgggaaag agggggcgca gcggacgatc gatcaatcgg ctggtgagca
     3301 cttcccgctc ggagggtcgc ccttcccgct tgaaataccc gccgggaatc ttgccggccg
     3361 cgtaggtctt ttcctggtaa tccaccgtca gcggaagaaa gtccacacca ggcttcgcgt
     3421 tttgtgaggc gacagccgtg gcgagcacca ccgtgtcgcc gtaggtggcc caaatcgcac
     3481 cgtccgcctg tttggcaata cggccggtct ccagtgtcaa gcggcgacct gccagctcga
     3541 tatctactac ttgtttcatc tatggtctcc tcccgttaaa tcccacagat gcccacagag
     3601 ataagctcaa ttcgaatgcg gcccctagat ggcccatgcg tttgaacttg tttcagtgtt
     3661 cacctgcggg gacaatagtt cggccgccac acatgtggcg gccgattcga tcacacgact
     3721 acttacggat ccccagacgt tcgatcacgg cgcgatagcg gccttcttcg acgtgccgga
     3781 gataatccaa caagcggcga cggcgtccga ccaacgtcaa caatccacgc cgggaatggt
     3841 ggtccttctt gtgcaattta aaatgttccg tcaaatacgt aatccggttc gtcagaatgg
     3901 cgatttgcac ttccggagaa ccggtgtcct tgtcatgctg ctgaaaattc ttcatcaact
     3961 ctgtcttcac ttctttgacc agtgccatac gtctctactc ctctattgag ttcgcaatac
     4021 tactaccctg tgactcgtcc tcgactaata cctttgacat cgcaatcgac agctcgcccg
     4081 gcatcctggc ctggtcgccg agtccggcgg gcatggtgcc gatcgcgagc aatcgtccag
     4141 ccgtgtcctt gatacggatg ggtccacccg aggcttccgg gagaatggtc ggcaagccct
     4201 gccaactcaa cacatcggcg gtcggaactg ccatgccatg tacgacctgc cgtgcagtct
     4261 cccggccgat gatgcagact ggaagtccta ccaacgcctc atccaacgtg agcatcgatc
     4321 cggccaacgt cccctgagtc aggcgtgtct cgacttcatt gacggtcaag gactgctcta
     4381 ccatcagcgg cccgacgcgt tcgcgtatca aggtcgccat atgaccgcca accccgagtt
     4441 tttctccgat atccgcgcag agcgtcctga tatacgttcc tttggagcag accacacgga
     4501 gcatgacatc aggcagctga atgctgacga cttccaaacg aaagacggtg acttcacgag
     4561 gctggcgggc cacatccttc cctgctcgcg cagctttata gaggggaacc cctcccactt
     4621 tcacggctga atacatggga ggaacctgtt gaatgcggcc ttcaaaccca gcaatagttt
     4681 cccggatacg agcttcggtc aaagccgtaa taggcgagcg ctggagcacc gttccagtgg
     4741 catcttgcgt atcggtcgtt tcacccaatc gcagtccggc cagataggtt ttatcccatt
     4801 cgagcagata ttcagcgatg cgcgtgccac ggccgaccag cagcgggaga accccggttg
     4861 cagcgggatc gagggtgccc gcatggccca gtttcattcc gcgcaatttc cctcgaacac
     4921 gggcgaccac atcgtgtgac gtccatccgg cttctttgcg gacattcagc acgccatcct
     4981 gaagcatggt agccgtctgg gattccgtcg acgcgatcat catcggctag acatcggtgc
     5041 gaggggtttc tggtagcctg tcttctttgg tttcatccag gtgcaatccg tccaacagcg
     5101 cgagaacccg atcagccctg gggccgctga cgtctttcat aaaaatcaat tccggcagat
     5161 accgtaacgc cagtcgccgc cctagttccg cccgaacaaa accactcgct ttcgctagtc
     5221 cgtcaaatac cagccgctcg tctttgttct gctccatggt ggtgacaaag acacgcgcaa
     5281 tgcgcaaatc tttggtcaac tccacatcgg tcaccgtcac ggaacgcacg cgcggatctt
     5341 tgatcttgcg cattaatatg tcggccactt ccatgcggat ctgatcggcc acccgttcgg
     5401 cccgactata cgtcgatttc gtcatgacgt ggccttacaa caattcgatc cgagagcgga
     5461 gcagctgaat cgtgggcacc ccctgaatca aattcacagc ctggtccagc acctggttga
     5521 cgtgggcaga ttcattcgcc acacaggcca atcccaaaat cgccttctgc cacaattcct
     5581 gctctcctac ttcagccact gacacgttga acttatcccg caagcgactt ttcaaactct
     5641 gcaaaacctg ccgcttgtct ttcaatgaat gtccatcggg gatgaacaac tcgaccgtgc
     5701 aaagcccgac gatcatgccc gatgaggttg cggggaagtg ccgcggtgtg ccgattcgag
     5761 ctttgccgcg accttgtcga tggcaaaggc ctcgataatg tctcctgctt tgaggtcgtt
     5821 gaagttttca accgtgatac cgcactcata gccttgctgg acttcccgca catcgtcctt
     5881 gaaacgcctc agcgagccca gcttcccctc gtagaccacc acactatccc gaatcacacg
     5941 cacgccgaca ctggcgcgcg agatgacgcc atccaccacg tagcaaccag ccaccaggcc
     6001 ggccttggga atcgtaaaca tctgccgcac ttcggcacgt ccgagaatcc gctccttgag
     6061 cgtgggctcg agcaaccctt ccatagcggc acgaatatca ttcaatgcgt cataaataat
     6121 gctgtagaga cggacatcta caccctctcg ttccgccaac gccgcggcct ttggctcagg
     6181 acggatgttg aatccgatga caatcgcctt cgacgcggcc gccagcagga tatcggtttc
     6241 ggtaatccct ccgactccgg tgtgcatgac gcgcaatttg acggcgccgg ccggcatttt
     6301 ctcgacggca gcagccaagg cttcggagga accctgcaca tcagccttga tcacgatcgg
     6361 cagctctttg acattgccct cttggatttt ggcgaacaag tcatccagac tgactttcgc
     6421 cgggcctgcc agatcagcag cccgctgctt catcgctcgt tcttgcgcaa tctcgcgggc
     6481 gactcgctca tccttcacga tggtaaaggc gtcaccggca gagggcacac ccggcaatcc
     6541 gatcacttcg accggcaccg atggtcccgc ttcggtggtt ttctttcccg tatccgtgac
     6601 taacgcgcga acccggccgc tgaaattacc gacgacaaac gcgtcgccta cgtgcaaagt
     6661 cccgctttgc acaaggaccg tcgcgatggg gccgcgcccg cgatccagct tggcttcgat
     6721 caccaagccc ttcgccatgc gcgtggggtc cgctttcagc tccaagactt ccgcctgcaa
     6781 gaggatcatt tccagaagtt gatccagacc ggtccgctgt ttcgcggaaa cttccaccat
     6841 aatcgtgtca ccaccccacg attcggccac caacccatgc tcggtcaggg catttttcac
     6901 acgatcgaca ttggcacccg gcttatcgac tttattgatg gcgacgatca gcggtacgcc
     6961 ggcagccttg gcatgatgaa tggcctcgac tgtttggggc atgacgccat catctgcagc
     7021 aacgaccaaa atgacgatgt ccgtcgcctt cgctccccgt gcccgcatcg cggtgaacgc
     7081 ttcgtggcca ggagtatcga ggaacgtgac ctgcttgtca tgaaccccga cgatataggc
     7141 gccgatatgc tgcgtaatcc ctccggcttc gccttcggcg accttcgttg aacgaatcgc
     7201 gtccagcaga gacgtttttc catgatcgac atggcccatg atcgtgacga cgggaggccg
     7261 cgacaccgcg tgctcttcac cggatgactg cgccgcctct tccagcaagg cttcgccgac
     7321 tttctccgtc gacacttcca ccctggcgcc atactcttcg gcaatcaacg acgccgcctc
     7381 gagattgatc gactggttga aggtcaccat ctgccccata tccatcagct tccggaccac
     7441 gtccgccggc cgctgaccga tgagttccgc aaactctttg acgctgactc ctgcggtcaa
     7501 tttcacgctt ttctttctcg gcttggtgat ttccgttggc gaggcatggt gaatatgttt
     7561 ggaccggtcc tcacgccgct gcaccgggat cgcccgcaaa tctccccacc gagtggcgtc
     7621 gttcttaaac ttggcttcat cttcttcccg ggtccggggc gccttttttg cctttttcaa
     7681 tttctcgcgc gcagcggctt cgctttctga tgcatcggga acgagacttt tcttcttgat
     7741 tgcggcaagg ggatcgacag gaggagtcgt gaccacagga gcctgagacg gcttggtcgt
     7801 cacatcttcg gacgtcgcga caacgggcgg cggaggctcg ggctcctggg cctgaaccgg
     7861 ttcaatcgga ccagcctcac tcacttcaga acctggcagg ggggcctgat ccacaactcc
     7921 ggcgtctgcc gctgcgggag cagccggggc aaacaccgct tccgctgcgg caagcgacgc
     7981 cgcgacatct tctgcgccct cttcacgctt cttcttgatg agaatgcgct tcttgtcggg
     8041 tttcggcgcc tcttcatgcg cggctgcgtg cgatccagcg gactctttcg gcaaacgtgt
     8101 catcttcttc gcctcatgcc cggaagaaga cgtctcaccg gcacgcgcct tcgagccaag
     8161 cttttcaagc gccatgcgga ccgaatcgtc atccaacgcg ctgctgtggg atgccaccgg
     8221 aatcccgagc cgcttcagtt caggaatcag ctcccgattt tccatcccca gctgctttgc
     8281 taattcgt
//
LOCUS       DAOQEW010000168         8257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2973_length_8257_cov_8.022799, whole genome shotgun sequence.
ACCESSION   DAOQEW010000168 DAOQEW010000000
VERSION     DAOQEW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2973_length_8257_cov_8.022799"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..3209)
                     /locus_tag="PLS35_15275"
     CDS             complement(180..3209)
                     /locus_tag="PLS35_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20084.1"
                     /translation="MKKRKSLFGHFASWMARNVPHLWSGQPDPGYTPEFLETQLSNLL
                     ATESELAAHRILANWWDILLGEGHRLKRWNVRPGSQRVKLPPERPSFVRRDFQLLTRL
                     RPIEAAFLDRLGSSLDSRQRYHAFMLSAVLNGGILSTERLSALLGLSADAVQMIDGVL
                     WATLNLPVSGSPNGRPLRWYPDALTATLLTKVLENGPLLHPEGNVRAGRAVLWSRLEA
                     TLNTLELPPWRGELNELLRAVRVSTALSAPGFVAAYLAEDLESHSLPDPVFQRVLGAR
                     LPAADSPSAEPKHAPPLAPAAIELDVDFDPDLPRNSQMSITRQVCKTLDNKRGAIDRL
                     KKILEEHHGRLWPITHALIGWTMWRLDPGCPLGKIKASSAQTYFRTLAVHLIYEAEDL
                     DLLDLDVDDFETLYELAARRVGTESRRAYFWGRLRDFHDYLFLGGAPDIDLRELDGWV
                     AKGSVRVSANLVTEREFQRFKKALEMNRNHPTAEMVFLAGMLGYRAGLRRREAQMLRI
                     HDVHPGPDPFLLIRPSKLASLKSNAAKRRIPLRALLPVDELTILMDFHDRRKAQADGK
                     DGLLFASPDAPWTPTPYSTLIDPITALFAAITGQVGVPFRFHHLRHSFANWVLVAMLA
                     TDEPELLTRKLPLLDAHLLSNASQVLLRTRFYPTLEGSAHYPERRHLYQVAALMGHLS
                     PSTTCQSYLHLLDWLSGRYLDLALDHRLRHLGGPDLGRICGLSSSMPYKREYREFAHS
                     AVAFLRHHVAMRAKAQAPIIEHAPAARTLPTLSDLIKPTLPTPSLLITLLRRHFSGTS
                     IQKLESIFAIPASAITAATEAYLRMYAKQSVQHPKGTIPVPRPPRSMEQQCEYFRIVE
                     RAYASARNAENRLAMSALAEALIQRTGPKTGKIYFGTEGRDFKMILKGLTLLGVDAAE
                     MTLIVRRPAAIREDPDFVTNAVDHAKSQGVAIEGQPLEWEKREVKGVLLRLDVRATQP
                     VVALKGQHLEGRIRGLNHAAAWIRFIDHLPSVRTICG"
     gene            complement(3275..5791)
                     /locus_tag="PLS35_15280"
     CDS             complement(3275..5791)
                     /locus_tag="PLS35_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20085.1"
                     /translation="MNSREEILHLLAPLTSILRMPDDLIGILAVARRLVPVLRSLVRV
                     TDEKQQPLISACLAGLEQVIDGASSVAQFFPEALGGGRRILKGYYASHPHYCVFWHVC
                     ADPELEQQYGTLLVSMFPVQMRLERDRTLINTVADPAYMVGLSLRQLTDRPDESLRPF
                     LLELPLEPMHPQALLEHFENHMSRLGLRPLVTGTLRNLGYLHRALQWFETGVWEVQHH
                     LQTGRADTHKAGRHGAIDGTQPSRSISRTLEGDDGLPDIGVTQFVEDDGSTHRHDRDL
                     DFSPPGDGPKRGTLIAVAPRSASAANRGDRRALARNVARYTAQAITLANQQLPVTHAT
                     LSGYEMELLVDLIDNLTTDDWGEISASDRPLVAAWAACRLFLSRAPEAIKTLQIRKVR
                     TAPHNRARVPPTILWTPTTGRIWLPVASPKHVTPRIEGEPAIRTLAAAKGFELEVPPA
                     LTTALSRVEHAQEPLFQRSYEPEFAQLLAALNQTRGTALHPDRVGRLLAKFMAQLGGN
                     DRVYEFYFRGIPPNQHNPCVYSAVPVSRLQTLYAGACARLYQLAGLPYPAATATAPTS
                     APEYGATHVGSLHVPTRASVRTTVLTVLNAVAKLASEAGVKYVERHNAYTAYVAFFLL
                     ATTGLRGVSSLLPAHFDIDRATGLCFVSDKDNESYEQSRIVWLHPMLLDQLRAYSQHV
                     TRLRQYLALANPEGIDHLDARDRLVSLSSHTAPNRNRDLELLRAATPSLFLLTKHGSQ
                     LTPTFPLELQHLLGSTWQLRIGALRHFFRTELLHLGVPGPVINATLGHGERGEGPWGR
                     FSSLPPLQWQRLAAAAITKICDASGFRVLQSPLLRSLSTS"
     gene            complement(5803..6312)
                     /locus_tag="PLS35_15285"
     CDS             complement(5803..6312)
                     /locus_tag="PLS35_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20086.1"
                     /translation="MREEALSLLPLLQPLVVREEPGGATSNARRYILLAGRRTLQLLL
                     EKYSRDHLTWALCVANDAPLPNKDELELLDTLLIKLICRPDPDDLALIATALKSDQVL
                     RSEAGRYVDVTSDAAIGRLVGMSRTAMHRHTTRNRSKIDETDKAAKSVTLDLLQEAEP
                     PPVEHGTSD"
     gene            complement(6508..7902)
                     /locus_tag="PLS35_15290"
     CDS             complement(6508..7902)
                     /locus_tag="PLS35_15290"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNI20087.1"
                     /translation="MSRIAPTEELKLIESIVADHPKGLGISAIEAEIERRQGMRPNRR
                     TLQRRLQKLIDARRLTKEGESIALVYKSTADAEPRTDEVKAPETDLYVPVSPEGAAIR
                     DRMRLPLMHRRPVGYRRDFLEAYQPGATFYLPESLRSQLHEMGRTPVGERPAGTYARE
                     ILGRLLVDLSWASSRLEGNTYTRLDTQNLIEYGQVAQGKDAIETQMILNHKAAIEMLI
                     ENADEIGFDPFTFKNLHSVLSQDLMRNPNACGRLRRRPVDISGTVFHPLAMPQVVEDC
                     FALLLGKTAAIPDPFEQAFFVMVQLPYLQPFEDVNKRVSRIGANISLIKHNLCPLSFI
                     DVPERAYVEGTLGVYELNQIELLRDVFVWAYERSCQRYLAISQTMVEPDPLKIKYREV
                     LIQAVQAVVKGLQPPNQAVIARLAAENAPESDRAAFAELLTAALMQLHEGSVARYRLR
                     RSEYLAWQRLRGDA"
BASE COUNT         1644 a         2273 c         2617 g         1723 t
ORIGIN      
        1 ggggcgattc gaggcaaaaa cggggcaact ccgccgagaa acacggtggg ttgccggaga
       61 aattggcccg aactcggttg ccgtcatcat tgcgcggcgg gattcactga aaacctcggg
      121 ttattcgagg cgcccggacg gcggcctgtg ggcctattgg acggcggcag tcatccagac
      181 tacccgcaaa tcgtacggac agagggcaaa tgatcaataa agcgtatcca cgctgcggca
      241 tgattcagcc ctcgaatacg cccttccaag tgctgcccct ttagagcaac aacgggctgg
      301 gtggctcgca catccaaccg taagagaacc cctttcacct ctcgcttctc ccattcgagt
      361 ggttgccctt cgattgcaac accctggctc tttgcgtgat cgacggcatt ggtgacgaaa
      421 tcagggtctt ctctgatggc tgccgggcgg cgtacgataa gcgtcatttc ggccgcgtcg
      481 actcccagga gggtaagacc tttgaggatc atcttgaagt cgcggccctc cgtcccgaag
      541 taaatcttcc ccgtcttggg acccgtacgc tgaatcaagg cttctgcaag tgcactcatg
      601 gcaagacgat tctccgcatt acgggctgag gcgtatgccc gctcgacaat tctgaaatac
      661 tcgcattgtt gttccatgga gcgaggtgga cgcggcacgg ggatggttcc ctttgggtgc
      721 tgcactgatt gcttggcgta catgcgaagg tatgcttcgg tagcagcagt gattgcactg
      781 gctggaatcg cgaaaatact ctccagcttc tggatcgatg tgcccgagaa gtgtcttcga
      841 agcagcgtta tcagtaagga tggtgttggc agggtcggct tgatcagatc actgagagtg
      901 ggaagtgttc gtgctgcggg tgcatgctca atgattggtg cctgagcttt ggcacgcatc
      961 gccacatggt gacgcagaaa ggcaactgcg ctatgggcga attcacgata ctcccgcttg
     1021 tagggcattg aactggataa cccacagatg cgcccaaggt ccggtccacc gaggtggcgc
     1081 aagcgatgat ccagtgcaag gtcgagatac ctgccactga gccaatcgag gagatgaagg
     1141 tagctttgac acgttgtgct cggactcaag tgccccatca gggcggcgac ttgatagaga
     1201 tggcgacgtt ctggatagtg cgcgctgcct tcaagcgtcg gataaaaacg cgtgcgcaac
     1261 agcacctgac ttgcgttact gagcaggtgc gcgtcaagaa gcggtagttt tcgtgtcagc
     1321 agttccggtt cgtccgtagc gagcattgcg accaggaccc agttggcaaa tgaatgtcgc
     1381 agatgatgga aacgaaaggg caccccaacc tgcccggtga tcgcggcgaa gagcgccgtt
     1441 atcggatcaa tcagcgttga atacggtgtc ggtgtccagg gggcgtcggg gctcgcgaac
     1501 agcaagccat ccttgccgtc ggcctgggcc ttccttcggt cgtggaaatc catcaagatc
     1561 gtaagctcat caaccggcag cagcgcgcga aggggaatcc ggcgcttggc tgcattcgac
     1621 ttgagagatg caagcttcga tgggcgaatc agtagaaagg ggtcaggccc cggatggacg
     1681 tcgtggatcc tgagcatctg ggcttcacgc cgtcgcaaac cggcgcggta acccagcatt
     1741 ccggcaagaa agaccatttc tgcggtagga tgattccggt tcatctccag ggcctttttg
     1801 aagcgctgga actccctttc cgtgacgaga ttcgctgata ccctgacgct ccccttggcg
     1861 acccacccgt cgagctcgcg cagatcaata tcgggcgcac ctcccagaaa caggtaatcg
     1921 tggaagtcgc gcaaccgacc ccagaagtac gcgcgacggg actcggtgcc gacccgtctg
     1981 gcggcgagct cgtacaaggt ctcgaagtca tcgacatcca gatcaagaag gtcgagatcc
     2041 tccgcttcgt agatcaaatg taccgccaag gttctgaagt aagtctgcgc gctggatgcc
     2101 ttgatctttc ccaacggaca tccggggtcc aatcgccaca tcgtccagcc gatcagcgcg
     2161 tgggtaatcg gccacaacct gccgtggtgc tcctcaagga tcttcttcag acgatcgatt
     2221 gccccgcgct tgttgtccaa ggtcttgcac acttgccggg tgatactcat ctggctgtta
     2281 cgtggcaagt cgggatcgaa atcgacatcc agttctatgg cggctggcgc aagagggggc
     2341 gcgtgtttcg gctcagccga aggactatca gcggctggaa gccttgcgcc caatactcgc
     2401 tgaaagacgg gatcaggcaa ggagtggctt tctaggtctt ccgcaagata ggccgcaacg
     2461 aagccggggg ccgaaagggc ggtggagacg cgtactgcgc gcaacagctc gttgagttcc
     2521 cctcgccatg gcgggagctc gagtgtattg agagtcgcct cgagacggga ccaaagtact
     2581 gcgcgacccg cgcggacatt gccctccgga tgcagcagcg gcccgttctc aagaaccttg
     2641 gtcagtaaag tcgctgtgag cgcgtcggga taccacctaa gcggccggcc gttcggtgat
     2701 ccggaaaccg gtaggttgag cgttgcccac aggacaccgt cgatcatttg caccgcatcg
     2761 gcgcttaggc caagaagtgc gctcagtcgc tcggtggaga gaatcccacc gttcagtacg
     2821 gccgaaagca tgaacgcgtg gtagcgttgt cttgaatcaa gagagctgcc gagccggtcg
     2881 agaaatgccg cttcgatcgg gcgaagccgc gtgaggagct gaaagtcccg ccggacgaag
     2941 ctcggtcgct ctgggggcag cttgactctt tgcgaccccg gacgaacatt ccagcgtttc
     3001 agacggtggc cttctccaag cagtatgtcc caccagttcg caagaatccg gtgggctgcc
     3061 agctcggatt cggtggcgag aaggttggat agctgggtct caagaaattc cggggtataa
     3121 ccgggatccg gttgcccgct ccaaaggtgc ggtacattcc tggccatcca gctggcaaaa
     3181 tgaccaaata gggatttgcg tttcttcaag tccctcggtg tggaggcaac tgcccaatcg
     3241 ggccatcccg tgctcggtgc ttcgacccat cgagtcatga agtgctgaga cttctcagaa
     3301 gtggactttg aaggacccga aagcctgagg cgtcacagat cttggtgatt gccgcggcgg
     3361 ccagacgttg ccactgcagg ggagggagcg aactgaatcg gccccaaggt ccctcgccgc
     3421 gctcgccatg gcctagcgta gcgttgatta ccggccccgg aacaccaagg tgcagcagtt
     3481 ctgtccgaaa aaaatgacgc agtgctccga tgcgcagttg ccatgtgctg ccgagcagat
     3541 gttgaagctc cagcggaaag gtcggcgtta gctggctacc atgcttagtc aggaggaaca
     3601 gggaaggggt ggcggcacgc aagagttcga gatcccggtt acggttcggt gccgtgtgcg
     3661 aggaaaggga gaccaggcga tcgcgggcgt cgaggtgatc gatgccttcc ggattggcaa
     3721 gggccagata ctgtcgcagg cgcgtcacgt gctggctgta tgccctcagc tgatcgagga
     3781 gcatcggatg gagccatacg attcgtgact gttcgtagct ctcgttatcc ttgtcgctca
     3841 cgaagcagag gcccgtcgca cggtcgatgt caaagtgcgc gggtagcaga ctcgatacgc
     3901 cacgcagtcc ggttgtcgcg agcaggaaaa acgcgacgta ggccgtatat gcgttgtgcc
     3961 tttcgacata tttcacaccg gcttctgatg ctagctttgc tactgcgttg aggacggtga
     4021 ggactgtagt ccgtacgctc gcccgcgttg gcacgtgtag cgagccaacg tgtgttgcgc
     4081 catattcggg agcacttgtt ggtgctgttg cggttgcggc aggataaggc agcccggcta
     4141 gctggtatag gcgcgcacaa gcgccggcgt agagcgtctg cagacggctc actgggacgg
     4201 ccgagtacac acaagggttg tgctgatttg ggggtatgcc tctgaagtag aattcgtaca
     4261 cgcggtcgtt cccgccaagc tgcgccatga atttggcgag caggcgcccg acgcggtccg
     4321 ggtgtagtgc cgtgccgcgg gtttggttga gggctgcgag tagctgcgcg aattcaggtt
     4381 cgtaggagcg ttgaaatagt ggttcctgag cgtgctcaac gcgactcagt gcagtggtga
     4441 gcgcgggcgg cacctccagc tcgaaccctt tggctgcggc gagggtgcga attgccggct
     4501 cgccctcaat gcgtggcgtg acatgctttg gggatgcgac agggagccag atgcgccctg
     4561 ttgtgggtgt ccacaagatg gttggcggaa cgcgggcgcg gttatggggc gccgtgcgta
     4621 ctttcctgat ttgcagcgtt ttgatcgctt ccggcgctct tgacaggaac agccggcatg
     4681 ccgcccaagc ggctacgagt ggcctgtcgg acgcggagat ttctccccag tcgtcagtgg
     4741 tcaggttatc gatcaggtcc actagcagtt ccatttcgta ccctgacagg gtggcgtgcg
     4801 taacggggag ctgctggtta gccagcgtga tcgcctgagc cgtgtatcgt gcaacattgc
     4861 gtgccaaagc tcgacgatcg cctctgttgg ccgcagatgc ggaccgtggc gcaacggcga
     4921 tcaacgttcc gcgtttgggc ccgtcgcctg gaggcgaaaa gtccagatcc cggtcgtgcc
     4981 gatgggtcga gccgtcatcc tcaacgaact gggtcacacc aatgtcaggc aaaccatcat
     5041 ccccttcgag cgttcgggaa atagaacgag agggctgagt cccgtcaatg gcgccgtgcc
     5101 gccccgcttt gtgcgtatcc gccctgccgg tctgcaggtg atgctggacc tcccaaacgc
     5161 cggtctcgaa ccactgcaat gcccgatgca ggtatccaag attcctgagt gtgccggtca
     5221 ccaagggtct aagacccagt cgtgacatgt ggttctcgaa gtgctcgagg agcgcttgcg
     5281 gatgcatcgg ttcgaggggg agttcgagca gaaatggccg caacgattcg tctggtcgat
     5341 cagtgagctg acgaagactg aggccgacca tgtacgcggg atcggccacg gtgttgatga
     5401 gtgtgcggtc ccgctccagc ctcatctgga cggggaacat tgataccagc agcgtaccgt
     5461 actgttgctc aagctcaggg tctgcacaaa cgtgccagaa aacgcagtaa tggggatgtg
     5521 aggcgtagta ccccttcagg attcttcgac cgcctcctaa ggcttccggg aaaaactgag
     5581 caacggagga agcaccgtca atgacctgct ccagtcccgc caggcacgca ctgatgagcg
     5641 gttgttgctt ttcgtcagta acgcgaacga gggaccgcaa gactggaacg agtctcctgg
     5701 ctacagccag aattccgatc aggtcgtccg gcatccttag gatgctcgtg agcggagcga
     5761 gcaggtggag gatctcctcg cggctattca tggaacgttg cctcaatcgc tggtgccatg
     5821 ttcgactggt ggcggctctg cttcctgaag aagatcaagc gtgacgcttt ttgccgcctt
     5881 gtccgtctca tcaatttttg agcggtttcg cgtggtgtgt cggtgcatcg cggtgcgcga
     5941 cattccgaca agccggccaa tcgcagcatc actcgtgaca tccacatagc ggccagcctc
     6001 ggagcgcaag acctgatcgc tcttcagcgc cgtggcgatg agcgcgagat cgtcgggatc
     6061 aggtctgcag atcagcttga tcagcagtgt gtccagcagc tccagttcat ctttgttggg
     6121 caaaggtgca tcgttggcaa cgcagagcgc ccacgtgagg tggtcacggg agtatttttc
     6181 caagaggagt tgcaaggttc gtcggccggc cagcaatatg tagcggcgtg cgtttgaggt
     6241 cgcaccaccg ggttcctcgc ggacgactag gggttgaagc agcggcagga gggataaggc
     6301 ctcctcccgc acggtcggga tgctctgagg caggtgccgc aaaccgacgc accacggtcg
     6361 agcgggcggt gggagatcga tctgattcag gcgtaacctg acggtccgag gaatatccat
     6421 ggtgtaggca tccggatggt ctagggcttc gcgcgacagc gaaaggtgtg cggcaactgt
     6481 accttggccg tgccattgag ccgcccgtca agcgtctcca cgaagacgtt gccaagcgag
     6541 gtactccgaa cggcgcaggc gatagcgggc gacgctgcct tcatgtaact gcatgagggc
     6601 cgcagtgagc agttctgcga aggccgctcg gtcggattct ggtgcgtttt ctgcggccag
     6661 tcgggcaatc accgcttgat tgggcggctg caggcctttg acgacggctt gtaccgcttg
     6721 gatcaagacc tcgcgatact tgatcttcag cggatcgggt tcgaccatgg tctggctgat
     6781 ggccaggtag cgctggcacg agcgctcata ggcccagacg aacacgtccc gcagaagctc
     6841 gatctggttg agttcgtaga cgcccagcgt tccttcgacg taggcgcgtt cggggacgtc
     6901 gatgaacgac agtggacaca ggttgtgctt gatcagcgag atgtttgcgc cgattcgcga
     6961 cacccgtttg ttcacatctt cgaatggctg caggtagggt agctgcacca tgacgaagaa
     7021 cgcctgctcg aaggggtccg ggatcgcagc ggtcttgccc agcaggaggg caaagcagtc
     7081 ttcgactacc tgcggcatgg ccaggggatg aaatacggtg ccggaaatat caacgggccg
     7141 tcgtcgcagc ctgccgcagg cgttcgggtt gcgcatcaga tcctgagaga gcacggagtg
     7201 gaggttcttg aacgtgaagg gatcgaagcc tatctcgtcg gcgttctcga tcagcatctc
     7261 gatggccgcc ttgtggttga ggatcatttg cgtctcaatg gcatccttgc cttgagcgac
     7321 ctgaccgtat tcgatcaggt tctgagtatc caggcgggta tatgtgttgc cttccagtct
     7381 ggatgaggcc caggacagat ccaccagcaa gcggccaagg atttcgcgag cgtaggtgcc
     7441 cgcagggcgc tcgccgaccg gggtgcgccc catttcgtga agctgacttc gcagcgactc
     7501 gggcagatag aacgtcgcgc ccggctgata ggcttcgagg aagtctcgcc gataacctac
     7561 tggtcggcgg tgcattagcg gcaatctcat ccgatcccgg attgcggccc cctcgggaga
     7621 gactggcacg tacaagtccg tctcgggtgc cttcacttcg tcggttcgag gctcggcatc
     7681 tgctgtcgat ttgtagacga gcgcgatgct ctcgccttcc ttcgtcagtc gccgcgcgtc
     7741 gatgagcttt tgcaggcgac gctgcagcgt ccgacggttg ggcctcatgc cctgacgacg
     7801 ctcgatctcg gcctcaatgg cggatatgcc caaccccttc gggtgatccg cgacgatgga
     7861 ctcgatgagc ttgagctctt ctgtaggggc aatccgcgac atcggtccgt tctccatcat
     7921 gcgttcggat ctgtaatgac cacaagagcg acaaagcgcg acaaggagcg acattattgt
     7981 gtcgctctaa atgtcgctgt tcaagagagc agtgctgcga tggtcagagg ggggggggtc
     8041 gtgcgtggcc gacgtttgtc agatgggcgt gtctttgctt tgacgaattg atcgaaggac
     8101 tgcctgatcc ttggctttcg agctccgtca cgttccatta tggaacatag agaattatac
     8161 ctcgggtacg cggagcaaag atgctgtatc gttgagacaa acgtaagcgc tccacgaacc
     8221 ccatccttcc ggggctcgct acgccgtgat caactgc
//
LOCUS       DAOQEW010000169         8243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2983_length_8243_cov_9.103200, whole genome shotgun sequence.
ACCESSION   DAOQEW010000169 DAOQEW010000000
VERSION     DAOQEW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_8243_cov_9.103200"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLS35_15295"
     CDS             complement(<1..922)
                     /locus_tag="PLS35_15295"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNI20088.1"
                     /translation="MKVFTHTRSTTLMYDGTEQDLLCLLASRAGISPEYYDIAGTLHV
                     TSDDTRRAILSAMGFDTTSRESLATTLADWDEASWRQPCDPILILEQGYGPTRWAFRL
                     PLEEKEEAQLVLTWHLCDEKGTVVHREDAGPRLIPQEVHVAAGRRMVRLDLPLVPNLA
                     LGYYDLSVVGTVGTLVTKAVLRVVVAPPHCYVPPGMAQGQRVWGLAVQLYALRSRTNW
                     GVGDFTDLARLVEWAGKELGAGIIGLNPLHALKNSRPHHLSPYSPTSRLFLNELYIDL
                     DRLPEYHASPEAQRLRQSPEFQSGVEQARAA"
     gene            981..2003
                     /locus_tag="PLS35_15300"
     CDS             981..2003
                     /locus_tag="PLS35_15300"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNI20089.1"
                     /translation="MSVDQASLQDRCPAARDSTVPFQAIYSMSNRLLVRFGTSSWAYE
                     GWRNQIYHRPYAASRFSKDSLAEYATYPTGGTPLFRTVGIDHSFYRPASATQLAHYAS
                     QVPADFHFCSKVWEELTVPAYADLPRYGAKAGKPNPRFLDSHLFREMVLQPFLEALPG
                     HIGPFIFEFQRTGMDPVVFLDALDRFLADLPAGFPYAVEIRNPAVLSPRYRDILLAHQ
                     VAHTYNHWTGMPPLLTQHRLLNERFTAPFVVMRLLTPLGLPYADAVERYAPYNRIVTP
                     QPQMRREAASLIHSASSQGVTPYVLVNNRSEGNAPLTIQAIVEMVTALPDSPALLPSP
                     SRDSMI"
     gene            2065..2796
                     /locus_tag="PLS35_15305"
     CDS             2065..2796
                     /locus_tag="PLS35_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20090.1"
                     /translation="MTPLLSMFRSGAVFAVAVHCLAPAIPVQAASDHSRVDITGDYRY
                     AYHQPETASEAKQHACREALHQAISTSTAVREHTASIVDSKLFHTLVHTLAAQHVTDQ
                     QILQQSEQGRTVYCKIKAVFQADAVERVLLAQTTSGSEQALDQNRALRILAAHEEPDG
                     MLVITYQAIKRLDWLGTAYQGGLRESADVMVDFYDEQGILVRSSRHPARKTATGDDVM
                     NPGEVGTLKVAKPLNGKTFRVWVVK"
     gene            complement(2879..3706)
                     /locus_tag="PLS35_15310"
     CDS             complement(2879..3706)
                     /locus_tag="PLS35_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNI20091.1"
                     /translation="MRQGAWSVLGIMGVMLVTSGCVAIEAGHEGVMVEQPFFFGHGGV
                     DPAPTKTGRVWVAPTTKVIEVDIRPLQYSEHFDIISAENAPVSFDAFLIANVVEGRTP
                     ELIGRYGTTWYQNNVKEAFRTFVREEVQRYPLFQLTTDPTTRTKLQDAIAREVQNKLI
                     DKQNMPIRLNRVVVGSILPPKGVVEQTTQTIVQEQRKITMVEFQKAEEAREKAEKQRG
                     IADRAYRESLGLTAPEFVDLRRIEVQKEIVQHAPSALTVIMGLERVGINMPSSGADR"
     gene            complement(3891..4313)
                     /locus_tag="PLS35_15315"
     CDS             complement(3891..4313)
                     /locus_tag="PLS35_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI20092.1"
                     /translation="MNLNRSTHIRMKAWMAGIGLVVLVAALPACESNATNQDAGKPAG
                     GATPADMQVGEAKFSANCAVCHGVRGVGTKQGPPLVHKIYEPNHHADMAFQRAAENGV
                     RAHHWEFGNMPKIEGVTPADVEQIIRYVRWLQREAGIY"
     gene            complement(4310..4486)
                     /locus_tag="PLS35_15320"
     CDS             complement(4310..4486)
                     /locus_tag="PLS35_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20093.1"
                     /translation="MTPEEFFMYLIIALVIIAPIGARLYRRLTLNRTTQMLREQLHSS
                     QSKPSSTTPPELPR"
     gene            complement(4531..5229)
                     /gene="queC"
                     /locus_tag="PLS35_15325"
     CDS             complement(4531..5229)
                     /gene="queC"
                     /locus_tag="PLS35_15325"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNI20094.1"
                     /translation="MTPLQPKAVVLLSGGLDSTVTAAIAQQDGYSIHCLTVSYGQRHQ
                     VEVERARAVAAALGTAGHVVVTVDLRAIGGSALTADVDVPKDRPPEARASEIPVTYVP
                     ARNTIFLSLALAYAETVKASAIYFGANVLDYSGYPDCRPDFIRAFETVARLGTKMGVE
                     GHGAEVRAPLLMMSKADIVRRGQALNVPFELTHSCYDPGVEGLACGRCDSCRIRREGF
                     RLAGVVDPVPYAIL"
     gene            complement(5350..5925)
                     /locus_tag="PLS35_15330"
     CDS             complement(5350..5925)
                     /locus_tag="PLS35_15330"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNI20095.1"
                     /translation="MATKVGAKKKTPTTKAKAIAPEKPVKKERPAPVTTMTEKDEDSV
                     AARVVAALTLKETPKEKEERQRRREVLQRMLLGKRQEIMREIEGNLGQSLTEDQQRRL
                     ESARDVGDQALMDLDRELGISLMEMRNRKRQAIDEALTRLSEGTYGICAECGIEVSEK
                     RLEAVPFAKLCVQCQSRQELLEKIEKEEDRD"
     gene            complement(6031..6546)
                     /locus_tag="PLS35_15335"
     CDS             complement(6031..6546)
                     /locus_tag="PLS35_15335"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNI20096.1"
                     /translation="MNYKSLIREVPDFPKPGILFYDITTLLKDPQAFRSIADELAARY
                     EGQGIAKVIGIESRGFILGGTLAARLGAGFVPVRKPGKLPADIYEVEYNLEYGSSVLA
                     IHRDAVSPGERVLIVDDLLATGGTAAATVDLVGQLGGEIAGLDFLIELKALKGRDRLT
                     GFNVHSLIIYP"
     gene            complement(6571..7569)
                     /locus_tag="PLS35_15340"
     CDS             complement(6571..7569)
                     /locus_tag="PLS35_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNI20097.1"
                     /translation="MARRATVRQDSEVGAPVSTRRPRAARAEEDEDAESSGLAESEDS
                     GGESRQAESGRAEGLDTIKSYLREVRKSTLLNFKQEQALGKRVMAGDEAARQEMIEAN
                     LRLVISIGKRYMNRGFPFADIVEEGNLGLIKAVEKFNYKRGFRFSTYASWWIRQYIER
                     AIINQGKLVRLPVHVVERLNRYLGKVEQLVHELGREPLAEEVAAKLKTSVAEVDDLKQ
                     LVRTTCSLDSPISDRQDTFLRDVIEDPLCLTPADTTEGVMRRAELMAWVNELPEKERT
                     VILARFGLDGAESRTLEEIGHEMGLTRERVRQIETAALARLRGIIERKTMKRADLL"
     gene            complement(7584..7871)
                     /locus_tag="PLS35_15345"
     CDS             complement(7584..7871)
                     /locus_tag="PLS35_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNI20098.1"
                     /translation="MTEIGESVRARILVSGRVQGVGYRAFTCRMAAARGLTGGVENLD
                     SGQVAVDAEGGRAVLEEFLVDLKKGPVGARVTQVQVEWGNATGRYHDFSIW"
BASE COUNT         1653 a         2591 c         2288 g         1711 t
ORIGIN      
        1 ctgccgcgcg tgcctgctcc acgccgcttt gaaattcagg gctctgtctc agccgctggg
       61 cctcgggcga tgcatggtat tcaggcaggc gatctagatc gatatagagt tcgttcaaaa
      121 agagacggct tgtcggagaa taaggactga ggtggtgagg gcgcgaattc ttgagcgcgt
      181 gtaagggatt caacccgatg atcccggccc ccaattcctt gcccgcccat tcgaccaggc
      241 gggccaaatc ggtaaaatcg ccgacacccc aattcgtgcg ggaacgcaat gcatagagtt
      301 gcaccgccaa tccccacact cgctgcccct gcgccatccc tggcggcacg taacaatggg
      361 ggggagccac cacgacacgg agcaccgctt tcgtgaccaa ggttccgact gtgccgacca
      421 ccgaaagatc gtaatagccc agcgctaagt tcggtaccag cggcaagtcg aggcggacca
      481 ttcgccgacc ggccgccacg tgcacctctt ggggaatgag tcgcggaccg gcatcctcgc
      541 gatgaacgac tgtccctttt tcgtcgcaga ggtgccatgt caggactaac tgcgcctctt
      601 ctttctcctc caggggaaga cgaaaggccc atcgagttgg accatacccc tgttcgagaa
      661 tcagaatcgg atcgcagggt tgtctccacg acgcctcatc ccagtcggcc aaggtagtgg
      721 ccaacgattc tcgcgacgtc gtgtcgaacc ccatggcaga aaggatggcg cgacgcgtgt
      781 cgtcgctggt cacatggagt gtcccggcga tgtcgtaata ctcgggggaa ataccggccc
      841 gcgaggcgag gaggcacagg aggtcttgct cggtaccgtc gtacataagt gtcgtggacc
      901 gagtgtgagt gaagaccttc atcaagtcaa ggcgtcctac gcttttccgc agggttgcga
      961 tacaatcccc gcatgaacag atgtctgtcg atcaggcctc tctccaggac cggtgcccgg
     1021 ctgcccgcga cagcaccgtt cccttccaag cgatctactc gatgagcaac cggctgctgg
     1081 ttcgttttgg caccagttca tgggcctatg aaggctggcg gaaccaaatc tatcaccggc
     1141 cctatgcggc cagccggttc tccaaggata gtctggctga gtatgcgacg tatccaacag
     1201 gcgggacgcc gctctttcgc accgtcggga tcgaccattc cttttatcga ccggccagcg
     1261 ccacccagct cgcgcattac gcaagtcagg ttccggctga tttccacttt tgctcgaagg
     1321 tgtgggaaga actgacggtt cccgcctatg ccgatcttcc acgatatggc gccaaggccg
     1381 gaaaacccaa ccctcgattt ctcgatagcc atctctttcg cgagatggtg ttgcagccgt
     1441 ttctcgaagc ccttcccggt cacattggcc ccttcatctt tgagtttcag cgaaccggaa
     1501 tggaccccgt cgtcttcctc gatgcccttg accgtttcct ggccgacctg ccagccgggt
     1561 ttccctacgc cgtggagatc cgaaatcctg ccgtcctgag ccctcgttat cgggacattc
     1621 tcctcgcaca ccaggtcgcg cacacctata accactggac cggaatgccg ccgctgctga
     1681 cccagcatcg tctcctcaac gaacggttca cagccccatt tgtcgtgatg cgcctcctca
     1741 ctccgctcgg tctcccctac gccgacgccg tggagcggta tgccccgtac aatcgcatcg
     1801 tcaccccgca gccgcaaatg cgccgggagg ccgcctcgct gattcactcc gcttcctccc
     1861 aaggcgtgac gccttacgta ttggtcaata accgctcgga aggcaatgca ccgttgacca
     1921 tccaagcgat cgtcgagatg gtgacggcgc ttccggactc ccccgcattg ctgccgtctc
     1981 cctcgcgtga tagcatgatc tgacgtgagc accgccctga acctgcacgc accagaatcc
     2041 gacaggaaag aggaggtatc tcccatgacc ccgctgctgt cgatgttccg aagcggcgcc
     2101 gtcttcgcgg tcgccgttca ctgcctggcc ccagccattc ccgtccaggc cgcatcagac
     2161 cactcccgtg tcgacatcac cggcgattat cgttacgcct atcaccagcc cgagacggca
     2221 tcggaggcca agcagcatgc ctgcagggaa gcgctgcatc aggccatcag tacctcgacg
     2281 gccgtgcgtg agcataccgc ctcgatcgtc gactcgaaac tgttccacac gctcgtccat
     2341 accctggctg ctcagcatgt caccgatcaa cagatccttc agcaaagcga acagggccgc
     2401 acggtgtact gcaagatcaa ggcagtcttc caagcggacg ccgtggagcg agtcctcctg
     2461 gctcagacca cgagcggatc agaacaggcc ctggatcaga atcgggcgct gagaattctg
     2521 gccgcgcatg aggagccgga cgggatgctg gtgatcacct accaggcgat caagcgtctc
     2581 gattggcttg gcacggccta tcagggagga ttgcgcgaat cagccgatgt gatggtggat
     2641 ttttacgacg agcaagggat cctggtgcgc agcagccgcc accccgccag aaaaacggcg
     2701 acgggtgatg acgtgatgaa ccccggcgag gtggggaccc tcaaagttgc gaaaccgctg
     2761 aatgggaaaa ccttccgtgt gtgggtcgtg aagtaacccc caaaatgcag gaagcaaaac
     2821 aggatccccg gttttgcttc ccgtttcgcg atacccctga atcagcggca cgaattcgtt
     2881 agcgatcggc gccggacgac ggcatattga tgccgactcg ttccaatccc atgatgaccg
     2941 tcagggctga cggcgcgtgc tgaacaattt ccttctgcac ctcgatccgg cgaagatcaa
     3001 caaactccgg cgcggttagt cccaacgatt cccgataggc ccggtcggcg atcccgcgct
     3061 gcttctcagc cttttcgcgc gcctcttcgg ctttttgaaa ctccaccatc gtaatctttc
     3121 gctgttcctg cacgatggtt tgcgtggtct gttcgactac ccccttcggc ggcagaatgc
     3181 tgccgacgac cacgcgattg agtcgaatcg gcatattctg tttatcgatg agcttgttct
     3241 gaacctctcg cgcaatggca tcctgcaact ttgttctggt cgtcggatcg gtcgtcagct
     3301 gaaagagcgg ataccgttgc acttcctcac gcacgaaggt gcggaacgct tctttcacat
     3361 tgttctggta ccaggtggtg ccatatcgcc cgatgagttc aggcgtcctc ccttcaacga
     3421 cgttggcaat caggaaggca tcgaaggata ccggagcatt ttctgcggag atgatatcga
     3481 aatgttcgga atactgtaat ggacggatat ccacttcgat caccttcgtc gtaggtgcca
     3541 cccacacccg tccggtcttg gtcggcgccg gatccactcc gccgtggccg aagaagaatg
     3601 gctgttccac catgacgccc tcatgaccgg cctcgatcgc cacacagcct gaagtcacca
     3661 gcatcactcc catgattccg agcacggacc aggctccctg tctcataggt tcctccaatg
     3721 aaaaggtccg accaccgagc cgaattccct gagcggggga actcacggcc acgtgttgtc
     3781 gtgcggatac cttacgaacc gcgtgggcgg ttgtcaaacc aggcttgagg atgaagagaa
     3841 aacgagaaaa gtgaggagaa gcgcaaccaa acggacggca agtcggccgg ctagtaaatt
     3901 ccggcctcgc gctggagcca ccggacgtat cgaatgatct gttccacatc ggccggcgtc
     3961 accccctcga tcttcggcat attgccgaac tcccaatggt gggctcgaac accgttttcc
     4021 gccgcgcgct ggaacgccat atcggcatga tggttcggtt catagatctt gtggaccaac
     4081 ggaggtccct gcttggtgcc cacaccacga acaccgtggc agacggcaca attggcgctg
     4141 aacttcgcct cgcctacctg catatcggcc ggtgtggcgc ctccggcagg cttgcctgca
     4201 tcctgattgg tggcgttcga ttcgcaggca ggcagggccg cgacaaggac gaccaagcct
     4261 attcccgcca tccaggcttt catccggatg tgtgtggatc tattcaagtt catcggggta
     4321 gctccggcgg agtcgtcgag gacggcttgg attgtgaaga atgcaattgc tcgcgcaaca
     4381 tctgcgtggt gcgattcaac gtcaaccgcc ggtagagccg ggcgccgata ggcgcaatga
     4441 tcaccagcgc gatgatcaga tacatgaaaa attcttcagg cgtcatgggc ccatctctcc
     4501 tgactcatag gcaaccgcac caaccgttcg ctagagtatc gcatagggga ccggatccac
     4561 aactcccgct agacggaatc cttcccgccg aatccggcaa ctgtcacacc ggccgcaggc
     4621 gagcccctcg acgccaggat cgtaacaact atgcgtcaac tcgaagggaa cgttgagcgc
     4681 ctgcccgcgc cgcacgatgt ccgccttgga catcatcagc aggggcgctc gaacctcggc
     4741 cccatgcccc tcaaccccca tcttggttcc aagccgggcc acggtttcaa acgcgcgaat
     4801 aaaatcgggg cggcaatccg ggtatcccga atagtccagg acgtttgctc cgaagtagat
     4861 ggccgaggcc ttcactgtct ccgcataggc caatgccagc gagaggaaga tggtattgcg
     4921 ggcgggaaca taggtcacag ggatctcgct tgctcgtgct tctggcgggc gatccttagg
     4981 cacgtccaca tccgccgtga gggccgaccc tccgatggcc cgtagatcaa ctgtgaccac
     5041 cacgtggccg gcagtgccta gcgccgcagc aacggcacgg gcccgttcga cctccacctg
     5101 gtgacgctgt ccataggaga ccgttagaca gtgaatgcta tacccgtcct gctgcgcgat
     5161 ggcggccgtc acggtggaat ccaagccacc gctcaagagg accacggcct tcggctggag
     5221 aggagtcata gacgcgggga agaataaggg ggaatcgcga agccggtcac aggtgagcga
     5281 gtgaccggct tctcatatcg atgcattccg catacatgcg gtggaaacgg aaggggttgc
     5341 ccgattcggt tagtcccgat cctcttcctt ctcaattttc tcgaggagtt cctggcgaga
     5401 ctggcattgc acgcacaact tggcgaaggg caccgcctcc aaacgcttct cactgacttc
     5461 gatgccacat tctgcgcaga taccgtaggt cccttcgctg agccgcgtca gcgcttcgtc
     5521 gatggcctgg cgcttccggt tccgcatttc catcagagaa atgccgagct cgcgatccag
     5581 atccatgagc gcctgatcac ccacatcgcg ggccgactcc agccggcgct gctgatcttc
     5641 ggtcagcgac tggccgaggt tgccctcgat ctcgcgcatg atttcctggc gcttgccgag
     5701 cagcatccgc tgcaggactt cgcgccggcg ctgacgttcc tctttctctt tcggcgtttc
     5761 cttaagcgtc aacgcggcca ccacgcgtgc ggccacgctg tcttcgtctt tttcagtcat
     5821 agttgttaca ggagccggtc gctctttctt cacgggtttt tcaggagcga tggcttttgc
     5881 tttcgtcgtt ggtgtttttt tcttcgcgcc gactttcgtc gccatagagt ctcatgccct
     5941 ttcgtgatga gtgcaaggcg aaaaagtttg gcaggtatat cacgctatag atcgtttgac
     6001 aagtacccgc ttccccaaga tcttggggat ttatgggtaa atgatcaggg agtgcacgtt
     6061 gaacccggtc aggcggtcac gtcccttgag ggctttcaat tcgatgagga agtccaggcc
     6121 ggcaatttcc ccgccgagtt gaccgaccaa gtcgacagtg gccgcggccg tgccgcctgt
     6181 ggccagcaaa tcgtccacga tcaggacacg ctctccgggc gatacggcat cccgatggat
     6241 cgcaagcacg ctggatccgt attcgagatt gtattcgact tcatagatgt cggcgggcaa
     6301 tttaccgggc tttctcaccg gcacgaaccc ggcgccaagt cgcgccgcca gcgtaccgcc
     6361 gagaataaac ccgcgcgact cgatcccgat gaccttcgca atgccctgcc cctcgtaacg
     6421 agcggccagc tcatcagcga tggatcgaaa ggcctgtgga tctttcagaa gcgtggtgat
     6481 gtcgtagaag aggatgccgg gcttaggaaa gtcgggaact tcgcggatga gggacttgta
     6541 gttcatgggc atgatggaat cgggacgttc tcataaaaga tccgcccgct tcatggtttt
     6601 ccgttcaatg atcccgcgga gtcgtgcaag cgccgcggtt tcgatttggc ggacccgctc
     6661 gcgagtcagc cccatctcat ggccgatctc ttctaacgtc cgcgactcgg cgccgtcaag
     6721 tccaaaccgc gccaaaatca ccgtacgttc cttttcgggc aattcattga cccaggccat
     6781 gagttccgcc cgtcgcatca caccttcggt cgtatccgca ggcgtaagac agagcggatc
     6841 ttcgatcaca tcgcgcagga aggtgtcctg ccggtcgctg atcgggctgt cgagtgaaca
     6901 ggtggtgcga accagctgtt tcagatcgtc cacctcggcg acactggttt tcaacttggc
     6961 cgccacttcc tcagccagcg gttcccgacc gagttcatgg accaattgtt ctaccttacc
     7021 gaggtaccgg ttcaaccgtt cgaccacgtg aaccggcaag cggacaagtt tgccctggtt
     7081 gataatggcg cgctcgatat actgacgaat ccaccaggac gcgtacgtac tgaagcggaa
     7141 tccgcgcttg taattgaact tctctacagc cttgatcagg ccgagattac cttcttcgac
     7201 gatatcggcg aacgggaatc cgcggttcat gtaacgcttg ccgatactga tgaccaggcg
     7261 gagattcgct tcgatcatct cctgacgcgc agcttcatcg ccggccatga ctcgcttgcc
     7321 cagcgcttgc tcttgcttga agttcagcag ggtcgacttg cggacctcgc gcaggtaact
     7381 cttgatggtg tcgagacctt ccgcacgccc ggattccgcc tggcggcttt ctccgccgga
     7441 atcctccgac tcggcgaggc ctgacgactc cgcatcctca tcctcctccg cccgcgcagc
     7501 acgcggacgt cgggtcgaga ccggcgcacc tacctcgcta tcctgtcgca cggttgcacg
     7561 tcgagccata acgtcgtcac ctactaccaa atcgaaaaat cgtgataccg gcccgtggcg
     7621 ttgccccact ccacttgtac ctgggtcaca cgcgctccga ccggcccttt tttgagatcg
     7681 acgaggaact cctcaaggac ggcccgtcca ccttccgcat ccaccgcgac ttgtccgcta
     7741 tcaagatttt ccactccgcc ggtcaacccg cgcgcagcgg ccatgcggca ggtgaaggct
     7801 cggtagccca ctccctggac acgaccactc accaggattc gcgcgcgcac agattcgccg
     7861 atttcggtca tcgtcagtac gtcctcaatg ccagggtgtt ggcgcagagc gatttccccg
     7921 cctagcacag aatactctca cagcgcctac aggtcgtcac gcgatttgca actttacgcg
     7981 gctgtctcag gctcatacgc ctaccttatc ggcaggaccg tcacaaacca tgagcgccat
     8041 gacacacctc gccggtttgc ggccgaacgt cgtcacgttc agcgcgactg gagaggcact
     8101 tggtcgatgg aacgctgcga ggaattacta attggagcgc ttggcccgaa gaactgggaa
     8161 agaaagtctg acgggagaag gaagtgctgt ttggtcgggg cgagaggatt tgaacctccg
     8221 acccctgcgt cccgaacgca gtg
//
LOCUS       DAOQEW010000170         8226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2992_length_8226_cov_7.082120, whole genome shotgun sequence.
ACCESSION   DAOQEW010000170 DAOQEW010000000
VERSION     DAOQEW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2992_length_8226_cov_7.082120"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1198
                     /locus_tag="PLS35_15350"
     CDS             65..1198
                     /locus_tag="PLS35_15350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNI20099.1"
                     /translation="MGIPFYRHDLGEPEVEAVRKVLAGPILTTGEVVESFERRFAAYL
                     GVPHALAVTSCTGALHLSLLALGIGLGDEVITTPMTFIASSTAILEAGAKPVFVDVES
                     QTGNLDATKVEAAITPRTKAILPVHLYGQMCDMVTIRKIADQHGLFVIEDAAHCLEGV
                     RDGVKPGELGDTACFSFYATKNLTCGEGGALVTKDRGLAEKLRLLRLHGMTKTAADRH
                     REGYQHWDMTVLGWKYNMDNIHAALLLPQMDRIEQNWSKRDTVARWYEGLLSDCPGVS
                     VPKSHPQVRHARHLFPVFVEGGGRDAIIQGLQEDGIGSVVNYRAIHLLSYFRDTFGFR
                     VGDFPHAEKIGDSVVSLPFYPAMTENQVTQVTATVTRLLKARR"
     gene            1314..2636
                     /locus_tag="PLS35_15355"
     CDS             1314..2636
                     /locus_tag="PLS35_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNI20100.1"
                     /translation="MSTLKLSLVGLGARVILLVINVISVVLLARYLEPQGRGEYFLFQ
                     TLVSVLSVVGDCGLSQSANVFVGRSAHRKKLIHAFVSKAAIILWIGMSGIGGLVLWMA
                     GEQLLHDFPARWQWAAFAILPCMLYAGFWNSLMIGLGEIPLLNGVQLVIGPVQLILIL
                     VLVVGFSGGVASAVTIYLSTMVLQCVLMVALSRRLGFLGGSEYVEAGLSRQMLAFGLR
                     AYPNAIATMGWMRIPVFVLNAFHGAASVGIFSVAQQLAERALMPIQAAQDAIYGKMSA
                     LSPREAIAAMNRYIRIVVSGMVASTVGAMLLAPWIVMALFGPSYQPAVYVFWILLVGV
                     VFVSVSMIVSTYVLGQRGRPGLLSLLAGANACICLASSYWLIPTWAEMGAAMALALTQ
                     AVGAVVVFGLYWITSSATLRETLLLTSEDVTSVLKQLGKLTWRKGTQS"
     gene            2705..3874
                     /locus_tag="PLS35_15360"
     CDS             2705..3874
                     /locus_tag="PLS35_15360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20101.1"
                     /translation="MWDIVRLSEPTLCKHFVISFFPDNAKFVYSEPLRSRGAYKQPAH
                     HPFLEWLERGIQTVASQWYLLPIRKTLSGLWWFCNAVDVWWRVAVALVQKRPDVIHVH
                     TFHGLRVGLVLRRMLQIPLVHSVPALFSQMTDAGFHGIPQLYRSNQQWVARFITGASV
                     AELHSIGIPDSKIMPIHGTVDLTSSTSMRKQKQHYYTALRDELKLPGDAVIALSVGRL
                     HPSKGHAFALEAVPELVAQFPQLHWVVLGEGEQREALEARASELKVREHVHLLGFQRD
                     PLPYYAAADIYLRTPVFESENLSSYQAMAMGLPVVGFDTGCETELLGKSGHGVLVPAR
                     NSEALAKAVVEMLSLPDRGARIGLRGVEYCRQHLDIDRTISDLVRLYQHLGAKRA"
     gene            3932..5026
                     /locus_tag="PLS35_15365"
     CDS             3932..5026
                     /locus_tag="PLS35_15365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20102.1"
                     /translation="MKVTFVISALFAGGAERVISIMANYWARKGWGVTILSFDDGETP
                     PFFEVDPSVRRMSLGLLGDSAGLIAGIRNNINRVVRLRQAILRSQPDAVISFVDKTNV
                     LTLFATLGLKLEVMVSERTNPYQYSIGRIWGALRWWVYSRADRIIVQTHAARAYFLPK
                     FQRTVSVIPNPVLPSSSNYAMVNHRPAGCMILAMGRLSKEKGFDDLLYAFRKVNDQHP
                     GCTLTIIGEGPERGELELLCERLGLSGCVLLPGLVTTIEVFLRQASMFVLSSRFEGFP
                     NALCEAMAYGLPVIATDCRSGPREIIRDGEDGLLVPPGDRDALATAMDSLMSDPERRA
                     RLGGRAMDITQRFGVEKVMGMWEKILEPIA"
     gene            5040..5882
                     /locus_tag="PLS35_15370"
     CDS             5040..5882
                     /locus_tag="PLS35_15370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI20103.1"
                     /translation="MMTQQAQEVADGRRFEFGQNWRRFLEDLTEERIGRAQESLQAML
                     GLKSLAGQRFLDIGSGSGLFSLAAKRLGASVRSFDYDPHSAACTAELKRRYFRDDDTW
                     IVEEGSILDMAYVQSLGTYDIVYSWGVLHHTGAMWQALDNASRLVADQGTLFIAIYND
                     QGGASRRWLMVKRVYNRSPQWGKILVAWLACIRQWTLTVVRDTFLGNPLRTWFSYKEQ
                     RGMSPWRDVIDWVGGYPFEVAKPEEIFDFYRKKGFDLQRMKTCAGGIGCNEFVFHRVS
                     TERL"
     gene            5879..7003
                     /locus_tag="PLS35_15375"
     CDS             5879..7003
                     /locus_tag="PLS35_15375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20104.1"
                     /translation="MKVRFLIRSLQRGGAERQCVALAKGLRQLGHDVGVLVFYSGGDL
                     YDELLAAGIPVESVGKLRRWELVGFLQRIRRCLRRERPDVVYSLLQVSNVVAAVEKLF
                     VPTLRVTWGIRTASMDLTHYDRLVRVSYWLEARLSRFADHIIVNSEAGLRYAKVEGYR
                     TDRISVIPNGIDTELFAPDRSKGKTVRCSWGIRDEEVLIGLVGRLDPIKDHPTFLRAA
                     ALVVRQCALVKFVCIGGGDSAYKESMVALCAELALSARVIWAGEHSDMPAVYNALDVA
                     CLTSTAEGCPNTLLEAMACGVPCVATDVGDSKRILGEIGGIVPSRNSEALAGAIQELL
                     RMDGAERAKQGQMGRDRVIQHFSLDRLIGDTASVLEATAR"
     gene            7170..>8226
                     /locus_tag="PLS35_15380"
     CDS             7170..>8226
                     /locus_tag="PLS35_15380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20105.1"
                     /translation="MIRIIHVISGLDTGGAEVMLVKVLQGLDRTRFTNIVISLTDRGQ
                     LGEQIEASGIALHTLGMKRGCPNLSALVRLRRLFTTLQPAIVQSWLYHADLLSTLAVK
                     VSGSPILIWNLRCSHMDLTGYSRLTRIVQWMLVWLSHVPVAVIANSVAGRQQHARLGY
                     RPRRWVVIPNGFDLQRFRPDRSARAAIRTEWGLSDDTVLVAFIARVDPMKDHATFLDA
                     AKRVVDAKKKAHFVLVGKDTETLAPSVAKRGLTDHVLLLGHHGHIERLLPGVDVGCLS
                     SLGEGFPNVLGEAMACGIPCISTDVGDARDIIGDTGLVVPLRDPAALAGAMTDLIDRG
                     PAGRAKLGYAARARIEAE"
BASE COUNT         1619 a         2044 c         2552 g         2011 t
ORIGIN      
        1 ccgcagatgt ggatgagacg gattcccatc gtcaccgcgg cgggtaagcg ggagctacac
       61 tgagatgggc attcctttct atcgccatga tttaggcgaa ccggaagttg aggccgtgcg
      121 aaaggttctc gcggggccaa ttttgacaac aggggaagtc gtcgagtcgt ttgagcgacg
      181 ctttgcggcc tatcttggtg taccgcatgc gctggccgtg acgagttgta ccggtgcgct
      241 tcatctttcc ctcctggcat taggcatcgg ccttggagac gaagtgatca cgacgccgat
      301 gacctttatt gcgtcctcca cggccattct cgaagccggg gcaaagccag tttttgttga
      361 tgtggaatcg cagacgggca atttagatgc gaccaaagtc gaggcggcga ttacgccacg
      421 gacaaaagct attctgcctg ttcacctgta cggacagatg tgcgatatgg tgaccattcg
      481 caaaatcgct gatcaacatg gcctgtttgt gattgaagat gccgctcatt gccttgaagg
      541 tgttcgtgat ggggtgaagc caggtgagtt gggagatacg gcgtgcttct ccttttatgc
      601 gaccaagaat ctgacctgcg gagagggagg ggctcttgtg accaaggatc gggggctagc
      661 cgaaaagctt cgcctgctca ggctccatgg catgacaaaa accgcagcag atcgtcaccg
      721 cgagggttat cagcactggg acatgaccgt gctgggttgg aagtacaaca tggataatat
      781 ccacgccgcg ctcctccttc ctcaaatgga tcgtatcgaa cagaattggt ccaagcgtga
      841 taccgtggcc cggtggtatg aaggcttgct gtcggattgc cctggtgtgt cggtgccaaa
      901 atctcatccg caggtgcgtc acgccaggca cctctttccc gtgtttgttg agggcggggg
      961 acgagacgca atcatccaag ggttgcagga ggatggaatt ggcagtgtgg tgaattatcg
     1021 cgcaatccat cttctgagct actttcggga cacattcggg tttcgtgtcg gcgatttccc
     1081 gcatgccgaa aaaattggcg atagcgtggt gtccctcccg ttttatccgg cgatgaccga
     1141 aaaccaggtc acccaggtca cggcaacagt gacgcgcctc ttaaaggcgc ggaggtgagc
     1201 cttcaatggt cctgcatagc ctcagtttct gtgcatacct gagtgtgtaa tccgactgtc
     1261 cgcacccagg aacccgagtc caggcgccac agcagctctt cgggatcacc cgcgtgtcta
     1321 ccctaaaact ttcactggtc gggcttgggg caagagtgat tctcctggtc atcaatgtga
     1381 tcagtgtggt tctgcttgcc cgatatttgg agcctcaagg gcgcggtgaa tattttctct
     1441 ttcaaacgct cgtgtcagtg ctcagcgtgg tgggtgattg tggactctca caaagcgcca
     1501 atgtgtttgt cgggcggagt gcccatcgca aaaagctcat ccatgcattt gtcagtaagg
     1561 cggcaatcat cttgtggatt gggatgagtg gaatcggtgg gttggtgctg tggatggccg
     1621 gtgagcagct cctgcatgat tttccagcgc gctggcaatg ggcggcgttt gcaatcctgc
     1681 catgcatgct gtatgcgggg ttctggaata gcctcatgat cggattagga gaaattccgc
     1741 ttctcaatgg cgtgcaactc gtgataggtc ccgtacagct cattctcatt ctcgtgcttg
     1801 tcgtggggtt ttccggagga gtggcctcgg ccgtgacaat ctatctctct accatggtgc
     1861 tgcaatgcgt gctcatggtg gcgctatcgc gtcgtttggg atttcttggc ggaagcgagt
     1921 atgttgaggc gggcttatcg cgacagatgc tggcattcgg tcttcgtgcc tatcccaatg
     1981 ccattgcgac catggggtgg atgcgtattc ccgtgtttgt gctcaatgca tttcacggag
     2041 ccgcttcagt ggggattttt tccgtggcgc agcaattggc ggagagagcg ttgatgccca
     2101 tccaggccgc gcaagacgcc atctatggga aaatgtcggc gttatcgcca cgcgaagcga
     2161 ttgcggcaat gaatcgctac atacgaattg tggtgtctgg gatggtcgcc agcacggtgg
     2221 gagctatgct tcttgcaccg tggattgtga tggcgctgtt tggaccatcc taccagccgg
     2281 ccgtgtatgt gttctggatc cttctcgtcg gagtggtatt tgtgagtgtg tccatgatcg
     2341 tcagcaccta tgttctgggc cagcgagggc gtccaggtct cctctctctg ctagctggag
     2401 cgaacgcgtg catttgtctg gcgtcgagtt actggctaat tccaacatgg gcggagatgg
     2461 gagccgccat ggctttggcg ttgacccaag ccgtaggggc tgttgtggtg ttcggtctct
     2521 attggataac ttcctctgca accctacgcg aaacactctt gctcacatcc gaggatgtca
     2581 cgagtgtgtt gaaacaactg ggtaaattga catggcggaa gggcacgcag agctaacatc
     2641 agccgatccc atcagcgtgg tgcaccttat cgacggactg ggcggcggcg gttccgagcg
     2701 gtgggtgtgg gatattgtcc gtctttctga gccgactctg tgcaagcatt tcgtgattag
     2761 cttctttccc gataacgcaa agttcgtcta ttctgagccg ttacgcagtc gaggcgccta
     2821 caagcaaccc gcgcaccatc ctttcttgga atggcttgaa cgaggcatcc agactgttgc
     2881 ctcccagtgg tacctcttgc ctatccgtaa gacactttcc ggactttggt ggttttgcaa
     2941 tgcagtggat gtgtggtggc gggtcgctgt tgcgctcgtt cagaaaagac cggatgtcat
     3001 tcacgtgcat acgtttcatg gccttagggt gggattagtc ttacggcgaa tgttgcagat
     3061 accccttgta cattctgtcc cagccttgtt ttcgcaaatg acggatgcag gattccacgg
     3121 aatacctcag ctctatcggt cgaatcagca atgggttgcc cgattcatca ccggggcctc
     3181 cgtagcggaa ttgcattcga tcggaatccc ggacagtaaa atcatgccta tccatggcac
     3241 cgttgatcta acctcaagca cctcgatgag aaagcagaag cagcactact acactgccct
     3301 gcgagatgag ttgaagttac cgggggacgc ggttatcgcg ttatcagttg gtcgactgca
     3361 cccgtcgaaa gggcatgcgt ttgcgctcga ggcggtgccg gagcttgtgg ctcagtttcc
     3421 ccagctgcat tgggtagtcc tgggtgaagg agaacaacga gaggcattgg aggctcgtgc
     3481 gagcgaactc aaggtaaggg aacatgtaca tttgcttggg tttcagcggg acccattgcc
     3541 gtattatgcg gccgccgata tttatctgag gacgccggtg tttgaaagtg aaaatttgtc
     3601 cagctaccaa gccatggcaa tgggccttcc agttgttgga tttgataccg gatgtgaaac
     3661 agagctactg gggaagagtg gccatggcgt gttggttccc gcgaggaatt cggaagccct
     3721 tgcaaaagcc gtggtggaga tgttgagcct tcctgaccga ggcgcgcgaa tcggattgcg
     3781 cggcgttgaa tattgtcgtc agcatctgga catcgatcga acaatttcgg atttggtgcg
     3841 cctctaccag catctgggag caaagagggc gtgattgttg gtctgcgcgt gagtcagcag
     3901 gcgaccggct gccatgttcg gggttgaggg catgaaagtc acgtttgtca tatcagctct
     3961 ttttgcgggc ggggcggaac gtgtaatctc catcatggcc aattattggg ctcgcaaagg
     4021 gtggggcgtc accattttgt ctttcgatga tggcgagacc ccgccatttt tcgaggtcga
     4081 tccctcagtg cgccggatgt ctttgggcct tttgggagat tcggccggac tgatcgcggg
     4141 gattcggaat aacatcaatc gagtggtgag gctacgtcag gcgattctac gaagccagcc
     4201 ggacgccgtg atcagttttg tcgacaaaac caatgtgctg actctctttg cgactctggg
     4261 gttgaagctt gaagtcatgg tttcggagcg tacgaacccc tatcaatact caattggtcg
     4321 gatctggggc gcgctacgat ggtgggtgta ctctcgagct gatcgaatta tcgtgcagac
     4381 ccatgctgcc cgggcctatt tcttgccaaa atttcagcgt acagtcagcg tcattcccaa
     4441 tcccgtcctg ccgtcctcga gcaactatgc gatggtgaac catcgccctg cgggatgcat
     4501 gattctcgcc atgggtcggt tgtccaaaga gaaggggttt gatgaccttt tgtatgcatt
     4561 tcgcaaggtg aacgatcagc accccggctg cacgctcact atcatagggg agggccctga
     4621 gcgcggagaa ctggaattgt tgtgcgagag gctcggcctg tccggatgcg tgctcctacc
     4681 gggactcgtg accaccatag aagtgtttct gcggcaagca agtatgtttg tgttgagctc
     4741 ccgcttcgaa gggtttccca atgcactgtg tgaggcaatg gcctatggac ttccagtgat
     4801 tgccacggat tgtcggagcg ggccccgcga gatcattcgt gatggtgagg atggccttct
     4861 cgtcccgcct ggtgacaggg acgccttggc gactgcgatg gatagtctga tgtcagatcc
     4921 agagcggaga gcacgattag gcggtcgggc gatggacatc acgcagcggt ttggggtgga
     4981 gaaagttatg gggatgtggg agaagatcct cgaaccgatc gcatagtgaa acggggagca
     5041 tgatgacgca acaggcacag gaagtcgcgg atggccgtcg ctttgaattt gggcagaact
     5101 ggcgcaggtt tctagaggat ctcactgaag agcggattgg tcgtgcccag gaatcgctcc
     5161 aagcaatgct cgggttgaag agccttgcgg gacagcgctt tttggatatc gggtcgggta
     5221 gcggtctgtt cagtctggcc gccaaaaggc tgggggcctc cgttcgttct tttgactatg
     5281 atccgcattc tgctgcctgc accgctgaac tgaagcggcg ctatttccgg gatgatgaca
     5341 cctggatcgt tgaagagggg tctatcctgg acatggccta cgtgcagtcg ctgggaacct
     5401 acgatatcgt ctactcctgg ggtgtgctcc atcacaccgg agccatgtgg caggcactgg
     5461 acaatgcctc ccgtttagtc gctgaccagg gaacactctt tatcgccatt tataacgatc
     5521 agggcggggc cagccgccgg tggttaatgg tgaagcgtgt gtataatcgg tcccctcaat
     5581 gggggaaaat tctggtggca tggctggcat gtattcgtca gtggacgctc accgtcgtcc
     5641 gtgacacgtt cttgggcaac ccacttcgga cgtggtttag ttacaaggaa caacggggaa
     5701 tgtctccttg gcgggatgtg attgattggg ttggaggcta tccattcgag gtggccaaac
     5761 cagaggagat tttcgatttc tatcggaaaa aggggtttga tttgcagcga atgaaaacct
     5821 gcgcgggtgg tatcggctgc aatgaattcg tgtttcaccg cgtgtccaca gagcgcctgt
     5881 gaaagttcgt ttccttatcc ggtcgctgca gcggggtgga gcggagcggc agtgcgtggc
     5941 gcttgcaaaa gggttgcgcc agcttggcca cgatgtcggt gtccttgtct tctattcagg
     6001 cggggacttg tacgacgaat tattggcggc tggaattccc gttgaaagtg tcgggaaact
     6061 gcgacgctgg gagttggttg gatttttaca gagaatccgg cggtgcttac ggcgagagcg
     6121 tccggacgtg gtgtacagcc tgctgcaggt gtccaatgtg gtggcagcgg tggagaaact
     6181 cttcgttccc acgctgcgag tcacatgggg gattcgaacc gcctccatgg acctgacgca
     6241 ctatgatcgc ctcgtccgcg tctcctattg gcttgaagcg cggttgtccc gattcgcgga
     6301 tcacatcatc gtaaattccg aagctggcct tcgttatgcg aaggtggagg ggtaccgcac
     6361 ggatcgaatc tcggttattc ccaacggcat cgacaccgaa ctgtttgcgc ctgaccgatc
     6421 caagggtaag accgttcgat gctcttgggg gatcagggat gaggaggtgc tgatcgggtt
     6481 ggtcgggcga ctcgatccta ttaaagatca ccccaccttt ctgcgagcgg ctgcactggt
     6541 tgttcggcag tgcgctctgg tgaaattcgt gtgcattgga gggggagatt cggcatacaa
     6601 ggagtcgatg gtcgcgctgt gcgcagagct ggctttgtca gcccgcgtga tttgggcagg
     6661 cgaacatagt gatatgcccg cagtctataa cgcactggac gttgcctgcc tgacatcgac
     6721 cgccgagggg tgccccaaca ctcttcttga agccatggcc tgcggtgtgc cctgtgttgc
     6781 aacagatgtc ggtgattcga agcgaatcct gggggagatt ggaggcatcg ttccatctcg
     6841 caactcagag gccctggcgg gtgccataca agaattgtta cgaatggatg gcgccgaacg
     6901 ggcaaagcaa gggcagatgg gccgggatcg cgtcatccag catttcagtc ttgatcggtt
     6961 gatcggtgat acggcctccg ttcttgaggc gactgcgcga tgaccgttgt cgggatgtgc
     7021 atgacatgtg caggccgctc ttgtttcacg gtcgtcttcc gattcccgga cggcatacca
     7081 tggtgcgacg gtgacccctt ggggacggta tcagaatcgg tgatgaaaca gtccaagagt
     7141 ccctgtgagt tcggattctc tctgtcagca tgattcgaat catccatgta atctccggtc
     7201 ttgataccgg tggcgccgag gtgatgctcg tcaaggtgtt gcaaggacta gatcgcacac
     7261 ggttcacgaa tattgtcatt tcgcttacag accggggaca gttgggagaa cagatcgaag
     7321 cgtcgggcat tgcgctccac acgctgggga tgaagcgcgg gtgtcccaat ctttccgcgc
     7381 tcgtgcggtt acggcgattg ttcacgacac tgcagcccgc gattgtgcaa agttggctct
     7441 atcatgccga tctactgagt acgctggctg tcaaggtgtc gggctcgccc attctaatct
     7501 ggaacctacg ctgctcgcac atggatctca cagggtactc gcgtctcacg cgaatcgtgc
     7561 aatggatgct ggtctggctc tcccacgtgc cagttgcggt catcgccaac agcgtagcgg
     7621 ggaggcagca gcatgcccgt ttgggctatc gcccacggcg ctgggtggtg attccgaatg
     7681 gattcgatct gcagcggttt cgtcctgatc gaagtgctcg agcggcgatt cgaaccgagt
     7741 ggggcctgtc agacgatacc gttctcgttg cgtttattgc gcgggtcgat ccgatgaaag
     7801 accacgccac gtttcttgat gcggccaagc gagtggtaga tgcgaaaaag aaagcccatt
     7861 tcgtcctggt aggaaaggat acagagacgc tcgcgcccag tgtcgctaaa aggggcttga
     7921 cggatcatgt actgctctta gggcatcacg gccatatcga acggctgtta cctggtgtcg
     7981 acgtggggtg tctcagttcg ctgggcgagg ggtttccaaa cgtcctcggc gaagcgatgg
     8041 cctgtggcat accttgtatc tccacggatg ttggcgatgc tcgtgacatt attggtgaca
     8101 ccggactggt ggtgccgctc cgcgatcctg ctgcccttgc cggtgcgatg actgacctga
     8161 ttgatcgtgg gccggcgggg cgcgcgaaac tcggatatgc ggcgcgtgca cgcattgagg
     8221 cggaat
//
LOCUS       DAOQEW010000171         8218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2997_length_8218_cov_8.141125, whole genome shotgun sequence.
ACCESSION   DAOQEW010000171 DAOQEW010000000
VERSION     DAOQEW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_8218_cov_8.141125"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PLS35_15385"
     tRNA            complement(1..76)
                     /locus_tag="PLS35_15385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Ala,seq:cgc)
     gene            complement(154..825)
                     /gene="purN"
                     /locus_tag="PLS35_15390"
     CDS             complement(154..825)
                     /gene="purN"
                     /locus_tag="PLS35_15390"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNI20106.1"
                     /translation="MSAKAPRLVRLGVLASGRGSNLQAIIDAIEQHALSAEIAVVLSN
                     KQEAVALERARKHGAPAVWLDPKPFAGRPDSREAYDRAVQEVLQKHEVDLVLLAGYMK
                     IVTAVLVTAYQNRMMNIHPSLLPSFPGLDVQKKAIEHGCKIAGCTVHFVTEGVDEGPI
                     IIQAAVPIAEGDTPETLAARILEHEHRIYPRAIQLYAEGRLRVEGRRVFVAEPESPVT
                     GFHYP"
     gene            complement(815..1852)
                     /gene="purM"
                     /locus_tag="PLS35_15395"
     CDS             complement(815..1852)
                     /gene="purM"
                     /locus_tag="PLS35_15395"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNI20107.1"
                     /translation="MTTYRDAGVDIDAGDEFVERIKPHVRATFRPEVMTDLGGFGGLF
                     RFQANRYQDPVLVSGTDGVGTKLKIAFLMDKHDTVGIDLVAMCVNDIAVSGAEPLFFL
                     DYFATGKLSIATAAAVVKGISDGCRQAGCALIGGETAEMPSFYGEGEYDLAGFAVGVV
                     DRPKIIDGKQIVPGDVVIGLASSGVHSNGFSLVRKVLLERSGLTVTTAMPELGGTLGE
                     TLLTPTRIYAKQVLSLMESCPIKGIAHITGGGITDNLPRVFPARCGARIRRGSWPVLP
                     IFEAIRERGSVAVDEMYRVFNMGIGLILVVAPDHADRVMAKARELGEQAYHIGEMVAQ
                     QTEDGVVEYVG"
     assembly_gap    1902..2001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2002..3371)
                     /locus_tag="PLS35_15400"
     CDS             complement(<2002..3371)
                     /locus_tag="PLS35_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI20108.1"
                     /translation="MSASILIVDDEVSILNSLSSILEDEGYDVSVAKSGMEALKHCAI
                     SPPELMMLDIWMPEMDGLETLKRLRELVPSTQVMMMSGHGSIETAVKAIKLGAYDYIE
                     KPLSLENVTLRVKHALDQHRLEQENRNLRTKVERKFELIGQAPVMQQLKQLIETAGPT
                     NSRVLIGGENGTGKELVARAIHQHSHRATRPFVAVNCAAIPETLIESELFGHERGAFS
                     GATTMKRGQFEQADGGTLFLDEIADMSLSTQAKVLRALQEQQFTRVGGTKLIKVDVRV
                     LAASNKDLLQEIEKGTFREDLFYRLNVVPIVVPTLRDRREDIPLLVKHFLRVHAEEQG
                     LKIKQVSPEAMDVFMQYEWPGNIRELRNLIERLMIMVPGPVIEAAHASVALQVRPQMS
                     AAQSAAGAQPANTLLTRPYDSLRDARNAFEKEFIARKLREHHWNISRTAEDLKIERSH
                     LHRKIK"
     gene            complement(3420..5717)
                     /locus_tag="PLS35_15405"
     CDS             complement(3420..5717)
                     /locus_tag="PLS35_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20109.1"
                     /translation="MPESTDMSKLIPPGVLREKEPSSPASIESERRKRHVRPVWIVLI
                     LLLPCLALTFYYAQMAVPVGEESDSFLPSTGYAFVLLLVNLDLIGFVVLTLLLSRNLI
                     KAYFERRHRLVGSGFRAKLVAAFIGFSLIPTVLLALVASGLVNKAVDVWFNDQIEHVM
                     KDSYEVARMHHAGHVSLAINSARAISHEIFREELLLPEQRDLLVAAIARKRDEYATAG
                     IEVFSAKMETLTKALDPEVPVTVLDLPIGQLVLQVINGKQELTSVQEAQTGRLVRAGV
                     PIASSVRRGEIDGVVVVDAYVPESLLGKMESIGRQYAEYKQMKAMKNPIKAGAYLLVA
                     VITVMILFSATWFGFYVARGITVPIQRLAEATEAIAQGDLSVRIEAKATDEIGTLVES
                     FNRMTADLQGSKSKVEEANVSLRQSNLELDRRRAYIETVVDTIAAGVLSIDRQGIVTT
                     FNPSAERMLGLWADRFRGRSANEVFKEYQLDLFQSVYDRMLADQRDNIALEGQLDLQG
                     RFLTIGVHCSRMKDESNKDLGFVLIFEDLSELIKAQKAAAWREVAQRIAHEIKNPLTP
                     IQLSAQRLRKKFQDKAPDFDRICDESTQVIVNEVGSLKQMLDEFSKFARMPAPHMTMN
                     SLDDVVKEVVALYESAHREIICVVSLDADLPPFNFDREQIKRVLVNLCDNGIHAMNHK
                     GRLWITTRYDTKQRRAVVTVADEGTGIAPEDQDKLFVPYFSRKKTGTGLGLAIVRRIV
                     TDHDGQIHAGNHQPKGALFTFELPV"
     gene            complement(5921..6340)
                     /locus_tag="PLS35_15410"
     CDS             complement(5921..6340)
                     /locus_tag="PLS35_15410"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNI20110.1"
                     /translation="MGDEKDQGFVIRDRRGRGEEAPAAASAPTPPAPEPAAAESHHAD
                     AHGHAGHLPVNFSSFVISMGSSALMLMGEQLDPQQPAMPLNLPQAKEIIDLLSMLEEK
                     TRGNLTPDEQVVMKDMLYALRMKFVSLTSGKSSIHTP"
     gene            6476..7372
                     /gene="mazG"
                     /locus_tag="PLS35_15415"
     CDS             6476..7372
                     /gene="mazG"
                     /locus_tag="PLS35_15415"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNI20111.1"
                     /translation="MSERFDKVVAIMARLRAPGGCPWDRKQTHESLKPYLIEETYEAL
                     DTIDRRDFPKLKEELGDVLLQVLFHSQIGTEAGTFTMDDVLQELGDKLVRRHPHVFGE
                     NASGTPGLNSDQVVHRWEDIKRAERKNAGKPDSVLQDIPQALPALLRAYQTQVRAARV
                     GFDWPDSPQGLADVLGKVEEEIGELRKAVSDAGAMSQPPSEPTADMAAELGDLLFSLV
                     NLARHIKVNPEESLRQATNRFTARFQHIEHEAARSGRSVHDLTLAEMDHYWNAAKQLE
                     SADRPAAASPLASKGEASQTSL"
     gene            7369..7983
                     /locus_tag="PLS35_15420"
     CDS             7369..7983
                     /locus_tag="PLS35_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20112.1"
                     /translation="MSSEQDPYEEGKRAGLHPLLVLFGAILVMWLLINLAVPSGKQKQ
                     AAGPEISQVGTEDPDAAPVMYKVQTTLTEFNAISILVPPQSTMSQVVGLLKQFKQARL
                     NQTLAGMLPATSPGHKLGDQAVADLYIFSDPKYASPEVVGTLARGAHAPGNLYPQAIP
                     FEEAMEQVRGHYRIDLNDTSNPDTGSIGFADDSGVHSKQYRRIF"
BASE COUNT         1737 a         2538 c         2216 g         1627 t
ORIGIN      
        1 tggtggaggg caggggagtt gaacccccga cccctacgtt gcgaacgtag tgctctccca
       61 actgagctag ccccccacaa ccaggccgca ttatacacaa ccggcgccgc agtctctagc
      121 gggctacgga aaaagtctcg acgaggtctt ctatcaggga tagtggaaac cggtcaccgg
      181 agattcgggc tccgccacaa agacccgccg tccctccact cgcaaccgcc cttcggcata
      241 gagctggatg gctcgcggat aaatgcggtg ttcatgctcg agaatgcgag ccgctaacgt
      301 ctcgggcgtg tctccctctg cgatcggtac tgccgcttga atgatgatcg gtccttcgtc
      361 caccccttcg gtgacgaagt gcaccgtaca gccggcaatt ttgcagccgt gttcaatggc
      421 ttttttctgc acatctagcc ctggaaacga cgggaggagt gacggatgga tattcatcat
      481 gcgattttga tatgccgtga ccagcaccgc tgtgacaatc ttcatgtatc ccgccagcag
      541 caccagatcc acttcatgct tctgcaagac ctcttggaca gcccgatcat aggcctcacg
      601 actatccggt ctgccggcga agggtttggg atcgagccag accgccggcg cgccatgctt
      661 gcgcgctctt tccagagcga cggcctcctg cttgttgctc agcaccaccg caatctcggc
      721 cgacagcgca tgctgttcga tggcgtcgat aatcgcctgc aggttcgacc cgcgtcccga
      781 ggccaataca ccgagccgca ccagccgagg ggctttagcc gacatactcg accaccccgt
      841 cttcggtctg ctgcgcgacc atctcgccga tgtgataggc ctgctcccct aactcgcgag
      901 ccttcgccat gacccggtcg gcatggtcgg gagcgacaac caggatcaag ccgatcccca
      961 tattgaagac gcgatacatt tcgtctacgg ccactgatcc gcgttcccgg atcgcctcga
     1021 aaatcggcag caccggccag gagccacggc gaatccgtgc gccgcaacgc gccggaaaaa
     1081 cccgtggcag gttgtcggtg atgcccccgc cggtgatatg ggcaatccct ttgatcggac
     1141 aagattccat cagcgacagc acctgcttgg cgtagatgcg cgtgggcgtc agcaaggttt
     1201 ccccaagtgt cccgcccaat tccggcatgg ccgtggtgac ggtcagcccg ctccgctcga
     1261 gcaacacctt cctgacgagg gaaaagccgt tgctgtgcac accgctcgag gccaacccga
     1321 tgaccacatc acccggaacg atctgctttc catcgatgat cttcggccga tccaccacac
     1381 ccacggcaaa cccggccagg tcgtactcac cttcgccgta aaacgagggc atctcggccg
     1441 tttctccccc gatcaaggca catccggcct gtcggcagcc gtcggaaatg cctttcacca
     1501 ctgccgccgc cgtcgcgatc gacagtttac cggtggcaaa atagtcgagg aagaacagcg
     1561 gctccgcgcc actcacggca atatcgttca cacacatggc caccaggtcg atgccgaccg
     1621 tgtcatgttt atccatcaga aaggcgatct tgagtttcgt cccgacgccg tcggtccccg
     1681 acacgagcac cgggtcctgg taccgattgg cctggaaccg gaagaggccg ccgaagccgc
     1741 ccagatccgt catcacttca gggcgaaacg tggcccgcac atgcggcttg atccgctcga
     1801 cgaattcatc gccggcatcg atatcgactc cggcatcacg ataagtagtc attggggtcg
     1861 catcttaacg agccaactga tagcgggtca acaatagtct cnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn naacttaatc ttccgatgta aatggctgcg ttcgatcttg
     2041 aggtcttccg ccgtgcggga aatattccaa tgatgctcgc gcagtttgcg ggcaatgaat
     2101 tccttttcaa aggcattccg cgcatcgcga agcgaatcgt acggcctggt cagcaaggta
     2161 ttggccggtt gggcgccggc ggccgattgg gcggctgaca tttggggacg cacttgaagc
     2221 gcgacggacg catgtgccgc ctcgatgacc ggtccgggca ccatgatcat cagccgctcg
     2281 atcagattcc gcaattcacg gatattcccc ggccattcat actgcatgaa cacgtccatg
     2341 gcctccggtg acacctgctt gatcttcaac ccctgctctt ccgcatgcac gcgcagaaaa
     2401 tgtttcacca gcaagggaat gtcctcgcga cgatcacgca aagtgggcac tacgatcggc
     2461 accacgttca ggcggtagaa caagtcttca cggaacgttc ccttctcgat ttcctgcagc
     2521 aaatccttat tcgatgcagc cagcacccgc acgtcgacct tgatcagctt ggtcccgcca
     2581 acccgcgtaa actgttgctc ctggagagcc cgcagtactt tggcttgcgt gctcaagctc
     2641 atgtctgcga tttcatccaa aaataacgtc ccgccgtcgg cctgttcgaa ctggccgcgc
     2701 ttcatggtgg tggctccgct gaaggcacct cgctcgtggc cgaacaactc gctttcaatc
     2761 agggtctcgg gaatggctgc acagttgacc gccacaaacg ggcgagtcgc cctgtggctg
     2821 tgttgatgga tggctctcgc caccaattcc ttaccggtgc cgttttcgcc gccgatcagc
     2881 actcgactat tggtcgggcc ggccgtttcg atcagttgct tcagttgctg catgaccggc
     2941 gcctggccga tgagctcgaa cttccgctcg actttggtgc ggaggttgcg attctcctgc
     3001 tcgagccggt gttgatccaa ggcatgtttc accctgaggg tcacattttc gagggacagc
     3061 ggcttttcga tatagtcgta cgcacccagt ttgatcgcct tgaccgccgt ctcgatcgaa
     3121 ccgtgcccgg acatcatcat gacttgagtg ctgggcacca gttcgcgcag tcgcttgagg
     3181 gtctccagcc cgtccatctc gggcatccag atatccagca tcatcagttc aggcggactg
     3241 atcgcgcaat gtttcagggc ctccatcccg cttttggcca cactcacgtc atatccttcg
     3301 tcctcgagaa tactgctcaa ggaattcagg atggagactt cgtcatcaac aatgagaatc
     3361 gaagcagaca tgcatccccc acatacgatc cgccaacacc cactcgaccg tccgttccct
     3421 tacaccggca attcgaaggt aaataaggcc cccttcggct gatgattccc cgcatgaatc
     3481 tgaccatcgt ggtcggtcac gatccgcctg acgatggcca accccaagcc cgtcccggtc
     3541 ttctttctgg aaaaatacgg cacaaagagt ttgtcctgat cctccggcgc gataccggtg
     3601 ccttcatccg ccaccgtgac caccgcgcgg cgttgcttcg tatcgtaccg ggtcgtgatc
     3661 cacaagcggc ccttgtggtt catggcatgg atgccgttat cgcacagatt caccaggaca
     3721 cgcttgatct gttcgcgatc gaaattgaaa ggcggcagat cggcatcgag cgacaccaca
     3781 cagatgattt ctcgatgggc actttcgtag agcgcgacga cttccttcac gacatcgtcg
     3841 agcgaattca tcgtcatatg cggcgccggc atgcgcgcga atttcgaaaa ctcatccagc
     3901 atctgcttga ggctaccgac ctcgttgacg atcacctgcg tggattcgtc gcagatacgg
     3961 tcgaaatccg gtgccttgtc ctgaaatttc ttccgcaacc gctgggccga gagttgaatc
     4021 ggcgtgaggg gattcttgat ctcgtgcgcg atccgttgcg ccacttcccg ccaggccgcc
     4081 gccttctgcg ccttgatcaa ttccgacagg tcttcaaaaa tcagcacgaa gccgagatct
     4141 ttattcgact cgtccttcat gcgtgagcaa tgcacaccga tggtgagaaa gcgcccctgc
     4201 aggtccagct gcccttccag cgcgatgtta tcccgctggt cggccagcat gcgatcgtag
     4261 acggattgaa acaggtcgag ttgatactct ttgaacacct cgttggcgga gcgcccgcga
     4321 aaccggtcgg cccacaaccc cagcatgcgt tcggccgatg gattaaaggt cgtgacaatc
     4381 ccctgccgat cgatcgacaa cacccccgcg gcaatcgtat cgaccaccgt ttcgatatag
     4441 gcgcgccgcc ggtccagttc gaggttggat tggcgcaagg agacgttcgc ctcttcaacc
     4501 ttgcttttgc tgccctgcag atcggcggtc atccggttga aggattccac caacgtcccg
     4561 atttcatccg tcgccttcgc ttcgatccgg accgataaat ccccctgggc aatggcctcg
     4621 gtcgcctcgg ccaaccgctg aatcggcacg gtgataccgc gcgcgacata aaacccgaac
     4681 caggtggcgc tgaacaaaat catcaccgtg atcaccgcca ctaacagata ggcgcccgcc
     4741 ttgataggat tcttcatcgc cttcatctgt ttgtactcgg catattgccg gccgatgctc
     4801 tccattttgc cgagcaacga ttccggcaca taggcgtcca ccacgaccac cccgtcgatt
     4861 tccccgcgcc gcacgctgga agcgatcggc acgccggcac gcacgagccg gccggtctgc
     4921 gcttcctgca cagaggtcaa ctcctgtttg ccattgatca cctgcaacac cagctggccg
     4981 atcggcagat cgagtacggt caccggcacc tcaggatcca gggccttcgt gagcgtttcc
     5041 attttcgcgg aaaacacctc aatgccggcc gtggcgtatt catcgcgttt ccgggctatc
     5101 gctgcgacta acagatcccg ctgctccggc aacagcaatt cctcgcgaaa gatctcatgg
     5161 ctgatggcgc gagcgctgtt gatcgccaac gacacatgtc cggcatggtg catgcgcgcc
     5221 acctcgtagg agtctttcat cacatgctcg atctgatcgt taaaccagac atcgacggct
     5281 ttgttgacca acccgctggc gaccaaggcc aacaacacgg tcggaatgag ggagaatccg
     5341 atgaaggccg cgaccaactt cgcccgaaac ccggagccca ccaggcgatg ccggcgttcg
     5401 aaataggctt tgatgaggtt gcgcgacaga agcagcgtga ggacgacgaa cccgatcagg
     5461 tcgagattga ccagcagcag gacaaaggca tagccggtgc tgggaagaaa ggaatcggat
     5521 tcctctccga ccggaacggc catttgtgca tagtagaagg tcagcgccag gcagggcagc
     5581 agcaggatca ggacgatcca gacgggccgg acatggcgct tgcgccgctc gctctcgatc
     5641 gaggcgggcg acgacggctc tttttcacgc agcacacccg gggggatgag cttgctcatg
     5701 tccgtcgatt caggcatcgg catgactccg aatccatctc ggaccctttg ggttctgcca
     5761 cactataacg aattcctgag gaaagactca aaagctgtgc cgaagccatc gcgctggtcg
     5821 gaaacatgaa caagacactg agagaggttg agaagccggt cgcaaccagg aggaagtcaa
     5881 acccggtgga atagcggaac gcgaagaggg cgatcacagg ttacggggta tgaatcgatg
     5941 atttcccgga agtcaggctg acgaacttca tcctcaaggc atacagcatg tccttcatca
     6001 cgacctgctc gtccggcgtg agattgcccc gtgttttctc ttccagcatg gagaggagat
     6061 cgatgatttc cttcgcttgc ggaagattca gcggcatagc cggttgctga ggatccaatt
     6121 gctcccccat gagcatcaaa gccgagctgc ccatggaaat cacgaaggag gagaaattga
     6181 ccggcagatg cccggcatgg ccatgcgcgt cagcatgatg ggactcggct gctgccggtt
     6241 cgggagcagg aggcgtggga gccgaggctg cagcaggcgc ctcttcaccg cggcctcgac
     6301 gatcgcggat gacgaatccc tgatccttct catcacccat gtgcgcaacc tcccgtcagt
     6361 ccatcataaa atgcaggacg ataccctacc acaggccttg agggtgcgca aggcggcggg
     6421 ccccgttgaa ggtgaagata aactcagtta gagtggcgcc acgaggggaa accgcatgtc
     6481 cgaacgtttc gataaagtgg tggcgatcat ggcccggctc cgggcacccg gcggctgccc
     6541 atgggatcgc aagcaaacgc atgaatccct caagccctac ctgattgaag aaacgtacga
     6601 agcgcttgat accatcgacc ggcgggattt ccctaaactc aaggaagaac tcggcgatgt
     6661 gttgctccag gtgctcttcc acagccaaat cggaacggaa gccggcacgt tcaccatgga
     6721 tgacgtgctc caggagttgg gcgacaaact cgtgcgccgc catccgcatg tcttcgggga
     6781 gaacgcgtcc ggcacgccgg gtctgaattc cgaccaggtc gtgcatcgat gggaagacat
     6841 caagcgagcc gagcgtaaga atgccggaaa acccgattcg gtcttgcaag atatccctca
     6901 agccctcccg gcgttacttc gcgcctatca aacccaggtt cgcgccgcac gggtcggttt
     6961 cgactggccg gacagcccgc aagggctcgc tgatgtcctc ggcaaggtcg aggaggaaat
     7021 aggggaactt cgcaaagccg tgagtgacgc gggggctatg tcccagcctc catcggaacc
     7081 taccgcggac atggcggcgg aattgggcga cctgctcttc tccttggtca acctggcgcg
     7141 tcacatcaag gtgaatccgg aagaatcgct gcgtcaggcc accaaccggt tcacagctcg
     7201 cttccagcac atcgaacatg aagcggcgcg cagcgggcgc agcgtgcacg atctcacgct
     7261 ggctgaaatg gatcactact ggaacgccgc aaaacaattg gaatcggccg atcgtcctgc
     7321 ggcagcctcc ccgctcgctt ctaaaggaga ggcgtcgcag acttccttat gagcagtgaa
     7381 caggatccat acgaagaggg caagcgagcg ggactgcatc cgctgctcgt gctcttcgga
     7441 gccattctgg tcatgtggct gctgatcaat cttgccgttc ccagcggaaa acaaaagcag
     7501 gcggccggac cggaaatctc tcaagtcggc acggaagatc cggatgccgc gccggtcatg
     7561 tacaaagtgc agacaacgct gacggaattt aatgccatta gcatcctcgt cccaccccag
     7621 tcgaccatga gccaggtcgt cggtctgttg aaacaattca aacaggccag actcaatcag
     7681 acactggccg gtatgctccc ggcaacaagc cccggtcaca aactgggtga tcaggcagtc
     7741 gccgacctgt acatcttttc ggaccctaaa tatgcctcgc cggaagtcgt cggcacgctg
     7801 gcgcgcgggg cccatgctcc gggcaacctc tatccgcagg ctatcccgtt tgaagaagcc
     7861 atggaacagg tacgggggca ttaccgcatc gacctgaacg acacgagtaa tccagacacc
     7921 gggtccatcg gatttgccga tgattccggg gtgcacagca agcaatatcg ccggattttt
     7981 tgagcagatc tctcgaaatc ccacgcctga cgcgccttcg ctatccccaa gctaaaatcg
     8041 gtcagcaaat atccccctag gggttttcct cttctacagc atctagtggc ctgcgccagg
     8101 acgaccccca atgcctaaaa cttgtgcagg ccgctgaagg accagcccac gaagggcctc
     8161 gagccgccat aggcccccta tacacatcct tatccacaga ttctgtgtgt gagataac
//
LOCUS       DAOQEW010000172         8217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_2998_length_8217_cov_7.641632, whole genome shotgun sequence.
ACCESSION   DAOQEW010000172 DAOQEW010000000
VERSION     DAOQEW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2998_length_8217_cov_7.641632"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /gene="pstB"
                     /locus_tag="PLS35_15425"
     CDS             <1..660
                     /gene="pstB"
                     /locus_tag="PLS35_15425"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNI20113.1"
                     /translation="TAFIGPSGCGKSTLLRCLNRMNDLVEGARPVGRVELDGMDIYDP
                     AIDVTDLRKRVGMVFQKSNPFPKSIFDNVAYGPRLHGIKDKRSLDERVQHSLQGAGLW
                     DEVKDRLQHSAVGLSGGQQQRLCIARALAVQPEVILMDEPCSALDPIATGKIEELIYS
                     LKETYTVVIVTHNMQQAARVSDQCGFFLMGELVEFGDTKTIFTTPRDKRTEDYITGRF
                     G"
     gene            682..1350
                     /gene="phoU"
                     /locus_tag="PLS35_15430"
     CDS             682..1350
                     /gene="phoU"
                     /locus_tag="PLS35_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNI20114.1"
                     /translation="MQRHFDQDLAHLKQQLLRMGGLVESQIQQALQALVDRDSDLAVD
                     VIKQDHDVNGLDVEIDELCVQLLALQQPTARDLRFVTTGMKISSELERMGDLADNIAQ
                     RALELNSEPQLKPYIDIPRMANWTMRMVKECLDAFVNSDPVLARKVCTDDDFVDEVNE
                     QLFRELLSFMLEDTRTITRAIRLTFVAKSLERIADHATNIAELVVYMVEGKNIRHVAP
                     TASF"
     gene            1351..2904
                     /locus_tag="PLS35_15435"
     CDS             1351..2904
                     /locus_tag="PLS35_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNI20115.1"
                     /translation="MPKLAVLDIGTNSIHMVLAEVQPDYSYKILDRFKDMTRLGDGVF
                     TSRRLSDQAMMRGLEVIRTLVNLARNKGYEQIEGVATSAVREARNGGEFLDHVMQQTG
                     LTVRVITGAEEARLIFLGVQNSVALPESPTLVIDIGGGSVEVIVGNREAIFQARSLKL
                     GAIRLKDLYLSKTPPSKAMLRDLEAAVTAQLKNALGSYKTKRVEQIIATSGMAGNLAE
                     VIHLQRTGRPLPQLNLARVSAKDIAAVEKRLAGASLKTRLAMPGLDPKRVDTLLPAAI
                     VFRILLELLQKTELTLCDKAIREGIIYDFIQRHHDRIQAERDIPDVRKRNILALAHRC
                     HVSETHALHVAGLALRLFDQTKALHGLGQREREWLESAAILHDIGYMINSRQHHKHAY
                     YLIKNSDLSGFTAEEIDLIANIARYHRRSVPTRKHDEFTVLPEGSQRVINVLSALLRI
                     ADGLDRSQFSVVQNIDVKLGKTVVITAQVSGDAELEIWAARGRSDLFEKVFKRPVQFI
                     TTTRDDDAT"
     gene            complement(3030..3527)
                     /locus_tag="PLS35_15440"
     CDS             complement(3030..3527)
                     /locus_tag="PLS35_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNI20116.1"
                     /translation="MDCLLFRHGIAYDREDWRGNEHNRPLTEKGLVRTRQSSKGLLTL
                     GCIPTLILSSPLTRAQQTAKIARSLIPATPPLRICQELNPEVPPRALIALLNTLSSDA
                     VVLCVGHEPHLSATAGILLTGKLCKGLSLKKAGACLLHLEGSVRPAKGRLEWWLTASQ
                     LRALA"
     gene            3630..4484
                     /locus_tag="PLS35_15445"
     CDS             3630..4484
                     /locus_tag="PLS35_15445"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNI20117.1"
                     /translation="MGDMLHSIHTGPQSPTQGLLTMAASSHATVLHLLRQAIAGREHV
                     DTIHAIRTHCRRLQALLELYGEAKRARAVAACVRRLSKLRALQVFRQYVIRCHAGRKD
                     VAVVDDRLTKQIRKLNHTEAYRRIEQVVSKQALPAILSPPQSWNRRLDELRQEHECAL
                     KRLIAAASDKPRRKRLHALRLALKTIRYQTEWLPGRTAAKEEMLSQLKRVQSLLGKYE
                     ELADFHRWGKKLSLPVRKRMKKDWKRARKRARHVPGTMSWLLDALASGHVWSGENLHV
                     ASVNRRSA"
     gene            complement(4461..5183)
                     /locus_tag="PLS35_15450"
     CDS             complement(4461..5183)
                     /locus_tag="PLS35_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20118.1"
                     /translation="MALEIIEIVEDDQSQAKLLDQILRQASYRTNVAFDGPAGMQDVW
                     RIKPSLIVADDNLPGLTGREMCKRLRQDPSTTHIPIIVMSGFSSEERRAEALDAGADD
                     FIVKPYSGMELLARVRALLRRCRQSQAQDEELPEDLALTENLYVAVYRGKSLTLSAHE
                     WKALRRLASTVGNVVPREELKSLLWGDDSLMHDGELDRCMQQLNRKLSQDAPLVESIK
                     IVPGGFRLSTPASDDSGGPAVH"
     gene            complement(5273..6697)
                     /locus_tag="PLS35_15455"
     CDS             complement(5273..6697)
                     /locus_tag="PLS35_15455"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI20119.1"
                     /translation="MYERFYHLTVKPFTLVPNPDFLYRGETHRLALTTLEYGILNHAG
                     FVVLTGEPGMGKTTLIQQILAEHRNHFTTGLITNTHEGMETLLPWILLAFGQNDKNLD
                     KLEGFHAFQQFLGKEAAAKRRVLLIVDEAQNLGANLLEELRLLSNLNKDKAPILQIIL
                     SGQPDLRQLLQRKDLTQFAQRVMVDYSLEPFKEEDSIGYIAHRLRVAGREEPLFTHEA
                     ARTIHRLTGGIPRLINQLCDTALTYGFAEEAPFLSARIVAQAAKDRTKGGILPLSNTE
                     SLTAVTPDQQAAEQTELAALSARAASTSNEVPRRDDAVPTSSGVATARPDTNEQYERG
                     LSLKKAGSYKEALQAFSLAAQDAAFTFKAMAQVGICLKSLAQPEEAVTAFRKSLQAKR
                     SASPDTIQVRYLLARTLESLGRTDDALEQYRWIGREEPSFKDVAERLERLNGRPMRAT
                     RNEAASSDGSSWVTQLQRILGASK"
     gene            complement(6724..7560)
                     /locus_tag="PLS35_15460"
     CDS             complement(6724..7560)
                     /locus_tag="PLS35_15460"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNI20120.1"
                     /translation="MEPLHSAMERFKREEQRRPTPVVLKAPPPRIGKDAVQDIVYTHT
                     RTVDIPDDVLRERRVLAGFERGPFLDAYKILRTQVMHRLRENGWNVLGITSPSKQEGK
                     TLTAVNLAISLAMDTTQTVLLVDADLTNPSAHLAFGIEECGGLVDYLLDGIPVEHLLI
                     HPGIGRFVLMPAGRSITNSTEALTSPKMASLVEEFKHRYPSRIVLFDLPPLLQTADVL
                     AFSPYTDALLLVVEDGKTTREEVERALQLVKGSTPVLGTVLNKAGKGHMTPAAMKRML
                     LT"
     gene            complement(7579..>8217)
                     /locus_tag="PLS35_15465"
     CDS             complement(7579..>8217)
                     /locus_tag="PLS35_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20121.1"
                     /translation="AIKPENPAYINTQTQLASATSMLEALKAQRAKLRKRAEDYARRL
                     EKTHHFEPEYLDLARERDNSARKYEELRSKLLEAQVSEGLETQRKGERFSLIDPPDLP
                     EKPEKPNRPLILLLSFVLALAGGAGSGVLLENVDRSIRGPESLAHITQTYPLAVIPYI
                     PNNADLRQLIAKRRTIRLAGIGIAAACILLAHFFWLPLDVVWFAALRKFGLD"
BASE COUNT         1754 a         2218 c         2420 g         1825 t
ORIGIN      
        1 acggccttca tcggcccttc cggttgtgga aagtccacgt tgctccggtg cttgaatcgc
       61 atgaacgatc tggtggaggg agcccgtccg gtaggccgtg tggaattgga cggcatggat
      121 atctacgatc cggccatcga tgtcaccgat ttgcggaagc gcgtggggat ggtgtttcaa
      181 aaatccaatc cctttcccaa atccatcttc gacaacgtgg cctacggtcc gcgtctgcat
      241 gggatcaaag ataaacggtc gctcgacgaa cgggtccaac acagtttgca aggcgcaggc
      301 ctttgggatg aggtgaagga tcgccttcag cacagtgccg tgggactttc aggcggccag
      361 cagcaacggc tgtgtatcgc ccgtgccctg gcggtgcaac cggaagtgat cctcatggat
      421 gagccctgct ccgcgctgga tcctatcgcg acagggaaaa tcgaagagct catctatagc
      481 ttgaaggaga cctacaccgt cgtcatcgtg acgcataata tgcaacaggc ggcacgggtc
      541 tctgaccagt gcggattttt tctgatgggc gagttagtcg agttcggtga caccaaaacc
      601 attttcacca cgcctcgtga caaacgaacc gaagactaca tcacgggacg attcggataa
      661 atcgcgagag gagcctgaac tatgcaacga cacttcgatc aagacctcgc gcatctcaaa
      721 cagcagctgc tccgtatggg cggcctggtc gagtcgcaaa tccagcaggc cttgcaggcc
      781 ctggtggacc gagattccga tctcgccgtc gatgtcatca agcaggatca cgacgtgaac
      841 ggattggatg tcgagatcga cgaactctgc gtacagttgt tagcccttca acagcccacg
      901 gcccgtgacc tcaggttcgt caccaccggc atgaagattt cttccgagct ggaacggatg
      961 ggtgacctgg ccgacaatat cgcgcaacgg gcgttggagc tgaacagcga accgcagctc
     1021 aagccataca tcgacattcc gcgcatggcg aattggacca tgcgcatggt taaggagtgc
     1081 ctggatgcct tcgtgaattc cgatccggtc ttggcgagga aagtctgcac ggacgacgat
     1141 tttgtcgatg aggtgaacga acagttgttt cgagagttgc tctcctttat gctggaggac
     1201 acgcgtacca ttacacgcgc gatccgtctg acgtttgtgg caaaatcgct cgagcgaatt
     1261 gccgatcatg ccactaacat cgcggaactt gtcgtctata tggttgaagg caagaacatc
     1321 cggcacgtcg ctcctaccgc gagtttctag atgcccaaac tggccgtcct cgacatcggc
     1381 accaattcca tccatatggt gttggccgaa gttcagccgg attactccta taaaatcctc
     1441 gatcgtttca aagacatgac gcgtctgggc gacggggtgt tcacctcccg gcgtctttcc
     1501 gaccaggcca tgatgcgcgg attggaagtg atccgcacac tcgtgaatct tgcccgcaat
     1561 aaagggtacg aacagatcga aggtgtagcc accagcgccg tgcgggaggc gaggaacggc
     1621 ggcgaattcc tagatcatgt catgcagcaa accggcctga ctgtgcgagt gattaccggc
     1681 gcggaagaag cccggttgat tttcctcggc gtgcagaaca gcgtggccct gcccgagtcg
     1741 cccacgctgg tgatcgatat cggcgggggg tcggtggagg tcatcgtcgg gaatcgcgag
     1801 gccatttttc aggcccggag cctgaagctg ggcgcaattc gcttaaagga tctctacctc
     1861 tctaaaacgc ctccatcaaa agcgatgctt cgggatctgg aagcggcggt gaccgctcaa
     1921 ctcaagaatg ccttgggctc gtacaaaacc aaacgggtgg agcagattat cgcgacgtcc
     1981 ggcatggcgg gcaacctggc tgaggtgatt catctgcaac gcaccggccg gccgcttccg
     2041 cagctcaatc tggccagggt atccgccaag gacatcgccg ccgttgaaaa acgacttgcc
     2101 ggcgcttcgt tgaaaacgcg tctggccatg ccggggctgg atcccaaacg tgtggacacc
     2161 ctgcttccgg ccgccatagt gtttaggatc ttgctggagc tgttgcagaa aacggagctc
     2221 accctctgcg acaaagcgat ccgcgaaggg attatttacg atttcattca gcggcaccac
     2281 gaccgtatcc aagcggagcg agacattccc gacgtgcgta aacgcaacat cctggccctc
     2341 gcccatcgat gccatgtctc cgaaacgcat gcgttgcatg tggcgggatt ggccttacgt
     2401 ctatttgatc agacgaaggc gttgcatgga ctcggacagc gggaacgcga gtggttggaa
     2461 agtgcggcga tcctgcatga tatcggctat atgatcaatt cgcgacagca tcacaagcat
     2521 gcctactacc tgatcaagaa tagcgatctc tcggggttta ccgctgagga aatcgatttg
     2581 attgcgaata tcgcccgcta tcatcgccgt tccgttccta ccaggaagca tgacgaattt
     2641 acggtattgc cggagggctc ccagcgagtc atcaatgtct tgtcggcatt gctgcgcatt
     2701 gccgatggct tagaccgaag ccagttttcg gtggtgcaga atatcgacgt caaactcggc
     2761 aagaccgtgg tgattactgc gcaggtgtcg ggtgatgcgg agttagagat ttgggcggcg
     2821 cgaggccgga gcgatctgtt tgagaaagtc ttcaagcggc cggtgcagtt cataacgacc
     2881 acccgagacg acgatgccac ctgaatcggg atccacggtc aactcctaca ggcactcctt
     2941 actgcacgaa cttactttcg aagccgcgtg tattctaagc gagtgcgctc agcagtgaat
     3001 catcgaatgg cgacgcttgc gcgagcacct tacgccagtg cgcgcaattg tgacgcggtg
     3061 agccaccact ccaggcggcc ttttgcggga cgcacggacc cttcaagatg aagtaggcac
     3121 gcgcctgctt ttttgagcga aagccctttg cacagctttc ccgtgaggag gattccagcc
     3181 gtcgcgctca ggtgtggttc gtgccccacg cagaggacca cggcgtcgga cgagagtgtg
     3241 ttgagcagtg caatcaatgc acgcggtggg acttctggat tgagttcctg gcagatccgc
     3301 agcggcggcg ttgcaggaat caacgatctc gcaatcttgg ccgtttgctg ggcgcgtgtc
     3361 agggggcttg aaaggataag cgtcgggata cagcctagag tcagcaatcc tttacttgat
     3421 tgccgcgtcc gaaccaggcc tttttccgtg agcggccggt tgtgctcgtt cccccgccag
     3481 tcctcacgat cgtaggcaat gccgtgtcga aataagagac aatccatcaa cgtactccct
     3541 tgggatggac gtaaggtggt cgggccggcc ataattcatg caatgggtga tcttctgtga
     3601 gcccatcgca caggaattga ttattcgtca tgggtgacat gctccattct attcatacag
     3661 gaccgcagtc accgactcaa gggttgctca cgatggcggc ttccagtcac gccaccgttc
     3721 tccatttgtt gcgccaagcg attgcgggaa gagaacatgt ggacacgata cacgccatcc
     3781 gtacgcattg ccgaagactt caagctttgc tggaactcta cggcgaggcg aaacgtgctc
     3841 gtgcagtggc agcttgtgtg aggcggttga gcaaactgcg agcgctccag gtgttcaggc
     3901 agtacgtgat caggtgtcac gccgggcgaa aagatgtggc cgtggtggat gaccggctga
     3961 cgaaacagat caggaagttg aatcacacgg aggcctatcg caggatcgaa caggttgtct
     4021 cgaagcaggc gcttcccgcg atcctctccc ctcctcagtc ctggaatcgc cggcttgatg
     4081 aactgcgtca ggagcatgaa tgcgcgctca aacggctcat cgcggctgca tcggacaagc
     4141 ctcggcggaa acgcctccac gcgttgcgtc tcgccttgaa gacgatccgg tatcagactg
     4201 aatggttgcc cggtcgaaca gcagcgaaag aagagatgct ttcccaactc aagcgggttc
     4261 aatctttatt gggaaaatat gaggagctgg cagattttca tcgttggggt aagaagctga
     4321 gtcttccggt gcggaagcgc atgaagaagg actggaagcg agcgagaaaa cgagcccggc
     4381 atgtccccgg aacgatgtcg tggttactgg atgcgttggc atccgggcat gtgtggagcg
     4441 gcgaaaatct tcacgtggca tcagtgaacc gcaggtccgc ctgagtcgtc cgaggctggt
     4501 gtcgagaggc gaaaacctcc cggcacgatt ttgatggact ccaccaatgg ggcatcttgc
     4561 gacagctttc ggttgagctg ttgcatgcat cgatcgagtt ctccgtcgtg catgaggctg
     4621 tcgtctcccc agagcagaga tttgagttcc tcgcgcggaa caacgttccc gacggtactc
     4681 gccaatcgac gcagcgcttt ccactcgtgc gcggataacg tcaggctctt tcctcgatag
     4741 accgcaacat acaaattttc agtcaaggcg agatcttcag ggagttcttc atcctgcgcc
     4801 tggctttgcc ggcaacgacg caacagtgcg cgtacgcgag cgagcagttc catcccggaa
     4861 tagggcttga cgatgaagtc gtcggcccct gcgtcgaggg cttctgcccg gcgctcttcg
     4921 gacgaaaacc cggacatgac aatgatggga atgtgtgtgg tggaagggtc ctgccgtaag
     4981 cgcttgcaca tctcccggcc ggtcaatccg ggaaggttgt catcggccac gattaaggag
     5041 ggtttgatcc gccagacatc ctgcataccg gccggcccat cgaatgccac gttggtgcgg
     5101 tatgacgcct gccggaggat ttgatccaat aatttagcct gactctggtc atcttcgaca
     5161 atttcaatga tttctaaagc catgcggaca ctcccctgca cttatcatcg gttggtaccg
     5221 aatgaaactt gaacgcggtg gtagcaggca gaaatgtcag gagtgttgct ggctacttgg
     5281 aggcgccgag aatacgctgc agttgggtca cccaggagga tccgtcgctg cttgccgctt
     5341 cattgcgagt ggctcgcatg gggcggccgt tgagccgctc gagccgttcc gccacgtcct
     5401 tgaagctggg ctcttctcgg ccgatccagc ggtattgctc aagcgcatca tctgtgcgcc
     5461 ctaacgactc aagggtgcgc gccaacagat accgcacctg gatagtatca ggggatgcag
     5521 aacgctttgc ctgtaatgac tttcgaaatg ccgtaacggc ctcttccggt tgcgcgaggg
     5581 atttcaagca aatcccaacc tgcgccattg ctttaaaggt gaaggcggca tcctgggcgg
     5641 cgagagaaaa cgcttgtaag gcttccttat acgatccggc tttcttgagc gacagtcccc
     5701 gctcgtactg ttcattggtg tctggtcgtg ctgtagccac acctgaagaa gtcgggactg
     5761 catcatcacg gcgcggaact tcattggacg tggatgcggc tcgagctgac agtgccgcca
     5821 gttcggtttg ttcggcagcc tgttgatcag gcgtcactgc cgtgagggat tcagtgttcg
     5881 acagcggaag aatgccccct ttggtccggt ctttcgcggc ttgcgccacg atccgcgccg
     5941 acagaaatgg cgcttcctcg gcgaagccat acgtcagggc cgtatcgcac agttggttga
     6001 tcaagcgagg aatgccgccg gtcagccgat gaatcgtccg cgcggcctca tgtgtgaaga
     6061 gcggctcttc tcgccccgcg actcgcaaac gatgggcaat gtatccgatg ctatcttctt
     6121 ctttgaacgg ttcaagactg taatccacca tcacacgttg cgcgaattgg gtcagatctt
     6181 tccgttgcag cagctgtcga aggtccggtt gtcctgacaa aatgatctgg aggatgggcg
     6241 ccttatcctt gttgagattg gagagcagcc gcaactcttc cagtagattt gctccgagat
     6301 tctgcgcctc atcgacgatc aacaagaccc ggcgtttagc cgccgcttct ttgccgagga
     6361 attgctggaa cgcatgaaac ccttccagct tgtccaggtt tttatcgttc tggccgaagg
     6421 cgagtaaaat ccacggcaag agagtttcca tgccctcatg cgtattggtg atgagccccg
     6481 tggtgaaatg gttccgatgt tcggccagga tttgctgaat aagtgtggtc ttccccatcc
     6541 cgggctctcc ggtgagcacc acgaaccccg cgtgattgag gatgccgtat tcgagggtgg
     6601 tgagcgcgag gcgatgggtc tccccgcgat agagaaaatc gggattcggg acgagagtaa
     6661 agggcttgac agtcaggtga tagaagcgtt cgtacatggg tcttctttac gagagtaagg
     6721 tgtttaggtc aataacattc gcttcattgc cgccggggtc atgtggcctt tgccggcttt
     6781 gttaagcacg gtgcccaata ccggtgtgct gcctttgact aattgcaggg ctcgctcgac
     6841 ctcttcacgg gtggtcttcc cgtcctcgac gacaaggagc aaggcgtcgg tgtagggcga
     6901 aaaggccaac acatcggcgg tctgcagcag cggtggcaaa tcaaacagta cgatgcggga
     6961 tggataccgg tgcttgaact cctcgacgag cgaggccatc ttgggcgagg tcaatgcttc
     7021 cgttgaattc gtaatagatc ggccggccgg cataagcaca aaccgtccga ttccgggatg
     7081 aatcaacagg tgctcaacgg gtattccatc cagcaaataa tcgaccagcc ctccacactc
     7141 ctcaattcca aatgccagat gagcgctggg attggtcagg tcggcatcta ccagcaggac
     7201 cgtttgtgtc gtatccatgg cgaggctgat ggccagattc accgcggtca gcgttttgcc
     7261 ttcttgtttg ctggggctgg tgatgcccag cacgttccaa ccattttcgc gcagtcggtg
     7321 catgacttgg gtgcggagaa ttttgtacgc atcaagaaag gggccgcgct cgaaaccggc
     7381 aaggactcgt cgttcacgca ggacgtcgtc cgggatgtcg acggtgcggg tgtgcgtata
     7441 cacaatatcc tgcacagcat cttttccgat tcgagggggc ggtgctttga gaacgaccgg
     7501 cgtggggcgt cgctgttcct cccgcttgaa tcgttccatt gcactgtgta gcggttccat
     7561 gtgaaatctc ctactcggtt aatcgagacc gaatttacgt agcgcggcga accacaccac
     7621 atcgagcggt agccagaaga aatgtgccaa taaaatgcag gcagccgcga tgccgatgcc
     7681 ggcaaggcgg atggttcgac gcttggcaat cagttggcga aggtccgcgt tattgggaat
     7741 gtagggaatc acggcaagtg gataggtctg ggtgatatga gccagtgatt ccgggccgcg
     7801 gattgaacgg tcgacgtttt ccagtaagac cccgctgccc gctcctcctg ccaaagccag
     7861 cacaaaactc aggagcagga taagtggacg attcggcttt tccggttttt cgggaaggtc
     7921 cggcggatca atcagggaaa accgttcacc tttgcgctgt gtctcgagcc cttcggaaac
     7981 ttgtgcctca agaagtttgg aacgcaattc ttcatatttc cgggcggagt tgtcccgctc
     8041 ccgcgcgagg tctaagtact ccggttcgaa atggtgagtt ttttcgagac gccgggcata
     8101 gtcttccgca cgtttccgca atttggctcg ttgcgctttc agcgcttcca acatcgaagt
     8161 ggccgaagct aattgcgttt gggtgttgat gtacgcgggg ttctccggtt tgatggc
//
LOCUS       DAOQEW010000173         8156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3036_length_8156_cov_8.203061, whole genome shotgun sequence.
ACCESSION   DAOQEW010000173 DAOQEW010000000
VERSION     DAOQEW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3036_length_8156_cov_8.203061"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLS35_15470"
     CDS             complement(<1..378)
                     /locus_tag="PLS35_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20122.1"
                     /translation="MAQRFQSGAFVQQCFAVHPLCLSLKRIDDDGRLVIACTSCRMMH
                     LMTPDQVVGRVAAVPLGGEVESPKAVPTPRESLEDCLRAHPVSVTIRELDVIRDQVGL
                     RCGECRRIFDMQVSAFESLQKSQS"
     gene            complement(502..837)
                     /locus_tag="PLS35_15475"
     CDS             complement(502..837)
                     /locus_tag="PLS35_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20123.1"
                     /translation="MTLLLDPQQRSTRPAWHVFFSHTLRYVLAPLLFISSGFFTANFI
                     VSAHYTWPRTSRVFVLTLTLLILAYEFIYKDRTIQSNAPDRAFNAVLYSCMIPYALGV
                     LMMLALAKL"
     gene            1419..2405
                     /locus_tag="PLS35_15480"
     CDS             1419..2405
                     /locus_tag="PLS35_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNI20124.1"
                     /translation="MLAEVSFIDTPSTLLLLSAGSHPFIVQASYLPMNIKDYLEQKRL
                     AVDRFLDEVSPPAATPPTTLHESMRYSLMAGGKRVRPILTIAAAEAVGTTPPGLMAVA
                     CSLEFIHTYSLIHDDLPSMDNDDFRRGKPTNHKVYGEAMAILAGDALLTMAFDLISRP
                     ELMKGCEPIRQVRILQELAYGSGNMGMVGGQVFDIQAENQDIDLPTLQNIHKHKTGML
                     IRAAVRMGAIAAGATDRQLDDMTGYAEDIGLAFQIADDVLNVTGTREELGKNPNTDAE
                     RGKKTYPTFYGVEGARKLADDCVTRAIGRLNTFGSSADPLREIARYITSRKN"
     gene            2514..3497
                     /locus_tag="PLS35_15485"
     CDS             2514..3497
                     /locus_tag="PLS35_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNI20125.1"
                     /translation="MKALVTGATGFVGGAVARALVKAGVEVRVISRTGADLQNLAGLP
                     VEQVQGDLRDRDSLREALRGCRQLYHVAAHYALWAKDPSIFYDINVTGTRTLLETARE
                     VGIERTVYCSTIGAIGLPPGGGLGTEETPVSLEQMAGHYKRSKYLAEQEVLKLAREGL
                     PVVIVNPSAPVGEADVKPTPTGQIIVDFMKDRMPAYIETGMNLVDVDDVAQGHLLAMQ
                     KGRQGERYILGNKNLLLNEVFQILSRITGVKAPSVKLPRLAILPLAYANQWIANLTGV
                     PPRIPLEGVKMAKYKMHYDCSKAIRELGLPQTPVEVALEKAVRWFREHGYA"
     gene            3633..4442
                     /locus_tag="PLS35_15490"
     CDS             3633..4442
                     /locus_tag="PLS35_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNI20126.1"
                     /translation="MEFFFLFLKTIWFRPYVFAFLAAFLFSAIMLIGWSRTWRFWLIS
                     WITAFVCEYSSTRNGIPFGWYHYNGSTIGQELYISNIPFMDSISFSFLLFASYCLALL
                     LLLPMAPGQRNPGFQLPRLTFALGDRTSWQVFALTVLFFAFIDMVIDPVALRGDRWFL
                     GKIYYYPNPGVHYGVPMANYAGWAVVGAISLFIYFPLDRRLATTLPAQTPSTTHRLLL
                     GAGLYYGVLIFNLAVTFWIGESLQGTTGVLMYVPVTAILLLRLLPPAPAAA"
     gene            4549..5166
                     /locus_tag="PLS35_15495"
     CDS             4549..5166
                     /locus_tag="PLS35_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI20127.1"
                     /translation="MKKLCGLLLLGFLATLGILGWFGYQSYSTGFSAKAEPNELEVLL
                     ARQLRHLAIPYENRRLRNPLPVTQELMKEARAHFADHCASCHANNGSGDTVIGRNVYP
                     KSPDLRLPDTQTMSDGELFFIIQNGIRFTAMPGWGTGDPAKDSGSWQLVHFIRHLPSI
                     TEEELREMAALNPKTKQELQDEALIDQFLGGDDAAASSATGAHRH"
     gene            5190..5510
                     /locus_tag="PLS35_15500"
     CDS             5190..5510
                     /locus_tag="PLS35_15500"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNI20128.1"
                     /translation="MTFLRLFIVALLLICAPAVVSAHGSGQHVLGVVSAIDATHIEIK
                     TPKGQIVSVRLTDKTQYKVRNLRRPKSPPQVGDRVVVEAEKGTDGLTATEVHYSDSQP
                     KAAQ"
     gene            5517..5990
                     /locus_tag="PLS35_15505"
     CDS             5517..5990
                     /locus_tag="PLS35_15505"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNI20129.1"
                     /translation="MRVFQLSHNQQTGPGGDGLRPLVIRALIMGLAVFLAVTIVPGIE
                     SDSLGAGVAAVLVLTLLNSLIRPLLYLLALPLIVVSLGLFMVVINALLLQLTAALVKG
                     FTVAGFGASFWGALVISLVSSILNMLLVVEQSRVEPSHRPQRPPTIINPDGSEHS"
     gene            6190..8019
                     /locus_tag="PLS35_15510"
     CDS             6190..8019
                     /locus_tag="PLS35_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20130.1"
                     /translation="MSPPVEPAKATAETHLQRTLTSVLNGLPADAALVAIFHQEQGPL
                     TTQVHRGFTPRDVQSIGRTLSSQKVLTAVPVGTDPEASRTMRLRLITPGAKSLLGVPL
                     RHRNRTYGFLVIGRKDNAVYAKKDKTMLEQAGDDITKALERDNLFDLNVLLSRPLVVQ
                     EPQPVIAAPDVYTPPTSHATPEIQGKIVTLLNELSQTLPFDRAWIGSYDPLAGNVEVL
                     GIAGEQKTDPKDQKKDLKAGQRLTLDASAAGWVVRHRKPRVDHDLASTQGRFLDHKHL
                     YKDRFQSSLVLPFFLRGQVGGTITLASKEADRYAVTDARLLEPINLKLVDLLQAAPPA
                     ATGAVKAEGAPDSEAGTTALTSAEPVIRKQERQAAIGEFSAFLATEIREPLGSIRAQL
                     EEVTAEGILDFDPQTRVENAMRDLIRIEAILNEILDFAKPLDLTRRLCRVPEMVENAL
                     TVVATELEATRIQVVKEYPQLLAPVRADEAKMQQVFLSIFKNAIEAMTPGGILTITMS
                     NQRAGKHLEVQILIKNNGAPIPPEHIDKVFEPFFTTKRSGTGLGLATVKKIVEEHQGS
                     IGISGAPGEGTTVTIRLPGVSRGPAHRYRGRGRRPPRRPTAAS"
BASE COUNT         1717 a         2549 c         2277 g         1613 t
ORIGIN      
        1 cgattgggat ttctgaagag attcaaacgc tgagacctgc atgtcaaata tgcgccggca
       61 ttcaccacag cgcaatccca cctggtcgcg aatcacatcc aattccctaa tggtgacgga
      121 gacgggatgg gcacgcagac agtcttccag tgattccctc ggcgtcggta cggctttggg
      181 actctccact tcgccgccaa gtggcacagc cgcaactcgc cccacgacct gatcaggcgt
      241 cataagatgc atcatgcgac aggacgtgca ggcgatcacc aggcgtccgt cgtcgtcaat
      301 ccgcttgagc gacaaacata agggatggac ggcgaagcat tgttggacaa aggcgccgct
      361 ttgaaaccgc tgtgccatag ccacctattg aaggatgagg gacgccggtg cctgctggct
      421 cttcagaacg tgatcgatag gatgttcacg acacggttca gcgaagcgag acaaccgccg
      481 tgggtggttt tcaacatcct ttcacagctt ggccaacgcc agcatcatca acacaccgag
      541 agcatacgga atcatacagg aatacagcac cgcattgaat gcccgatccg gggcattcga
      601 ctgaatggtg cgatctttgt aaatgaactc gtaggcaaga atcagcaggg tgagggtcag
      661 gacgaagacc cggctcgtgc gaggccaggt gtaatgagcg ctcacgatga aatttgccgt
      721 aaagaatccg ctggaaatga agagcagtgg cgcgagcaca taccgcagcg tgtgcgaaaa
      781 gaacacgtgc caggccggcc tggtcgaacg ctgctgcgga tctaatagca gggtcatgac
      841 ccgacaggcg cgggttggct ggacggagac gaaggggctt gtttggcttg ctctgcagca
      901 gttttgcagg ttttgcaaat acgcgggcgc tgtttgagga aactgatttt tccgctcgcg
      961 aggcagctat agtggacgaa ttcgtcgcag accgcgcacc gtgaccatcc cccgcgaagc
     1021 atcgtcttgt cgcggtacag gccctggtta cacaccttgc agttgcgggg ttcaatcccc
     1081 aacagcatcg gctcccagtc accaccgcca ttgcgaagaa tattcatggg gcggaagtat
     1141 aacgaagggg tcgaagaaaa aacaaggagg gaggggtagg agggcggccc tagtggtccc
     1201 cccatgatcg cgcaacgcgc ggcctgggaa ggccctcgtt ggacgcgctc actctgggag
     1261 gaccaccaga ccgccctcct aacaagatag acgtaagaag aaggcgcacg atcggaatgt
     1321 gcccgtccca tgcgcagtga agcgccacca ggcgagtccc tcggtaagag acgtaggcat
     1381 gatcgtatgc gcaagccacg cgttccacga gcaggaggat gctggcagaa gtcagcttca
     1441 ttgacacccc ttcaaccctt ttgctactct ccgccggttc ccatccattc atcgtccagg
     1501 cttcgtatct acccatgaac attaaagact atctcgaaca gaagcggctc gcagtggacc
     1561 gttttctcga tgaggtcagt cctcccgctg ccacaccgcc cacgacgctc catgagagca
     1621 tgcggtacag cttgatggcc ggcggcaagc gcgttcgacc gatcctcacc atcgccgccg
     1681 ccgaggcggt cggaaccaca ccgccggggc tgatggccgt ggcctgttcg ctggaattca
     1741 tccataccta ttccctgatt cacgacgatt taccctcgat ggacaacgac gactttcgcc
     1801 ggggcaagcc gaccaatcac aaggtctacg gcgaagcgat ggcgattctg gccggcgacg
     1861 cactgctgac catggccttc gatctcatca gccggcccga actcatgaaa ggctgtgagc
     1921 ccatccgtca ggtgcgcatc cttcaggagc tggcttacgg atccggcaac atgggcatgg
     1981 tgggcggcca ggtgttcgac atccaggccg agaatcagga catcgatctc cccacgctac
     2041 agaatatcca taaacataaa accggcatgt tgatccgggc cgccgtgcgc atgggcgcga
     2101 ttgcggccgg cgcgacggat cggcagctcg atgacatgac cggctatgcc gaagacatcg
     2161 gcttggcctt tcagatcgcc gacgatgtgc tgaatgtcac cggtacccgc gaggaactcg
     2221 gcaagaatcc caatactgat gccgagcgcg ggaagaagac ctatccgacg ttttatggcg
     2281 tcgaaggcgc gcgcaagctg gcggacgatt gtgtgacccg cgcaatcggc cggctcaaca
     2341 cattcggctc ctcagccgat ccgctccgcg agatcgcgcg ctatatcacc tcgcgaaaga
     2401 actgacctga ggcgtgaaac gtaagacgtg agacgcaatc ccttcgatcg cgcgcccctc
     2461 tcacgtcacc cacggttcac cgttcacgct gtccgcttca cggaaattca ggcatgaaag
     2521 ctctcgtaac cggagcgacc gggtttgtcg gcggggccgt cgcacgcgcg ctggtgaaag
     2581 ccggggtaga agtccgggtc atctcccgaa ccggtgctga tctgcagaac ctggccgggc
     2641 tcccggtcga acaggtgcag ggcgacctcc gcgatcgtga ttcacttcgt gaagcattac
     2701 gcggctgccg gcagctctat cacgtagcgg cgcattacgc gctatgggcc aaagatccct
     2761 ccatcttcta cgatatcaat gtcaccggca ccaggacgct gcttgagacg gcccgtgagg
     2821 tcggcatcga acgtaccgtc tattgcagca ccatcggtgc gatcggcttg ccgccgggcg
     2881 gcggcttagg cactgaggag acgccggtgt ccctcgaaca gatggcgggg cattacaaac
     2941 gatccaaata tctcgccgaa caggaagtcc tgaaactggc acgcgaaggc ttgccggtcg
     3001 tcatcgtgaa ccccagcgcg ccggtcgggg aagcagatgt aaaaccgaca cccaccggcc
     3061 agatcatcgt agacttcatg aaggaccgga tgcctgccta tatcgaaacg ggcatgaacc
     3121 tggtcgatgt ggatgacgtg gcgcagggcc atctgctcgc catgcagaaa ggccggcagg
     3181 gcgaacgcta cattctcggc aataagaatt tgttactcaa tgaagtattt cagattctca
     3241 gccggattac gggcgtcaag gcgccctcag tgaaactccc tcgcctggcc attctgccgt
     3301 tggcctatgc gaatcaatgg atcgcgaacc tgacgggcgt gccaccccgc atcccgctcg
     3361 aaggcgtgaa gatggccaaa tacaaaatgc attacgattg ttcgaaggcc attcgcgaac
     3421 tgggtctgcc tcaaacaccg gtcgaggtcg cgctagagaa agcggtgcgg tggtttcgcg
     3481 agcatggcta tgcttaacgg atgctgaaaa aggctttgag ctgcgttctc ggctcgacaa
     3541 gatcctcaac gtaccctcaa gggtacgcct ccggtcttgc cagcgcctgc ggccttgcta
     3601 aaagaccttt ttgagcatcc tccacttgcc cgatggaatt cttctttctg tttctcaaaa
     3661 cgatctggtt tcgcccctac gtctttgcct ttctggcggc gtttttgttt tcagcgatca
     3721 tgctgatcgg ctggtcgcgg acctggcgat tctggctcat cagctggatc accgcattcg
     3781 tgtgcgagta ttcctcaacg cgtaacggca ttcccttcgg ctggtatcac tacaacggct
     3841 cgacgatcgg acaagagctg tacatttcca acattccatt catggattcg atctcgtttt
     3901 cgttcctcct gttcgccagc tactgcctcg ctcttctctt gcttctgcct atggcaccgg
     3961 gccagcgcaa ccccggtttc cagctccccc ggttgacgtt tgcgctcggc gaccgaacgt
     4021 catggcaagt ctttgccctg accgtcctgt tctttgcctt tattgatatg gtgatcgatc
     4081 cggtggcgct tcgcggcgac cgctggtttc tgggcaagat ctattattac ccgaaccccg
     4141 gcgtccatta cggcgtcccc atggctaact acgccggatg ggccgttgtc ggcgccatct
     4201 ccctcttcat ctactttccg ctcgaccgcc gcctcgccac cacacttccg gcccagacgc
     4261 cttccaccac acatcgcctc ttgctgggcg caggcctcta ctacggcgtg ttgatcttca
     4321 acctggccgt caccttctgg atcggcgagt cactgcaggg cacgaccggc gtcttgatgt
     4381 atgtgccggt gaccgccatc cttttgctcc gattactccc ccctgcacct gcggccgcat
     4441 aaatcacgcg ctcctgcctg tggcaaaagg ggcgcaattg gcattcattg gaatgagact
     4501 gtatagtggt aaacaggcgc gatgcctgcg cgggaaggct tgtctctgat gaaaaaactc
     4561 tgcggactgt tgttgctcgg cttcctcgcg accctcggca ttctcgggtg gttcggctac
     4621 caatcctact cgaccggatt tagcgccaag gccgaaccga acgaattgga agtcctgctt
     4681 gccagacaac tgcgacacct ggccattccc tatgagaatc gacgactccg caaccccctg
     4741 cccgtcacgc aagagttgat gaaggaagct cgcgcccatt tcgccgacca ttgcgcttcc
     4801 tgccatgcca acaacggcag cggggacacg gtgatcggca ggaatgtcta ccccaagtct
     4861 ccggatctcc gcctgccgga tacgcagacg atgtcggacg gtgagctgtt ctttatcatt
     4921 cagaacggca tccggttcac cgccatgccg ggctggggca ccggcgatcc ggcgaaggac
     4981 agcggaagct ggcaactggt gcatttcatc cgccacctgc ccagcatcac ggaggaagag
     5041 ctgcgggaaa tggccgcgtt gaaccctaaa acgaagcaag aattgcaaga cgaagctctc
     5101 atcgatcagt ttctgggcgg agacgatgcc gcggcatcca gtgccaccgg cgcacaccgc
     5161 cattaatgct ccaacgaaag gattttcccg tgacattcct tcgcctcttc attgttgcac
     5221 tcctcctcat ctgcgccccg gctgtcgtat ccgcccatgg cagcggccag catgtgctcg
     5281 gggtcgtctc agccatcgat gccacccata tcgaaatcaa gactcccaaa ggccagatcg
     5341 tctcggttcg tctgacggac aagactcaat acaaagtgcg aaatctgcgc cgcccgaaaa
     5401 gtccgcctca agtgggagac cgggtcgtcg tcgaagcgga gaaagggact gacggtctga
     5461 cggcgacgga agtccattat tccgattccc aaccgaaggc ggcccaataa ctcaccatgc
     5521 gcgtgtttca actctcgcac aaccagcaga ccggacctgg cggcgacggt cttcggccct
     5581 tggtgatcag agctttgatc atgggcctcg ccgtcttcct ggccgtgacc attgtgcctg
     5641 gcatcgagtc cgacagtctc ggtgccggcg tcgccgccgt gctggtactg acgctgctga
     5701 acagcctgat tcgcccgctc ctgtatctgc tggcgcttcc gctcatcgtc gtgtcgctcg
     5761 ggctgttcat ggttgtcatc aacgccttgt tactgcaatt gacggctgcg ttggtcaaag
     5821 ggttcaccgt cgccgggttc ggcgcatcgt tctggggcgc cctcgtcatc agtctggtca
     5881 gcagtattct caacatgctg ctggtggtgg aacagagccg ggtcgagccg agccatcgcc
     5941 ctcaacgccc gccgaccatc atcaatcccg acgggtcaga gcactcgtga acgctgcgac
     6001 tttccttgag ttactctgat atgactgtta caatgcgccc cgacgctcga tcttcaccgc
     6061 agagagcgca caacctataa cgagcatcgt gtttcgatcg tgtagcgggt ctgattcacg
     6121 gtcagcgcaa agacgccgat cgacaatccc cgccccctgc atcatcggaa acatcctgca
     6181 cagaggtcga tgagtccccc agtcgaacca gcgaaagcca cagccgaaac ccatcttcag
     6241 cgcaccctga cctccgtcct caacggattg ccggccgatg ccgcgctggt cgcgattttc
     6301 caccaggaac agggcccact cacgacgcag gtgcaccggg gctttacgcc gcgggacgtt
     6361 caatcgatcg gccgcacgtt gtcgtcccag aaggtgttga cggccgtgcc ggtcgggact
     6421 gaccccgaag cgtcgcgcac catgcgcctt cggctgatta ctcctggagc caagtcgctg
     6481 ctgggcgtgc cgctccgcca ccgcaaccgc acctatgggt ttctggtgat cggacgcaaa
     6541 gacaatgcgg tctatgcaaa aaaagacaag accatgctcg agcaagccgg ggacgacatt
     6601 acgaaggccc tcgagcgcga caaccttttc gatctgaatg tgctcctcag ccgaccactg
     6661 gtggtgcagg aaccgcaacc ggtcattgcc gcaccggatg tctacactcc gccgacttcc
     6721 cacgcgacgc cggagatcca gggcaagatc gtcaccctgc tcaatgagtt gagccagacc
     6781 ctgccgttcg atcgcgcctg gatcggttcc tatgatccgc tcgccggcaa cgtcgaagta
     6841 ctgggcatcg ccggggaaca aaagaccgat cccaaagacc agaaaaagga tctcaaagct
     6901 ggccagcgac tgacgctcga tgcctccgct gccggctggg tggtgcgtca ccggaagccc
     6961 cgggtcgacc atgacctcgc ttccacgcaa ggccgctttc tcgatcacaa gcatttgtat
     7021 aaagaccgct tccaatcttc attggtattg cccttcttct tgcgcggcca agtcggtgga
     7081 accatcacgc tggcctcgaa ggaagcagac cgctatgcgg tgaccgatgc tcgcttgctc
     7141 gagccgatca acctcaaatt ggtggatttg ctccaggctg cgcccccggc ggcaaccggc
     7201 gccgtcaaag ccgaaggcgc gccggattcc gaggccggga ccacagcatt gacctccgcc
     7261 gagccggtca tcagaaagca ggagcggcag gctgctatcg gggaattcag cgcgtttctg
     7321 gccactgaaa tccgtgagcc attgggctcg atccgcgcgc agttggaaga agtcacggcg
     7381 gaaggcattc tcgatttcga cccgcagacc cgtgtcgaga acgcgatgcg cgacctgatc
     7441 cgcatcgaag cgatcttgaa tgaaatcctc gatttcgcca aaccgctgga cctcacgcgc
     7501 cgcctctgtc gcgtccccga aatggtggaa aacgcgttaa ccgtggtggc caccgaactg
     7561 gaagccaccc gcattcaagt cgtcaaagaa tatccccagc tcctcgcgcc ggtgcgcgcc
     7621 gatgaagcga agatgcagca ggtcttcctc agcattttca aaaatgcgat cgaggccatg
     7681 accccgggcg gcatcctgac gatcaccatg agtaaccagc gggccggcaa acatctggaa
     7741 gtccagatcc tgatcaagaa taacggggca ccgatcccgc ccgaacatat cgacaaggtt
     7801 ttcgagccgt tctttacgac gaagcgttcc ggcaccggcc tcggtctcgc cacggtcaag
     7861 aagatcgtcg aggagcatca gggcagcatc ggcatttccg gcgcaccagg cgaaggcaca
     7921 accgtgacga ttcgcctgcc cggcgtcagc cgtgggcctg cgcaccgcta tcgtggccgc
     7981 ggacgacgcc cgccgcgtcg ccccaccgcc gcatcgtagc tgctgacctc caaggattcg
     8041 atccgaaggt tagacctgaa gcgaatcttt tcagtgcgcc tacgcctgac atcaaacaac
     8101 acaatattct cgttccagac gcgtgcacgc cggcctggca tgccatcacc ccagcc
//
LOCUS       DAOQEW010000174         8123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3049_length_8123_cov_6.820897, whole genome shotgun sequence.
ACCESSION   DAOQEW010000174 DAOQEW010000000
VERSION     DAOQEW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3049_length_8123_cov_6.820897"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLS35_15515"
     CDS             complement(<1..1001)
                     /locus_tag="PLS35_15515"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNI20131.1"
                     /translation="MFRAITKWANDKNGSRVFLIAGGPGTGKTAIAARVVQMHLGDVL
                     GELSRGCLSSFHFCQAGLDSTLSPLTFVQSLAEALANRYSVYRAALEKQGTQQFVIHS
                     SVTVQGDVAANAQIAGIDIGHVQINITGGDARPLFDQMVRRPLQELCEQSPTQSIVIL
                     VDSLDEALTFNPDTNIAQLLNLVQDFPKQVRFLLTSRSNNERVFDLVGQPTLDLSTDT
                     PPGLDEVKAYATARLAEAPGPERSVVIDCIAQQSKGNFLYAYYVLNDLLRPGVALNRA
                     AVKDLPVALEDVYRKFLERTVASNPTRWKDVYRPLLGLIAVARGQGLEKPQLIAMTGL
                     "
     gene            complement(896..1780)
                     /locus_tag="PLS35_15520"
     CDS             complement(896..1780)
                     /locus_tag="PLS35_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20132.1"
                     /translation="MTRLPLRIAKHIDQFTGRTWLLPKLLDWFNNRETERLFLLTGGP
                     GSGKSMVMAWLAGHGPIPVDGTAQQQLAEIRAEVKACHFCVADSGNTDPKEVARQIAD
                     QLTRTVPGFGEALAATLPDQVRINSVLKDVTVQQGGSVTGVYIGYFNLQGLSEELSFN
                     RVLRDPLQQLYRRDGYDEPMLLLFDALDEALTYSGKTHIVHLLQKFSDLPPNVRILAT
                     TRDVPQVTNEVKKWRRPYFLMRRSTLLHSLRNAHSTLRAVIGCSGPSLSGQMTKMVLG
                     CSSSPVVQEQERLRSLPE"
     gene            complement(1786..2565)
                     /locus_tag="PLS35_15525"
     CDS             complement(1786..2565)
                     /locus_tag="PLS35_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20133.1"
                     /translation="MHEDMIVGAQEFVTMVDLPEPPPLSARAAKDIFDYDSAKQQALV
                     VGSDVIAFVKGITPEQRTDLVNASLLAQLAAKKKVANPKTLKAVEQWYNEYFDALSRI
                     GFLIHDKGLAKYEEASESFEAHEAILDVATTLLAGIPGALALVTTTLKSLQKMSANSP
                     WITLFNRESRSANTARFQMSLVSEDGQGGLLVSLIAFGMEAKTTLTQVLFLKFHTNEV
                     KLHHHSGQVTINAPLLAAVREQIADKLAAYVSDYIKGLPDL"
     gene            complement(2634..3635)
                     /locus_tag="PLS35_15530"
     CDS             complement(2634..3635)
                     /locus_tag="PLS35_15530"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI20134.1"
                     /translation="MFEHGYALLIGVDQNRTPNLALPTVKKDVTKLKEVLTHPDRCGY
                     LEENVRVVTGMGATRDKILDGLDWLRDKVAADKDQNQTALVYYSGHGHREGSGESFFI
                     PYDVRFPIRTGALPAKDFAEILADINPRRLFVILDCCHAEGMNVKDASAQHVASAAVT
                     KETPGMGRLAEGDGRVILSSSRGTQQSWIRSDGEMSVFTYHLAEALIGHAGRPAEPDV
                     TVTEVMDYVARSVPKTAREQHDAEQQPFFQYSGSSFPIALVIGGKGIAKGMTPPDPLA
                     ELPHVKATMNLKELEGEATNVDIDEMNRGNLEANAAGETIREGGKFTNLKINKLGKR"
     gene            complement(3747..5447)
                     /locus_tag="PLS35_15535"
     CDS             complement(3747..5447)
                     /locus_tag="PLS35_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20135.1"
                     /translation="MAKREAVKNGLERQRLIVVGADVLAFGEDITLEQRGDILNASLL
                     AQLVAKERVLNPNALADWYKEYFDSLSQVGLVFHGTTNMKSCNATDNLEVQEAILGVA
                     AEGLVDSPRSLAMVKAIFKSLQTLPSTSPPMALFNRESCSANTARFRLILVDKDEHGR
                     HLVTLMAFGIEAQKTLTQLLSLKLNKREAGIYHYVGQVPMVPTLSAAVRDQMANKLAA
                     FSTNYISGIPVLGSEGVRNGSEAADPLTELPAMVNATMKVDRLDGEATNVEIDEMSSG
                     QVDATTIVQTVTGNLTGVKIGTLGSTRVPDMIRSTTSGSGQELQELIEKYFTASQHRE
                     NASNHKTPAKRTSLKEFSTTTANVHVDRVDRGQVIGSQVNRRDGDRIQGDQVNVASGG
                     QASNHTGFSGSVKINGPVTIIQGPTGSHPHANTGSTVNDSLTIEEKIRLDVALPKNTL
                     VNEPFDVVIAVKQPDAPVLAVTDLDQVVSGDGSVIRSEADDVVKYRVEVTGADVEVIP
                     KSYVIELRPKTNSRPIAFQVVSSKKGRRSLIVNAYQENEALVAQTRVTIEVVIAVSTN
                     "
     gene            complement(5634..6833)
                     /locus_tag="PLS35_15540"
     CDS             complement(5634..6833)
                     /locus_tag="PLS35_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNI20136.1"
                     /translation="MVKYDLVFEGGGAKGMVFVGACEEFFRRGHTFNRLLGTSAGAIT
                     ATLLAAGYTPEEMLAALVEKDPEGKSVFTSFMGPPASFSKAELRGSATKRLLEGVDFT
                     VIPDFIEKKIDEMILDAMANGGAFRHILALIERGGWFAADRFVTWLSSKLDSGSTTVG
                     PRQFSKMTLSQFYAATNIELSLVASDTTDESLLVLNHRTAPKCPIVWAVRMSMSIPLV
                     WDEIIWKANWGQYLGRDMEGHAIVDGGILSNFPIELFISDALQVTKLMGPKQDTPVLG
                     LLIDEKLPVSLAKGIFVNITIKPGELQTVQRLRRLVDTATGAHDKMVMDEYSHLVVRL
                     PAQGYGTTEFDMTDERRNALVKAGQGAMARYFDMPTNVAQAPGGFSKGGIVWGSPADR
                     IATAILQ"
     gene            7820..>8123
                     /locus_tag="PLS35_15545"
     CDS             7820..>8123
                     /locus_tag="PLS35_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20137.1"
                     /translation="MVIQVPKKVVDNIDQFHGREWLLPKVLEWWHKGKDRLFLLTGEP
                     GTGKSMIIGWLGGFGNPPINVTPKAELAQVRTLIKAVHFCQAASRNVTPQAFADSMA"
BASE COUNT         1843 a         2244 c         1978 g         2058 t
ORIGIN      
        1 agccccgtca tcgcgatcag ttgcggtttt tcaagtcctt gaccgcgcgc cacagcaatc
       61 agaccgagga gcgggcgata gacatctttc cagcgcgtgg ggtttgatgc aacggtacgt
      121 tcaagaaatt tccgatacac atcctccagc gccacgggca aatccttcac tgcggcacga
      181 ttgagtgcaa ccccaggacg aagcagatcg ttgagcacgt agtaggcata gagaaaattg
      241 cccttgctct gttgggcgat gcaatcgatc actactgatc gctccggccc cggtgcctcg
      301 gctagccgtg cggtggcgta ggccttcact tcatcgagcc ctggtggtgt atcggtactc
      361 aaatccagtg tcggctgacc gacgagatca aacacccgct cattgttcga ccggcttgtg
      421 agaagaaaac gcacttgctt cgggaaatcc tgcacgagat ttagcaactg cgcgatattg
      481 gtgtcgggat tgaaggtgag agcttcgtcg agggaatcga caaggatcac gatcgattgg
      541 gtgggactct gctcgcacaa ctcctggagg gggcgtcgca ccatttggtc gaacagcggc
      601 cgggcgtctc cgcccgtgat attgatctga acgtgcccga tgtcgattcc agctatctgc
      661 gcattagcgg caacatctcc ctggaccgtg acggacgaat gaatgacgaa ctgctgagtg
      721 ccctgctttt ccaacgcagc ccggtacacg gaatagcgat tggcaagtgc ctccgccaat
      781 gattgcacaa aggtgagagg cgacagcgtt gagtccagac ccgcttgaca gaagtggaaa
      841 ctcgagagac agccacgaga caattcaccc agaacgtctc ccaggtgcat ttgcactact
      901 cgggcagcga tcgcagtctt tcctgttcct ggaccaccgg cgatgaggaa cacccgagaa
      961 ccatttttgt catttgccca cttagtgatg gccctgaaca cccaatcacg gcccgtaaag
     1021 tgctgtgtgc gttcctcaat gagtggagta aagttgagcg gcgcatgagg aagtacggtc
     1081 ttcgccattt tttcacctcg ttcgtcacct gtggtacatc ccgagtggtg gccaagatac
     1141 gaacattagg aggcagatca ctgaactttt gtaacaggtg tacgatatgt gtcttgccgg
     1201 aataggtcaa cgcctcatcg agcgcatcga agagaagcaa catcggctcg tcatatccgt
     1261 ctcttctata aagttgctgg agcggatctc gcaggacacg gttaaaactc agctcttcgc
     1321 ttaggccctg caggttgaaa tagccaatat agacgccggt gacgctacca ccctgttgaa
     1381 ccgtgacgtc tttcagtacg ctattgatac gcacttggtc gggcaacgtt gcggccaacg
     1441 cctccccaaa cccgggcacc gtgcgcgtca gctgatccgc gatttgtcga gcaacttcct
     1501 tggggtctgt gtttccactg tccgcgacac aaaaatggca ggccttaacc tcagcacgta
     1561 tctctgcgag ttgttgctgc gctgtgccat ccaccggtat cggcccatgc cccgctagcc
     1621 aggccatgac catgctcttt ccggacccgg gaccaccggt cagcaagaag aggcgttctg
     1681 tttctctgtt gttaaaccag tcgaggagct tcggcaacag ccacgtgcgg ccagtgaatt
     1741 ggtcgatgtg tttagcgata cgtaatggga gtcgagtcat tcgatttaga gatccggcaa
     1801 gcccttgatg tagtcgctta cataggcagc gagtttgtca gcaatctgct cacgcacagc
     1861 tgcaagtagc ggcgcattga tggtgacctg ccccgagtga tgatggagct tcacctcatt
     1921 tgtatggaat ttaagaaaga gcacttgtgt cagcgtggtc ttagcttcca taccaaacgc
     1981 gatgaggctc actaggaggc cgccctgccc gtcttcacta actagcgaca tctgaaagcg
     2041 agccgtgttc gctgatcggc tttcacgatt gaacaacgtg atccatgggc tgtttgccga
     2101 catcttctgc aaggatttca gggtcgtggt cacaagcgcc aacgcaccag gtatcccagc
     2161 cagtaatgtg gtggcgacgt ccaagatggc ctcgtgcgct tcgaaactct cactcgcttc
     2221 ttcatactta gccaagccct tgtcatgaat taaaaagcca atccgtgaaa gggcatcaaa
     2281 atattcgttg taccactgtt ccacggcttt aagagttttt ggattggcga ccttcttttt
     2341 tgccgccagc tgtgcaagga gagaggcatt cacaaggtcc gtgcgctgct ccggtgtaat
     2401 acccttcacg aaggcaatca catcggaacc aacgactaac gcctgctgct ttgccgagtc
     2461 ataatcaaaa atatccttcg ctgctcgtgc actcagcggt ggcggctccg gcaggtcaac
     2521 catcgtgacg aattcttgag ctccaacaat catatcttcg tgcattctct cctcctaacc
     2581 tggccccatc cacccacctg aatactcttc ggttgctaat tgcaccagct ccatcaccgc
     2641 tttccgagct tgttgatctt gagattcgtg aattttccac cttctcggat cgtttctcct
     2701 gcggcgtttg cttcaagatt gccccgattc atttcatcta tatcgacatt cgttgcctca
     2761 ccttccagct ctttgagatt catggtggct ttaacatgtg gaagctcggc gagcggatcg
     2821 ggcggtgtca tcccttttgc aatgcctttg cccccgatga ctagtgctat cggaaaggac
     2881 gatccagaat actgaaagaa aggctgttgt tcagcgtcgt gttgctcacg agctgtcttt
     2941 ggcacagacc gagccacata atccatcact tcggttacgg tcacatccgg ttccgcaggg
     3001 cgacccgcat ggccaatgag tgcctctgcc agatgatatg tgaagacgct catctcgcca
     3061 tcgctccgaa tccaggactg ttgagtgccg cgggaggagc tgagaatcac acgaccatcg
     3121 ccttccgcga gtctccccat acccggagtc tccttagtta ccgcggccga tgctacatgc
     3181 tgcgctgaag catctttcac gttcatgccc tcggcatgac agcagtcgag aatgacaaag
     3241 aggcgccgag ggttgatatc ggctagaatc tcggcaaagt ctttcgcggg caaggccccg
     3301 gtacggattg gaaaccgcac gtcataagga atgaaaaacg attctccaga cccctcacga
     3361 tggccgtgcc cggaataata gaccaacgcg gtttggttct ggtctttgtc ggccgcgacc
     3421 ttatctctca gccaatctag gccatcgaga atcttatcgc gtgtcgcgcc catgccagta
     3481 acaactcgaa cattctcttc cagatatccg cagcggtccg gatgcgtcag cacttccttg
     3541 agtttggtga catctttctt gacggtcggc aatgcgagat tcggggttct gttctgatct
     3601 acaccaatca atagtgcata gccatgctca aacataagcc ctccattcgc aagatattct
     3661 tgtcgtcatt atcatgtcag tcaatacagt agtggcactt ctggcacctc tgcagtcatt
     3721 atgcaaattg caccagctac acacccctag tttgtggata cggcaatcac gacttcgatt
     3781 gtcactcgtg tctgggcgac aagcgcctca ttctcttgat acgcgttcac gatcaacgac
     3841 cgcctcccct tcttgctcga tacgacttga aacgcgatcg gccgtgagtt tgttttgggt
     3901 cgcagctcga tcacataact cttcggtatt acctcaacgt ctgcgccggt tacttcgaca
     3961 cgatatttca caacgtcgtc cgcctcgcta cgaatgacgc tcccgtcgcc ggaaacaacc
     4021 tgatcgaggt cggttaccgc aagcaccggc gcgtctggtt gtttcaccgc aatcaccaca
     4081 tcgaatggtt cgttaacgag agtgttcttg ggcagcgcca catcgagtcg tatcttttcc
     4141 tcaatagtga gtgagtcatt caccgtggaa cccgtattgg cgtgtgggtg gcttccagtt
     4201 ggtccctgaa tgatcgtgac ggggccgttg atttttactg aaccactgaa ccctgtatga
     4261 ttggatgctt gtccacccga ggcgacattc acttgatccc cctgaatcct atcgccatcc
     4321 cgccggttca cttgggagcc aattacctgt cctctgtcaa cgcgatccac atggacattt
     4381 gccgtcgtcg tggagaattc ttttagcgaa gtacgtttag ccggagtttt gtgattcgaa
     4441 gcattttccc tgtgttggct cgctgtgaaa tatttctcaa ttaattcttg cagttcctgc
     4501 cctgaacccg atgttgtcga cctaatcatg tcaggtactc gggtcgatcc caacgtacca
     4561 attttgacgc ccgtcaggtt tcccgtaact gtctgtacta tggttgttgc atccacttgg
     4621 ccactactca tctcgtcgat ctctacattt gttgcttccc cgtcaagacg gtcgaccttc
     4681 atcgtggcgt taaccatggc gggaagctcg gttagtggat cagctgcttc agatccgttc
     4741 cttacacctt cgctacctag cactggtatt ccactgatgt aattggtcga aaaagccgct
     4801 agcttgttgg ccatctgatc gcgcacagct gctgatagag ttgggaccat cggcacttgg
     4861 cccacgtaat gatagatccc cgcctccctt ttattaagct ttaaagagag taattgtgtg
     4921 agtgtctttt gagcctcaat tccaaacgcc atgagggtca ccaaatgccg accatgctcg
     4981 tctttgtcta ccaatatcaa acgaaaccgg gcggtgttcg ctgagcagct ttcgcgatta
     5041 aacaaagcca ttgggggact ggtggaaggc aacgtctgca aggacttaaa aatggctttg
     5101 accatcgcca gcgatctcgg cgagtctact aatccctccg ccgcaactcc gaggatagcc
     5161 tcttgcactt caagattgtc agtagcattg caagatttca tattcgttgt gccgtgaaac
     5221 acaagaccta cctgagagag gctatcaaag tattctttgt accagtctgc aagtgcgttt
     5281 ggattgagca ctctttcctt ggccacgagc tgcgcgagca atgatgcatt gaggatgtcg
     5341 ccgcgctgtt ccagcgtgat atcttcgccg aatgcaagca cgtcagcacc cacaactatt
     5401 aatcgttgtc tctctaaacc atttttaacg gcttcccgtt tagccatagt tgaattttac
     5461 ctccgaaccc gtatctaact accttacaga tctcaagcct gccgcaacca gacagctgct
     5521 cctctgcacc acttctaacg accgttcagt gccattgtgt ttgcttgcgc gacactaaac
     5581 tccatcttct taacgttaga gctatgagaa tccctatgcg gttttcaaac ccgtcactgc
     5641 agaatggccg ttgcaatgcg atcggccgga ctcccccaaa cgataccccc tttgctaaac
     5701 cctccgggcg cttgggccac gttcgtgggc atatcgaaat atcgggccat cgctccttgc
     5761 ccggccttca caagcgcgtt gcgtcgctca tcggtcatgt cgaactcggt cgtaccatag
     5821 ccctgcgccg gaagtcgtac gacaagatga ctgtactcat ccatcaccat cttgtcatgc
     5881 gcaccggttg ccgtatcgac aagccgccgt aaccgctgta cggtttggag ttcgcccggc
     5941 ttaatcgtaa tattcacgaa aattcccttc gctaacgaga ccgggagctt ttcgtcgatg
     6001 aggagaccta ggaccggcgt gtcctgcttc ggtcccatga gcttcgtgac ttgtagagca
     6061 tcggagatga agagttcgat cgggaagttc gagaggatgc ccccgtcgac gatggcatgc
     6121 ccctccatat cacgaccgag atattgtccc cagttggctt tccaaatgat ctcatcccac
     6181 acgagcggga tgctcatcga catccgcacg gcccagacta tagggcactt tggtgccgtt
     6241 cggtggttca gaacgagcag gctttcatcg gtagtatcag aggcgactag cgataactcg
     6301 atgttcgtgg ccgcatagaa ctgactgaga gtcatcttac tgaactgacg agggcctact
     6361 gtcgtgcttc ccgaatctaa cttcgaactc aaccaggtaa caaatcgatc tgcagcgaac
     6421 caaccgccac gttcaatgag agcaagaata tggcggaacg cccctccatt ggccatggca
     6481 tcaagaatca tttcgtcaat ctttttctcg atgaaatcgg ggatgacggt aaaatcgact
     6541 ccctcgagca atcgcttagt cgcactcccg cgtaattctg ctttactgaa tgatgcagga
     6601 ggacccatga aactcgtaaa gaccgattta ccttcggggt ctttttcgac caatgcggcg
     6661 agcatttctt ccggcgtata gccggcggcc aatagcgtgg cagtgatcgc cccagccgag
     6721 gtgcccagca atcgattaaa tgtgtggcct cgccggaaga attcctcgca ggcacccaca
     6781 aacaccattc cttttgctcc accgccttca aacactagat cgtatttcac catttgcctc
     6841 cttattaatg gttggttagc gggactgcga agcagtcggg accagaacgc gcaagccgat
     6901 cagcaggcac ttgcaatcac actatcgcgc accttttcca ccaattcact tgccttggct
     6961 ggcttgaacg atatcctctc tccctgctct agaccgcaat atcgagcggt tgctttgttc
     7021 aggacttatt gatcagacgc gttgcctcat ctcaaagggc gatacgctgc tgatcgagtt
     7081 tttatgttga agcggatcga ttgtccccgt catcagaccg atcagattgt tgataacctc
     7141 catcaaccct ggtccattgg gcgggatgga ctcatacctt tatttgactc cgaagccttc
     7201 ctaggatgcg tgctgaacac taatactaga gaacatagtg cgtccttgag ataggagcga
     7261 tactaagtaa ttctgtgcaa ccttatactc ctaaaatgca tataggtgct agcgggaagt
     7321 ttcgagatac cactatgaat ggaagcattt atgagatgcc tcctttcgcc acaaggctcc
     7381 attcaataga gtcccagcct ttccccacct gccacctatt tcagactcgt tcccaaatcg
     7441 atcaagtggg acttctgttt tcttggcttg ccaaaccatc accggtcaac actctagaga
     7501 atttgacaga aatgatggaa cagactcttt gttgtctgtg gaattattgg aacaggatcg
     7561 cggcgttgca tagctcttag ctaacgattc tttcatgcaa gatttggaca atatttctct
     7621 accaccaagt ggagagtgcc cttacaccac acatcggcta ttcctactaa gaccgtgagc
     7681 ttgcaaatta gctggttttt cattatgaga cgggtatcaa gtgagtagat atacgctttg
     7741 cgcgtttcag ccattaattc tagaaactcc attacataag cgtggccatc tggcttgata
     7801 taacgccgga tcttttaaag tggtaattca agtccctaaa aaagtggtcg acaatattga
     7861 tcagtttcat ggtcgtgaat ggctgctccc gaaggtcttg gagtggtggc acaagggtaa
     7921 ggaccgccta tttctcttga ctggtgagcc tgggaccggc aaaagcatga ttattggatg
     7981 gcttggcggg ttcggaaatc caccaattaa tgtgactccc aaggcggaat tagcccaggt
     8041 tcgcaccttg ataaaagcag tgcacttttg tcaggctgca agtcgcaatg ttacacccca
     8101 agcatttgca gacagcatgg ccc
//
LOCUS       DAOQEW010000175         8109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3055_length_8109_cov_7.474795, whole genome shotgun sequence.
ACCESSION   DAOQEW010000175 DAOQEW010000000
VERSION     DAOQEW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8109
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3055_length_8109_cov_7.474795"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLS35_15550"
     CDS             <1..653
                     /locus_tag="PLS35_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNI20138.1"
                     /translation="MTPEREEELRSYFHKLALPYNQHLGAVWMRLGDDGIPYVAFPLP
                     TPNIHFMQGLMARALGEAPPALMRVARGLKGPWILKRGNAPILITESIVDCLAGDELF
                     GPSFTLCALNGLNTVERLQEYLKRLPSRIIYVALDNDASCIAVGNAPQNSSRKKGPHV
                     QKELVSLLTHMGYHVMEVLLHHNASVKDLHKLWLKHPQRVSLLDLAKTGLHHAPAC"
     gene            877..2451
                     /locus_tag="PLS35_15555"
     CDS             877..2451
                     /locus_tag="PLS35_15555"
                     /inference="COORDINATES: protein motif:HMM:NF025085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HNI20139.1"
                     /translation="MFSEDFYPTPGAVAAKMLQKIDRNAVHFLEPSAGKGDLAKAILG
                     FGRTRSPYDHGSRHRVDVIELHPDLLKILQAHEELTVVGYDWLTYDGVSYYDAIVMNP
                     PFSKGALHLLRAWDFLHNGEIVCLLNQETIDNPYTEDRQRLAAIIAAHGSVEPLGPCF
                     LTAERPTDTQVALVYLKKTTEDDRIHLWHSADREQSVNDDIGTPEAIPALRDTLGNMQ
                     HYYTQSLEEMFKAFAHIRKASLFMGALGTELRPSRSTRDESDLKKILGLAQTNTTAAR
                     AEFARALRRSAWMHVFEQMDFRKWLDTKQTEELLQDLERDSTVPFTTQNIKGTLSNIF
                     QQRKRLFEKSVWNVFLALTRHYKGNTTGDIGSGDGKAGWKSNDSYKVNCRLVFPYGCR
                     FWIGRFDLWSARDAGEIYSDLDRVLAVLDGQSFEEIVTVRDALERSFHNHGVHPHPCE
                     STFFRIRYFKKGTVHLKWKREDLLATFNTTAAAGRQWIGTTCHRDQDSQASYSHLDSN
                     DPHSWPSPVPTCQICA"
     gene            2526..2774
                     /locus_tag="PLS35_15560"
     CDS             2526..2774
                     /locus_tag="PLS35_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20140.1"
                     /translation="MLEQHNKRWAIKGTTTKTRVDLEPIVEVTDRANEREEGTTERTI
                     ERFTPVIQGWDLTPGESFGQHLIIDCEAPPPKPESKAR"
     gene            complement(2775..3494)
                     /locus_tag="PLS35_15565"
     CDS             complement(2775..3494)
                     /locus_tag="PLS35_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20141.1"
                     /translation="MTTSVNSPINSPVKTISVVFADDHALVADGMTTILHGVNHKQRD
                     YEFAVVGQASSGRGLLELVKRVPADLAFVDISMPDMSGLDAVPKVREIRPDIKIIVVT
                     MHKEPEYMTRALSVGANGYLMKSCSAQEFCHAIDEVMRGNAYVTSLLGSRPTGALPGS
                     DDEEDQLASLGMSQRQREVVTLLASGKTIKETAFQLGLSRKTVEHHKAMFREKFGIET
                     DADLTKFAIKHGLTGTDWIQK"
     gene            complement(3491..5143)
                     /locus_tag="PLS35_15570"
     CDS             complement(3491..5143)
                     /locus_tag="PLS35_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI20142.1"
                     /translation="MLSGNICLACSTGDEQYWSAKAKSGEGKPYSREVGFAFAEEIYR
                     RLLKLNESLVVDQEKKYFEDVQELHQTDLKMRRIADAVPGAVYQYVITRDGCQRFSYI
                     SRGAMDMVGYPSDVIVSDYSAVWKLVLPEDMPGIMASIENAIRSGSRWAHEFRLRLPD
                     GRVKWLRGDSLPERPTVDGTVLFHGLLTDVTERRLAEAKLRFTQFATDHAADAILWAG
                     PDRRIMYVNVRATELLGYSREELLNLSMPDIAPLQSRIDILNDLTRLKQGEVVQYATT
                     FRRKSGAVFPIEVSMRYLEHDGEGYVCAIVRDVSERKRAESVIQEGAEALRRSQEALR
                     ALHSQLLTAQEDERRRVARELHDDLGSRLGSVILRAEGKLVLLENKSPEAAVIKNVTD
                     ELRGISDRVRTIAHELHPSILDNLGLSVALKKMLKEYGSWSGMKIVSNISMEFTEALD
                     REVATCLYRIAQEALMNVKKHAGAQQVMVSLAREEGWVQLSVKDDGMGFSPETESRRT
                     KGLGIVAMEERIHMVAGELAVQSGKGRGAVVVARAPYREREA"
     gene            6213..7028
                     /gene="traT"
                     /locus_tag="PLS35_15575"
     CDS             6213..7028
                     /gene="traT"
                     /locus_tag="PLS35_15575"
                     /inference="COORDINATES: protein motif:HMM:NF017619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HNI20143.1"
                     /translation="MSRINRRAHCSVGRTLAILSVVIATGSIVGCAGHMTLSHEVNLT
                     SSNAIFLTPSEEKTIYLQNRNTSDNPNINFAELPNKIRAKGYAIVDNPDKAQYILQSQ
                     VVLCNKLKPGQTVDALIAGGFGGAIGVAAGSAAALSGASLSQIPILGAVGAVGGFAAS
                     KFSEDSIVACVVDAMVQERTKEEIQQSVTTNSMPGPGTPQSQPGAFGFLNQQSVSPPQ
                     AGQVTQQISEIRKGTRRFHQARIVASDQKMWLSVPEASKVLSDKLEDSLAGLF"
     gene            complement(7397..7753)
                     /locus_tag="PLS35_15580"
     CDS             complement(7397..7753)
                     /locus_tag="PLS35_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20144.1"
                     /translation="MAIKRLIGRGISVVLGVMILHVFMGCQGEELAEIDGDVNVLEHP
                     LPLRFSDPKTYTHPPDSLPINKVLVVLTKGQMVPVMRRIYGKEFLAYEIQLPDSSKGY
                     LFHGDAFHLVDSRRAP"
     gene            complement(7765..>8109)
                     /locus_tag="PLS35_15585"
     CDS             complement(7765..>8109)
                     /locus_tag="PLS35_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20145.1"
                     /translation="INGTTYSFGPNGMTNEPTSVFNQRNSFRSSLGLDLTLTAEQEKA
                     LEECLKRDQPDYNFLTNNCGAPVQRCLNWMGYDIGKVLTPSQLYESMTGVGLVGGTIF
                     YPGAGTKPAQAK"
BASE COUNT         2014 a         2441 c         1890 g         1764 t
ORIGIN      
        1 acatgacccc ggaacgtgag gaagagctcc ggtcgtattt ccataaattg gcgctcccct
       61 acaaccagca tcttggcgct gtatggatgc ggctaggcga cgacggcatc ccatacgtag
      121 ccttcccgct tcccacaccc aacatccatt tcatgcaagg gctgatggca cgagccttgg
      181 gcgaggcccc acccgccctg atgcgggtcg cccgcggcct caagggcccc tggatcctta
      241 aacgcgggaa cgcgccaatt ctgatcacgg aaagcatcgt ggattgcctt gcaggcgatg
      301 agctcttcgg accctcgttt accctctgtg ccctgaatgg gctgaatacg gttgagcgcc
      361 tccaggaata tctcaaacgg ctcccctcac gaatcatcta tgtcgcgtta gataacgacg
      421 caagctgtat cgccgtggga aatgcgcctc agaacagctc acgaaagaag gggccacacg
      481 tccagaaaga actggtctcg ctgctgactc acatgggcta tcacgttatg gaagtgctac
      541 tccatcacaa cgccagcgta aaggatcttc acaagctttg gctgaagcat ccgcagcgag
      601 tgtcgctgct cgatttggca aaaacgggcc tccaccatgc accagcgtgc tagcgcagcc
      661 gcgtcacatt ccatggcctc gtccgtgacc tctgacaccc cgtcacactc acggtatgac
      721 acccactact acgaccacag gagctgaatg accacggcgt gacctacctg gtagacactg
      781 tgtcgatgaa gccctagata gggccgcact ccggaatccc caacagcgtc aacctattca
      841 agtcacagtt gcctgcgtct gtcgaaaagg agcggtatgt tcagcgaaga tttctacccc
      901 acgcccgggg ccgtcgcagc gaaaatgctt cagaaaattg atcgcaatgc tgtccatttt
      961 cttgagccga gtgccggcaa aggcgatctc gctaaagcca tattgggatt cggtcggact
     1021 cggtctccct acgatcacgg ttcacgccat cgcgtcgacg tgatcgaact ccacccagat
     1081 ctcttgaaga tcctccaagc ccatgaggag ctaaccgtcg tcggatacga ttggttgacc
     1141 tacgacggcg tctcctatta cgacgccatt gtgatgaatc ctcccttcag caaaggtgcc
     1201 ctccatctac tccgcgcttg ggattttctg cacaacggcg aaatcgtctg cctcctgaat
     1261 caagagacca tcgataatcc ttacacggaa gaccgtcaac ggctggccgc gattattgct
     1321 gcacatggct cggtcgaacc tcttggcccc tgcttcctca cggcggaacg gcccaccgat
     1381 actcaggtcg ccctcgtcta ccttaagaag actacggaag atgatcgcat ccatctttgg
     1441 cattcggccg accgcgaaca atctgttaac gatgacatcg gcacacctga agcgatcccc
     1501 gcgcttcgcg atacccttgg aaatatgcag cactactaca cacaatccct agaagagatg
     1561 tttaaggcct tcgcccacat ccgcaaagcc agtctcttta tgggcgcact cggcacggag
     1621 cttcgcccga gccgatcgac gcgagacgag tccgatctta aaaagatcct tggcttggcc
     1681 caaaccaaca ccaccgccgc gcgcgccgaa ttcgcacggg cccttcgccg aagcgcctgg
     1741 atgcacgtct ttgaacaaat ggacttccgg aagtggctag atacgaaaca gacagaagaa
     1801 ctcttacaag accttgaacg cgatagcacc gtgcccttta ccacacagaa catcaaaggc
     1861 accctctcca atatattcca acagcgcaaa cgcttgtttg agaaatcggt atggaatgtg
     1921 ttcctcgcgc tcacacgaca ctacaagggc aacaccaccg gagacatcgg gagcggggac
     1981 ggcaaggcgg gctggaagag caacgactcg tacaaggtca attgccggct tgtgtttcca
     2041 tacggctgtc gattttggat cggacgattc gatctctggt ctgcccgtga cgccggcgag
     2101 atttactccg atctggatcg cgtcctcgcc gtgctagatg gacaatcatt cgaagagatt
     2161 gtcacggtgc gagacgcgct ggaacgctca tttcacaatc atggggtgca cccccacccc
     2221 tgtgagagta ccttttttcg tatacgctat ttcaaaaagg gaaccgtgca tctcaaatgg
     2281 aaacgagagg acctgctggc caccttcaac acgaccgcag ccgcaggacg tcagtggatt
     2341 gggaccactt gccatagaga tcaggactcc caagcttcat attctcatct agacagtaac
     2401 gacccccaca gctggccatc gcccgtaccg acatgccaga tttgcgccta gagagtagtc
     2461 ccagactcca agcctaaagg ccgatcggcc ttggatatgt tgccagcggt ctcgtcaata
     2521 acaccatgct tgaacagcac aacaagcgct gggcaatcaa gggcaccaca acaaagacac
     2581 gcgtcgatct cgaaccgatc gtcgaagtca cagacagagc aaatgaacgg gaggaaggaa
     2641 caacggagag gacaatagag aggtttacac ccgtaatcca gggatgggac ctcaccccgg
     2701 gtgagagctt cggccagcac ctcatcatcg actgcgaagc gccaccaccc aagcccgaaa
     2761 gcaaagccag gtagttactt ctgaatccaa tcggtcccgg tcagaccatg cttgatcgcg
     2821 aacttggtta ggtcggcatc tgtctcaatg ccaaatttct ccctgaacat tgccttgtgg
     2881 tgttcaacgg tctttcgact cagacccaat tgaaacgcag tctctttgat cgtcttgcct
     2941 gacgccagca acgtcacgac ttcacgctgc cgctgcgaca tccccaagga ggcgagttga
     3001 tcctcttcgt catcagaacc aggcagagcc cctgtcggac gactaccaag caggctcgtc
     3061 acatacgcgt tgcctctcat cacttcatcg atcgcatggc agaactcctg agcactgcaa
     3121 ctcttcatca gatacccatt cgcccccaca gataacgctc gagtcatgta ttcaggttct
     3181 ttgtgcatgg tcacgacgat gatcttaatg tcaggcctga tttctcggac cttcggcact
     3241 gcatcaaggc cagacatatc cggcatcgag atatcgacaa atgccaaatc cgcaggaacc
     3301 cgcttcacaa gttcgagcaa tccacggcct gaagaggctt ggcccaccac ggcgaattca
     3361 tagtcacgct gcttatggtt aactccatgt aagatggtag tcatcccatc cgcgaccaac
     3421 gcatggtcgt cagcaaagac cacgctaatc gtttttaccg gagagttgat cggagaattc
     3481 acactggtcg tcatgcttca cgctccctat aaggtgcccg agccactacg acagctcctc
     3541 gccctttccc tgactgaacc gccaattccc ctgccaccat gtggatgcgc tcttccatcg
     3601 cgacaatccc tagtcccttg gttcgacgcg attctgtctc tggactgaac cccattccat
     3661 catctttcac actcagttgc acccaaccct cttctcgagc caaggatacc atgacctgtt
     3721 gcgcaccagc gtgtttcttc acgttcatca aggcttcctg agcaatccga taaagacagg
     3781 tcgcgacctc ccgatccaag gcctcagtaa actccatcga tatattactg acgatcttca
     3841 tccccgacca actgccatac tccttcaaca ttttcttgag cgccaccgag agcccaagat
     3901 tatccaagat cgaggggtgc aactcgtgcg caatcgttcg cacccgatca ctgattccac
     3961 gtagctcgtc agtgacattt ttgatgaccg ccgcctcagg gctcttgttc tccaacaaca
     4021 ccaatttccc ttcggccctc aaaatcacac ttcccaatcg cgatcccaga tcatcatgca
     4081 actccctggc aaccctgcgt cgttcgtcct cttgcgcggt cagcaactgt gaatggaggg
     4141 ccctcaacgc ttcttggctg cgacgcaggg cctcagctcc ctcctgaata acactttcgg
     4201 cacgctttcg ctctgacacg tctcgcacga tggcacacac atacccttcc ccatcatgct
     4261 ccaagtagcg catcgacact tcgatgggaa agaccgctcc agactttctg cggaatgtag
     4321 tcgcatactg aaccacttcg ccctgtttca gccgcgtcag atcgttaaga atgtcgattc
     4381 ggctctgtaa aggagcaata tccggcatcg acaaattcaa gagttcttcg cgtgaatagc
     4441 ccaacaactc ggttgcccgt acattcacat acatgatgcg acgatcaggc ccagcccaaa
     4501 gaatggcatc agccgcatgg tccgtcgcaa actgcgtaaa ccgcagcttg gcttcagcca
     4561 accgtcgctc agtcacatcg gttaacaaac cgtgaaacag tactgttccg tctaccgtag
     4621 gcctctcagg cagagaatcc ccacgcaacc atttcacgcg accatcgggg agtcgcaacc
     4681 taaactcatg cgcccaccgc gatccactgc ggattgcgtt ttcaatggat gccatgatcc
     4741 ccggcatatc ttctggtagg accaatttcc agaccgcact atagtctgac acaatgacgt
     4801 ctgatggata ccccaccata tccatggctc cacgactgat atagctaaac cgctgacatc
     4861 catcccgcgt aataacatat tgatacaccg caccgggcac agcatccgca atccgtcgca
     4921 tcttgaggtc agtctggtgt aactcttgga catcctcgaa atacttcttc tcctgatcga
     4981 ccacgagcga ctcattcagc ttcaagagcc ggcgataaat ctcttcagca aatgcgaatc
     5041 caacctctct ggagtacggc ttcccttcac cactcttagc cttagcactc caatactgct
     5101 cgtccccagt agaacacgcc agacaaatat tgcctgataa cacttgcaac aacgatctga
     5161 tcctagaatc gactgtcacc ctcgagtaga ggaagacaca gtagctccct ccagaaggca
     5221 gcagcccacc caggcaaata acctgtttca ctccgtgcct ccgcagaaag tctgctctgc
     5281 tttcggcgag gttataagag cgtgggatct catgcaccag gattccaaac aattgattgt
     5341 gtgaattagc gacagtaaga ctcttctcaa aattattcac cggactgctc agccccaaat
     5401 cctcccacga ttgcgtgtac cacgcagttt cctgggaact gaacttcgtt tgaatgggga
     5461 cacaatgtag tccgaacgga ggtctgtcga tcgtccactc cgcagaatct ccggccacgc
     5521 ccatcaatgc aaggactcgc gtttgagcgg gggcgtggtc cccatcaacc gaagacaccc
     5581 atcggtcttg gtagcgcgct gggagatcac caagattacg agtgtgaaag acctcgacga
     5641 gagacaattg agactgacga ctctcctggg aacagaaaga ccgggccaga gacatcgcaa
     5701 tgagttgaca gacctcctcg gaagattttc caactgtctc taaatctcgt acccacgagc
     5761 ctaaacgaca cgcatcaccg agggagagct ctcggagatc aaacgtcgat tcttggcctt
     5821 tgacgctcaa cgacatacta ccctccaact ccctctcacc aactcacaac tcagtaggcc
     5881 gctctccgat tcactctgcc gtcagagaat cgctcgtgga cgtcacacca actctatgca
     5941 cattacacgt attacgacta ctcgtcccag gcacaggcgg cagtatgaca aaaacacgct
     6001 ctgtgactca atacccagct ttcctgcgca tggttttttc catacacaca ctcctttcgg
     6061 tctgattgtt tcaaattggc acatggcaac tagttcggtc tcatccactc tgtgacgacg
     6121 acgcaaacgg gtatcgccaa ggaccatcca ctgccatcca gtaatacata gcagaagcgg
     6181 ttcacactcg ttcacatacc caggaggaag gcatgtcacg tattaaccgt cgcgcccact
     6241 gttcagtcgg cagaactctc gccatcctta gtgtcgttat cgccacagga tcgatcgttg
     6301 ggtgtgctgg ccacatgacg ctaagtcatg aggtgaatct cacaagcagt aacgccatct
     6361 ttctcacccc gtcggaagag aagacaatct atctccaaaa tcgcaacacc agcgacaacc
     6421 ccaatatcaa ctttgctgaa ctcccgaaca agatcagagc aaagggatat gcaatcgttg
     6481 ataatcccga caaagctcag tacatcctcc agagccaggt agtactctgc aataaattaa
     6541 agcccgggca aaccgtcgat gccttgatcg ccggcgggtt cggtggggcc atcggagtcg
     6601 cagctggctc ggccgcagca ctcagcgggg cgtcgcttag ccagattcca attctcgggg
     6661 cggtaggagc ggttggggga tttgccgcta gcaaattctc agaggactct atcgtcgcct
     6721 gcgtggtcga cgcgatggta caggagcgca ccaaggaaga aattcaacaa tctgtcacga
     6781 ccaatagtat gccagggcca ggcacaccac agtcacaacc tggagctttt ggctttctga
     6841 atcaacaaag tgtctccccg cctcaagctg gacaggtcac ccaacagatc agcgaaatcc
     6901 gaaaaggcac tcgccgcttc caccaagcga gaatcgtggc gtctgaccaa aagatgtggc
     6961 tgtccgttcc ggaagccagc aaggtactca gtgacaaact tgaggattcc ttagctggac
     7021 ttttctaaag aatgactgac cctgataggg cgcgtacgtc gtggaatcaa gagtcacgaa
     7081 cagggtgacc atcgggcacg attaaaatgg tgagcatgtg gatgaaggcc atcatcacta
     7141 gctgagattg cctaagagac aagtgcctga gcgtcgggca gccacgtatg gctcgcaatg
     7201 atcagccgat ccaattgatc atagccaaac gtctgcgacc ctcggcgatc cgtcagactc
     7261 gttcgattgc cgactgggtt gtagacatag tccgccttgt tgatctggct gctggtcgcg
     7321 gtgagttgag caggatgctg gtgacctgtg aggcgggata ataggtgtag cccatatcag
     7381 gctcgaacag ttgcagttat ggagcgcgtc tcgaatcaac aagatgaaag gcatcaccat
     7441 ggaacagata tcctttacta ctgtcaggca attggatctc atatgccaga aactctttcc
     7501 catagatacg tcgcatgact ggaaccatct gccctttcgt gagcacaact agcactttat
     7561 tgatgggaag tgaatctggc ggatgagtat atgtcttggg atcagaaaac cttagaggca
     7621 acggatgctc tagcacattg acatcaccat cgatttcggc cagttcctca ccttggcacc
     7681 ccatgaagac atggagaatc atcaccccta gaacaaccga aatgccccga ccgatcaacc
     7741 tttttatcgc catagcataa acctctattt agcttgagca ggcttggtac ccgctccggg
     7801 ataaaagatc gtaccgccta cgagaccaac tccagtcata ctttcgtaaa gctgtgatgg
     7861 tgttaatact ttaccaatgt cgtaacccat ccaattcagg catctctgca ctggcgcacc
     7921 gcagttgttc gtcagaaagt tatagtcggg ttggtcccgt ttgagacatt cttccagtgc
     7981 cttttcctgt tcggctgtga gggtcaaatc caaccccagc gaactccgga acgagttcct
     8041 ttgattaaat acggaagtgg gctcgttcgt catcccattt ggcccgaatg aataggttgt
     8101 gccattgat
//
LOCUS       DAOQEW010000176         7931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3164_length_7931_cov_6.325038, whole genome shotgun sequence.
ACCESSION   DAOQEW010000176 DAOQEW010000000
VERSION     DAOQEW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_7931_cov_6.325038"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3028)
                     /locus_tag="PLS35_15590"
     CDS             complement(<1..3028)
                     /locus_tag="PLS35_15590"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HNI20146.1"
                     /translation="MRSTYQPIRRLLVALAALLLLDPGHGLDAKETDTETPIGAVGGS
                     IHGDPFTGTATASIPIEIPPGRNGVQPGLQLVYSHSNGNGWIGMGWKLELGSIERQSR
                     FGVDYAKNDYTIQMSGMSTELVQAPLPASSDEYRAKFEGSFSKIKMLSNGGFEVTDKK
                     GTKYLFGQSGVSRVSHPTDSSKIYKWLLDRVEDRDGNFMTATYSADQGQEYLSQITYT
                     GNGAAQPGNTIKFYLESRPDGQTLYTANFQVITAQRLKTIEVLSGTTRLRAYKLAYST
                     SASTGRSVLTSVQQFGKDAVLDGSFTVTGGTSLPPVSMTYENESMSLGSDQLWGSKSY
                     GIGCAAASNCRPNFAYVDMNADGRTDFIYQRSDIDEMHVLLSTGSGFGSDTNWGMKSF
                     GIACAGASSCQPAFSYADMNGDGKTDFVYMRSDLDQFRVMLSTGTAFSTDTVWGSKSF
                     GIACAGASNCRPNFAFTDVNGDGKADFVYQRSDIDELRVLLSTGIGFSADTNWGMKSY
                     GIACAGASNCQPNFAFTDLNGDGKADFVYQRSDIDELRVMLSTGTAFGPDTSWGVKAY
                     GIACAGASNCIPNFTFADINGDGKADFVYQRSDIDELRVMLSTGTGFATDTAWGMKAY
                     GIACAGASNCRPAFGYFDVNGDGKADFVYQRSDIDELRILLSTGSAFRPDAVWGMKGF
                     GIACAGAMNCQPSFSYVDTNGDGKGEFAYHRDATDQIRVLPSSPDGILLATMTNGTGG
                     AAITTYAPSTQFSNTLLPYTTRLLTSTVTCDNWGGSSCVGLSSTTTYTYSGGYHHLAE
                     RDFRGFNYAKVTGPVGPTGEQLITETWFHQGNDVAVDTNNPNVANGYTKGMPYRSKVT
                     DATGKVYSETTTTYIADVDGAAPFFTPVAQVDASIENGAKQTRSVFAQYDAYGNVLRE
                     DQYGDLATTADDRTVVRTFANNTTDWLLGFPTSETSYAGIGTTTQVAQTNLYYDGTTS
                     CATASVNQVPTKGHVTRTVRWLNGGTSPETRMAYDAF"
     gene            3661..4086
                     /locus_tag="PLS35_15595"
     CDS             3661..4086
                     /locus_tag="PLS35_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI20147.1"
                     /translation="MDWQAVRVQQMVSSPQGGVLLRGFGVPDELPHECGRLLLLIEPI
                     IEQGEADSFSSREQIRLTHREGAVARYLAAGLTNKEIANHLHLSEYTVKDHVKRLMRK
                     TQTTTRTATVSRLLLQYPSLSKAKVTDGIMGTIFAKQAV"
     gene            complement(4628..5845)
                     /gene="tyrS"
                     /locus_tag="PLS35_15600"
     CDS             complement(4628..5845)
                     /gene="tyrS"
                     /locus_tag="PLS35_15600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNI20148.1"
                     /translation="MTPLAQQLDLILRGIVEVIQQSELESKLARSIKENRPLRVKAGF
                     DPTAPDLHLGHTVLIHKLKHFQDLGHQVIFLIGDFTGMIGDPTGRSETRVALSKEKVL
                     ENAKTYERQIFKILDPAKTQVEFNSRWMSTMTADGLIELSAHYTVARMLEREDFHKRY
                     TEGTPISIHEFMYPLIQGYDSVALKADIELGGTDQKFNLLMGRDLQRDYGQEAQAVIT
                     MPLLEGTDGVRKMSKSFGNYIALEDQPADMFGKLMSISDTLMHRYYELLTTENLAGVK
                     AAHPMDAKQGLAEQIVARYHGADAGREARTAFQQKFQAREFPEQPDAHVVLTPSDVKD
                     ASAPSIGLVDLIAKTGLVPSKSEARRLIVQGGVEINEQKMTDANGAVPLLVGQLLRLR
                     VGRRKFAVAEYKD"
     gene            complement(5842..6390)
                     /locus_tag="PLS35_15605"
     CDS             complement(5842..6390)
                     /locus_tag="PLS35_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNI20149.1"
                     /translation="MMQSLLIKVAMLAVTLGSLVWIASVTPVTKVMPGQPQLLPKHSV
                     TTVSSPPPVEVPATRPALASVSQAGTPSRRQVAMPIEESESASARVSEKASTDSPSAR
                     QAGARQVDLNQATLSELEALPGIGPKLAQRVIEHRDENGPFRSVDDLRQVKGIGRKKF
                     DRLRPHVLVTNTKSLARHKGTL"
     gene            complement(6387..7814)
                     /gene="lpdA"
                     /locus_tag="PLS35_15610"
     CDS             complement(6387..7814)
                     /gene="lpdA"
                     /locus_tag="PLS35_15610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNI20150.1"
                     /translation="MSKHLAILGAGPGGYVAAIRAAQLGARVTVVENQALGGVCLNWG
                     CIPSKALLSVVELGDKAKKAKDFGLLLNGPVAYDPAIMVARKNKVVSTLVKGIATLFK
                     TWNIEHVEGTGELVDARTIRVTKPDGTTLQVAADGIIVATGSSWPNLPLFPIDGTQII
                     TSKQALDLTRIPASLLIVGGGVEGCEFASLYSGLGAQVTLVELVPRILPLEDEEISQM
                     MERELKKRGVDIRTGVTVDQIVRQSDVVTAHLRDGLSLNVEQVLVSVGRGFNSRGIGL
                     EKAGVQVGSRGEIVVNERMETNVPGVYAIGDVVGKAMLAHVASQQGKVAVENFMGHAR
                     AIDYDVIPTGIFTLPEIGRVGLTEQQARDRCVGAGKDPQQSVKVGRFRYGGLGKAQAT
                     GDIQGLVKVVVDTESDRILGVHILGAHATDLIHEAALAMHLGATVARAAEMIHAHPTL
                     AEGLMEAMEDVHGHAIHLARKPATT"
BASE COUNT         1776 a         2321 c         2055 g         1779 t
ORIGIN      
        1 cgaaggcatc gtaggccatc ctggtttcgg gactggtgcc gccgttaagc cagcgtaccg
       61 tccgggtcac atgccccttg gtcggcactt gattcaccga cgcggtggca cagctcgtcg
      121 tgccgtcgta ataaagattg gtttgcgcca cttgcgtggt cgtgccgatg cccgcatagc
      181 tagtctcact cgtcggaaac ccaagcaacc agtcggtcgt gttgttggca aaagtccgca
      241 ccacggtccg atcatcggca gtcgtggcca gatccccata ctggtcctcg cgcaacacgt
      301 tgccgtaggc gtcgtattgg gcgaagacgc tccgggtttg cttggcacca ttctcgatcg
      361 atgcgtctac ttgcgccacg ggagtaaaga aaggggcggc gccatccacg tcggcgatat
      421 aggtagtagt agtctcgcta tagaccttac cggtcgcatc ggtgaccttg ctacgatagg
      481 gcatgccctt cgtgtacccg ttcgcgacgt tggggttgtt ggtatcaacc gccacgtcgt
      541 tgccctgatg gaaccacgtc tcggtgatca gttgttcgcc ggtcggtcct accggccccg
      601 tcaccttggc gtagttgaac ccacggaaat cacgttccgc gagatggtga tagccgccgc
      661 tatacgtata ggtcgtcgtc gaggacagtc ccacacagct actcccgccc cagttgtcgc
      721 acgtgacagt ggaggtaagc agccgcgtag tgtatggtag gagggtgtta ctgaattgag
      781 ttgatggtgc gtatgttgtg atagcagccc cacccgtacc attggtcatg gtggctaaca
      841 ggattccatc gggagaactt ggcaatacac gaatctggtc tgttgcatcg cgatgatacg
      901 caaactcccc ttttccgtca ccatttgtat caacataact aaagctcggc tgacagttca
      961 ttgcgcccgc acaggcgatc ccgaagcctt tcattcccca tactgcatcc ggcctaaaag
     1021 cactgcccgt ggacaggaga atccgaagct catcaatgtc ggagcgctga tacacaaagt
     1081 ccgcttttcc atccccattc acatcgaaat acccaaaggc aggtcggcag tttgaggcgc
     1141 cagcgcatgc gatgccgtaa gctttcatcc cccatgcagt atcagtcgcg aagccggtcc
     1201 cagtggataa catcacccga agttcatcta tatcggatcg ctgatagaca aaatcagcct
     1261 tgccgtcgcc attgatatcc gcaaatgtga aattgggaat acaattggac gcccccgcgc
     1321 aggcaatacc gtaggccttc acaccccaac ttgtatcagg cccgaaagcc gtccccgtcg
     1381 acagcatgac tcgcagctcg tcgatatccg agcgctgata aacgaaatcg gccttgccgt
     1441 caccgttcag gtcggtgaag gcaaaattcg gctggcaatt cgacgcgcca gcacaggcaa
     1501 tcccataact cttcattccc caattcgtat cggcactaaa tccaattccc gttgaaagga
     1561 gtactcgcag ctcatcaatg tcggatcgct gatagacaaa atcggccttg ccgtcaccat
     1621 tcacatcggt aaaagcgaaa ttgggcctac aattcgaagc tcccgcgcac gcaattccaa
     1681 aggatttgga gccccatacg gtatcggtgc taaatgcagt tccagtcgaa agcataactc
     1741 ggaactggtc taggtcagat cgcatataaa caaaatcagt ctttccgtcg ccattcatgt
     1801 cggcatacga aaacgcaggc tgacaactcg atgctccagc acacgcaata ccaaaagact
     1861 tcattcccca gttcgtatcg cttccgaatc cggaaccagt agaaagcaac acgtgcattt
     1921 cgtcgatatc ggagcgttga tagatgaaat ccgtacgccc atccgcgttc atatcgacat
     1981 aggcaaaatt ggggcggcaa ttcgatgcag cggcacagcc gatgccatag gacttcgagc
     2041 cccacagctg atcggatcca agcgacatac tttcgttctc ataagtcatt gacactggtg
     2101 ggagggatgt tcccccggtc acagtaaaag aaccgtccag caccgcgtct ttgccgaact
     2161 gttgaactga agtcagtaca gaccggcctg tactggcgct cgttgagtac gcgagtttgt
     2221 aggcccggag tctggttgtg cctgacaata cctcaatcgt cttgaggcgt tgtgctgtaa
     2281 taacttggaa attcgcagta tagagagttt gaccgtccgg tctgctctcc aaatagaact
     2341 tgatggtatt acctggctgg gctgctccat ttcccgtata ggttatttgg gacagatatt
     2401 cctgcccctg atcagcgctg tacgttgcag tcatgaaatt gccgtcccga tcctccacac
     2461 gatcgagaag ccacttatag atttttgatg aatcggtggg gtgagacacg cgagaaacac
     2521 cagattggcc gaataggtac tttgtccctt ttttgtcggt gacctcgaag cctccgttcg
     2581 agagcatctt gattttggaa aagctaccct caaacttcgc tcgatactca tcgctggagg
     2641 ctggaagcgg agcttgtact aactcggtcg acataccgct catctgaatt gtgtaatcgt
     2701 tcttcgcgta gtctactcca aaacggctct gccgctcgat cgaacctagc tccagcttcc
     2761 atcccatccc aatccaaccg ttcccattgc tgtggctata cacgagttgc aagccaggtt
     2821 gaacgccatt tcgtcccggc gggatctcta tgggaatact cgcggttgca gtgcctgtaa
     2881 acgggtcgcc atgaatactc ccacccactg caccaattgg ggtctccgta tccgtctcct
     2941 tagcatcgag gccgtgtccg ggatcaagca agaggagggc agccaaggca accaggagtc
     3001 gacgaatcgg ctgataggtg gaacgcatcg ctgaagctcc ttgtgacgcg gtggtggatt
     3061 tctgttgtta ctcacgcacg cggcggagca tacgggttgc cacctggtcc ccacaatcac
     3121 cccagatggg ggtacgccta ttggataaca gtagttacac ggtaggtcct cacaatctga
     3181 attgccgaga actctgcgcc agccatcgga gctaagaaac gctcagggga tacaatcacc
     3241 gtttgtatgg cgtggcctcg gacagctcac atccagaaca gttgagtctt gattgccagg
     3301 agcattctct cctatttaca cgtatttagg tgcacgcaga tttgtcttcc taccctagag
     3361 gtaggggcac ctcatgtggg attgtcgccc tcccctaacc accttagact cctcaagtgt
     3421 ccgcaagtcg aagatagctt tcctgagaca caatggagtt tgagaatgag caatatgatc
     3481 tccgggatca tactctttag gctacgaggc gagctgatct atctgagtcg cgaggcgcat
     3541 cgcctactgt ccttacattt cccttcgcag ccagaaccac agtcgggagt cctaattcct
     3601 cctcagttgt caagtctgtg tcatgaactg atggttgcac taacgagaag cggcggaggt
     3661 atggactggc aagcggtgcg ggtgcagcaa atggtctctt caccgcaagg tggtgtatta
     3721 ctacgagggt ttggagttcc agatgagctc ccccatgagt gtggtcgctt gcttctgctt
     3781 atcgaaccaa tcattgagca aggagaagca gactcctttt cctcacgaga gcaaatacga
     3841 ttaacgcatc gcgaaggagc tgtagcccgc tacctcgcgg cggggttgac gaacaaggaa
     3901 attgccaatc acctacactt gagtgaatac acggtgaagg accatgtgaa aaggcttatg
     3961 aggaagacac aaaccaccac tcgcacagca accgtaagcc gtctgctcct gcagtatcct
     4021 tccctctcga aagctaaagt cacagacggg atcatgggta cgatcttcgc caaacaagcc
     4081 gtatagcgag tttggccaag agccacgatt cgctcgaccc gaaacagttt gtagcggtgg
     4141 ccctgaaatg acaaatgact ctggcagaaa agccgcacaa gatcagagcg agtgtcactc
     4201 gctcaatcct atgtttgttg gctctctgga atccagagaa aatcggctag gctttcctcc
     4261 ccaatcgccc gatctcaccc ggccttcagt cctctcgact ggcagcaggc caaacagcgt
     4321 cattgaggaa caagctgtgg ctgtttcgcc gtcgactacc aatgagattg gtgtcattac
     4381 agtgtcagcc aaacaacaaa actcacctga cgacagctta ataccactta agaaatttga
     4441 caggaagaaa cgaggagaag ccgcttccag atcggatttg accagatcag ccgcccgatt
     4501 aaggggatga caagaactgc ttgggaagca aggactctac cactgagcta cgcccgcccc
     4561 tgattgaaga gaagtcgaat cctacgctgg caacgacgct aagtcaagga acctgaaagc
     4621 cacttgctca atccttgtac tcggccacgg cgaattttct gcgaccgaca cgcaaacgaa
     4681 gcaactgacc gacgagcaat gggaccgcgc cattcgcatc ggtcatcttc tgctcattaa
     4741 tttccacccc accctgcacg atcaaccgcc gagcttcgct cttgctggga accagtccgg
     4801 tcttcgcgat cagatcgacc agcccgatgg agggtgcgct cgcatctttc acatcggacg
     4861 gcgtgaggac gacatgcgcg tccggctgtt ccggaaactc ccgcgcctga aatttctgct
     4921 gaaacgcggt cctggcttca cggccggcgt cagcgccgtg ataccgggca acgatctgct
     4981 cggccaaccc ctgcttcgca tccataggat gcgccgcctt taccccagcc aaattctcag
     5041 tggtcagtag ctcatagtat cgatgcatga gcgtatcgct gatcgacata agcttgccga
     5101 acatatcagc cggctgatct tccagcgcaa tatagttccc gaagctttta ctcattttgc
     5161 gtacgccgtc agtcccctca agcaacggca tggtgatgac cgcctgcgcc tcctgcccgt
     5221 agtcccgctg gagatcccgg cccatcaaca gattgaattt ttgatccgtt ccgcctaatt
     5281 cgatatctgc tttcagcgcc accgaatcgt agccttggat caagggatac atgaactcat
     5341 ggatgctgat cggcgtgcct tccgtatacc gcttgtgaaa gtcttcccgt tccaacatgc
     5401 gcgcgactgt gtaatgagca ctgagttcga tcaacccgtc ggcggtcatc gtgctcatcc
     5461 aacggctgtt gaactccacc tgcgtctttg cgggatcgag gattttgaag atctgacgct
     5521 catacgtctt ggcattctcc agcacctttt ccttcgacag tgcgacgcgg gtttcggatc
     5581 gcccggtcgg atcaccgatc atgccggtaa aatcgccgat gaggaagatc acctgatgcc
     5641 cgaggtcctg gaaatgcttg agcttatgaa tcaacacggt atggcccagg tgaagatcag
     5701 gtgcagttgg gtcgaacccg gccttgacgc gaaggggacg gttttccttg atcgagcgag
     5761 ccagctttga ttccagctcg ctctgctgaa taacctcaac gatccctcga agtatgagat
     5821 ccaactgctg cgctaacggt gtcacagggt tcctttgtgt cgtgctaacg atttagtgtt
     5881 ggtgacgagg acgtgtggac ggaggcggtc gaacttcttc cggccgatgc ctttcacctg
     5941 gcgcaagtcg tctacagaac ggaaggggcc attctcatcg cgatgctcaa tgactcgttg
     6001 cgctaacttc ggtccgatgc cgggcaacgc ctccaactct gagagggtgg cttggttcag
     6061 gtcgacctgt cgagcaccag cctgtcgagc agaaggcgag tctgttgacg ctttctcgga
     6121 tacacgcgca gatgcagatt cggactcttc aatcggcatc gccacctgtc gacgcgatgg
     6181 cgttcccgcc tgactgacgc ttgccagggc cggcctcgtc gcaggcacct ccaccggtgg
     6241 cggagatgac actgttgtca cggaatgttt cgggagcagt tgaggttgtc ccggcatcac
     6301 cttcgtcacg ggagtgaccg aggcaatcca caccaatgaa cccagcgtca cggcgagcat
     6361 ggcaactttg attagcaatg attgcatcat gtcgttgccg gttttcgcgc gagatggatc
     6421 gcgtgcccat gcacatcttc catggcttcc atgagtccct cggccagggt cgggtgcgca
     6481 tgaatcatct ctgccgcacg cgcgacggtc gcgccaagat gcatcgccag cgcggcttcg
     6541 tgaatcagat cggttgcatg ggcgccgaga atgtggacac cgagaatccg gtcactctcc
     6601 gtatccacca ctacctttac caatccttga atatcccccg tcgcctgggc cttccccaat
     6661 ccgccgtagc ggaaacgtcc tacctttacg gactgttggg gatccttgcc ggcgcccaca
     6721 caacgatcac gggcctgctg ctccgtcaat cccactcgtc cgatttcagg taacgtaaag
     6781 atgcccgtcg ggatgacgtc ataatcgatt gcgcgcgcgt gacccatgaa attctcgacc
     6841 gccaccttgc cttgctgcga ggcgacatgg gctaacatcg ccttacccac aacatcgccg
     6901 atcgcataga ccccggggac attcgtttcc atgcgttcat tgacgacaat ttcaccgcga
     6961 gagccgacct gcactcccgc tttctccagc ccgattccgc gcgaattgaa tcctcgccct
     7021 accgacacca gcacctgctc cacgttcaat gagaggccat cacgaagatg ggccgtcacc
     7081 acgtcggact gccggacgat ctgatcgacg gtaacgccgg tccgaatgtc cactccccgc
     7141 ttcttcaact cccgctccat catctggctg atctcttcat cctccagcgg caggattcgc
     7201 gggaccaatt cgaccagcgt cacctgggct cccagcccgc tatacagaga ggcaaactcg
     7261 cagccctcca cgccaccgcc gacgatgagg aggctggcag ggattcgggt taggtccagg
     7321 gcctgcttgc tggtaatgat ctgagttccg tcgatgggaa agagcggcag gttcggccat
     7381 gaggagccgg tggcgacgat gattccatcg gcggccactt gcaaggtagt cccatccggt
     7441 ttggtcaccc gaatggttcg ggcatccacc aactcacccg tgccctccac atgttcgatg
     7501 ttccaggtct taaacaaagt ggcaatgccc ttgaccagcg tggagacgac tttgttcttc
     7561 ctcgccacca tgatggcagg gtcataggcg accggaccgt tgaggagaag gccgaagtcc
     7621 ttcgcctttt tggccttgtc acccagctcc acgaccgaca acagggcctt gctcggaatg
     7681 cagccccagt tgaggcacac gccgccgagc gcctggtttt cgaccacggt cacacgcgcc
     7741 ccaagctgtg cggcgcgaat cgccgccaca taccctcctg gaccggcgcc aagaattgcg
     7801 agatgtttcg acataagaat gactcagtaa accggtgccg attgaatcac cggctgcaca
     7861 cgatgatgcg tcgccgattc aatggtgcaa ggatgttcgg agttagtgct tctgcttgct
     7921 caaaaactct t
//
LOCUS       DAOQEW010000177         7866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3209_length_7866_cov_7.997824, whole genome shotgun sequence.
ACCESSION   DAOQEW010000177 DAOQEW010000000
VERSION     DAOQEW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3209_length_7866_cov_7.997824"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLS35_15615"
     CDS             <1..213
                     /locus_tag="PLS35_15615"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HNI20151.1"
                     /translation="MAGRRKKLQDYFTDAKLGRSMREHIPLLLSREHIAWIVGQRIDA
                     RFAATASTTRFIVARVTHPVRRKGVL"
     gene            216..767
                     /gene="hpt"
                     /locus_tag="PLS35_15620"
     CDS             216..767
                     /gene="hpt"
                     /locus_tag="PLS35_15620"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNI20152.1"
                     /translation="MERIFGRPIVTQEQMRTRIRELGRQIASDYAGKELVLVGVLKGA
                     YAFYADLARAIRIPMRVEFIVVTSYGAGKRSSGKVKLISDLTEPIVGKDVLLVEDIVD
                     SGLTVQYLMKTLSRRKPRSLKVCTLLSKPERRQVEVDLEYVGFKIPNKFVVGYGLDYR
                     QEYRNLPYLAALDQGEEGEQESA"
     gene            855..2669
                     /gene="ftsH"
                     /locus_tag="PLS35_15625"
     CDS             855..2669
                     /gene="ftsH"
                     /locus_tag="PLS35_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNI20153.1"
                     /translation="MNSRVKNLLFWVVVGLFMILLFNLFSVPTHAPEDEVIFSDFMAK
                     LDKGEVMKVTIKANHISAILKDQSRIRTYTAEYPELVKHLREKDVQIEARPPDESPWY
                     ITFLVTWGPFILFLGLWFFLMRQMQIGGNKALSFGKSRARMLTEERKKVTFSDVAGIE
                     EAKEEVLEIIEFLKDPRKFQKLGGRIPKGVLIVGPPGTGKTLLAKAIAGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRLRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTTEGVILIAATNRPDVLDPALLRPGRFDRQVVVNRPDLRGRSEILK
                     VHTKKVPLSTDVELEKIARGTPGFSGADLENLVNEAALWAARLNKKEVELIDFEMAKD
                     KVLMGAERKSMVLTDEEKRTTAYHEAGHALMAKLLPGTDPVHKVTIIPRGRALGVTMQ
                     LPTDDRHNYSKDFLYNNLAILMGGRVAEELVLHDVTTGAGNDLERATDLARKMVCEWG
                     MSEKLGPLTFGRKEEEIFLGRELGSKRDFSEQVALEIDHEVRRLVTENYERAKRILTD
                     NMTSLKALAEALLEKEVLDALEIDQILIQGSSSQTVPA"
     gene            2857..3756
                     /gene="folP"
                     /locus_tag="PLS35_15630"
     CDS             2857..3756
                     /gene="folP"
                     /locus_tag="PLS35_15630"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNI20154.1"
                     /translation="MTLDTPSPFRPVVITAGSHNITIQSNPLVMGVLNVTPDSFSDGG
                     AYSTVERAVDHARQMQADGADIIDIGAESSRPGADPVEEGEELRRLIPVVEAVREAVS
                     LPISVDTTKAAVARLAIQAGALIVNDISALQGDPLMAGLVAETGVAVVLMHMQGTPRT
                     MQRSPRYGNVVEEVTVFLRQRAQEAIARGIRPTQIILDPGFGFGKLQEHNLHMLAEFD
                     AFTKLGYPVLAGVSRKQFIGNLIRQPVHEREFGNAGAVAVAVLKGAHIIRVHDVRAMK
                     ETATVVSAISRHARSSSVEVSNA"
     gene            3749..5098
                     /gene="glmM"
                     /locus_tag="PLS35_15635"
     CDS             3749..5098
                     /gene="glmM"
                     /locus_tag="PLS35_15635"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNI20155.1"
                     /translation="MRKLFGTDGVRGVANLEPMTSEIAMQLGRAAAHIFMRRAGRHQV
                     VIGKDTRLSGYMLESALTSGICSMGVDVLLVGPLPTPAIAFLTRSLRADAGVVISASH
                     NPYQDNGIKFFSNEGFKLPDEVEARIEQLIISDEIKHLRPTADAIGKAYRIGDAEGRY
                     IEFVKRSLPRDLDFQGIKLVVDCANGAAYKVAPAVLRELGADIEVIANTPDGMNINDH
                     CGAVHPERLQEAVRRNGAHIGIALDGDADRAIFVCEQGHIVDGDHVMAALGLDLHGQG
                     RLASQTVVGTVMSNFGLELAMKKAGIQLMRTPVGDRYLMERMLADGYNFGGEQSGHFI
                     FLDHNTTGDGLISALQILSLMKRTGKPLSELAKAMTAVPQILLNVKVKHKPDLNQIPD
                     IQQAIKTAEATLNGSGRVLVRYSGTEALLRIMVEGERDATIREVADYLADIVRARIG"
     assembly_gap    5165..5264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5265..7265
                     /locus_tag="PLS35_15640"
     CDS             <5265..7265
                     /locus_tag="PLS35_15640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNI20156.1"
                     /translation="AGAIEVLGADEGLLAYPISSRIDAWRSQVRNAADALPQPARGLF
                     LSLTIGEQGFLAPEVREWFMTTGTVHILSISGSHLGLIALLSFALVRKACLLLPSMLL
                     LGLSRWLIPSRLAALLTLVPVLGYTLLAGAETATIRSAIMIIVGLWTIWLGTPQYLLH
                     ALAAAAGLTLLVHPAALYDISFQLSYVSVWVLALALQRQQPTNEFPLPQKHLAGRTLY
                     WLQESVRLTAWVTLATLPLVACYFNQVSWMGLFANLLMVPFVGFILLPISLLSAVWVI
                     VSQSSTLPGAGLIEFLAQAVTVPTQWLAGLPGAEWFVAAPTVPLMALFYLLGWAMLTD
                     RPAHAPLLVKRAMAFGMACLILWWLWSPRPFTREGQVRITFLDVGQGDSAVIELPKGE
                     VVLIDGGATYERFDMGRSVVAPFLWNRGIRTIDHVIATHPQLDHAGGLAWILAHVQVE
                     NFWTNGVTRSEEFWRKIERTLARRHLQAQVAEQGRRLVDDAECDIAVLNPPPGRNASS
                     AGKQQSLNNLSVVTELTCGGRRMLFTGDIEREGLARMVQSGHLRHIDLLKVPHHGALS
                     SLDREWLSSVQPDIAVVSAGRRNPYGHPAGEVIAAYESLETQLWRTDQDGAVWADLDL
                     NKQSLTMHSTREWQLQPALIPIPQWAIEQDNFHRLWRRWNWN"
     gene            7577..>7866
                     /locus_tag="PLS35_15645"
     CDS             7577..>7866
                     /locus_tag="PLS35_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20157.1"
                     /translation="MMANRLLFLAPVPPSDHQGGGPLRMLHLLRFLGSRFHVDLVAPA
                     VDGWENAQNSLQGVCAETTFVPPQGPGLLDRLGRVSPYVKDRALASVVRERLA"
BASE COUNT         1575 a         2043 c         2395 g         1753 t
ORIGIN      
        1 atggccggtc gacgaaagaa attgcaagat tacttcacag atgcaaaact gggtcggtct
       61 atgcgagaac acatcccact gctgttgtct cgagaacaca tcgcctggat cgtggggcaa
      121 cggatcgatg cccgattcgc ggccaccgct tcgacgaccc gatttatcgt cgctcgtgtg
      181 acgcatcccg tgcgccggaa aggagtcttg taggaatgga acgcattttt ggtcgcccca
      241 ttgtgacgca ggagcagatg cgaactcgaa tccgtgagct cggccgacag atcgcgtctg
      301 actatgccgg caaagagttg gtgctggtag gggtgctcaa aggagcgtat gccttttatg
      361 ccgacctggc ccgagccatc cgtattccga tgcgggtaga atttatcgtg gtgacgagtt
      421 atggcgccgg caagcgaagc tccggtaagg tcaagttgat ctccgacctg acggaaccga
      481 tcgtgggcaa ggatgtcctc ttggtcgagg acattgtcga ttccggtctc acggtgcagt
      541 acctaatgaa gactctgtct cgacggaaac ctcggtcgtt gaaagtctgc acgctgctca
      601 gcaagccgga gcggcggcaa gtcgaggtgg atctggaata tgtcgggttt aaaattccca
      661 ataagtttgt ggtggggtat ggtctcgatt accggcagga gtaccgcaat cttccgtacc
      721 tggcggcgct tgatcaaggc gaggaagggg agcaggagtc cgcatgaaag gttgttcccc
      781 ctgcggaacc tatggtaaca ttctgcaggc ttagctaatt ttcggtctgc ctaatatcct
      841 caaaggagag gtggatgaat tcacgcgtaa agaatttatt gttctgggtc gtggtcggcc
      901 tgttcatgat tttgttgttc aatttgttta gcgtgccgac ccatgcgccg gaagatgaag
      961 tcatattcag cgatttcatg gccaagctcg ataagggcga agtcatgaag gtcacgatca
     1021 aagccaacca catcagcgcg attcttaaag accaaagccg gattcggacc tataccgctg
     1081 aatacccgga attggtgaag catttgcgcg aaaaagatgt gcagattgaa gcgcggccgc
     1141 ctgatgaaag cccgtggtac atcaccttcc tggtgacgtg gggccccttc attctgttcc
     1201 tcggactgtg gttcttcctc atgcgccaga tgcagatcgg tggcaacaag gccttgtcgt
     1261 tcgggaaaag ccgcgctcgc atgttgaccg aagagcgtaa gaaggtgacg ttctcagatg
     1321 tggccggcat cgaggaagcc aaggaagaag tactggagat tattgaattc ctgaaagatc
     1381 cgcgaaagtt ccagaagctc ggcgggcgca tcccgaaagg cgtcctgatc gttggccctc
     1441 cgggaaccgg aaagaccctc ctggccaagg ccattgccgg cgaagcgggg gtgcccttct
     1501 tcagcatcag tggttcagac ttcgttgaaa tgttcgtcgg ggtcggagct tcccgcgtac
     1561 gggacctgtt cgaacagggc aagaagcacg cgccttgtat catcttcatc gacgaaatcg
     1621 atgcggtcgg tcgtctgcgg ggcgcgggcc ttggcggagg acacgacgag cgtgagcaaa
     1681 ccctgaacca attgctggtc gagatggacg ggtttgacac gacggaaggc gtcattctca
     1741 tcgctgccac gaaccgtcct gacgtgctgg atcccgcctt gttacgtccc ggtcgtttcg
     1801 accgtcaggt ggtggtgaac agaccggacc tgcgtggccg atcggaaatt ctgaaggttc
     1861 ataccaagaa agttcctttg tcgacggatg ttgagctgga aaaaattgct cggggtacgc
     1921 cagggttctc cggggccgac ctcgaaaatc tagtcaacga agcggcgctg tgggcggctc
     1981 gtctgaataa gaaggaagtc gagctgatcg acttcgaaat ggcgaaggat aaggtcctga
     2041 tgggtgcgga acggaagagc atggtgctca ccgatgaaga aaagcgcaca acagcctatc
     2101 atgaagcagg ccatgcgctc atggcgaagc tcttgccggg aacggatccg gtgcataagg
     2161 tcacgattat tcctcgcggc cgtgcactcg gcgtgaccat gcagcttcca actgatgatc
     2221 gtcacaatta ttccaaggat ttcctctaca acaatctcgc cattctcatg ggcgggcggg
     2281 tggcggagga gttagtgttg catgatgtga cgaccggagc gggcaacgat ttggagcgcg
     2341 cgacggatct cgccagaaaa atggtctgcg aatggggcat gagtgaaaag ctggggccgc
     2401 tgacctttgg acggaaggaa gaggaaattt tcctcggtcg agagcttggg tccaaacggg
     2461 atttcagcga gcaggtcgcc ctggagatcg accatgaagt caggcgcctc gtgacggaaa
     2521 actatgaacg tgcgaagcga atcctgaccg acaatatgac cagtctgaag gcactggccg
     2581 aggcgttgct ggaaaaagaa gtgctggacg cattggagat cgatcagatc ctcattcagg
     2641 gatcctcctc acagacggtt cctgcctagt ttgtaaactg ccggcttcac aggggcaccc
     2701 atgggtgttc ctgtgaagca tgttcctcat atgagttcca ggccaagggt tgggacatcc
     2761 tgctgtcacc tcatcggtgc atgatctccc atcccttatc tcgtcagctt acatcattcc
     2821 atcgtgtgcc gttctgagag cgccttagga cttctcttga ccctcgacac tccatctcca
     2881 ttccgtcctg tggttattac ggctgggtcc cataacatca ccattcaatc gaatccactc
     2941 gtcatgggtg tgctcaatgt gacgcctgac tcattttccg atgggggggc ctactccaca
     3001 gttgaacggg cggtcgatca tgcgaggcag atgcaagctg acggggccga tatcatcgac
     3061 atcggcgccg aatcctcccg gccgggagcg gacccagtcg aggaaggtga ggagttgcgc
     3121 cgcttgatcc ccgtcgtgga agccgtgcgg gaggctgtat cccttcccat ttcagtcgat
     3181 acgacgaagg cggctgtggc tcgtttggct attcaggcag gggcccttat cgtaaatgat
     3241 atcagcgcct tgcagggaga tcccttgatg gctggcctgg tggctgagac cggggtcgct
     3301 gtggtcttga tgcatatgca gggcacgccg cggaccatgc aacggtcgcc acggtatggg
     3361 aacgtggtgg aagaggtcac cgtatttttg cggcagcggg ctcaggaggc gatcgcccgc
     3421 ggtatccgtc caactcaaat catccttgac ccgggcttcg gttttggtaa gctccaagag
     3481 cataatcttc acatgctcgc agagttcgat gcgttcacga agttggggta cccggtgctg
     3541 gccggggtat ctcgtaaaca gtttatcggg aatctgattc ggcaaccggt tcacgaacgg
     3601 gaatttggta acgccggggc tgttgcggta gccgttctga aaggcgctca cattattcgt
     3661 gtgcatgacg ttcgagcgat gaaagagacg gccacggtcg tgtcagccat ttcccgtcac
     3721 gcgcgttctt cttctgtaga ggtatccaat gcgtaaattg ttcggcacag acggtgttcg
     3781 cggggtcgcg aacctggagc ccatgaccag cgaaatcgcc atgcaactcg gccgtgccgc
     3841 ggcccatatt tttatgcgcc gggcgggcag gcatcaggtc gtgatcggaa aagacacgcg
     3901 cttgtccggg tacatgctgg aatcggcctt gacctcgggc atctgctcga tgggcgtcga
     3961 cgtattactc gtgggacctc tgccgacccc cgccattgcg tttcttactc gaagtttacg
     4021 cgcggatgcg ggcgtggtga tttctgcctc gcataatccg tatcaggata acggaatcaa
     4081 gtttttctcc aacgaaggtt tcaaactgcc tgacgaggtg gaagcgcgga ttgaacaatt
     4141 gattatttcc gatgagatca agcatctgcg gccgactgcc gatgcgatcg gaaaggcgta
     4201 ccggatcggt gatgccgagg gacgatatat cgaattcgtg aaacggtcac ttccccgcga
     4261 cctcgatttt caaggaatta agttagtggt ggattgcgcc aacggcgcgg cctacaaagt
     4321 ggcccccgcg gtattgcggg agttgggtgc cgacattgaa gtcatcgcca atacgcctga
     4381 cgggatgaac atcaacgacc attgcggggc ggtgcatccc gagcgactgc aggaggccgt
     4441 gcgccggaac ggtgcgcaca tcggtatagc gttggacggc gacgcggatc gagccatctt
     4501 cgtctgcgag cagggccaca ttgtggacgg cgatcatgtc atggccgcat tagggctcga
     4561 tttgcatggt caaggccggc tcgcttctca gacggtcgtg ggaaccgtga tgagcaattt
     4621 tggattggaa ctggccatga agaaggcggg cattcagttg atgcgaacgc ccgtgggtga
     4681 ccggtatctc atggagcgga tgttggccga tggctataat ttcggcggcg agcaatccgg
     4741 ccactttatt ttcttggatc ataacaccac aggcgacggc ttgatttcgg ccttgcagat
     4801 cctctccttg atgaaacgca cggggaaacc gctgtctgaa ctcgcgaagg ccatgactgc
     4861 ggttccgcag attttgctga acgtcaaagt gaaacacaag ccggacttaa accagattcc
     4921 ggatattcag caggcgatta agacggcgga agccacgttg aacgggagtg gccgtgtgtt
     4981 agtgcggtat tccggcaccg aagcgctgtt gcggatcatg gtcgaagggg aacgggatgc
     5041 gaccattcgt gaggtcgccg attaccttgc ggacattgtt cgcgctcgta tcgggtagta
     5101 ccggctcctt gcccgaggcg tcggatgctg ccgttcctga cgatcatctt catcctcgga
     5161 atcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccggt gccattgaag
     5281 tgctaggtgc agatgaggga ttgctggcat atcccatttc gtcgcgcatc gatgcatggc
     5341 gcagtcaggt acggaatgcg gcagacgcgc tgccacagcc ggctcgaggt ctgttcctca
     5401 gcttgaccat cggggagcag ggcttcctcg cgccggaagt acgcgaatgg ttcatgacga
     5461 ccgggacggt gcacatcctt tccatctccg gatcgcatct tggactgatc gcactgctgt
     5521 cttttgcgtt ggtgaggaaa gcctgcctcc tgcttccttc catgctgctc ttgggacttt
     5581 cacgatggtt gattcccagt aggctggcag cattgctcac tctggtgccg gtcctgggct
     5641 ataccctgtt ggcgggtgcg gagacggcca cgattcgttc ggccatcatg atcatagtgg
     5701 ggctgtggac gatatggttg ggcacgccgc agtatcttct tcacgccttg gcggcggccg
     5761 ccggcttgac gctgctggtg caccctgccg cgctttatga tatttcgttt caactatcct
     5821 atgtgtccgt gtgggtactg gcgctggcac tgcagcgaca gcaacccacc aacgaatttc
     5881 ccctgccaca gaaacacctc gctggccgca ccctctactg gttacaggaa tccgtccggc
     5941 tgacggcctg ggtgacattg gcaaccttgc cattggtggc ctgctacttc aatcaagtgt
     6001 cctggatggg gcttttcgca aatctgctca tggtgccttt cgtgggattc attctgttgc
     6061 cgatcagtct gctgtccgcc gtgtgggtca ttgtttcgca gagcagtacg ttgcccggtg
     6121 ccgggctcat agagttcctc gctcaggctg tgacggttcc gacgcagtgg ctggctggtc
     6181 tgccgggagc ggaatggttc gtcgccgcac cgacggttcc cttgatggcg ctgttctacc
     6241 tgctaggctg ggccatgctg actgacaggc cggctcatgc gcccctgctg gtgaagcgcg
     6301 ccatggcgtt cggcatggcc tgtctcatcc tctggtggct gtggtctccg cgtccgttca
     6361 cgcgggaggg gcaggtgcgg atcacgtttc tcgacgtagg gcagggagat agtgccgtga
     6421 tcgaattgcc caagggggag gtcgtgctca tcgatggtgg tgccacgtat gagcgctttg
     6481 acatgggacg cagcgtcgtc gcgcccttcc tctggaatcg cggtattcgc accattgacc
     6541 atgtgatcgc gacgcatcct cagttggatc atgccggcgg actagcctgg atcttggcac
     6601 atgttcaggt agagaacttc tggaccaatg gtgtcacgcg aagcgaggaa ttctggcgca
     6661 agatcgagcg gacgttggcc cgacggcatc tccaggcgca ggtcgctgag caggggagac
     6721 gactggtgga cgacgcagaa tgtgatattg ccgtgttgaa tccaccgccg ggccggaacg
     6781 catcctctgc gggaaaacag caatcgttga ataatctgtc ggtcgtgact gagctcacct
     6841 gtggcggtcg acgaatgttg ttcacagggg acatcgagcg ggaagggttg gcccgtatgg
     6901 tgcaatccgg gcatcttcgt catatcgatt tgctcaaagt tccacaccac ggagcgctga
     6961 gctcattgga tcgtgaatgg ttgagcagtg ttcagccaga tatcgccgtg gtatctgcgg
     7021 ggcgccgcaa cccatatggg catccagcgg gtgaggtcat cgcagcctac gagtcccttg
     7081 agacgcagct gtggcgaacg gatcaggacg gcgccgtgtg ggctgatctt gacctgaaca
     7141 agcagtctct gaccatgcac agcaccagag aatggcagct tcaaccagcg ttaatcccga
     7201 tccctcagtg ggcgattgag caggataatt ttcatcgcct gtggcgccgg tggaactgga
     7261 actaggcacg gtcccggtct gagcatcgtg attcttatcg ctcccgccgg gcagaaatcc
     7321 ccagttgcat ggctcccgaa tgtgccgggt gccccgtttt tcaccaatgc ggctgtcaat
     7381 tttgccggtg tggggaaaac atgtcggcat aagttggcaa tattgcgaat ggaaccggga
     7441 gtgtcatcaa ggcatagcac atgcagagat gttcaacgcc tggtcacggc agatcttccg
     7501 ctgacggtgg ggcatagctg accccctgcg gacgataccg cagcccccag gataaagagc
     7561 gagagaaggt taggcgatga tggccaatcg actgttgttt ctagccccgg ttccaccctc
     7621 cgatcaccag ggcggtggtc ctttgcggat gttgcacctg ctacgttttc tggggagccg
     7681 ctttcacgtg gacctggtcg ctccggcggt tgatggctgg gagaatgcac agaactcatt
     7741 gcagggcgtg tgcgcggaga cgacgtttgt tccgcctcaa ggtcccggat tactggatcg
     7801 gctcggacgg gtcagcccct acgtgaaaga tcgcgccttg gcttctgtcg tgcgcgagcg
     7861 gttggc
//
LOCUS       DAOQEW010000178         7828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3240_length_7828_cov_8.222951, whole genome shotgun sequence.
ACCESSION   DAOQEW010000178 DAOQEW010000000
VERSION     DAOQEW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_7828_cov_8.222951"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..781)
                     /locus_tag="PLS35_15650"
     CDS             complement(422..781)
                     /locus_tag="PLS35_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20158.1"
                     /translation="MNSFNDFLLRMGGMHDSIVERLMWSPDEKRIELQFDDLYRNFNG
                     LPEYPGRQPGVIVLHGVSNLSLTLDSDGPLRVFEFLPEEGESDVVVLTFSPTGKLRVQ
                     YRSVDHPACLINAVGRS"
     gene            complement(1242..1817)
                     /locus_tag="PLS35_15655"
     CDS             complement(1242..1817)
                     /locus_tag="PLS35_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20159.1"
                     /translation="MLSKSASLIFRLLFVVAALGAQCSFANDACSSFSAADITTYLPD
                     GEGYWMRGRNGPNGSAISVCTERGKGVPVAFISEDSAQEFADLHILSIQSFYGLLIVT
                     QESGAAYRIRFFAYDIKANQVREMASDGSKFLPSLGYLGSELQPVVGVIHSALDSRVS
                     SQPLPKEEVLYFLDKSKMNISSKRKAWSAKR"
     gene            complement(1793..3175)
                     /locus_tag="PLS35_15660"
     CDS             complement(1793..3175)
                     /locus_tag="PLS35_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNI20160.1"
                     /translation="MTGLSTLRACLLGTSCLLAASAAHADTYNYDELGNVRQIQNDKG
                     VRTYTFDEVNRLKTESGLAGVRTHTYDPNSNRLNDGAPSPTTASFAPASDRIATLNGV
                     TVTLDAAGNLLGDGTYTYAWNDAGQLLSVSKAGQLVAKYYYDYKGRRTRKATTAIAPQ
                     GAQVFVYHYDASDHLIGETLGDATPLRTYVWLNDQPNAVIEHPALSTPQRTLYLQVDH
                     LGTPRLAKDSTGKIQWRWDSDGYGSTAADEDPDGDGKKTTINLRFAGQYYDVESGLHY
                     NWNRYYSPRLGRYISSDPIGVEGGGNTYGYVGGNPLSYFDVLGLSPMSPTEIAQIIKD
                     NNHSPLSNEMVMCLMWQESGFDPDVENDGARGLMQIRKNQALAQVNKKSGPFAWQDLF
                     DPSTNTEVGTLYLNWVSQRPGDLDRNIQRRYGTGDDYPVAKMRQCEKCLQETCSKDGD
                     VKKCLAKVHR"
     gene            complement(3190..3543)
                     /locus_tag="PLS35_15665"
     CDS             complement(3190..3543)
                     /locus_tag="PLS35_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20161.1"
                     /translation="MFRSILRLALLCGTLSSPVAQSAALSCTGTLTDVANSAAGELRI
                     KPSWRGDWITLCSTAVVLNGVQTEACKRWQAQALAAQLTAANTWVQYDNPVAPSCATM
                     GTLGNADRPGYLVTY"
     gene            complement(3556..3906)
                     /locus_tag="PLS35_15670"
     CDS             complement(3556..3906)
                     /locus_tag="PLS35_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20162.1"
                     /translation="MNRRALILTLMMTLPALPAAAGTLACNGTIAQVMTTASGDLRVV
                     PSWRNDWVTLCNVVSPWKGVPVDACKRWHAQALAAQIAQTNTQTAYASTAATACAQMA
                     VASAADAPDTFSNN"
     gene            complement(3925..7512)
                     /locus_tag="PLS35_15675"
     CDS             complement(3925..7512)
                     /locus_tag="PLS35_15675"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HNI20163.1"
                     /translation="MFIKFEENRKLESIDLCKFFFFRSAFAAYVVCASSYVFAQVAYM
                     EPTVPIVTTPWKDGWITAGGPQEICQQIFIEAAGNPPERPITMVLSSLRIIPEPNHNY
                     SVSDFRLGEVLPYDTPNFSAWYCSASYDHYDYGGLHHHNGDLGNSGEFLYPVCPNHPY
                     PGTYPLGEPNPPKYCTGPTTPVRPPVCNEIGYPVEPASGVKVFRELDFRHSSNPHLYL
                     ERTYRSFFRIQPAGMVSWITNWHRRLFFQEIEKDVSVVSVDESGRSRYFYTSQLVPEH
                     GLLDRLTKTSSGYQLFRADNTLEQYTSDGYLAGVYHHDGAYEIIRYVGGSAVGSWNVA
                     SITDHNGAAIKFNYDAVDRVSSVTTSDQRVINYLYFNYNGYPLSVVGWQDGKSRSYTY
                     SVESPSSWMNILSVVDEDGALFSRVEYDRNNRVVYSGRGGDQSSYTFKYSEDKWFPTS
                     TVVTDPSGVQRTYSSVLDKSQIFPTGVSKPGGAGCNASSSNIDYDTFGNAISRTDFNG
                     MKSCHGYDASRNLETSRIEGFASSDACPADVLSSAPSSTQRKTTTQWHPFWRLETRQA
                     EPKRVTTWVYNGQPDPTNGNALASCAPATAKLPDGQVIAVLCKRIEQATTDATGAQGF
                     GASADGPARIWAYTYNAFGQVLTEDGPRTDLADVTKYVYYTDTTANWTQGDLRSITNA
                     LGHVWTFTQYDKAGRLLAATDPNGTALTYTYTPRGWLASRSVAGQTSTFAYTGWGGLA
                     LVTLPDGTSTSYTWDAAHRLTSVSDSAGNSTRFVLDPSGNITSSTTTDAAGNVARQTR
                     TEFDALGRPWKHYDAAGHAFEVWHDAMDRVAKTIDPKGRTTTFARDTLGRLQDITDAQ
                     TTPGHTGFAFDARDQMTRQTAPNGAVTQLSVDGLGNTLQEVSANRGTLRFTYDAAGNL
                     LTRTDARGVTANYTYDALNRIRKRITKNSAGVVTETLDYIWDGASGCTYGRGHLCQIT
                     DGGGVTNFAYDARGNRISERRSEAGRSYTTQYTLNGADRIVALVTPTGSLLDQARDAG
                     GRVAALTATAGGSSIPVASAVRYDGAGNVVSLSQGTSLSQLMQRNADGQLEANGLGIP
                     VTGLGLSLGKRFIRPGDGLDFTLTLTPAQASGTVFLCQLDCQGSNQLAVIPVTGGTTT
                     GTLTGLTKGVHHLSAVLVPNPPYAASLSESRRVLVGVPPTLFFDDLFLQ"
BASE COUNT         1661 a         2219 c         2215 g         1733 t
ORIGIN      
        1 gccggccgcc gcgcgtggat ggaagacaag ggggattcgg tggaggcgga tgtgtaggta
       61 cgtgcagcac ttccatgtcc tgctggctga tgcagccggt ttcgatgcca ccgatgacgg
      121 cagccaggta gctggcgcgg gtttcttcgt agtactcgtt catcttgctc atgtcgcatc
      181 ctttgcattt cggttgaaac gcgcacggac acccgtccgc acgcggcaaa cggcacgcgc
      241 aaggccggca ggcgctatcg acttaggaag ggaattagag cgatacccgc acggcgctta
      301 cgcgtgcgac ggtatctgag cgactgctga gacgaacaac acgccagggc ggcgcgtcgt
      361 gtcgaagcgg gggaacggtc tgttcatagg ctagatcacc aggatgagca ggggcggttt
      421 gtcaagagcg gcccactgca ttaatcaggc atgctggatg gtcaacggac ctatattgga
      481 cgcggagctt tcccgttggc gaaaaagtca aaactacaac atctgactcg ccttcttcgg
      541 gcaaaaactc gaatacccgc agaggtccgt cagaatcgag tgtaagactc agattgctaa
      601 caccatgtaa aactataact ccgggctgcc tgccagggta ctctggcaat ccattgaagt
      661 tacgatacaa atcatcaaat tgaagttcaa ttcgtttttc gtctggcgac cacataaggc
      721 gctcaacgat agagtcgtgc atacccccca ttcgcaaaag aaaatcatta aagctattca
      781 taattacctc atggcgcagc agcattaccc gggatggtcc accaaagcgg aggaacaagt
      841 gatggaatca tacgtacgca acgatagatg atgtaagcgc cagtcgctga cgtgacaacc
      901 gttgcaacct tctgtttgca atcatccccg catgtttttc cgtcgctcgg tgaatccggc
      961 acttcagtcc caggaggcaa atgctcatct cctccattgt ggtgccaatg gtctttacct
     1021 ctccagccag gcataccagg ccgaccttgg tgccaatcaa gaacatcccc attttggttt
     1081 gtccatctct cgccgttagg gtcgcggtgt ctagtatcac gttgccaatc gctaggtagg
     1141 ccactcgggt catttggacg tttgatttcc cccgttgggt ccgtatagct cagcggattc
     1201 ccgccgacat agccatagat gttttgcctg ttttttccgg cttagcgctt cgcactccat
     1261 gccttgcgtt tagacgagat attcatcttg ctcttgtcga gaaaatatag gacttcttcc
     1321 ttagggagcg gctgagaaga aactctcgaa tctagcgcag aatggatgac cccaaccacc
     1381 ggttgaagtt ctgacccaag gtagcccagt gaaggcaaaa actttgaacc atcacttgcc
     1441 atctctctta cttggttcgc cttaatgtca tacgcaaaaa aacgaatccg ataagcggcg
     1501 ccagattctt gtgtaacgat aagcagccca taaaacgatt gaatgctgag aatgtgcaag
     1561 tcagcaaact cttgtgcgct gtcttcactt ataaaggcga caggcacccc ttttcctctt
     1621 tcggtacaga cactaattgc agaaccattt gggccattgc gtccgcgcat ccaatatccc
     1681 tcgccgtccg gaaggtaggt ggttatatcc gctgcagaaa aactgctgca agcatcgttt
     1741 gcaaaagagc attgagcccc caaggctgca acaacaaata gcagtctaaa aatcaacgat
     1801 gcacttttgc taagcatttt tttacatccc catcttttga gcacgtttcc tgtagacact
     1861 tctcgcattg ccgcatcttt gcgacaggat aatcatctcc agtaccgtat cttctctgga
     1921 tatttctatc taagtccccc ggtcgttgag agacccaatt gagatagagc gttccgacct
     1981 cagtgtttgt tgaagggtca aacaagtctt gccaagcgaa aggtccactc ttcttgttta
     2041 cttgcgccag cgcctgattc ttccgaattt gcattaagcc acgtgcacca tcattttcaa
     2101 catccgggtc aaaccctgac tcttgccaca tcaggcacat aaccatctca ttgctcagcg
     2161 gcgagtgatt gttgtcctta atgatttggg caatctccgt aggcgacatc ggcgacaatc
     2221 cgagcacgtc gaaataagag agaggattcc cgccgacata cccataggta ttgccccctc
     2281 cctccacccc tatcggatca ctggaaatgt accgcccgag cctcggacta tagtacctgt
     2341 tccagttgta gtgcagcccc gactccacat cgtagtactg ccccgcaaac cgcaggttga
     2401 tcgtcgtctt ctttccgtcc ccgtccggat cctcgtcggc cgcggtgctt ccgtagccgt
     2461 cgctgtccca gcgccattgg atcttgcccg tgctgtcctt ggccaggcgg ggcgtgccca
     2521 ggtgatccac ctgaaggtac agcgtgcgct gcggggtgct cagcgccgga tgttcgatca
     2581 ctgcgttggg ttgatcgttc agccaaacgt aagtgcgcaa cggcgtcgca tcgcccaagg
     2641 tctcgccgat caggtggtcg gaggcgtcgt agtgatacac gaacacctgg gcgccttgcg
     2701 gggcgatggc ggtggtggcc ttgcgcgtgc ggcgtccctt gtaatcgtag tagtacttgg
     2761 cgaccagttg cccggccttg ctcaccgaga gcagttgtcc cgcgtcgttc caggcgtagg
     2821 tgtaggtgcc gtcgcccagc agattgcctg cggcgtcgag agtaacggtc acaccgttga
     2881 gggttgcgat ccggtccgat gcgggggcga agctggccgt ggtcgggctt ggggcgccat
     2941 cgttcaggcg gttgctgttg ggatcgtagg tatgggtgcg caccccggcc aatccgctct
     3001 cggtcttgag acgattcacc tcgtcaaagg tgtaggtccg gacgcctttg tcgttctgga
     3061 tctgtcgaac gtttcccaac tcgtcatagt tgtaggtgtc tgcgtgagcg gcgctcgccg
     3121 ccaataggca gctcgtgccc agcaggcatg cgcgcaacgt ggaaagacca gtcatctcga
     3181 ggcgcctcgt cagtacgtca ccagatagcc gggacggtct gcgttgccga gtgtccccat
     3241 cgttgcgcaa ctcggcgcga ccgggttgtc gtattgaacc caggtgttgg cagccgtgag
     3301 ttgcgcggca agggcttggg cctgccagcg cttgcacgct tcggtctgga ccccgttcaa
     3361 tacgaccgca gtactgcaca gggtgatcca gtccccccgc caggagggtt tgatccgcag
     3421 ttcgcctgca gccgagttcg cgacatcggt cagggtgcct gtacaggaca gtgcagcaga
     3481 ctgggcgacg gggctggaga gggtgccgca cagcaatgca agtctcagaa tggagcggaa
     3541 catgtctgcc gggcctcagt tgttgctgaa ggtatcgggg gcgtcggccg ctgaggcgac
     3601 cgccatctga gcgcacgccg tggctgccgt gctggcatag gccgtctggg tgttagtctg
     3661 ggcgatctgg gcagccaggg cttgggcatg ccagcgtttg caggcgtcca ccgggacgcc
     3721 tttccagggg cttacgacgt tgcacagggt gacccagtca ttgcgccagc tgggcacgac
     3781 gcgcagatcg ccgcttgcgg tcgtcatcac ttgcgcaatc gtgccgttgc aggccagcgt
     3841 ccccgctgcg gcaggcaggg cgggtaatgt catcatcagg gtcaggatca gggcgcgacg
     3901 gttcatgaat gcggtcctcg ggcgttactg caggaagaga tcgtcgaaga agagggtcgg
     3961 cggtacgccg acgagtacgc ggcgcgactc ggacaggctg gcagcgtaag gagggttggg
     4021 cacgagtacg gcgctcaggt ggtgtacgcc tttggtcagg ccggtgagcg ttcccgtcgt
     4081 tgttcccccc gttacgggaa tgaccgccag ttggttgctg ccctggcaat cgagttggca
     4141 caagaacacg gtccccgagg cctgggcagg cgtcagggtc agggtgaagt caagcccatc
     4201 ccccggacgg atgaagcgtt tgcccaggct cagccccagg ccggtgaccg ggatgcccag
     4261 cccgttcgcc tccagttgtc cgtcggcgtt gcgctgcatg agttgggaca gactggtgcc
     4321 ctggctcaga ctcacgacgt ttccggcccc gtcgtagcgg actgcggatg ccacgggaat
     4381 cgatgaacca ccggcggtgg ccgtcagcgc ggcaacacgc ccgccggcgt cccgcgcctg
     4441 atcgagcagg gatccggtcg gcgtcaccag ggcaacgatg cggtctgcgc cattgagtgt
     4501 gtattgagtc gtgtagcttc taccggcttc gctgcggcgc tcgctgatcc gattgccccg
     4561 cgcatcgtag gcaaagttcg tcaccccgcc gccatcggtg atctggcaaa ggtgaccgcg
     4621 tccgtaggta cagccggatg ccccatccca gatgtagtcg agcgtttcgg tcacaacacc
     4681 tgcgctgttc ttggtgatgc gcttgcggat gcggttgagc gcatcgtagg tgtagttcgc
     4741 agtgacgccc cgcgcatcgg tacgtgtcag caggttgccc gccgcgtcgt aggtgaaacg
     4801 cagggtgccg cgattggcac tgacctcctg gagggtgttt ccgaggccat cgacgctcag
     4861 ttgggtcacg gcgccgttgg gggcggtttg ccgggtcatt tggtcccgcg cgtcaaaggc
     4921 gaagccggtg tgaccgggcg tggtctgcgc atcggtgatg tcctgaagcc gtcccagcgt
     4981 gtcgcgtgca aaggtcgtgg tccgcccttt gggatcaatg gtcttggcaa cccgatccat
     5041 cgcgtcgtgc cagacctcga aggcatggcc ggctgcgtca tagtgcttcc aggggcgccc
     5101 gagcgcatcg aactccgtcc gtgtctggcg agcgacattg cccgcagcgt ccgtggtggt
     5161 cgaagaagtg atgttgccgg acgggtcgag cacgaagcgc gtgctgttgc ctgcgctgtc
     5221 ggaaacactg gtcagccggt gagcggcatc ccaggtgtag ctggttgacg tgccatccgg
     5281 cagcgtgacc agggcgagcc caccccatcc cgtataggca aaggtgctgg tctggcccgc
     5341 cacgctgcga ctggccagcc atcctcgcgg ggtataggta taagtcagcg cggtgccatt
     5401 ggggtccgtt gccgccagga ggcggccggc cttgtcgtac tgggtaaagg tccacacgtg
     5461 gcccagtgcg ttcgtaatgc tgcgcagatc gccttgagtc cagttggcgg tggtgtcggt
     5521 gtaataaacg tacttggtca cgtccgcgag gtctgtgcgg ggaccgtctt cggtcagcac
     5581 ctgtccgaac gcgttatagg tataggccca gatccgtgcc ggcccatcgg cggacgcacc
     5641 gaagccttgc gcgccagtcg cgtcagtggt ggcctgttcg atgcgcttgc acagcactgc
     5701 aatgacttga ccatccggca gcttggccgt ggcgggcgcg catgaggcca gcgcgttgcc
     5761 attggtggga tcgggttgcc cgttatagac ccaggtggtg acgcgcttgg gttcggcctg
     5821 ccgggtttcg agacgccaga acggatgcca ctgcgtggtg gttttgcgct gtgtggagga
     5881 aggcgccgaa gacagcacat cggccggaca tgcatcggac gacgcgaaac cctcgatccg
     5941 cgaggtctcg aggttacggg aagcatcgta accatggcag gacttcatcc cgttgaaatc
     6001 cgtgcgggag atggcattac cgaaagtatc gtagtcaata ttgctgctgg atgcgttgca
     6061 gccggcgccc cccggcttcg atactccggt cggaaatatc tgggatttgt ccaggaccga
     6121 gctataggta cgttgaactc ccgatggatc ggtgacaaca gtcgacgtcg ggaaccattt
     6181 atcttcggaa tacttaaagg tgtagctact ctgatcaccc cctcttccag agtacacgac
     6241 cctattgttg cgatcatatt caacccgcga aaacagcgca ccatcctcat caacaacaga
     6301 caagatattc atccacgaag aaggagactc gacagaatat gtgtacgacc tcgattttcc
     6361 atcctgccaa ccaacaacag acagcgggta gccattgtaa ttaaaataca aataatttat
     6421 cactcgttgg tcagaagtcg taacagatga caccctatcc acggcatcgt agttaaactt
     6481 tatagcagcc ccattgtgat ctgttatgga agcaacattc cacgacccaa ccgcactccc
     6541 accgacatac cggatgattt cataggctcc atcgtgatga tacacacccg ccaaataccc
     6601 atcagacgta tattgctcca gcgtgttgtc ggcccgaaat agctgatagc cgcttgatgt
     6661 cttagtaaga cgatctaaaa gcccatgctc cggaacaagt tgactcgtat aaaaatatcg
     6721 cgaacgcccg gactcatcta ccgacactac actgacgtcc ttctcaattt cctgaaagaa
     6781 aagtctgcga tgccagtttg tgatccagga gaccattcct gcaggctgta tccgaaagaa
     6841 cgatcggtac gttctctcca gatatagatg gggatttgag gagtgacgaa agtcaagctc
     6901 tctaaatact tttacaccac tagcgggctc gactggatac ccaatctcat tgcaaaccgg
     6961 cggcctaacg ggcgttgtcg gaccggtaca atactttggc ggatttggtt ctcccaacgg
     7021 atatgtaccg gggtatggat gattagggca aacagggtat aaaaactccc ccgaatttcc
     7081 aagatctcca ttgtggtggt gaagaccacc ataatcgtaa tgatcatagg atgccgagca
     7141 ataccaagcg gaaaaattcg gcgtatcgta aggaagcacc tcaccaagtc taaaatcgct
     7201 gacgctgtaa ttgtgattag gttccggtat aatcctaaga gaggacaaca ccattgtaat
     7261 cggtcgctcc ggaggatttc cagcagcctc aatgaatatt tgctggcaaa tctcttgagg
     7321 accacccgcc gtaatccagc catctttcca tggcgtcgtg acaatcggca cggtcggctc
     7381 catgtaagca acttgcgcaa acacgtacga cgacgcgcaa acgacatacg ctgcaaaagc
     7441 agatctgaaa aaaaagaatt tgcacagatc tatgctttcg agcttgcgat tttcttcaaa
     7501 tttaataaac atgaataaat ttaaactacc ccaagagcaa cgcttctcca tacatatcaa
     7561 aaacaaagcc cgttaaatcg cagcatcaaa accgatacgg ttttatattc accgcgcatc
     7621 gtgccttaat cattatctcc acgcatcccc aaaactgagt agaaacgtct tcacatcagc
     7681 gtaccgtagc aaacaaggct atccacgaaa acaacctccc gctcattcgc cgcgtctccc
     7741 tacttctccc ccttcggccc ccgcgatcgc ggtttgtgct ccgacttctt cttcagcccc
     7801 agggcggcga cttcgttgga gtcggggc
//
LOCUS       DAOQEW010000179         7809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3260_length_7809_cov_6.291076, whole genome shotgun sequence.
ACCESSION   DAOQEW010000179 DAOQEW010000000
VERSION     DAOQEW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_7809_cov_6.291076"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLS35_15680"
     CDS             <1..580
                     /locus_tag="PLS35_15680"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNI20164.1"
                     /translation="LQLISVCDLFVDSAHRTPKYQDDGIPALRPRDVVNGKLNLEGAL
                     RVCEEEYEIQSKRYKPKSGDIVYSRELSYGWAALLPKSPRVCLSQGMCLFRPSFKLEA
                     SFLLYVLNGSIGREQATRAAVGAAHPHINLGDIKSYLIPMPPLEIQRQIIEQLNALQE
                     ETQRLESIYQQKLAALDELKKSLLHQAFTGQL"
     gene            590..1615
                     /locus_tag="PLS35_15685"
     CDS             590..1615
                     /locus_tag="PLS35_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNI20165.1"
                     /translation="MAKDLTNSAIDRQNILNNPYALAEIEKAAGIRGIPFENKTVVLK
                     EQVSAFFEVSLRTVENYLERNAEELSRNGYEVVKGNRLKKLKLAIQGLDVPETDFGNI
                     SKAPQLGVLDFRAFLNLAMLMTESERAGLLRKAILDIVIDTINRRTGGGTKYINQRDE
                     DFIQSAFIEENYRKQFTDALKDCVDMGNFKYALYTDKIYVSIFREKAQVYRRVLRLDK
                     RASVRDTFYSEVLDLIAAYEAGFADALVQDFRSKGRRLTSWEVDGLFVAFEKQAHWKP
                     LVEKARNKMASRDLAFRDALHLQLQEYVTPLQRDEFERFLGEKSKELAERLEEAKDVM
                     KRLKERE"
     gene            1615..2064
                     /locus_tag="PLS35_15690"
     CDS             1615..2064
                     /locus_tag="PLS35_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNI20166.1"
                     /translation="MAWVYLNLEQAIEIHGKTVEISGGGSLGQLDVGKLEAVLEHIQN
                     DDYYPTFDSKLTHLFFSACKFHCFADGNKRIAITLCAQMLLFNGYLRSVNAFIRESEN
                     ISYHVAAGTISKELLGEWIDAVLCGDEDNEGLKLKILTAISDNNEDV"
     gene            2064..4388
                     /locus_tag="PLS35_15695"
     CDS             2064..4388
                     /locus_tag="PLS35_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNI20167.1"
                     /translation="MNEAETRAEHIDPALRAAGWGVVEGSRVLREYPITLGRIEGLGR
                     RAKPLIADYVLVYKNTKLAVIEAKAWDEALTEGVAQAKQYAGKLAIRFTYATNGQGLY
                     GIDMETGKEGECPNYPGPDELWTRTFAKQNAWRDRFAAVPFEDKGGSHPGRYYQDIAV
                     ERVMSAIAAGKSRVLLTLATGTGKTFIAFQIAWKLFHSRWNLSREPSRRPRILFLADR
                     NILADQAYNAFSAFPEDALVRIEPDDIRKKGKVPKNGSLFFTIFQTFMSGPPKDGKPS
                     PYFGEYPPDFFDFIVIDECHRGGANDESNWRDILEYFAPAVQLGLTATPKRKDNVDTY
                     RYFGEPVYVYSLKDGINDGFLTPFKVRQISTTLDEYVYTPDDQLIEGEIEAKKLYTES
                     DFNKIIEIKEREKKRVDIFMGQINQKEKTLVFCATQDHALAVRDLINQMKTSTDPNYC
                     QRVTANDGELGEQHLRDFQDNEKTIPTILTTSQKLSTGVDARNIRNIVLMRPVNSMIE
                     FKQIIGRGTRLYDGKDYFTIYDFVKAHHHFSDPEWDGEPIAPEPKAAGKPVVPPMPSD
                     DGVHEGRAEYVRKKKAKVKLADGKARTIQHMMVTTFWHPDGTPMSAQQFLELLFGKLP
                     DFFRNEEELRAIWSAPDTRARLLQGLAEKGFGSEQMAEMQRIIDAENSDLFDVLAHVA
                     YAMAPLTREERAARAKVEIRTHFNGKQQGFLDFVLGQYVKVGVDELAQDKLSPLLKLK
                     YHNAIADAVADLGKPEEIGRVFAGFQKYLYQPQV"
     gene            4474..4860
                     /locus_tag="PLS35_15700"
     CDS             4474..4860
                     /locus_tag="PLS35_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20168.1"
                     /translation="MMNRCVPVCLSLIVTGCIGPLVPVKKIEQLPADERRAVLGLPIY
                     NESQLMGKDYSIINLVEGISCKNKTWDPAATKTDAINQAKYWAREQGADGIMNLQCDL
                     PRGTTTTYNCWESITCAGQAIKFPAK"
     gene            4929..5690
                     /locus_tag="PLS35_15705"
     CDS             4929..5690
                     /locus_tag="PLS35_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20169.1"
                     /translation="MMEKTGKYSPSGVWVAEDFRSNLIIDRYDPVFADMRASKLKHLR
                     SLNSEDAITWNVFRSLRQVNPVHWLTKLWENAFPAVALPGSLERTVVRLWESVAPPLG
                     LLEGGDEGDSEIDVMIESSSWVWFIEAKYRSDISTGTKVRPDRDQILRNLDVGTHYAG
                     VREFFFSLLIDSVDRSRVGIQKLEEYQNLDRVRGLLATHRADGLKNLKSLGVLTWRQM
                     GTVLHAASESATREDEQLYASRAHAWMVDRRLVQE"
     gene            5988..7373
                     /gene="hflX"
                     /locus_tag="PLS35_15710"
     CDS             5988..7373
                     /gene="hflX"
                     /locus_tag="PLS35_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNI20170.1"
                     /translation="MSKPSQSNAVLVAIRTPRVTAEEVDSSLQELTRLVKTLGYNVVG
                     RVTQKRSSDRYAAVLGEGKLAELALWTGGSGKIESAFGRPKNKAAAKSEADDSDVEEE
                     PDDESEEAIEAPSGPREQAQIVIVDCDLSPSQLKNLERAAGVPVLDRTGVIIEIFSRH
                     ARTRAARLQVEIARLNYLAPRLRETGGGSERQGGGVGGKGAGETSLELDKRRIRDRTK
                     ELREELAAIGDEHQTRRARREHELTVALVGYTNAGKSSLMRAMTGSEVLVADKLFATL
                     DTTIRPLYPETRPKVLLSDTVGFIKKLPHDLVASFKSTLDEAASASLLLFVVDASDPS
                     FRSQLDVTRKVLGEVGATDVPSLLVLNKRDRLGTDELAALKAEYPDAIVLSTRNKDDL
                     QALRERIMGYFESDMLDEELRIPFTAQKLVAEIRARMRILSEEYDAEGLTLRVRATPE
                     NLAAMKKKLER"
     gene            7399..7581
                     /locus_tag="PLS35_15715"
     CDS             7399..7581
                     /locus_tag="PLS35_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20171.1"
                     /translation="MKRQELEEKLPTMTQAQLIDTVKTCLDMIERLENDLSAGTRKLE
                     ELSALVKKLSDTNHAG"
BASE COUNT         1896 a         1944 c         2290 g         1679 t
ORIGIN      
        1 gcttcaactt atttcggttt gtgacctctt tgtggatagt gcccatcgaa cgccaaaata
       61 ccaggacgac ggtatcccag ctctgcgccc gcgtgacgtg gtgaacggaa aactcaattt
      121 ggaaggggca ttgcgcgttt gcgaagagga atatgagatt cagtcgaaaa ggtacaagcc
      181 taagtcggga gatatcgtgt attcgagaga gctgagttat ggctgggcgg cactgctgcc
      241 gaagtcacct cgtgtctgcc tttcccaagg aatgtgccta tttcggcctt ccttcaagct
      301 cgaagcttca tttctcctct acgtattgaa tggctcaata ggtcgtgaac aggctacgcg
      361 agcagccgta ggggcagccc atccacatat caaccttggc gacattaagt catacctgat
      421 tccaatgccg ccattagaaa tacaacggca gattattgag caattaaatg ctctccagga
      481 agaaactcaa cgcctcgaat ccatctacca gcaaaagctc gccgcgctgg acgagttgaa
      541 gaagtcgctg ctgcaccagg ccttcacagg gcaactctag gacattgcta tggccaaaga
      601 cctcaccaat tcggcgattg atcggcagaa tattctgaac aatccctatg cacttgctga
      661 gattgagaag gcggcgggta tccgggggat tccgtttgaa aataagacgg tggtgctgaa
      721 agagcaggtc tcggcgtttt tcgaagtctc tctacgcact gtagaaaatt atttggaacg
      781 caatgccgaa gaattgtctc gaaacgggta tgaggtagtg aagggtaacc ggttgaaaaa
      841 attgaagttg gctatccagg ggctggatgt tcccgaaaca gatttcggga acatctcgaa
      901 ggctccgcag ctgggcgtcc ttgattttcg tgcctttctt aatttggcca tgctgatgac
      961 ggaatccgag cgtgccggtc tgttgcgcaa ggcgattctg gacatcgtca ttgacacaat
     1021 caaccggcgc accggtggtg gtacgaaata tattaatcag cgcgacgaag attttattca
     1081 atccgccttc attgaagaga actatcgcaa gcagtttaca gacgcactga aggattgcgt
     1141 cgatatgggc aatttcaaat acgccctcta cacagacaaa atctacgtca gcattttccg
     1201 cgagaaggca caggtgtatc gacgtgtgct tcgattggat aagcgcgcca gtgtgcggga
     1261 cacattttac tcggaggtgc tggatttaat cgctgcgtac gaagccggat tcgcagatgc
     1321 tttggtacag gatttcagat ccaaggggcg caggctcacc tcgtgggaag tggatggttt
     1381 gtttgtggcg tttgaaaaac aggcgcattg gaaaccattg gttgagaaag cgcgcaataa
     1441 aatggctagt cgcgatctgg cgtttcgcga cgccttgcat ttacagctgc aggaatatgt
     1501 cactcccctc cagcgtgatg aattcgaacg gtttcttggc gagaagagta aggaattggc
     1561 tgaacgtctt gaagaggcca aagatgtgat gaaacgactc aaagagcgag aataatggcc
     1621 tgggtgtatc tgaatttgga gcaagcgatt gagatccacg gcaagaccgt cgagataagc
     1681 gggggtggtt cgttaggtca gcttgacgtc ggcaagttgg aggctgtgct cgaacacatt
     1741 cagaatgacg attactatcc gacgtttgat tccaagctca cgcatctatt cttcagtgcc
     1801 tgcaagtttc attgttttgc ggatggaaac aaacgcattg ccattaccct gtgtgcccaa
     1861 atgttgctct tcaatggata tttgcgaagc gttaacgcct tcattcggga gtctgaaaat
     1921 atcagctatc atgtggctgc tgggaccatt tcgaaagagt tgctcgggga atggatcgac
     1981 gctgtgcttt gtggtgatga ggataacgaa ggactgaagc taaaaatcct cactgcgatt
     2041 agcgacaaca acgaagacgt gtaatgaatg aagccgaaac cagagccgag cacatcgatc
     2101 ccgcgctaag ggccgcggga tggggcgtcg tcgagggcag ccgcgtgctg cgcgagtatc
     2161 ccattacact gggccgtatt gaaggcttgg gacggcgggc gaagccgctg atcgccgatt
     2221 acgtcctcgt ctataaaaac accaagctcg ccgtcattga agccaaggcc tgggatgaag
     2281 cgctgacgga aggcgtggcg caggcgaaac agtatgccgg aaaacttgcg attcggttca
     2341 cctatgccac caacggccag ggcctctacg gcatcgacat ggaaacgggc aaggaaggcg
     2401 agtgcccgaa ttatcccggt ccggatgaac tctggacgcg cacattcgcc aagcagaacg
     2461 cctggcgtga ccggttcgcc gcagtgccgt tcgaagataa gggcggctcg catccgggcc
     2521 gttactatca ggacatcgcg gttgaacgcg tgatgtcggc catcgctgcc ggaaagtcgc
     2581 gtgtgctgtt gacactggcg accggtaccg gaaagacgtt cattgccttt cagatcgcct
     2641 ggaagctgtt tcacagccga tggaacctca gccgcgagcc ctcacgccgt ccgcgcattt
     2701 tgtttctggc cgaccggaac atcctggccg atcaggccta caacgccttc tccgcctttc
     2761 ctgaagatgc gctggtgcgg atcgagccag acgatattcg taagaagggc aaggtgccga
     2821 agaatggcag cctgttcttc accatcttcc agaccttcat gagcggcccg ccgaaggacg
     2881 gcaaaccctc gccgtatttc ggtgagtacc cgccggactt cttcgacttc atcgtcatcg
     2941 acgagtgcca tcgtggcgga gccaacgatg aaagcaactg gcgggacatt ctcgaatact
     3001 tcgcgcctgc cgtgcagctc gggttgaccg cgacgccgaa gcgaaaagac aacgtggata
     3061 cctatcgcta ttttggcgag ccggtgtacg tctattcgct aaaggatggc atcaacgatg
     3121 gcttcctgac gcccttcaag gttcgacaga tctctaccac gttggacgaa tatgtctata
     3181 cgcccgatga ccagctcatc gagggtgaaa ttgaagccaa gaaactttat acggagagcg
     3241 acttcaataa gatcattgaa atcaaagaac gcgagaagaa acgtgtcgac atcttcatgg
     3301 ggcagatcaa ccagaaggaa aagacgctgg tgttctgcgc cactcaagac catgccctgg
     3361 cggtgcgcga cctcatcaat cagatgaaaa cgagcacgga cccgaactac tgccaacggg
     3421 tgacagcgaa cgacggggag ctcggtgaac agcacctgcg ggacttccaa gacaacgaaa
     3481 agaccattcc cacaatcctg accacctccc aaaagctctc gaccggagtg gatgccagga
     3541 acatccggaa catcgtgctg atgcggccgg tcaattcgat gatcgaattc aagcagatca
     3601 tcggacgcgg gacgcgactg tatgacggga aggactactt taccatctac gattttgtga
     3661 aggcgcatca ccatttcagc gatccggagt gggacggcga gccgatcgca ccggaaccaa
     3721 aagctgccgg gaaacccgtt gttcccccga tgccatcgga tgacggcgtg catgaagggc
     3781 gtgccgagta tgtgaggaag aagaaagcta aggtgaagct ggccgatggc aaggcgcgga
     3841 ccattcagca catgatggtg accactttct ggcatcccga tggcacgccg atgtcggcac
     3901 aacaattcct ggaactactc ttcggcaaac tgccggattt ttttcgcaat gaggaggaac
     3961 tccgtgcgat ctggagcgca ccggatacgc gtgccaggtt gttacagggg ctggccgaga
     4021 aagggtttgg ctctgagcaa atggcggaga tgcaacgcat catcgacgcg gagaacagcg
     4081 acctgttcga tgtgctggcg catgtggcgt atgcgatggc tccgctcacc cgtgaggaac
     4141 gggccgccag ggccaaggtg gagatccgaa cgcacttcaa cggcaagcag cagggcttcc
     4201 ttgatttcgt gctgggccag tatgtgaaag tcggggtaga cgaactcgca caggataagc
     4261 tctcaccgct gctcaagttg aagtatcaca atgccatcgc cgacgcggtc gccgatctag
     4321 ggaagccgga agagatcggg cgtgttttcg cagggttcca gaagtacctg tatcagccac
     4381 aggtgtaggc gggacgatcg ctgaaggtag gtggtgttca gttttgagcc cttccagatg
     4441 cccgagcaac ttttccaaag gagctagagc catatgatga atcgctgtgt accggtgtgt
     4501 ctttctttga tcgtaacagg ctgtatcgga ccccttgtcc ccgtgaagaa gattgagcag
     4561 cttcctgcgg acgaacggcg ggctgtcctg ggattaccca tctacaatga gagtcagttg
     4621 atgggaaaag attactcgat cataaacctc gtcgagggca tttcttgtaa gaataagacg
     4681 tgggatcctg ctgccactaa gacagatgcg atcaaccagg cgaagtattg ggcacgagag
     4741 cagggtgcgg acgggattat gaatctgcaa tgcgatctgc caagagggac gacgacgacc
     4801 tacaattgct gggagagcat tacctgtgca ggacaggcga tcaagtttcc tgcaaagtag
     4861 cctgcacgaa gtctaattac tccacaattt tcgtgtttgg gcttattggg aatgtgaggg
     4921 gtttgttaat gatggaaaag acaggaaagt actctcctag cggggtttgg gtcgccgaag
     4981 atttcagatc caatcttatc attgatcggt atgatccggt ctttgcagac atgagggcaa
     5041 gcaaactgaa gcatctcagg agccttaaca gcgaagatgc aatcacatgg aacgtattcc
     5101 gttcgctgag gcaagtgaat cctgtccatt ggcttactaa attgtgggaa aatgcattcc
     5161 cggctgtggc attgcctggt tcccttgagc ggacggtcgt gcgcttatgg gaaagcgtcg
     5221 cgccgccgct tggcctgctt gaaggtgggg acgaggggga ttctgaaatt gatgtcatga
     5281 ttgaatcatc ttcatgggtc tggtttatcg aggcaaagta ccggagcgat atttcaactg
     5341 gaacaaaagt tcgaccagat cgagatcaaa ttctcagaaa tcttgacgtc ggaacgcact
     5401 atgcaggggt aagggaattc ttcttcagtc tcctcatcga ctcggtagat agatcgaggg
     5461 tgggcattca aaagcttgaa gagtatcaga atctcgatag ggttagaggt ctgctggcga
     5521 cgcaccgggc agatggtttg aaaaacctaa agtcgttggg agttctgaca tggagacaaa
     5581 tgggcaccgt acttcacgcc gcgtccgagt ctgccacccg cgaggatgag caactttatg
     5641 cgagccgcgc gcatgcgtgg atggtggatc gaaggttagt tcaagagtag ttcacagcac
     5701 cttccgatga atcaattgct agaacacgag gcaggtcttg ccctcaaaga atcgcacttg
     5761 gcaggagtct agccttttcc ctttccctca cacctcgtgt gcctctacct ttcatcttca
     5821 ccaggccgct gatggcgaag cagagtcccg agtgcttccc tgacatgctg ggtgtgggat
     5881 atgggtcaat atctcaggtg tccgggcgcg atgtgggctg acttgaaccg gtcgtttcct
     5941 gttacaatgc cgaccctcac gaagccgtgc tcaaggaaag gcctgcgatg tcgaagccct
     6001 cacaatcgaa tgccgtgctt gtggcgatac gcaccccccg cgtgactgcg gaggaggtgg
     6061 acagttcgct gcaagagctc acccgcctgg tgaagacgct gggatataac gtcgtgggcc
     6121 gtgtgacgca aaagcggagt tccgatcggt atgcggcggt cctgggagag ggcaaactcg
     6181 ccgaactggc gctctggacc ggtgggtccg ggaaaatcga atcggcattt ggtcgcccga
     6241 agaacaaggc ggcggcgaag tccgaggcgg atgattcaga cgtcgaggaa gaaccagacg
     6301 acgaatcgga agaagccatc gaggcgccat ccggccctcg cgaacaggcg cagattgtca
     6361 tcgtggactg tgatctgtcg ccgtcccaat tgaaaaatct tgaacgtgcc gccggcgtgc
     6421 cggtgctcga tcggaccggg gttatcattg agattttcag ccggcacgcc cgaacgagag
     6481 cggcccggct gcaggtggag atcgcgcggc ttaattatct cgcgccacga ttgcgggaaa
     6541 ccggcggcgg cagcgagcgg cagggcggag gagtcggagg caagggagcg ggcgagacga
     6601 gtcttgagct cgataagcgc agaatccgtg atcgcacgaa ggaactccgg gaggaattgg
     6661 cggcgatcgg ggacgagcat cagacgcgcc gcgccaggcg ggaacacgaa ttgacggtcg
     6721 cgctggtggg atacaccaat gccgggaaat cctcgctcat gcgtgcgatg acgggcagcg
     6781 aggtgctcgt ggccgacaaa ctgttcgcca cgctcgatac cacgatccgg cccttgtatc
     6841 ctgagactcg tccgaaagtg ctcctgtccg atacggtggg attcatcaaa aagctcccgc
     6901 atgacctggt ggcgtcgttc aagtcgacgc tggatgaagc ggccagtgcg tcgctcctgc
     6961 tgtttgtcgt cgatgcctca gatccgtcct ttcgctccca actggacgtg acgcggaagg
     7021 tgttggggga agtcggcgcg acggatgtcc ccagcttgtt ggtgttgaat aaacgggatc
     7081 gtctcgggac ggatgaactc gccgccttga aggcggaata tcccgacgcg attgtgctgt
     7141 ctacgagaaa caaggacgat ctgcaggcgc tccgcgagcg cattatggga tactttgaga
     7201 gcgatatgct cgatgaggaa ttacgtattc cgtttaccgc tcaaaagctc gttgcggaga
     7261 tccgcgcccg gatgcgaatt ttgtccgaag agtatgatgc cgaggggctc acgctacggg
     7321 tgcgagcaac tcctgagaac ctggcggcga tgaaaaagaa gctcgaacga tgaggtgttg
     7381 gcaaggagcc actattctat gaagcgacag gaactggaag agaaactgcc caccatgacg
     7441 caggcccagc tcatcgacac ggtgaagacc tgcctcgata tgattgagcg gctggaaaac
     7501 gacctctctg cgggaacgcg caagctcgaa gaactgtcgg cgttggtcaa aaagttgtct
     7561 gacaccaacc acgccggctg acgccatatc tggctccctc cacctagctg ctctcacgct
     7621 caggaaaaat atgccagtgc atttctaggg ctttgcctaa ctcccatgtt gagggggttg
     7681 cgataaggtg aggtcataag aatttgcaat ggaaatatta tgcacgcatg acggaaacgt
     7741 tatgaacgcc tgatataaaa attgtaaatt cttggaagct ccgtcttcga aagaggacgg
     7801 agctttttt
//
LOCUS       DAOQEW010000180         7793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3271_length_7793_cov_7.925045, whole genome shotgun sequence.
ACCESSION   DAOQEW010000180 DAOQEW010000000
VERSION     DAOQEW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_7793_cov_7.925045"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..614
                     /locus_tag="PLS35_15720"
     CDS             27..614
                     /locus_tag="PLS35_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20172.1"
                     /translation="MTLIVRPILDKGEWSLLIPVGIGMLMLTRYVPKAAWLSRYAFAF
                     IVGVGAGLAIPRTISSFILKQIEDTVRPLLGIAPGGGVTFDYSLLNPASHLNGIIILI
                     GVVSVLFYFFFSVEHSGPGKAVARAGILFLMISFGAAFGYTVMARMSLLIGRLTDLIE
                     FSDASYGRPTLWLVLLTVATLIVLSRRGSTHPPNQ"
     gene            834..2066
                     /gene="amrB"
                     /locus_tag="PLS35_15725"
     CDS             834..2066
                     /gene="amrB"
                     /locus_tag="PLS35_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNI20173.1"
                     /translation="MTAKDPKQYPLLRNLQFSPIKEGEEQFIVLWDPTGLSKERLVLP
                     LNYFFIVQHLDGEHSVQDIGAIYLKRFGEFLMPNKVEQLLADLDTKLFLEGERTEQAK
                     QQALAAYRVAPSRSAQFAGRSYEADPAKLRAQINNFFVSKEGPEVKASEHKGKLIKAL
                     VAPTYEMKQAGPIYAWAYKELQDAQKPDFYVLIGTAHSGLEHPVAVTDKDFQTPLGLV
                     KVERAVTDRLREQIPAAFTDELAHHNEHALEFQLPFLQETLGQDQAFTIVPILTAFSA
                     DSLRDPQIREQVETFLQTLKDALAATGKSYCVVVGAELAHIGMRYGDSAPPTDFSFHR
                     CMQTDLEMLKHVENRDPEEFAKFIQKENDQRRISGFSPIYSMLRLIQAETGQVLRYDR
                     GITDQYNSTVTYASMAFF"
     gene            complement(2607..3017)
                     /locus_tag="PLS35_15730"
     CDS             complement(2607..3017)
                     /locus_tag="PLS35_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20174.1"
                     /translation="MTTLLPLPGRSLLQNLGPCLLLLPILLTACDGHRQRLMTEHYPS
                     YPDSVRWAIDKGNILRGMTEDQVYLALGSPVCKKDVVDEGRTVTVWLYPPIGRNACIT
                     SAFRVYFEQGAVTTWDRYSTPTRYTDPVGGVPLY"
     gene            complement(3079..3600)
                     /locus_tag="PLS35_15735"
     CDS             complement(3079..3600)
                     /locus_tag="PLS35_15735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNI20175.1"
                     /translation="MTMQFQKKDPRATIITPFGEIRIRFYPDDAPRHVENFINLAKMG
                     FYDDTTFHRVVPGFIIQGGDPLSKTPDRLLHGTGGPGYFLNPETNDRPHKRGAISMAK
                     MPRDGNSTRDFNDNGSQFFISLQDNGGLDRRYSIFGEIIRGIEVIDMIAKMPCDERDN
                     PLEPIRMKVTVKE"
     gene            3888..5417
                     /locus_tag="PLS35_15740"
     CDS             3888..5417
                     /locus_tag="PLS35_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI20176.1"
                     /translation="MPDTAVLNVDLADVWKERGRKNCIRTIAWGDEITVVQQTATYLE
                     VTITFFREKPDGSILPESITGYIEPTKSSGIKVSDVVRPVADNKVLKVNFVDVQQGDG
                     MVIESPDGKVLLIDGGDNQMFARYLAGRFRNTSLQQPKAIDCIIVTHGDADHFAGLSE
                     ILKSETNKVKRKQLFIQPQRIYHNGLVKRPTRMNNKAVPDTKLLGPTKTVGGTLYLTG
                     LEENLLDVDDKDMNEPFREWKQTLAAYNSRSPVSFRRLQLGDTQAFEFFNRNDLRIDV
                     LGPITTDVAGQPALRFLGEPPTGPRIGHDSLNDSDEGFTGHSASHTINGHSIVLRLSY
                     GGFSFLFSGDLNDEASRFLAREHNKGTLNLRSEVFKVPHHGSADFSGAFIQSVSPIVS
                     VVSSGDENARKEYIHPRATLMGALGKWSRVPEPLIFVTELVAFFQTEGPCRLKDDKAA
                     KKRGPFFGFSRTAYGLVKTRTDGHRFFVYTDSGNVQMKEAYAYQLDSSGVPQPAEVNR
                     A"
     gene            5565..6353
                     /locus_tag="PLS35_15745"
     CDS             5565..6353
                     /locus_tag="PLS35_15745"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNI20177.1"
                     /translation="MSDNSKIEWTDATWNPVRGCSKVSPGCTNCYAETFAERFRGVPG
                     HPFEQGFDLRLMPEKLTDPLRWRTPKMIFVNSMSDLFHEQIPDEYIINVARVMRLANW
                     HTYQVLTKRSDRMRDLLNGKLQFASSQSHIWWGVSVENQRHGLPRIEHLREAPARLRF
                     LSVEPLLEDLGTFSLKGIRWVIVGGESGPGARPMAKEWVLSIRDLCKTDHVPFFFKQW
                     GGVRKSETGRILNGKTYNEFPPRSTAPVPSAQELLSIREELEFA"
     gene            6359..7435
                     /gene="tcmP"
                     /locus_tag="PLS35_15750"
     CDS             6359..7435
                     /gene="tcmP"
                     /locus_tag="PLS35_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNI20178.1"
                     /translation="MSSDDFHGKPFDEGTVCKLHIFELYAREWFPVFLSSEKPRRKAI
                     HVFDFFAGPGTDRNNVLGSPLRLLSQLQDYQGAQGWEKVAIHAHFYDEDSSKIARLQE
                     NIASRGLRLSNVHYDIRALRFDEAFQGSDSILADTQAAKLVFIDQTGVAHVTTEVFRK
                     LVSSPTCDFLFFLSSSTLHRFRDHPAIWQKIDRPEDYYQVHRAALEYYRGLLPHGQQY
                     YLAPFSIKKGANIYGIIFGSAHPLGIDKFLEVAWRKDEFSGEADFPIDRENIRPGQML
                     LPMVEAQASKITVFENELERLLRDGGLLNEVDVMRVCFEYGVKRRHALPILTKLRKER
                     VIDVDFLVPDIQKFRCPRPIRMRK"
BASE COUNT         1761 a         2250 c         2100 g         1682 t
ORIGIN      
        1 tcgtgaagac ctatgacacg gtgatcatga ccttgatcgt gcgccccatt ctcgacaagg
       61 gggaatggtc gctgctgatt ccggtcggca tcggcatgct catgctcacg cgttatgtcc
      121 cgaaggcagc ctggctgtcg cgctatgcct tcgcgttcat cgtcggcgtc ggggcgggat
      181 tggcgattcc gcgaacgatc tcgtcgttca ttttgaaaca gatcgaggat accgtacggc
      241 cgctcctggg catcgccccc ggcggaggcg tgaccttcga ctattcgctg ctcaatccgg
      301 cgagccatct gaacggcatc atcatcctga tcggcgtcgt gtccgtgctg ttttacttct
      361 tcttctcggt ggaacattcc ggtcccggca aggccgtcgc acgcgcgggg attctctttc
      421 tgatgatttc cttcggcgcc gccttcggct acaccgtgat ggcccgcatg tcgctgttga
      481 ttggtcgcct gaccgatctg attgaattct cagacgcctc ctacggacgc cccacgctct
      541 ggctggtgct cctgaccgtc gccactctga ttgtcctcag ccgccgcggc agcacccatc
      601 cgccgaatca gtaggcgccc ggtgccgttg ttcgtccctc gtctctcgtc gttcgcaaga
      661 gcagctcggc gacgaccctg cgcgtcattt cgtccccacg agacgcttca cgagatacga
      721 aatacggtct cctggagatt ggcttcacgc ttcacgaacg acgttcctca aggctgttgc
      781 gcctgagaag tttgctccgt tacaatgccg ccactatgga atccacaccg accatgactg
      841 ccaaagatcc caagcagtat ccgctgctgc gcaatctgca attttcgccg atcaaggaag
      901 gggaggagca gttcatcgtg ctctgggacc ccaccggatt gagcaaggaa cgcctcgtcc
      961 tgccgttgaa ttatttcttc atcgtacagc acctcgacgg agagcacagc gtgcaggaca
     1021 tcggggccat ttatctgaag cgcttcggcg aattcctgat gccgaataag gtcgagcagt
     1081 tactggccga tctggacacc aaactctttc tcgaagggga gcgcaccgag caagccaagc
     1141 agcaggcgct ggctgcctat cgcgtggccc cttcccgctc agcgcagttc gccggccgca
     1201 gctatgaagc cgatcccgcg aagttgcgcg cccagatcaa caattttttc gtatcgaaag
     1261 agggaccgga ggtgaaggcc tccgaacaca agggcaaact catcaaggct ctcgtcgcgc
     1321 ccacctacga gatgaaacaa gccggaccga tctatgcctg ggcgtataag gaattgcagg
     1381 acgcccagaa gcctgatttc tatgtgctga tcggcaccgc tcactccggg ctcgagcatc
     1441 cagtagccgt caccgacaag gatttccaga caccattggg gctcgtgaag gtcgaacgcg
     1501 cggtcaccga ccgcctgcgc gaacagatcc ccgccgcttt caccgacgaa ttggcacatc
     1561 ataatgaaca tgcattggaa tttcagttgc cgttcctgca agagactctc ggccaagacc
     1621 aggcctttac gattgttccg atcctcacgg cgttttcggc ggacagtctg cgcgatccgc
     1681 agatccggga gcaggtcgag acgtttcttc agaccttaaa agacgcgctc gccgccaccg
     1741 gcaaatctta ttgcgtcgtg gtcggggcgg aactcgccca tatcggcatg cgctacggcg
     1801 actcggcccc gccgaccgac ttctccttcc atcgctgcat gcagaccgac ctcgagatgc
     1861 tcaagcatgt cgagaatcgc gacccggaag aattcgccaa gttcattcag aaagaaaacg
     1921 accagcgccg catctccggc ttctcgccga tctactcgat gctgcgtctg attcaggcgg
     1981 aaacagggca ggtgctgcgg tacgatcgcg ggatcacgga tcagtataat tcgaccgtga
     2041 cgtatgccag catggccttc ttctagggca ggctgctgaa aaaccccgcc acctgcgttc
     2101 tcagctcgac aaaatcctca acgtagccag aggctacgcc tccggttttc tctcgcctgc
     2161 ggccttgctg gacgggcttt ttgagcagcc tgcatgtgaa gcgccccatc gttctcgcgt
     2221 cgctcagctc gctcaacgta gcgcaaggct acgtaactca ctccttcgct agctgcggcc
     2281 tcgatggaca gtcgttttga ccgttcctcg ggaaacctgc gaaataaact gctgtccttc
     2341 cttctcggcc tcacactacg cctgcggctt cgccggataa ccgattgagc aaccagcacc
     2401 caatttcaac acctcagcac aattcagaat tatcgtgcga ctctcagtac acgtgtgtgc
     2461 gttcatgctc cgcagggtat ggcacaggag gggaaaaagt ccgcccagcg cggccgcagc
     2521 gagcgaaggg acgaggcgta cccttgcggt acgttgagga tcgaagcgat gccagaacaa
     2581 agctgagggg ctttttcacc ctcctgctag tagagaggga cgccgccgac aggatcagta
     2641 tacctggtcg gcgtgctata gcggtcccag gtggtgacgg cgccctgttc gaagtagaca
     2701 cgaaaggcac tggtgatgca ggcgttgcgt ccgatcggcg gatagagcca gaccgtgact
     2761 gtgcgcccct catccaccac gtccttcttg cacaccggcg agccaagcgc caggtacacc
     2821 tgatcttccg tcatgccgcg aagaatgttg cccttatcaa tcgcccagcg cacgctgtcc
     2881 ggatacgaag gatagtgctc cgtcatcaag cgctgtcgat ggccatcgca ggccgtcagc
     2941 aagatgggaa ggagcagcag gcaaggcccc aggttctgta gaagcgaccg acctggcaag
     3001 gggaggagtg ttgtcacaat aaatcaacag agctgacaga gtcgtcggga aacggccagg
     3061 cctcatagca cagcgaggtt attctttcac cgtcaccttc atgcggatcg gttccagcgg
     3121 gttgtcgcgt tcgtcacagg gcattttcgc gatcatatcg atgacctcga tgccccggat
     3181 gatttcgccg aaaatcgagt agcgacgatc gagaccgcca ttgtcctgca gcgaaatgaa
     3241 aaactgagag ccgttgtcgt tgaagtcgcg cgtgctgttg ccgtcgcgag gcatcttcgc
     3301 catcgaaatc gcaccgcgtt tatgaggccg gtcgttggtt tcaggattga gaaagtatcc
     3361 gggacctccg gtgccgtgca gcaggcggtc cggcgtcttg ctcaacggat caccgccttg
     3421 aatgatgaag cccggcacca cacgatggaa cgtggtgtcg tcataaaagc ccatcttcgc
     3481 caggttgatg aagttttcga catgacgcgg ggcatcgtcc gggtaaaaac ggatccggat
     3541 ttcaccgaag ggcgtgatga tcgtggcacg tggatctttt ttctgaaatt gcatggtcat
     3601 ggggcaaatg taacaggccg gcgattccat cggcaagagg gcggattgcg tgatttctct
     3661 cgatgaacgg cgtattctgt cgccgcaatc atccggccgc cattcacgag tcgatggcat
     3721 gagaccgagg cggccacaac tatgtgcaga ctgatcagaa tggcgttccg gcaaggccgc
     3781 aggaaggtcg gcgactgagg cgtacccttg cggtacgtcg cagggagacg aacgaccgag
     3841 aacgccgccg ggcgtcattc tgaccagcct gcagggggag agagcagatg cctgacacag
     3901 ccgtgctcaa tgtcgatctg gctgacgtgt ggaaggagcg gggccgcaaa aattgcattc
     3961 gcacgatcgc ctggggcgat gaaatcaccg tggtccagca gaccgccacc tacctcgaag
     4021 tcacgatcac cttctttcgc gaaaaacccg acggcagcat tcttcccgaa tccatcaccg
     4081 gttacatcga accgaccaaa tcttccggca tcaaggtgtc cgatgtggtc cgacccgtgg
     4141 cggacaacaa agtcttgaag gtcaacttcg tcgatgtgca acagggcgac ggtatggtca
     4201 tcgaatcgcc ggacggcaag gtcctcctca tcgacggagg agacaatcag atgttcgccc
     4261 gctacctcgc cggccgtttc cgcaacacca gtctccagca gccgaaggcc atcgactgca
     4321 tcatcgtcac ccatggcgac gcggaccact tcgccggact cagtgagatc ctcaaatccg
     4381 aaaccaacaa ggtcaaacgc aaacaactct tcatccagcc acagcgcatc taccacaacg
     4441 gcctcgtcaa acgtccgacc aggatgaaca acaaagcggt gcccgacacc aagctccttg
     4501 gaccgaccaa gacggtcggg gggacgctct acctcaccgg gctggaagag aatctgctgg
     4561 acgtcgacga caaggacatg aatgagccgt tcagagaatg gaaacagact ctggccgcct
     4621 acaacagccg cagcccggtc agcttccgcc gcttgcaact tggagatacc caggccttcg
     4681 aatttttcaa ccgcaacgac ctgcgcatcg acgtgttggg tcccatcacg acggatgtgg
     4741 cagggcagcc ggcgctgcgt tttctcggtg aaccgcccac agggccacgc atcgggcatg
     4801 actcgctcaa tgacagcgat gaagggttca ccggccattc cgcctcccac accatcaacg
     4861 gccattcgat cgtgctgcgc ctcagctacg gcggattcag ctttctgttt tcgggggacc
     4921 tgaacgacga ggccagtcgc ttcctcgccc gcgagcacaa caaaggcacc ctcaatctca
     4981 gatcagaagt cttcaaagtg ccgcaccatg gatccgctga tttttccggt gcgttcattc
     5041 aatccgtctc gccgatcgtc agcgtggtgt ccagcggaga cgaaaatgcg cgcaaagaat
     5101 atattcatcc gcgtgccacg ctcatggggg ccctgggtaa atggtcgcgt gtgccggagc
     5161 cgctcatctt cgtcaccgaa ctcgtggcct ttttccaaac cgaaggccca tgccgcctca
     5221 aggatgacaa ggctgccaag aaacgcggcc ccttcttcgg cttcagccgc accgcctatg
     5281 gcctcgtcaa aacccgcacc gacgggcatc gcttcttcgt ctacaccgac agcggcaacg
     5341 tgcagatgaa agaagcctac gcctaccaat tggacagttc aggtgtgccg caaccggctg
     5401 aagtgaaccg ggcgtaaagc gacaggggaa taatcggtag cgggcagtta tatgatgtaa
     5461 accaagcggt atgctctccg ttgaagtgat gagcgattgt cagattctag ggatacatat
     5521 taaactgctt ccagttattc ttaaacttcc aggggacccc atctgtgagt gataactcaa
     5581 agatagagtg gacagatgcg acttggaacc ccgttcgcgg ttgttcaaaa gttagcccgg
     5641 gttgcacgaa ttgctacgca gaaacgtttg cagaacgttt tcgcggagtg ccgggacatc
     5701 catttgaaca gggttttgat ttgcgcctaa tgccagagaa gctgactgat ccgttgcggt
     5761 ggcgcacgcc taaaatgatc tttgttaact cgatgagtga cttgttccat gaacaaatcc
     5821 ctgatgagta catcatcaac gtagcgcgtg tgatgcgatt ggccaactgg catacctatc
     5881 aggttttgac caagcgttcg gatcggatga gagatctgct taacggaaaa ctacaatttg
     5941 cctcgtcgca atcacatatt tggtggggtg taagcgtcga aaaccaaagg catggtctcc
     6001 cgcgcatcga acacctgcgt gaagctccag cccgcctccg atttttatcg gtagagccgc
     6061 tccttgaaga cctcgggacc ttcagtctca aggggattcg ctgggttatt gtgggtggag
     6121 aaagtgggcc tggggcaagg cctatggcga aggagtgggt actctcgatt cgcgatttgt
     6181 gcaaaacaga tcacgtccca tttttcttca aacagtgggg cggagtgagg aaaagtgaaa
     6241 ccggacgaat tctcaacgga aaaacctaca acgaatttcc ccctcgaagc accgcccctg
     6301 ttccttccgc acaagaactt ctaagcattc gagaagaact ggagttcgct taggctccat
     6361 gtccagcgat gactttcacg gcaagccctt cgacgaaggc acagtctgta agcttcatat
     6421 cttcgaactc tatgctcgcg aatggtttcc ggtcttcctt tcctcagaaa aacctcggcg
     6481 taaagctatc catgtattcg atttttttgc cgggcctgga actgaccgca ataacgtcct
     6541 cggcagccca ttgcggctat tgagccagct tcaggactat caaggggcgc agggatggga
     6601 aaaggttgct atccacgctc atttctatga tgaagattca agcaagatag ccaggcttca
     6661 agaaaacatc gctagtcgtg ggctacgact ttctaatgta cactacgata ttagggcact
     6721 gcgttttgat gaagcatttc agggctccga ctcgattcta gcggatacac aagctgccaa
     6781 gcttgttttc atcgatcaga ccggggtcgc gcatgtcacg actgaagtat ttcgcaaact
     6841 tgtcagctca ccaacgtgtg actttctttt ctttctttca tcctcgacac tccaccgttt
     6901 tcgtgatcat cccgccatct ggcagaaaat cgatcgacca gaagactatt atcaggtcca
     6961 tcgtgcagct ctggagtatt acagggggtt gcttcctcat ggccaacagt attatctcgc
     7021 accgttttca attaaaaaag gcgcaaatat ttacggcatc attttcggat ccgcccatcc
     7081 acttggaatc gataagtttc ttgaggtggc ttggcgtaag gatgaattca gcggggaagc
     7141 cgacttcccg atcgatcggg agaacatacg cccaggacag atgcttttac caatggtcga
     7201 agctcaggcg agcaagatca cggtttttga aaatgagctg gagcgattgc tgcgggacgg
     7261 aggcctatta aacgaagtcg atgtaatgcg ggtgtgtttt gagtatgggg tgaagagacg
     7321 tcatgctctg ccaattctta cgaaactgag gaaggagaga gtcatagacg tagacttcct
     7381 agtacccgac atccaaaagt ttcgttgtcc acgaccaatt cgaatgagaa agtagcaaaa
     7441 tgttgccgct gaacctccct tattcccgct ctcatccgcc tcagagttca accacattag
     7501 ccccggcaga tatcgaagcc tgaggcggct ggtgacgacg ttgctaagtc atagtgaacg
     7561 gcccggccat ggagcactgc tgtcagaata gtcaaatcgg tgtttgtcca aatgttcagg
     7621 atagttccgt tttttgctca caggcaactg cctgtagcca aggagctcac aactgcctcc
     7681 atgcccttcc ttctcactcg aacacctgct aagaagtgca atcatgacaa cccctaagct
     7741 gttcgaacca atccaagtcg gcgggctcac gctgagcaac cggatcgtca ttg
//
LOCUS       DAOQEW010000181         7647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3386_length_7647_cov_8.934932, whole genome shotgun sequence.
ACCESSION   DAOQEW010000181 DAOQEW010000000
VERSION     DAOQEW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_7647_cov_8.934932"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..293
                     /gene="rpmG"
                     /locus_tag="PLS35_15755"
     CDS             144..293
                     /gene="rpmG"
                     /locus_tag="PLS35_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNI20179.1"
                     /translation="MREIIDLACTVCKQRNYTTRKNKKNDPDRLERNKFCKFCRKHIA
                     HKEVK"
     gene            350..424
                     /locus_tag="PLS35_15760"
     tRNA            350..424
                     /locus_tag="PLS35_15760"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Trp,seq:cca)
     gene            517..711
                     /gene="secE"
                     /locus_tag="PLS35_15765"
     CDS             517..711
                     /gene="secE"
                     /locus_tag="PLS35_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNI20180.1"
                     /translation="MFQRLIASVREFIDGVRGELRKVSFPTKTETIGATTVVIVFCIL
                     MSLYLSMMDSVLGWLMRKVI"
     gene            781..1323
                     /gene="nusG"
                     /locus_tag="PLS35_15770"
     CDS             781..1323
                     /gene="nusG"
                     /locus_tag="PLS35_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNI20181.1"
                     /translation="MMSNKNWYVIHTYAGFEGRVKTSLLERANQMGLTERLGQVLVPT
                     EDVIEIKDGKRRTSRRKFFPGYVLVELEAPLADETLQMIKETPKVTGFVGGGAQPTPL
                     SSEEVDSLLKQVDAGAAGPREQVRFIKGDNVRIVDGPFLGFNGAVDDVDADHSRVKVF
                     VSIFGRSTPVELGFLQVERI"
     gene            1399..1821
                     /gene="rplK"
                     /locus_tag="PLS35_15775"
     CDS             1399..1821
                     /gene="rplK"
                     /locus_tag="PLS35_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNI20182.1"
                     /translation="MAKEVSAQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     KTQKDGDSIIPVIITVYKDRSFTFIMKTPPASDLLKKAAGIIKGSGVPHKDKVGKVSQ
                     AQVREIAQKKLSDLNAADLEGAIKIIQGTARSMGITVQ"
     gene            1866..2558
                     /gene="rplA"
                     /locus_tag="PLS35_15780"
     CDS             1866..2558
                     /gene="rplA"
                     /locus_tag="PLS35_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNI20183.1"
                     /translation="MGKKMTAAQEKIEPRFYELKEAVEAVKQAAFAKYDESVDLAIRL
                     GVDPKRSDQMVRGTTALPHGTGKKLRVLVFAKGEKEQEARQAGADYVGSDDLMEKIKG
                     GWMDFDCAISTPDLMASVGKLGKVLGPRGLMPNPKTGTVTFEVGKAVSEIRKGRVEFK
                     VEKAGIVQVPVGKVSFDVQKLYDNAQAVLESVVKAKPSSCKGRYIKSVTMSSTMGPGV
                     KLDPVALSKLWS"
     gene            2590..3096
                     /gene="rplJ"
                     /locus_tag="PLS35_15785"
                     /pseudo
     CDS             2590..3096
                     /gene="rplJ"
                     /locus_tag="PLS35_15785"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            3124..3525
                     /gene="rplL"
                     /locus_tag="PLS35_15790"
     CDS             3124..3525
                     /gene="rplL"
                     /locus_tag="PLS35_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNI20184.1"
                     /translation="MSAAAGTKLSQEELIKAIEGMSVLELADLVKGLETRFGVTAAAP
                     VAVAAAGGGGAAAAPAEEKTSFDVILASAPADKKIQVIKVVRELTSLGLKEAKDLVEG
                     APKPIKSGATKEEADTMKKKLEESGAKVEIK"
     gene            3606..7565
                     /gene="rpoB"
                     /locus_tag="PLS35_15795"
     CDS             3606..7565
                     /gene="rpoB"
                     /locus_tag="PLS35_15795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNI20185.1"
                     /translation="MSESTLSEFVERKDFSRIRTSIDIPDLIEIQKRSYEEFLQMEVE
                     PDRRKDQGLQAALASVFPITDYNNTAALEFSNYSLGTPKYDERECLEQGMTFAVPLKL
                     RVRLIVFDKEDKGPKKKVLDVREQEVYVGELPLMTERGTFLINGTERVVVSQLHRSPG
                     ASFTHDKGRTHASGKVLYSARIIPYRGSWLDFEFDARDILYVRIDRRRKMPATILLKA
                     FGYSTDDLLRMYYPVEEIRVSKGKLFRKLDPEIHHGLKCSTEVMEKGGKDPIVREGAK
                     LTKVLIAKLKAAGIKEIPVTPAELVGRAVLTDLVDGGKKQALAEKNQRLTEEILEKIL
                     DSDIEEFKVIYLDTTNATLVILDTLDMERTGSKEEAMVEIYRRLRPGETPSVETARAL
                     FDNLFLSPKRYDLSPVGRLKLNKKLGLDFALEQRTLTAQDIVEVVRYLVNLKIGRGEI
                     DDIDHLGNRRVRSVGELLENQFRLGLVRMERSIKERMNLLDMETVLPHDLINAKPVVA
                     AVKEFFSSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRI
                     CPIETPEGPNIGLITSLATYARINEFGFIEAPYRKVVKGRVSDELEYLSAIEGDKYII
                     AQANSKVDGSNRLVSETVSARSAGDFITATPDKIEYMDVSPKQVVSVATALVPFLEHD
                     DANRALMGSNMQRQAVPLLKAESPLVGTGMEAVVARDSGYVVQAKREGVVESVDATRI
                     VVRADAKEGRKRNDSGLDVYEMIKFQRSNQNTCITQTPVVRIGEPVKRGQVLADGPAI
                     DHGELALGKNVLVAFMPWGGYNFEDAILLSEKLVREDAFTSIHIEEFEVEARDTKLGK
                     EDITRDIPNVGEEALRDLDESGIIRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAI
                     FGEKAGDVKDTSLTVPPGVEGIVVDVKIFSRKGLDKDERSKSIESEDHMKLQRDHQEE
                     LRIIEDEKTKKVRKLLLGKVVGRDLMDPETGDVILKKKGKLTAEILKRLPDDMVRHII
                     LSDPDEQKELEDVERRAKEQIEILQTLYDEKVGRLRRGDELPPGVIKLVKVYIAMKRK
                     IQVGDKMAGRHGNKGVVSRVLPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHL
                     GWAARALGIKVASPVFDGASEKEIKELLKKAKLSPSGQTVLMDGRTGESFSSPVTVGY
                     MYVLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASIL
                     QEFLTVKSDDVPGRSRMYEAIVKGEPFLEPGLPESFNVLVKELQSLGLDVELVKSKD"
BASE COUNT         1792 a         1829 c         2421 g         1605 t
ORIGIN      
        1 ccgcgaaggc ggcaagactg tcggctcggg cgtcgttacg gaaattctag cttagtagag
       61 ggaggcgtta agcgtcaggc gtttcgagcg cggagacaat cccactctcc ggcttcacgt
      121 tacacgcttc acgggtatac gacatgcgag aaatcattga cttggcctgt acggtctgta
      181 agcagcggaa ttatacgacc cgcaagaaca agaaaaacga tccggaccgg ctggagcgaa
      241 acaagttctg caagttttgc cgcaaacaca ttgcccataa ggaagtgaag taaggggtgt
      301 ggggcgcggg gcctcgttgc gggccccgcg gcgctcatgt tccgagcgga ggggcatggt
      361 gtcaacggca gcacatcggt ctccaaaacc gagagtctag gttcaaatcc tagtgcccct
      421 gccaaagcgg atgcttctta gtggggtgat gtgtagggtg gttggtccgt ctgggggagt
      481 ctggcagtcg aaaagttcta cgagaggatt tgaaccgtgt ttcagcgatt gattgcctct
      541 gttcgagaat ttatcgatgg tgtgcgcgga gaactgcgga aggtttcttt tccgacgaaa
      601 acggaaacga tcggtgctac cacggtcgtg attgtgttct gcattctcat gtccttgtat
      661 ctgtcgatga tggattccgt gctgggctgg ctgatgcgca aggttattta gttgcgtggt
      721 gcggctggac aggtccggca ttgcccggac gatacgatgc tgatgttcag ctgagaggag
      781 atgatgagca ataagaactg gtacgtcatc catacctatg cggggtttga gggtcgcgtg
      841 aagaccagtc tcctcgagcg cgcaaatcaa atggggctca ccgagcgact tgggcaggtc
      901 ctggttccta cggaagatgt catcgaaatc aaggatggca agcgacggac ctcccggcga
      961 aaattttttc cgggctatgt cctcgtcgag ctggaagcgc cgttggctga cgaaaccttg
     1021 cagatgatca aggagacccc gaaagtcacg gggtttgttg gtggaggggc acagccaacg
     1081 ccgctctcct cggaagaagt tgattctttg ttgaagcaag ttgatgccgg agcggccgga
     1141 cctcgagagc aagtccggtt cattaaaggg gacaacgttc gtatcgtgga tggtccgttc
     1201 ctgggcttca atggcgccgt cgatgatgtg gatgccgatc acagtcgcgt gaaggtgttt
     1261 gtcagtattt tcggccggtc tactccggtc gagcttggat tcttgcaggt cgaacgtatt
     1321 tgagtcactg gattggtggg agtgggctgg gagctatttc cggtcctcgc gcagtccgca
     1381 gttctcaggg agtaactcat ggccaaggaa gtatcagctc aaattaaatt gcagatcccg
     1441 gccggtaagg ccaatcctgc tccgccggtc ggtccttcgt tgggccaaca cggtgtgaac
     1501 atcatggagt tctgcaagca attcaatgcg aagacccaaa aagatgggga tagcatcatc
     1561 ccggtgatca ttacggtcta caaggaccgg agctttacct tcatcatgaa gacgcctccg
     1621 gcgtcggact tgctcaagaa ggccgcgggt atcatcaagg gatcgggcgt cccgcacaag
     1681 gataaggtgg gcaaggtcag tcaggctcag gttcgggaaa tcgcgcaaaa gaagttgtcg
     1741 gacttgaacg cggctgatct tgaaggcgcc atcaagatta ttcagggcac agcgcgcagc
     1801 atgggcatca ccgtccagta aattcgtgca gagtgaacct ccggcggatg tgaaggagca
     1861 gcagtatggg aaagaagatg accgcggctc aggagaaaat tgagcccagg ttttatgagt
     1921 tgaaggaggc ggttgaggcc gtcaagcaag cagcctttgc caagtatgat gaatccgtcg
     1981 acctggcgat ccggctgggt gtggatccca agcgatcgga tcaaatggta cgtggaacca
     2041 cggcgctccc gcatggcacc ggcaaaaagc tccgggtgct cgtattcgcc aagggtgaaa
     2101 aggagcagga agcgcggcag gccggggctg attacgtagg ctctgatgat ctgatggaaa
     2161 aaattaaggg cggatggatg gatttcgatt gtgcgatttc cacccctgat ctcatggcct
     2221 ctgtcggtaa gcttggtaag gtgcttggtc cccgtggcct catgcccaat ccgaagaccg
     2281 gcactgtgac gtttgaggtg gggaaggccg tgagcgagat tcgcaaaggc cgtgtcgagt
     2341 ttaaggtgga aaaagccggc atcgtccagg tgccggttgg caaggtgtcg ttcgatgttc
     2401 agaagctgta cgacaatgcg caggcagtgc tggagtcggt ggtcaaggcg aagccgtcat
     2461 cctgcaaggg ccggtacatc aagagtgtga cgatgtcgag cacgatgggg cctggcgtga
     2521 agctggatcc tgtggcgttg tcgaagttgt ggagttgaga acggacaggt tctcagtaag
     2581 gggagaggta tgaagaaaga agagaaggca acagcgatcg cggagttgac agagaagttt
     2641 ggccgcgccc gcttggccat tttgacggaa tgcgccggga tgcccgtcaa tcaaatgacc
     2701 gagctgcgaa ggcagttgcg cggggctaaa gccgagtact gtgtcatcaa gaataccctg
     2761 gccgtccgcg cctcgaccgg tacgatcctc gccgatgcaa aagatcactt caagggccct
     2821 accggtctgg tgatcggata cgacgatccg gttttgccgg cgaaaatctt gcgcgatttt
     2881 attcaggccg aaaagcgatc tgagaagatc aaggtgaccg tcggcgtgtt agagggcaag
     2941 ttggtgcagg ctgcagacct tgcagccatt gcgcaattgc cgaagaagga agtgttgatt
     3001 gcaatgttgc tatcggccat gcagggtccg atacgcggcg tggtctatgc gttaagtgga
     3061 gtattgagca agtttgtgcg agtcattgca gccattcagg ataaaaagaa aggggagggc
     3121 gacatgtcag cagcagcagg aaccaagttg tcacaggaag aattgatcaa ggcaatcgaa
     3181 ggcatgagcg tgttggaatt ggccgacctg gtgaagggtc tcgagactcg tttcggcgtg
     3241 acagcggcgg ccccggtggc cgtggcggca gcgggtggcg gcggtgcggc agcggctccg
     3301 gccgaagaaa agacgtcatt cgacgtgatt ttggcgagcg ctccggccga caagaagatc
     3361 caggtcatca aggtggtgcg cgagctcacc agcctcggac tcaaggaagc caaggacctg
     3421 gtcgaaggcg caccgaagcc catcaagtcc ggcgccacca aggaagaagc cgacacgatg
     3481 aagaagaagc tcgaagagag cggagctaaa gtcgaaatca agtaattgtt tcgggcgctg
     3541 cagtgtgatt gtccgttgag atggcgcgag gcgttcatct gcttatgagc ttggagggct
     3601 aacgaatgtc ggaatcgact ctttcggagt ttgtcgaacg taaggatttt tctcggattc
     3661 gcacgagtat cgatattccc gatctcatcg aaatccagaa gcgatcctac gaagagttcc
     3721 tccagatgga agtcgagccc gaccgtcgca aggatcaagg gttgcaggcg gcgttagcca
     3781 gcgtctttcc catcaccgac tacaacaaca ctgcggcgtt ggagttttca aactactcgt
     3841 tgggaactcc caagtacgat gagcgggaat gtcttgagca gggaatgacc tttgccgttc
     3901 ccttgaaact gcgtgtgcga ctgattgttt ttgataaaga agacaagggt cccaagaaaa
     3961 aggtgttgga cgttcgagag caagaggttt atgtcggcga attgcccctg atgactgaac
     4021 gcggaacgtt tctcattaac gggaccgagc gagtggtggt cagtcagttg caccgctcgc
     4081 ccggtgcgtc ctttactcat gacaaggggc gcacgcacgc cagcggcaaa gtgttgtact
     4141 ctgccagaat tattccgtat cgcggatcgt ggctcgattt tgagttcgac gcgcgcgata
     4201 tcttgtacgt ccgcatcgat cgccgacgga aaatgccggc gaccatcctc ctgaaggcct
     4261 ttgggtatag caccgacgac ttgctccgga tgtactatcc ggtcgaagaa atccgcgttt
     4321 ccaagggcaa attgtttcga aagctcgatc ctgaaatcca tcacgggctg aagtgctcga
     4381 cagaggtgat ggagaagggc ggcaaggatc cgattgtccg cgaaggagcg aagctgacga
     4441 aggttctgat tgccaaatta aaggcagcgg ggatcaagga aattcccgtg actcctgccg
     4501 agttggtggg gcgggcggtc ctgacggatt tggtcgacgg cgggaaaaag caggccctgg
     4561 cggagaagaa tcagcggctg accgaagaga ttttggagaa aattctggat agcgacatcg
     4621 aggaattcaa ggtcatctat ctggatacaa ccaatgcgac cctggtcatt ttggataccc
     4681 tcgatatgga gcgaaccggc tcgaaggagg aggcgatggt cgagatctat cgccgtctca
     4741 ggccgggaga aactccgtca gtcgaaacgg cgcgcgcgct gttcgataat ttatttttga
     4801 gtcccaagcg ttacgatttg tctcccgtag gccgcctcaa gctgaataaa aaactgggac
     4861 tcgatttcgc tctggagcaa cgcacgctga ctgcgcagga tatcgtcgaa gtcgtccgat
     4921 acctggtgaa ccttaagatc ggccgagggg aaatcgatga catcgaccac ttggggaatc
     4981 gtcgtgtgcg atcggtcggc gagttgttgg agaatcaatt ccgtctcggc cttgtgcgca
     5041 tggagcgcag cattaaagag cgcatgaacc tgttggacat ggagaccgtg ttgccccatg
     5101 acctcatcaa cgcaaagccc gtggtggcgg cggtgaaaga gttcttcagc agcagtcagc
     5161 tctctcaatt catggaccag accaatccgc tggctgaaat tacacacaag cgccgcttgt
     5221 cggcactggg gcctggcgga ttgacccgcg agcgtgccgg cttcgaagtg cgtgacgtcc
     5281 atccgtccca ctacagtcgt atttgcccga ttgaaacgcc ggaaggtccg aacatcggat
     5341 tgatcacttc tttggcgacc tatgcgcgca tcaacgagtt cggcttcatc gaagcgcctt
     5401 accgaaaggt cgtgaagggg cgtgtcagcg acgagttgga gtacctctcg gccatcgagg
     5461 gtgacaagta catcatcgcc caggccaact cgaaagtgga tggctccaat cggctcgtgt
     5521 ctgaaaccgt ttcggcgcgc tctgccggtg attttattac cgctactccc gacaagattg
     5581 aatacatgga cgtgtccccg aagcaggtcg tcagcgtcgc gacggcattg gtgccgttcc
     5641 ttgagcacga cgacgcgaac cgcgcattga tgggatcgaa catgcagcgc caggccgtgc
     5701 cgctgctcaa ggctgagtct ccgttagttg gaaccggcat ggaagctgtg gtcgcgcgcg
     5761 attccggata cgtcgtgcag gctaagcgtg aaggtgtcgt ggaaagtgtt gatgcgacgc
     5821 gcattgtggt gcgtgccgat gccaaggaag gtcgcaagcg gaacgattca ggacttgatg
     5881 tgtatgaaat gatcaagttc cagcggtcga atcaaaatac gtgcatcacc cagacgcctg
     5941 tggtccgcat cggtgagccg gtcaagcgag ggcaagtact ggccgacggt ccggcgatcg
     6001 atcacggtga attggcgctg ggtaagaatg tcctcgtcgc gttcatgccg tggggcggct
     6061 ataacttcga agacgcgatt ctactcagtg aaaaactcgt gcgggaagat gcgttcacct
     6121 ccatccatat cgaagagttc gaggtagagg cgcgcgacac caagctgggt aaggaagata
     6181 tcacgcgcga cattccaaat gtcggagagg aagcgcttcg cgatttggat gagagcggca
     6241 ttatccgcat cggcgccgaa gtaaagcctg gcgacatttt ggtcggcaag gtcacgccga
     6301 agggcgaaac ccagttaacg ccggaagaga aattgctgcg ggcgatcttc ggcgaaaagg
     6361 ccggcgacgt caaggatacc tctctcacgg ttcctcccgg tgtggaggga atcgtcgtcg
     6421 atgtgaagat tttctcccgc aagggcctgg ataaggacga gcgctcgaag agcatcgaga
     6481 gcgaagatca catgaagctg cagcgcgacc atcaggaaga gctgcgcatc attgaggatg
     6541 aaaagacgaa gaaggttcgc aagctcctgc tcggaaaagt ggtcggtcga gatctgatgg
     6601 accccgagac gggcgatgtc atcctgaaga aaaaagggaa gctcactgcg gagatcctga
     6661 agcgtttgcc ggatgatatg gtgcgccaca tcatcctcag cgatccggat gagcagaagg
     6721 aattggaaga cgtcgagcga cgtgccaagg agcagatcga gattttgcag accttgtacg
     6781 atgaaaaagt cggtcggctg agacggggag acgagcttcc gcctggtgtg atcaagttgg
     6841 tgaaggtcta catcgcgatg aagcggaaga tccaagtcgg cgacaaaatg gcgggtcgcc
     6901 acggcaacaa aggcgtcgtg tcacgcgtat tgcctgaaga ggatatgccc tatctgccgg
     6961 atggtacgcc ggtagaaatc gtgttgaatc cgctgggcgt gccgtctcgt atgaacgtgg
     7021 gtcaaatcct ggagacccat cttggttggg cggctcgggc attgggaatt aaagtagcca
     7081 gcccagtgtt cgacggcgcg tcagagaagg agattaagga gcttctcaag aaagcgaagc
     7141 tgtcgccaag cggtcaaacc gtgttgatgg acggccgaac cggcgaatcg ttcagcagtc
     7201 cggtcacggt gggatatatg tatgtgttga agcttcacca cttggtggat gacaagatcc
     7261 acgcacgctc catcggtccc tattcgttgg tgactcaaca accgttgggt ggtaaggccc
     7321 aattcggtgg tcaacgattg ggagagatgg aagtgtgggc cttgcaggcc tatggggccg
     7381 cctccattct ccaggaattc ctgacggtga aatcagacga tgtccccggc cggtcgcgca
     7441 tgtatgaggc gattgtgaag ggagagccgt tcctggaacc tggattgccg gagtcgttca
     7501 acgtgctggt caaagagctg caaagtcttg ggctcgacgt agagctagtt aaatcaaagg
     7561 actgattcta gtgctgagtc ttgagagcaa cgatcggata gctcaccact cagaactgcg
     7621 ctcaggcgac cgttaaggag ggatcaa
//
LOCUS       DAOQEW010000182         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3428_length_7578_cov_9.366875, whole genome shotgun sequence.
ACCESSION   DAOQEW010000182 DAOQEW010000000
VERSION     DAOQEW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_7578_cov_9.366875"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..914)
                     /locus_tag="PLS35_15800"
     CDS             complement(234..914)
                     /locus_tag="PLS35_15800"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNI20186.1"
                     /translation="MTHPPSPPTDTCILKVVVGSHAHGLARPDSDKDFRSVFVLPTAE
                     LLRIGFKYQGTRMMKEEDDETAWEIGHFLQLALQGHPLVLETLVAPVVAMDDWGAELR
                     QLFPRLWSAQTAYDAFLNYCDNQRKKMLEKKDSRPEKYAAAYVRVLFNLCELLERGTF
                     SIRISETPVGEAVVKIKAGQFRPGEVIDMGEHWRAEATRRLHTCSQKAHPEIADAFLL
                     RVRKAFLT"
     gene            1086..1304
                     /locus_tag="PLS35_15805"
     CDS             1086..1304
                     /locus_tag="PLS35_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20187.1"
                     /translation="MTTHETDIVRDGCLRCGGFMVPEEIREVNQMGWRCVMCGEHIDS
                     LILEHRRKMATPEGARQLLEKRDKARLN"
     gene            2036..2377
                     /locus_tag="PLS35_15810"
     CDS             2036..2377
                     /locus_tag="PLS35_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20188.1"
                     /translation="MATVLLSGVAPVMAEGDQDWLDTMVQAVLAEQVKDGSAEEFLSP
                     YVAQLAAVRSHLLNGESEAVYRTMNRFMEMLQAREAGISAEAADRLFDYCYLVTPPRY
                     HDVSRHLHRLS"
     gene            complement(2487..2924)
                     /locus_tag="PLS35_15815"
     CDS             complement(2487..2924)
                     /locus_tag="PLS35_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20189.1"
                     /translation="MLTRLCCAGLVLALCLSTTPSRTLAESGGTGAPLKGDAARGQVL
                     FNGKGICHYCHGVDGVIDKKPSLKPETVAAIAKHAASAPDLRNRAVHTLKDNKARFRA
                     IREGHPGSGMFPDSTLSDQEITDLLAYLAALRQSASAPAKSPY"
     gene            3148..3537
                     /locus_tag="PLS35_15820"
     CDS             3148..3537
                     /locus_tag="PLS35_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20190.1"
                     /translation="MTTPKGPSKTGKIKKSTTSSSPLARKALAAKWRQKKDDVSAAME
                     GSQRQGAMETSPKISAALVRTVEAAKLRRKKEMITGAMEKSQRGTDNHGKSQSEALAR
                     TVAANERLRTKTVITAALKESQRRSGS"
     gene            3676..4344
                     /locus_tag="PLS35_15825"
     CDS             3676..4344
                     /locus_tag="PLS35_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20191.1"
                     /translation="MFEGDFQQRRTRVLIADEMALIAAGTRALLEPDCDVVGTVADGR
                     ALLSEAEILRPDIIVMEILLPLLNGLDALRPLVRKVPESKIVLLTAQESSWHVAEAFK
                     AGASGYVLKRSQPSELTQAIHAVLMGQWYLTPLIAKNVVKQDVGGEQPKRKVPTVSTL
                     TPRQREVLQLIAEGRGTKEVATLLNIAVKTVEFHKFRIMDQLNLHSTVALAKHAIIEG
                     LVRL"
     gene            4501..6486
                     /locus_tag="PLS35_15830"
     CDS             4501..6486
                     /locus_tag="PLS35_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20192.1"
                     /translation="MIPPSAAIRVCIDCLLMMALLMIAPGELHAIQIEPNPNPDANTI
                     TITPSTPRENLVPFTNVGTINIEPLASFHNANQFNNSGVINNRGTITNSGTFTEVVRP
                     FPNPGGGFLGLGEGSQFINLASGVYNGGDRTTGIGGTFVNEGAVTHGLGFIRVGETGQ
                     YIQRQAVGSFVTPTTITPDAPFTNAGRIHIAAGDFNIDPISIRAAVSRYEQASTGTTQ
                     IDARFQNIGGEVHNAGTITVGSTGEYRQEGFPGPILAGSTTNTGTFTNAGTTNIGTGV
                     FTNQGSVANSGMFTVEAGGTYSQSRGDGTGAPVASTSNSGTFANAGQVRIGEGTSFGN
                     FSNRTPPVGQYIQQASGTTQIYGTFQVGGARVENAGLMTVGTTGTYLQAFNKFVPVVS
                     TTVNSGIFTNAGSTRVDAGTFTNHGSVVNSGTFQIAVNGVATLINHGTVVNSGTFEVG
                     AGVGAVGGVSGTGSYSQTTSVAHTIVNGTFGNTISLQAGSLSGTGTITGAVVNTGGVV
                     QPGTSLVPGTLTLANYTQGIYGRLDLKIGGLLAGSQYDVLKVTGSGTFGGSLSVRLIN
                     GFTPGLGNTFSLLTCSLGCTGLNSGTLFGGGVSLPSLASGLAWLSGLTNGGNSFSLTV
                     VASAASAPEPSTLLLIASGFVYLVIWQWRQLRSVHRS"
     gene            6749..7549
                     /locus_tag="PLS35_15835"
     CDS             6749..7549
                     /locus_tag="PLS35_15835"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNI20193.1"
                     /translation="MNRCNWKKLSLSLMCVAILGLTAPLAQTTAHAVPLYGFTGHDVP
                     FSASPVISATVNYAVLPPFDPFGSMLSQLYVPAAGNPGFDSSKYTYLYQIANPASSHA
                     IWGSFQPQMSVVTPVSATSTGSFASGDLRLDFLNGGSLVNASGNNLQSTHSFGIGART
                     VSGAERIEILSNPLGGRSHPYSTFLNQGLTPGFTSQLFGFQSSNGPTFTTGLLQGDIF
                     APGSSFAAPVSLFATVPTAGAPEPGTMLLIGTGALGLLAWRWKKPSEP"
BASE COUNT         1624 a         2001 c         2248 g         1705 t
ORIGIN      
        1 agttctgaag ggacgcgctt ccatctcggt gccagtatga ggcacggctc ccatggagcc
       61 cgatgaacgg gaggcgcgga acatccggta tgagagttgt gccgggatgt tcagatggat
      121 ctcgctcggt cggcaggcct gatctctaca cggaagcggg ttcttttttg gcggggcagg
      181 ggtacgtccc gattgcccgg atgtctagga tctgaggggg agaggttgtg gatttatgtg
      241 aggaacgctt tgcgaaccct gaggaggaag gcatcggcta tttcagggtg ggccttttga
      301 gagcaggtgt gtagccggcg ggtggcttct gctctccaat gttctcccat gtcgatcact
      361 tctccggggc gaaattgccc ggccttgatt ttgaccacgg cttcaccgac cggagtttcg
      421 gagatcctga tgctgaaggt tcctcgttcc aacaactcac acagattgaa gagcacccgc
      481 acgtaggctg cggcgtattt ttcgggacgg ctgtcttttt tctccagcat cttttttcgt
      541 tgattgtcgc agtaattcag aaaggcatca taggctgtct gcgccgacca gagacgcgga
      601 aataattggc gcagctccgc tccccaatca tccatggcga cgaccggggc gaccagcgtt
      661 tccagcacca acggatggcc ttgcagcgca agttggagaa agtggccgat ctcccaggcc
      721 gtttcatcat cttcctcctt catcatcctc gtgccttgat atttgaagcc gatacgaagc
      781 aattcggcgg tgggcaatac gaagacgctg cgaaaatcct tgtcgctatc cggtctggcc
      841 aggccgtggg catgtgagcc gacgacgact ttcaggatac aggtgtcggt tgggggcgaa
      901 ggaggatgtg tcatggttgc gatgtgccaa cttgaacgag gccgccgtcc acgtcaagtg
      961 ggtttgagtg tggttcagca ttgtgggggc agggctcgac ccgtgtctgc tgaatccttt
     1021 tcatcagttc gtcctcccat gtgcagggcg accatatcac gctcaacaga aaggtgagag
     1081 catatatgac gactcatgag accgatattg tccgggacgg atgtctacgt tgcgggggat
     1141 tcatggtgcc ggaggagatt cgagaggtca accagatggg atggcggtgc gtgatgtgcg
     1201 gcgagcacat cgactcgctg attctcgaac atcggcgaaa gatggcgacg cccgaaggcg
     1261 cgaggcagct gttggagaaa cgggacaagg ccagattgaa ctagaacatc cggagggcaa
     1321 cgtcgtggcc gacattaacc ggcaagattg atccgtgcgg cccaacggat cgcgagaggg
     1381 gagatgcgac accgattgcg ccgcgaagcc gggtgcaaca attcgccttg gagatgggct
     1441 gggtccgatc ctcagcagaa gtggacaagc cgttgaggca aggagacctt gtgaacgatg
     1501 cgccagctgt cgcggtgagg ttttgaaaag aaggccaaga aaagaaataa accggcggag
     1561 gttggtccag agagggtctt tcaactcttg agatcttgga ggggtttcag gcttcccgct
     1621 tcgcacctgc gggcctgctc accacccctg cactcgatcc cgataccgtt tccgttcctc
     1681 tcaccccaac cctccgctca cttataaggt aaggctggta cggcagcggt caagccgtgg
     1741 ccctcgatga agggcccttc cacagagcac atccttcgct gtcgcgtaat agggcagccc
     1801 catgaagacc agagcgccac cccttcctat tcccatccgg tcgatggcca actccttgtg
     1861 tgtgcgtctg ttctcggtgt ccgaagggct gtgcgtgtac tggtggctgt actcggcctt
     1921 ggcataacct atgctgcgtt cagtattcgt agtgctactg gagccctcgt caaagcggga
     1981 ggtgactatg cgaatcagcc gagacataac gcggatggcg tcgttatgca tcttgatggc
     2041 gaccgttctc ctctccggtg ttgcccctgt gatggctgag ggggaccagg attggctcga
     2101 tacgatggtg caggcggtgc tcgccgagca ggtgaaagat gggagtgcgg aagaatttct
     2161 ttcgccgtat gtcgcacagc tggcggccgt gcgttcacat ctgctcaacg gcgaaagcga
     2221 agctgtgtac cggacgatga atcggtttat ggagatgctt caagcacggg aggcaggaat
     2281 ttcagcggag gcggcggatc ggctcttcga ttattgttat ctcgtcacgc cgccgcgcta
     2341 tcacgatgtc tcccggcatc tccaccggtt aagttgaagc ccgttggatt gcgagagcgg
     2401 tctgaggttg gcacaggcga aaacgtttcc gcacagagtg gtcatgtgcg atgtgcacgg
     2461 tgttccggta aggctaccaa cgacgattag tatggacttt tggcgggagc agaggcgctt
     2521 tgcctgagtg ccgcaagata agccaagagg tccgtgatct cttgatcgct tagcgtggag
     2581 tcgggaaaca tgccgcttcc cggatgccct tcacggatcg cccgaaagcg ggctttgttg
     2641 tcttttagcg tgtggaccgc acgattgcgc agatccggtg ccgaagcagc gtgcttcgca
     2701 attgcggcca cggtctctgg tttgagtgaa ggttttttgt cgatcactcc atcgacgccg
     2761 tggcagtaat ggcaaatgcc cttgccgtta aagaggacct ggcctcgcgc ggcgtcaccc
     2821 ttcaggggag cgcccgtacc tcccgactca gcgagagtcc ggctgggtgt ggtggacaaa
     2881 caaagggcca agaccaatcc agcacaacag agtcgtgtca gcatcacgtg gtgtccttag
     2941 gcagagagta aaggcaggct cagtatgccg cgtcgatagg tggaacacaa tgccgtcgac
     3001 ccacagactg tcatgcgtct tgatcgatga ggtatgaggt gtcagcgtgc cccctccacg
     3061 gaatggcttg gtatactgtg ggatatttgt ggcgcggctg tgaggctgca tgacacccac
     3121 ccagtcctga cgaggaggga aaacaccatg acgacaccaa aaggtccctc gaagaccggc
     3181 aagataaaaa agagcaccac gtcatccagt ccgttggctc gcaaagctct ggcggccaaa
     3241 tggcgacaga agaaagacga cgtgagcgca gcaatggagg gctcacaacg gcaaggggcg
     3301 atggaaacct cgccgaaaat ttcagccgcg ttggtgcgga ccgtggaagc ggcgaaactc
     3361 agacggaaga aagagatgat tacaggtgcg atggagaagt cgcagcgagg gaccgataat
     3421 catggcaaat cgcagtcgga ggccttggcg cgaacggtgg cggcgaacga gcgtctgcgc
     3481 acgaaaaccg tcatcacggc ggcgttgaag gagtcacaac gtcggtcggg ttcctgaaga
     3541 ttgtcaggtg ggttttctca tcccgtggat agcagtacgt aagaggagag gcgacatctg
     3601 tgaagtccta tctgcgctat agttgcgtct gcgtccgaaa cgcttcagga tcgaatcagc
     3661 taaacgagaa gacccatgtt cgaaggtgat tttcagcaac gcaggactcg agtacttatt
     3721 gcagatgaga tggcgctcat tgcagccggc actcgcgcct tgcttgagcc ggattgcgat
     3781 gttgtgggca ctgttgccga tggacgcgcg ctcctcagcg aggcggaaat cctgcgccct
     3841 gacatcatcg tcatggagat tctccttccc ttgctcaacg gattggacgc gcttcgtccc
     3901 ctggtcagaa aggtgcctga aagtaaaatc gtcttgctga ccgcccagga aagctcctgg
     3961 cacgtggctg aggcattcaa agccggtgcc tcgggctatg ttctgaaacg ttcgcaacca
     4021 tccgaattga ctcaagctat tcatgccgta ttgatggggc agtggtatct cactccactc
     4081 attgccaaaa atgtggtgaa gcaggatgta gggggtgagc aaccaaagag gaaggttcca
     4141 acagtgtcta cgctgactcc tcgccagcgt gaagtgttgc agttgatcgc ggagggacgg
     4201 ggaacgaagg aggtcgccac gttgctcaat atcgcggtca agaccgtcga gttccacaag
     4261 ttccgcatca tggaccagtt gaatctccac tccaccgtcg cgttggcaaa acatgccatt
     4321 attgaaggac ttgttcgcct gtaggtggtg ttactcctca cctttctgtt tgtgcggcct
     4381 caagtaaact tcccagttcc cacctgtcca cattcggcgt agggtactga ccggattctg
     4441 agagtgcccg ctcgtgcgtt cagcgctgag cccgacagcc ggtccggaag gaggtcgaac
     4501 atgattcctc cctctgctgc tattcgtgtt tgtatcgact gtttgctgat gatggcgttg
     4561 ctcatgatag ctccgggcga gcttcatgcc attcagatcg aacccaatcc gaatccagac
     4621 gcaaacacca ttacgatcac tccttccacg ccgcgcgaaa atcttgtccc gttcaccaac
     4681 gttggcacga tcaatatcga gccgcttgcc tcgtttcaca atgcgaatca gttcaacaat
     4741 tccggcgtca ttaacaatag gggcaccatc actaattcgg gtacgtttac cgaagttgtt
     4801 cgtcccttcc ccaaccctgg tggtggtttc ttaggtcttg gtgaaggatc gcaattcatt
     4861 aacctggcga gtggcgtgta taacggcggc gataggacca ccgggattgg aggcaccttt
     4921 gtcaacgaag gcgccgtcac tcacggcctt ggcttcatta gagtgggcga aacaggccaa
     4981 tatattcagc ggcaggctgt gggttcgttc gtaacgccaa cgacgattac ccccgacgcg
     5041 ccatttacga acgcggggcg catccacatc gccgccggtg atttcaacat tgatccgatt
     5101 agtattcgtg cggctgtctc gcggtatgag caagcttcaa ccggcacgac tcaaatcgat
     5161 gcgaggtttc agaacatagg cggtgaggtg cacaacgcgg ggacgatcac ggtcgggtca
     5221 actggtgagt atcgtcagga aggctttcct ggtccgatcc tggccggaag cactactaac
     5281 actggcacct ttaccaatgc gggcaccaca aacattggta caggtgtctt caccaatcaa
     5341 ggcagcgtgg caaactccgg catgttcact gttgaagcgg gagggacata tagccagtcg
     5401 cggggagatg gtaccggggc cccagtcgca tcgacctcca atagcggcac ttttgccaac
     5461 gcgggccagg tgcgtatcgg tgaggggacg tcgttcggca acttctccaa tcggacacca
     5521 ccagttggtc agtatatcca gcaagccagc ggcaccacac aaatctacgg tacgtttcag
     5581 gtcggtggtg ctcgtgtgga aaacgccggg ctcatgaccg tggggacgac gggaacctac
     5641 cttcaggcat tcaacaagtt cgttccggtc gtatccacga ccgtcaacag cggcatcttc
     5701 acgaatgcgg gcagcacgcg cgtcgatgcg gggactttta ccaaccatgg cagcgtggtg
     5761 aattccggaa cgtttcagat agcggtcaat ggagtggcga cgctgatcaa tcatggcaca
     5821 gtggtaaatt ctggaacatt tgaggtcggg gctggtgtcg gggctgttgg aggagtttct
     5881 ggaaccggga gttattcgca aactacttcg gtggctcaca cgatcgtcaa tggaacattt
     5941 ggcaatacga tcagccttca agcgggtagc ctcagtggaa cgggcaccat taccggtgca
     6001 gtggtgaaca ctggaggggt agttcaaccg gggaccagtc ttgtgccggg gacgctgacg
     6061 ctggcgaact acacccaagg catttatgga agattggact tgaagatcgg tggcttgctg
     6121 gccggatctc aatacgatgt gctgaaagtc acaggttcag ggacgttcgg cggcagtctt
     6181 tccgtgcgat tgatcaatgg atttacaccg ggattgggga acacctttag tctgctcacc
     6241 tgctccctcg gctgcacggg attgaatagc ggcacgttat ttggaggcgg cgtttcgctg
     6301 ccctccctgg catcggggct tgcttggctc agcggactca cgaacggagg caacagtttt
     6361 tcgttgacgg ttgtcgcctc ggcggcatca gcacccgagc cttcgacgct gttgctcatc
     6421 gcatccggat ttgtttatct ggtcatctgg cagtggaggc agttacggtc cgtgcatagg
     6481 agttaggaga gctcaatcga gtcgtggacg ggagatacag cctgaaccac tcctttcctt
     6541 ctatgcctcc ttgctagcat gctatggcga ggaacctccc tagttcccaa acttgcgcac
     6601 tccccttaga gtgcacacag tacaaggggc gcccacagcg aaaggacgcg ttcggaagca
     6661 tggtcgatgc tgcacggggt gcattggctg cactacgcgc tgctcatgta aggctgaaat
     6721 ggatttgtgt gggaagggaa tgagattcat gaatcgatgt aattggaaga aattaagcct
     6781 gtccctcatg tgtgtggcta tcttgggttt gactgcgcca cttgctcaaa caactgccca
     6841 tgctgttccg ctgtacggat ttacaggcca tgatgtgccg ttttccgcat ctcctgtgat
     6901 atcggcgacg gtgaattatg ctgtcctgcc gccgttcgac ccgttcggca gtatgttatc
     6961 tcaactttat gtcccggccg cgggtaatcc agggtttgat tccagcaagt acacctatct
     7021 ttatcaaatt gccaatcccg cttcttccca tgcgatatgg ggtagctttc agccgcaaat
     7081 gtccgttgtg acacctgtgt ctgcaacctc gacaggatcg ttcgcgtcag gagacctgcg
     7141 ccttgatttt ctcaacggcg gctccctcgt gaatgcgagc ggcaataatc ttcagagcac
     7201 tcatagcttt ggaattggag cgcgaacagt ctcaggcgct gagcggatag aaattttgtc
     7261 caatccctta ggtggccgct cccatcccta ttcaacattc ctcaatcagg gcctgacccc
     7321 tggtttcaca agccagttat tcggctttca atctagcaac ggtcccacat ttacgaccgg
     7381 actattgcag ggagacatct tcgcaccggg aagtagcttc gcggctcctg taagtctatt
     7441 cgccaccgtt cccacggcgg gtgctcccga accgggtacg atgttgctga tcggaaccgg
     7501 cgccctcggc cttcttgctt ggcggtggaa aaaaccaagc gagccctaaa agtgttcgcc
     7561 aaaccaacat gggtcagg
//
LOCUS       DAOQEW010000183         7564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3436_length_7564_cov_8.852843, whole genome shotgun sequence.
ACCESSION   DAOQEW010000183 DAOQEW010000000
VERSION     DAOQEW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_7564_cov_8.852843"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLS35_15840"
     CDS             complement(<1..1097)
                     /locus_tag="PLS35_15840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20194.1"
                     /translation="MSGNPIRRHPIVTLGIILFVAVAALVAFRLTSGAKTDPRKNRIL
                     TVGTMSPIRQDLDVRLTYTADLIPNQLVNIFSRVDGYIAKIYVDKGDLVKANQLLVEI
                     DHTDYIHAVNQAKANLLSAKAKVVQQDAGVRNATLTLDRMQALIKDQFVSQQDLDTAL
                     VNRDAAVALQDSLRAQVQQMTVALAQAETNLAYSYIRAPFAGYIAERNLDPGAYVSGT
                     TASTSTVSRGILSVHDVDTVRTLIEVVEKDVPLVQIGQRADVRAEAYPNEVFEGRVTR
                     IVQALNRATRTMTVEVDLPNKDHRLKGGMFARVEVLVGKHLQAIQIPLDAVSRLEESQ
                     YVYVVKDGKAHQVPVELGALAENRVEVVKGLA"
     gene            complement(1087..1734)
                     /locus_tag="PLS35_15845"
     CDS             complement(1087..1734)
                     /locus_tag="PLS35_15845"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNI20195.1"
                     /translation="MKATNASSRNASHDRQASLISSAANLFAAKGFKGTTTKEIAKAA
                     GVSEALVFKYFPTKRALYTAILAEKAPLSELLSAVEEFARKRDDHRVFTLIASYRIRP
                     GADPTMLRLLLFSALEGHELADMFFGKQHRVFYDYLAGYIQTRIDEKAFRPIDPLLAA
                     RAFIGMVVHHRLLHEIFDVPLHCPHKDIVSTYVELFLKGLEPSSTDQKRGLTRVR"
     gene            complement(1872..2414)
                     /locus_tag="PLS35_15850"
     CDS             complement(1872..2414)
                     /locus_tag="PLS35_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20196.1"
                     /translation="MMLRGQTVLHSRRCLLVFTLGAVLLFPITPALAVPMENDPNGFE
                     GIPWGAAFTESDQFVKVEEAGRIQTYELTSTPRKLGPATVESMKFSTVEGKFARVMVR
                     YTGKDTHDRLLTFLQQQFGPIDHTPGQMAGGAVKFFNWQGPDSAIILRYDLRTSQGVI
                     FFESEAFRVKLNEGNLPPVP"
     gene            complement(2414..2956)
                     /locus_tag="PLS35_15855"
     CDS             complement(2414..2956)
                     /locus_tag="PLS35_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20197.1"
                     /translation="MHDAFSRWSPLRLLQRIGCVSALLLLTAGPVLAITMANDPHGYH
                     DLSWGIPLTDVPDLMVTRENTHTIDYEFRDRQPVYADIPVESVHLSTVDAQFARVTIR
                     YRGAQAHKQVLQYLERTYGVIERLPGQMMRGLNQQYTWRGTETEITLTYEANRDRGFV
                     FIESRNLAPRFQDLMSDTAE"
     gene            3101..3598
                     /locus_tag="PLS35_15860"
     CDS             3101..3598
                     /locus_tag="PLS35_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNI20198.1"
                     /translation="MVDSDVFVQALENLGVNFFTGVPDSLLGGIIEELLTRKLYTSAV
                     REDEAVAMAAGAFMAGKIPAVLMQNSGLGTSLNTLLSLNMIYRQPCILLVSWRGFEGK
                     DAPEHLVMGETMPQLLDTMKIPHRTLSEPTMAEDLRWVAQTFMKQRIPVALLIKKGII
                     KGLHP"
     gene            3595..4173
                     /locus_tag="PLS35_15865"
     CDS             3595..4173
                     /locus_tag="PLS35_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNI20199.1"
                     /translation="MRPEQGTMQSRAQAMAALLELLTDQPVIICNGFPSREAQKLADR
                     PTHFYMIGSMGNAAAIGVGVALSKPSKKVIVFDGDGNVLMGMGTLATVGALRPKNFIH
                     VVFDNEVYGSTGNQPTISNVVQLEKVAKAAGYVAVERVLDREDLVYEFKDMLKNDGPS
                     MLLVKVNEFVEDAGRIALEPPAITARFMKAIE"
     gene            4243..5337
                     /locus_tag="PLS35_15870"
     CDS             4243..5337
                     /locus_tag="PLS35_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNI20200.1"
                     /translation="MILLNPGPVNVSERVRQALLRPDICHRESEFSDMLQRIQQKLLT
                     AFVPGAESEYVAVILTGSGTAAVESAVMSCLPLGKRMLVLNNGVYGERLSNMIGLQRL
                     GVSELKSEWHVRPDPERLRLALRQHPEVHAVSMVHHETTTGLINPVKEIAEVVDSLNR
                     VFVLDSVSGLAGEPIDIAGSHIYMVAGTAGKCIQGFPGVSFVLLRKGFLDQMRKYPKR
                     SWYLTLTHYVDEQGRGIVPFTPAVQVYYAFEEALDELLEEGVTSRFHRYRRMATRIRE
                     RMAGMGVQPFLPADVQSNTITAFHLPAGLSYTTLHDQLKARGYVIYAGQGQLESKVFR
                     VANMGALTESQINGFLGAFEEVLAGAAARA"
     gene            5334..6053
                     /locus_tag="PLS35_15875"
     CDS             5334..6053
                     /locus_tag="PLS35_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HNI20201.1"
                     /translation="MKAIILAAGVGKRLWPVTQHKPKCLIEIGGQTLLSRYLESLASV
                     KIRQATIVVGYKQEMIRAAVGSQCHGVDISYLVNEEFHRGSISSLWIARQALTDDVVI
                     MDADVLFHREILRRLVASPYENCLLMDDTVKQTGEECMVVVQGDRVIALSKKVPDRYD
                     LAGEGVGFLRVRRADTAHVIASLKGYIDQDRWDMEYEDGLLQYFRDVKVGHEKIGGLP
                     WTEIDFPEDVTKAEREILPRL"
     gene            6106..7560
                     /locus_tag="PLS35_15880"
     CDS             6106..7560
                     /locus_tag="PLS35_15880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNI20202.1"
                     /translation="MSESTLQRGAELQGLSTAILLPGAGLFGDRPGRGLADVGPLTHI
                     GGLSLFQRTVLTLQRGGIRQLIVLAGPDEELLKQALARGARVTIPVRWMPVREFPLDD
                     PRTWESLAAEVRGFCLVAGVQAVFSKGLIEHLRQTVRDGEALVVTREAGSIEPAAGRR
                     NPVVAFQEGRLVSFHNQPVQQGHQVAADLVVLPASILNPPSGAAAPLTSGLAEAPGMT
                     PMRRWLERAAIEGRVRVVAAASHAGLWYREVWDQASAGHAERTLFRSLKGEAEGFVDR
                     YFNRTFSRLLTRLFLQLKCSPNTITMVATAVGLLSAVGFGLGTYTAGIVAALLFQLAA
                     IIDCCDGEVARLTFTESPFGAWLDIAMDNVVHIAIFAGIACGAYLRHADAPEAWVSLA
                     LGSAAVFGNAMAFWLVTKAQKIGATRGWNTPKQAAWSDFILKNVASRDFSVVVFLFAV
                     ADKLDWFLWMAALGSTVFWLMMVWVIRPSARARA"
BASE COUNT         1481 a         2067 c         2404 g         1612 t
ORIGIN      
        1 gcaagaccct tcacgacttc cacacggttc tcggcgagcg cgcccagttc aacgggaacc
       61 tgatgagctt tcccgtcctt cacgacatag acatactggg attcttcgag ccggctcacg
      121 gcgtccagcg gaatctgtat cgcctgcaaa tgcttcccga ccagaacttc gacgcgggcg
      181 aacatgccgc ctttgagacg atgatcctta ttcggcagat ccacttccac ggtcatggtg
      241 cgggtggcgc ggttcaaggc ctgaacaatg cgggtgaccc ggccttcaaa aacctcgttg
      301 gggtaggctt cggcgcgaac gtcggcccgt tgcccgattt gcacaagagg cacgtccttc
      361 tccacgactt caatcaacgt acgcaccgtg tcgacgtcat gcacactcaa gatgccgcgc
      421 gagacggtgg aggtgctggc agtggtgccg ctgacgtacg cgccggggtc aaggttgcgt
      481 tcggcaatat agcccgcaaa gggcgcgcgg atataggaat aggccaggtt ggtttcggct
      541 tgtgccaggg cgacggtcat ctgttgcacc tgagcgcgca acgagtcctg taacgccact
      601 gctgcatccc ggttcaccag ggcggtgtcg agatcctgtt gggagacgaa ctggtcttta
      661 atcagcgctt gcatgcggtc gagtgtcagc gtggcgttgc gtacccccgc atcttgctgg
      721 accaccttgg ctttagccga caagagattc gctttggcct gattgacggc atgaatgtaa
      781 tcggtatgat cgatctcgac cagcaactga ttggccttta ccagatcacc tttatcgaca
      841 tagatcttgg caatatagcc gtccacgcgg gagaaaatgt tcacgagctg gtttggaatc
      901 aaatcggcgg tataggtcag ccgtacatcg agatcttgcc tgatgggcga catggtgccc
      961 acggtgagga tccggttctt gcgcgggtct gtcttggcgc cgctggtaag gcggaacgcg
     1021 accagggccg ccacggcgac gaagaggatg atacccaggg tgacgatcgg atgccgccga
     1081 atcgggttac cggacacgag tcaggcctcg cttctgatca gtggatgaag gctcgaggcc
     1141 ttttaaaaaa agctccacgt aggtggagac aatgtctttg tggggacagt gcaacggcac
     1201 gtcgaagatt tcatgcaaca accggtggtg aaccaccatg ccgatgaagg cgcgtgctgc
     1261 caacagggga tcgatgggac gaaaggcttt ttcgtcgatt ctggtctgga tatacccggc
     1321 gagatagtcg taaaacaccc ggtgttgctt cccgaaaaac atatccgcca gctcatggcc
     1381 ctccagcgca ctgaagagca acaggcgcaa catcgtggga tcggctcccg ggcggatgcg
     1441 atagctggcg atcaacgtaa agacccgatg gtcgtcacgt ttgcgcgcaa attcctccac
     1501 ggcgctcagc agttcgctta agggagcctt ttcggccagg atggcggtgt agagggctcg
     1561 tttggtcgga aagtacttaa agaccagggc ttcgctcacg ccggcggcct tggcgatttc
     1621 cttggtggtg gtgcctttaa accctttggc ggcaaacagg ttcgcggcgg aggaaatcag
     1681 gctggcctgg cgatcgtggc tggcattgcg ggagctggcg ttcgtcgctt tcacgtcgtc
     1741 gttcgtccgg tgagtgagtg gttactcacc cctcaaggta tcatgcaaga acgaagtgtg
     1801 acaaggatcg cgaatattcc gtccaccgcg cggagagccc cgatggagga gaggaatggg
     1861 tagaggatcg attagggaac gggaggtaaa ttcccttcgt tcagcttcac gcggaacgct
     1921 tcgctctcga aaaaaatcac tccctggctc gtgcgaaggt cgtagcgaag gatgatcgcg
     1981 ctgtcggggc cctgccagtt gaaaaacttc actgcgccgc cggccatctg gccgggagtg
     2041 tgatcgatcg ggccgaattg ttgttgcagg aacgtcagca gccggtcgtg ggtgtccttg
     2101 ccggtatacc ggaccatgac gcgggcgaac ttgccttcga ccgtgctgaa cttcatcgac
     2161 tcgactgtcg ccgggcctag cttcctcggc gtggaggtca gttcatacgt ctggatgcgg
     2221 ccggcctcct ccaccttcac gaactggtcc gattcggtaa aggcggctcc ccagggaatg
     2281 ccttcgaaac cgttcggatc gttctccatg ggaacagcca gcgccggggt gatggggaac
     2341 agcagcaccg cgccaagcgt gaaaaccaac aggcagcggc gtgagtgaag gacggtctgt
     2401 ccgcgcagca tcattattcc gccgtatccg acatgagatc ttgaaagcgt ggcgccagat
     2461 tgcggctttc gataaagaca aacccgcgat cccggttggc ctcataggtc agggtgatct
     2521 ctgtttccgt tcctcgccag gtatattgct ggttgagtcc tcgcatcatc tgtccgggaa
     2581 gccgctctat cactccatag gtccgttcga gatattgcag cacctgtttg tgcgcctgcg
     2641 cgccgcgata ccggatggtg acgcgcgcaa actgcgcatc gaccgtcgag aggtgcacgc
     2701 tttcgaccgg gatgtcggca tagaccggct ggcggtcgcg aaattcatag tcgatggtat
     2761 gggtgttctc gcgggtcacc atgaggtcag gcacgtcagt gagaggaatg ccccacgaca
     2821 gatcgtggta gccgtgtgga tcgttggcca tcgtgatggc caacaccggc cccgccgtga
     2881 ggagtagcag ggcgctgacg cacccgatgc gctgcaggag gcgaagaggc gaccatcttg
     2941 aaaacgcgtc gtgcatggct ttcacaaggt cccacttggc acgctaccat ggccgttcac
     3001 atggcggcaa ctctcggtgc ggcgttcttg tgaatgaggg accccttgga tataatgcgc
     3061 ctcttgtgcc ggggccaatc gggcgacagg agcatggttc atggtcgaca gtgacgtatt
     3121 tgtgcaggcc ctcgaaaatc tgggcgtcaa tttcttcacc ggagtgccgg attccctgct
     3181 gggcggcatc atcgaggaat tgctcacgcg caagctctat acgtctgctg tgcgtgagga
     3241 cgaagcagtc gccatggcgg ccggcgcctt catggccggg aaaatccctg ccgtcctcat
     3301 gcagaactcc ggactgggca cctcgctgaa caccctgctc tcgctcaata tgatctatcg
     3361 gcagccgtgc atcctgttgg tatcctggcg cggctttgaa gggaaagacg cgccggaaca
     3421 tctggtgatg ggcgagacga tgccgcagtt gctcgacacg atgaaaattc ctcatcggac
     3481 cctatcggaa ccgaccatgg cggaggatct tcgctgggtg gctcagacct ttatgaagca
     3541 gcggattcca gtggccctcc tgatcaagaa gggcatcatc aaggggttgc acccatgagg
     3601 ccggaacagg gaacgatgca gagccgggcg caggcgatgg ccgcgctgct ggaactgctg
     3661 acggatcagc ccgtcatcat ctgcaacgga tttccttcga gggaagccca gaagcttgcc
     3721 gatcggccga cgcatttcta tatgatcggc tcgatgggca atgccgccgc catcggcgtg
     3781 ggcgtggcgc tctccaaacc gtccaagaaa gtgatcgtgt tcgatggaga tggcaacgta
     3841 ttgatgggga tgggtaccct tgccaccgtg ggcgcattgc ggccgaagaa ttttatccat
     3901 gtcgtcttcg acaatgaagt gtatggctcg acaggaaatc agccgaccat ctccaatgtg
     3961 gtccaattgg aaaaagtggc gaaggcggcc gggtatgtgg cggttgaacg ggtcttggac
     4021 cgtgaagatc tcgtgtacga attcaaagat atgttgaaga acgacgggcc cagtatgttg
     4081 ctcgtcaagg tgaacgagtt tgtcgaagat gccggacgga ttgcattgga gccgccggcc
     4141 atcaccgcgc gtttcatgaa agccattgag taattgcggc gcactgaacg tgctgagtga
     4201 gcccgtcctg ctccgttccg atcaacagcc tgaggcacaa ccatgatact tctcaatcca
     4261 ggtccggtaa atgtgagcga acgggtgcgg caggcgctgc tgcgtccgga catctgccat
     4321 cgggagtccg aattttccga catgctgcag cgcattcagc agaagctgct caccgccttc
     4381 gtgcccggtg cggagtccga gtacgtggct gtcattttaa cggggtccgg cacggcggcc
     4441 gtcgagtcag cagtgatgtc ctgccttccg ttgggcaagc gcatgttggt gctcaacaat
     4501 ggggtctatg gcgaacgact gtccaatatg atcgggttgc agcgactcgg agtgtccgaa
     4561 ctcaaatcgg aatggcacgt caggcctgat ccggaacgac tccggttggc ccttcgccag
     4621 catcccgaag tgcatgcggt atcgatggtc catcatgaga cgacgaccgg tttgatcaac
     4681 ccggtgaaag aaattgcgga ggtcgtcgac agtctcaacc gggtgttcgt cctcgattcc
     4741 gtcagtggcc tcgcgggcga accgatcgat atcgccgggt cgcacatcta tatggtggcg
     4801 ggaacggcgg gcaagtgcat tcagggattc cccggcgtgt cgtttgtctt gctgcgcaag
     4861 gggttcctgg atcagatgcg gaaatatccg aagcggtcct ggtatctgac gctgacccat
     4921 tatgttgatg aacagggccg agggattgtg ccgttcacgc cggcggtgca ggtgtactac
     4981 gcgttcgagg aggcgttgga cgaattgctg gaggaggggg tgaccagccg cttccatcga
     5041 tatcgtcgca tggcgacgag gattcgcgag cggatggcgg gcatgggggt tcagccgttc
     5101 ctgccggccg acgtgcaatc gaatacgatt acggccttcc atcttcctgc gggtctgagt
     5161 tacacgaccc tgcatgatca gctcaaggcc cgcggctatg tgatctacgc cggtcaaggg
     5221 caactcgagt cgaaggtgtt ccgtgtcgct aatatgggcg ctctgaccga atcccagatt
     5281 aatggatttc tgggtgcgtt cgaggaagtt ctcgcaggag cggcggcgcg cgcatgaagg
     5341 ccatcatcct cgcggcagga gtcggcaaac gcctctggcc ggtgacccag cataagccga
     5401 aatgtctgat cgagatcggc gggcagaccc tgttgtcccg ctatctggaa tccctggcgt
     5461 cggtgaagat caggcaggcc accattgtcg tcggctacaa acaggagatg atcagggcgg
     5521 cggtaggttc acagtgccat ggcgtcgata tctcctacct cgtcaacgag gagttccatc
     5581 gcggcagcat ttcctcgtta tggattgcca ggcaggcgct gaccgatgat gtggtcatca
     5641 tggacgcgga tgtgttgttt catcgggaga tccttcggcg tcttgtggcg tccccctatg
     5701 aaaactgttt actcatggac gatacggtga agcaaacggg cgaggaatgt atggtggtgg
     5761 tgcagggtga ccgcgtcatc gccttgagca agaaggtgcc tgatcggtat gatctcgccg
     5821 gcgaaggtgt cgggttcctc cgggtgcgcc gggcggatac agcgcatgtg atcgcttccc
     5881 tcaaggggta catcgatcaa gaccggtggg acatggaata cgaggacgga ttgttgcagt
     5941 attttcgtga cgtgaaggtc gggcatgaaa aaatcggggg actgccctgg accgagatcg
     6001 attttcctga agatgtgacg aaggcggagc gggaaattct cccgagactg tagcgcacag
     6061 gcacccgcca gcggccatcc accaatagga accccattcg gaatcatgag tgaaagtacc
     6121 ttacaacgag gcgcggagct gcagggattg agcacggcca ttttactgcc tggtgccggt
     6181 ctcttcggtg atcgtccggg gcgaggtctt gccgacgtgg ggcccttgac tcacatcgga
     6241 gggttgagcc tctttcagcg gacggttctc accttgcaac ggggcggcat ccgccaattg
     6301 atcgtgttgg ccggtcccga tgaagagctg ctcaagcaag cgctggcacg aggcgcgcgc
     6361 gtgacgattc cggtccggtg gatgccggtg cgtgaatttc cgctcgacga cccgcgtacc
     6421 tgggaatccc tcgccgctga agtccggggg ttctgtctcg tggccggagt tcaggccgtg
     6481 ttttccaagg gcctcattga acatttacgg cagaccgtgc gcgacggcga ggcactggtg
     6541 gtcacgcgtg aagcgggatc gatcgaacct gcggccggtc gcaggaatcc ggtcgtcgca
     6601 tttcaggaag ggcggctggt ttcatttcac aatcagcccg tgcaacaggg acatcaggtg
     6661 gcggccgacc tggtcgtgtt gcccgccagc attttgaacc cgcccagcgg ggcggctgcc
     6721 ccgttgacga gcggcctcgc ggaagcgccc ggcatgaccc ccatgcgtcg ctggctggaa
     6781 cgggccgcca tcgaaggacg ggtgcgggtc gtggctgccg cgtcccacgc cggactctgg
     6841 tatcgcgaag tgtgggatca ggccagtgcc ggacacgctg agcgcacact gttccgttcg
     6901 ctgaaaggcg aagccgaggg ttttgtcgat cggtatttca accggacatt ttcgcggctc
     6961 ttaacgcgac tctttctcca attaaaatgc tccccgaaca ccatcacaat ggtggcgacc
     7021 gctgtcgggc tcctctccgc tgtcggattc gggttgggga cctatacggc agggatcgtt
     7081 gcagcgctgt tgtttcagct ggctgcgatc atcgactgtt gcgacggtga agtggcgcgc
     7141 ctcactttta cggaatcccc gttcggtgcc tggctggata tcgccatgga caatgtggtg
     7201 catatcgcca tctttgcagg gattgcctgc ggggcctacc ttcggcacgc cgatgcgccg
     7261 gaggcgtggg tgtcattggc gctcggaagt gcggccgtgt tcggtaatgc catggcgttc
     7321 tggctggtca cgaaggccca aaagatcggt gctacgcgag gctggaatac cccgaagcag
     7381 gctgcctggt cggacttcat tctcaaaaat gtggcgagcc gcgacttctc tgtggtcgtc
     7441 ttcctgtttg ccgtcgcgga taaactggac tggtttctct ggatggctgc actcgggtcg
     7501 accgttttct ggttgatgat ggtgtgggtg atccgaccct cggcacgggc ccgtgcttaa
     7561 agcg
//
LOCUS       DAOQEW010000184         7552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3442_length_7552_cov_8.356543, whole genome shotgun sequence.
ACCESSION   DAOQEW010000184 DAOQEW010000000
VERSION     DAOQEW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_7552_cov_8.356543"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLS35_15885"
     rRNA            complement(<1..813)
                     /locus_tag="PLS35_15885"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1136..2164
                     /locus_tag="PLS35_15890"
     CDS             1136..2164
                     /locus_tag="PLS35_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNI20203.1"
                     /translation="MENWLTIKRRSLLKITGIAAAAGLTGGCDGVGSFFGRMFAMPPR
                     ETNYFTENKKFYVVNYSDSPFNVSRDLRQDEWRLSVKGEVKNPLSLGWRELLNRENFE
                     QISTLMCIDTLPGGDSLGNARWRGISLKKLLQEAGVDEDTTRDIVFRGADAYDDSIPL
                     TRAMQDDVMLAFLMNGEKLSKEHGFPLRLLVPGLYGIKNVKWIVEIEAYAGDYKGYWQ
                     RKGWTDDGTIKTFSRIDSPGHYQTLRGPVQRFRGIAFGGPNSISRVEISLDAARTWDS
                     CEIETPMSPYSWVIWNYTWHPPKRGKYQVAVRAVDSKGQPQIAELIRPQPAGSSGLHT
                     IIADVESV"
     gene            complement(2869..3411)
                     /locus_tag="PLS35_15895"
     CDS             complement(2869..3411)
                     /locus_tag="PLS35_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20204.1"
                     /translation="MWGRAIVTNRWSLLLLLGIGFSHVTPVFGANPDSVPVADMRHIK
                     KFVAVEVQTQGTAEKLGISAAELTDVTRVTLLNKVPGIVLEASSGPSLDATERLNQLG
                     FFTCEVWTVGEQYIAAYHVDCNAGSYTPQKTPGSLWNQAILGYGPRDEVSDAVRKGVR
                     AMVELFASTFAGVRADGGGR"
     gene            complement(3690..4283)
                     /locus_tag="PLS35_15900"
     CDS             complement(3690..4283)
                     /locus_tag="PLS35_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNI20205.1"
                     /translation="MPVTARRPSRTLALHKPYDVLPCFTDPDGRPTLSRYIDVPDVYP
                     AGRLDRDSEGLLLLTSDGTLAHHITDPRHLLAKVYLVQVERIPDAAALKRLEAGVLLG
                     GKRTRPAQARLLEEAPRLPDRPVPIRFRKHIPTAWLELTLREGMNRQVRRMTAAVGHP
                     TLRLVRVAIGSITLGDLQPGEWRELTKQEVADMFHSA"
     gene            complement(4405..5025)
                     /locus_tag="PLS35_15905"
     CDS             complement(4405..5025)
                     /locus_tag="PLS35_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20206.1"
                     /translation="MWSHLSITHARRGTLFQLCSVALIALLYLDGCRPAPVATLPPTL
                     KPSLAWPECPKIGGMYELQGEALPGSMNFYRNRDSKLTLNAMLGVAAPADLAQESVRV
                     ELVHDDTLELVNRYDGAITAHQLDLQPEDRVVCEPNRIRIHRTRAEGGVAEEPLRNIS
                     EYAQELTVEGDGSLLVKTRILNQSKSAFWSTPIPPEEYAARFRRLD"
     gene            5257..6123
                     /locus_tag="PLS35_15910"
     CDS             5257..6123
                     /locus_tag="PLS35_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNI20207.1"
                     /translation="MRWQGQRESQNIEDRRGMGAARSGAGIGLGGLVLVLAVSFLTGT
                     NPLTLLNLLTGVQEITGPAEPDRPGPTGAPSDNLGKFAAVVLADTEDTWRALLPQMGS
                     AYQDPQLVLFTGAVRSACGTASSAVGPFYCPGDRKVYLDLSFFQELARRLGAPGDFAQ
                     AYVIAHEIGHHVQNLLGLAGQVTRQQQRSTPAERNALSVRMELQADCFAGVWGYHASR
                     SRQLIEPGDFEDGLHAAAAIGDDRLQQMGQGYVQPESWTHGSSEQRVAWLRRGLQSGD
                     PQVCDTFAGGRS"
     gene            complement(6189..6836)
                     /locus_tag="PLS35_15915"
     CDS             complement(6189..6836)
                     /locus_tag="PLS35_15915"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNI20208.1"
                     /translation="MQYKPGPVPEEMNVLCACAVVSGAGLGYTEVMKKILQALIIGVA
                     VVAAGCATPVIPESLEPQVDKAVTFSQIVESPDSYRGKVVVVGGEILKAKALKGGTQL
                     EVLQLPLDSDQEPVTDRMESKGRFLALQKEFLDPATIAEGTRVTVVGEVTGSSVEKMD
                     EADYRFPTLDVKHLHRWDAKRAEERSAPGPWWNVFGGVGFGGGGTRSGGGISIGF"
     gene            6873..>7552
                     /locus_tag="PLS35_15920"
     CDS             6873..>7552
                     /locus_tag="PLS35_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNI20209.1"
                     /translation="MSRSPLRYSHQLMRCAVLTVASVAFVAGLFTVSASAFDAAALDR
                     AKLATIGVLEDTQDQRTPDKPGKILVRGTGFHLRDGYIVTARHAAEKQDVTTGTVIQK
                     QIHVLTTDLHEIPADLVGDSAFMDVVVYRVAEAHRAKLLANVAFAPGDVQPGQDVFTV
                     GYPMGWGPTMSFGHLGNTNTFLQTVDTRLIQADVAACSGNSGGGLFNDKGEVVGIMHA
                     IIQTERDDS"
BASE COUNT         1688 a         2271 c         1987 g         1606 t
ORIGIN      
        1 cgtttacggc gtggactacc agggtatcta atcctgtttg ctccccacgc tttcgtgtct
       61 cagcgtcagg tatgttccag agcgccgcct tcgccaccgg ccttcctccc gatctctacg
      121 catttcaccg ctacaccggg aattccgcgc tcctctccca ccctctagcc tggcagtacc
      181 ctctgcgctt tccgggttgg gcccgaagct ttaacagaag gcttaccaaa ccgcctacac
      241 accctgtacg cccagtgaat ccgaacaacg cttgccacct tcgtattacc gcggctgctg
      301 gcacgaagtt ggccgtggct gcttctggag gtaccgtccg aacgattgct cgctccatct
      361 tccctcccga aaggggttta caatccgaag accttcatcc cccacgcggc gtcgctgcgt
      421 caggctttcg cccattgcgc aatattcctt actgctgcct cccgtaggag tctggcccgt
      481 gtcgcagtgc cagtgtggct gatcgtcctc tcagaccagc tacccgtcaa agccttggta
      541 ggccgttacc ctaccaacaa gctgatagga catgagctca tcaaagagcg ctacactcac
      601 agtgcagctt tccctccgaa cccgaagact cggaggcgta cgcggtatta gccaaccttt
      661 cggttggtta ttccccactc gatggtagat tacccatgta ttcctcaccc gttcgccgct
      721 ttacaagtgt attgctacac cttctcgctc gacttgcatg tattaggcgc gccgccagcg
      781 ttcgttctga gccaggatca aactctcata aaggttctac ttgaattgga cccgagggcc
      841 acctcttcaa tacaccttac cttactcatt tctcgctcta ttcagttgtc aaagaaccgt
      901 ttcacgcttc ggaacgtttt tcgttcccca cgcgagccgc gcactttact atgtgcgcct
      961 gaccttgtca agagggtcag gatttatttt ttttgatctg gggttcctga gaatccgtcg
     1021 caggtcttac aaccgcatcg gccactaggc tggatcaaac gtgcactctt ataccaatcc
     1081 gcatttctgg agtcaagcct tgaaaagatc tttttaatat cgcagagtat ttcttatgga
     1141 aaattggttg accataaagc ggcgaagcct cctaaagatc actggaattg cggctgcggc
     1201 cggcctcacg ggtggctgtg acggagttgg atcttttttc ggacgcatgt tcgccatgcc
     1261 ccctcgagag accaactact tcacggaaaa taagaaattc tatgtcgtga actactccga
     1321 ctccccgttt aacgtgtctc gcgacctccg acaggatgaa tggcgcctgt cggtgaaagg
     1381 cgaagtaaaa aatccgttat ccctagggtg gcgtgagtta ttaaaccgcg agaacttcga
     1441 gcaaatttcc acgctcatgt gcattgatac cctgcccgga ggagacagcc tcggaaatgc
     1501 acggtggcgc ggcatttcat tgaaaaaact attgcaggaa gccggcgtcg atgaagacac
     1561 gacgcgagac atcgtgtttc gaggagccga cgcctatgac gatagcattc cactgacccg
     1621 tgccatgcaa gatgatgtca tgctggcgtt cctaatgaac ggcgaaaaac tgtcgaaaga
     1681 gcacggattt ccgcttcgcc tgctagtgcc agggctgtat ggaatcaaaa atgtgaagtg
     1741 gattgtcgaa atcgaagcct acgccggcga ttacaagggc tactggcagc gcaagggatg
     1801 gaccgatgat ggaaccatca agacattttc tcgcatcgat tcgccaggtc attaccagac
     1861 gctacgcggt ccagtgcaac gatttcgcgg cattgctttc ggcggaccaa attcgatcag
     1921 cagagtagaa atcagtctgg acgcagcgcg aacctgggat tcctgtgaaa ttgaaacgcc
     1981 gatgtcgccc tattcgtggg tgatctggaa ttacacctgg cacccgccca aacgaggcaa
     2041 gtaccaggtc gccgttcgag ccgtagatag caaagggcag ccacagattg ccgaattgat
     2101 ccgccctcaa ccagcgggat caagcggact ccatacgatt atcgcggatg tcgaaagcgt
     2161 ctagaatcag aacccctcct aacattcgac tatcaataaa tacgtatcac cacttaccaa
     2221 ttcttgcttt cctaccaaac ctccgggcca aacccagcgc gagcctaaaa caaattcttt
     2281 cccagatcac gactgaccgg acatcaagag cgagttaggc agatcgtgcg caggaaagag
     2341 gtcgcctcat atttcttctt cgcggcacac cgtaggattt actgcgtaga acacgcgcga
     2401 gcgccatcat cacgaccaga ctccatcggc ccggagaaga ctgggagaaa gccctgatcc
     2461 aagctcactt caacatgcca tgctgcaaaa gaatacgcct cacatgtgca gacagaagag
     2521 accatattca cacacaccac ccgccccagg agtgttcact gcgtcagttg ttgcggggag
     2581 acgagtcaga ttctctcgca ttaaaattct cgctgcccgg ccgagggccg caacaggatg
     2641 cgcccagtcg gaaatgaaca gatacgaacc tcggtgtccg gctttgcatc cgagatgaat
     2701 cgttaccggc acatgtggtt gaaggagcgc cgtcagacca agagagagcg atcaataccc
     2761 gtcagagctg ctaggaagag tttctgaccc gaattgaaaa tcgaaggata tcgaggattg
     2821 attgtggggt gtatggcaga taacaacact gatgacttgt agagtcgttt agcggccccc
     2881 tccatcagct cgcacaccgg caaacgtaga cgcgaacaat tcaaccatcg ctcgcacacc
     2941 ttttctcacc gcatccgaga cttcgtccct cggtccgtag ccgaggatcg cctgattcca
     3001 caggctcccg ggggtctttt gcggcgtata ggaacctgca ttgcaatcca cgtggtaggc
     3061 ggcgatgtat tgttcaccca ccgtccagac ttcgcaggtg aaaaacccga gctgattgag
     3121 gcgctcagtc gcgtcaagag atggaccgct cgatgcttcg aggactatgc ccggcacctt
     3181 gttcagcaac gtgacgcgcg ttacgtcggt caattcagcc gcactgatac cgagcttttc
     3241 ggctgttcct tgagtctgca cctcaacagc gacaaatttt ttaatgtgcc gcatgtcggc
     3301 gaccgggacc gagtccggat tggcgccgaa gaccggcgtc acgtgtgaga agccgatgcc
     3361 cagcagcaga agcaaggacc acctgttagt cacgatggcg cgtccccaca taatcaatct
     3421 ccctcgtctg aagaaccatt caatagttcg accctattcg cctcgcttga tatacctcag
     3481 cttgacgctc catatccaga tctagatgca ttcaagagag gatgcggcat gcgtatcttc
     3541 acgcagtctt gtcagaatat tctgaaaaca acgtaggaga gtcactctca cttgtcctgg
     3601 ctctgcgaca cgttcggctc agcactgtgc caaatcaaat cgaggtggat cacgaatctc
     3661 acgtgccccg ttcacaaagc gaccgcattc taagccgaat gaaacatatc ggccacttcc
     3721 tgcttggtca gttcccgcca ttccccaggt tgcagatcac ccaaggtgat cgacccaatt
     3781 gcaacccgca ccagtcgcaa ggtcgggtgg ccaaccgccg cagtcatccg ccggacttgc
     3841 cgattcatcc cctcgcgtaa agtgagttcc aaccaagctg tgggaatatg ttttcgaaat
     3901 cgaattggaa cgggccggtc aggcaaacgc ggagcttcct cgagaaggcg ggcctgtgcc
     3961 ggccgtgtgc gcttccctcc gagcagcacg ccggcttcaa gacgctttag cgctgccgca
     4021 tcgggaatac gctcaacctg gacaaggtac acttttgcga gaagatgtcg gggatcggtg
     4081 atgtgatgcg ccagcgttcc gtcggaagtc aggaggagga gtccttcgct atcacgatcc
     4141 aatcggccgg ctggatagac atccggcaca tcaatgtaac ggctcagtgt ggggcgccca
     4201 tcaggatcgg tgaaacaagg caggacgtcg tagggcttgt gaagggcgag tgtacgtgaa
     4261 ggacggcgag ccgtcacagg catgcgctgc cctccaggac ttcagtcgta atggctctgt
     4321 aggggacaga acaggtgacg caggatgaaa tacccatgtg cacgagaggt caccgatacg
     4381 gaaggaaggt ccagactctc gcgatcagtc gagccgcctg aagcgcgccg catactcctc
     4441 aggagggatg ggggtgctcc aaaatgcaga cttgctctga ttgaggatac gggttttcac
     4501 taataagctc ccgtctcctt ccacggtcaa ttcctgggca tattcggaaa tattccgcag
     4561 cggttcctcg gccacaccac cctccgcacg ggttcgatgg atccggatcc gattaggctc
     4621 acagaccact cgatcttccg gctgcaaatc taactgatgc gcggtaatgg ctccatcata
     4681 ccgattgacc agctccagcg tgtcgtcatg caccaactcc acgcgaacag actcctgcgc
     4741 aaggtccgca ggcgcagcca ctcccaacat cgcattcagc gtgagcttac tatcgcggtt
     4801 ccgataaaaa ttcatcgacc cgggcaacgc ttctccttgc agttcataca tcccgccgat
     4861 cttcggacat tccggccacg ccaaactcgg cttcaacgtc ggtggcagag tcgccaccgg
     4921 ggctggacgg catccgtcga gatacaacaa tgcaatcagc gccaccgaac agagctgaaa
     4981 gagcgttcct cgtcgggcat gagtgatgga aagatgcgac cacattcttc aagtctagcg
     5041 aagcgctcac cggcctgtct agagagaaga aggggaaaag gccccctgtt ccgaaatgtt
     5101 cacgggctgc gccaatctga gacatccccc cgcagctcct cggctatctg gttggcaaga
     5161 gccttgccca gccatgcgaa atatgtttcc atcagctgac tcttcccaca gctaaaagac
     5221 gaccgtcggt cagtgccgca accaaggagt ccaatcatgc gatggcaagg ccagcgcgag
     5281 agccagaaca ttgaagaccg ccgaggcatg ggcgcggccc ggtccggcgc gggtatagga
     5341 ttgggcgggc ttgtgctcgt gctcgccgtc agctttctga cgggcaccaa tcccctcacg
     5401 ctcctcaatc tcctcaccgg cgtgcaggag atcaccggtc ccgcggagcc agatcgcccc
     5461 ggaccaaccg gtgcgccaag cgacaatctt gggaagtttg ccgccgtcgt cctggccgat
     5521 acggaagaca cttggcgcgc gctgctcccg caaatgggca gtgcctatca agatccgcag
     5581 ttggtgctgt tcacaggtgc tgtacggtcc gcctgcggca ccgcctcatc cgccgtgggg
     5641 cccttttact gtcccggcga ccgtaaggtc tatctggacc tctcattttt tcaggaactg
     5701 gcccgacggc ttggcgcccc cggcgacttt gcgcaggcct atgtcatcgc ccatgaaatc
     5761 ggccatcacg tgcagaatct gctcggcctc gctggacagg ttacccggca gcaacaacgc
     5821 agtactccgg ccgaacgcaa cgcgctttcc gtccgcatgg aattacaagc cgattgtttt
     5881 gcgggtgtgt ggggatacca tgccagtcgt agtcggcaac tcatcgaacc cggcgatttt
     5941 gaggacggac tgcacgccgc tgcagccatc ggtgacgatc gccttcaaca aatggggcag
     6001 ggttacgtgc aaccggaaag ctggactcac gggtcatccg agcaacgggt ggcctggctg
     6061 cggcgtggtc tgcagtcagg cgatcctcaa gtgtgcgata cctttgccgg cgggcgatct
     6121 tagaccagtg cagaaggctg gataacggag tacagagcgg aagaagagac taggggactg
     6181 ccggcgagtt aaaatccaat actgatgccg ccgccactgc gggtgccgcc cccgccgaat
     6241 cccacaccac cgaacacgtt ccaccaggga ccgggcgcgg aacgttcctc tgctcgtttc
     6301 gcatcccacc gatggagatg tttgacgtcc agcgtaggga aacgatagtc ggcttcatcc
     6361 atcttttcca ctgagctccc cgttacctct ccgacaaccg tcactctggt gccttcggca
     6421 atggtggctg gatcgagaaa ctccttttgt agggcgagaa atcgcccttt ggattccatc
     6481 cggtcggtca cgggctcctg gtcactatcc aatggcagct gcaggacttc cagctgcgtg
     6541 cctcccttca gggccttcgc cttcagaatc tctccaccaa cgacgacgac tttgccgcga
     6601 taggaatcgg gggattcaac gatctggctg aacgtcacgg ctttatcgac ctgcggttcc
     6661 agtgattccg gaatgaccgg cgtcgcgcag ccagcggcaa cgaccgccac tccaatgatc
     6721 agtgcctgaa ggatcttttt catcacctca gtatagccca gcccggcgcc tgatactacg
     6781 gcgcaggcgc acaacacatt catttcctcc ggcacgggcc ctggtttata ctgcacagac
     6841 ctgacgacat ccacacgaaa ggaatagttc gtatgtcacg ctccccgctt cgttactcac
     6901 atcagctcat gcgttgtgcg gtactgacgg ttgcctccgt tgcgttcgtc gccggcctct
     6961 tcaccgtctc ggcctcggca tttgatgcag cagcactaga tcgcgccaag ctggcgacca
     7021 tcggcgttct ggaagacacg caagatcagc gtacgcctga caaaccgggg aaaattttag
     7081 tgcgtggcac ggggtttcac ctccgtgatg ggtacatcgt gaccgcccgc catgcggcgg
     7141 aaaagcagga tgtcacaacc ggcaccgtca ttcaaaaaca gatccatgtg ttgacgaccg
     7201 acctccatga gattcccgca gacctggttg gtgacagcgc cttcatggac gtcgtcgtct
     7261 accgcgttgc tgaagcacat cgcgccaaac tcttggccaa cgtggccttt gctcccggtg
     7321 acgtgcagcc agggcaggat gtctttaccg tcgggtatcc catgggctgg ggccccacca
     7381 tgtcgttcgg ccacctgggc aatacgaaca cgttccttca gacggttgat acacggctca
     7441 ttcaagccga tgtcgccgcc tgcagcggca actccggtgg cggcctcttc aacgacaagg
     7501 gtgaagtcgt cggcatcatg catgccatca tacaaacgga acgcgacgac tc
//
LOCUS       DAOQEW010000185         7543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3456_length_7543_cov_10.425347, whole genome shotgun sequence.
ACCESSION   DAOQEW010000185 DAOQEW010000000
VERSION     DAOQEW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_7543_cov_10.425347"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2170)
                     /gene="fusA"
                     /locus_tag="PLS35_15925"
     CDS             complement(89..2170)
                     /gene="fusA"
                     /locus_tag="PLS35_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNI20210.1"
                     /translation="MARQTPLERTRNIGIMAHIDAGKTTTTERILFYTGMTHKMGEVH
                     EGAATMDWMEQERERGITITAAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVQGVEPQSETVWRQADKYEVPRIAFMNKMDRIGADFYASVQSIIDRLGANPV
                     PIQIPIGREAEFRGSVDLISMKGYFYDDETLGAKYKIGEIPADMVDQANDYRQKMLDA
                     VVEFDDQVMEKYLNGQSLAEDEVRRVVRAGTIAMKIVPVICGSAFKNKGVQQLLDAVV
                     DFLPSPVDIPPVIGVDPATGKDVERSPSDTEPFSALAFKIMTDPFAGQLTFFRVYSGT
                     LKTGTPVLNVTKGTKDRVGRLLKMHANKREEIEIVYAGDIAAAVGLKSATTGDTLADE
                     KQPVLLEIMKFPEPVIAMAIEPKTKPDQEKMGFALQKLAQEDPSFRVRTDEETAQTII
                     AGMGELHLEIIVDRLMREFKVEANVGKPEVAFRETIRRKAEAESKYIKQTGGRGQYGH
                     VVMTVEPSEPGKGLEFVNKTVGGSIPKEYIPAIEKGVKERMETGVVAGFPLRDVKVTV
                     IDGSYHDVDSNEMAFKIAASMGFADACKKADPVLLEPIMKVEVLVPQDFMGDVIGNLN
                     GRRGKVQGMKVRAGAQAIEATVPLMEMFGYATDLRSRTQGRATYSMEFDRYDQVPRQI
                     AEAITAKHRGE"
     gene            complement(2284..2757)
                     /gene="rpsG"
                     /locus_tag="PLS35_15930"
     CDS             complement(2284..2757)
                     /gene="rpsG"
                     /locus_tag="PLS35_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002006497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNI20211.1"
                     /translation="MPRGQFFGHREAQPDSKYRDKLVGKFLNVLMGGGKKSTAERICY
                     GAFDLIQEKTNGGDPMKIFKSAIDNVKPVVEVKSRRVGGASYQVPVEIRPSRRVSLAL
                     RWITEYSRSRGGKSMQDRLAAELLDASNNTGASVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2820..3191)
                     /gene="rpsL"
                     /locus_tag="PLS35_15935"
     CDS             complement(2820..3191)
                     /gene="rpsL"
                     /locus_tag="PLS35_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNI20212.1"
                     /translation="MPTINQLVRKGRTLVKSKTKSPALKRCPQKRGVCLRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGSL
                     DAVGVADRKQARSKYGAKRPK"
     gene            complement(3299..7429)
                     /gene="rpoC"
                     /locus_tag="PLS35_15940"
     CDS             complement(3299..7429)
                     /gene="rpoC"
                     /locus_tag="PLS35_15940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNI20213.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPEKDGLFCAKIFG
                     PIKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGTLLDMSLKQLEKILYFESYVMVDPGSTSMSEQELVSEEQLRSLQSEYGSGAFKV
                     GIGAEAIRELLRKVDINTQWDELHVKAKASASAALKKKYAKRLKVLEAFRRSGNKPEW
                     MIMDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLIELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRAIRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     TVIVVGPELRLHQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQVEARVLMMSINNILSPANGKPIAVPSQDMVLGCYWLTKERVGAKGEGKL
                     FGSPEEARIAYDAGALDEHARIKVRCNGTLVQTTAGRVILSEILPPMMPFADANKLMT
                     KKEMSKLIDAVYRQAGHRETVTFLDKIKDLGFHYATRAGMSICIDNMHIPSRKEDLIG
                     KAQHEVNEIEKQYSEGLITNGERYNKVIDIWAHVTEQVANEMMKELGAGGDPAKAESF
                     NPIFMMADSGARGSSQQIRQLGGMRGLMAKPSGEIIETPITANFREGLTVLQYFISTH
                     GARKGLADTALKTANSGYLTRRLVDIAQDVIVTEEDCGTTDGILVSALLEGGEIIQPL
                     EERLLGRLAGEDIRDPVTGEIIVSFNEEIDEEQAKAVVEAGVDRVKIRSVLTCQSARG
                     VCRLCYGRDLSRGRLVEKGEPVGVIAAQSIGEPGTQLTMRTFHIGGTASKVVEQTVLE
                     AKHAGHLKYMSLDAKKNADVHNAGIAVRNKEGEWVVMNRNAKIAIVDDSGREREKYPV
                     VYGAKIKLKDGDRIEVGQKLVEWDPYSLTILTETGGKVAYGDILEGVTMKEEFDEVTG
                     LSRKVIIEQSGATLRPRVSIKDDSGKTAKVSGAGAPVARYLLPVGAHIFVEKGAMVHP
                     GDVLAKIPRETTKTKDITGGLPRVAELFEARKPKEQAVISEIDGEVSYGGFVKGMRKV
                     LVDNKMGDVKEYFIPKGKHVNVHEGDWVRAGEPLMDGSANPHDILDVLGPKELQKYLV
                     DEVQDVYRLQGVSINDKHIEIIVRQMLRKVRIEDPGDTSFLPGSQVSKGMFDVENQRV
                     LEKDGKPALGKPVLLGITKAALTTDSFISAASFQETTRVLTEAAINGREDNLLGLKEN
                     VIVGRLIPAGSGFEEYRETFVASAKAPGELSGDQSQPVAVGQAPITSVGSASENNA"
BASE COUNT         1723 a         2338 c         1741 g         1741 t
ORIGIN      
        1 cgatcgtccc gatgttcacg tggggctttc gtcgctcaaa tttcgccttc gccatccccg
       61 atcctcccta gttcacacaa cccagaccct actctccacg atgcttagca gtaatcgctt
      121 ccgcgatctg ccgaggcacc tgatcgtagc ggtcaaactc catactgtat gttgcacggc
      181 cctgcgtacg ggaacgcaaa tccgtcgcat atccgaacat ttccataaga ggcacggtcg
      241 cttcgatcgc ttgagccccg gcccgaacct tcatgccctg aacttttccg cggcggccat
      301 tcaggtttcc gatgacatcg cccataaagt cctgaggcac caacacctcc accttcatga
      361 ttggctcaag aaggacagga tccgccttct tgcaagcatc ggcaaagccc atcgaggcgg
      421 caatcttaaa tgccatttcg ttcgagtcaa catcgtgata cgaaccatca atcacggtga
      481 ccttcacatc tcgcagcgga aatccggcaa caacaccggt ttccattcgc tccttcacac
      541 ccttttcgat cgccggaata tattctttcg ggatagagcc accaactgtc ttattgacga
      601 actccaatcc cttcccaggc tcggatggct caacagtcat caccacgtgg ccatactgcc
      661 ctcgaccacc ggtctgcttg atgtacttcg attcagcctc agccttccgg cgaatcgttt
      721 ctcggaatgc cacctcaggc tttccgacat tcgcctcaac cttaaattcg cgcatcaaac
      781 ggtcgacaat aatttcgaga tgcagctcac ccattccagc aataatggtc tgggcggtct
      841 cttcgtccgt gcgaacgcgg aaggatggat cttcctgggc taacttttgc agtgcaaagc
      901 ccattttttc ctgatcaggc ttagtcttag gctcgatagc catcgcaata accggctcag
      961 gaaacttcat gatttccaac aaaaccggtt gcttctcgtc cgccagcgtg tcaccggtcg
     1021 tcgcgctctt gagaccaacc gcagcagcga tatcgccggc atacacaatc tcgatctctt
     1081 cacgcttatt tgcgtgcatc ttcaagagac gaccaacccg atctttcgtc cccttcgtga
     1141 cattgagaac aggagtcccg gtctttaacg ttccggaata gacccggaag aaggtcagct
     1201 gtccagcaaa agggtccgtc ataattttga atgcgagagc ggaaaatggc tcggtatcgg
     1261 aagggctacg ctccacatct ttcccggtgg caggatccac accaataact ggaggaatat
     1321 caaccgggga cggaaggaaa tccacaactg catccaataa ttgctgcaca cccttattct
     1381 tgaaagccga accacaaata acaggaacaa tcttcatcgc aatggtgccg gcacgaacaa
     1441 cgcggcgaac ctcatcctca gccaaggact ggccgttaag atacttctcc atcacctgat
     1501 cgtcgaattc gacaacggca tcgagcattt tctgccgata gtcattggcc tgatcgacca
     1561 tatcagcagg aatttcaccg atcttatatt tcgcaccaag cgtctcgtcg tcgtaaaaat
     1621 agcccttcat cgaaatcaga tccaccgaac cgcgaaactc ggcttcacgt ccgatcggaa
     1681 tctgaatagg aacgggattc gcccccagcc gatcgatgat ggactggaca ctcgcataaa
     1741 aatccgcacc aatccgatcc atcttattca tgaaggcgat acgggggacc tcatacttat
     1801 cagcctgacg ccagacagtt tcagactgcg gctcgacacc ctgaacagaa tcgaacgccg
     1861 caaccgcacc atcgagcacg cgcagagacc gctccacctc aatcgtgaaa tcgacgtggc
     1921 ccggcgtatc aataatattg atgcgatgat cacgccaaaa acaggttgta gccgccgcgg
     1981 tgatcgtaat accacgctcg cgctcctgct ccatccagtc catggtcgca gcaccctcat
     2041 gcacttcacc catcttatgg gtcatgcccg tataaaagag gatgcgctca gtggtcgtcg
     2101 tctttccggc atcaatgtgg gccatgatgc cgatattcct tgtacgctct aacggtgtct
     2161 gcctagccac aactccccca gaaaaaacga acgtgccgcc atcagagccg gagaccattc
     2221 actgcgacac tcacacctac gagcaaatat cgcagcccgg ctcaacagca gcacaactca
     2281 acactaccaa cgataatgag caaaggcctt attggcctct gccatacggt ggacatcctc
     2341 acgcttcttc actgatgcac ccgtattatt ggatgcatcc aacaattcag ccgcaagcct
     2401 atcctgcata ctctttccgc cacgggagcg ggaatattcg gtgatccatc gcaaagccaa
     2461 cgagacacgg cgagacggcc gaatctcaac cggaacctga taggaggcac caccaacccg
     2521 acgagacttc acctccacaa ccggcttcac gttatcgatc gccgatttga agatcttcat
     2581 cggatcgccg ccgttcgtct tctcctggat cagatcgaag gctccataac agatccgctc
     2641 tgccgtgctc ttcttcccac cacccatcag gacgttcaag aacttcccga caagcttgtc
     2701 tcgatacttc gaatcaggct gcgcttctcg atgaccgaaa aattgtccgc gtggcatcgt
     2761 tgtcctataa aataaagtca gtattcattg cacgtacgcg caatgagagg atgaactatc
     2821 tacttgggcc gcttcgcccc gtacttggac cgggcctgct ttctatcagc aactcccacc
     2881 gcatccaaag aaccacgcac aatgtgataa cgaacgcccg gcaagtcctt cacacgacca
     2941 ccacgaacca ggacgatcga gtgctcctga agattatgac ccacaccagg gatgtaggtt
     3001 gtcacttcca tcccattggt caaacgcaca cgcgcaacct tacgaagcgc ggagttaggc
     3061 ttcttcggag ttgtggtgta gacacggaga cagacgccac gcttctgcgg gcaacgcttc
     3121 aacgcgggac ttttcgtctt cgacttaacg agcgtccggc cttttcgcac caactgatta
     3181 atcgtaggca tgcactatac tctttcttga aaatcaggca tttgctcaaa aatgctcctg
     3241 agcgaagccg tcggattata gagatgtgta ctacttatgt caagaacctt cttgttcttc
     3301 acgcattatt ttctgacgca ctgcctacag aagtgatcgg agcctgtcca accgccaccg
     3361 gttgagactg gtcaccggac aactctcctg gagccttagc gctcgcaacg aaggtctctc
     3421 ggtattcctc gaaaccgctg cccgccggaa tcaatcgtcc aacgatgacg ttttccttca
     3481 ggccaaggag attatcctcc ctgccgttta tggccgcctc cgtcaacacc cgggtcgttt
     3541 cctggaaaga tgccgccgag atgaagctgt cggtagtcaa cgcggccttg gtaataccca
     3601 gcaggaccgg ctttccgagg gctggcttgc cgtctttttc caagacacgc tgattttcca
     3661 cgtcgaacat gcccttactt acctgactac caggcaagaa ggacgtgtct cctggatcct
     3721 caatgcgaac ctttcgcaac atctgacgca caatgatttc aatgtgcttg tcattgatgg
     3781 acacgccctg aagccgatac acgtcctgca cttcatcaac aagatatttc tggagctcct
     3841 tcggccccaa cacatccaaa atatcgtgcg gattggcgga tccgtccatg agaggctcac
     3901 cagctcgcac ccagtcgccc tcatgcacgt tgacgtgctt acctttggga atgaagtact
     3961 ccttcacatc ccccattttg ttatcgacca gcaccttccg catacccttc acaaacccgc
     4021 cataggaaac ttcgccgtcg atttcactaa tgacggcctg ctctttgggc ttgcgcgcct
     4081 cgaacaattc ggcgactcgc ggaagacctc cagtgatgtc cttggtcttt gtggtttccc
     4141 gcggaatctt cgccaacaca tcgccgggat gcaccatggc gcccttttca acgaaaatgt
     4201 gcgcgccgac cggcaacaga tatcgagcca ccggtgctcc agctccggag actttggcgg
     4261 tcttaccgct gtcatctttg atggaaacgc gcggccgaag cgtggccccg ctttgctcaa
     4321 tgatgacctt tcgagacaaa ccagtcacct cgtcaaactc ttccttcatg gtgacgcctt
     4381 cgagaatgtc gccatacgcg acctttccgc cggtctccgt gaggatggtc agtgagtacg
     4441 gatcccattc gaccaacttc tggccgactt cgatccgatc accgtccttc aacttgatct
     4501 tggcgccata cacgaccgga tacttttcac gctcacggcc actgtcgtcg acaatcgcga
     4561 ttttcgcatt gcgattcatg acgacccatt caccctcttt attccgaacg gcaatgccgg
     4621 cgttgtgcac atcggcattt ttcttggcat ccaagctcat atacttcagg tgaccggcat
     4681 gcttggcttc caagactgtc tgctcaacaa ccttactagc cgtaccaccg atgtggaacg
     4741 tacgcatcgt caactgagtg ccaggctcgc cgatggattg ggcagcaata acgccgaccg
     4801 gctcgccctt ttccaccagc cgtcctcgcg acaagtcacg gccgtaacag agacggcaca
     4861 cgccacgggc cgactggcat gtcaagaccg atcggatctt tacgcgatcc acaccggctt
     4921 ccaccaccgc cttggcttgc tcttcgtcga tttcctcatt gaacgagaca atgatctcgc
     4981 ccgtcaccgg atctcgaatg tcttcgccgg ccagacgacc caacagacgc tcttcgagcg
     5041 gctgaataat ctcgccacct tcaagcagcg cgcttacgag aattccgtcg gtcgtgccgc
     5101 agtcttcttc ggtcacgatg acatcttgcg cgatgtcgac caatcgacgg gtcagataac
     5161 cggagttcgc ggttttcaac gccgtgtcag ccaaaccttt tcgcgcaccg tgcgtcgaaa
     5221 tgaagtactg caataccgtc aacccttcac ggaagttcgc cgtgatcgga gtttcaatga
     5281 tttcaccgga cggcttcgcc atcagtccgc gcataccgcc aagctgacga atttgctgcg
     5341 agctgcctcg agcaccggaa tccgccatca tgaagatcgg gttgaaggat tcagccttgg
     5401 cgggatcacc gccagcgccc aactccttca tcatctcatt ggccacctgc tcggtcacat
     5461 gcgcccaaat gtcgatgact ttgttgtagc gttcgccgtt ggtaatcaac ccttcggagt
     5521 actgcttttc gatttcattg acttcatgct gagccttgcc aataagatct tccttccggg
     5581 aaggaatgtg catgttgtcg atacagatcg acatgccggc cctggttgca taatggaaac
     5641 ccaggtcctt gatcttatcc aagaaggtga ccgtttcgcg gtggccagcc tgccgataga
     5701 ccgcgtcgat cagctttgac atttccttct ttgtcatcaa tttattcgca tcggcaaacg
     5761 gcatcattgg agggagaatt tccgacagaa tgacgcgccc ggctgtagtc tgcacgagcg
     5821 taccgttgca tcgcaccttg atcctggcat gctcatccaa cgcgccagca tcgtaagcaa
     5881 tgcgtgcctc ttcgggagat ccgaacaact tgccttcgcc ctttgcaccc acacgctcct
     5941 tggtcaacca gtaacaacca agcaccatgt cctgtgacgg aaccgcgatc ggtttcccgt
     6001 tcgctggcga cagaatatta ttgatcgaca tcatgagcac gcgggcctca acctgcgctt
     6061 caaccgacaa cggcacgtgg accgccatct ggtctccgtc gaagtcggcg ttaaacgccg
     6121 cgcagacgag tggatgcagc cggatggcct taccttccac caagaccgga tcaaaggcct
     6181 gaattccgag tctgtgcaac gtcggagcgc ggttgagcaa caccggatgc tcacggatga
     6241 cttcatccag cacatcccac acttcaggac gctctttctc taccagacgc tttgcactct
     6301 taatggtcgt agctgcgcca cgctcttcca gcttgtggaa aataaacggc ttgaacagct
     6361 ccaatgccat ctttttgggc agaccgcatt gatgcaaccg cagctccgga ccgacgacaa
     6421 taaccgttcg gccggaatag tcgacgcgct ttccgagcaa attctgccgg aaacgtccct
     6481 gcttgccctt cagcatgtca ctcaatgact tcaatgggcg cttgtttggc ccacggatgg
     6541 cacggccgcg acggccgtta tcgaacagag cgtccaccgc ctcctgtagc atgcgcattt
     6601 cattgcgaat aattacgccg ggagccttga gttcgatcaa gcgcttcaag cggttgttcc
     6661 gattgatgac gcgccggtac aggtcattga ggtctgacgt cgcaaaccgg ccaccatcca
     6721 acggaaccag cgggcgtaat tccggcggca ataccgggat gacatccatg atcatccatt
     6781 caggcttgtt gccggaacgg cgaaatgcct ccaagacctt caagcgcttg gcgtatttct
     6841 ttttgagcgc agcggatgcg gaggccttcg ccttcacatg caattcgtcc cactgggtat
     6901 tgatgtccac cttgcgaagc aattcccgaa tggcttcggc gccaatccca accttgaacg
     6961 caccgctgcc atattcggac tgcaatgaac gcaactgctc ttctgagacc agctcttgct
     7021 cactcatgct tgtcgatcca ggatcgacca tcacgtagct ctcgaagtag aggatctttt
     7081 cgagctgctt gaggctcata tcaaggagcg tcccgatgcg gctcggcaca cctttcaaaa
     7141 accagatatg tgcgaccggc gcagccaact caatgtggcc catccgttcg cggcgcacct
     7201 ttgactgaat gacttcgaca ccgcatttat cgcagacgat gccgcgatgc ttcatgcgtt
     7261 tgtatttacc gcagttgcat tcccaatctt tgatcgggcc gaaaatcttg gcgcagaaca
     7321 agccgtcctt ttcaggcttg aacgaccgat agttgatcgt ttccggcttc ttgacttcgc
     7381 catatgacca cgagcggatt ttttcgggcg acgcaatgcg aatgcgcatc gagtcgaacg
     7441 acaccgaatc ccgcggcttc tcgaataatg tgtatacacc ttccaaggtt gatccctcct
     7501 taacggtcgc ctgagcgcag ttctgagtgg tgagctatcc gat
//
LOCUS       DAOQEW010000186         7493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3505_length_7493_cov_9.988303, whole genome shotgun sequence.
ACCESSION   DAOQEW010000186 DAOQEW010000000
VERSION     DAOQEW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_7493_cov_9.988303"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLS35_15945"
     CDS             complement(<1..698)
                     /locus_tag="PLS35_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNI20214.1"
                     /translation="MPTISLQRDDLEALIAGHGETASRISIDQLEQWLMLVKGELKGH
                     NIETGELRIELQDSNRPDLWCCEGIARQIRTKQQGAAISYPFFKKQKRAAGKLVVAPG
                     LDQVRPYVAACTAIGYRVTASGLAQLIQTQEKLADIFGRKRRTVSIGLYRLAPIVFPV
                     SYDLVKPDEVRFTPLGMETVMTLREILMVHPKGVEYGGIVSGHDRLPILRDAKGQVLS
                     FPPIINSREIGEVQV"
     gene            complement(711..2255)
                     /locus_tag="PLS35_15950"
     CDS             complement(711..2255)
                     /locus_tag="PLS35_15950"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNI20215.1"
                     /translation="MDNLHPLESKVLLALAPQPGSPSTLDQLADSTGLEPSQLSMAVE
                     WLLAKSLIAVHAETVAYIASLTPIGEVFFEKYAPIERVLSAAREAGQTGKRLTIQDIQ
                     AKEALEPSDVSKAVGTLKKEGAILIVQGGCIESTGRNSSTAEAMRALLQELRSGQREL
                     QTFAEPLRAVLQQHAVKRGNAREPFRVDERVTRSFLLTPSGQEVADQLSRDGVAEEVS
                     QLTPELLKEGAWRTKQFRKYTISLRAPRIAAGKRHPYREFLDLVKLKLVSMGFQEMRG
                     TLVETEFWNMDALFMPQFHPARDIHDVYFVKNPTHARNIDEPYLTNVTQVHENGAGAG
                     STGWGYQFDAERAKRLVLRSQGTAVSARRLAANPSVPGKYFSIARCFRYDQVDATHAT
                     DFFQVEGIVLGTDINFRTLLGLLNLFAREVAQAKEVKFLPAYFPFTEPSVEMHVRHPK
                     LGWMELGGAGLFRPEVTTPLGVSVPVIAWGLGLDRMAMVALGIHDIRDLFSADLEFIR
                     TMRGSF"
     gene            complement(2283..2987)
                     /gene="rpe"
                     /locus_tag="PLS35_15955"
     CDS             complement(2283..2987)
                     /gene="rpe"
                     /locus_tag="PLS35_15955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNI20216.1"
                     /translation="MAGRTVCIAPSILSADFARLAEEVAKVEEAGADWLHVDVMDGHF
                     VPNLTIGPPVVEALRKVTSLPLDVHLMMTNPDAFIGEFAEAGADYLTVHVETCPHLHR
                     TVQLIKERGVKAGVTLNPATPAITLSEIVGDADLILIMSVNPGFGGQKFIPSSLQKVA
                     AVRAMIDRTHSHALLEVDGGVKPDNADSILAAGADVLVAGSAVFSDHDYAAAITALRA
                     GRVSPPRSSRTMAGQR"
     gene            complement(2987..4426)
                     /gene="rsmB"
                     /locus_tag="PLS35_15960"
     CDS             complement(2987..4426)
                     /gene="rsmB"
                     /locus_tag="PLS35_15960"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNI20217.1"
                     /translation="MGTDPQPASPGHAVSPGRHAAVKALLAIEEAGTLSDDLFDQISA
                     KAGLDLRDRAFMVELVRGVLRYRATLDWRLGLLSDRRIAKLPTLVQIMLRLGAYQVLY
                     LDRVPASAAVNESVQMMKQLRRRLGRDWSGFVNAVLRAMLRASPPDWPDLARNPVEAL
                     AVQYSCPTWLVERWCRQWGAERAATLCRASVEVPPLTLRVNRLRTTRAALLADLGAAH
                     VEAEPTSISEVGIQLGRTSLVADLPGYPAGHFYVEDEAGQLVPLLLDVQPEHRVLDAC
                     AAPGGKATHIAALMANRGEIIAVDRVASRLDRVMDNCRRLGVTIVTPVVADMRELKAE
                     EAGAREPAISPALPEVQSRPFDRILLDAPCSGLGVLRRHPEAKWYKTPDSIAQHRQVQ
                     LDLLATTTRLLRPGGVLVYSTCSIEPEETESVIDEFCQSHREFQRDSIAPWLPPAGLP
                     FLTPRGDLSTMANRNNMDAFFAARLRRVG"
     gene            complement(4485..5435)
                     /gene="fmt"
                     /locus_tag="PLS35_15965"
     CDS             complement(4485..5435)
                     /gene="fmt"
                     /locus_tag="PLS35_15965"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNI20218.1"
                     /translation="MRIVFMGTPDFAVPSLEALLKSDDQVVGVVTQPDRPKGRGQEVI
                     PSPVKVVCQREGIPVLQPLKMKDPAFLDALREWQPDVIAVTAFGRILPPVILTLPPRG
                     CINVHGSLLPKYRGAGPLQWAIIRGERETGITTMFMAEGMDTGDMLLREAVEIRPDDT
                     AGTLAPRLADVGGRLLVETLRRLKAGTLTPERQDDSQATMAPLLKKEDGLIDWTLSAQ
                     EIANRVRGLSPWPGAYSYVNGERWTLCRVAVGDASQQAAPGTVTKVTKDRIDVATGAG
                     AIQILDIQPSNSRRMTVAQYLAGHRVAEGISLHAVPPPQA"
     gene            5523..5599
                     /locus_tag="PLS35_15970"
     tRNA            5523..5599
                     /locus_tag="PLS35_15970"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5557..5559,aa:Met,seq:cat)
     gene            complement(5618..6718)
                     /locus_tag="PLS35_15975"
     CDS             complement(5618..6718)
                     /locus_tag="PLS35_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNI20219.1"
                     /translation="MMALFRRNRVWWMGFSYCGKQVRRSTEVTDKKVAEKIYHKVRTQ
                     IAEGKWYEPARGDDKTVKELLERYLRDHSAVNKSSSSQRSDRSVSVHLIRAFGDLTLK
                     ELRPSLIAAHKAKRRAEGAAAKTINIELGILSHAFQLAVKEWEWVTENPVIRVSREKV
                     RNVIERWLTAEEEQRLLAASPQWLQEIIVFAANTGLRQGEILDLQWSRVDLFRRTITL
                     LEQKNGAKDTLPVNAKTFEVLKARARVRSLKSDYVFFNGAGNRMDARDLLRVFYPVMR
                     KADVKNFRFHDLRHTFATRLVQAGVDIYTVQKLGRWKTISMVMRYAHHHPESLRAGVE
                     ILDRVPAGVSTNLAQSASQTGADRGVETAVSG"
     gene            complement(6715..7011)
                     /locus_tag="PLS35_15980"
     CDS             complement(6715..7011)
                     /locus_tag="PLS35_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI20220.1"
                     /translation="MLTVKELSAWLNIKPSTLYLWVSQNKIPCRRIHGLVRFEPEAIQ
                     AWLNGFSSTPSNQPLRVPRQRAEDVDHLIAVAKAVHYTSRREKPDKDRATQGGQ"
     gene            complement(7054..>7493)
                     /locus_tag="PLS35_15985"
     CDS             complement(7054..>7493)
                     /locus_tag="PLS35_15985"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HNI20221.1"
                     /translation="LELKKDRAEQCARALATLDEADWKELVIGLLRSYVDFRQIPKDA
                     EDEERYRAPVLEWYALLTEGFQKGRLAQEQQVQTLQNVKRWVSDTLTPMLAVICATPG
                     GEEWLLNEIVRGIARWNDRHRNLLKQPPNRFHRSAGGHAGSPC"
BASE COUNT         1594 a         2326 c         2018 g         1555 t
ORIGIN      
        1 acctgcacct cgccgatttc acggctattg atgatcggcg ggaacgacag gacttgccct
       61 ttggcatcac gcaatatcgg gagtcgatcg tgaccgctga cgatgccgcc atactccaca
      121 cctttcggat ggaccatcag gatctcgcgc agagtcatca ccgtttccat gcccaacggg
      181 gtgaaccgca cttcatccgg cttcaccaga tcgtacgata cgggaaagac aatcggcgcg
      241 aggcgataga ggccgatgga caccgtgcgg cgcttgcgcc cgaaaatatc cgccagcttt
      301 tcctgcgtct ggatcaattg cgccagaccg gaggccgtca cacgatagcc aatcgccgta
      361 caggccgcca catacggccg cacctggtcc agcccgggtg ctacgacgag ttttccagcc
      421 gcacgttttt gcttcttgaa aaagggatag gaaatggccg cgccctgctg cttggttctg
      481 atctgccggg caatcccttc gcaacaccac aaatccggcc ggttgctgtc ctgcagctcg
      541 attcgtaatt caccggtttc gatattgtgc cccttcaact cgcctttcac caacatcagc
      601 cattgctcaa gctggtcgat cgaaatacgg gacgccgttt cgccatgacc ggcgatcaac
      661 gcctcaagat cgtctcgttg tagagaaatg gtgggcatga tgtgatcgtc ctaaaaactt
      721 ccgcgcatgg tccgaatgaa ttccaaatcc gccgaaaaga ggtcgcgaat atcgtgaata
      781 cccaacgcca ccatcgccat acgatccagc ccgagtcccc aggcaatgac cggcacagag
      841 actccgagcg gcgtcgtgac ttcaggccgg aacaatcccg cgcctcccag ctccatccag
      901 cccaacttcg gatggcgcac atgcatttca accgacggtt cggtgaacgg gaaataggcg
      961 ggaagaaact tcacttcctt ggcctgcgcc acttcacggg cgaacaggtt taacaatccc
     1021 aacaacgttc gaaagttgat atccgttccc aacacaatcc cttctacctg gaaaaaatca
     1081 gtagcatggg tggcgtccac ctggtcgtaa cgaaaacagc gggcgatcga aaagtacttt
     1141 ccggggacgg atgggttggc agcgagcctg cgcgcagaaa ccgccgtccc ttggcttcgc
     1201 aacaccaacc gcttcgctcg ctccgcatcg aactgatagc cccaaccggt cgatccggcc
     1261 cctgcgccat tctcatggac ctgtgtcaca ttcgtcagat acggttcatc gatgttgcgg
     1321 gcatgggtcg ggtttttcac aaagtacacg tcgtggatat cgcgcgcggg atgaaactgc
     1381 ggcatgaaca gagcgtccat attccaaaac tcagtctcca cgagcgttcc acgcatctcc
     1441 tggaagccca tgctgaccag cttgagtttc acgagatcca ggaattcccg atacgggtgc
     1501 cgtttcccgg ccgcgatcct cggagcccgc aagctgatcg tatacttccg gaactgcttc
     1561 gtccgccagg caccttcctt gagaagctcg ggcgtcaatt gggaaacttc ttctgcgacg
     1621 ccgtcacgcg acaactggtc agccacttcc tgaccggaag gcgtcagcag aaacgatcgc
     1681 gtcacccgtt cgtcgacccg gaagggctct cgcgcgttcc cacgtttgac cgcatgttgc
     1741 tgaagcacag cccggagcgg ttccgcaaag gtctgcaact cacgttgtcc gcttcgcaat
     1801 tcctgcaaga gggcacgcat ggcctctgcc gtggaactat tccggccggt gctttcgatg
     1861 cagccaccct gtacgatgag aatggcgcct tcttttttga gggtgccgac cgctttactg
     1921 acatccgacg gttcaagcgc ttccttggcc tgaatgtcct ggatggtcag acgtttgccg
     1981 gtctgcccgg cttcccgtgc cgccgacagc acgcgttcaa tcggcgcgta tttctcgaaa
     2041 aacacctcac cgatcggcgt caacgacgca atatacgcaa ccgtttcggc gtgaacggca
     2101 atgagggatt tggccaagag ccattcgacg gccatactta actgcgaagg ttccagcccg
     2161 gtggagtccg cgagttgatc gagcgtggag ggcgagccag gctgcggagc caatgccagc
     2221 agcactttgc tttcgagggg atgaagatta tccatgaatg acggtgcggt ccgatgcctc
     2281 tgctatcgct ggccggccat agttctgctg ctgcgtggcg gtgatacccg accggccctg
     2341 agggctgtaa tcgcggcggc ataatcgtga tcggaaaata cggcggatcc tgctaccaac
     2401 acatcggccc ccgccgcaag aatggaatcc gcgttatccg gtttcacccc accgtccacc
     2461 tcaagaagcg catggctgtg ggtccggtcg atcatggccc ggactgcggc gaccttttgc
     2521 aaggacgagg gaataaattt ttgcccgccg aatccgggat tcacggacat aatgagaatg
     2581 agatcggcgt cgcccacaat ttcggaaagg gtgatcgccg gtgtggcggg attcaacgtg
     2641 accccggcct ttacgcctcg ttctttgatc aattgcacag tccgatgcag gtgaggacag
     2701 gtttccacgt gcacggtcag atagtccgcg ccggcttcgg caaactcccc gatgaacgca
     2761 tccggattgg tcatcatcag atgcacgtcc agcggcaggc tggtcacttt tctcaacgct
     2821 tcaacgaccg gggggccgat cgtcaggttc ggcacgaagt gaccatccat cacatccaca
     2881 tgcaaccaat cggcgcccgc ttcttcgact ttggcgacct cttccgccag acgtgcgaaa
     2941 tctgccgata gaatagatgg cgcaatacag acggtcctgc cagccattac ccaaccctcc
     3001 gcagacgggc ggcaaaaaac gcatccatgt tatttctatt ggccatcgta gacagatccc
     3061 ctcgaggggt cagaaacggc agaccggctg ggggcaacca gggcgcgatc gaatcgcgct
     3121 gaaattcacg atgagactga caaaactcgt cgatgaccga ctcggtttct tccggctcaa
     3181 tcgagcacgt actatagacc aacactccac caggccgcaa gagacgagtc gtcgtggcga
     3241 gcaggtccag ctgcacctgt cgatgttgcg cgatggaatc cggcgtcttg taccatttgg
     3301 cttctgggtg acgccgcagc acgccgaggc cgctgcacgg ggcatcgagt aaaatccgat
     3361 cgaacgggcg cgattgcacc tccggcagcg cgggagagat cgctggctct ctcgcccccg
     3421 cctcttcggc tttcagctct cgcatgtcag ctacgactgg cgtgacgatc gtcacaccca
     3481 ggcgccgaca attgtccatc acccggtcca gacgtgatgc gactctatca accgcgatga
     3541 tttcacctcg attggccatg agcgcggcga tatgcgtcgc tttgccgcca ggtgcggcgc
     3601 aggcatccaa cacacggtgc tccggctgga cgtccaagag cagaggcacc agttgcccgg
     3661 cttcatcttc gacataaaaa tgtcccgccg ggtatccagg gagatcggcg acgagactgg
     3721 tgcggcctag ttgaatgccg acttcgctga tcgacgtggg ctccgcttcg acgtgcgccg
     3781 cgcccaagtc agccagtagc gccgctcgcg tggttcgaag ccggttcaca cgaagtgtga
     3841 gcggaggcac ttcaaccgag gcccggcata aggttgcggc tcgttcagcc ccccactgtc
     3901 gacaccagcg ttccacgagc catgtgggac atgagtactg aacggccagt gcctcgacag
     3961 gattcctcgc gagatccggc caatccggag gcgacgcccg cagcatggcc cgcagtacgg
     4021 cattgacgaa tccactccaa tcgcgcccca agcgccgtcg cagctgcttc atcatctgca
     4081 cggattcgtt cactgctgct gaggccggca cccgatccag gtacagcact tgatacgcgc
     4141 ccaagcgcaa catgatttgc acgagagtgg gaagtttggc gatacgccga tcggacaaga
     4201 ggcctagacg ccaatccagc gtcgcgcgat accgcaacac accgcgcacg agttccacca
     4261 tgaaggctcg atcgcggaga tcgagcccgg ccttcgcaga aatctgatcg aacaagtcat
     4321 cggacaacgt gccggcttcc tcgatggcca ataacgcctt gacggcggca tgccgcccgg
     4381 gagaaacggc gtgaccgggt gacgctggtt gcggatcagt tcccatgggc actagacatg
     4441 aacatcgccg gaaatggtga atggaatgcg cgtgccgcgc tgggtcaggc ctgtggaggc
     4501 ggaacagcgt gcagactgat accttcagcc acacgatggc ctgccagata ttgcgccacc
     4561 gtcatacggc ggctattgga tggttggatg tcgagaatct gaatggctcc cgcaccggtc
     4621 gcgacatcga tgcggtcttt cgtcaccttc gtgacagtgc ccggagccgc ttgctgagat
     4681 gcatcgccga ccgcaacgcg gcagagagtc caccgttcac cgttcacata gctatatgcg
     4741 ccgggccagg gagaaagtcc ccgcacgcgg ttggcgatct cctgggccga tagcgtccaa
     4801 tcgatgaggc cgtcctcctt cttgagcaac ggcgccatag tcgcctgaga atcatcctga
     4861 cgttccggcg tcaacgtccc cgccttcagg cgacgcaggg tctcgaccag caaccgtccg
     4921 cccacatccg caagccgagg ggcgagtgtg ccggctgtat cgtctggacg gatttctaca
     4981 gcctcacgca gcagcatatc gccggtgtcc atcccttcag ccatgaacat agtcgtaatg
     5041 ccggtttccc gctcaccgcg gataatggcc cattgcagag gaccagcccc gcgatacttc
     5101 ggcaaaagcg acccatggac gttgatgcag cctcgaggcg gcagcgtcag aatgaccggg
     5161 gggaggatac ggccgaaggc agtcaccgca atgacatccg gctgccactc gcgtaatgcg
     5221 tccagaaaag ccggatcctt catcttcagt ggttggagca cgggaatgcc ttcgcgctgg
     5281 cagacgactt tcaccgggga aggaatgacc tcctgcccgc ggcctttcgg gcgatccggc
     5341 tgtgtcacca cgccgacgac ctgatcatcg gacttcagta acgcctccaa tgagggcacg
     5401 gcgaagtcgg gtgttcccat gaatacgata cgcatgctct ggctttatca ttctcccttc
     5461 gcgaatgcaa ttctcgtcgc ggcttccttg actctcgttt cacacgcctg ttagtatccg
     5521 gtcgcggggt ggagcagcct ggtagctcgt tgggctcata acccaaaggt cgtcggttca
     5581 aatccggccc ccgcaaccaa cctttcccac acttctttca accacttaca gcagtttcaa
     5641 caccgcgatc cgcacccgtc tgagaagccg attgtgctaa atttgtgcta actcctgccg
     5701 gaacgcgatc caagatctct acaccagcac gcaaactctc aggatgatga tgggcatacc
     5761 gcatcaccat ggaaatggtt ttccatcgtc cgagtttctg cacggtgtaa atatcgactc
     5821 cggcctgcac aagacgggtg gcaaaggtat ggcgcagatc gtggaaacga aagtttttca
     5881 cgtctgcctt gcgcatgaca ggatagaaca cgcgaagcag atctcgcgca tccatccgat
     5941 tgcccgcacc gttgaagaac acgtaatcgc ttttgagcga acgaaccctg gctctagctt
     6001 tcagtacctc aaacgtcttt gcattaacag gaagggtatc cttcgctccg ttcttctgct
     6061 ccagcaaagt aatggtccgc cggaagagat caaccctgga ccattgcagg tcgaggattt
     6121 ccccctgccg aagccccgta tttgcggcaa agacaatgat ctcctgaagc cattgcggtg
     6181 aggccgccaa caatcgctgc tcctcctccg ctgtaagcca ccgctcaatc acgttacgca
     6241 ctttctccct cgatacgcgg atcaccggat tttccgtcac ccactcccat tctttgacgg
     6301 caagctgaaa ggcgtggctc aagatcccta actcgatatt aatggtcttg gccgccgctc
     6361 cttctgctcg ccgcttcgcc ttatgtgctg caataagaga agggcgcagt tccttgaggg
     6421 tcagatcccc gaatgctcga atgagatgca ccgacacaga cctatcactt cgctgagagg
     6481 aagaagactt attgaccgct gaatgatctc ggagatagcg ctccaacaat tccttcaccg
     6541 tcttatcatc tccccttgct ggctcgtacc acttcccttc agcgatctga gtcctcacct
     6601 tgtgataaat cttttcagcc actttcttat ccgtcacttc agtggatctt cggacctgtt
     6661 tgccacagta agaaaacccc atccaccaga ctcggtttct ccgaaacagt gccatcattg
     6721 ccctccttgt gtggccctgt ctttgtctgg tttctcgcgt ctggaagtat agtgaacggc
     6781 cttcgcgaca gcaatcaggt ggtctacgtc ctcagctctc tgccgaggca cacgaagtgg
     6841 ctggttcgaa ggcgtggaag aaaagccgtt cagccaggcc tgaatcgcct caggttcaaa
     6901 gcggacgagg ccatgaatgc gacggcacgg aatcttattc tgtgaaaccc agaggtagag
     6961 tgtggacggc ttgatattga gccaagccga gagctctttg acggtaagca tggtacagat
     7021 caccggggga gtctcccgac acccccagtc cccctaacat gggctgcccg cgtgaccgcc
     7081 ggcagaccgg tgaaaccgat tgggtggttg cttgagcaaa ttgcggtgtc ggtcgttcca
     7141 ccgagcaatg cccctgacaa tttcgttcag tagccattct tcccctccag gggtggcaca
     7201 aatgaccgcc aacatcggcg tcagggtgtc actcacccat cgttttacgt tttgcagggt
     7261 ctgcacctgc tgctcctggg ccaatcgccc cttctgaaat ccctccgtca ggagggcgta
     7321 ccactccaac actggagccc ggtagcgttc ttcatcctcg gcgtccttcg ggatctgccg
     7381 gaagtctaca taggaccgaa gcaagccaat caccaactcc ttccaatcgg cttcgtctaa
     7441 agttgctaac gctcttgcac attgctcggc gcgatccttc ttgagttcca att
//
LOCUS       DAOQEW010000187         7468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3522_length_7468_cov_8.682585, whole genome shotgun sequence.
ACCESSION   DAOQEW010000187 DAOQEW010000000
VERSION     DAOQEW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3522_length_7468_cov_8.682585"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1137)
                     /locus_tag="PLS35_15990"
     CDS             complement(181..1137)
                     /locus_tag="PLS35_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20222.1"
                     /translation="MSVEPDLRSCQLKPGEYAVINFITVDAATNPIPLKSLIEHLRKC
                     GSALRQINPRCPSTLVAEFPAGITVRAVIPAAANGGLFGPQDPQSSPAEPASAAIDLR
                     SNNASTTPTAQTLEATALRNAEYLATEARTISIKAVDVGNDLASRAEIHAMDAEDLPE
                     LLANSPDQAISRATYTLARGAEMKLDYITATRTIGGNRQTPANHHSRETFALRGCRLV
                     STNTAFTFTMRGHRDDPEWHRLIQMFPDCMEVRLEEESPELPFVTESVLREIPIDLDI
                     CVAERVGTGKHTAIPLRVCNRDAVIDSLASRLQLLTELAANE"
     gene            complement(1134..5957)
                     /locus_tag="PLS35_15995"
     CDS             complement(1134..5957)
                     /locus_tag="PLS35_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNI20223.1"
                     /translation="MDDTNPCTLMPSPEGEDYLRPAEPQSTASHPEAETQSDELRHAR
                     QLLIRTFAVPISGIGLAALAHELRADLDALDRGIVSTELPDALRKLLDTLCQDRADAV
                     RDLAYLRSLPACPLRDLLTQPSLTLPPPERRCLRSVRALLALHLLSESNGLGRTLAAE
                     LSRWMRGKGLRFAQPLELSADELRLRRDSLPPGTPQAALMAVLVEALSRKFSNPFVTR
                     RAVQVISHSSGEERREAHEAASEVGSDQQARSVADPLRGRKATIGKAGCRVLSGTPAL
                     YEYAQPFELEAIVPKILDRWRTGQEDEALGALLTLHLGVMPSSFCQVPLTSESGAGIR
                     INLAAGCLEWNLDEVIGRHTPDNSFTRSSSDRYVQVPLPSETVAELRRRQQSTGGKTL
                     ADLFDSEKGHLGKKTKLMLRELSLTSHRPALKRLSFSWGRYVLSRTGDETYAAAMGVD
                     FTLGTTANLNYCLLRAARIRSIASECYRRVGLSGNCGGQPLPDVGSLRLIPDATARAF
                     IRDSLSIVRTAMTGVPRRCAIEVVHEAHNLVATHFYAALKFILAGRSLQEETLTRRCI
                     DVETGLFVVTDKRTARYHEHRLGCLPPTLREWLRAYLAWLSSVAYRMASIDPHLARAI
                     RSVAEGDTKVEAHPLFFCFSANGTTAPLGAHDISPILGRHQMASNAGRHFLDVLFRDA
                     GLDSAAVMGWMGRAMPGQEIIGAASSVAPLEFLLRCATVIEDWLSELALPPAPAMSSR
                     KLHDARHHTHPEYRPVLLDDCPASLKDAKGAEPCPIGRDTIHLAAQFPDLLRFWRTDS
                     PPGGWAGIALSLIFEDAIVLDEEVIAVLQELRQGILYRHREIHFVDARPAPLGIRRVW
                     LSPITVRLIYQTPALGGSLVTLDTISEAFRLFLTMSGADSAGGSPVSYVTACARAYFA
                     LRTPGILRAWMDGSKFSRTVRPHCVARQYLGLCEPPAPRVSRNSRTVRRPDAFRKHVR
                     DICDDVPASKSHRRAIDELREWLDETGSADDPSSEHELSVGYCRSLLDRHDNVHTILR
                     YLTAAAPFLHALSRVIASGGLEGVDWLELINEAPEDKDAHDGHDSAPTRAAINQALDW
                     LGIDYRLGRRVGPPPAALRYTDLLTDRETRLAISMLAARRRTPGDEWHRAEAALRLMS
                     DVALRWDEIINLRLTDVWIGSSPAPHIVVTNESRAALKTRNARRVLPLNGMETIREVR
                     ALYDLRSSRFGGDKMIPLLGADLEDRATDSGDRIHDLVTEALRRASGAWGIHPHCTRG
                     TVITSEIDAVLSPSRHERRPIDLRQALYSISARAGHGHPDVTVGEYFAGMDHQRRDWA
                     TEILRAADCPPRPAFLEALTGIAAATYRKRLSRGLNDDWFDVLEGLQSDAIPTVGAKV
                     CELGSLVQAGLEEIPWTDADLQEERLTATAIYLGLRMLKEEIHSARLTARLTRQETES
                     AEQMLSELQRARLTRLAGRRDLSRQRFITEMAKARLPAAIAACNLDADLLREVVLAAH
                     TAGDPLTFARPEHALRMSPLLGPIRSADIQTVCLVRSSGQSAVDTCWLDRFRNAGFGS
                     VQCRPPRDFPRGSQCKVLFCPKSSDSAGHRRASPRHAFLVTISILAVHFRKELKQ"
     gene            complement(6721..6939)
                     /locus_tag="PLS35_16000"
     CDS             complement(6721..6939)
                     /locus_tag="PLS35_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI20224.1"
                     /translation="MVRCHLSRLMGEHKLKIADVARETGLHRNTITLLYNETANRVDL
                     ETIDRLCALFECTVGELFEYIPDATNTA"
     gene            7088..7321
                     /locus_tag="PLS35_16005"
     CDS             7088..7321
                     /locus_tag="PLS35_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20225.1"
                     /translation="MSLTYLTTDELAGRIKYDPRTIRERLKDSVLLEGTHYIRPFGGR
                     KILYIWEHIERDMGCTSEQSFAIPMANGGVCHG"
BASE COUNT         1430 a         2106 c         2420 g         1512 t
ORIGIN      
        1 tcgatgctcg tcacgagcgg ttcagcacac ccctaccgcg cgagcggttt agtcctcttg
       61 ccattatcgc tccgtgacca acgccgcggg tagtcctatc ggagcagctg tgagctatcc
      121 atcgctatcg ggtcgaggcg ttcccgcaat ctcgttttag cacttggctg gtcgcagccc
      181 ttactcgttt gcggccaact cggtgagtag ctggaggcgc gatgccagag aatcgatcac
      241 cgcgtcacgg ttgcatacgc gcagcgggat ggcagtgtgc ttccctgttc ccactcgctc
      301 cgctacgcag atatcgaggt cgatcggaat ctctcgcagg accgattccg tgacgaacgg
      361 cagttccggg ctttcctcct ccagacgcac ctccatgcag tccggaaaca tctggatcag
      421 ccgatgccat tcggggtcgt ctcgatggcc acgcatcgtg aaggtgaagg cggtgttggt
      481 tgagacaagc cggcagccgc gtagcgcgaa tgtttctcgg gagtgatggt tagcaggcgt
      541 ctggcggttg ccgccaatgg tgcgagttgc ggtgatgtag tccagcttca tctcggcacc
      601 acgcgcgagg gtgtacgtgg ccctcgatat tgcctgatct gggctgttgg ccagcagttc
      661 cggcagatcc tcggcatcca tcgcgtgtat ttctgcccgt gaggccaagt cgtttcctac
      721 gtcaacggcc ttgatcgaaa tggtgcgcgc ctcggtggcc aaatactccg cattccgaag
      781 ggctgtggct tcaagcgttt gagcggtggg tgttgtactt gcgttgttgg agcgaagatc
      841 gatcgctgct gaggcgggtt cggcggggct ggattgcggg tcttgtggcc cgaaaagccc
      901 gccgttcgca gctgcgggaa tgactgcccg gacggtgatg ccggccggaa attccgccac
      961 cagggttgaa ggacaacggg gattgatttg cctgagtgca gatccgcact ttcggagatg
     1021 ttcaatcagc gactttagag gaatcggatt ggtggcggca tccactgtga tgaagttgat
     1081 gacggcatac tctccaggct tcagttgaca gctacgcaga tcaggttcga cgctcattgt
     1141 ttcagctcct tgcggaaatg gactgcgaga attgagatgg tcacgaggaa tgcatgccgc
     1201 ggcgatgcgc ggcgatgacc cgccgagtcg gatgacttgg ggcagaacag caccttgcat
     1261 tggctgccgc gcgggaagtc ccggggcgga cgacattgga ccgacccgaa ccccgcgttg
     1321 cggaagcgat ccaaccagca tgtgtcgacc gctgattgac ccgaagatcg cacgaggcac
     1381 actgtttgaa tgtccgcaga cctgatcggg ccgagaaggg ggctcatccg gagggcatgt
     1441 tcaggccgtg cgaacgtgag aggatccccc gcggtatgtg ctgcgaggac cacctcgcgc
     1501 aggaggtcgg catccagatt gcaggcggca atcgctgcag gcaatctggc tttcgccatt
     1561 tcggttatga agcgctgtcg ggagaggtcg cgtcggcctg caagtcgggt gaggcgtgct
     1621 cgctgcagtt cagaaagcat ctgttccgcg gattctgtct cctgacgggt cagtcgcgcg
     1681 gtcaatcgag ccgaatggat ctcctccttg agcatgcgca gaccgagata gatggcggtc
     1741 gccgtgaggc gctcctcctg gagatccgcg tcggtccagg ggatttcttc gagcccggcc
     1801 tgcacaagcg atccgagttc gcacaccttg gcgccaacgg tgggtatggc gtccgactgc
     1861 aggccctcaa ggacgtcaaa ccagtcgtca ttgaggcctc tgctcagtcg tttgcgatag
     1921 gttgccgcgg cgattccggt tagcgcttcc agaaatgcgg ggcggggtgg gcaatcggcg
     1981 gcccgcagaa tttccgtggc ccaatcccgt cgttggtggt ccatccctgc gaaatactcc
     2041 ccgacagtga cgtcagggtg tccgtgtcct gctcgtgcgg agatggaata gagggcctga
     2101 cgcaagtcga tcggccgacg ttcgtgcctg gacggagaca gaactgcgtc aatttccgaa
     2161 gtgattaccg tgccgcgagt gcaatgagga tgaatgcccc acgccccgct cgctcgccgc
     2221 agcgcctcgg taaccaggtc atggatgcgg tcgccggagt cggttgcgcg atcctcgaga
     2281 tcggcgccca gaagggggat cattttgtct cctccgaagc gcgagcttcg caggtcgtag
     2341 agcgcccgta cttcgcgaat cgtctccatg ccatttaacg gcagcacgcg ccgcgcattt
     2401 ctcgtcttga gggctgcgcg cgattcgttg gtcacgacga tgtgcggggc gggggagctc
     2461 ccaatccaga catcggtcaa tcggagattg atgatctcgt cccatcgcag tgcgacgtcg
     2521 gacatcagcc gcaaggccgc ttcggcccga tgccactcgt caccgggggt ccttctgcgt
     2581 gccgccagca tcgaaatggc caggcgagtt tcacggtcgg tcaggaggtc cgtatacctg
     2641 agtgctgctg gcggtggtcc gactcttcga ccgagacgat agtcgatccc cagccagtca
     2701 agcgcctggt tgatggccgc tctggttggt gcgctgtcgt ggccatcatg cgcgtcctta
     2761 tcctcgggcg cctcgttgat cagttcaagc cagtcgactc cctcaagtcc gccggaggca
     2821 atgacgcggc tgagcgcatg aaggaagggg gcggcggccg tcaggtagcg caggatcgtg
     2881 tgtacattgt cgtgacggtc cagcagagac cggcagtatc cgaccgacaa ctcgtgttct
     2941 gagctcggat cgtctgccga tccggtttca tcgagccatt ccctgagttc atcaatggcc
     3001 cttcggtggg acttcgacgc cgggacgtcg tcgcaaatgt cccttacatg ttttctgaac
     3061 gcgtcaggtc tgcggactgt tcgcgaattg cgcgagaccc ggggagcagg cggctcacac
     3121 aaccccagat actgtcgcgc aacgcagtgt ggcctgacag tgcgagagaa cttggaaccg
     3181 tccatccacg cgcggaggat gcccggggtg cggagtgcaa agtacgcgcg ggcacatgcc
     3241 gtgacataac tgactgggga gcctccggct gaatcggctc ccgacatggt cagaaacagt
     3301 cggaacgcct cggaaattgt gtcgagcgtg acgagcgaac cgccgagtgc aggtgtttgg
     3361 taaatcaggc gcaccgtgat tggcgacagc cacacgcgtc gaattccgag tggagcgggt
     3421 cgggcgtcga cgaaatgaat ctcgcgatgg cggtacagga tgccctgccg gagttcctgc
     3481 aggactgcga tgacctcctc atccagcaca atcgcgtcct cgaagatcag gctcagggcg
     3541 attcccgccc agccgcctgg aggactgtcg gtccgccaga agcgaagcag gtcggggaat
     3601 tgggccgcca ggtgaatcgt gtcccggccg atggggcagg gttcggctcc ctttgcgtcc
     3661 ttgagcgacg cggggcagtc gtcgagaagc acggggcgat actccggatg agtgtggtgg
     3721 cgggcgtcat ggagtttgcg ggacgacatg gccggtgccg gcgggagcgc cagttcgctt
     3781 aaccagtctt cgatgacggt cgcgcaccgg agcaggaatt cgagcggcgc aacgctgctg
     3841 gctgcgccaa tgatttcctg tccgggcatc gcccggccca tccagcccat taccgccgcg
     3901 ctgtcgagcc ccgcgtcgcg aaacagcaca tcgaggaaat ggcgtcctgc attcgacgcc
     3961 atctggtggc ggccgaggat cggggaaatg tcgtgcgcgc cgagcggagc tgtcgtgcca
     4021 ttggccgaga aacaaaaaaa caggggatga gcttcgacct ttgtgtcgcc ctccgcgacg
     4081 gagcggatgg cgcgcgcgag gtgggggtcg atcgaggcca tgcgataggc cacgctgctg
     4141 agccaggcga gataggcccg gagccattcc ctcagtgtcg ggggcaggca gcccagacgg
     4201 tgttcgtggt agcgcgcggt gcgcttgtcc gtgacgacga acaatccggt ttcgacatcg
     4261 atgcaccggc gagtcagcgt ctcctcttgc aagctgcggc ctgcaaggat gaatttcagc
     4321 gcggcgtaga aatgtgttgc gaccaagttg tgggcctcat gcacaacttc gattgcgcat
     4381 cggcgaggca cgcctgtcat tgcggtccgc actatcgaca ggctgtcgcg aatgaaggcg
     4441 cgagccgtgg catctgggat gagtcgcaac gagccgacgt ccggcaacgg ctgcccgccg
     4501 cagtttcccg atagcccgac gcgcctgtag cactcgcttg cgattgaccg gattctcgct
     4561 gcgcgaagga ggcagtaatt caggttggcg gtcgtcccga gtgtgaaatc gacgcccatc
     4621 gcggcggcgt aggtttcatc ccctgtccgg gagaggacgt agcgacccca ggagaaggac
     4681 agtcgtttca gcgctggtcg gtgggatgtc agggacagct cacgaagcat caacttggtc
     4741 ttcttcccga ggtgcccctt ctcggaatcg aacagatcgg cgagtgtttt ccctccggtg
     4801 ctctgctgtc gtctcctcaa ctctgcgacg gtttcggacg gaagcggcac ctgcacgtaa
     4861 cggtccgatg acgatctggt aaacgaattg tcaggggtgt gccgtccgat tacctcgtcg
     4921 agattccatt caaggcatcc agcagcgaga ttgatccgga ttccagcccc tgattcggac
     4981 gtgagaggta cctggcagaa gctcgaaggc ataacgccaa gatgaagcgt cagcagagcg
     5041 ccgagcgctt cgtcctcctg tcctgtgcgc cagcggtcca agatcttcgg aacaatggcc
     5101 tcgagctcga acggttgtgc gtattcatac agggccggtg tgccggacag aacccggcaa
     5161 ccggcctttc cgatggtagc tttacgtcct ctcagggggt cggcaacaga ccgggcctgc
     5221 tgatcacttc ccacttcgga agccgcctcg tgcgcctcgc gtcgctcctc gcctgaagaa
     5281 tgcgaaatga cttgaacagc ccggcgagta acgaacgggt tgctgaactt tcgggaaagt
     5341 gcctcaacca gaactgccat gagtgcagct tgaggggtgc cgggtgggag actgtcccgc
     5401 cggagtcgaa gttcgtcggc cgacagctcc aggggctgtg caaaacggag gccctttccg
     5461 cgcatccagc gcgagagctc ggcggccaac gttctcccaa gaccgttgct ttcggaaagg
     5521 aggtggagtg caaggagtgc gcggacgctc cgcaggcagc gtcgttcagg gggcgggagg
     5581 gtcagacttg gttgtgtcag caaatcccgc agcgggcagg ccggcaggga tcggaggtag
     5641 gccagatcgc gaaccgcgtc tgctctgtct tggcagaggg tatcaagcag cttccgaagt
     5701 gcgtcgggca gctcagtgga gacgatgccg cggtccaagg catccaggtc tgccctgagt
     5761 tcatgcgcca aggccgccag cccgatgccg gagattggaa cggcaaaagt tcgaatgagg
     5821 agctggcgag cgtggcggag ttcgtccgac tgcgtttcgg cttccggatg gctggcggtg
     5881 gactggggtt cggcagggcg caagtagtcc tcgccttccg gcgatggcat gagagtgcaa
     5941 gggttggtgt catccatggt cagatctccc gaattgacca gaagggttga agggaaatgg
     6001 agatctgttg ccgcgatggg gcaacgtgga taacgacgac gcgatcagac gcccgccagc
     6061 tggagcgtca gcagcgaagg atcaatgcgg agttgagcga ggacggatga tgcgtgagtc
     6121 ggttcgtggc agcgacagac ggactgacgc tgcgatggca ccatgtgcag gtgcaggcgg
     6181 cctgaaggcc ttttgcggca tgaattgctc gatgccgtga ggggcggtcc taggggacct
     6241 tttgcgcaag tgatctgcgt gaatgtatgt tatacagaag tgcttgaatg ccgcaagaaa
     6301 gggatgtgcc ttgcggcagg cacgggtcat ttctggtgcg ataaatccat gtcgcaaaac
     6361 ctgatctgtt tgcccggatg tgacaaatcg gcgtggcgat cggtttggca aggcgtgcga
     6421 tgtcactaag tggcgaacag ataatctctt ttgcgacaga agtttacggt tccggtgatg
     6481 tgctgagcac gttgtggacc gattaagaac agcgggaaac aacggcctgc tttaccctcc
     6541 gaaggcaggg ggacgttcga atcgtctcgg gcgcgccata tttttcaatg ggttaggtct
     6601 tcgcggacct agcccattgt tctttgtgcc caccctgtgc ccattttgtg cccgcgtcgt
     6661 gcccgacgtt tttggcacaa gacgtactaa gtcaccactt cgacctttgc ccgccgtgcc
     6721 tcacgcggtg ttcgttgcgt cggggatgta ctcgaacaac tcgccaaccg tgcattcgaa
     6781 caatgcacac aagcgatcaa tggtttcgag atcaacccga tttgccgtct cgttgtagag
     6841 cagggtgatt gtgttacggt gcaagcccgt ttcacgggct acatcggcga tcttcagttt
     6901 gtgctcgccc atcaggcgtg agaggtggca gcgcaccatt gtcgtcccct aggactaaat
     6961 gtcgttcaga atgacaaaaa agccttgaca agggcgtttt gcgtcttaga atgtcattca
     7021 gggtggcaaa aagatcttct cgaagacttt ctgtcgttct gcatgattct aacgagtgga
     7081 gaaagccatg tcactgactt acctgaccac cgacgaactg gccggaagga tcaaatacga
     7141 cccgcgtacg attcgcgagc gccttaaaga cagcgtcctg ttggagggaa cgcactacat
     7201 ccgccccttt ggcggacgaa agatcctcta tatctgggag cacatcgagc gcgacatggg
     7261 ctgcacctcc gagcagtcgt ttgcgatccc aatggccaac ggaggcgtct gccatggcta
     7321 gcatccgtgc cagaaaggat aacggaatgc tcttcatcga cttccggtac caggacaagc
     7381 gttaccgcga acagaccgcg ctcggcgaca ccgcagccaa ccgcaagcgc ttgcagaagg
     7441 tgcttgatcg catcgaggcc gacatcgc
//
LOCUS       DAOQEW010000188         7451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3533_length_7451_cov_8.564630, whole genome shotgun sequence.
ACCESSION   DAOQEW010000188 DAOQEW010000000
VERSION     DAOQEW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_7451_cov_8.564630"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLS35_16010"
     CDS             <1..183
                     /locus_tag="PLS35_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20226.1"
                     /translation="YWEEGELVVKEFSGHEERLKVTPETKIEGVVAGRLKTGDKIVAQ
                     ITDDRKALSIKVQIPE"
     gene            complement(314..1033)
                     /locus_tag="PLS35_16015"
     CDS             complement(314..1033)
                     /locus_tag="PLS35_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20227.1"
                     /translation="MKPLRIITLLMLSLAVIMGVQCLTAEPAAAHNWLGDCVVDFDNT
                     FALTYIYGQARGNFGYPTGIDNTGELQPCNATHQACWTYRHRCFGNYINVEDMTYGHI
                     HLTFDSPSLLTPCFVDPGDGPGAGFGRTVGDNCVAADWIHEPRTLGTHGKDHWIKIWM
                     EDRVTHQPRVFDMPIIRVTGTEPIQFWFKKSNGSWWYWSNLAPNPWLVGGWVQDVVEV
                     RIRGAQAAKGPYFIGAFSILD"
     gene            complement(1088..1489)
                     /locus_tag="PLS35_16020"
     CDS             complement(1088..1489)
                     /locus_tag="PLS35_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20228.1"
                     /translation="MSSVSYRSLLAPAIPYVIVIPATILSGCVPPPPHQFVPGGPVGE
                     AGQTVPGPRQASDDEVRAMLAESSRHQSMPSERVDLNGLLTQISQATTFRQKQHLVDQ
                     YHHAALLLPEAERNQAFQRLRDLGNQGRMTP"
     gene            complement(1875..2003)
                     /locus_tag="PLS35_16025"
     CDS             complement(1875..2003)
                     /locus_tag="PLS35_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20229.1"
                     /translation="MHDAALWASWYVWLLAVPVLVIGTLIRDWLHRRHPVRLRKRS"
     gene            complement(2230..3141)
                     /locus_tag="PLS35_16030"
     CDS             complement(2230..3141)
                     /locus_tag="PLS35_16030"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNI20230.1"
                     /translation="MPATLQAFIAIFVLGALLRVSGLLGKIHAERLASFVFSISLPAT
                     ILVSLDHVTFTATAWRLPLAACLITVSMLLCAWQLARRLHLTRPTQGGLLLGTGCINS
                     VYFAYPVVLATFGDQGLARAILFDLGQTTLTLTLIYALAVGHGTKSTTARSAIVRFLS
                     SPPLWALCGSLLLSLFGLHLHDWLRQILTPLHLTTTPLASLVLGLSISVAVTHRTFAL
                     ATLGVVMRMAGGLLLGLGVAFVLNLTGIERAIVVLIAAMPSAVTAVIFAADTGLDEDL
                     VTSIVALSICVGIATLPWLPQLIRLLE"
     gene            3252..4478
                     /locus_tag="PLS35_16035"
     CDS             3252..4478
                     /locus_tag="PLS35_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNI20231.1"
                     /translation="MNQAQPSVPPRQAAVFFILVTVVLDMLSFGIIIPVLPKLVEEFL
                     GGDTAQAAEIYGLMGTSWALMQFVCSPIQGALSDRFGRRPVVLLSNLGLGLDFILMAM
                     APSLAWLFAGRVISGIASSSFSTAGAYIADVTAPEKRAAAFGLMGASFGLGFVLGPAV
                     GGLLGALDPRWPFWGAAATSLLNACYGFFVLPESLPVEKRAPFRWQRANPAGALILLR
                     SHHELFGLATANFLMNLAHGVLPSVAVLYLGYRYGWGPSAVGFTLAAVGICAMIVQGT
                     LVRPITAKLGERRTLLTGLLCGATGFAIYGLAPTPLIYCLGIPVMAFWGLAGPSAQVF
                     MTRRVSASEQGQLQGAIASLTGIAGLIGPTLFTQTFALFIGPQADWHMPGAPYLLSTM
                     LLLVSAATAWRATRPV"
     gene            complement(4538..5488)
                     /locus_tag="PLS35_16040"
     CDS             complement(4538..5488)
                     /locus_tag="PLS35_16040"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20232.1"
                     /translation="MIGFWFLLLWCLPAQAWAVSPGDVTPTTSPNQLSGRELLRIGEV
                     HDKQEHWPETLTYYQLALSKFREKKQQQGIATTLMKIARVHERQGKLQDAHASLREAE
                     QLFAQAADRSAHAEALLAMGRVAAQLGQRDESRDALTKAAALFTRVRNARGWNETMVQ
                     LGLLQVVEGSADAGLSSLQQAAEEAHTRRHVDQQFTATVALGDAHWLLGRLEEARTHY
                     LDALALAQAEHHLPFEGMLHLRLARLDQGKDTLNDRVALGKRAIQIAQAIHDTAAEAE
                     AWSLLADLHRQLGQQAEADTAEMRALALYRSRELFVHGVQ"
     gene            5743..6318
                     /locus_tag="PLS35_16045"
     CDS             5743..6318
                     /locus_tag="PLS35_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20233.1"
                     /translation="MAGATGTRQKKQAPQRKKAGPVKKTTRARAGQPSGAVIVLSGAT
                     PLRRQKAAEVLAEELQLHLAAVDLSAVVSKYARETEKNMTRVFDMANRHGSILFFDEA
                     DALFGKRTQVNDSHDRYANSEVSYLLQCIETHQGLVILATNGKSRIEDAFSPEKPVIV
                     LGGTAKKQAGVKPKPKAKSTPAPTKKVRKKK"
     gene            6419..6868
                     /locus_tag="PLS35_16050"
     CDS             6419..6868
                     /locus_tag="PLS35_16050"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HNI20234.1"
                     /translation="MDLPIRSPRQKLGEYVILPRLIDKVRLHAAGALPLDYVRNLLKP
                     GLTLDGRFLKFTGLAADALEGAILAAESDSLVLAWVDEQAIVHSDAEKQAWSEKAAAF
                     HPDPRVAKYLMMSLPDLATSLDITRYTLFDLIDMDEGRLPVPSRPKA"
     gene            complement(6904..7155)
                     /locus_tag="PLS35_16055"
     CDS             complement(6904..7155)
                     /locus_tag="PLS35_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNI20235.1"
                     /translation="MTWIVYILECADGSLYTGITNDLERRMRSHASGNGAKYTRRRGP
                     FTVRYTESLASKGAALQREAAIKALDRAAKVALITALQC"
     gene            complement(7247..>7451)
                     /locus_tag="PLS35_16060"
     CDS             complement(7247..>7451)
                     /locus_tag="PLS35_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20236.1"
                     /translation="TAARATKTSVRAEHSMMTQRLRKSSMYRFHDSVRHDRKYLIVAL
                     AAALILAAILASLGLTVFPTFPK"
BASE COUNT         1487 a         2057 c         2349 g         1558 t
ORIGIN      
        1 tattgggaag agggcgaact cgtcgtcaaa gaattctccg gtcacgaaga acgtctgaaa
       61 gtgacgcctg agacgaaaat tgagggcgtg gtggccggac gattaaaaac cggcgacaag
      121 atcgtggccc agatcaccga cgatcgcaag gcgctcagca tcaaggtgca aattcctgaa
      181 tgacggtcga gccgttccct tcaacagacc gtccagcgtg gccgcggcga gaaaagtggc
      241 cgatcgtgta tataccgcat ggtgagcctc tcagcgaagc cggaacgctg ccgctgggct
      301 gtatcaacag ccgttaatcc aggatcgaga acgcgccgat gaagtatggc cctttcgcgg
      361 cctgcgcccc cctgatccgc acttcgacca catcctgcac ccatccgccg acgagccagg
      421 gattgggtgc caggttcgac cagtaccacc agctgccgtt tgatttcttg aaccagaact
      481 gaatcggctc ggtgccggtc actcggatga ttggcatgtc gaagacgcgc ggctggtggg
      541 tcaccctatc ttccatccag attttgatcc agtgatcttt gccgtgcgtg ccgagtgttc
      601 gcggttcatg gatccaatcg gcggccacgc aattgtcacc gactgtccgt ccaaatcccg
      661 cgccggggcc atccccagga tcgacgaaac aaggcgtgag caggctcggg ctatcgaagg
      721 tcagatggat atgcccatag gtcatatcct cgacgttgat gtaattgccg aagcagcgat
      781 gacgataggt ccagcaggcc tgatgggtcg cattgcaggg ctgcaattcg cctgtattgt
      841 cgatgccggt gggatagccg aagttgcccc tcgcttgtcc gtagatatag gtcaaggcga
      901 atgtattgtc gaaatcgacg acgcaatctc cgagccagtt gtgagctgca gccggctcgg
      961 cagtgaggca ctgcacgccc atgatgactg ccaggctgag catcagcagc gtgatgattc
     1021 gtagcggctt catggtgacg tcctttcttg gctggatctt agtcgttgaa tgggcgtatc
     1081 cgctccctca cggtgtcatc ctgccttgat tccccaggtc tcgtagccgc tgaaaggctt
     1141 gattacgctc ggcttccggc agaagaaggg ctgcatggtg gtactgatcc accaaatgct
     1201 gcttctgccg gaaggtggtg gcttgagaaa tctgcgtgag tagtccgttg agatcgactc
     1261 gctcggaagg catcgattgg tgccgtgaag attccgccag catggcgcgc acttcgtcat
     1321 cgctggcttg tcgcggaccg ggcactgttt gtccggcttc cccgaccggg cctccaggca
     1381 cgaattgatg aggcggtgga gggacacagc cggacaggat cgtcgcgggt atgacgatca
     1441 catatgggat ggcgggtgcg aggagtgatc gatatgacac gctactcatg acggcctcct
     1501 cttaactgcc ttgcgtatgt cccgcaagct gcctggctca aagtcggtat ttttcgggca
     1561 cggcaaccag gcatgtttaa gatgctcttt ctgaaaacag gaaaggcaac ctttgggcca
     1621 cgacgtcaga gggcaccctt cgtacgtaat aacaagtgct ttgagctggg gcagctcgag
     1681 gagtcgcgct gcggcgtgca ggcgcgggta tcacatacga tacaacgtcg tatcccatcc
     1741 gatacgacta cgtagcttgc aacggatggt cgctgcctat cagagtgaaa tgaggttgat
     1801 ggagtgtggc gtgctgcgtg gggagtctga ctgtcctgag tcaaaccggc aggatcgagg
     1861 ccgatggttc tttactacga tcgcttgcgt aatcgcaccg gatgacgccg gtgtaaccag
     1921 tctcgaatga gcgtgccgat gaccagaacg ggaactgcca gcaaccatac ataccaactg
     1981 gcccagagcg cggcgtcatg catgatgtat cctccagtct atgtgccgat ggagagagca
     2041 agcaccatac cgtagaccgt ctttctgagg cgtcgtgccg tcgtcctgca aatctatagg
     2101 aaatctcacg agatagatga gtaacgcggt gtcgcgcttc gttacacgat gcggccggca
     2161 atttttgccg tcgcaaaaag ctcacaaggc gtgatgccgt tcccacagct gtcggcatgg
     2221 ctgagagtct tattccaaca agcggatcag ctgcgggagc catggaagcg tcgcgatccc
     2281 cacgcaaatc gagagtgcca cgatggaggt gaccaggtct tcgtccagcc ctgtatctgc
     2341 cgcaaagatc acggcggtga cggcggaggg catcgcggca atcagcacga cgatggctcg
     2401 ttcgatcccg gtgaggttca ggacaaaggc gacacccaat ccgagcagca gcccgcccgc
     2461 catgcgcatg acgacgccga gcgtggccaa ggcaaaggtg cggtgcgtga cggcgacgct
     2521 gatcgagagt cccagcacga ggctggccaa aggcgtcgtc gtgagatgaa gaggggtgag
     2581 gatctggcgg agccaatcgt gaagatgcag cccgaagagt gatagcagga ggctcccgca
     2641 gagggcccac aatggcggtg atgacaggaa tcggacgatt gccgagcgag ccgtggtgct
     2701 cttggtgccg tgtccgacgg cgagggcgta aatcagcgtg agggtcaggg tagtctggcc
     2761 aaggtcaaac agaatggcgc gcgccaatcc ttggtcgccg aatgtggcaa ggacgacggg
     2821 gtaggcgaag tagacggaat tgatgcagcc ggtgccgagt aataaacctc cctgcgtcgg
     2881 acgtgtcaga tgcagcctgc gggcaagttg ccaggcacag agcagcatgg agacggtgat
     2941 caggcaggcc gcgaggggca atctccaggc cgtcgcggta aacgtgacat gatccaggga
     3001 gacaagaatt gtagcgggca gactgattga gaagacgaag gaggcgagcc gttccgcatg
     3061 tatttttccg agcagtccgg atacacggag caacgcgcct agaacaaaaa tggcgatgaa
     3121 ggcttggagc gtggcgggca tcggcggcag cctagcagaa aatcgttggc aagaggaggc
     3181 atggctcggc atgaagcatc ccgccttttt ctgtgcggcg aaggcgcgag cctggtacga
     3241 tggcgcactt catgaaccag gcgcagccat cagtgccacc gcggcaagcg gcggtctttt
     3301 ttatcctcgt caccgtcgtg ctcgacatgt tgtcgttcgg gatcatcatt cccgtgctgc
     3361 cgaagctggt cgaagagttt ctgggcggcg acacggcgca ggcggcggaa atttatggtc
     3421 tcatgggcac gtcctgggcg ctgatgcagt ttgtctgttc accgattcag ggcgcgttat
     3481 cggatcggtt cgggcgtcgg cctgtcgtcc tcctgtccaa tcttggcctc ggccttgatt
     3541 tcatcctcat ggcaatggcg cccagcctgg catggttgtt cgccggtcga gtcatctccg
     3601 gcatcgcgtc atcgagtttc agtactgccg gcgcgtatat cgccgatgtg accgcgccgg
     3661 agaagcgggc cgctgcgttc ggcctgatgg gcgcatcctt cgggttgggc ttcgtgctgg
     3721 ggccggccgt cggcggtctg ctgggtgccc tcgacccacg ctggcccttc tggggagctg
     3781 cggcaacgag tctgctcaat gcctgttacg gattcttcgt cttgccggag tcgcttcccg
     3841 tggagaagcg ggcgccgttt cgttggcagc gggcgaatcc ggccggcgcg ttgatcctct
     3901 tgcgctccca tcacgaactg ttcggtctgg caacggcgaa ctttctcatg aatctggcgc
     3961 atggcgtact gcccagcgtg gccgtgttgt atttggggta ccggtacggc tggggtccgt
     4021 cggcggtcgg cttcacgctg gcggcagtcg ggatatgcgc catgatcgtc caaggcacct
     4081 tggtgcggcc catcacggcg aagctgggag aacgccgcac attgctcacg gggctcctgt
     4141 gcggcgcgac cggctttgcc atttacggcc ttgcgcccac gccgttgatc tattgcctgg
     4201 gtattccggt gatggcgttt tggggacttg ccggcccttc cgcgcaggtg ttcatgacgc
     4261 gccgggtgag cgcgtctgag caggggcaac tgcagggggc gattgccagc ctcaccggca
     4321 ttgccggatt gatcggcccg acattgttca cgcagacgtt tgctctgttc atcggcccac
     4381 aggccgactg gcatatgccc ggtgcgccgt atcttctgtc gacgatgctg ctgctggtga
     4441 gtgcggctac tgcgtggcgc gcgaccaggc cggtgtaagc gatttgggta ttgctgacag
     4501 cgatcaggtt tggacaggtg gtcgagcagg ccttcgtcta ctgcacgccg tgcacgaaga
     4561 gttcccggct gcgatagaga gccagtgccc gcatctctgc tgtatctgct tccgcttgct
     4621 ggcctaattg ccggtgaagg tctgcgagga gcgaccaggc ttccgcttcg gcggcggtat
     4681 cgtgtatcgc ctgagcgatt tgaatggcgc gttttcccag cgccacgcgg tcattcagcg
     4741 tgtctttccc ttgatcgagc cgggcgagcc gcaggtgcag catgccttcg aacggcagat
     4801 ggtgttccgc ctgggccagc gccagtgcat ccaggtagtg tgtgcgtgcc tcttccagtc
     4861 gccccagcag ccaatgcgcg tccccgagcg cgacggtcgc ggtgaactgc tgatcgacat
     4921 ggcgccgagt gtgcgcctcc tccgccgcct gctgcaagga cgagagcccg gcatcggccg
     4981 agccttcgac gacttgaagc aggcccaact gcaccatcgt ctcgttccat cctcttgcat
     5041 tcctcacgcg ggtaaagagg gcggcggctt tcgtcagggc gtcgcgtgac tcatcacgtt
     5101 gccccagttg tgcagccacg cggcccatcg ccagcagggc ttcggcatgg gcggatcgat
     5161 cagcagcctg tgcaaataac tgttcggctt cccgcaagga cgcatgggcg tcttgcagtt
     5221 tgccttgtcg ctcgtgcacg cgcgcaatct tcatgagcgt ggtcgcaatg ccctgctgtt
     5281 gcttcttctc ccggaacttc gacaaggcga gttggtaata ggtcaacgtt tcgggccaat
     5341 gttcctgttt gtcgtggacc tcgccgatgc gaagcaattc acggcctgag agttggttcg
     5401 gcgaggtggt aggcgtgacg tctcccggcg aaacggccca ggcttgggca ggcaggcacc
     5461 agagcaagag gaaccaaaac ccgatcatgc tgtctctcca tcaagctgca gagtgggggc
     5521 ctacgaggaa gtgatctgtc gcgtactttg cattgacgtc ccggtaaggt caagccagta
     5581 agctgaggtc gtgttccttt tctcatgttt ccgcgcatcg accctatgct ttcgacgatg
     5641 aagaaattca gcgacaaatc caggaaccgg ttatactcag cgcctgtttg tctgacggaa
     5701 gcgtggcaag ggctcacttg gaggcggaaa ggaatgaaaa ccatggcagg tgcaacggga
     5761 acccggcaaa agaaacaggc tccgcaacga aaaaaggccg ggcctgtgaa gaagaccacc
     5821 agggcgcgtg ccggccaacc atccggtgcg gtgatcgtcc tgtccggagc cactccgctt
     5881 cgtcggcaaa aggcggctga ggttctggcg gaagagttgc aattgcatct ggccgcggtc
     5941 gatttgtcgg ccgtggtgag caagtatgcc cgcgagaccg aaaaaaacat gacacgcgtg
     6001 ttcgatatgg ccaatcgcca cggatcgatt ctcttctttg acgaggccga tgccttgttc
     6061 ggaaaacgga cgcaggtcaa tgactcccac gatcggtatg cgaacagtga agtgtcgtat
     6121 ctcctgcagt gcatcgagac acaccaggga ctcgtcattc tggcgaccaa cgggaagagc
     6181 cgtatcgagg acgccttctc tccggaaaag ccggtcattg tgttgggcgg gaccgcgaag
     6241 aagcaggctg gcgtcaagcc gaagccgaaa gccaagtcaa cgccagcgcc taccaagaag
     6301 gtgagaaaga agaaataagc cagaccggtg aggggggtgg cgcgcccatt ccaactcggc
     6361 caaccagaat tgtttcctga gaactgttga aagcttgaag aagggggcgc tcgggcgcat
     6421 ggaccttccg atacgatccc ctcgtcaaaa gctcggcgag tatgtgatcc tgcccaggct
     6481 tatcgataag gttcggcttc atgccgcagg tgctttgccg ttggactacg tgcggaattt
     6541 gttaaagcca ggacttactc ttgatggcag attcttgaaa ttcaccggac ttgccgcgga
     6601 cgcgctggaa ggtgcgattc tagcggctga atcagactca cttgttttgg catgggtgga
     6661 tgaacaggcg atagttcatt cagatgcaga gaaacaggcg tggtctgaga aggcggcggc
     6721 gtttcatccg gaccctcgtg tggctaaata tctgatgatg agccttccag atcttgcaac
     6781 tagcctggat atcacccgtt atactctctt tgatttgatt gatatggatg aggggcgttt
     6841 gccggttcca tcgaggccga aggcctgaac caagcagaca cgctgtacga ggtcgctgtc
     6901 gtttcagcac tgcagggccg tgatcagcgc gaccttcgcc gctcgatcca gcgccttgat
     6961 ggcggcctcg cgttgcagcg cggcgccttt gctagccagt gattcggtat accgcaccgt
     7021 aaacggtcct cggcgtctcg tgtatttcgc cccatttccg cttgcatgcg atcgcatgcg
     7081 acgctccaaa tcgtttgtga tgcctgtata caggctgccg tcagcacatt ccagaatata
     7141 gacgatccac gtcatgagca gcgaggcctg gtatatggat gcgcttccga cgcacaggtg
     7201 tgtgccggac tcacgcgatc atagccgaaa cgtctctaag aggcgactat ttggggaagg
     7261 tggggaagac cgtcagtccg aggcttgcga ggatggcggc gagaatgagc gctgcagcca
     7321 gtgcaacgat cagatacttc cgatcatggc gcaccgaatc gtgaaagcgg tacatgctgg
     7381 atttgcgcaa acgctgagtc atcatgctgt gttcagccct gacagaggtc tttgtggcac
     7441 gtgctgccgt c
//
LOCUS       DAOQEW010000189         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3645_length_7318_cov_6.190279, whole genome shotgun sequence.
ACCESSION   DAOQEW010000189 DAOQEW010000000
VERSION     DAOQEW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_7318_cov_6.190279"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PLS35_16065"
     CDS             <1..906
                     /locus_tag="PLS35_16065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20237.1"
                     /translation="GLRFVALSLFRRVLLLHVFLASRASFWRKSIFLSWAALLNIPVV
                     IHLHGGEFHVFFEKECGPRRQRFIRWIFGQAKVVIVLSEAWRSWVQGQIPLARVRVMA
                     NPTRLPVATSNEAREGCTLLFLSRVVAAKGIFELIQALAKLVDDFPALKLKVGGDGDL
                     TAASRLADSLGVGGRIEFLGWVRGDSKTKLLTTATIYVLPSYNEGLPMGVLEAMSAGM
                     AIVASRVGGIPEAIEDGREGFLVAPGNVEQLVLCLHRLLLDGDLRVNIGAAARRKAVT
                     HFDVNNIVLRLEELYADILKGREVA"
     gene            979..3198
                     /locus_tag="PLS35_16070"
     CDS             979..3198
                     /locus_tag="PLS35_16070"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HNI20238.1"
                     /translation="MVRQCTWILLWLSAVVHADDSSMIPPGAMPLRPEDHAEVLVNPP
                     IMTWPLLRRGPYVVEIIGPDGESRKHSVARNWLLLPQVLRSGTYRWKVSSPNDTLVDW
                     RQFRIPSTARTWLVPPAQELLKKSIERARPRSVDVVELQASVDPSVRELLLKAVRRAE
                     DLPLWMESEKVSVQRNQEDVLADQRELRKRSVDEEALLLRAATAWLTQRDELALALAK
                     RRALALASLDPLGATSFDNHDQAGRAVAWSLALIFDWLHDQFSEDERRKLLDAIEPRV
                     EQILGKGPFGIDRSLRLDVRPYDSHGVTALSRVAVICALLAGERPIFARCFSQVVPRY
                     VALPIPWGEDDGGFSNGTAYAHWAVLDNHLLAWDFLGRMLGLNFYALPWVENYARMVA
                     YFLPPGAPSGVFGDEAEYYHRQVWANQALALAARMSTSLTRWYARQFFREDPLHPALL
                     LAHRVQKSGSPPEDADGGWSGNAAMLPSVGWAAMHSDLRDRERISIYFKSSPFGSFNH
                     SHADQNGFVVHARGVPVIIDSGYYDYYGSPHWHAWYKQTRAHNAVTFDGGQGQAIDSF
                     AAKGRIVHYEHRRDLDVVSGEAAPAYGSQVKTALRTLIYIRPSTLVVVDNLESVVGRT
                     WEWNLHAAERFEVGRERRFALDSKAGRLCGRMIDDSVGQFSQSDRFDKMPSTGKPNQW
                     HLRYSTSSRVKSVQFVAVFDIDCERSTATVGQVQDALTVTAGKAVVKIHSNGRVKVL"
     gene            3198..5186
                     /locus_tag="PLS35_16075"
     CDS             3198..5186
                     /locus_tag="PLS35_16075"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNI20239.1"
                     /translation="MTKWKWMLNRLQTMSAQEMAWRGWQAVTRKAFVMGIGRADDPPE
                     PDEGRCGAGFLSVDALDRIDRRPIVEKADAILAGRWDIFALQDVQLGFPPRWNVDPLS
                     ATQAPLVCGKAIDFRQRELVGNIKYLWEPSRHLELVTLAQAWLLSRDDRYLNGVRTLL
                     DSWLDECPYPTGVHWASSLELAIRLVNWACCWQLVGGASSRLFEGSSGAAFRKRWLRS
                     IYQHCHFVRNDLSLHSSANNHLFGEYMGLFVASLMWPCWEDSRAWFDFSMRGLEEEAV
                     RQISMDGVHREQAIYYQHEVMSMMLISIRSAAASGVQLSDGFLARLERMAEFVASLMD
                     VGGNVPMIGDADGAQMIRLEQDREHDVYRSLLVGCAILFGRADFKRFARQLDDCNRWY
                     FGATGDAVWNDLDACSAETGPALFPDGGYFFFGKDFGEGSEIKGIVDCGPIGYPSIAA
                     HGHADALSIMLSIAGQPCLIDPGTYSYWADEVWRSYFRGTSAHNTIRIDEVDQSESGG
                     RFMWLRKARVRVHEVAKVGDAFRFVGAHDGYERLDDPVRHTRVVEFDAVSSSMTVVDE
                     LTGMSSHIIEQFWHFAPGIEVSVRNGVVLARGQRFLVKAVFDTVTVSLDVWRGSEDPL
                     LGWYSDAYERKMPCTTLRSRVTAAATRLKVVFSIELYV"
     gene            5210..6319
                     /gene="wecB"
                     /locus_tag="PLS35_16080"
     CDS             5210..6319
                     /gene="wecB"
                     /locus_tag="PLS35_16080"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNI20240.1"
                     /translation="MPCIYLVAGARPNFMKIAPIVRALHKSGRLDYRIIHTGQHYDRD
                     MNEVFFEELGIPAPDVFMAAGGGSHAQQTAKIMMAFEDYCRENPPDAVLVVGDVNSTL
                     ACSIVAKKLCIPVAHVEAGLRSGDMSMPEEVNRLVTDSISDWFFVTEPSGVEHLLREG
                     KPTSAIHHVGHVMADNVLYQAEQLDRSACPDDEAAKLKATLLRDFGRYGVVTLHRPSN
                     VDDPVVFAKLAAALRKVSLDIPLVFPVHPRTRANMERFGIDLGPNVALIGPQAYMAFL
                     NVWKDAFVVLTDSGGLQEETTALGVPCITLRTNTERPVTVDEGTNVLAGVETEAIVAE
                     VDRVLRGQGKRGQRPALWDGHAADRIVAVLVSELT"
     gene            6316..7053
                     /locus_tag="PLS35_16085"
     CDS             6316..7053
                     /locus_tag="PLS35_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNI20241.1"
                     /translation="MSKRIEMMGCQIDNLSMEETLQTVEGFIRTGLPHQHVVVNVDKL
                     VKASRDPELKRIINDCALINVDGMPVVWASRLLGKGLKERVAGVDLFEALMERSAQRG
                     WRVFLLGAREDVVSGVKQLYEKKYAGLTVAGYRNGYWKPHEEADVVRQIQEARADLLF
                     VAISSPKKEQFLGRYQAEMKVPFAMGVGGTFDVAVGKVKRAPVWMQKAGLEWFYRFLQ
                     EPRRMFRRYFVDDMAFFWLLLKEVGSR"
BASE COUNT         1394 a         1828 c         2376 g         1720 t
ORIGIN      
        1 ggccttcggt tcgtcgccct gtcgctattc agacgggttc tgctgctgca tgttttcctt
       61 gcgtcccggg ccagcttctg gaggaaatcc atattcctgt catgggccgc attgttgaat
      121 attccagtgg tgatccattt gcatggcggc gaatttcacg tttttttcga aaaggagtgc
      181 ggtcctcgcc gtcagcgctt catccggtgg attttcggac aggcgaaggt cgtcatcgtg
      241 ctgtccgagg cctggcggtc ctgggtgcag ggacagattc cgttggcgcg ggtcagagtc
      301 atggcaaacc cgacaaggct gcctgttgcc acgtcgaatg aggcccgtga gggttgcaca
      361 cttctatttt tgagccgcgt cgttgcagca aagggaatat tcgaactgat tcaggcactc
      421 gcaaagcttg tcgacgattt tccggcgctg aagttgaagg tgggtggaga cggggacctg
      481 acagcggcaa gcagactggc agatagcctg ggtgtcgggg ggcgcatcga gttccttggg
      541 tgggttcgcg gagattcgaa gaccaagttg ctgacgacgg ccaccatcta tgtattgcct
      601 tcctacaacg agggcctgcc aatgggcgtg ctcgaggcga tgtcggctgg catggccatc
      661 gtcgcgtccc gcgtcggcgg tattccggaa gccatcgaag acggacgaga gggtttcctg
      721 gttgcgccgg gtaatgtcga gcaactcgtg ctgtgcctgc acaggctgct actggatgga
      781 gatcttcgcg taaatatcgg agccgctgca agaaggaagg cagtgaccca cttcgacgtg
      841 aacaacatcg tccttcgcct ggaagagctt tacgcggata tcctgaaggg ccgggaggtg
      901 gcctgattga ccgccgtacg atttgccgtg tcgtgggcgg aatggcacgc tctcttcaaa
      961 ttccgagcat ggcaggccat ggtcaggcag tgcacatgga ttctgttgtg gctttcagcg
     1021 gtggtacatg ccgacgattc atcgatgatt cctcccggtg cgatgcctct tcggccggag
     1081 gaccacgcgg aagtactcgt caatccaccg atcatgactt ggccattgct gcggcgtggg
     1141 ccctatgtgg tcgagatcat cggacccgat ggagagagtc gtaaacattc ggtagccaga
     1201 aactggcttt tgctgccgca ggtattgaga agcgggacgt atcggtggaa ggtcagtagc
     1261 ccaaatgaca cgttggtcga ctggcgtcag ttccgaatcc cgtctaccgc tcgtacgtgg
     1321 cttgtgccgc cggcgcagga gttgctcaag aaatccatcg aacgcgctcg tccccgctcg
     1381 gtcgacgtcg tcgaactcca ggcctccgtg gatccatcgg tccgtgagtt gctgctgaag
     1441 gctgttcgcc gggccgaaga ccttccactg tggatggagt cggagaaggt atcggtccag
     1501 agaaaccaag aggatgtact tgcagatcaa cgggagttgc gaaagcgcag cgtcgatgaa
     1561 gaagcgctgc tccttcgcgc agccacggct tggttgacgc aacgcgatga gctcgcgttg
     1621 gcgctggcga agcggcgcgc gctggcgctc gcatccctgg atccgctcgg tgcgaccagc
     1681 ttcgacaatc acgatcaggc tggtcgtgcg gtcgcctgga gtctggctct gatattcgat
     1741 tggttgcacg atcaattttc ggaggacgag cgccgaaagc tcctggacgc tatcgagccg
     1801 agggtcgaac agatcctggg gaaggggcca ttcggtatcg accggtcgct tcgcctggat
     1861 gtgcgtccct atgattccca cggtgttacc gcgctcagca gggtcgccgt catctgcgct
     1921 ctgctggctg gggagcgccc aatctttgca cgttgtttca gtcaggtcgt cccccgctac
     1981 gtcgcgctgc ccattccttg gggggaggac gatggcggat tttccaatgg gaccgcctat
     2041 gcccattggg cagtgctgga taaccatcta ctggcttggg attttcttgg gcggatgctc
     2101 ggcttgaatt tctatgcgct gccctgggta gagaactatg caaggatggt tgcatacttc
     2161 cttccgcccg gggcgccttc cggagtgttc ggcgacgagg cggagtacta tcacaggcag
     2221 gtgtgggcca atcaggcact ggccctcgcg gcaagaatgt cgacgtcttt gactcgctgg
     2281 tacgcccggc aattctttcg cgaggaccca ttgcatccgg ccctgctgct ggcacatcga
     2341 gtccagaaga gtggctcgcc cccggaagat gcggacggag gctggagcgg aaacgctgcc
     2401 atgttgcctt ccgttggatg ggccgcgatg cacagtgatc tcagggaccg cgagaggata
     2461 tccatctact tcaagtccag tccgttcgga tcattcaacc attctcatgc ggaccagaac
     2521 ggtttcgtcg ttcatgctcg tggtgtcccg gtgatcatcg actccgggta ttacgattat
     2581 tatggctctc cccattggca tgcttggtac aagcagacgc gagcacacaa cgcggtgacc
     2641 tttgacggtg ggcaagggca ggcaatcgat tcttttgctg caaagggtcg aattgttcac
     2701 tatgagcatc gtcgcgatct ggatgtcgtc agcggcgagg ccgcgccggc ctatggatct
     2761 caggtgaaga ctgccttgcg gactttgatt tatattcgtc caagtacctt ggtggtggtc
     2821 gataatctcg agtcggtagt ggggcgtacg tgggagtgga atctgcatgc tgcggagcgc
     2881 ttcgaagtgg ggcgcgaacg gcgatttgcg ctcgacagca aggccggtag actttgtggc
     2941 cgcatgatcg atgattccgt gggtcagttc agtcagagcg atcggttcga caagatgcct
     3001 tcgaccggaa agccaaatca atggcatctg cggtattcga caagcagtag agtgaaaagt
     3061 gtgcagttcg tcgcggtctt cgacattgac tgcgaaaggt caactgccac tgtcggacaa
     3121 gttcaggatg cgctgactgt cacagccggc aaagcggtcg tgaaaatcca ttcaaatggg
     3181 cgggtcaagg ttctgtgatg acgaagtgga aatggatgct caaccgactg cagacgatgt
     3241 cggcccagga gatggcctgg cgtggctggc aggcggtcac ccgcaaagcg ttcgtgatgg
     3301 gaatcggtcg ggccgacgac cctccagagc ccgacgaagg ccgctgcggt gcaggcttcc
     3361 tttcggtcga tgccctcgat cggatcgatc ggcgccccat cgtcgagaag gccgacgcca
     3421 ttcttgcggg gcgatgggat atctttgcgc tgcaggatgt acagcttgga tttccaccgc
     3481 gatggaacgt cgacccattg tcggcaaccc aggctccgtt ggtgtgcggt aaggcgatcg
     3541 acttcaggca aagggagctg gttggaaaca tcaaatacct gtgggagccc agtcggcatc
     3601 tggagttggt gaccctggcg caggcctggc tgctgtcgcg ggatgaccgc tatctgaatg
     3661 gagtgcggac gctcctggat tcctggctcg atgaatgccc gtatcccact ggtgttcatt
     3721 gggcgagctc gctggagctg gccattcggt tggtcaactg ggcttgctgc tggcagctcg
     3781 tcggcggtgc gagttcgagg ttgttcgaag gttccagtgg cgcggcattt cgcaagcggt
     3841 ggctgcgctc gatctatcag cactgccatt tcgtcaggaa tgatctgtcc ttgcattcat
     3901 ctgccaacaa ccacctgttc ggtgagtaca tggggctgtt cgttgccagt ctcatgtggc
     3961 cctgctggga ggattcacgc gcctggttcg atttttccat gagaggactc gaagaagaag
     4021 cggtcagaca gatttcgatg gatggggtcc atcgcgagca ggcgatctat tatcagcacg
     4081 aggtcatgtc gatgatgctg atatcaattc gttctgctgc agcaagtggg gtgcaattat
     4141 cggacggttt tctggctagg ctggagcgta tggccgaatt tgtggcatcc ctgatggacg
     4201 tcggtggcaa tgtcccgatg atcggtgatg cggatggcgc tcaaatgatt cggctcgagc
     4261 aggatcgaga gcacgatgtg tatcgatcgt tgctggtcgg ttgcgccatc ctgtttggcc
     4321 gggccgattt caagcgattc gctcgacaat tggatgactg caacagatgg tacttcggtg
     4381 cgaccggcga tgcggtctgg aatgatctgg atgcttgctc tgccgaaaca ggccctgcct
     4441 tgtttcccga tggcggatat tttttctttg gtaaggattt cggcgagggt tcggaaatca
     4501 aaggtattgt ggactgtggt ccgatcggct atccaagcat tgccgcgcat gggcatgccg
     4561 atgcgctttc gatcatgttg tcgattgccg gacagccttg cctgatcgac cccggtacat
     4621 actcctattg ggccgatgag gtttggcgat cttacttccg aggcacctcc gcacacaata
     4681 cgattcgaat cgatgaagtg gaccaatcgg agagcggcgg acgcttcatg tggcttcgca
     4741 aagccagagt tcgcgtgcat gaagtcgcga aagtgggcga tgccttcagg tttgtgggtg
     4801 ctcatgatgg ctacgagcgt ctcgacgatc cagttcgtca cacccgagtt gtggagtttg
     4861 acgctgtaag ctcgtcgatg acggtcgtcg atgaactgac cgggatgtcc agtcatataa
     4921 tcgagcagtt ttggcacttt gcgccgggta tcgaagtttc ggtacgaaat ggggtggttc
     4981 tggcgcgggg acaaaggttt cttgtgaaag cggtattcga taccgtgacc gtgtcgctcg
     5041 atgtttggcg tggcagtgag gatccgctgt tggggtggta ctcggatgcc tatgagcgca
     5101 agatgccatg tacgacgctt cggtcgcggg tgactgccgc tgctacccgt ctgaaggtcg
     5161 tgttctccat cgagttgtat gtgtgaatga gatttggaag gaaacttgaa tgccttgtat
     5221 ttatctggtc gccggagcac gtcctaattt catgaagatt gcgcctattg tgcgtgcgtt
     5281 gcacaagtca ggtcgactcg actatcgaat cattcatacc ggccagcatt acgatcgcga
     5341 catgaatgag gtgttcttcg aggagttggg aattccggct cccgatgtct ttatggcggc
     5401 cggtggtgga agccatgcac agcagaccgc gaagatcatg atggcgttcg aggactactg
     5461 tcgggagaat cctcctgacg ctgtgcttgt ggttggggat gtcaactcca cgctcgcctg
     5521 ttcgatcgtt gcgaagaagc tgtgcattcc ggttgcgcat gtggaggcgg ggctgcggag
     5581 tggcgatatg tcgatgccgg aggaggtcaa tcggctcgtc accgacagca tttcggactg
     5641 gttctttgtg acggaaccga gtggcgtcga gcacctcctc cgtgagggca agccgacgtc
     5701 cgcgattcat catgtcggcc atgtgatggc ggataacgtt ctttatcagg cggagcaact
     5761 ggatcgctct gcgtgcccgg acgatgaggc ggccaagttg aaggcgacat tgctgcggga
     5821 ttttggtcga tatggggtcg tgacgctcca ccggccgagc aatgtggatg acccggtggt
     5881 gttcgccaag ttggctgcag cattgcggaa ggtatctttg gatattccgc tggtctttcc
     5941 ggtgcacccc aggacccgcg cgaacatgga gcgatttgga atcgatctcg gtccgaacgt
     6001 ggccctcatc gggccgcagg cctatatggc cttcttgaat gtctggaagg acgcgttcgt
     6061 tgttctgacg gacagtggtg gacttcagga ggaaaccacg gcgctgggtg tgccgtgcat
     6121 caccttgcgg acgaacaccg aacgtccggt gacggtggac gaaggtacga acgtgctggc
     6181 aggtgtcgag acggaggcca tcgtcgcgga ggttgatcgg gtgctgcgtg ggcagggcaa
     6241 gcggggtcaa cgccccgcgc tctgggacgg gcatgctgcc gatcgaatcg tggcagtcct
     6301 ggtgtcggag ttgacatgag caagcgcatc gaaatgatgg gttgtcaaat cgacaatctg
     6361 agcatggaag agactcttca gacggtcgag ggctttattc ggaccggctt gcctcaccag
     6421 catgtggttg tgaatgtgga caagttggtc aaggcaagtc gtgatcccga gttgaagaga
     6481 atcatcaacg actgtgcact gatcaatgtg gacggaatgc cggttgtgtg ggcatcgcgt
     6541 ttgctcggaa agggtctgaa ggagcgcgtg gccggagtgg atctgttcga agcgctgatg
     6601 gagcgttccg cccaaagggg ttggcgggtc tttctactcg gcgcccggga ggatgtggtt
     6661 tctggcgtca agcagctgta cgagaagaag tacgctggac tcacggtggc tgggtatcgc
     6721 aacggctact ggaagccaca cgaagaagcc gatgtcgttc gacagattca ggaggcgcgg
     6781 gccgatcttc tttttgtggc gatcagctcg ccgaaaaagg agcagttcct ggggcgctac
     6841 caagcggaaa tgaaggtgcc attcgcgatg ggagttggcg gtaccttcga tgtcgctgtc
     6901 ggcaaggtga agcgcgctcc ggtgtggatg caaaaggcag gactcgagtg gttctatcgt
     6961 tttctgcagg agccgcgtcg aatgttccgt cgatattttg tcgatgatat ggcctttttt
     7021 tggctgctac tcaaggaagt cggttccagg taagttgttg atggtgagtt gcatgtttga
     7081 ctcaccgttt cgtgcggctt gaacggattg gggtgctatt cgaaatgggt cgaatacatc
     7141 ccatttttgt ttctacgacg taagaaaaaa ggccaaccgc gtgaagcggt tggccatgtt
     7201 cgtgtggtcg gagcttgaag gatcgatttt cgatcagcgt ttcaacgcct ccagggggaa
     7261 tcggcgcgca ggaacaatgc gcttgtgcct gccgtgacta ccgctggcaa cgggactg
//
LOCUS       DAOQEW010000190         7186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3752_length_7186_cov_6.993970, whole genome shotgun sequence.
ACCESSION   DAOQEW010000190 DAOQEW010000000
VERSION     DAOQEW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3752_length_7186_cov_6.993970"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLS35_16090"
     CDS             complement(<1..387)
                     /locus_tag="PLS35_16090"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNI20242.1"
                     /translation="MRIERRFTKRGQSPYEGLAFVKRSSEIRNPDGSTVFKLEHIDIP
                     EHWTQLAIDILAQKYFRKAGVPQVHEDGTPVVDAAGKPVLGGERDSRQVFNRLAGCWT
                     FWGKNHGYFKTPEDATAFHDEMCYMLA"
     gene            complement(716..1087)
                     /locus_tag="PLS35_16095"
     CDS             complement(716..1087)
                     /locus_tag="PLS35_16095"
                     /inference="COORDINATES: protein motif:HMM:NF025629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4359 domain-containing protein"
                     /protein_id="HNI20243.1"
                     /translation="MRTGTLVGLVGSLMLAVWLAATNPTMDTYLQFVEARLSAEIDKM
                     DQSGPRRDRDLIRSIFRAQGPKLVEGVVRPNTRRNNWGLLSLFETNVLDQPVLVLGIG
                     GQFVPLRGVEEATVKVGRLAF"
     gene            1244..1534
                     /locus_tag="PLS35_16100"
     CDS             1244..1534
                     /locus_tag="PLS35_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20244.1"
                     /translation="MFVWSKRLVTGLLAGFVLLIGIFLLQGEPETISGLKIHTTRWMA
                     VNYIGLLVWLFVWMFDQARVRGKNVFFWLVPFALAPLPTLMLFILFLQRKMK"
     gene            complement(1575..2840)
                     /locus_tag="PLS35_16105"
     CDS             complement(1575..2840)
                     /locus_tag="PLS35_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI20245.1"
                     /translation="MSHSSIRSYLTPRLGIMLPLGFASGLPLALTGGTLQAWLTEAGL
                     DLTTIGLFAYVGLPYTLKFLWAPVMDRIVPPWLGRRRGWMLVTQSGLALALTVMALIG
                     PAEGAQVFAALALAVAFLSASQDIVFDAYRTDLLKPEERGLGAATWVMGYRIAMIASG
                     SLALILAARLNWSAAYLCMSALMALGIVTILLSPEPEDAQVAPKSMAEAVWGPLSEFL
                     SRPMALALLGLIVLYKLGDAFAGALTTSFLLRGMQFSSEDIGVVRAFGMGATILGAFI
                     GGGLMPRLGLFRSLFVFGVLQALSNLSFMWLAWAGKSYAVMAFAVGAENLTGGMGTAA
                     FVALVMSLCNHRYTATQFALLSSVEALGRVFLGWPAAKLVGLAGWGPFFFVTFLAALP
                     GLWLLWVLRKPVAQHAELNAGRSAAGAPC"
     gene            3066..3470
                     /locus_tag="PLS35_16110"
     CDS             3066..3470
                     /locus_tag="PLS35_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20246.1"
                     /translation="MNVTNLGFTWNSRYLELSPTEMAIALVAALLVGGAALYFLLKVL
                     RSKPVKVLGMALLVAGIALVGWLALDQHQQDERARVSEIWSRTFENSAIKDFESCVAN
                     RTPRSPGPQYRVEVVTECGDVAKAIKEGLEAR"
     gene            complement(3535..4380)
                     /locus_tag="PLS35_16115"
     CDS             complement(3535..4380)
                     /locus_tag="PLS35_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNI20247.1"
                     /translation="MTAEPVPSATGSVADAYPPPEIAARVCKLGLAKVNTAVPTMVAL
                     AVLAGAFISLGALFYTVTITAGQDGSPLPFGLLRLTGGVTFSLGLILVVVGGAELFTG
                     NNLIAMAWAAGCVQTRQVVRNWIWVYLGNLLGACGTAGLVLLAGIQLLGDGAVGEAMV
                     RIARSKIALDPLSAFARGILCNVLVCLAVWLCMGARSVADKILAIVFPISAFVACGFE
                     HSVANMFFLPLGIALAAGSAAPLSLIDALSNLALVTLGNIIGGTVLVALVYWFIYLRT
                     GSQPR"
     gene            complement(4377..7100)
                     /gene="fdhF"
                     /locus_tag="PLS35_16120"
     CDS             complement(4377..7100)
                     /gene="fdhF"
                     /locus_tag="PLS35_16120"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNI20248.1"
                     /translation="MKLSINGRVIVASPGDSIYRAAKQAGIGIPGLCTSDHLNPFGSC
                     RLCLCEVEGQSGTPASCTTPVREGMVVHTESERLTRLRKHLLELYLSEQPAGDRVPEP
                     LQALARSLGLTHVRYLQPAGRVPVVDRSNPFFVFDNAVCISCARCVRACDEIQGTHAL
                     TMLYRGFSSRPAAGAAALSGEAAGFASSNCVSCGSCVKECPTGALIEKTVLEEGEPVQ
                     AVRTTCAYCGVGCTFDAGVREGRVVRMGPADDGPSNHGHACMKGRFGWTYNYAPDRLR
                     TPLLRQGDKWVELSWPAALDRIAEEWSRIKEAHGPDALATISSSRGTNEENYLFGKFM
                     RCVIGSNHIDNCARVCHSATVTGMMETLGASAATNSIHDLDLAKLILVVGANPTESHP
                     VLGARIKQAARRGVPLIVIDPRRTELARLADLHLQLHPGTNVALLNGMGRVIAKELLT
                     DAAFVRDRTEGFEEWLNVVEGCTPELTSRLTGVPAHLITEAARRYATSGGSMAVHGLG
                     MTEHRWGSHGVMALVNLALATGNIGKPGTGINPLRGQNNVQGASDVGCLPTFFAGYQP
                     LTDPALAAAHLAVTGRPLPTARGMKTPDMWDAALAGTLKSLWIIGYDVAQTDPNLKKV
                     HAALKQLDFLIVQDLFFSETAKLAHLVIPGASFLEKDGTFTNLERRIQRIRKAVEPPD
                     GVLPDWQVVCEVSARMGYPMQYSHPSAIMDEIAQLAPLFAGVSFDRLDAPEGLQWPVP
                     SKDHPGTSLMHEQSFPKGKAKFVAVDYLPPGETPSEAYPLVLITGRILQHYNCGAQTR
                     RTEIMQVVDTDVLEMHALDATRLDVRDGDPVRLISARGEARLPVMISDRVQPGELFTS
                     FHFPDSDLNVLLSSSADESSKCPEYKVSTVRVEKVLSTGTPTPSMHVMLIT"
BASE COUNT         1585 a         2268 c         2002 g         1331 t
ORIGIN      
        1 ggccagcatg tagcacattt catcgtggaa ggcggtcgca tcttccggcg ttttgaaata
       61 gccgtgattc ttgccccaga aggtccagca tcccgccaac cggttgaaca cctgacggga
      121 atcacgctcc cctcccaaga ccggtttgcc tgcggcgtcc accaccggcg tgccgtcctc
      181 gtgcacttgc ggcactccgg ccttccggaa atatttttgc gccagaatat cgatcgccaa
      241 ttgcgtccaa tgttccggga tatcgatatg ctccaacttg aacacggtcg atccgtctgg
      301 attccggatc tccgaggaac gtttcacgaa cgcaagaccc tcataggggc tctgcccacg
      361 cttggtgaat cttcgttcaa ttctcactgg ttcctcctta agagacgcct aacgttcggt
      421 gcaggtgccg gtcggcaagt atatgtggtt ggggatggga aagagtcctg tccatccaga
      481 ggtacatttc gcgtcgaaac actctctcga ctgcattgca tactagatat agtgatccca
      541 tttttttgtc aaacaaaatg ttgtggcaca cctgtgggta atggggataa gctggtgata
      601 gcgtctccgc taggtttcat gcagcttttc tccatttttt gctgagttat ccacaggcgt
      661 tcagagactg tggaggaagt ttttcacagg ggagcgagaa aaatgctggc acaaatcaaa
      721 atgcgagccg accgaccttc accgtcgcct cctcgacccc tcgtaacggg acaaattggc
      781 ctccgatgcc cagcaccagc accggttggt ccagcacatt cgtttcaaac agactgagca
      841 gcccccagtt gttccgtctc gtattgggcc ggacgactcc ttcgacgagc ttcggccctt
      901 gtgcgcggaa aatcgaacga atcagatcgc ggtcgcggcg cgggccggat tgatccatct
      961 tatcgatctc agcggacaag cgcgcttcga cgaactgcag ataggtgtcc atcgtcggat
     1021 tggtcgccgc cagccacacc gccagcatca aacttcccac caatccaacc agggtcccag
     1081 tacgcatcac acaccttcac cttgccgccg cacgatcgtt gcgcgccggg agcaaccacg
     1141 gtttgacaaa tgcacctcgt ctgtttagct tacgcccgat ttccaaggaa tggaatacga
     1201 aggcgcgtga tcgcgccggt tcgacagtca cggaggatgc actatgttcg tttggagtaa
     1261 acgcctcgta accggtctgc tggcaggttt cgtcctgctg atcggaatct tcctgcttca
     1321 gggagagccg gaaacaatca gcggcttgaa gatccacacc acccgctgga tggcggtgaa
     1381 ctatatcggc ctgctggtgt ggctgtttgt atggatgttc gaccaagccc gggtgcgcgg
     1441 gaaaaacgtg ttcttctggc tggtgccgtt cgcattggca ccgctgccga ccctgatgct
     1501 gtttattttg tttctgcaga ggaagatgaa ataggaacag acggcacacc tgcgggaaga
     1561 gttttgctca ggaactaaca gggcgctccg gccgcggacc tccccgcatt cagttccgca
     1621 tgttgggcaa ccggcttacg aagcacccac agcagccaca gacccggcag cgcggcgagg
     1681 aaggtcacaa agaaaaacgg accccacccg gccagcccca ccagtttcgc cgcaggccaa
     1741 ccgaggaaca cgcgaccgag cgcttcaacg gatgacagca gcgcgaactg ggtggcggta
     1801 taccgatgat tgcagagcga catgaccagc gccacgaagg cggcagtgcc catgcctcca
     1861 gtcagatttt cggctcccac cgcaaacgcc atcacggcat agcttttccc cgcccaagcc
     1921 aaccacataa atgacaggtt cgagagggcc tgcaggacac cgaacacaaa cagcgaacgg
     1981 aacagcccta aacgcggcat caacccgccg ccgataaacg cgcctagaat cgtggctccc
     2041 atcccgaatg cgcggaccac cccgatatcc tcggaagaaa actgcatccc gcgcagcaaa
     2101 aacgacgtcg tcagcgcgcc ggcgaacgca tcgccaagtt tatagagcac gatcaaaccg
     2161 agcagcgcta acgccatagg acgggaaagg aactcgctca gcggccccca gaccgcttcc
     2221 gccatcgatt tgggcgccac ctgcgcatcc tctggttccg gactcaggag gatcgtcacg
     2281 atacccaatg ccatcaaggc ggacatacag agatacgccg ccgaccagtt caagcgggca
     2341 gccaggatca gggccaacga accggatgca atcatggcaa tccggtagcc catcacccag
     2401 gtcgccgcgc ccaatccccg ttcctcgggt ttcagcaggt cggtccggta cgcatcgaac
     2461 acgatatcct gcgaggcgga aagaaacgcc acggcaagag ccaaggccgc aaacacctgc
     2521 gcgccctcag ccgggccgat caaggccatc acagtcaggg caagcgcgag accgctttgg
     2581 gtgacgagca tccacccgcg acggcggccg agccagggcg gcacgatccg gtccatcacc
     2641 ggcgcccaga gaaatttcaa ggtataggga aggccaacat aggcgaacag gccgatcgtc
     2701 gtgagatcca gcccggcctc cgtcagccat gcctgcaacg ttccgcccgt gagggccagg
     2761 ggcagacccg aagcaaaccc cagcggcagc atgatgccga ggcgtggggt cagatagctg
     2821 cgaatggatg aatggctcat aggatcaaga gaacagcagg ctcacgaggt gcagcatacc
     2881 attgcccgag acgctgacgg catcagaatc gtgattcttt ctgtaaggat ccggaacgat
     2941 actgtttaca ggccgccgga aactcgagcg ctgtacgatc tcttcccgcc aaacttctcc
     3001 ggtatgaatc agaggaggct tttgtatagc tgaactggta taagtagcaa aacgagaggg
     3061 atgagatgaa cgtgacaaac ttaggcttca cgtggaattc ccgatacctc gaactgtcgc
     3121 cgacggagat ggcgatcgcc cttgtggcgg cccttctggt cggcggagcg gccctgtatt
     3181 ttctcctcaa agtcctgcgc agcaagccgg tcaaggtgct cggcatggca ttgttggtgg
     3241 ccggaatcgc actggtcggc tggctggctc tcgatcagca tcaacaggat gagcgcgcgc
     3301 gcgtctccga aatctggagt cgcaccttcg aaaacagcgc gatcaaagac ttcgaatcct
     3361 gcgtcgccaa ccggacacct cgcagcccag ggccgcaata ccgcgtggag gtcgtgaccg
     3421 aatgcggtga cgtggcgaaa gccattaaag aagggttgga ggcccgctga ccggtatcct
     3481 agccgcccga actctacctg ccgtcccctg ccaacataaa ccacaggatg ctcttcatcg
     3541 tggctgggaa ccggtcctga ggtagatgaa ccaatagact agcgcgacta acactgttcc
     3601 gccgatgatg ttcccgaggg tgaccagcgc caggttgctc agggcgtcta tgagcgacag
     3661 gggcgccgca ctgcccgccg ccagcgctat ccccagcggc aaaaagaaca tgttcgccac
     3721 ggaatgttcg aagccgcaag cgacgaaggc gctgatggga aacacaatcg cgagaatctt
     3781 atccgccacg ctccgcgcac ccatacacaa ccagaccgcg agacacacca gtacattgca
     3841 caaaatgcca cgcgcaaagg cagacagcgg atcgagggcg attttactgc gcgcgatacg
     3901 caccatcgcc tcgccgacag ccccgtcccc tagcaactga atccccgcaa gcaacaccag
     3961 tcccgccgtt ccgcaggctc ccagcaagtt tccgagatag acccagatcc aattcctcac
     4021 gacctgacgc gtctgcacac aaccggcagc ccaggccatc gcaatcagat tgtttccggt
     4081 aaacagttcc gccccgccaa ccacgaccaa aatcaggccc aggctgaacg tgacgccccc
     4141 ggtcagccgc aagagtccga atggcaacgg cgaaccgtct tgtcccgccg tgatcgtgac
     4201 ggtgtaaaac aaggcgccca acgaaatgaa cgcgccggcc agcacggcca gggctaccat
     4261 cgtcggcacc gccgtgttca ccttggccag cccgagcttg cacacacgcg cggcgatttc
     4321 agggggcgga taggcgtcgg cgaccgagcc ggtggcggaa ggaactggct cggcagtcat
     4381 gtaatcaaca tcacatgcat ggagggagtg ggcgtcccgg tcgacagcac tttttcaacc
     4441 cgcaccgtcg agactttgta ctcaggacat ttcgaacttt cgtcggcgct ggatgacagg
     4501 agcacgttca ggtccgaatc ggggaaatgg aaactcgtga acaactctcc cggctgcacg
     4561 cggtcactga tcatcacagg cagcctggcc tccccgcgcg cgctgatcag cctgaccgga
     4621 tcgccgtcac ggacgtccaa cctggtcgca tcgagcgcat gcatctccag cacatcggta
     4681 tcgaccacct gcatgatctc cgttcgcctc gtctgcgccc cgcaattgta atgctgcaga
     4741 atcctcccgg tgatcaatac cagcggatag gcttcgctcg gtgtctcccc cggcggcagg
     4801 taatccactg ccacaaactt cgccttgcct ttgggaaacg actgttcatg catcaatgat
     4861 gtgccgggat ggtccttcga gggcacgggc cactgtaatc cctccggcgc atccagtcga
     4921 tcgaacgaca ccccggcaaa cagcggcgcg agttgcgcta tctcatccat gatggccgag
     4981 ggatgggaat actgcatcgg ataccccatc cgcgcggaga cttcgcagac gacctgccag
     5041 tccggcaaca cgccgtctgg cggctccaca gcttttctga tgcgttggat gcggcgctcg
     5101 agattcgtga aggttccatc cttttccaga aatgaggccc ccggaatcac gagatgcgcc
     5161 agcttggccg tttcgctgaa aaacaaatcc tggacgatga gaaaatcgag ttgcttgagc
     5221 gccgcatgaa cttttttcag attcgggtcg gtctgagcca cgtcatatcc gataatccag
     5281 agactcttga gcgttcccgc cagcgcggca tcccacatgt ccggcgtctt catgccgcgg
     5341 gccgttggca agggccgtcc tgtgactgcg agatgcgccg ccgccagcgc tggatccgta
     5401 agcggctgat acccggcaaa gaatgtgggc aggcagccga catccgatgc cccctgcaca
     5461 ttgttctgcc cgcgcaaggg gttgatcccg gtaccgggct tcccgatgtt gccggtcgcc
     5521 agcgcgagat tcaccagcgc catcacccca tggcttcccc agcgatgttc ggtcatgccc
     5581 aggccatgca ccgccatgga gccgccgctt gtggcatatc gtctcgcggc ttcggtgatg
     5641 agatgagcgg gcacgccggt cagcctcgac gtcagttcag gagtacaacc ctccaccaca
     5701 ttcagccatt cctcgaaccc ctcggtccgg tcacgcacga atgccgcatc ggtcaaaagt
     5761 tccttcgcga tgacacggcc catgccattc agcagcgcca cgttggtacc aggatgcagt
     5821 tgaagatgaa gatcggccag ccgggctaat tccgtgcgtc gcggatcaat cacgatcaac
     5881 ggcaccccgc gccgggctgc ttgtttgatg cgcgcgccga gtaccggatg ggattcggtg
     5941 ggattagctc cgacgacgag gatcagcttg gccagatcaa gatcgtgaat ggaattggtt
     6001 gccgccgacg cgcccagagt ttccatcatg ccggtcacgg tcgcgctgtg gcacacccgt
     6061 gcgcagttgt cgatatgatt gctgccgatc acgcagcgca tgaatttgcc gaagagataa
     6121 ttctcttcgt tcgtgccgcg gctggaggag atcgtcgcca gcgcatcagg cccgtgggct
     6181 tccttgatgc gactccactc ctcagcaatt cgatcgaggg ccgcaggcca ggagagttca
     6241 acccacttgt cgccctgccg cagaagcggc gtgcgcagcc ggtccggcgc ataattgtag
     6301 gtccacccga atcgtccctt catgcaggca tggccgtgat tggaaggccc gtcatcggcc
     6361 ggtcccatcc ggacgacccg cccctcccgc acaccagcat cgaatgtgca accgacaccg
     6421 caataggcac aggtggtgcg aacggcttgc accggctcgc cctcttcaag cacggtcttc
     6481 tcgatcaagg cccccgtcgg acattccttc acgcaagacc cgcaggagac gcaattcgat
     6541 gaggcaaacc ctgccgcctc tccgctcaac gccgcagccc ctgccgccgg ccggctggag
     6601 aatccccgat acaacatcgt cagggcatgc gtgccttgga tctcatcgca ggctcgcaca
     6661 cagcgggcgc atgaaataca caccgcattg tcgaagacaa aaaagggatt ggatcgatcg
     6721 acgacaggca ctctgccggc aggctgaaga taacgaacat gcgtcaaccc caacgatctg
     6781 gccaacgcct gcaagggttc cggcacgcga tctcctgccg gttgctcgga gagatagagc
     6841 tcgaggagat gttttctcag acgagtgagc cgttcgctct ccgtgtgcac gaccatgcct
     6901 tcgcgcaccg gcgtggtgca ggaagccggc gtcccgctct ggccctcgac ttcacacaga
     6961 cagaggcgac aggagccgaa cggattcaaa tggtctgagg tacagagccc ggggatgccg
     7021 attcccgcct gtttcgccgc tcgataaatg gaatcacccg gcgacgccac gatcactcgt
     7081 ccgttgatgg aaagtttcat aatgatgcgc gctcagcctc agctttcagt cacgactgac
     7141 gtctgaccgc tgacagctta tttctaaatt ccacgggaaa atgttc
//
LOCUS       DAOQEW010000191         7174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3764_length_7174_cov_9.147071, whole genome shotgun sequence.
ACCESSION   DAOQEW010000191 DAOQEW010000000
VERSION     DAOQEW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_7174_cov_9.147071"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..274)
                     /locus_tag="PLS35_16125"
     CDS             complement(5..274)
                     /locus_tag="PLS35_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20249.1"
                     /translation="MKGLRFERLGRDRHYNIIFHIGTSYVPVSDETIDELKAQSLLSS
                     ERFLDLLIDKIGYTNYLKEQIRGELQASGDPMTQMTVLQGAIREL"
     gene            complement(390..731)
                     /locus_tag="PLS35_16130"
     CDS             complement(390..731)
                     /locus_tag="PLS35_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HNI20250.1"
                     /translation="MAALTLHPMKEIRVIVSGEHRPYVTELLDKVEATGYTIIGNISG
                     KGHHGVREAHFMFSEQESLVMIMAVVPEEKVEPVLAGLRPLFDRHSGVMFVSDVSVSR
                     REYFGKKSGKP"
     gene            complement(777..4046)
                     /locus_tag="PLS35_16135"
     CDS             complement(777..4046)
                     /locus_tag="PLS35_16135"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HNI20251.1"
                     /translation="MIRTDEPADLEPRRMELRGMIRLASEVIAQYWPMRTFVHHNPLH
                     SLEYLPFTETVRRGQQFLGGHGYLPGDLYRRYVKSGRILIRHIDDALAALAKDEEVDL
                     GSCRISQREVMRACLTHGLSRPAEEPLDRLMECEPNEEQVDVLAERLVALSVPTVQDQ
                     MAAAVREDVAALGRDLTLSNWCDRTLGTRIVDQINGELIKWCEAFLDEGHATWSMPGR
                     RQGLYAAWKQVAANEWATCGIADSRRKIAALPEHPEDAVLDCLEALAIPAEFRQDYLG
                     LQLAALPGWAGFIKWRAEENDYAWQQAYPVGLVKFLAVRLWYVRELVEKVCQEELGIE
                     GNYRAVLRFMDQESAGYFMRKEWVAGRLPAAYADRVARLHAQSGSQHNRAALSREWEQ
                     LTHHYQIEFGPRRERAAQRAMARRLLTLAQALDIVPTLLMDAHPTALRQLLDWMAAFP
                     ESAHGPVWLKAFEAGYQDHLFGLLRRAPTRPQPAAPDGQPPVRPQSQSVFCIDVRSEP
                     FRRHLESTGANETYGFAGFFAALIRYRAWGKEHDTEQFPVIMRAKNEVREIPRSYLDH
                     YVSKHQSRAKLVHAGHTLLHDLKENVVTPYVMVESLGWFYVLPMMGKTFLPALYKRLT
                     NWIRRLFVPPIATILTVDKLAPAETEEMVVSEQRALIWKALRDRLGLHGSRVEAEFVE
                     ALRRRALDEDAPVEPFLSEAARAVDLSNEQLTTFLDELRRHYRINRRAASRQKERITR
                     TGFTLDEQVLTLETALRMMGLVRNFARLVLFCAHGSTTENNPFESALDCGACGGNEGK
                     PNARVLAAMANRAPVRERLAKRGIIIPADTHFLAGQVDTTTDEVQLFDLEDAPPTHRK
                     DVARLYDDLREAAQLTSQERCARFPDVGAVLPLDKAAGHVAARSADWSQVRPEWGLSG
                     NTAFIIGRRELTKGLNLSGRIFLQSYDYREDPTDRWLEVLLTAPQVVAQWINMEHYFS
                     AVDNDVYGSGSKIYHNVVGRIGIMSGPWSDLRLGLAWQTVMNDELPYHEPMRLMTLVE
                     ASRARIEKLIARHEILQHFYHNEWVHLAALDPEDGTWYRYMPSGVWRRVQHPSETTQV
                     G"
     gene            complement(4043..5335)
                     /locus_tag="PLS35_16140"
     CDS             complement(4043..5335)
                     /locus_tag="PLS35_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20252.1"
                     /translation="MTPWLLLAVPLLGASLALLMRAALQRMKTVALTTTGLTLLAVIG
                     TSLAQNEATAAMSLLCLLPATAFLTLLGQPLHRDNSSAWVMTLILLGVALGILTSHAP
                     THELLLILLLGLVCGLLYRHQPGTAPAAWRGMASFGLGILTALLALALPAPAATIASL
                     VTCATLLPLLPLHSGFVAALSGLPGNLPALLAFILPTVGFHSLYLLLPSLSGTVLQTV
                     ALLALAGAVYGSLRALIQSHPLPRLGYASSAFFCMLWWYVADTGTAPVQALVYLSAVG
                     LTTSGLLLAWYAIRARYGDVDFRALGGLAYPMPRFSTLLALLGLAALGMPPFGVFSGF
                     LGMLLTPAFAPSGPFAIIMLVWLTASWYYTDLIQQVVFGRPRLDIRHEDLRQTEWISL
                     MLVLLLLLILGMAPSRMFESHTTAPSSTVAIKGETWTR"
     gene            complement(5409..7103)
                     /locus_tag="PLS35_16145"
     CDS             complement(5409..7103)
                     /locus_tag="PLS35_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNI20253.1"
                     /translation="MSFLALVPLLPLLTAFIVMTGDRADQEQNARSGLFPIAASFLGS
                     LIILIVVTSDGPIMFQLYDPAAIANLAFPIGVYIDRLSAVMMVLITGVTTLIYRYSMG
                     YMYQDRGYRRYLGMLGITTSVLLCMVSSANLVMLFIFWQILSWLLFLLAHNHGHAATL
                     SGASNTFTTLRLSDAAFLAGIVLAYSLYGTFEFQTLFTRAAETPVTLSIWPELGWTMD
                     GVTAVTLLIFGGAMGKSAQFPIHTWLPRSLYAPTPIHALLHAGIINAGGFLLNRLAPL
                     YGLSPTTLHVVFVIGMLTAILGATMMLTQNDIKKTLGFSTIGQMGYMIMECGLGAFSL
                     AVFHLIAHGLFKGTVFLNCGNVIHKARQEPSFPPIDRETEESEFSNLTWSTGFFTTLL
                     LPLVILLVTHGVLRIPLLESQGTVIFLFFIWVTSSQAILSLTRIRAVASWKVSAAMLV
                     TLVIVVFTYLFAVESFTHFLYPNPEEVAFYFKAAALPGRLFDSLVMGTTLLTILSWVY
                     LYAHAHGRTITIPGWIEAFLVRLYVLFMNRLYLDQLYLNIGRVVTQVAHRLEKRLS"
BASE COUNT         1519 a         2116 c         2251 g         1288 t
ORIGIN      
        1 ggatctacag ttcgcgaatg gcgccttgta ggacggtcat ctgagtcatg gggtcgccgg
       61 aggcctggag ttccccccgg atctgttctt tgagatagtt ggtgtagccg attttatcga
      121 tgaggaggtc gagaaaacgt tcggaggaca acagactttg cgccttcagt tcgtcgatgg
      181 tctcgtcgct caccggcaca tagctggtgc cgatgtggaa aatgatgttg tagtggcgat
      241 cgcgcccgag tcgttcgaac cgtaatccct tcacgtgccg tccttcctct tacaggccaa
      301 cgggggcaca ttgtagacaa tcactcatga gaagacaagg tcagtctggg gccctgcggc
      361 cccgccagcc gtggcttccc ggctaccggt cagggcttcc cgctcttctt tccgaaatat
      421 tcccgccggc tgacggacac atcagacacg aacatgactc ctgagtgccg atcgaacagc
      481 ggccgcaatc cggccaagac cggttccacc ttttcttccg gcaccaccgc catgatcatg
      541 accaggcttt cctgctcact gaacatgaag tgcgcctcgc gtacgccatg gtgtccctta
      601 ccggagatgt tcccgatgat cgtgtacccg gtcgcttcga ccttgtccag caattccgtg
      661 acgtacggcc gatgttcccc ggacacaatg acacgaattt ctttcatggg atgcaatgtg
      721 agtgcggcca tgtctcgact cctttgaaaa tgatcgtcgg ttgcgacagt gagcggttac
      781 cctacctgtg tggtctcgct cggatgctga acccttcgcc atacgcccga cggcatgtag
      841 cggtaccagg tgccgtcttc aggatccaac gcggccaggt ggacccactc attgtgatag
      901 aaatgctgca ggatctcatg ccgcgcaatg agtttttcga tcctcgcgcg cgaggcttcc
      961 acgagcgtca tcaggcgcat tggctcatga taaggcagct catcgttcat gacggtctgc
     1021 caggccaggc cgagacggag atcgctccag gggcctgaca tgattccgat gcggccgacg
     1081 acgttatgat aaatcttgct cccgctgcca tacacgtcgt tgtctacggc tgagaaataa
     1141 tgttccatgt tgatccactg cgccaccact tgcggggcgg tgaggagtac ttcgagccag
     1201 cgatcggtcg ggtcctcgcg ataatcgtag gattgcagaa agatgcgccc ggacaggttc
     1261 agccccttcg tcagttcccg ccggccaatg atgaaggcgg tgttcccgga caggccccac
     1321 tcggggcgca cctgactcca gtccgcgctt cgtgccgcca catgacctgc tgccttgtcc
     1381 aagggcagga cagccccgac gtcgggaaat ctggcacagc gctcctggct ggtaagctgg
     1441 gctgcttccc gcagatcgtc atacaggcgg gcgacatcct tgcggtgggt cgggggcgcg
     1501 tcttcgagat cgaacagctg tacctcatcg gtcgtggtat cgacctgccc cgcaaggaaa
     1561 tgcgtatccg caggaataat gatgccgcgt ttcgccagcc gttcgcgcac cggagcgcgg
     1621 ttggccatcg cggccagcac acgggcattc ggtttgccct cgttcccgcc gcaggcgccg
     1681 cagtccagcg ccgactcgaa cggattgttt tccgtcgtgc tgccgtgggc acagaagagt
     1741 accagccggg cgaaatttcg taccaggccc atcatgcgca aggccgtttc gagggtcagg
     1801 acctgctcgt cgagtgtgaa accggtgcgg gtgatgcgtt ctttctggcg cgaggccgca
     1861 cgtcgattaa tgcgatagtg ccgccgcagt tcatcaagga aggtggtcag ttgttcgttg
     1921 gacagatcga ctgcgcgggc ggcctcactg aggaatggtt ccactggcgc atcctcgtcc
     1981 agggcccgac gtcgcaacgc ctcgacaaat tccgcctcga ctcgcgaacc gtgcaggccc
     2041 agccggtcac gcagggcttt ccagatcagg gcgcgttgct cggagaccac catttcttcc
     2101 gtttccgccg gcgccagctt gtcgacggtt aaaatggtgg cgatcggcgg cacgaacaac
     2161 cgccggatcc agttggtcag gcgtttgtac agcgccggca ggaaggtctt gcccatcatg
     2221 ggcaggacgt aaaaccagcc gagcgattcg accatcacat agggcgtgac gacgttttcc
     2281 ttcaagtcgt gcaggagcgt gtgaccggca tggaccagct tcgcccgcga ctggtgtttg
     2341 gacacgtaat ggtccaaata gctgcgcggg atttcgcgga cttcgttctt tgcgcgcatg
     2401 atcacgggaa actgctccgt atcatgctcc ttgccccacg cgcgatatcg gatgagggcg
     2461 gcgaaaaatc ctgcgaaacc gtaggtctca ttggcgcctg tggactccag atgacggcgg
     2521 aacggttcgg atcgcacatc gatgcaaaag accgattggg actgggggcg gaccggcggt
     2581 tgaccgtcgg gggcagccgg ttgcggcctg gttggcgcac gccggagcag gccgaacaga
     2641 tgatcctgat agcctgcctc aaacgccttc agccaaaccg ggccatgggc cgattccgga
     2701 aaggcagcca tccaatccaa gagctggcga agcgcggttg gatgggcgtc catcaagagg
     2761 gtcggcacaa tgtcgagtgc ctgcgcgagc gtgagcagac gacgcgccat agcccgctgc
     2821 gcagcccgtt cgcgacgcgg gccgaattcg atctgataat gatgcgtgag ttgttcccat
     2881 tcacgggaga gagccgccct gttgtgttga ctccccgatt gcgcatgcag gcgtgccacc
     2941 cgatcggcat aggcggccgg caggcggccg gcgacccatt ccttgcgcat gaagtatccg
     3001 gccgactcct ggtccataaa ccgcagcacc gcgcgatagt ttccctcgat gcctaattcc
     3061 tcctgacaga ctttctcgac gagttcgcgc acataccaga gacgcacggc gagaaatttc
     3121 accagaccga cagggtaggc ctgttgccag gcatagtcgt tttcttcggc ccgccatttg
     3181 atgaagccgg cccaaccggg cagcgcggcg agctgaaggc caaggtagtc ctgccggaat
     3241 tctgccggaa tcgccaacgc ctcgaggcaa tccaagaccg cgtcttccgg atgttcgggc
     3301 agcgcggcaa ttttgcgacg gctatcggca atgccgcagg tcgcccactc attagcggcc
     3361 acctgtttcc acgctgcata cagcccctga cgccgtcccg gcatcgacca ggtggcatgg
     3421 ccttcgtcga ggaaggcttc gcaccatttg atcaattcgc cgttgatctg atccacgatc
     3481 cgggtgccca gagtccggtc gcaccagttc gataaggtaa ggtcccgtcc cagcgcggcc
     3541 acatcttcgc gcaccgcggc ggccatctgg tcttgcacgg tcggcacgga caaggcgacg
     3601 aggcgttcgg ccaagacgtc cacctgttcc tcgttcggtt cgcattccat gagccggtcg
     3661 agaggttcct cggccgggcg agacagtcca tgagtcaggc aggcacgcat gacttcccgc
     3721 tgtgaaatac ggcaggaccc gaggtcgact tcttcatcct tcgccaaagc ggccaacgcg
     3781 tcgtcgatgt ggcgaatcag gatccgccct gatttgacgt atcgacggta gaggtcgccg
     3841 ggcaggtagc cgtggccgcc aaggaactgc tgcccgcggc ggaccgtttc agtaaacggt
     3901 agatactcga ggctatgcag cggattatgg tgcacgaagg ttcgcatggg ccagtactgc
     3961 gcgatgactt cgctggcaag ccggatcatg ccgcgcaatt ccatgcggcg cggttcgaga
     4021 tcggcgggct cgtcggtgcg aatcatcggg tccacgtttc ccccttgatg gcaaccgtgg
     4081 aggagggagc agtcgtgtga gactcgaaca tccgggatgg cgccatcccc aggatcagca
     4141 gtaagagcaa caccagcatc agggagatcc attccgtctg tcgcaaatct tcgtgccgga
     4201 tgtccaggcg tggccgaccg aacaccacct gttggatgag atcagtgtag taccacgagg
     4261 ccgtgagcca gacgagcatg atgatggcga acggcccgga cggcgcaaac gcgggggtga
     4321 gcagcatgcc caggaagccg gaaaacacgc cgaacggcgg catgccgagg gcggcaagcc
     4381 cgagcagcgc caggagagtg ctgaaacgag gcatgggata ggcgagcccg cccagggccc
     4441 ggaaatcgac atcaccgtac cgagcccgga tggcgtacca cgccagcagg agtccgctgg
     4501 tggtgagtcc cacggcgctc agatagacga gggcctggac cggcgcggtt cccgtatcgg
     4561 ccacatacca ccacagcatg cagaagaatg ccgaagaggc atagcccaat cgcggcaggg
     4621 gatgcgactg aatcaaggcc cgcagcgagc catacacggc gccggccaac gccaggaggg
     4681 ccaccgtctg gagtactgtc ccggagagtg acggcagcaa caggtacagg ctatggaatc
     4741 caacggtcgg cagaataaac gccaacaacg cgggaagatt gcccggcagt ccgctgagtg
     4801 cagccacgaa tccgctgtgc aggggcagca ggggcagcag cgtggcgcag gtgaccaacg
     4861 aggcgatcgt tgcggctggt gctggaagcg ccagcgccag caaggccgtg aggatgccca
     4921 gaccgaatga agccatccct cgccaggctg ccggcgcagt gcccggttga tgacgataga
     4981 gcaagccgca gacgagaccg aggagcagga tgagcaagag ctcgtgggtg ggcgcgtgac
     5041 tcgtgagaat gcctagtgcc acaccgagca ggatcagagt catcacccag gctgaactgt
     5101 tatcccggtg caagggttgc cccagcagcg tcagaaacgc cgttgccggg agcaggcaca
     5161 acaacgacat ggcggcagtc gcttcgttct gtgccaggga ggtgccgatg acggcaagca
     5221 gagtcagccc ggtcgtcgtc agggccaccg tcttcatgcg ctgaagggcg gctctcatca
     5281 gcagggcgag gcttgcgcct aacagcggaa cggccaggag tagccagggt gtcatccagc
     5341 tctcgatcac ggctggactc cgatcacgga cgcagcagcg cggcgagaca tgcgcagtct
     5401 gaatgcagtc acgatagccg tttctccaga cgatgcgcga cctgtgtgac gacgcggccg
     5461 atgttgaggt acagctgatc cagatacaac cggttcatga acaggacata caggcgcacg
     5521 aggaacgctt cgatccatcc ggggatggtg atggtgcgtc cgtgagcatg ggcatacaga
     5581 tagacccagc tcagaatcgt caacagcgtc gtgcccatca ccaggctgtc gaaaagccgt
     5641 ccgggcagcg cggcggcctt gaaatagaag gccacctctt ccggattcgg gtacagaaaa
     5701 tgggtgaagc tctcgaccgc aaacagatag gtgaagacga cgataaccag cgtgaccagc
     5761 atggcggccg ataccttcca ggaggccacg gcgcgaatgc gcgtcagcga gaggatcgcc
     5821 tgcgaagacg tgacccagat gaagaacagg aagatcaccg tcccttgtga ttcgagcaac
     5881 ggaattctca acacgccgtg agtgaccaac aaaatgacca ggggcagcaa cagcgtcgtg
     5941 aagaaccccg tcgaccaggt caggttggag aattcgcttt cctccgtctc ccggtcgatc
     6001 ggcgggaacg agggttcctg gcgggcctta tggatgacgt tgccgcagtt caggaacact
     6061 gtccctttga atagcccgtg cgcgatcaga tggaagacgg caagggaaaa ggctccgagg
     6121 ccgcattcca tgatcatgta gcccatctgc ccgatggtgg aaaatcctag ggtcttcttg
     6181 atgtcattct gtgtcaacat catggtggcg ccgagtatcg cggtgagcat gccgatcacg
     6241 aagacgacat gcagcgtcgt cgggctcagg ccatacagcg gggccagccg gttcagcagg
     6301 aatcctccgg cgttgatgat gccggcatgg agcagcgcgt ggatcggagt cggcgcgtac
     6361 agggaccgcg gcagccacgt atggatggga aattgggccg attttcccat ggcgccgccg
     6421 aagatgagca gggtcaccgc ggtgacgccg tccatggtcc agccgagctc aggccagatc
     6481 gacaaggtca ccggcgtctc ggcagcgcgt gtgaacagcg tctgaaattc gaacgtgccg
     6541 tacagcgagt aggccaggac aatgcccgcg agaaaagccg catcgctcag tcgtaacgtc
     6601 gtaaacgtat tggacgcgcc ggacaatgtc gccgcatgcc cgtgattgtg cgccagcaga
     6661 aataacagcc aggagagaat ctgccagaag atgaagagca tcaccagatt agcgctggaa
     6721 accatgcaca gcaggacgct ggtggtgatg ccgagcatac ccagataccg gcggtacccg
     6781 cgatcctggt acatgtaccc catcgaatag cgatagatca gcgtggtgac accggtgatc
     6841 aggaccatca tgacggcgct gagccggtcg atgtagacgc cgatggggaa cgccagattg
     6901 gcgatggcgg ccggatcata caattggaac atgatcgggc catccgacgt cacgacgatc
     6961 agaatgatga gcgagcccag gaaagaggcg gcgatgggga acaggccgga tcgggcattc
     7021 tgctcctggt ccgcacgatc gccggtcatc acgatgaagg ccgtgagtaa cggcaacagc
     7081 ggaacgaggg ctaagaagga catgcgaatc ccctgctggc catgaaaaga aaaaggccag
     7141 ccgacggtcc ttcacacaag gaaccaacga ctgg
//
LOCUS       DAOQEW010000192         6977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_3927_length_6977_cov_9.069633, whole genome shotgun sequence.
ACCESSION   DAOQEW010000192 DAOQEW010000000
VERSION     DAOQEW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_6977_cov_9.069633"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLS35_16150"
     CDS             complement(<1..767)
                     /locus_tag="PLS35_16150"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNI20254.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNVEEKLMIAKQLARLGVDIIE
                     AGFAYSSPGDFEAVRRIALEVEGPVVCSLARARPEDITRASEALKGAPRVRIHTFLST
                     SDIHLKHQFRMTREEAKKRAVEMVQLARTYVDDVEFSPMDASRSDPAYLCEVIEAVIA
                     AGAGTINIPDTVGYAVPQEFGGLIKRIKDTVPNSSQAIISVHCHNDLGMAVANSLAAV
                     VAGAGQVECTINGIGERAGNTSLEEIVMGLRTRRDWYG"
     misc_binding    complement(938..1182)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1179..1982)
                     /gene="pssA"
                     /locus_tag="PLS35_16155"
     CDS             complement(1179..1982)
                     /gene="pssA"
                     /locus_tag="PLS35_16155"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNI20255.1"
                     /translation="MKSPTMRPPFAKGHRKRQAMYLIPNLCTTGNLFCGVFAILSVFN
                     GQHLVAAIAILVGMIFDMLDGKLARLTNSTGQFGIEYDSLSDVVSFGVAPGVLIYSYA
                     LSGQGMFGVAVMFAYVAMGAVRLARFNATVSTSDSKYFTGLAIPAAAGVIASLVIFDL
                     HITQLGSEVKPLLILVITFGLAFLMVSTIKYRSFKDMKFRRGDHFTYLVWGILALMLI
                     VAWPQAMLFVTFTGYAVSGPIARLWTMIAKSAGKPVAKTDGPVVETRES"
     gene            complement(1979..2629)
                     /locus_tag="PLS35_16160"
     CDS             complement(1979..2629)
                     /locus_tag="PLS35_16160"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNI20256.1"
                     /translation="MADRAVGIPIVKEGLPFVGGLAGATLLFGLAGWTIPTVVAAGFL
                     LFTAWFFRNPSRTIPQQPNVVVSSGDGKVIAIEEEFEPRYLKEKSIRVTVFLNVFDVH
                     VNRMPCAGTVEGVNYQPGQFLVASKPEATLHNEQNAVMLKTESGAKVLCVQVAGLIAR
                     RIVCWVGQGDRVQRGERFGLIRFGSRMDTFLPLGSKICVALGDRVKGGETIVGELR"
     gene            complement(2679..3692)
                     /gene="ilvC"
                     /locus_tag="PLS35_16165"
     CDS             complement(2679..3692)
                     /gene="ilvC"
                     /locus_tag="PLS35_16165"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNI20257.1"
                     /translation="MKIYYDKDADLQLIRGKKVAVIGYGSQGHAHSLNLKESGATVVV
                     GLREGSSWKKAEASGLKVMPVADAVKASDVIMILAPDEAQAAIYRQDIAPNLKPGSYL
                     AFGHGFNIHFGQIVPPADVNVFMVAPKGPGHLVRSEYTKGSGVPCLLAVHQDPSGNTR
                     QVGLAYASAVGGGRAGIIETNFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGYA
                     PEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDITRGPRVVTEQTKQEMKKI
                     LGEIQSGQFAKEWVLENQANRPVYNALLKKGEGHPIEEVGGRLRSMMPWLKKDQLVDK
                     NKN"
     gene            complement(3759..4277)
                     /gene="ilvN"
                     /locus_tag="PLS35_16170"
     CDS             complement(3759..4277)
                     /gene="ilvN"
                     /locus_tag="PLS35_16170"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNI20258.1"
                     /translation="MEHIISVTVENKFGVLSRVAGLFSGRGFNIESLSVAPTLDPSMS
                     QMTIVTSGDERIVEQIVKQLNKLIDVIKVVDLNESEFVSRETALIKVHTKAEDRAEAL
                     RIADIFRANVIDSTPSTYTIEVTGDPKKLEAIINLLQPLGIKELIRTGRVAIAREAIR
                     PALSQPKKLARE"
     gene            complement(4320..6098)
                     /gene="ilvB"
                     /locus_tag="PLS35_16175"
     CDS             complement(4320..6098)
                     /gene="ilvB"
                     /locus_tag="PLS35_16175"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNI20259.1"
                     /translation="MKLTGSEILIECLKAEGVKTIFALPGGVVLKIFDMLHQQKDIEV
                     VLTRHEQGAGHMAEGYAKATGKAGVCLVTSGPGMTNVITALADAYMDSVPLVCISGQV
                     STSLIGNDAFQEADNIGLSRPCTKYNFLVKDVNDLATTIKEAFYIATTGRPGPVLVDI
                     PKDVSMAKADFTYPSSVSIRGYNPTYEGNKWQIKQAAEAIMKAKKPILYVGGGVVFSQ
                     ASKELLELAEMTQIPVDMTLMGLGAFPGEHPQSMGMLGMHGTYCANMAVHYSDLVIAV
                     GARFDDRVTGKVSEFCPFAKVIHIDIDPTSIRKNIHVDIPIVGDCKAVLRELNQILRA
                     TVNGNQKDLRKPWWDQIREWQQAHPLAYQQEPDGAIKPQHVVKRLYELTKDRDPIVAT
                     DVGQHQMWTAQYFKLARPNRWLTSGGLGTMGFGFPAAMGAQAAFRDRLVLCVAGDGSI
                     QMNMQEMATAVVSKLPVKIIILNNRFHGMVRQWQDLFYEGRYASSYLDTTPDFVKLAE
                     AYGAVGLRAGKVGDLDAVLKEAISTDRPVVVDVPTYPYENCYPMIPAGGCNHEMILAD
                     PPELKQRMAGVPSTGSDENKDTILTA"
     gene            complement(6591..>6977)
                     /gene="rnpB"
                     /locus_tag="PLS35_16180"
     ncRNA           complement(6591..>6977)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS35_16180"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1485 a         2159 c         1803 g         1530 t
ORIGIN      
        1 ccgtaccaat ccctgcgtgt ccggagcccc atcacaattt cttccagcga ggtgttgccg
       61 gctcgttcgc cgataccgtt gatcgtgcac tcaacctgac cggcccccgc cactactgcg
      121 gcgagactat tggccaccgc cattcccaaa tcattgtgac aatgcacgga aatgatcgcc
      181 tggctgctgt tcggcaccgt gtccttgatg cgtttgatca ggccgccaaa ctcttgtgga
      241 acggcatacc ccaccgtgtc gggaatatta atcgttccgg caccggccgc gatgaccgcc
      301 tcaatcactt cacagagata cgctgggtca gaccggctgg cgtccatggg agaaaactcc
      361 acatcatcga cgtaggtgcg tgccaattgc accatctcca cggctcgttt cttcgcttct
      421 tcacgcgtca tccggaactg gtgcttcaaa tggatatccg acgtggagag aaatgtatgg
      481 atgcgcactc tcggtgcacc cttcaacgct tccgaggccc gcgtaatgtc ttccggacga
      541 gcccgcgcga ggctgcatac caccggcccc tcgacttcca aggcgatccg acgcaccgct
      601 tcgaaatctc cgggcgagct gtaggcgaac ccggcctcaa taatatccac gccgaggcgg
      661 gccagctgct tggcgatcat aagtttctct tcgacgttca tgctcgcccc tggcgactgc
      721 tcgccgtccc gcaacgtcgt gtcgaatatt ctgatcatcc gagtcatgac aaccctcctc
      781 tgtcctgcac cgtgctcaaa cgggttttcc aacgcggccg caggcgacga aaacaccgga
      841 ggcgtacccg ctggggtacg tcgaggatgt tttcaagccg agaacaaagt tggaaacctg
      901 tttcagcacg gtgccaataa aaaaagcctc cgccccggac agggacgaag gccttgcgag
      961 cttcgtggta ccaccctgat tcagcgccgg atgaagccag ccttcaccgg acaccctcat
     1021 taaatccgtg acgcggatca ttcgggactc attaccacct catcagaggc ttcacaagtc
     1081 cggctcggag gcgagttcag gaagcaaccg gctggttcgc accgtccacc agctctctca
     1141 attcggctac cgctccctac tactcctcgt caatgccgtt acgactctct tgtttcgacg
     1201 accggtccat cagttttcgc gaccggcttg cctgcactct tggcgatcat cgtccagagg
     1261 cgtgcaatcg gacctgacac tgcatacccc gtgaatgtca cgaacagcat agcttgaggc
     1321 cacgcgacaa tcaacatcaa ggccaaaatg ccccagacga gataggtgaa atgatcgcct
     1381 cgccggaact tcatgtcctt gaagctgcga tacttgatcg tgctgaccat cagaaatgcc
     1441 aacccgaacg taatcaccag aatcagcagc ggtttcactt ccgatcccag ctgggtgatg
     1501 tggagatcaa agatcacgag ggaagcaatg acacccgcag ccgccgggat cgctaagccg
     1561 gtaaagtatt tgctgtctga cgtactcacc gtggcattga atcgagcgag ccgcacagcg
     1621 cccatcgcga cataggcaaa catgaccgcc acaccaaaca tgccctgccc gctcagggcg
     1681 taggagtaaa tcagcacccc gggcgcgacg ccgaaggaca ccacatcaga aagagaatcg
     1741 tactcgatcc cgaactgacc ggtgctgttc gtcaatcgcg ccagcttccc gtccaacatg
     1801 tcgaaaatca taccgacgag gatcgcgatg gcggcgacta aatgctgccc gttgaatacg
     1861 gacagaatgg caaacacgcc gcagaataaa ttgcccgtcg tacaaaggtt gggaatcagg
     1921 tacatcgcct gccgcttccg gtgccccttc gcaaacggag gtcgcatagt tggtgatttc
     1981 atcggagttc ccccacgatg gtttcgcccc ctttaacgcg gtcgcccaac gcgacgcaaa
     2041 tcttcgaccc gagcggaaga aaggtatcca ttcgcgaacc gaaccgtatc aacccgaacc
     2101 gttcaccacg ctgcacgcga tcgccttgcc cgacccagca gacgatccgg cgggcaatca
     2161 gccctgcgac ctgcacgcac aggactttcg cccctgactc ggtcttgagc atcacggcat
     2221 tctgctcgtt atgcagcgtc gcttccggct tgctcgccac aaggaactga ccgggctgat
     2281 aattcacccc ttcgacggtt ccagcacaag gcatccggtt cacatggacg tcaaacacgt
     2341 tcagaaatac cgtgacccga atgctttttt ccttgagata ccggggttcg aattcttctt
     2401 cgatcgcaat gaccttgcca tctccggatg agacgaccac attgggttgc tgtggaatgg
     2461 tccggctggg attccggaag aaccaggcgg taaatagcaa aaaaccagcc gctacgaccg
     2521 tgggtatggt ccaccctgcc aacccgaaca acagggtggc gcctgcgaga ccaccgacaa
     2581 acggcaagcc ttctttgaca atcggaattc cgacggctcg atcagccata cccctccagg
     2641 ccctctatca tgctaccacg aagaatccgc cggttgagtt agtttttgtt cttatcgacg
     2701 agttggtcct tcttgagcca gggcatcatg gaccggagcc ggccgccgac ttcttcgatt
     2761 ggatgcccct cgcccttttt caacaaggcg ttatagacag ggcggtttgc ctggttctcc
     2821 aacacccatt ctttggcaaa ctgtccactc tgaatctcgc cgagaatctt cttcatttcc
     2881 tgctttgtct gctccgtcac gacacgcgga ccacgggtaa tgtccccata ctttgccgtc
     2941 gtgctgatcg aatagcgcat gttggcaatg ccgccctgat aaatcagatc gacaatcagt
     3001 ttcacctcat ggagacattc gaaataggcc atctcaggcg cataccccgc ctccacgagc
     3061 gtttcgaatc cggcttggat cagagacgtc agcccaccgc acaggaccgc ctgctctcca
     3121 aagagatccg tctcggtctc ttctctgaaa ttcgtctcga tgatgccggc ccgtccgcca
     3181 cccaccgcgc tggcgtaagc caagccgacc tgccgggtgt tcccgctggg atcttggtgg
     3241 acggccaaca agcatggcac tccgctgcct ttcgtgtatt cggaccggac caggtggccg
     3301 ggccccttcg gcgcaaccat gaacacgttg acgtctgccg gcggcacgat ctgtccgaag
     3361 tggatattga atccatggcc gaacgccaga taggaaccgg gcttcagatt gggcgcaata
     3421 tcctggcgat agatcgccgc ttgcgcttca tcaggggcaa gaatcatgat gacgtccgag
     3481 gctttcacag catcggcaac cggcatgacc ttcaaaccgc tagcctccgc ttttttccat
     3541 gagcttccct cgcgcaaccc caccacgacc gtggcgccgc tttctttaag attgagcgaa
     3601 tgcgcatggc cctggctccc atagccgatc acggccactt tctttccgcg gatgagctgc
     3661 aagtcggcgt ccttgtcgta ataaatcttc atacgtcact cctgaatgtt catagggcac
     3721 atggatggaa acaatcgtgc acagagagga tgcgcgcgtt attctcgagc gagcttcttc
     3781 ggttggctga gggccgggcg gatcgcctca cgcgcgatcg ctactcgccc cgtccggatc
     3841 agctccttga tgccgagcgg ctggagcaag ttgatgatcg cctcaagctt tttcggatcg
     3901 cccgtgacct caatggtata ggtcgatggt gtggaatcaa tcacattggc gcggaaaatg
     3961 tccgcaatcc gaagcgcctc ggcccggtct tccgccttgg tatggacttt aatcaacgcc
     4021 gtctcgcgcg aaacgaactc gctttcattg aggtccacga ccttgatgac gtcgatcagc
     4081 ttgttgagct gcttcacgat ttgctcaacg attcgctcat cccctgaggt gacgatggtc
     4141 atctgcgaca tggacggatc gagggtcgga gcgaccgaca gactttcgat attgaatccg
     4201 cggccgctga acaatccggc cacgcgtgat aacacgccaa acttattttc aacggtgact
     4261 gaaatgatat gttccatcac tatgctctat ggtgctagct gtcaggctga cagcgtgttt
     4321 tacgcggtta aaatggtgtc tttgttttca tccgatccgg tactgggcac accggccatt
     4381 cgctgtttca actcaggcgg gtccgcaaga atcatctcgt gattgcagcc gcccgccggg
     4441 atcatcggat aacaattctc atagggatag gtgggcacat ccacgaccac tggcctgtcg
     4501 gtcgagatgg cctccttcag caccgcatcg aggtctccca ccttaccggc gcgtagtccg
     4561 actgcgccat aggcttccgc caatttcaca aaatccggcg tggtgtccaa gtagctggag
     4621 gcataccgcc cttcgtagaa caggtcctgc cactggcgaa ccatgccgtg gaaacgatta
     4681 ttcagaatga tgattttgac cggcagcttc gacaccaccg cggtggccat ttcctgcata
     4741 ttcatctgaa tgctgccgtc tcctgccaca caaagcacca atcgatccct gaaggcagct
     4801 tgggccccca tggcggcagg aaagccgaat cccatcgtgc ccaatccacc cgaggtcaac
     4861 cagcgattcg gccgggccag tttgaaatac tgcgccgtcc acatctgatg ttgccccacg
     4921 tccgtcgcca cgatcggatc acgatccttc gtcagttcat acaaccgctt gacgacatgc
     4981 tgcggcttga tcgccccgtc cggctcttgt tgataggcca gcggatgcgc ctgctgccat
     5041 tcacggattt ggtcccacca gggcttgcga agatctttct gattgccgtt gaccgtggcg
     5101 cggagaattt ggttcaactc ccgcagcacc gccttgcaat cacccacgat cgggatatcg
     5161 acatgaatgt tcttacggat cgaggttgga tcgatatcga tatggatcac cttggcgaac
     5221 ggacaaaact ccgacacctt cccggtgacc cggtcatcaa agcgcgcacc cacggcgatc
     5281 accagatccg aataatggac cgccatgttg gcgcagtagg tcccgtgcat gcccaacatg
     5341 cccatcgatt ggggatgctc gccgggaaac gcgccgaggc ccattaaggt catatcgaca
     5401 gggatctggg tcatctctgc caattcaagc aactctttcg acgcctgcga aaatacgact
     5461 ccgccgccga catacagaat cggcttcttc gccttcatga tcgcttcagc ggcctgcttg
     5521 atctgccatt tattgccttc gtaggtcgga ttgtatccgc gaatggagac agagctcgga
     5581 taggtgaaat cggccttggc catggacaca tctttgggaa tatccaccag caccggtccc
     5641 gggcgcccgg ttgtcgcaat gtagaacgct tccttaatgg tggtcgccag atcgttcaca
     5701 tccttcacca ggaagttgta cttggtgcag ggacgactca ggccgatgtt gtccgcttcc
     5761 tgaaatgcat cattgccgat caagctcgtg gacacttgtc cgctgataca caccagcggg
     5821 actgaatcca tataggcatc ggccaatgcg gtaatcacat tggtcatacc gggacccgac
     5881 gttactaaac agacgccggc ctttccggtg gccttggcat acccttcagc catatgcccg
     5941 gcaccctgtt cgtggcgggt caatacgact tcaatatctt tctgctggtg cagcatgtcg
     6001 aaaatcttca acacgacgcc gcccggcaag gcgaaaatcg ttttcacgcc ttcagctttt
     6061 aagcattcga tcaggatttc ggaccctgtg agcttcatat caccctcccc ttcgcgcatc
     6121 attagcgaag gacgtgtgcc gtcactggaa acagcagcaa cctgcgtcac gtttgtgtca
     6181 cagactgcta cgatctttct gaaaaatcaa tgggtaagat ggcggatcgt atcaccagct
     6241 cgcaagtgag gtcaagagag tcacgacagg aactcgttgt gcgacagact agtgtcgatc
     6301 accgccccgt ggtataggaa cgttctcgtg ttgtgagcct agtgccaatg agtcccatgc
     6361 gcctcacgat catgtcccgt cgcgaatgtc acttgtgcca tgtggtggcc cgagtggcag
     6421 cccaggtgca ggaggatctc gcgttccatc tgaccatcgt tgatatcgat accgatgaac
     6481 agctccggcg gcaatacgga gaccgcatcc ccgtggtgct attggatgac cgcgaacttc
     6541 tgtccggcaa ggtcactgcg ggagatctac ggaaggcgat aaaaaaagcg cgttggagag
     6601 accctataag ccggattctg tcccgtataa agcttgcgct tacacggggg tgatcatttc
     6661 tctgggactc gagttgcccc gagcctcaag caacctaccc ggagacttcg gccgggccag
     6721 cctttccgct gagctcttgc gagcccgcgg gcgtccccct atttggtctt gctccggacg
     6781 acgcttaccg tgccgttgat gtcgccatca ccgcggtggg ctcttacccc gccgtttcac
     6841 ccttacctga gccgccggga gtgaccgaag tccctcacgc ggccatcggc ggtttgtttt
     6901 ctgtggtgct ggtgtcggat cgctccgcct ggacgttatc cagcgtcctt gcccttggag
     6961 tccggacttt cctcccg
//
LOCUS       DAOQEW010000193         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4030_length_6862_cov_6.909652, whole genome shotgun sequence.
ACCESSION   DAOQEW010000193 DAOQEW010000000
VERSION     DAOQEW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_6862_cov_6.909652"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..3062
                     /locus_tag="PLS35_16185"
     CDS             327..3062
                     /locus_tag="PLS35_16185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI20260.1"
                     /translation="MQRIRPRQMSPLRDEGPGSAHTILSDGTTALLRLAQPVDAEELQ
                     RFFSHLTPAATRHRFFSESAPPAEVIRALCDPSHPERSLTVIVLRRHDDTLRIIASGS
                     YHARGPHEAEVAMAVDDALHGHGLGTILLERLALLAIRQGFTKLWAITHADNLAMREV
                     FATSGFTMEEHLEGGDMEVELSLTPTDQSVRQSEWRERVATIASLHPLFHPRAVAVIG
                     ASRAPRSIGYRLLDALHHNRFEGRCYAINPHASTIAGVEAFPSVRHLPTPVDLAVIAV
                     PKEAVLSVVDECAAIGVRALVVITAGFAEVGAEGRRLQDQLLEKVRLHGMRMVGPNCF
                     GILNTDPAVRLNATFTSTFPCAGSIAMSSQSGALGLALVAASERLHLGLSTFVSVGNK
                     ADVSVNDLLQYWEQDTATNVILLYVESFGNPRRFAQIARRVSRNKPIVVLKAGRTSSG
                     KRAAGSHTAALAANDVAVEALFQQTGILRADTLEEMFALATGLSEQPLPKGRRIGILT
                     NAGGPAILCADACEAAGLEVPELSQATISRLSPFLPASASLRNPVDLIASATPEQYEQ
                     AIVALLTSEDIDALIILYIAVTATDVDPIAQGIARGIEAARAQQLVKKPVSIGWMVET
                     DRDRRFSLPHETIPTYSLPELPARVLGKVAGYVQWRDRPLGMVPDFDDLDISSIKTVC
                     RSVLATRGNGWLTVTETRAVLSSMGISLPPGGLATTAEEAATIAAQIGFPVAVKLASH
                     TLVHKTEVGGVHLNLSSKAEVRRAYDEIAARLAQDHTLDAMEGVLVQSMVTNGVEVMA
                     GMVQDPSFGPLIGFGLGGIHVEILGDVRFRITPLTELDAADLIRSIKGYRLLQGYRGH
                     APGDVDAIQELLLRLSRLVEEVPDIVELDLNPIFALPPGQGYRIVDARIRVAGTG"
     gene            3083..3424
                     /locus_tag="PLS35_16190"
     CDS             3083..3424
                     /locus_tag="PLS35_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20261.1"
                     /translation="MLRLMQAGGSIQSLSAMTEHTLGDATGLMGPAPFVIQPPDYRLL
                     PQRRSGDDSATELMTRGAGVRPCHAKDEFPMGIAACLFRNLSQHPAMLCMATGTGLSL
                     NNERPVPSRHG"
     gene            complement(3671..4258)
                     /locus_tag="PLS35_16195"
     CDS             complement(3671..4258)
                     /locus_tag="PLS35_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20262.1"
                     /translation="MTGVRSRLLAVMTVSVAAMLSALAFEALLSLDKVTPFGHTGYGH
                     GVGWAGLAITLLVFVYPIRKRVNPSHRWPRGWFRVHMVTGVLGPLLIFIHSGAHYHAI
                     VPILAMGSMVIVVVSGVVGQLVHAFSLRALNEHRHDLLHRGLSESDVDAQLHGMASQE
                     EAFRLWQAIHAPMTLMFLAFTALHVVGALFFAGMS"
     gene            complement(4289..4672)
                     /locus_tag="PLS35_16200"
     CDS             complement(4289..4672)
                     /locus_tag="PLS35_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20263.1"
                     /translation="MNRRYAVTLRSLSAVLVLAGLLQGEHAWPDVGIPSSEGLWAPVL
                     RVADNAGTMPNPHSDLIFRATGATRCLDCHRAGKEGAISPQVQDNQLVQDLKAKAKGI
                     HGPGRFADCLRCHAGGNKGVEKYRR"
     gene            complement(4669..5151)
                     /locus_tag="PLS35_16205"
     CDS             complement(4669..5151)
                     /locus_tag="PLS35_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI20264.1"
                     /translation="MSHEKISVTFEQDHDRLDALFTTFQQQKRKDMAKAKEAFVEFKF
                     GLQRHIVWEEDVLFPKWEENSGMAEGGPTQVMRTEHRIIGECLEAIHQKVQANNPDSD
                     LEEQRLVDVLKSHNMKEERILYPSIDQVITDQERAELYQAMKDIPEERYRTCCGSGLA
                     "
     gene            complement(5166..5657)
                     /locus_tag="PLS35_16210"
     CDS             complement(5166..5657)
                     /locus_tag="PLS35_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20265.1"
                     /translation="MSRSKICLGGGLSFLFAMELFTTGCVKQQPAPSADEVRPVHLPE
                     VRTPIPLGPEARQEHRAVMLQHLETIQAIVAALADEDYRLAQGLTESHLGFFMHRYAM
                     ARQQPGDFPPAYHDLAMAHHAAAGKLADVMPTKDLKQILPEFNNVLKACVACHLEYRL
                     RHS"
     gene            complement(5759..6067)
                     /locus_tag="PLS35_16215"
     CDS             complement(5759..6067)
                     /locus_tag="PLS35_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNI20266.1"
                     /translation="MADHDHNCSGLGVSIAFVSGAMLGAMAAILYAPHSGEETRRAIK
                     GYARRTEEDVLEKAKEIRTDIAHTMEEAKRYLKETEATIAAALAAGKEAFKKERADRA
                     "
     gene            complement(6333..6767)
                     /gene="trxC"
                     /locus_tag="PLS35_16220"
     CDS             complement(6333..6767)
                     /gene="trxC"
                     /locus_tag="PLS35_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNI20267.1"
                     /translation="MTESIHLVCPHCRSINRVPAARLAQQPRCGQCHGSLFSGHPIAL
                     TAEDFDLHAQRAEIPLLVDFWASWCGPCRMMAPAYEQAAQMLEPHIRLAKVNTEEEQT
                     LAARFGISSIPTLVLLRGGRELARQPGALGLQDIVRWVRSKL"
BASE COUNT         1350 a         2193 c         1936 g         1383 t
ORIGIN      
        1 ttcaatcggt gaactcgcgc gccaagaccg attgggaatt gttgatcacc atcgcaaacg
       61 gcgcgttatt cagccccatg cacggctacc gcgggaaact gacggatcag caaatgctgg
      121 acgtcttgtc ctatatccgc tccgtcgcca cgccggattt cgtcagctag ccgggcagcc
      181 ttctcggtgg gcgatgctcg actgacattc cccttgggcg gggcagcgga attctggaac
      241 ggcgacttgc aactgtgagg tcgcacggtc atgatcggat ccggcgtctt catgcttctg
      301 accgtggctc gacgcgctgg tagcccatgc aacgtattcg cccccgccaa atgtccccgc
      361 tgcgggacga aggtcccggt tcggcccaca ccatcctcag cgatggtacg acggccctcc
      421 tccgcctggc tcaaccggtc gatgcggagg aactgcaacg tttcttctcc cacctaaccc
      481 cggccgcgac acggcatcgc ttcttctcgg aatccgctcc gcctgccgag gtcattcgcg
      541 cgctctgcga tccttcgcac ccggaacgca gcctgaccgt gatcgtcctg cgccggcacg
      601 acgatacgct acgtatcatc gcatcaggct cttaccacgc gcgaggtccg catgaagcgg
      661 aagtggcaat ggccgtggac gacgcgctcc atggacatgg tctcggcacc attcttctcg
      721 aacgcctggc gctgttggcg atccggcaag ggttcaccaa actctgggct atcacccatg
      781 ccgacaatct ggcgatgcgc gaagtgttcg cgacgtccgg cttcaccatg gaagaacatc
      841 tcgaaggcgg tgacatggag gtcgagttgt cactgacccc gaccgaccag agcgtgcggc
      901 aatccgaatg gcgtgagcgc gtcgcgacca tcgcttctct ccatcctctg tttcatccgc
      961 gcgccgtcgc ggtcatcggc gcctcacgcg cgccgaggag tatcggatac cggttactcg
     1021 acgccctcca tcacaatcgg tttgaaggcc gctgttatgc gatcaatccc catgcgtcca
     1081 ccattgccgg ggtcgaggcg tttcccagcg tgcgccacct tccaacaccg gtcgatctcg
     1141 ccgtgatcgc cgttcccaaa gaagccgtgc tatcggtggt cgacgaatgc gctgccatcg
     1201 gggtccgtgc gctggtcgtt atcacggccg gattcgccga agtcggagca gaaggacggc
     1261 ggttgcagga ccagttgctg gagaaagtcc ggctacacgg gatgcgtatg gtgggaccca
     1321 actgtttcgg cattctgaac acggatcccg ccgtgcgatt gaatgccacg ttcacgtcta
     1381 cctttccgtg cgcgggctcg atcgccatgt cgtcgcaaag cggcgccctg gggctcgcgc
     1441 tcgtcgccgc ctccgaacga ttacacctgg ggctttctac cttcgtcagc gtcggcaata
     1501 aagctgatgt gtccgtcaat gatctgctgc agtactggga acaggacacc gcgaccaacg
     1561 tcatcctgtt gtatgtcgaa tcgttcggca atcccagacg atttgcacag atcgcccggc
     1621 gagtgagccg caacaagccg atcgtcgtgc tcaaagcggg gcggacctca tcaggaaaac
     1681 gagccgcagg gtcccacact gccgcactgg ccgccaatga tgtcgcggtg gaagccctct
     1741 ttcaacagac cggtatcctg cgcgccgaca cgttggagga aatgtttgcc ctggcgaccg
     1801 ggctttccga acaaccgctg ccgaagggac ggcgtatcgg gatcctgacg aatgccggag
     1861 gaccggccat tctctgcgcg gatgcctgcg aggcggccgg cctcgaggtg cctgaattgt
     1921 cacaagccac tatttcccgg ctgtcgccgt ttctccccgc ctctgcctca ctgcgcaatc
     1981 cggtggactt gatcgcctcc gctacccccg agcaatacga acaagcgatc gtcgcgctat
     2041 tgacctccga ggatatcgat gccctcatca ttttatacat cgcggtcacg gcgaccgacg
     2101 tggaccccat cgctcaagga attgcgcgag gaattgaagc cgcacgggca cagcagctgg
     2161 tcaagaaacc ggtctcgatt ggatggatgg tggaaacgga tcgcgaccgc agattttcgc
     2221 tcccgcacga aaccattcct acgtacagcc tgcccgaact ccctgcgcgc gtcctgggaa
     2281 aagttgccgg ctacgtccag tggcgcgatc gtcctctcgg catggtgccg gacttcgatg
     2341 atcttgacat ctcgtccatc aaaaccgttt gtcgcagcgt gctcgccacg cggggaaacg
     2401 gctggttaac ggtcacggaa acacgtgcgg tcctcagcag catggggatt tctctgccgc
     2461 caggaggcct ggccaccaca gctgaagaag cggccacgat cgccgcgcag atcggatttc
     2521 ccgtcgccgt caaactggcg tcgcatacgt tagtccacaa gaccgaggtc ggcggcgtac
     2581 atctcaatct ttccagcaag gctgaggtcc gccgggcgta cgacgaaatc gccgcacggc
     2641 tcgcgcagga ccacacgctc gacgccatgg aaggggtgtt ggttcagtcg atggtcacaa
     2701 atggcgtcga ggtgatggcg gggatggtgc aggatccttc gttcggcccc ttgatcggct
     2761 tcgggctcgg cgggattcac gtcgaaattc tcggggacgt acgattccgg atcacgccgt
     2821 taacagaact ggacgccgcc gacctgatac gcagtatcaa gggctatcgt ttgctccagg
     2881 ggtatcgggg ccatgcaccg ggcgacgtcg atgccatcca ggagctccta ctgaggctct
     2941 cacggttggt tgaagaggtg ccggatatcg tggagctcga tctgaacccg attttcgcct
     3001 tgccgccagg ccagggctat cgaatcgtgg atgcacggat cagagtagcc ggaacgggct
     3061 gagactggca ggtgagaagg gcatgctgcg actcatgcag gcaggcggct cgattcagtc
     3121 actctccgct atgacagagc atacactggg cgatgcgacc gggttgatgg gccccgcgcc
     3181 ctttgtcata caaccgccgg actatcggct cctgcctcag cgtcggagtg gtgacgattc
     3241 ggctaccgaa ctcatgacac gcggtgcagg agttcgtccc tgtcacgcga aagacgaatt
     3301 ccccatgggg attgcggcgt gtctgttccg taatttgagc cagcatcccg cgatgctctg
     3361 tatggcaacc ggtacaggct tgtcgttgaa caacgagcgg ccggtgccat cccgccacgg
     3421 ctgacgtggt acgagcttcg aaatcgccct cgttatggca gcctgtgcag cgcgcggaac
     3481 tgggccctcg aaacggctca tggcactgcg tacacccgcc ttgctccgca tgataccggc
     3541 taaggtcccc cggggcccag aaggccgcgg gactccgcgc ggtgccccac ccgatccaca
     3601 tcaccagtat cagcgtcgca agcgccacca ccggcactgc agttgttggc agcggatgat
     3661 agcgcatggc tcacgacatt ccggcaaaga acaaggctcc caccacatgc agggcggtga
     3721 acgccagaaa catcaatgtc atcggtgcat gaatcgcctg ccagagacga aaggcctctt
     3781 cctgggaagc catcccatgc aactgcgcgt cgacgtcact ttccgacagt cctcgatgca
     3841 gaaggtcatg ccgatgttca ttgagcgctc gcagactgaa ggcgtgcacc aactggccga
     3901 ccacgccgct gaccaccacg atgaccatgg agcccatcgc gaggatcgga acgatcgcgt
     3961 gatagtgggc accggagtga ataaaaatca gcagcggtcc gagcacccct gtcaccatat
     4021 gaactcgaaa ccaaccacgc ggccatcggt gtgagggatt gacccgtttt cggatgggat
     4081 aaacgaacac cagcagggtg atggccaggc ctgcccagcc gactccatgc ccataccctg
     4141 tgtgaccgaa tggcgtaacc ttatccagac tgagcagcgc ttcgaacgcg agcgcgctca
     4201 gcatcgcagc gaccgacacc gtcatgaccg ccaggaggcg cgaccgcaca cccgtcatct
     4261 gcgcaatccg ttcttcatgc cccgctgatt accgtcggta cttctcaacc cccttgttgc
     4321 ctccagcatg gcaacgtaaa cagtctgcga accgaccggg gccatggatg cccttcgctt
     4381 tggccttgag atcctgcacc agttggttgt cctgtacttg cgggctgatc gctccctctt
     4441 ttccggcccg gtgacaatcc aggcacctgg tggcgccagt cgcacgaaag atcaaatcac
     4501 tgtgcggatt gggcatcgtg ccggcgttat cggcaaccct gagcacaggc gcccatagtc
     4561 cctcagatga tggaatgcct acatcaggcc atgcgtgttc tccctgcagc aagcccgcaa
     4621 ggacaaggac cgccgacaga ctcctgagtg tcacggcgta ccgacggttc atgcgagccc
     4681 gctcccgcaa caggttcggt atcgttcctc ggggatatcc ttcatggcct ggtataactc
     4741 cgcccgctcc tgatcggtga tgacctgatc gatcgacggg tagagaattc gctcttcttt
     4801 catgttgtgc gacttcagca cgtcgaccag ccgctgttcc tccagatcgc tgtcaggatt
     4861 gttcgcctgc actttctggt gaatggcttc caggcactcg ccgatgatcc gatgttcggt
     4921 ccgcatgacc tgcgtcggtc ctccctccgc catgccggag ttttcttccc attttggaaa
     4981 caagacatct tcctcccaca cgatgtgccg ctggagcccg aacttgaatt cgacgaacgc
     5041 ttctttcgcc ttggccatgt ccttccgttt ctgttgctgg aatgtggtga agagcgcatc
     5101 cagccgatca tgatcctgtt caaacgtgac actgatcttc tcatgggaca tgccgttctc
     5161 cttcgttatg aatgacgaag cctgtattcc aagtgacagg cgacacaggc tttgagcaca
     5221 ttgttgaact cgggcagaat ctgcttcaga tctttcgtcg gcatcacatc ggccagtttc
     5281 ccggccgcgg catgatgggc catcgcgaga tcgtgatagg ccggcggaaa gtctcccggc
     5341 tgctgcctgg ccatagcata ccggtgcatg aaaaatccca gatgcgattc tgtcagtccc
     5401 tgcgcaagac ggtaatcctc atccgctaaa gccgccacga tcgcttgaat cgtttccaaa
     5461 tgctggagca tgacggcgcg atgttcctgc cgcgcctccg gcccaagagg aatcggcgta
     5521 cgtacctcgg gcaaatgcac gggcctgacc tcgtcagcag acggcgccgg ctgctgcttc
     5581 acacacccgg tcgtgaagag ctccatcgca aagaggaatg agagcccgcc tcccagacaa
     5641 attttgcttc gactcatgac tgccctctat cgcctgttgc cgataggcat ccaccggcgg
     5701 gatagtgcac ccggcaaggc gatgtcgccc tccgcacggc gttcgcacag cgaccttctc
     5761 aggctctgtc tgcccgctcc ttcttgaaag cttcctttcc agcggccaat gcggcggcga
     5821 tcgtggcttc tgtttccttc agatatcgct tggcctcttc catcgtgtgt gcaatgtccg
     5881 tccggatttc tttggccttc tccaacacat cctcttccgt ccggcgcgca tagcccttga
     5941 tcgcccggcg ggtttcctcg ccggaatggg gcgcatacag gatggctgcc attgccccga
     6001 gcatggctcc actcacaaat gcgatcgaga cgcccagccc ggaacaattg tgatcgtgat
     6061 ccgccatgat gacctcccct ctccccctct atgtttttag aatctgagac ttcccgcttt
     6121 gtctttatag aaagcagagg acgtgccagg ccagcgtgat gcgggagcag acaagtcaca
     6181 attttgacaa gggtttcaca gtgactgcga caggttgcgt tcccgcggcc tgtccgtttg
     6241 gggaattaca gaaccgggcg agaatcaaca gtggcgcaaa acgcgccagg tgagagacgc
     6301 gtcgactttg gaaggattag aaattctggc tatcagagct tactgcggac ccaccgcaca
     6361 atgtcctgaa gacccagcgc ccccggctga cgagccagtt cacggcctcc gcgcagaagc
     6421 accaatgtcg ggatactgga aataccgaac ctcgcggcca acgtctgctc ttcttctgtg
     6481 tttactttgg ccagccggat gtgaggttcc agcatctgcg cggcctgctc ataggccggg
     6541 gccatcattc ggcaggggcc acaccaagag gcccagaaat cgaccagcaa aggaatctcg
     6601 gcccgctgcg cgtgcagatc aaaatcctcc gccgtcaagg cgatcggatg ccccgaaaac
     6661 aaggacccat ggcattgccc acaacgaggc tgctgcgcca gacgggccgc gggcactcga
     6721 ttgatgctcc ggcaatgcgg gcacaccaag tgaatcgatt ccgtcacagc acaacctgct
     6781 tgccgcaaga caccgcgcca ttaccgatca caccgttccc cttcacagcg cccgctcccg
     6841 cttcccatgc ctcgatggcc ca
//
LOCUS       DAOQEW010000194         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4111_length_6786_cov_9.448076, whole genome shotgun sequence.
ACCESSION   DAOQEW010000194 DAOQEW010000000
VERSION     DAOQEW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4111_length_6786_cov_9.448076"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLS35_16225"
     CDS             complement(<1..442)
                     /locus_tag="PLS35_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNI20268.1"
                     /translation="MTSHMLLSVALRSAAVGYVLLLIFLPLATLTQQSIASGWDRFIQ
                     DLSAPQAAAALWLTIETSAIATAINAVFGTLSALVLVRYEFPGRWLLNALVDLPFAIP
                     TLVAGLMIAAIYGPTSVLGTWLQQGGLTVLYNQPGIILAMLFVTM"
     gene            complement(704..1768)
                     /locus_tag="PLS35_16230"
     CDS             complement(704..1768)
                     /locus_tag="PLS35_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI20269.1"
                     /translation="MRTITHTFSTLMLSVIASWGLFTFPVAPSHAAETRDLILAAYSV
                     PKEAYERHIIPAFQRYWKQKTGQDVRVRSSYGASGAQARAIIGGFDADVAVLSLEGDV
                     DQITKAGLITHDWRKAPHGGMISASVVALGVRKGNPKGVKGWEDAARPGVEVLYPNPK
                     TSGGAMWDVIAIYGAGLKLAEQRAGKPVAPEAAATQAVDLLTRIQRNVKVMDKSGRES
                     VTTFERGVGDVIVTYENELLPRVKSGRPYELIVPAETVWIENPAAVVDTYADRHKAKD
                     LAEAFVAFLHGPEAQAAFIELGFRPLDRGAVAPASGAALPQPARLFTIADLGGWESVS
                     TKLFGAQGVWTKIVEDVSKK"
     gene            complement(1768..2208)
                     /locus_tag="PLS35_16235"
     CDS             complement(1768..2208)
                     /locus_tag="PLS35_16235"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNI20270.1"
                     /translation="MKFSKKSEYGLRALLELCETYGGRVLQRHEIAERQNIPVEFLEQ
                     ILLALKRAGLLASRRGIRGGDSLIKSPEEITLGQVIRILDGPLAPISCVSKTAYQKCS
                     DCPYAAKPSCPLQQAMGEVRDAIANILDHYTLSRFAHTKLAEGS"
     gene            2453..3373
                     /locus_tag="PLS35_16240"
     CDS             2453..3373
                     /locus_tag="PLS35_16240"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNI20271.1"
                     /translation="MNFQDLILTLHRFWADRGCVIHQPYDLEVGAGTFHPATFLRSLG
                     PEPWSAAYPQACRRPTDGRYGENPNRMQHYYQYQVVLKPAPDNIQGLYLESLKQLGID
                     PKKHDIRFVQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGIPLNPITGEITYG
                     TERIAMYLQQVDNVYDLQWSDGVTYGDVHHRSEVEFSRYNFEEGEVPMLMATFQAFEG
                     ECQRLLDKKLTLPAYDYCIKTSHMFNLLDARGAISVTERTSYIARVRALARRCAESYV
                     ADREAMGHPLIKQPAGAGKGTINHSPVTTK"
     gene            3395..5596
                     /gene="glyS"
                     /locus_tag="PLS35_16245"
     CDS             3395..5596
                     /gene="glyS"
                     /locus_tag="PLS35_16245"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNI20272.1"
                     /translation="MPSKKPKTKTVSRAKKPASASRAKTPSTTELLLEIGTEELPYQF
                     VAPALRALQQGAETLLKDSRLTYGTVRTMGTPRRLVLLVEGLARQQASAVKEAMGPSK
                     AVAFDQSGQPTRAAIGFAAGQGVPVESLQVRQTPKGDYLFAVKPEKGQAVAAVLTQAL
                     PQLLAKLSFPKAMHWNQTGVRFARPVRWLIALCGGKVLPIQFATIKAGNYSQGHRVLG
                     AKVSSPKGFAVQSIAHYFKETERHSVIVDQDRRRAMILDQLAALAKSARGHLHQDEEL
                     LEQAVYMVECPLTILGSFKPHYLALPKEILMTSMKEHQGYFSLVDSKGALLPNFLAVT
                     NMKLANMQLIREGNERVLAARLADAKFFFDEDRKTSLADRVAKQLAVTFHQKLGSLHQ
                     KTQRVVAMAAHLAGQLGDDQLVHDCRRAAELSKADLLTGIVGEFPTLQGMMGGEYAKH
                     DGESATVSAAIREQYLPRAMEGELPESLAGKVLSLADRLDSIAGFFLVGLVPSGSEDP
                     FALRRHATAIVRILIESRLQINLAQAVRATQEGLAGQHVAAAPQPAKGGAPDVIGFLF
                     ERVRFYGKSAHQLRDDVMEAVLKSADRQSIDLTDLFDKMQSLQQITSRAEFDPLIVGF
                     KRAHRLTEKEQWDRKPVESGLFREPAESALYETVQQSHAVYAAAMRGGQYGQALDVLV
                     RMKGPIDDFFNAVMVNADDPAVRGNRLSLLKEVDDLFMSFADFSQIMVQGT"
     gene            5716..6129
                     /locus_tag="PLS35_16250"
     CDS             5716..6129
                     /locus_tag="PLS35_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20273.1"
                     /translation="MMVVCNAVFLFGIWVTGLNFERLVRTPDIYDSRQDICLRLAYQH
                     VPGSENPVQFCSEWLNLADPTGKTHTFQKDAQLKQGADGSFYFDYGPFVDYRLFAVGA
                     FVVGIIVCGIRVTRHVVNRYRMRLEAAARHSVPTH"
     gene            6155..>6786
                     /locus_tag="PLS35_16255"
     CDS             6155..>6786
                     /locus_tag="PLS35_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNI20274.1"
                     /translation="MAKKYVYYFGDGKAEGTGNMKELLGGKGAGLAEMTNLKVSVPPG
                     FTISTEACVEYYKRGKAYPPGMMEEALVALKKIERSMKAGFGDPDSPLLVSVRSGARA
                     SMPGMMDTVLNVGLTTKTVHGLALKTKNERFAQDSYRRFIGMFGSIVMGINREHFEDI
                     LKHKKRDLGVTQDTHLDAKALKELVVSFKELVKEETKRDFPDDPLEQLRMA"
BASE COUNT         1418 a         1941 c         2041 g         1386 t
ORIGIN      
        1 gcatggtgac aaacagcatg gccagaatga tgccgggctg gttatacagc accgtgaggc
       61 caccttgctg gagccaggtg ccgagcacgc tcgtgggccc ataaatggcg gcaatcatca
      121 gcccggcgac gagagtggga atggcgaagg gcagatcgac caacgcattc aagagccagc
      181 ggccgggaaa ttcataacgc accagcacga gtgccgaaag agtgccgaag acggcattga
      241 tggcggtggc gatcgcggat gtttcaatgg tcagccagag ggcggctgcc gcttgagggg
      301 ccgataaatc ctgaatgaaa cggtcccatc ccgaggcgat ggattgctgc gtcagggtag
      361 ccagcggaag aaagatcagc agcaggacat accccaccgc tgcagaccgc agtgccacac
      421 tcaagagcat gtgtgaggtc atgcgcccgt tccgtgtctg tggtgacggt catttatgtt
      481 tggcatgcaa ctatttccac ttgtggtggc gatcatgtgt tactggttca ttcacaggag
      541 gctcaacagg tcagtccagc aaggccgcag cgagtgaagg cctgagctgc atgttcttac
      601 ccgcccaccc tgagcctgcc gagacagact catgtcccat gggcggtacg ttgagggcct
      661 gaacgatgcg agaacgccgc tggctggcct gttcagcctc ctgctatttc tttgacacgt
      721 cttcgacaat cttcgtccac acgccctgtg cgccgaagag tttggtgctc acggactccc
      781 atccaccaag gtctgcaatg gtgaacagcc gggccggttg aggcagggcg gcacccgaag
      841 caggcgccac tgcgccacgg tccaacggac ggaatcccag ttcgatgaac gcagcctgcg
      901 cctccggacc atggaggaaa gcgacgaacg cttcagccag atccttggcc ttgtgccggt
      961 cggcataggt atcgaccacc gccgccgggt tttcgatcca caccgtttcg gccggcacaa
     1021 tgagttcgta ggggcgcccg cttttcacgc gcggcaacaa ttcattttca taggtcacga
     1081 tcacatcgcc gacgccgcgt tcgaacgtcg tcaccgattc ccggccgctt ttgtccatga
     1141 ccttcacgtt gcgctgaatg cgcgtgagca agtcgaccgc ctgcgtcgcc gccgcttccg
     1201 gagccaccgg ttttcccgcc cgttgttcgg cgagtttcag ccctgcgcca taaatggcga
     1261 tcacatccca catggcaccg ccggaggttt tgggattggg atacagcact tcaacgccgg
     1321 gccgtgccgc atcttcccag cccttcacgc ctttgggatt gcctttccgg acaccgagcg
     1381 ccacgaccga ggcggagatc atgcctccat gcggagcttt cctccagtca tgcgtaatca
     1441 ggccggcttt ggtgatttga tcgacatcgc cttccagcga gagcacggcg acatccgcat
     1501 cgaaccctcc gatgatggcg cgcgcctggg ctccggacgc cccgtacgaa ctgcggaccc
     1561 gcacatcctg tcccgtcttc tgtttccaat accgttggaa ggcgggaatg atgtgtcgtt
     1621 cataggcttc cttgggcacg ctgtaggccg ccagaatcaa atcgcgcgtc tcggctgcgt
     1681 ggctcggtgc aaccgggaac gtgaacagac cccaggacgc gatgacgctg agcatcaggg
     1741 tcgaaaatgt atgggtgatg gtacgcatca ggatccttcc gccagtttgg tatgggcaaa
     1801 tcgactcaag gtgtaatggt cgagaatgtt ggcaatggca tcgcgcacct cccccatggc
     1861 ctgctggagc ggacaggagg gtttagcggc gtaaggacag tcggagcatt tttgataggc
     1921 ggttttgctc acacagctaa tcggggccag tggcccgtcc agaatccgga tgacctgccc
     1981 gagcgtgatt tcttcagggg atttgatcag tgaatccccg ccgcgaatgc cgcgccgact
     2041 cgccagcagc ccggcgcgtt tgagggcgag cagaatctgc tcgagaaatt ccacagggat
     2101 gttctgccgc tcggcaatct catggcgctg gaggacgcgc cctccatacg tctcgcagag
     2161 ctcgaggagc gcccggagcc cgtattcact ctttttggaa aacttcatgg tcgaggcagt
     2221 tgtctagtga gttgctcaag atttggcaga ataaaccagc gcgtctcgcg attcaagcga
     2281 ttcgaccacg aggtggcgaa gaagaaatcc gttccactgc gtagccggtt ccatcctgca
     2341 acctattgaa aacggacgct ccgtttcttg acaccctgta accgttcttg ctagcttcag
     2401 cgtgattttt gtccgtcacc acgagcagcc tgcggctaga tctggacatc cggtgaattt
     2461 ccaagacctc attttgactc tgcatcgctt ctgggccgac cgtgggtgtg tcatccatca
     2521 accatatgat ttggaagtgg gtgcgggtac cttccatccc gctacgttcc tccgttccct
     2581 ggggcctgaa ccctggtccg ctgcgtatcc gcaagcctgc cgccggccga ccgatggccg
     2641 ctacggtgaa aatcccaacc gtatgcagca ttactaccag taccaggtag ttttgaaacc
     2701 agctccggac aatatccagg gactctatct ggagagcctc aagcaactgg gcatcgatcc
     2761 caaaaagcac gacatccggt ttgtgcagga tgattgggaa tcaccaacgc tgggggcctg
     2821 ggggttgggc tgggaagtgc gtctcgacgg catggagatt acccagttta cgtattttca
     2881 agaaatcggc ggcattccgc tcaatccgat caccggcgaa atcacgtatg gcaccgagcg
     2941 catcgccatg tatctgcagc aggtcgataa cgtctacgat ctgcagtgga gtgacggcgt
     3001 cacctacggc gacgtgcatc accgcagcga agtcgagttt tcacgctaca acttcgaaga
     3061 gggcgaggtg cccatgttga tggccacctt ccaggccttc gaaggagaat gtcagcgact
     3121 gctggacaag aagttgacgc tgccagccta cgattactgc atcaagacct cgcacatgtt
     3181 taacctgctg gatgcgcgcg gggcgatcag cgtcactgag cggacgtcct acatcgccag
     3241 ggtgcgggcg ctggcgcgtc gctgcgcgga atcgtacgtg gccgaccggg aggccatggg
     3301 ccatcctttg atcaagcaac cggctggggc aggtaaggga accatcaacc actcaccggt
     3361 cactaccaag tagcccgcgg atcctatttc cacaatgcca tctaaaaaac ccaagacgaa
     3421 gaccgtttca cgcgcgaaga agcctgcatc tgcctcacgc gccaagactc cctctacgac
     3481 ggaattgctg ctcgaaatcg ggacggaaga gttgccctat cagtttgtcg ctcccgcgtt
     3541 acgcgccttg cagcagggag cggagacgtt gctgaaagac tcacgtctca cctacggcac
     3601 tgtccggacg atgggcacac cgcggcggct cgtgttgttg gtcgaagggc tggcgcgaca
     3661 gcaggcctcc gccgtcaaag aagccatggg gccttcaaaa gccgtggcct tcgatcagtc
     3721 gggacagccg acgagagcgg ccattgggtt tgctgcgggt caaggcgtgc cggttgagtc
     3781 tctgcaagtc cggcagacgc ccaaaggcga ctatctgttt gcggtgaagc cggagaaggg
     3841 ccaggccgtg gctgcggtcc tgacgcaggc gttgccgcag ttgttggcca agttgtcgtt
     3901 tcccaaggcc atgcattgga accagaccgg ggtgcgcttt gcccggccgg tgcgttggtt
     3961 gattgcgctc tgcgggggca aggtcctgcc gattcagttc gcgacgatca aggccggcaa
     4021 ttacagccag gggcatcgag tgctgggtgc gaaggtgtcc agccccaaag gtttcgccgt
     4081 gcaatccatc gcgcactatt tcaaagagac cgaacgccac agcgtgatcg tcgatcagga
     4141 tcgccggcgc gccatgatcc tcgatcaact agccgcgttg gccaaatcag cacgcgggca
     4201 cctgcatcag gacgaggagt tgctggagca ggctgtctac atggtggaat gcccgctgac
     4261 gattctcggt tccttcaaac cgcattatct ggcgctcccg aaagaaatcc tgatgacctc
     4321 gatgaaggag catcagggat atttttccct cgtggactcc aagggcgcgc tgttgcccaa
     4381 tttcctcgcg gtcacaaata tgaagttggc gaacatgcag ttgatccgtg aggggaacga
     4441 acgggttctg gctgcgcgct tggccgacgc gaaatttttc ttcgacgagg atcgcaaaac
     4501 gtcgctggcg gatcgtgtgg caaagcagct cgcggtcacc ttccaccaga aacttggcag
     4561 cctccatcag aaaacgcagc gcgtcgtggc gatggcggct catctcgccg ggcagcttgg
     4621 tgacgaccaa ttggttcacg actgccggcg cgcggcggaa ctcagcaaag ctgatttgtt
     4681 gacgggtatc gtcggcgagt tcccgactct tcaaggcatg atgggcgggg agtacgcgaa
     4741 acacgacggg gaatcggcga cggtcagcgc cgcgattcga gaacagtatc tgccacgggc
     4801 gatggaagga gagttgccgg aatcgctggc agggaaagtg ctctctctgg ccgaccggtt
     4861 agacagcatt gccggatttt tcctggtcgg gctggttccg agcggctccg aagatccctt
     4921 cgccctcaga cgccatgcca cggcgattgt gcgtattctc atcgagagcc gattgcagat
     4981 caatctcgcg caagcagtgc gggcgaccca ggaagggttg gccggtcagc atgtggcggc
     5041 tgcgcctcag ccggccaaag gcggcgcgcc ggacgtgatc ggatttcttt ttgagcgggt
     5101 ccgtttttac ggaaaaagcg cgcaccaatt gcgggacgat gtgatggagg ctgttctgaa
     5161 atcggccgac cgccagtcca tcgatctcac cgacctcttc gacaagatgc agtcgctgca
     5221 gcagattacg tcgagggcgg agttcgatcc gctgatcgtt ggattcaaac gcgcgcatcg
     5281 gctgaccgaa aaggaacagt gggatcgtaa gccggtggag tccgggttat ttcgggagcc
     5341 tgccgagtcc gccttatatg agactgtgca gcaaagtcat gctgtctatg cggccgccat
     5401 gcgcggcggg caatacggac aggcgctgga cgtgctggtt cggatgaagg gtccgatcga
     5461 cgactttttc aatgcggtga tggtcaacgc cgacgatccg gccgtgcgtg gaaaccggct
     5521 gtcactcttg aaagaggtcg atgatttgtt catgtcattc gctgatttct cccagattat
     5581 ggtacaaggg acgtagcgga tggtgagcat ttggactcaa cttcagagag ccaccctgag
     5641 tcacccgagg ccagtgcctc ggctcaagta agagcgacca aagtccggcg atttgcgtcc
     5701 ggcatcagcg tcgtgatgat ggtggtgtgc aatgcggtct tcctgttcgg catctgggtg
     5761 accggcctga attttgaacg gctggtgcgg acaccggata tctatgattc cagacaagac
     5821 atttgcttgc gactggccta ccaacatgtt ccgggctcgg aaaatcccgt gcagttctgt
     5881 tcggagtggc tcaacctcgc cgatccaacc ggaaaaaccc atacgtttca gaaagacgca
     5941 caactcaagc agggtgcaga cggatcattc tatttcgact acggaccgtt cgtggactat
     6001 cgtctgtttg cggtcggcgc gttcgtcgtg gggatcatcg tgtgtggcat tcgcgtgacc
     6061 aggcacgtcg tcaaccgcta tcgtatgcga ctggaagccg ccgcccgcca ttcggttccg
     6121 actcattgac ctcatagcag aaggagagcg tcgcgtggcc aagaaatacg tctattactt
     6181 tggagatgga aaagccgaag gcaccggcaa catgaaggaa ctgctcggcg gcaaaggcgc
     6241 aggtctggcc gagatgacca atctcaaagt gtccgtgccc cccgggttta cgatctccac
     6301 cgaggcctgc gtcgaatatt acaagcgggg caaagcctac cctcccggca tgatggagga
     6361 agccctggtg gcgctcaaga agatcgagcg gtcgatgaag gcgggcttcg gcgatcccga
     6421 cagcccgtta ttggtctccg ttcgctccgg tgcgcgtgct tccatgcccg gcatgatgga
     6481 taccgtgctg aacgtgggat tgaccaccaa gaccgtgcac ggcttggcgc tcaagaccaa
     6541 gaacgaacgg tttgcgcagg atagttatcg ccgcttcatc ggcatgttcg gcagcatcgt
     6601 catgggcatc aatcgcgagc atttcgagga cattctcaaa cataaaaagc gcgatctcgg
     6661 ggtcacgcag gatacgcatc tcgatgcgaa ggcgctcaag gaactcgtcg tcagtttcaa
     6721 ggaactcgtc aaagaggaaa ccaagcgcga ttttccggat gatccgctcg aacaactacg
     6781 catggc
//
LOCUS       DAOQEW010000195         6733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4165_length_6733_cov_8.386044, whole genome shotgun sequence.
ACCESSION   DAOQEW010000195 DAOQEW010000000
VERSION     DAOQEW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4165_length_6733_cov_8.386044"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..801
                     /locus_tag="PLS35_16260"
     CDS             88..801
                     /locus_tag="PLS35_16260"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNI20275.1"
                     /translation="MARLGVNIDHVATLRQARGGTDPDPLTAAILVELAGADGLVVHL
                     REDRRHIQDRDLTILREIVRTKLDLEMAADDAMAKIALSVKPDLVTLVPERRQELTTE
                     GGLDVANHRERIQKIVDLLRDGGIPTSLFIEPSLDQIKAAHKIGAAYVELHTGRYANA
                     NRSKEEDEEFEAITQAAKLAYKLGLGVNAGHGLTYKNVKRLAKLPEIVEFNIGHSIIA
                     RSVMVGLVQAVREMKELVA"
     gene            861..2417
                     /locus_tag="PLS35_16265"
     CDS             861..2417
                     /locus_tag="PLS35_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNI20276.1"
                     /translation="MIPIVTAEQMRALDHRTITEAQVPSLVLMERAGTGVVTHLEARY
                     GSVAGKKIVILCGKGNNGGDGFVVARLLRRKKARVHVLLLAPVAELSRDAKAMYQRFH
                     RTAGPSSITRPSDTNAIQQRLTPSEIIIDAILGTGLSTPVTGLYRDAIDAINAAGRLT
                     VAVDLPSGLHADTGSVLGTAVKADLTVTFGLPKAGLYCGAGIDCAGLVRLADIGIPSS
                     FVDAVGSRLLLLTAASANAALPPRHPSSHKGTYGHLGIIAGSVGKTGAAAMAAMAALR
                     IGTGLVTAAVPSSVNDILEAKLLEAMTLPMPETKARTFARSGLDRLLAFAGPRDAVAI
                     GPGLTTHPETVDLVQEFVKRIDKPCVLDADALNALAGKASLLTECPRPPIITPHPGEM
                     ARLEAEATTQSVNEDRLGTATRFARERGVFVVLKGARTVIARPDGVAAICPTGNPGMA
                     TAGTGDVLTGMVGGLLAQGVAPWDAACAATYFHGLAGDLAAQQLGQAGMIARDLIRHI
                     PHAIAPTTHL"
     gene            complement(2710..2850)
                     /locus_tag="PLS35_16270"
     CDS             complement(2710..2850)
                     /locus_tag="PLS35_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20277.1"
                     /translation="MGKINLKPIMRKPCAPAFGPFNSENSAAVYVVTQARILELGGTE
                     AV"
     gene            3007..3351
                     /locus_tag="PLS35_16275"
     CDS             3007..3351
                     /locus_tag="PLS35_16275"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNI20278.1"
                     /translation="MIRLILVGTVLLLALSFFLQNQEQEVTLRYFFGLRSASILIYKP
                     ILAAFGVGLLVSGILLFPAWVRGRIELRRKTKALQEAEVDLERLRQALDKAARRVGSS
                     SDIPTEGEPVDG"
     gene            3344..5992
                     /gene="mutS"
                     /locus_tag="PLS35_16280"
     CDS             3344..5992
                     /gene="mutS"
                     /locus_tag="PLS35_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNI20279.1"
                     /translation="MGEADGTPLMRQYREIKQAYRDAILFFRVGDFYEMFQEDAVEAS
                     KLLSIALTSRDKSSETPIPLCGVPYHAATNYIAKLLRAGRTVALCEQVEDPKLAKGLV
                     RREVVRLYTPGTLIDSEFLPSSESNLLAAVAIRLRPATHEPTGCSFGLATLEVSTGEY
                     WLCEFHGPQAQIALMDELARLDPKELLYAEGCTPGEQSWMAELMGPRCCAQPATWFEL
                     DTGRAMLQEHFRVHSLDSFGCQQLTLGIQAGAAVLRYVRETQPSASLDHIRNLRVRQP
                     HDAMHLDSVTIRNLELVKPAGRWDDAPNHRSPYTLLGVLDRTVTAMGSRLLREWLLRP
                     LINCAPIEARLTAVEELKESIEPRVRLRTALRTVQDISRLCSRISLGVANPRDLLALK
                     LSLSSLPAIQAELTPLQAPLLAELSASWDNAQDLYELIEQAILADAPVSIRDGGILKD
                     GYHPDVDELRKATREGKGWIAGLEAKERERTGAESLKIRYNQVFGYYIELTKANLGKV
                     PSDYIRKQTLVNAERFMTAELKELEERVTGADAKLVALEQSLFEQLRTGLAAETARLQ
                     EISRRLAVLDVLTALAETAGLNRYVRPTIDTGDVLHIKQGRHPVVERLDLSGGFIPND
                     THLDLHTSRLHILTGPNMAGKSTYLRQVALIALMAQIGSFVPAAEARIGLVDRIFTRV
                     GASDNLAGGQSTFMVEMTESAHILHCATARSLILLDEIGRGTSTYDGLSIAWAIAEFI
                     QDPQRLGARTLFATHYHEMTQLEGLRAGITNYSVAVKERDGKVLFLRKIVQGGADRSY
                     GIHVAQLAGLPDAVIQRAKAVLAQLESTTAPRPILESQQALQFDSTLPAPHPMLEEVR
                     QMDLFSMTPLDALNRLAALQQRLLSE"
     gene            complement(6099..>6733)
                     /locus_tag="PLS35_16285"
     CDS             complement(6099..>6733)
                     /locus_tag="PLS35_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20280.1"
                     /translation="AALVVSFASTALAIEAFQERFEWGDLSKPTTLQGRVIILDPYDE
                     AVWLNIAVFGGNAESGLWWQRVHPGKNLKFYPADKNVWEQLKSMARAHSGPAAAKEVP
                     PGSPTTLVEFVAQETEQNHRVITSVKKLNDNAGEMGKPKSISSLRLKECEGKTEVDAA
                     CHAAKTLLNTSPKTPDGANLPLQYDVLLPDGKPIANLIPWTAKYNQGSKH"
BASE COUNT         1434 a         2045 c         1861 g         1393 t
ORIGIN      
        1 tgcctcagga acaacagaat tgtctatgaa tttcgaccag ttccgaaatc tttctgcatc
       61 gtcattcatg gtatttgcgg aggcgtaatg gctcgattgg gcgtgaacat cgatcatgtg
      121 gcaacactcc ggcaagcgcg tggcggaacc gaccccgacc ctctgactgc cgccatcctc
      181 gtcgaactgg ccggcgcaga cggtctcgtc gtgcatctcc gggaggatcg ccggcatatt
      241 caagaccgcg atctgacgat attgcgggaa atcgttcgca ctaaactaga tctggaaatg
      301 gccgccgatg acgccatggc caaaatcgcg ctaagtgtga aacctgacct cgtcacgctg
      361 gttccggagc gtcgtcaaga gctgactacg gagggcggcc tcgatgtcgc caatcaccgc
      421 gagcgaatcc aaaagatcgt cgatttgctc cgcgatggcg ggatcccgac cagtctgttc
      481 atcgaaccta gcttggatca aatcaaggcc gctcataaaa tcggcgcggc ctatgtcgag
      541 cttcacacgg gccgttacgc gaacgcaaac cgctcgaaag aagaagacga agaattcgaa
      601 gcgatcactc aggcggcaaa actagcctac aaacttggcc tcggagtgaa cgccggccat
      661 ggcctgacct ataagaacgt caaacgactc gccaaactgc cggagatcgt cgagttcaat
      721 atcggccaca gcattatcgc ccgctccgtc atggtggggc tcgttcaggc tgtccgtgaa
      781 atgaaagaat tggtggctta gcctggcttt ccccaggcaa ggtcctccct cagtcccgaa
      841 tcggaggccc gattgacgtc atgataccga tcgtgaccgc cgagcaaatg cgtgcactgg
      901 accatcggac gatcaccgag gcacaggtac cgtccctcgt actgatggaa cgagccggaa
      961 caggtgtggt gactcatctt gaggcgcgat acggatcggt tgcaggaaaa aagatcgtca
     1021 tcctgtgtgg caagggcaat aacggtggag atggtttcgt cgtcgctcga ctgttacgga
     1081 gaaaaaaagc tagagtccac gtgttgttat tagcaccggt ggctgaactc agccgcgatg
     1141 cgaaggcgat gtatcagcgc tttcaccgga cagcaggccc atcttccatt actcgcccct
     1201 ctgatacgaa cgccattcaa caacggctga caccaagcga gatcattatc gatgccattt
     1261 taggcaccgg cctctccacg cctgtgaccg gcttataccg tgatgcgata gacgccatca
     1321 atgccgctgg acgcctcacc gtcgccgtgg atcttccgtc aggccttcat gccgataccg
     1381 gatctgtcct gggcacggcg gtcaaagcgg acctcaccgt gacctttgga ttgccaaagg
     1441 caggactcta ttgcggagcc ggcatcgatt gcgcggggtt ggtgcgtctt gctgacatcg
     1501 gaattccgtc ttcgtttgtc gacgccgtcg gcagtcggct gctgctcctg accgctgcct
     1561 ccgcgaacgc ggcgcttccc ccgcgacatc cgtcctcgca taaaggcacc tatggacatc
     1621 tcggcatcat cgccggatcg gtcggcaaaa ccggagccgc ggccatggca gccatggccg
     1681 cccttcgaat cggaaccggt ctcgtcacgg ctgccgttcc ctcgagcgtc aatgacatcc
     1741 tcgaggcgaa attactcgaa gccatgaccc tgccgatgcc ggagaccaag gcccgcacct
     1801 ttgcccgttc aggactggat cgcctcctgg cattcgcggg tcctcgcgat gccgtcgcga
     1861 tcggacctgg actgacgacg catcccgaga cggtggactt ggtacaagaa ttcgtgaagc
     1921 gcatcgataa gccgtgcgtg ctcgatgccg atgcgcttaa tgccttggcc ggaaaggcgt
     1981 ccttgctcac ggaatgccct cgcccgccga ttattacgcc ccatcccggt gaaatggcga
     2041 ggcttgaggc agaagccacc acccagtccg tcaatgaaga tcgcctcggg acagcaacgc
     2101 gcttcgcacg tgaacgaggc gtgttcgtcg ttctcaaagg agcccggacc gtcatcgcca
     2161 gaccggacgg ggtcgcagcg atctgcccaa ccggtaaccc aggcatggcc acggcgggaa
     2221 ccggagatgt gttgaccggc atggtcggcg ggttgctcgc ccaaggtgtg gctccatggg
     2281 acgccgcctg tgccgccacc tattttcatg gccttgccgg agatctcgcc gcgcagcaac
     2341 tcggccaggc cggcatgatt gcccgcgatc tgattcgcca tatcccccat gccatcgcgc
     2401 caaccacgca cctctaagct gttgaccact tcacctgcag gagggaagaa ggcaaggcct
     2461 cccgctgtca ccaaaccctg gaaaacgctc cttcgctccc ggcagcaaac tcatcggcta
     2521 ggccgatgtc tgggcaccct ccttgaggga ggcgaagttg tggccctgtt cggagaaatg
     2581 ggtactggga aaacgtcact ggttcgaggc atcgccgatg gacttggcgc cgaacccatg
     2641 gctgtgagta gtccgacctt taccttaatt cacgaatacc gtggccgcct tcctctgatc
     2701 catactgatt tataccgcct cagtgccgcc caactcgaga atacgggcct gggtgactac
     2761 gtagacggcc gcgctattct cgctgttgaa tgggccgaac gctggggcgc agggcttccg
     2821 catgatcggc ttgagattta tcttacccat caccccccgt ccacgcgaca ggcggtgctc
     2881 acagccagag gcccgtctgc atcacgcctg ctcctcgccc ttcgctctcg catgtccgtg
     2941 cgtcgtccca ctagagtggt ttcgcaaacc cgtgtacaat gaccctcggc cagggagggc
     3001 ctcgcattga tccgcttgat attggtaggg acggtactgc tgctggcgct gtcgttcttt
     3061 cttcagaatc aagaacagga ggtcactctc cggtatttct tcggccttcg atcggcgtca
     3121 attctcatct ataagccgat cctggcggcc ttcggcgtcg gcctgctggt ctccggcatt
     3181 ctgctttttc ccgcctgggt ccgcgggcga atcgagctca gacgaaaaac caaggccttg
     3241 caggaagcgg aagtcgatct cgaacggctg cgccaggcgc tcgacaaagc cgcccgccgc
     3301 gttggaagct cttctgacat ccctacggag ggagaacccg tcgatgggtg aggcagacgg
     3361 cacgccgttg atgcggcagt accgcgagat caaacaggcc taccgtgatg ctattctgtt
     3421 cttccgggtc ggcgacttct atgaaatgtt tcaggaagat gcggtcgaag cttccaagct
     3481 tctttcgatt gcgctgacct cccgcgataa atccagcgaa actccgatcc cgctctgcgg
     3541 cgtcccctat cacgccgcaa caaattatat cgccaagctc ttgcgcgcag gccgaacggt
     3601 ggccttgtgc gaacaagtcg aagacccgaa acttgccaag ggtctggtca gacgggaggt
     3661 cgtccggctc tatacgcccg gcacactgat cgatagcgaa ttcctcccct catcggaatc
     3721 caatctgttg gcagccgtgg ccatccgctt gcggcctgcg acacacgaac ctacaggctg
     3781 ttcattcggt ctcgcgacgc tagaggtttc cacgggagag tactggcttt gcgagtttca
     3841 tggtccgcaa gcgcagatcg cactcatgga cgaactcgca cggcttgacc cgaaagagtt
     3901 gctgtatgcc gaagggtgca caccgggaga acaatcctgg atggctgaat taatgggacc
     3961 tcgctgttgc gcacaaccgg caacatggtt tgagctggat accggtcgcg caatgctgca
     4021 ggaacacttc cgtgtgcact cgctagactc cttcgggtgc cagcagctca cattgggtat
     4081 tcaagccgga gcggccgtgc tgcgctatgt gagggagaca caaccttcgg cctcactcga
     4141 ccatattcgg aacttgcgag tgagacagcc tcacgacgcc atgcatctgg acagtgtcac
     4201 gatccgaaat ttggaactcg tcaaacccgc aggacgctgg gacgacgcgc cgaatcaccg
     4261 ctcaccgtat acattgttag gtgtcctcga tcgcacggtc acggcaatgg gaagccgatt
     4321 gctccgcgaa tggcttctgc gcccactaat caactgcgcc ccgattgaag cgcgcctcac
     4381 agcggtcgag gaactcaaag agagcatcga gccacgagtt cgattgcgaa cggcgctacg
     4441 caccgtccag gatatttcgc gactctgcag ccgaatctcg ctcggcgtcg cgaatccacg
     4501 cgacttgctg gccttgaaac tctcgttatc ctccctgcct gccattcagg ccgaactcac
     4561 gccgttgcag gccccgcttc tcgccgagct ctcagcatcc tgggacaacg cgcaggactt
     4621 gtatgaactg attgaacaag ccattcttgc cgacgctcca gtttctatac gcgacggggg
     4681 cattttgaag gacgggtatc acccggatgt ggacgagctt cgcaaagcca ctcgtgaagg
     4741 gaaaggttgg attgcaggac tggaggccaa ggaacgggag cggaccggcg ccgagtctct
     4801 gaaaattcgc tacaaccagg tcttcggata ctatatcgaa ttgacgaaag ccaatctcgg
     4861 caaggttccg tctgactata ttcgtaagca gacactggtg aatgccgagc gatttatgac
     4921 ggctgagcta aaggaactgg aggaacgggt caccggtgcc gatgccaaac tcgtggctct
     4981 cgagcaatcg cttttcgaac agctcagaac aggcctcgcc gccgaaacag cccgcctgca
     5041 agagatcagt cgcagactgg cggttctgga tgtcctcaca gcgttggcgg aaacggctgg
     5101 gttgaatcgt tacgtgcgac ccaccatcga cacgggcgat gtactccaca tcaagcaagg
     5161 ccgtcacccc gtcgtcgaac gacttgatct ctccggagga tttatcccca acgacacgca
     5221 cctggatctg cacaccagcc gccttcacat tttgacaggc cccaatatgg cggggaaaag
     5281 cacctacctc aggcaggtgg cactcattgc gttaatggca cagatcggca gtttcgtccc
     5341 tgcagccgag gccagaatag ggttggtcga tcgcatcttt acccgggtcg gggcatcgga
     5401 caatctcgcg gggggccaaa gtacctttat ggtggagatg accgagtctg ctcatatcct
     5461 tcactgtgcg accgcacgca gcttgatttt gctggatgaa atcggccgcg ggacaagcac
     5521 ctacgacggg ttgagcattg cctgggccat tgcggagttc atccaggatc cgcagcgact
     5581 cggagctcgc acgctcttcg caactcacta tcatgaaatg actcagctgg agggattacg
     5641 agcaggaatc acgaattatt ctgtggcggt gaaggagcgc gacgggaaag tgttatttct
     5701 gcgcaagatc gtccaaggag gcgctgatcg tagttacggt atccatgttg cccagctggc
     5761 gggccttccg gacgcggtca tacagcgagc caaggctgta ctagcacaac tggaatccac
     5821 aacagcacca cgtcccattc ttgaaagtca gcaagctcta cagtttgatt ccactctccc
     5881 tgccccgcat cccatgctgg aagaagtccg ccaaatggac ctcttctcca tgacacccct
     5941 cgatgccctc aatcggctgg ctgcactcca acaacggctt ttgagcgagt aacgattccg
     6001 catcgagcgg cttcgacttc agttcactcc ggccaaagaa aaaggcgaca cccctttcgg
     6061 agtgccgcct tcttcatgag cacatagctc gcgagaactt agtgcttgct gccctgattg
     6121 tacttggcgg tccaaggaat cagattggca attggcttgc catcaggcaa caagacatca
     6181 tactgcaggg gcaaattggc tccatccgga gtcttcggcg acgtgttcaa caacgtcttt
     6241 gcggcatggc aagcagcatc cacttccgtc ttgccttcac attccttcaa acgaagggat
     6301 gaaatgctct tcggcttgcc catttcgccg gcgttgtcat tgagcttctt cacggatgtg
     6361 atcacccggt gattctgctc ggtttcctgg gccacaaact ccactaaggt cgtcggggat
     6421 ccaggaggaa cttcctttgc cgcggccggt ccggaatgcg cacgggccat ggacttcagc
     6481 tgttcccaca cgttcttatc cgcaggatag aacttgaggt tctttccagg atggactctc
     6541 tgccaccaca atccgctttc ggcgttgcca ccgaacaccg cgatgttgag ccacacggcc
     6601 tcatcatacg gatccaagat gatcactcgg ccctgcaacg tggtgggctt gctcagatct
     6661 ccccactcaa atctctcctg gaacgcctcg atggccagcg cagtggaggc aaaacttacc
     6721 accaaagctg cgg
//
LOCUS       DAOQEW010000196         6682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4217_length_6682_cov_7.456013, whole genome shotgun sequence.
ACCESSION   DAOQEW010000196 DAOQEW010000000
VERSION     DAOQEW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4217_length_6682_cov_7.456013"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLS35_16290"
     CDS             <1..376
                     /locus_tag="PLS35_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNI20281.1"
                     /translation="TIVALRGRTEEIKRREVDKVLARLAHLSPEDRAAVEALASAIVN
                     KMVHGTMVTLKAEATSSSGAAYVDAARRFFSLEDAPAAYPPAQTEVQEVSEDSSDGNG
                     ANRFSEASLVQHTTKEQSRRVS"
     gene            463..1392
                     /gene="hemC"
                     /locus_tag="PLS35_16295"
     CDS             463..1392
                     /gene="hemC"
                     /locus_tag="PLS35_16295"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNI20282.1"
                     /translation="MSRALILGTRGSKLAVHQSEWVQARLQELVPGLTISLKRIQTSG
                     DKILDVPLAKIGGKGLFVKEIEDALLSGEIDLAVHSMKDVPTALPDGLDILCVPPRED
                     ARDALITRDGCRLDELKPGARIGTSSLRRQSQLLHHRPDFVIEMLRGNLDTRLRKLRE
                     GQFDAIVLAAAGLRRLGWNAEITEYLPVELSLPAIAQGALGIEARRDDSFVRDVLARF
                     EHRPTRITVTAERALLHRLEGGCQVPIAAHALLDGETLKLDALVASVDGKRVIRQHIN
                     GLATEAHQLGTTLAERLLSAGGDVILKEIYGRA"
     gene            1392..2960
                     /gene="cobA"
                     /locus_tag="PLS35_16300"
     CDS             1392..2960
                     /gene="cobA"
                     /locus_tag="PLS35_16300"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNI20283.1"
                     /translation="MVKTTRKGTVYLVGAGPGDPKLLTLRGKECLEQADVVLYDYLAN
                     EALLQHVPQTAERLYVGRRGRGHYRDQSEINRVLIDQARDGKCVVRLKGGDPFVFGRG
                     GEEAEAVAAAGLDYEVVPGVTAAVAAPAYAGIPVTHRTLASTVTVITGHEDPTKERSS
                     IEWTRLATVHGTLVFLMGMTNLPKIVQCLRAEGKDGTTPVAVIRWGTRVSQRTVVGTL
                     DDIVEKAAAAHMEPPTVIVVGEVVRLRPQLNWFEQRPLFGKRILVTRPKPQAAEFSDL
                     IQAQGGEAVECPTIEIAPPEDQAPLDAALARLSTYNWLIFTSVNGVGPFMTRLLSTQR
                     DVRALAGLTICCIGPRTAEALAAYGLRADVIPEQFQAEGILDSLAGRHLRGAKVLIPR
                     ALVARELLPEQLRAQGAEVDVVPVYRTILPTVALDHLVGQLGAGEIDVISFTSSSTVR
                     NFVGLFPSKDELLRLIGETTIACIGPITAATAREAGLTVHVMAAENTIPALAEAIVRH
                     VEHQRDRRPASANR"
     gene            3013..3405
                     /locus_tag="PLS35_16305"
     CDS             3013..3405
                     /locus_tag="PLS35_16305"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI20284.1"
                     /translation="MVPVKSFMVPKDKFITVERDVDVRTAGRVMRDRNIGSLFVTNGK
                     DVIGIITDTDMVRRVVATGADMTKTTVEQIMSAPLVTIEEHKTLLDANDLMAQTHLRH
                     LGVTKDGQLVGMISVRDLVVFLTNLPRK"
     gene            3410..4393
                     /gene="hemB"
                     /locus_tag="PLS35_16310"
     CDS             3410..4393
                     /gene="hemB"
                     /locus_tag="PLS35_16310"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNI20285.1"
                     /translation="MAFPMQRMRRTRETEPLRRMVRETTLSPSDFIYPVFVTEGQGRR
                     EPINSMPGQCRVSIDLLIKEALEVKSLGIPAVILFGIPDRKDERGSSGFDPNGIVQRA
                     IRALKEQVPELVVITDVCIDEYTSHGHCGIVRNGKIVNDETLDCLRAMAKTHAEAGAD
                     MVAPSDMMDGRVAAIRDELDRAGFVDIPIMAYAAKFASCFYAPFRDAANSSPQFGDRQ
                     SYQMDPANKREALREIDLDVEEGADIIMVKPAMPYLDVISAARERTLLPIAAYQVSGE
                     YSMIKAAAQAGWLDERRAMMESLLSIKRAGAEIILTYFAKEAAALLQSRHA"
     gene            4390..5352
                     /locus_tag="PLS35_16315"
     CDS             4390..5352
                     /locus_tag="PLS35_16315"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNI20286.1"
                     /translation="MKISRGLTPSIARPYSVVTIGNFDGHHHGHRALLDRVVSTARRE
                     SGTALVLTFDPHPVKILAPQVNLMFLTTPEEKLARFEAAGIDEVVFLEFTTAFAALSP
                     AQFAKQVLCDGIGTRELFVGEHFAFGKGRAGRIADLLELGAQLGFRVHPTPPVTIDGE
                     VVSSTKIRQMIQAGDLQKAIRFLGRPYGIEGRVIPGAHRGTELGWPTANLRLPEGRVI
                     PPDGVYAARVLWNGRALDSVVYIGTRPTFGAGERLLEVSILDERLELYGETIRVELLG
                     FVREDRVFASAEALSRQIELDVDAARIQLREAALDTVSPLHSQS"
     gene            5455..>6682
                     /gene="tilS"
                     /locus_tag="PLS35_16320"
     CDS             5455..>6682
                     /gene="tilS"
                     /locus_tag="PLS35_16320"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNI20287.1"
                     /translation="MAVSGGPDSVALLSLLHELAPAWNFSLTVVHCNYGLRGAESDGD
                     ASFVAELCRRLKIPCVVKALQIPAREPGRSSSLQARARELRYQLFRDLVTELGIERVA
                     LGHTADDQAETVLLRMLRGAGLRGLGGMPHRRGNLFVRPLLTVTRREILSYLETKSLS
                     FRTDSSNATSVYLRNRVRHELLPVMQSLAPATVPLLARQADIVREDDRLLERLAARRL
                     VRATVNRDSQTLALDRAVLLKEEPALQRRMLRQAMRSLSPLAIGVRGDVVLSLLASLS
                     AGRSGGSWTVGSLVVTSEQGLVRIAVGDPAGPKTESSSQSASGVALQTVTLSSLPCEV
                     PWPPTGALIRISVVTRERGMALLKKPSSTIAIFDADRLLLPVTVRSWRQGDYFFPAGM
                     AGRRKKLQDYFTDAKLG"
BASE COUNT         1256 a         1842 c         2151 g         1433 t
ORIGIN      
        1 gaccatcgtc gccttgcgtg gccggaccga agaaattaaa cggcgcgaag tggataaagt
       61 ccttgcgcgg ttggcgcatt tgtctccgga ggatcgagcc gccgtggaag cactcgcgtc
      121 agccattgtg aataaaatgg tgcatggcac gatggtgacg ttgaaagccg aggccacgtc
      181 ttccagtggc gcggcctatg tcgatgcggc tcgccggttt tttagccttg aggacgcacc
      241 tgctgcgtac cccccggcgc agaccgaggt gcaggaggta tccgaagaca gcagcgatgg
      301 gaacggcgcc aaccgatttt ccgaggcgtc attggtacag catacaacga aggagcaaag
      361 ccggcgagtg agttgacccg catcgccgtg atcccccatg agcagattat tcgttaccgc
      421 cgctccaacc ttgatgacca gccgatccga ttgtgagttc cgatgagtcg cgcgcttatt
      481 ctcgggacac gtggcagcaa gttagccgtg catcaaagcg agtgggttca ggcgcgtctt
      541 caggaactgg tccccggtct caccatctct ctcaagcgca tccaaacctc gggcgacaaa
      601 atccttgatg tgccgctggc caaaatcggc ggcaaggggt tgttcgtcaa agaaatcgaa
      661 gatgcgctct tgagcggcga gatcgatctc gccgtccata gcatgaagga cgttccgacg
      721 gcattacctg acggcttgga cattctctgc gtgcctccgc gtgaagatgc gcgggatgcg
      781 ctgatcacgc gagatgggtg ccggctcgat gagttgaagc ccggtgcccg catcggcacc
      841 agcagtctcc ggcggcagtc ccagttgctg caccatcgtc ctgattttgt cattgagatg
      901 ttgcgcggca atctggacac gcgtctcagg aaattacgcg aaggacagtt tgatgccatc
      961 gtcttggctg ctgcgggttt gcggcggctg gggtggaacg cagagattac agagtatctt
     1021 ccggtggagc tcagcttgcc cgccattgcc caaggggcgc tgggcatcga ggcgcgcagg
     1081 gacgacagct tcgtccgcga tgtgctcgct cgatttgagc atcgtccgac tcgaatcacc
     1141 gtgacagccg aacgggcctt gttgcaccgg ctcgaaggtg gctgccaggt tccgatcgcc
     1201 gcgcacgcgc tgctcgatgg ggagactttg aagctggacg cccttgtcgc cagcgtggat
     1261 ggaaagcggg tgatcaggca gcatatcaac ggcctggcca cagaggccca tcagcttgga
     1321 accactcttg cggagcggct cttgtcggct ggtggagacg tgattctcaa ggagatctac
     1381 ggacgggcgt aatggtcaag acgacgcgca aaggaaccgt ctatttggtg ggcgccggtc
     1441 ccggtgatcc aaaactgctg acattgcgcg gcaaggagtg tctggagcag gccgatgtcg
     1501 tcttgtacga ttacctcgcg aacgaagcgt tgttgcagca cgtgccccag acggccgagc
     1561 gactgtacgt cggtcggcgt ggtcgaggcc actatcgtga tcaatcggag atcaaccgtg
     1621 tgttgattga tcaggcccgt gacggaaaat gtgtggtccg tctgaagggt ggcgatccgt
     1681 ttgtcttcgg gcggggtggc gaagaggccg aggctgtggc cgcggctggt ttggattacg
     1741 aagtagtgcc gggtgtgacc gcagccgtgg cagccccagc ctatgccggg attcccgtca
     1801 cgcatcggac cctcgcatcg accgtgaccg tcatcaccgg ccatgaagac ccgacgaaag
     1861 agcgcagcag catcgaatgg actcgcttgg cgaccgtcca cggtaccctg gtctttctca
     1921 tgggcatgac caatttgccg aagatcgtgc agtgcctgcg ggctgagggc aaggatggca
     1981 cgacgccggt ggccgtgatc cggtggggaa cgcgtgtatc gcagcggacg gttgtaggca
     2041 ccttggatga tattgtggag aaagcggcgg ctgcgcatat ggaaccgccg accgtgatcg
     2101 tggtcggaga ggttgtgcgc ctccggccgc agttaaactg gtttgagcaa cgtccgctat
     2161 ttggaaaacg gattctcgtc acgagaccga agccgcaagc cgccgaattt tccgatctga
     2221 tccaggctca gggcggggag gcggtggaat gccccactat cgaaattgcg ccccccgaag
     2281 accaggcgcc tctggatgca gcccttgcgc gattgtctac ctacaactgg ttaatcttca
     2341 ccagtgtgaa cggcgtaggg ccattcatga cgcgcctgct cagcacgcag cgtgatgtcc
     2401 gtgcattggc cggtctcacc atatgttgca tcgggccgcg taccgcggag gcgctggccg
     2461 cctatggttt gcgcgcggat gtgattccgg aacaatttca ggcggaaggc attctggatt
     2521 cgttggccgg gcgacacctt cgcggcgcca aggtgctgat tcctcgcgcg ctggtggctc
     2581 gggagctgct cccggagcag ttgcgtgcac agggggcgga ggtcgatgtg gtgcccgtct
     2641 atcgcaccat tcttccaacc gtcgcgctgg atcacctggt tggtcagctc ggagccggtg
     2701 agattgatgt gatttcgttt accagttcgt cgacggtacg aaattttgtc ggattgtttc
     2761 cctcgaagga tgaattgttg cgactcatcg gggaaacgac gatcgcctgc atcggtccta
     2821 tcacggcggc caccgcccgg gaagccggcc tgacggtgca tgtgatggcg gcggagaaca
     2881 ccatccctgc gctggcggag gctatcgttc ggcatgttga gcatcagcgt gatcgccggc
     2941 ccgcctcggc gaatcgatga tcgtggaaag ttgatcggca agatgtgcgt tacttaacag
     3001 ggaggatgcc gcatggttcc agtgaaatcg tttatggttc cgaaagacaa gttcatcact
     3061 gtggagcgcg atgtggatgt gcgcacggcg ggccgcgtca tgcgggatcg caacatcggc
     3121 agcttgttcg tcaccaacgg caaagacgtg atcggcatca ttaccgacac ggatatggtt
     3181 cggcgggttg tggcgaccgg ggccgatatg accaaaacga cggtggaaca gatcatgtcg
     3241 gcgccgctcg tgacgatcga agagcataag acgttgttgg acgcgaacga cttgatggct
     3301 caaacgcatc ttcggcatct cggggtgacg aaggacggcc aactggtcgg catgatttcg
     3361 gttcgcgatt tggtggtctt cctgaccaat ttgccaagga aataagcgca tggcctttcc
     3421 gatgcagcgc atgcgtcgaa cgcgggaaac cgagccgctt cggcgaatgg ttcgagagac
     3481 gaccctgagc ccgagtgact tcatctaccc ggtgtttgtc accgaagggc aggggcggcg
     3541 tgaaccgatc aattccatgc ccggacagtg ccgtgtctcc attgatctcc tgatcaaaga
     3601 ggcgctggag gtgaagtcgt tagggattcc tgccgtgatc ctctttggca ttccggatcg
     3661 aaaggacgag cgagggagct cgggattcga tccgaatggc atcgtgcaac gtgccattcg
     3721 cgccctcaaa gagcaggttc ctgagttggt ggtcattacc gacgtctgta tcgacgagta
     3781 tacgagccac ggccattgcg ggatcgtgcg caacgggaaa atcgtcaacg acgaaacgct
     3841 ggattgtctg agggctatgg cgaagacgca tgccgaggcg ggtgccgata tggtcgcccc
     3901 gtcagacatg atggatggac gagtggccgc cattcgtgat gagctggatc gggccggatt
     3961 cgttgacatt ccaattatgg cttacgccgc gaaattcgcc tcctgctttt atgcgccgtt
     4021 tcgcgatgcc gccaactcca gcccgcagtt cggtgaccgg cagtcttatc aaatggatcc
     4081 cgccaacaag cgggaggcgt tacgggagat tgatctggat gtcgaagagg gcgccgatat
     4141 cattatggtc aagccagcca tgccttatct ggacgtcatc agtgccgcgc gtgagcgaac
     4201 gctcctgccc atcgccgcct atcaggtgag cggcgaatac agcatgatca aggccgccgc
     4261 gcaggccggt tggctggatg agcgtcgagc catgatggag tccctactgt cgatcaagcg
     4321 agccggcgcc gagatcatcc tgacctattt tgccaaagaa gcggccgcgc tgcttcagtc
     4381 acggcacgca tgaaaatttc tcgcggtctt accccctcca tcgccaggcc ctattcggtc
     4441 gtgaccatcg gcaatttcga tggtcatcat catggccacc gggcgctttt ggatcgggtg
     4501 gtgtcgacgg cgcggcgcga gtccggcaca gcgctggtgc tcacgtttga tccgcatccg
     4561 gtcaagatcc tcgctcctca ggtcaatctg atgttcctca ccacgccgga agaaaagctg
     4621 gcccgcttcg aagccgccgg catcgatgaa gtggtgtttc tggaatttac gaccgcattt
     4681 gcggcattga gccccgcgca gttcgcgaag caggtcctgt gcgatggaat tggcacgcgg
     4741 gagttgttcg tcggagaaca ttttgctttc gggaaggggc gggcaggtcg cattgcggat
     4801 ctgcttgaac tcggcgcaca gctggggttt cgagtgcatc cgacgccgcc cgtgacgatc
     4861 gacggcgagg tggtgagctc gaccaagatt cgccagatga ttcaagcggg agatctgcag
     4921 aaagccattc gtttcctcgg gagaccctac ggcattgaag ggagagtaat tcccggcgct
     4981 catcgcggga ccgagctggg atggcccacg gccaatcttc gcctgcctga aggacgcgtc
     5041 attccgcccg atggcgtgta tgccgcgagg gtactctgga acggacgcgc cttggactcg
     5101 gtcgtctaca tcggcaccag gcccacgttt ggggctggtg aacggttact ggaggtgtcg
     5161 attctcgatg aacggctgga gctctatggg gaaacgattc gtgttgagct gctggggttt
     5221 gtgcgtgagg atcgtgtatt cgcctctgca gaggcgttgt ccagacagat tgagttggac
     5281 gtggatgcag cccgcatcca gttgcgcgag gcggcgttgg ataccgtctc tcccctccat
     5341 tcccagtcat gagtgatggc aggtccttcc acagcgacgc ctcatccgct tcataataag
     5401 gtcatccgag ccctccgtac gcgcgcatta cttcaaccgg gccaatcagt cttggtggcc
     5461 gtttccggcg ggccggattc cgtggccctg ctctctctcc tccacgaact cgcaccggcg
     5521 tggaattttt cgctgacggt cgtccattgc aactacgggc tgcggggggc cgaatcggac
     5581 ggggatgcct ccttcgtcgc tgagctctgc cggaggctga agattccctg tgtcgtcaag
     5641 gcgctccaaa ttcctgcgcg tgagccgggt cgttcgagtt cattgcaggc gcgtgcccga
     5701 gaactacgct atcagttgtt ccgtgacctg gtcactgagc tgggtatcga gcgtgtggcg
     5761 cttgggcata cggcggatga tcaggctgaa accgtcttac tgcggatgct gcgcggggcc
     5821 ggtttgcgtg gtttgggcgg catgccgcat cgccgaggga atctcttcgt gcgcccgctt
     5881 ctgactgtga cgcggcgtga gatcctgtct tatctggaaa cgaaaagttt gtcttttcgg
     5941 accgattcca gcaatgcgac atccgtctat cttcgtaaca gagtccggca tgaactgctc
     6001 cccgtgatgc agtcactggc tccggcgacg gtcccgttgc tggcgagaca agccgacatt
     6061 gtgcgcgaag acgaccggct attggagcgc ctggctgccc ggcgtctggt ccgggcgacc
     6121 gtcaatcgag attcacagac gctcgccctt gatcgggcgg tccttctgaa agaggagccg
     6181 gccctgcagc gacgtatgct caggcaggcg atgcgcagtc tctcgccatt ggccatcggg
     6241 gtgcgtggag atgtggttct ctcattgtta gcctctttgt ctgcggggcg ctcaggcggt
     6301 tcgtggacgg tgggatccct ggtggtgaca tcggaacagg gcctggtgcg aattgcggtg
     6361 ggggatcccg ctggtccgaa gacagaatcg agctctcaat ctgcctcggg tgtcgctctt
     6421 cagactgtca cgctgtccag cttgccttgt gaggtgccat ggccgccaac gggtgccttg
     6481 atccgcatct cggtggtgac gcgtgagcgg gggatggcgt tgctgaaaaa gccttcttca
     6541 acaatcgcta tttttgatgc tgacaggctg ttattgccgg tgacggttcg gtcttggcgg
     6601 cagggggatt attttttccc cgccgggatg gccggtcgac gaaagaaatt gcaagattac
     6661 ttcacagatg caaaactggg tc
//
LOCUS       DAOQEW010000197         6631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4277_length_6631_cov_6.166210, whole genome shotgun sequence.
ACCESSION   DAOQEW010000197 DAOQEW010000000
VERSION     DAOQEW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_6631_cov_6.166210"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..789)
                     /locus_tag="PLS35_16325"
     CDS             complement(226..789)
                     /locus_tag="PLS35_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20288.1"
                     /translation="MRLYIACGLTHVPRHKFSSYTEFIHQLAQALQASGAEVRYALKD
                     SDPQLAQRPLSDRARLCYAWDREMVEWADAVIGEATYASTGLGIELQIATQKATPIIL
                     CFQRKEENRVAEAEYLNPDNTHHHLQIGEGYVTLMALGLPTIFKVVGYATEDEAVQSI
                     LHATTLITIQQYNRTQSSQSTGDLGRV"
     gene            complement(805..1836)
                     /locus_tag="PLS35_16330"
     CDS             complement(805..1836)
                     /locus_tag="PLS35_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaC family protein"
                     /protein_id="HNI20289.1"
                     /translation="MILPIRLRGKDLKPHKATVAPLIDARTVLTNSPWQFVSLWLSRE
                     GKKNALFYWNQAHEFAQASTGIGLQSAPLLHYYSFMNAAKALLEAKNINFEPYHGATG
                     NSVTSKRIDLKNEVVKIKSNGILPSLAEYLGDSETDKSHTLKQLMFNLPYIHRTYCLT
                     YPRQPELFFPLSDCCYVFDTHSKEAYLRGKLSSEFSEKRHLKRLPATFVPDTADNYIR
                     SAASVIIASSVISTPEDMRSITGLNQHIRKDLNYINAAETLWYAKATVSGSQRLRRSP
                     LTMTLAAMHRLSEICRYRPLQLQKFLTGHGNWLLNEFVTMAPQQFIDGIAAELTGHQF
                     MLPNVRPAS"
     gene            complement(1961..3664)
                     /locus_tag="PLS35_16335"
     CDS             complement(1961..3664)
                     /locus_tag="PLS35_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20290.1"
                     /translation="MASRIRLDLSTQNPDLQDGLQARIHDPLWMLGRQWQFGEFNGAD
                     AGSPAAAQVIVDTAAVTRYQPGPRAGTLPARPYATNNLALETLVEAEPVVDGSRQNWR
                     LAAESGRHLLRLLETARLGPSRAQWLGSAYALAAPTSEQAKQIDAASLSFIRVLTGRT
                     IDGLRFASRIRTLQAANQLNDIFGDAPLNQVPDVDRAKVLGIVTTWLTWVESLFQQGS
                     ASSAWIPERMEYALSVSAKTASSEVVLTAPEYLDGRLDWFSFMTSPGNGLGATESRTT
                     LTEAFLPAPVSFRGMPSARLWEFEDGAVNFASVQAAPQDLARLLLVKFALEYSNDWFL
                     LPLDLAVGTLSQIRALVVTNTFGERFLIPHASEVDGPASPWRMFSLTNDTQKLFFLPP
                     VLGPMLESQPIEDLSLLRDEMANVAWAVERVVESAAGRPLDRHEAYHETLAEQPPSPT
                     ADADGAPLVYRLGTTVPDYWIPLLPVKEGTTLRLKRGVLPAFGEGGIQGLQLPKGRLL
                     EPNRELLLYEEEVPREGARVTRSYQYARWIDGTTHLWIGRRKDPGRGEGSSGLEFDTI
                     I"
     gene            complement(3667..>6631)
                     /locus_tag="PLS35_16340"
     CDS             complement(3667..>6631)
                     /locus_tag="PLS35_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20291.1"
                     /translation="QALRRAEDARGLKAERERREQTYRAALGAIATLAQQAQAAQTQV
                     TQAAQVLAQQQAAKPEGKVTQPAARRYAIQLLEPRDLDDWENRVEQLTQAQVTAVAQA
                     AAAQQAVSAQEGSRLLAERAQAKLLNAADPDSIPAAQQMATGQETLAAEFDRQALAKE
                     GGQRGKVMTDLLVARATLTTRLAAQWNDALKSLPAAAVVDGLALHRRWTASQQRQAPQ
                     TPWDVTTVPFGNATLGFPPVGSPDFTALVDVLKALDDLVDSVGDSVVAESVYQLVQGN
                     PLRSGATLDAIAAGEVPPPELEVIRTPRSGIGLTHRLCALFPATDGTAPSGWPTNAPS
                     ARAMAEPILNAWVATLLPNPAQVRCKADYMNQETGQIYQTVESALTALGLAPLDAVYL
                     AEGNSRAQQAELEQRWRFALQQTRPTAVPADAVIRLQFGRDNGWGAEIVSVTEWCEVA
                     TTVRRLLSNARSLDGRDLSLPESAAESGLDHEEFSGRATRALQALTEAQRLLTGLLPP
                     PPSDELTADLDDVRHGLFGLARFGIPSAVPMDIGGTGTEARSVLLTQSRSVLLEAQRR
                     LSRVAASEQDFARANATPEERRDHDLARFRIIFGQDFLALPRVTATNAAQLNETFAAS
                     LSLQGQDPLAAVTWFQRAAYVRPGATRLNEALLYGETVGSAALRFQVGQLPFQAQDRW
                     VALPPKPDQPFSRGRVSLVAQMSSAQPLRFDQPFAGLLIDEWGETVPSSSETTAVAFH
                     YDQPNNAPPQALLLAVPSDRRATWDLNSLEAVLQETMDLARLRAVAPDSGDELIWVED
                     QLPEGAAPLGDGENWTWIRMKPEPLSGTRAHQSVIAAGMHQHFFQGAKSPLFVNVGDR
                     LFAHIYLDPARMPRQVMLQWHDGNWEHRAYWGENLIPWGTDNTVSRQYMGPLPPPGRW
                     VRLEVPAATVGVEGRIVGGMAFTLFDGMATWDRAGKRAVQPVGSGEQDPSAPALLFTG
                     GTLDFTGVIDPAIGA"
BASE COUNT         1252 a         1885 c         2095 g         1399 t
ORIGIN      
        1 atcttgtaaa agtagcagtg atttttgaaa tgtctgtagg ttggactcgg gtagggagcc
       61 tggggaaatc cataggagtg tagggtttca gctgtcagcg ttattcctac gcggggtctt
      121 tggaggcagg tgaaggcacg gaggagattt gaccaatcag caccggtggc ggggtggcat
      181 cactcccatc cggccctagc cacatcgaga gcgaaaacaa tgctcctaca ccctgccgag
      241 gtcaccagta gattgagatg attgcgtcct attatattgt tggattgtga tcaaggttgt
      301 tgcatgcaaa atactttgta ccgcttcatc ttcagttgca tatccaacca ccttaaatat
      361 ggtgggtagg ccgagggcca ttagtgtcac gtagccttct cctatttgta gatgatgatg
      421 ggtattgtct gggttaaggt actccgcctc cgctacccgg ttctcctctt ttcgttgaaa
      481 gcacaagatg ataggagtag ctttctgggt agcgatctgc aattcaatgc ctaggccggt
      541 agaggcatag gtagcttcgc cgattacagc atcggcccac tcgaccatct cccgatccca
      601 tgcgtaacag aggcgagccc gatcggagag aggcctctgc gccagttgag gatcgctatc
      661 cttaagtgcg tagcgcacct cagcacctga cgcctgcaaa gcttgtgcaa gttgatgaat
      721 aaattccgta tagctcgaga atttgtgtcg aggaacatgt gtgagaccgc aagcgatgta
      781 taaacgcatt aaaaagactc ttgatcagct tgccggccgt acatttggga gcatgaattg
      841 gtggcctgtt aattctgcgg cgataccatc aataaattgt tgtggagcca tagtcacaaa
      901 ctcattcagc aaccagttgc catgtcctgt aagaaacttc tgcagctgca gcggacgata
      961 ccgacaaatt tcgcttaggc gatgcattgc ggctagggtc attgtaaggg gagaccggcg
     1021 gaggcgctgt gagccagaaa cagttgcttt cgcataccaa agagtttcgg ctgcattaat
     1081 gtagttcaga tcttttcgta tgtgttgatt taagcccgtt atgctgcgca tatcctcagg
     1141 tgtcgaaatg actgacgagg caataattac tgatgcagca gaccgaatat agttatcagc
     1201 agtgtcgggg acaaatgttg ctggcagcct tttcaaatga cgcttctcgg aaaactcgga
     1261 agataacttt ccgcggagat atgcttcctt cgagtgggta tcaaagacat aacagcaatc
     1321 actcaggggg aaaaatagct caggctgtct agggtaagtc aggcaatagg tcctatggat
     1381 gtaaggtaag ttaaacatca actgctttag cgtatgggat ttgtccgtct ccgagtcgcc
     1441 aagatattcg gccaatgatg gcaagattcc gttggattta atttttacaa cttcgttttt
     1501 caggtcgatt cgcttagatg taacgctgtt gcctgtagcg ccgtgatatg gttcaaaatt
     1561 gatgttcttt gcttccagta gtgcttttgc cgcgttcatg aatgaatagt agtggagcag
     1621 tggtgcagat tgaaggccta tcccggtaga tgcttgggcg aattcgtgcg cttggttcca
     1681 ataaaagaga gcgttctttt tcccttctcg ggacagccag agtgagacga attgccaagg
     1741 actattcgtc aaaacggttc tggcgtctat gagtggagca acagttgcct tatgaggttt
     1801 caaatccttg cccctaaggc ggatcggtaa aatcataact aggtgctaac actacctttc
     1861 ttcatgatgg attggaaatc atgctgctgc tttgtgcctg cggcattggg aaatcactct
     1921 gagtcgtaat gggtgagctg ccagtcagtc acgaatgtta ttagatgatg gtatcgaact
     1981 ccagcccgct cgagccttca ccccggcctg ggtctttgcg gcggccgatc cagaggtggg
     2041 tcgtgccgtc gatccagcgc gcgtattgat agcttctcgt gacgcgagct ccttcgcgag
     2101 gcacttcttc ctcatacagc agcaactcgc ggttcggttc gagtagacgc cccttcggaa
     2161 gttgcagacc ctgaatgccg ccctcgccga atgccggaag cacgccgcgt ttgagccgca
     2221 gggtggtgcc ctcttttacg gggagcaggg gaatccaata gtccggcacc gtggtgccga
     2281 gccggtagac cagtggtgcg ccgtcagcgt cagcggtggg ggaggggggc tgttccgcca
     2341 gcgtctcatg gtaggcctca tgacggtcga gcggccggcc ggcggcgctt tcgacgacgc
     2401 gttcgaccgc ccatgccaca ttggccattt cgtctcgcag caacgacagg tcttctatcg
     2461 gctgactctc caacatcggg cccaataccg gcggcaggaa gaataacttc tgcgtatcgt
     2521 tcgtgaggct gaacatgcgc cagggcgagg ccggtccatc cacctcgctc gcgtggggga
     2581 tgaggaagcg ttcgccaaat gtgttggtca cgacgagcgc gcggatctgt gagagtgtgc
     2641 cgacggcaag gtcgagcggg agcaggaacc aatcgttgct gtactccagt gcgaacttca
     2701 caagcagcag gcgggccagg tcctgcggcg cggcttggac gcttgcgaag ttcacggcgc
     2761 cgtcttcgaa ttcccacaag cgcgcggagg gcatgccgcg aaaggagacg ggggcgggta
     2821 gaaacgcttc cgtgagggtg gtgcggcttt ccgtggcgcc cagaccattg cctggactgg
     2881 tcatgaatga aaaccagtcc agtcgtccat cgaggtactc gggcgcagtc aacaccactt
     2941 cgctcgaggc cgtcttggca gagacactga gggcgtattc catccgttcc ggaatccagg
     3001 ccgaggaggc gctgccctgt tgaaagagac tttccaccca cgtcagccag gtggtgacga
     3061 tgccgagcac cttggcgcga tccacgtccg gcacctggtt caacggcgcg tccccaaata
     3121 tgtcattcaa ttgattggcc gcctgcaggg tgcgaattcg agaggcgaat cgtagcccat
     3181 caatggtgcg gcctgtcagg acgcggatga acgagaggct cgcagcgtcg atctgcttgg
     3241 cctgctctga ggtaggggcg gccaatgcgt aggcgcttcc caaccactgg gcgcgtgagg
     3301 ggccgagcct ggccgtttcc agcaggcgga gcaggtgccg tccggattca gctgcgaggc
     3361 gccaattctg gcgcgagccg tctacgactg gctccgcttc caccagcgtc tccagcgcca
     3421 ggttgttggt ggcatagggc ctggccggaa gcgtgccggc gcgcggcccc ggttgatagc
     3481 gggtgacggc ggcagtgtcc acgatcacct gcgcggcggc gggtgagccg gcgtcggctc
     3541 cgttgaattc gccgaactgc cattgacggc ccagcatcca gagcgggtcg tggatgcgcg
     3601 cctgcaatcc gtcctgcaag tcggggttct gagtgctgag gtcgagtctg attcgggagg
     3661 ccatgcctat gctcctatcg ctggatcgat cacgccggtg aagtcgaggg tgccgccggt
     3721 aaacagcagg gccggtgccg agggatcctg ttcaccggag cccaccggct gtactgcgcg
     3781 cttgccggcg cgatcccacg tcgccatccc gtcgaacagc gtaaaggcca tgccgccgac
     3841 gatccgtcct tcaacgccca cggttgccgc cggcacttcg agacgaaccc atcggccggg
     3901 cgggggcaac gggcccatgt attgccggct gacggtattg tctgtgcccc agggaatgag
     3961 gttttcgccc caataggcac gatgctccca attgccgtca tgccactgca acatgacttg
     4021 tcgaggcata cgcgcgggat cgaggtagat atgtgcgaac aggcggtccc ccacattcac
     4081 gaacagcggg ctcttggcgc cctggaaaaa atgctggtgc atgccggccg caataaccga
     4141 ttgatgggcg cgcgtgccgg agagcggttc cggtttcatg cgaatccagg tccagttctc
     4201 accgtccccc aagggagcag cgccttccgg cagttggtcc tccacccaga tcaattcgtc
     4261 gccgctgtct ggcgcaacgg cgcggagtct ggccaagtcc atcgtctcct gcaggacggc
     4321 ttccaggctg ttgaggtccc aggttgcacg ccggtcggac ggcacggcga ggagcagcgc
     4381 ctggggaggc gcattgttgg gctgatcata atgaaaggcc actgccgtcg tttcgctgga
     4441 actcggcacc gtctcacccc attcgtcgat caagaggccg gcgaacggtt gatcgaagcg
     4501 aagcggttgt gccgacgaca tctgcgccac gagcgaaacg cgcccgcgtg agaagggctg
     4561 gtcaggtttc ggcggcagag ccacccagcg atcctgggcc tgaaagggaa gttgtcccac
     4621 ctggaatcgg agggccgcgc tcccgacggt ttcgccatag agcagggctt cgttcaggcg
     4681 tgttgcgccg ggcctgacat acgcagcccg ctggaaccac gtgacggcgg cgaggggatc
     4741 ttgcccctgg agcgaaaggc tggcggcgaa cgtctcgttc agttgcgcgg cgttggtcgc
     4801 ggtcacgcga gggagcgcca ggaaatcctg accgaagatg atgcggaatc gcgcgaggtc
     4861 gtgatcgcgc cgctcttccg gcgtggcatt cgccctcgca aagtcctgct ccgatgcggc
     4921 cacgcggctg agtcgccgtt gtgcctcgag gagcaccgac cgtgattgag tcagcagcac
     4981 cgaccgcgct tccgttcccg tgcccccgat gtccatcggc accgcgctcg gtatgccgaa
     5041 acgtgccaga ccgaacagcc cgtggcgcac gtcgtcgagg tcggcagtca gttcatctga
     5101 cggcggcggg gggagaaggc cggtcagcag acgttgtgcc tcggtcaggg cttgcaatgc
     5161 gcgtgtggca cggccggaga actcttcgtg gtcaaggccg gattcagcgg ccgattcggg
     5221 taacgacagg tcgcggccat cgagtgatcg ggcgttgctg agcagccggc gcactgtggt
     5281 tgcgacctcg caccattccg tcacgctgac gatttccgcg ccccagccat tgtcgcggcc
     5341 gaattgcaat cgaatgaccg catccgccgg caccgccgta ggacgggtct gctgcagcgc
     5401 gaaccgccac cgttgttcca gttccgcctg ctgtgcgcgg ctgttgcctt cggcgaggta
     5461 gacggcatcc agcggcgcca atccgagcgc agtcagcgcg ctctcgactg tctgatagat
     5521 ctgtccggtt tcctggttca tgtagtccgc cttacaacgg acctgtgccg ggttcggcag
     5581 gagtgtggcc acccaggcgt tgagaatcgg ttcggccatg gcgcgtgcgg acggtgcgtt
     5641 cgtaggccag ccggagggcg ccgtcccgtc agtcgccgga aacagtgcgc agaggcgatg
     5701 cgtcagtcca atgccgcttc tcggcgtccg gatcacctcc aactccggcg gcggcacttc
     5761 acccgcggcg atggcatcca acgtggcacc ggagcggagc ggattgcctt gaacgagctg
     5821 gtagacactc tcggctacga cgctgtctcc cacggagtcg accagatcgt cgagtgcctt
     5881 caatacgtcc accagcgccg taaagtcggg gctgccgact ggcggaaatc ccaaggtggc
     5941 gttaccgaac ggcacggtgg tgacatccca gggcgtttgc ggtgcctggc gttgctggct
     6001 cgccgtccat cgacgatgca gggccagccc gtcaaccacg gcggcggccg gcagcgactt
     6061 cagggcgtca ttccattgcg cggcgaggcg cgtggtgaga gttgccctgg cgacgaggag
     6121 atcggtcatg accttgccgc gctgtccgcc ttccttcgcg agcgcctggc ggtcaaactc
     6181 ggcggcgagc gtctcttgcc cggttgccat ctgttgcgca gcgggaatcg aatccggatc
     6241 ggcggcgttg agtaacttgg cctgggctcg ttccgcgagc agacgtgagc cttcctgcgc
     6301 gctgacggcc tgttgggcgg cggcggcctg ggccacggcg gtgacctgcg cctgggtcag
     6361 ttgttccacg cgattttccc agtcgtccaa atcccgtggc tccagcaact ggatggcgta
     6421 acggcgcgcg gcaggttggg tgaccttgcc ttccggtttc gccgcctgtt gttgcgccag
     6481 tacctgagcg gcctgcgtca cctgggtctg cgcggcttga gcctgttgcg cgagcgtggc
     6541 aatcgctccc agagcggcac gataggtttg ctcgcgtcgt tcccgttcag ccttcagacc
     6601 acgagcgtct tccgcccggc gcagggcctg g
//
LOCUS       DAOQEW010000198         6607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4297_length_6607_cov_8.396673, whole genome shotgun sequence.
ACCESSION   DAOQEW010000198 DAOQEW010000000
VERSION     DAOQEW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_6607_cov_8.396673"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLS35_16345"
     CDS             <1..666
                     /locus_tag="PLS35_16345"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNI20292.1"
                     /translation="RYHDVRVEEGAVTSLPQTKTLFPDLIGLEGAQVFGHKLAVRYDT
                     RDKQLVTTSGTYVIGAIELNQNLERREPNRWWRTTFDARQFLPHYNDRMVFVARFLAD
                     AVNGKKTPFYERPTLGGETTLRAFGQNRFVDDTLILVNFEERVRFRRQNIFDHELDLE
                     VAPFVDIGRVMGHFSFGKLTNPQVNPGVGFRVISQPNVVGRLDIGYGKDGANVFVGLD
                     YPF"
     gene            740..>1115
                     /locus_tag="PLS35_16350"
     CDS             740..>1115
                     /locus_tag="PLS35_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNI20293.1"
                     /translation="MVLAALVGCLQTGCSLSMLNAAASGELGKMQTLIAQGHDVNVRF
                     PLVGTGPLIVAAGHGHVDMVRVLLDAGADINATDASGWTALHAAAAKGDRATVVLLLE
                     RGAFVPDHHWYLPSPLSIAQTLG"
     assembly_gap    1116..1215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1236..1823)
                     /locus_tag="PLS35_16355"
     CDS             complement(1236..1823)
                     /locus_tag="PLS35_16355"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNI20294.1"
                     /translation="MELVINGIEQYGYLALFALLMLGIAGLPIPDETILTFAGYLVFK
                     DRLALIPTMLVAFLGSVTGITLSYGIGYSLGRYVVSTMGRAFNIRLENLDQVNVWYAR
                     WGKYALLFGYFVPGIRHLTALAAGSSKLPLTVFVPFAYTGGLVWSGTFVAIGYLLGEE
                     WAHTSAAMHRLLMVVAGFTFLTVTLLLLYRKWRSS"
     gene            2561..2698
                     /locus_tag="PLS35_16360"
     CDS             2561..2698
                     /locus_tag="PLS35_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20295.1"
                     /translation="MNKFTMVLFIGLLLAGVLALSLWFGTPHQDPAWLAATDRELAQV
                     D"
     gene            complement(3380..4054)
                     /locus_tag="PLS35_16365"
     CDS             complement(3380..4054)
                     /locus_tag="PLS35_16365"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI20296.1"
                     /translation="MTLIDRTMREGALTSRFARLLVVVLLAALTSLIFLVPAKAAESE
                     LTPTETVSDMINELFSILKEHQGPELFHERRREIERVIRRDVHYEEMAKRSMGPSWVR
                     MDDQARTDYVDLFVHLLRDALTNRMVQPQDEQIIYLSERRESTCAEVRTRLVGSKLDT
                     TLDFRLIHDDGHWLVYDAIMDGTGLVGTYRVQFASIIRDESLAELLNKLKANTLLVKL
                     FDAHGS"
     gene            4968..>5397
                     /locus_tag="PLS35_16370"
     CDS             4968..>5397
                     /locus_tag="PLS35_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20297.1"
                     /translation="MRHLARVLSLSTILVTLCVGMTSCRAHSDGYWTKPERSQALTNE
                     VYSADSAECQMMVESNSLEETPTYKAKLFAHCMQAKGYTWIVETQKSYPVKEAAIQPA
                     LHCSNGRRIVDAFGFQKCVPDGTKDGGMLQEARRAIPAPVL"
     assembly_gap    5398..5497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5498..6164
                     /locus_tag="PLS35_16375"
     CDS             <5498..6164
                     /locus_tag="PLS35_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20298.1"
                     /translation="QGSLPLIVCIGFLGGCSTTYEPHLLLPVTELTRSIDADVEVHPF
                     VASQELMSGHSPYGVRADDYNNKPPSELTQALTKEVISELSASRVFRKVSTYDGAADL
                     ILTGRIDRFYEQDRRKAWTLIPGVTNKAANLFGANPYTSDGEVQVTMTLRKPTGDMVG
                     SYVGRSEFNEDYTPNSEWHPGDRLNRGLAQAVAQIRDKMLADENLPKTRILPPVPDRS
                     SSR"
     gene            6271..>6607
                     /locus_tag="PLS35_16380"
     CDS             6271..>6607
                     /locus_tag="PLS35_16380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNI20299.1"
                     /translation="MRNTSRRTEASSRLHRKCPALAARQFLFAGDDATHRASILPIGR
                     REQFQASLDRGLCARAGVHDLHFHDLRHTAATWLIQAGVEYATVEKLLGHKLPGMGEQ
                     YIHDWKAKLR"
BASE COUNT         1476 a         1729 c         1775 g         1427 t
ORIGIN      
        1 cggtatcacg atgtccgggt cgaggaaggc gccgtcacct ctttacccca gaccaaaacg
       61 ctgttccctg atctgatcgg gctggagggg gcccaggttt tcggtcacaa actcgcggta
      121 cgctacgaca cccgggacaa acagctggtc acgacgagcg ggacctacgt catcggggcc
      181 atcgaactta accagaacct ggaacgccgt gagccaaacc gatggtggcg tacgaccttt
      241 gacgccaggc agttccttcc ccactacaat gaccgtatgg tgttcgttgc ccgcttcctc
      301 gccgatgccg tgaacgggaa gaagactccg ttttatgagc ggcccacgct cggcggtgag
      361 accaccctac gggcattcgg acagaatcgt ttcgtcgatg acaccctcat cctcgtgaac
      421 ttcgaggagc gagtccggtt cagacggcaa aacatttttg atcacgaact ggacctggag
      481 gtcgcgccgt ttgtcgacat tggccgggtg atgggacatt tcagctttgg caaactgacg
      541 aatccgcagg tcaatccagg ggtcggattt cgtgtgatct cacaacccaa cgttgtcggc
      601 agattggaca tcgggtacgg caaagacggg gcgaacgtat ttgtcggctt ggattatcca
      661 ttttgacgct gttgcactgg tggcgatgag tgcatgcctt catacaggcc tcggcaaaca
      721 ccgacgagct aatgtgtgta tggtcttggc cgccctggtg gggtgtttac agacggggtg
      781 ctccttgtcc atgctgaatg cggcggcatc gggtgaactt gggaagatgc agacgttgat
      841 cgcgcaagga cacgatgtaa acgtccgttt cccattggtc ggcacaggtc cgctgatcgt
      901 cgcggccgga cacggtcatg tcgacatggt gcgtgtattg ttggatgccg gtgcggacat
      961 caatgcgacg gatgccagcg gttggacagc gttgcatgcg gctgccgcga aaggcgatcg
     1021 ggcaacagtc gtcttattgc tggagcgcgg ggccttcgtg cccgaccatc attggtacct
     1081 gccaagtccc ctcagcatcg cgcagaccct tggttnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnncagcg agccgtcggc tgcggtcagg acgaccgcca ttttcgatac
     1261 agaagaagca gtgtgaccgt aagaaaggtg aatccagcca ccaccatgag cagtcggtgc
     1321 atggcggcag acgtgtgcgc ccattcttct cccaagagat atccaattgc gacaaacgtt
     1381 ccggaccata ctaagccgcc tgtataggcg aagggcacga atacagttaa ggggagtttc
     1441 gaagaacctg ccgccagtgc ggtgagatgt cgtatgcccg gcacaaaata accgaagagc
     1501 agggcgtatt taccccaccg ggcgtaccaa acgttcacct gatccaagtt ctcaagacga
     1561 atattgaagg cccgtcccat cgtagagacg acataccgcc cgagactgta gccgattcca
     1621 taactgagcg tgatgcccgt gacactcccg agaaatgcga caagcatggt ggggatgaga
     1681 gcgagtctgt ccttgaacac gagatatccg gcaaaagtga gaatggtttc gtcgggtatc
     1741 ggcagtccgg caatgcctag catgagcaag gcgaacaatg cgagatagcc gtactgctca
     1801 atcccgttga tgaccagttc catgagtgca gtccacgtgt tggctaagta gacgccgacg
     1861 agtacgatct acgacaatgt cgtcatctcc tcatcaatgt cacagaatcg cctctatgac
     1921 caatcattgc tgccattgta ggccctagga cttctaccgg taggcagcgc tcaggtgtcc
     1981 acgctatcat cctgagcgac ctcatttgaa atccctttgc atatgctacg ctgcggcgat
     2041 ggcaagacac agccgtctgg tgaacaatga aacgcgagga accgatctac cactgtccca
     2101 cctgtcgcat ccctgtgatt ccagaacgtc tcttgatccg gccagacttt cctacgcgac
     2161 acgacaggcc caagcggccg gaatggcact gtaatcaatg tgggcggacc tttgcaccgg
     2221 attacttcga cgaactgggg tagcgcgatg ttctagtcat cccataattg ggcggtactt
     2281 gaaaaagcct ttggctgaag tgatgcaccg tctcgcacgg aatacggata aacaagacgt
     2341 tcaacggcaa tggcagcgag ctaggagtag aacaatcacg aactcagaga gccggtaacc
     2401 agcgcccaca cccttttgga tgccggggaa ctcctcgcgt cccgtacgac acacacagtg
     2461 ccctcccact gtaggtcttc gaccacccct ctatggaata tgcctgcgtt ggtcagccac
     2521 cctaaagtgg cggcatgcat gcacttaaac ggaggggcgg atgaacaaat tcacgatggt
     2581 gttgttcata gggttactgc tggccggtgt tctggcattg tcgctgtggt ttggtacgcc
     2641 gcatcaagat ccagcgtggc tagccgccac agaccgggag ttggctcaag tcgattgatg
     2701 ggcttgggat gtgaggaggg cctgcaatca ctcaggccct tgtacagcta gattcatgcc
     2761 tggtgggatt gggctcggat ccccagtctt ttcagttgct tggtaatgtc tgggatagtg
     2821 cgcgtgtcga tgcaattggg cctcggacat tgcgattctg tgactgcaaa ggcggcatac
     2881 ataagcccct gtcagataca caccgcctac actctccaat tgataatcgt gctcgcattg
     2941 tgagtcgatc aatcccatgg caaggagttc tgccacctca tccgcggcct ctttggtagg
     3001 taacggatag tcccattcac ctgtcacagg gtggcctttc tcttccgtct cggcggagcg
     3061 tatgaccaag gcgtgtggga cccattggcc cccgctccgg taagacatca agcgtatggt
     3121 gcaatccttg tacacaacat tggattcgtt gctggatgcg tgattcattg ttccaacctt
     3181 caatccatcg tcaatagtga aaatccaagc ggctcaggag tgctgtcaca ctgttcgcct
     3241 tcagaggaaa ctgggagggc cctagctgac tatacgaact tcagggcggc ggacctgctg
     3301 tcgggatagc tcaactgtaa cggttgcacc agatctcatg cccggttttg gtgcaggggg
     3361 cgacaaatgc atggttcagt catgatccgt gtgcatcaaa cagttttacc agcagtgtgt
     3421 ttgccttgag tttattcagc aattctgcca atgactcgtc gcgaatgatg ctcgcaaact
     3481 gtacacgata ggttccgaca agaccggtcc cgtccatgat ggcgtcgtac accagccagt
     3541 gtccgtcgtc atgtataagt cgaaagtcga gagtcgtgtc cagcttgctg ccaacaagtc
     3601 gtgtcctaac ctcggcacat gtcgactcac gtctctcgga gaggtagatg atttgttcgt
     3661 cttgaggctg aaccatccta tttgtcagcg catcccggag tagatggaca aagaggtcca
     3721 catagtctgt gcgggcttga tcgtccatcc gcacccatga aggtcccatc gaacgcttgg
     3781 ccatctcctc gtaatggacg tcgcgtcgaa tcactcgttc aatttcacgc cggcgttcgt
     3841 ggaaaagctc agggccttga tgctctttca atatggaaaa cagttcattg atcatatcgg
     3901 agacagtttc agtcggcgtc agctccgact ccgctgcctt agcaggtaca aggaagatta
     3961 ggctggtcag tgctgcgagt agcaccacta ccaggagacg ggcaaagcgg gaggtaagcg
     4021 ctccttccct cattgttcga tcgatgagcg tcatcccacg gttcatttca actcaccagc
     4081 cacgatccgg tcctacgcta tttgcagatc agacttcttt ttgagcgaag agaagcagta
     4141 gcacgcgtaa cacatacatg atgctgagga cggaaaagcc gccatatgca aggtagatca
     4201 acaagctcat ccattcgtcc atgaagcccc cgaatgcccg tccttgtcat ttctattaga
     4261 tcgggtgaaa agatacagga attttcgccc cggtactgct aggaagaccc cttgcggagg
     4321 atatcgctat cgggtgacgc gtcgaacgct acaggagatg caggagatga atgataatcg
     4381 aaggggctga agggaagatt attgctatgc cgcgcgacga aagtcagcgg tttcagcatt
     4441 ttttctgttc tccgtatcgt catcgagtgt agccgccgca ttaggtttcg tgcagcggcg
     4501 tatcatctgg gtgatgacga ttgccaggag aatgtaattc aggatgccca ggaccagact
     4561 tatacagatg acctcttcag aaatcaccga agaagtgtag cgcaaggaga gcgatccgca
     4621 agtcgccagg cagagagccg ccataggaaa agactatgcc acttgcgctg gcgcagtttg
     4681 tgtccgttca gtgcgagtgc gatttattct ttgtcaatga tagcccgccg ctccaatcct
     4741 accgcctcga gtatgcgact cgagcgaagt gtaggagaag gtgaagatgt agggaggtca
     4801 gcaagaacca gcgcagcagt ccactggcgc gggcagttca tctcccgtgg gacttaattc
     4861 tcgtcacgtg aagtctatct cagcgtttat ctcagcgggt ttccagccag ggccttacct
     4921 agcgtgcgat cggctctatg aactctatag tgaagggtgg aggtcgcatg cgccatttag
     4981 cacgggttct ctcactcagt acgatccttg ttaccctgtg tgtgggtatg acctcttgcc
     5041 gagcccactc agacggttac tggaccaaac ctgaacggtc gcaggctttg accaacgagg
     5101 tctattcagc agattcagca gaatgccaga tgatggttga aagcaacagc ctcgaagaga
     5161 caccaacgta taaagcaaag ctcttcgccc actgcatgca ggccaaaggg tatacgtgga
     5221 tcgtagagac acagaagtcg tatcccgtaa aagaagccgc catacaaccc gctcttcact
     5281 gctctaacgg gcgacgtatc gtcgatgcat ttggtttcca gaaatgcgta cctgacggga
     5341 ccaaagacgg aggcatgttg caagaggcga gacgtgcgat tcctgctcct gtgctttnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntca aggttcatta ccgttgatcg
     5521 tttgtattgg gttcttagga ggctgttcca caacctatga accgcacctg ctccttccgg
     5581 tgaccgaact cactcgttcc attgatgccg atgttgaggt gcatccgttt gtcgcgtccc
     5641 aggagcttat gtcagggcat tctccgtacg gggtgcgggc cgacgattac aataacaagc
     5701 ccccctctga attgactcag gcccttacca aggaagtgat ctccgaactg tcggccagtc
     5761 gtgtgtttcg taaggtgagt acctatgacg gtgcagccga cctcatactg actggacgca
     5821 tcgaccgatt ttacgaacaa gaccgccgca aagcgtggac ccttattcct ggggtgacga
     5881 acaaggcggc caatttattt ggtgctaacc cgtacacctc cgatggagag gtccaggtta
     5941 ccatgacact gcgcaagccg acaggggaca tggtgggctc ttatgtaggg cgttctgaat
     6001 tcaacgaaga ctacacaccg aatagcgaat ggcatccagg tgaccgactc aaccgaggat
     6061 tggctcaagc tgtggcgcag attcgcgaca agatgctggc cgatgagaac ctgcctaaaa
     6121 caaggattct tcctcctgtc cctgatcgtt cctcctctcg ataaacatcg aagactgggt
     6181 ctcggctcac gaagagcaca gagtgagctg cgcgctcacc cttagccacg gaaccccgag
     6241 aagctaatgc tgtttcgaaa cagacgacaa atgaggaaca catcccgacg cacggaagcc
     6301 agctcaaggt tgcaccgaaa atgtcccgct ctcgccgctc gccaatttct ttttgcaggg
     6361 gacgacgcca cgcatcgggc cagtatcctc ccaataggcc gacgcgaaca gtttcaagca
     6421 tcgctggatc gaggcctgtg tgcgcgcgcg ggcgtgcatg acctgcactt ccacgatctc
     6481 cggcatacgg cggcgacgtg gttgattcag gcgggtgtcg agtatgcgac ggtcgagaag
     6541 ctgctcgggc ataagctgcc cggcatgggg gagcagtaca ttcacgattg gaaagccaag
     6601 ctgcgcg
//
LOCUS       DAOQEW010000199         6576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4326_length_6576_cov_8.781475, whole genome shotgun sequence.
ACCESSION   DAOQEW010000199 DAOQEW010000000
VERSION     DAOQEW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4326_length_6576_cov_8.781475"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="PLS35_16385"
     CDS             <1..1676
                     /locus_tag="PLS35_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20300.1"
                     /translation="NGGTHTTGSGLNFRAAPIAQRLATNPDASQIFSSQVHGDPYTPT
                     LRAYVGDTMVFRLLHTLMNESMVWTLSGHTFWTERYAADANRKNSIHIGIAERYDLVV
                     PEAGGSRHQAGDYLHFNGRSSKFSEGGWGIIRVLDKEQADLKKLPAGFSIKGDIPKTL
                     PVCPADAPVKQFNVVAMDYAGMKFNAKAPESIEVDFERRILMTNAEAKIYALEEDTAK
                     VAAGAHPMPLTLRVNSGDCVKVHLKNKMKDSKASFSAIGLAFDPKDSMGANVGNNPGD
                     QTVAPGGERDYTYYADPFNGETTSLVWDWGNVMTNPRNGLFGAVVVGPKGSKYRDPKT
                     GADLSNKNAWAADVIIDHSVPGNELRANYRDVALFFQDEDNIIGTSFMPYVQNVAGLT
                     GVNYRSEPYKFREEQGCSLGKVFQPCKADKPEDPATPLIEAHAGDPVRIHVLGVSNEQ
                     NGMFSVEKHEWPIEPFMRGADLISVVEFSGSETIDAFLPSAGGMYRMPGDYVYSNQRL
                     PYSQSGQWGYVRVLPAGDTRLLPLTGAGAGAKSAEVEQPAAHAMPVASK"
     gene            1830..2738
                     /locus_tag="PLS35_16390"
     CDS             1830..2738
                     /locus_tag="PLS35_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNI20301.1"
                     /translation="MRTAGIALLAALSIFSSQSWAYEEITVTDGGNITGTVTMIGGKP
                     TPKGYNLITFPDPVYCGRISTGTGWRILDEFSMTSGSGLKDVVVLLTDATKGKPFKFE
                     PVTIEARDCRFLPFVTVVKDGSEVTVMNMDPVMHDIQAYETSQLGPRVLFNTPLPMNP
                     HHKRFVTAESHEHLAGEPVKEAIHMTKGRRIFVMQCGFHAYMESWGLAVDNPYYALTT
                     DGGTFTLGDVPPGEYTLMAWHPALGTMLQKKVTVTEKGTSTVDFAFEAPKGRRSVHEI
                     EHNPHYGPESLGKVVDIRPTLERQVP"
     gene            2766..3698
                     /locus_tag="PLS35_16395"
     CDS             2766..3698
                     /locus_tag="PLS35_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNI20302.1"
                     /translation="MMLNRTQRIHSFAAAVLVWLGVALSPVGAYEVIDVQHGGTVDGT
                     VTLSGGIPDPKAFNLITFPDPTYCGRISNGSGWRLLRDFIVSPEGGLKNAIVTLEGVE
                     SGKAFDMSVPLIEARDCMFQPWVTIVRNGHAVEVVNMDPVMHDIQGYETSPEAGARVL
                     FNTPLILNHQHQRGNMRAVHNHAPGKSLVGPVYLNKGRRTFYMQCGFHAYMESWAMAV
                     NNPYYAVTDDQGAFKIENVPPGIYQMVVWHPQSGPGVTRTITIAPDGTTTEKVALPAP
                     KGVRTAYRAMENPRFGLESLGHPVEIQPLVEHQH"
     gene            3880..4344
                     /locus_tag="PLS35_16400"
     CDS             3880..4344
                     /locus_tag="PLS35_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20303.1"
                     /translation="MFAKSDRIRLEYKYAIKTELGYSSIEILRLDKRESWFLLAQRRQ
                     ILSLPIKPEEILPIQPTLPGEQRRTLIGDATTIGRASRLYEVRVDYNGRNERFYEWVD
                     VETGIVLKLVSQDRDWSIEYLRFRLSPQPDYYFEEPTGYQRWVPKPNAQERG"
     gene            4375..5772
                     /locus_tag="PLS35_16405"
     CDS             4375..5772
                     /locus_tag="PLS35_16405"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNI20304.1"
                     /translation="MGLQTKRRWIRTVNLCLAAVIVSGPLVGLWPVPANAGSLKDAQA
                     AFDKKQYQEALDIIEQVTKEKGSQPETRRLKVRSLIFLGKPKDALVEYERHEQESKQE
                     DRPLLKDVSLGFIYALVKDMREQMRGAAYTALKDVDSAETIPALEDGLSDGSGLVRAL
                     AAEALGKLDAGRKSPRLRNALEDQAGLVKATVIKVLGKSGDRSVIPLLEKALKDEQPA
                     VRLAAAGALYHTGQTAMWETIQKAAAAPNPEERATALRMVGDLKDARGLSILLEAMTN
                     TQPSVRGAAASALGELGKVQGIPALEKALDDKIPAVKTSAAISLGELGVKESLVALRK
                     SLADPNPVVKAAVVSALLRVEEPFESVAEALYELAQNNDPGTRSAAGKAAGRAHGANT
                     QPAIEFLAGLLKDSIPRPRIAAARALGQIGGAEVLPILKAALHDEDDAVRATVGGSIA
                     RIIGHAKFPSNQAKS"
     gene            5975..>6576
                     /locus_tag="PLS35_16410"
     CDS             5975..>6576
                     /locus_tag="PLS35_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20305.1"
                     /translation="MKKGAMKAVFGVAAAAFLAAPLTAFAGGTIAGKVTYPGKAEQKE
                     FSFAKFPNPKFCPKNPNKSLMDGDKRFLKTIEVAKDGGLKGAVVAVVDIEDQAFQDGF
                     KGTDVVAEFCEFLPFSGVVVNNKNFRAENKDADPDDPKSVQGVLHNPHSFTVKGSTSA
                     TGFNIGLAKKGDKLEKPVTFRGGAEKAGYYRLQCDQHEFM"
BASE COUNT         1435 a         1718 c         2067 g         1356 t
ORIGIN      
        1 tgaatggtgg cacgcacacc actggcagcg gattgaactt ccgggcggcg ccgatcgcgc
       61 agcgattggc gaccaatccg gatgcgtcac agatcttcag cagccaggtg cacggcgatc
      121 cgtacacgcc caccttgcgc gcctatgtgg gtgacacgat ggtgttccgt ctgttgcata
      181 ccctcatgaa cgagtcgatg gtctggaccc tgtccggcca cacgttctgg accgagcggt
      241 atgccgcgga tgcgaaccgg aagaattcga ttcatatcgg tatcgcggag cggtatgacc
      301 ttgtggtgcc ggaagcaggt ggatctcggc atcaagccgg tgattacctt cacttcaacg
      361 gccggtcctc gaagttctcc gagggtggct ggggcatcat ccgggtcttg gataaagagc
      421 aggctgattt gaagaagctg cccgcgggct tctccatcaa gggcgacatt ccgaagacct
      481 tgccggtctg tccggctgac gctcctgtga agcagttcaa tgtggtggcg atggactatg
      541 ccggcatgaa gttcaatgcc aaggcaccgg aatccatcga agtggacttt gaacgacgga
      601 tcctcatgac caatgcggaa gccaagatct acgcattgga agaggacact gccaaggtcg
      661 ccgccggcgc gcatccgatg ccgctgacgc ttcgcgtgaa cagcggtgac tgcgtcaagg
      721 tgcatttgaa gaacaagatg aaggacagca aagcctcctt ctctgcaatc ggcctggcct
      781 tcgatccgaa ggattccatg ggtgcgaacg tagggaacaa tccgggagac cagaccgtgg
      841 ctccgggagg cgagcgtgac tacacgtatt acgcggatcc gtttaacggc gaaaccacct
      901 cgctggtgtg ggattggggc aatgtgatga ccaatccgcg caacggattg ttcggtgccg
      961 tcgtcgtcgg tcccaaggga tccaagtatc gtgatcccaa gaccggcgcg gatctgtcga
     1021 acaagaatgc ctgggcagcc gatgtcatca tcgaccattc ggtgccgggc aatgaactgc
     1081 gggcgaatta tcgtgacgtg gcgttgttct tccaggacga agacaacatc atcggcacga
     1141 gcttcatgcc ctatgtgcag aacgtggcgg gtttgaccgg cgtcaattac cggtcggaac
     1201 catacaagtt ccgtgaagag cagggttgtt cgctgggcaa ggtcttccag ccttgcaagg
     1261 cggataagcc ggaagatccg gccaccccac tcatcgaggc gcatgcggga gatccggtgc
     1321 gtatccatgt gctgggtgtc agcaatgagc aaaacggcat gttcagcgtg gagaagcacg
     1381 aatggccgat cgagccgttc atgcgtggcg ctgacctgat cagtgtcgtg gaattctccg
     1441 gttccgagac gatcgatgcc ttcctgccct cagcgggtgg aatgtatcgg atgcccggcg
     1501 actatgtgta tagcaaccag cggttgccat actcgcagtc cggtcagtgg ggctatgtgc
     1561 gggtgttgcc agcaggcgat accagactgt tgcctttgac cggcgccggt gcgggagcca
     1621 agagtgccga agtcgagcag cctgctgcgc acgcgatgcc tgtcgcgtcg aagtaagcag
     1681 gtcaatctca cgcgaggtga gaaagggggg agcctgaaag ggctcccccc ttttgttatg
     1741 agatggggat tgtgtatgat cagctcagta tccgagatca tttcgactga gactttctga
     1801 ccaacccatc atcgttggag gattgataca tgcgaacggc cggaattgct ttgcttgctg
     1861 ctctcagcat tttctcttct caatcatggg cttacgaaga aataaccgtc acggacggtg
     1921 gcaatatcac gggcacggtg accatgatcg gggggaaacc gaccccgaaa ggatataacc
     1981 tcattacctt tccggatccc gtgtactgcg gccgtatctc gaccggaacg ggctggagaa
     2041 ttctcgatga gttctcaatg acatccggaa gcggtttgaa ggacgtggtg gtcctcttga
     2101 ctgatgccac caagggcaag ccgttcaaat tcgaaccggt gacgattgag gcgcgtgact
     2161 gccggtttct cccgtttgtc acggtcgtca aagatggctc cgaggtgacg gtcatgaaca
     2221 tggacccggt catgcatgac attcaggcct atgagacgtc tcagctcggg ccgcgtgtgc
     2281 tgtttaatac gcctctgccc atgaatcccc atcacaagcg ctttgtcacc gcggaaagcc
     2341 atgagcatct ggcgggcgag ccggtgaaag aagccattca catgaccaag ggacggcgga
     2401 ttttcgtgat gcagtgcgga ttccacgcct acatggaaag ttggggcctg gccgtcgaca
     2461 acccctatta cgcgctgacg actgatggcg gcacgttcac cctcggtgat gtgcctccgg
     2521 gcgaatacac gctgatggcc tggcatccgg ccttgggaac gatgctgcag aagaaggtga
     2581 cggtgacgga gaagggaacg tccacggtcg atttcgcgtt cgaggccccg aaaggccggc
     2641 gcagcgtcca cgagatcgag cataatcccc attatggacc ggaatccctt ggcaaggtcg
     2701 tggatattcg cccgacgtta gaaaggcagg ttccgtagca gcgcgaattg cagacaagga
     2761 aggtcatgat gctgaatcga actcagagga ttcattcctt cgcagcggct gtgttggtgt
     2821 ggctgggggt ggccctgtcg ccggtagggg cctatgaggt gatcgatgtg caacacggcg
     2881 gcaccgtcga tgggacagtg acgctgtctg gcgggattcc agaccccaaa gccttcaatc
     2941 tcatcacctt tccggacccg acgtactgtg ggcggatttc taacggaagt ggatggcgtt
     3001 tgctgcgcga ttttattgtc tcccccgagg gaggactgaa gaacgccatt gtcacgctgg
     3061 aaggagtgga atcggggaaa gcattcgaca tgtccgtccc cttgatcgaa gcacgcgatt
     3121 gcatgttcca accatgggtc acgatcgtcc ggaacgggca cgcggtggaa gtcgtgaata
     3181 tggatcccgt catgcatgac atccagggct atgaaacctc gccggaggct ggagcgcggg
     3241 tcttgttcaa tacgccgttg attctcaatc atcagcatca gcgcgggaat atgcgcgccg
     3301 tgcacaatca tgctcctggc aagtccttgg tggggccggt ctacttaaac aaagggcgac
     3361 gaacgtttta catgcagtgc gggtttcacg cctatatgga aagctgggcc atggccgtca
     3421 ataatcccta ctatgcagtc acagacgacc agggggcctt caagattgag aatgtgccgc
     3481 cgggaatcta tcaaatggtc gtgtggcatc cgcagtcagg tcctggggtg acgcggacga
     3541 tcacgatcgc tcccgatggc acgacgactg aaaaggtagc gcttcccgca ccaaagggcg
     3601 tccgcacggc gtaccgcgca atggagaatc cgcgatttgg gcttgaatcc cttgggcatc
     3661 ccgtggagat tcaaccgttg gtggagcatc agcactaatc gcggccgggc cggcatcgtg
     3721 ccatggcttt cgagagggtg cctctccgtt agcctctgtc ttctgtcgat cgtgcctgct
     3781 tggggtgaag attcctctag cgtaccgtcg gtcacacagt ccgttgaatt ctcggcatcg
     3841 ctcgtaaagc gggtggaggg tcgtcggttc gaagcccagg tgtttgccaa gagtgatcgc
     3901 attcggttgg agtacaaata tgcgatcaag accgaactgg gttattccag tatagaaatt
     3961 cttcgtttgg ataaacgcga gtcgtggttt ctgctggccc agcgccggca aatcctctcg
     4021 ctccctatca aacctgaaga aatcctgccg attcagccca ccctaccggg agaacaacgc
     4081 cggacgttga tcggcgatgc caccaccatc gggcgagcct ccaggctgta cgaagttcgg
     4141 gtggattaca acggccggaa tgaacggttc tatgaatggg tcgatgtgga gacgggcatc
     4201 gtgttgaaat tggtgagcca ggatcgtgat tggtcgatcg aatatctccg ctttcggctt
     4261 tctcctcaac cggactatta tttcgaagag cctaccgggt accaacgctg ggttccgaaa
     4321 ccgaatgcgc aagagcgagg atgaacgtga gtggcacgag caggaaggga tttcatggga
     4381 ttgcagacta agaggcgttg gattcggacc gtcaacctgt gcttggccgc cgtcatcgtg
     4441 tccgggccgc tggtcggcct ctggccggtt cctgccaatg ctggttcgtt aaaggacgcg
     4501 caagctgcct tcgacaagaa gcaatatcag gaagccctgg acatcattga gcaagtcacg
     4561 aaagagaaag gcagccagcc ggagacgcgc cggctgaaag ttcggtcgct gatttttctt
     4621 gggaaaccga aggatgccct ggtcgagtat gaacggcacg agcaggagtc aaagcaggag
     4681 gatcggccgc ttttaaaaga tgtgtcgctc gggttcatct atgcactcgt caaagatatg
     4741 cgcgagcaaa tgcgaggcgc ggcgtatacg gcgttgaaag atgtcgattc ggccgaaacg
     4801 atcccggcgc tggaggacgg attgagtgat gggtccggtt tggtgcgtgc cctggccgcc
     4861 gaggctttgg ggaagctgga cgcggggcgg aagtcgccgc gcttgcgcaa tgcgttagag
     4921 gatcaagccg gattggtgaa ggccacggtc atcaaagtgt tggggaaaag cggtgaccgt
     4981 tccgtgattc cgctgcttga aaaagccctc aaagacgaac aaccggctgt gcgcctcgcg
     5041 gccgcagggg ccctttacca taccggccag actgcgatgt gggaaacgat tcagaaggca
     5101 gcggcggctc caaacccgga agagcgtgcc acggccctca gaatggtggg ggatttgaaa
     5161 gatgcgcggg ggctgtcgat tctcctggag gcgatgacca atacccagcc ttccgtgcgg
     5221 ggcgcggcgg cgtcggcatt gggagagctg ggcaaggtgc agggcattcc cgctttggag
     5281 aaggcacttg atgataagat accggcagtc aaaacctcag cggcgatcag tttgggtgaa
     5341 ttaggcgtga aagagtcgct ggtggcgtta cgaaagtcgc tggctgatcc gaaccccgtg
     5401 gttaaggcgg ccgtcgtctc ggctctgttg cgagtagagg agccctttga gtccgtcgcc
     5461 gaagcactgt acgagttggc gcaaaacaac gacccgggaa cgcgttcggc agcagggaaa
     5521 gcggcgggcc gggcgcatgg cgctaatacg caacctgcga tcgagttcct tgccgggttg
     5581 ctgaaagatt ccattccacg accgcgtatc gccgccgctc gagccctcgg gcaaatcgga
     5641 ggggcagagg tcctgccaat attgaaagcg gcattgcatg atgaagatga tgccgtccga
     5701 gcaacggtcg gcggatcgat cgcgcggatc attggccatg cgaaattccc gtctaatcaa
     5761 gcgaaatcat gagaggtcag gccgcaggga tttcaagttg acagtgcgag tgggattcca
     5821 gtatagagaa atgttcagag gcctaaacgc agtccttcca cggctgcgtc atgagcggag
     5881 aacgatccaa caaggggcat tggtcaccag cccttgtggt tgagtggtga cgaagtagtt
     5941 ggccggttaa tgttcattac aaggaggcag tgacatgaag aagggtgcga tgaaagcagt
     6001 attcggcgtt gcggccgcgg catttttggc tgcgcccctc accgcgtttg ccggtggtac
     6061 gatcgccggg aaagtgacct atcccgggaa ggcagagcag aaagagttct ccttcgcgaa
     6121 gttcccgaac ccgaagtttt gccccaagaa cccgaacaag agcctcatgg atggcgataa
     6181 gcgcttcctc aagaccattg aagtcgccaa ggacggcggt ctgaagggtg cggttgtggc
     6241 cgtggtcgat atcgaagatc aggctttcca ggatggtttc aagggcacgg acgtcgtggc
     6301 ggaattctgc gaattcctcc ccttcagcgg cgtcgtggtg aacaacaaga atttccgcgc
     6361 tgagaacaag gacgctgatc ctgatgatcc caagtctgtc cagggtgtgt tgcacaaccc
     6421 gcacagcttc acggtgaagg gctccacctc ggccaccgga ttcaacatcg gtttggccaa
     6481 gaagggtgac aagttggaga agccggtgac tttccgtggc ggcgccgaaa aggctggcta
     6541 ctatcggttg cagtgcgacc agcatgagtt catgca
//
LOCUS       DAOQEW010000200         6511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4397_length_6511_cov_10.425341, whole genome shotgun sequence.
ACCESSION   DAOQEW010000200 DAOQEW010000000
VERSION     DAOQEW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4397_length_6511_cov_10.425341"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLS35_16415"
     CDS             complement(<1..1061)
                     /locus_tag="PLS35_16415"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HNI20306.1"
                     /translation="MLSLRTGLPGHGKTLRTIWEVEQVREKTDRIVYQNGIKDLKLPW
                     HELENPRYWMNVPDGSIIVIDECQRVFPPRAAGSNVPDHVRALETHRHRGIDMYLVTQ
                     HPRLLDSHVRSLVEVWEHLKRPFGQSYVKVTKWQECHGDPTKGLDDAEQSRWRYPKAV
                     FDWYTSAAIHTVRPRAPVGKILLALALLFFVSYAFVRIYSKFHPDPVQPQPQPQQLQH
                     GASSARSTAQVSLPPSSPGPAPATDAAGSSPVLDAQGWAREWVPVIAAVPASAPAYQR
                     AGSTRPAAFPRVVGCVASATACECFTQQGTTVPLDPAQCVAFIQRPPFDPYRADPVVD
                     QQPAVSGSGARAPETRAAAA"
     gene            complement(1068..1340)
                     /locus_tag="PLS35_16420"
     CDS             complement(1068..1340)
                     /locus_tag="PLS35_16420"
                     /inference="COORDINATES: protein motif:HMM:NF022194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HNI20307.1"
                     /translation="MWTAVIAIVGPIIAQILAVLGIGLVTYSGTTVVLDQLKSLVVSS
                     FQGLPADAIHLLGLFGADQYVSIMLSAVAAKVALSSVRNSISIVKK"
     gene            complement(1343..2503)
                     /locus_tag="PLS35_16425"
     CDS             complement(1343..2503)
                     /locus_tag="PLS35_16425"
                     /inference="COORDINATES: protein motif:HMM:NF041109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor TspB C-terminal domain-related
                     protein"
                     /protein_id="HNI20308.1"
                     /translation="MSPFSCTYTYTQSDSPGSSSWTLSITPTYYACPSGQSYDASASS
                     SNYCGTSNSCISGLTPAGSGYPVATPGHSGDGRQITFCASSKCQATAWESISVSFCAD
                     NSSSCPPVAYLRSEMTYTGQSCTTPNLSDKQPFCSGGMQLLGGVCVCPAGARVQGGVC
                     RQLIDCSTGYHRVNDSCISDACPAGEIIQDNKCVPDPLSTVKNPNPATGEPPIACAAG
                     RHYDTSLASCVSNTAANGGTSGSPGTPGNPNTGNGGVGGGLPGESGSAESSDCAQAPV
                     CAGDAVQCAMLTQVWRNHCESVPQSVISAHPVPAIESVDLSASAISSLNAPAALTFAA
                     VCPADKSLSIMGHSYVLSWQPACDGASYLKPIIIAIAFLHGLYVVFSIRKGE"
     gene            complement(2858..3079)
                     /locus_tag="PLS35_16430"
     CDS             complement(2858..3079)
                     /locus_tag="PLS35_16430"
                     /inference="COORDINATES: protein motif:HMM:NF017194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNI20309.1"
                     /translation="MRFRNFVRKYGARVVSIASVPVALVYAAASHAAVDTSVTTALSD
                     AAADTKTVAAASFAVILGVTVFRYFRRAI"
     gene            complement(3351..3692)
                     /locus_tag="PLS35_16435"
     CDS             complement(3351..3692)
                     /locus_tag="PLS35_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20310.1"
                     /translation="MADPAVTFQAPAAAAVSSIPSAAANVTLLEGMVVSVREYGTLFE
                     HSVALPGPDPYTAGGEALLKSKKRFGEGEILKLKCRVTGRKREWERREGRHSEVVRTT
                     DTWLIPFDERH"
     gene            complement(3706..4755)
                     /locus_tag="PLS35_16440"
     CDS             complement(3706..4755)
                     /locus_tag="PLS35_16440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="HNI20311.1"
                     /translation="MLDWLTASLPFSALPEQVWQHLSTIGDRIMRYCPRSNEVKWESP
                     AWDSVRSDSHQIAFRVGSDAIHIQGSPCRVVGDGDAVFGPVDGLRPADGLRAMVRHAC
                     KVLSVILPLDPSLWRVSRMDCTVHLLFDDLPSVRVALAHLRNLEGGRYRVSQQAGDTV
                     YWNHSSRRKAAKAYAKGPHLSYLMKKKDYTGRQYSDSEIALANRLLRLELRLGSQFWN
                     EQNKVSPWFEMSAADVDAAAWVHFDKLLGDASVIHVSDLEASLRSVTVNGESLTDGAV
                     RSALGLWGLIGSFGWERAREMTPRTTWYRNLKYLRLAGLSDLDISSGRIVELRAERLC
                     CARRVTSWSELRAVA"
     gene            complement(5712..6029)
                     /locus_tag="PLS35_16445"
     CDS             complement(5712..6029)
                     /locus_tag="PLS35_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20312.1"
                     /translation="MNRVLSVNRSSLLIFLSVSMFIPIIGLGSVIFRISGWRITVTSW
                     RAVNRKAYCRMNRALSFVNFAKSIGIAAANYRFGEITKRRGLIMQSSVLMNPVIMLRL
                     PGF"
     gene            complement(6271..>6511)
                     /locus_tag="PLS35_16450"
     CDS             complement(6271..>6511)
                     /locus_tag="PLS35_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20313.1"
                     /translation="DFCESCDGFGFTEVDLSEPDPSIAKTLALSLAECVRVIDDFYHC
                     PVSSDVDLHAALKAACQRARATLNETGYSLSRWPF"
BASE COUNT         1837 a         1886 c         1716 g         1072 t
ORIGIN      
        1 gcagccgccg cgcgcgtctc cggcgcccgg gcgccggagc cactcacggc gggctgctgg
       61 tcgacgacag gatcagcgcg atacggatca aacggcggcc gctggatgaa ggcgacgcac
      121 tgcgcaggat caagaggaac ggtagtgccc tgctgggtga aacactcgca agcggtggcg
      181 gacgcgacac agccgacgac ccgaggaaac gcagcggggc gagtcgatcc ggcccgctga
      241 tacgcaggcg cagaagcagg gacagcggcg atgaccggca cccactcacg cgcccaaccc
      301 tgagcatcga gcacgggaga ggaacctgca gcatcagtcg ccggagccgg tcccggcgaa
      361 gagggcggca acgatacctg ggcggttgat ctggcagagg acgcaccgtg ctggagctgc
      421 tgcggctgcg gctgcggctg aacaggatca ggatggaact tggaataaat gcggacaaaa
      481 gcataagaaa caaaaaaaag aagcgctaac gcaagaagaa tctttccgac tggcgcgcga
      541 ggccgcacgg tgtgaatagc ggcagacgta taccaatcaa aaacagcttt tgggtaacgc
      601 caacgactct gttcagcatc gtctaaccct ttagtcggat caccgtgaca ctcttgccac
      661 ttcgtaactt ttacatatga ctggccaaaa ggacgcttta agtgctccca cacttcaaca
      721 agcgaacgaa catgagaatc caataaccgt ggatgttgag taactaaata catatcaatg
      781 ccacgatgac gatgagtttc taatgcacga acgtggtcag gaacattaga gccagcagca
      841 cgcggtggaa aaacacgctg gcattcatca atcacaataa tagagccgtc gggaacattc
      901 atccaataac gcggattctc cagttcatgc caagggagtt tcaaatcttt aataccattt
      961 tgatacacaa tccggtcggt cttttcgcga acctgctcaa cttcccaaat agtgcgcaat
     1021 gttttgccat ggccgggtaa tccagtgcga agagacagca tattccctca ctttttcacg
     1081 atgctgattg aattacgaac cgaacttaaa gcaactttcg cagcaacagc cgaaagcatt
     1141 atcgaaacgt attgatcggc gccgaataaa ccaagcagat gaattgcatc agcgggaagg
     1201 ccttgaaaag aagaaacaac cagagatttc aattgatcca atacaacggt tgtaccggaa
     1261 taagtaacaa gaccaatacc aagaacggcc aatatttgcg caataatcgg tccgacaata
     1321 gcaattacag cagtccacat tactattcac cctttctaat tgaaaaaaca acataaagcc
     1381 catgcaaaaa tgcaatggcg ataataattg gtttcaggta agaagcaccg tcacaagccg
     1441 gctgccaact caaaacatag gaatgaccca taatgcttaa agacttatct gccggacaaa
     1501 cagcagcaaa agttaaagca gcaggagcat tcaaagatga aattgcagaa gcagacaaat
     1561 caactgattc gattgccgga acaggatgag cggatataac agactgagga acagactcac
     1621 aatgattacg ccatacctga gtcagcatcg cacactggac cgcatcgcca gcacatacag
     1681 gagcctgcgc acaatcagaa gattccgcag agccagattc accaggtaaa ccaccaccta
     1741 caccaccgtt accagtatta ggatttcccg gagtgccggg ggagccagaa gtaccgccgt
     1801 tagcggcagt gttgcttaca caactggcaa gactcgtatc gtaatgacga cctgcagcgc
     1861 aagcaattgg tggctcgcct gtagctggat tggggttttt gacggtagat aaaggatcag
     1921 gaacacactt gttatcctga ataatttcac ctgcaggaca tgcatccgaa atacaggaat
     1981 cgttaactcg atgatagcca gtagaacaat caattagctg acggcaaacc ccgccctgaa
     2041 cacgagcacc agcagggcaa acgcaaacac cgccgagcaa ttgcatgccg cctgaacaaa
     2101 acggctgctt gtctgaaaga ttaggggtag tacaagactg gcccgtatac gtcatttcag
     2161 agcgcaaata agcaacaggc ggacacgaag atgagttatc cgcacaaaaa gaaacggaaa
     2221 ttgattccca cgcggttgcc tgacacttgg aagaagcgca aaaagtgatc tgcctaccgt
     2281 caccagaatg acctggagta gcgacaggat atccagagcc ggcaggagta agaccagaaa
     2341 tacaagaatt agaagtgccg cagtaattac tagatgaagc agaggcatca taactctgtc
     2401 cggatggaca agcgtaataa gtgggagtaa tagaaagagt ccaagaagaa gaacctggag
     2461 aatcagattg cgtgtaagta taggtacaag aaaaaggaga catggaagca gagcaattac
     2521 taccaataac cgtctgagat accccactct gagatgtcca attacaaaga gtaccgtcaa
     2581 tacacctcgc actaacggca cctgagccgg cagtctcact aattctgacg gtcttaccat
     2641 ttcggctata agtataataa ccgctagcaa aacaattaac ggaatgtaat ccaagcagaa
     2701 ccgagaatag caagcacaac gtaaatagtc ggatcaatcg taaaggtaag cacatttaat
     2761 ccctcccaaa aagaaaccgg gcttttgccc ggcttctcag tttgaacagc ccaccgcttt
     2821 gcgtagcatt aaacaagcta cagaaagcgc gtcagtctca gatggcgcgg cggaaatagc
     2881 ggaacaccgt gacgccgagg atgaccgcga aggaagccgc ggcaacggtc ttggtatcgg
     2941 ccgcggcatc gctcaatgcc gtggtgacgg acgtatcgac cgccgcatgg gaagccgcgg
     3001 cataaaccag cgcgaccgga accgaggcga tggaaacgac gcgagcaccg tacttgcgaa
     3061 cgaaattgcg gaaacgcatt ttcaaaactc ctatagagaa cggcgaaatt ggcggtatcc
     3121 ccaagatacc gcccacactg caacgacagc acccgacatt tcgagcgcat cgcccatttc
     3181 cgcgtgagaa taagacgaac acgcaggaaa ttcagaaggg caaccctgac tagccacata
     3241 ttcaggcact acggacgcgc ccggacaact caacgtcacg gcagcaacgt caggctgaac
     3301 gacagccgta ataccagacg gacaattaac ggtcagggtt gccacgatca ttaatgccgt
     3361 tcgtcgaacg gaatcaacca ggtatcggtt gtgcgcacga cctcggaatg gcggccttcc
     3421 ctgcgctccc attcacgctt acgcccggtc acccggcact taagcttgag aatttcgccc
     3481 tcgccgaaac gcttcttgga cttcaacaac gcttcaccgc cggcggtata cgggtccgga
     3541 ccaggaagcg cgaccgaatg ttcaaaaagc gtcccgtact cacgcacgga aacaaccatg
     3601 ccttcgagca gagtgacatt tgctgcagcg gacggaatcg aagaaacagc agcagcagca
     3661 ggagcctgaa aggtaacagc gggatcagcc atgacaacac ctcaactacg caacggcccg
     3721 aagttcggac caagaagtaa cgcgacgagc gcagcaaaga cgctcggcgc gcagctcgac
     3781 gattcgacca gatgagatat cgagatcgga aagacctgcc aaacgcaggt atttgaggtt
     3841 tcgataccag gtagtgcgag gcgtcatctc gcgtgcacgc tcccagccga acgagccaat
     3901 gaggccccac agccccagcg cggaacgcac agcgccgtca gtcagcgact cgccgttgac
     3961 cgttacggaa cgcaaagaag cctcgagatc agatacatgg atcacggacg catcacctag
     4021 aagcttatcg aaatgaaccc aagcagcagc atcgacatca gcagcagaca tttcaaacca
     4081 cggagaaacc ttgttctgtt cattccagaa ctgagaacct aaacgcaact ccagacgtaa
     4141 caaacgattc gcaagagcaa tttcagaatc agaatactga cggccggtgt aatccttctt
     4201 tttcatcaga taggacaaat gcggtccttt cgcatatgcc ttcgccgctt tacgtcgtga
     4261 cgagtggttc caataaacgg tgtcaccagc ctgctgactg acgcgatacc ggccaccctc
     4321 caaattgcgc aaatgagcca gcgccacacg cacagacggc aaatcgtcaa acagcagatg
     4381 aacggtgcaa tccatgcgag aaacccgcca caatgacggg tccaacggca ggattaccga
     4441 caagaccttg caggcatggc gaaccattgc gcgcaggcca tcggctggcc gtaaaccatc
     4501 cacaggcccg aaaacggcat caccgtcgcc aaccacacga cacggcgaac cctgaatgtg
     4561 gatggcatca gagccgacgc gaaacgcaat ttgatgagaa tccgagcgaa cagaatccca
     4621 agccggggat tcccatttca cctcattgga acgcgggcag tagcgcatga tgcggtcgcc
     4681 gatcgtcgac agatgctgcc agacctgttc aggcaacgcc gaaaaaggaa gggacgcagt
     4741 aagccaatca agcatgacca atcttccgta atcggcgggc cgctctggaa aagagcggca
     4801 cggtaaagac gcgacattgc caaacttctg agccgtagga attgccgaac tgtatcactg
     4861 agccgggatt caaaacccgc tgcaggtaat tggcaccctg caaccagcga tcatgcaacc
     4921 cctcaaggtg cggcccctca gtttgaacag ccccaccagt cgggggactc ccacaagcta
     4981 cagacggccc gcgcacggcg acgaccgaac ccggccgaat cccccgcgca cacgccggcc
     5041 acgtctgcgg ccccccccac tgcagcaccg gaacgacgac ggcaccatga gccgcccaga
     5101 agtgccccac tgagcgcgag cgccaaacct gatgcgcggc ccagggctct ggatcggacc
     5161 aagaaatgct gaaatccgga gcaacgacaa taccggcgag cagcgccggc gaaaccgtca
     5221 attcggggcg cctccacgcc gtctcaaagc gccagtcctc aacgaagcaa tgcaatgcca
     5281 cacccgcggg cgcacccgag ccccaccgcg cccagagcaa ggaatcccac tgctgcaacg
     5341 gatcaggccg aatgaaagcg ggcgccggcc agccgtcagc gccgtagacc agcggcagcg
     5401 gacccggcac gcgacgaaca ccgggcctca ttggtcgcgc tcccgctcag cacgccgtgc
     5461 cgctctacgg gcgcgatccc gagcgttgcg cgcgtccttg cgcgcacggt cggggtcaac
     5521 gtcggctgcg gaactctgtg gggctgctgg cgattcctga gcagcccgta ccgcctgagc
     5581 ggcacgggcg acacgacgat cggaggcagc gcggaccgcc ttcgcggcct tcgcaacacg
     5641 ggcagacaca tcgtccggag ccggtacagg tgccggtgcg agtacagaat ccggaacggg
     5701 tgccgaagga ttcagaagcc tggtagcctc agcataatca cgggattcat aagcactgaa
     5761 gattgcatta tgagcccgcg ccttttcgtg atctccccaa agcggtaatt cgccgctgcg
     5821 atgccgatgc tctttgcgaa gttcacgaag gaaagcgccc tgttcatcct gcaatatgcc
     5881 ttcctgttca cggcgcgcca tgacgtgacc gtaattctcc agccgctgat ccgaaagatc
     5941 accgacccaa gaccaatgat cggaatgaac atgctcacgc tgaggaagat cagcaaggaa
     6001 ctgcggttca cgctcagcac gcggttcacg gtcacggccc ctaccgccca tggtgaacca
     6061 cccaatccag ttgctcggga agcgccagaa accacggacc cacatcgcgc gtggccagct
     6121 cgacgacggg ccggccgcga tcgcccggcg tgcccggtgc cgcaccgggc acacgtgcgg
     6181 tccaggcgac gcggcgaacc tgaccgaaac ggccgcgcgc cgctgcaggc gaacgcacac
     6241 gatcgccttc ggtcaaaaac cgccacggtt tcaaaatggc caccgtgaca gggaatagcc
     6301 cgtttcgttc agcgttgcgc gcgcccgctg acaagcagct ttcaaggccg catgcaaatc
     6361 aacatcgctg gaaactgggc aatggtaaaa atcgtcaatg actcgcacgc attcggcgag
     6421 tgaaagagcc agcgtcttgg caatgctcgg gtccggctcg gaaagatcga cctcggtaaa
     6481 accgaagccg tcgcaggatt cgcaaaagtc g
//
LOCUS       DAOQEW010000201         6451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4463_length_6451_cov_8.371013, whole genome shotgun sequence.
ACCESSION   DAOQEW010000201 DAOQEW010000000
VERSION     DAOQEW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_6451_cov_8.371013"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1814
                     /gene="dnaG"
                     /locus_tag="PLS35_16455"
     CDS             <1..1814
                     /gene="dnaG"
                     /locus_tag="PLS35_16455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNI20314.1"
                     /translation="NQIRDRVDIAEIVGQHVALTRAGQNLKGLCPFHQEKSPSFTVSS
                     SRQIFHCFGCGAGGNVYTFLMRLTGAAFPEIVRDLGRKVGVDVPESTGGVSNEARTQL
                     GRLERLNAAAGAWYQRMLTQGPTGAQARAYLDSRGIQQHTTAQFNIGYAPPEWDGLTR
                     AMLKEGFAPADLVAAGLSIPRDQSGSQKTAVSGHYDRFRSRIMFPITDLRKRVVAFGG
                     RILGDGMPKYLNSPETPLFKKGQTLFALDAAREAAGQQHTLIIVEGYFDAIALHQAGI
                     RHVAATLGTALTAEHITVIRRFASNIVLLFDPDQAGVRAALRSLDLFVNSGLSVKVVS
                     LPEGDDPDTYVRQHGAEGFAALHRAAPSLLDFAIEHSLRTAESGSVEDRIRAVDAVLR
                     ILQKSAHPIEREERIRLVAERLGLSQQRLIDRYPTLVSEEQRRPDRTAKAPSISVPSR
                     VKPNPEERDLAHLLVQGSLSAADLRKLSPDAFSAPAYKRLIECALQHLADDGRVSVRT
                     LLDALITDEVCGSLVSELSMQEQHYDDVPAHIAGCLETLERRGRERTMGLLIQELKVA
                     ERERREDEVHRLNGLINDMRIRKAGVMPVALTSAVKE"
     gene            1818..3623
                     /gene="rpoD"
                     /locus_tag="PLS35_16460"
     CDS             1818..3623
                     /gene="rpoD"
                     /locus_tag="PLS35_16460"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNI20315.1"
                     /translation="MPKQELLGEVKKLINLGKEKGFLTYDDLNSTLPADVVSSDQFST
                     IMTMFGEMDIEIVESAETERAPKAAEGEEAIEESEESDSSEENEKEIDLTPGALSRTD
                     DPVRLYLKEMGSVALLSREGEIEIAKRIEEGKKDIATVVYGLPMTLEFVLNLRDQLKN
                     GKIDVREIVPVVETEEDFEEEAVEKDYEELRVKTLDSLNAVRKVSASLKDLYDKARHV
                     GADPEKQKKLRKQIDTVRGQVVDKMESVNLHGVLKDRMTQRVRDLNLLFRQSEREVTS
                     CQRRLGVGGEAGAELLRKLCRTHKDVLAVKRRTGLSEEVLQDIKKHYQAAKGRIRQLE
                     EEEALISAEEIKDAVKHLDVAEEKVKRGKAELVEANLRLVVSIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKL
                     IRTSRHLVQKLGREPTPEEIAERMDLPLDKVRKILKIAREPISLETPIGEEEDSHLGD
                     FIEDKKAVSPLEAAIRYDLQRQINGALETLTPREEKVLRKRFGIGEATDHTLEEVGQD
                     FEVTRERIRQIEAKALRKLRHPSRSKKLRSFVESL"
     gene            3736..3812
                     /locus_tag="PLS35_16465"
     tRNA            3736..3812
                     /locus_tag="PLS35_16465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3770..3772,aa:Ile,seq:cat)
     gene            3890..4666
                     /locus_tag="PLS35_16470"
     CDS             3890..4666
                     /locus_tag="PLS35_16470"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNI20316.1"
                     /translation="MNPQLSPLIELQKLDLRIADLKEQRRRIPERLEVNEAPLREARR
                     TVQDASASIETLNKERRSHEKDLEAHEDRIGKMKDRAAQLKTNQEYQAHLFEVELANK
                     KRGEIEEKILLAMEQIEQTQRMIASAQVQLKDSEALFSKEKAVLDEKDRVLAAELAEL
                     EAQQKELSGRVEKGLLARYNKLKATRKDQALALIKDGICIGCRLQVPPQLISQVKRGD
                     DVYTCPYCYRMLYWEGEPVPEGKRPLVQDQAKDFELGESV"
     gene            complement(4663..5322)
                     /gene="rnhC"
                     /locus_tag="PLS35_16475"
     CDS             complement(4663..5322)
                     /gene="rnhC"
                     /locus_tag="PLS35_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNI20317.1"
                     /translation="MPTTTTLSSLNRIGIDESGKGDYFGPLVIAAVFVTPASEQDLAL
                     MQVRDSKKISDGRILEMAPDIRTVCPHSIVAIGPQRYNELYAKINNLNRLLAWGHARA
                     LENLLQQVDCDLAIADQFGDERLILNALQEKGKQIRLVQRTKAETDLAVAAASILARA
                     EFLLRLQRLSQELHTTLPKGASPAVELAGRMVVKKYGRDRLSTVAKLHFKTTQQVLND
                     T"
     gene            5408..6298
                     /locus_tag="PLS35_16480"
     CDS             5408..6298
                     /locus_tag="PLS35_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNI20318.1"
                     /translation="MKMKSNREGWSKSARPSNLLWLCFGIVTMLVTTGCQTNPYTGRW
                     QLMMMPMSQEVQMGAQAYADVKSDPKMRPSTDPREIEPVKRVAARVIEAAKRSKYSDV
                     ANEFEWEVTVIKDDKTMNAFALPGGKIAVYTGIFPVAKTEAGLAAVMGHEVVHALARH
                     GGERMSQNTLAQTTLQAIGIALGVSGANPVLSQAGMAALGVGAQVGVLLPFSRKHESE
                     ADYVGVLLAADAGYDPREAIQLWQRMGEMSGGKSPSEFMSTHPSHETRIQQLEEWMAE
                     AMPIYQSKPPAPNHELPALH"
BASE COUNT         1469 a         1700 c         1998 g         1284 t
ORIGIN      
        1 tcaaccaaat cagagaccga gtggacatcg cagaaatcgt gggccaacat gtggcgttga
       61 cgcgggccgg gcagaacctc aaaggtctgt gtccctttca ccaagagaaa tctccttcct
      121 tcactgtcag ctcatcgcgg cagattttcc actgtttcgg ctgcggggcc ggcggcaatg
      181 tgtatacgtt tttgatgaga ttaaccggag cggccttccc tgaaatcgtt cgggatttgg
      241 ggcgcaaagt gggggtggat gttcctgaat caaccggcgg agtttccaat gaggcgcgca
      301 cgcaactcgg ccgcttggaa cgcctcaatg cggctgcggg agcctggtat cagcgcatgt
      361 tgacgcaagg gcctaccggc gctcaggcgc gtgcctatct cgacagccga ggcattcagc
      421 agcacaccac cgcgcaattc aacatcggtt acgctccacc ggaatgggac gggctgacta
      481 gagccatgct caaagaggga tttgcgccgg cggatctcgt cgcagccggc ctttctattc
      541 cacgtgatca gagcggctca cagaaaactg ccgtctcagg gcattacgac cggttccgtt
      601 cccggatcat gtttcccatt acggatttgc ggaagcgggt tgtggcattc ggcggtcgta
      661 ttctcggtga cgggatgccc aagtatttga actcccctga aaccccgtta ttcaaaaaag
      721 gccagacgct attcgcgttg gacgcagctc gtgaggccgc aggccagcag catacgttga
      781 tcatcgtcga aggctatttt gatgccattg cgttgcatca ggcgggcatt cgccatgtgg
      841 cggcgacctt gggaacagcc ttaaccgctg agcacattac ggtcattcgc cggttcgcgt
      901 cgaatatcgt gttgctcttc gatcccgatc aggcaggggt tcgggcggca ttgcgctcgc
      961 tggatttgtt cgtcaatagc ggcttgagcg tcaaggtcgt ctcgcttcct gagggtgacg
     1021 atccggatac gtatgtccgg caacatggcg ccgaagggtt tgcggccttg catcgcgcgg
     1081 cgccaagcct gttggatttt gccattgagc acagcctccg cacggcggag tcgggcagcg
     1141 tggaagaccg gattcgggct gtggatgcgg tgttgcggat tcttcagaaa agtgcgcatc
     1201 cgatcgagcg cgaagagcgg atcaggctcg ttgcagaacg attgggactc agtcagcagc
     1261 gcctcatcga tcgctatccc acgctcgtct cggaagagca gcgtcgcccg gaccggacag
     1321 ctaaggctcc ctccatctca gtcccgtcca gagtgaagcc caatcctgaa gagcgcgatc
     1381 tcgcgcattt gcttgtgcag gggagtttgt ctgccgcgga tctccggaaa ctgtcccccg
     1441 atgcgttttc ggcaccggcg tacaagcggt tgatcgaatg tgccttgcag catctggcag
     1501 acgatggcag ggtgtccgtc aggaccctgc tggatgcatt gatcacggat gaggtctgcg
     1561 gctcgttggt gtcggaactg tcgatgcagg agcagcatta tgacgacgtg ccggcccata
     1621 ttgccgggtg tttggagacc ctggagcggc gaggccgcga acggactatg ggcctcttga
     1681 ttcaagaatt gaaggtcgcg gaacgcgaac gacgggaaga tgaagtgcat cgcctgaacg
     1741 gattgattaa cgatatgcgg attcggaaag ccggtgtgat gccggttgcg ctgacgtctg
     1801 cggtgaagga gtagggtatg ccgaaacaag aattactcgg cgaggtgaag aagcttatca
     1861 atctcggcaa ggagaagggc ttcctcacgt acgacgatct caacagcacc ttgccggcgg
     1921 atgtcgtctc gtcggaccaa ttcagcacga tcatgaccat gttcggcgaa atggacatcg
     1981 agattgtgga gtccgctgag acggagcgcg ctccaaaggc cgccgaaggc gaggaagcga
     2041 ttgaggagag cgaagaatcc gactcctcgg aagaaaacga aaaggagatc gatctcaccc
     2101 cgggggcgct cagccgcacc gacgaccctg tccggctcta cctgaaagaa atgggcagtg
     2161 tggctctgct gagccgcgag ggcgaaatcg agattgccaa gcgaatcgaa gaaggcaaga
     2221 aagatattgc cacggtcgta tacggactgc cgatgaccct ggagttcgtc ctgaatctcc
     2281 gcgatcagct gaaaaacggc aagattgatg tgcgcgagat cgtgccggtt gtggagacag
     2341 aagaggactt cgaagaagaa gccgtcgaaa aggattacga agagctccgg gtgaaaacgc
     2401 tggattcttt gaacgccgtg cgcaaggtat ctgcctcgct caaggatctc tacgacaagg
     2461 ctcgtcatgt cggtgccgat cccgaaaaac aaaagaagct gcgcaagcag atcgacacgg
     2521 tgcgtggcca ggttgtggat aagatggagt cggtcaatct gcatggcgtt ctgaaagacc
     2581 gcatgaccca gcgggtacgc gatcttaacc tgctgttccg tcaatcggag cgggaagtca
     2641 cgagctgcca gcgtcgtctc ggtgtcggcg gcgaggccgg cgccgaattg ctgcggaagt
     2701 tgtgtcgaac ccataaagat gtgctcgcgg tgaagcgcag gaccgggctc tccgaagaag
     2761 tccttcagga catcaaaaaa cactatcaag ccgccaaggg ccggatccgc caacttgaag
     2821 aggaagaagc gctgatttcc gctgaagaga tcaaggatgc cgtcaagcat ctcgatgtgg
     2881 cggaggaaaa ggttaaacgc gggaaggcgg aattggtcga ggccaatctg cgtctggtgg
     2941 tgagtatcgc gaaaaagtat acgaaccgtg ggctgcagtt cctggatctg attcaggaag
     3001 gcaatatcgg cctcatgaag gcggtcgata agtttgaata caaacgtggg tataagttca
     3061 gtacctacgc aacctggtgg attcggcaag ccatcacccg tgcgatcgcc gaccaggcgc
     3121 gaaccatccg tattccggtt cacatgatcg aaacgatcaa taaactgatc aggacctcgc
     3181 gccatctggt gcagaaactc ggtcgcgaac cgacgccgga agaaattgcc gaacgcatgg
     3241 atttgccgct cgacaaggtg cgcaagattc tgaaaatcgc ccgtgagcca atttctctcg
     3301 aaaccccgat cggcgaagag gaagacagcc acttaggaga cttcatcgaa gacaagaagg
     3361 cggtctcgcc attggaagcc gccattcggt acgacttgca acgtcagatc aatggcgcac
     3421 tggaaaccct cactcctcgg gaagaaaagg tgttgcggaa acgctttggc atcggcgaag
     3481 cgaccgatca cacgcttgaa gaagtcggac aggattttga ggtgactcgc gagcgcattc
     3541 ggcaaatcga ggcgaaagcg ttgcggaagc tccggcatcc gagccgtagc aagaagctgc
     3601 gtagtttcgt ggagagtctc tagtcttcgt cgtcaggagt ggtttgactc tttccgactg
     3661 gcagctttag aatcaggccg ctcgaaccag gtatgccgtg atgcaggctg cggccaggtc
     3721 actggcggca gggctgggcc catagctcag gtggttagag cggctgactc ataatcagct
     3781 ggtcctaggt tcaagtccta gtgggcccac cagcctgagc gcctcaccat cattccggtg
     3841 cgagaggcgt cgtgccgacg ttgctctcgc gcaactcaca aggagcccgt tgaacccaca
     3901 gctttcccct ctcatcgaat tgcagaaact cgatctccgc atcgccgacc tcaaagaaca
     3961 acggcgaaga atacccgaac ggcttgaagt gaatgaggct cctctgcggg aagcaaggcg
     4021 gacggttcaa gacgcgtcgg cttcgatcga gaccttgaac aaggaacggc gaagtcacga
     4081 gaaggacctg gaggcgcacg aggaccgcat cgggaagatg aaagatcgcg ccgcccagtt
     4141 gaagacgaac caggaatatc aggcccatct gttcgaagtc gagctggcca ataagaaacg
     4201 cggagagatc gaagagaaga tcctgctcgc gatggagcag attgaacaga ctcaacggat
     4261 gattgcttcc gcccaggtgc aactgaaaga ctcagaagcc cttttcagca aagagaaagc
     4321 ggtgctcgac gagaaggacc gcgtgctcgc tgcggaactc gccgagttgg aagctcaaca
     4381 aaaggaattg tcaggacggg ttgagaaggg cctgctcgct cggtataaca agctgaaagc
     4441 cacgcgaaaa gaccaggcgc tggcgctgat caaggatggt atttgtatcg ggtgccggct
     4501 ccaggtgccg cctcaattga tttctcaggt caaacgcggg gacgacgtct atacttgccc
     4561 ctattgttat cgaatgttgt attgggaagg tgagcctgtt ccagagggaa agcgccctct
     4621 cgtccaagac caggcgaagg attttgagct cggcgaatcc gtctaggtgt cgttcagcac
     4681 ctgctgtgtt gttttaaagt gcaatttcgc gacggtactc agtctgtccc gcccgtactt
     4741 tttcacgacc atacgtccgg ccaattccac ggcaggggat gcacccttgg gtagggtcgt
     4801 gtgtaattcc tgcgagagcc gttggaggcg caataaaaat tcggcgcgcg caagtattga
     4861 tgcggccgct acggccaagt ccgtttctgc tttcgtgcgt tgcaccaacc ggatctgctt
     4921 gcctttctcc tgaagcgcgt tgagtatcaa tcgttcgtcg ccgaactggt cggcaatcgc
     4981 cagatcgcag tcgacttgct gaagcaggtt ttccagcgcc cgtgcatggc cccaggccaa
     5041 gaggcgattg aggttgttga tcttggcata gagctcattg tagcgctgcg ggccgatcgc
     5101 cacgatgctg tgggggcaca ctgtccggat gtcgggggcc atttcgagaa tacgcccatc
     5161 ggaaatcttc ttgctgtcac gaacctgcat gagggcgaga tcctgttctg atgcaggcgt
     5221 gacgaacacg gcggcaatca ccagaggtcc aaaataatcg cctttgccgg attcgtcgat
     5281 gccaattcga ttcagcgagg agagtgtcgt ggtggtaggc actcggtgaa actcccagta
     5341 gaaacgggtg gggcggcgcg gtaatctagc agagcatatc agactggtca atttgcggag
     5401 gcctgtgatg aagatgaaat cgaaccgtga ggggtggtcg aaatctgccc ggccatcgaa
     5461 cctgctgtgg ctgtgtttcg gcatcgtgac catgttggtg acgactggtt gccaaaccaa
     5521 tccctacacc ggacgctggc agctgatgat gatgccgatg tcccaggaag tgcagatggg
     5581 cgcgcaggcg tatgccgacg tcaagagcga tcccaaaatg aggccctcca ccgaccctcg
     5641 ggagatcgag ccggtcaaac gtgtcgccgc gagagtgatc gaggccgcta aacgatccaa
     5701 atacagcgac gtggccaatg aatttgaatg ggaagtgacg gtcatcaagg acgataagac
     5761 catgaacgcc tttgccctgc cgggcgggaa aatcgcggtg tataccggaa ttttccccgt
     5821 agccaagacc gaggccggac ttgcggcggt gatggggcat gaagtcgtcc atgcgttggc
     5881 tcgtcacggc ggagaacgga tgagtcagaa cacattggca caaaccacct tacaagcgat
     5941 cggcatcgcg ctgggggtga gtggcgccaa tccggtgctc tcgcaggcag gcatggcggc
     6001 attgggtgtc ggtgcgcagg tcggggtgtt gctgcccttc agtcgcaagc acgaatcaga
     6061 ggcagactat gtcggtgtgt tgctggcggc ggatgccggc tatgacccgc gtgaggcgat
     6121 ccagttgtgg caaagaatgg gcgagatgtc cggaggtaaa tctccgtccg aatttatgtc
     6181 cacccacccg agccacgaaa cccgaattca acagctggag gagtggatgg cggaggccat
     6241 gcccatctat cagtcgaagc cgcctgcacc caaccacgag ttgcccgcat tgcattaagt
     6301 ccgcccggct tcttacttga atcggcctgg ggggactcgt atactttcct ccgcgcgctg
     6361 gggggacacg ggtgctcgct cgccgagcct gctcggcggg aggaaagtcc ggactccaag
     6421 ggcaaggacg ctggataacg tccaggcgga g
//
LOCUS       DAOQEW010000202         6431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4481_length_6431_cov_5.570107, whole genome shotgun sequence.
ACCESSION   DAOQEW010000202 DAOQEW010000000
VERSION     DAOQEW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4481_length_6431_cov_5.570107"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLS35_16485"
     CDS             complement(<1..761)
                     /locus_tag="PLS35_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20319.1"
                     /translation="MAVTHDEPASMLKQEDFVFTNSSLSQEPANTPVWRSDRSVRVFV
                     LSIWLGMVMLAMVNWAEGSRNVPFSEDWLLVSALMGHEPYLGTWLWSQHNEHRVPVPR
                     LILLALLTVGQGDFRIGGAFNILALGGVSLLMMETARSVRGGSSRVADAFFPVAFLHL
                     GHTENMLWTWQITQVLPVILVSALMLIVVAGRIPRTTASTLAAGVCTIMLPLCGANGL
                     LYAPLFAFWIGYVGIVIIAGRSNEHIAGENWKGRY"
     gene            complement(767..2299)
                     /locus_tag="PLS35_16490"
     CDS             complement(767..2299)
                     /locus_tag="PLS35_16490"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HNI20320.1"
                     /translation="MDDLRDTRIDTLALREKYRDQYFLHRDPIYQDRLLWRAHTFRHI
                     MHLLPTQSILELGCGRGLFTPHLCRVSRGENPVTSVTFRAQQARPDSLSADVEFLYIS
                     ALPGPLRGKHYDFIVAIDLLDQRNCGWFLQQIYELLRPGGQVLFYESNPWNLALRAKH
                     CWRRLRGGDDPRRLLNRPVLYELMSEVGFIRVFALYNDFVYAPLTRSLIWFLRNMSIL
                     LENARGIQTFAGSILLHAQKPPRTTVRPLRSLCEHAQLHRAISVVIPCHNEEMNVEPL
                     VTQLMALYGDYIHEIIPVDDNSTDRTAEVLRRLAGAEPRVKPVFRTPPNGVGRALADG
                     YRVATGQYVLSMDCDFQHLLPEIRDLFDAAAQGYDVVIGSRFSRHSVLLNYPFKKILA
                     NRAFHALGQILFLRQFRDLTNNLKLMRREVVERLQLTQPHFAVNAETGLQPLLMGYSL
                     REVPISWINRTPDMGSSSFHLGKVGRGYMAVLFRLWWKHLAGAIAVPLKMKSSNSESS
                     LR"
     gene            complement(2311..4314)
                     /locus_tag="PLS35_16495"
     CDS             complement(2311..4314)
                     /locus_tag="PLS35_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20321.1"
                     /translation="MTAHNIPAVDSLPHREEPSTDQISADFRMLLGAVDVVSFDLFDT
                     LIQRDALFCPKDLFYRVQAVVEHRHAITLKDFVSTRVRAESIARVRARGRQSEETTLE
                     DIYVEMANILSLPSSFAEEMLHIELECERAALMALPDGKRLYDAAIRAGKTTVVISDT
                     YLSRVFLEEILMKAGFNGVHRVYASSEFGKTKHHGALFGIVLKDLKCSPGRLLHVGDN
                     PLSDATAALGQGLRSFICTNPKQRLKWRYGLSDRPSGSQAISALLCDISRTAEEQSHG
                     TTDRAVIARSAVEHLSWVYEGFAAWLVHQMASQRDTRVYFASRDGLIMKKFFDVTRKA
                     FGTPIESRYLYVSRAALYPMLVLTDPDMARRLFCHSWDHLTMTQALKRMSLVPQEVAG
                     QLAKYGLADPLLSLNRVTTLKLSRFLDAIWPLLCAKSESHCELVAEYLRQEGFLADEP
                     TAFVDIGWHASLQHCLVKLLQHRGFKNTLHGYYLGTFARPDASPEVSAKGFLIENDAP
                     ASISALIRTGPSLLELFHAAGHGSVLGYERKDGRVVPVLEENEREQEQFRRIIEPLQQ
                     HAFEFVAARLARGDKPEASTFPPELVARLGLRILHKPTDEEAEIFGRLRIATDFGGSM
                     KSLTGACEWNLSEVVGDALPDGIPPMWYPGFYALKKSATKRAS"
     gene            complement(4311..>6431)
                     /locus_tag="PLS35_16500"
     CDS             complement(4311..>6431)
                     /locus_tag="PLS35_16500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20322.1"
                     /translation="RALAVARGDIVAFTDDDVIVDQRWVRTLAEVFVRHPEVMAVTGL
                     VVPAELETRAQILFETYGGFGRGFEQRWYRAHSTKPQVDTFHLGAGVFGTGANMAYRR
                     ALFKNLNGFDPALDVGTVTNGGGDLEMFFRVLQEGYVLVYEPRALVRHRHRRTYQELA
                     TQLTNHGVGLYSCLSRCVRAYPALRPAGVCFGARWFWSWNIRRLLVSYVRRSSLPRDL
                     MVAELRGSLRGLFAYQTARRTARRLRDLQAGTGERAEEDMFLKLSSDLSHSPTGQPGK
                     ASCSLLPKPQFPIAVRAVELSEPLSDIGDLEKYKALQLYVTLHGELLGKHTIEHTAGI
                     VPVAQLRQAIAKSFGIRLLSRDTHHLGDAPWNRIIADLVERSPIPNPQPPKLSAGSRV
                     SIVIATLDRPEELRHCLGALSRQESSRQVEIIVVDNNPASGLTQTILKEFPAVVSVQQ
                     ARRGLSYARNSGIVKSSGEIIIMIDDDVTVSSTWLERLIGHFSRPDVMAVTGNVLPLE
                     LDTEPQVLFETYGGLGRGFERLDVNGSWLRAFYSAPRTWVLGATANAAFRASVFRDEH
                     IGLLDEALGAGMAGVGEDTYLFYRILRANHRLVYEPSAYVWHKHRRDIRAFRRQLFAY
                     SKGHVAYHLTTLFRDGDLRVLLYLFVSLPRAYLFRAKSRLLGRTVYPLSLLLLEILGN
                     LIGPWTYVACRWKASRHSAERVSR"
BASE COUNT         1468 a         1883 c         1821 g         1259 t
ORIGIN      
        1 aagtaccgac ctttccagtt ttccccggcg atgtgttcat tcgagcgacc ggctatgatc
       61 acgatcccca cgtaaccgat ccagaaggca aacagcggcg catacagcaa cccgtttgcc
      121 ccgcagagcg gcaacatgat ggtgcagaca ccggcggcaa gtgttgatgc ggtggtccga
      181 ggaatccgcc cggcgaccac gatgagcatg agggcggaca ccaagatgac gggaagaacc
      241 tgcgtgattt gccaggtcca cagcatattc tctgtatgcc caagatgaag gaacgccacg
      301 gggaagaacg cgtccgcgac tcgcgacgag ccaccgcgaa cggatctcgc cgtttccatc
      361 atcaacagcg acactccgcc gagggcgaga atgttaaagg ctcctcctat ccgaaaatca
      421 ccctgtccca ctgtcaggag cgcgaggaga atgagccgcg gcacgggaac acgatgttcg
      481 ttatgttgtg accacagcca tgtgccgaga tacggttcat ggcccatgag cgccgagacc
      541 agtaaccaat cctcactaaa cggcacgttt cgactgcctt cggcccaatt gaccatcgcg
      601 agcatcacca tccccagcca gatgctcaac acgaacaccc gcacggacct gtccgatcgc
      661 cagactggag tattggcagg ctcttggctg agtgacgaat tcgtgaagac gaaatcctcc
      721 tgcttcagca tgcttgccgg ttcgtcatgc gtgaccgcca tggccgctat ctcaagctcg
      781 attcagagtt tgacgatttc atcttgagag ggaccgcgat cgctcctgca agatgtttcc
      841 accacaaccg gaacaagacc gccatgtagc cccggcctac cttgccaaga tgaaaggaag
      901 agctccccat atcgggtgtc cggttgatcc atgagatcgg cacctcccgg agtgaatagc
      961 ccatcagcaa cggttgcaga ccggtttcgg cattcacggc aaaatgcggc tgtgttaatt
     1021 gaaggcgctc gaccacctcc cgccgcatca atttcaaatt gttggtcaga tcccggaact
     1081 gacgtaaaaa caggatttgc ccaagggcat gaaacgctcg attagccagg atttttttga
     1141 acgggtagtt gagcaacacg ctatggcgcg aaaaacgact gccgatcacc acgtcgtacc
     1201 cctgtgctgc cgcatcgaac agatccctga tctcaggcag gagatgttga aaatcgcagt
     1261 ccatagacag gacgtactgc cctgtcgcta ctcggtagcc gtcggccagc gctcgtccca
     1321 ccccgttcgg cggagtccga aaaaccggtt tgacgcgtgg ctcggcgccg gccaggcggc
     1381 gcagtacctc ggcagtacga tccgtgctgt tatcatcaac cggaataatt tcatggatat
     1441 agtccccgta gagcgccatc agttgcgtaa ccagcggctc aacattcatc tcctcgttat
     1501 ggcaagggat gacgaccgag atcgcacgat ggagttgcgc atgttcgcac agggaacgca
     1561 gcggtctgac ggtagtgcgt ggcggcttct gggcgtgcag gaggatagag ccggcaaagg
     1621 tttggatgcc cctggcgttc tcaaggagaa tcgacatgtt gcgtagaaac cagatcagcg
     1681 aacgggttaa cggggcatat acgaaatcgt tgtacagggc gaatacgcga atgaatccga
     1741 cttcggacat caattcgtag agaaccgggc gattcaacag gcgacgcgga tcgtcgccgc
     1801 cgcgcaggcg gcgccagcag tgttttgctc gcaacgccag gttccaaggg ttgctttcat
     1861 aaaaaaggac ttggccaccc ggccgcaaca actcatagat ctgctgcaag aaccatccac
     1921 agttacgttg atccagtaaa tctatggcca cgatgaaatc atagtgtttc ccccgtagcg
     1981 gaccgggaag cgcggagatg taaagaaact caacgtcggc agagagagaa tcggggcgag
     2041 cctgttgagc ccggaacgtg acggacgtga cgggattctc tcccctcgac acacgacaga
     2101 gatggggcgt gaacaatccg cgcccacagc ccagttccaa aattgattga gtgggcagga
     2161 gatgcatgat gtgccggaac gtatgcgctc gccataacaa acggtcttga taaatgggat
     2221 cgcgatgcag aaagtattga tcgcggtatt tttccctgag cgccaatgtg tcaatgcgag
     2281 tatcgcgaag gtcgtccatc acaatggtcc ctacgaggct cgcttagtgg ccgatttttt
     2341 tagcgcataa aatcccggat accacatggg agggatgccg tcaggaagcg catctcccac
     2401 cacctccgag aggttccatt cgcaagcgcc cgtgagactc ttcatgctgc cgccaaaatc
     2461 ggtggcgatt ctcagtcgtc cgaaaatttc agcttcctca tcggttggct tgtggaggat
     2521 gcgtaacccc aagcgggcga ccagttcagg cggaaacgtc gaggcctccg gcttatctcc
     2581 tcgggcgagg cgcgccgcga caaactcaaa ggcgtgctgt tgtaacggtt cgatgatcct
     2641 gcggaattgt tcctgctcgc gctcgttctc ttccaggacg ggtacgacgc gaccgtcttt
     2701 acgctcgtat cccagcacgg atccatggcc ggcggcatgg aatagttcca acagggaagg
     2761 accggtccgg atcagggcgg agatcgatgc gggcgcatca ttctcgatca agaagccttt
     2821 cgcagagacc tccggggatg cgtccggcct ggcaaaggtc cccagataat agccgtgaag
     2881 agtattcttg aaaccgcgat gctgcagcag cttcacaagg cagtgctgta gcgacgcgtg
     2941 ccatccgata tcgacaaatg ccgtaggctc gtcggccaag aatccctcct gtcgaaggta
     3001 ctccgccacg agttcgcaat gactctcact ttttgcgcag agtaacggcc agatggcatc
     3061 gaggaaccgg ctcagcttca atgtggtgac tcgattcaac gagagcagag gatccgccag
     3121 gccatatttg gccagttgcc cggcaacttc ctgaggaacc aaggacatcc gctttaaggc
     3181 ctgtgtcatc gtcaggtggt cccagctatg gcagaacaac cgtcgcgcca tgtcgggatc
     3241 ggtgagaacc agcatcggat agagcgctgc ccgtgagaca tagaggtagc gactctcaat
     3301 cggggtcccg aaggcctttc gcgtgacgtc gaagaacttt ttcataataa ggccgtcacg
     3361 agaggcgaag tagactcgtg tatccctctg actcgccatc tgatgcacta accaggcggc
     3421 gaacccctca tacacccagg agagatgctc cacggcactc cgagcaatca cggcacggtc
     3481 ggtcgtgccg tgagattgct cctctgcagt tcggctgatg tcgcacaaca gcgccgaaat
     3541 ggcctgactc ccgctgggtc tgtcgctgag cccatagcgc cacttcagcc gttgtttcgg
     3601 gttggtgcag atgaagcttc ggagtccttg acccagcgcg gcagtggcgt cggacagggg
     3661 gttatcgccg acgtgaagca gccggcccgg cgaacacttc aaatctttca gcacgatgcc
     3721 gaagagcgcg ccgtggtgct tggtctttcc gaattccgag gaggcataca cgcgatgcac
     3781 cccgttgaat ccagccttca tgaggatctc ttcgagaaac acccggctca aataggtgtc
     3841 cgatatcacc acggtggttt tgcctgcgcg gattgcggcg tcgtagagcc gctttccatc
     3901 cggcaaggcc atcagggctg cccgttcgca ctcaagttct atgtgcagca tctcctcagc
     3961 gaaactgctg ggcagactca ggatattggc catttcgacg tagatgtctt cgagcgtcgt
     4021 ttcctcactc tgccgtcctc gcgctcgaac acgggcgata ctctccgccc gtactcgggt
     4081 agaaacgaaa tctttgagtg tgatggcatg gcgatgttcg acgacggcct gaacccgata
     4141 gaagagatct ttgggacaga acagggcatc ccgttgaatg agcgtgtcga acagatcaaa
     4201 cgacaccaca tcaaccgccc cgaggagcat gcggaaatcg gcagatatct ggtccgtgga
     4261 aggctcctcg cggtgaggca ggctatcgac cgccgggatg ttgtgagcgg tcatcgtgag
     4321 accctctcgg ctgagtgccg gctggctttc cacctgcacg cgacgtatgt ccagggaccg
     4381 atcagattgc cgaggatttc gagcagcagc aaagacaacg gatacacagt tcgccccagg
     4441 agtctggact tcgccctgaa cagataggcc cggggaagcg acacgaagag atagagcagg
     4501 acgcgcaaat ccccatcccg gaacagcgtc gtgagatggt aggccacatg ccctttgctg
     4561 taggcaaaca gttgacgacg aaaggcacgg atgtctcgtc ggtgcttgtg ccagacatag
     4621 gccgatggtt catacaccaa tctgtggttg gctcgaagta tgcggtaaaa caggtacgtg
     4681 tcttctccga ctccagccat cccggcgccg agcgcttcgt ccagcaaccc gatgtgctcg
     4741 tcacgaaaga cgctggctcg aaatgcggca ttggccgttg cacccaacac ccaggttctc
     4801 ggagccgagt aaaacgcacg cagccagctt ccgttcacat ccaaccgttc aaaccctcgc
     4861 cccaatcctc cataggtctc aaacaacacc tgcggctccg tgtcgagttc cagaggaagc
     4921 acattgccgg tcaccgccat gacgtctggc cgagaaaaat ggccgatcaa ccgttccagc
     4981 cacgtcgaag acaccgtgac atcgtcatca atcatgatga tgatctcgcc ggagcttttc
     5041 acaatgcccg aatttctcgc gtaactgagt cccctgcgag cctgctgcac cgaaaccacc
     5101 gcgggaaact ccttcagaat cgtctgcgtg agaccggacg ccgggttatt gtcgaccaca
     5161 ataatttcga cttgccgtga agactcctgg cgtgacagtg cgccgagaca gtgtcgaagc
     5221 tcttccggac ggtcgagcgt ggcgatcaca atggaaaccc ggctgccggc tgacagtttg
     5281 ggaggctgcg gattcggaat cggactccgc tcaaccaggt ctgcgatgat tctgttccag
     5341 ggtgcgtcgc cgagatggtg tgtgtctcgt gagagcaggc gtatgccaaa cgatttggcg
     5401 atggcttggc ggagttgagc gaccggcacg atccccgcag tatgctcaat ggtgtgtttc
     5461 ccgaggagct caccgtgcag ggtcacgtac agctgcagcg ctttgtattt ctccagatca
     5521 ccgatatcgc tcaatggctc actgagttcc acggcacgta cggcgatggg aaattgcggc
     5581 ttcggcagca gactgcaaga cgctttgccc ggctgcccag tcggcgaatg cgagaggtct
     5641 gacgagagct tgaggaacat gtcctcttcg gcgcgttcac ctgtacctgc ctgcagatcg
     5701 cgcagcctac gcgctgtgcg tcgagccgtc tgatatgcaa agagaccgcg gagcgatccg
     5761 cgcagttcgg ccaccatcaa gtcgcgaggc aatgacgaac ggcgcacgta gctcacgagg
     5821 agtcggcgaa tattccatga ccagaaccat ctcgcgccaa aacacacccc cgcgggacgg
     5881 agggcgggat aggcacgaac gcatcgggac aaacaggagt acaaccccac gccgtgattg
     5941 gtgagctgcg tcgctaattc ttgatacgtt cgcctatgcc ggtggcggac gagggctcga
     6001 ggttcataga cgaggacgta gccctcctgt agcacgcgaa aaaacatctc gagatcaccg
     6061 ccaccattgg tgaccgtccc cacatcgaga gctggatcga acccgttcag atttttgaac
     6121 agggcacgac gatacgccat gttggcgccg gtcccgaaga caccagcgcc aagatgaaac
     6181 gtatcgacct gcggctttgt agaatgggct cgataccacc gttgctcgaa cccgcggccg
     6241 aatcccccat acgtctcaaa cagtatttgc gcccgggttt ccaattcagc gggcaccact
     6301 aacccggtta ccgccatcac ctccggatga cggacaaaca cttccgcgag ggtgcgcacc
     6361 catcgctggt cgacaatgac atcgtcatcc gtgaacgcca cgatgtcgcc acgtgccacc
     6421 gccagcgccc g
//
LOCUS       DAOQEW010000203         6394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4527_length_6394_cov_10.220855, whole genome shotgun sequence.
ACCESSION   DAOQEW010000203 DAOQEW010000000
VERSION     DAOQEW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_6394_cov_10.220855"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLS35_16505"
     CDS             <1..811
                     /locus_tag="PLS35_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNI20323.1"
                     /translation="VLKDELIPYTIVPAYDQDPTYYYDWEGEAEFVLEDLGPGECAGG
                     ALEMIDDRMLEADQELYQAKLLVEKHQYALSVNKSYRAVLAAAKGLLVTEGIDPATDA
                     ETFQEFDVRLASKGIVPASYTNLGAQVGDLGSKDATAETANDKMAFAKRFLSVCRAAT
                     EQMGKDLKLAQVKEEVVPTPAPKVEPASPVATAPVYDLRGVACPMNYVKTKLKLEMMD
                     NGEQLEVWLDAGEPIRNVPMSLRNDGHKILEEGPLEPEAKHFKILVEKVEG"
     gene            818..1540
                     /locus_tag="PLS35_16510"
     CDS             818..1540
                     /locus_tag="PLS35_16510"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNI20324.1"
                     /translation="MQDTQKSAERPSDAELKALSDSFESQQPWDLLAYALKTYQGRIV
                     LACSFGAEDVALVDMVHRIDPDAPLFYLDTDFLFPETFDVRDRIVARYGLKPAQVIQM
                     KSLLTPEQQAAQHGAALWASKPDQCCELRKIEPLTRILGQYSAWITGIRRDQAPTRAN
                     AGLIEWDKKFQLVKVNPLAAWSTEQVWTYIQLHEVPYNILHDRNYPSIGCTHCTAPVL
                     PGDDPRSGRWKNFGKTECGLHK"
     gene            1583..2650
                     /locus_tag="PLS35_16515"
     CDS             1583..2650
                     /locus_tag="PLS35_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNI20325.1"
                     /translation="MQHLLAKVAKGQKTSKDLTWEEAKQAMRLMIEGTATQAQIGAFL
                     TAMRFKSESVTELAAFTATARQYVPPVPVRSGLGVVDVPVYAGKRETFHAIVPAAIIA
                     AAAGAVVLLHGVDGPADRRGVSSVLKLLGIPTDQTAKLVGPELEKKGFAYLDLALYHP
                     PVSRFLEMRQELGVRNFFHPVARMLNPARAASQVIGLSHPPYFEKTIEALRMLSCPRA
                     LVIRGVEGDPELSIGNSTRLLELKDERITPFTFQPKDAGLAMATFREMAGFPAEQRER
                     EADLIKRLVANEIQGGQRDWVLLNAAMLLYAAGKGTSITGNLAAARNALESGRAKAKL
                     SELIAGPGSETAAGSKVGLSA"
     gene            2668..3465
                     /gene="cysD"
                     /locus_tag="PLS35_16520"
     CDS             2668..3465
                     /gene="cysD"
                     /locus_tag="PLS35_16520"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNI20326.1"
                     /translation="MKHLRQLEDQSVYILREAYKHFNHLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPAMIEYRDRIAREWRLNLVVGQNKEALAAGMNHTLGRDV
                     CCTALKTNAMKNLVEEKGYTGIILGVRADEDSTRAKERYFSPRDKHGDWDFRDQPPEL
                     WDQFKTTFPPGTHIRIHPLLDWTEINIWEYIKHENIPFMDLYLDRGDGTRYRSLGCAP
                     CTMPIKSTAKTVDEIIVELRKSTVAERAGRAQDAGRGMEMLRKKGYM"
     gene            3540..5387
                     /locus_tag="PLS35_16525"
     CDS             3540..5387
                     /locus_tag="PLS35_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNI20327.1"
                     /translation="MTQHDQTKPQESLNIVIVGHVDHGKSTLVGRLYADTGSLPEGKL
                     EKVQAICRQQGKEFEYAFLFDAFLEEQEQGITIDTARTFFIWNGRQYIIIDAPGHKEF
                     LKNMISGAARAEAALLLIDALEGVREQSKKHGYLLSLLGVTQFAVVVNKMDLVGYRQD
                     VFDGIEKEYREFLGQFRAVPQQMIPVSAKMGDNIATRSTHMNWYQGPTVLETLALFKK
                     EQVRSEQPLRFPLQDVYKFDARRILAGRITAGRLKVGDQLVFSPSNKTAVVQSIEGFN
                     VDPPPSEAQAGQSVGITLDEQIFVERGEIASHRDSVPLVSTAVRVNLFWMGRRPLEKG
                     RKYVLRLATREVACEVAVIHRIIDTADLAQLQESQSVARNQVAELTLRVKSPLAFDLS
                     SSFESTGRFVLVDEYDISGGGIITELVHDEQEGLREEARQREYAWLTGDVRPEDRALQ
                     YGHRAAIVLFTGSAQTGKTFLARRVEALLVADSRHAYLLEGENLLQGLDADLSAADPS
                     FASERVRRYGEVARLLIDTGLIVVSTSKTFGINYQRMAEMIRTLVQPAPVIAVHMSRA
                     GEEPPPNTDLHFAGPQDFDAAARQIIEELKKRGVLIQPSGTKSNIQYSI"
     gene            5466..6392
                     /locus_tag="PLS35_16530"
     CDS             5466..6392
                     /locus_tag="PLS35_16530"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNI20328.1"
                     /translation="MTMAAGKDLKSILTKLQYSDDAKVVQQITAQMKQVQARMAGIKQ
                     KLVVMSGKGGVGKSMTTVNLALAFARQGAKVGLLDVDLNGPCVPRMMGLHGQSLTMTP
                     EGAQPPVGPLGIKVASMDFFLGDASPVRWKGPMDLSPVWLGLMEMNVIREFLADVVWG
                     ELDYLLADLPPGAAADKPPVIAGFIPDLAGAIVVTTPSEVASDVVQKSVTYARDMGIR
                     VLGIVENMSEYRCPSCGAENELFEGNTEAMCEVLDLPLLGRIPFDRTLAKTFDKGQPL
                     LDPDYPTIRKYQEIVGRIQSLLDYKKVLAEKL"
BASE COUNT         1417 a         1670 c         1960 g         1347 t
ORIGIN      
        1 cgtcctcaag gacgaactca tcccctatac gattgttcct gcgtacgacc aggatccgac
       61 ctactactat gactgggaag gtgaggccga atttgtgttg gaggatctgg ggcccggcga
      121 atgcgcaggt ggagccttgg aaatgatcga cgaccgcatg ttggaagccg atcaggagct
      181 ctatcaagcg aaactacttg tggagaagca tcaatacgct ctgtccgtga ataagtccta
      241 tcgcgcggtc ctggcggcgg ccaaagggtt gttggtcacc gaaggaatcg atccggcgac
      301 cgatgcggag acattccagg agttcgacgt gcgcttggcg agcaaaggca ttgtacccgc
      361 ctcctacacc aacctcggtg ctcaggtggg ggatttggga tccaaggatg ccacggccga
      421 aacggcgaat gacaagatgg cgtttgccaa gcggttcctc tcagtctgcc gggcggccac
      481 cgaacaaatg ggcaaggatt tgaagttggc gcaggtgaag gaagaggtgg ttccgactcc
      541 ggctcccaaa gtggagcctg cgagccccgt tgccaccgcg ccggtctacg atttgcgcgg
      601 tgtcgcctgc ccgatgaatt atgtgaagac caaattgaaa ttggaaatga tggacaatgg
      661 cgagcagctc gaagtctggc tcgatgccgg agagccgatt cgcaatgtgc cgatgagtct
      721 acggaacgat gggcacaaga ttcttgaaga aggcccgttg gagccggagg ccaaacattt
      781 caagattttg gtggaaaagg tcgaggggtg agaaagagtg caagacacac agaagtccgc
      841 tgaacgacct tccgacgcag agctgaaggc gctgagtgat tcgtttgaat ctcagcagcc
      901 gtgggacctg ctggcgtatg ccctgaagac gtatcagggc aggatcgtgc tcgcctgtag
      961 cttcggggcg gaagatgtag cgttggtcga tatggtgcat cggatcgatc cggatgcgcc
     1021 gctgttctac ctcgataccg actttctatt tccggaaacg ttcgacgtgc gcgaccgcat
     1081 tgtggcccga tatggtctga aaccggcgca agtgattcag atgaaatcgc tcttgacgcc
     1141 ggagcagcag gctgcccaac atggagcggc cttatgggcc agcaagcccg atcaatgctg
     1201 cgagttgaga aaaatcgagc cattgacccg tattctcggt cagtacagcg cgtggatcac
     1261 cggaatcagg cgggatcagg ctccgacccg ggccaacgcc ggactgatcg agtgggacaa
     1321 gaaatttcag ctcgtcaaag tgaacccgtt ggccgcctgg agtacggagc aggtgtggac
     1381 gtatattcag ctccacgagg tgccgtacaa catcttgcac gaccgcaatt atcccagcat
     1441 cggttgcacg cactgcaccg cgccagtcct tcccggggac gatccgcgtt ccggtcggtg
     1501 gaaaaatttc ggcaagaccg aatgcggatt acataaataa catcatcgtc atcctgatac
     1561 gagacaaggt taataccatc acatgcaaca tctgctcgcg aaagtcgcca aaggtcaaaa
     1621 gacctccaag gatctcacct gggaagaagc caagcaggcc atgcgcctga tgatcgaagg
     1681 aactgctaca caggctcaaa tcggcgcgtt tctgacggcc atgcggttca agtccgagtc
     1741 ggtcacggaa ttggctgcct tcaccgccac cgccagacaa tatgttcctc cggtgccggt
     1801 gcgctccgga ctgggtgtcg tggatgtgcc cgtctatgcg ggaaagcggg aaacgtttca
     1861 cgccattgtc cctgccgcga tcattgcggc tgcagccggc gccgtggtgt tgctgcacgg
     1921 tgtggatggc cccgctgatc gccgcggcgt gtcctcggtc ttaaagctcc tcggtattcc
     1981 gactgatcag acggccaagc tcgtcgggcc tgaattggaa aagaaggggt ttgcctatct
     2041 ggatctggcg ctgtatcacc cgccggtgag ccggtttctg gaaatgcggc aggaattggg
     2101 tgtgcgcaat ttcttccacc cggtggcgag aatgctgaat ccggcccgtg ccgcttccca
     2161 ggtgatcggc ttgtcgcatc cgccgtattt cgagaaaact atcgaggcct tgcggatgtt
     2221 gagttgtccg cgcgcgctgg tgatccgtgg cgtcgaagga gacccggaac tttcgatcgg
     2281 taactcaacc cgtctcctcg aactcaagga cgaacgcatc acgcccttta cgtttcaacc
     2341 taaagacgcc gggctggcca tggcgacgtt tcgcgagatg gcaggtttcc cggcagagca
     2401 gcgcgaacgg gaagcggacc tgatcaagcg attggtcgct aacgagatcc agggcgggca
     2461 acgagactgg gtcctactca atgccgccat gttgctgtat gcggctggaa aggggacgtc
     2521 gatcaccggg aatctggcgg ccgcccgcaa tgcgttggag tccggtcgag caaaagccaa
     2581 actgtcagaa ctgatcgcgg ggccggggtc cgaaacagca gcggggtcga aggtcggcct
     2641 gtccgcataa atacacgagt gtgaatcatg aaacatttgc gtcaacttga agaccaaagc
     2701 gtctatattc ttcgcgaagc ctataagcac ttcaaccacc tcgccatgct ctggtcgatg
     2761 gggaaggatt caaccgtctt gctgtggctc gctcgcaagg cctttttcgg gcatgtgccc
     2821 tttcccttgc tccatgtgga tacgagttac aagattccgg caatgatcga ataccgtgac
     2881 cggattgcgc gcgaatggcg gctcaatctg gtggtgggcc agaacaagga agcgttggcc
     2941 gcgggaatga atcatacgtt ggggcgggat gtctgctgta cggcgttgaa gacaaacgcc
     3001 atgaagaatc tcgttgagga aaaaggctat accggcatta ttctcggtgt tcgtgcagac
     3061 gaagacagca ccagggcgaa agagcgatac ttttctccgc gtgacaaaca cggtgattgg
     3121 gattttcgcg atcagccgcc tgaattgtgg gatcagttca aaaccacgtt ccctccgggg
     3181 acacatatcc ggattcatcc ccttctcgat tggaccgaga tcaatatctg ggaatatatc
     3241 aagcacgaga acattccctt catggatctg tatctcgaca ggggcgatgg cacacgctat
     3301 cgcagcctcg gctgtgcccc ctgcaccatg cccatcaagt cgaccgcgaa gacggttgac
     3361 gagatcattg tagagctgcg caagagcacg gtggctgaac gggccggccg tgcgcaggat
     3421 gcggggcgag gcatggagat gctgcgtaag aagggctaca tgtaagtccg gtttccgccg
     3481 tccggctgcc ggtatcagac gaaattgggc aatgagagtt ccattgcatg agtaaccata
     3541 tgacacagca cgaccagacc aaaccgcaag aaagcctcaa tatcgtcatc gtcggccacg
     3601 tcgatcacgg gaaatctacg ctggtagggc gcctctacgc cgacaccggt tcattgccag
     3661 aaggcaagtt ggaaaaggtg caggcgatct gccggcaaca gggcaaggag ttcgagtacg
     3721 catttctgtt tgatgcgttt ctggaagagc aggaacaggg catcacgatc gatacggcgc
     3781 gcacgttctt tatctggaac gggcggcaat acatcatcat tgatgcgccg gggcataagg
     3841 aatttttaaa gaatatgatc tcgggcgccg cccgggccga agccgccctc ttgctgatcg
     3901 atgccctaga gggtgtgcgt gaacaatcga agaagcatgg atacctgcta tcgctgttgg
     3961 gcgtgaccca attcgcggtc gtcgtcaata agatggacct ggtcggttat cgacaggatg
     4021 tgttcgacgg tattgaaaaa gaatatcggg aattcctggg gcagttcagg gcggtaccgc
     4081 aacaaatgat tccggtcagt gccaagatgg gtgacaacat tgcgacgcgc agtacccata
     4141 tgaactggta tcaaggcccc accgtcttgg agaccctggc gctcttcaag aaagagcagg
     4201 ttcgttcaga gcaaccgctc cgctttcctc tccaggatgt ttacaagttt gatgcacgaa
     4261 gaattctcgc cggacgcatt acggcgggtc ggttgaaggt cggcgatcag ctcgtgtttt
     4321 ctccctccaa taaaaccgcg gtggtgcaat ccatcgaagg gttcaatgtc gatccgcctc
     4381 cgagcgaggc gcaggccggc caatcggtcg gcattacact cgacgaacag atctttgtgg
     4441 agcgtggaga gatcgcgtcg catcgggatt cggtgccgct ggtctcgaca gcggtccgtg
     4501 tgaatttgtt ctggatgggc aggcggcctc tggaaaaggg ccggaaatat gtgttgcgtt
     4561 tggccactcg ggaggtggcc tgcgaggtgg cagtcatcca tcgcattatc gacactgctg
     4621 acctggcgca gctgcaggag agccaatcgg tggccagaaa tcaggtggct gaattgaccc
     4681 tgcgggtgaa atctccgctc gcctttgacc tttcgtcctc gttcgaatcc acaggccgtt
     4741 tcgtgctggt ggacgaatat gacatttccg gaggcggcat catcaccgaa ttggtgcacg
     4801 acgaacagga aggtctacgt gaagaggcac gtcagcggga atatgcctgg ctcacgggtg
     4861 acgtcaggcc agaagaccgg gcgctgcagt acggccaccg tgcggccatt gtgctcttta
     4921 ccggctcggc ccagaccggc aagacgtttc tcgcgcggcg ggtggaagca ttgttggtcg
     4981 cggatagtcg gcatgcctat ttattggaag gtgaaaatct gctgcagggg ttggatgcgg
     5041 atttatcggc cgcggatccg tcgtttgcgt ccgagcgggt tcgtcgatat ggcgaagtcg
     5101 cgcggttgtt gatcgacacg gggctgattg tggtctcgac gagcaaaaca ttcggcatca
     5161 attatcaacg catggccgag atgatacgaa ctttggttca gccggcaccg gtgattgccg
     5221 tgcacatgag cagagccggg gaagagcctc cgcccaatac cgacttgcat tttgccgggc
     5281 cgcaggattt cgatgccgcc gcacgccaaa tcattgaaga attgaagaag cgaggcgtgc
     5341 tgatccagcc gtccgggacc aaatcgaaca ttcagtattc gatctgacga aggcgcctgg
     5401 tggcggtttg actccaccga aacccctgtg gtagcgtcct tcatcaacaa tttcgagggt
     5461 ttttcatgac catggctgcc gggaaggatc ttaagtcgat cctgacaaaa ctccagtatt
     5521 ccgacgacgc caaagtcgtg caacagatca ccgcgcagat gaagcaggtg caggctcgca
     5581 tggccggcat caagcagaaa ctggtggtga tgagcggcaa aggcggagtc gggaaaagca
     5641 tgacaaccgt caatctcgcc ctggcgtttg cgcgccaggg ggcgaaggtt ggattgctcg
     5701 atgtcgattt gaacggccct tgcgttccgc ggatgatggg tctgcatggc cagtcgctga
     5761 ccatgactcc ggaaggagcc caaccccctg tcgggccgct cggcatcaag gtcgcgtcga
     5821 tggatttttt cctgggtgac gcctcgcctg ttcgctggaa gggccccatg gacttgagtc
     5881 cggtctggct gggcttgatg gaaatgaacg tgattcggga gttccttgct gacgtggtgt
     5941 ggggtgagct ggattatctt ctggctgact tgccgcctgg agcggctgcg gataagccac
     6001 cggtcatcgc agggtttatt cccgacctgg ccggtgcgat cgtggtcacg acgccttcag
     6061 aagttgcctc cgatgtggtg caaaaatcag tcacctacgc cagggatatg ggtatccggg
     6121 tgttgggtat cgtagagaat atgagcgaat atcggtgccc gtcctgcggc gctgaaaacg
     6181 agctgtttga aggaaatacc gaggcgatgt gcgaggtgtt ggatctgccc ttgctgggtc
     6241 gtattccctt tgatcgcacg ctcgcgaaaa cgttcgataa ggggcagccg ctccttgatc
     6301 ccgactatcc gacgatccgg aaatatcaag agattgtcgg gcggattcaa tcgttgctgg
     6361 attacaaaaa agtcctggcg gaaaagctgt aagc
//
LOCUS       DAOQEW010000204         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4595_length_6341_cov_7.585428, whole genome shotgun sequence.
ACCESSION   DAOQEW010000204 DAOQEW010000000
VERSION     DAOQEW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_6341_cov_7.585428"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLS35_16535"
     CDS             complement(<1..222)
                     /locus_tag="PLS35_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20329.1"
                     /translation="MSTAPVPSHDKPAQVRRGILFIISAPSGSGKTTLCKQITANVPG
                     LWHSVSYTTRKPRPGEIDGQDYHFLDEASF"
     gene            complement(253..1131)
                     /locus_tag="PLS35_16540"
     CDS             complement(253..1131)
                     /locus_tag="PLS35_16540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNI20330.1"
                     /translation="MIRSMTGYGKKDGTSQQATVTVEIRSVNHRFLEVAVRVPRSLAQ
                     LEDPIRKAVQQRCLRGRVDVSVSVQTAGGSLKTVHIDQVLAKQYHTALKKLQKTLGLR
                     GGVDLSMVAGFRDIVSLTDEPVDTDHLGKTLLRVLGGALTELEKMRKREGEALTKDLT
                     LHLDAIREAQSIVAKKAPDLAKIAFDRMKARIETLLQSEVPDPARLQQELAVYADRSD
                     ISEELVRLESHMVQFDQQLRSQESVGKTLEFLLQEMGREVNTIGSKANDAEIAALVVR
                     MKAELEKLREQVQNVE"
     gene            complement(1128..1799)
                     /gene="nth"
                     /locus_tag="PLS35_16545"
     CDS             complement(1128..1799)
                     /gene="nth"
                     /locus_tag="PLS35_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNI20331.1"
                     /translation="MKAATAHHVSAQPDKRRLARMLRALRATEPARKVELDYRTPWEL
                     LVATILSAQCTDQRVNQVTPALFRRYRRPQDYAAADAAELEALIRPTGFFKTKAKNLI
                     HCAKAVVEIFHGQVPETMEALTGLPGVGRKTANVLLGNAFDKPAIVVDTHVKRVAGRL
                     HLTRHTDPEKVEMDLQQLLPAAEWTEGSQRLLLHGRYVCLARTPKCGNCAIYTDCHWE
                     GKLTR"
     gene            complement(1834..3603)
                     /gene="hflX"
                     /locus_tag="PLS35_16550"
     CDS             complement(1834..3603)
                     /gene="hflX"
                     /locus_tag="PLS35_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNI20332.1"
                     /translation="MRCRPGRGAGSSGAPPNVLIARSPVIATIHGHVSGLKASHLAAL
                     ERLYRRRIPASVVLTAELARTTTQLSREIRRPIGLLITRRGAIEQVLVGAGCAPTFES
                     LAKFRVGSHSLRGLRLIRTHLHDEPLSQDDLTHLALLRLDLIGALGVTETGEPGLLHL
                     AHLLPPNQQGEVCRLLKPVPFHELELQLDTFLHTLESDLQRSDTQHTVTGGQESAILV
                     SAAPKGHAEQDEHLEELTELAESAGVRVLDRIAQRTHEGYERYLLGKGKLKDVVMRSL
                     QKGADLIIFDQDLAPAQARAISEVTDLKVIDRTQLILDIFARRAHTREGKVQVELAQL
                     RYLLPRLSGHGTSLSRLGGGIGTRGPGETKLETDRRRIRDRIAHLEREIDHVARHQDQ
                     RRARRVRQGLPILSIVGYTNAGKSTLLNALTQSQISAQDRLFETLDTTSRRLRFPHDR
                     EVIVTDTVGFIRDLPKDLVGAFRTTLDELRDADMLLHVLDASASNLDQQIAAVDAVLQ
                     SLQLDTIPRVMVLNKCDRLSPREADVLSQRYHAIAISALDRETLRPLIAHLEALLPAV
                     PSPPSHEADTELPPQERALASHS"
     gene            complement(3554..4624)
                     /gene="tsaD"
                     /locus_tag="PLS35_16555"
     CDS             complement(3554..4624)
                     /gene="tsaD"
                     /locus_tag="PLS35_16555"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNI20333.1"
                     /translation="MTHAASATILGPILGIETSCDETAAAVLDGEGHVLSNIISSQQA
                     VHERFGGVVPELASRAHIQNIDQVTGRALEEAGLGWRDLRAIAVTQGPGLAGALLVGL
                     SYAKSTAYALDIPLVGVSHLEGHIASAWINRPAFPRDCVVLIASGGHTHLFHAEEKGG
                     FHLMGRTIDDAAGEAFDKGAQMLGLGYPGGPLIDAAARKGRPTAVRFPRSRVRTGQFD
                     FSFSGLKTALLYHLQGMSLEAIAEQQTDLAAAYQEAIVDVLVRQAFAAVERAGVSALA
                     VVGGVSANSRLRARLAERAVKEGIELSLPALSYCTDNAAMIAAAGQRALLQGNVASFD
                     CEAMPDCALPAWSGSGVVRRTS"
     gene            complement(4873..>6341)
                     /locus_tag="PLS35_16560"
     CDS             complement(4873..>6341)
                     /locus_tag="PLS35_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNI20334.1"
                     /translation="EKDGALKRRFQPIHVQPPSTDETVRIIQGLRDRYEEHHGVEITE
                     DAIVEAVKLADRYITDRFLPDKAIDLIDETGSRAKLQTYALPTELKALEQELKKISRE
                     KELAISMQNFEEAVRHREEEERLRKLLDESKREWKKNQEKHKPTIGKEEVAYVVSKMT
                     GIPLFKLEEEESNKLLRMEEFLHKRVIGQNEAISAVARAIRRSRAGLKEAKKPIGSFI
                     FLGPTGVGKTELARTLAEFLFNSEDALIRIDMSEYQEKFTSSRLFGAPPGYVGYEEGG
                     QLTEKVRRRPYSVVLFDEIEKAHPDVFNVLLQVLDDGVLTDSLGRKVDFKNTVVIMTS
                     NIGTKLIQKGVSLGFQNDEKSGQALRKKDEVMGELRRSFSPEFLNRIDEIVVFHSLEK
                     EHLIHILDILLHELNLRLSDKSVNIEVDDEVKQWLIKEGYEPLYGARPMRRIIQRAIG
                     DPLSEELIKGRFKDNRKIKVVLRDGAPAFIEQEAMAGV"
BASE COUNT         1202 a         1827 c         1901 g         1411 t
ORIGIN      
        1 gaacgacgct tcgtccagga aatggtaatc ctgcccatcg atttcacccg gcctcggctt
       61 acgggtcgtg taggaaacgg aatgccacaa tcccggcaca ttggccgtga tctgtttgca
      121 cagcgtcgtt ttcccgcttc ctgacggggc ggaaatgatg aagagaatgc cccgccgtac
      181 ttgagccggt ttgtcgtgag atggaacggg cgcagtgctc atagatgatt caatgccggc
      241 gctgcgccga cgttactcca cattttgaac ttgttcgcgc aatttttcca gttcggcttt
      301 catgcggacg accagcgcgg caatctccgc atcgttggct ttcgagccga tggtgttcac
      361 ttcgcgtccc atttcctgca ggagaaattc cagcgttttc cccacggact cctggctacg
      421 gagctgctgg tcgaactgta ccatatgtga ctcaagtctg accaattctt ccgagatgtc
      481 cgaccgatcg gcatacaccg ccagttcctg ctggagacgg gccggatccg ggacttcgct
      541 ttggagaagc gtctcgatcc gcgctttcat gcgatcaaag gcgattttcg cgagatccgg
      601 cgccttcttt gccacgatgg actgtgcctc ccggattgcg tccaggtgaa gggtcaggtc
      661 tttcgtcagc gcctcacctt cccgcttgcg catcttctcc agctcggtca gggctccgcc
      721 gaggacgcgc aacagggtct tgccaagatg gtcggtatcc accggttcat cggtaaggga
      781 gacgatgtcg cgaaaccccg cgaccatgga cagatccacc ccgccccgca atccgagcgt
      841 cttctgcagc tttttgaggg ccgtatggta ctgcttggcc agtacctgat cgatgtggac
      901 ggttttcagg ctgccccccg cggtctggac tgacacggag acatcgactc ttccgcggag
      961 gcagcgttgt tgaacggcct tgcgaatggg atcttccaac tgcgccaggg atcgaggaac
     1021 cctgaccgcc acttccagaa accggtggtt gaccgagcga atctcgacgg tgacggtggc
     1081 ctgttgcgac gtgccatcct ttttcccata tccggtcata cttcggatca tcgtgtgagt
     1141 ttcccttccc aatgacaatc ggtgtagatg gcgcagtttc cgcatttcgg ggtgcgagcc
     1201 aggcagacgt atcgcccatg cagaaggaga cgctgcgatc cttccgtcca ttccgctgcg
     1261 ggcagcaact gctggaggtc catttcgact ttttcgggat ctgtatggcg agtgaggtgc
     1321 aaccgccccg ccactcgttt cacgtgagta tcgaccacga tcgccggctt atcgaacgca
     1381 ttccccagca gaacgttcgc ggttttacgc ccgactccgg ggagtcccgt gagcgcttcc
     1441 atggtttccg gaacctggcc atgaaatatt tcgaccacgg cttttgcaca gtggatgagg
     1501 ttcttggcct tcgtcttaaa aaatcccgtc ggccgaatca gcgcttctaa ctccgcagcg
     1561 tcagcagcgg catagtcttg cggacgtcga taccggcgaa agagcgccgg ggtgacctgg
     1621 ttcacgcgct ggtcggtgca ttgagccgag agaatagtgg cgaccagcaa ctcccacggt
     1681 gtgcgatagt ccaactcgac tttccgggca ggctcggtgg cccgtaacgc gcggagcatt
     1741 cgcgccaagc ggcgtttgtc gggctgagcg gacacgtgat gtgcggtggc agctttcatg
     1801 atgcgcgggt tgtggcggcc aattgtgcag tggtcacgag tgagatgcaa gggcccgctc
     1861 ttgcggtggc agctcggtat ctgcctcgtg cgaagggggg ctgggaacgg ccggcaacaa
     1921 tgcttcgagg tgtgcgatca aaggccggag cgtttcccgg tccagtgccg agatagcaat
     1981 ggcatgatac cgttggctga ggacatcagc ctcacgcggt gagagtcgat cacatttgtt
     2041 taacaccatg acgcgtggaa tcgtgtccaa ttggagcgat tgcagcacgg catcaactgc
     2101 cgcaatctgc tggtcgagat tcgatgcgga ggcatcgagg acgtggagca gcatgtcggc
     2161 atcgcgcagc tcatccaatg tggtgcgaaa ggcaccgacc agatctttgg gaagatcccg
     2221 gataaaccct accgtatcgg tcacaatgac ttcgcgatca tgcggaaaac gcaggcggcg
     2281 gctggtggta tccagcgttt cgaacaggcg gtcctgcgct gaaatctgac tctgggtcaa
     2341 ggcgttgagc agggtcgatt tgccggcgtt cgtatacccg acgatggaga gaatcgggag
     2401 cccctgccgg acacgtctcg cgcgccgctg atcctgatgg cgagccacgt ggtcgatctc
     2461 gcgctcgaga tgggcgatac ggtcgcggat gcgccggcga tcggtttcca gtttggtttc
     2521 gccgggaccg cgtgtgccga ttccgccgcc gagccgtgac aatgaggtac catgccctga
     2581 gagccgcggc aacaggtacc ggagctgggc caactcgact tgcactttgc cttctcgcgt
     2641 gtgcgcgcgc cgggcaaaaa tatccagaat gagctgggtg cgatcgatga ctttcaggtc
     2701 ggtcacttcg gagatcgctc tggcctgtgc cggcgccagg tcctgatcga agatgatgag
     2761 gtcggcgccc ttctgcagcg accgcatgac gacgtctttc aatttgccct tgccgagtaa
     2821 ataccgctca tacccctcat gggtgcgttg cgcaatacga tccaggactc gtacgccggc
     2881 tgattcagcg agttccgtca actcttctaa atgctcgtct tgttcggcat ggcctttcgg
     2941 cgcggcgctc accaaaatgg ctgattcctg gcctccggtg acggtgtgct gcgtatccga
     3001 tcgctggagg tcgctttcaa gcgtatggag gaaggtatcc agttgcagtt ccagttcatg
     3061 gaatggcacc ggcttcagga gacggcacac ctcgccttgt tgattgggcg gaagtaggtg
     3121 cgcgagatgc agcaggccgg gttcgcccgt ctcggtcaca cccaacgctc cgatgagatc
     3181 caatcgcagg agtgctaagt gtgtgagatc atcctggctc agcggttcgt catgaagatg
     3241 ggtgcggatc agtctgaggc cacgcaatga atgtgatccg accctgaatt tggccagcga
     3301 ctcgaacgtc ggtgcacagc ctgcccccac caggacttgc tcaatggctc cacggcgcgt
     3361 gatcaggagt ccaatgggcc ggcgaatttc acgtgacagc tgggttgttg tacgagccaa
     3421 ttcggcagtg aggactacag aggcagggat gcggcggcga tacaatcgtt ccagcgcggc
     3481 aaggtggctg gctttcaggc cgctgacatg tccgtgaatc gtggcgatga cgggactcct
     3541 tgcgatgaga acattaggag gtgcgccgga cgaccccgct ccccgaccag gccggcagcg
     3601 cacagtccgg catggcttcg cagtcgaatg acgcgacatt cccctggagg agcgctcgtt
     3661 gtcctgcagc ggcaatcatc gccgcattat cagtgcagta tgagagggcc ggcaggctta
     3721 attcaatgcc ttccttgacg gctcgctcgg ccaatcgggc tcgaagccgc gaatttgcgg
     3781 agacaccgcc cacgacggca agggcggaca cccctgcccg ctctacggcg gcgaaggcct
     3841 ggcgaaccag gacatccacg attgcctcct ggtaggccgc agccaggtcg gtctgttgct
     3901 cggcaatggc ctccaggctc atgccttgta aatgataaag cagcgcggtt ttgagtccgc
     3961 tgaaactaaa atcgaactga ccggttctca cgcgagagcg cggaaatcgc acggctgtgg
     4021 gacggccttt ccgcgccgcg gcatcgatga gcggtccgcc ggggtacccg agacccagca
     4081 tctgcgcccc cttatcaaag gcctcgcctg ccgcatcatc gatggtccgt cccatgagat
     4141 gaaatccacc cttctcttcc gcgtggaaca gatgcgtatg tccccccgac gcaatgagga
     4201 ctacgcagtc tcttggaaat gccggacgat tgatccaggc cgacgcaata tgcccctcga
     4261 ggtgactgac gccgaccagc ggaatatcca aggcataggc tgtggacttc gcataactga
     4321 ggccgaccag aagggcgcct gccagccctg gcccctgagt gacggcaatg gcccgcagat
     4381 cgcgccatcc gagaccggct tcctcgagtg cccggcctgt cacctggtcg atgttctgga
     4441 tatgagctcg cgaggcgagt tccggcacca ctccgccgaa tcgctcatgc acggcttgtt
     4501 gagatgaaat aatgttggaa aggacgtgtc cctctccatc gagaacggcg gcggcagttt
     4561 cgtcacagga ggtttcgatg ccgagaatgg gaccgaggat tgttgccgaa gctgcgtgag
     4621 tcatagggca gattgatacc gccgcgttgg aagagctgac cgttcgtttg cttcagagga
     4681 ccgcgccagg gggtgtggac acgtggctgt cacccttcgg gcgagagacg gtttggggtt
     4741 ggatgaattc aacaattgtc tccgccgtga aaagcgacaa cccccggatc cggttgggga
     4801 ccgcgggggt tgccgggtag cgtacaggat ctcaagtacc aatcgatcct atcgtgataa
     4861 gcgcgtcgtg ttctacacgc ccgccatggc ttcctgttca ataaaggccg gcgcgccgtc
     4921 gcgcagaacg accttgatct tccggttatc cttgaagcgg cccttgatga gctcctcgga
     4981 caacgggtcg ccgatcgctc gctgaatgat gcggcgcatg gggcgcgcac cgtacagcgg
     5041 ttcataccct tccttgatca gccactgttt gacttcgtcg tccacctcga tgttgacgct
     5101 cttgtcgctg aggcggagat tcagctcgtg cagcagaata tccaggatat ggatgaggtg
     5161 ttccttctcg agcgagtgga aaaccacgat ctcgtcgata cgattcagga attccgggct
     5221 gaaggatcgg cggagctcgc ccatcacttc atccttcttg cgtaacgcct gaccgctctt
     5281 ttcgtcgttc tggaaaccga gcgagacgcc cttttgaatc aacttcgtac cgatgttgga
     5341 cgtcatgatg acgaccgtgt tcttgaagtc gacttttctg cccaggctgt cggtgaggac
     5401 gccgtcgtcc aacacctgca acaggacgtt gaacacgtcc gggtgggctt tttcgatttc
     5461 gtcgaacagc accacggaat aagggcgccg acgaactttc tcggtcagct ggccgccttc
     5521 ttcatatccg acataaccgg gaggggctcc gaagagccgc gaactggtga atttctcctg
     5581 gtattcggac atatcgatcc ggatgagcgc atcttcacta ttgaagagga actccgccag
     5641 ggttcgcgcc agttcggttt ttccgacacc ggtcggtccg aggaagataa aggagccgat
     5701 aggcttcttc gcttccttga gtccggcccg ggatcggcga atggctcgtg cgaccgccga
     5761 gatggcttcg ttctgcccga tgacccgctt gtggaggaat tcttccatac ggagcagttt
     5821 gttggattct tcctcttcca gcttgaagag cggaatgccg gtcattttgg acacgacata
     5881 ggcgacttct tccttgccga tggtcggttt gtgcttttcc tggttctttt tccattcgcg
     5941 cttggattcg tcgagcagtt tccgcaggcg ctcttcttct tcccgatgac gaaccgcttc
     6001 ctcgaaattc tgcatcgaga tagccagttc cttttcacgc gagatcttct tgagctcctg
     6061 ttccagcgct ttgagttcag tcggaagcgc gtaggtttgc aatttggcgc gagagccggt
     6121 ttcatcgatc agatcgatgg ccttgtctgg caagaatcgg tcagtgatat accggtctgc
     6181 caacttgacg gcttccacga tcgcgtcctc ggtgatctcg acgccatgat gttcttcgta
     6241 ccgatcgcgc aagccctgaa tgatgcggac cgtttcatcc gtactcggcg gttgcacatg
     6301 gatgggttgg aagcggcgct tgagggcccc gtccttctca a
//
LOCUS       DAOQEW010000205         6318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4626_length_6318_cov_7.123583, whole genome shotgun sequence.
ACCESSION   DAOQEW010000205 DAOQEW010000000
VERSION     DAOQEW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4626_length_6318_cov_7.123583"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..>1902)
                     /gene="ligD"
                     /locus_tag="PLS35_16565"
     CDS             complement(259..>1902)
                     /gene="ligD"
                     /locus_tag="PLS35_16565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HNI20335.1"
                     /translation="DSDLLYYVFDLLYLNGYDLREVPLLERKRLLSALLKDRPSSSLI
                     QYSDHITGRGDVAFAEACRSGMEGLIAKRADAPYLSGRNRNWVKVKCGQRQEFVIGGF
                     TDPSGSRSAFGALLLGVYDAQGRLQFSGRTGTGFTERSLKELHTRLKKIEQKQPAFVN
                     PPVGSEARGVHWVKPLLVAEVAFAEWTNEGQLRQASFQGVREDKDARSIIHERPDSGP
                     SSPKARKGRGHARSRRISSNQQTPSAGAEADETAPVIGGVTLSHPDRVLFPEQGITKI
                     ALARYYESISDWILPHVEGRPLTLVRCPEGHKKECFYQKHAGEHIPDTVGRVEIPEEK
                     GSSWYMVADDLPALIGLVQMGVLELHTWGAKRDQLERPDRMILDLDPDPAVEWPLVIE
                     GAQLLRTLLEELDLVSFVKTTGGKGLHVVVPLQRAHTWDEVKTFSKAVADHLVRAIPE
                     RFLANMSKQKRKGKIYVDYLRNARGATAVAAYSTRARPRAPVSVPLAWNELSTDLRSD
                     HFTVDNIAERLEHLRQDPWKTYFTTRQRITKNMVRRLAG"
     assembly_gap    1903..2002
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2003..2641)
                     /locus_tag="PLS35_16570"
     CDS             complement(<2003..2641)
                     /locus_tag="PLS35_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HNI20336.1"
                     /translation="MSLRQYWKKRNFRNTPEPRGKETPAGARLQFVIQQHAASRLHYD
                     FRLELDGTLKSWAVPKGPSLDPAQKRLAVHVEDHPLDYASFEGIIPEKQYGAGTVLLW
                     DRGYWEPIGDPKADYRRGRLKFTLHGEKLQGIWNLVRMGGREEAGKENWLLIKEKDAE
                     AKRGTQSRVTDRLTSSVASGRSMEQIATGEHAVWHSNRSSTTSAKKRAVSRPV"
     gene            complement(2675..3415)
                     /locus_tag="PLS35_16575"
     CDS             complement(2675..3415)
                     /locus_tag="PLS35_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNI20337.1"
                     /translation="MMPTSWLPACHTIFKKRYWATAALFLSAWLLSGCAQSAHQSGRD
                     LLDQILPDQLQQQIDPTVTFPELKANPSAYQGKTIMVSGIVVKSKRRKDQTEIEVLQI
                     PTGTGAVPSKDRAHSQGRFLAVKSGEFLDPAVVDAGTPVTVVGEVGPPVTRPMDDGEY
                     TYPVLAITHLVDWDEVNPAMRSAPYAYPYGRSMYAYPYAYWGPGGWYGGYPGYGYGYP
                     YYGFYGFGGGSSAPAGPPPADAPPQFNK"
     gene            complement(3372..4007)
                     /locus_tag="PLS35_16580"
     CDS             complement(3372..4007)
                     /locus_tag="PLS35_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI20338.1"
                     /translation="MTSSKTLIAVVLICLIGTGMLNSRPVLGEWYVAGQFGANFADQL
                     SDIHGTGNLSGFRAPDFDLQNSLLYGAKVGNFPGNGWFGLELDVFHSTPHVKNLDDVP
                     GFHMRVTNVGVNLLARYPGVTFQPYAGIGAGVAIARIGDSATTRGSTDASSALNLLVG
                     IRAFVTPYVALFTEYKYSQSTFEFSHAFGPSSGFNGNYDAHILVTGLSYHF"
     gene            complement(4054..>6318)
                     /locus_tag="PLS35_16585"
     CDS             complement(4054..>6318)
                     /locus_tag="PLS35_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNI20339.1"
                     /translation="RSRFPNIPRRVSGYNLPALLPEHGFDVAKALVGSESTCVTVLEA
                     TVRLVSSPRVRSLLVLGYPDVYAAGDHIPDILRAKPIGLEGMDDRLVEDMTKMKIHPD
                     DVKLLPEGRGWLLAEFGGESKDEADGQAHRCMELLKAAGHPPSMKLFDRRRDEQVIWT
                     VRESGLGATAHVPGEQVTWEGWEDSAVPVERLGDYLRALRRLFEKYRYACALYGHFGQ
                     GCVHTRIDFDLQTQEGISRFRSFLFEAADLVKSFGGSLSGEHGDGQSRAELLPRMFGP
                     ELIGAFEEFKAIWDPDGKMNPGKIVRPFRVDENLRLGSDYHPPDPQTDFTFPQDEYSF
                     SRATLRCVGVGECRRQAGGTMCPSYRATREELHSTRGRARLLFEMLEGDPLTRGWRND
                     TVKEALSLCLACKGCKGDCPVHVDMATYKAEFLAHYYKGRLRPRSAYSMGLIHRWARW
                     ASRAPGMANFITQTPGLCDVVKMLGGISPHRRMPRFAAETFQAWHRQRAKRAGGGQPV
                     LLWPDTFNNYFYPETARAATEVLEAAGCAVFVPQTNLCCGRPLYDFGMLRQAKRHLQQ
                     ILCELASTIRSGMPLVVLEPSCAAVFRDELGNLFPHDEDARRLAKQTFTLAEFIDKRL
                     PDWELPQSHQSVTVHGHCHQKAVIGLGAEERVLDRLGLNVQMLDTGCCGMAGSFGFKQ
                     EHYDISMQIGELALLPAIRNTATDTIVISDGFSCRQQIEQSTHRQGMHLAEVIHQAMG
                     NVTMHVSASAHGGT"
BASE COUNT         1322 a         1926 c         1661 g         1309 t
ORIGIN      
        1 gaccaagaat ccgccgcagc ggaggcatcg gcaggtatcg tgttgagaaa caacaggagt
       61 agatctggtt ttgccgggga gaggaaggct tctcgtcgtg gtggcattca tacggttctc
      121 ctttcgtcgt gatcctgata ggcttcggcg gaatgtcttc caccgattgt catgatcact
      181 gataacagtc ccgtacgatt cactctacta gcactccccc caggtcccct agcaaagcac
      241 cctggcggaa cttcgacatt atcctgccag ccggcgcacc atattcttcg taatacgttg
      301 cctcgtggtg aagtaggtct tccatggatc ctgtcgcaaa tgctcgagtc tttcggcaat
      361 attgtcgact gtgaaatggt cagaacggag atcggtcgac aattcattcc aggcgagcgg
      421 aaccgagacg ggcgcgcgag gccgcgcgcg agttgaatag gcggccaccg ccgtggcccc
      481 tcgggcgtta cgcaagtagt cgacgtagat cttgcccttg cgtttctgtt tcgacatgtt
      541 ggcgagaaat cgttccggaa tcgcgcggac gagatgatca gccacggctt tggagaaggt
      601 ctttacctca tcccacgtat gggcgcgttg caacgggacg acgacatgca gccccttgcc
      661 tcctgtcgtc ttcacgaatg agacgaggtc cagctcctcg agcaaggtgc gcagcaattg
      721 cgcaccttca atcaccaatg gccattcgac cgcaggatcg ggatcaagat ccagaatcat
      781 ccggtccggc cgttcgagct ggtctcgctt tgccccccag gtatgaagtt ccaatacccc
      841 catctgcacc agcccaatca gcgcgggaag atcatccgcc accatgtacc aggacgagcc
      901 tttttcttcc gggatctcga cccggcccac agtgtcgggt atgtgctccc ctgcgtgctt
      961 ctgataaaag cactccttct tgtgcccctc cggacagcgg accagggtca acggccgacc
     1021 ttcgacatgc ggcagaatcc aatcgctgat ggactcgtaa taccgagcca gcgcaatttt
     1081 tgtgatgcct tgctcgggaa acaacacccg atccgggtga ctgagcgtga caccgccaat
     1141 gaccggcgcg gtttcatccg cttcggcacc ggcagaagga gtctgctgat ttgatgagat
     1201 ccggcgcgag cgagcatgtc cccgcccctt gcgtgccttc ggagaagaag ggcctgagtc
     1261 aggtcgttca tgaatgatgg aacgggcgtc tttgtcttct cgtacacctt gaaatgaagc
     1321 ctggcgcagc tgcccctcgt tggtccattc ggcaaaggct acttcagcaa ccaggagagg
     1381 tttgacccaa tgcacaccac gcgcctcact gccgaccggc ggattcacga atgccggctg
     1441 tttctgctcg atcttcttca agcgcgtgtg taattccttg agggagcgct ccgtaaatcc
     1501 cgtcccagtg cgtccggaaa attgcagccg cccctgcgca tcataaacac caagaagcag
     1561 ggcaccgaag gcgctccgtg aaccggaggg gtcagtaaat cctccgatca cgaattcctg
     1621 ccgctgcccg catttaacct tgacccaatt gcggtttctg cccgaaagat acggagcatc
     1681 ggctcgtttg gcgatcaatc cctccatccc gctccggcag gcctcggcga atgcgacgtc
     1741 gcccctgccg gtaatatgat cgctgtactg aatcaaggaa gacgacggtc ggtctttcag
     1801 tagagccgac agaagccgct tgcgctccag gagcggcacc tcccgcagat cgtagccatt
     1861 cagatagagc aggtcgaaca cgtaatagag cagatcactg tcnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nntaccgggc gggagacggc acgtttcttt gccgatgtgg
     2041 tcgaggaccg gttcgaatgc cacaccgcat gctctccggt ggcaatttgc tccatactcc
     2101 tgccgctggc gacgctactc gtcaaacggt cagtcacccg gctctgcgtc ccgcgcttgg
     2161 cttcagcgtc cttctctttt atcagcagcc aattctcttt ccctgcctct tcgcggccgc
     2221 ccatgcgtac cagattccag atcccctgca gcttttcccc gtgtaaggtg aatttgagtc
     2281 ggcctcgccg atagtctgct ttcggatcac cgatcggttc ccagtagccc cgatcccaga
     2341 gcaacaccgt gcccgcgcca tactgtttct ccggaatgat gccttcgaag gacgcatagt
     2401 ccagtggatg gtcctcgaca tggaccgcca gccgcttctg tgccggatcc agactcggcc
     2461 ctttgggaac cgcccaactt ttcaatgtgc catccaactc caggcgaaag tcgtaatgga
     2521 gccggcttgc ggcgtgttgt tgaatgacaa actggagccg ggctcccgcc ggcgtttctt
     2581 ttcctcgcgg ctcgggagtg ttgcggaagt ttcgtttctt ccagtattga cgaagactca
     2641 tgtcactcct gcgaagggat ggtcgattgc cccatcactt attgaactgc ggaggagcat
     2701 ctgccggtgg cgggccggcc ggcgcggagg atcctccacc gaagccatag aaaccgtaat
     2761 agggatatcc gtacccatac ccaggatagc ccccgtacca gccccccggc ccccaatagg
     2821 catagggata ggcatacatc gagcgtccat acggataggc atacggggcg gaacgcatag
     2881 ctggattaac ttcatcccaa tcaacgagat gtgtgatggc gagaacggga taggtgtatt
     2941 ccccgtcgtc catcggcctg gtgaccggcg gtcccacttc accgacgacc gtcaccggcg
     3001 tccctgcgtc gactacggct gggtcgagaa attcaccgct ctttacggca agaaatcttc
     3061 cctgagagtg cgcacgatcc ttgctgggca cagccccggt tcctgtcggg atctgcaaga
     3121 cctcaatctc cgtctgatcc ttcctccgct tactcttcac cacaatgcca ctgaccatga
     3181 tcgttttgcc ctgataggcg gaaggattgg ctttcagctc tggaaacgtg acagtgggat
     3241 caatctgttg ctgcagctga tccggcagaa tctgatccag cagatcgcgc ccggactgat
     3301 gcgcactttg cgcgcacccg ctgagaagcc acgcagacag gaagagcgct gctgtggccc
     3361 aatagcgttt cttaaaaatg gtatgacaag ccggtaacca agatgtgggc atcataattt
     3421 ccattaaacc cactcgaagg gccgaacgcg tgactgaatt caaaggtgga ttggctgtat
     3481 ttgtattcgg taaacagggc cacatatggc gtcacgaatg ccctgatccc gaccagtaaa
     3541 ttcaacgccg aagatgcatc ggtactgcct ctggtcgtag ccgaatcgcc gattcgcgcg
     3601 atggccactc ccgcgccgat tccagcatag ggctggaagg tcactccagg gtatcgagcc
     3661 agcagattga cgccgacgtt cgtcacccgc atgtgaaagc ccggcacatc atcgaggttc
     3721 ttgacgtgcg gcgtactgtg aaagacgtct agctccagac cgaaccatcc gtttcccgga
     3781 aagttcccga cctttgctcc atacagcagc gagttctgca aatcaaaatc cggcgcgcga
     3841 aagccgctga gattgccggt tccgtgaatg tcactcagtt gatcggcaaa attagctccg
     3901 aactgaccgg cgacatacca ttcgcctaaa accggccggc tattcagcat acccgtcccg
     3961 atcaggcaaa tgagcaccac cgcgatcaat gtcttcgagg acgtcatcga gcaatcactt
     4021 ccactctgta tcggcacggc ccctgattca tggttaagtc ccaccgtgag ccgaggccga
     4081 cacgtgcatt gttacgttgc ccattgcctg atgaatcact tcagccaaat gcatcccctg
     4141 ccggtgcgtc gattgctcga tctgttgtct gcagctgaag ccatccgaga tcacaatcgt
     4201 atctgtagcg gtatttctga tcgctgggag caaggccaat tctccaatct gcatggaaat
     4261 atcgtagtgc tcctgtttga aaccaaagga gccggccatg ccgcagcacc cggtatccag
     4321 catctgcaca ttcagcccca atcgatccag tacgcgttct tctgcaccca gcccgatcac
     4381 ggccttttgg tgacaatggc catgcaccgt cacggattga tgcgattgag gtagttccca
     4441 gtccggcaaa cgtttgtcga taaattccgc cagcgtgaag gtttgtttcg ccaggcgtct
     4501 ggcatcctcg tcatgaggga acaggttgcc caactcgtct cgaaaaacgg cagcacaact
     4561 gggctccagc accaccaacg gcataccgct acggatcgtg gacgctaatt cacacaagat
     4621 ctgctgcaag tgtcgcttgg cttgccgcag catgccgaag tcatacagcg gtcgaccgca
     4681 acagagattg gtctgcggca cgaacacggc acatccggca gcctccagca cctcggtcgc
     4741 agccctggct gtctccgggt aaaaataatt gttgaacgtg tccggccaaa gaagtaccgg
     4801 ttgcccaccg cctgctcgtt tcgcgcgctg acgatgccat gcctgaaacg tctcggctgc
     4861 aaagcgaggc attctgcggt ggggcgaaat accccccagc attttgacga cgtcgcacaa
     4921 tccaggcgtc tgggtgatga aattggccat gccgggcgcc cgcgaagccc atctagccca
     4981 ccggtggatc aatcccatgg aataggccga tcgaggacgc aaccgccctt tgtaataatg
     5041 ggcgagaaac tcggccttgt aggtcgccat gtcgacatgg acggggcaat cccccttgca
     5101 ccccttacag gccaggcaga ggctgagcgc ttctttcacc gtatcattcc gccagccgcg
     5161 cgtgagggga tcgccttcca gcatttcaaa caacaggcgt gcccgcccgc gagtcgagtg
     5221 gagttcctca cgcgtcgcgc ggtaactcgg gcacatggtg ccccctgcct gccggcgaca
     5281 ttcacccacc cccacgcaac gaagcgtcgc tcgtgaaaaa ctatattcat cctgcggaaa
     5341 ggtaaaatca gtctgaggat ccggcgggtg gtagtcgctc cccagtctca ggttttcatc
     5401 gacacggaaa ggccggacga tcttgcccgg attcatcttt ccatcgggat cccagattgc
     5461 cttgaattct tcaaacgccc ctatgagttc cggtccgaac attcgcggga gtaactcggc
     5521 gcgcgattgg ccgtcaccat gttctcctga aagggaaccg ccgaaactct tgacgagatc
     5581 ggccgcctcg aacagaaacg accggaaccg gctgatccct tcctgggtct gcagatcaaa
     5641 atcgattctc gtatgtacgc agccttgccc gaaatgtccg tacagcgcac aggcatagcg
     5701 atacttctcg aacagacggc gcaaggccct caggtaatcg cccaagcgct cgacagggac
     5761 agccgaatcc tcccaccctt cccaggtcac ctgttctcca ggcacatggg ccgtcgctcc
     5821 aagccctgat tcacggaccg tccagatgac ctgctcgtct cgccgtcgat caaacaactt
     5881 catggagggg gggtgtccgg cggccttgag gagttccata cagcgatgcg cctgcccatc
     5941 cgcctcgtcc ttgctctcac cgccgaactc cgcaagcagc cagccccgcc cttcaggcag
     6001 caacttgacg tcgtccgggt gaattttcat tttggtcatg tcctccacga gccgatcgtc
     6061 cattccttca aggccgatcg gcttcgcgcg gagaatatcg gggatatgat caccggcggc
     6121 atacacgtcc ggataaccca gtaccagcaa ggaacgtaca cgcggactgg aaaccagccg
     6181 caccgtggcc tccaacactg tcacgcaggt actttcggac cccaccaatg ctttggccac
     6241 atcgaaccca tgctcgggca gcagcgcggg aaggttgtag ccggaaaccc ggcggggaat
     6301 attcgggaag cgcgatct
//
LOCUS       DAOQEW010000206         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4789_length_6179_cov_6.663945, whole genome shotgun sequence.
ACCESSION   DAOQEW010000206 DAOQEW010000000
VERSION     DAOQEW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_6179_cov_6.663945"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLS35_16590"
     CDS             complement(<1..724)
                     /locus_tag="PLS35_16590"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HNI20340.1"
                     /translation="MWTIYTFGNGDVLTQILLGIRIMMTTGRFESLMELALLVFAMFG
                     LVYFVFNRRFLVPLVVGAFFILFVSVRITVDVSVEDQVNPDVPDQIVNSVPLAIALPA
                     YLSGQVGYYTTQLVEASFPMPVQYTQGQASFNRPLFDLQKVLNARLQDSDLARNTDSY
                     FMMCVFREVGLLQKNLGTLMNSPNLLNDIGATNNALTALGYTGGTLEAAPRVCPDFYS
                     SLILPAYTTGQPDYEGAMRHLRT"
     gene            complement(769..2103)
                     /locus_tag="PLS35_16595"
     CDS             complement(769..2103)
                     /locus_tag="PLS35_16595"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HNI20341.1"
                     /translation="MRSKLILVGVCMWAVLVWPPLVQAQSMNDALTNMFTSWGVGITS
                     PPGAYESQSRGYFSGGGVSVRLWQDPMRLWSIAPPRLSVGCQGIDVYLGSFSYGKLDR
                     YVQLLQQIGTGAVLGYAFQLAMKAICEDCADVLNKIEAAARALNAAGRIQPCQTGIEL
                     GKALAGNEASKQKLASRFGDAWQKMKEAGGAIGDVFEDRDTVKNQSNADAASALKGTE
                     YDVTGNLVWDVLTQAGLDQNLIVMVMSVTGTVIVGNDGDVQTRDPTMTFEQLVDSHLG
                     DVVKVFNCGGDSECLNPTITDATDIEGFESRVYTSMSNLIEDLYSGSAISATDQQIIN
                     MTPVPILTMLADYGRPRDVGNQIARLSSEVVAADMGYQWVKWAATETSRNVSYIQKVK
                     PEWPGNLQDFQGRLHQILRGASESLAKRQRMVNNINQLMQLTKTQGEMRSLR"
     gene            complement(2114..3016)
                     /locus_tag="PLS35_16600"
     CDS             complement(2114..3016)
                     /locus_tag="PLS35_16600"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNI20342.1"
                     /translation="METTKLLHALAWYCVTFCSVGVVSSAVLAAEPPGSQTFIDHPRQ
                     GWFSYQDPSAEAEEDAEESPVPVTMDSLPPEAWLDPSKYRTLFKGLSVEGVKLNALPM
                     TVLRELVSAKKERALDHPSVENVTTYIKVQKEAYDRSQRFTDAWQIAMYTDPKLDYAS
                     QHPTSTYGHSVEAEIKRNTEEQLLAGTADRVGLFFFFTSTCPFCQEQSKVLKVFADTY
                     GLTVKPVSLDGVGLPEYPQPATNNGMAENVGVHMVPMIYLAIPEENFLKPLGAGLMTN
                     ADLRERLLVLLRNRGLLGERRSQG"
     gene            complement(2986..4074)
                     /locus_tag="PLS35_16605"
     CDS             complement(2986..4074)
                     /locus_tag="PLS35_16605"
                     /inference="COORDINATES: protein motif:HMM:NF018536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HNI20343.1"
                     /translation="MNKLSKRRIQHMLLLVCLSCVLIGVVTLWPSGSAEAVCTTGTNV
                     LANSLATTCWECMFPISLSGVTVFNLGEDFTEPAIGPGHAPFPPQLCGCVCLGPICIP
                     GLPLGIWEPKNLVEAVHDPLCFPTLGVSLAGGTSYIGRGMATTQMDSELNYAFFHVHY
                     FVFPLWAIVGIGMDILCLNPTGIADEIDLAYASEIDPLWDDDLISLILFPEALVLANP
                     VALAACAADSVAAAVGFPIDPLFWCAGSFGFMYPPTGHVSGADGDLKPAALSMTRLLA
                     KLARAGTEFWTSANGPLLCEDIPTALIVKSQYKFQLLWPIPNTGLPPPCCQPLGRTLM
                     MWGLGKIVPAVGEDFVWLLWKRQNCCML"
     gene            complement(4067..5143)
                     /locus_tag="PLS35_16610"
     CDS             complement(4067..5143)
                     /locus_tag="PLS35_16610"
                     /inference="COORDINATES: protein motif:HMM:NF021209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC family F-type conjugative pilus assembly
                     protein"
                     /protein_id="HNI20344.1"
                     /translation="MAQDIARYSRQALRHGRPQESPKEVRRDGVYLLLSFSLPNDTLK
                     DYLREAKLLGAKVLLRGLVQDSFKVTQERIKHLLFTGDHPDDSLLVGIGIDPVIYRTV
                     GAGEVPALVFVKDEKFLIASGGASVAHLLTLLSKEQAGVTPWIEWFERRHRGFLQGGP
                     TEEAPPQVPTIGQSMKVQGDARGADIAERDMIDVMKERVAKADWPDIQRRSGDALKRQ
                     FAKGPGLALPHAEEARVVLVDPTVEYPEDIKDPTTNTVLIKSGTKINPFDKVRWIRTL
                     VFFDGTSSSQVGWVQQYLKEHDPKFVKLIISDGDVQKVMEQLHQRVYWANPLLVSRMG
                     VGAVPSIVSQLGRHLRVEEVAIHE"
     gene            complement(5318..5896)
                     /gene="lepB"
                     /locus_tag="PLS35_16615"
     CDS             complement(5318..5896)
                     /gene="lepB"
                     /locus_tag="PLS35_16615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNI20345.1"
                     /translation="MKLFNALSLLNVLKRAPTILDWLDRHRWTHVGLMLFCGAIITAG
                     VIAPWYEVAARMSGSWPHHNFFLIQKGQPVGRGDLVAFIMRRDYVERVQPGHLHRDYA
                     DVGNRWMKRIVGVPGDHIVIKEDEVYINGQSMGRGFGIDRYGQRIDLASLPEVIPEGE
                     YYVALPHPRSFDSRYYGLIRKADILGTVTPLI"
     gene            complement(5826..>6179)
                     /locus_tag="PLS35_16620"
     CDS             complement(5826..>6179)
                     /locus_tag="PLS35_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20346.1"
                     /translation="LENAEFVGLLKQKTESIAAFRNNGRIVLSEMEYRLLSSVTKTTE
                     YSEIFMITPFGRGICRLTVPRETQLLFTTNPDELSMIDRVRKEGPKELSIDEAIQCII
                     AAERAQAGADNPRLA"
BASE COUNT         1416 a         1886 c         1553 g         1324 t
ORIGIN      
        1 gtgtacgcaa atgccgcatc gccccttcgt agtcaggctg ccccgtggta tacgcaggaa
       61 ggatgagaga actatagaag tcgggacaca cccgcggggc cgcttccaac gtcccacctg
      121 tatatccgag tgccgtgagt gcgttattcg tagccccgat atcattgagc agattcggag
      181 aattcatcag cgtaccgagg ttcttctgga gcaggcccac ctcccgaaac acacacatca
      241 taaaatatga atcggtattg cgggccagat ccgagtcttg caatctcgca ttgaggactt
      301 tttgcagatc gaatagcggg cgattgaatg acgcttgtcc ctgggtatat tgcacgggca
      361 tcggaaacga cgcttcgaca agctgagtcg tgtagtaccc tacttgcccc gagaggtatg
      421 ccggtaacgc gatcgccagc ggcacactgt tcacaatttg atccggcaca tcgggattga
      481 cttggtcttc cacggacacg tcgaccgtga ttcggacact gacaaagaga atgaaaaagg
      541 ccccgacgac cagcgggacc aagaagcgcc gattgaacac aaaatacacg agtccgaaca
      601 tcgcgaagac aagaagtgcg agctccatca ggctttcgaa gcgtccggtg gtcatcatga
      661 tccggatgcc cagcagaatc tgcgtcagca catcgccgtt gccaaacgta tagatcgtcc
      721 acatgatgcg ctatcctctc cctcacatcg cagacagact tactccgact accgcagcga
      781 tcgcatttcg ccctgcgtct tcgtcaactg cataagctgg ttgatgttat tcaccatacg
      841 ttgccgcttc gcgagactct cactagcccc ccttaggatc tgatgtagcc gcccctgaaa
      901 gtcttgcaga ttgcctggcc actcgggttt gaccttttgg atatagctaa cgttcctgga
      961 cgtttcggtg gccgcccact tcacccattg ataccccatg tccgcagcga ccacttctga
     1021 agacaatcgc gcaatctgat tgccgacatc ccgtggacgc ccataatcgg cgagcatggt
     1081 cagaatgggg accggggtca tgttgatgat ttgctgatct gtcgcgctga tagctgagcc
     1141 ggagtagagg tcttcgatca ggttactcat ggacgtatac acgcgactct cgaatccttc
     1201 gatatcggtc gcgtcagtaa tcgtggggtt caaacattcc gagtcgcccc cacaattaaa
     1261 cactttcacc acgtcgccca gatgactatc gaccagctgt tcgaaggtca tcgtcggatc
     1321 gcgggtttga acatcgccat cgttcccgac tataacggtc ccggtgaccg acatcaccat
     1381 gacgatgaga ttctgatcca ggccagcctg cgtcaggaca tcccacacca gattgccagt
     1441 gacatcgtat tccgtgcctt tcaatgctga ggccgcatcc gcattcgact ggttcttgac
     1501 ggtatcccgg tcttcaaaca cgtctccaat ggcgccgccc gcttccttca tcttctgcca
     1561 ggcatcgccg aatcgactcg cgagcttttg tttggaggct tcgttcccgg ctagcgcttt
     1621 gcccaattca atccctgttt gacagggttg gatccgtccc gcagcattga gcgctctcgc
     1681 agccgcttca atcttgttga gcacgtccgc gcagtcttca caaatggctt tcatcgccag
     1741 ctggaaagcg taccccaaga cggcaccggt cccgatttgc tgcagcaatt ggacgtaccg
     1801 atctagtttg ccgtaggaaa acgagcccag gtaaacgtcg atgccctggc agcccacgct
     1861 caatcgcggc ggggcaatcg accacaaccg catcgggtcc tgccacaaac ggaccgagac
     1921 cccgccgccg ctgaagtagc ctcgactttg actttcgtag gcacctggag gactcgtgat
     1981 cccgacaccc cacgacgtga acatgttcgt aagggcatca ttcatcgact gcgcctgtac
     2041 caggggaggc cagaccagta ctgcccacat acacactccc accagaatca gtttgcttcg
     2101 catcgtgtgt cctttatccc tgagaccgtc gctcgcccaa caatcctcgg ttacgcaaca
     2161 acaccagcaa gcgctcccgt agatccgcat tcgtcatgag accggctccg agcggcttta
     2221 agaagttctc ctcaggaatt gccaaataaa tcatgggcac catatgcacg cccacattct
     2281 ccgccatgcc attgttagtg gcgggctgcg ggtactccgg caatcccacc ccgtccagcg
     2341 acacaggttt caccgtcagt ccgtaggtat cggcaaagac cttcaatacc ttggactgtt
     2401 cttgacaaaa cgggcaggtc gaggtgaaga agaagaacag tccgacccgg tcggctgtgc
     2461 ccgccagaag ctgctcttcg gtgttccgct tgatctcggc ttcgacgcta tgcccatagg
     2521 tggacgtggg gtgttgcgat gcatagtcga gtttggggtc cgtgtacatg gcgatctgcc
     2581 atgcatcggt gaagcgttgc gagcggtcat acgcttcctt ttgcactttg atgtaagtcg
     2641 tcacattctc gaccgaagga tgatccaacg cgcgttcctt cttggccgac accagttcac
     2701 gcaataccgt catcggaagg gcgttcaact tcacgccctc gaccgaaagc cccttaaaca
     2761 gagttcggta cttcgacggg tccagccaag cctctggtgg taagctatcc atcgtaacgg
     2821 ggacaggact ctcttctgcg tcctcctctg cctcggctga cgggtcctga taagagaacc
     2881 acccctgcct gggatggtcg ataaaagtct gagacccagg aggctcagcg gccagcacag
     2941 cactgctcac cactcccacc gagcagaagg tcacgcaata ccaagctaga gcatgcagca
     3001 gttttgtcgt ttccatagca gccacacaaa atcctctcca acggctggca cgattttccc
     3061 gagcccccac atcatgagtg tccgtcctag aggctgacag cacgggggag ggagtcctgt
     3121 gtttggaatc ggccagagta attgaaactt gtactggctt ttcacaatga gtgcggtcgg
     3181 gatgtcctca cacagcagcg gaccattagc ggaggtccaa aactctgttc ccgcgcgggc
     3241 cagtttcgct aacaatcgcg tcatcgacag cgcggcaggt ttgaggtccc catccgctcc
     3301 tgagacatgc ccagtcggcg gatacatgaa tccaaagctc ccggcgcacc agaacagcgg
     3361 atcaattggg aaaccgactg ctgcagctac cgaatcggcc gcgcaggccg cgagtgccac
     3421 cggattggcc aggaccaatg cctccggaaa gagaatgagt gaaatcaggt cgtcgtccca
     3481 caagggatca atctcggagg cataggccaa atcgatttcg tctgcgatcc cggtaggatt
     3541 cagacacaga atgtccattc cgataccaac gatggcccat aggggaaaaa cgaaataatg
     3601 cacatgaaaa aaggcataat ttagctcgga gtccatctgt gtcgtagcca tgccacgccc
     3661 gatgtaactg gtccccccgg ccaaagacac accgagcgtc ggaaagcaca gtggatcgtg
     3721 gaccgcctcg acaagattct tcggctccca gatgcccaga ggtagcccag gaatgcagat
     3781 cggaccaaga cacacacacc cacacaactg cggcggaaac ggggcgtgtc caggaccgat
     3841 ggccggttcg gtaaaatctt ctcccaggtt aaacaccgtg accccgctga gagaaatggg
     3901 aaacatgcac tcccaacacg tcgtagcgag actattcgcc aacacattgg tccctgtcgt
     3961 gcacaccgcc tctgcagagc ccgacggcca cagagtcacc acgccgatca ggacacaact
     4021 caggcagacc agcagcagca tatgctgtat ccgtcttttc gagagcttat tcatgaatcg
     4081 ccacctcctc cacccgcaga tgccgaccta gctgagagac gatactgggt accgcaccga
     4141 cccccatgcg actgacaagc agaggattcg cccaatagac ccgctgatga agttgctcca
     4201 tcactttctg cacgtcgccg tcagagatga tcagcttcac aaacttcgga tcatgctctt
     4261 tcaaatactg ctgcacccaa cctacctgtg aggaactggt gccatcgaaa aacaccagag
     4321 ttcgaatcca ccgtaccttg tcgaagggat taattttggt gccggacttg attaacaccg
     4381 tgttcgtcgt ggggtcctta atatcctcgg gatactcgac cgttggatca acgagcacga
     4441 ccctcgcttc ttcggcatgc ggaagggcca accctgggcc ctttgcgaac tgacgtttga
     4501 gggcgtcgcc agaccgccgt tgaatatccg gccaatccgc cttcgccacc cgctccttca
     4561 tcacatcgat catgtcccgc tccgcgatgt cggctccacg tgcgtcgccc tgcaccttca
     4621 tactctgccc aatggtcgga acctgtggcg gagcttcttc cgtcggcccc ccctgaagaa
     4681 accctcggtg ccggcgctcg aaccattcga tccatggcgt cacaccggcc tgttccttcg
     4741 acaacaacgt caggagatgc gcgaccgaag ctcctccgct cgctattagg aatttctcgt
     4801 ctttcacaaa caccagggcc ggaacttctc ccgcaccaac cgtccggtaa atgactggat
     4861 caatcccgat tccaaccagc agtgagtcat ctggatgatc cccggtgaag agcagatgct
     4921 tgatccgctc ctgtgtaacc ttgaacgaat cctgcaccag tccacgcaag agcaccttgg
     4981 cgccaagtaa tttcgcctct cgcaggtagt ctttgagggt gtcgttcggt aacgagaagg
     5041 agagtagaag gtagacgccg tctcggcgca cttcttttgg tgactcctga ggacgcccat
     5101 gccgcaacgc ttggcgcgag taccttgcga tgtcctgcgc catctcctca ggagatggga
     5161 tacttggacg tactgctgat tgggagcttc ccgcgaaaac agaagacccg atgctcagaa
     5221 gggagagccc tgcgaccgct accacacaga caataccgct cgatacgttc acggtacctc
     5281 catttcagaa tgtctgtatg gagtattacc gacctagcta gatgagtggt gtcaccgtgc
     5341 cgagaatatc cgccttccga attaggccat agtaccgaga gtcgaacgac cgcgggtgtg
     5401 gcagcgccac atagtattcc ccttccggaa taacctccgg aagtgacgct agatcgatcc
     5461 gttgcccgta acgatcgatc ccaaatccac gccccatgga ttgcccgttg atatagacct
     5521 cgtcctcttt gatcacgata tggtcacccg gcactccaac gattcgtttc atccaccgat
     5581 tccccacgtc cgcatagtca cggtgtaaat ggcctggctg cacgcgttcc acgtagtccc
     5641 gccgcatgat gaacgccacg agatcccctc gtcctacggg ctgccctttt tgaatgagga
     5701 aaaagttatg gtgcggccaa ctcccgctca tgcgagcggc gacctcatac caaggggcga
     5761 taacgccggc agtaataatc gcaccacaga acaacatcaa ccccacatgc gtccagcgat
     5821 gccgatcaag ccagtcgagg attgtcggcg cccgcttgag cacgttcagc agcgataatg
     5881 cattgaatag cttcatcgat ggagagctcc tttggtcctt cttttctcac gcgatcaatc
     5941 atcgacagtt catccggatt cgtcgtaaac aacagctgtg tctcacgcgg cacggttagc
     6001 ctgcaaatgc ctctcccaaa tggggtgatc ataaaaatct cagagtactc cgtggtcttt
     6061 gtgacggatg acaggagtcg gtattccatt tccgacaata caatgcgccc attgttccta
     6121 aacgccgcga tgctttcggt cttttgcttg agcagtccca caaattccgc attctcaag
//
LOCUS       DAOQEW010000207         6120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4875_length_6120_cov_8.929431, whole genome shotgun sequence.
ACCESSION   DAOQEW010000207 DAOQEW010000000
VERSION     DAOQEW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_6120_cov_8.929431"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1086
                     /locus_tag="PLS35_16625"
     CDS             211..1086
                     /locus_tag="PLS35_16625"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNI20347.1"
                     /translation="MMNWGPELAVILGIIEGLTEFLPVSSTGHLILVGHALGFTGDIA
                     SNVEISIQLGSILAIIAYERAKLASLASHALREQQEFRSLIATRGTKSWATTLQESIN
                     AQPNLWFVIGLGLAFLPAALVGFVAHKSIKAYLFSPTTVAISLIVGGVIILVVERMQS
                     RVRVKELLQVTPRSALLVGLAQCASLIPGMSRSGSTIVGGLLAGLDRRVATEYSFFLA
                     LPTMIIATIYQMLKSQASFSQDDYVALGIGLVVSFVVAWAVIAAFLTFVQRHSLSVFA
                     YYRMVLGVIVLLVVR"
     gene            1115..1381
                     /locus_tag="PLS35_16630"
     CDS             1115..1381
                     /locus_tag="PLS35_16630"
                     /inference="COORDINATES: protein motif:HMM:NF021169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HNI20348.1"
                     /translation="MASNMDSVHVYDTWVKGKNGKLHFDVMTTSQEKALALAKQYLVG
                     IGEPQAVITLKECQFCHSEPLAMFSVEQQRQFREQGGFIITLPV"
     gene            complement(1400..1921)
                     /gene="lspA"
                     /locus_tag="PLS35_16635"
     CDS             complement(1400..1921)
                     /gene="lspA"
                     /locus_tag="PLS35_16635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNI20349.1"
                     /translation="MSQSIRYLLLASLSLAIVVLDQVTKVYVMETMRLHESIPVIANL
                     FSITYIRNPGAAFGFLSSSSSSFRFVFFGLTSIFAVGLLGMIMVRMPKEDWMGRLSVA
                     GILGGAIGNLLDRLRYGEVIDFLDFYVNGYHWPAFNVADSAITVGVVFLILHFATEKE
                     PADQPVASEQPPS"
     gene            complement(1918..4737)
                     /gene="ileS"
                     /locus_tag="PLS35_16640"
     CDS             complement(1918..4737)
                     /gene="ileS"
                     /locus_tag="PLS35_16640"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNI20350.1"
                     /translation="MDYKSTLNLPKTDFPMKANLPQREPEMLARWAQERLYERIQESR
                     QGREQYILHDGPPYANGRIHIGHALNKILKDIIVKSKTMSGYQVPYVPGWDCHGLPIE
                     HQVLKELGDKKRDLDALAIRKLCQEYAAKYVAIQREEFQRLGVLGEWQQPYLTLNPAY
                     EGTIIREFGRFVERGGVYKGLKPVLWCTQDQTALAEAEVEYDDHTSPSVYVKFPLVSP
                     PSVLSKRFGPVSFPVEATRASVLIWTTTPWTLPANQAVCLHADIDYAFVLVGDELLVM
                     AEKLVESVAKACNIPTYTIVAVKKGREGFEGLEAQRPLTTGLSPLLLGDFVTLEQGTG
                     CVHIAPGHGMEDYLLVLDHNAKASVGERLEILAPVDNGGKFTEAVPEFAGQHVFKANP
                     KIVERLKGNGRLLGHGSLNHSYPHCWRCKQPVIFRATEQWFVSMETNDLRKEALAEIE
                     RVRWIPAYGRDRINGMIENRPDWCLSRQRVWGTPIPGFTCLTCGKVLANPRVIDHVAD
                     LIGQHGTDYWFANQAGALLPAGTQCEGCGGTDFEKERDILDVWFESGVSYAAVLKPRQ
                     WYPADLYLEGSDQHRGWFHSALLSGVITDHRAPYKAVLTHGFVLDGAGRKMSKSAGNV
                     VAPQDVIKQSGAEILRLWVSAQDYREDLRISQEILNHLIEAYRKIRNTCRFLLSNLYD
                     FDPAQHRVPFEQLPELDRWALMRLSDLIPRVRKGYDEFEFHTIFHALNNFCSVDLSAV
                     YLDILKDRLYTFRKDSPLRRGSQTVLFDIVVALTKLMAPVLSFTAEEIWRTLPESVRT
                     EAKADSVHIAMFPEVDPRWADPMLAQRWEQVLEVRSAVQAALEVKRREKVIGAPLEAR
                     VVIEANSERYEFLKRYEQELSSFFIVSDVVLTQAGHLAESPGYSVTVDKATGAKCERC
                     WNYRSAVGTSADHPTLCDRCIEAVR"
     gene            complement(5000..>6120)
                     /gene="nadA"
                     /locus_tag="PLS35_16645"
     CDS             complement(5000..>6120)
                     /gene="nadA"
                     /locus_tag="PLS35_16645"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNI20351.1"
                     /translation="TERLQVKTVTTLPRPITDYQSLSAEELFERTRAAKRALGDRVMV
                     LGHNYQRDEVIQHADFRGDSLILAQVAEQRSDRPYVVFCGVHFMAETADVLSRSNQTV
                     ILPDMAAGCSMADMAAIEQVEQCWDALGRVVPVEETVMPAVYVNSAAILKAFCGEHGG
                     LTCTSSNARKVIEWSWARREKILFFPDEHLGRNTANKMGIPREQMIVWDPYMPRGGNS
                     VEAIQRAKLILWKGHCSVHQMFQPSHIDYVRKKHPEVKVIVHPECHEDVVNKADLVGS
                     TEYIIRTITAAPAGTTWAVGTELNLVNRLKHEQADKKIFFLSSTVCQCATMYRIDAPH
                     LCWALENLADGHVVNRIVVPDDEKQLAKVALDRMMALS"
BASE COUNT         1299 a         1855 c         1715 g         1251 t
ORIGIN      
        1 tctcttgttg tgtgtatgcc atcacttcaa ctttgtcaac aagtttgccg attgcgcagc
       61 accggttgcg atgacgcgcc acatcgagaa agaggtcgcc gtaaactcga cagcgcttga
      121 ctgccggtga ccagtctggg attatgcaac gacgattcgt gacaactacg caaccaaccg
      181 cagcatccca cacaccatct cacacacact atgatgaact ggggcccgga gcttgccgtc
      241 attctcggca tcattgaagg actgacggaa tttctccccg tatcctcgac cggccatctc
      301 atcctcgtcg ggcatgccct cgggtttacg ggggatatcg cctccaacgt ggagatctcc
      361 attcagctcg gctcgattct cgcgatcatc gcttacgagc gcgccaagct ggcgtcgctc
      421 gcgtcccatg ccctacgcga acaacaggag tttcgctcgt tgatcgccac ccggggcacg
      481 aaatcctggg caacgaccct tcaggaatcg atcaatgccc agcccaatct ctggttcgtg
      541 attggattgg gcctggcctt cctgcccgcc gcgctggtgg gattcgtcgc gcacaagtcg
      601 atcaaggcct atctcttctc gccgacgacc gtggcaattt ccttaatcgt cggaggcgtg
      661 atcattctgg tggtcgagcg aatgcagagc cgcgtgcgcg tgaaagagct gctacaggtg
      721 acgccacgat cggccctgct ggtcggcttg gcccaatgcg catccctgat ccctggcatg
      781 tcccgctcgg gttccacgat tgtcggcggt ctgctggccg gcctcgaccg gcgcgtggcc
      841 accgaatatt cgttcttcct ggccctcccg accatgatca tcgccacgat ctaccagatg
      901 ctcaaatcgc aggcctcgtt ttctcaggat gactacgtgg cgctcggcat cggcctggtg
      961 gtgtcgtttg tggtcgcctg ggccgtgatc gccgcctttc tcacctttgt gcaacgccac
     1021 agcctcagcg tgttcgccta ttaccgcatg gtactgggcg tcattgtgct gctcgtggtc
     1081 cgataaccaa accataaccg ggagctgaat cgtcatggca agtaacatgg acagcgtcca
     1141 cgtgtacgat acgtgggtca aaggaaagaa cggcaaactg cacttcgatg ttatgacgac
     1201 cagtcaggaa aaagccctgg cgctggcgaa gcaatacctg gtgggaatcg gcgagcctca
     1261 ggccgtgatt acgctcaagg aatgccagtt ctgccacagc gaaccgctcg cgatgttttc
     1321 ggtcgagcag caacggcagt ttcgggagca gggcggattc atcatcacct tgccggtgtg
     1381 atggtcggaa atgcgctggt caggaaggcg gctgctcgga agcgacaggc tgatccgccg
     1441 gttccttctc cgtcgcgaag tgaagtatta gaaacacgac tccgacggta atcgcggaat
     1501 cagccacgtt aaacgccggc cagtgatacc cgttgacgta aaaatcgagg aagtcgatga
     1561 cttctccgta acggagccga tccaataagt ttccgatggc tccgccgaga attcccgcca
     1621 cgctgagccg ccccatccag tcttctttcg gcatacgcac catgatcatg ccgagcaagc
     1681 ccaccgcaaa gatcgatgtc aggccgaaga agacaaacct gaacgagctg ctactcgatg
     1741 agaggaaacc gaaggccgca ccgggattgc gaatgtaggt gatactgaac agattggcga
     1801 tgactggaat ggactcatgg agccgcatgg tctccatgac atagaccttc gtgacttgat
     1861 ccaacacgac aatcgccagg ctcagcgagg cgagcagcag ataacggatg gactggctca
     1921 acggacggcc tcaatacagc gatcgcacag tgtgggatgg tcggcagagg tgcccacagc
     1981 ggatcgatag ttccagcagc gctcgcactt ggcgcccgta gccttatcca ccgtcacgct
     2041 atacccgggg gactccgcga gatgaccggc ctgagtgaga accacatctg acacgataaa
     2101 gaaggacgag agctcctgtt cgtaccgctt caggaattca taccgctccg aattcgcctc
     2161 gatcacgact ctcgcttcca gcggcgcgcc gatcaccttc tcgcgacgct tcacttccag
     2221 agcggcctga acggcggacc tcacctcgag cacctgctcc caccgttgcg ccagcatcgg
     2281 atcagcccag cgcggatcga cctctgggaa catggcgata tgcacactgt cggcttttgc
     2341 ttctgtgcgc acagactcag gcaacgttct ccagatttct tcggcggtga aactcaacac
     2401 gggcgccatc aatttggtca acgcaaccac gatgtcgaac aacaccgtct gcgaaccacg
     2461 ccggagcgga gaatccttcc ggaacgtata caaacggtcc ttcaggatat caaggtacac
     2521 ggcgctcaga tcgacagaac aaaaattgtt caacgcgtga aagatcgtgt ggaactcaaa
     2581 ttcgtcatac cccttgcgca cgcgcggaat caggtcgctc agccgcatga gggcccagcg
     2641 gtctaattcg ggcaactgct cgaagggcac gcgatgctgc gccggatcga agtcgtacag
     2701 attgctcagc aggaaccggc aggtattgcg aatctttcgg tacgcttcga tcaggtgatt
     2761 caggatctcc tgtgaaattc gaagatcttc gcgataatcc tgggccgata cccagaggcg
     2821 caggatttcc gccccggatt gtttgatgac gtcctgagga gccacgacgt ttcccgccga
     2881 cttcgacatc tttcgccccg ccccgtcgag cacgaaacca tgggtcaaca cagctttata
     2941 cggcgcacga tgatcggtga tcacacccga caacagggcg ctgtggaacc aaccgcgatg
     3001 ctggtccgac ccttccagat acagatccgc cggataccat tgtcgaggct tgagcaccgc
     3061 cgcataactc acgccggact cgaaccagac gtccagaatg tcgcgctcct tctcgaaatc
     3121 agttccgcca caaccttcgc actgcgtgcc ggcaggcagc aacgcgccag cctggttggc
     3181 aaaccagtag tcggtgccat gttgcccgat cagatccgcc acatgatcga tcacgcgggg
     3241 attggccagc accttgccgc acgtgaggca ggtgaagccg gggatcggcg tgccccaaac
     3301 ccgttggcgc gacaggcacc agtcagggcg gttttcgatc attccattaa tgcggtcgcg
     3361 gccataggcc ggaatccagc gcacccgttc aatctctgcc aacgcttctt tccgcagatc
     3421 attcgtctcc atagagacga accattgttc ggtcgcgcga aagatcaccg gctgcttaca
     3481 tcgccagcaa tggggatacg agtggttgag cgagccgtgt ccgagcaagc ggccattgcc
     3541 cttcaggcgc tccacgattt tcggattggc tttgaagaca tgctgaccgg caaactccgg
     3601 aacggcttcg gtaaacttcc cgccattgtc cacgggagcc aggatttcga gtcgctcgcc
     3661 gacactcgcc ttggcattgt gatccagcac cagcagatag tcttccatgc cgtggcccgg
     3721 cgcgatgtgc acgcatccgg ttccctgctc caatgtcacg aaatcgccca ggagcaatgg
     3781 agagagacct gtggtcaacg ggcgttgcgc ctccaagccc tcaaatccct cgcgaccctt
     3841 tttcacggcg acgatcgtgt acgtggggat gttgcacgcc ttggcaaccg actcgacaag
     3901 cttctcagcc atgaccagca attcgtcccc gaccagcacg aaggcatagt cgatgtcggc
     3961 atgaagacaa acggcctgat tggccggcag ggtccacggc gtcgtcgtcc agatcagcac
     4021 cgacgcgcgt gttgcttcga cagggaacga aaccggccca aacctcttgc tcaacacaga
     4081 aggcgggctc accaggggaa actttacata gaccgagggc gaggtgtggt cgtcgtattc
     4141 aacctcggct tcggccagtg cggtctggtc ttgcgtgcac cacaagaccg gcttcaagcc
     4201 tttatagacg ccgccgcgct caacaaaccg gccgaactcg cggatgatgg tgccttcgta
     4261 ggccggattc aacgtgagat agggttgctg ccattcgccg agcacgccca gccgctggaa
     4321 ttcctcccgc tggatggcca catacttggc cgcatactcc tgacacagtt tgcggatcgc
     4381 gagcgcatcc aggtcgcgct tcttgtcgcc cagttccttc aggacctggt gctcgatggg
     4441 tagaccgtgg caatcccagc ccggcacgta cggcacctga taaccggaca tcgtcttcga
     4501 tttgacgatg atgtctttca gaatcttgtt cagggcgtgg ccaatatgga tccgcccgtt
     4561 cgcatagggc ggcccgtcat gcaggatgta ttgttcacgc ccctggcgag actcctggat
     4621 ccgttcgtac agccgttcct gtgcccaacg ggccagcatc tccggctccc gttgcggcag
     4681 gttcgccttc ataggaaagt cggtcttcgg taaattgagg gtcgatttat agtccataga
     4741 ttgatctagc tgcgagacga aatggccatg aagtacgcgt aaagagagga ctataccgaa
     4801 tcggggcgag aggaaccagt ggtttgttgg cagccggaac cattagcaag gtgactggta
     4861 ggacctggct gtcctgcaag ctgttcagaa aggtcgaccg gcaaggccgc agcgagggag
     4921 catgtgaggc gtacccttgc ggtacgttga acatgcgagc gacgcgagaa cgcagccagc
     4981 ggcctttctc aacagtttgc tagctgagcg ccatcatgcg gtccaatgcc accttcgcca
     5041 gttgcttttc gtcgtccgga acgacgatcc ggttgacgac gtgtccatcg gcaagattct
     5101 ccagcgccca acagagatgt ggagcgtcga tgcgatacat cgtggcgcat tggcacaccg
     5161 tggaagagag gaaaaagatc ttcttatcgg cttgctcatg cttcaggcga ttgacgaggt
     5221 tgagttctgt cccgaccgcc caggtggtcc ccgccggggc cgccgtgatc gtgcgaatga
     5281 tgtactcagt cgatcccacg agatcagcct tgttgacgac atcttcgtga cattcgggat
     5341 gcacaatgac ttttacctcg ggatgcttct tgcggacgta atcgatgtgg gacggctgaa
     5401 acatctgatg cacgctgcaa tggcctttcc ataagatcag cttcgcgcgc tgaatcgcct
     5461 ccaccgagtt gccgccacgc ggcatatacg gatcccagac gatcatctgc tcgcgcggga
     5521 tgcccatttt gttcgccgta ttccggccca gatgctcgtc ggggaagaac aggattttct
     5581 cacgtcgcgc ccagctccat tcgatcacct tgcgcgcgtt tgacgaagtg caggtcagcc
     5641 cgccatgctc gccgcagaaa gcctttaaga tcgccgcgga gttcacgtag accgccggca
     5701 tgaccgtctc ctccaccggc accacccgac cgagcgcatc ccagcactgt tcgacctgct
     5761 cgatcgccgc catatcggcc atagaacagc cggccgccat gtcagggaga ataacagtct
     5821 ggttggaacg gctcagcaca tcggctgttt ccgccatgaa gtgcacgccg cagaacacca
     5881 catagggccg atcggaacgc tgctcggcga cttgcgcgag aatcagcgag tcgccacgga
     5941 agtcggcatg ttggatgact tcatcgcgct gataattatg gccgagaacc atgacccggt
     6001 caccgagcgc ccgtttggcc gcacgggtcc gttcgaagag ttcttcggca gacagagact
     6061 gatagtcggt aatgggacgg ggaagcgtgg tgaccgtttt cacctgcagc ctttcggttg
//
LOCUS       DAOQEW010000208         6116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4880_length_6116_cov_7.907111, whole genome shotgun sequence.
ACCESSION   DAOQEW010000208 DAOQEW010000000
VERSION     DAOQEW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_6116_cov_7.907111"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLS35_16650"
     CDS             complement(<1..787)
                     /locus_tag="PLS35_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNI20352.1"
                     /translation="MVYRHERRRVRSVLAVTAMVAATFLSGGLPPRAGQAEAAGVPPA
                     FAQGFSEIVKAVTPAVVNIAVTGGGEGRREGRRQLPPGGPFGGPPPGPGGPGDEPPGM
                     EPPGGPPGGGPHRPEQSAGSGVILDPNGYIVTNNHVVEGATQITVTLSDRREFPAKII
                     GTDPKTDLAIIKIEAKDLSSMKWADYDELQVGDVVLAVGSPFGLSSTVTLGIISALGR
                     GNVGIADYEDFIQTDAAINPGNSGGALVNMQGKLIGINTAIFSR"
     gene            complement(959..1723)
                     /locus_tag="PLS35_16655"
     CDS             complement(959..1723)
                     /locus_tag="PLS35_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20353.1"
                     /translation="MRRSANCLASLVGMVIVTSCAVASPPSRLAQYVGSVVPEEAATA
                     TLPTSRPLHAALVVVADQSAPDSAPVLPDEALARVGALLTGEINRGYPVAVDRVVKLD
                     GMSPGGTAPSWPEVARQQGVQYLLVTVLSSTEQEYPISLFLGWTTHQQPGFRRDNWSL
                     AEAALIDGSTGRTVLQAEGRAMATLDRPTAPGISQWYPVIYLRPQDPERRIWPPTYEG
                     APLTLRLVSMEQAAKRLAGNLQRAWVEQRDLELAAR"
     gene            1910..2725
                     /locus_tag="PLS35_16660"
     CDS             1910..2725
                     /locus_tag="PLS35_16660"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI20354.1"
                     /translation="MTLCSTQTPSLSVIIPAYNEARRLPLFLQRVIAHLDQRGQFYEI
                     LVVDDGSQDNTAHAVRMAAHHCPHLRLIQLTCNMGKGAAVRRGMQAARGTIQLFADAD
                     GATPIEELGRLEAALAAGADLAIGSRALASHDPAFTVHARWHRSLLGGLFNHVVQRLG
                     VPHIADTQCGFKLFRQSVAQDLFAVSCVDGYAFDLELLHIARQRHYRIAEVPINWTDQ
                     PGSKVRPWRDGSLMLRELLAIRKREAQGLYQPQSRPASSAVEPDLVTVEPTHS"
     gene            complement(2742..4277)
                     /locus_tag="PLS35_16665"
     CDS             complement(2742..4277)
                     /locus_tag="PLS35_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20355.1"
                     /translation="MKAEPVRGSFPLPALMLILDVLLGAFVLNLVLQPLVEPDLGWHL
                     RAGLDLIAQGWRLPDTDPYSHTMPDWHWVEHAWLTDGLLGLIYRMLEPSGGLGLIAFF
                     GGVTALAWWIAAGVASVHRTCRLAAMVASLWVALPFLGARTQLISLLGVAVVLTIWNR
                     MQRGQPQWVWALPPLFLLWANLHGGFTAGLFLITVLLSLSFLVKICVEWKVVQAPRDE
                     PVLSWDVIRLCAFAILVSFAVTCLNPYGWRLYVEIYESLTDRFMIDTLREWQPVSFHG
                     WAGRAYGFYLLGFIGLMAGWYRRVEPVRWVLLALMLGLSLLHWRNVTLFLIVSLPLAA
                     ELGAAAADSFLRRVPVLQAHAAGTVLVVSIMAATALYALGTEHLEHVWQSGRTPDRYF
                     ERTEYPIEAVRWIRAHRDETGTRLYNDYSYGGFLLWQLPGERIFIDGRMPAWRIKDRW
                     IFHDYVELNREDSPQLALLDRYGVDWAMIPRGSALAVALEAHPAWKNVYADAKVVIVR
                     RQS"
     gene            complement(4267..6000)
                     /locus_tag="PLS35_16670"
     CDS             complement(4267..6000)
                     /locus_tag="PLS35_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNI20356.1"
                     /translation="MVVLGLSNMRDAAAALVADGRIVAAAEEERFVRQKHVTALPVHA
                     MQYCLREAGMALRDVDAIAVPWKYWQVGRRLRLAVTAMLRSTQLFRVKGTRSLERASQ
                     EWKELFRLQQELSTRVEPGAGRPVFLDHHLCHAASSFLVSPFERAAILVVDGASESDT
                     TLLATGEGNQISVLERTPLPHSLGQFYAAMTAFLGFRPDQDEYIVMGLASSGEPSFAP
                     TLSREILRVLPDGRFELNTRLLDFHLARAGHFVREFIRLFGPPRRPQDEITQRHRDLA
                     ASAQFVLEETLLHLGRRLRSLTQATSLCLAGGVAYNCVANGRLRAELGFDQVYVPPAA
                     GDSGAALGAALWWTARRGAATSKTVMPDAYLGPQYDESACRAALSKTGLVAEHLADDR
                     LYERVASELAHGRLVFWYQGRMEWGPRALGNRSLLADPRREDMRELINSKVKCREAFR
                     PFAPSVLAEHAEEFFHVPAPSAFMQFTVRVKASAKGLLPAVTHVDGTARVQTVTREAN
                     PRFYDLLATFGRLTGVPVLLNTSFNVQEPIVCSPDEAVRCFQRTQVDWLVLGNLLVGR
                     PSPMPPQHHEG"
BASE COUNT         1342 a         2004 c         1704 g         1066 t
ORIGIN      
        1 tgcgggagaa gatggccgta ttgatgccga tcaacttgcc ttgcatgttg accagcgcac
       61 cgcccgaatt gccggggttg atggccgcat cggtttgaat aaagtcttcg tagtcggcga
      121 tgcccacgtt cccccgaccc aacgcgctga taatgccgag ggtgacggtt gaactgagcc
      181 cgaacggact acccacggcc agaaccacat cgccaacctg cagctcatcg taatccgccc
      241 acttcatcga gctgagatct ttggcttcga tcttgataat cgccagatcg gtcttgggat
      301 cggttccgat aattttggcg gggaattcgc ggcgatccga gagggtcacc gtgatttggg
      361 tggcgccttc gaccacgtgg ttattggtca cgatataccc gttcggatcg aggatcacgc
      421 cggaccctgc gctttgctcg ggacgatggg gaccaccgcc cggagggcca ccgggaggct
      481 ccatgccagg aggttcatca ccaggaccac cgggaccagg aggcggaccg ccgaagggac
      541 cgcctggggg aagctgacga cgaccttccc ggcgtccctc gccaccaccg gtcaccgcaa
      601 tgttcaccac cgcaggggtg acagctttta cgatttcaga aaacccttga gcgaatgccg
      661 ggggaacacc agcagcctcg gcctgaccgg cccggggcgg taatccaccg gataagaatg
      721 tggcagcaac catcgcggtt actgcgagta cggaccgcac tctcctgcgc tcgtgtcgat
      781 acaccatgtg tactcctctg gaaacctgag attgagggac catcctccgc gccaacgttg
      841 aacgtttcat tctacactaa tcacagaatc gccttcaaag aggaaaacgc ggctccgaac
      901 ctgatgccat catcgtctgg accgagcctg cgtcacctcc gaccgacggc tcagagcctt
      961 agcgcgcagc gagttcaaga tcccgctgtt ctacccaggc tcgctgcaga ttgccggcca
     1021 accgcttcgc cgcctgttcc atcgagacca gacggagtgt gagcggagcc ccttcatagg
     1081 tgggcggcca aatgcgtcgc tccgggtctt gcggccgcag ataaatgacg ggataccatt
     1141 gcgaaattcc cggcgccgtc gggcggtcca acgtggccat ggctcgtcct tccgcctgca
     1201 gcaccgtcct cccggtcgat ccgtcgatca acgccgcttc ggcgagagac cagttatcgc
     1261 gccgaaatcc aggctgttgg tgggtcgtcc accccaaaaa gagagaaatc ggatattcct
     1321 gctcggtgct cgacagaacc gtcaccagca gatactgaac cccttgctgc ctggcgacct
     1381 caggccagct cggcgctgtt cccccgggtg acatcccatc aagcttgacg actcggtcaa
     1441 cagcgacggg atacccacgg ttgatctccc ccgtgaggag cgctcccacc cgcgccaggg
     1501 cttcgtctgg caaaacgggc gctgaatccg gagccgactg atccgcgacc acgaccagcg
     1561 cggcatggag cggacgcgaa gtgggaagcg tcgccgtcgc cgcctcttcc ggcacgacgg
     1621 atccgacata ctgagcgagg cgactgggcg gactggcgac ggcacaactc gtcacgatca
     1681 ccatgccgac gagcgacgcc aagcagttcg cgctgcgcct catgacatcc tccggaccgg
     1741 atcgacgcca aggataccgc cgtatctgag atacgatcac tcccgcgctc tgcctctgtc
     1801 aagatgtgcc aaccatggct tgcatttcga tcatggctca gctaccgtgg tgcacccttc
     1861 gtcgccggac gaagcggtcg cgtcacactc ccaccggggg cgttccaacg tgactctgtg
     1921 ctcgacccag acaccatccc tttcagtcat cattcccgcc tacaatgagg cgcgccggct
     1981 cccgctgttt ttgcaacgtg tgattgccca tctggaccag cgcgggcaat tctatgaaat
     2041 tctggtggtg gacgacggga gtcaagacaa caccgctcac gccgtcagga tggcggcaca
     2101 ccactgtcct catctgcggc tgattcaatt gacctgcaac atgggcaaag gcgcggcggt
     2161 tcgacggggc atgcaggctg cgcgcggaac catccagcta tttgccgacg cggatggagc
     2221 cactcccatt gaagaactcg gacgattgga agcggcgctc gcggccgggg ctgaccttgc
     2281 gatcggatca cgggccctgg cctcccacga cccggccttt acggttcatg cccgatggca
     2341 ccggagcctg ctggggggcc tcttcaacca tgtggtgcaa cggctgggcg tgccgcatat
     2401 tgccgacacg caatgcgggt ttaagttgtt ccgacaatcg gtggctcagg atttgtttgc
     2461 cgtatcctgt gtcgacggct acgcattcga cctggaatta ctgcacatcg cccgccaacg
     2521 gcactaccgc atcgcggaag tccctatcaa ctggaccgat cagccgggct cgaaagttcg
     2581 cccttggcgc gacggctcct tgatgctgcg ggaacttctg gccatccgta agcgcgaggc
     2641 ccaaggcctt taccagcccc aatcacgccc ggcatccagc gccgtcgaac cggacctcgt
     2701 tacagtcgaa ccgacgcatt cctaaacgac gcaccctgcg gtcacgactg ccggcgcaca
     2761 atcaccacct tggcatcggc ataaacattc ttccaggcag gatgcgcctc cagagccact
     2821 gccaacgcgc tgccacgcgg gatcatcgcc caatccaccc cgtatcgatc cagcaaggcc
     2881 aattgcggcg aatcttcgcg attgagttca acgtaatcgt gaaagatcca tctgtccttg
     2941 atccgccagg caggcatgcg tccgtcgatg aagattcgct cgcctggaag ttgccatagc
     3001 agaaacccgc cgtagctata gtcgttatag agcctcgtcc ccgtttcatc ccggtgagcc
     3061 cgaatccacc gcaccgcttc gatgggatac tccgtgcgct caaaataccg atcgggagta
     3121 cggcctgatt gccacacatg ctccaggtgc tcagttccca aggcatacag cgccgtcgcc
     3181 gccataatgg ataccacaag gacagtgccg gcagcgtggg cttgcagcac aggaacgcga
     3241 cgcaggaagg aatctgccgc agccgcacct aactctgccg ccaacgggag actcacgatc
     3301 aaaaacagag tgacattccg ccaatgcagc aaagacaatc ccagcatcag cgccagcagg
     3361 acccaccgca ccggctccac tcgccgatac catccggcca tcaacccgat aaatccaagg
     3421 agatagaacc cataggcccg tccggcccac ccgtgaaacg acaccggctg ccactcccgc
     3481 agggtgtcga tcatgaagcg atccgtcaat gattcgtaaa tctccacata gagacgccag
     3541 ccatagggat tcaggcaggt cacagcaaaa gagacaagaa tggcgaaagc acacagccgg
     3601 ataacatccc agctgagcac cggttcatca cgcggtgcct gaacgacctt ccactcgacg
     3661 cagattttca cgaggaatga gagcgacaac aacacggtga ttaaaaacag acccgccgtg
     3721 aagccgccgt gaagattggc ccacaagaga aacagaggcg gcagagccca gacccattgc
     3781 ggctgtcccc gctgcatgcg attccatatc gtgagcacaa ctgccacgcc aaggaggctg
     3841 atcaactgcg tgcgcgcgcc gagaaagggt aaggccaccc agaggctcgc gaccatcgcc
     3901 gctaaccggc acgtgcgatg gacggaagct actcctgccg cgatccacca ggccagcgct
     3961 gtgactcctc caaaaaacgc aatcagcccc aatccgccag atggttcaag catccgatag
     4021 attaatccca acagcccatc ggtcaaccag gcatgctcca cccaatgcca atccggcatc
     4081 gtatgggaat aggggtcggt atccggcaaa cgccaccctt gcgcgatcaa atccagtccg
     4141 gcccggagat gccatcctag atccggttcc actaagggtt ggagcaccag attcaagaca
     4201 aacgcgccca gcaacacatc cagtatgagc atgagggcag gaagaggaaa ggaaccgcga
     4261 accggctcag ccttcatgat gctgcggcgg catcggtgac ggccgtccca ccagaagatt
     4321 gccgagaacc aaccagtcca cctgggtacg ctgaaaacaa cgcactgctt cgtcgggact
     4381 gcacacaatc ggttcctgca cattgaacga cgtgttcagc agcacgggca cacccgtcaa
     4441 tctgccaaag gtcgccagca gatcatagaa gcggggattg gcttcacgcg tcacggtttg
     4501 cacacgagcg gtaccatcga catgcgtcac ggccggcagg agccccttcg ctgatgcttt
     4561 cactcgaacc gtaaactgca tgaaggccga gggggcaggc acatggaaaa actcctccgc
     4621 gtgttcggca aggacggagg gtgcgaatgg tcgaaaagcc tcgcgacatt tcaccttgct
     4681 attgatcaat tcacgcatgt cttcccgtcg aggatccgcc agcaaactgc gatttcctaa
     4741 tgcgcgcggg ccccactcca tccgcccttg ataccagaac accaatcgcc catgggccaa
     4801 ctccgaggcc acccgttcgt acaatcggtc atctgccaga tgttcggcaa ccagtcctgt
     4861 cttcgacaag gcggcacggc aggcggattc gtcatactgc ggaccgagat aggcatccgg
     4921 cataacggtc ttcgacgtcg ccgctcctcg ccgcgccgtc caccagaggg ccgcgccgag
     4981 cgccgccccg gaatctccgg cagccggcgg cacgtacacc tggtcaaacc cgagttcggc
     5041 ccgcaggcga ccgttggcga cacagttgta ggctacccca cctgcgaggc aaagtgatgt
     5101 cgcctgagtg agcgaacgca gacgacgccc cagatgcaac agggtttctt ccaacacgaa
     5161 ttgcgcgctg gccgcaagat cgcggtggcg ctgcgtgatc tcgtcttgcg gccgccgcgg
     5221 aggaccgaac agccggatga attcccgcac gaaatggccc gccctggcga ggtggaaatc
     5281 cagcaggcgg gtattcagtt cgaatcgtcc atcggggagc actcgcaaaa tctcgcgact
     5341 cagcgtgggg gcgaacgacg gctcgccgga cgaagcgaga cccatcacga tgtattcatc
     5401 ctgatccggg cgaaacccca ggaacgccgt catcgccgca tagaattgcc ccagcgagtg
     5461 aggcaacggc gtgcgctcca gtacgctgat ctgattgcct tcaccggtag ccagcaaggt
     5521 cgtatccgat tctgatgctc cgtcgaccac cagaatcgcc gcccgttcaa atggcgagac
     5581 gagaaatgag ctggctgcat ggcagagatg gtggtcgaga aacaccggcc ggccggcacc
     5641 cggttcaacc cgcgtggata gttcctgctg aagccggaag agttccttcc attcttgact
     5701 cgcccgctca agggaccgtg tccccttcac acggaacagt tgcgtcgagc gcagcatcgc
     5761 cgtcaccgca agcctgagac gccgccccac ctgccaatac ttccagggca ccgcgatggc
     5821 gtcgacgtcg cgcaaagcca ttccggcttc gcgcaggcaa tactgcatcg catgcactgg
     5881 cagggccgtc acgtgctttt ggcggacgaa gcgttcttcc tctgcggcag ccacgatccg
     5941 cccgtcagca acgagggcgg ccgcggcatc tcgcatattg gacaaaccta acaccaccac
     6001 gatacgtcaa cgtccttttg tctttttcat tgcctgactg aggagcaagg agcaacgcga
     6061 ctcccgcgcc gatcaatgcg cccgccatga atgccgacca gttggaagaa tcatcc
//
LOCUS       DAOQEW010000209         6113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4885_length_6113_cov_5.687190, whole genome shotgun sequence.
ACCESSION   DAOQEW010000209 DAOQEW010000000
VERSION     DAOQEW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4885_length_6113_cov_5.687190"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLS35_16675"
     CDS             <1..952
                     /locus_tag="PLS35_16675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNI20357.1"
                     /translation="LDKTGTITEGKPTVTDVIPATDGGWSARDLLRVVASLEASSEHP
                     VAEAIVREAGEQLVSLADVDAFQSFAGRGASGVVDGAAVVVGNQALMEEYSVPLADLV
                     ARAEQLAGEGKTPVFIAINGAPAGLIAVADRVKATSQAAIRELKQLGLTVVMLTGDHE
                     RTAQAVAREVEVDRVVAGVLPEGKVTEIKRLRDDGQVVAMVGDGINDAPALAQADIGF
                     AIGSGTDIAIEASDVTLMRSDLRGVASAIRLARTTMRVMKQNLFWAFFYNVLGIPIAA
                     GALYPFTGLMLSPVLASAAMAFSSISVVMNSLRLRTARIN"
     gene            1057..1413
                     /locus_tag="PLS35_16680"
     CDS             1057..1413
                     /locus_tag="PLS35_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20358.1"
                     /translation="MHSATARRLLTIAVIVLFLGFSMNAFACLVPLYGVPNMEAGCPS
                     GNEQPPRTFCDAFKAGVPAHSSYIYPFAMHLSVPLVALDSLGLSCSTEVLTRDLDSSA
                     DSRPQNIFFKTTILRI"
     gene            1825..2334
                     /locus_tag="PLS35_16685"
     CDS             1825..2334
                     /locus_tag="PLS35_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HNI20359.1"
                     /translation="MNTATHATHHHHHGQTHEGKETPTPPLNRLAISATLHCLTGCAI
                     GEVLGLMIAMALGWGTAASIVLAILLAFVFGYGFTLVPLVQGGMTWAKAASVAFASDT
                     VSIAVMELMDNAIMLLIPGAMAAGLTDGLFWGSLVLSFAVAFVVTVPVNRWLIARGRG
                     HAVVHAHHH"
     gene            2388..4253
                     /locus_tag="PLS35_16690"
     CDS             2388..4253
                     /locus_tag="PLS35_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HNI20360.1"
                     /translation="MKRTSITRREILKGAATLGLLAAAHALKPIRLWAGTAAEPPSPE
                     VLTGDLIDLTISEQIFHLNGREAPAVMINGTLPGPLIRLKEGQQATLRVTNRLREVTS
                     IHWHGLLVPPEMDGVPGVSFRGIDPGATFTYRFPIQQSGTYWFHSHSGGQELQGMYAP
                     MIIDPFEPEPFQYERDYVVMLSDWTFESPPTVLSNLKKQAGYYNFQRRTAGEFLADVR
                     RKGWWATVQDYLAWDRMRMDPTDFADVTGYTFTYLMNGRSPESNWTGLFRPRERIRLR
                     VINAAAMTLFDVRIPGLEMTVVQADGQSVQPVVVDEFRIGPAETYDVIVEPTDDGPYT
                     VFAETLDRSGYARSTLATRAGAIAALPDRRPRPLRTMADMGMNHGLASHSSTDAGVHD
                     RHLPTGTMPSHTSPNPGHQQHGRGAGDASAHEARSDEETGDQRPSERPTTEPVPHGPD
                     DHGTGNQMVAEYSSNRMHEPGAGLDASERRVLLYTDLKSVRPFDDHRRPDREIELHLT
                     GHMERYMWSFDGKKYSEAPPIQFRYGERMRLTFVNDTMMEHPLHLHGMWMYLENGAGP
                     YLPRKHTVIVKPAERLSVAISADAPGRWAFHCHLQLHMEAGMFRVVEVLEPTTSS"
     gene            4250..5176
                     /locus_tag="PLS35_16695"
     CDS             4250..5176
                     /locus_tag="PLS35_16695"
                     /inference="COORDINATES: protein motif:HMM:NF017119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HNI20361.1"
                     /translation="MKEPGPVLLLAPLLFLVQLVSSSTMALAEPQEVKLPTVHSSASA
                     HPATPDRMINTHFTYAPEPLDLSLVKATIPPKLSPGRDWPSPVDDAERRTMVLVDVLE
                     YRPQTRSTGSQSDYRWDIEGWYGGDYNRVWFKTEGQRNTAFKADYDVDFQLLYGRFIQ
                     KYYDFQIGGRVETQTFRGANVTRGLAVIGIEGLVPYSYQIEPSLFIDQNGAVSARLAA
                     TKDLLMTQRLILQLRFETNAALQRVEEFTTGTGLNNVEFGVRLRYDIRRGFSPYVGVS
                     LDRSFGETATLIRQEGGSSSQVRFALGLRMWF"
     gene            5510..5881
                     /locus_tag="PLS35_16700"
     CDS             5510..5881
                     /locus_tag="PLS35_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20362.1"
                     /translation="MVHILLIEDTEAVRGLFRAILEQVGYVVREASTGSAGIRLFHES
                     PSDVVITDMYMPDGDGFDVMRTLRHEVPAPKIIVMSGGLGKGEILDAAQLLGADLVLA
                     KPVPMDELLKAVETLIGTSRL"
BASE COUNT         1249 a         1716 c         1868 g         1280 t
ORIGIN      
        1 gttggataaa accggcacca tcaccgaggg caagccgacc gtgaccgatg tcatcccggc
       61 aacggacggt ggatggtccg ctcgtgacct gctcagagtg gtggcgtcgc tcgaagcatc
      121 ctcggaacat ccggtggcgg aggccatcgt gcgggaagcc ggagaacagt tggtctcctt
      181 ggctgacgtg gacgccttcc aatcctttgc gggacgtggg gcgtctgggg tcgtcgacgg
      241 ggcggcagtc gtagttggga accaggccct tatggaagag tactcggttc cgctggcgga
      301 cctcgtcgca cgggcagagc agttggcagg tgaaggcaag acgccggtgt ttatcgcgat
      361 caacggagcc cctgccggcc tgattgccgt ggcggaccgg gtcaaagcga cctcacaggc
      421 cgccattcgc gaactcaagc agcttgggct cacggtcgtc atgctgacgg gagatcatga
      481 gcgaacggcg caggcggtcg cccgcgaggt cgaggtcgac cgtgtggtgg caggcgtctt
      541 gccagagggt aaggtgacgg aaatcaaacg gctccgcgat gacgggcagg ttgtcgcgat
      601 ggtcggagat ggcatcaatg acgcgccggc gctggcacag gctgatatag gcttcgccat
      661 cggatcaggg acggacatcg ccatcgaagc gagtgatgtc accttgatgc gaagcgatct
      721 acgcggcgta gcctcggcca tccggctggc ccgcacgacg atgcgcgtca tgaagcaaaa
      781 cctcttctgg gcatttttct acaatgtgct cgggattccc attgccgccg gcgcgttgta
      841 cccgttcacg ggtctgatgc tgagcccggt cttggcgagt gcggcgatgg cgttcagttc
      901 gatcagcgtg gtgatgaaca gcttgcggct acggacagcc cgcatcaact gaacgggttc
      961 gtgggcggct tgggaaagtc gtgggaacat ggctgatgct ccaagccgcc cagccgatcg
     1021 ttgacaaaag attttgtcgg agcgtagcct aacagtatgc actctgcgac ggcgcgacgc
     1081 ttactgacca tcgcggtcat tgttctcttt ttaggcttca gcatgaacgc ctttgcctgc
     1141 ttggtgccgt tatacggtgt accgaacatg gaggcgggtt gcccgtcagg gaacgagcag
     1201 cctccaagga cgttttgcga tgccttcaaa gctggtgtcc ctgcccattc gagctatatc
     1261 tatcccttcg cgatgcatct ctccgtgcct ctggttgcac tcgattcgtt ggggctctcg
     1321 tgcagcaccg aggtgctgac gagggacctc gattcttccg ctgactcacg cccacagaat
     1381 atcttcttca agacgacgat cctccgcatt tgatcccccg ttcgctctct gccccaaccc
     1441 gttccaggca cgactaagca gggcctttgg ccgagaagcg tgcggtgcgg acgattgccg
     1501 ccgcacgccc tttcggcata tcagcgggaa agggggtgat ctcagatgtg tcccattgaa
     1561 gaggcattag gcttttgtcc gtggggagcg gtcagtcaac tcctcacctt tctaggcttg
     1621 tcgtagtcta gtcaggggtc gtggtaaggc ggctaggcgt tgggtctagc cgtcttgccg
     1681 aagcgagggc ttgcccgcct gcatcggacc ggcccatatg agagttcccg tgaaccacgg
     1741 actgtaaggt ttgcgacgct agtccgtctg aagagccgag aacgtggttg ttaaggacac
     1801 agtcacttgc aaggagggta tgttatgaac acggcgacac atgcgactca tcaccatcat
     1861 catggccaga cccacgaggg caaggagacg ccgacgccac cactgaatcg gctcgccatt
     1921 agcgcgacgc tgcattgctt gacgggatgc gccatcggag aggtcctcgg tctcatgatc
     1981 gcaatggcct tgggatgggg taccgccgct tcgatcgtgc tggccatcct gctggccttt
     2041 gtcttcgggt atggatttac attggtccca ttagtccaag gaggcatgac gtgggcgaag
     2101 gcggcgtctg tcgcctttgc ctccgatacg gtctcgatcg cggtgatgga actcatggat
     2161 aacgccataa tgctcctgat tcccggagcc atggcagcgg gcctcaccga tgggttgttt
     2221 tggggcagcc tggtcctgtc gtttgccgtg gcattcgtgg tcacggttcc agtcaaccgt
     2281 tggctcatcg cccgtggtcg cgggcatgcc gtggtgcatg ctcatcatca ctgatgccgt
     2341 gcgcgaatcg gatgaacaag gaacgcggat ttttgggggg aagcaccatg aaacggacat
     2401 cgattacccg acgcgagatc ctgaaaggtg ccgcgacgct gggactgctt gccgccgccc
     2461 acgctctgaa accgattagg ctctgggcag gcaccgccgc cgaaccccct tcgccggaag
     2521 tcttgacggg cgacttgatc gatctgacga ttagcgaaca gatattccac ctcaacgggc
     2581 gagaggcgcc cgccgtcatg ataaatggga cgttacccgg acccctcatt cgcctcaaag
     2641 aaggtcaaca ggcaaccctt cgtgtcacga accgcttgcg agaagtcaca tccattcatt
     2701 ggcacggctt gctggtccca ccagaaatgg atggcgtccc cggagtcagc tttcgtggga
     2761 tcgaccccgg cgcgaccttc acctatcgct tcccgattca gcaaagcggg acctattggt
     2821 ttcacagcca ctccggcggg caggaattgc aggggatgta cgcgccgatg attatcgatc
     2881 cgtttgagcc ggagcctttt cagtacgaac gggactacgt ggtgatgctc tcagattgga
     2941 cctttgaatc gccgcctacc gtgctctcca atctcaaaaa gcaggcgggc tactacaatt
     3001 tccagcgacg cacggctggg gagttcctgg ccgatgtgcg ccgaaaaggt tggtgggcca
     3061 ctgtgcaaga ctatctggcc tgggaccgga tgcggatgga ccccaccgac tttgccgatg
     3121 tgacggggta cacctttacc tacctgatga atggtcggtc accggaaagt aactggacgg
     3181 gcctgtttcg tccccgagaa cgaatccgcc tgcgggtcat taacgcggcc gcgatgaccc
     3241 tgtttgacgt gcggattccg gggcttgaga tgacggtcgt gcaggccgat gggcaatcgg
     3301 tgcagcccgt ggtcgtggac gaatttcgca tcggtccggc ggagacatac gatgtcatcg
     3361 tggaaccgac tgatgacggc ccctacactg tgtttgcgga gaccctagat cgtagtggct
     3421 atgcgcgaag caccctggca acacgcgccg gtgcaatcgc cgctcttcct gaccgacgcc
     3481 cccgccccct ccgcaccatg gcggacatgg gaatgaatca tggactggcg tctcatagca
     3541 gtacggatgc tggcgtccat gacaggcacc tcccgacggg gaccatgccc tctcatacct
     3601 caccgaaccc ggggcaccaa cagcatggcc gaggcgccgg ggacgcgagt gctcatgaag
     3661 cgcggtcgga cgaagaaacg ggcgaccagc gcccatcgga aagaccaacc accgaacccg
     3721 tcccccacgg tcctgacgat catgggaccg gcaaccagat ggtggccgag tattcgtcga
     3781 accggatgca cgaaccaggg gcaggcttgg acgcaagcga acgacgtgtg ttgctctaca
     3841 ccgatttgaa aagcgtgcgc cccttcgacg atcaccgtcg gccagaccga gagattgagt
     3901 tgcatctcac ggggcacatg gaacggtaca tgtggtcgtt cgacggaaag aaatactcgg
     3961 aggccccacc cattcaattc cgctatggcg aacggatgcg cctgacgttc gtgaacgaca
     4021 ccatgatgga acatccgctg cacctgcatg ggatgtggat gtacttggaa aatggggctg
     4081 gcccgtactt gcctcgcaaa cataccgtca tcgtcaaacc ggctgagcgc ctgtccgtgg
     4141 caattagtgc ggatgcgccc ggccgctggg cctttcactg ccatttgcaa ttgcacatgg
     4201 aggcggggat gttccgagtc gtcgaagtct tggagccgac gacatcgtca tgaaggagcc
     4261 tggcccagtg ttattgctcg cccctctctt gttcctcgtt cagcttgtgt cttcgagtac
     4321 tatggccctc gccgagccgc aggaggtcaa gctgccgacg gttcactcgt ctgcttcggc
     4381 tcaccccgcg actcccgatc gcatgattaa tacccatttc acatacgcac ctgaaccctt
     4441 ggacctgtcc ttggtgaagg cgacgatacc gccgaagctt tcaccggggc gtgattggcc
     4501 gagtcccgtc gatgacgctg agcggcgcac gatggtgctg gtcgatgtgc tggaatatcg
     4561 cccgcagact cgaagcaccg gcagccagag cgattaccga tgggacatcg agggatggta
     4621 tgggggagat tacaaccggg tgtggttcaa gaccgaaggg cagcggaata ccgccttcaa
     4681 agcagactat gatgtcgatt tccaactgct ctacggacgg ttcatccaga aatactacga
     4741 ttttcagatt gggggtcgcg tcgaaaccca gacatttcgg ggggcgaatg tcacccgtgg
     4801 ccttgcggtg attggcatcg agggattggt cccctacagc tatcagattg aaccctcgct
     4861 cttcattgac cagaacgggg ccgtgtcggc gcgactcgcg gccacgaagg acctcttgat
     4921 gacgcaacgg ctcatcctcc aacttcgctt tgagacgaac gcggccctcc aacgagtcga
     4981 agaatttaca accgggaccg gcctcaacaa tgtcgagttc ggcgtccggc ttcggtatga
     5041 catccgacgc ggcttctcac cctatgtggg cgtctctctg gatcgcagct ttggagaaac
     5101 ggcgacgctc atccgccaag agggtggttc ttctagccag gtgagattcg cgctgggact
     5161 gcgaatgtgg ttttgaaggg aagtatcaac cttcctgttg ctcgcaggta caatccaagc
     5221 agccgtgttt cgtacaaatc ccgcacgcag cttcgaggct ggcacggagg gcctgccggg
     5281 gctcgatgta agcggacggt ggaggttatt cgcgtatgca ccatgaagag acggcaccga
     5341 gcattttgat aaattgaata catcatcgac cagggcgtct ccggaaaacc attgaggttg
     5401 tttgcttgag aagctcgaga tgcgtgagaa atgccctaga gtcgcatggc cagggccata
     5461 aaagagctgg cccctagata aggatccagg tatacgggca cacacgtgta tggttcacat
     5521 tttactcatt gaggataccg aggctgtacg gggtctgttt cgtgcgattc tagaacaagt
     5581 cggatatgtg gtgcgtgaag cgtcgacggg gtcagcggga atccgtctgt ttcatgagag
     5641 tccgtcggat gtcgtgatca ccgacatgta catgccagac ggcgacgggt ttgatgtcat
     5701 gcgtactttg cggcacgaag tcccggcgcc aaaaatcatc gtgatgtctg ggggattggg
     5761 caagggagag atactcgatg cggcccaatt actgggggcc gatctggtct tagccaagcc
     5821 agtgcccatg gacgagctat tgaaagcggt tgagacgctg atcggcacat cccgtctcta
     5881 gtgcagagtg tattgcgcac ccaacggaga cctggattga ggtgagaaga gattcgtcct
     5941 ttgaatggag cgagtcgtcg tcataatttg gtagtcggcc gccgcgcacg gatctggacg
     6001 gaagatcgcc ttaaaagggc cccagacaac aagtaaaatc aatgtcttac atgctgacac
     6061 attccaatga catacatggt tttacaatgg cttacaaata ggctgtccga tat
//
LOCUS       DAOQEW010000210         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4903_length_6105_cov_4.870083, whole genome shotgun sequence.
ACCESSION   DAOQEW010000210 DAOQEW010000000
VERSION     DAOQEW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4903_length_6105_cov_4.870083"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLS35_16705"
     CDS             complement(<1..1071)
                     /locus_tag="PLS35_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI20363.1"
                     /translation="MSRTWVTVGLLIMVSCLAQPSTAAWIDPLTPEERQGRNLYHYGK
                     AGIADPVAVNLQGVEGGVPPALFRCAGCHGHRGEGSQEGGLRVPPLTVSVLRRAREGT
                     SAGRPRAGYTDEALGKAITQGIDASGHALHPSMPRYQLTSDQVAKLIAYLTRLGEEAD
                     TDPGVSAEVVTIGAALPLTGPLAQIGQDVAATLRGALRRVNEQGGVYGRHIELIVEDS
                     RGDPAQTAQAVSRLIEIHQVFALVGSFEPAGGGRERFEQDQIPSIFPLTQSPQLSDPP
                     DPSIFYALPGFDLQFRSLMDFFASRTMPLTGTAQPRLALVHRNGDSNSEALRGALEQI
                     RHHALNLAMNYEYSTGRLAVLAL"
     gene            complement(1104..1685)
                     /locus_tag="PLS35_16710"
     CDS             complement(1104..1685)
                     /locus_tag="PLS35_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNI20364.1"
                     /translation="MMLRFVPAAMLLWLCGAHAAPAWSGVTPPSIPDVEVIDQDGVPH
                     RFYGDMVKGKTVVINFIYTTCKTSCPAATGLLSGVYHRLQAAERPVTLLSVTVDPVHD
                     RPTRLKAYAAQFGAGKGWYFLTGAPADVARILASLQVSASNKAEHPAIVVIGNEAAGI
                     WTRLYGEASVPMVMDGLDATAGPPALTPSGISQ"
     gene            complement(1699..3663)
                     /locus_tag="PLS35_16715"
     CDS             complement(1699..3663)
                     /locus_tag="PLS35_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HNI20365.1"
                     /translation="MRNILVGARGSLPAFLLVAAPAIVGAGETAPSHPVQRFEQNGLA
                     IELTVVPQDGPSETGAELTAGQTATVKVALMDAKSGSPVVGIRPKGWMVGRPSEMAAD
                     EHSCVEKIRRLAGGRLGARAEVDLNRYVIVTMNHDKTVSVIDPQVEFGATKLESLVTL
                     PGNGADWVLSPDQSYLYVTIPGEDAVAVIETRTRKLIQTIALDKGSRPGRILWQPDSR
                     RLWVGLDGTGQVAVLDGEGHRRLKTVTVGRGLHTLAAIPDGRFIYVTNSQSDSVSVID
                     TQAMAPVVSVPVGKTPVAVTYSSAARRLYVGGVNGGDIVAIDPSTHRLVGRIPMAAGV
                     VALATEPQGRFVFAVNQVESSLSVIDTATSTVTATAHVVKDPDQIVFTQRYAYVRGIE
                     SEKFTLLDLREARNGKLVPLDVQAGRQAPSAAPQELGVAPMIVPTPEGNAVMIANAPD
                     ATLHYYQEGMMAPSGTFSNYRRMPRGVLVLDRSLREVAPGVYQATVTLPHPGRFDVPL
                     LLDQPRMSHCFQVTVQESPASTATAPGPRPVRIQARFESDLVSAHVPASLSFDLLDAT
                     TQEPIVGLHDVQVLIFEPPGTWQQRRWAQEERAGRYVITQSFPHAGEYRLLVQSPSRH
                     LRFSDAPPVLLTVGPPMSVSTGSSEQALKP"
     gene            complement(3672..>6105)
                     /locus_tag="PLS35_16720"
     CDS             complement(3672..>6105)
                     /locus_tag="PLS35_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20366.1"
                     /translation="DALDSKGSIDAERTGREFNLAFADFAIVYDKDNQPVNPPSQIEG
                     GLRTPILPGRIPVPEGISTKDPGSQLINYRNEPIPLRIGTQDKDGHQFKQLPVDQEKG
                     DLAHAFSSTVHKDQKSVDERLFTEPSGKGRMPGDPATPLLRAYEGDHVQIRLIQGAQE
                     EQHVFTMHGSKWLAQPDSPNTGYVNAQTIGISEHFEFNDRVVDFMPFGRSNGHKSLGG
                     AGEHTSIDHLYSSAATDNLWDGQWGLMRVYSRTEDEPGLVRLPGNAEKTHDVLASAFM
                     QNLDELLDRESLDVSDDDAGNEAGQNLAQSDALEAFCQKSGQAQLTRSYEVEAWLAKD
                     LLPSGKLVYNEHFGIADPNAIIFVDASEKAMLRSGSKRPEPLILRARAGDCIRVTLTN
                     HLPAAMQDGPDYPETWSYNMVPPIVPGFSFNQVRSSSRVGLHPQLVDYNLLKSDGAHV
                     GLNDDSTVAPGVSKTYLWYAGDIVPNKKGTRIVGIPHEFGAIGLQDMGDVIKHASHGA
                     IGALLIEPQCSRWDTVPSTGNAEAVVTYWKPESASGRQGRHLKRQICPAEPKLDSGKL
                     YRFKEMVLLYQDNLSVQQYGQPVPNLRNGDDSEDSGQKGFNYRTEPLWARLGASAADE
                     PETMSQFDWSNVLSSTVPHFRCEADFANKKYCDPETPLFTAKAGEPVRFRVVHPGGHP
                     RQHAFTVFGHDWVPSPWIDASKAMGWNQDGLTRVGSAGGIGPGRDVNILTTAGGDCKV
                     PGDYLYRTQEGFMFGGGLWGIFRVEENPGLRWYQPAWAWAGNLFGYETNGVASADLCK
                     VHAANEAPIVQP"
BASE COUNT         1234 a         1923 c         1730 g         1218 t
ORIGIN      
        1 gagggccagc actgccagac gaccggtcga gtactcgtaa ttcatcgcga gattcaacgc
       61 atgatgccga atctgttcca gggcaccacg caacgcctca ctattgctgt ccccattcct
      121 gtgcacgagt gccagacgag gctgtgctgt ccccgtgaga ggcatcgtcc tggaggcaaa
      181 aaaatccatc aacgaccgga attgaaggtc gaacccaggc aaggcataga atatggaagg
      241 gtctggtgga tccgagagct gaggggattg agtcagcgga aaaatcgagg ggatctgatc
      301 ctgctcgaaa cgttcacggc cccctcctgc cggctcaaag ctccccacga gggcgaaaac
      361 ctgatgaatc tcgatcaagc gggagacagc ctgcgccgtc tgtgcaggat ctccgcgaga
      421 atcttctaca atgagctcga tgtgccggcc gtagacgccg ccctgctcgt tcacgcgccg
      481 tagtgcgccc ctcaaggtcg cggccacatc ctgcccgatc tgtgcgagtg gcccagtcaa
      541 gggaagcgcc gctccgattg taacaacctc ggcgctcacg ccgggatcgg tatcggcctc
      601 ttccccgagc cgcgtcagat acgcgatcag cttcgccacc tgatcggagg tcagctgata
      661 ccgcggcata ctcggatgta acgcatggcc ggaagcgtcg atgccttgag taatggcctt
      721 ccccagcgcc tcatcggtgt atccggcccg aggtcgtccc gcgctggttc cctcgcgcgc
      781 ccgcctgagc accgatacgg tgagtggcgg cactcgcaac ccaccttcct gtgatccttc
      841 cccacggtga ccatggcatc ccgcgcaccg aaacagggcc ggcggaaccc ctccctcaac
      901 cccctgcaaa tttacggcaa ccgggtccgc gattccggct tttccataat gatacaggtt
      961 gcggccttgg cgctcctccg gcgtgagggg gtcgatccaa gctgcagtgg agggctgcgc
     1021 caggcaactc accatgataa gcaggccgac ggtgacccaa gtcctgctca cgacggggca
     1081 tcgatgtacc atggtgcgat gacctattga gagatgccgg aaggcgtcaa ggccggcgga
     1141 cccgctgtgg cgtcaagccc gtccatcacc atcggaacag aggcctcgcc atacaggcgc
     1201 gtccatatcc cggccgcctc attcccaatg accacgatgg ctggatgctc tgccttgttt
     1261 gatgcagaga cctgcaggga tgccagaatg cgggccacat cggcaggtgc gccggtgaga
     1321 aagtaccatc ccttccctgc gccaaactgg gccgcatacg ctttaagccg tgtcggccgg
     1381 tcgtgaacgg ggtcgaccgt cacagacagg agggtgacag gacgttccgc ggcttggagg
     1441 cggtggtaca ccccactgag caagccggtg gctgcgggac aactcgtttt acaggtcgta
     1501 tagatgaagt tgatgaccac ggttttgccc ttcaccatat cgccgtaaaa acgatgtggg
     1561 acaccgtcct ggtcaatcac ctcgacatcg gggatggagg gcggggtgac accggaccag
     1621 gccggcgccg catgagcgcc acacaaccac aagagcatcg cggctggaac gaagcggagc
     1681 atcatagatc agacgccctc atggcttgag cgcctgctcc gaagagcctg tggacacaga
     1741 catcggcggc ccgacggtga gcagaacagg cggagcatct gagaagcgga ggtggcggga
     1801 tggcgattgc accaacagcc ggtattcccc cgcatgcgga aaagactggg tgatcacata
     1861 ccggccggct cgttcttctt gcgcccatcg ccgctgttgc cacgtgccgg gcggctcgaa
     1921 gatcaacact tgcacgtcat gaagccccac gatcggctcc tgagtggtgg catccagcaa
     1981 gtcaaacgac aaggaagcgg gaacgtgcgc cgacacgaga tcactttcga accgggcttg
     2041 tatgcgtact ggtcgagggc ctggagccgt cgctgtggat gccggtgact cctgaaccgt
     2101 cacttgaaaa cagtgggaca ttcgaggctg atcaagcagc aggggaacgt cgaatcggcc
     2161 agggtgcgga agggtcacag tcgcttgata gacaccgggg gccacttctc tcaagcttcg
     2221 atcgagcacg agaacgcctc gcggcatccg acgataattc gagaaggttc ccgacggagc
     2281 catcatgcct tcctgataat aatggagcgt cgcatccggt gcattggcaa tcatcacggc
     2341 gttgccttcg ggcgtcggaa caatcatggg cgcaacacca agttcctgtg gcgcggcact
     2401 cggcgcttgc ctcccggctt gcacatcgag cgggaccagt ttcccatttc gcgcctcgcg
     2461 caggtcaagg agcgtaaatt tttctgactc gatgccacgc acataggcgt atcgctgtgt
     2521 gaacacgatt tgatcgggat ctttgaccac gtgtgcggta gccgttacgg tactcgtggc
     2581 ggtatcgatg acagacaggc tgctctcaac ctgattgacc gcaaagacaa accggccctg
     2641 aggttcggtg gccaaagcaa ccacgcccgc ggccatggga atccggccca cgaggcgatg
     2701 cgtcgacgga tcgatcgcta caatgtctcc gccgttgaca ccaccgacat acaaacggcg
     2761 ggctgcactg ctgtacgtca ccgcgactgg tgttttgcca accggcaccg acaccaccgg
     2821 cgccatggcc tgcgtgtcga tcacagacac gctatcgctc tggctgttcg tcacatagat
     2881 gaatcgaccg tcgggaatcg cggccaatgt atgcagacct cgtcccaccg tgacggtctt
     2941 gagccgtctg tgtccttctc catcaagcac cgccacctga cccgttccgt ccagccccac
     3001 ccagagccgg cggctgtcag gctgccagag aattcgtccc gggcgggatc ccttgtcgag
     3061 cgcaatagtc tggatgagct tccgtgttcg agtctcgatc accgccacgg cgtcttcgcc
     3121 cggaatcgtg acatagagat aactctgatc cgggctgagg acccaatccg ctccattgcc
     3181 aggcagcgtc acgaggcttt ccagcttggt cgcgccaaac tccacctgcg gatcgatcac
     3241 cgataccgtc ttgtcgtggt tcatcgtgac aatcacgtat cgattcagat cgacttcggc
     3301 acgggctccc aatcgtcctc cggccagccg gcggatcttc tcgacgcagc tgtgttcatc
     3361 cgccgccatt tccgaaggcc gacccaccat ccaccccttc ggccttattc ccaccactgg
     3421 agacccgctt ttcgcatcca tcagcgcgac cttcaccgta gctgtctggc cggcagtcag
     3481 ttctgcccct gtctcggaag ggccatcctg aggaaccacg gtcagctcga tggcaagccc
     3541 attctgctca aaccgttgca caggatggga tggcgcagtc tctccggccc cgactatcgc
     3601 cggagcggca accagcagga aagccgggag acttccccta gccccgacaa ggatattgcg
     3661 catggcttgt ctcatggctg cacaatcggc gcctcgttcg ccgcgtgaac tttgcacagg
     3721 tcggccgagg ccacaccgtt cgtctcgtac ccaaacaggt tgccggccca agcccaagcc
     3781 ggttgatacc agcgtaatcc gggattctcc tcaacccgga agatccccca caatccaccg
     3841 ccgaacatga acccctcttg cgtgcgatag agatagtcgc ccggaacctt gcagtccccg
     3901 ccggccgttg tgaggatatt gacgtcgcgg cccggaccaa tcccgccggc tgatccgacc
     3961 cgcgtcagac catcctgatt ccagcccatg gctttggacg catcgatcca gggactcggc
     4021 acccagtcat ggccgaacac cgtgaacgcg tgctgtcgtg gatgccctcc cggatgcacc
     4081 acccggaacc gcaccggctc tcctgccttc gcggtaaaga gcggcgtttc gggatcacaa
     4141 tatttcttgt tcgcaaaatc cgcctcgcag cggaagtgcg gcaccgtcga gctcaagacg
     4201 tttgaccaat cgaactgact catcgtctcc ggttcgtcgg ctgcgctggc ccctaatcgc
     4261 gcccagagcg gctccgtgcg gtaattgaag cctttctgcc ccgagtcttc ggagtcgtcg
     4321 ccattccgca aattgggaac aggctggcca tactgctgga cgctcaggtt atcctgatag
     4381 agcaacacca tttctttgaa tcgatacaac ttcccggagt ccagcttggg ctccgcgggg
     4441 cagatctgcc gtttgagatg cctgccctgc cggcccgagg ccgattccgg cttccaatag
     4501 gtcaccaccg cttcggcgtt gccggtcgac ggcacagtgt cccagcgtga acattgcggt
     4561 tcgatgagta atgcgccgat cgcgccatgt gacgcatgct tgatgacatc ccccatatcc
     4621 tgcaagccaa tggcgccaaa ctcatgggga atgccgacga tgcgagtccc tttcttgttg
     4681 ggcacaatgt cgccagcgta ccagaggtag gtcttgctga cacctggcgc gacggtggag
     4741 tcgtcattga gtccgacatg cgcgccgtca ctctttagca ggttatagtc caccaactgt
     4801 ggatgcagtc caacccggct cgacgaacgc acctgattaa acgagaaccc agggacgatc
     4861 ggtggaacca tgttgtaact ccaggtctcc ggatagtcgg gaccatcctg catggcggca
     4921 gggagatggt tggtcagggt gacacggata caatcccctg ccctggcacg aagaatgagc
     4981 ggctccggcc gcttgcttcc tgaccgcagc atggctttct cactagcgtc tacaaagata
     5041 attgcattgg ggtccgcaat accgaaatgc tcgttgtaga ccaacttccc actcggcaac
     5101 aggtccttgg ccagccaggc ttccacctca taggaacgag tcaactgagc ctgcccgctt
     5161 ttttggcaga aggcctccag cgcatcgctc tgtgccaggt tctgaccggc ttcatttccc
     5221 gcgtcatcat ctgagacatc cagtgattcg cgatccaaca gctcatccag gttttgcatg
     5281 aaggctgacg ccaggacatc gtgcgtcttc tccgcattgc ccggcaaacg aaccagccct
     5341 ggctcgtcct cggtacggga atagacacgc atcaatcccc actgaccatc ccacaaatta
     5401 tccgtggcgg ctgaggagta gagatggtcg atcgatgtgt gttcgcctgc tccgccgagc
     5461 gatttgtgtc cgttgcttcg cccgaacggc ataaagtcca ccacgcgatc gttgaattca
     5521 aaatgctcgg aaatcccgat cgtctgcgca tttacgtagc cggtgtttgg actatccggc
     5581 tgggccagcc atttggagcc atgcatggtg aacacatgct gttcctcctg ggcgccctga
     5641 atcagccgaa tctggacatg atcgccttca tacgcccgca ggagcggtgt cgccggatca
     5701 cctggcatac gtccttttcc gctaggctcg gtaaacagac gctcgtcgac agatttctgg
     5761 tctttgtgaa ccgttgaact gaacgcatgc gccagatcgc ctttctcctg atcgacgggc
     5821 aattgtttga actgatggcc gtctttgtcc tgtgtgccga tgcgcagcgg aatcggttcg
     5881 ttccgataat tgatcagctg ggaaccgggg tctttcgtgg agatgccctc cggcacagga
     5941 atacggccgg ggagaatagg cgtccgtaaa ccaccttcga tttgactcgg tgggttcacc
     6001 ggttgattgt ctttgtcgta cacaatcgca aaatcggcga aggccagatt gaattcccgc
     6061 ccggtccgtt cggcatcgat gctgcccttc gaatcgagcg cgtca
//
LOCUS       DAOQEW010000211         6066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4951_length_6066_cov_8.551822, whole genome shotgun sequence.
ACCESSION   DAOQEW010000211 DAOQEW010000000
VERSION     DAOQEW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4951_length_6066_cov_8.551822"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..1158
                     /locus_tag="PLS35_16725"
     CDS             268..1158
                     /locus_tag="PLS35_16725"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNI20367.1"
                     /translation="MSLPSRIALELFTAKQAWSAGNEGKARVCARRAVAAAAESWFAH
                     RGEQVAQADAMTYLRRIQQQGTFPLPVRQAAERLTTAVTRRHEAPFTTDPFTDASLII
                     SHLAADSSSEGHADVSPEREDDLQSRPGAGRILIILIMGVSGAGKTTVGRRLAEVLGW
                     RFLEGDDLHPIANIEKMRSGHPLTDADRRPWLERLHTAIADQVRMNQPAVMACSVLKA
                     RYRTVVEEGFGHALRLVYLKGTADVFRERLAGRRNHFMRPELLDSQLAILEEPADALV
                     VDAALPPDEIILRIRQGLAV"
     gene            complement(1195..1554)
                     /locus_tag="PLS35_16730"
     CDS             complement(1195..1554)
                     /locus_tag="PLS35_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNI20368.1"
                     /translation="MNEQFARSLAEAWIDAWNRHDLEAILHHYASDVEFTSPFVPALS
                     GDPSGTLHGRDRLSAYFQKGLHTYPDLHFDLLQVLTGIDSLLLYYRSVKGLLAGEMMT
                     VNAEGLIQTVRVHYVKE"
     gene            1664..2128
                     /locus_tag="PLS35_16735"
     CDS             1664..2128
                     /locus_tag="PLS35_16735"
                     /inference="COORDINATES: protein motif:HMM:NF021425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HNI20369.1"
                     /translation="MSAPISQQTSLAPIALFKLVKGLLVLLAGSGFLRWIDPEIDSGL
                     SPVLDALHLNIHTRLLHALAITVDSLPRHSLLLMSLVSLGYAALLFVEGFGLWSETSW
                     AAYLTVVSTCVLLPGEFHAVVREWSMAGLAVLAVNIAIVGYLVRRLADETLR"
     gene            2235..2627
                     /locus_tag="PLS35_16740"
     CDS             2235..2627
                     /locus_tag="PLS35_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20370.1"
                     /translation="MVEVNSMYQPRMRNDDIKAQIDKLEGLAHVTAVHPTFDTFDEET
                     ETILTRIFGGSHPYVESYKYATLGEAEAIVNLPESAQEPLTRDTPKKGLQQRRQVLQS
                     ILTDLQGLEAEEAEALTGEDHEDPPGPS"
     gene            complement(2782..3594)
                     /locus_tag="PLS35_16745"
     CDS             complement(2782..3594)
                     /locus_tag="PLS35_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20371.1"
                     /translation="MPAFLTATARSGRLGIASVGGLCLAISLTAGPLLWAGSSSPTKP
                     GKGPQEAEQGRVLFNGKGLCSTCHGVDGHKGQLPPHLTQHIRDNIAHLDPAPADLRQA
                     RTLTLTTDKQRFEIIRHGHLRTAMYPISKDTISDEEILALLPYLASIRGAVSPPASVP
                     ASSFSLRGDANNGRQLYHELGGCAICHGIEGHLDSRPPMSADLISRLKALPHPPADLR
                     HPSSLTAQSDEDLYRSIKLGHPGTAMYPKTLLRDEDIRDLIAYLAVLRGGSQ"
     gene            3744..4484
                     /locus_tag="PLS35_16750"
     CDS             3744..4484
                     /locus_tag="PLS35_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNI20372.1"
                     /translation="MGSMIHTAMNMVDHRHCLALSGCFRGKLCEQLMNRPGRHMPKGS
                     RVYGPGDPAQSVFYLRRGFVKLTSLTEDGRELILRLHQTGEVFGELCHCTGERREQAI
                     AMEDSEIVELSFDEFIAHLQSNRPAFLLFLSNVAQQLSAAYDQIQTLSFNSTMERLVR
                     TLGRLADEFGEPDGEWIRLTHYFRQDDLAQMIGARREVVSTLLNQLREQGLITYARKD
                     GVLLRRAELDRFLGSTAQSSASLADSGF"
     gene            4618..5268
                     /locus_tag="PLS35_16755"
     CDS             4618..5268
                     /locus_tag="PLS35_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20373.1"
                     /translation="MRKVIHACSVLMLSVATVACAHTTDTPAPVASSAAGHQAKVAET
                     KAALRDLWLGHILSIRNVAVATMDKNAAARSAAEADVVANAQQIARSIEPFYGKPAAD
                     QLFTLLAGHYQGIREHLDATVAGSTSQQDAAVKALTSNAAEIATFLSGANPNLPKDTV
                     MGLLMAHAAHHLTQFQQLKDGEYAREAETWKGMKQHIYVVADALTQALAAQFPAKF"
     gene            5655..>6066
                     /locus_tag="PLS35_16760"
     CDS             5655..>6066
                     /locus_tag="PLS35_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20374.1"
                     /translation="MGRIRHCVVSGKVLLLALVLSVAPAICQAAEWVHEGMADEGKMT
                     FGFRVGPSFMTQSSGLSTTGPALNFQGMYGINKWFRFGMMLEWENHSIDGPRNGSLNT
                     ITLLPVNLEYRPGHFGALIPYVTSGIGVNINTKDQ"
BASE COUNT         1180 a         1691 c         1868 g         1327 t
ORIGIN      
        1 agtcatcatg ctgtgttcag ccctgacaga ggtctttgtg gcacgtgctg ccgtccgctc
       61 gtcagtgcgc agcgagtatg cctgcccggt cttctgaggg gaataggggt ttccctgaaa
      121 gaggggggaa ttatcactgt gacgagcaga tgttccgttc tgaacgccag gctccgcctt
      181 cgcatcagcc gatgggtgga gatgtgtgtg gcatcatcag atggcctcat tgacagtttc
      241 aacttcagcc acgtagcgta acggcccatg tcgctgccat ctcgcattgc gctggaactc
      301 tttactgcga agcaggcctg gagtgcgggc aatgaaggga aggcgcgagt ctgtgcacgg
      361 cgggcggtcg ctgccgccgc cgaatcctgg ttcgctcatc gaggtgaaca ggttgcacag
      421 gcggatgcga tgacctattt gcgtcgcatt cagcagcaag gcacctttcc tcttcctgtc
      481 cgccaggcgg cggaacgcct cacgactgcg gtgaccaggc gccacgaggc accgtttacc
      541 accgatccgt tcaccgacgc ttctctgatc atttcgcatc ttgcagccga ttcatcgagt
      601 gaagggcatg ctgatgtttc tccagagcga gaggacgatc tgcagagccg gcctggagca
      661 ggtcgcattc tcattatcct gatcatgggc gtctccgggg cagggaaaac gacggtcggc
      721 cgccgcctgg cagaagtctt aggctggcgg ttcctggagg gcgacgatct ccatcccata
      781 gccaacatcg agaagatgcg gagtggacat cccttaaccg atgccgatcg acgcccttgg
      841 ctggaacggc tgcatacggc cattgccgat caggttcgga tgaaccagcc ggctgtgatg
      901 gcctgttctg tgttgaaggc gaggtatcgg actgtagtcg aggaggggtt cggccacgcc
      961 ctgcgactgg tctatttgaa aggcacggcc gacgtatttc gtgagcggct cgccggtcgc
     1021 cggaaccatt tcatgcgacc ggaactgctt gatagccagc tggcgattct ggaggagccg
     1081 gccgatgcgc tggtcgtcga tgccgctctg cctccagatg aaatcattct ccggatcaga
     1141 caaggcttgg cggtctaacg aggcgagcac cgtcatctgg ttccctcccg gcctctattc
     1201 tttgacgtaa tggacccgca cggtttggat caatccctcg gcgttcacgg tcatcatttc
     1261 acctgccagc agtcccttga cgctacggta atagagcaac aggctgtcga tgcctgtcag
     1321 aacctgtaac aagtcgaaat gcaggtcggg ataggtgtga agtccctttt ggaaataggc
     1381 gctcaaccgg tcgcgtccgt gaagtgtgcc ggacgggtcc ccggatagcg ccggcacaaa
     1441 cggactggta aactccacat ctgatgcata atgatggaga atagcctcga ggtcgtgccg
     1501 gttccaggcg tcgatccacg cttcggccag gcttcgtgca aactgttcgt tcatcaccgt
     1561 gaacctttca ttgagagcct ttggtggtga ctagcctagg ggaacggcta cctgatgagc
     1621 aagggccgat cctcgtcagg cggatgaaag tggcgctatg cttgtgagcg cgccgatatc
     1681 gcagcagacg agcctggctc ccattgccct cttcaagctg gtgaaagggt tgttggtcct
     1741 gctggcggga tcaggctttc tccggtggat cgatcctgaa atcgattccg gtctctcgcc
     1801 cgttctagac gccctgcact tgaacatcca cacacggctg ctacatgcgc tcgccatcac
     1861 ggtggattcg ttgcctcggc acagtctgtt gttgatgagt ctcgtcagcc tcggctatgc
     1921 ggccctcttg tttgtcgagg ggttcggtct ctggagcgaa acctcctggg ctgcctatct
     1981 gacggtcgtg tccacgtgtg tcctcttgcc cggggaattt cacgccgtcg ttcgcgagtg
     2041 gtccatggcg gggctggcag tgctggcggt gaatattgcc atcgtgggat acctggtgcg
     2101 caggctggcg gacgagacat tgcgttgacg ggcctggttt gtgcgtgttc caacttcgat
     2161 tctcggggaa ttcctaggtg gcattccgtt ctggaggcga tataccaaaa ccatcagacg
     2221 agaggagaca tgccatggtc gaagttaact ccatgtatca acctcgaatg aggaacgacg
     2281 atattaaggc acaaatcgat aagcttgagg ggctggctca tgtcaccgct gtccatccca
     2341 cctttgacac attcgatgaa gagaccgaaa cgatcctcac cagaatattc ggcggctctc
     2401 atccctacgt cgagtcctat aaatatgcga cgctggggga agccgaagcg atcgtcaatt
     2461 tgcctgaatc agcgcaggag cctctgacgc gcgatacgcc gaagaagggc ctgcagcagc
     2521 gccgtcaggt gctgcaaagc atcctgaccg acctgcaggg gcttgaagcg gaagaggccg
     2581 aagcactgac gggagaagac cacgaagatc ctcccggccc aagctaatgg tcctataccg
     2641 gttattccgg gcatgggggc ggtgaccgcc ggtgagttgt gcgtaaaggt ggtcgctgcc
     2701 tccgcgagcc gcttccgaac gactgtgccc ctcccacttc cctcgtgatc gcactcacct
     2761 ctggctccat tcctccttca gctattggct tcctccacga aggacagcaa gataggcaat
     2821 caagtcccgg atatcttcgt cccgcaggag cgttttcgga tacatggccg tgcctggatg
     2881 gccgagtttg atggatcgat agaggtcttc gtcactttga gcggtcaggc tggacggatg
     2941 acgcaggtcg gccggagggt ggggaagcgc cttcagtcgt gatatcaaat cagccgacat
     3001 cggggggcgt gaatccaagt gtccctcgat gccatggcag atggcgcagc cgccgagttc
     3061 gtggtagagc tgccgcccgt tgttcgcatc gcctcttaag gaaaaggaag acgcgggcac
     3121 ggaagcggga ggcgatactg caccgcggat ggacgcgaga tagggcagca acgcgagtat
     3181 ctcctcatcg gagatggtgt ccttcgagat cggatacatg gcggtgcgca gatgcccgtg
     3241 ccggatgatc tcgaatcgtt gcttgtcggt ggtgagggtg agcgtcctgg cctgcctcag
     3301 atcggcggga gcgggatcga ggtgtgcaat attgtcccgg atgtgttgag tgaggtgcgg
     3361 cggcagttgg cccttgtgtc catcgacacc atggcaggtg gagcagaggc ccttgccatt
     3421 gaagagtacc cggccttgct ccgcttcttg cggccctttg cccggcttgg ttggagacga
     3481 ggacccggcc caaagcagtg gaccggcggt cagtgagatc gcgagacaga ggcccccgac
     3541 actggcaatt ccaaggcggc ctgatcgagc cgttgctgtc aagaacgccg gcatggtcga
     3601 tgtccggatg atgtggtctt taaatccaac aatctccatc ataattgaag gcgacggtgc
     3661 agtgctagaa ccgatggcca caccgtgatg ggacctttgg ggggctggtc agacgcgtgg
     3721 cgaccccgct acaatgtctc tcgatgggta gtatgattca tacggccatg aatatggtgg
     3781 accaccggca ctgcctcgcc ttgtccggtt gtttccgggg caagctctgc gagcaattga
     3841 tgaatcgacc ggggcggcac atgccgaagg gcagtcgcgt ctacgggccg ggtgacccgg
     3901 cgcagagcgt cttctatctt cgtcggggat tcgtgaagtt gacctctctc accgaggacg
     3961 ggcgtgaatt gatcttacgc ctgcaccaga ccggtgaagt gttcggcgaa ctgtgtcatt
     4021 gcaccggcga gcgccgcgaa caggccattg cgatggaaga cagtgagatc gtcgaactga
     4081 gtttcgatga atttattgcc catttgcaaa gcaatcggcc ggcgttcctc ctgtttcttt
     4141 ccaatgtcgc ccaacagctg tcagcggcgt atgatcagat tcagactttg tccttcaata
     4201 gcacgatgga gcgcctggtg cgcacgctgg gacggttagc cgacgaattc ggcgaaccgg
     4261 acggcgagtg gattcgtctg actcactatt tccggcagga cgatctggcc cagatgatcg
     4321 gcgcacgacg ggaggtggtg tcgaccctcc tgaaccaact gcgtgagcag gggctgatca
     4381 cctacgcgcg gaaagatggg gtactccttc gtcgggccga actcgaccga ttcctggggt
     4441 ctactgccca atcatccgcc agcctcgccg attccggctt ctagcgccgc atatttctcc
     4501 tccagtgtta ccgcgctaac ttacagggtc gctcccgttc tctatccttg cccacgagtg
     4561 caacagccaa tgtcggctgt ccggcgctgc ctcactattt ttacaaggag agaagacatg
     4621 cgtaaggtga ttcatgcctg ttcagtgctg atgctcagtg tggccaccgt ggcctgtgcc
     4681 catacgacgg ataccccggc tcctgtggct tcttcagcag cgggccacca ggctaaggtg
     4741 gcggaaacga aggcggcgct tcgtgatctc tggttggggc atatcctgtc tatccggaac
     4801 gtggccgtcg cgacgatgga taaaaacgcc gcagcgcgga gcgcggctga agccgatgtg
     4861 gtggcgaacg cccagcagat tgcccggtcg atcgaaccgt tttatggcaa gccggcggcg
     4921 gatcaactct ttacattatt ggccgggcat taccagggga ttcgggaaca tttggatgcc
     4981 accgtcgccg ggtcgaccag ccagcaggat gcggccgtga aggcgctgac ctccaatgcg
     5041 gctgaaattg ccaccttcct cagcggggcc aaccccaacc tgccgaagga cacggtgatg
     5101 ggattgctca tggcccatgc cgcccaccat ctcacgcaat ttcagcaact gaaggacggc
     5161 gagtatgcgc gtgaggcgga aacctggaaa ggcatgaagc agcatattta tgtcgtggcg
     5221 gatgcgctga cgcaggcctt ggccgcgcag tttcctgcca agttttaagg catcctcgag
     5281 aagcgatacg acggttcctc gtctcattcg agactcgtgc tctttcggag gctggactca
     5341 ggcggcccgc agcggattag ttgcgcgggc cgcctgatgg ataactcgtt ccacggggcc
     5401 cgcgtctcct ctcgatctgt cgatggttcc actctcgcgc gtcggaccgg aggtcccgcc
     5461 ggttcactgc ttggcgctac gtagttcccg gggctattgc gtcatctcga cgatggtctg
     5521 tcgtcgcaag gtgcgacaat cattggaacc attttatttc tcgtcatcgg cgcagcgcat
     5581 attgaaaatc cggaagccac ttgcctagag tgcgcagtcg aaacatccgt ccaatctcat
     5641 cacaggagga ccgtatgggg aggattcgtc actgcgtagt gtcgggcaag gtgttgctgt
     5701 tggccctggt gttgagtgtg gctcccgcca tttgtcaggc ggctgaatgg gtgcatgagg
     5761 ggatggccga tgaaggcaag atgacgttcg ggtttcgggt gggtcccagt ttcatgacgc
     5821 aaagttccgg cctctccacg accggtccgg cgctcaactt tcagggtatg tatgggatca
     5881 ataagtggtt ccgcttcggg atgatgctcg aatgggaaaa ccatagcatt gatgggcccc
     5941 ggaacggatc gctcaatacg attaccctgt tgccggtcaa tctcgagtat cggccgggac
     6001 atttcggagc cctgattccg tatgtcacca gcggtatcgg cgtgaacatc aacaccaaag
     6061 atcagg
//
LOCUS       DAOQEW010000212         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4957_length_6063_cov_8.910786, whole genome shotgun sequence.
ACCESSION   DAOQEW010000212 DAOQEW010000000
VERSION     DAOQEW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4957_length_6063_cov_8.910786"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLS35_16765"
     CDS             <1..298
                     /locus_tag="PLS35_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20375.1"
                     /translation="EDAGHIAIVIRVLADGIVVGGITYVLDPALGDRDDAKKCCEPCI
                     DDRCKPCRPPCDEPKPECPPEPCEPKVCCEDSARRVCVQVELGPCEPKPHKSAT"
     gene            complement(960..1154)
                     /locus_tag="PLS35_16770"
     CDS             complement(960..1154)
                     /locus_tag="PLS35_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20376.1"
                     /translation="MDHNRLLMAGSSQGGVQVGRRKPVTQGDPAREILAGSPTAGDGR
                     VREVTNGSFGAAKLVESLGT"
     gene            1487..3274
                     /locus_tag="PLS35_16775"
     CDS             1487..3274
                     /locus_tag="PLS35_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNI20377.1"
                     /translation="MREAGIQLRPVSALLTDAAGHPARYWIPAYQRGYRWTPLQVTQL
                     LDDIWEFIQYSEGGPRQAFYCLQPLVIKARPQGDYEVVDGQQRLTTLYILLTYLGDMA
                     KMLGKCRFQLRFETRGESNEPFLAHIDPTRAGENVDFFHICEAYRAIEQWFSGRDGMH
                     KLKLLQHVLNDDEAGRNVKVIWFELAAKDDPVAAFTRLNVGKIPLSNDELIRALFLRK
                     AAAADAGPGQALRIAYEWDQLEKALQRDAFWYFLSNAPPLGSNRIGMLFDLVATSGMS
                     ALLKQDAYGVFHVFSERLKASSATTEWLAVKQTFMALDEWYEDRELFHVVGFLIQQGE
                     TINALLEAAVTRRKAEFAGELRRRAFALAIGADLAALDDGQLTARIRERCEGVSYARD
                     PGAIRTLLLLFNLATLLENPRSNLRFQFDSFKRENWDIEHVRAVAARRPERHNERLEW
                     LGHCQGYLQTREDGQALAQAIEDFVALPQRSAVDALFDPLYEDVLIYFHESDEGEENG
                     LSNLTLLDRSTNRSYKNAVFALKRQQLLELDQSGIFVPLCTRNVFLKCYSPQVDNAMF
                     WSQEDANAYGDVIVETLRRFFCSGREVQV"
     gene            3271..5667
                     /locus_tag="PLS35_16780"
     CDS             3271..5667
                     /locus_tag="PLS35_16780"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNI20378.1"
                     /translation="MSNNGAQLIFSELLTRHGRIQVPLIQRDYAQGRADQQEVREEFL
                     AALHEALRLPPEAESLPLNLDFIYGSVEGQVASCFQPLDGQQRLTTLFLLHWYLAWRD
                     GRGDEFRALCTDEAGSRFSYHVRPSSSEFFDALVAFQPRRSPDDVTAVSVLIIDQPWY
                     FRNWRLDPTIQSALVMLDDLHKYFRSEQGLYARLIDTERPAITFQLLDLKHFGLSDDL
                     YIKMNARGKPLTPFETFKARYEQTLKDLFPKEQRVLDGQYVSMAEFFARRMDTSWADF
                     FWPHRDPNSNVFDAAVMNVFRAVILITRSPQADGFGEDVNLLRNRFRKNSYAFFHQRD
                     WLDRPFSEMLAVLLEAWSAAGSDIGMQLPDGRYFNEKAVFDKLLSDPTTLGYEDIVQL
                     AAYGQYLKTHGDKFESTDFQAWMRVVFNLSVNTEYNRPADLQRSLAALNTLAPHMTSI
                     LDYLARADSEITGFNQQQVAEEKLKAKLLIADNTWRVLIEQAEGHGYFRGQIGFLLAF
                     SGVSVHPAAEGEEPWTHETTLRLQHEFKGVLQQASAMFGDSGLNSLPDYRWERALLAL
                     GNYLLAARRNDSFLVNAQAEPASWKRLLRGAWSGSPQAAVLCSLWKRLQGTANLEAQL
                     DAVIAEASGIEDWRAALIATPAAIGYCHQRMIRHAENARTYLLKKLQMNGAHAELFTY
                     CLYAQTLQSADREGRLQVLTPYYEETNTAEEEPHVRLRGHHGDRLLIFVIEFDYVQYE
                     IKLLEPHPEFLPDPLLALFQKIGFALTGTHLVKHSTIDNIESDIFELDRHLQIMRRNV
                     "
BASE COUNT         1280 a         1686 c         1785 g         1312 t
ORIGIN      
        1 ggaggacgcc ggccacatcg cgatcgtgat ccgggtgctc gccgacggca tagtcgtcgg
       61 cggcattacc tacgtgctcg accccgcgct cggtgaccgc gacgacgcga agaagtgctg
      121 cgagccctgc atcgacgacc gctgcaaacc ctgccgcccg ccgtgcgacg agccgaagcc
      181 cgagtgcccg cccgagccgt gtgagcccaa ggtctgctgt gaggacagtg cgcgccgcgt
      241 gtgcgtgcag gtcgagctcg gcccgtgcga gccgaagccg cacaagtcgg ccacgtagaa
      301 tcccgcgcat aagcatatcg acgcggatct tgcagtaacg atccaccggc aaagccggtg
      361 gctttcgatg gctggcccct caaaggggcc ctttcgtcat cggggaaaag cagccggcat
      421 caaccggcgg tgcgtaccga caggccgctt cgccccgaag atcgggtacc gtccgccccc
      481 agggaaggtg ctcatagttg cgcgcatagg ggccgtgaga gtgaattcgg ggatcggaac
      541 agcagtctga ccaaccgagt ttaggttgtc tggccgataa ctgttttaaa ccagccgttg
      601 agcgcgggtt gggtcaacgg tggctccgtc cgagttgctc gcgtaggcta ccaggcaggt
      661 ttcagagtga agctgacgct caaatgacaa ctatgcggaa tcaccggccg gctggttgtg
      721 gccgactgtg tgagatcact aacgacagct ttgcggcagc gaaactcgcg gaaggctgtt
      781 gtgcctgaac ggcagcttca gggaagtccg catgaccgct cagggtcgaa acgactcacc
      841 agccagtggc agctttcggg aagcgaacct tcaacgtcgg ctttccggcg gtgagtccga
      901 agagttgtcg gtcgccaccc gaccccgagc ggtcgttgcg acttccgcaa agctgccggt
      961 caggtaccca gggattccac gagtttcgct gctccgaagc tgccgttcgt gacctcacgc
     1021 acccggccat caccggccgt tggagatccg gccaggattt ccctcgctgg atcgccttgg
     1081 gtgaccggtt tacggcggcc aacttgaacc ccgccttggc tcgacccggc catgagcagc
     1141 cggttgtggt ccaaagagcc tgcccattcg gctggcttct caatttaggg tagctgccgc
     1201 tcacccaact ggcacataca tcccacgttg gcctgatcta ccctaaaacc tagtttgacc
     1261 ggacctaccg acaacatctg aaacagctaa agctcaaagg gtctgcaaca gggctccacg
     1321 gccaagtcta gtatgattgc tgggtgatgt gcgcccgcac gttctgagat agcaagccag
     1381 cacattactt cctttcgctt tccgtgaaat gtccggcaca gcgtcatctt gggctagcct
     1441 catgcctgtc atacaacggg atccaccatc gagggttgag atcgttatga gagaagccgg
     1501 tattcaatta cgcccggtta gtgccctgct cacggatgcc gccggccatc ctgcgcgcta
     1561 ctggattccg gcataccagc gaggttatcg ctggacgccg ttacaggtca cccagttgct
     1621 cgacgacata tgggagttca tccagtacag cgagggaggg ccgcgccaag ctttttactg
     1681 tttgcagccg ctggtcatca aggcccgccc gcaaggtgac tacgaggtgg ttgacggcca
     1741 gcagcgacta accacgcttt acatcctgct gacctatctt ggcgacatgg cgaagatgct
     1801 gggcaagtgt cgctttcaac ttcgcttcga gactcgcggc gagtccaatg aaccctttct
     1861 cgcccatatc gatccgactc gtgccggcga gaatgtggat tttttccata tctgcgaagc
     1921 ctatcgtgcc attgagcaat ggttttccgg acgtgacgga atgcataagc tcaaactgct
     1981 gcagcatgtg ctcaacgacg acgaggctgg ccgcaacgtg aaggtcatct ggttcgagct
     2041 ggcagcaaag gacgacccag tggcggcctt tactcgcctt aatgttggga agattccgct
     2101 gagcaacgat gagttgatcc gcgctctgtt cctgcgtaag gccgctgctg cggacgccgg
     2161 tcctgggcag gcgctgcgta tcgcctacga atgggatcag ctggagaagg cgctacagcg
     2221 tgatgccttc tggtacttcc tgagcaacgc accacctctg ggaagcaacc gcataggtat
     2281 gttgttcgat ctggtcgcca cctcaggtat gagcgcgctg ctgaaacagg acgcctacgg
     2341 cgttttccat gtattcagcg aacgccttaa agcctcctca gccacgactg aatggctcgc
     2401 ggtaaagcag actttcatgg ccttggatga gtggtacgag gatcgggaat tgtttcacgt
     2461 ggtcggtttc ctgatccagc aaggggaaac catcaacgcg ctgctggagg cggccgtgac
     2521 acgtcgtaag gccgagttcg cgggagagtt gcgccgcagg gcctttgctt tggcgattgg
     2581 cgccgatctg gcggcactgg acgacggcca attaacggca cgtatccgcg agcgttgcga
     2641 gggcgtcagc tacgcccgcg atccgggggc gattcgcacc ttgttgttat tgtttaatct
     2701 ggcaacgctg ctggaaaatc cacgctcgaa cctgcgcttc cagttcgaca gcttcaagcg
     2761 cgaaaactgg gatatcgagc atgtccgtgc tgtggcagca cgccgcccgg aacgtcataa
     2821 cgagcggttg gagtggctag gccactgcca ggggtatctg cagacccgag aggacgggca
     2881 ggcgctcgcg caagcaatcg aagacttcgt tgcgctcccg cagcgcagcg cggtggacgc
     2941 tttgttcgac ccgctctacg aggacgtgct gatctatttt cacgagagcg atgaagggga
     3001 ggagaacggc ctgtccaatc tgacgctgct cgaccgcagc accaatcgca gctacaagaa
     3061 tgcagtgttc gccttgaagc gtcagcagtt gttggaactg gatcagtccg gcattttcgt
     3121 gccactgtgc actcgcaatg tgttcctcaa gtgctacagc ccgcaggtag ataacgcgat
     3181 gttttggagc caagaggatg ctaatgcgta cggtgatgta atcgtcgaga ccttgaggcg
     3241 cttcttctgt tcaggtcggg aggtgcaggt atgagcaata acggtgccca gttgatcttc
     3301 agtgaattgc tcacccgcca tggtcgcatc caggtgcccc tgattcagcg ggactatgct
     3361 cagggccggg ctgatcagca agaggtgcgc gaggagtttc ttgccgcgct gcacgaggct
     3421 ttgcgcctgc ctccggaggc ggagagccta cctctgaatc tggatttcat ctacggcagt
     3481 gttgaggggc aggtagcttc atgcttccag ccacttgatg gccaacagcg gctaaccaca
     3541 ttgtttttgt tgcattggta tttggcttgg cgcgatggac gtggtgatga atttcgcgcg
     3601 ctgtgcactg acgaagcggg ctcgcgcttt agctaccatg tacggcccag cagcagcgag
     3661 ttcttcgatg cgctggtggc tttccagccc cgacgatccc ccgatgatgt aactgcggtg
     3721 agtgtgctaa taatcgacca gccttggtat tttcggaact ggcggctcga tccgacgatt
     3781 cagtcggcgt tggtcatgct cgacgatctt cataagtact tccgaagtga gcagggcctg
     3841 tatgccagat tgatcgatac tgagcgcccg gcaatcacgt ttcaattgct agaccttaag
     3901 cacttcggac tttcggacga cctctatatc aagatgaatg cgcgcggcaa gccgcttacc
     3961 ccatttgaaa ctttcaaggc gcgctacgag caaacgctga aggatctatt cccgaaagag
     4021 cagcgcgtgc tcgacgggca gtatgtctca atggcagaat ttttcgcgcg ccgcatggat
     4081 actagttggg cagatttctt ctggccccat cgcgatccaa atagcaatgt gttcgatgct
     4141 gcggtgatga atgtctttcg ggcggtcatt ttgataaccc ggtcaccgca ggccgatggt
     4201 ttcggggagg acgttaacct cctgcgtaat cgcttccgaa agaacagcta tgcgttcttc
     4261 catcagcggg attggctcga ccgacctttc tctgagatgc tggcggtgtt gctggaagct
     4321 tggagcgcgg ctggcagtga cataggcatg cagttgccgg acggtcgcta tttcaacgaa
     4381 aaggctgttt tcgacaagct gttatcagac cccactacgc ttggctatga ggacatcgtt
     4441 caactggcgg cctatggaca gtaccttaag acgcacggag acaaattcga atcgacggat
     4501 tttcaggcat ggatgcgggt cgttttcaac ctgtccgtga atacggaata caaccgcccc
     4561 gcggacctgc agcgcagcct ggccgcactc aatacgttgg cgccgcacat gacctcgata
     4621 ctggattatc tggcccgggc cgattcggag atcactggct ttaaccagca gcaggtggcc
     4681 gaggagaagc tcaaagcgaa actgctaatt gccgacaaca cctggcgagt cttgatcgaa
     4741 caggcggaag ggcacggcta ttttcgcgga cagatcggat tcctgctggc gttttccgga
     4801 gtgagcgtac atccggcagc cgagggggag gagccgtgga ctcatgagac gaccctgagg
     4861 ctgcagcatg agttcaaagg tgtgctgcag caggcgtccg ccatgttcgg cgacagcggc
     4921 ctaaacagcc tgccagacta tcgttgggaa cgcgcattgc tcgcactcgg caattacctg
     4981 ctggcggccc gccgcaacga ttcgtttctg gtcaatgcac aagcggaacc tgcaagttgg
     5041 aaacgcttgc tcaggggcgc gtggtcaggg tcgccgcagg cggcagtgct gtgctccctt
     5101 tggaaacgcc tacagggcac agcgaatctc gaggcgcaac tcgatgccgt gattgcagaa
     5161 gcttctggga tcgaggactg gcgcgctgca cttattgcca ctcctgcggc cattggttac
     5221 tgtcaccagc gaatgattcg tcatgcggag aatgccagga catatctcct aaagaagttg
     5281 cagatgaacg gtgctcatgc cgagctgttt acctattgtc tttacgctca gacgcttcag
     5341 tctgccgatc gggaaggccg actacaggtg ctgacgccct attacgagga aacaaatact
     5401 gctgaagagg agccacacgt acgactccga ggtcaccatg gagatcggct gctgattttc
     5461 gttatcgagt tcgattacgt gcaatatgag atcaaactgc tggaaccaca tcccgaattc
     5521 ctgcctgatc ccctgttagc gttgtttcag aaaattggct tcgccctaac cggaacgcat
     5581 cttgtgaaac attctacaat tgataacata gagagcgaca ttttcgagct ggatcgacat
     5641 ctgcaaatta tgcgacgtaa cgtttaacgc atgaagataa tattgcacac tgcggaaacc
     5701 aaataaatga aacgtgattg gtctagaaaa actgtatgaa tgttatttgg tccgtggctg
     5761 caaacggttc acggttgggc cattcgctca tgcgaaatta aacgccctta cgggcggagt
     5821 caaataccgt aggcctagat ccgacaggaa gcttgagggt cacatagctg ccataggata
     5881 tgaagaagcc atcaggccgc aacgggtcgg ctgcactcga ttgcgcattc cgacgatcgt
     5941 gaccgctcat accgacgaac gtgaccggtg ttctgtgcgc gccagcgtgg tgttcagagt
     6001 ttaaccgcgt cggtcacgat cacggtcatt tcagggcatg gcataggctt atccacaggc
     6061 ggc
//
LOCUS       DAOQEW010000213         6048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_4988_length_6048_cov_7.436676, whole genome shotgun sequence.
ACCESSION   DAOQEW010000213 DAOQEW010000000
VERSION     DAOQEW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_6048_cov_7.436676"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..413)
                     /gene="gcvH"
                     /locus_tag="PLS35_16785"
     CDS             complement(27..413)
                     /gene="gcvH"
                     /locus_tag="PLS35_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNI20379.1"
                     /translation="MIPSNLRYHQEHEWVRAEGQQATVGISHFAQDALGDIVFIDLPK
                     VGAKVTAGQQIGEVESTKTTSTIYTPVSGTIAKINTELKDHPEAVNADPYGKGWMAVI
                     DLTTPGEVDKLMTAAQYEEFLSKQKH"
     gene            complement(555..2075)
                     /locus_tag="PLS35_16790"
     CDS             complement(555..2075)
                     /locus_tag="PLS35_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20380.1"
                     /translation="MQTRRPSSGRRASLPLVILLMALGSGCAGPPAVVPVAQPIENPV
                     VEAETRLWYQASSAFSDGRYSAATHLYERYLTTYPKSRRANEAHWELAQSYEQMGEAA
                     GALKEYRILAGPEGAPLSTQSAYAERALHRIEALRNQSSLPGARSGHTALYVSFSNLP
                     PMAQVETWIQRLSAQGVSAILMDASLESSFTRPPAPTGPPPSSPLPKLPAGALFSTSQ
                     GPVMTDWFSVMVPQAHELGISVFAVIDLFHAPLSDHRQESRTLLYDPTRRKVHSWTQF
                     DLLAPPVQQEVAQLLADLLRSGVDGMVIRARAENSFAYEVSDGVLRQFEAQVRQPASE
                     VAQALRERMSVRQDAGALASDKTLWRWVGWKSRQELDVLARLKKYLQKAVPRLRMVLE
                     IHPEALSNPTSALVNYGEDAAEARRRGFDLLLGGPSREASDVRTFGGSFKGWSRDVIQ
                     SEKGEPQTLPQGWVLLRTPTEHGGGMFMGLAQQADELRTPDIRHLVFVPDAPPAIP"
     gene            complement(2107..2526)
                     /gene="ndk"
                     /locus_tag="PLS35_16795"
     CDS             complement(2107..2526)
                     /gene="ndk"
                     /locus_tag="PLS35_16795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNI20381.1"
                     /translation="MSERTLAIIKPDAVKKDAIGDIINRYEKAGLKPVAMRLMHLSKT
                     TAQGFYAVHKARPFFDSLCTFMSSGPCVVLVLQGDNAVKKNRELMGATDPAKAENGTI
                     RAAHGANIEFNAVHGSDAPETAKFEIGYFFPEMELVG"
     gene            complement(2681..3745)
                     /gene="mtnA"
                     /locus_tag="PLS35_16800"
     CDS             complement(2681..3745)
                     /gene="mtnA"
                     /locus_tag="PLS35_16800"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNI20382.1"
                     /translation="MVPTVEWKEGAVRLLDQSRLPSQVEFLDCRDYRSVAQAIRELKV
                     RGAPAIGVTAAMGVALGAQSVDADEYSSFVKQVDAICDHLAASRPTAVNLFWAIARMK
                     QKLASLKGMPIAEIKRELIRESQSILDEDIALCKAMGRHGATVIQNGQTILTHCNAGA
                     LATAGYGTALGVIRAAWEQGKQIRVIADETRPVLQGARLTAWELMQDKIPVTLITDNM
                     AGSLMRQGNIQVCVVGADRIAANGDVANKIGTYSVAVLARAHGIPFYVAAPYSTIDLA
                     TKTGAEIPIEERNPLEVTSIHGSHPVAPSGVAVYNPAFDVTPAELITGIITERGIFKP
                     GELAGAFGTSRPPIKSGNVS"
     gene            complement(3871..4938)
                     /locus_tag="PLS35_16805"
     CDS             complement(3871..4938)
                     /locus_tag="PLS35_16805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNI20383.1"
                     /translation="MTLIPHSKPSLGQEELRAVTEVLRSGQITAGPMVAQFERGIAAY
                     IGLHGGVAVSSGTVALELALRALGVGHGDNVILPSYVCSAPWLAVQRVGAQARLVDIN
                     PTTYNLDPQKVRKARTSRTRAVIVPHMFGLPADLTSLQSLDIPLIEDCAQTLGAMEQG
                     RAVGSVGTLTVCSFYATKLLCTGEGGMVLSNDEALLERVRALREYDKAPSLNSAAGNC
                     KLTDLQAAIGVAQLNQLGEFLDKRSALAALYRDMLSPELFTMPVVPAGRTHVYFRFVV
                     RLMNGLQSADDFTDYLSRLAHRGLQCRKPVFRPLHRYLDLPDFPASDEADASAISLPI
                     HPSLTEEAIRQAAHILQEELH"
     gene            complement(5096..>6048)
                     /locus_tag="PLS35_16810"
     CDS             complement(5096..>6048)
                     /locus_tag="PLS35_16810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20384.1"
                     /translation="SPYVKDRALASVVRERLASGVYGAVHVEKLGLIASLPTQLAIPV
                     VLDIWSYGLPSPLGIFKSGGGTTGRPKPFVRRLKLGLYDRWCWPLTSCVTVVSEEDRI
                     RCEGAHPGQRVIVVPNGVDCQAIRPKSDHALTSPVLLFSGDMGAEPNVEAATVLATEV
                     FPAIRREFPKAELRLVGRNPDPRVARLAGPGIMVTGAVPDMQVHLRAATIYVAPHFTG
                     PATRTKLLEAMAAGLPIITTSLGTEGMKVQPGRDLLVADQPADMIESIQTLLASQPDR
                     ERFAQAARHMAEVWYDWSRCLWPLEPLYQNLLAPKPAVAC"
BASE COUNT         1277 a         1874 c         1729 g         1168 t
ORIGIN      
        1 attcaatggt gcaaggatgt tcggagttag tgcttctgct tgctcaaaaa ctcttcgtac
       61 tgagcagccg tcatgagctt gtccacttca cccggagtcg tgagatcaat gacagccatc
      121 caccccttgc catacggatc ggcattgacg gcctcgggat ggtctttgag ctccgtgttg
      181 atcttcgcaa tggtgccgct gacgggcgtg taaatggtgg acgtggtctt ggtcgactcc
      241 acctcgccga tctgctgacc ggcagtcacc ttggcaccga ccttcggaag atcgatgaac
      301 acgatatctc ccagcgcatc ctgtgcaaaa tggctgatcc cgaccgtcgc ctgttgacct
      361 tcggcgcgga cccattcgtg ttcctgatgg taacggagat tggacggtat catgactctc
      421 cccttgcatc ggtcgctgac acccctccca gtagcagtct cagtccactg gaaatgctcg
      481 gcgcgacctc gttgataaga agccccttca atattatttg tgccgccgga tcgtaaaagc
      541 tgccggaatc tttgtcaagg aatcgccggc ggtgcatccg gcacaaatac cagatggcgg
      601 atgtccggcg tccgcaattc gtccgcctgc tgggccagtc ccataaacat gccaccacca
      661 tgctcggttg gcgttcgcag caagacccag ccttgtggaa gagtttgcgg ctcgcccttc
      721 tcggattgaa tcacatcgcg actccagccc ttgaatgacc cgccgaacgt gcgtacatcc
      781 gatgcctcac gggacgggcc gcccaataac aaatcaaaac ctcggcggcg ggcctcggcg
      841 gcgtcctctc catagttcac cagggcggac gtcggattcg acaaggcttc aggatgaatc
      901 tccagcacca tgcgtaaccg cggcacggcc ttctgcaaat atttcttcag gcgcgccagc
      961 acatcgagct cctgccgtga tttccagccg acccaccgcc aaagcgtctt gtcggaggcg
     1021 agcgcccccg cgtcctgccg cacggacatc cgctcacgga gcgcctgcgc cacttccgac
     1081 gccggctgac gtacctgcgc ctcaaactgg cgcaacacgc cgtcactgac ctcataggca
     1141 aagctgttct ccgcgcgcgc gcgaatgacc atgccgtcaa ccccgctcct cagcaagtcg
     1201 gccaacaact gcgccacttc ctgctgaacc ggcggagcga gaagatcgaa ctgcgtccag
     1261 gagtgcacct ttcgccgcgt cgggtcatac agcagcgtcc gggattcctg ccggtgatcg
     1321 gagagcggcg cgtgaaaaag gtcaatcacg gcaaagaccg agatgcctag ttcatgcgcc
     1381 tgcggcacca tcacactgaa ccaatccgtc atgacaggcc cctgcgaggt cgaaaacaac
     1441 gcgcctgcgg gcaacttcgg caaggggctg ctgggaggag gaccggtcgg tgcaggcggc
     1501 ctggtaaacg acgactccaa actggcatcc ataagaatgg cgctgacgcc ctgtgcgctc
     1561 agccgctgaa tccaggtttc cacttgagcc atcggaggca aattgctgaa cgatacgtag
     1621 agtgcggtat gaccagacct ggcgccgggc aggctcgact gattccgcaa ggcttcgata
     1681 cgatgcaggg ctcgctcagc ataggcgctc tgcgtcgaaa gcggagcacc ctccggccct
     1741 gccaatatcc gatactcttt cagcgcgccc gccgcctccc ccatctgttc atacgactgc
     1801 gccagttccc agtgggcttc attcgcgcgc cgcgacttcg gataggtggt cagataccgt
     1861 tcgtagagat gcgtcgccgc ggaatagcgg ccgtcagaaa aggcactcga agcttggtac
     1921 cagagcctgg tctcggcctc gaccaccgga ttttcgatgg gttgagcaac cggaacaact
     1981 gcgggcggcc cggcgcaacc gctgccgagc gccatcagca ggatcaccag gggaagggat
     2041 gctcgacggc cggaagacgg ccgtcgagtc tgcatgacgt gggaaacgct tgcgctcaat
     2101 gtgcgcctac ccgaccaact ccatttcagg aaagaaatac ccgatttcaa acttcgccgt
     2161 ctccggcgca tcggacccat gcaccgcatt gaattcgatg ttcgcgccat gggccgcccg
     2221 gatcgtgcca ttctccgcct ttgcgggatc cgtcgccccc atcagttcgc ggttcttttt
     2281 caccgcgttg tcgccttgga gaaccaatac cacacagggc cccgaggaca tgaacgtaca
     2341 caggctatca aaaaatgggc gtgccttgtg aacggcataa aacccttgcg ccgtggtttt
     2401 ggacaggtgc atcagacgca tagcgaccgg cttcaacccc gccttttcat atcgattaat
     2461 aatgtcgccg atggcatcct ttttcacggc gtccggcttg atgatggcaa gggttcgttc
     2521 actcatggat cgtcagctcc ttcagctaaa aatatcgcaa cggcctactc tcgcggcatt
     2581 ataaagaagc gacggaagtg ctggcaagat aaagggctct gagattcttc ggcactcgag
     2641 gatgagtaat aatgtgccac acctacccga gaagccagta tcaggaaacg ttgccggact
     2701 tgatcggagg gcgtgacgtc ccgaatgcac cggcgagttc ccccggcttg aaaatgcccc
     2761 gttctgtgat gatgccggtg atcaattcgg ccggagtcac gtcaaacgcg ggattataga
     2821 cagcgacacc ggacggcgcg acaggatggc tgccgtggat agacgtcact tcgagggggt
     2881 ttcgttcttc gatgggaatc tcggcaccgg tcttggtggc gagatcaatc gtgctgtacg
     2941 gtgcagccac ataaaaagga atcccatgcg cccgggccag cactgcaacc gagtaggtac
     3001 cgatcttgtt ggcgacatca ccattggccg caatccgatc cgcgccgacg acacacacct
     3061 gaatattccc ctgccgcatg aggctgccgg ccatattgtc cgtgatcagc gtcaccggaa
     3121 tcttgtcctg catgagttcc caggccgtca agcgcgcgcc ctgcaagacc ggtcgggttt
     3181 cgtcggcaat cacacgaatc tgttttccct gttcccacgc agccctgatg acacccagcg
     3241 cggtgccgta ccccgccgtc gccaatgcgc cggcattgca gtgggtcagg atcgtctggc
     3301 cgttttgaat cacggtcgcc ccatgacggc ccatggcctt gcagagcgcg atgtcctcgt
     3361 cgagaatgct ttgcgattca cggatcagct cacgcttgat ctctgcgatc ggcataccct
     3421 tgagtgatgc cagcttttgt ttcatgcggg cgatggccca gaacaaattc accgccgtcg
     3481 ggcgcgaggc cgccaggtga tcacaaatcg cgtcgacctg tttgacgaac gaggaatact
     3541 cgtcggcgtc cacactctgc gcaccgaggg ccacgcccat cgcagccgtc acaccgatcg
     3601 ccggagcgcc gcggactttc aactctcgaa tggcctgcgc gactgagcga tagtcgcggc
     3661 agtcaagaaa ttcgacctgc gatggaagcc gactctgatc caaaaggcga accgctccct
     3721 ctttccactc gaccgtgggc accatgccga ttgtctccgg gttcagctgg ggaagaatgt
     3781 gacgccgtca catcacgttg aacattcgct cacatcatgc cgatcactca ggcaagggtc
     3841 aagggagaac gattcggcgg gacgcctgcg tcagtgcagc tcttcctgca ggatgtgcgc
     3901 agcctgccgg atcgcttctt ccgtcagtga gggatgaatc ggcagcgaaa tggccgacgc
     3961 atcggcctca tcgctggcgg gaaagtccgg caagtcgaga tagcgatgca ggggacgaaa
     4021 caccggcttg cggcattgca atccgcggtg ggccaggcgg gaaagatagt ccgtaaagtc
     4081 gtcggcagac tgtaatccgt tcatcagtcg gaccacgaac cggaaataga cgtgcgtccg
     4141 accggcaggc acgacaggca ttgtaaacaa ctccggcgac agcatgtcac gatacaaggc
     4201 ggccaaggcc gaccgcttgt cgagaaactc tcccaactga ttcagttgcg cgacgccgat
     4261 cgccgcttgc agatccgtca atttgcaatt gcccgccgcc gaattcaggg atggcgcctt
     4321 atcatattcg cggagcgcgc gaactcgctc gagcaaagct tcatcgttcg acaagaccat
     4381 cccgccttca ccggtacaga gcaacttcgt cgcataaaac gaacaaaccg tcagggttcc
     4441 aacgctgccg accgcgcgtc cctgctccat ggcaccgagt gtttgtgcgc aatcttcgat
     4501 caacgggatg tccaatgact gcagactggt caaatccgcc ggcaggccga acatatgggg
     4561 aacgattacc gcgcgcgtac ggctggtgcg ggctttgcgc actttctgag gatcgagatt
     4621 ataggtagtc ggattgatat cgacgagtct ggcttgggcg ccgacccgtt gcacagccag
     4681 ccagggtgcc gagcacacat aactggggag gatgacattg tcgccatggc cgacgccgag
     4741 cgcgcgcaaa gccaattcca acgccacggt gccggaactg acagccacgc cgccatgcag
     4801 gccgatgtag gcggcgatcc ctcgttcaaa ttgcgcgacc atgggcccgg cagtgatctg
     4861 gcccgaccga agcacctcgg tcactgctcg tagttcttct tgtcccagcg agggcttgga
     4921 gtgcgggatc aacgtcatgg tgtcagtggg aggctttgct caatcgtcat agggcctacg
     4981 acgtgcacgg gcacaaaatt ctacgtggac ggtaggcaga acacaacaga ttcctgaagc
     5041 gggggttgca ctgagcgcga tgacgtggca ggcatagctg gttgcgacca caccctcaac
     5101 aggctacggc gggcttcggg gcgagcagat tttgatagag cggctccagc ggccagagac
     5161 agcggctcca gtcataccag acctctgcca tgtggcgggc agcctgggca aaacgttcac
     5221 gatcgggctg actcgcgagc aacgtctgga tggattcgat catatcggcc ggttgatcgg
     5281 ccacgagcag atcacggccc ggctgcactt tcatcccttc agtcccgagt gaagtggtga
     5341 tgatcggcaa tcccgctgcc atggcttcca acaatttcgt gcgcgtcgcg gggccggtga
     5401 aatgcggcgc aacatagatg gtcgcggccc tgagatggac ctgcatgtcg ggaactgccc
     5461 cggtgaccat gatccctgga ccggccaatc gagctacccg cggatccggg tttctcccca
     5521 ccagccggag ttccgccttg ggaaattccc ggcgaatcgc aggaaagact tccgtggcga
     5581 ggaccgtagc ggcctccaca ttcggctcag cgcccatgtc gcctgagaag agcaacacgg
     5641 gtgacgtcag ggcatgatcg gatttcggac gaatcgcctg acaatcgaca ccgttgggca
     5701 cgacgatgac acgctggcct gggtgtgcgc cctcacaacg tatccggtct tcctccgaaa
     5761 caaccgtgac acaagaggtc aacggccagc accatcggtc gtacaacccc aatttgagcc
     5821 gacgcacaaa tggcttcggc cgaccggtcg ttccaccgcc gctcttgaat atccctagcg
     5881 ggcttggcag accgtatgac caaatatcca acaccaccgg gattgccaat tgcgtcggca
     5941 acgatgcaat cagtccaagc ttttcgacgt ggactgcgcc atagactcca ctggccaacc
     6001 gctcgcgcac gacagaagcc aaggcgcgat ctttcacgta ggggctga
//
LOCUS       DAOQEW010000214         5961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5110_length_5961_cov_9.443278, whole genome shotgun sequence.
ACCESSION   DAOQEW010000214 DAOQEW010000000
VERSION     DAOQEW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5110_length_5961_cov_9.443278"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLS35_16815"
     CDS             <1..365
                     /locus_tag="PLS35_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20385.1"
                     /translation="DWIAEQLDRGIDVLITNPELVKTGLDLLEFPTIVFMQSGYNVYS
                     LQQAARRSWRIGQKLSVRVMYLGYAGSSQMTCLELMAKKIMVSQSTSGDVPESGLDVL
                     NQDGDSVEVALARQLVTA"
     gene            510..1283
                     /locus_tag="PLS35_16820"
     CDS             510..1283
                     /locus_tag="PLS35_16820"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HNI20386.1"
                     /translation="MVIEAAYADGTGAANALHMSQAQWEELQRAYCVGDLLMPCCNAP
                     AIPKVSANGYPFFAHLGGACSTSEESQWHLAAKILVRSVLEDLGCRASVEMPGSGDAG
                     RWQADVWGERNGVRLAVEIQRSYQSLRDYRKRQERYREAGVKSLWLLRQERYSTLTKS
                     MGKERLRTEFGGKFPSAGHFGPCLSDLPVAMLELDPAPTVKGAGFFNATLPNILEAVL
                     SERFLCINGLWCIDNLDSMNNAARLSRERFAANRLAAKM"
     gene            complement(1350..3167)
                     /locus_tag="PLS35_16825"
     CDS             complement(1350..3167)
                     /locus_tag="PLS35_16825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI20387.1"
                     /translation="MTPSRSVSTAYLAQAAELAGNPGQLAAYNSQGHCVVLAGPGSGK
                     TKTLVLKLARILAEDVEAPRGVACITYSQECARELARRIESLGLQQAPNLFIGTVHGF
                     CLRHLLMPYGRLAGLPISFPLSVATQRVSDRLLKQTGDALFGQNHPYKVIDLGRHRRS
                     VLNRNSVAWRSEEELAAWAETYEAALRDEGLIDYDDMVVFGQRLIAEHDWVLPLVQAK
                     FPVLAVDEYQDLGVALHRIVKRLAFDGGVRLFAVGDADQSIYGFTGADGALLMELAAR
                     RDIEPVQLQLNYRSGAGIVTASEMALGEARGYQASDPARQTTIEFVLRPGGTADQAAH
                     AVAQIIPAALASKPGRTLGDIAILYKDYRAGDIVAKAVATGGLDYIRVDTAAPYRKVA
                     LTSWVEDCAAWCAGGWRVGRPQLRGLLDRYRAFHRASLDDSQAKREEQELTALLWELR
                     ADQQPAREFVASLRNGVVDHLLAAELALADQKEQLDRMTAALAEGGALASLDITSLGG
                     RDGSPDHLNLLTLHSAKGCEYDVVIMVGLDLGNLPWRNETPEKLRESRRLFYVGLTRA
                     RDEVHMLYSGFVDGRYGPMRFGRSPFLDELEARMRAAGI"
     gene            complement(3151..4938)
                     /locus_tag="PLS35_16830"
     CDS             complement(3151..4938)
                     /locus_tag="PLS35_16830"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI20388.1"
                     /translation="MRVSRVRLINFANFSDVDVETGESIVIVGENKVGKSNFIRGLQL
                     ILDPGLSERDRQLGLEHFWDGLGEDKVGATIEVSVDLTDFTNDPRLMAHLNDCVIDPG
                     PPMVARLTYRFQPKAGLGRAPESLKDYEYVIFGGDDPDMRIGGALRRMLPIDVQVALR
                     DAEKDLASWRNSPLRPLIEDLAASLDDDAREEIQDQVDQAQRELAGHEEVVATAERIS
                     ERLIAIAGGQHAVPVSLGLAPTRVDALLRSLRLLIDNGVRGVGDASLGTANLIFLALK
                     SLELDRLVSDGERDHTFFVVEEPEAHLHPHVQRLIYRYFLGTGAEDGEEQPPLTTILT
                     THSPHIASVAPIRSIVLLRHDTAEGKTVAVSTANAPFTQRDEEDLQRYIDVTRGEIFF
                     SRGVILVEGDAERFIVPAFAEVLDIPLDMLGITVCSVGGTNFTPYVKLLGPEGLNIPH
                     VILTDRDPTNGNHPLVRRRLINVLDVIEGGVDHENLDADEVIELAEQYGYFVNENTLE
                     PELFAGGLAQDMQEVIREELPRLRRETLNALQQWVDDPDQVDEDWLLRLIERIGKGRF
                     AQALAPSVSQDVCPAYIRSALEHIRDAIA"
     gene            5140..5760
                     /locus_tag="PLS35_16835"
     CDS             5140..5760
                     /locus_tag="PLS35_16835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HNI20389.1"
                     /translation="MFQDAELMCPSPPWFHHPERRLLAGVLGLLWSVLAGGCATTTAP
                     PAPDAIEEVSAAPEPEAPEYIPVVRYGRYTLVELAPRAAQRDLLLQTIDVSMPEDARA
                     TVGDGLRHVLKRSGYSLCQTAHAVIELYALPLPAAHLHLGPMTLRDALLTLAGPAWEL
                     HADDRARQICFERPADSAVIEPASEPPAAEAVQTFPLEPSVSGGQP"
     gene            5757..>5961
                     /locus_tag="PLS35_16840"
     CDS             5757..>5961
                     /locus_tag="PLS35_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20390.1"
                     /translation="MSSPQSARRPGAVVVVQSLLWLWLVFLSVLVALGYQALSDQADQ
                     ERLDSRLQRLEAQATGLAETIEAI"
BASE COUNT         1209 a         1825 c         1704 g         1223 t
ORIGIN      
        1 aggactggat cgccgagcag ttggaccgtg gcatcgacgt gctcatcacc aatccggagc
       61 ttgtcaagac gggattggac ctgttggagt ttccgacgat cgtgttcatg cagtcgggct
      121 acaacgtgta ttcgctccag caggcggcac gccgctcttg gcgtatcggg cagaagctgt
      181 ccgtgcgcgt gatgtacctg ggctacgccg gctcctcgca gatgacctgc ctggagctga
      241 tggccaagaa gatcatggtc tcgcagtcca cttcgggcga tgtacccgaa tcggggctcg
      301 atgtcctgaa ccaggatggt gattccgtgg aggtcgcatt ggcccggcaa ttggtaactg
      361 cgtgatctgc tgtctgccgg catcctttcg ggggtgccgg catttttcta ggaaattgct
      421 gctcggagga ttcgatcacc gccccgctcc cagcgcgggg aaggctaaac tgattagaca
      481 ataccgagat tcaaggaggc actgtggcta tggtgatcga agcagcatat gcagatggta
      541 ctggtgcagc caacgcactg catatgtctc aggcgcagtg ggaagagttg caacgggcgt
      601 actgcgtcgg tgatctgctg atgccttgct gcaacgctcc agcgattccc aaggttagtg
      661 ctaatgggta ccccttcttt gcgcatctag gaggtgcgtg cagtacttcc gaagaaagcc
      721 aatggcacct ggcggccaag atacttgtgc gcagcgtgct tgaagatctt ggatgccgtg
      781 cctcggttga aatgccgggt tcgggtgatg caggtcgctg gcaggctgat gtctggggtg
      841 agcgcaatgg ggttaggttg gctgtcgaga tccaaagatc gtatcagtcg ctccgtgact
      901 accgtaaacg ccaagagcgg taccgcgagg cgggcgtcaa gtctctttgg ttgctgcggc
      961 aggagcgata tagcacactc accaagagca tgggaaagga gcgtctgcgc accgagtttg
     1021 ggggcaagtt cccttcggcc ggccacttcg gcccgtgcct ttctgacctg ccggtcgcga
     1081 tgcttgagct agatccggcc ccaacggtca agggtgccgg attcttcaac gcaacccttc
     1141 caaacatact tgaagcagtg cttagtgagc gctttctttg catcaacggt ctatggtgta
     1201 tcgataacct agactcgatg aataacgccg ccagactctc gcgcgagcgt tttgcagcca
     1261 atcgcttggc tgcaaagatg tagctcgact gtggtgttgc tcgacatgcg ccgacgcagc
     1321 accgacactc cagattccct ggttcacact caaatgccag cggcacgcat tcgcgcctcc
     1381 agctcatcaa ggaatggcga gcgcccaaag cgcatagggc catagcggcc atcaacgaag
     1441 cccgaataca gcatgtggac ctcgtcgcga gcacgtgtga gtcccacata gaagagccgg
     1501 cgactttcgc gcaacttctc tggagtttca ttgcgccacg gcagatttcc gaggtcgagt
     1561 ccaaccatga tgacgacgtc gtactcacag ccctttgcgg aatgcagtgt cagcagattc
     1621 aggtgatcgg gcgaaccgtc ccgaccgccc aaactagtga tgtctagcga agccagtgca
     1681 ccgccttcgg cgagcgctgc cgtcatgcga tccagttgct ctttctgatc agcgagcgcc
     1741 aattcagccg ccagcagatg atccactaca ccgttgcgca acgaagcaac aaattctcgc
     1801 gcaggttgct ggtcagcgcg cagctcccat agcaaggcgg ttagctcttg ctcttcgcgc
     1861 ttggcctgtg aatcgtccaa gctcgcccga tgaaatgcgc gatagcggtc gagtagtcca
     1921 cgcaattgtg ggcgtccaac acgccagcca cccgcgcacc acgccgcaca atcttctacc
     1981 caacttgtta gggcaacctt gcggtaaggt gctgcggtat ccacacggat gtaatcgaga
     2041 ccgccggttg ccacagcttt agccacgata tcgcctgcgc gatagtcttt gtataggatc
     2101 gcgatatcgc ccagtgttcg tcccggcttg gaggctaagg ccgccggaat gatctgcgcg
     2161 acggcgtgcg cagcctggtc cgccgtacca cccgggcgca ggacaaattc gatggtcgtt
     2221 tgtcgcgcag gatcgctcgc ttgatagcct cgggcttctc caagcgccat ctctgacgca
     2281 gtcacgatcc ctgcaccaga acggtaattt aattgaagct ggacaggctc aatgtctctg
     2341 cgagccgcca attccatgag caacgcgcca tcagcccccg taaatccata tatcgactga
     2401 tccgcatccc cgacagcgaa aagtcggaca cccccgtcga aggcaaggcg tttgacgatg
     2461 cgatgcagtg ctacacctaa atcctggtac tcatccaccg cgagcacggg gaattttgcc
     2521 tgaaccagag gtagtaccca gtcgtgctcg gcgatcaaac gttggccgaa gacaaccatg
     2581 tcatcgtagt caatcagccc ctcgtcgcgc agagcggcct cgtaggtctc ggcccaggca
     2641 gcgagttcct cctcgctacg ccaagcgacg ctatttcgat tgagcacgga acggcgatgc
     2701 cgtccgaggt caataacttt gtacggatgg ttctggccga aaagcgcatc tccagtttgc
     2761 ttcaacaacc gatcactgac tcgttgagtg gccaccgaaa gtgggaaaga tatgggcagg
     2821 ccggccaggc gtccatacgg catcaagaga tgacgcagac agaacccatg gaccgtacca
     2881 atgaaaagat taggtgcctg ctgaagtccc aagctttcaa ttcggcgagc gagttcgcga
     2941 gcgcactcct gactataagt gatgcacgca acgccacgag gggcctcgac gtcttcggct
     3001 aggatgcggg cgagctttag gacgagtgtc ttggtcttac cgctcccggg accggcgagt
     3061 accacacaat gtccttgaga gttgtaggct gccaactgac ccggattccc agccagttcg
     3121 gcagcctgag ccaagtaggc tgtgctgaca ctacgcgatg gcgtcacgga tatgctccag
     3181 agcggaacga atgtaagccg gacaaacgtc ctgtgacacc gacggtgcaa gtgcctgcgc
     3241 aaacctcccc tttccgatcc tctcgatcaa tctgagcagc cagtcttcat caacctgatc
     3301 aggatcgtcc acccactgct gcaaggcatt cagagtttct ctccttaatc gcggcagttc
     3361 ctcccgaatt acctcctgca tgtcctgcgc cagtccacct gcaaatagtt cgggctccaa
     3421 cgtgttctcg ttgacgaagt atccgtactg ctcggcaagc tcgatcactt catccgcatc
     3481 caagttttcg tgatcgacac cgccctcaat cacgtcaagg acattgataa gccgcctgcg
     3541 aaccaatgga tgattaccat tggttggatc ccggtccgtc aggatcacat ggggaatgtt
     3601 gagtccttca gggcccagta gcttcacata cggggtgaag ttagtccccc ccaccgagca
     3661 cacagtgatt ccaagcatat cgaggggaat atcgagtacc tcggcaaacg cgggaacgat
     3721 gaagcgctcg gcgtcgcctt cgaccagaat caccccccga gaaaagaaaa tctcgccgcg
     3781 agtgacgtca atgtaacgtt gaagatcctc ttcatcccgc tgcgtgaaag gtgcgtttgc
     3841 tgtcgagacc gcgacagtct tgccttccgc tgtgtcatgg cgcagcagaa caatagatct
     3901 gatcggcgca acgcttgcga tatgtggcga gtgggttgtg aggattgtcg taagcggtgg
     3961 ctgttcctca ccatcttccg cccccgtgcc aaggaagtaa cgatagatta gacgctgcac
     4021 gtgcgggtgc aggtgcgctt cgggctcctc gactacgaag aaggtgtggt cgcgctctcc
     4081 atcggaaacg aggcggtcca gttcgagact cttcagcgcc aggaagatca ggttcgcggt
     4141 tcccaaactg gcatcgccga ctccgcgtac gccgttgtcg atcagcagcc gcaggctgcg
     4201 cagcaacgcg tcgactctag ttggtgcaag tccgagcgat accggaacgg catgttgccc
     4261 cccagcgatg gcgatcagcc gttcgctgat ccgttctgct gtcgctacca cttcctcgtg
     4321 tccagccagc tctcgctgtg cttggtcgac ctgatcctga atctcctcac gggcgtcatc
     4381 atccaacgac gcggcaagat cctcaatgag cggcctcaat ggcgaattgc gccagctcgc
     4441 aaggtccttc tcagcgtcac gcagggctac ctgaacatct attggcaaca tccggcggag
     4501 tgcgccgccg atacgcatgt cgggatcgtc gccaccgaag atcacatact catagtcctt
     4561 caacgattcc ggagcgcgcc ccaggcccgc cttaggctgg aagcggtagg tgagtcgggc
     4621 caccatgggt gggccaggat caatcacgca atcgttgagg tgagccatca gtcggggatc
     4681 gtttgtgaag tccgttagat ccacagagac ctcaatggtc gcaccaacct tgtcttcgcc
     4741 aagtccgtcc cagaaatgtt caagccccag ttgtcgatcg cgctcagaca agcccggatc
     4801 aaggatcaac tgcaggccgc gaatgaaatt gctcttgcca accttgttct ctcccacgat
     4861 gacgatactt tcccccgtct cgacatcaac atccgagaaa ttggcgaaat tgatcaacct
     4921 gacccgagaa accctcattt acgcccccgt ccactggtgt tttaacaaat agtagcagtc
     4981 aagcggccgg cctccatccg ccgaccgcga ccgagctttt ccgttacggc cccctcaagg
     5041 ccgcttttgt tcgactagac cgctgccggt aaatcggacg cagcgtggtt cgcattgttt
     5101 gctgctgccc cgggtcttag cggcgatggt gagggctcga tgtttcagga cgccgagctt
     5161 atgtgcccct ctccaccttg gtttcaccat cccgaacgcc gcctgctggc tggagtcctc
     5221 ggcctgctct ggtcggtgct ggctggcggc tgcgctacga cgactgcgcc gcccgcacct
     5281 gatgccatcg aggaagtctc ggccgcgccc gaacccgagg cgcccgagta catccccgtc
     5341 gtgcgctacg gtcgctacac gctggtcgag ctggcaccca gagcggcgca gcgcgacctg
     5401 ctgttgcaga ccatcgacgt gtccatgccc gaagatgccc gtgccaccgt cggcgatggg
     5461 ctgcggcatg tgctcaagcg tagcggttac agcctgtgcc agacggcaca cgcggtaatc
     5521 gagctgtacg cgctgccgct gccggcggcg cacctgcacc tcggccccat gaccttgcgc
     5581 gatgcgctgc tcacgctggc tggcccggcc tgggaactgc acgcggatga ccgcgcacgg
     5641 caaatctgct tcgaacggcc cgccgacagc gcggtcatcg aacccgcatc cgagccaccc
     5701 gccgccgagg cggtgcagac gttcccgctg gagccttcgg tttcgggagg ccagccatga
     5761 gctccccaca gtctgcccgg cgcccgggcg ccgtcgtggt ggtgcagagc cttctgtggc
     5821 tctggttggt cttcctcagc gtcttggtgg ccctgggcta ccaggccctc agcgaccagg
     5881 ccgaccagga gcggctggat tcccgcctgc aacgcctgga agcgcaggca actggcttgg
     5941 ccgagacgat cgaggccatc c
//
LOCUS       DAOQEW010000215         5933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5156_length_5933_cov_6.949643, whole genome shotgun sequence.
ACCESSION   DAOQEW010000215 DAOQEW010000000
VERSION     DAOQEW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5156_length_5933_cov_6.949643"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..875
                     /locus_tag="PLS35_16845"
     CDS             291..875
                     /locus_tag="PLS35_16845"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNI20391.1"
                     /translation="MPPPKTTGSLWQEENGRAYLYEDLRAMRAGDIITILISEKHKGS
                     KSADTSAEKDSTVSNGLGGTGMGYLGLPGIRLGGEAKRGFGIDASAKNKFGGKGATNR
                     EDTLTGTISAIVTEVLPNGDLRIEGRREVTVNSERQIMTIAGIVRRVDVNTKNTVQSS
                     AIADAKIEYSGLGVVDDVQRPGWFVRILDWVYPF"
     gene            918..2228
                     /locus_tag="PLS35_16850"
     CDS             918..2228
                     /locus_tag="PLS35_16850"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNI20392.1"
                     /translation="MTKPRKQQRAMIMQCFRLMLSSGVLRTPSVDPELLARPWTPPPP
                     KPAPVSEPRQQMSPWFRRTAVGMLAFLTISFFSASLAHAVRVKDVATIEGVRENQLIG
                     YGLVVGLDRTGDQVIGGQFTIQAMMSMLNKMGINLVIDPIQLLTRNIASVMVTAKLPP
                     FVKPGMTLDAIVSSMANAKSLQGGTLLLTPLKAPNQQVFAVAQGPVSIGGFLGGTGGA
                     GGATVTKNHQAAGVVPAGAIVEKELVVDIDQWDTVSVLLRHPDFTTAIRTAEAIDGTF
                     GKGTAVPVNAGLVKTTLPSTFQGRVVEYIATMEGLDVSVDVAAKVVVNERTGTVVLGE
                     HVRLSTCAISHGNLTISVKNTLNVSQPPAPLIGSTQGQTTVTPDVQTDVAEEQSRLIV
                     VDQTVTLGEVVRALNAVGVTPRDLVAILSALRAAGALQANLEIV"
     gene            2246..2575
                     /locus_tag="PLS35_16855"
     CDS             2246..2575
                     /locus_tag="PLS35_16855"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNI20393.1"
                     /translation="MDIKNLLSGQLDLAQMNATQPAGLERVQGLEKAGQKSTEAMHKA
                     GQEFEAYFISYLIGKMRETVPKGLLDRKGEQVWYSFYDQEIAKLATQAGGIGLTAFVD
                     AYVEKNK"
     gene            2676..2981
                     /gene="flgM"
                     /locus_tag="PLS35_16860"
     CDS             2676..2981
                     /gene="flgM"
                     /locus_tag="PLS35_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNI20394.1"
                     /translation="MEISHNGRAADLAKILLGVQETDRTQNKKNASQTTPSQDRVQIS
                     ERAKELQRLRAAAEQPDHERDARVGQIQQSVEGGTYNVDGKKVADAIIRNVLTDAVL"
     gene            3057..3500
                     /locus_tag="PLS35_16865"
     CDS             3057..3500
                     /locus_tag="PLS35_16865"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNI20395.1"
                     /translation="MSTLSQTTPTESDMLVRGMLDKMLAFQSLLLEEQDAIRSLSFGQ
                     FTSVTTRKSQLLDEIRDFEQRRRQQTSSELSRQDARTTMEAKLLAAIGETDRLNRFNA
                     ALIGQSLEFLQGALKRWQRAPESAALYSSAGTAVPAGAGLVRAKG"
     gene            3512..4906
                     /gene="flgK"
                     /locus_tag="PLS35_16870"
     CDS             3512..4906
                     /gene="flgK"
                     /locus_tag="PLS35_16870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNI20396.1"
                     /translation="MSGLNGLFGVGSNALASFQRAMSVTGQNIANVSTPGYSRQEAVL
                     TETLPENGRPGQIGTGVQVSEIRRSVDSFVEQQLLNSNERIGQFGASQKALSQIQILF
                     NDANDQGIAAGLNEFFKAWQDVATNPADLTARTVLLTKADGLTKVLNQASSQLSTQRI
                     SLDGQIGSAINDINALASKIADLNAQIKLTEVGGQQANDLRDQRGRFLNDLAGLVDIS
                     SIEDGNGQVTVFVGVGQVLVTDHTAFKLTGVPDATNNGLLDVRYDGGTGPNTDITSSI
                     SGGRLKGLLDARDTTATGLQTSLDTLTAQLVSQVNTQHKLGYGLDGSTTQDFFTATGT
                     TAGTISMALTDRQKIAASSTAAGVPGNNVNALALANLQTASVAGLGNTTFQSYYGAMA
                     GSFGATLQGATRDLQGQEILHDQLLAHRAEVSGVSMDEELINMLKYQRAFQAASKLIT
                     TSDEMLQTILSLKR"
     gene            4937..5827
                     /gene="flgL"
                     /locus_tag="PLS35_16875"
     CDS             4937..5827
                     /gene="flgL"
                     /locus_tag="PLS35_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNI20397.1"
                     /translation="MRVADQQMYNTLLGNLQRSRLQMLTSQEQVSSQRRVSTPEDDPS
                     SYGQIVLDKSALSQTKQWIRNIDFGTSRVNAADQALGQVQTLITRVRELTIQASSDTT
                     SAEGRQSIAKEVRQLQRQLVQLGNTEVAGQAVFGGTKTNVTPYVITTGDTVTYQGNTE
                     TQSISVGENQTVQILVPGSSIFTGGTTNMFDSLRDLLTALESNNRAGIQTGIGNLDLA
                     TAQISDAQGTVGALANRLQVTHDALDTATLTISKAISDNQDADLATAISQLSLQQVAV
                     QAASQTFSKIFDSSLINYLR"
BASE COUNT         1344 a         1718 c         1759 g         1112 t
ORIGIN      
        1 gggcgatcgg gtgacgatcg aagcccggca ggggggactc tccattcaaa ccgtaggtgt
       61 gacgaagtcg cacggtgaac tgggtcaaac catcacggtg tccaatgttg attccggcaa
      121 ggatctacga gcgaccgtgg tcggtccggg ggtggtccgt gtcagcttct aacccatcat
      181 ccacaaggta tcagacgatc ggattcatcc tctgcgggct tgcgctctgc gccctgccag
      241 gctgttcgag ttcgtctgcc gcgaagcccg cgaaagcgga gttggccaaa atgccgccgc
      301 ccaagacgac cggctcgctg tggcaggagg agaacgggcg ggcctatctg tatgaagatc
      361 tgcgtgccat gcgtgccggg gacatcatca cgattctgat ctccgagaaa cacaagggat
      421 cgaagagcgc cgacaccagc gcggaaaagg attcgacggt gtccaatgga ctcggcggca
      481 caggcatggg gtatctcggc cttccgggta tccgactcgg tggagaagcc aagcggggat
      541 tcggcatcga cgccagcgcc aaaaataagt tcggcggcaa aggcgccacc aatcgcgaag
      601 acacgttgac cggcaccatc tcagccattg tgaccgaagt gctgcccaac ggcgatttgc
      661 gaatcgaagg acggcgcgaa gtcaccgtga actcggagcg gcagatcatg acgatcgccg
      721 gtatcgtgcg ccgtgtcgac gtcaacacca agaacaccgt gcaatcgagc gccattgccg
      781 atgcgaaaat cgaatattcc ggcctgggag tggtggacga tgtgcagcgg ccgggctggt
      841 tcgtcaggat tctggattgg gtctatccct tctaatgacc gagcagtagg accgacagga
      901 gcgagacagg agtcattatg acgaagccac gcaaacaaca acgggcgatg attatgcagt
      961 gttttcgtct catgctgtcg agcggcgtcc ttcggacgcc ctcggtggat ccagagcttc
     1021 tggcccgtcc atggacgccg ccgcctccca aaccggctcc cgtctcggaa cctcgacagc
     1081 agatgagtcc ctggtttcgc cgcaccgcgg tgggcatgct ggcgttcctg acgatctcct
     1141 tcttcagcgc ttcgctcgcc catgctgtgc gcgtcaaaga tgtcgccacg attgaaggcg
     1201 tacgggaaaa ccaattgatc ggatatggac tggtcgttgg tctggaccgt accggcgatc
     1261 aagtcatcgg cggtcagttc acgattcagg ccatgatgtc catgctgaac aagatgggca
     1321 tcaatctggt cattgatccg attcagttgc tcacgcgaaa catcgcgtca gtgatggtca
     1381 cggccaagct tccgccgttc gtcaaaccgg gcatgacgct tgatgcgatc gtatcttcca
     1441 tggcgaacgc caagagcctc caaggcggca cgttgctcct cacgccgttg aaggcgccga
     1501 accagcaagt gtttgcggtc gcgcagggtc ctgtgtcgat cggcggattt ttgggaggga
     1561 ccggcggcgc gggcggcgcc acagtcacca agaatcatca ggccgcaggt gtcgtgccgg
     1621 ccggggcgat cgtcgaaaag gaattggtcg tcgatatcga ccagtgggat accgtgtcgg
     1681 ttctcctgcg gcatcctgat ttcacgaccg ccattcgcac cgccgaagcc atcgacggga
     1741 catttggaaa aggaactgcc gtcccggtca atgcaggcct cgtcaagacc acgctgccgt
     1801 cgaccttcca ggggagggtg gtggaatata tcgcgacgat ggaaggattg gatgtgagtg
     1861 tggatgtggc ggctaaggtg gtggtcaatg aacgcaccgg tacggtggtg ctgggtgaac
     1921 atgtccgcct ctctacctgc gccatctcgc atggtaatct caccatttcc gtgaagaata
     1981 ccctcaacgt ctcgcaacct cctgcgccct tgatcggctc cacccaaggc cagaccacag
     2041 tcacgccgga tgtgcagacc gatgtggccg aagagcagtc ccggctgatc gtcgtggacc
     2101 agaccgttac ccttggagaa gtcgtgcggg ccctcaatgc cgtcggcgtc acaccacgtg
     2161 acctggtcgc gattctgtcg gcgctgcgtg cggccggagc acttcaagcg aatcttgaga
     2221 tcgtgtaaga aaggtctgga ccatcatgga catcaagaat ctgttgagcg gccagcttga
     2281 tctggcccag atgaatgcga ctcagcccgc aggcctcgag cgagtccaag gcctggagaa
     2341 ggcagggcag aaatccactg aagcgatgca taaggccgga caggagttcg aagcctactt
     2401 tatatcctat cttattggaa agatgaggga aacagtgcca aaaggcctgt tggacaggaa
     2461 gggggagcag gtctggtact ccttctacga ccaggagatt gccaagctgg cgacacaggc
     2521 gggaggcatt ggccttacgg ccttcgtgga tgcgtacgtc gaaaaaaaca aataaatcga
     2581 ccggttctcc taaagttcca ggccgacctc gccgatagag tactcgacta ggggaaagcc
     2641 tacggaacac cctgcaaagg ggaggagaag cagtcatgga gatctctcac aatggccggg
     2701 ccgcagatct cgcgaaaatt ctcttaggtg ttcaggagac tgaccggaca cagaacaaga
     2761 aaaacgcatc tcagaccacg ccgtcgcaag accgcgtaca gatttccgag cgggccaagg
     2821 aactccaacg tctgagggcc gcagcggaac aaccggatca cgaacgtgac gccagagtcg
     2881 gccagatcca gcagtcagtc gaagggggca cctataacgt cgacggcaaa aaagttgcgg
     2941 atgccatcat tcgtaacgtc ttgacggacg cggtcctgta acgagtcgct tcaccggctc
     3001 gacccttcgc tcgctgtcct cgtatgccgg aacgttactg tcgaggagga agcacggtgt
     3061 cgacgctctc gcaaactact cccactgaat cagacatgct cgtgcggggc atgctcgaca
     3121 aaatgctcgc gttccagtcc ttgctgctgg aggaacagga tgcgatccga tccctgtcct
     3181 tcggccaatt cacctccgtc accacgcgaa aatctcaact tctagatgag attcgtgatt
     3241 tcgagcaacg gcggcgtcag cagacctcgt ctgaattgtc tcgacaggac gcgcgcacca
     3301 cgatggaagc caagctgctt gcggcgatcg gcgagactga tcgcttgaat cgattcaatg
     3361 ccgcactcat cgggcagtcg cttgaatttt tgcaaggcgc cctgaaacgc tggcaaagag
     3421 ctccggagtc ggcggcgctg tattcgtcag ccggcacggc ggtcccggca ggcgccggtc
     3481 tggtgagagc caaaggataa cgagtggtcg tatgtcagga ctgaacggat tattcggagt
     3541 gggttccaat gcgctggcca gtttccagcg cgcgatgtcc gtcaccgggc aaaacatcgc
     3601 gaatgtcagc actcccggct attcgcgcca ggaggccgtc ctcaccgaga cccttccgga
     3661 aaacggacgc cccggccaga tcgggaccgg cgtgcaggtc tcggaaatcc gccgttccgt
     3721 cgacagcttt gttgaacagc aattgttgaa ctcaaatgag cggatagggc aattcggcgc
     3781 ctcacagaag gctctctcac aaattcagat tctgtttaac gatgcgaacg atcaaggcat
     3841 cgcggcaggg ctcaacgaat tcttcaaggc gtggcaggat gtcgcgacca atcccgccga
     3901 tctgacggcg cggacggtgc tcctgaccaa ggccgacggg ctgacgaaag tcctcaacca
     3961 ggcttcatcg cagctgtcga cccaacgaat ctccctcgac gggcaaatcg gaagtgccat
     4021 caatgacatc aacgcgctgg cgagtaagat tgcggacctg aatgcccaga tcaagttgac
     4081 cgaagtgggc gggcaacagg cgaatgacct gcgagaccaa cgtggccggt tcctgaatga
     4141 ccttgccggc ctggtggata tttcttcaat cgaagatgga aacgggcagg tcaccgtgtt
     4201 tgtcggggtc ggccaggtcc ttgtgacaga ccacacggcc ttcaagctca cgggcgttcc
     4261 cgatgccacc aacaacggat tgctcgacgt gcggtatgac ggcggaaccg gccccaacac
     4321 cgatatcacc tcatcgatca gtggcgggcg cctcaaaggt ctgctcgatg cgcgggatac
     4381 cacagcgacc ggcctgcaaa cctctctcga cacgctcacg gcgcaactcg tctcgcaagt
     4441 gaacacccag cataaattgg ggtatggctt ggacgggtcg acgacgcagg acttcttcac
     4501 ggcgacagga actacggcgg gcacgatcag catggcgctg acggatcgac agaagatcgc
     4561 cgcgtcctcg actgcggccg gcgtgccggg caataacgtc aacgccctcg ccctggcgaa
     4621 tttacagacg gcatcggtcg cgggcttggg aaacacgacg tttcaaagct actacggtgc
     4681 aatggccggg agcttcggcg cgaccctgca aggggccacg cgggacctgc aagggcagga
     4741 gattctgcac gatcaactgc tggcccatcg ggcggaagtg tccggcgtct cgatggacga
     4801 agagttaatc aatatgttga agtatcagcg ggcgtttcaa gccgcctcga agctgatcac
     4861 caccagcgac gaaatgctgc agacgatctt gtccttgaaa cggtaaccgt cattcagttc
     4921 acccggagca ggtattatga gagtcgccga ccaacagatg tacaacaccc tgctcggcaa
     4981 tctgcaacgc tcccggttac aaatgctgac ctctcaggaa caggtctcga gccagcgacg
     5041 ggtctcgact cccgaagacg atccgagctc ctatggccag atcgtcctcg acaagtccgc
     5101 gctgtcgcag accaagcaat ggattcgtaa tatcgatttc gggacttcgc gagtcaacgc
     5161 cgccgaccag gcactaggcc aggttcagac ccttatcacg cgggtacgcg aattgaccat
     5221 tcaggccagc agcgatacga cctccgccga aggccgtcag tcgatcgcca aagaagtccg
     5281 gcagctccaa cggcagctgg tgcaactggg caatacggaa gtggccgggc aggcggtatt
     5341 cggcgggacc aaaaccaatg tgactcccta cgtcattacc accggtgata cggtgaccta
     5401 tcagggcaac accgaaacgc agtccatctc ggtgggcgaa aaccagaccg tgcaaattct
     5461 tgttccgggt agcagcattt ttaccggcgg aaccacgaac atgttcgatt cgctccggga
     5521 tttgctcacg gcgctcgaaa gcaacaatcg tgcgggtatt caaacgggaa tcggcaatct
     5581 tgacctggcg acggcacaga tcagcgacgc gcaggggacg gtcggggcgt tggctaaccg
     5641 cctgcaggtc acccacgacg cgctggatac ggcgaccctg acgatttcca aggccatttc
     5701 cgacaatcag gatgccgacc tcgctacagc catcagccag ttgagcctcc agcaggttgc
     5761 ggtgcaggcc gccagccaga cgttcagtaa gatttttgac tcctcgttga tcaactatct
     5821 gcggtaacca acgctcaagt tcattctctc ctgtaccgat atacccaatg tacccgtatt
     5881 cacacgccaa ggaaggcttg tatgctggtc ttaacacgac gtcggggaga ggg
//
LOCUS       DAOQEW010000216         5928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5165_length_5928_cov_7.409671, whole genome shotgun sequence.
ACCESSION   DAOQEW010000216 DAOQEW010000000
VERSION     DAOQEW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5165_length_5928_cov_7.409671"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..496
                     /gene="greA"
                     /locus_tag="PLS35_16880"
     CDS             20..496
                     /gene="greA"
                     /locus_tag="PLS35_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNI20398.1"
                     /translation="MPTPITRKGYEALKAELDRLHKVERPRVIEAIAEARAHGDLSEN
                     AEYDAAKERQGFIEARLAELKGKLADCRIIDIAGRTSDTVVFGATVVLIEQEAQAKKQ
                     YTLVGQDEADLKFSRISVQSPVGRALIGKRVGDLVEVTTPAKVVEYEVMEIRFEEL"
     gene            576..1376
                     /locus_tag="PLS35_16885"
     CDS             576..1376
                     /locus_tag="PLS35_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNI20399.1"
                     /translation="MPVTIPLITLLTDFGDRDYFVASVKGVILNINPQAHIVDLSHHV
                     TPHDVADAAYLLKSCYRYFPDGTIHVAVVDPGVGTTRRPLLVSSSRYFFIGPDNGLFT
                     HIYQEETGVEVRHLENRQYRLDSEGATFDGRDLFAPAAAWLTKGQPIGSFGRLVPNYE
                     RLPISEPSWDKHVMAGEIAYIDRFGNLISNLTAYHIREVRGITKRADPYIRIGGVTID
                     CLVHSYGEGSADAPQALINSNGYVEVFLKEGRAADRLNVARGARIELS"
     gene            1718..2866
                     /locus_tag="PLS35_16890"
     CDS             1718..2866
                     /locus_tag="PLS35_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20400.1"
                     /translation="MKPLLQHLSVVLLCLTVMGCTRPDPPAPSAKPVQPSVVANGQPD
                     IQTMRIDASPSLPALTLSARVTYAEDGYSKISSPLPGPVLDVRVKLGQAVKAGDILMV
                     IDAADIARAYAAYVEEISELGLAERNYELTKDLYDAQAMSLKDLEHAENDLNRERAEF
                     KQAKELLLSLRVPAAELSKPLAQQQITSRFELRSPLTGTVVERNVTPGQIVGTASDQP
                     LFTVANLDRLQVVADVYEHDLSGIRVGALAAMTVEAYPAIEFPAKIAVIGDVVDPATR
                     TIKIRATVSNQDRRLKPEMFARLTIAGHTMRPKIVIPKQAVMERSGRQWIVVELAPGR
                     FEERPVTIDSVTDNQVTIREGVVTGERIVLSPSSINHLAASDTSAGGA"
     gene            complement(2895..4121)
                     /locus_tag="PLS35_16895"
     CDS             complement(2895..4121)
                     /locus_tag="PLS35_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20401.1"
                     /translation="MTTGLIIRVLCLLLFLPFSLWAEDQQPSELLLQRIQFSDTARES
                     GTAAIPFSIASPVMHTSFQATSFAPPTTSTRLKDLVPDSERPQTKGLGANSTWLKGQL
                     TAEGEVANNATNDAGLTSRIDQRDDGAKRMVRMALTGTNGPVRYGINYRSAGKAFFNS
                     PDQTVREMWAEWGLGIAKLRSSSGQTWNNVDLEPNRPRIQQDVSRIGIALVKPAWPEL
                     SLTYARSALASSYIPAGMTAQRSQSNSMEAALAYTGLSWNARLSSSYILTNDEMRGGA
                     QSTTFAQTLVASYRPLNTLTFAPTFSYRTETQSWSGAKIQTPMASVSLLYKQSQRLLV
                     SAMGGYTSTKSSDGLIETESIVGRGMFAFHLNPIYGHPTTLSLEASYTNTTNRTVAGV
                     DTEDLSGLVRILVAAL"
     gene            complement(4296..5678)
                     /gene="bioA"
                     /locus_tag="PLS35_16900"
     CDS             complement(4296..5678)
                     /gene="bioA"
                     /locus_tag="PLS35_16900"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNI20402.1"
                     /translation="MTPPSTRQPLAAWDHAYVWHPFTQMQEWEQESPLIIEKGKGSYL
                     IDTEGRKYLDGTASIWVNLHGHRHPRLDRALTAQLRQIAHSTFLGLTNPPAIRLAREL
                     IRLAPKGLRRVFYSDNGSTAVEVAIKMAVQYWQQARPSNGSKQSFAHLKMAYHGDTIG
                     AVSVGNIELFHGRFKPLLFPTHQVEPPYCYRCPLGRAYPSCDMACIDPLERLLKTRHR
                     ELAGVIVEPLVQAAAGMMVAPPGYLARVRELCTKYNVLLIVDEVATGFGRTGKMFACQ
                     HEGVTPDLMAISKGLTGGYMPLAATLTTDAIYRAFLGRYDEWKTFFHGHSYTGNPLGC
                     ALALANLEIFRRERTLAQVRKKSRLLARLLQPLAELPHVGDIRQCGFMVGIELVQDRQ
                     TKTPYPLEWRIGHRVAQACRLRGLLLRPLGNVMALVPPLSISPRELTTMIAILHSAIR
                     EITEELATSR"
     gene            5726..>5928
                     /locus_tag="PLS35_16905"
     CDS             5726..>5928
                     /locus_tag="PLS35_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNI20403.1"
                     /translation="MHETPRRSRIITAGDFFDNVGGGHYNEPILGNMSQDLQSLIRNF
                     SIIAHIDHGKSTLADRLLDATGAV"
BASE COUNT         1245 a         1771 c         1751 g         1161 t
ORIGIN      
        1 tggtcatcgg ggggaagata tgccgacacc gatcacgaga aaaggatacg aagcgctgaa
       61 agccgaattg gaccggctgc ataaagtgga acggccgcgc gtcatcgagg ctatcgccga
      121 agcgcgcgcg catggcgatc tgagcgagaa tgccgaatac gatgccgcga aggaacgaca
      181 gggcttcatc gaagcacgcc tggcagagtt gaagggcaag ctggcggact gccggatcat
      241 cgacattgcc ggccgcacca gcgacaccgt cgtcttcggc gccaccgtcg tgttgatcga
      301 gcaggaagcg caggccaaga aacagtatac cctcgtcgga caggatgaag cggatctcaa
      361 attttcccgc atttcagtgc aatcgccggt cggccgcgcc ctcatcggca agcgggtcgg
      421 ggatttggtc gaggtgacga ctcctgccaa agtggtggaa tacgaagtga tggaaatccg
      481 cttcgaggaa ttatagccgt gatgtcgccc ctcacgtcat tcacgcgttc ctccctcgcg
      541 ccgttccgtt caccgtcgca ggcccggtgg gtctcatgcc ggtaaccatt cccctcatca
      601 cgctgctgac ggatttcggc gaccgcgatt atttcgtggc gagcgtgaaa ggcgtcatcc
      661 tcaacatcaa tccccaagcg cacatcgtgg atctttcgca tcatgtcaca ccgcacgatg
      721 tagccgatgc cgcctatctg ctcaaatcct gctatcgtta tttccccgac ggaaccatcc
      781 acgtcgccgt cgtcgatccg ggcgtcggca caaccaggcg ccccctgctg gtctcgtcct
      841 ctcgctactt ttttatcggc ccggacaacg gcctgttcac ccacatctat caggaagaaa
      901 cgggcgttga agtccggcac ctcgaaaacc ggcaataccg gctcgattcc gaaggcgcca
      961 ccttcgacgg gcgtgatctc ttcgccccgg cggcagcctg gttgaccaaa ggacaaccga
     1021 tcgggtcgtt cggccggctc gtccccaatt acgaacgtct gccgatttcc gaaccgtcct
     1081 gggacaaaca tgtaatggcc ggggagatcg cctatatcga tcgtttcggc aatctgattt
     1141 caaacctcac cgcgtaccat atccgggaag tccgtgggat caccaaacgc gccgatccgt
     1201 acatccgcat cggcggggtc acgatcgact gcctggttca tagttacgga gaggggtcag
     1261 ccgatgctcc ccaggctctg atcaacagca acgggtacgt cgaggtgttc ctcaaggaag
     1321 gtcgcgcggc cgaccggttg aatgtggcca gaggcgcgcg cattgagctc agttaaacaa
     1381 cgccgtgcgc gaccccggcc agactgcttc cgtacctcat gcaatctccc gtaattgaca
     1441 ggcgaaagtt gccggtgttt taatgagatg gtgagcttgc gggccgaaat gcatgacgtg
     1501 gccatcacgg aataggctga tctttctgca tatttcatcc agccggggga gctcaccatt
     1561 ccgggttggt cctgcgtccc gccttcgctg ctcctgtaca ctgatgtgat ccctccagac
     1621 gagtccctgc cgggggcgac cgtttcgagc cggctcaggg gcaggactat ggccgaatga
     1681 cacatcgacc gatatgagat aagaggacac cggttccatg aaaccattgc tgcaacatct
     1741 ttcggtcgtg ctgctctgtc tcaccgtcat gggctgcaca cgccctgatc ctcccgcgcc
     1801 atccgccaaa cctgtacagc catcagtcgt cgccaacggt caaccagaca tccagacaat
     1861 gcgcattgat gcctcgccat ctctgccagc gctgaccctc tcggccagag tgacgtacgc
     1921 agaagacgga tactcaaaaa tttcctcccc acttccaggt ccggtgctcg acgtacgggt
     1981 gaagctcggc caggcggtca aggccggcga catcctgatg gtgatcgacg cggcggatat
     2041 cgctcgcgcc tacgcggcct atgtggagga aatttcggaa ttgggcctgg ccgaacggaa
     2101 ctatgaactg acgaaggatc tctacgacgc ccaggccatg tccttgaagg atctggagca
     2161 tgcggaaaac gatctgaatc gcgagcgggc cgaattcaag caggcgaagg agctgctcct
     2221 ttccctgcgc gttccggccg cggaactcag caaacccctc gcacaacagc aaatcacgtc
     2281 gcgcttcgag ctccgcagtc cgttgacagg caccgtcgtt gaacgcaatg tgacgccggg
     2341 gcaaatcgtg ggaacagctt cggaccagcc gctgttcacc gtggccaatc tggatcgact
     2401 gcaggtggtc gcggatgtgt atgaacacga cctctccggc atcagagtcg gcgccctcgc
     2461 ggccatgacg gtagaagcct atccagccat cgaatttccc gcgaagattg ccgtcatcgg
     2521 cgatgtcgtc gatccggcga ccagaacgat caaaatccgc gccacggttt ccaatcagga
     2581 tcgccgattg aaaccggaaa tgttcgcccg gctgacgatt gccggccaca ccatgcgccc
     2641 gaagattgtg attcctaaac aggcggtgat ggaacgatcc ggcaggcaat ggatcgtagt
     2701 ggagctcgca ccaggccggt tcgaggaacg tcccgtgacc atcgatagcg tgacagacaa
     2761 tcaggtgacg attcgagaag gagtcgtgac cggagaacgc attgttctca gcccctcgtc
     2821 catcaaccac ctggctgcga gcgatacttc tgctggcggg gcctagaccg gctcacggcg
     2881 aagatcggca gcggctacaa tgccgcgaca agaatccgca ccaacccgga caaatcttcg
     2941 gtatcgactc ccgccaccgt ccgattcgtc gtattggtat acgaggcttc cagtgacagg
     3001 gtggtgggat gcccgtaaat cgggttgaga tggaacgcaa acatcccccg gcccacgata
     3061 ctctccgttt cgatcaatcc gtcgctcgac tttgtactgg tgtagccgcc catcgcgctc
     3121 accagtaatc gctggctttg tttgtacagc agagacacgg acgccatcgg cgtctggatc
     3181 ttggcgcccg accagctctg cgtttccgtg cggtaactga atgtcggcgc gaacgtcagg
     3241 gtattgaggg gacgatagga cgcaaccagg gtctgggcaa aggtcgtgga ctgggctccc
     3301 ccccgcatct catcgttcgt caggatgtaa ctcgacgaga gtctggcatt ccaggaaagg
     3361 ccggtatagg cgagggccgc ttccatgctg ttactctggc tgcgctgagc ggtcatgcct
     3421 gcaggaatat agctgctcgc cagggcgctt cgcgcatagg tcaggctcag ctcaggccac
     3481 gccggtttca caagcgcgat gccgatacgg ctcacgtcct gttgaatgcg cggccggttc
     3541 ggctccagat ccacattgtt ccaggtctgc ccggaggaac tgcgcagctt cgcaatgccc
     3601 agcccccatt ctgcccacat ttcccgcacc gtctgatccg gcgagttgaa aaaggctttc
     3661 ccggccgatc gatagttgat gccgtatcgg accggcccgt tggtgccggt cagcgccatg
     3721 cgcaccatgc gtttggcgcc gtcatcacgt tgatcgatgc gggaggtgag gcctgcatcg
     3781 ttggtcgcat tgttggccac ttcgccttct gccgtgagct gccccttgag ccaggtggaa
     3841 ttggcgccga gtcctttggt ttgaggccgt tcggaatcag gcaccaggtc tttcagtctg
     3901 gtggaggtcg tcggcggcgc aaaggacgtt gcttgaaacg acgtatgcat gaccggtgag
     3961 gcaatggaaa agggaatcgc ggcagtcccg gactcgcgag ccgtgtcgct gaactgaatg
     4021 cgctgaagga ggagttccga cggttgctgg tcttcggccc agagtgagaa gggcaggaaa
     4081 aggagcaggc acaggacccg gatgatcagg cccgtcgtca tgaaaccgtg ctcgccgagg
     4141 tgactgcgta gaacagcatg aaatcaagtg tagcactcga ctttcacgct tccggccgcc
     4201 ctggcagtgc acaaaacctg tcgagagact gtaccggcgt gaacaccgaa cggtgactgc
     4261 aggagagcac caatcagcag gaaggacgcg cggtttcagc gcgaggtagc gagttcctct
     4321 gtgatctcac gaatcgcact gtgcaaaatt gcgatcatgg tagtgagctc acgaggcgag
     4381 atggagaggg gagggacgag cgccatgaca ttgccgagcg gccgcagaag cagtccacgg
     4441 agccggcagg cctgcgccac tcgatggccg atgcgccatt cgagcggata cggggtcttc
     4501 gtttgacgat cctgcactaa ctcgatgccg accatgaaac cgcactgacg aatatcaccc
     4561 acatgcggca actcagccag gggttgtaag agacgggcca acaggcgcga tttcttccgc
     4621 acctgggcga gcgtgcgttc gcggcggaag atctccagat tggcgagggc cagagcgcag
     4681 cccagaggat ttcccgtata gctgtgcccg tggaagaacg tcttccactc gtcataccgt
     4741 cccaggaagg cccgatagat ggcgtccgtt gtcagcgtcg cagccaacgg catatatccg
     4801 ccggtcaacc ccttgctgat tgccatcaga tccggcgtga ccccctcatg ctggcaggcg
     4861 aacatcttgc cggtccgtcc gaaaccggtt gccacttcgt cgacgatcag caggacattg
     4921 tatttcgtgc agagctcgcg tacgcgggca agatatcccg ggggggccac catcattccg
     4981 gctgcggcct gcaccagcgg ctcaacaatg acaccagcca gctcgcgatg ccgcgtcttg
     5041 agtaggcgtt cgagcggatc gatgcacgcc atgtcgcacg atggatacgc ccgacccagc
     5101 ggacaacgat aacagtaggg cggttcgacc tgatgcgtgg gaaaaagaag cggcttgaaa
     5161 cgcccatgga acagctcaat gttgcccaca ctgaccgcgc cgatcgtgtc gccgtggtag
     5221 gccattttca aatgcgcaaa ggactgtttt gatccgttgg acggccgagc ttgctgccag
     5281 tattgcactg ccatcttgat cgccacctcg accgccgtcg agccgttgtc ggaatagaac
     5341 acccgccgca accccttcgg ggcaaggcga atgagttcgc gagccagacg aatcgccggc
     5401 ggattcgtga gcccgagaaa ggtcgaatgc gcgatctgcc tgagctgcgc ggtgagggca
     5461 cgatccagcc ggggatgccg atgcccgtga agattcaccc aaatggaagc ggtcccatcg
     5521 agatacttcc gtccttccgt atcgatgaga taggaccctt tgcccttttc gatgatgagg
     5581 gggctctcct gttcccattc ctgcatctgc gtgaacggat gccacacata cgcatgatcc
     5641 caggcggcaa gcggttggcg tgtggatgga ggtgtcatct ggtactgcgg tgtgatgttg
     5701 cgagtgcgcg atcgtagatg cgcccatgca tgaaacgccg cgccgatcgc ggattataac
     5761 ggcgggcgat ttctttgaca acgttggagg cggtcactat aatgaaccga ttttggggaa
     5821 tatgagccag gatttgcaaa gtctcatccg aaatttctcg atcatcgccc atatcgatca
     5881 cggtaaatcg accctcgccg accggctcct cgacgcaact ggcgcagt
//
LOCUS       DAOQEW010000217         5841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5283_length_5841_cov_8.636709, whole genome shotgun sequence.
ACCESSION   DAOQEW010000217 DAOQEW010000000
VERSION     DAOQEW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_5841_cov_8.636709"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..783)
                     /locus_tag="PLS35_16910"
     CDS             complement(58..783)
                     /locus_tag="PLS35_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20404.1"
                     /translation="MGGVLIRPIILTVCIYLFLKFIVPVLPHSAPLPSSLIFLYLMLV
                     MSGSVLFATLSGESKDAFFQPILRFLTGESGGALSSARYLVLALFPLLVGWQAYGSTA
                     SSDAPPGENRTIHPAPPGEYTGLSNPVPKTPENIMYGKGLYASRCAPCHGNFDGKGFA
                     AAGFTPPPANFKDPTTIAMLQESYLFWRIKKGGVGLPVEGMPWKTAMPRWELEMSDED
                     IWKVIMGEYDGAGQKPRTWDESE"
     gene            complement(814..1434)
                     /locus_tag="PLS35_16915"
     CDS             complement(814..1434)
                     /locus_tag="PLS35_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20405.1"
                     /translation="MSEVVKLQLIGLCVVGLGTVILLFIKGQFLRVIGFVAIVLGLFA
                     LVALAVPQMASLPPAEESINIADIKTATDMATIGQKIFFSKGQCALCHSIGPSESARC
                     PDLKGIGAKLSREFIFESLTEPQAYLYLDYRHEGVPKEYPARMPYINRNPIGLSKNEI
                     LSVIAFLQQMSGEPISVSPSELDIPRATPAPVKAADAGTVAVAQAR"
     gene            complement(1501..3360)
                     /locus_tag="PLS35_16920"
     CDS             complement(1501..3360)
                     /locus_tag="PLS35_16920"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI20406.1"
                     /translation="MDNQVRHMAGRIALLAGCAAMLGWIGLPDTAMAQDAAAQAPTAY
                     RDIPYVGSRNLVWIIAQLHLLLAGFVLGVPIFAWLCEVVGWKSGDKRYDKLAKEFTKL
                     LTSSYATTALFGGILLFLLIGFYPKLMAYLTDIFFPSFLVYCGLFLVETATLYLYWYG
                     WDAMSEGNGKKFHIFLGFLLNVFAFFIMIVPNSWATFQASPVVIAEGTDFERAWAATW
                     NPTWWPVNIHRIIANVVLGGYICGAYAGIRYLSAKSTEERYHYDWMGYVGNFIGVFGL
                     LPLPFAGYWLMREIYQYNQQMGITLMGGFLSWLFILQAMLIGVLFLGSNYYFWMGITH
                     RIPGSEGQYRKPIMAMLIILLMCLAVWMTPHSLVASLEEAQKMGGTHHPLLGVFGVMS
                     AKMTVSNLMVLVTFMSFIMYWRAGKEDTAGWAKVAKSVMGAVLVLAGLAVIVLGVWGY
                     FVPAIIRINYFSTSQVAIVIFVMLTITPLTALLLKSAKTTTEMVWGRMPPRAGYSLVL
                     NAVMVILLMTLMGYARSSSRVHWHVYGVMRDSSQYAFSPALGYAAALMALCTFLFCML
                     VAFIFWVATMGDKAKAAASAKGAPEPGHIPAMAGGSPALDGPASDASEGNRPV"
     gene            complement(3410..4411)
                     /locus_tag="PLS35_16925"
     CDS             complement(3410..4411)
                     /locus_tag="PLS35_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20407.1"
                     /translation="MSSFKLGLSILWPACWTALPLKMAFAMLFMAMGTIHLETKLGIT
                     FLMLLMSPVSVFAFFVISLGVGFHFGEGTGLPLLFLVSIPIDIWALGLVARTVFLERL
                     RLEPPDSLGLGLWVRFAIAGAVYLPLLWVVEGGATDLARSIVKAILDMDMLKGLPVAE
                     RIGLEFTAWGSASLVVLIALTFIGLTILGKLVEGKTAGAQPSGDSYQGLISRWDMIRV
                     PADQSLMLTAFTAAGAVLSVLFWMVLPVSTPHPHECCKPASVAPVAPFDPQKSLSKGE
                     KVLKDAEVKIAALEQKTAEDEKDKQKGGKEKAGVKDSSAKSDGKASQVTAGKPAGSK"
     gene            complement(4448..5563)
                     /locus_tag="PLS35_16930"
     CDS             complement(4448..5563)
                     /locus_tag="PLS35_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNI20408.1"
                     /translation="MDTTTQPTTTQPSAIEKVFAFVDERVGLKTLQAKMLNEPVPGGS
                     RWAYVFGSVLLFIFIMQAVTGILLMFYYVPTADHAYASTQYIIHTVDYGWFLLSYHFW
                     GSTAMVVCVFAHMSQVFLWGAYKKPRELIWLVGLALFGIVMGFGFTGYLLPWDQRAYW
                     ATTVGVEIMDKTPLLGDFMARFLKGGATPGQMTLSRFFVIHVMVLPAALMGLAGLHLF
                     LFRKAGPAGPFRGSVEEIKAKTDYFFPRQIWKDIVGMVAVFLCICSLAFWEPIVLLEE
                     ATPDPGDYHPEPEWYFLFLFQLLRLKVFAGEFGQFLGAIALPGVFMALLAALPFIDRD
                     PERNIFKRPIALISWIVVMASILIFTVAAIINREFLE"
     gene            complement(5563..>5841)
                     /locus_tag="PLS35_16935"
     CDS             complement(5563..>5841)
                     /locus_tag="PLS35_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNI20409.1"
                     /translation="HIWTNKKDVPFVAYSGKCPHLGCGFKWRTHKTLGQVFLCPCHLS
                     IYDAAGKVLDGPAPRPLDPLPIKVTATGEITIIDMEFKAGTKAQVRIV"
BASE COUNT         1401 a         1704 c         1599 g         1137 t
ORIGIN      
        1 ctcctcactc gtgccatacg ttccccttaa ttgctcagca gtctctgatc ggagaaatta
       61 ctcggactca tcccaggttc ttggcttctg tccggctccg tcatattcgc ccataataac
      121 tttccagata tcctcgtcgg acatttccaa ttcccatcgc ggcatggccg ttttccatgg
      181 catcccttcg acaggaaggc cgacgcctcc cttcttgatc cgccagaaca gatagctttc
      241 ctgtagcatg gcgatcgtcg tcggatcctt aaagttcgcc ggcgggggcg taaatcccgc
      301 tgccgcgaag cccttaccat caaagttgcc atggcagggc gcacagcgtg aggcgtagag
      361 acccttgcca tacatgatat tctccggcgt cttgggtacc ggattagaca gtccggtata
      421 ttcgccggga ggagcggggt gaatcgtacg attttcaccc gggggcgcgt cactcgacgc
      481 cgtgctcccg taggcctgcc atccgacgag caacgggaac aatgccaaca cgagatatcg
      541 ggcgctactg agcgctccgc cactttcccc agtcaggaag cggaggatgg gttgaaagaa
      601 ggcatctttc gattcaccgc tcagggtggc aaataacacg gagcctgaca tcaccagcat
      661 caggtacaga aaaatcaagc tcgaaggcag cggcgcgctg tgcggcaaca ccgggacaat
      721 gaatttcaag aacaaataga tacagaccgt cagtatgatt ggtctgatga gtacgcctcc
      781 cataccttcc tcctctactt caccgccaat aacttatcgt gcttgggcca ctgccacggt
      841 gccggcatct gcggccttca ccggtgcggg ggtggcacgt gggatgtcaa gttccgaagg
      901 actcacgctg atcggctcgc cactcatttg ctgcaggaac gcaatgaccg acaggatctc
      961 gttcttcgag agaccgatgg gatttctatt gatgtacggc atacgcgccg gatattcctt
     1021 cgggacacct tcatgtcggt agtccaggta cagatacgcc tgcggttccg tcagactttc
     1081 aaagatgaat tcccgcgaca atttggcgcc aatgcctttt aggtccgggc agcgggctga
     1141 ctcgctcggc ccgatcgaat gacagagggc gcactgacct ttgctgaaaa atattttttg
     1201 accgatcgtc gccatgtccg ttgccgtttt aatgtcggcg atattgatgc tctcctccgc
     1261 cgggggtaac gacgccatct gcggcaccgc caacgccacc agggcaaata agcccaacac
     1321 gatcgcgaca aatccgatga ctcggagaaa ttgccccttg atgaacaaaa ggatgaccgt
     1381 tccaagtccg acaacgcaca gaccgattag ctgcagtttg actacttcgc tcataagtcc
     1441 tcaactaagg ataatgatca cgacccgccc ctggttacca gaagaagatc atcttcccga
     1501 tcagaccgga cggtttccct cacttgcatc gctcgccgga ccatccagtg cgggcgatcc
     1561 gcctgccatg gcaggaatgt gacctggttc cggagcaccc ttcgcgctcg ccgcagcttt
     1621 cgccttgtca cccatcgtgg cgacccagaa aatgaaggcg accagcatgc agaacaggaa
     1681 ggtacagagg gccatcaggg cggccgcgta cccgagcgcg ggcgagaagg catattgaga
     1741 agagtcacgc atcacaccgt acacatgcca atggacacgc gacgaggagc gggcataacc
     1801 catcaaggtc atcaggagga tgaccatgac ggcattcaag acgagtgagt agccggcgcg
     1861 aggcggcata cggccccaca ccatctcagt ggtggtctta gcgctcttga gcaataacgc
     1921 cgtcaacgga gtgatggtca acatgacgaa gatgacgatc gcgacctggg acgtcgagaa
     1981 gtaattgata cgaataatgg ccggtacgaa gtagccccac acaccgagca cgataaccgc
     2041 gagtccggca aggaccagca cagcgcccat cacagacttc gccactttgg cccatcccgc
     2101 agtgtcttct ttgcctgcgc gccagtacat gatgaagctc ataaacgtca cgagcaccat
     2161 caggttcgat accgtcattt tggccgacat gacgccgaac acgccgagga gcgggtggtg
     2221 tgtgccgccc atcttttgcg cttcttccaa acttgcgacg agggagtggg gtgtcatcca
     2281 caccgcaaga cacatcagga ggatgatcag catcgccata atcggtttgc gatattggcc
     2341 ctccgagcct ggtattcgat gcgtaatacc catccagaaa tagtagttgg agccgaggaa
     2401 gagaacaccg atcaacatgg cttgcaggat gaacaaccag gagaggaatc cgcccatgag
     2461 cgtgatgccc atctgttgat tgtactggta gatttcacgc atcaaccaat agccggcgaa
     2521 gggcaggggc aacaagccga agacgccgat gaagttgccg acgtagccca tccaatcgta
     2581 gtggtaccgt tcttccgtgc tcttggccga caggtaccga atacctgcgt aggctccgca
     2641 aatgtagcct cccagcacca cgttggcgat gatccggtga atattcaccg gccaccaggt
     2701 tggattccag gtcgccgccc aggcccgctc gaagtcggtg ccttcggcaa tgacgaccgg
     2761 gcttgcctgg aaggtcgccc aggagttcgg aacgatcatg atgaaaaagg cgaacacatt
     2821 gagcaggaag cccaaaaaga tatggaactt cttcccattt ccctcggaca tcgcatccca
     2881 gccgtaccaa tagaggtaga gggtcgcggt ttccacgagg aagaggccgc agtagacgag
     2941 gaaggatggg aagaaaatgt cggtcaaata ggccatgagc ttcgggtaga agccgatcaa
     3001 caggaacagc aggatgccac cgaacaacgc ggtagtggcg taggatgacg tcaggagctt
     3061 ggtgaactcc ttggcgagct tatcgtagcg cttgtcgccg gacttccaac cgaccacttc
     3121 gcacaaccag gcaaaaatgg gtacgccaag cacgaatccg gcgagaagga ggtgtaactg
     3181 ggcgatgatc cagaccagat tgcggcttcc gacatagggg atgtcacgat aggcggtggg
     3241 cgcttgagca gccgcgtcct gagccatagc cgtgtccggc aagccgatcc agccgagcat
     3301 cgccgcgcat ccggccagca acgcgatacg gcctgccatg tggcgcacct gattatccat
     3361 agtccgccct tggttcctca ttgttctcac ctcgtcgttc gccatgatgt catttgcttc
     3421 ccgccggttt cccggctgtt acctgtgaag ccttgccgtc gctcttggcg gagctgtcct
     3481 tgacaccggc tttttccttg ccgccctttt gcttatcttt ctcgtcttca gccgtcttct
     3541 gttctaacgc ggcgatcttc acctccgcgt ccttcaacac cttttccccc ttggagagag
     3601 atttttgggg gtcgaatggt gcaacaggag caacgctggc tggcttacag cattcgtgag
     3661 gatgtggagt ggagactgga agcaccatcc agaacaacac gctcaggacc gccccagctg
     3721 ccgtaaaggc ggtgagcatc agactctgat ccgccggcac gcgaatcata tcccaccgag
     3781 agatcagccc ctgataactg tcccctgacg gctgggctcc ggctgtcttt ccttcgacca
     3841 acttgccgag aatggtcagt ccgataaagg tcagggcaat caacacgacc agagaggcac
     3901 tgccccacgc ggtaaactcc aggccgatgc gttcagccac tggaagtccc ttcaacatgt
     3961 ccatgtcgag aatggccttc acgatcgaac gggcgagatc cgtggctcct ccctccacca
     4021 cccagagcag gggcagatat acggccccgg caatagcgaa acgaacccat aaccccaacc
     4081 cgagtgaatc cggtggttca agcctgaggc gctccagaaa tacggtacgc gccacgaggc
     4141 ccagcgccca gatatcaatg gggatggata ccaggaagag taagggaagc ccggtgcctt
     4201 caccaaagtg aaatccgacg cccaaggaaa tgacgaaaaa tgcaaacaca ctcaccgggc
     4261 tcatgagcag catcaggaaa gtaatcccga gcttggtctc cagatggatg gtgcccatag
     4321 ccataaacaa catggcgaat gccatcttga gcggcagggc ggtccaacag gcaggccaga
     4381 gaatgctcag ccccaattta aatgacgaca tcgtctctcg accagtcatg tgacgaagac
     4441 cctccggcta ttccaaaaat tcgcgattga tgatcgccgc cacggtgaaa atgaggatac
     4501 tcgccatgac tacaatccag ctgatcaagg caatcggccg tttgaaaata tttcgttctg
     4561 ggtcgcgatc aataaagggc aaggccgcca gcaaggccat gaagaccccg ggcaacgcaa
     4621 tcgcgcccag gaactgtccg aattctcccg cgaagacttt cagacgtaac agctggaaca
     4681 gaaacaggaa gtaccactca ggttccggat ggtaatcacc gggatccggg gttgcctcct
     4741 cgagcagcac gatcggttcc cagaaggcca agctgcaaat gcagaggaac acggccacca
     4801 ttccgacaat gtctttccag atttgtcgcg ggaagaaata gtcggtcttg gctttgatct
     4861 cctcgacgct gccacggaac ggaccggcgg ggccggcttt ccggaacagg aagaggtgca
     4921 aaccggccag acccatgagc gcagccggca agaccatgac atggatgacg aagaagcggc
     4981 tcagcgtcat ctgtcccggc gtcgccccac ccttcaggaa ccgggccatg aagtcgccca
     5041 gcaacggcgt cttgtccatg atttcgacgc cgacggtcgt ggcccaatag gcccgctggt
     5101 cccagggaag cagatatccg gtgaagccga atcccatcac gatgccgaac agggcgagac
     5161 cgaccaacca gatgagttcg cgaggctttt tgtatgcgcc ccaaagaaag acctgggaca
     5221 tgtgcgcaaa cacgcagaca accatcgccg tggagcccca gaagtggtaa ctcaggagga
     5281 accatccata atccacggtg tggatgatgt actgtgtgct ggcgtacgcg tggtccgcgg
     5341 tcggcacgta atagaacatc aacagaatgc cggttaccgc ctgcatgatg aagatgaaga
     5401 gcagcaccga gccaaaaaca taggcccacc gtgagccgcc ggggacaggc tcgttgagca
     5461 ttttggcttg caacgtcttc aacccgacgc gttcgtcgac aaaggcgaag actttttcaa
     5521 tggctgaggg ctgcgtggtc gtcggctgcg tagtcgtatc cattagacaa tccgaacctg
     5581 ggccttagtg cccgccttga attccatatc aataatggtg atctcacccg ttgcggtaac
     5641 tttgatgggc agggggtcca acggtcgtgg agccgggcca tccaggacct tccccgcggc
     5701 gtcgtaaatg ctcaagtgac acggacagag aaagacctga ccgagggttt tgtgcgtccg
     5761 ccacttgaat ccacaaccca gatgcggaca cttgcctgaa taggcgacaa acggaacatc
     5821 cttcttattc gtccagatat g
//
LOCUS       DAOQEW010000218         5833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5298_length_5833_cov_6.967290, whole genome shotgun sequence.
ACCESSION   DAOQEW010000218 DAOQEW010000000
VERSION     DAOQEW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5298_length_5833_cov_6.967290"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLS35_16940"
     CDS             complement(<1..328)
                     /locus_tag="PLS35_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI20410.1"
                     /translation="MVTKRKALAKRSASAGRKPVRFEDLTVKDIVNQRVQSARLDLTG
                     MKVASLLLKAGGTVPVIDTSKQLLGVVSEHDVLLALDEGQAWGTRTVRDLMSANPYSV
                     RPETSLP"
     gene            complement(371..907)
                     /locus_tag="PLS35_16945"
     CDS             complement(371..907)
                     /locus_tag="PLS35_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20411.1"
                     /translation="MVKTYLMALVGGCMVMSLALTADASAGGSTVVAHVLSDEEAVEL
                     EIPTDIGVQKERLPLYRLGAVRYFSAGIGQVERTASYPLFSLKLVFTAGGKPFVSGVA
                     VTLRQPKDGTVLTIPGEHNTGPWLFIDLPDGTYQISATLGGVTQQVNNLTVRRGHVIT
                     QHVRWAEDRSPALPAQAE"
     gene            1234..2139
                     /locus_tag="PLS35_16950"
     CDS             1234..2139
                     /locus_tag="PLS35_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20412.1"
                     /translation="MNMLIAVDGSEFAEWSVQMLEAIAGRPPDTVTLLHVVDSASLKS
                     SARKHAAVSKQAIAAMTKAGDQVLRRFEGLAKTALKQATTKPRTTIDTLLAHGRVADT
                     IAKVAKQKKADLLVVGSRGLSDAEHYLLGSVSRKVSALAPCPVLVVKRPLTSLSHVLV
                     AADTSKHSQGACSFLCKQFLPDSARVTVLSVVEPAVTELASKYLSRDQVEQISAPKRQ
                     AAEQLVEKLRARFLAEGYAVTPEVQFDHVTDALLRQATSRSIDLLVAGSRGLTGSERL
                     QLGSVSETLLKYAPCSVLIVRGWRA"
     gene            2132..4912
                     /locus_tag="PLS35_16955"
     CDS             2132..4912
                     /locus_tag="PLS35_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNI20413.1"
                     /translation="MPELTALEICRLAPSQVFRALATSPQGLSADDVRRRTLKHGPNS
                     LQALRRTPLIGRFARQFTHFLALLLWVAAGLAFLADAFHAGEGMATLGRAILGVILIN
                     AIFAFFQEYRAERAVQALRGMLPAKAWVIRDGQQQQIARSELVPGDVLVLEEGEQVPA
                     DARLTEVSGMRMDNSSLTGESKPQRRTAEPITDGHPLDIANLAFAGTTVLSGHGQAVV
                     FATGLSTEFGKIAHLTTSVQSGLSPLQQEIVKVTHVVAGLSVAMGLVFFAIGVSMGLG
                     FLSSAMFGIGIIVANVPEGLLPTVTLALAMGSQRMAARKALIKHLASVETLGCTTVIC
                     TDKTGTLTENRMRLDKLYVDDLIVESRESCLFSRNRLISAAESERWRPLFDAMIHCNN
                     AKRTRRPDGRSQATGDPTETALLEFAADHGLLHRPPLRRMGEWPFDADRKRMTTLHWS
                     EGRLLAFTKGAPESVLPRCTMQQGSSAAAELTPDGRKKILAQSQTFARQAYRVLALAM
                     REVERGVDELEADTLEQGLTFLGLVAMMDPPHREVPDAIQKCRKAGIRVVMITGDHPM
                     TAQAIASKIGLAPETPVAQPGRFVPVIEGAQVDTFTDEQLRRLLTPTAPGEPDPIFAR
                     MAPRHKMRIVSVLKEMREVVAVTGDGVNDAPALKMADIGIAMGAAGTDVAKETASMIL
                     LDDNFATIVSAIEEGRAVFLNIRKFMTYVLASNVPEVVPYLAYGLSSLPLALTVPQIL
                     AVDLGTDMVPALALAAERPHSGVMDEPPRPRTERLLNRDVLIRAYAFLGLIEAGIAMG
                     GFFLYLHSEGWTWGDPLDWNSPLYKEATTVTFAGIVAAQVANVFACRSDRFPAFQLGW
                     VSNPLLLLGVAVELTLLFIMTYSPLGHLILGTASIPAWIYGPLVLGAIGLLLADGFKK
                     SLSGRMRTRQAG"
     gene            5013..>5833
                     /locus_tag="PLS35_16960"
     CDS             5013..>5833
                     /locus_tag="PLS35_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI20414.1"
                     /translation="MTTAHGSHVSDYMHRQLEVVPQDTSVVTVATRMRTRSIGSVLIE
                     SFDRPHNDCRIAGIVTETDLVSKVLAAGRVPSRTSMADIMSSPLITIAPDRPMVDASH
                     LMQGKNVRHLVVTEGTDVLGVISVRDLVRHFVDADGGPVQALTNVYRPLSVLMQKAIE
                     TIGSEETAMAAAQRMADKHIGALFVIEADELVGIITEGDLVRKLLAYQLDPEAMRVGA
                     LMNSPLLDIDINRTIRDASERMAAKRIRHLAVTEHEKIVGVLSIRDLVKMVAIRDR"
BASE COUNT         1146 a         1882 c         1711 g         1094 t
ORIGIN      
        1 tgggcaaact ggtctcaggc cgcaccgaat agggattcgc gctcatcaga tcccgaaccg
       61 ttcgagtgcc ccacgcctgc ccctcgtcaa gggccagcag gacatcatgt tcactcacca
      121 cgcccaacag ttgtttcgat gtgtcgataa ccggcacggt gccccctgct tttagtagca
      181 acgacgctac cttcatgccc gtgagatcaa ggcgagccga ctggacgcgc tggttcacaa
      241 tgtctttcac cgtcaggtcc tcgaaccgta ctggtttgcg gcctgcgctc gccgatcgtt
      301 tcgccagggc cttgcgtttc gtcaccatgc ttcctcctcc tgatcgaccc atgtattgtg
      361 accttcattg ctactcagct tgcgcaggca atgcagggct ccggtcctcg gcccagcgga
      421 catgctgcgt gatgacatga cctcgccgca cggtgagatt gttgacctgc tgcgtgacgc
      481 cgcccaacgt ggccgatatc tggtacgtgc cgtcaggcag gtcgataaac aaccacgggc
      541 ctgtgttgtg ctcacctgga atcgtcaaca ccgttccgtc tttgggctga cggagcgtca
      601 cggctacccc cgagacgaag ggcttccccc ccgccgtgaa gacaagcttc aacgaaaaga
      661 gcggataact ggctgtccgc tcgacctgcc cgatcccggc actgaagtac cggactgcgc
      721 ccaggcgata caggggaaga cgttctttct gcacaccgat gtccgtcggg atctcaagtt
      781 cgacggcctc ctcgtcggaa agcacatgag cgaccaccgt cgagccaccg gcgctcgcat
      841 ccgccgtcag cgcgaggctc atgaccatac acccgccgac caatgccatg aggtacgtct
      901 tcaccatgtc ggttcctcct actcgattgt cacgcaatgg tgatgcctga gtacggcgcc
      961 ctgtgttcga acagaaatcg cttacgaggc cgcatcagat cacaccgtca gcccaccgca
     1021 aacccttgaa ctgaggccaa ttaccccaaa ctgacacccc acactgctgc aaaacgccgc
     1081 agcgccacga tcgcggcacg atccccgaga cgaggccaag gccgctcacc gttcgacagc
     1141 gtatggaatg gcttgctaga gtttgatctc gttcaggcat atccgatgca gactacattc
     1201 caggatagag tcaggccatg gaggaatgca gcgatgaaca tgttaattgc ggtagacgga
     1261 tcggaattcg ccgaatggag cgtgcaaatg ctggaagcga tcgccggacg cccgccggat
     1321 accgtgacct tgctgcatgt cgtcgacagc gcgtcgctga agtcctcagc ccgcaaacac
     1381 gcggctgtct caaagcaggc catcgccgca atgacgaaag ccggcgatca ggtgctgcgc
     1441 cgcttcgaag gcctggcgaa aaccgcactg aagcaggcca ccaccaaacc tcgcaccacc
     1501 atcgacacgc tgctcgccca tggccgcgtg gcggacacga tcgcgaaggt ggccaagcag
     1561 aaaaaagccg acctgctcgt ggtgggctcg cgcggcctca gcgatgcaga gcattacctg
     1621 ctgggcagtg tctcgcgcaa agtcagcgca ctggcgccct gcccggtgct cgtcgtgaaa
     1681 cggcctctca ccagcctctc ccacgtgctc gttgcggcag atacgtcaaa acattcgcaa
     1741 ggcgcctgca gcttcctgtg taaacaattc ctcccggatt ccgcccgggt cacggtcctt
     1801 tccgtggtcg agcccgccgt caccgaactg gcgagtaagt acctgtcacg cgaccaggta
     1861 gagcaaattt ccgcgcccaa gcgacaagcg gctgagcagc tggtggagaa gttgcgcgcc
     1921 cggtttctgg ccgaagggta tgcggtcacg ccggaggtgc agtttgatca tgtgactgat
     1981 gcgctcctcc ggcaggcgac ctcgcgctcc atcgacctgc tcgttgccgg ctcgcgggga
     2041 ctgacggggt cggaacggct gcagctggga agcgtatcgg aaacgctcct gaagtacgcg
     2101 ccttgctcgg tcctcatcgt gcgagggtgg cgtgcctgaa ctgacggcgc tagaaatctg
     2161 ccgcctggcg ccgagccagg tgtttcgtgc attggccacc tcgccgcaag gcctctccgc
     2221 ggatgatgtg cggcgacgaa ctctcaagca tgggccgaat agcctgcagg ccttgcgccg
     2281 cacgccgctc atcggccggt ttgcgcgcca attcacgcat ttcctcgcgc tgctgttgtg
     2341 ggtggccgcc ggcctcgcct ttcttgccga cgccttccac gccggggagg gtatggccac
     2401 cctgggtcgg gccatcctcg gcgtgattct catcaacgcc atcttcgcct tcttccagga
     2461 atatcgagcg gagcgggccg ttcaggcctt gcgcggcatg ttgcccgcca aagcctgggt
     2521 gattcgcgac ggccaacagc aacagatcgc ccgcagcgaa ctcgtacccg gcgatgtgct
     2581 cgtgctggag gaaggcgaac aggtgcccgc cgatgcgcgg ctcaccgagg tcagcggaat
     2641 gcggatggac aactcgtccc tcaccggtga atcgaaaccg caacggcgga cggctgagcc
     2701 catcaccgat ggccacccgc tcgacattgc gaatttggcg tttgcgggga caaccgttct
     2761 ttcaggccat ggccaggccg tcgtctttgc gaccggcctc agcactgaat tcggcaagat
     2821 tgcgcatttg acgacgtccg tccaatccgg gctcagcccg ctccagcagg aaatcgtgaa
     2881 ggtgacgcac gtcgtggccg gcctgtccgt cgccatgggc ctcgtgtttt ttgccatcgg
     2941 cgtcagcatg ggtctgggat ttttgagcag cgcgatgttc ggcatcggca tcatcgtcgc
     3001 caacgtccct gaaggactcc tgccgaccgt caccctggcc ctggccatgg gcagccagcg
     3061 gatggccgcg cgcaaagccc tcatcaaaca cctcgcctcc gtcgaaacac ttggctgcac
     3121 cactgtcatc tgtacggaca agaccggcac gttgaccgaa aatcgtatgc gcctcgacaa
     3181 actttatgtc gacgatctca ttgtcgaatc acgggagagc tgcttgttct cgcgaaaccg
     3241 ccttatcagc gccgctgaat ccgaacggtg gcgtccgctg ttcgacgcca tgatccattg
     3301 caacaatgcc aaacggacgc gccgcccgga tgggcggagc caggccaccg gggatccgac
     3361 agaaacggcg ctcctggagt ttgccgcaga ccatgggttg cttcatcggc ccccgctgcg
     3421 gcgcatgggt gaatggccct tcgatgccga tcgcaagcgg atgacgaccc tgcactggag
     3481 cgagggccgc ttactcgcgt tcaccaaagg cgcgccggaa tcagtcctcc cgcgatgcac
     3541 catgcagcag ggatcctcag ccgccgcgga actcaccccc gacggacgga aaaagattct
     3601 ggcacagagc cagaccttcg cgcggcaagc gtaccgcgtg ctcgccttgg ccatgcgtga
     3661 agtggaacgc ggcgttgatg aactggaagc cgataccctc gaacagggcc tgacgttcct
     3721 cggactcgtc gccatgatgg acccgccaca ccgcgaggtg ccggacgcca tccagaaatg
     3781 ccgaaaggcc gggatccgcg tcgtgatgat taccggcgat catccgatga ccgctcaggc
     3841 catcgcgagc aaaatcggcc tggcgccgga gactcccgtc gcgcagccgg ggcgattcgt
     3901 gccggtgatt gaaggcgcac aggtggacac cttcactgat gagcaactcc gccgccttct
     3961 cacgccaacg gctcccggcg aaccggaccc catcttcgcc cgcatggcgc cccggcataa
     4021 gatgcgcatt gtgtccgtgc tgaaggagat gcgcgaagtg gtggcagtga ccggcgacgg
     4081 cgtgaatgat gcgccggcgc tgaagatggc agacatcggg attgccatgg gggcagccgg
     4141 aaccgacgtg gcgaaggaga ccgcctcgat gattttgctg gacgacaatt ttgccaccat
     4201 cgtcagcgcc atcgaagagg gccgggccgt gtttctcaac atccgcaaat tcatgaccta
     4261 cgtgttggcc agcaacgttc ccgaggtcgt gccctacctg gcgtacggac tctcgtcgct
     4321 tccgctggcg ttgaccgttc cgcaaattct cgcggtcgat cttggaacag acatggtgcc
     4381 cgccttggca ctggctgcgg aacggcctca cagcggcgtg atggatgaac ccccgcgacc
     4441 aaggacagag cgcctcctca accgggacgt actcatacgg gcctatgctt ttctaggcct
     4501 gatcgaagcc ggcatcgcca tgggcggctt cttcctttac ctccacagcg agggctggac
     4561 ctggggtgat ccattggact ggaactcccc cctgtacaag gaagcaacaa ccgtcacctt
     4621 cgccgggatc gtcgcggccc aggtggcaaa cgttttcgcc tgccggtcgg accgcttccc
     4681 ggccttccaa ctcggttggg tcagcaatcc attgctcctg ttgggagtgg ctgttgaact
     4741 gaccctcctg ttcatcatga cctacagtcc gctcggacat ctgatcctcg gcacagcctc
     4801 catccccgcc tggatctatg ggccgttggt cttgggagcc atcggacttc tgctggccga
     4861 tgggttcaag aaatctctga gcgggcgcat gcgaacccgg caggctggat gatgaaggga
     4921 ctgagccatg aacaattccc gctgattgaa cgaccatcgg caccggttgc acagtgccgc
     4981 cacaggacga ctctccacga aaggacgttc gtatgacgac cgcacatggt tcgcacgtca
     5041 gtgattacat gcaccggcag ttggaagtgg ttccgcagga tacctcggtg gtgaccgtcg
     5101 ccacgagaat gcggacacgc agcatcggct cggtgttgat cgaatccttc gaccgccctc
     5161 acaacgattg ccggattgcc ggcatcgtga ccgaaaccga tctcgtctcc aaagtcttgg
     5221 ccgccggccg ggttccgtct cgaaccagca tggctgacat catgagcagc ccactcatca
     5281 cgattgcacc ggatcgaccg atggtcgatg ccagccatct catgcaaggc aagaacgtgc
     5341 ggcatctggt cgtgaccgaa gggacggacg tgctcggcgt catttccgtc cgtgatctcg
     5401 tgcgacattt cgtagatgcc gacggcgggc cggtacaggc actgaccaac gtctatcgcc
     5461 cgctgagcgt gctcatgcaa aaggcgattg aaaccatcgg cagcgaagaa accgccatgg
     5521 ccgccgcaca acggatggcc gacaaacaca tcggggcgct ctttgtaatc gaagcggacg
     5581 aactcgtggg tatcatcacc gaaggtgacc tcgtgcggaa gttgctcgcc tatcagctcg
     5641 atcccgaagc catgcgagtg ggcgccctca tgaactcgcc cttactcgac atcgacatca
     5701 accgcacaat ccgcgatgcc agtgaacgga tggcggctaa acgcattcgt cacttggctg
     5761 tgaccgagca tgagaagatc gtcggggtcc tctctatcag agacctggtg aagatggttg
     5821 cgatccggga ccg
//
LOCUS       DAOQEW010000219         5830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5301_length_5830_cov_7.179913, whole genome shotgun sequence.
ACCESSION   DAOQEW010000219 DAOQEW010000000
VERSION     DAOQEW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5301_length_5830_cov_7.179913"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3698
                     /locus_tag="PLS35_16965"
     CDS             <1..3698
                     /locus_tag="PLS35_16965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20415.1"
                     /translation="PDVRLLAGLEPADVASALAAADLVVLASHAEVSPLCLLEAMSVG
                     RPWLATPDCGTAAEHAGGLVLPLSQFASAVGLLKKHPELCAELGRLGREHWAQSHDWA
                     TVLTAWEDLIEGRPVSHSFGTPVSIAERRETLREAFRRLLAQEGTAAASTASTLQVET
                     KGVVMDGLNQGPRGSVDRLAISNSAPTPQEQKQQDQFYIDMFVKSRGWSSPGPNHDEA
                     ARWSKIAAFLEYLLRRVRQTDPQRQLRMLDVGCGRGWLTNLATAYGTCEGIEPVAGVI
                     EHARRLFPHLRFEAGTAQSVLSRPDFAPYDVILCSEVIEHIPHGRKEGFVAELSMLLK
                     PDGYVILTTPRGEMWEQWKTIAPPCQPVEDWVTEAQLRDIFVSQGFCELGLERVHCEL
                     PSLRYIPAPTPADFHKLNLVPIYQIWACQRRGASPVPSFTRPPMVSVIVPTYNRPDRL
                     QVALSSILAQTYQDFEIIVVNDGTVDVSDVIAPLNRDGRITVIRHDRNRGLAAARNTG
                     IRAAKGTYIAYLDDDDTYLPDHLQTLVTTLQGGEHKVAYTDAWRIHEVRQGDQYVVTG
                     QDIPYSRDFNCIDLLLCNYFPVLCVMHEKACLEQVGVFDESLFAHEDWDLWIRMATIY
                     PFLHIKHTTAAFTWRRDGSSMTSSTSDTYRRTTEIIYRKYRPYAERIAGVLEAQQKKL
                     EGMRSDAQAKTFDCSIIIPVWNNLALTTQCLTALAEVTHGVSYEVIVVDNHSTDDTPA
                     FLAGLGGDVKIITNNDNLGFAKACNQGAQAAKGEYLVFLNNDTIPQAGWLSALVEEVK
                     AHSEVAVVGSKLLYEDGTIQHAGVAFSREFLMPYHMYPGVPADAPFVSRRRELQCVTA
                     ACMLIRRQVFAQVGGFDEGYKNGFEDVDLCLKVGEKNWKIVYQPQSTVIHLESRTPGR
                     KAHEEENTLRFRERWGRCWWVTDEDRLHFDDGYSVHTHLKDGLLGYTLYLITDPAIRA
                     ERAFVADVQRATAVKDQQKIVSLLKRIEQWPTDAWILRWGALLSEGIGHLELALSFWK
                     RVISLKNDPQARLALAKQALESGAMKEADHHVTALLQAEPSHGEGWLLRGILAMQLNA
                     YRDAEQAFEQAKQAGAHPRKAMLGLVMAAMGDNRPEAAWSHVAGLCADYPDDEECMHW
                     MLKCGTLLQRWDALATRLSSFVVRNPGNIAMRFALAGVLLRAGRRADAQREYDWLRAM
                     VPTFEGMDELAKQLAESERPLVPNHAA"
     gene            3733..4881
                     /locus_tag="PLS35_16970"
     CDS             3733..4881
                     /locus_tag="PLS35_16970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNI20416.1"
                     /translation="MARLGDLVQTLPAIESLQEAYPKRTLDVLCAAPLSSLLAGVSHI
                     GRVIPWDGAQWRDWATGWPENPTVTLQVMQQYLASLGDTTYERVYPLNQHERSHLMTQ
                     LFLSHSTREQEHDRMEARILPWAQHLRHVARERGANRVHLADAWCGMCGVLPRGQAPS
                     CEPPPVELPQDLAEIGERQGRWIAVVTGAGEADRCIPPAVWSQWIREFLAQTQDGQVV
                     LIGSGTERETAQAILDSVPTLLHGRVWDATGRTTISQLMRVLQQCHWVIGADTGPLHL
                     GTLAGARALGWYFSRARMHETGPYGEGHWVYQHATQAQPQRWPIQESIALMCGDQGRR
                     ATDWTLWRSGMDRWGAYFDDGSGSATVESSRADIWRACAPALCEPMAA"
     gene            4878..>5830
                     /locus_tag="PLS35_16975"
     CDS             4878..>5830
                     /locus_tag="PLS35_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20417.1"
                     /translation="MNMFQDNLNRLASRHRDLAAAVKSAAGGVLTIEPARTGLPSARR
                     SGRWIHSAYDPLREAQTWADTHAAACKSGEMVVVAGVGLLYHVEALRKKLAPDVSLAV
                     LLPNLDEFHDALVTRSLEGWSESILWLPGTPAEIAETLATIGRPIRCLSYTPAALADA
                     EFHRAVEDALRQGIARQAGGQLTIALVGPIYGGSLPITGYVKRALETLGHKVQLIDHS
                     IHAASYEVMGALKDPRNRQLMQGRMADVLSQWTLATLAESPPDLVLSMAQAPMTLQVL
                     EHLRKKKFLTAMWFVENYRHLTYWQQLAPGYEFWFVFQQGA"
BASE COUNT         1192 a         1704 c         1769 g         1165 t
ORIGIN      
        1 gaccggatgt ccgactgctt gcgggactgg agcctgccga tgtcgcctca gccctagcgg
       61 cagccgatct cgtggtgttg gcctcgcatg cggaagtctc tcctctctgc ctactggaag
      121 cgatgagcgt cggtcggccc tggctcgcga cgccggactg cggaaccgca gccgagcatg
      181 cggggggcct ggtgcttccc ctgtcccagt tcgcctccgc cgtaggactg ctgaagaaac
      241 atccggagtt gtgcgccgag ctcggacgac tgggccgtga acattgggca cagtcgcatg
      301 actgggcgac cgtcctgacg gcttgggagg acctgatcga agggcgtccg gtgtcacatt
      361 catttggcac accagtctcc atcgccgaac gacgagagac tctccgcgaa gcattccgtc
      421 gtcttctggc acaggaaggc actgccgcag cgtcgactgc gtccacgctg caggtcgaaa
      481 cgaaaggggt cgtgatggac gggttaaacc agggaccgcg aggctcggta gaccgactcg
      541 ccatatcgaa ttccgccccg acgcctcaag aacagaaaca gcaggaccaa ttttacatcg
      601 acatgttcgt gaagagccgt ggctggtcat cgccaggccc caatcatgac gaagcggctc
      661 gttggagcaa aatcgccgcc tttctggaat atctgctccg tcgcgtgcgc cagacagacc
      721 ctcagcggca acttcgcatg ttggatgtcg gatgtggacg cggctggctg acgaatctgg
      781 cgacggccta tggcacatgc gaaggcatcg aaccggtagc aggcgtgatc gagcatgcac
      841 gacggctatt tccgcacctg cgattcgaag caggtactgc ccagagcgtg ctcagtcgac
      901 cggactttgc tccgtatgat gtcatcctct gttctgaagt catcgaacat attccacacg
      961 gccggaaaga agggtttgtc gcagaattga gcatgcttct gaagccggat ggctatgtca
     1021 ttctgacgac accgcgcggg gagatgtggg aacagtggaa aaccatcgcg ccgccttgtc
     1081 agccggtcga ggattgggtc acggaagcgc aactgcgcga catcttcgta agccaagggt
     1141 tctgcgaact cggcctcgaa cgggtgcact gcgaacttcc ttcgctgcga tatatccctg
     1201 cgcccacccc ggcagatttc cataaactga acctggttcc catttatcaa atctgggcct
     1261 gtcagcgtcg gggtgcctct cccgttccct cgtttacacg cccgccgatg gtctcggtca
     1321 ttgtgccgac ctacaatcga ccagatcgtc tgcaggtggc gctctccagc atcctggcgc
     1381 agacctatca ggattttgag atcatcgtcg tcaacgacgg cacggtcgat gtgagcgacg
     1441 tgatcgcccc cctcaaccgg gacggtcgta tcaccgtgat ccggcacgat cgcaaccgtg
     1501 gactggcggc ggctcgcaat acgggaatcc gggcagccaa aggcacctac atcgcgtatc
     1561 tcgacgacga tgacacctat ctcccggacc atctgcagac gctggtcacg acattacagg
     1621 gcggcgagca caaggtcgcg tacacggacg cctggcggat ccatgaagta cggcagggtg
     1681 atcagtacgt ggtgaccggc caggatatcc cgtattcgcg cgacttcaac tgcatcgatc
     1741 tcctgttgtg caactatttc ccggtgctct gcgtcatgca tgagaaagcc tgcctcgaac
     1801 aggtcggcgt cttcgacgaa tcgctcttcg cgcacgaaga ctgggatctg tggatccgga
     1861 tggcgacgat ctacccgttt ctccacatca agcacaccac cgcggcattc acctggcgtc
     1921 gcgatggctc atcgatgacc agcagcacca gcgacaccta ccggcgcacg accgaaatca
     1981 tctatcgaaa ataccgaccc tatgccgagc gcatagcggg ggtgctggag gcgcaacaaa
     2041 agaaattgga aggcatgcga tccgacgccc aggccaagac gttcgattgc tccattatca
     2101 ttcccgtgtg gaacaacctg gccctcacca cgcaatgttt gacagccctg gcggaggtca
     2161 cacacggtgt gtcttacgaa gtcatcgtgg tggacaatca ctcgacggac gacacccctg
     2221 cgtttctggc cggtcttggc ggcgatgtca aaatcatcac caacaacgac aatctgggat
     2281 ttgccaaagc ctgcaaccag ggggctcagg cggccaaggg tgaatacctc gtgttcctga
     2341 acaacgacac cattccacaa gccgggtggc tcagtgctct ggtagaagag gtaaaagccc
     2401 attccgaggt ggcggtggtg ggaagcaaac tgttgtacga agacggcacg attcaacatg
     2461 cgggagtcgc cttctcgcgt gagttcctga tgccctacca tatgtatccg ggagttccgg
     2521 cagacgcacc ctttgtctcg cgccgccgcg agctgcaatg cgtgaccgcc gcctgcatgc
     2581 tcatccgccg gcaggtattc gcgcaggtcg ggggattcga cgagggctat aagaacggat
     2641 ttgaagacgt cgatctctgt ctcaaggtcg gcgagaagaa ttggaaaatc gtctaccagc
     2701 ctcagagcac ggtcattcat ttggaaagcc gcacacctgg acggaaggcg cacgaggaag
     2761 aaaacaccct tcgttttcgg gagcgctggg gccgttgctg gtgggtgacg gacgaggatc
     2821 ggctgcattt tgacgatggc tattctgtgc atacccatct caaagatggc ctccttgggt
     2881 acacgctgta cctcatcacc gatcctgcga ttcgagcaga acgggcattc gtcgccgacg
     2941 tgcagcgagc gacagccgtg aaggatcagc agaagattgt ctccttgctc aaacgaatcg
     3001 aacagtggcc caccgatgca tggattcttc gttggggcgc cttactaagc gagggcatcg
     3061 gccatctgga attggcgctt tcgttctgga aacgtgtgat cagtctgaaa aatgacccgc
     3121 aggctcgctt ggcgttagcg aagcaggcac tggaatcagg cgccatgaaa gaagcggatc
     3181 atcatgtgac cgccttgctg caggcagaac cgtcacacgg ggaaggctgg ctcctgcgcg
     3241 gcattctggc catgcagctc aatgcctacc gagacgcgga acaagccttc gagcaagcga
     3301 aacaggcagg cgcacatccc cggaaggcca tgctcggcct ggtcatggca gccatggggg
     3361 acaatcggcc tgaggccgcc tggtctcacg tggcaggtct ttgcgccgat taccccgatg
     3421 atgaagaatg catgcactgg atgctgaaat gcggaacgct acttcaacgc tgggatgccc
     3481 tcgcgacacg gctctcctcg tttgtcgttc gcaatccggg caatattgct atgcgatttg
     3541 ccctcgcggg agtactgttg agagcaggac gtcgagcgga tgcgcagcgg gaatatgact
     3601 ggttacgcgc catggtgccg acgttcgaag ggatggacga gttggcaaaa caattggccg
     3661 aatcagaacg cccgttggta ccgaaccatg ccgcatgagc catcgccggc gccccgcgat
     3721 ctggtgattc agatggctcg actgggtgat ctggtgcaaa cgctccccgc catcgaatcg
     3781 ttgcaggagg cttatccgaa acgcactctg gatgttctct gtgcggcacc gttgagcagc
     3841 ctccttgccg gagtgagtca tatcggccga gtcattccct gggacggggc acaatggcgg
     3901 gattgggcca ctgggtggcc agagaatccg acagtcacac tacaggtcat gcaacagtat
     3961 ctcgcatcgc tcggcgacac gacgtatgaa cgggtgtacc cgttaaatca acatgaacga
     4021 agccatctga tgacgcagct gttcctgagc cattccacgc gcgaacagga gcacgaccgc
     4081 atggaggcgc gcatccttcc atgggcacag catctccggc acgtcgccag ggaacggggg
     4141 gccaatcgcg tgcatctcgc tgatgcctgg tgcggcatgt gtggagtgct gccgcgcggc
     4201 caagcgccgt cgtgtgagcc gccgcccgtc gaactcccgc aagaccttgc cgaaatcggt
     4261 gagcgccagg ggcgctggat tgcggtggtc accggagcag gggaggcgga tcgttgtatt
     4321 cctcccgccg tgtggagcca atggattcgt gagttccttg cgcagaccca ggatgggcag
     4381 gtcgtgctga tcggaagcgg tacggagcgt gaaaccgcgc aggcgattct cgattcagtt
     4441 ccgacattgc tccacggtcg cgtctgggat gcgactggtc gtacgaccat ctcccagttg
     4501 atgcgtgtgc tgcagcagtg ccactgggtc attggcgctg atacggggcc gcttcatctt
     4561 ggcacgctgg cgggggctcg agctctcggc tggtattttt cgcgtgcccg aatgcacgaa
     4621 accggtccct atggtgaagg gcattgggtc tatcagcatg ccacgcaggc acagccccaa
     4681 cgttggccga tccaggaaag catcgcattg atgtgcggcg accagggtcg tcgtgcaacc
     4741 gattggaccc tatggaggag cggtatggat cgatggggag cgtatttcga tgacggatcc
     4801 ggaagcgcaa ctgtcgagtc ctcgcgtgcc gacatctggc gagcttgtgc accggccctt
     4861 tgcgaaccga tggcggcatg aatatgtttc aagacaatct gaatcgcctc gcttcacgcc
     4921 accgggatct cgcggcagcg gtgaaatctg cagccggcgg agtgctcacg atcgagccgg
     4981 cccgaaccgg cctaccgtcc gcgcgtcgtt ccggtcgctg gattcacagc gcttacgacc
     5041 cgcttcgtga agcgcagacc tgggcggata cccatgcagc ggcctgcaaa tctggtgaaa
     5101 tggtcgtcgt agccggggta gggctgctct atcatgtcga ggcgcttcga aagaagctgg
     5161 cgcctgatgt ctctctcgct gtcctgcttc ccaacctcga cgagtttcac gatgccctgg
     5221 tgactcgctc gctcgaaggc tggagtgagt ccattctctg gcttccagga acgccggctg
     5281 agattgccga gacattggca acgataggcc ggccgatacg ctgtctgtcc tatacgccgg
     5341 ctgctcttgc tgatgcagag ttccatcgcg ccgttgaaga cgcgttacgt caggggattg
     5401 cacgccaggc cggtggccaa ttgaccattg ccctcgtggg gcccatttac ggagggtcgc
     5461 tgccgattac gggctacgtc aaacgcgccc tggaaaccct cggtcataag gtacagctca
     5521 tcgatcacag catccatgcg gcgagttatg aggtgatggg cgcgctcaaa gacccgcgca
     5581 accgtcaatt gatgcaaggc cggatggccg atgtgttgag ccaatggacc ttagccacgc
     5641 tcgccgagtc gcctcctgat ctcgtgctct caatggcgca ggctcccatg acattgcagg
     5701 tattggagca cttgcgcaag aagaaattcc tgacggctat gtggttcgtt gagaactacc
     5761 ggcatctcac gtactggcag cagttggcgc cgggatacga gttctggttt gtctttcaac
     5821 agggggcatg
//
LOCUS       DAOQEW010000220         5790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5370_length_5790_cov_5.163208, whole genome shotgun sequence.
ACCESSION   DAOQEW010000220 DAOQEW010000000
VERSION     DAOQEW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_5790_cov_5.163208"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLS35_16980"
     CDS             <1..518
                     /locus_tag="PLS35_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNI20418.1"
                     /translation="GIPGVELSTGSLGHGLSVGAGMALSGKMNQLEYRVFVVLGDGEC
                     DEGSVWEAALFAPHRRLDNLVAIIDYNKLQGLDTTHEALELEPFAAKWRNFGWDVAEV
                     DGHDHEALEGALQQRSDRGKPRCVIAHTVKGKGVSFMENQVVWHYRWPRDNEVVLATE
                     ELMRSDVCATP"
     gene            503..1432
                     /locus_tag="PLS35_16985"
     CDS             503..1432
                     /locus_tag="PLS35_16985"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNI20419.1"
                     /translation="MRDTIIRVLTEASKVSRDIVLLTGDLGFGVLQNFSEQFPGRLIN
                     VGIAEQNMTGVAAGLSLEGKTVVTYSIGNFNTLRCLEHIRNDICYHQLHVIIVSVGCG
                     LSYGPLGFSHHSTEDMSIMRSIPNLIVVSPGCLWEAEQATTAALNSPGSYYLRVDKAS
                     VPSTERPGEQFVLGKARLFGDGRDCTLVTTGGMLRVVLAAQASLLHENIACRVISVPT
                     IKPFDSSVVLNSVRETKGMVTVEEHTIVGGLGSAVAEVLADHCAAPRFFRRIGLNDCF
                     ATVVGSQEYLREYYHIGESHIVQEVRRCIERSG"
     gene            1523..2281
                     /locus_tag="PLS35_16990"
     CDS             1523..2281
                     /locus_tag="PLS35_16990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI20420.1"
                     /translation="MDLGISGKRVLITGASRGIGRSCALDFAREHCEVTVVSRREPEL
                     TDLVEEMGGREKGHRFWACDLMRPGQPTRAFEALSAEEGPFEIIVHNLGGTLGIRDPL
                     SPMQDWFDVLHFNVGHAMEINALAIPQMQRRKWGRIVHVSSISAEHLRGSAPYGCAKA
                     YLNAYVKTVGRAFAADGIIMSALMPGAILAEGGYWDDIRKENPDKLADFLRSHHAIGR
                     LGSVEEISCFAVFMASKQVSFAAASLVPVDGGTM"
     gene            2312..3013
                     /locus_tag="PLS35_16995"
     CDS             2312..3013
                     /locus_tag="PLS35_16995"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNI20421.1"
                     /translation="MAYSVLKRGADLVLSVVAIVVLSPVFVLITLILRFSGEGEVFYR
                     QQRIGFQNKSFAMWKFVTMLKDSPNIGTRDISIEGDPRVLPVGRWLRKTKLNEIPQLF
                     NIVRGEMSFVGPRPLLEVSFQALEPTIQRRIFSVRPGLTGIASVVFRDENEIIAESKL
                     GPREGYYRVILPYKGRLEIWYQEHASFWVDLKIVFLTAWVVIFPKSRLVFRCFKEIPR
                     CEGLFILTGERGGER"
     gene            3247..4767
                     /locus_tag="PLS35_17000"
     CDS             3247..4767
                     /locus_tag="PLS35_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNI20422.1"
                     /translation="MSEVEREGMSVSLLSGRAYEIPAVLDDVVRIENFLSAHSGKRIV
                     VVQGLGFVGAVMALVCANALTEEYAVIGVDLPGPSSYWRIRSINDGVFPVVAADKKVE
                     EFHQKALAKGNLFATYDPYAYSKADVVIVDVNLDVQKDTTALGDLHKYDVDLTNFRKA
                     ITAIGEHCREDVLILVETTVPPGTCQLVVRPIIVECLTRRGLRVDRFRLGHSYERVMP
                     GPDYVDSIQNFYRVYSGVDEASAQATEGFLKTVVSTDQYPLTRLGSTNATEMAKVLEN
                     SFRAINIAFMVEWSRFAEEAGVNLYEVVNAIRMRPTHRNMMLPGIGVGGYCLTKDPLL
                     ASWARQYLIRGEGRLEESEKGVRINDKMPVYAYEFLKKSVAHGSLKGKRVLLLGVSYR
                     SDVGDTRYSPVERFFQQLVAEGCEVLLHDYYVSLWEETGRRVAADYVDFFANPLDIIA
                     ITTAHSGYRGSDRLQELILEQKPLLILDTVGLLSEVEIALLSRRHRVRVVGRGDLT"
     gene            4796..>5790
                     /locus_tag="PLS35_17005"
     CDS             4796..>5790
                     /locus_tag="PLS35_17005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNI20423.1"
                     /translation="MKKILLLGGAGFIGYNVVKYLAERGNCQIAIADNFFRANGKVDV
                     LLQRLIDTHPITLIKGDFTNPEAYRWLDSGYDEVYMLASVVGVDYVNKIPHEIIRINT
                     SLILNTLEWLRGSGCGKVLFTSTSECYAGAVEAFHYPIPTAEDVPLTIQDIAHPRFTY
                     AVTKMLGESGFLNYSRLLGFECSIVRYHNVYGPRMGFKHVIPHLAQRFADKEDPFRIF
                     GHNQTRSFNYIDDAVRGTVLAMEKGRNGEIYHIGDMDEITIEDLVRYVGELAGYAGTY
                     EYAPTYPGSVARRCPDIAKARRDLGYSPEVPWREGVRRTMNWYWEYLRSGVELHES"
BASE COUNT         1269 a         1319 c         1836 g         1366 t
ORIGIN      
        1 agggtatccc cggcgttgaa ttgtcgaccg gatcgctagg gcacggcctg tctgtgggcg
       61 ccgggatggc gcttagtgga aagatgaacc aactggagta ccgtgtgttt gtcgtgctgg
      121 gtgacggcga atgcgacgaa gggtctgttt gggaggcagc gttgttcgcg ccacatcggc
      181 gtctggataa tctggttgcg attattgact acaacaaact gcagggcctg gatacgacgc
      241 acgaagctct ggagctcgaa ccatttgcgg ccaagtggag gaattttggg tgggatgtgg
      301 cggaggtcga cgggcacgac cacgaggcgc tcgaaggcgc attgcaacag cggtcagatc
      361 gtggcaagcc acgctgcgtg attgcacaca cggtcaaagg gaaaggagtt tcctttatgg
      421 agaatcaagt cgtttggcat taccggtggc cccgggataa cgaggtggtt ttagcgaccg
      481 aggaacttat gcggagcgac gtatgcgcga caccataatt cgagtgttga cagaagcatc
      541 caaagttagt cgggacatag ttctgctgac gggagatctt gggttcggtg tcctccaaaa
      601 tttttcggag cagtttccag ggcggctaat caatgtagga atcgcggaac agaacatgac
      661 cggtgtggcg gctggtctgt cgcttgaagg taagacggtg gttacttact cgattggcaa
      721 tttcaatacg ctacgctgcc tagaacacat ccgcaacgac atttgttatc atcagttgca
      781 tgtgatcata gtgtcggtcg gctgcgggct cagctacgga cctctcgggt tttcgcatca
      841 ttcgactgaa gatatgagca tcatgcgaag catcccgaat ctcatcgtgg tcagcccggg
      901 ctgtttatgg gaagcggagc aggccacaac tgctgcgctg aatagtccgg gttcgtacta
      961 cctcagggtc gacaaggcgt ctgttcctag tacggagcgg ccgggagaac aattcgttct
     1021 tggtaaagcg cgactattcg gagatggccg agactgcacg ttggtaacaa ccgggggaat
     1081 gctgcgcgtg gtattggcag cgcaggcttc actgttgcac gagaacattg cgtgccgagt
     1141 tattagcgtc ccaacgatta agccgttcga tagtagcgtg gtgttgaatt ccgttaggga
     1201 gaccaaaggt atggttacgg tggaagaaca taccattgtg gggggcctgg gtagcgctgt
     1261 ggcagaagtg ctcgcggatc actgtgccgc gccgaggttt tttaggcgta tcggtctcaa
     1321 cgattgtttt gcaacagttg tgggatccca ggagtatctt agagagtact atcacatcgg
     1381 ggaaagccat attgttcagg aggtgcggcg atgtatcgag cgcagcggct gataatgacg
     1441 aaggatgaga aagtcccccg agtaggtgag cgagcggagg aagctattta ccttgtcgta
     1501 acgtattttt gaggcgctaa caatggactt gggtatatct gggaaaaggg ttctgatcac
     1561 cggtgctagc cggggaattg gccgttcatg tgctttggat ttcgctcggg agcactgcga
     1621 ggtgacggta gtttctcgca gggagccgga gctaacagat ctcgtcgaag agatgggcgg
     1681 cagagaaaag gggcatcgtt tctgggcgtg cgatctaatg cgacccggtc agccaacgcg
     1741 tgccttcgaa gccttaagtg cagaggaagg tccttttgag attatcgtcc acaatctggg
     1801 tgggactctt ggtattcgcg atccgctgtc accgatgcaa gactggtttg atgtcttgca
     1861 tttcaatgtg ggacacgcga tggaaatcaa cgcgttggcg attccgcaaa tgcagcggag
     1921 aaaatggggg cgaattgttc acgtttcctc gatttcagcg gagcacctgc ggggctccgc
     1981 tccctatggc tgcgcaaagg catatctgaa cgcttatgtg aagaccgttg ggagggcatt
     2041 tgcggcggat gggattatta tgtcggcttt gatgccaggc gccatcctcg ccgaaggagg
     2101 ctactgggac gacattcgaa aggagaaccc agataagctc gcggacttct tgcgaagcca
     2161 tcatgccatt ggtcggctcg gaagtgtcga ggaaatttcg tgtttcgccg tgtttatggc
     2221 ctcgaagcag gtcagcttcg cggcggctag tttggtgccg gtggatggag gcaccatgta
     2281 agtatacgac agcaagttga cgggataatc tatggcttat tcggtattga aacggggcgc
     2341 ggatctggtt ttgagtgtgg tcgccattgt ggtgctctct ccagtgttcg ttttgatcac
     2401 gttgatactc aggttttcag gagaagggga agtgttttac cggcagcagc gtattggctt
     2461 ccagaacaag tcatttgcca tgtggaagtt tgtaactatg ctgaaggata gcccaaatat
     2521 tggtacccgt gatattagca ttgaaggaga cccgcgagtc ttgcccgttg ggcgatggct
     2581 gaggaagacg aagcttaacg agattccgca gctgtttaac atcgttagag gcgagatgag
     2641 cttcgttggc ccgcgtcccc ttttagaggt gagctttcag gcgttggaac cgacgattca
     2701 gagacggata ttttcggtcc gaccggggct gacggggatc gcatcggtag tgttccgaga
     2761 tgagaacgag attattgcag agtcgaagct gggtccgcgt gagggctact acagagttat
     2821 cttgccgtac aaggggcgtt tagagatttg gtatcaagag cacgcttctt tttgggtgga
     2881 cctgaagatc gtctttctaa ccgcttgggt ggtgatcttc ccgaagagtc gacttgtatt
     2941 tcggtgtttt aaggaaatcc cgcgctgtga aggtttgttc attcttacag gcgaacgcgg
     3001 gggggaacgc taagcgcgta ctgtagcccg ccaaatggcg ttcatcgtgt tctcggaagg
     3061 aattcggagc taggaaagta ggtaaggggt tcaaaagtac gataaaacag gaacggggta
     3121 gaggggtgtc tcgcacgctc gccggcggtt ggtggcttgt tgtgcaggag aatctggaag
     3181 cgcagagggc ggtatttttc gtaggttttg tagtagcgcc tgcgtaggct ttggagacca
     3241 agtaaaatga gtgaggtaga gagggaaggt atgtcggtaa gcttgctttc agggcgagct
     3301 tacgaaattc cagcggtgtt ggatgacgta gtacggatag agaatttcct atcggcgcat
     3361 agcggaaagc ggattgttgt cgtccaagga cttggattcg taggtgcggt gatggcttta
     3421 gtctgcgcga acgccttgac cgaagagtat gcggttatcg gggtcgatct gcccggtcct
     3481 tctagttact ggagaattcg ttcgatcaat gacggggttt tcccggtggt cgcggcagac
     3541 aagaaggtgg aggagttcca tcaaaaggct ctggcgaaag gtaacctttt cgctacttac
     3601 gatccgtatg cttattcaaa agcggacgta gtcattgtgg atgttaatct cgacgtacag
     3661 aaggatacca cggctctcgg cgatcttcac aaatacgacg tggatctcac caacttccgc
     3721 aaggcgatca cggcaattgg cgagcattgc cgagaggacg tgcttattct ggtcgagaca
     3781 actgtccccc cagggacttg ccagctggtt gtccggccga tcattgttga gtgcttgact
     3841 aggcgcgggt tacgggtcga ccgtttccgg ctaggtcact cctacgagcg cgtgatgccg
     3901 ggtcccgatt atgtggattc aattcaaaac ttctatcgcg tttactcggg agttgatgag
     3961 gcgagtgcac aggcgacgga gggatttttg aaaacggttg tcagcacgga tcagtacccg
     4021 ttgacacgtc ttggaagcac caacgcgacg gagatggcta aagtgctcga gaactcattc
     4081 cgagcgatta acatcgcttt catggtggaa tggtcgagat ttgccgagga ggcgggagtc
     4141 aatctctatg aggtggtcaa cgccattcgg atgcggccca cacatcggaa catgatgctg
     4201 cctgggatag gggtcggtgg ctactgtttg acgaaagatc cactgttggc ttcctgggcc
     4261 cgccagtatc tcattcgggg ggagggcagg ctcgaggaga gcgaaaaggg tgtgcgtatc
     4321 aacgacaaga tgccggtata tgcctatgag ttcctcaaga agtcagttgc gcacggcagc
     4381 ctgaagggta agcgcgtact gttgctgggt gtttcctacc gcagcgatgt cggcgatacc
     4441 cgctactcgc cggtggagcg gttctttcaa cagcttgtgg ccgaagggtg cgaagtgctg
     4501 ctccacgact actacgtaag cctgtgggaa gagacaggac ggcgggttgc ggcggactat
     4561 gtggattttt tcgccaaccc actcgacatc atcgcgatca caacggcgca ttccggatac
     4621 cggggatcgg atcggttgca ggagttgatc ctggagcaga aaccgctgct catccttgac
     4681 actgtcgggc tgctctccga ggtggagatt gcgctgttat cgcggagaca tcgtgtgcga
     4741 gtggtcggca gaggcgacct aacgtgaaac cctttctgac aaattgagca tcccgatgaa
     4801 aaaaatacta ctccttggcg gcgctgggtt tatcggctac aacgtggtga agtacctcgc
     4861 cgagcgcggc aactgccaga ttgccattgc cgacaacttc tttcgcgcga atgggaaggt
     4921 ggacgttctg ctgcaacgac tgatcgacac gcacccaatc acgctgataa aaggggattt
     4981 cacgaatccc gaagcttacc ggtggctgga cagcggttac gacgaggtct acatgctggc
     5041 ctcagtggtc ggcgtcgatt acgtcaacaa gattccgcac gagatcatac ggatcaatac
     5101 ctcgctgatt ctcaacacgc tggaatggct gcggggatcc gggtgtggca aggtgctgtt
     5161 cacctcaacg agcgaatgct acgccggggc ggtcgaggcc tttcactatc ctatccccac
     5221 ggcggaagac gttccactga cgattcagga catcgcgcac ccgcgcttta cctacgcggt
     5281 gacgaagatg cttggggagt cgggtttcct taactattct cgcctcctcg gtttcgaatg
     5341 cagcatcgtt cgctaccaca acgtgtacgg cccacggatg ggattcaaac acgtgattcc
     5401 gcacttggcg cagcgatttg ccgacaagga ggaccctttc cggattttcg gccacaatca
     5461 gacgcgctca ttcaactaca tcgacgatgc ggttagggga acggtcctgg cgatggagaa
     5521 gggccgtaac ggagagattt atcacatcgg ggatatggac gagatcacga ttgaagatct
     5581 cgttcggtat gtgggtgaat tggctggata tgccggtaca tacgagtacg cgcctacgta
     5641 tccgggctca gtggcgcggc gctgccccga catcgcgaag gcgcggcggg atttgggcta
     5701 ttcgccggaa gtgccgtgga gggaaggagt taggcggacg atgaactggt attgggagta
     5761 cctgcgcagc ggcgtcgagt tgcacgagtc
//
LOCUS       DAOQEW010000221         5722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5474_length_5722_cov_7.594142, whole genome shotgun sequence.
ACCESSION   DAOQEW010000221 DAOQEW010000000
VERSION     DAOQEW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_5722_cov_7.594142"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLS35_17010"
     CDS             <1..319
                     /locus_tag="PLS35_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNI20424.1"
                     /translation="DDSSNWSAFMAGALIGAGIALLLAPQSGNELRSTLRDYASRAKD
                     ELDEATEQGRAAWDSAMERGQEYVEGGKQTLRKAGRAAREFVDETMQTAQNTAEEATS
                     RRG"
     gene            380..688
                     /locus_tag="PLS35_17015"
     CDS             380..688
                     /locus_tag="PLS35_17015"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNI20425.1"
                     /translation="MMKLLTHLMFAFAMMTLVACQSTTGKTAGQTIDDASITTAVQAK
                     LSQDRLSNFSRIDVDTERGVVTLNGVVKSAEQKMRVVELARGVAGVRTVNNNLQIQAP
                     "
     gene            765..2234
                     /locus_tag="PLS35_17020"
     CDS             765..2234
                     /locus_tag="PLS35_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNI20426.1"
                     /translation="MTRHVSHILPVLFSALLVGACSHSIPKSPSKPVPPPLTSAVPTF
                     PAQTKEDSRAPVSQVPVQIKTDLTPIQTAIRKAIPERITETGHPLGQEFRWTFVRVGE
                     PDVHIQDGLVAIHAEYKGDIESRGSSRACRLDPLYTSIDATGRLTMIQNGDALSFGFE
                     PAQTAVTTKPDSDARCNMFNVPISEQLPDLFGMSEIKTALAEAVHPKEFGIPLQRIWD
                     DLGGPLSIPIASLNTRACMYGNPREMILGGQKGTTQETVISGTAKEMPFITYEATCTE
                     ATPTVALVNSGSIPPEGKPYTLVARIPLSYQQISHQLQSKLFHQSVFLDTASDTAVIE
                     RVSASDANGRALVAIDMSGDLKGTIYYWGTPRLEEGGRSISVPDLQMANESKAAIDSI
                     RIGYWQLVDRELNGKLRQAMTVDISPQVDRLKQTVTGTHRSGGTTMDILITRQQPDHV
                     RSSAQGITATIMLEGTANATGQVTLEEQSPRALLRQDVR"
     gene            2273..2689
                     /locus_tag="PLS35_17025"
     CDS             2273..2689
                     /locus_tag="PLS35_17025"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI20427.1"
                     /translation="MEQRPTLHETADIIKIMKNDHRHILALFQVYLGTESDSRQSIVD
                     DILQRLDDHFDWEERLFEEDSRLQEHATPVIRRVLLDHEEVKAMIHELRHAETDDDES
                     MDQFFEDMMQTVRVHFHGEERDLIPLLDAMTTAPRG"
     gene            2769..3872
                     /locus_tag="PLS35_17030"
     CDS             2769..3872
                     /locus_tag="PLS35_17030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNI20428.1"
                     /translation="MSHSPLRVAVVGLGHISQMAVLPAFSHTGSAATLTALVSDDPVK
                     RRVLAKRYGVTHTYPYKDFDACLRSGEIDAIYIALPNSLHCEYAVRAAKAGIHILCEK
                     PMAVTAAECRRMIQAAERHRVKLMIAYRLHFEACNLFTIDLVQSGRLGDPRLFHSVFT
                     QQVRAGDIRLRQQLGGGPLYDIGVYCINAARYLFRDEPTEVTALAGRGPDHRFQSVEE
                     SAGAIMRFSRDRLATFTCSFGAADAATYEVVGTKGKVRVEPAYEYVGGLKASVTIGGR
                     TSVRTFTPGDQFAPQLIYFARCVTHDRQPEPNGTEGLLDVQIIEALHRSAQRGQSITL
                     RLPAKRLRPDLTLRMHRPPVSKPQGVHTESPTL"
     gene            4006..4356
                     /locus_tag="PLS35_17035"
     CDS             4006..4356
                     /locus_tag="PLS35_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20429.1"
                     /translation="MEQDMKHMADRAKQAGSSIQDAIHDTATSAEHMAKRAGDAVESA
                     ADHVRDRLPKDSAAGEAADTVVRKVKETSVYLQEQGIGGVVEDLEILIRRYPLQTLLL
                     GLGCGYLLSRARTD"
     gene            4375..4782
                     /locus_tag="PLS35_17040"
     CDS             4375..4782
                     /locus_tag="PLS35_17040"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNI20430.1"
                     /translation="MENPHPISIASLVTGLVEDLRRLFAQEVQLARHEMQHELGKVVK
                     SAMHAGIAMMLAFLAVLFLLMTLIHLLHFYSGLPLWACYGIVALVAAAAGAAFFARLI
                     AIGTTLRLWPFRTFYSLKEDAKWIKEQLLSTKT"
     gene            4803..5462
                     /locus_tag="PLS35_17045"
     CDS             4803..5462
                     /locus_tag="PLS35_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20431.1"
                     /translation="MDIAEKLELLEQRIKDTAEGATMKFSRLLDEGSQSVTQMVDKTK
                     AALDPIHKVDDYPWLMLGGAICAGYAIGLLEARTRAQRGVYPYYPPGAHASQVMPESE
                     TEGKTASRRAEGIYDYYPDPTHRARRTEQRSHSTIWETMSRELEIDTEQAKAALMQAG
                     RTLMVEFANKLIPEIARSLGVTLTSQGKQSRPSYDQASYSETEGRGNARSSAGSASQF
                     R"
     gene            5478..>5722
                     /locus_tag="PLS35_17050"
     CDS             5478..>5722
                     /locus_tag="PLS35_17050"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNI20432.1"
                     /translation="MSASLTSASPRWNPWKLGGLGWKELGQRLWTDSQKDEILGRAAQ
                     LAYYFLLALFPALLFLTALLGLFPLEQVLPELMTYLRT"
BASE COUNT         1287 a         1673 c         1606 g         1156 t
ORIGIN      
        1 ggatgattct tccaactggt cggcattcat ggcgggcgca ttgatcggcg cgggaatcgc
       61 gttgctcctt gctcctcaat caggcaatga acttcgctcg actctgcgtg actatgcctc
      121 cagggcgaag gacgaactgg atgaagccac tgaacaggga cgcgcagcct gggactccgc
      181 catggaacga gggcaggaat acgtcgaggg tggcaaacaa acgttacgaa aggcagggcg
      241 agccgctcgt gagttcgtgg atgagacgat gcagactgcc cagaacacgg cggaagaagc
      301 gacatcacgg cgaggctgag aagagccgcg ctagagagtc agtgatgctg tcacactacg
      361 ttgaaaagga tgcgctatca tgatgaaact actcacgcac ctcatgttcg cctttgccat
      421 gatgaccctg gtggcctgcc agtccaccac cggcaagact gccggtcaga ccatcgacga
      481 tgcctccatc accacagcgg ttcaggccaa actgtcacaa gaccggctgt cgaacttttc
      541 gcgaatcgat gtcgacacgg agcgcggcgt cgtgaccctg aacggggtgg tcaaatccgc
      601 cgaacaaaaa atgcgggtcg ttgaactggc tcgcggggtg gccggtgtgc gcaccgtgaa
      661 caacaactta caaattcaag cgccgtaaga aaccaggatg agcatcttct gggggaggcg
      721 gcgatcgcac gatcgctgct gtgatgaggt ctaggaggct gattatgacg cgccatgtct
      781 cacatatact gcctgtgctc ttttctgccc tgttggtggg agcctgcagc cattccatac
      841 ccaaatcgcc atccaagccg gtcccgcctc ctctgaccag tgccgtgccc acattcccag
      901 cgcagaccaa agaggattcc cgggcgcctg tgtcccaggt gcccgttcaa atcaaaacag
      961 acctgacccc catccagacc gcgatccgca aggccattcc cgaaaggatc acggagaccg
     1021 gccaccccct ggggcaggag ttcaggtgga cttttgtgag ggttggtgag cctgatgttc
     1081 acattcaaga tgggctggtc gcgatccatg ccgaatataa aggtgatatc gaatcgcgtg
     1141 gcagttcacg tgcctgcagg ctcgacccgt tatacacctc aatcgacgcc acaggccggt
     1201 tgacgatgat tcagaacggc gatgctctat ctttcggctt cgaaccggca cagacagccg
     1261 tcaccaccaa acctgatagc gatgcgcgct gcaatatgtt caatgtcccg atttccgaac
     1321 agcttcccga tctgttcggc atgtcggaga ttaaaacggc cttggctgaa gcggtacatc
     1381 ccaaggagtt cgggattcca ctgcaacgaa tctgggatga tttgggcggc ccattatcga
     1441 tccccattgc gtctctcaac acccgggctt gtatgtatgg caatcctcgg gaaatgattc
     1501 tcggcggcca gaaagggacc acgcaggaga cagtcatttc gggaacagcg aaagagatgc
     1561 cgttcattac ctatgaagcg acctgtacgg aagccacacc gacagtggcc ctggtcaatt
     1621 caggatccat tccacccgaa ggaaagcctt atacgctcgt agcgcggata ccgctgtctt
     1681 atcagcaaat ctcgcatcag ttgcagagta aactctttca ccaatcggtg tttttggata
     1741 ccgcttccga taccgccgtc atcgaacggg tctcggcctc ggatgccaac ggccgcgcgc
     1801 tggtggcgat cgacatgagt ggcgatctca aaggcacgat ctattactgg gggactcctc
     1861 gtctcgaaga agggggacgc agtatttctg ttccggatct ccaaatggcc aatgagtcca
     1921 aagccgcgat cgattccatc cgcatcggct actggcagct ggtcgaccgt gaattgaacg
     1981 gcaagttgcg gcaagcgatg accgtcgaca tctctcctca agtggatcgc ttgaagcaga
     2041 cggtgaccgg tacccatcga tcgggcggca ccacaatgga cattctcatt acgcgccagc
     2101 agccggatca tgtccgatcc tctgctcagg gcatcacggc caccatcatg ctggaaggca
     2161 cggccaacgc gaccggacag gtcacgcttg aagaacagag tccccgcgct ctgcttcgac
     2221 aggacgtccg gtaacggccg gcgctcacga tatgccacgc ggaggcggat ctatggaaca
     2281 gagaccgacg ctgcacgaga ccgccgacat catcaaaatt atgaagaatg accaccgcca
     2341 tatactggcc ctattccagg tatatctcgg tacggagtcg gattcccgac agtccattgt
     2401 cgacgatatt cttcagcggc tggatgatca cttcgactgg gaagaaagat tgttcgagga
     2461 agacagccgc ctccaggaac acgccactcc agtgatccgg cgcgttctgc ttgaccatga
     2521 ggaagtcaaa gccatgatcc atgaattgcg ccacgcggag acggacgatg atgaatccat
     2581 ggatcagttc tttgaagata tgatgcagac tgtgcgtgtc cattttcatg gcgaagagcg
     2641 tgatctcatt cctctgctcg atgccatgac taccgcaccg cgtgggtgac ccggccttgc
     2701 catgcgaagc gtttggggcc gactctccgc cggcacattt tttcatgttc acaaaggagt
     2761 agtaggaggt gagtcattca ccgttgcgtg tggcagtcgt aggactcggc cacatttccc
     2821 agatggcggt cctaccggct ttttctcata cgggatctgc cgccaccctt accgcactcg
     2881 tctcggacga tccggttaag cgtcgtgtcc tcgccaaacg ctacggcgtg acccatacct
     2941 acccgtacaa agacttcgac gcatgtctgc gtagcggcga gatcgacgcg atctatatcg
     3001 ctctccccaa cagcctgcat tgcgaatatg ccgtacgagc cgcgaaagcc ggcatccata
     3061 tcctttgcga aaaaccgatg gccgtcacgg cggctgaatg ccgccgcatg atacaggctg
     3121 cggaacggca tcgcgttaaa ctcatgattg cctaccgcct gcattttgaa gcctgcaacc
     3181 tcttcacaat cgacctcgta cagtcgggac ggttgggcga cccgcgcctt tttcattcgg
     3241 tgttcacaca acaggtccgg gcaggcgata tccgcttgcg gcaacagctc ggcggcggtc
     3301 ccctgtatga cattggagtc tactgtatca acgcggcccg gtatctattt cgggatgagc
     3361 ccacagaagt caccgccctc gccggtcgag gaccggatca ccggtttcaa tccgtcgaag
     3421 aatcggccgg agcgatcatg cgattttctc gagatcgcct cgcgaccttc acctgtagtt
     3481 tcggcgccgc cgacgccgcg acctatgagg tggtcggcac caagggaaaa gtacgcgtcg
     3541 agccggcgta tgagtacgtc ggtggattga aagcctccgt cacgatcgga gggcgcacga
     3601 gcgtacgtac gtttacaccg ggcgatcaat tcgcgccaca actgatctat tttgcgcgct
     3661 gtgtcacaca tgatcgacag ccggaaccga atggaacgga aggtctcctc gacgtccaga
     3721 ttattgaagc gctccaccgg tccgcccagc gcggacaatc gatcacccta cggctgcctg
     3781 ccaaacgcct tcgtcccgat ctcacgcttc ggatgcaccg cccgcctgtg tcaaaacctc
     3841 agggtgtgca tacggagtcg ccgactcttt aatgtcggtg cgatcgctgg cgcgagactc
     3901 ctggctgact agggctctgc ccagttcaaa catcatggtc acgccgctat tctgggcaag
     3961 tatctgctca acatgcaccc tatacacgag tgaggaggag ggccgatgga acaggatatg
     4021 aagcatatgg cagaccgggc gaaacaggcc ggctcgtcaa tacaagatgc catccacgac
     4081 accgccactt cggcagaaca catggcgaaa cgcgccggtg acgcagtgga atcggctgcg
     4141 gaccacgtcc gtgaccgtct tcccaaagac agcgctgcag gcgaggcggc ggacacagtc
     4201 gtgcgcaagg tcaaagaaac ctctgtctac ttacaggaac agggcatcgg cggggtcgtc
     4261 gaggaccttg aaattctcat tcgacgttac ccgctccaaa cactgctgct cggcctggga
     4321 tgcggatatc tgctctcccg cgctcgaacc gattaaccgg aagaggacgg ctatatggaa
     4381 aatccccatc cgatttcgat cgcctcgctg gtcacagggt tagtggagga tcttcggcga
     4441 ttgtttgccc aggaggtcca actggcccgg catgaaatgc agcacgagtt ggggaaggtc
     4501 gtcaaaagcg ccatgcacgc cggcatcgcc atgatgctgg ccttcctggc tgtattattt
     4561 ctcctgatga cgttgatcca cctgttgcac ttttacagcg gcctcccact gtgggcctgt
     4621 tatggaatcg tggcactcgt tgcggctgcc gcaggcgcgg ccttctttgc tcgtctgatc
     4681 gccatcggca ccactcttcg gctctggccg tttcgcacct tttactcatt aaaggaagat
     4741 gccaaatgga tcaaagaaca gctgctatcg accaagacat aaaagatatt gtggagacga
     4801 gaatggatat cgccgaaaag ctcgagctgt tggaacaacg gatcaaagac acggcggagg
     4861 gagccacgat gaaattttcg cgcttactcg acgaaggatc ccagagcgtc acgcagatgg
     4921 tggacaaaac caaagccgcc ttggacccca tccataaggt cgatgactac ccatggctga
     4981 tgctgggcgg agcgatttgc gccggatacg cgatcggtct gttggaagcc cgtacgcgtg
     5041 cacaacgcgg ggtgtacccc tattatccgc cgggcgctca cgcatcacag gtcatgccgg
     5101 agtcggagac ggaaggcaaa accgcttctc gtcgagccga aggcatttac gactactatc
     5161 ccgatcccac ccacagggcc cgcagaacgg aacagcggag tcactccacc atctgggaaa
     5221 ccatgagccg tgaattggag atcgatacgg agcaggccaa ggccgcatta atgcaagccg
     5281 ggcgtacgct catggtcgaa ttcgcaaaca agctgattcc cgaaatcgcc cggtctctcg
     5341 gcgtgacgct gacctcccaa gggaagcagt cccgaccgtc gtacgaccag gcttcgtatt
     5401 cagagactga aggacgcggc aacgccaggt cttcggccgg atcggcatca cagttccgat
     5461 gaagtagagg agtcagggtg tcggcctcgc tgaccagtgc atccccacga tggaatccct
     5521 ggaaactcgg cggactggga tggaaagagc ttgggcaacg gctttggacc gacagccaga
     5581 aggatgaaat cctcggccgt gcggctcaat tggcgtatta ctttctcctg gctctttttc
     5641 ccgcgctgct gttcctgact gcattgctgg gcctgtttcc gttggagcag gtgctgccgg
     5701 agttgatgac ctatctccgg ac
//
LOCUS       DAOQEW010000222         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5475_length_5721_cov_9.449700, whole genome shotgun sequence.
ACCESSION   DAOQEW010000222 DAOQEW010000000
VERSION     DAOQEW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5475_length_5721_cov_9.449700"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..697
                     /locus_tag="PLS35_17055"
     CDS             182..697
                     /locus_tag="PLS35_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20433.1"
                     /translation="MFTSAARRSPWIGRLLLGFVLILGVSCSGKTIQYKDDHDRILRI
                     DQAVESLRKSYVERDRSALEALLLPSGGLDQVQRDIHSDFDTFRDIQLEFSIERILIE
                     GDNIDVFVHWQGQWKRDPGDVGTRQRGHGRLAWVGTQSILLREAQGDLPFGMGTRASL
                     NEDPARSQPKP"
     gene            700..2376
                     /gene="nadB"
                     /locus_tag="PLS35_17060"
     CDS             700..2376
                     /gene="nadB"
                     /locus_tag="PLS35_17060"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNI20434.1"
                     /translation="MSARSASPKIETDFLVVGSGVAGLRAAIELSRAGRVLMLTKGHP
                     LESNSMYAQGGVAVALSEEDDVGSHLTDTLKAGHGLCRREAVRVLVEEGPERIQELIA
                     WGAKFDKIGKRFAFTREAAHSRSRILRARGDATGNEMVRALMTYAARQPRIQRLDRRF
                     TVDLAVVDGRCCGAIVLNEMSGERTVLPASAVVLSTGGAGQVYARTTNPGNATGDGMA
                     MALRAGALLMDMEFVQFHPTSLYLPSSPPFLLSEAIRGEGGQLRNIKGELFMHRYHPD
                     GALAPRDVVSRAIWSEMAATRARHVYLDVTHLGAAFVKRRFPTIYATCLRYDIDMTEE
                     WIPVSPSAHYMMGGVWTDVHGATTLPGLYAAGEVACSGVHGANRLASNSLLEGLVFGM
                     RAAQSAVAHAGHFSESVQLPSLEELEQGRPTELDDPEKLRSSLRRLMWGKVGLVRSGD
                     SLISASAQLVRWMRLLGKPFSSRAALEVKNMVQVARCITDAALWRENSVGAHYRSDFP
                     EAKRQGWRNHSQLLLGGGQVNGMPSNERDLVLATEAPVGRKPGKKTSGRS"
     gene            complement(2282..2947)
                     /locus_tag="PLS35_17065"
     CDS             complement(2282..2947)
                     /locus_tag="PLS35_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNI20435.1"
                     /translation="MARFKRTTPLLLLYATLLLTHAPVQAEVYQYIDANGTISLTNVP
                     NDPRYKRVMSELPRSRSVISDRELDPVIARHSRAHRLHPALIRAVIKTESDFDPLAVS
                     HAGAVGLMQLMPQTAMRFDVRDSYNPDENIGGGTKYLRQLLDRFNGNLPLALAAYNAG
                     EHAVERYQGLPPIPETRQYVVKVLGYYRTFLMNARTSSSRAYVPQAPRSQGRGLAHST
                     ASR"
     gene            complement(3098..3952)
                     /locus_tag="PLS35_17070"
     CDS             complement(3098..3952)
                     /locus_tag="PLS35_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNI20436.1"
                     /translation="MPKEQAVVSVFDHGFLYGDGVYETLRSYGSRIFMRDQHLARLRR
                     SAEAIGLTIPIPDAQWPDLLHEAMRRNEVGNDRVDAYLRITISRGEGEIGLDPALCPR
                     PTVVILTKVLHPSPPALSRDGVTLTVANTRRNLPEALSPHIKSTNFLNNILAKRESIA
                     AGTFDSLFLNWREELTECTISNLFFISNGTVHTPSLDCGILDGITRAIVMTLAEEEGL
                     PVQEGHYHLADLRQADECFLTNTSMEVMPVSRVDAWPIGDGRPGPLTRRLQARFAASR
                     TRFLAPAP"
     gene            complement(3979..5463)
                     /locus_tag="PLS35_17075"
     CDS             complement(3979..5463)
                     /locus_tag="PLS35_17075"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNI20437.1"
                     /translation="MTAVSHQAFLNGPPQPLVVVRPLPNADAFELYQRLGITDGPSFL
                     LESGNGTNATARYSFFGNHPYLTLTGRAQDYSMKADGHITTAHGYAFDAFRQAFADST
                     IPQPDGLPPFYGGAVGYLSYDLVRSFESLPTLASDDLGLPDLHMAFFDTVAAVDHHTD
                     QLYLMYCPPLSRFRAEPREKLLREGSDRLAELEARLTSISRPHPAPTGPIQTSFVPSQ
                     SRDAYTARVRRCQDYIAAGDIYQANLSHRFTLNLDASWSAADRDRYAGILYSRLRRVN
                     PSPFAGLVRFPELTLVSSSPERLVRLTASQASTRPIAGTRPRGNGESHDVTMRAELLA
                     SPKERAEHVMLVDLERNDLGKVCQYGSVRVDEFMTIEQYSHVSHLVSEVVGTLRPGTS
                     PFDLVRAVFPGGTITGVPKLRCMEIIDELEPVRRGLYTGALGYFSWSGDLDLNILIRT
                     LVLTKDRGYLQVGAGIVADSDPDREYDETLAKAGAFLHILEADR"
BASE COUNT         1152 a         1572 c         1833 g         1164 t
ORIGIN      
        1 actcgccata cattcaatct ccaactgcta ggcgcgggag tctagtgggt ttcagttcaa
       61 aaagcaagtc aagattctga cgtgttgtct gctgtcgaat cattcgaagg gatcggaagt
      121 cagtcacgta agggccaggc cgttgcttga cctctttccg ccaagattcc tatactgcgc
      181 catgtttact tctgctgcgc gccgttctcc ctggattggt cgacttctcc tcggtttcgt
      241 gctgattctg ggcgtgagtt gctccggaaa aaccattcag tataaagacg atcacgaccg
      301 tattctccgg atcgatcagg ccgtggagtc actcaggaaa tcctacgtgg agcgggaccg
      361 gagcgccttg gaggcgttgc ttctcccgtc tgggggcctg gaccaggtgc aacgggatat
      421 ccatagcgat tttgacacct ttcgcgatat tcaattggag ttttccatcg agcgcatttt
      481 gattgaaggc gacaacatcg atgtgtttgt ccattggcag ggccagtgga aacgggatcc
      541 cggcgatgtc ggcacgcgcc aacgaggcca cggccgattg gcctgggtcg ggacacaatc
      601 gattctgctt cgagaggctc aaggcgatct gccgttcggc atggggacac gagccagcct
      661 caatgaagat cccgctcgca gccaacccaa accctagcca tgtcagcccg cagtgcatcg
      721 ccaaaaattg aaacagattt tctcgtcgtc gggagcgggg tcgcgggttt gcgcgcggcc
      781 attgaattga gccgagcggg acgtgtgttg atgctgacca aggggcatcc cctggaaagc
      841 aattccatgt atgcccaggg cggtgtggcc gtcgcactca gcgaagaaga cgatgtcgga
      901 agccatctca ccgatacgtt aaaagccggg catggcctct gtcgccgcga agccgtgcgg
      961 gtgttggtcg aagaagggcc ggagcgaatt caggagctca ttgcctgggg ggccaagttc
     1021 gacaaaatcg gcaaacggtt tgcgtttacc cgcgaagccg cgcacagccg aagccgcatc
     1081 ctccgcgcac ggggggatgc caccggcaac gagatggtgc gcgcgctcat gacctatgca
     1141 gccaggcagc cccgcattca gcgattggat cgacgattta cggtcgacct ggcggtggtc
     1201 gacggacgtt gttgcggcgc catcgtgttg aacgaaatgt cgggggagcg caccgtgctg
     1261 ccggccagcg cggtggtctt gtcgaccggc ggagcgggcc aggtgtacgc gcgtacgacg
     1321 aatccgggca acgcgacggg cgacggcatg gcaatggcgc tgcgggccgg cgcgttgctg
     1381 atggacatgg agttcgtgca attccatccc acctcgctgt acctgccgtc gagtcctcct
     1441 ttcctgctat cggaagccat tcgcggagaa ggcgggcagc ttcggaacat caagggcgag
     1501 ttattcatgc accggtatca tccggacggc gcactggcgc cgcgcgatgt ggtctcccgg
     1561 gcaatctggt cggaaatggc cgccacccgt gcgcgtcatg tctatttgga tgtcacacac
     1621 cttggcgcag cctttgtgaa gcgccggttt cccacgatct acgcgacctg cctgcggtat
     1681 gacatcgata tgacggagga gtggattcct gtctccccca gcgcgcatta catgatgggt
     1741 ggcgtgtgga ccgatgtgca tggcgcaacg acgttgcctg ggttgtatgc ggcgggagaa
     1801 gtggcctgca gtggcgtgca tggggccaat cgattagcca gcaattcatt actggagggg
     1861 ttggtcttcg gcatgcgggc cgcgcaatct gccgtggccc atgcgggcca tttttccgag
     1921 tcagtgcagc tgccgtcact cgaagagctc gagcaggggc gaccgactga gctcgatgat
     1981 ccggaaaagc tccgcagttc gctgcggcgg ttgatgtggg gaaaagtcgg actcgtgcgt
     2041 tcaggagatt ccttgatcag tgcctccgcc cagttggtcc gctggatgcg ccttcttggc
     2101 aaaccctttt cgtcgcgcgc tgcgctcgag gtgaagaaca tggtgcaggt ggcgcgctgc
     2161 atcacggatg ccgccttgtg gcgggaaaat agcgtcggag cccattatcg gtcggacttt
     2221 cccgaagcca agcggcaggg gtggcggaac catagccagt tactgttagg gggtggacag
     2281 gttaacggga tgccgtcgaa tgagcgagac ctcgtccttg cgaccgaggc gcctgtggga
     2341 cgtaagcccg ggaagaagac gtccgggcgt tcatgaggaa ggtgcgatag tacccgagaa
     2401 ctttgaccac atactgccga gtttcaggaa tcggtggaag cccctgatag cgttccaccg
     2461 catgttctcc cgcattgtag gccgccaggg ccaagggcag attgccgtta aatcggtcca
     2521 gcagctgccg taagtatttc gtgcccccgc cgatattctc atccggattg tatgaatccc
     2581 gcacatcgaa gcgcatagcg gtctggggca tgagctgcat caagccgacg gcgcccgcat
     2641 gtgatacggc cagtggatcg aagtctgact ctgttttgat cacggcccga atcaacgccg
     2701 gatgcaggcg atgggcgcgg gagtgccggg cgataaccgg atccaattcg cggtcggaaa
     2761 taacgctgcg cgagcgaggc agttcggaca tcacccgctt atatcgcgga tcattcggta
     2821 cattggtcag ggaaatggtg ccgtttgcgt cgatatattg gtagacctca gcctggacgg
     2881 gtgcgtgtgt cagcaagagt gttgcgtaca gcagaaggag gggcgtggtg cgtttgaatc
     2941 gggccatcgg ggttgaggcg cctgtgcctg tacggtgtgc tttgccgatg atttaaccgt
     3001 agcagtcgac ccccaacctg tcaaaaaccc ttactccttc ctacccctgg aattccctat
     3061 aaggcactga tatcacgggt aatagcggtg tgtgcgtcta aggggctggt gcaagaaacc
     3121 gggttcggct ggcagcgaaa cgcgcctgca ggcggcgagt cagggggccg ggacgaccgt
     3181 caccgatcgg ccaggcgtcg acgcgtgaaa ccggcatgac ttccatgctg gtattggtca
     3241 gaaaacattc atcagcctgg cggagatcgg ccaggtggta atggccttcc tgaactggca
     3301 ggccctcctc ttccgcgaga gtcatgacga tcgctcgcgt gataccgtcc aagataccgc
     3361 agtccaggga tggcgtatgc acggttccgt tcgagataaa aaagagatta ctgatcgtgc
     3421 attcggtcag ttcttcgcgc cagtttagaa atagactgtc aaaagtcccg gccgcgatcg
     3481 attcgcgttt cgccagaatg ttgttcagga aattcgtgga tttgatgtgc ggtgacaggg
     3541 cttcgggcag atttcgcctc gtgttggcaa cggtgagcgt gactccgtcg cgagacagtg
     3601 ccggcgggga gggatggagc accttcgtca aaatgacaac tgtcggacgg ggacagagtg
     3661 cggggtccag cccgatctct ccttcgccgc gtgaaatcgt aatgcggaga taggcatcga
     3721 cccgatcatt gcccacctca ttgcggcgca tggcctcatg gagcaggtcc ggccattgcg
     3781 cgtccggaat cggaatcgtc agcccgatgg cctctgccga gcgccgaagc cgggccaggt
     3841 gttggtcccg catgaaaatg cgagagccgt aggagcgaag cgtttcgtag acgccatcac
     3901 catagagaaa tccgtgatca aacaccgaaa cgacggcttg ttctttcggc acgaatcgat
     3961 cattcagaaa cacccacatc aacggtcagc ctccaggatg tgaagaaaag cgcccgcctt
     4021 ggcgagcgtt tcgtcatatt ctcgatcggg atccgaatcg gccacaatgc cggcccctac
     4081 ctgcaggtag ccgcggtctt tcgtgagaac caacgttcga atgagaatgt tcagatccaa
     4141 atccccgctc caactgaaat agcccagcgc tcccgtgtag aggccgcgcc gcaccggttc
     4201 cagttcgtcg atgatttcca tgcatcgtag tttgggtacg ccggtgatgg tgccgcccgg
     4261 aaacaccgct cggacgaggt cgaaaggcga ggtgcccggt cgcagggttc cgaccacctc
     4321 cgagaccaga tggctgacat gcgagtattg ctcgatggtc ataaactcgt ccacccggac
     4381 cgatccgtat tggcagactt ttcccaggtc gttccgctca aggtccacca acatgacatg
     4441 ttccgctcgt tccttggggc tggccaagag ttcggcacgc atggtcacat cgtgtgactc
     4501 gccgtttccc ctgggcctgg tcccggcgat gggtctggtg ctggcctgcg aggcggttaa
     4561 tcgcacgagt cgttcggggg aactgctgac gagagtgagc tcgggaaatc gcacgagtcc
     4621 cgcaaaggga gaggggttca cgcggcggag acggctgtag agaatgccgg cgtatcggtc
     4681 gcgatccgca gccgaccagc tagcgtccag attgagggtg aagcgatgag aaaggttggc
     4741 ctgataaatg tcgccggccg caatgtagtc ctggcagcgc cggacacgag ccgtgtaggc
     4801 gtcacgcgac tgactgggga caaagctggt ttgtatgggg ccggtcgggg cagggtgagg
     4861 ccgggatatg ctcgtcaggc gagcttccaa ctccgccaat cggtcactgc cttcccggag
     4921 cagtttttcc cgcggttcag cccgaaatcg tgacaacgga gggcagtaca ttagatacag
     4981 ctgatcggtg tggtgatcga ctgcggccac ggtatcgaag aaggccatgt gcaggtctgg
     5041 cagcccgagg tcgtcagaag ccaaggtggg cagtgattca aacgatcgga cgagatcata
     5101 actgaggtac ccgacggccc caccgtaaaa cggcggcaga ccatccggct gagggatcgt
     5161 ggaatcggcg aaggcctgcc ggaacgcatc gaacgcgtag ccatgggcgg ttgtgatgtg
     5221 cccgtcggcc ttcatggaat agtcctgcgc ccggccggtc aatgtaaggt acggatggtt
     5281 gccgaagaac gaatagcgcg cggtagcgtt ggtgccattc ccgctttcca ggagaaagga
     5341 cgggccgtcc gtaataccca ggcgctgata gagttcgaag gcgtccgcgt tcggcagcgg
     5401 tcgaaccacg accagtggtt gtggagggcc gttgagaaat gcctggtgcg atacagcagt
     5461 catgagggtg tcagtcacgg tgtcgtaccc gagaaaaaga ctataccgca tcgtgaggcc
     5521 gggtcatagc aggccaactt cttgacaagt ccccagcgaa tttgcttgaa tggcgcgccc
     5581 gccgatgggt gcactctctc cgactgtgct gaggagtgcc gcgcgtgagc acgtgatgcc
     5641 cccctctcag gatccgttat atgcggggct cgggcaagct gtccggatcg ggacggaact
     5701 gctcgctgcg ttgattgtcg g
//
LOCUS       DAOQEW010000223         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5608_length_5644_cov_7.891036, whole genome shotgun sequence.
ACCESSION   DAOQEW010000223 DAOQEW010000000
VERSION     DAOQEW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5608_length_5644_cov_7.891036"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLS35_17080"
     CDS             complement(<1..521)
                     /locus_tag="PLS35_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20438.1"
                     /translation="MFTPPHISRWMKWVGGIVVVLVTAMLLVAVIADEPARKYVEDKA
                     NASLSVYTLRIGKLDLHPLTLSLDLENVTLVQKRHPDPPMVHIPKWHAGIQLWGLLTG
                     NIVSDHRIDAPSATVTRPQAKTELKETRPSGWQDIVRNLLPLRVSDVKVKQAQVTYYD
                     HPKAQPVKLTDLQ"
     gene            complement(1204..1434)
                     /locus_tag="PLS35_17085"
     CDS             complement(1204..1434)
                     /locus_tag="PLS35_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20439.1"
                     /translation="MMLRSIRKEVLEFAKVTERLLGAASLTHELTEEECTLIALYAKD
                     LSADQQPWSKFIPGTQHQDPAYPSKGMWESSA"
     gene            complement(1814..2179)
                     /locus_tag="PLS35_17090"
     CDS             complement(1814..2179)
                     /locus_tag="PLS35_17090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI20440.1"
                     /translation="MKKRYSSRASVQSTCVLSCNGLVGQAEMVNLSVPGCLLTTRMKL
                     KVGQYVDLRLTFHTSPAPMQIKLAAVRWVSDGQVGLEFIRMSEADQTRLRWLAGHVEQ
                     RKPQANWSEPVVCLSAAYT"
     gene            complement(2434..>5644)
                     /locus_tag="PLS35_17095"
     CDS             complement(2434..>5644)
                     /locus_tag="PLS35_17095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNI20441.1"
                     /translation="KTPEDATAFHDEMCYMLAYQMAAPNSPQWFNTGLHYAYGLSGPA
                     QGHFYVDPKLGEVVRATNAFEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGS
                     GTGTNFSRLRGDGESLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVCLDLDH
                     PDIEEFIDWKVVEEQKVAAMVTGSKICAQRLNAVLKACQSVDAQGQAHLELDMKQNQA
                     LREAVTAARRDMVPEAYIHRMFDYAKQGYTHFVFHEYDTNWDGKAYQTVSGQNSNNSV
                     RIPNGFFDALEHDGDWELRRRIDGKISKTIKARDLWDKIAWAAWICADPGTQYDTTIN
                     EWHTCPEDGRINASNPCSEYMFLDDTACNLASLNLAKFFNAEGEFDLDSFRHAVRLWT
                     VALEISVLMASFPSRAIAQKSYDYRTLGLGYANLGTILMKQSIPYDSQKATAICGAIT
                     AIMTGESYATSAEMAAEIGPFPGYNRNRESMLRVIRNHRRAAYSAPHEEYEGLTILPA
                     SIQPEHCPPAMLLAARRAWDRALELGTAYGFRNAQVTVIAPTGTIGLVMDCDTTGIEP
                     DFALVKFKKLAGGGYFKIINQSLPPALQALGYTEAQIRDIVAYCAGHQTLKGAPFINH
                     EVLRQKGFDEAALERLEGSLAQAFEIQFVFNKYTLGEEFCRQKLGISEAQLADPAFNM
                     LKTLGFTQEDVAAANDYCCGTMTVEGAPHLKLEHLPIFDCANRCGRIGQRYIAVDAHI
                     RMMAAAQPFISGAISKTINMPADATLEDVKAAYLFAWKSMVKAVALYRDGSKLSQPLN
                     ASSDSGKSAEAAPSVATVAEKVAERVLVRYLHKRRSLPARRGGYTQKAIIGGHKLYLR
                     TGEYEDGTLGEIFLDMHKEGAAFRSLMNNFAIAISLGLQHGVPLEEFVEAFVFTRFEP
                     NGPVKLNDRIKMATSIIDYIFRELAITYLERKDLSQVQEDDLRMDSMKKDEQDPECVE
                     EEVDMTALKKASILTEHLPVRRNGMNGGNGHTQRAGNGSVAHKLELKRETLTVTSVRQ
                     EAKEKGYEGDPCQNCKQFTLVRNGTCLKCMSCGETSGCS"
BASE COUNT         1138 a         1654 c         1628 g         1224 t
ORIGIN      
        1 aactgcaggt cggtgagttt caccggctga gctttgggat gatcgtagta tgtgacctgg
       61 gcctgcttga ccttcacatc actcactcga aggggaagaa ggttgcggac gatgtcctgc
      121 cagccgctcg gacgggtttc cttgagttct gtcttggctt gggggcgcgt caccgtggcc
      181 gacggtgcgt cgatgcgatg gtcgctgaca atattcccgg tcaacaatcc ccacaactga
      241 atgccggcat gccatttcgg aatgtgcacc atcggaggat cgggatggcg tttctggacg
      301 agggtcacgt tttcaagatc aagcgagagg gtcagcggat gcaggtcgag cttgccgatg
      361 cgaagcgtat agacgctcag cgaggcattg gctttgtctt cgacatactt ccgcgccggc
      421 tcatccgcga taactgcgac gaggagcatc gccgtcacca ggacaacaac aataccgccg
      481 acccacttca tccagcggga gatgtgaggg ggagtaaaca caaccttacc tctctcgtgt
      541 ttaacgtgcg gaccacacac acggtagcag accggaacaa tggtccaact cggatgaacc
      601 ctagtactat gcagtcggaa gctagtgtac ccaacggtac agggtgacgc gatcgacagc
      661 gaatcttcgc ctgaacagcc gggtacggtg gtgtgtactc gcgcctcaca tgacctacct
      721 ggagttggcg cgacctgaga agatgatttg gggaaagatt agagtgaagt tcccgtcatc
      781 gccgcaatga aacggtggga tctttttgat cataggccca ggcaacgaga aagcggttgc
      841 ccctcaccgt agagtgagtc ggcaaccgca attcagtcac tgggcgtttg gaccgactcg
      901 cgtcggatca ggcaagacag atctcgcggt tgtggaactg tccaagatcg tcgccagcat
      961 cgccagcggg cgaatcttca tcatcctaca cttgggacaa atccattccg cacattgctc
     1021 gcttggcgtg tagccgagaa acagcattgc ggttgcacag taggagcatt ggagatgagc
     1081 gggagaggga ggaggcgtta tgggtgatcc gacgggaggc tgtggcggta ggggttgcat
     1141 acgctctcct ttctgcagaa tgaattctcc ggacatggat ctcactcaca ttgccattga
     1201 ccgctatgcg ctgctctccc acataccctt ggatggatag gctggatctt gatgctgagt
     1261 gccaggaatg aatttgctcc agggttgctg atctgcactc aagtctttgg cgtaaagcgc
     1321 gatcagtgtg cattcctcct cggttaattc atgagtgagg gatgcggcgc ccaggagacg
     1381 ttcagtcact ttcgcgaatt cgaggacttc tttgcggata gaacgaagca tcatcgcgtc
     1441 ccccggttga agccttttgt ccaacgacgt aggagccacg atacggcaag cgccccctgt
     1501 tgacaattgc acaaacctat tacgtagaag accgcttacc gcgaccaaga ccgcgcattc
     1561 aatttcgact agggcgagtg aaaaacgagc atcccatcat cgaagagcca tatggaaaca
     1621 acgatggaat tggtttaagc acgcgtgacg cagatactta tgcaggttca tttcgcgatg
     1681 aatgatgtgc cggaagacat gcgcctcgtg cggtgattcg cacaacttgg acctcatcga
     1741 cggaatgagg aggtgaacgg gcaacaatgt tctgctgccc gttcgtgcgt cgctcccgtc
     1801 ccttcgatga ggtttaggta taggcggcac tcagacagac cacaggctca ctccagttgg
     1861 cttggggttt tcgctgctcg acatggcctg ccagccagcg caatcgcgtc tgatccgcct
     1921 ccgacatccg gatgaactcc agccccacct gcccgtcact gacccaacgc acagcagcca
     1981 acttgatctg cataggtgcg gggctggtat ggaacgtgag ccgcaggtcc acgtactgac
     2041 cgaccttgag tttcatacgc gtggtgagca ggcagcccgg cacggagagg ttgaccattt
     2101 ccgcctggcc gactaagccg ttacaggaca acacgcaggt gctctgcact gacgctcgtg
     2161 aactgtatcg ctttttcatc cgtcctcctc tgggtgtggg ccgccgacgt cgttgatgac
     2221 ttaaagttag ccgaggaaag tcccgggcac attacctggt gttggtaact cgaaaggggg
     2281 ggaatgccag gtactgaatg ctgtttgact agctccggca gcgagtcggc ggaaaggaca
     2341 aaaatgaacc ggctgcaaga aaaggccgcg actacctcgt ttttcaacgg taatcgcggc
     2401 cttgctgttc tgctaacccg tggtcgatct acgctatgag cacccgctcg tttcgccgca
     2461 gctcatgcac ttgaggcagg tgccgttgcg caccagcgtg aactgtttgc aattctggca
     2521 ggggtcgcct tcgtatccct tttctttggc ttcctgccgc accgaggtca cggtcagggt
     2581 ttcacgcttt aattccaatt tgtgggcaac gctgccattc cccgctctct gggtatgacc
     2641 attgccgccg ttcatgccgt tccgtcgcac gggcagatgc tcagtcagga tcgacgcctt
     2701 cttgagcgcc gtcatgtcga cctcttcttc cacgcattcg ggatcctgct catccttctt
     2761 catggagtcc atgcgaagat catcttcctg cacctgggag agatctttcc gctcgagata
     2821 ggtgatggcc agttcccgga aaatgtaatc gatgatcgac gtggccatct tgatgcgatc
     2881 gttcaatttc accggcccgt tcggctcgaa ccgggtgaag acgaaggctt cgacgaactc
     2941 ctccagcggc acgccgtgct gcagaccgag ggaaatcgcg atggcgaagt tattcatcaa
     3001 gctgcggaaa gccgcgcctt ccttgtgcat gtcgaggaag atttcgccca gcgtgccgtc
     3061 ttcatactca ccggtacgca gatacaactt gtggcctccg atgatggcct tctgcgtata
     3121 cccgccgcgt cgggccggca gcgaacggcg tttgtggaga taccgcacca agacccgttc
     3181 ggcgaccttt tccgcgacgg tagccacgct gggggccgcc tccgcgctct tcccgctgtc
     3241 ggaagaggca ttcaacggct gactcaactt cgacccgtca cgatacagcg ctacggcctt
     3301 gaccatgctc ttccaggcga agaggtaggc tgccttcacg tcttccaagg tggcgtccgc
     3361 cggcatattg atggtcttgc tgatcgcgcc gctgatgaaa ggctgggctg ccgccatcat
     3421 gcggatatgc gcgtcgacgg cgatgtagcg ctgaccgatc cggccgcaac ggttggcgca
     3481 atcgaagatg ggtaaatgtt ccagcttgag atgcggcgcg ccttccacgg tcatcgtgcc
     3541 gcagcaatag tcgttcgccg ccgccacatc ttcctgggtg aaacccaggg tcttgagcat
     3601 gttgaatgcc ggatctgcca actgcgcttc gctgatgccc agtttctgcc ggcagaactc
     3661 ttcgcctaac gtgtatttgt tgaagacgaa ctggatctca aatgcctgcg ccagcgatcc
     3721 ttccagccgt tccagcgccg cctcgtcgaa acctttttga cgcagcacct catggttgat
     3781 gaacggcgcg cccttcaagg tttgatggcc cgcgcaatag gccacgatgt cccggatctg
     3841 ggcctccgta tacccgagcg cctgcagcgc cggcggcaga ctctggttga taatcttgaa
     3901 gtaaccgccg ccggccagtt tcttgaactt gaccagggcg aagtccggct cgatgccggt
     3961 cgtgtcgcaa tccatgacca gcccgatcgt gccggtgggg gcaatgaccg tgacctgcgc
     4021 gttgcggaag ccgtaggccg tgcccaactc caacgcccga tcccaggcgc gccgcgccgc
     4081 caggagcatg gccggcggac aatgttccgg ttgaatcgag gccggcagaa tcgtcaggcc
     4141 ctcatactcc tcatgcggcg cactgtaggc cgcccgccga tgattgcgga tgacgcgcag
     4201 catcgactcc cgattccggt tgtagccggg aaacgggccg atctccgcag ccatttcggc
     4261 cgacgtcgca taggattcgc cggtcatgat cgccgtgatc gcgccgcaaa ttgccgtcgc
     4321 cttctgggag tcatacggaa tgctttgttt catgagaatg gtgccgaggt tggcataccc
     4381 caatcccaat gtccggtagt cgtaactctt ctgcgcgatc gcgcggctcg ggaaagaagc
     4441 catcagcacg ctgatctcca acgccacggt ccacaaccgg acggcatggc ggaaagaatc
     4501 caggtcgaac tccccttccg cgttaaaaaa cttggcgagg ttcaacgagg ccaggttgca
     4561 ggccgtgtcg tcgagaaaca tgtattcgga acagggattc gacgcgttga tccgcccgtc
     4621 ttccggacag gtgtgccact cgttgatcgt cgtatcgtac tgcgtaccgg gatcggcgca
     4681 aatccaggcc gcccaggcga tcttatccca gaggtcccgg gccttgatcg tcttgctgat
     4741 cttgccgtca atccggcggc gcaattccca atcgccgtcg tgctccaacg catcgaagaa
     4801 gccgttcggg atacgcacgc tgttgttgga gttctgccca gacacggtct ggtaggcctt
     4861 gccgtcccag ttggtgtcat attcgtgaaa cacgaagtgg gtatagccct gcttcgcata
     4921 gtcgaacatg cgatggatat acgcctccgg caccatatcg cggcgagctg ccgtcacggc
     4981 ctcgcgcagc gcctgattct gcttcatatc caattcgaga tgagcctgtc cctgcgcatc
     5041 gaccgattga caggccttga gcacggcatt gagccgctgc gcgcaaatct ttgaaccggt
     5101 gaccatcgcg gccaccttct gctcttcgac cactttccag tcgatgaatt cttcgatatc
     5161 ggggtgatcg agatccaggc agaccatctt ggccgcgcgc ctcgtggtgc cgcccgactt
     5221 gatcgctccc gcagcgcggt cgccgatttt caggaacgac atcagaccgg aggaacgtcc
     5281 gccgcccgac agggattcac catcgccacg caaccgcgaa aagttcgtgc cggtgcccga
     5341 cccgtacttg aagagccgtg cttcacgaac ccacaggtcc atgatgccgt tctcgttgac
     5401 caggtcgtct tcaatcgact gaatgaagca ggcatgcggc tgcggatgct cgaaggcatt
     5461 ggtcgcccgc acgacctcgc cgagcttggg atcgacgtag aagtgtcctt gcgccggccc
     5521 tgaaagcccg taggcatagt gcaaccccgt gttgaaccac tgcgggctgt tgggggcagc
     5581 catctgatag gccagcatgt agcacatttc atcgtggaag gcggtcgcat cttccggcgt
     5641 tttg
//
LOCUS       DAOQEW010000224         5636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5620_length_5636_cov_7.306934, whole genome shotgun sequence.
ACCESSION   DAOQEW010000224 DAOQEW010000000
VERSION     DAOQEW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_5636_cov_7.306934"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1024)
                     /locus_tag="PLS35_17100"
     CDS             complement(2..1024)
                     /locus_tag="PLS35_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HNI20442.1"
                     /translation="MGSVKGLVLVTGAAGFIGSHVAKRLLDRGDSVLGLDNLNDYYDV
                     RLKEARLARLQTHPQFQFVKLDVSDRPGMAALFQQHSIRRVVHLAAQAGVRYSLVNPH
                     AYTAANIEGFLNILEGCRHHKVEHLVYASTSSVYGGHTKMPFSVHDNVDHPVSLYAAT
                     KKANELMAHCYAHLYRFPITGLRFFTVYGPWGRPDMALFLFTKAILEGKPIDVFNYGK
                     MQRDFTYVDDIAEGVLRTLDRPAQVDPQWSSDQPDPGSSSAPYRLYNIGNNQPVDLLH
                     FIEVLEQTLGKKAEKNLLPLQAGDVPATYADVADLMRDTGFKPATSIETGIARFVEWY
                     REYYKV"
     gene            complement(1048..2667)
                     /locus_tag="PLS35_17105"
     CDS             complement(1048..2667)
                     /locus_tag="PLS35_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI20443.1"
                     /translation="MGIWDTLPRDQYVGLAPWVWVQLESNQPPGPFPFIGGVAPEVVA
                     SLHEAHSLFLACVETAISDVFSRRATLGDPQTRVRLEDAYAELVNSRQQLSQHITARR
                     QADGQFHWSHPFDATKSATVVNTGLRIFNAVKRQAIPVPFERPMGPIVGRLLGMLDGT
                     QQAGAIQTIVATAGREGERLLTKVMELLVQYECLTPAKNTSIRSQWLSQTKDRDTIHL
                     GHAALMYRRRDQFLLFDPWLLPWFAESNVPSLWVSLLPKPAAIFLTHDHDDHVDPRTL
                     LHVPKDVPIVIPSRRNRKKYFYDYLPLLRELGFSHIIELAHGETWNFEGGAVVSVPFY
                     GEDPCDLEMPRNCYLVTDRGQNVLVHADSGPTNSGRSAIKEGVIQQLVQKYGPISLVL
                     ASQQQLQEIRSYAAHAPLSHPGLWLEVGENGYLTNSYLNELCATAQARLFVSYATGGA
                     DWYPDHLSFMFSERNPARTALLTANWEQPEKLKDLLSSHDCRYHRARALDLFRTGDDG
                     MVQVSSGVEALSPMPLYRLDHGEPAFMRRGR"
     gene            complement(2673..3104)
                     /locus_tag="PLS35_17110"
     CDS             complement(2673..3104)
                     /locus_tag="PLS35_17110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNI20444.1"
                     /translation="MPTHRGLRHLALRVTDLARSRAFYERFLAMKVVWEPDPDNVYFS
                     SGSDNLALHQIAPAELAQYQPLRGQLLDHVGVIMENPAQVDDLFRDVQQDGARYGATI
                     AKPPKQHRDGSYSFYFADPDGNVIQALYEPTISRMEFRPPA"
     gene            3276..4115
                     /locus_tag="PLS35_17115"
     CDS             3276..4115
                     /locus_tag="PLS35_17115"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI20445.1"
                     /translation="MLARSVLALVVALIGLNGTGANAFAAEHTSPPKEITGKDGAPMV
                     LIPAGSYPMGVPTGDRDGGRDEYPRHVIEIDPFYIDKYEVTNARYLEFVKATNHRVPQ
                     NPKNPTRNLWEGIGIPASLADRPVINVDWSDADAYCTWAGRRLPREAEWEKAAKGNND
                     WRFPWGNVEPTDGHLNFNQKWVGEKTLMPVGSYEKGKSPYGVYDMAGNVWEWVNDWYD
                     PKYYEKSPGKNPPGPESGTKKVIRGAGWQNETPTVRIFTRVDSDPTIRNESTGFRCAA
                     DAK"
     gene            4145..4987
                     /locus_tag="PLS35_17120"
     CDS             4145..4987
                     /locus_tag="PLS35_17120"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNI20446.1"
                     /translation="MATAGFNGLKVVSFESRMATEMTRLIERHGGKPLVAPALREIPL
                     ADNSAALQFGEKLLREGLDVLVLMTGAGTTALFEVLETRHTKSELAAALKHTVLIARG
                     PKPVAALKSLGFQPTLTVPEPNTWMDVVSTLDAHRPVNGLRVAVQEYGVSNRDLLEAL
                     KQRGAQITPVPIYRWALPEDTAPMKEALRQILAGQVQVVLITNAAQFDHLIQLAEQEG
                     ETAMFKQACGRLVLASIGPTASERIRSHGLTVDFEPSHGKMGILVKETSEQALRLLAT
                     KRAS"
     gene            complement(5046..>5636)
                     /locus_tag="PLS35_17125"
     CDS             complement(5046..>5636)
                     /locus_tag="PLS35_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI20447.1"
                     /translation="LIDWQYDGGNFQDTMVSHPVMHVSWFDAEAYCKWVGKRLPTEAE
                     WEKAARGEDGRIYPWGNQMAGLSRANFGRTGLSGPVRDRPERLLLYPPIISVDKYDNA
                     ISPYGVFQMAGNVAEWVNDWYDPKYYASAPDKNPKGPETGSQRSFRGGGWIDSTPSVR
                     VAQRNGTDPNTKMNWMGFRCARDAKEGVAGSTTEAK"
BASE COUNT         1243 a         1648 c         1631 g         1114 t
ORIGIN      
        1 ctcagacctt gtagtactcg cggtaccact ccacgaatcg agcgatgccg gtttcaatcg
       61 aggtggccgg cttgaagcct gtgtcccgca tgagatctgc cacatcggca taggtcgccg
      121 gcacatcgcc ggcctggagc ggcaggagat ttttttcagc cttcttgccg agcgtctgct
      181 cgagcacctc gataaaatgc agaaggtcga ccggctgatt attgccgata ttgtagagtc
      241 gatagggtgc agaactgcta ccgggatcag gctgatcgct cgaccactgt ggatcaacct
      301 gcgcgggccg gtcgagcgtg cgcaacacgc cttcggcgat atcgtcgaca taggtgaaat
      361 cgcgctgcat ctttccgtaa ttgaagacgt cgatcggttt gccttcgaga atcgccttgg
      421 tgaacaaaaa cagcgccata tcgggacggc cccaaggccc gtagactgtg aagaaccgca
      481 atccggtgat gggaaatcga tacagatgcg cataacaatg cgccatcaac tcgttggctt
      541 ttttggtggc ggcatacagc gacacaggat gatcgacatt gtcatgcacc gaaaacggca
      601 tcttggtatg gccaccgtag actgaactcg tggaggcata gaccagatgc tcgaccttgt
      661 gatgccggca gccttccaga atgttcagaa atccctcgat attcgcggcc gtataggcat
      721 ggggattcac gagcgagtag cggacgccgg cctgcgccgc cagatgcacc acgcgacgaa
      781 tcgagtgctg ttggaagagg gccgccatgc cgggacggtc tgacacatcc agctttacga
      841 attgaaactg aggatgcgtc tgcagccgag ccaggcgggc ttctttcaaa cgaacgtcgt
      901 aataatcgtt caggttgtcc aaccccagaa cggaatcccc gcgatccagc aatcgcttgg
      961 ccacatgcga cccgatgaat cccgcagcgc cggtgacgag caccaatccc tttacagacc
     1021 ccataacgac gcgtcccttc tgatcaatta tcttccccgc ctcatgaatg ccggttcgcc
     1081 atgatcgagg cgatagagcg gcatcggcga caaggcctcc actccggacg agacctggac
     1141 catgccatca tcacccgtgc ggaacagatc caacgcccga gcgcgatgat accggcaatc
     1201 atgtgacgac agaagatctt ttagtttttc cggctgctcc caattggccg tgagcaatgc
     1261 ggtgcgcgcg ggattgcgct cactgaacat gaaggacaga tggtccggat accaatcggc
     1321 cccgccggtg gcataggaca caaacagccg tgcctgcgca gtggcgcata actcattcag
     1381 gtaactattt gtaagatagc cgttctcacc cacctcgagc cagagtccgg gatgagacaa
     1441 tggcgcatgg gcggcataac tgcggatctc ctgcaattgt tgttgggaag ccagcacaag
     1501 ggagatcgga ccatatttct gaacgagctg ctgaatcacg ccttctttaa tggccgaccg
     1561 cccgctgttt gtcggaccgc tgtcggcatg caccagcacg ttctgcccgc gatcggtcac
     1621 gagataacag tttcgaggca tctccaaatc gcacggatct tcgccgtaaa agggaaccga
     1681 gacgacggca ccgccttcaa agttccaggt ctcgccgtga gccagttcga tgatatggct
     1741 gaaccccagt tcccgaagca atggcaggta gtcgtaaaaa tacttcttgc gattccgccg
     1801 gctgggaatg acgatcggga catccttcgg cacatgcagc agcgtcctgg gatcgacgtg
     1861 gtcatcgtgg tcatgtgtca aaaaaattgc cgccggcttc gggagcaaag atacccacaa
     1921 actcggcaca ttggattccg caaaccaggg gagcagccag ggatcgaaca gcagaaactg
     1981 gtcgcgccgc cgatacatga gcgcggcatg cccgagatga attgtgtccc ggtccttggt
     2041 ttgggagagc cactgggatc ggattgaggt gttcttggca ggcgtgaggc attcgtattg
     2101 gacgagcaac tccatcacct tggtcaacag ccgctcgccc tcccgtcccg ccgtcgcgac
     2161 gatcgtctga atggcgccgg cctgctgtgt gccatccaac attcccagca gtcttccgac
     2221 gatcggtccc atgggacgct cgaacggcac gggaatcgcc tggcgcttca cggcattgaa
     2281 gatacgcagg ccggtattga cgacggtggc ggatttcgtc gcatcgaacg gatgcgacca
     2341 atgaaactgc ccatcggcct ggcgacgcgc cgtgatgtgc tggctcagtt gttggcggga
     2401 attgacgagc tccgcataag cgtcttccaa ccgcactctg gtctgtggat caccgagcgt
     2461 cgcgcggcgg gagaacacat cgctgatggc ggtttccacg caggccagga agaggctgtg
     2521 ggcttcgtgc aaactcgcca cgacctccgg agcgaccccg ccgataaagg gaaacggtcc
     2581 cggcggctgg ttactctcaa gctgcaccca cacccaggga gccagcccga catactgatc
     2641 acgcggcaat gtatcccaaa tacccatact cgtcaggcgg gaggccggaa ctccatgcgg
     2701 ctgatagtcg gttcatataa ggcctggatg acattgccat ccggatcggc aaaatagaag
     2761 gagtagctgc catcgcgatg ttgcttcggc ggcttcgcga tggtggcccc atagcgtgcc
     2821 ccgtcctgtt gcacgtcccg gaacagatca tccacctgcg ccggattctc catgatcaca
     2881 ccgacatggt cgagcagttg tccacgcaat ggctggtatt gcgccaactc agcaggggcg
     2941 atttgatgca gggccaggtt gtcggatccc gagctgaaat acacattgtc gggatctggt
     3001 tcccatacga ccttcatggc cagaaacctc tcatagaacg cacgggaccg agccaggtcg
     3061 gtgacacgca gggccagatg tcgtaagcct cgatgggttg gcacgccggg atctcctcgg
     3121 gcgtatagta ggtggggggc tgtcaccccg tcaatcctca ttcttttgcc acgaacgtca
     3181 ttcaccccat ctttcctcca ccagtcatgt ttgaaaaatc cggagctgtg gtacgatcgg
     3241 tcatcgattc tctcgaacca aaggagcttg cagctatgtt ggcacgatcc gtcctggcat
     3301 tagtggtagc gcttatcgga ttgaatggca cgggggctaa tgcattcgcc gcggaacaca
     3361 cctccccgcc caaagaaatc acgggaaaag acggagcgcc gatggtcttg atcccggcag
     3421 ggtcctatcc catgggggtg ccgaccggcg accgtgacgg cggacgcgat gaatatccgc
     3481 gccatgtcat cgagatcgat ccgttttaca tcgataagta tgaggtcacg aacgcgcggt
     3541 atctggaatt cgtcaaggcc acgaatcatc gcgttcccca aaaccccaag aatcccacac
     3601 ggaatctctg ggaagggatc ggcattcctg catcgctggc tgaccgtccc gtgatcaatg
     3661 ttgattggtc cgatgccgac gcctactgca catgggccgg ccgacggctg ccgcgcgaag
     3721 cggagtggga aaaagccgcc aaggggaaca acgattggcg ctttccatgg ggtaacgtgg
     3781 agccgactga cggacatttg aatttcaacc agaagtgggt cggcgaaaag accttgatgc
     3841 ccgtgggtag ttacgagaag ggcaaaagtc cttacggcgt ctatgacatg gcaggcaacg
     3901 tctgggaatg ggtcaatgac tggtacgatc cgaagtacta tgagaagagt cccggcaaga
     3961 atcctcccgg gccggaaagt ggcacgaaaa aagtcattcg cggtgccggc tggcagaacg
     4021 agacgccaac cgtccggatc ttcacccggg tggatagcga cccgacgatt cgcaacgaat
     4081 caacagggtt tcgctgcgcg gcagacgcca agtagcagag tcggcgggca aggcagggag
     4141 ggcgatggcc acggctggat ttaacgggtt aaaggtcgtc tcatttgagt cgcgtatggc
     4201 gacggaaatg acgcgcctca tcgagcgcca tggcggcaag ccgctcgtgg cgccggcgtt
     4261 gcgggaaatt ccgctggcgg acaattcggc ggcactacaa ttcggcgaga agctactgcg
     4321 tgaagggctg gacgtgctgg tgctgatgac cggcgccggc acgacggctc tctttgaggt
     4381 tctcgagact cgccacacca aaagcgaact cgccgcagca ttgaagcaca ccgtgctgat
     4441 cgcccgtggg cccaaaccgg tggccgcgct gaagtcgctg gggttccaac ccaccttaac
     4501 cgtcccggag cctaatacct ggatggatgt cgtctcgacg ctggacgcgc atcgaccggt
     4561 gaacggattg cgcgtggcgg tacaggaata tggcgtatcg aaccgtgact tattagaggc
     4621 cctgaagcaa cggggagcac aaatcactcc ggtgcccatc tatcggtggg cactgccgga
     4681 agacaccgcg ccgatgaagg aagccttacg tcagattctg gcgggacaag tccaggtggt
     4741 gttgatcacc aatgccgcgc agtttgatca cctgatacag ctggcagaac aggagggtga
     4801 aaccgcaatg ttcaagcaag cctgcggaag attggtgctc gcctccatcg gccctactgc
     4861 cagcgagcgc atccgcagcc atgggctcac agtcgatttc gaaccctcgc acgggaaaat
     4921 gggtattctc gtgaaagaaa ccagcgagca ggctctgcgt cttttggcta cgaagcgagc
     4981 ctcctgaaca cacccgcctg ccaatcaggc atcattctga cacctgcacg acatcccatc
     5041 ggcaattatt tggcctcggt cgtcgatcct gctacccctt ccttggcatc tcgcgcacaa
     5101 cggaatccca tccagttcat cttggtattc ggatccgtcc cattacgctg ggcgacgcgc
     5161 acgctaggtg tgctatcgat ccagcctccg ccgcgaaagc ttcgctggct gccggtttca
     5221 gggcccttgg gatttttgtc cggggcactg gcatagtatt tcgggtcgta ccaatcattg
     5281 acccattccg ccacgttacc cgccatctga aacacgccat aggggctgat cgcattgtca
     5341 tacttatcaa ccgagatgat cggtggatag agcaacagcc gttccggacg atcccgcacc
     5401 ggcccggaca acccggtccg gccgaagttc gcccgcgaga gtcccgccat ttgattgccc
     5461 cagggataaa tgcgcccgtc ctcaccgcgg gcggcctttt cccactcggc ttcggtcggc
     5521 aatcgtttgc ctacccattt gcaataggcc tcggcatcga accaggagac atgcatcacc
     5581 ggatggctga ccatggtgtc ttgaaaattc ccgccgtcgt actgccaatc gatcag
//
LOCUS       DAOQEW010000225         5591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5686_length_5591_cov_9.341040, whole genome shotgun sequence.
ACCESSION   DAOQEW010000225 DAOQEW010000000
VERSION     DAOQEW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5686_length_5591_cov_9.341040"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PLS35_17130"
     CDS             <1..464
                     /locus_tag="PLS35_17130"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNI20448.1"
                     /translation="SFTKEGSMKMQQTVRVAIGVLVLAGALVGAGCHRHHTPAERADW
                     MTNKIAKHLDLDDQQKAKLMAVRDEMVAARAESQAEHKAIMEAAIAQVQSDRLDQAQL
                     IQLMDRHQAEQKRVMQRVLPKLTEWHATLRPEQKAEAVEHIRKWMDRYGDH"
     gene            606..1709
                     /locus_tag="PLS35_17135"
     CDS             606..1709
                     /locus_tag="PLS35_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20449.1"
                     /translation="MTIRTSMTAAAVLAVVVIAGYVLLDRLVWHNRLPDGLIQANGRI
                     EGDHVTVASKFPGRVVELAAREGAEVLKGSILIRLDDAQTKAKVDQAARLVDSLMAQV
                     EAAHTALAVLNLEVPLSIEAAAAKVESARAVIRKARAVEYEARRDAERIRALLPQQAV
                     SEQQVDQADARWKIAVTEIAVAQAAHDQAGKELAQAELGRERIRSKEAEVAALERQRD
                     QADATLAEARSVLDDLTIVAPTSGTVTTRMVDVGEVVATGAPLLELVDLDRLYLQVYV
                     PELQIGKVRLDLPARIHTDAFPDQPFDATVRYIASKAEFTPKEVQTPDERVKLIYAVR
                     LYLTTNPDHRLTPGLPADAVIRWKDDVAWSKPR"
     gene            1706..3685
                     /locus_tag="PLS35_17140"
     CDS             1706..3685
                     /locus_tag="PLS35_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI20450.1"
                     /translation="MMGPPASSADEVVRVSGFVKRYKQHRAVNGLDFTVHRGEIYGLI
                     GPDGAGKSSVMKAIAGVLGYEEGSVDVFGTLLDSERAAEQVKRRIGFLPQGLGLNLYP
                     ELSVEENIDFFAKLRLVPETDLQARKARLLSITRLDRFRSRLMKQLSGGMKQKLGLIC
                     TLIHEPELAILDEPTTGVDPVSRRDFWAILAEWQAVKGMTALVSTAYMDEAVRFHRLS
                     FLSHGRVLASGTPADVQSLVPGVVVTFESTPQLEAVERLKHRYAQVESLGPSLHVFSP
                     EQDAEAAVAGIRQVLDPLPVAHLRTDEPELEDVVVALLLKEQDGQTRASERSSQSAPP
                     ANRPQFDGPAVEAKELIRDFGSFRAVDRVSFDMQQGEIFGLLGANGAGKTTVIKMLTG
                     IVPPSGGEGRVAGADMRTASGAIKERIGYMSQAFSLYLDLTVIENIRLFADIYGLARR
                     QAQQRMAWIVEMAGLSGYEHDRTGRLPMGVRQRLALGCALVHSPRVLFLDEPTSGVDP
                     IGRRRFWDLLTRLAREEGVAILITTHYLSEAEHCDRLALMYAGHIVAEGTPADLKTQV
                     EHEVGQLLEIAVDKPGAALAQVTAAGFTGAALFGTKIHVLSRDPSRDEARLREVLGRA
                     DTTVAAVRPRMLSLEDVFVSRVMALEQAAQKKGAS"
     gene            3706..4836
                     /locus_tag="PLS35_17145"
     CDS             3706..4836
                     /locus_tag="PLS35_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20451.1"
                     /translation="MALKEWKETTRDRLFLLLAFLLPALWLVVFGYGLNLDVEDIPFA
                     VLDRDQSEFSREYLQRFIQSRYFSFRGYAHDEHSLDRLLTETKIRAAIIVPERFQEQL
                     VAGEAVAVQTLLDGTFPLHTDIAKGYVIAINQAFTEDRLIDFLRRSRGLTREQAGVLV
                     RPLSVEVRYLYNEEVRSTWSMVPALVMFTLMLASPLLTALGVVREKETGSIYNIYSST
                     VSRAEFLTGKLLPYILISLVNVCVLWLMAVGLFQVPFKGHFLLFFFASVLFVCCTTGI
                     GLLISLLVQTQMAALIITMVVAMIPTILFSGLLVPVASLTRGAKVQAHLFPAMYFTNI
                     VRGSFLKGVGVEVLWQDLLALALFAGGLGGLTYHLFTKRPKA"
     gene            4847..>5591
                     /locus_tag="PLS35_17150"
     CDS             4847..>5591
                     /locus_tag="PLS35_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20452.1"
                     /translation="MTAGQRAARWTQRLAALTWKELLQLSRDVPLLLFLIYSFSLSVL
                     VSGVGITMQLTNAELLVHDEDQSPSSRELIHRFQPPYFAVVGTINNPRQGLQQLDQGQ
                     AMVVLDVPPRFHEALVSGERTAVQLLVDTTNAPQGLSAAAYTVRIAVPFGTETALASA
                     GLSGTKMSMPLVSSAHRVWFNPTQDERWFQSISHLLRMITLFAVLLPAAALVREKERG
                     TVEQLLVSPLSPFQIMFSKVLAMAGVILLA"
BASE COUNT         1054 a         1604 c         1807 g         1126 t
ORIGIN      
        1 ggtcattcac gaaggagggc agcatgaaga tgcagcagac ggttcgggtt gcgatcggag
       61 tactggttct ggccggggcg ctggtaggcg ccggttgtca ccgtcaccac acgccggcgg
      121 agcgggcgga ttggatgaca aacaagatcg ccaaacactt agacctcgac gatcagcaga
      181 aggcgaagtt gatggccgtc agagacgaaa tggtggccgc tcgtgccgag tcgcaggcag
      241 agcacaaggc gatcatggag gcggcgattg cccaggtgca gagcgaccgg ctggatcagg
      301 cccaactgat ccaattgatg gatcggcacc aagccgaaca gaagcgcgtg atgcagcggg
      361 tgttgccgaa gctaacggag tggcatgcga cgcttcgccc ggaacagaaa gcagaggccg
      421 ttgaacatat ccgcaagtgg atggaccggt acggagacca ctgagccagc aggtcgatgg
      481 tgggccgagc tgccggaggc gcccgtctct ggcaaaaagg tcccccatcg actacagtgt
      541 cgacgcgagg gcaatggggc cttgcaggag tgattgcctc cacgcctcga ggacagactg
      601 attgcatgac tatccgcacc tccatgacgg ccgccgccgt cctcgccgtc gtcgtgatag
      661 ccggctatgt actcctcgat cgactggtat ggcacaaccg gttgccggat ggactgattc
      721 aggccaatgg ccgcattgaa ggcgaccatg tcactgtggc cagcaagttc ccgggacgtg
      781 tggtcgagct ggcggcacgt gagggcgcgg aggtgctcaa aggcagcatc ctgattcggc
      841 tcgatgatgc gcagaccaaa gcgaaggtcg atcaagccgc gcggctggtc gatagcctga
      901 tggcacaggt ggaggcggct cacacggccc tcgcggtcct gaacctggaa gtccctctga
      961 gtatcgaggc ggccgcggcg aaagttgaga gtgcgcgcgc cgtcatccgg aaggcccgcg
     1021 ccgtcgaata tgaagcccgc cgcgacgccg agcgcatccg tgccctgctt ccccagcagg
     1081 ccgtgtcgga gcagcaagtg gatcaggctg atgcgcggtg gaaaattgcc gtgaccgaga
     1141 ttgctgtcgc gcaggccgct cacgatcaag ccggcaagga actggcccag gcggaattgg
     1201 ggcgagagcg aatccggtcg aaagaagcgg aagtcgccgc gctcgaacgg cagcgcgacc
     1261 aggcggacgc cacgctggcg gaggcgcgca gcgtgctcga tgatctgacg attgtggcgc
     1321 cgacgagcgg gacggtgacc acgcgcatgg tcgacgtggg ggaagtcgtg gctaccgggg
     1381 caccactcct cgaattagtc gacctcgacc gcctgtacct gcaggtctat gtcccggaac
     1441 tccagatcgg caaggtccgt ctcgatttgc cggcccgcat ccacacggac gcgttccccg
     1501 accaaccctt cgacgccacg gtccgataca tcgcctccaa ggcggaattc acgcccaaag
     1561 aagtccaaac tccggacgaa cgggtcaagt tgatctatgc cgtccggctc tacctcacga
     1621 cgaatccaga tcatcggctg acgccgggtc tgccggcgga cgcggtgatc cgctggaaag
     1681 acgacgtcgc ctggtcgaag ccccgatgat gggcccgcct gcgtcctcag cggatgaggt
     1741 cgtccgggtg tccggcttcg tcaagcgata caagcagcat cgtgcggtca atggcttgga
     1801 cttcacggtg catcgtggcg agatctatgg cctcatcggt cccgatggtg cgggcaagag
     1861 cagcgtgatg aaggccatcg cgggggtgtt ggggtacgag gagggctccg tcgacgtgtt
     1921 tgggaccctc ttggattccg aacgggcagc ggaacaggtg aagcggcgga tcggatttct
     1981 tccccaggga ttgggactca atctctaccc cgaactttct gtcgaggaga acatcgattt
     2041 ctttgccaag ttgcggctgg ttcccgagac ggaccttcag gcgcgcaagg cacggttgct
     2101 gagcatcaca cgcttagatc gctttcgcag ccggttgatg aaacaacttt cgggcggcat
     2161 gaagcagaag ctcggcctga tctgcacgct gattcatgag ccggagctcg ccattctcga
     2221 cgagccgacg acgggtgtcg acccggtgtc gcgccgcgat ttctgggcca ttctcgcgga
     2281 atggcaggcc gtgaagggca tgaccgcgct cgtctcgacc gcctacatgg acgaagcggt
     2341 ccgctttcat cgactctcgt ttctctccca tggacgcgtg ctggcttccg gtactcctgc
     2401 agacgtgcaa tcgttagtgc ccggcgtggt cgttaccttc gaatcaacgc cgcaactcga
     2461 agcggttgaa cgtttgaaac accgctacgc ccaggtggag tcgttgggcc cgtcgctcca
     2521 cgtgttcagt ccggagcagg atgcggaggc cgccgttgca ggaatccggc aggtgctgga
     2581 tcctttaccg gtggcacatc tccgcacgga tgaaccggaa ctggaagatg tcgtcgtggc
     2641 gctcctgctg aaagaacagg acggtcagac gagagcatcg gagcgatcaa gtcagtcagc
     2701 ccccccggcg aacaggccgc agttcgacgg accagccgtc gaggctaaag agttgattcg
     2761 tgacttcggt tcatttcgag cggtggatcg agtcagtttt gacatgcaac aaggcgaaat
     2821 cttcggactc ctgggagcca acggcgcagg caaaaccacg gtgatcaaga tgttgacggg
     2881 gattgtgccg ccatcgggcg gagagggccg cgtggccggc gccgacatgc ggacggccag
     2941 cggggccatc aaggaacgta tcggctacat gtcccaggcc ttctccctgt atctggatct
     3001 gaccgtgatc gagaatatcc ggctgtttgc ggacatctac gggttggctc gacggcaggc
     3061 gcagcaacgg atggcgtgga tcgtggaaat ggcgggcctc agcggctatg agcatgaccg
     3121 gacaggtcgc ctgcccatgg gggtgcggca gcgtctcgcg ttggggtgcg cactcgtcca
     3181 cagcccccgg gtcttgttcc tggacgaacc gacgtccggc gtggatccca tcggccgtcg
     3241 tcgattctgg gatttgctga cgcggttggc ccgcgaggaa ggcgtcgcga tcctgatcac
     3301 cacccattat ctgagcgaag cagaacattg cgaccggctc gcgctcatgt atgccggcca
     3361 tatcgtggcc gaagggacac cggcggatct caaaacccag gtggaacacg aagtggggca
     3421 actgctggag attgccgtgg acaagccggg tgcggcactg gcgcaggtga cggccgccgg
     3481 gttcaccggt gccgcgctct tcggcaccaa gatccatgtg ctctcgcgcg atccttctcg
     3541 tgacgaggcg cgtctgcggg aggtgttggg ccgcgctgac acgacggttg cggccgtgcg
     3601 tccacggatg ctcagcttgg aggacgtgtt tgtttcacgt gtgatggcgc tcgaacaggc
     3661 ggcgcagaag aagggggcgt cctgaacctg caacgcatca gcgctgtggc gctcaaggag
     3721 tggaaggaga ccaccaggga ccggctgttc ctcctgttgg cctttctcct gccggctctc
     3781 tggctcgtgg tgttcgggta cgggctcaat ctggacgtcg aagacattcc ctttgccgtg
     3841 ctggatcggg accagagtga gttcagccgg gagtatctcc agcggttcat tcagtcgcgg
     3901 tatttttcat ttcgagggta tgcccacgac gagcacagcc tggatcggct gttgaccgaa
     3961 acgaagattc gggcagccat catcgtcccc gaacggtttc aggaacaatt ggtcgccgga
     4021 gaagcggtcg ccgtgcagac attgctggat gggacctttc cactgcacac ggatatcgcc
     4081 aagggctacg tcatcgcgat caatcaggcg tttacggagg atcgattaat cgatttcctg
     4141 cggcgctcgc gcggcctcac ccgggagcaa gccggcgtcc tcgtgcggcc gctgagtgta
     4201 gaggttcggt atctgtacaa tgaagaggtc cggagtacct ggtccatggt accggcgctg
     4261 gtcatgttca cgttgatgct cgcctcccct ctgctgacgg ccctcggggt tgtgcgggaa
     4321 aaagaaaccg gctcgatcta taacatctac agctccacgg tcagtcgagc cgaatttctc
     4381 accgggaagc tgctccccta cattctcatt tccctcgtga atgtctgcgt gttgtggctg
     4441 atggctgtgg gcctgtttca ggtgccgttt aaaggacact ttcttctctt ctttttcgcg
     4501 tcggtgctgt tcgtctgctg taccacggga atcggcctgc tgatttcgct gctggtgcag
     4561 acgcaaatgg cagccttgat catcacgatg gttgtggcca tgatcccgac cattttgttt
     4621 tccggccttc tggttcccgt tgcctcttta acacgagggg cgaaggtgca ggcccatctt
     4681 ttcccggcca tgtacttcac caacattgtg cgaggcagtt ttttgaaagg cgtcggcgta
     4741 gaggtcctgt ggcaggatct actggcgctg gccctctttg ccggcgggtt gggtgggctt
     4801 acgtatcatc tcttcaccaa gcgacccaaa gcatgagggg tatggcatga cggccgggca
     4861 gcgggcagcc aggtggacac aacgacttgc ggcgctgacg tggaaggaac tcctgcaact
     4921 gtcgcgcgat gtaccgttgt tattattcct gatctattca ttttctctgt cagtgctggt
     4981 cagcggagtc gggattacga tgcagctcac caatgcggag ctgctggtgc atgatgagga
     5041 tcaaagtccc tcttcacgag agctgattca tcggtttcag cccccctatt ttgctgtggt
     5101 gggcacgatc aacaacccgc gccaagggct tcagcaactg gatcagggcc aggcgatggt
     5161 ggtgttggat gttcctccga gattccacga agccctcgtg agcggggagc gaacggcggt
     5221 tcaactgctg gtcgatacta ccaatgcgcc acagggactt tccgccgccg cctataccgt
     5281 gcgcattgct gtcccgttcg gcaccgagac ggctctggcc tctgcaggtc tgtccggaac
     5341 gaagatgagc atgccgcttg tctccagtgc gcatcgtgtc tggttcaatc cgacccagga
     5401 cgaacgatgg ttccagtcca tttcgcatct gttgcggatg atcacgctct tcgcggtgtt
     5461 gttgccggcc gccgcgttgg tgcgtgaaaa agagcgaggc acggtcgagc agttattggt
     5521 gtccccattg tccccgtttc agatcatgtt ttcgaaagtg ctcgccatgg ccggggtgat
     5581 tctcctcgcg a
//
LOCUS       DAOQEW010000226         5533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5788_length_5533_cov_8.531763, whole genome shotgun sequence.
ACCESSION   DAOQEW010000226 DAOQEW010000000
VERSION     DAOQEW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5788_length_5533_cov_8.531763"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(910..1746)
                     /locus_tag="PLS35_17155"
     CDS             complement(910..1746)
                     /locus_tag="PLS35_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20453.1"
                     /translation="MAELTVTGLCIRWAPVNDAESFSIGGPGIYAALGARLVNAVPTL
                     GLRLGIDVSHRELVFITRLGSLSSEIERVEQANLLWQGPILLDQTRVGMAVGEPTSMS
                     PAQLPGSDGWLTVANLDPSAILSAVGLWTGPVAVDCYEPWIRFRPKSSIALASRADVL
                     FLTERELAAFPGNAQRFILEAKDRFVVLKKGSRGVSLYHNGHERQLPAPTPLVIRSDL
                     GAGDLLLGLLSGHLAGASGPNDALTIDNLEEAYFTVRPWLSELLSASHPIEFLARHEY
                     LA"
     gene            complement(1739..2641)
                     /locus_tag="PLS35_17160"
     CDS             complement(1739..2641)
                     /locus_tag="PLS35_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20454.1"
                     /translation="MLAQSLEAPLACDLKLDIEAVGIVAPGGQAEAVTKLHNAQERTR
                     LAGLLQHCAPRRIPPSSQPQIPRQLARGSGVSRASSAPRASVYACLASKSALDGIDGQ
                     DETTAVIALSTTAALDAALRYELSGLEYGWETVDPFLLPYAIPSAMATQVAMACGANE
                     GAICLFDGFYSFPHALTLSAIYLRSAVRRVVIVAAEQAVPALTQAMGCQGMDDLMEGA
                     VAFVVRQPVDHAAAVWLRVRQHHPRGLGDHGATPVPTVGTSLDDMLSVCSLIASLPEV
                     ATSIDTPWIRSRSGAVELLAAPHG"
     gene            complement(2604..3785)
                     /locus_tag="PLS35_17165"
     CDS             complement(2604..3785)
                     /locus_tag="PLS35_17165"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNI20455.1"
                     /translation="MRDEAVCILQEPIWAACNLGQTWPQIWSNLVAGTSNLEPAHNHV
                     PRVHGEIHVGATALANGSYWERGRRLARATADVVIGSIRALHGKRADIRCVILVATSH
                     GDPGPSAACAEATLGFHADWEQVCPILSDAISAGIRDAAGRFSHVYTVAAACASGVVA
                     LDLGALMLEFDQADLVYVVALDALSRVAYTGFKQAGAMTRNMARPFDIDRDGTMLGEA
                     AVVFAMSLARTARALRLEPTAVIEASAQQCDASHLVEPSATGVMKTISKALERAGRQP
                     HELAAVYWHGTGTRQNDDAEASAAQAIFGATVPPGTSTKGALGHCMGASGAINVLAAC
                     QSLKDARIPATAGLRTAEVGSLSIVHGAPAQIGPGPILVNSLGFGGINACAIIGSAAG
                     V"
     gene            complement(4273..4527)
                     /locus_tag="PLS35_17170"
     CDS             complement(4273..4527)
                     /locus_tag="PLS35_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNI20456.1"
                     /translation="MSDDLPVMKVILESALDSELPVNELTLSHHLYEDLGMDSMAAVV
                     MVVEIQKRFGIRIADDDVPRLMTVGSVLDKVEALRRVRHA"
     gene            complement(4558..5307)
                     /locus_tag="PLS35_17175"
     CDS             complement(4558..5307)
                     /locus_tag="PLS35_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI20457.1"
                     /translation="MDDTMNRPCIVVGASGDLGAAVARRLLRENRRMVLTSTQLANPK
                     VVQVLQDKASSDRVMWSQMDVRDTASVNAGIESARSFLGPSYDLVYCAGIVRDAPIAL
                     ASDESWESTFQINLRGAFFVLRAAARDLMVAGNARVVLVSSVSGQLANPGQAAYAASK
                     AGLDALARVAASEFGRFGVCVNTIAFGAMEGSLIRSANYKLPAKVAEVAPLRRLASSD
                     ECAEAVAALLGPAGSYITAQAVVVDGGLSAV"
     gene            complement(5326..>5533)
                     /locus_tag="PLS35_17180"
     CDS             complement(5326..>5533)
                     /locus_tag="PLS35_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20458.1"
                     /translation="DDFISACWDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAQ
                     FRRALHEIADGKITELGPEFSDFG"
BASE COUNT         1144 a         1784 c         1566 g         1039 t
ORIGIN      
        1 cgcctgtgcg cgattaagaa acgatcatgg caacgatgaa acggcgcgtt gacaggccgc
       61 tttcgagcca gaccgcggtc ggcggcaggc ggccacctgc tgccgttgcc cattccgtcg
      121 caccggtcat ccaggcgacc gctgcgctcc gacatctgcc agtcgcccaa cttcggcggc
      181 catctgttga tcgacgtttg ccatcggttg aatctgaaat ttgagcggct ggcttaacca
      241 gtacatgccc cacatcgacc ccaacgtcgg cagcctgccg tcattaacga catctcacga
      301 acccgttgcc aggcttacgt cacaagcccg gatcacaaca cggaagtgtc agggcaatcg
      361 cgtatcctgc ccagccacgt gcatacccag tcaattgcgc catcagttgg ccgcgtgcgt
      421 tccagatagc tgcaccgctc attccttcca cggggcgtat tgagagtacg agcattcgcg
      481 gtagcgtgcg ccatcgcagc tttcggcatc tgtgcgccag gagacttgct ggcggccgcg
      541 aaaccgcaag cacacgagca ttcatacgac gttcgactag attcgccccg cacggccaag
      601 cgaattccac ggtgactagc tggcctgctg tcggccgact ccgttgggtc gcccaacgcc
      661 gagtagaggc gctgtggtgt cctagtagag ccacgtcaaa ttcgggccag cagccaatgc
      721 agtcgacggc gccgtgttgg cctctcatgc cgagcagtag ttcgggcgcc cgaatcgtgg
      781 ccacatttaa caccctcccg cggtgaacgt tcagtgcgtg tagcggtgcc tgccagcaac
      841 cctgtcgtcg gatcgcctga cagactacgt ttccactccg atccaagagc gcatgcacgc
      901 gaccgaacgt cacgccaaat actcgtgccg agctaggaac tcgattgggt gcgaagcact
      961 gagcaactcg gagagccacg gcctcaccgt aaagtacgct tcctccaagt tatctatggt
     1021 cagcgcatcg ttggggcccg aggccccagc tagatgaccg gatagcagcc ctagcagaag
     1081 gtcaccagcg cccaggtctg aacggatgac caatggagtg ggagctggta gctgtcgctc
     1141 gtgaccgttg tggtatagcg agacacccct cgagcccttc ttgagcacca cgaatctgtc
     1201 cttggcctca agaatgaacc gctgagcatt tcccggaaaa gccgcaagct cacgctctgt
     1261 caagaacaat acgtccgcgc gactagccaa agctatgctg ctcttcggac gaaatcgaat
     1321 ccatggttca tagcagtcaa ctgcgaccgg gccagtccac aaaccgacgg ccgaaagaat
     1381 cgcgctggga tcgagattgg caacagtgag ccaaccgtca gatcctggaa gttgcgctgg
     1441 gctcatggaa gtcggctctc cgaccgccat gcccactctg gtttgatcaa ggaggatagg
     1501 gccctgccac aacaaattcg cctgttcgac tcgttcgatt tctgacgaga ggctccccaa
     1561 cctcgtgatg aataccagtt ctcgatgaga gacgtcgatg cccagccgaa gacccagggt
     1621 gggtacagca ttaaccagcc gcgcacccaa agccgcatag atccccggac cgccaatgga
     1681 gaacgactct gcgtcgttca ccggtgccca acgaatgcaa agaccagtga cggtcagctc
     1741 agccatgggg cgcagcaagg agttcgactg cacctgagcg ggatctgatc caaggtgtat
     1801 cgatgctagt tgccacttcc ggcaaagaag cgatgagact gcacacactc agcatgtcgt
     1861 ctagtgaagt gccgacggtc ggcacaggcg ttgccccgtg gtcgccaagc cctctagggt
     1921 gatgctggcg tacccgcaac cacactgccg cggcatgatc gaccggctga cggactacaa
     1981 aggcaaccgc tccctccatg aggtcatcca tcccctggca ccccatggcc tgcgtcagcg
     2041 ctgggaccgc ttgctcagca gcgacgatga cgacccgccg caccgccgac cgaaggtaaa
     2101 ttgcgctaag cgtgagcgcg tgcgggaagg aatagaatcc atcgaatagg cagatggctc
     2161 cctcgttggc gccacacgcc attgcaacct gtgtcgccat cgcgctcgga atcgcgtacg
     2221 ggagcaagaa ggggtcaacg gtctcccatc cgtattcgag cccgctgagt tcgtatctca
     2281 gtgccgcatc cagcgctgcg gtcgtactca gtgcaatgac ggccgttgtt tcatcctgac
     2341 catcgatgcc atccagcgcc gacttgctgg ccaaacaagc gtagacagag gcgcgcggcg
     2401 cggaactcgc gcgagagact cctgaccctc gggccagttg gcgagggatc tgcggctggc
     2461 ttgacggcgg gatgcggcgc ggtgcgcagt gctgcaacag ccctgcaagc cgcgtacgtt
     2521 cctgcgcgtt gtgcaacttg gtgaccgctt cggcctgccc gccgggcgca acaatgccca
     2581 cagcctcaat atcaagcttt aggtcacacg ccagcggcgc ttccaatgat tgcgcaagca
     2641 ttgatgcctc caaacccaag cgagttgacg aggatgggcc caggtccaat ctgtgccggg
     2701 gccccgtgga caatcgacag cgagccgacc tctgcagtgc gcaaacccgc tgttgccgga
     2761 atcctggcat ccttgagcga ctgacatgca gccagcacat tgattgcgcc ggacgccccc
     2821 atgcaatgtc ccagtgcgcc cttcgtcgag gttccagggg gcaccgttgc gccgaagatc
     2881 gcctgagctg ccgaagcctc agcatcgtcg ttttgacgcg ttccggttcc atgccaatac
     2941 actgcagcga gctcgtgcgg ttgccgacct gcgcgttcca gtgccttcga gatcgtcttc
     3001 attacgcccg tggcactggg ctccactagg tgactggcgt cgcattgctg agcactggct
     3061 tcaatgacgg cggttggctc aagccgcagt gctcgagccg tgcgcgcaag cgacattgcg
     3121 aagactactg ccgcctcccc caacatggtc ccatcccggt cgatatcaaa cggtctcgcc
     3181 atgttgcggg tcatcgctcc cgcctgcttg aatcccgtgt acgccaccct cgaaagcgca
     3241 tctagtgcga caacgtacac cagatcggct tggtcgaact cgagcattaa agccccgagg
     3301 tcaagggcca ccacaccgga ggcgcaggcg gctgcaaccg tatacacgtg agagaacctg
     3361 ccggcggcgt ctcgaatgcc ggctgagatg gcgtcggaca gtatgggaca aacttgctcc
     3421 cagtccgcgt gaaacccaag tgtcgcctcg gcacaagctg cagacggacc cgggtcaccg
     3481 tggctcgttg cgaccaggat tacgcatcga atgtcggcgc gcttaccatg caatgcacgt
     3541 atcgagccga tgacgacatc tgcggtggcg cgtgcgagcc tgcggccccg ctcccagtag
     3601 gaaccgttgg ccaatgcggt cgccccgaca tggatctcac catgaacccg ggggacatgg
     3661 ttatgcgcag gctccagatt cgaggtgcct gccacaaggt tcgaccagat ctgtggccaa
     3721 gtttgaccga gattgcatgc agcccagatc ggttcctgga ggatgcagac cgcttcgtcg
     3781 cgcatgacga gctcctagat ttttgcggct gcacccagca gctcgaccgt acacacagcc
     3841 ctttcgccgc acgtcacaat cccgcggaac agcgatgtag atccaacggt atcctgtctt
     3901 ccaactctga ccgttaggac atcgccgatt cgagcaggcg agtgaaaact tgccttgcat
     3961 cgggccagga gatgcgcctc cgggagcgag agggcagatc gttgtggaag ttgctcacgc
     4021 gcgaaaactg tttgtagcag tgtggcaacc tgtccgacca tctctatcag aagcaccccg
     4081 ggaaagatcc tgcccttgcc cgcaaaatgg gctcgcacga atggatctcc ctcggcgatg
     4141 gttcgagtgg cctgaattga attgggagga tcgatcgagt cgattcggtc gacgaagatc
     4201 caggggggaa gatggggcaa gtaactctct ggggcgtatg ccgagccaat ccccgaaggc
     4261 acatcagggg cgtcaggcat gtcggaccct cctcagagct tcgaccttgt ccagcactga
     4321 accgactgtc atcagacgag gaacgtcatc gtcagcaatg cgaatgccaa agcgcttctg
     4381 aatttcgacc accatgacca cggcagccat cgagtccatg cccagatctt cgtagagatg
     4441 atggcttaac gtcagttcat taaccggcaa ctcactatcc aatgccgact caaggatgac
     4501 tttcatcact ggaaggtcgt ccgacatatc atcctcactt gtctacctga agaggggcta
     4561 tacagcggaa agcccgccgt ccaccacgac ggcttgggct gtgatgtagc taccggcggg
     4621 gcctaacagc gccgcaaccg cctccgcgca ctcgtcgctg gatgccaggc gccgcagtgg
     4681 tgccacttcc gccaccttag ctggaagctt gtaatttgcg gagcgaatga gactgccctc
     4741 catcgcgccg aaagcgatgg tgttaacaca cactccgaat cggccgaact cgcttgccgc
     4801 gactcgtgcc agcgcgtcta gaccagcctt cgaagccgca tacgctgcct gtcccgggtt
     4861 tgccagttgc ccggacacag agctcacaag cacgactcga gcgttgccgg ccaccatcag
     4921 atctcgcgct gcagcccgaa gcacgaagaa cgcgcctcgc agatttatct ggaaggtgga
     4981 ttcccaagac tcgtcgctag caagcgcgat cggtgcgtct ctgacgatac ctgcgcagta
     5041 gaccaaatca taggaaggtc ctaggaatga cctcgctgac tcgataccag cgttgacgct
     5101 ggcggtgtct cttacgtcca tttgggacca catcacccta tcggagctcg ctttatcctg
     5161 caggacttgg actaccttag ggtttgccag ctgagtgctg gtcagcacca tgcggcgatt
     5221 ctcgcgcagc agtcgccgag cgactgctgc gccgaggtcg ccggaggcgc cgaccacgat
     5281 gcatggccgg ttcatcgtgt cgtccatgct gcacctctca gtcggctagc cgaaatcact
     5341 aaactccggg cccagttcgg taatcttgcc gtcagcgatt tcatgaagtg cccggcggaa
     5401 ttgcgctccg cctccgccgc ctccgcctcc gccgcctccg cctccgcctc cgccgcctcc
     5461 gcctccgcct ccgcctccgc cgcctccgcc gcctccgccg cctccgtccc aacaagcaga
     5521 aatgaagtca tcg
//
LOCUS       DAOQEW010000227         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5806_length_5517_cov_9.846393, whole genome shotgun sequence.
ACCESSION   DAOQEW010000227 DAOQEW010000000
VERSION     DAOQEW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5806_length_5517_cov_9.846393"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..749
                     /locus_tag="PLS35_17185"
     CDS             375..749
                     /locus_tag="PLS35_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HNI20459.1"
                     /translation="MTVPVPSKGEKEAAVRTAIIKFEQEFLGRGPDEVRVFIVRDMLV
                     VRLKGVLTPAERQLAKTAEGVDMVKRLRQNLIAQGRERLCEQVADLIGAKITALFTDI
                     DTVVGERIFVFTLESDLEANFR"
     gene            773..2248
                     /locus_tag="PLS35_17190"
     CDS             773..2248
                     /locus_tag="PLS35_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNI20460.1"
                     /translation="MTQHSLESLTFENSYARLPEVFYAKVNPTPFRAAPFLISANPAA
                     MELIDLDPQEALRPEFAGVFGGSLLVPGMEPLAMLYSGHQFGVYVPQLGDGRAILLGE
                     IVNGRGERWDLHLKGAGMTPFSRDGDGRSVLRSAIREYLCCEAMHGLGIPTTRALCLV
                     GSDDKVYREQVETGATIVRMAPSHVRFGTFEIFYYRKQHEHLQRLADYVIEMHFPDLA
                     QAADKYVRFFAAVVERTAKLIAQWQAVGWSHGVLNTDNMSILGLTLDYGPYGFMDDYD
                     PGFICNHSDYNGRYAFNQQPYIGLWNLSCLAQTLLPFAPKEDLKAALDGYQATVDRYY
                     RDHMRQKLGLREDRGEDEALLQELKSLMVGSRVDYTIFWRELGTFSSDPAAKNERLRE
                     HFLNPDRFDVWAAQYRERLHSEQSRDEERRAGMDRINPKYVLRNYLAQTAIEKAQQKD
                     YSEIDRLLNLLQDPYRDHPGMESYAAAPPNWGKHLSVSCSS"
     gene            2295..3008
                     /locus_tag="PLS35_17195"
     CDS             2295..3008
                     /locus_tag="PLS35_17195"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20461.1"
                     /translation="MRFEKLGGLTVRLTGGTDGKGGGNGPWVMLLHGFGALGDDLVPL
                     SEYLDAPAGTRFLFPEAPIPIPMGFGDSRAWWMIDMARIQADRAAGKIRDMSGEIPRG
                     LPEARERVKALLEDVHKKLGADPARTLLGGFSQGAMLSCDALLHSTLPYAGLIQLSST
                     LVARQEWAPLLTKRKGLPLYQSHGTQDPILPFTMAERLRDEFHLAGLVVDWQPFRGGH
                     EIPEPILRRLGSFILKTLV"
     gene            3110..3820
                     /locus_tag="PLS35_17200"
     CDS             3110..3820
                     /locus_tag="PLS35_17200"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI20462.1"
                     /translation="MMMAHDRSRTEYLSTQSAYDEWAEDYDDGDPTVLLDQPFLLSML
                     QPFAGCRILDLGCGTGRYLRLASHEAALVGLDLSRGMLERARRQTPPATGATWVQGSV
                     ERIPFTSESFDRIVSGLVLDHVHDLAEFFRGIGTMLRPGGRAIVTAVHPEMQRKTGPT
                     VRFTAAGREYRTEGTIHEKAAIITALQEAGLLIEQLQEPPVTERLIARREAWKDRLGC
                     PALLLLALTRAADSRHDH"
     gene            3810..4565
                     /locus_tag="PLS35_17205"
     CDS             3810..4565
                     /locus_tag="PLS35_17205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI20463.1"
                     /translation="MTIDATMFELQGTDHKRLRGNRILGKDRQILFITGFLSKRWGNK
                     SKALAQWCQDRGWGFCCFDFRGWGDSEGQWGDYRLLHWLEDAEAVTNLLADGPPVTIV
                     GNSLGGWLAWLVAQEQPAVEELILIAPAFNMMDLRAAQISAERREQWQSAGSMPWDDE
                     PLHREAPIPWHWVEDSQTLWRRRLTMPRRVKTTILHGLQDTVIKPEGSWAFVQHVLSQ
                     DPEFPVELLLKTGDHRLSSPEDLDTFRRLVVKE"
     gene            4568..4735
                     /locus_tag="PLS35_17210"
     CDS             4568..4735
                     /locus_tag="PLS35_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20464.1"
                     /translation="MLMLIHKECGGPALEESPVGEVCAIPVDRFPFSCFSCLEEIIDE
                     SELRLIEDLGI"
     gene            4760..>5517
                     /locus_tag="PLS35_17215"
     CDS             4760..>5517
                     /locus_tag="PLS35_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNI20465.1"
                     /translation="MSSLGAHNSGPDSEGPDVPEPSHAEKARTLVHLQQTGGLSTLSR
                     KQPGWPFGSVMPYGLDDQGQPSFLISTMAMHTQNLLGDPRASLLVTPPESQRDPLGAA
                     RVTLMGSVTKVPKDEAAPVRERYLARHANAAYWVDFDDFAFFRMALADIYFVGGFGSM
                     GWVAPAEYLAAAVDPLAETAADLIREMNTTQQETLLLLARECGNLDAQQASITTMDRL
                     GFHLRVKTPERMQGGRFAFTNPVRTAQEARAGVA"
BASE COUNT         1128 a         1550 c         1721 g         1118 t
ORIGIN      
        1 ccatgaaaag aaaaaggcca gccgacggtc cttcacacaa ggaaccaacg actggcctgc
       61 actgtccccg gcctttcccg caaaggaaac accggcaaga ccctactgcc tgtctgagaa
      121 catcaatgga tgtgggtccg ggccgcgcac gaatcacggc ctgtcgtcgg caccaccgga
      181 aaactgggtt agatgtatct gtgaaaccca cgcaattgca agagaaaagt ccgcgcaggc
      241 gaaccggccg catgggcgcg ccacacttga tcgcggctcg gattgccggg tgtcggggtg
      301 tcacatgggg gtggagtcct gcggcggccc tgactataat ccggtcgtcc atgtctgtat
      361 caccagggag caagatgacc gtgccggtgc ccagcaaggg agaaaaggaa gccgccgttc
      421 gtacggcgat catcaaattc gaacaggaat ttctggggcg cggccccgac gaggtccgtg
      481 tgttcatagt gcgggacatg ctggtcgtgc ggctgaaggg ggtgctcacg ccggctgagc
      541 gacaactggc caaaacggcc gaaggcgtcg atatggtcaa gcggctgcgt cagaacctga
      601 tcgctcaggg gcgtgaacga ctgtgtgaac aggtggcgga tctcatcggt gccaaaatca
      661 cggcgttgtt caccgacatc gatacggtgg ttggtgagcg catctttgtc ttcaccctgg
      721 aatccgatct cgaggccaat tttcggtgag gtgaggaagg gtgcaaaagc caatgacgca
      781 acacagcctg gagtccctca cgttcgagaa cagttacgca cgcctgccgg aagtgttcta
      841 cgcgaaggtg aatccgacgc cgtttcgcgc cgcgccgttt ttgatcagcg caaatcccgc
      901 ggcgatggaa ttaatcgatc tcgacccgca ggaagcgctg cgaccggaat ttgccggagt
      961 cttcggcggc agtctgctgg tgccggggat ggagccgctg gcgatgttgt attccggcca
     1021 tcagttcggg gtgtacgtgc cccagctcgg cgatggccgt gcgattcttc tgggggaaat
     1081 cgtcaacggg cgaggggagc ggtgggatct gcatttgaaa ggcgctggga tgactccctt
     1141 ctcccgcgat ggagacggac gctccgtcct tcgttcggcc attcgtgaat atctctgctg
     1201 cgaagcgatg catggactcg gcatcccgac cacacgcgcg ctctgcctgg tcggcagcga
     1261 cgacaaggtc tatcgtgaac aggtggaaac cggcgccacc atcgtccgca tggcgccttc
     1321 gcatgtgcgg ttcggcacct tcgaaatttt ctattaccgg aagcagcatg agcacctgca
     1381 gcgattggcc gattacgtaa tcgagatgca tttccctgat cttgcgcagg ctgccgacaa
     1441 atatgtccgc ttttttgccg ccgtggtcga gcgcacggcg aaacttatcg cgcagtggca
     1501 ggcggtcggc tggtcccacg gcgtgttgaa taccgacaac atgtcgatcc tcggactgac
     1561 cctggactat gggccctatg gcttcatgga cgattatgat ccgggcttca tctgcaacca
     1621 ttccgactac aatggacgct atgcgtttaa tcaacagcct tacatcgggc tgtggaacct
     1681 gagttgcctt gcacaaaccc tattgccgtt cgcgcccaag gaagacttga aagcggcgct
     1741 cgatggctac caggctaccg tcgaccggta ttatcgggat cacatgcgac agaaactcgg
     1801 cctgcgagaa gatcggggag aggatgaggc gctgctccag gaattgaagt cgcttatggt
     1861 ggggagtcgg gtggattaca cgattttctg gcgcgaactc ggcacctttt catccgatcc
     1921 tgcggcgaag aacgaacgcc tgcgggagca tttcctcaat ccggaccgct tcgatgtctg
     1981 ggccgcacag tatcgtgaga gactgcacag cgaacagagt cgcgatgaag agcgacgggc
     2041 aggaatggat cgcatcaatc ccaagtatgt cctccgtaac tatctggctc aaacggcgat
     2101 cgagaaggcg cagcagaaag actattctga gatcgaccgc ctcctgaacc ttcttcagga
     2161 tccgtataga gaccatcccg gcatggaatc ctacgcagcc gcgcccccga actggggcaa
     2221 gcatctcagt gtgagctgct cgtcctgacc gacccggttc gacccgttct ctgataggaa
     2281 gatgagggca tgtgatgcgt tttgaaaaac tcggcggcct gacggttcgt ctaacaggcg
     2341 gaacagacgg caagggcggg ggcaatggcc cctgggtgat gttgctccat ggattcggcg
     2401 cattgggcga tgacctggtt ccgctcagtg aatatctgga cgcacctgca ggcacgcgct
     2461 ttctctttcc cgaagcgccg atcccaattc cgatgggctt cggcgattcg cgcgcctggt
     2521 ggatgatcga tatggcccgc attcaggccg atcgggcggc ggggaagatc cgcgacatgt
     2581 caggcgaaat cccgcgtggc ctgcctgaag cccgtgagcg ggtgaaagcc ttgctggagg
     2641 atgtacataa gaaactcggg gctgatcctg cgcggactct gctgggagga ttttcccaag
     2701 gcgcgatgtt gtcctgtgat gcgctgctgc atagcacgct tccatacgcc gggctgatcc
     2761 aactctcaag cacgctggtg gcaaggcagg aatgggcgcc tctgctcacg aaacggaagg
     2821 ggctgccact ttatcagagt catggcacgc aggatcccat tctgccgttc accatggccg
     2881 agcggttgcg ggatgaattc catctggcgg gcctggtcgt cgattggcag ccgttccggg
     2941 gagggcatga aattccagag cccattctgc gccgtctcgg aagcttcatc ctgaaaacgc
     3001 tcgtgtaaca cattgtgcaa gctctaggga aaaccgtctc tgattgcatc ccgaacccct
     3061 cccgaatgga gccgtctgct gaaagggtgc cgctgggtcc tatatggtga tgatgatggc
     3121 acatgatcgc agcaggacag agtacctctc cacccagtcg gcctacgatg aatgggccga
     3181 ggattatgat gacggggatc ccaccgttct tctcgatcag ccatttcttc tgtccatgct
     3241 gcagcctttt gcaggatgcc gaattctgga tctggggtgc ggaaccggtc gctatcttcg
     3301 tttggcgagt cacgaagccg cgctggtcgg cctggatttg tcccgaggca tgctggagcg
     3361 cgccaggcgc cagacaccac cggccactgg agcgacctgg gtgcaagggt ctgttgagcg
     3421 catcccgttt acttcagaga gcttcgaccg tattgtctcc ggtttggtgt tggatcatgt
     3481 tcacgacctg gcggaatttt tccggggcat cgggaccatg ctccggccag gcgggcgtgc
     3541 gatcgtgacg gcggttcatc cggagatgca acggaaaacc gggccgacgg tgcggttcac
     3601 cgccgcaggg cgtgaatatc gtacggaagg aaccatccac gagaaggccg ccattatcac
     3661 agcccttcag gaggcagggc tcttgatcga gcaactgcag gaaccaccgg tgacagagcg
     3721 actcatcgcc cgccgggagg cgtggaaaga tcggctcggc tgtccggcac tgttattatt
     3781 ggctctgaca cgtgcggcgg attctcgtca tgaccattga tgccaccatg tttgaattac
     3841 agggtaccga tcacaagcgc ctgcgcggga accgcattct aggcaaagac cgccagatct
     3901 tgttcatcac ggggtttctc tcgaagcgct ggggcaacaa gagcaaggca ctcgcgcaat
     3961 ggtgccagga ccgaggatgg ggattttgtt gtttcgactt tcgcggctgg ggtgactccg
     4021 aagggcagtg gggagactat cgcctgctcc actggctcga ggatgcggaa gccgtcacga
     4081 atctgctggc cgatggtccg ccagtgacga tcgtcggcaa ttccctcgga ggttggctgg
     4141 cgtggctcgt tgcgcaagag caaccggccg tcgaggaact cattctgatc gccccggcct
     4201 tcaacatgat ggacctgcga gcggcgcaaa tctctgctga gcggcgcgaa caatggcagt
     4261 cggccggctc catgccctgg gatgatgaac ccttgcatcg agaggcgccg attccctggc
     4321 attgggtaga agacagccag acgctctggc gccgccgcct caccatgccg cgccgcgtca
     4381 agacaaccat tctgcatggc ctgcaggata cggtgatcaa gccggaaggc agttgggcct
     4441 tcgtgcagca tgtgctgtcg caggatccgg agtttcccgt tgaactgctg ctcaaaaccg
     4501 gcgaccatcg gctgagcagt ccggaggatt tggacacatt ccggcggctg gtggtgaagg
     4561 aataaccatg ttgatgttaa tccacaaaga atgcggtggt ccggcgctgg aagaatcgcc
     4621 ggtcggcgaa gtctgcgcga ttccggtcga tcggtttccg ttttcctgct tcagctgtct
     4681 ggaagaaatc atcgacgaat cggaattgcg gctcatagaa gatttgggca tctgacccat
     4741 agaggaaggc ggacatttca tgtcatcgtt aggcgcacat aacagcggac cggattcaga
     4801 aggtccggac gttcccgaac cctcccatgc cgaaaaggcc aggacgctgg tgcatctgca
     4861 gcagacgggc ggcctctcca ccctctcacg caagcaaccg ggctggccgt tcgggtcggt
     4921 gatgccgtat ggattggacg atcagggcca gccaagtttt ctgatcagca ccatggcgat
     4981 gcacacgcag aatctgctgg gagatccccg cgcgagcctg ctcgtgacac cgccggagag
     5041 ccaacgcgat ccgctgggcg cagccagagt cacgctcatg ggatcggtca cgaaggtgcc
     5101 taaagatgag gcggcaccgg tgcgagaacg gtatctggcc cgtcatgcca acgcagccta
     5161 ctgggtggac ttcgatgact tcgcgttttt tcgcatggcg ctggcggaca tttattttgt
     5221 cggtgggttt ggttcaatgg gatgggttgc accggctgag tatctggcgg cagcagtcga
     5281 tcctttggca gagaccgctg cggacctcat ccgcgagatg aataccacgc aacaggagac
     5341 actgctgttg cttgcccgcg aatgtggcaa tctcgatgcg cagcaggcga gcatcaccac
     5401 gatggaccga ttggggtttc acctgcgtgt gaaaacaccc gagcgtatgc agggtgggcg
     5461 attcgccttc acgaatccag tgcgcaccgc gcaggaagcc cgcgccggtg ttgccga
//
LOCUS       DAOQEW010000228         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5808_length_5516_cov_7.881157, whole genome shotgun sequence.
ACCESSION   DAOQEW010000228 DAOQEW010000000
VERSION     DAOQEW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_5516_cov_7.881157"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="PLS35_17220"
     CDS             complement(<1..1976)
                     /locus_tag="PLS35_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI20466.1"
                     /translation="MDFSKFFIDRPIFAAVLSIVIFAAGLIAIPILPISEYPEVVPPA
                     VIVRAIYPGANPKVIAETVSTPLEEQINGVENMMYMKSVAGSDGVLQMTITFRPGTDP
                     DAAQVQVQNRVSQALSRLPSEVVSLGVTTQKQSPTFLVMVQLLSPEGKYDALYLRNYA
                     NIKIKDELARLPGVGQVQIFGSGDYAMRIWLNPDKIASRGMTASDVVAAVREQNIQVS
                     AGQLGAEPIAKDTDFLISINAQGRLRTAQEFGNVVLKIGAGGEVVRLSDVARIELGAN
                     DYTLRGQLDNQDAPPIGIFQAPGANALTVRDAVIAKMEELKTRFPPGLTYRSDYDTTV
                     FVRDSIQAVVQTLMEAILLVVLVVILFLQTWRASIIPLIAVPVSVVGTFAALYLFGFS
                     INTLTLFGLVLAIGIVVDDAIVVVENVERNIEEGRTPLQAAHQAMQEVSGPIVAIALV
                     LCAVFVPMAFLSGVTGQFYKQFAVTIAISTVISAINSLTLSPALAAKLLKSHGTPKDA
                     LSRLIERLFGWVFRPFNRFFKASSNGYQGTVSRTLKHRGSVFAVYALLLIVTGMLFQA
                     VPRGFIPTQDKLYLMAGVKLPEGASLDRTDAVLRQVVAISKTVDGIAHVVAMTGLNPL
                     QFTNTPNYGIAFLILKPFDERHRSAKEISEEMNQ"
     assembly_gap    1977..2076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2077..2993)
                     /locus_tag="PLS35_17225"
     CDS             complement(<2077..2993)
                     /locus_tag="PLS35_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20467.1"
                     /translation="MRTIRYTSWLLAVLLTAAGCNNEQASSGGQAPPPPEVGVAQVIS
                     KPVQQWDEYTGRISAIDTVELRPRASGYVQRVAYKEGQDVKQGDLMFLIDPRPYRAAL
                     ENAQAQLARARVAQQLETIRNKRAQALIDDNAISHEELDLRRAAQAQSAADVHAAEAA
                     VATAKLNLSFTEVRAPVSGRASRALVTVGNLATADDTLLTTVVSQDPMYVYFDADENS
                     YLRYKEQERKNERSAQDNAVHVGLANEAGYPHSGRVDFLDTQVNPTLGTVRARAVLPN
                     PDRIFTPGLFARVQFVSGQKAQALLIDDKA"
     gene            3174..3665
                     /locus_tag="PLS35_17230"
     CDS             3174..3665
                     /locus_tag="PLS35_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNI20468.1"
                     /translation="MKRVNVATRMKPKETTCVSPCISTLPRHRLVELLWSFAPAYQRW
                     SESLLVEKGLSPQRLRILGSIHERGPRIMSELKKELGVTATNVTALVDSLENDGLVVR
                     RRHPTDRRATVIELSDKVMRELSPRCTEYKEQVAELFSDLSDQECKAFVKTLEKLWSR
                     LQG"
     gene            complement(3942..4112)
                     /locus_tag="PLS35_17235"
     CDS             complement(3942..4112)
                     /locus_tag="PLS35_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20469.1"
                     /translation="MDEVRGLQFTARRLATQLDLLIQALEDAKPSQEIQELCREGRVV
                     LDYATTVLGGDE"
     gene            complement(4156..4593)
                     /locus_tag="PLS35_17240"
     CDS             complement(4156..4593)
                     /locus_tag="PLS35_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20470.1"
                     /translation="MLPEMLLFATIHGTQALPVSDKVPYESAEASAKLFSEDEIRVVK
                     GIILDTTAVRLKDGVEFMQMEVETERGPVRVHLGPTWYMNEYHERFDVNKGRLVTAVC
                     SPALVDGRHVLVAAELANADGQQRMRLRHPDGTPAWVGGEHVK"
     gene            complement(4790..5083)
                     /locus_tag="PLS35_17245"
     CDS             complement(4790..5083)
                     /locus_tag="PLS35_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20471.1"
                     /translation="MPDLTKNEAIIAALESAGYQEIRLTVVPKRFLFSDGRCHTELEF
                     SRNYFDDTTLIALQGQMGEHILPAIKQQPGKKVYVDARGISIIPSTHGRTDKH"
     gene            complement(5194..>5516)
                     /locus_tag="PLS35_17250"
     CDS             complement(5194..>5516)
                     /locus_tag="PLS35_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20472.1"
                     /translation="YPVLFEAAEKNTELKYTLQFLEKDMAGVSKEVMSFFEQFHISGT
                     HHHTTQDIGNILFLLQTRIRREEDTLFAEFDKLKAAARTIVAVQIPDSRSSILASSHS
                     IHPM"
BASE COUNT         1094 a         1498 c         1664 g         1160 t
ORIGIN      
        1 ttttgattca tctcctcgct gatttccttg gcgctgcggt ggcgctcgtc gaacggcttc
       61 aagatgagaa aggcgatgcc gtaattcggc gtgttcgtaa actgcaacgg attcaggccg
      121 gtcatggcta ccacatgggc gatgccgtcc acggtcttgc tgatcgcgac cacctgccgt
      181 aacaccgcat ccgtacggtc gagcgaggcg ccttccggca gtttcactcc ggccatcaga
      241 tacaacttat cctgggtggg aatgaagccg cgcggtaccg cctggaagag catgcctgtg
      301 acgatcaaga gcaatgcgta gacggcaaag accgaaccac gatgtttcaa cgtgcgcgag
      361 acggtgcctt gatacccgtt ggaactggcc ttgaaaaagc gattgaacgg ccggaacacc
      421 cagccgaata gacgttcgat caagcgcgaa agggcgtcct tcggtgtgcc gtggcttttg
      481 agcagttttg ctgcgagggc cggggaaagg gtcagggaat tgatggccga aatgaccgtc
      541 gaaatggcga tcgtcacggc gaactgtttg tagaactgcc cggtgacgcc gctcagaaag
      601 gccatcggca caaacacggc acacaagacg agcgcgatcg cgacgatcgg gcccgacact
      661 tcctgcatcg cctggtgggc cgcctggagt ggggtgcgtc cttcctcgat attgcgttcg
      721 acgttttcca ccaccacgat cgcgtcatcc accacgatgc cgatggcgag gacaagcccg
      781 aagagcgtga gtgtgttgat ggagaaaccg aagaggtaca gcgcagcgaa cgtgccgacc
      841 accgacaccg gcacggcgat cagcggaatg atcgaggcac gccaggtttg caggaacagg
      901 atgacgacga ggacgacgag cagaatcgct tccatgagcg tctggaccac ggcctggatt
      961 gaatcacgca cgaataccgt cgtgtcgtag tccgagcggt aggtgaggcc gggggggaag
     1021 cgtgtcttga gttcttccat tttggcaatg acggcgtcgc ggacggtcag cgcgtttgcg
     1081 ccgggcgcct ggaagatacc gatgggcggt gcgtcctgat tgtcgagttg tccgcgcaac
     1141 gtgtagtcgt tggcgcccag ttcgatgcgc gccacgtcgg aaagacggac gacttccccg
     1201 cccgcgccga ttttcagcac gacattgccg aactcctggg ccgtgcgcag gcggccttgg
     1261 gcattgatgg aaatgagaaa gtcggtgtct ttggcgatcg gttccgcgcc gagctgcccg
     1321 gccgagacct gaatgttttg ctcccgcacg gcggcgacga cgtcactggc ggtcattccg
     1381 cgcgacgcga tcttgtcagg gttgagccag atacgcattg catagtcgcc gctgccgaaa
     1441 atctgcacct ggccgacacc gggcaaacgc gccagttcat cttttatttt gatgttggcg
     1501 tagttgcgca ggtagagcgc gtcgtatttg ccctccggtg aaaggagctg aaccatcacc
     1561 aggaaggtcg gcgactgttt ctgcgtcgta acgcccaggc tgaccacttc cgacgggagc
     1621 cgtgaaagcg cctgactcac acggttttgc acctgcactt gggctgcatc cggatccgta
     1681 ccgggacgga aggtgatcgt catctgcaac acgccatcgg agccggcgac cgatttcatg
     1741 tacatcatgt tctcgacgcc gttgatctgt tcctccagcg gcgtggacac ggtttcggcg
     1801 atcaccttgg gattggctcc ggggtagatg gcgcgcacga tcaccgccgg cgggacgacc
     1861 tcggggtatt cgctgatcgg aaggatggga atggcgatca agcctgcggc aaagatcacg
     1921 atggacagta ccgcggcgaa gatcggccga tcgatgaaaa atttcgaaaa gtccatnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcct tgtcgtcgat caagagggcc
     2101 tgtgcctttt ggccgctgac gaactgcacg cgagcgaaaa gaccgggggt aaagatgcgg
     2161 tcgggattgg gcagcacggc gcgtgcccgc acagtgccga gggtgggatt cacctgcgtg
     2221 tcgaggaaat cgacccggcc gctatgcgga tagccggctt cgttggcgag gccgacatga
     2281 accgcattgt cctgcgcact gcgttcgttc ttacgttcct gttccttgta gcgcaggtaa
     2341 ctgttttcgt cggcatcgaa atagacgtac atcgggtctt gcgacactac cgtcgtgagc
     2401 agggtatcgt cagcagtcgc caggttgccg acggtcacca acgcccggct cgcccgcccg
     2461 gagaccgggg cgcggacctc cgtgaacgac aggttgagct tggcggtcgc caccgcagcc
     2521 tcggcggcat ggacatccgc cgcgctttgc gcctgcgctg cgcgccgcaa gtcgagttct
     2581 tcgtgcgaga tcgcgttatc gtcgatcaaa gcctgggcgc gcttgttgcg gatggtttcc
     2641 aactgctgcg ctacacgcgc gcgtgccagc tgcgcctggg cgttttccag tgcggcccgg
     2701 tagggacggg gatcgatcag gaacatcaag tcgccctgtt tgacatcctg gccttccttg
     2761 tacgccactc gctgcacgta gccgctggcg cgggggcgta attccaccgt gtcgatcgcg
     2821 ctgatgcggc ccgtgtattc gtcccattgt tggaccggtt tcgaaatcac ttgcgcgacc
     2881 ccgacctcag gcggaggtgg cgcctggccg ccgctgctgg cctgttcgtt gttgcagccg
     2941 gctgcggtca gaagcactgc cagcagccag gatgtatatc ggatagtgcg catggtgtac
     3001 ctgttggatg atcgatggtg aacgatgacg tcagtgcaag agtccggggt tcttcctcac
     3061 ccctgtcgtg cagtatccgg cacggtgtga aacatagtca atatatgaat gatgcaccta
     3121 tgaataattt atgattgaaa ggtgtcgcgt tcttgaagta ccgtatctgt acgatgaaac
     3181 gtgtcaacgt tgccacgcga atgaagccca aggagacgac atgcgtctct ccctgtatct
     3241 cgacgttgcc ccggcaccgg ttggttgagc ttttgtggag ctttgctcct gcctatcagc
     3301 ggtggtcgga gtcgttgctg gtggagaagg ggttatcgcc tcagcggttg cgcatcctcg
     3361 gctccattca tgagcgcggc cccaggatca tgagcgagct gaaaaaagag ctcggcgtga
     3421 cggcgacgaa cgtcacggcg ctcgtcgatt cgctggaaaa cgatggattg gtggtgcgga
     3481 gacgacatcc cacggatcgc cgggccacgg tgatcgagct ctcggacaaa gttatgagag
     3541 agctctcgcc ccgctgtacg gaatataaag agcaagtcgc cgaactgttt tcagacctga
     3601 gcgaccagga gtgtaaagca ttcgtgaaaa cgctcgagaa gctgtggagt cgattgcagg
     3661 ggtaaagcac agtcgtaaga aagagtcttt gcaggtaccg cagcagaatt caggaaggtt
     3721 cgcggtagct tcgactaggc cacttggatt cagccttgcg gtacgggcga gagggcggtc
     3781 gacgcgtcac aagtcgcttg aacaggagta ccagggtggc gagcgacagt attgagaaga
     3841 gacgagcgaa catacgatca cgatatccct ccacgacgag agtgtcaaac acgcacgatc
     3901 cagacgtcat gcttccaagc cgtggaatgt ccctctgagt ctcattcatc tcctcccaga
     3961 acggtagtgg cataatccag taccacccga ccctctcggc acaactcctg aatctcctgg
     4021 gaaggcttcg cgtcttcgag ggcctgaatc agcaggtcca actgtgtggc aagacgacgc
     4081 gcagtgaatt ggagtccacg tacttcatcc atggggctcc ttgaggcgag actgctgact
     4141 gcgaccgatc atccgtcact tgacgtgttc gccgccgacc caagccggcg tgccgtctgg
     4201 gtgccgaaga cgcatgcgtt gctgaccgtc tgcattcgcc agctcggcgg ccactaatac
     4261 gtgtctgccg tctaccagag ccggactaca gacggccgtc acgagtcgac ctttattcac
     4321 gtcaaaacgt tcgtgatatt cattcatgta ccaagtcggt cccagatgca cccggactgg
     4381 accacgttcg gtttctactt ccatctgcat aaactccacc ccatctttca atctgacagc
     4441 ggtggtatcc agtatgatgc ctttgacgac tcgaatctca tcctctgaga agagttttgc
     4501 cgaggcttct gctgattcgt agggtacctt gtcggatacg ggcagagcct gtgtcccatg
     4561 aatcgtggcg aagaggagca tttctggcag catgccgagt ctcctttgtg aatgtatgag
     4621 ttgaccgtag cacccagtcg gcctgcagca aactaggagt gtcccgcgca tgctgccctg
     4681 caccatcgaa ataggaaaag gggagagagg ctcggctgga tcctgccggc atcacaggtt
     4741 agctggagtg gggggatccg ctgcggagcg gaaaggatgg gaggagctac tagtgtttgt
     4801 ccgtccggcc gtgcgtggaa ggaatgatac taattccgcg ggcgtccaca tacaccttct
     4861 ttcccggctg ctgcttgatg gccgggagga tgtgctctcc catctggcct tgcagcgcga
     4921 tgagcgtggt atcgtcaaaa tagttcctgg agaattccag ctctgtgtga cagcgcccat
     4981 cgctaaacag aaagcgtttg gggacgactg tcagcctgat ctcctgatag ccggctgatt
     5041 ccagtgccgc aatgatggcc tcgttcttcg tcaggtctgg catcgtgcct ccctctctcc
     5101 gtctatctct gattccggca cgcgttgatc tgctgatgcg tcggtcgtcg agcatgtgag
     5161 cgcgggcttg gactgcatcg tggaggccgt cgttcacatg ggatggattg agtgagagga
     5221 agccaaaatg gaagatcggg aatcagggat ctgcacagca acgatagtgc gggccgccgc
     5281 cttcagtttg tcgaattccg cgaacagcgt gtcttcctct cggcgtatgc gtgtttgcaa
     5341 cagaaagagg atgttgccaa tgtcctgtgt cgtgtgatgg tgtgtgccac tgatgtggaa
     5401 ctgctcaaaa aagctcatca cctccttcga gacgcctgcc atgtctttct caaggaattg
     5461 cagcgtgtac ttcaactccg tattcttttc ggcggcttcg aataagacgg gataca
//
LOCUS       DAOQEW010000229         5498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5842_length_5498_cov_9.185192, whole genome shotgun sequence.
ACCESSION   DAOQEW010000229 DAOQEW010000000
VERSION     DAOQEW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5842_length_5498_cov_9.185192"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLS35_17255"
     CDS             <1..1083
                     /locus_tag="PLS35_17255"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNI20473.1"
                     /translation="VVEEAKTDDPVILVQDYHFALLPKMVREKLPNATIIMFWHIPWP
                     NSESYGICPWRQEILEGLLGSSIVGFHTREHGNHFLSCIDRILEARIDRDSSTVSYGG
                     RLTAVNPYPISIEWPSRWLENQRPVPDCRVHIRERHAMGPDRLVGIGVDRLDYTKGII
                     ERFLAVERLFELQPEWVGKFSFIQIAAPSRTIIDQYQHFHDQVYALAERINKRFGREG
                     YEPIVLKVKHHEPPDVYEYYRASDLCFVTSLHDGMNLVAKEFIAARDDEQGVLILSQF
                     TGAAQELPEALVVNPYDIDQCAAALHMALSMTPKEQRARMRSMRGLIQEFNVYRWAGR
                     MLMDAARMRRRIRVMKQVGQASGRGR"
     gene            1101..>1561
                     /gene="otsB"
                     /locus_tag="PLS35_17260"
     CDS             1101..>1561
                     /gene="otsB"
                     /locus_tag="PLS35_17260"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNI20474.1"
                     /translation="MFSAQGKRVLDRLASQGGSLFAFDFDGTLARIVQDRHAAGLTES
                     TREALQGLAEVAPTAIISGRSLADLAPRVKGIAAHLIGNHGLEGLHASERVMQQAQDC
                     CSAWLKAVTKDALDLTRAGVVVEDKMYSLTFHYRQAWSPAAARETIFHVVST"
     assembly_gap    1562..1661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1962..2447)
                     /locus_tag="PLS35_17265"
     CDS             complement(1962..2447)
                     /locus_tag="PLS35_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNI20475.1"
                     /translation="MLKEVSGDILLSTAGAIAHGIAPHDNFKQGLALALREQWPGMYK
                     DFRHYCQTYNPKPGGAWSWKGPNSPVIINLFTQEPPATEQDHPGKATLPNVNHALQAL
                     KKELQEHQVKSLALPRLATGVGGLEWEKVYPLLQQALKELHIPVYVYGLYKKGVAAQE
                     A"
     gene            2602..3420
                     /locus_tag="PLS35_17270"
     CDS             2602..3420
                     /locus_tag="PLS35_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNI20476.1"
                     /translation="MERYRIKPGCRPSLKRFDPDDTGSYKKNAAGKAKAKTAAAKHIA
                     TLDQLQERLYANRTRALLIILQGMDTSGKDGTIKHVMSGVNPQGCHVASFKAPSEKEL
                     EQDFLWRIHQEVPAKGYIGIFNRSQYEDVLITRVHGLVSKKIVTQRFNQINEFEELLV
                     ENGTVIIKLFLHISKEEQKARLTERICDPEKRWKFSEGDLEERKLWDDYMTAFEDLMA
                     ATSTERAPWYIVPANRKWYRNLVVAELVMQALAAMKLSTPPAPKGVNFDGLTID"
     gene            3517..4395
                     /locus_tag="PLS35_17275"
     CDS             3517..4395
                     /locus_tag="PLS35_17275"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNI20477.1"
                     /translation="MRMKAAFLIGLVSSLTALPAYAGDFEGVIHMKNSHVDTGTSRAM
                     DWYLKGDRGRVETIREDGQAHVMLFNGKTRTMQMGMPGQKSYMEISLEGDRGEHLNEL
                     LEAQHVERTGKTEKIAGYTCEVWRITDKEQHRPKSELCVAKGFGKAATFWIDPKEARR
                     SSQPGWVKQLAEEGGFGLRSIQYDEAGKESGRMEVVSIEKKTLDASLFAFPADWAKHD
                     LSGFQERMKAMREQKREGGADVSKMVEEMKKRKAARGKPGEAPGGEEQPPDMKELMKQ
                     FGEAMKKQQSQPQGGQ"
BASE COUNT         1173 a         1478 c         1644 g         1103 t
ORIGIN      
        1 gtggtcgaag aggccaagac cgatgatccc gtcatcctgg tgcaggacta tcactttgcg
       61 ttattgccca agatggtacg ggagaagttg ccgaacgcca cgatcatcat gttctggcat
      121 attccctggc ccaactccga gagttacggc atttgcccct ggcgacagga aattttggag
      181 ggattactgg gcagcagcat cgtcgggttt catacccgcg agcacgggaa ccatttcctg
      241 tcctgtatcg atcgcatttt agaggcacgc atcgatcgcg actcgtccac cgtgtcgtat
      301 ggtgggcggc tgacagcggt gaacccctac cccatttcca ttgagtggcc gtcgcgctgg
      361 ctggaaaacc aacgccccgt tcccgactgt cgggtgcaca ttcgcgagcg acatgccatg
      421 gggcctgatc ggctggtcgg catcggagtc gaccgcctgg attacacgaa gggcatcatc
      481 gaacggtttc tcgcggtcga acggttgttt gaactgcagc cggaatgggt cggcaagttt
      541 tcgttcattc agattgcggc gccgagccgg acgatcatcg atcagtatca acattttcac
      601 gatcaggtct atgccctcgc ggaacgcatc aacaaacggt ttggccgtga agggtatgag
      661 ccgatcgtgc tgaaggtgaa gcatcacgag ccgccggatg tgtatgagta ctatcgcgcg
      721 tccgacctgt gcttcgtgac cagtttgcat gacggcatga acctggtggc caaagaattc
      781 atcgccgctc gtgacgacga acagggcgtc ctcatcctca gccagtttac cggcgccgcc
      841 caggagttgc ccgaagcgct ggtggtcaat ccctacgaca tcgaccaatg cgctgcggcc
      901 ttgcacatgg cactgtcgat gacgccgaaa gaacagcgtg cacgtatgcg gagcatgcgc
      961 gggttgattc aggaatttaa tgtctatcga tgggcgggcc gcatgctcat ggatgcggcc
     1021 agaatgcgcc gtcgcattcg cgtcatgaag caggtggggc aggcctcggg gcgggggcga
     1081 tagtcacgcc gatgcaggac gtgttcagtg ctcaaggcaa acgtgtgctc gaccggctgg
     1141 ccagccaggg cggctcgctt tttgcctttg atttcgacgg cacgctggcg cgcatcgttc
     1201 aggatcggca tgccgcaggt ctgaccgaat cgacgcgtga agccttgcaa ggtctggccg
     1261 aggtcgcgcc gaccgcgatc atttccggac gttccctggc ggacctcgct ccccgtgtga
     1321 agggcattgc tgcccatctc atcggcaatc atggactgga ggggttgcac gcgtcggagc
     1381 gtgtcatgca gcaggcgcag gactgttgca gcgcatggtt gaaggccgtg accaaggatg
     1441 cccttgacct gacgcgagcc ggcgtcgtgg tggaagataa gatgtactcc ctgacctttc
     1501 actatcgtca ggcatggtcg ccagccgccg cccgtgaaac catttttcat gtcgtgtcca
     1561 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatctcaca ggcgcccgcg
     1681 ccagggcggt ctcagcctaa caggatgcgg aaaggccctc gcgcggcgtt ctcgcaggac
     1741 actgccgcct cactttctcg gcggcgttta caagcgtgac gcgctgtatc agcgcgtcgt
     1801 gaccctccga gactcaccgt acggcagggg tacgactcgc ctcttccttc gctgcggcct
     1861 tgctggacga tcttttgcgc atcctgcggg aggtgccgac gccagcaagg cacttgggtt
     1921 gctcatgtgc tctcatgcaa aaacggttgt gccgtaggcc gctaggcttc ttgcgcggcg
     1981 acccctttct tgtacagtcc atacacgtag acgggaatgt ggagctcttt cagggcctgc
     2041 tggagcaagg gatacacctt ttcccattcc aaccccccca ctcccgtggc cagacgaggg
     2101 agcgccaggc tcttcacctg atgctcctgc agttcctttt tcagcgcctg cagcgcatga
     2161 ttgacgttgg ggagtgtcgc cttgccagga tgatcctgtt cggtggccgg cggctcctgc
     2221 gtaaataaat tgatgatgac gggagagttc gggcctttcc acgaccaggc gccgcccggt
     2281 ttgggattgt aggtctggca gtaatggcga aaatctttgt acatgcccgg ccactgctcg
     2341 cgaagtgcca acgccaaacc ctgcttgaaa ttgtcgtggg gagcgatacc atgcgcgatc
     2401 gcgccggcgg tggataacag aatgtctccg cttacttcct tgagcatgac cgatgcctcc
     2461 ttgattgagt aaaagatctg tttatcacat aagactgtgc cctcttcaat caatggattc
     2521 atagaaataa atttttccac gctataaggg cgactacttt gatcgccgac cacaggacgc
     2581 tgccatagca ggaggaacga catggagagg tatcgaatca agcccggttg ccgtccatca
     2641 ctcaaacggt ttgatcctga cgataccggg tcgtacaaaa agaacgcggc tgggaaggcc
     2701 aaggccaaaa ccgccgcggc caaacacatt gccacgctcg atcaactgca ggaacggctg
     2761 tacgccaacc gcacacgggc gttgctgatc attctgcaag gcatggatac gagcggaaag
     2821 gacggcacga tcaagcatgt catgtccggt gtgaacccgc agggctgtca cgtggcttcc
     2881 tttaaagcgc cgagcgagaa agagctcgag caggattttt tgtggcggat tcatcaagaa
     2941 gtgccagcca agggctacat tggcattttt aaccggtcgc agtatgaaga cgtgttgatc
     3001 acgcgtgtgc atggtctggt gtcgaagaag atcgtcacgc agcgatttaa tcagatcaat
     3061 gagttcgaag agctactggt cgagaacggc acggtgatca tcaagctgtt cctgcacatt
     3121 tccaaagagg agcagaaagc gcgtctcacc gaacgcatct gtgatcccga gaagcgctgg
     3181 aaattcagcg agggagattt ggaagagcgg aagctgtggg acgactacat gacagcattt
     3241 gaagacctca tggcggcgac cagcaccgag cgggctccct ggtacatcgt gcctgcaaat
     3301 cggaaatggt atcggaatct ggtcgtcgcc gagctcgtga tgcaggcgct tgccgcgatg
     3361 aaactttcca cgcctccggc ccccaaaggc gtgaacttcg atgggctcac gatcgactaa
     3421 tggccgtacg cgccagagtc atctggcatc gtgtgtgggg ctcggctaca ctggcaagcc
     3481 tgaatcgtgt gtctacctta accgggagcg tggagtatgc gaatgaaagc ggcctttttg
     3541 atcggcctcg tctcgagcct cactgccctc cccgcctacg cgggtgattt cgagggtgtg
     3601 atccatatga aaaacagtca tgtggatacc ggcacttcgc gcgccatgga ttggtatctc
     3661 aagggcgata gggggcgtgt ggaaacgatc agggaggatg gccaggccca tgtcatgctg
     3721 tttaatggca agaccaggac gatgcagatg ggcatgccgg ggcagaagtc ttatatggag
     3781 atcagcctgg agggagatcg gggtgaacat ctgaacgaat tgctcgaagc gcaacatgtc
     3841 gagcgcaccg gcaagaccga gaagatcgcc ggatatacct gcgaggtgtg gcgcattacc
     3901 gacaaggaac aacatcgccc gaagagtgag ctctgtgtgg caaaaggttt cggaaaggcc
     3961 gcgacgtttt ggatcgaccc caaggaggcc cggcggtcct ctcagcctgg ctgggtgaag
     4021 cagttggctg aagaaggagg gttcgggctt cggagtatcc agtacgatga agcgggcaag
     4081 gaatccggcc gcatggaagt ggtcagtatt gagaagaaaa ccttggacgc gagcttattt
     4141 gcgtttcccg ccgactgggc caagcacgac ctctccggct ttcaagaacg gatgaaggcc
     4201 atgcgggagc agaaaagaga aggcggggca gacgtctcga agatggtcga ggagatgaag
     4261 aagcgaaagg ctgcacgtgg gaaacccggc gaagctcctg gcggcgagga gcagcctccg
     4321 gatatgaaag agctgatgaa gcaattcggc gaagcgatga agaaacagca gtcgcaaccg
     4381 cagggcgggc aataagcggc atgcctgggt cgaaaagagt ggcacatcaa atcgcacagc
     4441 gaggtgaatc cgatccgtct atggccggct caggaattcg gcgtgagctc cgatggcggt
     4501 ggacctggga attccccttg ttgacaagtt tttttgaggg gcgtagatag gggctgcggc
     4561 ccatttcgct tgtcgcaagt ggaagaccgg acagaccgcg cggctgccat ctagatcttc
     4621 cacacattcc cgcaacagaa gggagggtag acgatgaccg atacctctcc acctggcctg
     4681 tacggcctgc cgaactgaag gcaggtttct cgtcggtttt ttttgccaac cggttctcat
     4741 cgcaaatgcc acaaggcatt ccacgacgcg ctcatgcgcg tcccggcgtc atgccaccga
     4801 atggagggcg aggttgacga tctttgacgc ggtcatcagg ccggccgtga tcgcgagctc
     4861 atcggcgaga tggcttctgc tattgatcaa ctcggcgggt cttctacggg ccgctacata
     4921 gacagagcga tcggcgtcct aagcgccaca cacgggctcc cgtcacacac gggagcccgt
     4981 gttgtatcgg gactctcccg gccaccggga acatgaggaa aaatgactag ggccgatttt
     5041 cctgttgaca gagttttcta acgagcgtaa gagggaaatt gtaagcttca agggccgtgt
     5101 taaccgtgac gaggcgatgg acagtgcgcg cctgttcggg agacctcgcc actcctcaac
     5161 cggggaggtc cacgcatgga agaacttacg ccgccggact aaaacggccc gccgagttga
     5221 gggcgggctg atcgtcggtc gtgcagaccg atccttccct ggcttgtgtc tgatggcact
     5281 atccgtttaa ggataacagc cagcgcaaag atgaaggatt gaagccaagc acgaggcaca
     5341 ccagaatcct gggaacatgc ttcccccgac gaacatcctg atcatccatc ttctcgaacg
     5401 ggtctggtct gtcggacaac ctatttgact gtaggcgtcc caagcgccaa ccacgggcga
     5461 cctcccaccg cagtgtaggg tcgcccgtgg cattttaa
//
LOCUS       DAOQEW010000230         5485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5871_length_5485_cov_4.960405, whole genome shotgun sequence.
ACCESSION   DAOQEW010000230 DAOQEW010000000
VERSION     DAOQEW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_5485_cov_4.960405"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3409)
                     /locus_tag="PLS35_17280"
     CDS             complement(<1..3409)
                     /locus_tag="PLS35_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20478.1"
                     /translation="MPGEDVQGAPSRPHVCTFSVVAKTAAIVSLSLLWWGCAKPKMPE
                     SQQVVKPACERTITAEVVALDQVYYYNRFGAFNPAGIMFALRRDVVHADEEDLLNGDL
                     DNRSDEQEKAITLQHGPADLGLAGNVKLRSDKRPRPLVLRANEGDCLEVTFTNLLSPE
                     TNGQEVMLDPEKRLSRPDKRVTAVSDSDEPSTRHASMHVNGLSLVDSIDSQGANVGRN
                     GWHDQSGNKTDGRCSSNRSDFGKDLCALAAPGETRTYRWYAKKEGTFFLYSMGAPAGG
                     EGDGGQVGLGLFGAINVQPRGAKWYRSQVTEKDLKAASYKQETKFGQPVIDYEATYKN
                     EPHAGDPILNMLKCDPRDNRQCELVHSDLNAVIDIDRENGCEKMGEGNACGKPYREFT
                     VIFHDEITAVQAFDEIREEGSPLSSLRDGMGINYGASGVGSMVVANRNKLGPARDCVE
                     CKLEEFFLTSWVNGDPAMVVGYERGKGGSYKKDGNGRFVMQARYPDDPSNVHHSYLGD
                     PVRFRNMHAGPKETHVFHLHAHQWVEDKHDPLSHYLDSQTISPGTTFSYEVHYGGSGN
                     RNLGPGDSIFHCHLYPHFAQGMWELWRTHDVFENGGYDRRLKDHDIAGGTPTPAIVPL
                     PRTPLPPMPTPTFKGYPFYIAGEIGHRPPQPPLDLEGGDDAGTLRRHVVAGGTVHDAL
                     DAQGPKEMKQAPPMNHDLFDKGRQQSAQIAARVGTQNTDKDLVGLARELVTAKLDVKA
                     TAGTTDEKAAMKFHAGMSQVNGASAVRPGDPGWTYSPYNWEAPGYVTCLSNGLCDDPH
                     GTRVLFHVNGGGNIAEAKRGEGESGAPFSNPCPKQFIDEKGGVHDVKTREYRAVYIQM
                     NMTVNKSGWHDPQARFPILEQDVKETLDGTRPTEPLFFRAQSGECVVFHATNLIPSNL
                     NLDDFQVYTPTDTIGQHIHLVKFDVTSSDGSGNGWNYEDGTLAADEVRERILAYNKTN
                     PAQRLKPRTHRLFLRKGQLGATENGAMAGDPRGLCSDITGQAADDHDPVGHPWCGAQT
                     TTQRWWADPVLNGKPGDKGTKDRTMRTVFTHDHFGPSSHQHHGFYAALVVEPRNSKWY
                     FPDGQLMGGTDSKGVPEAVNGRNDGGPTSYAANVVVRTQGKACITPDK"
     gene            complement(3498..5054)
                     /locus_tag="PLS35_17285"
     CDS             complement(3498..5054)
                     /locus_tag="PLS35_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20479.1"
                     /translation="MRVPSTVTVWMIGVLVLLGIAPPRHALSQAVIPFHIVGHIQRLT
                     LDSPADPLSGAKLTVNGVEVVLPKNLVIQLPAAYFTAQQLFDKAQGVSKKYGESGLAL
                     SDKFPPLAAFEADVSGNIVNGLYIAGLVTISQQSLNTGAGFIHHIDTATGMMCVGGSP
                     TAAACAGNDTRIRLNDPALDASDPFAGDGRYGKPNPAPSPVGLDDPNSRYPDPRFTVD
                     QGNPTVHALTGYPMCVPRATNDAQCPSQNRPAELTFVMDSVDLVPPVKFGNNAIKACP
                     SCDANKQAPVRVGDYITFSGTRARDPLAGDFLSVHTLVANVGIYTKPGGRAYVSLEES
                     LLGTRGPVVDCGAAAECQDRLKVEGFTTDPSRRVSVYAVDVVPGGVPKVRLLHSTEKD
                     QAVFGRFRYVPPLTAATLFDFNGNLKGATRELMVRIDDPAPLSDGSDVPSAPKAAHGL
                     TAGIYVAPVGEYIFPEPTGVQGGAQPALNFQCLAFLANGWALPDSGLPNIPRLTPWPG
                     VATPTFSCTQ"
     gene            5275..>5485
                     /locus_tag="PLS35_17290"
     CDS             5275..>5485
                     /locus_tag="PLS35_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20480.1"
                     /translation="MTILIPEQMRFSRMHNALSGAVCEQCFRKMNSFNCGAEDAPSAE
                     DGNGFLSIWIQGSIGMDTEWIQAVRI"
BASE COUNT         1133 a         1654 c         1519 g         1179 t
ORIGIN      
        1 ccttgtccgg tgtaatgcag gccttgccct gagtgcgtac gaccacgttc gcggcatagg
       61 aggtcggtcc accgtcattg cgcccgttca ccgcttcagg cacccccttg gaatccgtcc
      121 cccccatcag ttgtccatcc gggaaatacc acttggaatt tctcggctcg accaccagcg
      181 cggcataaaa cccgtggtgc tgatgcgaac tcgggccaaa atggtcgtgg gtgaacaccg
      241 tgcgcatcgt gcgatcttta gtgcccttgt cgccaggctt tccgttcagc accggatccg
      301 cccaccatcg ttgcgtggtg gtttgcgcgc cacaccaggg atgaccgacg ggatcgtgat
      361 catctgccgc ctgtcccgtg atatcgctac acaacccgcg cggatcccct gccatggcgc
      421 cgttctcggt cgcgccaagt tgtcccttcc gaaggaagag acggtgggtg cgaggcttga
      481 gccgttgcgc gggattcgtc ttattgtagg ccagaattcg ctcccgcacc tcatcggcgg
      541 ctaacgtgcc atcttcatag ttccatccat tgcctgatcc atccgatgag gtgacgtcga
      601 atttcaccag atggatgtgc tgaccgatcg tatcggtcgg cgtatagact tgaaaatcat
      661 ccaggttgag attgctcgga atcaggttgg tcgcgtgaaa gaccacgcac tcccctgact
      721 gcgcccggaa gaacagcggc tccgtggggc gagtcccgtc cagcgtctct ttcacgtctt
      781 gttccaagat gggaaaacgg gcttgcggat catgccagcc cgatttgttg accgtcatat
      841 tcatctggat atagacggcg cgatactcgc gagtcttcac gtcatgcacg ccacccttct
      901 catcgatgaa ttgcttggga caggggtttg aaaacggtgc accggattcc ccctctccgc
      961 gtttggcctc cgcaatgttc ccacctccgt tcacatggaa caacacgcgc gtcccgtgag
     1021 ggtcgtcgca gagtccgtta gagagacagg tgacataccc aggcgcttcc cagttatacg
     1081 gactgtaggt ccaccccgga tctcccgggc gaacggcaga agcaccgttt acctgagaca
     1141 ttccagcatg aaacttcatt gccgcctttt cgtctgtcgt gccggcggtc gccttcacat
     1201 ccagtttcgc cgtgaccaat tcgcgcgcca acccgactaa atctttgtcg gtattctggg
     1261 tacctacacg cgcggcaatc tgtgccgact gctgccgccc cttatcaaac aagtcatggt
     1321 tcatcggcgg agcctgcttc atctccttcg gcccctgcgc atccagcgcg tcgtgcaccg
     1381 tcccccctgc caccacgtgt cggcgaagcg tgccggcatc gtccccgcct tccagatcga
     1441 gcggcggctg cggaggtcga tggccgattt ctccggcgat atagaacgga tagcccttga
     1501 acgtcggagt cggcataggg ggcagcgggg tacgaggcaa tggcacaatg gccggtgtgg
     1561 gagtaccccc ggcaatatcg tggtccttca accggcggtc gtagcctcca ttttcaaaca
     1621 catcgtgcgt ccgccagagt tcccacatcc cctgcgcaaa atggggatag aggtgacaat
     1681 ggaaaatgga atcgcccggt ccgagattgc ggttgccgga gccgccgtaa tgcacctcgt
     1741 acgaaaaggt cgtgcctggc gagatcgtct gtgagtcgag ataatgggag aggggatcgt
     1801 gcttatcctc gacccattga tgcgcatgca aatggaacac atgggtctcc ttgggacccg
     1861 catgcatgtt ccggaatcgg acgggatcgc cgagatagga atgatgcaca ttggagggat
     1921 cgtcgggata ccgcgcctgc attacgaatc gtccgttccc gtccttcttg taactgccgc
     1981 ctttcccccg ctcatacccg accaccatcg ccggatcgcc attgacccag gaggtgagga
     2041 agaattcttc gagtttgcat tccacacaat ctcgcgccgg gccgagcttg ttgcggttgg
     2101 ccacaaccat cgaccctacg ccagaggccc cgtaattgat gcccatgccg tcgcgcagtg
     2161 aactcagcgg acttccttcc tcccggattt catcgaacgc ctggacggcg gtgatctcat
     2221 catggaaaat cacggtaaat tcccggtacg gttttccaca agcattacct tcgcccattt
     2281 tctcgcagcc gttttcccgg tcgatatcga tcacggcatt caggtccgaa tggacaagct
     2341 cgcactgccg gttgtctcgc ggatcacact tgagcatatt caaaatcggg tcaccggcat
     2401 gtggctcgtt cttgtacgta gcctcataat caatgaccgg ctgaccgaat ttcgtctctt
     2461 gcttatagct ggcggctttc aagtccttct cagtcacctg ggagcgatac cactttgcgc
     2521 cacgaggctg tacattgatc gccccgaaca ggcctaatcc gacttgcccg ccgtcgccct
     2581 cgccgcccgc aggtgccccc atggaataga gaaaaaacgt cccttccttt ttggcgtacc
     2641 acctgtacgt tctggtttca ccgggtgccg ccagcgcgca cagatctttg ccaaaatcac
     2701 ttcggttgga tgaacaccgg ccatcggtct tgttcccgct ttggtcatgc catccattgc
     2761 gaccgacatt cgcgccctga gagtcgatgg aatccaccaa actcaatccg ttcacatgca
     2821 tggaggcatg tctggtcgac ggttcatcgc tgtcactaac ggcggtcacg cgcttgtccg
     2881 gccgggagag ccgcttttcc ggatcaagca tgacttcctg gccgttcgtc tcgggagaaa
     2941 gcaggttcgt gaaggtcacc tccagacagt ctccttcatt ggcccgaagc accagcgggc
     3001 gaggccgctt atcggaacgc aatttcacat tcccagccaa gccgagatcg gcaggcccat
     3061 gctgaagggt aattgccttc tcttgttcgt ccgaccgatt gtccaggtcg ccattgagaa
     3121 ggtcttcctc atcggcatgc acaacatccc gtctcagcgc gaacatgatg ccggccggat
     3181 taaatgctcc gaaccggttg tagtaataca cctggtccaa ggccacgact tcagccgtga
     3241 tagtcctctc acaggccggt ttcaccacct gctgggattc cggcattttc ggcttggcgc
     3301 acccccacca gagcagggac agactgacaa tcgctgcggt tttggccact acgctgaacg
     3361 tacatacgtg cggtcttgat ggagcgcctt gaacatcttc gccaggcatg tccgcactca
     3421 attgggtaga cgctggaaca aattcatcat ctcgcatggt caaggtctcc actgggctca
     3481 ttcgtgacaa tcacacctta ttgcgtgcag gaaaacgtcg gggttgcgac gcccggccaa
     3541 ggtgtcaggc gaggaatatt gggcaacccg ctatcgggaa gcgcccatcc gttggcgaga
     3601 aatgccagac attggaaatt cagggctggt tgagccccgc cttgcacacc agttggttcc
     3661 gggaaaatat attcgccgac cggtgcgaca tagatgccgg cggtcagacc atgggcagcc
     3721 ttgggtgcgc tcgggacatc gctgccgtcc gatagcggcg cggggtcgtc aatgcgcacc
     3781 atcagctcgc gcgtggctcc cttcaaattt ccgttgaaat caaagagcgt agcggcggtc
     3841 aatggtggaa cgtagcggaa gcgaccaaag acggcttgat ccttttccgt gctgtgaagt
     3901 aaccggacct taggaactcc gcctggcacc acatctaccg catagacgct gacacgccga
     3961 cttggatcag tcgtgaagcc ttcgactttc aagcggtcct gacattcagc agccgcgccg
     4021 cagtccacga ccggccccct cgttcccagc agcgactcct ccaggctcac gtacgcgcgc
     4081 ccaccgggct tggtgtagat accgacgttc gcgaccagcg tatgcacgga cagaaaatct
     4141 cctgccagcg gatcacgagc cctcgtcccc gaaaacgtga tgtagtcgcc aacccgcacc
     4201 ggggcttgct tattcgcatc gcacgacggg caggccttga tcgcattgtt tccaaacttg
     4261 accggcggca ccagatcgac ggagtccatg acgaaggtga gctccgccgg tcgattctga
     4321 ctcgggcatt gcgcgtcatt ggtggcgcgc ggcacgcaca tgggatagcc ggtcagcgca
     4381 tgcaccgtgg gattgccttg atcgacggta aaacgtggat cagggtagcg agagttcgga
     4441 tcatccagac caaccggtga gggcgccgga ttcggcttgc cgtaacgacc atctcctgca
     4501 aagggatccg aggcatccaa ggcgggatca ttcaggcgaa tgcgcgtatc gttgccggcg
     4561 caggccgctg ctgtcggact cccccccaca cacatcatgc cggtcgccgt atcgatgtga
     4621 tggatgaaac cggcgccggt attcaacgat tgttgcgaga tcgtgactag cccggcgata
     4681 tagagcccat tgacaatatt cccgctgacg tccgcttcaa aagccgccaa tgggggaaac
     4741 ttgtcgctca gtgcaagccc ggattcgcca tatttttttg agacgccctg ggccttatcg
     4801 aagagttgct gcgcagtgaa gtacgcggcg ggcaactgaa tcacgagatt cttcggcagc
     4861 accacctcca cgccattcac cgtcagctta gcgccggaca ggggatccgc cgggctgtcg
     4921 agcgtgagcc gctggatgtg tcccacgatg tgaaagggaa tcaccgcctg cgacaatgca
     4981 tggcgtggcg gagctatacc caataagaca agtacaccga tcatccatac ggtgacagtg
     5041 gacggtacac gcatcgagaa tcctcctcct ggaaacagac ggccacctaa cttcaatgta
     5101 ttgacatggg atcaggcccg gaagcagcaa cccgcacgat gggagtacgt aggggtatga
     5161 ctactgagtc cgcatgaggc cgggcacgag ataccagatt gcacaatact tgccaagcta
     5221 tttcctgcct gacccaacga ataaatacat acggcgaggc tcacggatgg aagcgtgacc
     5281 atcctcatcc ccgagcaaat gcggttttca cgtatgcaca atgctctgag tggcgctgtg
     5341 tgcgagcaat gctttagaaa gatgaatagc ttcaattgtg gcgcagagga tgcgccgtct
     5401 gcagaggatg ggaatggttt cttatcgatt tggatacaag gcagtattgg aatggataca
     5461 gaatggattc aggcggtgcg tatca
//
LOCUS       DAOQEW010000231         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5906_length_5462_cov_9.616053, whole genome shotgun sequence.
ACCESSION   DAOQEW010000231 DAOQEW010000000
VERSION     DAOQEW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_5462_cov_9.616053"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLS35_17295"
     CDS             <1..267
                     /locus_tag="PLS35_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20481.1"
                     /translation="AMQIGVELSHSEASMVFCDAGGTHAFPPDKLVSACFALTRPFSV
                     AGSVVSRSEHLYSRPVRGSFHPDSSLTVMIFSCPAPDLAPEYRC"
     gene            complement(275..1498)
                     /locus_tag="PLS35_17300"
     CDS             complement(275..1498)
                     /locus_tag="PLS35_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HNI20482.1"
                     /translation="MQSIQALRERRSAIAQSIHKLLDDNPGDKWTPSLQEKYDQGMSE
                     IENISAEATRVQNVLDLISENFENDAASTVVERTARDKKRPSNVDAFWNLMKKGEKLV
                     TPDEWGVLRNTMSVGTSAQGGYTVPTEVSASLADALKEYGGMRSVSEVFRTTQGNDIN
                     FPTSDGTSETGELIGENTTATGADPSFGVVTLKTYKFSSKVVACPFELLQDSAIDMEA
                     AIMSRLVTRLGRITNTYFTTGTGSGQPNGIVTAATSGKVGTTGQTVTVIYDDLVDLVH
                     SVDPAYRNLGRCKFMMNDSSLKVIRKIKDSSGRPIFMPGYDGLGGSFPDTLLGYQIQI
                     NQDIAVMAANAKSILFGDFTFYKIRDAMDIQMFRFDDSAYIKLGQIGFLAWMRSGGNF
                     VDVGGGVKYYQNSAT"
     gene            complement(1571..2314)
                     /locus_tag="PLS35_17305"
     CDS             complement(1571..2314)
                     /locus_tag="PLS35_17305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HNI20483.1"
                     /translation="MKIKSPLNKLLADNRGKGLFRVDNVSQDEATVWLYDMIVSDSFF
                     EGISAIDFAKQMTAIDAKTIHLRIDSPGGEIFAAQAMAQIIREHAAHTIAHIDGHAAS
                     AASWVALAADEVVISPGGMIMIHNSQTISYGDARDLKDTAALLEKVDGILVATYVEAT
                     GQDAQQITDWMDAETWFSAEEALLYKFADRIARNDDVALENAIQWNLTAWSKAPSKSP
                     ANQDGNRNDQVSTLDHLRRRLRLAEKQTA"
     gene            complement(2311..>2561)
                     /locus_tag="PLS35_17310"
     CDS             complement(2311..>2561)
                     /locus_tag="PLS35_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20484.1"
                     /translation="LLRDGIHFAEFTTAGLERGDYKARNEGYRIALGRAGEPGWMTPN
                     EVRRLENLPPLPDGDTRANGESSAGKKDNEQDNAQDNA"
     assembly_gap    2562..2661
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2662..3803)
                     /locus_tag="PLS35_17315"
     CDS             complement(<2662..3803)
                     /locus_tag="PLS35_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNI20485.1"
                     /translation="MSAAPTWYNAERVAIPGSVILTAWKAQREAARKLSNATTYTTSS
                     GGRDSQLYEWLTGSGSLSTAGPAVNERTALAICAVYACIGLIGGAISSLPLPIYRRGG
                     DSRQRVDHPLSDLLNVEPTPACSAAVWREYLIWALLLHGDGFARIHRKGGRRSLLPDV
                     VRLEPLHPLDVSVSINGDRLAYQLIDSAVDGRMITLDQDDMLHVPGLGFDGCRGASPL
                     RHAARNSMGLSLAADEYSARFFSNGARPDFVITTPGRMDAAAQALFRESWMARYAGLG
                     NSHIPAILTGGGEVKALSLNPEDAQLIETRNYQAADIARFYGVPPHMIGLTDKATSWG
                     SGIEQQGIGFVKYTLSRHLVKIEQEINRKLLRDGIHFAEFTTAGLER"
     gene            complement(3800..>5462)
                     /locus_tag="PLS35_17320"
     CDS             complement(3800..>5462)
                     /locus_tag="PLS35_17320"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HNI20486.1"
                     /translation="EGRIPACKWHRLACQRHLDDLSRAALDPAWPYRFNPQQTDSAGK
                     AYHPAERICRFAELMPHIKGDWAARRQLIKLETWQIFILCSIFGWVHCETGKRRFRVA
                     DLFIPRKNAKSTLAAVIGLYMLAVDGEFGSEIYSGATTQDQAMEVFRPAMLMARATPR
                     YLQAYGVTVNASNISVVSTNSKFEPVIGNPGDGASPSCALIDEFHEHKTSALYDTMQT
                     GMGARSQPLIVVITTAGSDLSCPCYLHQVELQKILESIVENDQRFGIIFGVDDGDDWT
                     SEDSLRKANPNYGVSIDGDFLKMQQRDAISDPRKQNTFKTKHLNTWVAAASPWLNLHA
                     LQQAGDPAITLESMRGKPCVIGLDLASKVDIASAIWLFSEERDGEEHYTAISRNYLPQ
                     TAVDKPENSHYQAWVSGGHITPTPGNMISLRQIQDDVIDSSETVLIREISLDAWGSRE
                     IAPNLQEEGFEVVDIPMQTRHLSEPMKKIAALIDAGRFHHDGNPAYVWMLSNVEVWSD
                     RNENIFPRKLRSQNKIDAAVATIVAMSRAMVPSEEKSPCLELVVL"
BASE COUNT          987 a         1397 c         1696 g         1282 t
ORIGIN      
        1 gcgatgcaga tcggtgttga attgagccat tctgaagcgt cgatggtgtt ttgcgacgcg
       61 ggagggaccc atgcgttccc accagacaaa ttggtgtccg cctgttttgc actgaccagg
      121 ccgttttccg tcgcggggtc cgtagtcagc aggtcggagc atttgtattc ccgtccggtg
      181 cgcgggtctt tccatccgga cagtagtctg accgtcatga ttttctcctg tccggcgccc
      241 gatctggcgc cggaatatcg atgttgaaat tcaattaggt cgcggagttc tgataatact
      301 tgactccgcc gccgacgtcg acgaagttcc cgccacttcg catccaggcg agaaatccga
      361 tctgtccgag tttgatgtag gcgctgtcgt caaaacggaa catctggatg tccatagcgt
      421 cacggatttt gtagaacgtg aaatcgccga acaagatcga tttggcattg gcagccatca
      481 cggcgatgtc ctgatttatc tggatttgat agcccaacag ggtgtccggg aaggatccac
      541 cgaggccatc gtatccaggc atgaagatcg gtcgcccgct ggaatccttg atcttgcgga
      601 ttaccttcag cgaggaatcg ttcatcatga atttgcaccg cccgagattt cgataggcag
      661 gatcgacgga atgtaccagg tccaccagat cgtcgtagat gaccgtgacg gtctgtccgg
      721 ttgttccgac ctttcctgac gttgctgccg tgacaattcc attgggctga ccgctgccgg
      781 tgccggtggt gaaataggta ttggtgatgc gtcccagtcg ggtgacgagg cgcgacatga
      841 tggcagcttc catgtcgatc gccgaatcct gcagcagttc gaatgggcat gccacgactt
      901 tcgagctgaa tttgtaggtt ttgagcgtga ccacaccgaa gctcgggtcg gcaccggtgg
      961 cggtggtgtt ctctccgatc agctcgccgg tttccgaagt gccgtcagac gtcggaaaat
     1021 tgatgtcgtt cccctgcgtg gtgcggaaga cttcggatac agagcgcatg cctccgtatt
     1081 ccttgagcgc atcggccaag gaggcggaaa cctcggtggg aacggtatat ccgccttgtg
     1141 ctgaggtgcc tacggacatg gtgttacgca gcacccccca ttcgtccggc gtgaccagct
     1201 tctcgccttt tttcatgaga ttccagaagg cgtcgacgtt ggacgggcgc tttttgtcac
     1261 gcgcggtgcg ctcgacgacg gtactcgccg cgtcattctc gaagttctcg gagatgaggt
     1321 cgagaacatt ttggacgcgc gtggcttcgg cggagatgtt ttcgatttct gacattccct
     1381 ggtcgtattt ttcctgcagg gatggggtcc acttgtcgcc cgggttgtcg tcgagcagtt
     1441 tgtgaatgga ttgtgcgatc gcggaacggc gctcccgcag ggcttgaatg gattgcatgg
     1501 tgttctctct cctaaataga ggatgtaaaa aaaccgcact ctgccggttg atcagcagac
     1561 gcgggagcgt ctaggccgtc tgtttctctg ccaggcgtaa tcgccggcgg aggtgatcga
     1621 gggttgagac ctgatcgttt ctgtttccat cttggtttgc tggcgatttt gatggcgctt
     1681 ttgaccaggc tgtgagattc cactggatgg cgttctcgag ggcgacatca tcattcctgg
     1741 cgatccgatc ggcaaacttg tacagcagtg cttcttccgc cgaaaaccat gtttcggcat
     1801 ccatccagtc ggtgatttgc tgggcatcct gaccggtggc ttcgacatag gtagcgacca
     1861 ggatgccgtc gactttttcg agcagggcgg cggtgtcctt gaggtctctg gcatcgccat
     1921 aggagatagt ttgagagttg tgaatcatga tcattccgcc aggtgatatg acgacttcgt
     1981 cggctgccag cgcaacccat gaggcggcgc tggcagcgtg gccgtcaatg tgggcgatgg
     2041 tgtgcgcagc gtgctcacgg atgatctgtg ccatggcctg agcggcaaat atttctccac
     2101 ccggagaatc gatgcgcaga tggatggttt tggcatcgat ggccgtcatc tgtttggcaa
     2161 aatcaatggc ggaaatgccc tcaaaaaaac tgtccgacac gatcatgtca tatagccata
     2221 cggtggcttc gtcctgcgag acgttatcga cccggaacag gccttttccg cgattgtcgg
     2281 cgagcagttt gttgagtggg cttttgattt tcatgcgttg tcctgtgcgt tgtcctgttc
     2341 gttgtccttt ttgccggctg atgattctcc attggcgcgg gtgtctccgt ccggcagggg
     2401 ggggaggttt tcgaggcggc ggacttcgtt tggggtcatc catcccggtt cgccggcccg
     2461 acccagggcg atgcgatacc cctcatttct cgccttatag tcgccacgtt cgaggccggc
     2521 ggtggtgaat tctgcgaaat ggattccgtc gcgcaggagc tnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn ncgttcgagg ccggcggtgg tgaattctgc gaaatggatt
     2701 ccgtcgcgca ggagctttcg attgatctcc tgctcgatct tgaccaggtg acgactgagg
     2761 gtgtatttga caaatccgat gccttgctgt tcgatgccgc tgccccagct cgtggccttg
     2821 tcggtgaggc cgatcatgtg gggcgggact ccgtagaagc gggcgatgtc agcggcctga
     2881 taattgcgag tctcgatgag ctgagcatcc tctggattga gtgacaacgc tttgacttct
     2941 ccgccgccgg ttagaatggc cggaatgtgg gagtttccaa ggccggcgta cctggccatc
     3001 caggattccc ggaacagtgc ctgcgcggca gcgtccattc tgccaggcgt ggtgatcacg
     3061 aaatccggcc gggcgccatt actgaaaaag cgcgcggaat actcgtcagc agccagtgat
     3121 aatcccatgc tgttgcgagc ggcgtgccgc agtggagatg cgccgcggca gccgtcgaat
     3181 ccgagcccag gaacatgcag catgtcgtcc tgatcgagcg tgatcatgcg tccgtcgacg
     3241 gctgaatcga tcagttgata ggcaagacgg tcgccattta tcgagactga aacgtctaaa
     3301 gggtgtaggg gctcaagccg gactacatct ggcagcaggc tccgacgacc tcccttgcgg
     3361 tgaatcctgg cgaatccgtc accgtgcagc agcaaggccc agatcaggta ttctcgccag
     3421 acggcggcgc tgcaggcggg ggtcggctcg acgttgagga ggtcggacaa cgggtggtcg
     3481 acgcgctggc gagaatctcc tcctcgccgg taaatgggca gcggcaggct ggagatggct
     3541 ccgccgatca gaccgataca tgcgtaaacc gcgcagatgg ccagcgcggt cctttcattg
     3601 acggcaggtc cggctgtgga cagggacccg gagccggtca gccattcgta aagctgacta
     3661 tctcttccgc cagagctggt ggtgtaggtg gtggcgttgc tgagcttccg agcggcttcc
     3721 cgctgggcct tccaggcggt gaggatcacc gatccgggga tggcaacgcg ctcagcgttg
     3781 taccaggtgg gggcggcgct catagtacga caagctcaag gcaaggagat ttttcttcgc
     3841 tgggcaccat ggctctggac attgcgacga ttgtggcgac ggcggcatcg atcttgttct
     3901 gtgagcgcag tttgcggggg aagatgttct cgttccgatc gctccagact tctacgtttg
     3961 agagcatcca gacgtaggcg ggatttccgt cgtgatgaaa tcgtccggca tcgatgagcg
     4021 cggcgatttt tttcattggt tccgagagat ggcgcgtctg catgggaatg tcgacgactt
     4081 caaatccctc ctcttgaaga ttcggggcga tttcgcggga accccatgcg tcaagcgaaa
     4141 tttcgcggat caagacggtt tctgagctgt cgattacatc atcctgaatt tgtctcaatg
     4201 agatcatgtt gccaggcgtc ggtgtgatat ggcctccgga aacccatgcc tggtagtggg
     4261 agttctccgg cttgtcgacg gctgtctgag gcaggtagtt gcgggagatg gcggtgtagt
     4321 gttcttctcc gtctctttct tccgaaaata gccagatcgc cgaagcgatg tcgactttgc
     4381 tggccaggtc gagaccgatg acgcatggct taccgcgcat cgattcgagc gtaatggcgg
     4441 ggtctccagc ctgctgcagg gcgtgcaggt tgagccacgg agatgcggcg gcgacccatg
     4501 tgttgaggtg ttttgtcttg aatgtgttct gcttgcgcgg gtcggagatg gcgtcgcgct
     4561 gctgcatctt gaggaagtct ccgtcgatcg atacgccata gtttgggttt gccttgcgca
     4621 gggaatcttc ggacgtccaa tcgtccccgt catcaacgcc aaagatgatg ccgaaacgct
     4681 ggtcattttc gacgatcgat tcgaggattt tctgtagctc gacctggtgc aggtagcacg
     4741 gacacgagag gtcagatcca gccgttgtga tgacgacgat tagcggttgc gagcgggctc
     4801 ccatgccggt ctgcatggtg tcgtagagcg ctgaggtttt gtgctcgtgg aattcgtcga
     4861 tgagcgcgca tgatggacta gcgccgtcgc caggattgcc gatcacgggc tcgaacttac
     4921 tgtttgtgga tacgaccgag atattgctgg cattgacggt gactccgtaa gcctgcaggt
     4981 agcgtggcgt ggcgcgcgcc atcagcatgg ctggccggaa tacttccatg gcttgatctt
     5041 gtgtcgtggc tcctgagtag atttcggagc caaattcgcc gtctaccgcc agcatgtaga
     5101 ggccaatcac ggctgccagc gtgctcttgg cgttttttcg aggaatgaac aggtcggcga
     5161 cgcggaatcg gcgctttcct gtctcgcaat gcacccagcc gaagatgctg cagaggatga
     5221 agatttgcca ggtctccagc ttgatgagct ggcggcgagc agcccagtcc cccttgatgt
     5281 gaggcatgag ttcggcgaaa cggcagatgc gctcggcagg gtggtacgcc ttgcccgccg
     5341 aatcggtctg ctgagggttg aaacgatagg gccaggcggg gtcaagcgcg gcgcgcgata
     5401 ggtcgtccag gtgacgctga caggcgagac gatgccattt gcaagcaggg atacgaccct
     5461 cg
//
LOCUS       DAOQEW010000232         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_5923_length_5452_cov_5.429313, whole genome shotgun sequence.
ACCESSION   DAOQEW010000232 DAOQEW010000000
VERSION     DAOQEW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5923_length_5452_cov_5.429313"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLS35_17325"
     CDS             complement(<1..278)
                     /locus_tag="PLS35_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI20487.1"
                     /translation="MTSITEPLHDHHKFCDELFADAEDSAHGGEWVKCQEAFDRFRNE
                     LEAHFSTEENDLFPAFERTTGMAGGPTHVMRIEHAQMRDLIGQMSSAL"
     gene            complement(377..1117)
                     /locus_tag="PLS35_17330"
     CDS             complement(377..1117)
                     /locus_tag="PLS35_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI20488.1"
                     /translation="MKTAQVIYENGEHKWLAIVRDPARPAYLIDTNEYLVSVGDDAIL
                     LDPGGAEIFPATFAAVSTAFDPRKIGSLFASHQDPDIISSLSMWMEFNPQLRCYVSWL
                     WEAFIPHFGGVKESFVAIPDQGMDILVGGNRMQAIPAHYLHSSGNFHLYDPKAKILFS
                     GDVGAALLPQGEDGLFVEDFARHIRHAEGFHRRWMGSDRAKRDWCARVSRMEIDMLCP
                     QHGAIYRGEDVRRFIDWFADLEVGVVRT"
     gene            complement(1140..1745)
                     /locus_tag="PLS35_17335"
     CDS             complement(1140..1745)
                     /locus_tag="PLS35_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20489.1"
                     /translation="MGTAAEGGREAGRELWRIVHINEEIKSIVATAFKINLMAMNAIF
                     LAKRAGQSALGFGVLSNELRRFAQDLQRQMETLRDTTFGSTGTVTALLKQSRLMGVLE
                     RTRNECGPAGRGIVDEHIRHRHSADEQERRMQIAALERRLSQMIADTTQIVELGSVLA
                     RSAKIEAAYGGAFGQSLMQVSSDFEETIAEIQRSLVNLSKH"
     gene            complement(1975..3615)
                     /locus_tag="PLS35_17340"
     CDS             complement(1975..3615)
                     /locus_tag="PLS35_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNI20490.1"
                     /translation="MGMATPLLHAQLAWLSHPQELAEELSKVSSRLWALQWHSWARAL
                     GLPSPDMEKPHPDDTRFSDPVWTESATWDITKEWYLLFTHRIQDMLYNAPGVSSKDRR
                     RAAFWWRKWLNAMAPTNFLLTNPVALAKAAETNGESLMRGMRIFLEDLKAGNIRMTNP
                     SDFTVGKNLATTPGKVVFRNRMLEVIRYTPTTEQVHEVPLVIVTPWINKFYILDINAK
                     KSMVRFLLDQGFQVFITSWKNPKEDMRDVGFDAYLTEGISAIVDCAREVTGSPKVNTI
                     GYCIGGAALATWLAWANRKYAPEEMPVASTTFFTTLVDYHKPGDVEVFLDEGSFRWLS
                     RDMKQKGYLDGKSMAGAFRLLRSNSLIWQYVVHGYLYGETPPPFDILYWNMDTTRMPA
                     AMHEWYLREFYLNNNLIKKDALTVAGQPIDLGRVVQPVYSVAAEDDHIAPWRQTFRLC
                     NYVAGPKRYVLSSSGHILGIVNPPVHPPKREYRVADAERHDTWETWQDKSERRAGSWW
                     DDWMQWLKPKSGGMVSPRPPDGRYAPLCDAPGTYVVEA"
     gene            3885..4328
                     /locus_tag="PLS35_17345"
     CDS             3885..4328
                     /locus_tag="PLS35_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20491.1"
                     /translation="MFKNILVPTDGSQLSADTAKRATSFAKEAGARVVFFFAKPEYPV
                     AFYGEGALIDPTTPEQFAEMAEKQAREILDACETMARESGVDASSVCVTSDVPYEAII
                     EAANTAACDLIFMASHGRKGISGLLLGSETQKVLTHSRIPVLVYR"
     gene            4454..5068
                     /locus_tag="PLS35_17350"
     CDS             4454..5068
                     /locus_tag="PLS35_17350"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNI20492.1"
                     /translation="MLNVALATIQDEHRSLAAVVHGLKFVVREIREGKVHPDFRLLWA
                     MLYYIDAFPEKLHHPKEDSYLFAKLRRRTSEGDQILAELEKQHREGAAYVREMELTLG
                     HFEAGKPGGFESFAGAVETFAETIWNHMNIEERTVIPLAKRVLTDEDWVEIGEAFGAN
                     GDPRFGLNPDHEFRDLFSRIVNLAPSPIGLGPADTGKAIPQGDA"
     gene            complement(5075..5341)
                     /locus_tag="PLS35_17355"
     CDS             complement(5075..5341)
                     /locus_tag="PLS35_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20493.1"
                     /translation="MKRFALILWPSFLVAGVAEMLFFTLVAPQELYLFGEPVRYSMLA
                     TYSIGFFGFWAVCAASSLMTCFLQRTASEVNRRAPDGGNEGRCG"
BASE COUNT         1067 a         1672 c         1620 g         1093 t
ORIGIN      
        1 tggagcgccg acgacatctg gccgatcagg tctcgcatct gggcatgctc gatccgcatc
       61 acgtgggtcg gtccacccgc catgcccgtg gtcctttcga aggccggaaa aaggtcgttc
      121 tcctcggtcg agaaatgcgc ttccagctcg ttacgaaaac ggtcgaacgc ctcttggcat
      181 ttcacccatt cgcctccatg ggctgaatcc tcggcatcgg caaacagttc gtcgcaaaat
      241 ttgtggtgat cgtgcagcgg ttcggtgatg gaagtcatgt cggatgggcg ggggtcaggg
      301 ccgagggttg aggacgaaat cggctgctag tgtccctgct gccccgacac cggacattga
      361 cccgggtcaa gaagtctcac gtgcggacca cgccgacttc caagtcggca aaccagtcga
      421 tgaaccggcg gacgtcctct ccacggtaaa tagcgccgtg ctgcgggcac aacatgtcga
      481 tctccattcg cgacacccgc gcgcaccaat cgcgcttggc gcggtccgat cccatccagc
      541 ggcgatggaa gccttccgcg tgacggatat gcctcgcgaa atcctccacg aacaatccgt
      601 cttctccttg cggcaggagc gctgccccta cgtcaccgga gaacaggatc ttcgccttgg
      661 gatcgtacag gtggaaattc cccgatgaat gcaggtagtg ggcgggaatg gcttgcatcc
      721 ggttcccacc gacgagaata tccatgcctt ggtccggaat ggcgacgaaa ctctccttca
      781 caccgccaaa gtgcggaatg aaggcttccc aaagccaact cacgtaacac cggagttgtg
      841 gattgaattc catccacatc gacagcgatg aaatgatgtc gggatcctga tgagaggcga
      901 acaggctgcc gatcttgcgc ggatcgaatg cggtgctgac tgcggcaaag gtggcgggga
      961 agatttcggc gccgcccgga tccagcaaga tggcatcgtc gccgaccgac actaggtatt
     1021 cattggtgtc gatcaggtaa gccgggcgcg ccggatcgcg aacgatggcc agccacttgt
     1081 gctcgccgtt ctcgtagatg acctgggcag ttttcatgac cgtcttctcc ccctcgtttt
     1141 caatgcttgg acagattcac cagcgagcgc tggatctcgg cgatcgtttc ctcaaagtcg
     1201 ctggagacct gcatcagaga ctggccgaac gcccccccgt aggcggcttc gatcttggcc
     1261 gaccgtgcca gcacgcttcc cagttcgaca atctgcgtcg tgtcggcaat catctgggag
     1321 agacgacgtt ccagtgcggc aatctgcatc cgccgctcct gctcgtccgc cgaatggcgg
     1381 tgccggatgt gctcgtcgac gattccgcgt ccggccggtc cgcactcgtt gcgggtgcgc
     1441 tcaagcacgc ccatcaggcg cgactgtttc aacagcgccg tgaccgtccc ggtgctgccg
     1501 aacgtggtat cgcgcagggt ttccatttgt cgttggagat cctgcgcgaa tcggcgcaat
     1561 tcgtttgaca ggacaccaaa acccaaggcg gactggccag cccttttggc caggaagatc
     1621 gcattcattg ccatgaggtt gatcttgaac gccgtggcga cgatgctctt gatctcctca
     1681 ttgatatgta cgatgcgcca caactcgcgc cccgcctccc gcccgccttc cgctgccgtg
     1741 cccatcgaga tccacccttg gctatggagc cacgctcctg tttacctgcg ccacgcggga
     1801 tcgcctcccg tcccttcccg cacccccggc attaacggaa tcgccggccg gaatgatctt
     1861 cgcccgcacc gaacatcgac ggcggaacgc cggcctgtcg ccgattataa acagagcccg
     1921 agccgagtcc tcgcggccca tacgtgcgcg aaatcgtggg tacacagcat cgtttcaggc
     1981 ttccacgaca taggttcccg gggcatcgca cagcggtgca tatcgcccgt cggggggacg
     2041 cggtgacacc attccgccgg acttgggttt cagccactgc atccagtcat cccaccaact
     2101 gccggcccgg cgttccgact tgtcttgcca ggtttcccaa gtgtcgtggc gctcggcgtc
     2161 ggcgacgcga tactcccttt tcggaggatg cacgggtggg ttcacgatgc cgaggatgtg
     2221 gccggaactg gacaaaacgt agcgcttggg cccggcgaca tagttgcaga ggcggaaggt
     2281 ctgccgccac ggtgcgatat ggtcgtcttc cgcggcaacc gaatagacag gctgcacgac
     2341 ccgaccaaga tcgatcggct ggccggccac cgtcagggca tctttcttga tcaggttgtt
     2401 gttgagatag aactcgcgga ggtaccactc gtgcatcgca gcaggcatcc gcgtcgtgtc
     2461 catattccag taaagaatgt cgaacggagg gggcgtttca ccgtacagat aaccgtgcac
     2521 cacgtattgc cagatcaggc tgttcgaccg caacagccgg aaagcgcctg ccatcgactt
     2581 tccgtccagg tatcctttct gcttcatgtc gcgggatagc cagcggaagc ttccctcgtc
     2641 gaggaacacc tccacatccc cgggcttgtg gtagtcgacc agcgtcgtga agaaggtggt
     2701 gctggcgacc ggcatttcct ccggcgcgta tttccgattt gcccacgcaa gccaagtcgc
     2761 caaggcggct ccgccgatac agtatccgat ggtgttgacc ttcggcgagc cggtcacctc
     2821 gcgtgcacaa tcgacaatgg ccgaaatccc ctccgtcagg tatgcgtcga aaccgacgtc
     2881 gcgcatgtcc tccttgggat tcttccaact ggtgatgaat acctggaaac cctggtcgag
     2941 cagaaaacgc accatgctct tcttcgcatt gatgtccagg atgtagaact tgttgatcca
     3001 cggcgtcacg atgacgagcg ggacttcatg cacctgctcc gtcgtcgggg tgtatcggat
     3061 gacttccagc atgcggttgc ggaacacgac tttgccgggc gtagtggcca aattcttacc
     3121 gaccgtgaaa tcggacgggt tggtcatccg gatgtttccc gccttgagat cctccaggaa
     3181 gatccgcatc cctcgcatca gggactcgcc gttggtttcg gccgcctttg ccagcgccac
     3241 cggattcgtc agaaggaagt tcgtcggcgc catcgcgttg agccatttgc gccaccagaa
     3301 cgcagcgcgg cgacgatcct tgctggagac gccaggtgcg ttgtacaaca tgtcctgaat
     3361 ccggtgggta aacagcaggt accactcctt cgtaatgtcc caggtcgccg attcagtcca
     3421 caccggatcc gagaaacgcg tatcgtcggg gtgtggcttt tccatgtccg ggctcggcag
     3481 cccgagtgcc cgagcccatg aatgccactg cagagcccat agacgtgacg acaccttgga
     3541 cagttcttcc gcgagttcct gggggtggct caaccaggcg agttgagcat gcagaagcgg
     3601 ggtcgccatg cccatggggt ccaggttcga ctcgacgacc tcattgacgg cgcgccacgt
     3661 ctcatggggc gtcatgttcg ctttcggcgc ccaggaatgt ttgttgttcg gaccgttcat
     3721 ggtggtgctc ctagctcgga atggtccgcc gcaacgctgg cagacacagt ggtttatgat
     3781 tgagcaggga acagagggct gtgttgacgc tgatcaaccg cctgtccgac cggatttccg
     3841 cgaaaattca atcggtgggt cactcgccca agcgtggagg tagcatgttc aagaacattc
     3901 tcgtaccaac cgacgggtcg caattgtccg ccgacaccgc gaaacgggcc acttccttcg
     3961 ccaaggaggc gggtgcccgg gtggtcttct tttttgccaa gcccgagtat ccggtggcgt
     4021 tctacggcga gggcgccttg atcgatccga cgaccccaga gcagtttgcg gaaatggccg
     4081 aaaaacaggc tcgcgaaata ctcgatgcgt gcgaaaccat ggcacgggag tcgggcgtgg
     4141 atgcttcgag cgtctgcgtc accagtgacg tgccgtacga agcgatcatc gaagccgcca
     4201 acacggccgc ctgtgatctg atcttcatgg cctcccatgg caggaaaggc atcagcggat
     4261 tgttgcttgg gtcggaaacg caaaaggtcc tgacccactc gaggattccc gttctcgttt
     4321 atcgctgaag tgggccatcc gtttctcgcc ctcgccggat gcggtcccgc cctttcggga
     4381 gtcctgcaat tccgagaggc gttgccggag ccgggcggtg taggcctcac tgggtcgtcc
     4441 tgaaggtcac gatatgctga acgtagcttt ggcaacgatt caagacgagc accggtcgct
     4501 ggccgcggta gtccacggcc tgaaattcgt cgtccgcgag atccgcgagg ggaaggtgca
     4561 tcccgatttc cgcctgctct gggcgatgct gtattacatc gatgcattcc cggagaaact
     4621 gcatcatccg aaggaggaca gctatctgtt tgcgaagttg cgccgtcgga ccagcgaggg
     4681 tgaccagatc ttggccgaac tggaaaagca gcaccgcgag ggagcggcct acgtacggga
     4741 aatggaacta acgctcggtc acttcgaagc cggcaaaccc gggggattcg aatccttcgc
     4801 gggggctgtc gaaacctttg cggaaacgat ctggaaccac atgaacatcg aagagcgcac
     4861 cgtgatcccg ctggccaaac gggtcctgac cgacgaggac tgggtggaga tcggtgaggc
     4921 cttcggcgcg aacggggatc cccgcttcgg cctcaacccg gatcacgaat tccgcgacct
     4981 tttttccagg atcgttaacc tggctccctc accgatcggg ctcgggccgg ccgacacggg
     5041 aaaggcgatc ccgcaggggg acgcctgaac ggctttaccc gcaacgcccc tcgttcccgc
     5101 catcaggggc acggcgattg acctcggaag ccgtccgttg caggaagcag gtcatcagac
     5161 tcgatgcggc gcagaccgcc cagaatccaa aaaacccgat ggaataggtt gcaagcatcg
     5221 aataacggac cggctcgccg aataggtaga gttcctgcgg cgcaacgagc gtgaagaaca
     5281 gcatttccgc aacgccggcc accaggaagg aaggccacag gatcaaggca aaccgcttca
     5341 tcggagtcct cccgctgttg attgaggcgg gcgcccggtt acccagacgc cccgccgctc
     5401 atttcgcgtt gcgcgcgtct tcctccgcgt gcttcttgat gaagttcacc ag
//
LOCUS       DAOQEW010000233         5349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6104_length_5349_cov_9.777295, whole genome shotgun sequence.
ACCESSION   DAOQEW010000233 DAOQEW010000000
VERSION     DAOQEW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6104_length_5349_cov_9.777295"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="PLS35_17360"
     CDS             <1..2429
                     /locus_tag="PLS35_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNI20494.1"
                     /translation="KLRNAHRASNRTGQALKRALQGRKKLLLTATPLQNSLMELYGLS
                     TLIDEHMFGDEAAFRKQFMNSGTGLDELRERLASFAKRTLRRDVLEYIKYTERKALTQ
                     PFNPTDDEQALYERISAFLQKEDSYALPKQQRHLTALILRKLLASSSHAVAATLVTIR
                     ERLQGLLAAGKTEDDGSQLVEQLIAEDDLEQDYLEEEASEAEENAEAPTPAPAEEGKP
                     GAAKDAHAVRAAITTEIAELTAFVDAAQALKTDTKAQALLKALNLGFGKMAELRAPRK
                     AIIFTESKRTQEYLHRFLSANGHAGKLVLFSGTNTHEESTAIYQRWLEEYKGTDRVTG
                     SPQVDRRTALIDHFRKDDGTGAEIMIATEAAAEGVNLQFCALIINYDLPWNPQRVEQR
                     IGRCHRYGQRFDVVVINFLNTRNQADQRVLELLTEKFNLFSGVFGASDEVLGRIEGGL
                     DFEKRILQIYDTCRQPEQIEAAFNALQIELEEVIADRINDTQSQLLENFDEDVHDRLK
                     LRLQDAEARLDKLGRWFWGVTRYALNDRARFDEQSYAFSLSSPPTGIASGRYQLIRGA
                     AQPDMLAHAYRLSHPLGEWSIDASLNAATPVATLKLDYSKHGARVSVIERLRGMSGWL
                     TLARLEVTAFETTEALLFSGLTDDGQVLDQEACEKLMAIPAADKPTLLSTAVPEALLA
                     NSQRAVAATIAQVLEANQRLFNEERDKLERWADDKLLAAEEALKNTKARIAQLKRDAR
                     KAATLQEQDGIQRELSDLERKQRRLRQEIFAVEDEIIAKRDELIASLQQRLQEKTSNE
                     TLFSVRWQVT"
     assembly_gap    2557..2656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2657..>5349
                     /locus_tag="PLS35_17365"
     CDS             <2657..>5349
                     /locus_tag="PLS35_17365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNI20495.1"
                     /translation="YYEQGDFISQRRYKGDTYAIPYAGEEVMLHWANKDQYYTKSGEN
                     FSNYSFKLEDGRTVHLRLAAADTAKDNRKDNDKERRFALLVAKTVTRLDENGDDYEEE
                     LVPVEEVAGSDGKQELIIRFEYAAQPKGTKQEALVNKAVEAVLADASVKARWLALGSR
                     APTEKNPQRTLLEKHLSDYTTKNTADYFIHKDLGGFLRRELDFYIKNEVMHLDDVQNA
                     GAFADIEKNLRMIQCLRSIALELITFLAQLEDFQKKLWLKKKFVVSSHYCITLDRVPE
                     ALWPEVVANRRQWEQWKQLGVWDGDAQGTEEDLKAAPYRMVDTSLFSVDFKSSLLQQV
                     EDMDSAISGLLIHGDNFQALNLLRERYRGEIKCTYIDPPYNTGRDGFMYKDGYQRSSW
                     GCLIREGLAALHPLVTDDGMVFVSNDDNENENLKKIFSDLSYHVKANLVWNTDGHTDN
                     QFDVKINHEYVTLSQKSKFAAYGDVVDPNTRAESNLWKNVAENSITKNGPANPYSEVI
                     LPKGFPVTSEHGVINANVPEPQFFEELGEQKYFPRDLIRKHGVSFPIKRNRVTYSDFK
                     LDNDLTVCAGWANLNKLQRFIQNGCQPFPDEGGVMEFYLTENGTVYYKKTKASARNIV
                     SVLRNFSTTEKMKYALEGMGLAFSYPKPKDLISYLLKVGASPIYLDYFAGSGTTAHAV
                     IDLNREDNGKRNYILIEQGQHFETVIKPRLQKVVFSAEWTSEKPTAPGTGISHCFKVI
                     KLESYEDTLNNLQLRRTSAQGDLLNTLPQQAKDDYLLNYLLDVESRGSLLSVEDFKKP
                     FDYTLNIAVDSAGAFEPRKIDLVETFNFLIGLRVKHIDAQPQRGFVTVTGTLPSGESC
                     LVLWRDCDVLDYEGVSKLCDKLAINPADNEFDVVYINGDHN"
BASE COUNT         1302 a         1548 c         1453 g          946 t
ORIGIN      
        1 acaagctgcg caatgcgcac cgcgccagca accgcaccgg gcaggcgctc aagcgtgcgc
       61 tgcaaggccg caagaagctt ctgctcaccg ccacgccgtt gcagaactcg ctgatggagc
      121 tgtacggcct gtcgacgctg atcgacgaac acatgttcgg cgacgaagct gccttccgca
      181 agcaattcat gaacagcgga acggggcttg acgaactgcg tgagcgcctt gccagcttcg
      241 ccaaacggac actgcgccgt gacgtactgg agtacatcaa gtacaccgag cgcaaggccc
      301 tcacccagcc gttcaacccg actgacgacg agcaggcgct gtacgaacgc atctcggcct
      361 ttctgcaaaa ggaagactcc tacgccctgc ccaagcagca gcgccacctc acggcactga
      421 tcctgcgcaa gctgctggcc tccagttcgc acgccgtcgc tgccaccttg gtcaccatcc
      481 gcgagcgcct gcaaggcctg ctcgcagcag gcaaaacgga agacgacggc agccaactgg
      541 tcgaacagct cattgccgag gacgacctgg agcaggacta cctggaagag gaagccagcg
      601 aggcagaaga aaacgccgaa gccccgacgc ctgcgccagc cgaagaaggt aaaccgggcg
      661 ctgcaaaaga tgcccatgcc gtccgcgccg ccatcaccac agagatcgcg gagctgaccg
      721 cattcgtcga tgccgcccaa gccctgaaaa ccgacaccaa ggcacaggcg ttactcaagg
      781 cgctcaacct gggcttcggc aagatggccg agctacgtgc gccacgcaag gccatcatct
      841 tcacagaatc caagcgcacg caggagtacc tgcaccgctt cctctcagcc aacggccacg
      901 cgggcaagtt ggtgttgttc agtggcacca acacccacga ggaatccacc gccatctacc
      961 agcgctggct ggaagagtac aaaggcacgg atcgcgtcac cggctcgccg caggtagatc
     1021 gccgcaccgc gctgatcgac cacttccgca aggacgacgg caccggcgcg gaaatcatga
     1081 tcgccaccga agcggctgcc gaaggcgtca acctgcagtt ctgcgcgctg atcatcaact
     1141 acgacctgcc gtggaacccg cagcgcgtcg agcaacgcat tggccgctgc catcgctacg
     1201 gtcagcgttt cgatgtggtg gtcatcaact tcctcaacac ccgcaaccag gccgaccagc
     1261 gcgtgctgga actgctcacc gagaaattca acctgttctc gggcgtgttt ggcgcaagcg
     1321 atgaagtgct aggccgcatc gagggtggcc tcgattttga gaaacgcatc ctccagatat
     1381 acgacacctg tcggcaaccc gagcagatcg aagcagcctt caatgccctc caaatagagc
     1441 tggaagaagt cattgccgac cggatcaatg acacccagtc acagctgctg gaaaattttg
     1501 acgaggatgt ccacgaccgg ctcaagctgc gtcttcagga cgccgaagca cgtctcgaca
     1561 agctggggcg ctggttctgg ggcgtcactc gctatgcgct caatgaccgc gcacgcttcg
     1621 acgagcagtc ctacgcgttc tcattgagct cgccgccaac tggtatagcc agtggccgct
     1681 accagctcat tcgtggtgcg gcgcagccgg acatgctggc gcacgcctac cgcctcagcc
     1741 acccgctggg tgagtggagc attgatgcca gcctgaatgc ggcaacgccc gtcgccacct
     1801 tgaagcttga ctacagcaaa cacggggcac gggtatccgt catcgagaga ctgcgaggta
     1861 tgtccggctg gctgacgctg gcccggctgg aagttaccgc tttcgagacc accgaggcac
     1921 tgctgttctc aggcctcact gacgatggtc aggtgttgga tcaggaagcc tgcgaaaagc
     1981 tgatggcgat tccagcagca gacaaaccca ctctattgag caccgccgtg cccgaggcgc
     2041 tgctggccaa cagccagcgc gcggtcgccg cgaccattgc ccaggtgctg gaagccaacc
     2101 agcgcctctt caatgaagaa cgcgacaagc tggaacgctg ggctgacgac aagctattgg
     2161 ccgcagagga agcccttaag aacaccaagg cgcgcatcgc ccaattgaaa cgggacgcgc
     2221 gcaaggctgc cacgctacaa gaacaggatg gcatccagcg cgaactgtct gacttggagc
     2281 gcaagcaacg ccgtctgcgg caggagatct ttgctgtgga ggacgagatc atcgccaagc
     2341 gcgacgaact gattgcctcg ctccagcagc gcctgcaaga aaaaacaagc aacgagaccc
     2401 tgttcagcgt gcgctggcag gtcacctgaa acgcaaatcc ttcgaagaat cagatatccc
     2461 gagatcaggg caacccatga ccaccaaaca gaatcccaaa ttccaggagc tcgtcgccaa
     2521 gctgcgcgag atcttccaga tcgaccgccc ggaactnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnntact acgagcaagg cgacttcatc agccagcgcc gctacaaggg
     2701 cgacacctac gccatcccct acgcgggcga agaggtgatg ctgcactggg ccaacaagga
     2761 tcagtactac accaagagcg gcgagaactt cagcaactac agcttcaagc tcgaagacgg
     2821 ccgcacggtg catttgcgcc tcgccgccgc cgacactgcc aaggacaacc gcaaggacaa
     2881 cgacaaggag cgtcgctttg ccctcctggt ggcgaaaaca gtcacccgac tagatgaaaa
     2941 cggcgacgac tacgaagagg agctggtgcc agtggaggaa gttgcgggca gtgatggcaa
     3001 gcaggaactg atcatccgct tcgagtacgc cgcccagccc aaaggcacca aacaggaggc
     3061 gctggtcaac aaggccgtag aggccgtgct ggcggacgcg tccgtcaagg cccgctggct
     3121 ggccctgggc agccgcgcgc ccaccgagaa gaacccgcag cgcaccctgc ttgaaaagca
     3181 cttgagtgac tacaccacca agaacacggc tgactacttc atccacaagg atctcggtgg
     3241 tttccttcgt cgcgagttgg acttctacat taagaacgaa gtcatgcatc tggatgatgt
     3301 gcagaacgcg ggcgctttcg cggacatcga aaagaacctg cgcatgatcc agtgcctgcg
     3361 cagcatcgcg ctggagctga tcactttctt ggcccagttg gaagacttcc agaagaagct
     3421 gtggctgaaa aagaagttcg ttgtctccag tcactactgc atcacattgg atcgagtgcc
     3481 ggaagcgctg tggccggaag tggtggcgaa ccggcggcaa tgggaacagt ggaaacaact
     3541 gggcgtttgg gatggcgatg cgcaggggac agaggaggac ttgaaggctg caccgtatcg
     3601 gatggtggat acaagcttgt tttcagtcga tttcaagtcg tcgttactgc agcaagtgga
     3661 agacatggat tcggctatta gcggcctcct cattcacggg gataacttcc aagcgctgaa
     3721 cttgctaaga gaacgctatc ggggagagat caaatgtacg tacatcgacc cgccgtataa
     3781 caccggtcgt gacggcttta tgtacaaaga cggctatcag cggtctagct ggggctgttt
     3841 gattcgcgaa gggctggcag cgctacatcc cctggtgacc gacgacggaa tggtttttgt
     3901 tagcaatgat gacaacgaaa acgagaactt gaaaaaaatc ttttccgacc tgtcgtatca
     3961 cgtgaaagcg aacttggttt ggaatacgga tggccacact gacaaccagt ttgacgtcaa
     4021 gattaatcat gaatatgtaa ctttatcgca gaaatcaaag ttcgccgcat atggtgatgt
     4081 ggttgatcca aatactcggg ccgagagcaa tttgtggaaa aacgttgcgg agaattcaat
     4141 cacaaaaaac gggcctgcaa atccatactc tgaagtcatt ttgccaaagg gcttccccgt
     4201 cacttccgaa catggcgtga ttaatgcgaa tgtgcctgag ccacagtttt tcgaggaatt
     4261 gggagagcag aaatattttc caagggatct aatcaggaag catggtgtct cgttcccaat
     4321 caagcgcaat cgtgtgacct attctgactt caagctagac aatgatctga cagtttgtgc
     4381 gggctgggcc aacctaaaca agcttcagcg gttcatccag aatggatgcc aaccgtttcc
     4441 cgacgagggt ggggtcatgg agttttacct gactgagaac ggaacggttt attacaaaaa
     4501 aaccaaagca tcggcacgaa atatcgtttc cgtattgcgg aatttctcta ccaccgagaa
     4561 gatgaaatac gccctcgaag gtatgggtct agcgttctcc tatccaaaac cgaaagacct
     4621 catttcctac ttattaaaag ttggcgcgtc gcctatctac ttggactact tcgccggttc
     4681 cggaacgacg gcacacgcgg taatcgacct caacagagag gataatggaa aaaggaacta
     4741 catcctgatc gaacagggtc agcactttga gactgtcatt aaacctcgac ttcagaaggt
     4801 cgttttctca gcggagtgga caagcgagaa accaactgct cctggcaccg gcatctccca
     4861 ctgcttcaaa gttatcaagc tcgaaagcta cgaagacacc ctgaacaacc tgcaactgcg
     4921 ccgtacgtcc gcgcagggcg atctgctgaa caccctgcca cagcaggcca aggacgacta
     4981 cctgctcaac tacctgctgg acgtggaaag ccgtggctcg ctgctgtcag tggaagactt
     5041 caaaaagccc ttcgactaca ccctcaatat tgctgtggat tcggcaggcg ccttcgagcc
     5101 gcgcaagatc gatctggttg aaaccttcaa tttcctgatc ggcctgcgtg tgaagcacat
     5161 cgacgcgcag ccgcaacgcg gttttgtcac cgtcaccggc acgctgccca gcggtgaaag
     5221 ctgcctcgtg ctgtggcgcg actgtgatgt gctggattac gaaggcgtca gcaagctctg
     5281 cgacaagctg gccatcaacc cggcggacaa cgagtttgac gtggtctaca tcaacggcga
     5341 ccacaacat
//
LOCUS       DAOQEW010000234         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6147_length_5322_cov_7.203152, whole genome shotgun sequence.
ACCESSION   DAOQEW010000234 DAOQEW010000000
VERSION     DAOQEW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_5322_cov_7.203152"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="PLS35_17370"
     CDS             <1..1108
                     /locus_tag="PLS35_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20496.1"
                     /translation="TAHDEEGHTVPNWVTPQLLQLYLQYVRNHALLDRRLTPDLYTLV
                     LAAKQMAGDDFAIRLLETAKSYAYQEIEGKHLPQLSAGLGQFALPDEQIVRATNRLQG
                     APVTWRSLSLRPRPPRRTSQRWSYLWNPNRQCSWPPEDSKIESFHTHVRDQAKAIIGA
                     DLAKSEKFTTSMKDGIDIRESLRHWHQRAKPGRPNRLELYVKEIPPARGNVDTVIFLF
                     DTPADPDQYSWQATWYAEHAQESTLCFYATPFLNNMIAPGIGQSLYGGALFIFPPRPI
                     PDIWTDEALGFATTLEERLIAAGAAHSHETHVALVTPVPPKARWRQIASRSGRRLVPI
                     PLSRFSGQLIDRLRRFHVLNGQDIRSFAAQFIRE"
     gene            1101..3128
                     /gene="ligA"
                     /locus_tag="PLS35_17375"
     CDS             1101..3128
                     /gene="ligA"
                     /locus_tag="PLS35_17375"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNI20497.1"
                     /translation="MSNPPMADRSIEQRITALRREIRHHDHLYYTKDRPEISDAEYDR
                     LFRELVDLETAHPDLITADSPTQRVGAPPLSELTKVSHEKPMLSLDSIVDQEDVRAFD
                     QRMKRELETDQPVYTAEPKFDGLSVELVYDQGRFVRGATRGDGMIGEDVTVNLRTIRS
                     LPLQLHAETGPPAHLVVRGEVYMRLDEFQSLNRRMTERGEEGFANPRNAAAGSLRQLD
                     SRITASRPLTVTCYDIMAIAGPMPATHWDELEALAAWGLPVPEHRQRCRSIDEVLTFH
                     QTTDAMRDDLPFEIDGVVVKLDRRDWQDQLGSKSRSPRWAIAYKFAPRKEITVIQDIA
                     VSVGRTGTLTPLALLKPVEVGGVTISRATLHNADEVARKDVRVGDTVKVERAGDVIPA
                     IAERVPVPGEERQAPFVMPDHCPVCGSAVAREGAYFYCTGQTVCMAQLKGAIEHFASK
                     SALNIDGLGKKTVAQLVDAGMVKDLADLYSLTKEQLLTLEGFADRSASLLMDSIERSK
                     AVSLERLLMGLGIRQVGQHIARVLAKHFGTLPRVMEATQDELLHVREIGPEISESLAS
                     FFTEARNREVIERLVARGLTVAPPVAEASPGGRTLTGKTFVFTGGLAAYSRDQAKELV
                     ERQGGQVSSSVSKKTAYVVAGIDPGSKLDQAQKLGVKILTEAEFTDLVTPG"
     gene            complement(3103..4416)
                     /locus_tag="PLS35_17380"
     CDS             complement(3103..4416)
                     /locus_tag="PLS35_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNI20498.1"
                     /translation="MDIIVLIFLILLSAVISVVEVGFYSVNDTKLRALADTGSKRAEM
                     ALHLRTDPQRLLLTILVGDRLVDTATASLATIIALNRFGGQGLEGVLGEAFAVLVGIL
                     TFVLLVFADLVPKTLAAKYSVPVVLNMAYPAYAAQQVLTPIMFFVVPLIYKLTGGKGL
                     NVPFVTEEELKIMLDQSSKSGAIEAQEVKMIKNVFQLKDITAEDCMTPRIYMFSLDCN
                     QYLREAKELLFKSKYSRIPLYEGTLDNIIGILYKTKALTALAQGHTEMKLRDIAQPAL
                     FIPHTKSADDLMKQFQLDKRHMAIVVNEFGGVMGLVTLEDLLEEVVGEIVDETDITEE
                     LIKRIGKNQILVHGRTEVRKVNDFLKVDLGDDAVTISGLVQHELGRIPKVGEEVHIAN
                     CRLVIHEADPRVIKSVQIYKEDKHPALPDQPVEEHVPVTQASPDR"
     gene            complement(4681..>5322)
                     /locus_tag="PLS35_17385"
     CDS             complement(4681..>5322)
                     /locus_tag="PLS35_17385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNI20499.1"
                     /translation="TWLHGCSGWSPARPSYPPGYPLGFVERGSASWYGPGFHGNRTAN
                     GEVYDMHKLTAAHRTLPLGSVAVVRSLTTGRQVTVRINDRGPFARGRILDLSLAGAQA
                     VGMVGRGTDEIELRVISYQGRAGEMGWLRVQVGSFADPSNAQALVQRLRNAYPGSRAV
                     AVDLPDGRRYRVYAGQFRTEADAEQAAAHLKRALDTDPFIVRDDSESPATGNP"
BASE COUNT         1108 a         1630 c         1560 g         1024 t
ORIGIN      
        1 aaccgcgcat gatgaggagg gacacaccgt ccccaattgg gtcacgccgc agctgctgca
       61 gctctatcta caatacgtgc ggaaccatgc actgctcgac cgccgcctga cgccggactt
      121 gtacacgttg gtgctcgccg ccaaacagat ggccggcgac gactttgcga ttcgcctcct
      181 cgaaacggcc aagagttacg cttatcagga gatcgaaggc aaacatctgc cgcagctttc
      241 cgccggactg ggccaattcg ccctgccgga tgagcagatc gttcgggcga cgaaccggct
      301 ccagggcgca ccggtgacct ggcgctcgct gtcgctccgc ccgcgtccgc cgcgcaggac
      361 cagccagcgg tggtcctatc tctggaatcc gaatcggcaa tgttcctggc cgccggaaga
      421 ctccaagatc gaaagttttc acacgcacgt ccgcgatcaa gccaaagcga tcatcggcgc
      481 agacctggcc aagagcgaaa agtttacgac ctcgatgaaa gacggcatcg acattcggga
      541 aagtttgcgc cactggcacc aacgcgccaa gccagggcgc ccgaaccggc tggaactcta
      601 cgtcaaagaa atccctccgg cacgcgggaa tgtcgatacc gtgattttcc tcttcgatac
      661 gccggccgat cccgatcagt acagctggca ggccacctgg tatgccgaac atgcgcagga
      721 atccacactc tgtttttatg ccacgccgtt cttgaacaac atgattgccc ccggcatcgg
      781 gcaatcactc tacggcggcg ctctgttcat ctttccacct cgcccgatac ctgacatctg
      841 gacggatgag gcgctggggt ttgcgacaac gctcgaggaa cgtttgatcg ccgccggagc
      901 tgcccattca catgagacac acgtggccct ggtgacgccg gttcctccca aggcgcggtg
      961 gcggcagatc gcctcacggt ctggacggcg cctggtgccg attccgttga gtcgcttttc
     1021 gggacagttg attgaccggt tacggcgatt ccatgtgctg aacggacaag atatccgcag
     1081 cttcgccgcg cagttcattc gtgagtaatc cacccatggc cgaccgcagc attgaacaac
     1141 ggatcacggc gctccgccgc gagatccggc atcacgacca tctctattac acgaaagatc
     1201 gtcccgagat ttccgatgcc gaatatgacc gcctctttcg tgaactcgtc gatctggaaa
     1261 ccgcgcatcc tgatctgatc accgcagact cgcccaccca acgggtcggg gcgccgcctt
     1321 tgagtgagct caccaaggtg tcgcacgaga aaccgatgct gagcctggat tcgatcgtcg
     1381 atcaggagga tgtccgcgcc ttcgaccagc gcatgaaacg cgagctggaa accgaccagc
     1441 cggtctacac agccgaaccg aagttcgacg gcctgtcagt ggaactggtc tacgatcagg
     1501 gccgcttcgt gcgcggcgcg acgcgtgggg acggcatgat cggtgaagat gtcacggtca
     1561 atctccgcac gatccgctcg ctgcccttgc aacttcatgc cgaaacgggt ccaccggcgc
     1621 atctggtggt ccgcggcgaa gtctacatgc ggctggatga gtttcagtct ctcaatcggc
     1681 ggatgacgga gcgcggcgaa gaaggctttg ccaatccgcg caatgcggcg gcgggatcat
     1741 tgcggcagct cgatagccgc atcaccgcct cccgtccctt gaccgtcacc tgttacgaca
     1801 tcatggccat cgccggcccg atgcccgcca cacattggga cgagttggaa gcgctcgccg
     1861 cctggggctt gccggttccg gaacatcggc agcgttgccg gtccatcgac gaggtcctca
     1921 cgttccacca gacgaccgac gcgatgcggg acgaccttcc gtttgagatc gacggcgtcg
     1981 tggtcaaact ggatcgccgt gattggcagg atcaactcgg cagcaaatcg cgcagtcccc
     2041 gctgggccat tgcctacaag tttgccccga ggaaggaaat cacggtcatt caggatatcg
     2101 cggtctccgt gggccggacc ggcacgttga cgccgttggc gctgctcaaa cccgtagagg
     2161 tgggcggagt caccatcagc cgcgcgacgc tgcacaatgc cgacgaagtg gcgcgtaaag
     2221 acgtgcgcgt cggcgatacg gtcaaggtgg agcgagccgg cgatgtgatt ccggcaatcg
     2281 ccgagcgggt gcccgtcccg ggtgaggagc ggcaggcgcc gttcgtcatg cccgaccatt
     2341 gtcccgtctg cggttcggcg gtggcgcgcg aaggcgctta cttttactgc acgggacaga
     2401 ccgtctgtat ggcgcaactc aaaggtgcga tcgaacactt cgcctcgaag agcgcgctga
     2461 atatcgacgg gctggggaag aaaacggtgg cccagctggt tgatgcagga atggtcaaag
     2521 acctcgcgga tctgtacagc ctgacaaaag aacaactgtt gaccctggag ggcttcgccg
     2581 accgatcggc cagcctgctc atggactcca tcgagcgaag caaggccgtc tcactggagc
     2641 gtttgttaat gggattaggg attcggcagg tggggcaaca cattgcacgc gtgctggcga
     2701 agcatttcgg cacactcccc cgagtgatgg aagcgacgca ggacgagttg ctgcacgtgc
     2761 gggaaattgg tccggagatc tcagagagcc tggcctcgtt cttcaccgaa gcgcgaaatc
     2821 gggaagtgat cgagcgcctc gtcgcccggg gattgacggt cgctccgccg gtggcggaag
     2881 ccagtccagg cggccggacc ctgaccggca aaaccttcgt gtttaccggt ggcctcgctg
     2941 cctacagtcg cgatcaggcc aaagaactgg tcgaacggca gggaggacaa gtgtcgtcga
     3001 gtgtgagtaa gaaaacggcg tatgtcgtgg cgggaatcga ccccggatcg aaactcgatc
     3061 aggcgcagaa gctcggcgtg aagattctga cggaagcgga gtttaccgat ctggtgacgc
     3121 ctgggtgacc ggcacatgct cctcgaccgg ctggtccggc aacgcggggt gtttgtcttc
     3181 cttgtagatc tggacgcttt taatcacccg cggatccgct tcatggatca cgagccggca
     3241 gttggcgata tgcacttcct cgccgacctt gggaatccgg ccgagttcgt gttgcaccag
     3301 tccgctgatc gtcacggcat cgtcccccaa gtcgaccttc aagaagtcgt tgaccttccg
     3361 cacttcggtg cggccgtgca ccaggatctg gttcttgccg atccgtttga tcaattcttc
     3421 ggtgatgtcc gtctcatcga cgatttcgcc gaccacttcc tcgagcaaat cctccaacgt
     3481 gaccagaccc ataacgccgc cgaattcgtt cacgacgatc gccatgtggc gtttatccaa
     3541 ctggaactgt ttcatcaaat cgtcggccga tttcgtgtgc gggataaaca acgccggctg
     3601 tgcgatatcc cgcaacttca tttcggtatg gccctgggcc agggcggtca acgccttggt
     3661 cttgtagagg atgccgataa tgttatccag cgtgccttcg tacagcggaa tgcgcgagta
     3721 cttcgacttg aacaacagct ccttcgcttc gcgcaggtat tgattgcagt cgagcgagaa
     3781 catgtagatg cgcggggtca tgcaatcttc cgccgtaatg tccttcagct ggaagacgtt
     3841 cttgatcatc ttcacttcct gggcctcgat ggcgccgctc ttgctgctct ggtccagcat
     3901 gatcttgagc tcttcttccg tgacgaaggg cacattgagc cccttcccgc cggtaagctt
     3961 gtagatcaac ggcacgacga agaacatgat cggcgtgagc acctgctgtg ctgcataggc
     4021 gggatatgcc atgttcagca cgacgggaac agaatatttc gccgcaaggg tcttggggac
     4081 gagatcggcg aacaccagca acacaaatgt caggataccg acgagcaccg caaaggcctc
     4141 gccgagtaca ccttcaagcc cctgccctcc gaaccggttc aaggcaatga tcgtggccaa
     4201 cgacgcggtc gccgtatcga ccagccggtc gccgaccaga atggtcaaca ggagccgttg
     4261 cggatcggtg cgcagatgca gggccatctc tgcccgcttg ctgcccgtat cggccaaggc
     4321 gcggagtttc gtgtcgttga cggaatagaa gcccacttcg acgacggaga tgaccgccga
     4381 caacaggata aggaagatca agacgataat gtccataaga cctatcgagg ctaaaggtac
     4441 ggaacaacga tgtcagggta aaaaatggat tcttggatca gttcgaagcc tagggtccga
     4501 actgcaagac ttgcccgatg tcgtgagaag acgacgaaga aaggtcctct cgggtcacca
     4561 tcatccatta cctattacac ttagcatagg ccgagagtcg catcaagcct tgagaggaaa
     4621 tcttgctgcg gacaatgcat cacgatatca accgtttaaa atatttaccc gccacaacac
     4681 tcatggattt cccgtagcgg gcgactcact gtcgtcccgc acaatgaacg gatccgtatc
     4741 cagggctcgt ttcaaatgtg cggcggcctg ttcggcgtcg gcttccgtgc gaaattggcc
     4801 ggcatagaca cgataacgtc gcccgtccgg cagatcgacc gccaccgctc gagaaccggg
     4861 atacgcattc cgtagccgct gcactagagc ctgcgcgttg gacgggtcgg caaaggagcc
     4921 gacctgcact cgcaaccagc ccatctctcc tgccctgccc tgatagctga taacacgcag
     4981 ttcaatctcg tccgtgcccc ggccgaccat acccaccgcc tgggcaccgg ccagcgagag
     5041 gtccagaatc cttccccgcg cgaatggccc gcgatcattg atgcgcacgg ttacctgccg
     5101 tcccgtcgtc aacgagcgca ctaccgccac cgaacccagg ggcaatgtcc ggtgcgccgc
     5161 cgtgagttta tgcatgtcgt agacttcgcc gttggccgtg cgattgccgt gaaagccagg
     5221 cccgtaccag gacgcgcttc cacgctccac gaatcccaag ggataccctg gcggatagga
     5281 cgggcgcgcc ggactccagc cggagcagcc atggagccat gt
//
LOCUS       DAOQEW010000235         5313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6169_length_5313_cov_6.042982, whole genome shotgun sequence.
ACCESSION   DAOQEW010000235 DAOQEW010000000
VERSION     DAOQEW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6169_length_5313_cov_6.042982"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLS35_17390"
     CDS             <1..677
                     /locus_tag="PLS35_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20500.1"
                     /translation="QRTAKAIGAEEEKKARLEDAFTWYVRAKAPADAGRMQRRLVEAK
                     PDDINTLSHAIDYFSQQNDKAQEEAMRAQARKNVEQALAEEEKRFASFSKDSLHELGR
                     ARDWAYYAKTGEDRIRARAAQRGDTLAAEDGRKFLRLAMSYYEQADRPDGVKKIREKA
                     RMLAKRHEAKGEGEIAAEFYAMAGDDKQADTVQKQTEQRQAQAEEGRKKTFKKEQADL
                     EKALGF"
     gene            953..>2727
                     /locus_tag="PLS35_17395"
     CDS             953..>2727
                     /locus_tag="PLS35_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20501.1"
                     /translation="MAKKQSGTVSWATSAEWDVRTLEPARGGRRGATEPPDPFLVNLT
                     ATKRWEVEQTLTATPKARRGETAPAPPLTLDVEADPDDVYIVMTRYASGAIRFHMPNE
                     PARRGRRRSARTLYRFSIPVPAAPLAEPGGRRGFISAAIKAVVLKVAGKLADYALAKL
                     ALLWETATWKYKGRREGWLSVTGEALGGQQMLPAADLSSLSTTGRNLLFLHGTFSSTT
                     AAFNGLATTRGSRGKTFFDELQDLYGNRIYGFDHFTLSRTPEENVRMLLEALPDRTTT
                     FDVITHSRGGLVLRHLVERRDRFGALADRFAVGRAVLVASPNGGTPLASPDHVTQYTN
                     WLSNVLELFPDNPFTTGVEFVSEALSWIARRLVGSLPGLASMDNNGEIIQALQAAPNP
                     PGQAYSALVANFEPDAGLLQRIIDAGADLFFPTANDLVVPTEGGWRVDPGSGSVPAIP
                     GDRIGCFGVGGNLSQQQPVNHVNFFQDPGTVDFLVRALRGESQLLTPIDCNGDLPPGR
                     RRGAALQKTPPAAVPTVRREEQPTTRPTLPAVQVNPPVVAVEPYPEPDDVFHIALIAP
                     KAGADEAQLIATFRNARVMEHLHTGG"
     assembly_gap    2728..2756
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2757..4426
                     /locus_tag="PLS35_17400"
     CDS             <2757..4426
                     /locus_tag="PLS35_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNI20502.1"
                     /translation="ATGRRGTSDHAPTQWDRIKAGQAHIHGYINGDPAYPQLPDETAL
                     QQMGSALFMALFPGQVRRLYDAARSEQVSQRLNLIFTSDIGWIADQSWEFIYDPDRRT
                     FLALEEVNFTRNVLTAIPAERIPARPTMRILVVVAQPLGLGALSVEEEADVIRSGFRR
                     LLDAGLAEVELLLDATPELLHRALESAAAPIDVLHFIGHGEYNEQTDCGYLIFENQDG
                     GEQRLDSSTLRQIVCRRGIRLVCLNACETGRGGRQDFSRGVAQALIAGGVPAVVANQY
                     PVLDVSATAFSRHFYWALAMGHPIGDAAREARVSVNYAISGEAIDWAVPVVFARNPAQ
                     RLCVPRTAAGYERSRRAVVRQRRRAVEDRIKIGIWNAHRMIPHLPDICERLTKAQDRY
                     AFETVSFPAPIGIWRREQEKDQAFVVAETLYDRLKGKPKELGVETLICMINFPMKDAR
                     TRYLYYWRRDPLIVTSTFGLLEQLNEREFTVERMMAHLAAAVVAGIPPHPRRVGPADC
                     PLFYNEQRDIRLIAGRLTFCANCRKQFKDKPAQERLRAAERLLAAYP"
     gene            4652..>5313
                     /locus_tag="PLS35_17405"
     CDS             4652..>5313
                     /locus_tag="PLS35_17405"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20503.1"
                     /translation="MSTKIKTFVLCCSTLVLTACPQPHAVPPPSFPPSWETLIRKGDI
                     EQEYWRYPEAEYYYSQAVTRAQEFGADDARLARSLRSLSSVVSSRGNYAAAKQYGEQA
                     LGIYRHLFGPESGEVGMTEYILGSIAAEHGSFSEAKQLYMQSQRHIAAAYGPEDPRLG
                     QPMIDLAGLYLMTNRMIEAENLLRQVITLSDKPENRDPSRLSLALRHLGALLLTQKRL
                     DEA"
BASE COUNT         1110 a         1669 c         1561 g          944 t
ORIGIN      
        1 agcaacgcac cgccaaggcc attggcgcag aagaggaaaa gaaggcacgc ctggaggacg
       61 cgtttacatg gtacgtgcgc gcgaaggcac ccgccgatgc gggccgcatg caacgcaggc
      121 tcgtcgaggc caaaccggac gacatcaaca cgctgagcca cgccatcgac tacttctccc
      181 agcagaacga caaggcgcag gaagaagcga tgcgcgccca ggctcgcaaa aacgtcgagc
      241 aggccctcgc cgaggaagaa aagcgatttg cctcgttcag caaggattca ttgcatgaac
      301 ttgggcgggc gcgcgactgg gcctattacg ccaaaaccgg agaggaccga attcgcgcgc
      361 gggcggcaca acgcggcgat accctcgcgg ccgaggacgg tcgaaagttc ctccgtctcg
      421 ccatgtccta ttacgagcag gccgaccgtc cggacggcgt caaaaagata cgcgagaagg
      481 cgagaatgct ggcgaagcgg catgaagcca aaggtgaagg cgagatcgcg gcggagtttt
      541 acgcgatggc cggcgacgac aagcaggccg ataccgtcca gaagcaaacc gagcagcgcc
      601 aggcgcaggc cgaagaaggg cgcaagaaga ccttcaaaaa agagcaggca gatctggaga
      661 aggcactcgg cttctgagca gccctcctcg cgagaaacta atgctcgcgt tcttctcgtg
      721 caggccaccg atggcctgcg tgtgtgcttg ctgcctcaac gccccccttg cctgatcgtc
      781 ccggttatgg gataagcgcg gcgaacaatc ctcgtcatgc agagattgta acggatcctt
      841 cgtcggcaca tccggatgac cggcgctcca cgggagacga ccgacgaggc aacccttcgt
      901 caacactacg atcagacgtc atcaggcggc acgagaccag caggagcaca ttatggcgaa
      961 aaaacaatcg ggcacggtga gctgggcgac atcggcagag tgggacgttc gcacactgga
     1021 gcccgctcgt ggcggacggc ggggcgcgac cgaacccccg gacccctttc tggtgaatct
     1081 cacggcaacc aaacgctggg aagtcgagca gactctcaca gcgacgccca aagccagacg
     1141 cggcgagacc gcccctgcgc cgcccctcac cctggatgtc gaagccgacc cggacgacgt
     1201 ctatatcgtc atgacgcgtt atgcgtcagg agccatccgc ttccacatgc cgaacgaacc
     1261 ggcccggcgc ggacgacgga ggagcgcacg aaccctctac cgattttcga ttcccgttcc
     1321 tgccgcacct ctcgccgagc caggcggccg ccggggattc atcagtgccg ccatcaaggc
     1381 cgtggtcttg aaggtcgccg gcaaactcgc cgattatgcg ctcgccaaac ttgccttgtt
     1441 gtgggaaacc gccacctgga aatataaagg acgccgggaa ggatggctgt ccgtcacggg
     1501 cgaagcgctt ggtggccaac aaatgctccc ggccgctgat ttgtcatcgc tcagcaccac
     1561 tggccgcaat ctgctcttcc tgcacggcac cttttccagc acgacggccg ccttcaatgg
     1621 attggccaca acccggggca gccgcggcaa aacattcttc gacgagctgc aggatctcta
     1681 cggcaaccgc atctatggat tcgaccactt caccctcagc cgcacaccgg aagaaaacgt
     1741 gcgcatgttg ctggaagccc tgcccgatcg caccaccacc ttcgatgtga tcacccattc
     1801 gcgcggcgga ttggtcctgc gccacctcgt ggaacgtcgt gaccggttcg gggcgctggc
     1861 ggaccgcttc gccgtcggcc gggccgtgct ggtcgcaagc ccgaatggcg gcactccact
     1921 cgcatcaccg gaccatgtga cgcaatacac caactggctg tccaacgtgc tcgagctgtt
     1981 tcctgacaat cccttcacga ccggtgtgga attcgtgagc gaggcgctgt cgtggatcgc
     2041 ccggcgactg gtcggtagtt tgcctggtct ggcctctatg gataacaacg gcgagatcat
     2101 ccaggcatta caggcggcac cgaacccgcc aggtcaagcc tattccgccc tcgtcgccaa
     2161 tttcgaaccg gatgcgggct tacttcaacg gatcatcgat gcgggcgcgg atctgttttt
     2221 ccccaccgcc aacgatctcg tcgtcccgac tgaaggcgga tggcgagtgg atccaggcag
     2281 cggctccgtc cctgccattc ccggggaccg catcggttgt ttcggagtcg gagggaattt
     2341 gtctcagcag cagccggtga accatgtgaa ctttttccag gatcccggca cggtggattt
     2401 tctggtccgc gcgttgcgtg gtgaatcaca attgcttacg ccgattgact gcaatggcga
     2461 cctgccaccc ggacgacggc gaggcgcagc ccttcagaaa acaccgcccg ccgctgtacc
     2521 gacagtccgc cgcgaagaac agccaacaac gaggccgacg cttcccgcag tgcaggtgaa
     2581 tccgccagtc gtcgcggtcg agccataccc cgaaccagac gacgtgtttc atatcgccct
     2641 gatcgctccc aaagcaggcg cggatgaggc gcagctcatc gccaccttcc ggaatgcccg
     2701 cgtcatggaa cacctgcaca ccggcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc
     2761 cacaggccgc cggggaacca gcgatcatgc gccgacgcag tgggatcgta tcaaggccgg
     2821 acaggcgcac attcacggct atatcaatgg tgatcccgct tacccgcaac tcccggatga
     2881 gaccgcgctc cagcaaatgg gtagcgccct cttcatggcc ctctttcccg gacaggttcg
     2941 ccggttgtat gacgcagcgc gatccgaaca ggtcagccag cggctcaatc tgattttcac
     3001 ctccgacatc ggctggattg ccgatcagtc gtgggaattc atttatgacc cagaccggcg
     3061 cacgttcctc gcattggaag aagtcaattt cacgcgcaat gtgctgacgg ccattcccgc
     3121 cgaacgtatt ccggccagac ccacgatgcg gatcctcgtc gtggtcgcgc agcctttggg
     3181 actcggcgcc ctgtcggtgg aagaagaggc cgatgtgatc cgcagcggat tccgccgcct
     3241 gctcgatgcg ggactggccg aggttgaact cctgctcgac gccacgccgg aattgttgca
     3301 ccgggcgctg gagtctgcgg ccgccccgat cgacgtgctg cacttcatcg gacacgggga
     3361 atacaacgag cagaccgact gcggatactt gatcttcgag aatcaagacg gcggcgaaca
     3421 gcgattggac tcctccacgc tccgtcagat cgtctgccgg cgcgggatac gtctggtctg
     3481 cctgaacgcc tgcgaaaccg gccgcggcgg gcgacaggat ttcagccgcg gtgtggcgca
     3541 ggcgctgatc gccggcggcg tccctgccgt ggtcgccaat caatatccgg tgttggatgt
     3601 gtcggccact gccttctccc gccacttcta ctgggccctg gccatgggac atcccatcgg
     3661 cgatgcggcc cgcgaggcgc gcgtgtcggt caactatgcg atctccggag aagccatcga
     3721 ctgggcagtg ccggtcgtct ttgcgcgtaa tcccgcacaa cggctctgcg tgccgcgcac
     3781 cgctgccggg tatgagcgga gccgcagggc ggtggtacgc cagcggcggc gcgcggtaga
     3841 agaccgcatc aagatcggca tctggaacgc gcatcgcatg atcccgcacc tgcccgacat
     3901 ctgcgagcgt ctcaccaaag cccaggatcg gtacgccttc gagaccgtat ccttccctgc
     3961 tcccatcggc atctggcgcc gggagcagga gaaggatcag gcatttgtgg tcgccgaaac
     4021 gttgtacgac cggttgaagg gcaaaccgaa agagttgggc gtggagacgc tcatctgcat
     4081 gatcaacttt ccgatgaagg acgcgcgaac gagatacctg tattactgga ggcgagaccc
     4141 gctgatcgtc acgtcgacct ttggattact ggaacaattg aacgagcggg agttcaccgt
     4201 tgagcgcatg atggctcatc tggcggcggc ggtcgtcgca ggaatcccgc cgcatccccg
     4261 gcgggtggga cctgctgact gtcccctctt ctacaatgaa cagcgggaca ttcgtttgat
     4321 cgcgggccgg ctcacgttct gtgccaactg tcgaaaacaa ttcaaagaca aaccggcgca
     4381 ggaacgcctg cgggctgccg agcgacttct tgccgcctat ccgtagacgg cacattggtc
     4441 attcggactt gtgacactcc cgcacggcct tgagcaaatt ctccggcgtg ccgatgtcaa
     4501 ggtagcgcgc gccggggact ttcacgctct atgtataagc ctgcatcgtt ggcaaatgag
     4561 cccctgagca gcgcgatttt gagtgggaaa tgaaaactcc gggagtctga atttccgcga
     4621 cgggagggac cattagcggt agattattgc tgtgtctacc aagattaaga cattcgtcct
     4681 ctgctgcagt actcttgtac tgacggcctg cccacaacct cacgctgttc cgcctccctc
     4741 gtttccgccg tcatgggaaa cgctcattcg caaaggcgac atcgaacagg agtattggcg
     4801 atatcctgaa gccgaatact actattccca agccgtgaca cgcgcccagg aattcggagc
     4861 cgatgacgct cgcctggcaa ggtcgctacg aagcctgtct tccgttgtct caagccgagg
     4921 aaactatgct gcagctaagc agtatgggga gcaggcactt ggcatatatc gacatctctt
     4981 cgggccggag agcggtgaag ttggcatgac cgaatacata ctgggcagca ttgccgcaga
     5041 gcacggctca ttcagcgaag ccaaacaact ctacatgcaa tcgcagaggc acatcgctgc
     5101 ggcttacggc cccgaagatc ctcgcctagg ccagccgatg atagacttgg ccgggctata
     5161 tctgatgacc aatcgcatga tcgaagcaga aaatctgctt cgacaggtca tcacactgtc
     5221 ggacaaacct gagaaccgcg atccatccag actgagtctg gctcttcgcc atctcggcgc
     5281 cctcctcctc acgcaaaagc gattggacga agc
//
LOCUS       DAOQEW010000236         5262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6278_length_5262_cov_10.171692, whole genome shotgun sequence.
ACCESSION   DAOQEW010000236 DAOQEW010000000
VERSION     DAOQEW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6278_length_5262_cov_10.171692"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            502..1671
                     /locus_tag="PLS35_17410"
     CDS             502..1671
                     /locus_tag="PLS35_17410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20504.1"
                     /translation="MYSSTPRQRCAFTILIALSAIFLIGCNTGSNTPVESPPLDVAVA
                     NVLVRSVQTWDEFNGRVHAIPTVELRPRVSGYINRVAYKEDSEVKPGDLLFVIDPRPY
                     RNALHSAQAQLERARSTANLAQIKTEHAQALFETRAMSREDFHGRQTELNQTAAEIRA
                     AEAAVATAKLNLDFTEVRAPIAGRVGRAYLTVGNLAQADQSILTTLVSQDPMYVYFDC
                     DEHSFLRYKEFARRNDRGNNAYLVRIRLANEEGFPHHGTVDFFDNQVNPTTGTIRARA
                     VVSNTDRFLTPGLFARVQLQGDELLALLIDTKAILTDQHHKYIYVLGEGNKALRKNIT
                     LGRQIDDLYIVQSGLDKTDMVIVTGAQKIIREGMIVRPEYVAMEAREAISPLPVQ"
     gene            1696..4824
                     /locus_tag="PLS35_17415"
     CDS             1696..4824
                     /locus_tag="PLS35_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNI20505.1"
                     /translation="MDFSKFFIDRPIFAIVLSIVIFAGGLIAIPLLPAGEYPEVVPPS
                     VVVRAIYPGANPKEMADSIAVPLEEAINAVEGIMYMKSVAGSDGSLQVTVTFTPGIDP
                     DTATVRVQNRVAQALSRLPPDVRLYGVTTQKQSPTPLMFVSLTSPDNRYDGVYLRNYL
                     TLNIKDQLSRLQGVGNVGLYGEGDYAMRLWLNPGKLATRGLTASDVINAVREQNVQVS
                     AGQLGAQPSPKSTDFLVPINVRGRLRSEQEFSDIVLKSGDHGQLVRLSDVGRVELGSG
                     DYTLHAMIDGKNGASAGIFLTPNANSLAVARAVYAKFEELSANFPAGVTYRIVWDPTV
                     FVRESIRAVGQTLIEAVFLVVLVVILFLQTWRASIIPLIAVPVSIVGTFAWLYLLGYS
                     INTLTLFGLVLAVGIVVDDAIVVVENVERYIEEGLDPLAAAHQAMKEVSGPIIAIALV
                     LCAVFVPMAFLSGVTGEFYKQFAITIAISTIISTINSLTLSPALAAKLLKRHDAPKDA
                     LTRLLERMLGWLFHPFNRLFLRSSHKYHNTVARTLGRRGIAFTVYAGLLLSTAILFHI
                     VPTGFIPNQDKLYLFAGAKLPEGASLARTNAVTHQLNKIASTIEGVDITESYVGLNAL
                     QSVNTPNQVTSYIILKPFDQRQRSAESITAELNAKLVEVKDGQAYALLPPPIQGLGNG
                     SGYSLYLVDRAGLGYGALQEALTTFQNAIAQTPGMTFPVSSYQANIPQLEVNIDRVKV
                     LAQGVLLSDLFNTLQIYLGSIYINDFNLFGRVYRVLAQADSVFRQKTEDISNLYTRNV
                     QGEMVPLGSMVTIQPSFGPDPVIRYNGYPAADLIGDADPRVLSSGQVITKLSSVAAEV
                     LPRGITLEWTDLSYQQVTQGNAAVIVFPLSIMLVFLVLAALYESWTLPLAVILIVPVC
                     MCAALFGIWLTGGENNIFVQVGLVVLMGLACKNAILIVEFARELEIQGKSTVEAALEA
                     CRLRLRPIIMTSIAFIAGSVPLVLSYGAGSEVRYATGITVFSGMIGVTLFGLFLTPVF
                     YVALRKLARLKP"
     gene            complement(4877..5262)
                     /locus_tag="PLS35_17420"
                     /pseudo
     CDS             complement(4877..5262)
                     /locus_tag="PLS35_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1224 a         1448 c         1278 g         1312 t
ORIGIN      
        1 gaaaaggtgg tttatttttc ctatacccaa tccgtctagc tttatatata gttctgctat
       61 agaatttcta tctcgttgta atttattaaa gggaattcag atatcaatac ttacacagtt
      121 gacctggagc aaataatttt tttcattgac aagatggcaa agccgctaga agatggtggt
      181 gtctacttca gaccatccct agatcaggta gcgccagatt tcttgcctgc aaccattacg
      241 gcttgaagcc atctgttaga tcaagttcaa gtcgggataa tttagtcttt ctccgatgca
      301 ttctttctcg gaaatcctct actaggaata tttgagacct ctgcctaact ggttttttgg
      361 ataatttgtc atttttaaac gattgatttt aaaaattacc gcatgagcaa aaaaccttaa
      421 tgacataggt atgcagaggt ttcatttaga atgattgagt cttacgaccc tccccgccag
      481 aaatttttca tgatttaaca tatgtacagc tcaaccccac gccaacggtg cgcatttaca
      541 attcttattg cgctctcggc aatcttcctc atcggatgca ataccggatc caacactcca
      601 gtcgaatcac caccgctcga tgttgcggtt gcgaacgtac tggtgcgttc agtgcaaact
      661 tgggatgaat ttaatggccg tgtccatgca atcccgactg tagaattgcg tcctcgcgtg
      721 agtggttaca tcaatcgtgt tgcctataaa gaggatagcg aggtcaaacc tggcgacctt
      781 ctcttcgtca ttgatcctcg gccgtaccgc aatgcactcc atagtgcgca ggcgcagctc
      841 gaacgtgccc ggtcgaccgc gaatcttgcg caaatcaaga ctgagcacgc gcaggcgctg
      901 ttcgaaacac gggccatgtc acgcgaagat ttccacggta gacaaacgga gttgaaccaa
      961 acggctgccg agattcgtgc ggctgaagct gctgtagcca ccgccaagct caacctcgat
     1021 ttcaccgagg ttcgagcgcc aatcgcaggc cgcgtggggc gagcatacct aaccgtcggc
     1081 aatcttgccc aggctgacca atcaattctg acaaccttgg tctcgcagga tcccatgtat
     1141 gtatacttcg attgcgatga gcatagcttc ctgcgctaca aggaattcgc tcgcaggaac
     1201 gatcgcggga acaatgcata tctggtccgc atccgtctgg ctaatgagga aggcttcccg
     1261 caccatggga ctgtagattt cttcgacaat caggttaatc ccacaacggg cacaattcgt
     1321 gctcgcgctg tcgtctccaa taccgaccgt ttcctgacac cggggctttt cgcccgtgta
     1381 cagctgcaag gcgatgagtt acttgcactt ttgatagaca ccaaggccat tctgaccgac
     1441 cagcatcaca aatatattta tgtgctagga gaaggaaaca aggcactacg caaaaatatc
     1501 acattgggtc gtcaaatcga tgacctatac atcgtgcagt ccggcctcga caagacagac
     1561 atggtcattg tcaccggcgc acagaagatc attcgtgaag gaatgattgt aaggcctgag
     1621 tatgtcgcta tggaagcacg agaggctatc tccccccttc cggttcaata agagccgcgg
     1681 aataagagcg cgatcatgga tttttccaag ttcttcatcg accgccccat atttgcaatt
     1741 gttctttcga tcgttatttt cgcgggggga cttatcgcca ttccgctcct acccgcgggc
     1801 gagtacccag aggtggtgcc gcctagtgtc gttgtgcgag caatataccc cggcgccaat
     1861 cccaaagaaa tggcggattc aatcgccgtt ccgttggaag aagcgatcaa cgccgtcgaa
     1921 ggcatcatgt acatgaagtc cgttgcgggt tccgacggaa gcctgcaagt gacagtcacc
     1981 ttcacccctg gcatcgatcc cgataccgca actgtccgtg tacagaatcg cgtcgcccag
     2041 gccctgtcgc gtctgccccc cgatgtgcgt ctgtacggcg taaccacgca gaagcaatcc
     2101 cccactccgc tcatgtttgt gagtctgact tcaccagaca atcgctacga tggggtctat
     2161 ctacgcaact acctgaccct gaatatcaag gatcagctct cgcgcctgca gggtgttggc
     2221 aacgtcggat tgtacggaga aggcgactat gcaatgcgct tgtggctgaa cccgggcaag
     2281 cttgccacac gcgggcttac agccagtgat gtgatcaatg ccgttcgaga gcagaacgtc
     2341 caggtatccg cgggtcagct gggcgcgcaa ccgtcaccca agagtacgga tttcctcgtt
     2401 ccgataaatg tgcgcgggcg gcttcgcagc gaacaggaat tcagcgatat cgtgctgaaa
     2461 agcggcgacc atggccaact ggttcgctta tccgacgtgg gacgcgttga gctcggatcc
     2521 ggggattata cgctgcacgc catgattgat ggaaagaacg gcgccagcgc gggcattttc
     2581 ctcactccca acgcaaactc cctggcagta gccagagcgg tctacgccaa gtttgaagaa
     2641 ctttcagcaa acttccctgc gggcgtgacg tatcgaatag tgtgggaccc taccgtattt
     2701 gtccgggaat ccatccgggc ggtgggtcaa accctgatcg aagcggtttt cctggtcgtg
     2761 ctggtggtca tcctgtttct gcagacctgg cgtgcatcca tcattcctct gatcgcggta
     2821 ccggtctcga ttgtcggtac cttcgcttgg ctgtaccttc tcggatattc catcaatacg
     2881 ctaacattgt ttggactggt gttggcagtc ggcattgtcg tcgacgatgc catcgtcgtc
     2941 gtcgagaacg tcgagcgcta catcgaggaa ggcctggatc ctcttgcagc agcccaccag
     3001 gccatgaagg aagtctccgg ccccattatt gccatagccc tggtgctttg cgccgtgttt
     3061 gtgcccatgg cgttcctaag cggtgtcacc ggcgaattct acaagcaatt tgctatcacc
     3121 atcgccatat ccaccatcat ttccacaatc aattcactga cattgtcgcc ggccctggcg
     3181 gcaaaactgc tcaaaagaca tgatgctccg aaggatgcgc tgacacggtt gctcgagcgt
     3241 atgttggggt ggctgttcca tccgttcaac cggctatttc ttcgcagctc ccataaatat
     3301 cataataccg tggctcgcac gctgggccgt cgtggcatag cgttcaccgt ctatgcaggg
     3361 ctattgctct cgaccgcaat actgttccat atcgtaccga ccggctttat tcccaaccag
     3421 gataaactct accttttcgc gggagccaaa ttgccggagg gcgcttcgct tgcccgaact
     3481 aatgctgtca cacatcaact gaacaaaatt gccagcacta tcgaaggcgt agacataacc
     3541 gaaagctatg tgggcttgaa tgccttgcaa tccgtcaata ctcccaatca ggtcacctcc
     3601 tatatcatcc tcaaaccttt cgatcagcgc cagcgttctg cggaatccat caccgccgaa
     3661 ctgaatgcca agcttgtgga ggtcaaggac gggcaggctt atgcattact gcccccgcct
     3721 attcaaggac tgggtaatgg gtcgggctat tctctttatt tagttgatcg cgccggcttg
     3781 ggatatggag ctttacaaga ggcattaacg actttccaaa acgcaatcgc ccaaacgcca
     3841 ggcatgacct ttcctgtcag ctcatatcaa gccaatatcc cgcaactcga ggtcaatata
     3901 gatcgagtta aagtattagc ccaaggagta ttattgtcag atctatttaa taccctgcag
     3961 atttatctag gttctatcta tattaacgat ttcaacttat tcggtcgggt ctatcgagtg
     4021 ttggctcagg cggactcagt ttttcgccag aagaccgaag acatcagcaa tctgtacacg
     4081 cgcaacgttc aaggggaaat ggttccgcta gggtcgatgg tgacgattca accttcattt
     4141 ggcccggatc cagtcatccg ctacaacggc tatcccgccg ctgaccttat cggcgatgct
     4201 gatccgcgcg tactttcctc ggggcaggtc atcaccaagc tctcgagcgt tgccgcagag
     4261 gtgttgcccc gcggcattac gctggagtgg accgatctta gctaccaaca ggtaacgcag
     4321 ggcaacgcgg cggtcatcgt attcccatta tcgattatgc tggtctttct ggtgctagcc
     4381 gccctctacg aaagctggac attacctttg gcggtcatcc ttatcgtgcc ggtatgtatg
     4441 tgcgctgcgc ttttcggtat ttggctcact ggcggcgaga acaatatatt cgttcaggtg
     4501 ggactggtcg tgctgatggg cctggcatgc aagaatgcca tcctgatcgt tgagttcgca
     4561 cgggagttgg agatccaggg caagagtact gtcgaagccg cactggaagc ctgccgtctt
     4621 cgacttcgac cgatcataat gacctcaatc gctttcatcg cgggatctgt tcccttagtg
     4681 ctcagctacg gtgcaggcag tgaggtgcgc tacgcaacag gtataaccgt tttttccggg
     4741 atgatcggcg tgacgctatt cggattgttc cttacccctg tcttctatgt cgctttgcgc
     4801 aagctggctc gtctcaaacc ttaggggcga attagcctca gatcaatatc caaaacttcc
     4861 gcacatcatg tgaaaccgac tttctatcac tgtgatgaat caatcctgag gttccccgcc
     4921 gtgcccatag cgcctgttcc aatgcatcca gtatcagttc cgttcgcaat gaacgactca
     4981 ctcgccagcc aacaatatac cgggcaaaaa catcggtgat aaaagccaca taaacaaatc
     5041 ctgtccatgt cgctacaaat gtaatatccg ccacccacaa ttgattgggc cgggcagcta
     5101 caaattgccg gttaaccttg tccgttggcc ggtcaagcaa atcgtccgaa atggttgtcc
     5161 aacacttttt gccgcgtcga acaccttgta ttccaagctt cttcattaac cgctcgaccg
     5221 tacaacgagc aacaccaata ccttctcgca gcaactgccg cc
//
LOCUS       DAOQEW010000237         5258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6286_length_5258_cov_9.206804, whole genome shotgun sequence.
ACCESSION   DAOQEW010000237 DAOQEW010000000
VERSION     DAOQEW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6286_length_5258_cov_9.206804"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            670..1308
                     /locus_tag="PLS35_17425"
     CDS             670..1308
                     /locus_tag="PLS35_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNI20506.1"
                     /translation="MNVLARILHPTDMPMVGTFRRLYRSVQDLAGQIYEDWATNNHDW
                     TMPGFRAIAVHRFGTWVSNKRPGALRTVLLWLYQVMYRYIRNYYGIEIPLTVILGRRL
                     WLVHQNGIVFHWKTVVGDDCLIRHNATIGAGYGGQKTLHKGPRLGNHVEVGPGAVIFA
                     AVAIGDGAVIGPNVVVMSNVPAGARVFAEAPRLIRLANAPDAHIVEGMSQNR"
     gene            1320..3326
                     /locus_tag="PLS35_17430"
     CDS             1320..3326
                     /locus_tag="PLS35_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNI20507.1"
                     /translation="MTPTGIVSPQQLTSSDTPETRGDAYRKQGQLEQAIRCYLEAVTF
                     PLSPALCLKLARCYDRLDQHAEACRWALSLVEAGDDYTTWLAGWTLFQRHAGQGGWSA
                     LRSTKVAMVSSYTTTQFCPLLQLAAARVGIHIDLYESQYGQYQQDIIDPGSRLYAFAP
                     DIVILAVHEGDLRLPEYSSTPDQDIQAEVDRWTTLWKLVAERAQARVVQHNFALPCEV
                     PAGHLATRLPGSRYRMTQAVNMRLGDSAGLGVSLVDCERLSGLIGKRQWCDPRYWNLS
                     KQAVALEALPLLARHTAAVIAADLGLSRKCLVLDLDNTLWGGVIAEEGLAGIKLGQGP
                     EGEAFVAFQEYLLKLKRKGVILTVCSKNNHADAIHPFEKHPEMRLRLQDIALFIANWE
                     SKPDNIRRIADTLQIGLDSLVFVDDNPVERDIVRKFLPEVDVLPLPDDPAYYTRALSQ
                     YLSLETASLTAEDSERADQYRARAQIKELEAAAGSIEDFYLSLRMQAIVTPFEESQLP
                     RIAQLIGKTNQFNLTTRRHGMPQLTAFIQSTDYVHLALRLRDRYTDHGLVSVMIAQCR
                     GEVLDIDTWLMSCRVIGRTVEATMLELLCRHAAQLGCTILQGTYIPTQKNAMAADVYA
                     KHGFEPFNQTNGHHVWRYDLTANGPIANPFVKRVDAWESVDGTP"
     gene            3361..4860
                     /locus_tag="PLS35_17435"
     CDS             3361..4860
                     /locus_tag="PLS35_17435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNI20508.1"
                     /translation="MITVRFTKQDLACFSAASRDRNPLHISEDYARTTPYAEPVVFGV
                     LSLLAGLGHLSTRHGRQLQHLSVEFRNPLSLDVPYRLDIIESSTDNARVKLYDTTRLM
                     MTATFAFMPGQGNPESRRFSETSCATEPEDRKKDSLLPGTRVTGTYAPHTEAFAQVVD
                     RWELEGKGASLYQLAAMMWASYVVGMHLPGKRAVLWRLTLDFHDVEPPGQEPFSYEAT
                     VEDLDERYDLLRCRGTLSAGSLPYATAHMSAFVRQDSPRSSLRRISDLLPESEQLKGK
                     RALIIGGSRGLGAAIAQALISQGCSVLLSYQQSTAEAEQLRASVGDRSTQLELVQGDA
                     ADIEWCLSLRDMILSRYGGLDLLVCNASPPIRPLAFEPEKFTQFQDFLTKSLALVSAP
                     MSTFLGLLAHQAGWNIILSSSYVKELPAAFAHYVTAKCALEGLVNWAAVQYPTVRHRI
                     VRPPKLLTDQTNTTIGRQGAMDVEQVAVSIVRQICQPHQPAAVQIMETF"
     gene            4946..5212
                     /locus_tag="PLS35_17440"
     CDS             4946..5212
                     /locus_tag="PLS35_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNI20509.1"
                     /translation="MELHDRLEEVFRQVFDNEALELRDQMKAADIEGWDSVAHINLMF
                     GIEQAFGIRFKGNELADMKNIGELKDFLAGKLNGEASPMRKVLP"
BASE COUNT         1136 a         1565 c         1462 g         1095 t
ORIGIN      
        1 gcaacatcga gtcaggcctg aactatttca acgcatccct caccaggccc cagcgggaga
       61 ccgcgaatag taccacgggc cccattgcca tacaagccat gaaataggca tttcggccca
      121 gagttgacgg acgactgtcc aatatgatga cccgctctcg cttgccctcc gtttgcgccg
      181 ttcatcagca gctgacacgc cacagtctgc cggcactagg gatataccta gtcgatttcc
      241 gcttctgttt gtccaatgct gataaacagg ggtccgccac cctggaatgc tccgcaaccg
      301 actccgtctc tcacctcctc gcgtggttca ttgtcgtcgc gagatctaca caaacatcct
      361 ttcatcatca cgaatttgtt gatcgacggc gttttgggct tgtccagtac tgctggacat
      421 atgataagat tggctacatt caccagccgc cggaagaacg tggctgatcg agggacatcg
      481 atctgtgtga gcagcaccgt tcactattga tcccgacgat catcatgacc gaccaacaag
      541 actgctgatc cgcagtccga cgatggaaga agtgattgcc gcggaagggt cgagtgtgcg
      601 agctagccca tctgcccaca gcactgcctt ggactgttga agaatggcag tgcgacgaaa
      661 tgaggcgcta tgaacgttct ggcgagaatc cttcatccaa ccgacatgcc catggtagga
      721 acgttccgca gactctatcg cagcgtgcag gatctggcgg gccagattta cgaagactgg
      781 gccaccaaca accatgactg gacgatgccc ggatttcgcg ccatcgccgt ccatcgcttt
      841 ggcacctggg tttccaataa acgacccggg gcgcttcgga ccgtactgct ctggttgtac
      901 caggtcatgt accgctacat acggaactac tacggcattg agattcctct gactgtcatc
      961 ctggggcgaa gactgtggct ggtgcatcaa aatggcatcg tgtttcattg gaagacggtt
     1021 gtcggtgacg actgtctgat ccgtcacaat gccaccatcg gcgccggtta tggcggacag
     1081 aaaacattgc acaagggccc caggctcggg aaccacgtgg aagtaggtcc cggcgcggtg
     1141 atttttgccg ccgtggccat tggagacggc gcagtgattg gcccgaacgt ggtggtgatg
     1201 tcgaatgttc ccgcaggagc ccgagtcttt gccgaagctc cccgtctgat acggctggcc
     1261 aatgcccccg acgcccacat cgtcgaggga atgtctcaaa acaggtgagc cagcagagca
     1321 tgacccctac cggcatcgtc tcgccacaac agctcacgtc ttccgacacg cccgagacac
     1381 gcggtgacgc ctatcgcaag caaggccaac tggaacaggc cattcgctgc tatctcgagg
     1441 cggtgacctt tcccctctct cccgcgctct gcttgaaact cgcgcgatgt tacgatcgct
     1501 tggatcagca tgctgaggcc tgtcgatggg ccctttccct ggttgaagcg ggtgatgatt
     1561 acacgacctg gctcgcaggc tggacgctct tccaacgtca tgccggccaa ggtggatggt
     1621 ctgcattgcg atcaaccaag gtcgccatgg tgagtagcta cactacgact caattctgcc
     1681 cgttattaca gcttgccgct gccagggtcg gcattcatat cgatctctac gagagccagt
     1741 acggacaata ccagcaggac atcatcgatc cggggagccg cctgtacgcg ttcgctcccg
     1801 atatcgtcat cctcgcggtt cacgaaggag atctccggct ccccgagtac agctcaacgc
     1861 ctgaccagga cattcaagct gaggtcgacc gttggacgac actctggaag ctggtcgcgg
     1921 aacgggcgca agcccgcgtc gtacagcata actttgcgct gccctgcgag gtgcccgccg
     1981 gccatctcgc gaccagactt cccggctctc gctacaggat gacgcaggcc gtgaatatgc
     2041 ggttaggaga ttcggcagga cttggcgtgt ccctggtgga ttgcgaacgt ctgtcggggc
     2101 tcatcggaaa acggcagtgg tgcgatccgc gatactggaa cctttccaaa caggctgtgg
     2161 cgctcgaggc gctaccgttg ctggcacgac acacagcggc tgtgatcgcc gcggaccttg
     2221 gcttgagccg taagtgcctc gtactcgatc tcgacaacac gctatggggc ggagtgatcg
     2281 cggaagaagg tctcgccgga atcaaattgg gacagggacc ggaaggcgag gcattcgtgg
     2341 cgtttcagga atatctcctc aagctgaaac ggaaaggcgt catcctgacc gtctgttcaa
     2401 agaataacca tgccgatgcc attcatccgt tcgaaaaaca tcctgagatg cggctcaggc
     2461 tgcaggacat cgcccttttc atcgcgaatt gggaatccaa gccggacaac atccgccgga
     2521 tcgccgacac cctacagatc gggctggata gtctcgtgtt tgtcgacgat aacccggtcg
     2581 aacgtgacat tgtccgcaaa tttcttcccg aggtcgatgt ccttccgttg cctgacgatc
     2641 cggcctatta caccagggcc ctgtcgcagt acctgtcgct cgaaaccgcc tctttgacgg
     2701 ccgaggatag tgagcgagcc gaccagtatc gagccagggc gcagatcaaa gagttggagg
     2761 ctgcggccgg gtcgatcgag gatttctatc tcagcctccg catgcaggcg atcgtgacgc
     2821 ccttcgaaga atcacagctg ccgagaatcg cgcaactgat cggcaagacc aaccaattca
     2881 atctgaccac cagacgacac ggcatgccgc agttgaccgc cttcattcag agcaccgact
     2941 acgtgcacct cgccttgcgg ctgcgggatc gctacaccga tcatggcctg gtcagtgtca
     3001 tgattgcgca atgccggggc gaggttctgg atatcgacac ctggctcatg agttgccggg
     3061 tcatcggccg caccgtggaa gcgaccatgc tggaactcct ctgccggcat gccgcccagc
     3121 tcggctgcac tatcctccaa ggaacgtata tcccgaccca gaagaacgcg atggcagcag
     3181 atgtctatgc gaaacacggt ttcgagccct tcaaccagac caatgggcat cacgtgtggc
     3241 ggtatgacct gacagcgaac ggcccgattg ccaatccgtt tgtgaaacgc gtcgatgcat
     3301 gggaatccgt tgatggcacg ccctgacttg tcgagttcga ctcaatgctt cctgcctcat
     3361 atgataaccg tccgctttac gaaacaggat cttgcatgct tcagcgcggc gagtcgcgac
     3421 agaaaccctc tccacatttc cgaggactat gcccgcacga cgccttatgc cgaaccagtg
     3481 gtgttcggcg tgttgagtct cctggcggga ttgggtcatc tttcgacacg ccatggcagg
     3541 cagctgcagc atctctcggt cgaatttcgt aatcccctca gcctcgacgt gccctatcgt
     3601 ctcgacatca tcgaatcgtc gacagacaac gcacgcgtca aactctatga tacgactcgt
     3661 ctgatgatga cggcgacatt cgccttcatg ccaggacagg gcaatcctga atcacggcgc
     3721 ttctcggaga catcttgcgc tacggagccg gaagatcgaa aaaaagactc cctccttccg
     3781 ggcactcgcg tgaccggcac ctatgcgcca cacaccgaag cgttcgcgca ggtggtcgac
     3841 cgctgggagc tcgagggaaa aggcgcgagc ctataccagc ttgcggccat gatgtgggcg
     3901 agttatgtcg taggcatgca ccttcccggc aaacgcgcgg tgctctggcg gctgacgctt
     3961 gattttcacg atgtagaacc accaggccaa gaaccgttct cctacgaggc aaccgttgaa
     4021 gacctcgatg agcgatatga tctcttgcga tgtcggggca ccctttctgc cggctcgctc
     4081 ccctatgcga cggctcacat gtcggcattc gtgcgacagg attctcctcg atcttcgctt
     4141 cgacgcatca gcgacctgct accggaatcc gaacaattga agggaaagcg cgcgctgatc
     4201 atcgggggaa gccggggtct tggcgccgcc atcgcgcaag cgctcatttc acagggctgc
     4261 tccgtgttgc tgagctacca gcagagtacg gcggaagcgg agcaacttcg cgccagtgtg
     4321 ggtgaccggt cgactcagct tgaactggtg cagggcgacg ccgcagatat tgaatggtgc
     4381 ctgtctttac gggacatgat tctctcacgg tacggaggcc tcgaccttct cgtctgcaat
     4441 gcctcgcccc cgattcgtcc gcttgcattc gaaccggaaa aattcacgca gttccaggac
     4501 ttcctcacga aaagtctggc actggtctcc gcgccgatgt ccacgtttct cggcttgctg
     4561 gctcatcagg caggatggaa catcatcctg tcgtcatcct atgtgaaaga gcttccggca
     4621 gccttcgcgc attacgtgac ggcaaaatgc gcgctggaag gcttggtgaa ctgggctgcc
     4681 gtccaatacc cgaccgttcg gcatcgtatt gtcaggccgc ccaaactttt gaccgatcag
     4741 accaacacaa ccattggacg gcagggagcc atggacgtcg aacaggtcgc ggtctccatt
     4801 gtgcgacaga tctgtcagcc ccaccaacca gcggccgtgc agatcatgga gacattttga
     4861 cgggccatga caacgaccta acaggcacaa gtcttttacc cgaccaatga ccatacgaca
     4921 cctaaccgca tgatgtgagg agttcatgga gctgcacgat cgattggaag aagtctttcg
     4981 ccaagtattc gacaatgagg cgcttgagct gcgcgatcag atgaaagccg ccgatatcga
     5041 aggctgggat tccgtggcgc atatcaatct gatgttcggc atcgagcagg cgttcggaat
     5101 ccgatttaaa ggcaatgagt tggccgacat gaagaatatc ggcgagctga aggatttttt
     5161 ggccggcaag ttgaacggcg aagcgtcccc catgcgaaag gttctcccct aaggcgcatc
     5221 tgcggagcat gccacatgca gctagcgatc gctgcctc
//
LOCUS       DAOQEW010000238         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6333_length_5231_cov_5.990726, whole genome shotgun sequence.
ACCESSION   DAOQEW010000238 DAOQEW010000000
VERSION     DAOQEW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6333_length_5231_cov_5.990726"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLS35_17445"
     CDS             <1..277
                     /locus_tag="PLS35_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20510.1"
                     /translation="EFIRSLYRLFWVDGLDLSNERLLRLEAERQGFVGTALFGSTAPA
                     VESMLGEWRDRWEETEHQGVPLLQRADGQVLVGLVPPETLTQFLASS"
     gene            437..1186
                     /locus_tag="PLS35_17450"
     CDS             437..1186
                     /locus_tag="PLS35_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI20511.1"
                     /translation="MKSRFWSLGVVVLAGVLLQVYPALLLAADPAANAVKSPSASAEG
                     KAEETKSELYISLYMQGSRPLNRPARLQEDPFTNTDVQGGLGGGLKIGFFPAFAGRYV
                     GLEAELSGLDGKVNAPTATSGGVTRSANFRLQSINAMANLLLRYPGDVIQPYIGIGAG
                     LSGGFARDLNLQHSTIGTVHENAADGAFAYQFLGGVRANITDRLFLFTEYKYFVANYQ
                     WESEFTNGSRGPSFTLNYRTHIIAGGFGVTF"
     gene            1330..>2315
                     /gene="clpX"
                     /locus_tag="PLS35_17455"
     CDS             1330..>2315
                     /gene="clpX"
                     /locus_tag="PLS35_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNI20512.1"
                     /translation="MFWDTRHSTQKPDRRCSFCGKPQDAGRTLVTNSEPFACTSCRNP
                     AAVSICEECIQRCSLSLAKNKTPGSQSSVSPLPLVIPKPTDIKAKLDEYVIGQDRAKK
                     VLSVAVHNHYKRIVNRERLKHVDLEKGNILMIGSTGTGKTLLSQTLARILHVPFTIAD
                     ATALTEAGYVGEDVENVVLKLLQNCDYDVARAETGIIYIDEIDKISRKSENPSLTRDV
                     SGEGVQQALLKLVEGTVCNVPPKGGRKHPEQDYIRVDTTNILFIAGGAFVGLDAMIAQ
                     RTMPKRLGFGAIAPAGELEGHSEVLRCVQSEDLLKFGLIPEFVGRFPVLTTLA"
     assembly_gap    2316..2415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2416..2768
                     /locus_tag="PLS35_17460"
     CDS             <2416..2768
                     /locus_tag="PLS35_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNI20513.1"
                     /translation="FGLIPEFVGRFPVLTTLADLDVPALIRVLSEPRNALVKQYQALF
                     EIEGIDLQFTEAAIKAVARRAAVMKTGARALRTILEEVMLDLMYDVPALSGVKSVVIT
                     EEVIAGIGQPKILA"
     gene            complement(2888..3049)
                     /locus_tag="PLS35_17465"
     CDS             complement(2888..3049)
                     /locus_tag="PLS35_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20514.1"
                     /translation="MEVEIIGAGTMIALVWLIAWSMAGESESERRRITSAPGTFDTAS
                     GETRVRHAA"
     gene            complement(3670..4077)
                     /locus_tag="PLS35_17470"
     CDS             complement(3670..4077)
                     /locus_tag="PLS35_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20515.1"
                     /translation="MRTWLIAIIFLAGPLLPAAGAPVGAAADRPTPKASELLDEIIPK
                     ELFRHLYSEALSTLHEYIEMEGKLPGESSTHQQGGEFRLKLFPHGKSRSQEHLSAEGS
                     FRVAPEADQPEFTLRFKSFKKASPPARTNDDVI"
     gene            complement(4168..>5231)
                     /locus_tag="PLS35_17475"
     CDS             complement(4168..>5231)
                     /locus_tag="PLS35_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20516.1"
                     /translation="ACLIVTEAEVEFAIGTPVTPGLRQNDRQCLYQAKRNPQETVVVE
                     LNAGPDGDRKTLFQDQRSKAGHQPVPGVGDGAFTLRSPIGGIQLTFLKSDALVTLSLT
                     SPKQANPQAAVTNLAKVAANRLGAPIRTTPASAAEPGITGTPSLWAGEWYGCMPVGMM
                     YGKGHLSLNERGTWSMTTAVVMPGTIIADRGRWEAESYQEILHGTYKVAGSDRFSTTG
                     ILSVTWDKLAKNQGPTKFDPLLWQSFTAIPRKVNVKRLPAVEPSLVGTWEGSSKFVDR
                     QEDFVWTITAANVSEFFKATSQTGRIEQEQDRFRLVVPQSKTPPLSVRVLAQDKMELT
                     DTSGTVSQWNRNEKLLSRC"
BASE COUNT         1039 a         1439 c         1526 g         1127 t
ORIGIN      
        1 cgagttcatc cggtcgttgt atcgtctgtt ctgggtagat ggtttggatc tttccaatga
       61 gcgtctgtta cggctggagg cggaacgcca ggggttcgtc ggaaccgcat tgttcggatc
      121 aaccgcgcct gcagtagagt cgatgctcgg ggaatggaga gatcgctggg aagagacaga
      181 acatcagggc gtgcccttgc tgcaacgggc tgatggacag gtattggtgg gtctggttcc
      241 acctgaaact ctcacacagt tcctcgccag ctcgtgacaa cccattcgtt acgtacgaag
      301 ttccaccata cgtacagaca gagagtacgg ctgccgaaac tgccgaatca gtttgtcgcg
      361 caccttgctc gtgatatgag tgagctatcg caggccgttc tgggcctgtc ttgccacgta
      421 ctcggaggag tcactcatga aatcacgatt ctggtctctg ggcgtcgttg ttctcgccgg
      481 tgtattgcta caggtctatc ccgctctgtt gctcgcagca gaccctgcgg cgaatgccgt
      541 gaaatcgccg tcggcatcag cggagggaaa ggcggaagag actaagtcgg aactgtacat
      601 cagcctctac atgcagggaa gccggccgct gaatcgtccc gcccgcttgc aagaagaccc
      661 gttcacgaat acggatgttc agggcgggtt aggcggtgga ctcaagatcg ggttttttcc
      721 ggcgtttgcc gggcggtatg tcgggttgga agcggagtta tcgggcctcg acggaaaagt
      781 gaacgcaccg acggcgacga gcgggggcgt gacgagatct gctaatttcc gtctgcaatc
      841 gattaacgct atggccaact tgttactgcg ttatccgggc gatgtgattc agccctatat
      901 cggaatcgga gcgggattgt caggtggatt cgccagagat ttgaaccttc agcacagcac
      961 gattggtacc gtgcatgaaa atgcggccga cggcgccttc gcgtaccaat ttctcggcgg
     1021 cgttcgtgcc aacatcacgg accgcctgtt cctcttcacg gagtacaaat atttcgtggc
     1081 gaattaccaa tgggagagtg aattcacgaa tggctcccga ggtccctctt tcaccctgaa
     1141 ctaccgtacc catatcattg ccggtgggtt tggcgtgacc ttctagcagg ctgtggtcat
     1201 cctccggcag gcgctcgtcg cgccgttcag cgctgagacc gtgattcctc catgcttgca
     1261 ggtcgccatc acagaggcat aagatgaggc atcgcatcca ggcacatctc gtagcatgga
     1321 ggaaacgcgg tgttctggga cacgcggcat tcgacgcaga aaccagaccg gcgctgttcg
     1381 ttttgcggca agcctcagga cgccggccgg acgctcgtga cgaattcaga gccgttcgcg
     1441 tgcacctctt gtaggaaccc ggcagcggtt tctatttgcg aagaatgtat tcaacgctgc
     1501 tccctttccc tggccaagaa caaaactcct ggttcccaat cctcagtctc accgcttccg
     1561 ctggtcatcc ccaagccgac ggacatcaag gccaagctcg atgaatatgt gatcgggcag
     1621 gaccgtgcca agaaagtcct ctccgtcgcc gtgcacaatc attacaagcg gattgtgaat
     1681 cgggaacggc tcaagcacgt cgatctcgaa aaggggaaca tcctcatgat cggctcgacc
     1741 gggaccggga agacgctgct gtcccagacc ctggcccgta tccttcatgt cccctttacc
     1801 attgccgatg cgacggcact gactgaggcc ggatatgtgg gagaggatgt tgagaatgtg
     1861 gtcctcaagc tgcttcaaaa ttgcgattac gacgttgccc gggccgaaac gggcatcatc
     1921 tatatcgatg agatcgacaa gatcagccgc aagtcggaga atccgtccct gacgagagat
     1981 gtctccggcg aaggtgtgca gcaggcgctc ttgaagctgg tcgaggggac ggtgtgcaat
     2041 gtgccgccca aaggcgggcg gaaacatccg gagcaggact acattcgggt ggatacgacg
     2101 aacattctat ttatcgcggg cggggcgttc gtcgggttgg atgccatgat cgctcaacgg
     2161 acgatgccca agcggttggg gttcggtgca atcgctcctg ccggtgaact cgagggccac
     2221 agtgaagtgc tgcgatgtgt gcaatccgaa gacctgctca aattcgggct cattccagaa
     2281 tttgtgggtc gattcccggt cctcacgacc ctggcnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnaattc gggctcattc cagaatttgt gggtcgattc ccggtcctca
     2461 cgaccctggc cgaccttgac gtgccggcgc tcattcgggt gttgagcgag ccacgaaatg
     2521 cgctggtgaa acaatatcag gctctctttg agatcgaagg catcgacctc caattcactg
     2581 aggctgccat caaggccgta gctcgccggg ctgcagtgat gaaaaccggc gcgcgagcgc
     2641 ttcgcacaat tctcgaagaa gtgatgctgg atctgatgta tgacgtgccg gcattaagtg
     2701 gcgtcaagtc cgtcgtcatc accgaagagg tgatcgccgg catcgggcag ccgaagattc
     2761 tcgcctgaca atcacatcca actatcgatt cttggtccgg caatcccgcg cacacaccat
     2821 tcctcaggcg ccgctcccgc caataggcgg gatacaccac gtatctcctg cacaaccgaa
     2881 gcttcgttta ggctgcgtgc ctcactctcg tttcgcccga ggccgtgtcg aacgtgccag
     2941 gcgccgaggt gatccgtcgt ctttccgact cgctctctcc agccatggac caggcaatca
     3001 gccacaccag cgcgatcatt gttccggcac cgatgatctc gacctccatg tggcacctcc
     3061 gttgttgaac aacagattgt tggccgggac gccgatggag ccctctgtgc gacacagcat
     3121 ccatggcgtc tctcatccac gaggctatca gacgtgcgac gccctcacgc tgcccgacgc
     3181 gagccggacg acgcggatcc gaccgcctga ttcgacgtat cgactccatc tgctgacgaa
     3241 ttggacgagc ctccctgtgc gccatacaga atgtctatac aggcgaagag agttcctatc
     3301 atcgccagga aacctgccac gattccccaa tagactcctg acataggcca cgctcccgcg
     3361 cgggtatcgc gcgagtcctc tgtgcacgtg tcgccgtacc agtgattaac gcttggtgaa
     3421 gagcgaaggt acgcgagcgc gtaagaatgt gttgtgtgga tgcttgtaac tcggcggtct
     3481 taagagaaga ggtttggtcg aggagcaggg tacacctacc atgttcatcc cactggatct
     3541 gcgtcccaag gaagaacatg gctccgacct ccaaacatga ggcggatcgt gtactagaaa
     3601 tgtattctat gccccgctca gaccttggtc aagggcaagg tcggcgacgc gtgcgtgtgg
     3661 gagtgggctt cagatcacgt cgtcattggt gcgtgcggga ggcgaggctt tcttgaagga
     3721 tttgaatcga agggtgaatt ccggttggtc ggcctcgggt gccacacgaa acgagccttc
     3781 cgcgctcagg tgttcttgtg atcgggattt gccgtgagga aacagtttga ggcgaaactc
     3841 tcccccctgt tgatgggtcg aactctcacc gggcagcttg ccttccatct cgatgtattc
     3901 gtgcaatgtg gagagggctt cgctgtagag gtggcgaaag agttcttttg gaatgatctc
     3961 gtccagcaac tcgcttgcct tgggggtggg gcggtctgcg gctgctccga caggagcccc
     4021 cgctgccggt agcagcggtc cggcgagaaa gattatcgcg atgagccatg ttcgcataga
     4081 gttagtctat cggaggcggg aaaagcaggt caagttgcga aaaagtctgc cggccctgga
     4141 agggatgggc cttgtcactg aagaggacta acagcgcgac agcagctttt cattccgatt
     4201 ccattgcgac accgtgccgc tcgtgtcggt cagttccatt ttatcctggg ccaggacccg
     4261 gacggagaga ggcggcgttt tactttgcgg aacgaccaat cggaaccggt cctgttcctg
     4321 ctcgatgcgg ccagtttgtg acgtggcttt gaagaactcg gagacgttgg ctgcggtgat
     4381 cgtccagaca aaatcttcct gccgatcgac aaacttggag gacccctccc atgtgcccac
     4441 cagggacggt tcgaccgccg gcagacgttt gacgttgacc ttcctgggaa tcgcagtgaa
     4501 cgattgccag aggagcggat cgaacttggt cggtccttga ttcttcgcca gcttgtccca
     4561 ggtcacgctt aaaataccgg tggtcgaaaa ccggtcgctg ccggctactt tgtaggtgcc
     4621 gtgaagaatt tcctgatacg attccgcctc ccaccgcccg cggtccgcga tgatggtgcc
     4681 tggcatgacg acggcggtcg tcatcgacca tgtgccgcgt tcattcagcg agagatgccc
     4741 tttgccgtac atcatgccga cgggcatgca gccgtaccat tcaccggccc acaatgaggg
     4801 ggttccggtg attcccggct ccgcggcact ggcgggagtg gtacggatcg gagcgcccag
     4861 tctgttggcg gcgacttttg ccaggttcgt gaccgctgct tgtggattcg cttgtttcgg
     4921 cgacgtgagt gacagcgtca ccagggcatc cgatttcaga aacgtcagct gaatccctcc
     4981 gatgggagaa cggagcgtaa acgcgccgtc cccgacgcct ggtaccggct gatggccggc
     5041 tttggagcgt tgatcctgga acagagtttt tcgatcccca tccggcccgg cattcagttc
     5101 tactacgacc gtctcttggg gattgcgttt cgcctgatag agacattgcc gatcattctg
     5161 acgcagaccg ggggtaaccg gcgtgccgat cgcgaattca acctcggctt cggtcacgat
     5221 gaggcaggca t
//
LOCUS       DAOQEW010000239         5194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6428_length_5194_cov_8.669002, whole genome shotgun sequence.
ACCESSION   DAOQEW010000239 DAOQEW010000000
VERSION     DAOQEW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_5194_cov_8.669002"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..646)
                     /locus_tag="PLS35_17480"
     CDS             complement(173..646)
                     /locus_tag="PLS35_17480"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNI20517.1"
                     /translation="MVNENFRNNERVIVDDESLASNLFQRAQPFLPAVLDGHHLVGFN
                     ERWRFYRYQPGQTFQPHRDGSYLRMKTGDKSEVTMMIYLNDGMVGGETKFYASADHAF
                     LQPPAPYLSVRPKMGMALVFLHAIWHEGAVVQSGLKYVLRTDVLYGLHSAESRQG"
     gene            complement(860..3061)
                     /locus_tag="PLS35_17485"
     CDS             complement(860..3061)
                     /locus_tag="PLS35_17485"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNI20518.1"
                     /translation="MPMDQAFRLSSILLAGTGFASLTLAMALPLWLLVLAAGAFGMAL
                     LRVQSSGAISGAIGHLRFSALTWNIFLLLAFAGFWIDLFLISQEILPAGIHFLVLLLV
                     NKLSNLDQRRDFLHLYAISLIALLASAALTTQIWYAPFFFIYLVIGVWTLLLYHLLQE
                     REELTEPEPGHAHAEPSPVPLPRLTARFFWTTNAMAASAFGLTLLFFFLIPRVGVGFF
                     QNNHGENLRTTGFSEHVDLGVMGPVKQDPSIVMRVELPERTGHDAKWEPLYLRGVAYN
                     RYDGKSWSNSLPHRRMLTELPEGTFTLRTLASKPSPPARQLKLEILLEPLDTTVLFGS
                     PFPTAVKGHFLSLQSDLMGSLYLPFPLHTRSQYTVYSSPTSLNAAEKKSAALIYPDSI
                     LQQYLQVPAVNEQVLELARQVTRPATSVAQAVAMVHTHLLTHYRYNLDVPSLQSPHPL
                     EDFLLTRKTGYCEHYATAMVVMLRSVGIPARLVTGFLATEWNEFGGYYTVRQRDAHAW
                     VEVYFPQSGWITMDPTPPSPNAVGPTWWQSANSAMDSIRLQWDRLFVHYSAHDQFTVV
                     QGIREGGEAVRAGFSETLSALSAQGVAIVSHMVIRLTPSGVPHVVIALVLAAAAVYGG
                     IVILRRFRTRTTPRSPFSTQQQTVIALYTTMLQCCAQQGITKPASATSVEFLEQIRTR
                     WGEAWPLVDSVTRLYARVRFGEAALTEADLATAEVQLRTLRLLKRKTADSQ"
     gene            complement(3033..4175)
                     /locus_tag="PLS35_17490"
     CDS             complement(3033..4175)
                     /locus_tag="PLS35_17490"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNI20519.1"
                     /translation="MAFITLRTWLHQLSRHRAISVTTEGVRFLLLTLAVGIAAVNTGN
                     NLFYLLLAMMLSLIVLSGFLSEQCVRRLEFHRHLPDTLFVNQQATATLWIANRKSHLP
                     SISLRFLDVAAGQDHECGIHLTHLAPGASTLRAYPLLIKRRGRCQLDGIRVVTPFPFG
                     LFHKKAFYPLEASVIVCPALIPLPPMQVQALHAIGHDHALARRGHGNSLYNLREFRPG
                     DDSRAIHWMTTARTAKLMLKETEAEDQRIITLAVFTVAADAEVTTFERALSIAASLID
                     QLLKSGVRLRLLLGDQQEVMAEGNESPLHLFHALALCERRPVDDSTAVQQALISTLVR
                     SQDGPTLLIASWADPALQDLFPGVDQILTPHTHKDLFDADGSGLPA"
     gene            complement(4248..5192)
                     /locus_tag="PLS35_17495"
     CDS             complement(4248..5192)
                     /locus_tag="PLS35_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNI20520.1"
                     /translation="MVNSSQSIKLLQDNIAQVIKGKARAIELAVVCLLARGHLLIEDV
                     PGVGKTTLAHSLARSLDCSFKRIQFTSDLLPSDIVGISVFNRQKQAFEFMPGPLFAHI
                     VLADEINRTTPKTQSSLLEAMSEAQISVDNQTYPLQQPFMVIATQNPAEYHGTFPLPE
                     SQLDRFLMRLCIGYPTPEEEKKVLDRPPSLHPANEIEPVLNAQHILDLQAQVDKVRME
                     DSLMDYLLAIVLATRQNPLFSLGVSTRGALALSKAAKALALARDRTYCLPEDIKELAP
                     AVLSHRVMLNRSQGARTKSVEHTERVIHDILESIPVPV"
BASE COUNT         1117 a         1384 c         1617 g         1076 t
ORIGIN      
        1 tcgccttgcc aaggcgaggg tcgcgggttc aaatcccgtt tcccgctcca atttttcaat
       61 tatctccacg caagatcgtt ccagatctcc ttgtcattga tcgttcctcg tgcacgtgca
      121 ccggtttggg cattacatgc ggcaatgcac gcccctaagt agggacagat agctagccct
      181 ggcgggactc ggcagagtgc aaaccgtata agacatccgt gcggagtacg tacttcagtc
      241 cgctctgcac cacggcacct tcgtgccaaa tggcatgcag aaatacaagc gccattccca
      301 tcttcggccg aaccgacaga tacggagcag gaggttgcag gaatgcatgg tcggcgctcg
      361 catagaactt cgtctccccg cccaccatcc catcattgag atagatcatc atcgtcactt
      421 cacttttgtc tccggtcttc atccgcagat aggagccgtc gcgatgaggc tggaacgtct
      481 ggccgggttg ataccgataa aatcgccacc gttcgttgaa gccgacgaga tgatggccat
      541 caagaacggc tgggaggaag ggttgagcgc gttgaaataa gtttgacgcg agggactcat
      601 catcgacgat cacccgctcg ttattgcgga agttttcatt caccacgcca ccgacggttc
      661 caggttcata ggcggctgct tcactgcgcc ggatcagtga cgcacactct tcctccgaaa
      721 gaaatggtca atgacgaaga ctcgttcggc atcgagcagt tctttaatca caacgacttc
      781 cttccgtttc gcctggcgtg tgaaagtccg gtggtttcac gtagcactag cttgatggat
      841 tgaggcgtga gtgccctggt tactgcgagt cggcggtctt gcgtttgaga agtcggagtg
      901 tgcgcagctg gacttctgcc gtcgcgaggt ctgcctcagt cagcgccgct tctccgaatc
      961 gtactcgagc atacagccgc gtgacggagt caacgagagg ccaagcctcg ccccatcgcg
     1021 tccgaatctg ttcgagaaat tctactgagg ttgcgctggc cggtttcgtg attccctgct
     1081 gcgcgcagca ttgcagcatg gtggtgtaga gcgcgatgac ggtctgttgc tgcgttgaaa
     1141 atggactgcg tggcgtggtc ctcgtccgga acctccgcaa gatcactatc cctccataga
     1201 ctgcggcggc tgccagcaca agagcgatca caacgtgcgg aacacccgag ggagtcaagc
     1261 ggatcaccat atgactcacg atggcgacac cctgcgctga cagggcgctc aacgtctcgg
     1321 aaaatcctgc ccgcacggct tctccgcctt ctcgaatgcc ttgcaccacg gtgaattgat
     1381 catgtgcgct gtagtggacg aacaggcgat cccattggag gcgaatcgaa tccatcgcac
     1441 tgttggccga ttgccaccag gtcggaccga ctgcattcgg cgagggcggg gtgggatcca
     1501 tggtgatcca gcctgattgg gggaaataca cttctaccca ggcatgcgca tctcgttgac
     1561 gtacggtgta gtacccgccg aattcattcc attcagtggc gagaaatccg gtcaccaggc
     1621 gtgcggggat gcctacggag cgaagcatca ccaccatggc cgtcgcatag tgctcgcaat
     1681 agccggtttt tcgagtcagc agaaagtctt cgagcggatg cggagactgc agcgagggga
     1741 catccaggtt gtatcggtaa tgagtcagca aatgcgtgtg caccatggcg acggcttggg
     1801 cgacgctggt tgcaggacgc gtgacctggc gcgcgagttc gaggacctgc tcattcaccg
     1861 cgggcacctg gagatattgt tgcagaatag agtcggggta gatcaacgcc gcggattttt
     1921 tctccgcggc attcaagctg gtcggtgagg aatagacggt gtattggctc cgtgtgtgga
     1981 gggggaacgg cagatacagg gagcccatca gatccgattg gagggagagg aagtggcctt
     2041 tgaccgccgt gggaaacggg ctgccgaaga ggaccgtggt gtcgagtggc tcaagaagga
     2101 tctccagttt gagctggcga gccgggggtg atggtttgga ggctaacgta cggagcgtga
     2161 aggtgccttc gggaagctcc gtgagcatgc ggcggtgcgg caggctgttg ctccaggatt
     2221 ttccatcata ccgattgtag gcgacgcctc gaaggtacag tggctcccac ttcgcgtcgt
     2281 gacctgtgcg ttcagggagt tccactcgca tgacaatgct ggggtcctgc ttgacgggcc
     2341 ccatcactcc caagtcgaca tgctccgaaa accctgtcgt gcgcaggttt tctccatgat
     2401 tgttttgaaa aaaccctact cccactcgcg ggatgagaaa aaagaacagc aacgtgagac
     2461 caaacgcgct cgccgccatg gcgttggtgg tccagaaaaa ccgggccgta aggcggggaa
     2521 ggggcacggg tgacggctcg gcgtgagcgt gccctggttc cggttcggtc agctcttccc
     2581 gttcctgcag caggtggtag agcagaagcg tccacacgcc gatgaccagg tagatgaaga
     2641 aaaacggggc ataccagatt tgcgtcgtca gcgccgcgga ggccaggagt gcgatcaggc
     2701 tgatggcgta gagatgcagg aagtcgcgtc gctgatcgag attcgagagt ttgttgacga
     2761 ggagcaggac aagaaagtga atgcctgccg gaaggatctc ctgtgagatg aggaagaggt
     2821 cgatccagaa tccggcgaac gcaagcagga gaaagatatt ccacgtgagc gctgagaacc
     2881 gcagatgccc gatggcaccg gagatcgcgc ctgaagactg gacgcgcagc aatgccatgc
     2941 cgaaggctcc cgccgccagg actaagagcc acaacggcaa ggccatggcg agtgtcaggc
     3001 tggcgaagcc ggtcccagcc aggagaatgg agctaagccg gaaggcctga tccatcggca
     3061 tcgaagaggt ccttgtgcgt gtgaggggta agaatttgat ccacacctgg aaacagatcc
     3121 tggagggccg ggtctgccca tgatgctatc aagagcgtgg ggccatcctg ggaccgtacg
     3181 agagttgaga tcagcgcctg ctggacggct gtactgtcgt ctacgggcct tcgctcacag
     3241 agtgccaggg catggaacag atgaagcggt gactcgtttc cctcggccat gacttcttgt
     3301 tgatcgccca gcagcaggcg aaggcgaacc ccgctcttca gcaattgatc aatcaaagaa
     3361 gcggcgatag agagcgcccg ttcgaatgtt gtcacttctg cgtccgctgc caccgtaaag
     3421 accgcaagcg tgatgatgcg ttggtcttcg gcttcagtct ctttgagcat gagtttggcc
     3481 gttcgtgcgg tcgtcatcca atggatggct cgcgaatcgt cgcccgggcg aaactcccgc
     3541 agattgtaga gtgaatttcc gtggccacgt cgagccaagg cgtgatcatg gccgatcgcg
     3601 tgtagtgcct ggacctgcat gggtggaagc ggaatgagtg ccgggcagac gatgacgctg
     3661 gcctccagcg gatagaacgc tttcttatga aacagcccga agggaaacgg tgtgacgact
     3721 cggattccgt ccaattgaca gcggccacgc cgtttgatga gcagcgggta cgcacgcaac
     3781 gtcgacgcgc ccggcgcaag gtgagtcaga tggatcccgc actcatgatc ctggccggcg
     3841 gcgacgtcga ggaagcgcaa ggagatgctc ggaagatggg atttgcgatt ggcaatccac
     3901 aacgttgcgg tggcctgctg attgacaaac aacgtgtccg gcagatggcg gtggaactcg
     3961 agtcgccgca cgcattgttc cgacaagaag ccggataaca cgatgaggct gagcatcatc
     4021 gccagcaaca ggtagaacag attgttgccc gtattgacgg cagcgatacc aaccgccaag
     4081 gtaagcagca gaaagcgtac gccttccgtt gtgacgctga tcgcgcggtg tctggagagc
     4141 tggtgaagcc aggtacggag agttatgaaa gccatgctgg tggcccgtca tcccatgagc
     4201 ggccgctggc ggccgttatc cgatagcctg cttcgtgtgc agggtggtca cacgggaacc
     4261 gggatgctct ccagaatgtc gtgaataacc cgttcggtgt gttcgacact cttcgtgcgt
     4321 gcgccctgag atcggttgag cataacgcgg tgcgagagga cggccggagc gagttcttta
     4381 atgtcttccg gcaagcaata ggttcggtct cgcgcaaggg cgagggcctt cgccgctttg
     4441 ctgagcgcga gcgctccgcg cgtactgacg cccagtgaaa ataaggggtt ttgcctggtg
     4501 gcaagcacga tagccagcag ataatccatc aggctgtctt ccattcggac tttatcgacc
     4561 tgggcttgaa gatccagaat atgctgcgcg ttgagaaccg gctcgatttc gttcgccgga
     4621 tgcagcgatg gcggtcggtc cagcaccttc ttttcctctt ctggtgtcgg gtatccgata
     4681 cacagccgca taaggaaccg gtccaattgc gattccggaa gtggaaatgt gccgtgatac
     4741 tccgccggat tttgagtcgc aatgaccatg aacggctgtt gaagaggata ggtctgattg
     4801 tcgacagaaa tctgggcctc gctcatcgct tcgagcaaac tgctttgtgt ttttggcgtg
     4861 gtgcgattga tctcatcggc caggacgatg tgggcgaaga gcggtcccgg catgaactca
     4921 aaggcctgtt tctgacgatt gaagaccgat attccgacga tgtcggaagg cagcaggtca
     4981 ctcgtgaact ggatgcgctt gaaggagcag tccagggaac gggctaaact gtgggccagg
     5041 gtcgttttcc cgacgccggg cacgtcctcg atcagaagat gcccccgtgc caagagacag
     5101 acgaccgcca gttcaatcgc ccgcgccttc cccttgatga cctgcgcgat attgtcctgt
     5161 aacagtttga tagattgaga agaattcacg atgg
//
LOCUS       DAOQEW010000240         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6460_length_5176_cov_7.557313, whole genome shotgun sequence.
ACCESSION   DAOQEW010000240 DAOQEW010000000
VERSION     DAOQEW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6460_length_5176_cov_7.557313"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..887
                     /locus_tag="PLS35_17500"
     CDS             54..887
                     /locus_tag="PLS35_17500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNI20521.1"
                     /translation="MADSSTQSVCWGTVALFTALTILTFVGVPLFAYYYDYTVLDWTL
                     LAILYVVTGMGITVGYHRMITHRSFESHLWVKICLLIMGGWAVQNSALLWCADHIRHH
                     AHTDEEADPYNASKGFWHSHVGWLFINTPYREDRFAAKLRTDPMILWQHRYYWVIVAS
                     GLLLPFAAGYLNGGLMSGLGCFLLAGVLRTVLVLNSTFTINSLCHMVGTQPHGTQDSS
                     RDSWLISFISFGEGYHNYHHTYSHDYRNGSKWYNFDPSKWLIYTLSLVGLTYNLHRER
                     T"
     gene            1160..2056
                     /locus_tag="PLS35_17505"
     CDS             1160..2056
                     /locus_tag="PLS35_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNI20522.1"
                     /translation="MAEEPGGAPHQDEAAARPNIIPGVKHVVAVSSGKGGVGKSTVSV
                     NLAVALALTGAKVGLLDADIYGPNIPMMMGVEKTPEQKDGKIAPAESHGVKLISMGFF
                     VPEDTAVVWRGPMVHTAIQQLFRDVLWGELDYLLIDLPPGTGDAQLTLTQLVPLSGAV
                     TVTTPQEVALHDVRKGMMMFQKVNVPLLGIVENMSFFVCGHCGERTEIFSHGGGERAA
                     EKLGIPFLGRVPIDPVIRAGGDTGNPIVVAKPDSPQAQAFRDIAAKLAAALQTGGVSA
                     TKSRIESLLDKIKRPAADRKPD"
     gene            2084..2389
                     /locus_tag="PLS35_17510"
     CDS             2084..2389
                     /locus_tag="PLS35_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNI20523.1"
                     /translation="MGELIRIAGTADVKPGAGMVAEVNGKAIAVFNVDGTFYAIDNTC
                     VHRGGPLGEGDVVGDVVACPWHNWEFNVKTGACINNPSAKVTAYEVTVEGTDIKVRL"
     gene            2463..2831
                     /locus_tag="PLS35_17515"
     CDS             2463..2831
                     /locus_tag="PLS35_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20524.1"
                     /translation="MADNQLTPKHIEIAETFVRLYVFLTQYIDRCEDEAARKEYPEAE
                     LQKHLGETRAKMVDILKVNPVVKGKVEKECERVLALGSKCLMGGTEKVAALDVLGAER
                     VILKNKTIALSDLLAVYRAL"
     gene            2837..3190
                     /locus_tag="PLS35_17520"
     CDS             2837..3190
                     /locus_tag="PLS35_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20525.1"
                     /translation="MPESAGEKHQLAGFDARERGFNRPVELVRTASGYQATLRYESTQ
                     VVTPDAPTTADALQHMIRILQEQGYTQLRSQQSYRDGVYLGSQELWIEYPDRQPEPEV
                     GLLGLMKKWLGRSPG"
     gene            3318..3761
                     /locus_tag="PLS35_17525"
     CDS             3318..3761
                     /locus_tag="PLS35_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNI20526.1"
                     /translation="MIWLYLLPFTFLVLLHSNPVEAKIYSCEGPNGGTMLTDQPKGKR
                     GCTVVKTPSPSPPGGFTPPVEEAPAAPPDLQPNTFLPSQPVSPIHQPNPRPLDPIDKL
                     QPPTGSQAGGEKAAPEAQRCSPRVNPLNPFAGMNCSPTADETKKP"
     gene            3947..5128
                     /locus_tag="PLS35_17530"
     CDS             3947..5128
                     /locus_tag="PLS35_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20527.1"
                     /translation="MHRDIVCLQIPSFEIVLARTHEASLRHRPVALAPVHTPRGLVRE
                     VSQEALDDGVLPGMSVELARQLCPGLRVIPPDSSRTQAAHRELQQAIMPFAPVWESIQ
                     PGSLFLDLTGTQRLFGRPIDTAARLERELTVKWGWHSQIGVAMNKLVSHLAATTLEKP
                     PQMRSIYSGSEQAFLAPLPATLLPGLNRLQASHILRRLDDLNLRTLGSIADVSLVQLE
                     AVLGTAAVVLHDWAMGIDHSPVRPSVEQPQIEQSLTLNPDEIDDHLILGRLSHLLERI
                     CARLRQQRRMCRRLSLALRHSDHAERMAQHILPYGTYWEADLQPVLAQLFARCFRRRV
                     RIQRIIVRADCLEPLAEQLRLFDESTIAVQPASHRISLVLDAIRAKFGERAIAWGKTL
                     P"
BASE COUNT         1057 a         1598 c         1497 g         1024 t
ORIGIN      
        1 agtatggcgg tgcactgtgg cgatgagttg cgcacaggtt ccgaaggagt acgatggccg
       61 actcatccac acaatccgtc tgctggggca ccgtcgccct cttcactgcg ctcacgatcc
      121 tgaccttcgt cggggttccc ctcttcgctt attactatga ctacaccgtc ctggattgga
      181 cgttgctcgc catactctat gtcgtcaccg gcatggggat cacggtgggc taccatcgga
      241 tgatcactca tcgcagcttt gagagccatc tctgggtcaa gatctgcctg ttgatcatgg
      301 ggggatgggc cgtacagaat tccgctcttc tctggtgcgc tgaccatatc cgtcaccatg
      361 cccatacgga tgaagaagcc gatccgtaca atgcgagcaa aggattctgg cacagccacg
      421 tgggctggct gttcatcaat acaccatatc gtgaagaccg gtttgccgcg aaattacgta
      481 ccgacccgat gattctctgg caacaccggt actactgggt gatcgtcgcc tcaggactgt
      541 tgctgccctt cgcggccggg tatctgaacg gcggactgat gagcgggctc ggctgtttct
      601 tactcgccgg cgtgttgcgc accgtcttgg tgttgaattc caccttcacc atcaactccc
      661 tgtgccacat ggtcggcact caaccgcacg gcacgcagga cagcagccgg gacagctggc
      721 tgatttcgtt catcagcttc ggcgagggct accacaatta tcaccatacc tattcgcacg
      781 actatcggaa cggatcgaaa tggtacaatt tcgatccgtc caaatggttg atctatacgc
      841 tctcgctggt agggctgacg tataacctcc atcgggaacg cacgtaaacg gctggtgaac
      901 aacgcccgcc gctgttgttc tcgcatcgct ccgcccctcg acgtatttct gcgaataccc
      961 cgcggctctt cgctcgctgc gaccgcaccg ggtagagttt tttgaccatc ctctcaactt
     1021 attcctctta catcgctgat ctcttcgacc caaccgcctg ctgcgccgac tgacccattt
     1081 cttttcgccg cggcgatagc ttaatatgcg agaaacccac gtaatccgat cggaccaacg
     1141 gggtggagga ggcatcacta tggcagaaga accaggcggc gcaccgcatc aagacgaagc
     1201 ggccgcccga cccaatatta ttcccggagt caaacatgtc gtcgccgtca gcagcggcaa
     1261 gggcggcgtc ggtaaatcca cggtctccgt caatctcgcg gtcgctcttg cgctcaccgg
     1321 cgccaaggtc ggattgctgg atgccgatat ctacgggccc aacatcccga tgatgatggg
     1381 ggtcgagaag actcctgagc agaaagacgg caagattgcg ccggcggaaa gccacggcgt
     1441 caagctgatc tcgatgggat ttttcgtgcc ggaagacacg gccgtcgtgt ggcgaggccc
     1501 gatggtgcac acggccattc agcaactctt ccgcgatgtg ttatggggcg agttggatta
     1561 tttgttgatc gacctgcccc cgggaaccgg agacgcgcaa ctcaccctga cgcagctcgt
     1621 gccgctgtcg ggcgccgtca cggtgaccac gccgcaggaa gtggcgttgc acgacgtgcg
     1681 caaaggcatg atgatgtttc agaaggtgaa cgtgccgctc ctcggcatcg tggagaacat
     1741 gagctttttc gtctgcggcc attgcggaga gcggacggag attttctccc atggcggcgg
     1801 agagcgggcg gcggagaagc tgggaattcc ctttcttgga cgggttccca tcgatcccgt
     1861 cattcgcgcc ggaggcgata ccggcaaccc gatcgtggtc gccaagcccg attccccgca
     1921 agcgcaggcg tttcgcgaca tcgcagccaa actggccgca gccttgcaaa cagggggcgt
     1981 gtcggcgacc aagagtcgca tcgaaagttt attggataaa atcaaacggc cggctgcgga
     2041 tcgaaagccg gactaggacg agtgacgtac tcagggagga tgtatgggcg agttgatcag
     2101 aatcgcagga accgccgacg tgaagccggg agccggcatg gtcgcggagg tgaacggaaa
     2161 agccattgcg gtcttcaatg tggacgggac cttttatgct atcgataaca cctgcgtgca
     2221 tcgcggcggc ccgctgggcg aaggcgatgt ggtaggggat gtcgtcgcct gcccatggca
     2281 taactgggag ttcaacgtga agacgggcgc ctgcatcaat aacccgtcgg ccaaggtgac
     2341 ggcctatgaa gtcacggtcg aaggcaccga tatcaaagtc cggctgtgat gcagggacgc
     2401 gccgcgcgcg acgtcgccac agcataatga tcatgtcgac accttagtga atggaggatc
     2461 caatggcgga caatcagctg accccgaaac acatcgagat cgccgaaacc ttcgtgcggc
     2521 tctatgtctt tctgacccag tacatcgacc gctgtgaaga tgaagccgcg cggaaggaat
     2581 accctgaagc ggaactccaa aaacacctgg gggagactcg cgccaagatg gtggacatcc
     2641 tgaaggtgaa tcccgtcgtg aagggcaagg ttgaaaagga atgtgaacgc gtccttgcgt
     2701 tagggagcaa atgtctgatg gggggaacgg aaaaggtcgc cgcattggat gtgttgggtg
     2761 cggaacgcgt gatcctcaag aataagacca ttgcactcag cgatctgctg gccgtctatc
     2821 gcgccttgta agaccaatgc cggaatcggc tggagagaaa caccaactcg ccgggttcga
     2881 cgccagggag cgagggttca atcgccccgt ggagttggtg cggacggcct ccggctatca
     2941 ggcgacgttg cggtacgaat ccacgcaggt ggtcacgccc gacgcgccga ccacggctga
     3001 cgcgctccag cacatgatcc ggatattgca ggagcagggt tacacgcaat tgcgcagcca
     3061 gcagagctat cgcgatggcg tttatctcgg ctcgcaggaa ctctggatcg aatatcccga
     3121 tcggcaaccg gagccggagg tcggcttgct gggcctgatg aagaaatggc tgggacgttc
     3181 acccggctga tgtcccccta agcccgctcc cgcagattga caggttccgg cctcgcgcct
     3241 atagtcgtgc gggatcatgg cgctccgcgg gaacgccatc cctcatccgc ctctgatccc
     3301 attcggggaa atcgacgatg atctggctgt acctcctgcc cttcaccttc ctcgttctcc
     3361 ttcactcgaa cccggttgaa gcaaagattt acagctgcga gggcccgaac ggcggcacca
     3421 tgctgacgga ccagccgaaa ggcaaacgcg gctgtacggt cgtcaagacg ccatcaccgt
     3481 ccccgccagg cgggttcacg ccaccggttg aagaagcgcc ggcagctccg ccggacctgc
     3541 agcccaacac gtttctccct tctcagccgg tctcgccgat tcatcaaccc aatccgcgcc
     3601 cgctcgatcc gatcgataag ctccaaccgc caaccggctc acaggcggga ggggagaagg
     3661 ccgcaccgga agcgcaacgc tgctcgccgc gcgtgaaccc gctgaatccc ttcgccggca
     3721 tgaattgttc acccacggcc gacgaaacga aaaaaccgta ggccccttca attggcagaa
     3781 taacctccgc cagaacgatt ctctttcacg tcccctctca gaagcttccc cacaccacgt
     3841 tcacgttcgt cgcgcttctg cgttcgagac gctgatgggt cgtccgcacc attgacaccg
     3901 cccccttccc gatgtaacca ttggttacac tcacaaagat accatcatgc atcgcgacat
     3961 cgtctgtctc cagattccgt cgttcgagat cgtgctcgcg cggactcacg aggcgtcgtt
     4021 gcgtcaccgg ccggtcgccc tggcgccggt ccatacgcca cgcggcctgg tccgtgaagt
     4081 ctcacaggaa gcccttgacg atggggtcct gccgggcatg tcggtcgaac tcgcccggca
     4141 attatgtccg ggccttcgtg tgatcccgcc ggactcgtcg agaacgcagg cggcgcatcg
     4201 tgaattgcaa caagcgatca tgccctttgc gcctgtgtgg gaatcaatcc aacccggttc
     4261 gctatttctg gacctcaccg gcacacaacg gctcttcgga cgtcccatcg atacggccgc
     4321 ccgcctggaa cgggaactga cggtcaaatg gggatggcac agccaaatcg gggtggccat
     4381 gaataagctg gtgtcgcacc tggcggccac gacgctggag aagccgccgc agatgcgttc
     4441 gatctattcc ggctccgagc aagcctttct cgcgccgttg cctgccacgc tgctgccggg
     4501 actcaacaga ctgcaggcgt cgcacatcct tcgccgcttg gacgatctga atctgagaac
     4561 cttgggatcc attgccgacg tgtcattggt ccagttggaa gcggtgctcg gaaccgcggc
     4621 cgtcgtgctg catgactggg ccatgggtat tgaccactcg cccgtccggc catcggtcga
     4681 acaaccgcag atcgaacaat ctctcaccct caatccggac gaaatcgacg atcacctcat
     4741 tctgggacga ctgtcccatc tgctcgaacg gatctgcgcc aggctccggc aacaacggcg
     4801 tatgtgcagg cgcctgtcgc tggcgctccg ccacagcgac catgccgaac gcatggctca
     4861 acacatcttg ccgtacggca cctattggga ggcggatctt cagcccgtgc tcgcgcagct
     4921 cttcgcccga tgtttccggc gtcgggtcag gatccagcga atcatcgtgc gggccgattg
     4981 tctcgaaccg cttgccgagc aactgcggct gttcgacgag tcgaccatag cggtccagcc
     5041 ggcgtcccat cgaatctccc ttgtccttga tgccattcga gcgaaattcg gcgaacgggc
     5101 gatcgcgtgg ggaaagacgt tgccatgacc accacgccct tcgtgcacct gcacgtccat
     5161 tcggattatt caccca
//
LOCUS       DAOQEW010000241         5125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6564_length_5125_cov_7.376726, whole genome shotgun sequence.
ACCESSION   DAOQEW010000241 DAOQEW010000000
VERSION     DAOQEW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6564_length_5125_cov_7.376726"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..167
                     /locus_tag="PLS35_17535"
     tRNA            92..167
                     /locus_tag="PLS35_17535"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Val,seq:tac)
     gene            301..378
                     /locus_tag="PLS35_17540"
     tRNA            301..378
                     /locus_tag="PLS35_17540"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:336..338,aa:Asp,seq:gtc)
     gene            451..1167
                     /locus_tag="PLS35_17545"
     CDS             451..1167
                     /locus_tag="PLS35_17545"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNI20528.1"
                     /translation="MFQSGVMGLVGSLGAVSKIVLLLLLVASILSWGVILYKWRTFKA
                     ADREDQRFFNAFTKIRDLDELYRQSKRAEGSPSARVFQGIMDRVVTASGDGAVSPSLA
                     AAGSKESAAAFDHQYMDKTVSYLVQNQVSHLEAYLPVLATTGNITPFIGLLGTVLGII
                     DSFREIGMQGTASIAAVAPGVSEALVATAAGLFTAIPAVIFYNYYLTRIRKTVFRIES
                     FTVEAMRSLQTRLKQPQAGV"
     gene            1170..1622
                     /locus_tag="PLS35_17550"
     CDS             1170..1622
                     /locus_tag="PLS35_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNI20529.1"
                     /translation="MTSETRHRRFMAEINVIPLVDVVLVLLVIFMVTAPMLYRGMDIN
                     LPKSASNTIKPEARAVLSIERDQRLYLDKDAVSVVQLERKLRALKDQSPDVSLYLRAD
                     RDVPYGIVVQVMDSVKKAGIEKLGMVTEPTGAERVNESVATPSQPRKK"
     gene            1715..2905
                     /locus_tag="PLS35_17555"
     CDS             1715..2905
                     /locus_tag="PLS35_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNI20530.1"
                     /translation="MGEAGGDRFRKTVVVSLLLHLCLLSIIMGAKFFKKTERPLSAVE
                     VSLVTLPPVEAKPEPQVEKVEKAVPKPAPKPVQSAPIPVPPKPVQAPTPTPPPVQSAP
                     VAKPAPPAPAPAPAPVPVPKLEAPVLTAPQPIPQAAKSPSSAPVTNRADVLRDVMKDV
                     ELPLNAPKYGDIAPMKPAEVKKSAQAKLGAPERSDLDAMLKKLNVPDMAAPQVAVPQE
                     PPKPNVTPAKRASLSEEMTSDLDRELQDLKKLQAPPPVKATEPVREVKPMFREPQPPV
                     TAPPVAASVTRSKPTPMLRVPGMSGSNPYLARVQARISGFWTAPPVDISGKALTVTVR
                     FRLERDGRVGSVIIEQSSGNDYYDMAAQRAVQSAIPLPPFPPDLTDSYFDAHFTFAVG
                     EAAG"
     gene            2902..4233
                     /gene="tolB"
                     /locus_tag="PLS35_17560"
     CDS             2902..4233
                     /gene="tolB"
                     /locus_tag="PLS35_17560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNI20531.1"
                     /translation="MNRVIIGLMLTLCVVVGAGLFGILDSRATDVFLEATRPDFQKIP
                     IGVFGFQNGGGPEWLGGRLEEVLKADLQRSLVFSLVDLPGIGVKAREVSTTDKAVFKQ
                     AAENGVSVLVWGKSGQKNGSKDSELLMDGFVYDSGSDEVVGGKRYVGSTSVVRLMAHR
                     FADELVFRYTGEPGIARTKIVYVAEHGNARELFVMDYDGYEPKQITADGFLNLMPRWS
                     PDRRFIVFTAYRSRNTQDIDILELATGKRWTLVSMPGLNITPALSPDGNYLAFASSQD
                     GNSEIYKLDTRTKTPQRLTVNQGGDLSPTWSPTGREIAFTSDRGGAPQVFVMSADGSN
                     VRRLTYEGDYNAAPAWSPRGNWIAYVCRTAQRLYKLCIVSPDGQKRVQVTTGPGIDDS
                     PSWSPDGRHLTFSSTVDGKSHIYMVDTDGKNLERITFGGTHNSSPSWSPAL"
     gene            4301..5050
                     /locus_tag="PLS35_17565"
     CDS             4301..5050
                     /locus_tag="PLS35_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI20532.1"
                     /translation="MRIRVATMGLTMVVGMLLVMQGGCSKKSIQSGGDAQSSDRGMAK
                     SGAPAQSQAQLPPPTGLDAPSATFPDLSLSSKPEDPETGGLRGFDSVSGGKAPSEERL
                     GGGGTMLAKVEPSESTARQIEEIRREQAKEQAASAEAGLRDVFFGYDSWTITEDGRQS
                     LTQDAQWIKANAGALVKIEGHCDERGTLAYNLVLGEKRAKAVRNYLVELGVGANRLSV
                     VSYGKERPFCNERSESCYQQNRRGHVVVRSK"
BASE COUNT         1050 a         1384 c         1611 g         1080 t
ORIGIN      
        1 aaaatcttgc aagaataatg catgattctt ggttgacact gcttggtgga gttgatagca
       61 tcccaaaaat ctgccgggat cgaggaaaaa cgggcggata gctcaattgg gagagcgctg
      121 cccttacaag gcagaggtca caggttcgat ccctgttccg cctaccaata agaaatacaa
      181 gaaattgtgc gcggggcatt gccttatcca ttagtagtga tgtacaagag tacgcgctac
      241 tgtatttaga cagtttgatt ctgaagatct gaattcttct tccgccatct tgggttgcgt
      301 ggggccgtcg ttcagcctgg ttaggacgcc agattgtcaa tctggaagtc gcgggttcaa
      361 atcccgtcgg ccccgccatt cccccagctt ttggtacaat ccatgctacc gttttcatgc
      421 gacgccatgt gaagggaaag gtctaggctc atgttccagt caggcgtaat ggggttggtg
      481 ggctcgctgg gagcggtatc gaaaatcgtg ctcctgctcc tcttagtcgc ctcgattctt
      541 tcctggggcg tgattttgta caaatggcgg acgttcaagg cggctgatcg tgaagaccag
      601 cggttcttca atgcctttac gaagatccgt gatctggatg agttgtatcg gcagtccaag
      661 cgcgcagagg gcagccccag tgcgcgcgtg ttccagggaa ttatggatcg cgtggtgacg
      721 gcctcgggag acggggcggt gagtccgtcg ctggccgcgg ctggatcgaa agaatcggcc
      781 gccgccttcg atcaccagta catggataaa acggtgtcgt acctcgtgca gaaccaggtc
      841 tctcatttgg aggcctacct gccggtgctg gcgaccacgg gcaacattac gccgttcatc
      901 ggcttgctcg ggacagtgct gggtatcatc gactcgtttc gtgagatcgg catgcagggc
      961 acggccagta ttgccgccgt ggctccgggc gtatctgaag ccctggtggc gacggccgcc
     1021 ggcctgttca ccgccatccc tgcagtcatt ttctacaact attatctgac gcgtattcgg
     1081 aagaccgtgt ttcgcatcga gtcattcacc gtcgaagcga tgcgttcatt gcagacgcgc
     1141 ttgaagcagc cccaggccgg agtgtagtga tgacgtcgga aacccgccat cgccggttca
     1201 tggccgagat caatgtgatt ccgctggtcg acgtggtgtt ggtgttgctc gtcattttca
     1261 tggtgacggc gccgatgctg tatcgcggca tggacatcaa tcttcccaag tccgcgagca
     1321 atacgattaa gcctgaagca agagcggttt tgtccattga gcgcgaccag cgattgtatt
     1381 tggataagga cgcggtgagt gtcgtgcagt tggagcgaaa actccgggcg ctcaaagacc
     1441 agagcccgga tgtgtcgctg tatctgcgtg cagaccggga tgtgccgtat gggatcgttg
     1501 tgcaggtcat ggatagtgtc aagaaagccg gcattgaaaa gcttggtatg gtgacggagc
     1561 ccacaggcgc cgagcgggtc aatgagtcgg ttgcgactcc ctcgcagcca cgcaagaagt
     1621 agcggcgccg cgactcgcgc gccatacggt cgtatcgcaa gtcatgacgt tccaagccct
     1681 gccaagacat accgcgctgt ccctaatcgg agatatgggt gaggccggag gtgaccgttt
     1741 tcgaaagacg gtcgtcgtgt cgttacttct gcacctgtgt ctcctctcca tcattatggg
     1801 ggccaagttc tttaaaaaaa cagaacgtcc gctttcggcg gtggaagtct ctctcgtgac
     1861 gttgccgcct gtggaagcga agcctgaacc gcaagtggag aaggtggaga aggcggtacc
     1921 gaagcccgca cccaagccgg tgcaatcggc tccgattccc gtgcctccta aaccggtgca
     1981 ggccccgacg ccgactccgc cacctgttca atcggcgccc gttgcgaagc cggcaccacc
     2041 ggcgccagca cctgcgcctg ctccggttcc cgttcccaag ctcgaggctc cggtattgac
     2101 ggcacctcaa ccgattccgc aggcggccaa gtcaccttct tctgcccccg tcacgaatcg
     2161 ggcggatgtg ttgcgtgatg tcatgaaaga tgtggagctc ccgctcaatg ctccgaaata
     2221 tggagacatt gcgccgatga agccggcgga ggtcaagaaa tctgcgcagg cgaagctcgg
     2281 agcgccggaa cgctctgatc tggacgccat gttgaagaag ctgaacgtgc ctgatatggc
     2341 tgcgcctcag gtggcggttc cacaagagcc cccaaagccg aacgtcacgc cggcaaaacg
     2401 cgcatcgctg tcggaggaaa tgacgagcga tctcgatcgt gagcttcagg atttgaagaa
     2461 actgcaggcg cctccgcccg tgaaagcaac ggagccggtg cgagaagtga agccgatgtt
     2521 ccgcgaaccg caaccgccgg tgaccgcgcc gccggtggcg gccagtgtca cacgcagcaa
     2581 gcccactccc atgctgcggg tgccgggcat gtcagggtcg aatccgtatt tggcgagggt
     2641 tcaggcacgg atcagcggat tctggacggc gccgccggtc gatatttcag gcaaggcttt
     2701 gacggtgacg gtgcggtttc ggttagagcg ggatggtcgg gtcgggtcgg tcattatcga
     2761 gcaatcttcg gggaacgact attacgacat ggctgcacaa cgcgcggtgc aaagcgcgat
     2821 tccgttgccg ccctttccgc ccgacctgac ggattcgtat ttcgatgctc acttcacttt
     2881 tgccgtaggc gaggctgcag gatgaatcgt gtgatcatcg gtcttatgtt gaccctctgt
     2941 gtcgtggtgg gcgccggact gttcggaatt ctggactccc gcgccaccga tgtctttctt
     3001 gaggcgaccc ggcctgattt ccaaaagatc cccattggtg tgttcgggtt tcagaacggt
     3061 ggcggcccgg aatggctcgg ggggcgactc gaagaagtgt tgaaggccga ccttcaacga
     3121 tctcttgtgt tctccctcgt ggatctgccc ggaatcggtg tgaaggcccg cgaggtgtcg
     3181 accaccgata aggccgtgtt caaacaagcg gctgagaatg gggtctccgt cctggtatgg
     3241 gggaaatccg gccagaagaa cggcagtaag gacagcgaac tgctgatgga tggcttcgtg
     3301 tatgacagcg gcagcgacga ggtcgtcgga ggcaagcgat acgtcgggtc aacatcggtc
     3361 gtccggttga tggcccatcg gtttgccgac gagttggtgt ttcgttatac cggcgagcca
     3421 gggatcgcca gaaccaagat tgtctatgtc gcggagcatg ggaatgcccg tgaattgttt
     3481 gtgatggact acgacgggta tgagccgaag cagatcactg ccgacggctt tctgaatctc
     3541 atgccccgat ggtcgccgga tcgccggttt attgtcttca cggcctatcg gagtcggaac
     3601 acgcaggaca tcgatattct ggagctcgcg acggggaagc gctggacgct ggtgtcgatg
     3661 cccgggctga acatcacccc ggccttgtcg ccggacggaa attatttggc ctttgccagc
     3721 agccaggacg gcaattcgga gatttataag ctcgacacgc gaaccaagac tccgcagcgc
     3781 ctgaccgtga atcagggagg cgatttgtcg cccacctggt ctccgaccgg acgggaaatc
     3841 gcgtttacct ccgatcgcgg cggcgccccg caagtgtttg tcatgagcgc cgatggctcc
     3901 aacgtccgcc gcttgacgta cgagggggat tacaatgccg caccggcctg gtcgccgcgc
     3961 ggcaattgga tcgcctatgt ctgtcgaacc gcccagcggc tgtacaaact gtgcatcgtg
     4021 tcccctgatg gtcaaaaacg ggtgcaggtt acgacgggcc cggggatcga cgactcgcca
     4081 tcctggtctc ctgatggccg ccacctgacc ttcagttcaa ccgttgacgg aaagagccac
     4141 atctacatgg tggatacgga cggaaaaaat ctggaacgca tcacattcgg cggtacacac
     4201 aatagttcac cgtcatggtc tccggcactt taggattgag tacgcaattg acaggatcta
     4261 caggcatact aaccattcac cagcgatgag gagtgacgat atgaggatac gggtagcgac
     4321 aatgggcctg accatggtag tcgggatgct gttggtcatg cagggcgggt gttccaagaa
     4381 atcgattcaa tccggtggcg atgcgcagtc ctctgatcgc gggatggcga agtcgggcgc
     4441 tccggcgcag tctcaagctc aactcccgcc tccgacaggg ttggatgctc ccagcgccac
     4501 cttcccggac ctgtcccttt ccagcaaacc ggaagatcct gagacgggcg gcttgcgcgg
     4561 gttcgattca gtgtcgggcg gcaaggctcc gtctgaggag cggcttggag gcggcggcac
     4621 gatgttggcc aaggtcgagc cgtccgaaag cacggcacgc cagattgaag aaattcgtcg
     4681 ggagcaggcg aaggagcagg cggcgtcggc ggaagccgga ttgcgcgacg tgtttttcgg
     4741 ctacgatagc tggacgatta cggaagatgg ccggcagtcg ttgacgcagg atgctcagtg
     4801 gatcaaggcg aatgccgggg cgttggtcaa gatcgaaggg cattgcgatg aacggggcac
     4861 gctggcctac aatctcgtgt tgggcgaaaa gcgagccaag gccgtgcgaa actatctggt
     4921 cgaactgggc gtcggcgcca accggttgtc ggtggtgtcg tatggcaaag agcggccatt
     4981 ctgcaacgag cggagcgaaa gctgctatca gcagaaccgt cgtgggcatg tggtggtccg
     5041 ttcgaaataa ctgttccaat catggatgat gtgacatgat tctccggaac agacgccggt
     5101 caagggagac ctgtcatgga cagtg
//
LOCUS       DAOQEW010000242         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6652_length_5083_cov_6.409109, whole genome shotgun sequence.
ACCESSION   DAOQEW010000242 DAOQEW010000000
VERSION     DAOQEW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6652_length_5083_cov_6.409109"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLS35_17570"
     CDS             complement(<1..345)
                     /locus_tag="PLS35_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20533.1"
                     /translation="MTLRAFGATVVKPMATRSLDRILTLEYHDTSPSGKNQMGLLVVD
                     GRIRRYPTKRFMRWLTNVEREFRTQLKGMQPYLPIQGECYAIVEYVPGDLITRDLPGM
                     QDAVWHMCERFGI"
     gene            complement(403..2190)
                     /locus_tag="PLS35_17575"
     CDS             complement(403..2190)
                     /locus_tag="PLS35_17575"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI20534.1"
                     /translation="MVCSGSLEVRVQGEVVSAEMAGSPEEMPKVVGVITSRTRARRGS
                     GDWIEIARKHRAFIPDGLSAPSVGEVGECWGRVIANGPDPFLMVSRIRSRRFEACASS
                     SELLGVLGLRRSPPRQVVKVFQLLGPLVEWLCCAGWKAVARKLVKGTLRSVQAIAADP
                     FLLYRRRGLPFQAAVAAAQVLGHDLYSDEHYKAAVMEALAQADEEGIGGISDAHLVAR
                     CAELIGLPADRVASSITLQAGRAGKCEQGLWFSPGVYFVRKKALAMIRANQQDSGNAS
                     PSVQARALRYRYTVVTGGAGSGKTELARSLSAQVRERGGTVAATAMTGKAATLLGEDA
                     TTLHKLLGYGGGGYSVSSVGADLVLVDEAGMLTWHILYRLLLSCRGQIVLIGDPQQLA
                     PVGATPVMAELLTVLPVVHLGEEGFKGSLLVKVQVIRFASEALLLHQLRKVVCGYQDT
                     GVEWQALSPVYAGGLGVDRLNRLLQEIVNPDGLPCHGGFRTGDRVIVTKTRYDGGQRA
                     VNGEQGRVLGGMGDTIALRLDSGREVALRADELQLSYCITVHKAQGSRYQCVVFIIPE
                     RECGAFAVEERMQYVGRTRGREETVCMVY"
     gene            2350..3486
                     /locus_tag="PLS35_17580"
     CDS             2350..3486
                     /locus_tag="PLS35_17580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNI20535.1"
                     /translation="MLAELIHPLALMEHQEWEYRPRPSNAGPDHCIRKLTYHARHAPA
                     KSPSGRFLVVLDDSTWHEELVLNWIAKSTFHLHSQQLELTCATTTFQGQPFPIKGHID
                     GVITDLLGIDRLLELKAVEHFTFTRYAEGNYPTNYLVQVVFYLVGLAALNPHIDEALL
                     LIKNKNQSAFLEYRVRYDRENDRLTVLEITHSTGTRVYPHQDFVGLYQEALRRFEEVE
                     QYRDQDALPPRPYPNADNFHCAYCPYQFCWEGVDQVPIEGIAELRTGLVPAVEEYLDL
                     QTELAALTPKQKRLDALKQQFKLELRTEGITRAEGHGYYIASSPYEQSKLDQTKLSKA
                     TIERATVKETYSRFSAGRLSKVVDATKPKRRSPLKKPKPTTQIA"
     gene            3525..4859
                     /locus_tag="PLS35_17585"
     CDS             3525..4859
                     /locus_tag="PLS35_17585"
                     /inference="COORDINATES: protein motif:HMM:NF039495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20536.1"
                     /translation="MSDADKPTFIPMNRINGISNRRRMPIIGKIRLGVKVSGPSGFYP
                     KETPHFVVPEEVAKVFGAQPKELEVMFVSNDPTVNCPQALRWYGESAGLQCIGNNQVA
                     RRLNQDTWKTESRSCPCEKLESGECGPRANLIFMIPKVSSGGLFQIDSSSINSMEKIN
                     SYFDFLALTLGRIAMIPLKLRRVPVTRRREGRPTTHYPLELRYEGSADETTRHQQETE
                     TVLRKIKMLEIQEPIEINPVLDPGAHVVMEEELEEDPSPIPAESVGEPGPETHNPAIP
                     PMSTPETAAAKAPEATHPPAGPPISLAQTNYLLRLTKDLPLTEDEVRVKVRALTQREA
                     SGLIKKLLAEDFSFFTSSPNTPPATPVNTGSAADTMTDPQFKKIMDLVLSMGIPEPHV
                     KTLTAGYTKRQASTLIDQLVSGDFSAFQEMKEEELPILTTSGTVPGEEEPEF"
BASE COUNT         1227 a         1680 c         1195 g          981 t
ORIGIN      
        1 aatcccaaat cgttcgcaca tatgccagac cgcatcctgc atccccggga gatcccgtgt
       61 gataagatcg cccggcacat actccacaat cgcataacac tccccctgga tcggtaggta
      121 cggctgcatc ccctttagct gagtcctaaa ctcccgttcc acattcgtca gccaccgcat
      181 gaagcgcttc gttggatacc ggcgaatccg gccatcgaca accaacagcc ccatctgatt
      241 cttccccgac ggactcgtat cgtgatattc caatgtcaga atacggtcca ggctccgtgt
      301 cgccatgggc tttaccactg tggctccgaa agcacgcaag gtcatgggcc tccgttgctg
      361 tgaacctcgc cattgaccgg gcctcatgca ccagccccca cgctaataca ccatacagac
      421 tgtctcctca cgtccacgtg ttctgccaac atattgcatg cgctcctcga ctgcaaaggc
      481 cccacattcc cgctctggaa tgataaacac cacgcactgg tagcgacttc cctgcgcttt
      541 atgcacggta atgcaatagc ttaactgcaa ctcatccgcc ctgagtgcta cctcgcgccc
      601 agagtccagg cgcaacgcta tcgtgtcacc cattccccca agcacccgcc cttgctcacc
      661 attgactgct cgctgaccgc catcgtatcg agtcttcgtg acaatgacgc gatcgcccgt
      721 tcgaaaccca ccgtgacatg gcagtccgtc cggattcaca atctcctgca gcaatcggtt
      781 caaccgatcc acaccaagac ctcctgcgta cacaggggac aacgcctgcc actcgacccc
      841 agtgtcttga tagccacaca ccactttccg caactgatga agcaaaagcg cctcggatgc
      901 gaagcgaatc acctggacct tgaccagcaa cgaccccttg aatccttctt cgcccaagtg
      961 caccacaggc aagacagtca acagctccgc catcacagga gtcgctccca ccggggctag
     1021 ttgctgcgga tcgccgatca acacgatttg cccgcgacag ctcaacaaca atcgatacag
     1081 gatatgccag gtcagcatgc cagcctcatc gaccaagacc aagtcggcac ccacactcga
     1141 caccgaatag ccgccgcctc cataccccag caacttgtgc aacgtcgtgg cgtcttcccc
     1201 gaggagggtc gccgctttcc cggtcatcgc cgtggccgcc accgtccctc ctcgctcacg
     1261 cacctgagca ctgagagacc tcgctagctc agtctttcca cttccagccc caccggtaac
     1321 caccgtatac cgatagcgca acgccctggc ttgcacgctg ggactcgcgt tacctgagtc
     1381 ctgctgattc gctcgaatca tggccagggc tttcttccgc acaaaataca cccctgggga
     1441 aaaccacaag ccctgctcgc atttccccgc acgtcctgcc tgcagtgtga ttgacgacgc
     1501 tacccgatcc gcaggcagtc caatcagctc tgcgcaccgt gccacaagat gagcgtcaga
     1561 aatccctccg atcccttctt catcggcttg tgcaagggcc tccatcactg cggccttata
     1621 gtgttcgtca ctatacaagt cgtgccccaa cacctgtgcg gccgccaccg cagcctgaaa
     1681 tgggaggcct cgacgtcgat atagtagaaa cggatctgca gcgatggcct gcaccgatcg
     1741 tagcgttccc tttacgagtt tgcgcgccac ggctttccag cctgcacaac acagccattc
     1801 caccagcggg cccaacaatt gaaacacctt cacgacctga cgtgggggcg aacgacgcaa
     1861 gccgagcacc cccaacaact ccgatgagct cgcgcaggct tcgaatcgtc gtgatcgtat
     1921 cctggacacc atcagaaagg gatcgggccc attggctatc actcgccccc agcactctcc
     1981 cacctctccg accgaagggg cggaaagtcc gtccggaata aacgcccgat gcttacgagc
     2041 tatttcgatc caatcgccag acccacggcg cgcacgggtc cgactcgtga tcaccccaac
     2101 caccttaggc atttcctcag gactgcccgc catctcggct gagacgacct ccccttgcac
     2161 gcgcacttct aaggatccag agcagaccat gcttctccct ccccatgctt cagccatcac
     2221 gcactcatct ctgcaactct gtgacatacc gttttggaac caaaacggta tgtcacagct
     2281 cgttcacttc agcgaaccgc acgacctccc ggtcaatcga cgcgctgcac gaactcaccc
     2341 ggaggcccta tgctcgctga actcattcat ccactcgctc tcatggaaca tcaggaatgg
     2401 gagtaccggc ctcgtccctc gaatgccggt ccagaccact gcatccgcaa actcacgtac
     2461 catgcccggc atgctcccgc aaaaagtcca agcggtcgat tcctcgttgt cctcgacgac
     2521 agtacttggc acgaagaact ggtgttaaac tggattgcta agagcacgtt tcatctccac
     2581 agccagcagc ttgaactgac ctgcgcgacc acgaccttcc agggtcagcc cttcccaatt
     2641 aaaggccaca ttgacggcgt catcacggac ctcttgggaa ttgatcgcct tctagagtta
     2701 aaggctgtcg aacacttcac cttcactcgc tatgcagaag ggaattaccc gactaactat
     2761 ctcgtacaag tggtcttcta tctggttggt ttggccgcac tgaatcctca tatcgacgaa
     2821 gctctcctcc tcatcaaaaa caagaaccaa agcgcattcc ttgaatatcg agtccgctac
     2881 gaccgggaaa acgaccgcct gacggtcctg gaaatcaccc acagtacagg cacacgcgta
     2941 tacccccacc aagacttcgt gggcttgtat caagaggcac tccgacgctt tgaagaggtg
     3001 gaacagtacc gtgatcagga cgcgttgccc ccacgcccgt accccaatgc cgacaacttc
     3061 cattgcgcct actgtccgta tcagttttgc tgggaagggg tcgaccaggt ccccattgag
     3121 ggaatcgcgg aacttcgtac tgggctggtg ccggccgtgg aggagtatct cgacctacaa
     3181 actgagcttg cagcgctcac tccgaaacaa aagcgactgg acgccctaaa gcagcaattt
     3241 aagttggaat tgcgcaccga ggggatcacg cgcgcggagg ggcatggcta ctacatcgcg
     3301 tcatcaccct atgaacagag caaattggat caaaccaaac tctctaaagc cactattgaa
     3361 cgcgcgaccg tgaaggaaac ttacagccgt ttctcagcag gccgactgtc caaagtcgtc
     3421 gacgcaacca aaccgaaacg ccggtcccct ctcaaaaagc ctaaaccgac gactcaaatc
     3481 gcgtaactgt attcatcagc tgattatcat gactaggagg catcatgtcc gacgcagaca
     3541 aacccacgtt cattcccatg aaccgcatta acggcatttc taaccgacgc cgcatgccta
     3601 tcatcggcaa gatccgtctc ggcgtaaaag tgagcggccc cagcgggttc taccccaagg
     3661 aaacgcctca tttcgtggtc ccagaagaag tagccaaggt cttcggggcg caacccaagg
     3721 agttggaggt gatgttcgtc tccaacgatc ccacggtcaa ctgcccgcaa gctctccgct
     3781 ggtatggaga atccgccgga ctacaatgca ttgggaacaa tcaggtggct cgtcgcctga
     3841 atcaagacac ctggaaaact gaatcgcgat cttgtccatg cgagaaactc gagtccggag
     3901 aatgcggacc acgcgcaaat ctcatcttca tgatcccgaa agtcagcagc ggcggcctgt
     3961 ttcagatcga ttccagcagt atcaactcga tggagaagat taactcctat ttcgatttcc
     4021 tcgcgctcac cctcggacgt attgccatga tcccgctgaa actccgtcgc gtgcccgtca
     4081 ctagacgccg cgaaggacgc ccgaccactc attatccgct cgaattgcgc tatgaaggct
     4141 cagcggacga gacgaccagg caccaacagg aaacggagac ggtcctgaga aagatcaaga
     4201 tgttggaaat tcaggagccg attgaaatca acccggttct ggatccaggc gcacacgtcg
     4261 tcatggaaga ggagttagaa gaagatccca gccccatacc agcagagtcc gtgggagagc
     4321 ccgggcctga gacgcacaat ccggcgatcc caccgatgtc cacgcccgag acagccgccg
     4381 cgaaagcgcc ggaggccact cacccaccag caggtccgcc catctcactg gcccagacca
     4441 actacctgct tcggctaacc aaggacttgc cactcacaga ggatgaggtc cgcgtgaaag
     4501 tccgcgcact cacacagcgc gaagcgtcgg gactcatcaa gaaactcctc gctgaggact
     4561 tttctttctt cacatcgagc ccgaacactc ctccggccac gcctgtgaac acagggtcag
     4621 cagccgatac catgaccgat cctcagttta agaaaatcat ggacctcgtc ctctcgatgg
     4681 gtatcccaga accgcacgtg aaaactctga ctgcgggata cacgaaacga caagcttcga
     4741 cgctgatcga ccaacttgtt agtggagact tttcagcctt ccaggagatg aaggaggagg
     4801 aactgccaat ccttaccact tctgggacag taccagggga ggaggaaccg gagttctagg
     4861 ccactgtcaa ccaggcaaag ggaatgtgag agtgcctcaa gggcggctca aggccgtcct
     4921 tgagcactta tgacacgagc gcaacatatt ccgggacacg cttactcatg ggaagcaggt
     4981 aggcgcgcgc catccgtcac tgaaagactt ccggacgcct ccccaaagcg caacaattct
     5041 tcctgcgttt ctgggcgtaa agacccaaat atttgaagga tat
//
LOCUS       DAOQEW010000243         5039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6732_length_5039_cov_7.263644, whole genome shotgun sequence.
ACCESSION   DAOQEW010000243 DAOQEW010000000
VERSION     DAOQEW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6732_length_5039_cov_7.263644"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1301
                     /gene="glgC"
                     /locus_tag="PLS35_17590"
     CDS             81..1301
                     /gene="glgC"
                     /locus_tag="PLS35_17590"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNI20537.1"
                     /translation="MVLAGGKGERLQPLTDQRAKPAVPFGGKYRIIDFTLSNCLNSGL
                     RQIAVLIQYKSHSLDRHIRTGWNILNPELGEYIVSVPPQQRISEEWYRGTADAVYQNL
                     FLMDNDHPEFLLVLAGDHIYKMNYADMYNLLIEKQADAIVGAIETPIADANRFGVIAV
                     DEDRRILSFDEKPEHPMHIPGDPTQAFVSMGIYLFRTDVVREQLIRDAKEGTKHDFGK
                     NIIPRMIKECRVYAFKFQDENKKAVNYWRDIGTLDAYWEANMDLVSVDPQFNLYDQHW
                     PIRTYQGQFPPAKFVFAQDFQGGRMGVALDSIVCGGCIISGGRVQNSVLSPHVRVHDH
                     ADVRESVVMENVVIGEHARIRRAIIDKDVVIPPKTVIGFDPVADRQRFKVTDSGLVVI
                     SKGMKLHAAVDPSG"
     gene            1276..3138
                     /locus_tag="PLS35_17595"
     CDS             1276..3138
                     /locus_tag="PLS35_17595"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNI20538.1"
                     /translation="MPPSIHPVDLVATLRQRNLTPAIIFLTSRRACDEAMQSFERSQV
                     TLPKAQQEQIARVLTQVTEQFPSIAEHPLIETVQRIGVAAHHAGHLPSWKIAVEELMR
                     TGSLDAVFATTTLAAGVDFPARTVVVTQSSIRKARDFTDLTASEIQQLAGRAGRRGKD
                     LVGFAVITPSPYIDLTVLTKGLTGQPEAIHSQFVISYPMVLNLLKAHPREQIQSILAK
                     SFAQFQLNQRTAVLETKLDSLRAEMEPFGPRVCSDWITQWQVFEQARKRKKPRSPFTR
                     QESPDVAARLHFLTPGRVVGVTRGRGIILRQYRSKGQHAPMVTLLREGGSLNECPASI
                     VTQVFDRTFDCEETSTYPWCTPQALEQLTHHLTDLPPRLPILPILAHQHEETPIDSSI
                     IQTLGDFPCPTCPSRQACQRDFPRASRVRQEIQRHIKSIQALQTSLWHRFQERIDVLE
                     KFGYLHANAQLTADGEWARLIRIDHSLLITELIRAEAFGAIDPPLLAAVMASIAHDDD
                     RPGSFPRGSAGLVTLLFQVRKLAESLTPHEAPPMLRADVAALTERWVSDQTLTWIGLA
                     RLTTMAEGDMFRLFARTIEFLSQLKTLKATHPSLAESADHAITAMRRGVLEELP"
     gene            3135..4205
                     /locus_tag="PLS35_17600"
     CDS             3135..4205
                     /locus_tag="PLS35_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNI20539.1"
                     /translation="MNTHELERLLMQQPVSLLHRLARGRISRHFRSGKRKLVDLLLQA
                     SAENRPGLESDLQTLIDERAQRPQPVPVEKTRPPEARSRQAVPATSRPHRTDDHGHHH
                     EPPVSLHEWLSGIGVPPPQPFVPDAWQVEALAKLAETDVVVSVPTGSGKTYVAIEATA
                     RAMRENRTVIYTSPLKALSNTKFTEFSRVFGPGQVGILTGDRREHSQAPLLIMTTEIL
                     RNLLYDAAGGEIDVRLDTLGLVIMDESQYLADPERGVVWEETLIFCPSQARLLLLSAS
                     IGNPQDIADWLTAIRPSPCSLIRHTKRSVPLRAGYLHPNEKLTPLFRTAGIPYGQPHL
                     LHPEAKRLFMEYEEETGPSRSR"
     gene            complement(4389..>5039)
                     /locus_tag="PLS35_17605"
     CDS             complement(4389..>5039)
                     /locus_tag="PLS35_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20540.1"
                     /translation="SGEHRFSRTYSDIGGDNLWYLNGDVGATAHTFQDNKGTLGVFVG
                     LQYWRERHVATGVTQAECTTASAPSSEFRCSPAGTVGFRNQAVITNTTTWFSGRLGGE
                     LEYKFDRRISVEAKVAMLLSYLDNEDVHHLRTDLAQDPSFRMTGLGIGTTSDFNLGVK
                     LWDRLYLTGGYRLWWNRVLVNDQWKLYGSDGSTASAPLTQFQTLRHGPTVGLTYTF"
BASE COUNT         1057 a         1591 c         1395 g          996 t
ORIGIN      
        1 ttgaagtcaa tcattctgcg tcctttctat tcccccttcg tcctgctaca atcggcgcat
       61 ctatggcgaa catcttcacg atggtgctgg cgggagggaa aggcgaacgg ctccagcctc
      121 tcaccgacca gcgcgcaaaa cctgcggtcc ccttcggcgg taaatatcgc atcatcgatt
      181 tcacactgag caactgcctg aactcgggtc tccggcagat cgccgtgctg atccagtaca
      241 aatcacattc tctcgaccgg catatccgga cgggatggaa cattctcaat cccgaactcg
      301 gcgaatacat cgtgtcggtt cctccgcagc agcggatcag cgaagagtgg tatcgcggaa
      361 ccgccgatgc ggtctatcag aatctcttcc tgatggataa cgatcacccc gagtttctcc
      421 tggtgctggc cggcgatcat atctacaaga tgaactacgc cgatatgtac aacctcctga
      481 tcgaaaaaca ggcggatgcc attgtcggcg caatcgaaac cccgattgcg gatgccaacc
      541 ggttcggcgt gatcgcggtg gatgaagacc gccgcatctt gagcttcgac gagaagcccg
      601 agcatccgat gcacattccc ggagatccga cccaggcctt cgtctccatg ggcatctatc
      661 tgttccgcac ggatgtcgtc cgagagcagc tgattcgcga tgccaaggaa ggcaccaaac
      721 acgatttcgg caagaacatc attccgcgga tgatcaagga atgccgcgtc tatgccttca
      781 aatttcaaga cgagaacaag aaggccgtga actactggag agacatcggt acgctcgacg
      841 cctattggga agccaatatg gatctcgtct ccgtcgaccc gcaattcaac ctgtacgacc
      901 agcattggcc catccgcacc taccaagggc aatttccccc tgccaagttc gtcttcgcac
      961 aggactttca gggcggccgg atgggggtcg cattggattc gatcgtctgc gggggctgca
     1021 ttatttcagg cggccgcgtg cagaactcgg tgctgtcgcc gcacgtacgt gtgcacgatc
     1081 atgccgacgt acgcgagtct gtcgtaatgg aaaacgtggt catcggcgaa catgcccgta
     1141 tccggcgcgc catcatcgac aaggatgtcg tgattccgcc taaaaccgtg atcgggttcg
     1201 acccggtggc ggatcgtcaa cgtttcaaag tcaccgattc gggattggtg gtcatctcaa
     1261 agggaatgaa attgcatgcc gccgtcgatc catccggttg atctggtcgc gaccctccgc
     1321 caacgaaacc tcacccctgc gatcattttt ctcacctcga gacgggcctg cgacgaagcg
     1381 atgcaatcct tcgaacgcag tcaggtgacg ctgcccaaag cgcaacagga acagattgcc
     1441 cgcgtgctga cgcaggtgac cgagcagttt cccagcattg ccgaacatcc gctgatcgag
     1501 acggtccagc gcatcggtgt ggccgcgcac catgccggac atttgccctc ctggaaaatt
     1561 gccgtcgaag aattgatgcg cacgggatcg ctggatgccg tctttgccac gaccacgctc
     1621 gcagccggcg tggactttcc tgcccgcacg gtggtggtca cgcaatccag cattcgtaaa
     1681 gcgcgcgatt ttaccgatct caccgcgagc gaaatacagc aattggccgg ccgagccggt
     1741 cgccgcggca aagatctcgt cgggttcgcg gtcatcaccc cgtctcccta tatcgatctc
     1801 acggtcctga ccaagggcct gacggggcag ccggaggcga tccacagcca attcgtgatc
     1861 agttacccca tggtcttgaa cctgctcaag gcgcatccac gcgaacagat tcaatccatc
     1921 ctggccaaaa gctttgccca atttcagttg aaccagcgca cggccgtgct cgaaaccaaa
     1981 ctcgatagcc tgcgcgccga aatggagccg ttcggtcccc gtgtctgctc agattggatt
     2041 acgcagtggc aggtgttcga gcaggcgcgg aaacgcaaga agccccggag cccgttcacc
     2101 aggcaggaat cgcccgatgt tgcggcgcgc ctccacttcc tgacgcccgg gcgcgtcgtg
     2161 ggtgtcaccc gtggacgcgg gatcatcctg cgccagtacc gcagcaaagg ccaacatgcg
     2221 ccgatggtca ccctcctgcg ggaaggtggc tcgctgaatg aatgcccggc ctccatcgtg
     2281 acacaggtct tcgatcggac gttcgactgc gaagagacat caacctatcc ctggtgtacg
     2341 ccgcaggcgc tggaacaact gacccatcat ctgaccgatc ttccgccgag gctgccgatc
     2401 cttccgattc tcgcgcacca gcacgaagag acgccgatcg acagttccat cattcaaacg
     2461 ctgggggact tcccctgccc gacctgcccc tcgcgccagg cctgtcagcg tgatttccca
     2521 cgcgcctcgc gggtgcgcca ggagatccaa cgccatatca aatcgattca ggccctccag
     2581 accagtttgt ggcaccggtt tcaagagcgt atcgacgtcc tggaaaaatt cggctacctc
     2641 catgccaatg cccagctcac cgccgacggg gaatgggcgc ggctgattcg gatcgatcat
     2701 tcgctgctga tcaccgaatt gatccgggcg gaagccttcg gcgccatcga tcccccgctc
     2761 ctggcggccg tcatggccag cattgcccat gacgacgatc gtcccggatc gtttccgcgt
     2821 ggaagcgccg ggctcgtgac cttgctgttt caagtgcgga agctggcgga gagcttgacg
     2881 ccccacgaag cgccgccgat gttgcgggcc gacgtggcgg cgctcaccga acggtgggtt
     2941 tccgatcaga cgctcacctg gatcggactg gcccgcctga cgacgatggc ggaaggcgac
     3001 atgttccggc tcttcgcgcg tacaatcgag tttttgtccc aactgaagac attaaaagcg
     3061 acccatcctt cgctggcgga atcagccgat cacgcgatca cggcaatgcg ccggggtgtg
     3121 ctggaggaat tgccgtgaac acccacgaac tcgaacggct gctgatgcaa caaccggtct
     3181 ctctgctcca tcgattggcg cggggacgga tcagccggca tttccgttcc ggaaaacgca
     3241 agctggtcga tttgctcctt caagcgtccg ccgaaaatcg cccgggactg gagtccgacc
     3301 tgcagaccct catcgacgaa cgggcgcagc gcccacaacc agtgcccgtc gaaaaaacac
     3361 gaccacctga agcacgatca cgccaagccg tccctgcgac gtcgcgtccg caccggactg
     3421 atgatcatgg ccatcatcac gaaccgcctg tctcgctgca tgaatggctg tcagggattg
     3481 gcgtgccgcc gccgcaaccg tttgttcccg atgcgtggca ggtggaggcg ctggcaaaac
     3541 tggcagagac cgatgtggtc gtgagcgtcc cgaccgggag cggcaagacc tacgtggcga
     3601 ttgaagcgac cgctcgcgcg atgcgcgaga atcgcaccgt catctatacc tcccctctca
     3661 aggcgctttc caacacgaaa tttaccgagt tctcacgcgt attcggccct ggccaggtgg
     3721 gcatcctgac cggcgaccgc cgcgaacata gtcaggctcc cctcttgatc atgacgacgg
     3781 agatcctgcg caatctgctg tatgacgcag ccggcggaga gattgatgtg cggttagaca
     3841 cgctgggact ggtcattatg gatgagtccc aatacctggc cgatcccgaa cgaggagtgg
     3901 tgtgggaaga aaccttgatt ttttgcccct cgcaggctcg cctcctgctc ctctccgctt
     3961 caatcggcaa tccgcaggac attgccgact ggttgaccgc gatccgcccg agcccctgct
     4021 cgctcattcg acacaccaag cgatcagttc cgctgcgggc cggttatctt caccccaacg
     4081 agaagctcac gcctctgttt cgaactgccg gcattccgta cggtcagccc catctcttgc
     4141 accccgaagc caaacggctg ttcatggaat acgaagaaga gaccggcccc tcccgctcac
     4201 gctgaccctc aggcgccgaa gaaccggtgc cgttccagcc ctttccgctt tgcagtcggc
     4261 cggcaaagtc ttctgatcaa cggtcagctg tgggatctga gggcagggtt ctcgtcgaac
     4321 gcatcctgta cgtggggctg tgcgaggagg cggcagaaat cgacgtcacg ggagagcctc
     4381 ttcgtccctt agaatgtata ggtcaggccc accgttgggc catgtcgaag cgtttggaat
     4441 tgggtcaagg gcgcggaggc cgtagacccg tctgacccgt acaatttcca ctgatcattg
     4501 acgagcacgc gattccacca caggcggtaa ccacccgtaa ggtacagacg atcccagagc
     4561 ttcactccca gattaaagtc cgacgtggtg ccgattccca ggccggtcat tcgaaaactt
     4621 ggatcttgcg caaggtccgt gcgaaggtgg tgtacgtcct cattatctaa atacgacaac
     4681 agcatcgcca ccttggcctc gacgctgatc cgacgatcga acttatattc cagctctcca
     4741 ccaaggcgac cagaaaacca tgtggtcgtg ttggtaatga ctgcctgatt gcgaaatccg
     4801 accgttccag caggagaaca gcgaaactcc gaactgggtg ctgaggcggt cgtacattcc
     4861 gcctgagtga cgcccgtggc cacatgccgt tcccgccaat actgtaatcc cacaaacacc
     4921 cccagggttc ccttgttgtc ctggaaggta tgggccgtcg cgcccacatc gccattgagg
     4981 taccagagat tgtcaccgcc gatatcgctg taggttcgcg aaaaacgatg ttctccgct
//
LOCUS       DAOQEW010000244         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6780_length_5019_cov_7.751612, whole genome shotgun sequence.
ACCESSION   DAOQEW010000244 DAOQEW010000000
VERSION     DAOQEW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_5019_cov_7.751612"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /gene="dnaE"
                     /locus_tag="PLS35_17610"
     CDS             <1..1589
                     /gene="dnaE"
                     /locus_tag="PLS35_17610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNI20541.1"
                     /translation="LKPDRFEDIIAIIALYRPGPMDLIPDFIKRKQGKIPIAYEMPEL
                     EPILKDTYGVIVYQEQVMAIANKVAGFSLGQADILRRAMGKKKPEEMEKLRVQFLEGA
                     KQKKIAEKKADKLYELIQKFAGYGFNKSHAAAYAVVCYHTAYLKAHYPTEFMASLMTT
                     DMGNADKIVGYFTECRGLGIPVLPPDVNQSQKDFAVVDKSIRFGLAAIKNVGEGAVEA
                     VIESRQEDGPFRSFFDLFRRVDLRKLNKRMMEGLIKAGAFDSMGGRRSQYLAVLDQAM
                     DEGVSIQKERAVGQTSIFGDDVAGLPQNLADPALPDVPEWSQGELLKHERELTGFYIS
                     AHPLARYEAAIQLFSNTTSMQVADVPDGREVKLCGIITAVKSMLTKKGDRMAYITLED
                     LHGVIEVIAFPDLYRDHADMIVPEQVVRLTGTVDRGDKGTKLRGTKIEPLAELQNKGI
                     SRVMIRLHDGPTASTRLPMLRQVFQKYPGSSTVALIMALGGTIEARTSPLPNVKITPS
                     EHFVADIEEVLGKGAITLVT"
     gene            1627..2595
                     /locus_tag="PLS35_17615"
     CDS             1627..2595
                     /locus_tag="PLS35_17615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNI20542.1"
                     /translation="MRDYLDFEKPIRELEEKIEKLASAESPKSGTQDEIRKLRTKLAQ
                     VEHQLYTTLTPWQRTQLARHPQRPTSLDYISELSREFLELHGDRSFGDDRAIVGGFAR
                     FNDRSVMIIGHQKGKTLKERMQRNFGMPNPEGYRKALRLMRLAEKFNRPIITLIDTPG
                     AYPGIGAEERGQAEAIARNLFVMSRLTVPIISVVIGEGGSGGALALGVSDRILMLEHS
                     VYSVISPEGCAAILYDDPTKVPDAAASLRMTAQDLVGLGIVDEVIPEPLGGAHRDPRA
                     MCDRVAKALANQLYALVELPTDQLIAERDQKFRKIGVVGGLVPVGA"
     assembly_gap    2979..3078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3079..4053
                     /locus_tag="PLS35_17620"
     CDS             <3079..4053
                     /locus_tag="PLS35_17620"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI20543.1"
                     /translation="PVLNQLYMIIEKPLTATGSNWGIGGRLDFMYGYDFFLTQSNGAE
                     RRENGAQRWNAQGQHYGLAMPQAYAEIGNQTFSVKVGHFYTIIGYEGVPSINNFFYSK
                     SFAYQFAGPFTHWGGLATWHVNDRVTLQGGVVNGWDSLDRTKDSATGLASITYAAPEN
                     LWSLSLSMVTGNEPGAVATQFETRTRYSAIFTLHPAERWEYVLHHHYAYQNQGKFDGG
                     TARWYGIDNYLYYAFADQWRAGLRFEWMRDEAGTRVGGNPVRDNPNLGPFAGSFYSVS
                     AGLNYRPHPNVLIRPEIRADWFDGQRSPYNDGLNKNQVLAAINGNLQF"
     gene            complement(4087..4677)
                     /locus_tag="PLS35_17625"
     CDS             complement(4087..4677)
                     /locus_tag="PLS35_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20544.1"
                     /translation="MTMIALPASCERALLERFLRAEAMALWAVRAAQTQQLPRHVQTF
                     LQRHETDEQDHLRQFEGMLGTMSQRPATLPTVPSQWEMLAVLLFGYEALGLEFASLLA
                     AIRPDLSDILEDEEVHVGFFEKELKAILAGGETGAQQAREAARTWWKKLPRTVDRYLG
                     DASLAPFRTELRQHILSVIRQRFVALSLLPAEQTNR"
     gene            complement(4680..4880)
                     /locus_tag="PLS35_17630"
     CDS             complement(4680..4880)
                     /locus_tag="PLS35_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20545.1"
                     /translation="MQRLIWTSDKPKQTGWYWWRGLGEDMDPLILFVDQVGYFQWPDG
                     ASQEVGLTKGEWAGPIAPPEEQ"
BASE COUNT         1055 a         1543 c         1390 g          931 t
ORIGIN      
        1 gactgaaacc cgaccggttc gaggatatca tcgccatcat cgcgctctat cgtcccggtc
       61 ccatggacct gatcccggac ttcatcaagc gcaagcaggg caagattccg atcgcctacg
      121 agatgcccga actcgagccg atcctgaagg atacgtacgg cgtcatcgtc tatcaggagc
      181 aggtcatggc catcgccaac aaggtggccg gattctcgct gggacaagcg gacattctcc
      241 gccgcgccat gggcaagaag aagccggagg aaatggaaaa gctccgggtt caattcctgg
      301 aaggcgccaa acagaaaaaa atcgcggaga aaaaagccga caaactgtac gagctgatcc
      361 agaagttcgc cggctacggc ttcaacaagt cgcacgctgc cgcgtatgcc gtggtctgct
      421 atcacaccgc ctatctgaag gcgcactacc cgacggaatt catggcctcc ctgatgacga
      481 ccgacatggg gaacgccgac aagatcgtcg gctacttcac cgaatgccgc ggcctcggga
      541 tcccggtgct gccgccggat gtgaaccaga gccagaagga ctttgccgtc gtcgacaaga
      601 gcatccggtt cggccttgcg gccatcaaga atgtcggcga aggcgccgtc gaagcggtca
      661 tcgaatcgcg ccaagaggac ggcccgttcc gatcgttctt tgatctgttc cgccgcgtgg
      721 atctccgcaa gctgaacaag cgcatgatgg aaggactcat caaggcgggg gccttcgatt
      781 ccatgggcgg acggcgttca caatatctgg ccgtgctcga tcaagcgatg gacgaaggcg
      841 tcagtattca aaaggaacgc gctgtcggcc agaccagcat cttcggtgat gacgtcgccg
      901 ggcttcccca gaatcttgcc gatccggcct tgcccgatgt ccccgagtgg agccaagggg
      961 aactgttgaa gcatgaacgt gagttgacgg gcttctacat cagcgcccac ccgctggcgc
     1021 ggtacgaagc cgccattcaa ctcttctcca acacgacgag catgcaggtc gccgatgtgc
     1081 ccgacggccg cgaagtgaaa ctctgcggca tcatcaccgc cgtgaaatcc atgctgacca
     1141 aaaagggcga tcgcatggcg tacatcacgc tggaggattt acatggcgtg atagaagtga
     1201 tagcctttcc cgacttgtac cgagaccatg ccgacatgat cgtgccggaa caggtcgtgc
     1261 gattgacagg caccgtcgat cgcggagaca agggcaccaa gttgcgcggg accaagatcg
     1321 aaccgctggc cgaattacag aacaagggaa tctcgcgggt gatgattcgc ctgcatgatg
     1381 gcccgacggc ctccaccagg cttcccatgc tgaggcaggt gtttcagaaa taccccggtt
     1441 cgtccaccgt ggcgctcatc atggcgcttg gcggcaccat cgaagcccgg acgtctcccc
     1501 tgcccaacgt gaagatcacg cccagcgagc atttcgtcgc cgatattgag gaagtgctgg
     1561 gcaaaggcgc catcactttg gtaacctagg caaacctagg caacaccctt aagggggttc
     1621 agcagtatga gagactacct cgactttgaa aaaccgatcc gcgaactcga agaaaaaatc
     1681 gagaagctgg cttccgccga gtcacccaaa tccggaaccc aggacgagat ccgaaagctc
     1741 agaaccaaac tcgcacaggt cgagcatcag ctctacacca ccctgacgcc ctggcaacgg
     1801 acccaattgg cccgtcaccc ccagcgtccc acgtccctcg actatatcag cgaactctcc
     1861 cgcgaatttc tggaactcca tggtgatcgc agcttcggcg acgatcgcgc gattgtgggc
     1921 ggattcgccc gcttcaacga tcgctcggtc atgatcatcg gccatcaaaa ggggaaaacc
     1981 ctcaaggaac gcatgcagcg caacttcggc atgcccaatc cggaaggcta ccgcaaagcg
     2041 ctgcggctca tgcgcctggc ggagaagttc aaccgcccca ttatcacgct gatcgataca
     2101 ccgggcgcct accccggcat cggagcggaa gagcgcggac aggcggaagc gatcgccaga
     2161 aacctcttcg tcatgtcgcg actgaccgtg cccattattt ccgttgtcat cggcgaaggc
     2221 ggcagcggcg gcgcgctcgc gctcggcgtg agcgatcgta ttttgatgct ggaacattcc
     2281 gtctattccg tcatttcccc cgaaggctgc gcggcgattc tgtatgacga tccgaccaag
     2341 gtgcccgacg ccgcagcgtc cctacgcatg accgcgcagg acctcgtggg actcggcatc
     2401 gtggatgaag tgattccaga gccactcggc ggtgcccacc gcgacccgcg ggccatgtgc
     2461 gatcgcgtcg ccaaggcgct ggccaatcag ctgtacgcgc tggttgaatt gccgaccgat
     2521 caactcatcg ccgaacgcga ccagaagttc cgcaagatcg gcgtcgttgg cggacttgtc
     2581 cccgtaggag cctagtcacc ctcgaattcc ccgccccgtt cgttcctacg cgattctgac
     2641 aagccgcaag aactcgctcg cttccccgtc gctatccgac gattatttgg tgtcgcctct
     2701 cggcatcacc atactcagcc tgctcaggcg ttcgtcatgc tccgcatccg aatccggcta
     2761 tcaagcctcc ctgaaaccac ctacagcctt ggcctgattt tccatcgtcc ggcgagttgg
     2821 tacactgccg cccgcccgcg cagggtcatc actgaacagg ccatgggaga ttttcacagg
     2881 aggtgtggac aggtatgtcg aagtcgatcg tgaacgtatg catcgtgtcg ttgttatgca
     2941 cctggggcgc gtggaccgga gacgcatggg gacagtcann nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnncc cgtcttgaac cagttgtaca tgatcatcga gaaaccgttg
     3121 acggccacgg ggagcaattg gggcattggc gggcgcttgg atttcatgta cggctacgat
     3181 ttcttcctga cacagagcaa cggcgcagag cgacgggaga acggcgcgca gcgttggaac
     3241 gcgcagggcc agcattacgg actggcaatg ccccaggcct acgcagaaat cggcaaccag
     3301 acgttttcag tgaaggtcgg ccacttctac acgatcatcg gctatgaagg ggtgccgtcc
     3361 atcaataact tcttctactc caagtctttc gcgtatcaat tcgcgggccc cttcacccac
     3421 tggggcggct tggccacctg gcatgtgaac gaccgggtga cgctgcaagg cggcgtggtg
     3481 aacggctggg attctctgga tcggacaaag gacagtgcca caggtctggc cagcatcaca
     3541 tacgccgcgc ccgagaattt gtggtcactt tccttgtcga tggtcaccgg aaacgaaccc
     3601 ggcgcggtcg ccacacaatt tgaaacccgc acccgctata gcgccatttt caccctgcac
     3661 ccggctgaac ggtgggaata tgtgttgcat catcactatg cgtatcagaa ccagggcaag
     3721 ttcgacggag gcaccgcacg ctggtacggc atcgacaatt acctgtacta cgcctttgcc
     3781 gatcagtggc gagcgggcct ccgcttcgag tggatgcggg acgaggccgg tacgcgggtc
     3841 gggggaaatc cggtgcgtga caacccgaat ctcgggccgt ttgccggctc tttttattcg
     3901 gtcagcgccg ggctgaacta ccgcccgcac cccaatgtgc tgattcgccc ggaaattcgt
     3961 gccgattggt tcgacgggca gcgctcgcca tacaacgatg gcctgaacaa aaatcaggta
     4021 ttggcggcga tcaatgggaa cctgcagttc tagtgtggga tggcatggag caggcaggcc
     4081 ggagtgttac cggtttgtct gctccgcagg cagcaggctc aacgcgacga agcgttgccg
     4141 gatgaccgac aatatatgtt gccggagttc ggttcgaaaa ggcgccagcg acgcatcgcc
     4201 gagatagcga tccaccgtgc gaggcaactt cttccaccag gtacgcgcgg cctctcgcgc
     4261 ttgctgtgca cccgtctctc caccggccag aatcgccttc aattccttct cgaagaatcc
     4321 cacgtgcacc tcttcgtctt ccaggatgtc ggaaagatcc ggcctgatcg cggccagcaa
     4381 cgaggcgaat tccagcccca gcgcttcgta cccgaacaac agcactgcca gcatttccca
     4441 ctgcgacggc accgtcggca atgtggccgg ccgttgagac atcgtcccga gcatgccttc
     4501 aaattgccga agatgatcct gttcgtccgt ctcatgccgc tggagaaagg tctgcacgtg
     4561 gcggggaagc tgttgcgtct gagcggcgcg caccgcccac aaggccatgg cttcggcccg
     4621 caggaatcgt tcgagcaagg ctcgttcgca ggaggccggc agcgcgatca tcgtcacact
     4681 cactgctctt ccggcggcgc gatcggaccg gcccattcgc ccttggtcaa cccgacttcc
     4741 tgcgacgccc catccggcca ctgaaaatag cccacctggt cgacaaacag aatgagcgga
     4801 tccatatcct ctcccagccc acgccaccaa taccaacccg tctgcttcgg tttgtcactc
     4861 gtccatatca atcgttgcat cgtccgttac actcctgatc tcgcgcgacc tctgattggc
     4921 actcggcgcg cagcccccgt ttctgctaca gtcccgttga cgcgtcattg cagcgcgcac
     4981 cctaccatgg accgattacc aatagaagag gcgattccc
//
LOCUS       DAOQEW010000245         5008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6813_length_5008_cov_9.811427, whole genome shotgun sequence.
ACCESSION   DAOQEW010000245 DAOQEW010000000
VERSION     DAOQEW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6813_length_5008_cov_9.811427"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLS35_17635"
     CDS             <1..696
                     /locus_tag="PLS35_17635"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HNI20546.1"
                     /translation="GGQKPLYRALAELALGYGLWEQFQYRQAWEKLKTSLKALDMASI
                     WGGPPGLKALLPLIKANSGFLEKMVLDPAEVKEGVVLDLLAHAHRRAHGDHDPERAMV
                     ALVRALEACAQRQLFKQYKIKTWDVQPDQLPEALRDTCRTSYLDDVDGKYKLPLQSQW
                     RTLAGLGDQMGQAFLRDWPKMKPLLDAANHAVLGHGFEAVKGERVQQLSDMVMKLTGV
                     SESSLPKFPVLSL"
     gene            1137..1538
                     /locus_tag="PLS35_17640"
     CDS             1137..1538
                     /locus_tag="PLS35_17640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HNI20547.1"
                     /translation="MDIRIYYEDTDCGGVVYYANYLKYFERARTHYLEERGLSVAGLR
                     DQGTQFVVVHAELDYRSPGRYGDTLVIDTTLTDASQASFTFAHVLRERTSGRIVVEGS
                     AKLVTVDEQLKPKRLDKLTLAALQGPPHTRH"
     gene            complement(1691..2431)
                     /locus_tag="PLS35_17645"
     CDS             complement(1691..2431)
                     /locus_tag="PLS35_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="platelet-activating factor acetylhydrolase IB
                     subunit"
                     /protein_id="HNI20548.1"
                     /translation="MHRNTLSVSFLPALLFGALQLFLEPTAGSAEQIRAAMSAPQPEA
                     WWMERHERTVARIRQGHVDLLFIGDSITQGWEEDGRPVWNAFYQHRHAANLGYNGDQT
                     DNVLWRLQNGELDGITPKLAVVMIGTNNATRREDPPEETAAGIQAILTTLRTRLPDTK
                     ILLLAVFPRGLSADDRLRRVNQAVNERLPAFADQRQVFFLNLNRRFLDDNGRLPEEMM
                     PDALHPGERGYRVWAEGMEDTIKKLMGE"
     gene            complement(2587..3897)
                     /locus_tag="PLS35_17650"
     CDS             complement(2587..3897)
                     /locus_tag="PLS35_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNI20549.1"
                     /translation="MTSLEAFTSHPTVEAAVRDAQTATEGKHLSDIVDDHGFQYVDFV
                     MEGGGVLGIALTGYTYVLEQAGIRFLGIGGTSAGSINALMIAALGPPEEAKSERLLHI
                     LAGMPMASFIDGDQDARDFSQAALGKVGMMKLLLKAMQVVDNLSDDLGLNPGTAFYDW
                     LTAQLLNVGVRTNADLAKRLQTVPAGLHLRPDRTDRQLTEADTIGRLIMVAADITTET
                     KVEFPRMAELYWAKPDEVNPAYFARASMSIPLFFHPFRVNNCSQDRAKWKDVASYDGV
                     PPDTVMFMDGGIMSNFPINLFHQPYHVPASPTFGAKIGTDRAQPAVIDKPSQLLGAVF
                     DAARHTLDFDFISRNPDYKHLVAMIDTGDHNWLNFSMEDTDKIDLFARGAAAAAEFLQ
                     TFNWTKYKSIRKGIADAFLASNTNNGGSLKLAGNSQRLVALSSH"
     gene            complement(3937..4446)
                     /locus_tag="PLS35_17655"
     CDS             complement(3937..4446)
                     /locus_tag="PLS35_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20550.1"
                     /translation="MAGKIFSALLASGFPNSIRLCGKVMVSEENADARSMSQPPEGRA
                     SGRPAKGNVVALDRLFPFLYGGVLAASLWWIWMTLGRPFHPGWIAAPLLVTTIADWLE
                     GLMLLAQLRHYMSPNQQYAESLLIRLSGCATIIKLWLTSGLYVTLAGLVLNLLFTFPG
                     RRLASDAAE"
     gene            complement(4664..>5008)
                     /locus_tag="PLS35_17660"
     CDS             complement(4664..>5008)
                     /locus_tag="PLS35_17660"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNI20551.1"
                     /translation="AERNRWELAHWGNQAIPYEDDGEAVILAFKDGKVSGQAGCNRYA
                     AAITFGPKTGEVSVSHGITTRMACQPKRMEFEAAFIRAFEASTRYRLDGESLLFESAI
                     AQPLEFYRRPPE"
BASE COUNT         1101 a         1358 c         1494 g         1055 t
ORIGIN      
        1 ggcggacaga aaccgttgta tcgcgccctg gctgaacttg cactgggata tggactctgg
       61 gagcagttcc agtatcgtca ggcctgggaa aagctgaaga cgtcattgaa agccctcgac
      121 atggcgtcga tctggggtgg gccgccgggt ctgaaagcat tgctgccgtt gatcaaggcc
      181 aatagcggct ttttggaaaa gatggtgctc gaccccgccg aggtcaaaga aggtgtcgtg
      241 ctcgatctgc tggcgcacgc gcatcgccgc gcgcatgggg accatgatcc ggaacgggcc
      301 atggtggcac tcgttcgtgc gttggaggcc tgcgcgcagc ggcagttgtt caaacagtac
      361 aagatcaaaa cgtgggatgt gcagcctgat caattgcctg aggccttacg ggacacctgc
      421 cgcacctctt atctcgacga tgtcgacggc aagtataagc tgcctttgca aagccagtgg
      481 cggacgttgg ccggcctcgg cgatcagatg gggcaggcgt ttctccgcga ctggccgaag
      541 atgaaaccac tgctagatgc cgccaatcat gcggtgttgg gccatgggtt cgaggcggtc
      601 aaaggggagc gggtgcagca gctctccgat atggtcatga agcttaccgg cgtgagtgaa
      661 tcctcgttgc ccaagttccc cgtcctcagt ctctagttaa ccgtcgttcg tgaagcgtga
      721 agcgtatctc gttggaccag acagccggat ggaaaagata cgaaattttc gcgatcttga
      781 aatatggaag ctgggcaaga atatcgtgct cgatgtctat cgagtgacca aggcgttccc
      841 caaggaagag atctacggat tagtgtcgca aatgcgtcgc gcagcgatat cgattccctc
      901 gaataatagc agagggattc aatcggaaac acaatgcgga ataccgacag tttctctatg
      961 tcgcgcttgg gtcttgcgct gaattggaaa cccacgtgga aatcgctcag gacctcacat
     1021 tcatcacggt tgttcagcga gacgagctgt tggagcaact tgaccacgag tcgaagatgg
     1081 tcagaagtct tatcaaacgg ctttgacgag atacgcttca cgagatacgg aagacgatgg
     1141 acatccgcat ttactacgaa gacaccgact gcggcggggt ggtgtactac gcgaattatc
     1201 tcaagtactt tgaacgggcg agaacgcact atctcgaaga gcgaggattg tcagtcgcag
     1261 ggctgcgtga tcagggcaca cagttcgttg tggtgcatgc cgaactggat tatcggtcac
     1321 ctggacggta cggcgacacc ttagtgatcg acaccacgtt gacggatgcg agtcaggcgt
     1381 cgttcacctt tgcgcatgtg ttgcgggagc ggacgagcgg gcgtattgtg gtggaaggat
     1441 cggccaagct ggtgacggta gacgagcagt tgaaaccgaa gcggctcgac aaactcaccc
     1501 ttgctgcgtt acaaggacct ccacacacga ggcactaatg gataaactcg gcacatggct
     1561 ggcggtgacg ggcgtggcga tcggtttcgt cgtcctcgca tggctgctct tctctgataa
     1621 tccttccgac aagaaaaaga aaaaacctta gttcggatcc tgcgacggcg cgggtccggc
     1681 ttcgcattcg tcactcgccc atcaatttct taatcgtgtc ttccattccc tccgcccata
     1741 cgcgatagcc gcgttcacca gggtgcagcg cgtcgggcat catctcttca ggtaaacgtc
     1801 cgttgtcgtc gagaaagcgc cggttcaggt tcagaaagaa cacctggcgc tggtcggcga
     1861 aagccgggag ccgctcgttg actgcctggt tcacgcggcg aagccgatca tcggccgaaa
     1921 ggcctcgcgg aaagacggcc agtagcaaga tttttgtgtc cggcaaacgg gtgcgcaagg
     1981 tcgtaagaat ggcctggatt ccggcagccg tctcttccgg aggatcctcg cgcctggttg
     2041 cgttgttagt cccgatcatc acgaccgcca gctttggagt gatgccatcc agttccccgt
     2101 tctgaagccg ccacaacaca ttgtctgtct ggtcaccgtt gtagccgaga ttcgccgcat
     2161 gccgatgctg gtagaaggcg ttccagacgg gacgcccgtc ctcctcccat ccctgggtga
     2221 tggaatcgcc gataaacagc aaatcgacat ggccctgacg tatccgggcg acggtgcgtt
     2281 catgccgttc catccaccag gcttcgggct gaggggcaga catcgcagcg cggatctgtt
     2341 ctgctgatcc agcagtcggc tcaagaaata attgcagagc tccaaacagc agggcgggca
     2401 ggaaagagac ggatagtgta ttgcggtgca tagaggtccc cacaagatcc tgccccatgt
     2461 gtatcgcagg atcacgagga gatcaactgt gcctagtcgc gcattgcggt acagggatgg
     2521 cgtgaaggag gcgatgttga ctccgcctcc tccagactgc cggtattgtc cctggctcag
     2581 cacgacctag tgagagctga gtgcgacgag cctctggcta ttgccagcaa gtttcagaga
     2641 tcctccattg ttcgtattgc tcgcaagaaa ggcgtcggca atacctttgc gaatggattt
     2701 atatttcgtc caattgaatg tctgcaaaaa ttcggcggcg gctgccgcgc cgcgagcgaa
     2761 gaggtcgatc ttgtctgtgt cctccatgct gaagtttaac cagttgtgat cgccggtatc
     2821 gatcatggcc accaggtgct tgtagtccgg gtttcgcgag atgaaatcaa aatccagggt
     2881 gtgacgagcg gcatcgaaca cggcgccaag tagttgtgag ggtttgtcga tgacggccgg
     2941 ctgcgcgcgg tcggtgccga ttttcgcgcc gaatgtggga gaagccggca catgataggg
     3001 ctgatgaaac agattgatgg gaaagttgga catgatgccg ccgtccataa acatgaccgt
     3061 gtcaggaggg acgccatcat atgacgccac gtctttccac ttcgccctgt cctgggagca
     3121 gttgttgacg cggaatgggt gaaagaagag cggaatcgac atggaggcgc gcgcgaaata
     3181 ggcgggattg acctcgtcag gtttggccca atacagttcc gccatcctgg gaaactcgac
     3241 ctttgtctcg gtcgtaatat cggcggccac catgatgagt cggccaatcg tgtctgcttc
     3301 ggtcagctgc ctgtctgtcc ggtctggacg aagatgcagc cctgccggaa ccgtttgtag
     3361 ccgcttggcc aaatccgcat tggttctgac cccgacgttc aggagttggg cggtcagcca
     3421 gtcatagaag gccgtgcccg gattcagtcc caagtcatca gaaaggttgt cgacgacctg
     3481 catggccttc aagagcagtt tcatcatgcc gacctttcct agagcggcct gagaaaagtc
     3541 gcgcgcatcc tgatcgccgt cgatgaagct cgccatcggc atcccggcca gaatgtgtaa
     3601 cagccgttca ctcttggctt cctccggcgg gcctaaggcc gcaatcatca gcgcattaat
     3661 cgatcccgct gaggttccac cgatgccgag aaagcgaatg ccggcttgct ccaacacata
     3721 ggtgtatccg gtcagcgcaa tgccgagtac ccctccgcct tccatgacga agtccacata
     3781 ttggaacccg tgatcatcga caatatcgga caggtgcttt ccttcagtgg ccgtctgagc
     3841 gtcccggacg gccgcttcta cggtgggatg gctggtaaac gcttcaaggc tggtcataga
     3901 agcctccata gacaggtccc gggcgggaac gccggattat tcagcggcat cactcgccag
     3961 gcgccggccc gggaatgtga atagcaagtt cagaacaagt ccggccaggg ttacgtagag
     4021 gccgctggtg agccatagct tgatgatcgt ggcgcaaccg gaaagccgga tcaacaggct
     4081 ctcggcatat tgctgattcg gcgacatgta gtggcgaagt tgcgccagga gcatgaggcc
     4141 ctccaaccaa tccgctatcg tggtcaccag gagcggtgcg gcaatccagc ccggatggaa
     4201 cggtcgcccc agcgtcatcc agatccacca caggcttgcc gcaaggaccc ctccatatag
     4261 aaatggaaag aggcggtcca gagcaaccac atttcctttg gccggtctcc ccgaggctct
     4321 cccttccggt ggctgagaca tggaacgggc atctgcattc tcctcagaaa ccatgacctt
     4381 gccgcacagc cgtatggaat tcgggaatcc ggaagcgagg agcgcgctga agatcttgcc
     4441 agccatgata gggattccaa gttcacccat gtatcgagca agagcggtgc ccggtgggtt
     4501 tatggaatgg atgtaaacgc agcggcctat tttgagagca gggaccaatg ccagcggtca
     4561 gaatcgatgt gcttctcctg agtgtcgtat cacaagcgat acgatgtcgt atcggaaggg
     4621 atacgcgaag gacagactcg atgtgaaatc agagagagat gggttattcc ggggggcgtc
     4681 gatagaactc cagcggctga gcaatggcgc tctcaaacaa caggctttcc ccatcgagtc
     4741 gataccgggt ggaggcttca aaggcacgga tgaacgccgc ctcaaattcc atgcgcttgg
     4801 gttggcaggc catgcgcgtg gtgatgccgt gcgataccga gacctcgccg gtcttgggtc
     4861 cgaacgtgat tgccgccgca taccgattac atccagcctg acccgacact ttcccatcct
     4921 tgaaggcgag gatcaccgcc tctccatcgt cttcatacgg aatggcctgg ttgccccagt
     4981 gggcaagttc ccaacgattg cgttcagc
//
LOCUS       DAOQEW010000246         4999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6836_length_4999_cov_6.023463, whole genome shotgun sequence.
ACCESSION   DAOQEW010000246 DAOQEW010000000
VERSION     DAOQEW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_4999_cov_6.023463"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLS35_17665"
     CDS             <1..270
                     /locus_tag="PLS35_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20552.1"
                     /translation="LDLRREDQAAAKDDLRAAMQGNPHVADLFSGKRTPPARLPERFT
                     PGDRHEAALYLAGFAEAWLASSDAMEWLMEQLTMRVSRQRSPQAR"
     gene            516..1508
                     /locus_tag="PLS35_17670"
     CDS             516..1508
                     /locus_tag="PLS35_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20553.1"
                     /translation="MFHEFCFVSRAFLRNAAGSLYHIVIVALSAGIALLLPAAARQFM
                     SFWPRVEHDTWSVIAIEMAAAVLSMAGLNYIHRSIRDRGLADAALGAGLVSFFPRRGP
                     AARERIQALKDEQGTGRSVMVLGSSGAGTLVDQVGDLSSVLDKCLEAKILLVNPFNQG
                     ARARIQALAHPSCTYPAFREEVRQSIALLKRLKAIGKVVKLKLYADAPLIKLVVFGDY
                     LWLQHYHAELDVQNMPEYVLQRNHQQHGLYTLYAHYFMQRWESSELPEYDLDTDELVY
                     RSRTGQEIRREPVEIGTRHVAAHPTDTAGVDTREAFQLLHAPGPYLPVRGYDVE"
     gene            1686..2834
                     /locus_tag="PLS35_17675"
     CDS             1686..2834
                     /locus_tag="PLS35_17675"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNI20554.1"
                     /translation="MTALFSYGFRPFFLGAAWFAGLAVPAWIVMLSLGIGSTGLAAPR
                     EWHVHEMLFGFLPAVITGFVLTAVPNWTDRPAIQGRELAGLFCLWLAGRVLLAWPWVP
                     PVFAAIVDAAYLLVLAGFLWREISGGKSWSHAPIAVLLSLYAGANLLFHLFALNGTAT
                     DLPERMALGLDLTLLAFIGGRVTPNFTREYLVHARRPEKPARFTHLDMVSIGAVAFAS
                     TFWALLSQDAVAGWFLILAGVLNLVRLSRWYGWFTWREPLVFVLHWGYGWLILALVLL
                     GCAALGVGLPKEDAVHALTTGAVGVMTLGIMTRASLGHTGRQRHADAATIAMYALVTC
                     GAILRVFVAGTGLPTGLVLGAAATCWSGAYLLFALVYGPYLLRPSLDE"
     gene            complement(2878..3663)
                     /locus_tag="PLS35_17680"
     CDS             complement(2878..3663)
                     /locus_tag="PLS35_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNI20555.1"
                     /translation="MITSTFCMLPGVGLRTERQFWGQGAWTWDGFLSTRDLRGLSLAR
                     KALFDDRITEAQERFARQDSRYFAKTLAARHQWRLYEWLRERALYLDIEIDSFGQITV
                     VGLYGRGRYTALVRNESLSLLRLKEELSQYDLLVTFCGSTFDLPMLRAHFSDLPLDQP
                     HIDLCGIGRQLGYRGGLKAIERRMGITRLPAIQGFSGDDAVRQWNRWRHQRDEQARDL
                     LLAYNEADCVNLQPLADAFYCRLAVASGLEEIRLQGRTDAVSE"
     gene            complement(3754..>4999)
                     /locus_tag="PLS35_17685"
     CDS             complement(3754..>4999)
                     /locus_tag="PLS35_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20556.1"
                     /translation="GALILQMIGCLLVAATIGLMMGWLLRSFATSEKRQQLSEIATRL
                     RGREHELDTLNHELKVRTSAVQMLEGKMITSEAALKDLTADLATKVEQLSALQTELRE
                     KGVHLHAAERERDTLRREVEAAENKFKAQQAGFAEMQSQMENAEDVLVSRDQEIATLK
                     TWVEQLAPKDAEIARLRARTEELEPFQERSRRLERELEQDRARATATIQGKDQELARH
                     QAHIVEMEAELTRLRTQNQEDLTRFTETLQQRDQDIQRLRATVEELEVFRGEVEKKEI
                     QLREAEERRVMDVSEREEEIGALRKRLVEYRVAQRHQAQAKSNNQAEPASHNSSSSRS
                     AGKSRSAQKDDLKQIHGIGPVMERVLNRMGMFTFRQIAEWKDQDVEHMASELNTFPDR
                     IRHDNWIAGAKEQHFLKYGEEL"
BASE COUNT          845 a         1448 c         1537 g         1169 t
ORIGIN      
        1 cttgaccttc gacgcgaaga tcaggccgcg gcaaaggacg acttgcgagc ggcgatgcaa
       61 ggcaatcccc atgtggccga cttgttcagc gggaaacgga caccgccggc ccggttgcca
      121 gaacggttca cgccgggtga ccggcacgag gcggctctct atcttgcggg gtttgccgag
      181 gcctggctcg cgtcgtcaga tgcgatggaa tggctgatgg agcagttgac gatgcgagtc
      241 tcacgacaac ggtctcctca ggcccgttga acttctcgca agcctgccgt ccttgcgtcg
      301 cgacaacaga cctgttacag tgcagttgtt cgcgtaagac cttctgcagc cgggggcagc
      361 agcccttgga tctctgagct cttacgcgat tgttgtttgt acccgcagac gtgccgtttc
      421 cgctgagcct accgccgaag gttgttcctc cgtcgttcgc actgccagct cgtcaccgcc
      481 gtggggatct tttttacccg tctagaggtg tttcgttgtt tcatgaattc tgtttcgttt
      541 cccgggcatt cctgcgcaat gcggcaggga gcctgtacca catcgtcatt gttgccttaa
      601 gcgcaggcat tgcgcttctc ctcccggccg ccgccaggca gttcatgtcg ttctggccgc
      661 gggtggaaca tgacacctgg tctgtgattg cgattgaaat ggctgccgcc gtactgtcga
      721 tggccggctt gaactatatc catcggagta tacgggatcg aggacttgcc gatgccgcac
      781 ttggtgccgg tctcgtctca ttctttcccc ggcgcgggcc ggcagcgcga gagcgcattc
      841 aggccttaaa agacgagcag ggcaccggcc gttccgtgat ggtgctcgga tccagcggcg
      901 cggggactct ggtggaccaa gtgggtgatc tgtcgtcggt cctcgacaaa tgcctagagg
      961 cgaagattct cctggtgaat ccatttaatc agggcgcacg ggcgcggatc caggcgttgg
     1021 ctcacccttc ctgtacctat ccggcctttc gcgaggaggt ccggcagagt atcgcgctac
     1081 tcaagcgtct caaggcaatc gggaaggtgg tgaagctgaa gctctacgcc gatgctccgt
     1141 tgatcaagtt ggtggtcttc ggcgactacc tctggttgca gcattaccat gcggagctgg
     1201 atgttcagaa catgccagaa tatgtcctgc agcggaacca ccagcagcac ggcttgtata
     1261 cgttgtacgc ccactacttc atgcagcggt gggagagctc cgagttgcct gaatacgatc
     1321 tggataccga tgagctggta tatcggagcc ggaccggtca agaaatccgg cgggagccgg
     1381 tggaaatcgg gaccagacac gttgccgctc atcccacgga tacggcggga gtcgatacgc
     1441 gtgaagcgtt tcaattgttg cacgccccgg gcccgtacct gccggttcgc ggatacgacg
     1501 tcgaatagga gtcgccgcat tcatccgtcc gttcatcgcg tgctcgctgc tctcccttgt
     1561 catggctcac tcctcggcgc tgtggtatga ctagggcggc catggcaatg cctcgccggc
     1621 cggtcgtatc cacgagaggg tgaaggatga taagtcgtaa gacgaaagga agcggagtgt
     1681 cgtcaatgac cgcattattt tcctacggct tccgtccgtt ttttctcggc gccgcctggt
     1741 tcgcgggcct ggctgttcct gcctggatcg tgatgttgtc gctcgggatt ggttcaaccg
     1801 gcctggcggc acctcgcgaa tggcatgtgc acgaaatgtt gttcggattt ttgccggcgg
     1861 tcatcaccgg gtttgtgctg acggcggtgc cgaactggac agatcgtccg gccatccagg
     1921 gacgggaatt ggcgggcctg ttctgcctgt ggctcgcggg ccgtgtgctg ctggcatggc
     1981 cctgggtacc cccggtcttt gcagccatcg tcgatgcggc ctatctgctg gtgttggcag
     2041 gattcctttg gcgcgaaatt tcaggaggaa aaagctggag tcatgcgccg atcgccgtcc
     2101 tgctcagtct ctatgcgggc gcaaatctgc tttttcatct gttcgcgctc aatgggaccg
     2161 cgactgatct gcccgaacgc atggcgttgg ggctggatct gaccttgctg gccttcatcg
     2221 gcgggcgcgt gacgccgaat tttacccgtg agtatctggt gcacgctcgc cggccggaga
     2281 aacccgcccg gtttactcat ctcgatatgg tgtcgatcgg ggcagttgcg ttcgcctcaa
     2341 cattctgggc gttgttgtcc caggacgccg tggccggctg gtttctgatc ctcgccggcg
     2401 tccttaatct cgtccggttg tcgcggtggt atggctggtt cacatggcgg gagccgctcg
     2461 tgtttgtgct gcattgggga tatgggtggc tcattctggc gctcgtcctg ctcggatgtg
     2521 ccgcgctagg cgtcggcctg ccgaaggagg atgcggtgca tgcgctcacc acaggggcag
     2581 tgggtgtcat gaccttgggc atcatgaccc gcgccagctt gggacatacc ggacgccagc
     2641 ggcatgccga tgctgcgacg atcgccatgt atgccctggt cacctgcgga gcgattctgc
     2701 gagtgttcgt ggctggtacc ggtctgccta ccggtctcgt gcttggtgcg gccgcaacct
     2761 gttggagcgg cgcgtatctg ctgttcgcgt tggtctatgg gccgtacctc ttgcgtccga
     2821 gtctggatga gtagtgaagg caggttgagt aaggcaatca ccctcaggaa ggatgttcta
     2881 ctcgcttaca gcatccgtcc tgccctgaag ccgaatctcc tcgagcccgg acgccacggc
     2941 cagacggcaa taaaaggcat cggcgagagg ctgtaagttc acgcagtcag cttcgttgta
     3001 cgcgagcaag agatcccttg cttgttcatc gcgttggtgc cgccaccggt tccattggcg
     3061 gacggcatca tctccggaaa agccttggat cgccggcagg cgggtgatgc ccatccggcg
     3121 ttcgatggct ttgagccccc cgcgataccc caattgccgg ccgatgccgc agagatcgat
     3181 gtgcggctgg tccaggggca ggtctgaaaa gtgcgcccgc aacatcggaa gatcaaacgt
     3241 cgatccgcag aatgtcacga ggagatcata ttgtgacaat tcctctttca ggcgaagcag
     3301 gctcagcgac tcgtttctga cgagcgcggt atagcgtccc cggccataca acccgaccac
     3361 cgtgatctgt ccgaacgaat cgatttcgat atcgagatag agcgcccgtt cccgtaacca
     3421 ttcatacaac cgccactgat ggcgagccgc cagggttttc gcaaagtagc ggctatcctg
     3481 tcgtgcgaat cgctcttgcg cctcggtgat ccggtcgtcg aagagggctt ttctggcgag
     3541 gctgagcccg cgtaggtcgc gagtcgacag aaagccatcc cacgtccagg cgccttggcc
     3601 ccagaactgt cgttccgtgc gcaggccgac tccggggagc atgcagaagg tggaggtaat
     3661 catgtagggt ctccgaacgg gtcggtacag cgtgcagcag gaaggggctg gaataacagc
     3721 gaggagggga acggacgatc tgatcaggac ggactacagt tcctcaccgt atttcagaaa
     3781 atgttgttcc ttggcgcccg cgatccaatt gtcgtggcgg atgcgatccg ggaaggtgtt
     3841 gagctccgac gccatatgtt cgacgtcttg atccttccat tcggcgatct ggcgaaatgt
     3901 aaacatgccc atgcgattta acacgcgctc catcaccggt ccaatcccat gaatctgctt
     3961 gagatcgtct ttctgggcag accgtgattt tccggcactc cgggagctgg aggagttgtg
     4021 cgaggccggc tcagcctggt tgttcgattt cgcctgcgcc tggtgccgtt gggcgacgcg
     4081 gtattcgacc agccgcttgc gtagcgcgcc gatttcttct tcccgttcac tgacgtccat
     4141 cacccggcgt tcttccgcct cgcggagttg aatctccttt ttctccacct ctccacggaa
     4201 cacctccaat tcttcgacgg tggcgcggag gcgttgaata tcctgatccc gctgctgcag
     4261 cgtttcggtg aagcgcgtga ggtcttcctg gttttgtgtc cgcaggcgag tgagttctgc
     4321 ttccatctca acgatgtgtg cctggtgacg ggcgagctcc tgatccttgc cctgaatggt
     4381 agctgtagcc cgcgcgcgat cttgttcgag ttcccgctcc aatcgacggc ttcgttcctg
     4441 gaacggttcc agttcttcgg tgcgcgcccg caggcgggca atttcggcat ccttgggcgc
     4501 aagttgctcc acccaggttt ttagtgtggc gatttcctga tcccggctga ccaacacgtc
     4561 ttcggcattc tccatctgcg attgcatttc ggcgaagccg gcttgttgcg ccttgaactt
     4621 gttttcggcg gcctcgactt cccggcgaag cgtatcccgt tcgcgctcgg ctgcatggag
     4681 gtgcacccct ttttcacgga gttcggtctg cagcgccgag agttgttcga cctttgtggc
     4741 cagatcggcg gtcaaatctt tgagcgccgc ttcggaggtg atcatcttgc cttccagcat
     4801 ctggacggcg gaggtgcgca ctttcaactc atggttgagg gtgtctaatt cgtgttcgcg
     4861 accgcggagc cgggtggcaa tttcggataa ctgctggcgt ttctccgacg tggcgaaact
     4921 ccgcaacagc cagcccatca tgagcccgat ggtcgccgcc accagtaagc atccgatcat
     4981 ttgcagaatt aaggctccc
//
LOCUS       DAOQEW010000247         4989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_6855_length_4989_cov_7.583097, whole genome shotgun sequence.
ACCESSION   DAOQEW010000247 DAOQEW010000000
VERSION     DAOQEW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6855_length_4989_cov_7.583097"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLS35_17690"
     CDS             complement(<1..320)
                     /locus_tag="PLS35_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20557.1"
                     /translation="MRLVVTIDVEEDQWGIVPHGQATAQNVYRLPRLQALLNEFGIIP
                     TYLVTYPVMQDSYAVGILREIVEAGECEIGAHCHPWNTPPFKEPLSKRNSMLCNLPAT
                     LQYE"
     gene            complement(364..1488)
                     /locus_tag="PLS35_17695"
     CDS             complement(364..1488)
                     /locus_tag="PLS35_17695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI20558.1"
                     /translation="MKQRRRLTIQVVTTPEEFKGLAGAWETLLDRMPGHSMFLTWEWL
                     YYWTLHYLGDSQLRILLAFDEHETLVGIAPLYLRCTRKHAVIAVRELRLLGSEAVCSS
                     YLDLIVQEQHKAVMLQSLYHYLFGDARDEWDLLTLSDLAAESSTLDLWNGLVAKAGKV
                     GDVSTTSCCPVIRLPADLPTYRASLGRNRRYTIQRKTRCLQQAGRMQFTRATSPADVE
                     IAFESVITLHQRRWSSRPGGGVFANEQARRFHRDIVRVLSERGRVRIDLLLLDDRPIA
                     GVYGFLYQGVYYFYLPGFDPDILPKASPGLLLLHRVIEQALCDGAQMVDLLQGSQPYK
                     LEWSTGLCRLVTSRCYNRSASGAAVTLLDGAKQALKILLR"
     gene            complement(1485..3074)
                     /gene="xrtW"
                     /locus_tag="PLS35_17700"
     CDS             complement(1485..3074)
                     /gene="xrtW"
                     /locus_tag="PLS35_17700"
                     /inference="COORDINATES: protein motif:HMM:NF038142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase W"
                     /protein_id="HNI20559.1"
                     /translation="MENSLVTPSATGTRPTSAGVHSRTPAYLLRNYSALWRITGLCLP
                     LLLLCAPVLHDLLDLWWNRYGFSHGFLVPLLSLYLVRLQWPTLKQLPVEPALGAGLLW
                     LVGAIALLLASEVAGILTTGSVALILVLAGLVLLLCGFAYLRALAFPLAYLIFMTPVL
                     DGLTEPLVWPFQLLTANMSVSMLRALGIPVILENSIDIILPTVTLEVARECSGAGLLI
                     AVLAIGLPLAALTLQTWWARVTLLLSSVAIAIMANWLRLTVMGVYAQAGGKDLHGPYH
                     ILQGLFVDWVAFAFLFAGAWLLGRLEHVPLRPSIHGEEKKCFSEPVSHRHASNRAWWL
                     ACVTLSVATLVLYGLDRGASGLKKELATFPGVVGDWVVDHRLNSASPVSLAAADDSLM
                     RTYRDSDGRRVNVYVAYTKSQRQGKELVGMETAPLHTGAIAAMLGRREDAIAVNRTVF
                     DERGRPVPSLFWYHINGSSYTDRVEAKLATIKQALLRGRTDGALVLVSAEPRSRENDD
                     PWKAQEEFVASALPLLREYLP"
     gene            complement(3116..>4989)
                     /locus_tag="PLS35_17705"
     CDS             complement(3116..>4989)
                     /locus_tag="PLS35_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20560.1"
                     /translation="SLGDLYLAGGKMDEVGKIAEELVSANPQHPAGYLLRAAVALRAE
                     RNADAIAILKQAVTLDPKMVGPHLALANIHFAQQDIEQAAEWYDRGVKADPASAGARI
                     ARGQFLFATGAPEEGRKEFHKAVELSSDQESIKLVLAERYAMLGLREDAEHELSALIA
                     DRHSQRARKALAELKLTLGEVAEAKPLLQAILAADEHDPVALYLKGRVAFAERDLLQA
                     ASLFEAAIGRDTTLVGPHVYLGLIRSTQGRMDQAEEALREAIRRDPSNQTAHLALARL
                     YLTQEKPKEAEKEVSQTLRVNPASLEAAVLYGDVFVFGKNWARAEEVYGAISRQLPGQ
                     PIGYVKMAALRQLQARPAEAAELFAQALVYAPDDPVIMQDYLVTLVGSKQSQQADRVL
                     GAYLAKTSRDPNTWRLAGRVYVAQGKIDQAQKAFRKAVDLAPDLALAHYELGQVQLLQ
                     NKLPAAVSAFQSALKKDETNSAIHTALGMVLARRGQVDAANGHYKRAFQLNPRDVVAA
                     NNLAANLSDQQELDDALGFARDALELAPSNPAIKDTLGWIYFKQGRFDRAYPYLAEAS
                     AALPQHPVVRYHHAVVLSKIGRQTEALSELKSALSLPGGFPEAEQAARMVASNKMDE"
BASE COUNT         1092 a         1554 c         1378 g          965 t
ORIGIN      
        1 ttttcgtatt gcagggtcgc cgggaggttg cagagcatgc tattgcgttt acttaacggt
       61 tccttaaacg gcggagtatt ccacggatga caatgcgccc cgatctcgca ctccccggct
      121 tccactattt cccgaaggat gccgacggca taggagtctt gcatcaccgg gtaggtcacc
      181 agatacgtcg ggatgatgcc gaactcattg aggagagcct gcaatctcgg caagcggtat
      241 acattctgcg ccgtggcctg gccatgcgga acgatccccc attgatcctc ttcgacatcg
      301 atcgtaacga ccagtctcat tattcactcc aacgcagtcg gcaacagccg tacagttgct
      361 ttgttaccgc agcagtatct taagagcctg ctttgcacca tctaacaacg tgaccgccgc
      421 accagacgca ctgcgattgt aacagcgcga tgtcaccaac cggcaaagac cggttgacca
      481 ttcgagctta tacggttgcg agccctgcaa gagatccacc atctgagcac catcgcaaag
      541 tgcctgctca atgacccgat gcaacaggag gaggcccgga ctggctttcg gcaggatgtc
      601 cggatcgaac ccgggcaaat aaaagtaata aacgccttga tacagaaacc catagacgcc
      661 tgcgatgggc cggtcatcca gcaacaaaag atcgatcctg actcgccccc gctcactcaa
      721 taccctcacg atatcccggt gaaaccgtcg agcctgttca ttcgcaaata cgccaccgcc
      781 cggccgtgag ctccaccgtc gttggtgcag ggtgatgacg gactcgaagg cgatctcaac
      841 atcggcaggg ctggtggcac gggtaaattg cattcgcccc gcctgctgga gacacctcgt
      901 ctttcgttga atggtgtatc gtcggttgcg tcccaggctg gctcgatacg tggggagatc
      961 ggccggcagc cggatgaccg ggcaacagct cgtcgtactc acatcaccga ccttccctgc
     1021 cttggcgact aatccgttcc acagatcgag cgtggaggat tcggcagcca agtctgacag
     1081 cgtcagaaga tcccactcat cccgagcatc accgaacaaa tagtggtaga ggctctggag
     1141 catcacggcc ttgtgctgct cttgcacaat gaggtccaga tacgaggaac acactgcctc
     1201 gctccccaac aatctcaatt cccgaaccgc gatcaccgcg tgtttcctgg tacagcggag
     1261 atacagtggc gcaatgccga ccaatgtttc gtgctcgtca aatgcgagaa gaattcgcag
     1321 ctgactgtca cccaaatagt ggagggtcca gtagtacagc cattcccagg tgagaaacat
     1381 gctgtgtccc ggcatcctgt ccagcaacgt ctcccacgct ccagccagcc ccttgaactc
     1441 ctcgggggtc gtcaccacct gaatggtcag tcgacgccgc tgcttcatgg gagatactcg
     1501 cgcaacaaag gcaaggccga ggcgacaaac tcttcctgtg ctttccaggg gtcatcgttc
     1561 tcccggcttc gaggctcagc ggagaccagc accagagccc cgtcggtccg cccccgcagc
     1621 aaggcctgtt taatcgtcgc cagtttagct tcgacccggt ccgtataact gctgccgttg
     1681 atgtgatacc aaaacaggga cgggacggga cgaccacgct cgtcgaaaac ggttcgattc
     1741 accgcgatgg catcttccct tctccccaac atggcagcga tcgcccccgt atgaagcgga
     1801 gccgtctcca tgcccaccag ctctttcccc tgacgttgcg acttcgtgta tgccacgtac
     1861 acattcactc gccgtccgtc agagtcgcga tacgtccgca taagggaatc atcagcggcg
     1921 gcgaggctca ccggtgaggc actgttcagg cggtggtcga ccacccaatc cccgacgacg
     1981 cccgggaatg tcgccagctc tttcttgagt cccgacgccc cgcgatcaag tccatagaga
     2041 acaagcgtcg caacggaaag agtgacacaa gccagccacc aggcgcgatt cgaggcatgg
     2101 cgatgagaga ctggctcaga gaaacatttc ttctcctccc catggataga tggccgcaac
     2161 ggaacgtgct ccagtctgcc caacaaccag gctcccgcaa acaggaacgc gaaggcgacc
     2221 caatcgacaa acaagccctg cagtatgtgg tacggcccgt gaagatcctt gcctcccgct
     2281 tgagcataga cgcccatgac ggtcaaacgc agccaattgg ccataatggc aattgcgacc
     2341 gacgacagga gcagggtgac gcgggcccac caggtctgca aggtcagagc cgccagcggc
     2401 aatccgatcg ccagcacggc aatgagcagg cccgccccgc tgcattctcg ggcgacttca
     2461 agagtgacgg taggaagaat gatgtcgatg ctgttctcca gaatcacggg gatgccgagg
     2521 gcgcgcaaca tggagacgga catatttgca gtgaggagtt gaaatggcca tacaagcggc
     2581 tccgtcaggc catcgaggac cggcgtcatg aagatcaaat aggcgagcgg aaacgcgagt
     2641 gctctcaaat aggcgaaccc gcaaagcagc aacaccagtc ccgcgagaac caggatcaac
     2701 gcgacgcttc cggtggtgag aatgccggcg acttcgctcg ccaggagtaa ggcgatggca
     2761 cctaccagcc acagcaagcc tgcgcccagc gccggttcaa ccggaagctg cttcagcgtg
     2821 ggccactgta gtctgacgag atagagactg agcagaggga ccaggaaccc atgggaaaag
     2881 ccgtaccggt tccaccacaa gtccaggagg tcgtggagta ctggcgcaca gagcaggagg
     2941 agaggaaggc agaggcctgt gattctccac agggcggaat aattgcggag cagatacgca
     3001 ggtgtcctgg agtgaacacc cgccgacgtc gggcgggttc ctgtagcgga aggagtgacg
     3061 agagaattct ccacgcgtcg gcctcactga tcatcaccac agcacagtta accaactact
     3121 catccatctt atttgacgcc accatccgcg ctgcttgctc ggcttccgga aaaccacccg
     3181 gcaatgacag ggcggatttc agctcagaca aggcctcggt ctgccgacca attttggaga
     3241 gcaccaccgc atggtggtaa cggacgacgg gatgttgtgg gagcgcggcc gaagcttctg
     3301 ccaggtatgg atacgcccta tcgaaccggc cctgcttgaa atagatccag cccagcgtat
     3361 ctttaatcgc cgggttggaa ggagccaact ccagcgcatc ccgtgcaaac ccgagtgcat
     3421 cgtcaagctc ctgctggtcg ctcaggttcg ccgccagatt attcgcggca accacatccc
     3481 gaggattgag ctggaaggcg cgtttgtaat ggccattcgc cgcatcgact tgtcctcgac
     3541 gggccaacac catgccaagt gccgtatgaa tcgccgaatt cgtctcgtcc tttttcaaag
     3601 cactttgaaa cgccgacacg gctgcaggca gcttattctg cagaagctgc acttgtccga
     3661 gttcgtagtg cgccagcgca agatccggtg ccagatccac cgctttccga aacgctttct
     3721 gtgcctggtc gattttgccc tgagcgacgt acacgcgtcc cgcgagtctc catgtgtttg
     3781 gatcgcgcga tgttttggcc agatacgccc cgagcacgcg atctgcctgc tgagactgct
     3841 tcgaccccac gagtgtgacc aggtagtcct gcatgatcac cgggtcatca ggcgcataga
     3901 ccagcgcttg cgcaaagagc tccgcggcct cagccggcct tgcttgcagc tggcgaagcg
     3961 ctgccatctt cacatagcca atcggttgtc caggcagttg cctgctgatg gcgccataca
     4021 cctcctcggc cctggcccag ttcttcccga acacgaacac atcaccatac aacacggcgg
     4081 cttccaggct ggcggggttc actcgcaacg tctgcgagac ttccttctcc gcctccttcg
     4141 gcttctcttg tgtgagataa agacgcgcga gagccagatg cgcagtctgg ttagagggat
     4201 cacgcctgat cgcctcgcgc agcgcttctt ctgcctggtc catgcggcct tgggttgatc
     4261 taatcaagcc aaggtacaca tgaggcccga cgagagtcgt atcacgaccg atcgccgctt
     4321 cgaacaggct ggccgcttgg agcagatcac gttccgcgaa tgccacccgc cccttgagat
     4381 acagggcgac cggatcatgt tcatcggctg cgagaatcgc ctgcaggagc ggtttcgctt
     4441 cggctacctc accaagggtc aacttcagct cggcgagggc cttccgtgcc ctctgagaat
     4501 gcctgtcggc aatgagcgcg gacaattcgt gttcggcatc ttcccgaagg ccgagcatgg
     4561 cataccgctc cgcgagcact agcttgatgg attcctgatc cgaactcagc tcgactgcct
     4621 tgtgaaactc cttccgcccc tcctccggag caccggtcgc aaacagaaac tgtccacgag
     4681 caatgcgagc accggcagat gcaggatcag ccttgacccc gcggtcgtac cactcggccg
     4741 cctgctcgat gtcttgttgt gcaaaatgga tattcgccag ggcgaggtgg gggcccacca
     4801 tcttcggatc gagtgtgacc gcctgcttca ggatcgcgat tgcatctgca ttccgctctg
     4861 ctctcaaggc gactgctgcg cgaagcaggt accctgccgg gtgttgggga ttggccgaca
     4921 ccagctcttc ggcgatcttg cctacctcgt ccattttccc tccggccaga tacagatcgc
     4981 cgagggacc
//
LOCUS       DAOQEW010000248         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7018_length_4925_cov_7.033470, whole genome shotgun sequence.
ACCESSION   DAOQEW010000248 DAOQEW010000000
VERSION     DAOQEW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7018_length_4925_cov_7.033470"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2151
                     /locus_tag="PLS35_17710"
     CDS             <1..2151
                     /locus_tag="PLS35_17710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20561.1"
                     /translation="QQLAEQNAEVERKNREIEQARHALEDKARELALASKYKSEFLAN
                     MSHELRTPLNSILILAQQLSENPDQNLSSKQVEFSKNIHAAGADLLNLISDILDLSKI
                     ESGTVTVEPEEVLFMKVREATERSFRHVAEARQLAFHIEIDPQLSRSMVTDFKRLQQV
                     LKNLLSNAFKFTSHGQVTFRIRRAVTGWSLDHAVLSTVPTVVAFEVSDTGIGIPVEKQ
                     KIVFEAFQQADAGTSRRFGGTGLGLAISRELAMLLGGEIRLVSAPGEGSTFTLYLPEA
                     YMGATPEMISPKFQMQEQTPLLVQQAHRLEDIPDDRETILPGDPTVLIVEDDPHYARV
                     LLGAVREKGLKGIVTAHGRLALALTREYKPVAITLDIFLPDMLGWTVLSHLKQDPETR
                     HIPVQILTVEEERMHGLGHGAFAYMIKPATTEDLGETFGRLVEFTKPRRKQLLLVEDN
                     EIESRSLCELLRHENVDIVAVNNGTDAWSQLSNRPFDCVVLDLRLPDMSGFHILERIR
                     EHDALRNLPVIVSTGKDLSEQEEIHLRELSQGIVLKGVQSPERLLDEATLFLHLVVSD
                     LPADKQRMLERVRQSDDALVGKKVLVVDDDMRNIFALTSLLERHGMEVTGCETGREAI
                     QQVEEDPDLDMVLMDIMMPEMDGYETMRHIRQNPQHRLLPILALTAKAMKGDRERCLQ
                     AGASDYIAKPVNTDELLALLRIWLFGRKKAMGKP"
     gene            2228..4309
                     /locus_tag="PLS35_17715"
     CDS             2228..4309
                     /locus_tag="PLS35_17715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20562.1"
                     /translation="MTDIQAKILLVDDDARSLMAMETLLTGPGRVVITADSGHEALRQ
                     VLRHDFAVILLDVRMPRVDGFEAAELIRQREQSRHTPIIFLSAVDKLEEDVFRGLASG
                     AVDYLFKPVVPAVLQSKVSVFVDLYRLRERVKLQAVRQGEERFRLLVDSIRDCSILLL
                     SADGAVTSWNPGTRLVEGYEADDVIGRNHSLFCTAEDIARGYPGLALDTAAAAGQHEA
                     EGWRVRKDGSTFWAHVVITAMKDETGKLSGFARVARDLTERKVAEEQLRKIAVELEQR
                     VSERTLELRESQMRLRELATELTLTEQRERRRLAGDLHDYLAQLLVLIRIKIHQISAQ
                     VKEPAPASLLMEADHAIIDALDYTRSLVAELAPPALQEFGLLEGFEWLAEQMKNHGLK
                     VTVHKHVQDVVLGADQAVLVFQSTRELLFNVLKHAGTNQATLIIDRNSDGHLVVSVKD
                     EGRGFDPHAMLARNDERKRFGLFSVRERTEAMGGKLEVTSLSNQGTCAALIVPYWPIE
                     ANQSTAPDSAVSPARSQGAPSFLSSEPANGERTNDATGESTQAGVLRIVLADDHVMVR
                     QGLRSILDSYPDISIVGEAADGEEAVELAGRLAPDVIVMDVNMPKMDGIEATRRIKAR
                     WPGITVVGLSVSTAAQVESLLLEAGASCYVSKEAAGIHLYSAILTTVPQDSACRMEKR
                     GTVLPAGETPP"
     gene            complement(4327..>4925)
                     /locus_tag="PLS35_17720"
     CDS             complement(4327..>4925)
                     /locus_tag="PLS35_17720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20563.1"
                     /translation="RMAAMGGDLLIESAPGQGTKATIRTPYRHTPIDGEEQPSAPAMA
                     LPSSNARRKDVSAPPLSRELSDRIGILLVDDHAMVRQGLRSVLDEYDDVAVIGEASNG
                     KEAVESVAQLRPAVVVMDINMPYINGIDATMQIKSRHPDIAVIGLSVQNSGETREAML
                     RAGATTLISKEAAVDELYLAIRQALAGENAESALPADR"
BASE COUNT         1040 a         1375 c         1514 g          996 t
ORIGIN      
        1 cagcaactgg ccgagcagaa tgcggaggtc gaacgaaaga atcgggagat cgaacaggcc
       61 agacatgcgc tggaggataa ggcgcgtgag ttggccttgg cctcgaaata caaatccgaa
      121 ttcctcgcca atatgtccca cgagttgcgg actcctctca acagcatcct gattctggcg
      181 caacagctca gcgagaatcc ggatcaaaat ctcagctcca aacaggtcga gttttccaag
      241 aacattcacg ccgccggcgc ggatctgctc aacctgatca gcgacatttt ggatctttcg
      301 aagatcgaat ccggcacggt gaccgtcgaa ccggaagaag tgctgttcat gaaagtgcgg
      361 gaggccacgg agcgcagttt ccggcatgtc gcagaagcgc ggcagctcgc ctttcacatc
      421 gagatcgacc cgcagttgtc ccgcagtatg gtcaccgact tcaagcgatt gcagcaggtg
      481 ttgaaaaatc tcctgtccaa cgcgttcaag tttacctctc acggtcaagt caccttcaga
      541 atccggcgcg ccgtcaccgg ctggagtctg gaccatgcgg tcttgagcac ggtcccgacc
      601 gtggtggcgt ttgaagtgag tgatacgggc atcggcattc cggtggagaa gcagaagatc
      661 gtgttcgagg cgttccaaca ggcggatgct ggaaccagcc ggcggtttgg cggtacggga
      721 ctcggcctcg ccatcagccg cgaattggcc atgctgttag gcggagaaat ccggttggtg
      781 agcgcccccg gcgaaggaag cacctttacg ctgtatctgc ctgaagccta tatgggcgcg
      841 acgccggaaa tgatctctcc gaaatttcag atgcaggaac agacgccgtt gctggtgcag
      901 caggcccatc gactggagga catacccgat gatcgggaaa cgattctccc cggcgatcca
      961 accgtgctga tcgtggaaga tgatccgcat tatgcccgcg tgctcctcgg ggccgtccgg
     1021 gaaaaggggt tgaaagggat tgtcaccgcc catggccgcc tggccttggc cctgaccaga
     1081 gagtacaagc cggtggccat cacgctggat atctttctcc cggacatgct cgggtggacc
     1141 gtcttgagcc acctcaagca ggatcctgag accaggcata ttcctgtgca gatcctcacg
     1201 gtggaagagg aacgaatgca cgggctggga catggcgcct tcgcctacat gattaaaccg
     1261 gccacgacgg aagatctcgg ggagaccttt ggccggctgg tcgagttcac caaacccagg
     1321 cgcaagcaat tgttgctcgt cgaggacaat gaaatcgaaa gccgcagcct gtgtgaactt
     1381 ctccggcacg aaaatgtaga catcgtcgcg gtgaacaacg gaaccgatgc ctggagtcag
     1441 ctgtctaata gaccgtttga ttgtgtggtc ttggacctcc ggctgccgga catgtccggg
     1501 ttccacatcc tcgaacgcat ccgcgaacat gatgccttga ggaatcttcc tgtcattgtg
     1561 tcgaccggca aggacttgag cgagcaggaa gaaattcatc tgcgggaact ctcgcaaggg
     1621 atcgttctca aaggtgtcca gtctccggaa cggcttctgg atgaagccac cttatttttg
     1681 cacctggtcg tgtctgacct gccggcggac aaacagcgca tgttggaacg ggtgcggcaa
     1741 agcgatgacg cgctggtggg caagaaagtc ctggtggtgg acgacgatat gcgaaatatt
     1801 ttcgccctga ccagcctgct cgagcggcat ggcatggagg tgaccgggtg tgagaccggc
     1861 cgtgaagcca ttcagcaggt ggaagaggat cctgatctgg acatggttct gatggacatc
     1921 atgatgcctg aaatggacgg ctatgagacc atgcgccaca tccggcaaaa cccccagcat
     1981 cgattgctgc cgattcttgc cttaaccgcg aaggcgatga agggagatcg tgaacgctgc
     2041 cttcaggcgg gtgcctcgga ttatattgcg aaaccggtca ataccgatga gttgctggcc
     2101 cttctcagga tttggctctt cggacgcaaa aaagccatgg gcaaaccatg acggagttct
     2161 ggccgattgc tgttcgtgcc acatccccat gccggcaaca ctgagctgcg ttgaagagga
     2221 gtcctttatg accgacattc aggccaagat cctcttagtg gatgacgatg ccagaagcct
     2281 gatggccatg gagaccttgt tgacgggacc tggccgtgtc gtgatcacgg cggattccgg
     2341 gcacgaggcg cttcgccagg tcttgcggca cgattttgcc gtcatcctcc tcgacgtccg
     2401 tatgccgcga gttgatggat tcgaagccgc cgagttgatc aggcaacgcg agcagtcgcg
     2461 ccatacgccg atcatctttc tctcggctgt cgataagctg gaggaggatg tgttcagggg
     2521 gctggccagc ggagcagtcg attacctgtt taaacccgtg gtccccgctg tgctgcaaag
     2581 caaggtgtcg gtgttcgtcg acctctatcg cctgcgcgag cgcgtgaaac tgcaggccgt
     2641 acggcagggc gaggagcggt ttcgtctgct ggtggatagc atcagggatt gctcgattct
     2701 cctcctcagc gcagacgggg cggtgacaag ctggaatcca gggacccgcc tggtggaagg
     2761 ctatgaagcg gacgatgtga tcggccgaaa ccattcgctc ttttgtacgg ccgaagatat
     2821 cgcgcgaggc tacccgggcc tggcgttgga cacggctgcg gctgccggtc aacatgaagc
     2881 cgaagggtgg cgagtgcgga aggacggctc cacattctgg gcccacgtcg tcattactgc
     2941 catgaaggat gagacgggga aactcagcgg ctttgcacgc gtggcgcggg acctcacgga
     3001 acgcaaagtg gccgaagaac aactccgaaa gatcgccgtt gagctggaac agcgcgtaag
     3061 cgagcgaacg ctggagctcc gcgaatctca aatgcggctc cgtgaactgg cgactgaatt
     3121 gacccttacg gaacagcgtg agcgccgccg gctggccggg gatctgcacg attatcttgc
     3181 gcagctgctc gtattgatca gaattaagat ccaccagatc tccgcccagg ttaaggagcc
     3241 ggcgccggca tccctattga tggaagcgga ccatgccatc attgacgcac tggattacac
     3301 gcgatcgctg gtcgcagaac tggctcctcc ggcgctccag gagtttggtc tgctcgaagg
     3361 ctttgagtgg ttggcggagc agatgaaaaa tcacggctta aaggtcaccg tccacaaaca
     3421 cgttcaagat gtggttctcg gggcggatca ggccgtgctg gtgtttcaat ccacgcgtga
     3481 attgctgttc aacgtgctca aacatgccgg gacgaatcag gcgaccctga tcattgatcg
     3541 aaacagcgac ggccatctgg tcgtgagcgt gaaggatgag gggcgggggt ttgatcccca
     3601 tgccatgctg gcccggaacg atgagcggaa acgattcggc ttattcagtg tgcgggaacg
     3661 aaccgaagcg atgggaggca agctggaagt cacttccttg tccaaccagg gaacctgcgc
     3721 cgcattgatc gttccctact ggcccattga agccaaccaa agcacggcgc ctgactcggc
     3781 cgtgtcgccg gcgcgatctc aaggggcgcc atcgtttctc tcttccgagc ctgctaacgg
     3841 cgagcgcacc aatgatgcaa ccggtgagtc aacgcaagcc ggggtcctgc gcatcgtcct
     3901 cgccgatgac catgtcatgg tgcggcaagg gctgcgcagt attttggaca gctatcccga
     3961 catctccatc gtgggcgagg cggcagatgg ggaagaagcc gtagaattgg ccggtcggtt
     4021 ggctcccgat gtaatcgtga tggatgtgaa tatgccgaag atggatggca tcgaagccac
     4081 gcgtcgaatc aaagcgagat ggccggggat caccgttgtg ggcctctccg tctcgacggc
     4141 cgcgcaggtt gaatcgttgc tgctggaagc gggagccagc tgttatgtct ccaaggaagc
     4201 cgccggcatc catctctaca gcgcgatcct cacgacggtc ccgcaagatt ccgcttgccg
     4261 gatggaaaag aggggaaccg ttctgcccgc cggtgagacg cctccgtagc acattcccgg
     4321 tcacgcttac cgatcggcgg gcaatgcaga ctcggcattt tcgccggcaa gcgcctggcg
     4381 gatcgcgaga tacaattcgt ctacagccgc ttctttggaa atcagcgtgg tggctccggc
     4441 gcgaagcatc gcctcgcggg tttcgccgct gttctgtacc gacagcccga tgacggcgat
     4501 gtccggatgt cgggatttga tttgcatggt cgcatcgatt ccgttgatgt aaggcatatt
     4561 gatgtccatc accaccacgg cgggacggag ctgcgccact gattccaccg cctccttgcc
     4621 gttcgacgct tccccgatca ccgccacatc gtcgtattcg tccagcacgc tccgcagccc
     4681 ttgacgcacc atcgcatgat cgtccaccag caggatgccg atacgatcgg acaattcccg
     4741 ggacagtgga ggtgccgata catccttcct tctggcattg gacgaaggga gtgccatggc
     4801 cggcgcggaa ggttgctcct caccgtcgat gggcgtatgc cgataggggg ttctgatagt
     4861 ggctttggtt ccctggccgg gcgcggattc aatgaggagg tcaccgccca ttgccgccat
     4921 tcgct
//
LOCUS       DAOQEW010000249         4916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7041_length_4916_cov_8.346636, whole genome shotgun sequence.
ACCESSION   DAOQEW010000249 DAOQEW010000000
VERSION     DAOQEW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7041_length_4916_cov_8.346636"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLS35_17725"
     CDS             complement(<1..850)
                     /locus_tag="PLS35_17725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI20564.1"
                     /translation="MSKPLEPAEQPGPVVSGNIFVRFQQGFERGFNRFRERYGLLLER
                     AMARRRAFVTVCLVIAVASLSLFFFLGRDYFPEIRSGVIQMHMRAPLGTRIEVSGRIA
                     TLVSNSVEELLAGEVENIVSNCGLPVGAHNLAFIPTPTIGSQDCDLTILLRDEKSPVW
                     EYRRILRKGLTERYPGTEFTFQPSDLTAKILNFGAPAPIDVQIKGPDMHPNYEFTRKL
                     AGRLRQIPGATDVVIQQTMRTPTLMVEGNRTFGLAVNRTQKEMASNLLMTTAGSQQID
                     QVYWLDP"
     gene            complement(834..2333)
                     /locus_tag="PLS35_17730"
     CDS             complement(834..2333)
                     /locus_tag="PLS35_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI20565.1"
                     /translation="MNEIVLIALRRPYTFVVMSILIVLLGSITVLHMPTDVFPNITIP
                     VTSVVWAYAGLLPQQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIFLQDGVD
                     VGRAEADITAIAQTVVKALPPDISPPMVMRLAPSSIPVAMLQISSDRLTPAELYNLAY
                     MRIRPLLVTVPGIVLPHPYGGQDMQVMVNLDQQKLLARNLTPADIHNALMQQYLVLPS
                     GDIKIKQTDWIVQTNASPMQIEHFSDIPVKREDNAFIYLRDVATVRLMGRVQQNAVLV
                     KGKQTVIIVAMKSTEASTLDVVNGIKKMIPRAEQISPEGVEIKLLDDASTFVKDSIAD
                     VLHEMATAGALVGLIVLLLLGSWRATVIVWTSIPLSILTALIGLHWLDETINVMTLGG
                     LALAVGILVDDATVMIENIDRHLEMGKPLEQAIVDAANQIVVPTFVATLCIAIVWIPL
                     FELGGVAGYLFKPMAEAIIIAMLASFILSRTLVPTMAKYMMKGHHVEAA"
     gene            2833..3633
                     /gene="modA"
                     /locus_tag="PLS35_17735"
     CDS             2833..3633
                     /gene="modA"
                     /locus_tag="PLS35_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI20566.1"
                     /translation="MAVGLVGGLAIGEPAEAENLTIGAAYSLKAAFQEVVPMFESEFG
                     ATVNVVYGSSQTLRREVESGAPVDVLLSAVEDLEKLQKKGLTLNGGPEVYAQTSLVFV
                     MSAASQAMPISFHDLLSERRTRVAIGAFESAVAGPITARALRAVDPSYKDRFRLISAT
                     HHDEVIEMIRREEAEMGLVYRVDAINNSGQVRIIDEMPGGTYTPVRFGQAIVWTCRDE
                     SRTAAEQFTDFFMSPRIQKLLLKYGFEPAASHVGENKVARTAPKVRVN"
     gene            complement(3640..>4916)
                     /locus_tag="PLS35_17740"
     CDS             complement(3640..>4916)
                     /locus_tag="PLS35_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNI20567.1"
                     /translation="FDERHRSAKEISEEMNQKISAIKEGITFVLMPPPILGLGNGAGY
                     GLFVEDRAGLGFGALQNAVGGLQGAVMQTPGMGYPISGYQANVPQLDAEVDRVKTKTQ
                     GIALTDLFATMQIYLGSAYVNDFNLFGRTWRVYAQADGDFRGKVEDISNLKTRNARGE
                     MVPIGSMVKFSQTYGPDPVLRYNGYPAADFMGEADPTKFSSAQAMDTIRDIAGKVLPN
                     GMTIEWTDLSFQEASQGKAALLVFPIAILLAFLVLAALYESWTLPLAVILIVPMCMLC
                     ALAGVKLTGGDNNTFVQVGLVVLMGLACKNAILIVEFARELELQGKGIVEAALEACRL
                     RLRPIVMTSVAFIAGTVPLVLSHGAGAEVRSATGITVFAGMIGVTLFGLFLTPVFYVG
                     LRMLSGRKLIRHDEGMDVARPAGQLSGVENPH"
BASE COUNT         1052 a         1362 c         1503 g          999 t
ORIGIN      
        1 tgggatcgag ccaatacacc tggtcgatct gttggctgcc ggcggtcgtc atcaggaggt
       61 tggacgccat ttctttttga gtccggttga cggccagacc gaaggtgcgg ttgccttcga
      121 ccatgagcgt cggcgtccgc atggtctgtt ggatgaccac gtcggtcgcg ccggggatct
      181 ggcgtagtct gccggccagt ttgcgggtga actcatagtt gggatgcatg tccggaccct
      241 tgatctgaac gtcgatcggg gccggtgcgc cgaagttcag gatcttggca gtcaggtcgg
      301 acggctggaa cgtaaactct gtaccagggt aacgctccgt caaccctttg cggagaatgc
      361 gccggtactc ccacaccggc gacttttcat ccctcaacag aatcgtgagg tcgcaatcct
      421 gcgatccgat ggtcggcgtc ggaatgaacg ccaagttatg cgctcccacc ggtaagccac
      481 aattgctcac gatgttttcc acctcaccgg ccagcagctc ttcgacactg ttggagacca
      541 gcgtcgcgat acgtccggat acctcgatgc gtgttccgag cggggcccgc atatgcatct
      601 gaatgacacc ggatctgatt tcgggaaagt agtcgcggcc gagaaagaag aagagtgaca
      661 gcgaggccac ggcgatcacg aggcagaccg tgacaaacgc ccggcgccgg gccatggccc
      721 gttcgagcag cagcccatag cgctcacgga accggttgaa cccgcgttcg aacccttgtt
      781 gaaaccgaac gaagatattg cctgagacaa ccggaccggg ctgttcagca ggttcaagcg
      841 gcttcgacat ggtgcccctt catcatgtat ttggccatgg tcggcactaa ggtgcgcgac
      901 aggatgaagg aagccagcat cgcaatgatg atcgcttccg ccatgggttt gaacaggtag
      961 ccggctactc cgccgagttc gaacagcgga atccagacaa tcgcaatgca gagggtggcg
     1021 acgaatgtcg gcaccacgat ttgattcgcg gcatcgacga tggcctgctc gagcggcttg
     1081 cccatttcga gatgccggtc gatattctcg atcatcacgg tggcgtcatc gacgaggatg
     1141 ccgacggcca gtgccaatcc gcctaaggtc atgacgttga tcgtctcatc gagccagtgc
     1201 aaaccgatga gggcggtgag gatggagaga ggaattgacg tccagacgat caccgtggcc
     1261 cgccatgatc cgagcaacag cagcacaatc aggccgacca gtgcgcctgc ggttgccatt
     1321 tcatgcagca catcggcaat cgaatccttc acgaaggtgg aggcgtcgtc gagcagtttg
     1381 atctcgacgc cctcgggaga gatctgctcc gcgcgcggga tcatcttctt gatcccattg
     1441 accacgtcga gggtcgaggc ctccgtgctc ttcatcgcaa cgatgataac tgtctgtttg
     1501 cctttcacca gtaccgcgtt ctgctgcacc cggcccatga ggcggacagt ggccacatcg
     1561 cgcaaataga tgaaggcgtt gtcttcccgc ttgaccggga tatcgctgaa atgttcgatc
     1621 tgcatcgggg aagcattggt ctggacgatc caatcggttt gcttgatttt gatatcaccg
     1681 gacggcagca ccagatactg ctgcatgagg gcattgtgaa tatcggccgg cgtcagattg
     1741 cgggcaagca gcttttgctg gtcgagattg accatgacct gcatgtcctg accgccatag
     1801 gggtggggga ggacgattcc cggcacggtc acgagtaagg gccggattcg catgtaggcc
     1861 agattgtaga gctccgccgg ggtcagccgg tcggaactga tctgcagcat cgccacaggg
     1921 atggaagagg gcgcgagacg catgaccatg ggtggcgaga tatccggtgg cagcgccttg
     1981 acgacggtct gcgcaatagc cgtgatatcc gcctcggctc tccccacatc cactccgtcc
     2041 tggaggaaaa tgttggtgat gctgctgccg taatacgagt gactgtgaat gtatttgatg
     2101 ccctccaccg tcgaagtgag aaagcgctcg aagagatagg tgatgcgtcc ttcgacctgc
     2161 tgcggcagca ggccggcata tgcccacacc accgaggtga cggggatcgt gatattggga
     2221 aacacgtcgg tcggcatgtg gagcaccgtg atgctaccga gcagcacgat gagaatggac
     2281 atcaccacga aggtgtaggg acggcggagg gcaatgagga cgatttcatt catacagcaa
     2341 gtcctcgtcc tgcaatacgg ggattgcaga tctatgatta gaacacgaag agcagtgtgc
     2401 gattcttgat gacgggatag agcaaggttc ttgccgacct gcgcctgttg tgacgacgga
     2461 cgacaatggt cgtgtattcg cacgttttgg agggagcgat cactgaattt gattgcgtcg
     2521 tcttgcgaca acgggaaggc aaattcttga aggggcatta tcgcactgtg cgcacagtgc
     2581 aaagtctcac ggttttgcca gacgagaaat ctcagcggtc atgcaaaaag ctccgtcatt
     2641 tcacaagaac ctcaacaatt gttccggctt tcggagatcg tgggacgacg gatgcggctg
     2701 gtactaatct tgcgacaatc tcgtcgtgtg cggaagctgg cgtatggcaa tgttcgcgaa
     2761 gacctgcgca gagggaacac gggggaggtt gacgtaatga agacattccg gagattcagc
     2821 aaggcgctcg taatggcagt cgggcttgtg ggaggactgg ccatcggcga gcctgcagag
     2881 gcagaaaatc tgacgatcgg cgcggcctat agtctgaagg cggcctttca ggaagtggtg
     2941 cccatgttcg aaagtgagtt cggggccacc gtcaacgtcg tgtacggttc ctcgcaaacc
     3001 ctccgccgtg aagtcgagag cggagcgccc gtggatgtgt tgctatccgc ggtggaggat
     3061 cttgagaaat tgcagaaaaa gggattgacc ctcaacggcg ggcccgaggt ctatgcgcaa
     3121 acgtcgttgg tgttcgtcat gtcggcggcg tcgcaggcca tgccgatttc ttttcacgac
     3181 ctgttgtctg agcgaagaac cagggttgca atcggggcgt ttgaaagtgc tgtcgccggc
     3241 cctattaccg cacgggctct tcgagcggtt gatccttcct ataaagaccg gttccgtctg
     3301 atctcggcca cgcatcacga cgaagtcatt gagatgatcc gacgggaaga agcagagatg
     3361 gggctggtgt accgcgtaga tgcgataaac aacagcggac aggtgcgcat tatcgatgag
     3421 atgccggggg gtacctatac gccggtccgg ttcgggcagg cgatagtgtg gacctgccgc
     3481 gatgagtcac gcacggctgc cgaacaattc accgatttct tcatgagtcc ccggattcaa
     3541 aagcttctgc tcaaatatgg attcgagcca gcggcatcgc atgtgggcga gaataaggtg
     3601 gcgcgcactg caccaaaagt tcgggtcaat taacccgcct caatgcgggt tctcgactcc
     3661 gctcaactga cctgccggcc gtgccacgtc cattccttca tcgtgcctga tcagcttgcg
     3721 tccggatagc atgcgcaggc cgacgtaaaa cacgggtgtc agaaacaagc cgaacagagt
     3781 cacgccgatc atgccggcga atacggtgat gccggtggcg gaacgcactt ccgccccagc
     3841 gccatgtgac agcacgagtg gaaccgtgcc ggcgatgaag gcgaccgacg tcatcacgat
     3901 agggcgcagc cggaggcggc aggcttcgag ggctgcctca acaatgccct tgccctgtaa
     3961 ttcgagctcg cgcgcgaatt cgacgatcag gatcgcgttc ttgcacgcca gacccattaa
     4021 caccaccagg ccgacctgca caaacgtatt gttgtcgccc ccggtgagtt tgacgccggc
     4081 cagcgcgcac agcatgcaca tcggcacgat caggatcacc gcgagcggca gggtccagga
     4141 ttcatacaga gcggcaagta cgaggaaggc cagcaaaatg gcgatgggaa acaccagcag
     4201 cgccgccttg ccttgcgacg cttcctgaaa gctcaaatcg gtccattcga tggtcatgcc
     4261 gttcggcagc accttcccgg cgatgtcgcg aatcgtgtcc atcgcttgcg ccgaggaaaa
     4321 cttggtggga tcagcctctc ccatgaaatc ggcggcaggg tagccgttgt agcgcagcac
     4381 cggatcagga ccgtacgtct gactgaactt caccatcgac ccgatcggaa ccatctcgcc
     4441 gcgggcattg cgcgttttga gattggaaat gtcttcgacc tttccgcgaa aatcgccgtc
     4501 cgcctgcgca tacacgcgcc aggtgcggcc gaacaggttg aagtcgttca cataggctga
     4561 accgaggtag atctgcatcg tggcgaaaag atccgtcaac gcaatgccct gtgtcttggt
     4621 cttgacgcgg tccacctcgg cgtcgagctg cggcacattg gcctgataac cggagatcgg
     4681 gtagcccata cccggtgtct gcatgacggc tccctgcagg ccgccgaccg cattttgcag
     4741 cgccccgaag cccagtccag cccggtcttc gacgaagagt ccataccccg cgccgttgcc
     4801 gagtccgaga atcggcggcg gcatcaatac gaaggtgatg ccttccttga tggcggagat
     4861 tttttgattc atctcctcgc tgatttcctt ggcgctgcgg tggcgctcgt cgaacg
//
LOCUS       DAOQEW010000250         4908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7064_length_4908_cov_7.385329, whole genome shotgun sequence.
ACCESSION   DAOQEW010000250 DAOQEW010000000
VERSION     DAOQEW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7064_length_4908_cov_7.385329"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            677..922
                     /locus_tag="PLS35_17745"
     CDS             677..922
                     /locus_tag="PLS35_17745"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNI20568.1"
                     /translation="MDTIGAFEAKTHLSALLERVSKGETITITRHGVPAAKLVPVQGD
                     VGRLPHAALLEGMRELRKRVKPGAMPVKDMIEEGRRR"
     gene            928..1359
                     /locus_tag="PLS35_17750"
     CDS             928..1359
                     /locus_tag="PLS35_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNI20569.1"
                     /translation="MVIDASVALAWCFPDEQSPYADKVLQELDGCSVLVPSVWALEIT
                     NGLLVGERRRRIGQSDILRFIDLLDALSPQEVSVPINAHFHALLPLGRGYGLSAYDAA
                     YLHTAIRTEAELATADDALEKAARTAGVPIMCTSHLRKPRR"
     gene            complement(3374..4267)
                     /locus_tag="PLS35_17755"
     CDS             complement(3374..4267)
                     /locus_tag="PLS35_17755"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNI20570.1"
                     /translation="MAAFLQERAPTIADTTVATYARKLSTFHEWWCNHEPSLPFSETL
                     ATRYARWLATHSTRSQASQGLHLSAVRQWACFLLAAGHLSINPLAGVSGPPRARWLRH
                     RLLTRTDLQGLLKQFAVTTLVGQRDYLLAALMIRTGARESELAVANIGDLTEYGEEGS
                     LLLLHSKGKAKQEPVVVRPDLSTKLWAYLRLRYSDGRLPPQDPLFTNHHPSSRGERMT
                     TRGMRKQLKAAFREAGLAEPHVTALSLRLSGGALAYRKGASPSELKKTMRHESIQTTQ
                     VLIDQVNRIRFGAERYLDDIQ"
BASE COUNT         1081 a         1402 c         1358 g         1067 t
ORIGIN      
        1 acaaggacat ccgcaccact caaaagtatt ttcacgcgat tgatcggctg gaggaagggg
       61 cggagtatgc gatcacacgc tactaaagag tctagccatg acactctcaa cttcggtgcg
      121 gatcaatatg gagtttttga cacgcgttgg gctctcacag actgcgatca cataaataca
      181 taacgtctgc cgtgaataaa catttcatcc acgctggagt ccaatagact ttaaggtatc
      241 ccaagtgggc gggcgttccc taggttcccg acattccagg cgtggagact cggacaccaa
      301 atcctaccac gaccatcctc gacaagcgcg tcccacgacc tggaagagag gcggagtatg
      361 aagcaccggc cgtcctcctg cccgggctgc gggcgctcta ccgcaagcgc aatcactgct
      421 ccgacgcaga ggttgatggc gttgatgaag cgattctccg actgttgagt tcggggaatc
      481 gttcctgaat actcgacgac ggcgcgaaaa ctcccaaacg gagtcgaggt ttaaacggct
      541 tctcgcggag ggtggcttgg tcgtacatcg agcaaccagg caccatcccc taaatttcca
      601 atggaagcct tgactcgaca gacgagagac gctaaacttc aactagttgt agactagatc
      661 aattggaggc aggcacatgg atactatcgg agcatttgag gcgaaaacgc atttatctgc
      721 cctgcttgaa cgggtgagta agggagagac gattaccatc acgaggcacg gcgtgcctgc
      781 tgcgaagttg gttcccgtac agggagatgt cgggcgcctg cctcatgcgg ctcttctcga
      841 gggcatgcgc gagttgcgca agcgggtgaa gccaggggcg atgcccgtca aagacatgat
      901 cgaggagggc cgtcggcgtt gaggcgcgtg gtgatcgatg cctcggtggc gctggcatgg
      961 tgcttcccgg atgagcaaag tccttatgcc gacaaagtcc tccaggagct ggacgggtgt
     1021 tctgttctcg tgccgtctgt atgggcgctc gagatcacca acgggctgct tgtcggtgaa
     1081 cgacgccgac gcatcggcca atccgacatt ttacggttca tcgatctctt ggacgcgttg
     1141 tcaccacagg aagtctcagt cccgataaac gcgcattttc acgctcttct gccgcttgga
     1201 agaggatacg gcctttccgc gtatgatgcg gcctatcttc atacagcgat aaggactgaa
     1261 gctgaactgg ccacggccga cgacgcgctg gagaaagcgg cgcgtacggc tggggttccg
     1321 atcatgtgca catcacatct ccgtaaaccc agacgataac ttgaaatgcc tagttgatcg
     1381 ggtaagacgt aggaggatgg cctgatgttg tgttgtgact tctgttcggc ttcaatcccc
     1441 accaccaaat atcggaccca cccattcgga ctgaccgact caagtcaggt ctttggcgat
     1501 acgccgattt atactattca agggctcgac catgtctcca ctgaggattt acatccgtcg
     1561 catggcacgg gtgccatgat gagtcctgaa tggctcgcct gtgacgactg cgccatcttc
     1621 attgacgcga gggatagaga agggcttgtg caatactgtg tgatgcgcct ctcagcacaa
     1681 cccaacctgg aatcaattga catcgcgaat gagctgattc ccatgtatcg aaagctgttc
     1741 accgctttct tcctttacct tgaatcaaaa gaaccgtctg ccgcgtcccg atcagacctt
     1801 acctgacgct gcctgataca tgcactttcc ccccttcatc ccatacctcg gccgtgacca
     1861 aaaccgatgc tgtactcatg aacgagcgaa tcccacctct gtaacgctac tctcgaggtg
     1921 gattttttcc cgcaccgtct ggctgccgca ttatgacgga gcgcatcttt aatgcgaccg
     1981 tggcgctcgt agagaccggc taggcagcag tgcgcccgct caaactcacc gtcatgcccg
     2041 agcgcctact cataggcatg catcgccgcc tggtccttgc cttggtcttc aagtaccaca
     2101 cctaaattga agatgcgacg gctccatctg gatcggagtg aatcggcgcg cgatggctcg
     2161 cctccgcctc tgcctgtttt tccgcatcat gacacagccg tccgagattc acacgtgctt
     2221 cggcgaatga cagatccagc ggaatggctt cttgatacgt ctgacaggcc gttctggcac
     2281 atgtccttca agctccacgc cacgattcca tcagaccaac gccgatcttt tgtctagccg
     2341 actggttcgg cctctcgatg ggagccgggc cgttctggcc ggcagctgca cctcgaaatt
     2401 cagcacatag tgaccagagt ccggttgcca tcggtctcgg ccatcccaca cgatcactcc
     2461 acgccttgac gcatagactt tgacactagc catggagcga gtctccaaac tgccgctgca
     2521 ggaaagcgag cacggtccgt atccgtcgca acgaaacccg acgtccttca gaccattcat
     2581 agtccgtaac cacctgctgc tcgaacgaat cggatggcat atgtgttttg aggggcgaca
     2641 cggtgagaag ggcggcgcgc aggtaggtcc gcaattcggc gttgctaatg tgcaaaatac
     2701 tacacagcgc ggaggaagcg tagtacccgt catattctga aatatcttgc tgaccctccg
     2761 catggaatgc agtcacaagg attaaccagt ggggattcca tgcgattgat cacgacgaag
     2821 gaactgagaa acatgcacat tgccacacgg catcatcatc tcatcaacag ccggctggcg
     2881 atgttgcgct atgccgacga atatgggttc aagggcgccg cccgacggtt tagcttgaat
     2941 cgtaagacgg tgaggacctg gcaccgacgc tgggtagtca gtggaccggc ggggttggtg
     3001 ccacgccatc cgcgcacacg cgccgcaagc tgcactttgc cgcgatgcgg acccggttct
     3061 tgctggatcg ggtgcatcgc atctgcgtag cggccgcgac atcccccgaa tctcccggaa
     3121 cctcggctat ccgcccattc ggcgcaccgg cccgcgacac cctgaacaga aggggagcat
     3181 cagtacaacc acgaccgctt ctctatggcc ttccagggcc tgacaccagc gaagaaattg
     3241 gcgacatttc tctcgccacc ggcattatca tcaccaacca cgccatgcgg acatacaggg
     3301 gccgcggtgt gaccagccat tgcaacccag gaccctcgcc ttgaaagata ccctctcatt
     3361 cccatcttgt tgtctactga atatcgtcca gatagcgttc cgctccaaat cgtatgcggt
     3421 tgacctggtc aatgaggact tgggtggtct gaatggattc atgccgcatg gtcttcttga
     3481 gctcagatgg gctcgcccct ttccgatagg ccaaggctcc gcctgacagc cggagtgaca
     3541 gggcggtgac atggggctca gcaaggccag cttccctaaa agcggccttt agctgcttgc
     3601 gcattccccg tgtcgtcatg cgttcccctc gggaagacgg atgatgattc gtgaacagcg
     3661 ggtcttgtgg cggaaggcgg ccgtcagaat atcgcaggcg gagataagcc caaagcttcg
     3721 tactgagatc cggtcgtacc acgacgggtt cctgtttggc tttgcctttg ctgtgcaaca
     3781 acagcagact tccttcctct ccgtactccg tgagatcacc gatattggcg acggccagtt
     3841 cggactcacg ggctcccgtg cgaatcatca acgcagcgag gagatagtcc cgttggccca
     3901 cgagcgtggt caccgcgaac tgcttcaaca atccttggag atccgtacgg gtgagcaggc
     3961 gatgccgcaa ccaccgcgct cgcggtggcc ctgacacacc cgcaagggga ttgatagaca
     4021 ggtggccggc ggccaggaga aaacaggccc attgccggac ggccgacaga tgcaggcctt
     4081 gtgaggcttg gctccgtgtg ctgtgcgttg ctagccagcg cgcgtaccgt gtcgccaacg
     4141 tttcggagaa gggaagggat ggttcatggt tgcaccacca ttcgtgaaac gtgctgagct
     4201 ttcgggcata ggttgcgacg gtggtatcgg caatcgtcgg cgctcgctcc tgtagaaatg
     4261 ccgccattag agctgtggcc tggtcggatg tgaccaatgg tctggctgat tccatagtac
     4321 ctggtatacc aagagtgtac ccatcctagc aacactcttg gggcgcgcgt gtcagaatgt
     4381 ctgacaaaaa agcttctgaa ctcttggttt gtgtggcgag gtaacgctta gtataccagg
     4441 cacctatacc ccacgtaccc aaatgctgcg ttggggcgcg cgtgatgtga cattgaagtc
     4501 ctaccttgtt ataagttttg atacggcaac tacattttac cttagaccga gatcttcttc
     4561 ccgttcttga tgagccaagt aaggtgaaat cgcagtaggg gaaaccctga catctctagc
     4621 ctgagaaagg tgaaagtgct tggagtagcc accacagtga ccggctgcgg gtttcaaaga
     4681 gaccgagttt agagtcacca attgaaaatt cccgaaggca gatcccaagc gatcctcggt
     4741 tcgactcgtc cgattccgca aagttcggcg agttgcgtcg accgacaggc ctctttctca
     4801 tgcgcatcga tgcgattcca gtggagggtg gcggtgccag gattatactg ttttcgagat
     4861 ggcgatcacg acaggcccaa ccgatctgtc accgaatcca cgagatga
//
LOCUS       DAOQEW010000251         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7073_length_4905_cov_10.356289, whole genome shotgun sequence.
ACCESSION   DAOQEW010000251 DAOQEW010000000
VERSION     DAOQEW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_4905_cov_10.356289"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLS35_17760"
     CDS             complement(<1..499)
                     /locus_tag="PLS35_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20571.1"
                     /translation="MAKEDQQDNSAKPKSDSYSADQIKVLEGLDAVRKRPAMYIGSTG
                     VDGLHHLVYEVVDNSVDEHMAGFGEAIEVTIHIDGSVTVIDNGRGIPTGMHSTQKKSA
                     AEVALTVLHAGGKFEQGAYTVSGGLHGVGISVVNALSEWLELEIWQDGQVFEQRYERG
                     KPQASL"
     gene            complement(653..1783)
                     /gene="dnaN"
                     /locus_tag="PLS35_17765"
     CDS             complement(653..1783)
                     /gene="dnaN"
                     /locus_tag="PLS35_17765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNI20572.1"
                     /translation="MKVRIGREELLTGLQRVQGVVEKRNTMPILSNILLEAKHDGAEI
                     VATDLEIGMRGLYKATVLEAGGVTISARKLYEIIKELPSGEIELTSGENNWTTIQFGK
                     SQFKVVGLPSSDYPALPSIEREGLTPLAGAGLLELIRKTLFAAGDNDARYILNGLLVS
                     LTTTERKTTLLRLVGTDGHRLAVAEREVGSPNAKPLAQDIKAIIPKKAAQEMRRLLEE
                     GGDEEPLIGFTKNLMIFRKSGLLLTSRLMEGNYPNYQQVVPKESGKRIVVNRGLLESA
                     LRRVSVLSKDKANAVKVSFAAGGMTLFSSNPDYGEATEELVARYEGEALHTGFNARYL
                     LDALSVMDGESVSLQMDTALSPCLIQEAESPGFKCVVMPIKI"
     gene            complement(1807..>2091)
                     /locus_tag="PLS35_17770"
     CDS             complement(1807..>2091)
                     /locus_tag="PLS35_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI20573.1"
                     /translation="SIEDIQEAVGSKYHVKIADLKSRRRSKTLVHPRQIAMYLCRELT
                     DASFPEIGRQFGGKDHTTIIHACRQITKAKEADSALHTTLEGLKEQILRA"
     assembly_gap    2092..2191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2192..2982)
                     /locus_tag="PLS35_17775"
     CDS             complement(<2192..2982)
                     /locus_tag="PLS35_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNI20574.1"
                     /translation="MNQMWNEALEYIQEKVPKQVYETWFTPVVLDRIEETTAYLAVPN
                     KFFGDWLGEHYHDLLAEAVSAAQGGGRMDVSFVVSQKTAQAQAQPDHSAADTAGRGFM
                     ASRSKRGIQLNPKYTFKSFVVGAGNQFAHAACMAVAEQPAKAYNPLFLYGGVGLGKTH
                     LLNAIGNYLAERSDLRIAYLTTEQFTNEVINSIRYDKMIDLRKRYRNVDMLMIDDIQF
                     LAGKERTQEEFFHTFNTLYEAHKQIVLSSDRFPKDMPDIEERLRSR"
     gene            complement(3331..3603)
                     /locus_tag="PLS35_17780"
     CDS             complement(3331..3603)
                     /locus_tag="PLS35_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20575.1"
                     /translation="MSEHKTLYLLRQPISDPAETLLPSAAETQLADRVSLVLLEAAAS
                     TTPSFPGPIYVLHQDSATPVQGGSRTQISYRELVTLIAEHPSTIVL"
     gene            complement(3618..3968)
                     /locus_tag="PLS35_17785"
     CDS             complement(3618..3968)
                     /locus_tag="PLS35_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20576.1"
                     /translation="MAATATIALIIRENPLTSAVPVEALRIALGLAAGENPITVILMG
                     PAVHLLAEESDDIVDLEILEKYLPSFEHLQIPFILVHSSGPRPDLQEGFAVTDGTVES
                     ARQAMAAADRVLVF"
     gene            complement(3989..4309)
                     /locus_tag="PLS35_17790"
     CDS             complement(3989..4309)
                     /locus_tag="PLS35_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNI20577.1"
                     /translation="MGALKIGLLLSTPPSHPSVQTVASLASEAISQGADVYLYCIDEG
                     VKNLRDPRYTELVGRGMKLFVCAYGCQQHGVSTDQLDPRISLCGLVVLSNIVNGCDRF
                     LAFT"
     gene            complement(4429..>4905)
                     /locus_tag="PLS35_17795"
     CDS             complement(4429..>4905)
                     /locus_tag="PLS35_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNI20578.1"
                     /translation="YCLTAFEVTCSHLGAQNAVGAGGRYDGLVRELGGPTVPAVGFAL
                     GLERIALMLPETFVVPAIPRVYVAAFGTQAADAGFVLLDELRRAGVPADMDFRSASLK
                     AHLRQADRLGALYTILLGDDEVAKGTATVRNMQTKAQEDLPIQDLATAFRNRLLNK"
BASE COUNT         1015 a         1408 c         1308 g         1074 t
ORIGIN      
        1 ccagcgacgc ctggggtttc ccgcgttcat aacgctgttc gaatacctgg ccgtcctgcc
       61 agatttccaa ctccagccat tcggacaggg cgttgacgac ggagatgccg acaccgtgca
      121 acccgccgga caccgtgtag gcgccctgct cgaatttgcc gccggcgtgc aggacggtga
      181 gcgccacttc tgcagcggat ttcttttgcg tggagtgcat cccggtggga ataccgcgtc
      241 cgttatcgat gaccgtcacg ctgccgtcga tatggatcgt gacttcaatg gcttccccga
      301 agccggccat gtgttcgtcg acgctgttgt cgaccacttc gtagacgagg tgatgcaacc
      361 cgtccacgcc cgtgctgccg atgtacatgg caggtcgttt gcgaacggca tcgaggcctt
      421 cgagtacttt gatttgatcc gcgctgtaac tgtcggattt gggcttggcg gaattgtcct
      481 gctggtcttc cttggccatt cagctcctag caggatactg accacgtccg ccggcggcgt
      541 tctcgcatcg cggaggccct caacgtaccg caagggtacg cctcggccct ccactcgcta
      601 cggccttgcc ggacgattgt gttgagtatc ctgaaacagg tcatgtttga tgctagatct
      661 tgatcggcat cacgacgcat ttgaatcccg ggctctcggc ctcctgaatc aagcaggggc
      721 tcaaggccgt gtccatctgc aacgaaaccg attctccgtc catgacgctc agagcgtcca
      781 aaaggtatcg ggcattaaag ccggtatgaa gggcttcacc ctcgtagcga gccaccaatt
      841 cttccgtggc ttcaccataa tccggattac tggagaagag cgtcatgccg cccgcggcga
      901 aagacacctt aacggcattg gccttgtcct tcgacaacac cgacacacgg cgcaaggcgc
      961 tctccaacaa tcctcgattg accacaatgc gcttaccgct ctccttcggg accacctgct
     1021 ggtagttggg ataattccct tccatcaggc gggacgtcag gagcaggccg ctcttgcgga
     1081 agatcatgag gtttttcgtg aagccgatca gcggttcttc gtcgccgccc tcttcgagca
     1141 ggcgccgcat ttcctgagcc gcctttttgg ggatgatcgc cttgatgtcc tgcgccaacg
     1201 gcttcgcatt cggactcccg acctctcgct ccgccaccgc caggcgatga ccgtcggtgc
     1261 cgacgaggcg caacaacgtc gtcttccttt cggtggtcgt caaactgacg agcaggccgt
     1321 tgaggatgta gcgagcgtcg ttgtcaccgg cggcgaacag ggtcttgcga atcaactcca
     1381 gcaggcctgc ccctgccagc ggcgtcagtc cctcccgttc gatcgagggc aacgcgggat
     1441 aatcgctgct cgggaggccg acgactttga attggctctt gccgaattgg atggtggtcc
     1501 aattgttctc gcccgaggtg agctcgatct cgccgctcgg cagttccttg atgatctcgt
     1561 acagcttgcg cgccgaaatg gtgacgccgc cggcttccaa caccgtggcc ttatacaacc
     1621 cccgcatccc gatttcgaga tccgtcgcga cgatttccgc cccgtcgtgt ttggcctcca
     1681 gcaggatatt tgagaggatc ggcatggtgt tgcgtttctc cacgacaccc tgcacgcgct
     1741 gcaggcccgt caacaattcc tctcgtccga tgcgtacctt catggtcggt ctccctcaga
     1801 gggccttcac gccctcaaga tctgttcttt caagccttcg agcgtcgtgt gcagcgcgct
     1861 gtcggcctct ttggctttcg tgatctgccg gcaggcgtga atgatcgtgg tgtgatcttt
     1921 ccctccgaac tggcggccga tctcaggaaa cgaggcgtcg gtcagttccc gacagagata
     1981 catggcgatc tggcggggat gcaccagcgt tttgctccgc cgacgcgatt tcagatcggc
     2041 aatcttcaca tggtatttcg atcccaccgc ttcctggatg tcttcgatgg annnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgaccga agccgctctt caatatcggg
     2221 catgtctttc ggaaagcggt cacttgagag gacgatttgt ttatgcgctt catacagcgt
     2281 attgaaggtg tggaagaatt cttcctgcgt ccgttccttg cccgccaaaa actgaatgtc
     2341 gtcgatcatc aacatatcga cattgcggta gcgcttccgg aggtcgatca tcttgtcgta
     2401 gcgaatcgag ttgatgacct cgttcgtaaa ctgttccgtc gttaaatagg ctatccgcag
     2461 atcgctgcgc tcagccagat agtttccaat ggcattcaac aggtgagtct tccccaatcc
     2521 caccccgcca tacagaaata acgggttgta cgctttcgcc ggttgctcgg ccacggccat
     2581 gcaggctgcg tgggcaaact ggtttccggc gccgaccacg aaacttttga aggtatattt
     2641 gggattgagc tgaatgccgc gcttggagcg cgaggccatg aatcctcgtc ccgccgtatc
     2701 cgcggccgag tgatctggct gcgcctgagc ttgtgccgtc ttctgactga cgacgaacga
     2761 aacgtccata cgcccgccac cctgggccgc cgataccgct tccgcgagca gatcgtgata
     2821 atgctccccg agccaatccc cgaaaaactt gttcggaacg gcgagatagg cggtcgtctc
     2881 ttcaatccgg tccagcacca ccggggtgaa ccaagtttca tagacctgtt tcggcacctt
     2941 ctcctgaatg tactccaacg cctcattcca catctgattc atctttttat ttcaatcact
     3001 tacaaatgtt cacaggctta tacacaggtg tggacaaata cataggaaca cgcgattggc
     3061 ttgatgcggc gctgggaatc tttccagggt tcctgtggac atcgaatcat tccgacaatc
     3121 atcgcgatcc ctcgtgcaca ggcctcctca cgttatccct gaaggccccc tgccttatac
     3181 accatctcat caacatgggc caggatcttc catcgccaat gcccgcgcag tccttcacgc
     3241 tcatccacag aacccacacg tcctactcct actccgacgg ctccggatct tctctttata
     3301 aagaacatat ataagagaag agcatccaga tcacagcaca atggtcgaag gatgctcagc
     3361 aatcaaggtg accagctctc gataggaaat ctgtgttcgg gaaccacctt gaaccggtgt
     3421 agcggagtcc tgatggagaa cgtagatcgg gccgggaaag gaaggggtcg tcgatgcggc
     3481 agcttccaga agtacgagag agacccggtc agccagttga gtctcggccg cagacggtaa
     3541 cagcgtctcg gccggatctg aaatcggctg gcgaagaaga tacagggtct tatgctcaga
     3601 catacaactc agccttccta aaacaccaag acccggtcag cagcagccat ggcctgccgg
     3661 gcggattcta ctgtcccgtc agtgacggca aatccttcct gaaggtcggg ccgcggcccg
     3721 gaggaatgga cgagaataaa gggaatctgg agatgctcga aggaggggag atatttttcg
     3781 aggatctcca gatcgacaat gtcgtctgat tcttcagcca ggagatgaac cgcaggtccc
     3841 atgagaatga cggtgattgg attttcaccc gccgccaacc ctaaggcaat gcgaagtgct
     3901 tcgaccggaa cagcggaggt caaaggattt tcccgaataa tcaaagcgat ggtagcggtc
     3961 gcggccacaa ttcaatccct caggcacatt aggtgaaggc cagaaatcgg tcgcagccat
     4021 tcacaatatt cgaaagcacg actaacccac agagtgagat gcggggatcg agttgatccg
     4081 tcgagactcc atgctgctga cacccgtagg cacagacgaa caacttcatt ccgcgcccga
     4141 cgagttctgt atatcggggg tcccgaagat tcttcacccc ttcatcgatg caatacagat
     4201 acacgtcggc cccctggctg atcgcttcgg acgctagtga ggcgaccgtc tgtacactgg
     4261 ggtgtgacgg gggtgtggat aacaggagac cgatcttcag cgcgcccata aaaaatgtgc
     4321 ctgtggaaaa actttaggag ttcttaataa tcaattactt atatgtttta tactgcacgc
     4381 ggagcgtacg aattttgggc cggaaagtca actagaaact gagcgggatt acttgttgag
     4441 aagtcggttt cgaaatgccg ttgcgaggtc ttggatagga agatcttcct gtgctttcgt
     4501 ctgcatattg cggacggtcg cggttccttt tgcgacttca tcatcaccca ggagaatggt
     4561 atagagcgct ccgaggcgat cggcctgcct gagatgagcc ttgagggatg cggagcgaaa
     4621 atccatatcc gccgggacac cggcccgacg aagttcatcg agcaggacaa acccggcatc
     4681 tgcggcctgt gtaccgaagg ccgcgacata gactctcggg atggcgggta cgacaaaggt
     4741 ctcgggcagc atcaacgcga tccgttcaag accgagggcg aaccccacgg cgggaacagt
     4801 tggccctccc agttctctga ccaatccatc ataccgtccg ccggcaccca ctgcgttttg
     4861 cgcccctaag tgcgagcagg tcacctcgaa agccgtgagg caata
//
LOCUS       DAOQEW010000252         4865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7178_length_4865_cov_8.894387, whole genome shotgun sequence.
ACCESSION   DAOQEW010000252 DAOQEW010000000
VERSION     DAOQEW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7178_length_4865_cov_8.894387"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..1286)
                     /gene="glyA"
                     /locus_tag="PLS35_17800"
     CDS             complement(9..1286)
                     /gene="glyA"
                     /locus_tag="PLS35_17800"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNI20579.1"
                     /translation="MQDLIGSLDALKSTDPETYEAIVAEEQRQRDKLLLIASENFASP
                     AVLSAQGSVMTNKYAEGYPGKRYYGGCQHVDTVESLAIDRAKQIFGAEHVNVQPHSGS
                     QANMAAYLAVLKPGDTILGLDLAQGGHLTHGSKVNFSGIIFRAFSYGVDRQTETIDYA
                     AVRKIAEECRPRMLVVGASAYARTLDFAKFQDIAKSVGAYLLVDIAHIAGLIAAGLHP
                     NPVPYADFVTTTTHKTLRGPRGGVTMCKAEHAKAVDKIIFPGLQGGPLMHVIAAKAVA
                     FKEAMSPAFKRYQQQVLANARTLAQGLVDRGYKIVSGGTDTHLMLVNLTNKGITGKEA
                     DAALDAAGIIVNKNAIPYDEKPPAVASGIRIGSPIVSTRGMREAEMREIVALIDRVLQ
                     HPQDSQVQAEVRAQAKTLCNRFPIFHAYDSSPA"
     gene            complement(1341..1826)
                     /gene="rplI"
                     /locus_tag="PLS35_17805"
     CDS             complement(1341..1826)
                     /gene="rplI"
                     /locus_tag="PLS35_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNI20580.1"
                     /translation="MKVILQETLEGVGDLGDLLDVSNGFARNYLLPRKKAVEANSRNI
                     KEFEHAKRAAAEKAKKEKQEIEAHGKKISTVSLTIAAQVGKDDKMFGSVTAKDIAEGL
                     AEQGFTVDRRKIQLAQPIKELGTFAIAIKLPREVTATIAVHVVKKQEETEAAEEAAPT
                     A"
     gene            2114..3589
                     /locus_tag="PLS35_17810"
     CDS             2114..3589
                     /locus_tag="PLS35_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20581.1"
                     /translation="MTAMPGTLPDLLDQLAAALERNAAQSVDQGRDQAVALVRGRLLQ
                     TIGTLHLYEFFLPPEVRISADLAASLLLGEETEPTEGLVLFQEGERLVLQVFDAIGEV
                     VPHATLVPDSSGLALTISRRLAEMSKAGTSYTLGPAERLLPIIEAGQSGDRATLAAVT
                     PTSVLTPLWQEDEAARRQKVATLVVELIRANKRILLVTPDHQSADLITLVVARVMKAA
                     GLTFKSWLSRYEVATSKETGGIPLPDLGFEAQMHQFYAKSRSDKAALRKKYDRFRELT
                     PILAYKAQKQKDLDEVRLLEWRLVTQLSDYQAKIKDIDATLAQYEQLPIWKRLSMQAM
                     GKNVESLREYKVIYERYSAGLRKEVDIAKQRIEELIPEATVPKELKPEFAELKEDVIH
                     LGGTKKIRELLAAEENTNRQAFVQNRRLIITTAARVAADPLFQKVRFDVLIAEEAPRI
                     PSPFLLAAAGLVRERIVLSGDLREVVPHGAWSLEDGFSRIV"
     gene            3678..3764
                     /locus_tag="PLS35_17815"
     tRNA            3678..3764
                     /locus_tag="PLS35_17815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3712..3714,aa:Leu,seq:cag)
     gene            complement(4172..4462)
                     /locus_tag="PLS35_17820"
     CDS             complement(4172..4462)
                     /locus_tag="PLS35_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20582.1"
                     /translation="MSIEEDKIEAIRDYLLDALPGYDVRDGSRTHVDRTLTVFHDMEI
                     VCTIQIPTVLLDEDHPSVLELERALQRNNVAAWVLSAPVVYLHHNTLGLPTQ"
     gene            complement(4650..>4865)
                     /locus_tag="PLS35_17825"
     CDS             complement(4650..>4865)
                     /locus_tag="PLS35_17825"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNI20583.1"
                     /translation="PATSTVIRDRHDTIDRYIMEMLHNYPIQDLRTPGRSVTLRDDLK
                     QTVNKVLPKGHVRNVYITSWLITPIGY"
BASE COUNT          953 a         1364 c         1417 g         1131 t
ORIGIN      
        1 cgtcctgcct aagcgggaga cgaatcgtag gcatgaaaga tggggaaacg gttgcacaat
       61 gttttggcct gcgcccgtac ctcggcctga acttggctgt cctggggatg ctgcaataca
      121 cgatcgatca acgccacgat ttcgcgcatc tccgcttcgc gcatgccgcg tgtcgacaca
      181 atcgggctgc cgatgcgaat gccgctcgcc acggccggcg gcttttcatc atacgggatg
      241 gcgtttttgt tgacgatgat gcctgctgcg tccagggcag cgtcggcttc ttttcccgtg
      301 atgcccttgt tggtcaggtt caccaacatc aaatgcgtat cggttcctcc ggacacaatc
      361 ttgtagccac gatcgaccag cccttgcgcc agggtgcggg cattggccag cacttgctgt
      421 tgataccgct tgaaggccgg agacatcgcc tccttgaagg cgacggcttt tgcggcaatc
      481 acatgcatga gcggaccccc ttgcaggccc gggaaaatga tcttatcgac ggccttcgca
      541 tgctcggctt tgcacatcgt cacgccgcct cgagggccac gtaacgtctt atgggtcgtc
      601 gtggtgacga agtcggcata ggggacgggg ttgggatgca acccggccgc gatcaacccg
      661 gcgatgtggg cgatatccac cagcagatag gcgccgaccg atttagcgat gtcctggaac
      721 ttcgcaaagt cgagcgtgcg ggcgtaggcg ctggcaccga cgaccaacat gcgcgggcgg
      781 cactcttcgg cgatcttgcg gactgctgcg tagtcaatgg tttctgtctg gcgatcgacg
      841 ccataggaaa aggcgcggaa aatgattccg gagaagttga ccttgctgcc gtgtgtgagg
      901 tgtccaccct gggccagatc cagtccgagg atcgtgtcgc cgggtttgag taccgcgaga
      961 taggctgcca tgttggcctg cgatcccgag tgcggttgca cgttgacgtg ttcggctccg
     1021 aagatttgtt tggcgcggtc gatggcgagg ctttcgacgg tatcgacatg ctggcatccg
     1081 ccataatagc gtttgccggg gtacccttcg gcgtacttat tcgtcatcac gctgccctgg
     1141 gcggacagga cggcggggct ggcaaagttc tccgatgcga tcagcagcaa cttatcgcgc
     1201 tgccgctgct cttcggcgac gatggcctcg tacgtttccg ggtcggtcga tttcaacgca
     1261 tccaacgaac cgatcaaatc ctgcatgctt cctccgggcg cggcctctga ttcagcggtg
     1321 gccacgcatg atcgcgcgaa ctaggccgtg ggcgcggctt cttcggcagc ttccgtctct
     1381 tcctgtttct tgaccacatg gaccgcaatc gtggcggtca cttcgcgcgg caacttgatc
     1441 gcaatggcga acgttcccag ttccttgatc ggctgggcca attggatctt gcgacggtcg
     1501 acggtaaatc cctgttcggc cagaccttcc gcgatatcct tggccgtcac ggacccgaac
     1561 attttgtcat ccttgccgac ctgtgcggca atggtcagcg acacggttga gatcttcttg
     1621 ccatgggctt cgatctcttg cttttctttc ttggcttttt cagcggccgc gcgcttggca
     1681 tgttcgaatt ctttgatatt ccggctgttc gcctcaacgg ccttcttgcg cggtaataga
     1741 tagttccgag caaacccatt ggagacgtct aacagatcgc cgaggtcgcc cacgccctcg
     1801 agtgtttcct ggagaatcac cttcatactg caactccttc tgttggaaag ggaaaaagca
     1861 tactggcggg ctgtgcaaaa gtcaattgat ccggagcggc cgagaggggt tttcttcgtg
     1921 gtctgggaat gggaaggcct gtgcctgctc gccatggagt accgctagtt atgcgggttt
     1981 tgaagatttc gaacagggga acgtctgatc gctgctggac agggtctttc ccgagtcttt
     2041 aagatacggt ccctgtttca tggcccagcc tgtcacggat gcgccccaac gaaatgcagg
     2101 agcacgaccc atcatgaccg ccatgcccgg cacgttgccg gatcttcttg atcaactggc
     2161 tgccgcgctc gaacgtaacg ccgcacagag tgtggatcag gggcgcgatc aagccgtggc
     2221 gctcgtacgt ggacggttac ttcagacgat cgggaccctg catctgtatg aatttttcct
     2281 tccgcctgag gtacggatca gtgccgatct cgctgccagt ctgttgctgg gtgaggagac
     2341 ggaaccgacg gaaggtctcg tcttgttcca ggaaggcgaa cgtctggtac tccaggtctt
     2401 tgatgcaatc ggcgaagtgg tcccgcatgc cacgctggtg ccggactcca gcgggttggc
     2461 tctcacgatc agtcgtcgat tggctgaaat gagtaaggca ggcacttcgt atacgttggg
     2521 tccagcagaa cgtctccttc ccattatcga ggcgggccaa tccggggacc gggccacgtt
     2581 ggcagctgtg accccgacct cggtcttgac acccctttgg caggaagatg aggccgcgcg
     2641 ccgacaaaag gtggcgacgc tggtcgtgga gttgattcgt gccaacaagc gcatcctgct
     2701 cgtcaccccg gatcaccaga gcgccgatct gatcaccctt gtggtcgcgc gggtcatgaa
     2761 ggccgccgga ctgaccttca agagctggtt gagccgatac gaagtggcga ccagtaagga
     2821 gaccggcggt atccccctgc cggatctggg atttgaggcg caaatgcatc agttctatgc
     2881 caaatcccgg tcggacaagg cggcgctccg taagaagtac gatcgatttc gcgaactcac
     2941 cccgatcctc gcctataagg cccaaaagca aaaagacttg gatgaagtgc gcctcctgga
     3001 atggcgcctg gtcacgcagt tgagcgacta ccaggcgaag atcaaagaca tcgacgccac
     3061 gctggcgcaa tatgaacagc tgccgatttg gaaacgtctg tccatgcagg cgatgggaaa
     3121 aaatgtcgaa tcgttgcgcg aatacaaagt catctatgag cggtacagtg ccggccttcg
     3181 caaagaagtc gatatcgcca agcagcggat cgaggagttg attcccgagg cgacggttcc
     3241 caaggagctc aagccggaat ttgccgaact caaagaagac gtcattcatt tagggggcac
     3301 gaaaaagatc cgcgagctat tggcagccga agaaaatacg aaccggcagg cgttcgtgca
     3361 gaaccggcgg ttgatcatta cgacggcggc gcgagtggcg gcggacccct tatttcaaaa
     3421 ggtacggttc gacgtattga ttgccgaaga ggctcctcgt atcccgagcc cctttctgtt
     3481 ggccgcggcg ggactggtgc gcgaacgcat tgtgctcagc ggcgatctgc gggaagtggt
     3541 cccgcatggg gcctggagtc ttgaggacgg cttctctcgc atcgtgtgat catacccggc
     3601 ccggcggtgc agattttccc ctcactcacc ttgacgggtt gggatcgatc agcgataatc
     3661 cctctcctat tcttcatgcc gaagtggcgg aactggcaga cgcactggtt tcaggtacca
     3721 gcgcccgtaa gggtatccgg gttcaactcc cggcttcggc accacctttc cttctctcac
     3781 cgattcatta gccaaactcc tccgcgctac ttcacgccgc gcgggctttg ttatcctgtc
     3841 tcagtgcctc taatgtgcct cgccataggc tgaggttttc ttaagtcgtc cctcttgaac
     3901 tgaactcctc aagcgcgtag atgtgttgtt acatcacatg gacatttcat ctctcatcgt
     3961 tctagtggtt ggcgtggttc tgctgtttta ttgcgtacgc tttctgataa agctcagcct
     4021 ctcgtttgct cttctcctgc tcgttctcac agtcggcagc gtactgtcgt ttatccttct
     4081 gcccggtttc cgatctatag tcagctcgtt tagaggcgtg tagcctacag cccgtccagt
     4141 ctttacgccc atcagcttga acgctcctgt cttactgggt tgggagacct aaggtattgt
     4201 gatgaaggta gaccacggga gccgacaaga cccaggcggc aacattgttc cgctgtagcg
     4261 cccgttctaa ctcgaggaca gaagggtgat cttcatccag taagaccgta ggaatctgaa
     4321 tggtgcagac aatttccatg tcgtgaaaca ccgttaacgt gcgatcgaca tgtgtgcggg
     4381 agccgtctcg cacatcgtag ccgggcagcg cgtccagcaa gtagtctcgg atcgcttcaa
     4441 tcttatcttc ctcaatactc atgattcagt ccctctcggt cttgcgaaca tgacgccgca
     4501 tcctgcgaat cggtcaacag tgtacgcggg cacatcaagc catgtcatgg cgcctgtgcc
     4561 tacacaccac gtaccggaac tgcgggacat tgttaagcta gggagagccc tggcatcagt
     4621 gctggaaaag gggagcggag aaggaagggc tagtacccaa tcggtgtaat caaccagctg
     4681 gtgatataca cgttgcggac atgtccttta ggcaggactt tattgaccgt ctgcttgaga
     4741 tcgtctcgta atgtcactga gcggccgggc gttcgcaaat cttgaatcgg ataattgtgc
     4801 agcatctcca tgatgtatcg atcgatcgtg tcgtggcggt ctctgatgac ggtgctggtt
     4861 gccgg
//
LOCUS       DAOQEW010000253         4855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7201_length_4855_cov_8.194375, whole genome shotgun sequence.
ACCESSION   DAOQEW010000253 DAOQEW010000000
VERSION     DAOQEW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7201_length_4855_cov_8.194375"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..769)
                     /locus_tag="PLS35_17830"
     CDS             complement(98..769)
                     /locus_tag="PLS35_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNI20584.1"
                     /translation="MTTDNPAPLILCFGDSLTAGYQTPSPAHPSGQDSPYGYVLQEYL
                     GVRGRVEVSGICGETTGEMVLRFRRAVLERHPQMVIILGGTNDLGWNADPAEIMRNLI
                     KMYELARASSIVPVPVTVPSIRVDLGGENPDAAAWLAEHIQRRQRLNALIADYAQRKN
                     LRHVDLFTATAEPESLMLAEPYSNDGLHLTSSGYRVFGRLVYEELFAADPAVSGDASS
                     NLSQP"
     gene            877..>2213
                     /locus_tag="PLS35_17835"
     CDS             877..>2213
                     /locus_tag="PLS35_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20585.1"
                     /translation="MEPTAPVGEGLPLAPFVKPGTDPQVTAIKQLLKLLDKAAKSART
                     YGTKNPVAQRFFQQFYDELTNHLTQHTRLTLLVQRNQLLFQNEVVYEPERDATGDSFA
                     FKMYSDGIRELSFHQGLTHEDLAFFLEALWGMPADEASPADAAADQEEDDDIVTRLWA
                     KNLETITLVTAEELVRASGFGLDELELQTQGYMSLPVGSLRELLDRERAAKASDKEGT
                     GGSAEAAGGNTTGGIANRNRRFQANVVGYDVSDEEHEALTQEIQRETARDATTYILDI
                     LSAVLASEPSPALLTKLFDVWDHVLEALIRAGQWTLLETVLTMLQEAETVRPDLSDGH
                     KQQVAELFGGLGRPERLKAIGVHLNRNPQAKTEGLTTLLLMMKPDAIPGLCSVLATLE
                     TPAHQALVMEALQTLARDHAEPIIRGLTDKRPTFVRNLLTLIYRWNDARFADSV"
     assembly_gap    2214..2313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2314..2568
                     /locus_tag="PLS35_17840"
     CDS             <2314..2568
                     /locus_tag="PLS35_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20586.1"
                     /translation="TAADEAVPYWQGLLTEWSWTNRKKKEELALLAAEILGKLATPAA
                     MAALELGQKKGGAAVRQACSAAISVANRQHRHTIPSAANS"
     gene            2583..4184
                     /locus_tag="PLS35_17845"
     CDS             2583..4184
                     /locus_tag="PLS35_17845"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNI20587.1"
                     /translation="MTDSPSPVNPDPNSTDAAHPILTHERSLANKIAKGSEAGDILDQ
                     QLAMLGIQLVTQLNVLIKTSRIHGRTNAALDKPVDSMLTLVKTLAADHPVALRLQNDF
                     LFLGDSHLKMSSQQMAVFASIIEALNKWHIGGVSFASTAESKDYREFAYLFVSLDPEK
                     HTLADLQEALQTAGVNGIELEEPRALQLQQSNDADAMAGEGGTRVGSSAGGQENSKEK
                     RKAIAKGRYAKAASALGDLTKNTRAGGTVSFKQAKRAIQNIVELMMQDESTLLGLTNL
                     RCHDQYTHNHSVNVSLLAMALGNRAGYPKVDLADLGLSALFHDVGKCAISLDVLNKPG
                     EFTQDEWAIMRSHPIEGVFTLVKARGINNVPARMASASFEHHMNYDFSGYPKLKVPWS
                     QTVASRIITIADCYDAMTSSRVYRREPMSPANVLKFMFGKSGQSFDPVLLKLFVNCVG
                     IIPIGSLVRLDSGPLAVVIKPAQDKANAERPMVRVITDTEGTPIEDGPELDLAAQDDT
                     GTYTHNILQLVDNAEYHFDTGRYFV"
     gene            complement(4215..4616)
                     /locus_tag="PLS35_17850"
     CDS             complement(4215..4616)
                     /locus_tag="PLS35_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20588.1"
                     /translation="MSSEQEQAHRLFIRALQHEAISCERAGCPGPVETLDQSQTRDRV
                     KTFDMTCERCGWHTSVTGREQLTPLWDNASLELMADEHLLHQQPVCPYDDTPVVFISM
                     PNPRRKAKYRVACFYCGRHIEMDWPPPESKR"
BASE COUNT         1056 a         1463 c         1340 g          896 t
ORIGIN      
        1 agacagtgcc cgcagcccgg ctgccagaac tccacgagca ccggcatagg ggcctgctcc
       61 accgtacgcg cgaagttgtg atccgtgacc tcggagatca tggttgagac aggttggaag
      121 aggcgtcgcc ggaaacggca ggatcggcgg caaagagctc ctcatacacc agccggccga
      181 acacgcgata gccgctactc gtcagatgca gcccatcatt cgaatacggc tccgccagca
      241 tcagggactc cggttcggct gtcgccgtga acaaatcaac atgccgcaga ttttttctct
      301 gcgcatagtc cgcgatcagg gcattcaaac gctgacgccg ctgaatatgc tcagccagcc
      361 acgccgccgc atcgggattt tccccgccca ggtcgactcg aatagatggg acggtgacag
      421 gcacgggcac gatcgacgag gcccttgcca attcatacat cttgatcaga ttccgcatga
      481 tctccgcagg atcggcattc catccgagat cattcgtgcc ccccagaata atcaccatct
      541 gcgggtggcg ttcgagcacg gcccggcgga aacgcagcac catttcaccg gttgtttcac
      601 cgcagattcc gctcacctcg acgcggcctc gcacaccgag atattcctgt aagacgtaac
      661 catagggcga gtcctgtccg gacggatgtg ccggactggg cgtttgatat ccggccgtga
      721 ggctatcacc gaagcagaga atgagcggag ctggattatc ggtagtcacg gaagaattgt
      781 actggcacga acaggcgggc gcaatggcga acggagccct gcgcagaccg gctattttct
      841 tgacgaatca tgcccctcac ggtacgactg acacatatgg aacctacagc cccagttgga
      901 gagggccttc ctcttgcgcc cttcgtgaaa cccggcaccg atccgcaagt cactgccatc
      961 aagcagttgc tgaagctgct cgacaaggcg gccaaatcgg cgcgtacgta tggcacgaag
     1021 aacccggtcg cccagcgatt ctttcaacaa ttttatgatg agctgaccaa tcatctgacg
     1081 cagcacactc gcctcaccct gctggtccaa cgcaatcaac tcttgtttca aaacgaagtg
     1141 gtctacgaac cggagcggga cgccaccgga gatagctttg cgtttaaaat gtactcggac
     1201 gggatccgtg agctgagctt ccatcaaggg ctcacgcacg aagacctcgc cttttttctg
     1261 gaggcgctct ggggcatgcc cgcggacgag gccagtccgg ccgacgctgc cgccgaccag
     1321 gaagaagacg acgacattgt cacgcgtttg tgggccaaga atctcgaaac gattactctg
     1381 gtgaccgccg aagagcttgt ccgcgcctcc ggcttcggac tcgacgagct cgaactgcag
     1441 acgcagggat acatgagtct gcctgtcggc tccttgcggg aactccttga tcgcgagcgc
     1501 gcggcaaagg cctctgacaa agagggcacg ggcggctccg cggaggcggc gggcggaaat
     1561 accaccggcg gcatcgcaaa ccgcaaccgt cgtttccagg ccaatgtcgt cgggtatgac
     1621 gtctccgacg aagagcacga ggcattgacc caagaaatcc aacgggaaac cgctcgagat
     1681 gccacgacgt acatcctcga catcctttcc gctgtcctgg cctctgaacc atcccctgct
     1741 ctattaacca aactattcga cgtctgggat cacgtattgg aagcgctgat tcgggcagga
     1801 caatggacgt tgctcgaaac cgtgctgacg atgttgcagg aagcagagac ggttcggccc
     1861 gatctttcgg acggccataa acagcaggtc gcggaattat tcggcggact gggccgcccc
     1921 gagcgattga aagccattgg cgtccatttg aatcggaatc cgcaagccaa gacagaaggc
     1981 ctgacgacac tcctgctcat gatgaaaccc gatgcgattc cgggactctg ctcggtactg
     2041 gcgactctgg agacgccggc ccatcaggcg ttggtgatgg aagccctgca gaccttggcg
     2101 cgggaccatg ccgaacccat cattcgcggc ctgaccgata aacgaccgac tttcgtgcgc
     2161 aacctgctta cccttatcta ccgatggaat gatgcccgtt ttgccgacag cgtnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccgccg cggatgaagc cgtgccttac
     2341 tggcaaggac tgctgacgga atggtcctgg acgaatcgaa aaaagaaaga agagctcgca
     2401 ctattggcag cggagatcct cggcaaattg gccactcctg ccgctatggc ggcacttgaa
     2461 ctcggacaga aaaaaggcgg cgccgccgtc cgacaggcct gcagtgcggc tatctccgtc
     2521 gccaatcgcc aacaccggca tacgatcccc agcgccgcga attcctaaag acgaggaacc
     2581 ctgtgaccga ctccccttct cctgtcaatc cggacccgaa cagcacggac gccgcgcatc
     2641 cgattctgac ccatgaacgg tcgctcgcca acaagatcgc caaggggtcc gaggccggcg
     2701 acatcctcga tcaacagctg gccatgctgg gcattcagct ggtgacgcag ttgaacgtcc
     2761 tgatcaaaac gtcgcgcatc cacggacgca ccaatgccgc gctggacaaa cccgtcgact
     2821 ccatgttgac gctggtgaaa acgctggcag ccgatcaccc cgtggcgttg cgcttgcaga
     2881 acgacttttt gtttctcgga gacagccacc tcaaaatgag ctcgcagcag atggccgtct
     2941 ttgcgagcat catcgaggca ttgaacaagt ggcatatcgg cggagtgtcg ttcgcctcaa
     3001 cggccgagtc gaaagactat cgcgagttcg cctatctctt tgtcagtctc gatccggaga
     3061 aacatacgct ggccgatttg caggaagccc tgcagacggc gggcgtcaac ggtatcgaac
     3121 tggaagaacc acgagcactt caactccagc aatcgaacga cgccgacgca atggccggtg
     3181 aaggagggac gcgggtagga tcctcagccg gcggccagga aaattcgaag gagaagagga
     3241 aggccatcgc caaaggtcgt tatgcgaagg cggcctcggc gctcggcgac ctgacgaaga
     3301 atacgcgcgc tggagggacg gttagcttca aacaggcgaa acgcgcgatt cagaacatcg
     3361 tcgaactgat gatgcaggat gaatccacac tcctgggact gaccaatctt cgctgccacg
     3421 atcagtatac ccataaccat tccgtgaacg tgtcactcct ggcgatggcc ctgggcaatc
     3481 gagccgggta cccgaaggta gacctggccg atctcggttt gtcggcgttg tttcacgacg
     3541 tagggaaatg tgccatttct ctggatgtct tgaacaaacc cggcgagttc acgcaggacg
     3601 aatgggccat catgcgctcg cacccgattg aaggcgtgtt taccctcgtg aaggctcggg
     3661 gcatcaacaa cgtgcccgcg cgcatggcct cggcctcctt cgaacatcac atgaactatg
     3721 atttttctgg ctaccccaaa ctcaaggtgc cctggagtca aaccgtcgcc agtcgcatca
     3781 ttacgatcgc cgactgctac gatgcgatga cgtcgtcgcg cgtataccgc cgggagccga
     3841 tgtccccagc aaacgtgctc aaattcatgt tcgggaaaag cgggcaatcg ttcgaccccg
     3901 tgctgctcaa actgttcgtg aactgcgtgg gcatcattcc catcgggagc ctcgtgcgcc
     3961 tcgactccgg tcccctggcg gttgtgatca agcccgctca ggacaaagcc aacgcggaac
     4021 gtccgatggt gcgagtgatt acggacacag agggaacgcc gatcgaggat ggtcccgagc
     4081 ttgacctggc cgcgcaggat gacaccggaa cctacacgca caacattctc cagctcgtcg
     4141 acaacgccga gtaccacttc gacaccggtc gctacttcgt ctaacccacc ggcatctcca
     4201 ttcaagcctc gtggctaccg cttgctttca ggcggcggcc aatccatttc aatgtgtcgc
     4261 ccacaataaa agcacgccac ccggtacttc gccttccggc gcggattcgg catggagata
     4321 aacacgaccg gcgtgtcgtc atatggacat acgggctgtt gatgcagcag atgttcatct
     4381 gccatcagtt ccaatgaggc attgtcccag agcggcgtga gctgttcgcg cccggtcacg
     4441 ctggtgtgcc acccgcatcg ttcgcaggtc atatcaaacg tcttgacccg atcgcgtgtt
     4501 tgcgactggt cgagggtctc taccggcccc ggacatccgg cgcgctcgca gctaatcgcc
     4561 tcatgttgaa gcgcccggat aaataagcga tgggcctgct cttgctcgga agacatgata
     4621 gatttccttt cccatccggc cctgggaccc ggccggatgt gcagacgatg agtgcctgcg
     4681 ttggcttata gccaccggtc ccaggcctgt caaatccccc actcaccccg ccgctccctc
     4741 cttgacacga tacgggcagg cagctatcct cccgaggttt gtagcacgac attattcccg
     4801 cgaaaggagt cccgagatgg gtgtgaagtg gcacggagca ggccggcgcg cggtc
//
LOCUS       DAOQEW010000254         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7350_length_4800_cov_5.473340, whole genome shotgun sequence.
ACCESSION   DAOQEW010000254 DAOQEW010000000
VERSION     DAOQEW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_4800_cov_5.473340"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..324)
                     /locus_tag="PLS35_17855"
     CDS             complement(79..324)
                     /locus_tag="PLS35_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI20589.1"
                     /translation="MDQPLLRVKEAAQLLQVSKWTIYRWIDEGRLEATKIGGGSLRIF
                     RRSVAALVEGNRTDARSTDFPPQLKVVPLSGRRAAKR"
     gene            complement(690..1022)
                     /locus_tag="PLS35_17860"
     CDS             complement(690..1022)
                     /locus_tag="PLS35_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20590.1"
                     /translation="MIRRISQTTVAGLALGIVWVYMVLALVAPGCSFAHAAPSDGHHQ
                     HSGTESHSTLCSWACQATSDVGPISQGDVGMAWVAQIQPLISPPILLTSWDPSSLHAR
                     APPVSARG"
     gene            complement(1131..2300)
                     /locus_tag="PLS35_17865"
     CDS             complement(1131..2300)
                     /locus_tag="PLS35_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20591.1"
                     /translation="MLWSAYSLRNLWARRVTTLLTLLGLGLVVFVFVAILMLAHGLER
                     TMGRTGDPANAIVMSKGALSEIESSLSREQTGVLAAQPEVITLADHQAAAVREIALQL
                     TLRKQEGGSLASLSLRGSSPDAFAVRPLVRIVQGRLWQPGTTEVIVGTQVAKQFPDAR
                     LSQTLRFGRREWTVVGIFEAEGSGFDSEVWGDAEQMMATFHRTAFSSMTVRLADPTVL
                     PAFKARLERDPRFKVSVKREPEYYEGKAEGLARLIRVTGLFLTVVFSVGAILGATMTM
                     SASVAHRTTEIGTLRTLGFTRSHILQAFLLESILLGLAGGLLGGAGAALLNSTTISTV
                     NWDTGAELAFAFHLTPTMVGEGLLFAAGMGVIGGLVPAARAARIEIVHALGQRTV"
     gene            complement(2318..3484)
                     /locus_tag="PLS35_17870"
     CDS             complement(2318..3484)
                     /locus_tag="PLS35_17870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20592.1"
                     /translation="MTLIRLMFRNTVRRPVRLALTVGGLAMVVLAFGLLRLTLDEWHS
                     GVKAAANNRLITVHAMSDSLLLPLSYRDRMSVVPGVQVVHFGNVFGVEYRDAKESFGA
                     FAEQMSTFFETYPEVILNEGDRLAFLKDRGGCLIGQKLAARFGWKVGDVIPLKGTTHP
                     GNWDLVVRGIFRSSSPGIMGEGELYVHWEFANERLRSTAPDRADQVGWYVITTAPGVN
                     PRTVVAAIDATFANSFAETRSEREQAYIAGWVARSGALLDALDWLSGVMNGIAALVLV
                     NALAMAVRERTREYGVMKTLGFQPRHLVALVLGESLLVALGGALAGLGLLYPAARLYA
                     VMVAGGKTVGAYEITFETIGMCLGVMGLVGLMAALWPAVRLSRMTTLEGLRHRG"
     gene            complement(3481..4164)
                     /locus_tag="PLS35_17875"
     CDS             complement(3481..4164)
                     /locus_tag="PLS35_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI20593.1"
                     /translation="MVDPVVSLQKITKSYSRAGIEVSVLRDLSFDIPAGTFLAIMGPS
                     GSGKSTLLNVMAGIDRPTGGSVVVAGTRLDGLSEGEMARWRARHIGYVFQTYNLIPVL
                     TAAENVELPLALTHLTRRERADHVKTALRLVGLGDRMDHYPRQLSGGQEQRVGVARAI
                     VSDPTMILADEPTGNLDRESADDILTLLARLNRELGKTIVMVTHDPRAAERAQMIRHL
                     EKGVLEMPP"
     gene            complement(4176..>4800)
                     /locus_tag="PLS35_17880"
     CDS             complement(4176..>4800)
                     /locus_tag="PLS35_17880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNI20594.1"
                     /translation="QLENTSIRAPFDGVIVKKLAEIGEVVSPFTATVRSGGSVVNMVD
                     PLSVTVDAEVSEAMIHRVRAGQPAEIQLDSVPGHRYQGEVQQVMPIADRAKGTVLTRI
                     RFRDLDDRVRPELSAKVTFGPVPGAQTEPGEDWGVPSTAVVTRDGRAVVLVVRAGIAA
                     EAVVQAGTPAAGMVPVHGPLSQTDEVIVAPTEHIRSGTTVSVFRRAS"
BASE COUNT          992 a         1651 c         1361 g          796 t
ORIGIN      
        1 ggggaggccg tcccattcac cccggggagg cagatcctga agaagggtgc acaactccag
       61 gatccgtcta tgcttcgctt atcgcttcgc ggcccgtcgt ccgctcaacg gcaccacctt
      121 caactgcggt gggaaatccg tcgatcgcgc atcggttcga ttgccttcga ccaaagccgc
      181 cacggaccga cgaaaaatgc ggagactgcc tccgccgatc ttggtcgcct ccagacgccc
      241 ctcgtcgatc cagcgataga tcgtccattt gctgacttga agcaattgcg cggcttcctt
      301 gacgcgcaag agcggctgat ccatcacttt cactcctcca acgtttggcg gtggacagga
      361 gcggttgcga caccgctcct gtcccgaatg cacgaggggg acgagcctct cgaacaatcg
      421 ggtccaccat gcacgacacc atcacacacg tgaccagtga tgagtgggaa ccggcagcgg
      481 aggtcacagg gaaggcgcct gcgctgccgg ttattccggg gtagagggca attcgacccc
      541 ggactcacag gcactgagtg aaatagctcg ggccgaacgc ccggagacgg cggattacag
      601 gacaacctca ctcccgccag cggcgcgcac cgcaacgaaa gcacaggttt cgaactctgc
      661 aatgaaaggc gacgtgcgcc ccgcccgaac tatcccctcg cggagaccgg cggcgcacgg
      721 gcgtggaggg acgaagggtc ccaggaggtc aggaggatcg gaggagaaat cagcggttga
      781 atctgtgcca cccaggccat ccccacatcg ccctgggaaa taggaccgac atctgatgtc
      841 gcctgacagg cccaggagca gagggtggaa tgggattccg tgcctgaatg ctgatgatga
      901 ccatccgacg gcgcggcatg ggcaaaggaa cagccaggcg ccacaagggc caacaccata
      961 tacacccaga caattccgag cgcgaggccg gcgactgtgg tttgtgagat tcgtcggatc
     1021 atggtctgag agtgctcacc tggcgtatca attacgccgt tccacagaag ttgtctaccg
     1081 acggatgctc acgcctgcta caaattccgg ggggaaggcc cggtcaccac ctacaccgtc
     1141 cgttgtccga gcgcgtggac aatctcgatg cgggccgccc gtgcggcggg cacgagcccc
     1201 ccgatgacgc ccatgccggc agcgaataac aatccttcac cgaccatagt aggagtgaga
     1261 tgaaatgcga aggccaactc ggcgccggta tcccagttca ccgtggaaat cgtcgtggaa
     1321 ttgagcaggg ccgccccggc cccacccagc aaacccccag cgaggcccaa tagaatcgat
     1381 tcgaggagaa aggcttgcag aatgtggctg cgcgtaaatc ctaacgtgcg gagcgtgccg
     1441 atctcggtcg tgcgatgcgc gacagaggcg gacatggtca tcgtcgctcc gagaatggcc
     1501 ccgacgctga acaccaccgt cagaaataaa ccggtcacac gaatcaacct ggccagccct
     1561 tcggccttcc cttcgtaata ttccggctcg cgcttcactg acactttgaa ccggggatca
     1621 cgctccagcc gcgctttgaa tgcggggagc acggtcggat cagccagccg tacggtcatc
     1681 gacgagaagg ccgtccggtg aaacgtggcc atcatctgct ccgcatcgcc ccacacctcg
     1741 gaatcgaacc cgctgccctc cgcctcgaaa atcccgacca ccgtccactc ccgccgcccg
     1801 aaccgcagcg tttgggataa acgggcatca ggaaactgct tggccacctg ggtgccgaca
     1861 atcacctccg tcgtgcccgg ctgccacaga cggccttgga cgatgcgcac cagaggacgc
     1921 acggcaaagg catcgggcga cgagccccgc aatgagagac tggccaggct cccgccttcc
     1981 tgtttgcgca aggtgagttg cagcgcaatc tcccgcaccg ccgcggcttg atgatctgcc
     2041 agagtgatga cctccggctg tgccgccagc actccggtct gctcgcgcga caagctgctt
     2101 tcgatttcgg agagcgcccc cttgctcatc acgatcgcgt tggcaggatc cccggtcctg
     2161 cccatcgttc gctccaagcc gtgcgccagc atcagaatcg ccacgaacac gaagaccacg
     2221 agcccaagcc ccaacagcgt gagcagcgtc gtgacgcggc gcgcccagag attccgcagg
     2281 ctgtatgcgc tccacaacat gggccgacct cgcgacgtta tcccctgtga cgcaaccctt
     2341 ctaaagtcgt catgcggctg aggcgcaccg cgggccagag agcagccatc agcccgacca
     2401 gccccatcac accgagacac attccaatcg tctcgaaggt gatctcgtac gcgcccactg
     2461 ttttcccacc ggcgaccata accgcataga gccgggcggc ggggtacaac aatcccaacc
     2521 cggccagggc gccgcccagc gccaccagca gcgattcccc caggaccagc gccaccaaat
     2581 gacgaggctg gaagcccagg gtcttcatca ctccgtactc cctggttcgt tcgcgcaccg
     2641 ccatcgccag cgcgttgacc aagaccaggg cggcgatacc gttcatcacg ccggacaacc
     2701 aatccaacgc atcaagcaac gcgcccgagc gcgccaccca gccggcgata taggcctgtt
     2761 cccgctccga cctggtttcg gcaaatgagt tggcaaaggt ggcatcgatc gcggccacca
     2821 cagtacgggg attcacgccc ggagccgtcg tgatgacata ccagcccact tggtcggcac
     2881 ggtccggtgc cgtggacctc aaccgctcat tggcaaactc ccaatggaca tagagttccc
     2941 cttcgcccat gatgccaggg ctgctgctgc ggaagatgcc gcgtacgacc aggtcccaat
     3001 tcccgggatg ggtcgtaccc ttgagcggga tcacgtcacc gaccttccaa ccgaatcggg
     3061 cggcgagttt ctgtccgatg aggcatccgc cccgatcctt cagaaacgcc aggcggtctc
     3121 cttcgttcaa gatgacttcc gggtaggtct caaagaacgt gctcatttgc tccgcaaacg
     3181 ccccgaacga ttccttcgca tctcggtact cgacgccaaa tacatttccg aaatgcacta
     3241 cctgcacacc ggggaccacc gacatccgat cccgatacga caacggcagc agcagggaat
     3301 cggacatggc atggacggtg atcaagcgat tgttggccgc cgccttgacg ccgctgtgcc
     3361 attcatccag cgtcaaccgc aacaacccga aggccaacac gaccatcgcc aggccgccga
     3421 cggtcagcgc cagacgtacc ggccgtcgga ccgtgttccg aaacatcaag cggatcagcg
     3481 tcatggcggc atctccaaca ctcccttttc gagatgcctg atcatctgtg cgcgttcggc
     3541 cgcgcggggg tcgtgggtga ccatcacgat ggtcttgccc aattccctgt tcaaccgagc
     3601 caacaacgtc agaatgtcat cggcagattc gcggtcgaga tttccggtcg gctcgtccgc
     3661 cagaatcatg gtggggtcgc tgacgatggc gcgcgccacc ccgacccgct gctcctggcc
     3721 accagacagc tgccggggat agtgatccat gcgatcaccc agcccgacca gccgcagcgc
     3781 ggtcttcacg tggtccgcac gctcccggcg tgtgagatgc gtcagcgcca acggcagttc
     3841 cacattctcc gccgctgtca gcaccgggat gaggttgtag gtctgaaaca catagccgat
     3901 gtggcgcgcg cgccaccgcg ccatttcacc ctccgacaag ccgtccagcc tcgttccggc
     3961 caccaccacc gatccgcccg tcggccgatc gatcccggcc atcacgttca gcaacgtgct
     4021 cttgccggac ccggacggcc ccatgatcgc gagaaaggtg ccggccggaa tatcgaagga
     4081 cagatcccgc aggaccgaca cctcgatgcc ggcacggctg tagctcttcg tgatcttctg
     4141 gagactgacg accggatcaa ccatgggtga actcgttacg aggcacgacg gaagactgac
     4201 accgtggttc cggaacgaat atgttcggtc ggcgcaacta tgacctcatc ggtctgcgac
     4261 aaaggtccat gaacaggaac catcccggct gcgggagtcc cggcttgcac caccgcttcc
     4321 gccgcgattc ccgcgcgcac gaccaagacc accgctcgcc cgtcgcgcgt caccacggcg
     4381 gtggacggca caccccaatc ttcccccggc tctgtctgtg ctcctggaac aggaccgaac
     4441 gtcaccttgg cgctcagctc aggccgcacc cgatcatcca ggtcacgaaa tcggatgcgc
     4501 gtcagcaccg tccctttggc gcgatcggcg atcggcatca cctgctgcac ttcgccctga
     4561 tatcggtgcc ccggcaccga atcgagttga atctccgccg gctgcccggc ccgcacgcgg
     4621 tgaatcatcg cttccgacac ctccgcatcg accgtcacgg acaaggggtc gaccatgttc
     4681 accacggacc cgccggaacg gacggtggcg gtgaacggcg acacgacctc gccgatctcc
     4741 gccaatttct tcacgatgac tccgtcgaac ggcgcgcgaa tgctcgtatt ctccagctgc
//
LOCUS       DAOQEW010000255         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7403_length_4780_cov_9.064127, whole genome shotgun sequence.
ACCESSION   DAOQEW010000255 DAOQEW010000000
VERSION     DAOQEW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7403_length_4780_cov_9.064127"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLS35_17885"
     CDS             <1..234
                     /locus_tag="PLS35_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20595.1"
                     /translation="EQRADLFRQSPVLSGRLRVSEQTLIPYIGAGFGGGYVTERDRAL
                     NPQPTLPQQNLFGEAMGKSMMPNEFQMGIRIPF"
     gene            complement(283..966)
                     /locus_tag="PLS35_17890"
     CDS             complement(283..966)
                     /locus_tag="PLS35_17890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNI20596.1"
                     /translation="MRAIIFDFNGVIADDEMTHLECFQKALREAGLVLSKEDYYGTYL
                     GMDERTCAAAMLTRRDGTCDHTTLARIIERKAALFRTHTATHKPALFPWVTGFVQRAV
                     EQYRLAIASGGRREQIQSALAGHPIEAAFEVMISADDCAVGKPDPAIYECALRQLAAK
                     RPKPPLLRADECLVIEDSRAGIQAGLRAGMRVLALATTYPASQLTEAHLVLPSLEGID
                     PSELARRLF"
     gene            1134..3158
                     /locus_tag="PLS35_17895"
     CDS             1134..3158
                     /locus_tag="PLS35_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNI20597.1"
                     /translation="MSDYAVLGDLLVIYAVSTAVVFVFHQFRLPSIAGFLVAGALIGP
                     HGLHLIPDVSQVQVLAEIGIVLLLFTIGMEFSSAHFASARRILAVAAPVQTGGVLILA
                     LLGALAVGLSYQQGIFWGFLLSLSSTAIVLKALSEQGESDSFHGRATVAILIFQDLAV
                     VPMMLITPILATPSGSAMGMVLLTLLKAAVVVVLIVAAAWYLVPRLLRHIVRSRSREL
                     FLLSIIVLCLGIAWLTSLGGLSLALGAFIAGLVMSESEYSHQALAEVLPFRDSFNSLF
                     FVSIGVLMDLRVVLDHPFIVLGLLVAVIAGKLLAGAGAMVASGAPPRSAVLVGVALAQ
                     VGEFSFILAQQGQEAGLFSGDHYQLFLAVSVLTMVVTPFVMQWSPHLGRRIEALQRVR
                     GWMPTRTVAHVEQLEGGQVRIKDHVIIVGYGLNGRNLARVLSETEIPHVALDLDGETV
                     RRESRHGVPIYYGDGSNANVLRHMKIEDAKVLVIALSDPFTARRTVKVAKGLNAKLHI
                     VVRTRYLRELEELHQLGADDVVPEEFETSIEIFALVLRTYCLPQEFVARKAEQIRREG
                     YALLRRSEMPELAHHLRGGTLTDVEVETCRVDEDAPAVGKSLTELSIRPRTGASVIAW
                     TRSQVTQSNPSETVRLQAGDVVTLLGSREQIRRAMALLNEPNHGTSHQLT"
     gene            complement(3217..>3504)
                     /locus_tag="PLS35_17900"
     CDS             complement(3217..>3504)
                     /locus_tag="PLS35_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNI20598.1"
                     /translation="QCRIDFTAAWNILPWLPFAKPLFRYFANIFMKQDKQAMERQAVG
                     LKYKPALMLIDDADTPAKWYYKLKAAHLASVQTGRPLEHPLKGPVTLRWRS"
     assembly_gap    3505..3604
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3605..4026)
                     /locus_tag="PLS35_17905"
     CDS             complement(<3605..4026)
                     /locus_tag="PLS35_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HNI20599.1"
                     /translation="MNTDLITELKPTKSAPLFGFWYPAATSSSLGVGQMQTQVMLGLP
                     ILICRDRQGQVAAMRDICPHRGMPLSFGHFDGERVECAYHGWQFDTGGRCRHIPALVE
                     GSPLQAEKIGITSYPCRDEDGYVWVFLPDPQQPNQPVP"
     gene            4252..>4780
                     /locus_tag="PLS35_17910"
     CDS             4252..>4780
                     /locus_tag="PLS35_17910"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNI20600.1"
                     /translation="MTTAQIEPTAALAELRESKADRVTIVLLSGDLDKAMAAFIIATG
                     AAAMGMRVTVFFTFWGLNTIRKKGASSSASDWLRRMFGFLNKGGADTLPLSRFHFWGL
                     GTRMMQVVMKQNRMPGVPELMQMAIDLDVRFIACTTTMGLMGITKDTLIDGIDQFAGV
                     TTYLAEAKQGSVNLFI"
BASE COUNT          874 a         1277 c         1473 g         1056 t
ORIGIN      
        1 gaacagcgcg cggacctgtt ccggcaatcg cctgtgttga gcgggcgatt acgtgtcagc
       61 gaacagacgt tgattccata catcggcgcc gggttcggtg gggggtacgt gacggagcgt
      121 gatcgggcct tgaatccgca gccgaccttg ccgcagcaaa atctcttcgg cgaggctatg
      181 ggcaaaagca tgatgccgaa cgagtttcag atgggcattc gcattccctt ctaatcctgc
      241 cggttctgtt cgccctgtcg agtaagactt tctcatcact ggttagaaca gccgccgggc
      301 caactctgac ggatcgattc cttccaggct cggtaggaca agatgcgctt cggtcagctg
      361 tgatgccggg taggttgtcg ccagggcgag cacgcgcatc cctgcccgta agcccgcctg
      421 aatgcctgca cgggaatcct cgatcaccag gcactcatcg gctcgcagga ggggcggttt
      481 gggtcgcttg gcagcgagtt gccgaagcgc acattcgtaa atcgcgggat cgggcttacc
      541 gacggcgcag tcatcggcgg agatcattac ctcaaacgca gcctcgatgg gatgaccggc
      601 gagagccgac tggatctgtt ctctcctgcc gcccgacgcg attgccagcc ggtactgctc
      661 aactgcccgc tgcacgaagc cggtcaccca gggaaacaat gcgggtttgt gcgtcgctgt
      721 gtgggttcga aaaagtgccg cttttcgttc gatgattcgg gccagggtgg tgtggtcaca
      781 agtgccgtcc cgtctggtga gcatcgcggc tgcgcaggtc cgttcatcca tgccgaggta
      841 ggttccgtaa tagtcctcct tgctcaggac gagtccagcc tcgcgcaacg ccttctgaaa
      901 acattccagg tgcgtcatct catcgtcggc gatgacgcca ttgaagtcga aaatgatcgc
      961 gcgcaagatc cctccgtaac gtctctccaa tgtgccgcta agcgcgtcac gttagctgag
     1021 tttcctggtg cgggacaagc agaactcctt gttccattga gatccctacg gtatgctgcg
     1081 tcacgaatgc gtccaacctc cgatgtaact gattgtccaa atcttgtgac tctatgagtg
     1141 attacgcggt tctcggcgat ttattggtga tctatgcggt ctcgaccgca gtggtgttcg
     1201 tctttcacca gttccggttg ccgtcgatcg ctgggtttct ggtggcgggc gcgctcattg
     1261 gtccacatgg gttacatctc attcccgatg tgtctcaagt gcaggtgttg gccgaaatcg
     1321 gcatcgtctt attgctcttc acgatcggca tggagttttc ctcggcacac tttgcctcag
     1381 cccgccgcat tctcgcggtg gccgcgccgg tgcaaaccgg aggggtgctc atcctggccc
     1441 tgctcggggc gttggccgtg gggctctcct atcagcaggg catcttctgg ggctttctcc
     1501 tgtcgctcag tagtacggcc atcgtgctga aagccctgtc cgagcagggc gagagtgatt
     1561 cgtttcacgg ccgtgccacc gtggccattt tgattttcca ggacctggct gtcgtgccga
     1621 tgatgctcat caccccgatc ctggcgacgc ccagcgggag tgccatggga atggtgttgc
     1681 tcactctgct caaggcggcg gtcgtagtgg tgttgatcgt cgctgcggcc tggtatctcg
     1741 tgccgcgatt attgcggcat atcgtgcgaa gtcgcagccg tgagctcttc ctgctctcca
     1801 ttatcgtgct ctgtctcggt attgcctggc tgacgtcgtt gggaggattg tccctggcct
     1861 tgggcgcatt tatcgccggg ttggtgatgt ccgaatcaga gtacagccat caggcgttgg
     1921 ccgaggtgct gccgtttcgc gacagtttca atagtttgtt tttcgtgtct atcggcgtgt
     1981 tgatggatct acgggtggtg ttggaccatc cgttcattgt gctggggctt ctggtggctg
     2041 tcattgcggg gaagctgctg gccggcgccg gagcgatggt ggcatccggc gcgccgccca
     2101 ggtcggccgt gctggtcggc gttgcgctcg cgcaggtcgg agaattcagt ttcattctcg
     2161 cgcagcaggg ccaggaggcc ggcttgttct ccggggatca ctatcagtta tttcttgctg
     2221 tgtccgtgct cacaatggtg gtgacgccgt ttgtgatgca atggtcgcct cacctgggcc
     2281 ggcggatcga agcgctgcaa cgagttcgtg gctggatgcc cacccgcacg gtggcgcacg
     2341 tcgagcaatt ggaggggggg caggtccgga tcaaggatca tgtgatcatc gtgggatacg
     2401 gcctcaatgg tcgcaatctt gcccgcgtgt tgagcgaaac cgaaatccct catgtggcgc
     2461 tcgatctgga tggcgaaacg gtgcgccggg agtcgcgcca tggcgtcccg atttattacg
     2521 gcgatggctc gaatgcgaat gtcctccggc acatgaaaat cgaggatgcg aaggtcctcg
     2581 tgattgctct atccgatcca tttacggctc gaagaacggt gaaagtcgcg aagggcctca
     2641 atgcgaagct acacatcgtg gtgcggacgc gatacctgcg ggagctggaa gaactgcatc
     2701 agctgggggc ggatgacgtg gtgccggagg agttcgagac gtcgattgaa atttttgccc
     2761 tggtattgcg cacctattgt ctcccgcagg aattcgtggc cagaaaagct gaacaaatca
     2821 ggcgggaggg gtatgccctg ctgcgtcgca gtgaaatgcc ggaattggcg catcatttgc
     2881 gcggggggac gctgaccgat gtcgaggtgg aaacctgccg ggttgacgaa gacgctcccg
     2941 ccgtgggcaa atcgttaaca gagttgtcga ttcgtccgcg aaccggcgcc tcggtgatcg
     3001 cctggactcg cagtcaggtc acgcaatcga acccgtctga aaccgtgcgg ctccaagccg
     3061 gcgacgtcgt gacgttgctg ggatcgcggg agcaaatccg ccgggccatg gcgctcttga
     3121 acgagcccaa ccacggaacg agccaccaac tcacctagcg ccctcagcgc gcaggtcttg
     3181 tcaggtaccg cccacttcag gcagccgagg ctgttgctag cttctccacc gcagtgtgac
     3241 cggacctttg agcggatgct ccaacggacg tccggtttgc acggaagcga gatgggcggc
     3301 tttcagcttg tagtaccact tggccggggt gtcggcgtcg tcgatcagca tgagtgcagg
     3361 tttgtacttc aggccaacag cctgtcgctc catggcctgt ttatcctgct tcatgaagat
     3421 attcgcgaaa tatcggaaca ggggtttggc gaaggggagc cagggaagga tattccaggc
     3481 ggcggtgaaa tcgatccggc attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnntccggc accggttgat tgggctgttg cggatcgggg agaaagaccc acacgtatcc
     3661 atcttcatcc cggcagggat aggatgtgat gccgattttc tctgcttgaa gtggggagcc
     3721 ttcgaccaaa gcggggatat ggcggcagcg gccgcccgtg tcgaattgcc agccatgata
     3781 cgcacattcg acgcgctcgc cgtcaaaatg gccaaatgac agcggcattc cgcgatgggg
     3841 acaaatatcg cgcatggctg cgacctgccc ctggcgatca cggcagatga ggatgggcaa
     3901 tcccaacatg acctgcgtct gcatctgtcc gacgcccaag gaactgctcg tagcggctgg
     3961 ataccagaag ccgaataacg gcgcactttt cgtgggtttc agttcggtga tgaggtctgt
     4021 attcatggat acccgggata tgaccagggg cttcgcggac gagtggacta taacggcggg
     4081 tctgcaacag gtcaagcaat ggatcgcaag gggaaacgtc tgcagtgctg cgtcaggagc
     4141 gccggggagc gccgttacgg cttcgcgttt cttgacaaaa aaaagaagtg gagactatag
     4201 tcatttttga tgcgaattca gtcgtgtgtg ctcagacagg cgaggtaatc catgacgacc
     4261 gcacaaatag agcccacagc cgccctggcg gaactccggg agtcgaaggc tgaccgcgtc
     4321 accatcgtgc tcctcagcgg agatctcgat aaagcgatgg cggcgttcat catcgcgacg
     4381 ggcgccgccg cgatggggat gcgcgtgacg gtatttttta ccttctgggg gctcaacacg
     4441 attcgcaaaa agggggcgag cagttccgca tcggattggc ttcgtcgcat gttcggcttc
     4501 ctcaataaag gcggggccga cacattgccc ttgtcccgtt ttcatttctg ggggctgggc
     4561 acgaggatga tgcaggtcgt catgaagcag aatcgtatgc ccggcgtgcc ggagttgatg
     4621 caaatggcga tagatctcga tgtgcgcttt attgcctgca ccacaaccat gggcttgatg
     4681 ggaatcacga aagatacgtt gatcgacgga atcgaccaat ttgccggtgt caccacctac
     4741 ctggcggaag ccaagcaggg cagtgtcaat ctgtttatct
//
LOCUS       DAOQEW010000256         4660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7712_length_4660_cov_9.382193, whole genome shotgun sequence.
ACCESSION   DAOQEW010000256 DAOQEW010000000
VERSION     DAOQEW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_4660_cov_9.382193"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLS35_17915"
     CDS             complement(<1..316)
                     /locus_tag="PLS35_17915"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20601.1"
                     /translation="MDSIRNPFAPGAGTPPPELAGRDELRETLRVALERARIGRPAKS
                     AMLVGLRGVGKTVLLDRIRTDAEAAGIHTVRIEAPESRSLPALLAPQLRQALLRLSRL
                     QAA"
     gene            513..920
                     /locus_tag="PLS35_17920"
     CDS             513..920
                     /locus_tag="PLS35_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20602.1"
                     /translation="MTDAEPPHFDAATLRALAESWDSAAATACTACGPLICPGWESWP
                     ATADPQALQRVGSLRDASIDDPTLDEHHPAGTHAWDATAPIAPAFHPYNRCDVWQCRH
                     CARPFLRYTEYGGYYQDERVRELTAGLIVDALP"
     gene            1422..2348
                     /locus_tag="PLS35_17925"
     CDS             1422..2348
                     /locus_tag="PLS35_17925"
                     /inference="COORDINATES: protein motif:HMM:NF038232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family effector protein"
                     /protein_id="HNI20603.1"
                     /translation="MREVLFSKIDLSASRLKPYEGFIFLCGGPTGVAATHPVSIRDAI
                     YRELAKDKDIDRRIRVAEHYKDWSSEATYTDLVSFERHLAELSSVIVLVLESAGSIAE
                     LGLFSVIEEFKKKLLVFIETEHYKTDSFIRLGPIDYLEKNCNNSAECHRWMKGSGRAA
                     VFDQIAASDLQTDLAAAVRFRASLPSPERQLHPSGWLDGALLVCDLLNICSALTIREL
                     KQLLDGLGCQRNESEVKQLLFLLTRMGLLAMEPKGDQRFYVSTDAREFVRFGMRDATF
                     DLMRFRSDLLDLYQRADKKRFRAIQEARARHA"
     gene            2341..3252
                     /locus_tag="PLS35_17930"
     CDS             2341..3252
                     /locus_tag="PLS35_17930"
                     /inference="COORDINATES: protein motif:HMM:NF038233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family RNA-directed DNA polymerase"
                     /protein_id="HNI20604.1"
                     /translation="MHSDTDLIRQIARDMGMLPGHLASLIRTAPLRYKVFFIEKKSGG
                     LREVAQPAREVKVVQRWLVRHLTPHLPVHEAATAYRVGASIRDNAARHASSRYMLKLD
                     FTNFFPSIVRSDISAHLERHCGQLYDSSARLLIAHTCCWARKRTPPLRLCIGAPISPL
                     MSNTIMFDFDKRLASFAEADGVTYSRYADDLTFSTDRPHVLDRYVSLVRELLDEISYP
                     SLQLNDEKTVFASRSARRTVTGLVITPDGSVSVGRDRKRLIRSMYHRGTLGLLSPDET
                     KELIGLIAFVDSIEPGFSQRLRASGST"
     gene            complement(3545..3961)
                     /locus_tag="PLS35_17935"
     CDS             complement(3545..3961)
                     /locus_tag="PLS35_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNI20605.1"
                     /translation="MIILDTNVVSEPLKPEPSAAVLAWLDAQPVGSLFLTTVSWAELL
                     AGVAALPQGRRRTELHRALTQQVRPLFEDRVLDFDTAAAEAFASVHAAAKAAGNPIAF
                     ADGAIAAIAAARGFTIATRNVRDFYGTGVRMVNPWD"
     gene            complement(3958..4200)
                     /locus_tag="PLS35_17940"
     CDS             complement(3958..4200)
                     /locus_tag="PLS35_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HNI20606.1"
                     /translation="MPAITVRNLSDETHRALKLLATLHGRSTEAEVRRILDEAVRPRT
                     GLGTALARIGQELGGVDDLELNLQRDKTPIEPATFE"
     gene            complement(4274..>4660)
                     /locus_tag="PLS35_17945"
     CDS             complement(4274..>4660)
                     /locus_tag="PLS35_17945"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HNI20607.1"
                     /translation="PRIANRLLRRVRDYAEVKGDGRIIKAIADKALAMLDVDPQGFDL
                     MDRKLLESVVHRFDGGPVGLDNVAAAIGEEPGTIEDVIEPYLIQQGFLQRTPRGRIAT
                     LAAYRHLGVAPPNRAGLAGAGDLFEG"
BASE COUNT          867 a         1470 c         1386 g          937 t
ORIGIN      
        1 tggctgcctg cagccgactc aggcgcagca aggcttggcg cagttgtggg gccaacaggg
       61 cgggcagcga gcggctttcc ggcgcctcga tgcgcacggt gtggatgccc gcggcctcgg
      121 catcggtgcg aatgcggtcc agcagcaccg tcttgccgac gccacgcagc cctaccagca
      181 tggcgctctt ggccgggcgg ccgatgcggg cgcgctccag cgccacccgc agcgtctcgc
      241 gcagctcgtc ccgaccggcc agctctggcg gtggcgtgcc tgcaccgggc gcgaaggggt
      301 tgcggatcga gtccatgcgg gctcctttta ttcggatctt tggaagttta tgtgattgcg
      361 ataactttcc aaagatccgc ataactgcag atccatgccg ggccactgcc ccttgttcgt
      421 ccagccttac gcagccgaat cgccgcgttc cagcgggccg cagcgcggtc ccggccagaa
      481 tgacgccaag acagcccagg ccacccccgc ccatgaccga tgccgaaccg ccccacttcg
      541 acgccgcaac cctgcgcgca ctggccgagt cctgggactc cgcggcggca acggcctgca
      601 ccgcctgcgg cccgctgatc tgccccggct gggaatcctg gccggccacc gccgacccgc
      661 aggcgctgca gcgcgttggc agcctgcgcg atgcgtcgat cgacgacccg acgctcgacg
      721 agcaccaccc cgccggcacc cacgcctggg acgccaccgc gcccatcgcc ccggccttcc
      781 acccctacaa ccgctgcgac gtctggcagt gccgccactg cgcacggccc ttcctgcgct
      841 acaccgagta cggtggctat taccaggacg agcgggtgcg ggagctgacg gctgggctca
      901 tcgtggacgc tctgccttga tggttctgct gccggccttt ctggtcctga aggtcttcag
      961 cctgaaaccc gggaatcagt cgactgccgg ctgagcccgc atgggccgct tcacacggac
     1021 tgctcgccag actgacggct ccgcctcgcc cgattcgact acggtgcgca gcctgaagga
     1081 ccgtaccgtt caacccgcta cagcccggca cgctgcagca actctgatgc taggattccc
     1141 aggacaacca acacacacaa caagttcacc agcgtcagga cacgaagcag cctcagcgca
     1201 gcgcttgcga tgtccatcag taccggctag gattcgaaag ttcttacgcg cttctacgtt
     1261 cagggttacg taggggttgt cgggcggttc gccagacatg taacgattga gtgcatttac
     1321 cgctgcgaaa ccggcttcgg gttttcctgc gggcactcgg aaaacccgaa gccggtttcg
     1381 cagcgcattt caggtaagaa catctcaaaa gaatatctgc gatgagggaa gttctttttt
     1441 cgaagatcga tctttccgcc tcacgattga agccctatga gggcttcatt tttttgtgcg
     1501 gcggcccaac gggtgtggcc gcaacccatc ccgtgtccat ccgagatgca atttatcgcg
     1561 aactcgcgaa ggacaaagac atcgatcgtc gcatccgagt cgccgagcac tacaaagatt
     1621 ggtccagcga agctacctat actgaccttg tctctttcga acgtcatttg gctgaactgt
     1681 cgtcggtgat cgtacttgtc ttggagagtg cggggtcaat cgccgagctt ggtctgttct
     1741 ctgtcatcga agagttcaag aaaaagctcc ttgttttcat cgagaccgaa cactacaaga
     1801 ctgactcgtt cattcgtctt ggaccaatcg actatttgga gaagaactgc aacaactccg
     1861 ccgagtgcca tcgttggatg aaaggctcag gacgagctgc ggtttttgat cagattgcgg
     1921 catctgatct acaaactgac cttgctgcgg cagttcgttt cagagcatca ttaccttcac
     1981 cggaacgcca actccatccg tccggatggc ttgatggggc attgctagta tgtgacttgc
     2041 tgaacatctg ttcagcgcta accattcgcg aactaaagca gttgctcgat ggtctagggt
     2101 gtcagagaaa cgagtcggaa gttaagcaat tgctgtttct cctcacccgc atgggcctcc
     2161 tcgctatgga gcccaagggt gatcagcgct tctacgtcag taccgacgct agggaattcg
     2221 tgagattcgg catgcgcgat gccacgtttg acttgatgcg cttccgttcg gacctccttg
     2281 atctctacca acgagcggac aagaagcgat tccgcgcgat acaagaagcg cgagctcgcc
     2341 atgcatagtg acactgacct catccgtcag atagctcggg acatgggcat gttgcctggc
     2401 caccttgctt cactgattcg tacggcgccg cttcgctata aggtcttctt cattgagaag
     2461 aagtcagggg ggcttcgtga ggtggctcaa ccggcccgtg aagtcaaggt cgtgcaaaga
     2521 tggcttgttc gccaccttac tccgcacctc cccgttcatg aggcggcaac tgcctaccga
     2581 gttggcgcat cgattcgaga caacgctgcg agacatgcga gcagtcgcta tatgctcaag
     2641 cttgatttca ccaacttctt tccgtctatt gttcgatccg acatcagcgc acatcttgag
     2701 cgccactgcg gacagctcta tgactcgtct gccagattac tcatcgccca cacttgctgc
     2761 tgggcgcgga aacgaacccc tcccttacgg ctatgcatcg gagcccctat ttcacccctc
     2821 atgtcgaaca ccataatgtt cgattttgat aagcgcctcg cgtcgttcgc agaagcggac
     2881 ggtgttacgt acagcaggta cgcagacgat ttgacttttt cgacggaccg gccccatgtg
     2941 cttgatcggt acgtctcctt ggtgcgcgag ttgcttgatg aaataagcta tccgtcccta
     3001 cagctaaatg acgaaaagac ggttttcgct tctcgttctg caagacgaac ggtaacaggc
     3061 cttgtcatca caccagatgg atcggtatca gtcggaaggg accgcaagcg cttaattcga
     3121 tcgatgtatc accgcggaac gcttggactg ttgtcacctg atgagacaaa ggagctaatc
     3181 gggctgatcg cctttgtgga cagcatcgaa ccgggattct ctcaacgatt gagggcctct
     3241 ggaagcacgt gaggagacaa gaagctctca aggagcggag cgacgtctga ccttcaatcg
     3301 tcgaagtatc gatgcgatgc gtcgtcggtc tcctgaaggt caactccaaa cgctcccggg
     3361 gtaccggtag ccgctccccc tcacccctaa aacaaaacct ccagccccgc ccggttcggt
     3421 tcggaggcgc caccccaggt gacggtatgt ggccagcggc gcaaaccgcc cgcgcggggt
     3481 ccgtttcagg aagcccttcg ggattagagg cagtttgagg gggtggactg tcgtatttcc
     3541 ggcctcagtc ccagggattc accatccgca cacccgtgcc gtagaagtcg cgcacattgc
     3601 gcgtcgcgat cgtgaaaccc ctggcggcgg cgatcgccgc gatggcgcca tcggcgaagg
     3661 cgatcgggtt gccggcggcc ttggctgcgg cgtgaacgga ggcgaaggcc tcggccgctg
     3721 cggtgtcgaa gtccagcaca cggtcttcga acagggggcg cacctgctgg gtcagcgccc
     3781 gatgcagctc cgtgcggcgg cggccctggg gcaaggccgc tacgccggcc agcagctcgg
     3841 cccagctgac ggtggtcagg aacaggctgc cgaccggctg cgcgtccagc caggccagta
     3901 cagcggcgga cggttcaggc ttgagcggct ctgaaacgac gttcgtgtcg aggatgatca
     3961 ttcgaacgtg gccggttcga tcggtgtctt gtcgcgctgc aggttcagtt ccaggtcatc
     4021 caccccgccc agctcctgcc cgatgcgcgc cagtgcggtg cccagccctg ttcgagggcg
     4081 caccgcttcg tccaggatac gccgcacctc ggcctctgtg ctgcgcccgt gcagggtggc
     4141 cagcagcttg agcgcgcggt gggtctcgtc ggacaggttg cggacggtga tggcgggcat
     4201 ggggtgctcc tggatggtcg atgatatcag tgcatccgat ttgatatcaa tggcgattca
     4261 ggatggccac tcctcaccct tcgaacaaat cccccgcccc agccagccct gcccggttcg
     4321 gcggcgccac ccccaggtgg cggtatgcgg ccagcgtggc gatgcgcccg cgcggggtgc
     4381 gttgcaggaa gccctgctgg atcaggtagg gctcgatgac gtcttcgatg gtgccgggct
     4441 cctcgccgat cgcggccgcc acgttgtcca ggccgaccgg gccgccgtcg aagcggtgca
     4501 ccaccgactc cagcagcttg cggtccatca ggtcgaagcc ctgcgggtcg acgtccagca
     4561 tcgccagcgc cttgtcggca atcgccttga tgatgcggcc gtcgcccttg acctcggcgt
     4621 agtcgcgcac gcggcgcagc aggcggttgg cgatgcgggg
//
LOCUS       DAOQEW010000257         4657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7719_length_4657_cov_8.121469, whole genome shotgun sequence.
ACCESSION   DAOQEW010000257 DAOQEW010000000
VERSION     DAOQEW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_4657_cov_8.121469"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..828
                     /locus_tag="PLS35_17950"
     CDS             325..828
                     /locus_tag="PLS35_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20608.1"
                     /translation="MVERLFTKILVPVDFSPCSEEAYRIALSFARSYQAEVLLLHVVD
                     TKSLDALNRLGLAPASEAAKQKKQLHHHARLNARQLLAWDEAKGLSIKRVLADGCPFE
                     EIARTARVEGVDLVVMGSYGGAVGGVEKIFFGTTAEKVVRTAGCPVLTVPLPVKRAKA
                     RSGKSSS"
     gene            841..1269
                     /locus_tag="PLS35_17955"
     CDS             841..1269
                     /locus_tag="PLS35_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNI20609.1"
                     /translation="MGLKQRSAVLSGGRVWLAICFVLALCSQGGAQAEQRIDVAIKDF
                     AFVTKQVPLRLGVATVITITNEDQERHDFGSTMFDGLPTRVESGGIVSYGRGISGVFL
                     DGKKSAVIRFVMERPGRHEFRCSIHQNMKGELLLLNVEAA"
     gene            1273..2211
                     /locus_tag="PLS35_17960"
     CDS             1273..2211
                     /locus_tag="PLS35_17960"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20610.1"
                     /translation="MVPLIKHMLFATDFSACADRAMGYALSLAGAWKANLCVVTVLEL
                     YPGMDPDYTVNKMYLDHLREEANRQLGAVQARAKAASQSVTTRIEVGIPSQALQTVAE
                     STGADLLVVGTHGRTGLDHVLVGSTAERVVRVSPCPVLAVKADKGVADSSAAAPIKRI
                     VVPVDLSTCSLDALEYAVQFAKPLGAAITILHAMEPVAYGLDFSLSHAKEWKEQRDYL
                     EKRLSVLSACVTAHGIQADHVLKPGLPADSIVSHVTQQGFDLMIMGTHGRRGISHVLL
                     GSIAGAMLRHAPCPVLTVLQVNFGADYERVVPLGNS"
     gene            2242..2466
                     /locus_tag="PLS35_17965"
     CDS             2242..2466
                     /locus_tag="PLS35_17965"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNI20611.1"
                     /translation="MKRKEQSRAAAPPSPRAKEQTAVNPSALPDGMWERISQKAYDLW
                     RERGSREGFALEDWLDAEAIVMEEMHESRE"
     gene            2450..3805
                     /locus_tag="PLS35_17970"
     CDS             2450..3805
                     /locus_tag="PLS35_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20612.1"
                     /translation="MNLANERVVGRSAPVNKDLGRLISRLDQLSLSPGFAQQRRIAFE
                     RALQVYVELGSQSPLTPLREEVELADLLLYADFYPEDGQLTLIEQLRDVITEHIPEEE
                     RVWLDPLKHSYLDLVEILPSQAGAARRSLKSLGDGRLYDIPAESVPRDAALGSVLFTR
                     LVREPGDPESRLAVLAGPMLVLSIQDARALYETTGEERREMEVVGGSFELGDWPEFAK
                     RFGHLLLKNFARMRLAALLEAVSDIRYRTETDAPYFYVMALYEHHEFTFISGGMTELE
                     GWAEDAPPEQAVTGSRRKLRRFVRRGQKELVARITVTATELFLECDSRGSLDEIKHTL
                     AATFGYSLHFRGEIAQPPVRQVTQEELSGNEPLTVVVTGEEDRSLVKGFLETVYLEWA
                     DRESPALGGDTPRHTAKTPEGRDRVAALIDDMERCDFGLVRSGIAAFDYNVLRAHVGL
                     C"
     gene            3824..4315
                     /locus_tag="PLS35_17975"
     CDS             3824..4315
                     /locus_tag="PLS35_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNI20613.1"
                     /translation="MAQDRRTMMDGGEGMQEQVERIVLQARAEPPNVLKTLLAIEEHL
                     GHVPAEAIPVIARRLGATTAQIAGVLSYYPDLRVTAPGRHVIRVCMGESCYANGCGRV
                     LRELQDRLRTDVGETTSGGKFTLDTMSCAGNCAVSPTVVIDRDLYGRVLPSQVETMLE
                     RYR"
     gene            4317..>4657
                     /locus_tag="PLS35_17980"
     CDS             4317..>4657
                     /locus_tag="PLS35_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HNI20614.1"
                     /translation="MTSATRLYLSNDTSARAAGAGVLADAWAERPEVQLIRISSRGAF
                     FLEPMVERDSPEGRIAWFNVQPDDLASILSCNGGTPVRAIPFLANQTRFTFANFGETE
                     PLALDEYQARGG"
BASE COUNT          897 a         1261 c         1549 g          950 t
ORIGIN      
        1 cgagcaagag caatgccatc acggtttcgc agactgcttc gtacagtgag tatggcggcg
       61 tcttagatcc ccgcctgaag cggcgacgca tgagatcggt tattcttcgt aaatgcgacg
      121 ggttcggcaa tctgtggcgt tccgctacag atggtggatg gagcctgtag caagacacca
      181 cctcacaggt ctctgtgtcg gggggcggcc catggcatac cgtgattgca ggggaatcct
      241 gatgattctg tgctggtatc ggagccaagc tgatggcatt gggcttgctg atgtgtaata
      301 cagcagaaag ggtggagagg tcggatggtc gaacgtctgt tcacgaaaat tctcgttccg
      361 gtcgatttct cgccatgctc tgaggaggcc tatcggatcg cgctttcctt tgcgcgttcc
      421 tatcaggcgg aggtcctcct gctccacgtc gtcgacacaa aaagtttgga cgccctcaac
      481 cggctgggat tggcccccgc gtcggaagcc gcgaagcaaa aaaagcaact gcatcatcat
      541 gctcggttga acgcccgcca gttactggcc tgggatgagg cgaaggggct atcgatcaag
      601 cgggtgctcg cggacggctg cccgttcgag gagattgcaa ggaccgcgag ggtggaaggg
      661 gttgatttag tcgtgatggg cagttatgga ggagcagtgg gcggggtcga aaagattttc
      721 ttcggcacaa cggcggaaaa agtggtgcgg accgccggct gtcctgtctt gacggtaccc
      781 ttgccggtga agcgggcgaa agcgcgaagc gggaagagtt catcataagg aggcatggtc
      841 atggggctga agcagaggtc agcggttctc agcggagggc gggtctggtt ggcaatctgt
      901 ttcgtgctgg cgctctgttc ccagggtggc gctcaggcgg aacagcggat cgatgtggcg
      961 atcaaggact ttgcgttcgt caccaagcag gtgccgctgc ggctgggggt ggcgacggtg
     1021 atcaccatca cgaatgagga ccaggaacgc cacgatttcg gatcgacgat gttcgatggc
     1081 cttccgacac gggtcgaatc cggcggcatt gtctcttatg gccgggggat cagcggagtg
     1141 ttcctggatg gcaaaaaatc ggccgtcatc cggttcgtga tggagcggcc gggacggcac
     1201 gaatttcgtt gttcgattca tcagaacatg aaaggggaat tgctcctcct gaatgtggag
     1261 gcggcataag ccatggtccc actgatcaag cacatgctgt tcgccaccga cttctcggcc
     1321 tgcgccgatc gggccatggg gtatgctctg agcctggccg gagcctggaa ggctaatctc
     1381 tgcgtcgtga ccgtgctgga gctctatccg gggatggatc cggactatac ggtcaacaag
     1441 atgtacctgg accacttgcg cgaagaggcg aaccgccaat tgggcgcggt tcaggcccgc
     1501 gcgaaggcgg ccagtcagtc agtcacgacg cgcatcgaag tgggaatccc cagtcaagcg
     1561 cttcaaaccg tcgccgagtc gaccggcgcg gatctcctgg tcgtcggcac gcatggccgg
     1621 acggggctgg atcatgtctt ggtgggcagc acggcggagc gcgtcgtgcg ggtctctcct
     1681 tgtcctgtcc tggcggtcaa ggcagacaag ggtgttgccg attcgtctgc ggcggcgcca
     1741 atcaagcgga tcgtggtgcc ggtggatctc tccacctgtt cgctggatgc cttggagtat
     1801 gccgtgcagt ttgccaaacc gcttggggcc gcaatcacca tcctccatgc gatggaaccg
     1861 gttgcctacg gcctggattt cagtttgagc cacgccaagg agtggaagga gcaacgggat
     1921 tatttagaga agcggttgag cgtgttatct gcctgcgtga cggcccacgg aatccaggcc
     1981 gatcatgtgc tgaagccggg gctgccggcc gattcgattg tgtcgcatgt cacccagcag
     2041 ggattcgatt tgatgatcat ggggacgcat gggcgtcggg gcatttccca tgtcctgctc
     2101 ggcagtattg ccggcgcgat gttgcgtcat gcaccttgcc cagtcctgac cgtgctccag
     2161 gtcaactttg gcgcggacta cgagcgggtc gtccccctgg gaaattcatg aatcgaacag
     2221 gttcaccgga aggaggcctc catgaagcgt aaggaacaat ctcgtgcagc tgctcctccc
     2281 tctccccgag ccaaggaaca aaccgccgtc aatcccagtg cgttacccga cggcatgtgg
     2341 gagcgcattt cccagaaggc gtatgacttg tggcgggagc gaggatcccg tgaagggttt
     2401 gcgttggagg attggctgga tgccgaggcc atcgtcatgg aggagatgca tgaatctcgc
     2461 gaatgaacgg gtcgtcgggc ggtcggcgcc tgtcaataaa gacctggggc ggctgatttc
     2521 gaggctcgat cagctctccc tgtccccggg attcgcgcag caacgccgta tcgcttttga
     2581 acgggcgttg caggtctatg tcgaactggg gagccagtcg cccttgacgc cgttgcggga
     2641 ggaggtcgag ctggcggatc tcctgctcta tgccgacttt tatccggaag acggacagct
     2701 cactctcatc gaacaactgc gcgacgtcat cacggaacac atccccgaag aagagcgggt
     2761 ctggctggat ccgctgaaac attcgtacct ggatcttgtg gaaatcctcc cgtcacaggc
     2821 gggagcggcg cgcagaagtc tgaaatctct gggagacggt cggctgtatg acataccggc
     2881 ggagagtgtg ccccgggatg ccgcactggg atcggtgttg ttcacacgtc tggtgcgcga
     2941 accgggcgat ccggaaagcc ggttggcggt gctggccggc ccgatgctgg tcctatccat
     3001 tcaggacgcg cgggcgctgt acgaaacgac cggtgaagaa cggcgcgaga tggaagtggt
     3061 tggaggctca ttcgaactcg gagactggcc ggagtttgcc aagcggttcg gccatctcct
     3121 gctgaagaat tttgcgcgca tgcggttggc cgcgttattg gaagccgtga gcgacattcg
     3181 gtatcggacg gaaactgacg caccgtattt ttatgtcatg gccttgtacg agcatcatga
     3241 attcacattc atttccgggg gcatgaccga gctggagggc tgggcggaag acgcaccgcc
     3301 cgagcaggcc gtgaccggta gccgccggaa acttcgccgt ttcgtccggc gagggcagaa
     3361 ggaactcgtg gcgcgcatca cggtgacggc gacggagctg ttcctggaat gtgactcacg
     3421 gggatccttg gacgagatca agcatacgtt agcggccacc ttcgggtatt cgctgcattt
     3481 tcgaggcgaa attgcccaac cgcctgtacg gcaggtcacc caggaagagc tgtccggtaa
     3541 cgaaccgctg accgtggtgg tgaccggcga agaagatcga tcgctggtga agggtttcct
     3601 tgagacggtg tatctggaat gggcggaccg ggaatctcct gcgttgggcg gcgatactcc
     3661 gcggcatacg gccaaaacgc cggaggggcg cgaccgggtg gccgcgctca tcgacgacat
     3721 ggaacgctgt gatttcggtc tcgtccgcag cggtatcgca gcgttcgact acaacgtctt
     3781 acgagcgcat gtcggcttgt gctgatgtga gcacggagtt cctgtggcgc aagacagacg
     3841 gacgatgatg gacggagggg agggcatgca ggagcaggtc gaaaggattg tgctgcaggc
     3901 tcgcgccgag ccgcccaacg tgctgaagac gcttctggcc attgaagagc acctggggca
     3961 tgtgccggcc gaagcgattc cggtcatcgc gcggcgactt ggcgccacga ccgcgcaaat
     4021 cgccggggtg ctgtcctact atcccgattt gcgggtgacg gccccgggcc gccatgtcat
     4081 tcgcgtctgt atgggagaat cgtgctatgc aaacggttgt ggccgggtct tgcgggagct
     4141 gcaggatcgt cttcgcaccg acgtaggcga aacgacctca ggaggaaagt ttaccctcga
     4201 caccatgtca tgcgccggca actgtgccgt ctctccgacg gtcgtgatcg atcgagacct
     4261 gtacggtcgg gtgttgccat cgcaagtcga gacgatgctg gaacgctatc ggtaacatga
     4321 ccagcgcaac gcgactctac ctttccaatg acacgtcggc cagagctgcg ggggctggtg
     4381 tgttggccga cgcctgggct gaacgccccg aggtgcagct catccgcatc tcttcacggg
     4441 gagcattttt tctcgagccg atggtcgagc gcgacagtcc ggagggtcgg atcgcctggt
     4501 tcaatgttca accggacgat ctggcatcca tactgtcatg caacggcggg acaccggtcc
     4561 gggccattcc ctttctcgcc aatcaaaccc gcttcacttt tgccaacttc ggcgagacag
     4621 aacccttggc tctggatgaa taccaggcgc gcggcgg
//
LOCUS       DAOQEW010000258         4634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7787_length_4634_cov_8.892335, whole genome shotgun sequence.
ACCESSION   DAOQEW010000258 DAOQEW010000000
VERSION     DAOQEW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7787_length_4634_cov_8.892335"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1838
                     /locus_tag="PLS35_17985"
     CDS             333..1838
                     /locus_tag="PLS35_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI20615.1"
                     /translation="MVKLQRYFVVLKEDMNMDFSFDRPGSVKIELKNLNRINVIIGRN
                     GAGKSRFLRALDNEMVINKNNYRVSYVTPERAGIFQRDPGIETNTSNSADWAANTRRA
                     NQASGFKAMSHFALRNAERAYLVKLEDCDARGKSFQRDCLDPISRMLSNVSISRGEVD
                     FVFHSTNGDVVSANQLSSGESETIALASEVLSFLAAIDRTKFNVLLLDEPDVHQHPDL
                     QARFGQYLLEQVSKLDEADREKVVICIATHSTSLVCALASSDLTAVGTKEFDSNVVTL
                     AAPQDNLRKVGPFFGHPLSMSLSNDPMLILEGEDDERVWQQAARTSQGRIRIFPVLAT
                     SVDQQSELESFTDRMLKAIYDEPLAYSLRDGDGTQGPLSKIGCIERFRLQCYAIENTL
                     LTTECLDKMGVATWAEFEVKTAAWISANDGHKAIPHLEALITSSDRLRHVKIKDIRHI
                     IVAITGTKKPWEVVVGQALAGAIGTPCAPADPFGLLTFVGPDAAKQLLQAV"
     gene            complement(2321..3304)
                     /locus_tag="PLS35_17990"
     CDS             complement(2321..3304)
                     /locus_tag="PLS35_17990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HNI20616.1"
                     /translation="MFKGILIERDDAGYRANLRDIDDAQLPDGDVTVRVSHSTLNYKD
                     GLAITGKGPVVRRFPMVPGIDLVGTVESSTHADYQPGDSVLLNGWGVGEGHWGGLAQK
                     ARLKGDWLVPLPNQFSPQQAMAIGTAGYTAMLCVLALERYGVKPEHGEVVVSGAAGGV
                     GSVATAVLTKLGYTVVAISGRSEEADYLKRLGASEVLNRADFSAPGKPLGKERWAGAV
                     DVVGSHTLANICATTKYRGVVTACGLAGGMDFPATVAPFILRGVTLAGIDSVMCPRPD
                     RLEAWRRLGIDLDVSKLEMIGTEISLADAIPTAAKLINGEIRGRVIVDVNR"
     gene            complement(3355..3993)
                     /locus_tag="PLS35_17995"
     CDS             complement(3355..3993)
                     /locus_tag="PLS35_17995"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNI20617.1"
                     /translation="MELQHSPPRKRGRPPKRPEGTLETRELLVRAGLEVLTEKGFAAT
                     GIDEVLKRVGVPKGSFYHYFDSKEAFGAALIDRYASYFARKLERHFIDESMTPLDRLR
                     AFVDDAQAGMARHDFCRGCLIGNLGQEMAALPESFRERLNAVFDDWERRLARCLEAAQ
                     QLGQIPAAVDCTQQASFFWIGWEGAVLRAKLERSAKPLDIFARGFFTGLQLA"
BASE COUNT         1017 a         1283 c         1296 g         1038 t
ORIGIN      
        1 cctggccgct tggcgggcgg cttggcgtgg aaagttgaat atttcagggc cgactagttc
       61 gccgagaagg ggagcgtaag gtagtccgta tgcttcgctt caccgcaacc tgccctctat
      121 gtggtggtga ggttgaggtt caggaggggc gtcgtgctct tcgtggtcgg tatgttggtg
      181 agtgtgggcg taacccaatt gaacacgtgt tcaccttcga tcatgttttg aagtccggac
      241 ggcgagtttc tgtgtaagcc gagaatcctc tgttctgact aaagaagcat tggctacccg
      301 gcatcgccag ggggctctct ccctaagctt tcatggtcaa attacaacgt tattttgttg
      361 tactcaaaga ggatatgaat atggatttct cgtttgatcg cccgggcagt gtcaagattg
      421 aactgaaaaa cctcaaccgg attaacgtga tcatcggtcg caatggagcg ggcaagagtc
      481 ggtttctgcg cgcactggat aatgagatgg tcatcaataa gaataactat cgcgttagct
      541 atgtcactcc ggaacgtgca ggcatatttc agcgtgatcc aggcattgag actaacactt
      601 caaattccgc tgactgggct gccaataccc gacgcgctaa tcaagcctca ggtttcaaag
      661 cgatgtcgca ctttgccctg cgcaatgctg agcgtgcgta cttagtcaaa ctcgaagact
      721 gtgatgcccg gggcaagagc ttccagcgcg actgcttaga tcccattagt cggatgttga
      781 gcaatgtgtc tatttcgcga ggagaggtgg acttcgtttt ccattccacc aatggcgatg
      841 tggtgagtgc caatcaattg agcagtgggg agtcggaaac aatcgcgctc gcatcagagg
      901 ttctgagctt tctggctgcg atagacagga ctaagtttaa cgtcctgctc cttgatgagc
      961 cggatgtcca tcagcatccc gaccttcaag cgcgctttgg acaatatctc ctggagcagg
     1021 tgagcaaact cgatgaggca gatcgcgaga aggtcgtgat ctgcatagcg acgcacagca
     1081 cctctctcgt gtgcgcgctt gcaagcagcg atcttaccgc ggtcggcacc aaggagttcg
     1141 acagtaatgt cgtgaccttg gccgcacccc aggacaatct gcgcaaggtg gggccatttt
     1201 tcgggcaccc gctatcaatg agcctgagca acgatcctat gctgattctc gaaggggaag
     1261 atgatgagcg tgtctggcaa caggctgcac gtacgtcaca aggaaggata cggatatttc
     1321 ctgtgctcgc gacatcagtc gatcagcagt ccgagttgga gtctttcact gatcgcatgc
     1381 taaaagcgat ttacgacgag ccgttggcgt actcgttgcg ggatggcgat ggcacgcagg
     1441 gaccgctttc caagatcggc tgcattgagc gattccggct acagtgttat gccatcgaga
     1501 acaccctctt gacgactgag tgtctagata agatgggtgt cgctacgtgg gctgagtttg
     1561 aggtcaagac cgcagcgtgg atatcggcta atgatggaca caaggcgatc cctcatctgg
     1621 aagcgcttat cacgtcgagc gatcgcttga ggcacgtgaa gattaaggac attcgtcaca
     1681 ttattgtggc catcacaggt accaagaaac cctgggaggt cgtcgttgga caggcattgg
     1741 ctggagctat cggtacaccc tgtgctccag cagatccgtt cggactcctg acttttgttg
     1801 ggccggatgc agccaagcag ctccttcagg cagtctgatt tgggcaaagc agcacttcga
     1861 gctaacgagt ttgccgggaa ttttcactaa ccgtggcacc aagtttggat tttttcacta
     1921 tctacggcac tactgttcaa tggtgatgac actgaagatg aaaaatctta tgtaggctaa
     1981 gagcctattt ttcactttct atgtcatcta acaaggttgt cggttgacac ggttagtgaa
     2041 attcttgccg agatagtgaa atattggtga cagagataat gaaaaaagct cccgagggag
     2101 cttttttcat gggcaagttg gacatggtgg agatgtctgg acttcggcca ttggagtcgt
     2161 tgacgttcgt gagctacaaa ggcctgtaag caccccaatg cggacacacg tgggtcggtc
     2221 gatgccgaaa cccgccgcac agcctgttca cacaatttgg ccagcgccga tcctaagcac
     2281 atgttcaaac tgcctgactg caacggcggg tcaccaacgc ttagcgattg acgtcgacga
     2341 tcacgcggcc gcgaatttca ccgttgatga gcttcgcagc tgtcggaatg gcgtcagcga
     2401 gtgaaatctc ggtgccgatc atctccagct tggaaacatc gagatcgata cccaggcgac
     2461 gccaagcctc aaggcgatcc gggcgcggac acatcacgct gtcgataccg gccaacgtca
     2521 cgccacgcag gatgaacggc gccaccgtcg ccggaaaatc catcccgccg gccagcccgc
     2581 atgcggtcac cacgccgcga tacttggtgg tggcgcaaat gtttgccagg gtatggctgc
     2641 cgacgacatc gaccgcaccg gcccaacgtt ccttgcccag cggcttgccc ggcgccgaga
     2701 aatccgcacg attgagcact tcgctggcgc caagacgttt gaggtaatcg gcctcttcgc
     2761 tccgtccgct gatcgccacc accgtataac cgagcttcgt caggaccgcg gttgccacgc
     2821 tgccaacgcc accggctgcg ccagaaacga cgacttctcc gtgctccggc ttcactccat
     2881 agcgctccag cgccagtacg cacagcatgg cggtgtagcc ggcggtgccg atcgccatgg
     2941 cctgctgcgg cgagaactga ttgggtagcg gcaccagcca gtcgcccttg agacgagcct
     3001 tctgggcgag cccgccccag tgtccttcgc cgacgcccca accgttaagc agcacgctgt
     3061 cgcccggttg gtaatcggcg tgggtgctcg attcgacggt gccgaccaga tcgatgcccg
     3121 gcaccatcgg gaaacgtcga accaccggcc ccttgccggt gatggcaaga ccgtccttgt
     3181 agttgagcgt cgagtgactg acgcggacgg tgacgtcacc gtcgggcaac tgcgcatcat
     3241 caatgtcccg gaggttggcg cggtagccgg cgtcatccct ctcaatcaga atgcccttaa
     3301 acatgttcag ctcctttaca atttagaccg atcgtctatt aaacgttaaa aaaatcaggc
     3361 caattgcagc ccggtgaaaa atccacgtgc aaagatatcg agcggcttgg cactccgttc
     3421 gagtttggcg cgcaagaccg caccttccca gccgatccag aaaaagctgg cttgttgcgt
     3481 gcaatccacc gcggcaggaa tctggccgag ttgttgcgct gcttcgagac aacgggccag
     3541 acgtcgctcc cagtcgtcaa acacggcgtt aagacgctct ctgaagctct ccggcaaggc
     3601 tgccatctcc tgaccgagat tgccgatcag gcaaccgcgg caaaaatcgt gccgcgccat
     3661 tccggcttgt gcgtcgtcga caaacgccct caggcgatca agcggtgtca ttgattcgtc
     3721 aatgaaatgg cgctccagct tgcgggcaaa gtagctagcg taacgatcaa tcagtgcagc
     3781 cccaaaagct tccttgctat cgaagtagtg atagaaggac cccttcggca cgcctacccg
     3841 cttgagcact tcgtcaatgc ccgtcgccgc aaaccccttt tcggtgagta cctccagtcc
     3901 ggcgcgaacc agcagctctc gcgtttcgag tgtgccctcg ggccgctttg gggggcggcc
     3961 gcgtttgcgg ggtggggaat gctgtagttc catgggcaga tattagaccg actgtctcat
     4021 ttaatgcaag cacccctgtc atcgtggctc caaagcgaga agaccgtcgc ggtcatctca
     4081 gaaccagccc ttgtaaacaa cgagactcga accgcagcaa cgaggcgatt gcagtctacc
     4141 cccccttcaa cgtgaccgac cgttttgggt tgagtgcctt ctcccaccga tgacttaagc
     4201 ggtcatttgt ccgcgctcag aaaccgagcg gcagtgagtt ggtgcatagc cgaccgattt
     4261 aggcaggacg cagaaggtca gctccggccg aactgttgtc gttcagggga cacggcttcg
     4321 tgcttcatca tgcagaggca gttcttccgc cagaccaacg acttactatg tctctttgct
     4381 tttgttgtga aagcaacttc ttattaatca acatgttatg tgttgatcaa gcctaggctt
     4441 ctgagtaccc tgtgagttga tccatcaact tcacacgaag aaatgagccc ctttgcccac
     4501 ctgctgcatg acttgcggat gcgtcacggc atccggcagc gtgacttcaa cttcaccgat
     4561 agtgcgcagc gcatggtaga ccctgatgca atcgtggggc ctggaggttg cctgcgtcga
     4621 atccggcgcc cagg
//
LOCUS       DAOQEW010000259         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7923_length_4585_cov_7.089183, whole genome shotgun sequence.
ACCESSION   DAOQEW010000259 DAOQEW010000000
VERSION     DAOQEW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7923_length_4585_cov_7.089183"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..741)
                     /locus_tag="PLS35_18000"
     CDS             complement(334..741)
                     /locus_tag="PLS35_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20618.1"
                     /translation="MTNLRRAVSILLFLSVLLPTAPGWSMDPLPIEPDLNSRLDELYD
                     HESRMFIMLYSLHGDGKVDYVTGRLVQEYTRSNYGNPVYYTEQFPLFYWWNHTMFNDP
                     DQDGVNGNERVYQEDIEFDIARYKPCLFNGQPC"
     gene            899..2230
                     /gene="mtaB"
                     /locus_tag="PLS35_18005"
     CDS             899..2230
                     /gene="mtaB"
                     /locus_tag="PLS35_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNI20619.1"
                     /translation="MPRASLHTLGCRLSQSETAMLGNSLTRQGYRLVEYGEETDLLVL
                     NTCSVTENAEKDCRYAVRKTLRHSPHAFVAVTGCYAQTGAARLQTVPGIDLIVGTQYK
                     MNLPDYLPAPTQLRKQPEPELRHSRTIDREDFVLPGTAYSDSTRALLKVQDGCDFMCS
                     FCLIPFARGRERSRVADDVLREARELAIHGYRELVLTGVNIGQYSYQGMELVDLLQEL
                     EAIPEVVRIRISSIEPTTLPTRLLQHMASSTKLCHYLHLPLQSGDDGILQAMNRRYGI
                     REYEELVEQAQALMPDLGLGTDLMVGFPGEDEQAFANTMAMAARLPFSYFHVFSYSSR
                     PGTPAARLEPQTPGTIIRQRSKTLAELSRTKALAFYQRQIGRTVSVLFEQGEHDGFRT
                     GTTANFTRVAVPADAVAAGSIHPVTITGIMDGLAYGHLASPSVEPTYRPLL"
     gene            2227..3585
                     /gene="miaB"
                     /locus_tag="PLS35_18010"
     CDS             2227..3585
                     /gene="miaB"
                     /locus_tag="PLS35_18010"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNI20620.1"
                     /translation="MTQPNKPHTVHIETFGCQMNEYDSELVRTLLRKAGFEFTEDRER
                     ADVMLMNTCAIRENAHNKVYGHLAELKAVKEQRPLVVGVLGCMAQNLKEELTEKQPLV
                     DVLVGPDGYRQLPGLLTNALNAEEQGLSRRGLAVDLSEYETYDDILPERDGGCNAWIA
                     IMRGCDNFCSFCVVPYTRGRERSRDPQGILREVEVSVEAGHTQITLLGQNVNSYRYDD
                     WDFARLILAVAEVPGVQRVRFTSPHPKDFPAALLDAVAGHPNICKHIHLPLQSGNDRI
                     LELMNRTYSRKEYLDLATTIRRRHPGIALTTDIICGFCSETEEEFLDTFRVVEDVQYH
                     SAYVFKYSERKNTIAARKFPDDIPEAVKGERVSRLVDLQRPVTARINRELIGQTLSVM
                     VEGDSKRSADQWMGRTDTGIYVIWNKSDAPALLGSLQPVTILDGSAAVLMGRGEPGAT
                     LS"
     gene            4048..4179
                     /locus_tag="PLS35_18015"
     CDS             4048..4179
                     /locus_tag="PLS35_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20621.1"
                     /translation="MKKDIIQTHVEEAPKGISLETIIAVGVFGWMALGVVMMGLGIW"
     gene            4266..>4585
                     /locus_tag="PLS35_18020"
     CDS             4266..>4585
                     /locus_tag="PLS35_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20622.1"
                     /translation="MLIHGSHSFLMHRLNSGILGLTLMGALASGCSSTAASVQQPAAM
                     QPQGSVRLEEVADWEFEASHPSSIDAATLDSLLRAVVIAEAQTDMSNLPVDGSKPMTV
                     FSDE"
BASE COUNT          978 a         1386 c         1278 g          943 t
ORIGIN      
        1 tcgtctgcgc ccagttcgac agcgtgaggc tcgtgacgag gcggttcgcc agatcacgag
       61 tcatagtcag cacgctatct cgatcgctca cattggtcaa gttgccgacg agatcgtagg
      121 ctgtctggca gactctcact gatgagctga ttgacggcat cgtaggtgag gagcaggtgg
      181 aggtgacaag agaccggtgt gcaggccagg ctactcagca aggccgtcca gcaaggccgc
      241 agggagtacg acgactgagg cctaccctct gggtacgtcg caaggaggcg tactaccgag
      301 aacgccgctg gcgggtttac tgggcagcct ggattagcag ggttggccgt tgaagagaca
      361 aggcttatac cgggcgatgt cgaactcgat gtcctcctgg tagacccgct cattcccatt
      421 cacgccgtcc tgatcaggat cgttgaacat ggtatggttc caccaataga acaagggaaa
      481 ttgctcggta tagtagaccg ggttgccgta attgcttctc gtatactcct gcaccagccg
      541 tcccgtcaca tagtcaacct tgccgtcacc atgcaaggaa tagagcatga tgaacatcct
      601 cgactcgtga tcgtagagct catcgagacg gctgttcaga tccggctcga tcgggagggg
      661 atccatggac caaccgggag cagttggaag caacacggag agaaacagga ggatactcac
      721 ggctctgcgg aggttcgtca tagtacagaa atccgactga caccacagcc aacacaacgt
      781 gcggtcatgc taccacccgt atccagaccg ttcaagcgct ggcccgactt tttcgcgcca
      841 cggtcacctt gctgatctga tgcgcctcct cttataatcg cgcacgatgt cttccaccat
      901 gcctcgcgca tcactccata cattgggctg ccggctcagc caatcggaga ccgccatgtt
      961 aggcaatagc ctgacgcgcc agggctaccg gttggtggag tatggcgagg aaaccgatct
     1021 gctcgttctc aatacctgct cggtgacgga aaacgccgaa aaagactgcc gctacgcggt
     1081 ccgcaaaacg ttgcgtcatt cgccccatgc cttcgtggct gtgaccggtt gttatgccca
     1141 gacgggcgcc gcgcgattgc agaccgttcc cggcatcgac ctgattgtcg gcacccagta
     1201 caagatgaat ctgccggatt atcttccggc tccgacgcag ttgcgaaaac aaccggaacc
     1261 cgagctccgt catagccgga cgatcgatcg cgaggacttc gtcctgccgg gtaccgccta
     1321 ttccgattcc acccgggcgc tgctcaaggt tcaggacggc tgcgacttca tgtgcagctt
     1381 ttgtctgatt ccgtttgcgc gcgggcggga gcgcagccgg gtcgccgacg atgtcttgcg
     1441 ggaagcgcgt gagcttgcca tccacggcta tcgcgaactg gtgctgaccg gtgtgaacat
     1501 cggccaatat tcctatcaag ggatggaact ggtcgatctc ctgcaagaac tcgaagccat
     1561 tccggaagtc gtccggatca ggatttcgtc catcgaaccg accacgttgc ctacccggct
     1621 gctgcaacac atggcctcct cgacgaagct ctgccactat ctgcaccttc ctctccagag
     1681 cggcgacgac gggattttac aggccatgaa ccggcgctac ggcatacgtg agtacgagga
     1741 attggtcgag caggctcaag ccctgatgcc ggatctgggt ttgggtaccg acctaatggt
     1801 cgggtttccg ggtgaggatg aacaggcgtt tgcgaacacc atggccatgg ccgcacggct
     1861 gcccttctcc tacttccatg tgttcagtta ttcatcccgt cccggcacgc cggcggcccg
     1921 cctggaacct cagacgccgg gcaccatcat ccggcaacgg agtaaaaccc tcgcagagtt
     1981 gtctcgcacc aaggcgctgg cgttttatca gcggcagatc ggccgtaccg tctccgtgtt
     2041 gttcgaacag ggggagcacg acggctttcg aaccggcacc acggcgaact tcactcgagt
     2101 ggccgtcccg gccgatgcgg tcgcggccgg ctctatccac cctgtcacca ttaccggcat
     2161 catggatggc ttggcctatg gtcatctggc ctctccgtcc gtggagccga catatagacc
     2221 attgttatga cacaacccaa caagccccat accgtccata tcgaaacctt cggctgccag
     2281 atgaacgagt acgactctga actcgttcgg acgctgcttc gtaaagcggg atttgaattc
     2341 accgaggatc gcgagcgcgc cgatgtgatg ctcatgaaca cctgcgccat tcgtgaaaac
     2401 gcccacaata aggtctatgg gcatcttgcg gaactcaagg ccgtgaagga acaacgcccg
     2461 ctggtcgtgg gcgtgctggg ctgtatggcg caaaacctca aagaagaact cacggaaaaa
     2521 cagccgctcg tagacgtgtt ggtggggccg gatggatacc ggcagttgcc gggccttctg
     2581 acgaatgccc tcaatgccga agagcaggga ctctcgcgcc gtgggctggc cgtcgatctg
     2641 tctgaatacg aaacctacga tgacatcctt cccgagcgcg acggcggctg caatgcctgg
     2701 atcgccatca tgcggggctg cgacaatttc tgcagtttct gcgtcgtgcc ctacacgcgc
     2761 ggacgcgagc gttcgcgcga tccgcagggt atcctgcgcg aagtggaggt ctcggtggag
     2821 gccggccaca cccagattac gctgctcggc cagaacgtga actcctaccg gtatgacgat
     2881 tgggacttcg cccgcctgat tctcgcggtg gccgaagtac cgggtgtcca gcgagtccgg
     2941 tttacctccc cccatcccaa agattttcct gccgcgctct tggatgctgt ggccggtcat
     3001 ccgaacatct gcaagcacat tcatctgccg ttacagtccg gcaacgaccg tattctcgaa
     3061 ctgatgaacc ggacctacag ccggaaggaa tatctcgacc tggccaccac aatccgccgt
     3121 cggcaccccg gtatcgccct gacgacggat attatctgcg gcttctgctc ggaaaccgaa
     3181 gaggagttcc tggatacgtt tcgagtcgtg gaggacgtgc aatatcactc cgcctatgtc
     3241 ttcaaatatt cggagcgaaa aaataccatt gcggcgcgga aattcccgga cgatataccg
     3301 gaagccgtca aaggtgaacg agtcagccgc ctggtagacc ttcaacgtcc cgtgaccgcc
     3361 cgcatcaacc gggagttgat cggccagacc ctctccgtga tggtggaagg cgactcaaaa
     3421 aggtcggcgg atcaatggat gggccgcacc gataccggca tttacgtcat ttggaacaag
     3481 agcgacgctc cggccttgct tggaagcctt caaccagtca ccattctcga tggaagcgcc
     3541 gccgtcctca tgggccgggg cgagcctggt gccactctct cgtgattcat ccctcagttg
     3601 ttcacaatcg aatgacccat caccggtcat tgcaccggac gatcggctcc cctcgcaatg
     3661 tggaccaatc gtcctgtgca gaaagaggag gatcttattg cccagttctg aacaattctt
     3721 gccaaatttt agacgcccgc gcgcgtcaga ctgacaaaaa taaggaacgc agatacgacg
     3781 tagcgagttc gtattttcca accttctccc gagcccatca cttcttacct ctcaacggag
     3841 ccttttcact gccttgaaac atcaatagct taggccccag tgttgagcgt gtgccgccac
     3901 gtcttcacct caacatacat tacagacagc gattgaacgg gagtgagaaa tcgcacttcc
     3961 gaagtctttg gaaacagggc atagcctttg cttggtccag tctggcaacc acaccaacca
     4021 cccgagcgag ccagaggacg ccgcatcatg aagaaagaca tcatccagac ccacgtagaa
     4081 gaagccccta aggggatcag cctggaaacc atcatcgcgg ttggcgtctt cgggtggatg
     4141 gctctcggcg tggtgatgat gggtctgggg atctggtaat cccgatccgt cgtttcgcct
     4201 gttctctctg aaaccgcacc gcctctgttc gtcaccgctt cgaccagccg agaaggaaga
     4261 ccgccatgct gattcacggc tcccactcct ttttgatgca ccgcctgaac tccggaatcc
     4321 tcggattgac cttgatggga gccctggcct ccggttgttc ctccaccgcc gcctcggttc
     4381 agcaaccagc cgccatgcag cctcaaggct ccgtccggct ggaagaagtc gccgattggg
     4441 aatttgaagc ctcccatccc agctccatag atgccgccac actcgacagc ctgttgcggg
     4501 ccgtcgtgat tgcggaggcg cagactgata tgtcgaatct gcccgtggat ggcagcaagc
     4561 cgatgacagt gttcagcgat gagga
//
LOCUS       DAOQEW010000260         4580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7937_length_4580_cov_7.488619, whole genome shotgun sequence.
ACCESSION   DAOQEW010000260 DAOQEW010000000
VERSION     DAOQEW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_4580_cov_7.488619"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1130)
                     /locus_tag="PLS35_18025"
     CDS             complement(393..1130)
                     /locus_tag="PLS35_18025"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNI20623.1"
                     /translation="MKKRSLTINGLIPALWLLCVVMAAPLGHAREPVPNYDPQGYVSD
                     HAGVIEADWRARIRSVCQDLERKTGVEMVVVTVPTLKPYPSANDYAMGLYQKWGIGSA
                     QDEHGVLILLAVQERQAAVTMGRRMIPVMGGEVVGKVGHEYLDPAIKNGHFDEGLYRT
                     VVALASVSQEIRVGSTKKAHFRGLGFWITVTTASGALWFLWWLSRPDLRHPFGRIRKG
                     QYWGTGQGGFGGNFGGMGGGMSGEGWK"
     gene            complement(1127..1885)
                     /locus_tag="PLS35_18030"
     CDS             complement(1127..1885)
                     /locus_tag="PLS35_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20624.1"
                     /translation="MTQPTALDVPSEVQQLLQRYVKETTTLLGPQLEGILLYGSAVGG
                     EFLPGRSNLNLLLMLSGYDREGMKRYAKAHKRWSREQFVVPLLVTETELTKPGTVFPL
                     EYLEIQEQHRVLWGKDPFIGLHIDRTHLAYQVQQGIQGNLVRLRQRFIEGGGTEEATT
                     MLLPLSLTALLPCLRGLQRLAGHPALFQSDALLTAIRHMTGLDLAGLGDVLELKRGII
                     SPGPVEVPRLYERYAGNLEALIALIRPDGTVAPR"
     gene            complement(2088..3365)
                     /locus_tag="PLS35_18035"
     CDS             complement(2088..3365)
                     /locus_tag="PLS35_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNI20625.1"
                     /translation="MARASIETDVAIVGAGGGGAVLALMLAQQGVRSVVLERASGPPQ
                     GLRGEILQPNGQRVLDRLGLLDKLPAHAVRSVHRFNFCRSGGERLCTVDYSDLPAPYN
                     RAVVTLPNVAHHAILDALEKQNPGGLWYDATFTGLRFDGSRVVGLQATRHGEPVEVSS
                     RLVVGADGAFSKVRESLGISADIHRYPESYLIAILKAPTGFDEARYLVGKREILGLFP
                     AAGQQVYAFYMIKAGSYDAVKAQGLESLRRAWVRIDPAMESIVSGLVDWNQTGYMPTG
                     RVRTDRWVADGAVLIGDAAHAMNPHASQGRMQAMVDAVTVADLIPGWLKNNTFSSEAL
                     RAFEVARRPQVSMLQRLADEQCRFWNTGNPLLAFLRDRVFRTLDRNARLRYRVLTTTA
                     GLRSQPPFSMLDRVMAAGLLPDPSADRKSSVGT"
     gene            complement(3374..3919)
                     /locus_tag="PLS35_18040"
     CDS             complement(3374..3919)
                     /locus_tag="PLS35_18040"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNI20626.1"
                     /translation="MSHPTSLPLMALIVAGFMFLSQGPVCASATIEVYYAPEDHPIDH
                     VVNLYGRATRYIYVSVYGLTAPSIVKALVEAKHRGVDVRVITDRERLTDPKQHSAVKT
                     LRLAGVPVRINRHDALMHLKQVVIDDEINTSGSANHTTSGNRYNDERLDVITDARLTA
                     RAREKFLAMWNDRERFVVWTE"
     gene            complement(4134..4514)
                     /locus_tag="PLS35_18045"
     CDS             complement(4134..4514)
                     /locus_tag="PLS35_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20627.1"
                     /translation="MWKKNFLFRAHEAAPLKESENELFHDDAEPALDSAGLQMEKFLS
                     VWVQGEGEDEKPTMYTNMYVRTATLDFKTRAGFLQPLQGRTHQIKQMLTPEQKGFLRE
                     WLSTTSPQAWEDSDDHFRTLFDLE"
BASE COUNT          972 a         1435 c         1255 g          918 t
ORIGIN      
        1 atccatccgc gatgatgaca caactgcagg gataaagcga cagctagccg tctcttcatt
       61 catgtcgcat caagcctgag gtggactgga ttaggccccc actgcgcgcc tccaacggga
      121 gccttcttaa gccgtgcgtt gcgcgaggtg cgcgatgcga cgaataacga gtgttacgtt
      181 tgcgcacgcc gcctaggcgg tgagccggcc gtgtcttcca ggaggctaac gggccgcccg
      241 ccgctccgag tgcacttcga caatatttct tccatgctcg cttcgcttca caagagggtg
      301 gcacgcttgg tctcccagtg cgcgcgtcca acgaggggag tcttcgaccg cgcgttgcgc
      361 gagcaaggga gaccaacggg ccaccctcct ccctacttcc acccctcccc gctcatcccg
      421 ccacccatgc cgccgaagtt gccgccgaat ccaccctgtc cggtccccca gtattgtccc
      481 tttcggatgc ggccaaaggg gtgccgcaaa tccgggcggc tcagccacca caaaaaccag
      541 agggctccgc tcgccgtggt gacggtgatc cagaagccca gtccgcgaaa atgcgccttc
      601 ttcgtggagc cgacgcggat ctcctgcgag acagaggcca aggcgactac cgtgcgatac
      661 agcccttcgt cgaaatgccc gttcttaatc gcggggtcga gatactcatg tccgaccttg
      721 ccgaccacct ccccgcccat caccgggatc atgcgccgcc ccatggtcac ggccgcctgg
      781 cgttcctgca cggccaacag gatcaacacc ccatgttcat cctgggcgga gccgatgccc
      841 catttttgat agaggcccat cgcataatcg ttggctgagg gatagggctt cagcgtcggg
      901 acagtcacca ccaccatttc cacgccggtc tttcgttcaa ggtcctggca gacggaccga
      961 attctggcgc gccagtccgc ttcgatgacg ccggcatggt cactgacata cccttgggga
     1021 tcatagttcg gaaccggttc ccgcgcatgg cccaacggtg ccgccatcac tacacacagc
     1081 agccagagcg ccgggatgag tccgttgatc gtgagtgagc gcttcttcat cgcggcgcca
     1141 ccgtgccatc cggcctgatc aaggcgatca aggcttcgag atttccggca tagcgctcgt
     1201 ataagcgcgg gacttccacc ggtccgggcg aaatgatccc gcgcttcaat tccaacacat
     1261 caccgagtcc tgctaaatcg agtcccgtca tgtggcgaat ggccgtgagc aacgcgtccg
     1321 attgaaataa cgccgggtgt ccggccaggc gctgcaaccc gcgcaaacag ggcaataagg
     1381 ccgtcaacga cagcggcaat aacatcgtcg tcgcttcctc cgtcccgccg ccctcgataa
     1441 accgctgccg caatcgcacc agattgccct gaataccctg ctgtacctga tacgctaaat
     1501 gtgtgcgatc gatatggagg ccgataaacg gatccttccc ccagagcacg cgatgttgct
     1561 cctgtatttc cagatattcg agtgggaaaa cggtgccggg tttggtcaat tcggtctccg
     1621 tcaccaggag cggcacgacg aattgttcac ggctccagcg cttatgggct ttggcgtacc
     1681 gtttcatccc ttcccggtca tagccggaaa gcatcagcaa aagattgaga tttgaacgac
     1741 ccggcaaaaa ctccccgcct accgcgctcc cgtacaggag aatcccctcc agttgcggac
     1801 cgagcagagt ggtcgtttcc ttgacgtatc gctgcaggag ttgttgcacc tcggagggga
     1861 catcgagcgc ggtcggttga gtcacaaaca cctcggctgt agttgggtag gatgctgaaa
     1921 agtcttccag tggcgctctc gcctcgttcg gagcctcaac gtaccacaag ggtacgcctc
     1981 agctcttcgc ttggctgcgg ccttgctgga cgactttttg aacatgcccc gggacaattc
     2041 attcttactc tgatgaaact ctcgtggcct acaaggacca tcttcttcta tgttccgacc
     2101 gatgatttcc gatccgcaga ggggtctggc aacaaccccg cagccatcac gcgatcgagc
     2161 atcgaaaacg gaggctggct tcgcagtccg gcggtggtcg tcagcacacg ataccggagg
     2221 cgcgcattac ggtcgagcgt acgaaacact cgatcgcgca ggaaggcaag cagcgggttc
     2281 ccggtgttcc agaatcgaca ctgctcgtct gccagacgtt gcaacatgct cacctgcggc
     2341 cgccgggcaa cttcgaaggc gcgcaacgct tcactcgaaa acgtattgtt cttgagccag
     2401 ccgggaatga gatcggccac cgtcaccgca tccaccatcg cctgcatgcg gccttgagat
     2461 gcatggggat tcatggcatg agccgcatcg ccgatgagca ccgcgccatc tgccacccac
     2521 cgatcggttc gaacacgccc ggtcggcata tagccggtct gattccaatc gaccaaacct
     2581 gagacgatgg attccatggc agggtcgatc cggacccaag cccgtcgtaa cgactcaagt
     2641 ccttgagcct tcacggcgtc atacgatccg gccttgatca tatagaatgc gtagacctgt
     2701 tggccggcag cgggaaatag tcccagaatt tcacgtttgc ccaccagata ccgtgcctca
     2761 tcgaaccccg tcggagcttt gagtatggca atgagataac tttccggata ccgatgaatg
     2821 tccgccgaga taccgagtga ctcgcgaacc ttggagaatg caccgtcggc cccgactacc
     2881 aaacgcgaag acacctcaac cggctcccca tgacgggtcg cctgcagacc gacgacacgg
     2941 ctgccgtcga aacgaagccc ggtaaaggtg gcatcgtacc agagcccgcc gggattctgc
     3001 ttttccaacg catcgaggat cgcatgatgc gcgacattgg gcagcgtcac cacagcgcga
     3061 ttgtaaggag ccggaaggtc actgtaatcg accgtgcaca gccgttcgcc gccggatcga
     3121 cagaagttga atcgatgcac cgatcgcacg gcatgcgccg gcagcttgtc gagcaaccct
     3181 agacgatcga gcacccgctg gccattgggc tgcagaattt ccccgcgcag accttgcggc
     3241 ggaccagaag cccgttccaa caccacggag cgtaccccct gttgcgcgag catcaatgcc
     3301 aacacggcgc ctccgccgcc cgcgccgaca atcgctacat ctgtctctat gctggcacgt
     3361 gccatgccac tccttactct gtccagacga cgaatcgctc gcggtcattc cacatggcca
     3421 aaaatttctc gcgcgctctg gccgtcaacc gggcatcggt gatgacatcg agtcgctcat
     3481 cattgtaccg gttgccgctc gtcgtgtggt tcgccgaccc ggaggtattg atctcgtcgt
     3541 cgatcaccac ctgcttgaga tgcatcaagg catcatgccg gttgatccgg acgggcacgc
     3601 ccgcgagtcg caatgtcttc acggcgctat gctgcttcgg atcagtgaga cgttctcgat
     3661 cggtaatcac tcgcacatcg acgccgcgat gtttcgcctc caccagtgct ttcacgatag
     3721 agggagccgt cagcccgtac accgagacgt aaatgtatct ggtcgcccgg ccatacagat
     3781 tcaccacatg atcaatgggg tgatcttcgg gagcatagta gacctcgatc gtggcggagg
     3841 cacagaccgg tccctgcgac agaaacatga acccggccac aatcagggcc atcagaggaa
     3901 gagatgtggg atgactcatg cgtggtctag ctggatgaag agagacggta cacactcgcg
     3961 cactgtgatc ccttgcatat gaaccataga aagatttcca caggaggttc aaaacgttcg
     4021 tccagcaaag ccgcagcgaa tgaagatccg gagcgtaccc ttgcggtacg ttgaggatct
     4081 gaatgatgag ggaacgccgc tggcggaggt cttcagcatc ctgattgaat ggatcactcg
     4141 agatcaaaga gggttcgaaa atggtcgtcg gagtcttccc aggcttgagg tgaggtcgtg
     4201 gacaaccact cgcgtagaaa tcccttctgc tccggcgtca acatctgctt gatctgatgt
     4261 gtgcgtccct gcagaggctg gagaaatccc gcccgggttt taaaatcgag cgtagccgta
     4321 cgcacataca tgttcgtata catggtgggc ttttcgtctt cgccttctcc ctgcacccac
     4381 accgagagaa acttttccat ttgtaggccg gcgctgtcca aggccggttc agcgtcgtca
     4441 tggaacagct cgttttccga ttctttgagc ggagccgctt catgcgcacg gaataagaaa
     4501 tttttcttcc acatacccta ccctccagac cggcgcatac tgtaagctag gcatggtgaa
     4561 agtcaagaaa gcggccgtct
//
LOCUS       DAOQEW010000261         4578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_7942_length_4578_cov_8.246960, whole genome shotgun sequence.
ACCESSION   DAOQEW010000261 DAOQEW010000000
VERSION     DAOQEW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7942_length_4578_cov_8.246960"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..302
                     /locus_tag="PLS35_18050"
     CDS             33..302
                     /locus_tag="PLS35_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20628.1"
                     /translation="MNATTTRTLPLTGKTRSTPAVSQHDTCRCLRCGGFLVDERCMDI
                     GESLGGYWFMAMRCVQCGDITDEVILRNRSAQLATTTQHEVVRAA"
     gene            complement(392..763)
                     /locus_tag="PLS35_18055"
     CDS             complement(392..763)
                     /locus_tag="PLS35_18055"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HNI20629.1"
                     /translation="MEPIGRRRWVIAEGYIPSKSHGPEPQMTSHETMCILNSSDQDAT
                     VQVTIFYADREPVGPYIIQVPARRTKHVRFNDLTDPAPIPLDTDFSSLIDSNVRIVVQ
                     HTRLDSRQAENALFSTMAFPV"
     gene            complement(801..2552)
                     /locus_tag="PLS35_18060"
     CDS             complement(801..2552)
                     /locus_tag="PLS35_18060"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNI20630.1"
                     /translation="MATTVADLLVERLIQWGVDTIFSLPGDGINGIYEALRTRQDKIR
                     VVLVRHEESAAFAACGYAKFTRRLGVCLATSGPGGIHLLNGLYDAKCDGQPVLAITGH
                     TYHDLIGTHYQQDVDLERLCSDVAAYSQRVMSPAHVGNVVDEAIKTAISRRTVAHITI
                     PKDIQDWSVEDRGSKANIPGHSGDLYSDPLPLPSRSLLEKAATLINAGSKIAILAGRG
                     CLGARAEVLQLAELLGAPIVKPLLGKAVVPDDHPLTTGGIGLLGTAPSQEVLEACDTL
                     IIAGTSFPYLEFYPKPGQARAVQIDLDAARIGLRYPIEVGLVGHCWDVLRALLPLLEK
                     KSDRSFLNTAQASMQQWNELMEERGTRTDTPLKPQVVVRGLNEFLADDAIICCDTGTV
                     TTWAARHIIIRENMEFSASGTLASMGNGLPYSLGAGIAYPGRQIVCLAGDGGCTMLMG
                     ELATLVRYGLPVKIIVLKNNLLGMIKWEQLAFEGNPQYGVDLQPIDFALFAKACGATG
                     LTVDDPGDVREVLREAFAVQGPAVVQAVIDPLEPPLPGKITTTQAWQFAKALAKGQDD
                     RWGIIKTLVESKIREVV"
     gene            complement(2571..3752)
                     /locus_tag="PLS35_18065"
     CDS             complement(2571..3752)
                     /locus_tag="PLS35_18065"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNI20631.1"
                     /translation="MKPGYVETLAEERPRVQTSKERGTSVANRHLWEITPVRDLLWLG
                     GILFLLWFGYYLRGVFTPVLVALLLAYLVNPLVRRAETAWHIPRPVTVSIVLLLSAMM
                     LGGLVTWLGPLLAEQVQSFAERVPGYLQSIATQYHVTLGNFSEPLSSIATSLRDDPLS
                     ILKPIFSGTGQAFGVLGSVIGTTADVTLALVLIPIYFFFFAWQFDSALAQLRRYIPPG
                     YRPRARHIVRRMDQAVSGFFRGRLTIALASAVLYSVGWALTGIRYWFILGLITGILTI
                     IPYASLIGWPLAVLLKYLDGFSAGAGSFDVMTVVVWPSVAYLVVQFIESWLLTPWVQS
                     QSMEMNAVTVLIVVFIGGALGGFYGLLLAIPIAACLKILVQELVLPRLEHNAASPPPS
                     F"
     gene            complement(3795..>4578)
                     /locus_tag="PLS35_18070"
     CDS             complement(3795..>4578)
                     /locus_tag="PLS35_18070"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNI20632.1"
                     /translation="LAYYFLLALFPALLFLTALLGLFPLEQVLPELMTYLRTVLPADA
                     LSLLQKYLDSVVKGSGSDILSLGLLGALWASSSGVTAIMESLNVVYGAQETRPYWKVR
                     LVAALLTIGLAAFIIISITLVLYGERIGEWIADLIGLGWLFLLTWNVLQWPVAIGLML
                     LALAIIYAVCPDVDHDWRWVTPGSVVAVLLWMGLSLGFKAYVDHFGNYNAAYGSIAGV
                     IVLMLWLYLTGVVILLGGEINAQIERAALALRSFSEQSSHTA"
BASE COUNT         1040 a         1336 c         1318 g          884 t
ORIGIN      
        1 gcctatcagg atcacgacga aaggagaacc gtatgaatgc caccacgacg agaacccttc
       61 ctctcacggg caaaaccaga tctactcctg ctgtttccca acacgatacc tgccgatgcc
      121 tccgctgcgg cggattcctg gtcgatgaac gctgtatgga tatcggggag agccttggtg
      181 gttactggtt tatggcgatg cgttgcgtac agtgtggcga catcaccgat gaagtcattt
      241 tgcggaaccg gtctgcgcaa ctggcgacga cgactcagca cgaggtggtg agagccgcct
      301 gacggtcaat cggtagaggg ttccctggaa gagtagttgc ttcttgaagt ctgggcgaga
      361 acgggtttcg cacgttcctg tgcggggcgg ctcagacagg aaaggccatc gtgctgaaga
      421 gcgcattttc agcctgccgg gaatccagcc tggtatgttg cacgacgatg cggacgttgg
      481 aatcgatcag gctggagaaa tccgtatcca gaggaatcgg ggctggatca gtcaaatcgt
      541 taaagcggac gtgttttgtt cggcgggcgg gaacctgaat aatataggga ccgaccggtt
      601 cgcgatccgc ataaaaaatc gtgacctgca cggtcgcatc ctggtccgaa gagttgagga
      661 tgcacatggt ttcgtggctc gtcatttgtg gttccggccc gtggcttttg gatggaatat
      721 atccttccgc aatcacccat cttcggcggc cgatcggctc cataggctct ccccgttaag
      781 tgccagtaca cgcggttgct tcacaccact tctctgattt tgctctcgac caacgttttg
      841 atgatgcccc atcggtcatc ctgccctttg gctaaggcct tcgcaaactg ccaggcttgc
      901 gtcgtcgtaa ttttcccggg tagcggtggt tccaacggat caatgaccgc ctggaccacg
      961 gccggccctt gaacggcgaa ggcctctcgc agcacctcac ggacgtcgcc cggatcgtca
     1021 accgtcaatc ccgtggcgcc acaggccttc gcaaagagcg caaagtcgat cggttgaaga
     1081 tcgacgccat attgcggatt gccctcgaac gccagctgtt cccacttgat catgccgagc
     1141 agattatttt tcaggacaat gattttaacc ggcaggccat acctcaccag cgtggccagt
     1201 tcgcccatca acatcgtgca gccaccgtct cccgcgaggc agacgatctg tcggccagga
     1261 tacgcaatac ccgctcccag actatagggg agcccattgc ccatcgacgc gagtgtgccg
     1321 gatgctgaaa attccatgtt ctccctgatg atgatgtgcc gggcagccca ggtggtcacc
     1381 gtgcctgtgt cgcaacagat gatggcgtcg tcggcgagga actcgttgag gcctcgcacc
     1441 acgacttgtg gtttaagagg agtatccgtt ctggtgcctc gctcctccat gagctcattc
     1501 cactgctgca tgctggcttg cgcggtgttg agaaagctgc gatccgattt cttctccagc
     1561 agcggcaata acgcgcgaag gacatcccaa caatgtccga cgagaccgac ctcgatcgga
     1621 taccgcaagc cgatgcgagc ggcatccaaa tcaatctgaa cggctcgcgc ttggcccggt
     1681 ttcggataaa actccagata cggaaagctg gtgccggcga tgatgagggt atcgcaggct
     1741 tcaaggactt cctgagaggg cgccgtaccg aggagcccaa tgccgccggt ggtcaatggg
     1801 tggtcgtccg gcaccacggc cttcccaagg agcggcttga cgatcggcgc gcccagcaat
     1861 tcagccagct gcagcacttc ggctctcgcg cctaagcagc cccgtcctgc gaggatggcg
     1921 attttcgagc cggcattgat gagcgttgcg gccttctcga gcagtgatcg ggaggggagg
     1981 gggagggggt cgctgtacag gtctccgcta tggccgggaa tgttggcctt ggatccgcga
     2041 tcttcgacgc tccagtcctg aatatctttt gggatggtga tatgggccac ggtccgtcgt
     2101 gaaatggccg ttttgatcgc ctcgtccacc acattaccga catgagccgg gctcatgacc
     2161 ctctggctat aggcggcgac atccgagcag agccgctcca gatcgacatc ctgttgatag
     2221 tgcgtgccga tcagatcgtg gtacgtatgc cccgtgatgg cgagcaccgg ctgtccgtca
     2281 catttcgcat cgtacaaccc gttcagaagg tggatacctc ccggtccgga ggttgcgaga
     2341 cacacgccta atcgtctggt gaacttggca tacccgcagg ccgcgaaagc ggccgattct
     2401 tcatgccgga ccaacaccac cctgattttg tcctggcggg ttcgcaaggc ctcatagatg
     2461 ccgttgatgc catcgccggg gagactgaag atggtatcga ctccccattg aatcaagcgt
     2521 tcgaccaaga gatcagccac cgtggttgcc atcgtcgttc ctttcacgga tcaaaaagaa
     2581 ggaggcgggc tcgcggcgtt gtgttccaac cgcggtagca ccaattcctg gacgaggatt
     2641 ttgagacatg cggcaatggg aatcgccaga agcagaccgt aaaatccacc cagtgctccg
     2701 ccgataaaca ccacgatcag cacggtcacc gcgttcattt ccatggattg gctttgcacc
     2761 cagggcgtca gcaaccaact ttcgataaac tgcaccacga ggtaggccac agacggccac
     2821 accaccaccg tcatgacatc gaaggagcca gcgccggcgg aaaacccgtc cagatatttc
     2881 aacagcacgg ccaatggcca gccgattaat gatgcatagg gaatgatcgt gagaatgccg
     2941 gtgatcaggc cgaggataaa ccaatagcgg atgccggtca gcgcccatcc gaccgagtag
     3001 aggacggcag aggcgagggc gatcgtgagt cggccgcgga aaaatccact cacggcctgg
     3061 tccatcctcc gcacgatatg acgcgctcgc ggccgatacc cgggagggat ataccggcgg
     3121 agttgtgcga gggcgctgtc aaactgccag gcgaaaaaaa agaaatagat cggtatcagc
     3181 accagtgcca gggtgacgtc cgccgtggtt ccgatgacgc tgccaagcac tccgaaggcc
     3241 tgccccgtcc cggaaaaaat cggtttcagg atggaaagag ggtcgtcccg aagactcgtg
     3301 gcaatcgacg agagtggttc ggaaaaattc cccagggtga catggtactg cgtggcgata
     3361 ctctggagat acccgggtac ccgttcggca aacgattgta cctgctcggc caatagcggt
     3421 cccagccatg tcaccagccc tcccagcatc atcgctgaaa ggaggaggac gatggacacg
     3481 gtcacgggcc gtgggatatg ccaagcggtc tcagcgcgcc ggaccagagg attcaccaag
     3541 taggcgagca gcaacgcaac cagcacgggg gtaaacacgc cgcgcaggta atagccgaac
     3601 cagagcagga acaggatgcc acccaaccag aggagatccc ggaccggcgt aatttcccac
     3661 aaatggcgat tcgccacaga agtgccccgt tccttagacg tctgcactcg cggtctctcc
     3721 tccgcaagcg tctcgacata gcctggtttc atgaggcgtt cgatcctttc tctcattcgt
     3781 cgaaacggta ggggctaagc tgtgtgactt gattgttccg agaacgaccg cagcgccaac
     3841 gctgcacgct caatctgggc gttgatttcg cctccgagca agatcaccac acccgtcaaa
     3901 tagagccaca gcatgagcac gatgaccccc gcaatggacc catacgcagc gttgtagttg
     3961 ccgaaatgat ccacataggc tttaaagcct aatgacagcc ccatccagag cagtacggcg
     4021 acgacagacc ccggcgtcac ccagcgccag tcatgatcga cgtcgggaca gacggcataa
     4081 atgatggcga gggcgagcag catcaacccg atcgccacag gccactgcag cacattccag
     4141 gtcaagagaa acagccatcc caatcctatg aggtcggcaa tccattcgcc gatccgctcg
     4201 ccgtagagga caagggtgat ggagatgatg ataaatgctg cgagaccgat cgtgagtagt
     4261 gccgccacca gccgaacttt ccagtaaggc cgggtttcct gcgcgccata caccacattg
     4321 agcgattcca tgatggccgt gactccgctc gaggacgccc agagcgctcc caggagtcca
     4381 agcgaaagga tgtcactccc ggagcctttc acgacactat ccaggtactt ttgcagcaat
     4441 gataaggcgt ccgcaggtaa cacggtccgg agataggtca tcaactccgg cagcacctgt
     4501 tccaacggga acaggcccag caatgcagtc aggaacagca gcgccggaaa aagagccagg
     4561 agaaagtaat acgccaat
//
LOCUS       DAOQEW010000262         4552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8041_length_4552_cov_7.881921, whole genome shotgun sequence.
ACCESSION   DAOQEW010000262 DAOQEW010000000
VERSION     DAOQEW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_4552_cov_7.881921"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLS35_18075"
     CDS             <1..749
                     /locus_tag="PLS35_18075"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20633.1"
                     /translation="SQAIFDAVRPLAFALHCRPMDALRRHYGLPPLGPDLRRVYTDAD
                     LDLFCDLPEFYNAPPQIPGGAFIGPVQWSPPVSLPAWWAQIDGSRPIVYLTLGSSGDP
                     GLLPRLVGALSRLDITLLVASAGAPVGPARDNVLHADYLPGDCVVDRADLVVCNGGSP
                     TCGQAFAAGVPVLGIPGNLDQFLNMYYVERKGLGLTLRSTQLPDAVPTACHRILEDTA
                     YRESARGVALLMERSSTAVRLREALAPLLA"
     gene            complement(850..1638)
                     /locus_tag="PLS35_18080"
     CDS             complement(850..1638)
                     /locus_tag="PLS35_18080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI20634.1"
                     /translation="MREFAGEVFTVWRCGRCHSLHVLESIDYDRYYRNYPIKRQQYDG
                     FTRLTCSKRIKLLSGVGLTKGATLLDHGCGSGHFVRYARAQGILAEGYDPYSSEFGDA
                     SVLARQYQFVTSQDVIEHVDNPSAVIDELKRYVAPGGYLVIGTPNADGLDLHDELDQN
                     AIHAPYHRHIYAAAELQRDVARNDWEVVDFRKEWYVDTWVPFVNSAFLFRYFQSGGGF
                     MDIGFDPVSPMHFIKHPSLLFWGFFGRLFPSQCDMLVVARKRVR"
     assembly_gap    1718..1817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1818..2731)
                     /locus_tag="PLS35_18085"
     CDS             complement(<1818..2731)
                     /locus_tag="PLS35_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20635.1"
                     /translation="MFKKSLLAAALALAAAGAQASVVVTIDYDGAGADAAIKTSSLDW
                     SPDSALITPVTPGDSVNSPYVGQLLQTYVQGTLGSVRNNLGKTVTSPGLNNTYEWTFV
                     GAFREQVSAISIDPTDPLNGPASLSLQSVTGGTNYFEVWVSAVDSNTLAGTGYNNGIK
                     ILSGTIDPYNSSTGAGDTKFTSDPKSNVVALDQANGNNDYPTLYTTTGSGSGDVNLTI
                     GYIDTNYFTGVSVGDAMTIFLQTNLGLPFGQVDPSRLVAGQLAADSSVIGTINGLNGN
                     SLLLQSDAASNFDLKRIPEPTSVALASLG"
     gene            complement(3119..4360)
                     /locus_tag="PLS35_18090"
     CDS             complement(3119..4360)
                     /locus_tag="PLS35_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20636.1"
                     /translation="MLKNFLPSATDMVIAFRNVTRHRRRTLSALAIIVGGVVAFLMTG
                     GFVRWMLENMAEGIIYSEFGHLQIVRPGYFDKGISDPYRFLLPEDAKMDPDIRTVPEV
                     AVVSPRLAFTGLLGYNDATLTFSGAGVDPVDDLQVSKYVTILSGERLSREDPNGVALG
                     EGLAQNLGVKVGDALVLMTNTAKGQLSASDVHVRGIFSTFNKEYDAGALRAPISLVRK
                     LVKVKGATSWVVLVDKTPRTDETLAVMRARLAGRDYQVYSWFDLADYYRKTVDLFEQQ
                     VLVIEIMIGLIVAVSIGNTMQMVVIERTTEIGTMMAIGRRRSDVLRLFLLEGCVLGFL
                     GGLIGAGGGWLLSIAVSKIGIPLPPPPGMSEGFDAEVLVDLPLAINGFVLALGTTMAA
                     SVFPAFKASRTNVVDALRRQA"
BASE COUNT         1006 a         1376 c         1223 g          847 t
ORIGIN      
        1 tgtcccaggc gatctttgac gccgtgcgcc ccctcgcctt cgcacttcac tgccgaccca
       61 tggacgcctt gcgccgacac tacggcctgc cgcccctcgg cccggacctg cggcgcgtgt
      121 acaccgatgc cgatctggac ttgttctgcg atctgcccga gttctacaac gccccgccac
      181 agattcccgg cggtgccttc atcgggcccg tccaatggtc accacccgtt tccctaccgg
      241 cgtggtgggc acagatcgat ggatctcggc ccatcgtcta cctcactttg ggcagctccg
      301 gcgatcctgg cttgctgcca cgcctagtag gcgccctgtc ccgcctcgac atcacgctgc
      361 tggtcgcaag cgccggcgcg cccgtcggcc cggcacgcga caacgtcctg cacgccgact
      421 atctgccggg cgattgcgtt gtcgatcgcg ccgatctggt ggtctgcaac ggcggcagcc
      481 cgacctgtgg tcaggccttt gccgccggcg tgcccgttct gggcatcccg ggcaacctcg
      541 accaatttct caacatgtat tacgtggagc ggaaagggct cggcctgacc ctgcgaagca
      601 cccagcttcc tgacgcggtg ccaaccgcgt gtcatcgcat tcttgaagat actgcctacc
      661 gcgaatcggc gcgcggagtc gctctcctca tggaacgctc gtccaccgcc gtgcgtctgc
      721 gggaagccct cgcgccactc ctcgcatgaa ttttaagcat ggcggggacg gcgcagggcg
      781 acccggacat ccgggcgcac ccgacgcacc cgtcgtcacc taggagccgg gatcacccgc
      841 gcttgctgat caacgcaccc gtttgcgggc caccaccagc atgtcgcact gactcgggaa
      901 caaacgcccg aagaaccccc agaacagcag actcgggtgc ttgatgaaat gcatcggact
      961 gaccggatcg aagccgatgt ccatgaatcc accaccgctc tgaaaatacc ggaacaggaa
     1021 agctgaattg acgaagggca cccacgtgtc cacgtaccat tccttacgga aatcaacgac
     1081 ctcccagtca tttcgcgcca catcgcgctg caactcggcg gccgcataga tatgccgatg
     1141 ataaggcgcg tgaatggcgt tctggtcgag ttcgtcgtgc agatccagac cgtccgcatt
     1201 gggtgtgccg atgaccagat aacccccagg cgccacgtat cgcttgagtt cgtcgatcac
     1261 cgcggacggg ttatccacat gctcgatcac atcctgggat gtgacgaact ggtattggcg
     1321 cgcgagcacg gatgcgtcgc cgaattcact tgagtaggga tcgtagccct cggccagaat
     1381 gccctgcgcc cgcgcgtagc ggacaaagtg tccgctaccg cagccgtgat cgagcagcgt
     1441 cgcgcccttt gtcagcccca cgccagacaa caatttgatt cgcttactgc aggtcagacg
     1501 cgtgaaaccg tcgtattgct ggcgcttgat cgggtaattg cggtaatagc gatcgtaatc
     1561 gatcgactca agaacgtgaa gtgaatggca tcggccacag cgccatacag taaacacttc
     1621 accggcaaac tcccgcacat aacacggaac tctggcggaa tctgccgata gcatgtccgc
     1681 cttccgagtg ccgcagaaca cgcactttac atttgcannn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnccc aggctggcga gcgcgacgga ggtcggctcg gggatgcgct
     1861 tgaggtcaaa gttgctggcg gcatcgcttt gcagaagcag actgttgccg ttcagaccgt
     1921 tgatggtacc gatcaccgag gaatcggctg ccagttgccc agcgaccaga cgggacggat
     1981 cgacttgacc gaacggcaga cccagattgg tctgcaggaa aatggtcatg gcgtcaccaa
     2041 cggacactcc cgtgaagtag ttcgtgtcaa tataaccaat ggtcagattg acgtcgccag
     2101 agccggaacc cgtggtggta tacagggtcg ggtaatcatt gttgccgttg gcttgatcca
     2161 gagcgacgac gttgctcttc ggatccgagg tgaacttggt gtcgccagcg ccggtcgaag
     2221 aattgtaggg gtcgatggta cccgacagga tcttgatacc gttgttgtag ccagtgccag
     2281 ccagcgtgtt ggaatcgaca gcgctgaccc acacttcaaa gtagttggtg ccacccgtga
     2341 cagactgaag gctcagggaa gcgggaccat tcagcggatc cgtcgggtcg atgctgatcg
     2401 cagagacctg ctcgcggaaa gcacccacga aggtccattc gtaggtgttg ttcagcccag
     2461 gcgaggtaac agttttgcca agattgttgc ggacggagcc caaggtgcct tggacatagg
     2521 tctgcaggag ttggccaaca taggggctat tgacggagtc acccggagta accggggtga
     2581 tcagggcgga atcaggcgac cagtcgaggc tgctggtctt aatggcagca tcggcgccgg
     2641 cgccgtcgta gtcgatggtc acaacgacgc tcgcttgggc gccagcggca gccagggcca
     2701 gagcggcggc gaggagggac ttcttgaaca taggggaatc tcctaggtag tagtgtcagt
     2761 attagagctg aagatcaact cacaccgttg tttagcaaac cctgtgccaa aaaataaaaa
     2821 ccatttaaaa acataccatt atgtagaaca cgggcatcca agcaggccac cccagcgaga
     2881 atgtgtaaaa aactccgaca tcttcaggaa gcccctcctt acaccccagc accgcacccc
     2941 atgcagcgcc caagaaaatc aagagacttt tgtcacactt aacataaagc gacaaattca
     3001 aaccggcaaa atgactgcac gtacccgcgc atcatcgacc attcgaccgg tcagtccgaa
     3061 aagcttgtcg ccccctatca cgagcggatg tttcttactg tcaggtagat cgacatcgtc
     3121 aggcttggcg cctcagagcg tccacgacgt tcgttctcga ggccttgaat gccgggaaca
     3181 cgctagcagc catcgttgtc ccaagggcga gaacgaaacc gttgatggct aaaggaagat
     3241 ccaccagaac ctcggcatca aatccttccg acatacccgg cggtggtggc aacggaatgc
     3301 ctatcttgga cacagcgatc gacagcaacc agcctccccc ggcaccaatg agcccaccga
     3361 ggaagcccag cacacagcct tccaacaaaa acagtcgcag aacgtccgag cgtctgcgcc
     3421 cgatggccat catcgttcca atctcggtcg tacgttcgat caccaccatc tgcatcgtgt
     3481 tgccaatgct gacagcgaca atcaagccga tcatgatctc aatgacgaga acttgctgct
     3541 caaagagatc aaccgtctta cggtaataat cggccaagtc aaaccacgaa tacacctgat
     3601 aatcccgacc cgccagcctc gcgcgcatga ccgccaacgt ctcatcagtc cgcggcgtct
     3661 tatctaccag gacgacccaa ctggtcgcac ctttgacctt aaccaactta cgaacgagtg
     3721 agattggcgc ccgcaacgcg cccgcatcgt attccttgtt gaacgttgaa aagatgcccc
     3781 gcacgtggac gtccgatgcg gaaagctgac ccttggcggt gttcgtcatg agtaccaagg
     3841 cgtcaccgac cttgaccccc agattctgag ccagcccttc cccaagcgcc acgccgttgg
     3901 gatcctctcg agacaagcgc tcgccggaaa gaatcgtcac atatttactg acttgaagat
     3961 catcgactgg gtcgacccct gcgcctgaaa aagtcaaggt cgcgtcgttg tagcccagca
     4021 gaccagtgaa cgccaagcgc ggggatacga cggctacctc gggcacggta cggatatccg
     4081 gatccatctt tgcgtcctct ggaagcagga agcggtaggg atctgaaata cccttatcaa
     4141 aatagccggg ccttacaatc tgtaaatggc cgaattcgga atagatgatg ccctcggcca
     4201 tgttctcgag catccaacga acaaagccac ctgtcatgag aaatgcaacc acgccaccga
     4261 cgataattgc caacgcagac agcgtcctgc gtcgatgcct ggtgacgttg cggaacgcaa
     4321 tcaccatgtc cgttgcgctg ggcaagaaat tcttgagcat gtcgttcaga tgcggctaag
     4381 caaggaacga tcatactcga tttgtacaga cgacagcgcc atgcacggcg cggtgcccag
     4441 cagagcagtg gggaggcagc ctgatcctag aggaggcccc ccgtcccaat gtgagcttca
     4501 ggccgccttc ttcatctgct tctggtacag gaactcgagg atcgccgccc ga
//
LOCUS       DAOQEW010000263         4540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8082_length_4540_cov_7.497659, whole genome shotgun sequence.
ACCESSION   DAOQEW010000263 DAOQEW010000000
VERSION     DAOQEW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8082_length_4540_cov_7.497659"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..2200)
                     /locus_tag="PLS35_18095"
     CDS             complement(353..2200)
                     /locus_tag="PLS35_18095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNI20637.1"
                     /translation="MKKARLSVAQTRVVEHGDGALLVVAGPGSGKTRVLTERVRRLLT
                     AVPGHFRILALTFTNKAANEMKERLSDLGNENQRAFIGTMHSFCLEMLSERGRPVGVE
                     GLPHVFEQHKDRKEILVEAVRQDPLLSDELASERDAKAVARRLDQWLASISWLKAHPS
                     AMDDSDDEIGKRVLEAYDAGLRACGAYDFDDLLLLTYRLLSEYPKIADFYRRLYRFVC
                     VDEAQDLNEAQYSVLAALCGDSYKSVMMVGDPKQSIYGFNTSSPDYMWRFEEEFRAER
                     IELTDNFRSSKAVVDLAKTLDPNYVVEGQLPIHGLVEVFAGADEEAEAKLVADRIQTL
                     MAEGHEDVEEPIEWESCAVLGRTRFALMAVESEFRGRGIPFYKRLTANHENESDVVED
                     FVLSLRVMANPKDRLHMSALAKRWKTTAPMPSGGAAGVDAIGLMATSAGMPRAKAVAE
                     ALAAIAAKTQRLDMLPGLDVIKAHADTLDEEGKRAVYEDIAVFEQEWDQYLRTGPSNK
                     SVGGFMSSKALGATQQASREGVALLTVHSSKGLEFDVVFVVGMAEGVFPDYRAKTRKE
                     QEEESRNAFVAVTRSKRLLYLTWPRMRQMPWGGWKNQSPSRFLSSIGRG"
     gene            complement(2210..4054)
                     /locus_tag="PLS35_18100"
     CDS             complement(2210..4054)
                     /locus_tag="PLS35_18100"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI20638.1"
                     /translation="MYKPNSGIKITDVRVSNFRTLKNVEMPLGDLTILLGANNAGKTS
                     FLDAFYAAIGAGRKILGQDDVRLDGMEALPPKERVVTVDVRIRPFDDDGKILSAFPPG
                     SFWTSLWGTGIKQEMDGNFDEFVGVRTTLAWSTSKGDYVFERRFLKEWRGFGDWLATP
                     VDETSKVNAAQIEPLVLHYIDAKRDLEDDIRKQGSFWRRLTEDLGLLVADVEAFEKVL
                     SELNHELVAKSGVLKHLKDSLADIGSIVSAEGGYIDISPVARKLRDLSKGVDVSFTAT
                     GAQSFPLARHGMGTRSLASLLVFRAFASWKAKQAEKAGDHVHSILALEEPEAHLHPQA
                     QRSFFSQVKSIPGQRVVSTHSPYFAGQAELSDLRLFIRSGGETSVSRLDLSALSSDDL
                     RKLRHGVVNTRGDLLFARAAVFFEGETEEQVLPILAEAYWGCNIHELGFSFVRVGSNS
                     NYFPFIWLVERLEIPWYVFSDGEAETITHLERALHKSGKSALAACPEVFVIPGGRNFE
                     RQLIADGYLPEIEDAFDQVEGKDSFLDDYITRMDGKAGKKGVVRDYMVADGRQKAACD
                     AMSENKTRMAEPLATTLCAITDVSRRFPATIKALFEKMSADHGLSQAS"
     gene            4333..>4540
                     /locus_tag="PLS35_18105"
     CDS             4333..>4540
                     /locus_tag="PLS35_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20639.1"
                     /translation="MTGTLGKAYIQHSRAGCFRADATPEFGRQSGIASIRLVADQIPI
                     RSASAVSAAAAPVRMRTSTIRDTEV"
BASE COUNT         1023 a         1415 c         1096 g         1006 t
ORIGIN      
        1 tcgcatcagt cgcatcaatc aggcccggcg tgcggagcaa gtgcgcacgc cgggccggtt
       61 gtttcaggtt cagttagcgg ttcggacaca cttgtattgt gcatactgtg cagacatcta
      121 aacgattcat aggaaccgcc tagtacaaac gcatccgaat catctcccgt tcaacattct
      181 gaccagatag gtctcatccc agccgacctg ccagcgcata atccttgcac agtcggcagg
      241 cgtctttggc gaaactcaca ttcagcgacc agtgctgtta gctttccacc cctcagcgca
      301 cacaccgcct cggccagcct tgaggtgtct tcttggtcct ttccatgtgc gcttaccctc
      361 tcccgataga cgataaaaac ctagaggggc tttgattttt ccaaccgccc cacggcatct
      421 gtctcattct tggccaagtc agataaagaa gacgtttaga acgagtgact gcgacgaagg
      481 cgttcctaga ctcctcctcc tgctccttcc tcgtcttcgc cctgtagtcg gggaatacac
      541 cctctgccat accaacgaca aacaccacat cgaattccag cccctttgag gaatgtaccg
      601 tcagcaacgc cacaccttct cgcgatgcct gttgggtggc tccgagcgcc ttactactca
      661 tgaagccccc cacgctcttg ttggaaggcc ccgtccggag atactggtcc cactcttgct
      721 cgaacacagc gatatcctcg tagacagccc gcttgccctc ctcgtcgagc gtatccgcat
      781 gtgccttgat gacatcaagc cctggaagca tgtccaaacg ctgtgtcttg gctgcaatgg
      841 cagccaaagc ctccgccacg gccttggccc taggcatccc tgcggaggtc gccatcaagc
      901 caatagcgtc aacccctgca gccccaccag aaggcatcgg agccgtagtc ttccatctct
      961 tggccaaagc ggacatgtgt agcctatcct tcggattggc cattacgcgc agcgaaagga
     1021 caaagtcctc caccacgtcg gactcgttct cgtggttggc cgtcaaacgc ttgtagaagg
     1081 gaatcccacg ccccctaaat tccgattcaa ctgccatcag agcaaaacgt gtgcggccga
     1141 gcactgcgca actctcccac tcgatgggct cttccacgtc ctcgtgtcct tcggccatca
     1201 gcgtttggat cctgtcggcg acgagttttg cctccgcctc ctcgtccgct ccggcaaaca
     1261 cctcgacaag tccatggata ggtaattgcc cctcgactac atagttcgga tcaagggtct
     1321 tagccaaatc caccaccgcc ttcgaagatc gaaaattatc agtcaactcg atccgctcgg
     1381 ctctgaactc ctcctcaaag cgccacatat aatcgggact ggaggtattg aagccatata
     1441 tcgattgctt ggggtccccc accatcatga cgcttttgta ggaatcgcca caaagagccg
     1501 ccaagaccga atactgtgct tcgttaagat cttgggcctc atcaacgcaa acgaaacggt
     1561 aaagccgccg ataaaagtct gcgatcttcg gatattcgct taggagccta taggtcagca
     1621 ggagcaaatc gtcgaagtcg taggctccac acgccctcag acctgcgtcg tatgcctcca
     1681 acacgcgctt tccaatctcg tcgtcagaat catccatcgc cgaggggtgg gccttgagcc
     1741 acgagatgga ggcgagccac tggtcgagcc tccttgccac ggctttggca tcgcgttcgg
     1801 acgccagttc gtcggacagc aggggatcct gtctgacagc ctcgacgagt atctccttcc
     1861 tatccttgtg ctgttcaaac acatggggca gcccctcgac accaaccggc ctgccccgct
     1921 cggaaagcat ttctaggcag aaactatgca ttgtaccgat gaaagctcgc tggttctcgt
     1981 tgccgagatc ggatagtcgc tccttcatct cgttggccgc cttgttcgtg aaggtcagcg
     2041 caagaatcct aaagtgcccc ggcactgcag tgagaagcct cctgacgcgc tcagtaagca
     2101 cgcgcgtctt gcccgagcct gggcccgcca cgacgagcag ggccccatcc ccgtgttcca
     2161 ccacacgagt ctgcgcaacc gaaagcctgg cttttttcat agtttctcct tagctggcct
     2221 gcgaaagccc gtggtcggct gacatcttct cgaacaatgc ttttatggtt gcaggaaacc
     2281 ttcgggacac atcggtgatg gcgcaaagag tcgtcgcgag cggttcagcc atacgcgtct
     2341 tgttttcaga cattgcatcg caggcagctt tctggcgacc atcagcgacc atgtaatcgc
     2401 ggaccacccc ctttttgcca gcttttccat ccattcgggt tatgtagtcg tcaagaaagg
     2461 agtcctttcc ctccacctga tcaaaagcat cctcgatttc aggcaagtaa ccgtccgcaa
     2521 tcaattgacg ctcaaaattg cgaccaccgg ggatcacgaa cacctccggg catgcagcca
     2581 gtgctgactt gccagattta tggagagctc gctccaaatg cgtaatcgtt tcagcctctc
     2641 cgtcagagaa aacgtaccac ggaatttcta gtctctcgac aagccagata aacgggaagt
     2701 agtttgagtt acttccaacc cgcacaaaac tgaagcccaa ttcgtggatg ttgcatcccc
     2761 aataagcctc ggcaagtatc gggaggacct gctcctctgt ctcaccctcg aagaagacag
     2821 cagcacgagc aaacagcaaa tctcccctcg tgttcacgac cccatgcctg agtttcctga
     2881 gatcatcgga gctcagggcc gacaggtcta gtcgcgagac tgaggtctcg cctccgctcc
     2941 gaataaacag acgcaaatca gacaactcgg cctgccctgc aaaataaggt gagtgggtac
     3001 tgacaacgcg ttgcccagga atagatttca cctgtgagaa gaacgatctc tgtgcctgcg
     3061 ggtgtagatg agcctccggc tcctcgagag cgaggataga atgtacgtga tctcctgctt
     3121 tctccgcctg tttggccttc catgaggcaa aggctctaaa cactaacaac gatgccagac
     3181 ttcgcgttcc catcccgtgc cttgccaacg ggaacgactg ggctcccgtc gccgtgaatg
     3241 agacgtccac gcccttcgac aggtctcgca atttacgggc aaccggggag atgtcgatgt
     3301 agccaccctc tgcggacaca attgaaccta tatccgcgag actatccttg agatgtttga
     3361 ggactccact cttggcgaca agttcatgat tcagttcgga aaggaccttc tcgaaggctt
     3421 caacgtcggc gaccaatagc ccaagatcct cagtcaagcg gcgccaaaac gatccctgct
     3481 tgcggatatc gtcttcaaga tcgcgcttgg cgtcgatgta gtggaggact aacggctcga
     3541 tctgggctgc attcaccttc gaagtctcgt ccaccggagt cgccagccaa tccccgaaac
     3601 cccgccactc tttgaggaaa cgtcgttcga agacgtagtc acctttcgag gtgctccatg
     3661 caagggttgt ccgaactccg acgaactcat cgaaattgcc gtccatctcc tgcttgatac
     3721 ccgttcccca gagactcgtc cagaaactac ctggcggaaa ggccgataag atttttccgt
     3781 catcgtcaaa aggcctgatt ctgacatcca cggtcacgac ccgctccttc ggaggaagag
     3841 cctccatccc atctagacgt acgtcgtcct gcccgaggat tttccggccg gcgccaatcg
     3901 ccgcatagaa cgcgtcgaga aaactagtct tgcctgcatt gttggccccg agaagtatcg
     3961 tcaggtctcc gagtggcatc tcgacgtttt tgagggttct gaaattagag acgcggacat
     4021 ccgtgatttt gataccgctg ttcggtttat acattgccgt ccctccaact aaaaaagcgt
     4081 ggcatttgta cgctcatcta ttaaccaaga tcactcgcga aacgggatca cactgatcaa
     4141 cattacatct ttgcgaagcc ctaaagcatc gtatcatcct agctatattc aatgacttga
     4201 cacgtaactt cattccgact gatccgccgg cacttgatat cgaggccatg acgagagatt
     4261 tctcctgagt agcacgatca acgcactgca ccggaccatc gccagacaaa ctggcgcatg
     4321 atccttgcgc acgtgaccgg cacgctcggc aaagcgtaca tccagcactc aagggcaggt
     4381 tgctttcgtg ccgatgcaac tcccgaattc ggccgacaat ccggcatcgc cagcatccgt
     4441 ttagtagccg atcaaatccc gatccgctcg gccagcgccg tcagtgccgc cgcggcgccg
     4501 gtgcggatgc gcacgtcgac gatccgcgac accgaagtct
//
LOCUS       DAOQEW010000264         4534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8097_length_4534_cov_8.587854, whole genome shotgun sequence.
ACCESSION   DAOQEW010000264 DAOQEW010000000
VERSION     DAOQEW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_4534_cov_8.587854"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2599)
                     /locus_tag="PLS35_18110"
     CDS             complement(158..2599)
                     /locus_tag="PLS35_18110"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNI20640.1"
                     /translation="MGVTTTAKRGDARSAPSRSSHVKREVIGVLLIAVGLLILLSLVS
                     FVPGDAKSIGASGAAGNQPKNMIGSVGALSAAACFFMVGGAAYLFPILLGLLGARCFT
                     PIPLTMRLRNAGSGLAAMVFLSALLHLEVTAVPTISSGWVNRGLAGGIIGQVLADGVR
                     SYFATTGAHIVVLTGLMVALLFTVPVSLTALLQRIPDWWIAARERMTGLMPEWPVKQE
                     EPMAPPKRARVKVARPAREMEEPELDREVQVVAEAVEQIAVPMIPPKIQPPMKVEKRA
                     TPEDEPAAAVATSPSVSDGYVLPDPQELLSDPSGPIARLSDDELKLQSEILTKALKSF
                     AIEGRVTEVRPGPVVTMYEFEPAPGTKVARIVNLADDLALALKAISLRIVAPLPGKSV
                     VGIEVPNPNREMVSMKEVVTSDAFSRSRSKLSLALGKDIFGGAVCADLKTMPHLLVAG
                     ATGAGKSVGLNTMLLSILFSARPDEVKLLLIDPKMLEFQSYDGIPHLLRPVITDPKSA
                     ARGLGWVVQEMERRYKLLAEAGVRSIDAYNRRISELHGAVSDVWQSGKAEQVELTFLS
                     EEERLSKGEDAEPAGDNGPTDSVKPSLPEPLPYIMVMIDELADLMMVAPKDVEDKIAR
                     LAQMARASGIHLVLATQRPSVDVLTGLIKANFPARIAFQVSSKTDSRTILDANGAEAL
                     LGRGDMLYLASGTGKLMRIHGSFVSDDDVRRVVEFVKKQALPSYCRELQSLKIEEAEE
                     EQAKDEVYEQAKDLVLSTGQASASLIQRRLRVGYPRAARMIEQMEAEGVVGAAGRDGR
                     REVLGRRGPVGGDEA"
     gene            complement(2698..3504)
                     /gene="rsmA"
                     /locus_tag="PLS35_18115"
     CDS             complement(2698..3504)
                     /gene="rsmA"
                     /locus_tag="PLS35_18115"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNI20641.1"
                     /translation="MAASFPPALKRLGQNFLIDPNIIRKIVSLAALRPDDTVLEIGPG
                     RGALTAGLCAEAGRVIAVEIDPQLQPQLQETLSHCRNLDLRIGDALEFPFDSLPPRTV
                     VVSNLPYYVSTPILFALLDARAHLDRLVLMLQTEVALRLAAKPNSEDYGVLSVLTQEA
                     AEVEVAFRVSANCFRPRPTVGSAVVHLKLKTQDGVDPDRYERFRRLVRAAFAHRRKTL
                     VNSLRDEGYPVERISRAMQAVGVPLQARAETLTLDDYRALATAVDDDESA"
     gene            complement(3521..3841)
                     /locus_tag="PLS35_18120"
     CDS             complement(3521..3841)
                     /locus_tag="PLS35_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20642.1"
                     /translation="MSDAGSSAATIIDQQVKELDGIEKRTIQDFNTTLGTERVGQWKI
                     RTVALLQQHAGQAEADGLSRKKPGMSFTNDLIEEFSDEVESYRSYLVTLAKRLRAAVP
                     PNAG"
     gene            complement(3860..>4534)
                     /gene="pdxA"
                     /locus_tag="PLS35_18125"
     CDS             complement(3860..>4534)
                     /gene="pdxA"
                     /locus_tag="PLS35_18125"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNI20643.1"
                     /translation="VGGIVTAPINKEAMNMAGFHYPGHTELLADLTKTKEFGMMIVGG
                     PLKIMFTTTHVAIHALSPLLTTQRIAKAIRLAHLGLTKYFGIARPKIGVAALNPHAGE
                     HGLFGNEEQTSIAPAIKLARATGINASDPLPADTLFGKAARGDYDGVVAMYHDQGLIP
                     LKLVAFGTCVNLTVGLPIIRTSVDHGTAYDIAGKGVAEHGSLLEAVKVAARLAKSWSP
                     VHSLSR"
BASE COUNT          930 a         1426 c         1287 g          891 t
ORIGIN      
        1 tccttggtct tttcatatcg cgactgaatg ttcttcacca cctcctgcac ttcgtggagc
       61 gactggaggt cgacggccgg ctcgtccggc ttcacgagtt gagccgcagc cggaatggcg
      121 ctgaccacta acaccgcaat gagtaaccct gttcgcatca tgcctcatct ccaccaaccg
      181 ggcctcgacg tccgagcacc tcgcgacgtc catcccgtcc ggccgcgccc acaacgcctt
      241 ccgcctccat ctgttcgatc atgcgcgctg cccggggata gcccacccgc aagcgccgct
      301 ggatcaacga ggccgaagcc tggcctgtcg agaggacgag gtccttcgct tgttcataca
      361 cttcgtcttt cgcctgctcc tcttccgctt cctcgatctt gagcgattgc agttcgcgac
      421 aatacgaggg caaggcctgc ttctttacaa actcgaccac tcgccgcaca tcgtcgtccg
      481 agacaaacga tccgtggata cgcatcaatt tcccggttcc cgacgcgagg tacaacatat
      541 ctccgcggcc gagcagcgct tccgcgccgt tcgcgtccag gatcgtgcgg gagtcggttt
      601 tggacgagac ttgaaacgcg atgcgggccg ggaaattggc cttgatgaga ccggtcagca
      661 catcgaccga cggccgttgt gtcgccagta ccaggtgaat gcctgaggcg cgcgccatct
      721 gagccagtct ggcaatcttg tcttccacat ctttcggggc caccatcatg agatcggcca
      781 actcgtcgat catgaccatg atatagggca agggctcagg caggctcggc ttgaccgaat
      841 cggtcggccc gttgtcgccg gctggctctg catcttcccc cttggaaagc cgttcttctt
      901 cggacagaaa cgtcaattcc acctgttcgg ctttcccgga ttgccagaca tcggacacgg
      961 ccccatggag ctctgaaatc cgccgattat aggcatcgat gctgcgcacg ccggcttcag
     1021 ccagcaactt gtaccgccgc tccatttcct gaaccaccca tcccaggcca cgcgcagcgg
     1081 acttgggatc ggtgatcacg ggacgcagca aatgcggaat cccgtcgtaa ctctggaact
     1141 ccagcatttt aggatcgatg aggagcagct tgacttcatc cggccgcgcg ctgaacagaa
     1201 tgctgagcaa catcgtattg agaccgacgc tcttgccggc accggtggcc cccgcgacca
     1261 gcagatgcgg catggtcttc agatcggcac agactgcgcc gccgaaaatg tctttgccga
     1321 gcgccagact caatttcgag cgggagcgtg aaaaagcgtc gctggtcacg acctccttca
     1381 tggacaccat ttcccggttc gggttcggca cttcaatgcc gacgacggac ttgccaggca
     1441 agggggccac gatgcgcaaa ctgatcgcct tgagcgcaag ggccaggtca tcggccaggt
     1501 tcacgatgcg cgcgaccttg gtgccgggcg ccggctcgaa ctcatacatg gtgacgacgg
     1561 gccctggacg cacttcggtg acgcgtccct cgatcgcaaa actcttcaag gccttggtca
     1621 ggatctccga ttgaagtttc aattcatcgt cgctgaggcg agcgatagga ccggacggat
     1681 cactaagtaa ttcttgcgga tccggcaaca catatccgtc ggacacggat ggactcgtcg
     1741 ccaccgccgc agccggttcg tcttcaggcg tagcacgctt ctcgactttc atcggcggct
     1801 gaattttggg aggaatcatg gggaccgcga tttgctcaac agcctcggcc accacctgca
     1861 cttcgcggtc gagttccggt tcttccatct cacgtgcggg gcgtgcgact ttcacacgag
     1921 cccgcttcgg tggcgccatc ggctcttcct gcttgaccgg ccactccggc atgagccccg
     1981 tcatccgctc acgggcggcg atccaccagt ccggaatcct ctgcagcaat gccgttagcg
     2041 acacaggcac cgtaaagagc agggccacca tcaaccccgt gagcaccaca atatgcgcgc
     2101 ccgtcgtggc gaaataactg cgcactccat cggccaacac ctggccgatg atcccgccgg
     2161 ctaagccgcg attgacccat ccgctggaaa tcgtaggcac cgctgtgact tcgaggtgca
     2221 agagcgcgct caaaaacacc atggccgcca gtccgctgcc cgcattcctg agtcgcatgg
     2281 tgagggggat gggagtgaaa cagcgggcac cgagaagccc gagcagaatc ggaaagaggt
     2341 aggcggcccc accgaccatg aagaaacaag cggccgccga cagcgctccg accgacccga
     2401 tcatgttttt gggctgattg ccggctgctc cggatgcccc aatcgatttg gcgtcgccgg
     2461 gaacaaacga caccagactg agcaggatca agagaccgac tgcgatcagg agaaccccga
     2521 tcacttcgcg cttcacatga gaggaacggg aaggggctga gcgggcgtca ccgcgctttg
     2581 ccgtggtggt tacacccatg cggcggatcg tagcacaggg aaaatacctt ttcaaacaaa
     2641 gcggcaatgt tgcgcatggc tatcacgatg ctcccatttc cgtcgagacc gtcgtgatca
     2701 agccgactca tcatcgtcga ccgccgtagc gagggcgcga tagtcatcga gggtcagcgt
     2761 ctccgcgcga gcctgcaacg gcacaccaac ggcttgcatc gcccgggaaa tccgctcgac
     2821 cggataaccc tcgtcgcgca acgaattcac caacgtcttc cgccgatgag cgaaggcggc
     2881 ccgcaccaac cgccggaacc gctcataccg gtccggatcg acgccgtcct gggtcttgag
     2941 cttgagatgt acgactgcgg atccaacagt tggacgaggg cggaaacaat tcgccgacac
     3001 gcgaaacgcc acctccacct ctgccgcctc ttgcgtcagg accgacaaga caccatagtc
     3061 ctcgctgttc ggtttcgctg ccagcctgag cgccacttcc gtttgcagca tgaggaccag
     3121 acgatcgaga tgggcacggg catcgagtaa cgcaaacagg atcggcgtgg acacataata
     3181 cggcaggttg gagaccacca ccgtccgcgg cggcaaacta tcgaagggaa attccagcgc
     3241 atcgccgata cgaaggtcaa ggttccggca atgactgagc gtctcctgta attggggctg
     3301 cagttgcgga tcgatctcga ctgcaatgac gcgtccggct tcggcgcaca acccggccgt
     3361 cagggcgcca cgcccgggtc cgatttccaa aaccgtgtcg tcgggacgaa gcgcggccag
     3421 ggatacgatt ttgcgaataa tgttcggatc gatgaggaaa ttctgaccaa gccgtttcag
     3481 ggcgggggga aaggaagcag ccatgatttc acgcggcttt ctatccggcg ttgggtggca
     3541 ccgccgctcg caggcgtttg gccaatgtca cgagatagga ccggtaagat tcgacctcgt
     3601 cgctgaactc ttcgatcaga tcgttggtaa acgacatccc gggcttcttc cgggacaggc
     3661 catccgcctc agcctgtccg gcatgctgct gaagcaacgc caccgtgcga atcttccact
     3721 ggcccacacg ttctgtgccc agggtggtgt tgaagtcctg gatggtccgc ttttcgattc
     3781 cgtccaactc cttcacctgt tggtcaatga tggttgctgc ggaagagcct gcgtctgaca
     3841 tggagatacc gcctctttcc tatcgggaca atgaatgaac aggggaccag gatttggcca
     3901 aacgagccgc caccttcact gcctcgagca gacttccatg ctcggcgacc cctttgcccg
     3961 caatatcgta cgccgttcca tggtcgaccg aggtgcggat aatcggcagg cccaccgtca
     4021 aattcacaca ggtgccgaag gccaccaact tgagggggat taaaccttga tcgtggtaca
     4081 tcgccacaac cccatcataa tcgccgcggg cggctttgcc gaacagggta tcggcaggca
     4141 gtggatcgct ggcgttgatc ccggtcgccc gggccaattt gatggctggc gcaatactgg
     4201 tctgctcctc atttccgaat aagccgtgtt cacctgcatg aggattcaac gccgcaacgc
     4261 cgatcttcgg gcgtgcgatg ccgaaatact tcgtcaggcc aagatgggcc agacgaatgg
     4321 ctttggcaat gcgctgcgtg gtcaggagcg gagacagggc atggattgcg acatgggtcg
     4381 tggtgaacat aatttttaag ggcccgccca cgatcatcat tccaaattct ttcgtcttcg
     4441 tcagatccgc cagcagttcc gtgtgccccg ggtaatgaaa gccggccatg ttcatcgctt
     4501 ctttattgat gggcgcagtg acgattccgc ccac
//
LOCUS       DAOQEW010000265         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8176_length_4509_cov_7.753031, whole genome shotgun sequence.
ACCESSION   DAOQEW010000265 DAOQEW010000000
VERSION     DAOQEW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8176_length_4509_cov_7.753031"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLS35_18130"
     CDS             <1..531
                     /locus_tag="PLS35_18130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNI20644.1"
                     /translation="EFQVDPITSSVLHADFFEVAMDKPIRVKVPIHLTGGQPIGLKEG
                     GVLHQALRELHVECLPSVLPDFIAVDASQLQINEGIHLKDIPKTEGLRFLDDPDHMVV
                     SVAAPMTDAKLESLLATGAAGAEGAKEPEVAAKGKAAAEGAAGAEAGKAGDAKAGAAA
                     PKAGAAPAKKEPEKKK"
     gene            531..1115
                     /gene="pth"
                     /locus_tag="PLS35_18135"
     CDS             531..1115
                     /gene="pth"
                     /locus_tag="PLS35_18135"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNI20645.1"
                     /translation="MALRLLVGLGNPGAEYAETRHNIGCWVIERAAARWSIRLTRKGA
                     AQRGSGRAGSKLVELAQTLDWMNLSGPPLKGLLRELGLTPESLVVVHDDLDLDPGRLR
                     IRQDGGSGGHNGLKSIIEALGTSKFVRLKVGVGRPAPRQDSADYVLEPVSSEERALYA
                     PCLDRAVDALELLLNRDVATAMNQFNVRDKLDDK"
     gene            1308..2513
                     /locus_tag="PLS35_18140"
     CDS             1308..2513
                     /locus_tag="PLS35_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20646.1"
                     /translation="MTMPVQSPLSLAAFDLSPERGFLPATDPLDQLPEEPIINQIGTE
                     LPKFLTARQFRQFIQDRSDIMGPVADDWGLDVFRSAMRTLSFAGHAYVWEDPDHPAPS
                     LPASIAVPWYRIAQQLGRPPVLSYASYALHNWRRINPHQPIELGNIVLLQNFLGGLDE
                     EWFVLIHIDIEAKAGPGLQGLIDAQNAAQADDAESVLAGLRALAVAQEAMRETLLRMP
                     ERCDPYIYYSRVRPYIHGWKNSQALPQGLMYEGVTDYDERPQQFRGETGSQSSIVPAL
                     DAGLGVLHADDPLTQYLVEMRDYMPPRHRAFVAALEARTDGQQRPLLYGYARDRKMTH
                     PQLWKAFCTCVDLLAQFRDIHVGYAERYIFRQHQSHASNPTSVGTGGTPFMPYLNKHL
                     EETKQLIQD"
     gene            2605..2817
                     /locus_tag="PLS35_18145"
     CDS             2605..2817
                     /locus_tag="PLS35_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20647.1"
                     /translation="MRKPGEPIYLRIHLIALALVLLATVALPRAAEFFLGPLSFGTRA
                     LAGIAIAVAGGIALYVLYNSSARNEP"
     gene            3020..3658
                     /locus_tag="PLS35_18150"
     CDS             3020..3658
                     /locus_tag="PLS35_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI20648.1"
                     /translation="MAWIRYVRSRLSVVSLLAVLLLAPVASEAEWYAAAQLGVNFADP
                     IRNVRGSGSLAGLEAPNFNLKTSPAFGGKLGVFPNHGILGIELDVSHSTPHIKNLDDV
                     PGIHLSVTNIGPSVILRYPGMTWQPYIGVGPALLVAHLGRSTTTESDTQVSLGANVLA
                     GIRAFVTPKVAVFTEYKYTDSTFRFGGAFGPVGGFDGTYRAHQLFMGLSYHF"
     gene            3697..3969
                     /locus_tag="PLS35_18155"
     CDS             3697..3969
                     /locus_tag="PLS35_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20649.1"
                     /translation="MIGSLLLTAGCAESWHQGQRHTVWSHQYNAGYGSAPVYGGAGYW
                     WNPFGYYGPGYGWYGGGSGGSHHSSSAPAPRPTPPDNAPPQFQKKY"
     gene            4051..4386
                     /locus_tag="PLS35_18160"
     CDS             4051..4386
                     /locus_tag="PLS35_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20650.1"
                     /translation="MAFCAVILMSACAQSVHQTQRLAQQDFMLDPGYAGAQNYSYWPG
                     PGYGYGIGYGAGYWGGPLGYYGGPYGPLGFGWYGGYGGFGAARPPSSMQGPRPSPPPH
                     APPQFRKKY"
BASE COUNT          894 a         1286 c         1346 g          983 t
ORIGIN      
        1 gagtttcaag tcgatccgat cacgagcagc gtgttgcatg cggatttctt cgaagtcgcc
       61 atggataagc cgatccgcgt gaaggtgcct atccatttga ccggcggtca gccgatcggc
      121 ctcaaagagg gcggcgtact gcatcaggcc ttgcgcgaac tgcatgtcga atgtctcccg
      181 tctgtgttgc cggacttcat cgccgtcgac gcttcgcaat tgcagatcaa tgaaggtatc
      241 catctcaagg atattcccaa gaccgaaggc ttgcgcttcc tggatgatcc cgaccacatg
      301 gtcgtgagcg tggcggcgcc gatgacagat gccaagctgg aatccctgct tgccaccggt
      361 gcagccgggg ctgaaggcgc caaggaaccg gaagtggcgg ccaaggggaa agcggctgct
      421 gaaggggcgg ctggtgcgga agccggtaag gccggtgacg ccaaggccgg ggcggctgcg
      481 cccaaggcag gggcggctcc agccaagaag gaaccagaga agaagaagta gtggcgttgc
      541 gccttctcgt tggattggga aatcccggag ccgagtatgc cgagacgcgg cacaacatcg
      601 gctgctgggt gatcgaacgg gcggcggcgc gatggtcgat acgtctcacc aggaaaggcg
      661 cggcccagcg aggctcgggt cgcgccggtt ccaaactcgt cgagctggcc caaacgctcg
      721 actggatgaa tcttagcggt ccccccctca aaggtctcct tcgtgaactg ggtctgacgc
      781 ccgagtcctt ggtcgtcgtg catgacgacc tggatctgga cccgggtcga ttgcgcatca
      841 ggcaagacgg cggcagcggt ggccacaatg gtctcaagtc gatcatcgaa gctctgggca
      901 catctaagtt cgtccgcttg aaggtcggag tggggcgccc ggctccgcgg caagactccg
      961 cagactatgt actggagcca gtctcatctg aggaacgagc cctgtacgcc ccctgtcttg
     1021 accgcgccgt cgatgcgctg gagttgctct tgaatcgcga tgtcgccacg gcgatgaatc
     1081 aattcaacgt gcgggacaag ctggacgaca aataagtatt gttcaatccc tctcacctgt
     1141 ccggactatt cgtcatctcc tccaaaacca acgcttttat tcggtgacgt gttcattcga
     1201 agaacgtggt cctacgatga cgcattcatg gcagggcttc tcaagtcggc agcagccatc
     1261 tctctccctg tggtagagtc aactattcgc gttaccttgg gagaatcatg acgatgccag
     1321 ttcaatcgcc tctctcactc gccgcgttcg acctctcccc tgagcgcggc tttcttcctg
     1381 ccacagaccc cttggaccag cttcctgaag agccaatcat caaccagatc gggacggagt
     1441 taccgaagtt cctcaccgcc cggcagttcc gtcagtttat tcaggatcgg tcagatatca
     1501 tgggccctgt ggcggacgat tgggggcttg atgtcttccg ttcggccatg cgaaccctgt
     1561 cgtttgccgg ccatgcctac gtgtgggagg acccggacca tccagctccc agtttgccgg
     1621 ccagcatcgc tgtgccgtgg tatcgcatcg ctcagcaatt aggccgtccg ccggtcctgt
     1681 cgtacgcctc ctatgcgttg cacaactggc gccggatcaa tccacaccaa ccgattgagc
     1741 tcggaaacat tgtgctcctt cagaatttcc tcggcgggct tgatgaggaa tggtttgtgt
     1801 tgatccacat cgatatcgaa gcgaaggccg gacctggatt gcaggggctg attgatgcgc
     1861 agaatgccgc gcaggcggat gacgcggaat cggtgctggc cgggttacgt gcgctcgccg
     1921 ttgcgcagga ggcgatgcgg gagacactcc ttcgtatgcc ggaacggtgc gatccctaca
     1981 tttattacag ccgcgtacga ccctatatcc atggatggaa aaatagtcag gcactgccac
     2041 aggggctgat gtacgaaggg gtcacggact atgatgaacg cccgcagcaa ttccgcggag
     2101 aaaccggctc gcaaagttcc attgtgccag cgttggatgc cgggcttggt gtgctccatg
     2161 ccgatgatcc cctgacacaa tatctggtcg agatgcggga ctatatgccg ccacgccacc
     2221 gcgcatttgt ggctgcactg gaggcgcgta cagacgggca gcagcggcca ctgctttacg
     2281 ggtatgctcg cgaccgtaag atgacgcatc cgcaattatg gaaggcattc tgcacctgcg
     2341 tggacttgct ggcccagttt cgtgacattc atgtcgggta tgccgagcgc tacatctttc
     2401 gtcaacacca gtcgcatgcg agtaatccga cctccgtggg cacaggtggg acgccattta
     2461 tgccctatct gaacaagcat cttgaggaga ccaagcaact catccaggac taaccgcgca
     2521 gggacgatgg cgccgcgcct cgtttcggac tggaatactg cggctggagt tcgtacactg
     2581 cgccctgttg atacttgttg atctatgcgt aaacccggcg aacccattta ccttcggata
     2641 cacctgatcg ctctggcctt ggtgctgctg gccacggtgg cattgcctcg cgcagcggaa
     2701 ttttttcttg gcccgctgag ctttggcacc cgcgcgttgg cggggattgc aattgctgtc
     2761 gcgggtggca ttgcgctgta cgtcctttat aattcttctg ccaggaatga gccgtaacag
     2821 ttaagaggct cttcttcgat tgcactgcag tgtcaccgaa tcgaccacga actatcctgc
     2881 attgatccgt ctcgcgcagc gacccattga gggcacgtgt aattcgacat cgcggaagtc
     2941 agaacagcaa agttgtccag tcttctacgc cggacgcctg ttatgatgtt agtaagcctc
     3001 gcccatggag cacttcacca tggcatggat tcgttatgtg cgcagcaggc tttcagtcgt
     3061 atcgttgttg gcagtactcc tccttgctcc cgtcgcatcc gaggcagaat ggtatgcggc
     3121 cgctcagctg ggggtgaatt tcgccgatcc tatccggaac gtgcgaggaa gtggatctct
     3181 cgccgggttg gaggcgccta atttcaatct gaagaccagt cccgccttcg gcggaaagct
     3241 gggcgtgttt cccaatcatg ggatcctggg aattgagtta gatgtctccc atagcactcc
     3301 gcatatcaag aatctcgacg atgtgccggg cattcacctc tctgtgacca atatcggacc
     3361 gagcgtcatt ctgcggtatc ccggcatgac gtggcagccg tatattggcg ttggcccagc
     3421 cctgttggtg gcgcatcttg gccgatctac aacgacggaa agcgacacgc aagtgtcgct
     3481 cggcgcaaat gtgctggccg gtattcgtgc cttcgtcaca ccgaaggtcg cagtgttcac
     3541 cgaatataag tacaccgatt cgacgtttcg cttcggcgga gcgttcgggc cggtaggcgg
     3601 attcgacggg acctatcgcg cccatcaact gttcatgggg ctgtcttacc acttctagcg
     3661 aggtgcggca tgatacgagc aattatctgg agctgcatga ttggtagcct cctgctgacg
     3721 gcgggctgcg cggagtcctg gcatcagggg cagcggcaca cagtatggag ccaccagtac
     3781 aatgccgggt atgggagcgc accggtctat gggggagcag gatactggtg gaatccattc
     3841 ggatattatg gccccggcta tggatggtat ggcggcggct ctggcggcag ccatcactca
     3901 tcgagtgcgc cggcacctcg gccgactccg ccggataacg cgccgcccca gttccagaaa
     3961 aagtattgat tcggagagat catcgtcgcc atgcacagac gaatggtaat tgcacatgaa
     4021 gcagtacgta cacgcctccg cgcatgcgcc gtggcattct gtgccgtgat cttgatgtcg
     4081 gcctgtgccc agtcagtgca ccagacgcaa cgactggccc agcaagactt catgctcgac
     4141 cccggctatg cgggcgccca aaattattcc tattggcctg gacccggata cgggtacggc
     4201 atcggatatg gggcggggta ttggggcggg ccgctgggct actatggtgg accgtacggt
     4261 ccattaggct ttggctggta cggtggatat ggaggattcg gtgcggcgcg tccgccatcg
     4321 agtatgcagg gaccgcgtcc ttcgccaccg ccacacgcgc ctccgcagtt cagaaagaag
     4381 tactgagtgg aggtgcagcg ctgcgattct gctctgttag tgaatcgcct gaagtgagaa
     4441 gcgccgtatc aatgtgatac gcaccgcctg aatccattct gtatccattc caatactgcc
     4501 ttgtatcca
//
LOCUS       DAOQEW010000266         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8275_length_4474_cov_9.016067, whole genome shotgun sequence.
ACCESSION   DAOQEW010000266 DAOQEW010000000
VERSION     DAOQEW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8275_length_4474_cov_9.016067"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLS35_18165"
     CDS             <1..816
                     /locus_tag="PLS35_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNI20651.1"
                     /translation="ACANCHMPGLAFTDGQAVSMGINRLQGGRSAPTAINRVYSKAQF
                     WDGRAQTLEEQSTGPFINPVEHGFLDYDEMTTKMKKLSGYRQLFQEVFHDDITEKNIG
                     KAIASFQRTLISGNSPADRFDSGGDEQALSASAKRGLELFRGKARCTRCHSGFNFTDE
                     KFHNLGIGWDTNTVDLGRYMVTKNPDDIGAFKTPTLREIAHTAPYMHDGRFATLEEVV
                     RFYNQGGIQNPHQDNTIIPLELTETEEQEVVAFLRSLNGEGWQQVRAPIEFPK"
     gene            956..2581
                     /locus_tag="PLS35_18170"
     CDS             956..2581
                     /locus_tag="PLS35_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20652.1"
                     /translation="MMIAVSRWIDRNILELARDFQLSYLPPIMVYMAAGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWALKMPFGHLVDLLWRWKSLLVYFGAGVIAASL
                     LIMVGLIGHREAMVAVMPAEVWYVLSALLAPIGYVIQDTVADAMTVEAVPRVDEAGLP
                     IDEAKLKLMHTTMQTLGRVAIISGGILVALINLYLFTGVENLPKDKVAEIYQQVYLLA
                     LTIPAVSVLGVITASVLRLRAKRRFLSQGMTSAQVATLLDRPEEPTKPNWWILGGSLL
                     FVLFTLTVGMADFPFNEEIVFVGSMAIVSVLMVRLTRELAPDARHVLVGTALVVFVFR
                     AVPGPGEGATWWMIDKLGFDQHFLSVLSLIGSALTLAGMFLFRRFMAERSIAYVVGFL
                     TVAAFVLGLPIVSMYYGLHEWTAALTGGVVDARFIALVNTALESPLGQISMIPMLAWI
                     ANSAPANLKATYFAVMASFTNLALSLSQLGTKYLNELFVVTREVRDPVTNAIQTPDDY
                     SQLGILLIVQTVLGLALPFAAILFSRFSRFRSA"
     gene            2602..3552
                     /locus_tag="PLS35_18175"
     CDS             2602..3552
                     /locus_tag="PLS35_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNI20653.1"
                     /translation="MHTFDFLAEHGALVLFWVIFVEQIGFPLPALPLLVAAGALVATE
                     NMSLAAALLVPVAASLPPDLAWYYLGRQKGGKVLGFLCRLSLEPDSCVRDTENVFHRN
                     GPRALLLAKFVPGFSTVAPPLAGIVGMPATTFILYDLGGTLIWAAVSAGVGALFSNQL
                     EQLIGLFDQAGGLMLVILLLGLAGFVGYKFYHRQKFLRHLRMAKISVDELKRRLDAGE
                     SISVVDVRHPLALDLDPESIPGAINFTLEDIEHRHHEIPRDRDIVLYCSCPNEVSSAR
                     TAFLLKKKGIHRVRPLEGGLDAWRERQFPVERRVNIELTQ"
     gene            3584..4204
                     /locus_tag="PLS35_18180"
     CDS             3584..4204
                     /locus_tag="PLS35_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20654.1"
                     /translation="MPMTGLALPVIAGIVLLSGGAETSAGRDWKTGSDTYAIREVKSP
                     SRQKAAGVTYKVVGVFQRDKQFIGGSAVADVTEQVEFEFTWPLVEGVQPKVLPNIPTK
                     VDNLRDGSAVCPAPVLKSTYEHFDLKNIAVIGTMFGLVGERRTPDMEEYVGGGGCKGT
                     HPVQGEVREEQLLVTVPPEILAGGVPKGQVLKQQDQGWTWTFTPLP"
BASE COUNT          844 a         1228 c         1387 g         1015 t
ORIGIN      
        1 gcctgtgcga attgccacat gccgggcttg gcattcaccg acggacaagc cgtgtccatg
       61 gggatcaatc ggctgcaagg tggtcgaagt gctcccacgg ccatcaatcg cgtgtacagc
      121 aaggcgcagt tttgggacgg gcgcgctcag accctcgagg aacaatcgac cgggccgttc
      181 atcaatcctg tcgaacacgg ctttctggat tatgatgaaa tgacgacgaa gatgaaaaag
      241 ctgagcggct accgccagct ctttcaagag gtgtttcatg acgacattac ggagaagaat
      301 attggcaaag cgattgccag ttttcagcgc acgctgattt cgggcaatag tcctgcggat
      361 cgattcgatt ccggaggaga tgagcaggcc ttgagcgctt ccgccaaacg aggcctcgaa
      421 ttgttccgcg gcaaggcacg gtgcacccgt tgccactccg gcttcaactt caccgatgag
      481 aaatttcaca atctgggcat cggctgggat accaacaccg tggatctcgg ccggtatatg
      541 gtgactaaaa acccagacga tattggcgca ttcaaaacgc cgactctgcg tgaaattgcg
      601 cacaccgcgc catacatgca tgacgggcgg ttcgcgacgc tggaggaggt ggtccgtttt
      661 tacaaccagg gtggcatcca gaatccccac caggacaata cgatcattcc gctcgaactg
      721 accgaaacgg aagagcagga ggtagtggct ttcttgcgat cgctgaacgg cgagggctgg
      781 cagcaggtga gagcgcccat cgagtttccc aaataagcgt ctgtcatggc gagggggtcc
      841 cggttcgaag accggggccc tctcgtgtta ctgccgccct gcttgcaaat gcctgtccgg
      901 tacgggtaac agagaggagg gcaatggggc ctgcaccggt tctggttacc gccatatgat
      961 gatcgccgtt tcccgctgga tcgaccgtaa tattctggaa ctcgcccgcg acttccaact
     1021 atcctatttg cctcccatca tggtctacat ggcggccggc atttccgggc tcaccggtat
     1081 cgtcggcacg ttcttcgtga aagattatct cggcctttcc gccgcctttc tcgccgccct
     1141 gggattctgg gccggtatcc cctgggcgct gaagatgcca ttcgggcatc tggtcgatct
     1201 cctgtggcga tggaagagcc tcctcgtcta tttcggtgcg ggcgtcattg cggccagtct
     1261 gctgatcatg gtcggcttga tcggtcatcg cgaggcaatg gtggcggtga tgccggctga
     1321 agtctggtac gtgctgagcg cgctgctcgc ccctatcggc tatgtgattc aagacacggt
     1381 tgctgatgcc atgacggtgg aggcggtccc gagagttgac gaggcagggc ttcccattga
     1441 tgaagccaag ctgaagctca tgcacacgac gatgcagacg ctcggccgtg tggccatcat
     1501 cagcggcggg attctggtcg ccctcatcaa tctgtatctg ttcacgggtg tcgagaatct
     1561 gccaaaagac aaagtcgctg agatttatca gcaggtctat ctcctggcgc tgactatccc
     1621 ggccgtgtcg gtactcggag tcatcaccgc gtcggtgctt cggctgcgag ccaagcgccg
     1681 gtttctcagc cagggcatga catctgctca agttgccacg ctgctggaca ggcctgagga
     1741 accaaccaag cccaattggt ggattctcgg aggcagcctg ctgttcgtct tgtttaccct
     1801 gacggtgggc atggcggatt ttccgttcaa cgaagaaatc gtcttcgtcg gatcgatggc
     1861 catcgtctcg gtcttgatgg tgcggctgac tcgcgaactg gctcccgacg ccaggcatgt
     1921 tctcgtgggc acggctctgg tggtgtttgt ctttcgcgcg gtgccgggac ccggcgaagg
     1981 cgcgacctgg tggatgatcg acaagctggg cttcgatcaa cattttcttt cggtgttatc
     2041 gctcatcggc agtgccttga ccctggccgg catgtttctc tttcggcgat ttatggccga
     2101 acggtccatt gcctatgtcg tcggctttct taccgtggcg gcctttgtcc tcgggctccc
     2161 catcgtcagc atgtattacg ggctgcacga atggaccgcg gcgctgaccg gcggtgtagt
     2221 ggatgcgcgg tttatcgcct tggtgaatac ggcgctcgaa tcgcccctcg gccagatctc
     2281 catgattccc atgttggcct ggattgcgaa ctccgcgccg gcgaatttga aggccacgta
     2341 tttcgccgtc atggcctcct tcaccaatct ggcgttgtcg ctcagccagc tcgggacgaa
     2401 atatctcaac gagctttttg tggtgactcg ggaggtgcgc gatccggtca ccaacgccat
     2461 tcagacgcct gatgattaca gtcagctggg aatattgctg attgtgcaaa cggtgttggg
     2521 cctcgcgctc ccctttgcgg ctattctctt ttcgcggttc tcgcgtttcc ggagtgccta
     2581 agcgggagga cgtcatccgc tatgcatacg ttcgactttc tcgccgagca tggggccttg
     2641 gtgctcttct gggtgatctt cgtcgagcag atcgggtttc cgctccctgc gcttccactc
     2701 ctcgtggccg caggcgcgct ggttgcgaca gaaaacatga gtctggcggc ggcgttgctg
     2761 gtgccggttg ctgcttctct tccgccggat cttgcctggt attacctggg tcgtcagaag
     2821 ggcgggaagg tgctgggctt tctctgccgg ctgtcgctgg aacccgattc ctgtgtgcgg
     2881 gatacggaaa atgtgtttca ccggaacggc cctcgcgcat tgctgttggc caaatttgtt
     2941 cccggcttca gcacggtggc gcctcccctc gccggcattg tcggcatgcc cgccacgacg
     3001 tttatcttgt acgatctcgg cggcacgctg atttgggcgg cggtcagcgc cggagtcggc
     3061 gcgttgttca gcaatcagct ggaacagctc atcggactat ttgaccaggc gggaggactg
     3121 atgctcgtga tccttctgct gggtctggcg ggattcgtcg gctataagtt ttatcaccgg
     3181 cagaaattcc tgcggcatct gcgcatggcg aaaatttccg tcgatgagct caagcggcgt
     3241 ctggatgcgg gggagtctat cagcgtggtg gatgtccggc atccgctggc cctcgatctg
     3301 gatcccgaaa gcattcccgg cgctatcaac tttacgttgg aagacatcga gcatcggcat
     3361 cacgagatcc cgcgcgatcg cgacatcgtg ctctactgca gctgcccgaa tgaagtctcc
     3421 agcgccagaa cggccttttt gctcaagaag aaaggcattc accgggtgag gcctctggaa
     3481 ggcgggctgg atgcctggcg ggagcgtcag tttcccgtcg agcggcgggt caatatcgag
     3541 ctgactcagt gagtctgtgg aatcttcagg cgaggaaagg acgatgccta tgacgggtct
     3601 ggcgcttcca gtgatcgcag ggatcgtcct gctctccggc ggggcggaga cgagcgcagg
     3661 gagagactgg aagacgggtt ccgataccta cgcgattcgg gaggtgaaga gtccctcgcg
     3721 ccagaaagcc gcaggcgtga cgtacaaggt cgtgggagtc ttccagcgag acaaacagtt
     3781 tatcggggga tctgccgtgg ccgatgtgac cgagcaggtg gaatttgaat ttacctggcc
     3841 gctggtggaa ggggtgcaac ccaaggtgct cccgaatatt ccgacgaaag tggataacct
     3901 ccgtgatggt tcggcagtgt gcccggcacc ggtgctcaaa agcacgtacg aacacttcga
     3961 tctcaaaaac atcgcggtga tcgggaccat gttcgggctg gtcggagagc ggagaacgcc
     4021 ggacatggag gaatatgtcg gtggaggcgg gtgcaaaggc acccatcccg tgcaagggga
     4081 ggtccgcgag gaacagttgc ttgtgaccgt accgccggaa atattggccg ggggggtgcc
     4141 gaaggggcag gtgttgaagc agcaggatca gggttggacc tggaccttca cgccgctacc
     4201 gtaatgtgcc gatcagtgag tgatcgcaca cctctgcttc ttctctggct tccccttgat
     4261 ccatgcgata agatgggtat ctcaaaagag taaatggctg gtcaaagatc ttctggcaag
     4321 cccgtagcga tgcgaaggtt cgaggcgtgc atgtcccgta cgtcgagaac ctgagcgagt
     4381 gagaacgccg ccagaaatct ttttcaacat tcgactgaaa gggggcgacc ggtttcgacg
     4441 gggatattga cgccatcgtc gcatgtcgag atct
//
LOCUS       DAOQEW010000267         4472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8284_length_4472_cov_8.625085, whole genome shotgun sequence.
ACCESSION   DAOQEW010000267 DAOQEW010000000
VERSION     DAOQEW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8284_length_4472_cov_8.625085"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..784)
                     /locus_tag="PLS35_18185"
     CDS             complement(236..784)
                     /locus_tag="PLS35_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20655.1"
                     /translation="MKYIPLIACVTFASAGVTYAANEATAGPFSGEVTVFPGNTAPLA
                     QTPSPDNTALSLLFQGLQVVRQGDGSRSDANHLALRIPLTISAPSRIKAELRGGIAAD
                     SGVECRLSLDGPDGRAVFMSRQAPQAHLKTVIPVSVSDNELKLLIGVRCTGRIGTHPV
                     FVASVDSLDLSLEAPTKPLRHR"
     gene            complement(808..2043)
                     /locus_tag="PLS35_18190"
     CDS             complement(808..2043)
                     /locus_tag="PLS35_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNI20656.1"
                     /translation="MSISSQSHKIRINRVPASAEFLPRKADREGRLGFGWTGKKPYAP
                     FYPGAYAPSGTHHGPPYGTDGGTLTSSDEGGNNGPYGSTNYGPYGSFTKVVGFGYQPD
                     LPDLRDFLIDRQCQLDPRLKPVLEDVVKLIQAGNVGGETNGKKSRSAAKTAPPSFLLN
                     QAGKLPERCDLRATGHLSPVEDQGPIGACTAHAVVGLIEYLMRAGGGNGQDMSRLFLY
                     KLSRRLLGWQGDTGAYIRTAIKSVALFGLPPENDWPYDVRRFDEEPLAYHFAYAQNFK
                     ATTYARLDGHGSGDATLDAVKRCLLDGFPVAFGFPVYSCIDAVGIGGRFVIPFPAALD
                     SLRGGHAVLAVGYDDSIETPLGAGAKGRGALLFRNSWGADWGVAGYAYLPYEYVQKLL
                     ALDFWTVFNKDWIKLEVFE"
     assembly_gap    2227..2326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2327..4177)
                     /locus_tag="PLS35_18195"
     CDS             complement(<2327..4177)
                     /locus_tag="PLS35_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20657.1"
                     /translation="MDALTARFRLPALAGAMLLLGGCAPNGLRHFDGDPWVRRNAEVL
                     DRMGALLNEFGSVGMSAPLIMAPGEKFNFEIKEGPQDYFDQAKKEIQGRAAAAEQEVS
                     LLGLSVSASVDPTQMAQYAEQLKNYHDQRLAYLRQLDERDRALRAASEAKKAAAQLTL
                     NGKLAAAKTPEDQAAAYSEYATAIASNAPDAPKAPDFPSAVPHPTGGSTPDAPEGATK
                     ARGVLPDLSKLTAPGSLLGTSPAVTSTNRSALITAAGDTAVEGILNALHSDGSPPPAA
                     LLGKKKLFAAAMVSVTPGWRTSSDFAARLTVRPTLEFGSARLEVVEYVFKELKSRFQN
                     EIDCGRLNAAGAGRSGFVIVNPDPNDSKKTLLPLPSPAAITAVSPMTDTQHLQLSSSL
                     RQRKERALQIALALQSAGLDAGAKLFYEHLSNLEKDVMTDNREVPITAFATGGQFGYE
                     IGPELWSQFDPSAAKTRAGYRLVRQSFPTLILLGFDETQLRPRLWCPSPESAPQVVEP
                     YLKLEQTRRWVPLNERAARYALSDAEVVALDDDATRAYLSPPVCSDGMCVADNVRKQI
                     ASRGNALAEELMGNFAKQVLPVCMVVPELPRCVKAQQDEERKQKDAAEKKQ"
BASE COUNT          808 a         1332 c         1351 g          881 t
ORIGIN      
        1 cgccacggcc cgcgccgaca aggcccgcca ggatcgggaa cactacgccg aggaaagccg
       61 caaacacgcc gagaagatgg cggaaaaggc cgcccggaaa caggcggaag ccgcctccgg
      121 caaaccggca cccaagccgc cggggttttg agggcgttac gggcgcactc ggccgccgcc
      181 tggcggaagc cgctgccgac tcatgaatgc cgtgctgcgt cggtcggttc cgtactcaac
      241 ggtgcctcag gggtttggtc ggagcttcca ggctcaaatc gagggaatcc acagaggcca
      301 cgaagacagg atgcgtgcca atccttcccg tacagcgcac accgatcagg agtttcaact
      361 cgttatcgct caccgagacc ggaatgacgg tctttagatg tgcttgcggc gcttgacgag
      421 acatgaagac cgcccgtccg tccggcccgt ccagactgag ccggcattca acccccgaat
      481 cggcggcgat gcccccccgc agctcggcct taatccggct gggcgcactg atcgtgagag
      541 gaatccgcaa cgccagatgg tttgcgtcgc tgcgcgatcc gtccccttgc cgcacgacct
      601 gaagcccctg gaaaagcaag gagagcgccg tattatccgg cgatggagtt tgggcgagcg
      661 gtgcagtgtt gccggggaat accgtcacct caccggaaaa cgggcctgct gttgcctcgt
      721 tggcagcgta ggtgacgcca gcagaggcga acgtgacaca ggctatcagg gggatgtatt
      781 tcatggtcgg atctccggaa catcgggtca ttcgaagact tcgagtttga tccagtcctt
      841 gttgaaaact gtccaaaaat ccaaggcaag cagtttctgg acgtattcgt aggggagata
      901 ggcgtaaccg gccacccccc agtcagcacc ccatgagtta cggaacagta acgcaccacg
      961 gcccttcgct ccggccccca gaggggtttc gatactgtcg tcgtagccca ccgcaagcac
     1021 cgcatggcca ccgcgcagac tgtccagggc ggcgggaaaa ggtatgacga agcgcccacc
     1081 gattccaacc gcgtcgatgc agctatacac cggaaaaccg aacgccaccg gaaatccgtc
     1141 gagcaggcaa cgcttcaccg catccagcgt ggcatcaccg ctaccatggc cgtccagtcg
     1201 ggcgtaggtc gtagccttga agttctgggc gtaggcaaag tgatacgcca gcggctcttc
     1261 gtcaaatctg cggacgtcat agggccaatc gttctccggt ggtaggccga acagcgcaac
     1321 gcttttgatc gccgtgcgga tgtaggcccc ggtgtcgccc tgccagccca acaggcggcg
     1381 gctcaacttg tacaggaaca gccgtgacat gtcctgtccg ttgccgcccc cggcacgcat
     1441 cagatattcg atcagtccga cgacggcatg ggcggtgcag gcgccaatgg gcccctggtc
     1501 ctccaccggg gacaggtgcc ctgtcgctct cagatcgcac cgctccggaa gcttgccggc
     1561 ctgattcagc agaaacgacg gtggagccgt cttggccgca gagcgggatt tcttgccgtt
     1621 cgtctcgccg ccgacgtttc ccgcctgaat gagcttcacg acgtcctcca gcacaggctt
     1681 gagccgagga tccagctgac actgccggtc gatgaggaaa tcccgcagat ccggcagatc
     1741 gggctggtag ccgaaaccga cgaccttggt gaaactgccg tacggaccgt agttggtact
     1801 cccgtagggg ccgttattgc caccctcgtc agacgaggtg agggtaccgc catcggttcc
     1861 atacggtggg ccgtgatggg tacccgaagg cgcataggct ccgggataaa aaggcgcgta
     1921 aggcttcttg ccggtccagc cgaaacccag ccgaccttcg cggtctgcct ttcggggcag
     1981 gaactcggcg ctggccggca cgcggttgat gcggatcttg tgggattggg acgatatgct
     2041 cacgtcagtc tcctcgattc agagggtcag ggtgccgggc tttatttcgc cgccgccgga
     2101 acagcggcac cgacttggac cttatccttc ttgtcggctt ccgccttttg cttttgcaca
     2161 gcctcttcct tctgtttctt ttccgccgcg tccttttgtt ttcgttcttc gtcctgctgc
     2221 gccttcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgt ttcttttccg
     2341 ccgcgtcctt ttgttttcgt tcttcgtcct gctgcgcctt cacgcagcgg ggtagctcgg
     2401 ggacgaccat gcacacggga agaacctgct tggcaaaatt gcccatgagc tcctcggcaa
     2461 gggcattccc acgggaagcg atctgcttgc ggacgttgtc ggccacacac atgccatcgg
     2521 agcatacagg gggcgacaga taggcacggg tggcgtcgtc atccagtgcg acgacttccg
     2581 catcgctgag cgcatagcgg gccgcgcgct cgttcagtgg cacccagcgg cgggtttgct
     2641 ccagcttgag gtacggctct accacttggg gggcgctttc gggcgacggg caccacagtc
     2701 gtggacgcag ttgtgtctca tcgaaaccca gcaggatcag cgtggggaaa ctctgtcgca
     2761 ccaaccggta gccggcgcga gttttggcgg cggacggatc aaactggctc caaagttcag
     2821 gaccgatctc gtagccaaat tgaccgccag tcgcgaaggc tgtgattggt acttcccgat
     2881 tgtcggtcat cacatccttt tccagattgc tcagatgctc atagaagagc ttggccccgg
     2941 catccagtcc cgccgactgc agggccaggg cgatctgcaa cgcacgctcc ttgcgctgac
     3001 gcaggctgct ggaaagttgt agatgttggg tgtctgtcat tggggacact gccgtaatgg
     3061 cggcaggaga cggtagcgga agtaatgtct tcttagagtc gtttggatcg ggattgacaa
     3121 tgacgaaccc tgatcgcccg gcacctgcag cgttcagccg cccgcaatcg atctcgtttt
     3181 ggaagcggga tttaagttcc ttaaagacat actcgaccac ctccagtcgt gcagatccga
     3241 actctaaagt cggtcgaacg gtgagtcgcg cagcaaagtc cgacgaggtg cgccagcccg
     3301 gcgtcactga caccatcgcg gctgcaaaca gcttcttctt gccgagcagg gctgccggcg
     3361 gtggacttcc gtcgctatgc agtgcgttca agatgccctc cacagcggta tcccctgccg
     3421 ccgtgatgag ggccgaacgg ttggtagacg tcacagcggg cgacgtcccg agcagactgc
     3481 ccggcgcggt aagcttgctg aggtcgggca ggacaccgcg ggccttggtc gcaccctccg
     3541 gcgcatccgg tgtgctaccg cctgtggggt gggggacggc gctgggaaag tccggcgcct
     3601 tcggagcatc cggtgcgttg gaggcaatcg ccgttgcgta ttcggaatac gcggccgcct
     3661 gatcctccgg tgtcttggcg gcagcgagtt tgccgttcag ggtcagctgc gcggcggcct
     3721 ttttggcctc ggatgctgcg cgcagggccc ggtcgcgttc gtcgagctgg cgtagatagg
     3781 cgaggcgctg atcatggtag ttcttcagtt gctccgcata ctgggccatc tgggtgggat
     3841 cgacggaagc cgaaacgctc agaccaagca gtgagacctc ctgttcggcc gcggcggcac
     3901 gcccctggat ttccttcttg gcttggtcga agtaatcctg agggccctcc ttgatctcga
     3961 agttgaattt ttcgcccggt gccatgatca gcggtgccga catgccgacc gaaccgaatt
     4021 cattgagcag ggcgcccatc cggtcaagca cttcggcatt gcgccggacc cagggatcgc
     4081 cgtcgaaatg cctcaagccg ttgggcgcgc agccccccag cagcagcatg gcgcccgcaa
     4141 gggctggcag gcggaatcgg gcagtaagag cgtccatgag ttgtccccgt gttgttgttg
     4201 gatgttgccg gggctcatgt gctttttgga gcccgtgaaa ttcatcgtag gtaagatttc
     4261 acggatttcc atactcaatt tgagtgattt ttctcccttt ttggatggat tgatgggcaa
     4321 taggcttggc cgcgtgattc attctttccc catctcatcc cggacaagga tatgccggga
     4381 aggaggcagt gtccgatggg aatccgcacg gaattcggcc cgcctacagc gtcggcccgt
     4441 ccttcaccca gctcagcgca tcttccagcc gg
//
LOCUS       DAOQEW010000268         4452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8355_length_4452_cov_8.641346, whole genome shotgun sequence.
ACCESSION   DAOQEW010000268 DAOQEW010000000
VERSION     DAOQEW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8355_length_4452_cov_8.641346"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..569
                     /locus_tag="PLS35_18200"
     CDS             315..569
                     /locus_tag="PLS35_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20658.1"
                     /translation="MIMQVISVVGALMVLIAYGLIQAGTWRELDAGYLALNIIGSLLL
                     GIVAIEDRRVGFVLLEFAWAGIALVGVARSLRARRAARGL"
     gene            624..1106
                     /locus_tag="PLS35_18205"
     CDS             624..1106
                     /locus_tag="PLS35_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20659.1"
                     /translation="MAGLLSADDVFEQAKNAAVGATNADEKALQIDYEALQLKFRAAL
                     GDRKVARCHINKFLPEGYEDQGRFNLILLTAGNVIFDIVIGDSYFRYDVVSVSQLDKV
                     QVIDAMWDNREKRREEPFLSLRLMHAEETHLLLALDDEERKSLLGFASAVSAVRNPEK
                     "
     gene            complement(1288..>1723)
                     /locus_tag="PLS35_18210"
     CDS             complement(1288..>1723)
                     /locus_tag="PLS35_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNI20660.1"
                     /translation="TPVGSHARELLRIEAGLAKAGPDLNEEIVPPEANLEGKAFSLSK
                     GCYPGQEVVARMDTYGNVRRHLVGLLIQDQTVPAHGSKLFSGDREVGWISSAVLSPQR
                     HAVLAFGFPLRDFSAPNTELTVEVAGTRHQATVHTLPFYQRP"
     assembly_gap    1724..1823
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1824..2236)
                     /locus_tag="PLS35_18215"
     CDS             complement(<1824..2236)
                     /locus_tag="PLS35_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HNI20661.1"
                     /translation="MKQSRLHDQHQQLGAAFEDVTGWSVPAHYGDAAAEHAAVRNAVG
                     VSDLSHRGKMRVTGDDRVRWLQSIISNDILPLQPGQGRYSSFLTHKGKMLGYFRVYVQ
                     SDAVWIEDVGEVGEATFQALRKFLLYGTKAKMENCA"
     gene            complement(2271..2657)
                     /locus_tag="PLS35_18220"
     CDS             complement(2271..2657)
                     /locus_tag="PLS35_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNI20662.1"
                     /translation="MNTTIVVAVTDIFFYTKVRDALRPQGYTLERIRNQDEVAEKMTS
                     VQPAALILNMNDLAVDAFKALETIQSNPALRSLPILAFANHEEVDTWRRAKELGVTKV
                     VSRNEFSARTKDLVDELIHPQPASQS"
     gene            complement(2673..3188)
                     /locus_tag="PLS35_18225"
     CDS             complement(2673..3188)
                     /locus_tag="PLS35_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20663.1"
                     /translation="MDVQDFLIQGDGSEEDKREAWQLFQQAYELQMKGQLEEAVSLYK
                     LSLATHQTAEAYTFLGWTYSFMGKLDEAIEECHKAIACDPHFGNPYNDIGAYLIEKGD
                     LDEAIPWFQKAMQATRYESPAFPHLNLGRVYERQGKWSEAIDCYKQALALNPDYALAK
                     KALGRLISSLN"
     gene            complement(3327..4127)
                     /locus_tag="PLS35_18230"
     CDS             complement(3327..4127)
                     /locus_tag="PLS35_18230"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HNI20664.1"
                     /translation="MGKPKPAPLRERDITRQIAREYYNEFDQLIESDVIIVGAGPSGL
                     ICAHDLARMGVKTLVVEQSLALGGGFWSGGYLMNKATICAPAHKILKEVGVPCKQIKE
                     CPGMYMVDPPHATGALIAAAYDAGAKIMNLTRVVDLILRREGVLEGVVVNSTTAEMAG
                     HDIIHVDPIALESKIVVDATGHDAVVVNLLHKRGLYQQVPGNGAMWVSRSEEEVMDRT
                     GEVSPNCFVIGLAVAAVFGTPRMGPAFGSMLLSGRYGAELIREKLKKR"
     gene            complement(4267..>4452)
                     /locus_tag="PLS35_18235"
     CDS             complement(4267..>4452)
                     /locus_tag="PLS35_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20665.1"
                     /translation="DNAAKVAHFCSMCGPHFCSMKITQDVRDYAAQKQLEEQQAIQIG
                     MKEKSEEFKKAGSEIYL"
BASE COUNT          851 a         1248 c         1256 g          997 t
ORIGIN      
        1 tccttgcaga tagaacgggt gcctgccggc gcatacactc tcgcctgaca accatcgatt
       61 aagcaactct gtccgctcat ctgtgcctcc caatcattct cgggaagtat gccggagatg
      121 cccccgcaaa tgcaatgcag cgtccggcgt tcgagaaggc tcttgccagg cggcgggcga
      181 gatggcgcaa aggctcgatg ccgtacccga tgccttgccc catcagaggg aatttcgctt
      241 gcaatcctgc cgagtcgcca ggtaccgtac cccgttctcc agtgagatgc gggctcgagt
      301 gcgcgagtat acccatgatc atgcaggtca tatcggtggt gggagccctg atggtgctca
      361 tcgcctatgg cctcattcaa gccggtacct ggcgcgaatt ggacgcaggc tatttggcct
      421 tgaacattat cggctcactg ctcctgggga tcgtagcaat tgaagatcga cgagtggggt
      481 tcgtcctgct ggaattcgcg tgggcgggaa ttgcgctggt cggagtggcc cggtcgctac
      541 gagccaggcg cgccgcacgc ggcctctgat cgtagatgac atcatacaca tcagacagtc
      601 ttgtaacgaa ggagtaaggc gttatggcag ggttgttgtc ggcggatgac gtatttgaac
      661 aggcgaagaa tgcggcggta ggagcgacca atgcggacga aaaggcgctc cagatcgatt
      721 atgaagcctt gcagctcaag ttccgcgccg cccttggtga tcgcaaagtg gcgcgatgcc
      781 atatcaacaa gtttttgccg gaaggttatg aagatcaggg ccggttcaat ctcatcctgt
      841 tgacggcggg caatgtgatt tttgacatcg tgatcggcga ttcctatttc cgctacgatg
      901 tcgtgtccgt cagccagttg gacaaggtgc aggtcatcga cgccatgtgg gacaaccggg
      961 agaagcgccg tgaggaaccc ttcttgagcc tccggctgat gcatgccgaa gaaacgcatc
     1021 tgttgttggc gctggacgat gaagagcgga agagcctgct tggattcgcg tcagccgtgt
     1081 cggccgtgcg caatcccgag aagtaaatca ccggggccat ctcgcaaaac ggggaccagc
     1141 ggtcttagtg tatgagagtc ctgcaggatg gtcaaaatgg ccgtcctgca aggccgcagc
     1201 aagtgaagtg ccgaggcgtc ccctcgggga cgttgaggct ctgagcgata cgggaacgcc
     1261 gcgggcgcgt gttttcaaca tcctgcttca gggccgctgg taaaacggaa gggtatggac
     1321 ggtggcctga tggcgcgtcc ctgccacttc caccgtcagc tcggtgttgg gcgctgagaa
     1381 gtcgcgtagc ggaaagccaa atgccaggac ggcatggcgt tgtggcgaca agacggcgct
     1441 gctgatccat cctacttccc gatctccgct gaatagcttt gatccatgcg ccggtaccgt
     1501 ctgatcctgg atcagtaagc cgactagatg ccgccgaaca ttgccatatg tgtccatgcg
     1561 cgccacgact tcctgccccg ggtagcaacc ttttgagagg ctgaaggcct ttccttccaa
     1621 attggcttcc ggcgggacaa tttcttcatt gagatcaggc ccggccttgg ccagacccgc
     1681 ttcgatccgc aacagttcgc gtgcgtggga gccgacgggt gtgnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnngcgcaat tctccatctt ggctttggtg ccgtagagga
     1861 gaaacttgcg taacgcctgg aaggtggctt caccgacttc cccgacatct tcgatccaca
     1921 cggcgtcgct ctggacgtac acccggaaat aaccgagcat tttcccctta tgggtgagaa
     1981 agctggagta gcggccctgt cccggttgaa gcggaaggat gtcgttgctg atgatgcttt
     2041 ggagccacct gacgcggtca tcgccggtca ctcgcatctt cccgcggtgc gagaggtctg
     2101 aaaccccgac agcattgcgt acggctgcat gttccgctgc ggcgtcgcca taatgggccg
     2161 gcacggacca tcctgtgacg tcttcaaatg cggcgcctag ctgctggtgc tgatcatgga
     2221 ggcgagactg tttcataagg agaccgataa tcctttatcc ggcacagccc tcaggattgg
     2281 ctggcgggct gaggatgaat aagttcgtca acgagatctt tggtgcgcgc agaaaattcg
     2341 ttccgggaca cgactttggt cacgccgagt tccttcgcgc gtcgccacgt gtcgacctct
     2401 tcatgattgg cgaaagccag aatgggcagt gagcgcaggg caggattgct ctgaatggtt
     2461 tccaacgcct tgaaggcatc gactgccagg tcgttcatgt tcaggatgag cgcggccggc
     2521 tgtaccgagg tcattttttc agccacctca tcctgattcc ggatccgctc caaggtatac
     2581 ccctgcgggc gcaacgcgtc tcgcaccttc gtgtaaaaga aaatgtcggt cacggccact
     2641 acgatcgtgg tgttcattgc atcctgctag ggctagttca gggagctgat gaggcggccc
     2701 aaggccttct tggccaaggc ataatccggg ttgagagcca gggcttgctt gtaacagtcg
     2761 atggcttcgc tccacttgcc ttggcgttcg tacacgcggc ccaggttcaa gtgagggaat
     2821 gccgggcttt cgtagcgcgt ggcctgcatg gccttttgaa accagggaat cgcctcgtcc
     2881 aggtccccct tttcaatcaa ataggcgccg atgtcgttgt aaggattgcc gaagtgtgga
     2941 tcgcatgcga tggccttatg gcattcctca atcgcctcgt ccagtttgcc catgaagcta
     3001 taagtccagc cgagaaaggt ataggcttcg gccgtctgat gcgtggcgag cgagagtttg
     3061 tacagactga ccgcttcctc cagctgcccc ttcatctgta gttcgtaggc ttgctggaac
     3121 agttgccagg cttcgcgctt gtcttcctcg ctgccatcgc cttgtatgag aaagtcttgt
     3181 acatccatat gcggcttctg aaacaggttt ccggctgcgt tctcgggcgc tcactcaact
     3241 cgacgtacca acacagtacg tctcctcgat ttcgcttcct gcggccttgc tggaaacccg
     3301 tttgagaagc cgcaggacag tatgaactaa cgcttcttca gcttctctcg gattaactcg
     3361 gcgccgtacc ggcctgaaag taacatcgac ccaaatgccg ggcccatgcg tggcgtgccg
     3421 aacactgccg ccaccgccag tccgatcaca aagcaattgg gagaaacttc tccggttcga
     3481 tccatgacct cttcttccga tcgcgagacc cacatggcgc cattgccggg aacctgttga
     3541 tagagccccc gcttgtgcag cagattgacc accaccgcgt catgtccggt ggcgtcgacg
     3601 acaatcttac tctctaacgc gattggatca acatggataa tatcgtgtcc ggccatctcg
     3661 gcagtggtgc tattcaccac cactccttcg agcacccctt cgcggcgcag aatcaggtcc
     3721 acgacccgcg tcaggttcat gatcttcgcg ccggcgtcat aggcggccgc gatcaacgcg
     3781 ccagtcgcat gcggaggatc gaccatgtac atgccggggc attctttgat ctgcttgcag
     3841 ggtacaccca cctctttgag aatcttgtgc gccggagcgc agatggtagc tttgttcatc
     3901 aagtagccgc cggaccagaa ccctccgccc aaggccaggc tctgttcgac gaccagggtt
     3961 ttgaccccca tcctggccag atcgtgggcg cagatcaagc cagaagggcc ggcaccgacg
     4021 atgatgacgt cgctctcgat cagttgatcg aactcgttgt aatattcccg cgcaatctga
     4081 cgggtgatgt cgcgctcgcg tagtggtgcc ggttttggct tccccatgct acttttcctc
     4141 tcagcggctg ctgaacatga ctcccggctt cgttctcggc tccatcaaat cctcaacgta
     4201 cccctgaggg tacgcttccg gtttgagttc gcctgcggcc ttgccggata gtcatgttga
     4261 tcagccttac aggtaaattt cggaaccggc ctttttgaat tcttctgatt tttccttcat
     4321 gccgatctgg atggcctgct gctcctccag ttgtttctgc gccgcatagt cgcgaacatc
     4381 ctgcgtgatt ttcatggagc agaaatgcgg cccgcacatg ctgcagaaat gggcgacctt
     4441 cgccgcattg tc
//
LOCUS       DAOQEW010000269         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8366_length_4449_cov_9.310423, whole genome shotgun sequence.
ACCESSION   DAOQEW010000269 DAOQEW010000000
VERSION     DAOQEW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8366_length_4449_cov_9.310423"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLS35_18240"
     CDS             <1..576
                     /locus_tag="PLS35_18240"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNI20666.1"
                     /translation="SYLTDFESLAIKQVCFIEDLLVEELTDILVGLEQRISAYRNRYQ
                     ELEKKRRRLDDEIAMIKKYLELAETLYRVEADKAKLASLSSQIITDEKGIRPLPVTDV
                     TDQSREILLGKSKYVGKSVPEAAYQILREASRPMHAKELLQRLLEGGLQIKGKTPLTS
                     VATSLKRDKRFQKVGPNTFAVTTQQELTAVV"
     gene            complement(824..1750)
                     /gene="lpxC"
                     /locus_tag="PLS35_18245"
     CDS             complement(824..1750)
                     /gene="lpxC"
                     /locus_tag="PLS35_18245"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNI20667.1"
                     /translation="MRFQQTIGSPVSCSGVGLHSGQPVTLTLRPAPPNTGIVFIYRHD
                     PEETLIPASISNKVPTELCTAISVNGHQVKTIEHLLAALVGMEVDNVYAEVNAGEVPV
                     LDGSSSPFVRLIRAAGVIPQTRRQSYVKIMQPIEVVDGSKRVRIEPSSTPKITYSIHY
                     DHPLIQTQTYTYTCSAQAFEQDIAQARTFGFLHEVEALWSRGLGKGGTLDNTIVLSKE
                     GVVNESGLRFSNEFVRHKVLDLIGDIALLGFPFIGHVVAERSGHAMHTKLVEQILLQR
                     DKWALITGEHAVAASESRPSLGLLRPAPSLAI"
     gene            complement(2036..2707)
                     /locus_tag="PLS35_18250"
     CDS             complement(2036..2707)
                     /locus_tag="PLS35_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20668.1"
                     /translation="MEKIKVLIADDHRVVREGLAAILKTKEDINVVGEAQDGVEAVEK
                     TKTLMPDVILMDVSMPRMGGIEATRQIKREFPHMGIVALTMYEEQQYIFDLVRAGATG
                     YLLKDSESSQIVAAIRAIYRGESLIHPSVASKILAEFSLMSQKKGKKPAWVEHDLTER
                     EITVLRLVADGKTNKEIANSLDLSEKTVKNHVRNIFHKLQVYDRTQAAILAIRKGLIE
                     LEPRP"
     gene            complement(2694..3815)
                     /locus_tag="PLS35_18255"
     CDS             complement(2694..3815)
                     /locus_tag="PLS35_18255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNI20669.1"
                     /translation="MATTKTSQERRTTRRPTKRRSPGATHVVIIGAGRGGTALMEIFA
                     NDPLVRIVGVADISPEAPGLGLAKRLHIRVTRNYRQLLKMGPVDLVIDVSGNPEVGEY
                     LQDIRRMGVSVIGGASAKFMWQLIEARIRATADIEKALNKYQSLYRLYVKETGAAVTE
                     ERTRIACEIHDGLVQSLAGVNFKLELSQELLRKNPQASLATIRESKAQLKLAIQEARQ
                     VIFNLRPLHYDKMELIPALTNYLKSYETQYHIKTAFSVTGDETILFPRTKIFLFRIVQ
                     EALSNVQKHAKAGRVSVQLDIRLDLLQVTISDNGIGFDMDAVLRDPEKWDHFGIRGIL
                     ERARLVGGEATIDSKKGRGTRIVLRIPLADKETIRHGKN"
     gene            complement(3923..4387)
                     /gene="nrdR"
                     /locus_tag="PLS35_18260"
     CDS             complement(3923..4387)
                     /gene="nrdR"
                     /locus_tag="PLS35_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNI20670.1"
                     /translation="MKCPFCDDVEDKVVDSRMAKEGEVIRRRRECLSCKRRYTTYERV
                     EETMPAVVKKDGRREPFDRSKIVSGLKKACEKRPISTATIETVTDRIEKRIQDMGETE
                     IVSTAVGEEVMKELSQLDQVAYVRFASVYREFKDIDQFMDEIKSLAQQRRER"
BASE COUNT          957 a         1146 c         1289 g         1057 t
ORIGIN      
        1 agttacttga cagattttga atcacttgct ataaagcaag tatgctttat tgaggacttg
       61 cttgtggaag aattgaccga catcctggtg ggtctggagc aacggatcag cgcctaccgg
      121 aatcgctacc aggaacttga gaaaaagcga cgtcggctcg acgatgaaat cgcgatgatc
      181 aagaagtatc tggaactcgc cgaaacgctc taccgtgtgg aagcagataa ggccaagttg
      241 gccagcctct cgagtcagat tatcaccgac gaaaaaggga ttcgcccgct tcctgtgacg
      301 gatgtcacgg accagtcgcg cgagatcttg cttggaaaaa gcaagtatgt cgggaagagt
      361 gtgccggagg ctgcgtatca aatacttcga gaggcgagcc ggccgatgca tgcgaaggag
      421 ctgttgcaac ggcttttgga gggtggattg cagatcaagg gcaagacgcc attaacctcc
      481 gtcgccacct cgttgaaacg ggataagcgt ttccagaaag tcggacccaa tacgtttgcg
      541 gtcacgacgc agcaggaatt gacagcggta gtgtaagtgg gccgaggaag ttcatatcaa
      601 ctttaatgtg tgaagggagg tgagagtctt ggcaacgaag aaggcagcga agaaaccagc
      661 gaagaaggca gcgaagaaga agtaagtcct gccaggattt acacgccggg ggtaaggcca
      721 cttgcccccg gcgttttttt atgcccaaaa aatgaaagat gcttcgatca ccgcctggag
      781 ccggcggatc gcacgcgtgg tgaagccatg ctgagagaag cagttagatt gcgagggacg
      841 gcgcggggcg aagcagcccg agggaagggc gcgattctga tgcagcaacc gcatgttccc
      901 cggtgatcag cgcccatttg tcgcgctgga ggagaatctg ttcgacgagt ttcgtatgca
      961 tggcatggcc tgatcgttcg gccacaacgt ggccgatgaa cgggaagccg agcagcgcaa
     1021 tgtcaccaat gaggtccagc accttatgcc ggacaaattc attcgagaaa cgcaggcctg
     1081 attcgttcac cacgccttct ttggacagca cgatggtgtt gtccaaggtc ccgccctttc
     1141 caagtcctct ggaccagaga gcttcgactt cgtgcaggaa gccgaacgtg cgtgcctgcg
     1201 cgatatcttg ctcaaacgcc tgagcggagc aggtgtaggt gtaggtttgc gtttggatca
     1261 aggggtgatc gtaatggatg gaatacgtaa ttttcggcgt tgacgacggt tcaatcctca
     1321 cccgtttcga tccgtccacc acttcgatgg gttgcataat cttgacatac gattgccgcc
     1381 gagtctgtgg gatgacgcca gccgcgcgga tcaaccgaac aaacggactt gagctcccat
     1441 cgaggacggg gacttcccct gcattcacct cggcgtagac gttgtcgact tccattccca
     1501 ccagggcggc gaggaggtgt tcgatcgttt tgacctggtg tccattgacg ctgatggcgg
     1561 tgcagagttc cgtcgggacc ttattcgaga tcgatgcagg gatgagtgtt tcttctgggt
     1621 catgccgata gatgaagacg atgccggtat ttggaggggc cgggcgaagt gtcagtgtga
     1681 ccggctggcc ggaatgaaga ccaacgcctg aacatgaaac cggtgagccg atcgtttgct
     1741 gaaaccgcac ggtgcctcct tcgtaaaatc cagcactact tctagcaatg cttgtgccaa
     1801 atgcgaataa atgtataagt cattgattta ttggaaatga gtgcagtggt ttgcaaatag
     1861 gtctgttgct aaaaaaacac aattgtatct tttgttgaaa atgaaaaggt gcgaaagtaa
     1921 cgctttttca aagagttatc ggctgtgtgt cctgaaagat gcgggcaacg tagaacgaag
     1981 ggtccacgaa ctccgggaga gacagcccca gtactcggga taagcgagca cctccttagg
     2041 gtcttggctc caactcaatg agtccctttc gaatcgccaa aatcgcggct tgcgtgcggt
     2101 catacacctg aagtttgtga aaaatgttgc ggacgtggtt cttgaccgtc ttttcactga
     2161 ggtccaggct gttggcaatt tccttattcg tttttccgtc tgcgaccaag cggaggacgg
     2221 tgatttcccg ttcggtcagg tcatgttcaa cccaggccgg ctttttgcct tttttctgag
     2281 acatcagtga gaactcggcc aggatcttgc tggcgaccga aggatggatg agcgattccc
     2341 cgcgataaat ggctcggatt gctgccacaa tttgagacga ttcggaatct ttcagcagat
     2401 atcccgttgc ccccgcccgt acgagatcga aaatatattg ctgctcttca tacatggtca
     2461 gtgcgacgat tcccatgtga gggaattcgc gtttgatttg ccgcgtggct tcgatgccgc
     2521 ccatgcgcgg catgctgaca tccatgagga tgacatccgg catgagggtc ttggtttttt
     2581 ctacggcttc gacaccgtct tgggcttcgc cgacgacgtt gatgtcttcc ttcgtcttca
     2641 gaatagccgc cagcccttca cgaactacgc ggtgatcatc ggcgatcagg accttaattt
     2701 tttccatgac gaatcgtctc cttgtcggct agcgggatgc gcaacacaat ccgcgtgccg
     2761 cgtccctttt tcgaatcgat cgtggcctca ccgcccacca gtcgcgctcg ttccaagatg
     2821 ccacgaatgc cgaagtggtc ccatttttca ggatcgcgga gcactgcgtc catgtcgaac
     2881 ccgatcccgt tatcggaaat ggtcacttgt aacaggtcga ggcgaatgtc gagttgaacc
     2941 gacacccggc ccgccttggc gtgcttctgc acgttgctca acgcttcttg gacgatccgg
     3001 aataagaaaa tcttcgtgcg cgggaacagg atcgtctcgt ctccggtcac agaaaaggct
     3061 gtcttgatat ggtactgcgt ttcgtaggac ttcaaatagt tcgtgagggc cgggatgagc
     3121 tccattttat cgtagtggag cggccgtaga ttgaagatga cctgccgcgc ttcttgaatc
     3181 gctagcttca gctgagcttt ggattcacgg atcgtcgcca aactggcttg cgggtttttg
     3241 cgaagcagtt cctgcgagag ctccaacttg aaattgacgc cggcgaggct ttgcaccagg
     3301 ccatcgtgaa tctcgcacgc gatgcgtgtt cgttcttctg tcaccgccgc gccggtctct
     3361 ttcacgtaaa gccggtacaa cgattggtat ttgttcaagg ctttctcaat atccgccgtg
     3421 gcgcggatgc gggcttcgat cagttgccac atgaatttgg cgctggctcc gccgatgact
     3481 gaaacgccca tgcgacggat gtcctggaga tattcgccaa cctcggggtt gcccgacaca
     3541 tcgatcacca gatccacggg gcccattttg agcaattgcc gataattgcg ggtgacgcga
     3601 atatgaaggc gtttggcgag ccccagcccg ggcgcttccg gactgatatc agcgacgccg
     3661 acgatgcgta ccagcgggtc gttggcgaaa atttccatca aggccgtgcc cccgcgcccc
     3721 gcgccgatga tgacgacgtg cgtagcgccc ggtgatcgcc gtttggtcgg gcggcgggtc
     3781 gttcgtcgtt cttgtgatgt tttggttgtg gccatatcag tcagacaatc cgatggacgg
     3841 cggttctcct ctgttggcac ctatcctgcc tgggtctctt cacacggact ctgatccgga
     3901 acagcgggaa ggaggggagg gcctaacgct cccggcgctg ctgggccagg gatttgattt
     3961 catccatgaa ctgatcgatg tctttaaact ctcgatacac cgacgcgaat cgaacgtagg
     4021 ccacctgatc gagttgagac agctctttca tcacttcttc gccgacggcg gtgctgacga
     4081 tctcggtttc gcccatatcc tggatgcgtt tttcgatgcg gtcggtgacg gtttcgatgg
     4141 tcgcggtgct gatcggtcgc ttttcacagg cctttttgag tccggacacg atcttgcttc
     4201 gatcgaatgg ttcccggcgg ccgtcctttt tcacgacggc gggcatggtc tcttcgacac
     4261 gttcgtaggt agtgtaacgg cgtttgcacg agaggcattc acgccgccgg cggatgacct
     4321 cgccttcttt ggccatccgc gaatcgacga ctttgtcttc gacatcgtcg cagaaaggac
     4381 atttcacggg cggccgtcct gcctaagcgg gagacgaatc gtaggcatga aagatgggga
     4441 aacggttgc
//
LOCUS       DAOQEW010000270         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8383_length_4445_cov_7.471526, whole genome shotgun sequence.
ACCESSION   DAOQEW010000270 DAOQEW010000000
VERSION     DAOQEW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8383_length_4445_cov_7.471526"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PLS35_18265"
     CDS             complement(<1..1585)
                     /locus_tag="PLS35_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20671.1"
                     /translation="MSGTAGTFEQIARGMIDAMAPLRHALSSPDAFRSFMLRLGWETI
                     SVPPAYTALSTTIADAMQAVESLKDDPTAEEVLALLRKAQATYVAIQGIDQAPAGVDA
                     STFLSEMGERLFELLLTDYLAAHQPALFNLLSILNVIETEQIAATANQRAHVRTHFHW
                     ANIPEIIRDPASLPQRVYGWGTQELKFQLILQHFSELLFAFGFPVFIGKAEELLANGY
                     AGEDIDLDEETRWLKVPFYYITIADRDLEAAISVVELKGNGTTLPGLAIQPHIPSEFP
                     LTLHLAPTIDFRLRAGTNAATQVGIVLRPGDASIKYPFEPGTTPPSAGIGLGFDFKPS
                     SPTLLFGSPNATRLELQGASVDLGASFVQNELDVQVAAQLKGLTLVLQPGEADSFLRN
                     LFGDSERRLTIPLGFEWSRRNGVHFAGSDAFETSLPANLSLGPVTVQGLTVQLFAPTD
                     HSADLTLGVGAQISGTLGPVSFAVEGMGFRLQTTFAPGNAGPFDLSLGFKPPTGLGLA
                     ISTPGVTGGGFLGFDPQRAE"
     gene            complement(1582..4155)
                     /locus_tag="PLS35_18270"
     CDS             complement(1582..4155)
                     /locus_tag="PLS35_18270"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNI20672.1"
                     /translation="MPITGIQWKSNREYDIHLLRGRTLSPLLAQVNVELPDGNTQDAA
                     TYLAANADVTIDFQPSFRNVLDVTATPPTCTGFGITINNTNGEVRVPSPPTPAPVIHN
                     FLLHATAQDSSDGKEYRISVRIHLHNRVTSAWLTPPILTLRPDGPTLPQTTFRRFTVR
                     AQFDDNTVGDVTNQQGLTWGPLGNVEPSGRLVISVGNSPSDPAVEITATLPADLRDPG
                     NPTPPDIVARGHIRFAADWASESTIKTETVQVQDTWPGTINPELVPNFLFLSDGYLAE
                     DKPQFESQIRSLLGLMKKSRLTRPFDLLSTSMNYFQAFVPSSHHGISVLCEVYPAQKK
                     SGEVQTNSDDTVNLYCIPDPSDPAAGARWDLSNLLFRLGLPVPGQGLDRPVKEIRDYW
                     DSILDAVPHDRISNETVRRWQKLARRTFLEETDSTFGLAYGDYPNVTNESDSREVGFH
                     PRRMSRERLDTILNRLHDSHGNPMGQLWAARADGTRPNSYPLIFLFSSLKWDRGVNYG
                     RSYIAMNVEERYEIPARPVSGKPTYGIDLTGRITKNISHGRLIRGCHEVAHSFGLGDE
                     YSEKGTLPQSREIDRFYGNLQKHNDLLDSFKDIDGDLIKWRWHRIRKATVLMGAISEG
                     PSGVFRIPIPLGQSRQFKQGNTVLLRARRYPIPLPRNPDTSEHLQIVGLADPGGVADL
                     SKPEGPDNPLGAAIMVSPKSGHSFTAADAARFGAGCVLYLPVEASESARSEDYPFAEL
                     IALNVKDHITDRGCALNQDPDSDEICVPDKNDIQKPKKLDIDFPRCFKHKNRIVGLFT
                     GGKTFHCGIYHPTGNCIMRNSDSDGKEFCPVCRYLLVDIIDPHQHFSIDLDYGEIYPQ
                     K"
BASE COUNT          856 a         1206 c         1455 g          928 t
ORIGIN      
        1 attcggcccg ctggggatcg aagcccagga acccgccgcc ggttaccccc ggcgtactga
       61 tcgccaggcc gaggcccgtc ggcggtttga agccgaggga gaggtcgaac ggaccagcgt
      121 tgcccggagc aaaggtcgtt tgaagacgga accccatgcc ttctaccgca aacgagacgg
      181 ggccgagtgt gcccgagatc tgcgccccca ctccaagggt caggtccgcg gaatggtcgg
      241 tcggcgcaaa gagttggacc gtcagccctt ggaccgtgac gggtccaagc gaaagattcg
      301 cgggcaagga tgtctcgaag gcgtcgcttc ctgcgaagtg cacgccgttg cgtcgagacc
      361 attcgaaacc gagcggaatc gtgagccggc gttcgctatc gccgaacagg ttgcgcagga
      421 aggaatcggc ctcacccggt tgcaagacaa gcgtcaggcc tttgagttgc gcggctacct
      481 ggacgtccaa ttcattctgg acgaaggagg ctcccagatc gaccgatgct ccttggagtt
      541 caagacgggt ggcgttcggt gaaccgaaca gcagggtcgg ggacgatggt ttgaaatcga
      601 acccgagccc gatgccggcc gaaggcggtg tggtacccgg ttcaaatgga tacttgattg
      661 acgcgtcgcc ggggcggagc acgatgccaa cttgagttgc ggcgttggtg ccggcacgca
      721 ggcggaaatc gatggtcggc gccaggtgca aggtcagcgg gaattcgctc ggaatgtggg
      781 gttggatggc caggccgggc agggtggtgc cgttgccttt cagttctacc acactgatgg
      841 cagcttcgag gtcccggtcg gcgatcgtaa tgtaatagaa gggaaccttg agccatcgcg
      901 tctcttcatc caggtcgatg tcctcacctg catatccgtt ggccagcaac tcctccgcct
      961 tgccgatgaa gacggggaaa ccgaatgcga acaacagttc gctgaagtgc tggagaatga
     1021 gctggaactt gagctcctgc gtgccccatc cgtacacccg ttgcggaagg gatgcgggat
     1081 cgcgaatgat ctccgggata ttggcccaat ggaagtgtgt gcgcacgtgc gcgcgctgat
     1141 tggccgtggc cgcgatctgc tccgtctcga tcacattgag gatagagagc agattgaaca
     1201 gcgccggttg atgcgcggcg agatagtccg tcaggaggag ttcgaacagt cgttcgccca
     1261 tctcgctcag aaaggtgctc gcgtccacgc cggccggagc ctgatcgata ccctggatgg
     1321 cgacgtaggt cgcttgggct tttctgagca gggccagcac ctcctccgct gtcggatcat
     1381 ctttgaggga ttccactgcc tgcatggcgt cggcgatcgt cgtactgagc gcggtatagg
     1441 ccgggggaac ggagatggtt tcccagccga ggcggagcat gaaggaccgg aaggcatcgg
     1501 gactgctcag ggcatgccgc aacggcgcca tcgcgtcgat catgccgcgt gcaatctgtt
     1561 cgaatgtgcc ggccgtgcca ctcatttctg cgggtaaatc tctccgtagt ccaggtcgat
     1621 ggaaaaatgc tggtggggat cgatgatatc caccagcagg taacggcaca cggggcagaa
     1681 ctcttttccg tcgctgtccg agttacgcat gatgcagttg ccagtgggat gataaatccc
     1741 gcaatggaac gtctttcctc ccgtgaacaa gccgacgatc cggtttttgt gtttgaagca
     1801 gcggggaaaa tcgatatcga gctttttcgg cttctgaatg tcgttcttgt ccggcacgca
     1861 gatctcatca ctgtcaggat cctggttgag cgcgcagccg cggtccgtga tgtgatcctt
     1921 gacgttcagg gcgatcaact cggcaaaagg gtagtcttcc gatcgggccg attcactggc
     1981 ctccaccggc aggtagagga cgcaaccggc cccgaaccgg gccgcatcgg cggcggtaaa
     2041 tgagtggccg gatttgggag agaccatgat ggccgcgccc aagggattgt ccggaccttc
     2101 cggttttgag agatcagcca ctccgcccgg gtccgctagt ccgacgatct gcaggtgttc
     2161 gctggtgtcc ggattgcgcg ggaggggaat gggataccgt cgagcacgca ggagtactgt
     2221 gttgccctgt ttgaactggc ggctctggcc taatgggatc ggaatacgga agacgccgga
     2281 gggtccttcg ctgatggccc ccatgagcac ggtggctttg cggatgcgat gccagcgcca
     2341 tttgatgaga tcgccgtcga tgtctttgaa agaatcgagc aggtcgttgt gtttctggag
     2401 gttgccgtag aaccggtcga tttcacggct ttggggcaac gtgccctttt cgctgtattc
     2461 gtcgcccagg ccgaaggaat gcgccacctc gtggcaaccg cggatgaggc ggccatgcga
     2521 gatgttcttg gtgatgcggc ccgtgagatc gatgccatac gtcggtttcc cggagacggg
     2581 cctcgcaggg atttcgtagc gctcttcgac attcatggcg atataggagc gcccgtaatt
     2641 tacgccgcga tcccatttga gagaggagaa gaggaagatc agcgggtagc tgttgggacg
     2701 cgtgccatcc gctcgtgcgg cccagagttg gcccatggga ttgccatggc tgtcgtgcag
     2761 gcggttcaag atcgtatcca gccgctcccg agacattcgt cgcggatgaa agccgacttc
     2821 gcgactgtcg ctttcattgg tgacgttggg gtaatcgccg taggccaatc cgaacgttga
     2881 atcagtctct tcgaggaacg tccgtcgtgc caatttttgc cagcgtcgga cggtctcatt
     2941 ggaaatcctg tcatggggca cagcgtcgag gatgctatcc cagtagtcgc ggatctcttt
     3001 cacggggcga tcgagcccct gcccgggaac ggggaggccg agcctgaaca gcaggttgct
     3061 gagatcccag cgtgcccctg cggcggggtc tgagggatca gggatgcaat agagattcac
     3121 ggtgtcgtcg ctattggttt gcacctcccc gcttttcttc tgcgccgggt acacctcaca
     3181 aagcaccgaa atcccatggt ggctggacgg cacgaaggcc tgaaaataat tcatcgacgt
     3241 ggagagcaag tcgaacgggc gcgtcagcct gctctttttc atcaacccga gcaggctacg
     3301 gatttgggat tcgaactgtg gtttgtcctc ggcgagatag ccatcggaca agaacagaaa
     3361 gttcgggacg agttcggggt tgatcgtgcc aggccaggtg tcctgcacct ggacggtctc
     3421 cgttttgatt gtgctttccg acgcccaatc ggctgcgaac cggatatggc cgcgcgctac
     3481 gatgtccggt ggtgtgggat tgccgggatc ccgtagatcg gctggaagcg tggcggtgat
     3541 ttcgacggcg gggtcgctgg ggctgtttcc gaccgaaatg accaatcggc cggatggctc
     3601 cacgttgccc aatgggcccc atgtcagtcc ctgctgattg gtcacgtcgc cgacggtgtt
     3661 gtcatcgaac tgtgcgcgca ccgtgaatcg tctgaacgtg gtttgcggca aggtcggacc
     3721 gtccggccgc aacgtcagga tcggcggcgt cagccaggcg gaagtcacac ggttatgaag
     3781 gtggatccgg acgctgatgc ggtattcctt cccgtcgctc gaatcctgcg ccgtggcgtg
     3841 cagcagaaaa ttatgaatga ccggcgctgg agtaggaggc gacgggacac ggacttcacc
     3901 gttggtattg ttgatggtga tgccgaagcc ggtgcaggtc ggcggtgtgg cggtcacatc
     3961 gaggacgttg cgaaacgaag gctggaaatc gatggtgaca tcggcattgg ccgctaaata
     4021 ggttgctgca tcttgcgtgt ttccatccgg gagttcgaca ttcacttgag ccagaagggg
     4081 agagagggtc ctgccgcgca gcaggtggat gtcgtactct cgattgcttt tccattggat
     4141 tccggtaatc ggcatggtga ctagtgctgt ctggtgatct ctgaacggtg acggaataag
     4201 aaaatacgtt gcctcgcacg acgtcgtcga acaggatcag acacgatcat gaagggatga
     4261 gccgaaaggt actccgctcg tgatggcctg tcaaactcgt ctctgcgcaa atacctgcat
     4321 tgtgatgacg cagtctgcga cacaccatgt ctatgcgact accgctgtcg tccctcgccg
     4381 tttgcgcgcc ggagcctgcc cggctgcgcc gaagcgggcg acccgctcca acaccgccgc
     4441 cgaga
//
LOCUS       DAOQEW010000271         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8428_length_4432_cov_7.482294, whole genome shotgun sequence.
ACCESSION   DAOQEW010000271 DAOQEW010000000
VERSION     DAOQEW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_4432_cov_7.482294"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLS35_18275"
     CDS             <1..287
                     /locus_tag="PLS35_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20673.1"
                     /translation="VHEIGRWHDVHARGILVMALDSAKKRMAGILDVMLIPDAVLLGS
                     DIWAMIGQYDFFDVVGISPGDAVDFILRISRARASDGVVVRSRDFVARVV"
     gene            284..613
                     /locus_tag="PLS35_18280"
     CDS             284..613
                     /locus_tag="PLS35_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20674.1"
                     /translation="MMGFLRKGSGYGIRQVFDPVERMWTFNTPEPLTKDMESAVIDFV
                     RHVQNGRKLDVARVIGPGPRYDALLDSIKALDEGRYRVVVSNRDEVFVFKQTVGTGKA
                     FSLPIGG"
     gene            616..1131
                     /locus_tag="PLS35_18285"
     CDS             616..1131
                     /locus_tag="PLS35_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20675.1"
                     /translation="MAQTFNLHCAGVAFAATKNMAAVLNQHATEILKIREAGLSNAQT
                     AAVTGVVCQMESRFYTGTATLTTPTAVTPVALDTSNTAPTTATYGHAGTIGGTANTLD
                     TWIWSSDEATASTATSDELECFLPLNKVYQWIPHSDIQPITLRQDKMFMLYNVSGAAG
                     LLHSRILFTKE"
     gene            1135..1326
                     /locus_tag="PLS35_18290"
     CDS             1135..1326
                     /locus_tag="PLS35_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20676.1"
                     /translation="MSTLTEIWNQEPRIITNVARRPNGPYVSLAHENYEDALNAARKA
                     QVVTIADAVANDWSVAEAP"
     gene            1326..2573
                     /locus_tag="PLS35_18295"
     CDS             1326..2573
                     /locus_tag="PLS35_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20677.1"
                     /translation="METYRLHDEVKEWAFSIEMPTTAVTNHPYLTHVATLALFNKTGS
                     GKMVRVRQIKAERLLAQTTNTAIGTKLALYRITAHTASEEPMTAYSMDTNNASLPSQV
                     EMYRRPFSVTTSGTPIDSTCAVPCANEARALGHMVSRMAGAQEYQDVYVWGSDTGNEV
                     QKVVLREGEGISLSTDGVTNAFPHWLEASVTVRNSSSGACYRYNFPTYFTPLPVFSLL
                     NGSGSGVVLEVVRVGLREIGTDVLPQVTVERIDGIDETSGYSSTPDPFDTANSLDANI
                     IGRGNVKVTMDGSKRGAFMVIPHLAYKIGAGLGVGPNLSGFHPQNQDSHHTSQPFAGR
                     YPHEDLHDICLREGEGIGIFQRSASGQIAFDLMIQFTVEGVTGGYPPESKVENNYVFG
                     PNDEYTGSLAGGSTYSRSRVVNR"
     gene            2570..4048
                     /locus_tag="PLS35_18300"
     CDS             2570..4048
                     /locus_tag="PLS35_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20678.1"
                     /translation="MSLLKQSTAREKMVFMTDSSDHVTGKTGLTLTITASKNGAAFAS
                     ITPTVTERGNGWYSLSLTSSHTDTLGDLALHVTGTAADPSDLVWEVVSLLPGDAVAIQ
                     TGTGSGQLNISSGVVESNLKQIDGLTTNGNNATLNLKQLNVANSSGSAIIATSSGGNG
                     HGITATGDGTGHGIKAAGSASGTGDGINATGGLSGATNGIVGQANSTGSGIVGRGVST
                     GGAGVLADGGTGGSGMSILGGTFTGDALVVTGRGSFGASKGAVFTGSGGGAEIDANIT
                     GNIIGNVTGSVAEATTIGAGGITSTTMDNGALASIRNKFLVLQDTLNGPTVTATTVTL
                     NSSNAANDDAYNGMLLANFNASGELKQVRTITDYDNATVTVTVDRPWTSNPADTDQIR
                     IYEFGIGLPRINPLKGVALNNFEFVMYDSTNHAPATGLTVTAQRSIDGGAYAACANAV
                     SEVSAGTYKISLASTDMAGNVIKLKFTATGADQQDITLVTQE"
     gene            4045..4236
                     /locus_tag="PLS35_18305"
     CDS             4045..4236
                     /locus_tag="PLS35_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20679.1"
                     /translation="MIKNWASKAGEFVSAIVSWLPQKFGTGTPVVSPGYAIAFSLEID
                     RDLSKTLYIKRFVNVEVEQ"
     gene            4236..>4432
                     /locus_tag="PLS35_18310"
     CDS             4236..>4432
                     /locus_tag="PLS35_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20680.1"
                     /translation="MAANEIHKGDIGTVFTVTVKEGSVVVDLSSSTAKQIKFRKRSGS
                     ILTKTASFVTDGTDGKIKYTT"
BASE COUNT         1004 a         1180 c         1331 g          917 t
ORIGIN      
        1 acgtccacga aatcggacga tggcacgacg ttcacgcgag gggaatactc gtaatggcgc
       61 tggattcagc gaagaagcgg atggcaggga ttctggacgt gatgttgatc ccggacgcgg
      121 tgttgctcgg tagcgacatt tgggcgatga tcggtcaata cgattttttt gacgttgtcg
      181 gcatttcacc tggcgatgcc gttgatttta ttctgcggat ttcgcgggcg cgggcttccg
      241 atggcgttgt cgtccgaagc cgtgactttg tggcgagggt ggtgtgatgg gctttttgcg
      301 aaaaggaagc ggctacggga tccggcaggt ttttgaccca gtggagcgaa tgtggacgtt
      361 taacacgccc gagccattaa ccaaggacat ggaatcggct gtgatcgact ttgtgcgcca
      421 tgtgcagaac ggaagaaagc ttgacgtggc gcgggtgatt gggcctgggc cgagatacga
      481 tgcgcttttg gattcaatca aggcattgga tgaggggaga taccgggtgg tggtatcgaa
      541 tcgtgatgaa gtttttgttt ttaaacagac agttggaacg ggcaaggctt tcagcctccc
      601 gataggaggc tgacaatggc acagacgttc aacttgcatt gcgcgggcgt ggcttttgcc
      661 gcaacgaaga atatggcggc ggttctgaac caacacgcaa ccgaaatcct aaagattcgt
      721 gaggccgggc tttcaaacgc gcagacggcg gccgttacgg gcgttgtctg ccaaatggaa
      781 tcccgattct atacggggac ggcgacgctg acgacaccga cagcggtaac accggttgcg
      841 ttggatacct ctaacacagc ccctacgacc gccacttacg gacacgccgg aactatcggc
      901 gggaccgcga acacgcttga cacttggatt tggtcttccg acgaggccac ggcgtcaacg
      961 gcaacttccg atgagctgga gtgtttcttg ccgctgaaca aagtctatca gtggatcccg
     1021 cactccgaca tccagcccat cacgttgcgg caggacaaga tgttcatgct ttacaacgtc
     1081 tctggcgctg ctgggcttct ccacagccgg attctattca ccaaggaata agagatgtcg
     1141 accttgacgg aaatctggaa ccaagagccg cggatcatca cgaacgtggc caggcgtcct
     1201 aatgggccct atgtttcgtt ggctcacgaa aactacgaag acgccctgaa cgcagcgcgc
     1261 aaggcccagg ttgtcaccat tgccgatgcc gtggctaatg attggtcggt ggctgaggcc
     1321 ccttgatgga gacttatcgt cttcacgatg aggtcaagga gtgggcattt tctattgaaa
     1381 tgcctacgac ggcggtgaca aaccacccct acttgacgca cgtggcgacg ttggctctat
     1441 tcaacaagac tgggagcggg aagatggttc gtgttcggca gatcaaggct gagcggttgt
     1501 tggcgcagac gacaaacacg gcgattggga cgaagttggc gctttatcgg atcacggcgc
     1561 acacggcctc ggaagagccg atgaccgcgt attcgatgga cactaacaat gcgtcgttgc
     1621 cgtcgcaggt ggaaatgtat cgtcgcccat tcagcgtgac aacgagtgga acaccgatcg
     1681 actccacatg cgcggtccct tgcgcgaacg aggccagagc gttgggccac atggtatctc
     1741 ggatggccgg agcccaggaa taccaggatg tttatgtttg ggggagcgac accgggaacg
     1801 aagtccagaa ggtcgttttg cgtgaagggg aaggaatttc tttgagcacg gatggagtca
     1861 caaatgcgtt tccgcattgg cttgaggcga gtgtaacggt gaggaattcg tcttcgggag
     1921 cgtgttatcg atacaacttc cccacctatt tcacgccttt gcctgttttt agcttgctga
     1981 acggatccgg ttccggagtg gtcttggaag ttgtcagggt gggattgcgt gaaataggga
     2041 ccgacgtttt gcctcaagtg acagtggagc ggatcgatgg gattgacgaa acgagcgggt
     2101 attcttcaac gccggacccg ttcgataccg cgaattcact ggatgctaac atcatcggac
     2161 gcggaaacgt aaaagtgacg atggacggct caaaacgcgg ggccttcatg gtgattccgc
     2221 acctggccta caagatcgga gctggccttg gcgttggccc gaatctaagc ggtttccatc
     2281 cacaaaacca agattctcac cacacgtcgc agcctttcgc cggcagatac ccgcacgagg
     2341 atttgcacga catctgtttg cgcgagggag aggggatcgg gattttccag aggtctgcga
     2401 gtggacaaat cgctttcgat ttaatgatcc agttcactgt cgaaggcgtc accggtggat
     2461 atccgccgga aagtaaggtc gagaacaact acgttttcgg accgaatgac gaatacacgg
     2521 gcagccttgc tggcgggagc acttacagcc gatcgcgggt ggttaaccga tgagccttct
     2581 taagcaatcg acggcgcggg agaagatggt ctttatgacc gactccagcg accacgtcac
     2641 cggaaaaacg gggctgacgc tgacgattac ggcgtccaag aacggggccg ctttcgcttc
     2701 catcaccccc accgtcaccg aacgtgggaa cggctggtat tcgttgtctc tgacctccag
     2761 ccacaccgac acccttggcg acctcgccct tcacgtcacc gggaccgctg ctgatccttc
     2821 ggacctggtt tgggaggtcg tgtctttgct ccccggggac gcggttgcga ttcagacagg
     2881 gactggatct gggcagttga acatcagttc cggtgtcgtg gaatcgaatc tgaagcaaat
     2941 tgacggttta acgaccaacg ggaacaacgc cacgcttaac ctgaagcagc tgaacgtggc
     3001 caacagttcc ggcagtgcca ttattgcaac gtccagtggt ggcaatggtc acgggataac
     3061 cgccaccgga gacggaaccg gtcacggaat caaagccgcc gggtctgcct ccgggactgg
     3121 ggacgggatc aacgccactg gcggtttgag cggcgccaca aatggaatcg tcggacaagc
     3181 aaacagcacc gggagcggta tcgttggtcg tggcgtaagc accggtggcg ctggtgtgtt
     3241 ggcggatggt gggacaggtg ggagcgggat gtcgattctc ggagggacat tcaccgggga
     3301 cgcattggtt gtgactgggc gagggagttt cggcgcatcg aaaggtgctg ttttcacggg
     3361 aagcggcggc ggcgcagaaa tcgatgccaa tatcaccggg aacatcattg ggaacgtcac
     3421 ggggtccgtt gccgaggcca ccacgatcgg ggccggcggg atcacgtcta cgacgatgga
     3481 caacggcgcc ctggcctcca ttcgcaacaa gttccttgtc ctgcaagaca cgctcaacgg
     3541 tccaacggtg acggcaacaa cggtgacact caacagttca aacgccgcca acgacgacgc
     3601 ctacaacggc atgttgcttg ccaatttcaa tgcctccggt gagctgaagc aggtgcggac
     3661 gattacggac tacgacaacg ccaccgtcac cgtcaccgtt gaccggccct ggacttcaaa
     3721 cccggccgac accgaccaaa ttcgcattta cgagttcgga atcgggcttc cgcgaatcaa
     3781 cccattgaag ggggtggctt tgaacaattt cgagttcgtg atgtatgaca gcaccaatca
     3841 cgccccagcc actgggctga cggtgacggc gcaacggagc attgacggcg gggcctacgc
     3901 cgcctgtgcc aacgccgtct ctgaggtcag cgccggcacc tacaagatca gcctcgcctc
     3961 caccgacatg gccgggaacg tgatcaagct caaattcacg gccactggcg ccgatcagca
     4021 ggacatcacc ttggtgaccc aagaatgatc aaaaactggg cctcaaaagc aggggaattc
     4081 gtttccgcca tcgtttcctg gcttccgcag aagttcggaa ccggcactcc cgtggtttcc
     4141 cctgggtatg ccatcgcttt ttcacttgag atcgaccgcg atctctcgaa aacactctat
     4201 atcaaacggt ttgtgaacgt ggaggtcgaa caataatggc tgccaacgag atccacaaag
     4261 gcgacatcgg aaccgtcttc acggtgacgg tcaaggaagg ctctgtcgtg gtagacctct
     4321 cctcgtccac cgcgaagcag atcaagtttc gcaagcgaag cggctcaatc ctcaccaaaa
     4381 ccgcctcttt tgtaaccgat ggcaccgatg gcaagatcaa atacaccacc at
//
LOCUS       DAOQEW010000272         4421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8464_length_4421_cov_10.409757, whole genome shotgun sequence.
ACCESSION   DAOQEW010000272 DAOQEW010000000
VERSION     DAOQEW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8464_length_4421_cov_10.409757"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2211
                     /locus_tag="PLS35_18315"
     CDS             <1..2211
                     /locus_tag="PLS35_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003117278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNI20681.1"
                     /translation="LMVALIHRMQVRARDQLAEMFLRRVATIHKRAKEELERIQIDQR
                     GQVERLVGTLDGVLSILADEPDNAAAGAQIREYLEPAGGVEQIRERCAQIQASSGNNY
                     LPLIWKHFRPHRSLLFRLAHLLDIRPSSQDRTLIDALEVIKLYQDKHRIDWIGDRVDL
                     SFAAERWRKLLRTGDTTGVHAGISRRHLEICVFSHLAEDLRAGDLSIAGSEEFADYRD
                     QLIPWEECQALLPAYCEKIGLPLKADDFVKGLRDALSKTALTLDERFPECHGDVVLNA
                     FGEPVLRKTTAREIPQSAVALQNALTERMPSRHILDVLGNIEQWVGFTRHFGPLSGNM
                     AKIKQPAERYLLTIFAMGCNLGPSQAARHLSNNGITPHMLSFVNRRHLSIESLEAAQR
                     ELNEVYLRLDLPKIWGDGKTVAADGTQYDFFDENLLAGYHFRYRKMGAVAYRHVANNY
                     IAVFRHFIAPGVWEAIYVIEGLLKAGLSVESDTVHSDTQGQSSTVFAFTHLLGIKLMP
                     RIRNWKNLTLYRPEKAAKYEHINRLFSGVVNWGLIERHWKDLMQVALSIYAGRISSAT
                     LLRKLGSSSRKNRLFFAAQELGNVVRTMFLLEWIGNREMRQEITANTNKVESYNGFAK
                     WVSFGGDVIAVNEPDEQQKRLRYNDLVASALILHNTVDMIRTLQRMKREGWKISDEEV
                     SFLSPYQVAHVKRFGVYSLKLNRKPEAWIGDTTFKEAAASVQYARQAKTKVESK"
     gene            2208..2564
                     /gene="tnpC"
                     /locus_tag="PLS35_18320"
     CDS             2208..2564
                     /gene="tnpC"
                     /locus_tag="PLS35_18320"
                     /inference="COORDINATES: protein motif:HMM:NF041282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase post-transcriptional
                     regulator TnpC"
                     /protein_id="HNI20682.1"
                     /translation="MKIPPPNFLTTPYGDVNAAKLVDLQDGYDTTELLRQVEHLEPIM
                     RRLKQNNGIRDDILRLHRMAHTVINGAPLSEPYKEDLWEAAAALVEELQSVARACCDI
                     AGAIQPLADLEPHLED"
     gene            complement(2582..3274)
                     /locus_tag="PLS35_18325"
     CDS             complement(2582..3274)
                     /locus_tag="PLS35_18325"
                     /inference="COORDINATES: protein motif:HMM:NF027613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNI20683.1"
                     /translation="MSTDDEDVRQPWVLILDTCVLLDIVRSPVRREFSPESARALVDV
                     SSRVRAQAQEFRIVIPSLVREEFELNLPKVQDDAVQELSRTLSNLSHAHQTLDILLAR
                     TPSPLTHDAAQWIADCVSFARDLINAAQELPTSDEDVRRAGMRSLKGVAPAKRGKSSL
                     PDCVITEFALRIARENVQNSGAPSVLFISSNDADYCDGRRLTASLQTEFDAAGLDFCR
                     NWAESRGSIIRR"
     gene            complement(3271..3936)
                     /locus_tag="PLS35_18330"
     CDS             complement(3271..3936)
                     /locus_tag="PLS35_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20684.1"
                     /translation="MDNDLGSFLVFRELTVRGLSPALERLGDVIQEHLPAHWLRDKAK
                     EQENTRMFGDRSLLVFERDGSGGLPASGVAMLTDGEKAEVVNIVPLEKSQLSVQEYNA
                     VLVELTTSAIQPTAVALGLKCELTDDLKPFTAWVSPAAGDALRRFSILANHGTGSAHP
                     LDRERWFAFLLHCHQDRCELDTDMLQRWLMEQGGWTFERASDLAIEYEFGRGLLKFAE
                     QNA"
     gene            complement(3942..>4421)
                     /locus_tag="PLS35_18335"
     CDS             complement(3942..>4421)
                     /locus_tag="PLS35_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20685.1"
                     /translation="WARASGWDVKDLMAYVGWKDIKSAMRYIDAVDSGLQARFEKGLI
                     LREVPAENKAAPAESRLASETATVRVKLTLIRLSESARGMNRARQAIEQHCFEPLNMR
                     RINADGSEYELAIPLVSRDALDETIYELIDTMYQLADDNHCGLETSFHEPATNTTWD"
BASE COUNT          950 a         1264 c         1274 g          933 t
ORIGIN      
        1 ctgatggttg ctctgatcca ccgcatgcag gttcgcgccc gggatcagct ggctgaaatg
       61 ttccttcgcc gcgtggccac catccacaaa cgcgcgaagg aagagttgga aaggatacag
      121 atcgaccagc gaggccaggt tgagcgcctt gtcggaacat tggacggtgt gttgtcgatt
      181 ctggcggacg agccagacaa tgccgctgcc ggcgcccaaa ttcgagagta tcttgagcct
      241 gcgggaggcg tcgaacagat tcgagagcgc tgtgctcaga tacaggccag tagcggcaat
      301 aattatctgc cgctgatctg gaagcacttc cggcctcatc gctcgttgct cttcaggctc
      361 gcacatcttc tcgatattcg tccgagcagc caagatcgaa cattgatcga cgcgcttgaa
      421 gtcatcaagc tgtatcagga caagcatcgc attgactgga tcggggaccg tgttgacctc
      481 tcgttcgctg cggagcgctg gcgcaagctt ctgcgcaccg gtgatacgac cggtgtccat
      541 gctggaatca gccgtcgtca cctggaaatc tgcgtgttct cacacctggc agaggatttg
      601 cgtgcggggg atctcagcat cgccggctcc gaggagttcg cggattaccg tgatcagttg
      661 ataccttggg aagaatgcca ggcactcctt cccgcctact gcgagaaaat cggacttccg
      721 ctcaaggccg acgactttgt gaagggacta cgcgacgcac tcagcaagac agcgctgacg
      781 ttggacgaga ggtttcccga atgccatggt gacgtggttc tgaatgcctt cggagagccg
      841 gtgctgcgta agacgactgc ccgcgaaatc ccgcaatccg cggtggcact gcagaatgcg
      901 ctgacggaac gcatgccctc acggcacatc cttgacgtgc tgggaaacat cgagcaatgg
      961 gtgggattca cccgacattt cggcccgctt tccggcaaca tggccaagat caagcagcct
     1021 gccgaacgct atctgctgac tatctttgcc atgggttgca acctgggtcc tagtcaggcc
     1081 gcgaggcatc tcagcaacaa cggcatcacg ccgcacatgc tctcgttcgt taatcgccgc
     1141 cacttgtcga tcgagagcct cgaagccgcg cagcgcgaac tcaacgaggt ctatcttcgg
     1201 ctggatctgc ccaagatctg gggggacggc aagaccgttg ccgccgacgg tacgcaatac
     1261 gatttcttcg atgagaatct gctggccgga taccattttc ggtatcgcaa gatgggcgct
     1321 gtggcttacc gccacgttgc caacaactac atcgcggtgt tccggcactt catcgcccct
     1381 ggtgtctggg aggccattta cgtcatcgaa gggttactca aggcgggcct gtcagtcgaa
     1441 tccgatacgg tgcattccga tactcagggg cagtcttcga cggttttcgc ctttacccat
     1501 cttctgggca tcaagctgat gcccagaatc cggaactgga agaatctgac gctctaccga
     1561 cctgaaaagg ccgcgaaata cgaacacatc aaccgcctgt tctccggtgt ggtgaactgg
     1621 gggttgattg agcgccactg gaaagacctg atgcaggttg cgctttccat ctacgcgggc
     1681 cggatctctt ccgccacgct gctccgtaag ctgggctcca gcagtcgcaa gaaccgtctt
     1741 ttcttcgctg ctcaagagtt ggggaatgtc gttcggacca tgttcctgtt ggagtggatc
     1801 ggcaaccggg agatgcgtca ggagatcacg gccaacacca acaaggtcga gtcctataac
     1861 ggctttgcaa aatgggtctc attcggtgga gacgtcatcg ccgtcaatga gccggacgag
     1921 cagcagaagc ggttgcgcta caacgatctc gtggcttcag ccctgattct ccataacact
     1981 gtcgacatga tccggacgct tcaacgcatg aagcgggagg gatggaagat ttcggacgag
     2041 gaagtgtcct tcctgagtcc ctaccaggtg gcgcacgtga agcgtttcgg ggtttacagc
     2101 ctcaaactca atcgcaagcc cgaagcctgg attggcgata ccacgttcaa agaggctgcg
     2161 gcctccgtcc aatatgcgcg gcaggcaaaa accaaggttg aatcaaaatg aaaataccgc
     2221 ctccgaactt tttaacaacg ccgtacggcg acgtgaacgc tgccaaactg gtcgatctgc
     2281 aagacggcta tgacacaact gagttgctga ggcaggttga acacctagag ccgatcatgc
     2341 ggcgcctcaa acagaacaat gggattagag acgacatcct gcgccttcac cgtatggccc
     2401 acacggtgat caacggtgct ccgcttagtg agccatacaa ggaggatttg tgggaggcgg
     2461 cagctgcgct ggtagaagag cttcagtcgg tggccagggc gtgctgcgac attgcaggcg
     2521 cgattcagcc cctcgcagat cttgaacccc acttagagga ttgatcggca tgggtcttca
     2581 cctacctgcg tatgatgctt ccgcgagact ccgcccagtt tctgcagaaa tccaggcctg
     2641 cagcatcgaa ttcagtctgt agcgatgcag tgaggcgtcg gccgtcacag tagtcagcat
     2701 cgttggagct gatgaaaagg actgagggcg cgccggagtt ctggacgttt tcccgggcga
     2761 tacgcagtgc gaattcggta atcacacaat ccggcaagct ggatttgccc cgcttcgcag
     2821 gcgcaacgcc tttcaaggag cgcatgcccg cacgtcggac atcttcgtcg gaggtcggga
     2881 gctcttgggc ggcattgatc aggtctcgcg caaagcttac gcaatccgcg atccattggg
     2941 ccgcgtcatg agtcaaaggg ctcggcgttc gggctaacag aatgtccagt gtctggtgtg
     3001 catgggagag attcgacagc gtccgggaaa gctcttgaac cgcgtcatcc tggacctttg
     3061 gtaggttcag ttcaaactct tcgcgaacta gcgaagggat gacgatacgg aattcctgag
     3121 cttgagcacg tacccggctg gagacatcga cgagtgctcg tgcactctct ggggagaatt
     3181 cgcgtcggac tggcgatcga acgatatcga gtaggacaca ggtatcgaga atgaggaccc
     3241 acggctgacg aacgtcctca tcgtcggtgc tcatgcgttc tgctcggcaa atttcaacag
     3301 gcctcgtccg aactcatatt cgatggccag atcacttgca cgctcgaagg tccagccgcc
     3361 ttgctccatc agccatcgct gcagcatgtc cgtgtccagt tcacatcggt cctgatggca
     3421 gtgcagaaga aaagcaaacc agcgctccct gtccagcggg tgggccgagc ctgttccatg
     3481 gttggccaga atgctgaagc gccgcaaggc gtcccctgcc gcggggctga cccatgccgt
     3541 aaaaggcttc aagtcgtcgg tgagttcgca tttgagcccc aatgccacag cagtaggttg
     3601 tatcgcgctc gtggtcagtt caaccaggac tgcgttgtac tcctggacgg acaactgact
     3661 tttttcgagc gggacgatgt tgacgacctc cgccttctcg ccgtcagtca gcatggcgac
     3721 cccggaagcg ggtagccctc cagaaccatc cctctcgaaa acaagcagcg atctatcgcc
     3781 gaacatgcgg gtattttcct gctccttggc cttgtcgcgt agccagtgcg cgggcaagtg
     3841 ctcttggatc acatctccca agcgctcaag ggccggagaa aggccgcgaa ccgtcagttc
     3901 ccgaaaaacg aggaaggagc ccaaatcgtt atccatgtga atcaatccca ggtggtgttg
     3961 gtcgcaggtt cgtgaaaact cgtttcgagt ccgcagtgat tgtcatccgc aagctgatac
     4021 atcgtgtcga tcaactcata gatcgtttcg tccagcgcat ctctggatac aaggggaatc
     4081 gccagttcgt actcggagcc gtccgcgttg atacggcgca tgttcaaggg ttcgaagcag
     4141 tgctgttcaa tggcttggcg ggcccggttc atgccgcgcg ctgactcgct cagacggatc
     4201 aacgtgagtt ttacgcggac agtggcagtc tcggacgcaa ggcgtgactc cgccggcgcg
     4261 gctttatttt cagcaggcac ctctcgcaag atcaacccct tctcgaagcg tgcctgcagc
     4321 ccggaatcca ccgcatcgat gtaacgcatg gccgacttga tgtccttcca gccgacatag
     4381 gccatcaagt ccttcacgtc ccaaccgctg gcccgggccc a
//
LOCUS       DAOQEW010000273         4333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8773_length_4333_cov_9.136746, whole genome shotgun sequence.
ACCESSION   DAOQEW010000273 DAOQEW010000000
VERSION     DAOQEW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_4333_cov_9.136746"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..492)
                     /gene="rpsK"
                     /locus_tag="PLS35_18340"
     CDS             complement(109..492)
                     /gene="rpsK"
                     /locus_tag="PLS35_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNI20686.1"
                     /translation="MSVKKGKKKERKIVQSGVAHVQASFNNTIVTITDMSGNTVVWAS
                     SGNQGFKGSRKSTPFAAQRAGEAAARKAMENGMRQVDVYVNGPGAGRESAIRSLQSAG
                     LRINLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(534..923)
                     /gene="rpsM"
                     /locus_tag="PLS35_18345"
     CDS             complement(534..923)
                     /gene="rpsM"
                     /locus_tag="PLS35_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNI20687.1"
                     /translation="MARIAGVDLPKDKRIEIGLTYVFGIGRVAAQAILKKAGVDGAIR
                     VKDVSEDKIVKIREVIERDYRVEGDLRKEVSMNIKRLVDTGTFRGLRHRKGLPVRGQR
                     TKTNARTRKGRRSGVGSKPQKPAGSRA"
     gene            complement(1058..1171)
                     /gene="rpmJ"
                     /locus_tag="PLS35_18350"
     CDS             complement(1058..1171)
                     /gene="rpmJ"
                     /locus_tag="PLS35_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNI20688.1"
                     /translation="MKVKSSVKPICAKCKVVRRRGVVRVLCANPRHKQRQG"
     gene            complement(1190..1408)
                     /gene="infA"
                     /locus_tag="PLS35_18355"
     CDS             complement(1190..1408)
                     /gene="infA"
                     /locus_tag="PLS35_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNI20689.1"
                     /translation="MPKEDVIEVTGTVAETLPNAMFRVELENGHRILAHISGKMRMHF
                     IRILPGDKVTIQLSPYDLTRGRITYRFK"
     gene            complement(1430..2185)
                     /gene="map"
                     /locus_tag="PLS35_18360"
     CDS             complement(1430..2185)
                     /gene="map"
                     /locus_tag="PLS35_18360"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNI20690.1"
                     /translation="MIVFKTPDEVTLMAQASRVVAEVLALLKGKVEPGITTEDLDRIA
                     EEAIRGRGAIPAFKGYRNYPKTLCASINEQVVHGIPSKRKLKEGDIIGLDLGAIVSGF
                     YGDSAVTVPVGSASEEALRLIQVTEEAMYQGIAKAVVGNRLSDVSHAIQTHVEQAGFA
                     VVTEFVGHGIGRQLHEEPQVPNYGKPGQGPRLQVGMVLAIEPMVNMGGSAVRILEDRW
                     TAVTQDGRWSAHFEHTIAIQPSGPAKILSQLSH"
     gene            complement(2182..2838)
                     /locus_tag="PLS35_18365"
     CDS             complement(2182..2838)
                     /locus_tag="PLS35_18365"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNI20691.1"
                     /translation="MRLVFLGAPGVGKGTQADRVTAQFGWPKISTGDLLREAVRNQTA
                     LGIEAKKSMDAGKLVPDSVVIGMVRETLAAPSCQKGFVLDGFPRTVPQAEELQALLTG
                     RNLKLDRVINFRVSRHDVVTRLSGRRSCPKCQSVFHIAFAPPKIEGQCDRCGASLIQR
                     SDDKPETIEARLKVYDEQTAPLIAYYQAKNLLSDLDGSGEMAAVYTRLSALLLGLGAQ
                     "
     gene            complement(2848..4161)
                     /gene="secY"
                     /locus_tag="PLS35_18370"
     CDS             complement(2848..4161)
                     /gene="secY"
                     /locus_tag="PLS35_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNI20692.1"
                     /translation="MFERLLTSFQNIFKIPELRTRVLFTLGMLIVYRVGAHIPTPGIN
                     GEALSEFLQKQGGALLGFLDIFSGGSLSRLTIFALGIMPYISASIILQLLTVVIPHLS
                     KLAKEGERGRKKIIQYTRFGTIVIALIQGFGIAVGLEQMNQGAFVLSPGWGFRFMTVI
                     TLCAGTGFLMWLGEQITERGIGNGISLIIFAGIVARLPAAVAQTFDLYKVGQLSFPLL
                     IVLAVVMFGVVAAIVFLESGRRKVPVQYAKRVIGRRVYGGQSTHIPLKINTAGVIPPI
                     FASSIIAFPATIAGFFETPWIKAIGSQLAPGSLLYTLMYVGLIVFFCFFYTAVVMNPV
                     DMADNLKKYGGFVPGIRPGQRTSDYIYSVLTKITFAGAIYLAIVCVIPEFLIYKLNMP
                     FYFGGTSLLIVIGVGLDTAQQIESHLLNRNYDGFLQKGKLRGRTS"
     gene            complement(4154..>4333)
                     /locus_tag="PLS35_18375"
     CDS             complement(4154..>4333)
                     /locus_tag="PLS35_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL15m family ribosomal protein"
                     /protein_id="HNI20693.1"
                     /translation="ITPQLLMNEGLVRRKGELIKILAQGELTKPLVVQAHKFSKSAEA
                     KIQAAGGRAEVIPCV"
BASE COUNT         1018 a         1263 c         1046 g         1006 t
ORIGIN      
        1 gcagacaggt ccactatatt ttgccacgct actacccctc ctctacctca acaacttaag
       61 tacaaccgac ttagatattt cgttactgcg aagatgcccg gatctctcct acacccgccg
      121 acgtttcggc ggccggcatc cgttgtgagg aatcggcgtc acgtcgcgga tcaaattgat
      181 acgcaatccg gcactttgca atgagcgaat ggcggactcg cgtccagcac ccggcccatt
      241 gacatagaca tctacctggc gcatgccgtt ttccatggct tttctggctg cagcttcacc
      301 cgctcgttgc gcggcgaatg gagtactctt gcgtgacccc ttaaacccct gattgcctga
      361 actggcccac accacggtgt tgccgctcat atccgtaatg gtcacgatcg tattattaaa
      421 cgaggcctga acgtgagcca ctccgctctg aacaatcttc cgttccttct tcttgccttt
      481 cttcacactc atagaatctc cctcagcgca tggcgcctga tcggtctaat ctcttatgct
      541 ctggaaccag cgggcttctg tggtttgctg ccgacgcccg agcgtcttcc tttacgggtg
      601 cgcgcatttg tcttggtccg ctgtccgcga acaggcaacc ctttgcgatg ccgaagccca
      661 cgaaatgttc cggtatccac caaacgcttg atattcatcg acacttcttt tcgcaagtca
      721 ccttccacgc ggtaatcccg ttcgatcacc tcgcgaatct tgacgatctt gtcttcgctg
      781 acatctttca cgcggatggc cccatcaacc ccagcctttt taagaatcgc ctgggccgca
      841 acgcgaccga ttccaaacac ataggtcaga ccaatttcga ttcgtttgtc ttttggcaaa
      901 tcaacgccag caatacgtgc cataattacc ccctagttcc ttttcttgct cgcctgatga
      961 gctggctcag ccacgttcag agattcgccc tgccgttgtt gtgcagacga ctccttccag
     1021 actccgcgca tgccgtgcca aggcaaccca ccgccgctca tccctgccgt tgtttatgac
     1081 gaggattggc gcataagacg cgaacgaccc cacgccggcg aaccacctta cattttgcac
     1141 aaatcggctt aactgacgac ttgaccttca tagcaattcc atttctccgc tacttgaaac
     1201 gataagtaat ccgtcccctc gtcaaatcat agggtgacaa ttgaatggtc accttgtcgc
     1261 caggaaggat ccgaatgaaa tgcatccgca tttttccgga aatatgagcc aaaatccggt
     1321 gcccgttttc caactctacg cgaaacatcg cattcgggag agtctcagcc accgtgccgg
     1381 tcacttctat gacatcttct tttggcacgt attggtacgc cttcccgtct tagtgtgaca
     1441 attgtgaaag aatcttggcc ggtccacttg gttgaatcgc gatggtgtgc tcaaaatgag
     1501 cagaccagcg gccatcttga gtgaccgccg tccaacgatc ttctaaaatg cgaacggcgc
     1561 tgcctcccat attgaccatg ggttcgatgg ccaataccat accgacttgg agccggggac
     1621 cttgcccagg tttgccataa tttggcactt gcggctcctc gtgaagctgc cttcctatac
     1681 catgaccgac aaattccgtc acaacggcaa aaccggcctg ctcgacatgg gtctgtatag
     1741 catgagacac atctgaaagg cgattcccga cgactgcttt cgcaattccc tggtacatcg
     1801 cttcttcggt cacctgtatc agacgtaacg cctcctcact tgccgatcca accggcacgg
     1861 taaccgcgga gtctccatag aagccggaaa cgatggcgcc caagtcaagc ccaataatgt
     1921 cgccttcctt gagtttgcgt ttcgatggaa tcccgtgaac gacctgttcg ttaatcgatg
     1981 cgcagagcgt tttgggataa ttccggtatc ctttgaaggc tgggatggcg ccacgaccac
     2041 ggatggcctc ttcagcgatc cgatccaagt cctcggtcgt aatgccaggt tcgactttcc
     2101 ccttcaacag cgcgaggact tctgccacca ctcgggaagc ctgcgccatt aatgtcacct
     2161 catccggcgt tttgaagacg atcattgagc gccgagcccg agcagcaacg cggaaagcct
     2221 cgtgtagaca gcggccattt cgccagagcc atccagatcg ctcaggaggt tctttgcttg
     2281 gtaatacgca atcagcgggg cggtttgctc gtcatacacc ttcaatcgag cctcaatcgt
     2341 ttcaggctta tcatcacttc gctggatcaa cgacgctccg caccgatcgc actggccctc
     2401 gattttcggt ggagcaaagg cgatgtgaaa cacgctttga catttagggc agctccgtct
     2461 tccactgaga cgtgtgacca catcatgccg ggaaacccgg aagttgataa cccgatcgag
     2521 cttcagattc cggccggtca agagagcctg taattcttca gcctgcggaa ccgttcgtgg
     2581 aaaaccgtcc agcacgaagc ccttctgaca cgaaggcgca gccaacgtct ctcgtaccat
     2641 cccgatgact acgctatccg gaactaactt tccggcatcc atgctcttct tggcttcaat
     2701 accgagtgcc gtttgatttc gaacggcttc ccgcagcaga tcaccggtag agatcttcgg
     2761 ccacccaaat tgagcggtaa cccgatctgc ctgagtgcct ttgccgacgc caggagcgcc
     2821 gagaaaaacg agtcgcatga ggtggtccta ggacgttctg cctcgaagtt ttcctttttg
     2881 aaggaatcca tcgtaattgc ggtttaggag atgtgactca atctgctgcg cagtatctaa
     2941 gccaactccg ataacgatca acagcgacgt accgccgaaa tagaacggca tattcagctt
     3001 atagataaga aattccggaa tcacgcacac gatcgccaga tagatggccc cggcaaacgt
     3061 gatcttcgtg agaacggaat aaatatagtc tgaggttcgc tgaccgggac gaataccggg
     3121 aacaaatccg ccatatttct tgaggttgtc ggccatatcc accggattca tgaccactgc
     3181 cgtatagaag aaacaaaaga atacgatcag gccgacgtac atgagggtgt aaaggagtga
     3241 tcccggcgcg agttgtgacc cgatagcctt aatccacggg gtttcaaaaa atccggcaat
     3301 cgtcgcagga aaggcaatga tggacgaggc aaaaatcggg ggaatgactc ccgcagtgtt
     3361 gatcttcaac ggaatatggg tgctttggcc cccgtagacc cttcttccaa tgacacgctt
     3421 tgcatactgc acagggactt ttcgtctccc gctctcaaga aagacgattg ctgcgaccac
     3481 accaaacatg acgaccgcca agacgatcag gagcgggaag cttaattgac cgaccttata
     3541 gagatcaaat gtttgagcca cggctgcggg aagtcgtgcc acaatccctg cgaaaataat
     3601 caaggaaata ccgttgccaa tgccgcgttc ggtgatctgc tcgccgagcc acatgaggaa
     3661 gccggtgccc gcacagaggg taatcaccgt catgaagcgg aatccccatc cgggactcag
     3721 gacgaacgca ccctggttca tctgttcaag accaacggca atcccgaacc cttggattaa
     3781 cgcaataaca atggttccga accgggtata ttggatgatc ttttttcgac cgcgttcccc
     3841 ttccttcgcc agcttcgaaa gatgcgggat aacgacagtg agtaactgaa gaataatcga
     3901 cgcgctgatg tagggcatga tgcccaacgc gaaaatcgtc aggcgtgaaa gagatccgcc
     3961 ggagaaaata tccaagaatc ccagaagcgc accaccttgc ttctgcagaa attcggataa
     4021 ggcttcaccg ttgatacctg gcgtggggat gtgggcaccg acgcgataga cgatcagcat
     4081 gcccagcgtg aacaatacgc gggtccgcag ctcaggtatc ttgaagatgt tttggaaact
     4141 ggtcagcaga cgctcaaaca cagggaatga cctcggccct ccccccggct gcctgaatct
     4201 ttgcctccgc tgacttgctg aacttgtgag cctggaccac gagaggtttt gtcaactctc
     4261 cctgggcgag gatcttaatc agctctcctt tgcgtcgaac aaggccttca ttcatcaaga
     4321 gttggggcgt aat
//
LOCUS       DAOQEW010000274         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8776_length_4332_cov_7.849427, whole genome shotgun sequence.
ACCESSION   DAOQEW010000274 DAOQEW010000000
VERSION     DAOQEW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8776_length_4332_cov_7.849427"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..751)
                     /locus_tag="PLS35_18380"
     CDS             complement(104..751)
                     /locus_tag="PLS35_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20694.1"
                     /translation="MSILIVDDSPDQQLLLKTILNKAGQHDLLIADSATSAYDQLGIQ
                     QDPSQEGPHAVDLILMDFLMPGIDGVAATRHIKSLDTLRDIPVIVVTAKTALGDLQAA
                     FAAGAMDYITKPVNSVELLARVNSALMLKKEMDCRKNRELELRRSNEDLQRALREVKV
                     LKGLVPICASCKKIRNDQGFWQQLEEYIQQHSEAEFSHGLCTPCIKKHYPGVYPD"
     gene            complement(909..1358)
                     /locus_tag="PLS35_18385"
     CDS             complement(909..1358)
                     /locus_tag="PLS35_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNI20695.1"
                     /translation="MSLHDRLSEDLKSAMKSRDQLRMDVIRMIKAAVQYKEVELKQDL
                     DDAGMSRIMTTLIKQRREAAEQFEKGNRQDLAAKERQEISIIEGYLPAAVSSDELANI
                     VAQVIKESGASSLKDMGQVMKAVMARLAGQSVDGKVVSDLVKTALQR"
     gene            1520..1726
                     /locus_tag="PLS35_18390"
     CDS             1520..1726
                     /locus_tag="PLS35_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20696.1"
                     /translation="MGQTNSSAAARVSGLVGIIVAAMVLVVGCASDKPKPMAQPSPEQ
                     VKGNADRAFDRLKQEEGERRPSGM"
     gene            complement(1751..3175)
                     /locus_tag="PLS35_18395"
     CDS             complement(1751..3175)
                     /locus_tag="PLS35_18395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNI20697.1"
                     /translation="MNHPTTAAPPSLSQPVLLPNETETAEHEAKASSRQYGIRDAACQ
                     AAAQGGGENYFSAFALLLHASPFHIAILSALPQLVGVVAQLASVKVLRYLRLPRRLLI
                     AGGRAQAFCWLPILSLPLIDPQHGPWLLIGCAMLYFGFGHVTAPVWNSLLVDVAEADS
                     RGAYFAQRARTTALTGFLALGLAGAVLTLAQRWDMSWIGFLVIFLGAAAARMGATRCQ
                     THVSRLMPVHQLDAPHGFRHFLANTASRDFRRFLLFSGLMHFAVLLSGPFFVIYLLRD
                     LHWSYLEYAGWMASSISAQFLTLGPWGRIGDRYGNKALLALTASAVPFLPMGYLLSEH
                     YLFLLTVNFFGGVIWAGLSLGLQNYVFDSLQPEERTRGVALANAMNAVGWGLGALTGS
                     WLATQMPTELSIGFWHLAPASNLPFLFCLSGVLRLAIAMSLLRTFAEPRRIASPPQGH
                     LVWELPVLKSLMTLMPWRNPRVAP"
     gene            3358..4017
                     /locus_tag="PLS35_18400"
     CDS             3358..4017
                     /locus_tag="PLS35_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20698.1"
                     /translation="MVAREGTRFSFPLALALCVTSILGWWQTGAEGRDLMVPKEQATT
                     EFEPTETPSLDVQQKGVPQSLFTWIKMARGYEVEWDTFGRKGWFTQDPRLKPIAPGTT
                     VFTPDTPAVYIVFEVAPLEDPAQFAAQVFAIGADGKTGDAVIVQDSLEVPGHERYGFL
                     ELKKPSSQWPQGKYVVKIYITSLGQQPFHAVNQVGTMRFAVTDQPSADVPPVVPGSPA
                     R"
     gene            4104..>4332
                     /locus_tag="PLS35_18405"
     CDS             4104..>4332
                     /locus_tag="PLS35_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20699.1"
                     /translation="MKETDLEKLTLLYERFCDVCLVEKEVWTEIYMPRTFKDGTAVRT
                     NIQDRYQVMIDDQGVEDALEANIPLGKAALAA"
BASE COUNT          920 a         1175 c         1357 g          880 t
ORIGIN      
        1 aggatggatt ggatcaacag acgatcgtct gtcgaatcat cgacgatcag gatacccatg
       61 atcagtgtct ccctgaaaat atgtcgtagg tcgatgctag cagctagtct gggtaaacac
      121 cagggtaatg ctttttgatg cagggggtgc agaggccgtg gctgaattcc gcctctgaat
      181 gctgttggat atattcttcc aattgttgcc agaagccttg gtcattgcgg attttcttgc
      241 aggaggcgca gatcggcacg aggcccttca gtaccttcac ttcgcgcaac gcccgttgca
      301 ggtcttcgtt gctccggcga agttccagtt cgcgattctt gcggcaatcc atttcctttt
      361 tgagcatgag tgcggaattg acccgggcaa gcaattccac gctgttcacc ggtttcgtaa
      421 tgtaatccat cgccccggcg gcaaaggcgg cctgcaagtc gcccagcgcg gttttggctg
      481 tgacgacgat caccggaata tcccgcaatg tgtccaagct cttgatgtgc cgcgtcgccg
      541 ccacgccgtc gatgccgggc atgagaaaat ccatgagaat caaatcaacg gcatggggcc
      601 cttcctgcga cggatcctgc tgaatgccga gctggtcgta ggctgaggtc gcagagtccg
      661 cgatgagcag gtcgtgctgg cctgctttat tgagaatggt cttgagcagc agttgctggt
      721 caggggagtc atcgacaatc aaaatgctca tgtcatcgcc tcgtcgggca gaggtggcgg
      781 agccggctcc tggttcccgg gagcatcacg aatggaggag aggcgtgagg gcatacccct
      841 tcgccgccgt acgtcggatt cagtatcggc gaaaagaccg aggaggttaa gtccggcagc
      901 gaggcggatc agcgttgaag agcggttttg accaggtcac tgacgacttt gccgtctacg
      961 gattgcccgg ccagacgagc catgacggct ttcatgacct gccccatgtc cttcagcgac
     1021 gatgcaccgg attctttgat gacctgtgcg acaatattcg ccagctcgtc ggaagagact
     1081 gcggcaggaa ggtagccctc gatgatgctg atctcttgcc gttctttcgc ggccaagtcc
     1141 tgtcgattgc ccttctcgaa ttgctcggcc gcttccctgc gctgtttgat gagagtggtc
     1201 atgatccggc tcatgccggc atcgtcgaga tcctgtttga gttcgacttc cttatattgc
     1261 acggcggctt tgatcatgcg aatgacgtcc atacgcagct ggtctctgga cttcatggcc
     1321 gatttcaggt cttcggataa gcgatcatgc agcgacatcg ggttcctttc agcagggaaa
     1381 tgcgtcgtcg cggagaataa cgcctttacc ggagccagtc aacgtggctg aggcgcgcac
     1441 gttttgtatt gagcgccggg aggtcctgtc ccataatgca atcggcggta gcgttggcac
     1501 ttaaccacgg aggctcagaa tggggcagac aaacagcagt gcagcggcgc gggtaagcgg
     1561 gctcgtcggt atcattgttg cggcgatggt gcttgtggtg ggctgtgcca gcgacaaacc
     1621 gaaaccgatg gctcagccgt cacctgagca ggtcaaaggg aatgcggatc gtgcgtttga
     1681 ccggttgaaa caggaagagg gagagcgccg gccatcgggg atgtaagcct ggcttcgggc
     1741 tgtgcgccct ctagggtgcc acgcgtgggt ttctccaggg catgagcgtc atgagggatt
     1801 tcaggacggg cagttcccag accagatgtc cttgtggcgg agaggcaatg cgccggggct
     1861 cagcaaacgt gcgcagcaga ctcatggcaa tagcaagtcg caagactccc gagaggcaaa
     1921 acaaaaacgg caggttcgac gccggcgcca gatgccagaa gccgatcgag agttcagtgg
     1981 gcatctgagt cgccagccaa ctgccggtca gtgcgccgag tccccatccc accgcattca
     2041 tggcattggc cagcgcgacc cctctggtgc gctcttccgg ttgtaatgaa tcgaagacat
     2101 aattctgcag gccgagcgac agtcccgccc agatcacgcc accgaaaaag ttgacggtca
     2161 acagaaacag gtagtgctcg ctcaacaggt agcccatcgg caggaacggc actgccgagg
     2221 ccgtcagggc cagtaaggct ttgttgccgt atcggtcacc gattcgaccc caggggccca
     2281 gcgtgagaaa ttgagccgag atactgctcg ccatccagcc ggcatattcg agataggacc
     2341 aatgaaggtc tcgcaggaga tagatcacaa aaaacggtcc cgacagcagt accgcgaaat
     2401 gcatcaaccc tgagaacaag aggaagcgac gaaaatcccg gctggcggtg ttggcaagaa
     2461 aatgccggaa gccgtgcggc gcatcgagct ggtggaccgg catcagccgt gacacgtgag
     2521 tctggcaacg tgtggctccc atacgcgcgg cagcggcgcc gaggaaaatg acaaggaagc
     2581 cgatccagct catgtcccat cgttgcgcca gcgtcaggac ggccccggcc agtcctaacg
     2641 ccagaaaacc cgtgagcgcg gtcgtgcgag cacgctgggc gaaataggcg ccgcgactgt
     2701 cggcttccgc gacgtcaacc aagagactat tccagaccgg ggcggtgaca tggccaaagc
     2761 cgaaatagag catggcacag ccgatcaaca gccatgggcc atgttgcggg tcaatcagcg
     2821 gcagcgacag gatcggaagc cagcagaagg cctgggcccg gcctccggcg atgagcaggc
     2881 gtcgtggcag acgaagatag cgaagcacct tcacggaggc gagttgtgcc actactccga
     2941 ccaattgcgg aagcgcggac agaatggcga tatgaaacgg agaagcgtgc aacaacaggg
     3001 cgaacgccga gaaatagttt tccccgccgc cttgggccgc ggcttggcag gccgcatccc
     3061 gaatgccgta ttgccggctg ctcgccttgg cctcgtgctc cgcggtttcc gtctcgttcg
     3121 gcaataggac tggctgagaa agtgagggcg gggcggcggt cgttgggtga ttcatgggac
     3181 tttgcgcggg aacaagtgcg cgatgcctac gaattttgcg ttgaatgaaa ggcattatat
     3241 cagagcgaac cgcgttgcct tctgttttga tcaaatcgcg caggccggtt gaccccctgt
     3301 tctcacagtg ggtaccataa ctgtatggat gcccgggttt cacaaggagc agcagacttg
     3361 gttgcccgtg aggggacgcg gttctccttc ccattggcgc tcgctctctg tgtcacctcg
     3421 atcctgggat ggtggcagac gggcgccgaa ggtcgcgatc tgatggttcc caaagagcag
     3481 gccaccacgg agttcgaacc caccgaaacg ccgtcactgg atgtgcaaca gaaaggcgtc
     3541 ccgcagtcac tcttcacctg gatcaagatg gcgcgcggct atgaagtcga gtgggatacg
     3601 tttggccgca aagggtggtt cacgcaggat ccacggctga aacccatcgc gccgggtacg
     3661 acggtcttca cgccggatac gccggctgtg tacatcgtgt tcgaagtcgc cccgctcgaa
     3721 gatccggccc agttcgccgc ccaggtgttt gccatcgggg ctgacgggaa aaccggcgat
     3781 gcggtgatcg tgcaggatag tctcgaggta ccgggccacg aacgatacgg ttttttggag
     3841 ttgaagaagc cttcgtcgca gtggccacag ggcaagtatg tggtcaagat ctacatcacg
     3901 tcgctcgggc agcaaccgtt ccatgccgtg aatcaagtgg gaacgatgcg atttgccgtg
     3961 accgaccagc cttccgcaga tgtgcccccc gtcgtgccgg gttctcccgc ccgttgaata
     4021 tcttcccctg cttagtctaa cgttcgtctg ccgcgtgggc tcgtgcggtc tttgaatgta
     4081 ttgtttatcg cgaggagcag gggatgaagg aaaccgatct ggagaaattg acgctgttgt
     4141 atgaacggtt ttgcgatgtc tgtttagtcg agaaggaagt gtggacggaa atatacatgc
     4201 cgcgcacgtt caaggacggc acggcggtgc ggaccaatat tcaggatcgt tatcaggtga
     4261 tgatcgacga tcagggcgtg gaggatgcgc tggaggccaa cattcctctt gggaaggccg
     4321 cattggctgc gg
//
LOCUS       DAOQEW010000275         4273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_8969_length_4273_cov_9.233997, whole genome shotgun sequence.
ACCESSION   DAOQEW010000275 DAOQEW010000000
VERSION     DAOQEW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_4273_cov_9.233997"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLS35_18410"
     CDS             complement(<1..248)
                     /locus_tag="PLS35_18410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20700.1"
                     /translation="MKTFSILCALSLVILGSSWAAGQTNRGNPRDGQAIYEKNCLRCH
                     GEKLDGNGPEGQYLIVRPANFQSVNSRAKTDWELLITIA"
     gene            complement(298..1155)
                     /locus_tag="PLS35_18415"
     CDS             complement(298..1155)
                     /locus_tag="PLS35_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20701.1"
                     /translation="MRVLIALDWSEQAFAAVREVSYLYDLQEVVLVHGIDLGMFQYPI
                     VAEVSNMQGYDEFRKAMEAAGQQLLDHTSTLLPSQGVSTTRVCEFAKPASLILDKARD
                     MRAELIVIGGRGRSRVGEFVLGSVSHRVALHADCTTLIVKEREGPVKRVTVAIEGHED
                     AERIKAWLLAHPFKNPVDLTIVSVVRPIPATDPFNLFPLQDWTGIAVRSAEDLVKNLA
                     AAVMNHRYSVGTQVAVGDPTDILTERARSSDLLIIGSHGRKRLERFLLGSISHALLHQ
                     VPCPVLLVR"
     gene            complement(1358..1909)
                     /locus_tag="PLS35_18420"
     CDS             complement(1358..1909)
                     /locus_tag="PLS35_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNI20702.1"
                     /translation="MGEQRRGWSLASGLLTGGILVLVAMLPIAKAEVQLLPGAAAELD
                     QATANAVLKTFQQAEEALRGRHVDGVMALYSEQYDYHGLHKKDMRKVWVDLLDEFQDL
                     ADVHHFSRMSKIGSGSKTIIEVTCTGSLTGLSKTGGLRVPIDSWYEEVHYMTWEDGQW
                     RIRGNVGDSPRFMPFGTSPHPLF"
     gene            complement(1975..2472)
                     /locus_tag="PLS35_18425"
     CDS             complement(1975..2472)
                     /locus_tag="PLS35_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HNI20703.1"
                     /translation="MMTNQPEGGHSARRDRFVEEYKHDSYKMPGKLKEPAVCKTCGAL
                     YHKGRWTWEIKPADAEQITCPACLRIQDKNPKGFVTLKGPYKAQHRDQVMGLIRNTEA
                     QEKKAYPLARIMTIEDREEGLVILTTDAHLPRRIGEALKHSHHGHLDIQYDQDEDFIR
                     ITWTG"
     gene            complement(2523..3176)
                     /locus_tag="PLS35_18430"
     CDS             complement(2523..3176)
                     /locus_tag="PLS35_18430"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNI20704.1"
                     /translation="MFKNREEAGELLAQELVAFRNAPDGILLALPRGGVTVAYQLSLA
                     LHLPLDVLITRKIGAPGNPEYALGAVSETGAVYWNQEALSGLSLSERDLAVAVHAQQI
                     EVSRRVALYRQGRPFPNLKDRTAIIVDDGLATGATFFASVATVRQARPRRIIGAIPVG
                     PRSTIQEARSLVDQLVVLRVPEPFYAVGNFYRDFEQVEDREVLQYLNLAEEAFLSKS"
     gene            complement(3231..3377)
                     /locus_tag="PLS35_18435"
     CDS             complement(3231..3377)
                     /locus_tag="PLS35_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20705.1"
                     /translation="MFKHPRYLDIPWEMHWHPEETSHHWWLILLVIIAALFLIGVGVT
                     SGLW"
     gene            complement(3393..3866)
                     /locus_tag="PLS35_18440"
     CDS             complement(3393..3866)
                     /locus_tag="PLS35_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNI20706.1"
                     /translation="MSGLTRWEPFRTQMNPWRELEEVQKRLSSMWGGAPQKEGSQKES
                     ISVAEWSPLVDITEDEKEYLIKAELPEMKKEHVKLTVHDNVLAISGERKYEKEEKDTK
                     YHRIERAYGSFMRSFTLPEDADGSKVSAEYKDGILNVHLPKSAQVKPKSIEVKVS"
     gene            3959..4120
                     /locus_tag="PLS35_18445"
     CDS             3959..4120
                     /locus_tag="PLS35_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20707.1"
                     /translation="MPGERMAFLEPRSPFAWQKNACDMVLPVGRYGTAAGTGNGANYL
                     IDFEATMLS"
BASE COUNT          817 a         1287 c         1197 g          972 t
ORIGIN      
        1 gcgatggtga tcaacaattc ccaatcggtc ttggcgcgcg agttcaccga ttgaaaattg
       61 gccgggcgga cgatgagata ctgaccctcc ggcccattgc cgtccaactt ttcgccatgg
      121 catcgcaggc aatttttttc gtagatggcc tgtccgtcgc gcggattgcc ccggttggtc
      181 tgtccggcgg cccaggaact tccgagaatc acaaggctga gggcacagag aatgctgaac
      241 gttttcatgc gctggctcct ttatggttca acgtttcgcc accggacggc catcacctta
      301 tcgtaccagt aacacgggac aaggaacttg gtgcaagagc gcatgggaaa tgctccccag
      361 gaggaaacgt tccagtcgct tcctcccgtg agacccgatg atcaacaggt cggacgaccg
      421 ggcgcgctct gtcaaaatat cagtcgggtc gcccacggcc acttgtgtgc cgaccgagta
      481 ccgatgattc atgacagcgg ctgcgagatt cttcaccaag tcctcagccg atcgtacggc
      541 gataccggtc cagtcctgga gaggaaacag gttgaacgga tccgtggccg ggattggccg
      601 cacgacgctc acgatcgtaa gatcgacagg atttttgaag ggatgcgcga gcagccaggc
      661 tttgatgcgc tcggcgtcct catggccttc gatcgccacc gtcactcgct tgaccgggcc
      721 ctcgcgttcc ttcacgatca aggtcgtaca atccgcgtgc agcgcgacac ggtgcgatac
      781 gctccccagg acgaattccc cgacgcggct tctgccacgc cctccgatca cgatcagctc
      841 cgcccgcatg tctcgcgcct tgtcgagaat cagcgacgcc ggtttggcga attcgcagac
      901 gcgcgtggtt gacacgcctt gggagggcag gagcgtggag gtatgatcca acaattgttg
      961 accagccgct tccatggcct tgcggaactc gtcgtagcct tgcatgttgc tcacttccgc
     1021 gacgatcgga tattgaaaca tgcccagatc gatgccatgt acgaggacga cctcctgcag
     1081 atcgtacagg tacgagactt ctcgaacggc tgcgaacgcc tgttccgacc aatcgagtgc
     1141 gatcaggacg cgcataggac tcctttcact agcccgcatg gttcatgagc actgctgctg
     1201 cactcgccga gtcactgctg cgacgggcct ttggctgctt ggctcgcccc tctggttctc
     1261 agcgtactcc aagcgtaccc ccagcctctt ccgtttccgc gcccctcctc gcaacgtgac
     1321 tcggcgatgt ctcgatgcat cgtcatgacc atgcgggcta aaacaacgga tgcggtgacg
     1381 tgccgaaggg catgaatcgc ggtgaatcgc cgacattgcc ccggatgcgc cactgtccgt
     1441 cttcccaagt catgtagtgc acttcctcat accagctgtc gatcggcacg cgcagccctc
     1501 cggttttgga gaggcctgtc aggctgcccg tgcaggtgac ctcaatgatg gtttttgatc
     1561 cagagccgat cttgctcatg cgggagaagt gatggacatc ggcgagatcc tgaaattcat
     1621 ccagcaaatc cacccagact ttgcgcatgt ctttcttgtg caatccgtga tagtcgtact
     1681 gctccgaata caacgccatc acgccatcga catggcgccc ccgaagcgcc tcttccgcct
     1741 gctgaaacgt tttcagcacc gcgttggccg tcgcctgatc gagctcagcg gctgcgccgg
     1801 gcagcagttg gacttctgcc ttggcgatcg gcagcatagc gaccagaacc aggattccac
     1861 cggtcagcaa cccgctagct agactccatc ctcgacgctg ttcgcccatg gttccctcgt
     1921 tgtgattcat ctgcccactt ctggcacggt gcataatccg ttcattcaag gcatctatcc
     1981 ggtccacgtg atccggataa agtcttcatc ctggtcatac tgaatgtcca agtgaccatg
     2041 gtgcgaatgt tttaaggctt caccgatgcg ccggggcagg tgggcgtcgg tggtgaggat
     2101 gacgagacct tcctcccgat cctcgatcgt cataatacga gccaggggat aggccttctt
     2161 ttcctgggcc tcggtattgc ggataagtcc catcacctga tcacgatgct gagccttgta
     2221 ggggcctttc aacgtcacga agcccttggg gttcttatcc tggatacgga ggcacgccgg
     2281 gcaggtgatc tgttcggcgt cggccggctt gatctcccag gtccatcgac ccttgtgata
     2341 caaggcaccg caagtcttgc agacggcagg ctctttcagc ttgcccggca tcttgtatga
     2401 atcgtgttta tattcttcga cgaaacgatc gcggcgcgcg ctgtgaccgc cttctggttg
     2461 gttggtcatc atgtccagtc cctttcttgg ctcgtctgcc aaccgttacg gcaactccag
     2521 acttacgatt tcgagagaaa tgcttcctcc gccaggttga ggtactgcaa cacctccctg
     2581 tcttcgacct gctcaaaatc ccggtagaaa ttcccgacgg cgtaaaatgg ctcgggaacc
     2641 cggagcacca ctaattgatc gacgagactg cgggcttcct ggatcgtgct acgcgggccg
     2701 accgggattg caccgatgat gcgccgcggg cgggcctggc gcacggttgc gacggaggca
     2761 aaaaaggtcg cgccggtggc gaggccgtcg tccacaataa tggcggtccg gtctttcaga
     2821 ttggggaagg gccgcccttg ccggtatagc gccacccgcc gggacacttc tatttgctgc
     2881 gcgtggacgg ctaccgcgag gtcgcgctct gaaagggaaa ggcctgagag ggcctcctga
     2941 ttccagtaga ccgcgcctgt ttcactgacc gcgccaagcg catattccgg attcccgggc
     3001 gcgccgatct tccgggtgat gagcacatca agcggaagat gcagggccag actcagttga
     3061 taggccaccg tgacgccccc gcgcggaagc gcgagcagga tgccgtcggg ggcgtttcga
     3121 aacgcgacaa gctcctgtgc cagcagttca ccggcctctt cacggttttt aaacatgacc
     3181 gtccgttcct ctatgcgctg gccgatcaac tggccacacc gtgagtcgag tcaccacaat
     3241 ccgctcgtca ccccgacgcc gatcaaaaac agggccgcga tgatcacgag cagtatcaac
     3301 caccagtggt gactggtttc ctccgggtgc caatgcattt cccacggaat atccagatag
     3361 cgaggatgct taaacatgag cgctcctttc gttcaggaga ccttcacctc gatggatttc
     3421 ggtttgacct gcgccgattt cgggagatgc acattgagga tcccgtcttt gtattctgcg
     3481 gagaccttgc tgccgtcggc atcttccggg agggtgaaac tccgcatgaa actcccgtac
     3541 gcgcgttcaa tacgatgata ttttgtgtcc ttttcttcct tctcgtactt gcgttcgccg
     3601 gagatggcca gcacattgtc gtgcaccgtc agcttgacgt gttctttctt catctccggc
     3661 agttccgcct taatcagata ctctttctcg tcttcggtga tgtccaccag aggtgaccat
     3721 tccgcgacgg agatggactc cttctggctt ccttccttct gcggcgctcc gccccacatg
     3781 gatgagaggc gtttctggac ttcttccaat tctctccagg gattcatctg tgtgcgaaat
     3841 ggttcccagc gagtcagtcc gctcatggcc gtactccttt atctattctg gtgatttctt
     3901 ggtttcgatg agtcccacag gtctccatgc ctcggagtat tgcaggcacg atgccagaat
     3961 gccgggtgaa cgtatggcct tcctggagcc gcgaagtcca ttcgcctggc agaaaaatgc
     4021 ctgcgacatg gtcctgccag tggggcgata cgggacagcg gcaggcaccg gcaatggtgc
     4081 caattacctc attgatttcg aggcgaccat gcttagctag atccttgaag ggaggtggag
     4141 ccaggataag gtcgacatcc ccgcttccgt gcagcgaaga gttggttcgg ggattgcgcc
     4201 tgtgccggtt tcgggatcag ttctgaaggg acgcgcttcc atctcggtgc cagtatgagg
     4261 cacggctccc atg
//
LOCUS       DAOQEW010000276         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9187_length_4208_cov_5.012280, whole genome shotgun sequence.
ACCESSION   DAOQEW010000276 DAOQEW010000000
VERSION     DAOQEW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_4208_cov_5.012280"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PLS35_18450"
     CDS             <1..1102
                     /locus_tag="PLS35_18450"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNI20708.1"
                     /translation="KSIATCLLPLVSICFAILCGEAVFRVYYWTKDGIPAREWHRFFL
                     GNFDGTMLDETLGWKATENYRANRAEKTKQGKSYVVTLSQNRYGFRQFGEIGSDKFKI
                     LILGDSFTHAREVSDSKTYYALLGRERPYEVFAYGVENYGTLQEYMILDRFFDLIKPD
                     LLVWQYCYNDFANNDPLMERRTMLHIDFRQRPYWSKGEIIRDMPSSILGKFRGLAYKY
                     SRFIVFLLSRMDRLYAAYLLDSVEAEILRSGAQHPDFRRAVRTTDELMATVRARVGAT
                     PVVAFNCKAVEPYNAALKNISGRHDIIFLDEIAEAVDFAARQGRDVFHADGAHWSEEG
                     HKLVGEMLVKRLEEFRVNRSLYRYREAAAPPN"
     gene            1130..2308
                     /locus_tag="PLS35_18455"
     CDS             1130..2308
                     /locus_tag="PLS35_18455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNI20709.1"
                     /translation="MSFTNSQAETPWVSGPFFHTYPIVSIIIPCRNERAFIAGCLESV
                     LANDFPKDRLEILVVDGLSDDGTREILDGFSRDHAFLSVLDNPKRVTPAALNLGIKHT
                     RGPIVMRMDAHARYDSHYISRCVDALGKYKVDNVGGIWKTLPRTETLAGWGVVKALSH
                     PFGVGNSHFRLPQGSEPKFVDTVPFFCVRRWVFQELGGFNESLVRGEDMEFSLRLNQP
                     GVFNERLTRGQDMEFSLRLRKAGGRTLLIPDIVSYYYARSDIKSFWCHNWINGVWAIL
                     PFAYSNVIPVSLRHLVPLFFVGSLVSTLVLTAWQGTFGWMFAGILGAYLLANVVASLH
                     VAWEARQPQYAVLMPIIFAMLHVGYGLGSLWGVVRLLTIREFWQKIFNVETNRAAPAR
                     "
     gene            2671..3300
                     /locus_tag="PLS35_18460"
     CDS             2671..3300
                     /locus_tag="PLS35_18460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNI20710.1"
                     /translation="MKRVFDVLCAVLGLMLLSPMLVVIALLITLGDGGSPFYRGLRVG
                     RNGRPFRILKFRTMRSDAERSGVTSTAKDDPRVTSVGAVLRRYKLDELPQLWNVLCGE
                     MSLVGPRPEVQRFVDLYTDEEKAILAVRPGLTDWASLWNIDEGAILEGSADPDRTYCE
                     LIRPKKIRLQLAYVRQAGFRTDLDILLRTVGAVLFRVKFREPRVLELGA"
     gene            3297..>4208
                     /locus_tag="PLS35_18465"
     CDS             3297..>4208
                     /locus_tag="PLS35_18465"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HNI20711.1"
                     /translation="MNFPDVHTGALSRDQLISLLRAMQRIRMAEERIGELVETREVRT
                     PCHLSIGQEAIPAGVCAALQQDDTLWGGHRSHGHYLAKGGDLRGMMAEIFGKATGCAR
                     GRGGSMHLVDPAQGIFGTVPLVAATIPLAVGAALSAKLRGTQQVAVAFFGDGATDEGH
                     FHESLNLAALYRLPVLFVCENNLYSTHLTLKERRVKDNIVESAALHGLPGVVVDGNDA
                     TAVYQTAHQAVQRARIGEGPTLLECRTYRWRGHVGPAADLEVGTDRRRELDEWQRRDP
                     IAQCRGLLKAHGLSSEVFDRLEAEVRRE"
BASE COUNT          878 a         1011 c         1259 g         1060 t
ORIGIN      
        1 taagtctatc gcgacgtgcc tccttcccct cgtctcaatt tgtttcgcca tattgtgtgg
       61 tgaagccgtg ttccgagttt actattggac gaaagatgga attcctgctc gcgaatggca
      121 ccgttttttt ctggggaatt tcgatggcac gatgttggac gagacactcg gctggaaggc
      181 tactgaaaac taccgcgcga acagggcgga aaaaactaag caaggcaagt cgtatgttgt
      241 gacgctgtcg cagaaccgct atgggtttcg ccaatttggt gagattgggt cggacaaatt
      301 taagatattg atacttggcg attcatttac gcatgcgcga gaagtttctg attcgaagac
      361 ttactatgct ttgcttggac gcgaacgtcc ttacgaagta tttgcgtatg gagtggaaaa
      421 ctatggcact ctgcaagagt atatgatcct ggatcggttt tttgatctca tcaagccgga
      481 tctgcttgtg tggcaatatt gctacaacga tttcgcgaat aatgatcccc tgatggagcg
      541 tcgcacgatg ctccatatag attttcgtca acgcccgtat tggagcaagg gggaaatcat
      601 acgtgacatg ccgagttcta ttctggggaa atttcggggg ctcgcttata agtattcacg
      661 gtttattgtt tttttgttga gccgaatgga tcggctctat gcggcctatc tgctcgatag
      721 cgtagaggct gaaatcttgc gatccggggc gcagcatcca gattttcgca gggcggttcg
      781 tacaacggat gaattgatgg caactgtgag agctcgtgtt ggagccacgc cagtagtggc
      841 cttcaattgc aaagcggtag agccctataa tgctgcactg aagaatattt caggccgaca
      901 tgacatcatc tttctcgatg agattgccga ggcggtcgac tttgcggcgc gtcaaggaag
      961 agatgtgttt catgctgacg gcgcccattg gagtgaagag ggccacaaac tagtcggtga
     1021 aatgttggtg aagcgcctag aggagtttcg ggtcaatcga tcgctttatc ggtatcggga
     1081 agcggcggct cctccaaact aggccatagg aactgattgg tgcacgcgca tgagtttcac
     1141 taactcacag gctgaaacgc catgggtctc tggtccgttt tttcacacat accctatcgt
     1201 ctcgattatc attccctgcc gaaatgagag ggcgttcatc gctggctgcc tcgaatccgt
     1261 gctcgcaaat gattttccca aagatcgctt ggaaatactc gtggttgacg gcctgagcga
     1321 tgatggcacg cgtgaaattc ttgacggctt ttcccgtgac catgcgttcc tttcggtgct
     1381 ggacaacccc aaacgtgtga ctcccgctgc cttgaacctc gggattaaac acacccgtgg
     1441 ccccattgtg atgcggatgg acgcccatgc tcgatatgac agccattaca tctcccgctg
     1501 cgtggatgct ctgggaaagt acaaagtcga caatgtggga ggaatttgga aaacgctgcc
     1561 tcgaacggag acattggcgg gatggggcgt cgttaaggct ctgtcccatc cattcggggt
     1621 tggcaattct cattttcggc tgccccaagg tagcgagccg aagtttgtcg atacggttcc
     1681 gttcttttgc gtgcgacgat gggtatttca ggaactcggt ggatttaatg aaagcctggt
     1741 ccggggcgaa gacatggagt ttagccttcg gttgaatcaa ccgggcgtgt tcaatgagcg
     1801 gctgacgcga gggcaagaca tggaattcag tcttcgtttg cggaaggccg gaggccgcac
     1861 ccttctgata cctgatatcg tcagttacta ttacgctcgg tcagacatca agtccttctg
     1921 gtgtcacaat tggatcaatg gcgtttgggc tattttgccc ttcgcgtata gcaatgtgat
     1981 tccggttagc ctacgtcacc tcgttccact gttttttgtc ggtagtttgg tatctacgct
     2041 cgtcttaacc gcgtggcagg ggacgttcgg atggatgttt gcgggcattc tcggcgccta
     2101 tctgctcgcg aatgtggtgg catcactcca tgtcgcctgg gaggcaaggc aaccacaata
     2161 cgcagtgctg atgccgataa tttttgcgat gttgcatgtc ggatacgggc tcggctccct
     2221 ctggggggtg gtcaggcttc ttacaatccg tgaattttgg cagaaaatat tcaatgtgga
     2281 gacaaaccgt gccgcccccg ccagataaaa cagacgtgga ggaagttttt ctggattcca
     2341 tcatgtttcg gaaatacagg tcgggcaatt cttgtacctt gccacgcagg tagcgcttcc
     2401 tttcaacatc ttccgttgat tattccgttc tcaaggcact tctggccccg aatgcagttc
     2461 tgctcagcgt gtggggtaca agtggcgatc atggatggct cggtttgcag tgggcaaatc
     2521 atgaggagag cgcgttgggc ctgaaaaaca ctcttgaagc ggtaccatct cagccgccat
     2581 actggcacgt tactgagcat gcgcgtgtag caggattctg gttgatcgat tcttgacact
     2641 gaagcagtgg gcggtagcgt cttttctatt atgaaacgag tatttgatgt tctttgtgcg
     2701 gtcctggggc taatgctgct ttctcccatg ctggtagtga ttgcactgtt gatcacattg
     2761 ggcgatgggg gctcaccgtt ttatcgtggg ttgcgtgtgg ggagaaacgg gcggccattc
     2821 cgtattttga aattccgcac gatgagatcg gatgccgaac ggtccggcgt gacctcaaca
     2881 gccaaggacg atcctcgggt gacgagcgtc ggggcggtgt taagacgata taaattggac
     2941 gaactcccgc aactctggaa tgtgctctgt ggcgaaatga gtcttgtcgg gccccggccg
     3001 gaagtgcagc gatttgtgga tttgtacaca gacgaagaaa aagccattct cgcagtgcgg
     3061 cccggtctca ccgattgggc gagtctctgg aacatcgatg agggagccat tcttgagggg
     3121 agtgctgatc ctgatcgaac ctattgtgag ctcatcagac cgaagaaaat ccgccttcaa
     3181 ctcgcgtatg tgcgccaggc cggcttccgg accgatctcg atattctgct ccggaccgtt
     3241 ggagcggttc tgttccgtgt gaaattccgc gaaccacgag tgctggagtt aggtgcatga
     3301 acttcccgga tgtgcataca ggtgcgttgt cgcgagacca actcatatcg ttgcttcgag
     3361 ccatgcagcg catccgcatg gctgaagagc gcattgggga actcgtcgaa actcgcgaag
     3421 tgcggacacc gtgccatctc tcgatcgggc aggaagcaat tcccgccggt gtctgcgcgg
     3481 cgttgcagca agacgacact ctgtggggcg ggcatcggtc ccatgggcac tatcttgcga
     3541 agggcggcga tctgcgcggc atgatggcgg agatctttgg caaagctaca ggttgcgcgc
     3601 gcggacgtgg aggttccatg catctggtgg atccggccca gggcatcttt ggcacggttc
     3661 cgttggtggc ggccaccatt ccgctggccg ttggtgccgc gctttcggcc aaattgcgag
     3721 gaacgcagca ggtggccgtg gcttttttcg gagatggtgc gaccgacgag gggcacttcc
     3781 acgagtcgct caatctggca gccttgtacc gtcttccagt cttgttcgta tgtgaaaata
     3841 atttgtactc cacgcatctg acgctgaaag agcgacgggt aaaggacaat atcgtggaga
     3901 gcgcagcgct gcatggattg cccggcgtgg tggtggacgg gaacgatgcg acggcggtct
     3961 atcagacggc gcatcaggcc gtgcagcgcg cgagaattgg cgagggcccc acgctgctgg
     4021 agtgtcgcac gtatcggtgg cgcggacatg tggggcctgc ggccgatctt gaggtcggta
     4081 cggatcgaag acgtgaactc gatgaatggc agcgccgcga tcctatcgcg cagtgccgtg
     4141 gcttgctgaa ggcacatggt ttgtcgtcag aagtctttga tcggctggag gccgaggtca
     4201 gaagggaa
//
LOCUS       DAOQEW010000277         4200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9214_length_4200_cov_8.901568, whole genome shotgun sequence.
ACCESSION   DAOQEW010000277 DAOQEW010000000
VERSION     DAOQEW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9214_length_4200_cov_8.901568"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLS35_18470"
     CDS             <1..210
                     /locus_tag="PLS35_18470"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HNI20712.1"
                     /translation="RDVRRFGAGRESGFVYHRARASLMPLAVLPWVTYLALPVSVHPL
                     LILLPSAALLGIAVDIASASFKKYL"
     gene            complement(262..>4200)
                     /locus_tag="PLS35_18475"
     CDS             complement(262..>4200)
                     /locus_tag="PLS35_18475"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNI20713.1"
                     /translation="SEPSGSVELARMFLVREFGRRPKRLNNLESMGLVAVRYPALENI
                     RQDPPAGALAAGFDQAAWLDFLKLCLDFYVRAGGSLAVSQSWRNWLGMPFPQSQLVAR
                     DEPHVGRNQRRWPRAKRSGLRSTPVRLLAHVLKADIATAHGEDRVDAVLQAAWDSLIS
                     AGLLQQAGDGRVLPLDSLAFAPMSQAWVCPVTRRFLDTTLRGVSPYLPQQATDATAHC
                     QRVELPLYDEPFGGVTDDLERIRRGRGWLTERADIAALRDQGLWSSLNDRVIELAPYF
                     TAAEHSAQQESRTLDRYEKAFKAGDLNLLSCSTTMEMGIDIGGISQVAMNNVPPHPAN
                     YLQRAGRAGRRREARSLAMTLCKSNPHDQAVFANSRWAFDSALPAPRVSLDSPIIVQR
                     HVHSLLLSRFLAEVLMGSGQEQTKLTCGLFFLGENSLADRFAAWCRGFSPGQSRGLTG
                     GLRQLLRHTVYDGNDLNGVMDAAAQAIDELARGWRTEWTNLEKEADELRRAGETSPAF
                     RAVALHKERLSGEYLLRELATRGNLPAYGFPTHIAPFDNLTRGQFLRARQQVADGRDD
                     NRYRRRELASRDLMTALREYAPGSEVVMDGLVYRSAGVTLNWHIPADQQDAREIQDIR
                     LAWRCNHCGASGSSHNLEAARHCDACGADIAPAHIREFLEPAGFAVDFYREPGNDVST
                     QHFVPVEAPWINARGDWFPLSNPALGRFRVSVKGHVFHQSRGIYGTGYALCLECGRAE
                     PVLPDGSLPDVFQKPHPKLRRTKEDGLFCRGSDDTWKVKNGITLGHESWTDVLEIQLK
                     TETGSWLNDATAAMTLAVALRDTLAELIGVQATELGCGIKEARPETGVHCQSIIIFDR
                     FAAGYASSAEQYLGSLFHDSRKRLECPADCDSACPHCVLDFDQRFAADSLNRRAALDV
                     LTDGWLKSLRLPDEYAYFGAESRPEYRSLPEAIWLAVSQQSASRVRFYTGGAQGAWDI
                     GPSPLRELTYHLAGKGVEVEIVVPDQMIATLDDADRFLLASLADHPKVAVRAIATPQQ
                     CGQGWLVAETLTSPASHWALDTEAALAFGLDWGKSEGPLITAGSATPSPLAGDALAAG
                     TIRPQRIDSGDREIEIHHELDGALQGFGRRFWQHISSQHPATRDLLADANGDLVSLTY
                     RDRYLFTPLSVALLAELVCGLRQLVGQERWAVRNLHVITTNRRSAGENTARNTIWSDW
                     PEMNVRHQVLEATFRYLGIDAFIQLADTATTGHGRLLEVEWSSGRKLTLRLDQGVSYW
                     RAAHSNSRQACYFNLSAGDADMRGKQLAELVVGIEGAHLPTQLFVKVRTP"
BASE COUNT          804 a         1299 c         1292 g          805 t
ORIGIN      
        1 cgggacgtgc gcaggttcgg cgccgggcgt gaatcaggct tcgtctatca ccgcgcgcga
       61 gccagcctga tgccgctggc cgtgctgccg tgggttacgt atctggcact gcccgtcagc
      121 gtccatccgc tgctgatcct ccttcccagc gcagcgctgc tcgggatcgc cgtggacatc
      181 gcgtcagcga gcttcaagaa atacctgtag tgcccgcgtt cctgtcttcc agactcgcct
      241 tgtggatggc atcttcgact atcatggagt gcggaccttg acgaaaagtt gggtcggaag
      301 atgcgcgcct tcaatgccaa ccaccaactc tgctagctgc ttgccccgca tatcagcgtc
      361 cccggcacta aggttaaagt agcaggcctg ccggctgttc gaatgggccg cgcgccagta
      421 tgagacgccc tgatcgagac gcaaggtcag tttcctacca gacgaccact ccacttccaa
      481 caaccgaccg tggccggttg tggccgtatc ggccagttgg atgaaagcat cgattcccag
      541 gtaacgaaag gttgcctcca aaacttggtg gcggacattc atctccggcc agtcggacca
      601 aatcgtattc cgggccgtgt tctctccggc gctacggcga ttcgttgtga tgacgtgaag
      661 attacggacc gcccatcgtt cctgccccac cagttggcgc aggccacaaa ccaattcggc
      721 aagcaacgca accgacaatg gtgtaaacaa ataacggtcg cgatacgtga gtgagacgag
      781 gtcgccattg gcgtcggcga ggagatctcg agtcgcagga tgctggctgg agatgtgctg
      841 ccagaatcgc cggccgaagc cttgaagcgc cccatccagt tcgtggtgga tctcgatctc
      901 gcggtcgcct gaatcaatcc gctgcggcct gatcgttcct gccgcaaggg catcgcccgc
      961 caggggcgac ggcgtggcac ttccagcggt gatcaacggc ccctcgctct tcccccaatc
     1021 gaggccgaat gccagggccg cttccgtgtc cagtgcccag tgcgatgccg ggctggtcag
     1081 agtctctgct accagccagc cctggccgca ctgctgtgga gtggcaatgg cgcggacagc
     1141 aaccttcggg tgatcggcga ggctggcgag aaggaatcga tcggcgtcgt ccagcgtggc
     1201 gatcatttgg tcgggcacca cgatttccac ctcgacgcct ttcccagcga ggtgatacgt
     1261 gagttcccgc aaaggggatg ggcctatatc ccacgctccc tgagcaccac cagtatagaa
     1321 ccgcactcgg ctagcgcttt gttggctgac agccagccaa atggcctccg gaaggctgcg
     1381 atactcaggt cgtgactcgg ctccaaagta ggcgtattca tcgggcaggc gaagactctt
     1441 cagccaccca tcggtgagca catcgagcgc tgcccgccga ttcaaactgt cggcagcgaa
     1501 gcgttgatcg aaatcgagta cgcagtgggg gcaagcgctg tcgcagtcgg ccgggcattc
     1561 cagacgcttg cgggaatcat ggaacaggct acccaggtac tgctcggcgc tcgatgcgta
     1621 ccctgcggcg aagcgatcga agattatgat cgactggcaa tgtacgccgg tttccggccg
     1681 tgcctccttg atgccgcaac cgagttccgt ggcctgcacg ccaatgagtt ccgccaaggt
     1741 atcgcgcaaa gccacggcga gggtcatcgc agccgttgcg tcgttcagcc agctaccggt
     1801 ttccgtcttg agctgaattt ccagaacatc ggtccagctc tcatggccca aggtaatgcc
     1861 gttcttaacc ttccaggtat cgtcgctgcc tctacagaag agaccatcct ccttggtcct
     1921 tcgcagtttg ggatgaggct tctgaaacac atcgggcagc gagccatcgg gaaggacagg
     1981 ttcggcgcgg ccgcattcca ggcataacgc atagcctgtt ccgtagatgc cccgtgattg
     2041 gtggaacacg tgtcctttga cagagacgcg gaatcggccc aaggcggggt tggagagagg
     2101 gaaccagtcg ccgcgcgcgt tgatccacgg agcttctacg ggaacgaaat gctgtgtgga
     2161 aacgtcattg ccgggctctc tgtagaaatc cacggcgaat ccggccggtt ccagaaactc
     2221 ccggatatgt gccggggcaa tatctgcgcc acaggcgtcg cagtgccggg ccgcttccag
     2281 gttgtggctg gaaccgctgg ccccgcaatg gttgcagcgc caagccaggc ggatgtcctg
     2341 aatctcgcgt gcatcctgct gatcggccgg aatatgccag ttaagcgtga cgccagcgga
     2401 tcgatagacc aacccatcca tgaccacttc cgagccgggg gcatactccc tcaatgctgt
     2461 catgaggtcg cggctggcca gttctcggcg gcgatagcgg ttgtcgtcac ggccgtctgc
     2521 gacctgttgc ctcgcgcgga gaaactgccc cctcgtgagg ttgtcgaaag gcgcgatatg
     2581 cgtgggaaat ccgtaagcgg gaagattgcc tcgggtggcc agttcccgta gcagatattc
     2641 gcccgaaagg cgttccttgt gcagcgccac cgctcggaaa gcgggagagg tttcacctgc
     2701 cctcctcagt tcgtcagcct ctttttccag gtttgtccac tccgttcgcc atcctcgagc
     2761 cagttcatcg atcgcctgag cggcggcgtc catgactccg ttcaagtcgt ttccgtcata
     2821 cacggtgtgg cgcaggagtt gccgtagccc tcctgttagc ccccgcgatt gacccggaga
     2881 gaaaccgcga caccacgctg caaagcggtc ggccaaggaa ttctcgccca ggaaaaagag
     2941 gccgcaggtg agcttggtct gctcctgacc actgcccatc agcacttccg ccaggaaacg
     3001 ggagagcaga agtgaatgga catgccgctg gacgatgatc gggctgtcca gggagacacg
     3061 gggcgcaggc aaagcactat cgaaagccca gcgactgttg gcgaacacgg cctggtcgtg
     3121 cgggttggat ttgcacaggg tcatagcaag cgaacgcgct tcccgccgcc gccctgcccg
     3181 ccccgctcgt tgcagatagt tggccgggtg cggcggcaca ttgttcatgg ccacttgact
     3241 gataccgccg atgtcgatgc ccatctccat tgtcgtggag caggacagaa ggttaaggtc
     3301 gccagccttg aacgccttct catagcgatc cagggttcgc gactcctgct gggcggagtg
     3361 ctcggcggcg gtgaaatacg gggcgagttc gatgaccctg tcgttcaagg aggaccataa
     3421 accctggtcg cgcagagcgg caatgtccgc gcgttcagtc agccaccctc gcccgcggcg
     3481 tatccgctcc aagtcatcgg tgaccccgcc aaacggttcg tcatataggg gaagctcgac
     3541 cctttggcaa tgcgccgtgg cgtctgtcgc ctgctgaggc agataaggac tgacgccgcg
     3601 caaggtggtg tcaagaaacc ggcgggtgac tgggcagacc caggcttggg acatgggcgc
     3661 gaatgccaga ctatcaagtg gcaggacccg gccatccccg gcttgttgta gcagccctgc
     3721 ggagatcagg gagtcccatg ccgcctggag gaccgcatcg acacgatctt cgccgtgggc
     3781 tgtggcgatg tccgctttga gcacgtgggc cagcagtcgt accggcgtgc tgcgcaagcc
     3841 actccgcttg gcgcgaggcc agcggcgctg attgcgacct acgtggggtt cgtctcgcgc
     3901 gaccaactgg ctctgcggaa acggcatccc cagccagttt cgccacgatt gtgaaacggc
     3961 cagcgaacca cctgcgcgga cgtagaaatc gaggcagagc ttgaggaaat ccagccaggc
     4021 agcctgatca aagcctgccg ccagggcacc cgcgggcggg tcttgccgga tgttctcgag
     4081 cgcgggatac cgcaccgcca ccagccccat gctttccagg ttgttgagcc gtttgggtcg
     4141 gcggccgaac tcccgaacca ggaacattcg cgccagttcc accgaaccgc tgggctcgct
//
LOCUS       DAOQEW010000278         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9250_length_4190_cov_6.607739, whole genome shotgun sequence.
ACCESSION   DAOQEW010000278 DAOQEW010000000
VERSION     DAOQEW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_4190_cov_6.607739"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..146
                     /locus_tag="PLS35_18480"
     CDS             15..146
                     /locus_tag="PLS35_18480"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20714.1"
                     /translation="MANLKPAKLMGIESQGMVLAAGDSEVRGLATFVEDVEPGTKVK"
     gene            143..1009
                     /locus_tag="PLS35_18485"
     CDS             143..1009
                     /locus_tag="PLS35_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20715.1"
                     /translation="MIRAFTIRLIGTGLLVLLSSMSAAFTPEPPESELESKAHYNDPL
                     PTTVLVRVVAHRSLVLGHEVGGARVTMTDVATGQLLASGLQQGEPGDQTQIMRTPHLM
                     DEPVYSSRPAAAFSATLELTRPTLVEITAEGPLAYPQALQRASTTVLLIPGHDLTNDG
                     IVLHLYGYLVQVEHPKPGEPLIAKEDVMLRASVRTLSGALVRPHSDWDSRKMHIYAEL
                     LIGDRIVERLQMFYAGEKSRFEAPFFVPLSKDAPDGITLRVVAADVATGNFGVSSAQF
                     PVLSERLRPKKN"
     gene            1127..2914
                     /gene="ftsH"
                     /locus_tag="PLS35_18490"
     CDS             1127..2914
                     /gene="ftsH"
                     /locus_tag="PLS35_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNI20716.1"
                     /translation="MDPKQRQFSIWYMFIALWVLILIQTFLPSLFNPTEIPYSEFKSA
                     VDANKVTEVTVSPQIIHGKMKEDKVFHTIRIEDPDLLRNLAQHQVKVTGMIESTLFRD
                     LLSWILPIVLFFGVWWFLLRRMGQSQGFMTVGQSKAKIYVENEVKVTFADVAGVDEAK
                     QELEEIIEFLKTPEKFRRLGGKIPKGILLVGPPGTGKTLLAKAVAGEAGVPFFSISGS
                     EFVEMFVGVGAARVRDLFEQAKGKAPCIIFLDELDALGKARGVGPMAHEEREQTLNQL
                     LVEMDGFDSRVGVILVAATNRPEILDPALLRAGRFDRQVLVDRPDKIGRLAILKVHAR
                     SITLANQADLETIAAMTPGFVGADLANLLNEAALLAVRRGKDTVSLSELQEAVERVIG
                     GLEKKNRVLNKMERARVAHHEVGHALMALSIPGGDAVHKISIIPRGIAALGYTMQLPT
                     EDRFLMTVSELKNRIAILLGGRAAEEVIYGEVSTGAQDDLRKATDIAKSMIKSYGMSE
                     KLGQVSLERDRQSVFLQTGPSQTPGDYSEHTSREIDCEVRLLIDEQYERARDLIKAQE
                     ATLRHAAHVLLEKETITGEELKALAAPPQ"
     gene            3038..3979
                     /locus_tag="PLS35_18495"
     CDS             3038..3979
                     /locus_tag="PLS35_18495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNI20717.1"
                     /translation="MLPHTYHELRSQLRIALAVNAVIVVAEFAGGFLTNSIGLIGDAG
                     HNLVDQGSLFLALYAHVLTARPATDSRTFGYHRAGIVAAFLNSFILLLTAGGITLVSI
                     ERLMAPVPVPGGWVMLIALISFAANLSIALLLQHGAKDDLNIRSAFWHMLSDAWVSLG
                     VVVSGGIIMVTGWSVLDPLVSLFVVLAIVRGAWPLFKESLEVLLESTPPGVSPALVAA
                     TIESIPGVKNVHDLHIWAVEPRLIMMTTHVQVDGGDQALTTDLLQTIRNRVTSEFKIK
                     HLTIQLETECCHPEAVHCDLSKLHDQHSHAEFLHTHH"
BASE COUNT          855 a         1386 c         1161 g          788 t
ORIGIN      
        1 aaaccatcgt catcgtggcg aacctcaaac cggcgaagct catgggcatc gaatcgcagg
       61 gcatggtcct cgcggcgggc gacagtgaag tgcgaggact cgccaccttc gtcgaagacg
      121 tggagcccgg caccaaagtg aaatgattcg cgccttcacc atccggctga tcggcaccgg
      181 ccttctcgtg ctgttgagca gcatgagcgc cgcgtttacg ccggagcccc ccgaatccga
      241 gctcgagagc aaagcccact acaacgatcc gctccctacc accgtgctcg tgcgcgtcgt
      301 cgcgcaccgc tctctggtgc tgggccacga agtcggcggc gcgcgcgtca ccatgacgga
      361 tgtggcgacc ggccagttgc tggcctcggg gctgcagcaa ggcgaaccgg gcgaccagac
      421 ccagatcatg cgcacgcccc atctcatgga tgagccggtc tacagcagcc gcccggccgc
      481 cgcgttcagc gccacacttg aattgacccg cccgaccctg gtggaaatca ccgccgaggg
      541 cccgctggcc tatccccagg cattgcagcg ggcgagcacc accgtgctgc tgattcccgg
      601 ccacgatctg acgaacgacg gcatcgtgct gcacctctac ggctacctgg tgcaagtcga
      661 acatcccaaa cccggcgaac ccctgatcgc caaggaagac gtgatgctgc gcgcgtcggt
      721 gcgcacactc tccggcgccc tggtccggcc acatagcgac tgggactccc ggaagatgca
      781 catctatgcc gaactgctga tcggggaccg catcgtcgag cgcctgcaga tgttctacgc
      841 cggagagaag agccgcttcg aagccccgtt ttttgttcca ctctccaagg acgcccccga
      901 tggcatcacg ttgcgggtcg tggcagccga tgttgcgacg ggcaacttcg gggtctcctc
      961 ggcgcaattt ccggtgctct ccgaacggct gagacccaag aagaattgac cccattgact
     1021 gaatcggtcg tttcatgatg tgctgacaag gcgctgcagt catgatctgc cgactgccgc
     1081 tcatcaccag tggagtccct gattacacga tcgtagattc ctcacgatgg atcccaaaca
     1141 acgacaattc tccatctggt acatgttcat cgccctctgg gtcctgatcc tgatccagac
     1201 ctttctcccg tccctgttca accccaccga aatcccgtac agcgaattca aatccgccgt
     1261 ggacgcgaac aaggtcaccg aggtgaccgt ctcgccgcaa atcattcacg gcaagatgaa
     1321 ggaagacaaa gtcttccata ccattcgcat cgaagacccg gacctgctgc gaaacctggc
     1381 ccaacaccag gtcaaggtca cgggcatgat cgagagcact ctgttccggg atctcttgtc
     1441 gtggattctt ccgatcgtgc tgttcttcgg ggtctggtgg tttctgctcc gccgcatggg
     1501 tcagagccag ggcttcatga cggtcgggca aagcaaagcc aaaatttatg tcgagaacga
     1561 agtgaaggtc accttcgccg atgtggcggg cgtcgatgag gcaaagcagg aactcgaaga
     1621 gatcatcgaa tttctcaaga cgccggaaaa attccggcgc ctcggcggga aaattcccaa
     1681 gggcattctg ctcgtcggcc ccccgggcac aggcaagacg ctactcgcga aggccgtggc
     1741 cggagaagcc ggtgtgccgt ttttttcgat cagcgggtct gaattcgtgg agatgttcgt
     1801 cggggtcggc gccgctcgtg tccgcgacct gttcgaacag gccaagggca aagcgccctg
     1861 catcatcttc ctcgatgagc tcgacgcgct ggggaaagcg cgcggggtcg gaccgatggc
     1921 ccatgaggaa cgggaacaga cgctcaacca gctgctggtc gaaatggatg gcttcgactc
     1981 ccgcgtcggc gtcatcctcg tggccgctac caaccgtcct gagattttgg accccgcact
     2041 gcttcgcgca ggccgcttcg atcgccaggt gctggtggac cggcccgaca agatcggccg
     2101 cctcgcgatt ctgaaagtcc acgcgcgcag catcaccctc gccaatcagg ccgacctcga
     2161 aactatcgcc gctatgacgc caggcttcgt cggcgccgac ctggccaatc tcctgaacga
     2221 agcagccctg ctggccgtac ggcgcggcaa ggacacggtg agtctgtccg aattacagga
     2281 agcggtcgag cgcgtcatcg gcgggctgga aaagaagaat cgggtgctga acaaaatgga
     2341 gcgcgcccgc gtggcgcatc atgaggtcgg ccatgcgctg atggccctgt ctatcccggg
     2401 cggcgatgcg gtccacaaaa tctccattat ccctcgaggt attgccgcgc tcggctacac
     2461 catgcagctc ccgaccgaag accgctttct catgacggtg tccgagctca agaaccgcat
     2521 tgccattctg ctgggcggac gcgccgcgga ggaagtcatc tacggcgagg tctccaccgg
     2581 cgctcaggac gatctgcgca aggccaccga tatcgccaag agcatgatca agtcatacgg
     2641 catgagcgaa aaacttgggc aggtcagcct ggagcgggac cggcaatccg tctttttgca
     2701 aaccggtccg tctcaaacgc ccggcgatta cagtgagcac acgtcgagag aaatcgattg
     2761 cgaagttcga ctcttgatcg acgagcaata cgaacgggca cgcgatctca tcaaagccca
     2821 ggaagccacg ctgcggcatg ctgctcacgt cttgcttgag aaagaaacaa ttaccggcga
     2881 ggaactcaaa gccctcgcgg caccccccca gtaacgccgc ctccttgcct cgcctctctc
     2941 ccttgagaat ccaagagatc tcgcgcactg gtccgcagtc acttgaagac cacacaacgg
     3001 cttgccctcc tccgcctctc gtgagacact gaagtcgatg cttccccaca cctaccatga
     3061 actccgctcg caactccgca tcgccctggc cgtcaatgct gtcatcgtcg tcgcggagtt
     3121 tgccggcggc tttctcacga acagcatcgg cctgatcgga gacgcggggc ataacctcgt
     3181 cgatcaaggc tctctcttcc tcgcgctcta tgcccatgtg ctgacggccc gtcccgccac
     3241 cgacagccgc accttcggct atcaccgggc aggaatcgtc gcggcgttcc tcaattcctt
     3301 catcctgctc ctcaccgccg gcggcatcac ccttgtcagt atcgaacgcc tcatggctcc
     3361 cgtccccgtt ccgggaggat gggtcatgct gatcgccctg atcagttttg ccgcgaacct
     3421 ttccatcgcg ctcttgctcc aacacggcgc gaaagatgac ctcaatatcc gcagcgcctt
     3481 ctggcacatg ctgagcgatg cctgggtgtc gctcggtgtc gtcgtgagcg gcggcattat
     3541 catggtcacc ggctggtccg tgctcgaccc cctggtgagt ctgttcgtgg tgctggccat
     3601 agtcagggga gcctggccgc tctttaaaga atcgcttgag gtcttactgg agtccacccc
     3661 gcccggcgta agcccggcgc tggtcgcagc aaccattgaa tccatccccg gggtaaagaa
     3721 cgtccacgac ctgcatatct gggcggtcga gccacgcctc atcatgatga ccacccatgt
     3781 acaggtcgac ggcggcgatc aggccttaac gaccgacctc ctccaaacaa tccgcaaccg
     3841 ggtcaccagt gaattcaaaa tcaaacatct gaccattcaa ctcgaaaccg aatgctgcca
     3901 ccccgaagcc gtccattgcg acctctccaa actccacgac cagcattcac acgcggaatt
     3961 tctgcacacg catcactagg cggatggtga aaaaatccgc cagcattgtt ctcgcatcgc
     4021 tcaaaggctc aacgtaccgc aagggtacgc ctcgcctcct cgctcgctgc ggccgcgctg
     4081 gcggactttt tgaccatccg ccgggaaacc tgcccaacca tctgcctgtg atcgcgagat
     4141 atctttgtgc caaagctgcg catgaaagcg ccacctagcc gccacaggac
//
LOCUS       DAOQEW010000279         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9301_length_4175_cov_7.037621, whole genome shotgun sequence.
ACCESSION   DAOQEW010000279 DAOQEW010000000
VERSION     DAOQEW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9301_length_4175_cov_7.037621"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLS35_18500"
     CDS             complement(<1..243)
                     /locus_tag="PLS35_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20718.1"
                     /translation="MTTRRSLSFVILLLVGGLLSVASLIVLSPPSIMAADPSNDVYFH
                     TPGTPSGPSAPTANDNHYPQVGSMDSRLLMWFIIQQH"
     gene            complement(240..1325)
                     /locus_tag="PLS35_18505"
     CDS             complement(240..1325)
                     /locus_tag="PLS35_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20719.1"
                     /translation="MLHRLALRVLPSLTLAVTEDSVRKRKRLVMFVPGLAAFAVYRAA
                     KQGFSLADPLTLLVLCGLVSMATALCAYRVGRGVPFRQLVTADGWQRIGWVLGWIGFV
                     YGIQLSLLVLALLWLVGYDYAKHPDGPAMMALIIPSTAVARDAFEIGYVRWLEACGRP
                     FITFPDGVALRNLLQRFDQALLTWVTAGVLGGVVLSALAAMAVSGDFAVLAQGGLVTL
                     AAGTVGLLAYFAGQGDQATLTSRLAATSWGELMKFWWWPGLAFAATYYLVLVGGCLYV
                     LKQPMNSIVLFAGVAALVGGVMPLYCYYLGYRREIESRQPGGVPSALLRCPFVMGILG
                     KSREAIVGSALSESAAVFEKSSQRVSS"
     gene            complement(1407..1901)
                     /locus_tag="PLS35_18510"
     CDS             complement(1407..1901)
                     /locus_tag="PLS35_18510"
                     /inference="COORDINATES: protein motif:HMM:NF019968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HNI20720.1"
                     /translation="MLVCGCGGWMHTEGIEERRSHGGDATWFVRTECRPCRWLVGIDM
                     PAGQVDGLVDRLMWSDDAKHRLDRVPPYAAPALRELVEGFARSRRHRVITYELIDQAL
                     TGDVVAWDADAEQRLANVPAPVRAMARIELERTAVDRGERSVSVALMEEVKARYFGMA
                     AQRA"
     gene            complement(1934..2650)
                     /locus_tag="PLS35_18515"
     CDS             complement(1934..2650)
                     /locus_tag="PLS35_18515"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNI20721.1"
                     /translation="MFEFLRQPGFFGTHATMGADLSQLMATLFTGLFIIGWIQAKQHR
                     GHPHHWLMLGGMITMVGFFTAYYLFRQLGVLAFEGKEGFGGSQALYDYVFIPVLTIHI
                     ILVIIGLVMAVYMIVLGFRSQQFIGGSRVLSPSLLQTSWKKVGLIFGVLLTVVIVLFG
                     TRVMSAGFSMRKLEVYLGFLSLVAIVFAVEMGIQRIWPDGGQRHRALGRFTMIVYCIL
                     FLTGTFTYAMLYILYPGKIG"
     gene            complement(2653..3648)
                     /locus_tag="PLS35_18520"
     CDS             complement(2653..3648)
                     /locus_tag="PLS35_18520"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNI20722.1"
                     /translation="MPRELSLAEVFQLGYYWETKILLTAVKLDVFSLLDGQGRSAAEV
                     AQKLGADSRALELLLNALVAIRLLTKTGAVYANTSVATTHLVKHGPQYIGHLLLLHDA
                     EWGNWGKLEEAIKTGRSPVTQHVFETDPALGANVLSVLHRIGQQSGPDLAKRLALGQA
                     KTMLDLGGGAGTNAIAFCQVYPSLSATVFDLATTLPVTERTIKDAGLEGRIALKAGDF
                     NRDALGGPYDVVLMSDILHYQDLAINAALVKKIHSHLNPGGRLIIKDRFLDASGTSPA
                     WTAAFAVHILVNTEQGACYRTAEAMQWMHDGGYVSVEEIERTAVVQGVKPSAGQS"
     gene            complement(3925..>4175)
                     /locus_tag="PLS35_18525"
     CDS             complement(3925..>4175)
                     /locus_tag="PLS35_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNI20723.1"
                     /translation="AEKAGYYRLQCDQHEFMQSFFLPVWNPYHAVVKDDGSFEIPGVP
                     AGKHKVVVWHPFVGKGKLNEFEIEVAEGGTANLKAEIK"
BASE COUNT         1052 a         1317 c         1063 g          743 t
ORIGIN      
        1 atgctgttga atgataaacc acatcaacaa ccggctatcc attgagccga cctgtggata
       61 gtggttgtca ttggccgtcg gggcagaagg gccgctggga gtgcctggcg tatggaaata
      121 cacatcgttc gacgggtccg ccgccatgat agacggagga ctcagcacga tgagactggc
      181 cactgacagc agcccgccaa ccaagagaag gatcacgaat gaaagcgaac gacgtgtagt
      241 catgacgaca ctctctggct gctcttctcg aagacagccg cagattcact caaggctgat
      301 cccacaatgg cttcgcgaga cttgcccaga atgcccatca cgaacgggca acgcagcaag
      361 gcgctcggca cgccaccggg ctgacgactt tcaatttctc gccgatatcc caagtagtag
      421 caatataacg gcatgacacc accgacaagg gcggccacgc cggcaaacaa tactatggag
      481 ttcatcggct gcttcaggac gtagaggcag cccccgacca ataccagata ataagtcgca
      541 gcaaaggcca atcctggcca ccaccagaat ttcatcaatt caccccagct cgtggcagcc
      601 agtctggagg tgagggtggc ttggtctcct tggccggcaa aatacgccaa cagaccaacc
      661 gtgcctgccg ccaaggtaac gagcccgcct tgggccaaca ccgcgaagtc gccactgaca
      721 gccatcgccg ccagcgcaga aaggaccaca cctccgagga cacctgctgt gacccaggtc
      781 aagagcgcct gatcgaatcg ttgcagcaaa ttccgtaacg ccacaccatc gggaaaggtg
      841 atgaacggtc gcccacaggc ctcaagccac cggacatagc cgatttcaaa tgcatcccgc
      901 gcgacggccg tactgggaat aatgagcgcc atcatagccg gcccatccgg gtgcttggca
      961 tagtcgtacc ccacaagcca caacagagcc aagaccagca gtgacaactg gattccatac
     1021 acaaacccga tccacccaag aacccatccg atacgctgcc atccatccgc tgtgacaagc
     1081 tgccgaaatg gcactcctcg cccgactcga taggcgcaca gcgccgtcgc catggacacc
     1141 agcccgcaaa gcaccagcaa tgtgagggga tcagcgagag agaagccctg cttggccgcc
     1201 cgataaaccg caaaggccgc taatccgggg acaaacataa cgagacgctt acgtttgcga
     1261 accgaatctt ctgtgacggc cagcgtcaaa ctcggaagca cgcgtagagc caaccgatgc
     1321 aacatagtgt catccacccg aatcaagaga gccagtcacg ggagggacac ctggtgacct
     1381 cctgcacacc gtctcaacac ctactactac gctcgctgtg cggccatgcc gaaatatcgc
     1441 gccttgacct cttccatcaa cgcgacactg accgaacgct cgccccgatc cacagccgtc
     1501 cgctcgagtt caatacgcgc catggcacgc acgggcgccg gcacgttggc aagccgctgc
     1561 tcggcatccg catcccaggc gacgacatcg ccggtcagtg cctgatcgat caactcgtac
     1621 gtgatgaccc ggtgccgcct ggatctggca aacccttcga ccaactcacg gagcgccggc
     1681 gccgcatagg ggggaacacg atccaggcga tgcttggcat cgtccgacca catcaagcga
     1741 tcaacgagtc catccacctg cccagcgggc atatcgattc cgaccagcca tcggcaggga
     1801 cgacattccg tgcggacgaa ccaggtcgcg tcgcccccat gcgaccgccg ttcctcgata
     1861 ccctcggtgt gcatccaccc gccgcatccg cacacaagca taagacgtcc agcctctcag
     1921 ccgttcccga tcgctaaccg attttgccag ggtacaaaat atacaacatg gcgtacgtaa
     1981 acgttcctgt caggaacaag atgcagtaaa caatcatcgt aaaccggccc agcgcccggt
     2041 gacgctgacc accatcaggc cagatccgct gaatgcccat ttccaccgca aacacaatcg
     2101 ccaccaggct gagaaatccg agatagacct ccagcttgcg catggaaaat ccggccgaca
     2161 tcacccgcgt cccgaagagg acgatcacca cggtcagcag aactccgaag atcagtccga
     2221 ctttcttcca ggaggtttgt agcaacgacg ggctcagtac acggcttcca ccgatgaatt
     2281 gctgagatcg aaaccccaac acaatcatgt agacggccat gaccagcccg ataatgacga
     2341 gaatgatgtg aatggtgagc accgggatga aaacatagtc gtacaacgct tgcgacccgc
     2401 cgaagccttc cttcccttcg aacgccaaca ctcctaattg ccgaaacaga taataggcgg
     2461 taaagaaccc caccatcgtg atcatgccgc ccagcatcaa ccaatgatgc ggatgtccgc
     2521 gatgttgctt cgcctgaatc cacccgatga tgaagaggcc ggtaaacagc gtcgccatca
     2581 attggctgag atcggccccc atcgtggcgt gggttccgaa gaaccccggc tgtcgaagga
     2641 attcaaacat cgtcaactct ggccggcact tggcttcaca ccctgcacca ccgccgtccg
     2701 ttcaatctcc tcaaccgaaa catagccgcc gtcatgcatc cattgcatgg cctcagccgt
     2761 gcgataacag gcgccctgtt ccgtattgac gagaatgtga acagcaaagg cagccgtcca
     2821 ggccgggctg gtgccggacg catcgaggaa ccgatccttg atgatcaggc gcccaccggg
     2881 attcagatgg ctgtgaatct tcttcaccaa cgccgcattt atcgcaaggt cttggtaatg
     2941 gagaatgtcc gacatcaaca cgacatcata agggccgccg agggcatcgc gattgaagtc
     3001 accggccttc aacgcaattc gcccctctag acctgcatct ttgatcgtcc gttcagtcac
     3061 ggggagagtc gtggcgagat cgaataccgt cgctgagagg ctcggataca cctgacaaaa
     3121 ggcgatggca ttggtgcccg cgccaccacc gagatccagc atagtcttcg cctgccccag
     3181 cgcgagtcgc ttcgccaggt ccggcccact ttgctggccg atccggtgca ggacagacag
     3241 gacattggcg ccaagcgccg gatccgtctc aaacacatgt tgcgtgaccg gggatcggcc
     3301 tgttttgata gcctcttcca acttgcccca attcccccat tccgcatcgt gaaggaggag
     3361 caaatggccg atatattgag gtccgtgctt aacgagatgg gtcgtggcca cagaagtatt
     3421 agcatacacg gctccggttt tcgtcaacaa ccggatggcc accaaggcgt tgagcagcaa
     3481 ctccagggca cgagaatcag caccgagttt ttgagccact tctgcggcgg aacgcccctg
     3541 accgtcgagc agggaaaata catctaactt tacggcagtt aaaaggattt tggtctccca
     3601 gtaatagccc aactggaaga cttcggcgag agacaattcg cgaggcacag atcccccact
     3661 tagtgattga ggcaattgaa gttggtcatc ttaccgagtt cagaaaggga aacgcaaggc
     3721 gaggagcggg caggaacgag tctgacaatc gaccaatctt gctggttgca tcgcaacaaa
     3781 ctgagaacga cgcatcgtcg aaattgcgct gactatctct actttactga ggagtcgtac
     3841 gattcaagac aaacaaaaag ggcagtggtt caattcgaac cactgccctt tcctcagcag
     3901 gttatcccgc taaagtaaaa ccgactactt gatttcagcc ttcaagttcg ccgtgccgcc
     3961 ttcggccact tcgatctcga attcattcag cttgcccttg ccaacgaacg gatgccaaac
     4021 aaccactttg tgcttgccgg caggcacgcc cgggatctcg aacgagccat catccttcac
     4081 caccgcgtgg tacggattcc acaccggcaa gaagaaggac tgcatgaact catgctggtc
     4141 gcactgcaac cgatagtagc cagccttttc ggcgc
//
LOCUS       DAOQEW010000280         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9314_length_4172_cov_8.188730, whole genome shotgun sequence.
ACCESSION   DAOQEW010000280 DAOQEW010000000
VERSION     DAOQEW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9314_length_4172_cov_8.188730"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLS35_18530"
     CDS             complement(<1..385)
                     /locus_tag="PLS35_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNI20724.1"
                     /translation="MRGIAMWAASAAVLAMSGTLYALDVADVVREWTPEGKKLAAERV
                     KLPAHDEMIRIPAGGFLMGSDRKVDKNSYLAEFPQRKVYLDAYDIDKYEVTTVQFLKY
                     VLAHNLDPLIDWQYDGGNFQDTMVSH"
     gene            complement(420..1079)
                     /locus_tag="PLS35_18535"
     CDS             complement(420..1079)
                     /locus_tag="PLS35_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20725.1"
                     /translation="MTMCIKQTGKFDRVIGKIRRMCLWKLAGSALALTATIGIMDAAA
                     LEFTADQITKINGRTQKSNIFYRDNMWRIEHHTMGPVNISIVRKDKQVVWLLLSRMKH
                     FKTVPYKAEQDLKVSEHLEGEISREEIGTETREGHPTTLYEVTVKEGERTEVYYQWLA
                     TDIHFPMKLAKKDGSWIVEYQHVKFRPLIDYLFQLPLNFEPLEEFDHQPAAAEPNHQP
                     M"
     gene            complement(1362..1973)
                     /locus_tag="PLS35_18540"
     CDS             complement(1362..1973)
                     /locus_tag="PLS35_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNI20726.1"
                     /translation="MTKRTGRALAMWCLGTGMLLSATFAQAEMARYDVDLDHSIVEFK
                     VAHMVVSKTTGHFKDYTGFIEMDPDAGTVKALEATIKTASVTTNHEKRDAHLRNPDFF
                     DAEKYPTITYTMKSYKKAGDGYVAVGDITLHGVTKEITLTGNFNGVAKDPWGNTRAGF
                     TAEGKLNRKDFGMVWNKALDSGGLVVGDDVFIRLDIECIKAKS"
     gene            complement(1976..3001)
                     /locus_tag="PLS35_18545"
     CDS             complement(1976..3001)
                     /locus_tag="PLS35_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNI20727.1"
                     /translation="MQHQLHSTCAGLILGLGVMFCVSVPLSATAGPSANQAPAAPKRW
                     AQFDLVSTEPDGSVQAGKDVVLSITLGGVPAGTESVMAMIESNGFQSQTVSLSEEPTA
                     SVYQGTVILEPNSTLKSRTTVHPKAVRVRVSFARAKVTGLEEFLKRDVYVTIGEPPAN
                     ETETEVQPGPVAESPEADAAAVQKATEQAMAVNATIAEEDLLPLPPPGESKAYWKQVS
                     DLISRNWSRQIRSIRRAPSSETVRVQFKMYASGIAQVIQLEKSSGARDVNDAGLQTII
                     HAQPFPPIPPDLGSDVVDVHVRMRTGAKVATRDVEMTVDKKPSKTAPPSAPTPKEGAA
                     ASGSTKE"
     gene            3154..>4172
                     /gene="tdh"
                     /locus_tag="PLS35_18550"
     CDS             3154..>4172
                     /gene="tdh"
                     /locus_tag="PLS35_18550"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNI20728.1"
                     /translation="MRALVKTAAQPGLTYTERPDPSPGPNDAVIRVKATSLCGTDAHI
                     YNWDAWAHSRIHPPRIIGHEMCGEVVAVGSDVSLVTVGDYVAAESHLTCGACFQCRTG
                     QAHVCKNYRILGVDLDGSFADYIVLPETVLWKTSPDIPPELASLQEPLGNAVDAALVE
                     DLTGHTVLITGCGPTGLFAAAVARTAGAATIIATDVSDYRLGLAKQVGVDHVLNAKTD
                     GPDAIAAAILDITKGEGVDASLEMSGHPTALHHAFRSVKNGGRVTLFGIPTGPVTCDL
                     ANEVIFKGIRVHGITGRRLFSTWYRLAGLFKAGLNIRPVLTHTFPLKDFAQGFELIKS
                     GQCGKV"
BASE COUNT          803 a         1293 c         1115 g          961 t
ORIGIN      
        1 gatggctgac catggtgtct tgaaaattcc cgccgtcgta ctgccaatcg atcagcgggt
       61 cgagattgtg ggccagcaca tatttgagaa actgcacggt cgtcacttcg tatttgtcga
      121 tatcgtaggc atccaaatag actttccgtt gcggaaattc ggccaggtag gaattcttgt
      181 cgaccttgcg gtcgcttccc ataagaaacc cgccggctgg aatacgaatc atctcatcgt
      241 gtgccggcaa cttcacgcgt tccgctgcca gctttttccc ttccggcgtc cattccctga
      301 cgacatcggc gacatcgagc gcataaagag tcccgctcat tgccaacacg gccgcgctcg
      361 cggcccacat ggctattcct cgcatacgca cgattttgct cctttatccg ttgatcatcc
      421 tacatcggct ggtgattcgg ctccgcagca gccggctggt gatcaaattc ttccaatggt
      481 tcgaagttga gcggtaactg aaacagataa tcgattaatg gccggaattt gacgtgctga
      541 tactcgacga tccagctccc gtcttttttg gccagcttca tggggaagtg gatatcggtg
      601 gcaagccact ggtaatacac ttctgtccgt tcgccttcct tgaccgtcac ttcatacagc
      661 gtcgtcggat gcccttcgcg cgtttccgtt ccaatttctt cacgcgagat ttcaccttcc
      721 agatgctcgg acaccttgag atcctgctcc gccttatagg gcacggtttt gaaatgcttc
      781 atacgcgaca gcagcagcca gacgacctgc ttgtctttac gcacgatgct gatgttgacc
      841 ggccccatcg tgtgatgctc gatccgccac atgttgtcgc gatagaagat attggatttc
      901 tgggtccggc cattgatctt ggtgatctga tcggccgtaa actctaacgc cgcggcatcc
      961 atgatgccga tcgttgctgt cagcgccagc gctgaccctg ccagtttcca caagcacatg
     1021 cggcgaatct taccgatgac gcgatcgaat tttccagtct gcttgataca catcgtcata
     1081 ccctctgcgt ccccttcacg cactccgtcc tcctccgtcc tcccccgtcc gtccaagccc
     1141 tgcgcgttga aaccggcaca ccaagaatcg acagcgaaca agatgtccag gacatcctcg
     1201 ctccactatg accagctgct gtgtctgctg aaaccggcgt cacgagatgg gaacgggttg
     1261 catccccaga aagggatact ccgccagatg cgcggttgct ggctcagcct gcccggcaca
     1321 gaaccgggca ggcgcgtggc ccctgagaga ggccgtgtgg cttacgattt cgccttaata
     1381 cattcgatgt ccaatctgat aaaaacgtcg tctccgacga ccaggccgcc gctatcgagc
     1441 gccttgttcc acaccatccc gaaatccttc cgattcagtt tgccttcggc cgtaaacccg
     1501 gcgcgcgtgt tcccccacgg atccttcgcg acaccattga aattccccgt cagtgtgatc
     1561 tccttggtca caccgtgcag ggtgatatcg ccgaccgcca catatccgtc gccggctttt
     1621 ttataacttt tcatggtgta ggtgatagtc gggtactttt cagcatcaaa gaagtcgggg
     1681 ttgcgtaaat gcgcgtcgcg tttttcgtga ttggtcgtca ccgacgcggt cttgatcgtg
     1741 gcttcaagcg ccttgaccgt tccggcatcg ggatccattt cgatgaagcc ggtgtagtcc
     1801 ttgaaatgcc cggtcgtctt cgacaccacc atgtgggcaa ccttgaactc cacgattgag
     1861 tggtcgagat ccacgtcata ccgtgccatt tctgcttgcg cgaaggttgc actcagcagc
     1921 attcccgttc ccagacacca catggctagc gcacgaccgg tccgtttcgt catagttact
     1981 cctttgtaga accgcttgcc gcggctcctt ctttgggtgt tggggcggag ggtggagcag
     2041 ttttggacgg ctttttatca accgtcattt ccacatcgcg cgtggcgact ttcgcccccg
     2101 ttcgcatacg cacatgcaca tcgacgacat cgctgccgag atcgggggga atcggtggaa
     2161 acggctgggc atggatgatg gtctgaagcc cggcatcgtt cacgtcacga gccccggaac
     2221 ttttctccaa ttgaatcact tgggcaatcc cgctcgcata cattttgaac tgcacacgaa
     2281 cggtttcact gctgggcgcg cgacgaatag aacggatttg ccggctccaa ttccggctga
     2341 tcagatcact cacctgcttc caataggctt tcgattcgcc gggaggagga agcggcagca
     2401 gatcttcttc ggcgatcgtc gcgttaacgg ccatcgcctg ctccgtcgcc ttttgcacgg
     2461 ccgccgcatc ggcttccgga ctttcggcca ccggtccagg ttggacctca gtctcggttt
     2521 cgttggcggg cggctctccg atcgtcacat agacgtctcg tttcagaaac tcttccagcc
     2581 cggtgacctt cgcgcgggcg aacgacaccc ggactcgcac ggctttcgga tgcaccgtgg
     2641 tgcgactctt caacgtggaa ttgggttcca gaatcaccgt gccttgatac accgaagccg
     2701 tcggctcctc actcaaggac accgtctggc tttgaaatcc gttggactcg atcatggcca
     2761 tgacggattc cgttccggcc ggtacgccac ccaacgtgat gctcaacacc acatctttgc
     2821 cggcctgcac cgacccatcc ggttcggtac tgaccagatc aaattgggcc cagcgtttcg
     2881 gggcggccgg agcctgatta gcggacggac cggccgtggc tgaaagtggc acacttacgc
     2941 agaacatcac tcccagtcca aggatgagac ccgcgcaggt ggaatgtagc tggtgctgca
     3001 tgctcgcatc ctacccaggc gccctaccca gttcaacccg ttttttgggc gccctcggct
     3061 acgagctgat ccggggcatg atccaccggc gggagatccc gacgggtttc tgcttcggct
     3121 aggcatttct gatggtcttg ctataatgag cccatgcgtg cgcttgtgaa aactgccgcc
     3181 cagccgggcc tgacctacac cgaacgtccc gatcccagcc cagggcctaa cgacgcggtg
     3241 atccgggtca aagcaacatc gctctgcgga accgatgccc acatctacaa ctgggatgcc
     3301 tgggcccaca gtcggatcca tcctccacga atcatcggcc acgaaatgtg cggcgaggtc
     3361 gtcgcagtcg ggtcggatgt gagcctggtg acggtgggtg actatgtcgc ggccgaatca
     3421 cacttgacct gtggcgcctg ctttcaatgc cgtacgggac aagcgcatgt ctgcaagaat
     3481 taccgcatcc tcggcgtcga tctggatggg tcgtttgccg actacatcgt cctgccggag
     3541 actgtcctct ggaaaacatc gccggacatt ccccccgaac tggcctcttt gcaggaaccc
     3601 ttgggcaatg cggtcgatgc ggcgctggtc gaagatctga ccggacacac cgtcttgatt
     3661 actggttgcg ggccgaccgg cctctttgcc gccgccgtcg cccgcacagc aggcgccgcc
     3721 accattatcg ccaccgatgt cagtgactac cgtctcggat tagcgaaaca ggtaggggtc
     3781 gaccacgtgc tgaacgcgaa aactgacgga ccggacgcca ttgccgcggc tattctcgac
     3841 atcaccaagg gtgaaggcgt cgatgcctcg ctggagatgt cggggcaccc caccgcgttg
     3901 caccatgcct ttcgttccgt aaaaaacggc ggccgcgtca cgctgttcgg cattcccacc
     3961 ggtcccgtga cctgcgatct cgccaatgaa gtgatattca aaggcattcg tgtccacggc
     4021 atcaccggcc ggcggctgtt cagcacctgg tatcggctgg cgggactgtt caaggccggg
     4081 ctcaacatcc ggccggtttt gacccatacc tttccgctga aagactttgc ccaaggattt
     4141 gaattgatca agtccggcca atgcggcaaa gt
//
LOCUS       DAOQEW010000281         4160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9356_length_4160_cov_9.148843, whole genome shotgun sequence.
ACCESSION   DAOQEW010000281 DAOQEW010000000
VERSION     DAOQEW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_4160_cov_9.148843"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..727)
                     /locus_tag="PLS35_18555"
     CDS             complement(17..727)
                     /locus_tag="PLS35_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNI20729.1"
                     /translation="MNTFTAEVSRIHDLTHDVRAIELRLLEPASITFKAGQFVSFEVP
                     KEGHPRSVTRPYSIASPPEERERILLVLNLVQGGPGSNYLFSLREGNRTSFKGPAGAF
                     YLRDDGTKDLCFVATGTGIAPVRSMILAQLQRRPDRPVTLFWGLRSQRDLYWQDEWAA
                     LAAIHPHLTVVTTLSRPEPGWQGASGRVTALVEERITSVRNLAVYLCGGNGMIKDVTA
                     RINAKGLCPIYREKYYDE"
     gene            complement(776..1489)
                     /locus_tag="PLS35_18560"
     CDS             complement(776..1489)
                     /locus_tag="PLS35_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNI20730.1"
                     /translation="MKILLLLLGGLKFGKIALTGGTMLLSMVAYSFFFGWWYAVGFVL
                     LILFHELGHYAAAKQRNLNVGAPTFIPFVGAWIQLKEQPMDVETEAYVGIAGPVAGTV
                     AAIACYYAAEYTQSQLLLALAYAGFMINLFNLIPLSPLDGGRITAIISPKVWWVGVPI
                     LIGLFIMNHSPMLLLIAIMAIPHVMSTFRGGPHGLPERYYDVPLSTRVNYGLYYLGLA
                     AFLGIMTYETHLLLPSGRG"
     gene            complement(1486..2385)
                     /locus_tag="PLS35_18565"
     CDS             complement(1486..2385)
                     /locus_tag="PLS35_18565"
                     /inference="COORDINATES: protein motif:HMM:NF021454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HNI20731.1"
                     /translation="MTWCHWRLMTAFLCWMVAGVALPALAEEPKPSGLHWLEGPTEAK
                     LGDQALLKLPKGYQFLGAQETQALLKRMGNFPSGSELGLITATGEGEQWFMVVRYIDA
                     GYVKDDDAANWDADALMTSIKEGTEEDNKNRQAQGFPPLIIRGWEEKPHYDKAANKVV
                     WAISAQEPESPVGVNYNTLALGRQGYLSMNMVGSLEQLPVLKPHVSQLLANVEFVEGK
                     RYTDFNSTTDKVAAVGLTALIAGAAVKSGLLAKLWAFIIPLLVAGKKLFMLLVIALGG
                     LAAKYLKKKPQAEQPGGGGGVSS"
     gene            complement(2565..2966)
                     /locus_tag="PLS35_18570"
     CDS             complement(2565..2966)
                     /locus_tag="PLS35_18570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNI20732.1"
                     /translation="MKRSKALVLLDQREGEGPEAQRGDRVLYNCRLFLHCGDEVPLNE
                     RQAATLPGHMLRVDGGVTLVDHQVELGRRQVIPGIEQALLGMRVGGYRKVRISPHLAY
                     RDKGLPSLIPANALLVAEVWVRKIVKPEARE"
     gene            complement(2972..4084)
                     /locus_tag="PLS35_18575"
     CDS             complement(2972..4084)
                     /locus_tag="PLS35_18575"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNI20733.1"
                     /translation="MVSLQRFQSVLGKPYLPPLFFFSGVTYDTLVLTRIDRLRDNLLL
                     LAYLTLLGFLIVLTGRLGTQEATVDDLPPDAPFFMKWIVQAKPYAPMAIQFLLGGLFS
                     AYAIFYSKSATFAGTAVFFCVLVAFLVANEFLRSRLSNIQLLVSLYALVCFAFFTFFL
                     PVMTGWMNAVIFLAGAMVTVLIVLRVVQLIYWKNADRTTREAMWAGAPAVGLIALLVG
                     FYFMNWIPPVPLSLKFGGMYHEVKRSSDRYELSFEKSWYEVWKTSDNVIPADDPVYCF
                     TAVFAPVALKTTIYHHWYYRPDAGQPFADADRIPLKISGGREGGYRAYTFKEGLDPGD
                     WRVDVEAEDGRVIGRVAVKVLNKTTVGPLSLTTVVY"
BASE COUNT          928 a         1296 c         1135 g          801 t
ORIGIN      
        1 cctctttcat cggccgtcac tcgtcgtagt atttttcgcg atagatcgga cacaatccct
       61 tggcgttgat ccgtgccgtc acatccttga tcatcccatt accgccgcaa aggtagaccg
      121 cgagattcct gaccgaggtg atccgttcct ccaccaacgc cgtgacgcgc ccactcgccc
      181 cctgccaccc cggctccggt ctggacagtg tggtaacgac ggtcaagtgg ggatggatcg
      241 cggccagcgc agcccattca tcctgccaat acagatcccg ctggcttcga agcccccaga
      301 atagggtgac cggccgatcc ggccgccgct gcaactgcgc cagaatcatg cttcgcacgg
      361 gcgcgatgcc cgttcccgtt gcaacgaaac acaaatcctt cgtcccgtcg tcccggaggt
      421 aaaacgcgcc agccgggccc ttgaatgacg tccggttccc ttcacgcagg ctaaacaggt
      481 aattcgaacc gggcccgccc tgaacgagat tcaggacgag gaggattcgc tctcgctctt
      541 ccggcggtga cgcaatggaa tagggcctgg tcacagagcg tggatgccct tcctttggca
      601 cctcgaacga gacgaattgc ccggctttaa acgtgatact cgccggctcc agcaggcgca
      661 gttcaatagc ccgcacgtca tgcgtcagat cgtgaatgcg gctcacttca gccgtgaagg
      721 tattcacagc agccaacctc tgtacaggtg atgatgctga aagatccgga tccggtcagc
      781 cacgcccgga gggaagcaag agatgcgtct catacgtcat gataccaaga aatgcggcga
      841 ggccgaggta gtacagacca tagttcaccc tggtcgagag cggcacgtcg tagtaacgct
      901 cgggcaagcc atggggaccg ccacggaacg tggacatcac atgcgggatc gccatgatcg
      961 cgatcaagag cagcatgggg ctatggttca tgatgaacag accgatcagg atcggcacac
     1021 ccacccacca gactttgggt gagataatcg ccgtgatacg tccgccgtcc aagggggaca
     1081 aggggatgag gttgaacaga ttgatcatga acccagcgta tgccagcgcc agcagcaatt
     1141 ggctttgggt atattcggct gcgtaatagc aggcaatcgc cgccacggta ccggcaaccg
     1201 gcccggcaat tcccacatag gcctcggttt ccacatccat cggctgctct ttgagttgaa
     1261 tccaggcacc gacgaaggga atgaaggtcg gggctcccac attcaggttg cgctgtttgg
     1321 cggccgcgta atgccccaac tcgtgaaaca gaatcagcag cacgaatcca accgcgtacc
     1381 accagccgaa gaaaaaacta tacgccacca tcgacagcag catggttccg ccggtcaggg
     1441 cgatcttgcc gaatttgagg ccgccgagga ggagcaggag aatcttcacg atgacacacc
     1501 tccgcctccg cctggctgct ccgcctgcgg tttcttcttc aagtatttgg cggccaaccc
     1561 gccgagcgcg atgaccaaca gcatgaataa cttctttccc gccaccaaca agggaatgat
     1621 gaacgcccac agcttcgcca acaggccgga cttgacggct gcgccagcga tcagcgccgt
     1681 caggccgacc gccgccacct tgtccgtcgt gctgttgaag tcggtatatc gtttcccttc
     1741 cacgaactcc acgttggcca gcagttggct gacatgcggt ttcagcaccg ggagctgctc
     1801 cagcgacccg accatgttca tgctgagata cccctgtcgt cccaacgcca gcgtgttgta
     1861 attgacccct accggcgatt ccggctcctg cgccgagatc gcccacacca ccttgttcgc
     1921 cgccttgtcg taatgcggct tctcttccca gccgcgaatg attaacggcg gaaacccttg
     1981 cgcctggcgg tttttgttgt cttcctccgt gccttctttg atggacgtca tcagcgcgtc
     2041 ggcatcccaa ttggccgcgt cgtcgtcttt gacgtagccc gcgtcgatat atcgcacgac
     2101 catgaaccac tgctcccctt caccggtcgc cgtgatcaac cccagttcgg agccggaagg
     2161 gaagtttccc attctcttca acaacgcttg ggtctcttgc gcaccaagga attggtagcc
     2221 tttgggaagc ttcagcaacg cctgatcgcc caacttcgct tcggtgggcc cctcaagcca
     2281 atgaaggcca gacggtttcg gctcctcggc taacgccgga agcgccacgc cggcgaccat
     2341 ccaacacaga aaggccgtca tcagacgcca atggcaccat gtcatggctt cgtctccttc
     2401 gaggatggag caacggaacg tgcttcaggc tgtaccatcg cttagatggc gtcctgcttt
     2461 cctgggactg cgattcatac aaacattcat catcctacct gaatacaccg tagaagtggc
     2521 tcccctttgg tcttaggatg gcattgcgaa tgatcttgcc tacgctattc tctcgcttcg
     2581 ggcttcacaa tcttccgtac ccacacctcc gcgaccagca aggcgttagc gggaatgaga
     2641 cttggcagcc ccttgtcccg ataagccagg tgcggactga tgcgaacttt gcggtagccg
     2701 cctactctca ttccaagcaa cgcctgctcg attccaggaa tgacctgccg ccgtcccagt
     2761 tcgacctggt gatcgacgag cgttacgccg ccatcgactc tcagcatgtg gcccggcaga
     2821 gtcgccgcct gccgttcatt gagcggcacc tcatcgccgc aatgcaggaa cagacgacag
     2881 ttgtacagaa cacgatcccc ccgttgcgcc tccggacctt caccttcacg ctggtcaagg
     2941 agtacgaggg ccttcgaacg tttcatcgag actagtacac aaccgtcgtc agagacagcg
     3001 gcccgacggt cgtcttgttc agcaccttga ccgcgacccg gccgatgacg cggccgtctt
     3061 ccgcttccac atcgacccgc caatcccccg gatcgagtcc ctccttgaag gtgtaggcgc
     3121 gataccctcc ctcgcgcccc ccggaaatct tgagcggaat gcggtcggcg tcggcgaagg
     3181 gctgaccggc atcaggccgg taataccaat gatgataaat cgtcgtttta agggcgaccg
     3241 gggcaaacac cgccgtgaaa caatagaccg ggtcgtccgc cgggatgacg ttgtccgagg
     3301 ttttccacac ctcgtaccag gatttctcaa aactcagctc ataccggtcg ctcgagcgct
     3361 tcacttcgtg atacatgccg ccgaacttca gcgagagggg cacgggcgga atccagttca
     3421 taaaatagaa gccgaccagc agtgcaatca agccaacggc cggcgcaccg gcccacatcg
     3481 cttcccgcgt cgtacgatca gcgttcttcc aatagatcaa ctgcacaacg cgcaacacga
     3541 tcaggactgt caccatggct ccggcgagaa agatcaccgc attcatccag ccggtcatga
     3601 cgggcaggaa aaacgtgaag aaggcaaaac agacgagagc gtagagactg accagcaact
     3661 gtatgttcga tagtcggctc cgtaggaatt cgttcgccac gagaaacgcc accaggacgc
     3721 agaaaaagac ggccgtcccg gcgaaggtcg cgctcttgga ataaaagatg gcgtaggcgc
     3781 tgaagaggcc gcccagcagg aactggatgg ccatcggtgc atagggcttg gcctgcacga
     3841 tccatttcat gaagaagggc gcgtcgggtg gaagatcgtc caccgtggct tcctgggttc
     3901 ccaaacgccc ggtcagtacg atcagaaagc ctaacagcgt caggtaggcc agcaagagca
     3961 agttgtcgcg aagacggtca atcctggtga gcaccagggt gtcgtaagtc acgccgctaa
     4021 agaaaaacag cggcggcagg tacggcttac ccagaaccga ttggaaccgt tgtaatgaga
     4081 ccatgcgcta gatttcagga gacagaggcg attgtcaaac gaagagagga atcaggcgat
     4141 gaaattggaa ggcacgatcg
//
LOCUS       DAOQEW010000282         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9402_length_4147_cov_9.504399, whole genome shotgun sequence.
ACCESSION   DAOQEW010000282 DAOQEW010000000
VERSION     DAOQEW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_4147_cov_9.504399"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..361)
                     /locus_tag="PLS35_18580"
     CDS             complement(14..361)
                     /locus_tag="PLS35_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20734.1"
                     /translation="MKVWAFDENRADFVDAAPERMNADATGYYIIRRGDDVLVREHGQ
                     ERPMPERLTVAGVPFDREQFEHTHPLPLRAEMKRDGQWARAQLQARDRAEIALPLELP
                     IWVTKTPLTEGKA"
     gene            complement(586..750)
                     /locus_tag="PLS35_18585"
     CDS             complement(586..750)
                     /locus_tag="PLS35_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20735.1"
                     /translation="MDTAAEQGMTPEMFAQACAKGMAEQERRAMQEQAARDADPKQPQ
                     EDSPVSTPTP"
     gene            complement(982..1401)
                     /locus_tag="PLS35_18590"
     CDS             complement(982..1401)
                     /locus_tag="PLS35_18590"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNI20736.1"
                     /translation="MKDTLGTAAVGLLFGFCLHRIGFGSWDEVHKMFTIASLRLFLGF
                     GCAVALLALGFALIAKLQRPKWAPRPIHKGTIPGGILFGIGWALCGACPSIAFVQLGD
                     GQWLSLLSLAGIVLGNALYAPIHARWLRFDSGTCASD"
     gene            complement(1398..2012)
                     /locus_tag="PLS35_18595"
                     /pseudo
     CDS             complement(1398..2012)
                     /locus_tag="PLS35_18595"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
     gene            complement(2435..3901)
                     /locus_tag="PLS35_18600"
     CDS             complement(2435..3901)
                     /locus_tag="PLS35_18600"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNI20737.1"
                     /translation="MLPCSAAQRQQIGHSATARGRYQRREPEKTLLHRIVRENLATFL
                     VEAQDRYPSGELPGFIRAEFERYLRCGLLCHGFARIRCPTCHDELLVAFSCKNRGLCP
                     SCASRRMADFAAHLRDRVLPAVAVRQWVLTLPMQLRFLIAWRPKLIGLTLTLFLRALF
                     AWQRRCAHRQGIAKPLCGAVTCIQRFGSSLNLNLHFHTLVPDGVFSETSDGNVQFHPL
                     PPPAVPDLERIARRLIPRLLKKLATASEQNEAADGMNLLTQYLQAQSQRTEPAMERPR
                     GLRIFAEGFSLHAGVSVTELDGDALERLARYCARPPLSLHRIAVEPDGQILYRAKHSA
                     PGTPRRLHLSATQFLGRIAALIPPPRSHLLRYHGVFAPHSKHRARIVPQCPRATPASS
                     TTAPHRPSRSRSRLDWASLLRRVFALDVLHCERCGGRRHLLSMITEAKTAQKILAHLG
                     LPTQAPAQSPARAPPPIPDSADWSDQAGVDPIPSSWSS"
BASE COUNT          857 a         1203 c         1344 g          743 t
ORIGIN      
        1 acgggcgaca ggatcacgcc ttgccctcgg tcagcggcgt tttcgtcacc caaatgggca
       61 attccagcgg cagggcgatt tcggcccggt cgcgggcctg caattgcgcc cgcgcccatt
      121 ggccgtctcg tttcatctcc gctcgcagag gcagggggtg ggtgtgctcg aactgctccc
      181 ggtcaaatgg caccccggct acggtcagcc gctcaggcat gggccgttcc tggccgtgct
      241 cgcggactaa cacgtcatcg ccgcgccgga tgatgtagta cccggtcgca tcagcgttca
      301 tccgctccgg cgcagcatct acgaaatcgg cccggttctc atcgaatgcc cagactttca
      361 tgcgcaaccc tcccgaatcg acatcagatg gtggcaggaa tgtggctgca ttgtggtgaa
      421 gtgccacgct attgccaccg tatgcacaca cagccgccac agtgtcgcca ctgtcttacc
      481 aatatcccgc caccatcgtt ccaccatgtc accaccttgt taccgtcatc attgacacca
      541 tgccgccacc gtctttccac actgttccca ccattgccgg tggcatcatg gggtgggagt
      601 agaaacaggg gagtcctctt gtggctgttt gggatcggca tccctcgccg cctgttcctg
      661 catagcccgc cgctcctgtt cggccatgcc ttttgcgcag gcctgagcga acatttccgg
      721 cgtcatgcct tgctccgcag cggtatccat gtacgccgcg aaacgtgctt tgagtccggc
      781 aagcggtgcg gccagaacgg tcaagctgag ggaggttagg aaaagtaaac gcggttcggg
      841 tgccatggac ggggaagtca tggcgtgcat cagagatcga tcgctgacga cacgcgacag
      901 tttactctaa actgtcgccg atctggctcc ggtcttccgc ttggccgccg gggttcttga
      961 ggacggggtg ttttggacgg cttagtcgct tgcgcaggtg ccgctgtcga atcgcagcca
     1021 gcgggcatgg atcggggcat acagcgcgtt gcccagcacg atccctgcaa gcgatagcag
     1081 ggacagccac tgaccatcgc ccagctggac aaatgcgatg gatggacagg ctccgcacag
     1141 ggcccagccg atgccaaaca ggatgccgcc gggaatcgtg cctttgtgga tcggacgcgg
     1201 ggcccacttg gggcgctgaa gcttggcaat cagcgcaaag ccgagcgcca agagggcaac
     1261 cgcacagcca aagcccaaaa acagtcgcag gctggcaatg gtaaacatct tgtggacttc
     1321 atcccaggag ccaaagccaa tgcgatgaag acagaagccg aacagcagtc ccacggcggc
     1381 ggttcccagt gtgtccttca tcgcaaggcc cccagtagga gcgaggtcag gatgcctgcc
     1441 cccaagaacg aggccgtggc cagcaagctt ccgggctgaa agcgactgac tccgcacagg
     1501 ccgtgtcctg aggtgcagcc gcccgccatg cgggtgccga agccgaccag cagtccgcca
     1561 ccaaatagaa cagtgccaag tgccaagggc tggtgtccaa acgtcgcaaa gaacagtgcg
     1621 cgatccgagt gggcccctgc aaggtgagca gagagcgccg cgcccagtgc gatggccgca
     1681 aagaacagaa ggtgaacggg agcgctctgc ttgggcttgg gcagaagctg cggagctggc
     1741 tgggcttggg ccgccagctc tgcatccagc gtggcggctt gttcttcgga cagaccaaac
     1801 tctgcgatgg tggctgcgcg cagggcttcc atcagctcat cttggggcag gctgctttgg
     1861 gaatccgagt cctttttgcg aagtcgcagc gcatccgtta gcgcagtgaa gcgaccagag
     1921 acggcgaaca tccgtccaag cagcagccag tgaatcagtg caacccccgc cagagccagc
     1981 gcacagagcc agaggggcca gtacgtcatt ttaagacctt ctttaggagc actccgctcg
     2041 gaagtagatt atcatgaacc cactcctcgc ggaggcttcg ctgatagcgc gcgcagcggg
     2101 tgtagtcgct tctacagtag ttggtcttcc acacagccag cgttccgctc atggaaaaga
     2161 gcggaaacaa tcggcagctt gcttcgtgag gacactgcgg catcttgtcc cacctgaggt
     2221 taggaaaagt aaacgcggtt cgggtgccat ggacggggaa gtcatggcgt gcatcagaga
     2281 tcgatcgctg acgacacgcg acagtttact ctaaactgtc gccgatctgt ggggaaaata
     2341 acaagtgatt tcttctctga tgggtcggtg gataggccga tggcaggggg ctcgctggcg
     2401 agccgggaga tgtcggtgcg gcaagggggc gagcttagct ggaccagctc gatgggattg
     2461 gatcgacccc tgcttgatcg ctccaatcgg ctgaatcggg gatgggtggg ggcgctcggg
     2521 cgggcgattg ggcgggagcc tgggtcggca ggccgaggtg agcaaggatt ttctgggcgg
     2581 tcttggcctc ggtgatcatg gagagcaggt ggcggcgacc gccgcagcgc tcgcaatgaa
     2641 ggacatcgag agcgaacaca cggcgcagaa gcgacgccca atcgagacgg gaacgtgacc
     2701 gcgacggtct atgaggagct gtggtggagg acgcgggcgt tgcgcgtggg cactggggaa
     2761 cgatgcgagc ccggtgtttg ctgtgtggcg cgaacactcc gtggtagcga agcaaatgag
     2821 agcgaggtgg aggaatcagc gcggcgatgc gaccgagaaa ctgcgtcgct gataagtgca
     2881 gtcgcctggg tgtgccaggg gcgctgtgct tggctcgata gaggatctgg ccgtccggct
     2941 ccaccgcgat ccgatgaagc gacaggggcg gacgagcaca gtagcgagca agccgctcga
     3001 gcgcgtcgcc gtccagttcg gtgacggaga ccccagcatg gagcgagaac ccttcggcga
     3061 agatacgcaa accgcgaggg cgctccatcg ctggctctgt cctttgcgac tgcgcctgca
     3121 ggtactgcgt gagcaggttc atcccgtctg cagcttcgtt ttgctccgac gccgtagcga
     3181 gcttcttgag caagcggggg atcaagcgac gggctatccg ttccagatcc ggcacagcgg
     3241 gaggagggag cgggtggaac tgaacatttc cgtcggaagt ctcggaaaat accccgtcag
     3301 gtaccagcgt gtggaagtga aggtttaagt tgagggatga gccaaatctt tgaatgcaag
     3361 tgacggctcc gcagagcggt tttgcgatgc cttgccggtg agcgcagcgt cgctgccaag
     3421 caaacagtgc tcgcagaaac aacgtcaaag tcaagccaat gagcttgggt cgccaagcga
     3481 tcagaaagcg gagctgcatc ggcagagtca agacccactg ccgcaccgca acggcgggaa
     3541 gtacacggtc ccgaaggtgt gctgcaaagt cggccatccg ccggctggca cacgatggac
     3601 agagccctcg atttttgcag gagaaggcca cgagcagctc atcgtggcac gtcggacagc
     3661 gaatacgggc aaagccgtgg cagagcagcc cgcagcgcaa gtagcgctca aactcggcgc
     3721 gaatgaagcc aggtagctcg ccgctgggat agcggtcctg cgcttcgacg aggaaggtag
     3781 ccaggttttc gcgaacgatg cggtggagca gggtcttttc tggctcgcga cgctggtagc
     3841 gcccccgagc agtggcagaa tgaccgattt gctgccgctg cgctgcggaa caagggagca
     3901 ccagcccccg ccaagcacaa caggtgccag acgcctactg acatgttctg cgtcaggaac
     3961 gcacaacaca ggggagctgc ggtcgtccgg gataccggat gctgtccgaa gctcggacac
     4021 cgcactgcct acctcgctcg tccgcaggat cggaccccct tgccgaaaga ctccatgatc
     4081 gcaagtctgc gcactttgtg cgacgataga ttactgaaca tggtaaattc ttcctgacgt
     4141 acccttc
//
LOCUS       DAOQEW010000283         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9451_length_4137_cov_7.361832, whole genome shotgun sequence.
ACCESSION   DAOQEW010000283 DAOQEW010000000
VERSION     DAOQEW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9451_length_4137_cov_7.361832"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLS35_18605"
     CDS             complement(<1..1126)
                     /locus_tag="PLS35_18605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20738.1"
                     /translation="MGSTIFVIDNSPAVQRMVEQLSAPDGHRVLAFQDGPSALEAARS
                     QSPALIIADYHLEQMTFSGFCKEIGRQDNLAETLIVSIVDSADRLDESKLRALGVRAF
                     LKKPFQREQLLETIQSILSKPEGSRSQKPAKSRTWPPATTGADDDDETASDASAEHTS
                     RQAEKKEQAPMSSTAPATRPTGEDLMKGLVEHLLHSTTVKADRAITELLPAAVAKEVA
                     GQLGAALSKAVQAEVSKQVAEALAPERIQNTLREMVQMELKRQSESQRAGMETSIRQA
                     VTELAPGLVAQSAEKLLPGLTDAGVKQHLPEALQSQRDLITDGVKKEVEQVAATYARQ
                     AAEDIVRELAKDPILQAVQRVVPDVAETQIRAEITRLSSPD"
     gene            1339..2064
                     /locus_tag="PLS35_18610"
     CDS             1339..2064
                     /locus_tag="PLS35_18610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNI20739.1"
                     /translation="MSQSRIQVVFFDAADTLFHIQGSVAEIYLQHAERHGFRKTPDSL
                     AAIKAAFARSFRDAPPPVFAATEPGGIKQSERLWWFDIVHNVFYRVGMFEKFDEFFED
                     VFARFAQAESWKLFPETLDVLKTLKDHGYELGIISNFDSRLFSVLRGLGIADFFDTVT
                     ISSLAHAAKPSARIFEQALEKHAVDPEDALHVGDSARDDVKGAIAVGLTGVLLARGLP
                     PEGSNGATIATLNELLPLLSRLQ"
     gene            complement(2050..2781)
                     /locus_tag="PLS35_18615"
     CDS             complement(2050..2781)
                     /locus_tag="PLS35_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNI20740.1"
                     /translation="METGSTLGLVRHKLSTARSVTILTGAGISADSGVPTFRGADGLW
                     RHYRAEDLATPEAFARDPRLVWEWYNWRRELIATKRPNPAHEAVASMEQRFKQFWLIT
                     QNVDGLHGDAGSRQLSEIHGNLWKVRCTRCRDIVENRGVPIAMLPLCRSCGGLLRPHI
                     VWFGESLAEEDLDRSAAALQSSDVCLIVGTSGMVYPAAGFGAIAKQAGAFIVEINLDA
                     TPHSNLADATLQGRARDIVPLLLEA"
     gene            2866..>4137
                     /locus_tag="PLS35_18620"
     CDS             2866..>4137
                     /locus_tag="PLS35_18620"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNI20741.1"
                     /translation="MKQIMVVGAGSVGGFFGAHLAKNNPNVSFLLRPRTLEAVKRNGL
                     TIKSAKGNFTVHPPAASDPRELATPDLIILAVKAYDLDEVMTQLEPVLTDRTVILTLQ
                     NGIDTEDRIISRLHRDCVVGGVAFIYSKIVEPGVIEHYKRGGVAIGELMGHKSERVSQ
                     IADIFKQAGISCQLSEDIRKSKWEKMCWNCVFNPLTVMIDDKVAKALDHPEMAGVIRQ
                     IVGEVAAVSAAVKVPLAPDMAEKVVKWTQELRDIHTSMYDDWKAKRPTEIDYLNGYIV
                     RVGRELGIPTPVNEALTAMVKTITEKELSGPSIVRIDGAVVQPVSLTRTALGQLPREQ
                     RVDDISELMPSMRGRAIRVKGLLEVPALAVDADHVTFHSVDGKYAATLTLQQAQDFGL
                     LLYELDGQPLPDGKGGPYRLVTPGLGDLCANV"
BASE COUNT          772 a         1175 c         1314 g          876 t
ORIGIN      
        1 agtcgggtga gctcaatcgt gtgatctcgg cccggatctg agtttccgcc acgtcaggaa
       61 cgacccgttg caccgcctgc aggatcgggt ctttcgccaa ttcccgcaca atgtcctcgg
      121 ccgcttggcg tgcataggtc gcggccacct gctcgacttc ttttttcact ccatccgtga
      181 tcaggtcgcg ttggctctgc aaggcttccg gcagatgttg cttcacgcca gcgtccgtga
      241 gccctggaag taatttttcg gcagactgcg ccacgaggcc aggcgccaat tccgtcacgg
      301 cttgtcggat gctggtctcc atgccggcac gttgcgactc gctctgacgt ttcaattcca
      361 tctgcaccat ttcgcggagg gtgttctgga tccgttccgg ggcgagcgct tccgcaacct
      421 gtttgctcac ttcggcctgc acggccttgc tgagcgcggc ccctaattgg cccgcaacct
      481 ccttggcaac ggcggcgggc aacaactcgg tgatggcccg gtcggctttg acggtcgttg
      541 agtggaggag atgttcgacc agccccttca tcaagtcctc gcccgtgggt ctggttgcgg
      601 gtgctgttga cgacatgggg gcctgctcct tcttctccgc ctgtcgggag gtgtgttcag
      661 cagaggcatc cgacgcggtc tcgtcatcgt cgtccgctcc cgtggtggcc ggaggccagg
      721 tgcgagactt ggctggtttc tggctgcggg atccttccgg tttgctgaga atgctctgaa
      781 tcgtctcaag gagttgttcc cgttgaaaag gctttttgag aaacgcccgc acgccaagcg
      841 cgcgcagctt gctttcatcg aggcgatcgg cactatcgac gattgagacg atgagcgttt
      901 cggccagatt gtcctgtcgt ccgatttctt tgcagaatcc ggaaaaggtc atctgctcga
      961 gatggtaatc ggcaatgatc agcgcggggc tttgtgatcg ggcggcttca agggcggagg
     1021 ggccgtcttg aaacgccaat acccgatgcc catcgggggc ggagagttgc tccaccatgc
     1081 gctgcacggc cggactgttg tcgatgacga agatcgtgga acccattgga aacacctcgc
     1141 tctacccact ccagcgaagc taacaaaggg tctgaaaact gtcaaagaaa acgccgaatc
     1201 caactgttct gacgaggcag gggtgggcct cgggcgctca gcgctggcgg aggttttacg
     1261 catcctgata caatccgccc accgtgccgg ttggcgggtg ctccgtgagt gccgaagaga
     1321 gaggagtgaa tccatacgat gagtcagagc cgcatccaag tcgtgttttt cgacgctgcc
     1381 gataccctct ttcacattca aggatccgtg gcggaaattt atctccagca tgcggagcgc
     1441 cacggctttc gcaaaacgcc tgactcgtta gcggcaatca aggcggcgtt tgcccgctcg
     1501 tttcgcgatg cgccgccgcc ggtatttgcc gcgacggaac cgggcgggat taaacaatcg
     1561 gagcgcctgt ggtggttcga tatcgtccac aacgtctttt atcgcgtcgg catgttcgag
     1621 aagtttgatg agttttttga ggatgtgttt gcacgttttg cgcaagcgga gtcctggaag
     1681 ctgtttccgg agacgctgga tgtgctcaag accctcaagg atcacggata cgagttgggg
     1741 atcatttcga attttgactc gcggctgttt tccgtgctgc gcgggctcgg gattgccgac
     1801 ttcttcgata cggtcacgat atccagcctc gctcacgccg cgaagccgtc cgcgcgaatt
     1861 ttcgagcagg cattggagaa acatgccgtc gacccggagg acgcgctgca tgtcggcgat
     1921 agcgcgcgtg atgatgtgaa gggcgcaatc gcggtcggac tgacgggggt attgctggcg
     1981 agagggctgc cgccagaggg atcgaacggt gcgacgatcg caacgttgaa cgaactcctg
     2041 ccgctcttgt cacgcctcca gtaacaatgg cacgatatct cgcgcacgcc cctgcaacgt
     2101 ggcgtcggcc agattcgagt gcggcgtcgc gtcgagattg atctccacga taaacgcgcc
     2161 cgcctgcttg gcgatggcgc cgaatcccgc cgccggatag accatcccgg acgtaccgac
     2221 gatcaagcag acatcgctgc tctgtagggc ggcggcactc ctgtccagat cctcttccgc
     2281 cagggattcc ccgaaccaga cgatgtgcgg gcgaagcagt ccgccgcaag accggcagag
     2341 gggcagcatg gcgatgggta cgccccgatt ttcgacaatg tcccggcatc gggtgcagcg
     2401 gaccttccag aggttaccgt gaatttcgga taactgccgc gagccggcgt caccgtgcag
     2461 cccgtctaca ttctgcgtga tgagccagaa ctgcttgaag cgctgctcca tcgacgccac
     2521 cgcttcgtgt gccggattcg gtcgtttggt ggcgatgagt tcccggcgcc agttgtacca
     2581 ttcccacacc aggcgcgggt cacgcgcgaa ggcttccggt gtggcgagat cctctgcgcg
     2641 atagtgtcgc cagagcccgt cggcgccgcg aaatgtcggg acgccgctgt cggcggagat
     2701 cccggctccg gtcaggattg tgacagaccg cgccgtggag agtttgtggc ggacaaggcc
     2761 gagagtggat ccggtttcca taaaggtcag tctcccctca ctgtttcagg tgcgcggctg
     2821 catgctatag tacgcgtctt ttgaacgcaa cgagtgaggc agctgatgaa gcagatcatg
     2881 gtggtcggtg ctggttccgt cggtggattc ttcggggcgc atctcgccaa gaacaatccc
     2941 aacgtgtcat ttcttcttcg gcctcgcacg ttggaggcgg tgaagcggaa cggcctcacg
     3001 atcaagagcg ccaagggaaa tttcaccgtc catccgccgg ccgcctcgga tccccgggag
     3061 cttgcgacac ctgatctcat catcctggcc gtgaaggcct acgacctcga cgaggtcatg
     3121 acgcagttgg aaccggtgct gacggatcgc acggtgattc tcaccctgca gaacggcatc
     3181 gatacagaag atcgcatcat atcccgcctg catcgggact gcgtggtcgg cggggtggcc
     3241 ttcatctatt cgaaaattgt cgaaccaggc gtcattgagc attacaagcg cggcggggtg
     3301 gcgatcgggg aactgatggg ccacaagagc gagcgtgtgt cccagatcgc cgacattttc
     3361 aagcaggcag ggatttcctg ccaactgagc gaagatatcc ggaaaagcaa atgggagaag
     3421 atgtgctgga attgtgtctt caatcctctc acggtcatga tcgacgacaa ggtggcgaag
     3481 gcactcgatc atccggaaat ggccggcgtc atccggcaga ttgtcggcga agtggccgcg
     3541 gtgtccgcgg cggtgaaggt tccgctggcg cctgacatgg ccgagaaggt ggtgaagtgg
     3601 acgcaggagc tgcgtgacat ccatacgtcg atgtacgacg attggaaagc caaacgcccg
     3661 acggagatcg actatctgaa tggctacatc gtgcgggtgg gacgcgagtt gggtattccc
     3721 acaccggtga atgaagcgct gacggcgatg gtcaagacga tcactgaaaa ggaactgtca
     3781 gggccgagca ttgtgcgcat cgacggcgcg gtcgttcagc cggtctcatt gacgagaaca
     3841 gcgctggggc aattgccgcg ggagcaacgg gtggacgata tcagtgagtt gatgccgtcc
     3901 atgcggggtc gcgccatccg cgtgaagggc ctgctggagg ttccggcgct tgcggtggac
     3961 gcggaccatg tgacgtttca ctccgtcgat ggcaagtatg ccgcgaccct cacgctacag
     4021 caggcgcagg acttcgggct gttgttgtac gagctggatg ggcagcctct tccggacggc
     4081 aaaggtggcc cctatcgttt ggtgacgcct ggtctgggcg atctctgcgc caatgtg
//
LOCUS       DAOQEW010000284         4106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9560_length_4106_cov_5.721550, whole genome shotgun sequence.
ACCESSION   DAOQEW010000284 DAOQEW010000000
VERSION     DAOQEW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9560_length_4106_cov_5.721550"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1281)
                     /locus_tag="PLS35_18625"
     CDS             complement(55..1281)
                     /locus_tag="PLS35_18625"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNI20742.1"
                     /translation="MDWPSDSPHALLDPPRSTVETLPADQPVTLFDYWDVLVARRKMI
                     AAFFLFGTCLTLAISLLLPKIYESTSSVLPQLESKEGGALAALLTAPGAAGMAQNLGI
                     GLPGLPTTPTDVFVSILKSRLMADEVIKKFDLMNRYHESTMVDTRKELEDHLRITVTK
                     EKVIKVSVEDEDPQVAADIANFYVANLDRLNRTVTVSKAGQNRAFLERRLAETMESMA
                     KAEEALRDFQAKNKTVAVEAQAKVMIEAAAMIQGQITAQEVQLQVMGGYLSPDNPDLA
                     RIRSNVEELKKQLAIMGSGKDARGLLSSDRLHPAMVAVPDLALQFGRLFRQVKVQETL
                     FTLLTSQHEQAKIAEARDTPTVQVLDPAVPADKRSRPRVLLNIAVAGVLALIMGTFTA
                     YFLDYRARVRRISTPA"
     gene            complement(1298..3817)
                     /locus_tag="PLS35_18630"
     CDS             complement(1298..3817)
                     /locus_tag="PLS35_18630"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNI20743.1"
                     /translation="MVLPPSLLAQTLTNPAIPSSPSGASASPSIGSQISPFLPFGTYG
                     LPTAPGQAIVTNPTATQPIVPQHAPCPIPTAPDLSPENTVPNLNDYWPMTANSLLPTS
                     VEQRMRQEEEERDRQLERVQAEKEKRGLELQSQVEREKKGVSQLQGLQSAMQGAVGGM
                     QGQVQNQLNQPKQPLEQKQFTAELLRHQDFSVEEAFAQFSVLQGVKSRLKQFGYDFFD
                     AHAGGFAALQDVPVGPDYVIGPQDSLAVHIWNVPDQSFNRSYIAPVERDGMVIIPQVG
                     AIPVGGQTFSQAERTIHARLSMLLKRFELHVSMARIRTMKVYVVGEVARPGAYELSAL
                     ATASNAIYAACGPSRSGSLRQVRIMRDGKTIGQLDLYEFLLQGDRRQDHRLQAGDVVL
                     VPPLGSVVAISGSVKRPAIYELKPGTRLTELLTLAGGLTPLSDRQRCHLFRQDPALQE
                     RNMIDVDLVRAFASQGAEKSRPGVEGGDPLLMDGDYIRIATLPTQVVNVVSLVGAVKS
                     PGPYEFRAGMRVKDILTPEQLTVDAYADRAEIVRTDPATYLTKVIPFSPKAVFEGRES
                     DNVALSRLDQVVISSQMRAPALVLVEGEVRRAGYFTIEIGERLSSVLKRSGGFTPNAF
                     PNGIVLVRESVKVKQQAELDRFIAAERQRLTAQSAGIAAGTAGLNVMAGASGAMAEQQ
                     VLALRLQQLEATASRIELGRVIVSLDSIERLEGTEDDIILESRDRITIPTPPQTVGII
                     GSVKSPTSVVYRPGLDLNDYLRQAGGVTEDANKREMYVMRANGTTDSSYLSAKEMRPG
                     DTIVVPQKIEARPPQLALWQTVASIIGSLALTAAGIAVVGR"
BASE COUNT          922 a         1230 c         1070 g          884 t
ORIGIN      
        1 ttctagcaga gaaatcgcgc ggattctacc attgcgactg aaaaatcgtc aatttcacgc
       61 aggtgttgaa atccggcgaa cccgagcacg atagtcgagg aaatacgcgg tgaacgtccc
      121 catgatcagt gcgagcaccc cggcgacggc tatgttgagc aagacacgtg gacgacttcg
      181 tttatctgcc gggacagccg gatccaacac ttgcactgta ggggtatcac gagcttccgc
      241 aatcttggcc tgttcatgct gcgaagtgag tagcgtaaag agcgtttcct gcactttcac
      301 ttgtcggaac aatctcccga actgcaaagc caggtcgggt accgcaacca tggctggatg
      361 aagccgatca ctggacaaga gaccccttgc atctttcccc gaacccatga tggccagttg
      421 cttcttgagc tcttccacat tagaccggat tctggctaaa tcgggattat cgggcgagag
      481 ataccccccc atgacttgca attggacttc ctgtgccgtg atctgtcctt gaatcatcgc
      541 ggcagcttcg atcatcacct tcgcttgcgc ctcgacggcg accgtcttgt ttttggcctg
      601 aaagtcccgt agcgcctctt ccgccttcgc catactttcc atggtttcag ccaatcgccg
      661 ttcaaggaac gcacggtttt gcccggcctt gctgaccgtg accgtgcggt tcagccgatc
      721 aaggttcgcc acataaaaat tggctatatc cgcagccact tgaggatctt catcctcaac
      781 cgacaccttg atcactttct ccttcgtcac agtaatccgc agatggtcct ccaattcctt
      841 tctcgtatcg accatcgtcg actcgtgata tcgattcatg agatcgaatt tcttgataac
      901 ctcgtcggcc attaaacggg atttcaaaat cgaaacgaac acatcggtcg gtgtggtcgg
      961 aagcccgggc aggccaatac cgagattctg tgccataccg gcggcacccg gtgcggtcaa
     1021 gagggctgcc aaggccccac cctctttgga ttccaattgt ggcaagaccg atgacgttga
     1081 ttcgtagatt ttcgggagaa gaaggctaat cgctaacgtg aggcacgtcc cgaataaaaa
     1141 gaacgcggca atcatcttac gtcgcgccac caggacatcc caataatcaa acaatgttac
     1201 gggctgatcg gcaggaaggg tttcaaccgt ggagcgcggc ggatccaaaa gggcgtgggg
     1261 gctatccgaa ggccaatcca tagacggatc cgatcgacta tcggccaacc acggcaatgc
     1321 cggcggcggt cagagcaaga ctcccaatga tactggctac ggtctgccac aacgcgagct
     1381 gagggggacg cgcctcgatt ttttgtggca ccacgatggt atctccgggg cgcatttcct
     1441 ttgccgagag ataggaggaa tccgtcgtcc cattggcccg catcacatac atctcacgct
     1501 tgttcgcatc ttcggtcacc ccaccagctt ggcgcaaata gtcattcaga tccaagcctg
     1561 gtcgatagac caccgaagtg ggactcttga cggaaccaat aatcccaacc gtttggggcg
     1621 gtgtcggaat ggtaattcgg tcccgggact ccaaaatgat atcgtcctcc gtcccttcca
     1681 gacgttcgat cgaatccaaa cttacgatga cacgacccaa ctcgatccgt gatgcggtcg
     1741 cctccagttg ctgaagccgc aaggccagca cctgctgctc cgccatggct ccactggcgc
     1801 ctgccatgac gttcaacccg gcggtgccgg cagctattcc ggccgattga gccgttaatc
     1861 gctgccgttc tgccgcgata aaccgatcca gctcagcctg ctgcttcacc ttgaccgact
     1921 cccgcaccag gacaatgccg ttcggaaacg cattgggcgt aaagccgccg gagcgtttca
     1981 gcaccgagct caaccgctcg ccgatctcaa tggtgaagta tcccgctcgc cgaacctctc
     2041 cctccaccaa caccagcgcc ggggcgcgca tctggcttga tatgaccacc tggtccagcc
     2101 ggctcaacgc aacattgtca gattcccgac cttcaaagac cgctttagga ctgaagggaa
     2161 tcaccttcgt cagatatgtg gccggatcgg tacgaacgat ttcagcacga tcggcatacg
     2221 catccaccgt gagttgctcg ggcgtcagga tatcttttac gcgcatcccg gcccgaaatt
     2281 cataagggcc aggacttttc accgccccga cgagactcac cacgttgacc acctgagtag
     2341 gcaacgtcgc gattcgaata taatctccgt ccatgagcaa cggatctccg ccctcaacgc
     2401 cgggacggct tttctccgcc ccttgcgacg caaaggcgcg caccagatcc acatcgatca
     2461 tatttcgttc ctgcaaagcc ggatcttgcc ggaaaagatg gcagcgctgg cggtcagaca
     2521 aaggggtcag cccgcccgct agtgtcagca attcggtcag acgcgtaccc ggcttgagtt
     2581 cataaatcgc cggtcgttta actgatccgc tgatggcgac taccgatccg agtggcggaa
     2641 cgagcacgac gtcgccggcc tgaagtcgat ggtcttgtcg ccggtctcct tggagcaaaa
     2701 attcatacaa gtccaactgc ccgatggttt tcccgtcccg cataattcgg acctgtcgca
     2761 gagaaccaga tcgggacggt ccgcaggctg cataaatggc attcgatgcc gtggctaatg
     2821 cactgagctc ataggctccc ggccgcgcca cctctccaac tacatacacc ttcatggtcc
     2881 ggatgcgggc catcgacacg tgtaattcaa aacgcttcag cagcatgctc agccgagcat
     2941 gaatcgtacg ctcggcctgt gaaaatgttt ggccgccgac cggaatagcc ccaacttgag
     3001 ggatgatcac catcccgtct cgctccacag gggcgatgta gctgcgattg aaactttgat
     3061 ccggcacatt ccaaatgtgc acggccagcg aatcctgcgg gccaatcaca taatccggcc
     3121 caacggggac atcttgcaac gcggcaaaac cgccggcatg cgcgtcaaaa aaatcataac
     3181 caaactgctt tagacggctc ttgactccct gcaggactga aaattgcgca aaggcctctt
     3241 ccacggaaaa gtcctgatgc cgtagcaact cagcagtaaa ctgtttttgc tccagcggtt
     3301 gtttcggttg attgagctga ttttggacct gcccctgcat gccccccacc gctccctgca
     3361 tggccgattg cagtccctga agttgagaga cgcctttttt ttcccgctct acctggctct
     3421 gtaactccaa accacgtttt tctttttcgg cctgcacacg ctctaattgc cgatcgcgct
     3481 cttcctcttc ttggcgcatg cgctgttcaa cagaagtagg aagcaaacta ttcgccgtca
     3541 tgggccagta atcgttcagg tttggcaccg tgttttcggg cgacaaatcg ggagccgtcg
     3601 gaatcgggca tggggcatgt tgaggaacaa tgggctgggt cgccgtcgga ttcgtgacaa
     3661 tggcttgccc cggtgcggtc ggcaacccgt aagttccaaa tggaaggaag ggggagattt
     3721 ggctgccgat tgaaggactg gcggatgcac cggaggggga agagggaatc gccggattcg
     3781 tgagcgtctg cgcgagcaag gacggtggca ggaccatacc tcctaccaag aaccagatca
     3841 ccgccctcat ccaccggcct gtgatttgcc cactacgaac aaggggcaca cgccatggca
     3901 ttacaatagg catactgtcc taacgatctc gcctgtggat aacgactgta ggttctaatc
     3961 caaagtcacc ggctcagtca atcgaggccg gaaccagcgc agctagaatt gcacggccac
     4021 agcggtccat aggagatgat ttttcgctgt gacgccggcg gcgaaggttt cagcaaacgg
     4081 cgtaatggcc gacacctgtc ccggat
//
LOCUS       DAOQEW010000285         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9577_length_4101_cov_9.428819, whole genome shotgun sequence.
ACCESSION   DAOQEW010000285 DAOQEW010000000
VERSION     DAOQEW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9577_length_4101_cov_9.428819"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4101)
                     /locus_tag="PLS35_18635"
     CDS             complement(<1..>4101)
                     /locus_tag="PLS35_18635"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNI20744.1"
                     /translation="KVRKARELYDLADAIDATKARKESPEDALPAGWTEASPGGMAAS
                     RDKTTGGIVDKNIATGKWFFVSNLGGVESKDGFDTRDEAFSALADASRMADKGKSANS
                     EESPSAEKQQAAKDLNDALSDLADIFGKKFRANMMPEQEQKLFPVMVRVFDAAFRLGY
                     HSFKDAARFVLQTIREKIGAEVADAVTIDHLQGAYIAMSGGKELATAKRDVVSVESKT
                     ELEQGNVPDERSGTDLERDSENAATENGVGKKGVRDGSGRDGGAGGPGVPGAETTGSS
                     GSGVGIPGPEALADRAASDNEVYVGAPGVSPGASGDSVSQRGGDLRLDGPPIEPDAAK
                     GIGAAATGRPDLARKIALQASAPTDPGASIADALPMLSAGQQEDVAKAERRFAVPDGY
                     GMLFTNGTGTGKTFSGLGIIKRFSNAGKNNILIVVPNDKIIEDWQKSGRMLGLDISEL
                     KNTKDAGKGIVVTTYANMGANSELVRREWDLVVHDEAHHLMENSEARSTNALRTLRAI
                     TLHPDGANDRHAALYADEIMKLADLRARLKNNIEIGKSSSIPVAEKERIAAENARLSE
                     SLALAEKRNRETQKIVREDVESRQGAVRPRALFLSFTPFAYEPSIDWANGYLFDYNEG
                     RPSDKTESRGYNQGSNSDQFFMQHFGYRMRTGKLTQPDAKVDRGLMQRQFNAFLKKKG
                     SLSGRVLDVEADYDRRFLLVESAIGQRIDEALEWFEDQGKKTNDKDRKAALRDVRKVI
                     ADKFDYQSRRYLLEAIKAQEVIPHIREHMAMGRKVVVFHDYNQGGGFNPFDMNERERG
                     AEFSESGDTDLINSVIREFKTEFKDLIGSDLFKASSPIANFQKQFPGVLLVNGLVPAK
                     TRRENVARFQDDASGPQLILVQSQAGKEGISLHDTTGKHQRVLFNLGQPTQPTMAMQQ
                     EGRIYRMGQVTDAIIRYLNTGTNWERWAFATTIAQRASAAENLGSGELARALKDAFIS
                     GFEESGNYRAGMEDEGKGGKARDAAANNVLSEYDRAKAFYFGTQKKDSRTKAQEGTDY
                     YATPEPIGLKMVEFADIRAGDDVLEPSAGHGAIARWIPEHAKRTAIEPSTALRSRLAM
                     VFDGNILGSNFEDLNVVNKFDAIVMNPPFGTGGKTAVDHLAKAATHLRDGGRIVALLP
                     TGPAADKRLDKWLNGGEEVAVKPLVTHPELGPIYKGDTISTQTDTGLVVERMLSADTI
                     VAKSPTMRAETAHLVRNIVKADPTGPRTTESGPSKDVYQIADIQLPAVTFERAGTGVR
                     THIVVLEKQTNKNKLNTVPQKISRDYSDAENINTLFDRIENAFVRPRENAREEESAQT
                     KAPSVGAQGSEKVRPDGGPATVEKMLPGGKLLTDAPKITYTTKAGKK"
BASE COUNT          668 a         1305 c         1144 g          984 t
ORIGIN      
        1 cttcttgccg gccttcgtcg tgtacgtgat cttgggcgcg tcggtgagca gtttcccgcc
       61 cggcagcatc ttctcgacgg tcgccggccc gccatccgga cgaaccttct cggaaccctg
      121 tgcgccaacg gacggcgctt tcgtctgcgc ggactcttcc tccctggcgt tctcgcgagg
      181 acggacaaat gcattctcga tccggtcgaa cagggtgttg atgttctctg catcggagta
      241 gtcccggctg atcttctgcg ggactgtgtt cagtttgttc ttgttggtct gcttttcgag
      301 gacgacgata tgcgtgcgga cgcccgtccc agcgcgctcg aacgtgacgg caggcagttg
      361 aatgtcggcg atctggtaga cgtctttgga tggtcctgac tccgttgtac gcgggccggt
      421 cggatcagct ttgacgatgt tccgaacgag atgtgcagtt tctgcacgca tcgtcggaga
      481 tttcgcaacg atcgtatctg cagacaacat ccgctcaacg acaagaccag tatcggtctg
      541 cgtactgatc gtgtcgccct tgtagatcgg cccaagctcc ggatgcgtca ccagcggctt
      601 gaccgccacc tcttcgccgc cgttcaacca cttgtccagc cgtttatccg ccgccggccc
      661 ggtcggcagc aaagccacga tgcgccctcc atcgcgcaga tgcgtcgcgg ctttggccag
      721 atgatccact gccgtctttc cgcccgttcc gaacggcgga ttcatcacga tggcgtcgaa
      781 cttgttgacg acgttcagat cctcgaaatt gctgccgagg atgttgccgt cgaacaccat
      841 cgccaggcgc gaacgcagcg ccgtgctcgg ctcgatggcc gtccgcttgg cgtgctcagg
      901 aatccatcgc gcaatggcac catgcccggc ggacggctcc agaacatcat cgcctgcccg
      961 gatgtcggcg aactcgacca tcttcaagcc gatcggctcc ggcgtggcgt agtagtccgt
     1021 tccctcctgt gccttggtcc gactgtcttt cttctgcgtt ccgaagtaga acgccttggc
     1081 ccggtcgtac tcgctcagga cattgttcgc cgccgcgtcg cgagcctttc cgcccttccc
     1141 ctcgtcttcc atgccagccc tgtagttccc gctttcctcg aacccgctga tgaaggcatc
     1201 cttgagggcg cgcgccaact ctccgctgcc gaggttttct gccgcactgg cccgctgtgc
     1261 aatggtcgtc gcgaaggccc atctctccca attggtcccc gtgttcaggt aacgaatgat
     1321 cgcgtctgtg acctggccca ttcggtagat gcggccttct tgctgcatgg ccatcgtcgg
     1381 ctgcgtcggc tgcccgagat tgaacagaac gcgctgatgc ttgccggtgg tgtcgtgcag
     1441 gctgatgcct tctttcccgg cctgcgactg caccaggatc agttgcggtc cgctggcgtc
     1501 gtcctgaaac ctggccacgt tttcgcgacg cgtcttggcc ggcaccaggc cattgaccaa
     1561 caacacgcca gggaattgct tctgaaagtt ggcgataggc gaagaagcct tgaacagatc
     1621 cgaaccgatc aggtccttga attccgtctt gaactctctg atgaccgagt tgatgagatc
     1681 ggtatcgccg ctttccgaaa actcggctcc cctctcgcgc tcgttcatgt cgaacggatt
     1741 gaacccgccg ccttggttgt agtcatggaa gacgaccacc ttgcggccca tcgccatgtg
     1801 ctctcgaatg tgtgggataa cctcctgtgc cttgatggct tcgagcagat agcgcctcga
     1861 ctggtaatca aacttgtcgg caatgacctt gcgaacatct ctcagcgcgg cttttcggtc
     1921 cttgtcgttg gtctttttcc cctgatcctc gaaccactca agcgcctcgt cgatgcgctg
     1981 gccgatggcc gactctacaa ggaggaaacg ccggtcatag tcggcctcga catccaggac
     2041 tctcccggac agcgacccct tcttcttcaa gaaggcgttg aattgtctct gcatcagccc
     2101 gcggtcgacc ttggcatctg gctgcgtcaa cttgccggta cgcatccggt agccaaagtg
     2161 ctgcatgaag aactggtcgc tgttgctgcc ctggttgtag cccctggact ccgtcttgtc
     2221 tgatggcctg ccctcgttgt aatcgaacag gtagccgttc gcccaatcga tcgacggttc
     2281 gtaggcgaag ggggtgaagc tcaggaacaa tgcgcgcggg cggacggcgc cctgtctgga
     2341 ttccacatcc tcgcgaacaa tcttctgcgt ctcgcggttg cgtttttccg ccagagcgag
     2401 tgactctgaa aggcgggcgt tctcagcggc gatccgttcc ttttcggcga cgggaatgga
     2461 actgctcttg ccaatctcga tgttgttctt gagcctcgca cgaagatcgg ccagcttcat
     2521 gatctcgtcg gcgtacagcg ccgcgtgccg gtcgttcgcc ccgtccggat gaagcgtgat
     2581 ggcgcgtagc gtgcgcaggg cattggtcga tcgcgcctcg ctgttttcca tcaagtggtg
     2641 cgcttcatcg tgcacaacga gatcccattc gcgcctgacc agttccgagt tggcccccat
     2701 gttggcgtaa gtcgtgacaa caatcccctt gccggcatcc ttggtgttct tcagctcgga
     2761 aatatccagg cccaacatcc tcccagactt ctgccagtcc tcgatgattt tgtcgtttgg
     2821 tacgacgatc aggatgttgt tcttgccggc gtttgaaaat cgcttgatga tgccgagtcc
     2881 ggaaaacgtc ttgccggtcc cggtcccgtt cgtgaagagc atcccgtacc catcaggaac
     2941 ggcgaaccgg cgttctgcct tggctacgtc ctcttgctgt ccggcagaca gcatcggcag
     3001 cgcgtcggcg atcgatgcgc cggggtcggt tggcgcgctc gcctgcagcg cgatctttct
     3061 ggctaggtca ggtcggcctg tagcagccgc gccaatacct tttgctgcat cgggctcaat
     3121 gggcggtcca tcgaggcgta gatcaccgcc tcgctgactg acgctatctc cggaagcgcc
     3181 tggagaaact ccgggtgctc caacgtaaac ctcgttatcg ctcgccgctc gatcagcaag
     3241 ggcttcaggt ccaggaatgc cgacgccact accggagcta cccgtcgttt cagcgcctgg
     3301 tactcccggt cctccagctc cgccatctcg gcctgatcca tcgcgaacgc ccttcttgcc
     3361 cactccgttt tcagtggctg cgttctcgct atctcgttcc agatcggtgc cgctacgctc
     3421 gtcagggaca tttccttgct ccagttcggt ttttgactcg acgctcacca cgtcgcgctt
     3481 tgcggtggcc aattccttgc cgccggacat ggcgatgtag gcgccctgca gatgatcaat
     3541 cgtgaccgca tcggccacct ccgccccgat cttctcgcga atggtctgca agacgaagcg
     3601 ggcagcatcc ttgaacgaat gatagcccaa ccggaaagcc gcatcgaaca cgcgcaccat
     3661 gaccgggaac agtttttgtt cctgttccgg catcatgttg gcgcggaact tcttgccgaa
     3721 gatatcggcg aggtcgctga gcgcgtcgtt caggtctttg gcggcttgct gtttttctgc
     3781 tgacggtgat tcttcgctgt tggcgctttt tcccttatcc gccattctgc tggcatcggc
     3841 cagcgcagaa aatgcttcgt cgcgcgtatc aaacccgtct ttgctttcga cgcctccaag
     3901 gttagagaca aaaaaccatt tccccgtcgc gatgttcttg tcgacgattc ctccggtcgt
     3961 cttgtcacga ctggccgcca tccctccggg ggatgcttct gtccatcctg ccggcagtgc
     4021 gtcttcggga gattccttgc gggccttggt cgcatcaata gcgtccgcca gatcatacaa
     4081 ctccctggcc ttgcggacct t
//
LOCUS       DAOQEW010000286         4092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9613_length_4092_cov_8.465692, whole genome shotgun sequence.
ACCESSION   DAOQEW010000286 DAOQEW010000000
VERSION     DAOQEW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9613_length_4092_cov_8.465692"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLS35_18640"
     CDS             <1..773
                     /locus_tag="PLS35_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20745.1"
                     /translation="SNLPVDGSKPMTVFSDEDVRYLAPLLAQALSQAQPEYVVAFRLS
                     SSAGSGSEPTAGTLYVKNDRLYLTLSEHHGSLAKTESTWLGSGRAARIVSVMPASAGQ
                     LEQALPAVIQGRQQMKSLAIDYHQFAKKPEPAPMTAATTAPAPQPVQALSVRNVPAVA
                     PDQPAKQQSAPAPVMDKVASNQAPAEDERLNETLQELQDTKDAMARKDAKISALRRDL
                     ESMRMQLETKDKELRAVKNTQQQQPKRETRNKAELTVR"
     gene            complement(789..1505)
                     /locus_tag="PLS35_18645"
     CDS             complement(789..1505)
                     /locus_tag="PLS35_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNI20746.1"
                     /translation="MKVVLDIETVQAPREEWARIAGRQLTSGEVTFAESGGDLFAVGE
                     AEAQQRLDDELYEKSSFDGTFSKIVCIGLLEFTDTLEPRGAISWYGGNEQALLRAFWS
                     HLGQSRPSLFITHNGLNFDLPFIKKRSIIQQVKPSMEINLAKFRAEPVYDTMAIWSNW
                     DNRGWVKLDVLARALNVESKSGSGSQVAQMWADGQGKEIALYCLQDTYVTYGCYCRMN
                     FRPPISREIVLLKPELVDVG"
     gene            complement(1512..1961)
                     /gene="mutT"
                     /locus_tag="PLS35_18650"
     CDS             complement(1512..1961)
                     /gene="mutT"
                     /locus_tag="PLS35_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNI20747.1"
                     /translation="MIVKCEGLMGQRLDTGKVAMQVIEVAAGIIAHEGRYLIARRKAG
                     AHLGGLWEFPGGKRETGETLEECLHRELWEELQVRVSALTPFQIVRHEYPEKVVVLHF
                     FRCRIEAGVAIAIDCAELRWVYPHEMASFEFPPADQPVIAALQQRKV"
     gene            complement(1984..2703)
                     /locus_tag="PLS35_18655"
     CDS             complement(1984..2703)
                     /locus_tag="PLS35_18655"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNI20748.1"
                     /translation="MPTKPRTQKPSRRKKQTSTPALPLARGQKQRFQNRLLKWYKEYG
                     RDLPWRKTSDPYHILVSEVMLQQTQVDRVIPKYHEFLERYPSFAELADAPVAEVKQTW
                     YPLGYNIRPERLHSIARETVARYGGQLPSDADELLSFKGIGRYTAGAIRSFAFNEDAP
                     ILDTNVIRVLHRVFIAEGDPKGQKALLWELSEALIPRGKGYDFNQAIMDFGATICTAR
                     NPYCLLCPMKPLCKTYPFGEK"
     gene            complement(2855..3220)
                     /locus_tag="PLS35_18660"
     CDS             complement(2855..3220)
                     /locus_tag="PLS35_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20749.1"
                     /translation="MKASFSIYALAAIGMLGAAVAFANPALLPQHPGYPMGKATDPVN
                     GQALANDPGRANGGGESALAGSAAFDDRHVSQKLSINDQNQRLLEKPGAGILPKVQGP
                     NIVIDPPVKEGTKVQASPQ"
     gene            3778..>4092
                     /locus_tag="PLS35_18665"
     CDS             3778..>4092
                     /locus_tag="PLS35_18665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNI20750.1"
                     /translation="MSMTVHYRVSLSADSTTTGEGRLLDLSLEGCRIECDRHLPVNTY
                     LSLRLQISPNEMPILVDLAAVRWTRGTNCGVHFLSVQPLQSARLQTFLAAASPRKEPP
                     TPG"
BASE COUNT          881 a         1274 c         1111 g          826 t
ORIGIN      
        1 tgtcgaatct gcccgtggat ggcagcaagc cgatgacagt gttcagcgat gaggatgtgc
       61 gttatcttgc tcccctgcta gcccaagcgt tgtcccaagc gcaacccgaa tacgtggtcg
      121 ctttccggct ctcctcatcg gcagggtcag gctctgaacc caccgcgggc accctttacg
      181 tgaagaacga ccggttgtat ctcacccttt ccgagcacca cggctcgctg gccaaaaccg
      241 agtcgacatg gctgggaagc gggcgagcgg ctcgaatcgt gagcgtcatg ccggcctccg
      301 ccgggcagct cgaacaagcg ttgccggccg tgatccaagg acggcagcag atgaagtcgc
      361 ttgcgatcga ctatcaccag tttgccaaaa agcccgaacc ggctcccatg accgcggcga
      421 ccactgctcc ggctccccaa cctgtccaag ccctcagcgt gcgaaacgtc cctgccgtgg
      481 cacccgatca accggccaag cagcaatccg cgccggcgcc agtcatggac aaggtggcgt
      541 cgaaccaggc ccctgccgaa gatgaacgtt tgaacgagac gcttcaagaa ttgcaggaca
      601 cgaaggacgc catggcccgt aaggacgcca agatcagcgc gctgcgaaga gatttagagt
      661 cgatgcggat gcagttggaa acgaaggata aggaactccg ggcagtgaaa aacactcagc
      721 agcagcaacc caaacgcgag acccgcaata aggcagaact gacggtccgc tgacctcgcg
      781 gaccggattc agcccacatc gaccagctcc ggcttcagca gcacaatctc cctcgatatc
      841 ggcggccgga aattcatccg gcagtagcat ccataggtca cataggtatc ctgcaggcaa
      901 tagagggcaa tctcttttcc ctgcccgtcc gcccacattt gcgcgacctg ggaaccactc
      961 cccgatttgc tttctacatt caacgcccgg gcaagcacat ccagcttgac ccatccccgg
     1021 ttatcccagt tgctccaaat ggccatggtg tcgtagaccg gctccgcgcg gaacttggcc
     1081 aggttgatct ccatactggg cttcacttgt tgaatgatcg accgtttttt gatgaagggc
     1141 agatcgaaat tgagtccgtt gtgggtgatg aacaacgacg gacgcgactg gcccagatgg
     1201 ctccagaagg cccgcaggag tgcctgctca ttccccccat accacgagat ggcacccctc
     1261 ggttccaggg tatccgtgaa ctccaacaga ccgatgcaga cgatcttgct gaacgtgccg
     1321 tcgaaggaag acttctcata cagttcatca tcgagccgct gctgagcctc tgcctcaccc
     1381 acggcaaata gatcaccgcc gctctcagcg aaagtgacct cgcccgacgt gagctgccgc
     1441 cctgcgatcc gcgcccattc ttctctggga gcctgcacgg tttcgatatc cagcaccact
     1501 ttcatacgcc cctacacctt cctctgctga agcgccgcaa tgacgggctg gtcggccggc
     1561 ggaaactcga aggaggccat ctcatgagga tagacccatc gaagctccgc gcaatcgatc
     1621 gcgatcgcaa cacccgcctc gatgcggcaa cgaaagaaat gcaataccac aaccttctcg
     1681 ggatattcat gccgtacgat ctgaaaggga gtcagtgcgc tgactcgaac ctgcaactcc
     1741 tcccataact cccgatgcag acattcctcc agcgtttcgc ctgtttcacg tttgcccccg
     1801 ggaaactccc acaaccctcc aagatgcgcg ccggctttgc ggcgggcgat caggtagcgt
     1861 ccttcgtgag caatgattcc ggccgctact tcaatcacct gcatcgcgac cttcccggta
     1921 tcgagtcgtt gccccatcag cccttcgcac ttgacgatca tgcgatgacc cgttcgtcga
     1981 ggattatttc tcaccgaacg gataagtttt gcacagcggt ttcatgggac agaggaggca
     2041 atagggatta cgcgccgtac aaatggtggc gccgaaatcc atgatcgcct ggttgaaatc
     2101 gtacccctta ccccgaggaa tcaacgcttc ggagagttcc cagagcaacg ctttctgtcc
     2161 cttcggatcc ccctcggcga tgaacacccg gtgcaagaca cggatcacgt tcgtgtcgag
     2221 gatcggcgcg tcctcgttga acgcgaagga gcgaatcgcg ccggccgtat accgcccgat
     2281 gcctttgaag gacaacaatt catccgcatc actcggtagt tgcccgccat agcgggccac
     2341 ggtttcacgc gcgatactgt gtaaccgctc gggacggatg ttgtatccca acgggtacca
     2401 ggtctgtttg acctccgcta ccggggcatc ggccaattcg gcaaaggacg gataccgctc
     2461 caaaaattca tggtacttcg ggatgacccg atccacttgc gtctgctgga gcattacctc
     2521 cgagaccaga atatgatagg gatccgacgt cttccgccag ggcaggtcgc ggccatactc
     2581 tttgtaccac ttgagcaacc ggttctgaaa tcgctgcttc tgtccgcgcg caagcgggag
     2641 ggcgggagtc gacgtctgtt tcttccgtcg ggaaggtttc tgagtgcgag gctttgtagg
     2701 cataacggtc cgtgagacgt ggcggcattc taaaaggcgg tggggtgaaa atcaaaccag
     2761 cgtgaaggag cgggagagga ccgcaggaca gggtctccca atggaatact ccccgtcctg
     2821 cggcgtgtga tgcgacgtta cacaagcgat ggtgttactg cggtgaggcc tgcaccttcg
     2881 ttccctcttt tacgggcgga tcgatcacaa tattgggtcc ctgcaccttc ggcaaaatcc
     2941 cggctccggg cttctcgagc aggcgctggt tttggtcgtt gatcgaaagt ttctgtgaca
     3001 cgtgccggtc atcaaatgcg gcggagccag caagtgcgga ttccccgccg ccgttggcac
     3061 gtccaggatc gttagcgaga gcctggccgt tgaccggatc ggtcgctttg cccatcggat
     3121 acccgggatg ctggggcaac agcgcaggat tggcaaaggc tacggcagct cccaacattc
     3181 caatagccgc caacgcatat atggagaacg aggctttcat acgacacctc ctttggggcg
     3241 gataacatga cagcgccctg cggccgacca gccgcagggc gcacaatgaa cctccggagt
     3301 tgaatggagt cctctcttgc tcttgcggaa caattcaccc cggcatggca agtatctccc
     3361 gatacctttc cagcttcttc ctccataaga acctagtcaa gagggcgatc atcatccgac
     3421 taagccactc ttccgggcat gattgatcag cacgtagttt gaagaaccac aaacggatgc
     3481 cgattttcag gtcccattcc tcaaatcaag gaccagagcc gatttcctgc cctaggactt
     3541 tccccaccgc caaatgaccc gagacattat cctgatcgca caggcaatgg agggcatggt
     3601 atgtcagtct gccggcccac tcgtcgcccg aggccttcgc tctttcgcat cggcgatgaa
     3661 gcgcgagacg ttgatgcgac gagcggctct gtggactcgt ttcaggcttc acctggagtt
     3721 cgacggtgca ccagggtagc gccattattc actacacccg aggatgcaca gcaatacatg
     3781 tccatgaccg ttcattaccg tgtgtccctg tccgcggatt ccacgacgac tggagaagga
     3841 cgcctgttag atctctccct ggaaggctgc cggatcgaat gcgaccggca tttgccggtc
     3901 aatacctacc tctcgttacg tctgcaaatc tcaccgaatg agatgccgat tctggtggac
     3961 cttgccgcgg tgcgatggac tcgcggtacc aattgcgggg tgcatttctt gtccgtccag
     4021 ccgctgcaat ccgcacggct gcagaccttt ctcgccgccg cgtcgccccg gaaagaacca
     4081 ccgactcccg gg
//
LOCUS       DAOQEW010000287         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9629_length_4088_cov_9.023060, whole genome shotgun sequence.
ACCESSION   DAOQEW010000287 DAOQEW010000000
VERSION     DAOQEW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9629_length_4088_cov_9.023060"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PLS35_18670"
     CDS             complement(<1..870)
                     /locus_tag="PLS35_18670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20751.1"
                     /translation="MRKMPHVCILTSQYFDWGIYGGFGSMSRKLAESLVRAGHRVSVI
                     VPGRQGQQPTETIGGVEIRSFSPRNVMDACRLIRESRADIFHSQDPTVLTYLAQRIHP
                     RRAHLVTSRDPRELSDWWVEFLYATPMRRLLTPLNYFTESGFLVRQAVRRADAVYCPA
                     HFLKEKVKRLYGLKQLPTLLPNLIDVPPALPRKSDRPTITFVARWDKRKRPWLFLELA
                     AQFPDYRFVAVGKGSASAETGFDAELRQRFRNVPNLEMPGLINRFREPERMHQLLSDT
                     WIFVSTAVREGLPL"
     gene            complement(867..2078)
                     /locus_tag="PLS35_18675"
     CDS             complement(867..2078)
                     /locus_tag="PLS35_18675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI20752.1"
                     /translation="MFDNEFPPLGGGTGVVNLHLLEEMALRPDVTVDLVTSSRTKARY
                     ETEAFADRITIYKVPVDNRNIHHATNRELLRYSWRGLRLARQLASEHQYDVSFAFAGV
                     PAGAISYLLRLTHGLPYVLSLQGPDVPGFEARYNYLYPVLTPFITQIWRRAGVVTAIS
                     AEQVALAHRTMPDLPLVTIPNGVDTNAFVPATRNSGRPFTMICVARLIERKGQHHLLQ
                     AFANLRAANHEPMALIFVGTGDAEPQLRELAAKLQVAEAVTFKGFVPRENMPPVYHDA
                     DVFVLPSQHEGMSIALLEAMASGLPVIVTDTGGTAELVTQGANGEIVPWDDVEALTTA
                     LQRLLNDAEQRRRMGDESRRRALAFGWPTLAARYLELCARVMVPSAESRAKDARSAKN
                     VARDWTRELRG"
     gene            complement(2096..>2794)
                     /locus_tag="PLS35_18680"
     CDS             complement(2096..>2794)
                     /locus_tag="PLS35_18680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20753.1"
                     /translation="AISAGLEHCRGRVVSIMDADLQDPPEVLHTFLAKWQEGWEVVYA
                     VRTERKEWWGKRLAYAGFYRLLQRVANIEIPLDAGDFCVMDRKVVDLLVRMPERNRFV
                     RGIRSWVGFKQVGVPYERQARHAGTPKYTLRKLIYLALDGLVSFSHMPLRIITLLGFT
                     VSLLSFLVALFYLIKKFTIGVGVAGFTTLVVSIFFLAGIQLMTIGVIGEYIGRISDEV
                     KHRPLYVARRVTRR"
     assembly_gap    2795..2894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3050..4045)
                     /locus_tag="PLS35_18685"
     CDS             complement(3050..4045)
                     /locus_tag="PLS35_18685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNI20754.1"
                     /translation="MLKAALLLLGALTLSALVWHIGIGRIYDAVTQLGPAAMVLVLLP
                     SLLMYVLEAYGWRVTLGAWAKGVPFWRILAIRTAGEVVNMTTPTAYVGGEPLKAYLLN
                     RHGVPLVEGLASVVTAKTTMTIAQILFILAGIGLGFWLLGSGGSAGQTITAGLVSVGL
                     LLFGVGAFVIVQRQGMFGWILALLRRIGLRIQFLEAREQQLLELDRTIAGFYSHQRQA
                     FLLSTGLFLLGWLAEALEVYVMVLCMGQSINVLPAVAIGALSVFIKGGTFFIPGSLGA
                     QDAGNLFLLSAFGYGEVTGITFALLRRFREFVWIALGLGCLAMMTKGADPSPSQK"
BASE COUNT          900 a         1218 c         1141 g          729 t
ORIGIN      
        1 caatggtagg ccttcgcgta ccgccgtgct gacaaaaatc caggtatccg agagcagttg
       61 atgcatccgt tccggttcac gaaaccgatt gatcaggccc ggcatttcaa ggttgggcac
      121 gttgcggaat cgctggcgta actcggcatc aaaaccggtt tcggcggaag cgctcccttt
      181 gcccaccgcc acgaaccgat agtcggggaa ctgcgcggcc aattccagaa acagccaggg
      241 gcgcttgcgt ttgtcccaac gggcgacaaa ggtgatcgtc ggtcggtcgc tttttcgagg
      301 gagcgccggg ggcacgtcaa tgagattggg gagcagcgtc ggcaactgct tgagcccata
      361 cagacgcttg accttctcct tgagaaaatg ggccggacaa tacaccgcat cggcccgccg
      421 caccgcttgc ctgacgagaa accctgattc tgtgaaatag ttcaggggcg tcagcagacg
      481 gcgcatcgga gtggcataca gaaactccac ccaccagtcg ctcagttccc gcggatcccg
      541 gctcgtcacc agatgcgcgc ggcgcgggtg aatgcgttgc gccaggtagg tcaggaccgt
      601 aggatcctgg gaatgaaaaa tgtccgcgcg ggattcgcga atgaggcgac aggcatccat
      661 gacattgcgc ggcgagaagc tgcgaatctc caccccgccg atggtttctg tgggctgttg
      721 gccctgacga ccgggcacga tcacgctgac ccgatggccg gcacgcacca ggctttcggc
      781 cagcttccgc gacatgctgc cgaagcctcc ataaattccc caatcaaaat actgactggt
      841 cagaatgcac acatgaggca ttttcctcat ccccggagct ccctggtcca atcgcgagcc
      901 acgtttttcg cacttcgggc atccttggct cggctttcgg cggacggaac cataaccctc
      961 gcgcacaact caaggtagcg ggcggcgagc gtgggccatc cgaatgcgag cgcacgacga
     1021 cgactttcgt cgcccatgcg ccggcgctgc tcagcatcgt tcagcagccg ctgcaacgcg
     1081 gtcgtgagtg cctcaacatc atcccatggc acgatctcac cgttggctcc ctgcgtcacc
     1141 aattccgcag tgcctcccgt atcggtcacg atcaccggga gcccggaggc catcgcttcc
     1201 aagagggcga tcgacatgcc ttcatgctga gacggcaaca cgaacacatc ggcgtcgtga
     1261 tagacaggag gcatgttctc gcgcggcaca aagcccttga acgtcaccgc ctccgccact
     1321 tgtaacttcg ccgccaattc tcgcaactgc ggctcggcat ccccggtccc gacgaagatc
     1381 agtgccatcg gttcatggtt tgctgctcgc aagttggcga aggcctgtag cagatgatgt
     1441 tgccctttgc gttcaatcaa tcgagcgaca caaatcatgg tgaacggtcg tccagaattc
     1501 ctcgtggccg ggacaaacgc gttcgtatca accccgttgg gaatcgtaac gaggggcaga
     1561 tccggcatcg tgcgatgcgc caacgccacc tgttctgcgc tgatggctgt caccacaccc
     1621 gctcgacgcc agatctgtgt gatgaacggc gtcaacaccg ggtagaggta gttgtatcgc
     1681 gcctcgaacc ccggcacatc gggcccctgt aacgacaaga cgtagggcag tccatgcgtg
     1741 agacgcaaca ggtaactgat ggcaccggcc ggcacacctg caaaggcgaa gctcacatca
     1801 tactgatgtt cggacgctag ttgccgtgcg agacgcaatc cccgccaact gtaccgcagc
     1861 aattcacgat tggtggcgtg gtggatgttg cgattatcga ccggcacctt gtagatcgta
     1921 atgcgatcgg caaaggcttc ggtttcgtat cgcgccttgg ttcgcgagga cgtcacgagg
     1981 tccaccgtga catcgggccg caaggccatc tcctccagca aatggagatt gacgacgccg
     2041 gtgccgcccc cgaggggagg gaactcgtta tcgaacatga gaatgcgaat ggtgattatc
     2101 tcctggtcac acggcgggcg acgtacagcg gccggtgttt cacttcatcg gatatgcgtc
     2161 cgatgtattc cccgatgacg ccgatcgtca tgagctgtat ccccgcgagg aagaaaattg
     2221 agacgaccag cgtggtgaac ccggccaccc cgaccccgat ggtgaatttc ttgatgagat
     2281 aaaacagcgc cacgaggaag gacagcagcg aaacggtaaa gcccagcaac gtgatgatgc
     2341 gcaacggcat gtggctgaat gagaccaacc cgtcgagcgc cagatagatc agcttccgaa
     2401 gcgtgtactt gggcgtcccg gcatggcgcg cctggcgctc atacggcacg ccgacctgtt
     2461 tgaatccgac ccagctgcgg atgcccctga caaatcgatt ccgttcaggc atgcgcacga
     2521 gcaggtcgac gactttgcgg tccatgacgc aaaaatctcc ggcatccaac ggaatctcga
     2581 tgttggcgac ccgttgcagc aagcggtaga atcccgcgta ggctaagcgt tttccccacc
     2641 attccttccg ctcggttcgc accgcataga cgacttccca cccttcctgc cacttggcta
     2701 aaaaggtgtg aaggacttcg ggaggatcct ggagatcggc atccataata ctgacgacac
     2761 gtccgcggca atgctccagt ccggcgctga ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnaaaacc gttacctggg attggatatc tcagggttgc tccctagtgt
     2941 agccgaggag gtccaattcg gccacatctt ccactgaaat tgcgctgtag gaaattcggc
     3001 atggctggtc tgtctcagcc tggcacaagt cccggtgtgg aacggaggat tatttttgtg
     3061 aaggggaagg atcggcacct ttcgtcatca tcgccaggca gcccagccct aaggcaatcc
     3121 acacgaattc ccgaaagcgc cgcagaagcg caaacgtaat acctgtgact tcgccgtacc
     3181 cgaacgcgga gaggaggaac agattgcccg catcctgtgc ccccaaactg cctggaataa
     3241 aaaacgtccc tcctttaatg aagacggata aggcaccaat ggcgacagcc ggcaacacgt
     3301 taatcgattg ccccatgcac agcaccatga cgtagacttc cagtgcctcc gcgagccaac
     3361 cgagcagaaa cagtccggtc gaaaggagaa aggcctgtcg ttggtgggaa taaaagccgg
     3421 cgatggtgcg atccagttcg agcaattgct gctcacgcgc ctctaaaaac tggatgcgca
     3481 gaccgatgcg tcgcaacagc gccaggatcc acccgaacat accctgacgc tgcacgatga
     3541 caaaggcgcc aacaccgaag agcagcaggc ccacgctgac caatcccgcg gtgatcgtct
     3601 gtccggccga tccgccggaa cccaatagcc agaatcccag gccgatgccc gcaagaatga
     3661 acagaatctg ggcgatggtc atggtcgttt ttgccgtcac gaccgacgcg aggccctcta
     3721 cgagtggcac gccgtggcga ttgaggaggt aggccttcag cggttcgccc cccacatagg
     3781 ccgtaggcgt agtcatgttc acgacttcgc cggcggtacg aatcgctaag atgcgccaaa
     3841 acggcacacc cttcgcccag gcgccgagcg tcacgcgcca accataggct tcgagcacat
     3901 acatgaggag agagggaaga aggaccagca ccatggcggc aggaccgagt tgcgtcaccg
     3961 catcgtaaat acgcccgatg ccgatatgcc aaaccagtgc cgagagtgtc agcgccccga
     4021 ggagaaggag ggccgcttta agcacgggcc cgtgccgagg gtcggatcac ccacaccatc
     4081 atcaacca
//
LOCUS       DAOQEW010000288         4088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9630_length_4088_cov_7.338954, whole genome shotgun sequence.
ACCESSION   DAOQEW010000288 DAOQEW010000000
VERSION     DAOQEW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9630_length_4088_cov_7.338954"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLS35_18690"
     CDS             <1..639
                     /locus_tag="PLS35_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNI20755.1"
                     /translation="PQPKQALTKRLTNALKLAVVPPVGYAVIRLLQRTMGWRTEGREH
                     VSRIFAQGQQAILAFWHAQQLMMPLAIPGLKAHILISQHRDGELICRIVARFGLDAVR
                     GSSTRGGAEAFRRLVRLGRSGGNLVLTPDGPKGPRQVAKVGVVQLARATGLPIVPMAF
                     GCSKKNSLRAGTGSSCPIHSRRGSSSSGHRLQSRRMHPLKTWSACAGSWKRP"
     gene            689..2044
                     /locus_tag="PLS35_18695"
     CDS             689..2044
                     /locus_tag="PLS35_18695"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HNI20756.1"
                     /translation="MWYLLYNSLLLLVSPVILCVLLAKQRCRRGLPQRLGLLPEQPRE
                     AALAPTCIWIHAVSLGEVVAVAPLVRELRRRYPEARLVVSTVTETGREAVEQRLEGLA
                     EHRYAPLDFPWVVNQTIDLLRPNLYVFVETELWPNLLRSLRRRNIPSILVNGRLSTRS
                     FERQRLPLIRDFYRTMLNMISCCLMQSERDAQRMVELGADQSRVRCTGNIKFDQPVPQ
                     AAAGGTVVSKQALGLSERERLVVAGSTHPGEEDMIVDAYQSVCEEFPELRLVLAPRHI
                     ERAAQVEQMVRAKGLPVSRRSTGKMELMATSGPRVVVLDTRGELALLYREAEVAFVGG
                     TLVPIGGHNLLEPAVWGKPVLFGPHTDHCAEVATLLLNARGGRVVDGAQGLAQSLREL
                     LGNSDDLKRMGQAAQQVVADNQGALQRSAEIIATFLPPSATSTRSEGATRGALPHPRS
                     S"
     gene            2050..3108
                     /gene="lpxK"
                     /locus_tag="PLS35_18700"
     CDS             2050..3108
                     /gene="lpxK"
                     /locus_tag="PLS35_18700"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNI20757.1"
                     /translation="MRQSWLRWVGYPYEVAARLRLWGYARGWFQTHRLPRPVISIGNL
                     TMGGTGKTPVVMYVVDRLIAQGKRVAILSRGYRRRSKAPQLLVSDGQQVLVGPEEAGD
                     EPYLMARRCPHAVVAVGADRYALGQWVLSRLPVDCFVLDDGFQHVQLHRDVDLLLVDA
                     TDTAGLRAALPVGRLREPLSAAARASAVLITRVDREEAGEQVWRQLVQACPALPSPVC
                     VGFPAEEFRRIGGDEGRPRDAFRGRSAVIFSGIGNAGSFHAVVAELGITVIETLAFPD
                     HVHYTHAMMETIRARAQAGGVDLLVTTEKDADKVAPFLNADDACWAIRLRTQIISGQE
                     RLERLLRLEPESRPASDA"
     gene            3074..>4088
                     /gene="waaF"
                     /locus_tag="PLS35_18705"
     CDS             3074..>4088
                     /gene="waaF"
                     /locus_tag="PLS35_18705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNI20758.1"
                     /translation="MSRKVGRRAMRKEEIRRIVVRGPNWLGDAVMCEPALSQVRTLFP
                     QAEITLLVKPAIADLLAQHPAVNRTLVYDDRGRHAGLTGKWTLAGVLRRHRFNLAILF
                     QNAFEAALISFLAGIPRRFGYATDGRSVLLTDPVAVPPRSAQRHQVEYYWDLLKPLGG
                     QGQSPAPRLFVTPEESALIARRLADAGIGPSDLVIGVNPGSTYGQAKRWLPERYAEVV
                     NRVVKDAQSQSGARIGVAIVGAKGEEPLGKAIADQIQTRTVVCSGQTTVRELMALVKR
                     CQLFLTNDTGPMHVAAAFNVPLVAVFGPTDWQTTSPFGVEAQLVRQPVSCAPCLLREC
                     PIDH"
BASE COUNT          704 a         1182 c         1376 g          826 t
ORIGIN      
        1 ccgcagccga aacaggccct gacgaagcgt ctcaccaatg cactcaagct cgcggtggtg
       61 ccgcctgtcg gctatgctgt gattcggctc ctgcaacgca cgatgggctg gaggacagag
      121 ggcagggaac atgtgtcccg gatattcgcg cagggccagc aggcgattct cgcgttttgg
      181 catgcgcagc agctgatgat gccgctggcc attcccggtc tcaaagcgca tatcctgatc
      241 agccagcacc gggacggtga attgatctgc cggatcgtgg cgcgtttcgg attggatgcg
      301 gtgcgtggtt ccagtaccag aggtggcgcg gaagcctttc gccggctggt tcgcctcgga
      361 cggtcgggcg gcaacctagt gctgacgccg gacggcccga aggggccgag gcaggtcgcc
      421 aaggtgggcg tggtgcagtt ggcgagggcg acaggtctac ccattgtccc catggccttc
      481 ggttgctcaa aaaaaaactc tttgcgagct gggaccggtt catcatgccc tatccattct
      541 cgcaggggat cttcctcatc ggggcaccga ttgcagtccc gccggatgca tccgctgaag
      601 acctggagcg cctgcgcggg gagttggaag cgaccctgaa ccgcatgacg atcgaggccg
      661 atgcggcggc ccggcagggt cgtccgtaat gtggtatctg ctctacaaca gcctgcttct
      721 cctcgtttct ccggtcatcc tctgtgtgtt gctggcgaag cagcgctgcc ggcgcgggct
      781 accccagcgg ctgggcctgt tgccggagca gccgcgcgag gctgcgctgg cgccaacctg
      841 catctggatt catgcggtgt ctttggggga agtggtggcg gtggcgccgc tggtacggga
      901 gcttcgccgg cggtatcccg aggcgagatt ggtagtctcg accgtgacgg aaacggggcg
      961 tgaggccgtc gagcaacgtt tggaaggtct ggccgaacac cggtatgctc cgctcgattt
     1021 tccctgggtg gtcaaccaga cgatcgatct actcaggccc aatctctacg ttttcgtcga
     1081 aaccgagctc tggcccaatc tcttgcggag cctgcggcgg cggaatattc cctcgattct
     1141 ggtcaacgga cggctctcca cccgatcgtt tgagcggcag cgcttgcccc tgatccggga
     1201 tttttatcgc acgatgttga atatgatcag ctgttgcctc atgcaatcgg agcgtgatgc
     1261 gcaacggatg gttgagttgg gtgccgacca atcccgggtc cgttgcaccg gcaatatcaa
     1321 attcgaccag ccggttccac aggcggccgc cggcggcacg gtcgtgtcga agcaggccct
     1381 gggactgagc gagcgcgagc ggctggtggt ggcgggaagc acgcatcccg gtgaggaaga
     1441 catgatcgtg gatgcctatc agtctgtatg cgaagagttc cctgagctcc gcctggtgct
     1501 ggctccgcgg catatcgaac gggcagcgca ggtcgagcag atggtgcgcg ccaaggggct
     1561 gccggtgtct cgacgcagta ccggcaagat ggaattgatg gccacatccg gtccgcgcgt
     1621 ggtggtcttg gacacgcgcg gagaattagc gttgttgtat cgggaggccg aggtggcgtt
     1681 tgtcggtggc accctggtgc ccataggcgg gcacaacctg ttggaaccgg ctgtctgggg
     1741 aaaaccggtg ctgttcggac cacataccga ccattgcgcc gaagtggcga cgctgttgct
     1801 gaatgcccgg ggcggccgtg tcgtggatgg tgctcaaggc cttgcacaga gcctgcggga
     1861 gctcctcggc aattctgacg atctgaaacg gatgggacaa gctgcgcagc aagtggtcgc
     1921 ggacaaccag ggcgcgcttc agcgcagcgc ggagatcatt gccacctttc ttccaccatc
     1981 cgccacatcg acccgctccg aaggcgctac gcgcggagcc ttgccgcatc cacgatcctc
     2041 atgaggccga tgcgacaatc ctggttgcgg tgggtgggat atccgtatga ggtagcggcc
     2101 agacttcgcc tgtggggcta tgcacgcggg tggtttcaga cacatcggtt gcctcgcccc
     2161 gtgatcagca tcggaaacct cactatgggc ggaaccggga aaacgcccgt cgtcatgtac
     2221 gtcgtcgacc ggttgatcgc ccaaggcaaa cgcgtcgcca tcctgagccg cggctaccgg
     2281 cggcgcagca aggctccaca attgctggtg tcggacgggc agcaggtgtt ggtgggaccg
     2341 gaagaggccg gtgatgaacc ctatctcatg gcccgccgtt gtcctcatgc ggtcgtggcg
     2401 gtcggggcag accggtatgc ccttggccag tgggtcctga gccgtttgcc ggtggattgt
     2461 tttgtgctcg atgatggatt tcaacatgtg caactccacc gcgatgtgga tctcttgttg
     2521 gtggacgcca ccgatacggc tgggctcaga gcagccttgc cggtcggtcg cttgcgggaa
     2581 cctctttcag cagcggcccg tgcatcggct gtgctcatca ccagggtcga tcgtgaagag
     2641 gcaggtgaac aggtctggcg gcagctggtg caggcatgcc cggcgcttcc gtcccccgtc
     2701 tgtgtggggt ttcccgcaga ggaattccgg cgcataggtg gggatgaagg ccggccgcgg
     2761 gatgcctttc gcggccgctc ggcagtcatt ttcagcggga tcggcaatgc cgggtctttt
     2821 cacgcagtag tggcggagtt gggtatcacg gtgattgaga cactggcatt tcccgatcat
     2881 gtgcactata cccatgccat gatggagacg attcgtgcca gagcacaagc ggggggcgtt
     2941 gacctgctgg tgaccacgga gaaagacgca gacaaggtgg cgcccttcct gaacgctgat
     3001 gacgcctgtt gggccatccg gcttcgcacg caaattattt ccggacagga acggctggag
     3061 cgactcctgc gccttgagcc ggaaagtagg ccggcgagcg atgcgtaagg aggagatccg
     3121 tcgcatcgtc gtgcgcggtc cgaattggct gggcgacgct gtcatgtgcg agcccgccct
     3181 cagtcaggtg cgcacgttgt ttcctcaggc ggagatcacg ctgctggtca agccggccat
     3241 cgcggatctg ctggcgcagc atccggcggt gaatcgcaca ttggtctatg acgaccgggg
     3301 acgtcacgcg ggattgaccg gcaagtggac cttggccggt gtcttgcgcc gccatcgatt
     3361 caatcttgcc attctgtttc agaatgcgtt tgaggcggcg ctgatcagct tcctggccgg
     3421 cattccccgc cgattcgggt atgccaccga tggccggagt gtgttgctca ccgatcccgt
     3481 cgccgtgccg ccgcgtagcg cgcagagaca ccaagtggaa tattattggg acctgctcaa
     3541 acccttgggg ggacaaggcc agtctccggc gccgcgactg ttcgtcacgc cggaagaatc
     3601 cgcattgatt gcccgccggc tggctgatgc gggaatcggt ccgtccgatc tggtgatcgg
     3661 cgtgaatccc ggttcgacct acggccaggc caaacgatgg ttgccggagc ggtatgccga
     3721 ggtcgtcaat cgtgtcgtga aggatgcgca gagtcagtcg ggggctcgga tcggagtcgc
     3781 catcgtggga gccaagggcg aagaacctct ggggaaagcc attgccgacc agattcagac
     3841 tcgcaccgtc gtgtgttccg gccaaaccac agtgagggaa ttgatggcgc tggtgaagcg
     3901 ttgccagttg tttctgacga acgataccgg ccccatgcat gtggctgctg ccttcaacgt
     3961 gccgctcgtg gcggtgttcg gtcccactga ttggcaaacg acgtcaccgt tcggcgtcga
     4021 ggcccagctg gtccggcagc cggtatcttg cgcaccatgc ctgttgcggg aatgtcccat
     4081 cgatcatc
//
LOCUS       DAOQEW010000289         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9659_length_4080_cov_7.579876, whole genome shotgun sequence.
ACCESSION   DAOQEW010000289 DAOQEW010000000
VERSION     DAOQEW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_4080_cov_7.579876"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..654
                     /locus_tag="PLS35_18710"
     CDS             352..654
                     /locus_tag="PLS35_18710"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNI20759.1"
                     /translation="MHTPCDLSLLIDGLSSTVTRADIVALFSSCGKVTAAEIVIDADG
                     MPLGVARVTMSTKEEARFAIRTFHRCRFRGHTLLVFEDTTKNQREDLWNSRMFKRP"
     gene            complement(889..1518)
                     /locus_tag="PLS35_18715"
     CDS             complement(889..1518)
                     /locus_tag="PLS35_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20760.1"
                     /translation="MLRILIGDDHAVFRRGTKDVLQEHFAPLEVHEAKSGWEMVTLAS
                     QGKWDAFIMDVTMPGKSGTDLLEHIRSLHPSTPVLVYSMHSEESYAVRMIRSGASGYL
                     NKSTGLDELVAAMRSILAGQEFVNPAVARCLIERMRTKDSGQLHDALSNREFQILRMV
                     AQGESLKQIALTLCVSANTVSTYRTRILQKLRLKTNAELTRYALEHALI"
     gene            1924..2238
                     /locus_tag="PLS35_18720"
     CDS             1924..2238
                     /locus_tag="PLS35_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20761.1"
                     /translation="MMETVHIVPNEQPTYEESLRLGWALLWRGVGSFVLLMFAGNIGL
                     LFLIPELTRTVPTLWIALLPLAFATLISAFLIMPFVVRRLVKAPFKGFHLSFVRDRLL
                     GQ"
     gene            2475..2975
                     /locus_tag="PLS35_18725"
     CDS             2475..2975
                     /locus_tag="PLS35_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNI20762.1"
                     /translation="MLWLFTTHGSVGMFIVRVTLAIVIFAHGAQKLLGWFGGQGFTGT
                     IGFFTTSMGLPWLIAFLVIIGESIGSLSLLAGFLTRFVAASYLLIMLGAVAMVHWPNG
                     FFMNWFGQQQGEGFEYHLLIIGMSVALLLEGGGAWSVDHLIARWMAQHGWAGTQPLHA
                     FAQSKQ"
     gene            complement(3323..>4080)
                     /locus_tag="PLS35_18730"
     CDS             complement(3323..>4080)
                     /locus_tag="PLS35_18730"
                     /inference="COORDINATES: protein motif:HMM:NF013215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20763.1"
                     /translation="LLPRIRQRIERDLALPGLPREKILATVVRLLETTLIRVGNEEYA
                     KTNNSFGLTTMRDRHARVEGARVRFEFRGKSGVQHLVDLTDRRLARIVRQSQDLPGYE
                     LFQYVDAEGARHGIDAGDVNDYLRHIAGEECTAKDFRTWAGTVLAAKALQECRAIGSA
                     AQAKRNIVAAIEVVAKRLGNTKTICRKCYVHPEIVQAYLEGSLARSLTTRIRRPFARM
                     AGCLTPDEAAVLACLERRLGRKASRGIGLRSSA"
BASE COUNT          834 a         1197 c         1141 g          908 t
ORIGIN      
        1 gagttcatgt gttattcctt cctgtacgac atgggcgtgg ctactcttag acggccgcgt
       61 ccttccgatc acggcaagct cgcaccaatt ccctggtgaa taccagtaat accgccacac
      121 gggtcctgcc agctctcgac agctaactct ttgaattcga acgacgcccc agtgaaggcg
      181 tcatcctcaa tactctggga gagttactag gctactagtg aaaatgtaag cggctggccg
      241 tcacgaaacc gtgacaggaa tattcctagt ttgctggcgg cgatttgcac caggaccgcg
      301 tacgctcacc gcacagcgag ttccagcaat gcacctgggt ggagccgtcc cttgcatact
      361 ccatgtgacc tgagtctctt gatcgacggc ctgtcgtcga cggtcacgcg ggccgacatc
      421 gtcgcgttgt tttcgtcttg tggcaaggtc acggcagcgg agatcgtcat tgatgcggac
      481 ggcatgcctc tgggagttgc gcgcgtgacc atgtcgacga aagaggaagc acggttcgca
      541 atccgcacgt ttcaccgttg ccgattcaga ggtcacacgc tcctggtgtt cgaagatacc
      601 acgaagaacc agcgggagga tctatggaac agtcgcatgt tcaaacggcc ctgaccgaga
      661 aggagactgc ccctgcgacg caatgtccgc gctgtgcgtc acgggtgttg ttctggtgga
      721 ccaatcccaa agggcacacg gtcacctggt tctgccctga atgccgggcc gtcaagacgc
      781 gcccgttacc cgtatcgaac cgggaaattt ctcacgcgaa ggcgcgatga gtactcgttt
      841 gacgaatgag ctctaggtgc gtgactgtgg gggatctctg cggtcttgtc aaataagcgc
      901 atgttccagc gcataacgcg tcagctcggc gttggtcttc agccggagct tctggagaat
      961 acgggtccgg taggtactga cggtattggc gctgacacag agtgtgagtg caatctgctt
     1021 gagcgattca ccctgcgcga ccatcctgag aatttgaaat tcccgattgg acagggcatc
     1081 gtgaagttga ccggaatcct tggtgcgcat gcgctcaatc aaacagcgcg ccaccgccgg
     1141 attcacgaat tcctgccctg cgaggattga ccgcatagcc gccacgagtt catcgagtcc
     1201 cgtcgatttg ttcaggtacc cgctggcccc gcttcggatc atccgcacgg cataactttc
     1261 ttcagagtgc atgctgtaca caagaacagg cgtcgaggga tgcaagctgc ggatgtgctc
     1321 caacagatcg gtcccggact tcccgggcat cgtgacgtcc atgatgaacg catcccattt
     1381 cccttgggac gccagcgtca ccatctccca gcccgacttg gcctcatgca cctcgagcgg
     1441 cgcaaagtgc tcttgcagga catctttggt cccccggcga aagacggcat gatcgtcccc
     1501 gatcaatatt ctcaacatag gcgcaaccct gtgttgtgag tcatgttcac gatggctgca
     1561 cgtcagtggc atatgttctg tgattacttt accggaaagg agtcacccaa aacgaggggc
     1621 tatttcttaa gtgatctcca gctagaaaaa catcggcgtg aagaccgtag gatgagccga
     1681 acgggcgtac tcgtaggcca atgctgacgc ctggacgcgc gatccgctga cgggcgcgaa
     1741 aggcgtgtag aaatgttgca taagggctca cgccgggata gcacaacaga taccagcgta
     1801 ctaattgggc tgacgtacga gggatacgcc tagtggcttc tcgagcacgt caatgcatag
     1861 ataacaacgg ccccaacggc gatctatttt tcttcccacg ttgttgcgtt tgcgtgatcc
     1921 gaaatgatgg aaaccgtcca tatcgtaccg aacgaacagc cgacgtacga ggaatcactg
     1981 cggctgggct gggccctgct gtggcgcggc gtgggtagct tcgttctcct catgttcgct
     2041 ggaaacatcg gcttgctctt tctcattcct gaattgacgc ggacagttcc gacactgtgg
     2101 attgccctgt tgcccctggc ctttgccacc ttgatcagtg cctttctcat catgccgttt
     2161 gttgtccggc gattggtgaa ggcgccattc aaggggttcc acctgagctt cgtacgagat
     2221 cgacttctcg gtcagtaact cgagaggatg gattggtctt cctcgctgtt cccaacaagc
     2281 ggcggcacaa cgaactcaag gccgcttaac atctaggaag gagtccagcg gctggcagtg
     2341 cgtcgataca gccagacgtc gatctacttc cagcagcgcg acgggcttga accggcaacc
     2401 cagagtgatt tcaacgccat agatgaaaat ggcccgacac cggcgacatg caatccccaa
     2461 tgtggaggtc acccatgctc tggctcttca ccactcacgg ctctgtggga atgttcatcg
     2521 tgcgtgtgac cctggcgatt gtgatctttg cccatggggc acagaaactc ctgggctggt
     2581 ttgggggaca gggctttacg ggcacgattg gattttttac cacttccatg ggccttccct
     2641 ggctcatagc ctttctcgtc atcatcggcg agtcgatcgg cagcctcagt ctgctggccg
     2701 ggttcctcac tcgctttgtg gctgccagct acctgctcat catgctggga gctgttgcca
     2761 tggtgcactg gccaaacggc ttcttcatga attggtttgg gcaacagcag ggcgagggat
     2821 ttgaatatca cctgttgatc atcgggatga gtgtggcact tcttctggaa ggtggcgggg
     2881 cctggtctgt cgaccacctg atcgccaggt ggatggccca acatggatgg gccgggactc
     2941 agccattaca tgcctttgcc cagagcaaac aataaccagc cccacggctg aacaggcgaa
     3001 agagacccgt gagcaggtgt cggaggtatg ccgccccgat cagagacgtc ctgctcgccc
     3061 caccctggaa tgtctgcgag cgatccgcag gtaagacagc ggagcccctc gcaggaccag
     3121 gctaatgacg agcccatgcg cgcatgagca ctgtggcaga tctggaatct cccacatcgt
     3181 atacaagtca ccgccatata ccctgacatt tgatttatcc aatgcgccta cgccttctct
     3241 gacgaatgcg gctccgttgc aaagatcttg gtacacatca atggggccgg atgcacggcc
     3301 gggacacgca tcctcctctc ccttatgccg atgagcgcaa tcctatgccc cgactggcct
     3361 tgcgcccgag gcggcgctca agacacgcta gtaccgccgc ctcatctggc gtgagacatc
     3421 ccgccatgcg tgcgaaaggt cgccggattc tcgtcgtcaa ggatcgagcc aacgatcctt
     3481 ccaagtaggc ctgcacgatt tccggatgca cataacattt ccggcagatg gtcttggtat
     3541 tgcccagccg cttcgcgact acctcgatgg cagccacgat attgcgtttc gcctgggctg
     3601 ccgatccgat ggcgcggcat tcttgcaacg ccttcgcggc cagcacggtc ccggcccagg
     3661 tgcgaaagtc cttcgccgta cactcctccc cggctatgtg ccggagataa tcattgacgt
     3721 ctcccgcatc tatgccatgt cgggcgccct ccgcatcgac gtattgaaag agttcgtatc
     3781 ccggcagatc ctggctttgt cgcacgatgc gggccaaccg gcggtccgtc agatccacca
     3841 gatgttgcac accgctcttg cctcgaaact cgaatcggac tcttgcccct tcgacgcggg
     3901 cgtggcgatc acgcatcgtg gtcagtccga acgaattatt ggttttggca tattcctcat
     3961 tgccgactct gatcagcgtg gtttcgagca ggcgtacgac tgtggctaaa atcttttctc
     4021 gtggcaaacc cggcaaggca aggtcccgct cgatgcgttg ccggatgcgc ggcaacagcc
//
LOCUS       DAOQEW010000290         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9861_length_4030_cov_7.407547, whole genome shotgun sequence.
ACCESSION   DAOQEW010000290 DAOQEW010000000
VERSION     DAOQEW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9861_length_4030_cov_7.407547"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1196)
                     /locus_tag="PLS35_18735"
     CDS             complement(42..1196)
                     /locus_tag="PLS35_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNI20764.1"
                     /translation="MKKSDRTARLAQSDIRAMTLACAKVNGINMSQGVCDTPVPPVVV
                     QGAQQAMSQGHNIYARFDGIAELRQAIADKVASFNRITADPETNVTVSAGATGSFQAT
                     CMALLNPGDEVILFEPFYAYHVQAILAVEGVPRYVTMRPPDWTMDVKSLEQAVTSKTK
                     AIVVNTPGNPSGKVFTRRELEQIAEIACRRDLSVITDEIYEYFVFDGREHVSMASLPG
                     MADRTITIGGYSKTFSITGWRIGYSVAEASWAKAIGAMSDVLYVCAPTPLQYGVAAGI
                     RALGPSYYKELALDHQRKRDRFCGALAKAGLPPAVPQGAYYVLADVSRLPGNTSRERA
                     MYLLDKTGVAGVPGEAFFEGTEGSRFMRFCMAKTDADLERASQAIERLTS"
     gene            complement(1357..1938)
                     /locus_tag="PLS35_18740"
     CDS             complement(1357..1938)
                     /locus_tag="PLS35_18740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNI20765.1"
                     /translation="MARALFPLAVAGCLMALMPPASRLAFDPIMKEVAGRPTAEPIAS
                     LIATYAEQYALDPALLQAVIKVESNFNSDAVSSKGAIGLMQLMPLTAAAFHVLDPFDP
                     KDNIRAGAALLRGLLDRFGGDLSLALAAYHLGEARVRQAIGVPALPATQLYVNRVLGH
                     YDRFRAGTSRLPTRVTTRREERRTLWSLQQAPE"
     gene            complement(2006..2392)
                     /locus_tag="PLS35_18745"
     CDS             complement(2006..2392)
                     /locus_tag="PLS35_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20766.1"
                     /translation="MMRLLADCRSLMLVLPLMVTATGCAESALQGHSPQEDLGMGIVS
                     GTLGAQSRSAVSSGEQSTASVVGQVQAVEGGAYLIRDVRGQEHRIPHDENTRIDRPAH
                     VGDRIQSWFDRHGRAVLIRSLDEEGR"
     assembly_gap    2510..2609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2610..2737)
                     /locus_tag="PLS35_18750"
     CDS             complement(<2610..2737)
                     /locus_tag="PLS35_18750"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNI20767.1"
                     /translation="MVGLSSNPARPSYRVASYMQQQGKVVIPVNPRETDVLGERAYP"
     gene            complement(2800..3420)
                     /gene="msrA"
                     /locus_tag="PLS35_18755"
     CDS             complement(2800..3420)
                     /gene="msrA"
                     /locus_tag="PLS35_18755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNI20768.1"
                     /translation="MRSIIGRFGALGVLPMLLAGLLVSQSQGADPSGAAKAYFAGGCF
                     WCMEEVFEKVPGVTAVVSGYMGGRVEHPSYEQVSAGGTGHAESVEVVYDPTKASYTTL
                     LDAFWHNVDPVTPNAQFCDHGSQYRSVIFYQGEEQKRLAEESKRAIEQSQRLTQPIVT
                     ELTKASQFYPAEEYHQDFYKKNPIRYKFYKFNCGRAQRLEEVWGAP"
     gene            3559..>4030
                     /locus_tag="PLS35_18760"
     CDS             3559..>4030
                     /locus_tag="PLS35_18760"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNI20769.1"
                     /translation="MTYLRGVAGLLTALMVSGCAFWMMKGEPPEVLVTNVTPLEASAF
                     EQRLQVDLRIRNPNDFDLAVTGIDFKLDLNGNRLARGLGNKELVVPRLSDAVTSVETS
                     TSTLQVVRQLLSFSGNQPLTYHLTGVVHTKAGRLPFDNSGVLLDTGAVSSSPAAQ"
BASE COUNT          811 a         1209 c         1136 g          774 t
ORIGIN      
        1 tcctggcagg cgcggtaagg atgctggtgc tgtcactgag tttatgaggt caatcgttcg
       61 atcgcctggc tggcccgctc gaggtcggcg tcggtcttcg ccatacaaaa ccgcataaag
      121 cgactgccct ccgtcccttc aaaaaatgct tcgcccggca cgcccgctac accggtcttg
      181 tccaacaaat acatcgcccg ctcccgactg gtgtttcccg ggaggcggga tacgtcggcc
      241 agcacgtagt aggctccttg cggcacggct ggaggcaagc ctgctttcgc taaagccccg
      301 cagaaccgat cgcgcttccg ttggtggtcc agggccagtt ccttgtaata cgaaggaccg
      361 agtgcgcgga tgccggcggc cacaccgtac tgtaagggcg tgggagcgca gacatacaac
      421 acatcactca tcgcgccgat ggccttcgcc caggaagcct ccgccacgct gtagccgatg
      481 cgccatccgg tgatgctgaa ggttttggaa tagccgccga tggtaatggt ccgatcggcc
      541 atgccgggca atgaggccat gctcacgtgt tcccgcccgt cgaagacaaa atactcatag
      601 atttcgtccg tgatcactga caggtcacgg cggcaggcga tctcagcgat ctgctccaat
      661 tcacgccgcg tgaagacctt gccggatgga ttccccggcg tattgaccac gatggctttg
      721 gtcttggacg tcacggcctg ttccaggctc ttcacgtcca tcgtccaatc cggcggccgc
      781 atcgtcacat accggggcac gccctccacg gcgagaatcg cctggacgtg gtaagcataa
      841 aacggttcaa acagaatcac ttcatcgcca gggttgagga gcgccatgca ggtggcttgg
      901 aacgatccgg tcgcgccggc actcaccgtc acattggtct ccggatcggc ggtgattcga
      961 ttgaacgacg ccaccttgtc tgcgattgcc tgccgcagtt ccgcaatgcc atcaaaccgg
     1021 gcataaatat tgtgtccctg cgacatcgcc tgctgcgcgc cttgcacgac caccggaggg
     1081 acaggagtgt cgcacacccc ttgagacata ttgatgccgt tgacctttgc acaggctagg
     1141 gtcatcgctc tgatatcgga ttgagccaat cgggctgtac gatcactctt cttcatcaca
     1201 ccctcaatgt ccaggtcatg tcttgattca tgaagtttgc ggatcctgcc tcgcaaggca
     1261 gatgtcccta cgccgttccc acaaaaaatg caaccggcaa acagccatcg tgggttctgg
     1321 aaagagccaa ccgccttgcg aatcagcgcg aggccgttat tcaggagcct gctgcagaga
     1381 ccaaagcgta cgacgttctt cccggcgcgt cgtcacacgc gtgggaagcc ggctggttcc
     1441 ggcacgaaaa cggtcatagt ggccgagtac ccgattgaca tagagctgcg ttgcaggcag
     1501 cgccggtaca ccgatggcct gcctgacgcg cgcctcgccg agatgatagg ctgccaacgc
     1561 caacgacagg tctccaccga accggtccag caatccgcga agcagcgcag cacccgcacg
     1621 gatgttgtct ttcggatcga atggatccaa cacgtggaac gctgccgccg tcaacggcat
     1681 cagctgcatg agcccaatcg cccctttcga ggacacggcg tccgaattaa aattcgactc
     1741 gaccttgatg accgcctgca ggagcgcagg gtcgagggca tattgctcgg cataggtcgc
     1801 aatgagcgag gcaatgggtt cggcggtagg ccggcccgcc acctccttca tgatcgggtc
     1861 gaaggccagc cggctggccg gaggcatcag ggccatcagg catcccgcca ccgctaaagg
     1921 aaagagtgca cgggccactc ggctccagcg ggtattccat tcaggctgtt gcatcgttgg
     1981 gagaagcggt ctcgaccgga cagccctagc ggccttcctc atcaaggctc cgaatgagga
     2041 cggcacggcc atgccgatcg aaccaggatt gaatgcggtc gcctacgtgg gccgggcgat
     2101 cgattctggt gttttcgtca tggggaatgc gatgttcctg tccgcggaca tcccgaatca
     2161 ggtaggcgcc gccctcgacg gcctgcactt ggccgacgac tgaggcggtg gattgctcgc
     2221 ccgaggaaac ggcagaccgg ctttgcgcgc ccaaggttcc ggatacgatc cccatcccga
     2281 ggtcttcctg aggggaatgt ccctggagcg cagactcggc gcagccggtc gcagtaacca
     2341 tcaagggcag gacaagcatc agagagcgac aatccgcgag caaacgcatc atggccggaa
     2401 cctccatcga tgaatgacgt ggtgaacggt gcaggacggg tgtgagcgca ccgtcacgtc
     2461 actattccat ggagcggcgg aatgctcaag gaaatgaggg aaactgacgn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gataggcccg ttcacctaat acatcagtct
     2641 cgcgcggatt caccgggatc accaccttgc cctgctgctg catgtagctg gccacacgat
     2701 aggacgggcg ggccggattc gatgataatc cgaccacagc aatagtacgg caatctcgca
     2761 agagacgtgc gatctcctcc cgggaggaag cgtgatccgt cacggtgcac cccagacttc
     2821 ttcgagccgc tgcgcgcgac cgcaattaaa tttatagaac ttataccgga tcggattctt
     2881 cttataaaaa tcctggtggt actcttcggc cggataaaac tgcgaggcct tggtcagttc
     2941 ggtgacgatc ggctgcgtca gccgttggga ctgttcaatc gcgcgctttg actcctccgc
     3001 cagtcgcttc tgctcttccc cttggtagaa gatgacggac cggtactgac tgccatggtc
     3061 acaaaactgc gcgttcggcg tgaccgggtc cacattgtgc caaaaggcat cgagcaacgt
     3121 ggtgtaactc gccttggtcg ggtcatacac cacttcaacg gactcggcat gccctgttcc
     3181 ccctgcagac acctgctcgt aactgggatg ctcgactcgt ccgcccatat atccgctgac
     3241 cacagccgtg acgccgggaa ccttctcgaa cacttcctcc atgcaccaga aacagcctcc
     3301 ggcgaaatag gctttggcgg cgccagacgg atcggccccc tgactctgcg agaccagcaa
     3361 tcctgcgagc agcatcggca acaccccgag ggcaccgaac cgacctatga tggacctcat
     3421 tggacgcctc ctctcaaagc tgagcgactt tcgatcatca taccagagca tagggaccgc
     3481 cgcgatccgg acagcgtccc cttcttcaac tctttacgtg cgtcgcaatc gattggatgg
     3541 gtgtgtatga taggcagcat gacctacctg cgtggagtgg cgggactact aacggcgctg
     3601 atggtttccg gttgcgcctt ctggatgatg aaaggcgagc caccggaagt gctggtcacg
     3661 aatgtgacgc cgctggaggc gagtgccttt gagcagcgac ttcaggttga tcttcgtatc
     3721 cggaatccca acgactttga cctcgccgtc acagggatcg atttcaagct ggatctgaat
     3781 ggaaaccggc tcgcgcgtgg gttggggaac aaagaattgg tcgtgccgcg cttgagcgac
     3841 gcggtgacgt ccgtcgaaac cagcacgtcc accctccagg tcgtcaggca gctgttgtcg
     3901 ttctccggca atcagccgct gacttatcac ctgacaggcg tggtccacac caaggcaggc
     3961 cgcttgcctt tcgataactc cggcgtgctc ctggacacag gagccgtgtc cagctctccc
     4021 gctgcccagt
//
LOCUS       DAOQEW010000291         4008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_9969_length_4008_cov_6.329623, whole genome shotgun sequence.
ACCESSION   DAOQEW010000291 DAOQEW010000000
VERSION     DAOQEW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9969_length_4008_cov_6.329623"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLS35_18765"
     CDS             complement(<1..397)
                     /locus_tag="PLS35_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20770.1"
                     /translation="MPAPGDLIHQQSTSTGTGNITLSSVNGKRTFNDEFGTGGTDKFD
                     YFISNQAAAEWERGTGHLSASTTLVRDTVLASSNANAAVNFSAGTKDIANDVPAALQV
                     RGPGSATVGNIALWDSSAGNLLKDAGYAPR"
     gene            complement(397..804)
                     /locus_tag="PLS35_18770"
     CDS             complement(397..804)
                     /locus_tag="PLS35_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20771.1"
                     /translation="MAVKFSVAVRNARLDALETAIGTSAVLKIRSGSAPTNITDADSG
                     TVLATLSLPSDWMANAASGSKSKSGTWQDTSADNTGTAAHFRLYASDGTTQHMQGSIT
                     ATGGGGDMTVDNTSFVAGQSFTVTAFTLTDGNG"
     gene            complement(804..971)
                     /locus_tag="PLS35_18775"
     CDS             complement(804..971)
                     /locus_tag="PLS35_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20772.1"
                     /translation="MNIQNMDFMQIVHLAGRVVCALGLLMFFFNVNLGVQMLAHPGIE
                     VAAAGWLMKAL"
     gene            complement(968..2128)
                     /locus_tag="PLS35_18780"
     CDS             complement(968..2128)
                     /locus_tag="PLS35_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20773.1"
                     /translation="MSTIPAPARRGLIPAVSGLFSAVSRPASRINLDRIAIGLVFFAG
                     VVTILKKNGVDVPSFMVLFGCSLGIAGLLYEMSASRSMMRAFWEAKPFSMFCNLTIWV
                     AAFSFSIFNWIGAAAEGQAEKTNIQKAAFVHSADARTRLDMAQDALKKARNAAQEKHN
                     AAWETIPVVDGQKITDRGQAEALIAKIKSNTRYWNLTNECQTTKGPQTRKYCADYNDA
                     KAALSWADTHPALIEAANAADQDVKRLEQEVASARSEASSTKVVTSEDRADLFMLTDW
                     GGMSESRAQQLTGLISVLVISVFISFGSMRDEAARLTAEGPRRKWSFLSRTYRWAYRK
                     VWGQEPDNTTYVTNYLDPRGAEALAAVERDRERITHAARAAQARLNPLGAFA"
     gene            complement(2628..2822)
                     /locus_tag="PLS35_18785"
     CDS             complement(2628..2822)
                     /locus_tag="PLS35_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20774.1"
                     /translation="MAGSIRDRVRRAAGSQKTWAEATGQNVEHVSRIINGKYPVPEWW
                     EAMLELLEALPPKDRPNRWG"
     gene            3107..>4008
                     /locus_tag="PLS35_18790"
     CDS             3107..>4008
                     /locus_tag="PLS35_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20775.1"
                     /translation="MRTSIKGRALAIIAGLAFTAGALTILLGPALLTPLEWSSYHVLT
                     IISVFGTTAAGHLLADAGFKHPIAGLGFLVLFLGGTALVVANSVGRQAATFEESTLSA
                     EATNAAIADKSEDLKQARARKVYADAQVQRETSRGGCGRSCKDWKTNASDVAIVIGQL
                     ERDIAALGPKKPVNAKAERMAEMAALFGVSKAKAQAMFVLLEPLLNCLFFELGSIVSL
                     GFAFRQGKRPVLVAANDQLPSQIKSFASVADNRQTSFAYELPDPEPPKGKKPRELPKG
                     VIEFSNHPVIRALTDAGGSVRSHR"
BASE COUNT          807 a         1177 c         1192 g          832 t
ORIGIN      
        1 cgcgcggagc atagcccgca tccttcaaca gattccctgc cgaagaatcc caaagagcta
       61 tgttcccgac tgtggcgctc ccgggtcctc gaacctgaag cgcagcaggg acatcgttcg
      121 cgatgtcctt cgtgccggca gagaagttga cggccgcgtt cgcgtttgaa ctggcaagga
      181 ccgtgtccct gacaagcgtc gtcgacgccg agaggtggcc ggttccccgc tcccattccg
      241 ctgcggcttg gttcgatatg aaatagtcga acttgtccgt gccgccggtg ccgaactcat
      301 cattgaacgt gcgcttaccg ttgacagacg acaacgtgat gttgcccgtt ccggtggacg
      361 tcgactgttg gtggatgaga tcgccggggg cgggcatcag ccgttgccat ccgtcaacgt
      421 gaatgccgtg actgtgaagc tctgtcccgc tacaaaggac gtgttatcga cggtcatgtc
      481 gccgccaccg cctgtagcgg tgattgaccc ctgcatatgc tgggtcgttc cgtcagaagc
      541 atagagccgg aaatgtgccg ccgtaccggt gttgtcagcg gatgtgtcct gccaagtgcc
      601 ggacttcgat ttcgagccag acgccgcgtt ggccatccaa tcagatggca gcgagagggt
      661 cgcgagaacg gttccggaat cggcatcggt aatgttggtc ggagcagaac cggagcggat
      721 cttcagaaca gccgatgttc caatcgccgt ttccagagca tcgagccggg cattgcggac
      781 cgccacggag aatttaactg ccatcagagg gccttcatca accagccggc ggccgcaacc
      841 tcaatcccgg gatgcgcgag catctgcaca ccgaggttga cgttgaagaa aaacatcaaa
      901 agaccgagcg cgcacacgac gcgcccggcc aagtggacga tctgcatgaa gtccatgttc
      961 tgaatgttca tgcaaacgct cccagcggat tgagacgggc ttgagccgcg cgcgccgcgt
     1021 gggtaatacg ctcccgatct cgttctactg ccgccagtgc ttccgctccg cgcggatcaa
     1081 ggtagttcgt cacgtaagtc gtgttgtcgg gctcctggcc ccagaccttg cgataagccc
     1141 agcgataggt gcgcgacagg aaagaccatt tccggcgcgg accttccgcc gtcaggcgtg
     1201 ccgcttcatc gcgcatagat ccgaacgaga tgaacaccga aatgacgaga accgagatca
     1261 ggccagtcaa ctgctgcgcg cggctttcgc tcatgccgcc ccagtccgtc agcatgaaca
     1321 ggtcggcgcg atcttccgag gtcacaacct tcgtgctcga tgcttcgctg cgggcgcttg
     1381 ccacttcttg ctcgagacgc ttcacgtcct ggtctgctgc gtttgccgcc tcgatcagag
     1441 ccgggtgcgt atcagcccac gaaagagcgg ccttcgcatc gttgtagtcg gcgcagtact
     1501 tccgggtctg cggacctttg gtcgtctggc actcgttcgt cagattccag taccgagtgt
     1561 tcgatttgat cttcgctatc aaggcttcgg cctgaccacg gtcggtgatc ttctggccat
     1621 cgacgacagg gatcgtctcc caagcggcat tgtgcttttc ctgggcggcg ttgcgggctt
     1681 tcttcaaggc gtcctgagcc atgtcgagac gcgtgcgggc atccgccgag tgcacgaagg
     1741 ctgccttctg aatgttcgtc ttctcagcct gcccctcagc cgcggcgccg atccagttga
     1801 agatcgagaa cgagaaagcc gcaacccaga tcgtcaggtt gcagaacatc gagaagggct
     1861 tggcttccca gaaggcccgc atcatcgatc gggatgcgct catctcgtag agcagacctg
     1921 cgatgccgag tgagcagccg aacagcacca tgaagctcgg gacgtcgacg ccgttcttct
     1981 tcaggatcgt gacgacgcca gcgaagaaga cgagaccaat ggcgatgcga tcaagattga
     2041 tccgggaggc cgggcgcgag actgcggaaa agaggccgga gacagccggg atcaggcccc
     2101 ggcgggccgg cgcaggaatg gtgctcatgt cgggggctcc tgagatagga ggtgaatttg
     2161 caaaaacaga acccccgccg ccgcgcatcc atggcgttgg atcgggtgcc agagcatgtg
     2221 tcgtgacaat cccgggaagg agacagtcac ggcgacgggg atttcaggat tgtttggggg
     2281 tcgttttgct cgcatttccg gattcggcgg cccggattgc gggtaccacc gttggaaatg
     2341 cgagcctggc cttgcgatac tgggctttgt taagctcgcg gtcggccgac aagaaacaga
     2401 aaaagccgat acgccacgat agaatctagc acgagcgatg gagtgatttg ccttatgcgt
     2461 caaggcctat cccagaggac cgcggcgcac gccgcacatg cgtgacatgg catccacagt
     2521 gcgctttggt agctcagccg atatcacttt cctcttaatc gaaggcggct tgtattcgcc
     2581 aacttccggg cgagcgaatt cgccgaaata ttgcgcggcc tttcatgtta gccccatcga
     2641 tttgggcggt cttttggagg cagcgcctca agcaattcca gcattgcttc ccaccattcc
     2701 ggcacggggt atttgccgtt gatgatgcgg gagacgtgct cgacgttctg cccggtcgct
     2761 tccgcccagg ttttttgtga acccgccgcg cgccgcactc gatctctgat acttcctgcc
     2821 atttcccatt ggtatggcaa aaatgtcgtg gcgtcaagta aatcgtatgc tagatgggcg
     2881 ggaacttgac gtagcgtcaa ttaaatgcga ttataacaat cgagccccgc aaccgtgcaa
     2941 ccggcggcag ggctcttgat ctcaagcctt acacagaagg attgaaaccc atgtccgcac
     3001 tagcattcat cttagtcttc gtcacggcat caccagttct tattttaggc gccgttactg
     3061 ttctcagcct cctcgtagaa gcgatgatcg aggggagcat cggcaaatga gaacctccat
     3121 caaagggcga gcactcgcca tcatcgctgg tctggcgttc actgccggcg ctttgaccat
     3181 tcttcttggg ccagccttac tgacgccgtt ggaatggtcc tcgtaccatg tcctgaccat
     3241 catcagcgtc tttggaacga cagcggccgg acatctcctt gccgatgccg gattcaagca
     3301 cccgatcgca ggactgggct tcctggttct gttcctaggc ggcaccgcgc tcgtggttgc
     3361 caacagtgtt ggccggcagg ctgcaacatt cgaagaaagc accctgtctg cggaagcgac
     3421 caacgccgcg attgccgaca agagtgaaga cctgaagcag gcccgggccc gcaaggtcta
     3481 tgcggatgct caggttcagc gtgagaccag ccggggcggc tgcggccgct cctgcaaaga
     3541 ctggaaaacc aacgcgtccg acgtggcgat cgtcatcggt caacttgaac gcgacatcgc
     3601 agcgctcggc ccgaagaagc cggtcaatgc gaaagccgag cggatggccg agatggcagc
     3661 cctgttcggc gtgagcaaag ccaaggcgca ggcaatgttc gttctgctgg agcctctgct
     3721 caattgcctg ttctttgaat tgggctccat cgtctcgctc ggcttcgcgt tccgccaggg
     3781 caagcgtccg gttttagttg ccgcaaacga tcaattgccg agtcagatca agtcgtttgc
     3841 ctcagttgcc gacaaccggc aaacgtcatt tgcctatgaa ttgccggacc ctgagccacc
     3901 gaaaggcaag aagccgcgag agttgccgaa gggtgttatc gagttcagca accatccggt
     3961 catcagggct cttacggacg cgggcgggtc agtacggtct catcgtga
//
LOCUS       DAOQEW010000292         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10094_length_3978_cov_8.774662, whole genome shotgun sequence.
ACCESSION   DAOQEW010000292 DAOQEW010000000
VERSION     DAOQEW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10094_length_3978_cov_8.774662"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1722
                     /gene="ybgF"
                     /locus_tag="PLS35_18795"
     CDS             46..1722
                     /gene="ybgF"
                     /locus_tag="PLS35_18795"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNI20776.1"
                     /translation="MDSEWPYRKTMTDQPSNAKRVTPGTYAQAARFGACVVVVTGCVL
                     LSGCVAQQADLKQTERELQRRIKQQTEEQAQNRARQNQEIVSLREQDIPSLRGDVDKA
                     LHRAQSLDARQDDLLAKVASQESKFERRIGEAEKRSAEEGKRLGWVEKQLVDQDAMLK
                     GERDRNRAELAAVTARLDQINSHIEAIQKNVLDAMQKTTTGLAQKVDSRLDEQQKLLH
                     GLETRSQNVAQLDAQNKVLGDQVGKFNQALVDFKQALGSLGERVVQQDQTVKHLAATL
                     EQDTVALSKRTDALAGKMDADNKVTADHFNEVNRSVASVAKALENAGGKFVSREDDHE
                     RRLDDTTRELTHVQAQIQTLDKNLENQHAFLKQVEQHLVALRANATQRSEPAPAVAEA
                     APLPQAIPAPVPPPAPAQTQDFASSSAITPRAENRSAALVADRESYERTLTRFKDGDL
                     DGARQGFAEFLVQHPHSDLAPNARFWLGESYYGKKDYSRAIDAYDQVQLNHPASEKVP
                     AALLKKGYAYLALKDRKKAASALKQVIDLYPRSPEANKAMDKLNQLKESH"
     gene            1725..2504
                     /gene="ybgF"
                     /locus_tag="PLS35_18800"
     CDS             1725..2504
                     /gene="ybgF"
                     /locus_tag="PLS35_18800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNI20777.1"
                     /translation="MKLRFAGALRVSAMLACGLVVAGCAKHADFLEIRDQVSIMARTQ
                     DQEQKRFEAMQRRLESLERVREPEGGKLRLDDAMTRLQKLEGRLAKVEEAQLAQAAAI
                     RSDAALAEASRQARISKPSGPIEPQAIVPGVPSITPTSAFNLAYNDYLNGKFDLAVNG
                     FQHFIKDFPSTSLTPNAHYWLGESYYGQKDYIRAIQSFEHVVNEYAGNEKVPAALFKL
                     GLSAAETGDTAKSRKYLKRVIEEYSTSDEAKLAKTKMAEIR"
     gene            2577..>3978
                     /gene="mutL"
                     /locus_tag="PLS35_18805"
     CDS             2577..>3978
                     /gene="mutL"
                     /locus_tag="PLS35_18805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNI20778.1"
                     /translation="MDIASSVGKIQVLPSDVIGRIAAGEVVERPAAVVKELLENSLDA
                     GSSAITVEIKDGGLGLIRVSDDGEGMSRRDASLAFERHATSKLQSDDQLGAVRTMGFR
                     GEALPSIAAVSKVRLTTVARHDQVGTQLWLTGGTLTRVEDAAAIPGTTIEVSELFFNT
                     PARRKFLKSTTTEFSHISHVVQQAALAWPQVYMRLVHNGYEVFALPAVSSPRDRVLQV
                     YRAAFGERALAVDVERDGLLLKGFIIDPVRARAGRTPQELFVNRRPIKNSTVQHAVID
                     GYSSFLAKGHAPLFVLFLDVEPHRVDVNVHPTKREVRFADTEQIHQLVRSAVRQTLGR
                     AQVEASMAGAAHAEMTGVIPPTLSFPERERMSFGEAPPEPMARSKASVTPPDSDESQT
                     SFVGEGATAYASPASTDIVALGQMNRTYLIAQVGNELQVVDQHTAHERVLFERLWRAW
                     QGRAVTSQPLLLPEPLE"
BASE COUNT          858 a         1130 c         1232 g          758 t
ORIGIN      
        1 gtgacatgat tctccggaac agacgccggt caagggagac ctgtcatgga cagtgaatgg
       61 ccttatcgaa agacgatgac tgatcaaccg tcgaacgcga agcgtgtgac gccgggcacc
      121 tatgcgcagg cagctcgatt cggtgcctgt gtcgtggtcg tgacgggctg tgtcttgttg
      181 tcgggctgtg tggcgcagca ggcggatctc aagcagaccg aacgagagct ccagcgtcgc
      241 atcaagcaac agacggaaga gcaggcccag aaccgggcac gccaaaatca ggaaatcgtg
      301 tcgttgcgcg agcaggacat tccatccctg cgcggcgatg tcgataaggc cctccaccgt
      361 gcacaaagtt tggatgcgcg gcaagacgac ctgctggcca aggtggccag ccaggaatcg
      421 aagttcgagc gtcgcatcgg cgaagccgag aagcggtcgg ctgaggaagg caagcgcttg
      481 ggctgggtcg aaaagcagct ggtcgatcaa gatgccatgc tcaaaggcga gcgagaccgg
      541 aaccgggctg agctggccgc ggtgaccgct cgtctggatc aaatcaacag ccatatcgag
      601 gccattcaga agaacgttct ggatgcgatg cagaaaacta ccaccgggct cgcgcaaaag
      661 gtcgattcgc gcctggatga acagcagaag ttactgcacg ggttggagac ccgttcccag
      721 aacgtcgcgc agctcgatgc ccagaataag gtcttgggcg atcaggtcgg gaaattcaat
      781 caggcactgg tcgacttcaa gcaggcgctg ggaagcttgg gcgaacgcgt ggtgcaacag
      841 gaccagaccg tcaagcattt agccgcgacc ctggagcagg ataccgtggc tttgagcaag
      901 cggaccgatg cgctggccgg aaaaatggat gccgataaca aagtcaccgc cgaccacttc
      961 aatgaggtca atcggagtgt cgcctcggtg gcaaaggcgc tggaaaatgc cggcggcaaa
     1021 tttgtgtccc gtgaggacga ccatgaacgg cgcctcgatg acacgactcg agaactgacg
     1081 catgtgcagg cacagatcca gacgctggac aagaatctcg aaaatcaaca tgcgttcctg
     1141 aagcaggtcg agcagcattt ggtcgccctg cgagcgaacg ccactcagcg cagcgaaccg
     1201 gctcccgccg tcgccgaagc ggcaccgctt ccccaggcga ttccggcccc ggttccaccc
     1261 ccggccccgg cgcagacaca ggacttcgca tcgtcatcgg ctatcacccc ccgtgcggaa
     1321 aatcgtagcg cggcgctggt ggcggatcgg gagtcctacg aacggacttt gacccgtttc
     1381 aaggatggcg atttggatgg cgcccgtcag gggtttgcgg agtttctcgt tcagcatccg
     1441 cattcggacc tggcccccaa cgcgcgcttt tggttgggcg agtcgtacta tggcaaaaag
     1501 gattattcgc gggccatcga tgcctatgat caagtgcagt tgaatcatcc tgccagcgaa
     1561 aaggtgccgg ccgctctctt gaaaaaaggg tatgcctatc tggcgctgaa agatcggaaa
     1621 aaggcggcgt ccgctctgaa acaagtgatt gacctgtatc cgaggtctcc agaggccaat
     1681 aaggccatgg ataaattgaa ccaactaaag gagtcgcact aacgatgaag ctccgattcg
     1741 cgggggcatt gcgggtgagt gccatgctgg cttgcgggct ggttgtggcg ggctgtgcaa
     1801 aacatgctga tttcctcgaa atccgggatc aggtctccat catggcccgg actcaggatc
     1861 aggagcagaa gcggttcgag gcgatgcagc gccggctgga atcgctcgag cgggtgcgtg
     1921 agccggaagg cgggaaactg cgtttagacg acgccatgac ccggcttcaa aagctggaag
     1981 ggcggttagc gaaggtagaa gaggcgcagc tcgctcaagc ggctgctatc cggtctgatg
     2041 ccgctctcgc agaagccagc cgccaggcgc gtatctcgaa accctcaggc ccgattgaac
     2101 ctcaagccat tgtgccggga gttccgtcga tcacgccgac ctcggcgttc aatctggcgt
     2161 acaacgacta cctcaacggg aagttcgatt tggccgtcaa cgggtttcag cacttcatca
     2221 aggattttcc ctctacctcg ctgacaccca atgcccatta ctggctcggg gagtcctact
     2281 acggtcagaa ggattatatc cgtgccatac agtcgtttga gcacgtggtc aatgagtacg
     2341 cgggaaatga gaaggtgccg gctgccctct ttaagctcgg gctctcggcg gctgaaaccg
     2401 gagataccgc aaaatctcgc aagtatctca aacgggtcat tgaggagtac tccacatcgg
     2461 acgaagccaa gcttgcgaag acgaagatgg ccgagattcg atgaccctga ggtgtttgtc
     2521 cggtctcctc tccgctgagt tatcctccca tcaactcgca gggggcgttt cttcccatgg
     2581 atatagccag tagcgtggga aagattcagg ttctccccag cgatgtcatc ggacgcatcg
     2641 cagccggcga agtggttgag cgcccggctg cggtagtgaa agagctcctc gaaaacagtc
     2701 tcgacgcggg cagtagcgcg atcaccgtcg aaatcaaaga cgggggcctc ggattgattc
     2761 gggtcagcga cgacggcgaa ggcatgtcgc gtcgcgacgc ctctctggcc ttcgagcgac
     2821 atgctaccag caagctccag tcggatgacc aactcggcgc ggttcgtacg atgggattcc
     2881 gcggagaagc cctgccgagc attgccgccg tctcgaaagt ccgtctgacg actgtggccc
     2941 gtcacgatca ggtcggcaca cagctgtggc ttacgggagg gaccctcact cgtgtagagg
     3001 atgcggccgc gatccccgga acgacgatcg aagtgtcgga gctctttttc aatacgccgg
     3061 cccgccgaaa atttctcaag tcgaccacca cggagttctc ccacatcagc catgtggttc
     3121 agcaggccgc gttagcctgg ccgcaggtgt acatgcggct cgtgcataac ggatacgaag
     3181 tgtttgcgct gcctgctgtg tcctcgccgc gcgatcgcgt gctccaggtg tatcgggcag
     3241 cgttcggtga gcgagccttg gctgtcgatg tcgagcgcga tggtctgttg ctgaaagggt
     3301 tcatcattga tcccgtccgg gcccgtgcgg gacggacgcc gcaggaatta tttgtcaatc
     3361 gtcgccccat taagaacagc acggtgcaac atgccgtcat cgacggctat agctcgttcc
     3421 tggccaaggg ccatgcaccg ctcttcgtgt tgtttctgga tgtcgagccg caccgcgtgg
     3481 atgtgaatgt gcatcccacg aaacgtgagg ttcggttcgc cgataccgag cagatccacc
     3541 agctggtccg ctctgccgta cgccagacgc tcggtcgggc acaggtggaa gcgtcgatgg
     3601 caggggcggc tcatgctgag atgaccggtg tgatcccgcc gactctctct ttccctgaaa
     3661 gggaacggat gtcctttggg gaagcgcccc ccgagccaat ggccagatcg aaggcttccg
     3721 tcacgccacc tgattccgat gagagccaaa cttctttcgt tggagaaggt gccacggcgt
     3781 acgcctctcc tgcatccacg gatatcgtcg cactcgggca gatgaaccgt acgtatttga
     3841 ttgcgcaagt cgggaacgaa ctccaggtcg tcgaccaaca tacggcgcat gagcgtgtgt
     3901 tgttcgaacg actctggcgg gcctggcagg ggcgcgcggt gacgtcccaa ccactgcttt
     3961 tgccggaacc gctcgagt
//
LOCUS       DAOQEW010000293         3957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10182_length_3957_cov_7.207842, whole genome shotgun sequence.
ACCESSION   DAOQEW010000293 DAOQEW010000000
VERSION     DAOQEW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_3957_cov_7.207842"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..559
                     /locus_tag="PLS35_18810"
     CDS             329..559
                     /locus_tag="PLS35_18810"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNI20779.1"
                     /translation="MSLCEPADVGELALIRSLLDGNGIPYAIQHEHIGALYPGVALLG
                     SRVMVSECDRQRAEILLSRLALQVREATSETD"
     gene            complement(605..1057)
                     /locus_tag="PLS35_18815"
     CDS             complement(605..1057)
                     /locus_tag="PLS35_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20780.1"
                     /translation="MTDQTTSADPVSSLPPTAEPTPFIRQRPVRIIINSFLALFVVAL
                     IAFRFVPGWLDPDFSKHIESHRVMVGMDRKQVLDSWGSPNTMNVTHTSDGLRREEWIF
                     EDWESPAVVKHRYLYFEEGKLIGGHFSGSDVRLPLPTTPEPVKPKGHP"
     gene            1226..1888
                     /locus_tag="PLS35_18820"
     CDS             1226..1888
                     /locus_tag="PLS35_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20781.1"
                     /translation="MGRGGWRRDRVWAWVTALGLCLLGYAGVDAEAGSLYVYTDAQGQ
                     AVLTDNLEQIPAAYRGRVRTMTGPDSSAPAPGPAPAIAGNKTSTSTSSGVIGDILSAV
                     AAKVGARTIKGLTAYQTAVVIVAGASSLILLLFIFLSANPAIRILCKVLLVVVGLSAL
                     YQVLAIDAPSLEALAGPPQQGAGQPVDNMLGRMRSQTEQSYRAQGERTASQLEPAEQP
                     AP"
     gene            complement(1897..2676)
                     /locus_tag="PLS35_18825"
     CDS             complement(1897..2676)
                     /locus_tag="PLS35_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20782.1"
                     /translation="MAHQTVTRLIICCLLFFSLLGPECPSLWAGPYFEDGYLGLTQTE
                     LHAKLGLPQAVRDRKSALRVFTYYPISDWEQYFKKVVSPENGEDVYTFKRDGIDVRYS
                     FSFTFDPNDHNEQRPLFVRLVDIEFSPPVALSRIPQLVPEFRPTEDASAPVFRSNIML
                     LFFSGSPSADARFIVREKGKDALDWSLAFQMFALQGLPDPLTSKALIDRLEISTQSLP
                     LVKLRQRNTHEPVLNPYSKAFSQQPPPAPAVKKIPVPTYAE"
     gene            2863..>3957
                     /locus_tag="PLS35_18830"
     CDS             2863..>3957
                     /locus_tag="PLS35_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNI20783.1"
                     /translation="MPVHDIRELLRERILILDGAMGTMIQRHKLDEAAFRGERFKDWK
                     KDLKGHNDLLNVTQPSVIEAIHRQYLEAGADIVETNTFNSQSVSLADYGMEDLGYELS
                     KAGAECAKRAVAQVVKAQPARRCFVAGAIGPTTKTSSVSTDSNDAAARGTTYPELVQA
                     YGEQVRGLLDGGVDLLLVETIFDTLNAKAAFFAIQQLFAEGARQVPIMASVTFIQAGS
                     NRGFSGQTVEGFWNSISHVPLLSVGMNCALGPKEMRPLIEELSQLAPIFVSSHPNAGL
                     PNPLLPTGFPETPESLAPQLREWAQNGWLNIVGGCCGTTPDHIHTIAEAVRGVSPRRP
                     AKVEPFLRLSGLEALTVRPESNFVNVGERTN"
BASE COUNT          803 a         1080 c         1261 g          813 t
ORIGIN      
        1 gtgttctgac cgtgtcgggc gggcccggga aggaacggcg cgttcagatt accgagaccc
       61 cgtaagttgt ccgttgtccc ccttcggcaa ggacatcaac gccgatctcc gcccagtcat
      121 tgctccatat ccaaggccag cttctgcttc cggacgcggt gtgcgcttcc gtgagtgtat
      181 tgaccatccg aaggaattgc aacgaatcac gcgctgttcg cagtccgggg cgcctggtcc
      241 tgtccattcc gtttggtaaa tgtgcaaggg tgtctggcga gaggccattg tgacattacg
      301 gcatcatcct aggaatcttc agcctctggt gagcctctgc gaaccggcag atgtgggtga
      361 attggccctg atccggagcc tgctcgacgg caatggcatt ccctacgcca ttcagcatga
      421 acacatcggc gcactctatc ccggggtggc gctcttgggc agccgcgtta tggtgtcgga
      481 atgtgaccgg caacgggcgg agattctgtt gagccgcctg gccttgcagg tccgtgaggc
      541 caccagcgag acggattgaa acgggatcag gtggtcctgg cccggagtgg tcgttaggtg
      601 aggcttacgg gtggcctttg ggtttgaccg gttctggagt ggtgggcagg ggcaggcgca
      661 cgtcggatcc tgaaaaatgg ccgccgatca acttgccctc ttcaaagtag agatagcggt
      721 gcttgacgac cgccgggctt tcccaatctt caaagatcca ttcttcgcgc cgtagcccat
      781 cgctggtatg ggtgacgttc atcgtgttcg gcgaccccca cgaatccagg acctgtttgc
      841 gatccatccc caccatcacg cgatggcttt cgatatgttt ggagaaatcc gggtccagcc
      901 acccgggcac gaagcgaaac gcgatcaagg cgaccacgaa cagcgccagg aacgaattaa
      961 tgatgatcct gaccggccgc tggcggataa acggtgttgg ctccgctgtc gggggtaggg
     1021 aggaaacggg gtctgccgaa gtcgtctgat cggtcacaaa tatccttcca tcaacgagtg
     1081 ggcgagtgcg aaatgcctgc tgcatgctaa acaataggtt ttcggtccgt caagggaggg
     1141 aatcaccctc aagaaagggc atttcgccgc ctgtaccccg gtttcgacct gctatgctta
     1201 acggaggacc gaaggagggc gaaaaatggg gcgtggcggc tggaggcggg accgggtgtg
     1261 ggcgtgggtg actgcactgg gcctttgtct cctgggatat gcaggggttg atgccgaggc
     1321 gggctctctc tacgtctata ctgatgccca gggccaagcc gtcttgaccg acaatctcga
     1381 gcagataccg gccgcctatc ggggccgggt gcgtacgatg actggtccgg actcctcggc
     1441 tcctgctcct ggacccgcac cagcaatcgc cgggaataag acatctacgt ctacgtcctc
     1501 cggcgtgatt ggggatatcc tcagtgccgt ggcggcaaaa gttggcgcgc gaacgatcaa
     1561 agggctgacg gcctatcaaa cggccgtcgt gattgtggca ggcgcttctt ccctgatcct
     1621 gctcttgttt atttttttga gtgccaatcc tgccatcagg attctttgca aggtgctgct
     1681 ggtagtggtg ggactgtccg ctctctacca ggtcctggcg atcgatgcgc cgtccctgga
     1741 ggcgcttgcg ggacctcctc aacagggggc aggtcagccg gtggacaata tgttgggacg
     1801 gatgcgaagc cagaccgagc agagttaccg cgcacagggt gaacgcacgg ccagccagtt
     1861 ggagccagca gagcaaccag ctccctgaca cgcgtgctac tcggcgtaag tgggtaccgg
     1921 aatctttttc acagcaggcg cgggcggcgg ctgctgtgag aaggccttgg agtagggatt
     1981 caggaccggt tcgtgggtgt tgcgctggcg cagtttgacc agaggcaggc tttgggtact
     2041 gatttccagg cggtcgatca gcgcttttga ggtcagcgga tccggcagac cttgtaacgc
     2101 gaacatctgg aaggcgagtg accagtcgag tgcgtccttt cctttctccc gcacaatgaa
     2161 ccgcgcatcg gccgacgggc tccccgaaaa aaacaacaac atgatgttgg agcggaacac
     2221 cggcgcggag gcgtcctccg ttggtctgaa ttcaggaacg agttgaggaa tcctgctcaa
     2281 ggccaccggc ggagagaatt cgatatcgac gaggcggaca aacaggggcc gctgttcgtt
     2341 atgatcgttg ggatcaaacg taaagctgaa ggaataccgg acatcgatgc cgtcccgctt
     2401 gaaggtgtag acatcttcgc cgttttcagg ggagacgact ttcttgaagt attgctccca
     2461 gtcgctgatg gggtaatagg tgaagacgcg caaggcggat ttgcggtccc gtacggcctg
     2521 gggcagcccg agtttggcgt gtaattcggt ttgagtcagc ccgagatagc catcctcaaa
     2581 atacgggccg gcccacaggg aagggcattc cggtccgagg aggctgaaga aaagaaggca
     2641 acaaataatg aggcgggtga ctgtctgatg cgccatacaa tcgactcagg ctcggcccag
     2701 acactgtgtg gcatcgtatc ggccggggta gtcattgtca aggcggagag cccgtcatgg
     2761 cttgctgaaa aaagtccgtt cccgatatga tgcccgctca atggcagtca tcgcccgagt
     2821 cgccgcacgc ctgaagtcgg cgacagaaag acataggtag tcatgccggt acacgatatt
     2881 cgagagctgc tccgtgaacg tatcctgatc ctcgacgggg ccatggggac catgatccag
     2941 cgtcataaac tggacgaagc cgcgtttcgc ggggagcggt tcaaggattg gaaaaaagac
     3001 ctcaagggac acaacgacct gttgaacgtc actcaaccga gtgtgattga ggcaatccat
     3061 cggcagtatc tcgaagccgg ggcggacatt gtcgaaacca ataccttcaa ttctcaatcc
     3121 gtgtcactgg ccgactacgg aatggaagat cttggctatg agttgtccaa agccggagcg
     3181 gaatgtgcca agcgggcggt cgctcaggtc gtgaaggcgc agcctgcgcg gcgctgtttc
     3241 gtggcgggcg cgatcgggcc gaccacaaag acctcgtcag tgtcgacaga ctccaatgac
     3301 gcggcagcac gcgggaccac gtatccggaa ttggtgcagg cctatggcga gcaggtacgc
     3361 ggtctgctgg acggcggcgt cgacctgctg ctcgtcgaaa cgatcttcga cacgctgaat
     3421 gcgaaggcgg ctttttttgc gattcagcaa ctcttcgcag aaggtgcccg gcaggtcccg
     3481 atcatggcgt cggtcacgtt tatccaagcc gggagcaatc gcggcttctc gggacagacg
     3541 gttgaagggt tctggaattc tatttcgcat gtgccgctgc tgagtgtggg catgaactgc
     3601 gcgctgggcc cgaaggaaat gcggcccttg atcgaagagc tgtcgcagct cgcgccgatt
     3661 tttgtcagca gccatccgaa tgccggcttg cccaatccgt tgttaccgac gggctttccc
     3721 gaaaccccgg agtcgctggc gcctcagctg cgcgagtggg cacaaaacgg gtggcttaac
     3781 attgtgggcg gctgttgcgg gaccacgccc gaccatattc acactattgc ggaagcggtg
     3841 cgcggggtgt caccccgtcg gccggccaag gtcgagccgt ttttacgcct cagtggcctg
     3901 gaagccctga ccgtgcggcc ggaatcaaac ttcgtgaatg tcggcgagcg taccaac
//
LOCUS       DAOQEW010000294         3931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10295_length_3931_cov_6.958978, whole genome shotgun sequence.
ACCESSION   DAOQEW010000294 DAOQEW010000000
VERSION     DAOQEW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10295_length_3931_cov_6.958978"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLS35_18835"
     CDS             <1..811
                     /locus_tag="PLS35_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20784.1"
                     /translation="LSAQLTAREEDGLIGTVQSVETIDSLVSQTDYYDPQGRILERIQ
                     GGKDTSHGLWPLRFTYVYDKEGRRIAEMIRDAQGELVKETRAVYDAQGNRSAELSAWG
                     DGTFENVSLYDYDELRRLRRGLHYNAVHVVNRNLYSYDGGGRLLQERFERNYRYDADG
                     HQMVTTDRFDVGHDVAIIYNDRGQVQQKTVSDLRGRRQGHSEFRYDERGSQIEERSYD
                     AAGRLMDRKTYRYEYDTVGNWITETFQWWELRDGEPRIKQSHVRERRITYH"
     gene            928..2397
                     /locus_tag="PLS35_18840"
     CDS             928..2397
                     /locus_tag="PLS35_18840"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HNI20785.1"
                     /translation="MVRYSNLWQCLVTVLLLCGAGQVECWAGDTPHASHAHGPLRQSP
                     DNPRYFADADNRIVYLTGSHTWENFQDVTQVFDYPAYLDRLTALHHNFMRLWVTESIH
                     SDGGWLMNPPPNEKDIAPLPYLRTGPGLALDGGPRLDLSRFNPAYFERLRARVKDAGQ
                     RGIYVSVMFFQGFSVVTPGGWKAHPLNGQNNVNGIDGDANGDELGLEVHRSPGVPVLQ
                     IQEAYVRHVLETLHDLDNVLYEVVNEATPESVSWQYHFISFIKRYERERGFMAHPVGM
                     TFFQLGEFGGGDNRTLFESEADWISPGGYTKYARNPPATDGRQVQVLDTDHIHGIGGD
                     QEYVWESFMRGYNPIYMDPFDAVHELTIGEPVLNEPQHERARVAMGQTRRWADRINLK
                     RVTPQSELASTGYCLADRGREYLIYIPASDSVAESGAEKPTRPVTVNLTGASGVFTAE
                     WMELETGRTKQADSLIGGSERMLTVPFPGSGLLYLRKSG"
     gene            2569..2946
                     /locus_tag="PLS35_18845"
     CDS             2569..2946
                     /locus_tag="PLS35_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20786.1"
                     /translation="MRKLTPLHGDFLAYCVDDWTGLWLLVKRIKPLHSSTDADKIQAH
                     ALEIIRDLIEAGYIMAGDVDFGLGKFRQWNLSVDKIIGRIEQEWIELGRDPTICEIVW
                     FVSTTEGDYAFASGQFPETPSTE"
     gene            complement(3006..>3931)
                     /locus_tag="PLS35_18850"
     CDS             complement(3006..>3931)
                     /locus_tag="PLS35_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI20787.1"
                     /translation="IFIDGKRVNDLTVQERNIGFVFQHYALFKHLTVFDNIAFGLKIK
                     KWNRQDIEQRVLELLSLMSLDGLGSRYPHQLSGGQRQRVAIARALAPRPSVLLMDEPF
                     GAVDAKVRQELREWLIRLHTDLNVTSLFVTHDQEEAMEVSGRIIVFSKGKLEQAGSPA
                     DVYEEPATEFVARFIGSMNIVEGIVRRNQVQVGSLEFPAPGFSDGQKLQVGFRPYYVK
                     VAEDATRYRQQAKLRHIYFLGVAYRLEIETADGLILRSRMNKEEFRRCNFTVGQAVSF
                     AVTHFRILPQEGAEASSEAKPGSPITGPLTP"
BASE COUNT          850 a         1018 c         1185 g          878 t
ORIGIN      
        1 cctctctgcc cagctcacgg ctcgagaaga agacggattg atcggtaccg ttcagtccgt
       61 agaaacgatc gacagtctcg tctcgcagac cgactactac gatcctcagg ggcgtattct
      121 cgaacgcatt cagggcggca aggatacatc tcacggctta tggccgctgc gatttaccta
      181 tgtctatgac aaggagggaa gacgtatcgc cgaaatgatc cgtgatgcgc aaggcgaact
      241 cgtgaaggag actcgcgcgg tttatgatgc gcagggcaac cgctctgccg agttgtcggc
      301 gtggggggat gggaccttcg aaaatgtgtc cttgtatgac tacgatgaat tgcgacggct
      361 caggcgtggc ctccattaca atgccgtgca cgtcgtgaat cggaacctct attcctatga
      421 tggaggtggg cgtctgttac aggagcgatt cgaacggaat tatcggtacg acgcggacgg
      481 acatcagatg gtcaccacgg atcgattcga cgtggggcac gacgtggcga tcatctataa
      541 cgatcgtgga caggtacagc aaaagaccgt gtcggatctg cgggggcgta ggcaagggca
      601 ttcggagttt cgctacgatg agcgaggtag tcagatcgaa gagcgcagct atgatgctgc
      661 cggccggctc atggatcgca agacataccg gtatgaatac gatacggtcg gcaactggat
      721 tacagagacc tttcaatggt gggaactgcg tgatggtgag cctcgaatca agcaatcgca
      781 cgtccgcgag cgtcgaatca cctaccacta acgcacttcg tccggcatgt tccggatgtc
      841 tcaggtccct ccacgaaaga ctgtaagcgc cgctcgtctc tgcggcgtaa attttcgacg
      901 cctcgtgtgt gatctcgacg gagggcgatg gtgcggtatt ccaacctgtg gcaatgtctc
      961 gtgactgtcc tgctgttatg cggcgccggc caagtagagt gttgggccgg ggatactcct
     1021 cacgcgtcgc acgcacacgg acccttgcga cagtctcccg acaatccacg atattttgcc
     1081 gatgctgaca accggatcgt ttacctcacc ggatcccata cctgggaaaa ttttcaggat
     1141 gtcacgcagg tcttcgacta tccggcctat ctcgatcgtc tgacggcgct gcatcacaat
     1201 ttcatgcgct tgtgggtcac tgaatcgatc cattcggacg gcgggtggct catgaatcct
     1261 ccccccaatg aaaaggatat cgccccgctt ccgtatctgc ggaccgggcc aggccttgct
     1321 ctggatggtg gcccacgatt ggatctgtcc cgcttcaatc cggcctactt cgaacggctt
     1381 cgagcgaggg tgaaggacgc ggggcagcgc ggtatctatg tttcggtgat gttctttcaa
     1441 ggcttcagcg tggtgacgcc gggagggtgg aaggcgcatc cgctgaatgg tcagaacaat
     1501 gtgaacggca tcgacggtga cgccaacggc gacgagctgg gtttggaggt tcatcgatcg
     1561 ccaggagttc cagtgctcca gattcaagaa gcctatgtgc ggcacgtgtt ggagacgctg
     1621 cacgatctgg acaatgtctt gtatgaggtg gtgaacgagg ccacgcccga gtcggtcagc
     1681 tggcaatacc atttcattag cttcatcaag cgctatgagc gtgagcgagg cttcatggcg
     1741 catccggtcg gcatgacgtt ttttcaacta ggagagtttg gcggcggtga caatcgcacc
     1801 ctgtttgaga gcgaggccga ctggatctct cccggcgggt ataccaaata tgcgcgaaat
     1861 cccccggcca cggatggacg tcaggtgcag gtgttggata cggatcatat ccacggcatc
     1921 ggcggtgatc aggaatacgt ctgggaaagt ttcatgcggg ggtataaccc tatctatatg
     1981 gatccctttg acgcggtcca tgagttaacc atcggcgagc cggtgctaaa cgaaccgcaa
     2041 catgagcgcg cgcgagtggc gatggggcag acgcgccggt gggcggacag gattaacttg
     2101 aagcgtgtaa ctccacaaag cgagctggct tcaaccggct actgcttggc tgatcggggg
     2161 cgtgagtatt tgatctatat tcctgccagc gactcggtgg ctgagtccgg cgctgaaaag
     2221 cctactcgac cggtgaccgt caatctgacg ggcgcaagcg gcgtcttcac cgcggagtgg
     2281 atggaactgg aaaccggacg cactaaacaa gcagattccc tcatcggcgg cagcgagcgc
     2341 atgcttaccg tgcctttccc tgggagcggg ctgctgtatc tccggaagtc aggataaaga
     2401 atttctcgtc acagcatcag agaagattgc gggaaaaggt atcgctgcat ttcagtggaa
     2461 ggacgatcgg atgtaatcta attccgcatg tgatagctca gcaatagatt tgagtccgaa
     2521 agttttttga agctcttgac cggttccatt ttgagtgaag ataaagtaat gcgtaaattg
     2581 actcctctgc acggcgactt tcttgcatat tgcgttgacg attggaccgg tctttggctc
     2641 ctagtaaaac ggatcaagcc ccttcactca agcactgacg ccgacaaaat tcaagctcat
     2701 gcccttgaga tcatacgtga cttaatagag gcaggctata tcatggcggg tgatgtggac
     2761 tttggcttag gaaaatttag gcaatggaat ctgtctgttg acaagattat cggccgaatt
     2821 gagcaagagt ggatcgagtt gggtcgcgat ccgacaatat gtgaaattgt gtggtttgtc
     2881 agcacgactg aaggggatta cgcctttgcc agtgggcagt tccctgagac gccttctact
     2941 gaataaccaa gatcaaggtg ggggtggaat cctctagttt gctccactag ctggctcggt
     3001 tcgggttatg gcgtcagcgg tcccgtgatg gggctgccgg gcttggcctc gctcgacgcc
     3061 tcagcgcctt cttgcggcag gatgcggaag tgggtgacgg caaacgaaac ggcttgcccc
     3121 accgtaaaat tgcagcggcg aaactcttct ttgttcatgc gtgagcggag aatcaatccg
     3181 tccgccgttt caatttccaa ccgatacgcg acaccgagaa aataaatgtg gcggagtttg
     3241 gcctgctgac ggtagcgggt cgcatcttcg gccaccttca cgtagtaggg gcggaagccg
     3301 acctgcaact tctggccgtc ggaaaatccc ggcgcgggga attcgagtga gcccacctgc
     3361 acctgattgc gccgcacgat gccttccacg atattcatcg acccgatgaa acgcgcgacg
     3421 aattccgtgg ctggttcttc gtagacatca gcgggcgatc cggcctgctc cagcttgcct
     3481 ttggagaaga cgatgatgcg gccggatact tccatcgctt cttcctgatc gtgagtgacg
     3541 aagaggctgg tgacgttcag atccgtatgc aggcggatga gccactcgcg caattcctgc
     3601 cggaccttcg catccaccgc gccgaacggt tcatccatta agagaaccga ggggcggggt
     3661 gccagcgccc gggcgatcgc gacgcgctgt cgttgcccgc cggaaagctg gtgcgggtag
     3721 cggctaccga gtccgtcgag gctcatcaat gagaggagct ccagtacacg ctgttcgata
     3781 tcctgccggt tccacttctt gatcttgagg ccgaaggcga tgttgtcgaa gaccgtcagg
     3841 tgcttgaaga gggcgtaatg ttggaacacg aagccgatgt tgcgttcctg cacggtgagg
     3901 tcgttgacgc gcttcccatc gatgaagatg t
//
LOCUS       DAOQEW010000295         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10314_length_3927_cov_8.731663, whole genome shotgun sequence.
ACCESSION   DAOQEW010000295 DAOQEW010000000
VERSION     DAOQEW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10314_length_3927_cov_8.731663"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLS35_18855"
     CDS             <1..369
                     /locus_tag="PLS35_18855"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNI20788.1"
                     /translation="EEGAAARMLLDQRLEALGADGTSVKGYGVTVSSHFEVARHIAEG
                     HGDVGIGVRSAAQIFGLDFVPLQQARYDLVLPKRYLSSHPGLSHLLDAIASRQFRTEV
                     EALGGYDMTETGKVRSLRVG"
     gene            488..1264
                     /gene="modA"
                     /locus_tag="PLS35_18860"
     CDS             488..1264
                     /gene="modA"
                     /locus_tag="PLS35_18860"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI20789.1"
                     /translation="MVFWCGAGQAHAAESFVIAASPSLKGLLERLGSGFEQAHPEVRV
                     KLYFDSGLGLRQTIAGMENSMVGQYFVGSGPINLVAPGGDEVITRLQVKYYVLPGTKR
                     SYAVDQLVLVVPESLVEAPGSLEAIAQGNARLAIAERTRTRLGAQTADMLRASGIEEA
                     FKGRLDMATDGRGVIDHVLSGQADVGIIFGDQAVDQQQRLRVVGVINKGYQPTVHSMA
                     MERYCPNRRLCEEFLTYIQSAEAQRLVRQAGYAVPAMSGP"
     gene            1339..2013
                     /locus_tag="PLS35_18865"
     CDS             1339..2013
                     /locus_tag="PLS35_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20790.1"
                     /translation="MMNSRLIRSSRRGTSVLALMLIWALAAAGCARLPYTTKTIHEDQ
                     RVVVAVQRDVQPQTYSHPVQLTSADLTSLLRGFSIREQQRVPLRWFAEEQPPKPLFRE
                     DDLHALAPYLAEGFQHVGPNDRVHFEVRNPGFNPAYGRDTIAGWMAVRDPYLYLTIEY
                     FHTQIPVRKADDYDTNYPLTPPTPKEYILYFEPGRFYASDEKRVPAVDFRQFLKSGEA
                     GATAPR"
     gene            2077..3804
                     /locus_tag="PLS35_18870"
     CDS             2077..3804
                     /locus_tag="PLS35_18870"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNI20791.1"
                     /translation="MRLREGLYIFRDQWSGHRVLLGIVVLLAACFFSAGVTEAADTGK
                     LVEKDGKYVFVESMDPSLKLLLERSVKSGMITQEEYEQVVKDSEERTQLLSPTFRGWY
                     DRGFNLSMNDNAFFLKIRFRSQLRYTQRFRNDAWRNPGEAKNFPELLGVFGDYRANRS
                     ENSASSFNVRRLRFYFMGHLFDPDFKYYIQMRAETAENAQSPGSLSLFDVNFTYTKLP
                     WANVQFGQYKVYFNRSQINSTASMQFAERALVQDAFTASGLDRRDIGITIMNDEELFP
                     VNYYFGVFNGAGPSFNRLGSFTSEEATVGCPGGQTSGNPFPSPAGCPANQRNLNANTR
                     IDVDRLMYVARLNWNILGRPGYGEGDLAYSETPQFAVGGGYSYNPAVNTSSDNAFVGL
                     DLANLNIRRQLAAFGNGRQLGLGIVDYSTWAADGVFKYRGFSLQGEFYFKNVIRHDKG
                     LPCMNAACTTTAPNNFGNAMGWYVQGGYYLIPRTVEVAARYAYWDPDTNSANDLIRQA
                     DLSLNWFLNGTYDHQIMVTYSNTQFGTGGYAIGRSAPLPPGSGTVPLDAVGGTLIENA
                     IRVQYQIFF"
BASE COUNT          817 a         1035 c         1198 g          877 t
ORIGIN      
        1 gaggagggtg ctgccgcccg aatgttgctg gatcagcggc ttgaagcgtt aggcgccgac
       61 ggcaccagtg tgaaggggta cggagtgacc gtcagctcgc actttgaggt cgcacggcat
      121 attgccgagg gccatgggga tgtcggaatc ggcgtgcggt ccgctgcgca gatcttcgga
      181 ctcgatttcg tgccgttgca gcaggcccgg tatgacctgg tcttgccgaa gcgatacctc
      241 tccagtcatc caggcctttc ccatctcctc gacgctatcg ccagccgcca gttcagaacc
      301 gaagtcgagg cgttgggcgg gtatgacatg acggagacgg ggaaggtacg tagcttgcgg
      361 gtcggataac gcgcctccaa gcagcaggcg gatccgactc tgtacgatgt cgttggaggg
      421 atggatgaaa gggcagtggc gcatgagtca tcgagtgcga gcttttctct ctacatggtg
      481 tttggccgtg gtgttctggt gcggagccgg acaggctcat gcggccgagt catttgtcat
      541 cgcggcttcg ccgagcctca aaggattact ggagcgattg ggcagcggtt ttgagcaagc
      601 gcatccggag gtgcgggtga agctgtattt cgattcggga ctcggtttgc gccagacgat
      661 cgccgggatg gaaaacagca tggtcgggca gtacttcgtc ggcagcgggc cgatcaatct
      721 cgtggcgccc ggtggtgatg aagtcatcac gcgcttgcag gtgaaatact atgtgttgcc
      781 cggtacgaag cgctcgtatg ccgtggacca gttggtgtta gtggtgcccg agtctctggt
      841 ggaggctcca gggtcgctcg aggcgatcgc gcagggaaac gcgcgtctag cgattgccga
      901 gcgaacgcga acgaggttgg gggcgcagac agccgatatg ttacgtgcgt caggcataga
      961 agaagcgttc aagggacggt tggatatggc gaccgatggg cgtggagtga tcgaccatgt
     1021 gttgagcggg caagccgacg tcggcatcat tttcggggat caggctgttg atcagcaaca
     1081 gcggttgagg gtcgtcggcg tcatcaacaa gggctaccag ccgacggtgc attccatggc
     1141 gatggaacgg tactgcccca atcgccgcct ttgtgaggaa ttcctgacct atatccagag
     1201 tgcggaggcg caacggctgg tgcgacaagc aggttatgcg gtgccggcga tgagcgggcc
     1261 gtaaaaaatt ttgttcatgg tgcaaggata catatattta atatttgtat taatgcaatg
     1321 tgatcgtgcg aaggaggcat gatgaatagc agattaatca ggagcagcag gcgagggacg
     1381 tctgttctcg cccttatgct catatgggcc ctggcagcgg caggctgtgc caggctgccc
     1441 tatacgacca aaacaattca tgaggatcag cgtgttgtgg tggcggttca gcgcgacgtg
     1501 caacctcaaa cgtattcgca tccagtgcaa ctgacgagtg ctgatctcac cagtctgttg
     1561 cgagggtttt caattcgtga acagcaacgc gtgccgttgc gctggtttgc tgaagagcaa
     1621 ccaccgaagc cgctgtttcg cgaagacgat ctgcacgcgc tcgcgccata tctcgcagag
     1681 ggatttcagc acgtcggccc caacgaccgg gtgcatttcg aggtgcgcaa ccccggcttc
     1741 aatccggcct acggacgaga cacgattgcc gggtggatgg ctgtgcgcga cccctacctg
     1801 tacctcacga tcgagtattt ccatacccag atcccggtga ggaaagcgga tgattacgac
     1861 accaactatc cgctcactcc gcccacgccg aaggaataca tcctctattt cgaaccgggc
     1921 cgcttttacg cgagcgacga gaagcgcgtg ccggcggtcg attttcggca attcttgaaa
     1981 tcgggtgaag ctggtgccac ggcgcctcgc tgacggtgga taggccgtca cacctccgat
     2041 cgttcaacga actcactgac agtgatggag ccatgtatga gactcaggga aggtctgtat
     2101 atttttcgtg atcaatggtc tggtcatcgt gtgttgctgg ggattgtcgt gctactggcg
     2161 gcttgtttct tctctgcggg ggtgactgag gcagccgata ccggcaagct ggtcgaaaaa
     2221 gacgggaagt atgtctttgt cgaaagcatg gacccgtcgc tcaaattgct gctcgaacga
     2281 tcagtcaaga gcggcatgat tacccaggag gaatacgagc aggttgtgaa ggattccgag
     2341 gagcggacgc agttgctgtc gcccaccttc cgcggctggt acgaccgcgg atttaatctc
     2401 tcgatgaatg acaatgcctt ttttctcaag atccgattcc gctctcaact ccggtatacc
     2461 caacgattcc gtaacgatgc ctggagaaat cccggcgagg ccaaaaactt tcccgaactc
     2521 ctgggcgtgt tcggtgacta tcgagccaat cggtcagaga atagtgcctc ttcattcaac
     2581 gtccggcggc tgcggtttta tttcatgggc catctcttcg acccggattt caaatactac
     2641 atccagatgc gggcggagac ggccgaaaat gcgcagtcac cgggctcgtt gtcgctgttt
     2701 gatgtcaatt ttacgtacac gaaattgcca tgggccaacg tgcagttcgg gcagtacaag
     2761 gtgtacttca accggtctca aatcaattcc acggcgtcga tgcagtttgc tgaacgagcc
     2821 ttggtgcagg atgcctttac cgcgagcggt ctcgatcggc gcgacatcgg tatcacgatc
     2881 atgaacgatg aagaattgtt ccccgtgaac tattacttcg gggtgttcaa cggagcgggt
     2941 ccaagtttca atcgactggg ctcgttcacg tctgaagaag ccacggtggg ctgccccggc
     3001 ggccagacga gtggcaatcc cttcccctct ccggcggggt gtcctgcaaa tcagcgcaat
     3061 ttgaatgcga atacgcgtat cgacgtggac cgactcatgt atgtagcgcg attgaactgg
     3121 aacattcttg gacgtcccgg atatggagaa ggcgatctcg cctattccga aacgccgcaa
     3181 ttcgccgtcg gtggcggata ctcctataat ccggcggtca ataccagttc cgacaacgcg
     3241 tttgtcgggc tcgatttggc caatctcaac atcaggcgcc aattggcggc gttcggcaac
     3301 ggccggcaac tcggccttgg cattgtggac tactccacct gggcggcgga cggcgtgttc
     3361 aaatatcgcg ggttctcact ccagggcgag ttctacttca aaaatgtcat tcgtcacgac
     3421 aagggattgc cctgtatgaa cgccgcctgt accacgacgg ccccgaataa cttcggcaat
     3481 gccatgggct ggtatgtgca aggcggatat tatttaatcc ctcggaccgt ggaagtggcg
     3541 gcccggtatg cgtactggga tcctgacacc aattccgcca acgacctcat caggcaagcc
     3601 gatctgtcgt tgaactggtt cttgaacgga acctatgacc atcaaatcat ggtgacttac
     3661 agcaatacgc agtttggtac ggggggatac gccatcggcc gaagtgcgcc gctgcctcct
     3721 ggatcgggga cggtcccgct ggatgccgtg ggggggacct tgattgaaaa tgccatacgc
     3781 gttcaatatc aaattttctt ctagcaggat gcggaaaaag tccgccagct gcgttctcgc
     3841 gtccctcaga ggctcaccgt accgcgagag tacgattcgc ctcttcgctt gctgcggcct
     3901 tgctggacgg cctttttgcg catcctg
//
LOCUS       DAOQEW010000296         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10365_length_3917_cov_6.063439, whole genome shotgun sequence.
ACCESSION   DAOQEW010000296 DAOQEW010000000
VERSION     DAOQEW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_3917_cov_6.063439"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1493)
                     /locus_tag="PLS35_18875"
     CDS             complement(381..1493)
                     /locus_tag="PLS35_18875"
                     /inference="COORDINATES: protein motif:HMM:NF025708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HNI20792.1"
                     /translation="MYVPIIKGKLNDLRAVGALSPSVRDVIKPLIEAMPVNRQKHSTE
                     EHIFKFCDYVKKHVPVGEVFVDFFGLLPDEILEDGTNAIIYGYRLLKSLGRTVTPIYG
                     LERNDDLWDELGKLAANFGKGFAFRLRRDDLVDYQIDETWGAILERTAQMGLGAQDVD
                     LVLDFDNVGDATVSEIADVVFTFLWHNERIRDYRSVIVAGSSALKTVTEVGKDDRKEI
                     TRTELHLWSALWRDMPDDFKPVYGDYGVIHPDFSDLGPNKYMNAKIRYTAGDKILYFR
                     GHGLLHPVKDYAQYHDLASLVRSDSRFRGRGYCIGDAYLDDCASRQGSHGSPATWVKA
                     DMNHHLTYVAHQVNRLISAFLLETSDERAEALLVTV"
     gene            complement(1505..2758)
                     /locus_tag="PLS35_18880"
     CDS             complement(1505..2758)
                     /locus_tag="PLS35_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20793.1"
                     /translation="MEYKVLRTEAEHTRALQEAESLVARDPHSGTAEAERLELLAVLI
                     EDYEKRAFTFDELNPLEVIEFRMAEQGLRQRDLVSMIGSRSRVSEVLAGKRPLTVQMI
                     RALSVGLGIPADVLIGAELTKTQGPAVTNRETIDWKQFPVREMERRGWFQGVRVTGAT
                     VQERLQSFLAQVLPKEPEAVLFRRRFQGIEIDEKSYYSTLAWTARVLIRAREAGASLP
                     KFDPAKITTETLRDLARLSWLDNGPRLAVEFLAKLGIVVVIEHRLRNAIFDGAALLST
                     SGRPVIGLTLRIDRIDYFWFTLLHEVAHVWKHLNESGSAFVDRIERIATSERAPDAKE
                     NEANRVARDALIKRAVWERSPARLAPSRESVQELADQLHIHPAIVAGRIQFETGKYNQ
                     FRELLGQGEVRKQFDDLLQDEGEEE"
     gene            complement(2761..3045)
                     /locus_tag="PLS35_18885"
     CDS             complement(2761..3045)
                     /locus_tag="PLS35_18885"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNI20794.1"
                     /translation="MRVIGLSLLNAFCREHPDCRKWIANWISDAKRSDWATTHDIKAR
                     YASASFLPENVVIFNVRGNEYRLVTQIVFNVGVVAVKWIGTHAEYSKREL"
     gene            complement(3497..>3917)
                     /locus_tag="PLS35_18890"
     CDS             complement(3497..>3917)
                     /locus_tag="PLS35_18890"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNI20795.1"
                     /translation="QAQTEIVSFEPEELFGTKLRALLQRRKNRDLFDLDLGLTQLSMD
                     LGKIIASFEHYLALEGTPISRAVAEQRMLEKFTRSLTEDIAPLLPSGVRFEENDAVDA
                     FGRIWKGLIVRIQGEPWKLSGQVIEELRRGRVPLLLQ"
BASE COUNT          848 a         1134 c         1060 g          875 t
ORIGIN      
        1 tgatccctgc gggtgcagtg cggtgcaagc gagcggttct ccgccaacga aatacgccca
       61 caggctacca aggatgggag ggttgtcaag gaatggaagc cgctctgtag ggtggcgata
      121 tatctgatgc ttaatgaagc acaatctggc tgtttcctgt tgcacgctgc gacagattca
      181 cgattcattg ttgcttttcg gaaatgcttg ctgagtgtct gcgcgtcggc ccatagcggc
      241 cgctggaggt ggctcgactg gatggccggt gtggtacgga ttgctatcgt tgacgggaaa
      301 cgcggctcga cggctggttg gttcggcggc gaactcaccc attcgccctc tgccttcata
      361 aatgaagtac tcctcgtttt ctacaccgtc acaagcaatg cttctgcacg ttcgtcagac
      421 gtttccagaa gaaaggcgga tataagccgg ttcacttggt gcgcaacgta cgtgaggtgg
      481 tgattcatgt cggctttcac ccaagtggcg ggcgatccat gcgagccctg gcggctggcg
      541 caatcgtcga gatacgcatc gccgatgcag tagccgcgcc cccggaaccg cgaatcggat
      601 cgaacaaggg aggcaagatc atggtattgg gcatagtctt ttaccgggtg aagcagtccg
      661 tgcccccgaa aatagagaat cttgtccccg gccgtgtacc gtatcttggc attcatgtac
      721 ttgttcgggc caagatcgct gaaatccggg tgaatcacac cgtagtcgcc atagacgggt
      781 ttgaaatcgt ccggcatgtc gcgccacagg gctgaccaga ggtgaagttc cgtcctggtt
      841 atctccttcc tgtcgtcctt tcccacctcg gtcaccgtct tgagggcaga tgatccggca
      901 acgatcacgc ttcggtagtc acggatgcgc tcgttgtgcc acaggaaggt aaacacgaca
      961 tcggcaattt cagaaaccgt agcatctccc acgttgtcga agtccagtac aagatcaacg
     1021 tcctgtgctc ctagccccat ctgcgcggtg cgttcaagaa ttgctcccca ggtttcatct
     1081 atctggtaat ccaccaggtc gtcgcgccgc aggcggaatg cgaacccctt tccaaagttc
     1141 gcagcaagtt tccctagttc atcccacagg tcatcatttc gttccaggcc ataaatcggg
     1201 gtaaccgtgc gccccagaga cttcaggagg cggtatccgt agatgattgc gttagtcccg
     1261 tcctcaagga tctcatcggg aagcaatccg aagaagtcaa cgaacacttc tccgaccggc
     1321 acatgcttct tgacgtaatc gcaaaacttg aaaatgtgtt cctcggtact gtgtttctgg
     1381 cgattaactg gcatagcctc gatgagtggc ttgataacgt cccgtacgct tgggctgagc
     1441 gcccctaccg cgcgaagatc attgagtttc cctttgatga tgggcacata cacaggaact
     1501 gaggtcattc ttcctccccc tcatcttgca gaagatcgtc gaactgtttg cggacttcgc
     1561 cttgtccaag taactcgcgg aattgattgt atttcccggt ttcgaactga atacgaccgg
     1621 cgacaatcgc cgggtgaata tggagttgat cggcgagttc ctgaaccgac tcgcgtgaag
     1681 gcgcgagccg tgctggactg cgttcccaaa cggccctttt gatgagggcg tcacgcgcta
     1741 ctcggtttgc ttcgttttct tttgcgtctg gagcgcgttc ggatgtcgcg atgcgttcaa
     1801 tcctgtcaac gaaggcactc ccagattcgt tcaggtgttt ccaaacgtgc gccacttcgt
     1861 gcaacagcgt aaaccaaaaa tagtcgatgc gatcgatacg gagcgtgaga ccaataaccg
     1921 gccgtccgct ggtggaaagc agcgctgcac catcaaaaat tgcattgcgg agtcggtgtt
     1981 caatgaccac gacgataccg agctttgcga ggaactcaac ggcgagacgg gggccattgt
     2041 cgagccaact aagccgggca agatcgcgga gcgtctcagt ggtaatcttg gccgggtcga
     2101 acttgggcag cgaggcacca gcttcacgcg cccgaatgag aacccgggct gtccaggcca
     2161 acgtcgagta gtacgacttc tcgtcaatct caatgccttg gaatcggcgc cggaacagta
     2221 ctgcttccgg ttccttggga aggacctggg caaggaaaga ctgaagccgc tcctgtaccg
     2281 ttgcaccggt gacgcggaca ccctgaaacc atccccggcg ctccatttcc ctcacgggaa
     2341 attgtttcca gtcgattgtt tctcggttgg tgacggcagg cccctgggtt ttcgtaagtt
     2401 cggctccgat gagcacatcc gccgggatac caaggccgac ggagagggcg cggatcatct
     2461 gcactgtgag cggtcgtttg ccggcgagta cttcggatac ccggctccgg ctgccaatca
     2521 tggatacgag atcgcgctga cgaagccctt gttcggccat cctgaactca atcacttcaa
     2581 gcggattaag ctcatcaaac gtgaacgcac gcttctcgta gtcctcaatg aggacagcaa
     2641 gcagctccag gcgctccgct tcggccgtac ccgaatgtgg gtcccgtgcg acgagagact
     2701 ccgcttcctg gagtgcccgg gtgtgttcgg cttccgtccg caaaaccttg tattccattt
     2761 ctataactct cgtttgctgt attcggcgtg tgtgccaatc cacttcacgg ccaccactcc
     2821 aacgttgaat actatctggg tcaccagccg gtattcattt ccccgcacgt taaaaatcac
     2881 gacgttctcc gggaggaaac tcgccgacgc gtatcgtgcc ttgatgtcgt gagtcgtcgc
     2941 ccagtcgctc cgtttcgcat cactgatcca gttggcgatc catttccggc agtcaggatg
     3001 ttccctacaa aacgcgttca acaaactaag gccaatcact ctcatgcagg atttgctcca
     3061 tagacatccg cccctccgat atgttccaga tatggaatca aaacaattgt tcccaaatat
     3121 gtaacagctg tcaacagacc aacaatcaac gaactgcacc gctgccgccc cgatcgtcca
     3181 ccccatggcc agcttcttgg cgagagtccg gctctggtcc aaaaatgcat cctagcctga
     3241 tgcaaacggg cgatgagcca atgccacgga cattggtagc gtagaggccg aagtgagagt
     3301 tgccggagcg acgcggccac cggcgcagtc gaagcatcaa cgacagcaaa ttccactaca
     3361 ccggccggtc agtcaggtcc ccaccatcag ccgcaaaggc cgataagaaa ccgatcacca
     3421 tgctcacttg aaaaattggc ttacctatcc agagccaaca ttcaatacaa tctcgcgagt
     3481 tcctcttgtg ccttgatcat tgaaggagaa gcggcaccct cccccggcgc aactcctcaa
     3541 tcacctggcc agacagcttc cagggctctc cctggatccg aacgataagc cctttccaaa
     3601 tcctgccaaa ggcatccacc gcatcgtttt cctcgaagcg aactccggaa gggagcagag
     3661 gtgcgatgtc ctcggtcagg ctgcgcgtga atttctccag catgcgttgc tcggcgacgg
     3721 cacgggagat cggggtgccc tccagggcca gatagtgctc gaaactggcg atgatcttgc
     3781 ccaggtccat ggatagctgt gtgaggccca agtccaggtc gaacaggtcc cggttcttgc
     3841 gccgttgcag cagggcacgc aacttggtcc cgaagagttc ctcgggctcg aaggacacga
     3901 tctcggtctg tgcctgg
//
LOCUS       DAOQEW010000297         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10519_length_3880_cov_8.653072, whole genome shotgun sequence.
ACCESSION   DAOQEW010000297 DAOQEW010000000
VERSION     DAOQEW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10519_length_3880_cov_8.653072"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1381)
                     /gene="thrC"
                     /locus_tag="PLS35_18895"
     CDS             complement(131..1381)
                     /gene="thrC"
                     /locus_tag="PLS35_18895"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNI20796.1"
                     /translation="MSKMKALVCRECGKEYPPKAIHVCEMCFGPLEVKYNYDEIKSTI
                     SRKKIEQGPNSMWRYIDLLPVESTSIIGPHAGLTPLVRAKNLGAHLGIDELYIKNDTV
                     NHPTLSFKDRVVSVALTRARELGFETVACASTGNLANSVAAHAAAAGMKCYVFIPADL
                     EAAKVLGNLIYKPNVVEVEGNYDDVNRLCSEIAGEHGWAFVNINIRPYYAEGSKTLAF
                     ETVEQLGWRTPDQAVIPMASGSLLTKIWKGLHEMKALGLVDEVRTKINGAQAEGCSPI
                     ATAFKAGRDFFKPVKPKTIAKSLAIGNPADGYYALKATAESKGAMDAVTDEEVVEGIK
                     LLAQTEGIFAETAGGVTIGVLCKLVKQGLIKKNDVTVAYITGNGLKTQEAVVDAVGRP
                     FRIQPSLVNFQKTFKMGKNSGGES"
     gene            complement(1395..2222)
                     /gene="moeB"
                     /locus_tag="PLS35_18900"
     CDS             complement(1395..2222)
                     /gene="moeB"
                     /locus_tag="PLS35_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNI20797.1"
                     /translation="MEFTETQINRYSRHILLPEVGGKGQKKIIQGKVLIVGAGGLGSP
                     AALYLAAAGIGTIGLIDSDVVDLSNLQRQVIHQTPDVGRPKVVSGKEKIQALNPDVNV
                     VMYEERLTAGNALKIMSGYDVVIDGVDNFPTKFLINDACYFAGKPLVHGGILRFDGRV
                     TTIIPKKSACYRCVFKKPPPEGLVASCQEAGVIGVLAGIIGTIQATEALKLILGIGRP
                     LTDRLLDFDARRTQFREIRIKRNPDCPLCGERPTITELIEDGDVGGPTCALPGQRNL"
     gene            complement(2374..3303)
                     /gene="cysK"
                     /locus_tag="PLS35_18905"
     CDS             complement(2374..3303)
                     /gene="cysK"
                     /locus_tag="PLS35_18905"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNI20798.1"
                     /translation="MTAKAHGDITELIGGTPLVRLNRLTKPGSATIYAKVESFNPGGS
                     IKDRICLNMINEAERQGKLKPGGTIVEPTSGNTGIGLALVAAVRGYKLILVMPESMSM
                     ERASLLSSYGAQLVLTAAWEGMKGSIKEAESIVAQNPSYYMPDQFSNPANPAMHRKTT
                     GPEIVEALDGRVDAFVAAVGTGGTITGCGEVIRERNPAAKIVAVEPAGSPVLSGGDPG
                     PHKIQGIGAGFVPKVLNRTLLDRVITVTDDEAYQTAKLLAKKEGLLVGISAGANVFAA
                     QKIAEELGPGKNVVTILCDTGERYISIEKYFNI"
     gene            complement(3300..3500)
                     /gene="thiS"
                     /locus_tag="PLS35_18910"
     CDS             complement(3300..3500)
                     /gene="thiS"
                     /locus_tag="PLS35_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNI20799.1"
                     /translation="MQVMINGKAEEIAGGTVLDLLKAKSIEPQMVAVEVNDTMLERTH
                     LETTQLKDGDHVEFLFYMGGGR"
BASE COUNT          727 a         1160 c         1075 g          918 t
ORIGIN      
        1 agccggttct tcaggcccgg ataggtggca tcgagcgatc cgatcatgtc cacaatgttg
       61 gctgccgcgg attcaacctc accctggccc ttcgtgaggg gacgcaaggg cgttggaata
      121 cgaaccttaa tcatgattca ccaccactgt tctttcccat tttgaatgtt ttttggaagt
      181 tcaccaggct cggctggatc cggaacggcc gccccacggc atccacgacc gcttcctgag
      241 tcttgaggcc gttacccgta atgtacgcga cggtgacgtc gttcttcttg atcagaccct
      301 gcttgacgag tttgcacaac acgccgatcg tcacgccgcc tgccgtttca gcgaaaatgc
      361 cttcggtctg cgccagtaac ttgatccctt ctaccacttc ttcgtccgtc accgcgtcca
      421 ttgcgccttt gctttcggcg gtagccttca gtgcgtagta gccgtcagcc gggttgccga
      481 tggcgaggga tttggcgatg gtctttggct tcaccggctt gaagaagtcg cggccagcct
      541 tgaaagccgt ggcgatgggg gaacaccctt cggcctgcgc gccgttgatc ttggtacgca
      601 cctcatcgac caggcccagt gccttcattt cgtgcaggcc cttccagatc tttgtcagca
      661 gggagcccga ggccatggga atcacagcct gatccggcgt ccgccagccg agttgctcca
      721 ccgtctcgaa ggccaacgtt ttcgaccctt cggcatagta gggacgaata ttgatgttga
      781 cgaaggccca gccatgttca ccggcgattt cgctgcagag ccggttgaca tcatcgtagt
      841 tgccctcgac ctcgaccacg ttcggcttat aaatcaggtt gccgagaacc ttggcggctt
      901 cgaggtcggc agggatgaag acataacact tcatgcccgc tgcggcggca tgagccgcga
      961 cggagttggc cagattgcct gtggaggcgc aggctaccgt ctcaaaaccg agttcccgtg
     1021 cgcgcgtgag ggcgaccgag acgacccgat ctttgaatga cagggtcgga tgattgaccg
     1081 tatcgttctt gatgtagagc tcgtcgatgc ccagatgtgc gccgagattc ttggcccgca
     1141 ccaagggtgt cagtcccgca tggggcccga tgatcgaggt gctttcgacc ggtaacaggt
     1201 cgatgtagcg ccacatgctg ttcgggccct gctcgatttt cttgcgcgag atggtgctct
     1261 tgatctcatc gtagttgtat ttcacttcca gcgggccgaa gcacatctca cagacatgga
     1321 tggctttggg tggatattcc ttcccacatt cccggcacac gagcgctttc attttcgaca
     1381 tggagacacc ttcgctacaa gttacgttga cccggaagcg cacatgtcgg cccaccgacg
     1441 tcgccgtctt caatcagctc cgtaatcgtg ggccgctccc cgcatagcgg gcaatcgggg
     1501 ttccgcttga tgcggatttc tctgaactgc gtgcgtcgcg catcaaaatc cagcagccga
     1561 tcggtcagcg ggcggccgat gcccaggatc agcttgagcg cttcagttgc ttgaatcgtt
     1621 ccgataatac cggccaacac gccgatgacg cctgcttcct ggcaggaggc gacgagtcct
     1681 tcgggcggtg gcttcttaaa gacgcatcga taacaggccg actttttcgg aatgatggtg
     1741 gtcacgcgcc cgtcgaaccg gagaatgccg ccgtgcacca gcggtttgcc ggcgaaatag
     1801 caggcatcgt tgatgagaaa cttcgtcggg aaattatcga cgccgtcgat gaccacatcg
     1861 taaccgctca tgatcttgag ggcgttgcct gccgtgagcc gctcttcgta catcaccacg
     1921 ttcacgtcgg gattcagcgc ttggatcttc tctttgccgg agacgacttt ggggcgcccg
     1981 acgtccggtg tctggtgaat gacctgacgc tgtagattgc tgagatccac cacatcgcta
     2041 tcgatcagcc cgatcgtgcc gatgccggcg gcagccagat ataaggctgc cggcgaacca
     2101 agaccaccag ccccgacgat gagaaccttg ccctggatga tcttcttttg gcccttgccg
     2161 ccgacttcag gcaacaggat gtggcggctg tagcggttga tttgtgtttc tgtaaattcc
     2221 atgtccgacg gaagctgaaa accgtcacca gcggcgttct cgcatcattc aggccctcaa
     2281 cgtaccgcaa gggtacgcct cggcccttca ttcgctgcgg ccttgctgga tgacgctttt
     2341 gagcctcctc tccctaaatg gtctgccgta cgctcaaata ttgaaatact tttctatact
     2401 gatataccgc tcgccggtgt cgcaaagaat cgtgacgaca tttttgccgg gacccaattc
     2461 ctcagcgatc ttctgggcgg cgaagacgtt ggcgccggcg gaaataccga ccagcagccc
     2521 ttctttcttc gcgagcaatt tcgccgtttg ataggcttca tcgtcggtca cggtaatcac
     2581 gcggtcgagc agggttcggt tcaacacttt tggcacgaac cccgcaccga tgccttgaat
     2641 tttatgcggt cccggatctc cgcccgacaa cacgggggaa ccggccggtt ccaccgccac
     2701 gattttcgcc gccggattcc gttccctgat cacttcaccg cagccggtga tggttccacc
     2761 ggtcccgaca gccgccacga acgcatcgac ccgaccatcc agcgcctcca caatttccgg
     2821 accggtggtt ttacgatgca tggctggatt cgcgggattg gagaactggt cgggcatata
     2881 gtacgacgga ttctgcgcga cgatgctttc cgcttccttg atagagccct tcatcccttc
     2941 ccaggccgcc gtgagaacca gttgtgcgcc ataggaggac aacaggctcg cacgttccat
     3001 gctcatgctt tccggcatga ctaaaatcaa tttataccca cgaaccgcgg ccaccaatgc
     3061 gaggccgatg ccggtgttgc cgctcgtcgg ttcgacaatg gtcccgcctg gcttcagttt
     3121 gccctgccgc tccgcctcgt tgatcatgtt gaggcaaatg cgatccttga tgctcccgcc
     3181 ggggttgaac gattcgacct tggcgtagat cgttgccgag cctggtttgg tcaaccgatt
     3241 gagccgcacc agcggggtgc cgccgatcaa ctcggtgatg tctccgtggg ccttcgcggt
     3301 caccggccac cacccatgta gaagagaaat tccacatgat cgccgtcttt cagctgggtg
     3361 gtctcgagat gtgtccgttc cagcatggtg tcgttcactt caaccgcaac catttgcggt
     3421 tcgatgctct ttgctttcag caggtcgagg accgtgccgc ctgcgatctc ttccgctttg
     3481 ccgttgatca tcacctgcat atctgctcct gagatggtaa gagtctgaca ggatactgaa
     3541 catgtccgcc agcggttctc gcatctctca ggtcctcaac gtaccgcgag ggtacgcttc
     3601 gtcccttcgt ttgctgcggc cttgctggaa ggccatgttg agtatcctgt cgaaagggat
     3661 tctggttgcg ccttacacgc cgttgctcgg tgttcaatga gctcgacgtg aattgcagca
     3721 aactgctaaa gaatttcaag aagttagcaa acgcgttctt gtgttgtcaa ggcaacggac
     3781 ccccaacggg tgcgacgagg aagttgctgc gaagtcgtct ggtagagacg gccgctttct
     3841 tgactttcac catgcctagc ttacagtatg cgccggtctg
//
LOCUS       DAOQEW010000298         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10593_length_3863_cov_9.203256, whole genome shotgun sequence.
ACCESSION   DAOQEW010000298 DAOQEW010000000
VERSION     DAOQEW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_3863_cov_9.203256"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PLS35_18915"
     CDS             <1..1094
                     /locus_tag="PLS35_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HNI20800.1"
                     /translation="IALSNRSMWQTGGLMGAIVAIILVVMYLLLRNIVLQPMAKMSEI
                     SRDIAKGEGDLTKRVPAEGHDEIAQMGQYFNEFIEKLQHMIKKVAHVTDKVASASVEL
                     SATAEEISKGTDTLTSRASQTAAAVEEMNATVGQVAQNSGKAASLAQDTVKTAQEGGT
                     VVSSTISGMQQLSEAVSNSATIISDLGKSSDQIGEIVRTIEDIADQTNLLALNAAIEA
                     ARAGEQGRGFAVVADEVRKLAERTTKATKEIGDMIRQIQQDTRGAVDSMQQGTQKVTA
                     GVDLVNKTGEALSQIVRMVSESADMIRQIAVASEEQSVATQQIASDIENVAKVTKESS
                     SGAHESAKASQDLSQLAVELQGIVGGFKL"
     gene            1122..1640
                     /locus_tag="PLS35_18920"
     CDS             1122..1640
                     /locus_tag="PLS35_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNI20801.1"
                     /translation="MSATEQQVQSHIQAAAENSADRLVEKSADDLLQFVICLIGSEEF
                     AVDVLSVQEINRIVEVTRVPKTPPYVEGVINLRGRIIPVLDLRKLFGLTGAQQTTQTR
                     IVVVSVQARLVGLIVDSVEEVLRVPKSAIEPPPSVGTMAGAEFTQGVGRIDDRLLILV
                     DLSRLLLAREAA"
     gene            complement(1694..1936)
                     /locus_tag="PLS35_18925"
     CDS             complement(1694..1936)
                     /locus_tag="PLS35_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20802.1"
                     /translation="MGPADSLILDAKQAILDEQHRKFQVLQKEGRWTEAMQQFHVTLN
                     CASDVLAESIQLLERVLDARNRRGPSLPDSPDVPQS"
     gene            2067..2837
                     /locus_tag="PLS35_18930"
     CDS             2067..2837
                     /locus_tag="PLS35_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNI20803.1"
                     /translation="MDIATIIGIVVALGSIIGGQALEGGHLGSILQLTAFIIVAGGTF
                     GACCVQNPLSVVIKAATGATLVITNPPHDVKGTITQILDLANVARKQGLLALEGKLKD
                     LHDPFFKKGIQLIVDGTDPKLLQEILEIEVEHHEEEGVHAAKVWEAAGGYAPTVGILG
                     AVLGLIHVMENLADPSKLGGGIAVAFVATVYGVGLANLFFLPIANKMKFKLKEEAGLR
                     TMVIMGLVGLAQGENPRLLQEKLESFLPPHERTKEDKK"
     gene            2921..3694
                     /locus_tag="PLS35_18935"
     CDS             2921..3694
                     /locus_tag="PLS35_18935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNI20804.1"
                     /translation="MAKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKY
                     RTVSDSIKAALNPVNSTPSSRLPFAVGDSKPKMINTDMSSPNDPVIRRMKEVIKKIHP
                     SLALEMPDIKIIETGNGTIMISLPESILFGSGEARVRPEALPFLKSLAQILIEMDRHI
                     RVLGHTDNVPIRTAQFPSNWELSAVRAVMVVRIFSELNEVPISHLSATGFADSKPIAT
                     NDTPEGRTKNRRVEIIVLEKNLTEESLDSVLPNQGPPAL"
BASE COUNT          882 a         1088 c         1157 g          736 t
ORIGIN      
        1 cgatcgcatt gtcgaaccgc tccatgtggc agacgggcgg cctgatgggc gccatcgtcg
       61 ccatcattct cgtcgtcatg tatctgttgc tgcgcaacat cgtcttgcag ccgatggcca
      121 agatgagcga gatctcccgc gacattgcca agggcgaagg cgatctcacg aagcgggtgc
      181 cggccgaagg ccatgacgaa atcgcgcaga tgggccagta cttcaatgaa ttcatcgaga
      241 agttgcagca catgatcaag aaggtggctc acgtgacgga taaggtcgcg tccgcctctg
      301 tcgagctgtc ggccaccgcc gaggagatct cgaagggcac cgataccttg acgtcccgtg
      361 cgtcgcagac agccgccgcg gtggaagaaa tgaatgccac ggtcggacag gttgcgcaga
      421 actccggcaa agccgcgagc ctggcgcagg atacggtcaa gaccgcccag gagggtggaa
      481 cggtcgtatc gagcaccatc tccgggatgc agcagttgtc cgaggcggtg tcgaactcgg
      541 cgaccatcat ctctgatctc ggcaagtcgt ccgatcaaat cggcgaaatt gtccgcacga
      601 tcgaagacat cgccgaccaa accaacttgc tggccttgaa cgccgcaatc gaagcggctc
      661 gagccggcga acagggtcgt ggattcgccg tcgtggccga tgaagtccgg aagctcgcag
      721 agcgaacgac caaagccacg aaggaaatcg gcgatatgat ccgccagatc caacaggata
      781 cgcggggcgc cgtcgattcg atgcagcagg gcacgcagaa agtcacggcg ggtgtggacc
      841 tggtcaacaa gaccggcgaa gccctctccc agatcgtgcg gatggtgtcg gaaagcgccg
      901 atatgatccg tcagattgcc gtggcgtccg aagagcaatc ggttgcgacg caacagattg
      961 ccagcgatat cgaaaacgtg gcgaaggtca cgaaagagtc ttcgtccggc gcacatgagt
     1021 cggcaaaggc cagtcaggac ctcagccagt tggcggtcga attgcagggg atcgtgggtg
     1081 gattcaagct gtaagcagcc aaccacgaga aggagcccag catgagtgcg actgaacaac
     1141 aagtgcaatc tcacatccag gcggccgcag aaaattctgc ggaccgtctg gtcgagaagt
     1201 ccgccgacga tctgctgcag ttcgtgatct gcctgatcgg cagtgaagaa ttcgcggtgg
     1261 atgtcttgag cgtgcaggag atcaaccgga tcgtggaagt aacgcgagtg cccaaaaccc
     1321 ctccctatgt ggaaggggtg atcaatctgc ggggccgcat cattcccgtt ctggatcttc
     1381 gtaaactctt cggcctcacc ggggcacagc agaccactca gacccgcatc gtggtcgtat
     1441 cggtacaggc gcgattggtc gggctgatcg tcgattcagt ggaagaggtc ctgcgtgtgc
     1501 cgaaaagtgc gattgaacca ccgccctcgg tcggtaccat ggccggggcg gagtttacgc
     1561 agggagtcgg ccggattgat gatcggctgc tgatcctcgt ggatctgagc cggcttctct
     1621 tggcccggga agcagcctaa gccggaagga tgggcagtga aggcctgcct tctgcatggg
     1681 gagtgagcac gaatcaggac tggggaacat cgggcgaatc gggtagggag ggtcctcggc
     1741 gattccgtgc gtcgaggacc cgttccagca actgaatcga ctccgccaga acgtccgacg
     1801 cgcagtttag ggtgacatga aactgttgca tggcttccgt ccaacggcct tccttctgca
     1861 agacctgaaa cttgcggtgt tgttcatcga ggatggcctg tttggcatcg aggatcaagc
     1921 tatcggcagg gcccacaaga acacctccat ggaaaacgtg tgtgattcgg ttgcacgcta
     1981 tcaaagcggt tcccgggggc gcaagcccac cattctaggt agatggcgaa ggccacagtg
     2041 cgctgggacg gtgaggtaac tgaactgtgg atatcgccac aatcataggt attgtcgtag
     2101 ccctcggctc gatcatcggt ggacaagcac tcgaaggggg ccatctcgga tccatcctcc
     2161 aattgacggc gtttatcatc gtcgccggag ggaccttcgg cgcctgttgc gtgcagaacc
     2221 cgctgtctgt tgtgatcaag gcggcgaccg gggccaccct cgtcattacc aacccaccgc
     2281 acgatgtgaa gggcacgatc acgcaaattc tcgatcttgc caacgtcgcg cgcaaacaag
     2341 gcttgctcgc actggaaggc aaactcaaag atcttcacga cccgtttttc aagaaaggga
     2401 tccagctgat cgtcgacggc acagatccca agttgctgca ggaaatcctg gaaatcgaag
     2461 tcgagcatca tgaagaagaa ggcgtccacg cggcgaaggt ctgggaagcc gcgggtggat
     2521 acgcgccgac cgtcggtatt ttgggcgccg tgctcggttt gattcacgtc atggagaacc
     2581 tcgccgatcc atccaaactc ggcggtggta tcgcggtggc attcgtggcc acggtatatg
     2641 gcgtcggact cgccaacctg ttttttctcc ccatcgccaa caagatgaag ttcaaactca
     2701 aagaggaagc cggcctccgc acgatggtga tcatggggct ggtcggtctg gctcagggcg
     2761 aaaacccccg ccttctacaa gagaaactcg aaagcttcct ccctccgcac gagcgcacca
     2821 aggaagacaa gaagtgatgc gtcaccccgc cagttgccgc cgggacagat gtacaccgag
     2881 cgtctgcatg agtgccgcat agcccgcacg ggttgaagcc atggcgaaga aaaaacacga
     2941 agaacatgaa aatcacgaac gctggctggt gtcctacgcg gacttcatca cgctgctgtt
     3001 cgccttcttc gtggtcatgt attcggtgtc gtctgtgaac gaaggcaaat atcgaacggt
     3061 cagtgattcg atcaaggcgg cgttgaatcc ggtgaatagc acgccctcca gccggctgcc
     3121 cttcgccgtc ggcgactcca agcccaagat gatcaatacc gacatgtcga gcccgaacga
     3181 tccggttatt cggcggatga aggaggtcat caaaaagatt catccgtcgc ttgcgctgga
     3241 aatgccggac atcaaaatca tcgagaccgg aaacgggacc atcatgatct ctctgccgga
     3301 atccattctc ttcggcagcg gggaagcgcg ggtccgcccc gaagcgcttc cgttcctgaa
     3361 gtccctggcc cagatcctca ttgaaatgga tcggcacatc cgggtactcg gccatacgga
     3421 caatgtgccg atccggaccg ctcagtttcc ttccaattgg gaactctcgg ccgtgcgggc
     3481 ggtcatggtg gtccggatct tctccgaact gaatgaagtc cccatctcgc atctctcagc
     3541 cacgggcttt gccgactcta aacccatcgc caccaacgac acccctgaag gccggacgaa
     3601 gaatagacgg gtagaaatca ttgtgctcga gaaaaacctg acggaggagt ccctcgattc
     3661 ggtcctgccg aatcagggtc ctccggctct atgaccatgg tccaaaggcc tcagtcgact
     3721 ccagtgctaa atacaacccc ttacgtgtaa aggtttttgg gagacacacc gatacagagt
     3781 cacgtgaggt agcgaccgcc tctcgaaagg aacgagtgta tgacgctcaa gccggttggt
     3841 gatctgacga tttttgaggt ggg
//
LOCUS       DAOQEW010000299         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10712_length_3838_cov_8.516257, whole genome shotgun sequence.
ACCESSION   DAOQEW010000299 DAOQEW010000000
VERSION     DAOQEW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10712_length_3838_cov_8.516257"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="plsX"
                     /locus_tag="PLS35_18940"
     CDS             <1..1032
                     /gene="plsX"
                     /locus_tag="PLS35_18940"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNI20805.1"
                     /translation="MKIAVDAMGGDHGPAPVIEGAMQAAQELGVGIILVGKEDELTAA
                     CRKLNCNDTRITIRHAPQVVEMHESPAAVARKKRDSSIWVATELVKSGEADAVVSPGN
                     TGASMVASFFVLGLIKGVERPAIATMLPTLTGSAIMLDVGANVDCSAQHLEQFALMGN
                     EFARHVYAKPNPRVGLLSIGEEDSKGNEVTKEAFKLLKTSPLNFIGNIEGREVYSGSA
                     DVVVCDGFIGNVALKISEGVADVIKKLLMKELSGSWLGRLAYPLIAKPLLNLKRKIDY
                     AEFGGAPLLGVNGTTMICHGRSSAKAIKNAIRRAKGLAETRLDELIQRDIEESLRRHQ
                     DERLEDKQA"
     gene            1029..2006
                     /locus_tag="PLS35_18945"
     CDS             1029..2006
                     /locus_tag="PLS35_18945"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNI20806.1"
                     /translation="MIRGRITGTGSYAPERVMTNSDLEQLVATSDEWIRERTGIRERR
                     IAAPGQACSDLALIAAERALKAAGLSGSDLDLILLATCTGDMPLPSTACLLQHRLGAT
                     RAAACDISAACCGFVYALGVADAYVRTGMRHVLVVGSEVMSTITDWTDRNTCILFGDG
                     AGAAVVSAAEGERGILSSHLHSDGSLSDLIVVPGGGTRIPPSEAMVGERSQYIKMKGN
                     ETFKVAVRTLEEVARETLAAHQLSVDDLDLYIPHQANLRIIKAVADRLGLPLEKVVLN
                     MDRYGNTSAASIPIALDEAVRDGRVKDGQLVMFGAFGAGLTWASTLIRW"
     gene            2113..3066
                     /gene="fabD"
                     /locus_tag="PLS35_18950"
     CDS             2113..3066
                     /gene="fabD"
                     /locus_tag="PLS35_18950"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNI20807.1"
                     /translation="MTASAIGFLFPGQGSQSVGMGRGLYDAFPAVRAVYEEASTILGY
                     DAAQLCFEGPVERLNLTEHTQPALLVSSAAALKALEPAGLSPLAVAGHSLGEYSAVFA
                     AGGLSFRDAVALVQKRGRYMAEAVPPGTGLVAALLGLSAEVVKAVCQEASSVGVVAAA
                     NFNSPGQVVIAGEKVAVERAIDIAKSKGCKKAIPLPVSVPVHTPLMQGAADRLAAEFG
                     GVAWRDLTVPLINNAEAVALQRSEDIRASLVRQLPSSVRWEESVQTMAKMGVTTFLEI
                     GPGTVLTGLVKRILPGAVTANVHDQKSLDAALTTVGVNRQP"
     gene            3097..>3838
                     /gene="fabG"
                     /locus_tag="PLS35_18955"
     CDS             3097..>3838
                     /gene="fabG"
                     /locus_tag="PLS35_18955"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNI20808.1"
                     /translation="MSLQGKVAIVTGGAQGIGRAIAEGLAEDGADIAVADLDPARSQD
                     AVAAIQKLGRRALSVKVNVSDWNDAKAMADQVMQEWGRIDILVNNAGITRDGLLLRMK
                     EEDWNLVLQVNLNGTFHCTKAVLLPMTKQRFGRIVNIASIVGAMGNVGQANYSASKAA
                     VIGFTKTAAREYASRAVTVNAVAPGFIDTAMTQGLPPEVKEALQKQIPLGRLGLPSDI
                     AAAVRFLVSDAASYITGQVLHVNGGMLMV"
BASE COUNT          780 a          978 c         1291 g          789 t
ORIGIN      
        1 atgaagattg ccgtcgatgc gatgggcgga gaccacggac cggccccggt cattgaaggc
       61 gcgatgcaag ccgcgcagga actgggtgtc ggcatcattt tggtcggcaa ggaagacgag
      121 ctgaccgccg catgccgcaa actcaactgt aacgatacgc ggattaccat tcggcacgcg
      181 ccgcaagtgg tggagatgca cgagtcacct gccgctgtgg ccaggaagaa gcgggactcg
      241 tccatttggg tggcgacgga gctggtcaaa tccggtgaag ccgatgccgt ggtcagtcca
      301 ggcaataccg gcgcgagcat ggtggcctca ttcttcgtgt tgggattgat caaaggtgtc
      361 gagcggccgg cgattgccac gatgttaccg acattgacgg gaagtgcgat catgcttgat
      421 gtgggggcca atgtcgattg ttcggcgcaa catctggagc aattcgcctt gatgggcaat
      481 gaattcgcca ggcatgtcta tgccaaaccc aatccgcgcg taggactgtt gagcatcggc
      541 gaagaagaca gcaaggggaa cgaagttacg aaagaagcgt tcaagctgtt gaaaaccagc
      601 ccattgaatt ttatcgggaa tatcgaggga cgcgaagtct acagcggaag cgccgatgtg
      661 gtggtatgcg acgggtttat cggcaatgtg gccctcaaga tttcggaagg cgtcgcggac
      721 gtcatcaaga agttgttgat gaaggaactc tccggttcct ggctcgggcg gttggcttat
      781 ccgttgattg caaagccgtt gctgaatttg aagcgaaaaa tcgactatgc ggaattcggc
      841 ggcgcgccgt tgctgggggt gaatgggacg accatgatct gccatggccg ctcatccgcc
      901 aaggccatca aaaatgccat tcgacgagcg aagggcttgg ccgaaacccg gttagatgag
      961 ctgattcaac gcgacatcga ggagagcttg agacggcatc aggacgagcg gctggaggac
     1021 aaacaggcgt gatacggggg cggatcacag gaactgggtc ttatgcgccc gagcgggtga
     1081 tgaccaatag tgacctggag cagctggtgg cgacctcgga tgaatggatt cgcgagcgca
     1141 ccggaatccg ggaacggcgt atcgcggctc cgggacaggc ctgttcagat ttagcgttga
     1201 tcgccgcgga acgcgcatta aaagctgccg gactttccgg gagcgatctc gacctgattc
     1261 tcctggccac ctgcacgggc gacatgccgt tgccgtcgac tgcctgtctg cttcagcacc
     1321 gcctgggtgc gacgcgagcg gcagcctgcg acatctcggc cgcttgctgc gggttcgtct
     1381 atgcgttggg cgtcgccgat gcctatgtcc gaacggggat gcgccatgtc ctcgtcgtgg
     1441 gctcggaagt gatgtccacg attacggatt ggaccgatcg caatacctgt attttattcg
     1501 gcgatggcgc cggcgctgcg gtggtgagcg ctgccgaggg agaacgtggc atcctctcca
     1561 gccacctgca ttcggacggc agtttgtccg atctaatcgt ggtgcccggg ggcggaaccc
     1621 ggattcctcc ttctgaagcg atggtgggcg agcggtcgca gtacattaag atgaagggca
     1681 acgaaacgtt caaggtcgca gtacggactc tggaggaagt cgcacgcgaa acgctcgcag
     1741 cgcaccagct gtcagtcgat gatcttgatc tgtatattcc tcatcaggcg aacctgcgca
     1801 ttatcaaagc ggtggccgat cgtctcggtc ttccgctcga aaaagtcgtc ttgaatatgg
     1861 atcgatacgg caacacgtcg gcagcctcca tcccgatcgc cctggacgaa gccgtgcggg
     1921 acggacgggt caaggatgga caattggtga tgttcggagc tttcggcgcg gggctgacct
     1981 gggcgtcgac gctgattcgc tggtgaaggt gcggccactg gtcggtagcc ggatgtgaag
     2041 tccggcggtg ccgaatgtgt ccggcttttt ccgttgacat tgagcctggt ttctacgata
     2101 ccttacgcgg ttatgacagc ttcagccatc ggatttttgt tccccggaca ggggtcgcaa
     2161 tcagtaggca tgggacgcgg gttgtatgat gcgttcccgg cggtgagggc ggtgtatgaa
     2221 gaggcctcca ccatcctcgg ctacgatgcg gctcagctct gttttgaggg gccggttgaa
     2281 cggctcaatc tcacagaaca cacgcaaccg gccttgctcg tcagcagtgc agcagcgctg
     2341 aaggccttgg aaccggccgg actgagtccg ctggcggttg ccgggcatag tctcggggaa
     2401 tattcagcag tctttgcagc cggcgggcta tcgtttcgag atgcggtggc tctggtgcaa
     2461 aagcgtgggc ggtatatggc cgaagctgtt ccgccgggga cagggctggt ggccgcattg
     2521 ctcggtcttt ctgcggaagt ggtgaaagcc gtctgtcagg aggcctcctc tgtcggtgtg
     2581 gtcgccgcgg cgaatttcaa ttctcccggc caggtggtca ttgcgggcga gaaggttgcg
     2641 gtcgaacgag cgatcgacat tgccaaatcg aagggatgca agaaggcgat tccgcttccg
     2701 gtgagtgtac cggttcacac acccttgatg cagggggctg cagaccggct ggccgcggaa
     2761 ttcggcggag tcgcgtggcg ggatttgacg gtgccattga tcaataatgc cgaggcggtg
     2821 gccctgcagc gatcagaaga catccgggca tcgttggtgc gacagttgcc ctcgtccgtg
     2881 cgatgggaag agtcggtaca gaccatggcc aagatgggtg tgacgacctt tcttgaaatc
     2941 gggcccggga cggtcttgac gggcttggtc aagcggatac ttccgggtgc agtcaccgcc
     3001 aatgtccatg atcaaaagtc attggacgca gccctcacca ctgtgggcgt aaacaggcag
     3061 ccataagcag cttcgagatt gcgtgggagt agactgatgt cattacaggg aaaagtagcg
     3121 attgtcaccg gcggggccca gggaatcggg cgtgcgattg cggaagggtt ggctgaggat
     3181 ggcgcggata tcgccgtggc agacctcgac ccggcgcgct cgcaggatgc cgttgcggcg
     3241 attcagaaac tggggcgacg ggctctcagt gtcaaagtga atgtgtccga ctggaatgat
     3301 gcgaaggcca tggccgatca ggtcatgcag gaatggggtc ggatcgatat cctggtgaac
     3361 aacgccggga ttacgcgcga cgggttgctg cttcggatga aggaagaaga ctggaatctg
     3421 gtcctgcagg tcaatttgaa cgggacattc cattgcacca aagcggtgtt gctccccatg
     3481 acgaagcaac ggtttggccg tatcgtcaat atcgcctcga tcgtcggggc gatgggaaac
     3541 gtggggcagg cgaactattc ggcgtcaaaa gcagcggtca tcgggtttac aaaaacggcc
     3601 gcgcgtgaat atgccagtcg cgccgtgacg gtcaacgccg tggcgccggg ctttatcgat
     3661 acggctatga cgcaggggtt gcccccggaa gtgaaagaag ccttgcagaa acagatcccc
     3721 ctgggacgat tggggcttcc ttccgatatt gccgcggcgg ttcggttttt ggtgtcggat
     3781 gcagcgtcgt acatcacggg gcaggtgttg cacgtgaacg gcggcatgct catggtat
//
LOCUS       DAOQEW010000300         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10713_length_3838_cov_6.436426, whole genome shotgun sequence.
ACCESSION   DAOQEW010000300 DAOQEW010000000
VERSION     DAOQEW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_3838_cov_6.436426"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLS35_18960"
     CDS             <1..456
                     /locus_tag="PLS35_18960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNI20809.1"
                     /translation="PLRHKAAVFMGDAGSMMLGALLAWFAIQLSNSGSTIPQPPPPFA
                     ILWMLGLPVLDTVVLMVRRIRQGRSPFSAGRDHMHHIWMHAGFTPGETTLLLMGLNGL
                     LGLIGFAGWHLGVSEWLLAAGYGSLFILHSTVATHAWKVSKWLKRQIYT"
     gene            1228..2526
                     /gene="wecC"
                     /locus_tag="PLS35_18965"
     CDS             1228..2526
                     /gene="wecC"
                     /locus_tag="PLS35_18965"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: protein motif:HMM:NF008286.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HNI20810.1"
                     /translation="MQLSFETISIIGLGYIGLPTAAVFASRKVKVIGVDLNQHAVDTI
                     NRGEIHIVEPDLDMMVRSTVSEGYLRATTQPEPADAFLIAVPTPFKEGSSGPHYPDMD
                     YIEAASKSIAPVLKKGDLVILESTSPVGTTEQMAAWLAKARPDLTFPQTHGEASDIRV
                     AHCPERVLPGHVLRELVQNDRVIGGMTLRCSEAAARLYQIFVQGECVITNARTAEMCK
                     LTENSYRDINIAFANELSIICDKLDIDVWELIHLANRHPRVNILQPGPGVGGHCIAVD
                     PWFIVSAAPEEARLIRAAREVNDGKPGWVLGKVKLAIADLLMAHPDRTVHDVSVACLG
                     LAFKPDIDDLRESPSAVIACRLAKLHPGMVIAVEPNIDELPAHWEAPLLLKPLDEALA
                     LADIVVILVDHQAFKALTPQTLAGKRCIDCKGLLRPQPKA"
     gene            2533..3591
                     /locus_tag="PLS35_18970"
     CDS             2533..3591
                     /locus_tag="PLS35_18970"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HNI20811.1"
                     /translation="MSNPGRQADILHLVEHEKFIPAFISLINQHLDTRRHHFFFVHNG
                     HRYRPQGAEIVSIDARQKSRLHQLWSLAQALNQADKIVLHGLFNPSLILLLFLQPGLL
                     PKCHWIIWGGDLYVHSFNRGDWRWRVKEWFRRPIIRRFGHLVTYVAGDVELARKWYGA
                     RGQYHECLVYPSNTYRPIELPAEHHDGLQILVGNSADPSNEHLEVLQILQAQSGTPIK
                     VFVPLSYGNAGYAQTVVEQGRRLLGDRFVPLRDFMPYDDYLRMLAGIDIAIFNHRRQQ
                     GMGNIIALLGMGKTVCIRSDTTSWQTLTGMGLRLCDTLNPDLRTLAPEDAASNQAIII
                     RRFSESVLIDQLRAFLEN"
     gene            3593..>3838
                     /locus_tag="PLS35_18975"
     CDS             3593..>3838
                     /locus_tag="PLS35_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20812.1"
                     /translation="MPYLTEAQLAQMGFRSIGKEVRISDKAAIYNADQIDIGDHSRID
                     DFCVISGRVRIGAYVHVAPHCLVAGGSEGITLEDFAGL"
BASE COUNT          843 a         1215 c         1029 g          751 t
ORIGIN      
        1 cccctgcgcc acaaggcggc tgttttcatg ggcgacgccg gaagcatgat gctaggcgca
       61 ctgctggcgt ggttcgcaat acagctttcg aatagtggca gcaccatccc ccagccgcca
      121 ccgccgttcg cgatcctgtg gatgctgggc ttgccagtac tcgataccgt cgtcctgatg
      181 gtgcgccgca tccgccaggg acgcagcccg ttcagcgccg ggcgtgatca catgcaccac
      241 atttggatgc atgcggggtt tacgccaggg gagacgacgt tgctactgat ggggctgaat
      301 gggctgctcg gcttgattgg ctttgcaggg tggcatctgg gtgtttccga atggttgctg
      361 gctgcgggct acgggagttt gttcatcctg cactcgacgg ttgcaacgca tgcgtggaag
      421 gtgtcgaaat ggctgaagcg ccagatctat acatagagac cgaatcggtc gtcgcatcaa
      481 cagcgtcctg acttggcgct cgatccggtc gctcatccgt tagggctgct tggctacgaa
      541 tgtcgaaatc gtgatcgacc caaagcctcc gtgggcacag agcatgcaca cgcagaacat
      601 gggttgcata aacttctgtc gcgtaggctc gcgatcagcg ctgaggccgt ggcaagggcg
      661 gggactagac atcaagcaaa aatgaataaa acagtcaagc tcctcaccgt ctttggcacc
      721 cggccagaaa ccatcaaaat ggggcccttg gccaaagaac tccaagcatt tccagatttt
      781 gaacaccgcg tgtgcgtcaa ggcccagcac cgacagatgt taggccaaat ccttcaactg
      841 ttggacatcc ccccactacg atctggatct tatgcctcct aagcagaccc tgccgcaatt
      901 gaccagcaac atcctgtata cgtgatgaag cgcagccaca tcatcctgac agactccggc
      961 ggcatacaag aagaagccct ttcactacgt aagcccgtgc tactgatgcg tgaaacaacc
     1021 aaacgtcccg aggccgtcgc tgctggcacc gtgcacctgg tgagaatcga cactacccgt
     1081 accgtgaaag cccccctcga gttgctgaac gatgaagccg tgtaccagcg catggctgtc
     1141 gcccacaacc catgtggcga cggagtcgca tttaaaagaa tccgtcaagc ctttgaatct
     1201 actagacagc catcctgacc caaccctatg caactctcgt ttgaaaccat ctcaatcatt
     1261 ggcttggggt acattggttt acccacggct gccgtatttg ccagccgcaa agtcaaggta
     1321 atcggcgtgg acttgaatca gcacgccgtg gacactatca accgcggcga aatccacatt
     1381 gtcgaacctg atctggacat gatggtgcgc tccaccgtca gcgaagggta cttgcgtgcc
     1441 acgacacagc ccgagccagc cgacgccttc ctgattgccg tgcctacgcc gttcaaggaa
     1501 ggctcttcgg gaccccatta tccagacatg gactacatcg aggcagccag caaatcaata
     1561 gcgcccgtgc taaagaaagg tgatctcgtc atcctcgagt ccacttcacc cgtgggtacc
     1621 accgagcaaa tggcggcttg gctggctaaa gcccggccag acctgacatt tccgcaaacc
     1681 catggtgagg ccagcgacat tcgcgtcgcc cattgtccag aacgtgtgct accgggtcac
     1741 gtcctacgcg aactcgtgca gaacgaccgc gtcatcggag gcatgacact ccggtgctcg
     1801 gaagcggcag cccgtcttta ccagatcttc gtgcagggcg agtgcgtcat cacaaacgca
     1861 cgcacggccg aaatgtgcaa gctgaccgaa aacagctacc gcgacataaa catcgcgttc
     1921 gccaacgagt tgtccatcat ctgcgacaag cttgacatcg atgtctggga actgatccat
     1981 ctggccaacc gccaccctcg cgtcaatatc ctgcaacccg gcccgggcgt cggcggccac
     2041 tgcattgcag tcgacccgtg gttcatcgta agcgccgctc cagaggaggc ccgactcata
     2101 cgtgccgccc gcgaggtcaa cgacggcaag cctggatggg ttctcggtaa ggtcaagctc
     2161 gccatcgcgg acctcctcat ggcacatccg gacagaactg ttcacgacgt gagcgtggcc
     2221 tgcctgggtc tggccttcaa gcccgacatt gacgatctgc gcgaaagtcc ttctgccgtc
     2281 attgcgtgcc ggctcgccaa gttgcacccg ggtatggtca tcgctgtcga accgaacatc
     2341 gatgaactac cagcacactg ggaagcgccc cttctgctca agcctctgga cgaagccctt
     2401 gccctggccg acatcgtggt catcctggtg gaccaccaag cctttaaagc gctgactccg
     2461 cagaccctgg cagggaaacg ctgcatcgac tgcaaagggc tgttgcgccc acagcccaag
     2521 gcctgaattc gcgtgagcaa tcccggccgc caagcagaca tcctgcatct cgtggaacac
     2581 gagaagttca ttcctgcctt catctccttg atcaaccagc acctagacac gcggcgccac
     2641 cattttttct ttgtacataa cggacaccga tataggccac aaggggccga gatcgtcagc
     2701 atcgatgcca ggcaaaaaag ccgcctacac cagctctggt cgctggcaca agcgctcaac
     2761 caagccgaca aaatcgttct gcacggcctc ttcaacccgt cactaatcct gctgctgttt
     2821 ctccaacccg ggctgcttcc caagtgccac tggatcatct ggggtggcga cctctacgtc
     2881 cactctttca accgtggcga ctggcgatgg cgtgtcaagg agtggttccg acggccgatc
     2941 attaggcgct tcggccatct cgtgacctac gttgccggcg acgtcgaact cgcccgaaag
     3001 tggtacgggg cccggggcca gtaccacgag tgtctcgttt accccagcaa cacctaccgt
     3061 ccgatcgagt tgcctgccga gcaccacgat ggtctgcaga tcctcgtcgg caactcggcc
     3121 gatccatcaa acgaacacct cgaagtgctg cagatattgc aggcgcaaag cggaaccccg
     3181 atcaaggtct ttgtgcccct ttcctatggc aacgcaggtt atgcgcagac cgtggtcgaa
     3241 caggggcgac gcctgctcgg cgaccgcttc gtcccgctgc gcgacttcat gccgtacgac
     3301 gactacctgc ggatgcttgc gggcattgac atcgcaatct tcaaccaccg gcgtcaacaa
     3361 ggcatgggaa acatcatcgc cctgcttggc atgggcaaaa cggtctgcat ccgctcggat
     3421 acgacctctt ggcagaccct caccgggatg ggcctgcgtc tgtgcgacac gctaaacccc
     3481 gatctccgca ccctcgcacc cgaggacgct gcgagcaacc aggccatcat catccggcgc
     3541 ttctccgaaa gcgttctgat cgaccaactt cgtgcattcc tggagaactg acatgcccta
     3601 tctgacagaa gcacaactcg cacaaatggg cttccgatcc attggaaaag aagtccgcat
     3661 cagcgataag gcggccatct acaacgcaga ccagatcgac atcggcgacc actcgaggat
     3721 cgacgacttc tgcgtgatct cggggcgcgt tcggataggg gcctacgttc acgtggcgcc
     3781 acactgcctt gtcgcaggtg gcagtgaagg catcacgctt gaggatttcg ccggactg
//
LOCUS       DAOQEW010000301         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_10898_length_3803_cov_7.663821, whole genome shotgun sequence.
ACCESSION   DAOQEW010000301 DAOQEW010000000
VERSION     DAOQEW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10898_length_3803_cov_7.663821"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLS35_18980"
     CDS             complement(<1..1235)
                     /locus_tag="PLS35_18980"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20813.1"
                     /translation="MTPRPPMKVLLLQLEFPTWKQARAWTYPACFGVAEGLRASGVDC
                     TTLPLIADPPYSSTAWLAHMKRMVKGQRFDQVWVWLVHAPLDEAILNWLSELAPIRVG
                     IIMESLQYSEEDYAWAPHLRARQGFVEQQVRALTHVLVPDECDVERTAARTGVPALWF
                     PPMVPERFISTPQNAPVHRVGVFHGVPYGPREQWIHHPALKSCLTFARPGAPTLFQQL
                     FDRLQQSAIQRLATPAGMPAAELTHYTTMLQEVREGEFREWMTQLPLWAAIVNLPSLA
                     KFFGGRVFEGIAAGRPVVSYAVPGHPMNNSLFVEGEEILYFSPSRPDSLAEVLDRLLA
                     DDTFATRIAGNAQHKLRTYHTSERRLVETLAWIQSGTTPVYGEREAGAAYMLSSAKTG
                     HAAPSVEPRPTQAIGHVDTT"
     gene            complement(1257..2882)
                     /locus_tag="PLS35_18985"
     CDS             complement(1257..2882)
                     /locus_tag="PLS35_18985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI20814.1"
                     /translation="MNVLLLYPTWTGAYGLFGHFARRNSTWPPLNLALLAAIAERHGH
                     NVTILDGESEQVPLEEMVQRAVAMKPDLIGFTATSPFFHLSKTVAEGIKRLAPEIPIA
                     VGGPHITIMKEQALLGCFDYAFIGEAEESWPQFLNAYEQRKDLSTVRGIIYRRNGELV
                     STGQPADITDLDALPIPARHRLPMSRYKLGTLRGRLPFTSIQTMRGCPWKCIFCASEA
                     LKTTEMRVRSPRSVVNEMKQVVEIFGTRHFYIVDDVMTLWKDHILQICDLIDQEGLQI
                     TFEGSTRANLVEEDVIARLVKSGLIRLSFGLETVDPEIRRTMKKQVPLEHYVKANGIC
                     NRYGVEALNSVMIGLPGETRETVRATLNFLRQAREVKQANFAIAVPYPGTEFHKLAVN
                     GEKGVRLMTQDFSEYRRYGSAVTTVGELTPHDLIDLQNEGFVSIYSAPWRWIPMLQKH
                     GMIGGSLMLVRVARLLSKKLFAGHGQSSQEQLISLGDGAFGRIQESAMSSQPVGVAPT
                     GPNSSAPLLTIASSSRAGAAPAGHFGHPTNPNG"
     gene            complement(2954..>3803)
                     /locus_tag="PLS35_18990"
     CDS             complement(2954..>3803)
                     /locus_tag="PLS35_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNI20815.1"
                     /translation="AKAFTQRVLIALDRRSKPVPDFSAWTGRVMEWKRRYDPVRPEFF
                     AQRERVHPYAFMRRLSEKMGATDVLVGDCGGNIVVSNHAFETKYGQRNLTNNGNSPMG
                     FSFAGAMGAWFADPTRQVVCTIGDGGFNMNPQELQTFVNYGVKVKTFILNNHIYGITK
                     AYQETNFQGRAEACGPKGYHPPDFLKVVQAYGIKTVAIANHAEMDAKIDEVLQFDGPV
                     VCDVDMHEFHTYEPRIFGWKTPIEDMYPYLPREEFRANMVIEPAEGWMSPEYPDVVRR
                     TDQSQP"
BASE COUNT          763 a         1119 c         1110 g          811 t
ORIGIN      
        1 gtcgtatcga cgtggccaat ggcttgtgtg gggcgcggtt cgacagatgg agccgcgtgg
       61 ccggtcttgg cagaggacag catgtaggcg gcgccagcct ctcgttcacc atacacaggc
      121 gtggttcccg actgtatcca ggcgagagtc tctacaagcc gtcgctcgct ggtgtgatag
      181 gtgcgcaatt tgtgttgggc gttgcctgct attcgcgtcg cgaatgtgtc atcggccaat
      241 agacggtcca gaacctccgc cagtgagtcg ggccggctcg gtgaaaaata gaggatctcc
      301 tccccttcca cgaacaaact gttgttcatc ggatgtcccg gcacggcgta cgacacgacc
      361 ggtcggccgg cggcgatccc ttcaaacacc cgaccaccaa aaaatttcgc cagactcggt
      421 agattgacaa tcgccgccca tagcggcaat tgagtcatcc actcccggaa ttccccttcg
      481 cgcacttcct gcaacatcgt cgtgtagtga gtgagctcag cggcgggcat tcccgccggt
      541 gtggccaacc gctgaatggc tgactgctgc agccggtcga acaattgttg aaacaacgtt
      601 ggcgcgcctg gccgggcaaa ggtgagacaa gatttcagcg cgggatgatg gatccactgt
      661 tcacgtggac cgtagggcac gccgtggaag acgccgactc gatgaacggg tgcgttctgt
      721 ggcgtactga taaagcgttc cggcaccatc ggcggaaacc agagagcagg aacgccggtg
      781 cgtgcggcgg ttcgctctac gtcgcattca tccggaacga gcacatgggt caatgcacgg
      841 acctgttgtt ccacgaaccc ctgccgtgcc cgaagatgag gcgcccatgc gtagtcctcc
      901 tcgctgtact gcaaggattc catgatgatg ccgacacgaa tcggggctaa ctcactcaac
      961 cagttcagaa tcgcctcatc gaggggcgca tgaaccagcc acacccacac ctgatcgaac
     1021 cgttgccctt tgaccatccg cttcatgtgc gccaaccagg ccgtactgga atagggcggg
     1081 tcggcgatga ggggaagcgt agtgcagtcc acgccacttg cccgtaaccc ttcagcgact
     1141 ccaaaacagg ccggatacgt ccaagcgcga gcttgtttcc aggtgggaaa ctccagctgc
     1201 aagagaagca ctttcatggg tggacgtggt gtcatgaggc ctttccgttg ggaccattag
     1261 ccattggggt ttgtgggatg accgaaatgc ccggcgggcg cggctcccgc ccgggatgac
     1321 gaggcgatcg tcaacaatgg agcgctgctg ttcggtcccg ttggagcgac tccaaccggc
     1381 tgtgagctca tggctgattc ctggatgcga ccgaacgctc catctcccag gctgatgagc
     1441 tgctcctgtg aggattgccc gtggccggca aacagttttt ttgacaggag gcgtgccaca
     1501 cggaccagca tgagtgagcc gccgatcatg ccgtgtttct gcagcatggg aatccagcgc
     1561 cagggcgcgg aataaatact gacgaatccc tcattctgca ggtcgatgag gtcatgaggc
     1621 gtcagctccc ccaccgtggt gacggccgat ccatagcgtc gatattcgga gaaatcctgc
     1681 gtcatcagcc gaacgccttt ttcgccgttg accgccagct tgtgaaactc ggtccccgga
     1741 tacggcacgg cgatggcaaa attcgcttgc ttgacttctc tggcctgccg taaaaagttc
     1801 agcgtcgctc gcacggtttc tcgtgtttcc cccggcaatc cgatcatcac ggagttcaag
     1861 gcttccactc cgtaccggtt gcagatgccg tttgctttca cgtagtgctc cagcgggacc
     1921 tgttttttca tcgtccgccg tatttcgggg tcgacggttt cgagtccgaa cgacagccgg
     1981 atgagcccgc tcttgaccag ccgggcgatc acgtcttctt ccacgagatt ggccctggtg
     2041 ctgccttcga aggtaatttg caacccttcc tgatcgatga ggtcacaaat ctgtagaatg
     2101 tggtctttcc agagcgtcat cacatcgtcg acaatataga agtgccgcgt gccgaagatc
     2161 tccacgactt gcttcatctc gttgacgacc gatcgcggag agcgcacacg catctcggtg
     2221 gtcttcaacg cttccgaagc gcagaaaata catttccacg ggcagccgcg catggtttga
     2281 atcgaagtga acgggagacg tccgcggagc gtgccgagct tgtaccggga catcggtaat
     2341 cgatgccgcg caggaatcgg caaggcgtcg agatccgtga tgtcagccgg ctgaccggtc
     2401 gagacgagtt ccccattccg ccgatatatg atcccgcgca ccgtcgacag gtccttgcgt
     2461 tgttcatacg cgttcaggaa ttgaggccaa gactcctcgg cctcgccgat gaaggcatag
     2521 tcgaagcagc ctaacaacgc ctgttctttc atgatggtga tatgagggcc tcccacggcg
     2581 atgggaatct cgggagccag gcgtttgatc ccttccgcta cggttttgct caaatggaaa
     2641 aaggggctcg tcgcggtaaa gccgatgaga tcgggcttca tcgccaccgc ccgctgcacc
     2701 atttcctcca ggggcacctg ctcggattcg ccgtcgagaa tcgtgacatt gtgcccgtgg
     2761 cgttcagcaa tggccgccag gagcgcgagg ttcagcggcg gccaggtgga attccgccgg
     2821 gcaaaatggc cgaacagtcc gtaggcaccg gtccatgtgg gatacagtaa caagacgttc
     2881 atacatcacc tcatgtaatc gatgagcgcc cttgcctcac taaccgggcc gccgcatcgt
     2941 gtcgctccac tcactacggc tgtgactgat ccgtccggcg caccacatcg ggatactcgg
     3001 gactcatcca tccttccgcc ggttcgatga ccatgttggc gcggaattct tctcgaggca
     3061 gatagggata catatcctcg atgggcgtct tccaaccgaa gattctcggt tcgtacgtat
     3121 ggaactcgtg catatccaca tcgcagacga ccggtccgtc aaactgcagc acctcgtcga
     3181 tcttggcatc catctcggca tggttcgcga tggcgacggt cttgatgcca taggcttgga
     3241 cgaccttgag gaaatccggc ggatggtagc ccttcggccc gcaggcttcg gcacgtccct
     3301 ggaaattggt ttcctggtag gccttggtaa tgccgtaaat atggttgttc agaatgaacg
     3361 ttttgacttt gacgccatag ttgacgaatg tctgcaactc ctgcgggttc atgttgaagc
     3421 cgccgtcgcc gatcgtgcag accacttgtc tcgttggatc ggcaaaccag gcgcccattg
     3481 ctcccgcaaa ggagaacccc atgggagaat tgccattgtt ggtcaggttg cgctgcccgt
     3541 acttggtctc gaaggcatgg ttgctgacga cgatattgcc gccgcagtcg cccaccagca
     3601 cgtccgtggc tcccatcttt tctgagagac ggcgcatgaa cgcatagggg tggacgcgct
     3661 cacgttgggc gaagaactcc ggacgaaccg gatcgtaccg ccgcttccat tccatgacgc
     3721 ggcccgtcca ggctgagaaa tccggcacgg gtttgctgcg tcgatcgagc gcgatcaaga
     3781 cacgctgtgt aaacgccttg gcg
//
LOCUS       DAOQEW010000302         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_11054_length_3768_cov_8.682736, whole genome shotgun sequence.
ACCESSION   DAOQEW010000302 DAOQEW010000000
VERSION     DAOQEW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11054_length_3768_cov_8.682736"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..2095
                     /gene="thrS"
                     /locus_tag="PLS35_18995"
     CDS             140..2095
                     /gene="thrS"
                     /locus_tag="PLS35_18995"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNI20816.1"
                     /translation="MASQLIHITLPDGTRKQVPTGCTVREALTPQGGRLDGKILAAKV
                     NGVPMDLFHALDQDASVEPLTFESAEGREVYRHSSTHIMAQAVKEVFPSAQLTIGPAL
                     DDGFYYDFAFDRPFTPEDLEKIEARAIEITKRGLTVSRSELSKEDAIKFFQDRGEAYK
                     VELINSFDDGSPISLYRQGEFVDLCRGPHLPTTGYVGAFKLLSTGGAYWRGDERNPML
                     QRVYGTSFPTKKELDAHLAKLEEIKRRDHRKLGKELDLITIQDDIGPGLVLWHPKGSL
                     IRLLIENFWREQHIKDGYDLVYSPHVARLDLWKTSGHVDYYRENMFASMKLEGSEYQL
                     KPMNCPFHIMIYKSHLRSYRDLPIRYGELGTVYRYERTGVLHGLLRVRGFTQDDAHLF
                     CRPDQIEAEVSRVLDFTFFVLGTFGFTEFEIYLSTRPEKSVGSDENWTIATNALEAAL
                     KSRNVAYQVDPGEGVFYGPKIDIKIKDVLGRSWQCSTVQVDFNNPERFKLAYTGEDGK
                     HHQPIMIHRALMGSIERFFGILIEHYAGAFPTWLAPVQAVVLTITDHQQEFAAKIVST
                     LKSHGYRVEADLRNEKIGFKIREAEKNKIPYMLVVGDKEVQSGMVAVRGRSKADHGTM
                     PIEQFLELIRTDTNQSLRDTATHSQTR"
     gene            2099..2605
                     /gene="infC"
                     /locus_tag="PLS35_19000"
     CDS             2099..2605
                     /gene="infC"
                     /locus_tag="PLS35_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNI20817.1"
                     /translation="MVPKLRVNREIRIREVRVIGPDGEQLGILPTVEAFNKAQEGGYD
                     LVEVAPTSQPPVCRIMDYGKYKFELSKKDHQSRRHQKSTQVKEIKLRPRTDKHDLEIK
                     IRQIKEFLADGNKTKVTLTYRGREMANQEMGRTMMTSVIQQCMEAGTVEFAPRMEGRS
                     LIMILAPK"
     gene            2639..2836
                     /gene="rpmI"
                     /locus_tag="PLS35_19005"
     CDS             2639..2836
                     /gene="rpmI"
                     /locus_tag="PLS35_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNI20818.1"
                     /translation="MKQKMKTHSGAKKRFRRTGTGKLVRRKAGGRHLLTGKPRDRKRN
                     LKGATEVSSASTPALNKLLPQ"
     gene            2880..3248
                     /gene="rplT"
                     /locus_tag="PLS35_19010"
     CDS             2880..3248
                     /gene="rplT"
                     /locus_tag="PLS35_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNI20819.1"
                     /translation="MPRTKGGPKTRQRRKKRLKLAKGQYGAKSRLFRTATESVDKGQA
                     YAYSGRKQRKRDFRQLWIARISAATRLHGIAYSRFMNALKKANILLDRKVLSDMAIRD
                     MAGFEKLVGVAKQQLATAAS"
     gene            complement(3304..>3768)
                     /locus_tag="PLS35_19015"
     CDS             complement(3304..>3768)
                     /locus_tag="PLS35_19015"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20820.1"
                     /translation="EWANVVEVDNVMSLSYSCLRQWITPSLLQVETNSSRAFYPHRMF
                     EVGEVAVPDARADVGSRTITMLGAVIAHAGAHFSEIHSCLDLLLYYLDRPFRLEPVPH
                     PSFLEGRAGRIMLDGRAIGLLGELHPEVLEQWQIGVPAVALELEIDRLLDEA"
BASE COUNT          929 a         1055 c         1054 g          730 t
ORIGIN      
        1 cgctaccttg acacggtaga ggtcgacggt tcaagaccgt ccgcgcctac cattttcgtt
       61 cgctcatgaa gttcgtgatc cgattacccg cagggggtgg tcggcgcgac gttgtatttt
      121 tttggcagga ttgaccggag tggcctcaca gctcattcac atcacccttc ctgacggaac
      181 tcgaaaacaa gtaccaacag gatgcaccgt ccgagaggcc ttaaccccgc agggaggccg
      241 tctcgacggg aaaatcttgg ccgcgaaggt caacggcgtg ccgatggact tgttccatgc
      301 gttggatcaa gacgcgtccg tcgaaccgtt gacctttgaa tccgcggaag ggcgtgaggt
      361 gtaccgccat agcagcaccc acattatggc tcaagcggtc aaggaagtgt tcccgagtgc
      421 gcaacttacg attggtccgg cgctcgacga cgggttctac tacgattttg cgttcgaccg
      481 gccgttcacc ccggaagatc tggaaaagat cgaagcccga gccattgaaa tcaccaagcg
      541 tggcctgact gtcagccgta gtgaactatc caaagaagac gcgatcaagt tttttcagga
      601 tcgcggggaa gcctataagg ttgagttaat caatagcttt gacgatggtt cccctatttc
      661 cctgtaccgt cagggagaat tcgtcgacct ttgtcgcggg ccgcacctgc ccaccaccgg
      721 ctacgtgggc gcctttaagc tcctctcgac cggcggcgcc tactggcgcg gggacgaacg
      781 caatcccatg ctccagcggg tgtacggcac ctcgtttccc acgaaaaagg aacttgatgc
      841 ccatctggca aaactcgaag agatcaagcg tcgcgaccat cgcaaactcg gcaaagagct
      901 cgacctgatc acgattcaag acgacatcgg tcccggcttg gtgctctggc atcccaaagg
      961 ctccctgatc cggctgctca tcgaaaattt ctggcgggag caacacatca aggacggcta
     1021 cgacctggtg tattcgccgc atgtcgcgag gctcgacctg tggaaaacga gcggccacgt
     1081 cgactattac cgcgaaaaca tgtttgcctc gatgaaactg gaaggcagcg agtatcagct
     1141 caagccgatg aactgcccct tccatatcat gatctacaaa tcgcacttgc gcagctatcg
     1201 cgatttgccg atccggtacg gagagttggg caccgtctat cgttacgagc gaaccggcgt
     1261 gctgcatggg ctgctacggg tccgcggctt cactcaagac gacgcgcatt tgttctgccg
     1321 acccgatcaa atcgaggccg aagtcagccg cgtgctggac ttcacctttt tcgtgcttgg
     1381 caccttcgga ttcacggaat ttgaaattta cctctcgacc aggcccgaga aatccgtggg
     1441 atcggatgag aactggacca tcgccaccaa cgccttggaa gcagcgctga agagccgcaa
     1501 cgtggcctat caggttgatc cgggtgaagg cgtattctac ggcccgaaaa tcgacatcaa
     1561 aattaaagat gtcctgggtc gatcatggca atgctcgacg gttcaggtcg atttcaacaa
     1621 tcctgaacgc ttcaagctgg cctataccgg cgaagacggt aaacaccatc aaccgatcat
     1681 gattcatcgg gcgctcatgg gatcgatcga gcgcttcttt ggcattttga tcgagcacta
     1741 tgccggcgcc ttcccgacgt ggctggctcc ggttcaagcc gttgtactga caatcaccga
     1801 ccatcaacag gaattcgcgg ctaagatcgt ctccaccctc aagagccacg gctaccgtgt
     1861 tgaagcggac ctccgcaacg agaagatcgg tttcaagatc cgcgaggcgg aaaagaacaa
     1921 gattccctat atgctggtgg tgggcgataa ggaagtgcag agcggcatgg tggcggttcg
     1981 cggtcgaagc aaagctgatc atgggaccat gccgatcgaa caattcctgg aactcatccg
     2041 cacagacacg aatcaatcat tacgcgatac agcaacccac tcacaaacga ggtgacctat
     2101 cgtccctaaa ttacgtgtaa accgggaaat caggattcgg gaagttcgtg tcatcggtcc
     2161 tgacggcgag caattgggaa tactgccgac cgtagaggca ttcaataagg ctcaggaagg
     2221 gggctacgat ctcgtcgagg tggccccgac ctcacagcct ccggtttgcc gtatcatgga
     2281 ctatgggaaa tataagttcg agctcagcaa gaaagatcac cagagccgtc ggcaccagaa
     2341 atccacgcag gtcaaagaga tcaagctgag accacgcacc gacaaacacg atctcgagat
     2401 caagatccgc cagatcaaag agtttttggc cgatgggaat aagaccaagg tgacgctcac
     2461 gtatcgtggt cgtgagatgg ccaatcagga aatgggtcgc acgatgatga cgagtgtgat
     2521 ccagcagtgc atggaagcgg ggaccgttga atttgcgccg aggatggaag ggcggagcct
     2581 gattatgatt ttggctccca aatagtgtgc gcgacgcacg caaggaagga atgagagcat
     2641 gaagcagaag atgaaaaccc acagcggcgc gaagaagcgg ttccgacgaa cgggaacggg
     2701 caaattggtt cgccgaaagg ccggcggtcg gcatttgttg accggcaagc cacgcgaccg
     2761 caagcggaac ttgaaggggg ccacggaagt ctcgtccgcc tcgacacccg cgttgaacaa
     2821 gttgcttccg cagtaacgat gattgtgccg tgaacaggat ctgaaaggag tagcgtccca
     2881 tgcctcgtac aaaaggtggt ccgaaaacac gacagcggcg aaagaagcgc ctgaaactgg
     2941 cgaaaggcca gtacggcgcc aaaagccggc tgttccgaac agcaaccgaa tcagtcgata
     3001 aagggcaggc ttacgcgtat tcagggcgaa agcagcggaa gcgtgatttc cgccagctct
     3061 ggatcgcgcg catcagcgcc gcgacccgct tgcatggcat tgcgtatagc cgcttcatga
     3121 acgccctgaa gaaggcgaat atcttattgg atcggaaagt gctgtccgat atggcgatcc
     3181 gggatatggc ggggtttgag aaactcgtcg gcgttgccaa gcagcagctc gccaccgcag
     3241 cgagctaacg ggttcgcctc aatgctctgt gacgggaaga tcctgatgga acggattttc
     3301 ccgtcacgct tcatccagta atcgatcgat ttccaactcc agcgcgactg ccggcactcc
     3361 aatctgccac tgctctaaca cctcgggatg caactctccg agcagtccga tagctcgtcc
     3421 atccagcatg attctgcctg ccctgccctc aagaaacgac gggtgaggga ccggttccaa
     3481 cctaaaagga cggtccaagt aatagagcag caagtccagg caggagtgaa tttcggaaaa
     3541 atgcgcgccg gcatgggcga tgaccgcccc caacatcgtg atcgtccgtg acccgacatc
     3601 ggcgcgagca tctgggacag ccacctcacc gacctcgaac atccgatggg gataaaacgc
     3661 ccggctcgaa ttggtctcca cctgcaacaa cgaaggggtg atccactggc gcaggcacga
     3721 atagctaagc gacatcacat tatccacttc cacgacgttc gcccactc
//
LOCUS       DAOQEW010000303         3726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_11298_length_3726_cov_9.910379, whole genome shotgun sequence.
ACCESSION   DAOQEW010000303 DAOQEW010000000
VERSION     DAOQEW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_3726_cov_9.910379"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..636
                     /gene="atpH"
                     /locus_tag="PLS35_19020"
     CDS             97..636
                     /gene="atpH"
                     /locus_tag="PLS35_19020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNI20821.1"
                     /translation="MIKTAVARRYAKALFELLDASSIEPARLALNGLGEAFTESSALR
                     HAVASPMFPEATKLSVLIEVATKLGCPPVVKRFLDQLVKKNRVSFLPDIADAFAKLVD
                     ESKGTQQVLVSSANALPATEQDRITTRLRDLLKRDVDVTFHTEPEHVAGLHIRLGSTV
                     VDSTVRGRLSAMQRVLTKE"
     gene            639..2156
                     /gene="atpA"
                     /locus_tag="PLS35_19025"
     CDS             639..2156
                     /gene="atpA"
                     /locus_tag="PLS35_19025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNI20822.1"
                     /translation="MQIKAEEISSIIKEKIKGFDQQVDVSQTGSVIQVGDGIAKVYGL
                     DGAMAGEMLEFPGGLYGIALNLEEDNVGAVLMGDDTGIKEGDPVKRTGRIAEIPVGEA
                     LVGRVVNAIGQPIDGKGPINSQHSSRIEVVAPGVNTRQSVREPLQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGLGVFCIYVAVGQKRSTVARVVKTLEENHAM
                     EYSMVVAATASDPAPMQFLAPFAGAAIGEYFRDNGKHALIVYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDKLGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLGSDLFYSGIRPAINVGLSVSRVGGSAQIKTMKQVAGTLRLDLAQYREM
                     AAFSQFGSELDKATQMQLARGVRMVELLKQGQYKPMPVADQVLSIYAGVNGFLDDVPV
                     DKVLQFEADLLHYVAQNHQDMRKDIATVGKIDDKVGGKLKEIITTFKQKMGYGGK"
     gene            2239..3123
                     /gene="atpG"
                     /locus_tag="PLS35_19030"
     CDS             2239..3123
                     /gene="atpG"
                     /locus_tag="PLS35_19030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNI20823.1"
                     /translation="MPSLQSLRRKIAAFKNTQKITKAMKMVAAAKLKRSQDRILAARP
                     YAHKMRGVLSNLSQRVNRTSHPLLQKREGKKIEILVVTSDRGLCGGFNGNIVRKSAEF
                     VRQCESQGLSVNLSLIGRKGRDYFRRRSLPIRQEWTGIFDKLSFEHAIDIGGDLTENF
                     VKGTFDELYVVYNEFKSAIQQRVIVEKLFPIDAATEFGASQATEGSSLGGSYLYEPDE
                     GELLSALVPKHFQIQTYRILLESAAAEHGARMAAMDGATRNAGQLIKKVTLYYNKTRQ
                     AAITKELMDIVGGAEALK"
     gene            3191..>3726
                     /locus_tag="PLS35_19035"
     CDS             3191..>3726
                     /locus_tag="PLS35_19035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNI20824.1"
                     /translation="MSTGKVIQVIGPVVDVEFPPGQLPNIYNALKVTQEENKAAGKPA
                     VRITLEVASHLGENRVRGIAMSTTDGLTRGMDVTDTGAPIAVPVGRETLGRLINVLGE
                     PVDEKGPIKTNKTYPIHRPAPRLEDQDTKTEVLETGIKVVDLLEPYSKGGKVGLFGGA
                     GVGKTVIIMELINNIALH"
BASE COUNT          868 a         1043 c         1067 g          748 t
ORIGIN      
        1 gcgtttgaat gctaacttgg cgcgcttttt gtcgtgccca ccgcacgcaa gcttcccgtt
       61 tgatcaaaac acggttaacc gtagcggata gttattgtga ttaagacagc cgtcgcacgt
      121 cgatacgcaa aagctctgtt cgaacttctt gatgcctcga gcattgagcc ggcccgcttg
      181 gccctcaatg gactaggaga agcctttacc gagtcatccg cattgcggca tgccgtggct
      241 tctccaatgt tccctgaagc cactaagtta agtgtgctga tcgaagtcgc cactaagctc
      301 ggatgcccgc cggtcgtcaa gcggttccta gatcaactcg tcaaaaagaa tcgtgtcagt
      361 ttcctgcccg acatcgccga tgcctttgcc aagctggtgg atgaatccaa aggcacgcag
      421 caagtgctcg tgtcatccgc gaatgcgttg ccggcgactg agcaggatcg cattaccacc
      481 cgattgcgcg atctgctgaa gcgcgatgtc gatgtcactt tccatacaga acctgaacat
      541 gtcgccggcc tccacatccg cctgggcagc accgtcgtag acagcacggt ccggggccga
      601 ctgagcgcca tgcagcgagt gttgaccaag gagtagccat gcagatcaag gcagaagaga
      661 tcagctccat tattaaagag aagatcaagg gcttcgatca acaggtcgat gtcagccaga
      721 ccgggtccgt gatccaggtc ggcgacggta tcgcgaaagt ctacggtctc gatggtgcga
      781 tggccggaga gatgctcgaa ttcccgggcg gcctctacgg gatcgcgttg aaccttgaag
      841 aagacaatgt cggcgccgtg ttgatgggcg atgacactgg tatcaaagaa ggcgatcccg
      901 tcaagcggac cggccgaatt gccgaaatcc cggtgggcga agccctagtc ggacgcgtgg
      961 tcaatgccat cggacagccg atcgacggca aggggcccat caattcgcaa cattcctccc
     1021 gtatcgaagt agtggcgcct ggcgtcaata ccagacagtc cgtgcgcgaa ccgttgcaga
     1081 cgggcatcaa ggccatcgat gccatgatcc ccatcggtcg tggtcagcgt gagttgatca
     1141 tcggagaccg tcagaccgga aaaactgcga tcgccgtcga caccatcatc aatcagaagg
     1201 gtctcggcgt gttctgcatt tatgtcgccg tcggacagaa acggtcgacc gtcgcgcgag
     1261 tggtgaagac gctcgaagaa aaccacgcca tggagtacag catggtggtg gcggcaacgg
     1321 ccagcgaccc ggcgccgatg cagttcctgg ccccgtttgc gggtgccgca atcggtgagt
     1381 atttccgtga caacgggaag cacgcgctga tcgtctacga cgatttgtcc aaacacgccg
     1441 tcgcctatcg acagctgtcg ctcttgctcc gtcgtccccc cggtcgtgag gcctatcccg
     1501 gcgatgtgtt ctatctgcac tcccgtctat tggagcgcgc cgcgaaattg agcgacaagt
     1561 tgggtggcgg cagtttgacc gcgctcccga tcatcgagac gcaggcaggc gacgtgtcgg
     1621 cctatattcc gacgaacgtc atctcgatca ccgacggcca gatttacctt ggtagcgacc
     1681 tgttctattc cggtattcgt ccagccatta acgtcggtct gtccgtgtcc cgcgtcggcg
     1741 gttccgcgca gatcaagacc atgaagcagg tcgccggtac gctccgactc gatctcgccc
     1801 agtatcgcga aatggcggcc ttttcccagt tcggcagtga actcgataaa gccacgcaaa
     1861 tgcagttggc ccgcggtgtc cgcatggtcg aactgctcaa gcaagggcaa tacaagccga
     1921 tgccggttgc cgaccaggtc ttgtcgattt atgcgggtgt caacggattc cttgacgatg
     1981 tgccggtgga caaggtgctc cagtttgagg ccgatctgct ccactatgtc gcgcagaatc
     2041 accaggatat gcgaaaagac attgccactg tcgggaagat cgacgacaag gtgggtggca
     2101 aactcaaaga aatcatcacg acctttaagc agaaaatggg ctatggagga aagtaagata
     2161 tcgtgattgc gtgatcgggg agtgaccagt cctctcttca ctgactcgtc caatcgcacc
     2221 tgtctgcttc cgtaaaatat gccgagctta caatcactca ggcggaaaat tgccgccttc
     2281 aaaaatacgc agaagatcac gaaggccatg aaaatggtcg cggcggcaaa gctcaagcgc
     2341 tcgcaggatc gtattctcgc tgcccggccg tacgcgcaca aaatgcgcgg cgtgctgagc
     2401 aatttgagcc agcgcgtcaa tcgtacctcc catcccttgc ttcaaaagcg tgagggaaag
     2461 aaaatcgaaa tcctcgtcgt cacgagcgat cgcggcctct gtggcggatt caacggcaac
     2521 atcgtccgaa agagcgccga attcgtgcgg cagtgtgaat ctcaaggact ctcggtcaat
     2581 ctcagcctga tcggacgcaa ggggcgagat tattttcgcc gtcgttcctt gccgattcgc
     2641 caagagtgga cgggcatttt cgataagctg agttttgagc acgccatcga tattggcggc
     2701 gatctcaccg aaaatttcgt caaagggaca ttcgacgaac tctacgtggt gtacaacgag
     2761 ttcaagtctg cgattcaaca gcgcgtgatt gtcgaaaaac tgttccctat cgacgccgct
     2821 acggagttcg gtgcgtcgca agccaccgag ggttcgtccc ttggaggcag ctatctgtac
     2881 gagcctgatg agggcgagct tctgagcgca ttggtgccca agcactttca aattcagacg
     2941 taccgcattt tattggaatc ggcggctgca gaacatggcg cacgtatggc ggccatggac
     3001 ggcgccaccc gtaatgccgg acaactgatc aagaaagtga ccttgtatta caacaagact
     3061 cgccaagcgg ccatcaccaa agaactcatg gacatcgtgg gtggagcaga ggccctgaaa
     3121 taaatctgga ctgaggaatg ctgcacgacg gtaagctgcc gggtgcatca gtgacgacaa
     3181 gaggagcgtt atgagcacag gaaaagtcat tcaagtcatc ggccccgtgg tcgacgtgga
     3241 gtttcctccg ggtcaactgc caaatatcta caacgcgctg aaggtgacgc aagaagagaa
     3301 taaggcggcc ggaaagcctg ccgtgcggat cactctggaa gtcgcctctc acctcggaga
     3361 aaatcgtgtg cgcggcatcg cgatgtcgac caccgatggt ctcacgcgcg ggatggacgt
     3421 gaccgacacc ggtgccccga tcgcagttcc cgtaggccgc gaaacgttgg gtcggctgat
     3481 caacgtgctc ggcgaaccgg tagacgaaaa gggtccgatc aagaccaaca agacgtaccc
     3541 gatccatcgt cccgctccga gattggaaga tcaggacacg aaaaccgaag tgctggagac
     3601 cggcatcaag gtggtcgact tgttagagcc ctacagcaag ggcggaaaag tcggcctctt
     3661 cggcggtgcc ggagtcggca agaccgtcat catcatggag ctcatcaaca acatcgcgct
     3721 ccacca
//
LOCUS       DAOQEW010000304         3720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_11329_length_3720_cov_9.231651, whole genome shotgun sequence.
ACCESSION   DAOQEW010000304 DAOQEW010000000
VERSION     DAOQEW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11329_length_3720_cov_9.231651"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..80)
                     /locus_tag="PLS35_19040"
     tRNA            complement(4..80)
                     /locus_tag="PLS35_19040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Pro,seq:cgg)
     gene            complement(116..1219)
                     /gene="ald"
                     /locus_tag="PLS35_19045"
     CDS             complement(116..1219)
                     /gene="ald"
                     /locus_tag="PLS35_19045"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNI20825.1"
                     /translation="MLIGVPKEVKDFEYRVSVTPDGVRLLRQHGHQVLVEPSAGQGSG
                     FSDEAYRQAGADVVGSKEEVFKRAELIVKVKEPQLAECALFRPGQVLFTYLHLASLPD
                     LTKALMAAEITAIAYETVEAKDHSLPMLRPMSEIAGRLAVQVGAHYLGTVQGGRGVLL
                     AGVPGVLPGHVVVLGAGVVGTSAVRIAVGMGARVTVINLDLDRLRLLDDLYGGRIATC
                     AATESAIERAVMQADLVIGAVLVPGARAPRVISRSLVSKMQAGAVIVDVAVDQGGCCE
                     TTRPTTHSDPVYTVDGVVHYCVTNMPGIVPHTSTRALTNVTLPYLVRLASEGVGKAIH
                     SDPGLAKGVNVMNGKITCQAVAESHGLRFTPLL"
     gene            1387..1755
                     /locus_tag="PLS35_19050"
     CDS             1387..1755
                     /locus_tag="PLS35_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20826.1"
                     /translation="MDAGSCNACNTAGTPLMKLSLGKDFFGRTYDRLSPSSDQSPKWY
                     CEPCSMMKNLQRDFRDIRTEFDKLATGQTSALSEPEAKQRAQLRLREISAIAGSQTAG
                     SSLLNPADVKQLIDQFHARA"
     gene            1803..2153
                     /locus_tag="PLS35_19055"
     CDS             1803..2153
                     /locus_tag="PLS35_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNI20827.1"
                     /translation="MTGYQRHIFVCTNKREPDDPRGSCSKLGSESLHACFKQEAKRLN
                     LKGIVRANKAGCLDYCAQGPSVVVYPEGIWYRVRTEADVKEIMERHIVNGEPVERLLM
                     PDQARPSLLSPLKP"
     gene            2217..2600
                     /locus_tag="PLS35_19060"
     CDS             2217..2600
                     /locus_tag="PLS35_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20828.1"
                     /translation="MEKVAFMKAFPGLLRTWDQLKGKTVEAVLPLKSKTGSSAIICTD
                     GSFVVVPLLSPEPYELGEALQAGREYLEPKHPEAYVGYDRLRKKDQDAQRTARLENIL
                     GAIQNNVEQIPELKDRLAQLVKEWK"
     gene            2597..2893
                     /locus_tag="PLS35_19065"
     CDS             2597..2893
                     /locus_tag="PLS35_19065"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20829.1"
                     /translation="MSSLPQKNMFEIFSQGLFEGVKPMLVIRDHLVRHPDRCTHQAIC
                     VPICPTSAWLSTPPYKFDPSRCLESCRLCLDACPSQAIYAVFRKGEKLLEPQKK"
     gene            complement(2918..>3720)
                     /locus_tag="PLS35_19070"
     CDS             complement(2918..>3720)
                     /locus_tag="PLS35_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNI20830.1"
                     /translation="AVQAIQTGLLPHQTDSAERVLLERLPECLALYRQFEQGDRDRIR
                     WLMEVLPNGMEMDLARFPGSSAQDLTALERPEELDRYTYFVAGCVGEFWTRMVCAHRP
                     AMRRWDVDRMSAIGVRFGKGLQLTNIVKDVARDLHNGRCYVPTQWLDEVGLKPTDLLK
                     PESLPTFRPILLRMIRQAVEHLDQGWLYTMALPRLEIRQRLACMWPILLAGETLKRVA
                     TAPDLLDPSVNVKAPRSVVYRVMALTTVTGGCGYVGTAYWGRLRKQIM"
BASE COUNT          820 a         1176 c         1016 g          708 t
ORIGIN      
        1 gtttggtcgg ggcgagagga tttgaacctc cgacccctgc gtcccgaacg cagtgcgcta
       61 ccgggctgcg ctacgccccg accgggcgga accgaaaaac tgaagccgga ttgtcttaca
      121 acagaggggt aaaacgcaag ccgtgagatt cagcgaccgc ctggcacgta atcttcccat
      181 tcatgacgtt cacgcctttg gccaggccgg gatcggagtg aatggctttc ccaacccctt
      241 ccgatgcgag acggacaaga tagggaaggg tcacgttggt caacgctcgc gtggaagtgt
      301 gcgggacaat ccccggcatg ttggtcacgc aatagtgcac gacaccatcc accgtataca
      361 caggatctga atgcgtcgtg ggcctggtgg tttcgcagca cccgccctgg tccacggcga
      421 cgtccacaat gacggctcca gcctgcatct tcgacacgag actacgcgaa atcacgcggg
      481 gtgcacgcgc gcccggcacc aacacggctc caatcaccaa gtcagcctgc atgaccgctc
      541 gctcaatggc cgattcagtg gcggcacagg tcgcaatgcg tccgccgtac aggtcatcga
      601 gcaggcgcaa tcggtccagg tccagattaa tgaccgtgac cctggcgccc attcccacgg
      661 cgattctgac cgccgacgtg cctacgactc ccgcgcccaa taccacgaca tggcccggca
      721 gcacaccagg cactccggct aacaggacac cgcgtccgcc ttgaaccgtc ccgagataat
      781 gcgcgccgac ctgcacggca agacggccgg caatctcgct catgggccgc agcatcggca
      841 ggctatgatc ctttgcttcg acggtttcat aggcaatagc cgtaatctct gccgccatca
      901 acgccttggt caggtccggc aaggaagcga ggtgcaaata agtaaacagg acctgaccgg
      961 gccggaacaa cgcacattcg gctagctgcg gctccttcac tttcacgatg agttcggccc
     1021 gtttgaacac ctcttccttg gagcccacga cgtcagcgcc ggcttgacga tacgcctcat
     1081 ccgaaaaccc gctcccttgt ccggccgatg gctcgaccaa cacctgatgg ccatgctgtc
     1141 gcaacagacg cacgccgtca ggcgttacac tcacgcggta ttcgaaatct ttaacttctt
     1201 taggaacgcc aatcagcatg cgcgcctcct cgccccagtc accagtgctc ctgttgttgt
     1261 ctacattata ccccgaaccg cactctgtat atgcgtgcct cgctccacca ccttgcgagg
     1321 ggaaagagca gtgcggtaag attcgatctc tttggttctt tccccttgaa gaggagcact
     1381 tcgcgcatgg atgccggatc ctgtaatgcc tgcaacaccg caggtacacc gttgatgaaa
     1441 ttgtcgttgg ggaaagattt tttcggtcgc acctatgacc gattgtcgcc ctcctccgac
     1501 cagagcccga aatggtattg tgaaccctgc tccatgatga agaatctgca gcgcgatttt
     1561 cgtgatatcc ggacggaatt cgacaaactc gccacgggac agacctcggc cctatcggag
     1621 ccagaggcga aacagcgcgc gcaattgcgt cttcgggaaa tttccgccat tgccggcagt
     1681 caaactgccg gctcgtcact cctgaaccca gcggacgtga agcaactgat cgaccagttc
     1741 cacgcgcgtg cctgagctcg caattaccag cttgaaccac ataacacact gacatcctga
     1801 cgatgaccgg ctatcaacga catatatttg tatgcaccaa caagcgcgag cctgatgatc
     1861 cccgcggcag ctgttccaag cttggatcag agtcgcttca tgcctgtttc aaacaagagg
     1921 ccaagcgact caatttgaag ggcatcgtgc gtgcgaataa ggccggttgc cttgactatt
     1981 gcgcccaagg acccagcgtg gtggtctatc ccgaaggcat ctggtaccgc gtgcggactg
     2041 aagccgacgt gaaggaaatc atggagcgcc atatcgtgaa cggagaaccg gtcgaacgac
     2101 tcctgatgcc tgatcaggcc cggccttccc tcctctctcc gctgaaacct tgagccgggt
     2161 cgtccaccat agctcgcgag atctgtatgc cagccgatga aaaatggaag gccaacatgg
     2221 aaaaggtggc cttcatgaaa gcgtttcccg gattgctccg cacctgggat cagctgaagg
     2281 gcaaaaccgt ggaggccgtg ttaccgctca agagcaagac cggatcctcc gcgatcatct
     2341 gcaccgatgg atcgttcgtc gtggtcccgc tgctgagtcc ggaaccatac gagttaggcg
     2401 aagcgcttca ggcaggccga gagtacctcg agccgaagca ccctgaggcc tacgtcggat
     2461 atgaccggtt gcgcaagaag gaccaagatg cgcaacgcac cgcgcgcctc gaaaatattc
     2521 tcggcgccat tcaaaataat gtggagcaga ttccggaatt gaaggaccgg ctggcacagc
     2581 tggtcaagga gtggaagtga gcagtctccc gcaaaaaaac atgttcgaga tcttttcgca
     2641 gggtctcttc gaaggagtga aaccgatgct ggttatccgc gatcatcttg tccgccatcc
     2701 tgaccgctgc acccaccaag ccatttgcgt gcccatttgc ccgaccagcg cctggctttc
     2761 gacccctccc tacaaattcg acccgtcccg ctgcctggaa agctgccggc tctgcctgga
     2821 tgcctgcccc tcccaagcga tctatgccgt gttcagaaaa ggtgagaaac tgttagagcc
     2881 gcagaagaag taacgggtcg atcgacgccc tacgcgtcta catgatctgc tttcgtaaac
     2941 gcccccagta ggccgtcccc acatagccgc aaccgccggt cacggtcgtg agcgccatca
     3001 ctcggtagac caccgagcgc ggggctttga cattcacgct ggggtcgagc agatcgggcg
     3061 cagtggccac acgtttcagc gtttcgccgg ccaacagaat cggccacatg caggcaaggc
     3121 gttgccggat ttccaaccgc ggcagcgcca tggtgtagag ccaaccctga tccaaatgtt
     3181 ccaccgcctg acggatcatc cgcaggagga tggggcgaaa ggtcggaagg ctttcgggtt
     3241 tgagcagatc cgtcggtttg agccccactt catccagcca ctgcgtcggc acgtaacacc
     3301 gaccgttgtg aaggtcgcgg gcgacgtcct tcacaatgtt cgtaagctgc aatcccttac
     3361 cgaagcggac gccgatcgcg gacatccggt cgacatccca gcgcctcatt gccggccgat
     3421 gtgcgcagac catgcgggtc caaaactctc ccacacaacc ggcgacgaaa tacgtatacc
     3481 gatccaattc ttcgggacgc tccagcgcgg tgaggtcctg ggctgatgaa ccgggaaacc
     3541 tggccaggtc catttccatg ccgttcggca gcacctccat gagccagcga atgcgatcgc
     3601 gatccccctg ctcaaactgc ctgtaaagag ccaagcactc cggcagccgc tccagaagca
     3661 cgcgctcagc cgagtcggtc tggtgcggca gcagtccggt ctgaatggcc tgcacggcct
//
LOCUS       DAOQEW010000305         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_11804_length_3641_cov_5.921361, whole genome shotgun sequence.
ACCESSION   DAOQEW010000305 DAOQEW010000000
VERSION     DAOQEW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11804_length_3641_cov_5.921361"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLS35_19075"
     CDS             complement(<1..215)
                     /locus_tag="PLS35_19075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNI20831.1"
                     /translation="MAKLILSMDGLVLKEIPLNKERMTIGRKPHNDIQIDNLAISGEH
                     SVIVTILNDSFLEDLNSTNGTYVNGQPV"
     gene            complement(228..1046)
                     /locus_tag="PLS35_19080"
     CDS             complement(228..1046)
                     /locus_tag="PLS35_19080"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNI20832.1"
                     /translation="MDLSQYLEIATRTDPGMVRSHNEDSVMANAAIGLAILADGMGGY
                     NAGEVASGMATTLLGTELESALSLRAPHGFEGGKRVAHHVLEAEIAKANTSIYQAAQS
                     QPQYSGMGTTLVVALFYDNLMTVAHIGDSRLYRLRGEEFKQVTRDHSLLQEQIDSGMI
                     TPEQAKHSLNKNLVTRALGVDPVVEPEIKDYDTLPGDIYLLCSDGLSDMVEDEEIGMT
                     LSALSANLELCATQLVQMANDNGGRDNVSVILVRVLKEYPVARGWWAKLLAWFK"
     gene            complement(1054..>3641)
                     /locus_tag="PLS35_19085"
     CDS             complement(1054..>3641)
                     /locus_tag="PLS35_19085"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNI20833.1"
                     /translation="VRGMNKATFWKNDWFLGVVVSVVLLFSGGSDLIQSLERKAYDLG
                     VGMSSRNPSDRVAVIAIDKASLDNIGRWPWSREILADTVDKLAAAKAKVIATTILFAE
                     PQLDAGLVYVNKLIDTYAQAGGTALTGTMEEIAAQPAPLSSFGPLLKEAEEKLNTDRR
                     FADACAKAGNMAFPMLFKIGEPRGRPDKELPDFVKKNAVKLAGGEALPLPTSDVDSIV
                     IEPLGRCAAVIGHLNSTPDVDGGIRTEPLVLNHFDQTYPALSLMVAAKSLNLGPADIQ
                     VRAGESVSLGRLKIGTDPVTRMYPFFYKDKDGHPAFQVDSFFDVKSGKIPYEKYKDKI
                     VLIGPTAAGVGSVFVTPVSATMPAVLLQAHIVSSILSEHFFVAPTWGFWVETLIFLLV
                     AAYLIALLPRLKAGPAAGATLGLLVLLLAAHFVLMTTQLMWLQLMLPAVLLVVGHLLL
                     TTKRFLVTERGKEKSDLDSAESNRMLGLAFQGQGQLDMAFDKFRKCPLDDQLMENLYN
                     LGLDFERKRQFNKAESVFRYMADYNPKFRDLENRLSRAKQMSETVILGGGGGGRTNAG
                     TMILEGGQVEKPMLGRYQVEKELGKGAMGVVYLGRDPKINRVVAIKTMALSQEFDEDE
                     LADVKERFFREAETAGRLNHPFIVTIFDAGEEHDLAYIAMEFLKGKDLVPYTKAGNLM
                     PLPKVMSIVARVAEALSYAHENNVVHRDIKPANIMYEPESDQVKVTDFGIARITDSSK
                     TKTGMVLGTPSYMSPEQLAGKKIDGRSDLFSLGVTLYQMASGKLPFEGDSMAQLMFKI
                     ANEPHPDIRTVNPDLPECLVAIVDKALTKDSEARYRTGADMARDLKDCLGGGAAGTTQ
                     VDIDV"
BASE COUNT          628 a         1178 c         1120 g          715 t
ORIGIN      
        1 accggctgcc cgttcacgta ggtgccgttg gtgctgttga ggtcctccag gaacgaatcg
       61 ttcaggatgg tcacgatgac cgagtgctcg ccgctgatcg ccagattgtc gatctggatg
      121 tcgttgtgcg gcttgcggcc aatcgtcatc cgttccttgt tgagcggaat ctccttgagc
      181 accaatccgt ccatgctgag aatgagcttc gccatcgtgg tcttccttca cttaaaccag
      241 gccaggagct tggcccacca gccgcgcgcc accgggtatt ccttcagcac gcgaaccaga
      301 atcacggaaa cattatcgcg gccaccgttg tcgttggcca tctgcaccaa ttgcgtcgcg
      361 cacaattcca gatttgccga cagcgccgac agggtcatcc cgatttcctc atcctcgacc
      421 atgtcgctca atccgtcgga gcacaagagg tagatgtcgc cgggcaaggt gtcgtagtcc
      481 ttgatttccg gctcgaccac cggatccacc cccagggccc tcgtcaccag attcttgttg
      541 agcgagtgct tggcctgttc cggcgtgatc atgccgctgt ctatctgttc ctgcaacagg
      601 gaatggtccc gcgtcacctg cttgaactcc tccccacgca ggcgatagag gcgcgaatcg
      661 ccgatatggg ccacggtcat caggttgtcg tagaacaggg ccacgaccag cgtggtgccc
      721 atcccggagt attggggctg gctctgggcg gcctggtaga tcgaagtatt ggccttggcg
      781 atctcggcct ccagcacgtg gtgcgcgacc cgcttcccac cctcgaatcc gtgcggcgcc
      841 ctgaggctca gggccgactc gagttcggtg ccgagcaagg tcgtcgccat gccgctggcc
      901 acttcgccgg cgttataacc gcccatgccg tcggcgagga tcgcgagacc gatggcggcg
      961 ttcgccatca ccgagtcctc attgtgggaa cgcaccatcc ccgggtcggt ccgggtggcg
     1021 atctccaggt attgactgag atccatggcc ccctcagacg tcgatgtcga cctgagtcgt
     1081 gccggcggcg ccgccgccca ggcagtcctt caggtcgcgg gccatgtccg cgccggtcct
     1141 gtaccgggcc tccgagtcct tggtcagggc cttgtcgacg atggccacga ggcattccgg
     1201 caggtcggga ttcacggtcc gaatatccgg gtgcggctcg ttcgcgatct tgaacatcag
     1261 ttgcgccatg gaatcgcctt cgaaaggcag cttgccgctg gccatctggt acaaagtcac
     1321 ccccagcgag aacaggtcgg aacggccatc gatcttcttg ccggccaatt gctccggtga
     1381 catgtaagac ggggtgccca ggaccatgcc cgtcttcgtt ttcgacgagt cggtgatgcg
     1441 cgcgatcccg aagtccgtga ccttcacctg gtccgactcc ggctcgtaca tgatgttggc
     1501 cggcttgatg tcccgatgca cgacgttgtt ctcgtgggcg taggaaagcg cctcggcgac
     1561 ccgggcaacg atgctcatca ccttgggcag gggcatcagg ttaccggcct tggtataagg
     1621 caccaaatcc ttgcccttca ggaactccat ggcgatatag gccaggtcgt gctcctcgcc
     1681 cgcgtcgaag atggtgacga tgaaggggtg gttgaggcgc cccgccgtct cggcctcccg
     1741 gaagaagcgc tccttcacgt cggccagttc gtcctcgtcg aactcctgcg acagcgccat
     1801 ggttttgatg gccaccaccc ggttgatctt cgggtcccgg cccagataga cgacccccat
     1861 ggcacccttg ccgagttcct tctcgacctg gtagcgcccg agcatgggtt tttccacctg
     1921 cccaccttcc aggatcatcg tgccggcgtt ggtgcgtccg cccccgccac cgcccaggat
     1981 caccgtctcc gacatctgct tggcccgcga cagccggttt tccaggtcac ggaacttcgg
     2041 gttgtagtcg gccatgtaac ggaacaccga ctccgccttg ttgaactgcc gtttgcgttc
     2101 gaagtccaga cccaggttgt agaggttctc catcaactga tcgtcgaggg gacacttgcg
     2161 gaacttgtcg aaggccatgt ccaactggcc ctgaccctgg aaggcgagac ccagcatccg
     2221 gttggactcg gcggagtcca ggtccgactt ctccttgccg cgctcggtga ccaggaaacg
     2281 cttcgtggtc agcaacaggt ggcccaccac cagcagcacg gccggcagca tcagttgcag
     2341 ccacatcagt tgggtggtca tcagcacgaa gtgtgccgcc agcagcagca cgagcaggcc
     2401 cagcgtggct ccggcggccg gtcccgcctt gaggcgcggc agcagggcga tcaggtaggc
     2461 tgccaccagc aggaagatca gggtctcgac ccagaacccc caggtgggtg ccacgaagaa
     2521 atgctcggac aggatgctgg agacgatgtg ggcctgcagc agcaccgccg gcatggtcgc
     2581 agacaccggc gtcacgaaca cacttccgac ccccgccgcg gtcgggccga tcaggacgat
     2641 cttgtccttg tacttctcgt agggaatctt gccgctcttc acatcgaaga aggaatccac
     2701 ctggaaggcc gggtgtccat ccttgtcctt gtagaagaac gggtacatgc gggtgaccgg
     2761 gtccgtgccg atcttcagcc gccccaggct caccgactcc ccggcgcgca cctggatgtc
     2821 ggccgggcca aggttcaagc tcttggccgc caccatgagc gacagcgccg gataggtctg
     2881 gtcgaaatgg ttgagcacga gcggctccgt gcggataccg ccatccacgt cgggcgtcga
     2941 attcaggtgg ccgatgaccg ccgcgcaacg accaaggggc tcgatgacga tggagtccac
     3001 gtcggacgtg ggcagtggca gggcctcgcc gccggcgagc ttcaccgcgt tcttcttcac
     3061 gaagtccggc agttccttgt ccggccggcc gcgcggctcg ccgatcttga acaacatcgg
     3121 gaaggccatg tttccggcct tggcgcaggc atcggcgaag cggcgatccg tgttcagctt
     3181 ttcctccgcc tccttgagca gggggccgaa ggaagataac ggcgccggct gggcggcaat
     3241 ctcctccatg gtgccggtga gggcagtgcc gccggcctgg gcgtaggtgt cgatcagctt
     3301 gttgacgtag acgaggcccg cgtccagttg cggctcggcg aacaggatgg tggtggcgat
     3361 caccttggcc ttggccgccg cgagcttgtc gacggtgtcg gcgaggattt cccgggacca
     3421 aggccagcgg cctatgttgt ccaagctggc cttgtcgatg gcgatcacgg ccacccggtc
     3481 cgacgggttc cgggaggaca tcccgacccc caggtcatag gccttgcgtt ccagactctg
     3541 gatcaggtcg ctaccgcccg aaaagagcag gaccacgctg acgacaacgc cgaggaacca
     3601 gtcgtttttc cagaatgtcg ccttgttcat cccccgaacc c
//
LOCUS       DAOQEW010000306         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12026_length_3601_cov_8.545967, whole genome shotgun sequence.
ACCESSION   DAOQEW010000306 DAOQEW010000000
VERSION     DAOQEW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12026_length_3601_cov_8.545967"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..988
                     /locus_tag="PLS35_19090"
     CDS             347..988
                     /locus_tag="PLS35_19090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNI20834.1"
                     /translation="MQRAVIADDHHLYRRGTRDVIQEVFAPEVIAEANTGTQLLNLVK
                     ACNWDLCIMDVSMPVKSGPDLVEEVVQIRPSMPILVLSMHPEEHYAVRMIRSGARGYL
                     NKSTTDSALLTEAIRSLLAGHKFITSTVAECLADTVCAGSHPSSALHQVLSNRELQIF
                     QMLAAGTPIKEIASTLFISAATVSTYRTRVLTKLALDSNADLVRYALEHSLIA"
     gene            complement(1450..2049)
                     /locus_tag="PLS35_19095"
     CDS             complement(1450..2049)
                     /locus_tag="PLS35_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HNI20835.1"
                     /translation="MLEKLKFFGHAPKCEAPIRATLLYHYQGAVPQGPNHIVPPPVEI
                     LDAPSRVQTAQATRIHLPGILVFIFVVGMLVLGYWWGQTVKPVGIIRAHLQAIEDGEY
                     GKAYSYLSLRAQEQVSYDEFVTLIQENSVVMEPRGSTFLSRKVNGNTASINGFVEGYS
                     EYVSDVRYLFVKEAHQWKIVRFEWGVPRPMKEDQSNASG"
     gene            complement(2299..2619)
                     /locus_tag="PLS35_19100"
     CDS             complement(2299..2619)
                     /locus_tag="PLS35_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20836.1"
                     /translation="MSGRTITLRRVIWSLLAIWLFFGGVSFTEQVNSLLDTSDQDEQA
                     LSQLSLTLKPEIPPLPKQVTSSVVPIVLIPSVIHLADSSPSLSIPSQLLTLRPHQRVS
                     VYRI"
     gene            complement(2745..3191)
                     /locus_tag="PLS35_19105"
     CDS             complement(2745..3191)
                     /locus_tag="PLS35_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20837.1"
                     /translation="MSRAVSLGDPSAVRRPEKQELEVITMAWYHERIAEEKLTVLIRY
                     LVHSFPLASILSRQVGDRYHVFIIVPFDGSPEKTLQVETTVFRNRSLRVEGFTALLST
                     LNLRRLFDQNDRYDLNWFGRQPKARHAATFYGASLPQASQISAALA"
BASE COUNT          859 a          964 c          998 g          780 t
ORIGIN      
        1 ggactttgtc cgccacggcc acggccgcga cggtgcccgc cgagattttg agaaattgtc
       61 gccgtgaaaa taacatagat gactccttat gtaaggggtg gaggattggg ggataggacg
      121 cacggtgcag gggcctcctt tctcttgaag caaaaaaaag gcaaagggat aatagcgggc
      181 ctctgcgaga aacaacgtcc ctattcaggg aaaatcctgt cattttttaa tgacagcgcg
      241 tctacatgaa cgggcgtgtc actaagggat atccctagtt gattccaaag aaggacagtg
      301 gtgaagtagc aggcggcgaa cttctagaga aaggagttca agacacgtgc aacgggccgt
      361 cattgcagac gatcatcatt tgtaccgtcg tggcacccgg gacgtgatcc aggaggtgtt
      421 tgcgccagaa gtcatcgctg aggcgaatac agggacacaa ttactcaatc tcgtgaaagc
      481 ctgcaattgg gatctgtgca tcatggacgt gtcgatgccg gtaaagtccg gccccgattt
      541 agtagaagaa gtggtacaaa tccgtccgtc catgccgatt ctggtcttgt cgatgcaccc
      601 cgaagagcat tatgcagtgc ggatgatccg atccggcgcg cgggggtatc taaacaaatc
      661 caccacggat tccgccttac tgaccgaagc cattcgctca ttgctcgccg gacacaaatt
      721 tatcacgtcc accgtggcag aatgtttagc cgacaccgtc tgtgccggat cacacccatc
      781 ttcagccctt catcaggttc tctcgaacag agagctccaa atcttccaaa tgctggccgc
      841 gggaacaccg atcaaggaaa ttgccagcac cctgtttatt agcgctgcca cggtcagcac
      901 gtatagaacc cgcgtattga ccaagttggc tctcgacagc aatgcggatc tcgtgcgtta
      961 tgcgctcgag cattcgttga ttgcctgacc tgtcatgcag tgaagtcacg ggcagctcct
     1021 gacagtgaat ccgtcaggat gcttgcagaa gtgattacct tccgttccct ctgccctact
     1081 gattcccaac tcccacggtc tcgggttgca gccagccccg tacgagctcc ggttcatctg
     1141 ccgcagtgag tcggatacgc ttttgaccgc accaggcaca ggtccagcgc tcgaactcgc
     1201 attggccttg gctgttatcg gaaacccaat ggtgtgtatg aggagttctc ttcgagggct
     1261 tggtcccgac aggaaacgag taggtggcaa caaatttttc gttcagcatg aggccgagga
     1321 taccgaagtt ctgcggcaag gtgggttagg aagacccctg gttgtaaaga ttgtaacccc
     1381 tcattgcact accggaggct caaggtcgag gggctcccac ctcgcttgag atcatttgat
     1441 accccagtct catcctgaag cgttgctctg gtcttctttc atgggtcgtg gcactcccca
     1501 ctcaaaacgc acgatcttcc attgatgggc ctccttgacg aacaggtatc ggacatcact
     1561 cacatactca ctgtaccctt ccacaaagcc attgatggag gcggtgttac cgttcacttt
     1621 tctggagagg aaggtgctgc cgcgcggttc catcacaaca ctgttctcct ggatcaatgt
     1681 cacaaattca tcgtatgaca cctgttcttg cgcccgtagt gatagatagc tatacgcctt
     1741 tccatactca ccatcttcga tcgcctgaag gtgggctcga atgatgccaa ccggtttcac
     1801 ggtttgtccc caccaatacc ccaggaccag catccccacc acgaatatga aaaccaagat
     1861 tcccggcagg tgaatgcgtg tggcttgggc ggtttgcacc cggctagggg catcgaggat
     1921 ttcaaccggt ggtggaacaa tatggttggg tccctgaggg acggcgccct ggtaatgata
     1981 gagaagagtc gcccgtatcg gggcttcaca tttaggcgcg tggccgaaaa acttgagctt
     2041 ctcaagcata gcgtctccat agagtcggaa gagctcaggc atggtggtca gtccgggtaa
     2101 tctgttcaac agcctcgaac actcttgaac ccggtgaggg cgacagtcag acgaacaacg
     2161 cgagcgtaga aagacgactc gtatcgatcg ccgacgcaat tccgaatcac ctggccaatc
     2221 accatgcgca cctatccacg caagtcagcg gcacaaagcc gctacgcgcg ggatcacgag
     2281 actaggatgg caaggagatc aaatgcggta gacggagaca cgttggtgag gacgaagcgt
     2341 cagaagctgc gatggaatcg agagggacgg tgaactatcg gcgagatgga tgactgaggg
     2401 gatcagcacg atggggacca cggaactcgt cacctgcttt ggcagcggag gaatttccgg
     2461 cttgagggtc aggctcagct gggaaagcgc ctgctcatcc tgatcgctcg tatccaacag
     2521 gctgttcact tgttcagtga atgagacccc accgaaaaac agccatattg cgagcaggct
     2581 ccagatgacg cgccggaggg tgatggttcg accactcatc gttgaacagt aatacgaacc
     2641 acacaaggag tcaatgatgc cactgtcgcg acaccttgct cccttctcat ggattcatcg
     2701 ggctctgggg aaaaaccaac ggcttcggtg ccccgcctgc tgtctcacgc gagcgctgcc
     2761 gaaatctgag aagcttgcgg tagggatgcc ccgtagaatg tggccgcatg cctcgccttt
     2821 ggctggcgac cgaaccaatt gagatcgtag cgatcattct gatcgaacag gcgacgaaga
     2881 ttcagtgttg acagaagagc ggtaaatcct tcgacccgaa gacttctgtt gcgaaagact
     2941 gtcgtctcga cctgcaatgt tttttctggt gacccatcga atggcacgat aataaacacg
     3001 tgataccggt ctccgacttg ccgagacaaa atactggcca gggggaaact gtgaaccaga
     3061 taccggatca acaccgtgag tttctcttcg gcgattcgtt cgtggtacca agccatggtg
     3121 atcacctcca gttcctgctt ctcggggcgc cgcactgccg atggatcgcc aagcgaaact
     3181 gccctgctca tcgttcagac caacgggctg gcctggtcag caacccaagc gtggcgagac
     3241 cacagatcaa tgctaagagc agtagactcg ccaccagaaa cagccatgct tttatctctt
     3301 gcgtcatgcc cgtatcctac ggcttcccgg ttcggccatg gacctaggga aaccgctgga
     3361 atgtgattag gagtactctc gtttacaggc tctcgtagag ctgccgatgc gccttacgac
     3421 gtgaagttgc cgaggaatgt gcgcccagat agcgagctta tgcagaagtg tagaaagaga
     3481 ggcggttaac agattattgg ggatggacga accggacgga atggcttgcg tggatgagaa
     3541 ggaaagtggt ggagggcagg ggagttgaac ccccgacccc tacgttgcga acgtagtgct
     3601 c
//
LOCUS       DAOQEW010000307         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12343_length_3545_cov_8.239542, whole genome shotgun sequence.
ACCESSION   DAOQEW010000307 DAOQEW010000000
VERSION     DAOQEW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12343_length_3545_cov_8.239542"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..3367
                     /gene="carB"
                     /locus_tag="PLS35_19110"
     CDS             98..3367
                     /gene="carB"
                     /locus_tag="PLS35_19110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNI20838.1"
                     /translation="MPRRTDIRSILLIGSGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILINSNPATIMTDPDFADRTYIEPITLDVVEKVIEYERPDALLPTMGGQTALNTAIGL
                     AKRGVLEKYNVKLIGASIEAIHKAEDRDAFRQAMWKIGLRVPDSGVATSLQEAEEVVR
                     RTQFPAIVRPSFTMGGTGGNIAYNIEEFRTQVEWGLAMSPVRQVLVEQSVIGWKEFEL
                     EVMRDLKDNVVIICPIENLDPMGVHTGDSITVAPALTLTDKEYQMLRDAAVRIIREIG
                     VDTGGANIQFGMNPVNGDMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     ITNDITGVTKASFEPTIDYVVVKIPRFAFQKFPGADPTLTTQMKSVGEVMAIGRTFKE
                     SLQKAIRSMEVDQFGFSSKMGLDLGVPPSLNREEAAEQVRKAVRTPLPDRLWRLADGM
                     RLGMTNQELFALTKIDPWFLEQIREIVEFEPRIVAERGALGDAGLRPELLVEAKQLGF
                     ADERLAQLLGVPQSTVRRWRLALGQGNTPRSVTYKRVDTCAAEFEAHTPYLYSTYERE
                     CEARPTTRKKVVILGGGPNRIGQGIEFDYCCVHAAMALREEQVETIMVNCNPETVSTD
                     YDTSDRLYFEPLTEEDVLNIVEREQPMGVVLQFGGQTPLKLALSLSRAGVTILGTSPD
                     AIDRAEDRARFRELLDKLGLRQAESGMAHSVDEALKIAAEITYPVMVRPSYVLGGRSM
                     QIVYDEAGLLHYMNSAVKASAQHPVLIDKYLRDAIEIDADAISDGTTVVVAGIMEHIE
                     EAGVHSGDSACSLPPYTLDSATIEEIRRQMTALALELGVIGLMNAQFAVKDQTIYVLE
                     VNPRGSRTVPFVSKAIGVPLAKLAMKVMVGKSLQQLNFTTAPMPTHLSVKEAVFPFTK
                     FAGVDVLLGPEMKSTGEVMGIDSDFGWAFVKSQAGAGAILPTSGTAFLSVKSEDRAVA
                     CDVAQRLVALGFRITATSGTAAYLTEQGMQVDVVNKVQEGRPHIVDHIKNGDVALVVN
                     TVRTASGHSDSLSIRREALHKGVPYYTTMRGALAAVMGIEALLKKGLAIRALQEYHRV
                     N"
BASE COUNT          773 a         1065 c         1062 g          645 t
ORIGIN      
        1 atcccgtccg gtccgatcca accctaccgg cctccggtgg ggcccatgcc cgaggcgcct
       61 ctgccgaccg gctaacttct tttattcgag gtcaattgtg ccacgacgga cagacatccg
      121 ctccattctc ttgatcggct ccggccccat cgtgatcggg caagcctgcg aattcgatta
      181 ttcgggaacc caggcgtgca aggcgctcaa ggaagagggc taccgcgtca tcctgatcaa
      241 cagcaatccg gccacgatca tgaccgatcc ggattttgcc gaccggacct acatcgaacc
      301 gatcacgctc gacgtggtgg agaaggtcat cgagtacgag cggcccgatg cgctgctccc
      361 gaccatgggc gggcagacgg cgttgaatac cgccatcggt ctggccaaac ggggcgtcct
      421 ggaaaaatac aacgtgaagc tgatcggagc gtcgatcgag gcgatccata aagccgaaga
      481 tcgtgacgca ttccggcagg cgatgtggaa gatcggcctg cgcgtaccgg atagcggcgt
      541 cgcgacatcg ctccaagaag ccgaagaagt ggtccgccgc acgcagttcc cggcgattgt
      601 acggccctcc tttaccatgg gcggaaccgg cggcaacatc gcctacaaca ttgaagagtt
      661 ccggacacag gtggaatggg gcctggccat gagcccggtc cgccaggtgc tcgtcgaaca
      721 gtccgtcatc ggctggaaag agttcgagct cgaggtgatg cgcgacctga aagacaatgt
      781 ggtcatcatc tgtccgatcg agaatctcga cccgatgggc gtccacaccg gggacagcat
      841 caccgtggcg ccggcgctca cgctgaccga caaggaatac cagatgctgc gcgatgcggc
      901 cgtgcgcatc atccgtgaaa tcggtgtgga tacgggcggt gccaatattc aattcggcat
      961 gaatccggtg aacggggaca tggtcgtcat cgaaatgaat ccgcgcgtct cccgcagctc
     1021 ggcgctggcc tccaaagcga cgggattccc catcgcaaaa attgccgcca agctggccgt
     1081 cgggtacacg ctggatgaga tcacgaacga catcaccggc gtgacgaaag cctccttcga
     1141 accgacgatc gattacgtcg tcgtgaaaat tccccgcttt gccttccaga aatttcccgg
     1201 cgccgatccc acgttgacca cccagatgaa atcggtcggc gaagtcatgg ccatcggacg
     1261 cacgttcaaa gaatcgcttc agaaggcaat ccgttcgatg gaagtcgatc agttcggatt
     1321 cagctcgaag atgggcctcg acctcggggt gcccccgtcg ctcaatcgcg aggaagcggc
     1381 ggaacaggtc cgcaaggccg tgcgcacacc gctcccggac cggctctggc gcctggccga
     1441 cggcatgcgt cttggtatga ccaatcagga actctttgcg ctcaccaaaa tcgatccctg
     1501 gtttctcgaa cagattcgtg aaattgtcga attcgaaccg cgaatcgtgg cggaacgtgg
     1561 cgcactcggc gacgcaggac ttcgcccgga actgttggtc gaagcgaaac agttgggctt
     1621 cgctgacgaa cgcctggcgc aattgctggg ggttccacaa agtacggttc gacggtggcg
     1681 cctggcccta ggccagggga acactcctcg cagcgtgaca tacaagcgag tcgatacctg
     1741 cgccgctgaa tttgaagccc acacgccgta cctctattcc acctacgagc gcgaatgcga
     1801 ggcgcggccg accaccagga agaaggtggt gattctgggg ggcggaccaa accggatcgg
     1861 gcagggcatc gaattcgact actgctgcgt gcatgccgct atggcgttgc gcgaagaaca
     1921 ggtcgaaacc atcatggtca attgcaatcc ggaaacggtc agcaccgact atgacacctc
     1981 agaccggctc tacttcgaac cgctcacgga agaagatgtc ctcaatatcg tggaacggga
     2041 acagcccatg ggcgtggtgc tccaattcgg cggacaaaca ccgctgaaac tggcgctctc
     2101 cctgtcgcgc gccggcgtca cgattctcgg caccagcccg gatgccatcg accgcgcgga
     2161 ggatcgtgca cgtttccgcg aactgctcga caaactcggc ttgcgtcagg cggagagcgg
     2221 catggcccac tctgtcgacg aagcactgaa aatcgcggct gagattactt atcccgtaat
     2281 ggtgcggccg tcctatgttc tgggcgggcg atcgatgcag atcgtctacg acgaggcggg
     2341 cctcttgcac tatatgaatt ccgcagtcaa agcctcggca cagcatccgg tactgatcga
     2401 caagtatttg cgcgatgcca tcgaaatcga tgccgacgcg atttccgacg gcacgacggt
     2461 cgtcgtggcc ggcatcatgg aacatatcga ggaagcgggt gtgcactcag gcgactccgc
     2521 ctgttcgttg cccccctata cactggattc cgcgacgatt gaggaaatcc gccggcaaat
     2581 gaccgcgctg gctttggaac tcggcgtcat cggactcatg aatgcgcagt tcgccgtaaa
     2641 agatcagacc atctacgtct tggaggtcaa tccgcgcggc tcgcgcaccg tgcccttcgt
     2701 cagcaaggct atcggcgtcc ccctggccaa actcgcgatg aaagtcatgg tcggcaagtc
     2761 gctccagcaa ttgaacttca ccacagcgcc gatgccgacg catctctcgg tcaaggaagc
     2821 cgtcttcccc ttcaccaaat ttgcgggggt cgacgtgctg ctcggaccgg aaatgaagtc
     2881 caccggggaa gtcatgggca tcgatagcga tttcggctgg gctttcgtca aatcccaagc
     2941 cggcgcgggt gccattctgc cgacttcagg caccgccttt ctcagtgtca aaagtgaaga
     3001 ccgcgctgtg gcctgcgatg tcgcccaacg gctggtcgcg ctgggtttcc gtatcacggc
     3061 gacctccggc accgcggcat atctgaccga gcaggggatg caggtcgacg tcgtcaacaa
     3121 ggtgcaggaa gggcggccgc acatcgtcga ccatatcaag aacggagacg tggccctggt
     3181 cgtcaacacc gtacgcaccg cgtccgggca ttccgactcg ctgtctattc gtcgtgaggc
     3241 gctgcacaaa ggcgtgccgt actacaccac gatgcgcgga gccctggcag cggtgatggg
     3301 gatcgaagcg ttgctgaaaa aggggcttgc cattcgagca ttgcaggagt atcaccgggt
     3361 gaattgagcg aaggtcctga cagagggaca attgacaaca ggttccagga acggacatga
     3421 gtccgccgca cggttcctgg atgcgggcaa ccggacgata ccgaaaggtc caaccggaag
     3481 ccggtttcaa tggtcatcgg ggggaagata tgccgacacc gatcacgaga aaaggatacg
     3541 aagcg
//
LOCUS       DAOQEW010000308         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12416_length_3531_cov_9.403049, whole genome shotgun sequence.
ACCESSION   DAOQEW010000308 DAOQEW010000000
VERSION     DAOQEW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12416_length_3531_cov_9.403049"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PLS35_19115"
     CDS             complement(<1..1331)
                     /locus_tag="PLS35_19115"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20839.1"
                     /translation="MLDPPLQQPSAMIPAPSPAYVDVVLPRRLHRPFTYIIPSELKGR
                     VAIGRSVVVPFGSQDLHGLVIAVHHRLPPGAPQKGLKAIRSLSAASADHLLTAGQIGL
                     SHWVAERYAAPWGQCIKLVLPPVDRVGASQPRYMATAQGLATNASVEPLGETETELLA
                     RLRRRPKGIMATTLLKGDRSRTTLALQALIQKGLVSRCEEPAAQKSVRPAKQQGQVSG
                     GGLPMESVEAVENFELPHAEAMSWPTVLEQALAKKVFVSLLLEGSHCTRQWCLTQAVR
                     ATLRLGRQALILTGDVEHASRLADSFAAEGEQPMLLHSGLSMTARAAVWQAAQEGSVR
                     ILIGTRMAVFAPLRRLGLVWVDGEDDASFKEEQVPRYHAREVAHYRASRDGAVLVLAS
                     NHPSLESLSAVQEGRMTACKYRDAGELPAVHVIDMKEYPRDASGGTVLSPPL"
     gene            complement(1493..3052)
                     /locus_tag="PLS35_19120"
     CDS             complement(1493..3052)
                     /locus_tag="PLS35_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNI20840.1"
                     /translation="MRTLSMRASLTILSCSLMAVTAIPVYAQNAQSVRLGEAALTYAA
                     GSIRQDMPIEGVINVITGDNQLSGNRMMLGWSGTDTLYLKLKIPGDAALGDLYTVYRR
                     SRKVFHPITKQYLGYVINRVGVVKVVQLDPVLVGVQVVRSYAPLSPGDPVMRFTPPAA
                     EEVSEPVSSGPDVEGMIVELQADKHMSLVSQGNVVYLDKGRDDGLRSGDSMEVFRTGG
                     GLPERKIGEVRVLATEPHTATAVLSKASARALVGDRLRSKHGVPVEAMQPDSGEIDRQ
                     AATVQPVMVAKTDASVREANGGRLPATGKIERTRGSRRLNLDDLADQLEYESGEVKVK
                     PAGVPILEKLTEYLTTTATGQQVRVEGHSDNMEIGPSLKGVFPTNWELSKARAAEIVR
                     YLVEKGGMDSAKLSAVGYGASRPVASNGTEEGRKRNRRIEVVLESLEEAAPAQPVKAS
                     VDETASVSTQFTYNQMNAVPVNPTSVPVSEVPSGPAPSDVTPERGPIAPEAGVPASPA
                     DGMPPSPAPGS"
BASE COUNT          768 a         1098 c          988 g          677 t
ORIGIN      
        1 agaggaggcg ataacaccgt ccctcctgag gcgtctctgg gatactcctt catgtcgatg
       61 acgtgcacag ccggcagttc gcccgcatcg cgatacttac atgccgtcat gcgtccttcc
      121 tgaaccgccg acagactctc gagagatgga tgattcgagg caagcacgag caccgcgccg
      181 tcgcgggaag cccgatagtg agccacctct cgggcatgat accggggcac ctgctcttct
      241 ttgaaagacg catcatcctc accatccacc cacacaaggc ctagccgcct caggggagcg
      301 aagaccgcca tgcgggtgcc aatcagaatc ctgaccgacc cctcttgcgc cgcctgccag
      361 accgccgccc gcgctgtcat cgatagacca ctgtgcagga gcatcggctg ttcgccttct
      421 gcggcgaagg aatcagctaa acgactcgca tgctcgacat cacccgtgag aatcaacgcc
      481 tgcctcccga ggcgaagggt ggctcgaacc gcttgcgtca gacaccactg tctggtgcag
      541 tggctaccct ccaggagcaa ggagacgaag accttctttg ccaaggcctg ttccagaacc
      601 gttggccagg acatggcttc cgcatgaggc agctcgaaat tctcaacagc ctccaccgac
      661 tccataggaa gtccgcctcc tgacacctgc ccctgttgtt ttgcaggacg gacgcttttc
      721 tgggcggccg gttcttcaca acgactcacc agcccctttt ggatcagtgc ctgcaaggca
      781 agggtcgttc gggacctgtc gcccttcaga agagtcgtcg ccatgatgcc cttgggacgc
      841 cggcgtaagc gggcgagcaa ctctgtttcg gtttctccca acggctcgac ggacgcgttc
      901 gtcgcaaggc cctgcgctgt cgccatatat cgcggctggg acgcccccac tcgatcgaca
      961 gggggcagaa cgagcttgat acattgcccc cacggtgcgg cataccgttc cgcaacccag
     1021 tggctgagcc cgatttggcc ggccgtaagc agatgatccg ctgaggccgc actcaacgaa
     1081 cgaatagctt tgaggccctt ctgtggggcg ccaggtggaa gccgatgatg caccgcaatg
     1141 acaaggccgt gaaggtcttg cgagccaaat ggcaccacca ccgaacgtcc gatggcgacg
     1201 cggcccttga gttctgaggg gatgatatag gtgaacggtc gatgaagccg acgaggcaat
     1261 acgacatcaa catacgcagg agaaggagcc ggaatcatcg cacttggctg ctgcaaagga
     1321 gggtcgagca tgcggcattc taacaacacc ggcccggtgc aacaatgaca accgggatca
     1381 gctcgcgcga ggaatgagcc cgaacaggta atgagatcag ataaggagaa aggttataga
     1441 gagggcactg aacggcggcc ggcctcttgc gagagaccag ccgccgcctc tactaggagc
     1501 caggggcagg cgacggtggc atcccatcag cgggagaagc cggcactccg gcctcagggg
     1561 ctatcgggcc acgctcgggt gtcacatccg agggagccgg tccagacggc acctccgaca
     1621 ccgggacgct cgtcggattc accggcacgg cattcatctg gttatacgta aactgtgtcg
     1681 acaccgatgc cgtttcatca accgaggctt tcaccggttg cgctggggcc gcctcttcga
     1741 gggactccag tacgacttca atgcggcggt ttctcttgcg tccctcttcg gtcccgttgc
     1801 tcgccaccgg ccggctggct ccatatccga cggcagacag cttcgcagaa tccattccac
     1861 ccttctcgac caaataccgg acgatctctg ccgcccgggc cttcgataat tcccaattgg
     1921 tgggaaagac gcctttcaag gacgggccga tttccatgtt atcggaatga ccttcaacgc
     1981 gaacctgctg accggtggcc gtagtggtca aatactcagt caatttttca aggatgggta
     2041 ccccggcggg cttcactttg acttccccgg actcgtattc caactggtcg gccagatcgt
     2101 ccaggttgag tcgccgactt ccacgagtac gttcgatttt ccctgtggcg ggcagacggc
     2161 ctccattcgc ctcacggacg gaagcatccg tcttcgccac catcacaggc tgtacggttg
     2221 ctgcctgacg atcaatctct ccgctgtcag gctgcatggc ctcgacgggc acgccatgtt
     2281 tggaacgaag acgatctccg acgagggcgc gggccgaagc cttggacaag accgcggtgg
     2341 ccgtatgagg ttccgtggca agcactctca cttcgccgat ctttcgctcg ggcaagccgc
     2401 cgcccgtgcg aaacacctcc atagagtctc cggaccggag accatcgtcg cgccccttgt
     2461 cgaggtacac cacattgccc tgcgacacga gtgacatatg cttgtcagcc tgtaactcaa
     2521 cgatcatccc ttccacatct ggccctgaag aaacaggctc gctgacttcc tccgcggccg
     2581 gaggcgtaaa gcgcatgacg ggatcaccgg gtgaaagtgg tgcgtaagac cgaacaacct
     2641 gcacgcccac gagcaccgga tccagctgca cgactttcac aacaccgacc cgattgatga
     2701 catatcccag atactgcttg gtgatgggat ggaacacctt gcgagaccgc cgatatacgg
     2761 tgtagaggtc accgagcgcc gcgtcgcctg ggattttcag tttgagatac agcgtgtccg
     2821 tcccggacca gcccaacatc attcgattcc cggacaattg attgtcgcca gtaatgacat
     2881 tgatgacacc ctcgatcggc atgtcttgcc tgatcgaccc ggcggcatag gtcaaggctg
     2941 cctcacccag tcgcacgctt tgcgcattct gagcgtacac tgggatggcc gttacagcca
     3001 tgagactgca tgagaggatg gtcaggctgg cgcgcataga taatgtcctc atattgatca
     3061 cacctaggag ggttgcctgt agcaacctta gcaaagagca gagggttgtc aagaaaccgc
     3121 gcacaggcca agggtttgaa tcaggccact ctggatgtta tcgtgagatc ggaaccgaat
     3181 acgtcccgga accactatcg agcgggtgac caacgcaatg gacaaacgac gcatcaacac
     3241 tcagatgccc cgccgaacca aggttcaacc atcgacgctc atggcatctg agtgggatat
     3301 cggcgccgga tctcctccgg ctggtttgag aactccggcc agccagatga agaccgcctc
     3361 cacgcaagag cattcgatca gcgatgtttc agcgacatcg cgcaagaaga catcaagcaa
     3421 gccccgcgaa gcgtgacaat cgacgcgcgc tcctaactcc tggacagtat tcactttcta
     3481 ggactatcgg gactaaagcc ctaggtcctt tgtgcgcttg ctattaaaaa t
//
LOCUS       DAOQEW010000309         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12555_length_3506_cov_7.173573, whole genome shotgun sequence.
ACCESSION   DAOQEW010000309 DAOQEW010000000
VERSION     DAOQEW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_3506_cov_7.173573"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..>1144)
                     /locus_tag="PLS35_19125"
     CDS             complement(365..>1144)
                     /locus_tag="PLS35_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20841.1"
                     /translation="DWNEDEEEDDWELVEDERLAVTDALISPLLIDGLTNAAAKGNAT
                     AHFALALIHADDDIDDVETGSDYWYQQAQSGRVLAGVEKEWADSHEARLNREQLFARH
                     LKEAARLGCPEALLELADRFDDPAFFEQATSDVNADPAWVAEIAERLGRREDSKKWLT
                     EAAKCGDTEAMRQLIEEFDHGDLVRCWTWLYLAEMLGSDLTKDDYHAIHEDGSPYDDD
                     VGGPIFADGRDGVRLEPISADQQATARQNAAEMFRVIRDDE"
     assembly_gap    1145..1244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1245..1456
                     /locus_tag="PLS35_19130"
     CDS             <1245..1456
                     /locus_tag="PLS35_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20842.1"
                     /translation="PTTRLKERFAMTEQIACFYSCASKVGTASNFSFEAEIRPCQKDV
                     EARAYSEMFSELQKMAMSPWIRMRA"
     gene            complement(1440..1676)
                     /locus_tag="PLS35_19135"
     CDS             complement(1440..1676)
                     /locus_tag="PLS35_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20843.1"
                     /translation="MSKTPVPTPQLNRLRAASALIPIIQSGLANAKLSRDRAAQMTQF
                     VEWTTHCTPEDATEQALVDEVTAGLHRLQATLAS"
     gene            1858..2274
                     /gene="umuD"
                     /locus_tag="PLS35_19140"
     CDS             1858..2274
                     /gene="umuD"
                     /locus_tag="PLS35_19140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNI20844.1"
                     /translation="MAQDPVAFEFRLLTHRISAGFPSPAADYAEEGLDLNDYLVRNKP
                     ATFMFTVRGDSMIGASIEEGDKVVVDRAMTPKHNDIVVAVVEGEYTIKRLYKHQGRIE
                     LRPENPAYSPIIFNDGVELLVWGVVVGVVRRYSSRG"
     gene            2277..>3506
                     /locus_tag="PLS35_19145"
     CDS             2277..>3506
                     /locus_tag="PLS35_19145"
                     /inference="COORDINATES: protein motif:HMM:NF002955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNI20845.1"
                     /translation="MTSAPQFALVDVNNFYVSCERVFKPSLENTPVVVLSNNDGCAVA
                     RSNEVKSLGVKMGMPWFKMKDLARQHGIQAYSSNYALYGDMSNRVTSILRDYSPDLEV
                     YSIDESFLRIETVAHLYGGAIPMGQQMRHRIKQWTGLPVCVGVGPTKTLAKFANHLAK
                     KNDVFNGVCDLHAMTRPERLEWMWGVEVGEVWGVGSRIRKRLAGMGIDTVLDLRNASP
                     KQMRAQFGVVMERTCNELRGISCLELEDVMPPKQQIMSSRSFGAPVETIEELRESVAS
                     YLASAAEKLRRQNSVAGAVYVFVLTNRFKEDEPQYNAGITVPMDDATDDTLALTSAAL
                     RGLAAIFRPGFRYKKAGVMLTLLSDKGARQATLFDDPVARAKSEKLMEAMDAINREFG
                     RGTLRSGASGVAQRWAMR"
BASE COUNT          661 a          960 c         1022 g          763 t
ORIGIN      
        1 cagcggactg aaaatccgcg tgtccgtggt tcgattccgc gcttggccac caaaattcaa
       61 accccaacca ctctcggttg gggtttttta ttgcctgttt gcgccaactc ccgcgtgttc
      121 tcgcgggttc cttcggagtc ttgcggactt cgccttcgcc accgctaggc aggttcggcg
      181 cccggttctc tctctattgg ccctttctct ctgcgggcgg gactttgtgt ttttgcccga
      241 agtccgcaat ggtcacgacc taccccctat aaagatcaat gggttacgag ttgtcgaatc
      301 gactggtttg ttttacagat tggtaggcga gtaattcgtc acccaccaac cccaggcgtt
      361 gctactactc gtcgtccctg atcacacgga acatctccgc tgcgttttgc cgtgcagttg
      421 cctgttgatc tgcactgatc ggttcaagcc tgacgccatc gcggccgtct gcaaagatgg
      481 ggccaccgac gtcatcgtcg taaggggacc catcttcgtg gatagcgtgg tagtcgtcct
      541 tggtcagatc gctaccaagc atttctgcga ggtagagcca tgtccaacaa cgaaccagat
      601 caccatggtc aaactcttca atcagctggc gcatggcttc ggtgtcaccg catttggccg
      661 cctcagtcag ccacttcttc gagtcttcgc gcctgcccag tcgttcggcg atctctgcca
      721 cccaggcggg gtctgcattg acgtcactgg tggcctgctc gaaaaacgca gggtcgtcaa
      781 aacgatcggc caattccagt agcgcttcgg ggcagccaag tctggcggct tccttcaggt
      841 gtcgtgcgaa tagctgctcg cggttcagtc tggcttcatg gctgtctgcc cactctttct
      901 cgacacccgc gagtacacgg ccgctttgcg cctgctggta ccagtagtca ctgccggtct
      961 ctacgtcgtc gatgtcgtcg tcggcgtgga tcagcgccaa tgcaaagtgg gcggtcgcgt
     1021 tacctttggc tgccgcgttg gtgagtccat caatgaggag tggtgaaatg agggcgtcgg
     1081 taaccgccag ccgttcgtct tccacgagct cccagtcatc ttcctcttcg tcctcgttcc
     1141 agtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccga cgacgaggct
     1261 caaagagagg tttgcaatga cggaacagat cgcctgtttt tactcgtgtg caagtaaggt
     1321 gggcaccgca agtaatttta gcttcgaagc cgaaatccgt ccatgccaga aagatgtcga
     1381 ggctagagcg tattcagaaa tgttttctga acttcagaaa atggctatgt cgccgtggat
     1441 caggatgcga gcgtagcctg caatcggtgc agccctgcag tgacttcatc gaccaaggct
     1501 tgctctgtgg cgtcctcggg agtgcagtgg gtggtccatt cgacaaactg tgtcatctgg
     1561 gcagcgcgat cgcgcgagag cttggcgttg gccagccccg actggatgat cgggatgagt
     1621 gccgacgcag cgcgtaagcg gttcagttgc ggggtgggta cgggcgtttt cgacatagcc
     1681 aactattgaa gcatctttgt ctttttaggt aaacgatgcc agatccatgg agagcttgtt
     1741 ggcagcttgt ggccactcag gcgtggcatt tatactgtac ggaaaaacag tatccgtatg
     1801 cagccaactc ttgtccgtcg tcgtccttcg ccatgcctga gccagactcc tttggaggtg
     1861 gcacaggacc cggttgcgtt cgaattccgg ttgctcacgc atcggatcag cgcgggattt
     1921 ccgtcgccgg cggcggacta cgctgaagaa gggctcgatc tcaacgacta cctcgtgcgc
     1981 aacaagccag cgactttcat gttcacggtc aggggcgact ccatgatcgg cgccagcatc
     2041 gaggaaggcg ataaggtggt cgtggaccgt gccatgacgc cgaagcataa cgacatcgtg
     2101 gtcgccgtgg ttgagggcga gtacacgatc aagcgcctct acaagcatca ggggcggatt
     2161 gaactccggc cagaaaatcc cgcctacagc ccgatcatct tcaacgacgg cgtggaactg
     2221 cttgtctggg gtgtcgtggt tggcgtggtc cggcgctata gctcccgagg ttgaccatga
     2281 cgtccgcgcc gcagtttgcg ctggtggacg tcaataactt ctacgtctcc tgcgaacgcg
     2341 ttttcaagcc cagtctggaa aacaccccgg ttgtcgttct ttcgaacaac gacggttgtg
     2401 ctgttgcgcg cagcaacgag gtcaaatcgc tgggcgtgaa gatggggatg ccatggttca
     2461 agatgaagga cctggcccgc cagcacggca tccaggccta ctcatcgaac tatgcgctgt
     2521 atggcgacat gagcaatcgg gtgaccagca tcctgcgcga ctattctccg gacctggagg
     2581 tctacagcat cgacgagtcc ttcctgcgca tcgagacggt cgcccacctt tatggtggtg
     2641 ccatccccat ggggcaacag atgcgtcacc gcatcaagca atggacgggc ttgcctgtgt
     2701 gcgttggtgt cgggccgacc aagacgctgg cgaagttcgc caaccacctg gccaagaaga
     2761 acgacgtttt caacggcgtg tgcgacctcc atgcgatgac gcggccggaa cgtctggagt
     2821 ggatgtgggg tgtcgaagtt ggcgaggtat ggggcgttgg gtcccgtatc agaaagcgcc
     2881 tggctggcat gggcatcgac accgttctcg acctacgcaa tgcctcgccc aagcaaatgc
     2941 gagcccagtt cggggtggtc atggaacgca cctgcaacga actgcgcggg atttcctgcc
     3001 tggagttgga ggatgtcatg cctcccaagc agcagatcat gtcgtcgcgc agttttgggg
     3061 cgccagttga aaccatcgag gagttgcgtg agtcggtggc aagctacctg gccagtgccg
     3121 cagaaaaatt gcggcgacag aactccgtag cgggtgccgt ctatgtcttc gtactgacca
     3181 accgattcaa agaggacgag ccgcagtaca acgccgggat cacggtgcca atggacgatg
     3241 ctacggatga cacactggcg ctgacctctg ccgcattgag ggggctggcg gcgattttca
     3301 gaccaggatt tcgctacaag aaggctgggg tgatgctgac cctgctctcc gataagggcg
     3361 cccgccaagc gacgctgttt gatgatccgg ttgcccgagc aaaatcagag aagctgatgg
     3421 aagcgatgga tgccatcaac cgtgagttcg ggagggggac cctccgctcg ggcgcgagtg
     3481 gcgttgcgca gcgttgggcg atgcgc
//
LOCUS       DAOQEW010000310         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12684_length_3485_cov_10.222449, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000310 DAOQEW010000000
VERSION     DAOQEW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12684_length_3485_cov_10.222449"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PLS35_19150"
     CDS             <1..1431
                     /locus_tag="PLS35_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HNI20846.1"
                     /translation="ASLTMAKLIKDLYPDVKIVFGGANFDGEMGLEYFRAFPFIDYVV
                     VGEGEVTFPALVDYVLRGSTGPCPKGVIYREDGEVKLEPNTALFTEFAQTGPPDYDDY
                     YHLLAELGTEASRGLDRILLYEGSRGCWWGEKHHCTFCGLNAQSMKFRAKSAEQVAQE
                     MAYLSSRYDTTRFRLVDNIIDMKYVDNLFGRFAAAHCDLDVFIETKSNLQKSQIRTLA
                     VGGVKCMQPGLESLSLSQLKAMDKGVTPMQNIICLKWSCYYRVAVSWNILLGFPGETN
                     EDYRRQIDLLPSLFHLQAPEATGKFWLQRFSPYFTRPHEYGVRITGPGTAYEYVYDAA
                     RVDLRKIAYDFEYELDDWPVDPQVYQELTGLIEEWQRRARSNDRPFLYYSKAMEYVTI
                     YDGRDPDMPTRQRYDWPAAGIIEACNEAAKSRDQIRAALGEAKRDSIYSEDDLTEALS
                     LLTSRRILYEEREKYFTLAIPEHPYY"
     gene            complement(1608..1940)
                     /locus_tag="PLS35_19155"
     CDS             complement(1608..1940)
                     /locus_tag="PLS35_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNI20847.1"
                     /translation="MDCYYHSKDLGKFGDMGKGNPVLWEKFMSYYSAVFAEGALTERE
                     KALIALGVAQAVQCPYCIDAYTQACLEKGSNIEEMTEAVHVACAIRGGASLVHGVQMR
                     NVAEKLSM"
     gene            complement(2027..2296)
                     /locus_tag="PLS35_19160"
     CDS             complement(2027..2296)
                     /locus_tag="PLS35_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20848.1"
                     /translation="MASQVPPQKPAAPSANAQDVWDVELLHVHVSRKGQLVNAEWALH
                     PQIKADLSAEEWRELGELMNKVTTIVGHRFSQALNEAEPTPPGNA"
     gene            complement(2424..3245)
                     /locus_tag="PLS35_19165"
     CDS             complement(2424..3245)
                     /locus_tag="PLS35_19165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNI20849.1"
                     /translation="MHTSHALPSEELDRYLATAVQAAEAAGHVLLEHAKTGFRIDHKA
                     AINLVTDADRQAEECIVRSILSSHPTHRILAEERGRDGATESPYQWIIDPLDGTTNFA
                     HGFPFYSVSIGLEYHGDCIVGVVLDPTRQELFTGVAGKGAYLNGERLRVSNNETLGRS
                     LLVTGFAYDIRETTNNNLDHFSRISLRAQGVRRTGSAALDLSYVASGRFDGYWEVKLS
                     PWDMAAGIVIVREAGGLVSGFQKDTFSLYGQELVATNGLIHQQLLDVIHQRPATS"
BASE COUNT          763 a          932 c         1015 g          775 t
ORIGIN      
        1 gccagcctca cgatggccaa gctcatcaag gacttgtatc ccgacgtgaa aatcgtgttc
       61 ggtggggcga attttgatgg ggagatgggg ctggaatatt ttcgagcatt tccgttcatc
      121 gactatgtgg tggtcggcga aggagaggtc acgttccctg cattagtcga ctatgtcttg
      181 cgtgggtcga ccggcccctg cccgaaaggc gtcatctacc gggaagacgg tgaggtgaaa
      241 cttgagccga atacggccct gttcacggag tttgcccaga ccggaccgcc ggactatgac
      301 gactactacc acctgctggc ggaactcggc actgaagcct cgcgcggcct ggatcgtatt
      361 cttctatatg aaggttcccg aggctgctgg tggggcgaga aacaccattg caccttttgc
      421 gggctgaatg cgcagagcat gaaattccgg gcgaaatccg ccgaacaggt cgcgcaagag
      481 atggcgtacc tctcgagccg ctacgacacg acccgcttcc ggctggtgga taacatcatc
      541 gacatgaaat atgtcgacaa cctgtttgga cgatttgctg ccgcgcattg cgatctggac
      601 gtgtttatcg agaccaagag caatctgcag aagagccaga tccgcaccct ggccgtgggt
      661 ggcgtgaagt gcatgcagcc cggcctggaa agcctgagtc tttcgcagct gaaagcgatg
      721 gacaagggcg tcacgcccat gcagaacatc atctgcctca agtggagttg ttattatcgg
      781 gtcgcggtgt cctggaatat tctgctcggg tttcccggcg aaacgaatga ggattatcgc
      841 cggcagattg atctcctgcc ctccctattc catttgcaag cgccggaggc caccgggaaa
      901 ttctggctgc agcgcttcag cccctacttt acgcgaccgc atgagtacgg agtgcgcatc
      961 acgggtccgg ggacggccta tgaatatgtg tatgatgcgg cgcgcgtcga tttgcggaag
     1021 atcgcctacg attttgaata tgaactcgac gactggccgg tggatccgca ggtctatcag
     1081 gaattgaccg gactgataga agaatggcag cgccgggctc gcagcaacga ccggccgttc
     1141 ttgtattatt cgaaggccat ggagtatgtg accatctacg acgggcgtga tccggacatg
     1201 ccgacgagac aacgatacga ctggccagcg gcggggatta ttgaggcatg taatgaagcc
     1261 gcgaagagtc gcgatcagat tcgcgccgca ttgggcgagg cgaagcggga cagcatctac
     1321 tcggaagacg atctgactga agcgctgagc ctcttaacca gccgtcgcat cctttacgaa
     1381 gagcgcgaga aatatttcac gctggctatc cccgagcatc cctattactg atgggctggt
     1441 gaggcagaaa gccggcgttg tttcttgcct aagctcagta cctcaaggta caggtctcct
     1501 tcgtctcggt cctgcacgtg ctgcggcctt gctaggcctc ctctttgacc atccactggt
     1561 cccattggct ggcacgtttc tgctgcaaac gagtcgccgc tgaaacatca catcgacagc
     1621 ttctccgcca cattgcgcat ttggacgcca tgcacgagtg acgcaccgcc gcggatcgcg
     1681 caggcaacgt ggacggcttc cgtcatctct tcgatgttcg atcctttttc caggcaggct
     1741 tgagtatagg catcgatgca gtacggacac tgcacggctt gcgccacacc aagtgcaatc
     1801 agtgccttct cccgttccgt gagggcgcct tcggcaaaca cggcgctgta atagctcatg
     1861 aacttttccc acaggacggg attgccctta cccatatctc cgaatttccc aagatccttc
     1921 gagtggtagt agcagtccac ggatcacctc gctggttagt ggtctgatca tgctcgcgcg
     1981 tcagccgatg agcatgcgta gtagcggtct gtgatggcga gaagcgttac gcgtttcctg
     2041 gaggtgtcgg ttcggcctca ttgagggcct gggagaaacg gtgtcccacg atggtcgtga
     2101 ccttgttcat caattcgccg agttctctcc actcctccgc actcaaatcg gctttgatct
     2161 ggggatgaag ggcccattcg gcattcacga gttgcccctt acgcgagaca tgcacatgca
     2221 agagctcaac atcccacaca tcctgggcgt tggcggacgg ggcggctggt ttctgcggag
     2281 gaacttggct tgccatgctg cgtgcactcc tttgaccata cagatgagag ggcatgatac
     2341 cgcatcaaat atcgcactgt acatgggacg agcgacgtga atgccaatcg catcggccgg
     2401 gtcggatatc accgctcagg aggttaggac gtggcgggtc gttgatggat gacgtcgagc
     2461 agttgttgat gaatgaggcc gttggtggcc acgagctcct gcccatagag agaaaatgta
     2521 tctttctgaa agccggatac cagtccgccc gcttcacgca cgatgacgat cccggcggcc
     2581 atatcccaag ggctgagttt cacttcccaa tatccgtcaa accgcccaga ggccacataa
     2641 ctgagatcca gggccgctga gccggtgcgt cgcacgcctt gagcgcgcag cgagatgcgg
     2701 gagaaatggt ccagattgtt attcgtcgtc tcgcgaatat cgtacgcaaa tccagtcacg
     2761 agaagagaac gaccgagggt ctcattgttc gacacgcgga ggcgttcgcc attgaggtag
     2821 gcccctttgc cggcgacacc cgtgaacagt tcctgacgag tcgggtccag gacgacgcct
     2881 acgatacagt cgccatgata ttccagcccg atcgagaccg aataaaacgg gaacccgtgc
     2941 gcgaagttgg tcgtgccgtc gagcggatcg atgatccatt gatatggcga ctcggtggcc
     3001 ccgtcccggc cgcgttcctc ggctaagatc cggtgggtgg gatgagacga aagaattgaa
     3061 cggacaatac attcctcagc ctgccggtcg gcatcggtca cgaggttgat ggcggctttg
     3121 tggtcaattc tgaaccctgt tttggcatgc tcaaggagca cgtggccggc tgcttctgcc
     3181 gcctgaacag ccgtggccag atagcggtcc aattcttcag atgggagtgc atgcgacgta
     3241 tgcatagacg ccgtatccta gcatgagtgg tgtaaagcgg ggtaaaaagc cttggtcatt
     3301 ttgagagagc aaattaacat attgaaaata aatgatttaa taaaagcttg ttcttgcaga
     3361 acaaggcttg gctaggcact gagtgcattc tgcttgcata ggcgataagc gcgaacaaat
     3421 agttatgatc agttacttga cagattttga atcacttgct ataaagcaag tatgctttat
     3481 tgagg
//
LOCUS       DAOQEW010000311         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12783_length_3466_cov_10.136910, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000311 DAOQEW010000000
VERSION     DAOQEW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12783_length_3466_cov_10.136910"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLS35_19170"
     CDS             complement(<1..979)
                     /locus_tag="PLS35_19170"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNI20850.1"
                     /translation="MSKLIFVTGGVVSSLGKGLASASIGNLLESRGLKITFLKLDPYI
                     NVDPGTMNPYQHGEVYVTEDGAETDLDLGHYERYTSLTLTRENNYTTGRIYHSVITKE
                     RRGDYLGGTVQVVPHVTDEIKQCIMRTSQGMDVTIVEIGGTVGDIESLPFLEAIRQLP
                     YDVGRDNVLYVHLTLVPYIGAAGELKTKPTQHSVNKLREIGIQPNILLCRTDRYLPPE
                     LKAKIAMFCNVEKDAVITAKDVETIYEVPIVFRKEGLDELIVRQLKLETGPPNLREWD
                     AMVQKIKHPKHEVSIALVGKYAGLKECYKSLAEALVHGGIDHETRVNVTW"
     gene            complement(1011..1781)
                     /gene="kdsB"
                     /locus_tag="PLS35_19175"
     CDS             complement(1011..1781)
                     /gene="kdsB"
                     /locus_tag="PLS35_19175"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNI20851.1"
                     /translation="MKRSVTVVIPARYGSSRFPGKPLIDLMGKPMIQHVYEQAKACRV
                     VDDVLVATDDDRIKRAVEGFGGQVLMMTEPYRTGTDRVAAVAKSRGGDCYIDLQGDEI
                     LLHPDLITDLVEPFLASDAPMGTLKRRIDSDQDLQNTGVVKVTTDREGNALYFSRAPI
                     PLVRDDPKRSAVPGLHFIHLGLYIYTRETLASLTALPTGILEDAEKLEQLRALENGIR
                     IRVWETSQMSLRIDSPEDVPVALTQLRAHTSRAGSTQP"
     gene            complement(1783..2847)
                     /gene="rfaE1"
                     /locus_tag="PLS35_19180"
     CDS             complement(1783..2847)
                     /gene="rfaE1"
                     /locus_tag="PLS35_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNI20852.1"
                     /translation="MGKVRKSHPVGRSGHAGPSNGHAAEEAAMDHAGLGAYIQRFSRA
                     SVLVIGDLILDHYVWGRVSRISPEAPVPVVHVESESLKLGGAANVFNNILALGGQADI
                     CGVIGADESGRLLLKELGGRRQGRGGVVIDQSRPTTRKSRVVAHNQQIVRYDVERRNE
                     LSSLLQRRILRYVESRLKELSCLVVSDYAKGVVTASLMTELTRLAGQRKIPIVVDPKV
                     EHFSYYKGVTVVTPNHLEATQAAGVQGEDDKTINKAGMVLRQRLGCQTVLVTRGERGM
                     SVYQGHGDHWHIPTRARQVYDVTGAGDTVVGTLALALSTGASMREAAILANQAAGVVV
                     GMVGTATVTAAQLADALGQS"
     gene            complement(2817..>3466)
                     /gene="lepB"
                     /locus_tag="PLS35_19185"
     CDS             complement(2817..>3466)
                     /gene="lepB"
                     /locus_tag="PLS35_19185"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNI20853.1"
                     /translation="ILREYAEAIIIAMLLAFAIRVFVVQAFKIPSGSMIPTLLVGDHI
                     LVSKLSYGLQWPTDCKFQPGFPPVTCYSSHTVIPFGSIQRGDIIVFRFPEDEDKDFIK
                     RVIGLPGDTIHVRNKVVHINGTPFDDHAFTQHTDPPIHDGHISPRDNFGPVTVPEDAY
                     FVMGDNRDHSLDSRFWGYVRTEKVRGKAFRIYWSWSGQGSWTEWVRWERLGKAIQ"
BASE COUNT          648 a         1050 c         1004 g          764 t
ORIGIN      
        1 tccacgtgac gttcacgcgg gtttcgtggt cgatcccgcc gtgcaccagg gcctcagcca
       61 ggctcttgta acattccttc agtccggcat attttccgac gagcgcgatc gacacttcgt
      121 gtttgggatg cttgatcttc tgcaccatcg cgtcccattc gcgcagattg ggcgggccgg
      181 tttcgagctt cagctgccgg acgatcagct catccagccc ttccttgcgg aacacgatcg
      241 gcacttcgta gatggtttcc acatccttgg ccgtaatgac cgcgtccttt tccacgttgc
      301 agaacatggc gatcttggct ttgagctcgg gtggaaggta gcgatccgtg cggcataaca
      361 ggatgttcgg ttgaataccg atttcgcgca gcttgttgac ggaatgctgg gtcggtttgg
      421 ttttcaactc acctgcggcg ccgatgtacg gaaccagggt caggtgcaca tagagcacgt
      481 tgtcgcgtcc cacgtcatag ggaagttgcc gaatggcttc cagaaacggc aggctttcga
      541 tatcgccgac cgtgccgccg atttccacga tcgtgacgtc cataccttgc gacgtccgca
      601 tgatgcattg cttgatctca tcggtcacat ggggaaccac ctggaccgtt ccgcccaaat
      661 agtcaccgcg ccgctctttt gtgatgactg agtgataaat acggccggtg gtgtagttgt
      721 tttcgcgcgt cagcgtgagc gaggtgtatc gttcgtaatg ccccaggtcg agatcggtct
      781 cggccccgtc ttccgtcacg taaacctcgc cgtgctggta gggattcatg gtgcccgggt
      841 cgacgttgat gtaggggtcg agtttcaaaa aggtgatttt caacccgcgg ctttccagga
      901 gattgccgat ggacgcggaa gccagtccct ttccgagcga cgatacgaca cccccggtca
      961 caaagatcag cttgctcatc gtggcacctt cttgtgaatg cggccaatag tcatggttga
     1021 gtgcttcccg ctctgctcgt atgagcccga agttgggtca aggcgaccgg cacgtcctcg
     1081 gggctatcga tgcgtaagga catctgggag gtttcccaga cgcgaatcct gattccgttc
     1141 tccagcgcgc gcagctgctc gagtttctcc gcgtcttcca ggatcccggt cggtaatgcc
     1201 gtcaggctcg cgagggtctc gcgtgtatag atatacagac ccaaatgaat gaaatgtaat
     1261 cctgggacgg ccgaccgttt cggatcgtca cgaaccagcg ggatcggtgc ccgggaaaag
     1321 tagagagcat tgccttcccg gtctgtcgtg actttcacca caccggtatt ctgcaagtcc
     1381 tggtcggagt cgatccgccg tttgagtgtg cccatcggcg cgtcgctggc caggaatggt
     1441 tcgaccaggt ccgtgatcag gtcgggatgc agcagaattt catccccctg caggtcgatg
     1501 tagcaatcgc cgccacgaga cttggcaacg gctgccacgc gatcggtgcc ggtgcgatag
     1561 ggctccgtca tcatgaggac ctggcctccg aatccctcga cggccctctt gatacggtcg
     1621 tcgtctgtgg cgaccagcac gtcatccacc acgcggcagg cctttgcctg ctcatagaca
     1681 tgttggatca tcggcttgcc catcaagtcg atcaacggtt tccccggaaa ccgcgaggat
     1741 ccgtaccggg ctggaatcac caccgtaacc gaacgtttca cctcagctct gtcccagggc
     1801 atccgccaat tgggcggcgg tcaccgtggc ggtgccgacc atgcccacca caaccccggc
     1861 agcttgattc gcgaggatgg ccgcctcccg catgctggct ccggtcgaga gcgccagcgc
     1921 cagcgttccg accaccgtgt cgccggcacc ggtcacatca tagacctgcc tggcgcgggt
     1981 gggaatatgc caatggtcgc cgtgcccctg gtagacactc atccctcgct cgcctctggt
     2041 gaccagaaca gtctggcatc ccaaacgttg gcgaaggacc atcccggctt tattgatggt
     2101 cttgtcatct tcgccttgca cgccggccgc ctgtgtggct tccaaatggt tgggggtcac
     2161 gacggtcacg cccttgtaat aactgaaatg ctccaccttg ggatcgacga caatggggat
     2221 cttgcgctgt cccgccaaac gagtcaactc tgtcatcagc gaggccgtga cgacgccctt
     2281 ggcatagtcg gagaccacca gacaggagag ttccttcagg cgtgattcca cgtaccggag
     2341 aatacgccgc tgcaggaggc tggagagttc gttgcggcgt tcgacgtcat agcgaacgat
     2401 ttgctgattg tgggcgacga cgcgtgattt tctggtcgtc ggccggcttt gatcgatgac
     2461 gactccgccc cgtccctgcc tgcgcccgcc caattctttc aggaggagcc gtccgctttc
     2521 gtccgccccg atgactccgc agatatccgc ctggccaccg agtgccagaa tgttgttgaa
     2581 gacgttcgcc gcgccgccga gcttcagtga ttccgattcg acatgcacca ccggcaccgg
     2641 agcttccgga gagatccggc tgacccggcc ccagacgtag tgatccagga tcaaatcccc
     2701 gatgaccagc acactggccc gggaaaatcg ctggatgtac gcgccgagtc cagcgtgatc
     2761 catcgccgcc tcttctgccg cgtgcccgtt cgacggtccg gcatggccgg atcgccctac
     2821 tggatggctt ttcctaacct ttcccatcgt acccattccg tccacgatcc ttggccgctc
     2881 cacgaccagt aaatgcgaaa tgctttgccg cgcactttct ccgtgcgcac gtatccccag
     2941 aaccgactgt cgagactgtg atcccggttg tcccccatca cgaagtaggc atcctccggc
     3001 accgtcaccg ggccgaaatt gtctcgcgga ctgatgtgcc cgtcgtgaat gggcggatcg
     3061 gtgtgctgcg tgaaggcatg gtcatcgaac ggggttccgt tgatgtgcac caccttgttg
     3121 cgtacgtgga tggtgtcacc cggcagcccg atcacacgct tgatgaagtc cttgtcttcg
     3181 tcctcgggga accgaaacac gatgatgtcg ccgcgttgaa tcgatccgaa cggaatgacc
     3241 gtgtgcgagg aatagcaggt gactggtgga aagcccggtt gaaacttgca gtccgtcggc
     3301 cactggagac cgtaggataa tttgctgacg aggatatggt ctcccaccag aagcgtggga
     3361 atcatcgagc cggatggaat tttgaaggcc tgcaccacga agacacggat ggcgaacgcg
     3421 agcaacatcg caatgatgat ggcttccgca tattcgcgca ggatcg
//
LOCUS       DAOQEW010000312         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12811_length_3462_cov_7.636924, whole genome shotgun sequence.
ACCESSION   DAOQEW010000312 DAOQEW010000000
VERSION     DAOQEW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12811_length_3462_cov_7.636924"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PLS35_19190"
     CDS             complement(<1..1466)
                     /locus_tag="PLS35_19190"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNI20854.1"
                     /translation="MISSHTEALRPAVKTEPIPPHIVEEIETFEAEAQRVLAGDLSTD
                     IFKPFRLQYGIYGQRQPGVQMFRIKIPFGGLTANQLRRVAELADQFATGVGHVTTRQD
                     IQLHFVELKHVPEMMRQLAAVGLTTREACANTVRNVTACHLAGVCQGEVFDVTPYAKT
                     VAYHLLRNPLNQSLPRKFKIALSGCRQDCALTPIHDIGLLAAKRADGTIGFRMVAGGG
                     LGSTPRMAQVLREFTPMDELLPTIEAVIKVFDTLGNRKNRNKARMKFVIEKLGFDEFK
                     RRWEAAYAAMGYAVPTHEPIKLLEYADTPPLLMPTKAPNSTNGNGNGNGNGAASRNGA
                     ESAFEAWKRTNVVPQRQAGFAAAAIKLPMGDLTGEQMWVVADLAERYSNGNIRTTINQ
                     NVIIRWIQEDRLSAFHEELVAHSLGDPGAELVEDIIACPGTDTCGLGITSSKGMARAL
                     AEVFPAGQVPEDLRDVSVKISGCHNSCAQHHIATIGLHG"
     gene            complement(1516..1968)
                     /locus_tag="PLS35_19195"
     CDS             complement(1516..1968)
                     /locus_tag="PLS35_19195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNI20855.1"
                     /translation="MKVSLRATYGIIAAVDLALHHAEHPVCAKSIAKRQAIPARFLEQ
                     VLHAMKKAGVVTSQRGAQGGYVLSRKPSELSVADILDALEGPLLSTNGDAAPKQTSSR
                     ASKQEALLAHIWDRVKRAELSVLSEVTVEELAKRQRALDEQHTLMYHI"
     gene            1983..2330
                     /locus_tag="PLS35_19200"
     CDS             1983..2330
                     /locus_tag="PLS35_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20856.1"
                     /translation="MGIHECLYIEPRFPVKPAGAGLVADIADALAAITLADNCSPSLG
                     RDEFAASKVSNLHCELMPPTISSTSAHFLHFDRSGCINTGADFLIDIIWGLSNNPALF
                     FLGASHDAAFINI"
     gene            2305..2889
                     /locus_tag="PLS35_19205"
     CDS             2305..2889
                     /locus_tag="PLS35_19205"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI20857.1"
                     /translation="MTLRSSTFNILLLSTDSEIQSHYKDLFGEQAITVGREGPSLQKD
                     LAKRDYDAVIIESKLAAAADLSKLLTGIDPSRTLLLVGSRTVLKRTAAVLQASKASKA
                     EAPPSNGKGQALCLDSYLEHKVGDFVKGMRNGSGKNLHPMLIAAVERPLILLTLRETK
                     GNQIQAAELLGLNRNTLRKKILDLDIPVKRARAS"
     gene            2858..3253
                     /locus_tag="PLS35_19210"
     CDS             2858..3253
                     /locus_tag="PLS35_19210"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNI20858.1"
                     /translation="MSPSNAPGPASVPTTEPHLTRRHKYAHASHPRVANHQRREAMTK
                     EELIAQMANSAGITKVAATVALEAFTGAVTTSLKKGKRVTLVNFGTFTISKRKARMGR
                     NPRTGEALKIPAARIPKFSAGKELKAAVK"
     gene            3385..3459
                     /locus_tag="PLS35_19215"
     tRNA            3385..3459
                     /locus_tag="PLS35_19215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3417..3419,aa:Val,seq:gac)
BASE COUNT          733 a         1039 c          945 g          745 t
ORIGIN      
        1 ccgtgcaggc cgatcgtcgc aatatggtgt tgtgcgcacg agttatgaca gccgctgatt
       61 ttgacgctga cgtcgcgcag atcttccggc acctggccgg caggaaagac ctccgcgagc
      121 gctcgggcca tcccctttga cgaggtgatc cccaatccgc acgtatccgt acccgggcag
      181 gcaatgatgt cctcgacgag ctcagcgcct ggatcgccca ggctatgcgc gacgagttcc
      241 tcatgaaacg cgctcaaacg gtcctcctga atccatcgga tgatcacgtt ttgattgatg
      301 gtggtccgga tgttgccatt ggaatagcgt tcggccaaat cggcaaccac ccacatctgt
      361 tcgcccgtca aatcgcccat cggtaacttg atggcagccg cagcaaatcc cgcctgccgt
      421 tgaggcacca cattggtccg cttccaggcc tcaaacgctg attcggcgcc attccgcgat
      481 gcggcgccat tgccgtttcc attcccattt ccgttggtgc tgttcggtgc tttcgtcggc
      541 atcaacaacg gcggcgtatc ggcatactcg agcagtttga tcggttcatg cgtcggcacc
      601 gcatatccca tggccgcata ggccgcctcc cagcgacgtt tgaattcgtc gaatccgagt
      661 ttctcgatga cgaacttcat gcgagccttg ttccgattct tgcggtttcc taaggtatcg
      721 aacactttga tcaccgcttc gatggtcggc agcaattcgt ccatcggcgt aaattctcgc
      781 agcacttgcg ccatgcgcgg tgtcgatccc aggccaccac cggccaccat ccggaagcca
      841 atggttccat ccgcccgttt cgccgcgagc aggccgatgt cgtgaatggg tgtgagggcg
      901 caatcctgcc ggcagcccga cagcgcgatc ttgaatttcc gcgggagact ctggttcagc
      961 ggattacgca gcagatggta ggcaactgtt ttcgcgtagg gcgtcacatc gaacacttcg
     1021 ccctggcaca ctccggccag atggcacgcc gtcacattac gcaccgtgtt ggcgcaggct
     1081 tcacgtgtgg tcaatcccac tgccgccaac tggcgcatca tctcaggcac gtgcttaagc
     1141 tccacgaaat gcagctgaat gtcctgacgc gtcgtgacat ggcccacgcc ggtggcaaac
     1201 tggtcggcca attcagccac gcgtcgcaat tgattggccg tcaaaccgcc gaatggaatt
     1261 ttgatccgga acatctgcac gccgggctgc cgctgaccat agatgccgta ctgcaagcgg
     1321 aagggcttga agatgtcggt cgagagatcg ccggccagca cacgttgagc ttcggcctcg
     1381 aatgtctcga tttcttcgac aatatgggga ggaatgggct cagtcttcac agcggggcga
     1441 agagcctctg tatgactact tatcataacg acctcgtcgg ttcggaagta atgtgcaggc
     1501 gcatcatgcg ggagttcaaa tgtgatacat caatgtatgt tgctcgtcga gggcccgttg
     1561 gcgctttgcc aactcctcga ccgtcacttc ggataacacg ctgagttccg cccgtttcac
     1621 acgatcccaa atatgcgcaa ggagcgcctc ttgcttggaa gctcgtgacg aggtctgttt
     1681 cggagcggcg tctccgttcg tcgacagaag cgggccttct aaggcatcga gaatgtccgc
     1741 cacagagagc tccgagggtt ttcgactcaa cacatacccg ccttgcgctc cgcgctgact
     1801 cgtcaccact cccgccttct tcatcgcatg cagcacttgc tcgaggaacc gggccggaat
     1861 cgcctgccgc ttggcgatcg actttgcgca caccggatgc tcggcatggt gaagcgcaag
     1921 atctacggca gcgatgatcc cataggtggc tctaaggcta accttcattt gcgactattc
     1981 cgatggggat tcatgaatgt ctatacattg agccccgctt tcctgtcaag cccgcgggcg
     2041 caggcctggt tgcggatatc gcggatgcgc tggcggcgat cacgttggcc gacaattgct
     2101 cgccatctct cggaagagat gaatttgctg cgtcgaaagt ttctaatttg cattgcgaat
     2161 tgatgccacc aacaatctct tctaccagtg ctcactttct ccactttgat cgatcaggct
     2221 gcatcaatac cggtgcagat ttcctcattg acatcatctg gggcctgagc aataatcccg
     2281 ccctcttctt cctcggtgcc tctcatgacg ctgcgttcat caacatttaa tattctattg
     2341 ctcagcacag acagcgagat ccagtcacac tacaaggacc ttttcggtga gcaggcgatt
     2401 accgttggcc gtgaaggccc atcactgcag aaggacctgg caaagcgcga ctacgatgcg
     2461 gtcatcatcg aatcgaagtt ggctgccgcg gcggacttga gcaaattgct gaccggaatc
     2521 gatccttcac gaaccctgct cctcgtggga tcgcgtaccg tgctgaaacg caccgccgcc
     2581 gtgcttcagg cgtccaaggc ctcgaaggcc gaagcgcctc cgtcgaatgg caaaggacaa
     2641 gctttgtgtt tggattctta tctcgaacac aaggtcgggg atttcgtcaa aggcatgcgg
     2701 aatggctcag ggaaaaatct ccacccgatg ctgatcgccg cggtcgaacg cccgttgatt
     2761 cttctgacct tgcgagagac caagggcaac cagatccaag ccgcggagct ccttgggctg
     2821 aatcgcaata cgttgagaaa aaagattctc gatctggata tccccgtcaa acgcgccagg
     2881 gccagctagc gtaccgacga ctgagccgca cctcacaagg cgtcataagt acgcccatgc
     2941 atcacatcca cgggtcgcca accaccaacg gagggaagca atgaccaagg aagaattgat
     3001 tgcacaaatg gcgaacagtg ccggtatcac caaggtcgcc gcaacagtcg cgctcgaagc
     3061 cttcaccggc gcagtcacca cgtcgctcaa gaagggaaag cgggtgacgt tggtcaattt
     3121 cggcaccttt acgatttcaa agcgcaaggc caggatggga cgcaatccgc ggaccggcga
     3181 agccttgaaa attcctgccg cccgcattcc gaagttctcg gccggcaagg aactcaaggc
     3241 ggccgtcaag taggagtcgg cagactcctg gacagcccca cgggacaaga cgcgattgcg
     3301 tggctcgggc acagagaaaa gcctgtcttg ccaccgcgtt tccttgacac ctttggtaag
     3361 cctgtattag catcgccgat cgataggcgc gtagctcagg gggagagcgc taccttgaca
     3421 cggtagaggt cgacggttca agaccgtccg cgcctaccat tt
//
LOCUS       DAOQEW010000313         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_12907_length_3450_cov_8.180265, whole genome shotgun sequence.
ACCESSION   DAOQEW010000313 DAOQEW010000000
VERSION     DAOQEW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12907_length_3450_cov_8.180265"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..815)
                     /locus_tag="PLS35_19220"
     CDS             complement(273..815)
                     /locus_tag="PLS35_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20859.1"
                     /translation="MHITLDGEQLQVPEDTSMMNALATLSDKAHAKHHIVTSLTIGGK
                     TISDRDLTPPFLNQPVRDAGAIQAISQSLHTIILEAKQAIERFAAELRSDGQVLLAPL
                     RSGTAQVGTIDAWLGRLADYTEMLEAGQAQGVEGLSSASLLPWIQELLGARTTADAIR
                     VADLLEYELLPRLADARLAA"
     gene            complement(858..2534)
                     /locus_tag="PLS35_19225"
     CDS             complement(858..2534)
                     /locus_tag="PLS35_19225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNI20860.1"
                     /translation="MNKRVLLINITRMGDLVQMGTLLQRLQHEWPGAEVDLVVDQRFA
                     PVAKLLPHLRHIVEYDFHRLVDESRAQTKDVVTLYHDMTRWAAPLVEARYDRVVNLTF
                     NRRSGLLTSYVGAKEIRGIAAPKDGDVTIHNPWMAYLTDVHHERRFNHFNLVDIYALG
                     GSGSGPFAPLSLTIPPDTAEWARNFLATQGGQVRSWTAVQVGASDPMKAWRPELFGQT
                     LAALSRQTTAGYVFIGTEEERKAIHLAQTTYRKSGGTGPLCDAVGKTSLPQLAAVLSQ
                     CRLLLTNDTGPMHLAVGVGTPVIDLSVGHVDFRETGPYGPGHWMVQPDLGCAPCGFDQ
                     VCFHHACKDRIVPDQVAALCLHVLAGVPFPRSISGVRIYRSCADEDGLGNTELQAGRE
                     DLLVDWYGRFWRRFWFEEFTGRPSRVPCDHEPSPDCSDALAVLEHLKPLAKKLVSNAE
                     ELVRLTTKRPLPVSALQQMQRLETAERQQIVALSMQSPAAASLAVAMVRDTHNDDGHE
                     LTGLAQSRLATYRRWHKRLQAVSNSFRSFTAAQGSVRRGKTRQLPMARSA"
     gene            complement(2531..>3450)
                     /locus_tag="PLS35_19230"
     CDS             complement(2531..>3450)
                     /locus_tag="PLS35_19230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNI20861.1"
                     /translation="QQLAPGYEFWFVFQQGACLDAFRQAGARQVNYLPMAADAELHCP
                     MSLSEEDRQRFGADVSFVGAGYANRRRLFPALLRQPWSFKLWGNEWDGADDLRSVLQL
                     DGARIDTATCMKVFNATAINLNVHSTTGAGLDPQADFVNPRTFELAACGAFQLVDHRS
                     QLRQFFSEQEMVSFQDFGDVPGLVQQWLNDHAARQAMANAARAHVLSEHTYVHRMRDL
                     LGHIGLSQPDRVGSVLRGERQQESLVKRCADDRPLEALIQDCPGGQRIELKDLAARIR
                     SKGPTATLKREELMVLMLDEYRSETRDLL"
BASE COUNT          723 a         1036 c         1001 g          690 t
ORIGIN      
        1 cagtgacgca acgttggtgt tgactactaa tgccatgacg tacttcctcc tgaagcttcc
       61 gcactactct cggaatcgcc agcgtccgtg ccgacgaggt ggtaaccagg tccggtggag
      121 atgtggccac atccccgctg ccccttatta ccgtgttgct gtgcccctta tcggtaggct
      181 ctcaggaaga cttaagtcgt gaaggatgcg cagaaaatgc ctgtaacgag gagcagcgcg
      241 gggcggtgtg ccctattgag caacaatgga tattaagcag ccaaacgggc atcggcgagc
      301 cggggtaata attcgtattc gagcaggtct gccacgcgga tcgcgtcggc cgtggttcgt
      361 gcgcccaaga gttcctggat ccatgggagc aacgaggcag acgacaaccc ttcgacaccc
      421 tgggcctgtc cggcttccaa catttcggtg taatcggcca gtcggccaag ccaggcatca
      481 atggttccca cctgggcggt tcctgaacgc aagggggcca gcaacacctg cccgtcggat
      541 cgcagctcgg cggcaaatcg ctcgatggcc tgcttcgcct cgagaatgat ggtgtgcagt
      601 gactgggaga tggcttgtat ggcccccgca tcacgaaccg gttgattgag aaacggcgga
      661 gtgagatcgc gatcgctgat cgtctttccc ccaatggtca atgacgtcac gatatgatgt
      721 ttggcatgcg ccttatcgct cagcgtcgca agcgcgttca tcatcgacgt gtcttccggt
      781 acctgtaact gctcgccatc aagagtgata tgcatgtcag gctcctgtaa aagaattcgt
      841 gctgtcttct tctctcgtta tgcggaacgc gccatgggga gctgtcgggt ctttccccga
      901 cgcactgatc cctgcgctgc ggtgaaggaa cggaaactat tcgacaccgc ctgcaatcgc
      961 ttgtgccacc gccgataggt agcgagtcgg gattgcgcca gtccggtcag ttcgtgcccg
     1021 tcatcattgt gggtatcacg taccatggcc acggccaggg aagcggcggc cggtgattgc
     1081 atgctgagcg caacgatctg ttgccgctcc gcagtttcaa gacgctgcat ctgttgcaag
     1141 gcgctaaccg gcaacggacg tttggtcgtg agacgaacca gctcttctgc gttcgacacc
     1201 agcttcttgg ccagaggctt caggtgctcc aacacagcga gagcgtcgga gcaatcgggt
     1261 gacggttcgt gatcgcaagg aacccggctg ggacggccgg tgaattcttc aaaccagaaa
     1321 cggcgccaaa agcgaccata ccaatcgacc agcaggtctt cccgtccggc ttgcagttcg
     1381 gtgtttccca ggccatcttc atccgcacag gagcggtaga tcctcacgcc tgagatcgac
     1441 cggggaaatg ggactcccgc gagtacatgc agacacaagg cagcgacctg gtccggcacg
     1501 atgcgatctt tgcaggcatg atgaaagcag acctggtcga atccacaggg agcgcatccc
     1561 agatccggct gcaccatcca atgaccgggc ccatagggtc cggtttcccg aaaatcgaca
     1621 tgcccgaccg acaggtcaat gaccggtgtg cccactccca ctgcgagatg catggggccg
     1681 gtgtcattgg tcagcaggag tcggcactgg gagagcacgg cggcaagttg cggtaacgac
     1741 gtctttccca ccgcgtcgca gagcggaccg gttccgccac ttttccggta tgtcgtttga
     1801 gcgaggtgaa tcgctttccg ttcttcttct gtgccgataa atacataccc ggctgtggtt
     1861 tgccggctga gagccgccaa ggtttgtccg aacagttcag gacgccaggc cttcatcgga
     1921 tcgctggccc cgacctgcac cgctgtccag gaccgtacct gtccgccctg agttgcgagg
     1981 aagttgcgcg cccattcagc cgtatccggc ggaatcgtca gtgagagcgg cgcaaacggt
     2041 ccactgccac tgccgcccaa ggcatagata tcgacgaggt tgaaatggtt gaagcgacgc
     2101 tcatgatgca cgtcggtcag ataggccatc caggggttgt gaatggtcac atctccgtct
     2161 ttcggagcgg cgataccgcg aatttccttc gcgcccacat aggacgtcag cagtccgctt
     2221 cgccgattga aggtcagatt gacgacccgg tcatagcggg cctcaaccaa cggtgccgcc
     2281 caccgcgtca tatcgtgata gagggtcacc acatctttgg tttgagcgcg actctcatcc
     2341 accagtcgat gaaaatcgta ctcgacgatg tgtcgaaggt gcggcagcag cttcgccaca
     2401 ggagcaaacc gttgatccac gacgagatcg acctctgccc ctggccattc gtgctggagc
     2461 cgctgaagca gcgtgcccat ttgcacgaga tcgcccatcc gggtgatgtt gatcagaagg
     2521 acgcgcttgt tcataagagg tcgcgggtct cgctgcggta ttcatccaac atgaggacca
     2581 tcagttcctc acgcttgagg gtggcggtcg gacctttgct acgaatacgg gctgccaggt
     2641 ccttcagctc gattcgttgc ccgccgggac agtcttggat gagcgcctcc aacggacgat
     2701 catcggcgca gcgcttcacg agcgattcct gctggcgctc gccacggagg acggacccga
     2761 cccggtccgg ctgagacaag ccaatgtgtc caagcaaatc ccgcatccga tgcacatagg
     2821 tatgttcgct cagcacatgg gctcgagccg catttgccat ggcctggcgg gcagcgtgat
     2881 cattcagcca ctgctgcacc aatccgggaa cgtcaccaaa atcctggaac gacaccatct
     2941 cctgctccga gaagaactgg cgtagctggg agcgatgatc gaccagttga aacgcgccgc
     3001 aggcggccaa ttcaaatgtg cgtggattga cgaagtcagc ttggggatcc aggcctgcgc
     3061 ctgtggtgga atgcacattc aaattgatcg ccgtcgcatt gaacaccttc atgcacgtcg
     3121 cggtatcgat cctggccccg tccagctgaa gaacagaacg caggtcgtct gccccatccc
     3181 attcattccc ccacagtttg aacgaccagg gctggcgaag cagcgccgga aaaagacgcc
     3241 gtcgattggc atagcccgcg ccgacaaacg agacatcagc cccaaaccgc tgccggtctt
     3301 cctcggataa cgacatcgga caatgcaact ccgcatcggc ggccatcgga agataattca
     3361 cctggcgcgc accggcctga cggaatgcgt cgagacatgc cccctgttga aagacaaacc
     3421 agaactcgta tcccggcgcc aactgctgcc
//
LOCUS       DAOQEW010000314         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_13159_length_3410_cov_7.220268, whole genome shotgun sequence.
ACCESSION   DAOQEW010000314 DAOQEW010000000
VERSION     DAOQEW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13159_length_3410_cov_7.220268"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLS35_19235"
     CDS             complement(<1..794)
                     /locus_tag="PLS35_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNI20862.1"
                     /translation="MAKKMQRQIAVVGLGYVGLPIAVAFGKSAPVIGFDINKTKIDEL
                     RKGVDRTGEVSPQDLKSSRVRYTCEPSDLKTADFIIVAVPTPINDALQPDLTALKKAS
                     ELIGANLSPGAIVVYESTVYPGATEEDCLPILEKASGMKSGIDFKIGYSPERINPGDK
                     EHTLERIIKVVSAQDEESLEIVAQTYGLVVKAGIHRASSIKVAEAAKVIENTQRDLNI
                     ALMNELALIFHRLGIDTRSVLEAAGTKWNFLKFNPGLVGGHCIGVDP"
     gene            1349..2290
                     /locus_tag="PLS35_19240"
     CDS             1349..2290
                     /locus_tag="PLS35_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20863.1"
                     /translation="MARLATTHILTTNKRTIMDGNATLHASKRLATIVAVLTLTFGFT
                     APQTQAAGVWTNEPAGAAVLLDCGFDTPTCAGQLFDPYGTAGNTLTVQSDSKAPLSPS
                     NVVRSTMTYPDRTGGTELHYVHPTLLKEIFVGLWWRSNPEFSGNGVGNNKTFFIRGRS
                     QQNGVFVWQKPQGTSTSRLYWTSQLPYNLNQCGGADIDQCFANVNDVTLVPGTWYKIE
                     AYFKASSCPTCRDGVLRWWINGALAGNYLNYAYGPDVSEWLWSETWDGFTAGSGFTSS
                     PAHYIDHLHISAPNGGAGTDNPPGPPAAPTMRTVTTP"
     gene            complement(2470..2940)
                     /locus_tag="PLS35_19245"
     CDS             complement(2470..2940)
                     /locus_tag="PLS35_19245"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNI20864.1"
                     /translation="MVRILTVVDQFTRECLCLLADQSLTGGKVAQALELVVGQRGAPR
                     SITVDNGSEFASRVMDAWAYRHGIQLDFIRPGKPVENGFIESFNGRLRDECLNVEVFF
                     TLDDVRETLARWQEDYNRTRPHSALQDQAPACFAADWSAITMAQAAPELLETLT"
     gene            complement(2925..>3410)
                     /locus_tag="PLS35_19250"
     CDS             complement(2925..>3410)
                     /locus_tag="PLS35_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNI20865.1"
                     /translation="HQAEAAGRGSLARSAYVAGDCPKKSVRPRDRRALARWAQVTYQV
                     SERRVARLLPIARASLQYRSRRDPQEALRMRLRELAAARVRFGYRRLTVLLRRDGWRV
                     NAKRVYRLYRAEGLTVRTKHRTKAAGRARVPQPGATAPNKRWSMDFMSERVADGRWSE
                     F"
BASE COUNT          763 a         1003 c          868 g          776 t
ORIGIN      
        1 gggtctacgc ctatacaatg tccaccgacc aggcccggat tgaatttcaa aaagttccac
       61 tttgttccgg ccgcctccag aactgatctc gtatcgattc ctaagcgatg aaaaatcaaa
      121 gccagttcgt tcatgagggc gatattcaag tctcgctgcg tattttcgat caccttcgct
      181 gcttccgcca ccttgatgct ggacgctcga tgaattccgg ccttgaccac gagcccgtat
      241 gtttgtgcga caatttccag tgactcctca tcctgcgcag aaaccacctt gatgatgcgc
      301 tccaaagtat gttccttgtc gccgggatta atgcgctcag gcgaataccc gatcttgaag
      361 tcgatgccgc ttttcatccc ggaggctttt tcgagaatcg gcagacagtc ttcttcggtg
      421 gcgccgggat acaccgtgga ctcatagacg acgatggcgc cgggggatag attcgcacca
      481 atgagttcag aagctttctt taaggcggtg agatccggct gaagcgcatc attgattgga
      541 gtcggcaccg cgacgataat aaaatcggcc gtcttcagat cgctcggctc acacgtgtac
      601 cggactcgac tggacttcaa gtcctgtggc gagacctctc ctgtccgatc gacacctttg
      661 cgtaactcat cgatcttggt cttattgata tcgaacccga ttaccggcgc agatttacca
      721 aacgccaccg ctatcggcag tccgacgtat cccaatccca ctaccgcaat ctgccgctgc
      781 atcttcttgg ccatgtcact ccccttgtca gtcacacccg caccacccgg tcaatgttga
      841 aaaaagaatg gcagaatatc gccaatccgt caacgatttc ccaggactat catatccaga
      901 tttctttcgg tttcccaaac actatgcgtg gcgctacatt ctcgactaga agaagaggaa
      961 ccgctcacat cacggaatca tcctcacctg tactcaggtg aggcattcca gttcactgga
     1021 gttgcatcct gccacatcgg ctgcttctga ttgccagacg ccacgtgttc ccgaaaggaa
     1081 cgattttggc cgaggctgtc ctcacataca cacactaagg gttgtgggtg aattgctttc
     1141 ttcattgaga tgactcactc gcgcccccaa agaatcatga aaccatttgc aagcttaggg
     1201 aggctattgt atggtaagac cgttttggac gctgcaggca ttgatcttgc tcatactcac
     1261 gaagggcggg caattcttca ggacgggaat tagctccgta gtctcagtta ccctgctggt
     1321 tccgctctag tatcgataga ccggtcgcat ggccagatta gcaaccaccc acattctcac
     1381 tacgaacaag aggacaatca tggacggcaa cgcaacactt cacgcctcca agaggttggc
     1441 tactattgtg gccgtgttaa ccctaacttt cgggttcacc gccccacaga cccaggctgc
     1501 cggcgtttgg acgaatgaac cggcgggtgc ggcggttttg ctcgattgtg gcttcgatac
     1561 ccccacatgt gctggacaat tgtttgatcc ctacggtacg gctggaaaca ctttaactgt
     1621 gcagtcagac agcaaggccc ccctcagccc ttccaatgtt gttcgctcca cgatgaccta
     1681 tccggaccgc acagggggga ctgaattaca ctatgttcat ccaacccttt tgaaagaaat
     1741 atttgtcgga ttgtggtggc gctcaaatcc cgagtttagt ggaaacggtg taggcaacaa
     1801 taagaccttt tttattagag gacgatctca acagaatggc gtatttgtct ggcaaaagcc
     1861 tcaaggaacg tccacaagtc gtctgtattg gactagccaa cttccctata acctaaatca
     1921 atgcggaggg gctgacatcg atcagtgctt tgcaaatgta aatgacgtaa cgttggtacc
     1981 cggcacctgg tacaagatag aagcctattt taaagcaagc tcctgcccca cctgccgcga
     2041 tggagtgctt cgatggtgga ttaatggcgc actggcgggc aattatttga attatgcata
     2101 tggtccagac gtaagcgaat ggctctggtc agaaacttgg gatggattta cagcaggatc
     2161 aggttttaca agtagtccag ctcactacat cgatcattta cacattagtg ctccaaatgg
     2221 aggcgccggc acagacaacc ctcctggccc tccggctgct cctacgatga gaactgtcac
     2281 aactccatga tagatgcaca gacaatgaat cgatttcgaa ccagcttggc ccctcctctg
     2341 gggccaaggc agtcctgacc gaagcggtgt ttgacccgcc ccgcgaatgt agaccaaccg
     2401 agacctgagg acttggcctg cgcgtgatcg gtttgagccg ccagagcggc catgcgaagc
     2461 tatcatagct cacgtgagcg tctccagtaa ttcgggagcg gcctgagcca tcgtgatggc
     2521 ggaccagtcc gctgcaaagc aggctggagc ctgatcttgc agcgcgctgt gtggtcgagt
     2581 gcggttgtaa tcctcctgcc atcgcgccaa cgtctcgcgt acatcgtcga gcgtgaagaa
     2641 cacttcgacg ttcaggcact catctcgaag acgcccgttg aaactctcga tgaagccatt
     2701 ctcaacgggc ttgccgggtc gaataaagtc caactggatg ccgtggcgat aggcccaggc
     2761 atccatcaca cgactggcaa actcgctgcc gttgtcgacg gtgatcgagc gtggggcgcc
     2821 acgctgcccc acgaccagtt ctaacgcttg tgccaccttc ccgcccgtga gcgactgatc
     2881 ggccagcagg cacaggcatt cacgggtgaa ctgatcgacg acggtcagaa ttcggaccat
     2941 cgaccatcgg cgacccgttc gctcatgaag tccatgctcc accgcttgtt gggggccgtg
     3001 gcccctggtt gcggcacacg ggctcggccc gccgccttcg tgcggtgctt cgtgcgtacc
     3061 gtcagccctt ctgccctata gagtcgatag acgcgcttgg cattcacgcg ccacccatcc
     3121 cgtctcagca acaccgtcag tcgccgatat ccgaatcgga cccgtgccgc ggccaactcg
     3181 cgcaaccgca tccgcaatgc ctcttgagga tcgcgccgac tccgatactg caaagaggcg
     3241 cgggcgatcg gcaacagcct cgcgacccgg cgctcgctga cctgataggt cacctgcgcc
     3301 cagcgggcca gtgcccgccg gtcccgaggc cttacagatt ttttcggaca atctcctgca
     3361 acatatgccg atcgagcgag agatccgcga ccagccgctt cagcttggtg
//
LOCUS       DAOQEW010000315         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_13695_length_3335_cov_10.306402, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000315 DAOQEW010000000
VERSION     DAOQEW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13695_length_3335_cov_10.306402"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..650)
                     /locus_tag="PLS35_19255"
     CDS             complement(114..650)
                     /locus_tag="PLS35_19255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNI20866.1"
                     /translation="MNVPNSLTMLRILLIPVYVGLLNYEQFDYALAVLFIAGLTDALD
                     GIIARVADQRTRLGEVLDPLADKLMLTTGFITLSVMHLVPLWLTILVASRDLMLMLGA
                     AVAHFTHTQVDISPTVLGKGTTLIQLATLVAVVFFASRRLDLSTLDPLLYLMGGVTLT
                     SGLHYLSRGYVRITSSQA"
     gene            827..1147
                     /locus_tag="PLS35_19260"
     CDS             827..1147
                     /locus_tag="PLS35_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20867.1"
                     /translation="MSGLPLLFKKEGLIERHQVEGIDPSDRYFNRAVLVNRVAAGYTG
                     KMTYEAYAVEGTPHSTTSAAVKAVVDKLQSVGFTRMRTRLNFKGNRYLAEKETWTDYP
                     DLSA"
     gene            complement(1144..2268)
                     /locus_tag="PLS35_19265"
     CDS             complement(1144..2268)
                     /locus_tag="PLS35_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNI20868.1"
                     /translation="MNDPIQSALQAAVDDGTFPGAVLAVRLRGEIVFESAVGRLSRQG
                     SEEAVTDHTCYDLASLTKVLATTTALALLIQRGRLKVDDRIDGILNELRDSAIGAATV
                     RQLLTHSSGLPGWRPLYERIAAIEATQPGFMGSPAACEAVTGYIAKEELIYERGSRSL
                     YSDLGFMLLGFAVERLSGEPLDQFCSGQIYEPLGARPLAYARRGPLSALSIHHDAECI
                     IAPTEEDPWRKRMLCGEVHDENAYALGGVAGHAGLFGTARAVLAVSQAWMDGWHKKRG
                     LLEHEIVRTFTTNRQGVPNSSWALGWDTPSVRSSSGTCFSLESFGHLGFTGTSLWIDP
                     VKELEVVLLSNRVHPTRRNEQIRVFRPHIHDVICRELLRN"
     gene            complement(2387..2899)
                     /locus_tag="PLS35_19270"
     CDS             complement(2387..2899)
                     /locus_tag="PLS35_19270"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNI20869.1"
                     /translation="MKITPIDIQQMVFQVKFRGFDRDEVNRFLEELALTVENLNRENS
                     LLREKLTTTEQQVADLRRTEATLSNTLVSAQTLAEDVKRSAQREADLIVKEAELKASE
                     IIRQARGSLTEMQRGVADLQKQRLMMVERFRSTLRSFERMLEVEESDAYQAEGASVEG
                     KLAGESSPAR"
     gene            complement(2921..3217)
                     /locus_tag="PLS35_19275"
     CDS             complement(2921..3217)
                     /locus_tag="PLS35_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNI20870.1"
                     /translation="MFVVSNVLQGTATVLDTVLWLYMWVIIARALISWVNPDPWNPIV
                     QFLERATEPVLTPIRRLIGWRMGMDLSPMIAILILVFLQYAVVQSLRDIAVRMH"
     gene            complement(3217..>3335)
                     /locus_tag="PLS35_19280"
     CDS             complement(3217..>3335)
                     /locus_tag="PLS35_19280"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNI20871.1"
                     /translation="SPGGTTIAGLHQLEVGGMRGCLMAAVEAATKRSQELGR"
BASE COUNT          766 a         1015 c          921 g          633 t
ORIGIN      
        1 tgcagcggaa aactatgatc gaagtctata agacgaccta aaaactgtca aacaaatgca
       61 ggaaaaacga agcccggtaa aaggccatag gagcggcgag cggtgaaggc acgctatgcc
      121 tggctggagg taatgcgtac gtatccgcgg gagaggtagt gcaggccgga ggtcagcgtc
      181 acaccgccca tcaggtacaa aagcggatcg agcgtggaaa gatcaagccg ccgcgaggca
      241 aagaaaacga ccgccaccaa cgtcgccagc tggatcaatg tcgtgccttt ccccagcacc
      301 gtcggcgaga tatcgacctg cgtatgagtg aagtgggcga ccgccgcccc caacatcagc
      361 atgaggtcgc ggctggcgac gagaatcgtc agccagagcg gcacgagatg catgacagag
      421 agggtgatga atcccgtggt cagcatcagt ttgtccgcca agggatcgag gacttccccg
      481 agcctcgttc gctgatctgc cacccgggcg atgattccgt cgagcgcatc ggtcaacccg
      541 gcgatgaaca ggaccgccaa tgcatagtcg aattgctcgt agttcagcag cccgacatac
      601 acgggaatca acagaatgcg taacatcgtc agactgttgg gaacgttcat attcatcaca
      661 agaaactgac gggacacacc atttcggttg ccggatcacg gcacaggaat cgcgcatcat
      721 agaaacccct gcggaacctt gtcaacgacg gttgcactcg acaaggcagg tccctataat
      781 cgggacccgt aagtcgcggc ctacagatga tcgacggagg tttagtatga gtggactccc
      841 cctcctcttt aagaaagagg gattaattga gcggcatcag gtcgagggga tcgatccaag
      901 cgatcgctat tttaaccggg ctgtcctggt gaaccgggtc gcggccggat acacgggcaa
      961 aatgacctac gaagcctacg ccgtggaggg taccccccat tcaacgacca gtgccgccgt
     1021 caaagccgtc gtcgacaagc tgcagagcgt gggtttcact cggatgcgca cacgcctgaa
     1081 cttcaaaggc aatcgctatc tcgccgaaaa agaaacctgg actgactacc ccgatttgtc
     1141 cgcttaattt ctcagtaatt cccggcaaat aacatcgtgg atgtgcgggc ggaagacccg
     1201 gatctgttca ttccgcctgg tagggtgaac ccgattggag aggagcacca cttccagctc
     1261 cttgaccgga tcgatccaca atgacgtgcc cgtgaacccg agatggccga acgactcgag
     1321 agagaaacac gttcctgacg acgaccgcac cgacggcgtg tcccacccaa gcgcccagct
     1381 ggagttcgga acaccctgcc tgtttgtcgt aaaggtcctg acgatctcgt gttccaataa
     1441 ccctcgtttt ttgtgccagc catccatcca ggcttgagaa acggccagta cggcccgggc
     1501 agtcccgaac aaccccgcgt gaccagcgac tccgccaagg gcataggcat tttcgtcatg
     1561 gacctcgccg cagagcatgc gcttccgcca gggatcctcc tccgttggag caatgatgca
     1621 ttctgcatcg tgatggatag acaaggccga taggggccca cgccgcgcat aggccaacgg
     1681 gcgagcgcca agcggctcgt agatctgccc actgcagaat tgatccagcg gttcgcctga
     1741 gagccgttcc acggcgaagc ccaacagcat aaaacccaga tcgctataca agctgcggga
     1801 gccccgctcg taaattaact cttccttcgc gatgtagccc gttaccgcct cgcacgccgc
     1861 tggactgccc atgaatcccg gctgcgtcgc ctcgatcgcg gcaatacgtt cgtacaaggg
     1921 gcgccatccc ggcagaccgg aactgtgggt gagcaactgc cgcaccgtgg cggctccgat
     1981 cgcactgtct cgcaactcat tcaagatccc gtcaatgcgg tcgtccacct tcaggcgtcc
     2041 acgctggatc aacaaggcga gcgcggtggt cgtcgcgaga actttggtca gagacgccag
     2101 gtcgtaacag gtatgatccg tgacggcttc ctccgaccct tgccgggaca gccggccgac
     2161 ggcgctttcg aagacgatct ccccgcgcaa gcgtactgcc agaacggcac ctggaaatgt
     2221 cccgtcgtcg acggcggctt gcagcgccga ctgtatcgga tcgttcatag ccatccgtac
     2281 gatctagcgt gaagctgagc tggcggggag aggccgggag cggacggtcc gctccgccgg
     2341 ttcaggattg caggcaaagg tggaattccc gatgaacgca ggagactcaa cgcgcagggc
     2401 tggattcgcc ggcgagcttc ccttcaaccg aagccccctc tgcttgatac gcgtcgctct
     2461 cttcaacttc cagcatccgc tcaaatgacc gcagggtaga acgaaagcgc tccaccatca
     2521 tgagccgctg tttttgcaga tccgccacgc cgcgttgcat ctcggtgaga ctgccgcgcg
     2581 cctgccggat tatttcactc gctttcagtt cggcctcctt cacgatcaag tcggcctctc
     2641 gttgggcgga tcgcttgacg tcctccgcca gcgtctgggc cgagactaat gtgttggaga
     2701 gcgtcgcttc ggtgcgtcgc aagtcagcca cctgctgctc ggtggtggtg agtttttcac
     2761 gaaggaggct attctctcga ttcaaatttt cgacggtgag cgccaactcc tcgagaaagc
     2821 ggttcacttc atcccggtca aaccctcgaa acttgacctg aaacaccatc tgctgaatat
     2881 cgatgggagt gattttcatg cgacacctct tgcccgacag ttaatgcatc cgcacggcaa
     2941 tatcccgcaa ggattgaacc acggcatatt gcaaaaagac aaggatcagg atcgcaatca
     3001 tcggcgaaag atccatgccc atgcgccatc ctatcagccg ccgaatcggt gtgaggaccg
     3061 gttccgtcgc gcgctccaaa aattggacaa tcggattcca cggatccgga ttcacccatg
     3121 atattaaggc acgggcgatg atgacccaca tgtagagcca caacaccgta tccaacactg
     3181 ttgccgtccc ttgcagcaca ttgctcacca caaacatcag cgtcccaact cctgtgaacg
     3241 ttttgtcgcc gcctcgacgg ctgccatcaa acatccccgc atgccgccga cttccaattg
     3301 atgcaaccct gcgatggtcg tgccacctgg ggagg
//
LOCUS       DAOQEW010000316         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_13941_length_3302_cov_6.279027, whole genome shotgun sequence.
ACCESSION   DAOQEW010000316 DAOQEW010000000
VERSION     DAOQEW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_3302_cov_6.279027"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLS35_19285"
     CDS             <1..284
                     /locus_tag="PLS35_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20872.1"
                     /translation="VTQHDGRKNVCKSMRGVIERRFGDLVFKTAIPLAAKIQESESLK
                     KTIFQLDRQSNAAREFMDLGREVLARMGLGALAEPEDMDEPVEVGAGES"
     gene            281..1411
                     /locus_tag="PLS35_19290"
     CDS             281..1411
                     /locus_tag="PLS35_19290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNI20873.1"
                     /translation="MTTKQVSKGKEEKPPLSKGKEVKSERPKSSHLADLEASFDRDAR
                     RIRPFALLGLDTQGEQPGPEPTTPNSPIPSTPPTSAVGGSASASVPVSPEATASSVAP
                     KVAPVVAEEGDQLRRLEEVVEVPLDRLVPSPDQPRSEVDPSADQELLESIRAYGVLTP
                     IQIRPVEEGKYEVVAGERRWRACALLGKTSIPALVRRKDRDRAAAEALIDNVVRKDLS
                     ALEEAKAYQALIERYGFQQSELAERLGCHKSRISKALSILKLPETILNVFFGPESHMT
                     AAHAEALLPLAHDEPRLHAIARRASKESWTRDRIRQEIDRKPRINEGAQAVRFVERGR
                     GGDRGFMLTILFHSNKPHEIPMIEEALKQASERIQEFRDKLT"
     gene            1837..3240
                     /locus_tag="PLS35_19295"
     CDS             1837..3240
                     /locus_tag="PLS35_19295"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNI20874.1"
                     /translation="MDVDLTPLSEPDEETLSPVGISAELVFPGTLTDPGSLSVTTPDH
                     LLSEHILGSIPLWEAKGRPTGAKRASYQKQIYRDVWRNGRLCKVSLVVYGSGDLGLPN
                     SIDLEFFRGFERWTKAAMSSGEPFNQLVKISGRELLEASGKGLGGASYQEMDRFFLRM
                     AGTMIGAGRDWRQEGESGGTEKRPRSNKGIVFHIFQTVVLPGQVNADGFVADKYEVEL
                     ATWYWMSLRSGNCIVIDHELFRDLHGSITKLLHQLLHNLFYLGRGTASQKYSELVRNW
                     QIKRHSALSLVKQQLDDAHRELLEKEFISRWEYVPIRTPEGKEFEIIWEAGPAWWATD
                     KKTREFREEMVGASQRELPQLGRELDPFLLIEQSDESSPEKDEEREASNARLLSIVLE
                     LSGRRKDPKVWEKWWKRAIASVPHPMIWRRIGEVRERNARGERLNMGSYLAKLVSIDA
                     TRLNLPWTTRDSSSSEK"
BASE COUNT          798 a          813 c          955 g          736 t
ORIGIN      
        1 ttgtcacgca gcatgatggg cggaaaaacg tctgcaaatc catgcggggc gtgattgaac
       61 gtcgtttcgg agaccttgta tttaagactg ccatcccatt ggccgccaag atccaagaat
      121 cggaatcgct aaagaagacg atctttcaac tagatcggca gagcaatgcc gcacgcgagt
      181 ttatggattt aggccgcgag gtcttggcgc gcatggggtt aggtgcattg gcggagcctg
      241 aagacatgga cgaaccggtc gaggttgggg caggtgaatc atgacgacca aacaagtctc
      301 caaaggcaaa gaggagaaac cccctctctc gaaaggcaaa gaggttaaat cggaacgccc
      361 aaagtcctca cacctggctg atctcgaagc ctcgtttgat cgtgatgctc gtcgtattcg
      421 cccgtttgcc cttctcggct tggatacaca aggtgagcag cccggaccag agcccacaac
      481 gcccaatagc ccaataccat cgacaccccc cacctcagct gttggtggta gcgcatctgc
      541 atccgtcccg gtgagtccag aagccacggc ttcgtctgtc gctcccaaag tggctcccgt
      601 ggttgcggaa gagggggatc aactacgtcg gctagaagag gtcgtcgaag tccccctcga
      661 tcggctggtg ccgagtcctg accaaccacg atcggaggtc gatccttcgg ctgaccagga
      721 gctattggaa tcaattcgcg cttatggagt cctcactccc atccagatcc gacctgtgga
      781 ggagggcaag tatgaagtgg tggccggcga gcggcgatgg cgggcctgtg ccttgctcgg
      841 caagacttcg attccggctc tggtacgtcg gaaggaccga gaccgcgccg ccgccgaggc
      901 gttgatcgac aacgttgtcc ggaaggacct gtcggcccta gaggaagcca aggcctatca
      961 agccctgatc gaacggtatg gttttcagca gtccgagtta gccgagcgcc tgggctgtca
     1021 taaatcacgc atttcaaagg cgctcagcat tctgaaattg ccagagacta tcttgaacgt
     1081 gttctttgga cctgaaagtc acatgacggc ggcccacgca gaggcattac tccctctggc
     1141 tcatgacgag ccacgtctcc acgcgatcgc tcgccgtgcc tcaaaagaat cgtggactcg
     1201 agatcgcatc cgacaggaaa tcgatcgcaa accaagaatt aatgaaggcg cccaagccgt
     1261 gcggttcgtg gagcgtggga ggggtggaga ccgtgggttc atgctgacca ttctctttca
     1321 ttccaacaag ccgcatgaaa tcccaatgat tgaagaagcc ttaaaacagg cgtcggagcg
     1381 catccaggag tttcgtgaca agttgaccta gtctgggatc ttcggtgaaa agtttcattc
     1441 aacttacgaa cagacgggcg gcatgctgaa caacgttggc aatggttttt ctcagtccga
     1501 aatatttcag catttttgaa tatttatagt attaccggat cgagcacgag cgatgagagg
     1561 gtacgagaag tgagttatcc acagccccat cgctacttga atgaagattt cgcgttagtt
     1621 cggtgaagct cacgctagtt gagtgaagaa aacggctcaa ttccttcgct agttgagtga
     1681 agagcgacgt aacttgaatg aagtcacgct acttcgatga acgacacagt ttttgtaaga
     1741 cattgattgt gctatgcaaa gcaagaagca agcgcctgac aaatcgtgtc tctacgttag
     1801 aatcttacga aagaattaat acataacgtt acgctgatgg acgttgattt aacaccgctt
     1861 tcggaaccgg atgaagagac tctttcccct gtcgggatct ccgctgagtt agtctttccc
     1921 ggcactctta ccgatccagg ctccttgtcc gttaccactc ccgatcatct cctcagcgag
     1981 catatcttgg gatcgattcc gctgtgggaa gccaaaggtc ggccgacggg agcgaaacga
     2041 gcctcttacc agaaacagat ctatagagat gtgtggcgca acggccgtct gtgcaaagtc
     2101 tcgttggtgg tctacggtag cggcgatttg gggcttccaa attcaatcga tctcgagttc
     2161 tttcgggggt ttgaacggtg gacgaaagcc gcgatgagca gcggcgagcc gtttaatcag
     2221 ttagtcaaga tctcagggcg cgaattgttg gaggcgagcg ggaaaggtct tggcggggct
     2281 tcgtatcagg aaatggatcg attcttccta cgcatggctg ggacgatgat tggcgccggg
     2341 cgggattggc gacaggaagg cgaatcagga gggacggaga agcgtcctcg ctcgaacaag
     2401 ggaatcgttt ttcatatctt tcaaacggtg gtgttgccgg ggcaagtcaa tgcggatggc
     2461 tttgtggcag ataagtacga ggttgagctt gccacgtggt attggatgag cctgcggtct
     2521 ggcaattgta tcgtcatcga tcatgaactc tttcgagatc ttcatggatc aatcacaaaa
     2581 ctgctgcatc aactgttgca caacctgttt taccttgggc gtggaacggc ttcacaaaag
     2641 tattccgaac tggtccggaa ttggcagatc aagcgacaca gtgccctgtc gctggtaaag
     2701 cagcaattgg atgacgctca tcgtgagttg ctcgaaaagg aatttatttc gcgctgggaa
     2761 tatgtgccga ttcgaacgcc tgaggggaag gagtttgaga ttatttggga ggcggggcca
     2821 gcctggtggg cgacggataa aaagacgcga gaattccgtg aagagatggt cggagccagt
     2881 cagcgtgaat tgccgcagct cggacgagag ctcgacccgt tcctcctcat tgagcaatcg
     2941 gatgagagca gtcccgagaa ggatgaggaa cgagaagctt cgaacgcgag gctattgagc
     3001 atcgtgctag agttgtccgg ccgacggaag gacccaaagg tgtgggagaa atggtggaag
     3061 cgcgcgattg ccagcgtgcc acatccgatg atctggagac ggatcggcga agtgcgtgag
     3121 cgcaatgcgc gcggcgagcg tctcaatatg ggcagttatt tggctaaact agtctcgatt
     3181 gatgcgacgc gattgaatct cccttggacc actcgagatt ctagttcttc agaaaagtag
     3241 catcccttca acgcacaccc tcacgcgtca tacatctcgt atgatggatg tcgcctatca
     3301 gc
//
LOCUS       DAOQEW010000317         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14055_length_3286_cov_10.840916, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000317 DAOQEW010000000
VERSION     DAOQEW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14055_length_3286_cov_10.840916"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLS35_19300"
     CDS             complement(<1..463)
                     /locus_tag="PLS35_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNI20875.1"
                     /translation="MTRWRGIIEEYRKFLPVTAQTPVITLGEGNTPLIRATRLAKKIA
                     PGVDLYLKYEGMNPTGSFKDRGMTMAISKAVEGGARAVICASTGNTSASAAAYGARAG
                     IAVYVLIPAGKIALGKLSQAMMHQATVIQVEGNFDQALTIVKELSATYPVEL"
     gene            complement(511..1824)
                     /locus_tag="PLS35_19305"
     CDS             complement(511..1824)
                     /locus_tag="PLS35_19305"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNI20876.1"
                     /translation="MKTEIGVGLIGFGTVGTGVARVLIENAELIRRRVGVPIKLVRIA
                     DLDITRDRGVAIPSGVLTTDIQQVLEDPRVDIVIELMGGYDLAKRVILDAVQRGKHVV
                     TANKALLAVHGEEIFAAASRRGIDLGFEASVGGGIPVIRALMEGLAANNIQSIYGIIN
                     GTSNYILSRMTSEGQRFEVVLDEAKRAGYAEADPTFDVAGIDSAHKLTIMVSLAYGTP
                     VNFKEIYTEGITGLTPLDIAYAKEFGFTIKLLAIAKCSDGEIEARVHPTMVPSASQIA
                     KVDGVYNAIQLVGDAVEDVVLYGRGAGSMPTGSAVVSDVMSIARDLLKDATGRVPPAS
                     YQPDYRRPLRIRPMEEITSLYYIRFMVLDRPGVLSQIAGVLGRYSISISSVLQQGRKE
                     GQTVPVVIMTHMAKERDIQHALREINPMPYISEPTTLIRVEGKDE"
     gene            complement(1821..3017)
                     /gene="alaC"
                     /locus_tag="PLS35_19310"
     CDS             complement(1821..3017)
                     /gene="alaC"
                     /locus_tag="PLS35_19310"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HNI20877.1"
                     /translation="MSISDGFYRIKRLPPYVFAQVQSLKLEARQRGEDIIDFGMGNPD
                     QPTPPHIVDKLIEASRKAKNHRYSASRGITKLRHAITGWYKRNYDVDLDPETEAIVTI
                     GSKEGLAHLALATIGPGDVVLTPTPTYPIHMYSFIIAGGEVRGIELRQDSDFFDDLLR
                     VYRQTLPRPRILVINFPHNPTTAVVDLEFFKKIVAFAKEHDVIVIHDLAYADIAFDGY
                     KAPSFLQVPEAKDVGVEFYTLSKAYNMPGWRVGFCVGNREVVGALAKLKSYLDYGIFQ
                     PIQIASTVALNGPQDCVKDVVQRYQNRRNVLVNGLNRIGWPVALPRATMFVWARIPDP
                     FRHMGSLEFSKLLLREAKVAVSPGIGFGEGGDEFVRFALVENEHRTRQALRGIRKVLN
                     LDDQEP"
BASE COUNT          729 a         1004 c          838 g          715 t
ORIGIN      
        1 ccagctccac cggataggtc gccgataatt ccttgacgat ggtcaacgcc tggtcgaaat
       61 tcccttcaac ctggatgacc gtggcctgat gcatcatggc ctgcgataac ttaccgaggg
      121 cgattttccc agctggaatc agcacataga cggcaatgcc agcccgagcg ccataggcgg
      181 cggccgatgc cgacgtgttg ccggtggaag cgcagatgac cgctcttgcc cctccttcga
      241 cagctttgga aatggccatc gtcatgccgc gatctttgaa cgatcctgtc ggattcattc
      301 cctcgtattt gaggtaaaga tccacacccg gggcaatttt tttggccagc cgtgtcgcgc
      361 ggatgagtgg cgtattgccc tctcccagcg tgatgaccgg cgtctgagcc gtcaccggca
      421 ggaacttccg atattcttcg ataatgccgc gccagcgagt cataacgatc tagcctgcca
      481 ccttctcatt gaggtcggaa aatgccccac tcattcgtct ttcccctcaa ccctgatcaa
      541 tgtcgtcggt tctgagatgt agggcatggg attgatctcg cgaagagcat gttggatatc
      601 acgctctttc gccatatgcg tcatgatgac gaccggcacg gtctgccctt ccttgcgtcc
      661 ctgctgcagc accgaggaaa tgctgatgct gtagcgaccc aatacccccg cgatctgaga
      721 caggacaccg ggccgatcca aaaccatgaa gcgaatgtaa tataacgaag tgatttcttc
      781 cattggccgg atacgcagcg gccttcgata gtccggctga taggaggctg gcggaacccg
      841 gcccgtcgca tccttgagta ggtcgcgggc gatggacatc acatcgctga cgaccgcgct
      901 tcccgttggc atcgatccgg cgccacgccc atacaagacg acgtcttcga cggcatcccc
      961 caccaactga atggcattgt acaccccatc gactttcgca atttgcgacg ccgacggcac
     1021 catagtggga tggacgcgtg cttcaatttc tccatcggaa cacttcgcaa tcgccagcag
     1081 cttgatggta aagccaaact ccttggcata ggcgatatcc agcggggtaa ggccggtgat
     1141 gccttcggta taaatctcct tgaagttcac cggagtgccg taagccaggc tcaccatgat
     1201 tgtcagctta tgggccgaat caatacccgc gacatcgaac gtggggtctg cctcggcata
     1261 accggctcgt ttggcctcat ctaacacgac ctcgaaccgc tgtccttcgc tggtcatccg
     1321 gctcagaata taattcgacg tcccattgat gatgccataa atggattgaa tattgttcgc
     1381 ggccaaacct tccatcaagg cgcggatcac agggatgcct ccccccacgc tcgcctcgaa
     1441 tccaaggtcg atcccgcgac gcgacgcggc agcgaaaatc tcttctccat gcaccgctaa
     1501 gagcgctttg ttcgccgtca caacatgctt gcctcgttgc accgcatcca gaatcacgcg
     1561 tttcgcgagg tcatacccgc ccatgagctc aatgacaata tcgacgcgcg ggtcttccag
     1621 cacttgctgg atatccgtgg tcaatacacc tgaaggaatc gccacgcccc ggtcacgggt
     1681 aatgtccaaa tccgcaatcc gcaccaactt gatgggcacg cccacccgcc gacggatcaa
     1741 ttccgcattc tcaatcagca ctcgagccac gccggtcccg acggtaccga agccgatcag
     1801 tcctactccg atctcagtct tcatggttcc tgatcatcca aattcaacac tttccgaatc
     1861 ccgcgcaacg cctgccgtgt gcggtgctcg ttttccacaa gggcaaaccg gacgaattca
     1921 tcgccgcctt cgccgaagcc gatgcccggc gataccgcaa ccttcgcttc acggagcagc
     1981 aattttgaaa actccagcga ccccatgtgc cgaaacggat ccggaatacg agcccatacg
     2041 aacatcgtcg cccgaggcaa cgccaccggc caaccgatac gattcagccc gttcaccagg
     2101 acattccggc ggttttgata ccgttgcaca acgtctttca cacagtcctg cggtccattc
     2161 aatgctaccg tactggcgat ttgaatcggc tggaaaatac cgtagtcgag atagctcttc
     2221 agcttcgcga gtgcgcctac cacttcacgg ttaccgacac aaaacccgac gcgccagccc
     2281 ggcatattgt aggccttcga aagcgtataa aactcgacgc ccacatcctt cgcctccggc
     2341 acctgcagaa aactcggcgc cttatagccg tcgaaggcga tatccgcgta ggccaaatca
     2401 tgaatcacga tcacatcgtg ctctttggca aacgcgacaa tcttcttaaa gaactcgaga
     2461 tcgaccacgg cggtcgtggg gttgtgcggg aaattgatca ccagaatacg cggacgcggc
     2521 aacgtttggc gatagacccg cagtagatcg tcgaagaaat cgctgtcctg acgcagctcg
     2581 atgccacgca cctcgccacc cgcgataatg aaactgtaca tgtggatggg ataggtcgga
     2641 gtcggcgtca ggaccacgtc gcccggcccg atggtggcca aagccaagtg tgcgagacct
     2701 tccttcgagc cgatcgtgac aatcgcttcg gtctcgggat ccaaatccac atcgtagtta
     2761 cgcttgtacc agccagtgat cgcgtgacgc agtttggtaa tgccacgcga ggctgaataa
     2821 cggtgattct tggctttcct ggacgcctcg atcagtttgt cgacaatatg gggcggcgtg
     2881 ggctgatccg gattgcccat cccgaagtcg atgatgtcct cgccccgttg acgcgcctcg
     2941 agtttcaggg attgcacctg ggcaaatacg tacggaggaa gtcgtttgat gcggtaaaaa
     3001 ccgtcgctga tcgacatgga tgtgtcagac tcctactggc ggggcctctg ctgaacgggt
     3061 tgcgtcgtga cgcctctgaa aggagcgtat tttaatagag ccctcaaaac gagtcaattt
     3121 cactatattt ctatggagtt gcggcttgcc gctcggccat gaacgtgaga aaagcgcggc
     3181 agcgccttga ccagagcctt cttgccccgc cccactgtct tctgttaata atgcctcagg
     3241 aacaacagaa ttgtctatga atttcgacca gttccgaaat ctttct
//
LOCUS       DAOQEW010000318         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14226_length_3263_cov_8.053304, whole genome shotgun sequence.
ACCESSION   DAOQEW010000318 DAOQEW010000000
VERSION     DAOQEW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_3263_cov_8.053304"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLS35_19315"
     CDS             complement(<1..866)
                     /locus_tag="PLS35_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNI20878.1"
                     /translation="MGKLLVVGSVALDTVKTPFGEVAEVLGGSATYFSTAASYFTSVD
                     LIAVVGDDFPEQHVAFLKSRKIDLAGLERRPGATFRWKGAYSHQLNEAQTLDTKLNVF
                     ETFRPKIPAQYSSPDVLFLGNIDPNLQLDVLQQVKRPALVACDTMNFWINGKRDALWN
                     VLQHIDILIINDGEARALGEDTNLVKVAKKILARGPKHLIVKRGEYGVLMFNDKQVFG
                     APAFPLDDVRDPTGAGDTFAGGFLGYLAATGNRSPEAMRQAIIFGSVMASFTVEAFSL
                     DRLRILDYKEIEA"
     gene            complement(870..1769)
                     /gene="mtnP"
                     /locus_tag="PLS35_19320"
     CDS             complement(870..1769)
                     /gene="mtnP"
                     /locus_tag="PLS35_19320"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNI20879.1"
                     /translation="MKKAKKTIQPAVGIIGGSGLYQVEGLEQVREVRLRTPFGAPSDA
                     IVVGVLGGMGVAFLSRHGRGHRINPSSINYRANIYALKSLGVTQVISISAVGSMKESI
                     EPGHVVLPDQFIDLTKRRISTFFDEGIVAHVGFSEPVCSSLADTLEEAGRSAGATLHR
                     GGTYVCMEGPQFSTKAESRLYRQWGVDVIGMTNMPEAKLAREAELCYATMALATDYDC
                     WHETEEAVTVGAILATLHRNVALAKEILKTAVPKLDPDRACACRQALATALITAPDKM
                     SASAKRRLNLLIAPYVRTRKGKR"
     gene            complement(1766..2794)
                     /gene="miaA"
                     /locus_tag="PLS35_19325"
     CDS             complement(1766..2794)
                     /gene="miaA"
                     /locus_tag="PLS35_19325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNI20880.1"
                     /translation="MIRLTESVLASRPLVVLVGPTAVGKSDVGLRLARALDTDLLTAD
                     SRQVYRGMDIATDKPAVAQQQGVPHRLIDLVDPDESFNAGQYRQLALQEIERLYGERR
                     LPLIVGGTGLYVRTLIHGLCDAPRADQAFRDSLLQKAQGQGRFFLHAELAQVDPELAA
                     RLHPHDEVKIVRALEVFHLSGRRLSDVQQEHRFAEQPFSVLMIGLNRDRAELYRRIDE
                     RVEAMFARGVVEETAALLAKGYGRETGAMKGLGYQQVAGYLSGDYDRAEALRLLKRDT
                     RHFAKRQLTWFRKEPGLQWWMLTEQDTAEDVAGRLLETIHTFVQTLNDERQPHRPAPR
                     TIEMESTS"
     gene            complement(2814..>3263)
                     /locus_tag="PLS35_19330"
     CDS             complement(2814..>3263)
                     /locus_tag="PLS35_19330"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20881.1"
                     /translation="WQGRAVTSQPLLLPEPLELPVQQAVILQRHLPELERLGLLIEPF
                     GPSSFLIRSLPIMLGHPDLSALVQDLIDDLEQWDSISSLETKVKPILASLACHAAVRA
                     GRSMALPEITQLVQDWVAEGLIMTCPHGRRVAFRLSGDELARLFDRA"
BASE COUNT          674 a         1052 c          878 g          659 t
ORIGIN      
        1 gcctcaatct ctttgtaatc taggatcctc aatcggtcaa ggctaaaggc ttctactgta
       61 aatgaggcca tcacactccc gaaaataatg gcctgccgca tggcttcggg tgaacggttt
      121 cccgtcgccg ccagatagcc caggaagcct ccggcaaagg tatcgccggc gccggtggga
      181 tcgcgcacat catccagcgg aaacgccggc gccccgaaca cctgcttgtc gttgaacatc
      241 agcacaccat attcgccgcg tttcacgatc agatgtttcg gtccccgcgc gaggatcttt
      301 ttggcgacct tcacgaggtt ggtatcctca cccaaggccc gtgcctcgcc gtcgttgatg
      361 atcagaatgt cgatgtgttg caggacgttc cagagcgcat cacgtttgcc gttgatccag
      421 aaattcatcg tgtcgcaggc gaccaacgcg ggccgcttca cctgctgcag cacgtcgagt
      481 tgcaggttcg ggtcgatgtt gcccagaaag agcacatcgg gcgagctgta ctgggccggg
      541 atcttcggcc ggaacgtctc aaagacgttc agcttggtgt cgagcgtctg cgcctcgttg
      601 agttgatgcg aataggcgcc cttccagcga aacgtcgcgc ctggccgccg ttccaatccc
      661 gccaaatcaa tttttcggct ttttaagaac gcgacatgtt gctcaggaaa gtcatcgccg
      721 accaccgcga tcagatcgac cgaggtgaaa taactggccg ccgtcgaaaa atacgtcgcg
      781 gatccgccaa gtacctcggc cacctcgccg aaaggagttt tcacggtatc cagcgccacc
      841 gaccccacta ccagcaattt acccatgcct caccgttttc ctttccgcgt ccgcacatag
      901 ggagcgatca agaggttcaa ccgtcgtttt gccgaagcag acattttgtc cggggcagta
      961 atcagtgccg tggcaagcgc ttgccgacag gcacaggccc ggtcgggatc caacttcggc
     1021 accgccgtct tcaatatttc cttagccagg gccacattcc ggtgcaaggt ggccaggatg
     1081 gccccaaccg tcactgcttc ttccgtctca tgccaacaat cgtagtcggt ggccagcgcc
     1141 atcgtggcgt aacacagttc ggcctcgcgg gcgagtttgg cttcaggcat gttggtcatc
     1201 ccgatcacat ccacgcccca ttgtcgatac aggcgggatt ccgctttggt cgaaaactgc
     1261 ggtccttcca tgcacacata ggtgccgccg cgatgaagcg tcgcgcccgc ggatcgtccc
     1321 gcctcctcta gcgtatcggc taaggaagag cacaccggct cgctgaaccc gacatgcgcc
     1381 acgatccctt cgtcaaaaaa cgtcgagatc cgccgcttcg tgagatcgat aaactggtcg
     1441 ggcaacacca catggcctgg ttcaatggat tccttcatgc tcccaacggc actgattgaa
     1501 atgacttggg tcacccctaa cgactttagt gcatagatat tggcgcgata gttgatgctg
     1561 ctggggttga tccggtgccc gcgcccatgc cgggaaagga acgccactcc catgccgccc
     1621 agcactccga ccacaatggc atccgatggt gcgccgaagg gtgtgcgcag acgtacctcg
     1681 cggacctgct ctaatccttc gacctggtac aacccgctcc cgccgataat accgacagca
     1741 ggctgaatgg tcttctttgc tttcttcatg atgtcgattc catttctatg gtacgcggcg
     1801 caggccgatg cggctggcgc tcgtcattca gtgtctgtac aaacgtgtga atggtctcca
     1861 gcagacgccc cgccacgtcc tctgcggtat cctgctcggt cagcatccac cactgcagac
     1921 caggctcctt tcgaaaccag gtcagctgac gcttggcaaa atgtctggta tcgcgtttca
     1981 gcagacggag cgcctccgcg cggtcatagt caccggacag atagcctgcg acctgctgat
     2041 accctaaccc cttcatggca cccgtctcgc ggccgtatcc cttcgccagc agcgctgcgg
     2101 tttcctcgac gacgcctcga gcaaacatcg cctcgacccg ctcatcgatc cgccggtaca
     2161 gttctgcgcg atcgcgattc aatccgatca tgagcacgga gaacggttgt tcggcaaagc
     2221 gatgttcctg ctggacatcc gagagccgcc gtccggacaa atgaaatacc tcgagagcgc
     2281 gcacgatttt cacttcatcg tgcggatgca gtcttgccgc cagttcagga tcgacttgcg
     2341 ccaattcggc atggaggaaa aatcgtccct gcccctgagc tttttgcagt aacgagtcac
     2401 gaaacgcctg atccgcccgc ggggcatcac aaagcccatg aatcagcgta cgcacgtaga
     2461 gcccggtgcc gcccacgatc aatgggagcc gccgttctcc gtacagccgt tcgatttcct
     2521 gcaacgccag ctggcgatac tgcccggcat taaacgattc atcaggatcg acgaggtcga
     2581 tgagtcgatg cggcaccccc tgctgctgcg ccacggcagg cttatccgtc gcgatatcca
     2641 tcccgcgata gacctggcgc gagtccgccg tcaagaggtc ggtgtccaac gcgcgggcca
     2701 gccgaagacc gacgtcgctt ttccccacgg cggtcggccc gaccagtacc acgagcggcc
     2761 gcgacgccag gacggattcc gtgagccgga tcatatctct ccgtgtgctt cccctaggcg
     2821 cgatcgaaca gacgcgccag ttcgtctccc gacagacgga acgccacacg ccgtccatgt
     2881 ggacaggtca tgatcaggcc ttccgcgacc cagtcctgaa ccagctgggt gatctcgggc
     2941 aatgccatcg accggcccgc gcgaaccgct gcgtgacagg ccagtgacgc cagaataggc
     3001 ttcaccttcg tttccagcga cgaaatggaa tcccactgtt cgagatcatc aatcaggtcc
     3061 tgcaccaagg cggacaaatc aggatgaccc aacatgatcg gcagactccg gatcagaaac
     3121 gatgaaggtc caaatggctc aatcagcaat cccagtcgtt ccaactccgg tagatgtcgc
     3181 tgcagaatca cggcttgctg caccggcaac tcgagcggtt ccggcaaaag cagtggttgg
     3241 gacgtcaccg cgcgcccctg cca
//
LOCUS       DAOQEW010000319         3236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14440_length_3236_cov_8.882427, whole genome shotgun sequence.
ACCESSION   DAOQEW010000319 DAOQEW010000000
VERSION     DAOQEW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14440_length_3236_cov_8.882427"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLS35_19335"
     CDS             <1..422
                     /locus_tag="PLS35_19335"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNI20882.1"
                     /translation="TMGGTNPYIEKADVELPSKAYTVTFVFPDKTEKKVEVQPDKIPY
                     GPTGLPGSILDIAMGNDIDLEHVCGGVCACSTCHVMVKKGLETCNEGTDDEFDQLDEA
                     PATTLQSRLGCQCVPNGTTDVTVEIPAINKNLAKEGH"
     gene            complement(450..1148)
                     /locus_tag="PLS35_19340"
     CDS             complement(450..1148)
                     /locus_tag="PLS35_19340"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNI20883.1"
                     /translation="MAEPTQLAEVVSLTALTPHTTELVLRPIESPLPFKPGQWISLQL
                     PIGDHPPLNRAYSLAEPPTSKGHLTLVFDHVPGGQGSGYLSSLKPGDRLPLSGPYGHF
                     VLPEPEMQHLLLIGRYSGLVPLRCMIRALAEQKTLPATTLIAHAPTASEQLYHDEFTA
                     LAARHSHFQYLPFVSETPDGHVGTLEVIKQLAGNGRRVTPMIAGIKAFARPLRSLFMD
                     LGFDRREVKLETYD"
     gene            1266..2681
                     /gene="gltX"
                     /locus_tag="PLS35_19345"
     CDS             1266..2681
                     /gene="gltX"
                     /locus_tag="PLS35_19345"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNI20884.1"
                     /translation="MSQVRVRFAPSPTGFLHIGGVRTALFNWLFARQQNGVFILRIED
                     TDQDRSTDASIQAILDGMRWVKLDWDEGPFRQTERMDLYRSHALRLFEQGHAYWCVCT
                     PEELEARRKEAEAKGGSPRYDGRCRNLGLAKPTSDAALRFKAPQDGQTVVDDLIKGRV
                     VFDNQILDDVIILRSNGFPTYNFSVVVDDALMGITHVVRGDDHLTNTPRQIPIFQALG
                     FPLPRFGHLPMILGSDKTRLSKRHGATSIMAYKEMGYLPEAMVNYLVRLGWSHGDQEL
                     FSRDELIEKFSWKNVQTSPAVFNPDKLLWLNAEYLKTNPPQQVAEALVPLLEQTDLKD
                     EVRAVSTEWLAQLVVLVKERTKTLVEMVHWVRPYFGEAVSFDEEAAKKFLTPAQAPIL
                     TKLVERFEALPVFSKHEWEEAFKKLVEEAGIKMGQLAQPVRVALTGRTASPGLFEVME
                     VLGRERTLIRLRKGIEQAARS"
     gene            2749..2822
                     /locus_tag="PLS35_19350"
     tRNA            2749..2822
                     /locus_tag="PLS35_19350"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2781..2783,aa:Gln,seq:ctg)
     gene            2876..2949
                     /locus_tag="PLS35_19355"
     tRNA            2876..2949
                     /locus_tag="PLS35_19355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2908..2910,aa:Gln,seq:ttg)
BASE COUNT          703 a          862 c          978 g          693 t
ORIGIN      
        1 gcaccatggg tggaacgaat ccctatattg aaaaagcgga cgtcgaattg ccgtcgaagg
       61 cgtataccgt cacgttcgta ttcccggaca agaccgaaaa gaaggtggaa gtgcagccgg
      121 acaagattcc ctacggtccg acgggcctac cgggcagcat tctggatatc gccatgggga
      181 acgacatcga cctggagcat gtgtgcggag gggtctgtgc ctgctccacg tgtcatgtca
      241 tggtgaaaaa gggtctggag acgtgcaatg aggggacgga tgatgagttc gatcagttag
      301 acgaggctcc cgccacgacg ctgcaatccc ggcttgggtg ccagtgcgtg ccgaatggga
      361 cgacagacgt caccgtggag attcccgcga tcaataagaa tctggcgaag gaaggtcatt
      421 gattcagcaa gacacagcct gatgaggctt caatcgtacg tttcgagttt cacttcccgt
      481 cgatcaaacc ccaggtccat aaacaggctg cgcaacgggc gggcaaaggc cttaatcccc
      541 gcgatcatgg gcgtgacgcg gcggccgttt ccagccagtt gtttgatcac ctccagagtc
      601 cccacatgcc cgtccggggt ctcgctcaca aacggcaggt actgaaaatg ggagtgcctc
      661 gctgccagcg ctgtgaattc atcatgatag agttgctcgc tggcggtggg ggcgtgcgca
      721 atcagggtgg tcgcgggcaa cgtcttctgc tctgccagtg cgcggatcat acagcgcagt
      781 ggaacaaggc ctgaataacg accgatcaac agcaaatgtt gcatttccgg ctctggcaac
      841 acgaaatgac catagggccc cgagaggggg agacgatcgc cgggcttgag tgacgaaaga
      901 tagcccgacc cttgacctcc cggaacatga tcgaacacca gtgtgagatg tcctttggag
      961 gtcgggggct ctgccaaaga ataggcccga ttcaatgggg ggtggtctcc gatcggcaat
     1021 tggagcgaga tccactggcc gggtttgaag ggcaggggag actcgatcgg acggagcacg
     1081 agttcggtgg tatggggagt caacgccgtt agcgagacga cttcagcaag ttgtgttggt
     1141 tcagccatgg actgttctta gcaaaaacag attcagaatg gaacagcccc gttctgtaca
     1201 tcgttccggt gccatgatat agataccccc ccgttaattt ctcccacaca cactacggtg
     1261 acgcgatgag tcaggtcagg gttcgatttg cgcccagtcc gaccggcttc ctccatatcg
     1321 gcggggtgcg gacggccttg ttcaactggc tgtttgcgcg gcagcagaac ggcgtgttca
     1381 ttctccgcat cgaagatacc gaccaggatc ggtcgaccga tgcatcgatc caagctattc
     1441 tcgatggcat gcgctgggtc aaactggatt gggatgaagg tccctttcgg cagaccgaac
     1501 gcatggatct ctatcgtagc catgctctgc ggctgtttga gcagggccat gcctactggt
     1561 gcgtgtgcac accggaggaa ctggaggctc gccggaaaga agcggaagcc aagggaggat
     1621 cacccagata tgacggtcgg tgccgaaacc tgggtctcgc caagccaacc agtgacgcgg
     1681 cgctccggtt caaagcgccg caggacggtc agacggttgt tgatgacctg atcaagggcc
     1741 gggtggtgtt cgacaatcag atcctggatg acgtcatcat tctcaggtcg aacggctttc
     1801 ccacctataa cttctcagta gtggtggacg acgcgttgat gggcatcaca cacgtggtgc
     1861 ggggggacga ccatctcaca aacaccccgc gccaaatccc catttttcag gccctcggat
     1921 tcccgctgcc acgcttcggc cacttaccga tgatcctggg ttcggacaaa acgcggctgt
     1981 cgaagcgtca cggcgcgacg tcgatcatgg cttacaagga aatgggctat ctgccggagg
     2041 ccatggtcaa ctatctcgtc cggcttgggt ggtcgcatgg ggatcaagag ctcttttcgc
     2101 gagacgaact gatcgagaag ttctcctgga agaacgtcca gacctctccg gctgtcttca
     2161 accctgacaa gttgctgtgg ctcaatgccg aatatcttaa gaccaatccg cctcagcagg
     2221 tggcggaagc gctggtgccg ttgctcgaac agaccgacct caaagacgag gtccgcgcgg
     2281 tctcgacgga gtggctcgcg cagctcgtgg tcttggtgaa agagcggact aagacattgg
     2341 ttgaaatggt tcattgggtg aggccttact ttggcgaggc ggtgtcgttt gatgaggagg
     2401 ccgcgaaaaa attcctgact ccggctcagg cccctatatt gactaagctg gtggaacgct
     2461 ttgaggcgct tccagtcttt tccaagcacg agtgggaaga ggcgttcaag aagttggtcg
     2521 aggaagcggg aatcaaaatg gggcagttgg cccaaccggt gcgggttgcc ttgactggcc
     2581 ggacggcgag tcccggcttg ttcgaagtga tggaagtgct cggccgggaa cgtacgttga
     2641 ttcgattgcg caaggggatc gagcaggccg ccagatcctg attggctgtt caagaatcac
     2701 gctcgatctt gacgcaatgg agaggcttat attactgtgt ggaccggttg ggggatcgtc
     2761 tagcggtagg acgtcagtct ctggatctga ctacctaggt tcgattccta gtcccccagc
     2821 caaaatcttt tccacgcctt gctcttcagc tcgatgtctc ggttccgtac cgcgctgggg
     2881 gatcgtctag cggtaggacg ccagcctttg gagctggcta cctaggttcg attcctagtc
     2941 ccccagccaa acttatctct ccacaatttc agtaacttgc gagttttcca gagcccggtt
     3001 cgattcatga gccggtggac tggtcacggt tttggtcacg gttcgagcga gactcccccg
     3061 gttgactgcc tcttggaggt gaccaggaga taaatgggag taacggagcg tggtctgaat
     3121 atccctgtgt ccaaggatct ccttcactga aactaaatcg actccagcca taacacggcg
     3181 actggccgcc gtatgcctca atgtgtgcca acaaaccccc tcaatgccga cttttt
//
LOCUS       DAOQEW010000320         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14462_length_3233_cov_7.432662, whole genome shotgun sequence.
ACCESSION   DAOQEW010000320 DAOQEW010000000
VERSION     DAOQEW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14462_length_3233_cov_7.432662"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLS35_19360"
     CDS             complement(<1..638)
                     /locus_tag="PLS35_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNI20885.1"
                     /translation="MAMVHEHDGLAFLFDLDGTLVDSVYQHVLAWREATQAAGIELPV
                     WRIHRQIGMSGGLMLHALLRETGRPVSKEEAERIQRVHAEAFVRQAPSLRILPGAQAL
                     LDALTAGRVPYAIATSGRLQSAQHTLKLLKLPEGTPVVTRDQVKRAKPDPDLFLAAAE
                     QLKVPIAKCIVVGDSVWDLLAARRASALGVGLLSGGYGREELERAGAYRTYH"
     gene            complement(644..1069)
                     /locus_tag="PLS35_19365"
     CDS             complement(644..1069)
                     /locus_tag="PLS35_19365"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNI20886.1"
                     /translation="MKRNASAKWQGDLKTGKGTVSTESGVLAGTQYSFSTRFENGKGT
                     NPEELIAAAHAGCFTMALSGQLGAANLVADQLATTATVTMEKLEAGWTVTGIHLDVKG
                     KVPKADNAAWEKATTAAKTGCPISRLLNTTITMDAKLES"
     gene            complement(1084..2094)
                     /locus_tag="PLS35_19370"
     CDS             complement(1084..2094)
                     /locus_tag="PLS35_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HNI20887.1"
                     /translation="MKAVQATAPGAAWELVERDIPRPKPDQVRVKVQACGICHSDMFV
                     KEGHWPGLQFPRIPGHEIAGLVDEVGSAVTAWKPGQRVGVGWHGGHCGQCDACRRGDF
                     ILCRSGQVCGISYDGGWAEYVVVPEQAVAALPDELSFEEAAPLLCAGITTFNSLRHSG
                     ARAGDLVAVQGIGGLGHLGIQYASKLGLKTVAVGRGKDKEPLARQLGAAVYLDAAVVN
                     PAEELQRLGGARVVLATAPDSKAIASMVDGLGANGTLLIVAAPGEPVMVNAITLIGKR
                     ASVQGWPSGTAKDSEDTLRFSAMTGVRPMIERYPLARAPEAYQQMMSGKARFRAVLTM
                     NS"
     gene            complement(2237..2608)
                     /locus_tag="PLS35_19375"
     CDS             complement(2237..2608)
                     /locus_tag="PLS35_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI20888.1"
                     /translation="MATPVMAHDSAKAKEFFEAKMAFTTGPVELERMMKNKEVNVVDV
                     RAAEDYAEGHIPGAVSLPKDQWAGLKGLRKDKVNVLYCYSQVCHLAATAAVQFAGQGY
                     PIMELEGGWRWWKDDGFDVEK"
     gene            complement(2769..>3233)
                     /locus_tag="PLS35_19380"
     CDS             complement(2769..>3233)
                     /locus_tag="PLS35_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNI20889.1"
                     /translation="FQIMFSKVLAMAGVILLATALALYGVLHPVFHVPMKGSALLFFL
                     LTALHVFTTAGFGLMAATLAKNQAQVAMMALFLVGPMLLLSGITSPFESMPRWVQAIM
                     TLSPLRYYIDITYGVMLKGAGLDLLWKPVGAMLLLGGALFGSGMWRFRRQFQ"
BASE COUNT          641 a         1075 c          871 g          646 t
ORIGIN      
        1 tcatggtagg tacggtacgc acccgcccgt tccaattcct ctcgaccata gccgcccgat
       61 agcaggccaa ctccaagggc agaggcacgg cgggctgcca ggaggtccca cacactgtct
      121 cccaccacga tacacttcgc aataggaacc ttgagctgtt cggcagcagc gagaaacaaa
      181 tcaggatccg gtttggcccg tttcacctga tcacgcgtca ccaccggcgt gccttcaggc
      241 aacttcagca atttcaaggt atgttgcgcg ctctggagcc gtccgctggt cgcgatggca
      301 tagggcacac ggcccgccgt cagcgcatcc agcagagcct gcgcgccggg caagatgcgg
      361 agtgatggcg cctgcctcac gaacgcctcc gcgtggactc gctgaatgcg ctccgcctcc
      421 tccttcgaaa cgggacgacc ggtttcgcgc aagagggcat gcaacatgag accgccgctc
      481 atgccgattt ggcgatggat cctccagacc ggcaattcga tacctgcggc ttgagtggct
      541 tcacgccagg ccagcacatg ttgatagaca ctgtcgacca acgtaccgtc caggtcgaag
      601 aggaacgcga gcccgtcatg ctcatgcacc atggccatat ctgtcagctc tccaacttgg
      661 cgtccatggt gatggtggta ttcagtaagc gggaaatggg gcagccggtt ttggccgctg
      721 tggtcgcctt ctcccatgcc gcattgtcag ccttgggcac tttccccttt acgtccaggt
      781 gaatgccggt cacggtccat cctgcctcaa gcttctccat ggtgactgtg gcggtcgtcg
      841 ccagctggtc ggccacgaga ttcgccgcgc ccaattgacc ggagagtgcc atggtaaaac
      901 aaccggcatg ggcggcagcg atcaactcct ccgggttcgt ccctttgccg ttctcgaacc
      961 tggtactgaa tgaatattgc gtcccggcca acacaccgct ctcggtcgag accgtccctt
     1021 tgccggtttt gagatctccc tgccactttg ccgacgcatt gcgtttcatg accacctccg
     1081 ttgttaagaa ttcatcgtca ggacggcgcg aaagcgtgcc ttgccgctca tcatctgctg
     1141 ataggcttcc ggtgcgcgcg ccagtggata ccgttcgatc atgggccgca cgccggtcat
     1201 cgcgctgaat cgcagcgtgt cttcggaatc cttcgccgtg ccggacggcc agccctgcac
     1261 ggaggctcgt ttaccgatca gcgtgatggc attcaccatt accggttcgc ctggcgcggc
     1321 cacgatgagc aaggttccat tcgcacccag tccgtcgacc atcgatgcga tcgctttgct
     1381 gtccggcgcg gtcgccagaa cgacccgggc gccgcccagt cgttgaagct cctcggctgg
     1441 attcaccacc gcggcatcga gatagaccgc cgcaccgagc tgacgggcca gcggctcctt
     1501 gtctttaccg cggccgaccg ccactgtctt gagtcccagc ttcgaggcat actgaatgcc
     1561 gagatgtccc agcccgccga tgccttgcac ggcgaccagg tcacccgccc gagcaccact
     1621 gtggcgcaaa ctattgaagg tggtaatgcc tgcgcagagc agtggagcgg cctcttcaaa
     1681 cgagagttca tccggcaggg cagccaccgc ttgctccggc accaccacat attccgccca
     1741 ccctccgtcg tagctgatcc cgcagacctg ccccgaacgg cagagaataa aatctccccg
     1801 gcgacaggcg tcgcactggc cacaatggcc cccgtgccag cccacaccaa cacgttgacc
     1861 aggtttccac gctgtgacag cggatcccac ctcatcgact aatccggcaa tttcatgccc
     1921 cgggatacgg gggaattgca gccccggcca atgcccttct ttcacaaaca tgtcactgtg
     1981 gcagatacca caggcctgga ccttcacccg cacctgatca ggtttcggtc tgggaatatc
     2041 tcgttccacc agctcccatg cagctcccgg agcagtggct tgtacagcct tcatgcgcgc
     2101 catcatgatg ccctttctga atacacgtat gtggcaccgg cgtggcgcaa cctgtcctta
     2161 acggaaagac tgccccacgc cctctatctt ctgcgaccac gtcctacccg ggagcgtgaa
     2221 tcgatgaacg gccgcttcac ttctcgacgt cgaagccgtc gtctttccac catcgccacc
     2281 ctccctccaa ttccatgatc ggatagccct gccccgcaaa ctgcaccgca gcggtggcgg
     2341 caagatggca gacttgcgaa taacagtaga ggacgttgac cttgtccttg cgtagacctt
     2401 tgaggcccgc ccactggtcc ttgggcaaac tgaccgcgcc gggaatatgc ccttcggcgt
     2461 aatcttcggc cgcgcgcaca tcaaccacat tcacttcttt attcttcatc atccgctcca
     2521 gttcgaccgg accggtggta aatgccattt tcgcctcgaa gaactccttg gctttggccg
     2581 agtcgtgcgc catcactggt gtcgccatgg tcatcctccc tctgaatcgt gacgtgtgta
     2641 tgctgttcgc gtcggactgg cctcccaggc tcaagcggga cgccagcctc acgggttggc
     2701 ttctaccttg taacgttaga gagtctggtg cctctatgca agcgctgatc gctcgccttt
     2761 tggatggctc attggaattg tcgtcgaaac cgccacatcc cggaaccgaa cagcgccccg
     2821 cccaacaaca gcatggcacc gaccggcttc cacaacagat ccaagccggc ccccttcaac
     2881 atcacgccgt aggtgatgtc gatgtagtag cgaagagggg agagggtcat gatcgcctgc
     2941 acccatcgag gcatcgattc aaatggcgag gtaatgcctg acaacaacag catcgggccc
     3001 accaggaaga gcgccatcat ggccacttgc gcctgattct tcgccagagt cgccgccatc
     3061 agcccgaatc cggccgtcgt gaagacatgc aaggcggtca gcagaaaaaa gagtagtgcc
     3121 gatcccttca tcggcacgtg aaacacggga tgcaacacgc cgtacagggc cagtgcggtc
     3181 gcgaggagaa tcaccccggc catggcgagc actttcgaaa acatgatctg aaa
//
LOCUS       DAOQEW010000321         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14785_length_3193_cov_10.240280, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000321 DAOQEW010000000
VERSION     DAOQEW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14785_length_3193_cov_10.240280"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..2782
                     /locus_tag="PLS35_19385"
     CDS             80..2782
                     /locus_tag="PLS35_19385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20890.1"
                     /translation="MPELDFDQSAAPSDVPPTSLGDITELDDGSILVEEPSEEVVSSA
                     DDHYANLAETLDPFNLNRIATDLLELIEKDKEARKERDKQQEEGIQRTGLGDDAPGGA
                     TFDGATKTVHPVLAEGCVDFSARAIKELFPSNGPVKTKTLGEETAALLNKAKRKRDFL
                     NFYITNRMPEYRSEKEVLLTQLPLGGSQYEKYWFDGKRVRMEFVPIDKVYLPFSANSF
                     YTSQRVTYEREVTEETVRREVASGFYRDVFSTMATEEPEESSSQAATNKVEGKTASGY
                     NEDGVRIVYEVACNYSLKEEDDPAPYIIHIDQPTNKVVAIYRNWKPSDNTKEKLDWWV
                     EDKFIPWRGAYGIGLFHLVGGMAASLTGALRALLDSAHINNAPTAIKLKGGRTSGQNV
                     NVEVTSVTEVEAPAGVDDIRKIMMPMPFNPPSTVLFQLLDWLTNQAKGVVATAEERIA
                     DAGANMPVGTALALIEQGSQVFSSIHARLHDSQRRALQIICRLIADFPDHALADLGRF
                     DLVPEDFLSTDDIEPVSDPNIFSETQRFAQMQAVMQMAAADAPDPSIPWNKLEMRRRM
                     LELLRVDGADSLLPKPQEPLTAEPFTENTAVMRGQPVKAVIEQDHMAHVKVHLMFILQ
                     PLIAQGPGMVGPALAALMDHVHEHILLDYEHVMKSAMLLAQQQLPGASPDQIAEQAAM
                     LGQESMSRFAQQLIPLMNEAMQVVQSKTPPPPQDPAIAATERTAQAQIAQKEKADQER
                     LKFDQQKFDREMQLREQESSIKPMLEQMQRQFDARLEVERMQREDDRAAFDRKVELLK
                     NDADNRQHQLTELLKNHEDNQTAIVIAQIKEALAAQPAPAAPEMPDFGPMMQQMQDML
                     GKIHEAKTNDALSATVEGLRAAIETMGRPKMIIKDASGKPIGIQ"
     gene            2782..>3193
                     /locus_tag="PLS35_19390"
     CDS             2782..>3193
                     /locus_tag="PLS35_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20891.1"
                     /translation="MNQNRRATDAAMSLDIDQMILEEEDKKQRAFLIVLNSINKSLIA
                     NTETIKEVSEKLDAHLTSYEQHVEQEDALLNKGRGAWKVMAWVISGVQVIGLGIWTMA
                     RGEIKEIHVMLQAEQKHLAQLESRVLFVERVADHK"
BASE COUNT          737 a          988 c          942 g          526 t
ORIGIN      
        1 cacggcggct ccgtttcaac ccccgttaca gtttccgacc tccgggctat aattgcaaca
       61 ttacgcatgg aacactccaa tgcctgaact cgatttcgac cagtctgccg ctccctctga
      121 cgtccctccc acctccctcg gggacattac ggaattggac gacggcagca tcctcgtcga
      181 ggaaccctcc gaggaggtcg tttcttctgc cgacgaccat tacgccaacc ttgcggaaac
      241 gctcgatccg ttcaacctga accgcatcgc caccgacctg ctcgaactga tcgagaagga
      301 caaggaggcc cgcaaggagc gtgacaaaca gcaggaagaa ggcatccagc gcacgggact
      361 gggcgacgac gcccccggcg gcgcgacctt tgatggtgct accaagaccg tacatcccgt
      421 gctggccgag ggctgcgtgg acttctccgc acgcgccatc aaggagctgt tcccgagcaa
      481 cggcccggtc aagaccaaga cactggggga agaaaccgcc gcgctgctca acaaggcgaa
      541 gcgcaagcgg gacttcctga acttctacat caccaaccgc atgccggagt accgctcgga
      601 gaaggaagtt ctcctgacgc aactcccgct aggcggttcg cagtacgaga aatactggtt
      661 cgacggcaag cgcgtgcgca tggagttcgt gccgatcgac aaggtctacc tgccgttcag
      721 tgccaacagc ttctacacct cgcaacgggt cacctacgag cgcgaagtta ccgaggaaac
      781 cgtccgccgg gaagtcgcca gcgggttcta ccgcgatgtg ttttcgacca tggctaccga
      841 ggagccggaa gaatcctcgt cgcaggccgc taccaataag gtggaaggaa agaccgcctc
      901 cgggtacaac gaggacggcg tccgcatcgt atacgaggtt gcctgcaact actccctcaa
      961 ggaagaggac gacccggcgc cgtatatcat ccacatcgac cagccgacca acaaggtcgt
     1021 ggcgatttac cgtaactgga agccctccga caacaccaaa gaaaagctcg attggtgggt
     1081 cgaggacaag ttcatcccgt ggcgtggcgc gtacggcatc ggcttgttcc atctggttgg
     1141 tggcatggcc gcctcgctca ccggtgccct gcgggcgctg ctggattcgg cgcatatcaa
     1201 caacgcgccg accgcgatca agttgaaggg cgggagaacc tccgggcaga acgtcaatgt
     1261 ggaggtaacg tccgttaccg aggtggaagc tccggcaggc gtcgacgaca tccgcaagat
     1321 catgatgccg atgccgttca acccacccag cacagtgctg ttccagttgc tggactggct
     1381 gacgaatcag gccaagggcg tggtagcgac cgccgaggag cgcattgccg acgccggtgc
     1441 gaacatgccg gtgggcaccg ccctcgccct tatcgaacag ggaagtcagg ttttcagctc
     1501 tatccacgca aggctgcacg actctcagcg tcgagccctc caaatcatct gtaggctgat
     1561 cgctgacttc cctgaccatg cgctggcgga tcttggccgt ttcgatctgg tgccggagga
     1621 tttcctcagc accgacgaca tcgaaccagt cagcgacccc aacatcttca gcgaaactca
     1681 gcgtttcgcc cagatgcaag cggttatgca gatggccgcc gccgacgccc ccgaccccag
     1741 catcccgtgg aacaagctgg aaatgcgtcg ccgcatgctg gagctgctgc gcgtggacgg
     1801 cgccgactcc ctgctgccga agccgcagga acccctcacc gccgaaccct tcaccgagaa
     1861 taccgctgtc atgcgtgggc aaccggtcaa ggccgtcatc gagcaggatc acatggcgca
     1921 cgtcaaggtg catcttatgt tcatactgca gccgctgatc gcacagggtc cgggaatggt
     1981 cggtccggca ctggcggctc tcatggatca tgttcacgag cacatcctcc tcgattacga
     2041 gcacgtgatg aagtccgcca tgctacttgc ccaacagcag ttgcccggtg cttcaccaga
     2101 ccagatcgct gaacaggccg ccatgctggg acaggaaagc atgagccggt ttgcccagca
     2161 gctgattccg ctgatgaacg aggctatgca ggtggtgcag agcaagaccc cgcccccgcc
     2221 gcaggacccg gccatcgcgg ctaccgagcg caccgcgcag gcccagatcg cccagaagga
     2281 aaaggcggac caagaacgac tgaagttcga ccagcagaag ttcgaccgcg aaatgcaact
     2341 ccgagagcag gaatcgtcca tcaagccgat gctggagcag atgcagcggc agtttgatgc
     2401 ccgcctcgaa gtggagcgga tgcagcgcga agatgaccgc gccgcatttg accgcaaggt
     2461 ggagctactg aagaatgacg cggataaccg ccagcaccag ctgaccgagt tgctcaagaa
     2521 ccacgaggac aaccagacgg cgatcgtaat cgcgcagatc aaggaagcgc tggcggccca
     2581 acccgccccg gcggctcccg agatgccgga tttcggcccg atgatgcagc aaatgcagga
     2641 catgcttggt aagattcacg aagccaagac caacgacgcc ctcagcgcca ctgtggaagg
     2701 tttgcgggcg gccatcgaaa ccatgggacg cccgaagatg attattaagg acgcttccgg
     2761 taaaccgata gggattcagt gatgaatcaa aaccgccgcg ccacggacgc agccatgtcc
     2821 ctcgacatcg accagatgat ccttgaggaa gaggacaaga aacagcgggc cttcctcatc
     2881 gtcctgaact cgatcaacaa gagcctgatc gcgaacactg agacgatcaa ggaggtgagc
     2941 gagaagcttg acgcgcactt gaccagctac gagcagcacg tcgagcagga agacgcgctg
     3001 ctcaataagg gacgtggcgc gtggaaggtg atggcttggg tgatcagcgg cgtacaggtg
     3061 atcggccttg gcatatggac gatggctcgc ggggagatca aagagatcca cgtcatgctg
     3121 caggcagagc agaagcacct ggcgcagctt gagtcgcgcg tactgttcgt cgagcgtgtg
     3181 gctgaccaca agg
//
LOCUS       DAOQEW010000322         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14903_length_3178_cov_8.162024, whole genome shotgun sequence.
ACCESSION   DAOQEW010000322 DAOQEW010000000
VERSION     DAOQEW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14903_length_3178_cov_8.162024"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..365)
                     /locus_tag="PLS35_19395"
     CDS             complement(72..365)
                     /locus_tag="PLS35_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNI20892.1"
                     /translation="MRMTVDQQVVAREWLARGFSCDLWVDPPGQVWEDYRHATDELVM
                     PVAGRLELEFEGQCFRPAPGEEIFIPACVSHTVRNIGGTTAKWIYGYKRVHTG"
     gene            complement(460..1464)
                     /locus_tag="PLS35_19400"
     CDS             complement(460..1464)
                     /locus_tag="PLS35_19400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNI20893.1"
                     /translation="MPATLVLERWAANWKMGSDVVPGAARLLWNATKQHAILGQILIM
                     TCGLAAHMEEALITPNVEHEPCWQPWLWLAREYGYRACWSFPVRTFGGPILGTLAWYF
                     KDPREPSLRMMELAATLTHAAAIIISRHREAEERTRVEAALRESDARYRAFVAVTSDI
                     VYKMSADWSEMQSLVGKEFIASTERPRRDWMDAYIPDADRTVVWEAIGAAIHSKQAFE
                     LEHQVIRLDGTIGWTYSRAVPLFDQKGEIVEWFGTASDITERKQAEAQLKTRNIELER
                     FNKVMVGRELRMMELNHKSPGRQGLQSESPLDQFTIEAQRPAAEPGQGDPGGESPRRA
                     "
     gene            1826..3091
                     /locus_tag="PLS35_19405"
     CDS             1826..3091
                     /locus_tag="PLS35_19405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20894.1"
                     /translation="MPTSGAQIERLRRKAEAYLRESAHTPPIDQSATETAQLVHELRV
                     HHVALRLQCEELRQTHLELEESRNRYVELYESIPVGYFSLNPRGAICDVNPAGAAMLH
                     QPMNRLIGRRFHLFMPVAQRRRFIEFCKQVCFERKRLSCEVDLGDASDDQPRALMNVL
                     LEGSPAQGQKDIMRLAVIDITRRKEAEQQLKVQEDELRTSRQKLQDLNAQLLNIQDEE
                     RRAIARELHDNCCQQLAMMMFSVNSIERCCDEPLTHKVRMLNEQIKRTLESVRHIAYG
                     LHPAMWDTMGVEEAMRSYLKDFIEVTELPVDFHAIDVPRRVPEVISTCLFRTLQEMLH
                     NVVKYAQASRVEVRLIKNENTLSLYVTDHGRGFHVKTMKQSPRGLGLVSLKERAQLLN
                     GTLNVTSQIGVGTMVHVILPLTSGNDHPM"
BASE COUNT          688 a          954 c          861 g          675 t
ORIGIN      
        1 ttgctctgtc cactcaccac aaattgcgtc gttgtcggca tcggctcctc tccctctgct
       61 gagttagctc atcagcctgt atgaacccgc ttgtacccgt agatccattt agcagtggtc
      121 ccaccgatgt ttcggactgt gtgggacaca caggccggaa tgaagatttc ctcgcccgga
      181 gcaggcctga agcactgccc ctcgaactcc agttccaacc ggcccgcaac cggcatcacc
      241 aactcatcgg tcgcgtggcg gtaatcctcc catacttgcc ctggcggatc aacccaaaga
      301 tcacaactga agccacgcgc cagccattct ctcgccacca cctgctggtc gaccgtcatt
      361 ctcatggtct ctcacccccg cccctggtca atcaggactg gctgttcagg gaaaaccaca
      421 tgtcagagag atcacgtagg tttgctgcca gaatgccggt cacgctcgac ggggagattc
      481 gccgcctgga tcaccctgcc ctggttcagc cgccggcctc tgcgcttcga tggtgaactg
      541 gtccagtggg gattcactct gcaacccctg ccgcccaggg ctcttgtggt tcaactccat
      601 catccggagt tcccgtccca ccatgacctt gttgaaccgc tccagttcga tattgcgggt
      661 cttgagctgg gcctcggcct gcttgcgctc cgtaatatcg ctggctgtgc cgaaccactc
      721 cacgatctcg cccttctgat cgaagagggg cacggcccgt gagtaggtcc agccgatcgt
      781 gccgtcgagt ctaatgacct ggtgctccag ttcaaaggcc tgtttggagt gaatggccgc
      841 gccgatggcc tcccagacga ccgtgcggtc ggcatcgggg atataggcgt ccatccagtc
      901 tcggcgcggc cgttcggtgc tggcgatgaa ctctttcccg accaagctct gcatctcact
      961 ccaatcggca ctcatcttgt acacgatgtc cgatgtgacg gcgacgaaag cgcgataccg
     1021 cgcatccgat tccctgaggg ccgcctccac ccgggttcgt tcctccgctt cccggtggcg
     1081 ggaaataatg atggcggccg catgggtcaa cgtggctgcc aactccatca tacgcaagga
     1141 gggttcacga ggatctttga aataccacgc aagcgtgccc agaatcggcc ctccgaaggt
     1201 tcgcaccgga aatgaccaac aggcgcggta gccgtattcg cgggccaacc agagccaggg
     1261 ctgccaacag ggttcatgtt cgacgttggg cgtgataagg gcctcctcca tgtgcgccgc
     1321 gagtccacag gtcatgatca ggatctgtcc caggatcgca tgctgtttcg tcgcattcca
     1381 aaggagccta gcagcaccag gcacgacatc tgatcccatc ttccagttcg cggcccatcg
     1441 ctcgagaaca agggttgccg gcatcgtatt gcgccgtgtt ggagatgacg cggtgaacgg
     1501 actgattccg atcgactatc atggcggtat tgcactgacg catgatccag tgaagagcaa
     1561 aatccaatga tcgatcgctt cgacgtgatt aaagtggcca acgtatagcc cgttgaatgc
     1621 tgagggtgac tctccgattg tacgtcccga acgaaccccg tagaatacag ctggtgtttt
     1681 acctcacagt tcattatccg gatctctttc ccggtatcgc agcggccatt gtcacctctt
     1741 gcgatcaaag ggctggtccc gtttaatttt ttcgaccggc cccagcattc tgaccacttc
     1801 atcctccgca ggtaaagagg acactatgcc gacatcaggg gcccaaatcg aacgcctacg
     1861 cagaaaagca gaagcctatt tgcgcgaaag tgcgcataca ccgcccattg atcagtccgc
     1921 aaccgagact gcgcagttgg tacatgaact tcgagtgcac catgtggcac ttcgccttca
     1981 atgcgaagag cttcggcaaa ctcatctaga attggaggaa agccgcaacc gctatgtgga
     2041 gctctatgag tccattcctg tcggatattt ttcgctcaat ccgcgcggag ccatctgcga
     2101 cgtgaatcct gccggagcag ccatgctgca ccagccgatg aaccgcttga tcggccggcg
     2161 gttccatctg ttcatgccgg tcgcgcaacg ccggcggttc atcgaatttt gcaaacaagt
     2221 ctgtttcgaa cgaaaacgtc tgagctgcga agtggatctc ggcgatgcct ccgatgacca
     2281 accacgagcc ctgatgaatg tgttgttgga aggcagcccg gcgcaggggc aaaaagatat
     2341 catgcgactg gcggtgatcg acatcacccg ccgcaaggag gctgagcagc aactgaaggt
     2401 gcaggaagac gaattgcgga ccagccggca aaaacttcaa gacctgaatg cgcagttgct
     2461 caacattcag gacgaggagc gacgcgccat cgcaagggag ctccacgaca actgttgtca
     2521 gcagttggcc atgatgatgt tctccgtcaa ttccattgaa cgctgttgcg acgagccgct
     2581 cacgcacaag gtgcggatgc tgaatgaaca gatcaagcga acgctggaat ccgtgcggca
     2641 catcgcctat ggcttgcatc cagccatgtg ggacacgatg ggtgttgaag aggcgatgcg
     2701 cagttacctg aaggacttca ttgaagtgac cgagctaccg gtagactttc acgcgatcga
     2761 tgtcccgaga cgggtgcccg aagtcatttc gacgtgcctc ttcaggacct tacaagaaat
     2821 gctgcacaac gtcgtgaaat atgcccaggc atcacgcgtc gaggtgcggc tcatcaagaa
     2881 cgagaacacc ttgtccctct acgtcacgga ccatgggcgc ggcttccacg tcaaaacgat
     2941 gaaacagtcc cctcgagggt tggggctcgt cagcctcaag gagcgtgcgc aattgctgaa
     3001 tgggacgttg aacgtcacca gtcaaatcgg agtcggcact atggtacatg tgatcctgcc
     3061 gctgaccagc ggcaacgatc atcctatgta aggacgatca ccttacctgc tccacctgtg
     3121 caagagttca tgtgttattc cttcctgtac gacatgggcg tggctactct tagacggc
//
LOCUS       DAOQEW010000323         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14936_length_3174_cov_9.437320, whole genome shotgun sequence.
ACCESSION   DAOQEW010000323 DAOQEW010000000
VERSION     DAOQEW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14936_length_3174_cov_9.437320"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            722..2005
                     /gene="hemL"
                     /locus_tag="PLS35_19410"
     CDS             722..2005
                     /gene="hemL"
                     /locus_tag="PLS35_19410"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNI20895.1"
                     /translation="MKTQRSEQLFAEAQQIIPGGVNSPVRAFRSVGGQPRFIARAKGA
                     RLYDVDGNSYLDYVLSWGPMILGHAPATVTKAIQQAAANGTSYGAPTELEIQLATMIR
                     EALPSMEQVRLVSSGTEAVMSALRVARAYTKRDGILKFEGCYHGHSDYLLAKAGSGLT
                     TLGIPDCPGVPEDFTKHTLTAPYNDLVTAEKLIKKHHRQLACVIVEPIAGNMGVVPPS
                     PDFLEGLRKLTDAHGMLLIFDEVISGFRVQYGGAQTLYGIKPDLTILGKIIGGGLPVG
                     AYGGAKDIMKMIAPSGPVYQAGTLSGNPLAVTAGIETLKRLKKPGTYEQLEQRSVALS
                     EGLGKIASKAGVPLTQTRVASMMCAFFTQGPVVDWATAKKSDTKVYAKFFHHMLDAGV
                     YLAPSQFEAAFMSVAHTPRDIERTLNAAQAAFKTL"
     gene            complement(2130..2363)
                     /locus_tag="PLS35_19415"
     CDS             complement(2130..2363)
                     /locus_tag="PLS35_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20896.1"
                     /translation="MDMFGKVGLVEVPILIAPDQKAWMDRMIKEGKIAIPPGGTLEKG
                     SLYSMFVRMLLHNAMEEQKRQEALDMDDEDDDE"
     gene            2581..>3174
                     /locus_tag="PLS35_19420"
     CDS             2581..>3174
                     /locus_tag="PLS35_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20897.1"
                     /translation="MRCTIVPDSRFLSRFALLLLVASPTTLAHGAVEESLSTLHTYTP
                     AIRNDPSPFHCTDCWDLKPDQRPSLPQHQSQRYRRGHHERGLRPHKNPFAKKSHSFSR
                     GMRSLPRHALSMGGFERTVEGWQVRTVDGDTIRYGTDRIRIRGYNAPELSEPGGREAA
                     LRLEQLLHDGIINIIPHGHDVYGRTLADVFVNGHNVAD"
BASE COUNT          712 a          948 c          842 g          672 t
ORIGIN      
        1 tccatctcgc acacaccagg cgcagagcac tctcactgac gtcactccat tcgttgcagt
       61 ccgactcctc cctgcaggcg caaaacccgc accaggaggc atgggctatc ggttcatcaa
      121 tttttcagag ggttagaaga aagagattgg cggaagggga agattcctgc agacaattgt
      181 gtggcaatat ggcgacaaat gacatttttt gtctcttccg aaaggtgagg tctcacactc
      241 atcattgcaa taaggcagac gctacttcag atcgtcggcg gatcaccgca ggctcatacc
      301 gttggcgcga tccctcttcg tagcaaggaa ccaggggatc ccgcccgatt cccatctgct
      361 gaagatggct ggcacaaatg ccgtgagttt cgcgtccatc ggccaaagga gctttttccc
      421 gcaccacagc cggtcgccct tcactttcac accaggagca aatcactttc atcatgttct
      481 cctacgcaat gacacccggt gttcagcaaa actcgcgcca ccaatcgaca ccaggaaaaa
      541 ccatgatttt taaaagaaac tctgattgaa ttcatgaagc ctcccggctg gtgtatcgat
      601 tcagatacca agctgtatcg gatcatctgc cggggcgcag ggaaaagcgg tgcatcaatt
      661 gaccgttttg aatttcccat gttaggttgc gccccaattc aaccctcgct gaaagaggcc
      721 catgaaaaca caacgttcgg aacagctttt tgcagaggca cagcagatca tccccggagg
      781 cgttaatagt ccggttcgag cctttcgctc agtaggcgga caacctcgct tcatcgcccg
      841 cgccaaaggc gcgcgcctgt atgacgtcga cggcaacagc tatctcgact atgtgctctc
      901 ctggggaccc atgattctcg gccatgcgcc ggccaccgtg acaaaagcca ttcaacaggc
      961 cgccgccaac ggcaccagtt atggcgcgcc gacggaattg gaaattcaat tggccacgat
     1021 gattcgagag gccctgccct caatggagca ggtgcgcctc gtgagttcgg ggaccgaggc
     1081 ggtgatgagc gccctccgcg ttgctcgcgc ctataccaaa cgcgacggca ttctgaaatt
     1141 cgagggctgt taccacggcc acagtgacta cctcttagcc aaagccggtt ctggtctgac
     1201 cactttgggt atccccgact gtcctggggt cccggaagat tttacgaaac acacgttgac
     1261 cgccccgtat aacgacttgg tgacggcgga gaagctgatc aaaaagcatc accggcagct
     1321 ggcctgcgtc atcgtcgagc cgatcgccgg caacatgggg gtggtccctc cttcgccaga
     1381 tttcctggaa ggcctgcgca agctgaccga tgcccacggg atgctgttga ttttcgacga
     1441 agtgatctcg ggattccggg ttcaatacgg cggcgcgcaa acgctctacg gtatcaagcc
     1501 cgatctgacg attctaggga aaatcatcgg tggaggactg cccgtgggag cctacggggg
     1561 tgcaaaggac atcatgaaga tgattgcccc gtccggtccg gtctatcagg ctgggacgtt
     1621 gtcaggaaat cccttggccg tcacggcagg catcgagacc ctgaagcggt tgaagaaacc
     1681 gggtacctac gaacaactcg aacaacggtc ggtcgcgctg tcggaaggat tgggaaaaat
     1741 cgcgagcaaa gcgggagttc cgctgactca gacgcgggtc gcctccatga tgtgcgcgtt
     1801 ctttactcag ggaccagtgg tggactgggc gactgccaag aaatccgaca cgaaggtcta
     1861 tgcgaaattc tttcaccaca tgctggatgc aggggtctac ctggcgccat cacagtttga
     1921 agccgccttc atgtcggtag cccatactcc gcgggacatt gaacggacac tcaacgccgc
     1981 tcaagccgcg tttaaaaccc tctgacctgg tgtgaagatg gcgggagttg ctctttgtga
     2041 agccggttcc cggatgcatt tcgtatcgaa actgatacga aatgcatcca ttcggatacg
     2101 cagctgaaga ccgatttcag cattcttgtc tattcgtcgt cgtcctcgtc gtccatatct
     2161 aacgcttcct gccgtttctg ctcttccatc gcattgtgca ggagcatgcg gacaaacatt
     2221 gaatacagcg aacctttttc caaggttccg ccgggaggaa tcgcgatctt gccttccttg
     2281 atcatgcgat ccatccaggc cttttgatcg ggcgcgatca aaatcggcac ctcgaccagc
     2341 cccacttttc caaacatatc catgatgtaa acctccgtga attctcactc attgttcgta
     2401 gctcgcggaa acccggcgag ctgacccgct cttccggcaa cactgcgctt cccgcgtcac
     2461 gacggacgtg cgtcggcatt tgagcacgcg gtttttcggc ttgtcaaccg aagagcccag
     2521 ccgtgactca tggcatggag cgtgctctaa cgagagctga accaacggag gacgttcagt
     2581 atgcgctgca ccatcgtacc cgactcccgc tttttgagcc ggttcgccct gctgttgctg
     2641 gtggcctctc ccaccacgct cgcacacggc gcagtggaag aaagcctgtc aacattgcac
     2701 acctacaccc ctgcgatccg gaatgatccg agcccgtttc actgcaccga ttgctgggat
     2761 ctgaagcccg atcaacgccc ctcgcttcct caacaccaat cccaacggta tcgacgcgga
     2821 catcatgagc gaggtcttcg cccgcataag aaccccttcg cgaaaaaatc gcattccttc
     2881 agtcgcggaa tgagatccct tccgcgccat gccctgtcga tgggagggtt cgagcgaacc
     2941 gtggaaggct ggcaagtccg cacagtcgat ggcgatacca ttcggtacgg cactgaccgg
     3001 attcgcattc gaggctacaa cgcgccggaa ttgtccgaac cgggcggaag agaagcggcc
     3061 cttcggctgg agcagctatt gcacgatggg atcatcaaca tcatccccca cggccacgac
     3121 gtctacggcc gcaccctcgc cgatgtcttt gtgaatggac acaacgtggc ggac
//
LOCUS       DAOQEW010000324         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_14961_length_3171_cov_8.701220, whole genome shotgun sequence.
ACCESSION   DAOQEW010000324 DAOQEW010000000
VERSION     DAOQEW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_3171_cov_8.701220"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2828)
                     /locus_tag="PLS35_19425"
     CDS             complement(<1..2828)
                     /locus_tag="PLS35_19425"
                     /inference="COORDINATES: protein motif:HMM:NF038336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HNI20898.1"
                     /translation="MAEKGRFAVFVDWKRNLLSRRQLGRPDRRALYQYRLTAAEFNDL
                     EGLLREWLGKLSRFELSQISRLTGFSGLFVLYAAEWWRHRFDGSHWSWDPILRDVGAE
                     PEEWNALQRSECVRLGLQEWGLVPRENGGLRFLGTVAVQGGLPLRLLAQARGKIGQLL
                     SQVLKQAGNSSVTHADLLTWVESLQGSLPRSYRQAAVYTLLADVAWIVLRLKEEARLT
                     SSADAILKLDQEVNGWRERFPLPIEDAHAQGLIEQLVRDAASVRIERHAICLPVERQL
                     VPDENGGWSLQSNVALPDSIQASQLAKLFAVAAEDLPRAGELALTAGSHRLMTTIRRM
                     AGHDGYRMERKPWGYSGATTAHEHVLHLSAPDGRAWSGIAPKGEMLDEELPWVFSAEE
                     SSYRFLRQGGGGVAATEALVALPAGWNVRPTEGSEASACGRLDSPDRELLRVRGVVEA
                     HDGTGLSCRIRTGQAGSAEDSYEWRGQRIWLDFQSPTMAFKGLPNLYRINQDGAVHKV
                     DGNLGWSVIGAPAANDLRPIGPIAIRYPATGEVKQRARMVALPADAALSMVSRDATSG
                     NIQLDKWAVTAVRVITDGVRHESRRASDAWILSLSVAPGARTPERIEIEVFWRQTMTP
                     VRVTLPFPGRGARAFDASGTELRSGSLLAADQLAGVRMLILDGGSNAHMTLEIKSSHC
                     DRLRKHKLCALPGAVSVEVRLQDYATDVQHLLSTDDSPDARVRIELRIGGSDPYRLDV
                     ARYAAKLVKNNSEIGLDDGGSAMLTPEEISSLPVMALRLERPGDEAIRLTPCTSEGVP
                     TGSWAFAPETREPGCWLIYPGPDAQLRFRPTLWTIAGEVLSDSPLAHAIGIADQSERE
                     SALDAVIDMIAADFLKPCWIEVERLAGQIGHLPLATLDIWRRFARSAKGMAALAIRFG
                     TLPSGFLDRFDQELPFAWEAVPFEAWTQA"
BASE COUNT          622 a         1032 c          922 g          595 t
ORIGIN      
        1 gcctgcgtcc acgcttcgaa gggcactgcc tcccacgcaa acggcagttc ctgatcaaac
       61 cgatccagga agccgctggg gagagtgccg aatcggatgg ccagggccgc catgcccttc
      121 gcggaacggg cgaagcgacg ccaaatatcc agagttgcaa ggggtaggtg gccgatttgc
      181 cccgccagtc gttccacttc aatccagcaa ggcttcagaa aatccgctgc tatcatgtca
      241 atcactgcat cgagggcgga ctcgcgctcg ctctggtcgg caatgccgat ggcgtgggcc
      301 agcggactgt ccgacagcac ctcgccggcg atcgtccaaa gcgtagggcg aaaacgtaat
      361 tgcgcatcag ggccggggta gatcagccaa catccgggct cgcgggtctc tggcgcaaat
      421 gcccatgaac cggtgggcac cccctcggag gtgcagggag tcaatcgaat ggcttcgtcg
      481 ccaggtcgct ccaggcgcag tgccatcacg ggcagagagc tgatctcctc cggggtgagc
      541 atcgcgctgc caccatcgtc gagcccgatc tccgagttgt tcttgaccaa cttcgcggcg
      601 tagcgggcca catcgagccg gtatgggtcg ctaccaccga tccgcagctc gatccgcact
      661 cgcgcgtcag ggctgtcgtc ggtggagagc aaatgctgga cgtcggtcgc gtagtcctgc
      721 aatcgaacct cgacgctgac cgcgcctggg agtgcacaga gcttgtgctt gcgcagacgg
      781 tcgcaatggc tggacttgat ttccaacgtc atgtgagcgt tgcttccacc gtcgaggatc
      841 aacatgcgaa cgccagcaag ttgatcggcg gcgagcagcg agccggaccg cagttccgtg
      901 ccgctggcat cgaaagccct cgcccctcgc cccggaaaag gtagggtcac ccgaaccggg
      961 gtcatggttt gtcgccagaa aacttcgatc tcgatccgtt caggcgttcg cgcaccagga
     1021 gcgacactca gtgagaggat ccatgcgtca cttgccctcc gcgactcgtg ccgaactccg
     1081 tcggtgataa cccgcacggc agtcaccgcc catttgtcga gttggatatt cccggatgta
     1141 gcatcacgac tgaccatcga cagtgcagcg tctgctggta gtgcaaccat tcgtgcacgt
     1201 tgtttcacct cgccggtagc cggataccgg atggcgatcg ggccaatcgg ccgcaggtcg
     1261 ttcgctgctg gggcgccgat cacgctccaa ccgaggttgc cgtccacctt gtgaacagct
     1321 ccgtcctggt tgatccgata gagattgggc agccccttga aagccattgt ggggctctga
     1381 aaatccagcc aaatgcgctg tccccgccac tcgtagctgt cttctgccga accagcttgc
     1441 cccgtacgta tgcgacatga gagccctgta ccgtcatggg cttcgacaac gccgcgaacg
     1501 cgaagcaact ctcgatcggg cgaatccagt cggccgcaag cggacgcctc ggacccttcg
     1561 gtgggccgga cgttccatcc cgccggcagc gccaccaggg cctcggtggc ggccacaccc
     1621 ccgccacctt gacgtaggaa gcggtatgag gattcctctg ctgaaaacac ccaaggcagt
     1681 tcctcgtcga gcatttcgcc cttgggagcg attccagacc aggcccggcc atccggtgca
     1741 gaaagatgca gcacgtgctc gtgagcggtg gttgctccgg aataccccca gggcttccgc
     1801 tccattcggt agccgtcgtg tcccgccatg cgacgaatgg tcgtcatcaa ccggtggcta
     1861 ccggcagtca aagccaattc ccccgcgcgc ggcaagtctt cagccgccac ggcgaagagc
     1921 ttggcgagct ggcttgcctg gatgctgtcc ggaagggcga cgttagattg cagcgaccac
     1981 ccgccattct cgtcgggcac aagttggcgt tccaccggca ggcagattgc gtgacgttca
     2041 atgcgcacac tggccgcatc tcgaacgagc tgttcgatca agccttgcgc gtgggcatcc
     2101 tcaatgggta gcggaaaccg ttctcgccag ccgttgacct cttgatccag cttgaggatc
     2161 gcatcggcgc tggacgtgag cctcgcttct tccttcagac gcaagacgat ccaggccaca
     2221 tcggccagca aggtgtagac ggccgcctgc cgatagcttc tcggcaagct gccttgcagg
     2281 ctttcaaccc aggtcagaag atcggcatga gtgacgctgc tattgccagc ctgtttgagg
     2341 acctggctca gtagctgacc gatctttccc cgggcctgcg ccaacaaccg caacgggagg
     2401 ccaccctgta ccgccacggt gccgagaaaa cgcagcccgc cattttccct gggcaccaag
     2461 ccccattcct gcagacccaa gcgaacacat tcactccgct ggagcgcatt ccattcctcc
     2521 ggctctgccc caacatcgcg caggatggga tcccaggacc aatgagatcc gtcgaatcgg
     2581 tgccgccacc actctgcggc gtaaagcacg aacagtccgg aaaaccctgt caggcggctg
     2641 atctgcgaca gctcgaagcg ggataatttg ccgagccact cgcgcagcag gccctccaga
     2701 tcgttgaact ccgccgcagt aaggcggtat tggtacagcg cacgtcgatc tggtcgcccg
     2761 agttgtcggc gggatagcag attgcgtttc caatccacga ataccgcgaa tcgacccttc
     2821 tccgccatcc gcccccctcg gaaattgagt tataggttca tcgtcataac cgccgcacgc
     2881 cctgaagaca agccatatgt tgcggctgat ccactccgac cagaagtact gatccttctc
     2941 cctgtatgag cttgttgccc acccaatcga aacagccggt ccgctgcgcg cagtcacatg
     3001 cgcgtcgggt cacaaaccgc catcgcgaag atcgatggca agctgccgac atgggtgata
     3061 ttgtaaaggt ttgacgaagt cagcgtcata tctcacaatt cctcgcgctc tgcgggttgc
     3121 gtcaccgccc gagcagcgcg gcggcggcat ccatctgctt gtgctcgaag g
//
LOCUS       DAOQEW010000325         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_15492_length_3105_cov_8.313770, whole genome shotgun sequence.
ACCESSION   DAOQEW010000325 DAOQEW010000000
VERSION     DAOQEW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_3105_cov_8.313770"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLS35_19430"
     CDS             <1..763
                     /locus_tag="PLS35_19430"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI20899.1"
                     /translation="MSLLDQFFVYHPHPWEERDWSAVGGVPLEDIWFQAIDGTKLFGW
                     YAENPATSAVLLWCHGNAGNMTHRLENLAALYRLGLSVFLFDYRGYGKSHGRPSEDGL
                     YRDALGAYDYLTKVRRVRPERVVIFGRSLGGAVAGELATQRAAMGLLLESCFPSIEAV
                     ARHHYMGLPMHWLLGAAFRLDNHLPHLSLPKLFVHGDRDDIIPVELGQQAFAAAKAPK
                     EWYVVRGADHNDVPSVGGRAYFAKLFAFIHAAIGR"
     gene            1022..1972
                     /locus_tag="PLS35_19435"
     CDS             1022..1972
                     /locus_tag="PLS35_19435"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNI20900.1"
                     /translation="MEVVSAVLVLFVVGCAGGPAQTTRQAGSGMESVAQGELRTEGTK
                     PVQVAASPAVSSNMVVLVANKAASPAAEKEPADQPEEDPFFDPFAKSDEPAGGEEYDP
                     WEPVNTKIFEFNRQVDRWVLKPAARGYNAVVPNPVQIGISNLFYNMRFPSRLINNLAQ
                     GKFSGAGTEVGRFLLNSSFGLGGLVDVAKYMDITTPEEDTGQTLGYYGVKPGPYVVLP
                     FLPPFTLRDLVGYVGDIALNPINWLVFPIIEINGVPSLVAHHNRTTSSIAQISGRVEE
                     ILNDRSLNLEKFQGVEEATLDLYTAVKNAYIQKRRNAIRE"
     gene            complement(1976..>3105)
                     /locus_tag="PLS35_19440"
     CDS             complement(1976..>3105)
                     /locus_tag="PLS35_19440"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNI20901.1"
                     /translation="LYKHERYILGPQSSEIRVAQGEVVNLCANNYLGLANHPDVRRAA
                     ADGLKDYGYGMASVRFICGTQDLHKRLEQSISTFFGTADTILYSSCFDANGGLFETLL
                     DERDTIISDALNHASLIDGIRLCKASRLRYAHADMEDLEARLAESSASRVRMIVTDGV
                     FSMDGDLAKLDRIADLADRYDAAVVVDDSHATGVLGKGGRGTPDHFGVAERIDLVTST
                     LGKALGGATGGFTTGRKELIELLRQRSRPYLFSNSLPPVIAAAALKAIALVEQGDHLR
                     MNLMEQAGWLRSQLAALGFTLVPGHHPIIPVMLGDASIATKMADRLLQEGIYVVGFSY
                     PVVPKGQARIRLQLSAAHTRAQLERAVAAFATVGRELHVIN"
BASE COUNT          630 a          860 c          950 g          665 t
ORIGIN      
        1 catgagcctg ctggaccaat tttttgtcta tcacccgcat ccgtgggaag aacgcgactg
       61 gtctgccgtc ggcggggtgc cgttggagga catttggttt caggccatcg atggcacgaa
      121 actcttcggc tggtatgccg agaatcccgc cacctctgcc gtgttgctgt ggtgtcatgg
      181 taatgccggc aatatgaccc atcgattgga gaatcttgcc gcgctctatc gcctgggatt
      241 gtcggtgttt ctattcgact atcgaggtta tggaaaaagc cacgggcgcc cgtcggaaga
      301 cggtctctat cgggatgcac tgggcgccta cgactacctg acgaaagtgc gtcgagtcag
      361 gccggaacgg gtggtcatct tcggacgctc attggggggc gcggtagccg gagagctggc
      421 gacgcaacgc gcggctatgg ggctcttgct ggaatcctgc tttccttcaa tcgaagcggt
      481 tgcgcgtcac cattatatgg ggttgcccat gcattggttg ctgggcgccg ccttccgatt
      541 ggacaaccat ctgccgcatc tttcgttgcc gaagttattt gtgcatggcg accgcgacga
      601 catcattccg gtggaactcg gccaacaggc gttcgccgct gccaaagctc ccaaagagtg
      661 gtatgtggtg cgcggagccg atcacaacga tgtgccgtcg gtcggagggc gagcctattt
      721 tgccaagctg ttcgccttca ttcacgctgc cattggtcgt tgacctggta aaaaatctcc
      781 gcatcttctg tattttttct aggcagattc agctcgcttg gtaagatgcg atggagcggt
      841 caggcataag gccaggagat cggcatcaga gggcgtcctc atctggcagc tgtccgtata
      901 ttctgctaga gtgcgtccgc ctgcgcgccc tgaagacgcg cggtggcaac gttcaatcag
      961 cgtgaaaagc cctcggcgag gaggcaaggt gcatcggaca ggagtgcatg caagaaggtt
     1021 cgtggaggtg gtgtctgcag tgttggtcct cttcgtagtg ggatgtgccg gcggccctgc
     1081 tcaaactacg cgacaggccg gtagtggaat ggaatcggtc gcccaaggag agttacgaac
     1141 cgaagggacg aagccggttc aggtcgccgc gtctccagcg gtctcgtcca acatggttgt
     1201 gttggtggcc aataaggccg cttcgcctgc agccgagaag gaaccggcag accaacctga
     1261 agaggatccg tttttcgacc cctttgccaa gagcgacgag cctgcagggg gtgaagagta
     1321 tgatccttgg gaaccggtga ataccaaaat cttcgagttc aatcggcagg ttgaccggtg
     1381 ggtgctcaag ccggctgcgc gaggatacaa tgcggtggta ccgaacccgg tgcagatcgg
     1441 gatcagtaac ctcttttaca acatgcggtt tccttcccgc ctgatcaaca atcttgcgca
     1501 ggggaaattc tccggtgcag gcaccgaggt cggccggttt ctcctgaata gcagcttcgg
     1561 attgggcggg ctggtggatg tggcgaagta tatggatatc accacgccgg aagaagatac
     1621 cggtcagacg ctgggctatt acggggtgaa gccggggccg tacgtcgtat tgccatttct
     1681 gccgccattc acgttgcgcg atctcgttgg ctatgtcgga gacattgcgc tcaatccgat
     1741 caattggctg gtctttccca tcattgaaat aaacggcgtt ccttcgctgg tggcccatca
     1801 caatcggacc acgtcgtcga tcgctcaaat cagcggccgc gtggaagaaa ttctgaatga
     1861 ccgctcgttg aacctggaga agtttcaagg cgtggaggaa gcgactctgg atttgtacac
     1921 agcggtgaaa aacgcctaca tccagaagcg gcgcaacgcc atcagggaat agcgactagt
     1981 tgatgacgtg gagctcgcgc cccacggtgg cgaaggcggc gacggcccgt tcgagttggg
     2041 cgcgggtgtg ggccgcggag agttgcaggc gaatacgcgc ctgtcctttt ggtacgaccg
     2101 gatagctgaa tcccaccaca tagattcctt cctgcagcag gcggtcggcc atttttgtgg
     2161 cgatcgaggc gtctcccagc atgaccggaa tgatgggatg atggcccggt acgagggtga
     2221 agccaagcgc agccagctgg ctccgcagcc agccggcctg ctccatcaag ttcatgcgta
     2281 aatggtcgcc ttgctcgacc aaggcaatag ctttcaacgc ggcggccgcg atgaccggcg
     2341 gcagactgtt cgaaaacaag tagggacgcg accgttgccg taacagctca atcaattcct
     2401 tcctgccggt ggtaaacccg cccgttgcgc caccgagcgc cttgcctagg gtgctggtga
     2461 caaggtcgat ccgctcagca acaccaaaat gatcaggggt gccgcgaccg cctttcccca
     2521 agaccccggt cgcatggctg tcgtccacaa ccacggctgc gtcatagcgg tcggccagat
     2581 cggcaatgcg atccagtttc gccaggtcgc cgtccattga aaataccccg tccgtgacaa
     2641 tcattcgcac ccggctcgca gaggattccg ctagtcgcgc ttccaggtcc tccatgtcgg
     2701 catgggcata gcgcaggcgt gaagccttgc agagacgaat gccgtcgatc aggctggcat
     2761 ggttcagggc atcgctaatg atggtgtccc gctcatcgag caatgtctcg aacaatccgc
     2821 cgttggcgtc gaagcaggag ctgtagagaa tggtgtccgc agtgccgaaa aaggtgctaa
     2881 tgctctgctc gagtcgcttg tgcaagtcct gcgtcccgca aataaaacgc accgaggcca
     2941 taccgtatcc gtagtccttc agcccatccg ccgccgctcg ccgaacatca ggatggttgg
     3001 ccagcccaag atagttattc gcacagaggt tgacgacctc accctgggcg acgcggatct
     3061 cgctgctttg cggacctaaa atgtatcgct cgtgtttgta gagac
//
LOCUS       DAOQEW010000326         3100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_15532_length_3100_cov_7.013793, whole genome shotgun sequence.
ACCESSION   DAOQEW010000326 DAOQEW010000000
VERSION     DAOQEW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15532_length_3100_cov_7.013793"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLS35_19445"
     CDS             complement(<1..933)
                     /locus_tag="PLS35_19445"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNI20902.1"
                     /translation="MQFSWRVQLRVLYLIIMFFQSKKIPCSSQLAEPQAQYAALQREN
                     ESLHAELSIAMADIDRIRGEYQSLNEMLSNLVDFKKSLDKSQISLGNAATSLREERAL
                     AQVVAKMAIDSGETTTNIATSLQRLAQDSSATAQEVGTLAGRVDEISSIVQLIHAIAD
                     QTNLLALNAAIEAARAGESGRGFAVVADEVRKLAERTTKATKEIGTLVVGIRQNSSTA
                     EQAMCILSDAAANFSKRGDQATNSMEQLVNLSQRMEKIITNRSFKNFVEVVKVDHLVF
                     KLNVYMKIFGFDLPNLEKLAPHTGCRLGKWYYEGD"
     gene            1262..2325
                     /locus_tag="PLS35_19450"
                     /pseudo
     CDS             1262..2325
                     /locus_tag="PLS35_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235908.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2346..>3100)
                     /locus_tag="PLS35_19455"
     CDS             complement(join(2346..2767,2767..>3100))
                     /locus_tag="PLS35_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611167.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNI20903.1"
                     /translation="AMERRALNDRQWALMAPHCLGKPGDAGRRGADNRLFVEAVLWIV
                     RTGSPWRDLPAEFGKWNTVFKRFRDWVKADVFHKLFEAVSQEPDMEYAMVDATIVKVH
                     RHGQGAKGGPESQAIGRSRGGMSTKILALCDALGNLVRFVLMPGQRHDSKGVQELIDG
                     IEFGALLADKAFDANWLIEELEKRGAQVVISQMPGRCEPLQIDEHMYRWRHLIENFFA
                     KLKEFKRITMRSDKTDTSFTAAIYLASAVINSR"
BASE COUNT          644 a          875 c          889 g          692 t
ORIGIN      
        1 gtcaccttcg taataccact tgccgagtcg gcaaccagtg tgaggagcca gtttttcgag
       61 gttgggtaag tcgaagccaa agattttcat ataaacattc agcttgaaaa ccagatgatc
      121 gactttgacc acttcaacga aattcttgaa ggagcggttg gtgattatct tctccatccg
      181 ctggctcaaa ttcactaatt gttccatgct attggtggct tggtcccctc gtttgctgaa
      241 attagccgct gcgtcggaca ggatacacat tgcctgttcg gcagtagatg agttttgtcg
      301 aatgccgaca accaacgtcc cgatctcttt cgtggctttc gtcgttcgtt cagcaagttt
      361 gcggacctcg tcggcgacga cagcgaagcc gcgtcctgat tctccggcac gagccgcttc
      421 gatggctgca ttcaaagcca acagattggt ctggtcggct attgcgtgaa tcaactgaac
      481 gatagagctg atttcatcga cccgtccggc caaggttccg acttcctgag ccgtggctga
      541 ggaatcttgc gccaaacgct gtaggcttgt ggcaatgttg gtagtcgttt cgccgctgtc
      601 gatcgccatt tttgcgacta cctgggccag agctcgctct tctcgcagtg aggtggccgc
      661 attgcccaga ctgatctggg atttgtccaa tgatttttta aagtccacca gattgctaag
      721 catctcatta agcgactggt attcccctct aattcgatcg atatcggcca tggcgatgga
      781 taactcagca tgaagcgatt cattttcgcg ctgcaacgcc gcgtactgcg cctgcggttc
      841 agccagttgc gacgagcatg gtattttttt tgattggaaa aacatgatta ttagatataa
      901 aaccctaagt tgaacccgcc aggagaactg catggaaaat tgagttaatt gaaaaatatt
      961 agatcagcgg ctttcgttat acaactgaat ggcgccggtg tagtgtattg tcatcatccc
     1021 ggggggcggg ctggttgtcg gtggagctta tgggcgaagg gacgcgcggt gggaaagtcg
     1081 ccagactttt ccacgacaag cggcccggtg cgcgacagcg catcgtccac aagtccacgt
     1141 agtcccttcc tcatgcctta cgctgcgggc cgtatagcgt gcgtcggggt ctagcgcata
     1201 tccttataca caacttaagg atctggctgt aagctgccga tttttttgag aaggtggcag
     1261 gatgggctgg gctgcggagg aactgaagga agttgatctg ggggaccgaa gactgaacaa
     1321 gcgagcgatc gcgttgttgg acacgcggtc tgctagaccg acggcgagta tcccgtcggc
     1381 gtgctctggc tggtcggaga cgatcgcggc ctaccgcttc ctggacaatg atgaggtcac
     1441 gtgggagggc atcctgagac ctcattcctg agacctcatt ggtcgtgctc tcggacgcgg
     1501 atcgcctgcc acaaggtcgt gctgatgttg caggacacga ccgaactgga cttcaacggg
     1561 ccacgcatcg acggcttggg tcccttgagc tatgaagcgc agcgcggtct gtacgtgcac
     1621 ccgacgtacg ccgtcagcac ggatcgcgag cccttggggg ttctggatgc gtggatgtgg
     1681 gcgcgcgagc cgaaggatgc cgacgggcaa catccgggga tcaaagagag catgcgctgg
     1741 gtcgagggct atgagcgagt cgctgaactg gcaggggagt tgccggacac ccggctggtc
     1801 tacgtggcgg accgcgagtc ggacatgatc gaactgatgg cccgcgctga cgacttggag
     1861 accccggccg actggttgct gcggtcgccg cacgatcgag cgcttcccgg cggccagacg
     1921 ctgtggcaaa cggtgacctc gggtgatgaa ctcggcggaa tctgttttac gctgccgtcg
     1981 agacagggcc agaaagctcg cctggtgcgc cagcggattt gggcgagggt cgtcgaacgt
     2041 cctcatggca agccgcaggc catccgagcc accggagtgg tcgccaagga gatcgatccg
     2101 ccggccggca gccccccggt ggagtggcgc ctgctgacca atcgcaccgc cgagacgctg
     2161 gccgaggtgg cggaacggat tgactggtat cgcgcacgct gggagatcga gagcctcttc
     2221 cacatcctca agaacggctg ccgaatcgag tccctgcaat tgggaacgat cgacggcctg
     2281 caacgcgcca tcgcgctgta cctgattgtt tcctggcgca ttgccatgct agggtgtgtt
     2341 gagaatcacc gggagttgat cacggcagaa gccaagtaga tggctgccgt gaagctggta
     2401 tccgttttgt cgctgcgcat ggtaatgcgt ttgaactcct tgagtttggc aaagaagttc
     2461 tcgatcaggt gacgccaccg atacatatgc tcatcgattt ggagtggctc gcaccgaccc
     2521 ggcatctgtg agatcacgac ctgcgcacct cgcttttcaa gctcttcgat cagccagttg
     2581 gcatcgaacg ccttgtcggc cagtagcgcc ccaaactcaa tgccgtcgat caactcctgc
     2641 acacccttgc tgtcatggcg ctgacccggc atcaacacaa acctcaccaa gttgcccaac
     2701 gcatcgcaca aagccaaaat cttggtgctc atgccgccgc gcgagcgtcc aatggcctga
     2761 ctttcagtcc cccttttgcg ccctgtccgt gacggtgaac cttcacgatc gtggcatcca
     2821 ccatcgcata ctccatgtcc ggctcctgac tgacagcttc aaaaagcttg tgaaacacat
     2881 cggccttgac ccaatcgcga aagcgcttaa acaccgtgtt ccacttgcca aactcagcgg
     2941 gcaagtcgcg ccaggggctg ccagtgcgca caatccacag cactgcttca acaaacaaac
     3001 ggttgtccgc accccttcgt cccgcatcac cgggctttcc aaggcaatgc ggcgccatca
     3061 acgcccactg cctgtcattc aacgctcttc tttccatagc
//
LOCUS       DAOQEW010000327         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16097_length_3039_cov_6.904491, whole genome shotgun sequence.
ACCESSION   DAOQEW010000327 DAOQEW010000000
VERSION     DAOQEW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16097_length_3039_cov_6.904491"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLS35_19460"
     CDS             <1..750
                     /locus_tag="PLS35_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20904.1"
                     /translation="NQPGGELSGIMKTPSTEGYLRYSLTALATNSFSGPLGGCPPNTP
                     CGTGGRNVNFYGHITQSFGGYGIVTGNRVGIFGAYGQAPTMVNATCPTCQGVAGSGKP
                     FTRIGVDVSLTFNGEWNIFGAIMHGHDSAQLFASQGIPTPQNASWNGAFVELDYAPAL
                     FFDMPDWFFAYRYDVIRNDRHGDGNTDVFKGNYNDVDSHTVLARYFIHHSTRTDLALH
                     AEYNSYRTQAVGPTGGDLRGQTMLVGLDFAY"
     gene            862..1605
                     /locus_tag="PLS35_19465"
     CDS             862..1605
                     /locus_tag="PLS35_19465"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNI20905.1"
                     /translation="MAPGTERPCFSCDDQRLVRMVPLEKGTHQKLLLRHPLVLQQQEW
                     EALLRMVKVRSIHRPLLGATYQGDTEPLFSEEEVRYLGDSLQRAFQQATPQEHVVFAL
                     ARPSQTGLEQITSGAWFVEDGLIHLRVANCRIAVTMPSIRKQIWKDPLFAQAGAFYEF
                     VPGGGQAIARATVDGGNPFRSDPDELLIEYKVASSANPAPVSSEPSAQPVPPRSLEEQ
                     LSLLKRLLEQGLITEEDYRTKKQQLLDRL"
     gene            1697..2506
                     /locus_tag="PLS35_19470"
     CDS             1697..2506
                     /locus_tag="PLS35_19470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI20906.1"
                     /translation="MSRSSASPSTRWPLPAQDDWSTPFAELLLAQLDLHSGLTILDIA
                     CGHGIPAFYLAEHVGPTGSVLGIDMSRGQVANARTVQRGELPWLRFECVDMRALPPSF
                     PTFQRITGNLSVMFLRPHRFEAMRGLIAHLEPGGQIVLTFPSLGTFDSMWQRIDREMS
                     RHGLTMERERLAAHVAERPSAAEARGWLEQLKMERIDVIEQPLEVPSGAGQRFLQHPL
                     LRGGFLDDAYECFDDARLADTVMTSVAADLESMMPLVARRCALSGWKPRQA"
     gene            2547..>3039
                     /locus_tag="PLS35_19475"
     CDS             2547..>3039
                     /locus_tag="PLS35_19475"
                     /inference="COORDINATES: protein motif:HMM:NF015782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HNI20907.1"
                     /translation="MNGLLTRLLACSASLILVTACSTLPSKYIEKAEPGVTLTSLAAA
                     PETYQGKTVILGGVVVDQQQQGQRLWLHLKNRPLDKDYRPHRPTINEGPEAGYYWVVV
                     PDASLLPPKWKQWARVTVVGRVMDQRETRPTTVPTSEPVLSLVFMRGWAMGNAQGKGA
                     WEES"
BASE COUNT          590 a          849 c          969 g          631 t
ORIGIN      
        1 aaccagcccg gcggagaatt gtcaggcatc atgaaaacgc cgtctaccga aggatatctg
       61 cgctattcgt tgaccgccct ggcgacgaat tcgttttcgg ggccgctcgg gggatgccct
      121 cccaataccc catgcgggac gggcgggaga aacgtgaatt tctatggtca catcacgcaa
      181 tcgttcggcg gctacggcat cgtgaccggg aatcgagtgg ggatcttcgg ggcctacggc
      241 caggccccca cgatggtcaa tgcgacctgc ccgacctgtc agggcgtggc gggaagcgga
      301 aagccgttca cgagaatcgg ggtcgatgtg agcctgacct tcaacggaga atggaacatc
      361 ttcggggcga tcatgcatgg acatgacagc gcccaattgt tcgcctcgca agggattccg
      421 accccgcaga acgcctcgtg gaacggcgcg tttgtcgaat tggactatgc gccggccctg
      481 ttcttcgaca tgccggactg gttttttgcg tatcgctatg atgtgatccg aaacgaccgg
      541 catggcgacg gcaacacgga tgtcttcaag ggcaactaca acgatgtgga ttcccatacc
      601 gtgctggccc ggtatttcat tcatcactcc acgcgaaccg atttagcctt gcacgcggaa
      661 tataatagtt atcgcacgca agccgtcggc ccgaccggag gtgatttgag agggcaaacc
      721 atgctggtgg gattggattt tgcgtattga gaatcgtctg cccagaagaa aggttatagt
      781 tctgtgaacc atccgtttcg tcgcggggca actgtcgctg tgctatggct ttcgtgtctc
      841 gccgtctcag gttgttcggg catggctccg ggcactgagc ggccttgctt ctcctgcgac
      901 gaccagcggc tcgtcaggat ggttccgctc gagaaaggaa cccatcagaa gctgctgctc
      961 cgtcatccgt tggtgctgca gcagcaggag tgggaggccc tgctgcgaat ggtgaaggtg
     1021 cgaagcattc accggcctct gctcggcgca acctatcagg gagacacgga accgctgttc
     1081 tccgaagaag aagtccggta tctgggcgat tcactgcagc gagcctttca gcaggcgacc
     1141 ccgcaggagc acgtggtgtt tgcgctcgcc aggccgtcgc agacgggatt ggaacagatc
     1201 acctcgggag cctggtttgt cgaagatggc ctcatccatc tccgtgtggc gaactgtcgg
     1261 atcgcggtga ccatgccgtc gatcaggaaa cagatctgga aagatccctt gtttgcgcag
     1321 gccggggcgt tttatgaatt cgtgccaggt ggagggcagg cgatcgcgcg agcgaccgtg
     1381 gacggcggga atccgttccg ttccgacccc gacgaactgc tgatcgagta caaggtcgcg
     1441 agctctgcga atccggctcc ggtctcttct gagccctctg ctcaaccggt tccgccacgc
     1501 tcgctggagg agcaactcag cttgctcaaa cgccttctgg agcagggttt gattacggag
     1561 gaggactatc gtacgaaaaa gcagcagctg cttgaccggt tgtgagcgtg aaggtgaccg
     1621 aagagatttc tgacttgtcc tttacccttc gggtaagatg cggctgagag ccatcacccc
     1681 cggcgaaagg aatgtcatga gcaggtcctc cgcatccccc tctacgcgct ggccccttcc
     1741 tgcgcaggat gactggtcca caccctttgc ggaactgctc ttggcgcaac tcgatctgca
     1801 ttccggtctc accatcctgg atatcgcctg cggccacggc attccggctt tttatctggc
     1861 cgagcacgtg gggccgaccg gctccgtcct cgggatcgat atgagtcgcg ggcaggtggc
     1921 caatgccagg acagtgcaga gaggtgagtt gccctggctc cgcttcgaat gtgtcgacat
     1981 gcgggccttg cccccatcct ttcccacttt ccaacggatc accggcaatt tgtccgtcat
     2041 gttcctccgg ccgcaccggt tcgaggcgat gaggggactg attgcacatc tcgagcccgg
     2101 cggccaaatc gtgctgacct ttccctcgct cggcaccttc gactccatgt ggcaacggat
     2161 tgatcgagaa atgagccggc atgggttgac gatggagcgt gaacggctcg ctgcgcatgt
     2221 ggcggaacgg ccgtccgcag cggaggcgcg tggttggctc gaacagctga agatggagcg
     2281 gatcgacgtg atcgagcagc cgcttgaagt gccgagcggg gccgggcaac ggtttctcca
     2341 gcatccgctg ttacgcggtg gatttctcga cgatgcctat gaatgtttcg atgatgcccg
     2401 gttggccgac acagtgatga ccagcgtcgc ggcggatctt gagagcatga tgccgctggt
     2461 ggcgaggcgg tgcgcgctca gtggctggaa gccgcgtcag gcctgattgc ttcggtgcat
     2521 cgaggttgcg acgaacggag gacgatgtga acggtctgct gactcgatta ctggcatgtt
     2581 ctgcgagtct catactcgtg acggcctgta gtaccttgcc atccaagtat attgagaagg
     2641 ccgagccggg agtcaccttg acctctcttg ccgccgcgcc ggaaacatat caaggcaaga
     2701 cggtcatcct gggtggggtg gtagtggatc agcaacagca ggggcagcgg ctgtggctgc
     2761 atctgaaaaa ccggccactc gataaagatt acaggcctca tcgtccgacc atcaacgaag
     2821 gcccggaagc ggggtattac tgggtggttg tccctgacgc ctccctcctg cctccgaaat
     2881 ggaaacagtg ggcgcgggtg acggtcgtgg ggcgtgtaat ggatcagagg gagacgaggc
     2941 cgacaacggt gccgacgagt gagccggtgt tgagcctcgt atttatgcgc gggtgggcca
     3001 tgggaaatgc ccagggaaaa ggcgcctggg aggaatcgg
//
LOCUS       DAOQEW010000328         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16121_length_3037_cov_8.065728, whole genome shotgun sequence.
ACCESSION   DAOQEW010000328 DAOQEW010000000
VERSION     DAOQEW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16121_length_3037_cov_8.065728"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLS35_19480"
     CDS             complement(<1..438)
                     /locus_tag="PLS35_19480"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNI20908.1"
                     /translation="MDAILRGFIMYLFLLVLFRIAGRRTLGQMTNFDFVLLLIISEAT
                     QNAMIGNDFSVTNGILVILSLVGLDIGFSILKQRFPILNRHLDGLPLVLVDQGRPVQE
                     LMRKTRVDEEDILFSAREKHGLERMEQIKYAVLETNGGISIIPK"
     gene            complement(450..941)
                     /locus_tag="PLS35_19485"
     CDS             complement(450..941)
                     /locus_tag="PLS35_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20909.1"
                     /translation="MAKDLDIQDDLPFQYRHWRLQRVGWWAIACILSAGLLGLFGHHP
                     LSRTTVQTQDGTLLVTYDRFGRATSDAEVLITVTPAKQEEGAFRVWFDSEYLDAVRVG
                     SVSPLPLRGEARQGGRAFVIQSDGGPSTVLFSVQFHTFGIIRGHVKINDGKAVPITHL
                     VWP"
     gene            1163..2056
                     /gene="ligD"
                     /locus_tag="PLS35_19490"
     CDS             1163..2056
                     /gene="ligD"
                     /locus_tag="PLS35_19490"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNI20910.1"
                     /translation="MKNHPGEALILHGVTITHPDRVVYKEERICKGDVARYYAAVAPF
                     LLRDITRRPISLLRCPSGIQADCFYQRNVGSGLGGHVHPFAWDYKGQSYQYIYVTDEM
                     GLMQMIQMGVIEIHPWGATIDSIHTPDRMIFDLDPDPTVPFSAVKQAATEIRQRLTKS
                     GLTSLLKCTGGKGLHVVVPLSPTRRWDEVKGWASTLAHAMVEEAPDRFVATMTKSKRA
                     GKIFIDYFRNDYTATSIAAYSLRARPGAPVAVPLQWRDLGTLKSADQFGMTAVLKRIA
                     RQQRPKPLPIQQLPVLKTERF"
     gene            2061..>3037
                     /locus_tag="PLS35_19495"
     CDS             2061..>3037
                     /locus_tag="PLS35_19495"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNI20911.1"
                     /translation="MAAPTFAPMEALLVSRLPEGHEWRYEPKWDGFRCLAYKRKTSVT
                     LHSKSGKPLDRYFPDIVRVLAKLKADNFVLDGELLIKGEEGYSFTDLQMRLHPAASRV
                     SMLAKKQPATFVLFDILEAETDSAVMSAPFAARRLRLESFYARYCAKEPALQLSPQTA
                     SIAEAMAWLKSTGWYIDGIVAKHTLAAYEPGERVMQKYKPIRTADCVVGGFRYGSGSE
                     EVGSLLLGLYDKNGRLNHVGFTSGIAKADKPSLTKTLEALVHEPGFDGKAPGGPSRWS
                     TERSTTWTPLSPQIVVEVSFDHVTDDRFRHGTKLLRFRPDKAPRQCTME"
BASE COUNT          706 a          889 c          833 g          609 t
ORIGIN      
        1 tttgggaatg atcgagattc caccattggt ttcaagcacg gcatatttga tctgttccat
       61 gcgctcgagg ccatgtttct cgcgggccga gaacagaatg tcctcttcgt ccaccctggt
      121 cttgcgcatc aattcctgca cgggccgccc ctggtccacc aacaccagcg gcagtccatc
      181 cagatgacga ttcaagatcg ggaatcgctg cttcaggatt gaaaagccaa tgtccaaacc
      241 cacgagggac aagatgacca gaataccgtt ggtcaccgag aaatcattcc cgatcatggc
      301 attttgggtc gcttcactga tgatcaagag cagcacgaaa tcgaaattgg tcatttggcc
      361 cagcgttcgc cgccctgcga tccggaacaa caccagcagg aacaggtaca ttatgaatcc
      421 ccgcagaatc gcatccatac atcctccctc tacggccaca ccaggtgcgt gatcgggacc
      481 gccttaccat cgttgatttt gacgtggcct cgaatgatcc cgaacgtgtg aaactgaaca
      541 gagaacaaca ccgtcgaggg gccgccatcc gactgtatga cgaacgcgcg accaccctgg
      601 cgcgcctcac ctcggagggg cagcggtgac acggacccca cgcgtacggc atccaggtat
      661 tccgagtcga accagacccg aaacgctccc tcctcctgct ttgcgggcgt cacggtaatg
      721 aggacttcag cgtcactggt ggcgcgtccg aaacgatcat aggtgaccag gagcgtgcca
      781 tcttgtgtct gcaccgttgt tcgagaaaga ggatggtgcc cgaacaaccc gaggagaccg
      841 gcagaaagga tacaggcaat ggcccaccat cccacccgct gcagtctcca atgacggtac
      901 tgaaatggga ggtcgtcctg gatgtctaaa tctttggcca taaggctttc tgccgatcat
      961 gggccatcct tttccgaatg aaacttaggt gaatccctag cggcgacagg ccgcagcttc
     1021 agtcactggg gcttttgcca ggacaaaacc cacatcccgc atgtcctcat agatcaagat
     1081 gctgccgtct cggcaggctc gattccctat acgcagaaca accgccacgg tcgatgactg
     1141 gcacagcggg actgccaagc cgatgaagaa ccacccaggc gaagccctta ttctgcatgg
     1201 agtgaccatc acgcatcctg atcgcgtggt gtacaaggag gaacggattt gcaagggcga
     1261 tgtcgctcgc tactatgcag ccgtcgcgcc gtttctcttg cgagacatca cccggcgtcc
     1321 cattagtttg ttgagatgcc cgagcggtat ccaggctgat tgtttttatc agcgcaatgt
     1381 cggctctgga ttaggcgggc atgtgcatcc atttgcgtgg gactataaag ggcagagcta
     1441 ccaatacatc tatgtgaccg acgaaatggg tctcatgcag atgattcaga tgggcgtgat
     1501 cgagatccac ccatggggcg cgacgatcga cagcatccat acgccagacc ggatgatttt
     1561 cgatctcgat cccgatccga ccgttccttt ctcagcggtc aaacaagcgg ccacggaaat
     1621 ccgccagcgc ttaacaaagt caggactcac gagcttgctg aagtgcacgg gcggcaaagg
     1681 cctgcatgtc gtggtgccgc tgtcgcccac cagacgttgg gacgaagtca aaggctgggc
     1741 ctcgacgttg gcgcatgcca tggtcgaaga ggcgccagac agatttgtcg ccaccatgac
     1801 caaatccaaa cgggcaggga agatattcat cgactacttt cgtaatgact atacggccac
     1861 gagcattgcc gcctattcgc tacgggcccg acccggcgca ccggtggccg tgccgttgca
     1921 gtggcgcgat ctcggcacgc tcaagagcgc tgatcaattc ggtatgacgg cagtgctgaa
     1981 acggatcgcc cggcaacaac gaccgaaacc cctgccgatc cagcagctgc cggtgttgaa
     2041 gacagaaagg ttctgaacgc gtggctgcac ctacattcgc tccgatggaa gccctgctgg
     2101 tcagccgttt gccggagggt cacgaatggc gatatgagcc gaaatgggat gggtttcgct
     2161 gtcttgccta taagcgtaag acgtccgtaa cactgcactc gaaatcgggc aaaccgttgg
     2221 acagatattt ccccgacatc gtgcgcgttc ttgccaagct gaaggccgat aatttcgtcc
     2281 tcgacgggga actgctgatc aaaggcgagg agggctattc atttaccgac ctccagatgc
     2341 gactgcatcc cgctgcgagc cgagtgagca tgctggcaaa aaaacagccg gcgacatttg
     2401 tccttttcga catactggaa gcagaaacag actcggccgt catgtcagca ccttttgccg
     2461 cacgccgcct tcggctcgaa tctttttacg cgagatactg cgcgaaagaa ccggccctcc
     2521 aattatcccc gcagacagcg tcgatcgctg aagccatggc ctggctcaaa tcgaccggtt
     2581 ggtatatcga tggcattgtc gccaaacaca ccctggccgc gtatgagccg ggcgagcgtg
     2641 tcatgcagaa atataagccg attcgcacgg cggactgcgt cgtgggagga ttccggtatg
     2701 gctcaggttc agaggaggtc ggatcacttc ttctgggact gtatgataag aacgggcgcc
     2761 tgaatcacgt cggctttacg tcgggaattg cgaaagcaga caagccttcg ctgaccaaga
     2821 cactcgaagc gctcgttcat gagccaggct ttgacgggaa ggctcccggt gggcccagcc
     2881 ggtggtcaac cgaacgatca acaacatgga cgccgctctc gccccaaatc gtcgtcgagg
     2941 tgagtttcga ccacgtcaca gacgaccgct tccgccatgg cacaaaactc ctccggtttc
     3001 gtccggacaa agcacctcgc caatgcacga tggaaca
//
LOCUS       DAOQEW010000329         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16255_length_3019_cov_10.307355, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000329 DAOQEW010000000
VERSION     DAOQEW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16255_length_3019_cov_10.307355"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="sthA"
                     /locus_tag="PLS35_19500"
     CDS             <1..962
                     /gene="sthA"
                     /locus_tag="PLS35_19500"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNI20912.1"
                     /translation="RPAHIPFDDQTIIDTDGLLKLKDLPKSITIIGGGVIGAEYASIL
                     ATMGLHVTLIERRPRLLEFVDQETIEALQYHMRSIGVVLRFNEEVVSVEPISKELVIV
                     RLKSGKEIAASTVLYSVGRTGASATLNLEAIGLAADERGRLTVNEHYQTSIPHIYAAG
                     DIIGFPALASTSMQQGRHAACHAFGVPYQTQSDLMPYGIYSIPEISMVGRNEDDLTKH
                     GVPYAVGIARYREIARGQIIGDEIGMLKLLFHNKTRQLLGVHAIGDGATELIHIGQTV
                     MAFQGQIDYFIDAVFNYPTLAECYRVAALDGINQLPRPWTPRA"
     gene            1074..1742
                     /gene="tenA"
                     /locus_tag="PLS35_19505"
     CDS             1074..1742
                     /gene="tenA"
                     /locus_tag="PLS35_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HNI20913.1"
                     /translation="MSFSNHLRKLAQPVWDAQLTHPFVLALGKGTLSERKFRYYILQD
                     ARFLADLARVFAAGSLRAPDSESALRFAKLAEETITVERSLHENYGKRWNLSPQEMLN
                     EPMAPTNYAYTRHMLAVAQAGTATEIAVVALPCAWIYCVVGKHFLKNGAPPAKHPYRD
                     WLMLYASPEFEAVQEWMRARVDTWARTAGHEEKKRMEQAFLISSKYEWMFWEMAWNEE
                     KWPV"
     gene            2084..2812
                     /locus_tag="PLS35_19510"
     CDS             2084..2812
                     /locus_tag="PLS35_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20914.1"
                     /translation="MPPHEEGKRKISFPNSNVSGGNIVVVLIENGKSPDQPYNARLGF
                     WLPPDSPYGNFQLKLRKLCERLDEANRRLIDTFDYWNQAMEQKTSVNLFQRHEYAIEQ
                     AVYLMRRVADELIAMIWCLHEWEKTGDYPRQIRIDCIGAALESTQQNLSKDLKVLFDP
                     HNPVMKALNEISNAFKHSFVHSDLTLIGRDEPCVYALGLAHNKLSSEMKFHNFSVSWL
                     VREFSAFYKSGMQWLASFSERHRK"
BASE COUNT          733 a          866 c          770 g          650 t
ORIGIN      
        1 cccgtcccgc ccacattccc ttcgacgatc agacgatcat cgataccgac ggcctcctga
       61 agctgaagga cctccccaag tccatcacca tcatcggcgg cggagtgatc ggcgcagaat
      121 acgcttccat cctggccacg atgggcctcc acgtcacgct catcgagcgc cggccgcgcc
      181 tgctggaatt tgtcgaccag gaaaccattg aagcgctgca gtaccatatg cgcagtatcg
      241 gagtcgtcct tcgattcaac gaagaggtcg tgtctgtcga gccgatctcc aaagaactag
      301 tcatcgtccg cttaaagagc gggaaagaaa tcgccgcctc caccgtgctg tattcggtcg
      361 gccgtaccgg cgccagcgcg acgcttaatc tcgaggccat cggactcgca gcagatgagc
      421 gcgggcgcct cacggtcaac gagcattatc aaacatccat ccctcatatt tatgcggccg
      481 gtgacatcat cggatttccc gcgctcgcct ccacgtccat gcagcaaggc cgtcacgccg
      541 cttgccatgc gtttggcgtg ccctaccaga cgcaatcgga cctgatgccc tatggcatct
      601 attcgatacc cgagatttcg atggtcggcc gcaacgaaga cgacctgacc aaacacggag
      661 tcccctatgc cgtcggcatc gcccgctacc gcgaaatcgc ccgcggccag atcatcggtg
      721 atgagatcgg catgttgaag ctgctctttc acaataagac ccgtcaattg ctcggcgtgc
      781 atgcaatcgg cgacggtgcg acggaactga ttcacatcgg gcagacggtg atggcctttc
      841 aaggacagat cgattacttc atcgatgcgg tgttcaatta ccccaccctc gcggaatgtt
      901 atcgcgtcgc cgcgctggat ggcatcaatc aactcccgag accctggaca ccgcgagcct
      961 gacaggcccc tgtctcgcga gccgcaatag tcctcaatgc cccatgtcag gcattgaact
     1021 gaggagtgcc catcgagaat tcaatagtct ccgtaagagc aaggaggacc cggatgtcgt
     1081 tttcaaatca tcttcgtaag ctggcccaac ccgtgtggga tgcacaactg acgcacccct
     1141 tcgtcctcgc attaggcaag ggcaccttgt cggaaagaaa attccgctat tacatcctgc
     1201 aggacgcccg gtttctcgca gaccttgctc gggtctttgc cgccgggtcc ttacgagctc
     1261 cggactccga atcggcgctt cgcttcgcca agctggcaga agaaaccatc accgtcgaac
     1321 gcagtctgca tgaaaactac ggcaaacgct ggaacctttc gccgcaagag atgctgaacg
     1381 agccgatggc gccgaccaac tatgcctaca cccgccacat gctggccgtg gcccaagcgg
     1441 gtacggcgac ggaaatcgcc gtcgtggcgc tcccctgcgc gtggatttat tgtgtcgtcg
     1501 gcaagcattt tctcaagaac ggagcgccgc cggcaaaaca cccgtaccgc gattggctga
     1561 tgctctatgc gtcaccggag ttcgaggcag ttcaggaatg gatgcgcgcc agggtcgata
     1621 cctgggcgcg aacagccggg catgaagaaa aaaaacggat ggagcaggcg tttctgatca
     1681 gttcgaagta tgagtggatg ttttgggaaa tggcctggaa tgaggagaag tggccggtgt
     1741 gagggggcgg gcagcgatgg cggcactcta cacgccggaa acaaagccgc agcagcgttc
     1801 ggcagtgagg tgatcctttc cgcctgaact tgctctgttc gtgctcgcac gaacagagct
     1861 tcgaacaaca ggcgtcacct gcaacgcagg acggttcacc ccaccgtcga acgaggtcct
     1921 gctgacgctt tgagggcgtt ccgagtgtcg ccatcgctgc ccgccagtca cacgtccact
     1981 gaaaggtagc tgaaataaca atacttccag catagcggaa ccctaattta tcggctttct
     2041 actgtgtact tacgcgacag gagcttgaag gaataactag actatgccac ctcatgagga
     2101 aggaaaaaga aaaatcagtt tccccaattc gaacgtaagc gggggaaata tcgtggtggt
     2161 tctgatcgaa aatgggaaga gcccagatca accctacaat gcccgtcttg gattctggtt
     2221 gcctccagat tctccctatg gcaactttca actgaaactt agaaagctgt gcgagagact
     2281 cgacgaagct aatcgtagat tgatcgacac tttcgattat tggaatcagg caatggagca
     2341 gaaaacttca gtcaacttgt tccaacgtca cgagtatgca atcgaacagg ctgtctatct
     2401 catgagacga gtggctgacg aattgatagc aatgatctgg tgtctacatg agtgggaaaa
     2461 aacgggggac tatccgcgcc aaattaggat tgattgtatc ggtgccgcgc ttgaatctac
     2521 gcaacaaaac ctttcaaaag accttaaagt gctttttgac cctcacaatc cagtcatgaa
     2581 agctctcaac gaaatttcaa atgcattcaa acactccttc gttcactcag atttaacatt
     2641 gattggaagg gacgagccct gcgtttatgc tctcgggtta gcccataata aactttcttc
     2701 tgaaatgaaa ttccataatt tctcggtgtc ttggttagta cgcgaattca gtgcctttta
     2761 taaatccggg atgcaatggc tcgcatcatt ttccgaaaga catcgaaaat gaattcagcc
     2821 ttcaatattc actgttccct atgcacgcgc ataggattcc acccaaacgg cagcattctc
     2881 acttctcaat aaaggggtcg ccccccactc ggaatatgtc cctaagagaa tcgccgctcc
     2941 gattgatctc tcgttcaggc acgacactgc aggctacaca gcccctctct gaccgttagc
     3001 atgggacaca ccgtacagt
//
LOCUS       DAOQEW010000330         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16314_length_3013_cov_6.168357, whole genome shotgun sequence.
ACCESSION   DAOQEW010000330 DAOQEW010000000
VERSION     DAOQEW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16314_length_3013_cov_6.168357"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2871
                     /locus_tag="PLS35_19515"
     CDS             <1..2871
                     /locus_tag="PLS35_19515"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNI20915.1"
                     /translation="LLDNIQQVQRGQNLPVSDTLVKNKVARVNLDVEMETGTGKTYCY
                     IKTIFELNRRYGWSKFIIVVPSIAIREGVAKSLEITAEHFLETYQKKARFFIYNSKQL
                     HHLESFSSDAGINVMVINVQAFAARGADNRRIYEVLDDFQSRRPIDVISANRPILILD
                     EPQKMEGAQTLKSLEEFKALMVLRYSATHKTTHNKIHRLDALDAYNQKLVKKIAVRGI
                     AVKGLAGTAGYLYLQSIEISSKKPPEARVEFEQKLAGGNIKRVVKKLSKGDNLFSDGF
                     SNGLDQYRDGFVVSDINANTDTLSFTNGLQLTVGDATGDVTEAALRRIQIREAIKAHF
                     DKEQALFQQGVKVLTLYFIDEVAKYRDYLQPDEKGEYARIFEEEYNLYLNEMLDLDET
                     PYVKYLKGITAEKTHSGYFSIDKKTKRDVDPSSAKTGENKGLSDDVDAYDLILKKKEQ
                     LLSLEEPVRFIFSHSALREGWDNPNVFVICFLKHPDYNNEVTRRQEVGRGLRLSVNKY
                     GDRMDHPSIVHDVNVLTVVASESYKDFVAALQKDISDSLSARPKVADEAFFTGKVLKT
                     ATGDVEVTPQLAKQIYKYLLKNDYTDDNDRITGTYHDAKKAEALADLPPELKPYAVQV
                     YQLIDSVFSASQLPEIGDDRRPKKNPLNANFDKQEFKALWSRINRKAAYSVSFEEAEL
                     VQKAVAVLNDKDAGLRVTPLQYTIQRGEQAEAVTYDGIKSGEAFKLKATETEVNRLSV
                     HSAVKYDLIGKLAEGTQLTRRTIAEILKGMNVAVFAQFKTNPESFIAEAVRLINEQKA
                     TVIIEHLAYDPVEDKFDLDIFTAGLTKQDFSKAGDKLQRHICDYVLTDSNIERQFVKE
                     LDTSCDVVVYAKLPRGFLIPTPVGDYNPDWAISFKEGAVKHVYFVAETKGSMSSMELR
                     EIEKTKIKCARRFFDDINQKFAPEQVKYDVVDSFGKLMELVK"
BASE COUNT          718 a          918 c          856 g          521 t
ORIGIN      
        1 ttgctggaca acatccagca ggtgcagcgc ggccagaacc tgcccgtgtc tgacacgctg
       61 gtgaagaaca aggtcgcccg cgtcaacctc gacgtggaga tggaaacggg cacgggcaag
      121 acctactgct acatcaagac gatcttcgag ctgaaccgcc gctatggctg gagcaagttc
      181 atcatcgtgg tgcccagcat cgccatccgt gagggcgtgg ccaagtcgct ggagatcacc
      241 gccgagcact tcctggagac ttaccagaag aaggcgcgct tcttcatcta caactccaag
      301 cagctgcacc acctggagag cttttcgtcg gatgcgggca tcaacgtcat ggtcatcaac
      361 gtgcaggcct ttgccgcccg tggtgccgac aaccgccgca tctacgaagt gctggacgac
      421 ttccagtcgc gccgcccaat tgatgtcatc agcgcgaacc ggcccatcct gatcctggac
      481 gagccgcaga agatggaagg cgcgcagacc ttgaagtcct tggaggagtt caaggccttg
      541 atggtgctgc gctactcggc cacgcacaag acaacgcaca acaagattca ccgcttggac
      601 gcgctggacg cctacaacca gaagctggtg aagaagattg ccgtgcgcgg catcgccgtg
      661 aagggcctgg cgggcactgc gggctacctg tacctgcaat ccatcgagat ttcgagcaag
      721 aagccgccag aggcccgcgt cgagttcgag cagaagctgg cgggcggcaa catcaagcgc
      781 gtggtcaaga agctgtccaa gggcgacaac ctcttctcag atggcttttc taacgggctg
      841 gaccagtacc gcgacggctt cgtggtgtcg gacatcaatg ccaacactga caccctgagc
      901 ttcaccaacg ggctgcagtt gacggtgggc gatgccactg gcgatgtgac cgaggccgca
      961 ctgcgccgca tccaaattcg cgaggcgatc aaggcccact ttgataagga acaagccctg
     1021 ttccagcaag gcgtgaaggt cctgacgctg tacttcatcg acgaggtggc caagtaccgc
     1081 gactacttgc agcctgacga gaagggcgag tacgcccgca tcttcgaaga ggaatacaac
     1141 ctctacctca acgaaatgct ggacctggac gagacgccct acgttaagta cctcaagggc
     1201 atcacggccg agaagaccca cagtggctac ttctccatcg acaagaagac caagcgcgac
     1261 gttgacccga gcagtgcgaa gactggcgaa aacaaaggcc tgtcggacga tgtggacgcc
     1321 tatgacttga tcctgaagaa gaaagagcaa ctgctcagtc ttgaagagcc tgttcgtttc
     1381 atcttttcgc actcggccct gcgggaaggt tgggacaacc ccaacgtgtt cgtcatttgc
     1441 ttcctgaagc accccgatta caacaacgag gtcacacgtc gccaagaggt cggacgcgga
     1501 ctgcgcctgt cagtgaacaa gtatggcgac cgcatggatc acccctcaat cgtccacgac
     1561 gtgaacgtgc tcaccgtggt ggccagcgag agctacaaag acttcgttgc agcgctgcaa
     1621 aaggacatca gtgactcgct gtctgctcgt ccgaaggtgg ccgatgaagc cttcttcacc
     1681 ggcaaggtgt tgaagacggc aacgggcgac gtggaagtca cgccgcagtt ggcgaagcag
     1741 atctacaagt acctgctcaa gaacgactac accgacgaca acgaccgaat caccggtacg
     1801 tatcacgacg cgaagaaggc tgaagccttg gccgatctgc cgcctgaact gaagccctat
     1861 gcggtccagg tctatcagtt gatcgacagc gtgttcagtg ccagccagtt gcccgagatc
     1921 ggtgacgacc gtcgccccaa gaagaacccg ctcaacgcca acttcgacaa gcaggagttc
     1981 aaagccctgt ggagccgcat caaccgcaaa gcggcctaca gcgtcagttt cgaagaggcc
     2041 gagctggtcc agaaggccgt ggccgttctc aacgacaaag acgcgggcct tcgcgtcacg
     2101 ccgttgcagt acacgatcca gcgcggcgag caggccgagg ccgtcaccta cgacggcatc
     2161 aagtccggcg aagcgttcaa gctcaaggcc acggaaaccg aggtcaaccg tttgtcggtg
     2221 cactcggccg tcaagtacga cctcatcggc aaactggccg aaggcacgca gctcacccgc
     2281 cgcacgattg ctgaaatctt gaagggcatg aacgtcgcgg tcttcgcaca gttcaagacc
     2341 aaccccgaga gcttcatcgc cgaagcagtc cgcttgatta acgagcagaa ggccacggtc
     2401 atcatcgagc acttggccta tgacccggtg gaagacaagt tcgacctcga catcttcacg
     2461 gccggcctga ccaagcagga cttcagcaag gccggtgaca agctgcaacg ccacatctgc
     2521 gactatgtgc tgaccgactc caacattgaa cgccagttcg tgaaggagct ggacaccagc
     2581 tgcgatgtcg tggtctacgc caagctgccc cgtggcttcc tgatccccac gccagtcggc
     2641 gactacaacc ccgactgggc catctcattc aaggaaggcg cggtcaaaca cgtctacttc
     2701 gtggcggaga ccaagggctc catgtccagc atggagttgc gcgagatcga gaaaaccaag
     2761 atcaagtgcg cccgcaggtt cttcgacgac atcaatcaga agttcgcccc ggagcaggtc
     2821 aaatacgacg tggtggacag cttcggcaag ttgatggaac tggtgaagta acgaggcttc
     2881 aggagtacgg tcgcatgatt cagagttcaa accgtaacat tcccttcata taatcccgcc
     2941 gataatcccc cgctgcgccc gctgcgtgcc ctccgatgcg cgcgctgcag gccggcgcac
     3001 agccgacccg tcc
//
LOCUS       DAOQEW010000331         3011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16326_length_3011_cov_7.769621, whole genome shotgun sequence.
ACCESSION   DAOQEW010000331 DAOQEW010000000
VERSION     DAOQEW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16326_length_3011_cov_7.769621"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..789
                     /locus_tag="PLS35_19520"
     CDS             106..789
                     /locus_tag="PLS35_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNI20916.1"
                     /translation="MSRPSPEIAHLSTIDPVMRRVIDAVGPYALPPPPRRSPFEALVR
                     AIAYQQLHEKAAECILTRFIALCPGRRFPQPADVLALAPESMREAGFSRAKIAALQDL
                     AAKAIDGTLPTAAVIRRLDDDAIVDRLVAVRGIGRWTVEMLLIFQLGRPDVLPVDDFG
                     VRNGFRIAYKRPMPTPQELLRYGERWRPYRTAAAWYLWRAVDQAKALQAVTPLSKPST
                     RRVSNGRSA"
     gene            773..1651
                     /locus_tag="PLS35_19525"
     CDS             773..1651
                     /locus_tag="PLS35_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNI20917.1"
                     /translation="MADPHKRLDANIPGNFYVDDTCINCDACRQLAPASFEEVGDYSA
                     VRHQPASAQEIREAYRALLACPTGSIGTEQGDKGVMQAAKADFPLLVEDHVYYCGFNS
                     EKSFGANSFFIRHPEGNWLIDSPRYLKPLVEAFDRMGGIAHIFLTHEDDVADAARYAQ
                     RFGGRRIIHRADAHVMPEAEEIVEGLDAMFYRPEFLIIPVPGHTAGSLCLLYRDRFLF
                     TGDHLWWDPETNELGSPQQLVWNADALRRSIATLTSYPFEWVLAGHGGRIHLPHAHMQ
                     MALRALVRRREGTRLS"
     gene            1648..2325
                     /locus_tag="PLS35_19530"
     CDS             1648..2325
                     /locus_tag="PLS35_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNI20918.1"
                     /translation="MKVMLASMCAGLVMVVLGMAVTASPAPDKDQLLIAAAERNDVES
                     VKRLLRDGAGVQARDAQGRTGLMAATYHNHVDVARVLIEAGSDVNAQDAMHNSPLLLA
                     GASGYLDIVRMTLAARPDFTVYNRFGGTALIPACERGHVEVVKALLKTTIDVNHVNRL
                     GWTALLEAILLSDGGTAHQEIVRLLLAAGANPNLADHDGVTPLRHARQKGYRVISDLL
                     LAAGAHE"
     gene            complement(2344..2697)
                     /gene="uraH"
                     /locus_tag="PLS35_19535"
     CDS             complement(2344..2697)
                     /gene="uraH"
                     /locus_tag="PLS35_19535"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HNI20919.1"
                     /translation="MEDAHRISIHVLDQSQGRPAEGIPVTLEIRGLTGLWKQLGQGRT
                     DADGRSSDLYPAGLRMQTGTYRLTFDVAGYFRKRNVATFFPEVPIVFTVQDVAQSYHI
                     PLLLSPFGYSTYRGS"
     gene            complement(2721..>3011)
                     /locus_tag="PLS35_19540"
     CDS             complement(2721..>3011)
                     /locus_tag="PLS35_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNI20920.1"
                     /translation="PELSRSDAEGLQGNSLETIRQMVASGLGITVMPCSAVTSRYLNK
                     RLTVLEFAKPVPERRIALAWRKGFARPAVIAVIHEAVGMLKIPGLESISAQA"
BASE COUNT          567 a          903 c          925 g          616 t
ORIGIN      
        1 tcaggcctgc gaccaccatg gtggaggtga agcgcttgat cgatccggac atgctcgtcg
       61 aaatcgaagc cgacgccatc gtcgcctagc agcagtggga gcaccatgag caggccgtcg
      121 cctgaaattg cgcatctgtc tacgatcgat ccggtgatga ggcgggtgat cgacgcggtc
      181 ggcccctatg cgctgcctcc tccgccaagg cgctctccat ttgaagcgct ggtccgagcc
      241 atcgcctacc agcaacttca tgagaaggcg gcggagtgca ttcttacccg attcatcgcg
      301 ttgtgcccgg gccgccggtt tccacagccg gccgacgtgt tggccttggc cccggagagc
      361 atgcgagaag cgggattctc gcgcgcgaag attgccgcgc tgcaggattt ggcggcgaag
      421 gcgatcgatg gcaccctccc taccgccgct gtcattcgac ggctcgatga cgacgccatt
      481 gtcgacaggc tggtggcggt gcgagggatc ggccggtgga ccgtcgagat gttgctgatt
      541 tttcaactgg gccgtcccga tgtcctgccg gttgatgact tcggcgtgcg gaacggcttc
      601 cggatcgcct acaagcggcc gatgccgacc cctcaagaac tgttacgata tggagaacgg
      661 tggcggccgt atcggactgc cgccgcctgg tatctgtggc gcgcggtcga tcaggcaaaa
      721 gccctgcaag ccgtgacgcc gctgtcgaaa ccttcgacca ggcgagtctc caatggccga
      781 tccgcataag cgattagacg ccaatatccc cggcaacttt tatgtcgatg acacctgcat
      841 caattgcgat gcctgtcgtc aacttgcccc tgccagcttt gaggaagtcg gagactactc
      901 agccgtgcgg catcaaccgg ctagtgcgca ggaaatcagg gaggcctacc gagcgctcct
      961 cgcctgcccc accggatcga tcggaaccga gcagggcgac aagggcgtga tgcaggcagc
     1021 gaaggccgac ttccctctcc tcgtggaaga tcacgtctac tattgcggct tcaattcaga
     1081 aaaatccttc ggcgcgaaca gcttcttcat ccggcatccg gagggcaact ggctgatcga
     1141 ttcgccgcga tatctcaaac ctctggtcga ggccttcgat cggatgggag gaatcgcaca
     1201 catctttctg acccatgaag acgatgtggc tgatgccgcc aggtacgcgc agcgattcgg
     1261 ggggagacgc atcattcacc gggcggatgc gcatgtgatg ccggaagcgg aagagatcgt
     1321 ggaggggctc gatgcgatgt tctatcgacc ggaatttctg atcatcccgg tccccggcca
     1381 tacggcgggc agcctttgtc tcctatatcg ggaccggttc ttgttcacgg gtgatcatct
     1441 ctggtgggat ccggagacca atgaattggg ctcccctcag caattagtct ggaacgccga
     1501 tgcattgcgc cgatcgatcg cgacgttgac gtcctatcca ttcgaatggg tgctggccgg
     1561 tcatggagga cggattcatt tgcctcacgc gcacatgcag atggccttac gcgcattggt
     1621 ccgtcgccgg gagggaacgc ggctatcatg aaggtcatgc ttgcttcgat gtgtgccggt
     1681 ctcgtcatgg tcgtgctagg catggccgtc accgcctccc ctgctcccga caaggaccag
     1741 ctcttgatcg cagcggcgga acgcaatgac gttgaatcag tgaaacggtt gttgcgagat
     1801 ggcgccgggg ttcaggctcg tgatgcgcag ggtcgcacgg gactcatggc cgcgacgtat
     1861 cacaaccatg tcgacgtggc gagggtgctg atcgaagccg gttcggacgt gaatgcgcag
     1921 gacgccatgc acaacagccc cttgctgctg gccggggcca gtggttatct ggatattgtg
     1981 cgaatgacat tggccgcgcg cccggacttc acggtctaca atcgtttcgg tggtacggcg
     2041 ttgatcccgg cctgtgagcg cggccatgtc gaggtggtca aggccttgct caagaccacg
     2101 atcgatgtga atcacgtgaa ccgcctcggc tggacggccc tgcttgaggc catcctattg
     2161 agtgacggcg gcacggcgca tcaagagatc gttcgtctgc tcctcgctgc cggagcgaat
     2221 ccgaatcttg ccgatcacga tggtgtcaca ccgctgcggc atgccaggca gaagggttac
     2281 cgcgtgatct cagatctgct gctggctgcc ggtgcgcacg agtagacgga ctcgcgtgtt
     2341 cgctcaactg ccacgatagg tgctatagcc gaagggactg agtagcagtg ggatatggta
     2401 gctctgcgcc acgtcctgca cggtaaacac gatcggcacc tccgggaaga acgtcgccac
     2461 gtttcttttc cgaaaatagc cggccacgtc gaatgtgagc cggtaggtgc cggtttgcat
     2521 gcggaggccc gcgggataga ggtccgagct gcggccgtcc gcatcggttc gtccttggcc
     2581 gagctgcttc cagagaccgg taagccctcg aatctccaac gtcaccggga ttccttctgc
     2641 cggccgtcct tgcgattgat ccaacacatg gatgctgatc cgatgtgcgt cctccataaa
     2701 cgctcctttc acacgatccg tcaggcctgt gcggagatcg attccagccc cgggatcttt
     2761 aacatcccca cggcttcatg aatcaccgcg atgacggccg gtctggcaaa tcccttgcgc
     2821 cacgccaggg caatgcgccg ctcgggcacc ggtttcgcga attccaacac ggtgagccgt
     2881 ttgttcaaat atctgctggt cacagcgctg caaggcatga ccgtaatgcc cagtccggag
     2941 gccaccatct ggcgaatcgt ctccagcgaa ttgccctgca agccttccgc atctgagcgg
     3001 ctgagctccg g
//
LOCUS       DAOQEW010000332         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16431_length_3000_cov_8.678438, whole genome shotgun sequence.
ACCESSION   DAOQEW010000332 DAOQEW010000000
VERSION     DAOQEW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16431_length_3000_cov_8.678438"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLS35_19545"
     CDS             <1..304
                     /locus_tag="PLS35_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20921.1"
                     /translation="VCSSQYCLWGSSPQHKALFSKSPLSCGDRSILDAVFARESQQPL
                     EKIMKLNPYLVRLFDPLPMAFACDLYQRLREGKRDMETRDMLLKALDMARKAGTIE"
     gene            504..1337
                     /locus_tag="PLS35_19550"
     CDS             504..1337
                     /locus_tag="PLS35_19550"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HNI20922.1"
                     /translation="MKLNHPGLLVMPATQYHAEKAIGHSGIVKMLKSPAHLREYLDHP
                     HQPTPAMAFGTAVHTYVLEQDRFAEEFVVAEKFDRRTKEGKEAAARFEEANQGKIVIT
                     SEEMATLTLVQRAVFAHQGAARLLSVGDAELSAFWTDADTGLNCKCRPDWFNGEAIVD
                     LKTCVDASSSGFSKSIANFGYDIQAAYYVDGIKAVTGMELPFLFVAVEKDAPHAVAVY
                     RADPDVIEIGRKKYRAALQLLKWCQASGNWPAYQPGGEIELISLPRWAANTEAFELDA
                     A"
     gene            1434..2531
                     /locus_tag="PLS35_19555"
     CDS             1434..2531
                     /locus_tag="PLS35_19555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HNI20923.1"
                     /translation="MSQTLRNIQKSRQGVQSNVVPFPAMLEQFKGEIARALPKHLSPD
                     RMVRIALTAFRMNPKLAQTDPRSVFAAVIQSSQLGLEVGLMGEAHLVPFGNQCQLIPG
                     YQGLMKLARNSGIVQDIYGHEVRINDKFDIVLGLNRTLMHEPLKKNGFPASDEERGMI
                     VGFYAVAVFKDGTRTFHALSKEQIEQVRDNSRGYQMAKKYGKESPWDTHFVPMGLKTV
                     IRRLCNLLPKSPELAMALALDEVTERGENQNIGITEAIEGSWAPIIDEETGEIIQPTT
                     ESGDKGSKPDKAESPKGAEPQAAGKLLQDTLAIIGRAATTDELDEVYVRAEGSFEGTE
                     LEQVLRAYRSRKSALANPSSSANDIFGEDGK"
     gene            2528..2740
                     /locus_tag="PLS35_19560"
     CDS             2528..2740
                     /locus_tag="PLS35_19560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20924.1"
                     /translation="MTMLGDTEFGAIRICARAVQVLDKVGFLTLSKEDDAAVVLARNE
                     LLSVIQGNGYQLEYDSYRLVKAGDHH"
     gene            2776..>3000
                     /locus_tag="PLS35_19565"
     CDS             2776..>3000
                     /locus_tag="PLS35_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20925.1"
                     /translation="MHKERDQDHRPDPARMTGHEANFDDVIPWTEDDILRLHGLLLEK
                     SLHDLFDLRVSAKTRADILDWMRAPRSESGA"
BASE COUNT          648 a          888 c          902 g          562 t
ORIGIN      
        1 ggtctgtagt tcgcagtatt gcctgtgggg gtcttccccg cagcacaaag cgcttttctc
       61 gaagagccct ttgtcctgcg gggatcgctc catcttggat gcggttttcg ccagggaatc
      121 tcaacaacct ctggagaaaa tcatgaaact caatccctat ctcgttcgac tgttcgaccc
      181 gctgcccatg gcctttgcct gcgatctcta tcaacggctg agggaaggca agcgggacat
      241 ggaaacccgc gacatgttgc tgaaggcact cgacatggcg cgcaaagccg gaaccatcga
      301 atgatcccat cgccccggac ttccggggct ttcccgaggg cgttcgcccc gagcgtcctg
      361 cggaaagaca gatttgatcg gacggttatc cgttcgattg aaaagcggta gctggccgcg
      421 cgaaacaaac agcattttcc aacccagccg gggacaacgc cccggttcgg gtcggtgctt
      481 ccggcattca ctctggaggc atcatgaaac tcaatcatcc cggcctgctc gtcatgccgg
      541 ccacgcagta tcacgcggag aaagccatcg gccattcggg tatcgtcaag atgctcaaaa
      601 gcccggccca cctgcgcgag tatctggatc atccgcatca accgacacct gccatggcgt
      661 tcggtactgc agtgcatacc tatgtactgg agcaggatcg ctttgccgag gaattcgtgg
      721 tggccgagaa gttcgaccgt cgcacgaagg aaggcaagga agcagcagca cgcttcgagg
      781 aagccaatca gggcaagatc gtgattactt ccgaggagat ggccacgctc accctggtgc
      841 agcgtgccgt gtttgcccat caaggggcgg cacgacttct gtccgttggt gacgccgagc
      901 tctcggcgtt ctggacggat gcggacacgg gcctcaactg caagtgtcgg ccggactggt
      961 tcaacggtga agccatcgtt gatctcaaga cctgcgtcga tgccagcagt agcggcttct
     1021 ccaaatccat tgcgaacttc ggctacgaca tccaggcggc gtactacgtc gacgggatca
     1081 aggccgtgac cggaatggag cttccgttcc tgtttgtcgc tgtggagaag gacgcgccgc
     1141 acgcggtagc tgtttatcgc gccgatcccg acgtgatcga gatcgggcgc aagaaatacc
     1201 gggcggcact tcagctcttg aagtggtgcc aggcatcagg caactggccg gcgtaccaac
     1261 cgggtggcga gattgaactg atctccctgc cacgctgggc cgccaacaca gaagccttcg
     1321 agctggacgc cgcctaatcc gcggcatttc tttgtaggtc atcaaccccg gcggggatac
     1381 ccatccccgt ccagggagac aggtgtcttc gccattcttc tttggagaaa accatgtctc
     1441 aaaccctacg caacatccag aagtcccgcc aaggcgtgca gtccaacgtg gtgccgttcc
     1501 cggccatgct ggagcagttc aaaggcgaaa ttgcccgcgc actgcccaag cacttgagtc
     1561 cggatcgcat ggtgcgcatc gcactgaccg cgttccgcat gaacccgaaa ctggcccaga
     1621 ccgatccccg cagcgtcttt gcggcggtga tccagtccag ccagctcggg ctcgaagtcg
     1681 gcctgatggg cgaagcgcac ctggtgccct tcggcaatca gtgccagttg atccccggct
     1741 atcagggtct catgaagctg gcccgcaaca gcggcatcgt gcaggacatc tacggtcacg
     1801 aagttcgcat caacgacaag ttcgacatcg tcctgggcct taaccggacc ctgatgcacg
     1861 aaccgttgaa gaagaacggc ttccctgcct ccgacgagga gcggggaatg atcgtgggct
     1921 tctatgccgt cgcggtgttc aaggacggca cccgcacgtt tcacgccctg tcgaaggaac
     1981 agatcgaaca ggtgcgcgac aactcgcggg gctatcagat ggccaagaaa tacggcaagg
     2041 aaagcccgtg ggatacgcat ttcgtcccga tgggcctgaa gaccgtgatt cgccggctgt
     2101 gcaacctgtt gccgaagtcg cctgaactgg cgatggcgct ggcgctcgat gaagtgaccg
     2161 agcgtggtga aaaccagaac atcggaatca ccgaagccat cgaaggttcc tgggcgccaa
     2221 tcatcgacga ggaaacgggg gagatcattc aacccacgac cgagtccggt gacaagggaa
     2281 gcaagcctga caaggcggag tcgcccaaag gcgcagagcc tcaagccgct ggcaagctgc
     2341 tgcaggacac cctggccatc atcggcaggg cggccacgac cgacgaactg gatgaagtct
     2401 atgtccgggc cgaaggttcg ttcgagggca ccgagctgga acaggtgctg cgcgcctacc
     2461 gtagccgtaa gtctgcactg gccaatccgt cttcctcggc gaatgacatt ttcggggagg
     2521 atggcaaatg accatgctcg gtgacacgga gttcggagcc atccggatct gtgcgcgggc
     2581 ggtgcaagtg ctcgacaagg tcggtttcct gaccctgagc aaggaagacg atgccgctgt
     2641 ggtactggcg cgtaacgaac ttctgagcgt gattcagggc aacggctatc agttggagta
     2701 cgacagctac cgcctggtca aggccggcga ccaccactag cgcaggcaat tcacgtgctg
     2761 atgacggaga tggccatgca caaggaacgc gatcaggacc atcggcccga tccagctcgc
     2821 atgacaggac atgaagcgaa ctttgatgac gtgattccat ggaccgagga tgacatcctg
     2881 cggctgcatg ggctccttct ggagaaatcg ctgcacgacc tgttcgatct gagggtgtcc
     2941 gccaaaacac gggcagacat tctcgattgg atgcgggcgc cacgttcgga gtccggtgct
//
LOCUS       DAOQEW010000333         2996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16469_length_2996_cov_6.586875, whole genome shotgun sequence.
ACCESSION   DAOQEW010000333 DAOQEW010000000
VERSION     DAOQEW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16469_length_2996_cov_6.586875"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /gene="gmk"
                     /locus_tag="PLS35_19570"
     CDS             <1..587
                     /gene="gmk"
                     /locus_tag="PLS35_19570"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNI20926.1"
                     /translation="LWHSVSYTTRKPRPGEVNGKDYHFLDETAFRQMIDRNEFVEWAH
                     VYGNLYGTPRKELTEKMEQGIDVLLEIDVQGARSVKKKFEDGVYIFILPPSFDTLRTR
                     LQNRAGDSPEEIQRRLQKAKEEVWSYREYYYIVRNDDLKQSLKELESIFLAERIKTKR
                     LNMTWLEEKFILDKEGKQGNSSSAPASKEHMHHG"
     gene            580..942
                     /gene="rpoZ"
                     /locus_tag="PLS35_19575"
     CDS             580..942
                     /gene="rpoZ"
                     /locus_tag="PLS35_19575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNI20927.1"
                     /translation="MGEMLSLLPEYATGEFDSRHRLVIVAAQRAKQLVQGARTTASSK
                     FTKETSIALDEVLRHNVKFLVGKEARDAIKESKRVKEGETERLAMMTGEDAKEIKKEL
                     SVYVDDTAKPVAAPEAEE"
     gene            952..2166
                     /gene="coaBC"
                     /locus_tag="PLS35_19580"
     CDS             952..2166
                     /gene="coaBC"
                     /locus_tag="PLS35_19580"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNI20928.1"
                     /translation="MQTTGPSLSGVRLVLAVTGSIAAYKAVSLLRLLRREAATVNVVM
                     TAGACRFVTPLTFEVLSGAHVATDLFEAHQEMLHLSLPEQAQAIVIAPATANCLAKAA
                     LGLADDLLSTMLLTTQCPVIFAPAMDGDMWQHPTVVEHVATLRARGAIIVEPEDGPLA
                     SGRQGQGRLADEERILAAVHHAVHPRRDWSGRKVLISAGPTQEAIDPVRFLSNRSSGK
                     MGYALAEAARARGADVVLVSGPTSIPVPAGVEYCPVITAEEMHKALTTQLDWSDTVIM
                     AAAVADFRPARPSAHKIKKRRGPVTHLDLEATDDILCELRDRRTSQVLVGFAAETDDL
                     LAHAKEKLHAKGLDLLVANDVTAPGSGFGSDTNRVWLLAREADPEELPLLSKRDVADR
                     ILDRILTVHTGR"
     gene            2209..2895
                     /locus_tag="PLS35_19585"
     CDS             2209..2895
                     /locus_tag="PLS35_19585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20929.1"
                     /translation="MSSPLRIPPAIAAEIDRLATAVAKDPRSKDFLPLADEYIKAGMW
                     QEAAAVLEDGLKVYPGFVTAMAALGRVYDQLGQAEKAKVILEEVVKQRPDNLRAHRIL
                     AKLYHAEGQADLALRSCTAILNANPFDEEALAVKRSVTGAPDEPPPSKREKKRSLAEA
                     PVEKITAAVPPAPAPVVLVSESAAPTVSMPDPTPPQSPPSPAVQHAAAIARLEAWLST
                     IQAKRHPAAR"
BASE COUNT          665 a          936 c          862 g          533 t
ORIGIN      
        1 gtctctggca ctcggtgtcc tacaccacgc gcaaaccgcg gccgggggaa gtgaacggca
       61 aggactatca ttttctggac gagacggcat tccggcagat gatcgatcgg aacgaattcg
      121 tggaatgggc ccatgtctac ggaaatctct acggaacgcc tcgcaaagag ctgacggaaa
      181 aaatggagca gggcatcgac gtcctgctcg aaatcgatgt gcaaggtgcc cgttcggtga
      241 aaaagaaatt cgaagacggc gtgtacattt tcatcctgcc gccctcgttc gatacgttac
      301 gcaccaggct ccaaaaccgg gccggagact cgccggaaga aattcaacgg cgcctgcaga
      361 aggccaaaga agaagtctgg agctaccgcg agtattacta catcgtccgc aatgacgact
      421 tgaaacaatc gctcaaggaa ctggaaagca tctttctggc ggaacgcatc aagaccaaac
      481 gcctgaacat gacctggctg gaagaaaagt ttattctcga caaagagggc aagcagggga
      541 attcctcctc cgcccccgca tcaaaggagc acatgcatca tgggtgaaat gttatcgttg
      601 ttaccggaat atgccaccgg cgaattcgac tcgcgccacc ggctggtgat cgtcgcggct
      661 caacgcgcca agcaattggt ccaaggggcc cgcacgaccg catcctccaa gttcacgaaa
      721 gaaaccagta tcgcactgga tgaggtgttg cggcacaacg tgaagttttt ggtcggcaaa
      781 gaagcccgcg atgcgatcaa ggaatccaag cgcgtgaagg aaggcgagac tgaacgccta
      841 gccatgatga ccggagaaga cgccaaggag atcaagaagg aactcagcgt ctacgttgac
      901 gataccgcca agcccgtcgc cgccccggag gccgaggagt agtgtccctt cgtgcagacc
      961 accggccctt cactttccgg tgtccgtctt gtgctcgccg tcacggggag cattgctgcc
     1021 tataaagcgg tcagtctgtt gcgcctgctc cggcgcgaag ccgcgacagt caacgtggtg
     1081 atgacggccg gcgcctgccg gttcgtcacc ccgctgacat ttgaagtatt gtccggcgcg
     1141 catgtggcga ccgacctctt cgaagcgcac caggagatgc ttcacctgtc tctcccggaa
     1201 caggcgcagg ccatcgtcat tgcgccggcc accgccaatt gccttgccaa agccgccctc
     1261 ggactcgccg acgacctcct gtccaccatg ctcctcacaa ctcaatgtcc ggtgattttc
     1321 gcgccggcta tggacgggga catgtggcag catcccaccg tcgtggagca tgtcgcgacc
     1381 ttgcgcgcac gcggagccat catcgtggaa cccgaggatg gtccgctggc atccggccga
     1441 caggggcagg gcaggttagc cgatgaggaa cgaatcctgg cagcggttca ccacgccgtt
     1501 catccgcgcc gagactggtc cggccgcaag gtgctcatct cagccggccc gacccaggag
     1561 gccatcgatc ctgtacgatt tctctccaac cggtcgtcag gaaaaatggg atacgccctg
     1621 gccgaagccg ccagggctcg tggcgcggac gtcgtgctcg tctcaggccc tacctcgatc
     1681 cccgtaccag ccggggtgga atactgtccg gtgatcacag ctgaagagat gcataaggcg
     1741 ctgacaaccc aattggattg gagcgatacg gtgatcatgg ctgccgctgt cgcagatttt
     1801 cgtcccgccc ggccatccgc acacaagatc aagaagcggc gcggtccggt cacgcatctg
     1861 gatctcgaag cgaccgacga tattctttgc gaattacgtg atcgccggac gagtcaggtg
     1921 ctggtcggat ttgccgcaga aactgatgat ctccttgccc acgccaaaga aaagctgcat
     1981 gctaaaggcc ttgatctgct ggtcgcgaat gacgtgaccg cgccagggtc ggggttcgga
     2041 tcggatacca accgggtctg gttgctggcc cgtgaagccg atcccgagga actcccgctg
     2101 ttatccaagc gcgacgtcgc ggaccggata ctggatcgaa tcctgacagt ccatacaggc
     2161 cggtaactcg agagcaaccg gtcgcagccg catcgcaagg agttacctgt gtcgtcacca
     2221 ctgcgcattc ctcccgccat cgccgcagag atcgatcgac tggccacagc cgtggcgaag
     2281 gatccacgat cgaaagactt cctgccgctg gcagatgaat acatcaaggc gggcatgtgg
     2341 caggaagccg cggccgtgct cgaagatggc ctgaaggtct atccaggatt cgtgaccgcc
     2401 atggcggcct tgggcagggt ctacgatcaa ctgggacaag cggaaaaagc caaggtcatt
     2461 ctcgaggagg tcgtgaaaca acgccccgac aatcttcggg cacaccgcat tctcgccaag
     2521 ctctatcatg cggagggaca ggccgacttg gcactccgtt cctgtacagc catcctcaac
     2581 gccaatccct tcgatgagga ggcgttggca gtcaaacgca gtgtcaccgg cgcgccggac
     2641 gagccgccgc catcgaaacg cgagaagaag cgcagcctcg cggaagcacc ggtggaaaag
     2701 atcacggcag cggtcccacc tgcgccggct ccggttgtcc ttgtatcaga gtccgcggcg
     2761 cctaccgtgt cgatgcccga tccgacgcct ccccagagcc ccccatctcc cgctgttcaa
     2821 catgcagccg caatcgcgcg cctcgaagcc tggctcagca ccatccaggc taaacgccac
     2881 cccgcagccc ggtgactgac acactcaccg ggactctttc agcgtttgac ccacccctga
     2941 cagcctgtta gaatgccgcc gctgctggca tccctatcca ttgcagccta ctgaca
//
LOCUS       DAOQEW010000334         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16827_length_2962_cov_8.435501, whole genome shotgun sequence.
ACCESSION   DAOQEW010000334 DAOQEW010000000
VERSION     DAOQEW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16827_length_2962_cov_8.435501"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLS35_19590"
     CDS             <1..998
                     /locus_tag="PLS35_19590"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNI20930.1"
                     /translation="LAMELRGRGVNARSFTGRQVGIHTDSAHTKARISRVTAERIKEA
                     LAAGVIPVVAGFQGINASSDVTTLGRGGSDLTAVALAAALKADRCIIYTDVDGVYTAD
                     PNIVPAARRLDKISYEEMLEMASLGAKVLQSRSVEFAAKYSVPVEVNSSFKEGKGTLV
                     TREDADMEGVMVSGVTGDRNQAKLTIVGVPDRPGIAARVFGAVANANIVVDMIIQNVS
                     QASMTDISFTVPKADLRNAVDLVQRLSQEIGARSVAVTESIAKVSLIGVGMRSHSGVA
                     AKMFEVLSREGVNIMMISTSEIKISCVIEEKYLELAMRTLHTAFGLDRVSAPALG"
     gene            1058..2716
                     /gene="cimA"
                     /locus_tag="PLS35_19595"
     CDS             1058..2716
                     /gene="cimA"
                     /locus_tag="PLS35_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNI20931.1"
                     /translation="MARTSMKRRTTASRRSSAPDSTTAPTLTAAQASALEIYDTTLRD
                     GAQAEDVSFSVEDKLRVAQQLDELGVHFIEGGWPGANPKDIEFFRQIKTMPFRNATVV
                     AFGSTRKASNPVRKDKNLQALLDSGTQTITLFGKSWSFQVTDALGIALAKNLELIEDS
                     IHYLRSKDRRVFYDAEHFFDGYKANPDYALQTIRRAIAGGAERVILCDTNGGTMPWEI
                     REICRIVKQECPVPLGIHAHNDSEMAVANSLVAVESGILQVQGTINGIGERCGNANLC
                     SIIPNLQLKMQRPALGNKLTRLKDVSGFVTEIANLMPNKRQPYVGDAAFAHKGGVHIH
                     AVLKNAATYEHIDPAVVGNRRRILVSDYAGRSGLMEKVEAYGISLTKNHAKLQELVET
                     LKERESQGYQFEGAEGSFELLMRKAMGSHRPSFQLLGFRVIVEKKQEHGLLLSEATVM
                     VKVGDVVEHTAAVGAGPVNALDHALRKALEKFYPQLREVKLLDYKVRVLSADKGTESK
                     VRVLIESGDHKDKWGTVGVSENIMEASWQALADSIEYKLLLAGR"
BASE COUNT          680 a          745 c          910 g          627 t
ORIGIN      
        1 tgttggccat ggagcttcgg gggcgaggcg tgaacgcccg atcgttcaca ggccgacagg
       61 tcggcattca taccgacagc gctcatacca aggcgagaat ttcccgtgtc acggccgaac
      121 gaatcaagga agcactcgcc gcaggggtca ttccggtggt cgcgggattt cagggcatta
      181 acgccagctc ggatgtcacg accttgggac ggggagggtc cgatttgact gctgtggcgt
      241 tagctgcggc cctcaaagcg gatcgctgca tcatttatac ggatgttgac ggggtgtaca
      301 ctgccgatcc caacatcgtc ccggctgcgc gtcgactcga caagatttcg tacgaagaga
      361 tgctggaaat ggcgagcctc ggcgcaaagg tgctgcagag ccgctcagtt gaattcgcag
      421 cgaaatattc cgttccggtg gaggtcaatt caagcttcaa ggaaggaaag ggaacgctcg
      481 tgacacgtga agatgccgat atggaagggg tcatggtgtc cggtgtgacg ggagaccgca
      541 atcaggccaa gcttacgatt gtcggtgtgc cggatcgtcc gggtattgct gcgcgtgtct
      601 ttggggccgt ggcgaatgcg aatatcgtgg tggatatgat tatccaaaac gtcagccagg
      661 cctccatgac cgatatctcc ttcacggtgc cgaaggcgga tttgcgcaac gccgtcgatc
      721 tcgttcagcg gttgtcgcag gagatcgggg cgcggtcggt tgccgtcact gaatctattg
      781 ccaaggtgtc tctgattggc gtgggcatgc gatcccattc cggagtggcg gcaaaaatgt
      841 ttgaggtgct gtcgcgcgaa ggcgtcaaca tcatgatgat cagcacatcg gaaattaaaa
      901 tctcctgtgt gatcgaagaa aagtacttag aattggccat gcgcacgctg catacggcgt
      961 ttggcctgga tcgcgtgtcg gcgccggcct tgggctaatt gtcgcgaggg gaacgtcgct
     1021 ggccgagcag ccttctcgac acgtcacgaa ccgccgtgtg gctcgcacat ctatgaaacg
     1081 ccgaacgaca gcatcgcgcc ggtcttccgc tccggactcg actaccgcac cgacgctcac
     1141 cgccgcacaa gcctctgcac tggagatcta cgatacgacg ctgcgtgatg gagcgcaggc
     1201 ggaagacgtc agtttctccg tggaagataa gcttcgcgtg gcgcaacagc ttgatgaact
     1261 cggtgtgcat ttcatcgagg gcggatggcc tggagccaat cccaaagaca ttgagttctt
     1321 ccggcagatc aaaaccatgc cgtttcggaa tgcgacggtc gtggcgttcg gctcgacacg
     1381 gaaagccagc aatccggtcc ggaaagacaa aaaccttcag gcgctgctcg attccggcac
     1441 gcagaccatt acgctcttcg ggaaaagttg gtcgttccaa gtgacggatg ctctgggcat
     1501 tgcgctcgcc aaaaatttag aactcatcga agattccatt cactatctgc gttcgaaaga
     1561 ccggcgcgtg ttctatgacg cggaacattt cttcgacggg tacaaggcca atcctgatta
     1621 tgcgctgcag accattcgtc gtgcgattgc cggcggtgcc gagcgagtga ttttgtgcga
     1681 cacgaacggc ggcacgatgc cgtgggagat tcgggaaatt tgccggatcg taaagcagga
     1741 gtgtccggtg ccactgggca ttcacgcgca caatgacagc gaaatggccg tggccaattc
     1801 actcgttgcg gtggagtctg gaattctcca ggtgcagggc acgattaacg gaatcggcga
     1861 gcggtgcggg aatgccaacc tctgctccat catcccgaat ctgcaattga aaatgcagcg
     1921 tccggccctc ggaaacaaac tgacgcgact caaagatgtc tcgggatttg tcacggagat
     1981 tgccaactta atgcccaaca agcggcagcc ctatgtcggg gatgccgcgt ttgcgcacaa
     2041 gggtggcgtg cacattcatg cagtcctcaa gaatgccgcg acctatgaac acatcgatcc
     2101 tgccgttgta gggaaccggc gccggatcct ggtttccgat tatgcgggac gtagcgggtt
     2161 gatggaaaag gttgaagcct acgggatttc gttgaccaaa aatcatgcga agctccagga
     2221 actcgtcgag acgctcaagg agcgcgaaag tcagggctat caattcgaag gcgccgaggg
     2281 ctcctttgag ttgttgatgc gcaaagcgat ggggagccat cgtccgtcat ttcagttgct
     2341 gggcttcagg gtcatcgtcg aaaagaagca ggaacatggg cttctgctgt cggaagcgac
     2401 ggtcatggtg aaggtcggag atgttgtcga acatacggcg gcggtggggg ccggacctgt
     2461 gaatgcactc gaccatgcat tacgcaaagc cttggagaaa ttttacccgc aactgcgtga
     2521 agtcaaactg ctggactata aggtccgcgt gttgtctgca gataagggga ccgaatccaa
     2581 agtacgggta ctgattgaat cgggggatca taaggacaag tgggggacgg tcggggtctc
     2641 tgaaaatatt atggaagcca gttggcaggc gttggcggat agtatcgagt acaaattact
     2701 gctagccggt cgctgaagcc gttttccctc cattcggcgc atataatcct tgacaggcca
     2761 gataacctca cgtaacttat ggggaaattc tttgtcttgg acgctcgaca ccaagaagca
     2821 cttcctaagg aacgactcta gtggatggcg gggggaaagg catgagaaag gcggatatcg
     2881 cgaacgaaat cttcaagcag gttggagtgt ctaaaaacga tgcagcggat atcgtcgaac
     2941 tcgtgctcaa tctgctgaag gg
//
LOCUS       DAOQEW010000335         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_16850_length_2960_cov_7.805508, whole genome shotgun sequence.
ACCESSION   DAOQEW010000335 DAOQEW010000000
VERSION     DAOQEW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16850_length_2960_cov_7.805508"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1494)
                     /locus_tag="PLS35_19600"
     CDS             complement(157..1494)
                     /locus_tag="PLS35_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNI20932.1"
                     /translation="MAQGIAQIRRSVLTLALPVTVSSLLQRAEGIVAVFLVGGLGAVP
                     IAAVGLGQLLAFIATTLVSGLSVGSNVVMAQLWGARRYDDAGHAARHFLGLSILIALG
                     LLSFGLLVNRLAMEMLGAQADVIRLALPYSNLIFLLIPFTILLQVLSSILQGTGDTKT
                     PMYAMIVVNLLHIAIAIPLIYGHWGLPALGINGAAIAVGIAEATGCLYLFTRCRPMLR
                     GSHRFRFDLIRTIWHVGAPVSGERVVQQAGILLYTKIVLMYGTVAYAAHQVGLSIESL
                     SFLPGYGFAIAAATMVGQSIGAGKYTRAKLETWEANRLAAYMMSAMGVVFFLFPYALL
                     RAFTSDEAVVELGTLFLKIVALLQIPLALTMVVAGSLRGAGDTRFVMIATMIGMWGVR
                     IPLAIVAGYWLSMGVLYVWLAMIADWTVRMALMLWRHRSERWRSIQVLRSSPT"
     gene            1597..>2960
                     /locus_tag="PLS35_19605"
     CDS             1597..>2960
                     /locus_tag="PLS35_19605"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNI20933.1"
                     /translation="MLILPEPAARSSYRLSKSRYLSGLQCHKRLYLEVHAPGLATKPD
                     AATQAILDMGTDLGELARQRFPGGRLVTAGYRQSQEALAQTAELLQDPTVPAIFEGAF
                     QFNQVLVRVDVLQRVGVNELGQPTWRLVEVKSSTKVKATHVDDLTIQSYVLEGLGLVL
                     AEICLMHVNTQYVFDGRYLDLDQLFGLRSLTETVRPRLPEVPARLAAMQAMLEGMSAP
                     AVMPDEHCQSPYECPFWTHCIEHKPARWIYHLPGSSKTVVQLRELGVETIDEIPDHVA
                     LTPVQRRVKDNREWIGEGLRSALETIVYPVHHLDFETFMPAVPKFVDTRPYQVIPTQW
                     SNHIEQPDGTLTHAEFLCRDERDPREELAITLLESLGQTGSICVYSSYERSVLERLAE
                     DFPSLRKDLKRVVARLWDLHVIIRDHYYHPAFEGSYSIKAVLPAVMPSLSYADLVIRD
                     GGVAA"
BASE COUNT          634 a          846 c          914 g          566 t
ORIGIN      
        1 aggtggcccc tatcgtttgg tgacgcctgg tctgggcgat ctctgcgcca atgtgaaggc
       61 cgtgggccgc atcgaagtac gagccggatc ggggaaggat acgcggccgg ccgtgcgtcc
      121 gccggaatgt gcagtggacg gccagagctg aatacctcaa gtgggagagg accggagcac
      181 ttgaatgctt ctccaccgct ccgaacggtg tcgccagagc atcaatgcca tgcgtaccgt
      241 ccagtcggcg atcatcgcca gccatacgta caacactccc atggaaagcc agtagcccgc
      301 cacgatggcc aggggaatcc gtacgcccca catgccaatc atcgtcgcga tcatgacgaa
      361 ccgggtatca cccgctcccc gcagagagcc ggccaccacc atggtgagtg cgagcggaat
      421 ctgcaggagg gccacgattt tcagaaacag ggtgcccaat tcgacgacgg cctcatcact
      481 ggtgaaggcg cggagcaacg cgtaggggaa caagaaaaac accactccca tggccgacat
      541 catgtaggcg gccagccggt ttgcttccca ggtttcgagc ttcgcccgag tgtatttgcc
      601 ggcgccgatg ctctgtccca ccatcgtggc tgctgcgatc gcaaagccat atcccggaag
      661 aaacgacagg gactcgatcg acaagccgac ctggtgggct gcgtaggcga cggtgccgta
      721 catcaacaca atcttggtgt atagcaaaat gccggcttgc tggacgacgc gctcacccga
      781 gaccggtgcc cccacgtgcc agatcgtgcg aatcaaatcg aatcggaatc ggtgggagcc
      841 gcggagcata ggccgacagc gtgtgaagag gtacaaacat ccggtcgctt ccgcgatgcc
      901 gaccgcaatg gctgcgccat tgatgcccaa ggccggcagg ccccagtgac catagatcag
      961 gggaatcgcg atggcaatgt ggagcagatt caccacgatc atggcgtaca tcggcgtttt
     1021 cgtgtcgccc gtgccctgca agatcgaaga gaggacctgc agcaggatcg tgaacggaat
     1081 gagcaggaag atcaggttcg agtaggggag cgcgaggcgg atcacgtccg cttgcgcccc
     1141 gagcatttcc atggccagcc gattgaccag aaggccgaag ctcaagagac cgagcgcaat
     1201 gaggatggag agtccgagga aatgacgcgc ggcatggcct gcatcgtcgt aacggcgggc
     1261 gccccacaat tgcgccatga ccacattgga tcccaccgaa agaccggaaa ccagggtcgt
     1321 agcgataaag gccagcaatt gaccgaggcc gacggcggcg atcgggacgg cccccaatcc
     1381 tccaacgaga aagacggcga cgatgccttc agcccgttgc aacaggctac tgacggtgac
     1441 gggcagtgcg agcgtgagaa ccgagcgtct gatctgtgcg atgccctgtg ccatgcggca
     1501 tcatcctaac agaaccgggc tcgcttcgtg gagtttctcg ccggctgggc gggactggac
     1561 aactcacgtc atgcttcggt agcctgcaca cagactatgc tgatcttacc tgagcctgcc
     1621 gcgcgctcct cctatcgttt atccaaatcg cgttatctat cgggtctgca atgtcataag
     1681 cgtctgtacc tcgaggtgca tgctccgggg ttggcgacca aaccggatgc cgccacgcag
     1741 gcgattctgg atatgggcac ggatctgggt gaactggcgc gtcaacgttt ccccggcggc
     1801 cgtctagtca cggccgggta tcgtcaatct caggaagccc tggcgcaaac ggctgagctg
     1861 ctacaggatc cgacggttcc ggcgatcttc gaaggggcct ttcagttcaa ccaggttctg
     1921 gttcgtgtgg atgtcctgca acgtgtcggg gtgaatgagc tgggacaacc gacctggcgg
     1981 ctcgtggagg tgaagtcttc caccaaggtg aaggcgacgc acgtggatga tctgacgatc
     2041 caaagctatg tcctggaagg acttggactg gtgctggcgg aaatctgcct gatgcatgtg
     2101 aatacgcagt acgtctttga cgggcggtat ctcgatctcg accagctctt cggccttcgt
     2161 tcactgacgg agaccgtgcg tccgcgatta ccggaagtgc cggcgcgact cgctgccatg
     2221 caggccatgt tggaaggcat gtcggctccg gccgtgatgc cagatgaaca ttgtcaaagc
     2281 ccctatgagt gtcccttctg gacccattgc atcgaacaca aaccggctcg ttggatctat
     2341 catttaccgg gaagtagcaa gacggtcgtg cagcttcgtg aattgggcgt tgagacgatc
     2401 gacgagattc ctgatcatgt cgcactcacg ccggtgcaac ggcgggtgaa agataaccgg
     2461 gaatggatcg gcgaagggct gcggtcggcg ttggagacga tcgtctatcc cgtccaccat
     2521 ctggattttg agaccttcat gccggcggtg ccgaaattcg tggataccag accctaccag
     2581 gttattccca cacagtggtc caatcacatt gagcagcccg acggcacgct cacgcatgcc
     2641 gagtttctgt gccgggatga acgggatccc agggaagagt tggccatcac gttattggaa
     2701 tcactcggcc agacgggcag catctgcgtc tattcgagct atgaacgatc ggtacttgag
     2761 cggctggcgg aggatttccc gtcgttacgg aaggatctca agcgggtagt ggcgaggctg
     2821 tgggatctcc acgtgatcat ccgggatcac tattatcatc ctgcctttga aggcagctac
     2881 tccatcaaag ccgtattacc cgccgtcatg ccgtccctga gttatgccga tctggtgatt
     2941 cgggatgggg gtgtcgcggc
//
LOCUS       DAOQEW010000336         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17138_length_2934_cov_7.295589, whole genome shotgun sequence.
ACCESSION   DAOQEW010000336 DAOQEW010000000
VERSION     DAOQEW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17138_length_2934_cov_7.295589"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1243
                     /locus_tag="PLS35_19610"
     CDS             5..1243
                     /locus_tag="PLS35_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20934.1"
                     /translation="MLRRILILVFVISLPAIMPTLAPAQAMPQQLRVIDTPSDGGGSL
                     TVLWAPSATDSATTKYQILVHEGGLPQDPTAWKVVAEFPANTRYVREGKSAWWTRSGD
                     PNDHLFLIKNGKGLEVKSGQPYAVTVASVNGKERLIAPPVIASAEPNWMNWNQVNNLV
                     LALMFGAIVFYAINLAKRKDIFLRRIPGLDAVDEAIGRATELGKPVLYMTGAHDMNDP
                     STIAAAVILGRVAKKAASYETELLVPHREPITMAVCQEITKQAYMEAGKPDLFKDDAN
                     FFITSDQFSYTAAVDGIMLRRKPAANFFMGSYFAESLLLTETGASTGAIQIAGTDSDH
                     QLPFFVTTCDYTLIGEELYAASAYLSKEPIQIGTLRGQDLGKAFILSVIGIGTLLATV
                     AVLTGNGWAQPLLDLFKDLK"
     gene            1240..1890
                     /locus_tag="PLS35_19615"
     CDS             1240..1890
                     /locus_tag="PLS35_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20935.1"
                     /translation="MIFLRRQLPLLITMITGLVMAAQYYVPHPASEQLLTTVTKWLQI
                     IGGFALVLGITSLFHVHAAKIRRKEAGWAYSLVLYVGMLGTIIVGLWNNGKESIDGAL
                     TSFGWVYSYTLVPLQGTMFAILAFFIASAAYRSFRARSREAAVLLVAAVIVMMGRVPL
                     GEYILPLSGDLSSWILNVLNASVRRAILIGVSLGAVALSFKIIFGVERSYLGGGKE"
     gene            1887..2732
                     /locus_tag="PLS35_19620"
     CDS             1887..2732
                     /locus_tag="PLS35_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20936.1"
                     /translation="MTFAERMLRIDRRIIFLVIGLCTLVPLLFPVGLPIKISSEVRGV
                     YDYIEGLPEGSVFLLSLDFDPASKPELYPQAIAILRHAFKKNLRVVAMTLWVSGTGLA
                     DQILTQTAKEAGKENGKDYVFLGWSPGGSAVILNMGQDLYNAFPADYGGKATKGLPVL
                     AGVQNLRDVTYAVSLGAGNPGVEAWYVFGKDKYKFELGGGCTGVIAPGLYPLLRSGQI
                     NGLIGGLRGAAEYETLIGQKGKAVAGMDAQSATHLAIIVLVAICNIFYFSLRRQQRRQ
                     DGMGS"
     gene            2738..>2934
                     /locus_tag="PLS35_19625"
     CDS             2738..>2934
                     /locus_tag="PLS35_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20937.1"
                     /translation="MELSFGVILGAWIATGLTLFILSFLYEDNPLFKLAEHLYVGVSL
                     GYTIVKTYDTVIMTLIVRPIL"
BASE COUNT          565 a          910 c          858 g          601 t
ORIGIN      
        1 cctcttgctg cgccgcatcc tcatcctggt ctttgttatc agcttgccgg caatcatgcc
       61 gacgctcgcc ccggcgcagg ccatgccgca gcaacttcgc gtgatcgata cccccagcga
      121 cggaggcgga agcctcacgg tgctctgggc tccctcggca accgacagtg ccaccacgaa
      181 atatcaaatc ctcgtccatg agggcggcct tccccaggac ccaacggcgt ggaaggtcgt
      241 ggcagaattt cccgccaata cgcgctacgt ccgcgagggc aagtcggcct ggtggacgag
      301 atcaggtgat ccgaacgatc acctctttct aatcaagaat ggcaagggtc tcgaggtgaa
      361 atccggccag ccctatgccg tcaccgtcgc gtcggtcaac gggaaggaac gattgattgc
      421 gccgcccgta atcgcctctg cagaacccaa ctggatgaac tggaaccagg tcaacaatct
      481 tgtgttggcg ttgatgttcg gcgcgatcgt gttttacgcc atcaatctgg ccaagcggaa
      541 ggacatcttt ctccgccgca ttcccggtct ggatgccgtc gatgaagcga tcgggcgggc
      601 cacggaattg ggcaagccgg tgctgtacat gaccggcgcg catgacatga acgatccctc
      661 cacgattgcc gccgccgtga tcctgggacg tgtcgccaag aaagccgcct cctacgaaac
      721 ggaattattg gtgccgcatc gagagccgat cacgatggcg gtctgccagg aaattaccaa
      781 gcaggcatac atggaagcgg ggaaaccgga tctgttcaag gacgacgcga actttttcat
      841 caccagcgac cagttcagct acaccgcggc ggtcgacggc atcatgctcc ggcggaaacc
      901 ggcggcaaac tttttcatgg gctcgtactt tgccgaatcg ctgctgctga cggaaaccgg
      961 cgccagcaca ggagccattc aaatcgccgg caccgattcg gaccaccagt tgccgttctt
     1021 cgtgacgacc tgcgactaca ccctgatcgg cgaagaactc tacgccgcca gcgcgtacct
     1081 ctcgaaggag ccgatccaga tcggcacgct gcgcggacag gatctcggca aggccttcat
     1141 tctgagcgtc atcgggatcg gaacgctgct ggccaccgtc gccgtactca ccgggaacgg
     1201 gtgggcgcag ccgctgctgg acctgtttaa ggatctcaaa tgattttcct tcgccgacaa
     1261 ctgccgctgc ttatcaccat gatcacggga ctggtcatgg cggcccaata ctacgtgcca
     1321 catccggcct ccgaacaact gcttaccacc gtgacgaagt ggctccagat catcggcggc
     1381 ttcgcgctcg tcctcggcat caccagcctg tttcatgttc atgcggccaa gatccggcgg
     1441 aaggaagccg ggtgggccta tagcctggtg ctttatgtcg gcatgctcgg cacgatcatc
     1501 gtgggactct ggaataacgg caaggaaagt atcgacggcg cgttaacgtc gttcggctgg
     1561 gtctactcct atacgctcgt gccgctgcag ggcaccatgt tcgccatcct ggccttcttc
     1621 atcgcctctg ccgcgtaccg ctcgttccgc gcgcggagcc gtgaagccgc cgtgctgctg
     1681 gtggccgccg tcatcgtgat gatggggcgg gtgccgctcg gcgaatacat cctgccgctc
     1741 agcggcgacc tgtcgagctg gattctgaat gtcttgaacg cctccgtgcg gcgggcgatt
     1801 ctgatcggcg tcagccttgg cgcagtcgcc ctttcgttca agattatctt cggcgtcgaa
     1861 cgttcgtacc tcggcggggg caaggaatga cgttcgccga gcgtatgttg cgcatcgacc
     1921 ggcggatcat ctttctggtg atcggcctct gcaccctcgt gccgctgctg ttcccggtcg
     1981 gattaccgat caagatttcg tccgaagtgc gcggcgtcta cgactatatt gaagggctgc
     2041 ccgaaggatc ggtctttctc ttgtcgttgg acttcgaccc cgcatccaaa ccggaactct
     2101 acccgcaagc catcgcgatt ctgcggcacg ccttcaagaa aaatctccgc gtcgtagcga
     2161 tgacactctg ggtctcaggc accggactcg ccgaccagat cctgacgcaa acagccaagg
     2221 aggcgggcaa ggagaacggg aaggactacg tcttcctcgg ctggagtccc ggcggcagcg
     2281 ccgtcattct caacatggga caggatctct acaacgcctt tccagcggac tacggcggca
     2341 aggcgaccaa aggcctgccg gtgttggctg gcgtgcagaa cctgcgcgac gtcacctatg
     2401 ccgtgagtct cggtgccggg aatcccggcg tcgaagcctg gtacgtcttc ggcaaagaca
     2461 aatataagtt cgaactcggt ggcggctgca ccggcgtcat cgcgccgggc ctctatccgc
     2521 tgttacgcag cgggcagatc aacgggttga tcggcggtct gcgcggcgcg gcggaatatg
     2581 agaccttgat cgggcagaag gggaaagcgg tcgccggcat ggacgcgcaa tcggcgacgc
     2641 atttggccat catcgtgctc gtcgccatct gcaacatctt ttatttctcc ttgcggcggc
     2701 aacagcgccg gcaggacggg atgggatcct aggcactatg gaactgtcgt tcggtgtcat
     2761 actcggtgcc tggatcgcca caggcttgac cctcttcatc ctctcgtttc tctatgagga
     2821 caatccgctg ttcaagctcg ccgaacatct ctatgtcgga gtctcgctcg gctatacgat
     2881 cgtgaagacc tatgacacgg tgatcatgac cttgatcgtg cgccccattc tcga
//
LOCUS       DAOQEW010000337         2925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17226_length_2925_cov_6.343554, whole genome shotgun sequence.
ACCESSION   DAOQEW010000337 DAOQEW010000000
VERSION     DAOQEW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17226_length_2925_cov_6.343554"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLS35_19630"
     CDS             <1..412
                     /locus_tag="PLS35_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNI20938.1"
                     /translation="SVYLLVGLVLLGKHVRVLDILALGEEPARSLGINTERTKRVIFL
                     LSALMTGAVVSFSGMIGFIGMIIPHAVRLVVGADHRLLLPASTLVGGMFLMVADTMAR
                     TLFVPSEVPVGVITALAGGPFFVYLLVWRKDRLA"
     gene            409..1305
                     /locus_tag="PLS35_19635"
     CDS             409..1305
                     /locus_tag="PLS35_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNI20939.1"
                     /translation="MSRHLQGKARIDTGAGIGTDAPLAYDVCGAYFCYGRLSTLDVRW
                     VLQNVTVQVQSGEILGIVGPNGSGKTSLLKLLAKLAAPQRGDITLFGQSLAGLSQDDT
                     ARAVACVPQENPQLFPFTVAETVLMGRFPHRHRSRWSLGFGWEDQDDCRAAAQAMETM
                     DITHLASRLVTDLSGGERQRTMIARALAQMPRVLLLDEPTAFLDLQHQLDICSVVRRL
                     REERGLTVVIVSHDLNLASQYCDRIAMLKDGRVAAIGTPSEVMSVETLREVYGCTVLI
                     DAHPDSGLPRITLPGPAVPVHR"
     regulatory      1371..1627
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1656..>2925
                     /locus_tag="PLS35_19640"
     CDS             1656..>2925
                     /locus_tag="PLS35_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNI20940.1"
                     /translation="MLKRVREWSVCCLLLLIVSTPPVWPAVAGAEEPEEPEPVVQAEE
                     VVISATKTPVPVTQVTSAVEVITAQDMKKQNIRHVADALRLAQGLAVFSSGGPGTEVN
                     ARIRGGGSSQTLVLIDGAIVNSGTLGSYDFSNLTTDNIERVEILRGAQSMLWGSDAMG
                     GVINIVTKRGEGPLTATGFMEYGSFATLREGGTVSGKQGLVDYSLSLSRWDTSGFSAV
                     NYRRGATERDSFRNWQGSGRIGVDLPRDGRLDFTMRWMNSDTQLDNVSATFPADVYGS
                     KMRSQQYVFSGSYEQPITGWWSQKLTLARSQEASLFLPGTVQRSLVDGFISTPFGSPN
                     ETRVLSNRLEWQHNFQVTRLLLLSAGYQFREQQGENDTGLINRVLSSHAGFAQAQFNL
                     WDRVFATAGVRHDSYNVFGNATTYRLTGGDY"
BASE COUNT          563 a          815 c          938 g          609 t
ORIGIN      
        1 ctccgtctat ttgctcgtcg gcctcgtcct gctggggaag cacgtccggg tgctcgacat
       61 tctcgccttg ggtgaagaac ccgcgcgatc gttgggtatt aacacggaac ggaccaagcg
      121 cgtcatattc ctcttgtcag cgctcatgac cggggctgtg gtgtccttca gcggcatgat
      181 cgggttcatc ggcatgatca ttccacatgc ggtgcgtctg gtggtggggg cggatcaccg
      241 attgctcctg ccggcatcca cgttggtcgg agggatgttc ctgatggtgg cggataccat
      301 ggcacgtacc ctgttcgtcc catccgaagt tccggtcggc gtcattacgg cgttagcggg
      361 tggtcccttc ttcgtgtact tgctggtctg gagaaaggat cgtctggcgt gagcaggcat
      421 ctccagggaa aagcacgtat cgataccggg gccggcattg gtacggatgc ccccttggcc
      481 tatgatgtgt gcggagccta tttctgctac ggcagactca gcacgctcga cgtcaggtgg
      541 gtgctccaga atgtgacggt gcaggttcaa tctggcgaaa tcctcggcat cgtcggtccc
      601 aatggttcgg gcaaaacctc tctgttgaaa ctattggcca agctcgcagc acctcaacga
      661 ggggacatca cattgttcgg tcagagcctg gctggcctgt cgcaggacga cacggcgcga
      721 gccgtcgcgt gtgtgccaca ggagaacccg cagctatttc catttacggt ggcggaaacc
      781 gtgttgatgg ggcgattccc gcatcgccat cggtcgcgct ggagccttgg tttcgggtgg
      841 gaggaccagg atgattgtcg ggcagccgcg caggccatgg agacgatgga tattacgcat
      901 ctcgcctcac ggctggtcac cgatctctcc ggcggtgagc gccagcgaac gatgatcgca
      961 cgggccctcg cccaaatgcc tcgcgtattg ctactggatg agccgacggc gtttctcgat
     1021 cttcagcatc agctcgacat ttgctcggtt gtgcgtcgat tgcgtgagga gcgggggttg
     1081 acggtggtca tcgtgtcgca tgatttaaat ctggccagtc agtactgcga tcgcatcgcg
     1141 atgctgaaag acggcagagt ggccgcgatc gggacgccct cagaggtcat gtcagtcgaa
     1201 accttgcgtg aggtctacgg ttgcaccgtg ctgatcgatg cgcatcccga ttcgggattg
     1261 ccgcgtatca cactgcccgg gcctgccgtg ccggttcacc gataggaacg gaacgttgcg
     1321 gaggggttcg tgcccggccc acccgcgtga tgagaagggg catgtcatca ttggagaacg
     1381 gagtatcagg aagacgccgt cagcgtcggg gaagatggtg caaaaccatc acggtgccgc
     1441 cactgtaagc ggggagtgac cctgcaggaa gccactggcg agcgacgtga ttcgtgaatc
     1501 atcccttgtg aagcgcaaga ggtttcctct gaccgtttca cgatgactca cgtctcacgc
     1561 cgtcgggaag gcgcagggaa gccaggatcc gcaagtcagg agacccaact gacgggtgtt
     1621 acgactgagc ccttcgagcg aaagggaacg tcagcatgtt gaagcgtgtc agggaatggt
     1681 ctgtctgttg tctgttgctg ttgatcgtga gcaccccgcc tgtctggccg gcggtcgccg
     1741 gtgccgaaga accggaggag cctgaaccgg tcgtgcaggc cgaagaagtc gtcatctcag
     1801 ccacgaaaac accggtccct gtgacgcagg tgaccagcgc cgtcgaagtc atcacggcgc
     1861 aagacatgaa aaagcaaaat atccggcacg tggccgacgc cctacggttg gcgcaagggc
     1921 tggccgtgtt ttcgagcggc ggaccgggaa cggaggtcaa cgcaagaatt cgcgggggag
     1981 gttccagcca gacgctggtc ctgatcgacg gcgccatcgt caacagcgga acgttgggca
     2041 gctatgattt ttccaacctg accaccgaca acatcgagcg agtcgaaatc ctgcgcggcg
     2101 cgcaaagcat gttgtggggg tcggatgcga tgggcggcgt gatcaatatc gtcacgaagc
     2161 gaggcgaggg gccgcttacc gcgaccggtt tcatggaata tgggtccttc gcgacgttgc
     2221 gcgagggcgg caccgtatca ggaaagcagg gactcgtcga ctacagcctt tcgctgtccc
     2281 gttgggacac ctccggcttt tcggccgtca attatcgccg cggcgcgacc gagcgggatt
     2341 cctttcgtaa ttggcaggga tcggggcgca tcggcgtgga tctcccgcgt gatggtcgac
     2401 tcgatttcac catgcgctgg atgaattccg atacccaact cgacaatgtc tcggcgacat
     2461 ttccggcgga tgtctacggg tcgaagatgc gcagtcagca gtatgtgttc agcgggagtt
     2521 atgagcagcc gatcaccggc tggtggtccc agaagctgac actcgcacga tcgcaggaag
     2581 cttcgctgtt tctgccgggg actgtacagc ggagccttgt cgacggattc atcagcacgc
     2641 cgttcggatc tccgaatgaa acgcgtgtcc tttccaatcg cctggagtgg caacataatt
     2701 tccaggttac caggctgctc ctgctcagcg cagggtatca attccgggaa cagcagggtg
     2761 aaaacgacac gggcctgatc aatcgcgttt tgagttccca cgccggattt gcgcaggcgc
     2821 agttcaatct ctgggatcgt gtcttcgcga cggccggtgt gcggcacgac agttacaacg
     2881 tcttcggcaa tgccaccacg tatcgactca ccggcgggga ctacc
//
LOCUS       DAOQEW010000338         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17487_length_2900_cov_8.908260, whole genome shotgun sequence.
ACCESSION   DAOQEW010000338 DAOQEW010000000
VERSION     DAOQEW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17487_length_2900_cov_8.908260"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1304)
                     /locus_tag="PLS35_19645"
     CDS             complement(243..1304)
                     /locus_tag="PLS35_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20941.1"
                     /translation="MRERRWETAGRLTFQEILAVGERLGALGLKPAHPAKDVICYVED
                     WAVDSPEAFDQLDHWATEDVTLLHIREAWQGDFFLLAGGYHTVYQRFQDVGTYCSISH
                     PWRTKPGLRFHHPTSMFWLGFRHNHAFIRVRLHTQEVVTPGETREDARRAEWVDERRA
                     IFLDAIAMLDLPVETSVDKGSLVLTPADPATPFFCSWPDAFGPCQFEYNSLDAYEFLV
                     PASRLAATMASQPAGVRAYLTGFSDQALDAFAAVQPGAKTVYRCSVHCALGELPEVLE
                     TIAPQGRLYSTLCEFQTQELMPEGEDASAIIGIVGTAGGFQLEARLNRAPLPEEHMAG
                     WLERLLGMTVVYAPLPPFM"
     gene            complement(1350..2000)
                     /locus_tag="PLS35_19650"
     CDS             complement(1350..2000)
                     /locus_tag="PLS35_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20942.1"
                     /translation="MIAQGCRIAGQVCIAVGLALLWGCAGTGEMVSLNVQPQQPFVQS
                     GQPEPLKIVIEPFEDRRADKTKIGQRTHLGGGVTNFNVSGGSPGVTIAEALAEALRWR
                     GWNRRGWDARVVQSGVGVSGADIVIGGEIVDFSANAKSRVFNTKLTGESRLVIRAKNM
                     SDESSTQRNLQGEQSKLVFWFTSEDVENLMSGLLQDGIERFIADTKIEGRALKSAK"
     gene            complement(2134..2763)
                     /locus_tag="PLS35_19655"
     CDS             complement(2134..2763)
                     /locus_tag="PLS35_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20943.1"
                     /translation="MASPGADSLLTSDELRLVGDRDLFRSKERTILKVQALLTTLQAG
                     LQADLAHMTLLFPPEFDPAKAQSVKGERLEQCPYQYLDYPKHFHGDDKFTFRSLCWWG
                     HHLVFAMIVEGGQAKQCKKNFFDRFHQLAGQGLELSLAPTLWEWKRGEGYTLPITHDR
                     KAQLAAVLSGRAWFKIARFVPLDSPALREGRLPEIGRETFRSLFPLLSP"
BASE COUNT          639 a          897 c          822 g          542 t
ORIGIN      
        1 aggcgattgt caaacgaaga gaggaatcag gcgatgaaat tggaaggcac gatcgagtca
       61 caataggcat gtcgcgggtc acgctacagc aaaaggatct agcattcacg gtttagccgg
      121 ccgctcaaca cgggcatcca gcaaggccgc aggggagaaa gaaccggagg cgtacccgct
      181 gcggtacgtc gaggattctt tcgacctgag aacgcagctg gggtccgtgt tcagcggccg
      241 gactacatga agggagggag gggcgcatag actaccgtca tgcccaatag tcgctccagc
      301 caaccggcca tatgctcctc gggtagtgga gcgcgattga gccgggcctc cagttgaaac
      361 cctccggctg tgccgactat cccgatgatg gccgacgcat cttctccctc gggcatcaat
      421 tcctgcgttt ggaactcaca gagggtactg tagagacggc cctgtggcgc gatggtctcc
      481 aacacttcgg gcaattcacc caacgcgcaa tgtaccgaac agcggtaaac cgtcttcgcg
      541 cccggttgca ccgcggcaaa ggcatccaat gcctggtcgg aaaatcctgt gagataggct
      601 cgcacaccgg ccggctgcga cgccatggtc gcagccaatc gactcgccgg cacaagaaat
      661 tcataggcgt cgagtgagtt gtattcgaac tgacaggggc cgaaggcatc cggccaggaa
      721 caaaaaaacg gcgtcgccgg gtctgcgggc gtcagcacca ggctgccctt gtcgacggat
      781 gtctccaccg gcagatccag catggcgatg gcatcaagaa agatggcccg ccgctcatcc
      841 acccattcgg cccgccgtgc atcctcccgg gtttctcccg gcgtcaccac ttcctgcgta
      901 tgcagacgca cccggataaa ggcatggttg tgacgaaacc ccagccagaa catggaggtg
      961 ggatgatgaa accgcaaccc gggtttcgtg cgccagggat gactgatgga gcagtaggtg
     1021 ccgacatcct gaaagcgctg atagacggtg tggtaaccgc ccgccagcag aaaaaaatca
     1081 ccctgccagg cttcgcggat gtggaggagg gtgacatctt ccgtggccca atgatcgagc
     1141 tgatcgaagg cctcgggaga gtcgacggcc cagtcctcca cgtagcagat cacgtctttg
     1201 gcgggatgag ccggcttgag gcccaaagcc cctagacgct cgccgaccgc caggatctcc
     1261 tgaaaggtca gccggccggc ggtctcccag cggcgttcac gcacaacgct cctccgcggt
     1321 ccgcgcgccg ctgtcgaatc tggcgggcct cacttggccg acttcagcgc gcggccctca
     1381 atcttcgtat cggcgataaa ccgctcaatg ccgtcctgca aaagaccaga catgaggttc
     1441 tcgacatctt cgctggtaaa ccagaacacc agcttgcttt gttcaccctg gagattgcgc
     1501 tgcgtgctgc tctcatcgga catattcttg gctcgaatca ccaggcgact ctcgcccgtc
     1561 aatttcgtat tgaacacgcg gctcttggca ttggccgaaa aatcgacaat ttctccaccg
     1621 atgacgatat ctgcaccgga gacgcccacg cccgactgca ccacgcgcgc atcccatcca
     1681 cgacggttcc agccccgcca ccgcaaggcc tcggccaagg cttcggcgat cgtcacccct
     1741 ggcgagcctc cgctgacatt gaaattcgtc accccgccac ccaaatgggt gcgctggccg
     1801 atcttcgtct tgtccgcgcg acgatcctca aacggttcga tgacgatttt cagcggctcc
     1861 ggctggccgc tctgcacaaa cggttgctgc ggttgcacat taagcgacac catttctccg
     1921 gttccggcac agccccagag cagcgccaac cccaccgcaa tacagacctg acccgcgatt
     1981 cgacatccct gtgcaatcac acatgcctcc tgtcagtatg tggccttact aaaacgcgcc
     2041 cagtctaccc aactcgcagc cggatgacaa acgggaaagg gctggtttca gcaggaaaat
     2101 tcgccggtgg tccacgtcaa aacatgaagc tggttagggg ctgaggagag ggaagaggct
     2161 tcgaaaggtc tcccgcccga tctccgggag gcgtccttcg cgtaaggccg gcgaatccaa
     2221 cggcacaaac cgggcgatct tgaaccaggc ccgcccggac aatacggcgg ccaattgagc
     2281 cttccggtcg tgggtgatcg gcaacgtgta gccctcgccc cgcttccatt cccataacgt
     2341 gggcgccagc gaaagctcca agccctggcc cgccaactgg tgaaaccggt caaagaagtt
     2401 ctttttgcac tgcttcgcct gaccaccctc gacgatcatc gcaaacacca gatggtgccc
     2461 ccaccagcat aacgagcgga aggtaaactt gtcatcgccg tgaaaatgtt tcggatagtc
     2521 cagatattga taggggcact gttcgagacg ttcccctttc acggattgcg ccttggctgg
     2581 gtcaaactcg ggaggaaaca acaatgtcat gtgagccagg tccgcctgta accccgcctg
     2641 aagcgttgtc agaagtgctt gtaccttcag gatcgtccgc tctttggaac ggaacaggtc
     2701 gcgatcgccg accagtctga gctcatccga cgtcaacaac gaatcagcgc cggggctagc
     2761 catgggatcc ctctctgtca ttgtttactg agatcagtgg ccggagtgag acaggtgtgt
     2821 ggtcacgggt gatgcgtgac ggtcacgatt gggatttctg aagagattca aacgctgaga
     2881 cctgcatgtc aaatatgcgc
//
LOCUS       DAOQEW010000339         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17585_length_2891_cov_8.229549, whole genome shotgun sequence.
ACCESSION   DAOQEW010000339 DAOQEW010000000
VERSION     DAOQEW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17585_length_2891_cov_8.229549"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLS35_19660"
     CDS             <1..577
                     /locus_tag="PLS35_19660"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNI20944.1"
                     /translation="NLAMNYEYSTGRLAVLALVAQLEQQAIDAVVFLGDGEDLVSIAH
                     ELGRRGMSPIVLSATGMVGPRARAVPSSLLPRIFLVTPIDPPTEREVTYLRALSEQPS
                     LSSIGFARIAHGAVSLLAESLKQAGRRLDRQAMVDALEACREYDTGAGFSVTYGVHRR
                     LGRVASKVVGLMSDPPYISPVTGWMVPEGEP"
     gene            755..1114
                     /gene="rpsF"
                     /locus_tag="PLS35_19665"
     CDS             755..1114
                     /gene="rpsF"
                     /locus_tag="PLS35_19665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNI20945.1"
                     /translation="MELYESLFIIRPTLSDEETTTLIEKMKGIVTKNGATLVRAENWG
                     RKKLAYEIKRERKGTYVYFYFQGPGTTIADLERAYRLEDSIIKFLTVKLEQEPAPPRG
                     APVAAPAAQGATVGGVQ"
     gene            1098..1499
                     /locus_tag="PLS35_19670"
     CDS             1098..1499
                     /locus_tag="PLS35_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNI20946.1"
                     /translation="MAGFNKVILVGNLTRNPELRYTPSGTPVASFGLATSRRFKQGDE
                     LKEEVCFIDIVVFGKQAEHCGQYLSKGNGVIIDGRLQQRRWETEDGQKRSKHEVVAQG
                     VTFLPKRGEAGAGADSGGLHDEPPSYEDEQF"
     gene            1515..1772
                     /gene="rpsR"
                     /locus_tag="PLS35_19675"
     CDS             1515..1772
                     /gene="rpsR"
                     /locus_tag="PLS35_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNI20947.1"
                     /translation="MERGDRGNSGGGGGRFFQRRKPCRFCVDKAPIDFKDVGLLRNFL
                     TERGRIVPRRISGNCLQHQRVLTVAVKRARQIALVSFAEER"
     gene            1790..2461
                     /locus_tag="PLS35_19680"
     CDS             1790..2461
                     /locus_tag="PLS35_19680"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNI20948.1"
                     /translation="MSVESRGPAACLLIDTGEDRLVMTVPMDMLNPAIVDIGPEGLPL
                     SCSASGPQLGLDEPEDKFDGPLAIQLELSQQEGRISVTGTLEGTAIRQCVRCLSEYAD
                     PLLVSLYAEYLPQASVAAKSAPAEQGRRNTRRGAQPVEPEEEADEEDELYWYQGDHLD
                     LVPMLREQVILAAPMQPICREDCPGLCPQCGQNLNERRCDCPPAQVPSPFRVLRGRGP
                     KGENA"
     gene            2500..2685
                     /gene="rpmF"
                     /locus_tag="PLS35_19685"
     CDS             2500..2685
                     /gene="rpmF"
                     /locus_tag="PLS35_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNI20949.1"
                     /translation="MPNPKHKHSRSRRDMRRTAKTRLVPPGFSLCPQCHELKLPHYTC
                     MNCGTYKGKAVIVVEES"
BASE COUNT          602 a          773 c          915 g          601 t
ORIGIN      
        1 gaatctcgcg atgaattacg agtactcgac cggtcgtctg gcagtgctgg ccctcgtggc
       61 acaattggaa cagcaggcaa tcgatgccgt ggtatttctg ggagatggag aggatcttgt
      121 ctcgatcgca catgagctgg gtcgccgggg aatgtcgccg attgtattga gtgcaaccgg
      181 catggtgggg ccacgggctc gggctgttcc gtcgtctctt cttccccgga tattcctcgt
      241 gaccccgatt gaccctccta cggagcgaga ggtgacctat ttgcgggcgt tgagcgagca
      301 gccgtctcta tcgagcatcg gctttgcacg catcgctcac ggcgccgttt cgttgctggc
      361 cgaatcactc aagcaggccg gcaggcgtct cgaccgtcag gcgatggttg atgcgctgga
      421 ggcatgtcgc gaatacgaca ccggggcagg gttttcggtg acgtacggcg tgcaccgccg
      481 tctgggacgc gtggcttcaa aagtggtcgg tctgatgtcc gatccaccat atatctcgcc
      541 ggtgacgggc tggatggtgc cggaaggaga gccctgacgt cgataccgca cggtttcgtc
      601 gccggaaaga tcggccagga ggtgccgcgg cagagcgata aggttgacag gtctaaaaat
      661 cctatggtac tttccacggc tcatgtcggc tgatccggca tgcctacacc tcgctcccgt
      721 ctatgatggg ggccaatgtc caggaggaat ctgcatggag ctctacgagt ccctgttcat
      781 tattcgtccg actttaagcg acgaagaaac gacgacgctg attgaaaaaa tgaagggtat
      841 cgtcaccaag aacggcgcca cccttgtgcg agccgagaac tggggccgca agaagctcgc
      901 gtatgaaatc aagcgtgagc gtaagggcac gtacgtctat ttttattttc agggtcccgg
      961 taccacgatc gccgacctgg agcgcgccta tcgccttgag gattcgatca tcaagttcct
     1021 cacggtgaaa ttggagcagg agcctgctcc gcccaggggc gcacctgtgg cagcaccagc
     1081 cgcacaagga gcgaccgttg gcggggttca ataaagtcat tctggtcgga aatctgactc
     1141 ggaatccgga actgcggtac acacccagcg gaacgccggt cgcgagcttc ggcttggcga
     1201 cgagccggcg cttcaagcag ggtgatgaac tcaaggaaga agtctgcttc atcgatatcg
     1261 tcgtgttcgg caagcaagcg gaacactgcg ggcagtacct cagcaaaggc aacggggtga
     1321 ttatcgatgg gcggcttcaa cagcgccgct gggagacaga agacggccag aaacgtagca
     1381 agcacgaagt cgtggcgcaa ggcgtgacct ttttgccaaa gcggggcgaa gccggagccg
     1441 gagcagacag cggcggcctg cacgacgaac caccttcata cgaagacgaa cagttctagg
     1501 agacggaggc gatcatggaa cgaggagatc gtggaaacag cggagggggc ggaggccggt
     1561 tttttcaacg ccgcaagcct tgccggtttt gtgtggataa ggcaccgatt gatttcaagg
     1621 atgttggcct gttgcggaat tttttgaccg agcgtggacg tatcgttccc cgtcgcattt
     1681 ccggcaattg cttgcagcat cagcgggtgt tgacggtcgc agtgaagcgg gctcgccaga
     1741 tcgccctggt gagttttgcc gaagagcgat agggcaggga taggttttgg tgagtgttga
     1801 gtcacgtggc ccggcagcct gtctcctgat cgatacgggg gaggatcgtc tcgtcatgac
     1861 ggtgcccatg gatatgctca acccagcgat tgtcgatatt ggtccggaag ggctgcccct
     1921 gtcatgctcg gcttcggggc ctcagctggg gttggatgag ccggaagata agtttgacgg
     1981 cccgctggcc attcagcttg agctctcgca gcaagaagga cggatctcgg tcaccggcac
     2041 gttggaaggc accgcgatcc gtcaatgcgt gcgttgtctt tcggaatatg ccgatccgct
     2101 cctcgtctct ctctatgcgg aatatcttcc gcaggcgagt gtggctgcga aatctgctcc
     2161 ggcggaacag ggaagacgga atacgagacg cggtgctcaa ccggttgaac cggaagaaga
     2221 ggcggatgaa gaggatgaac tgtactggta tcagggcgat catcttgatc tcgtcccgat
     2281 gctgcgcgaa caggtaattc tggccgctcc gatgcagccg atatgtcgcg aggattgccc
     2341 gggactttgt ccccagtgcg gacagaacct taatgagcgc cgatgcgact gccctccggc
     2401 gcaagtgccg agtccgtttc gggtgctgcg agggcgtggg ccaaaaggtg aaaacgcata
     2461 gtctgttaac cgggtgttgt gcccgttgaa ggagtcgtca tgccaaatcc aaaacacaaa
     2521 cattcacgat cccgtcgcga catgcggcga acagcgaaga cccggctggt gcctccagga
     2581 ttttcgctct gcccgcagtg ccacgaattg aagctcccgc actatacgtg catgaattgc
     2641 ggaacgtaca aaggcaaggc cgtcatcgtc gtcgaggaat cctagtcgcg ttttattttt
     2701 tcgacgtgat tcgggtacaa ttgcccaccg tgatcacgat ccgacgctat gtcatgcgtg
     2761 aggccgttca ggtcctagcc ggcagggcgt ctctgcacga ctcttcactt ctctatccgt
     2821 ggaccgctaa ccgcacatga agattgccgt cgatgcgatg ggcggagacc acggaccggc
     2881 cccggtcatt g
//
LOCUS       DAOQEW010000340         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17633_length_2887_cov_6.155014, whole genome shotgun sequence.
ACCESSION   DAOQEW010000340 DAOQEW010000000
VERSION     DAOQEW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17633_length_2887_cov_6.155014"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..356
                     /locus_tag="PLS35_19690"
     CDS             195..356
                     /locus_tag="PLS35_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20950.1"
                     /translation="MSVAYRQYGYRLDEIAMHRGVHYATISRCMKQAEQETNDSNDPA
                     PPGMCDCKT"
     gene            384..983
                     /locus_tag="PLS35_19695"
     CDS             384..983
                     /locus_tag="PLS35_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20951.1"
                     /translation="MTIKLPAIIPAIVLMSVVLSGCPLPRSEMYKGLSEAEKSYFATA
                     DLGVFPNDARKDVERYQASLVAWPGFITELNSIQKDSAYFTRIVVEHHYYDWILDHSI
                     QKEKYFLSPRGEGVFTTLCPSKLKDENVTKDLNNMLIVYGKPEKVEDGMLHVTCDYAA
                     FIKREWVRTDIFDYGRPGESVKWLNTPGPGAGTVLRKSK"
     gene            complement(1377..2240)
                     /locus_tag="PLS35_19700"
     CDS             complement(1377..2240)
                     /locus_tag="PLS35_19700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNI20952.1"
                     /translation="MNTLLAVDGSDHAYEAVRALKYLRRADQLTLLHVVDAPRPAYPM
                     MVPEVAQELYAQLERSMKEDGEELLTRIQSLLPLHSGPVTKLLQVGSPAEHIVAAAQS
                     CQADLIVMGARGLGPVKERLLGSVSHRVLSMAPCAKFVLHGSLRQLKHILLPLQGPSD
                     AEAALRFLAKQPFQEPVNVSVLTVLPPTRPPWPIDDTAAEKLEAHALRHARDFVEDVA
                     ATLRTQGYTTRSASVLGSPLTMILHEAEKLGADLIMVGSRARQGITRFVLGSVSHAVL
                     HRAPCQVLVFE"
     gene            complement(2237..2887)
                     /locus_tag="PLS35_19705"
     CDS             complement(2237..2887)
                     /locus_tag="PLS35_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNI20953.1"
                     /translation="MTITGDGIALDGILGLPGQPKGVIAFAHGSGSGRFSPRNQFVAR
                     VLETGGFATLLLDLLSPDEADDRRKIFDIDLLADRLLLAQHWLQTHASTRTLRTGYFG
                     ASTGAGAALQAAAREPDAVGAVVSRGGRPDLAGPYLSRVIAPTLLLVGGEDGPVIGMN
                     EEALGQLTCQKRLIIIPGATHLFEEPGTLEQVAQEALNWFRRYLHPAPYQPKELLP"
BASE COUNT          645 a          824 c          850 g          568 t
ORIGIN      
        1 gatctggcga accgcctcgt cacgagcctc acgctgtcga actgggcgca gacgacctac
       61 agtgacgatc cggcccgtca ggtgaccagc atcctgcatc aacgctgaat gagggttctt
      121 agcgacgagg agtggtaagc cacacttcgg ccaaggcttt ctctaaactc cgaccgggcg
      181 aggggacagg gctcgtgtcg gtggcgtacc gccagtatgg ttatcggtta gacgaaattg
      241 ccatgcatcg cggcgtgcac tatgcgacga tcagtcgctg catgaagcaa gccgagcagg
      301 aaacaaacga tagcaacgac ccggctcctc cgggcatgtg tgattgcaag acctgacccc
      361 ttttggatta gtttgagata ccaatgacga taaagctgcc cgccattatt ccggccatcg
      421 tactcatgtc cgtggttcta tccggctgcc cattacccag atcagaaatg tataagggcc
      481 tctccgaggc ggaaaagtca tattttgcaa cagccgacct cggtgtcttt ccgaatgatg
      541 cgagaaaaga tgtcgaacga tatcaggcta gcctggtggc atggccgggc ttcataacag
      601 agctgaattc gatacagaaa gacagcgcat acttcacgag aattgtcgtg gagcatcact
      661 attacgattg gatactggac catagcattc aaaaagaaaa atatttcctt tctccgcgag
      721 gggagggggt gttcacaaca ctttgtccgt ccaagttgaa agacgagaat gtcactaagg
      781 atttaaacaa tatgctcatc gtttatggaa aaccggaaaa agtggaagat gggatgctac
      841 acgtgacttg tgattacgcg gcattcataa agagggaatg ggtgagaaca gatattttcg
      901 actacggtcg tcccggtgag tctgtaaagt ggctcaatac acccgggcct ggagccggaa
      961 ccgtcctgag gaaatctaag taagtgtggg agcattcgaa gggatacgga acgcttaggg
     1021 cttccatgat gaccagtgga gcatatgccc ccacccagat aggggcaggt cttgcaatca
     1081 tacatgtgag ttgctcacgt ctcgcccatg gcccgttcgt tgcgcataga gtttcccgct
     1141 gccgtctatc atgtgaccag tcgtggcaat gccacgcagg atattgtagt tgaccatcag
     1201 gatcgcacga gcatcctgag ctgctcgcac acatgattca ccattgcaag acctaaccct
     1261 ccaactccgg actccctaag tcgtcggaga gaccctagaa ttaagtcgca cagtacaccg
     1321 atacgacggc aacgacgatc tcaccaaacg ccgcgcgatc gagggcggcg actctatcac
     1381 tcgaacacca gcacttggca tggggcccgg tggagtacgg cgtgagagac gctccccagg
     1441 acgaagcggg tgatgccctg tctggcgcgt gagccgacca taatcagatc tgcccccaat
     1501 ttttccgcct cgtgcagaat catggtgagc ggggaaccca gcacactcgc actgcgggtt
     1561 gtatagccct gtgtgcgaag ggtcgccgcc acatcctcca cgaagtcgcg ggcgtgacgc
     1621 agcgcgtgcg cctctaattt ttcagccgcg gtatcgtcta ttggccatgg gggacgtgtg
     1681 ggcggaagga cggtcaggac gctcacattc accggttcct gaaagggctg tttggcgaga
     1741 aaccggagtg ccgcctcggc gtcggacggt ccctgcaacg ggaggaggat gtgcttcaac
     1801 tggcgcaatg atccgtgaag aacgaatttg gcgcacggag ccatactcaa tacgcgatga
     1861 gagacgctgc cgagcagccg ttccttcacc gggcccaggc ctctcgcccc catgacgatg
     1921 agatccgcct ggcaggattg agccgcagcc acgatatgct ccgccggtga tcccacctgc
     1981 agcagtttgg tgaccggccc gctgtgaagg ggaaggagcg actgaatccg cgtcaacaat
     2041 tcctcgccgt cctctttcat gctgcgttcc aactgagcat acagttcctg cgccacttcc
     2101 ggcaccatca tagggtaagc cggacgcggg gcatcaacca cgtgcagaag tgtgagctga
     2161 tccgcgcggc gcagatactt cagcgccctc acggcttcat acgcatggtc ggatccatcg
     2221 actgcgagca acgtgttcat ggcaggagct cctttggttg gtacggtgcc ggatggagat
     2281 accgacgaaa ccagttgagg gcctcttggg cgacctgctc aagtgtgccc ggttcttcga
     2341 agagatgcgt ggcccccggg ataatgatca atcgtttctg acaagtcagc tgcccgagtg
     2401 cctcctcatt catgccgatc accggaccat cctcgccacc cacgagcaac aaggtcggcg
     2461 cgatgacgcg gctcaggtac ggaccggcga gatcggggcg cccgccgcgc gagacgacgg
     2521 cacccaccgc atcgggctcg cgcgcggccg cttggagggc agctccggct cctgtgctgg
     2581 ccccgaaata tcccgtgcga agagttcgcg tcgaggcatg ggtctgcagc cagtgctgtg
     2641 ctaggagcaa gcggtcagcc aggaggtcga tgtcgaaaat cttccgccga tcgtcggcct
     2701 catcgggcga cagcagatcc aggagcaggg tggcgaatcc gcctgtctcc aggacgcggg
     2761 cgacgaactg attgcggggg ctgaagcgac cgctgccgct gccatgggca aacgcaatga
     2821 cccccttcgg ctgccccgga agtccgagga ttccgtcgag ggcgattcca tccccggtga
     2881 tcgtgat
//
LOCUS       DAOQEW010000341         2860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_17946_length_2860_cov_10.221747, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000341 DAOQEW010000000
VERSION     DAOQEW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17946_length_2860_cov_10.221747"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="ispE"
                     /locus_tag="PLS35_19710"
     CDS             complement(<1..799)
                     /gene="ispE"
                     /locus_tag="PLS35_19710"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNI20954.1"
                     /translation="MNSTSLLVLTPAKVNLILRVLERRPDGFHAIWSLMHTVGLNDEL
                     MLTMQPAAASRITLRCNQADLAVDQTNLVYRAAELVLARANRTMDLSISLTKRIPMGA
                     GLGGGSSDAAATILGLTRLLELNWSVEQMAEVGQQLGSDVPFFFVAPAACVTGRGEHV
                     RPVRVTGNRWIVLVSPGFPVPTKWAYQQLAATRAGVRPLSVAVQSLDQRSELDWSEIV
                     PLVENDFEEPVFAHHPVLAQIKSQLLSLGAEVALLSGSGATMFGLFRD"
     gene            complement(820..2199)
                     /locus_tag="PLS35_19715"
     CDS             complement(820..2199)
                     /locus_tag="PLS35_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNI20955.1"
                     /translation="MEKILVVDDEQGLRDVLSIMLKRAGYAVTVASDGEEAIAQIHKE
                     IFDLVITDLKMPKMGGLDVLKAVKASSPDTVVLMITAFASADSAVEAMKHGAYDYLTK
                     PFQVDEVQLIIRNAIERRRLSTENMLLKRELASQSSFSQIVGQSEAMQKVYDVIKKVA
                     DSRSNVLIGGESGTGKELVARAIHFNSARASMPFVTVNCSAVPETLLESELFGHMKGS
                     FTGAVSNKAGLFEVANGGTIFLDEIGDTTPAIQVKLLRVIQEREFRRVGGTQDVKVDV
                     RIVAATNRDLEKAVAEGAFREDLYYRLDVIPIRLPPLRMRTGDIPLLSQHFLEKFAKE
                     SGKPVPTMSQEAMRVLLAHEWRGNVRELENVVERVVAFTTGSSVTDADIRGWLHKPVS
                     TQQAVPSELPEDGLDLEGLINTIEKDLLLKALERSQWVKKRAARLLRLNTRSFRYRLE
                     KYEIKGGRD"
     gene            complement(2184..>2860)
                     /locus_tag="PLS35_19720"
     CDS             complement(2184..>2860)
                     /locus_tag="PLS35_19720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20956.1"
                     /translation="GALAGAMQMLRQDVGSDETSQKLMDIAIREARRLDNIITEFLQY
                     ARPPALNLAEQDLNKVLADTLDLIQHEARSRTGITIVSRPTPIALRAKVDQDQLKQVF
                     WNLSVNAFDAMPGGGTLTITTGLRRVEVGGRREDVIEISVHDSGEGIPKQNFDKIFLP
                     FFTTKKEGSGLGLAQVHRIVELHDGWIKVESEVGTGARFVVCLPQSPEVGVRLRHEGR
                     EPWKRF"
BASE COUNT          595 a          930 c          753 g          582 t
ORIGIN      
        1 ggtctcggaa caggccgaac atggtcgctc cgctcccgga taacagggcc acctcggcgc
       61 ccaagctcag caattggctc ttaatctgcg ccagcaccgg atggtgagcg aacacgggct
      121 cctcgaaatc gttttctacc aacggaacaa tctccgacca atccagctcg gagcgttgat
      181 ccaaggactg caccgccacc gagagcggac gcacaccggc gcgggtggca gccaactgct
      241 gatacgccca tttggtcggc acaggaaatc ccggactgac cagcacgatc catctgtttc
      301 ccgtgactcg gaccggacgg acatgctctc cccgtcccgt cacacaggcg gcaggcgcca
      361 caaaaaagaa cggcacatca cttcctaact gctgccccac ctccgccatc tgctcgaccg
      421 accagttcaa ttccaacaac cgggtcaacc cgagaatggt tgcggcggca tcactgcttc
      481 ctccaccaag tccggccccc atgggaatgc gctttgtcag gctgatggag agatccatcg
      541 tccggttggc tcgcgccaac accagctccg cggcacgata gaccagattg gtctgatcca
      601 ccgccaggtc ggcctggtta cagcgcaagg tgatgcggga ggcggcagcg ggctgcatgg
      661 tgagcatcaa ctcatcgttg agccccaccg tatgcatcaa tgaccagatg gcatggaaac
      721 cgtcaggccg tcgttcgagc acccggagaa tgagattgac cttggcagga gtcagaacca
      781 gcagactagt agagttcact cgcagctaac agggcggcgc taatcccggc ctcctttgat
      841 ttcatacttt tcgagacggt accggaacga ccgggtattc aaccgcagca gtcgcgcggc
      901 tcgctttttg acccactgac tccgctccag cgctttcaac agcagatcct tctcgatcgt
      961 gttgatcaac ccctccagat ccaggccatc ttccggcaat tccgagggca cagcctgctg
     1021 ggtgctcact ggcttatgga gccatccgcg aatatccgca tccgtcaccg acgatcccgt
     1081 ggtgaaggcc acaacgcgtt cgacgacgtt ctccaattca cgaacgttgc cccgccactc
     1141 atgcgccaac agcacgcgca tggcctcctg gctcatcgtc ggcactggtt taccactttc
     1201 cttggcaaat ttctcgagaa aatgttggct cagtagcgga atgtcgccgg ttcgcatgcg
     1261 caatggcggg agcctgatcg gaatcacatc gagccgatag tagagatcct cacgaaatgc
     1321 gccttcggca accgcctttt ccaagtcacg attcgtggcc gccacgatac gcacatccac
     1381 tttcacatcc tgcgtgccgc cgacccgccg gaactcccgc tcctgaatga cgcgcaagag
     1441 cttgacctga atcgcaggcg tggtatcgcc gatctcgtcg aggaaaatcg tgccgccgtt
     1501 cgccacctca aacagaccgg ccttgttgga aaccgcgccg gtgaaggagc ccttcatgtg
     1561 gccgaacagt tcactttcga gcagcgtttc tggcaccgcg ctgcaattga ccgtcacgaa
     1621 cggcatggag gctcgcgcac tgttgaaatg gatggctcgc gccaccaact ccttgccggt
     1681 gccgctttcc ccgccgatca ggacgttgct cctggaatcc gccactttct tgatgacatc
     1741 gtagaccttc tgcatggcct cgctctggcc cacgatctgg gagaacgacg actggctggc
     1801 caattcccgc ttcaggagca tgttctccgt cgagagccgg cgtcgctcga tcgcgttgcg
     1861 aatgatcagc tgcacttcat cgacctggaa aggcttggtg agatagtcat acgcgccgtg
     1921 cttcatggct tcgactgcgg aatcggcgga ggcaaaggcc gtgatcatca acacaacggt
     1981 gtcaggcgat gaggccttga cggccttgag cacgtccagg ccacccattt tcggcatctt
     2041 gaggtcggtg atgaccagat caaaaatttc tttgtgaatc tgcgcgatcg cctcttcccc
     2101 gtccgacgcc acggttacgg cgtaccccgc ccgcttgagc atgatgctca acacatcgcg
     2161 cagaccctgc tcatcgtcga caactagaat cttttccacg gttcccttcc ctcatgccgc
     2221 aaacgcacgc cgacctccgg ggactgcggg aggcagacga caaaacgcgc cccggtgccc
     2281 acttcgctct caactttgat ccatccgtcg tgcaactcca cgatgcgatg gacctgggcc
     2341 agacccaacc cggacccctc tttcttcgtc gtgaaaaatg gaagaaaaat cttgtcgaag
     2401 ttctgctttg gaatgccttc gccgctatcg tgaacgctga tctcaatcac atcctcgcgg
     2461 cgtcctccga cttccacgcg gcgcagtccg gtagtaatcg tgagggttcc tcctccgggc
     2521 atggcgtcaa acgcattgac ggacaaattc cagaacacct gcttcaactg atcctggtca
     2581 acctttgccc ggagagcaat cggggtcgga cgagacacga tcgtgatgcc ggttcgtgat
     2641 cgcgcttcat gttggatcag atccaacgta tcggcaagga ctttattcaa atcctgctca
     2701 gccagattca aggccggcgg gcgcgcatac tgtagaaact ccgtgatgat attgtcgagc
     2761 cgcctggctt cccggatggc gatgtccatc aatttctggc tggtctcgtc ggagccgaca
     2821 tcctgccgca acatctgcat cgcgccggcc agcgcaccca
//
LOCUS       DAOQEW010000342         2791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_18729_length_2791_cov_7.883041, whole genome shotgun sequence.
ACCESSION   DAOQEW010000342 DAOQEW010000000
VERSION     DAOQEW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18729_length_2791_cov_7.883041"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="PLS35_19725"
     CDS             <1..1498
                     /locus_tag="PLS35_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HNI20957.1"
                     /translation="GPKIMVKDIRLVGNTAFTSQQLSEITAPYTNRELTAEDLESLRL
                     ALTYYYVNHGYLTSGAVVPEQDVADGTLTMQIIEGKITQVNVEDTKWFRPSYFQSRVN
                     LAAGPPLQVEALQERLRVMQANPRIERINAELLPGATIGENTLNVKVKEANPLKAWLE
                     FNNYQSPVVGAEQGFITLAHQNLLGFGDTLSLQYGRSAGVNPMLNFKYEIPVGPRDTT
                     VALQYRRFDFGVEESPFDSLDIKNKAQIFGLSVRHPVIRKADQELAFSLTGEHARNES
                     TLGGNPFELITGAPNGRFRVTALRFGQEYAQRSAEQVISLLSRFSVGIGAMGATANAD
                     PNLPDARFFSWLGEAQWIRQLPFWRTQLVSRGVVQLSNDHLFPLEQIAVGGRYSVRGY
                     REFTLIRDNAAMASIEARVPVYTTKAGVDSVFLAPFFDFGHGWQTTAQTPGTPPKTLA
                     SLGAGVIWNFWRGSHFELYYGKQLKRYDTDRNNLQDHGIHLQLVVEAF"
     gene            1777..>2791
                     /locus_tag="PLS35_19730"
     CDS             1777..>2791
                     /locus_tag="PLS35_19730"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HNI20958.1"
                     /translation="MLCRPAGLPLLVRGYLAFLMLLGSTASIAYGQATNIVATPGGPG
                     GLGTTLNTAGNTTNITGGTRPGGGPNLFHSFNQFSVGAGDVASFVNPGGVSNVISRVI
                     GGSPSNINGTVQALNANLFFLNPSGIIFGPSAHLNVSGSAYFSTAQQLRLSDGGIFTA
                     STSLLAFDSTLSASSPVAFGFLGQGPYGSIVLTSSSTVLQTGAVLGLMGGGIQINGSK
                     ISAQRVMLGSTSSAGEMSTQPFVGNPFSGAAGNGQVQALPGTLIVAGGGGVIPASIDV
                     SPNVTVPTGAQVNTTSNSVTQRVTQIQVVGVNLGGLPPLPTANAANVNPTNPVEPVAF
                     LNRA"
BASE COUNT          571 a          787 c          799 g          634 t
ORIGIN      
        1 gggacccaag atcatggtca aggatatccg gctggtcggc aataccgcct ttacgtcgca
       61 acaactctcg gaaatcaccg cgccatatac gaatcgcgaa ttgacggcgg aagacctgga
      121 atcgttgcgt cttgcgctca cctactacta cgtcaaccat ggctatttga cgtccggtgc
      181 ggtggttccg gagcaggatg tcgccgacgg caccctgacc atgcaaatca tcgagggaaa
      241 gattacgcag gtcaacgtcg aggataccaa gtggttccgg ccttcctatt ttcagagccg
      301 cgtcaatctg gcggccggcc ctcctctcca ggtcgaagcg ctccaggaac ggttgcgcgt
      361 gatgcaagcc aatccccgga tcgaacggat caatgccgaa ttgttgccgg gcgccacgat
      421 tggagaaaat acgctcaatg tgaaggtgaa ggaagccaac ccgctcaagg cctggttgga
      481 gttcaacaac tatcagtctc ccgtcgtcgg tgcggaacaa ggcttcatca cgttagctca
      541 ccagaacctg ttgggcttcg gcgatacgct gagtttgcaa tacggtcgct ccgccggcgt
      601 caacccgatg ctcaacttta aatatgaaat cccggtaggc cctcgcgata cgaccgttgc
      661 actgcagtat cgccggtttg atttcggcgt cgaggagtcg ccgttcgatt cattggatat
      721 caagaacaag gctcagatct ttggcctctc ggtcaggcac cccgtgatca ggaaggctga
      781 ccaggagctg gcgttttccc tcacgggcga acatgccagg aatgagagca cgttgggcgg
      841 caatccgttt gagctcatca ccggggcgcc gaacggaaga ttccgtgtga ccgcgctccg
      901 tttcgggcag gagtatgccc aacgttccgc cgaacaggtg atttcccttt tgtcgcggtt
      961 ttccgtcggt atcggagcga tgggggcgac cgccaatgct gatccgaacc tgccggatgc
     1021 gcggttcttt tcctggttgg gcgaagccca gtggatccgg caacttccgt tttggcgtac
     1081 gcagttagtg agccgcgggg tggtgcaatt gtccaacgat cacctcttcc cgcttgaaca
     1141 aattgccgtc ggcggacggt atagcgtccg cgggtaccgc gagttcacgc tgattcggga
     1201 taatgccgca atggcgtcga tcgaagcacg agtgccggtc tatacgacaa aggcgggtgt
     1261 ggactccgtc tttctggcac cctttttcga tttcggccat ggctggcaaa ccactgccca
     1321 gactcccggt actcccccca aaacattggc cagtcttggt gctggcgtga tctggaattt
     1381 ttggcgcggg agtcattttg aactctatta tggaaaacag ttgaagcgat acgataccga
     1441 tcgcaacaac ctccaggatc acggcatcca tctgcaattg gtcgtagagg cattttagcg
     1501 agagggacgg cttcgatgga atcgcgcctg tctcgctgag gcagcatgtt gtccttgagg
     1561 gtaaataacc ccctccacgt tgacggcgtt tttttgccgt tggttgtgca cgtgagccac
     1621 aggcgactca tgcactttgt tagggctgaa tatccagggc cgattggcca ggtctcggcc
     1681 cttgtactcg cgggtccgga cagggtgtgc gtgttgaagt tgtgtgtcgt ttaaggtaag
     1741 agaggtggcc tctcttacca catgaggaga ttgaccatgc tatgtcgccc cgcaggattg
     1801 ccgctgttgg ttcgtggata tctcgccttc ctgatgctcc tcggctctac cgcatcgatc
     1861 gcttacggac aggccaccaa catcgtcgca actccaggcg gaccgggcgg tcttggaacc
     1921 acgctgaaca cggcaggcaa tacgaccaac attaccgggg ggacgagacc gggaggcggt
     1981 cccaatctct ttcatagctt caatcaattc tcggtcggag cgggagatgt ggcgtcattc
     2041 gtgaatcctg gtggcgtgtc gaacgtcatc agtcgagtga tcggtggatc accgtccaac
     2101 atcaacggca cggtgcaagc cctcaatgcg aatctattct ttctgaatcc ttccggaatc
     2161 atattcggcc catcggccca cctgaacgta tccggatctg cgtatttcag caccgctcag
     2221 caactacgtc taagcgatgg aggcatcttt accgcgagca ccagcctgct ggctttcgat
     2281 tcaaccttgt ccgccagttc accagtggcg ttcggatttt taggccaggg cccttacggg
     2341 tccatcgtgc tgacctcttc ttcgacagtc ctccagaccg gcgccgtctt gggtctcatg
     2401 ggaggaggca ttcagatcaa cggatcaaag atcagcgctc aacgggtcat gctcggcagt
     2461 acgagttcag cgggggaaat gagtacccag ccatttgttg gaaatccctt ttccggggcg
     2521 gctggtaatg gccaagtgca agcgttaccg ggaacgttga tcgtcgcggg cggtggtgga
     2581 gtcattcccg cctcaattga cgtgagtccg aatgtcaccg tgccgacggg tgcacaggtc
     2641 aacacgacga gcaattcggt cacgcaacgt gtgacgcaaa tccaagtggt cggcgtgaat
     2701 cttggcggcc ttccgcctct ccctaccgca aatgcagcta acgtcaatcc gacgaatccc
     2761 gtcgaacctg tcgcgttctt gaatcgtgcc g
//
LOCUS       DAOQEW010000343         2781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_18840_length_2781_cov_7.991196, whole genome shotgun sequence.
ACCESSION   DAOQEW010000343 DAOQEW010000000
VERSION     DAOQEW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18840_length_2781_cov_7.991196"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLS35_19735"
     CDS             complement(<1..1322)
                     /locus_tag="PLS35_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNI20959.1"
                     /translation="MALFREYDLRGIVGEELTEPIAEQVGRAYATMAREQGASRISVG
                     RDGRLSSPALQQALLRGLLAGGLDVVDLGLCASPLLYFSLFTLPVHGGIMITGSHNAA
                     EYNGFKICIGKEAIHGEEIQQLRSIMERGRFSSGSGQLSSHPIIPDYLQHLKTVFAGV
                     RADHLHVVIDCGNGAASLVAKQALEQMGCRVTGLYDELDGRFPNHHPDPTVVENLQDL
                     IATVREHKADVGIGYDGDADRIGAIDERGEILWGDRLMIVYARDILAQRPGTTFISEV
                     KASQCLYDDIAAKGGRPIMWKTGHSLMKAKLKAESAVLAGEMSGHMFFADRYFGFDDA
                     IYASCRLVEILAKTKHSLSSLVADLPQTTVTPEIRVDCPDTVKFQLVEQVHRQLASYL
                     EAGQPVGASNLRMRELVTIDGVRAIFEDGWGLIRASNTQPALVLRFEAP"
     gene            complement(1331..>2781)
                     /locus_tag="PLS35_19740"
     CDS             complement(1331..>2781)
                     /locus_tag="PLS35_19740"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HNI20960.1"
                     /translation="MTNHLYPVILAGGSGTRFWPLSRHLYPKQLLRIIGDETLIQQTM
                     RRVLSCASPDRVLISTNPGQADSIRVQLGEWKSDLKDNYVIEPIGRNTAPAIALVAAE
                     LLRRDPEAMMLVLPADHVVTGEKAFQAAVTLGMQLAEKGHLVTFGIKPTRPETGYGYI
                     QPNRRTTLAKKDRLTGHPVARFVEKPSRTKAAQYLKSGNYFWNSGMFLWKAATIRDEI
                     NRHQPQLAKAVQKVHTLMTSGADPRQIEAAYKKVPSVSIDNGVMELSSHAAMIPVGFG
                     WSDVGNWSSLEEVAPRDKAGNVVSGRVIDMDSSNSVLYADRRVVATIGLTDMVVVDTP
                     DATLICPKSRSQDVKQMVEILKQQGAPEHLEHVTVFRPWGSYTVLEEGPGYKVKRVTV
                     NPGGRLSLQLHHKRSEHWVVITGTARVTRGDDVLHLQIGQSTAIPVETPHRLENPGTE
                     TLHIIEVQNGPYLGEDDIVRFQDDYGRLGRAR"
BASE COUNT          566 a          870 c          769 g          576 t
ORIGIN      
        1 ggcgcctcaa accggaggac caatgccggt tgggtgttcg aggcgcggat caacccccat
       61 ccatcctcga aaatcgcgcg aacgccgtcg atcgtcacca attcccgcat ccggagattc
      121 gacgcgccga ccggctggcc ggcctcgaga taggaggcta actgccgatg cacctgttcg
      181 accaattgaa acttcacggt gtcgggacaa tccactcgaa tttccggtgt cactgtagtc
      241 tgcgggagat cggccaccag actcgagagc gagtgtttgg tctttgccag aatctcgact
      301 aaccggcagg aagcgtagat cgcgtcgtca aacccgaagt accgatcggc gaaaaacata
      361 tggccggaca tttcgcctgc gaggacggct gattcagcct ttaatttcgc cttcatcaaa
      421 gaatggccgg ttttccacat gatcggacgt ccgcctttgg ccgcgatatc gtcgtacaag
      481 cattgcgagg ccttgacctc agaaatgaag gtagtaccgg gcctctgagc gagaatatcg
      541 cgcgcgtaca cgatcatgag gcgatccccc cagaggatct ctccccgctc gtcgatcgcg
      601 ccgatgcgat cggcatctcc gtcgtacccg atcccgacat ccgccttgtg ttcccgtacg
      661 gtggcaatca gatcttgaag attctccacg acggtaggat cgggatggtg gttcggaaaa
      721 cgcccgtcca actcatcata gagcccggtc acgcgacagc ccatctgctc cagcgcctgc
      781 ttggcgacta gcgaggccgc gccattgccg caatcgatga cgacatgcag gtgatctgcc
      841 cggacacccg cgaacaccgt cttgagatgc tgtagatagt ccggaatgat gggatgagag
      901 gacagttgcc cggagccgga actgaatcgg ccgcgctcca tgatgctccg caactgctga
      961 atctcttcgc cgtggatcgc ttccttcccg atgcagatct tgaagccgtt gtattcggcg
     1021 gcgttgtggc tgccggtgat catgattccg ccgtggaccg gaagcgtgaa caaggaaaaa
     1081 tacaacagcg gcgatgcaca gaggcccaaa tcgaccacat cgagcccgcc ggcaaggaga
     1141 ccccgaagta aggcctgctg caacgcaggc gagctcaggc gcccatcccg ccccacactg
     1201 atccgcgagg ccccttgctc cctggccata gtggcatagg cccggcccac ctgctccgca
     1261 atgggttccg taagctcttc tccgacaatg cctctgagat catattcgcg aaacagcgcc
     1321 atgccatctc ctaacgtgcc cggccgagtc gcccgtaatc gtcctgaaac cgcacgatgt
     1381 catcttcacc gagatagggc ccgttctgca cctcgataat gtggagcgtc tcggtacctg
     1441 gattttcgag gcgatgggga gtttcgaccg gaattgcggt gctctgcccg atctggagat
     1501 gcagcacgtc gtcgccgcgg gtcactcgag cagtccctgt gatcaccacc cagtgctcgc
     1561 tccgcttatg gtgaagctgc aatgacaatc ggccgcccgg attgaccgtc acacgcttca
     1621 ctttatatcc aggcccttct tccaagaccg tataggaacc ccacgggcga aacaccgtca
     1681 catgttccag atgctcaggc gccccctgtt gcttgaggat ctctaccatc tgcttcacgt
     1741 cttgcgagcg ggatttcgga cagatcaagg ttgcatccgg cgtatcgacg acgaccatat
     1801 ccgtgaggcc aatcgtggcg acgacccggc gatcggcgta aaggacggaa ttgctgctgt
     1861 ccatatcgat aactcgaccg ctgaccacat taccggcctt gtcgcgcggc gcaacttctt
     1921 caaggctact ccagttgccc acatcggacc agccgaaccc caccggaatc atggccgcat
     1981 gcgacgataa ctccatcacg ccgttgtcga tcgagaccga aggaactttc ttgtaggcgg
     2041 cttcaatctg ccggggatcg gcgcccgatg tcatcagggt gtgaactttc tgcaccgctt
     2101 tcgccagctg cggctggtga cgattgatct cgtctcgaat cgtggcggct ttccagagga
     2161 acatcccgct gttccagaag taattgccac ttttgagata ctgcgccgcc ttggtccgac
     2221 tcggtttctc gacaaatctg gcaaccgggt gacctgtcag ccggtccttc ttggctaagg
     2281 tagttcgccg gttcggttga atgtatccat acccggtttc cggcctggtc ggcttgatcc
     2341 cgaaggtcac caagtgtccc ttctcggcaa gctgcatccc caacgtcact gctgcctgaa
     2401 acgccttctc cccggtgacg acatgatctg ccggcaacac cagcatcatc gcctcgggat
     2461 cgcgtcgaag gagctccgcc gccaccagcg caatggccgg cgcggtgttg cgccctatcg
     2521 gctcaatgac atagttgtcc ttgagatcgg acttccattc ccctaattgc acgcgaatcg
     2581 agtcggcctg ccctggattg gtcgagatga gcacccgatc ggggctggca caggagagca
     2641 cgcgccgcat ggtctgctga atgagggtct cgtcgccgat gatgcgcagg agctgcttag
     2701 gatacaggtg ccggctcaat ggccaaaacc tggtcccgct gccgcccgcc agaataaccg
     2761 gatagaggtg atttgtcata t
//
LOCUS       DAOQEW010000344         2771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_18956_length_2771_cov_7.882180, whole genome shotgun sequence.
ACCESSION   DAOQEW010000344 DAOQEW010000000
VERSION     DAOQEW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18956_length_2771_cov_7.882180"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..211)
                     /gene="rpmB"
                     /locus_tag="PLS35_19745"
     CDS             complement(20..211)
                     /gene="rpmB"
                     /locus_tag="PLS35_19745"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNI20961.1"
                     /translation="MAFACDLCGKKHQTGNNVSHANNKTKRVFNPNLQRVKALVNGSA
                     LRIRVCTRCLRSGLVKKAV"
     gene            372..629
                     /locus_tag="PLS35_19750"
     CDS             372..629
                     /locus_tag="PLS35_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNI20962.1"
                     /translation="MDKVTFIGLLAGTLTTIAFIPQLQQTWRTRSAQDVSLGMLLTFI
                     IGVFLWLVYGLLLGALPIILANLVTLVLTLAILVLKLRYRA"
     regulatory      677..864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1072..1971
                     /locus_tag="PLS35_19755"
     CDS             1072..1971
                     /locus_tag="PLS35_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNI20963.1"
                     /translation="MKRRQQGILTGMPFMANITPRTFIDDAGRKLYVAKAPTRVVSLA
                     PSITEMLFALGLDEQVVGVTDFCNFPAAAAAKPKVGYSNPNLESLIALRPELIVAPLE
                     FHRANVLAKLEELKIPVLLLEATSLEQVFAHIHTLGRIFDRSTAAHAMTAEMRQQMAE
                     ISRRTEGLARTRVLYVINSQPLITVGPGSYIHQMIGLAGGTNIAAAAATAYPRLTMET
                     VLKEDPEILIFPRGSVETVPRSEQEAWRRWTTLTAVQQNRLREVSADALNRPGPRVMQ
                     GLEALARVIHPEAFDSEPMTIKP"
     gene            1968..>2771
                     /locus_tag="PLS35_19760"
     CDS             1968..>2771
                     /locus_tag="PLS35_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNI20964.1"
                     /translation="MMPSAGHPTSPPPALTSPSLSPNLMQSGPSFTATDRPFQGAILT
                     RSRWCAVMGALLLLSVILAMLCLQLGTQYIGMGHILTLVWSAVVGTSNEPDALRTTGV
                     ILLQVRLPRVLLGFLVGSCLAAVGVSLQALLRNPLADPYVLGVSSGAALGVAIAVLFG
                     IGTTVLALSLLPVCGFAGSLVALLVIYRMAASYERLPIHSVLLAGVILNAIFSALIMF
                     ITSIMEPNRSFGMMAWLMGSLTAPAYPVLIALSVYLLVGLVLLGKHVRVL"
BASE COUNT          503 a          806 c          824 g          638 t
ORIGIN      
        1 aagggaagcg cgggagagat cagaccgctt ttttaaccag acccgaccgc aggcagcgcg
       61 tgcacacgcg aattcgcaaa gccgacccgt tcaccagtgc cttcacgcgt tgcaaattag
      121 gattgaagac gcgcttcgtc ttattgtttg cgtggctgac gttgttaccg gtctgatgct
      181 ttttcccaca gagatcacag gcaaatgcca cggccctact cctttgttgc ggcgtctttc
      241 aacgactatc cttgaatggg aagggatgct accatagcgc caggggacaa gacaagctgc
      301 cggggtgaat tctcctttca ggataccctc ttccgctcac atcgcacgtc attccatctg
      361 aggacttccc tatggataag gtcacgttta tcggattact cgcaggaacc ctgacgacaa
      421 ttgccttcat ccctcaactc cagcagacct ggcgcacgcg ctccgcgcag gatgtgtcgt
      481 tgggcatgtt gctgacgttc atcatcgggg tgtttctgtg gttggtctac ggactgctgc
      541 tcggcgcgct gccgattatc ctggcgaatc tcgtgacctt ggtgctgacg ctggccattc
      601 tcgttctgaa gttacgctat cgtgcctagt gccctgaccg gagcagcgca ggttcccctt
      661 gacaagattg gtcatgctct cctaaagttt gctgcgtttt cagacacctt gggtcaaggg
      721 aagagggtgc aaaaccctcg cggtcccgcc gctgtaaatg gggacgaaac ccgtatgtgc
      781 cactgtttgg ttcgccgaac gggaaggcgc ggggagtagg atgaaccatg agccagaaga
      841 cctgcccagg gagagaccct ttattccctc gtgggctggg gaataggcga ggatgagtgt
      901 gcgggtgcaa tcctcagggt tccatgttgg ccctggggat ttttttattt gcgcgccggt
      961 cgaatcaagt ttcttgctcg attcgtcgcg ggatctctcg cgatcctctg ctatgtcgta
     1021 ttgccttgcc ccgcatttgc atttggagac tctacgggag aggacacggt gatgaaacgc
     1081 cgccaacagg gcattctgac cgggatgccg ttcatggcca acattactcc gcgtacgttc
     1141 atcgacgacg cagggcgcaa actctatgtg gccaaggctc cgacgcgggt ggtgtcgctg
     1201 gcgccgagta ttaccgaaat gttgttcgcg cttggattgg acgaacaggt tgtcggcgtc
     1261 acagacttct gcaattttcc ggcagccgcc gccgcgaaac cgaaagtcgg ctacagcaat
     1321 cccaacctgg aatcgctgat cgcgctccgt ccagagctga tcgtggcgcc gctcgagttt
     1381 catcgtgcca atgtgctggc caagctggag gaactgaaaa ttccggtgct cctgctcgaa
     1441 gccacgtcgt tggaacaggt gtttgcgcac attcatacat tggggcggat cttcgatcgc
     1501 tcgaccgccg cccatgcgat gaccgcagag atgcggcaac aaatggcgga gatcagtcgc
     1561 cgaacagaag gcctggcgcg taccagggtg ttgtacgtca tcaatagcca gccgctgatc
     1621 acggtggggc caggcagtta catccatcag atgatcgggt tggcgggagg gaccaatatc
     1681 gctgccgcag cggccacagc ctatccgcgt ctgacgatgg aaacggtgtt gaaggaagat
     1741 cccgaaattc tgatcttccc acgggggtct gtcgagacgg tgccgcgaag cgagcaggag
     1801 gcctggcgac gctggaccac gctgacagcc gtgcagcaga accgcctccg ggaagtgtcg
     1861 gcggatgcgc tcaatcgacc aggcccgcgc gtcatgcagg ggttggaagc actcgccagg
     1921 gtcatccatc ctgaagcgtt cgattctgaa ccgatgacaa tcaagccatg atgccatctg
     1981 ccggtcaccc cacgtcacct cctccagcgc tgacttcgcc atccctctcg cccaatctca
     2041 tgcaatccgg tcccagcttt accgcgactg atcggccgtt tcagggcgcg atcctgactc
     2101 gttcgcgctg gtgtgccgtg atgggggcat tgctgctgct ctcggtgatt cttgccatgc
     2161 tgtgtttaca gctgggaacc cagtacatcg gcatggggca cattctcact ctcgtatggt
     2221 ctgccgtagt cgggacctcg aacgaaccgg atgcgctccg gacgacgggg gtgattctcc
     2281 tgcaggtcag gttgcctcgc gtgttgctgg gatttttggt cggaagttgc ctggcggctg
     2341 tcggagtgtc gctgcaggcg ctgctcagga atcccttggc tgatccctat gtgctgggag
     2401 tgtcgagcgg cgcggcgctg ggcgtcgcca tcgcggtcct gttcggaatc ggcaccacag
     2461 tcctcgcgtt gtcgctctta cctgtctgcg gatttgcggg cagcctggtg gcgctgttgg
     2521 tcatctatcg catggcggcg tcctatgagc gactcccgat ccattcggtg ctgctggcag
     2581 gcgtcatcct caacgccatc ttttccgccc tcattatgtt catcacctcc atcatggagc
     2641 cgaaccgctc atttgggatg atggcgtggc tcatgggatc gctcacggcg ccggcctatc
     2701 cggtcctgat cgcactctcc gtctatttgc tcgtcggcct cgtcctgctg gggaagcacg
     2761 tccgggtgct c
//
LOCUS       DAOQEW010000345         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19168_length_2754_cov_5.515006, whole genome shotgun sequence.
ACCESSION   DAOQEW010000345 DAOQEW010000000
VERSION     DAOQEW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19168_length_2754_cov_5.515006"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLS35_19765"
     CDS             <1..190
                     /locus_tag="PLS35_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNI20965.1"
                     /translation="QRRDPIAQCRGLLKVHGLSSEVFDRLEAEIRREIDEAVSFARLS
                     PAPDESELLQHVYARGGV"
     gene            195..1184
                     /locus_tag="PLS35_19770"
     CDS             195..1184
                     /locus_tag="PLS35_19770"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNI20966.1"
                     /translation="MRILTYAQAIREAHAQLLAEDPRVFLIGQGVRNPWYAGTSLQDL
                     DKEFGTHRVIDSPVSENATTGAAIGAAITGMRPIVFHPRMDFMLLAMDPIMNQAANWS
                     YLFAGRVSVPIVIRAVINRGGEQGAQHAQALQALFAHVPGLKVVMPATPYDAKGLLIA
                     AVRDGNPVLYIDDRWLYDLQGDVPEGLYEVPIGSAALRRSGRDVTVLATSYMSKEATK
                     AVETLTQRGIDVELIDVRSIKPWDRNLVYSSVGKTGRLVIADAAWMSGGIAAEIAASV
                     AGEVFHALKAPIGRVCLPDAPAPTSAALEEAYYIGAEEIVSTVEKVLVSSTRS"
     gene            1187..2299
                     /locus_tag="PLS35_19775"
     CDS             1187..2299
                     /locus_tag="PLS35_19775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNI20967.1"
                     /translation="MAYHVPFIDPRSHYAKLKPEIDRAIIDCLANGDLVNRHQLKDFE
                     QHLAQFVGVKYAVGVNSGYHALLFALLGAGVGAGHEVITVAHTFVATVSAIVHCGAQP
                     VLIDVGPDFNMDVDLIERAITPKTKALLPVHLNGRVCDMERILALADKHGLAVIEDAA
                     QALGATFDGKCAGSQGRAGCFSFYPFKVLGGFGDGGAITTNDASLARMATLLRYNGED
                     RATGEYHYHGQTALLDNVQAAVLDVKLRHLPEWIAHRRQIAAAYHRGLAGISQLRIPQ
                     FDESRRQDIFQNYVIRTTVREPLRAYLKEQGVETLVHWQKPMWEHKGLGLLVPDVPET
                     LAICREVVSLPMSAETTEAQVEQIVAALRAFFSTQP"
     gene            2296..>2754
                     /locus_tag="PLS35_19780"
     CDS             2296..>2754
                     /locus_tag="PLS35_19780"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI20968.1"
                     /translation="MILSDYTPVTEKAGDWVTPEALSMVYTRYRFARDFCRGRRVLEV
                     ACGPGVGLGYFGRYADQIIGGDLTSRLLRQARGRLPGTTPLVQLQAEALPLRSASRDL
                     IVCYEALYFFQDAGKFLAECRRVLAPQGQLLLCSVNPEWPEFNPNDHGRRY"
BASE COUNT          528 a          736 c          888 g          602 t
ORIGIN      
        1 gcagcgccgc gatccgatcg cgcagtgccg tggcttgctg aaggtacatg gtttgtcgtc
       61 agaagtcttt gatcggctgg aggccgagat cagaagggaa attgacgagg ccgtgagctt
      121 tgcacggcta tcgccggccc cggatgaaag cgaattacta cagcatgttt acgcgcgggg
      181 gggagtctag gcgtatgcgg atactcacgt atgcgcaggc cattcgagag gctcacgccc
      241 aactcctcgc agaggaccca cgggtgttct tgattggaca gggcgttcgc aatccctggt
      301 atgcgggaac cagcctgcag gatctggaca aggaattcgg gacccatcgc gtgattgatt
      361 cgcccgtgtc ggagaatgcc acgaccgggg cggcgatcgg cgctgctatc accgggatgc
      421 gcccgattgt gtttcatccg cgcatggatt ttatgttgct ggcgatggac cccatcatga
      481 accaggcggc taattggtcg tatctcttcg ccggccgggt gtccgtgccg atcgtgatac
      541 gagcagtgat taatcgaggg ggcgaacagg gcgcgcaaca tgctcaggct ctgcaggccc
      601 tctttgcgca tgtgcccggg ctgaaggtcg tcatgccggc cactccctat gatgcgaagg
      661 ggctgctcat cgcggccgtc cgggacggca atcccgtgct ctacatcgac gatcgatggc
      721 tctacgatct gcagggggat gtgcctgaag gtctgtatga ggtgcccata ggctccgcgg
      781 cgcttcgacg gagtggaagg gatgtgacgg tgctcgccac gtcatacatg tcgaaagaag
      841 ccaccaaggc cgttgagacg ttgacccagc ggggcattga tgtggaattg atcgatgtgc
      901 gttcgattaa accctgggat cgcaatctgg tgtattcgtc ggtcggcaag accggccgct
      961 tggtcatcgc agatgccgcg tggatgagtg gcggcatcgc ggcggaaatt gccgcctcag
     1021 tggcagggga agtctttcat gctttgaaag cgcccatcgg ccgcgtctgt ttgccggatg
     1081 cgcctgcgcc gacgtctgcc gcgcttgaag aagcgtacta cattggcgcg gaagagattg
     1141 tgtccactgt ggaaaaagtg cttgtgtcga gtaccagatc gtagttatgg cctatcacgt
     1201 tccctttatc gacccgcgca gtcactacgc gaaactcaag cccgagatcg atcgggcgat
     1261 catcgattgc ctggcaaacg gcgatctcgt caaccggcat caactgaaag atttcgagca
     1321 gcacctcgct cagtttgtcg gggtgaagta cgccgtcggg gtgaacagcg ggtaccatgc
     1381 cctgctgttt gcattgcttg gcgccggagt cggggccggt catgaagtca ttacggtagc
     1441 gcatacattc gtcgcaacgg tgtcggcgat cgtgcattgc ggggcgcagc cggtgttgat
     1501 cgatgtcggt cctgacttta atatggacgt tgatttgatt gagcgggcca tcacgccaaa
     1561 gacaaaagcg ctgcttcctg tgcatctgaa cggacgggtc tgtgacatgg agcgtatctt
     1621 agcgctggcc gacaaacatg gattggccgt gatcgaggat gcggcacagg cgctcggggc
     1681 gacgttcgac ggcaagtgtg ccggaagcca ggggcgcgcg ggctgtttta gtttttatcc
     1741 cttcaaggtc ctcggtggat tcggagacgg gggggccatt accacgaacg atgcgtcact
     1801 cgcccgcatg gcgacactcc ttcgttataa cggggaggac cgcgcgaccg gcgaatatca
     1861 ctatcatggg cagacggcct tgttggataa cgttcaggct gccgtgctgg atgtgaagtt
     1921 gcgtcatctg cccgagtgga tcgcacatcg gcggcaaatt gccgctgcct atcatcgcgg
     1981 gctggcggga attagccaac tgcgcattcc tcaattcgac gagtcgcggc gccaggacat
     2041 cttccagaac tatgtgatcc gcacgaccgt tcgcgagcca ttgcgcgcct atctgaagga
     2101 acaaggggtc gagacattgg tccattggca gaaacccatg tgggaacaca aaggattggg
     2161 actgctggtt ccggatgttc ccgaaacgct tgcgatctgc cgggaagttg tgtcgttgcc
     2221 gatgagtgct gaaaccacag aggcgcaagt cgaacagatt gtcgctgcac tccgggcgtt
     2281 tttctccacg cagccgtgat cctctccgat tacacccccg tgacagaaaa agccggcgat
     2341 tgggtaaccc ccgaagcgtt gtcgatggtc tatacgcgct atcgcttcgc aagggacttc
     2401 tgtcgaggcc gacgggtgct ggaagtcgcc tgcggccccg gagtggggct tggttatttc
     2461 gggcggtatg cggatcagat tataggcgga gacctgacga gccggttgct tcggcaggct
     2521 cgagggcgcc tgcctggcac tacgccgttg gtgcagctcc aggcggaagc attgccgctg
     2581 cgatcggcat cccgcgacct catcgtttgt tatgaggcgc tctatttttt tcaagacgcc
     2641 ggaaaatttt tggcggagtg ccggcgggtg cttgctcctc aggggcagtt gctgctctgt
     2701 tcggtcaatc cggaatggcc agagtttaat ccgaatgatc atggtcgccg gtac
//
LOCUS       DAOQEW010000346         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19182_length_2752_cov_8.527994, whole genome shotgun sequence.
ACCESSION   DAOQEW010000346 DAOQEW010000000
VERSION     DAOQEW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19182_length_2752_cov_8.527994"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..512
                     /locus_tag="PLS35_19785"
     CDS             231..512
                     /locus_tag="PLS35_19785"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HNI20969.1"
                     /translation="MADVQCVTCGQAGEAITDMLFLGKLEAEIKAKVCKPCWKKWEGM
                     RVMVINEYQVNLGDESGRELVKKQMKAFLKLGEQTDTSKLDQNYRPAGT"
     gene            731..1186
                     /locus_tag="PLS35_19790"
     CDS             731..1186
                     /locus_tag="PLS35_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNI20970.1"
                     /translation="MITQMRVKGLIFDPYNNAYIVVLRDEENSDMLPIWVGKSEASAI
                     GLALESVTAPRPMTHDLMKAFLETFDAKIISVVITDLNDNTYFATIHLMYEDSEYTVD
                     SRPSDAIALALRTSAPIFASEKVIKKQSSEELEQWLENLKPEDFGKLDS"
     gene            1186..1677
                     /locus_tag="PLS35_19795"
     CDS             1186..1677
                     /locus_tag="PLS35_19795"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNI20971.1"
                     /translation="MMDAAAPQHDASELVALSVKQVLDDGNTDTRIVVLKSEDASVTL
                     PIWVGSAEGNAIRLAMEHVVTPRPMSHDLIRSFADHLGVRIERIVITDVKSSTYYASI
                     AFASKGLHRTLDARPSDAIALALRAGCPIYATQDVLNRRTAVHLDAWINKLDANNTET
                     QQA"
     gene            1728..2159
                     /gene="rplM"
                     /locus_tag="PLS35_19800"
     CDS             1728..2159
                     /gene="rplM"
                     /locus_tag="PLS35_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNI20972.1"
                     /translation="MTKTYLEKPANVQRKWYLVDAEGKTLGRLAAKVATLLRGKHKPT
                     FTPHVDTGDHVVIVNAEKVVLTGNKMENKTYSTHSGFPGGLKTLTAEHVHRKDPTKLL
                     TKAIEGMLPKNPLGNHMAKKLRVYAGATHPHQAQKLESLSL"
     gene            2202..2594
                     /gene="rpsI"
                     /locus_tag="PLS35_19805"
     CDS             2202..2594
                     /gene="rpsI"
                     /locus_tag="PLS35_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNI20973.1"
                     /translation="MAAMTQYATGKRKSAIARAWVTAAAGDIVINEKPLEKAFPRPTL
                     RNVIQFPFELAGVAGKYSVRATVYGGGPAGQAGALRHAISKALVIMSAAFRGPIKKEG
                     LLTRDSRVKERKKYGQKGARKRFQYSKR"
BASE COUNT          690 a          760 c          751 g          551 t
ORIGIN      
        1 cttgcgagaa ccggattttg agtccggcgc gtctgccagt tccgccatcc gggcatctgt
       61 ctcgtcctgc gacattcagc tcatgatcct cagatttgag gggacgcatt tttaacacga
      121 acccacaggc tcgtcaatcg ccgcggcgca ctcttttttc tcaacgagcc ggatgctaga
      181 ataccgcgtt tagcatccaa tcagaattcg cgaattgtat ggaggcaacc atggcagatg
      241 tgcagtgtgt cacctgcggc caggcaggcg aagcaattac ggatatgctg ttcctgggca
      301 aacttgaagc ggaaatcaag gcgaaggtct gcaaaccctg ctggaagaag tgggaaggca
      361 tgcgcgtgat ggtcatcaac gagtatcaag tcaatctcgg cgatgaaagc ggccgcgaac
      421 tggtcaaaaa acagatgaag gcgttcctga agctgggcga acaaaccgac acctccaaac
      481 tggatcagaa ttatcggccc gccggcacct agacctcagc ggagaccgcc cgtcacctgc
      541 ctgatcaagc aggtgttcgg gccatccgtt gcccgcatcg ctgcttcaac taatagggat
      601 gtctacgtcg cctacacctc ccctcgccac cccgtcacga caccgaattc attgacactc
      661 tgttttcaga agtgatagag tgccctactt cttaaggttt ttggctgaca tcgggaagga
      721 tactcccgcc gtgattacac aaatgcgggt caaggggctg attttcgacc cctataacaa
      781 cgcctatata gtggtgctgc gagacgaaga gaattcagac atgctgccga tctgggtggg
      841 aaaatcggaa gcgagcgcca tcggcctcgc gctggaaagc gtgactgcgc cgcgtcccat
      901 gacccacgat ttgatgaaag cattcttaga aacgtttgat gccaaaatca tcagcgtggt
      961 gattacagat ctgaacgaca acacctattt cgccaccatt catttgatgt acgaagattc
     1021 tgaatacact gtcgactctc gccccagcga tgcgatcgcc ctcgccctga gaaccagcgc
     1081 accgatcttt gcgagcgaga aggtaattaa aaaacagtcg tctgaagagc ttgaacagtg
     1141 gcttgaaaac ctgaagcccg aagacttcgg caaactggat agctaatgat ggatgccgca
     1201 gcaccacagc acgatgcgtc ggagttggtc gcactgtccg tcaaacaagt gttggacgat
     1261 gggaacaccg atacccgcat cgtcgtcctt aaaagcgagg atgcgagtgt gaccctcccg
     1321 atctgggtgg gctctgcaga aggcaatgcc attcgcctcg ccatggagca tgtggtgacg
     1381 cccagaccga tgagccacga cctcatccgg agttttgcgg atcacctcgg cgttcgaatc
     1441 gagcggatcg tcattaccga tgtcaaaagc agtacgtact acgcgagcat cgcgtttgcg
     1501 tccaaggggc ttcatcgaac attggatgcc agaccaagcg atgccatcgc cctcgcgctt
     1561 cgcgccgggt gcccgattta tgcgacacag gatgtcctga accggcggac ggccgtgcat
     1621 ttggacgcat ggatcaataa actggatgcc aacaatactg aaactcaaca ggcgtgaggc
     1681 ctgatccctc acattagaat cctttcgtgc aggagtagat ggagaccatg acaaaaacgt
     1741 atctcgagaa acccgctaac gtgcaacgaa aatggtacct cgtggacgcc gagggcaaga
     1801 cccttggccg ccttgcggcg aaagtcgcca ccttgctgcg agggaaacat aagccgacgt
     1861 ttacccccca tgtcgatacc ggagaccatg tcgtcattgt gaatgcggag aaggtcgtac
     1921 tcaccggcaa caaaatggag aataagacct attcgactca cagcgggttt cctggcggct
     1981 tgaagaccct cactgccgag catgtccaca gaaaagaccc taccaagctg ttgacgaaag
     2041 ccatcgaggg catgttgccg aaaaatccac ttggtaacca catggcgaaa aagttgcgcg
     2101 tgtatgccgg cgcgacccat ccgcaccaag cacaaaaact ggaatcactc tcgttgtgat
     2161 ttttattctt tgtattcgat cagtgaagga gaccgcctga tatggccgca atgacgcagt
     2221 acgcaaccgg aaaaagaaaa agcgcgatcg cgcgcgcatg ggtcaccgca gccgctggtg
     2281 acattgtcat caacgagaag ccgctcgaaa aagcgtttcc tcgccccacg ctgcggaatg
     2341 tgattcaatt tccgttcgag ttagccggag tggcaggtaa atattccgtc cgcgccaccg
     2401 tctacggtgg tggccccgca ggccaggccg gagctctccg ccacgccatc tcgaaagcgc
     2461 tggtcatcat gagcgcggcg ttccgtggtc ccatcaagaa ggaaggcctt ctgactcgcg
     2521 attctcgcgt gaaagaacgt aagaagtacg gccagaaggg cgccagaaag cgcttccaat
     2581 actcgaagcg gtaacgaacg actggagcga cagggttcaa aagggaaggc tccgcagcct
     2641 tccctttttt attggctcgc tgcaggtggg ccacaatttg tcacgggccg ggaggcgaag
     2701 atgacgtcgg tacgagtagc ggtagcaggg gccagcggat ataccggagc ag
//
LOCUS       DAOQEW010000347         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19213_length_2749_cov_9.574239, whole genome shotgun sequence.
ACCESSION   DAOQEW010000347 DAOQEW010000000
VERSION     DAOQEW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19213_length_2749_cov_9.574239"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            535..810
                     /locus_tag="PLS35_19810"
     CDS             535..810
                     /locus_tag="PLS35_19810"
                     /inference="COORDINATES: protein motif:HMM:NF022960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopK family periplasmic copper-binding protein"
                     /protein_id="HNI20974.1"
                     /translation="MRKLLIPLLLAVSLSALAAGERVTAQTIELKDGGKVFVKKDGTM
                     VHIDAAGNRVKMRNGVTMEAKDGAKYVMKNDALWKTITEKGTLNPKF"
     gene            complement(854..1417)
                     /locus_tag="PLS35_19815"
     CDS             complement(854..1417)
                     /locus_tag="PLS35_19815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNI20975.1"
                     /translation="MLPEEGYGYLPITEVEPATLGAFTCGKPRLDEFLTTQARALHEA
                     RLGFTNVVFHQQFEGPVGYFTLANDAIKLNDSEKFELGFGEHIELAAFPAVKIGRLAV
                     HQDLQRRGVGGALMALLLGDILNTAGLSAARLIVVDADNDDAVLCFYEKLGFETSLWA
                     EDMAKNHTKKRASAATIKMHRDVFKGL"
     gene            complement(1407..1589)
                     /locus_tag="PLS35_19820"
     CDS             complement(1407..1589)
                     /locus_tag="PLS35_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20976.1"
                     /translation="MKKHASSLKAKKRLVVSVESVRPTQAVQERAVHLAAAARRAKEA
                     HFLDKYRIALPPADAA"
     gene            complement(1752..2123)
                     /locus_tag="PLS35_19825"
     CDS             complement(1752..2123)
                     /locus_tag="PLS35_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20977.1"
                     /translation="MICSDAFSSSNDLLLEATSRRLKALGLQTLQATDSGDAETVTAL
                     FAQFIDCAYRSYALEERWLKACASPDREAHLREHTYLIELTAELYMNLMDDDRRTCAS
                     IRQVLQEAFLPHITVRDRGSY"
     gene            complement(2145..>2749)
                     /locus_tag="PLS35_19830"
     CDS             complement(2145..>2749)
                     /locus_tag="PLS35_19830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI20978.1"
                     /translation="SGEYREVLESNAEEYERLSRMISDMLFLAKSENGLIVPGQETFD
                     LADEVMALFDFYGALAEENGVALKLEGSGWVTGDRLMLRRAIGNLLSNALRYTPSGGH
                     ITVTVSVICDNTDTETVHLAVENTGEPIPTEHLSRLFDRFYRVDASRRHSGDGAGLGL
                     AITRSILRAHQGDIEGRTGEDSNIFEMWIPTSIVTPHSAD"
BASE COUNT          558 a          767 c          848 g          576 t
ORIGIN      
        1 agagcggggg cggggctatt taaggccttg aacacaaaat ttttgacact cccgggattt
       61 gcacggtgga gaagttacgc aggcagactt agctgctact gagctgagcc gtcgccgtta
      121 tcgtcggaga ttgctggcaa tggcttagcg tgctttattt tccgttttct gaaacgaccc
      181 gattggcttc gtttgtttcg ctcttgcact ggccttaggc accgcggtcc ccagtaccga
      241 ggctcgacat atgctggatg tcggccaagg ccctgagctt ggctcaggcc tcctcttgct
      301 ggctccgaac gctggtcatc cccgctgcct ctgcgagatg gcgatggctg ccggcctgtt
      361 ccaaggtttg gcgacatgat caccgtgccg ctccgatcac tgttcgtgat ctcggccgac
      421 ctgcggcggc ggtgtcacct caaccctgaa tcggaaatgg aggtgattca gatgtggtag
      481 atcgacccag aaaagcaaat gcaatcaatc taatccatgc gaaggagaat gatcatgcgc
      541 aagctgttga tacccttgtt actggctgtc tcactgtccg ctttggccgc cggcgagcgg
      601 gtcacggcac agaccattga gctcaaggat ggtggcaaag tctttgtgaa aaaggacggg
      661 acgatggttc atatcgacgc cgccggcaat cgcgtcaaga tgcggaacgg tgtgacgatg
      721 gaagccaagg acggagccaa gtacgtgatg aagaacgatg ccctgtggaa gacaatcacc
      781 gaaaagggaa cgctgaaccc gaagttctga ggtggtggcg gatggtcttc ggaccattcg
      841 cttcaactcg cgttcagagg cccttgaaga cgtcccggtg catcttgatc gtggcggcgg
      901 aagcgcgctt cttagtgtgg ttcttggcca tgtcttccgc ccacaggctg gtctcgaaac
      961 cgagcttttc gtagaagcag agaactgcgt cgtcgttgtc cgcatcgacg acgatcaatc
     1021 gcgcagcaga caagcccgct gtattgagaa tgtcaccgag gaggagtgcc atcaaggcgc
     1081 cgcccactcc cctgcgttgc agatcctgat ggaccgcaag tcgcccgatc ttgacggcgg
     1141 gaaaggcagc aagttcgatg tgctcaccga accccaattc gaacttctcg gagtcgttga
     1201 gcttgatcgc gtcgttggcg agcgtgaagt agccgaccgg accttcgaac tgctgatgga
     1261 agaccacgtt ggtgaagccc agtctggctt catgcaaggc ccgcgcctgt gtggtcagga
     1321 actcgtccag ccgaggcttt ccgcaggtga atgccccaag ggtcgcgggc tccacctccg
     1381 tgatcgggag gtagccatag ccctcttcag gcagcatcag ccgggggcag cgcgatccgg
     1441 tacttgtcga ggaagtgggc ttctttggcg cgccgagccg cagcggccag atgtaccgcg
     1501 cgctcctgaa cagcctgagt cggccggaca gactccacgg acacgacaag ccgcttcttc
     1561 gccttgaggg acgaagcgtg tttcttcata agaaggctgc gggcagagtg tttcatggta
     1621 agggcacagt acgtaagaac gccttccacg cggcgggaag gcactcggat actagatcaa
     1681 aaaagagaat gtatccactg gatatatccg gcagatagcg cactccgctc atgaagccac
     1741 agtgtggtgg ttcagtatga gccgcggtcc cggacagtaa tgtgcggcag gaaggcctcc
     1801 tgcagtacct gacgtattga agcgcaggtc cgtcggtcat cgtccatgag gttcatgtag
     1861 agctcggcgg tcagttcgat caggtaggtg tgttcccgaa gatgggcttc ccgatcaggg
     1921 ctcgcacagg ccttgagcca gcgctcctct aacgcgtagc tgcgatacgc gcagtcgatg
     1981 aactgggcaa acaaggccgt gaccgtctcg gcgtctcccg aatccgtcgc ctggagcgtt
     2041 tgcagtccga gggccttcag gcgacgactc gtcgcttcaa gcaaaaggtc gtttgatgaa
     2101 gagaatgcat ctgagcagat catggccgtg agcctgcaaa cggatcagtc cgcgctgtgg
     2161 ggcgtgacga tcgatgtcgg tatccacatc tcgaaaatgt tgctgtcttc ccccgtccga
     2221 ccctcgatgt cgccttggtg cgcgcggagg atggaacggg tgatcgcaag gcccagtccg
     2281 gcgccatcgc ccgaatggcg gcgcgatgcg tccacacggt agaagcgatc aaagagacgg
     2341 gacaggtgtt ctgtcgggat aggctccccg gtgttctcga cggcgaggtg gacggtttcg
     2401 gtgtcggtgt tgtcgcatat gacgctgacc gttacggtga tgtgcccacc cgatggcgtg
     2461 tagcgaagcg cgttggatag aaggttgccg atggcgcgtc ggagcatcag ccggtctccg
     2521 gtcacccatc ctgaaccttc tagcttgagg gccacgccgt tctcttcggc cagggccccg
     2581 taaaaatcga acagggccat gacctcgtcc gccagatcaa aggtttcctg acctggcacg
     2641 atgagaccat tttccgactt ggccaggaac agcatgtccg agatcatgcg cgacaggcgt
     2701 tcgtactcct cggcgttgga ttccagcact tcccggtact cgcccgacg
//
LOCUS       DAOQEW010000348         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19337_length_2738_cov_6.783079, whole genome shotgun sequence.
ACCESSION   DAOQEW010000348 DAOQEW010000000
VERSION     DAOQEW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19337_length_2738_cov_6.783079"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..471
                     /locus_tag="PLS35_19835"
     CDS             76..471
                     /locus_tag="PLS35_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20979.1"
                     /translation="MREAQVRSGMIVGWFLLAAMTGTGSAEQGFDEKYQRDFNIFNPI
                     NQYQPGNPLNPINAYDPANPFNPINRFDPGNPANPINQFNPNNPFNPVNRYRPENPLN
                     PINEFNPTVPFGPLGEWDRNDEGWWKGEG"
     gene            634..951
                     /locus_tag="PLS35_19840"
     CDS             634..951
                     /locus_tag="PLS35_19840"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNI20980.1"
                     /translation="MTQASLLTHVTQTIIDRFHPKRIMVFGSHARGEAGPDSDLDLFI
                     EMETPRRPPDRAIEVSQAFGLRAWPMDIVVYTPEEVRRLRHVKGTLLSVIEKEGKVLY
                     ERG"
     gene            938..1336
                     /locus_tag="PLS35_19845"
     CDS             938..1336
                     /locus_tag="PLS35_19845"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNI20981.1"
                     /translation="MNGAESNFDAWLRKAQHDLLNIENNLAAKDIPWDTVCFHAQQVA
                     EKVLKAFLVHRGRDLTKTHDLVALLAQCVACDEGLAVLEDDCRKLTSYGVAARYPDDL
                     FEPEETDGRDVVAAAHRVRTKILLLLPGNR"
     gene            1606..1986
                     /locus_tag="PLS35_19850"
     CDS             1606..1986
                     /locus_tag="PLS35_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20982.1"
                     /translation="MREAQVRSGMIVGLFLLVAMTGIGRAEPGFDEKYQRDFNIFNPI
                     NQYQPGNPLNPINAYDPANPFNPINRFDPGNPANPINQFNPNNPFNPVNRYRPENPLN
                     PINEFNPTVPFAPLDAGGVMGKRR"
     gene            2160..>2738
                     /locus_tag="PLS35_19855"
     CDS             2160..>2738
                     /locus_tag="PLS35_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HNI20983.1"
                     /translation="MFEQAFRNIDDVLRKEAGCTTELDYTEQTSWLLFLKYLDGLEED
                     RADEARLEGKRYSFILDKDYRWDNWAAPKSKDGKLDHNKAMTGDDLRDFVTLKLFPYL
                     HSFKQKASGPNTIEYKIGEIFGEIKNKIQSGYNLREIIDHIDELRFRSQKEKHELSHL
                     YEAKIRNMGNAGRNGGEYYTPRPLIRAMVQVVK"
BASE COUNT          651 a          761 c          790 g          536 t
ORIGIN      
        1 tgagcgacgc gagaacgccg ttggcggact ttttccgcat cctgctagaa gtgaataggc
       61 acaagggagg cggctatgcg agaggctcag gtgcgaagcg gaatgatcgt cggatggttt
      121 ctgctggcgg cgatgaccgg gacaggcagc gctgagcagg ggttcgatga gaagtaccag
      181 cgcgacttca acatcttcaa ccccatcaac cagtaccagc cgggcaaccc gctcaatccc
      241 atcaacgcct acgatccggc gaacccgttt aatccgatca accgcttcga ccccggcaac
      301 cccgccaatc cgatcaacca gttcaacccc aacaatccct tcaatccggt gaatcgctat
      361 cggccggaga atccgttgaa cccgatcaat gagttcaatc ccaccgtgcc gtttgggccg
      421 ttgggggagt gggaccgaaa cgatgagggg tggtggaaag gagaaggtta ggcggatggc
      481 agtggccgag tgggaggatg gagaacgaga gcttttccat gaaagatgag agcgccgcta
      541 cagatggtgc tttcttaaaa ggaaacgggt attcttcggc gacatggccg gtggaccgtc
      601 tagcctacca aatgcctcac ggagactgca tgtatgacac aggcttcatt actgacccat
      661 gtgactcaga ccatcatcga tcgctttcac ccgaagcgta tcatggtctt tggtagccac
      721 gcccgagggg aggccggccc agatagcgac ctggacctct ttattgagat ggagacgccc
      781 cgccggcctc ctgatcgggc gatcgaagtg agccaggcgt tcgggctacg tgcatggccg
      841 atggatatcg tggtgtatac gccggaagaa gtgcggcgct tacgccatgt gaagggcaca
      901 ttgctgtcag tcattgagaa ggagggcaag gttctgtatg aacggggctg agtccaattt
      961 cgacgcgtgg ttgcgcaaag cccaacatga tctgctgaac atcgaaaaca accttgcggc
     1021 caaggacatt ccctgggaca cggtctgctt tcacgcgcag caagtggctg agaaagtatt
     1081 gaaggcgttt cttgtccatc gtgggcgcga tcttacgaag actcacgact tggttgcctt
     1141 gcttgcccag tgcgtggcgt gcgatgaagg gctcgcggtg ttggaggatg attgccggaa
     1201 actgacatct tacggtgttg cggcacggta tccggatgac ctgttcgagc ctgaagagac
     1261 ggacggtcgt gatgtggtag ccgccgccca ccgcgtgcgc acaaaaattc tcctactcct
     1321 tccggggaac cggtgaatga ctgcaaactc ccggggtcgg tcgagattct ccctccgcat
     1381 aaccgaagtg ttattggtct atcgcgatta gtcctcagtc cgtttctgtg ctccctctcc
     1441 agactcatca cgcaaccctc aacatgtctg acatccctcc gcttccggtc cgaacggcgc
     1501 ttgttacctg ttacgtctaa cgtttcccat cccgttgtga ccccggatgt cacggcaggg
     1561 gagtatgctg ccggcaaggt gcacagaggc ataagggagg ccatcatgcg agaggctcag
     1621 gtgcgaagcg gaatgatcgt cggattgttt ctactggtgg cgatgacagg gataggccgg
     1681 gcggagccgg gcttcgacga gaagtaccag cgcgacttca atatcttcaa ccccatcaac
     1741 caataccagc ccggcaaccc gctcaatccc atcaacgcct acgatccggc gaacccgttt
     1801 aatccgatca accgcttcga ccccggcaac cccgccaatc cgatcaacca gttcaacccc
     1861 aacaatccct tcaatccggt gaatcgctat cggccggaga atccgttgaa cccgatcaat
     1921 gagttcaatc ccaccgtgcc gtttgcgccg ttggatgctg ggggtgtgat ggggaagagg
     1981 cggtgagcgg ttcgcccgtc atctgagata gttccacgta cgaaacgcgg tcaactgcgg
     2041 agatagccct gtaggccgga gagccgacct ggaccgtaaa aatgccggtg ataaaaggtt
     2101 cgggaaagag tatcctgcgg cgcgaatcgt acgaattgta tcaggtggag agcggctgca
     2161 tgtttgaaca agcctttaga aatatcgacg acgtactcag aaaagaagcc ggttgtacga
     2221 ccgagttgga ctataccgag cagacctcgt ggttgttgtt cctcaagtac ctggatgggc
     2281 tggaggagga cagagcagac gaagctaggc tggagggcaa gcgttacagc ttcatcctcg
     2341 acaaagacta ccgctgggac aactgggctg caccgaaaag caaagacggc aagcttgatc
     2401 acaacaaggc gatgacaggc gatgatctgc gcgacttcgt cactctcaag ctctttccgt
     2461 acctacacag cttcaagcag aaggcgagcg ggccgaacac tatcgaatac aaaattggcg
     2521 aaatcttcgg cgagatcaaa aacaagatcc agagcggcta caacctgcgc gagatcatcg
     2581 accacatcga cgagctgcgt ttccgctcgc agaaagaaaa gcacgaactc tcgcatctct
     2641 acgaagccaa gatccggaac atgggcaacg ccgggcgcaa cggcggcgag tactacaccc
     2701 cgcgccccct catccgcgcc atggtgcagg tcgtcaag
//
LOCUS       DAOQEW010000349         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19459_length_2727_cov_6.497006, whole genome shotgun sequence.
ACCESSION   DAOQEW010000349 DAOQEW010000000
VERSION     DAOQEW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19459_length_2727_cov_6.497006"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLS35_19860"
     CDS             complement(<1..610)
                     /locus_tag="PLS35_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNI20984.1"
                     /translation="MQYKSQAVAKPYFIAALALFVAQILFGLIMGLQYVWGDFLFPHI
                     PFNVARMVHTNALIVWLLMGFMGAAYYLVPEEAETELHSPKLAIALFWIFLVAAGLTV
                     VGYLTVPYATLAKMTGNDLLPTMGREFLEQPLLTKAGIVVVALAFLFNITMTILKGRK
                     TAINTVLLLGLWGLAVMFLFSFYNPDNLVKDKFYWWWVVHLWV"
     gene            complement(637..1071)
                     /locus_tag="PLS35_19865"
     CDS             complement(637..1071)
                     /locus_tag="PLS35_19865"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20985.1"
                     /translation="MSGTFTTSTARNIFYGGTLFFLLVFLALTFQNEQIIPKRDRREM
                     LANPQIAVGKKLWETNNCIGCHTLMGEGAYFAPELGNVVVRLGSKEAVKAFIKGRPAE
                     GIPGRRSMPQFNFSDKDLDAIADFLEYVSKIDAAKWPPNVQG"
     gene            1564..2220
                     /locus_tag="PLS35_19870"
     CDS             1564..2220
                     /locus_tag="PLS35_19870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI20986.1"
                     /translation="MNDKGILIVSADAANARILTRMLRRRRFAVTSTRDPAGALEAAG
                     ATRPGYVVLEQDLPERSGLSLIRPMLATVPDSKILVLASCATIESAVDAIKLGAIDYL
                     AKPAGLDEILRGLGLDGVADAAESGPGGETAATGLDQIQWTQIVGALARHEGNVSATA
                     AALRMNRRTLQRKLEAHQSRTGKDVAAGIRASSDRRRRIELRRQWEASRRGVVSPTGT
                     "
     gene            complement(2305..>2727)
                     /locus_tag="PLS35_19875"
     CDS             complement(2305..>2727)
                     /locus_tag="PLS35_19875"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNI20987.1"
                     /translation="GCQTNRPFGIAGESFENPKYLDKMVETMGAEPLTKYEQKVTCCG
                     GALAFSEPDKAQKQIRDIVESAYDHGADMIVTPCQLCQANVEIYQSEINKKQGTKLNL
                     PVVYYSQLMTVAYGGSAKEAGLDGQLIKAAKLEGIASK"
BASE COUNT          494 a          823 c          877 g          533 t
ORIGIN      
        1 cgacccagag gtgcacgacc caccaccagt agaacttgtc cttcaccagg ttgtcggggt
       61 tgtagaagga gaacaggaac atcaccgcca gaccccagag gcccaggagc agcacggtat
      121 tgatcgccgt cttgcgtcct ttcaggatcg tcatcgtgat gttgaacagg aaggccagcg
      181 cgacgaccac gatacccgcc ttggtgagca gcggttgctc caggaactcc cgccccatgg
      241 tcggcagcag gtcgttgccg gtcatcttcg ccagggtggc gtaggggacg gtcaggtacc
      301 cgacgacggt gaggccggcg gcgacgagga atatccagaa cagggcgatg gccagtttcg
      361 ggctgtgcag ttcggtctcg gcttcctcgg gtacaaggta gtaggccgcc cccatgaagc
      421 ccatcaacag ccagacgatc agtgcattgg tgtgcaccat ccgggccacg ttgaagggga
      481 tgtggggaaa caggaagtcg ccccagacat actggaggcc catgatcagg ccgaacagga
      541 tctgggcgac gaagagcgcc agcgccgcga tgaaataggg tttggcgacc gcttgggact
      601 tgtattgcat gtggtttctc ctatcggatc tcgatgtcag ccctggacgt tgggcggcca
      661 cttggcggcg tcgatcttcg acacgtactc gaggaagtcg gcgatggcgt cgaggtcctt
      721 gtccgagaaa ttgaactggg gcatggaacg gcgtccgggg atgccctcgg ccggccgccc
      781 cttgatgaag gccttgaccg cctccttgct gcccaggcgg acgacgacgt tgccgagttc
      841 gggcgcgaag taggcgccct cacccatcag cgtgtggcag ccgatgcagt tgttggtctc
      901 ccacagcttc ttgcccacgg caatctgcgg attggccagc atctcgcggc ggtcccgctt
      961 ggggatgatc tgctcgttct ggaaggtcaa cgccaggaac acgagcagaa agaacagggt
     1021 tcccccgtag aagatgttgc gtgcggtgct ggtggtgaat gtgccgctca tgggcgatgt
     1081 cctcccggtt caatgcgcag aagacctgcg ccgggccgga atctattggc gcgcgcgatg
     1141 gcggtaattg accgccatca atccggagtc ggccggagcg attcagcgcg gcttgtaact
     1201 aattgatttg aaggaatgta ttttggattc agcgctacat tttgtcgcag cgcaagatat
     1261 tgcagtacag actgccaaaa cgggtcgggc aaataccggc ccggccatgg ggtcgtgcat
     1321 ccaatacgga tttccgcgag tgatttgcag caatccggtc gtcagtccac gtctgtcggg
     1381 ggtgccaata acggcagtca cggaacgcca ataatggcgt tcattcaatt ccgtggagtg
     1441 tgctccggcc gccccggtct tcccaagtca ttgattcgtc ggcgcggttt tcccggccgg
     1501 tcgcgatctc cttccggcac gaagtttgta gggaagggag accgcagttc ggattgcttc
     1561 ccgatgaatg acaaaggaat cctgatcgta tcggcggatg cggccaacgc ccgtatcctc
     1621 acccgcatgc tccgtcgacg ccgcttcgcc gtgacctcga cccgcgatcc tgccggtgcg
     1681 ctcgaggcgg ccggcgccac ccgtcccgga tacgtggttc tggagcaaga cctgccggag
     1741 cggtcggggc tctcgttgat tcgtccgatg ttggccacag tgcccgattc gaagatattg
     1801 gtcctggcct cttgcgcgac gatcgaatcc gccgtcgacg ccatcaagct aggcgcgatc
     1861 gactacctgg ccaaaccggc gggactggac gaaatcctgc gcgggttggg cctggatggc
     1921 gtcgccgacg ctgccgagtc cggccccgga ggcgagaccg cggcgacggg cctcgaccaa
     1981 atccaatgga cccagattgt tggcgccctc gcgcggcacg agggcaatgt gtcggctacc
     2041 gccgccgcgt tgcgcatgaa tcggagaacc ctgcagcgaa agctggaagc gcatcaatcg
     2101 cgcacgggga aggacgttgc ggccggaatc cgtgcctcga gcgaccgtcg ccggcgcatc
     2161 gaactgcgtc gccaatggga agcatcgcgg cgcggtgtcg tttccccgac ggggacctga
     2221 tcccggcccg gcgcatcggg ctccgcgcaa agaaagacgg ccgcatgtcg cggccgtctg
     2281 cgcgctttcc ggacccacgg cctcctattt cgaggcgatt ccctccagtt tcgcggcctt
     2341 gatcaactgc ccgtccagtc cggcctcctt ggccgagccg ccataggcca cggtcatgag
     2401 ctgcgagtag tagaccaccg gaagattgag cttcgtgccc tgcttcttgt tgatttccga
     2461 ctggtagatt tccacgttgg cctggcagag ctggcagggc gtgacgatca tgtccgcccc
     2521 gtggtcgtaa gcggattcga cgatgtcacg gatctgcttc tgcgccttgt caggttccga
     2581 gaaggccagc gcgccgccgc aacaggtgac cttctgttcg tatttcgtca gcggctcggc
     2641 tcccatggtc tcgaccatct tgtccaggta cttggggttc tcgaaggact cgcccgcgat
     2701 gccgaagggc cggttggtct ggcagcc
//
LOCUS       DAOQEW010000350         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19473_length_2726_cov_12.776863, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000350 DAOQEW010000000
VERSION     DAOQEW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19473_length_2726_cov_12.776863"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2726)
                     /locus_tag="PLS35_19880"
     CDS             complement(<1..>2726)
                     /locus_tag="PLS35_19880"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNI20988.1"
                     /translation="SSRGGKKRSFHQELVLNRWVLGFFQGGTLAALKMRLGDDRFEGI
                     DEDGQTKFFHELIRGLFDPNKVPEADLRRYDLNVVAHWQAITAQRNKLEGHELQMKYF
                     QYLSLLFTELYLDWYFNHRQDLLDGLNEEMTRYRAETGAEPFRDFEADDLNKIAFWNA
                     TGSGKTLLLHVNIRQYLHYFQAGRSDHYPSKIVLLTPNEGLSRQHLEELHLSGFGFSQ
                     FFNKAQSPARGTIEIIDINKLGDEMGDKTVAVDAFEGNNLVLVDEGHRGTGTAAGAWM
                     ARRDALVRGGFAFEYSATFGQAVAKGMTVAAAEEDIQKKRAKMLFNTTSLRSLDDGQK
                     AQLALTADDKRRARITATREIYAKCILFDYSYKFFYEDGYGKESLILNMNGDAYEQAD
                     NARKYFTACLLAYYQQLWLWSTHRDKLTDFNIEKPLWVFVGNTVSGEESDILEVVNFL
                     ADFLNSEAQIKSWLTDLIADKAQILDAKGNNIFSGRFTPLMGFSGRVDELYADILLRV
                     FNASAKQRLKLVNIKSSKGELALRVGDADPFGLINIGDDAGFFGMAEDVEAFDSERDD
                     FGGALFGTLNNKDSRLNVLIGSRKFTEGWSSWRVSTMGLLNMGQGEGSQIIQLFGRGV
                     RLKGKGFSLKRTLPQDRPKGVHLDKLEALNIFGVRASYMAAFKDYLREEGITPSDEVL
                     ELDFPTRANLPKGKLKTLALKDGYKDNQKLGFKRTHFPWLYEIPAQFQGKIKPPHVVL
                     DLYPRVEALSSKDKGTTPTTEARNKGKLNQTLFPAFNWDRIYLALQDYKLQRSWSNLR
                     LERQKLIDFCAGTQDWYTLFIPAAELNVTTFADIRKQEDILLRLLTDYTDRFYKALKT
                     GYEGQFYDITHIDEDHGSMLKLYQFEIENSDDGLEYQAKLEVLKKLVADGKIG"
BASE COUNT          487 a          835 c          798 g          606 t
ORIGIN      
        1 ccgatcttgc cgtccgccac cagcttcttc agcacttcca gcttcgcctg atactccaga
       61 ccgtcgtcgc tgttctcgat ctcgaactgg tacagcttga gcatcgaccc gtgatcttca
      121 tcgatgtggg tgatgtcgta gaactggcct tcgtagcccg tcttcagggc cttgtagaag
      181 cggtcggtgt agtccgtcag cagtcgcagc aggatgtctt cctgcttgcg aatgtcggcg
      241 aaggtcgtca cgttcagttc cgccgccggg atgaacagcg tgtaccaatc ctgcgtgccc
      301 gcacagaagt cgatcagctt ctgccgttcc aaccgcaggt tgctccagct gcgctgcaac
      361 ttgtaatcct gcaacgccag atagatgcga tcccaattga aagccgggaa cagcgtctga
      421 ttcagcttgc ccttgttgcg ggcttccgtc gtgggggttg tgcccttgtc cttgctggac
      481 agcgcctcga cacgcgggta caggtcgagt accacatggg ggggcttgat cttgccctgg
      541 aactgcgcgg gtatttcgta cagccacgga aagtgcgtgc gcttgaaccc aagtttctgg
      601 ttgtccttgt agccatcctt cagcgccaag gtcttgagtt tgcctttggg caggttggcc
      661 cgcgttggga agtccagctc cagcacttcg tcactgggcg tgatgccttc ctcgcgcagg
      721 tagtccttga acgcagccat gtagctggcg cgcacgccga agatgttcag tgcctccagt
      781 ttgtccagat gcacgccctt gggccggtcc tgcggcaagg tgcgcttgag cgagaagccc
      841 ttgcccttga ggcgcacccc gcgtccgaac aactggatga tctgcgagcc ttctccctgg
      901 cccatgttta gcaggcccat cgtggacacg cgccaactgc tccagccttc cgtgaatttg
      961 cgcgagccga tcagcacgtt gaggcggctg tccttgttgt tcagcgtgcc gaacagcgcg
     1021 ccgccgaagt cgtcgcgttc gctatcgaag gcctctacgt cctccgccat cccgaagaag
     1081 cccgcatcgt cgccgatgtt gataaggcca aagggatcgg catcgcccac gcgcaaggcg
     1141 agctcgcctt tgctactttt gatgttgacc agcttcagcc gttgcttggc ggaggcatta
     1201 aacacgcgta gcaagatgtc ggcgtatagc tcgtccacac gaccgctgaa gcccatcaag
     1261 ggtgtgaagc gcccgctgaa gatattgttg cccttggcgt ccagaatctg cgccttgtcc
     1321 gcaatcaggt cggtcagcca gctcttgatc tgcgcttcgc tgttcaggaa gtcggccagg
     1381 aaattcacca cttccaggat gtccgactcc tcgcccgaca ccgtgttgcc gacaaaaacc
     1441 cagagcggct tctcgatgtt gaagtccgtc aacttgtcgc ggtgcgtgct ccacagccat
     1501 agctgctggt agtaggcgag cagacaggcg gtgaaatact tgcgagcgtt gtcggcctgc
     1561 tcgtaggcat cgccattcat gttgaggatc agcgactcct tgccgtagcc gtcctcatag
     1621 aagaacttgt acgagtagtc gaaaaggatg cacttggcgt aaatctcacg ggtagcggtg
     1681 atacgcgcac ggcgcttgtc atccgccgtc agcgccaatt gcgccttttg cccatcgtcc
     1741 aaactgcgca ggctggtggt gttgaacagc atcttggcgc gctttttctg gatgtcttct
     1801 tccgccgccg ccacggtcat gcccttagcc actgcctgcc cgaaggtggc cgagtactcg
     1861 aaggcaaaac cgcctcgcac cagcgcatcg cgccgcgcca tccacgcgcc cgccgccgtg
     1921 cccgtaccac ggtggccttc gtccaccagc accaggttgt tgccctcgaa ggcatccacg
     1981 gcgacggtct tgtcgcccat ctcatcgccg agcttgttga tgtcgatgat ttcgatggtg
     2041 ccgcgcgcgg gtgactgggc cttgttgaag aactgcgaga acccgaagcc ggacaggtgc
     2101 agctcctcca gatgctgacg gctcaaccct tcgttgggcg tgagcaggac gatcttgctc
     2161 gggtagtgat cgctgcgccc cgcctggaag tagtgcaggt actggcggat gttgacgtgc
     2221 agcagcaggg tcttgccgct gccggtggca ttccagaagg cgatcttgtt caggtcgtcc
     2281 gcttcgaaat cgcggaacgg ttctgcgccc gtctcggcac ggtagcgcgt catctcctcg
     2341 ttcaggccat cgagcaagtc ctgacggtgg ttgaagtacc agtccagata cagctcggtg
     2401 aacagcagag aaaggtactg gaagtacttc atctgcagtt cgtgaccttc cagcttgttg
     2461 cgttgggcag tgatagcctg ccagtgggcg acgacgttca agtcgtagcg ccgcaggtcg
     2521 gcctcgggca ccttgttggg atcgaacaag cctcggatca gttcgtggaa gaacttggtc
     2581 tggccgtcct cgtcgatgcc ctcaaagcgg tcatcgccca agcgcatctt gagcgccgcc
     2641 agcgtgccgc cctggaaaaa acccagcacc cagcggttga gcaccaattc ctgatggaag
     2701 ctgcgcttct tgccgccgcg actgga
//
LOCUS       DAOQEW010000351         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19519_length_2723_cov_9.097451, whole genome shotgun sequence.
ACCESSION   DAOQEW010000351 DAOQEW010000000
VERSION     DAOQEW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19519_length_2723_cov_9.097451"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..2036
                     /locus_tag="PLS35_19885"
     CDS             174..2036
                     /locus_tag="PLS35_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20989.1"
                     /translation="MKTNRVVLVGIALLSLLASVGCESIKSRFSRQALPDLGPPVPLT
                     VHMEIDPSLSKAKTEYIDNCSRIHAFSIGPTVEDTLIQAAHQTFRTVTIPGSQAAGGK
                     PDVTIRVRMLDPRFKLQTDALYDRAPAELSLDALAEFFDASGAPLGERPLQATRKERL
                     QLELTQQRCDYIVDPLLQDTSTMLATQFMQEARVLLAPATPPPAAATTATPTEPSSKA
                     TAVPAAPAATPVQPLSFKATLLDENGNLIIESGERIRVRVDVVNTGTQLVQDSTVKLT
                     GPQTVLTQFPATTLATGTIPAAGSKSLEFVATMPQSPSLQRAELQVALLNASGQAAAP
                     PQTLPLSFQPTAALLEDVDHIPAATSGAQRPGDYLLAIGLSSYREQDIAARKYASLDA
                     ETVAAYFQTLGGLPQNNVRLLRDWSALRPDIEEALLDWLPAKTTKESLVVVYFAGQAM
                     VNAAGDTFLIPYDGSMGSPTRLYPLKELEAALGRLKAKQVLFVFDGTVLKNGADGRTK
                     AAAPKWLGTSGSVLHMIATTGFGKSLESETWHHGLFTYYLLRGLRGEADLNRNGEVTI
                     AEVTAYINRKVPGAARSTFKQEQQPQILPTLRGSEKGLDTVLTKPTMTFPSDQP"
     gene            complement(2232..>2723)
                     /locus_tag="PLS35_19890"
     CDS             complement(2232..>2723)
                     /locus_tag="PLS35_19890"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI20990.1"
                     /translation="LFTRWVQHKGGVLMGYFSKFVGVCAAVTLLSVAVVGAEEKDPLK
                     PRVAPDQMADAKAMKNPVASTPESIAKGKALYEGKGTCFNCHGKEGKGDGPAGAILNP
                     SPRNFTNCKFHKKRKDGELFWVIKNGSPGTGMVSLVPAAITEEEAWTIINYERSFCKG
                     GDE"
BASE COUNT          581 a          870 c          734 g          538 t
ORIGIN      
        1 gaggagcaag gagcaacgcg actcccgcgc cgatcaatgc gccgtttagg cgaggcgcgg
       61 cggtaggata aacgaggccg gtgagggtcg caagccggcc cagcgcttgg ttgcaatgcc
      121 cccctcattc ctgtaccgtt tcccattctt attctgccgt cgaggaggcc tgcttgaaga
      181 caaaccgcgt agtccttgtt ggaattgccc tactctccct gctggcttcc gtcggctgcg
      241 agtccatcaa atcgcgattt tcccgtcaag cgttgccaga tctaggccct cctgtccccc
      301 tcaccgttca catggagatc gatccgtctc tctccaaagc caagacggag tacatcgata
      361 actgcagccg catccacgcc ttctcgatcg ggccaaccgt cgaagacaca ttgatccagg
      421 cggcacatca aacgtttcgc accgtcacga ttccgggaag ccaggcggcc gggggaaaac
      481 ctgacgtgac gatacgcgtg cgtatgctgg atccacgctt caagctgcaa acggacgccc
      541 tctatgatcg tgcccctgcg gaactcagcc tggatgccct ggcggaattt ttcgatgcgt
      601 ccggcgcgcc gctcggtgag cggccgctcc aagccacccg gaaagaacgt ttgcaactcg
      661 aactgacgca acagcgctgc gattacattg tcgatccact gttgcaggat acgtctacca
      721 tgctggccac gcagttcatg caagaggcac gggtgctcct ggccccggcg acaccaccgc
      781 ctgcggctgc cacgactgcc acgcctaccg agccaagctc gaaggcgacc gccgttccag
      841 ccgctcctgc tgcaacgccg gttcagccgc tttcgttcaa agccacgttg ttggatgaga
      901 acggcaatct gatcatcgaa agtggtgaac gaatccgcgt gcgagtcgac gtggtcaata
      961 cgggcacgca gttggtgcag gacagtaccg tcaaactgac aggcccgcag acggtcctta
     1021 cgcagttccc tgccacaacg ctggccaccg ggaccatccc ggcggccggt tccaaatcgc
     1081 tggaatttgt tgcgacgatg cctcaatccc cgtcgcttca acgggcggag ttacaggttg
     1141 cgctgcttaa cgcctcagga caagcagccg ctccaccaca aacattaccg ctctcctttc
     1201 aaccgacagc cgcgctcctc gaggacgtgg atcacattcc tgccgctacg tcaggagcac
     1261 agcgtccagg cgactatctc cttgccatcg gactcagcag ttatcgcgaa caggacatcg
     1321 cggcgcgaaa atatgcctcg ctcgatgcag agacggtcgc ggcctatttt cagaccctcg
     1381 gcggtttacc tcaaaacaac gttcgcctcc tgcgggactg gagcgccctt cgccccgaca
     1441 tcgaagaggc cctgctcgat tggcttcctg caaaaacgac caaagagtcg ctggtggtcg
     1501 tctactttgc ggggcaagcg atggtgaacg ccgccggcga taccttcctg atcccctatg
     1561 acggctcgat gggatcaccc actcgcctgt atccactcaa ggaattggaa gcggcgctgg
     1621 gaagattgaa agccaagcaa gtgctcttcg tctttgacgg gacagtcctc aagaatggag
     1681 cggacgggcg cacgaaagca gcggctccga aatggctcgg gacatcagga tcggtactgc
     1741 acatgattgc gacgaccggc ttcggcaaga gcttggaatc cgaaacgtgg catcacggat
     1801 tgtttacgta ctatctcctg cgaggtcttc ggggcgaagc cgatctcaat cggaatggcg
     1861 aggtcacgat tgcggaggta accgcttaca tcaatcgcaa agtaccgggc gcagcgcgga
     1921 gtaccttcaa acaggaacag caaccgcaga tcctcccgac actgcgtgga tcggagaagg
     1981 gtctcgacac cgtgctcacc aaaccaacga tgacgttccc ctctgaccaa ccgtaaaatg
     2041 cggtgcattt cgccctttat ccgtcacggc agaaagccgg gatgaggtag acggggcatt
     2101 ccttcctgtc acacgttact cgggactcac acgggattgc gccgacattc gtcagcgcga
     2161 tacaaaagaa aaggggcggc gaggataccc tcgccgcccc tccgattcag cctatcggcc
     2221 gagaatcgtg actattcgtc tccacccttg cagaagctgc gctcataatt gatgatcgtc
     2281 caggcttctt cttcggtgat tgccgcaggg accaacgaaa ccatgccggt gcccgggctg
     2341 ccgttcttga tcacccagaa cagttcgccg tctttccgct tcttgtggaa cttgcagttg
     2401 gtgaagttac gcgggctcgg attcaggatc gcgccggcag gaccatcgcc cttgccttcc
     2461 ttgccatggc aattgaagca ggtgcccttg ccctcataca atgccttgcc cttggcaatg
     2521 ctctccggag tcgaagcaac cgggttcttc atcgccttcg cgtccgccat ctgatccggc
     2581 gcaacacggg gcttcaacgg atccttttct tctgcgccca ccaccgccac ggacaacaag
     2641 gtgacggccg cacacactcc gacaaactta gaaaaatacc ccatcagaac tcctcctttg
     2701 tgttgaaccc accgcgtgaa caa
//
LOCUS       DAOQEW010000352         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19923_length_2690_cov_9.572676, whole genome shotgun sequence.
ACCESSION   DAOQEW010000352 DAOQEW010000000
VERSION     DAOQEW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19923_length_2690_cov_9.572676"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1645
                     /locus_tag="PLS35_19895"
     CDS             164..1645
                     /locus_tag="PLS35_19895"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNI20991.1"
                     /translation="MTTSARRVIALAPMPPEKSAYALARYSRSPDSIEDSIKWVHGHS
                     SEKFWEQFYFDYGHGSIADLGHVIICFEQISELAAIRLEDEPVWDGQAKSSRYQNFAT
                     SSWFVPATIRGQEIEASYLGILRGLATVYRALHDPLSQFLTEREPRPESMTPAAYQRA
                     ITARAFDVTRYLLPLAAQTNVGQVVSIRTLEKQITRLLSSQIPELRQIGEDLQEACRK
                     PPVNLWGELSGQSAGLGEPMAPTLARYAKPSVYQAEVYSDLARYAKDVLKGTGLDQPS
                     AYGAAEPVDLIEPHDPLDEVVTTLLYRASHAPYRKILALVQDWTEKQKQDTLEVAFQK
                     RGPYDELIKEFRSGYAFIFDVMMDIGGWRDMHRHRRCQQVQQNFTTVHGFETPPILAE
                     AGLEQEYREAMGHVKSDIERLKKSSQEAALYAIPFGFSVRCLFKMDYAEAEYIAKLRS
                     GVKGHWSYRTIAWLMKEKMTARYPILGARMQATPPDVQDALTR"
     gene            1666..>2690
                     /locus_tag="PLS35_19900"
     CDS             1666..>2690
                     /locus_tag="PLS35_19900"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI20992.1"
                     /translation="MNQPLNQIETALLAGDWDRLGYEAGVSVRALEAADEKDPRPYFA
                     LNVTHLIRGEFAEAWRVHAHALQETADIEKVGEWVKAVLKQYPDNPYLNLVQGMFLAQ
                     SGQSEQSVVFYKEAARLAPQSAYPHYFLAQIHERANHLEMAIKEYREAVKLDPAFAAA
                     RTNLGVAYQEQGRLEMAIPQYREVIKLNPNDGVAHANLGCALAEQGKFEPALQAYKEA
                     LRLNPNDAEIHFALGGVYETKGRMDLAVKEYRAATEANPELAPAHTALGWLLMEQSRP
                     SEAMEAFNRAVKANPEEAQALYGIGRIYAAKGKRESAAENFSKAIRFEKDPAKKNAIM
                     NALHASG"
BASE COUNT          587 a          801 c          794 g          508 t
ORIGIN      
        1 tgcgggcaag tcaacgcgat gagcggttcc atctcatgag cgacggcgct gttgcaccct
       61 tttcgctgcg ggcgtatagt gccctccttg taaaaatggc atggcgctgc tgccgcctca
      121 gtacgttcgc ttcacaagac gcccggtctc atacttcaag cctatgacga cttcagcccg
      181 ccgcgtgatt gccctggctc cgatgcctcc ggagaaatcc gcctatgccc tcgcgcggta
      241 tagccggtct ccggactcga ttgaagacag catcaagtgg gtccacggcc attcatccga
      301 gaaattctgg gaacagtttt atttcgacta tgggcatggt tcgatcgcgg acctcggcca
      361 cgtcattatc tgcttcgagc agatttccga actcgccgcc atccggttgg aggatgaacc
      421 ggtgtgggat ggccaggcca agtcgagccg ctatcagaat ttcgccacct ccagctggtt
      481 tgtcccggcc accattcgag gacaggagat tgaggcgagt tatctgggca ttctccgcgg
      541 gctcgcgacg gtctatcgcg ccttgcatga cccgttgagc cagttcctca ccgagcgtga
      601 gccgcgaccg gagagcatga cgcctgctgc ctaccagcgg gctatcaccg cgcgggcctt
      661 cgatgtcaca cggtatttac tccccttggc ggcgcagacc aatgtggggc aggtcgtcag
      721 catcaggacg ctcgaaaaac agatcacccg cctcttgtcc tcgcagatac cggagttgcg
      781 ccaaatcgga gaagatttgc aggaagcctg tcgcaagccg ccggtcaatc tgtggggtga
      841 gttgtcgggt cagtcggccg gattaggtga gccgatggct ccgaccttgg cgcggtacgc
      901 gaagccgagc gtctaccagg ccgaagtgta cagtgacctg gctcgctacg cgaaggacgt
      961 gctgaagggc accggcttgg atcaaccctc cgcctatggg gccgcggaac cagtagattt
     1021 gatcgaacct cacgacccgt tggacgaagt ggtgaccacg ctgctctatc gcgcctccca
     1081 cgctccgtat cgaaagattc tcgccctcgt ccaagactgg accgagaaac aaaagcagga
     1141 caccttggaa gtcgcgtttc agaaacgcgg gccgtatgat gaattgatca aggaattccg
     1201 cagcgggtat gcctttatct tcgacgtcat gatggatatc ggcggctggc gcgacatgca
     1261 tcggcaccgc cgctgccaac aggtgcagca gaatttcacg acggtgcatg ggttcgaaac
     1321 accgcccatc ctcgcagaag ccgggctcga acaggaatac cgagaggcca tgggacatgt
     1381 gaaatcggac attgagcggc tgaagaaatc cagccaggaa gccgcgctgt atgccattcc
     1441 attcggattt tccgtgcgct gtctgttcaa gatggactat gccgaggcgg aatacatcgc
     1501 gaagttgcga tccggcgtga aggggcactg gtcctatcgc accatcgcct ggctgatgaa
     1561 agaaaaaatg accgcacggt atcccatcct cggcgcgcgc atgcaggcga ccccgccgga
     1621 tgtgcaggat gcattgacgc gctgaccggc gcaccgaacc gtctcatgaa tcagcctctc
     1681 aaccagatcg aaacggccct ccttgcgggc gactgggacc ggttagggta tgaagccggg
     1741 gtctcggttc gtgcgctcga agcggccgac gaaaaagacc cccggccata ttttgcgttg
     1801 aatgtcacac acctgattcg cggtgaattt gccgaggcct ggcgggtgca tgcccatgca
     1861 ttgcaggaaa cagcggatat tgagaaggtg ggcgaatggg taaaagccgt cctgaagcag
     1921 tatcccgaca atccctacct gaacctggta caaggcatgt ttctggcgca gtccggccag
     1981 tcggaacaat cggtggtgtt ctataaagaa gcggcgcgcc ttgcgccaca gtccgcctat
     2041 ccccattatt ttctggctca gatccatgag cgggcgaacc atctggaaat ggccatcaag
     2101 gagtaccgcg aggcggtcaa actggatccg gcattcgccg cggcccgcac caacctgggc
     2161 gtcgcctacc aggagcaggg ccggctcgaa atggccattc ctcaatatcg cgaagtgatc
     2221 aagctcaatc cgaacgacgg cgtcgcccat gcgaatctcg gctgcgcctt ggcggagcag
     2281 gggaagtttg aaccggcgct ccaggcctac aaggaggcgc tgcgcctcaa tccgaacgac
     2341 gcggagattc acttcgcatt gggcggagtc tacgaaacga aaggccgcat ggatctcgcg
     2401 gtcaaggaat accgcgcggc cacggaagcc aatccggaac tcgcgccggc tcacaccgcg
     2461 cttggctggt tgttgatgga gcaatcccga ccgagtgagg ccatggaggc cttcaatcgc
     2521 gccgtgaagg ccaaccccga ggaagcgcaa gcgctctacg gcatcgggag aatctacgcc
     2581 gctaagggca aacgggaaag tgctgccgaa aacttctcca aagccatccg cttcgaaaaa
     2641 gatcccgcca agaaaaatgc catcatgaac gctctccacg ccagcggtca
//
LOCUS       DAOQEW010000353         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_19998_length_2683_cov_4.744673, whole genome shotgun sequence.
ACCESSION   DAOQEW010000353 DAOQEW010000000
VERSION     DAOQEW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19998_length_2683_cov_4.744673"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1003)
                     /locus_tag="PLS35_19905"
     CDS             complement(251..1003)
                     /locus_tag="PLS35_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20993.1"
                     /translation="MRQVVCWLDQRGQDVQLHTHPEVLPKSFWTDNGVEYRPEDMNDY
                     SNDAWAEFVISHFSKLISDVTGKDILAYRAGSFRWNACSIRALKAVNIPMSFNNSMRA
                     YREGRCVFSEPTNLPYAWSNGMIEVPVTEKRMPIQPGGRECWTSLTYPEAAYFPFQPE
                     RASVISRIFGRGLTFCVFLIHSWSLLSWDENGHATYRDDQRLEGYRKLLARLACDYDI
                     ITTRDFLDLYARGKITLSQTVDISKADLKRYA"
     gene            complement(1427..2383)
                     /locus_tag="PLS35_19910"
     CDS             complement(1427..2383)
                     /locus_tag="PLS35_19910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNI20994.1"
                     /translation="MVTLFSLSCRLLAVDFYLATNGDDGNDGQSIARPVATLKRAIEL
                     SRNKVKSEEDAVRIFVQPGTYVGQTIVLESSDTKGRLAIIGQGKKPDEFPIFAGAGNI
                     ATWFTLRGSQGRDSGLTIEALKIRDYFTAVSLEGDRDDKRRGNAGTTIRRNVFQNIGS
                     VAVSENASSTAAIRLVNSHSNIIENNYFGRIRNRQDCGSLHAIYVAHFSSGNVISNNT
                     FDDICGSVIKLRDRSNDNIIRNNRFAHLERVSAIEEWFCDMADRQDCTKKLGECPSTG
                     NAADGNSISESVQPTMISINGNHERRPWCLAEDFEKERVVTR"
BASE COUNT          638 a          796 c          623 g          626 t
ORIGIN      
        1 acagatccac aaagaacacg tgcctggctt tgaactcgtc gccgagcgag cacatctccc
       61 gaataccagc cgacccattg tcatggacac cccaaatcag gcgtctgaca tggtcgtgcg
      121 aggcacgctt tggcaaggct tccgtatcaa ccgtcaagag cgcatagcgg ttcgccatcc
      181 cgtattccca tttcgtgcgc gttgcccccg ggaagtcccc gactcctggc ctcggacggg
      241 aaaaacaacc tcaggcatac cgcttcaagt ctgctttact gatgtcgacc gtttgcgaga
      301 gcgtgatctt gccccgcgca tacaggtcga gaaaatccct tgtggtgatg atgtcgtagt
      361 cgcaggcaag ccgagccaac agctttcggt acccctccag gcgctgatca tcccggtaag
      421 tggcgtggcc gttctcgtcc caagacagca gggaccacga atggatgagg aaaacacaga
      481 atgtcaaacc tctgccaaaa atcctcgaaa tcaccgatgc ccgctccggc tggaacggga
      541 agtaggctgc ttccggatag gtcaaacttg tccaacactc tcttcctccg ggctggattg
      601 gcattcgctt ttcagtaacc ggcacctcga tcattccgtt cgaccaggcg tagggcaaat
      661 tcgtcggctc gctgaaaacg caccgtccct cacggtaggc gcgcatcgaa ttgttgaaag
      721 acatcggaat gttgaccgcc ttcaacgcac gaatggaaca tgcattccag cgaaacgatc
      781 cggctcgata cgcgagaata tccttgcccg taacatccga aatcagtttg ctaaaatggc
      841 tgatcacaaa ctctgcccaa gcatcgttgg agtagtcgtt catgtcctcc ggccgatact
      901 ccacgccatt gtccgtccag aaactcttgg gcaatacctc cggatgcgta tgcaactgaa
      961 catcctgccc tcgttggtcc agccagcaga ccacctgccg catttcctcc ggataccgat
     1021 aggcgccgca catatcgaca aaaaacacat gtttgacacc aaattcgtca ccgatcgcgc
     1081 acatttcact gatgccggct gtgcccctct catgctttcc ccaaatcagc cgattcacat
     1141 gatcctggag tgcccttttg ggcaaagcct ccgtgtctac agtaagtatt gcatatcgct
     1201 gtcccaattt catcggtcgc caccactcac catatcatgc cgcggcacga aaagcatgtc
     1261 gaacgtgcca gccggacccg atacctgttc cttaacattg cgatgcccta gtcgcatgtt
     1321 tcaccaatgc ccatacggat cgaatatgac ggctccagcc aagcaaggaa ttctgcgtga
     1381 caaaaacgca aacgattgaa gtccgaaagt ctgctaaccc tggtcctcag cgtgtaacga
     1441 cgcgttcttt ctcgaaatcc tctgccaaac accagggccg cctttcatgg ttgccattga
     1501 tcgaaatcat cgtcggttga actgactcac taatggaatt cccatctgcc gcattgcccg
     1561 tcgacggaca ttcccccaac ttcttggtgc agtcttgacg atccgccatg tcgcaaaacc
     1621 actcctctat tgcagacacc cgctcgagat gtgcgaaacg attgttcctg atgatattgt
     1681 cgtttgagcg gtcccgcaat ttgattaccg agccgcatat gtcatcgaag gtgttgttgg
     1741 agattacatt acccgaagaa aaatgcgcaa catagattgc gtgaagactt ccacaatcct
     1801 gcctgttgcg aattctccca aaataattgt tctcgatgat gttgctatgc gagttgacga
     1861 gtcgaatggc cgcggtcgaa ctcgcatttt cggaaacagc gacggatccg atattctgga
     1921 acacattgcg ccggattgtg gttcctgcgt taccccttct cttgtcatca cggtcacctt
     1981 cgagtgacac tgcagtgaag taatcgcgaa tcttgagcgc ctcgattgta agaccgctgt
     2041 ccctgccctg ggagccccta agtgtgaacc aagtggcgat attgccggcc ccagcaaaaa
     2101 tagggaactc gtctggcttc ttcccttgac caatgatcgc caatctccct ttcgtgtcgc
     2161 tactctccag aacgatagtt tggcctacgt aagtgcccgg ttgaacaaaa atgcgaacgg
     2221 catcctcttc cgacttcacc ttgtttctcg agagttcgat tgcacgcttt agcgtggcaa
     2281 cgggacgagc tatcgattgc ccatcgtttc catcatcgcc attcgtcgcc agatagaaat
     2341 cgacagccag caaacgacag gataaggaaa acagcgtgac caaaacaagt agtttcttga
     2401 tagcttatct ccggtattta gcatacgaac tcggtgaatt tccaccagat ttccgcaatg
     2461 agttatgaac aatgaatccc ggattgcgcc aaagcctttc gtgttcaaat tcgtactggc
     2521 ttggcggatg acgaacgggc caatcacagg ttcgcattac actgccaatc tccgcagatc
     2581 ccgtacagat attccggatg ctcacgaaac tcgacctccc ggcttgctcg gctccgcgcg
     2641 taaaccgcct cgcgcgtgtc ttgcgttgac catgaacgcg ctg
//
LOCUS       DAOQEW010000354         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_20152_length_2670_cov_5.255067, whole genome shotgun sequence.
ACCESSION   DAOQEW010000354 DAOQEW010000000
VERSION     DAOQEW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20152_length_2670_cov_5.255067"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLS35_19915"
     CDS             <1..422
                     /locus_tag="PLS35_19915"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI20995.1"
                     /translation="AGMCHVMLPAQFGHHRDDAMLKADSTLETLNNRLRDAVEANGHV
                     AVIKSKIFGGAEPPQVGLSFSDGRQSVIFTRTWLRSRNVPILAEWVGGEQRREVVLVP
                     SGGQVFCKRVSMTSDFIELERSGLLTETAPLNKIELF"
     gene            587..1969
                     /locus_tag="PLS35_19920"
     CDS             587..1969
                     /locus_tag="PLS35_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HNI20996.1"
                     /translation="MMGRQTTGREQLFYSFSLEDHVPEDHLLRGIHRFLDLGPFRQHM
                     EAFYSPIGRPSVDPELMIRMLIVGYCFGIRSERRLCEEVHLNLAYRWFCRLGLEDPVP
                     NHSTFSKNRHGRFRNSDAFRQVFEGVLARCMAEGLVRGEGFATDASIIKADVKVNNRV
                     PGNEPIDWGNPDEATRPVREYLAALAEVNDPPPPFKAVSLTDPAASWTNRGGPSFFAY
                     STNYLIDLKAGIIVDVEASAVSKAAEVTATRKMIERVEDRFDLKPERLVGDTNYGSAA
                     LLGWLVDEKQIEPHVPVCDKTERQDGTLSASAFAWHVQNNEYRCPAGKPLRSDWRSFN
                     NPRTHITLADTIIYRARQPDCAACQLKSQCCPNMPSRKIHRSIHEDARNVARQIATTP
                     EYEQSCRERKKVEMLFAHLKRIMKLDKLRLRGFSGANDEFLLAAATQNLRRMAKWLMP
                     SADKANLLPA"
     gene            2371..>2670
                     /locus_tag="PLS35_19925"
     CDS             2371..>2670
                     /locus_tag="PLS35_19925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNI20997.1"
                     /translation="MPENRRNATRERILDTAETLFMAQGYEGTSMRQITGQAQVNLAA
                     VNYHFGSKEELLQEVFRRRLKWLNDERLRVLDALEAEAGGHALKPSQIVDAFFGTA"
BASE COUNT          563 a          774 c          776 g          557 t
ORIGIN      
        1 gggccgggat gtgccacgtc atgctgcctg ctcagttcgg acaccatcgt gacgatgcca
       61 tgctgaaggc tgacagtacg ctagagacgc taaacaatcg attgcgcgat gcggttgagg
      121 cgaacggcca tgttgctgtc atcaagagca agatatttgg gggagccgag ccgccgcagg
      181 ttgggctatc tttttcggat ggaagacaga gtgtcatctt tacacggacc tggctgcgat
      241 cccgcaacgt tcccattctg gccgagtggg ttggtggtga acagcgtcgg gaggtcgttc
      301 ttgttccaag cggcggacaa gtcttttgta agcgggtatc aatgacatcg gatttcatcg
      361 aacttgagcg ttctggtcta cttacggaga ctgcgccgtt gaacaaaatc gaactctttt
      421 agcacgctgc tattgaggtt cattggccga attctgatca aaacagttcg attcatctga
      481 ctggcggctg aacgacagat gactggctaa ttctgttgaa aaaggcctgt caatgacacc
      541 ggcagtggcg gtatgatgat ggcattgtgt gatcgggtgc cacgccatga tgggacgtca
      601 aacaacgggt cgggaacaac tgttctacag cttcagcctg gaagatcatg ttcccgagga
      661 ccatctgctg cgggggattc atcggtttct tgatctgggg cccttccggc agcacatgga
      721 agcgttttac agccccatcg gacggccgtc agttgatccg gaactgatga ttcgcatgct
      781 gatcgtcggc tactgcttcg gaattcgttc agagcggcgt ttgtgcgagg aagtgcatct
      841 caacctcgcc tatcgttggt tctgccgact gggtctggaa gatccagtgc cgaaccattc
      901 cactttctcc aagaaccggc acggtcgctt ccgcaatagc gacgccttcc gtcaagtgtt
      961 tgagggtgtg cttgcccgtt gcatggcgga agggttggta cgcggcgaag gttttgccac
     1021 ggacgccagc atcatcaagg ccgacgtgaa agtgaacaat cgtgttccgg gaaatgagcc
     1081 gattgactgg ggcaacccgg atgaagccac acggccggtc cgagagtatc tggcagcgct
     1141 ggcggaggtc aatgatccgc ccccaccgtt caaggctgtc tccctgacgg acccggccgc
     1201 ctcctggacc aatcgcggcg gaccgtcctt cttcgcctat tcgaccaact atctgatcga
     1261 tcttaaagcg ggcatcatcg ttgacgttga agcctctgcc gtcagcaaag ccgccgaagt
     1321 cacagccact cgaaagatga ttgagcgggt agaggacaga tttgacctga agccggaacg
     1381 cctggtcggt gataccaatt atggatcggc cgcgctgctc ggctggctgg tcgatgagaa
     1441 acaaatcgaa ccccatgtgc ccgtgtgtga caagacagaa cggcaggacg gcaccctatc
     1501 ggccagtgcg ttcgcgtggc atgtgcagaa caacgaatac cgttgcccgg caggcaagcc
     1561 actgcgttcc gattggcgtt ccttcaataa tccgcgcacc catattacgc tggcggacac
     1621 gatcatctac cgcgcgcgcc agcctgactg cgcggcgtgc cagctgaaga gccagtgctg
     1681 cccgaacatg ccctctcgca aaatccaccg cagcatccac gaggatgccc gcaacgtggc
     1741 ccggcagatc gccaccactc ctgaatatga acagtcatgc agggaacgca agaaagtcga
     1801 gatgctgttc gcccacctca agcgcatcat gaaactggac aagttgcgat tgcgcggctt
     1861 cagcggggcg aatgatgaat ttctgctggc ggcagcaacc cagaatttgc gcagaatggc
     1921 gaaatggctg atgccctcag cggataaggc aaacttgctt cctgcctagc ggcaggtaaa
     1981 ggccctctgg cctcctcggc ctcaccgcca acgtcatcaa ccgtctgttt cagtagcctc
     2041 gacccactac gcatcgcttc caaagccgac tttttcaaca gaatttggct ctttgctgcc
     2101 cgatactgcc tgcctcagca gcggcggctc aggccgagtt gttcgcttag accacgtggc
     2161 aggtttcaga gcaaagcggc agctcggata tctcctccag agaagcgcgc cgtcccccta
     2221 tattcagtag cagcgacgtc ggcacccggc gcgaggcgtg gtgtttcatg cccccgggcg
     2281 gtcgcgccca cggcgaaagc cgcgaaagcc gcttgccagc cccgctcggc tgttctacga
     2341 ttgaaacaaa cgtttgaatt tggtcgtccc atgccggaaa accgtcgcaa cgctacccgg
     2401 gagcgcatcc tcgataccgc cgagaccctg ttcatggccc agggttacga ggggacgtcg
     2461 atgcgccaga tcaccggtca ggcccaggtg aatcttgcgg cggtcaatta ccacttcggt
     2521 tccaaggagg aactgctgca ggaggtcttc cgccggcgcc tcaagtggct taacgacgag
     2581 cgcctgcggg tgctcgatgc ccttgaggcg gaagccggcg gacacgccct gaagccttcc
     2641 cagatcgtcg atgccttctt cggcaccgcg
//
LOCUS       DAOQEW010000355         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_20414_length_2651_cov_8.605162, whole genome shotgun sequence.
ACCESSION   DAOQEW010000355 DAOQEW010000000
VERSION     DAOQEW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20414_length_2651_cov_8.605162"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLS35_19930"
     CDS             <1..1262
                     /locus_tag="PLS35_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNI20998.1"
                     /translation="SNVPAVPAPVAAATEGTSAPPVASTGTTQLSFRTILRDQNQNHV
                     LEQNERFSVEFEVRNDGPTPADAVAVDLSGHPAIIASVKTPVSVGLLQPGEIRRVTVE
                     GIVGKVAEAEQAELICTLQAAANVALPSAKKFFVAIRPDATDATEVVSVDVDQLPSKK
                     GKPVQPHAVAIAIGVGAFRDPAMPTMKLATHDAEIMSGYFTSVMGISPQKVKVVLDAK
                     GLKDDWIEVFEQWLPKQVDPQTTAYVYVAGRAMVDHETGAVSLLPYDATLSGSSRAFS
                     LARLQRALTNAPVKQAVVMLDLSLEPSSGSDPARVAQPRWISPEANGEQERVMLMVGN
                     ATVQEAQAYQPGQHGLFTYFLLKGLRGAADLDKNGSVLTGELCAYVQRQVTAVGRSTS
                     GDTQQPLCLPDANGASPLRQLRLSKLP"
     gene            1363..2523
                     /locus_tag="PLS35_19935"
     CDS             1363..2523
                     /locus_tag="PLS35_19935"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNI20999.1"
                     /translation="MRIRSLANLLVITVLLLISVGLSVALLEFAAGYLYDRAGLANGK
                     RMVDLYLGRTPEMRVGAKEAEKASITPHPYLLFVHTPNMTVDGYQQTNSLGYRNKEFT
                     VEKPADTIRILVVGGSTTFMWPYIKNPADTWVARLEAKLQAISSKQVQVINAGVNYGT
                     SAEALAGYVFRHRFLQPDIVIYHGGGNDVLPLFFDHYNPEYTHFRSAGGSKPRPGEKR
                     LLEHSHTARYLYARWLEPVGSVVVTQPFWEVDPLVALERVRQQAPIGFERNVDSLITL
                     SKSSGADVVLFGFLQAKKQYLSKNAPAFKGYEDALVLGLEKNYEVLARAGSRHHVPFV
                     IPAQDRFQDEWFQDNCHLTPEGEEVKAQIMFEELKTHPQFFSARNPTPPVTH"
     gene            2568..2643
                     /locus_tag="PLS35_19940"
     tRNA            2568..2643
                     /locus_tag="PLS35_19940"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2601..2603,aa:Thr,seq:cgt)
BASE COUNT          574 a          729 c          788 g          560 t
ORIGIN      
        1 tgtccaatgt gccggccgtt ccagcgccgg tggcagctgc gactgaaggg acctctgcac
       61 cgccagtcgc ctcgaccggc acaacgcaac tgtcattccg caccattttg cgagaccaga
      121 accaaaacca cgtcctggag cagaatgaac gcttcagcgt ggaattcgaa gtcagaaacg
      181 acgggccgac gccggctgac gccgtggcgg tggatctcag cgggcatccg gccattatcg
      241 cctcagtgaa aacgccggtc tccgtggggc tgttgcagcc gggggaaatt cggcgcgtca
      301 cggtagaggg cattgtcggt aaggtagcag aggcggaaca ggcagaattg atctgcaccc
      361 tgcaggctgc cgcgaacgtt gcgcttccgt cggcaaaaaa gttttttgtg gccatcagac
      421 cggatgcgac cgatgcgacc gaagtcgtct ccgtggatgt cgatcaactc cccagcaaaa
      481 aggggaaacc ggttcaaccg catgccgtcg cgattgcgat cggagtcggt gcgtttcgcg
      541 atccggccat gcctacgatg aaactggcca cgcacgatgc cgaaatcatg agcgggtact
      601 tcacgtccgt gatgggaatt tcgccgcaga aggtcaaagt tgtgctggat gccaagggac
      661 taaaagacga ttggattgag gtattcgaac agtggttacc caagcaggtg gacccgcaga
      721 ccacggccta cgtgtatgtt gctggacgag caatggtgga tcatgagact ggtgccgtat
      781 cgctgctacc gtatgatgcc acgctatccg gctcttcccg cgcgttctct ctggcccggt
      841 tgcagcgcgc actgacgaac gcgcctgtca aacaagccgt cgtgatgttg gatctctcac
      901 tggagccctc gtccggttcg gaccctgcgc gcgtggccca gccgcggtgg atttcgccgg
      961 aggccaacgg ggagcaggag cgtgtgatgc tgatggtggg caatgccacc gtgcaggaag
     1021 ctcaggcgta tcaaccaggc caacatggcc tcttcacata ttttctcctc aagggacttc
     1081 gcggcgcagc tgatctcgat aaaaacggat cagtgctcac cggagaattg tgcgcctacg
     1141 tgcagcgaca ggttacggcc gttgggcggt cgacatcggg ggatacccaa cagccgctct
     1201 gccttccgga cgccaacggc gcgtctcccc ttagacagct ccgtcttagt aagctgccct
     1261 gacggcgcgt caccggttcc aatagtggtc tagcgtactg aacgattcat tcgtggttcg
     1321 tccggtcctt cgctctctgg ttgcttgtgc tcgcggcaca ccatgcgaat acgaagcctc
     1381 gccaatcttc ttgtcatcac ggtgctgctg ctcatcagcg ttggcctatc cgttgcgctg
     1441 ttggaatttg ccgcgggcta tctctacgac cgcgcgggac tggcgaatgg aaagcggatg
     1501 gtggatctct atctaggccg gacgccagag atgcgtgtgg gtgcaaaaga agcggagaag
     1561 gcctcgatca cgccgcatcc gtatctgctg tttgtgcata cccccaacat gaccgtagac
     1621 gggtaccaac aaacgaattc actcgggtat cgcaataaag aatttacggt ggagaaaccg
     1681 gccgacacca ttcggattct ggttgtgggc gggtccacca cgttcatgtg gccgtacatc
     1741 aaaaatcctg ccgacacatg ggtggcgcgc ctggaagcga agctccaagc gatatcaagc
     1801 aaacaagtcc aggtcatcaa tgccggagtc aactatggca cgagtgcgga agccttagcc
     1861 gggtacgtgt tccggcatcg gtttcttcag ccggacatcg tgatttacca tggcggagga
     1921 aacgatgtgc tgccgttatt ttttgaccat tacaaccctg agtatacgca tttcagaagc
     1981 gccggtggga gtaagccgag accgggagaa aagcggctcc ttgagcacag ccataccgcc
     2041 agatatcttt atgccaggtg gcttgaaccg gtcgggtcgg tggtggtgac gcaaccattt
     2101 tgggaagtgg acccgctcgt cgcattagaa cgggttcggc agcaagcccc gatcgggttt
     2161 gagcgaaacg tggattctct gatcactctg agcaagagtt caggggcgga tgtggtgctc
     2221 tttgggtttt tgcaggcgaa gaaacaatat ctgagtaaga atgccccggc atttaaaggt
     2281 tatgaagatg cgctcgtact tgggctggag aaaaattatg aggttctagc acgggccggg
     2341 agtcggcatc acgtgccctt tgtcattcct gctcaggatc gattccagga tgagtggttt
     2401 caggacaatt gccacctgac accggaagga gaagaggtca aagcgcagat catgttcgag
     2461 gagctgaaga cccatcctca atttttcagc gcaaggaacc caacaccccc cgtcacacat
     2521 tgacaggtgt ttcaccgtct gagtaatatt gcgcctcacc ccgcctggcc ggcgtagctc
     2581 agttggcaga gcagcggttt cgtaaaccgc aggtcggtgg ttcgattcca ctcgccggct
     2641 ccatttatca a
//
LOCUS       DAOQEW010000356         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_21348_length_2586_cov_9.420782, whole genome shotgun sequence.
ACCESSION   DAOQEW010000356 DAOQEW010000000
VERSION     DAOQEW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21348_length_2586_cov_9.420782"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PLS35_19945"
     CDS             <1..1206
                     /locus_tag="PLS35_19945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNI21000.1"
                     /translation="SATPGVLIHMAALDNLLHGQGLQAAPSWFSLSILLLLCLVSAGA
                     FMLFPSYPVKFGVTIGLALAYYGLVAHAFAGHERWLDLVFPEVALALTFGTAATVEYV
                     TEGKQRRLMRAAFDKYMSSEVVEEIMRNPAAIKLGGEKKEISILFSDIAGFTTISEKM
                     SPEDLVALLNRYLSAMTTIIKTTHRGNVNKYLGDGIMALFGAPLNDPQHASLACYAAL
                     DCQVELARLRQVWKQEGLPEIGARIGINSGACIVGNMGSEERMEYTVTGDSVNLASRL
                     EGASKYYDTLILIGQRTAELAKNDVEVREIDLLRVKGKKDPVVVFELLARKGQVEEKK
                     RRVIDVYLEGLAAYKVRNFSIACAKFSEAVTLDPSDGPSRVYLERATNYCQTPPSADW
                     DGVYEMTSK"
     gene            1233..1745
                     /locus_tag="PLS35_19950"
     CDS             1233..1745
                     /locus_tag="PLS35_19950"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNI21001.1"
                     /translation="MISPRVLRVVLIVLWIGLLVWSGEGRAETVYVQAKTAQLRAGKT
                     SLDAVVGNVKFGDALEVVGRDGSWVEVKTSAGAKGWIFANKTSSSKPSGTNDTLAKLG
                     QSMRGSDASATTASAGARGLDKASEGYANRAGVSARDRDAVDRMTAYQIPDQEVEEFL
                     REGGLGEYAK"
     gene            1735..>2586
                     /locus_tag="PLS35_19955"
     CDS             1735..>2586
                     /locus_tag="PLS35_19955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNI21002.1"
                     /translation="MRSNHGLTATIIALSLMVAGCAEVQRASEDIARQSGNPRLAGAI
                     HGVGNVVGSLFPIGYEEESSIGQAMALQVVARYGGVVDQPELVRYVNLVGRAVANTSD
                     RPDIPYRVAILEHESINAFAAPAGYIFVTRGLLKQVRNEAELAAVLGHEIAHVSQKHI
                     LDVIQRSKRLAGVTEAGLSYATSNPAAFKTVIDGAVKKLLDEGLDQDKETEADTIGEV
                     FATRVGYDAEAYVNLLTRLRDLKGDDRALFKTHPNFSARIEAVQKTIQSKHLVATGVL
                     LPERFARM"
BASE COUNT          528 a          697 c          833 g          528 t
ORIGIN      
        1 tccgccacgc ctggcgtgct catccacatg gcggcgctgg acaacctgct gcatgggcag
       61 gggcttcaag cggcgccgtc ctggttttct ctttccatcc ttctgctgct ttgccttgtc
      121 tcagccggtg ccttcatgtt gttcccgtcc tatccggtca aattcggcgt cacgatcggc
      181 ctcgctctgg cctactatgg gttggtggcg catgccttcg cggggcatga gcggtggctg
      241 gatctggtgt ttccggaagt cgccctcgcc ttaacgttcg gcacggccgc gacggttgag
      301 tacgtcaccg aaggcaagca gcgccggctt atgcgggccg cgttcgacaa atacatgtcg
      361 tctgaagtgg tcgaagaaat catgcggaat cccgcggcca ttaagcttgg tggcgagaag
      421 aaggagattt cgattctctt ctccgacatc gccggtttca cgaccatctc cgagaaaatg
      481 tcgccggagg atcttgtcgc attgctgaac cggtacttgt cggcgatgac gaccatcatc
      541 aagacgacgc atcgcggcaa tgtgaacaag tatctcggcg atggaatcat ggcgctcttc
      601 ggcgcgcccc tgaacgatcc ccaacatgcg tcgctggcct gttatgccgc gctggattgc
      661 caggtcgaat tggcccggct tcgtcaggtc tggaagcagg agggacttcc agagatcggc
      721 gcccgcatcg gtattaattc cggggcctgc atcgtcggca acatgggatc ggaggagcgc
      781 atggagtaca cggtgaccgg ggacagcgtg aacctggcct cacgcttgga gggcgccagc
      841 aaatattacg acaccttgat tttgatcggc cagcgaacgg ccgaattggc caagaacgac
      901 gttgaagtgc gagagatcga tctgctacga gtgaagggca aaaaagaccc ggtcgtcgtc
      961 tttgagttgc tggcccgcaa ggggcaagtg gaggaaaaga agcggcgcgt catcgacgtc
     1021 tatttggaag gattggcggc ctataaagtg aggaacttct cgatcgcctg cgccaaattt
     1081 tctgaagcgg tgaccttgga cccatcggac ggtccgtcgc gggtgtatct cgaacgcgcg
     1141 actaattatt gccagacgcc tccgtcagcg gattgggacg gggtctatga aatgacatca
     1201 aagtgagcgg cggtgttgaa ggggaggaag ctgtgatatc accacgagtg cttcgcgttg
     1261 tcctgatcgt tctgtggatc ggtctcctgg tctggagcgg agaaggccgg gccgaaaccg
     1321 tctacgttca agccaagacc gcacaactgc gcgccggcaa aacgtcgttg gatgctgtcg
     1381 tcgggaacgt caagttcggc gacgcactgg aggtggtggg gcgtgacggc agttgggtgg
     1441 aggtcaagac atccgctggc gcgaaaggat ggatcttcgc caacaaaacc tcgtcgtcca
     1501 aaccgtccgg caccaatgat accctggcca aactcgggca gagtatgcgg gggagcgatg
     1561 cgtcggccac gacggcgtcg gcaggcgcca gaggcctgga taaggcttcc gaaggctatg
     1621 ccaatcgcgc cggcgtatcc gcgcgcgatc gtgacgccgt ggaccggatg accgcgtatc
     1681 agattcctga tcaggaggtc gaagaatttt tgcgagaagg aggcctcggt gaatatgcga
     1741 agtaatcatg gcctgacagc gacgatcatc gccctctcac tgatggtggc cggttgcgcg
     1801 gaagtgcagc gcgccagtga agatatcgcg cggcaatccg gtaatccgcg gctggccggt
     1861 gccattcacg gagtcggcaa tgtggtgggg agtttgttcc cgatcgggta cgaggaagag
     1921 tcgtcgatcg gccaggcgat ggctttgcag gtcgtcgcac ggtatggcgg agtcgtggat
     1981 cagccagagc tcgtccggta tgtgaatctg gtgggacggg ccgtcgcgaa cacgtctgac
     2041 cggccggata ttccgtatcg ggtggcgatt ctggaacatg aatccatcaa tgcgtttgcc
     2101 gcgccggccg gctacatctt tgtgacgcgt ggtctgctta agcaggtccg caatgaagcg
     2161 gaattggctg cggtcctggg ccatgaaatc gcccacgtca gccaaaagca tattctcgat
     2221 gtcattcaac gcagcaaacg cctggccggg gtaaccgaag ccggcctgtc ctacgcgacg
     2281 agcaatccag cggcgttcaa gaccgtgatc gacggcgcgg tcaagaagtt gctcgatgaa
     2341 ggcctggatc aggacaagga aacggaagcc gatacgatcg gcgaagtgtt tgcgacgcgg
     2401 gtcggctacg atgcggaagc ctatgtgaac ttgctgacgc gcctacgaga cctcaagggt
     2461 gacgaccggg ctttgtttaa gacccatccc aacttttcag cgcgtatcga ggccgtgcag
     2521 aaaacgattc agagcaaaca tctcgtggcc accggcgtct tattgccgga gcggtttgct
     2581 cgcatg
//
LOCUS       DAOQEW010000357         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_21714_length_2561_cov_5.440144, whole genome shotgun sequence.
ACCESSION   DAOQEW010000357 DAOQEW010000000
VERSION     DAOQEW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21714_length_2561_cov_5.440144"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLS35_19960"
     CDS             <1..320
                     /locus_tag="PLS35_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21003.1"
                     /translation="VYAMGFPNANCIPCGKATSPAYWALVRQEFPVQFHRMAGLCRDV
                     GARLTRIDDVRMFIDEIPEGHPVTEAIAPECDFLCSLAEQDLTPSNVELTGAARHERK
                     TKP"
     gene            705..989
                     /locus_tag="PLS35_19965"
     CDS             705..989
                     /locus_tag="PLS35_19965"
                     /inference="COORDINATES: protein motif:HMM:NF028096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family recombination enhancement nuclease"
                     /protein_id="HNI21004.1"
                     /translation="MSKSSRHMARVAALGCLLCRRLGYLGTPAEVHHIRTGTGAGRRA
                     KDTETIPLCPEHHRGDTGLHGMGRKAFERAYRVTELELLAETRAMIGDRG"
     gene            1233..1733
                     /locus_tag="PLS35_19970"
     CDS             1233..1733
                     /locus_tag="PLS35_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21005.1"
                     /translation="MAARKGKKTTVEVQKRPRGAPSTYSVARAKTVLDQIACGVTLAE
                     ICRQPGMPGYTTVWEWKNAHPEFAEAYARAREVGFDVLAEGCLAIADDGTNDYMQEAT
                     GDGAAAYRLNGEHVQRSKLRIWTRLELLKRWDPKRYGELTKIDMTVTNPLAERLARAK
                     ERLDGK"
     gene            1720..>2561
                     /locus_tag="PLS35_19975"
     CDS             1720..>2561
                     /locus_tag="PLS35_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HNI21006.1"
                     /translation="MTESDITDQIIDLAAECATDPVRWVAAAYDWGVGELAAHQGPRA
                     WQDDILGEIRDHLSNPATRFEPLQIAVSSGHGIGKSALIGMLANWGLSTSEYCKVVCT
                     ANTDTQLRTKTSPEVGKWARMSITADWWDVQATSIAARDRGTAKDWRMDFVPWSETNT
                     EAFAGLHNQGRRIILIFDEASAIADKVWEVAEGAMTDADTEIIWLACGNPTRNTGRFR
                     ECFRRFRHRWITRQIDSRDVEGTNKTQIEKWVEDHGLESDFVKVRVRGMFPSMSAKQF
                     ISEA"
BASE COUNT          576 a          721 c          856 g          408 t
ORIGIN      
        1 gggtgtatgc gatggggttc cccaacgcga actgcattcc atgcggcaag gcaacaagcc
       61 cggcgtattg ggcactggtg cggcaggagt tcccggttca atttcaccgc atggccggat
      121 tgtgccgtga tgtcggggcg cgactcaccc gcattgacga tgtgcggatg ttcattgacg
      181 agataccgga gggccatcca gtgactgagg ccattgcacc ggaatgcgac tttttgtgca
      241 gcctagcaga gcaagacctg acgccctcta acgtagagct aaccggcgct gcgcgccacg
      301 aaaggaaaac gaagccatga gcgaccagac acagacaacc tcaacaacgc ctgatggagc
      361 agcgtccgtg ttgagcgcca ggttagcgcc cacaacgcta tgctggaatt gcaacacagt
      421 gtatccgatg gaagcgcacc aatgccccgg atgctgcgcc acgaacgcca acaaagacct
      481 tgcgagcgcg caggaagaaa tgcaggacaa gcgcctgatt gaccacgatt ggaagtgggt
      541 aagcgattgg tacggcgatc ctgacgtagt gaacggaacc gccgattgct cgcactggca
      601 atgcacgcga tgcgactcgg aggactgcga agatgaaccg cccggccctt gcgatgacta
      661 ctgacatggg cgctaacgca aattatccgg agggtgcaag gtagatgagc aaatcctccc
      721 gccacatggc ccgagttgct gctctcggat gcctgctctg ccggcgcctc ggctacctcg
      781 ggacgcccgc cgaagtgcat cacatccgca cgggcaccgg agccggccgg cgggcgaagg
      841 acaccgagac tatcccgctg tgcccggagc atcaccgggg ggatacgggg cttcatggca
      901 tggggcggaa ggcgttcgag cgcgcctatc gcgtcactga actggaactg ctggcggaaa
      961 cgcgggcgat gatcggggat aggggatgag tccgccgagg atcatccgcc tggtgggaac
     1021 gaacgaccgt ggggttgcca tcgggcagta tcacccgagg gctaaatact ccgacgaact
     1081 gatcgaccag atccgtgaga tgcgggaaga ccacaagctg cccacgcgag aaatagctgc
     1141 ccggaccgga gtttcgcgga gaatggtcag ggatgtgtgc gcgtatgttt accgggcgca
     1201 gacatacacg aactggagaa aggctggagg aaatggcggc gcgaaagggt aagaaaacga
     1261 ccgtcgaggt tcagaagcgt ccgcgcgggg cgccatcaac ctattccgtt gcgcgggcga
     1321 aaaccgttct cgatcaaatc gcgtgcgggg tgacgctcgc ggaaatttgc cggcaacctg
     1381 ggatgcccgg atatacgacg gtttgggagt ggaagaacgc gcacccggag tttgccgaag
     1441 cgtacgcgcg ggcgcgggaa gttgggttcg acgtcctggc ggaaggttgc ctggcaattg
     1501 ccgacgacgg cacgaacgat tacatgcagg aggcgaccgg agacggggcc gcggcatatc
     1561 gcctgaacgg ggagcatgtc caacgctcca aacttcggat ctggacgcgc cttgaattac
     1621 tgaagcggtg ggatccgaag cgatacggcg agctgaccaa gatcgacatg accgtcacca
     1681 acccgctcgc cgaacgcctg gcccgggcaa aggaacgcct tgacggaaag tgacatcacc
     1741 gaccagatca tcgacctggc ggccgagtgc gcgaccgatc ctgtgcggtg ggtcgcagcg
     1801 gcctacgatt ggggtgtagg agaactggcc gcccaccaag ggcctcgcgc gtggcaggac
     1861 gacatcctcg gagaaatccg ggaccacctg tcgaatccgg cgacgcgatt cgagcccctg
     1921 cagattgcgg tctcgtccgg gcatggcatc gggaaaagcg ccttgatagg catgctggcc
     1981 aattggggac tttcaacgtc cgaatattgc aaggtcgtct gtaccgcaaa caccgacacc
     2041 cagctccgca ccaagacaag cccggaggtc gggaagtggg cgcggatgtc catcactgcc
     2101 gactggtggg acgtgcaagc gaccagcatc gcggccagag acaggggcac ggcgaaggac
     2161 tggcggatgg acttcgttcc gtggtcggag accaacacgg aggcgtttgc cggcctgcac
     2221 aaccaggggc gcaggatcat cttgatattc gatgaagcgt cggccatcgc ggacaaggtt
     2281 tgggaagtgg ccgagggcgc catgactgac gccgacacgg aaatcatttg gcttgcgtgt
     2341 ggcaacccga cccgcaacac gggacgattc cgcgagtgct tccggcggtt caggcatcga
     2401 tggatcacgc ggcagatcga cagccgagac gtggagggga cgaacaaaac gcaaatcgag
     2461 aagtgggtcg aggaccacgg cctggagtca gatttcgtca aggtgcgggt gcgcggcatg
     2521 ttcccgtcga tgtcggcgaa gcagttcatt tccgaggccg a
//
LOCUS       DAOQEW010000358         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_21865_length_2552_cov_7.926312, whole genome shotgun sequence.
ACCESSION   DAOQEW010000358 DAOQEW010000000
VERSION     DAOQEW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21865_length_2552_cov_7.926312"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..835
                     /locus_tag="PLS35_19980"
     CDS             413..835
                     /locus_tag="PLS35_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21007.1"
                     /translation="MQFDAALAAQETLQEAQTELGVDWDQATELEETFSSNAGSTARD
                     AYEQLLALAARHPKAHRFQAFCIYITWQQVTEETIARHFQTGMTLAESYLASPDGKDP
                     RHLAHVAELLDSFRAGLGLDEEDDIVVEFRKDTPKGGD"
     gene            921..1688
                     /locus_tag="PLS35_19985"
     CDS             921..1688
                     /locus_tag="PLS35_19985"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI21008.1"
                     /translation="MAENDKHRARIFLHRGDLVQARAAWEAAVKDDRLTGTPRELSNS
                     LGNLGNTLALAGAFEEADACYKEVLAIQRNERDPHAIAHTLVNLGNLHIGADKPEKAR
                     PYYLEALDLLKPLNDHRALGILYHNLALEEARQQQWDESVRLFTQALDSHRVVGNEEG
                     LAGTYSQMGKMFLDSGRSVEAERCFNNATEHFIKLGNPSGEAAVLRELADLYEQRQDT
                     IAAIRCVERLHHLALGTGRAPSTADRDRLTRLRAAHS"
     gene            1754..2071
                     /locus_tag="PLS35_19990"
     CDS             1754..2071
                     /locus_tag="PLS35_19990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI21009.1"
                     /translation="MDISAFRQMVEKNPKGFLGRYGLGNKILQEGGSLEEAAEHLTVA
                     TQLDPTHVASHLALGRALVKLGKKEDAKPVLKAGIDAAVSGRSNGGVDLVPELQQLLR
                     TLG"
     gene            2089..2289
                     /locus_tag="PLS35_19995"
     CDS             2089..2289
                     /locus_tag="PLS35_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21010.1"
                     /translation="MTLDAARQRIESAITQYGQHAAMAIELVINEVRSDMGRDAVNEL
                     IDEFDLELMYNIAPMESDYSNS"
BASE COUNT          576 a          754 c          741 g          481 t
ORIGIN      
        1 agcacgcggg cggaaatggc cgggtccatc ttgaggacct gcgccagatc gtccatagtg
       61 gaatcagggt tgtcgacgac gtcgcggaca cggaaatata tctccggcag ggtaaagaca
      121 tttgagcagg actgaacaag ttcttgcgcc gatgccatga tagacgatcc tccttggtgc
      181 gcgttgcgtg cgccctgcac aggggccccc ggctatcttt atcggtcgcg gcccgtcttt
      241 tctgaagcgc gagggctatt cggcctatac atgaggcctg tttccctccc gatcgacctg
      301 atgaacccgt tccaccgggc cacttctgga tcagcaacat tgacctccct tccgcgcacc
      361 cagtattctg caatcaatcc ggaagcccca catcaatccc gttgaggact gcatgcaatt
      421 cgacgccgcg ttggccgcac aagaaacgtt acaggaagcg caaaccgagc ttggtgtcga
      481 ctgggaccag gccactgaat tggaggagac gttttcctcc aatgccggct cgaccgccag
      541 agacgcctac gaacagcttc tcgcgctggc cgcccgacat cccaaggccc atcggtttca
      601 ggccttctgc atttacatca cctggcagca ggtcaccgaa gagacgatcg cccgtcactt
      661 tcagaccggc atgactctgg ccgaatccta cctggcctcg cctgatggga aggatccacg
      721 gcacctcgcg catgtcgccg aattgctcga ctcgtttcgc gcaggcttgg gactggatga
      781 ggaagacgac atcgtagtgg agtttcgaaa agacacaccc aaaggcggag actaacgtgg
      841 gccgcgagac gtcacccgtg aacgggaaac cgttccggac gcgagagacg agcaacgaga
      901 ggcaacatcg gttctgacgt atggctgaaa acgataaaca tcgcgcgcgc atttttcttc
      961 atcgagggga tctcgtccaa gcccgggccg cctgggaggc cgcggtgaag gatgatcgcc
     1021 ttacgggaac gccacgggaa ctctccaata gtctcggcaa tctaggcaac accctggcac
     1081 tcgccggcgc cttcgaggaa gctgatgcct gttacaaaga agtgctcgcc atccaacgca
     1141 acgagcgcga cccgcatgcc attgcccaca ctctcgtaaa tctgggcaat ctccacatcg
     1201 gcgcggataa accggagaaa gcccgtccct actatctcga agcgctggac ctgttgaaac
     1261 cactcaatga ccatcgagcg ctgggcattc tctaccacaa tctcgcgctg gaagaagccc
     1321 ggcaacagca gtgggatgaa tccgtgcggc tgttcaccca ggccctggat tcccatcgcg
     1381 tggtgggcaa tgaagaaggc ctggcgggaa cctacagtca gatgggaaaa atgtttctgg
     1441 acagtggacg ctctgtggag gcggaacgct gtttcaacaa tgccaccgaa catttcatca
     1501 agctcggcaa cccgtccggc gaggccgcgg tgttgcggga actcgccgac ctctatgagc
     1561 aacggcagga caccattgct gctatccgtt gcgtggagcg gctccatcac ctggccttgg
     1621 gcaccgggcg tgccccttcg acggcagacc gggatcgcct cacacggctt cgcgccgccc
     1681 atagctgaac ggatgggggt gatgctaaaa ttcgcccgtc tcgattgaac aacgatgctt
     1741 cacaaggagt gccatggata tcagcgcgtt tcgtcaaatg gtggagaaaa atcccaaagg
     1801 atttctgggc cggtacggtc tgggcaataa aattttgcag gaaggcggca gccttgagga
     1861 agcggcagag catctgacgg tggcgacgca attggatccg acgcacgtcg cttcacacct
     1921 tgccctcgga cgcgcgctcg tgaaactggg gaagaaagag gatgccaagc ccgtcttgaa
     1981 agccggtatt gacgcggccg tgtccggccg atccaacggg ggcgtggact tggtgccgga
     2041 actgcaacag ttgttacgaa cactcggcta aggccggctg gaggacttat gactctcgac
     2101 gcagccagac agcggattga atccgcgatc actcaatatg ggcagcacgc agccatggcc
     2161 atcgaactgg tcatcaacga ggtccgctct gacatgggcc gtgacgccgt gaacgaactg
     2221 atcgatgagt tcgatttgga actcatgtac aacatcgctc cgatggaatc cgattactcg
     2281 aacagctaaa gcaagatgga gctgaactgc acagcccttc tagggtgctc aaaaaggcca
     2341 tccaacgagg ccgcagacga ggaagacccg gagacgtacg ttcagaggta cgttgaggag
     2401 gattccgagt cgagaacgac gttgggggcc tttttcagca gcctgcgatg ccgttgatct
     2461 ggtgctccat ttccggccac ggctacggcc atgccgcgca agtcgtaccg gtgctcaacg
     2521 cgctcgctcg tctcgtaccc gatctcaagg cg
//
LOCUS       DAOQEW010000359         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_22002_length_2543_cov_6.706190, whole genome shotgun sequence.
ACCESSION   DAOQEW010000359 DAOQEW010000000
VERSION     DAOQEW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22002_length_2543_cov_6.706190"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..792
                     /locus_tag="PLS35_20000"
     CDS             163..792
                     /locus_tag="PLS35_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21011.1"
                     /translation="MRTTVLKGQRGIAWACWLVVLATVWLLPVAPVVAQEAVLEFPIR
                     PFNVDVMLRQQTFAPDDREIGVQLGITAVRYGNVEVRTIYQFFSIHTQDFSTDQHSLF
                     VNPRWNNFIDILDFPKHKPISRIIRHVLFGPLEDRAVPYVGVLAGGVLPGPGNRAPGH
                     LIGGQVGVRFPVAQSLSVDISVQYSQYGVDFRGDAGQAQQWVFLTGIRF"
     gene            762..1823
                     /locus_tag="PLS35_20005"
     CDS             762..1823
                     /locus_tag="PLS35_20005"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNI21012.1"
                     /translation="MGIPDRDTVLIKATWCGRIWAALCCLLLSGCGLLIDAVEQVWPV
                     AGSKVDIICERGRVQVGLAMEPFRPFVFPTIWTDEGARVTGLDVELTRAMTDELSRRC
                     GHPVTPVLHLVRFRDLFRLLNEGHLDWFVSAVATNTPAPSRAGVAYSLPYFYGGGISG
                     ITTSTSVLERVRENLQQQATHPAADRLAATSHALDGLTIAVQDETSAYYYAEANWGAN
                     RLIVCDSLPAAFEYADAKTEPRVDIILGAQPVLEYMVKRVRKDWSLLTRENGRPLFLT
                     KADYGVVVAEESYRLRWLVNDLLLKLDESGRLGEMRTRWLDDEYAFPRRASLEGLPFD
                     VEKMASHYIQGTCRIRPGS"
     gene            1846..>2543
                     /locus_tag="PLS35_20010"
     CDS             1846..>2543
                     /locus_tag="PLS35_20010"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNI21013.1"
                     /translation="MRRRAWIALAAVMLLMCLHPSMSVAVERAEAEETARLLAKLLES
                     GRAVIERNQSLIDDPHKGEKGFTPELFEQQLMREFHAKTGIDLNALPAAPVSMLIPPL
                     AKELLPALVQASREVIRDAQVVINQRGIGYKNFIPATYGSQASARFSKAAHVRLKQTT
                     IQPRNPKNEPDEYEASVLKWLSARPMAEAYVSELTDGGRTLRVVMPIYYAKDCLACHG
                     EPKGDLDISGYPKEG"
BASE COUNT          497 a          730 c          798 g          518 t
ORIGIN      
        1 tctggcggcg gtgcaccgct ccgccctcaa gggcgttgat tgtcgggcgg aggtcatgaa
       61 gtcatgcatt tccgccgggc gttctccgac tgatcgatga ccagcacgtg ccatgggctg
      121 gttgatcgag gagaccggac cacgctcacg cgaaggtctg ccatgcggac aacggtctta
      181 aaggggcaac gagggatcgc ctgggcctgc tggctggttg tactggccac agtctggctg
      241 ttgcctgtgg cgccggtggt cgctcaggaa gcggtccttg aatttcccat tcggcctttt
      301 aatgtcgatg tgatgctccg ccagcagacg tttgcgccag acgatcgtga gatcggcgtg
      361 cagctcggca ttacggcggt acgctacggc aatgtcgagg ttcggaccat ctatcaattc
      421 ttcagcatcc atacgcagga cttcagcacc gaccagcatt cgctgttcgt gaatcctcgc
      481 tggaataact tcatcgacat cctggatttt cccaagcaca aaccgatcag ccgcatcatc
      541 cgccatgtgc tgtttggacc gttggaggat cgggccgttc cctatgtggg agtattggcc
      601 ggaggtgtct tgcccggccc tggtaaccgt gctccaggcc atttgatcgg cggccaggtg
      661 ggagtgcggt tcccggtcgc acagagcctc tcagtggata tcagcgtgca atacagtcaa
      721 tacggggtgg attttcgcgg agacgcggga caggcgcagc aatgggtatt cctgacaggg
      781 atacggtttt aatcaaggca acctggtgcg gccgaatctg ggcagcacta tgctgtctgc
      841 tgttgtccgg ctgcggcttg ctcatcgatg cggttgaaca ggtctggccg gtcgccggca
      901 gtaaagtcga tatcatctgc gaacgcgggc gtgttcaggt cggcctggcg atggagccgt
      961 tccgtccctt cgtgtttccg acgatctgga cggacgaagg ggctcgtgtc acggggctcg
     1021 atgtcgaact cacccgggcc atgacggacg aactgtcccg acggtgcgga catccggtga
     1081 ctcccgtcct gcatctcgtg cgcttcaggg atctgttccg gctcctgaat gaaggacact
     1141 tggattggtt cgtgtcggct gtcgcgacaa acacgcctgc cccgtcccgc gccggagtcg
     1201 cctattccct gccctatttt tacgggggag gtatcagcgg gatcacaacg agcacgagcg
     1261 tcctggagcg ggtgcgggag aatcttcagc aacaggcgac gcatcctgca gccgatcggc
     1321 tcgcggccac gagtcatgcg ctggatggcc tcaccatcgc ggtgcaggat gaaaccagcg
     1381 cgtactacta tgccgaagcc aactggggcg ccaaccggct gatcgtgtgc gattcgttac
     1441 cggcagcctt cgaatatgcc gacgccaaga ccgagccgcg tgtcgatatt attctgggcg
     1501 cacaaccggt gttggaatat atggtcaagc gggttcgcaa ggactggtca ctcttgaccc
     1561 gtgagaacgg ccgccccctg ttcctgacca aggccgacta cggagtggtt gtggctgagg
     1621 agagttaccg cctccgctgg ctggtgaacg atctactgtt gaaactggac gaatccggtc
     1681 gcctgggtga gatgcggaca cgttggctcg acgacgagta tgcgtttccc cggagagcct
     1741 cgttggaagg attacccttc gatgtggaga agatggcgtc ccactacatc cagggcacct
     1801 gccgcatcag gcccggatca tgaccgcctg gagaggagca caatcatgag acgtcgtgca
     1861 tggatcgccc tggctgcagt gatgctgctg atgtgcctgc atccttcgat gagcgtagcg
     1921 gtggagcgag ccgaggcgga ggaaaccgcg cgcctgctgg ccaaactgtt ggaatcaggg
     1981 cgggccgtca tcgaacgcaa ccagtcgttg atcgacgatc ctcacaaggg cgaaaaggga
     2041 ttcacaccgg agttattcga gcagcaattg atgcgggagt ttcacgccaa gaccgggatc
     2101 gacctgaacg cgctgccggc cgcgccggtg tccatgctca ttccgccact cgcaaaggag
     2161 ctgctgccag ccttggtcca ggccagccgc gaggtcatcc gtgatgccca ggtcgtcatc
     2221 aatcagcgcg gcatcggcta caagaacttt attccggcca catacggcag tcaggcctcg
     2281 gcacggttct ccaaggccgc gcatgtgcgg ctgaagcaga ccacgattca accacgaaac
     2341 ccgaaaaacg agcccgacga gtacgaagcg tcggtgctga aatggctctc cgccaggccc
     2401 atggccgagg cctatgtgag cgaattgacc gacggagggc gtaccctacg ggtagtcatg
     2461 ccgatttatt acgccaagga ttgtctggcc tgccatggag aaccgaaagg tgatctcgac
     2521 atttccgggt atcccaaaga agg
//
LOCUS       DAOQEW010000360         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_22304_length_2523_cov_6.964749, whole genome shotgun sequence.
ACCESSION   DAOQEW010000360 DAOQEW010000000
VERSION     DAOQEW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22304_length_2523_cov_6.964749"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLS35_20015"
     CDS             <1..574
                     /locus_tag="PLS35_20015"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNI21014.1"
                     /translation="ERSPEMGVKWHGAGRRAVVVGALVAATLGLFSTSPAAEFKMGVV
                     DPQVILEKSKAGKRALDGLREYVATRQKLLSRDEEELRNTEKQLKEQASKLTDAEKKE
                     KETSFRSKIQDYQKRAQEFNQELQGKQKELVDEYMKKIAVATQAVADKGGYQLVLDKG
                     SEQTVKIVIFNKDTIDLTEQVIKEFDRTNK"
     gene            complement(578..1648)
                     /locus_tag="PLS35_20020"
     CDS             complement(578..1648)
                     /locus_tag="PLS35_20020"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNI21015.1"
                     /translation="MPFSRREFFNNAGFGLLLLPALLRSPQTILRHLLFDPEGPLVLP
                     KPIPVNPFMRGGRALVCIVYGTDPARMLGRALELLGGIDRMGVRGTRVLLKPNVLNDQ
                     PPPSTTNPLVVKAMANIVRANGAAEVVVADGSGIIRLPTSANLTSTGMRTAAESAGAR
                     VLALEDEPWVRLEPPNAKAIGHFYFSKPVYDADVVINMPVIKTHRFAEFSCSLKNFVG
                     AVHPRYRPSVTFWSGDWHERIAEINLAVHPVLTVADGTTTMIDGGPTSGTPSRTDLLI
                     CSGDRVAVDLTALALLRSFGKSSKLQGRRIVDQRQIRRAADLGLGVGSGRGIELVTEA
                     VDPAPPAFLRLVEHIRQELLES"
     gene            complement(1652..2032)
                     /locus_tag="PLS35_20025"
     CDS             complement(1652..2032)
                     /locus_tag="PLS35_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNI21016.1"
                     /translation="MKHNPGFLKVVEEARARVRECTVGDVKAKLDRGERFHFVDVRED
                     DEFSQDHAAGATHIGKGVIERDIETAIPDRQQPIVLYCGGGYRSVLAADSLRQMGYTN
                     VISMDGGIKAWRAAGYPLEKGPRS"
     gene            complement(2029..>2523)
                     /locus_tag="PLS35_20030"
     CDS             complement(2029..>2523)
                     /locus_tag="PLS35_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI21017.1"
                     /translation="DDETARLVEPQAPGISARFEAMRVLSDAGIPTGISVAPIIPGLN
                     EEAIPEILSRARQAGATSATWSLLRLPGHVEQVFIERMREAFPDRVSKIMHRIREVRG
                     GALSDAEFFSRHRGMGTYWRCVEQLFELGRRRAGFEPDDVPIPQTFRRPTAQQSLFCV
                     EESL"
BASE COUNT          601 a          762 c          703 g          457 t
ORIGIN      
        1 cgaaaggagt cccgagatgg gtgtgaagtg gcacggagca ggccggcgcg cggtcgtggt
       61 cggggcgctt gtcgcagcga ccctcggctt gttttcaacg agtccggcgg cagagttcaa
      121 gatgggggtc gtcgatcctc aggtgattct cgagaagagt aaggccggca agcgagcgct
      181 ggacgggctg cgggagtatg tggcaacacg ccagaaactg ttgtcccggg atgaggaaga
      241 gttgcgcaat acggaaaagc agctcaaaga gcaggcctcc aagctgaccg acgccgaaaa
      301 gaaagaaaaa gaaacgtcgt tccgcagcaa gatccaggat tatcaaaagc gcgcgcagga
      361 gtttaaccag gaactgcagg gcaagcagaa ggaactcgtc gacgagtaca tgaaaaaaat
      421 cgccgtagcc acccaggccg ttgccgacaa gggcggctat caattggtgt tggataaggg
      481 cagcgagcaa acggtgaaaa ttgtgatctt caataaagac acgatcgatt tgaccgaaca
      541 agtcatcaag gaattcgacc gcaccaacaa gtaacggtca ggactccagt aattcctgcc
      601 gaatgtgctc caccagccga agaaacgctg gtggagccgg gtccaccgcc tccgtaacaa
      661 gttcgatccc gcgtcctgat ccgactccta agcccaaatc cgccgcgcgt cggatctgcc
      721 gttgatcaac gatccgcctg ccctgcagtt tcgacgactt gccgaacgac cgtagcagcg
      781 caagcgccgt cagatccacc gcaactcggt ctccactgca gatcagaaga tccgtcctgg
      841 acggggtacc tgatgtcgga ccgccatcga tcatggtcgt ggtcccatcg gccacggtca
      901 gtacgggatg taccgcgaga ttgatctcag caatgcgttc atgccaatcg ccgctccaga
      961 atgtgaccga gggacggtac cgtggatgga ccgcgcccac gaaatttttg agactacagg
     1021 agaactcggc aaagcgatgc gtcttgatga ccggcatatt aatcacgaca tcagcgtcat
     1081 acaccggttt cgagaaatag aaatggccaa tggccttggc attgggcggt tcgagccgca
     1141 cccaaggctc atcttcaagc gccagcaccc tcgcgccggc tgattccgct gccgtccgca
     1201 taccggtgct ggttaaattg gcagaggtgg gcaggcggat gattccggac ccgtcggcca
     1261 ccaccacttc tgccgctcca ttcgctctga ctatattagc catagccttc accaccagcg
     1321 gattggtcgt cgagggcggc ggctgatcgt tcagcacatt aggtttgagc aggacacggg
     1381 tgccgcgaac ccccatccga tcgattccac caagaagttc aagcgcacgc ccaagcattc
     1441 gcgccggatc tgtgccgtac acaatgcaga cgagggcgcg ccctccccgc atgaatggat
     1501 tgacaggaat gggcttcggc aacaccaacg gcccctcggg atcgaagagc agatggcgca
     1561 ggatagtctg tggagaccgt agcagcgccg gaagcaggag gagaccgaat ccggcattgt
     1621 tgaagaattc tcgtcgtgaa aacggcatgc gttaggagcg cggccctttc tccaggggat
     1681 aaccggcggc gcgccaggcc ttgatgccac cgtccattga gatgacattc gtatacccca
     1741 tttgccggag actgtcggcg gctaataccg accggtaacc acccccacaa tacaacacga
     1801 tcggctgctg cctatccgga atcgccgttt caatatcccg ttcgatcaca cccttgccga
     1861 tatgagtggc ccctgccgca tgatcctgag aaaattcgtc atcctcgcgg acatccacaa
     1921 aatggaagcg ttcaccgcgg tccagtttgg ccttcacgtc accgacggta cattcgcgca
     1981 cacgggcccg cgcctcttcc acgaccttta agaatccggg attatgcttc acagtgactc
     2041 ctccacacaa aataacgact gctgcgccgt cgggcgtcga aacgtctgcg gaataggcac
     2101 gtcatccggc tcgaagccgg cgcgcctgcg acccaactca aacaactgct cgacacagcg
     2161 ccaataggtc cccatgccac gatgccggct gaaaaattcc gcgtcgctca aggcgccccc
     2221 gcgaacctcc cgaatgcgat gcataatttt cgaaacccga tcaggaaagg cttcgcgcat
     2281 acgctcaata aagacctgct cgacatgtcc cggtaaccgc aacagactcc aggttgccga
     2341 ggtcgctccc gcttgcctcg cacgagacaa tatttcagga atggcttctt cgttcaaccc
     2401 gggaatgatc ggcgccacag agattccggt cggaatgccc gcatcggaaa gcacacgcat
     2461 cgcctcaaag cgcgcactga tgccgggagc ctgcggttcc accagtcgcg cggtttcatc
     2521 atc
//
LOCUS       DAOQEW010000361         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_22437_length_2515_cov_8.728862, whole genome shotgun sequence.
ACCESSION   DAOQEW010000361 DAOQEW010000000
VERSION     DAOQEW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22437_length_2515_cov_8.728862"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLS35_20035"
     CDS             complement(<1..287)
                     /locus_tag="PLS35_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21018.1"
                     /translation="MTRITSWHLVCAVALGLSGTPVWADSLCAEKALQAAADAQLKKA
                     EELERAGQPREAFAAATKANGDCVSDTQRHEGVQQRTAKAIGAEEEKKARL"
     gene            complement(298..924)
                     /locus_tag="PLS35_20040"
     CDS             complement(298..924)
                     /locus_tag="PLS35_20040"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HNI21019.1"
                     /translation="MLTRFLLCLVLAAATLPTAGLALAAERTNVEYSADQIIESEDGA
                     TKSRIYSTPTKERRDMSQGGAAMTMITRHDKHVSWTVMPEEKMYMEAPATKQGQDKSD
                     LSGYQIEQTALGEETVNGVVMNKSKIIMTGNDGTKMGGFMWTTKEGILAKIDALAVEK
                     GQKARFKMEQTNIQIGKQPAELFEIPKGFEKLDMGGMGMDMLKGMMGR"
     gene            complement(1132..2307)
                     /locus_tag="PLS35_20045"
     CDS             complement(1132..2307)
                     /locus_tag="PLS35_20045"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNI21020.1"
                     /translation="MTRSATPVMSLSPNLDFLRAIAVLLVYFFHLFITTGPRLPDFLA
                     QFGVILFFVHTSLVLMFSLQRIDLKGQRLFATFYLRRLFRIYPLSIVCVSVIILLQLP
                     RAPWWPWTQPDTSTILANLLLYTEFTYKPVVTSVLWSLPYEVAMYAVLPFCYLAGKAY
                     GIRGILALWGLAVVGGFVQPYISWRLGLAQFAPCFIAGVACYFMGYGARRRPLPFWGW
                     PLILLAAAGIQGFGSYYEYEQVSRWVLCLMIGLTAPFFAELNRPMLNKATELIARYSY
                     GIYLTHLHAQWAALVLLKDQPAAVRYSVLVVLSVGLPVALYHMVEAPMIRLGARLAAN
                     LPTKSVPVVRPASPQRALPAPAEAAPYGRPSQPVRPRPGQAPAVRTGKSAPAPASAR"
BASE COUNT          524 a          700 c          778 g          513 t
ORIGIN      
        1 tccaggcgtg ccttcttttc ctcttctgcg ccaatggcct tggcggtgcg ttgctgcacc
       61 ccttcgtggc gttgagtatc gctgacacag tcgccgttcg ctttggtcgc ggcggcaaag
      121 gcctcgcgtg gttggccggc ccgttcaagc tcttccgcct tcttcaactg cgcgtctgcc
      181 gctgcttgta gtgccttctc cgcacacagg gagtctgccc agacgggagt tcccgacagg
      241 ccaagcgcga cagcacacac gagatgccaa gatgtgattc gtgtcacgac tgcctcctta
      301 gcgtcccatc atccctttca gcatatccat gcccatgccg cccatatcca gcttttcgaa
      361 gcccttggga atttcaaaaa gttcggccgg ttgtttgccg atctgaatat tggtctgctc
      421 catcttgaag cgcgctttct gtcccttctc gacggccagg gcgtcaatct tggccagaat
      481 gccttctttc gtggtccaca tgaaaccgcc catcttcgtc ccgtcgttgc cggtcatgat
      541 gatcttgctt ttattcatga cgacaccgtt gacggtctcc tctcccaatg ccgtctgctc
      601 aatctggtag ccggaaagat cggatttatc ctgcccctgc ttagtggccg gcgcttccat
      661 gtacattttt tcttccggca tgaccgtcca gctgacatgt ttgtcatggc gggtgatcat
      721 cgtcatcgcg gcgccgcctt gagacatgtc gcggcgttct ttggtcggtg tcgaatagat
      781 acggcttttg gtggctccat cttcagactc gatgatttga tcggccgagt attcaacgtt
      841 cgtgcgttcg gcggcgagtg caagtcccgc ggtgggtagc gtggctgcag ccaggacaag
      901 acacaacagg aatctggtca acatgaaaac ctctcgttgg atggtgaatg gatggcgaat
      961 gtgtcgcatg ccgcatcggg gaacgtctgg acccgtgcgg gccatgggcg cgaagggtac
     1021 gcctggtgcg cgagcctgtc aattgatgcc gcgtgcaccc cgaaggtgaa gggaggcaca
     1081 ggggtgtggt tctatcacgg aggaactgaa gacgaaggga cgcggagggt attacctcgc
     1141 ggaggccgga gccggagcac ttttacccgt gcggacagcc ggcgcttgcc ctggcctggg
     1201 ccgtacgggc tgggagggcc tgccgtacgg tgcagcttct gccggtgcag ggagcgcccg
     1261 ttgcggcgaa gccggtctga cgacgggaac ggatttcgtt ggcaaattcg ctgccaggcg
     1321 agcgcccagc cggatcattg gtgcttccac catatgatag agcgctacgg gcaggccgac
     1381 gctgagcacg accaggacgc tatatcgaac ggcggccggt tgatctttga ggaggaccag
     1441 tgccgcccat tgcgcatgga gatgggtcag atagattcca tatgaatagc gagcgatcaa
     1501 ttccgtggct ttgttcaaca tcggccggtt caattcggca aagaacggcg cggtgagacc
     1561 gatcatgagg cacaggaccc atcgcgagac ctgctcatat tcgtagtaac tcccaaatcc
     1621 ttgaatgccg gcggcggcaa gaaggatcag tggccagccc caaaatggca gcgggcgccg
     1681 gcgagctcca taccccataa agtagcaagc cactccggca atgaagcagg gggcaaattg
     1741 cgccaggccg agacgccagg agatgtatgg ctggacgaag ccgcccacca cggcgagacc
     1801 ccaaagagca agaattcctc tgatcccata ggccttcccg gccaggtaac agaatgggag
     1861 aacggcgtac atggcgactt cataaggaag actccagagc accgaggtca cgacaggttt
     1921 ataggtgaac tcggtataca gcagaagatt cgccagaatg gtcgaggtgt ccggttgtgt
     1981 ccatggccac cagggagccc ggggaagctg aagcaagatg atgacactga cgcacacgat
     2041 gctgagcgga tagatccgaa acaggcggcg tagatagaac gtcgcaaaca gtctctgccc
     2101 cttgaggtcg atccgctgca gggagaacat caacaccagg ctggtatgaa caaaaaagag
     2161 aatgacgccg aattgcgcga ggaagtccgg caagcgcgga ccagtcgtga tgaacaagtg
     2221 gaagaagtac acgaggagaa ctgcgatcgc gcgcagaaag tccagattcg gggagagcga
     2281 cattaccgga gtggcactgc gggtcatctc gcgagggtcc ttctcgctga atgaatggtg
     2341 aatatgccct gtgctctgac cggccaggtg gtcccgcacg tcacggacga agccgagccg
     2401 cgcgcatctg tcgagatgcg tgggagccgg gaggcctgcg atgcgttgaa gtgactgtca
     2461 ggtcgccaat gtcccggcga ggagtttcgg gaattgggcc acgctgagag ccctg
//
LOCUS       DAOQEW010000362         2501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_22682_length_2501_cov_8.947261, whole genome shotgun sequence.
ACCESSION   DAOQEW010000362 DAOQEW010000000
VERSION     DAOQEW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22682_length_2501_cov_8.947261"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLS35_20050"
     CDS             complement(<1..1204)
                     /locus_tag="PLS35_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNI21021.1"
                     /translation="MRLSLRFILPLLLVLGVVAYSVVPLVDSLTLKWFTRDLESRSKL
                     LASTMEVPLADLVVSRSRTKIFAYFHKVIQDERLYALGFCDTQQRLLYQTLTYPDQLS
                     CESLAHLAPGSSEVVDFAQGPLHVVVATIQSGGKVQGRLMLVHDMSFVHQRSTDTKWY
                     IFYLFVGLAGVISAVTVLVAQLSWRGWVAGVRAMLTSKGLPAESSQAHSAELHPVAQD
                     LHALVRELEADRRMRDESQITWSPASLKTILHEHLSGDEILIVSNRQPYIHNRRGQKV
                     EVQVPASGLVSALEPVMRACSGVWIAHGNGSADKEVVDNRDHVRVPPDHPSYDIRRIW
                     LSPDEESGFYYGFSNEGLWPLCHNAHVRPTFRTSDWEQYVAVNERFAKAVVEEAKTDD
                     PVILVQDYH"
     gene            1243..>2501
                     /locus_tag="PLS35_20055"
     CDS             1243..>2501
                     /locus_tag="PLS35_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNI21022.1"
                     /translation="MVWGMYPYGLIGNCHVSAHIHESGSVDWLCLPRPDSDPVFGRIL
                     DPEGGHFSISSPTSSAQVKTTQRYLPNTNILVTEVSTSNGDTFRITDFCPRFEQYGRI
                     YRPAALFRIVEPLAGTPSIRVSCRPVTGWGKDYARGMRGNSHVRYDIRGEYLRLLTNM
                     PLTYLYEERPFALTEKTYFALTWGLGIEDDLAKVSHEFLDQTTRYWRMWVKHCSIPVL
                     HQEEVIRSALALKLHCYEDTGAILAALTTSLPEEPGGPRNWDYRYCWLRDAYFSLTAF
                     HNLGHFEEMEGFLKFLLNIAYTHEHSRERLAPVYTLSQDLPLPETEHPNWAGFSGSAP
                     VRNHNQAAEHIQNDVYGELVLALTPIFTDNRFYDLRTKDQEQLVANLARLCDRTIAQP
                     DAGLWEIRNGWQEHSFSNLMCWAGLDRA"
BASE COUNT          548 a          785 c          680 g          488 t
ORIGIN      
        1 agtgatagtc ctgcaccagg atgacgggat catcggtctt ggcctcttcg accacggcct
       61 ttgcgaaccg ttcgttgacc gcgacatatt gctcccagtc agaagtcctg aacgtaggcc
      121 gcacatgcgc attatggcac agcggccaaa gcccctcgtt ggagaaacca tagtaaaagc
      181 cggactcttc atccggcgac agccaaatcc gacgaatatc gtacgaggga tgatccggcg
      241 gcacgcgaac atgatcccga ttatccacca cttctttgtc ggccgatcca ttgccgtgag
      301 caatccatac gcccgagcag gctcgcatca ccggctcgag ggctgagacc agtccactgg
      361 ccggcacctg gacttcgact ttctgtccgc gccggttatg gatgtacggc tgtcgattcg
      421 acacgatgag aatttcatca cccgacagat gctcatggag aatcgtcttg aggctggccg
      481 ggctccaggt aatctgactt tcatcccgca tacggcgatc ggcttccagt tccctcacca
      541 acgcatggag atcctgcgca accgggtgca actccgcgga atgggcctgg ctggattccg
      601 ccggcagccc tttgctggtc aacatcgccc gcaccccggc cacccatccg cgccaactca
      661 gttgcgccac cagcacggtg acggccgaaa tcacccccgc gaggccgacg aacaaataaa
      721 agatgtacca cttggtgtcg gtgctgcgct ggtgcacaaa actcatgtca tggaccagca
      781 tcaaccggcc ctgcaccttg ccgcccgatt gaatcgtcgc gacgaccacg tgcagcggcc
      841 cttgggcaaa gtccacgact tcagaagaac cgggcgccag gtgcgctaag ctttcgcagg
      901 acaattgatc cgggtaggtc agggtctggt agagcagacg ctgctgggta tcgcagaagc
      961 ccagcgcata caaccgttca tcctgaatca ccttatggaa gtaggcgaaa atcttcgtcc
     1021 gggagcggga gacgacgagg tccgccagtg gcacttccat ggtgctggcc aacaacttcg
     1081 accggctttc caggtcacgc gtgaaccact tcaatgtaag ggaatcaact aagggcacca
     1141 cgctgtacgc gacgacacca agcaccagca aaaggggaag gataaaccgg agggacaatc
     1201 gcattttttc tcccgcagtt tactttgaga gcaaaatctc ttatggtctg gggcatgtac
     1261 ccctacggac tcatcggcaa ctgccacgtc tccgcgcaca tccatgaaag cgggtcagtc
     1321 gactggctct gcctgcccag accggacagt gatccggtct tcggacgaat ccttgatccg
     1381 gaaggagggc atttttccat atcaagccct accagctccg cacaagtcaa gacaacgcag
     1441 cgctacctgc ccaatacaaa tatcctcgtg accgaggtct ccacgtccaa cggggacacc
     1501 ttccggatca cggatttctg ccctcgattc gaacaatacg gccgcatcta tcgtcccgcg
     1561 gcgttgtttc gcatcgtcga accgctggca ggcacgccat ccatccgcgt cagctgccgg
     1621 ccggttacgg ggtgggggaa agactatgcg cgcggcatgc gcggcaacag ccatgtgcga
     1681 tacgacatcc gcggggaata cctccggctg ttgaccaaca tgcccctcac gtatctctac
     1741 gaagagcgtc cattcgccct gaccgaaaaa acctacttcg cgctgacctg gggcctgggc
     1801 atcgaggacg acctcgccaa agtcagccat gaatttctcg atcagaccac gcggtactgg
     1861 cggatgtggg tgaaacattg ttcgattccc gtgttgcatc aggaagaagt gatccgctcc
     1921 gccctggcgc tcaaactgca ctgttatgag gataccggtg ccatcctggc cgccttgacc
     1981 accagcctcc ccgaggagcc gggtggcccg cgcaactggg attaccgtta ctgctggttg
     2041 cgcgatgcct atttctcgct caccgcgttt cacaatctcg ggcacttcga ggagatggaa
     2101 gggtttctca aatttctcct caacatcgcc tacacccatg aacattcccg cgaacggctc
     2161 gcgccggtgt acacgctgag tcaggatctc ccgctgccgg aaactgaaca tcccaattgg
     2221 gccggcttct ccgggagcgc cccggtgcgt aatcacaatc aagccgccga gcacattcaa
     2281 aacgacgtgt acggcgagct ggtgctggcg ctgacgccca tttttaccga caaccgcttc
     2341 tacgacttgc ggacgaaaga tcaggaacag ctggtggcca atctggcgcg actctgcgac
     2401 cgcacgattg cgcaaccgga cgcagggctg tgggaaattc ggaacggctg gcaggaacat
     2461 tcgttttcga atctgatgtg ttgggccggc ctcgaccggg c
//
LOCUS       DAOQEW010000363         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_22879_length_2490_cov_6.985626, whole genome shotgun sequence.
ACCESSION   DAOQEW010000363 DAOQEW010000000
VERSION     DAOQEW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22879_length_2490_cov_6.985626"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1557)
                     /locus_tag="PLS35_20060"
                     /pseudo
     CDS             complement(286..1557)
                     /locus_tag="PLS35_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1884..>2490)
                     /locus_tag="PLS35_20065"
     CDS             complement(1884..>2490)
                     /locus_tag="PLS35_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HNI21023.1"
                     /translation="IMEKRMFLRLLAASLFMTGCVTIPTGPTVLVLPGQQKSFEHFRD
                     DDIACQQYAWALTAPVSQAAADAAVSPAVVATAIGAAAGAIIGSATGRAGQGAAIGAG
                     TGLVFGSSASGHTAYLTSYELQRRYNMAYVQCMYSRGNQVPGSAVYRQAPANYLPPGA
                     PPPGYPPPGTPAPDLPPQPKPPPNYPPPNTLPPEGVPPQRK"
BASE COUNT          467 a          803 c          687 g          533 t
ORIGIN      
        1 gctaaggcta tgaccaaaac cttgacctgt ttggttccgg cttcgccggc ttaggtcata
       61 gccttataca caactcatct gacatcataa atatcgttat gaaacaataa gttaccgaat
      121 tgcataaaaa cctgctcgtt tgagcatgtt tcatctaaaa atcaacgatt tagatcgctt
      181 ttttgctcat gctgaatgag ttgtgtataa ggctatgggc ttaggtggcg caagaaagtc
      241 agggtcgcgt tagcgaccct gtttagccgc tttgagcggc tcacatattg aaagcccccg
      301 gctctgccgg gggaaggtta ctccacgcac cggcaacagc accagtccga gattcctggc
      361 ctgggcattg acacattcgg ccgtggctgc acgtgcctta tcaagcgcct tggcctcgtc
      421 tgtgcccatc cgttcccgcc agtctcccac ggccgtactg tctccgggtt tcctctggtg
      481 cggatcgacc gccactttcg gcgtcaccga aacatcgccc gcagcctcgt gtcccgccgt
      541 ttcgtccgtc gttgtcttct tggctttcgg cttgggcacg ggagccatca ctcgcgttgg
      601 tgtggtcacg gaatccatct ggtcgtgcgc cggaaatcca ccatccatca accagtcgtc
      661 gggtgcccgt ccgcaacgat ccacgacctg ctccaccatg ggcaccatct gccccatgtc
      721 actgcccgtg gtgacgacat caacgcctac gatcacctga cttgccgtgt cgcggccaaa
      781 ttcgatgtgg tacaccggcc ggaatccgcc atcccccatc ttcatgaccg tcgcctcggc
      841 gtcagtcgtc gagcgacggg cgtcctcggg tttctggcct ggcttctttt tgatggccgc
      901 cagttcaggg aggcgggcca gagcagcttc aagatacgct tcacgttctt ctgccgcacg
      961 ccgctgcgcc gcttgcttac gagggctttc gcccacagga tcgtcgacgt aggccttgag
     1021 ttgggcaact cttttccgag ccgtttcaag atgatccttg agtttgtctt ctcggcgaaa
     1081 ggaagcggca ccggcactgg ctcgtactcg catgccgtcc tgtgccactg ccttcagttt
     1141 gacggcgcct gccgccatca ggctggagat gctgtccgtc agcagttcgt ccatcgcctc
     1201 gacctcgtgg cgaaaatcag ccagggtatg gtaattcacg tccacgcccc cacagatccc
     1261 ccggtaggcg tcgtcttccc cagtcaactg ctcaagcctt cgggcactgc ccacaccttc
     1321 ccatgtcgca aaaagccaca gggcgaacag aatttcaggg gcaatcgccg gtcgccccgg
     1381 cccgccttca acggctttga tcccagcata cagccgtgac atgttctgcc gctccacata
     1441 accccagacc atccgtgccc agtgccccgg cagcaacagg gattccagac tgctcgcgcg
     1501 cggcaccact tggttacgat caggcatcag aacaagcgca gcagccttca gcaccaagtc
     1561 actgccagaa ttcgggcgtt tgcgtttgcg ggtgtcgttc catcagagaa aatgaaggag
     1621 gaagtctcag tcattgccgt cacctcgtaa tcaacatgat cacttcccca ctgtactccc
     1681 gccacaggct tcagcccgaa tcttgcagga tattttcaca ggttctcact ccctggcaag
     1741 tcaagtcgac ggatcgacca ggaccgctac catggtcagg tcgttcacca cctcatcaag
     1801 cacgtgccca ctttgggcct ccaacaagat cggtgacaag ggcatcactc ccctcggtct
     1861 tgctgcgcgc atgttggcca ggcctatttg cgttgggggg gaacaccctc cggcggcagc
     1921 gtattcggcg gcggatagtt cggcgggggt ttgggctgcg gtggcaggtc cggtgcaggc
     1981 gtgccgggcg gcggatagcc cggtggaggc gctccgggtg gcaggtaatt cgccggtgcc
     2041 tggcggtaca cggccgaccc cggcacttgg ttcccacgcg agtacatgca ctgaacatag
     2101 gccatgttgt agcgccgctg cagctcgtag gaagtcagat aggcagtgtg gccactcgcc
     2161 gagctgccaa aaaccagtcc ggtccccgca ccgatcgcgg caccctggcc cgctcggccg
     2221 gtcgctgaac cgatgatcgc acccgcggca gcgccaatgg cggtcgccac cacggcaggg
     2281 ctgacggccg catcggcagc cgcctggctg acgggcgccg tcagcgccca tgcgtattgc
     2341 tggcaagcga tgtcatcgtc ccggaaatgc tcgaaggatt tttgttgccc cggcaggacc
     2401 agcaccgtgg gaccggtcgg tatggtcacg caccctgtca tgaacaagct ggccgcaagc
     2461 aaccgcaaaa acattcgttt ttccatgatg
//
LOCUS       DAOQEW010000364         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_23362_length_2462_cov_6.018280, whole genome shotgun sequence.
ACCESSION   DAOQEW010000364 DAOQEW010000000
VERSION     DAOQEW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23362_length_2462_cov_6.018280"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..417)
                     /locus_tag="PLS35_20070"
     CDS             complement(124..417)
                     /locus_tag="PLS35_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21024.1"
                     /translation="MKITIQRTGGFAGLQEALASVDTDQMPAAEARRVEELVAKAAFF
                     DLPAIVGGNIKGADVYRLRVTVTDKSRHQSVEFPDHDSPELTSLRALVRSLAP"
     gene            complement(492..1967)
                     /locus_tag="PLS35_20075"
     CDS             complement(492..1967)
                     /locus_tag="PLS35_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21025.1"
                     /translation="MLQEIIESPTATMRPATEHMSSPSASMTVRVGFVPTPTREYRQH
                     RKLLRATTGIASRASVRMMPDRESARVVIPIPIPIPRIIGSRVLMWKQDPSVSEIGIR
                     KAFLPGLLLAGPRDSRISIQGMPLVSPNAMADYIETPGTQEFDAVHTFAVVRQMLTLA
                     QRARGGSVAPWQWNSASNTEGMSVFPRAGVTPNAFYSRNLKALKFFFFTKPGSPPGSP
                     QVFTCRSFDIVAHETGHAVLDGLKPGWLDASNHPQTGGLHESFGDLCAIFLTLSQLDQ
                     VEAIIAQTKANLHDKTFLADMAEEFGLALGRPNGLRNADNDLKLSEVTNEVHDLSQVF
                     TGAIYDVLADIFAFERKPGMRDDARVLLEVAEYLFGLILRAINAAPAANATFADMANQ
                     MLTIAHAEGKPVQYRNFIRNRFTVREIVVSPTPLTADHKAGMKLMPAFTEEIRMSQDR
                     RGCCGTMQLPEYNEGDRVFEDDLEELRNLFSDNGRAKVLHR"
BASE COUNT          499 a          666 c          762 g          535 t
ORIGIN      
        1 aaaaagatcc cgccaagaaa aatgccatca tgaacgctct ccacgccagc ggtcagaccg
       61 gggattagtg aaatcgaaga gcttgtagga agcccggccg tcccacacac gacccacggt
      121 cgactaggga gccagggacc ggacgagagc tctcagggag gtcagttcag gggagtcgtg
      181 atcaggaaat tcgacgctct ggtgacgcga tttatctgta acagttactc gcagacggta
      241 gacgtctgcg cctttgatgt tgccgccgac tatggccggt agatcaaaga acgcagcttt
      301 cgcgaccaat tcttcgacgc gcctcgcctc cgcagccggc atctgatccg tatcgacaga
      361 agccagtgcc tcttggagcc cggcgaaccc accggtgcgt tgaatcgtga tcttcatcgc
      421 ctgtcctcag tggagctcaa tggcggcccg ggcaccgtat ggcctcccga gccgcctcat
      481 tgagactgat cttatcgatg gaggaccttc gctcttccat tgtcgctgaa aaggttccgg
      541 agttcctcca gatcgtcttc gaatacacgg tccccctcat tatattcggg gagctgcatg
      601 gttccgcagc agcctcgccg gtcttggctc atccggattt cttccgtaaa cgccggcatg
      661 agtttcatac ccgctttatg gtcggcggtg agcggcgtcg gcgagacgac gatttcccga
      721 accgtgaagc ggttgcgaat gaagtttctg tactggaccg gcttgccctc tgcgtgcgca
      781 atcgtcagca tctggttggc catatcggcg aaggtggcat ttgcggccgg agcggcgtta
      841 atggctcgga ggatcaggcc aaacagatac tccgcgactt cgagcaggac cctggcgtcg
      901 tcccgcatcc cgggttttcg ctcaaacgcg aagatatctg cgagcacatc gtagatggct
      961 cccgtgaaga cctgtgacag atcgtgcact tcgttggtca cctcgctcag cttcaaatca
     1021 ttgtccgcat tgcggagacc gttcgggcgg ccgagtgcaa ggccgaattc ctcggccatg
     1081 tcggcaagga atgtcttgtc atgcaagttc gctttggtct gggcgatgat cgcctccacc
     1141 tggtcgagtt gggaaagcgt cagaaagatc gcgcagaggt cgccgaacga ttcatgcaat
     1201 ccaccggtct gcggatgatt gctggcgtcg agccaccctg gcttaaggcc atccaggacg
     1261 gcgtggccgg tttcatgcgc cacgatatcg aacgagcggc aggtgaacac ctggggagac
     1321 cccggggggc tgcccggctt ggtgaagaag aagaatttca aggctttgag attgcgactg
     1381 tagaacgcat tgggagtgac gccggcccgc ggaaagacgc tcatcccttc cgtgttggag
     1441 gcgctgttcc attgccacgg tgccacggat ccgccacgtg cccgttgcgc gagagtgagc
     1501 atttgccgga cgacagcaaa ggtgtggacc gcatcaaact cctgcgtccc tggagtctca
     1561 atatagtccg ccatggcgtt gggggagacg aggggcatgc cttgaatgga aatgcggctg
     1621 tctctcgggc ccgctaataa cagaccgggg agaaaggcct tgcgaatccc gatttcgctg
     1681 accgaggggt cctgcttcca catcagcact cgagagccga tgatccgggg gatgggaatc
     1741 gggatcggga tgaccacccg ggctgattcc cggtcgggca tcatgcgaac ggaggcacgg
     1801 ctggcgatgc cggtggtagc acgcaagagt tttcgatgct gccgatattc acgtgtcgga
     1861 gtcggaacga acccgacgcg aaccgtcatg ctggccgaag ggctactcat gtgttcggtg
     1921 gctggtctca tggtcgccgt cggactttca atgatctcct gaagcatggc tcttaccctt
     1981 gtcctttctg agtcgatagc ggccgcgctg gattttggat tggaggcacc gactcgatgt
     2041 gagggagaat gcttttgcgt gacctggctg gttcgtccgt tggatgacag agtggacatg
     2101 cgactcacct ccttccccgc aaaagaaaaa gcaagtgtcg taccgtctcg attctgtgac
     2161 cggtcgtctc cggcgcttcg aaacgagaat cgggatttca gctggttggt aatggtggca
     2221 ttgtttgccg gtttctacct aggcgtatct caggagatac agccaaggct tgggcgtatc
     2281 tatttccgta cagggcaagg tgtttcaacg gagcatgtac cgtggacgtg caggagatgt
     2341 cctgacacga gggtgttcag ggaacaagta tatggtcgat ggatgaaact gtggagcgaa
     2401 ggagcaggga tctacagttc gcgaatggcg ccttgtagga cggtcatctg agtcatgggg
     2461 tc
//
LOCUS       DAOQEW010000365         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_23550_length_2451_cov_7.046745, whole genome shotgun sequence.
ACCESSION   DAOQEW010000365 DAOQEW010000000
VERSION     DAOQEW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23550_length_2451_cov_7.046745"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..648)
                     /locus_tag="PLS35_20080"
     CDS             complement(112..648)
                     /locus_tag="PLS35_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21026.1"
                     /translation="MAQPHDSMQSDYSHLLTLWTAGVRDYHTMLSDYLTANSIFVAVI
                     GLLVSRESLAVPFTLVIVLLSAIGILMSVQMAIVLGRFSGQNALWEWQLRGIESMPDW
                     RERKPVSMLYRLREHRETIVDETNEPRSFEPSWAFRQHRQWWAHRAISFPWFFGTVYG
                     LFLLWAVTQMARSSMMFW"
     gene            complement(672..2375)
                     /gene="glgP"
                     /locus_tag="PLS35_20085"
     CDS             complement(672..2375)
                     /gene="glgP"
                     /locus_tag="PLS35_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNI21027.1"
                     /translation="MMQQNDKARVAYFSMEIGLNPLMPTYAGGLGVLAGDTIRSAADL
                     GVPMVGVTLLHRHGYFYQRLDRTGRQSEEPVAWAVTDFAEPLDAHVSVEIDGRNVQVR
                     AWRHLVTGMSGHTVPVYLLDTDVPENSSWDRTITDALYGGDAYYRLCQEMVLGFGGFD
                     LLRALGQGDIFRYHLNEGHAALLVLALMESAIGTAPVNGEVPPEVIEKVREQCVFTTH
                     TPVPAGHDQFPADLAHRVLGRRRCELAQACQHQGALNMTLLALRGSRFVNGVAMRHGE
                     VSHSLYPGYPIHSITNGVHAVAWAAPSFQNLYDRHLPDWRYDHLSLRYAISIPSSEIW
                     AAHREAKLALVDEVNRETNAGFDRDVLTIGFARRATAYKRATLVFQDIARLQRLMKAK
                     GQIQFVFAGKAHPQDEAGKDTIQRIHGMRGQLPVAYLANYDMTLACLLCAGVDVWLNT
                     PLPPMEASGTSGMKAAMNGVPSLSVLDGWWVEGHIEGVTGWSIGDKVEACLAPGSDME
                     ACHAAALYDKLEQQVLPCFYQDRERFIDIMKHTIAINGAFFNTQRMVQQYLRNAYRLV
                     G"
BASE COUNT          539 a          770 c          688 g          454 t
ORIGIN      
        1 catgtggtct tcatctctga ctcatgtagg aatgcgagaa aagaacagcg ggaaggatga
       61 ctcctcacag aagggaaagg agagggtgca ccgaggcgta caaccggcgg gtcaccagaa
      121 catcatgctc gacctcgcca tctgcgtcac cgcccacaac aaaaagaggc cgtagaccgt
      181 accaaaaaac catgggaagc tgatcgcccg atgcgcccac cattggcgat gctgccggaa
      241 tgcccaggac ggctcgaagg agcgcggctc attcgtctca tccacaatcg tttcgcgatg
      301 ctcgcggagc cggtacagca tactgaccgg cttccgttcc ctccaatccg gcatggattc
      361 aatcccgcgc agctgccatt cccacaaggc gttctgcccg gaaaaccggc ccaacacgat
      421 cgccatttgt acgctcatga gaatgccgat tgcgctcagt aggacaatca cgagggtaaa
      481 cggcaccgcc agggattccc gtgacacgag caggccgatg acggcgacaa agatggagtt
      541 ggcggtcaga tagtcggaca gcatcgtgtg gtaatcgcgc accccggcag tccagagcgt
      601 caacaagtgg ctataatcgg actgcatgct gtcgtgaggc tgagccatac gcctatgctc
      661 cggtggccgg tctatccgac cagtcgatac gcattccgca agtattgctg caccatgcgc
      721 tgcgtattga aaaacgcgcc gttgatcgcg atcgtatgtt tcatgatgtc gatgaatcgc
      781 tcgcggtcct ggtaaaagca gggcagaact tgctgctcca gcttgtcata caaggcggcg
      841 gcatgacagg cctccatgtc cgaccccgga gcgagacagg cttcaacctt gtcaccgatg
      901 gaccaacccg tcacgccttc gatatgcccc tccacccacc agccgtcgag cacgctgaga
      961 ctgggaacgc cgttcatcgc ggccttcatg ccgctcgtcc ccgaggcttc catcggcggc
     1021 agcggcgtat tgagccacac atccacaccg gcacagagca gacacgccag ggtcatatcg
     1081 taattggcca gatacgcaac gggcaattgt ccacgcattc cgtgtatccg ttggatcgtg
     1141 tctttcccgg cctcatcctg aggatgcgcc ttgcccgcga agacaaattg gatctgcccc
     1201 ttcgccttca tcaggcgctg cagccgcgcg atatcctgaa aaaccagtgt cgctcgtttg
     1261 tacgccgtgg cgcggcgggc gaatccaatg gtcagtacat cgcgatcgaa tccggcattg
     1321 gtttcgcgat tcacttcatc caccagcgcc agcttggctt cccgatgggc agcccagatt
     1381 tccgatgagg gaatgctaat ggcgtaccgg agcgacagat gatcgtatcg ccagtccggc
     1441 agatggcgat cgtacagatt ctgaaacgaa ggcgcggccc aggccacggc atggacgccg
     1501 ttcgtaatcg aatggatcgg ataaccggga tacagggaat gggaaacctc tccatgccgc
     1561 atcgccacgc cgttgacaaa gcgggaccct cgcaacgcga gcagggtcat gttcaacgcg
     1621 ccttgatgct ggcacgcctg cgccagctcg cagcgccggc gtccgagcac ccgatgcgcc
     1681 agatcggccg ggaactgatc atgccccgcc ggtaccggag tgtgcgtcgt aaagacacat
     1741 tgctcccgca ccttctcgat cacttcgggc ggcacctcgc cgttcaccgg tgcggtcccg
     1801 atagcggatt ccatcagggc aaggaccagc aaagccgcat gtccttcgtt gagatgataa
     1861 cgaaagatgt ccccctgtcc gagtgcgcgc aacaggtcaa atccgccgaa cccgagcacc
     1921 atttcctggc acaggcggta ataggcgtcg cccccataca gtgcatcggt aattgtccgg
     1981 tcccatgagc tgttttccgg cacatccgta tcgagaaggt agacaggaac ggtatggcct
     2041 gacatccccg ttaccagatg gcgccatgcg cggacctgca cgttgcgacc gtcgatctcc
     2101 acggagacat gggcgtccag cggctcggca aaatcagtca ccgcccaggc caccggctcc
     2161 tccgactgtc gtcccgtccg gtcgaggcgt tgataaaaat agccgtgacg gtgcagcaac
     2221 gtgactccga ccatgggtac cccaaggtcg gcagcggatc ggatcgtatc gcccgccaac
     2281 accccgagcc ctccggcata ggtcggcatc agaggattga ggccgatctc catcgaaaag
     2341 taggccacgc gagctttatc gttttgctgc atcatgatgc gttgagcact tgcttgatca
     2401 gccgtagttc atcacgcacc agccgcggca tcagaaaatg ccggcgcaca t
//
LOCUS       DAOQEW010000366         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_24089_length_2421_cov_7.906593, whole genome shotgun sequence.
ACCESSION   DAOQEW010000366 DAOQEW010000000
VERSION     DAOQEW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24089_length_2421_cov_7.906593"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLS35_20090"
     CDS             <1..276
                     /locus_tag="PLS35_20090"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNI21028.1"
                     /translation="DKTLRRRTVFSDHSLASDSVFAELHLISCRNVLIYFDRPLQDRA
                     VGLFKDALVRNGFLGIGARESLRFNAHAKAFREFLPVERIYQKRSDL"
     gene            273..875
                     /locus_tag="PLS35_20095"
     CDS             273..875
                     /locus_tag="PLS35_20095"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNI21029.1"
                     /translation="MSVLQHDLSLLDIEAIVIGASAGAIASLTQLLPPLGQDYPLPIL
                     VTVHVPPDQPSSIPSLLQAKCRVTIKEAEDKEFICPGAVYFAPPDYHVLVEKDRSLSL
                     SNEEPVSYARPSIDVLFESAADVYGKTLLALILSGANHDGAQGSRSVVKAGGTVLVQL
                     PESAAARIMPEATLAACPDARAMSLSDINQLLASREPRES"
     gene            872..>2421
                     /locus_tag="PLS35_20100"
     CDS             872..>2421
                     /locus_tag="PLS35_20100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNI21030.1"
                     /translation="MMPAKQSKLLLVDDHEANLVALRTLLSQDGVEVLQAGSGREALE
                     MLLDHDVALAIIDVHMPIMDGFELAELMRGSRRTQHVPIIFLTGESQDNLHRFRGYQA
                     GAVDFLYKPIEPHVLRSKAAIFLDLFRQREELARQRDRFLTLAEEKARLLRERDEADQ
                     RLRDSESRFRLLADSAPVIIWMNGLEGVEFVNQACLNFFGVATVEEASKLSWLHYVHP
                     DDQAQAADTYRRAVAERLRFETSFRCRRGDGVYRWMRTIGMPTLSPSEQLLGYIGASF
                     DLTESKEAEERLQRWSVDLERAVNQKTDELRRSQTQLRALATELNLAEQKERKRLATE
                     LHDYLAQLLVVVRMKLRQAIPGASGDRTAELLQDADQALTQSLDYTRCLVAELTPPTL
                     KEFGLLESLTWLAEQMQRHGLPVAMQQQMAALALPEDQAVLLFQSVRELLFNVLKHAK
                     ATQATVTVRIAEHHRLEIIVSDDGCGFVSTPSDHQGSGAARFGLFSITERMAAMGGDL
                     LIESAPGQG"
BASE COUNT          470 a          769 c          708 g          474 t
ORIGIN      
        1 gacaaaacgc tccggcggcg gacggtcttt tccgaccaca gcctcgcctc cgattccgtc
       61 tttgccgaac tgcatctcat ctcctgccgt aacgtcctga tctatttcga tcggccgctg
      121 caggatcgcg cggtgggcct ctttaaggat gcgctggtcc ggaatggatt cctgggaatc
      181 ggcgccaggg agagtctccg cttcaatgcc cacgcgaagg ccttccgcga atttctaccc
      241 gtggaacgga tctatcagaa gcggagcgac ctatgagtgt cctgcagcac gacctctcat
      301 tgctcgatat tgaagccatc gtcatcggcg catcggccgg ggcgatcgcc tctcttaccc
      361 aattgctccc tccgctgggg caggactatc ccttgccgat tctcgtcacc gtacacgtgc
      421 cgcctgatca gcccagcagt attccctcct tgctccaggc gaaatgccgg gtcacgatca
      481 aggaggcgga ggacaaggag ttcatctgcc ccggcgccgt gtatttcgcc ccgccggatt
      541 atcatgtcct ggtcgaaaag gaccggtcct tatccttgtc gaatgaggag ccggtgagct
      601 atgcgcggcc ttccattgac gtgctgttcg aatccgcagc cgatgtctac ggcaaaacgc
      661 tgctggcact catactcagc ggagccaatc acgacggcgc gcaagggtcc cgctcggtcg
      721 tcaaggccgg cggcaccgtg ctggtgcagc tgcctgaatc ggccgcggcg cgcataatgc
      781 ccgaagccac gctggctgcc tgtcccgacg cgagggccat gagcctgtcc gacatcaacc
      841 agctgttggc ctcacgggaa ccaagagaat catgatgccg gcaaaacagt ccaagctcct
      901 gctcgtcgac gatcacgaag ccaacctcgt ggcgctcagg acgttgttgt ctcaagacgg
      961 ggtggaagtc ctgcaagccg gctcaggccg cgaggccttg gagatgctgc tggatcacga
     1021 tgtggccctg gccatcatcg acgtgcatat gccgatcatg gatggatttg aactggccga
     1081 gctgatgcgc ggttcgcgcc gcacgcagca cgtcccgatc attttcctca cgggagaatc
     1141 gcaggacaat ctgcaccggt ttcgtggcta ccaggcaggc gccgtcgatt ttctgtacaa
     1201 accgatcgag ccgcatgtcc tgcgcagtaa ggccgctatc tttctcgatc tcttcaggca
     1261 gcgggaggag ctggctcgac agcgggaccg cttcctcacc ctcgccgaag aaaaagcccg
     1321 tctgttgcgc gaacgagacg aagctgacca gcgcctccgc gacagtgaat cccggtttcg
     1381 cctgctggct gacagcgcgc ccgtcatcat ctggatgaac ggcctcgagg gagtggagtt
     1441 cgtcaaccag gcctgcctga actttttcgg agtggccacc gtcgaagagg ccagcaagct
     1501 ctcctggctg cactacgtcc atcccgatga tcaggcccag gccgccgaca cctaccgccg
     1561 cgccgtcgcc gagcgcctcc gattcgagac ctcgtttcgt tgtcgtcgag gagatggggt
     1621 ctaccgctgg atgcgcacga tcggcatgcc gaccctctca ccgtctgaac agctcttggg
     1681 ctacatcggt gccagtttcg acctcacgga aagtaaggag gcggaggaac gcctccagcg
     1741 ctggagtgtc gacttggagc gcgcggtcaa tcagaagacg gacgaattgc gccgttcgca
     1801 gacacaactc cgtgcattgg cgactgaact caatctggcg gagcagaaag aacgcaaacg
     1861 gctggcgacc gaactgcatg actacctggc gcaactgctg gtggtcgtcc gcatgaaatt
     1921 gcgtcaggcc attcccggcg catccgggga tcgcacagcg gaactcctgc aagacgcgga
     1981 tcaggccctc acccagtcac tcgattacac ccgatgcctg gtcgcagaat tgacgccccc
     2041 cacgctcaag gagtttggtc ttctggagtc gctgacctgg ttggccgagc agatgcaacg
     2101 gcacggactt cctgtcgcga tgcagcaaca gatggcggcc ctcgcgcttc ccgaggacca
     2161 ggcggtcctg ttgtttcaat ccgtgcgtga attactcttc aacgtgctca aacatgccaa
     2221 agccacccag gcgaccgtga cagtcaggat cgcggaacac caccggttgg aaattatcgt
     2281 ctcggatgac ggctgcggtt tcgtctcgac tccatcggac catcagggaa gcggtgctgc
     2341 gcgcttcggt ctattcagca ttaccgagcg aatggcggca atgggcggtg acctcctcat
     2401 tgaatccgcg cccggccagg g
//
LOCUS       DAOQEW010000367         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_24163_length_2417_cov_4.513971, whole genome shotgun sequence.
ACCESSION   DAOQEW010000367 DAOQEW010000000
VERSION     DAOQEW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24163_length_2417_cov_4.513971"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLS35_20105"
     CDS             <1..1166
                     /locus_tag="PLS35_20105"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HNI21031.1"
                     /translation="GSVTAYAEVDRAFIGTHFHDAGKKPLAVSASIGSLRLWDARLGW
                     NYLEPAKGAWAFEAMDVAVADARQANLEILYPLGLTPKWASSDPERASAYGPGNSMPP
                     RLMADWEEYVRTVVRRYRGRIAAYEIWNEPNRALFFGGTTADLKRLTCSAYAIIKQID
                     PAAKVVGAAATDQMQGVRWLADFLDKEVLSCVDAIGFHLYTLAHEPPEAVVPIVREIR
                     NVLVSKGAASVPLWNTEFGWYIANSQVANDSRYKVLNASTARDFLLRSFVVQASLGIE
                     RAFQYAWNNRRMGVIEPDTGKVKDVGEAFAVAARWLGGADEVSCRESAGIYRCSVRRG
                     ITRNLIVWSVSGTRKVDRTELGGFNRVSFAGEQEVPLIGNEIEFSGTPALLRP"
     gene            1239..1940
                     /locus_tag="PLS35_20110"
     CDS             1239..1940
                     /locus_tag="PLS35_20110"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNI21032.1"
                     /translation="MSLDQKYDRMYGTATISDEHADADPRSWPRDRVAAILKDAPPAY
                     CVLDVGCGDGRLLLRLRDRFRQFVGLEYSRSRLDQSCANLSSLPFQPVLGSAEDMSGI
                     PAQSIDVIVSADVIEHVPDVYRAAEEMLRVLKPGGVAVINTPNVAFIKKRLLLAAGRF
                     PSTSQPNEGLGSDILFDGGHLHYFTFRSLSLLLVKAGFDIVRHTGFGVLGPASRLWPG
                     LLSSGVQVVARRPLA"
     gene            2002..>2417
                     /locus_tag="PLS35_20115"
     CDS             2002..>2417
                     /locus_tag="PLS35_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21033.1"
                     /translation="MTHYRVPLFERMREMLSARGIRLRLLYGDPTQGERAKNDAGHVE
                     WAERLESVYLFGDRVCWQNFHRATGSSDLVIATQENKLVFNLLALSLLRPRRFAFWGH
                     GKNMQASRTDHWTEQFKRWTTRRVDWWFAYTALSRS"
BASE COUNT          455 a          685 c          767 g          510 t
ORIGIN      
        1 cgggcagcgt aactgcttac gctgaggtgg atcgcgcgtt catcggtacg cattttcatg
       61 atgccggcaa gaagccgctt gcggtcagtg cgtcaatcgg gtcattgcga ctgtgggatg
      121 cgcgcttggg atggaactat ctcgagcccg cgaaaggcgc ctgggccttc gaggcgatgg
      181 atgttgccgt cgcagatgcg cggcaagcca atctagagat tctctatccg cttggcctca
      241 cgcccaagtg ggcgtcctcc gatccggagc gcgcctcggc atacgggccg ggaaactcca
      301 tgcctccaag gttgatggca gactgggaag agtacgtccg aacggtcgta agacgatacc
      361 gcgggcgcat tgcagcgtat gaaatctgga acgagccgaa tcgcgccctg ttctttggag
      421 gaaccacggc ggatctgaag cgattgacct gctccgcata tgcgatcatc aaacagatcg
      481 atccggccgc caaagtggtg ggagcagcgg cgacagacca gatgcagggc gtgcgctggc
      541 tggcggattt cctcgacaaa gaggttcttt cctgtgtcga tgcgatcggt ttccacctct
      601 acaccctggc gcacgaacct cccgaagcgg tggtgccgat cgttcgcgag attcggaacg
      661 tgttggtctc gaaaggggct gcctcggttc cgctctggaa taccgagttt ggctggtaca
      721 tcgcgaactc acaggttgcc aacgactcgc gctacaaggt gctgaacgcc tcgacggccc
      781 gcgatttcct gctgcgctcg tttgtggtcc aggcttctct cgggatcgag cgcgcgttcc
      841 agtacgcctg gaacaaccgc agaatgggtg tgatcgaacc ggatacggga aaggtcaagg
      901 acgtgggcga agccttcgca gtcgcagcgc gctggctcgg cggtgccgac gaggtgtcgt
      961 gtcgcgaaag cgcggggatc tatcgatgca gcgtccggcg cgggattaca aggaacctga
     1021 ttgtgtggtc ggtgtccggt acgcggaaag ttgatcgaac cgagctgggc gggttcaatc
     1081 gtgtttcctt tgccggcgag caggaggtgc ccctgattgg caatgagatc gaattctccg
     1141 gcacgcctgc acttctgcgt ccctaatggc agccgctgtg cacgttgcct ggccgcgtac
     1201 ctgcaatgtc ccatgcttga tcggaacgcg ggaaaacaat gagccttgat caaaagtacg
     1261 atcgaatgta cggcacagcc acgatatcgg acgagcacgc ggatgcggat ccacgatcgt
     1321 ggccgcggga cagggtggct gcgatattga aggacgcgcc gcctgcatat tgcgtgctcg
     1381 atgtcggttg tggcgacgga cggctactgt tgcgcctccg agaccggttc cgccaattcg
     1441 tcggcctcga gtactcccgg tcccggctcg atcaatcatg cgcgaacctt tccagcctgc
     1501 cgtttcaacc cgttctcggc agcgcggaag acatgtccgg gattccggca caaagcatcg
     1561 acgtcattgt cagtgcagac gtcatcgagc atgtgccgga tgtctatcgc gccgccgagg
     1621 aaatgctgcg cgtacttaaa cctggcgggg ttgcggtgat caatacgccg aatgtggcct
     1681 tcatcaagaa gaggttgctg ctcgcggccg gacggtttcc gtcgacgtcc caacccaacg
     1741 aagggctcgg aagtgacatc ctgttcgatg gcgggcacct gcattacttt acttttcgtt
     1801 cgttgtccct gcttctcgtg aaagccgggt tcgatattgt ccggcacacg ggtttcggcg
     1861 ttctcgggcc cgcgagccgc ctctggcccg ggcttttgag ttccggcgtg caggtcgttg
     1921 cgcgacgacc gttggcatga tggtccgatc cgttcgatgc tgatgccgat gtcaccggaa
     1981 gtctccatca tccagcgtcg catgacacat tatcgagtcc cgcttttcga gcgcatgagg
     2041 gagatgctgt ccgcgcgggg aatccgtctg cgactcttgt acggtgatcc cacacaaggc
     2101 gagcgcgcaa agaacgatgc ggggcatgtc gaatgggccg aacgtctgga gagcgtgtat
     2161 ctattcggcg acagggtgtg ctggcagaac tttcaccgtg caaccggaag ctcggatctc
     2221 gtgatcgcga cacaagaaaa caagctggtg ttcaatttgt tggccttgtc gctcttgcgg
     2281 ccacgccgtt tcgcgttttg ggggcacggg aaaaacatgc aggcttcccg aacggatcac
     2341 tggacggaac aattcaaacg ctggacgacc cgccgggtcg actggtggtt cgcgtacacg
     2401 gcgctctccc ggtcctt
//
LOCUS       DAOQEW010000368         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_24264_length_2411_cov_8.539474, whole genome shotgun sequence.
ACCESSION   DAOQEW010000368 DAOQEW010000000
VERSION     DAOQEW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24264_length_2411_cov_8.539474"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLS35_20120"
     CDS             <1..231
                     /locus_tag="PLS35_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21034.1"
                     /translation="HRSPPNEMSCSTRNNEEPLFLTDPETGKGVSLTLWDSLEDMKAG
                     ESSGYLQEQIAKFAPLMAGPPTREVFVVAAQA"
     gene            complement(362..1354)
                     /locus_tag="PLS35_20125"
     CDS             complement(362..1354)
                     /locus_tag="PLS35_20125"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNI21035.1"
                     /translation="MSSYRGRFPCGYAPGLVLSVRKSPEGGGVRTQVVQCPQRTERVD
                     DLVLLVHGFNNHVCEAAERYKAFRDRQYPLAGMQPPQLERTLGDFYWPGDAEWGKADL
                     FDFAAYPQMLQQALDSAKSLAKYLRERREFQKIHFVGHSLGCRVVLECIREMLPTCAG
                     RIGHVILMAPAVPTAMVERGAELAAAVERAESVLVLSSFSDGVLCFSFPAGETLVGQR
                     EGILPQALGRDGPPSNKPGNLTGEPIDSASHGDYWGFKPICEAASQKVADLVAQTLWP
                     GCARPEPPCREVGAGRECGSPRSESAPSRGILSSRANGYCQAERNLADCLAFPS"
     gene            complement(1351..2319)
                     /locus_tag="PLS35_20130"
     CDS             complement(1351..2319)
                     /locus_tag="PLS35_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21036.1"
                     /translation="MLISNDQGSKSDKSHYRVEHLARRSLKNRPPFDPEQCYEELLQL
                     TTGLGPGESKQVSRFESSNSSDQALFGAGSFNRSIEYALFELDLDRMMGQIQEAGDVA
                     LNNPGQYPNLFMFLTRGARREDVEAWWKYLVPAKAVVEKSRSSDAEVPDLDRMMSQIQ
                     AAADMALNNPEDYQDLFRFLTRSAREEDVEAWRKYLATEKGVADKPGEPDPNAPNGKD
                     LAERYTRIKQAVRRHLDGFQIVTAMRWREWNQFAALVLGGSLMLLAQMIEIIPAPSIP
                     APPCVGSVILKKAWLDPLGMIVFSLLGGILAPIAKDLVDALNKVKP"
BASE COUNT          506 a          780 c          667 g          458 t
ORIGIN      
        1 caccggtcac ctccaaatga gatgtcgtgt tccacacgaa acaacgagga gccactcttc
       61 ctgaccgacc ccgaaaccgg caagggcgtg tcgttgacgc tctgggacag cttggaggac
      121 atgaaggcgg gcgagagcag cggctatctg caggagcaga tcgcgaagtt cgccccgctg
      181 atggccgggc cgcccacgcg cgaggtcttc gtggttgcgg cccaggccta gccgaaccgg
      241 agggtggcgg cgcggtggac ctcgcgcagg cccgtaccgc gctgcaggcc gggagcgagg
      301 acttggatgc gctcgcatcc gagtcaccgt acacgcattg ccgacctacc acatcgtccg
      361 ttcaactcgg aaaagcaaga cagtcggcaa gatttcgctc ggcttggcag tatccattcg
      421 cgcgggacga caggatgcct cgagatggcg ccgactcact gcgtggggat ccgcactccc
      481 gtccggcgcc gacttcacgg catggcggtt ctggccgggc acagccaggc caaagggttt
      541 gtgccaccag atcggcgacc ttttgactcg ctgcctcaca tattggcttg aagccccagt
      601 agtcgccgtg gctcgcagaa tcgatgggct cacctgttaa attccctggc ttgttggaag
      661 gcggcccatc ccgaccgagc gcctgcggca aaataccctc cctctgcccc accaaggtct
      721 cacccgccgg gaatgaaaaa cacagcacac cgtcggagaa cgagctgagc accagcaccg
      781 attcagcccg ctcgaccgcc gcggccaact cagcgccccg ttcgaccatc gcggtcggca
      841 ccgccggagc catcaagata acatgtccga tccgacccgc acaggttggc agcatctcgc
      901 gaatgcactc cagcaccacc cgacaaccaa gagagtgccc gacgaaatgg atcttctgga
      961 actcacgccg ttcacgcagg tacttcgcga gactcttggc tgagtccaac gcctgctgaa
     1021 gcatttgagg gtaggctgca aagtcgaata gatccgcctt gccccattcc gcatcaccgg
     1081 gccagtagaa gtcgccaagg gttcgctcca gctgcggagg ctgcattccc gccagcgggt
     1141 attgacgatc gcgaaaggct ttgtagcgct ccgccgcttc gcagacgtga ttgttgaacc
     1201 cgtgcaccag cagcaccaag tcgtcgacgc gctcggtgcg ctgcgggcat tgcacgacct
     1261 gcgtccgaac accgccgcct tccggcgatt tccgcacgga cagcaccaac ccgggcgcat
     1321 acccacaggg aaatctcccg cgatagctgc tcacggcttc accttgttca gtgcgtcaac
     1381 cagatccttc gcgatgggtg ccaatatgcc gcccaacaga gaaaacacaa tcataccgag
     1441 cgggtccagc catgcttttt tcaaaataac cgagcccacg cacggaggtg cagggatgct
     1501 aggtgccggg atgatttcaa tcatctgtgc caacagcatc aacgatccac caaggaccaa
     1561 tgccgcaaat tggttccatt cacgccagcg catcgccgtg acgatctgga agccgtcgag
     1621 gtgccggcgt acggcctgct tgatgcgcgt gtagcgctca gccaagtcct tgccgttcgg
     1681 cgcattggga tctggctctc ctggtttgtc ggcgactccc ttttctgtcg ccaagtactt
     1741 ccgccaggcc tcgacatctt cttcgcgggc gctgcgcgtc aagaacctga agagatcctg
     1801 ataatcctcc ggattgttca acgccatgtc cgctgctgcc tggatctggc tcatcatgcg
     1861 gtcgagatcc ggcacctccg catccgacga cctggatttc tcgaccacag ccttcgcagg
     1921 caccaagtac ttccaccagg cttcgacatc ctccctgcgc gcaccgcgcg tcagaaacat
     1981 gaagagattg ggatattgac ccgggttgtt caaggcaacg tctcccgcct cctggatctg
     2041 ccccatcatg cgatcgagat ccaactcgaa cagcgcatac tcgatcgaac ggttgaacga
     2101 acctgcaccg aacagcgcct gatcgctgct gttcgaggac tcgaaacggg agacctgctt
     2161 cgattcaccg ggaccgaggc cggtcgtcaa ctgaagcagc tcctcataac actgctcggg
     2221 gtcgaacggc ggtcggttct tgagtgacct gcgcgcaagg tgctccacgc gataatggct
     2281 cttgtcggac ttgctgccct gatcattgct tattaacatg gcaattaaat acagaattaa
     2341 ctataatagt tgaattacca aagaagcaga cgcccgttca ctgtcggaag aatcccttga
     2401 gttgttgcgc t
//
LOCUS       DAOQEW010000369         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_24442_length_2402_cov_6.348956, whole genome shotgun sequence.
ACCESSION   DAOQEW010000369 DAOQEW010000000
VERSION     DAOQEW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24442_length_2402_cov_6.348956"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLS35_20135"
     CDS             <1..716
                     /locus_tag="PLS35_20135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNI21037.1"
                     /translation="ELCAARYQFSRQQVDDFAMESYSRAREAITAGSFTREIVPVEVP
                     QKKGAPLMIADDEEPNRVDLSRLRQLKPVFQDDGVLTVGNSPSCNDGAAALVVMAEEE
                     AHRLGLPPMARIVGYAGAALAPEWFTIAPIDAIRRLLKQTELTVSDIDLFEINEAFSA
                     VSLAITRELGLDAKKVNVNGGAVALGHPIGATGARILTTLLYAMEERDAHRGIASLCI
                     GGGEALALLVERPRKIQHQ"
     gene            742..1638
                     /locus_tag="PLS35_20140"
     CDS             742..1638
                     /locus_tag="PLS35_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNI21038.1"
                     /translation="MESRMTIDDITTVGVVGAGQMGRGITQVLATAGWKVLLVDVTEE
                     ALEDATKKIWQGVTKAVEKGALRGDQAGSAMAIIHPIRHMQRLREAQVVIEAIPEDPV
                     MKRALFAQLGQICEPSTLLASNTSSISIASLGMVSGRPDRVVGIHFMNPVPVMRLVEV
                     VRAIGTSEQTMRLALELVQRAGKTAVVCKDFPGFIVNRVLIPMINEAIFALEDGVATA
                     EAIDLAMVEGTNHPVGPLALADRIGLDTVLAICEVLHQDLGDPKFRPCPLLRKYVEAG
                     WLGRKSGHGFYQYGDQPVMHLT"
     gene            1670..>2402
                     /locus_tag="PLS35_20145"
     CDS             1670..>2402
                     /locus_tag="PLS35_20145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNI21039.1"
                     /translation="MNERKSRFTSLSGLEIERCYGPDDLKGWSAEKDLGQPGAFPYTR
                     GCYPNMYRSRLWTMRQFAGFGSADDTNTRFKYLLDHGQTGLSVAFDLPTLMGLDSDDP
                     LARGEVGYCGVAISSLADMERLFDGIPLDQVTTSMTINGPAAVLFAMYLAVAEKRGIP
                     FERLGGTIQNDILKEYIAQKEWLFPPEPHLTLIVDTIAYCAAHVPKWHPISISGYHIR
                     EAGSTAIQELAFTLYNGLTYVEAAVK"
BASE COUNT          501 a          699 c          741 g          461 t
ORIGIN      
        1 gagaactctg cgccgccaga tatcagtttt cacggcagca ggtcgacgac tttgccatgg
       61 aaagttacag ccgcgcgcgt gaagccatca ccgccggttc attcaccagg gagattgttc
      121 cggtcgaggt gccccagaaa aaaggcgcac cactcatgat cgcagacgat gaggaaccga
      181 accgggtcga tctctcaagg ctgcgacagt tgaaacctgt ctttcaggac gacggagtgc
      241 tgacggtggg taactccccg tcctgcaatg acggggcggc agcattagtc gtcatggcgg
      301 aggaagaggc ccaccggctt ggattgcccc ccatggctcg catcgtggga tatgccggag
      361 ctgcgctcgc gccggaatgg ttcaccattg cgcccatcga cgcgattcgg cggctcctga
      421 aacagacgga gttgacggtc agcgacatcg acctttttga aatcaacgaa gccttttcgg
      481 cggtctcctt ggcgatcacg cgtgaactcg gattggatgc caagaaggtc aacgtcaacg
      541 gcggcgcggt cgcgctgggt catcccatcg gcgccaccgg cgcacggatc ctgaccacgc
      601 tgctctatgc gatggaggaa cgggatgccc atcgcggaat cgcgagcctc tgcattggcg
      661 gcggtgaggc actcgcactg ctcgtggaac ggccgagaaa aatacagcat cagtgatcac
      721 acgtgcgcaa gagcagcagc catggaatcg cgaatgacca tcgatgacat caccacagtc
      781 ggcgtcgtcg gcgcggggca aatgggacgc ggcatcaccc aggtgttggc gacggctggc
      841 tggaaagtgt tgctggtgga tgtgacggaa gaggcgctcg aggacgcgac caaaaagatc
      901 tggcagggtg tcacgaaggc ggtggaaaaa ggcgcgctgc gaggtgatca ggccggatcg
      961 gccatggcca tcatccatcc gatacggcac atgcaacgcc tgcgtgaggc gcaggtggtg
     1021 atcgaagcga taccggaaga tccggtgatg aaacgggccc tcttcgcgca attgggacag
     1081 atctgcgagc cctcgaccct gttggcgagc aatacctctt caatttcgat cgccagcctc
     1141 ggcatggtgt cagggcggcc cgaccgggtc gttggcatcc atttcatgaa ccctgtgccg
     1201 gtgatgcgat tggtggaagt cgtccgcgcc attggcacgt cggaacagac gatgcgcctg
     1261 gcgcttgaac tcgttcaacg cgcagggaag acagccgtcg tctgcaagga ttttcccggc
     1321 ttcatcgtca atcgggtgct cattcccatg atcaacgaag ccatttttgc gctggaggat
     1381 ggggtcgcga cggcggaagc catcgatctg gcgatggtgg aagggaccaa ccatcctgtc
     1441 ggtcctttgg cactggcaga tcggatcggc ctggatactg tactcgccat ctgcgaggtg
     1501 ctccatcagg acttgggtga tccgaaattc cgcccctgcc cgctgttgcg gaaatacgta
     1561 gaagccggct ggcttggcag gaaaagcgga catggatttt atcagtacgg agatcagccg
     1621 gtaatgcacc tgacctgacg tttcctgtcc gtgattggag gaacgcgtca tgaacgagcg
     1681 gaaatcccga ttcacgtccc tgtccggctt ggagatcgag cggtgttacg gtcccgatga
     1741 tctcaagggc tggagcgccg agaaggatct cgggcaacct ggggccttcc cttacacccg
     1801 cggctgttat cccaacatgt atcgcagccg gctctggacc atgcggcaat ttgccgggtt
     1861 cggctcagcg gacgacacca acacacgctt caagtatctg ctggatcacg gccaaaccgg
     1921 cctgagcgtg gccttcgacc tgccgaccct catgggtctc gactctgatg atccgctcgc
     1981 ccgcggcgaa gtcggatatt gcggggtcgc catttcctcc ctcgctgaca tggagcgact
     2041 gttcgacggc attccgctcg accaggtcac tacctccatg acgatcaacg gacccgccgc
     2101 cgtcctcttc gccatgtatc tggccgtggc agaaaaacgc gggatcccgt tcgagcgact
     2161 gggcggcacg attcagaacg acattctgaa ggagtacatc gcccagaaag aatggctgtt
     2221 tccgccggaa ccgcatctga ccttgatcgt cgacaccatc gcgtattgtg cggctcatgt
     2281 cccgaagtgg catccaatca gcatcagcgg gtatcacatc cgggaagcag gctcgacagc
     2341 gatccaagaa ctcgccttca cgctctataa cgggctgacg tacgtcgagg cggccgtgaa
     2401 gg
//
LOCUS       DAOQEW010000370         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_24858_length_2380_cov_7.643441, whole genome shotgun sequence.
ACCESSION   DAOQEW010000370 DAOQEW010000000
VERSION     DAOQEW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24858_length_2380_cov_7.643441"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLS35_20150"
     CDS             complement(<1..840)
                     /locus_tag="PLS35_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21040.1"
                     /translation="MRPTLVPIHRPRVGISVSASALSLVALRRPWFRRPIVKQVVERP
                     LPSGLLKVSATEPHITDLDAFAQELRALTEGLSERTVALSVPNRAMHMGVFAFDRFPD
                     APEERAGLIKWRFREDLNLTIGDARVMLRVFPTGTRTQVLAVAVRQTILDQYEDVCGR
                     AGLIPVSMGFSHLQLIGLYRRLMKGTRRLCVMHWAGDEFMLLLFDQGQPVFLRTRSMT
                     SGTQDLRRELVGTLQYMADQATTVHETGESSFPLYLIGGQSNASITQLLGGEATVTVP
                     GYRG"
     gene            complement(845..>2380)
                     /locus_tag="PLS35_20155"
     CDS             complement(845..>2380)
                     /locus_tag="PLS35_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNI21041.1"
                     /translation="GVPYHSLHEFRVDFESFPSIPVDVMRQFACVPIGEEEGAFLLAL
                     ADPTDLPRLDQLERALGRSCRIHVAPRSAIAESLRESTRNSQVVAKIKDEFRPLLVRE
                     NDEGEEVLSVEKVQRDLSPIVQLVDTIILNALQKRASDIHLEPADGVTEIKYRIDGVL
                     YLAMEPLDLRFHNPLVSRIKVMSDLDIAERRVPQDGRFKLRLEKRTIDFRISVIPSAF
                     GESVVIRILAKEEFAAKRNGLHLDSLGLKKDDLDRFRRAITASYGMVLVTGPTGSGKT
                     TTLYAALNEVNTKEDKIITIEDPVEYQLAGVVQIPVNEKKGLTFAKGLRSILRHDPDK
                     IMVGEIRDAETAQIAIQSALTGHLVFSTVHANNAFDVIGRFVNMGIEPYNFVSSLNCI
                     MAQRLVRTICESCKAPHTVSQAQCEQLGFDYELFKDRPVYEGKGCLDCHGLGYRGRHA
                     ITEFLPLTDRIRELMLDRRPPSEIRRAAVEQGMTDLRQAGVEKVRRGETTMREVNRMT
                     FLG"
BASE COUNT          509 a          740 c          648 g          483 t
ORIGIN      
        1 acctcgatag ccaggcaccg tcaccgtcgc ctcccctccg agcagctgcg tgatcgatgc
       61 gttcgactga ccaccgatca ggtacaaggg aaacgaggac tcacccgttt catgcacagt
      121 cgtcgcctgg tcggccatat attgtagggt tccgaccagt tcgcgtcgaa gatcctgcgt
      181 cccgctggtc atggatctgg tgcgaagaaa cactggttgc ccctgatcga acagcagcag
      241 catgaactca tcccctgccc agtgcatcac gcacagccgt cgagtgccct tcatcagacg
      301 acgatagagg ccgatgagtt ggaggtgcga gaatcccatt gagacaggga tgagcccagc
      361 ccgtccgcag acgtcctcat attggtccag aattgtctga cgcaccgcca cggccagtac
      421 ctgggtgcgt gtcccggtgg ggaagacccg cagcatcacc cgagcgtcgc caatcgtcag
      481 gttgaggtcc tcgcgaaacc gccacttgat cagacctgct cgttcttcgg gcgcgtcagg
      541 gaatcgatca aacgcaaaca cccccatgtg catcgcgcga ttgggcacac tcagcgcgac
      601 cgttcgctcg ctgagccctt cggtcaacgc ccgcagctcc tgcgcgaagg catccagatc
      661 ggtgatgtgc ggctccgttg cggagacctt cagcaagcca ctgggcaacg gacgctccac
      721 tacctgtttc acgattggcc tgcggaacca cggccggcgc agagccacca gactcagtgc
      781 ggaagccgac acgctgatgc cgacacgggg acgatggatg ggaacgagtg tgggcctcat
      841 acggctatcc caaaaatgtc atccgattga cttcgcgcat cgtggtctcc cctcgtcgta
      901 ccttctccac gccggcttga cgcaaatcgg tcatcccctg ttcaaccgcc gcgcgcctga
      961 tctcagacgg cggccgccga tctaacatca gctcccgaat ccgatcggtg agcggcagaa
     1021 actctgtaat ggcatggcgc ccgcgatacc ccaatccatg acagtccagg cacccttttc
     1081 cttcatacac gggacggtcc ttgaagagct cgtaatcaaa ccccagttgt tcacactgag
     1141 cttgagacac ggtgtgcggg gccttacagg attcacagat ggtgcggacc agacgttggg
     1201 ccatgatgca gttcagagac gagacgaagt tatagggctc gatccccata ttcacgaacc
     1261 ggccaatcac atcaaaggca ttgttggcat gcacggttga gaagactaag tgtccggtca
     1321 gggccgattg gatcgcaatc tgcgccgtct cggcatcacg gatttcaccc accatgatct
     1381 tgtccggatc atggcggaga atcgaccgca atccctttgc gaaggtgagc cctttcttct
     1441 cattcacggg gatttgcacg acgcccgcca actgatattc gacgggatct tcaatcgtaa
     1501 tgatcttgtc ctctttcgta ttcacttcgt tcagagcggc atagagtgtg gtcgtcttcc
     1561 cgctgcccgt cggcccggtg accagcacca tgccatagga ggccgtgatg gcgcggcgga
     1621 accggtccaa atcgtctttc ttcagtccca gcgagtcgag atgcagaccg ttccgcttcg
     1681 cggcaaattc ctctttggca agaatacgga tgaccaccga ttccccgaac gcgctcggga
     1741 tcacggagat acgaaaatcg atggtgcgct tctcaaggcg gagcttaaac cgcccatctt
     1801 gtgggacccg acgttccgca atgtcgagat ccgacatgac tttgatgcgc gagacgagcg
     1861 gattgtggaa gcgcagatcc aagggttcca tggccaggta caacacgccg tcgatgcgat
     1921 acttaatctc cgtcacgcca tccgccggct cgagatggat atcactggct cgtttctgca
     1981 gcgcgttgag gatgatcgta tcgaccagtt ggacaatcgg actcagatcc cgttgcacct
     2041 tctccacgga gaggacttct tcgccttcat cgttctcacg caccaacagc ggacggaatt
     2101 catccttaat tttggcaacc acctggctat tccgcgtact ctcacgaagc gactcagcga
     2161 tggcagaacg cggtgccaca tggattcgac aactccgccc aagcgctcgt tccagttgat
     2221 caagccgcgg caaatcagtg gggtccgcca gcgccaacag gaatgctccc tcctcctctc
     2281 caatgggtac gcatgcgaac tgccgcatga cgtcgactgg tatcgagggg aacgattcga
     2341 agtccacccg gaactcgtga agcgagtggt agggcactcc
//
LOCUS       DAOQEW010000371         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_25241_length_2361_cov_6.260191, whole genome shotgun sequence.
ACCESSION   DAOQEW010000371 DAOQEW010000000
VERSION     DAOQEW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25241_length_2361_cov_6.260191"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1112
                     /locus_tag="PLS35_20160"
     CDS             45..1112
                     /locus_tag="PLS35_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNI21042.1"
                     /translation="MGRVAVALSCTAEVKRELERMSRSRSGEVRLAERARIVLGCLQG
                     LQNEEVAREVGVRPNTVGLWRRRFAEQGLSGLRDAARSGKPAKYGKDLRDRILTQLEL
                     TPPEGRASWDGGSLAKVLAVSDDAVWRVLRREGIQLQRHRSWCVSTDPDFAAKAADVI
                     GLYLNPPEKAMVLSVDEKPSIQALERRRGYVQTSSGKVVQGLQSTYKRHGTVNLFAAL
                     EVATGVIRGKTTPTQKRADFQAFMAEVVADEPADREIHVILDNLNTHQKNDEWLAAHP
                     NVTFHFTPTSASWLNQVEIWFGIFQRKTLRNASFSSVDQLVEAIHQFTTAYNQNAAPF
                     VWRKREVKGTQLRNTIVNLCN"
     gene            1115..1437
                     /locus_tag="PLS35_20165"
                     /pseudo
     CDS             1115..1437
                     /locus_tag="PLS35_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612142.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1822..>2361
                     /locus_tag="PLS35_20170"
     CDS             1822..>2361
                     /locus_tag="PLS35_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21043.1"
                     /translation="MKQISYTCALLLLMLGLPSIGVAGMFRQYSDALNETVTLVKTGE
                     LKQAVAKIENFNEGNDKDILYFFEKGELLSLGSNFISSRETWMKSDEIIQAWENEFRS
                     NPSKIFGDIGSFLISDKTRRYDGQDYEKVLLSTRLTMNHIMLGNFDHARIEMKKTYER
                     EKLIEAFREKEYDALKVESN"
BASE COUNT          568 a          667 c          678 g          448 t
ORIGIN      
        1 tagtaatatg atacctcccc atgcttttct ggtgggagaa ggaaatggga cgtgtggcgg
       61 tagcgctgtc ctgcacagca gaggtaaaga gggagttgga acgaatgtca cgcagtcgca
      121 gcggcgaggt tcgtttggcc gagcgagcga ggatcgtcct gggctgtttg cagggtctgc
      181 aaaacgagga ggtcgcccgc gaagtcgggg tacggccgaa caccgtgggg ttgtggcgtc
      241 ggcgctttgc cgagcagggc ttgtccgggt tgcgtgatgc ggctcggtcg ggcaagccgg
      301 cgaagtacgg gaaggatctg cgcgaccgta tcctgacgca gctggaactg actcctccgg
      361 aggggagggc gagttgggat ggggggtcat tggcgaaggt gctggcggtg tcggacgacg
      421 ccgtatggcg cgtattgcgt cgggagggca tccaactgca gcgccatcgc tcctggtgtg
      481 tcagcacgga ccccgatttc gcggccaaag ccgcggatgt cattggactg tacctgaacc
      541 caccggaaaa ggcgatggtt ctgagcgtcg acgagaagcc ctcgatccag gctctggagc
      601 gccgacgcgg ctatgtccag accagtagcg ggaaggtggt ccagggactc cagagcacct
      661 acaagcgcca tggcaccgtt aatctgttcg ccgccctgga agtagccacc ggcgtcatcc
      721 gcggcaagac cacaccaacc cagaagcgcg ccgacttcca ggccttcatg gcggaggttg
      781 tcgctgacga acccgccgac cgtgaaatcc acgtcatcct ggacaacctc aatacgcacc
      841 agaagaacga cgagtggctg gctgcccacc ccaacgtcac cttccacttc acacccacca
      901 gcgccagttg gcttaaccaa gtggagatct ggttcggcat cttccaacgc aagacgctgc
      961 gaaatgccag cttttcaagt gtcgatcaac tcgttgaggc cattcaccaa ttcaccaccg
     1021 cttacaacca gaacgccgct cccttcgtct ggcgaaagcg cgaggtcaaa ggcacgcagt
     1081 tacggaatac tattgttaac ctatgcaatt aaacactagc ctcttgaagg cgcacgacct
     1141 ccggatcagc atggacggcc aggggtcctg gcgggaaaac aagcgaagcg cagtttcgag
     1201 agccgatagg cgagagggcg aggcccgtca caacgtcttt gtcgagcgcc agtggaaaag
     1261 cgtccagtac gaagaggggt atctcaaggc ctacgaccgc gtcgccaatg cgaaagccca
     1321 ttggccacgt atttgcactt ctacaacgag cgacgacccc ctcgctcctt cgatggcagc
     1381 acaccggacg cgactgactt cgcggtactc gcggccgcca agcgaccggc ggcgtaaggc
     1441 atgagcaagg ggctccgtgg atttgtggac gatgcgctgc cgcgcaccgg gccgcttgcc
     1501 gtggaaaagt ctgacgacct ttccaccacg cgtcccttcg cccaccagct ccaccgagct
     1561 ctattcgttt tgtatagaat ccaggaaagt caaaacccac cgcgataccg gtcaatcgac
     1621 cacaacctgc agggctccac ttatccagga gcatttcctg tccaaacaag cggggccacc
     1681 tctttcggat cgcagcaggc catggcttat actgcggtgg ggtctaactt acttaaggag
     1741 cgcctctata gggtagtcac cgggatctga cagtcccatt accccagaga cataattaca
     1801 aacaccatcg gggagacaac catgaagcag atttcctata cctgtgcatt gctgttacta
     1861 atgcttggac taccttccat cggcgtcgcg ggcatgtttc gccaatactc cgatgcgctt
     1921 aacgaaaccg tcacactggt gaaaacaggc gaactcaaac aggctgtggc aaagatcgaa
     1981 aacttcaacg agggcaatga caaagacatc ctatactttt tcgaaaaggg ggaactactg
     2041 agtctcggca gcaacttcat cagcagtcgc gaaacctgga tgaaaagcga cgagatcatt
     2101 caggcgtggg aaaacgaatt tcgtagcaat ccatcaaaga tatttggtga cattggcagt
     2161 tttctaatta gcgacaagac ccgtcgttat gatgggcagg actacgaaaa ggtgctgctc
     2221 tcgacgcgcc tgaccatgaa tcacatcatg ctcggtaatt tcgaccatgc ccgcatcgaa
     2281 atgaagaaga cctatgaacg tgaaaaactg atcgaggctt tccgagaaaa ggagtacgac
     2341 gcgctcaagg ttgagtcaaa c
//
LOCUS       DAOQEW010000372         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_25510_length_2347_cov_2.947644, whole genome shotgun sequence.
ACCESSION   DAOQEW010000372 DAOQEW010000000
VERSION     DAOQEW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25510_length_2347_cov_2.947644"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLS35_20175"
     CDS             <1..552
                     /locus_tag="PLS35_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21044.1"
                     /translation="AEEGRPANAAFKHALEALAQHQASKPGFWANLCSLWGASRTWNT
                     ALKLLQDRHGLAKAEFDRIARLAKQFEDSRQQLDRQIANAQQALKKMRAEDQSLTQQA
                     SDLASACRADHLLAWLRGGVIGRGDAIELAEPWHIEGWRQARARVFIQALKLHRTFFE
                     LEAARMRSKPSFRSSASTAAVGM"
     gene            1247..2293
                     /locus_tag="PLS35_20180"
     CDS             1247..2293
                     /locus_tag="PLS35_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21045.1"
                     /translation="MQNPNNATFHYCCIYAAKGIIDPNQKLDSALLKDENIVVRLLGS
                     DAYENLVWRIVSPAGKKGIEDLDDKAREKLELRRNSLILQALHHAVIHVESCLPLDIV
                     RIYQEENSELPAYVKAPLKKNAERAFKSVSAAMTFARGNFLSKNYDFIHEQYYVTDDQ
                     STVIQKLHPATISDGDLKTKLSMEEVQVTEKLASILHHDKEINKIIDIYSESLTPYKS
                     GHLHAFIAAWTALEMFVATQFKEIQSSVAININGTAAHNDFSSRMLAVMKDKYRLVDK
                     FAALSSYYEDADADEGIAVLKSIKEIRDKFFHTMEGEIKDLPLDQTRELISKYLQIYL
                     ERKCEGFVFQKAAR"
BASE COUNT          611 a          574 c          670 g          492 t
ORIGIN      
        1 gccgaggaag gtcgtcccgc caatgcggcg ttcaaacacg cgctggaggc gcttgcacag
       61 caccaggcca gcaaaccagg gttctgggcg aacctatgca gtctgtgggg agcgtcacgc
      121 acctggaaca ctgcactgaa actgctgcaa gaccggcatg gcctcgccaa ggccgagttc
      181 gaccgcatcg cacggctcgc caagcaattc gaggactcca ggcagcaact ggatcggcag
      241 atcgccaatg cgcagcaggc gttgaaaaaa atgcgggcgg aagatcaatc gctgacgcaa
      301 caggccagcg atttggccag cgcttgtcgg gccgaccatc tgctggcatg gctgcgcggc
      361 ggcgtgatcg gccgaggcga tgccatcgaa ctggccgaac cctggcatat cgagggctgg
      421 cgacaggccc gagcgcgcgt ctttattcag gcactgaagc tgcatcggac gttcttcgag
      481 ctggaggcgg cacggatgcg ctccaagcct tctttccgaa gttcggccag cacggcggcg
      541 gtggggatgt agctgtaccg ctcggaacgg ctctcatgcg gggcgtccgc gaagatggac
      601 ggggccacgc ggcggatttg gtcatcggat agcggggaat cgctgcgcag cgacggggaa
      661 cgggaagcga agcgggatgc gagttgcatg gtctttctcc tgacaaaaag ggttggctgt
      721 tcacaccgca caccggattc caagattcgg agcccagcct ttcggctgtt cggtgcggtc
      781 ggcacgagga acccggttgg cctcgttgcc accgtctttc ctgagttcat cgcccgcgac
      841 ggtcaggagc gcgcggacgg gggccgtcaa ggagacaagc gcagggttgg tgcggcccgc
      901 aggcgcggcc gaggacacgg ccctgcgcgc cttgacggca cacggacgcg ggctacagtc
      961 gcgagcaagg tgatggagtc aggggagatg gctggacaga caacggcctt ccctgtgtgc
     1021 tgaccgcacg gcaagcgaag cgcgcaggcc cggatttgga agccgggccg gaggcgtcag
     1081 ggatgtggaa ggctggcgaa tgccagtccc atcagggatg agcgaatagc gaacctggag
     1141 cagcgcggtc cgcgaagcgg agacgcgtga cggtgagatg tgagtagtat tgtttttctg
     1201 atagattgaa cggataaata ttgtccattc actcagcaga aatactatgc aaaacccaaa
     1261 taatgcgaca tttcactatt gctgcattta tgctgccaaa ggcattatcg acccgaatca
     1321 gaagctggat tcggcattac tgaaagacga gaatattgtg gttcgacttc taggatctga
     1381 tgcttatgaa aacctggttt ggcgaattgt ttcgccggca ggcaaaaaag ggattgaaga
     1441 tcttgatgac aaggccagag aaaaacttga acttcgccga aacagcttaa tccttcaagc
     1501 tctgcatcat gctgtgatcc atgtcgaatc atgtcttcct ctagatattg tccgcattta
     1561 tcaggaggaa aactccgagc taccagctta tgttaaagcc ccgctaaaga aaaatgcaga
     1621 acgtgctttt aaatctgttt cagcagcaat gacatttgcc agagggaatt ttctctcgaa
     1681 aaattatgat ttcatccatg agcaatacta cgttaccgac gatcaatcta cggtaattca
     1741 aaagctgcac cctgccacta tctcagatgg ggatttaaag acgaaactga gtatggaaga
     1801 ggtgcaggtt acagagaaat tagcatccat tctccatcat gacaaagaaa taaataagat
     1861 aattgatatc tactcagagt ctcttacgcc atacaagagc ggtcatcttc atgcctttat
     1921 tgccgcgtgg actgctcttg aaatgtttgt tgcaacacaa tttaaagaaa ttcaatcaag
     1981 cgtagccata aatatcaacg gaactgctgc acataatgat ttttcaagcc gcatgcttgc
     2041 cgtcatgaag gacaaatatc gcttggtaga taagtttgca gctttatcaa gctattatga
     2101 agatgcagat gctgatgagg gcatagcagt actcaagagc ataaaagaaa ttagagataa
     2161 gttcttccat acaatggaag gagaaataaa ggacttacca ttggatcaga ccagggaatt
     2221 gatctcgaag taccttcaaa tctatcttga aagaaaatgt gagggctttg tttttcaaaa
     2281 agctgctaga tagcactgcg ccacgcaagc agtcaggtgt cagcgacggc tgccaggacg
     2341 caagcac
//
LOCUS       DAOQEW010000373         2335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_25745_length_2335_cov_8.674123, whole genome shotgun sequence.
ACCESSION   DAOQEW010000373 DAOQEW010000000
VERSION     DAOQEW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_2335_cov_8.674123"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLS35_20185"
     CDS             complement(<1..350)
                     /locus_tag="PLS35_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNI21046.1"
                     /translation="MGRQNVSTGGPWEGKIGYSRAVRVGQSVQVSGTTAMTGAGLVGK
                     NDPYAQTIQALKTIEAALVQAGASISDVVRTRIYMANIDQWQEVGRAHGEVFGSVRPA
                     TTMVEVKRLIDPDML"
     gene            complement(528..1259)
                     /locus_tag="PLS35_20190"
     CDS             complement(528..1259)
                     /locus_tag="PLS35_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNI21047.1"
                     /translation="MRPLTGKVAIVTGSSSGIGRAIAERLARDGATVVVHYHVHAEKA
                     NQVAEGIQSSGGSALVRQADMSRVADAQRLVRETAKELGRLDILVNNAGRFLPRPLLE
                     TTEEEFDAILALNAKGPYFAMQAAARVLSDGGRIVNISSALTHLKFPGATAHLGSKAA
                     LEQYGKGLAQELASRGITVNTVLPGFTDTGVLTEPYRQMGAQMSPTQRLGHPSDIADV
                     VAFLVSEQARWLTGQVIHAGGGLVM"
     gene            complement(1535..2140)
                     /locus_tag="PLS35_20195"
     CDS             complement(1535..2140)
                     /locus_tag="PLS35_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNI21048.1"
                     /translation="MEKPAETQYPIHDVLRQRWSPRAYSERMVEPEKLRSLFEAARWA
                     PSSNNEQPWHFIVGTKADPAAYDRLFACLKEGNKKWAFRAPVLMLSVARLNFEDEGTP
                     NRHALHDTGMAALSLCLQATAMGLIAHQMAGFDMEKARTDLGLPAGHEPVAMIAVGYP
                     GDAAELPDYLREREFKPRERKLSTEFVSHGTWNDQPGWLKP"
     gene            complement(2159..2323)
                     /locus_tag="PLS35_20200"
     CDS             complement(2159..2323)
                     /locus_tag="PLS35_20200"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21049.1"
                     /translation="MGGGAVILPGVTIGDRSVVGAGSVVVHSVPADCVAVGNPARVVR
                     TLGSPTLTTE"
BASE COUNT          459 a          766 c          635 g          475 t
ORIGIN      
        1 agcatgtccg gatcgatcaa gcgcttcacc tccaccatgg tggtcgcagg cctgaccgac
       61 ccaaacacct ccccatgtgc gcggccgact tcctgccatt gatcgatatt ggccatataa
      121 atgcgcgtcc gcacgacatc agaaatcgat gcgccggcct gcacgagcgc cgcttcaatc
      181 gtcttcaagg cctgaatggt ctgcgcgtag ggatcattct tccccacgag tcctgctccc
      241 gtcatcgccg tggtgccgga gacctgcacg gattgaccga cccgcactgc ccgcgaatag
      301 ccgattttgc cttcccaagg tcccccagtc gaaacgttct gccttcccat gattcctcct
      361 ttgatcgtct gcgaacactc cactttacac ggcacgcagg atgcgcaaaa aggctttcca
      421 gcaaggcccc agtgaatgga gagccgaagc gtacccctta cggtacgttg aggttcgcca
      481 tgaagcgaga acgccgctgg aggctgtgtt ccccatcctg ctacacgtta catcaccagg
      541 ccgccgcctg catgaatcac ctggccggtc aaccatcggg cctgctcact cactagaaac
      601 gccaccacat cggcgatatc agaaggatgt ccgagccgct gcgtgggcga catctgcgct
      661 cccatctggc gatacggctc cgtcaagact ccggtgtccg taaaaccggg aagcacggtg
      721 ttgaccgtga tcccccggga ggccaactcc tgtgcgagcc ctttgccgta ctgttccagg
      781 gctgctttgc tccccaggtg cgcggtggcg cccggaaact tcagatgggt gagcgccgac
      841 gaaatattca cgatgcgtcc gccgtcgctg agaacccgcg ccgcggcctg catggcaaag
      901 tatggtccct tcgcattcaa agcgagaatc gcatcgaact cctcttcggt cgtctccagt
      961 aggggacgag gaagaaaccg gcccgcgttg ttcacgagaa tatccaggcg gcctaactcc
     1021 ttcgctgtct cccgcaccaa tcgctgggca tcggccaccc ggctcatgtc ggcctgccgc
     1081 accagggcgc tgcctccact ggactgaatc ccttcagcca cctggttggc cttctcggca
     1141 tgcacatggt agtggaccac aacggtcgcg ccatccctgg ccaatcgttc ggcaatcgcc
     1201 cggccaatgc cgctggaaga ccccgtgaca atggcgactt tgcccgtcaa aggcctcatt
     1261 ccggcacctc cgccttaacc atgcgttcca ttgatgatcc cgttcgcgca atcgttgtgg
     1321 attagaaaga ccgttgcatt gagcctggac ggtctgcggc agacagcccg gcgcctgtac
     1381 actgtatcaa ttcagctgag ccgagatcca acggcaattc ttacggtgcg caagatagga
     1441 agcaccagcg tgagaatact atgcgtagag gacgggcgag gatcggaccg ttgtgtctcg
     1501 ggtccgttca attgatagga aggagaggcg cctcctatgg tttcagccat ccaggctgat
     1561 cattccaggt accgtgagag acgaattccg tgctgagttt ccgttcgcgt ggtttgaatt
     1621 ctcgttcgcg tagatagtcc ggcagctcgg ccgcatcacc aggatatccg acggcgatca
     1681 tcgccaccgg ctcatgcccc gcaggcagac cgaggtccgt acgagccttc tccatgtcga
     1741 agcctgccat ctggtgcgcg atcagcccca tcgccgtggc ctgtaagcac aagctcagcg
     1801 ccgccattcc cgtatcatgc aaagcgtgcc ggtttggcgt gccttcatcc tcgaaattca
     1861 gccgcgccac ggagagcatc agcacgggcg cccggaacgc ccattttttg ttgccttcct
     1921 tgagacaggc aaacagtcgg tcatacgccg ccgggtcggc tttcgtgccg acgatgaaat
     1981 gccatggctg ttcgttgttc gaagagggag cccatctggc cgcttcaaac aggcttctca
     2041 acttctccgg ttcgaccatc cgttccgaat aggcccgcgg gctccagcgt tgccgcagca
     2101 catcatgaat gggatattgc gtctcagcgg gcttttccat gaacgatcct tccgtgaatt
     2161 attctgttgt cagggtgggt gagcccaatg tgcgcaccac gcgtgccgga ttgccgaccg
     2221 cgacacaatc ggcggggacc gagtgcacga ccacgctgcc cgcacccacc acgctgcgat
     2281 caccaatcgt cacaccgggc agaatcaccg ctccgccgcc gatccagacg tcatt
//
LOCUS       DAOQEW010000374         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_26829_length_2282_cov_9.527616, whole genome shotgun sequence.
ACCESSION   DAOQEW010000374 DAOQEW010000000
VERSION     DAOQEW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26829_length_2282_cov_9.527616"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLS35_20205"
     CDS             complement(<1..299)
                     /locus_tag="PLS35_20205"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21050.1"
                     /translation="MSARWPRQILFGDIDAMFASAAVLADPSLAGKPVAVGGPPPRGI
                     IAAASYAVRRFGVRSAMPTIQALRLCPQLVLVPPDRPLYTRLHRQLQEVTDRFFP"
     gene            complement(306..917)
                     /locus_tag="PLS35_20210"
     CDS             complement(306..917)
                     /locus_tag="PLS35_20210"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HNI21051.1"
                     /translation="MAPSVEPLLTLWTNLNRRYFQGALPQIPIEWSRRLTSSAGMFVC
                     RVGPRHTHMQSTIDQTRRRCIKLSSVLLPAHNLDLDRETMTTLAHEMIHQWQYDVLKR
                     RPNHGQDFRRMMARMNQDGLGITIYHSLGKAVTALAKYAWRCQQCGSMYRRQRRTIQP
                     RRHLCGACRGPLRECAVVPDEPLAEPIPVGPVGRQFSLPFQSS"
     gene            complement(974..1972)
                     /locus_tag="PLS35_20215"
     CDS             complement(974..1972)
                     /locus_tag="PLS35_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21052.1"
                     /translation="MPTRPKRTARSSTPPAIIKPIDASHLKTYPLQRRHSKVQVSNFA
                     KVWTGGRSFKRFLDSLPDILAVKTLREVAQAIAKAHRRGRPVIVGMGAHVVKVGLGPL
                     LVDLMERGIVTALAMNGAVIIHDFELAFMGHTSEEVDAEIDSGRFGMAEETGRMLNEA
                     ITLGMKDGLGLGESLGLYINRHKQQFPNRATSLLATGARLGLPVTVHVAVGTDIIHMH
                     PSADGAAIGAGSLLDFRRLAAVVSGMEGGVYLNLGSAVILPEVFLKAVSLGRNLGHSL
                     TNITTVNMDFLTHYRPMTNVVRRPTQKGGKGYALTGHHEIMVPLLAAAVIEELSGR"
     gene            complement(1956..>2282)
                     /locus_tag="PLS35_20220"
     CDS             complement(1956..>2282)
                     /locus_tag="PLS35_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNI21053.1"
                     /translation="AITCIANDYGFEELFARQVRAHGQAGDVAIAISTSGNSPNVLKG
                     VEAAKAIGLTTVAWTGGSGGKLAGMVDYGFVVPSTLTARIQESHITLGHVLCELIEDH
                     VLANPA"
BASE COUNT          443 a          671 c          748 g          420 t
ORIGIN      
        1 tcaggaaaga accggtccgt cacctcttgg agttgccgat ggaggcgtgt gtagagcgga
       61 cgatcaggcg ggaccaggac cagttgaggg cagaggcgca gggcctggat cgtgggcatg
      121 gccgatcgca caccgaatcg acggaccgca taactggcgg ccgcaataat accgcgcgga
      181 ggcggacctc ccacggcgac cggtttgccc gccaatgatg gatcggccag caccgcggct
      241 gacgcgaaca tggcgtcgat atcgccgaat agaatttgtc gaggccaacg agcggacatg
      301 aaaccctagc tcgattggaa tggaagactg aattgccggc cgacgggacc cacagggatt
      361 ggttctgcca gcggctcatc aggcacaacc gcacactcgc ggagtggtcc ccgacaggcg
      421 ccgcagaggt gccggcgcgg ttggatggtc cggcgctggc gccggtacat cgagccgcat
      481 tgttggcagc gccaggcata tttggccagc gccgtgaccg ctttgcccag ggaatggtag
      541 atcgtgatgc cgaggccatc ctggttcatg cgcgccatca tgcggcgaaa gtcctgacca
      601 tgattcggac gacgcttgag cacatcgtat tgccactgat ggatcatttc atgcgccagc
      661 gtcgtcatcg tttcgcgatc caggtcaagg ttgtgagctg gcaggaggac cgacgacagc
      721 ttgatgcagc ggcgtcgcgt ctgatcgatg gtgctctgca tgtgcgtatg tctcgggccg
      781 acccggcaca cgaacatgcc cgcagaggaa gtcagccgcc ggctccattc gatgggaatc
      841 tgcggtaagg cgccctgaaa atatcgacgg ttgaggttgg tccacagggt gaggagcggt
      901 tcgacagagg gtgccatgtt gatcacagaa cgatacgagg ccgtgcgagg tgaacgcgta
      961 cgagttccgg attttaccgg ccggacaatt cttcgatgac ggcggcggcc aacagcggca
     1021 ccatgatttc gtgatgaccg gtgagggcgt agcctttccc gcctttctga gtcgggcggc
     1081 gcacgacgtt cgtcatggga cgataatggg tgaggaaatc catgttgacg gtcgtgatgt
     1141 tcgtcagcga gtggccgaga ttccgtccga gcgacacggc tttcagaaac acttccggca
     1201 ggatgacggc agatcccagg ttcaggtaga ccccgccttc catcccggag accacggcgg
     1261 ccaagcggcg gaagtcgagc agtgacccgg cgccgatcgc cgcgccgtcg gcggacggat
     1321 gcatatggat gatgtcggtg ccgacggcca cgtgcaccgt gacggggagg cccagtctgg
     1381 ctccggtcgc caagaggctg gtggcccggt tcgggaactg ttgcttgtga cgattgatat
     1441 agaggcccag tgattccccg aggcccaggc catctttcat gcctagcgtg atggcttcgt
     1501 tgagcatgcg gccggtttct tccgccatgc cgaatcgacc ggaatcgatc tccgcgtcga
     1561 cttcctcaga agtgtgcccc atgaaggcca gctcgaagtc gtggatgatg acggctccgt
     1621 tcatcgccag cgcagtcacg atgccgcgct ccatcagatc cacgagcagc gggcccagtc
     1681 cgaccttcac cacgtgcgcg cccatgccga caatcaccgg ccggcctcga cgatgtgcct
     1741 tggcgatggc ctgcgccacc tcccgcagcg tcttgaccgc caggatatcg ggcagcgaat
     1801 cgagaaagcg tttgaacgag cggccgccgg tccagacctt ggcgaagttc gagacctgca
     1861 ccttgctatg ccgccgctgg agcggatacg tcttcaagtg agacgcatca atcggcttga
     1921 tgatcgcagg gggcgtcgat gatcgcgcgg tgcgcttagg ccgggttggc aagaacgtga
     1981 tcctcaatga gttcgcagag cacatggccc agcgtgatgt gactttcctg aatgcgggct
     2041 gtcaacgtcg aagggacgac aaagccgtag tcgaccatcc cggccaactt gccgcctgag
     2101 ccgccggtcc aggccacggt cgtgaggccg atggccttag ccgcttcgac acctttcagc
     2161 acattagggg agttgccgct ggtgctgatg gcaatagcca catcgcccgc ctgcccgtgc
     2221 gcgcgcacct gacgggcgaa gagttcttcg aagccgtagt cgttggcgat gcaggtaatc
     2281 gc
//
LOCUS       DAOQEW010000375         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_27176_length_2266_cov_8.256897, whole genome shotgun sequence.
ACCESSION   DAOQEW010000375 DAOQEW010000000
VERSION     DAOQEW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27176_length_2266_cov_8.256897"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..706)
                     /gene="modB"
                     /locus_tag="PLS35_20225"
     CDS             complement(32..706)
                     /gene="modB"
                     /locus_tag="PLS35_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNI21054.1"
                     /translation="MNWIAIWVTCKLAGLTALALLFVGLPIAYWLSFSRWRWKFLIES
                     VVALPLVLPPTVLGFYILIAIGPHSPFGRLYTELVGHPLPFTFEGLLLASILYSLPFA
                     VQPFAAAFDQVDRRLIEASWTLGVSKLQTFFRLIMPLSTAGLVTGAVLSFAHTMGEFG
                     VVLMIGGNLEGSTRTVSIDIYDEVQALNYAGAAKTALFLLVVSYAVLLVVYAVNRNVW
                     AAWPRK"
     gene            complement(790..993)
                     /locus_tag="PLS35_20230"
     CDS             complement(790..993)
                     /locus_tag="PLS35_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNI21055.1"
                     /translation="MKLSARNQFQGNVVRINEGQAMAEVVVKVGTLEFVAAITEGSVK
                     NMGLKVNDQVTVAVKATEVMIGK"
     gene            complement(1007..1384)
                     /locus_tag="PLS35_20235"
     CDS             complement(1007..1384)
                     /locus_tag="PLS35_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21056.1"
                     /translation="MVSHYQSKHWWLVLCGALFVTAGCSQAVLMEQESAQGGVVTYSY
                     KQDRGGPMGSPYRKQALDLMQAKCGGSSRLLREGEVRGYTSAGMGTIEGTEDEERGRR
                     WGIRFECKDREGGDKPAESLQHR"
     gene            complement(1362..2120)
                     /gene="modA"
                     /locus_tag="PLS35_20240"
     CDS             complement(1362..2120)
                     /gene="modA"
                     /locus_tag="PLS35_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI21057.1"
                     /translation="MRRFFAIILLILCAVSPVRAEELAVAAASDLNFAIKELIGEFEK
                     QTGHRVKVSLGSSGNFYAQLQQGAPFDLYFSADIGYPKKLEEGGLTVPGSLYRYAVGR
                     VVLWAPKSSPLDVNKGLTVLREPTIRKIAIANPKHAPYGRAAVAAMEHAQVYADVKDR
                     LVLGENISQAAQFIESGACDVGIIALSLALAPAMKSAGSYWEIPAEAHPPLEQGAVIL
                     KQSKNQELARSFLSFLQGPQGQEIMTRYGFTLPK"
BASE COUNT          528 a          748 c          559 g          431 t
ORIGIN      
        1 cgaccgggaa acgtcttcac gagatggatc gttatttccg cggccatgct gcccacacgt
       61 ttcgattgac ggcatacacc accagcaaca cggcatagga caccacgaga aggaacaggg
      121 ccgttttggc cgcgccggca taattcaacg cctgaacctc atcatagatg tcgatggaga
      181 ccgtgcgggt ggaaccttcg aggttgccgc cgatcatcaa caccacgccg aactcgccca
      241 tcgtatgggc aaaactcaac acggcgccgg tcaccaagcc ggctgtcgat agcggcatga
      301 tcagcctaaa aaaggtctgc agtttcgaca cccccaaggt ccaggacgct tcgatcaacc
      361 gccggtccac ctgatcgaac gccgccgcaa acggctgcac cgcaaacggc agactataga
      421 ggatcgaggc gaggagtagt ccttcgaacg tgaagggcag cggatgaccg accaactcgg
      481 tatacagacg cccgaacggg ctgtgaggac cgatggcgat aagaatatag aaaccgagaa
      541 cggttggagg gagcacgagg ggcaacgcga ccacggactc gatcaaaaat ttccaccgcc
      601 aacgcgagaa actcagccaa tacgcgatgg gaaggccgac aaagaggagc gccaacgccg
      661 tgaggccggc taacttacac gttacccaaa ttgcgatcca gttcacgcgc gcgccccagc
      721 cacgaccaga cgttcccgac cggcgccatc gtaagaacac cggccgggaa gatcaactgc
      781 ctccgccgat tattttccga tcatcacctc ggtggccttc accgcgacgg tgacttgatc
      841 gttgaccttg agacccatgt tcttcaccga cccttcggta atggcggcca caaactccag
      901 cgtgcccacc ttcaccacta cctcagccat cgcctgccct tcattgatac ggaccacgtt
      961 cccctgaaat tgatttcttg cgctcagctt catgttcgac tcccttctat ctgtgctgaa
     1021 gtgattcagc tggtttgtcg cccccttcac ggtccttaca ctcaaaccgg atcccccacc
     1081 gtctgccgcg ttcctcatct tcggtacctt cgatcgtacc catgcccgcg ctggtgtagc
     1141 cgcggacctc gccttctctc agcaaacgtg aactgccccc gcacttggcc tgcatcaaat
     1201 ccaacgcctg cttccgatag ggcgaaccca tcggccctcc ccgatcctgc ttatagctgt
     1261 acgtcaccac tcctccctgc gcggactcct gctccatgag caccgcctgc gaacagccgg
     1321 cagtcacgaa cagagcgccg cacagcacca accaccagtg tttactttgg taatgtgaaa
     1381 ccataccggg tcatgatctc ctggccttga ggcccttgca gaaaactcaa aaagcttcgg
     1441 gcgagttcct gattcttcga ctgcttcaga atgaccgccc cctgctccag tggcggatgc
     1501 gcctccgcag gaatttccca atagctgccg gcactcttca tggctggggc cagcgccagc
     1561 gaaagcgcga taatccccac atcgcaggca cccgattcaa tgaactgtgc cgcctgagag
     1621 atattctcgc ccagcaccaa tcgatccttc acatcggcat agacttgggc atgctccatc
     1681 gcggccacgg ccgcacgccc gtaaggtgcg tgcttgggat tcgcgatggc aatcttcctg
     1741 atcgtaggtt cccgcaggac tgtcagtccc ttgttcacgt ctaaaggaga ggacttcggc
     1801 gcccacagca ccacgcgtcc caccgcatac cgatacaacg aaccaggcac ggtcagcccc
     1861 ccttcctcca attttttcgg atacccgata tccgctgaaa aatagagatc gaagggggct
     1921 ccctgctgga gttgtgcgta aaagttgcct gacgacccca gcgacacctt cacgcgatga
     1981 ccggtttgct tttcaaattc gccgatcaat tccttgatcg caaagttgag atcagacgcg
     2041 gcagcgaccg ccagctcctc ggctcggaca ggactcaccg cacacaagat gagcaggatg
     2101 atcgcgaaaa atcttctcat attcagtact ccctagaaag ttgtggacac acttgcttgt
     2161 cacggcataa ctctcactcg tatccacacg ccgtcccgtc aacagacacc acaggatgcg
     2221 caaaaaggcc gtccagcaag gccgcagcaa gcgaagaggc gaatcg
//
LOCUS       DAOQEW010000376         2232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_27974_length_2232_cov_7.654571, whole genome shotgun sequence.
ACCESSION   DAOQEW010000376 DAOQEW010000000
VERSION     DAOQEW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27974_length_2232_cov_7.654571"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PLS35_20245"
     CDS             complement(<1..1553)
                     /locus_tag="PLS35_20245"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HNI21058.1"
                     /translation="MGLLLWFVFGGVAVASSNLPLHHWGYDAIERLIALGVIDRALVG
                     AKPFTRMQAAQYVARAIERVRADEIDIDGREAIAEPLLERLLAEFRPELIRLGTIKAR
                     PEDKTGALRFGARVTSEVDASSIGGGQTIRFRENRGGEYFVNGVQNQTDVRAWAELNE
                     WAAVMVQPKFISNRHLLGLGATNNSDNFYLREFSLKLSYANIALEAGRGTQWWGPGYH
                     GSLLLTDHAFPMDMIKLGTERPFQLPGFLSGLGDWKVNAFLAQLERNRDFSRARVFGL
                     RISYLPVSWLEFGLTRLTQFGGRGRDQSFPETIVDTYIHPANQGSDKDVNEQGMADFR
                     LRLPSVPYLIPFPSGLQLYGEIGTEDKWSQLPIPSRAAFLGGIYIPQVFQGDTMDLRI
                     EYADTDYGRRRHPELGGVWYNNGTYTSGMRYRGFPLGHHMGTDGTDFFVRSTRYLTDT
                     LQLGANFNLQERDRGQPVHEKKREAAVDLTWWYSKDIQVMGGYTFQRLTNPGQVSAIT
                     PFAETFAAGV"
     gene            complement(1882..>2232)
                     /locus_tag="PLS35_20250"
     CDS             complement(1882..>2232)
                     /locus_tag="PLS35_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21059.1"
                     /translation="EFNPNAQGRRYYCARELSALLREAGFQPDLFGAFPVGSTSISAR
                     CLSWIKRAAVQLRLIPSSMAGKRILKRLFLGELVPFPAEVDDEMAGYVPPVSVSPEHP
                     VTGYKILFAVCQVA"
BASE COUNT          542 a          665 c          603 g          422 t
ORIGIN      
        1 acgccggcgg cgaaggtttc agcaaacggc gtaatggccg acacctgtcc cggattcgtc
       61 aaacgctgaa aggtgtaccc ccccatgacc tgaatgtctt tggaatacca ccaggtgaga
      121 tcgacggcag cttcacgttt tttctcatga accggctggc cgcgatctcg ctcctgaagg
      181 ttgaagttcg cgcccagctg cagagtgtcg gtcaggtatc tggtgctccg cacaaaaaag
      241 tccgtcccgt ctgttcccat atgatgcccc aacggaaacc cccggtagcg catgccactg
      301 gtataggttc cgttgttgta ccacacgccc cccagttcag gatgccgccg ccggccgtag
      361 tccgtatcgg catattcgat gcgcaggtcc atggtgtcgc cttgaaacac ctgcgggata
      421 tagatgccgc ctaaaaacgc cgcacggctc gggatcggca gttgcgacca cttgtcctcc
      481 gtcccgatct cgccatagag ctggagacct gaaggaaagg gaatcaggta aggaactgat
      541 ggcagcctca gccggaaatc ggccatgccc tgttcattca cgtccttgtc agacccctga
      601 ttcgccggat ggatgtacgt atcgacgatc gtttcgggaa acgattggtc gcgtccgcgc
      661 ccaccgaact gcgtcaagcg cgtgagcccg aactccaacc aggagaccgg cagataactg
      721 atgcgtaatc cgaaaactct cgcacgtgaa aaatcgcgat tgcgttccag ctgagccaga
      781 aaggcattga ccttccagtc acccaggccg ctcagaaacc ctggcaattg gaacggccgc
      841 tccgtgccca atttgatcat atccatggga aacgcatgat ccgtgagcaa cagagagccg
      901 tggtagcctg gcccccacca ctgtgttcct ctccctgctt cgagcgcaat gttcgcataa
      961 ctcaacttga ggctgaactc gcgcagataa aagttgtcgc tattgttggt cgcgcccaat
     1021 cccaagagat ggcgattgct gatgaatttc ggttgaacca tcacggcggc ccattcgttc
     1081 aactccgccc aggcgcgcac atcggtttgg ttctggacac cattgacgaa atattccccg
     1141 ccccggtttt cccgaaaccg gatcgtttgc ccgcctccaa tcgatgaggc atcgacctca
     1201 gaggtcacgc gggcaccgaa ccggagagca ccggttttgt cctcaggacg tgccttaatg
     1261 gtgccgaggc gaatgagttc cggacggaac tccgccagta atcgttccaa cagcggctcg
     1321 gcaatagcct cgcgaccgtc aatgtcgatt tcgtcggcac gcactcgctc tatagcgcgt
     1381 gccacatact gcgccgcctg catgcgggta aacggcttcg cgcccaccag ggcccgatca
     1441 atcacgccca gagcaatgag acgttcaatc gcgtcatatc cccagtggtg caatggaaga
     1501 ttgctcgagg caacagcaac gcccccgaac acgaaccaaa ggaggagtcc caccgtcagg
     1561 aagcgtagga agggccggac tttcacaggg caggccgaat cacaacaata gaaagagacg
     1621 cttgagcgac gcaaggctgc aagaccaact cattggaaaa ggccgtatgg cccagacagg
     1681 caccgaaagg cttgagacgc cgatgaacgg tcggcctggc cgtgggatcc aaagtcggca
     1741 tgaccaatct atggagagac agtttcttga atctcggaat tgccaaactg ggaagatact
     1801 ctgtctcgaa caaccgaatc cagtcctcgg cgatactcag tcacaagtgc tgtcccaatt
     1861 gagcaaacgg tagggcgaag gtcaggccac ttggcagaca gcaaagagga ttttgtaacc
     1921 ggtcactgga tgttcgggag aaacggaaac gggaggaacg tatccggcca tctcatcatc
     1981 cacctcagca ggaaagggaa caagttctcc cagaaacagc cgtttcaaaa tacgcttccc
     2041 cgccatactc gatggaatca gacgtaattg aacagcggca cgcttgatcc acgacagaca
     2101 gcgcgcagaa atcgacgtcg acccaacagg gaaggcgcca aagaggtccg gctggaaccc
     2161 ggcctctcgt agcaacgccg aaagttcccg ggcgcaatag tatcggcgac cctgagcgtt
     2221 cggattaaat tc
//
LOCUS       DAOQEW010000377         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_28179_length_2223_cov_8.907749, whole genome shotgun sequence.
ACCESSION   DAOQEW010000377 DAOQEW010000000
VERSION     DAOQEW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28179_length_2223_cov_8.907749"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(465..1412)
                     /locus_tag="PLS35_20255"
     CDS             complement(465..1412)
                     /locus_tag="PLS35_20255"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNI21060.1"
                     /translation="MESVGEFFRQVRETKGLTVDEVASKTRIRTDFVKALEEGNFAKL
                     PDQVFARGFVRSYARSLGLDEEDAIHRFVQSAGAFYEKQGERERLRQRQVEEERRRRA
                     NRKAVGVAIAVAIATLVFLLSREQTSTLVRRSGPDAPTVSKKVAPFAKDAKEAGSRPA
                     AELPPPIAPPLKPAEPAVVPAKPAQDKPAPVAQAPAPVAVAKAPLPSDTVVTQPSASM
                     GSDGPLAGLSVDGPVGSDGPLVLDLAATELSWVVVQVDSGSHQEALLRPGEKAQWKAQ
                     DQFTVTLGNAGGVRAELNGKPQKPFGPSGKVVRDVVIKR"
     gene            complement(1422..2120)
                     /locus_tag="PLS35_20260"
     CDS             complement(1422..2120)
                     /locus_tag="PLS35_20260"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNI21061.1"
                     /translation="MIVVDRQETRSCRASGMVGSSCLVFLLGLSVLGGCANEENLRKS
                     KGFYQEGVARLSSDQQQAYVSFQKAVKLNPDNKEAHYGLGHIYASQGRLKLAEESFRE
                     AIRIDGDYAEANTYLGQVLASQDRWKDAIAAYRQALSNPLYPTPDLARFQLGKALMHE
                     GDLQGAMEVLEDATTVTPPNVPPAMLQLELARVYHKLGFDARAREALSKVSTLDKGGE
                     QAAAAQELLGKLKP"
BASE COUNT          473 a          713 c          590 g          447 t
ORIGIN      
        1 tagaaaaata ccccatcaga actcctcctt tgtgttgaac ccaccgcgtg aacaataact
       61 ctcggtccga atcagcatgg caaaaactga tgacaaagcc gataaagcag gcgaactata
      121 gcaacgcgtt ttctgtcttg tcaagcgaat ggcccgcctc agccttgtct gtttcatcca
      181 tgctgacttg aaggagaatc aagggctgtg ccagcggcag aacagcaaaa atcgcgggat
      241 ttcaccacat ttagactatt cagatcagcg gcaagtacgt acagccatcc agccgtgaca
      301 cctttgcgcc tcagacgcct cctcctcgcc tcattcgagt gcgagcagga gggccataga
      361 caccctcacg acatatggat tggagagagt gagcaggcgg tgagaatagg aatgcacgaa
      421 acgcctgacg acccggggag cgtcgagcta gtgagacgac accgtcaccg tttgatcacg
      481 acgtcccgga caaccttgcc gctggggccg aacggctttt gcggtttgcc gttgagttcg
      541 gcgcggacgc ctcccgcatt gccgagcgtc accgtaaact gatcctgggc cttccactga
      601 gccttttcac ccgggcgcag cagcgcttcc tggtggctcc ccgaatcaac ttggacgacc
      661 acccagctca attccgtagc cgcaaggtcg aggacgagcg gaccatccga cccgacggga
      721 ccatccaccg acaatcctgc cagaggaccg tctgatccca ttgaggcaga gggctgagtc
      781 acgaccgtat ccgacggcaa cggcgctttg gccacggcaa cgggcgctgg agcttgcgca
      841 accggagccg gtttgtcttg ggcaggcttc gcaggaacaa ccgccggctc agccggtttg
      901 agcggaggag caatgggagg cggcagctcc gccgcagggc gggaacccgc ctccttcgca
      961 tccttggcaa atggggccac cttcttactg acggttggtg catccggtcc ggagcgtctc
     1021 accagcgtag aggtctgctc acggctcaac aggaacacca gcgtcgcaat cgcgacagcg
     1081 atggccaccc ccaccgcctt gcggttggca cggcgacggc gctcctcttc gacctgtcgt
     1141 tgccgcaagc gttcgcgctc accctgcttt tcgtaaaagg cgccggcaga ctgcacgaat
     1201 cgatgaatgg catcttcctc atccaatccg agcgaccgcg cataagaccg gacaaatccg
     1261 cgcgcaaaaa cctggtctgg caatttggcg aaatttccct cttcgagcgc tttgacaaag
     1321 tctgtgcgga ttcgggtctt cgaggccacc tcatccaccg tcagcccttt ggtctcgcga
     1381 acctgccgaa aaaattcacc cactgattcc atgatatccg cctatggttt gagcttaccg
     1441 aggagttcct gcgccgcagc cgcctgctcg ccacctttat ccaacgtcga cactttcgac
     1501 agagcctcac gcgcccgcgc gtcgaatcca agcttgtggt acacacgcgc cagctccaac
     1561 tgcagcatcg cgggcggcac attgggcggc gtcacggtcg tcgcatcctc caacacctcc
     1621 atcgcgccct gcagatcacc ttcatgcatc aaggctttcc ccagctgaaa ccgcgccagg
     1681 tcaggtgtgg gatagagcgg attgctgagc gcctgccggt acgccgcgat ggcatctttc
     1741 catcgatctt gactggccaa gacctgcccg aggtacgtgt tggcttcggc atagtcgccg
     1801 tcgatccgaa tcgcctcacg gaaggattct tcagccagct tgagtcgtcc ttgcgaggca
     1861 tagatgtgcc cgagtccgta gtgcgcttcc ttattgtcag gattcagctt caccgctttt
     1921 tgaaacgaca catacgcctg ctgctgatca gaactcagcc gggccacgcc ctcctggtaa
     1981 aatcctttgg acttgcgcag gttctcttcg ttggcacacc cgcccaagac agacagcccc
     2041 agcaggaaca cgaggcagga tgaccccacc ataccgctgg ccctgcagct ccgagtttcc
     2101 tgtcgatcaa ccacgatcat gcgccgatat cctcaaaatg cgtgagccgc ttgaattctt
     2161 taaagcgcgc ctcaatctct ttgtaatcta ggatcctcaa tcggtcaagg ctaaaggctt
     2221 cta
//
LOCUS       DAOQEW010000378         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_28249_length_2220_cov_9.630947, whole genome shotgun sequence.
ACCESSION   DAOQEW010000378 DAOQEW010000000
VERSION     DAOQEW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28249_length_2220_cov_9.630947"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLS35_20265"
     CDS             complement(<1..293)
                     /locus_tag="PLS35_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21062.1"
                     /translation="MRLLLVMIALILCLTGLTHPGTSSAAEKSYYSPIINVDVDNSRI
                     LISTLGAVFWVDVPEEAKAHIEKLPQSGLVDIVVETREGQPPLLKTWKVKSGES"
     gene            complement(392..904)
                     /locus_tag="PLS35_20270"
     CDS             complement(392..904)
                     /locus_tag="PLS35_20270"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNI21063.1"
                     /translation="MPDLSIPQHILDQIVAHARELAPFECCGLLAGKNKVVTHLYRIT
                     NIVAVEGAEKLSTFDEDKVAHLERLSPEERAEIAFVMDMGDFSAAKKDIRKNGLDLQV
                     VYHSHPKDPARPSHTDIKIATDYEEIWQRINLPVPAYLLVSLMHAPAADIRTYWITGG
                     QVRPADIHIM"
     gene            complement(906..1709)
                     /gene="moeB"
                     /locus_tag="PLS35_20275"
     CDS             complement(906..1709)
                     /gene="moeB"
                     /locus_tag="PLS35_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNI21064.1"
                     /translation="MEFTDQQIQRYSRHIILNEVGGKGQVKLAKAKVLLIGAGGLGSP
                     AALYLAAAGIGTIGLVDGDVVDLSNLQRQILHTTATVGVPKVESGRRMLSAINPDITI
                     KTYQLNVDTENILGLVSDYDIVLDGSDNFTTRFLVNDGCFFAKKTLISASMFRFEGQL
                     TSIKPHAGFPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTMGILQASEAIKEILGIGE
                     TIADKLVIYDALEMKFRKVSRPKDPRCPLCSATPTIKDLGGDYSVACTI"
     gene            complement(1785..2024)
                     /locus_tag="PLS35_20280"
     CDS             complement(1785..2024)
                     /locus_tag="PLS35_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNI21065.1"
                     /translation="MTSLRFHIRFPENKIKEPIIYQIGHEFRVVTNVRRADVRETTGW
                     MDLELTGETEEIERAIDGIRTKGCVVDPIELNVVE"
     gene            complement(2027..>2220)
                     /locus_tag="PLS35_20285"
     CDS             complement(2027..>2220)
                     /locus_tag="PLS35_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNI21066.1"
                     /translation="VDMIGSLDATYPGLKNRLCDEKGDLRRFVNIYVNEEDIRFLNGK
                     ETSLKDGDEVSIVPAIAGG"
BASE COUNT          460 a          609 c          634 g          517 t
ORIGIN      
        1 gattcgccgg atttcacctt ccaggttttc aggagcgggg gctggccttc ccgcgtctcc
       61 acgacgatat ccaccaatcc tgattgcggt agtttctcga tgtgagcttt ggcttcctcc
      121 ggcacgtcca cccagaacac tgcgcccaaa gtcgagatca gaatgcggct gttatcaaca
      181 tccacattga tgatggggct gtagtagctc ttttcagctg ccgaggacgt cccgggatgt
      241 gtgaggccgg tgaggcacag gatgagggcg atcataacga gcaataatcg catcgaatac
      301 tcccttcgat gacaaggtca aagccggatt atggcatcgt tgctgagcga ttggtagggg
      361 gaacctggtc gaactgcagg cggggagacc gtcacatgat atggatatcg gcgggccgga
      421 cttggccgcc ggtgatccaa taggtgcgaa tgtctgctgc gggggcatgc atgagtgaaa
      481 ccagcagata ggccggaaca ggcaagttga tccgttgcca gatttcctcg tagtccgtcg
      541 cgattttgat gtcggtgtga gacggccgcg ccggatcctt cggatgggaa tgatagacca
      601 cttgcaggtc cagaccgttc ttgcgaatgt cttttttagc cgcggaaaag tcgcccatat
      661 ccatcacaaa ggcaatctcc gcccgttctt ccggagagag gcgctccaga tgcgcgacct
      721 tgtcttcatc gaacgtagaa agtttttccg ccccctcgac agccacgata ttcgtgatgc
      781 ggtaaaggtg cgtgacgact ttgttctttc cggctaacag tccgcaacat tcgaacggag
      841 ccagttcgcg cgcatgcgcg acgatttgat ccagaatgtg ctgggggatc gacagatcag
      901 gcatgtcaga tggtgcaggc cacgctgtag tctccgccca gatccttgat cgtcggcgtc
      961 gcgctgcaca aagggcaacg cggatctttc gggcgagaga ccttgcggaa tttcatttcc
     1021 aaggcatcgt agatcaccag cttatccgcg atcgtttccc cgatgccgag aatttccttg
     1081 atggcttctg atgcctgcag aatgcccatg gtaccggcca gcacacccag cacgccggct
     1141 tcctggcagt tcggaaccaa tccagcggga ggcggctcgg gatagaggca gcgataacag
     1201 gggaacccgg catgaggctt aatgctcgtg agctggcctt caaagcggaa catgctggcg
     1261 gagatcagcg tcttcttggc gaaaaagcat ccatcgttca ccagaaaccg cgtggtgaaa
     1321 ttatcggacc catccaagac gatgtcataa tccgacacca acccgagaat attctccgta
     1381 tcgacgttca gttgataggt cttgatcgta atgtcgggat tgatggctga cagcatccgt
     1441 cggcccgatt ccaccttggg cacgccgacg gtcgcggtgg tatgcaggat ctgccgctga
     1501 agattggaca ggtcgacgac atcgccatcg accagaccga tcgtgccgat gcctgccgcc
     1561 gccaggtaca gggcggcggg ggaaccgagt ccgccggcac cgatcaatag caccttggct
     1621 tttgccagtt tgacctgccc tttgccgccc acttcgttga ggataatgtg ccggctgtag
     1681 cgctgtattt gttggtcggt aaattccatt cgactcgtct cttgtcgttc gtcagaggtg
     1741 aatccgccct ctgaggtatg gtctgaaaga aggctgtagc ctggttattc caccacgttc
     1801 agctcaatcg ggtcgacgac gcagcctttg gtgcggatgc cgtcgatggc ccgttcgatc
     1861 tcttcggttt cgccggtgag ttcgaggtcc atccatccgg tggtctcgcg cacatcggcg
     1921 cgtcgcacat tggtgaccac tctgaattca tgcccgatct gatagatgat cggttccttg
     1981 attttattct cgggaaaacg aatgtgaaag cgtaggctgg tcatggttat cctccggcga
     2041 tcgcgggaac gatcgatact tcatcgccgt ctttcagcga ggtctctttc ccgttcagga
     2101 agcggatatc ttcctcattg acgtagatat tcacgaagcg ccgcaaatca cctttttcat
     2161 cgcacagccg gttcttcagg cccggatagg tggcatcgag cgatccgatc atgtccacaa
//
LOCUS       DAOQEW010000379         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_28580_length_2206_cov_7.436541, whole genome shotgun sequence.
ACCESSION   DAOQEW010000379 DAOQEW010000000
VERSION     DAOQEW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28580_length_2206_cov_7.436541"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1012)
                     /locus_tag="PLS35_20290"
     CDS             complement(89..1012)
                     /locus_tag="PLS35_20290"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNI21067.1"
                     /translation="MSNRVQEILSWYESDNAGTKTNIARLLNSGKLAGTGKLVILPVD
                     QGFEHGPARSFAPNPGGYDPHYHFQLAIDAGCNAYAAPLGFIEAGASHFAGRIPLILK
                     LNNHDVLHDEKDPLPSVTGSVQEALRLGCSAVGFTIYPGSSHCNPMYEQLRAIAEEAK
                     NAGLAVVVWSYPRGSGLSKEGETALDVVAYAAQIAAQLGAHIIKVKLPSAHLEQAAAK
                     KVYEAEKIPTATLTERVKHIVQSSFNGRRIVIFSGGAKSDEKTVFEEVRAIRDGGGFG
                     SIIGRNSFQRPKAEAVKFLNTIMSLYAGEKP"
     gene            complement(1049..2041)
                     /gene="fbp"
                     /locus_tag="PLS35_20295"
     CDS             complement(1049..2041)
                     /gene="fbp"
                     /locus_tag="PLS35_20295"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNI21068.1"
                     /translation="MAKFPITLSRFIIEQQAAHPEATGEFSVLLTQIGLVGKMIAHDL
                     RRAGLNKILGTTGETNVQGEIVKKLDQIANDTFVRVFEHSGLVCVLASEEMEKPLKMV
                     HEGARYMLLVDPLDGSSNTDVNMPLGAIFSIRRSRTGQSVEDELLQKGTEQIAAGYVL
                     YGASTMLVFTVGQGVHGFTLEPSIGEYLLSNENIRIPSKGKVYSVNEGNYHRWPAGTQ
                     RYLDYLKVQDKPSGRPYSGRYSGCLVADVHRILLGGGIYLYPGEKDKPEGKLRLMYEG
                     NPLAMVVEQAGGKALTGTMRILDVEPKALHQRVPLIIGSAEDVSLAEDYVQGRA"
BASE COUNT          468 a          682 c          573 g          483 t
ORIGIN      
        1 aatgctggaa agatccagga tcgcatcgac tgtcgctgtt caggtggaat cggaccgtca
       61 tccatagtgg ttcgtcagtc tggaccgact agggcttctc gcccgcgtac agactcatga
      121 tcgtgttcaa aaatttcacg gcttccgcct tcggccgctg gaatgaattc cggccgatga
      181 tcgacccgaa tcctccgcca tcacggatcg cccgcacttc ttcgaagacc gttttttcgt
      241 cgctcttcgc accgccggag aaaatgacga tgcgacgccc gttgaacgaa ctctgcacga
      301 tatgctttac gcgctccgtc agcgtcgccg tgggaatctt ttcggcctca tacaccttct
      361 tcgccgctgc ctgctccaaa tgggctgagg gcaatttgac cttgatgata tgcgcgccga
      421 gttgcgcggc aatctgcgcg gcataggcca ctacatcgag ggccgtctca ccttccttgc
      481 tcagccctga cccgcgcggg tacgaccaga ccaccacagc cagcccggca tttttggctt
      541 cctccgcaat cgcgcgcagc tgctcataca tgggattgca gtgagaagag ccgggataaa
      601 tcgtgaaccc cacggctgaa catcccagcc gcaaggcctc ctgcacgctg cctgtcaccg
      661 agggcagcgg atccttctca tcgtgcagca catcatgatt attcaatttg agaatgagtg
      721 gaattctgcc ggcgaagtgg ctggcaccgg cctcgataaa tccgagcggc gccgcgtagg
      781 cgttgcatcc ggcgtcgatg gccagttgaa agtggtaatg gggatcatag ccgcccggat
      841 tgggcgcaaa acttctcgcc ggaccatgct caaacccttg atcgaccggc aggatcacca
      901 actttccggt tccggcgagt ttccccgaat tcagcaaccg ggcgatgttc gtctttgttc
      961 ccgcgttgtc actctcatac cagctcagaa tttcctggac ccgattgctc ataatcaacc
     1021 tcccaaccaa cctcgatcac attagtcgtt acgcacgtcc ttgcacatag tcttccgcca
     1081 gactgacatc ctcagcactg ccgataatga ggggaacccg ttgatgcagc gccttgggct
     1141 cgacatcgag aatgcgcatc gtcccggtca acgctttgcc tccggcctgt tcgaccacca
     1201 tggccaacgg attgccttcg tacatcaatc gcagttttcc ctcaggctta tccttctctc
     1261 ccggatacag atagatgccg ccgcccagga gaatccgatg cacatccgcc accaaacacc
     1321 cgctgtatcg cccgctatag gggcgcccgg acggcttatc ctgcactttc agataatcga
     1381 gataccgctg tgtgccggca ggccagcgat gataattccc ctcgttcact gaatagacct
     1441 tgcccttcga aggaatgcgg atgttctcat tggacaggag atactcaccg atggacggtt
     1501 cgagcgtaaa gccatggaca ccctgcccga ccgtgaacac gagcatcgtg ctggccccat
     1561 acagcacata ccccgcggcg atctgttctg ttcccttttg caacaactca tcttcgacgg
     1621 attgtcccgt ccgcgatcgg cggatggaaa aaatcgcccc gagcggcata ttgacatcgg
     1681 tattggagga gccgtccaag ggatcgacca ataacatgta cctcgcgcct tcgtggacca
     1741 tcttcagcgg cttttccatc tcttcggagg ccaggacgca gacgaggccg ctgtgttcaa
     1801 acacgcgaac aaaggtatcg ttggcgatct gatccagctt cttgacgatc tcgccttgga
     1861 cattggtctc accggtcgtg cccaggatct tgttcaatcc ggcacgacgc aagtcatgag
     1921 cgatcatttt accgacgaga ccgatttgag tcagaaggac ggaaaattcg ccggtcgctt
     1981 caggatgcgc ggcctgttgt tcgatgataa agcggcttaa cgtaatgggg aatttcgcca
     2041 tgctgaggag tcctcatgga ttgaacgacg acgagtcgaa atgacgaggg cttcggacag
     2101 agccgaagcc ctccccacga acctggcgcg attatagcgg tttccggcca atcgagcaaa
     2161 ccaggccggc agattatggc tgcgcggcca acccgtagcc ctccgt
//
LOCUS       DAOQEW010000380         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_28599_length_2205_cov_7.532093, whole genome shotgun sequence.
ACCESSION   DAOQEW010000380 DAOQEW010000000
VERSION     DAOQEW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28599_length_2205_cov_7.532093"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLS35_20300"
     CDS             complement(<1..638)
                     /locus_tag="PLS35_20300"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNI21069.1"
                     /translation="MEQDHELARSSKMLSLESRAIDELQRFALTPCYVGVSWGKDSVV
                     IAHLSYRLGIGPLAWFPAGQIENPDCHRVRDAFLKRFPMQYREIEAAPTTDVTEAFHG
                     HDGAKREFEMVSRALGHRYVSGVRADESATRKKRMMRFGESSPNTCAPIGWWPTEYVF
                     AYLAKYDLPTHPVYAMSLGGTYDRNMLRVGTLGGRRGRGNGRKEHEMMYYRD"
     gene            complement(676..1026)
                     /locus_tag="PLS35_20305"
     CDS             complement(676..1026)
                     /locus_tag="PLS35_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21070.1"
                     /translation="MVKVQAVTPLLTKETGGGFRETLAPNDWRGSLHQAVRRWYGSQK
                     WLDKAKFDMLSWSRQRVVVDTRWSGRPVSGWMWEWSGSANAEGLLALRVAEQLGIGST
                     VSRGYGRLRVSAIS"
     gene            complement(1373..2035)
                     /locus_tag="PLS35_20310"
     CDS             complement(1373..2035)
                     /locus_tag="PLS35_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21071.1"
                     /translation="MTPLQIIARLSGQIMSPESCHLDGLLAYALAQVHGLPPVHVSDD
                     RDRDIGFLIAKVSDVLKHDERGFFHCSNPMFEVEQYERRHINRRFPTDRAAVLGADSV
                     RRVELSTGLSKSFRIPTRACFLHRDEVRWFAVGEQSPIERHLRTITHLGKKRAVGLGE
                     VVSWSVTECEGWEHFPILSKDGRPMRSVPIDTPGLTSYRTSLELLCFPYWETSKRRPC
                     AV"
BASE COUNT          482 a          663 c          567 g          493 t
ORIGIN      
        1 tcgtctcggt aatacatcat ttcgtgttct ttgcgcccat taccgcgccc ccttctgcct
       61 cccagagttc ccacccgaag catgttccga tcgtatgtcc caccgagtga catggcatag
      121 actggatgcg tggggagatc gtattttgcc aagtaggcaa atacgtattc cgtcggccac
      181 catccgatcg gcgcgcatgt gtttggagat gattccccaa agcgcatcat acgttttttt
      241 cgcgtcgcgg attcatcagc gcgtacccca gacacgtatc gatggccaag tgcgcggctc
      301 accatttcaa actccctctt agcaccatcg tgaccatgga acgcctctgt tacatcagtc
      361 gttggcgcag cttcaatctc gcggtattgc atcggaaacc gcttgagaaa tgcatcgcgg
      421 acacgatgac aatcggggtt ttcgatttgc cccgctggaa accatgcaag aggaccaatc
      481 cccagtctgt acgataggtg cgcgatcaca acgctgtctt tgccccaact tacgccgaca
      541 tagcatggag tcagtgcaaa tcgttgcaat tcatcgatcg cacgcgattc taaggatagc
      601 attttggagc tgcgtgctag ctcatgatct tgctccaaca agcgctccca tgtctccaaa
      661 tcgcccggtt ggaatctatg agatggcact aacacgaagc ctcccatatc cacggctgac
      721 cgtggaacct atgccaagtt gctcggccac gcgcagcgcc aacaggccct ctgcattggc
      781 tgagcctgac cattcccaca tccagccaga caccggtcgc cccgaccagc gcgtatcaac
      841 cacgacacgc tgacgcgacc aggacaacat gtcaaacttt gccttatcga gccacttttg
      901 cgaaccgtac cagcggcgga ctgcttgatg caacgaaccg cgccaatcgt tcggagcaag
      961 cgtctcccgg aaacctcctc cggtctcctt tgtcagcaga ggagtcaccg cctgcacctt
     1021 taccatagtg gattctgctg tcgggatggg gatccctatt tcgtggaatg gcccgatttc
     1081 gatccgccga atcgcgccga atatctccac tggaaaaacg cgatcactga gcgaatgcgc
     1141 cttttcgtgg gtccagtgag cctcccattc ttctgaccca aacaccgatc cgataggtgc
     1201 cctaggaatt agaatgacat ggggcttttg ccccgtcgtc cgggaggtgt cgtgcccgac
     1261 gttggcgacc gtatgaagca ccccgtgcag tttgatcgaa gtgtcaagac tgactgggtg
     1321 ccgcagagga gcgatgcgca gtgtgctgcg ccatccggga aggcgaagtt gattagactg
     1381 cacagggcct ccttttgctt gtctcccaat acggaaaaca gagcaattcc agcgatgtcc
     1441 gatacgaggt cagaccggga gtatcgatgg gaacggatcg catgggtctc ccatctttgc
     1501 taagaattgg gaagtgctcc cagccctcgc actcggtcac cgaccaggac acaacctcac
     1561 ccaggccaac agcgcgcttt ttcccaaggt gcgtgatggt cctgagatgc cgttcaatcg
     1621 gtgattgttc tccaactgca aaccatcgca cctcatcgcg atgaagaaaa catgctcgcg
     1681 ttgggattcg aaagctcttg cttaacccgg tgctgagttc gacgcgccgg accgagtccg
     1741 ccccaagcac cgcagcacga tcagtcggga aacgtcggtt gatatgccga cgctcgtatt
     1801 gctctacttc gaacatcggg ttggagcagt gaaagaatcc acgctcatca tgtttgagca
     1861 cgtcggacac cttcgcgatc aggaatccta tatctctgtc gcggtcatcg ctcacatgca
     1921 ctggcggcaa tccatggacc tgcgcaagcg cgtaggccaa cagcccatcc agatggcagc
     1981 tttcggggga catgatttgc cctgatagtc tggcgataat ttgcagaggg gtcatgcgtc
     2041 cacctttacc aacaggtctg taagcttttc tgtccgttcc tgcatgtggg ccttagcggt
     2101 ggccagccat ccagcagaca aaccagtgac atcggcttcc gtgcgcatct cggcaaagct
     2161 tgggagtgag cattcctctg cacgaattgc gcgcaactca ccgtg
//
LOCUS       DAOQEW010000381         2178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_29258_length_2178_cov_2.889308, whole genome shotgun sequence.
ACCESSION   DAOQEW010000381 DAOQEW010000000
VERSION     DAOQEW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29258_length_2178_cov_2.889308"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLS35_20315"
     CDS             complement(<1..1087)
                     /locus_tag="PLS35_20315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNI21072.1"
                     /translation="MGPSSRYRVYQFLPHLQQAGIDCHVDPLFGARYFSILRLRSSLL
                     QGCLKVPYVLARFLKRLGTLLTLGRQDLIVIEGQLFPYAPPLIERLLCWLQYRLVFEL
                     DDAIYLTHGHERKMPVLLAMAAGVIAGNDRLAAYATQFSSQVTVVPTVVDTARFVPKP
                     IPTTVARDPHDETITIVWIGLAYNLKYLDVVAPVLRRLQERYPVKLRVVCSQPPALPG
                     VDIEFRPWTWQREVDDLQDATIGVMPLEDTEWARGKCALKLLQYLAVGLPAVASPVGV
                     NSDIIVDGENGFLATTEDEWYERLHALCRQPELRARTGQAGRRTVESHYSLALWGPRL
                     ADVYRSFAECAPNQAAPRPLPRPVSSHG"
     gene            complement(1347..>2178)
                     /locus_tag="PLS35_20320"
     CDS             complement(1347..>2178)
                     /locus_tag="PLS35_20320"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNI21073.1"
                     /translation="IQCPDSVEWYEGDFVNHDDLSAAISGCDILFHLAWTTLPKSSNG
                     NPIYDVETNVLGTLRLLEVASQQKVRKVIFASSGGTVYGIPERIPISESHPTQPLCAY
                     GISKLMAEKYLYLHHAMTGLDYCILRLANPFGERQKPTGSQGVVSVFLDKALRNEKIE
                     IWGDGSVVRDYIYVGDVIDAFIKIATYTGEHRVFNVGSGEGKSLRDVVIAIETMLGRK
                     IACAFSPGRSFDVPSNVLDISKIRTELRWEPRTSFNSGLFRMLSWLSLKCERGNKAIA
                     "
BASE COUNT          537 a          607 c          563 g          471 t
ORIGIN      
        1 gcccatggct cgaaaccggg cgaggaagag gtcgcggcgc cgcttgattc ggcgcacatt
       61 cagcaaagga ccgatacacg tcggcaagcc gaggccccca caaggccaag gagtagtgtg
      121 attccaccgt tcggcggccg gcttgtcccg tgcgcgctcg aagctccggc tgacggcaga
      181 gcgcgtgcaa gcgttcatac cactcgtctt cggtcgttgc gagaaatccg ttctccccgt
      241 ccacgatgat gtcgctattg acgcccacag gcgaggccac cgccggcaat cccactgcga
      301 gatattgcaa cagcttgagc gcacatttgc ctctcgccca ttccgtatcc tccagcggca
      361 tcacaccgat tgtggcgtcc tggagatcgt ccacctcgcg ctgccaggtc catgggcgaa
      421 actcgatgtc cacgcctggg agcgccgggg gctgagagca gaccacgcga agcttcaccg
      481 gataccgttc ttggagccgg cgaagcacgg gcgccaccac atccaaatat ttgagattgt
      541 acgcgaggcc gatccagaca attgtaatgg tctcgtcgtg tggatcgcgt gcgaccgtcg
      601 tcggaatcgg cttcggcaca aatcgcgccg tatccaccac cgtgggaacc accgtgactt
      661 gggacgagaa ttgcgtcgcg taggcggcca accgatcgtt tccagcaatg actcccgccg
      721 ccatggccaa cagcacaggc attttccgtt catggccgtg ggtcagataa atcgcatcgt
      781 ccagttcgaa caccaagcgg tactgcagcc agcacaacag ccgttctatc agcggaggcg
      841 catagggaaa gagttgccct tcgatcacga tcaggtcctg ccggcccagg gtaagaagtg
      901 ttcccagacg tttgagaaag cgggccagca cataaggaac tttcaaacag ccttgcagca
      961 gggaagagcg caatcgaaga atggaaaagt atctggcacc gaacagcgga tccacatggc
     1021 agtcgatccc cgcctgttgg agatgaggca agaattgata gacgcgatag cgactacttg
     1081 ggcccatggg cgagctcttc gaaaaatacg tgaccgtcat ggcgcgtcta tcgccccgtt
     1141 gtctcgaaat atccaccggt tgtgcaggcg aacccgacta ctcaaatagt accggagttc
     1201 tccggcaagg atagattttt cgatgtttcg acggggggag cgagcgagga aatattacgg
     1261 tcactgcgct ctgaggcaat gatacaggga aacgttatgg aacttgctgg tgatctcgag
     1321 acatcagggg cctaggacat caagaattaa gcaattgctt tattccctct ctcgcatttg
     1381 agcgaaagcc agctcagcat tcggaacagc ccgctattga aagatgttcg gggttcccat
     1441 ctaagctcag ttcgaatctt cgaaatatcc aatacattgc tgggaacatc aaagctccgt
     1501 ccaggagaaa atgcacacgc aattttacga ccaagcatag tctctattgc gataaccaca
     1561 tctctaagac ttttcccctc gccacttcct acattgaaca ctcgatgttc cccggtatag
     1621 gttgcgattt ttatgaatgc atcaataaca tccccgacat agatatagtc tcggaccact
     1681 gagccgtcgc cccaaatctc tattttttcg ttcctcagag ctttatccaa aaatacactg
     1741 accacgcctt gagaaccggt cggcttctga cgttccccaa acggattagc caaacgcaga
     1801 atacaatagt ccagcccagt catggcgtga tgaagatata aatatttttc ggccatcagc
     1861 ttgctgatac cataggcaca aaggggttga gtaggatggc tttctgaaat cgggatcctc
     1921 tctggaatac cgtatacagt cccgccggaa gatgcgaata tcaccttcct gaccttttgt
     1981 tgggatgcca cctcaagaag gcgcagcgtc cccagcacgt tagtttccac gtcataaatg
     2041 gggttaccat ttgacgattt tggcaatgtg gtccatgcaa gatggaagag gatgtcacag
     2101 cctgatatcg cggccgataa gtcatcgtgg ttaacaaaat ctccttcgta ccattcaaca
     2161 gagtcaggac attgtata
//
LOCUS       DAOQEW010000382         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_30037_length_2147_cov_9.275813, whole genome shotgun sequence.
ACCESSION   DAOQEW010000382 DAOQEW010000000
VERSION     DAOQEW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30037_length_2147_cov_9.275813"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..90)
                     /locus_tag="PLS35_20325"
     tRNA            complement(1..90)
                     /locus_tag="PLS35_20325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Ser,seq:tga)
     gene            complement(117..1403)
                     /gene="serS"
                     /locus_tag="PLS35_20330"
     CDS             complement(117..1403)
                     /gene="serS"
                     /locus_tag="PLS35_20330"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNI21074.1"
                     /translation="MYDLRVLRDNLDELRDQLGPRGKDVAWEDLRKLTEDRRAQTIQV
                     EDLRHQLKKGSEEVARLKREKQPAEEAMAAMKALGETIKGHEDRLRLIEEQLAHIALR
                     IPNLPHASVPNGHDAADNVEVRRWGTPPNLSAPAQSHWDLGESLGILDFDRAVRMAKA
                     RFAVLTGRGARLERALINYMLDMHTTQHGYREVMPPLMVNRAAMTGTGQLPKFEEDLF
                     QMRDEELFLIPTAEVPVTNLHREEILAEEALPIRYTAYTPCFRREAGSYGKDTRGLIR
                     LHQFNKVELVAFAKAEHSYDELERLTSHAEAVLQGLGLHYRVITLCKGDMGFSSAKTY
                     DIEVWLPSQQTFREISSCSNFEAFQARRANIRCRPKAGKKDSKPEFVHTLNGSGLAVG
                     RTLVAILENYQQQDGSVAIPAVLQPYMGGLQTISRS"
     gene            1530..>2147
                     /locus_tag="PLS35_20335"
     CDS             1530..>2147
                     /locus_tag="PLS35_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNI21075.1"
                     /translation="MIAVKRISNPPNRFEPLEREALEPSLRARIQLFEDNTHQILSQN
                     DSPDLPFKWSVNPYRGCFHACAYCYARPSHEYWGFGAGTDFESKIVLKRRAATLLRQS
                     FEKPSWKGELVVFSGNTDCYQPLEATLGLTRACLQVCAEYRNPVGIITKGVIPTRDLD
                     LFTQLRERAWIRVYFSIAFADDETARLVEPQAPGISARFEAMRVLS"
BASE COUNT          437 a          617 c          587 g          506 t
ORIGIN      
        1 tggcggaggg ggcgagattt gaactcgcgg ataggttacc ccatctccgg ttttcaagac
       61 cggcacgttc ggccactccg tcacccctcc acacctggcc cggacactta tgaccatcag
      121 gatcgcgaga tcgtctgaag ccctcccata tagggttgta acactgctgg aatagccaca
      181 ctgccatcct gctgctggta attctcgaga atagccacca gcgttctgcc cacggccaga
      241 cccgatccgt ttagcgtatg aacgaattcc ggtttggagt ctttttttcc tgctttggga
      301 cggcaacgta tgtttgctcg ccgtgcttga aacgcttcga aattgctgca ggacgagatt
      361 tcacgaaacg tctgctgcga cggcaaccat acctcaatgt cataggtctt cgcggaagaa
      421 aatcccatgt cgcctttgca gagcgtgatt actcgataat gcaatcccag cccttggagc
      481 accgcttccg catgggacgt gaggcgctcc agttcatcgt aagagtgttc ggccttcgcg
      541 aaggcgacga gttccacttt gttgaattgg tgaaggcgga tgaggccgcg cgtgtctttg
      601 ccgtaggagc ctgcttcgcg tctgaaacaa ggcgtgtagg ccgtgtaccg aatcggcaag
      661 gcctcttccg ctaatatctc ttctcggtgc agattagtga cgggaacttc cgccgtagga
      721 atcaggaaca gttcctcgtc gcgcatctgg aacaaatctt cttcgaattt gggtaattga
      781 cccgtcccgg tcatcgccgc gcgattgacc atcagcgggg gcataacttc gcgatagcca
      841 tgctgcgtgg tatgcatgtc cagcatatag ttgatgagcg cacgctcgag tcgagctccg
      901 cgtccggtca ggacggcgaa gcgagccttg gccatgcgca cagcccggtc gaaatcgaga
      961 atgcctaacg actccccgag atcccaatgc gactgcgccg gcgctgagag gttcggcggc
     1021 gttccccatc gacggacttc cacattgtct gcggcgtcat gaccattcgg cacagaggca
     1081 tggggaagat tgggaatacg cagcgcgata tgcgctagct gttcttcgat caatcgaagc
     1141 cggtcttcat ggcctttgat ggtttctcct aaggctttca tcgccgccat ggcctcttca
     1201 gccggctgct tctcccgctt aagtcgggcg acttcctcgg agcccttttt gagctggtgc
     1261 cgaaggtctt caacttgaat cgtctgggca cgacgatctt cggtcagttt tctcaaatct
     1321 tcccaggcaa cgtccttccc ccgtggccct agctggtcac ggagttcgtc cagattgtct
     1381 cgcaataccc gaaggtcgta catactcttt cccctagaag cggcgtgaaa tttaacaccc
     1441 gatgcctgcg tagtcaatga aactggtcgc tgatcgctgg aaccgaaggc gcttgggcgt
     1501 cattgacaac gcgcgggtgg ggcggcacca tgattgctgt gaaacggatt tcgaatcctc
     1561 ctaatcgatt tgaacccctt gagcgcgaag cgcttgaacc atcgctgcgc gcccgtattc
     1621 agctgttcga agacaacacc caccagattc ttagccagaa tgacagcccg gacctcccgt
     1681 tcaaatggag cgtgaatccc taccgggggt gttttcatgc ctgcgcctac tgttacgcgc
     1741 gcccatctca tgagtattgg ggattcggag ccggaactga tttcgagtcg aaaatcgtgc
     1801 tcaaacgacg ggcggcaacc ttgctgcgac agtcgttcga gaagccctcc tggaagggcg
     1861 agttggtggt gttttctggc aataccgatt gttatcaacc cctcgaggcc accctcggtt
     1921 tgacgagagc ctgtcttcaa gtctgcgccg aataccggaa tcctgtaggc atcatcacaa
     1981 aaggagtcat tccgacacgt gatctggatc tgttcacaca attgcgcgaa cgggcatgga
     2041 ttcgcgtcta cttcagtatc gcctttgccg atgatgaaac cgcgcgcctg gtggaaccgc
     2101 aggctcccgg cattagcgca cgctttgagg cgatgcgtgt gctctca
//
LOCUS       DAOQEW010000383         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_30324_length_2135_cov_7.481731, whole genome shotgun sequence.
ACCESSION   DAOQEW010000383 DAOQEW010000000
VERSION     DAOQEW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30324_length_2135_cov_7.481731"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2135
                     /locus_tag="PLS35_20340"
     CDS             <1..>2135
                     /locus_tag="PLS35_20340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNI21076.1"
                     /translation="GSIALSMALVLVADVYTPLGVTVWVLYLVPLVLSYLTWSPMVPV
                     GVGATVSLFTLVGLVISPSGIGPTLSLINRTMGIVTAGALATFGYQFITSKLALRKEE
                     WIRVGQTRLSERMLGEQSLITLGQNVLHELAEYMDAQGGAIFVADGSRFHRVATHGVP
                     EGTSLPDTFQAGDGLLGQALHAKRPLVVQDLPEGYFTVGSALGRSAPRHLLIAPFGVD
                     GLTNTVVELAFFHPVHQSDRELIDRVAESIAVAMRSAHYRSRLQELLEETQRQAEELQ
                     VQSEELRVANEELEEQSRALKESHGRLEQQQAELEQTNSQLEEQAQILETQKDELSEA
                     ALISNAQKQQLERVSQYKSEFLANMSHEMRTPLNSTLILAKLLADNPQGNLTADQVKS
                     ASTIESAGHDLLTLIDDVLDLAKVEAGRIDITPGDVSLQRLLDTLHALFQPLAAQKQI
                     QLRTTRAPGTPSSIQSDWQRLEQVLKNLLSNALKFTEAGQVSLDISMLPDGRLAFAVQ
                     DTGVGIPAAQQDLIFEPFCQGDGTTSRKYGGTGLGLSISREFVRLLGGELHLASTPGE
                     GSTFTLILPQTLTMVPPPAPGVTTLSPTVTEPLPPASPHHHHTAAAPLRTDDDREGLV
                     PHRRVILIVEDDESFARVLADLAHELNFQVLLSNTAADAFSLASRYLPSAVILDIGLP
                     DHSGLSVIDRLKSDVRTRHIPVHVVSGHD"
BASE COUNT          445 a          665 c          598 g          427 t
ORIGIN      
        1 ggatccattg ccctctcaat ggcattggtg ctcgtggcgg atgtttatac cccgctgggg
       61 gttaccgtct gggtgttgta tctcgttcca ctggtgctgt cctacctgac ctggtctccc
      121 atggtgcccg tgggtgtcgg cgcaaccgtg tccctcttca cgctcgtggg tctcgtcatc
      181 agcccatccg gcatcggtcc taccctgtcg cttatcaacc gaaccatggg aatagtcacg
      241 gccggcgcat tggcgacatt tggataccag ttcatcacca gcaagctggc gctgcggaaa
      301 gaagaatgga tacgcgtggg ccagaccagg ctgagcgaac gcatgctcgg ggaacagagc
      361 cttatcaccc tcggtcagaa tgtgctgcat gagctcgccg agtatatgga tgcccagggc
      421 ggcgcaattt tcgtcgccga cggctccaga ttccatcgtg tcgcgaccca tggagtgccg
      481 gaaggtacat cgctcccgga cacgttccag gctggtgatg gattactcgg tcaagcactt
      541 cacgccaaac gcccgttggt cgtgcaagat ctgccggagg ggtactttac cgtcggatcg
      601 gcactcgggc gaagcgcccc tcggcatctg ttaatcgcgc cattcggtgt tgatggtctg
      661 accaataccg tcgtggaatt ggcgttcttt catccggtgc atcaatcgga cagagagttg
      721 atcgatcggg tcgccgaatc catcgccgtg gccatgcgat ccgctcacta ccgaagccgg
      781 ttgcaggaat tgctcgagga aacgcagcga caagccgaag agcttcaggt gcaaagcgaa
      841 gaactgcggg tcgccaatga ggaattggag gagcagagcc gcgcgctgaa ggaatcacac
      901 gggaggctcg aacagcaaca ggcggagttg gaacagacga attcccaatt ggaagaacag
      961 gcgcagattc tcgaaacaca aaaagatgaa ctcagcgaag cggctctcat ctcaaacgcc
     1021 caaaaacagc aattggaacg ggtgagccag tacaagtcgg aattcctggc caacatgtcc
     1081 catgaaatgc gcacgccgct caactccacc ttgatcctcg ctaagctgct ggccgataac
     1141 ccacagggca atctgacggc cgatcaggtc aaatcagcct cgaccatcga atcggccggc
     1201 catgacctgt tgaccttgat cgacgacgtg ttggacctgg ccaaggtcga ggccggccgc
     1261 atcgacatca caccgggcga cgtctcgctg caacggttgc tcgataccct ccacgcgctc
     1321 ttccagccgc tggcggcgca aaaacagata cagcttcgca ccactcgcgc acccggcacg
     1381 ccgtcatcca ttcaatccga ttggcagcga ctcgaacagg tgctgaagaa tctcttatcc
     1441 aacgcgctca agtttacgga agccggccag gtgtcgctcg acatttcgat gctccccgat
     1501 ggccggctgg cctttgccgt gcaggataca ggggtcggga ttccagcggc ccagcaggac
     1561 ctgatcttcg aacccttttg ccagggtgac ggtacgacga gccggaaata cggaggcacc
     1621 ggtctgggat tatcaatctc ccgcgaattt gtccggttgc tcggcggaga actccacctc
     1681 gccagcactc caggggaagg cagtaccttc acgcttatcc tgcctcaaac cctgacgatg
     1741 gttcctccgc ccgcccccgg cgtgacaacc ctgtcgccga ccgtgacgga gccccttcct
     1801 ccggcgtcgc ctcaccatca tcacaccgcc gcggcaccct tacgaaccga tgacgatcga
     1861 gagggcctgg tccctcaccg ccgggttatt ctgatcgtgg aagacgatga gtccttcgct
     1921 cgcgtcctcg ccgacctggc tcacgaattg aattttcaag tgctgctcag caacacggca
     1981 gccgatgcct tctcactggc ctcacggtat ctgccgagcg cagtcattct cgacatcgga
     2041 ttgccggatc attccggact ctcggtcatc gatcgtttga agagcgatgt ccggacccgg
     2101 catatccccg tccatgtggt gtccgggcat gacta
//
LOCUS       DAOQEW010000384         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_30482_length_2129_cov_6.462392, whole genome shotgun sequence.
ACCESSION   DAOQEW010000384 DAOQEW010000000
VERSION     DAOQEW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30482_length_2129_cov_6.462392"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1036)
                     /gene="hemH"
                     /locus_tag="PLS35_20345"
     CDS             complement(92..1036)
                     /gene="hemH"
                     /locus_tag="PLS35_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNI21077.1"
                     /translation="MSAAPRPIALLLMAMGGPDSLENVEPYLLDVRGGRPTPPELVEE
                     IRTRYRVTGGKSPVLDITREAAAALERRLNASGERRFRCYVGLRHWHPFIRDTYAELL
                     KADPDHIVGLCMAPQYSSLSIGAYVKKVEEARADLADETPISYVTSWHRHPLLIAAIV
                     AHIRHTLERFPVEVRAQVSVLFTAHSLPERVVAMKDSYPEEVGATVQAVCAQLGGQPT
                     RFAYQSQGRSGEKWLGPSVEETLEDLAQAGHHHVLVAPIGFLCDHVETLYDIDIELTQ
                     LARTKGIQLERIPMLNASAPLIDILTSVVETHESSLVH"
     gene            complement(1122..>2129)
                     /gene="hemE"
                     /locus_tag="PLS35_20350"
     CDS             complement(1122..>2129)
                     /gene="hemE"
                     /locus_tag="PLS35_20350"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNI21078.1"
                     /translation="KACRREPVDCTPVWFMRQAGRYMIEYRRLREKHSILDLCKTPEL
                     AAQVTLQPIDRFALDAAIIFADILLPLEPMGLSLEFAEGEGPIIHNPLRDRAAVERLK
                     MLDDSDLQYVMDAISLTRKMLAGRVPLIGFAGAPFTLASYAIEGGSSRNYIHTKQMMY
                     GEPEVWHRLMDKFARAITGYLRRQIKAGAQAVQLFDSWVGCLSAGDYAEYVMPHVQLI
                     FDGLKHEGVPLIHFGTGTTAILKSMRQAGGDVIGVDWRIPIDEAWAMVGYDRAVQGNL
                     DPVTLFGPMHEIERRVTDILRRAGGRPGHIFNLGHGILPNTPVEHVAATIDLVHKLSA
                     R"
BASE COUNT          448 a          683 c          603 g          395 t
ORIGIN      
        1 tgctccagga gagcaaaggc cgtggagagg cctgagatgc ctccaccgat aatgaccact
       61 gaccgtgaag tgcgcgccac aggtggtgcc gctaatgaac gagcgaggac tcgtgcgtct
      121 ccacgaccga cgtcagaatg tcgatcaggg gggccgaggc gttcaacatg ggtattcgtt
      181 ccagttggat ccccttcgta cgggccaact gcgtgagctc gatatcgatg tcgtacagcg
      241 tttccacatg gtcgcaaaga aaaccgatgg gcgccacaag cacatggtga tgaccggctt
      301 gcgccaagtc ttccagcgtc tcctcgacag aaggcccgag ccacttctcg ccggagcgcc
      361 cctgactttg ataggcgaac cgcgtcggtt gtccgccgag ttgcgcacat acggcttgga
      421 cggtagcgcc gacttcctcc gggtaggaat ccttcatggc caccacccgc tccggcagac
      481 tatgggccgt aaagagcacg gacacttgcg cgcggacctc cacgggaaat cgctccagcg
      541 tatggcggat atgcgcgacg atcgcggcga ttaacaacgg atgacggtgc cagctcgtca
      601 cgtagctgat cggcgtctca tctgccaggt ccgcacgcgc ctcttcgacc ttcttcacat
      661 aggcgccaat gctgagcgac gagtactgag gcgccatgca caagcccacg atatgatcag
      721 gatccgcctt gagcagttca gcgtaggtgt ccctgatgaa cggatgccag tgccgcaatc
      781 ccacgtagca ccgaaaacgc ctttcgccgg atgcgttcag tcgccgttcc aaggcggcgg
      841 ccgcctcgcg cgttatgtcg agtacaggag acttcccacc cgtcacacgg taccgcgtcc
      901 ggatttcctc caccaactcc ggaggggtcg gacgcccgcc gcggacatcc agaaggtacg
      961 gctctacatt ctccagacta tcgggcccgc ccatggccat cagcaacagg gcaatcgggc
     1021 gtggtgctgc cgacatccga gacctcgtca gccgtgacca ggaagacgtc tctcgcgaat
     1081 caagcgcctg ccgggcaatg agcgcctacg tctcaccaga ttcagcgggc gctcaactta
     1141 tggaccagat cgatcgtggc ggccacatgc tccacgggag tattggggag gatcccatgc
     1201 ccgagattga aaatgtgccc gggtcgccct ccggcccgcc gaagaatatc cgtcacgcga
     1261 cgctcgattt cgtgcatcgg cccaaacaag gtgaccggat cgaggtttcc ctgcacggcg
     1321 cgatcgtatc ccaccatggc ccaggcctcg tcgatcggga tacgccaatc gacgccgatc
     1381 acgtcaccac cggcctggcg catcgatttg agaatggccg tcgtacccgt cccgaaatga
     1441 atcagcggca caccttcgtg cttgaggccg tcaaaaatca attgcacatg cggcatcaca
     1501 tattcggcat agtctcccgc tgagaggcag ccgacccaac tgtcgaagag ctgcacggcc
     1561 tgggcgccgg ccttgatctg ccgacgcaaa tacccggtga tcgctcgcgc gaacttatcc
     1621 atcagacggt gccagacttc cggctcgcca tacatcatct gcttggtgtg gatatagttc
     1681 cgtgaactgc cgccttcgat cgcataactg gccaaagtaa acggcgctcc ggcaaagccg
     1741 atcagcggca ctcgaccggc caacatcttg cgcgtcaggc tgatggcatc catgacatat
     1801 tggaggtcgc tgtcatcgag catcttgagc cgctccaccg ccgcccgatc ccgaagggga
     1861 ttatggataa tcggcccttc cccctccgcg aactccagcg acagtcccat cggttccagc
     1921 ggcagcagaa tgtcggcaaa aataatggct gcatcgagcg caaaccggtc aatgggctgc
     1981 agggtgacct gagctgccaa ttccggcgtt ttgcacaggt caagaatcga gtgtttttcc
     2041 cgcaatcgac ggtattcgat catataacgg cccgcctggc gcatgaacca gaccggcgta
     2101 caatcaaccg gttcgcgccg gcacgcttt
//
LOCUS       DAOQEW010000385         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_31288_length_2100_cov_9.240587, whole genome shotgun sequence.
ACCESSION   DAOQEW010000385 DAOQEW010000000
VERSION     DAOQEW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31288_length_2100_cov_9.240587"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..321
                     /locus_tag="PLS35_20355"
     CDS             76..321
                     /locus_tag="PLS35_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNI21079.1"
                     /translation="MIQADVKLDTLGYFCPMPIILTSKKIKELTMGQVLEVVSDDEGI
                     KKDMPAWCETTGHQMVGLEEEQSSSKRIYKAFVKKAK"
     gene            459..2021
                     /gene="ftcD"
                     /locus_tag="PLS35_20360"
     CDS             459..2021
                     /gene="ftcD"
                     /locus_tag="PLS35_20360"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNI21080.1"
                     /translation="MSQVIECVPNFSEGRNPDVIQALVALVRSVPGVALLDESHDTDH
                     HRSVVTFAGRPYAVAEVAFQMARLASQLIDLRSHHGEHPRVGATDVMPFVPIREVSMQ
                     DCVQLARMVGQRIGNELKIPVFLYEQAAARPERVQLEWIRNGGLKGLADRMAADPAWV
                     PDFGPKQLHPTAGATIVGARWPLIAFNVNLKSGDLLVAKAIAKAVRQSSGGLPSVKAI
                     GVELRSQGLVQVSMNLTNYEETSLQVAFTAVEREAAARGVEVAGTEIIGLVPEQCLID
                     TAQRALRLDRFEGRQVLEMRLDSAESRAAIGRLAGSQPVKESPSLPAKPVASTNVLAA
                     EQGLTGGSVGALAASFAASLGTMVAKLNRARPAETRLTEIGTRFRELAQADRDAYARV
                     LKARKLPPNHPDRAVQIASGLLDAIETPMEIVKLCCEVIPLLRGLLAQAKPELQPDLK
                     MGIELADAAINGCVAMVEENMKVQPNQLLVASIRERFAVAEQMLVDAKSLCYTPPFDS
                     WSQNMLNILKLR"
BASE COUNT          456 a          548 c          667 g          429 t
ORIGIN      
        1 ggtgtcacca cctacctggc ggaagccaag cagggcagtg tcaatctgtt tatctaagcg
       61 ggctgaggag agggcatgat acaggctgat gtgaaactcg atacgctggg gtacttctgc
      121 ccgatgccga ttattttgac ctcgaagaag atcaaggaac tgaccatggg tcaggtgctg
      181 gaagtcgtct cggacgacga aggcatcaag aaagatatgc cggcctggtg tgagacgacc
      241 ggccatcaaa tggtgggatt ggaagaggag caaagttctt ccaaacggat ttacaaagcc
      301 tttgtcaaaa aggccaagta gcactcagcg acaggcgcgc ggatgcgaag ccgtcatgtt
      361 ttgccaagcc ggagggtatg gtcgcctgcc tccggctttt ttttgtctga gactgtcctg
      421 gccgtaagtc ggccctgcac accagaagaa ggaatgccgt gagtcaggtg atcgaatgtg
      481 tgccgaattt tagtgagggc cggaacccgg acgtcatcca ggccctcgtg gccctggttc
      541 gatccgtccc aggcgtagcc ttgctggatg agtcgcacga tacggatcat catcgttcgg
      601 tggtcacctt tgccggacgg ccctatgccg tcgccgaagt ggcctttcaa atggcgcgac
      661 tcgcttctca gctgatcgat ctgcggagcc atcatggtga acatccgcgc gtgggcgcca
      721 cggatgtgat gccgttcgtt ccgattcgcg aggtcagcat gcaggattgt gtacaattgg
      781 ctcgcatggt cggccagcgc attggcaatg agctgaaaat ccccgtgttt ctctacgagc
      841 aggcggcggc gaggccggag cgcgtccagc tggaatggat ccgaaacggt ggcctgaagg
      901 gactggccga ccggatggcc gcggacccgg cctgggtgcc cgacttcggt ccgaagcagc
      961 tgcatcccac cgccggcgct acgatagtcg gcgcgcggtg gccgctcatc gcctttaacg
     1021 tcaatctgaa gagtggggac ctgttagttg ccaaggcgat cgcgaaggct gttcggcagt
     1081 cgagtggagg tcttccttcg gtgaaagcga tcggggttga actgaggagc caggggctcg
     1141 tccaggtctc gatgaatctg acgaactatg aggaaacctc gctgcaggtg gcctttaccg
     1201 cggtggagcg ggaagcggca gctcgcggag tggaagtggc ggggacggag attatcggtc
     1261 tggttcctga gcaatgcctc atcgataccg cgcagcgggc gcttcgtctc gatcgatttg
     1321 aaggcaggca ggtcctcgaa atgcgattgg acagtgccga atcgcgcgca gccatcggga
     1381 ggctggccgg gtcgcaaccg gtgaaggagt caccgtcgtt gcccgcgaag cccgtcgcat
     1441 cgacgaatgt tcttgccgct gagcaaggat tgacgggcgg cagtgtaggc gccctcgcgg
     1501 catcgttcgc ggcgtcgtta ggcaccatgg tggctaaact gaaccgcgca cgtccggccg
     1561 aaacacgcct cacggagatt ggtacgcgct tccgcgagtt ggcgcaagcc gaccgggatg
     1621 cctacgctcg agttctcaag gcaaggaagc ttccgcctaa ccatcctgac cgagcggtgc
     1681 aaatagcctc cggtttattg gacgccatcg aaacgccgat ggaaattgtg aaactctgtt
     1741 gcgaggtgat cccgctgctg cgcggtctgt tggcgcaggc aaaaccggag cttcagcctg
     1801 acttgaagat gggaattgaa ctggcagatg ccgcaatcaa tggctgtgtg gcgatggtcg
     1861 aagaaaacat gaaagttcaa ccaaatcagc tacttgttgc atccattcgg gaacggtttg
     1921 ccgtagcgga acaaatgctt gtggatgcga aatcgctatg ctacactccg cccttcgatt
     1981 cgtggtccca aaacatgttg aatatcctga aacttcgctg aaagagagtc gaaggtagct
     2041 gacgatggaa attaaggtct tcaataacaa cgttgaaaaa gccctgaagg tcgccaagaa
//
LOCUS       DAOQEW010000386         2098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_31352_length_2098_cov_6.337739, whole genome shotgun sequence.
ACCESSION   DAOQEW010000386 DAOQEW010000000
VERSION     DAOQEW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31352_length_2098_cov_6.337739"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="PLS35_20365"
     CDS             complement(<1..2041)
                     /locus_tag="PLS35_20365"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HNI21081.1"
                     /translation="MESAPRVCPDFYSSLILPAYSTGQPDYEGAMRHLRTQLQLDPAT
                     GDEEGLTEDIRANLLLSVGQSVRDMINNVLLIHAWKNAESADAKRRAATADTVLLQQQ
                     AIQKDLKFQSYSKSYVAQKVVPLLRTIVEGVVYIMTPFLLVLAISPGMSRLMGTYWRL
                     FMWLQTWSPLYAVLNFVMFWEARSRLGAILSSNGVGQSVNYATLDRINEHVAMLNSTA
                     ADYVWLVPAIGFGLMWGGSQVLGSILAGSRTAETVAGQVGGQFSRGLGANLEEGPSYR
                     HEQRGAQFGEMIGHDVEPQMTVPTGQGGFRVDSQSGTSEIHDSQGGITSIGANGIQNY
                     KGPEGQYAMDSKGHLLSGVFRHKMFDPQSGKQVDAQTSVFGSMVEHRFDTMGEDGSRK
                     SVLVQENQDTGELVARTEQSVRKGLSETRTIAEDGSQSFARSGNVPLFLEVAGETRPD
                     AGFVQMMGERGPGERVDTLTQSTFQSTMGAKQSYTGRLVERDGQFHFLATEGGAVRGF
                     DNKDTEAGVHRESFVSPDGSNYFVETGQAEQMVLGKDGVTRPMEGYVRAFGSVDRSDP
                     VHPRDIYSSYSLTSDEGGHTHTVTGTLGTDASGRQFLVEATEHFESGTSGVAHDTHTV
                     GSYTLSGTFTALSHGEANQPGVPTAFFGSAIDNSKPGSPAFQANALLYDGQVSFFD"
BASE COUNT          448 a          664 c          522 g          464 t
ORIGIN      
        1 cgtcaaaaaa gctcacctgg ccgtcgtata acagcgcatt ggcttgaaac gccggagaac
       61 ctggcttgct attgtcgatg gcagagccga agaaggctgt cggcactcct ggttgattgg
      121 cttccccatg actcagagcc gtgaacgtgc cgctcagggt gtaggaaccg accgtgtggg
      181 tgtcgtgggc aacaccgctc gtgcctgact caaaatgttc cgtggcttcc accaagaact
      241 gtcgtcctga tgcatcggtg cccaatgttc ctgtcactgt gtgcgtgtga ccaccctcat
      301 cactggtcag actgtaactg gaatagatgt ctcgcggatg caccgggtcc gagcgatcga
      361 ctgacccaaa cgccctgaca tatccctcca tcggccgagt caccccatct ttccctaaga
      421 ccatctgttc cgcttgccca gtctccacaa aataattgga accgtcaggg gaaacgaacg
      481 attccctgtg gacgccggcc tccgtatcct tgttgtcaaa tcctcggacg gccccgccct
      541 cggtcgccag aaaatgaaac tgtccatcgc gttctaccag tcgcccggta tacgattgtt
      601 tggcacccat cgtgctctga aatgtcgact gtgtgagggt gtctacccgc tcgcctggcc
      661 cacgctcacc catcatctgg acaaatccgg catcgggacg tgtctcgccc gcgacttcca
      721 aaaacaacgg cacattcccc gatcgtgcaa aactttgact accgtcttca gcaatcgtac
      781 gcgtctcgga caggcctttc cgaacggatt gttccgtgcg cgccactaat tctccggtat
      841 cctgattttc ctgcacaagc acgctcttcc tggagccatc ctcccccatc gtatcgaagc
      901 gatgctccac catcgaacca aacaccgagg tttgtgcatc gacctgcttc ccggactgtg
      961 gatcaaacat cttgtgacgg aacaccccac tcagcagatg cccctttgaa tccattgcat
     1021 actgtccttc tggccccttg tagttctgaa tgccgttcgc gccaatactg gtgatgccgc
     1081 cctgggagtc gtggatctca ctcgtgccgg attgactatc cacacgaaac ccaccctgac
     1141 cagtcggcac agtcatctgt ggttcgacgt catggccgat catctcgccg aactgggccc
     1201 cacgctgttc gtgtcgatag gacgggcctt cctccaaatt ggcaccaagc ccacgtgaga
     1261 actgtcctcc cacttgcccc gcgacggttt ccgcagtccg cgatcctgcc aggatcgatc
     1321 ccaacacctg gcttccgccc cacatcagac cgaaaccgat cgctggcacg agccacacat
     1381 aatctgcggc tgtggaattc agcatggcga catgttcatt gattctgtct aacgttgcat
     1441 agttgacgga ttggccaacc ccgtttgagc tcaggatcgc gccgagccgg cttcgcgctt
     1501 cccaaaacat gacaaaattc agcacggcat acagaggcga ccaggtctgg agccacatga
     1561 acaagcgcca ataggtgccc atcaaccgtg acatcccagg cgaaatggca agcactagga
     1621 ggaacggggt catgatgtac accacccctt ccacaatggt ccgtaatagc ggtacgacct
     1681 tctgagcgac ataggacttc gagtagcttt ggaactttaa gtccttttgg atcgcctgct
     1741 gctgcagaag cacagtgtcc gcagtcgccg cgcgccgttt ggcatcggcg gattcggcgt
     1801 ttttccacgc atggatgagc agcacattat taatcatgtc tcggaccgat tgcccgacgg
     1861 ataacaagag attcgcccgg atgtcctcag tgagcccttc ctcatcgccg gtggccggat
     1921 caagctgcaa ctgtgtccgc aaatgccgca tcgccccttc gtagtcaggc tgtccggtgc
     1981 tatacgcagg aaggatgaga gaactataga agtcgggaca cacccgcggg gccgattcca
     2041 acgtcccacc tctatatccg agtgccgtga gtgcgttatt cgtagccccg atatcatt
//
LOCUS       DAOQEW010000387         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_31630_length_2089_cov_6.554081, whole genome shotgun sequence.
ACCESSION   DAOQEW010000387 DAOQEW010000000
VERSION     DAOQEW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31630_length_2089_cov_6.554081"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /gene="istB"
                     /locus_tag="PLS35_20370"
     CDS             <1..551
                     /gene="istB"
                     /locus_tag="PLS35_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNI21082.1"
                     /translation="LRFVESAHNVVLVGGPGTGKTHLATSLGIEAIRMHGKRVRFFST
                     VELVNALELEKAQNKAGQMAHRLMYVDLVILDELGYLPFTQSGGAMLFHLLSKLYERT
                     SVVITTNLTFSEWSSVFGDAKMTTALLDRLTHHCHIVETGNESWRFKHSSAAGAAPPK
                     QRPKSQKGDRKTADPIDLSTTE"
     gene            626..946
                     /locus_tag="PLS35_20375"
     CDS             626..946
                     /locus_tag="PLS35_20375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNI21083.1"
                     /translation="MVLCDPQVYEGYYRELARLLRPLERDPDIAEHVSSLDMMLTLVG
                     AGYGVGFIRATRIAASLRPDVVIRPLAMDSAFITTYLLRPVSVDMPVSAERFIARLRA
                     HSGD"
     gene            complement(984..1304)
                     /locus_tag="PLS35_20380"
     CDS             complement(984..1304)
                     /locus_tag="PLS35_20380"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI21084.1"
                     /translation="MTLLSMQKLTIQPGRPPGTPTYESEPALAFGRAVRAARVAQSVA
                     QDDFAFQAGISRSHMGKIERGEHVPTLPLILKIAAALGISASELMAATEKNLNSGSEP
                     QGSP"
     gene            1379..2056
                     /locus_tag="PLS35_20385"
     CDS             1379..2056
                     /locus_tag="PLS35_20385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNI21085.1"
                     /translation="MTYFVASVGSPWTTSPASQPAHLRLAIATGVSSSQLSALLALQR
                     AQEPEVRIAFFEVAGDELLDGLREGRYDVGVSLQGSSDPAIQTQPLWNEHMAVAMPLR
                     FPLLDQASLTIADLQDYPIFRWRAETCSSLDHRLLSRPPADPQKPQYVTSFEMMALWV
                     SAGYGVGLSAQSRIKHAHGWGVSMRPLDDGPYEIVTHLHRPQGQANSVSERLERRALQ
                     IAKSVAS"
BASE COUNT          419 a          630 c          611 g          429 t
ORIGIN      
        1 cccttcgctt cgtggagtcg gcgcacaacg tggtgctggt tggtggcccc ggcacgggta
       61 aaacccacct ggccacgagc ctggggatcg aggccattcg catgcatggc aagcgggtgc
      121 gcttcttctc aacggtggag ctggtcaacg ccctggagct ggagaaggcg cagaacaagg
      181 ccgggcaaat ggcgcaccgc ctgatgtacg tcgatctggt gatcctggat gaactgggct
      241 acctgccatt cacgcagtcg ggcggcgcga tgctgttcca cctgctctcc aaactctatg
      301 agcgaacgag cgtggtgatc accaccaacc tcaccttctc ggagtggagc agcgtctttg
      361 gagacgccaa gatgaccacg gccttgcttg accggctcac gcaccattgc cacatcgtcg
      421 agacgggcaa cgagagctgg cgattcaagc actccagtgc cgctggcgcg gctcccccaa
      481 agcaacgccc aaagagccag aaaggagatc gaaaaacagc agacccgata gacttatcca
      541 caaccgaata gtcaaagaat ccatcaaccc agtggctcaa tattcaacgt gatcactggc
      601 tcagttgtgc tgatgaatca acacactggt cttatgcgac ccacaggtat acgaaggcta
      661 ctaccgtgaa ctggcgcggc tcctgcgacc gctggagcgt gatcccgaca tcgccgagca
      721 cgtgtcctcg ctggacatga tgctcaccct ggtcggtgca ggctacggcg ttggcttcat
      781 cagggcaacc aggatcgcgg cgagcctgcg ccccgatgtg gtgatccgcc ccctggccat
      841 ggattccgca ttcatcacga cctacttgct tcggcccgtc agcgtggata tgccggtgtc
      901 ggcggagcgg ttcattgcgc gcctgcgcgc gcactcgggc gattgatcgg gcggcaaggg
      961 ctgctactga agttcgcggc tgtttatggc gagccttgcg gttcactgcc agagttgagg
     1021 ttcttttcag tcgccgccat caactcactg gcgctgatcc cgagcgcggc agcaattttc
     1081 agtatcaggg gaagcgtcgg cacatgctcc ccgcgctcga tcttgcccat gtgtgaacgc
     1141 gatattccag cctgaaatgc aaagtcgtct tgagcgacgc tttgcgcgac gcgagcggca
     1201 cgcacggccc gcccgaaagc taatgctggt tcggattcat aggtgggtgt gccaggtgga
     1261 cgacctggct gaatagtaag cttctgcatc gacagaagcg tcaaacaatc atcttaaatt
     1321 aaccacgtta aacatataga cgttaaagtt ctctctttac tatgatgctg gtttgccctt
     1381 gacttacttc gtcgcttctg tgggttctcc atggacaaca tcaccagcca gccagccagc
     1441 ccacctcagg cttgcgatag ccaccggcgt atcgtcatct cagctttcgg cgctgctcgc
     1501 gctgcaacgt gcgcaagagc cagaggtccg catcgcgttc ttcgaggttg caggtgacga
     1561 gttgcttgat ggactccgtg aaggccgcta cgacgtaggg gtgtcgcttc aaggatcgag
     1621 cgatccggcc atccaaaccc aacccttgtg gaatgagcac atggccgttg cgatgccgct
     1681 gcggtttcca ttgctggatc aggcatcgct caccatcgcc gatctacagg actacccgat
     1741 ttttcgctgg agggctgaga cgtgttcttc gctggatcat cggctgctct cgcgcccgcc
     1801 tgccgacccg caaaaacctc agtacgtgac gtcgttcgag atgatggcgc tatgggtttc
     1861 tgccggctac ggtgtcgggc tatccgcgca atcgcgcatc aagcacgccc atggatgggg
     1921 agttagcatg cgtcccctgg acgacggccc ctacgagatc gtgacgcacc tgcataggcc
     1981 ccaggggcaa gcgaactcgg tttccgagcg actcgagcga agggcgctgc agatcgcaaa
     2041 atcggtcgct tcctgatcgc cgtggctgtc ggtagccgcc agcggaagg
//
LOCUS       DAOQEW010000388         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_32275_length_2067_cov_10.390159, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000388 DAOQEW010000000
VERSION     DAOQEW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32275_length_2067_cov_10.390159"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..684
                     /locus_tag="PLS35_20390"
     CDS             49..684
                     /locus_tag="PLS35_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HNI21086.1"
                     /translation="MTQYRVLPGPEHFLPPAAASMGIYLPNPGEAHINGVIVPEEKAY
                     EEAARQFLMAQVPTIFPGPLVLWAWNEKAAKKAAAVRSLYETLKECVQPGQKPMLIPM
                     PDYRPKYPKINPEVEINPNHPNLTIWHNKIDCCMFIGVHCHQANLSLKIIRGGTSCYT
                     VAMCAQAGHEDAMLSFRDASVEKILKLGEWIRKLKGTVKPRLTSAKTSASN"
     gene            727..1938
                     /locus_tag="PLS35_20395"
     CDS             727..1938
                     /locus_tag="PLS35_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNI21087.1"
                     /translation="MAEIKSHIGTQNKKGQTYTDPWKMMHEAPRNPSFYTGSEVIKEA
                     LRRASCDVMIAYPITPQSEAAALIGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTIEISYLLNTGML
                     VWHAETAQDFFDWILKGFMVSEEPEVHLPLALCCDGFFVTHTKDVVNLTPADMCLPPY
                     DPYRSPVPCMDMECPPVRMMRDPFIMKSNYISYATHASWQQEIWAAIERSRKHTIKWI
                     NGLVDTENTDAEVVIVASGTAVSQGREAIRLLEDEGVRCGLVKVKSLRPWPGEEIREA
                     TKNAKHIFVPEFNVTGWLAKEIKATIPNSERVHAGPHVCGGMTMPPEIIVSEIKTALG
                     LRSESLAGRGS"
BASE COUNT          530 a          549 c          534 g          454 t
ORIGIN      
        1 ggtgtccctt ctccaagttt gttaacccca aaaagagagc aggttcgaat gactcagtat
       61 cgcgtgttac caggcccaga acacttcctg ccaccggccg cggcttcgat gggaatctat
      121 cttcccaatc caggcgaggc acatatcaac ggcgtcatcg ttccggaaga aaaagcctac
      181 gaggaagccg cacgccaatt tctcatggct caggtgccaa ccattttccc aggtcctctc
      241 gttctctggg cctggaatga aaaggcggcg aagaaggctg ccgctgtccg aagtctttac
      301 gaaactctga aagagtgcgt gcagccaggg caaaagccga tgctgattcc aatgccggac
      361 tatcgcccca aatacccgaa gattaatcca gaagtcgaaa tcaatccgaa ccatcctaat
      421 ctcaccatct ggcacaacaa gatcgactgc tgcatgttca tcggcgtgca ttgccaccaa
      481 gcgaatttat ccctcaagat tatacgcggc ggaacgtcgt gctataccgt cgcgatgtgc
      541 gcacaagcag gacatgaaga cgccatgctc tccttcagag atgcgtcagt agaaaaaatt
      601 ctgaagttgg gcgagtggat tcgcaaactg aaaggcaccg ttaaaccacg gctgacatca
      661 gctaagacga gcgcttccaa ctagcgagtt tggagcactt ctaacgattg aaaggaggcc
      721 gagtccatgg cggaaataaa atcccatatc gggacacaga ataaaaaggg ccagacctac
      781 acagatccgt ggaaaatgat gcatgaagct cctcgcaatc cgtcctttta tactggtagc
      841 gaggtcatta aagaggcgtt gagacgggct agctgcgatg tgatgattgc ttatcccatt
      901 actccgcaga gtgaagcggc agcccttatc ggggagttgt tcgcagaagg ttatatcggt
      961 gattacttcc gcggagagag tgagttcgcc gttatgtccc aatgtgccgg cgcagcattc
     1021 ggaggagcgc gagtcttcac aacgacagcg ggacccggaa cgatgcgtgc catggaaaac
     1081 tttcccatgt gggctggtgc gcgcttacca attcaaatga tcgtcacctg ccgaggcatc
     1141 aattcgccac tgtcgattca gccagacacc attgaaatct cctacctctt aaacaccggc
     1201 atgctcgttt ggcatgcaga aacggcgcaa gatttctttg actggatcct caaaggcttc
     1261 atggtatccg aagagccgga agtgcatttg ccgttagcct tgtgttgcga tggtttcttt
     1321 gtgacccaca ccaaggacgt cgtcaatttg accccggctg acatgtgcct tcctccctac
     1381 gacccctacc gttcccccgt gccgtgcatg gatatggagt gccctcctgt ccgtatgatg
     1441 cgcgacccct tcattatgaa gagcaattac atcagctatg ccactcatgc ttcttggcaa
     1501 caagagattt gggcagcgat cgagcggtca cggaaacata cgatcaagtg gatcaatggt
     1561 ctggtcgata ctgaaaatac cgacgcagag gtcgtcattg ttgcctcggg taccgctgtg
     1621 tcccaaggac gagaagccat ccgcctgctt gaggacgaag gtgtacgttg cgggttggtc
     1681 aaggtgaaat cacttcgtcc atggcccgga gaagagattc gcgaagcgac gaaaaatgca
     1741 aaacatattt tcgtgcccga gttcaatgtg acgggatggc tggcaaagga aattaaggcc
     1801 acgattccca acagcgaacg cgtccatgcc ggtccgcacg tctgcggcgg tatgaccatg
     1861 ccaccggaaa ttattgtctc cgagattaag acagccctcg gcttgcgctc tgagtcgctt
     1921 gccggaagag gcagctgata tatcgttccc tgtcgttaca cagtgacctg agccatcaaa
     1981 ctcgagatcg ataagccttg aggaggcata catgagcaaa gagcgaatca aaatttcgcc
     2041 ggatctatat gacatcatgc catcgga
//
LOCUS       DAOQEW010000389         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_32645_length_2055_cov_8.724500, whole genome shotgun sequence.
ACCESSION   DAOQEW010000389 DAOQEW010000000
VERSION     DAOQEW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32645_length_2055_cov_8.724500"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /gene="ndhC"
                     /locus_tag="PLS35_20400"
     CDS             complement(<1..338)
                     /gene="ndhC"
                     /locus_tag="PLS35_20400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNI21088.1"
                     /translation="MGNEAAPLNYIPILIFILIAFAFGAVQILLGRIVRPSRPYRAKL
                     APYESGSPLFSDAKIQFPIRYYIIAMLFVIFDIEIVFMFPWAVAFKKLGLVGLVEMVV
                     FIGILIVGFW"
     gene            complement(414..1589)
                     /locus_tag="PLS35_20405"
     CDS             complement(414..1589)
                     /locus_tag="PLS35_20405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNI21089.1"
                     /translation="MSTGHPQLLAEIRAEIAASGPIPFARFMELALYHPQYGYYVRPV
                     EDPTQERIGWSGDFYTSSDVHPILGQALAKQAQQLDACLGHPNPFTVVEMGPGKGLLA
                     RDFLTACQNAPGNLSERLRYILIERSASMRALQHQHLAPWIGRAGHVTWLDRLEDLPP
                     DSVTGLFFSNELVDAFPVHRLAVVDGHPQEIYVDVHEEGFTEIHRPLSEELSAYLQNG
                     GIRLPDGYRTEINLKAVQWIKQVAQIMARGLVMTIDYGHTAEDLYGPERKNGTFLCYY
                     SQTTSEDAYERVGQQDMTAHVDFTTLAQAGQAAGLELTGFTNQMSFLIGLGAEQFLES
                     LQPESAEFYAAIHLLRPEGMGRTFKVLVQHTGMEKPELDGLRFKPFFGSILAARSAA"
     gene            complement(1804..>2055)
                     /locus_tag="PLS35_20410"
     CDS             complement(1804..>2055)
                     /locus_tag="PLS35_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNI21090.1"
                     /translation="FDMFSSMMERIKSDVLERMFRVQAVRGEQPPPPAPEPIPPPRMV
                     LNRSDEPATQTVQSQGDKTGRNDPCPCGSGKKFKKCHGA"
BASE COUNT          441 a          591 c          595 g          428 t
ORIGIN      
        1 taccagaacc cgacgatcag gatgccgata aacaccacca tctccactaa ccctaccaac
       61 cccaacttct tgaatgccac ggcccaggga aacatgaaca cgatttcgat atcaaagatc
      121 acgaacagca tcgcgatgat gtaatagcga atcggaaatt gtattttcgc gtccgagaac
      181 aacgggctgc cgctttcata cggcgcgagc ttggcgcgat aaggccggct cggccggacg
      241 atacgtccca acagaatctg cacagcgccg aacgcaaagg cgatcaggat aaaaatcagg
      301 atcgggatgt aattcagcgg tgcggcttcg ttacccatgg tttgtccggg aatcgtgaaa
      361 cgaaaaccct ctcacggcgg tcgtcacaat tagatggcgt cctgccggtt cctttacgcg
      421 gcggaacgag ccgccaaaat cgatccgaag aacggcttga atctcaaccc gtccagttcg
      481 ggcttctcca tgcccgtatg ttgcaccagc actttgaacg tccgccccat tccttccggt
      541 cgcaggaggt ggatggcggc ataaaactcc gccgattccg gctgcagcga ctccaggaac
      601 tgttcggcgc ccaggccgat cagaaaactc atttgattcg taaagccggt caactcaagt
      661 ccggccgcct ggcctgcttg cgccagggtg gtgaagtcca catgcgccgt catgtcctgc
      721 tggccgaccc gctcgtacgc gtcttccgag gtggtctgac tgtagtagca gagaaatgtg
      781 ccgttcttgc gctccggtcc gtagaggtct tcggccgtgt gcccgtagtc gatcgtcatc
      841 accaggccgc gtgccatgat ttgcgcgacc tgtttgatcc attgaacggc cttgagattg
      901 atctcggttc ggtatccatc gggcagccgg atgccaccgt tttgcaaata ggccgacagc
      961 tcttccgaaa ggggacgatg gatttccgtg aagccctctt cgtgaacatc gacataaatc
     1021 tcttgcggat ggccatccac caccgccagg cgatgcaccg ggaaagcatc caccaattcg
     1081 ttggaaaaga agaggccggt gacactgtcc ggcggaagat cttccaggcg atcaagccag
     1141 gtcacgtgac cggctcggcc gatccatggc gcgagatgct gatgctggag ggcgcgcatt
     1201 gaggcgctgc gttcaatcag gatgtaacgg agtcgttcgc tgagattgcc gggcgcgttc
     1261 tggcaggcgg tgagaaaatc ccgagccaac aatcctttgc ccggccccat ctccaccacg
     1321 gtgaacgggt ttggatgtcc caggcaggca tcgagttgct gcgcctgttt ggccaatgcc
     1381 tggccaagaa tcggatgcac atcggaactc gtatagaaat caccggacca tccaatacgt
     1441 tcctgggtcg gatcttcgac aggacgaacg taatagccgt attgcggatg atacagggcc
     1501 agctccatga aacgggcgaa gggaataggt cccgacgctg cgatttcagc cctgatttcc
     1561 gccaggagtt gtggatgccc agtgctcaaa gtgacaccac ccttgtcggt agtcgactgc
     1621 cgtttcaggc gccatcgcaa gaaaccgctc ttctaccgga ttctctaggg aaaagagggg
     1681 gttagattag ccgctggctc agcggcaagt caaggcgaaa agacccggaa agacgcgagt
     1741 ctctcgacat ttgcggaccg tttgtttctg ttcactccgc ggaggcgggc caggaattcc
     1801 cggctatgct ccgtgacact tcttgaattt ttttccgctt ccgcacgggc agggatcgtt
     1861 gcggccggtc ttgtcgccct ggctttggac ggtctgagtg gccggttcgt cgctgcggtt
     1921 caggaccatg cgcggcggag ggatcggttc gggtgccgga ggaggcggtt gttcgccccg
     1981 cacggcttgc acgcggaaca tgcgctccag cacatcggac ttgatgcgct ccatcatgga
     2041 ggagaacatg tcgaa
//
LOCUS       DAOQEW010000390         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_33415_length_2031_cov_8.328947, whole genome shotgun sequence.
ACCESSION   DAOQEW010000390 DAOQEW010000000
VERSION     DAOQEW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33415_length_2031_cov_8.328947"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..1840
                     /locus_tag="PLS35_20415"
     CDS             518..1840
                     /locus_tag="PLS35_20415"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNI21091.1"
                     /translation="MANLVIIGSQWGDEGKGKIVDILAKDADMVVRYQGGSNAGHTVI
                     NKRDTFIFHLIPSGILYRGTRCLIGNGVVVDPGALIEEMDHLQGQGVKIGKNFAVSDR
                     AHLILPYHKAIDKASEQSKGVRRIGTTGRGIGPSYGDKMARIGIRMGDLLNPPLFKQK
                     LEENLVDINWLLEQLHKVDCFELEKVYQQYMAYADRLKSYILDTTMVVNKAVDAGKTV
                     LFEGAQGTHLDVDFGTYPYVTSSSSSAGGACTGTGVGPTKIEAVMGITKAYTTRVGSG
                     PFPTELTDEVGGWLQERGKEFGATTGRARRCGWFDSVVVRHATRVNGLSSLAVTKLDV
                     LDGRNELKICTGYRVNGKLHRDMPSDLLALTNCEPVYERIRGWSAPTTGVTTYKKLPV
                     EAKRYLTRIEELAECRIDMISTGSRRDETIILNNPLKAGRRKRAPRSR"
     gene            1956..2031
                     /locus_tag="PLS35_20420"
     tRNA            1956..2031
                     /locus_tag="PLS35_20420"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1989..1991,aa:Lys,seq:ttt)
BASE COUNT          464 a          545 c          608 g          414 t
ORIGIN      
        1 tcgtgaatgc cattcacgcg cgctcccaac tgcgccacag ccccacgcaa gcttggatct
       61 tatgtgcaca ggcagcgaga aaacgactgc tgctgcagac gtaactccgc aatctagcac
      121 gggcggattt tgtcagtcaa gaaaagggtg gtgaggcggc gatgtgccgc gcccttttgt
      181 gctcgcgcaa cgcgcggcct cagaaggccc tcgttggacg cgcgcaatgt aaagggcgcg
      241 gtacatgccg ccttgtgaga gcaaggaagc ggcggagaag atccaggatc atccgccgtc
      301 taagagaatg cggcgtcggg aggaagaata cccagcctat ccgtcttagg aagcacgctg
      361 gaggtgacgc aaccaagggc ggaagcttgt tctcgaaaac cctgcttgac aaaccgcggt
      421 gtgaatccta ccatgatgcc cttccggctc tgatcagtac gaggtcagag cctttttttc
      481 gagttcagac gggaagaaag agcagaggca gcgaacaatg gcgaatctcg tcatcatcgg
      541 ctcccaatgg ggcgacgaag gtaaagggaa aattgtcgat atcctggcca aggatgccga
      601 catggtcgtg cggtaccagg gcggctccaa tgccggccat acggtcatca ataaacggga
      661 cacgtttatt ttccatctca ttccttcggg catcttatat cgtggcaccc ggtgcctgat
      721 cggtaacggc gtcgtggttg atcccggagc attgatcgaa gaaatggatc atctccaagg
      781 ccagggcgtc aaaatcggaa aaaatttcgc cgtcagcgat cgtgcccatt tgatcctgcc
      841 gtaccataag gccatcgata aagcctcgga gcagtctaaa ggtgtccgtc gtattggaac
      901 cactgggcgt gggatcggcc cgtcctacgg cgacaaaatg gcgcggatcg gcattcgcat
      961 gggcgatttg ctgaatcccc cgctcttcaa gcagaaactg gaagaaaatc tcgtcgacat
     1021 caattggctc cttgagcagc tgcacaaagt ggactgcttc gagttggaaa aggtctacca
     1081 gcaatacatg gcctatgctg atcgactgaa gagctacatc ctcgatacga cgatggtcgt
     1141 caataaagcc gtcgacgctg gaaaaacggt cctgttcgaa ggcgcgcagg gaacgcactt
     1201 ggacgtggac ttcggcacct atccctacgt cacttcgtcg agttcttcgg ccggcggcgc
     1261 ctgtacggga accggggtgg ggccgacgaa aatcgaggcg gtgatgggca ttaccaaggc
     1321 ctatacgacg cgcgttggca gcggaccttt cccgaccgaa ttaaccgatg aagttggcgg
     1381 gtggctccag gagcgcggaa aagaattcgg tgccacgacc ggccgggctc gccgctgcgg
     1441 gtggtttgac agtgtcgtgg tccgacatgc gacgagagtc aacgggctgt cttctctcgc
     1501 ggtcacgaaa ttggatgtgt tggacggccg gaacgagttg aagatctgca cgggctaccg
     1561 ggtgaacggc aagcttcatc gtgacatgcc gtcggacttg ctcgcattga ccaattgcga
     1621 gccggtgtat gaacgcattc gtgggtggag cgcacccaca accggtgtta ccacgtataa
     1681 gaaattgccg gtggaagcga agcggtatct cacgaggatc gaagaactgg ccgagtgtag
     1741 aattgatatg atctctaccg gttcacggcg tgatgaaacc atcattctca acaatccgct
     1801 caaggcaggc cgccgcaagc gagcgccacg ttcacgatga tcgctgtgtt ttttgcgtct
     1861 tccccgatgt gcgccgtagc gatacaggac gcgtcagtgg taagatccct cccgtcacga
     1921 tcctcgcgtg acgatgtgtc gtctccatct tgggagagtc ggtagctcag ctggtagagc
     1981 atcgcccttt taaggcgagg gtcctgggtt cgaatcccag ccgactcacc a
//
LOCUS       DAOQEW010000391         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_33957_length_2014_cov_6.926493, whole genome shotgun sequence.
ACCESSION   DAOQEW010000391 DAOQEW010000000
VERSION     DAOQEW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33957_length_2014_cov_6.926493"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLS35_20425"
     CDS             complement(<1..411)
                     /locus_tag="PLS35_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21092.1"
                     /translation="MLNDQLVISSVAGPFREPREPVFSYDYSIQRATWAATHAVRVKI
                     ALAEELDYVKTKLLGTVTGSPGQQLMLNKLLSRKIGDQKLRIAEEEGWLKERADVLVP
                     PFTGPLAHHFAQLDSWVQNQQETLRSEIKQTIGLA"
     gene            complement(440..>2014)
                     /gene="hemG"
                     /locus_tag="PLS35_20430"
     CDS             complement(440..>2014)
                     /gene="hemG"
                     /locus_tag="PLS35_20430"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HNI21093.1"
                     /translation="TGANTHAERLGFTDRHPHVGRGGTRVLARSLAAPPVARTSRSVV
                     IIGGGISGLSTAFALLEQAAAADVPLSCTILDAAPVWGGKIVTHRVGRLVMEAGPDSF
                     LSQKPWAMDLCRRLEIADELINTNPVEKKASVLRGGQLHELPEGLVTFTPTQLGPFFR
                     SGLLSWVDLARMGCDVLIPPRRSTEDESLASFFRRRFGQHACERVMEPLMAGIYAGDA
                     EQMSLRATFPRFYELEQTHGSVIRGMMAARRARAQKASDGRPRHTMFVTLKNGLADLV
                     AGLTTRIRETGGVLKSGVQAEALRVRSHQAGRWMYDVICTDGTALSAEALVLATPAYV
                     SADLVRPLSPLAAGLMETIPYASTATISLIYPAAAVGTRLQGFGFVVPRVEGRDLIAA
                     TWTSIKWPHRAPPEEVSVRCYLGGVGREAILQQDDEALVRCVREELASIVGLQATPHY
                     VEVNRWHRAMPQYTLGHLDRLTQLDAALSRFGGLAVTGAGYRGVGLPDCIRDGAEAAA
                     QTLRYLQTASKREAES"
BASE COUNT          413 a          655 c          576 g          370 t
ORIGIN      
        1 cgccaaaccg atcgtctgtt tgatttcgct tcgcaaggtc tcctgctgat tctggaccca
       61 agaatcgagc tgcgcaaaat gatgcgccag cggtccggtg aagggaggca ccaggacatc
      121 ggcgcgctcc ttcaaccacc cttcctcctc ggcgatgcgg agcttttggt cgccgatctt
      181 gcgcgacagc agcttattca gcatcaactg ctggcccggg ctgccggtca cggttcccag
      241 gagcttcgtt ttcacatagt ccagctcttc tgccagcgcg atcttcacgc gaaccgcatg
      301 agtggccgcc caggtcgccc gttggatgga atagtcatac gaaaacaccg gctcccgggg
      361 ttcgcgaaac ggccccgcta cactggagat caccaactgg tcatttaaca tcgtccatgt
      421 ccttctgttg cttcgtctct cacgattccg cctctcgttt ggaggctgtt tggaggtagc
      481 gtaacgtctg tgccgccgct tctgcgccgt ctctgatgca atcgggcaaa ccgacgccgc
      541 gatatcccgc gccggtgacg gccaatccgc cgaaacgtga aagcgcagca tcgagctgtg
      601 tcagtcgatc gagatgcccg agcgtatatt gcggcatcgc ccgatgccac cggttgacct
      661 cgacataatg cggtgtcgcc tgcagaccga cgatagacgc caattcttcg cgcacgcaac
      721 ggaccaatgc ctcatcgtcc tgctgcagga tggcctcgcg tcccacccca ccgagatagc
      781 agcgcacgga cacctcttcg ggaggcgctc ggtgcggcca cttgatggac gtccaggtcg
      841 cggcaatcag gtcacggccc tcaacccgag gaaccacgaa accaaagcct tgcaagcggg
      901 ttcccaccgc agcggcgggg tagatcaagg aaatggtggc cgtcgaagcg tagggaatcg
      961 tctccatcaa tccagccgcc agcgggctca gcggccgaac caggtcggcg ctcacatagg
     1021 ccggtgtcgc gagcactaac gcttccgcgg acagcgccgt cccatcggtg cagatcacat
     1081 cgtacatcca gcgtcccgcc tggtgcgagc gtacccgaag cgcttccgct tgcacacccg
     1141 acttcaagac gccgccagtc tcccggatac gcgtggtcag tccggcgacg agatccgcga
     1201 ggccgttttt cagcgtgaca aacatggtat gccgaggacg cccatcggag gccttttgcg
     1261 ccctggctcg acgagccgcc atcatgcccc gaatcacgct gccatgtgtc tgctcgagct
     1321 cataaaaccg gggaaacgtc gcgcgcaggc tcatctgctc ggcatcaccc gcgtagattc
     1381 ccgccatcag cggttccatg acccgctcgc aggcatgctg tccgaatcgg cgacggaaaa
     1441 aggaggcgag cgattcatcc tccgtggacc gacgcggggg aatcagcaca tcacaaccca
     1501 tccgggccag gtcgacccac gacagcaatc cgctgcgaaa aaacggacct aattgagtcg
     1561 gcgtaaaagt cacgagccct tcgggcaact cgtgcaactg cccgcctctc aatacgctgg
     1621 cctttttttc aacagggttc gtattgataa gctcatcggc gatttcgagc cgccgacaga
     1681 ggtccattgc ccagggcttc tgagacagga aggaatccgg acctgcctcc ataaccaacc
     1741 ggccgacacg atgggtgacg atctttccgc cccagacagg cgcggcatcg aggatggtac
     1801 aggacagcgg cacatcggcc gctgcggctt gctccaggag agcaaaggcc gtggagaggc
     1861 ctgagatgcc tccaccaata atgaccactg accgtgaagt gcgcgccaca ggtggtgccg
     1921 ctaatgaacg cgcgaggact cgtgtgcctc cacgaccgac atgaggatgt cgatcagtga
     1981 agccgaggcg ttcagcatgg gtattcgctc cagt
//
LOCUS       DAOQEW010000392         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_34160_length_2007_cov_5.788934, whole genome shotgun sequence.
ACCESSION   DAOQEW010000392 DAOQEW010000000
VERSION     DAOQEW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34160_length_2007_cov_5.788934"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PLS35_20435"
     CDS             complement(<1..1117)
                     /locus_tag="PLS35_20435"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HNI21094.1"
                     /translation="MTIDSRHCQDLDAALNLEWLETNGRGGYASGTVAGANTRRYHAM
                     LLIARHPPVDRVVLVNHVEEFVESGGRTFPLSTNLYHDAVHPEGYQYCEEFSAYPWPT
                     WRYAGHGIRLIRELLCPQGRDLVILRWRLLDDASSPVVLRVRPMLSGRDFHAAQRENA
                     AVRSEATTEEGQVMWHPYEGLPAVRALHNGQYHHGPDWYRHVHYRVERERGLDHIEDW
                     WSPGECVFTITPGTAAELVFTSEPLPSYDVTQAIEAERLRRGQLQQSIPTEDVLTRRL
                     WAATGAYLVQRGARQTVVAGYPWFADWGRDTFVALPGLCLVTGRYDVARQVIEAFASY
                     VSQGMVPNRFPDIGEQPEYNTIDASLWFVHAVDRYLHY"
     gene            complement(1132..>2007)
                     /locus_tag="PLS35_20440"
     CDS             complement(1132..>2007)
                     /locus_tag="PLS35_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HNI21095.1"
                     /translation="SKFFEHFVYICRAMNNIGNAKIELWDREDGFFYDVLHLPDGKTF
                     PMKVRSMVGLIPLFAVETLDSELIDQLPRFKHRMQWFIENRPDFSLHIETQSQDGEVR
                     RFLSLVNRRRLAKVLRYLLDEQEFLSPHGVRALSRYHKDHPYVFSMMGTSHCVEYQPA
                     ESSNGLFGGNSNWRGPVWFPVNYLLIESLQKFHYYLGESFRVEYPTGSGQRRNLAEVA
                     AELSRRLTHTFLRGPDGRRPVYGGTAKFQQDPHWRDLLLFYEYFHGDNGAGLGASHQT
                     GWTGLVAKLIQQSGE"
BASE COUNT          429 a          642 c          556 g          380 t
ORIGIN      
        1 tgtagtgcag atagcgatcc accgcgtgaa cgaaccacaa ggaggcatcg atcgtgttgt
       61 attcaggttg ttcaccaata tcgggaaacc ggtttggcac cattccctgt gagacgtagg
      121 aggcgaatgc ctcgatcacc tgccgcgcca cgtcgtaccg tcctgtcacc agacacagcc
      181 ccggcaacgc cacgaatgtg tcccgccccc aatcggcaaa ccagggatat ccggccacca
      241 ccgtctgccg ggctccgcgc tgcacgagat aggcgcctgt cgcggcccac aaacgtcgcg
      301 tcaggacatc ctcggtcggg atggactgct gaagctgtcc gcggcggagg cgctcagcct
      361 cgatcgcctg cgtcacgtcg taggacggca acggctcgct ggtgaagacc agttccgccg
      421 cagttcctgg cgtgatggtg aacacacatt ctcccggcga ccaccagtct tcgatatgat
      481 ccaggcctcg ctcccgttcg acgcgatagt ggacatggcg ataccaatcc gggccgtgat
      541 ggtactggcc gttgtgcaac gctcgtaccg ccggcagccc ttcataggga tgccacatga
      601 cctgaccttc ctctgtcgtc gcctcactcc gaacggcggc gttttccctc tgtgccgcat
      661 gaaaatctcg tcccgacagc atcggccgga cccgcaacac caccggtgat gaagcatcgt
      721 ccagcagccg ccaccgcagg atcacgagat cgcgcccctg cggacacaac agttcccgta
      781 tcagacgaat accgtgcccg gcataacgcc acgtcggcca gggataggca gaaaactctt
      841 cgcaatactg gtatccctcc ggatgcaccg cgtcgtgata gagattggtc gacaacggga
      901 acgtccgccc gccgctttcg acgaattctt caacgtgatt gaccagcacg acgcgatcga
      961 ccggcggatg tctggcgatc aaaagcatgg catggtagcg ccgcgtattg gcaccagcga
     1021 ccgtgccgga cgcatatcct ccgcggccat tcgtctccag ccactccaga ttcaacgctg
     1081 cgtccaggtc ctggcaatgt ctcgaatcga tcgtcatgac ggatctccgg cctattcgcc
     1141 tgactgctga atcaatttgg ccaccagacc ggtccagcct gtctggtgac tcgcgccgag
     1201 gccggcgccg ttgtcgccgt ggaagtattc gtagaacaac aggagatccc gccagtgcgg
     1261 atcctgctga aacttcgccg tcccgccata caccggccgc cgaccatccg gtccccgtaa
     1321 aaatgtatgc gtgaggcggc gggacaactc ggcagccacc tccgccagat tgcgcctctg
     1381 ccccgaccca gtcgggtact caacgcgaaa tgattcgccc agataataat gaaacttctg
     1441 cagcgattcg atcagcagat aattcacggg aaaccagacc ggaccgcgcc agttcgagtt
     1501 cccgccgaac aggccgttgg aggattccgc cggttgatat tcgacgcagt ggctggtgcc
     1561 catcatcgaa aacacgtacg gatggtcctt gtgataccgg gatagggcac gcacgccatg
     1621 aggcgacaaa aattcctgtt cgtcgagcaa ataccggagc accttggcca accgtcgccg
     1681 attcaccagc gagaggaacc ggcgcacctc accgtcctgc gattgggttt cgatgtgcaa
     1741 gctgaaatca ggccggttct cgatgaacca ttgcatgcga tgcttgaagc gcggcaactg
     1801 gtcgatgagt tcggaatcca atgtctccac ggcaaataga gggatgagtc ccaccatgga
     1861 ccggaccttc atggggaagg tcttgccgtc cgggagatgc agcacgtcgt aaaaaaaccc
     1921 gtcttccctg tcccagagct cgatcttggc gttgccgatg ttgttcatcg cccggcagat
     1981 gtagacgaaa tgctcgaaga acttact
//
LOCUS       DAOQEW010000393         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_34251_length_2004_cov_6.477681, whole genome shotgun sequence.
ACCESSION   DAOQEW010000393 DAOQEW010000000
VERSION     DAOQEW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34251_length_2004_cov_6.477681"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2004
                     /gene="pglX"
                     /locus_tag="PLS35_20445"
     CDS             <1..>2004
                     /gene="pglX"
                     /locus_tag="PLS35_20445"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HNI21096.1"
                     /translation="RLRDIPRLILEKNLYGLDIDDRAAQLAGFALLMKARADDRRLFN
                     DPPRLNVLSLQESKGLELGELATHLAPFGVQRATIKALLDTFEHAKTFGSLIQIPDAL
                     NAQLPALAEAVGKANETGDLYAQAAAQDLLPLVEQLKVLGRKYDAVVANPPYMGMKAM
                     NAQLKELASVEYADFKIDTYSMFVCRALSFVNTTGIASLVTMHSWMFLHRFENMRQRL
                     LTEATIQCLGHLGARAFSAISGEVVQTCCFALGRQHVADYRPVFSRCVDGDETYKKEA
                     LKSPASRYARFILEDFEPISGRILAYWISCDVMRHFQGERKVADHANPRHGMVTSDND
                     RFLRFHWEVSFANQAHGCQSREAARETLKKWFPYNKGGAARRWYGNAEYLLNWANDGF
                     EVIEYARELYGSASRKIQSQEFYFQDGLTWSALGSGDISLRANGPGYLFDSKGSSMFF
                     ASDDVKYLMCGLFNSNVAALFISVMSPTLDFSAGSMANMPLAEQIEIRVGKQVRECSM
                     KAIGIARADWDAFERSWDFQSLPLLTAPSDPTPTLESRYASWITQNRDTIAEMKRLEE
                     ENNRIFIDAYGLQHELTPDVPIEQITLTVNPAYRYGGDFTEEEQWTRFRRDSMEELVS
                     YVIGCMMGRYSLDEPGLIYAHAGNVGFDPTRYKTFPADADGIV"
BASE COUNT          441 a          572 c          568 g          423 t
ORIGIN      
        1 accgcctgcg cgacatcccg cgcctgatcc tggaaaagaa cctctacggc ctcgatatcg
       61 atgaccgcgc cgcgcaactc gccggcttcg ccctgttgat gaaggcccgc gccgacgacc
      121 gccgcctgtt caacgaccca ccccggctca acgtgctgag cctgcaggag agcaaggggc
      181 tggagctggg cgaactcgcc acccacctcg cgcccttcgg cgtgcagcgc gccaccatca
      241 aggcgttgct cgacaccttc gagcatgcga agaccttcgg ctcgctgatc cagattccgg
      301 acgcgctgaa tgcgcaactg ccggcgctgg cggaggcggt ggggaaggcg aacgagactg
      361 gggacttgta cgcacaggcg gcggcgcagg atttgctgcc gctggtggag cagttgaagg
      421 ttctggggcg gaagtacgat gcagtggtgg cgaatccgcc gtatatgggt atgaaggcaa
      481 tgaacgctca actcaaggag ctcgcctcag ttgagtatgc ggacttcaag attgacacat
      541 attcaatgtt cgtttgccgt gctctgagct tcgtaaacac gacagggatt gcttcgctgg
      601 tcaccatgca cagttggatg tttctccacc gtttcgagaa tatgcgccag cgccttctca
      661 ctgaagcgac cattcagtgc cttggtcacc tcggagccag ggcattctct gccatttcgg
      721 gggaggttgt tcagacgtgt tgcttcgctc ttggtcgcca acacgtcgcc gattatcgac
      781 cggtcttctc aaggtgcgta gatggtgatg agacctacaa gaaggaagca ttgaaatcgc
      841 cagcatctag atacgctcga tttatcttgg aggattttga gcctattagc ggaaggattt
      901 tggcttactg gattagttgc gatgtcatgc gccattttca aggagaaagg aaggtcgccg
      961 accacgccaa tcctcgccat ggaatggtaa caagtgacaa cgaccggttc ttacgcttcc
     1021 attgggaagt gtcatttgcg aatcaagccc atggctgcca atcacgagag gctgccagag
     1081 aaacgctcaa gaaatggttt ccctataaca aaggaggggc tgccagaagg tggtacggca
     1141 atgccgagta ccttctcaac tgggcgaatg acggatttga ggttattgag tatgcacgag
     1201 aactctatgg gtcggccagt cggaagatcc agagtcaaga gttctatttc caagatggcc
     1261 taacttggtc tgctttggga agcggggata ttagtcttcg agctaacggg cctggctatc
     1321 ttttcgactc aaagggcagt tcgatgttct tcgcgtcaga cgacgtgaaa tatctcatgt
     1381 gtggattgtt caactcgaac gtagcagctt tgttcatctc cgtcatgtca cctacgctcg
     1441 acttcagcgc aggttcaatg gctaacatgc cccttgccga gcagatcgag attagggttg
     1501 gcaaacaagt acgtgaatgc agcatgaagg caatcgggat cgcgagagcc gattgggacg
     1561 catttgaacg gtcgtgggac ttccaatctc tcccgctcct gacggccccc tctgacccca
     1621 cgcccaccct cgaatcccgt tacgcctctt ggatcaccca gaaccgggac accatcgccg
     1681 agatgaagcg tcttgaagag gagaacaacc gcatcttcat cgacgcctac ggtctgcagc
     1741 atgaactcac tccggacgtc cccatcgagc aaattacgct cacggtgaac cccgcgtacc
     1801 gctacggcgg cgacttcacc gaagaggagc aatggacgcg cttccgccgg gacagtatgg
     1861 aggagctagt ctcctacgtc atcggctgca tgatgggccg ctacagcctc gacgaacccg
     1921 gcctgatcta cgcccacgcc ggcaacgtag gcttcgaccc gacccgctac aagaccttcc
     1981 cggccgacgc cgacggcatc gtgc
//
LOCUS       DAOQEW010000394         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_34253_length_2004_cov_5.799384, whole genome shotgun sequence.
ACCESSION   DAOQEW010000394 DAOQEW010000000
VERSION     DAOQEW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34253_length_2004_cov_5.799384"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="PLS35_20450"
     CDS             <1..679
                     /locus_tag="PLS35_20450"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNI21097.1"
                     /translation="LAMRIVGNMGAPLLGVLGGLVSSTATTLAYARHAQRSANLAPLS
                     TFVILMANVIVPLRLGVITSTVSPQMMATLWPVLIGNFVAGGVAVFLLWREIGQGEQL
                     PLPAVTNPTEIRAALTFGTMYAAILLLASWLSDLAGNRGLYLVSFISGLTELDAIVLS
                     TLRLFETQKLEAVQAARAIGIAILSNLVFKTALLAWIAGHGFLRRCLPGMAGIGLGVG
                     IGLWFVK"
     gene            676..1230
                     /locus_tag="PLS35_20455"
     CDS             676..1230
                     /locus_tag="PLS35_20455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNI21098.1"
                     /translation="MRKSDKETEPEKRSKMMRSGEAFREDSELQMRENRIAMPLDAFC
                     CDFIVNNIELADVLPSHTHGQGLFASRDIEAGVVLAVLDGQIVEYDFYHQVKRAVQLS
                     ERVNGYLFMEWNCLGEGRILARMFRTKYSFINHSRNPNLVLTGLPLEVRTARAIHAGE
                     ELFLDYSAEPLPESYLCGPGAAYL"
     gene            1319..1816
                     /locus_tag="PLS35_20460"
     CDS             1319..1816
                     /locus_tag="PLS35_20460"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNI21099.1"
                     /translation="MIRHPPSICQDPVCGMDVIIAESRYPAIYHQMTFHFCSAQCRDH
                     FLKAPALYAGAVRREDVKAMPKRRVLRITTGAALDETCRRLQAMMGVSAIRAEADRLH
                     IDYDLRQVSLSQIEAEIEAAGVILKGGLHALRRSLWRFTEHNELQNAARTGPQACCNR
                     PPTPR"
BASE COUNT          417 a          512 c          578 g          497 t
ORIGIN      
        1 tcttgcaatg cggattgttg gaaatatggg ggcccccttg ctgggtgtcc tcggtggact
       61 tgtttcgagt accgcgacaa cacttgcgta tgcgcgccat gcgcagcggt ccgccaactt
      121 ggctccacta tcaacattcg tcattttaat ggccaacgtg atcgtgcctt tacgcctcgg
      181 agtgattact tcaaccgtat cgcctcagat gatggcgacg ctttggccgg tgttgatcgg
      241 gaatttcgtg gcaggagggg tggcggtgtt tcttctatgg cgcgagattg gacagggcga
      301 gcaattaccg ctacctgcgg taacgaatcc caccgaaatt agagctgcac tcacattcgg
      361 cacgatgtac gcagcgatcc tgctgctggc gtcctggctt tcagatctgg ctgggaatcg
      421 agggctctat ctcgtttcat tcatctctgg tttaactgaa ctggatgcca tcgttctttc
      481 cacacttcga ttgtttgaaa cacagaaact ggaggccgta caggcagctc gcgccatagg
      541 tatcgcgata ctatcgaatc tcgtgtttaa aaccgcgctt ttggcttgga ttgcgggaca
      601 tggctttctg cgacgctgct tgccgggaat ggcgggaatt ggtctgggtg taggcatagg
      661 gctctggttt gtgaaatgag aaagagcgac aaagagacag agcccgaaaa acgttcaaag
      721 atgatgcgct caggggaagc cttccgcgaa gattcagaac ttcaaatgcg ggaaaatagg
      781 atcgcaatgc cgctcgatgc gttttgctgc gacttcattg tcaataatat cgaactagct
      841 gatgttctgc cgagccatac tcatgggcaa ggactgtttg cttcaagaga tattgaggca
      901 ggagtcgtgc tggcagtgct ggatggacag attgtggaat atgatttcta tcatcaggtc
      961 aagcgagccg ttcagctatc agagagggtt aatggctatc tatttatgga gtggaactgc
     1021 cttggagagg ggcgaatatt ggcgagaatg tttcgcacaa agtacagctt cattaatcat
     1081 tccagaaatc cgaatttggt gctgaccgga cttcctcttg aggtcaggac ggcaagggcg
     1141 attcatgccg gagaggaact ttttctagac tacagcgctg agccattacc cgaaagctat
     1201 ctgtgcggtc ccggtgcggc gtatctttag agttgtgcat cttgtctaca aatgcctctt
     1261 tgggttcttg ttgcaggatg tattacgttt ttcatggcta tccaaggaaa ggttacacgt
     1321 gatccgtcat cctccctcga tctgccagga tccggtctgc ggcatggatg tgatcattgc
     1381 tgagtctcgc tatccggcga tctatcatca aatgactttt cacttctgtt cggcacagtg
     1441 ccgggatcac tttctgaagg cgccggcctt atatgccgga gcagtccgcc gagaggacgt
     1501 caaggccatg cccaaacgcc gtgtgctgag gatcacgacg ggtgcagccc ttgacgagac
     1561 ctgccgccga ctccaggcaa tgatgggggt gagcgcgatt cgggccgagg cggatcggct
     1621 gcacatcgac tatgacctgc gccaggtgtc cctttcccag atcgaagccg aaatagaggc
     1681 ggccggcgtc atcctcaaag gtggcctaca cgcactgcgc cgtagcctgt ggcgcttcac
     1741 cgagcacaac gaactgcaga acgcggcccg caccgggccc caggcttgct gcaaccggcc
     1801 gccaacgccg cgctgagtgt gtccttcact ttaaggaggc cgtgatgatc gattcccgtg
     1861 aatcgtctca aactggaacg gatgcccacg tccatcaccg tcgcgggcgg tggatgtggt
     1921 ggctgtttgc cgcgatggcc ctgttctatc tgttggcgga acaccgtgcc catctgttcg
     1981 gcgttctgcc ctatctgctg gtgc
//
LOCUS       DAOQEW010000395         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_34920_length_1983_cov_8.609959, whole genome shotgun sequence.
ACCESSION   DAOQEW010000395 DAOQEW010000000
VERSION     DAOQEW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34920_length_1983_cov_8.609959"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..883)
                     /locus_tag="PLS35_20465"
     CDS             complement(287..883)
                     /locus_tag="PLS35_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNI21100.1"
                     /translation="MNGRNFWIGLVVGASAMGILGQWGPAVAGKDAIQPTCIPAAAVA
                     EYLHSVIQADRTFYTTDVVERMQMRGIAFAAENWRETSRLPLPAQYLLETGRLVAAGR
                     SGVQYRLISNWAINAKNRPMTDFERAGLTEILVNPDQPYTAVTTEGGAPVFQAIYADK
                     AASQSCVGCHNAHPNSPKKDFKPQDVMGGILLTIPLPQ"
     gene            1053..1610
                     /locus_tag="PLS35_20470"
     CDS             1053..1610
                     /locus_tag="PLS35_20470"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HNI21101.1"
                     /translation="MKKRMTPTLGRFVYLVACGLLSNPVLLWPTSGAQAESFNAKPGA
                     WEMTFTTQSSGMLVPPDVLAKMPPEQRAKVEQSMQARSGKPKTHSVKTCLTKEDLDQN
                     RIIKENDDDEPGCKTSVVSKSSAKLVLERTCPAPQASTSRMSVEAQTAELLVGSMDTD
                     RPRAGKVHIDFKSRWLGANCTGIAE"
     regulatory      1695..1797
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          452 a          599 c          547 g          385 t
ORIGIN      
        1 tcggtaatgg gacggggaag cgtggtgacc gttttcacct gcagcctttc ggttgagtcc
       61 ataccctgtg tcatgaagag acacggtctt aataattcta cgacaccctt tcgcggctac
      121 gcaatgaaat gagtcggagc agatggccta gaaggaactg gggcaattgg gcgaaggggg
      181 cgcgcgatgc gagaggaatg tgagaggtga tccatggact catccacatg tcggacttca
      241 acaagtctcg tggcaaccct tctaggtcct caacaggcac atccctctac tgcggcagcg
      301 gaatggtgag cagaatccct cccatgacat cctgcggctt gaaatctttc ttcggactat
      361 tgggatgggc attgtggcaa ccgacacagg actgggaggc cgctttgtcg gcataaatgg
      421 cttgaaaaac aggggcgccg ccctccgtgg tcactgccgt atagggctga tcaggattga
      481 cgaggatctc cgtcaggccc gctcgttcaa agtcggtcat cggccggttc ttggcattga
      541 tcgcccagtt gctgatcaac cggtattgca ccccgcttcg tcccgccgcc acgagccgcc
      601 ccgtttccaa caaatattgg gccggaagcg gaagccgcga cgtttcgcgc cagttttccg
      661 cggcaaaggc aatcccgcgc atctgcatcc gctcgacgac atccgtggtg tagaaggtac
      721 gatcggcctg aatgacgctg tgcagatatt cggccaccgc ggcggcgggg atacaggtgg
      781 gttgaatggc gtccttgccc gcgacggcag gcccccactg accaagaatt cccatcgcac
      841 tcgctcccac gaccagcccg atccaaaaat ttctcccgtt catgatgtgc ctccatttct
      901 gtgagcgcag tgcatcattg gtcttaagaa aagcttacgc ccgctcagga gagaaagcga
      961 gtgtgctgtc acacatcgtg atttctccga cagaactgcg tatgattgcg caggcaccat
     1021 cctagtcacc ccggagagga ggagaccgac ccatgaaaaa acggatgaca ccgacgcttg
     1081 gccgctttgt gtacctggtg gcatgtggac ttctgtcgaa ccccgtcctg ctctggccga
     1141 cctccggggc acaggccgaa tcattcaacg cgaaacccgg cgcctgggag atgactttca
     1201 ccactcaatc cagcggcatg ctcgtcccac cggatgttct ggcaaaaatg ccgcctgaac
     1261 aacgtgccaa agtcgaacaa tccatgcagg cacgttccgg caaaccgaag acccattccg
     1321 tcaaaacctg cctgaccaaa gaggacctgg accagaatcg catcatcaaa gaaaatgatg
     1381 atgatgagcc aggctgtaaa acctcggtgg tgtcgaaatc atccgcgaaa ctcgtccttg
     1441 aacgaacgtg tcctgctcct caggcctcaa cgtcccggat gagcgtggaa gcccagacgg
     1501 cagagttgct cgtgggaagc atggacaccg accgtccccg tgccggcaaa gttcacatcg
     1561 acttcaagag ccgctggctc ggagccaatt gcactggtat tgctgagtaa cgaccgtccg
     1621 catgagccat gaggcacctt gccactaggc cttccagaag attgacttcg ccctgacagg
     1681 tcggtacgat caccaacgac agctaggggt gccatccggc tgagagcccg agcgatcggg
     1741 cgaccctcac aacctgacct gggtaatacc agcgtaggga agcgaccggt tcactggctt
     1801 cgagatcgct tcaagccgca cccaagctgg atgcggcttt tttattttgg ccatcagacc
     1861 gctcaccttc gacgaaagga attccgccat gagcgaggcc catacacaga acggttccag
     1921 caacggacac ggcgccaaac cctcgacggc gcgcctgacc accaccccat tcgcggcctc
     1981 gcg
//
LOCUS       DAOQEW010000396         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_35866_length_1955_cov_9.799474, whole genome shotgun sequence.
ACCESSION   DAOQEW010000396 DAOQEW010000000
VERSION     DAOQEW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35866_length_1955_cov_9.799474"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1406)
                     /locus_tag="PLS35_20475"
     CDS             complement(312..1406)
                     /locus_tag="PLS35_20475"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNI21102.1"
                     /translation="MYEDQGGAVELWQYGGFADVSYASTGYHPENHQWRSKETSTRTN
                     ELAPNIGLLYVRKDPAEQSRWGVELAFQAGYDTDDLVPEPKADGTRPVSGADTLRHVA
                     RANVSYLAPIGRGVTFSAGLMKGSKTYEEFYAKYNLNYTRAYITDYNPNFVMGLGASY
                     PITKSLELGVYVINAYRHLAHTNDAPSYLSKMEWRVSDHLTVYENLYYGPDQRATSVD
                     YWRTFSDSTVEWRKPDWRVALSYNVGTEKMADVSGSPRAVWMGAALFTQRHLVGPWSI
                     AVRPECYWDPQGRMTEHEQLLWANTTTLEYKKHLGQQLIIVRLEHRYDRSTGSQGGFF
                     RDGFSAPGVPRLAADQHLTILGVILAFDSA"
     gene            complement(1556..1783)
                     /locus_tag="PLS35_20480"
     CDS             complement(1556..1783)
                     /locus_tag="PLS35_20480"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNI21103.1"
                     /translation="MGLDYAADDELASRIDQALLATDELNLSRIEVSVEPGVIYLSGI
                     SDDHDSKVQAEQAAQAYAEGRKIVNKIEVDF"
BASE COUNT          439 a          551 c          551 g          414 t
ORIGIN      
        1 cccaggagag accggcaacc accacccagg tcagcaggag gatcaatact tttggaagac
       61 gagaggctgg acaacgcaag acgatcatac tcatgttaac gattggcaag ttgcgtagtc
      121 cccttctcgg acccgcaatg actggagagt accataggcc gcgcctagca acaagcaacg
      181 cgacatgtcc ttgcgcctcg cttgttcacg catatgacga tgagcgaata ggtgctgtag
      241 gcggcggaca gggtgagcaa tccctcgatg tgcctggcat ttgtcgaagt ctgcccccgt
      301 aaaacgggct gctaggcgga gtcgaaggcg agtatcaccc ccaggatcgt gagatgttgg
      361 tctgccgcca gccgcggcac acccggcgca gagaacccgt cgcggaagaa tccgccttgc
      421 gacccggtcg atcgatcata ccggtgttcc aaacggacga tgatgagctg ttgtcccagg
      481 tgtttcttgt attcgagggt ggtggtatta gcccatagca attgctcatg ctccgtcatc
      541 cggccttggg gatcccagta acactcgggc cggacagcga tgctccaagg gccaaccagg
      601 tgtcgttgcg tgaataaggc ggcgcccatc cacacggctc gtggagagcc ggagacatcg
      661 gccatctttt ctgttcccac gttatacgac aaggccacgc gccagtccgg tttgcgccac
      721 tccacggtgg aatcggagaa ggtccgccaa tagtccacgg aggtcgcccg ctggtccggg
      781 ccgtagtaga ggttttcata gaccgtgaga tggtcagata cgcgccactc catcttggaa
      841 aggtaactgg gagcatcatt ggtatgcgca aggtgccggt acgcattgat cacatagacg
      901 ccgagttcca gtgacttggt gatgggatag gaggccccca atcccatcac aaaattaggg
      961 ttgtaatcgg taatgtaagc ccgcgtgtag ttgaggttgt atttggcata aaactcttca
     1021 taggttttgg aacctttcat cagaccggcc gagaaggtga caccgcgccc gatcggcgcc
     1081 aggtaactga cattcgcccg agccacatga cgcaaggtat ccgcacctga cacaggtcgt
     1141 gtcccgtcgg cttttggctc agggacaaga tcatcggtgt catatccggc ttggaaggcc
     1201 aactcaactc cccagcggga ctgctctgca ggatcttttc gcacatacag cagtccgata
     1261 ttcggagcca gctcatttgt cctcgtggat gtttccttgc tgcgccactg atgattttcg
     1321 ggatgatacc ccgtagaggc gtagctgaca tcggcaaacc cgccatactg ccagagctca
     1381 accgcgccgc cttggtcttc gtacatgata caaagaaagc acgcgacgag aaccgccagg
     1441 cacctatgcg ctcgcggggc agggcaggct ttgtcacggt acccgtgctg gaaacgatga
     1501 gtcctcgtcg ccagtaaccg catgtcatcc cgagatgacg aggcaatgtg aggcatcaaa
     1561 aatcgacctc aattttattg acgatttttc tgccttccgc ataggcctgc gccgcctgtt
     1621 ctgcctgaac cttactgtcg tgatcgtcgg atatgccgct gagataaatc acccccggtt
     1681 cgacggagac ttcgatgcgg ctgagattca gttcatctgt ggccagtagc gcctgatcga
     1741 ttcgtgaggc cagctcgtca tcggcggcat aatcaagccc cacgcagcca gctagtccaa
     1801 tgaaagcaat gaggcccagt ggaagcaatg ccagggaacc agcatgaggc gacgatcgcg
     1861 gcctaggaga cagcttcttt agcacgcact actccgccgc tttgggaatg atcgagattc
     1921 caccattggt ttcaagcacg gcatatttga tctgt
//
LOCUS       DAOQEW010000397         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_36011_length_1951_cov_5.200422, whole genome shotgun sequence.
ACCESSION   DAOQEW010000397 DAOQEW010000000
VERSION     DAOQEW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36011_length_1951_cov_5.200422"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1951)
                     /locus_tag="PLS35_20485"
     CDS             complement(<1..>1951)
                     /locus_tag="PLS35_20485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNI21104.1"
                     /translation="SLVDAQHRRLVELLNGLASHVAFHNDDYRLDALFDDLTDYARYH
                     FQTEESIWESLLGGEAAESAHRGAHADFVAEVARLRKSRTGGDDFAVAENALGFLARW
                     LASHILQADRFLAYAVEAVSQGLSRSASLERARERMDGSTHALIEVILSIYSTLSTNT
                     LRLMRELAARQKADEARMRSEERLKIALNAARQAWFEADIPTGAIALDPRYEEMLGYK
                     PGELHITLSNWLDGIHPEERTAVYAEFKALVEKDGPIAIEYRRQAKTGEWLWFQSTVV
                     VSERDHDGRPTKISGINMDVTDRKKIGMEAAKTHTLLSEAIDSLAIGFTIYDEEDRLL
                     LCNEAYRRYYAASQDLIVPGNTFAEIVRRGAQRGQYEDAVGREEEWVQERVRIHRQAD
                     GTLIEQHLTDGRWLLIVEYRTPSGFIVGNRVDITDRKNAEIALREQARRLNEAQRLAL
                     VGDWHFDLTSQRLTWSPELFRIFERDSDGEQLSWDAVLDAFHPEDRAEFTRAYRRSLV
                     SRLPSSVTARLQLPEERIKFVLVRWENRYDPRGLPESSSGTVQDVTERRLNEDALRKA
                     SRYARSLLEASVDPLVTISPEGKINDVNQATERVTGLGRSGLIGSDFSDYFTQPELAR
                     AGYRKAFSEGSVRDFPLAIRHASGHV"
BASE COUNT          353 a          608 c          591 g          399 t
ORIGIN      
        1 cacgtgcccg gatgcgtgtc ggatggccag cgggaagtcc ctgacgctgc cttccgaaaa
       61 cgccttcctg tagccggccc gggcgagttc cggttgggtg aaataatcgg agaaatcgct
      121 gcctatcagc ccgctgcgac ccaagccggt gactcgctcc gtcgcctgat tcacgtcgtt
      181 gatcttgccc tccggactga tggtcaccaa cgggtcgacg ctcgcttcaa gcaggctccg
      241 cgcgtaccgg gaggctttgc gcagggcatc ttcattgagg cggcgctccg tcacatcctg
      301 cacggttcct gacgaactct cgggcaatcc ccgtgggtcg taccggttct cccatcgaac
      361 caatacgaat ttgatccgtt cctcaggcag ttggagccgg gcggtgaccg aggagggcaa
      421 acgcgacacc agggagcgtc ggtaggcacg cgtgaattcc gcccggtcct ccgggtgaaa
      481 tgcgtccaag acggcatccc aggaaagctg ttcgccgtcc gaatcccgct cgaagatgcg
      541 aaacagttcg ggtgaccaag tcagtcgttg gctggtcaaa tcgaaatgcc aatccccgac
      601 gagcgcgagc cgttgtgcct cgttgagccg acgggcctgc tcgcgcaacg caatctcggc
      661 gttcttgcgg tccgtgatgt cgacccggtt accgacgatg aatccgctgg gtgtcctgta
      721 ctcgacgatc agcaaccagc gtccgtccgt caggtgttgc tcgatcaaag ttccgtctgc
      781 ctgccggtgg atgcgcacgc gctcctgcac ccactcctcc tcgcggccta cggcatcttc
      841 gtactgcccg cgctgcgccc cgcggcgcac gatttccgca aacgtgttgc cgggaacgat
      901 gaggtcctgg cttgcggcat agtatcggcg ataagcctcg ttgcaaagga gaaggcggtc
      961 ctcctcgtcg taaatggtga aaccgatggc gaggctatcg atggcctcgg acaacaaggt
     1021 atgtgtcttc gcggcctcca taccgatctt cttccggtcc gtcacgtcca tgttgatgcc
     1081 cgaaatcttc gtcgggcggc cgtcatggtc cctttcggac acgacgactg tggattggaa
     1141 ccagagccac tcgccggtct ttgcctggcg gcggtactcg atcgcgatgg gaccgtcttt
     1201 ttccaccagc gccttgaact cggcatatac ggcggtgcgc tcctccggat ggatgccatc
     1261 gagccaattt gagagggtga tatggagttc cccgggtttg tagccgagca tctcctcgta
     1321 ccgggggtcc aacgcaatcg ctccggtggg aatatcggcc tcgaaccacg cttgtctggc
     1381 tgcgttcaac gcgattttca ggcgttcctc gctgcgcatc ctcgcttcgt ccgccttttg
     1441 ccgtgccgcc aattcgcgca tcaacctcaa tgtgttggtg gagagcgtgg aatagatgga
     1501 cagaattact tcgatgagcg cgtgcgtgct gccatccatg cgttccctgg cgcgctccag
     1561 agacgcactg cgcgacaagc cctgcgatac ggcctcgacc gcataggcaa ggaaccggtc
     1621 tgcttgcagg atgtgggacg cgagccaacg cgccaggaaa ccgagcgcgt tctcggcgac
     1681 ggcaaagtca tctccacccg tgcgggattt ccgcaggcgt gcgacctcgg cgacaaaatc
     1741 ggcatgtgcg ccacgatggg cagattcggc cgcctctcca ccgagcaggg attcccagat
     1801 ggattcctcc gtctgaaaat gatagcgggc gtaatcggtc agatcatcga acagcgcgtc
     1861 gaggcggtaa tcgtcgttgt ggaaggcgac gtgactcgcc aagccgttga gcagttccac
     1921 caatcggcgg tgctgggcgt cgaccagaga c
//
LOCUS       DAOQEW010000398         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_36081_length_1949_cov_7.574446, whole genome shotgun sequence.
ACCESSION   DAOQEW010000398 DAOQEW010000000
VERSION     DAOQEW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36081_length_1949_cov_7.574446"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLS35_20490"
     CDS             <1..260
                     /locus_tag="PLS35_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21105.1"
                     /translation="QRTPDEMTEVPASRFVWDLGNVGEEGAFISLVFILRDEPAFSAS
                     VKPSQCNLPMLWTDENIVAVGVASPLRLLAKTAGSSWSEAC"
     gene            complement(431..622)
                     /locus_tag="PLS35_20495"
     CDS             complement(431..622)
                     /locus_tag="PLS35_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21106.1"
                     /translation="MAFIEQHGRKIYQFSDNPALPRHQYQILSGIPLPDIPRYQETPL
                     EYHARTGYPLDLCRRYLNP"
     gene            complement(1758..>1949)
                     /locus_tag="PLS35_20500"
     CDS             complement(1758..>1949)
                     /locus_tag="PLS35_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21107.1"
                     /translation="AIYSEGRANQRQDLPEAYYWAQLAARHQFRPAKELADTLSPQLS
                     HDERARIDARIKKWKRVVS"
BASE COUNT          409 a          461 c          640 g          439 t
ORIGIN      
        1 gtcagcgcac tccagatgaa atgacagaag tcccagcgtc tcgcttcgtc tgggacctgg
       61 gcaatgtggg cgaggaggga gcgttcatct ctctcgtttt tatcctccgc gatgagccag
      121 ccttctccgc ttccgttaaa ccatcgcagt gcaatctgcc gatgctctgg acggatgaaa
      181 atattgtagc ggtaggagtc gccagtccac ttcgtctgct tgctaaaact gcagggtctt
      241 cctggtccga agcgtgctga gcacacgtgt gtccattcgg cgggattaac ctggatcgca
      301 acataccggt ttccgtaagg cggatggagg agatatccag attcttgaat cctcatcggt
      361 ggaatgggag gttggttcat acgtcctcat gtgcagtagt ggcaggaatg ccctgttgct
      421 acttgggttg ttaggggttg agatagcgtc ggcagagatc gagggggtat ccggtacgcg
      481 cgtggtactc cagcggcgtt tcttggtacc gtggaatgtc gggaagtgga atgcccgaga
      541 ggatctggta ttggtggcgc gggagtgcgg gattgtcgct gaactgatag atctttcttc
      601 cgtgctgttc aatgaaagcc acgcaagtgt ctctgctgaa actccgaggg agccgatacc
      661 gttcgagaat gctcgcccaa agtaagccgt gcgcatagag atcaaagcag agtgtataga
      721 gaaaatgcgg gtccccaatt ggcaggaggg ggacgtacaa cgatccgtct ggttgaggtg
      781 tcacttctcg gacatgggca tagacaattg gcggttccgg tttttgtatc gactctgttg
      841 cggggagaga ctgggctttc cggatgtggg cgcactcggc tcgtcggtag gtccaatgtg
      901 gacaggtgca ttgccagtca ttggcctggc ttcgagtgac ggtatagcaa agccctggct
      961 tggaattcga cgcgatggag aaggattcgg cgtagggcat acagccttcc tgtgcgactt
     1021 gagcctgact agaggggagt gaaccagact accaggtcgg caggatctgt ggagcggccg
     1081 tcgtctgagg catgagatgg gttggtatcg tgatggggca gcctgacaga cgtgtgatct
     1141 accgggatgc agtgaaatca aaaaaggaag cggcggccgg ctcagtcgga gcggggttca
     1201 tccaccaact tgggtcatcc agtgatggtg catacgagac tatggggtcg atacatcgac
     1261 cacggtcttt aatttggtcg aggatgtcct cctcactcgc aagatactcg ctatcggtga
     1321 aggtctcaca tcgaaggtga ggcaccggtg gggcgatccc aggaggaagc ggggcgcctg
     1381 ggagataccc gagaaggtag aggaggacaa cggtcgacat ggtcggcata gcgcctcatc
     1441 aactcgatgg acaccattct atcgaagcgt gtgaacgggg gcaatcggcc ctgcgggctt
     1501 agggatgggg gtcgtctttc aatggagagg ctgacaaact cacgtcggtg tgaggccgtt
     1561 cactctcttg gacgaggatc tgcaacatgg cgagatcgac acgttcttgt tgcgcacggg
     1621 cttcctcgtg ggcgagacga ggagcggatg cgcctcccgg caggtatcga atgatggtcg
     1681 agataatctg acgaaggcta gtggctaggg gtcccatacc aacacagtaa atccgaatcg
     1741 gttcggcaca ggataggcta cgagacgacg cgcttccatt tcttgatgcg ggcatcgatg
     1801 cgcgcgcgct catcatgtga gagctgtgga ctgagcgtat ctgcgagttc cttggccggt
     1861 cggaattgat gccgggccgc aagctgcgcc cagtagtagg cttcaggcaa gtcttgccgt
     1921 tggttggccc tgccttccga ataaatggc
//
LOCUS       DAOQEW010000399         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_36155_length_1947_cov_7.924947, whole genome shotgun sequence.
ACCESSION   DAOQEW010000399 DAOQEW010000000
VERSION     DAOQEW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36155_length_1947_cov_7.924947"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLS35_20505"
     CDS             <1..360
                     /locus_tag="PLS35_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNI21108.1"
                     /translation="QVMMATTNGTFGSGTFGISSGTSGCTNDGQVMADQKTTMFALLN
                     FENLTQEMAQGKGEHLASLATLMGVPAEQHAAFFALTQERYTALVQDGETSSVALVKT
                     LNDAVSKSPVLAQAVSR"
     gene            500..>1947
                     /locus_tag="PLS35_20510"
     CDS             500..>1947
                     /locus_tag="PLS35_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNI21109.1"
                     /translation="MSQLLQRAHQAKLADEREWHLLLHYRANLFGGFTSEQDEPGFFL
                     SPDGKTDPEAELDSTLTQFFSPDLVGRSKQPAQCAFVARYAWLHERLAFDPGQLPVMR
                     CDRFERWFEEFHARSVTLVFPSAFMNNPASMFGHTLLRIDQEGQTPHTRILAYTINFA
                     ADVPKDEGLAYPVRGIFGGYRGYFSTIPYYLKVQEYRDMENRDIWEYRLNFDAPQIRR
                     LLMHAWELGNASFDYFFFKENCSYHLLSLLEYADPSLRLTDKFRFWTIPADTVRLLAD
                     QPGLVTDTAFRPSRVTLIRRKREQLTVAEHGLVQRLVRDAAVAQSDDVRSLPLSRQAF
                     VLDVASDYVRYTGERDESQATAARDHNRQLLTARSLLRIPSEELSILPFATQPELGHK
                     TSRASLGGGWRNNDSFEEVSVRTAYHDLLDPEPGYTPDAQIEIGSISLRHYNRADQAR
                     IERATLLNVVSLSPIDSLFRAPSWKLNVGMQT"
BASE COUNT          385 a          578 c          541 g          443 t
ORIGIN      
        1 caggtgatga tggccacgac gaacggcacg tttggcagcg ggacattcgg gatcagctcc
       61 ggaacgtccg gttgtacgaa tgatggtcag gtcatggcgg atcagaagac caccatgttt
      121 gccttgttga acttcgaaaa cctgacgcag gaaatggcgc aaggaaaagg cgagcacttg
      181 gcgtccctcg cgacgctgat gggcgtgcct gccgaacagc atgcggcctt cttcgcctta
      241 acccaggagc gctacacggc actggtgcag gatggtgaaa cctcgtccgt ggccttggtg
      301 aagacgctga atgatgcggt ctccaagagc ccggttctcg cccaagccgt cagccgctaa
      361 ttgattcatc gcagggcttc ggtcattggc cggggccctg cgctgcattc tgtctccaat
      421 cgtgcatatt ttgttgatcc tcctagtcgg ccttttcctc gcgacttcca ccgaggcgca
      481 agagccgccg gcgccgtact tgtcccagct ccttcaacgc gctcatcagg ccaagctggc
      541 agatgagcgc gaatggcatc tcttgctcca ttatcgagcc aatctcttcg gtgggtttac
      601 cagtgaacag gacgagccgg gtttctttct gtctcctgac ggcaagacag atcccgaggc
      661 tgaacttgat tcaacgctga cacaattttt ctcgccggac ctggttggcc gctccaaaca
      721 gccggcgcaa tgtgcctttg tcgcgcggta tgcgtggctc cacgagcgtc ttgcctttga
      781 cccgggacaa cttccggtca tgcgctgcga tcggttcgag cgatggttcg aggagttcca
      841 tgcccgatcc gtcacgctgg tatttccctc ggcctttatg aataacccgg cctccatgtt
      901 tggccatacg ctgttgcgca tcgaccagga aggtcagacg ccgcatacgc gtatcttggc
      961 ctataccatc aattttgcgg cggatgtccc caaagacgaa ggattggcgt atccggtacg
     1021 cggcattttc ggaggctatc gcggatactt ttctacgata ccctactacc tgaaagtgca
     1081 agagtaccgg gacatggaaa atcgcgatat ctgggaatac cgtctcaact tcgacgcgcc
     1141 ccaaatccgg cgattactca tgcatgcctg ggagctcggg aatgcgtcat tcgactattt
     1201 tttctttaag gaaaactgtt cttaccacct cttgtcgtta ctggaatatg ccgacccttc
     1261 gttacgcttg accgacaagt ttcgcttctg gaccattcct gcagacaccg ttcgcctgct
     1321 ggccgatcaa ccgggactgg taacggacac cgcgtttcgc ccctcgcgtg tgacgctcat
     1381 tcgccgcaag cgcgaacagc tcaccgtcgc tgagcatgga ttggtccagc gattagtgcg
     1441 tgatgcggca gtggcgcagt ccgatgacgt ccggagcttg ccgttatcgc gccaggcgtt
     1501 cgttctggat gtggcatccg actatgtgcg ctatacgggt gaacgagatg agtcgcaagc
     1561 gacagcagcc cgcgatcaca accgtcaact tctgaccgcg cgcagcctct tgcgaattcc
     1621 ctccgaggag ctctcgattc tgccgtttgc cacgcaaccc gaattagggc acaagacttc
     1681 ccgcgcgagt ctcgggggcg gctggcgtaa taacgatagc tttgaggagg ttagcgtgcg
     1741 gaccgcgtat cacgatttgc tcgatcccga gccgggatat acgccggatg ctcaaatcga
     1801 gatcggctca atcagcctcc gtcactataa ccgggccgac caggcccgga ttgagcgggc
     1861 aaccctgctg aacgtggtat ccctctcgcc catcgacagc ctgtttcgtg ccccatcctg
     1921 gaaattgaat gtcgggatgc agaccat
//
LOCUS       DAOQEW010000400         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_36839_length_1929_cov_10.453575, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000400 DAOQEW010000000
VERSION     DAOQEW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36839_length_1929_cov_10.453575"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLS35_20515"
     CDS             complement(<1..290)
                     /locus_tag="PLS35_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21110.1"
                     /translation="MQPKLKSKVRCTDGEVGEVRRVIMDPLSHEISHIVVGGGTAGVV
                     ERQVPMGQVQAVSDEVVTLRGTSAEFAALPAFKRDEYVTTHEVEIAHLEDRI"
     gene            complement(296..1102)
                     /locus_tag="PLS35_20520"
     CDS             complement(296..1102)
                     /locus_tag="PLS35_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNI21111.1"
                     /translation="MGNIIKKLVVGVVVGGILFGATNALGFPFVFQALFFGYAMLGAI
                     VFMILDFPTLKPFGGVKAVGALLVFYAILSVVYIAGASMWPQYDPEDEKGKIEKILKP
                     KREHYEAGKVEVLMQRAKALDEKAKELTARLQALGAAQAPADQKAGGESAPTATTAAA
                     SGDIAKLGEEQWQLQECYNCHKLNGEGGKKRGPELDNIGNLLPAAEIARKILDPKSYK
                     AEGFEQEYEKGKMPDKYKDLMEPKDVDALATWLAGYKNASVNTPKPIKMK"
     gene            complement(1144..>1929)
                     /locus_tag="PLS35_20525"
     CDS             complement(1144..>1929)
                     /locus_tag="PLS35_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21112.1"
                     /translation="AMIGLIFLASNYYIWLSMKRIEGVERVRMTVVAPIVMVLLPIVM
                     TKVLTDYPVPDATSLAFLLPLLLAPVVLGRFIPLTVSSSTVIKIGFLMVVVGNAIWMT
                     PHGFVPTGAKLVAELELPSDWNFLALMPAKNSAAFTLVFVTVVNYVIYNRAVSQGTIV
                     WGKIDFASQFVLVFLAFSAIWTMGLMGAVRSLLRKYFHTYNLLPDFTAESFTPTLSYS
                     AWWITGITIVFYAVVSFAIIVTLRPSDSKGHAHEGSPVPAGAK"
BASE COUNT          428 a          591 c          500 g          410 t
ORIGIN      
        1 tggatccggt cttcaaggtg ggcaatttcc acctcgtgcg tcgtcacgta ctcatcgcgc
       61 ttgaaagccg gcaaggcggc aaattccgcc gatgtgccgc gaagcgtgac gacttcatca
      121 ctaaccgctt gtacctggcc catcggaacc tggcgctcga cgacgcctgc cgtccccccg
      181 cccacgacga tatgactgat ttcgtgggag agcgggtcca tgatgaccct gcgcacctct
      241 ccgacttcac cgtccgtaca tcgaactttt gatttgagtt tcggctgcat gatgctcact
      301 tcatcttgat cggcttcggg gtattcaccg aagcgttctt gtatccggcc aaccaggtcg
      361 ccaatgcatc cacgtccttc ggttccatca ggtccttata cttgtcgggc atcttgccct
      421 tctcgtattc ctgctcgaac ccttccgcct tatagctctt cggatcgaga atctttctgg
      481 cgatttctgc cgccggcaga aggttgccga tgttgtccag ctccggaccg cgtttctttc
      541 caccttcgcc attcaacttg tggcagttgt aacattcctg cagctgccat tgctcttcac
      601 ccaacttggc gatatcacca ctggcggctg ccgtggtggc ggtcggcgcg ctttcgccgc
      661 cggccttttg gtcggcaggc gcttgagctg cccctaatgc ctgcagacga gcggtcagct
      721 ccttggcctt ttcatccaga gccttggccc gctgcatcaa cacttcgacc ttaccggcct
      781 cgtagtgctc gcgcttcggc ttgagaattt tttcaatttt gcccttttca tcttccgggt
      841 catattgcgg ccacatggag gcgcccgcaa tgtagaccac ggagagaatg gcataaaaca
      901 cgagcaatgc cccgactgcc ttgactccgc cgaatggctt gagcgtcgga aaatcgagga
      961 tcataaagac aatggcgcca agcatggcgt agccgaaaaa cagcgcctga aagacgaacg
     1021 gaaagcccag cgcgttggtg gcgccgaaca gaattccgcc gacaacgact cctacgacca
     1081 actttttaat aatgtttccc atgaatgccc tcgtttaccg cccttcccag tatggtgacc
     1141 gccctacttg gcccctgccg gaaccgggct cccctcgtgc gcgtgaccct ttgaatctga
     1201 cgggcgcaag gtcacgataa tcgcgaagct gaccactgca taaaagacga tcgtaatacc
     1261 ggtgatccac cacgcggaat aagacagcgt tggcgtaaag gattccgccg tgaaatccgg
     1321 caacaggttg tacgtatgga agtacttccg caacagggag cgcacggcgc ccatcaggcc
     1381 catggtccaa atcgcgctga atgcgaggaa gaccaggacg aattgcgaag cgaagtcgat
     1441 cttgccccac acgatggttc cctgagaaac cgcccggtta taaatgacat agttcacaac
     1501 ggtgacgaag accaacgtga acgccgcgga atttttcgcc ggcatgagcg ccaggaagtt
     1561 ccagtcagac ggcagctcca attccgccac cagcttggcg ccggtgggta cgaatccgtg
     1621 gggcgtcatc cagatagcgt taccgacaac gaccatcaag aaaccgatct taatgacagt
     1681 gctcgacgaa actgtgagcg ggataaatcg ccccagaaca accggcgcca gcagaagcgg
     1741 cagcaggaag gccagcgagg tggcatccgg cacgggatag tcagtcagga ccttcgtcat
     1801 gacaatgggc agtaacacca taacaatcgg cgccaccaca gtcatacgaa cccgctccac
     1861 accctcgatc cgcttcatac tcaaccagat gtagtaatta ctggcgagga agatgagacc
     1921 gatcatggc
//
LOCUS       DAOQEW010000401         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_37047_length_1923_cov_8.880621, whole genome shotgun sequence.
ACCESSION   DAOQEW010000401 DAOQEW010000000
VERSION     DAOQEW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37047_length_1923_cov_8.880621"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="PLS35_20530"
     CDS             <1..1176
                     /locus_tag="PLS35_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HNI21113.1"
                     /translation="GIGAMLVGGVWTLIQVRGPILHSLRQLLSLYAARRDDRGASAPL
                     RTERDAGVVWLIGLTVASLIPMVFLYRHLLDHSLFAGAGLTVLMVVTAFLFSAVAGYM
                     AGLVGSSSNPVSGVTIATIMLAALLLLAILGREHPAGPAAALLVGAVVCCAAAMGGDN
                     LQDLKTGHLVGATPWKQQVMQVIGVATGAVVIVPVLSLLQAKYGIGVATAAHPHPLSA
                     PQATLMANLAKGVFGGSLPWHLVGIGMALGVVIIALDQRQAQRQAQLRFPVLAVALGM
                     YLPLKLSAAIMFGGLLAEVVRRGRPASETPTDDQGLLCAAGLVTGEAMVGILLAVPIA
                     LSSVWPSLAGDPFQLFADPPLGGWPGLLALALVGLFLVRAAQAAGGAGNADATPRGV"
     gene            complement(1203..1673)
                     /locus_tag="PLS35_20535"
     CDS             complement(1203..1673)
                     /locus_tag="PLS35_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNI21114.1"
                     /translation="MPHIGALVRAWRVSQKCSEQAFAQRAGITPNLLESLESEQLDPT
                     ASTLEAFAKALQIPLSWLFIHPTEFDLLCKDDDEEPAPLSTLTGADPVLDRLLLAAGH
                     DRTLFVLLTALMQSGDPKLLRAAEVSLRSLVKQSKQATVPWQSRPPGHFEPPSD"
BASE COUNT          325 a          522 c          643 g          433 t
ORIGIN      
        1 gggatcggcg ccatgctcgt cggcggagtc tggacgctga ttcaagttcg agggcccatc
       61 ctgcacagtc tccggcagtt gctttcgctc tatgctgctc gtcgcgacga tcgtggtgcg
      121 tcggcgccac tccgcaccga gcgggatgcg ggcgtggtct ggctcatcgg tctgacggtg
      181 gcttcgctga tccctatggt attcctgtac cgccacctgc tggatcacag cctattcgct
      241 ggcgcgggat taaccgtgct gatggtggtg acggcgtttc tcttttctgc cgtggccggg
      301 tatatggcag gcctggtcgg cagttcgagc aatccggtgt cgggtgtcac gatcgccacg
      361 atcatgctcg cggcgctgct tctattggcc atcctggggc gtgaacatcc ggccggtccg
      421 gcggccgcct tgctggtggg ggcggtcgtg tgttgcgccg cagccatggg tggcgacaat
      481 ctgcaggatc taaaaaccgg acacctggtt ggtgcgacgc cgtggaagca acaggtgatg
      541 caggtcattg gagtggcgac cggtgcggtg gtgatcgtac ccgtgctgtc cttgctacag
      601 gcgaaatatg gaatcggagt ggcgaccgcc gcgcatcccc atcccctgag tgccccgcag
      661 gcgactttga tggccaatct tgccaaggga gtgttcggcg ggtcattgcc ctggcatttg
      721 gtcggcatcg ggatggcgct cggcgtggtg atcatcgcct tggatcagcg gcaggcccaa
      781 cgtcaggctc agcttcgttt cccggtgttg gctgtcgcgc tgggtatgta cctgcctctc
      841 aaattgtcgg cggcgatcat gttcggcggg ttgctggccg aggtagtgcg aaggggcaga
      901 ccggcttccg agactccgac tgacgatcaa gggctcctat gtgctgccgg gctcgtcacc
      961 ggggaagcga tggttggtat cctgctggca gttcccattg cgttgagctc tgtgtggccg
     1021 tcgttggcag gagatccgtt tcagttgttc gccgatccgc cgttgggcgg ctggccgggg
     1081 cttctcgccc tggcattggt cggcctcttc ttagtgcgag ccgcacaagc cgcaggaggt
     1141 gcaggcaatg ctgacgccac ccctaggggc gtgtgacgtc gcgtgaatga ggaggacgga
     1201 agctagtcgc tgggtggctc aaaatgtccc ggcggccggg attgccatgg gacggtggcc
     1261 tgtttggatt gtttcacgag gctccgcagg ctgacttcgg cggcacggag aagtttcggg
     1321 tcgccgctct gcatcaatgc ggtgagcagc acgaacagcg ttcgatcatg cccggctgcc
     1381 aggagcaacc tgtccagcac ggggtctgcc ccggttagcg tggagagcgg agccggttcc
     1441 tcatcgtcgt ctttgcagag gaggtcgaat tccgtcgggt gaatgaacag ccaggaaagg
     1501 ggaatttgca gagctttggc gaaggcttcc agcgttgacg cagtgggatc gagctgttca
     1561 gactccaatg attctaagag attcggggtg attccagctc gctgggcaaa ggcctgctcc
     1621 gaacattttt gagacactcg ccatgcgcga accaaggctc cgatgtgtgg catggcgccg
     1681 atcatactgc atcccttttc ggcactgcaa cgtcgctttt tctcatgttg agggatactt
     1741 gtctgcaaga tcaatacgtt atgatccatt ctcctaggta gaacaaaggc atacaaatcg
     1801 ggtagaatga gccgttctta ttgatatccc cagcgaggag gatcagccaa tgttaggaac
     1861 cgacattcgc ggcattatgg ccgaagagga agaagtccag cggcggcagg aagccttgca
     1921 gtc
//
LOCUS       DAOQEW010000402         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_38301_length_1889_cov_6.893675, whole genome shotgun sequence.
ACCESSION   DAOQEW010000402 DAOQEW010000000
VERSION     DAOQEW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38301_length_1889_cov_6.893675"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1177
                     /locus_tag="PLS35_20540"
     CDS             86..1177
                     /locus_tag="PLS35_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNI21115.1"
                     /translation="MRGEVDPQSSMFHYFSVESRIPADHPLRRVKKLAESALSAISAE
                     LDGLYARTGRPSIAPERLLKAQLLIALYSVRSDRLFCEQLDYNLLFRWFLDLDLESGG
                     LDQSNFSRLRERLVETDIARRFFDEVVRLARKDKLLSSDHFTVDGTLIDAWASFKSFK
                     RKDDIDPPKPGDGTGMVDFKGEKRSNATHQSTTDPDAKLMRKGNGQPAKLSYGGHALM
                     ENRSGLCVDLLITDATLAEHQAARQLLTRARRRRIHPKTLGADKGYHVKDFVQHLRDH
                     NIRPHIARIEGRKTPGLDGRTTCTEGYKVSQRKRKRVEEIFGWLKTVGGMRKTRFIGQ
                     AKTQIAAYLSAAAYNLLRIAKLQSAGSVA"
     gene            1444..>1889
                     /locus_tag="PLS35_20545"
     CDS             1444..>1889
                     /locus_tag="PLS35_20545"
                     /inference="COORDINATES: protein motif:HMM:NF019902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21116.1"
                     /translation="MSIFTSFQTRYETTREEEMSVQEYLELCKSDKMAYASSAERMLQ
                     AIGEPELVDTRLDPRLSRIFSNKMIKIYPAFRDFYGMEESIENIVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSLAEKLKSLVEKIPFYAIKGSPVHESPLGLF"
BASE COUNT          402 a          579 c          556 g          352 t
ORIGIN      
        1 gctagcaggt tgatgcaaaa cccagtgaac gatccgacga tccacctgtc ctagcagtac
       61 ccctcaaacc ccaggcacag taccgatgcg cggcgaagtc gatcctcagt cctcgatgtt
      121 ccactacttc tcggtcgaat cccggattcc ggccgatcac ccgttgcgcc gggtgaagaa
      181 actggccgaa tcggcgctgt cggccatttc tgcggaactc gacggccttt acgcccggac
      241 gggtcgccct tcgattgccc cggagcgcct gctcaaggcc caattgctca tcgcccttta
      301 ctcggtgcgc tccgaccgtc tgttctgtga gcagctcgat tacaacctgc tgttccgctg
      361 gtttctcgat cttgacctgg agtcgggcgg gctcgatcaa tccaatttca gccgactgcg
      421 cgagcgcctg gtggagaccg atattgcccg acgcttcttc gacgaagtcg tgcgtctggc
      481 ccgcaaggac aagctgctct cgtccgatca cttcacggtg gatggcacgc tgatcgacgc
      541 ctgggcgtcc ttcaagagct tcaagcgcaa ggacgatatt gatccgccca agccggggga
      601 cggcaccggg atggtggatt tcaagggcga gaaacgttcc aacgccaccc accagagcac
      661 caccgatcct gacgcgaagc tcatgagaaa aggcaacggt cagccggcca agctcagcta
      721 cggcggccat gccctcatgg agaaccggag cggcctgtgc gtggatctgc tcatcaccga
      781 cgccacgctg gccgaacatc aggccgcccg ccagttgctc acccgcgccc ggcgccgtcg
      841 gattcatccc aagaccctgg gggccgacaa gggctaccac gtgaaggact tcgtccagca
      901 cctgcgggat cacaacatcc ggcctcacat tgcccggatc gaagggcgca agacaccggg
      961 cctggatgga cgaacgacct gcaccgaggg ctataaggtc agccagcgca aacgaaagcg
     1021 cgttgaggag atcttcggct ggctgaagac ggtgggcgga atgagaaaga cacgcttcat
     1081 cggccaggcg aaaacccaga tcgccgctta cctgtcggca gcggcgtaca acctgctgcg
     1141 gatcgccaaa ttgcagtccg cggggagcgt ggcatgatgc ggcacccgga atcgggtgaa
     1201 aacccattcc ctcgcccatt cgatgggcat caaaacggga ttgggaatcc aattccggga
     1261 atgaagtcat gagaagtcgg atggcgaggg ccactgctgg tgcggtttct cggggttttg
     1321 catcggcctg ctagctgacg aggggctcag ctcaacgttc tgcgtcatgt gtcgttatcc
     1381 agtctgttgg acaacgaaga aaccggcacc cgtgcctggt gcccagcccc cgggagacat
     1441 cagatgagta tcttcaccag ctttcagacc cgttacgaaa cgacgcggga agaggaaatg
     1501 tccgtccagg aatacctgga actgtgcaaa tccgacaaga tggcttacgc cagttcggcg
     1561 gagcggatgt tgcaggcaat cggggagccg gagcttgtcg atacgcgtct cgacccccgt
     1621 ctttcgcgaa tcttttccaa caagatgatc aagatctatc ctgccttccg ggatttctac
     1681 gggatggagg aatcgatcga gaacatcgtg tcgtatttcc gacatgcggc ccagggtctg
     1741 gaggaaaaga aacagatcct ctacctgctc gggccggtgg gcggcggcaa gagctccctc
     1801 gccgaaaagc tcaagtcact ggtcgaaaag atccccttct atgccatcaa gggctcgccg
     1861 gtgcacgaat cgccgctcgg gctgttcag
//
LOCUS       DAOQEW010000403         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_38376_length_1887_cov_8.174127, whole genome shotgun sequence.
ACCESSION   DAOQEW010000403 DAOQEW010000000
VERSION     DAOQEW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38376_length_1887_cov_8.174127"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /gene="metG"
                     /locus_tag="PLS35_20550"
     CDS             complement(<1..919)
                     /gene="metG"
                     /locus_tag="PLS35_20550"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNI21117.1"
                     /translation="MTNSARTILVTSALPYANGPIHLGHLVEYIQTDIWVRFQRMRGN
                     ECHYVCADDAHGTPIMLRAQNEGISPQTLIDRVGTEHRQDFSGFQISFDNYYSTHSEE
                     NRHFANLIYERLAKAGHIEQKTIRQAYDPEKRMFLPDRFIKGECPRCGAADQYGDSCE
                     VCGATYSPTDLINPRSALSGATPIEKESEHFFFRLADFEAILKTWIEDGRIQEEIANK
                     LAEWFRDGLQSWDISRDAPYWGFEIPGTPDKFFYVWLDAPIGYMASFKNLCARSGLDF
                     DQYWLPDSSTELYHFIGKDIAYFHTLFWPA"
     gene            complement(1076..>1887)
                     /locus_tag="PLS35_20555"
     CDS             complement(1076..>1887)
                     /locus_tag="PLS35_20555"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNI21118.1"
                     /translation="KKQIAVALGAALALGGTSLAQAGSFLTATNFTGDFVIDQFGVDT
                     AGPTTPDADHTFRIQLSNLDGTVSVQVPPPGYYGVWAEAGFAAGIDYDGVPGQDLGVS
                     YPTTTSLYSGPLTVGNTTTSELLFDFNGTGTTTFTLDGVSQTIPYTTGNITLNGAGAT
                     TFFAALLGIPSFLVGAVTGSVEIDYSVTQDGMEIFIDETNLVGGSFENAILALDNLTA
                     PNGFGLPAGNRNGIIDGTLAANGTIHIPEPASVALLGLGLAGLASRRRKTV"
BASE COUNT          385 a          510 c          622 g          370 t
ORIGIN      
        1 tggccggcca gaacagggtg tggaaatacg caatatcctt gccgatgaag tgatacaact
       61 ccgtgctgga atccggtaac cagtactgat cgaaatcaag accgctgcga gcgcaaagat
      121 tcttgaagct cgccatgtag ccgatcggag cgtccagcca cacataaaag aatttgtccg
      181 gggtgccggg gatttcgaag ccccagtatg gtgcatcacg ggagatatcc catgattgca
      241 ggccgtcgcg gaaccattcc gccagtttgt tggcgatttc ttcctgtatg cggccgtctt
      301 cgatccacgt cttcaggatc gcttcgaaat cagcgagtcg gaagaagaag tgttcagact
      361 ccttttctat gggcgtcgct ccggatagtg ccgagcgcgg gttgatcagg tcggtcgggg
      421 aataggtggc gccgcaaact tcgcaggaat cgccatactg atcggccgct ccgcagcgcg
      481 ggcattcgcc cttgataaaa cgatccggga gaaacatcct tttttcggga tcgtaagcct
      541 ggcggatggt tttttgctcg atatgtccgg ctttcgcaag cctctcatag atcaggttgg
      601 caaagtgacg gttttcttca gaatgtgtcg agtaataatt atcaaaactg atctgaaagc
      661 cgctgaaatc ctggcggtgt tcggtgccaa cgcgatcgat cagtgtctgc ggcgaaatgc
      721 cttcattctg cgcgcgcaac atgatcggtg tgccatgggc atcatcggcg cagacataat
      781 gacattcgtt gccgcgcatg cgctggaagc gaacccagat gtcggtctgg atgtactcga
      841 ccagatgacc cagatggatc gggccattgg cgtaaggcag agcgctggtg acgaggatgg
      901 tgcgcgcgga gttcgtcatg tctggcggtc atgctgcggg aggcgagacc gaccaactta
      961 gcaatatcgg cgaggtatgg ctaccgcagg cagggggcag ggcgtggggc gggcagcaga
     1021 tcaataaaaa acggcggctt gcgccgccgt ttccgggttc agcaattctt ggagatcaga
     1081 cagtcttgcg acgacgcgaa gccaggccgg cgagacccag gccgagcagc gcgaccgagg
     1141 ccggctccgg aatgtggatg gtgccgttgg cggccagcgt gccatcgatg atgccgttgc
     1201 gattgccggc cggcagaccg aacccgttcg gagcggtcag gttgtcgagc gcaaggatgg
     1261 cgttctcgaa gctgccaccg acgagattgg tctcgtcaat gaaaatttcc atgccgtcct
     1321 gggtaaccga gtagtcgatc tcgaccgagc cggtcacggc cccgaccagg aacgacggaa
     1381 tgccgagcag ggctgcgaag aaggtcgtcg cgccggcacc gttcagcgtg atgttgccgg
     1441 tcgtgtacgg gatcgtctgg ctgacgccgt ccagcgtgaa ggtggtcgtg ccggtgccat
     1501 tgaaatcgaa cagcagctcg gaggtcgtcg tgttgccgac cgtcagcggg ccggaataca
     1561 ggctggtcgt ggtcgggtag ctgacgccga gatcctggcc ggggacaccg tcataatcga
     1621 tgcccgcagc gaaaccggcc tcggcccaga cgccgtagta acccggcggc ggcacctgaa
     1681 cggacacggt gccgtccagg ttgctgagct ggatgcggaa ggtgtggtcg gcatcggggg
     1741 tcgtcgggcc ggcggtatcc acgccgaact ggtcgatcac gaaatcgccg gtgaaattgg
     1801 tcgcggtgag gaaagaaccg gcctgcgcga ggctcgtgcc tccgagcgcc agagcggcgc
     1861 cgagcgcgac cgcaatctgc ttcttca
//
LOCUS       DAOQEW010000404         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_39576_length_1858_cov_9.410427, whole genome shotgun sequence.
ACCESSION   DAOQEW010000404 DAOQEW010000000
VERSION     DAOQEW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39576_length_1858_cov_9.410427"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..820
                     /locus_tag="PLS35_20560"
     CDS             314..820
                     /locus_tag="PLS35_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNI21119.1"
                     /translation="MKHAGMTRLALMVGILGFGFGVVGCGGGGEGGGEGPVVPPPPAP
                     AEYADKHMPAGWWTDPKILEEGKELYIGGKNPDVNCASCHGKDGKPVKAGARDFRNGD
                     RMKMYSDSVWFWRISEGVPNTKMKPWKSKLSEEDRWKILAFERSFGLSGKGWDANKKD
                     WVPADQVK"
     gene            886..>1858
                     /locus_tag="PLS35_20565"
     CDS             886..>1858
                     /locus_tag="PLS35_20565"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNI21120.1"
                     /translation="MKIWQRCLLVMFALVAVLGGVAYAQAPSAPPVEFPYTGNRTAVW
                     IVAQLHILFAGFILGAPIFVVISEWLGYRKQDPRYDRLAKEVTKVTVILYSMTALTGG
                     LFIFVLLATYPQFTTWLINHFFLIFAVVYPLLFIGETIVLYMYFYTWDAWKGEKKGRH
                     IALGVLLNLIGSITLFVIDAPTSFMNTPVRAEGISPAEFLATASLWDKVFNYSWMPLN
                     LHRLVGNVTFGGFVAGLIAAYMFMGSKKEEDRAYYDWMGFVGNMIGVGALLFLPFMGY
                     LLAYELCDYDASICPYMMADQLSMFFEMQGAMIGLIFLASNYYIWLSM"
BASE COUNT          359 a          443 c          588 g          468 t
ORIGIN      
        1 gcggctgttg ctattgtggg atggattggg ctaaagatct cccgcaagcg gatgcgagaa
       61 gagcagcggc gaaagaaggg acagggcaag cggcagaaat agggcacggt ccgtggggca
      121 ttgttcctcg ccggatcgaa tagcaagaag taggcgggga agtccaaacg tgacctcgtt
      181 gtattgtgag tcttgacgct tttgctgtgg ctgctcgtgg cgccgcaggg aagcgtcttt
      241 tttttctgtc gatggcaata ggtgtctgcg cgtgaagcgg cgcagacatg aagaaaggag
      301 cgggaacttt tttatgaaac acgcaggcat gacgagattg gctctgatgg tcggcattct
      361 tgggtttggt tttggcgttg tcgggtgcgg cggcggcggt gaaggcggcg gcgagggtcc
      421 ggttgttccg ccaccccctg ctcctgccga gtatgccgac aagcacatgc ctgccggctg
      481 gtggacggat ccgaagatcc ttgaagaggg caaggagttg tacattggcg gaaagaatcc
      541 tgacgtcaat tgcgcgagct gccatggtaa ggatgggaag ccggtcaagg ccggtgcccg
      601 cgacttccgt aacggcgatc gtatgaagat gtattcggat tccgtctggt tctggcgcat
      661 ctccgagggc gtgccgaata cgaagatgaa gccgtggaag agcaagttgt ccgaagagga
      721 tcgttggaag attttggcgt ttgagcgctc gttcggcttg tccggcaagg gttgggatgc
      781 caataagaag gattgggtgc ctgctgacca ggtgaagtaa ttcgcctctg ggttagttcg
      841 agtcatgggt caatcggtag gaatgaggag gagcggcgag acatcatgaa aatctggcag
      901 cgctgtttgt tggttatgtt cgcgctagtg gcggtattgg gaggagtggc ctatgcccag
      961 gctcccagtg cgccccccgt ggagttccct tacaccggga atcgcacggc ggtatggatc
     1021 gtcgctcaac tccacattct ttttgccggg tttattttgg gcgctccgat tttcgtcgta
     1081 atttcggagt ggctgggata tcgcaagcag gatccacgct acgaccggtt ggcgaaagag
     1141 gtcacgaagg tcacggtcat cctgtacagc atgacggcct tgaccggcgg actctttatc
     1201 ttcgtgctgc ttgcgaccta tccgcagttc acgacctggc tcatcaatca cttcttcctg
     1261 attttcgccg tcgtctatcc gctcttgttc atcggtgaaa cgatcgttct ctacatgtac
     1321 ttctacacat gggatgcctg gaagggtgaa aagaagggcc gccatattgc attgggcgtg
     1381 ctgctcaatc tcatcggctc gatcaccctg tttgtgatcg atgcgccgac ctcgttcatg
     1441 aacactccgg tccgggctga aggtatttcg ccagccgagt ttttggcgac tgcctctctc
     1501 tgggacaagg tgttcaacta cagctggatg ccgctcaatt tgcaccgcct cgtcggaaac
     1561 gtcaccttcg gtggattcgt ggctggtctc attgccgcct acatgttcat ggggtccaag
     1621 aaggaagagg atcgtgccta ttacgactgg atgggctttg tcggcaacat gatcggtgtg
     1681 ggtgcgttgt tgttcttgcc gttcatgggg tacttgctgg cctatgagct ctgcgactac
     1741 gacgcgtcca tctgtccgta catgatggct gatcagctgt cgatgttctt cgagatgcaa
     1801 ggggccatga tcggtctcat cttcctcgcc agtaattact acatctggtt gagtatga
//
LOCUS       DAOQEW010000405         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_40827_length_1828_cov_6.314157, whole genome shotgun sequence.
ACCESSION   DAOQEW010000405 DAOQEW010000000
VERSION     DAOQEW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40827_length_1828_cov_6.314157"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLS35_20570"
     CDS             complement(<1..530)
                     /locus_tag="PLS35_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21121.1"
                     /translation="MLKLPMFITRWVGRFCFIMLTAAFFMLFSKQANALVIDYGVRVY
                     AQYYASEIQSSCHSGILLLGCYVTYSEAPNDEVFKDFSAGQPLPYPGIGYESRIEVTA
                     EAREYPVHTTSTGIANFSVLHYHKLITALAYQDGGRTIRDEIPTGDGGALIVMASAES
                     YASILIRLSETLLFTIP"
     gene            complement(798..1790)
                     /locus_tag="PLS35_20575"
     CDS             complement(798..1790)
                     /locus_tag="PLS35_20575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNI21122.1"
                     /translation="MRLPCLLTDLAIALDRFEELERSAGTAAAGFHAVAAAVHTLCTC
                     IAAAEAVGVDRNTLRMLLAPAWEMHGRSPFIHRLQTWPRGYPGDFETIEYLLAQDIRA
                     RPDSVEYWLEFLALQSPIAQQHRNKVRAQGREIAAAALRGTADRPARILILAAGSSPD
                     LALVQHELGPAKCEIVLNDSDEQALAFSLARLAGIRDRLHPLAGNAVKSIPRLRAQGP
                     FDLVLAGGLFDYLPERHAIFLIRTICNELLAADGRLFLTNLASPNPYRVWIEYLADWR
                     LIERSAEELRGLVSAACDPEVSCGLSREGSGMTWLMMIGKGGLMHRLTRISVSH"
BASE COUNT          427 a          533 c          485 g          383 t
ORIGIN      
        1 gggattgtga atagcaatgt ttcagacaat ctaatcagaa tagaagcata agactctgcg
       61 ctcgccataa caatcaaagc ccctccgtca cccgtaggga tttcgtctct aatagttcgc
      121 ccgccatctt gataggcaag tgctgttatt agtttatgat agtggaggac tgaaaaattg
      181 gcaatcccag ttgatgtcgt atgaacaggg tattctctag cttctgccgt gacttcgatt
      241 cgactttcat atccaatgcc tggataaggc aagggctgtc cagcactgaa atctttgaat
      301 acttcatcgt ttggggcttc actatacgtg acgtagcatc ccaacaaaag tatgcctgaa
      361 tggcatgagc tttggatttc gctggcgtaa tactgggcat aaaccctcac cccatagtca
      421 attaccaatg cgttagcttg ttttgaaaac aacataaaaa aagcggctgt caacataata
      481 aaacaaaacc tgcctaccca tcttgtgatg aacatgggca acttgagcat taccggaccc
      541 tctctgttat tttgtaacta aacaaacacc caacaggtgt tgcaacccta ctgcgcaccg
      601 ctttttcctt gcaatgaact caggcgctct ctccacatca accgatagcg atcattgcct
      661 acgctcgaca catgccgcca agagactcat gcagaacgca acctatctcc cttaggccat
      721 caaacccatg tttttacaca caagtctgca acctgttgat tttactagag tgggacgaaa
      781 ttcagcactc atataaatca atgacttaca gagatgcgtg taaggcgatg catcaaacca
      841 cctttgccga tcatcatcaa ccacgtcatg ccggagccct cccggctcag tccgcaactg
      901 acttccggat cgcaggccgc cgacaccaac ccccgcaatt cctccgccga ccgctcgatc
      961 agtcgccaat ccgccagata ttcgatccag actcgatacg ggttcgggct cgcgaggttg
     1021 gtcaggaaca ggcggccgtc cgcggccagc aactcgttgc agatcgtccg gatcaggaag
     1081 atcgcgtggc gttcgggcag atagtcgaac agccctccgg cgaggaccag atcgaacggg
     1141 ccctgtgccc gcaggcgggg aatcgatttc actgcgttgc cggccagcgg atgcagtcgg
     1201 tcgcgaatgc cagcaagtcg tgccagcgag aacgccaagg cctgttcgtc gctgtcgttc
     1261 agcacgattt cgcacttcgc cgggccgagt tcgtgctgca cgagtgccag atcggggctg
     1321 ctgccggcgg ccagaatcag gatgcgggcg ggacgatcgg cggtgccgcg cagcgctgcc
     1381 gccgcgatct cgcggccctg ggcacgaacc ttgttgcggt gctgctgggc aatcggcgat
     1441 tgcagcgcga ggaattccag ccagtactcg accgaatccg gacgtgcccg gatgtcctgc
     1501 gccagcagat actcgatcgt ctcgaaatcg cccggatagc cgcgtggcca ggtctgcaga
     1561 cggtggatga acggcgaccg cccgtgcatc tcccaagccg gtgccagcag catgcgcagc
     1621 gtgttgcgat cgacgccgac ggcttcggcg gcggcgatgc acgtgcagag cgtgtggacg
     1681 gccgccgcga cggcatggaa accggccgcc gcagtgcctg cgctgcgctc aagttcttcg
     1741 aaacgatcaa gggcgatggc gagatcggtg aggaggcagg gcaaacgcat ctaaatcatc
     1801 tgcccgttca acaccttgat cagatagc
//
LOCUS       DAOQEW010000406         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_41216_length_1819_cov_10.058957, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000406 DAOQEW010000000
VERSION     DAOQEW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41216_length_1819_cov_10.058957"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1160)
                     /locus_tag="PLS35_20580"
     CDS             complement(153..1160)
                     /locus_tag="PLS35_20580"
                     /inference="COORDINATES: protein motif:HMM:NF017518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HNI21123.1"
                     /translation="MIELYEGVPGSGKSYHAICEKFLPWVKQGRRLYIAVDGIYLDRL
                     SLFTGIELSTLEQQITIWKDSVEVLQAFPHVEPGSAVIIDEAQTVFRSMQKVEPGLLR
                     WLETHRHYGVDILLMSQDFRQMSQGVTRLIEATVKFRRLAFVGLSKKYQGKVRGNPED
                     HEVIRAFVGTYSPAIYAYYSSYASAAIREEKRSHTVFKSARVAIGIAAGLFAIGLMVW
                     RPWSSLSASKPVSSGGSTPAATTTIGAPVSGSATLLNPLGMSSPTATSRPVPKRPVRI
                     LGGAGMSKDRQGWRYLLDSGEILTAAQITGRYGLMVSEVYEDGSMRLIGEGVFYGPAG
                     D"
     gene            complement(1240..1515)
                     /locus_tag="PLS35_20585"
     CDS             complement(1240..1515)
                     /locus_tag="PLS35_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HNI21124.1"
                     /translation="MTAILTLIYCWLQEFFFSLTDWGLGIWDSLLSVADSTLATIGTA
                     GLTLPVIPDQYAWVLGATGMSQALAIVASAMGTRFILQTIPFVRWGS"
     gene            complement(1512..>1819)
                     /locus_tag="PLS35_20590"
     CDS             complement(1512..>1819)
                     /locus_tag="PLS35_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21125.1"
                     /translation="ETQATASCTVGSHESRTFGTVLQAHQTIWASSGLLGTLNLLKSL
                     TWPSTLPVIALPSAFFGSQQVDFNQWAWFFTVLRTLVIATASIAAYRIIFVGGGQTT"
BASE COUNT          420 a          538 c          515 g          346 t
ORIGIN      
        1 tccttgtgct ggtggtggtt ccgtccaccc acgccgccgg atgactcgcc ggcggacgag
       61 cgcctcagta agtctgaggc agaatcaatt tgatgatcag gccgacggcg aagccgccga
      121 gccaaaagat cgcggtgaaa taggtcatcg cttcaatcgc cggcaggtcc atagaacact
      181 ccttcgccaa tgagccgcat tgagccatct tcatagactt ccgagaccat caggccatag
      241 cgtccggtaa tttgagcggc cgtgaggatc tcgccactgt ccaggagata gcgccagcct
      301 tgacggtcct tactcattcc cgctccgccg aggattcgaa cgggtcgctt cgggacaggc
      361 cgagaagttg ctgtcggaga agacatgccc aagggattca gcagagtcgc cgaaccggag
      421 actggtgcgc cgatggtggt ggttgcggca ggagttgacc cgccggaaga gactggcttg
      481 ctcgcactta acgacgacca tggacgccaa accatgagac cgatggcaaa cagtcctgcg
      541 gcaatgccga tcgcgacccg cgccgactta aagacggtat gactgcgctt ctcttcccga
      601 atggctgccg acgcataact ggaatagtag gcatagatcg ccggcgaata cgttccgaca
      661 aaggcccgaa tgacttcgtg atcttcgggg ttcccacgca ccttaccttg atacttcttc
      721 gagaggccga caaaggccag cctccgaaac ttcaccgtgg cttcaatgag tcgcgtgaca
      781 ccttgggaca tctgccgaaa atcctggctc atgagcagaa tgtccacccc gtaatgacga
      841 tgggtttcga gccagcgcag cagacccggt tccacttttt gcatcgaccg aaacacggtt
      901 tgcgcttcgt cgataatcac cgccgatccc ggttcgacat ggggaaacgc ttggaggact
      961 tccacggaat ccttccagat ggtgatctgt tgctcgaggg tagacagttc aatacccgtg
     1021 aacagggaaa gacgatctaa atagatccca tcgacggcga tatagagccg tcggccttgt
     1081 ttcacccagg gcaagaactt ctcgcagatc gcgtgatacg atttccctga gcccggcacc
     1141 ccttcataca gttcgatcat cgaagactcc tgagatacac ccagatccag agagctaaaa
     1201 tcaccgtcca ggcataggcc cagcccatca ccagttggtt catgagcccc accggacgaa
     1261 cggaatagtt tgcaaaatga atcttgtccc catggcgctc gccacgatgg ccagcgcctg
     1321 actcatgccg gtcgcgccca gcacccacgc atattgatcg ggaatcaccg gcagggtgag
     1381 ccctgccgtc ccaatggtgg cgagcgtgct gtccgccacg gagagcaggg aatcccagat
     1441 cccgaggccc caatccgtga gggagaagaa gaactcctgt agccagcaat agatgagggt
     1501 cagaatcgcg gtcatgtcgt ctggcctccc ccgacgaaga tgatgcgata ggcggcaatg
     1561 gaggccgtcg caatcaccaa ggtgcgcagg accgtgaaga accaggccca ttgattgaag
     1621 tccacttgct ggctgccgaa gaaggccgac ggtaaggcaa tcaccggcaa ggtcgagggc
     1681 caggtgagcg acttcaagag attcaatgtg ccgagtaacc cactgctggc ccagatggtc
     1741 tgatgcgctt gcaggacagt cccgaaggtc cggctctcat gggaccccac tgtgcatgat
     1801 gccgtcgctt gcgtctctt
//
LOCUS       DAOQEW010000407         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_42270_length_1796_cov_3.767949, whole genome shotgun sequence.
ACCESSION   DAOQEW010000407 DAOQEW010000000
VERSION     DAOQEW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42270_length_1796_cov_3.767949"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="PLS35_20595"
     CDS             <1..1449
                     /locus_tag="PLS35_20595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNI21126.1"
                     /translation="SLAWRPNSRFEGNLDIEYQRNTFTDVSDIGIPAIGNRPAPIPLS
                     RFTGDPAAKNTQRRVLVGLDWTYRFNPDWKIINRFQFNDVAYDQRTLFAVDFDQSTGE
                     LTRGLWHADLNRRTYAANLDLVGHVRTGPIKHDLLVGVDYYRFEGVYSAFAGQTPAVP
                     SFNIFNPSYGIDLSSITRATYNQFGTFPEQWYGVYFQDQITLWDKVHILGGGRYDWAE
                     VGTSFSDTSLALAHAGETKERTGYFSPRVGIVYRPWQWLSLYGNYVKSLGSNNSGTPL
                     PGSGSLDPQTARQWEAGIKTEFFGGRVNSTLAFFDLTKENVATVIPGTPFSRTIGEAN
                     SRGMEFDVSGRITDQLNLIASYAYTDAKVTKDLSGIEGNRLISVPAHSGSLWAVYEFT
                     DGMLQGVRLGTGAFARSSRMADLDNTVELPGYVRWDASAGYTFTYSGSKMTAQLNVYN
                     LLNTDYYDRGNSRLVIQPGLPLTFLGSLRIEM"
     gene            1517..>1796
                     /locus_tag="PLS35_20600"
     CDS             1517..>1796
                     /locus_tag="PLS35_20600"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNI21127.1"
                     /translation="MSTRRAGLFTRPFWVRLHRWAGLTMAGFLIVVGLTGSLLAFYPE
                     LERLMHPHWYPDRAPATWMGAGVSAEKLERAEPRLRVTQVSLQGFDGAT"
BASE COUNT          362 a          526 c          531 g          377 t
ORIGIN      
        1 tccctcgctt ggcggcccaa tagtcgattt gaaggtaact tggatattga ataccagcgc
       61 aatacgttca cggatgtcag cgatatcggc attcccgcta ttggcaatcg tcccgctcct
      121 attccgctga gccggtttac cggcgatcca gcggccaaaa acactcaacg acgtgtcttg
      181 gtgggactgg attggaccta ccgattcaac cctgattgga agatcatcaa ccggtttcag
      241 ttcaacgacg tcgcctacga tcaacgcacg ctgtttgccg tagacttcga ccagagcacg
      301 ggggagctga cccgggggtt gtggcatgcc gatctgaacc gcaggaccta tgccgccaat
      361 ctggaccttg ttggacatgt ccgcacgggg ccaatcaagc acgatctgct cgtcggcgtg
      421 gactattatc gatttgaagg cgtctatagc gcctttgccg ggcaaacgcc cgccgtccca
      481 tcgttcaata tcttcaatcc atcatacggg atcgacctct cctcgattac ccgcgccacc
      541 tacaatcaat tcggcacgtt tcccgagcag tggtacggcg tgtacttcca agatcagatt
      601 accctgtggg acaaggtgca tattctcggc ggcggtcgct acgactgggc cgaggtcgga
      661 accagttttt ctgacacctc attggccttg gcgcatgccg gtgaaaccaa agagcgcacc
      721 gggtatttca gcccgcgcgt gggcatcgtg tatcggccat ggcagtggct ctctctctac
      781 gggaactatg tcaaatcctt gggaagcaac aacagcggca cgccattgcc gggtagcggg
      841 tcgcttgacc ctcaaacggc cagacagtgg gaagccggca tcaaaaccga attcttcggc
      901 ggcagagtca attcgacctt ggcgttcttc gacctgacca aggagaatgt ggcgaccgtg
      961 attccgggca cgccgttcag ccggacaatc ggagaggcca acagtcgtgg catggaattc
     1021 gatgtgtccg gccgcatcac cgaccaactc aacctcatcg ccagttacgc ctacaccgac
     1081 gcgaaagtga ccaaagactt gagtggtatc gaagggaacc ggctcatttc ggtgcctgcg
     1141 cattccggaa gcctctgggc ggtctacgaa ttcaccgatg gcatgctgca gggagtccgt
     1201 ctcggaaccg gggctttcgc acggtccagc cggatggcgg atctggacaa tacggtggag
     1261 ttgcccggct atgtccgatg ggatgcgagc gccggataca ccttcacgta ttccggatcg
     1321 aaaatgacgg cccagctgaa tgtgtacaac ttgctcaata cggactatta cgaccgcggc
     1381 aattcccggc ttgtcattca accgggcttg ccgctgacat tcctgggttc gcttcgcatc
     1441 gagatgtgac gcaggcaggg cattcccgcg aatcggcgag gctcgtgcct gatggtggtc
     1501 tcaggaggtg agaggaatgt cgacacgccg cgcagggctg ttcaccagac cgttctgggt
     1561 tcgactgcat cgctgggccg ggctgacgat ggcgggattc ttgatcgtgg tcgggctgac
     1621 cggaagtctg ctcgcgtttt atccggagct cgagcggctc atgcatcccc actggtatcc
     1681 ggaccgcgcc cccgccacgt ggatgggcgc cggcgtgtcg gcggagaagc tggagagggc
     1741 cgagccgcga ctgcgcgtca cgcaggtgtc tcttcaagga ttcgacggcg cgacct
//
LOCUS       DAOQEW010000408         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_42563_length_1789_cov_8.335063, whole genome shotgun sequence.
ACCESSION   DAOQEW010000408 DAOQEW010000000
VERSION     DAOQEW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42563_length_1789_cov_8.335063"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..924)
                     /locus_tag="PLS35_20605"
     CDS             complement(94..924)
                     /locus_tag="PLS35_20605"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI21128.1"
                     /translation="MSALTAKQRTGTFIVTKEHRRFCEFANAVRKYRYIGLCYGPAGV
                     GKTLSAMRYAHWDIVEPLVQSWGPRSPTDARVYAAAARARTVFYTPLVGETPRRLREE
                     LSLLISRTGICIEQHQDPASANPTHQYNEYVEMIIIDEAERLTALALESVRDLFDRTG
                     IGVVLIGMPGIEKRLSRYEQLYSRIGFAHHYRTLQGDELSFVLTRHWRKFGLDLDGAD
                     FTDAQAMAAIARITGGNFRLLHRLFVQIDRILRINELNVITSDVVEAARSTLVIGTAT
                     "
     gene            complement(921..>1789)
                     /locus_tag="PLS35_20610"
     CDS             complement(921..>1789)
                     /locus_tag="PLS35_20610"
                     /inference="COORDINATES: protein motif:HMM:NF020859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HNI21129.1"
                     /translation="YTVFLGSPSALQTSLALRQAIWRKPDPQWPVCGIPDILYTDHGS
                     DFTSQHLEQVAADLRMELVHSIPGRPQGRGKVERLFGSLNTELLPELPGHLMKGKPVT
                     PPRLSLTELDAAIQTHIIGTYHVRTHGETGVPPREAWIAQGWLPRLPECVEDLDLLLV
                     MVAKARIIHRDGIHFQGMRYLSPTMAAYVGEEVTIRYDPRDISEIRVFHRHRFLCRAI
                     SPEHASQTITLKDIQTARNAYRRAVRGHIRERIARVTDFLPAPESKTPESPALSGKVQ
                     PPGLLTYYEDKP"
BASE COUNT          378 a          522 c          530 g          359 t
ORIGIN      
        1 tcgttccgag agtggtttgc gagaatcgca aagccttgcg gcacagtagc ctgcttgtga
       61 tcttcagagc taattggcgc aaaatcttca gcactatgtg gcggtgccaa tgacgagcgt
      121 gctgcgcgcc gcttcgacga catcactggt aatgacgttt aattcgttga tacgcaggat
      181 gcggtcgatt tgcacaaaca gccggtgcag aaggcggaag ttgcccccgg tgatacgggc
      241 tatggccgcc atcgcttgtg catccgtaaa atcagccccg tccaggtcca ggccgaactt
      301 gcgccaatgc cgggtcagca caaatgacag ttcatcgccc tgtaaagtcc gatagtggtg
      361 cgcgaacccg atgcggctgt agagctgttc gtagcgcgac aggcgttttt caatccccgg
      421 catgcctatc agcacaacgc caatgcctgt gcggtcaaac agatcgcgca cggactccag
      481 agcgagggcc gtcaaccgct cggcctcgtc gatgatgatc atctcgacat attcgttgta
      541 ttgatgcgtc gggtttgccg acgccggatc ctggtgctgt tcgatacaaa tgccggtacg
      601 actgatgagc aacgacaact cttcccgcag acggcgtggg gtttcaccca ccaagggtgt
      661 ataaaacacg gtgcgggcac gggcggccgc agcataaacc cgtgcatccg tgggcgagcg
      721 cggcccccag gactgcacca ggggctccac gatatcccag tgagcataac gcatggccga
      781 caaggtcttg ccgacaccag ccggaccgta gcacagcccg atgtagcggt atttgcgcac
      841 cgcattggca aactcgcaaa atcgacggtg ttccttcgtg acgatgaacg ttccggtgcg
      901 ctgcttcgcg gtaagggcgc tcatggcttg tcctcataat acgtaaggag tcccggtggc
      961 tgcactttgc cggatagcgc cggcgattct ggtgttttgc tctccggcgc gggcagaaaa
     1021 tccgtcactc gggcaatacg ctcccggatg tgtccgcgca cggctcgccg gtaggcgtta
     1081 cgggcagtct gaatgtcctt cagcgtgata gtctggctgg cgtgctcagg actgatcgcc
     1141 cgacacagga agcgatggcg atgaaagaca cgaatctcac tgatgtcacg cgggtcatac
     1201 cggatggtca cctcctcgcc cacataggcg gccatggtcg gcgacagata ccgcatcccc
     1261 tgaaaatgga tgccgtcacg gtgaatgatg cgggccttgg ccaccatgac cagcagcaga
     1321 tccagatctt ccacacactc ggggaggcga ggcagccacc cttgcgcaat ccatgcctca
     1381 cgcgggggaa ctccggtttc accatgtgta cggacgtgat atgtcccgat gatatgggtc
     1441 tgaatggcag catccagttc cgtcagagag agccgcggtg gtgtgacggg tttgcccttc
     1501 atcaaatgcc caggcagttc gggaagcagc tctgtgttca gcgaaccaaa caggcgctcc
     1561 accttgccac gcccctgcgg tcggccgggg atggagtgca ccagctccat ccgtaaatcg
     1621 gcagcgacct gctccaaatg ctgactggtg aagtcactgc catggtcggt atacaggatg
     1681 tccggaatac cgcataccgg ccactgagga tcgggttttc gccaaatcgc ctgacgtaag
     1741 gccagtgatg tttgcagcgc actgggcgat cccagaaaca cggtataac
//
LOCUS       DAOQEW010000409         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_44341_length_1752_cov_6.999411, whole genome shotgun sequence.
ACCESSION   DAOQEW010000409 DAOQEW010000000
VERSION     DAOQEW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44341_length_1752_cov_6.999411"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..335)
                     /gene="dut"
                     /locus_tag="PLS35_20615"
                     /pseudo
     CDS             complement(105..335)
                     /gene="dut"
                     /locus_tag="PLS35_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
     gene            complement(349..1518)
                     /locus_tag="PLS35_20620"
     CDS             complement(349..1518)
                     /locus_tag="PLS35_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNI21130.1"
                     /translation="MNAGKTLFAQLMDFLPWSTFHRYVTRYGGDKGVRTMTCAEQFRV
                     MAFAQLTYRESLRDIEASLSAQAAKCYHMGFREPVRRSTLADANESRDWRIYADFAAR
                     LIVQARTLYAGEDLGLELSNTVYALDSTTIDLSLSVFPWAHFRTTKAAVKMHTLLDLR
                     GSIPSFIHVSDGKLHDVHALDLLTPEAGAIYVMDRGYVDFARLHRLHLAGAFFVTRAK
                     SNLKAHRVYSAQTDRSTGILCDQTIALDGFYSKQDYPAQLRRVRFNDAETGKTLVFLT
                     NQMTLPAAIICALYKSRWQVELFFKWIKQHLRIKQFFGTSENAVKTQIWIAVSVYVLV
                     AIVRKKLNLSLSLYTLLQVLSLTLFEKMPLQQAFPGNDHTSDNGSISNQLNLFNI"
BASE COUNT          403 a          496 c          509 g          344 t
ORIGIN      
        1 gtagagacca tcgaaaaagc cggtgccagc gcggcgcatg agcaacacgc ggccgtcccg
       61 ctccagcagg acatgcacgc cggtagggat gcccgcatgg cgcatcagtg cttgcccgtg
      121 ctgccgaaac cgccttcgcc acgatcgctc tcgtcgaagg attcgaccac gttgaagccc
      181 acctggagca ccggcaccac caccagctgg gccacgcgct ccatcggctg gatggtgaag
      241 tggtcgtggc cacggttcca gacggacacg aagatctggc cctgataatc cgagtcgatc
      301 aacccgacga gattgcccag cacaataccg tgcttcactg ctgtccggtt aaatattgaa
      361 tagattcaat tggttgctga tgctgccatt gtccgaagtg tggtcgttgc ccggaaacgc
      421 ttgctgcaag ggcatcttct cgaacaaggt gagggataga acctgtagca aagtgtagag
      481 ggaaaggctg aggttcagtt tttttcgaac aatggcaacc aggacataga ctgacaccgc
      541 gatccagatt tgcgtcttga ccgcattttc cgacgtaccg aagaactgct tgatacgaag
      601 atgttgcttg atccacttga agaacagctc gacctgccag cggcttttgt aaagcgcgca
      661 gatgatcgct gccggcaagg tcatctgatt ggtgagaaag acgagtgttt ttccggtttc
      721 cgcgtcgttg aaacggaccc gacgcaactg cgcggggtag tcctgcttcg agtagaagcc
      781 atcgagtgca attgtctgat cgcaaaggat gccggtactg cggtctgtct gtgccgaata
      841 aacccgatgc gccttgagat tcgatttggc gcgcgtgacg aagaaggcgc ccgccagatg
      901 caatcgatgc agacgagcaa aatcgacata cccgcgatcc ataacgtaga tcgcaccggc
      961 ttccggagtc agcaaatcca gggcatgcac gtcgtgcaac ttgccgtcgg agacatggat
     1021 gaaactcgga atgctgcccc gcaagtcgag cagcgtgtgc atcttgaccg ctgccttggt
     1081 ggttcgaaag tgcgcccacg gaaacaccga cagagacaga tcgatggtgg tcgaatcgag
     1141 ggcatacacc gtgttcgaca attccagccc caagtcttcg ccggcataga gcgttctcgc
     1201 ttggacgatc agacgggcag cgaagtcggc atagatgcgc cagtcgcgcg attcgttggc
     1261 gtcggccagc gtcgagcgcc tgacgggttc gcgaaacccc atgtgatagc acttggcggc
     1321 ttgcgcagaa agactggcct cgatgtcgcg cagactttcc cggtaagtga gttgagcaaa
     1381 ggccatcacc cggaactgtt ccgcacaagt catcgtgcgg acacccttgt cgccaccgta
     1441 ccgcgtcaca tagcgatgaa atgtcgacca cggcagaaaa tccatcaact gggcaaacag
     1501 tgtcttgccg gcattcatga tcacgccctt cagaaaaatc cgaagggtca tttttcgccg
     1561 atttcaaatc gacaccacgc ttcatcgctc ggaaagcctc gtcagtcatg gcgtttggcg
     1621 gttcgggcag tcatttaacc ggacagtagt gagtagaggt catgggacag ggcgtgggag
     1681 atcgtcagca gcaggccgtc ggcggtcgat agcgccgcag ccaagccgcc cgcggccacc
     1741 agcccggaca cc
//
LOCUS       DAOQEW010000410         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_45954_length_1719_cov_6.399038, whole genome shotgun sequence.
ACCESSION   DAOQEW010000410 DAOQEW010000000
VERSION     DAOQEW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45954_length_1719_cov_6.399038"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLS35_20625"
     CDS             <1..393
                     /locus_tag="PLS35_20625"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HNI21131.1"
                     /translation="AVTTRHYLADAAFLVGLCGDDRGLLERIHGALADPVWPLALGRK
                     SYVPSEPIWMPDAVVDADMRDTLVQWPAIASPRPGAPPTPRILSLESADRSGLMKMDQ
                     PLSSFDERRFGPRFVVSEVLDVPVQTGP"
     gene            368..1000
                     /gene="cas6e"
                     /locus_tag="PLS35_20630"
     CDS             368..1000
                     /gene="cas6e"
                     /locus_tag="PLS35_20630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HNI21132.1"
                     /translation="MFLSKLVLDPAHPQARRDLANPYEMHRTLSRVYAESPDGPPARF
                     LWRLEHTGNHLPEDGTTVLLQSATPGRWRCLQELTGYLHALYPDKAVALDELLQPGRS
                     YVFRLCCNPTVTRNGKRYGLLREEEQVAWLARQGRQHGFTPLHARIVRSEHATHHQGR
                     GGQRITLQVVQFDGLLRAEDPAQLGQALVNGIGHAKAMGQGMLSIAPGHG"
     gene            1003..1644
                     /gene="cas1e"
                     /locus_tag="PLS35_20635"
     CDS             1003..1644
                     /gene="cas1e"
                     /locus_tag="PLS35_20635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HNI21133.1"
                     /translation="MLPPLKPIPIKERLSIVFVEKGEIDVVDGAFVVVDQQGFRVRPV
                     LTHIPVGGVACIMLEPGTRVSHMAAALAARVGTLLVWVGEAGVRLYSSGQPGGARADR
                     LLYQARLALDEALRLKVVRKMYAMRFGDEPPARRSVEQLRGIEGARVKRTYQLLAQKY
                     GVKWTGRAYNPEDWDVSDLPNRCLSSATAALYGVTEAAVLVSVVTRFDVPMIT"
BASE COUNT          288 a          563 c          550 g          318 t
ORIGIN      
        1 gccgtgacca cccgccatta cctcgccgac gcggccttcc tggtcggcct gtgcggcgac
       61 gaccgcggct tgctcgaacg catccatggc gccctcgccg atccggtgtg gccgcttgcc
      121 ctgggacgca agtcctatgt gccctcggag cctatctgga tgcccgatgc ggtagtggat
      181 gccgacatgc gcgacacact tgtccagtgg cccgccatcg cctcaccccg cccgggcgcg
      241 ccacctaccc cgcggattct gtccctcgag tccgccgacc gctccggtct gatgaagatg
      301 gatcagcccc tgtcatcctt tgacgagcgc cgttttggcc cccgtttcgt cgtttcggag
      361 gtgctggatg ttcctgtcca aactggtcct tgaccccgct cacccacagg cgcggcggga
      421 tcttgccaat ccctatgaga tgcaccgtac gctgagccgg gtctatgccg agagcccaga
      481 tggcccgccg gcacgcttcc tgtggcggtt ggaacacacc ggcaaccacc taccggagga
      541 tggcaccacc gtactgctgc aatctgcgac gcctggccgc tggcgttgcc tgcaggaact
      601 gaccgggtac ctccatgccc tgtatccgga caaggcggta gctctcgatg agttgctgca
      661 gccgggtcgc agctatgttt tccggctttg ctgcaatcca accgtcaccc gcaacggcaa
      721 acgctacggt ctactgcgcg aagaagaaca ggtcgcctgg ctggcccgcc aaggccgcca
      781 acatggattc accccgctcc atgcccgtat cgtccgcagc gagcacgcta cccatcacca
      841 gggacgtggt ggtcaacgca tcacattgca ggtggtgcaa ttcgatggcc tgctgcgggc
      901 ggaggatccg gcgcaactag gacaggctct ggtgaacggc atcggccatg ccaaagcgat
      961 gggccagggt atgctgtcga tcgccccagg gcacggctga gcatgctacc acccctcaaa
     1021 cccatcccga tcaaggagcg cctgtcgatc gtcttcgtcg agaaaggtga gatcgatgtg
     1081 gtggatggcg ccttcgtggt agtggaccag caaggcttcc gggtgcggcc ggtgctcacc
     1141 cacatcccgg taggcggggt tgcctgcatc atgctagagc ccggcacgcg ggtatcccat
     1201 atggcagctg cactggcggc gagggttggt acgctactgg tatgggtcgg ggaggccggc
     1261 gtacgcctgt attcctccgg ccagcccggt ggagcgcgcg ctgaccgact gctctaccaa
     1321 gcgcgattgg cgctggacga agccctgcgg ctgaaggtgg tgcgcaagat gtacgcgatg
     1381 cgcttcggtg atgagccgcc ggcccgacgc agcgtggaac agctgcgcgg catcgagggg
     1441 gcacgggtca agcgtacgta tcagctactc gcgcagaagt acggcgtgaa gtggaccggt
     1501 cgagcctata acccggagga ctgggacgtc tccgacctgc ccaaccgctg tctgtcgtcc
     1561 gctaccgctg cgctctacgg cgtgaccgaa gccgcagtgc tggtgagtgt ggtcacccga
     1621 tttgatgttc cgatgatcac gtagtgcgat ggcctgcaaa ggcccgccgc cgaatggaaa
     1681 cgccgcccgg tgatgctgcg gggcggcgtt tgtgcttga
//
LOCUS       DAOQEW010000411         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_47782_length_1685_cov_10.388344, whole genome shotgun
            sequence.
ACCESSION   DAOQEW010000411 DAOQEW010000000
VERSION     DAOQEW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47782_length_1685_cov_10.388344"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..587
                     /locus_tag="PLS35_20640"
     CDS             306..587
                     /locus_tag="PLS35_20640"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNI21134.1"
                     /translation="MNQERQASATLEIMTLAEAAVYLRVSKSTLYQRKDIPRHRMPGS
                     REVRFLKDELIAWLKSGDGEAQSADSSELVFPLDTAHTTVYHRNPKYRG"
     gene            590..>1685
                     /locus_tag="PLS35_20645"
     CDS             590..>1685
                     /locus_tag="PLS35_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNI21135.1"
                     /translation="MAYTITKRRTTKGIAFDLYFRWKGQRYRPLLGYDLSPHQAQQCA
                     IDLIHKIQNEEHEKPRSLITLRDVLPLYWQAFEVKKRIDRTRPKGILENHILSCHACV
                     RNDFSCSHRFGHQPLAAIRPEDGLKYVKARLDEGATAGTVRREWQVLNRILNVAVRYE
                     KLDRNPLKHVELPDAEKRTRIAEPEELSLISKMRENDPTKLECRQELWRIIQVAVSVG
                     LREGKILEIERSWMKKREDGYWLCLPPAASRIKGTPQEVPLNRTAMLALSAEIPSLAD
                     GRVFRRWTHQRAFKEYWSETCRRVGIHDLHFHDLRHTFATRLQRIGVDYEVRQALLGH
                     RMPGMTANYSHGGPEWDRKLRSAVEGLEKAY"
BASE COUNT          408 a          481 c          475 g          321 t
ORIGIN      
        1 cgtatttccg aaccgggctc agtggccgtc caccgttcga ggaaccgaga gtactgtcaa
       61 cggacatgtc cgcctccttc tgaaattatg ttctagcccc acccttcacc aaccaagggt
      121 ggggcgtaac gaaggaggcg gcacggggct ctcgcagcgg gcaggcctgg aggggcggag
      181 accggcacgg agggcaccgg gaaggcctgc tccctgctcc ccggccccga gtcgtaccca
      241 ctgggggctc actcttcgac ccaagccgga ctgtgcagag gcgtttcagt gagagcggga
      301 aaagaatgaa tcaggaacgg caagccagcg cgacgctgga aatcatgact ctggccgaag
      361 cggcggtcta tctccgcgtg tcaaaatcca cgctgtatca gcggaaggat attccgaggc
      421 atcgcatgcc gggatcgcga gaagtcagat ttctcaaaga cgagctgatc gcctggttga
      481 aaagtggcga tggagaagca cagtcggcgg attctagcga gctggttttc ccactagaca
      541 cggctcatac cactgtttac catcggaacc caaaatatcg gggatgacca tggcttatac
      601 gattaccaaa cgacgcacga ccaaagggat cgcctttgac ctctatttcc ggtggaaggg
      661 gcaacggtat cgtcccctac tcggctacga cctctcgccc catcaagcac aacagtgtgc
      721 catcgacctg attcacaaga ttcaaaacga agaacacgag aagccgcgtt cgctcatcac
      781 cctccgcgat gtcctcccgc tgtattggca ggccttcgaa gtaaaaaagc gcatcgatcg
      841 cacgcgacca aaaggaattc tggaaaatca catcctgtcc tgccatgcgt gcgtcagaaa
      901 cgacttctcg tgcagtcatc gcttcgggca tcagccgctc gcagccatca gaccggaaga
      961 tggcctgaag tatgtgaaag ctcgtctcga tgaaggggcc acggccggca ccgtccgccg
     1021 tgaatggcag gtcctcaatc ggattctcaa tgtcgccgtg cgctatgaga aattggatag
     1081 aaacccattg aaacatgtcg aactgccgga cgcggagaaa cgaaccagaa tcgcagagcc
     1141 agaggaactt tcactcatca gcaagatgcg agagaacgat cccaccaagc tcgaatgccg
     1201 gcaagaactc tggcgcatta ttcaggtagc cgtctcggtc ggcctgcggg aagggaaaat
     1261 tctagagatc gagcgttcat ggatgaagaa gcgagaagat gggtactggc tatgtcttcc
     1321 tcctgccgca agccgtatca agggcacccc gcaagaagta ccgttgaacc gcacggccat
     1381 gcttgcgcta agtgcagaga tcccttctct cgccgatggt cgagtattcc gccgttggac
     1441 ccatcaacgg gcttttaagg agtattggag tgaaacctgt cgccgggtcg ggattcacga
     1501 ccttcatttt cacgatcttc ggcatacgtt cgcaacccgc cttcaacgca tcggtgtgga
     1561 ttacgaagtc cggcaggcac tccttggcca tcgcatgcct ggcatgacgg cgaactattc
     1621 tcatgggggt ccggagtggg accgaaagct gcgatcagcc gtggaaggac tcgaaaaagc
     1681 ctatc
//
LOCUS       DAOQEW010000412         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_49360_length_1658_cov_7.395508, whole genome shotgun sequence.
ACCESSION   DAOQEW010000412 DAOQEW010000000
VERSION     DAOQEW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49360_length_1658_cov_7.395508"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1410)
                     /locus_tag="PLS35_20650"
     CDS             complement(133..1410)
                     /locus_tag="PLS35_20650"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNI21136.1"
                     /translation="MTLTPHQQAAFDSLMNFASLDSTAGMAVLSGYAGTGKTFLVGKL
                     ISELKQGGVRKLAVAAPTHKALSVLAAKIDTPVDFLTVHSLLGYKMTANEDVKVAERS
                     NPPSLDKFNFVIIDESSMIGAKFFSDIVLHRGWTKVLFVGDPAQLPPIGEPQRSPVFD
                     RVQFQVHLSEVVRQAAENPIIALSMAVRAAHEAGATMDSRAVLSAVPASAFNIGITQR
                     EVLGQWALDEHKDGRDCRILAYTNARVLALNADIHEALHGATPTPFVPGERVIVHEAR
                     DSVRSADHPGVRVSLRTSEELTVRSVEWQKSSFDRYDLHVVAEMDSGACVCGIWLDEE
                     KQRETQRRFSEARVLTDRQEKRHAFQRAFAFRESFLDLRHAYAMTVHKSQGSTFDTAL
                     IDLPDLMRMPPAEFNQALYVAITRPREYLAIGV"
BASE COUNT          329 a          497 c          490 g          342 t
ORIGIN      
        1 cacccggttg gcggacgaca actgctccag caaaccgaat gggtcgtgcg gcatctcaag
       61 cactccgaaa gcccttgcac cgggtacagc cctggatcgc aaaaagaagg cccgcgtgac
      121 tcttcatcgt cgtcaaactc cgatggccaa gtactcccgc ggccgggtga tggccacgta
      181 gagcgcctga ttgaattccg cgggtggcat gcgcatgaga tccggcaggt cgatgagtgc
      241 cgtatcgaag gtgctgccct ggctcttgtg caccgtcatc gcgtaggcgt ggcgcaggtc
      301 cagaaagctc tcgcgaaaag cgaaggctcg ctgaaaggcg tggcgttttt cttggcgatc
      361 tgtcaaaacc ctcgcttcgc tgaaccggcg ctgggtttcc cgctgctttt cctcgtccaa
      421 ccagatgccg caaacgcacg cgccgctgtc catctctgct accacgtgca agtcgtaccg
      481 atcgaacgaa ctcttctgcc actccacaga gcgcacggtc agttcttcgg acgttcgcag
      541 tgacacgcga acaccgggat gatccgcgct gcgtactgaa tctctggcct catgcacaat
      601 gacccgctcc ccagggacga atggcgtcgg cgtggcgcca tgcaatgcct cgtggatgtc
      661 cgcattgagc gccaacacgc gggcattggt ataggccagg atgcggcaat cgcgcccatc
      721 cttgtgctca tcgagcgccc attggccaag tacctcgcgt tgcgtaatgc cgatgttgaa
      781 tgcgctcgct gggaccgccg aaaggaccgc ccgggaatcc atggttgcgc cggcctcgtg
      841 cgctgccctg acggccatgg agagcgcaat gatggggttt tcggcggcct gcctcactac
      901 ttctgacaga tgcacctgga actgcacccg atcgaacacc ggggacctct gtggctcacc
      961 gatcggcggc agttgcgccg ggtcgccgac gaacagcacc ttggtccacc cccgatgcag
     1021 cacgatgtcg gaaaagaact tggctccgat catggatgat tcgtcgatga tgacgaaatt
     1081 gaacttgtcg agggacggag ggttgcttct ttctgccacc ttcacatcct cgttcgccgt
     1141 catcttgtag cccagcaggg agtgcacggt caggaagtcg accggggtgt cgatcttcgc
     1201 cgcgagcacg gacagcgcct tgtgtgtcgg ggcggcaacg gcgagcttgc gcactccgcc
     1261 ctgtttcagt tcgctgatca acttaccgac cagaaaggtc ttcccggtcc cggcatagcc
     1321 ggacagcacc gccatgccgg ccgtcgaatc cagactggcg aagttcatca gagagtcaaa
     1381 agcagcttgt tgatgtggcg tcagagtcat ttttttggaa ggttgacgaa gttgtcgaag
     1441 gttgtcacag ggcgtcaacc ttccaaaccc atgcgtagca agggtttggc gggaaggttg
     1501 tcgggttgac gcgcaaggtt cacggtttac ctctatagat attggctaag caaaaagcgt
     1561 caactcgtca accatcggcc gcaaagcctt gtgtcgactg gctttggagg gttgacggcg
     1621 agtgacaact ttcgacaact tcgtcagcct tccagccg
//
LOCUS       DAOQEW010000413         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_49414_length_1657_cov_7.807116, whole genome shotgun sequence.
ACCESSION   DAOQEW010000413 DAOQEW010000000
VERSION     DAOQEW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49414_length_1657_cov_7.807116"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..811
                     /locus_tag="PLS35_20655"
     CDS             266..811
                     /locus_tag="PLS35_20655"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNI21137.1"
                     /translation="MKLLSVQVGRPRTVRWHGKVVSTGIYKEPVEGRLPLQLFNLKGD
                     EQADLTVHGGREKAVYVYPSEHYPFWRKEYPDLPFHPGWFGENFTVEGMDERTVCIGD
                     QFRIGGALVVVTQPRMPCFKLGIRLGKPDAVKRFHASGRCGFYLGVLQEGEVGPGDLW
                     EQVARGDEQMTVIESYRLMTQ"
     gene            1103..1303
                     /locus_tag="PLS35_20660"
     CDS             1103..1303
                     /locus_tag="PLS35_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21138.1"
                     /translation="MSKVEAIEQQIEKLSSDELAAFRRWYVAFDAETWDRQFENDVKA
                     GRLDALAEKALRAHTSGQSRPL"
BASE COUNT          364 a          447 c          482 g          364 t
ORIGIN      
        1 cattgccaga ccaagtatcc gccgccgatt tcgaacagac ccgccagcac gaacaaggcg
       61 atcgattgtg tgatgagcat gggttctctc attgtttttc ttctggtaag aaatcgacgt
      121 ttcgttcgaa ttgacgacac gcgctccagg tgacgtaccc gagcccggcc ggttcgatgg
      181 cgaggcgagg gggcggtatg agatcatgac gacaacggac gtgcaagtcc aagggggcag
      241 cgccttacgt tcagaggagg cttccatgaa acttctctcc gtgcaagtcg gacgtccccg
      301 cactgtcaga tggcatggca aagttgtctc gaccgggatc tacaaagagc cggttgaggg
      361 ccgtctcccg ctgcagctct tcaatctcaa aggcgacgaa caggcggacc tgacggtaca
      421 cggcgggcga gaaaaagcgg tgtacgtcta tccgtcggag cattacccgt tctggcggaa
      481 agaatatccc gacctgccgt ttcacccggg atggttcggc gagaacttca ccgtcgaggg
      541 gatggacgaa cggacggtgt gcatcggcga tcaattccgg atcggcggcg ccctggtcgt
      601 ggtgacccag ccgcgcatgc cttgtttcaa actcggcatc cgactcggca agcccgatgc
      661 ggtgaagcgg ttccatgcga gcggtcgctg cggcttctat ctcggcgtgc tccaggaagg
      721 ggaggttggt ccaggcgatc tctgggaaca ggtcgcccgg ggtgatgaac agatgaccgt
      781 catcgaaagc taccggctga tgacccaata gcggcaagcc cagttcactt ttcatctctc
      841 aacttcccac aacaagcagg gcttagtttg ccccaaactg cgcgcgtcca acgagggaga
      901 gtctgcgacc gcgcgttgtg cgagcagtgg ggagatcatc aggccgccat ttctcttgcc
      961 ccatcggcct aaaccggggc tgggtgatcg tattggaagt tggacggcat ccttttctaa
     1021 tgctaggata cgaacattct gattgctgat ggacagcaga gagacagagg ttgtctctga
     1081 tcctggaaaa tgtaggtgat gtatgagcaa agtagaagcg attgagcagc agattgaaaa
     1141 actttcctcc gatgaacttg ccgcgtttcg ccgatggtat gtggcatttg atgcagagac
     1201 atgggatcgc cagttcgaaa atgatgtgaa ggccggaagg cttgatgccc tcgctgaaaa
     1261 agctcttcgt gcccatacct caggacagtc taggccgctt tgattcatca tacctcaccg
     1321 gaattttggg cctgttataa tgcccttcct gctacaatcc gtgagcaagc tgaccgcgcg
     1381 tttgaattat taaaggccaa ccctcgacat ccgtcgctgc atttcaagca agtaggccgg
     1441 ttttggtctg cacgtgcagg tcttcagtat cgagcggttg gagtggaagc gcctgacggc
     1501 atcgtctggt tctggatcgg cacacacgcg gagtacgaca aacttatcca gaagtaaggc
     1561 aggcagccaa ccggtcttta tcccgccgca aggttctttt caggttttcc ttcctacctc
     1621 cccacaccaa gaggggctcg gtttgcccca aactgcg
//
LOCUS       DAOQEW010000414         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_49554_length_1655_cov_5.809375, whole genome shotgun sequence.
ACCESSION   DAOQEW010000414 DAOQEW010000000
VERSION     DAOQEW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49554_length_1655_cov_5.809375"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLS35_20665"
     CDS             complement(<1..553)
                     /locus_tag="PLS35_20665"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNI21139.1"
                     /translation="MQIIMIPTSQIRVHDEYATLFPPLTSSERASLKTSIQEAGIHDP
                     LRVERQPTDAASVQPYVVLGGHHRLSIAQELNIPEVPCIIVTTMELKVAALFDNIHRR
                     QLPDTLRRQMQDEEQRYRARLRERLIPEILDVLPMLPPEVQLQLTQSSHDTQRDFLSK
                     WVAQVQRVGIQPQSEPQHTSPAPV"
     gene            552..737
                     /locus_tag="PLS35_20670"
     CDS             552..737
                     /locus_tag="PLS35_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21140.1"
                     /translation="MPGSLLLAVTSDDSVNVTSGQVVAATMKLPLELMTGDCGLSAKD
                     AISNLLSTGFWLGEVLG"
     gene            complement(1069..>1655)
                     /locus_tag="PLS35_20675"
     CDS             complement(1069..>1655)
                     /locus_tag="PLS35_20675"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNI21141.1"
                     /translation="HSIAKRYCNLDRSYEIEDLEQIGFLAVRKATEKWDFSRGNAQFQ
                     TFLYRYIQKAYQSEFDGRDKLVEITDKDDQYVKTLPYSTFIKEKRRLHSQGFKSRIFR
                     RLVPLTTHEPPVVPDASAESLAQTDVPGTDDVLGFTATDRFIEFSRKRARAIPDPTAL
                     DHDLLTTSEQSITGSSSGSGNHARQARKRRTYAN"
BASE COUNT          316 a          400 c          547 g          392 t
ORIGIN      
        1 ctaccggggc ggggctggtg tgctgtggct ccgactgggg ctgaatgccc actcgctgca
       61 cttgggccac ccacttggag aggaaatcgc gctgagtgtc gtggctggat tgtgtcagct
      121 ggagttgaac ttccggcggc agcatgggga gtacatccag aatctctgga atcaaccgtt
      181 ccctcagcct ggctcggtaa cgctgttcct catcctgcat ctgtcgacga agggtgtccg
      241 gcaattggcg tcgatggata ttgtcgaaca gcgcagcgac cttcagttcc atggtggtga
      301 cgatgatgca ggggacctcg gggatgttca gttcctgcgc gatgctcagg cgatgatggc
      361 ctccgagtac tacatagggc tggacggagg ctgcgtcggt aggctgtcgt tcgactcgca
      421 ggggatcatg gatcccggct tcttggatac tggttttcag gctcgctctc tctgacgacg
      481 tgagcggcgg aaagagcgtg gcgtactcgt catggactcg gatttgagat gtaggaatca
      541 tgatgatctg catgccgggc tccttgttgc tggctgtgac gagcgatgat tctgtaaatg
      601 tgacgtcggg acaggtcgtg gccgcgacga tgaagctccc gttggagttg atgactggtg
      661 attgtgggtt gtcggcgaag gatgccatta gcaatctgtt gagcacagga ttctggctcg
      721 gcgaggttct tggttagctg tcgtcggatc gcaaagacgc gatggatctc ggacaaatag
      781 cggagaggaa cgacatattg ccgacctcga tagcggcctt gagagggggt ggtaacgatg
      841 taccactggg ccggtgcatg ctggaggatg gcttgtaccg gactgggtag actctcccgt
      901 tccaacgtta accaacgata gagtttccaa tcactgcagg gaatgttgtg gccccggcag
      961 atgtcccgga acgcgctcat ggggagcacg ggctccgcgg gcatcggagt cggacccgcg
     1021 cgaagtccca cctgccggca gatggtttcg gctatgatcc gtgtcatatc agttggcata
     1081 ggtccgtcgt ttccgtgcct ggcgagcgtg gttgccgctg ccggaggaag atcccgtgat
     1141 cgactgttcc gacgtggtta gcaggtcgtg atcgagagca gtcgggtcgg ggatcgctct
     1201 ggcgcgtttg cgcgagaatt cgatgaaccg atcggtagcc gtgaaaccca agacgtcgtc
     1261 ggtgccgggc acatcggtct gtgccaaaga ttcagccgag gcgtcaggga cgacaggcgg
     1321 ttcgtgggtc gtcaaaggga caagccgccg gaagatacga cttttgaagc cttggctgtg
     1381 taagcgccgc ttctctttga tgaaagtgga atagggcagt gtcttgacgt attgatcgtc
     1441 cttgtcggtg atttcgacca gtttgtcccg tccatcgaat tcgctttggt aggctttctg
     1501 aatatagcga tagaggaagg tttggaattg ggcgtttcca cgggaaaagt cccatttctc
     1561 agtggccttc ctgacggcca gaaagccgat ctgctccaag tcttcaattt cataggagcg
     1621 atctaaattg cagtagcgct tcgcgatcga gtgga
//
LOCUS       DAOQEW010000415         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_50076_length_1646_cov_8.283470, whole genome shotgun sequence.
ACCESSION   DAOQEW010000415 DAOQEW010000000
VERSION     DAOQEW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50076_length_1646_cov_8.283470"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLS35_20680"
     CDS             <1..435
                     /locus_tag="PLS35_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNI21142.1"
                     /translation="AWFHAVIRGDVNYIRIGNRTNVQDLCMLHVTHDTHPLIIGDDVT
                     IGHHVVLHGCTVQNRVLVGMGAIIMDGAVIGEDSVVGAGALVTEGTIVPPKSLILGSP
                     AKVKRPVTEQELAWIRESAQNYIRYSRQYLTGPDKPRPGFWV"
     gene            complement(466..1164)
                     /locus_tag="PLS35_20685"
     CDS             complement(466..1164)
                     /locus_tag="PLS35_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21143.1"
                     /translation="MARKRMSVGPATRRAIKDIFSRLDYAQLGPIYCDEGGDEFWAAK
                     RGPCQRLGTKVATALKARLKPGGTSVYVGAGVAEIPPLVMEALELDRQVVACNLRRQE
                     VLLLNRACGELPIRFQHVDAGSVKQRVDHLWLVSVLNDPERFPELSVLSYGRANPVTF
                     NAKKFVTQQRTVARLVDRCLSRLTVPGLVTTTVEEAVWVAEWCHRRGIPYRIDERTYA
                     SPTVGDPICLMRIG"
     gene            complement(1393..>1646)
                     /locus_tag="PLS35_20690"
     CDS             complement(1393..>1646)
                     /locus_tag="PLS35_20690"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNI21144.1"
                     /translation="VMLTDVKGIRDANGRHLSTVSRKDVQRMVKRGTISEGMLPKVHA
                     CLDALAGGVGKAHIVDGRVPHAILLEIFTHKGIGTEIVS"
BASE COUNT          345 a          533 c          442 g          326 t
ORIGIN      
        1 gcctggttcc atgccgttat ccgcggggat gtgaactaca ttcgtatcgg gaaccggacc
       61 aacgtgcagg acctctgcat gttgcacgtc acacatgaca cccatcccct catcatcggc
      121 gatgatgtca ccatcgggca ccatgtggtg ctgcatggct gcaccgttca gaaccgggtg
      181 ctggtcggaa tgggcgccat catcatggac ggcgcggtga tcggggaaga ctccgtcgtc
      241 ggcgcaggag cgctcgtcac agaaggcacc atcgttccac cgaagagcct cattctggga
      301 tcaccggcca aggtcaagcg tccggtgacc gagcaggaac tcgcatggat acgggaatcg
      361 gctcagaatt atattcgata ttcccgtcag tacctcaccg gaccggataa acctcgcccg
      421 gggttttggg tctaacgacg ccaccacaat cagccgccac gctccttatc cgatccgcat
      481 gaggcagatg ggatcaccga ccgtgggtga cgcatacgtg cgctcgtcaa tccggtaggg
      541 aatgcctcgt cgatggcacc actcggcaac ccacaccgcc tcttcaacgg tggtggtcac
      601 taaacccggc acagttaatc ggctcaggca acgatcgaca agtcgggcaa ccgtgcgttg
      661 ctgcgtgaca aattttttcg cattgaaggt cacgggattc gcccgcccgt aggacagcac
      721 cgataattcc ggaaagcgtt caggatcatt cagcacactg accagccaga gatgatccac
      781 ccgctgcttg accgaacccg catccacatg ctgaaaccgg atggggagct cgccgcaggc
      841 acgattcaga agaagcacct cctgccgccg taagttacag gccaccacct gccgatccag
      901 ctccagcgct tccatcacca gcggcggaat ctccgccacg cccgcgccga catacacact
      961 cgtgccgccc ggcttgagcc gggccttcaa cgccgtggct actttcgtcc cgagacgctg
     1021 acagggccct cgcttcgcgg cccaaaactc gtcgccgcct tcatcgcaat aaatcgggcc
     1081 tagctgcgca tagtcgagtc gcgaaaatat atctttgatc gcccgcctgg tggcagggcc
     1141 tactgacata cgtttacgcg ccatgtttct ccgtccagac tcagtccttg atgacgtgct
     1201 tcgctctgag atgtttatga cactggcata cggctcgtgc gcatagacga gggagacggg
     1261 ttagcatacc ggcgactgaa ccattcatct cagcgccgac gcagtcgttc cctattggcc
     1321 ccaatacgcc cggccccaga ggtccttcac accgctgttg gttgaggtat acccgcgtgg
     1381 ttggttcact gcctacgaaa cgatctcagt gccgatgcct ttgtgagtaa atatttccag
     1441 cagaatggca tggggcaccc gcccatcgac gatatgcgct tttcccaccc caccggccaa
     1501 ggcatccaga caggcatgca ccttgggcaa catcccttcg ctgatggtgc cgcgcttcac
     1561 catgcgctgc acgtctttgc gggagaccgt cgagagatgg cggccattgg catcccggat
     1621 gcccttcaca tcggtcaaca tcacga
//
LOCUS       DAOQEW010000416         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_50439_length_1640_cov_5.340063, whole genome shotgun sequence.
ACCESSION   DAOQEW010000416 DAOQEW010000000
VERSION     DAOQEW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50439_length_1640_cov_5.340063"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1410
                     /locus_tag="PLS35_20695"
     CDS             283..1410
                     /locus_tag="PLS35_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21145.1"
                     /translation="MAHSYTPGLTVTERTTVRRRRILPLPGAVLVQVGDAVRADTAVA
                     RAELPGKVVPLNLANQLGIAPDEINDYLVKKEKDSVQKDDVLAENKPFIKWFKTEIRS
                     PITGKVESVSTITGQILLREPPRVLELRGYIDGVVAEVHPEQGVTVESTCSLVQGIFG
                     IGGETSGELVMGVKAPDQPLLPEQLNPSMKGKVVVGGAFLSAATMTRAKELGIVGLVV
                     GGIHDKDLRALLGYDLGVAITGTEQVGFTLILTEGFGTIPMAPKTFALLSAHAGQKAS
                     ISGATQIRAGVIRPEIIIPHSGPAAVTKLPKAEREGIQLGDPVRIIRDPLFGKIGAVS
                     GLPSELRAIPTESEVRVLEVRFPDGTTTVVPRANIEVIEGA"
     gene            1416..>1640
                     /locus_tag="PLS35_20700"
     CDS             1416..>1640
                     /locus_tag="PLS35_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21146.1"
                     /translation="MLHRAIILIVMICLPALLPSLVPAQTMPQQLRVIDTPSDGGGSL
                     TVLWASSATDSATAKYQILVHEGGLPQDPTA"
BASE COUNT          343 a          509 c          495 g          293 t
ORIGIN      
        1 cagttgccca cggagcaggc cgcgcgagtc gccctgctga cacgctggta tcaggccgtc
       61 gggttgtacc cgacggggag ctcatagtga gcgcgtatgg ctcatgacga gagacaacat
      121 gcggccaagc ccgcaatgga catcattggc tttaagaggt tgctcaacac gtcttgcagc
      181 aaggccgcaa ggcgccagag ccccgagtcg tactcttgat gtacggcgag gggaatgagc
      241 aactgagaac gacactgcaa ggcgtgttca gcaaccgacc atatggcaca tagttacaca
      301 ccaggcctga ccgtcactga acggacgacc gtccgccgcc ggcgtattct ccctttgccg
      361 ggtgccgtct tggtccaagt cggcgatgct gtgcgagccg acaccgcggt ggcccgcgct
      421 gagttaccgg gaaaggtggt gccgctcaac cttgcgaatc agctcggcat cgcaccggat
      481 gagatcaacg actatctggt caaaaaggaa aaggactccg tccagaaaga cgacgtcctc
      541 gccgagaaca aacccttcat caaatggttt aagacggaaa ttcgatcccc catcacgggc
      601 aaggtggaat ccgtgtccac gatcacggga caaatcctgt tacgcgaacc gccgcgagtg
      661 ctcgagttgc gcgggtacat cgacggggtc gtggctgagg ttcatcccga acagggtgtg
      721 acggtggaaa gcacctgcag cctcgtgcaa ggcatcttcg gcatcggggg agaaaccagt
      781 ggcgaactcg tcatgggtgt gaaggcgccg gatcagccgc tcctgccgga acagctcaac
      841 ccatccatga agggcaaggt cgtcgtcgga ggcgcgtttc tctcggcggc cacgatgacg
      901 cgcgcgaaag aactcgggat cgtcggcctg gtcgtcggcg gcatccatga caaggattta
      961 cgcgcgttgc tcggatatga tctgggcgtc gcgatcaccg gcacggagca ggtcggcttc
     1021 acgctgatcc tgacggaagg gttcggcacg attcccatgg caccgaagac gtttgcgctg
     1081 ctctccgccc atgcgggaca aaaggcgtcg atctcgggag ctacgcagat ccgcgccggc
     1141 gtgatccgcc cggaaatcat cattccacac agcggcccgg ctgcggtcac gaagctgccc
     1201 aaggccgaac gggaaggcat tcaactcggc gatccggtcc ggattatccg cgatccgctg
     1261 ttcggcaaga tcggcgcggt ctccgggctg ccgtctgaat tgcgcgccat tccgaccgaa
     1321 agcgaagtcc gcgtgctcga agtccgattt ccggacggca cgacaaccgt ggtcccccgc
     1381 gctaatatcg aagtgatcga aggagcctga accgcgtgct gcatcgcgcc attattctca
     1441 ttgtgatgat ctgcctcccc gcgctgctcc cgtcgctcgt cccggcgcag accatgccgc
     1501 aacagcttcg cgtgatcgat acccccagcg acggaggcgg aagtctcaca gtcctctggg
     1561 cttcttccgc aaccgacagt gccaccgcca aatatcaaat cctggtccat gaaggtggtc
     1621 ttcctcaaga tcccacggcc
//
LOCUS       DAOQEW010000417         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_51108_length_1628_cov_6.078830, whole genome shotgun sequence.
ACCESSION   DAOQEW010000417 DAOQEW010000000
VERSION     DAOQEW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51108_length_1628_cov_6.078830"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..>1628
                     /locus_tag="PLS35_20705"
     CDS             298..>1628
                     /locus_tag="PLS35_20705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNI21147.1"
                     /translation="MTKSAAPGLLPSNGGFALNYPDRPWYALSACILLATLLIDLYTP
                     AGIPASLLYLLAVAAVGLVSGSRMHWIVAAISTGLTVVGLFGTSPESIGWIEVTNRAL
                     SIGAAWLTAWIVSQWQKCARSRAHDVSIRLRSLLTHSQTIIFVKDLQGRYLEVSEQFL
                     TLVGVPAERVIGKTDHDLFPRDIADSYRQHDCEVVHAGGVMSFEEDALLNHDPRFYVV
                     RKFPLRDEAGRIIAVGGVATDMTARLQAEVARHDAQERLDLVVAATKTGIWDWDLQAN
                     TMYYSPRWKSSLGYEEQEISNSPSEWESRLHPDDKKRALALVDDYLSGRIASYELEHR
                     LRHKDGTYRWIHTCAVFQRDEQGRPRRMTGSHVDITERKRAEEALSRSESTLRGLFES
                     GVMLMGVVELHDGDILHLSDNRCAAGFFGTTPELMQGKWASKLGAPPDTIA"
BASE COUNT          313 a          428 c          500 g          387 t
ORIGIN      
        1 gttgagtgtt tgctccgtgg tttgacgtgt cccgttgggg acaccacact ttctcatcga
       61 tatttgttgc cagacttttt ccgggaaggc cccttttcgg ataaagtagg ccgcacctag
      121 acagcaatac gaagtggttg acggcatcat ggaggatggt gtctgtcgtg ggagaggatc
      181 tctcgtagat cgataagtgg ccgagtcgcg ccacggggat ctcgaccgcg aagctgcatc
      241 gcttgcccgc ctcatgatcc ggcgtcgttg cgagagcgtg acgtacggag tctttctgtg
      301 accaaatcag ctgcccctgg ccttctgcca tctaacggtg ggttcgcctt gaactatcct
      361 gacaggcctt ggtatgccct cagtgcgtgt atccttctcg ctacgctcct gattgacctc
      421 tataccccag caggcattcc agccagcctg ctgtatctgc tcgccgtcgc cgcggtcggg
      481 ctggtgtcag gctcgcggat gcattggatt gtggcggcca tttctaccgg tctcacagtg
      541 gtcgggctgt ttgggacttc tcccgagagt atcggctgga ttgaggtcac caatcgagcc
      601 ctttcaattg gtgctgcctg gctgacggcg tggattgtct cccagtggca gaaatgtgct
      661 cgcagccgtg ctcacgatgt gtctattcga ttgcgctctc ttctcactca tagccaaacc
      721 atcatctttg tcaaagatct tcagggccgc tatctcgaag tcagcgagca attcctcacg
      781 ttggtcggcg ttccggccga gcgggtgatt ggaaagacgg atcatgacct gtttccccga
      841 gatatcgcag acagttaccg acaacatgat tgtgaggtcg tgcatgccgg cggagtgatg
      901 agcttcgagg aagatgcgtt gctgaatcac gatcctcgct tctatgtggt gcgcaagttt
      961 ccccttcgcg acgaagcggg gcgaatcatc gcggtcggtg gtgtggccac ggacatgaca
     1021 gctcgcttgc aggctgaagt ggcgcggcat gacgctcagg aacggttgga tttagtcgta
     1081 gccgcgacga agaccggaat ttgggactgg gacctgcagg caaacacgat gtattactcc
     1141 ccccgttgga agtcgagtct gggatatgag gagcaagaaa tctccaattc gccatctgaa
     1201 tgggaatccc ggctgcatcc ggatgacaag aagcgtgcgt tagccttggt ggacgactac
     1261 ctttcgggcc ggatcgcgtc gtatgaactc gagcatcggt tacgtcataa agacggaacg
     1321 tatcgatgga tccacacctg cgccgtgttt caacgagatg agcaggggcg cccccggcgc
     1381 atgacgggtt cgcatgtgga cattacggag cgaaagcgag cggaagaggc gctgtctcga
     1441 agtgagtcca cgcttcgagg tttgttcgag agcggagtga tgttgatggg ggtggtggag
     1501 ttacacgacg gcgatattct gcacctttca gacaatcgtt gcgcggccgg cttttttggc
     1561 accactccgg agttgatgca gggaaagtgg gcctcgaagc tcggggcgcc gccggacacc
     1621 atcgcatt
//
LOCUS       DAOQEW010000418         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_51795_length_1617_cov_6.805378, whole genome shotgun sequence.
ACCESSION   DAOQEW010000418 DAOQEW010000000
VERSION     DAOQEW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51795_length_1617_cov_6.805378"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..977)
                     /locus_tag="PLS35_20710"
     CDS             complement(321..977)
                     /locus_tag="PLS35_20710"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNI21148.1"
                     /translation="MGHRGAAGHAPENTLAAIWKARSFHADFVEIDLRETSDGHLVLL
                     HDDTIDRTTNKSGNLAEMSLEQVQRLDAGNWQRIPTLEEALDISGKAIGVILELKVEG
                     IGNEACAIVKRVGFSNSLIYASFLIPELHRVRQADPAAQIMVLLHRHLPLDPVADVVA
                     LNASHVGLHYSTVTPALLQTYHDLGRQVFVYTVNDWTEIRRMRELGVDGIVSDFPDRI
                     "
     gene            1256..>1617
                     /locus_tag="PLS35_20715"
     CDS             1256..>1617
                     /locus_tag="PLS35_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNI21149.1"
                     /translation="MLVQEVMSTGVVTAKRNESVRSVVTKMLSRHCGAIPVVEDGDVL
                     IGMVTLRDVLIPLYPNYGEYIHDNVHSRDFVEMEEGYADVLSQRVEEVMSLNPLTVSP
                     RTPVLEAASYMGVKNFRR"
BASE COUNT          313 a          442 c          496 g          366 t
ORIGIN      
        1 gcgccaacca cgggcgacct cccaccgcag tgtagggtcg cccgtggcat tttaagccgg
       61 attgcttttg acagtcttgc ccctctcccc ttccttcggc ctcccgcctg accagatctc
      121 attctttgac ggtgtttctc tgcctccgcc ccatcttgta tgcctggcca agtcggatca
      181 tgctccatcg gcgttttgcc caaaaggtgt ttgcgcatcc tcgcgttcga ggtgccgtct
      241 cgtgaggtcc gctcggtgtt gatcggcagc aaagcagagt gcgtgctctg cctgattgtg
      301 agagctggcc ggccgtgagg tcagatgcga tccggaaagt cggagacaat gccgtctacg
      361 cccaattccc gcatgcgccg gatctccgtc caatcgttca cggtatagac gaatacttgt
      421 cggcccaagt cgtggtaggt ctgaagcagt gcgggcgtga ccgtcgaata gtgcaatccg
      481 acgtgggagg cgttgagggc caccacatcg gccacagggt cgagcgggag gtggcgatgt
      541 agtaacacca tgatctgtgc ggcgggatcg gcttgtcgca cgcgatggag ttcaggaatc
      601 aagaacgagg catagatgag cgaattggaa aagcccacac gtttcacaat cgcgcaggcc
      661 tcgttgccga tgccctccac cttcagttcg aggatcactc cgatggcctt tccggagata
      721 tccagcgctt cttctaatgt ggggatacgt tgccaattgc ccgcatccag ccgttgcacc
      781 tgctccaacg acatttcggc gaggttgccg gatttgttgg tggtgcgatc aatggtgtcg
      841 tcatgcagca atacgagatg accgtcgctg gtttcgcgca ggtcgatctc cacgaaatct
      901 gcgtgaaagg atcgagcttt ccaaatagcg gcgagagtat tttcgggagc gtggccggcg
      961 gctccgcgat gaccgatgcg caggacggaa tcgatagcga cctcgcgtga gaaggtctga
     1021 agggtgctcc ccgctcatat gtggcgacgg cgagaggggg tttcttttct aaccgcactt
     1081 ccctaccata ttttgcatat ctctgcaaag gacagttgac gcggacggga tgtgtggcgg
     1141 tgtcgaggaa ccgtcctcgt caggagcgaa gtggcattga gctgcccaag acggcgaccg
     1201 aatcgtgctt tcggtgatgg actcacaggt caccctgctg aaaaaggagg ttgccatgct
     1261 cgttcaggaa gtcatgtcca ccggtgtggt gacggccaag cgcaatgaat ccgtccgttc
     1321 cgtggtgacg aagatgctca gccgccattg cggcgccatt ccagtcgtgg aggacggcga
     1381 cgtgttgatc ggcatggtga cgttgcgcga tgtgttgatt cccctctatc ccaactacgg
     1441 ggagtacatt cacgacaatg tgcacagtcg tgatttcgtg gaaatggagg aagggtatgc
     1501 cgatgtcctc tcgcagcgtg tcgaagaggt gatgagcttg aatccgctga cggtgtctcc
     1561 acgcacgccg gtgctggagg ccgcgtccta tatgggcgtc aaaaactttc gccggat
//
LOCUS       DAOQEW010000419         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_54981_length_1568_cov_8.544613, whole genome shotgun sequence.
ACCESSION   DAOQEW010000419 DAOQEW010000000
VERSION     DAOQEW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54981_length_1568_cov_8.544613"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLS35_20720"
     CDS             complement(<1..227)
                     /locus_tag="PLS35_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNI21150.1"
                     /translation="MKIEVRGLTKKFGSGTAVGNVSFVVHEGELLGLLGPSGSGKTTV
                     LRLIAGLEVPTSGDIFIDGKRVNDLTVQERN"
     gene            complement(232..1041)
                     /locus_tag="PLS35_20725"
     CDS             complement(232..1041)
                     /locus_tag="PLS35_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HNI21151.1"
                     /translation="MRWLLIGIVWLYFLVLLVGPILYMASQSFSEGLAAFWTEISRPE
                     AVHGFTLTAEITLIVLVLNLIFGTLTALVLARQQFWGRSLVSGVIDLPFAVSPVIAGF
                     MLILLFGPDTMLGALFGQAHIKILFALPAMILATLFVTFPFMVRELTPLLQTLGTEEE
                     EAARTLGAGEWQVFLKVTLPALRWGLVYGATLTVARAIGEFGAVLVVSGNILLLTQTA
                     TLHIYQSYVDFNYVAANAVALTLLAVSFAILTVLEIAKARAETTVAEAGAQ"
     gene            complement(1090..>1568)
                     /locus_tag="PLS35_20730"
     CDS             complement(1090..>1568)
                     /locus_tag="PLS35_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNI21152.1"
                     /translation="PGIILAMLFVTMPFTIRSLQPVLMGLERDQEEAAFTLGASPWTT
                     FWKVTVPSVLPGLLTGVFLTFVRALGEFGSIVIVAGNIPMKTQVASVYVYGEIESYNP
                     QAATSVSVLILLISFVVLLLLERLTRRPGERLPKLFRWANRHDQPGRDAAAATAAH"
BASE COUNT          347 a          488 c          462 g          271 t
ORIGIN      
        1 atgttgcgtt cctgcacggt gaggtcgttg acgcgcttcc catcgatgaa gatgtcgccg
       61 gaggttggga cttcgaggcc tgcaatcaac cgcagcaccg tggtcttgcc gctgccgctc
      121 gggccgagca gtcccagcag ttcaccttcg tgcaccacga acgaaacatt gccgaccgcc
      181 gttccggaac cgaatttttt ggtgagcccg cggacttcta ttttcaccgg cttactgcgc
      241 tcctgcttct gcgacggtcg tttccgctct ggccttggcg atctccagca ccgtgaggat
      301 cgcgaaggag acggcgagca gggtcaaggc cacggcattg gccgccacgt aattgaaatc
      361 gacataactt tgatagatat gcaaggtggc agtctgggtc agcaacagga tattgccgga
      421 cacgaccagc acggctccga attcgccgat ggcgcgcgcc acggtgagcg tggcgccata
      481 aaccagaccc cagcgcaagg cggggagcgt caccttgaga aagacttgcc actcgcccgc
      541 ccccaatgtc cgtgcggcct cttcttcttc cgtccccagc gtctgcagca gaggcgtgag
      601 ttctcgcacc atgaagggaa aggtgacgaa cagggtggcg agaatcatgg cgggcagcgc
      661 gaagagtatc ttgatatgcg cctggccgaa caacgcgccc agcatggtgt ccggtccgaa
      721 cagcaggatc agcatgaatc ccgcgatcac gggcgagacg gcaaaaggca aatcgatcac
      781 gccgctgacc aggctgcggc cccagaactg ttggcgcgcc aacacgagcg ccgtgagggt
      841 tccgaaaatc aggttgagca cgaggacgat cagcgtgatt tccgcggtga gggtgaagcc
      901 atgcaccgct tccggccggg agatctccgt ccagaacgcc gccagcccct cgctgaaact
      961 ttggctcgcc atgtacagaa tcgggccgac caggagcacc agaaaataga gccagacgat
     1021 tccgatcaac aaccaacgca tgcccgcact cccattacac gtcacacaat ctcaaacccg
     1081 gctggctttt cagtgcgccg ccgtcgccgc tgccgcatca cggccgggct gatcgtgccg
     1141 gttcgcccag cgaaacagtt tgggcaaccg ttcaccggga cgccgggtca accgttccaa
     1201 gagcagcagc accacaaacg aaatgagcag aatcagcacc gagacggagg tcgcggcctg
     1261 ggggttgtag ctttcgattt ctccgtagac gtagaccgac gccacctgag tcttcatcgg
     1321 aatattgccc gccacgatga cgatggagcc gaattcgccc aaagcccgta cgaaggtcag
     1381 aaacactccg gtcagcagcc cgggaaggac cgacgggacc gtcaccttcc aaaaggtcgt
     1441 ccaggggctc gctccgaggg tgaatgccgc ttcttcctga tcgcgttcca gtcccatcaa
     1501 caccggctga agggagcgaa tcgtaaacgg catggtgaca aacagcatgg ccagaatgat
     1561 gccgggct
//
LOCUS       DAOQEW010000420         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_55616_length_1559_cov_3.642952, whole genome shotgun sequence.
ACCESSION   DAOQEW010000420 DAOQEW010000000
VERSION     DAOQEW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55616_length_1559_cov_3.642952"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLS35_20735"
     CDS             <1..412
                     /locus_tag="PLS35_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21153.1"
                     /translation="ILAPALAKALSQASPSERVYYHLSHATEHGEEETSTGWLSIQDT
                     TLHLALWEAHDRHGPGPDISKYDRQMPNVPERSPAFDATFEPEEYLVKVRSGGGLFAP
                     DQEEELLIRYREALAAMPAQPGLDRESKPMPERH"
     gene            438..725
                     /locus_tag="PLS35_20740"
     CDS             438..725
                     /locus_tag="PLS35_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21154.1"
                     /translation="MAILVVGGDSVNAITERAYAGGHGCVEHWSGRKTRDPVKAISRK
                     TPAVVVVLDQVNHTLARKVRAEAIRRGLPVFFQKRGRQPPLDVTQWLVRIA"
     gene            772..>1559
                     /locus_tag="PLS35_20745"
     CDS             772..>1559
                     /locus_tag="PLS35_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNI21155.1"
                     /translation="MIFRTLNRLSILLLFLVPLSTPAHAETREVLLAAYSVPKEVYEK
                     RLIPAFVQKWKQRTGETIHIRSSFAASGAQARAIAGGLDADIAALSLESDLQQLVQAG
                     LVTHDWKQGPQHGIVTTSLVALGVRKGNPKAIHGWEDLARSGVEVLYPSPKTSGGAMW
                     DIIAIYGAGVQLGTRRGLSEAALHDSAGDLVARIQRNVKVMDKSGRESVTTFERGVGD
                     VIVTYENELLPRIAQGRPYELVYPDETVVVENPIALVDRHADRH"
BASE COUNT          346 a          456 c          469 g          288 t
ORIGIN      
        1 gatcctggcg ccggcgctgg cgaaagccct gtcacaagcc tcaccctctg aacgcgtgta
       61 ttaccatctg agccatgcga ccgaacacgg cgaagaggaa accagcacgg ggtggctctc
      121 gatacaagac acgacgctgc atctggcgtt atgggaggct cacgaccggc acgggccggg
      181 gcccgatatc agtaagtatg accgtcagat gccgaatgtg ccggaacggt ctccggcctt
      241 cgacgccacg ttcgagccgg aggaatatct ggtgaaggtt cgatcaggag ggggtctctt
      301 cgccccggat caagaggaag aattgctcat ccggtaccgg gaggcgttgg ctgccatgcc
      361 cgcgcaaccg ggattggatc gagagagtaa accgatgccg gaacggcact gagcaatcga
      421 gaaagaaggg agcatccatg gcaatcctgg tcgtcggcgg tgattcggtc aatgccatca
      481 cagaacgggc ctacgccggc ggccacggat gcgtcgaaca ttggagcggg agaaagacgc
      541 gtgacccggt gaaagccatc tccaggaaaa cgccagccgt tgtggtggtg ctcgatcaag
      601 tcaaccacac attggcccgg aaggtgcgcg ccgaagccat ccggcgaggc ctgcctgtct
      661 tctttcagaa acggggccgc caaccaccgc tggacgtgac gcagtggctc gtcagaatag
      721 cgtaacgatt gggtcatttt taccggacag atccttggga gtcttgccat catgatattc
      781 cgcactctca atcggcttag tatcctcctg cttttcctcg tcccgctttc cacgccggcc
      841 cacgcagaga cacgagaagt cctcctcgcg gcttacagcg ttccgaagga agtgtacgaa
      901 aagcgcctca tcccggcgtt tgtgcagaaa tggaaacaac gtaccggaga aacgatccac
      961 attcgaagct ctttcgccgc atctggcgca caggcccgtg ccattgcagg cggtctcgat
     1021 gcagacatcg ccgcactgtc gctggaaagc gatcttcaac agttagtgca agccggtctc
     1081 gtcacccacg attggaaaca ggggccacag cacgggatcg ttaccacctc acttgtcgca
     1141 ctcggcgtcc gcaaagggaa tccgaaagcg atacacggtt gggaagacct ggcgcgatcc
     1201 ggcgttgagg tgctctatcc gagtccgaaa acctccggcg gcgccatgtg ggatatcatt
     1261 gcgatctatg gagccggggt tcagctcggc accaggcggg gattgtcgga agcggcttta
     1321 cacgactccg cgggagacct cgtcgcccgg atccaacgta acgtcaaagt catggataaa
     1381 agcggtcggg agtccgtgac gaccttcgag cgtggtgtcg gcgatgtgat tgtcacgtac
     1441 gaaaacgaat tactcccgcg aatcgcccaa ggccgtccgt atgagcttgt gtatcccgat
     1501 gaaaccgtcg tggtggagaa tcccatcgct ctggtggacc gccatgccga ccgtcacca
//
LOCUS       DAOQEW010000421         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_56670_length_1544_cov_8.977166, whole genome shotgun sequence.
ACCESSION   DAOQEW010000421 DAOQEW010000000
VERSION     DAOQEW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56670_length_1544_cov_8.977166"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1506)
                     /locus_tag="PLS35_20750"
     CDS             complement(37..1506)
                     /locus_tag="PLS35_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21156.1"
                     /translation="MRLPWDATGDVTEAALRRIQIREAIKAHFDKEQALFQQGVKVLT
                     LYFIDEVVKYRDYSAPDEKGDYARIFEEEYRLYLNEMLDLDETPYIKYLKGISADKTH
                     SGYFSIDKKSKRLADPTVAARGENAGLSDDVDAYDLILKDKERLLSFAEPVRFIFSHS
                     ALREGWDNPNVFVICALKHSDNTISRRQEVGRGLRLSVNQSGDRMDHPATVHDVNVLT
                     VVASESYKDFVAALQKDISESLSARPRMADKAFFTGKVVATPTGPVVVTEDQATDIEF
                     YLIQNGYVDKKRNVTEKYHQAKKDGALVELPEELAPLGAQVFALIDSVFSESQLPEIG
                     DDRKPKKNPLNANFEKQEFKELWNRVNRKAAYSVNFDSDKLVQKAVKELDASLRVTPL
                     QYTIQHGEQAAAVTYDEIKSGNAFELKVTKTETSRASIHSAVKYDLIGKLAEGTQLTR
                     RTVTDILKGLNAAVFAQFKTNPESFIAEAIRLINQGKRI"
BASE COUNT          286 a          441 c          428 g          389 t
ORIGIN      
        1 gggctatctg atcaaggtgt tgaacgggca gatgatttag atgcgtttgc cctggttgat
       61 caggcgaatc gcctcggcaa tgaagctctc cgggttggtt ttgaactgcg cgaatacagc
      121 ggcattgagc cccttgagga tatcggtcac cgtgcgccgt gtcagctggg tgccttcggc
      181 aagcttgccg atcaggtcgt acttgaccgc agaatggatt gaggcgcgac tggtttccgt
      241 cttcgtgacc ttcaactcga acgcattgcc gctcttgatt tcgtcgtagg tcacggcggc
      301 tgcctgttcg ccgtgttgga ttgtgtattg cagcggagtg acgcgcaggc tggcatccaa
      361 ctctttgacc gccttctgca ccagtttgtc ggaatcgaag ttgacactgt atgccgcctt
      421 tcggttgacc cgattccaga gttccttgaa ctcctgcttt tcgaagtttg cgtttagcgg
      481 attcttcttg ggcttgcggt cgtcgccaat ttcgggcaac tggctctcgc tgaaaacgct
      541 atcgataagc gcgaatacct gcgcaccaag tggcgcgagt tcttcgggaa gctctaccaa
      601 tgctccgtct ttcttcgcct gatgatattt ctcagtgaca ttgcgcttct tatcaacgta
      661 gccgttctgg atcaggtaga actcgatatc tgtagcctga tcttcagtga caacgactgg
      721 cccagttggt gtcgccacca ctttgccggt gaaaaatgct ttgtccgcca tgcgcggacg
      781 cgccgacagc gactcgctga tgtccttctg cagcgcagcg acaaagtcct tgtagctctc
      841 gctggccacc acagtaagca cgttcacatc gtgtaccgtg gccgggtgat ccatcctgtc
      901 accgctttgg ttgacggaca gccgcaagcc acgtcccact tcctggcggc gggatatcgt
      961 gttgtcactg tgcttgagag cgcagatcac gaacacgttc gggttgtccc agccttcacg
     1021 cagggccgag tgggagaaga tgaagcgcac cggctcagcg aacgacagca gccgctcctt
     1081 gtccttcagg atcaggtcgt aggcgtccac gtcgtcggaa agccccgcgt tctcgccacg
     1141 cgccgcgact gtggggtcgg ccagccgttt gctcttcttg tcgatggaaa aataaccgct
     1201 gtgagtcttg tcggcagaaa tgccttttag gtacttgatg tacggcgtct catccagatc
     1261 cagcatctcg ttgagataaa gccggtactc ctcctcgaag atgcgggcgt aatcgccttt
     1321 ctcgtccggt gcgctgtagt cgcggtactt aaccacttcg tcgatgaagt agagcgtgag
     1381 caccttcacg ccctgttgga acagtgcttg ttccttgtcg aaatgcgcct tgatggcctc
     1441 gcggatctgg atgcgacgca gtgcggcctc ggtcacatcc ccagtcgcat cccagggcaa
     1501 acgcatcttc ccatcctatg aaaacgccat acatgacctg cact
//
LOCUS       DAOQEW010000422         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_57747_length_1530_cov_6.714576, whole genome shotgun sequence.
ACCESSION   DAOQEW010000422 DAOQEW010000000
VERSION     DAOQEW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57747_length_1530_cov_6.714576"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLS35_20755"
     CDS             complement(<1..293)
                     /locus_tag="PLS35_20755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250600.1"
                     /note="catalyzes the formation of 4-phospho-L-aspartate
                     from L-aspartate and ATP, in Bacillus, lysine sensitive;
                     regulated by response to starvation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNI21157.1"
                     /translation="MALIVQKYGGTSVGNIERIHRVAERVERAQKDGHQVVVVLSAMS
                     GETDRLLKLAHEATHSPDERELDMLLSTGERVTIALLAMELRGRGVNARSFTGR"
     gene            complement(299..>1530)
                     /gene="apgM"
                     /locus_tag="PLS35_20760"
     CDS             complement(299..>1530)
                     /gene="apgM"
                     /locus_tag="PLS35_20760"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNI21158.1"
                     /translation="ADGMADLACAELGGSTPLQAAKTPFLDQIAQRGEVGLLSLPSDA
                     GRTGSDVTGVAVLGYDPKKYYPGPGPLEAAGLGVTVGEQDVVFRCTMVTVRGESASGG
                     TDRAADIKKLGPHVVMEDATAGLIETEQARELVEAVNEQLGSESIQFYPGSGHRHLMV
                     WVGGKSRATCVDPQQLVGQSIAEALPAGDGADVLRKIMDASFFILRDHPVNEEREEDG
                     LKPANCLWLWGQGRAPLWPPLPERYQVSGVVVSASDVHRGVGLCAGLDAAELPAESGD
                     ESETFAGCVKAVVQELAKKDFVYLHAGLSDDVLHATNVHDKVQAIERFDAQVIGPILS
                     ALAKYPAYRLLVVCDPGLAKGQGSDVPPILYALCDGATQPPAGSSTRFHEQDKTIAGS
                     APRDATKLMMRLFPRGA"
BASE COUNT          316 a          518 c          398 g          298 t
ORIGIN      
        1 cggcctgtga acgatcgggc gttcacgcct cgcccccgaa gctccatggc caacaaggcg
       61 atagtcaccc gctctcctgt cgacagcagc atgtccaatt cacgctcatc cggggaatgg
      121 gtcgcttcat gcgccagctt aagcaatcga tccgtttcgc cgctcatggc cgacaacacc
      181 acgaccacct gatgcccgtc tttttgcgcg cgctcgaccc gttcggcgac gcgatgaatc
      241 cgctctatgt ttccgaccga cgtgccgccg tatttctgaa cgatcagtgc cacaaccctt
      301 aggctcctcg aggaaataat cgcatcatga gtttcgtcgc atcgcgagga gcggatccgg
      361 cgatggtctt gtcctgttca tgaaaacggg tggacgagcc agccggtggt tgggtggcac
      421 cgtcacacaa cgcatacaaa atcggcggaa cgtccgagcc ttgccccttt gccagaccgg
      481 gatcgcacac cacgagcaga cggtacgccg gatacttcgc taacgccgaa agaatcggtc
      541 cgatcacctg ggcgtcgaaa cgttcaatcg cctgtacctt gtcatgcaca ttggtcgcgt
      601 gcagcacatc atccgacaga ccggcatgga gatagacaaa atctttcttc gccaattcct
      661 ggacgactgc ctttacacag ccagcaaacg tttctgattc atcgccactc tcggccggca
      721 gctcggccgc atccaatccg gcacagaggc caacgccgcg gtgcacatca ctggctgata
      781 ccaccactcc ggagacctga taccgttccg gcagcggcgg ccacaacgga gcgcgcccct
      841 gcccccataa ccacaaacaa ttggccggct tcagcccgtc ttcttcacgc tcctcattga
      901 ccgggtgatc acgcaggata aaaaaggagg catccatgat tttccgcagc acgtctgcgc
      961 catcaccggc cggcagagcc tcggcgatcg attgtccgac gagctgctga ggatcgacgc
     1021 aggtcgcacg ggatttccct ccgacccata ccattaaatg ccgatgacct gagccggggt
     1081 agaattggat actttccgat cccaattgtt cgttgacagc ctcaactaac tcgcgagcct
     1141 gctccgtctc gatgagcccg gccgtcgcat cttccatgac cacatgcggt cccagcttct
     1201 tgatgtcagc cgcacggtct gttccgccgg aggcggactc gcctcgcacc gtgaccatcg
     1261 tgcaacggaa caccacgtcc tgctcaccca ccgtcactcc gagaccggca gcctccaggg
     1321 gacctggccc cggataatat tttttgggat cataccccaa caccgcgaca ccggtgacat
     1381 cgctgcccgt cctgcctgca tcggaaggaa ggctcaacag ccccacttct ccgcgttggg
     1441 caatttggtc aagaaagggg gtcttggcgg cctggagagg cgtgctccca ccgagttccg
     1501 cacaggcaag gtctgccatc ccgtcggcat
//
LOCUS       DAOQEW010000423         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_57756_length_1530_cov_4.644746, whole genome shotgun sequence.
ACCESSION   DAOQEW010000423 DAOQEW010000000
VERSION     DAOQEW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57756_length_1530_cov_4.644746"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLS35_20765"
     CDS             complement(<1..241)
                     /locus_tag="PLS35_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21159.1"
                     /translation="MTTLFLAWQAPVSRNWFPVGRLDADAGRERFNFRYTQGVIAAKE
                     HGFHPLPGFPDFNDDYCSPVLFPMFRNRILASRRKD"
     gene            complement(238..1179)
                     /locus_tag="PLS35_20770"
     CDS             complement(238..1179)
                     /locus_tag="PLS35_20770"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNI21160.1"
                     /translation="MAIEYPIIAVTPAMVVGDEPLGTKEKFWFEYEGEQWLYKECRAI
                     SGSLLAGEDWSEKIAAEIARMIGILAAKVELADCLGDRGCASRKFTSDTLQLMHGNEI
                     LAEHVLGYEQGRRYGQCQHTLEHIFAAIHKVFPEDGQRREVSAQFAGYCVLDALIGNV
                     DRHHENWGVLWQVGESATMTIGTSIVNPQHQSSRYQLAPSFDHASSLGRELTDQVRER
                     KLANRQVGAYVRRGRGGIYPLNGAQGENPLRLVESAAVCYPDYFRPGLQRLRDTQLSA
                     LQGLVDGVPDDRMSLSAKKFVVELLDVTYTALTRITP"
     gene            complement(1349..>1530)
                     /locus_tag="PLS35_20775"
     CDS             complement(1349..>1530)
                     /locus_tag="PLS35_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21161.1"
                     /translation="RGVDSYLSTLDSQRSLYSAQQNLISVRLARLSNGVDLYKVLGGG
                     AGETEVPAGQVAMNR"
BASE COUNT          367 a          478 c          366 g          319 t
ORIGIN      
        1 agtccttgcg ccttgacgcc aggatgcggt tcctgaacat cggaaacaac accggagaac
       61 agtaatcgtc attaaaatca ggaaatccag gtaacggatg gaaaccgtgc tctttggcgg
      121 caatcacacc ctgtgtataa cgaaaattga aacgctcgcg tccggcatcg gcgtcaaggc
      181 gtccgacagg aaaccaatta cggcttaccg gcgcctgcca ggctagaaac aaggtggtca
      241 aggcgtgatc ctcgtcaaag ccgtgtaggt gacatcgagc aactccacga caaatttctt
      301 ggcagacaaa gacattcgat catccggaac accatcgacc aagccctgca gcgccgaaag
      361 ttgcgtgtcc cgcaagcgct ggaggccagg ccggaagtaa tccggatagc aaacggcagc
      421 cgactccacc agtctgagcg ggttttcacc ctgcgctccg ttcaagggat aaatgcctcc
      481 gcgaccgcga cgcacatacg caccgacctg tctgttcgcc aattttctct cgcgcacctg
      541 atcggtgagc tccctgccca acgatgaagc atgatcaaaa gatggcgcca attgatatcg
      601 gctggattgg tgctgcggat tgacgatgga tgtaccgatc gtcatggtgg cactttctcc
      661 cacttgccac aacacacccc agttttcatg atgccgatcc acattgccga tcaaggcgtc
      721 cagtacgcaa taacctgcaa actgtgctga aacttcccga cgctgcccgt cttccggaaa
      781 aaccttatgg attgcagcaa aaatgtgctc cagcgtatgt tgacactgac cgtagcgtcg
      841 accctgttca tagccaagaa cgtgttcggc cagtatttca ttcccatgca tcaactgcag
      901 agtatcgctc gtaaattttc ttgaggcaca acctctatcc ccaagacaat ccgccaactc
      961 gactttcgcc gccagaatac cgatcatgcg tgcaatttca gccgcgatct tctcggacca
     1021 atcctcaccg gccaatagcg atccagatat cgcccgacat tctttgtaca gccattgctc
     1081 accctcgtac tcaaaccaga atttctcttt ggttcccaag ggctcatctc cgacgaccat
     1141 cgccggagta actgcaatga tcgggtattc aatggccatg actcaatgtc gaactcgatg
     1201 ctgggcagga tcgatgatat ccgactgcga caggccatgc agccagatac ccgataactg
     1261 cgattacatt tcctcaacgc ctcatttctt cgagtaccga caaatacagg aatgcggcca
     1321 ggcatggggc tcacggcaag ctccggtttc accggttcat cgccacctgc cccgccggca
     1381 cctccgtctc ccccgcccca ccgccgagca ccttgtacag atcaaccccg ttcgacagtc
     1441 gcgccagccg cacgctgatc aggttctgct gcgctgagta cagcgagcgc tgcgagtcga
     1501 gcgtgctcag gtagctgtcg acgccgcggc
//
LOCUS       DAOQEW010000424         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_58379_length_1522_cov_3.681663, whole genome shotgun sequence.
ACCESSION   DAOQEW010000424 DAOQEW010000000
VERSION     DAOQEW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58379_length_1522_cov_3.681663"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="PLS35_20780"
     CDS             complement(<1..1285)
                     /locus_tag="PLS35_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21162.1"
                     /translation="MAAIGFSLATLLAPFGACAATNVIAVGKEKQLFLDELFFGYATN
                     VSLRLHPARKTGERILEADQPWENASLNWFSVLQTQGKFRMWYECYDVEGWPTTNDTS
                     FCYAESVDGRHWTKPTLNLFPYHGSRRNNILFREIGPSGAYSRVHGAGVFLDPAAAPE
                     ARYKCVSQGMFAQRQPPYRIAGMTSPDGLHWTRLPSPICDVFADSQYSAFWDVGVNAY
                     VLYGRVSGRGRALGRTVSRDFTRFEPPQLVLENEPARDLYNPAAIKYAGAANAYLMFP
                     SVYDHPSDTLEIHLAVSRDGVHWRFPDPRIPFIARGNPGEFDSGSLYLGQGIVNMRDE
                     LWHYYSGSPLKHEQANLDLMTLPANRRRYSRAVLREDGYVSVQAGEHAGILTTPPLQI
                     QGRRLELNVAVQPGGSLRVGLLDAAGNPIPGLSPDE"
     gene            complement(1337..>1522)
                     /locus_tag="PLS35_20785"
     CDS             complement(1337..>1522)
                     /locus_tag="PLS35_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21163.1"
                     /translation="RLLKEAAAEANVRFVDAGPIAREPANVARSERLFSHDGVAAHPG
                     DRGMKALADAMLQAVLK"
BASE COUNT          303 a          473 c          470 g          276 t
ORIGIN      
        1 attcgtcggg cgacagtccg ggaattggat tgcccgccgc gtccagcagg cccacgcgca
       61 gacttccgcc gggttgcacg gcgacgttca attcaagacg gcgaccctga atctgaagtg
      121 gcggcgtggt caaaatccct gcgtgttcgc cggcttgcac ggagacataa ccatcctcac
      181 gcagcacggc ccggctgtaa cgccggcggt tggctggtag ggtcatcaaa tcaagattgg
      241 cctgctcatg cttgagcggc gagccgctgt agtaatgcca caactcatcg cgcatgttca
      301 cgatcccttg tcccaggtaa aggctgccac tgtcgaactc acccggattt ccccgcgcga
      361 tgaaggggat gcggggatcc gggaaacgcc aatgcacgcc gtcgcggctg acggccaggt
      421 ggatctccaa cgtatcgctt gggtggtcat aaacgctcgg gaacatcagg taggcattgg
      481 cggctccggc gtacttgatg gcggcgggat tgtagaggtc gcgggccggt tcgttttcca
      541 ggaccagttg tggcggttca aagcgggtaa agtcccgact caccgtccgt ccgagcgccc
      601 gaccgcgacc gctcacccgg ccatacaaga cataggcatt cacaccgaca tcccaaaacg
      661 cggagtactg gctgtccgca aagacatcgc agatgggtga cggcaggcgc gtccaatgca
      721 gaccatccgg tgacgtcatc ccggcgatcc gatagggcgg ttggcgttgc gcgaacatgc
      781 cctgcgaaac gcatttgtag cgcgcctccg gggccgccgc cggatcgaga aacacacccg
      841 cgccatggac acgcgaatag gctccgctcg gcccgatttc ccggaacagg atgttgttgc
      901 ggcgactgcc gtgatacggg aacaggttga gcgtgggttt ggtccagtga cgaccgtcca
      961 cggactcggc ataacagaag gaagtgtcat tggtcgtggg ccagccttcc acatcgtaac
     1021 actcgtacca catgcggaac ttcccctgcg tttgcagcac ggaaaaccag ttcagcgacg
     1081 cattctccca aggctggtcc gcctccagga ttcgctctcc ggtcttgcgg gcgggatgca
     1141 gccgcaggga cacattggtc gcatagccaa agaacagctc gtcgaggaaa agctgcttct
     1201 ccttgcccac cgcgatgacg ttggtcgccg cgcacgctcc gaacggagcg agcagagttg
     1261 cgaggctgaa cccgatcgcg gccatgactg cgtggatgcg aactcgatgg agggttggca
     1321 ttggcaaaga tttccttcac ttcaacaccg cttgcaacat ggcatccgcc agggccttca
     1381 ttccccggtc ccccggatgt gcggcgacgc catcatggct gaacaaacgc tccgaccgcg
     1441 cgacgttggc gggctcgcgc gcaatggggc cagcatccac gaagcggacg ttggcttccg
     1501 cggcagcctc tttcagaagc cg
//
LOCUS       DAOQEW010000425         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Nitrospira sp. isolate AMR_MDS_0720
            NODE_59113_length_1512_cov_4.725463, whole genome shotgun sequence.
ACCESSION   DAOQEW010000425 DAOQEW010000000
VERSION     DAOQEW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810352
            Sequence Read Archive: SRR3501873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospira sp. (wastewater metagenome)
  ORGANISM  Nitrospira sp.
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales; Nitrospiraceae;
            Nitrospira.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 03:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,158
            CDSs (total)                      :: 4,125
            Genes (coding)                    :: 4,113
            CDSs (with protein)               :: 4,113
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Nitrospira sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59113_length_1512_cov_4.725463"
                     /isolate="AMR_MDS_0720"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:70125"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-19"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(549..>1512)
                     /locus_tag="PLS35_20790"
     CDS             complement(549..>1512)
                     /locus_tag="PLS35_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNI21164.1"
                     /translation="LALESDPETDSAYWQCVRPEHWLHGEDLPFVLQRLLEAKRPRTA
                     LQCCQYQIDKTDPKLVFSALQQFMAGEEADGPMIDSWHLGEMLEQIEKCGEIEKFALI
                     QLEFGLFPALGYGQEDRARALYESIMSEPQLFVELISLLYKPRHQEREEPLNEGAVTA
                     ATHAWEIFRACKRMPGTGSDGRIDADAFTQFIDSVRELCRKADRQAVGDLTLGEILAH
                     APGDEDGTWPFTPAREILDRPGADDLRRGFSVGVFNKRGTTTRSPCEGGGQERDLACY
                     YRGQAERVQYSHPSVAAMLEEIAKDYEHHGRREDDEAGLRKEGF"
BASE COUNT          328 a          436 c          397 g          351 t
ORIGIN      
        1 tatgcggcga gtgccgccac catgatctac aaggtgaccg acgcttttat cgccaaactg
       61 ccttgatggg tgcaggccgc ggcagggggt gccctgagcg cggcctctgc cccggccgcg
      121 ccctcggtca tcactggaag gccacggcga accagttcgc cagcgtctat ggggagtgct
      181 tcattccggt gaccgcgcga tgaatcaggc cctttctctg cgaaggcggt gggaaggggc
      241 ttcaatggaa tgactcaaca cgatcttttc aaagccaagg atcaggatct gctcgcgtcc
      301 gtggcggcca tcaagcgcgc agcggcgctg gcctcgccaa gcgtgtagcg ccgcgtgccg
      361 gatatcgata tggcagtacc agaggtgtgg cggcgtggag gacggctggt cggccatgca
      421 gccgttcgat tctgtgcaaa actctgaccg ctgagaaatg caaagcggcc cgttaacgcg
      481 actacagaag ttgggacagt tcatccaaag aaccttgcac ggtgatcacg agttctatgg
      541 agcgcatcct agaatccttc ttttcgaaga cccgcctcat catcttctcg cctgccatga
      601 tgctcgtagt ccttggcaat ttcttccagc atggcggcca cactcgggtg actgtattga
      661 acgcgctcag cttggcctcg gtagtaacat gcaagatcgc gctcttggcc acctccttca
      721 caaggagacc gagttgtggt cccgcgcttg ttgaaaactc ccacactgaa tccccgacgc
      781 agatcatccg cccctgggcg atccaaaatc tccctggctg gggtgaatgg ccaggttcca
      841 tcttcgtctc cgggagcatg agccagaatt tcaccgagtg taaggtcgcc tactgcctgc
      901 cgatcagctt ttcgacatag ctctcgaaca gagtcaataa actgagtaaa agcatccgca
      961 tcaatccgcc cgtcgcttcc agtgccaggc atccgtttgc aagctcgaaa aatttcccag
     1021 gcatgggtgg ccgcggttac agctccctcg tttaatggct cttcacgttc ctgatgcctc
     1081 ggcttgtaga gaagcgaaat caattcaacg aacagttgtg gttcggacat tatgctttca
     1141 tacagggctc tggctcgatc ttcctgccca tagccaagag ccggaaacaa tccgaattca
     1201 agttgaatga gcgcaaactt ttctatttcg ccgcatttct caatttgttc aagcatttcc
     1261 cccaaatgcc aggagtcgat cattggtcca tcagcttctt ctccagccat gaactgctgc
     1321 aaagctgaaa agaccagttt tgggtctgtt ttatctatct ggtactggca gcattgaagt
     1381 gctgtccgag gccgctttgc ttctaaaaga cgctgcaaga caaacggcaa atcttcacca
     1441 tgtaaccagt gctcaggccg gacacattgc caataggccg agtctgtttc ggggtcactc
     1501 tccaaggcta gt
//
